LOCUS       DAORLS010000001        46405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_400_length_46405_cov_1.966192, whole genome shotgun sequence.
ACCESSION   DAORLS010000001 DAORLS010000000
VERSION     DAORLS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 46405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46405
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_46405_cov_1.966192"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1791
                     /locus_tag="PK977_00005"
     CDS             <1..>1791
                     /locus_tag="PK977_00005"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF16505.1"
                     /translation="LAAGSNASELLTQVPLVTKDADGKVSVRGKEPKILIDDKPVELN
                     MQQLQDLLESLPGSSIEKIEVMTNPPPQYANEQGGVINIVTKKGKVGKSGRLSVTGGT
                     RGEASVGASFNYRKQGLAIAVNAGYSYNRYAGYGYSIRNNIYKDSSNFFNTTNNYTNK
                     SNRPNFRVNIDYDINKNNLLNLTLNYNQNNFDNYNLTEYQNINRFDELWRLSQRTIQS
                     TGVNNNPNMSLSYTLKGKPGETLRIITSYSLSFNDNDRDFYQQYFNPDYTPNGLDSVQ
                     RQENTTKVNGYTVRVNYDKMLGNKKTFISVGGLYNRSNNHVIIDATYKKKPEGTMEKL
                     PLLSNDFWFHQTVSNLRASVKHIIAENFSFTAGTTAERTDIWFELLKESRDVKNDYLT
                     WLPFANINKTWKEKLSLTFAYRRSIRRPGIGELNPTIDFSDPYNIRFGNANLEASTAH
                     NLDFIIGRTKQKYFLNLGFGYNIVEDVFSQVRTLLDEGITQITWENISGRKEYEVSSW
                     NGITLSKKLKANASASYTYNAYSDFDKTVRKFRNGGSFTSTVGYNFTPSDLWGFTGSF
                     NLNRFASPQGYARWNTSMNLGIQRKFFNKK"
     assembly_gap    1792..1803
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2124..2639)
                     /locus_tag="PK977_00010"
     CDS             complement(2124..2639)
                     /locus_tag="PK977_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16506.1"
                     /translation="MNLEYKYLLDGNFHPESRVWVYQSSRLFSMGEALEIEELLKEFT
                     AKWQSHGTPVKGAAYLFFGQFIILMADETASGVSGCSTDSSVRLIKDIEKRYAVNMFD
                     RTTLAFVIKDKIQLLPLSQVVYAFDNGFIQPGTLYFNNLVQTKEELENNWIIPIQQSW
                     LRKRFDLKAES"
     gene            2685..3077
                     /locus_tag="PK977_00015"
     CDS             2685..3077
                     /locus_tag="PK977_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16507.1"
                     /translation="MTKAHWTYSRQEWKTYMFKKLERKNFLLRTWKKLLLMLAFKTPE
                     VKITPFSIAFGKDRREINQDLRVMDIELMNIDDINIMTVSFDGVKSGKKAEKLLIPVP
                     KGKLRDAFELRQRLLAANQVFSLDQAKC"
     gene            complement(3279..4058)
                     /locus_tag="PK977_00020"
     CDS             complement(3279..4058)
                     /locus_tag="PK977_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRF16508.1"
                     /translation="MQVRTVAELFANGQSPDVLFWVGCAGSFDQRAQKITKAFVTILD
                     KVGINYAILGKEEMCTGDPVRRAGNEFMFQMMAYQNIQILNNYGIKKIITACPHCFNT
                     LKNEYPELGGHYEVIHHTTFLQQLIDEGRIKLKEGGTFKGCKITYHDSCYLGRANDIY
                     EAPRKVLEALDAELVEMKRCKSNGLCCGAGGAQMFKEEEKGTTRINVDRTNEAIGTGV
                     AVIAAACPFCNTMMTDGVKLAEKEETVQVLDVAELVAQSME"
     gene            complement(4125..4640)
                     /locus_tag="PK977_00025"
     CDS             complement(4125..4640)
                     /locus_tag="PK977_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRF16509.1"
                     /translation="MKNILFLFLLFVFSISAHPAVIPASPGIVTDIATKDPVNPYTYL
                     SSMKIREVEKLLGRKMKLKEKLSFKAFQWSIKKGLYPRKPSENSKKGNTAMILGIVAI
                     ASLFIPYLSIASIPCAILAIIFGNQAKKINPNDGQAKAGVILGWVTLGLFVLALIIAL
                     VWLTSGGYWFG"
     gene            4721..>5273
                     /locus_tag="PK977_00030"
     CDS             4721..>5273
                     /locus_tag="PK977_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16510.1"
                     /translation="MKKFFQKITNWELWNFYVLYFPISFVWLWYCIRSGSFWFFSSSN
                     PTITFGGFEGEGKKEMYDQLPPHLVPKTIYVMHDWPIEEVKQRIQDSGFEFPFIVKPD
                     VGMKGILFRKIENEEQLTKYHEKIPVEYIVQDLVLLPVEVSVFYYRYPWEPKGTVSGF
                     IDKELLQVKGNGRSTLKELVETHP"
     assembly_gap    5274..5321
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5322..5751
                     /locus_tag="PK977_00035"
     CDS             <5322..5751
                     /locus_tag="PK977_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16511.1"
                     /translation="IIPEGEIFYLSYAGNHNRGAHFTNLHKEIDESLHKVFDELSHYT
                     DKFYYGRYDIKTTSIEDLKQGKNFFILEFNGCGAEPNHIYDCGMSIWKAYAVILHHWK
                     ALYRISRYNHKNGTPYWSFVRGWKYLRDSAKHFKMLEKYD"
     gene            5820..>6447
                     /locus_tag="PK977_00040"
     CDS             5820..>6447
                     /locus_tag="PK977_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRF16512.1"
                     /translation="MRPLLKIFFTGMLVSFLGSLPLGTLNIAAMQISVSDGVIAAMLF
                     SLGSLLVEIIYVRISLVAMDWIRKQERILKALEWVTLIIVVALAASSFYAALHPKVEK
                     NIVLDSPLPKFVLGLIMSAVNPVQIPFWFGWSTVLFTKKVLLPRKDHYNSYILGIGIG
                     TFIGNCIFIFGGLLIASKINNNQHILNWVIGGIFAITALLQLWKILKKK"
     assembly_gap    6448..6470
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6579..>6819)
                     /locus_tag="PK977_00045"
     CDS             complement(6579..>6819)
                     /locus_tag="PK977_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16513.1"
                     /translation="FAGARVLFSFENFEGQTNRNSLFITPWAEAGYHLVFAKRFFAAP
                     SAGFGYSFKFSKDFHSLDEDVGKRFIPSISAGYRF"
     assembly_gap    6820..6844
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6845..7114)
                     /locus_tag="PK977_00050"
     CDS             complement(<6845..7114)
                     /locus_tag="PK977_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16514.1"
                     /translation="MHLRRILIFIIVSNIPLIIHAQSPKELGAEYIRSIGKVYNTTKV
                     AARGERFDARNSYSAGITYQLASGKSYSVSRGFGVYFGYRYAFGKN"
     assembly_gap    7213..7239
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7240..7872)
                     /locus_tag="PK977_00055"
     CDS             complement(<7240..7872)
                     /locus_tag="PK977_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16515.1"
                     /translation="MKGINGWKKCSKVKCLTAISLFAILTSGCQKQDEAERFYINNTY
                     TIDNSIRVNPIRLFTRTGEINDTLVIHDFVRRNTIGIGFNSPFYMNMSSFPANITFRI
                     SFNSNDSATMTRTSAGLAPDTAIISINKKASFSATQYGLLLRDRDSVSWIFLTDYNYS
                     LVKKSITYYPNRNCGPTIGGMEYCSFNPIIPLKISNDQLFLPITAFIVKSY"
     gene            complement(7838..>8475)
                     /locus_tag="PK977_00060"
     CDS             complement(7838..>8475)
                     /locus_tag="PK977_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRF16516.1"
                     /translation="NYLPYSKHLHIILAFPNAYYARLQPMGKMENMESIQKEVLYAMQ
                     PELAPTGEQAAAPHKFGAKDVMDLSWKNLMDAYSCTECGRCSASCPANQTGKLLSPRK
                     IMMDTRDRLEEVGKNININKEYKDDGKSLLHDYISVEELRACTTCNACVQECPVSISP
                     LDIIVQLRRYLVMEESNSPQEWTAMFGNVENNFAPWKFSPDERDKWVEEMQ"
     assembly_gap    8476..8650
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8651..8960)
                     /locus_tag="PK977_00065"
     CDS             complement(<8651..8960)
                     /locus_tag="PK977_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HRF16517.1"
                     /translation="MHFFVYAGFIIINIEVLEIVLDGITGKHRLFAAPLGGLYTFLIN
                     AFEILALTVLIACVIFLIRRNIIKLKRFISKDLDGWPRSDANYILITEIVLMSLFLLL
                     N"
     assembly_gap    8984..9037
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9250..9660)
                     /locus_tag="PK977_00070"
     CDS             complement(9250..9660)
                     /locus_tag="PK977_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4625 domain-containing protein"
                     /protein_id="HRF16518.1"
                     /translation="MKFRASFLYSITAITMFAAGCSKDDDNTTPADSPAVITTTVPAA
                     NTTYINGSILQVRGNVTDNNGLSTARVEIKNNATNAILYQQNQSTGNVTYYDLNWDWT
                     VTGITSATNATVKITVTDRYNNQVTKDIPVILID"
     gene            complement(9708..>10341)
                     /locus_tag="PK977_00075"
     CDS             complement(9708..>10341)
                     /locus_tag="PK977_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16519.1"
                     /translation="TGMERKKRIWISGLTSFVFQTTIEYLDGRSAEWGWSWADFGANI
                     LGSGTFIAQELAWDEQRIKLKWSFHRKNYSDPLLNQRSNDLFGKSTPERILKDYNGQT
                     YWASMSLKSLLPKSKLPEWLCISIGYGAEGMFGGTENIAKDDDGNIIFNRPDIKRYRQ
                     WFLAPDIDLTKIKTNKKGLRFLFTVLSAFKFPAPSLELSNGKLRLNALHF"
     assembly_gap    10342..10493
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10494..10850
                     /locus_tag="PK977_00080"
     CDS             <10494..10850
                     /locus_tag="PK977_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16520.1"
                     /translation="PPPYPITLVPVISQVRDFIWTEVSGFFIKEVESEIFFASGVAKI
                     ISLFSFIAESFCEKENTGINNVNTSLTNSDRNDEVCDARNDESRTGDDLINNKFTFTK
                     LMWIEKVKFNNLGLQL"
     gene            10859..>11366
                     /locus_tag="PK977_00085"
     CDS             10859..>11366
                     /locus_tag="PK977_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRF16521.1"
                     /translation="MTEIIEQKVAAWLNGNFDQATKEVIRKMQAEHPNELADAFYKNL
                     EFGTGGLRGLMGVGTNRMNKYTVGMATQGYANYLKQCFPNGVKVAIAHDSRNNSRFFA
                     ETTANVFAANGIKVYLFEDMRPTPELSFAIRELGCQGGVVCTASHNPKEYNGYKAYWD
                     DGSQLVPPH"
     assembly_gap    11367..11418
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11419..>12319
                     /locus_tag="PK977_00090"
     CDS             <11419..>12319
                     /locus_tag="PK977_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRF16522.1"
                     /translation="SVDDVKWSGGEGNITIIGKELDEKYMEMVKGLSVYPEVIAKHSD
                     LKIVYTPIHGTGIKLVPPVLEKFGFNSVHIVKEQAVPDGNFPTVVYPNPEESEAMSYG
                     LKQAEELNADILCGTDPDADRLAIGVKDSHGKWVLMNGNQTAVLAFNYLIEARKTKGI
                     AKPNDMVITTIVTTPMIDAIAAANGVGCYRVLTGFKWIAEMIRQKEGKENYIIGGEES
                     FGLMIGDKIRDKDGISAVALLCEMAAYEKEKGRSLYEKMIDLYVQYGFYKEHLVSITK
                     KGMDGQQQIAAMMESYRNNPPKAI"
     assembly_gap    12320..12383
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12384..12610
                     /locus_tag="PK977_00095"
     CDS             <12384..12610
                     /locus_tag="PK977_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRF16523.1"
                     /translation="PQTAEEWAIDLPKSNVLQFILEDGSSISARPSGTEPKIKFYFSV
                     NTKLANAAEFDKVNAVLDEKIKAVIADMKL"
     gene            12677..13333
                     /locus_tag="PK977_00100"
     CDS             12677..13333
                     /locus_tag="PK977_00100"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRF16524.1"
                     /translation="MRPTEDTYRHKGLRKKLVQGIQAKGITDDRVLDAILEVPRHFFL
                     DSAFDEVAYEDRAFPIGEGQTISQPYTVAYQSQLLEIKPFMKVLEIGTGSAYQAVVLA
                     EMGAMVYTIERQKKLFDSNDEFKFLKKYPTIKFFYRDGYEGLPTFAPFDRVIITAAAP
                     YVPPKLIEQMKVGGMMVIPVGTGDIQQMKRITKLEGGALKEEVFDRFSFVPMLGGRES
                     "
     gene            complement(13448..14383)
                     /locus_tag="PK977_00105"
     CDS             complement(13448..14383)
                     /locus_tag="PK977_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF16525.1"
                     /translation="MQYVGLDKQIRKNNFNSILLLIAFPALLLLMFYLLFYFTTGEHH
                     EDPNLLFAQVMPFVLIGVGIWFLIAWAGHSAFIRMATGSKPLERMENKRVYNLTENLC
                     IAQGMQMPKLYIIEDDSLNAFASGINQRTYSISLSRGIINKLDDDELEAVIAHELTHI
                     RNRDVRLLIVSIIFVGIFAFLAEMAFRSLRFAGSGRRSKDSKGSGAIILIAIVVTAIA
                     YFLSILLRFGISRKREYLADAGSAEMTKKPYALANALRKISNDPLIEAVESRDVAQLF
                     IDNPKPSTHKSASWDNMFATHPPIEKRIELLEQFV"
     gene            complement(14411..14971)
                     /locus_tag="PK977_00110"
     CDS             complement(14411..14971)
                     /locus_tag="PK977_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRF16526.1"
                     /translation="MPLLIVIGIIVLIIIWIISLYNGLVRLRNRRENAFADIDVQLRQ
                     RHDLVPQLVETVKGYASHEKELLLKVTEARTAAMSATAIDDKIVAEQQLTAALQGLKV
                     QVEAYPDLKANQNFLQLQEELSDIENKLAASRRFFNGATTEYNNAVESFPGNLVARNF
                     GFKREIMFDLGTDNRKTMEEPPKIQF"
     gene            complement(15083..15499)
                     /locus_tag="PK977_00115"
     CDS             complement(15083..15499)
                     /locus_tag="PK977_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16527.1"
                     /translation="MKRFKNLALVMLSLAAILSFASCEKDDDVPAPAPPSIVGLWKGK
                     YGAVSDYPTLGYTMLFRSNGTVRVFDGTDTTIASKAEGTYSISGTTVTTNYTYLSSPN
                     TYSTSATFNSIYTFMEGTYGSGAATSGAGQFFLVKQ"
     gene            15645..>15988
                     /locus_tag="PK977_00120"
     CDS             15645..>15988
                     /locus_tag="PK977_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF16528.1"
                     /translation="MRIIPFAISAAVTAGLVFVLNKQWGTVPPMGKFLSPQHGFWQNA
                     EPANASFDAELNLEGLKGKAEVYFDDRLVPHVFADNDEDLYFIQGYLHAKFRLFQIDL
                     QTKAAAGRASEIA"
     assembly_gap    15989..16133
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16134..>18034
                     /locus_tag="PK977_00125"
     CDS             <16134..>18034
                     /locus_tag="PK977_00125"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF16529.1"
                     /translation="TNGINAYINSLKDSEIPVEYKLLNIKPEKWTNLRTALLLKMMAK
                     MLSSGTESDLANTNAKSIFLPDELKMLYPQVHDSLMPIVPKGTAFEKPGIIPLKPAIA
                     DSLYLGKKDSVLAQEISKPDINNGSNNWVVAGSKTQSGAPILCNDPHLELSLPSIWYE
                     MQLVSPTSNSYGVSLPGSPFVIIGFNDSIAWGVTNSQRDVKDYYEIKFKDKTKQEYWF
                     NGKWEKTILKEEIIKVKGGKDVLDTVAYTVFGPVMFDESFSNDFSAKRNLAVRWTAHD
                     PSNEGKTFYKLNRAKNYDDYTEAIKTFQCPGQNFVFASKSGDIAIWQQGKFPARWDRQ
                     GLYVMPGEDSSFMWQGFIPQAENPHAKNPERGFLESANQRPADSTYPYFIPGSYITPR
                     GITIENKLAGMTGITVNDMMALHGNYFNTLAEDARSTLLTYVKESELDASSKKFLDIF
                     KSWDLQASPDSKGQTVYQCWWDSLEAEIWKDELTKINPQAPWPEEQTTMELLKKDSAL
                     KFVDNINTAEKENLFDVVTTALKKASADLAKKETEGKLEWAKFKNPTVYHLIKDLKGF
                     ARPGLNVGGNGNIINAITHSHGPSWRMIVQLTTPTEAYGVYPGGQNGNPGSRFYDDYV
                     DTWAEGKYH"
     assembly_gap    18035..18161
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18163..18486
                     /locus_tag="PK977_00130"
     CDS             18163..18486
                     /locus_tag="PK977_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16530.1"
                     /translation="MLLAFVSGLYLPWWGIAITSLLVAVLVHQKAGKAFLSGLLGVFL
                     LWAGLAWWIDMKNNGILSKKIATILPLGGNTILLILVTGLFGGLIAGFAAMSGSFLRA
                     TPKGR"
     gene            18557..>18806
                     /locus_tag="PK977_00135"
     CDS             18557..>18806
                     /locus_tag="PK977_00135"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF16531.1"
                     /translation="MRYTFTTLLALLATVIVYSQKIKGIITDKEGKPLSYATVFVKGT
                     NKGANANNEGKYSLKLPPGKYVLVCQYVGYKKEELPVSI"
     assembly_gap    18807..18824
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18913..21048
                     /locus_tag="PK977_00140"
     CDS             18913..21048
                     /locus_tag="PK977_00140"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF16532.1"
                     /translation="MRNAIKKRTYYQDQPEKFECKVYTKGQLRLRSYPKKFLGQKVDF
                     EDGDTSKQKMMYLSETISKYTVNRPDKEKVEVISSRVSGQSDGYGLSAPQFFSFYSNN
                     VFIGNNLNPRGFISPIAENALSYYRYKLEGTFFEDSNLISRIKVIPKRKYEPLFSGYI
                     NIVEEDWRIHSLQLQLTKQSQMELVDTLRIEQLYRPYDKDTWFISSQVVYPAVKILGF
                     DAIGSFVNIYSELKTEPVFTKKTFTNTILKYADNSNKRTIDYWERNRPIPLMAEEQRD
                     YLKKDSLKLARKDPRYLDSLERKRNKVTVPSLVLIGQSFTKEKKRTSLSFEPLLSQVN
                     FNPAEGWVINTGFTWSKRLDSAGAGRKRISVAPNFRYGFTNKHFNAHLTLNYSFGKKY
                     ASSVSLSGGKRVFQFNNNSPIGERGNTLSCLQSEENRIKSYEAIYVRGSYRKGIGDGF
                     TWTVGFQYQDRLPLDNRTDYTWRDKADRAYTPNYPFEIVNENIKRHQVFVTLVGLTWQ
                     PGAKYIELPDRKINIGSKHPVFSLTYIRNYSNLFGSDADFSKWKFSVRDDINLKLMGV
                     FRYRLGMGGFLDNDKVELPDYNHFNGNISSFATEYLNSFQLLPIYRFSNTSKFYALAH
                     LEHNFKGFLTNKIPGIRKLNLYLVAGANGFYLNKTQTTYVELFAGIDNIFKQIRVDFV
                     QTYGAGIPRQSGFKIGLTRFAGQRGDDWP"
     gene            21109..21795
                     /locus_tag="PK977_00145"
     CDS             21109..21795
                     /locus_tag="PK977_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16533.1"
                     /translation="MKKIILFLSISLNQFISNGQELAQVTFSGGSNFSYFSLITDRTV
                     LIRISDNGTVLEWGTEESSFRNSNYYSPKLQPYPGRVEYYTRNADSLSRGKVKSIGSA
                     VITYYDYYESEERKGKIRSIGSQFFDYYNNFYDKLLRGKIKSIGNSLIDYYPSFDNEA
                     VRGKPKTIGSTSITYYSSFDDRLIRGKVKSIGTQQYQWYTSVDRVGYGGGLKTGSFRQ
                     NIGGVTYILQ"
     gene            complement(21799..22638)
                     /locus_tag="PK977_00150"
     CDS             complement(21799..22638)
                     /locus_tag="PK977_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF16534.1"
                     /translation="MLLKDFIPATDVQEFVQLYRIVHLHFKNNDPVPVKAYPPRPEQC
                     LAFYPYDTETVEYKNNGKRVKNIPVVLYGQFNEVTHRLIGHNFLVFQIIFKPGAIYRL
                     TGIPSSLLTNEYMDAENVFSIELREVNEKLYYATDYSAMLEIANIFIRKLISSQKKPH
                     LLIDNACLLLLNSDQGMTLSQLSKEACLSAKQLERKFKERTGVNPKLYQRIIRFDKAF
                     RLKNSHPEFDWLRIAVDCGYYDYQHLVKEYKDFTGMSPIAFHEVENNAPERKFGLSEG
                     YYS"
     gene            complement(22677..23234)
                     /locus_tag="PK977_00155"
     CDS             complement(22677..23234)
                     /locus_tag="PK977_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF16535.1"
                     /translation="MQRKDFLLTSLLAAPALAFSNNISNREKKLSNTEPFIVDAGKSR
                     FGEVVKFLGVHPNDLKISSKDTDGQLSMFEYTGLGKTGPMLHVHLYQDEIFTVVEGEY
                     RFVVGKDTHHLKAGQTIFLPRGIPHTWIQLSDMGKLIYMLQPAGKMEEFFSYMNSLKE
                     RPTEEEMDKIHAAHGMKVVGPPLTL"
     gene            23393..>23710
                     /locus_tag="PK977_00160"
     CDS             23393..>23710
                     /locus_tag="PK977_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16536.1"
                     /translation="MPNKAGGKAFLSNTLRYYTALILVFVSASFASFSQGSPEYGSGI
                     KINLNQEGTRYIRFINWGQIWFRSQQNNPGSLINGEVKNKTWDIGARRLRVITYAQIS
                     PRYL"
     assembly_gap    23711..23769
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23770..24849
                     /locus_tag="PK977_00165"
     CDS             <23770..24849
                     /locus_tag="PK977_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16537.1"
                     /translation="SSSGTGPSGAGKKPQLFFHDVWNEYAVIPAKNAKTGKSNKYNLY
                     VGGGLHYWLGISRMTSASTLNFLTIDAPIFNWPLIEVSDQFMRQFGFYAKGKLGKLNY
                     SAAINKPFATNNTPAYDTWKGRVATDNNGDAKASMQGYFDYQFLEQEANVLPFRVGTY
                     AGTKKVFNIGAGFYHNSDATKSVDASANIQKHDITLLGVDAFADLPVGNKSKNMAVTA
                     YSVFYNYNFGPNYWRTLGIMNTSTGFDPAMPVADRTINGFGDARMFVGTGNIFYTQAG
                     FLLPKGKTNKMRVQPFAAYTYKKFDFLDEGGSYFDAGSNFFIDGHNAKITAQYSTRPI
                     YYLRNGQRLKDGTKGEFLLQLQVYL"
     gene            complement(24873..26249)
                     /locus_tag="PK977_00170"
     CDS             complement(24873..26249)
                     /locus_tag="PK977_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HRF16538.1"
                     /translation="MSFFSREKTIAPAGYNRWKVPIAALSIHLCIGQIYAFSVFNQPL
                     TKVIGIKESAPEDWELTTLGWIFSIALFFLGASAATFGKWMERVGPRMAMFASAICFS
                     SGFLISSLGVQLHQIWLLYLGSGVIGGIGLGLGYISPVSTLIKWFPDRPGMATGMAIM
                     GFGGGGMIASPLSVYLMKQFRSETSTGVAQTFLVMGIIYFLVMMIGAIIVRVPPENWK
                     PKNFDPAKQKVKKLVTHAHVLADNAIKTPQFYFLFFVLMLNVTAGIGVLGQASVMIQE
                     LFSVESVGPAKAIDASEAAKFVMLLSLFNMLGRFFWSSLSDTIGRKNTYIIFFSLGII
                     LYSAIPSIGSSGSVVLFIAAFAIIMSMYGGGFATVPAYLRDLFGTRQVGAIHGRLLLA
                     WSMAAILGPVLINYMRQYQIEVNKLPKAQVYSMTMYVMAGLLAIGLICNLSIRSVHHK
                     HHHKEDKN"
     gene            26469..>26803
                     /locus_tag="PK977_00175"
     CDS             26469..>26803
                     /locus_tag="PK977_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16539.1"
                     /translation="MSCTNSETTPAPAQFKYEEFKDSVLKNNKDTVPDATNIFDAPSF
                     TPGKDSVILLLERIDTMWKRQAALLARLDTMKRGLKQGPGYSEEEKKIIQENIRMVDS
                     FLLARDSAD"
     assembly_gap    26804..26977
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26978..27233
                     /locus_tag="PK977_00180"
     CDS             <26978..27233
                     /locus_tag="PK977_00180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRF16540.1"
                     /translation="VLTKYTSKKFPGGNYMGLGNMPYAVFISNGYAIHGTTPGNFSKL
                     GNRASHGCIRLHPDNAKVFNALVKTVGLKQTWVSVKDSLP"
     gene            27428..27847
                     /locus_tag="PK977_00185"
     CDS             27428..27847
                     /locus_tag="PK977_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16541.1"
                     /translation="MTETIFGIITAVIIIFISRFKPQYLTQKLFAATILVAIAFIYVG
                     FSLKENQVSIIVLECGFALLLYILAIIGYTKNNSLLAYGIMLHGMWDILHHNSFIVTT
                     NVPGYWPLYCFVIDIIDGIYFLVIFKSQKMTESFDKK"
     gene            27925..28071
                     /locus_tag="PK977_00190"
     CDS             27925..28071
                     /locus_tag="PK977_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16542.1"
                     /translation="MEQKIAILMADLSGYTALTEIHGASSAADLVDKYVEIVEKCLVG
                     DSQL"
     assembly_gap    28083..28113
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28114..28593
                     /locus_tag="PK977_00195"
     CDS             <28114..28593
                     /locus_tag="PK977_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16543.1"
                     /translation="PDLLLATADMIMKNTTRQEYFLQVHGGLHYGQVLIRNNSYFGSA
                     INITARIAAKASPGTFWCSRDFINALADNTTTKFHSKQKYRFKNVSEEMEIAEVLNVY
                     DQTVFIDPVCRMLIQEYSAGIQHPQMKHSYFCSHTCLIFLITTRMLMLQPAGLINLH"
     gene            28606..>29076
                     /locus_tag="PK977_00200"
     CDS             28606..>29076
                     /locus_tag="PK977_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF16544.1"
                     /translation="MAESILLVKNMVCQRCVMAVENMLTKLAIKFQKVIIGEVHLADT
                     ITAEQDTSLNSGLAQIGLELIDNKKGGIIEKIKQLVIEKGRNEVNDVEKKMKLSVYLS
                     GKLYHEYTYLSSLFSSIEGRTIENYFIEQRIEKVKELLVYNEMTLLDISVEMDYS"
     assembly_gap    29077..29227
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29716..30204
                     /locus_tag="PK977_00205"
     CDS             29716..30204
                     /locus_tag="PK977_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRF16545.1"
                     /translation="MKNFLLLAVILITMTTHAQITKVYFQASGLTCSMCSNAINKALK
                     SLDFVDKVTANFKDYSFEVSFKLPDNIDFDRMRRKVEDAGFFVSAFFATINFNNLQIS
                     SGLPVKAGATTLLFVNSNPRLLNGEIRVKVLDKGFVSSKEYKNNAISPSLSGPGTYHV
                     SL"
     gene            30236..>30886
                     /locus_tag="PK977_00210"
     CDS             30236..>30886
                     /locus_tag="PK977_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16546.1"
                     /translation="MNRFLTISLLLLSVSGWSQELFSVTEPASNKAARSITFRLDNTM
                     MDDLSSTKVNYHMIPEIMLGISKKLMLSGNLFFSNRSEKFRFEGGSVYAKYRFLSNDA
                     VQKHFRMALFGRAGYNNSDIHQEEINMNGHNTGVEAGIVATGLLRKLALSSSFSYLNA
                     TDNGNNNKFVYGLDKSNAINYTFSIGKLMLPRQYKDYRQTNLNLMAEFMTQVNTGSG"
     assembly_gap    30887..30925
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31103..32059
                     /locus_tag="PK977_00215"
     CDS             31103..32059
                     /locus_tag="PK977_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRF16547.1"
                     /translation="MKKFAFIIPPTVELLDLAGPLQVFTEAKFYGYELSLEFYSYKEE
                     IISTSGLPFNNIKNYMEADLREGDFVFMPGMHNEYVSSMAFKTEKSFFKWLKDCSEKK
                     VFVCSICNGAFALGHAGLLKDTECTTHWRRIEEMQQEFPTAKVITDILFKKSNNVYTS
                     AGISAGIDLALAILEELKGPLFTHKVARGLVVYHRRSERHTQQSIYLDYRNHINPAIH
                     IVQDHMIEHLSDENSIEDLASLVSMSPRNLSRIFKEKTGTTILEYLTQLRLELAKTML
                     NNPDFTIEYIASKCGFKTARQLQRILKANKSGKRKPADGSGS"
     assembly_gap    32331..32340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32341..33098
                     /locus_tag="PK977_00220"
     CDS             <32341..33098
                     /locus_tag="PK977_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRF16548.1"
                     /translation="ILGSVFSGEVENVGEQVKRFSIGDQVFGHTDMHFGAYAEYIAVP
                     ETGSLAIKPENISHNEAAAIPFGAVTALHFIKKAIIKPGQKVLVVGASGAVGSAAVQL
                     ARSFGADVTGVCSTANIALVKSIGANKVIDYTKEDFSQNGEVYDLIFDTVNAVSVTRT
                     LKSLSREGLLVLSAAGMWKMLQGIWFSSTSKKRVLTGLIRHTSEDIVFLKELIETDKF
                     KPVVDSIYSLEQIAEAHAYVEKGHKKGNVVIAI"
     gene            complement(33291..33752)
                     /locus_tag="PK977_00225"
     CDS             complement(33291..33752)
                     /locus_tag="PK977_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF16549.1"
                     /translation="MKKIIVLLGVVAFITTTLSFRILINEDPWKVPEKYEKLKNPVPA
                     DEASIASGKELYNYFCLSCHGTDGKGSGKRALKLDQSPTDFTTEAFQKQSDGALLFKI
                     YSGHKEMPAFKKRMPGNQDAMDGSFGKTRIPGDIVNYVRTFGKKTAHTLHP"
     gene            complement(33958..>34548)
                     /locus_tag="PK977_00230"
     CDS             complement(33958..>34548)
                     /locus_tag="PK977_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF16550.1"
                     /translation="IRHVPEVYLLNTQYRMRKTIAGFSSQYFYNSLLHTAEHLHDTGT
                     HITFIDTAGTGYHEMHGPDGISLQNEGELNITKQVLEQNNFNTASTAFISPYSGQVAA
                     AKEILPAGLRISTIDSFQGQEQENIIVSLVRSNDEGDIGFLKDYRRMNVAITRAKEQL
                     IVIGDCATIGADRFYSEFLTYVENNGTYQTAWEYEI"
     assembly_gap    34549..34667
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34668..>35596)
                     /locus_tag="PK977_00235"
     CDS             complement(<34668..>35596)
                     /locus_tag="PK977_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRF16551.1"
                     /translation="ASLFRDGAAIECFIAGEEPVKGLLLSLDGKNGEFRLFAPDFPDW
                     IEDDGVGIKLAPDTRTNSIMKKVVTEIENNKPLFRLFEQLHDGKETGINERSEQILEG
                     AVDLSTTKLNLSQQQAVEAILKAHPFTIVHGPPGTGKTTTLAEGISQLVRRGEKVLVA
                     APSNTAVDNISKALLKKGIKILRVGNTSKVDPEIFRHTPEGKLANGNAQKEIKQLKIR
                     AEEFRRMALKYKRSFGKAEREQRNLLFKEVKSIRAEIKKIQAYNEEKLLTEAEVIAGT
                     PIGLYDADLNNLHFHSLFIDEAGQCIEPLAWCI"
     assembly_gap    35597..35625
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35626..35814)
                     /locus_tag="PK977_00240"
     CDS             complement(<35626..35814)
                     /locus_tag="PK977_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16552.1"
                     /translation="MHPHILKLLKCIELEEREEANRYKLDQQHSLKQLRNEGLALHPI
                     IVTRKSFGYADYPEISFKL"
     gene            35926..36918
                     /locus_tag="PK977_00245"
     CDS             35926..36918
                     /locus_tag="PK977_00245"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HRF16553.1"
                     /translation="MLAAIFNTRSFSIISVFGLWLLLFPLLVKSTPANKNRISPVKID
                     IPLTVAADSSSFILPFSRAGNLILVKATADTTTGNFVLDTGAPHLVLNITYFRNYPVQ
                     SNEGERNGITGNAFMAGQTTVADFSWGPAHYYRLNADLANLGNIENSKGVRILGLIGM
                     ELLRNFEMIIDYEKNLIYLHRFRRKVPSSYRNELLADTAAYSVVPITITDNRIMLQSV
                     LAGKKIKLIIDSGAETNLLDSRLPDKVFENVSITGRTMLSGAGAGKIEVLKGDLQQLQ
                     IGNRSLANLPVLITNLERTCFSYGGCVDGILGFDFLMLKKIGFNFVTNKMYIWK"
     gene            complement(37137..>37358)
                     /locus_tag="PK977_00250"
     CDS             complement(37137..>37358)
                     /locus_tag="PK977_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16554.1"
                     /translation="DGLFLNLLYPNKTLLLTEGNCRSKGGSSPIKVIRFAGSFIPSKD
                     SRGLARVKEVELFNPTTQPASLIESVYPD"
     assembly_gap    37359..37395
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37396..>37746
                     /locus_tag="PK977_00255"
     CDS             <37396..>37746
                     /locus_tag="PK977_00255"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16555.1"
                     /translation="LRNNRKADRVMLAGSFNNWDPSALSMTKTDSGWIAGVTLKPGKY
                     WYKFIVDGKWITDPDNGYTENDYAGNTNSVYFFTNTVFSLAGYTQAKKVFVAGSFNGW
                     QGNQLNMNKNANGW"
     assembly_gap    37747..37844
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37845..38394
                     /locus_tag="PK977_00260"
     CDS             <37845..38394
                     /locus_tag="PK977_00260"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HRF16556.1"
                     /translation="YPNEYHETNAVITIGTPHLFMLEGFSEAKTVIVTGSFNGWRKDE
                     LYMKKTDRGWELPYVLGKGNYEYKFIVDGKWIADPANPPYGNNKEANSLLVLSPNYTF
                     RLKGYAGAQSVYLAGNFNSWNPSSMAMRKENDDWVFSVYLSKGKHLYKFIVDGKWIID
                     PDNKLWEQNEHDSGNSVIWFNE"
     gene            38483..38704
                     /locus_tag="PK977_00265"
     CDS             38483..38704
                     /locus_tag="PK977_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16557.1"
                     /translation="MYSKVTKTALQFMMSVLMLTVIVSACNNKKDEEKKDEPAKDTTT
                     APAPTPAPTPSDTTNVPDTADTKPVKTPD"
     gene            complement(38728..>39016)
                     /locus_tag="PK977_00270"
     CDS             complement(38728..>39016)
                     /locus_tag="PK977_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16558.1"
                     /translation="LIRLLGILSLLFLFEYLTLLLHPTVARLTHHTPVYEIIIFVGIA
                     AILIPLHHKLEHWFIHKLLHHRHGKQHPKPAVEPKKIVPAENPVKPEKPKR"
     assembly_gap    39017..39256
                     /estimated_length=240
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39257..>39566)
                     /locus_tag="PK977_00275"
     CDS             complement(<39257..>39566)
                     /locus_tag="PK977_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16559.1"
                     /translation="LGHSYLKMGNNEQSLYNYKKAIGYLTEANDDEILCEAMLGLANL
                     YEKTKKYDSAAWYAGYSLTIARADGFLSKELEAAEFLTEHYRKVNSIDSAFTYVSYVK
                     N"
     assembly_gap    39567..39588
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39589..40212)
                     /locus_tag="PK977_00280"
     CDS             complement(<39589..40212)
                     /locus_tag="PK977_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF16560.1"
                     /translation="MRNQHYIYQHSLLRFIIFVSLFFLYTIANGQRTKVDSLARLLSS
                     AKDDTSRVKYMWQLARDIGTYNPDSALRLSQKALYLARKIDYKEGESRSLGILANTFV
                     KIGNYPRALELNLEKLQLEEKRNNPRNMASVLMNIGVVYVLQEDYRNALSYYSKADSI
                     IQQNKVEDLRYYAALNIGDAYNRLDISDSAFSYFSRSLELAKELDDID"
     gene            40371..>40849
                     /locus_tag="PK977_00285"
     CDS             40371..>40849
                     /locus_tag="PK977_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRF16561.1"
                     /translation="MKWHCLDIKEILTVTGSDTEGLTEDKVLQKQAEHGLNVLEEKKK
                     KPAWLLFLGQFTDFMILILLAAAVIAGIVGDLTDTIIIVVIVLLNAVVGFVQEYKAEK
                     AMDALKKIAALQAKVIRNNHVQTIDAAELVPGDIVLLEAGNVVPADLRLVESHSLLV"
     assembly_gap    40850..40875
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <40876..>42674
                     /locus_tag="PK977_00290"
     CDS             <40876..>42674
                     /locus_tag="PK977_00290"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRF16562.1"
                     /translation="LTGESVPVEKSTQQLDDPELPVGDRINMAFKSTLITNGRGKGIV
                     IATGMQTEIGMIARMLQQEESVTPLQKRMSDFGKKLSYIILLICVLLFAVGLLRGEKP
                     LNMLLISISLAVAAIPEALPALITIALARGAKRLVKKNALVRKLPAVETLGSVTFICS
                     DKTGTLTQNKMEVTDWFSPKENFFLHDNIKMLDGLLALNHDVKKTNDKRWQGDPTEIA
                     MVEYFSANYPDEWMMHIKEHFPRVAELPFDSDRKCMTTVHQQKDRLLVVSKGAAESIA
                     EKLTDKNEAKQLLEKAENWAANGIRVIAFAYKWVDKLPEPFSYDVVETDLLFAGLVGM
                     IDPPREEARQAIAECKTAGIRPVMITGDHPATAAAIAKQIGILTGKELVLTGREMKQM
                     DAEEIESKVEQVRVYARVSPEQKLGIIKSLQRKGHFVSMTGDGVNDAPSLKSANIGVA
                     MGINGTDVSKEAAHMILLDDNFATIVKAVKEGRRIYDNIRKFVKYIMTCNGAEIWTLF
                     LAPLVGLPIPLLPIHILWINLVTDGLPGLALAGEKAEKDIMQRPPRKTDESLFAEGIA
                     YHILWVGLLMAGVTLGMQAWAIQDGNAHWQTMV"
     assembly_gap    42675..42684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42685..42972
                     /locus_tag="PK977_00295"
     CDS             <42685..42972
                     /locus_tag="PK977_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HRF16563.1"
                     /translation="VLSISQLGHVLAIRSDRQFLYKQGLFSNPMLLGAVLLTLLLQLA
                     VIYLPFANQIFKTEPLSLNELLICIGASAIVFHAVEAEKWIKQQIRKKKNL"
     gene            43028..43372
                     /locus_tag="PK977_00300"
     CDS             43028..43372
                     /locus_tag="PK977_00300"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HRF16564.1"
                     /translation="MKLFFSFLLLTLIGTAVNAQSKTGKIDSTKKVMTVDASCGQCNF
                     GLKDKGCSLAVRMDGKAYFVDGTSIDDHGDPHSHDGFCEAIRKADVQGEVVNNRFKVT
                     YFKLLPQPAKKD"
     gene            complement(43442..44731)
                     /locus_tag="PK977_00305"
     CDS             complement(43442..44731)
                     /locus_tag="PK977_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRF16565.1"
                     /translation="MKKLLSIIVSGLVPLFIFAQAGHIMQGIGANNMSMGGAATGQPL
                     DINGALHWNPAGISAFDSKIFSANAGLFFSAPELTSTVPTPGGPMSGVTKDDRGVSVM
                     PALAMVWGKKNSKSTFGISAFGISGFGVTFPESNSNPINMPQSMGGFGRIESDYQLLQ
                     VGLTYAYKLSDKFSIGVAPTFNYSSLELSPNPLASPDPAKGYPISDKATALGVGGQVG
                     LFFNSGMGFKFGISYKTEQSFGEFEFENKYVDGSAAPSVTFKMNYPAIFSAGIGYSQK
                     DFDLALDYRLVNYAKTEGFEAKGWTSTASVKGFGWENISVLSAGVQYKGVKKLPLRIG
                     YTYSANPINEDLAMFSVPATAVIKNAFQLGLGYEFSKSFTLNAVYHHGNSSGNTTGPM
                     LSPMMVNHSNPYGAVPNSSVSYNMTTDMIMFGINYSF"
     gene            44959..>46405
                     /locus_tag="PK977_00310"
     CDS             44959..>46405
                     /locus_tag="PK977_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF16566.1"
                     /translation="MNLPALHRSFVKYFLVLLFISTAIVVSAQSLRVEPMHWWVGMKN
                     PSLQLMVHAADVGETVPFVNYPGVTIKKIHKADSKNYLFIDLIVAKNTKPGRFPIQFK
                     RNGKAVYTYDYELMQRKPNASQLKGFTAADVIYLITPDRFANGDASNDVVSTMRETTI
                     DRKNDYGRHGGDIRGIINSLDYIAGMGFTAIWPTPLLENDMKQSSYHGYAITDYYKVD
                     PRFGTMEEYKELADKCRQKGLKLVFDGVVNHSGSQYWWMKDLPFKNWINYPDSLRITN
                     HRRTVNQDIYAAESDKELMVRGWFVETMPDLNQSNPFLAEFLIQNSIWWAETLGLGGI
                     RQDTYPYSQKKFLEQWTCRIVNEYPQFSIVGEEWSTNPLIAAYWQKGKANNTGYNGCM
                     KSTMDFPLQQALWQSLTEPEEWDKGIVKLYEALANDFVYTNPKDLLVFGDNHDMNRIL
                     TFLNNDVDLLQMAMTYILTIRGIPQVYYGTEA"
BASE COUNT        13380 a         8789 c         9619 g        12650 t
ORIGIN      
        1 ccttgctgcc ggtagtaatg ccagcgaact gctgacccag gtgccgcttg ttactaaaga
       61 tgctgatggc aaagtttcgg taaggggtaa agaacccaag atcctgattg atgacaaacc
      121 tgtagagtta aacatgcagc agttgcagga cctgctggaa tcgctgcccg ggagttctat
      181 tgaaaagatt gaagtaatga ccaacccgcc accgcaatat gccaatgaac agggcggtgt
      241 gatcaatatc gttaccaaaa aaggtaaagt aggtaaaagc ggacgactat ccgtgaccgg
      301 gggtaccagg ggagaagctt cagtgggtgc aagttttaac tatcgtaaac aggggctggc
      361 tattgcagta aatgctggtt atagctataa ccgctatgcc ggttatggtt attccatccg
      421 taataatatt tataaagact catccaattt tttcaacaca acgaataatt acaccaataa
      481 aagtaaccgg cccaatttca gggtgaatat tgattatgat atcaataaga ataacctgct
      541 gaacctgaca ttgaattata atcaaaacaa ttttgacaat tataacctga ccgaatacca
      601 gaatattaac cgctttgatg aattgtggcg tctgagccag cgaacgatcc agagtacagg
      661 tgtgaataac aaccctaaca tgagcctttc ttacaccctg aaaggcaaac cgggagaaac
      721 attaaggatc attaccagct acagtctttc ctttaatgac aacgacaggg atttttacca
      781 gcaatatttt aatcctgatt acacaccaaa cggactggat tctgtgcagc gtcaggaaaa
      841 taccactaag gtgaatggtt atacggttcg ggtaaactat gataaaatgc tgggcaataa
      901 gaaaacattt atttctgtgg gcggattgta taaccgttcc aacaatcatg tgatcattga
      961 tgccacctat aaaaagaagc cggaaggtac catggaaaaa ctgccgttgc tgagtaatga
     1021 tttctggttt caccagacag tgagtaacct gagggcatct gtcaaacata tcattgcgga
     1081 gaattttagt ttcacagcgg gcactactgc agaaaggaca gatatctggt ttgaattgct
     1141 gaaagaaagc cgggatgtaa aaaatgatta cctgacatgg cttccttttg ccaatatcaa
     1201 taaaacctgg aaagaaaaac tgagcctcac ttttgcgtat cgccgcagca tacgccgccc
     1261 gggtatcggc gaactgaatc caactattga tttttcagat ccttataaca tacggtttgg
     1321 aaatgccaac ctggaagctt ctactgctca taacctggat tttattattg gcagaacaaa
     1381 gcagaaatat ttcctgaatc ttggttttgg atacaatatc gttgaagatg tattcagcca
     1441 ggtaagaacc ttgctggatg aagggatcac acaaattacc tgggaaaaca tcagcggcag
     1501 aaaggagtac gaagtaagca gctggaatgg tatcactctt tccaaaaaac taaaagctaa
     1561 tgccagtgcc agctacacct ataatgcgta cagtgatttt gataaaacag taagaaagtt
     1621 caggaacgga ggttctttta cttctacggt tggttataat ttcacacctt ctgatttatg
     1681 gggttttacc ggcagcttta acctgaaccg ttttgcatca ccacaaggat atgcccggtg
     1741 gaataccagc atgaatctcg gtattcagcg gaagttcttc aacaaaaaat tnnnnnnnnn
     1801 nnnctgccaa tgccatcgat ccttttgtga accagcaaag gcgtatctat acctatggac
     1861 ccaacttcaa cctggaaagc tttagccaga cacaaacaaa gaattacagg ctgaccattg
     1921 catacaactt caacaatcaa cccaaaaaac cgcctgttaa aaaatagcca cgaatagttt
     1981 agaaaaatgc ccgtcgtacc tgctggcatt gcatttttga atgccgaacg gaagacggga
     2041 ctcgaattcg ctgcttacag tttgggatct gccagccggc gtactaccag gtgagctact
     2101 tccgcaatta agcagaagtt tattcaactt tctgctttta gatcaaatcg ttttcgtaac
     2161 cagctttgtt gtatcggtat gatccagtta ttttccaatt cttcttttgt ttgcaccagg
     2221 ttgttgaagt aaagtgttcc tggttgtata aatccgttgt caaaagcgta aacaacctgg
     2281 gacaggggca gcaattgtat tttatccttt atcacaaagg caagagtggt tcggtcaaac
     2341 atattcacag catagcgctt ttctatatct tttatcaacc ttacagaact gtcagtgctg
     2401 catccgctaa caccgctggc tgtttcatcc gccattaaaa tgataaactg tccaaagaac
     2461 aaataagcag cgccttttac cggcgtacca tggctctgcc atttagccgt aaactctttc
     2521 agcaactctt caatctccag ggcttcaccc attgaaaaaa gacggctgga ctgatacacc
     2581 caaacccttg attccgggtg aaaattaccg tcgagcaggt atttgtattc gaggttcatg
     2641 ccgctaaatt acgacctatt tataaacccc aaataaagca aagcatgaca aaggcccatt
     2701 ggacctacag caggcaagaa tggaaaactt atatgttcaa aaagctggaa agaaaaaatt
     2761 ttctcttgcg aacatggaaa aaattactgt tgatgcttgc atttaaaaca cctgaagtga
     2821 agattacccc gtttagtatt gcctttggta aagatcgaag ggaaattaac caggatcttc
     2881 gggtcatgga tatcgaacta atgaatattg atgatatcaa tattatgacc gtaagttttg
     2941 atggcgtcaa gtctggcaaa aaagcagaga aattgctcat accagttcct aagggaaaac
     3001 taagagatgc attcgagcta aggcagcgat tgctcgctgc taatcaggtt ttctcactgg
     3061 atcaagccaa atgctaacta ttaaccccag caaaatggac ttgccggtga gttttaaaat
     3121 ctcaaggaaa gtaaaggtat caacagcact aatattccga tcgaaataat cccaggtaat
     3181 tttgaaaatg aaattgtaaa gaagaaatat aacagtaaac tttacaaacc tcttccaggt
     3241 aaaatttctc caaaaccctt ttcttgtttc atacatattt attccattga ctgagccact
     3301 aattcggcca catccagcac ctgtactgtt tcctcttttt cggccagctt tactccgtct
     3361 gtcatcatgg tattacaaaa agggcaggcc gcagcgatta cagctacacc agtgccaata
     3421 gcctcattcg ttctgtcaac atttattcgg gtggttcctt tttcttcttc tttaaacatt
     3481 tgtgctcctc cggctccgca gcagaggcca ttgcttttac agcgtttcat ttctaccagt
     3541 tcggcatcaa gggcttccag cactttacgg ggggcttcat aaatgtcatt ggcacggcca
     3601 agataacagc tgtcgtgata ggtgatcttg caacctttaa aagtgcctcc ttcttttaat
     3661 ttaatccttc cttcatctat tagttgttga agaaaagtag tgtgatgtat gacctcgtaa
     3721 tggcctccaa gttccgggta ttcgtttttg agtgtattga aacaatgcgg gcaggcggtg
     3781 ataatttttt tgatgccata attattaagg atctgaatat tctggtaagc catcatctgg
     3841 aacatgaatt cattaccagc ccttcttacc gggtcaccgg tacacatttc ttcttttcca
     3901 agaatggcat aattaatacc aactttatca aggatggtta caaatgcttt tgtaatcttt
     3961 tgtgcccgtt gatcaaaact accggcacag cctacccaaa ataaaacatc agggctttgt
     4021 ccattggcga ataattcagc tactgtacga acctgcatgg ggaatattta tgggcagcaa
     4081 attactgaat ttgattcttg attaagcaaa ctgttccgaa agaatcagcc aaaccaatac
     4141 cctccgctgg tcagccatac caatgcaata attagcgcca aaacaaaaag acctagcgtt
     4201 acccagccga gaataacacc ggcttttgcc tggccatcat ttggattgat cttttttgcc
     4261 tggttaccga aaataatcgc cagtatggcg catggtatgg aagcgatgct caggtaagga
     4321 ataaaaagag aggcaatagc aacaatacct aatatcatgg cggtatttcc tttttttgaa
     4381 ttttcggatg gctttcgggg ataaagtcct tttttaatac tccattgaaa ggctttgaat
     4441 gataattttt ctttgagttt cattttgcgg cccagtaact tttccacttc cctgatcttc
     4501 attgagctta aataagtgta ggggtttacc gggtctttgg ttgcgatatc agtaactatt
     4561 cccggcgaag caggtataac tgcgggatgt gctgatatgg agaaaacaaa aagcaggaat
     4621 aagaacaaaa tatttttcat ggcagcaggt ttaaaaaaac aatgataaaa accattgttt
     4681 acttgcagga aacctatttt tgtttttttg ctgcatgaag atgaagaaat tttttcagaa
     4741 aataactaac tgggaactct ggaactttta tgtactctat tttcccatca gtttcgtatg
     4801 gctgtggtat tgcatacgca gcggctcttt ctggtttttc agttcatcca atcccaccat
     4861 taccttcggt ggatttgagg gagaaggcaa aaaagaaatg tatgatcagt tgcccccgca
     4921 tctggtgcca aagaccattt atgttatgca cgactggccc atagaagaag tgaaacaaag
     4981 aatacaggat tcaggttttg aatttccatt tattgtaaag cccgatgtag ggatgaaagg
     5041 tattctcttt cggaaaatag agaatgaaga acaattgaca aagtaccatg agaaaatacc
     5101 tgttgaatat attgtacagg atcttgtttt actgccggta gaggtaagtg tattctatta
     5161 ccgatatccg tgggagccaa aaggtacggt aagtggtttt atcgataaag aattattgca
     5221 ggttaaaggg aatggccggt ctaccttgaa agaactggta gaaacccatc caannnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncattattcc tgaaggagaa
     5341 atattttact tgtcttatgc cggcaatcat aaccggggtg ctcattttac caacctgcat
     5401 aaggaaattg atgaaagcct tcataaggtt tttgatgaac taagtcatta tacagacaaa
     5461 ttctattatg gacggtatga tattaaaacc acctctatag aagatctgaa acagggtaaa
     5521 aactttttta tccttgaatt caatggctgc ggtgctgagc ccaatcatat ttatgattgt
     5581 ggtatgagca tctggaaagc atatgctgtc atccttcatc actggaaagc cctttaccgt
     5641 ataagccgct acaatcacaa aaatgggact ccttactggt cctttgtccg gggttggaaa
     5701 tacctcaggg attctgcaaa gcattttaaa atgctggaga agtacgatta ggcgaatgat
     5761 gaagtcggag gtttgacttc cgaccaccaa tttcctgctt cctgccttaa cttcgtagca
     5821 tgcgtccgtt gcttaaaatc ttttttacag gtatgcttgt cagtttcctg ggctctttgc
     5881 cattgggaac actaaatatt gcagcgatgc agatatctgt atcagacggg gtaatagcag
     5941 ccatgttatt ttcattaggg tctttgttgg ttgaaatcat ttatgtccgc atatcactgg
     6001 tagcgatgga ctggatacga aagcaggaga ggattttaaa agcactggag tgggttacac
     6061 taatcatcgt tgtagcactg gcagcatcta gtttctacgc agccctgcat cctaaagtgg
     6121 aaaagaacat tgtactggac agtccgcttc ccaaatttgt gttgggcctt atcatgagtg
     6181 cagttaaccc cgtgcaaata cctttctggt ttgggtggag cactgttctt tttaccaaga
     6241 aggttttatt accgagaaaa gatcattaca attcatatat actcggtatc ggtattggaa
     6301 cctttatcgg gaactgtatt tttatttttg gtggtttatt gattgcctca aagatcaata
     6361 acaaccagca tatcttgaac tgggttatcg gcggcatatt tgcgattaca gctttgttgc
     6421 agctatggaa aatactgaag aaaaaagnnn nnnnnnnnnn nnnnnnnnnn tcaccagatg
     6481 gagcacccgg aagaacttac ccataagata gaggagcaaa tggacaagat cgaaaaaata
     6541 gaagagggga tcgaaaagtt aacccaacct gataaacatt aaaaccggta gcctgccgaa
     6601 atagaaggaa tgaatcgttt tcccacatct tcatccagcg aatgaaaatc cttactgaat
     6661 ttaaaactat agccaaaccc ggctgaaggc gctgcaaaaa accgtttggc aaacactaaa
     6721 tgatagccag cttcagccca tggcgtaatg aaaagactgt tacggtttgt ttgaccttcg
     6781 aaattctcaa aagagaagag tacccttgct ccggcaaagn nnnnnnnnnn nnnnnnnnnn
     6841 nnnngttctt tccaaaagcg tagcggtaac caaaatagac accaaaccct cttgatacag
     6901 aataggattt accggaagcc agctggtaag tgatcccggc gctgtaactg ttcctcgcat
     6961 caaatctttc acctcttgct gctactttgg tggtgttgta aacctttcca atagatcgga
     7021 tatactctgc gcccagttct tttggagatt gggcatgtat tatcagcggt atgttgctaa
     7081 caataataaa tattaaaatt cttctcagat gcattatgct tattcttttt taagtcttgc
     7141 ttcgtttgtt tgtacagcta aggtatcctg attggagagc tgacttaaat ctggttcttt
     7201 aaatgtgttc atnnnnnnnn nnnnnnnnnn nnnnnnnnng tatgatttta ctataaaagc
     7261 tgtaatagga agaaacaact ggtcattgct gatctttaat ggtattattg gattaaatga
     7321 acaatactcc atgccgccaa ttgtgggacc acaatttctg tttgggtaat atgtaatact
     7381 ttttttgact aagctataat tataatctgt caaaaaaatc catgatacac tgtctctgtc
     7441 ccttaataaa agtccatact gtgtagcaga aaaacttgct tttttattaa tagagataat
     7501 cgctgtgtct ggtgccagac ccgcagaagt cctagtcatc gttgccgagt cgttggaatt
     7561 aaaagatatt ctgaaagtaa tattagcagg gaaactactc atgttcatat aaaaaggact
     7621 attaaaacca attccaattg tgtttctgcg aacaaaatca tgaattacta aggtgtcatt
     7681 tatttctcca gttctggtaa ataacctgat tggattaact cttattgaat tgtcaattgt
     7741 gtatgtattg ttgatataaa acctctccgc ttcatcctgt ttttggcaac ctgatgtaag
     7801 tattgcaaat aatgatatgg cggttaaaca tttcacttta ctgcatttct tccacccatt
     7861 tatccctttc atcagggcta aatttccagg gggcaaaatt attctcaaca ttgccaaaca
     7921 tggctgtcca ttcctgcggc gaattacttt cttccattac caggtaacga cggagctgaa
     7981 cgatgatatc cagcggactg atactgacgg ggcactcctg tacacaggca ttgcaggttg
     8041 tgcaggcacg gagttcttct accgagatat aatcatgcag caggctctta ccatcgtctt
     8101 tgtattcttt attgatgtta atattttttc ccacttcttc cagccggtca cgggtatcca
     8161 tcatgatctt gcggggtgaa aggagcttac cggtttggtt ggcggggcag gaagccgagc
     8221 agcggccaca ttctgtacaa ctgtaagcat ccatcaggtt cttccaactc aaatccatta
     8281 catcctttgc gccaaatttg tgcggagcgg ctgcctgctc accggttggg gccagttcag
     8341 gttgcatagc atacaacact tctttctgga tagattccat gttttccatc tttcccatgg
     8401 gttgcaatcg ggcataatat gcattgggaa aggccaggat aatatgcagg tgtttggaat
     8461 agggcaggta gttcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn cattcagcaa aaggaaaaga gacattaaaa caatctctgt aataagaata
     8701 taattagcat cgctccttgg ccatccatcc aggtctttgc tgatgaacct ttttagtttg
     8761 atgatattcc ttcttattaa aaagataaca caggcaataa gtactgttaa tgccagtatt
     8821 tcaaatgcat tgataaggaa tgtataaagt ccgcccaacg gggctgcaaa caaacgatgt
     8881 tttccggtaa tgccatccag cacaatttcc agtacttcaa tattgataat gataaaaccg
     8941 gcatacacaa aaaagtgcat aacagccacc aggggatttc ggannnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg ctgaaatgga ttgtcattta
     9061 ggttttcgtc tcttcccaac cttatgttac ggctgatctc ttttactttt cttgtaaaaa
     9121 gaaaaatgga aacaacgctg attattataa aggcaatttg ctgaagtatt tgcatggcag
     9181 tcaattacag ctgctaattt acgggaagcc cgtctcattc atgccaactg tccaaaaaac
     9241 taaagccaat tagtcgatta gtattaccgg aatatctttt gtaacctggt tattgtagcg
     9301 gtcggtaaca gtgattttta ccgtagcatt cgtggccgat gtgatgccgg ttactgtcca
     9361 gtcccagttc agatcgtaat aagtaacatt accggtagat tggttttgtt gataaagaat
     9421 ggcattggtt gcattattct ttatctcaac ccgggcagtg gataaaccat tattatctgt
     9481 aacatttccc cgtacctgta aaatactgcc attaatataa gtggtattgg cagctggaac
     9541 cgtggtggta attaccgcag ggctgtctgc tggagtagta ttgtcatcat ctttactgca
     9601 acctgcagcg aacatagtaa tagctgtaat tgaataaaga aaggatgccc gaaacttcat
     9661 actatcaatt tgattgaaag gcaatttaca gcaaaacgat gaaacgatca gaaatgcagc
     9721 gcattaagcc ttagctttcc gtttgaaagt tccagtgatg gggcaggaaa tttaaaggcg
     9781 cttaatactg tgaacaggaa acgcaatcct tttttattag ttttgatttt tgtcaggtca
     9841 atatcaggag cgaggaacca ttgacggtag cgtttgatat caggacggtt aaaaattata
     9901 ttgccatcat catcctttgc gatattttct gtaccgccga acataccttc tgcaccatat
     9961 ccgatggaaa tacaaagcca ttcgggcaac ttgcttttcg ggagaagaga tttaaggctc
    10021 atactggccc agtaagtttg accgttataa tcttttaaaa ttctctcagg agtgcttttt
    10081 ccaaacaaat cattacttct ttgattgagt aacggatcgc tgtagttttt ccggtgaaat
    10141 gaccatttta gtttgatacg ttgctcgtcc caggccagtt cctgtgcaat gaatgttccg
    10201 ctccccagga tattggcgcc aaaatcggcc cagctccagc cccattcggc actacggcca
    10261 tctaaatact caatagtcgt ttgaaaaaca aacgaggtaa gaccgctgat ccagatgcgt
    10321 ttttttcttt ccatacccgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccccgc
    10501 catatccgat cacattggtt ccggtgataa gccaggtacg ggactttatc tggactgaag
    10561 tgagtggctt ttttataaaa gaagtggagt ccgaaatttt ctttgccagt ggtgtggcga
    10621 aaattatttc gctattttct tttattgcag aatcattctg tgaaaaggaa aataccggta
    10681 ttaataatgt aaatactagc ctgacgaaca gcgatcggaa cgatgaagtg tgcgacgcaa
    10741 ggaatgatga aagtagaaca ggtgatgacc tcataaacaa caaatttacg tttacaaagc
    10801 tcatgtggat tgaaaaggtg aaattcaata acttagggct tcaattataa acacttttat
    10861 gactgagata atcgaacaaa aagtagcagc ctggctgaat ggaaattttg atcaagcaac
    10921 caaagaagtg atccggaaaa tgcaggcgga gcatcctaat gaactggcag acgcatttta
    10981 caaaaatctt gaatttggaa cgggcggact tcgtggactg atgggtgtag gcaccaaccg
    11041 gatgaataaa tacactgtgg gtatggccac acaaggttat gccaattacc tgaaacaatg
    11101 tttccctaac ggagttaaag tggccattgc acatgacagc cggaacaaca gccgtttttt
    11161 tgcagaaaca accgccaatg tgtttgcggc aaatggtatt aaagtgtatt tgtttgagga
    11221 tatgcggcct actcctgaat tatcctttgc tattcgggaa ttaggttgcc agggtggtgt
    11281 ggtttgtact gcttcgcata atcctaaaga atacaacggc tataaggcct attgggatga
    11341 tggctctcaa ttggtaccac ctcatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnntc cgttgacgat gtgaaatgga gtggcggtga aggcaatatc
    11461 accattattg gaaaggaact ggatgaaaag tatatggaaa tggtgaaagg gctgagtgta
    11521 tacccggaag tgattgccaa acacagtgac ctgaagatcg tttacacacc gatacatggc
    11581 accggtatta agttagtgcc gcctgtattg gaaaaatttg ggtttaatag tgtgcatatt
    11641 gtaaaagagc aggctgttcc tgatggaaat ttccccacgg tggtatatcc taacccggaa
    11701 gaaagtgaag ccatgagtta cgggttaaag caggcagaag aactcaatgc agatattctt
    11761 tgcgggacag acccggatgc ggatcgttta gctattgggg ttaaagattc acatggcaaa
    11821 tgggtgctga tgaacggcaa ccagacagcg gtactggcct ttaattacct gattgaagct
    11881 agaaaaacaa aaggaattgc aaagcccaat gatatggtga ttactaccat cgtaaccacc
    11941 ccgatgattg atgccattgc tgccgcaaac ggtgtgggtt gctaccgggt actgacgggt
    12001 tttaaatgga tcgctgaaat gatcaggcag aaagagggaa aggaaaacta tatcatcggt
    12061 ggtgaagaaa gttttggttt gatgatcggc gataagataa gggacaaaga tggcatcagt
    12121 gcagtagcct tattatgcga aatggcggct tatgaaaaag aaaaagggcg cagtttgtat
    12181 gaaaagatga ttgaccttta tgtgcagtat ggtttttaca aagagcacct ggtctctatt
    12241 accaaaaaag gaatggatgg ccagcagcag atcgcagcta tgatggaaag ctatcgtaat
    12301 aacccgccca aagctatcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnacccgca aacagcagag gagtgggcta ttgatcttcc
    12421 taaatccaat gttttacaat ttatattgga agacggcagc agtatttctg caaggccaag
    12481 tggtactgag ccgaagatca agttctactt tagtgtaaat acaaagcttg ccaatgcggc
    12541 tgagtttgat aaagtgaatg cagtactgga tgaaaagatc aaggcagtaa ttgctgatat
    12601 gaaattgtga tcaccgataa taaatgattt gcaataagca gcatttcgat gctgcttatt
    12661 tatttttgtt cagcttatga gaccaacaga agatacgtat cgccataaag gccttcgcaa
    12721 gaaattagtg cagggtatac aggcaaaagg aattacagat gatagagtgc tggatgctat
    12781 tttggaagtt ccccgtcatt tctttctcga ctcagctttt gatgaagtgg cttatgagga
    12841 ccgggcattc cccattggag aggggcaaac tatttcacag ccctacacag tagcctacca
    12901 aagccagttg ctggaaataa aaccctttat gaaagtgctg gaaattggta cgggcagcgc
    12961 ttaccaggca gtagtgcttg cggagatggg agcaatggtt tatacgattg aaagacagaa
    13021 aaaacttttt gactcgaacg atgaatttaa attcttaaag aaatatccca caatcaaatt
    13081 cttttacaga gatgggtatg aagggcttcc cacttttgct ccgttcgaca gggtcatcat
    13141 caccgcggca gcaccgtatg tgccccccaa attaattgaa cagatgaaag tgggtggtat
    13201 gatggtaata ccggtaggca ccggtgatat tcaacagatg aaaagaatca ccaaactgga
    13261 aggtggcgct ttaaaagaag aagtatttga ccgtttttcg tttgtaccta tgctgggagg
    13321 gagggagtct tgagtaataa gtttggagtc gggagttcag agtctggagt ctggtaattt
    13381 ttatttttca aatgctaatg aggtgagtaa tctggcttct gactattgac taaagactgc
    13441 cgactggtca tacaaactgc tccagtaatt caatcctttt ctcaatcggc ggatgggtgg
    13501 caaacatatt atcccatgag gctgatttgt gggtagaggg tttggggttg tcgatgaaca
    13561 gttgcgccac atcacggctt tctaccgctt ctatcagcgg gtcattggaa atttttctca
    13621 acgcattagc caaagcataa ggtttttttg tcatttcagc gctacctgca tccgccaggt
    13681 attctctttt acggctaata ccaaaacgaa gcagtattga taagaaataa gcaattgccg
    13741 ttactactat tgcaataagg ataatagcgc cgcttccttt gctatcctta ctcctccttc
    13801 ctgagccggc aaagcggagg gagcgaaaag ccatttcggc caggaaggcg aagatgccta
    13861 cgaaaatgat cgaaacgatc agcagccgga catccctgtt tcggatatgc gtcagttcat
    13921 gggcgattac agcttccagt tcgtcatcat ccagtttatt gataatgccc cgtgagaggg
    13981 agatggaata agtacgctga tttattccac tggcaaaagc gttcagggaa tcatcttcga
    14041 tgatatacaa tttgggcatt tgcataccct gtgcgataca gaggttttct gtaaggttgt
    14101 acactctttt gttttccatt ctttccaacg gcttgctgcc ggtggccatg cggataaaag
    14161 cggaatggcc cgcccaggca atcaggaacc agattcctac accgattaat acaaacggca
    14221 ttacctgtgc aaacaacagg ttggggtctt cgtggtgctc gccggtggtg aaataaaaaa
    14281 gtaaatagaa cattaacagc agcagtgccg gaaatgctat taataacagt atggaattaa
    14341 aattattctt ccgaatctgt ttatccagtc ctacgtattg cattttctgc tatccccttc
    14401 aggggtgggg ttaaaattga attttgggtg gttcctccat ggtcttacgg ttatccgtgc
    14461 caaggtcaaa catgatttct cttttgaaac caaagttgcg ggccacgagg ttaccgggaa
    14521 atgattcaac tgcattatta tactccgtag tagcaccgtt aaagaagcgg cggctggctg
    14581 ccagtttatt ttcaatatca ctcaattctt cctgaagctg aagaaaattc tggttggcct
    14641 tcaggtctgg ataggcttct acctgcactt tcagtccctg taaggcagct gtcagttgct
    14701 gttcagctac tatcttatca tcaatagcag tagcagacat cgcagctgtg cgggcctccg
    14761 ttactttcag taacagttct ttttcatgtg aagcataacc ctttactgtc tcaacaagct
    14821 gaggtaccag gtcatggcgt tgccggagtt gaacatctat atctgcaaaa gcgttttccc
    14881 tgcggttgcg taagcggacc aatccattat aaagactgat tatccaaatg atgatcagga
    14941 cgataattcc aataacaatt aatagtggca taatcctgta atttatttct aagttaggca
    15001 aaagatcagt tcactactta atttttaata aacaaaaaaa gggttgccag gcaacccttt
    15061 ttcattcttt tcaaaaaaga tattattgtt tgacaagaaa gaattgtcct gccccgcttg
    15121 tggcagcgcc agatccgtaa gtgccttcca taaaagtata aatggagtta aaagtggcac
    15181 ttgtagaata agtattaggc gatgacagat aggtatagtt ggtagttact gttgttccgc
    15241 tgatactata agtaccctcc gccttactgg caattgttgt atcagttccg tcaaatacac
    15301 gaacggtgcc attactgcgg aaaagcatag tgtagccaag cgtaggatag tcggaaacag
    15361 caccatattt acctttccac agaccaacaa tagaaggagg cgcaggtgca ggaacatcat
    15421 cgtctttttc gcaggatgca aaagataaga tagcggccaa agaaagcatg accagagcca
    15481 ggtttttgaa acgtttcatg gtttagtttt ttagttaatg attaatggta tgcaaaaaaa
    15541 cagatgctta gaatcaaaaa caataccact aactggtatt ttttgctgtt gccgtgaaaa
    15601 tcactttgaa ttattgtgta ttttccgctt ttaatagatt ctcaatgcgt ataattccct
    15661 ttgccatttc tgcagctgta acagctggat tagtttttgt tcttaataaa cagtggggta
    15721 ctgtaccgcc tatgggtaag ttcctgagcc cgcagcacgg attctggcag aatgcagaac
    15781 ctgcgaatgc cagttttgat gcagagctga acctcgaggg cttaaaaggt aaagcagaag
    15841 tatactttga tgaccgcctg gtgccgcatg tctttgcaga taatgatgaa gacctctatt
    15901 ttattcaagg ttacctgcat gcaaaatttc gtttattcca gatcgatctg caaaccaagg
    15961 ctgcggctgg cagggctagt gaaatagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnataccaa
    16141 cgggattaat gcatatataa attctttaaa agattctgaa attccggttg aatacaagtt
    16201 gcttaacatt aaaccggaga aatggacaaa tctgcgtaca gcattattat taaaaatgat
    16261 ggcaaaaatg ttgtcatccg gtacggagag tgaccttgcc aataccaatg ccaaatcaat
    16321 ttttctgccg gatgaattaa aaatgctcta cccgcaggta catgattcat tgatgccgat
    16381 tgttccaaaa ggaaccgcct ttgaaaagcc cggaatcatt ccgttaaagc cagcaatagc
    16441 tgattcatta tacctcggta aaaaggattc ggtgctggca caggaaattt ctaaacccga
    16501 tattaataat ggaagcaaca actgggtggt agctggtagt aaaactcaaa gtggcgctcc
    16561 aatcttgtgc aatgatccgc accttgaatt atcactacct tctatctggt atgagatgca
    16621 attagtatcg cccaccagta attcttatgg tgtttcttta ccgggttcac cctttgtcat
    16681 tatcgggttt aatgacagca ttgcgtgggg agtgacgaat tcacagcggg atgtaaaaga
    16741 ctattacgag attaaattta aagacaagac caaacaagaa tattggttta atggaaaatg
    16801 ggaaaaaacg attttgaaag aggagatcat taaagttaag ggaggaaaag atgtgctgga
    16861 tacagtggcg tatacagtat tcgggccggt gatgtttgat gaaagttttt ccaatgattt
    16921 ttctgccaag cgtaacctgg cagtacgctg gacagctcat gatccaagca atgaaggaaa
    16981 aactttttac aagttgaacc gggcaaagaa ctacgatgac tatacagagg caataaaaac
    17041 attccaatgc cccgggcaga attttgtctt tgcatctaaa tcaggtgata ttgccatatg
    17101 gcagcaggga aaattcccgg cccgctggga caggcaaggc ctttatgtaa tgcccggtga
    17161 ggacagtagt tttatgtggc agggctttat tccacaagca gagaatccgc atgcaaaaaa
    17221 tccagaaaga gggtttttag aaagtgctaa tcagcgtccg gcagattcaa cttatcccta
    17281 ttttattccc ggcagttata ttacaccaag aggtattacc attgagaata aactggccgg
    17341 catgacaggc attacggtga atgatatgat ggcattgcat ggcaactatt tcaatacact
    17401 ggcagaggat gccaggagca ccttgcttac ttatgtgaag gaaagtgaac tggatgcctc
    17461 ttccaaaaaa ttcctggaca tattcaagtc atgggatctg caggcaagcc cggattccaa
    17521 aggtcaaaca gtataccagt gctggtggga tagcctggaa gcagaaatat ggaaagatga
    17581 actgacaaaa attaatccgc aggcaccgtg gccagaagaa caaacaacca tggaactctt
    17641 gaaaaaggat tctgcactga aatttgttga caatatcaat acagcagaga aggaaaactt
    17701 atttgatgta gtaactacgg ctttgaagaa agcttctgcg gatctggcca aaaaagaaac
    17761 agaaggaaaa ctggaatggg ccaaattcaa aaatccaaca gtctaccacc tgatcaagga
    17821 cctgaaagga tttgcaagac ccggattgaa tgtgggcggt aatggtaata ttatcaatgc
    17881 tatcactcac agccatggac ccagttggag aatgattgtg cagttgacta caccgacaga
    17941 agcttacggt gtttatcccg gcggacaaaa tggaaatccc ggcagccgtt tttatgatga
    18001 ctatgtagat acatgggcgg aagggaagta tcatnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttgttact tgcatttgtc
    18181 agcggacttt atcttccctg gtggggtata gccattactt cactcctggt agcagttctt
    18241 gtacatcaaa aagcagggaa ggccttcttg tcaggactcc tgggtgtttt tctattatgg
    18301 gccggacttg cctggtggat agatatgaaa aacaatggca tcctttcaaa gaaaattgcc
    18361 actattcttc ccttaggcgg taatacaatt ttactcattt tggtgaccgg gctttttggc
    18421 gggctgatag ccggctttgc agccatgagc ggtagttttc tgcgggctac tccaaaagga
    18481 cgttaaaatt ttctttccca aagttgtatt gatacctgtt tggtgtttca ttgttatctt
    18541 gaagcaccgc tgtactatga gatatacatt taccacctta ctggcattgt tggcaacagt
    18601 catagtttat tctcaaaaaa ttaaaggtat tattacagat aaagaaggga aaccgctttc
    18661 ttacgccacc gtttttgtaa agggaacaaa caagggggcg aatgccaata acgaaggcaa
    18721 gtattcttta aaacttcctc ccggaaagta tgtgctggtt tgccagtatg ttggatataa
    18781 aaaggaagaa cttcctgttt ccattgnnnn nnnnnnnnnn nnnncaagga aataaatttt
    18841 gtgctgcaac tgcaggaaat ggtactgagt gaagcggtga taaaggcagg ataggaccca
    18901 gcctactcca ttatccggaa tgcaattaaa aaacgaacct attaccagga tcaaccggag
    18961 aaatttgaat gtaaagtata tactaagggt cagttgcgct tacgcagtta tcctaaaaaa
    19021 tttcttggtc aaaaagtaga ttttgaagac ggcgatacca gcaaacaaaa aatgatgtac
    19081 ctctccgaaa ctatttcgaa atatacagtg aaccggccgg ataaagaaaa agtggaagtc
    19141 atctcatcga gggtcagtgg tcagagtgac ggctacggtt tgagtgcacc tcagtttttt
    19201 tctttctaca gcaacaatgt atttatcggg aacaacctga atccaagagg ttttatttcg
    19261 ccgatcgcag aaaatgcact gagttattac cggtacaaac tggaaggcac tttttttgaa
    19321 gacagcaacc tcatcagccg gattaaagtg attcccaaac gtaaatacga gccgcttttc
    19381 agcggttaca tcaatattgt agaagaggat tggagaatac actctttaca attgcagctg
    19441 acaaaacaat cacaaatgga attggtggat acactccgta ttgaacaatt atacaggccc
    19501 tatgataaag acacctggtt tatcagcagc caggtagtat acccggctgt aaaaattctt
    19561 gggttcgatg ctattggcag ctttgttaat atttattcag aactcaaaac agaacctgtg
    19621 tttactaaga agacatttac caatactata ttaaaatatg cggataattc caataagcga
    19681 acaatagact attgggagag aaaccgccca atacctttaa tggcggaaga acaaagggat
    19741 tacctgaaaa aagacagcct gaagctggcc cgcaaagatc ccaggtacct cgattcgctg
    19801 gaaagaaaac ggaacaaggt aacagtacct tctcttgtat taatcgggca atcattcacc
    19861 aaagaaaaaa agagaacttc tttgagtttc gaaccgctgc taagccaggt taactttaat
    19921 ccggccgaag gctgggtaat caatacaggg tttacctggt caaagcggtt ggattcggca
    19981 ggtgccggcc ggaaaagaat atcagtagct ccgaatttcc gatatggctt taccaataaa
    20041 cattttaatg ctcatttgac gctgaattat tcttttggaa agaaatatgc ttcatcggta
    20101 agcttatcag gcggaaaacg ggttttccag tttaataaca acagcccgat aggtgagagg
    20161 ggtaatacac tcagttgcct gcaaagtgaa gaaaacagga tcaagagtta tgaagcaatt
    20221 tatgttaggg gaagctaccg caaaggtatt ggcgatggtt ttacatggac agtaggtttt
    20281 caataccagg acaggttgcc gttggataac cggacagatt acacctggcg ggataaagct
    20341 gaccgggcat acacacccaa ttatcctttc gaaattgtca atgagaatat caaacggcac
    20401 caggtttttg taacattagt cggtttaaca tggcaacccg gtgcaaagta tattgaactg
    20461 ccagaccgga aaattaatat cggttccaaa cacccggttt tttcgttgac ctatatacgt
    20521 aactacagta acctgtttgg cagtgatgca gatttcagca agtggaaatt ttcggtcagg
    20581 gatgatatca atctgaaatt gatgggagtg tttcgctacc gcttaggtat gggcggattt
    20641 ttggataacg acaaagtaga actgccggat tacaaccatt tcaacggaaa tatttccagt
    20701 tttgctactg aatacctcaa tagttttcag ctcctgccga tataccggtt cagcaacaca
    20761 tcaaagtttt atgcactggc acacctggag cataatttta aaggtttttt aaccaataag
    20821 attccgggca ttcggaaatt aaatttgtac ctggtagctg gggccaatgg gttttacctg
    20881 aacaaaactc aaactaccta tgttgaatta tttgccggca ttgataatat attcaagcag
    20941 ataagagtag atttcgttca aacctatgga gccggtatcc ccaggcaaag tggatttaaa
    21001 atagggttaa caaggtttgc cgggcaaaga ggtgatgatt ggccgtgagt aaatggacat
    21061 caggaactca accagatatt ttacgtaatt tggtttcaat aaccccgtat gaaaaaaatc
    21121 attctattcc tttctatatc gctgaatcag tttatatcaa atggccagga actggcacag
    21181 gtaacatttt ccgggggaag caatttttct tacttctcac tgattacgga tcgcactgtg
    21241 ttgataagga tctcagataa tggcactgtg ctggaatggg gcacagaaga atcctccttc
    21301 cggaacagca actattattc gccaaaacta cagccttacc cgggcagggt ggaatattat
    21361 accaggaatg ccgactcctt atcaagaggt aaagtgaaaa gtatcggttc agccgttatt
    21421 acctactatg attattatga atcagaagag agaaagggaa aaataagatc gataggctca
    21481 cagtttttcg attactacaa taatttttat gataagctgc tcagggggaa aattaaatca
    21541 attggtaaca gcctgattga ttactatcct tcctttgata acgaagcagt aagagggaaa
    21601 cctaaaacaa ttggcagtac cagcatcact tattattctt cttttgatga caggctgata
    21661 agaggcaagg tgaagagcat cggtacgcag caataccagt ggtacacttc agtcgacagg
    21721 gtaggatatg gcggaggttt gaagaccggc tccttccggc aaaatattgg gggtgtgact
    21781 tacattctgc aatagcattt aactataata tccctcgctt aatccaaatt ttctttcagg
    21841 tgcattgttt tctacttcat gaaaggctat aggagacatg cctgtaaagt ctttatactc
    21901 ttttacaagg tgctggtaat cgtaatatcc acagtccact gcaatacgca gccagtcaaa
    21961 ttccggatgt gaatttttca gccggaaagc tttgtcaaac cgaataatgc gctgatataa
    22021 tttagggttg acgcctgttc tttctttaaa ctttctttcc agttgtttgg cactaagaca
    22081 ggcctctttg ctgagctggc tgagcgtcat accctgatcg ctgtttagta atagtaaaca
    22141 agcattgtca attaacaaat gaggcttctt ttggctgctg ataagcttac ggatgaagat
    22201 gttggcgatt tccaacatag cggaatagtc agtggcatag tataattttt cattaacctc
    22261 tctgagttcg atcgaaaaaa cattttccgc atccatatac tcattggtaa gtagtgagga
    22321 tggtataccg gtaagtctgt aaatagcgcc aggtttaaat atgatctgaa ataccaaaaa
    22381 attatgacca atcaaacggt gggtcacttc attaaattga ccatataaca caacaggaat
    22441 atttttgacc cgcttgccat tgtttttata ttctacagtt tctgtatcgt aaggataaaa
    22501 ggcaagacat tgctccggtc ttgggggata agccttgact ggaaccggat cgttgttttt
    22561 aaaatgcagg tgaacgatcc tgtacaattg tacaaattcc tgtacatcag ttgctggtat
    22621 gaaatctttt agcagcattg ctatgatgtt gttggaaact caaattaagt taattatcac
    22681 aatgttagcg gtggcccgac tactttcatt ccgtgtgctg catgaatttt gtccatttct
    22741 tcctccgtag gcctttcttt cagggaattc atataagaaa agaattcttc cattttacct
    22801 gctggctgaa gcatgtaaat caatttgccc atgtcactca attgaatcca ggtatgagga
    22861 atgccacggg gcagaaagat agtttgacct gctttaaggt gatgggtgtc ttttcccact
    22921 acaaaccggt attcgccttc aaccaccgta aatatttcat cctggtacag gtgaacatgc
    22981 agcatgggac ctgttttgcc aagtcctgta tactcgaaca tagacaattg gccatctgta
    23041 tcctttgagg atattttcag gtcatttggg tgtaccccca aaaattttac aacttcaccg
    23101 aaacgggatt ttcccgcatc tacaataaac ggttctgtat tggaaagctt tttctctctg
    23161 ttgctgatat tgttggaaaa ggctaatgcc ggcgctgcca gcaaggaagt aagtaaaaag
    23221 tctttacgtt gcatattgtt ggagtttgtg ctgtaaaatt cgacccgcag cgctactgct
    23281 tagtggtaaa aaatggacat tttcatgtac taaagaaaat cataccggta tctgcaaaaa
    23341 atcagccggc agctatgccg ctttttctat ttttatttaa acacacagaa ttatgcctaa
    23401 caaagctggt ggtaaagcat ttttatcgaa cactctccgg tattatacag ccttaattct
    23461 agtatttgtc tctgcttctt tcgcctcgtt ttcacaaggt tcgccagaat atggcagtgg
    23521 tattaagatc aaccttaacc aggaaggtac ccgctatatc cgttttatta actggggaca
    23581 gatctggttt cgcagtcagc aaaataatcc cggttcactt attaatgggg aagtaaaaaa
    23641 caaaacatgg gacatcggtg caagacggct gagagttatt acctatgcac agatttcacc
    23701 ccgctatctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnna gttcttcagg tacaggacca tcaggtgccg gtaagaagcc gcaacttttt
    23821 tttcatgatg tttggaatga gtacgctgta atacctgcaa aaaatgcaaa gaccggtaaa
    23881 agcaataagt ataatcttta cgttggcgga ggtttgcatt actggcttgg tatcagccgt
    23941 atgacgagtg ccagcaccct taatttcctt accatagatg cgcctatttt taactggccg
    24001 cttatcgaag tcagtgacca gtttatgcgg cagtttggtt tttatgcaaa aggtaagttg
    24061 ggaaagctca attattcagc cgcaatcaac aaaccgtttg caacaaacaa cacaccggcc
    24121 tatgatacct ggaaaggaag agtagctact gataacaacg gagacgctaa agcttctatg
    24181 cagggatatt ttgattacca gtttttggag caggaagcca atgtattacc ctttcgggta
    24241 ggtacttatg caggaacaaa aaaggttttc aatattggtg caggttttta tcacaacagt
    24301 gatgctacca aaagtgtgga tgcctcagca aacattcaaa aacacgacat tactttactg
    24361 ggtgtcgatg cttttgccga tctgccagtt gggaacaaat caaaaaatat ggcagttaca
    24421 gcctatagtg ttttttataa ttacaatttc ggccccaatt attggcgaac ccttggtatc
    24481 atgaatacgt ctaccggttt tgatcctgct atgcctgtag ctgaccgcac tattaacggt
    24541 tttggtgatg cccggatgtt cgttggaaca ggaaatattt tctataccca ggccggtttt
    24601 ttattgccca aagggaagac taataaaatg cgggttcagc cttttgctgc ttatacttat
    24661 aagaaatttg actttttgga tgagggcggc agctattttg acgcaggcag caattttttt
    24721 attgacggac acaatgccaa gatcactgca caatacagta cccggcctat ctattattta
    24781 cgcaacggtc aaaggcttaa ggatggaaca aaaggagaat ttttgctgca actgcaggtt
    24841 tatttatagt acaaacataa atcgtgacat tgtcagtttt tatcctcttt atgatgatgt
    24901 ttatggtgta cagacctgat agataaattg catatgagcc caattgccag taatcctgcc
    24961 ataacataca tggtcataga gtaaacctgt gctttaggta gtttattcac ttcaatctgg
    25021 tattgccgca tgtaattaat aagaacggga cccagtatgg cagccatgct ccaggccaat
    25081 aataacctgc cgtgaatagc gccaacctgt ctggtgccga acaaatctct caggtaagcc
    25141 ggaactgtag caaagccgcc accgtacata ctcattataa tggcgaatgc agcgataaac
    25201 aatactacag aacccgaaga gccaatagat ggaatggcag aataaaggat tatacccagt
    25261 gagaagaaga tgatataagt gttttttctg ccaatcgtat cggaaagcga cgaccagaag
    25321 aaacggccaa gcatattgaa caaactaagc agcattacaa actttgctgc ttctgaagca
    25381 tcaatagctt ttgcgggacc taccgattca acagaaaata gttcctgtat cattacggat
    25441 gcctggccca gcacaccaat gccggcggtt acattcagca tcagaacaaa aaacaagaaa
    25501 taaaattgcg gcgttttaat ggcattatca gctaatacat gtgcatgggt caccaatttc
    25561 tttaccttct gctttgcagg atcaaaattt ttaggcttcc agttttccgg tggtaccctt
    25621 acgatgatgg caccaatcat cattactaaa aaataaataa tacccattac aaggaaggtt
    25681 tgtgccacac ctgttgaagt ttcgctcctg aattgtttca tcaggtaaac ggaaagggga
    25741 gaagcgatca taccaccgcc gccaaacccc ataatggcca tacccgttgc catgcccggc
    25801 ctgtcaggaa accattttat aagtgtggat acaggagaaa tataacctag cccaagtccg
    25861 atacctccaa taacacctga cccaaggtat aataaccaaa tctggtgtaa ttgtacaccc
    25921 aatgaagaaa tcaggaatcc gctggaaaaa caaatggctg aggcaaacat ggccatcctg
    25981 gggccgaccc tttccatcca cttgccaaaa gtggcggctg aagctcccag gaaaaagaga
    26041 gcaatactga atatccagcc cagcgtagtc agctcccaat cttcaggagc agattctttg
    26101 atacctataa cttttgtcag cggttgatta aagacactaa atgcataaat ctgccctata
    26161 cacaaatgga tggataaggc ggcaatagga accttccagc ggttatagcc ggcaggtgca
    26221 atcgtttttt cccttgaaaa gaaggacata gttgttgggt tggtggcaca aaatagaagt
    26281 tgtcttattc aggcaggatg attttcggca tgtaaggggc agcttttggt caggtgagtg
    26341 tatagtatca gccaactaat atcattgttc ttacaaatta ctgctgctaa ttttgataac
    26401 ggaggtgtaa aatgttagca ttcagaatag caaccgtact tctacagtgg atcatggtag
    26461 taatttccgt gtcttgtaca aatagtgaaa cgactccggc accggctcaa tttaaatatg
    26521 aagagtttaa agattccgta ttaaaaaaca ataaggatac tgttcctgat gccaccaata
    26581 tattcgacgc cccttccttt acacccggca aagattctgt cattttatta cttgaaagaa
    26641 tcgacactat gtggaaaagg caggcagccc tgctggcaag acttgataca atgaaacggg
    26701 gactgaagca aggacccggc tattccgaag aagaaaaaaa gattattcag gaaaacataa
    26761 ggatggttga ctcatttctg ctggcaagag actcagctga taannnnnnn nnnnnnnnnn
    26821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt acttacaaaa tatacctcca
    27001 agaaatttcc tggaggaaac tacatgggat tgggaaatat gccttacgca gtatttataa
    27061 gtaatggata tgccattcat ggcactactc ccggcaattt ttccaaactg ggaaacaggg
    27121 catcgcatgg atgcatccgc cttcacccgg ataatgcgaa agtgtttaat gcactggtaa
    27181 aaacggtagg tttgaaacaa acctgggtca gtgtaaaaga ctcattgccc tgaagtattg
    27241 aataattgta cttcatcttt tcgaatcatt ttcatccata acccgcattg gttagaaatg
    27301 cattatgtga tcaagtttgt ccgtttctga cagtttcttg tctttagtat ttcttgctcc
    27361 ggagccatct ttgtgctcag aatacgatac tgacccggct tatacttcac ctttaaataa
    27421 aaccagcatg acagaaacaa tattcggaat tattaccgca gttataataa tctttatttc
    27481 gaggtttaaa ccacagtatc tgacccaaaa gttatttgct gcaacaatat tggtggcaat
    27541 agcttttatt tatgtagggt tctcactcaa agaaaaccag gttagtatta ttgtccttga
    27601 atgcggattt gccttactgc tttatatcct tgctattatc ggatacacaa aaaataattc
    27661 attattggca tatgggatta tgctgcatgg aatgtgggat atacttcatc ataattcttt
    27721 tattgtaaca actaatgtgc ccggatactg gccattatat tgttttgtta ttgacattat
    27781 agacgggatt tactttttag tgattttcaa aagccaaaag atgaccgaaa gctttgataa
    27841 aaagtgacga aataacgaag caaaaaaaag ttgaatttat tatccactaa taaacaggaa
    27901 tacctcataa ataatagttc gtatatggag cagaaaattg caatattaat ggccgatctg
    27961 tcaggatata ccgcccttac agaaatacat ggtgcctcat ccgcagcgga cctggtagat
    28021 aaatatgtag aaattgtaga aaaatgtctt gtgggcgata gccagcttta agaacgtaca
    28081 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccgact tactgcttgc aacagctgat
    28141 atgataatga aaaatacaac caggcaagag tattttttgc aggttcatgg tggccttcat
    28201 tatgggcagg tattgattag aaataacagc tattttgggt ctgctattaa cattacagca
    28261 agaatagctg caaaggcaag tcccggtact ttttggtgtt cccgcgattt tattaatgct
    28321 cttgctgaca atacgacaac taagtttcat tcaaaacaaa agtacaggtt caaaaatgtg
    28381 agcgaagaaa tggaaatagc tgaagtatta aatgtatatg atcaaactgt ttttattgat
    28441 cccgtttgca gaatgctgat acaggaatat tcagcaggga tacagcaccc gcaaatgaag
    28501 cactcttatt tttgttccca tacatgtctg atatttttaa taacaacccg gatgcttatg
    28561 ttgcaaccgg cgggcttaat taatctacat tgagaaattt atattatggc agagagcata
    28621 ctgttggtca agaacatggt ttgtcagcgt tgtgtgatgg cggtcgaaaa tatgctgact
    28681 aaactggcaa ttaaatttca aaaagttatt ataggagaag ttcacctggc cgatacaatt
    28741 actgctgaac aagatacatc tttaaactcc ggtctggccc aaattggtct tgaactgatt
    28801 gacaataaaa aaggaggtat cattgaaaaa attaaacagc tggtaataga gaaaggccgg
    28861 aacgaggtga atgacgtaga aaagaaaatg aaactctccg tctatttatc aggaaaatta
    28921 taccatgaat atacttacct cagcagcctt ttctcatcta ttgaaggccg gaccattgaa
    28981 aattatttta ttgaacaaag gatcgaaaaa gtaaaagaat tgctggtgta taatgaaatg
    29041 acattgctgg atatttccgt tgaaatggac tatagtnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29221 nnnnnnnaac aactcctcct tacggttcta cttttggttt aacaaacccg gtcttaacgg
    29281 gatatttaaa aaacaaaaaa acaaaaagta tgaaaccaga aatcaaattg agtaacagta
    29341 ctcaaaaaga cggacaaaaa tgtggtaacc ctgattgtgc ctgcaccaac tgtaattgcg
    29401 gcagcaattg tacatgtaaa ggatgtagat aatcagttat catttcatta ggaggctgtc
    29461 ctgtgaaggg tggccttttt aatttacaaa ttgcctttaa acattagaat aaaaaagtaa
    29521 ttgtgctgca tttttttgat ttctataacc gctgtgttca gcccgataat tcaataattg
    29581 tacaataaac ttgtgtaata tttgtcagtg gctttccggt aataatggaa acagtccgtt
    29641 tttgacatct caacgtcttt ctgaaggata atagtcagtc tacttttaca gtatcaaaac
    29701 aataaacata atacaatgaa aaattttctt ttactcgctg tcattctaat aacaatgaca
    29761 acccatgcac aaattacaaa agtatatttc caggcaagcg gtcttacctg cagcatgtgc
    29821 agtaatgcta tcaacaaagc tctgaaatca ctggattttg ttgataaagt aacggcaaac
    29881 tttaaagatt acagttttga agtatccttc aaattacctg acaatattga ctttgacagg
    29941 atgagaagaa aagtagagga tgccgggttt ttcgtttctg ctttttttgc tacgattaat
    30001 tttaacaatc tgcaaatcag cagcggacta cctgtaaagg cgggtgctac tacattatta
    30061 tttgtcaata gtaatccgag gttactaaac ggtgagataa gagtcaaggt actggacaaa
    30121 ggttttgtta gctcaaagga gtataaaaac aatgctatat ctccttcttt atccgggccc
    30181 ggaacctacc atgtatccct gtaataaaca tttttaaccg cctaattaca ttatcatgaa
    30241 tcgctttcta actattagtc tattactctt atctgtatct ggctggtcac aggaattgtt
    30301 ttccgtaacg gaaccggcaa gtaataaagc agcccgtagt attacattca ggctggataa
    30361 tacgatgatg gatgatttat cctcaacaaa agtcaactat cacatgatcc cggaaattat
    30421 gttgggaatt tccaaaaaac tgatgctgag tggaaacctg ttcttcagca accgtagcga
    30481 aaagttccgc tttgaaggag gcagtgttta tgcgaagtac aggtttttga gtaatgatgc
    30541 ggtacaaaag cactttcgta tggcattatt tggcagggct ggttataata acagtgatat
    30601 tcaccaggaa gaaattaata tgaatggcca taatacggga gtggaagcag gaattgttgc
    30661 taccggcttg ctgcgaaaat tggccctgtc atcttctttt tcttatttaa atgcgacaga
    30721 taacgggaat aacaataagt tcgtatatgg tttggataaa agtaatgcca tcaattatac
    30781 attttctatc ggtaaattga tgttgccacg tcagtataaa gattacaggc aaacaaatct
    30841 gaacctgatg gcggagttca tgacccaggt gaatacggga tccggannnn nnnnnnnnnn
    30901 nnnnnnnnnn nnnnnnnnnn nnnnnggtcc agtttatctt taacagccag ggccggctgg
    30961 atataggtta tagaaaggaa ttaagttcaa cgcttttaag aactgccccg aatggtttct
    31021 tcgtacgcct tgaatacact ttgtttaatg cattttaatg ggttagtgtt gaaagcagct
    31081 aattcgaact taaatattta aaatgaaaaa atttgccttt atcatcccgc caacagttga
    31141 acttttagac ctggcaggac cgctccaggt atttacagaa gccaaatttt atggctatga
    31201 attatcactt gaattttatt cgtacaaaga agagatcata agtacatcgg gactaccctt
    31261 taataatatt aaaaattaca tggaagccga tttgcgggaa ggggactttg tatttatgcc
    31321 cggtatgcat aacgaatatg taagcagtat ggcttttaaa acggagaaga gtttctttaa
    31381 atggctcaaa gattgttcag aaaaaaaagt ttttgtttgc tcgatatgca atggggcatt
    31441 tgcactgggc catgcgggtt tattaaaaga tacagaatgc actacccatt ggagaagaat
    31501 agaggaaatg cagcaggagt tcccaacggc taaagtaatt acagatatac tttttaaaaa
    31561 aagcaacaat gtgtacacca gtgcgggaat cagtgccgga attgatcttg cacttgctat
    31621 actcgaagag ttaaagggcc cgctgtttac gcataaggtg gcccggggcc tggtagtata
    31681 tcaccgaaga agtgaaagac atacacaaca aagtatttat cttgattaca ggaatcatat
    31741 caatccggct atacacatcg ttcaggatca catgatcgaa cacctgtcgg atgaaaactc
    31801 aattgaagat ttggcttcgt tggtttctat gagtcccaga aacctgagca ggatttttaa
    31861 ggaaaagacc ggaacaacca tcctggaata cctgacacag ttgcggctgg agctggcaaa
    31921 aacaatgctt aataatcctg attttactat tgaatatatc gcatcgaaat gcgggtttaa
    31981 aaccgccagg caattgcaaa ggatcttaaa ggccaataaa agcggcaaaa gaaaacctgc
    32041 tgatggttcg ggtagttaaa ctaatttttt atttaaaggc agggagtaag tattcacata
    32101 aagagaataa aaatgaaagc agcatgttat agtaaatatg gcccgccgga ggtactttat
    32161 gttaaagaag cagaaaagcc agttcttcaa aaaaatgaga tattaattaa gattagggct
    32221 accgcagtaa attcaggtga ttgccggtta cgaaaagctg atcctttcgc aataagattt
    32281 gtttttggat tgactaattg ttcagccaat tggcctggtg tccttccctg nnnnnnnnnn
    32341 atatccttgg gagtgttttt tcaggggaag tggaaaatgt aggtgaacaa gttaaacgat
    32401 tcagtatcgg agaccaggtc tttggccata cggatatgca ttttggcgct tatgcagaat
    32461 atattgctgt tcctgaaacg ggttctcttg ccataaaacc tgaaaatatt tcacataacg
    32521 aagcagctgc tattcctttt ggcgctgtca cggcattaca ttttataaaa aaggcaataa
    32581 taaaacccgg gcaaaaagtt cttgtggttg gtgcttccgg ggcggtagga agtgctgctg
    32641 ttcaattggc caggtcattc ggagcagatg ttacaggagt ttgcagcact gccaatattg
    32701 cgttggtaaa atcaatcggt gcaaataagg taattgatta tacaaaggag gatttttccc
    32761 aaaacgggga agtttatgat ttgatttttg ataccgtcaa tgcagtatct gtaacccgca
    32821 cattaaaatc acttagcagg gaaggtctgt tggtgctaag cgctgccgga atgtggaaaa
    32881 tgcttcaggg aatatggttt tcgtcgacta gtaaaaaaag ggtattgaca ggtttaatca
    32941 ggcatacatc tgaagatatt gtttttctca aggaacttat tgaaacagat aaatttaaac
    33001 cggtagttga tagcatatat tccctggaac aaattgcgga agctcatgct tacgttgaaa
    33061 aaggacataa gaaaggtaat gtggtgattg ctatataatc aaaatttaat gcactgtttt
    33121 cttgatgcct tgagggaagt ttaactaaat tgaaatcatt catatggggt gttatctctg
    33181 gtataaataa ggcgctaatt tcagcaaata aagattattt acgctgagca agcaccttat
    33241 ttctaaactg ttttcttgtt acggaatgtc aaaatggtag acagtttcga tcaaggatgc
    33301 aaagtatgtg ctgttttttt accaaatgtc ctgacatagt ttactatatc accgggtatc
    33361 cttgttttac caaagctgcc atccatcgca tcctggttgc ccggcattct tttcttaaat
    33421 gctggcattt ctttatgacc agaataaatc ttgaacagta atgcaccatc agactgcttt
    33481 tgaaaagctt ctgttgtaaa atctgtggga gattgatcaa gtttcaatgc ccttttgcca
    33541 gaaccttttc catcagttcc gtgacaggac aggcagaaat agttatacaa ttctttacca
    33601 gaagcaattg aagcttcatc ggcaggtacc gggttcttta atttttcata cttctcggga
    33661 actttccagg gatcttcgtt tatcaaaatg cggaatgaaa gggtagttgt aataaatgca
    33721 accacaccca gcaatacgat tatttttttc atgttgatta cttgagtggt tactaaaatg
    33781 attccttttc aatttacaac ggatcaacac ccgaatgtta tgacggctgt cagtaaacga
    33841 agaattgttc acaagttttc ttttgtatta tttcgttggt aaactaaaag aaacacgggt
    33901 atctcatttg acatcccggt gacaaacaag caataactat gttcataaag atgatcttta
    33961 gatctcatac tcccatgcag tttggtaagt gccgttattt tctacatagg taaggaactc
    34021 actgtagaac ctgtcggcac ctatcgttgc acagtctcca ataacgatga gttgttcttt
    34081 ggcccttgtg attgcaacat tcatgcggcg ataatctttt aagaaaccaa tatcgccctc
    34141 atcattactt ctgaccaaag aaacaattat gttttcctgc tcctgtccct ggaagctatc
    34201 aatggtactg atgcgaagcc cggcaggtaa aatttcttta gcagctgcca cctgccctga
    34261 atagggggat ataaatgcag tggaagcagt attgaaattg ttttgttcaa gtacttgctt
    34321 cgttatattg agttctccct cattttgcaa actaattccg tccggcccat gcatttcatg
    34381 atatcctgta ccggcggtat caataaaagt gatatgagtg ccggtgtcat gcaaatgttc
    34441 ggcagtatgc aaaagactat tataaaaata ctggctgctg aatccggcaa ttgttttccg
    34501 catcctgtat tgtgtgttca ataaataaac ttctggtaca tgccggatnn nnnnnnnnnn
    34561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa atgcaccagg
    34681 ccagcggctc tatacattgc ccagcctcat caataaacag ggaatgaaag tgcaggttat
    34741 tcaaatccgc atcgtatagg ccaataggtg taccggcaat aacttcggct tctgtcaata
    34801 atttctcttc gttgtaagcc tggatctttt ttatctccgc ccggatactc ttcacctctt
    34861 tgaacaacag gtttctttgc tctctttctg ccttaccaaa actccgcttg tatttcaaag
    34921 ccatacggcg aaattcttca gcccgtattt tcagttgctt tatttctttc tgggcatttc
    34981 catttgccag cttgccttcg ggagtgtgtc ggaaaatttc cggatctact ttactggtat
    35041 tacccacccg taatattttt attccttttt tcagcagtgc ttttgagata ttgtctacag
    35101 ccgtattact gggtgcagca accagtactt tctcacccct tctcaccagt tgagaaatgc
    35161 cttccgcaag ggtggttgtt tttccggttc ccggggggcc atgtacaatt gtgaatggat
    35221 gagctttcag tatagcttca accgcctgct gctggcttag attcagtttt gtagtactca
    35281 ggtcaacagc accttcaagt atttgttctg atctttcatt gatacccgtt tcttttccgt
    35341 catgaagttg ttcaaacagc ctgaataaag gtttattatt ttctatttca gtcaccactt
    35401 ttttcatgat actatttgtc cgggtatctg gtgccagttt aatacctact ccgtcatctt
    35461 caatccagtc aggaaaatcg ggagcaaaca gcctgaattc gccattcttt ccgtccaggc
    35521 taagcaaaag acctttcaca ggttcttctc cggcaataaa gcattcgata gccgctccgt
    35581 ccctgaataa actggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagtt taaaacttat
    35641 ttcagggtaa tcggcatagc cgaagctctt gcgggttaca attatggggt gcagggcaag
    35701 gccttcattc ctgagttgct tcaggctatg ttgctgatcc agtttatagc gattggcttc
    35761 ttctctttct tccagttcta tacatttcag caatttcagg atatgaggat gcatagcggg
    35821 caagataaca aatagtatat tgaaattatt gcagagttga ttgtaacctg ccattcatgt
    35881 aatgcgtccc aatagctatc gtaactatca atatcttgac cccggatgct ggcagctatt
    35941 ttcaatacaa gaagtttctc cataatatcc gtttttggac tgtggttatt attatttccg
    36001 ctgctggtaa aatcaactcc tgccaataag aacaggattt ctcctgtcaa aattgatatt
    36061 ccgttaacag ttgctgcaga ttcttcttct tttatcctgc cctttagcag ggccggaaac
    36121 ctgatcctgg tcaaagcaac agctgataca actaccggca actttgtttt ggatacaggt
    36181 gctccccacc tggtgttgaa tattacttac ttcaggaact atcctgttca aagtaatgaa
    36241 ggggagagaa atggcattac aggaaatgca ttcatggcag gccaaactac agttgccgac
    36301 ttttcatggg gccctgcaca ttattaccgg ctgaatgctg accttgcaaa cctcggtaat
    36361 attgaaaaca gcaaaggggt taggatattg gggcttatcg gaatggaact actcaggaat
    36421 tttgaaatga tcattgatta tgaaaaaaac ctgatctacc tgcatcgttt tcgcagaaaa
    36481 gtaccgtcct cttaccgcaa cgaattgctg gcagatacag ctgcttacag tgttgttccg
    36541 atcacaatca ccgataacag gatcatgttg cagtctgtat tggccggcaa aaaaataaaa
    36601 ctcatcattg acagcggtgc cgaaaccaat ttgctcgaca gccggttgcc ggataaagtc
    36661 tttgaaaatg tatcgataac cgggagaacc atgctgagcg gggcaggggc cggtaaaatt
    36721 gaagtattga aaggtgatct gcagcagttg caaattggca atagatcgct ggccaatctg
    36781 ccggtactga tcaccaacct ggaaagaacc tgtttttctt acggcggttg tgtggatggg
    36841 atacttgggt ttgactttct gatgctcaaa aaaattgggt ttaactttgt aacaaataaa
    36901 atgtatatct ggaaatgatt ggcagtttca tccataaaat aagcagatat ctcctccggc
    36961 cttttgtcgt aatggctatg ctgatcattt ctctggcagg caaagcccag gatgatatta
    37021 agatctgcac tgtcaaagat ggtaatatgt tcatctggct gagtaaaaaa ttaccgcctg
    37081 ccgaactgga tgcttttatt gcgaaatttg atctggggca cctggcatta aaacgcttaa
    37141 tcaggatata cggattctat taaactggca ggctgggtag ttggattaaa taattcaact
    37201 tcttttaccc ttgctaaacc tctggagtct ttcgatggaa taaacgatcc ggcaaaacgg
    37261 atcactttta tgggactgga accgcctttg gatctgcagt ttccctctgt tagtaacagt
    37321 gtcttattcg ggtacaataa gttcaggaac aaaccatcnn nnnnnnnnnn nnnnnnnnnn
    37381 nnnnnnnnnn nnnnnacctg cggaataaca ggaaggcaga tagagtaatg ctggccggca
    37441 gtttcaacaa ctgggaccct tctgccttgt caatgacaaa aactgacagt ggctggattg
    37501 caggtgtaac attaaaaccc ggcaaatact ggtataagtt tatcgtggat ggaaaatgga
    37561 taacggatcc tgataatggt tacacagaaa atgactatgc aggcaatact aattcagtgt
    37621 atttctttac aaacaccgtt ttcagtcttg ccggttatac acaggctaag aaagtttttg
    37681 tagcaggaag ttttaatggc tggcagggga atcagctgaa catgaacaaa aatgcaaatg
    37741 gctgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtatcc aaacgagtac
    37861 catgaaacaa atgccgtgat caccataggg acacctcacc tgtttatgct ggaaggtttt
    37921 tcggaggcca aaacagtgat agtaaccggt tctttcaatg gctggagaaa ggatgagcta
    37981 tacatgaaga aaaccgacag gggatgggaa ctcccttatg tattgggtaa agggaactat
    38041 gaatataaat ttatcgtgga tggcaaatgg attgcagatc cggccaatcc gccatacgga
    38101 aataataagg aagccaactc tttactggta ttgagtccta attatacatt ccggttaaag
    38161 ggttatgccg gggcccaatc agtttacctg gccggtaatt ttaatagctg gaacccgtct
    38221 tccatggcca tgcgaaaaga aaatgacgac tgggtgttct cagtatattt atccaagggg
    38281 aaacacctct acaaattcat tgtggacggt aaatggatca ttgatccgga taataagtta
    38341 tgggaacaaa atgaacatga cagcggcaat tctgtgatct ggtttaatga ataactaaac
    38401 aaggctttct gctatcctcc aaagcttttt ttggaattct actatacttg tcgtacactt
    38461 atgtactaaa atcaaaacta ctatgtactc caaagtcacc aaaactgcac tgcagttcat
    38521 gatgtcggta ttgatgctga cagtaattgt ttcagcctgt aacaacaaaa aagatgaaga
    38581 gaaaaaagat gaaccggcaa aagacactac caccgcaccg gcaccaactc ctgcacctac
    38641 tccgtctgac acaaccaatg tacctgatac tgcagataca aaaccggtaa agactccgga
    38701 ttaaggcttt gttgcagaaa aatgttgtta tcgtttcggc ttttccggct taaccggatt
    38761 ttcagccgga acgatttttt ttggttcaac tgccggttta ggatgttgtt ttccatgccg
    38821 gtgatggagt aatttatgaa tgaaccagtg ttccagcttg tgatgcagcg gtatcaatat
    38881 ggcagcaata cctacaaaaa tgatgatctc gtaaacaggg gtatgatgcg taagtcttgc
    38941 aacagtaggg tgtaataaga gggtaaggta ttcaaacagg aaaagcagtg ataaaatacc
    39001 cagcaatcgt ataagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39241 nnnnnnnnnn nnnnnngatt ttttacataa ctcacatagg tgaaagcact gtcaatactg
    39301 ttgaccttac ggtaatgttc agtcagaaat tctgcagcct ccagttcctt cgataaaaaa
    39361 ccgtctgccc tggcaatggt taaggaatag ccggcatacc aggcggctga gtcatatttt
    39421 tttgttttct cgtaaagatt ggcaagccca agcatggctt cgcatagaat ttcatcgtcg
    39481 ttggcttcgg tcaggtagcc aatcgctttt ttataattat ataaagattg ctcattattt
    39541 cccattttca ggtagctgtg gccgagnnnn nnnnnnnnnn nnnnnnnnat caatatcatc
    39601 cagctctttt gccagttcca gcgaacggct gaaatagctg aaagcggaat ctgaaatatc
    39661 cagccggttg tatgcatcgc caatattcaa tgcagcataa tagcgcaggt cttctacttt
    39721 gttttgctga atgatagaat ctgcttttga ataatagcta agtgcattac gataatcttc
    39781 ctgtaaaaca tataccacac caatgttcat caatacactg gccatattgc ggggattatt
    39841 ccgcttctct tcaagttgaa gtttttcaag gttcagttcc agtgcccttg ggtaattccc
    39901 gatttttaca aaggtgttag ccaaaatacc caaagagcgg gattcgcctt ccttgtagtc
    39961 aatttttcgg gccaggtata aggccttttg agaaagccgg agagcggagt cggggttata
    40021 agtaccgatg tccctggcca gttgccacat gtattttacc ctgctggtat catctttggc
    40081 agatgaaagc aaacgggcaa ggctgtccac ttttgttctt tggccattag caatagtata
    40141 gagaaaaaat aagctgacga aaatgataaa tcgtaaaaga gaatgctggt aaatgtagtg
    40201 ttggttgcgc attggttcaa aaaaagacaa cggcttacaa attagctaat taacacatgc
    40261 catgcaaaaa ctgtttatgc tgccgtacag ttgattttaa tcagccgaaa ggttagttta
    40321 aatgtcgggt tgctgaatta atttgctatt actaactaat gaattgaaaa atgaaatggc
    40381 attgtctcga cattaaagaa attctgacgg ttaccggttc tgacactgaa gggctgacag
    40441 aagataaagt actgcagaag caagccgaac acggcttgaa tgtattggaa gaaaaaaaga
    40501 aaaaaccggc ctggttgctt ttcctgggcc agtttaccga cttcatgatc ctgatcctgc
    40561 tggcagcagc tgtcattgca ggtatagtag gtgatctaac cgacacaatc atcattgtcg
    40621 ttattgtact gctgaatgca gtggtgggtt ttgtccagga gtacaaagca gaaaaggcta
    40681 tggatgcact caagaagatt gccgcactgc aggcaaaagt tatccggaat aaccatgttc
    40741 agactataga tgcagcagaa ctggtaccgg gtgatatagt actcctggaa gctggtaatg
    40801 tagtaccggc cgatctccgt ctggttgaaa gccatagcct gctggtagan nnnnnnnnnn
    40861 nnnnnnnnnn nnnnnattga ctggtgaatc cgttccggta gaaaagagca cccagcaact
    40921 ggatgaccct gaactgcctg ttggcgaccg tatcaacatg gcttttaaaa gcacattgat
    40981 caccaacgga agaggaaagg gaatagtcat tgctaccggc atgcaaaccg aaattggtat
    41041 gattgcccgc atgctgcaac aggaagaatc cgttacgcct ttacaaaaaa gaatgagcga
    41101 ctttggtaaa aagctttcct acatcatcct gctcatctgt gtgctgttgt ttgcagtagg
    41161 attattacgg ggtgagaagc ctctgaatat gttgctgatc tctatttcac tcgcagtggc
    41221 tgctattccc gaagcactgc cggcattaat tactattgca ctggcaagag gtgcaaaaag
    41281 actggtaaag aaaaatgcac tggtcaggaa gctgcctgca gtggaaacgc tgggttctgt
    41341 tacgttcatt tgttccgata aaaccggtac gcttacccag aataaaatgg aggttacaga
    41401 ctggttttcg ccgaaagaga atttcttttt acacgataac atcaaaatgc tggatgggct
    41461 gctggccctg aatcatgatg ttaaaaaaac aaatgacaaa agatggcagg gtgatccgac
    41521 tgagatcgca atggtggaat acttttcagc caattacccg gacgaatgga tgatgcatat
    41581 aaaagaacat tttccaagag tcgcagaact gccttttgac tctgacagga aatgtatgac
    41641 cactgttcac cagcagaaag atcgtttgct cgttgtttcc aaaggtgctg ccgaatctat
    41701 tgcagaaaaa ctcactgaca aaaatgaagc aaagcaattg cttgaaaaag ctgagaactg
    41761 ggcagcaaat ggtatccggg tcatcgcatt tgcgtacaaa tgggtggata aacttccgga
    41821 gccgtttagt tacgatgtgg tagagactga tcttttattt gctgggctgg taggaatgat
    41881 agatccgcca agggaagaag ccaggcaggc tattgcagaa tgtaagaccg ccggcatccg
    41941 ccctgttatg atcacaggcg accacccggc cactgctgca gcgatagcaa aacagatagg
    42001 tatcctcacc ggtaaagaac tggtacttac aggtagggaa atgaagcaaa tggatgcaga
    42061 agagattgaa agcaaagtgg aacaggtgag ggtctatgcc agggtatcac cggagcaaaa
    42121 actcggtatc attaaatcat tgcagcgaaa agggcatttt gtttccatga cgggtgacgg
    42181 agtgaatgat gccccctcac tcaaatcagc caatattggt gtggccatgg gcattaacgg
    42241 caccgatgtg agtaaagaag ctgcacatat gattttgctc gatgacaatt ttgctactat
    42301 tgtaaaagca gtgaaagaag gcagaaggat ctatgataat atccgcaagt ttgtaaaata
    42361 catcatgacc tgcaacgggg cagaaatttg gactcttttt ttagctccgc tggtaggatt
    42421 acccataccg ttgctcccca ttcacatttt atggatcaat ctagttacag acggcctgcc
    42481 cggactggcg ttggcagggg agaaagcaga aaaagatatc atgcagcggc cgcccaggaa
    42541 aaccgatgag agtctttttg cagaagggat cgcttaccat atactttggg taggtttatt
    42601 aatggcgggt gttaccctgg gtatgcaggc atgggccatc caggacggaa atgcacactg
    42661 gcaaacaatg gtatnnnnnn nnnngtgctg tccatttcgc aactggggca tgtactggcg
    42721 atccgcagcg acaggcagtt tttgtataaa cagggtttat tcagtaaccc gatgctgctg
    42781 ggagctgtct tgcttacatt gttgctgcaa ctggccgtaa tttacctgcc ctttgccaac
    42841 cagattttta aaacagaacc gttgtcatta aatgaacttt tgatctgcat cggcgcatct
    42901 gctattgtat ttcatgcggt ggaagcagaa aaatggatca aacaacaaat caggaaaaag
    42961 aaaaatctgt gattaaattg gtagtgacgg ttatctttgt ttcaattaac attaataaac
    43021 caggtatatg aaattgtttt tttctttctt actacttaca ctgatcggta cagcagtaaa
    43081 tgcccagtca aaaaccggaa agatcgactc tactaagaaa gtgatgacag ttgatgcctc
    43141 ctgcgggcag tgcaactttg gattaaaaga caaaggctgc agcctggcag ttcgtatgga
    43201 tggcaaagct tattttgttg acggcacttc tatagatgat catggtgacc ctcattcaca
    43261 tgatggtttt tgcgaagcca tccgcaaggc tgatgtacag ggcgaagtag tgaataatcg
    43321 ctttaaagta acgtacttca aactgctgcc gcaaccggct aaaaaagatt aacaggttca
    43381 attgataata aagaaaaggg ctgatgaatc atcagccctt tattgttaag gagaggttta
    43441 atcagaaact ataattaatt ccaaacatga tcatatctgt ggtcatgtta tatgatacgc
    43501 tgctgttagg aacagcacca taagggttgg aatgattcac catcatcggg cttagcatcg
    43561 ggcctgtagt attgccgctg ctgttgccat ggtggtaaac agcattcagt gtaaagctct
    43621 tgctgaattc ataacccaag cccagctgga atgcattttt gataacagca gtagcaggaa
    43681 cagagaacat ggccagatct tcattgatcg ggttggcgct gtaagtgtaa ccaattctta
    43741 aaggaagttt ttttactcct ttatattgta cacctgcaga taatacagaa atattttccc
    43801 agccaaatcc ttttactgat gcggtggatg tccatccttt agcttcaaaa ccttctgtct
    43861 tagcgtagtt gaccagccgg taatccaatg ccaggtcaaa atctttttgt gaatagccga
    43921 taccagcaga gaagatagca ggataattca ttttgaaggt tacgctgggt gctgcagaac
    43981 catcaacata tttgttttcg aattcaaatt ctccaaatga ttgttctgtt ttgtaagaaa
    44041 taccaaactt gaaccccatt cctgaattga agaacaaacc aacctgtccg cccacaccca
    44101 atgcagtggc tttgtcgctt atagggtaac cttttgcagg atcaggtgaa gctagtggat
    44161 tgggagacag ttcaagtgaa gagtaattaa aggtgggtgc aacaccaata gagaacttgt
    44221 cagaaagttt atacgcatag gtaaggccta cctgcagcaa ctggtaatct gattctattc
    44281 ttccaaagcc acccatactt tgcggcatat tgatagggtt gctgttgctt tccgggaatg
    44341 ttaccccaaa gccgctgatg ccaaaggcag aaatgccaaa tgtgctttta ctgttctttt
    44401 taccccacac catggcaaga gcaggcatta cagaaacacc gcggtcatct ttggtaacac
    44461 cactcatggg gccgccgggt gtaggtacag tagaagttaa ctccggggca gaaaagaaca
    44521 ggcctgcatt agcagaaaat atttttgaat caaaagcgga aataccggca gggttccagt
    44581 gcagcgcacc attgatatct aaaggctgac cggtagctgc gccgcccatt gacatattat
    44641 tggcgccaat gccctgcatg atatgaccgg cttgtgcaaa tatgaatagg ggaactaaac
    44701 ccgaaacaat aattgataat agctttttca tataagtatg ttttgattaa ttcctgctga
    44761 tgcagaaaac atgtaaagct atttttgaaa cagggcgggt atttgtaact cttatcacat
    44821 tgaatgaaaa tcagcagctg aactgataag aaacattatc aatatgacct gcgtcagtta
    44881 aaaaactgta aacagtaagg cttttctatg aaagaataat aagccctttt gaatatctta
    44941 gctgtttaaa tattaaacat gaatttacct gcgctgcatc gttcttttgt gaaatatttt
    45001 ctcgttcttt tatttatttc tactgcaata gttgtctcgg cacaatcctt aagagtagaa
    45061 cctatgcatt ggtgggtggg aatgaaaaat ccttcactgc aactgatggt acatgcagca
    45121 gatgtgggag aaacagttcc ttttgtaaac tatcccggtg taaccattaa gaaaatacat
    45181 aaagccgata gtaaaaatta cctgttcatt gaccttattg ttgcaaagaa tactaagccc
    45241 ggccgttttc ctattcagtt taaaaggaat ggtaaagctg tttatactta tgattacgaa
    45301 ttaatgcaac gtaaacccaa tgccagccag ttgaaaggtt tcactgcagc cgatgtgatc
    45361 tatctcatta ctcccgaccg ttttgccaat ggcgatgcca gtaatgatgt ggtcagtact
    45421 atgcgggaaa ccactataga ccgtaagaat gattatggcc gtcatggtgg tgatatcagg
    45481 ggcattatca acagtctcga ttatatagct ggcatggggt tcaccgctat ctggcctact
    45541 cctttgctgg aaaatgacat gaagcaatct tcctatcatg gttatgccat taccgattat
    45601 tacaaagtgg atcctcgctt tggcaccatg gaagagtaca aagaactggc tgataaatgc
    45661 cggcaaaaag gattgaaact tgtttttgac ggggtggtta accatagcgg atctcaatac
    45721 tggtggatga aagacctgcc ctttaaaaac tggatcaact atcctgattc acttcgcatc
    45781 accaaccatc gcagaacagt aaaccaggat atatatgcag cagaatcaga caaagaactg
    45841 atggtaagag gctggtttgt ggagacaatg cccgatctca accagtcaaa tccatttctt
    45901 gctgaatttt tgatacagaa ttcaatctgg tgggcagaaa cactgggcct cggcggcatc
    45961 cgccaggata cttatccgta ttcacaaaag aaattcctgg aacagtggac ctgccgcatc
    46021 gtaaatgaat acccgcagtt cagtattgta ggcgaggagt ggagtaccaa tccgcttatt
    46081 gctgcttact ggcaaaaagg aaaagctaat aataccggct acaacggttg tatgaaaagt
    46141 actatggatt ttcctttgca gcaggcctta tggcaatcgc tgacagaacc tgaagaatgg
    46201 gacaaaggca ttgtaaaatt atacgaagcc ctcgccaatg attttgttta taccaatccc
    46261 aaagacctgc tggtgttcgg cgacaatcat gatatgaacc gcatcctcac ttttctgaac
    46321 aatgatgtgg atcttttgca aatggccatg acgtatattt taaccatccg cggcattccg
    46381 caggtatatt atggtacgga ggctt
//
LOCUS       DAORLS010000002        40800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_492_length_40800_cov_2.527083, whole genome shotgun sequence.
ACCESSION   DAORLS010000002 DAORLS010000000
VERSION     DAORLS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 40800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40800
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_492_length_40800_cov_2.527083"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PK977_00315"
     CDS             complement(<1..334)
                     /locus_tag="PK977_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRF16567.1"
                     /translation="MKKFAFIPARYAATRFPGKLMQLLGGKSVIRHTYENTVATGLFD
                     EVIVVTDSDIIYTEITAAGGKAVMSQHEHESGSDRIAEAAAEMDVDIIVNVQGDTPFV
                     KKEPLEKLL"
     gene            complement(348..1118)
                     /locus_tag="PK977_00320"
     CDS             complement(348..1118)
                     /locus_tag="PK977_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF16568.1"
                     /translation="MKLLITALAVFISFTLHSQNDPLYVAAKTGLSIREKPDPASKVL
                     DKIPYGTKLNLIDEDGGWTTIITEELTGYWRKVKYNNKTGYIVDSYLLPWPPPKLATV
                     KDMKQYLSQVSVPFGQKLLTRSGATPQIPESGWQLSKQLYKNGAEHHDFLAYEYASDT
                     YFLPGFSLQQGFLLLRLIPEFKDVFGEKDVFPTASKTITKDGREYDIKLFKTVDDPEY
                     GGPFTIEKIRISYDEGASYVFEMYMQENQLVVLLSGGL"
     gene            complement(1138..1956)
                     /locus_tag="PK977_00325"
     CDS             complement(1138..1956)
                     /locus_tag="PK977_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF16569.1"
                     /translation="MWEQIIAHFQTLEQRPLERMAILVGGLLFFWILEGAIPLFRPHY
                     RKNKIRHAGVNFVLTVLHLILHTGLAFFIVKLSDWCLHNQFGLVYWFDAGVLATVLIG
                     VFALDFSSWLVHLVMHKNKFLWRFHVIHHSDTNVDVTTGLRHHPGDSLLRGIFFMLLI
                     FVSGAPMYGVMIYQTLVVLATAFTHANISLPEKVDKALSYFLISPNMHKVHHHWKQPY
                     TDTNYGAVFSIWDRLLGTFSRLNIKEVRYGIDHDFPEERDEDVLLLLKKPFTKS"
     gene            complement(2005..2439)
                     /locus_tag="PK977_00330"
     CDS             complement(2005..2439)
                     /locus_tag="PK977_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRF16570.1"
                     /translation="MEFVLLSSYTNYVEAHIARGVLEEEGINCWLKDENTVTIDPILT
                     NAVGGIKLMVAKSQSERAREILDRLRRESKAAHACPKCGSHNIELVSTPRKASNWLSV
                     LIGIFATSYAPAIESVYHCFDCKHEWPPVDEKKEDNESAANN"
     gene            complement(2449..3225)
                     /locus_tag="PK977_00335"
     CDS             complement(2449..3225)
                     /locus_tag="PK977_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HRF16571.1"
                     /translation="MRKISCLFIAGFLFVACNNDKKSDAPADKQEDKTDTTTQAGTTE
                     LKQTPLQVAVKPYEKLLGSFVGPFGTNKITMLITLIAGDSVVGRTIVGGNDRPFRGTF
                     KKEGDFYYFNAKEPGDHKDDGEFNWVMDDTSPDHINGGWKPYAKGRAEKEYQLQRKKF
                     EYRTDVGTWPESSQRLLKPEDVENLMKEELEFMRNEIFARHGYCFSKKHLRQQFEMEE
                     WYVPNTVDIRGFLTDIEKKNIEMIKRYEKYAEEYGDEYGR"
     gene            3342..3956
                     /locus_tag="PK977_00340"
     CDS             3342..3956
                     /locus_tag="PK977_00340"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRF16572.1"
                     /translation="MAGITIDYIPYNTDVGFLRIKQQYIEIDHWRIAFFIHVYASIWV
                     LLAGFTQFSGWVQRNNPKLHRAMGYIYVADVLLVTGPAGLLMGFYANGGLLSRIAFVL
                     LAFLWIYFTAMALIKAKQKDFKSHRHYMIRSYALTLSALTLRAWKYVITNAYHIDPNW
                     VFPPMDVYRVVAWLGWVVNLGVAEYIVYRSKLKSFYKRRSLEQG"
     gene            complement(4044..5219)
                     /gene="dnaN"
                     /locus_tag="PK977_00345"
     CDS             complement(4044..5219)
                     /gene="dnaN"
                     /locus_tag="PK977_00345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRF16573.1"
                     /translation="MSKVLAVNQKSVVSPQTKNTMKFIASSSSLLKQLQHIAGVINAN
                     TVLPILEDFLFEVEKNKLTVVATDLETVMRVQLDIEAKDSGKVCIPAKILMDSLKNLP
                     DQPLTFNIDKNFGIEITSDNGKYKVMGENPDNFPKEPGADDTTSFTMTASALVTAINK
                     TIFATSNDDLRPAMTGVFFELDKKGLQCVATDAHRLVRYKRTDVKCPKTDSFIAPRKP
                     LTLLKTAIPSNEDEITVSYNSNHLFVKHGTTQMSCRLIDARFPDYKVVIPADNPYKLT
                     VNKSDFQSALRRVSVFSNKSTNQVALSISGSELQLAAQDVDFSFEGNERMKCQYNGED
                     LMIAFNARFLIEMLNATESDEVNIELSTPTKAGIIKPTDQDENEELLMLVMPLMLNQ"
     gene            5315..6637
                     /locus_tag="PK977_00350"
     CDS             5315..6637
                     /locus_tag="PK977_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16574.1"
                     /translation="MFDKLFGWGKKKAGQEAEPEISFGRYSDNNKPLAKVNRWTDADN
                     LFKEKKYPESLDAFFEYLRDDTIQNVVYERNGAEGRFLFYQGSKIVRGSFDGELLKAE
                     VTLARMPQPSVPVMRRLLEMNFNLYYSRFSLDNDRLCMRFDSDAETASPSKLYYGLKE
                     LATKADKQDDLLVQDFTILETSDSEHITVLSENEKLVKYEFLQKWIKQTLDIISPLDA
                     DKFSGGIAYLLLALICRIDYLITPEGKLLHELEKIAGIYFKKDERPVVEKNRDMIEEF
                     KKIQAIPKEEVFKNLFRSKHTFAIVAPQQYKTIADNIHASNQNMMWYRDNNYPFIATQ
                     ICEYGISYCQYSYSLPSVITELFHLFMMVNYPDYFSALGYKTAYYDPASGKFENESII
                     EKVLSIQQKWKEKYPLMDFKTQNLRFDSLVNFDLTFTNEMEFLNMEPK"
     gene            6646..7746
                     /locus_tag="PK977_00355"
     CDS             6646..7746
                     /locus_tag="PK977_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF16575.1"
                     /translation="MDPQLIIEKYQNAIIQIATAGGTGTGFYVKEYDLIVTNDHVVAD
                     NAEVTIAGKTFDKALSRVWYTDRKHDLAFLEAPKNIELPEVRLGLYEQMKDGDVVVAI
                     GHPYGLNYTATQGVISKVDRIREGLKYIQIDAAINPGNSGGPLVNMNGEVIGVNSFII
                     RGGDNLGFALPVNYLREALQLYTPNRGQASTRCFSCGYLVTTQNIDSKKYCPSCGTEV
                     KLPEIPEKEVEAIGAAKTIEEILKELGKDVKLAREGVNNWSVKEGSAKIKITYNPDNY
                     FIAGDAYLCQMPSDATKIKPLYQFLLQENYRMNGLVLSCVKQNIVLSSVMYDLDLTKD
                     NGVEAFSRLFRQADHYDDFLKKEYGCTERLEE"
     gene            7785..8234
                     /locus_tag="PK977_00360"
     CDS             7785..8234
                     /locus_tag="PK977_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRF16576.1"
                     /translation="MTKEQALQKLKHYCAYQERCHSEVKEKLYQLGVWKKDHDEIIAS
                     LIEENYLNEERFAIAYAGGKFRVKQWGRVKIKYELKQKQVSDYSIKKAMKQIDEEEYL
                     SVLNKLAKEKYAALKSEQYLVRKKKTMDYLLAKGFESDLVRKAVENE"
     gene            complement(8247..8990)
                     /locus_tag="PK977_00365"
     CDS             complement(8247..8990)
                     /locus_tag="PK977_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HRF16577.1"
                     /translation="MKPGEFIKFRIFRPLLLKIGAWMGKYSPVGDLPVFDNKDFEWTT
                     LLEENWLTIRKELEGILANHQQFPSLQDIQKEQIVLNQDNNWKTYFLFGFGLKAVKNC
                     EACPVTASLLEKIPDMKTAFFSILYPGKHIPAHKGVFKGLIRSHLGLIIPGKKGDCLM
                     RIDNQKILWEEGKAVVFDDTYEHEVWNNSNQVRVVLLIDIIRPFKTPLAAINKGIINI
                     ISKSFFVKNAQKNYQEWEDKYYAINDGGK"
     gene            complement(8987..>9241)
                     /locus_tag="PK977_00370"
     CDS             complement(8987..>9241)
                     /locus_tag="PK977_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF16578.1"
                     /translation="AVNTRIENGHIVVSVKDEGIGMSPEQIQRLMEDEVVITAANVDN
                     KKGHGLGYLIIKDLLKTMGASLDITSTKGAGTTVSIKMSK"
     assembly_gap    9242..9258
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9259..>9916)
                     /locus_tag="PK977_00375"
     CDS             complement(<9259..>9916)
                     /locus_tag="PK977_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF16579.1"
                     /translation="KMNGFGRDNYSYKEISFVISTPWYQRWWFYAACAGLLAGLVVVY
                     INFRTKQFKIRQRRLEKQVAEKTKELQEQNEILEKNNSIKTRLISIISHDIVTPLKFL
                     TAAGKNLLEKRKLMSEDLQQETVREMTNTSQELQLLSTNILNWIKYQNENRRMVKEVF
                     NLNEMVAQVLGILQTLARQKSLVIENNVDPSIEINQFYEPLKILIYNLLTNAIQFTEK
                     G"
     assembly_gap    9917..9926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9927..>10523)
                     /locus_tag="PK977_00380"
     CDS             complement(<9927..>10523)
                     /locus_tag="PK977_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16580.1"
                     /translation="NGLYISKNGKVKPLPLDKNRYLQFTHCFVEDEEGFCWISTNHGL
                     FKTSIADMIDAYENDLPRVYYHYYGLNDGMVMTEMNGGCLPCAITLRNNTISFPTMDG
                     LLWVNPKEAIPALPDGEIFADEVMVDNKRVESSLFGQTPLSPGKHDIHIRLGFSAWCN
                     KENVYLEYQLGDDDSWRPIDTDNGAVFSFNNLAPGNYN"
     assembly_gap    10524..10540
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10541..12064)
                     /locus_tag="PK977_00385"
     CDS             complement(<10541..12064)
                     /locus_tag="PK977_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16581.1"
                     /translation="MALRCSIITLLLTCYFAAVGQTEDYRIAHYGTEDGLPSNGIKGM
                     QWDRQTGFLWVATEAGLVRYNGMTFKTFTNDDNKQITNERMLFLVKNNTGRIYTADNT
                     GNIFSVQKNNLSFYEKISISGNSKNNAISLSVSETLFKKKHQFVNGPFTLQFDRVLPV
                     NDTLCIINRYDKVFYFSISVASPVLLPFPIKQLRSSFMCRGRVFTCDENNNIWLIDPF
                     NKSLGKTKIEFRTANVQKPLLTKSFLAWENGMEYPIVIDGANAWKISYENEKLIATNI
                     CTAVPQDALIRYVQYDEQRGTLFLGTDSKGIIIIRKNKITQLRKKRTAVDERTSYYSQ
                     LELPDGTVMTNEGHVLGKKDQEYRTSTGIQKFSISTYLMGDSLFWFTAQNMGSGGSRL
                     NCYNLKTKTLKRFDKIRESYSQLVMTSVNGILYVANELGISRLEGDSLKMLFSYSKTE
                     KPQIHFDIKETEPGILYIANCNALLRYDIAKNKTEKIYLSGNYCVRTIWQYKEYLFFG
                     "
     gene            complement(12098..12706)
                     /locus_tag="PK977_00390"
     CDS             complement(12098..12706)
                     /locus_tag="PK977_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF16582.1"
                     /translation="MHPQILLADDHSMIRKGLKLYLQLNLGYSDIQEVDSCGGLMKEL
                     VKKRYTHLILDIILSDGSTLEVIPNIRRVYPDLSIMIFSMQPAEIYGEAVKQFGINYY
                     LSKSAGEEEIIQVLKKFIQNEAPVRKTVLPHHDNPFKSLAPRELEILHYVLKGIGTKE
                     ISETLNLKMNTVSTLKSRIYEKTNAGNIKELLELATLYNVNY"
     gene            complement(12719..>13361)
                     /locus_tag="PK977_00395"
     CDS             complement(12719..>13361)
                     /locus_tag="PK977_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF16583.1"
                     /translation="GKQVPPFNRPFTNSGNYTVSLVITDTSCCGSPPFTQNISINSAP
                     SVNAGPDKYISVNTSTTLDATVSPAGSYSYLWTPGNYLSSVTVLNPVSTPAFTPVTYT
                     LEVTDRTTFCTGKDAVTIIPVSQLYIPTAFTPNNDGRNDKWNIPGLALYPDAVVTVYN
                     RWGEKIFESKNYINNPWDGKYKGIQQSGDTFVYTIVLNDAKKQFFKGTVTIIK"
     assembly_gap    13362..13496
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13497..>13889)
                     /locus_tag="PK977_00400"
     CDS             complement(<13497..>13889)
                     /locus_tag="PK977_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF16584.1"
                     /translation="DDGSTQNTKDARKRFATTGNHDVRYRVYADNGCIGDTIKTINII
                     NGTGPQLSFTITGNRCKDSVLTFTSSITPNASNPTTWYWDFGGGQTVTSSTSNSTTHS
                     FPAAGTNIPVRHTTTFTTGCQPDTITVNI"
     assembly_gap    13890..13899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13900..15837)
                     /locus_tag="PK977_00405"
     CDS             complement(<13900..15837)
                     /locus_tag="PK977_00405"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HRF16585.1"
                     /translation="MHFRGLTIFIILLFWHTTAYQQNISNKGKEFWLGYGYNSWFFIP
                     DGALPPNSQELNLYLTTEAAATVTVSIPNTGWSQTLNIPANTVDATILIPKSGTNDAR
                     ITAEGLNNKAIHIISDTPIVVFAHMYNTQTSGATMLIPVEAFGYKYYSLNYSQSQSNS
                     RVPYSYSNTTANGTNWYSWFYVVASEDNTRLEITPSDTTQSGVLPGQTFTVNLNRGDV
                     YNVMGKLVSGGNQLWQASKDLTGSKVVSVPGNDGNCHPFALFSGSGGIRFCYFDGGEV
                     MMQQAFPTQAWGTRYLTYHMINNTNTDINDPFKNFYRIAVDDPTTVVRRNGVPMTGLI
                     NNFYYEIIDSIGGDYITADKPIMVAQYTPGGNRCWNNSQFAYGDPEMIYLSPIEQGSK
                     DALFYANRKTFIDFNYLNVLVQSTGIPSLRIDGNPFDPVNIITHPNNPSYSVAIARIT
                     GAAGQHRLTCDSVFNATIYGLGFFESYGYNVGTFVNNLNSYSQIQNTLNTNGKIDTFT
                     CPKTPVRLYAKLAHAANSITWKLSQVPGISPNADSVVANPPLVRTEVINGRTYYVYTL
                     QQDFIFNAPGTYKIPIAYSSTVIENCNQTENVELTVLVKPGPPADFTISNQLCLSDTV
                     RFTGTTSTVNGLFNYTSYLWNF"
     gene            15965..>17846
                     /locus_tag="PK977_00410"
     CDS             15965..>17846
                     /locus_tag="PK977_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16586.1"
                     /translation="MNKSCFGKLPASLLAILFLLFTMVAAGQGFYNTTNWRFSNPKQF
                     GFAVIDMDFFDNNNGIAVGANGGIAYTKNGGTTWSYGNFSFINQSGLQTSTSFQDVHF
                     ISATTAYAVGSNGCMAKTTDGGVTWNRVNNPLFANARNVNTVWFLNENKGYIGGQHNN
                     TPDLTPKLYFTLNGGATWDSISAPVGGKTRVGYVNNPVGSFLWDINAKDKELYRIIFW
                     DDNTGYICGSGLSTFEPIPNVSSTSPCNPTGTNTSTGSHHASLLWKFSNGTLTDYSIS
                     KERLGYNGIYTTLPGACNYRYASNSIHTQTYKAMHIIDANNVLLVSLNNNIVVRVNTA
                     PGAITPNINAPGVNEIGTYQLLNAPTPPQNNSSALGAPIPANPAFGFSQPTNIVKAAN
                     GKLFTNVNSPVLFPVNRMMTSADNGATWREERWLPTGRNYSEFGGQAIDILPSGKFVA
                     AGQNGVISDSIPGGQWNSTYVQGTTGSFNKMDVADCNNIIAAGGGTIARTRDGGKTWD
                     QVVRQDFINLNISINSVAYATGNPAKAYFATSVGNIYRTTDMNVAPPALPTLYILCRV
                     SKCGMLPHRGMIVYGYAVTLQLLLQRRFQEYFVQLMVEIHGHKQQHFLPIQVQQIIYS
                     GIL"
     assembly_gap    17847..18029
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18030..19137
                     /locus_tag="PK977_00415"
     CDS             <18030..19137
                     /locus_tag="PK977_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF16587.1"
                     /translation="VFLAGNGFPRKAIFRTTDGGNTWQDITGNALAIFPVGNFNSVVF
                     HDINNGYVGCAGGFLVTSNGGVTWRIDQTASPTNHTSIAFAPKKVPAGTPAANRRLFS
                     VGVFSNHILEYGDTTNLNVSSSELMVSSCNNTANGSITVIAAGGIPPYTYSIDGGAFQ
                     STGTFNNVSAGNHTITIRDFACGLITKTVIVPVRPAPSVSAGPDKTIVEGDEVMLQGS
                     SSGNPATIVWAPNNTILSAGNTLTPIVRPLTSTTYTMTVTDANGCISADNAQVTVLPY
                     CLKVMNAFTPNGDGTNDRWIVTNNGGLCVTNVYVTVFNRYGGEVYVNPNYQNNWDGTY
                     KGSPVPDATYYYVITYTLFNGRKVTVKGDVTILR"
     gene            19157..>19384
                     /locus_tag="PK977_00420"
     CDS             19157..>19384
                     /locus_tag="PK977_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF16588.1"
                     /translation="MKKYQFILVCLLAAVVSGKAQQLQTSSMYDLQGVYHNPSTAGTQ
                     GFNFVGASYRTQWSGFSGSPKTATVFGSFELP"
     assembly_gap    19385..19442
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19443..>19965
                     /locus_tag="PK977_00425"
     CDS             <19443..>19965
                     /locus_tag="PK977_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF16589.1"
                     /translation="TSRTGIQLAFAKHIPLNNGGKFSLGIEARGLQFAIDRAKLSETL
                     GADPALGTSENRFKFDAGFGISYNGKKLQLGVSASQLIQSKLDFYSGNLTPTEAGRLY
                     RHYYFHGAYRWNVDGATTITPNALVIYLPNAPATYQLGARVEHNEVFWWGLGYRVDQS
                     FILSAGVNVHKKFT"
     assembly_gap    19966..20039
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20187..20987
                     /locus_tag="PK977_00430"
     CDS             20187..20987
                     /locus_tag="PK977_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF16590.1"
                     /translation="MSNLLVTVIQTNLHWEDKKANLQMLEEKIGNIKERTEVVVLPEM
                     FSTGFSMKPEQLAETMEEETVQWMKRMAAEKKIILTGSVIIKEKENYYNRLIWMLPNG
                     QYGVYDKRHRFAFAGEDKHYTAGTKRLIASVKGWKINLQVCYDLRFPVWARQQSQPDG
                     PEYDILIYVANWPERRSHAWKTLLQARAIENQCYVIGVNRVGNDGNAIYHSGDSMIID
                     PMGEVLYHKKDEEDVFTISFDKKHLETIREKLPFLRDADGFMILNEAE"
     gene            complement(20992..22512)
                     /locus_tag="PK977_00435"
     CDS             complement(20992..22512)
                     /locus_tag="PK977_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF16591.1"
                     /translation="MGFERIMRILLILLLAAGGLAGCGVSKSSFSPYKKYSPAELMED
                     YSVYRQVLEKHHPSLYWYTPKDSMDYYFEYGQHQLKDSMTEPAFRKVLTYVTAKISCG
                     HTTVRPSKAWSRYSDTARLGKMFPLSMKVWDMAMLVNANLNRRDSILKRGTVITRING
                     RTAEAVVDTLFDYISTDGYNRTHKYQSLSNRGNFGSLYTLLFGPADKYDIEFKDSTGT
                     IKTTVVPVYDPATDTAGRTGTRTFRPAQQLTKKQRRELRLNAARLLRIDTVNRTAMMD
                     LATFGKGVGLRRFFHNSFEALEKNKIGHLIIDVRGNGGGSVTNSTLITRYLAAQKFKI
                     SDSLFAIRRGSGYERYVEHHFWNKLFMLFFTRKKKDGNYHFGYFERHYFKPKKRHHYD
                     GKVYIVTGGNSFSATTLFASALVKQDNITVVGEETGGGAYGNSAWLIPDVTLPVTGIR
                     FRLPLFRLVIDKTTPKNGRGVQPEIESFPTIDAIKRNADFKVEKVMELIKIDKAER"
     gene            complement(22512..23555)
                     /gene="thiL"
                     /locus_tag="PK977_00440"
     CDS             complement(22512..23555)
                     /gene="thiL"
                     /locus_tag="PK977_00440"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRF16592.1"
                     /translation="MSKERTEISSLGEFGLIDHLTKNIELQNASSVLGVGDDAAVIDH
                     FGKQTVITTDLLLEGVHFDLVYTPLKHLGYKSVIVNLSDVYAMNAIPTQVIMSIGISN
                     RFSVEAIDEFYEGVYAACNKYGVDLVGGDTTSSQKGLVISVTAIGEVTPDTYVKRSTA
                     KKGDLLCVSGDLGAAYVGLLFLEREKKIYMESPGVQPDLEGESYVIGRLLKPEARKDI
                     VEFFAKEEIQPTAMIDISDGLSSEILHICKESALGCVLYEEKIPIAEEMKKAAFKFEI
                     DPTACALSGGEDYELLFTIAQSDYEKLVLNEQISVVGYMTEPDQGAHIITKGGGKHAI
                     TAHGWNHLKQNNS"
     gene            complement(23552..24331)
                     /locus_tag="PK977_00445"
     CDS             complement(23552..24331)
                     /locus_tag="PK977_00445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRF16593.1"
                     /translation="MLKTTLTEAVRAGAAEILRFFNRDFIISNKEGVNNLVTEADHAS
                     EKAILDVIRKNYPDHHFLTEETGEIIADSQYKWIIDPIDGTVNFAHGIPLNCVSIGIE
                     HNGEMVMGAVYNPHMNEFFFAEKGKGATLNDKSIKVSNETQAIRACLVTGFPYTYINM
                     PNGPLEIFDRFVRKGVPVRRLGSAAIDLCWVACGRFDGFYEHKLEPWDSAAGYLIVEE
                     AGGKVTDHQGNKFSVYQHKVLATNGKIHDEMVAVINNQKEL"
     gene            24408..26354
                     /locus_tag="PK977_00450"
     CDS             24408..26354
                     /locus_tag="PK977_00450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF16594.1"
                     /translation="MQLSVIIVNYNVRYFLEQCLYSVQKACRGLKAEVIVVDNNSADN
                     SVEFLSSGFPSVQFLINNENLGFARACNQGWQRAKGEYILFLNPDTIVPEDGFEKCLS
                     FFQTNPDAGALGVKMLDGSGKFLKESKRAFPSPLTSLYKLCGLAKLFPRSRTFARYHL
                     GHLDENKNNEVDVLAGAFMMIRREVLEKVGSFDETFFMYGEDIDLSYRIQQAGYKNYY
                     FAGTSIIHFKGESTRKGSMNYVRMFYRAMSVFVKKHYGGSRAGVFNFLIQTAIWFRAL
                     LTAMGTFIRRAGLPLIDAGLILLSFWLMKNVWNEYVRTDIHYENKLLWIAFPAFTIFY
                     LISAYYAGLYDRWYKRSELVRSTLIATLVLLAAYALLPEQYRFSRAIILFGALLAFVL
                     ISLLRWLLVKTEVLNSDKEKEDRYTTVIAGNADEYRQTMELLQVAGLQQRILGRVDPA
                     GSDANSLGSVNKLTQLTSTVPFREIIFCEGKLSFADIIKNMQQLPRGTIAKIHAEGSQ
                     SIVGSCSSNTAGETVTKENGFKLADPYNRRLKRLIDTSLALLALITFPVHLFLVKKPG
                     SFLANCFTVLLAQKTWVGYAIPDKSLPQLKPSVLASNGIPATTTQQLPKESLQMMDYW
                     YARDYEPATDLKIIRKMYRKLGGE"
     gene            26430..27962
                     /locus_tag="PK977_00455"
     CDS             26430..27962
                     /locus_tag="PK977_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF16595.1"
                     /translation="MATIIDIKLPRALAAALRIPPNDPRRQQIRVLKKLLKKARFTEF
                     GQQYRFDEALLSKHPGRKFQELVPVHDYNKIYEEWWKKTLDGVPDVAWPGKIKYYALS
                     SGTSEAASKYIPVTRDMLRSNTVNYLKQLFSLMRYEEANKKAMTKGFLMIGGATDLKK
                     GKAGWYAGDLSGILAKKRPFWFQSFYKPGGRIAAMSDWNQKLHEIVEHAKEWDIGYIV
                     GVPAWCQMCMEMVIEHYKVKTIHDIWPNFSFFVHGGVAFEPYKKGFEKLLAKPIVYIE
                     NYLSSEGFIGFKTREHAKGMKLILDNNIFFEFVPFDDKNFDHDGNIIASPEAKMIHEV
                     EEGKEYALLMSTNSGAWRYLIGDTIKFVDLERCEVVITGRTKHFLSLTGEHLSVENMN
                     KAIELVSEELNVSIPEYTVVGFPHENMFAHRWYVATNDKVDAEQLCKRIDEHLRQLND
                     DYATERDSALKEVFLEVLPEEKFLQFMEQKGKLGSQHKFPRVMKGKMLEQWNRFLQTG
                     HI"
     gene            28008..28640
                     /locus_tag="PK977_00460"
     CDS             28008..28640
                     /locus_tag="PK977_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRF16596.1"
                     /translation="MLEAVIKGLGFGLLLSISVGPVLFSIIKQSLNNGHKGGFAFVAG
                     VSASDISLVLLSNIFTTLLESLKLYKTEIGIAGCIFLVSMGIFFLFFKKVKVNKEGEQ
                     VFKFRKRDYAKMFLSGYLMNTLNPAIFIFWITTSTAIIGHTVNERIVIFTTCLLFMLL
                     TDCLKVLLAGKIRRKLTPHNIHIINRVNGIILIVFGIALIWGLIFYGKKL"
     gene            28624..29352
                     /gene="mtgA"
                     /locus_tag="PK977_00465"
     CDS             28624..29352
                     /gene="mtgA"
                     /locus_tag="PK977_00465"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRF16597.1"
                     /translation="MAKNSDNRTKNFLRGLWKWTKRIFLWLFIFQLFYIILLKWVNPP
                     ITVTQLVSWVSGHGLKRDYVRSSSISPNAKLAVIAAEDQLFPDHSGFDWKSIKKAMKY
                     NEKKPGRIRGASTISQQVAKNVFLWQGRSWLRKGLEVYFTFMIELIWGKKRILEMYLN
                     VAEMGEGVFGIEMAANKYFNKPAKNLTRQEAAMIAACLPNPKRYTVKPLSAYVSSRSQ
                     FVMRQMNNLQTDPDIQKLIGLSPP"
     gene            complement(29353..31020)
                     /locus_tag="PK977_00470"
     CDS             complement(29353..31020)
                     /locus_tag="PK977_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF16598.1"
                     /translation="MKQLLAKHHRLLFYGLWLLLGLLQARLTELQDDEAYYWVFSRHL
                     DWGYFDHPPMTALLVKAGYAIFPNELGVRFFPLLLNILSLAIIERLTENKNPFLFYAI
                     ALSMAVIQLTGFVAVPDIPLIFFTALFFWCYKKFAAVQSVLNTFLLGLSIAFLLYSKY
                     HAVLIVFFTLLSNLKLFTKYQLYVAGIFSLLLFTPHLWWQYQHDWVSFRYHLFESNVN
                     AYRFSFTSEYVLGQLLIAGPVAGFILLPAAFLYKTKTKTERALCFTLIGIYVFFLLSS
                     FRGKVEGNWTSPALVAVIILAHQFLNEQLQWQKWLYRLLPVTLLLVLSGRVVMIADIL
                     PVKAIRQRYHAWKKWPQEMREKTKGLPVVFSNSYQRASKYWFYSGQTTYSLNLYKERR
                     NNYNFWPIEDSLLGKPVYFLDIWDLWRFPDSMKTPIGFVGYKYDSSFISFSKINITPP
                     TRKIRIKENEAVNLTCSIIAPEHYKRFINGSASASDTIRIGIYKAGKWQRDIFTGIVL
                     KEAVEQKNFSITINPALSPGKYFLRFAINHQTYPSTHNSEKIELVVD"
     gene            complement(31046..31603)
                     /locus_tag="PK977_00475"
     CDS             complement(31046..31603)
                     /locus_tag="PK977_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF16599.1"
                     /translation="MLKKIVIIGPESTGKSTLCEQLAQHYETAWCPEFAREYLLTNGT
                     NYEFEDLLTIAKGQLALEDEYTITLENQSQTYLENGGHLPLFIDTDMYVMKVWCEFVF
                     NKCHRFILDQIVERKYDLYLLCNTDLPWVDDELREYPDLHTRKKLYAIYKDLMINQAV
                     PWVDISGNYEQRLQRAVSAVDTLLK"
     gene            31718..32278
                     /locus_tag="PK977_00480"
     CDS             31718..32278
                     /locus_tag="PK977_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF16600.1"
                     /translation="MHVAMAGEKQNIIQAVKDYGRQLFGFIRNRVSSQEDAEDILQDV
                     WYQFSAQTKSEDIVSISGWLYRVARNRISDSYKRKKENSLEDMMAEDEEGIWQVPEFM
                     TAESIDPETLQLQKMFREELVQALDELPDNQRRVFIWNELEDMTLQQIAQREKENIKT
                     IISRKRYAVQYLRKRLDYLNNEFFNE"
     gene            32297..32638
                     /locus_tag="PK977_00485"
     CDS             32297..32638
                     /locus_tag="PK977_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16601.1"
                     /translation="MKSVIRRKPYLIPLFMILGFGAVALFGWIVMLLWNAALVPVTGA
                     GIISFWQALGLLILSRILVGGFGGGKGRGSRMGARWSHMTPEEKERFKEYCRSRWGKA
                     NPEEPPFTEVQ"
     gene            complement(32655..33287)
                     /gene="pnuC"
                     /locus_tag="PK977_00490"
     CDS             complement(32655..33287)
                     /gene="pnuC"
                     /locus_tag="PK977_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRF16602.1"
                     /translation="MDLQEIWHQFIEGLQKTSLLEYIAVFAGIASVWFSRKENILVYP
                     VGLINTIIYIWLSFKGDLIGEASVNFYYTVMSIYGWVLWTKKDKEKHPVVHISFSDRK
                     WWLYQLAFFVAFYITIFLLLTYLKKDFAPDAIPWADAFASATAFTGMWLMTKKKVESW
                     YWWVATNIASMPLYFVKHYVFTSVYYLILLFMAVWGLFEWSSKAKKQQAF"
     gene            complement(33368..34705)
                     /locus_tag="PK977_00495"
     CDS             complement(33368..34705)
                     /locus_tag="PK977_00495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF16603.1"
                     /translation="MTTTSKLNKFFVHVLFIVASIMSLTLLLSCKKSQDIDTEKNHVS
                     SYSSEVLDKWMTLQLRLIKNATGVSNHGFARHFAYTGIAAFESLVPGLTSTEKKWSAK
                     WNGLTGLPATLKSKNYYYPANVNAAMAAINRSFFPNANATDKAAIDSLESALTQQFLL
                     TRSNNEINVSAQFGKDVAAAVFAWSETDGYKNASAPYTVPTGPGLWKPTPPANTAPAT
                     PYWGNNRTIVAGSTTNTQLNAPIAYSTDPSSPFYQMVKQVYDASQVVTDDQKAMAMYW
                     RDIPGVSSPGHWLSILQQVVSKEKAKLDKTALAYALTGAAVNDALITCFQTKYQYNLV
                     RPITFIREVMGYSNWSTLVGTPAHPEYSSAHASLSAAAADVMTKLFGSPQTFTDHTYD
                     YLNMSPRTYLSFYAIGEEAGMSRFYAGIHYLPSITLGLEQGRKVTANIFLQNH"
     gene            complement(34733..35338)
                     /locus_tag="PK977_00500"
     CDS             complement(34733..35338)
                     /locus_tag="PK977_00500"
                     /inference="COORDINATES: protein motif:HMM:NF023363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HRF16604.1"
                     /translation="MKKQNIIKMRSFLTMIFFAAIITSCKKDKVDTNPTAHKINQSEN
                     LVIPGVIDLPSNLPAGNSRVATYYAEGVQKYKSQAKAGSPGVFEWVFVAPLADLYDVT
                     GKKIGTHAAGPHWTLSPADSIFAQHFSPAKTAPSPDINSVDWLLLMPKSGKIPTGFFK
                     DVAYIQRIATSGGKAPATAPMSESATVEVPYTAIYRFTKKN"
     gene            complement(35382..36227)
                     /locus_tag="PK977_00505"
     CDS             complement(35382..36227)
                     /locus_tag="PK977_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF16605.1"
                     /translation="MEKQLELIREQQRASWNKFSPGWKKWDELFMDFLKPMGDEIIHQ
                     LNPENDDVVLDIASGTGEPGLSIANLVKNGKVVITDLAEGMVDIARENALLRGINNIE
                     TLTCDVSDIPFLDNSFDAISCRFGFMFFPDMLLAAKEMHRVLKPGGRIATSVWNVPEK
                     NFWITAMMGTINQHMELPASPPGSPGMFRAAKEGLMADIFKQAGLKNISVTEVKSQMN
                     CGTTDLYWTVMNEVAAPVVAALSNTDEFLKEKIKREVFDVVNRKYPDQYLKIDASSLI
                     IYGEK"
     gene            36602..40189
                     /locus_tag="PK977_00510"
     CDS             36602..40189
                     /locus_tag="PK977_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF16606.1"
                     /translation="MLNEKSFTRYATGDGLSDNTVTGIEQDAAGYIWLATFSGLNRYD
                     GSRFIKYHSTNDSLSPATENFVRINRLDKDRIAFLPMGLHVVNTKTNERRNLFIPYHN
                     KQYQYKYNMVERAAGDDKGNLFVLTRSGFYHYDKNYRLLFRFDYFSEKDLPTEHFYFG
                     NDLLELDDKRLLITARPGLYIYNKEKKEFLKLSPGQLPLLDEFTHDNTYYKFFQVKRG
                     KLLVTKSESDTIFYIDISVNKKIVSRSPFTFSNDIIQWRSKLIPVNDSLFYITGQTSG
                     FYSLKLNPSSGSVQFMPEKYFADYLCNDLLTDKENRLWVATNNGLFRQNDNRHQVQTT
                     YISDELKAKFPNLSLTSIYVTSDKIYAGARAGAGLLVFDKKTFRFENSWFNGSDVKNN
                     TVFKIVSPYNSSLIIGSLGPISLYNNTNGSIKTMHPPGWNPNDWAGDLLKDSRGDIWV
                     SSSNIYRYRPAEKKFILIPFSPSMPSVPVAIAEDRNGNIWMAGHGIIRYNVSYSRFDE
                     KIDSFPYIKMPDKQVGPLAIDRDNNIWFGSANNGLIGYNIDKKLYRHFTTGNGLPGND
                     ISALIIIGNKLWIACYAGLACLDLTSLQIKRFGKDEGFPEMPLQNRAGFFYDSSQQQL
                     YIGFSTALVRFNPYEILTPQKQPSVFIENMDIGNKKIYFPGRIVSATWKQNDLKLTIG
                     VINFNDGRSQGYAYRIFKNSNSPWQQLGNQPSFSVSNLSPGKHRIQVKVFSLNNQWPE
                     QVKEIIVEVLPPFWQKDWFRIMATLLSLALIFLFIQWRIAVARKKEMEKTHVQKLKAD
                     DYKNKYELEQITNYFSSSLADKKSADEVLWDVAENLIGRLNYLDCMIYLWNNDKTKMI
                     QKAAYGLKAKPELLSEQVFDVKSGQGVVGYVMQTKEPVLIKDTRTDSRYRVDEVRRLS
                     EICVPIIHNNELLGIIDSEHPELNYYSERDLKILTTIATLIGNKLKQLESEQTLEVKE
                     QELSNINEQLAEAKLSALQAQMNPHFVFNALNSIKRMILDGDDDKASRYLSKFAHMIR
                     MTLNHSKETFVTIAENIEYIKTYLGMEQLRFNDSFKWTITIADNIDADETRIASLMIQ
                     PLVENAIWHGLLHSEKEKILSISFTRNEDTITCIIEDNGIGIRKSQKAKEQHQSNHRS
                     VGLDNLYNRIKILNEKFNAGYSLIITDCSENNENCTGTRVTLKFIINDN"
     gene            40200..>40800
                     /locus_tag="PK977_00515"
     CDS             40200..>40800
                     /locus_tag="PK977_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF16607.1"
                     /translation="MKAILVDDEPDGVKALQMMLGRHCPSVDIAAVCNNADTAKQQIE
                     AIKPDVVFLDIQMPGKSGIEMIGELPEKKFEIIFVTAHNEYMLQALQYSAADYLLKPV
                     DEDRLAEAVQRVEKRLLEGKKEELTETLLYNIGKAGQPSEMRLCLPTLKGFIILKLDD
                     IIYCEAERSYSVFHIEGRKPVTVSKPLIEYENMLTNTSFF"
BASE COUNT        11709 a         8292 c         8689 g        11606 t
ORIGIN      
        1 ttaataattt ctccagtggt tcttttttta caaacggggt atcgccctgc acattcacaa
       61 taatatcaac atccatctct gctgccgctt cagcaatacg gtcgctgccg ctttcatgtt
      121 catgctgact catcaccgct ttgccgcctg ctgctgttat ttctgtatag ataatatcac
      181 tgtcggtcac gacaattact tcatcaaaca aacctgtggc cacagtattt tcatacgtat
      241 gccgaatgac agacttgccc cctaacagtt gcattagttt accgggaaat cttgttgctg
      301 catacctggc aggaataaaa gcgaatttct tcatgcttca aatatcctca taatcctcca
      361 ctcaataaaa ctaccaactg gttttcctgc atatacattt caaacacata agaagcgcct
      421 tcatcatagc tgatcctgat tttctcaatg gtaaaaggtc cgccgtattc cgggtcgtca
      481 accgttttga aaagtttgat atcatattct cttccgtctt tggttatggt tttgctggca
      541 gtgggaaaca catctttttc tccaaacaca tctttgaatt caggaattaa ccttaacaac
      601 agaaaaccct gctgcaacga aaagccggga agaaagtaag tatcagaagc atattcatac
      661 gctaagaaat catggtgctc tgcgccgttc ttgtacaatt gcttgctcag ctgccatccg
      721 ctttccggaa tctggggtgt tgccccgctt cgggtcagca gtttttgtcc aaagggaaca
      781 gatacctgtg agaggtattg cttcatgtct tttaccgttg ccagttttgg cggaggccag
      841 ggaagcaaat aactgtctac gatgtagcct gtcttgttat tgtattttac ttttctccag
      901 tagcctgtta attcttcggt gatgatggtt gtccagccac catcttcatc aataaggttc
      961 agttttgttc cgtaaggaat tttgtccagc accttagatg cagggtcagg tttttcccgg
     1021 atgctcagtc ctgttttcgc cgctacatat aaaggatcgt tttggctgtg cagtgtgaag
     1081 gatataaaaa cagccaatgc agtaataagc agtttcatag aaaacaatta agatggttta
     1141 tgatttggta aatggtttct ttaatagcag caatacatcc tcatctcttt cttccggaaa
     1201 atcatggtcg ataccgtaac gaacttcttt aatgttcagc cggctgaagg tgcccagcag
     1261 gcggtcccag atggaaaata cagcgccgta gttggtatcg gtgtagggct gcttccagtg
     1321 gtggtgcact ttgtgcatat tgggagagat aagaaaataa ctcagtgcct tatccacttt
     1381 ttccggcagg ctgatattgg catgtgtaaa agcggtagcc agtaccacca gtgtctggta
     1441 gatcatcacg ccgtacatcg gcgcaccgga aacaaagatc agcagcataa aaaagatgcc
     1501 ccgcagcagg ctgtcaccgg gatgatggcg aagaccggtg gtaacatcta cgttggtatc
     1561 gctgtgatga ataacatgaa aacgccatag aaatttattc ttgtgcatca cgaggtgcac
     1621 cagccagctg ctgaagtcaa gggcaaatac accaataagc accgttgcca gcacaccggc
     1681 atcgaaccag tatactaaac cgaattgatt gtgcaggcac cagtcagata atttcacaat
     1741 gaaaaaagca agaccggtgt gcaggattaa atgcagcacg gtcagtacga aattcacgcc
     1801 ggcatgacgg attttgtttt ttcggtaatg aggtctgaaa agaggaatag caccttccag
     1861 tatccagaag aaaagtaatc cgccgacaag gatagccatt cgttcgagcg gccgttgttc
     1921 aagggtttga aaatgtgcaa tgatctgttc ccacatggca ataagattat tggtaaaagg
     1981 tacgaaggaa taaaataaag aagatcagtt gttggcagca gattcgttat cctctttctt
     2041 ttcatcaaca ggaggccatt catgcttgca atcaaaacaa tgatagacag attcaatggc
     2101 gggtgcatag ctggtggcaa atatcccgat cagcacactg agccagtttg aagcttttcg
     2161 gggagtgctt accagttcta tattgtgcga accgcatttg gggcaggcat gtgcagcttt
     2221 gctttccctg cgcaggcgat ccaatatttc cctggcccgt tccgattgtg atttggcgac
     2281 catcagcttg ataccgccca cagcattggt caatatcggg tcgatggtaa ccgtgttctc
     2341 atctttcaac cagcagttga taccttcttc ctccagcaca cctcttgcaa tgtgtgcttc
     2401 tacatagttt gtatagctgc ttaataaaac aaactccata gtacatgttt aacgtccata
     2461 ctcatctcca tattcttctg catatttttc ataccgcttg atcatctcaa tgtttttctt
     2521 ttcaatatcg gtcaggaagc cccggatatc aacagtgttg ggcacatacc attcttccat
     2581 ttcaaattgc tggcgcaaat gttttttgct gaagcaataa ccatgacggg caaagatctc
     2641 gttgcgcata aactcgagct cttctttcat caggttctct acatcttccg gcttcagcaa
     2701 acgctgtgaa gactcaggcc atgtgcctac atcggtacgg tattcaaatt tttttcgttg
     2761 caactggtat tctttttcgg cacggccttt tgcataaggc ttccagccgc cgtttatatg
     2821 gtcggggctg gtatcatcca ttacccagtt aaactctccg tcatctttat gatctcccgg
     2881 ttctttggca ttaaaataat agaaatcacc ctctttctta aatgtgccgc ggaacggccg
     2941 gtcgttgcca ccaacaatgg tgcggcccac tacagaatca cctgctatta aggttatcag
     3001 catggtgatt ttattggtgc cgaatggccc gacaaaagaa cccaataatt tctcgtaagg
     3061 cttaacggca acctgcagtg gtgtttgctt taattctgtt gtgccggcct gggtggtggt
     3121 gtcggtcttg tcttcctgct tgtctgcagg agcatctgat tttttatcat tattgcaggc
     3181 aacaaaaagg aacccggcga taaataagca gctaattttt ctcatggtga aaggtttaaa
     3241 tttggtgaaa gcattactaa ctaaatataa aacaaattct ttcccgtaca aacatatgga
     3301 acgcaggcct gctgattatt ctggctttct ttacctggct gatggctgga ataactattg
     3361 attatattcc ttacaatact gatgtcggct tcctgcgaat taaacagcag tacatcgaga
     3421 ttgaccactg gcggattgct ttctttattc atgtatatgc cagcatctgg gtattgctgg
     3481 caggcttcac tcaattttct ggttgggtgc aacgaaacaa tccgaagctg caccgggcca
     3541 tgggctatat ctatgtggca gatgttttac tggtcactgg tcctgccggt ttgctgatgg
     3601 gtttttacgc caacggcggc ttgctgtcaa gaatcgcttt tgtgttgctg gcgtttttat
     3661 ggatatactt cacggccatg gcattgatca aagcaaaaca aaaagatttc aaatcccatc
     3721 gtcattatat gatccgcagt tatgccctca ccttatcggc gctcacatta agggcatgga
     3781 aatatgtaat caccaacgca taccatattg atcccaactg ggtctttccg ccgatggatg
     3841 tataccgggt ggttgcatgg ctgggctggg tggtgaacct gggagtggca gagtatattg
     3901 tttaccgcag taaactaaaa agcttttata aaagaagaag cctggagcag ggataggtta
     3961 attagtcaat aaggagtttg tgcttttaat aaaaaagctc acaaacattg tgagcttcgg
     4021 ttatcttatt aatcgtggtt cttctactga ttcagcatca gcggcattac cagcatcagc
     4081 aattcttcat tctcatcctg gtcagttggt ttaatgatgc cggccttggt aggtgttgac
     4141 agctcaatat ttacttcatc actttccgta gcattcagca tttcaatcag gaaacgggca
     4201 ttaaaggcta tcatcaggtc ttcgccattg tactggcact tcattctttc atttccttca
     4261 aagctgaaat ccacatcctg tgcagccagt tgcaattcgc tgccgctgat actcaatgct
     4321 acctggttgg tgcttttatt actgaataca cttacacggc gcaatgcact ttggaaatcg
     4381 cttttattca cagtcagctt atacgggttg tctgccggta ttaccacctt gtaatcaggg
     4441 aaacgggcat caatcaggcg gcaactcatt tgggtagtgc cgtgttttac aaacaggtgg
     4501 ttgctgttat agcttacggt tatttcatct tcattggaag gaatcgctgt tttcagcagt
     4561 gtcaaaggtt tgcggggcgc aatgaatgaa tctgttttag gacattttac atccgttctc
     4621 ttataacgaa ccagcctgtg tgcatcagtg gccacacact gcaatccttt tttatccagt
     4681 tcgaagaaaa caccggtcat ggccggacgc aaatcatcgt tgctcgtagc aaaaatcgtt
     4741 ttgttgatgg cggttaccag tgctgaagcg gtcatggtga acgaagtggt atcatccgca
     4801 cccggttctt tggggaagtt atccgggttt tcacccatta ctttatactt accgttgtcg
     4861 ctggtgatct caataccaaa attcttgtca atattaaatg tgagcggctg gtcgggcagg
     4921 ttcttcagtg aatccatcag tattttggca gggatacaca ctttgccgct gtctttggcc
     4981 tcaatatcca gctgaaccct cattacggtt tccaggtcgg tagccactac ggtcagctta
     5041 tttttttcca cttcaaacaa aaaatcttcc agtatcggca ataccgtgtt ggcgttgatc
     5101 acaccggcga tatgctgcag ttgctttaat aaagatgagg aggatgctat gaacttcatc
     5161 gtattcttgg tttgtggcga aactactgat ttttggttaa cagccaacac tttagacacc
     5221 ggcgtggatt actggctaag ggctgtggaa aggggttttc ccggcttgtt ccttttccct
     5281 tcttttgtat cttgcttact ctcaactaca cagcatgttt gataaacttt ttggctgggg
     5341 aaaaaagaag gcagggcaag aggcagaacc tgaaatttcc tttggccgct attcagataa
     5401 taacaagcca ctggcaaagg tgaaccgctg gacggatgcc gataatttgt tcaaggaaaa
     5461 aaagtatcct gaaagccttg atgctttttt tgaatacctg cgggatgata ctattcagaa
     5521 tgtggtgtac gaacggaatg gtgcagaggg ccggtttctt ttttaccagg gctcaaaaat
     5581 tgtaaggggc agttttgatg gggagctgtt gaaagcggaa gttacactgg cccgtatgcc
     5641 gcagcccagc gtaccggtga tgcgccgctt gcttgaaatg aatttcaatt tgtactatag
     5701 ccggttttct ttggacaatg atcgtctttg tatgcgtttt gacagcgatg ctgaaacggc
     5761 cagcccaagc aaattgtatt atgggttgaa agaactggca acaaaagcgg ataaacaaga
     5821 cgacctgctg gtgcaggact tcactatcct ggaaacaagc gacagtgaac acattacagt
     5881 gttgtcagaa aatgaaaagt tggtgaagta cgagttcctg caaaaatgga tcaaacaaac
     5941 actggacatt atttcaccgc tggatgccga taaattttcc ggtggtatcg cttacctgct
     6001 tttggctttg atctgccgca tcgattattt gataactcca gagggaaaat tgcttcatga
     6061 actggaaaaa attgccggta tttattttaa gaaagatgaa cggccggtgg tggaaaagaa
     6121 ccgggacatg atcgaagagt tcaaaaagat tcaggcaata cccaaagaag aagtgttcaa
     6181 aaacctgttt cgttccaaac acacttttgc tattgtggca ccacagcagt acaaaactat
     6241 cgccgacaat attcatgcat ccaaccagaa catgatgtgg taccgcgaca ataattaccc
     6301 gttcatcgcc acacagattt gcgaatatgg tatttcttat tgccagtatt cgtacagctt
     6361 acccagcgtc attacagagc tattccattt gtttatgatg gtaaactatc ccgattactt
     6421 ttcagcatta ggctataaaa cagcgtacta cgatccggcc agcggcaaat ttgaaaatga
     6481 gtcgataata gaaaaagtat tatccataca gcagaaatgg aaagaaaaat acccgctgat
     6541 ggattttaaa acacagaacc tgcggtttga cagcctggtg aattttgacc tgacatttac
     6601 caatgagatg gaatttttaa atatggaacc caagtaatca atgatatgga tcctcaactg
     6661 ataatagaaa aataccagaa cgctattata caaattgcca ccgctggtgg taccggtacc
     6721 ggattttatg tgaaggagta tgaccttatc gtaaccaacg accatgtggt ggcagataat
     6781 gcagaagtga ccattgccgg aaaaacattt gacaaggcgt tgtcaagggt ttggtacacc
     6841 gaccgcaaac atgaccttgc tttcctggaa gcgccaaaga atattgaatt gccggaggta
     6901 agactaggcc tgtatgaaca aatgaaagat ggtgatgtgg tggtagctat tggtcatcca
     6961 tacggtctga attatacagc tacacaaggg gtcatttcta aagttgaccg gataagagaa
     7021 ggcttgaagt atattcagat tgatgctgcc ataaatcccg gcaatagtgg cgggccattg
     7081 gtgaatatga atggcgaggt catcggtgtt aattcattta ttattcgcgg tggtgataac
     7141 ctgggatttg cattacccgt taattacctg agggaggctt tgcaattata tacgcccaac
     7201 agggggcagg cctcaacccg ctgtttcagc tgtggttacc tggtgactac tcaaaacatt
     7261 gattcaaaaa aatactgtcc aagttgtggt acagaagtaa agctgccgga aatacccgaa
     7321 aaagaagtag aagccattgg cgcagcaaaa acaatagagg agatattaaa agaacttggc
     7381 aaagatgtca aactcgcaag agaaggggtg aataactgga gtgtgaaaga gggatctgct
     7441 aaaatcaaga taacctataa ccccgataat tatttcattg ccggcgacgc ctacctatgc
     7501 cagatgccgt cagacgccac gaagatcaaa ccgctttatc agttcctgtt acaggaaaat
     7561 tatcgcatga acggattggt gcttagttgt gtcaaacaaa atattgtgct aagcagtgta
     7621 atgtatgacc tcgatcttac caaagacaac ggagtagaag cctttagccg tttattccgg
     7681 caggccgatc attacgacga ctttttgaag aaggagtatg gctgtacaga aagactggaa
     7741 gaataacctg acaccacagg aattttcgca tgtacaaaaa atcattgacc aaagaacagg
     7801 ccctgcaaaa gctaaagcat tactgcgcct accaggaacg ctgccatagc gaggtcaaag
     7861 aaaagctata ccagctggga gtatggaaaa aagaccatga tgaaataatt gcctcattaa
     7921 ttgaagaaaa ttatctaaac gaagaacgat ttgccattgc ctatgcaggt ggtaaattca
     7981 gggtgaaaca atggggccgg gtaaaaatta agtatgagct gaaacagaaa caggttagtg
     8041 attacagtat taaaaaagcg atgaaacaga ttgatgagga agaatatctt tctgtattga
     8101 ataagctggc caaagaaaaa tatgctgcgc ttaaaagtga acaatacctg gtgcgtaaaa
     8161 agaaaacgat ggattacctg cttgcaaaag gctttgaatc ggaccttgtt agaaaggcag
     8221 tggagaatga ataactttca tcactcttat ttaccgccat cgttgatagc ataatacttg
     8281 tcttcccatt cctggtaatt cttctgtgca ttttttacaa aaaaggactt gctgattata
     8341 ttgataatgc ccttgttgat agctgccagc ggagttttga acgggcggat aatatcaatc
     8401 agcaatacaa cacgaacctg gttactgttg ttccatactt catgctcata ggtatcatca
     8461 aaaacaacag cctttccttc ctcccaaaga attttttgat tatcaatccg catcaggcaa
     8521 tctcctttct tccccggaat gattaggcca aggtgacttc ttatcaggcc cttgaagacg
     8581 cctttatggg cgggtatatg tttgcccggg tagaggatgg aaaaaaaagc ggttttcata
     8641 tccggaatct tttccagcag ggaagccgta acgggacaag cttcgcaatt cttgactgct
     8701 tttaatccga atccaaataa gaaatatgtt ttccagttat tatcctggtt taaaacaatt
     8761 tgttctttct gtatatcctg caggctcggg aattgctgat ggttagccag aatgccttcc
     8821 agctctttgc ggatcgtcag ccagttttct tccaaaagcg ttgtccattc aaaatcttta
     8881 ttatcaaaaa cagggagatc gccaacaggt gaatatttac ccatccaggc gccaatcttc
     8941 agcagcagcg gcctgaatat cctgaactta ataaattctc ccggtttcat ttgctcattt
     9001 ttatagaaac agtagtgccg gctcctttgg tgctcgtaat atccagtgag gctcccattg
     9061 ttttaaggag atcttttata ataagataac caaggccatg accctttttg ttgtctacgt
     9121 tggcggcagt gattactact tcgtcttcca tcagccgctg tatctgttcc gggctcatac
     9181 caataccttc atctttaact gaaacaacaa tatgaccgtt ttctattctg gtattaacag
     9241 cnnnnnnnnn nnnnnnnngc ctttttcggt gaactgaatg gcgtttgtca gcaggttata
     9301 gatgaggatt tttaacggct cgtaaaactg gttaatttct attgacgggt caacattgtt
     9361 ttcgatgaca aggctttttt ggcgggccag ggtttgtaaa atacccaata cctgtgctac
     9421 catttcattc aggttaaaca cctctttcac cattctccgg ttttcatttt ggtattttat
     9481 ccagttcagg atattggtag acaacaattg cagttcttgc gaagtattgg tcatttcccg
     9541 aacggtctcc tgttgcaaat cttcgctcat gagttttctt ttctccagca ggttttttcc
     9601 ggcagcagtt agaaatttca gaggagtaac aatgtcgtgg ctgatgatag aaataagcct
     9661 tgttttgata ctgttgttct tttctaatat ttcattttgc tcctgtagtt ctttggtttt
     9721 ttcggcaacc tgtttttcaa gccttcgctg ccggatcttg aattgtttag ttcgaaaatt
     9781 aatatacacg acaaccaggc cggctaaaag tcctgcgcag gctgcataga accaccaacg
     9841 ctgataccag ggagtgctga taacaaaact gatttcttta taggaataat tatctctgcc
     9901 aaagccattc atttttnnnn nnnnnnatat tataattgcc cggtgccagg ttgttgaaac
     9961 tgaatacagc cccgttgtca gtatcaatag gccgccagga atcatcatct ccaagctggt
    10021 attccaggta aacattttct ttattgcacc atgctgaaaa tcccaatcgt atatgtatat
    10081 catgtttgcc gggcgaaagt ggtgtttggc caaataagga tgattcaacc cgtttattat
    10141 ctaccattac ttcatccgca aaaatctccc catccggcaa agcaggaata gcttcttttg
    10201 gatttaccca tagaagtccg tccattgtcg gaaaggagat agtgttattc ctgagggtaa
    10261 tggcacaagg aaggcaacct ccgttcattt ccgtcattac cattccgtca ttaagtccat
    10321 agtagtgata atatacccgg ggaagatcgt tttcatatgc atcgatcata tcagcgatgc
    10381 tggttttgaa aagcccatgg ttggtactga tccagcagaa cccctcctca tcttccacaa
    10441 aacaatgagt aaattgtaaa taacggtttt tatctaatgg cagcggttta accttcccgt
    10501 tcttgcttat ataaaggcca ttgnnnnnnn nnnnnnnnnn tccgaaaaac aagtactctt
    10561 tgtattgcca gatagtcctg acacaataat taccggacag gtaaattttt tctgttttgt
    10621 ttttggcaat atcatatcgt aacaacgcat tgcaattggc aatgtaaagt ataccgggtt
    10681 cggtttcttt aatgtcaaaa tgaatctgtg gtttctcggt tttggaatat gaaaagagca
    10741 ttttcaacga atctccttcg aggcgggaaa ttcccaattc attggctaca tataaaatac
    10801 cgttgacaga agtcattacc agctgtgaat agctttcacg gatcttatca aaccgtttaa
    10861 gcgttttcgt tttaaggtta tagcagttca gccgggaccc gccagacccc atgttctgtg
    10921 ctgtgaacca gaacagggaa tcacccatca ggtaggtgct tatactaaat ttttggatgc
    10981 cggttgaagt acgatattcc tgatcttttt ttcccaatac atgtccttca ttcgtcataa
    11041 cagttccatc gggcagttcc agttgtgaat aataggaagt tctttcatca acggcggtcc
    11101 gcttttttcg gagttgtgtt attttgtttt ttcggataat gataattcct tttgagtcag
    11161 ttccgagaaa taaagtaccc cgctgttcat catattgtac atatcggatc aatgcatcct
    11221 gcggcactgc cgtacaaata ttggtagcga tgagtttctc attttcatag gagatcttcc
    11281 aggcgttcgc accatcaatc actatgggat attccatgcc gttttcccag gctaaaaagc
    11341 ttttcgttaa taacggcttc tgcacattag cagtcctaaa ttctattttg gttttaccaa
    11401 gcgatttgtt aaaggggtcg atcagccata tattgttatt ctcgtcacag gtgaacactc
    11461 tgcccctgca cataaaggaa gatctgagtt gttttatcgg aaaaggcaat agaacaggtg
    11521 aagccactga tatcgaaaaa taaaatacct tatcatagcg gttgataatg catagcgtat
    11581 catttaccgg caatacacgg tcaaattgta aggtaaaggg tccgttgacg aactggtgtt
    11641 tctttttaaa aagggtttca gatacactaa gcgaaatagc attgttcttg ctatttcctg
    11701 agatacttat cttttcgtaa aaagaaagat tgtttttttg aacagaaaaa atattaccgg
    11761 tgttatctgc cgtgtatatc ctcccggtgt tgttctttac aagaaaaagc attcgttcat
    11821 tggtaatctg tttattgtca tcgttggtaa aagttttaaa tgtcatcccg ttgtacctta
    11881 ccaggccagc ctctgtagca acccaaagaa aaccggtttg cctgtcccac tgcattcctt
    11941 ttatgccgtt ggaaggaagg ccgtcctcgg taccataatg ggcaatacga taatcttctg
    12001 tttgcccaac agcagcaaaa taacaggtaa gaaggagtgt aataattgag caccgaagag
    12061 ccataactga ttgtattacc aagttacagc atcccgccta ataatttaca ttataaagtg
    12121 ttgccagctc cagcaactct tttatattgc cggcattcgt tttttcataa atgcggcttt
    12181 tgagggtgga aacagtattc attttcaggt taagtgtttc cgaaatttct ttggtgccaa
    12241 tacccttcag tacatagtgc agaatttcca gttcccttgg tgccagcgat ttgaaaggat
    12301 tatcatgatg gggcagtaca gttttacgga caggggcttc attctgtatg aattttttta
    12361 aaacctggat gatctcttct tcgccggcag atttggataa ataataatta ataccaaatt
    12421 gctttaccgc ctcaccataa atttctgccg gttgcataga gaagatcatg atagaaagat
    12481 ccgggtatac ccgtcgtata ttgggaatta cttccagggt actgccatcc gataagataa
    12541 tatcaagaat aagatgagtg tatctttttt tgacaagctc tttcatcagg ccgccacagc
    12601 tatcaacctc ctggatatcg gagtacccaa ggttcagttg caaataaagc ttaagccctt
    12661 ttctgatcat actatgatca tcggccaata atatctgtgg gtgcatttgg gttcaaaact
    12721 attttataat cgttaccgtt cccttgaaaa actgcttttt agcatcatta agcacaatag
    12781 tatatacaaa ggtatcacca gactgctgta tacccttata ctttccatcc cacgggttat
    12841 tgatataatt ttttgattca aatatttttt ccccccacct gttgtagaca gtaactacag
    12901 catccgggta aagggcaagc cccgggatat tccacttatc attgcgtcca tcattgttgg
    12961 gggtaaaggc agtgggtata tacaactggg aaacagggat aatggtaacg gcgtctttac
    13021 ctgtacaaaa tgttgtacga tctgttacct caagcgtata agttactggt gtaaaggccg
    13081 gggtggaaac cgggttcaat acggtaacag atgataaata atttccgggt gtccagagat
    13141 aactataact gcccgcagga gaaactgttg catccaaagt ggtggaagta tttacactta
    13201 tgtatttatc cggtccggca tttactgatg gagcagaatt gatagaaata ttctgtgtaa
    13261 aaggaggaga tccgcaacaa cttgtatcgg taatcacaag cgacacggtg tagttaccgc
    13321 tgttggtgaa aggcctgttg aacggaggta cctgcttgcc annnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaatg
    13501 tttacggtga tagtatccgg ctggcaaccg gtagtaaatg tggtagtgtg cctgaccggt
    13561 atattggtgc cagctgccgg aaaactatgt gtggtactgt tgctggttga tgaagtaact
    13621 gtttgcccgc cgccaaaatc ccaataccag gttgtggggt tggaagcgtt gggtgttatt
    13681 gatgaagtga atgttaatac actgtcttta caacggttgc ctgttatggt aaaagaaagt
    13741 tgcggaccag taccattaat gatgttgatt gtttttattg tatcaccaat gcagccatta
    13801 tcagcataca cccggtagcg gacatcatga ttgccggtag ttgcaaatct tttgcgggca
    13861 tcttttgtgt tttgtgtgct gccgtcatcn nnnnnnnnna aaattccaca ggtagctggt
    13921 ataattaaat aacccgttta cagtagaagt agtacctgta aaacgaacgg tatcactcag
    13981 gcatagttga ttggagatag taaaatcagc aggtgggccg ggttttacca gaacagtcaa
    14041 ttctacattt tcggtttggt tgcaattttc aattacggtg gatgaataag caattggtat
    14101 tttataggta cccggtgcat tgaaaataaa atcctgttgc agggtataaa catagtaggt
    14161 tctgccattg atcacttcgg tgcgaacaag aggcggattc gccaccaccg aatctgcatt
    14221 tgggctaatg cctggtacct ggctcagttt ccaggtaatg gaattggccg catgagctaa
    14281 tttggcatac aacctgacgg gtgttttggg gcaggtgaaa gtgtcaattt ttccgtttgt
    14341 attcagggtg ttttgtatct ggctatagct gttcaggttg ttgacaaaag tgccgacatt
    14401 gtaaccatag ctttcaaaaa aaccaagacc ataaatagta gcattgaaaa cggaatcgca
    14461 ggtgagccta tgttgtccgg cggcaccagt tattctggcg atagctacag aatatgaagg
    14521 attgttggga tgggtgataa tattaaccgg gtcaaaagga ttaccatcta tgcgtaaaga
    14581 aggtatgcct gttgattgta ccaacacatt cagatagtta aaatcaatga atgtttttct
    14641 gttggcataa aataatgcat ccttactacc ctgctctatt ggacttagat aaatcatctc
    14701 tggatcgccg tatgcaaatt gactattgtt ccagcagcgg ttgccacccg gcgtatattg
    14761 tgccaccatg atgggtttat cagcggttat ataatctcct ccgatactgt caatgatctc
    14821 atagtaaaaa ttattgatca atcctgtcat tggaacaccg tttcttctga ctactgtagt
    14881 aggatcatca actgcaatcc gataaaaatt tttaaatgga tcgtttatat cggtatttgt
    14941 attatttatc atgtggtagg taagataccg ggtgccccag gcttgtgtgg gaaacgcctg
    15001 ctgcatcatt acctctccac catcaaaata gcaaaagcgt atgccaccac taccggagaa
    15061 aagggcaaaa ggatgacagt taccgtcgtt gccgggtacc gataccactt tgcttccggt
    15121 cagatccttg cttgcctgcc aaagctggtt accgccacta actagtttgc ccattacatt
    15181 ataaacatct cccctgttaa ggtttactgt aaatgtttgt ccgggtagca ccccgctctg
    15241 tgtagtatcc gaaggggtta tttcaagccg ggtattatct tccgaggcaa caacatagaa
    15301 ccaggaatac caattggtgc cgttggcagt ggtattggaa taagagtagg gaactctgga
    15361 attcgattgt gactgagaat agttaagcga ataatatttg taaccaaagg cttccacagg
    15421 tatcagcata gttgcacccg atgtttgggt attatacata tgagcaaata caacaattgg
    15481 agtatcactt attatatgta tggccttatt gtttaaacct tctgcagtga tacgggcatc
    15541 atttgtccca gactttggta ttaatatcgt tgcatcaact gtattggccg gaatattcag
    15601 cgtctgactc cagccggtat taggtataga aacagttact gttgcggcag cttcagttgt
    15661 aaggtatagg ttcagttctt gtgaattggg tggcagggct ccgtctggaa taaagaacca
    15721 ggaattatat ccatagccca gccagaactc tttgcccttg tttgatatat tctgctgata
    15781 agcagtggta tgccaaaaaa gtaaaatgat aaatatggtt aaccccctga aatgcatagt
    15841 ttattaaagg tcaatattca gcagaataac aaaaatcacc agtagaattt attctacaat
    15901 ccaaaattgg ctaatggtct aaccttgctt tcctttttta tccaaaacgc aaaatttaag
    15961 caaaatgaat aaaagctgct ttgggaaatt gcctgcttca ttattggcaa ttctattcct
    16021 gttattcaca atggttgctg ccgggcaggg attttataac actaccaact ggcggttcag
    16081 caaccccaaa caattcggat ttgctgttat agacatggac ttttttgata ataacaacgg
    16141 tattgctgtt ggggccaatg gcggtattgc ctatacaaaa aatggcggca ccacctggag
    16201 ctatggtaat ttctccttca tcaaccagtc gggcctgcaa acgtcaacat catttcagga
    16261 tgttcacttt atatcagcaa caactgctta tgctgtaggc agcaatggtt gcatggcaaa
    16321 aactaccgat ggtggtgtta catggaatcg ggtgaacaac ccgctttttg ccaatgcaag
    16381 aaatgtaaat accgtttggt ttttgaatga aaataaaggg tatatcggcg ggcagcacaa
    16441 caacacaccc gatcttactc ccaaacttta tttcacactt aacggcggcg ctacctggga
    16501 ttcgatatcg gctccggtag gcgggaaaac aagagtgggc tatgtaaata acccggtggg
    16561 ttctttttta tgggacataa atgcaaaaga taaagaacta taccgcatca tattttggga
    16621 tgataacaca gggtatatct gtggaagcgg attaagcact tttgagccca tacctaatgt
    16681 aagcagcacc tctccttgta atccaacggg taccaataca tccaccggct cccatcatgc
    16741 aagcctgttg tggaagtttt caaatggcac attaaccgat tattcaatca gtaaagaaag
    16801 attaggatat aacggcatat atacaacact gccgggcgcc tgcaactatc ggtacgcctc
    16861 caactcaata catacacaaa cgtataaggc tatgcacatt attgatgcca ataatgtatt
    16921 gcttgtttct ttgaataaca atattgttgt tcgtgttaac actgccccgg gtgccatcac
    16981 acctaacatt aacgcaccgg gagtaaatga gataggaact tatcagttgc tcaatgcccc
    17041 aacgcctcct caaaataatt cttcagcact tggtgcacca attccggcca accctgcatt
    17101 tgggtttagc cagccaacga atattgttaa agcagccaac ggcaaactgt ttaccaatgt
    17161 taattcaccg gttctttttc ctgtaaacag gatgatgact tctgcggata acggagctac
    17221 ctggagagag gaaagatggt taccaacggg aagaaattac agtgagtttg gtggtcaggc
    17281 cattgatatt cttccttcag gaaaatttgt tgcagctgga caaaatggtg taatatctga
    17341 ttcgattccc ggcggtcaat ggaacagcac atatgtacag ggtacaactg gttcattcaa
    17401 taaaatggat gtagccgatt gcaataacat cattgcggcc ggtggcggaa ctattgcccg
    17461 caccagggat ggcggaaaaa catgggatca ggttgtaagg caggatttca ttaatctgaa
    17521 catcagcatc aactcagttg cctatgcaac gggtaatccc gccaaagcct attttgccac
    17581 cagcgttggt aatatttaca gaactacgga tatgaatgtt gccccgccgg cactgcccac
    17641 cctgtatatt ctttgtcggg tttccaaatg tgggatgttg ccacatcggg gaatgatagt
    17701 gtatgggtat gcggttacac tccaactcct tctacagcgc aggtttcaag agtatttcgt
    17761 tcaactaatg gtggaaatac atggtcacaa gcaacagcat ttccttccaa tccaagtgca
    17821 acaaattatt tactcaggca tattgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gttttccttg caggaaacgg tttccccagg
    18061 aaagcaattt tcagaacaac ggatggcgga aacacctggc aggacattac aggaaatgcg
    18121 cttgctattt tcccggttgg aaatttcaac tctgttgttt ttcatgatat taataacggt
    18181 tatgttggtt gtgcaggagg gttcctggtt accagcaatg gtggtgtaac ctggagaatt
    18241 gatcagacag catcacctac caatcacaca agtattgcat ttgcacctaa aaaagtacca
    18301 gcaggaacac cagcagctaa cagaaggctt ttctcggtag gtgtttttag taatcacatt
    18361 cttgaatatg gcgatactac caacctgaat gtttcatctt ctgagttgat ggtgtcatcc
    18421 tgtaataata cagccaacgg ttcaataaca gttattgctg ctggcggcat accaccctat
    18481 acctatagta ttgatggagg ggccttccaa tcaacaggaa cgtttaataa tgtaagtgca
    18541 ggaaatcata cgatcaccat cagggatttt gcctgcggcc ttattacaaa gacagtaata
    18601 gtgccggtac gtcctgctcc ttcagttagt gcagggcctg ataaaacaat cgtagaaggg
    18661 gatgaagtaa tgctgcaggg aagcagcagt ggtaaccccg ccactattgt atgggcacct
    18721 aataatacta ttttgtcagc aggaaataca ttgaccccga tagtaagacc gcttacttct
    18781 actacttata caatgacggt tacggatgcc aatggctgta tatctgctga taatgcacaa
    18841 gtgacagtac taccgtattg tttaaaggta atgaacgcct ttactccgaa tggagatgga
    18901 accaatgacc gctggatcgt aaccaacaat ggtggccttt gtgtaaccaa tgtatatgtt
    18961 acagttttca accggtacgg cggcgaggtt tatgtgaacc ctaactacca gaacaactgg
    19021 gatggcacat acaaaggaag tcctgttccg gatgctacgt attactatgt gatcacatat
    19081 acacttttca acggaagaaa agtaactgtg aaaggtgatg taacaatttt gagataatta
    19141 ttcaaacaat actattatga aaaagtatca attcattctt gtctgcctgc tggctgcagt
    19201 tgtgagcggt aaagcacagc agttacagac atcttctatg tatgatctgc agggagttta
    19261 tcacaatccc tctacggcgg gtacacaggg ttttaatttt gtcggggctt cctatcgtac
    19321 acagtggagt ggattcagcg gcagtccaaa aacggcaacg gtattcggat cgtttgaatt
    19381 gccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnccacttcc agaaccggta tacagctagc ttttgcaaag catattccgt tgaataatgg
    19501 aggcaagttt tcactaggta tcgaagcaag gggccttcag tttgctatag acagggccaa
    19561 attatctgaa acacttggcg ctgatcctgc cctgggcaca tcagaaaacc gctttaagtt
    19621 tgatgcaggt tttggaattt catataatgg taaaaaacta caactgggtg tttccgcatc
    19681 tcagctgatt cagtcaaagc ttgatttcta ctctggcaac ctgacaccaa ctgaagccgg
    19741 tcgcctgtac cgtcattatt attttcatgg cgcctacagg tggaatgtgg atggcgccac
    19801 tactattact cctaatgcat tggttattta tcttcccaat gcgccggcta cttaccagct
    19861 tggtgcaagg gtggagcata acgaagtttt ctggtgggga ttgggatata gggttgatca
    19921 gagttttatt ttatctgccg gtgtaaatgt gcacaagaag ttcacnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    20041 tattgcggtt caatatgcac aggtctaaat agcgttaata aaaaagcaga aggttaatgg
    20101 ttatggttag acggccccgt atttacgggg ctgtaccttt tttacgaatg caaaaaccta
    20161 attttacttt ttaaataaca acaacattgt caaaccttct tgtcaccgtc attcaaacca
    20221 atcttcactg ggaagataaa aaggcaaacc tgcaaatgct ggaagagaag attggtaata
    20281 tcaaggagcg aacagaagtg gtggtgcttc cagaaatgtt cagtaccggc tttagtatga
    20341 agccggagca actggcggaa acaatggaag aagaaactgt gcaatggatg aaaagaatgg
    20401 cggcagaaaa gaaaatcatt ctgaccggga gcgttataat aaaggagaaa gaaaattatt
    20461 acaacaggct gatatggatg ctgccaaacg ggcaatatgg cgtttatgac aagcggcacc
    20521 gctttgcttt tgccggggaa gacaaacatt atacagcggg gactaaaaga ttgattgctt
    20581 ccgtaaaggg ctggaagatt aacctgcagg tttgttatga cctgcggttc cctgtctggg
    20641 cccgccagca atcacagccg gatgggcccg aatatgatat attaatttat gtagctaact
    20701 ggcctgaaag aagaagccat gcctggaaaa cattattgca ggcaagggcc atcgaaaacc
    20761 aatgttatgt gataggagta aacagggttg gcaacgatgg caacgccata taccacagtg
    20821 gtgatagtat gattattgat ccgatgggag aagtgctgta tcataaaaaa gacgaagaag
    20881 atgttttcac catttcattc gataagaaac acctggaaac aatcagggaa aaacttccat
    20941 tcttaagaga tgcagatgga tttatgatct tgaatgaagc agaataacag gctatcgctc
    21001 agctttgtct atttttatca gctccatcac cttttctact ttaaagtctg catttcgttt
    21061 gatagcgtca atggtgggaa atgattcaat ttccggctga acacccctgc cgttcttagg
    21121 cgttgtttta tcaatgacca gccggaacaa tggcagcctg aaacggatac ctgttaccgg
    21181 taatgttaca tcaggaatca gccaggcgga atttccataa gccccgcccc ctgtttcttc
    21241 acccaccact gtgatattat cctgtttgac cagggcagaa gcaaacaatg tagtagctga
    21301 aaaggaatta ccacctgtta caatatacac tttgccatcg tagtgatggc gtttctttgg
    21361 tttgaaataa tgtctttcaa aataaccaaa gtgataatta ccatctttct tctttcgggt
    21421 gaaaaacaac ataaagagtt tgttccagaa atgatgttca acataccttt catacccact
    21481 gcctcttcgt atggcaaaca gtgaatcact gattttaaat ttttgtgcag caaggtaccg
    21541 ggtgatcagc gtagaatttg taacgctgcc accgccattt cctcttacat caatgatcaa
    21601 atgacctatt ttattttttt ccagtgcttc aaaagagtta tgaaagaatc ttcttagccc
    21661 aactccttta ccaaaagttg ccaggtccat catggctgta cggtttactg tatcaatacg
    21721 caataaccgg gcagcattta atcttaattc tcttcgctgt ttcttggtca gttgttgcgc
    21781 tggcctgaac gtacgggtac ctgttcttcc ggctgtatct gtggccggat cataaaccgg
    21841 aacaacagtt gttttaatag taccagtact gtctttgaac tcaatgtcat atttatcagc
    21901 ggggccaaaa agcaatgtat aaagtgaacc aaaatttccc ctgttgctta gtgattgata
    21961 tttgtgtgtg cggttatagc cgtcggtaga tatataatca aacaatgtat caactactgc
    22021 ttctgcagtc cggccgttta tcctggtaat tacagtgcct cgtttcaaaa tactgtcgcg
    22081 gcggttaaga ttggcattca ccaacatggc catatcccaa accttcatgc ttaatggaaa
    22141 catcttgcca agcctggcgg tatcactgta gcggctccag gctttggaag gtctcacagt
    22201 tgtatggcca caactgattt ttgctgtaac ataagtaagc actttgcgga atgctggttc
    22261 tgtcatggag tctttgagct gatgctggcc atattcaaag tagtaatcca tgctgtcttt
    22321 gggagtatac caatacaagc tggggtgatg cttttccagc acctggcggt aaactgaata
    22381 atcctccatc agttccgcag gagaatattt tttataggga gaaaatgagg atttgctcac
    22441 accgcatcct gccagtcccc ccgccgccag taataatatc agaagtatcc tcatgatcct
    22501 ttcaaatccc attaactatt gttctgtttc aggtgattcc atccatgtgc ggtgatggca
    22561 tgttttccgc ctcctttggt tatgatatga gctccctggt ctggttctgt catatagccc
    22621 accacactta tttgttcatt cagcaccaac ttttcataat ctgattgtgc tatggtaaac
    22681 agcagttcat aatcttctcc gccacttaac gcacaagcag tcgggtctat ttcaaattta
    22741 aaggctgctt tcttcatttc ctcagctata ggtatttttt cttcgtataa cacacatcca
    22801 agagcacttt ctttacatat atgcagtatt tcagaactta atccatcact gatatctatc
    22861 atagctgttg gttgaatttc ttcttttgca aaaaattcaa caatatcttt tcttgcttct
    22921 ggttttaata accgcccgat tacatatgat tcgccttcca aatccggttg cacacccgga
    22981 ctttccatat atatcttttt ttctctttcg agaaacaaca aacccacata ggcagcacca
    23041 aggtctccgc taacgcaaag caggtctcct ttttttgccg tacttctttt tacatatgta
    23101 tcaggagtca cttcccctat tgctgttacc gaaataacta aacccttttg agaagaagtg
    23161 gtgtcgccac cgaccaggtc cacaccatat ttattgcagg ctgcgtatac gccttcataa
    23221 aactcatcaa tagcctccac gctaaaacga ttactgatac caatactcat gatgacctga
    23281 gtgggaatag cattcatggc atagacatca ctcaggttga cgatcacact tttataaccc
    23341 aaatgtttta acggagtata caccaggtca aaatgaacac cttccagtaa caggtcagtg
    23401 gtaatgactg tttgcttacc aaaatgatca atcacggcag catcgtctcc cactcctaaa
    23461 acagacgaag cgttttgtaa ttcaatattc ttagtcagat ggtcgatcag cccaaattca
    23521 ccgagactgc ttatttcagt acgttccttg ctcataattc tttttgatta ttaatgacag
    23581 ccaccatttc atcatgtatc tttccgtttg tggccaatac tttatgctga taaaccgaaa
    23641 acttatttcc ctggtgatcg gtaacttttc ctccggcctc ttctacgatc aggtaacccg
    23701 ctgcgctgtc ccacggttct aatttatgtt cataaaagcc atcaaatctg ccgcaggcta
    23761 cccagcaaag atcaatagca gctgaaccca ggcggcgaac tggtacgcct ttgcgaacaa
    23821 accggtcaaa tatctcaagc gggccgttgg gcatattgat ataagtgtaa ggaaaaccgg
    23881 tgacaagaca ggccctgata gcctgtgttt cattactcac ttttatagat ttatcattca
    23941 gtgtggcgcc tttgcctttt tctgcaaaaa agaactcatt catgtgcgga ttatacactg
    24001 cacccattac catttcacca ttatgttcga taccgatcga aacacagtta agcgggatac
    24061 catgcgcaaa gttcaccgtg ccgtcaatcg gatcgatgat ccatttgtat tgtgagtcag
    24121 caatgatctc tcccgtttct tccgtgagaa agtggtgatc gggatagttt ttccggataa
    24181 catccaggat ggctttttct gaagcatggt ctgcttctgt caccaggtta ttgacgcctt
    24241 ctttattact aatgataaaa tcacggttaa aaaaacggag tatttcagca gcaccggccc
    24301 ttacagcttc tgtgagcgtt gttttaagca tgggcaaaga taatcctgat ttcagattgc
    24361 agtcccgata atatatcggg attagacttt agatttgacc catatttatg cagctttcag
    24421 ttatcatagt caattacaat gtcaggtatt tcctggagca gtgcctttac tccgttcaaa
    24481 aggcatgcag gggactgaag gcagaggtga ttgtagtaga caataactca gcggacaata
    24541 gtgttgaatt tctctcttcc gggtttccat ctgttcagtt ccttattaat aacgaaaacc
    24601 ttggctttgc cagggcttgc aaccagggat ggcagagggc aaaaggagag tatattcttt
    24661 tcctgaaccc tgataccata gtgccggaag atggttttga aaaatgtctg tctttttttc
    24721 agacaaaccc cgatgccggt gcactgggtg taaaaatgct ggacggcagc ggtaaatttt
    24781 taaaagaatc caaaagggct ttcccttcac cgcttacctc tttatataaa ttgtgtggcc
    24841 tggctaagtt atttccccgt tcccgcacat ttgcccgtta tcacctcggc cacctggatg
    24901 aaaacaaaaa caacgaagtg gatgtactgg ctggtgcttt catgatgatc cggagagaag
    24961 tgctggaaaa ggtaggcagc tttgatgaga cctttttcat gtacggggaa gacattgatc
    25021 ttagttaccg tatacagcag gccgggtata agaactatta ttttgccggc accagtatca
    25081 tacattttaa aggagagagt acccgtaaag gcagcatgaa ttatgtgcgg atgttttaca
    25141 gggctatgag tgtttttgta aaaaaacatt atggaggcag ccgggcagga gtattcaatt
    25201 tcctgattca aacggccatc tggttcaggg ccttgcttac ggcaatgggc acatttatcc
    25261 gtcgtgccgg gcttcccctg attgatgcag gcctcatcct gctttcattc tggctgatga
    25321 aaaatgtttg gaatgaatat gtaagaacgg atatacacta tgaaaacaaa ttgctgtgga
    25381 tcgcttttcc cgcatttacc attttttatc tcatatccgc ttattatgcc ggcctgtacg
    25441 accgatggta taaacgttct gaactggtca ggtctacttt gatagcaaca ttggttttat
    25501 tagcggccta tgcgttattg ccagagcaat accggttttc aagggccatc attttatttg
    25561 gcgccttgct ggcatttgta ttgatcagcc tccttcgatg gttgctggta aagacagaag
    25621 tattgaacag tgataaggaa aaagaagacc gctacactac tgtcattgca ggaaacgctg
    25681 atgaatacag gcaaacaatg gaactgctgc aagtggcggg tttgcaacaa agaatattgg
    25741 gcagggtgga tcctgccgga agcgatgcca attcacttgg ttccgttaat aaacttaccc
    25801 agctaacgtc aacagttccc tttcgggaaa tcattttttg cgaaggaaaa ttatcgtttg
    25861 ctgatattat taaaaatatg cagcagcttc cccgcggaac gatcgccaaa atacatgcag
    25921 agggcagcca gagtattgtg ggcagttgct cgagtaacac agcaggggaa accgtaacca
    25981 aagaaaacgg ttttaaactg gcagatcctt ataacaggag attgaagagg ctgattgata
    26041 cgagcctggc attactggct ctcatcacct ttcctgttca tctttttttg gttaaaaaac
    26101 cgggatcttt tttggccaat tgttttacag tattgttggc acagaaaaca tgggtagggt
    26161 atgcaatacc ggataaaagc ctgccgcagt taaagccatc agtgctggcc tccaatggca
    26221 tacctgccac aaccactcag caactcccaa aagaaagcct tcaaatgatg gactattggt
    26281 atgcacggga ctatgagccg gccacagacc ttaaaatcat ccggaaaatg tatcggaaac
    26341 tgggcgggga ataactttgg tgatctgcgt acagcaatac tccatttctt atattcgtaa
    26401 ctttagatac caaccaacca cagcgcagaa tggctaccat tatcgatata aaactaccca
    26461 gggccctggc ggcagcttta cgaattccgc ccaacgaccc gagaagacag cagataaggg
    26521 tattaaaaaa attgttgaag aaggcaaggt ttacagagtt tggacagcag taccgctttg
    26581 atgaagcatt attgagtaag catcccggaa gaaaattcca ggaactggtg ccggtccatg
    26641 attataataa gatctatgaa gagtggtgga agaaaacatt ggacggtgtt cctgatgttg
    26701 cctggcctgg aaagatcaaa tattatgcat taagctccgg cacttccgaa gcagccagca
    26761 agtacattcc ggtaacaaga gatatgctgc gcagcaatac cgtcaattat ttaaagcagc
    26821 ttttcagcct gatgcgctat gaggaagcca ataagaaagc aatgacaaaa ggctttctga
    26881 tgattggtgg cgctactgat cttaaaaaag gaaaagcagg ctggtatgcc ggtgacctca
    26941 gcggtatcct ggctaaaaaa agaccttttt ggtttcagag cttttacaaa cccggggggc
    27001 gcatcgcagc tatgtccgac tggaaccaga aactgcatga aatagtagaa catgccaaag
    27061 aatgggatat tggatatata gtaggtgtac cggcatggtg ccagatgtgc atggaaatgg
    27121 ttattgaaca ttataaagta aaaaccattc atgatatatg gcccaacttt agcttttttg
    27181 ttcatggcgg tgtagcattt gaaccttata agaaggggtt tgaaaaacta ctggccaaac
    27241 ccattgttta tatagagaat tatttatcca gtgaaggatt tatcggcttt aaaacaagag
    27301 aacatgcaaa aggcatgaag ctgatattgg ataacaatat tttcttcgaa ttcgttcctt
    27361 ttgatgacaa aaattttgac cacgatggaa acatcattgc aagtcccgag gcgaaaatga
    27421 tacatgaagt ggaggaggga aaagaatacg cattactaat gagcaccaat tccggtgcat
    27481 ggcgctacct cattggcgat acaataaagt ttgttgatct ggaacggtgt gaagtagtaa
    27541 tcaccggccg caccaaacat ttccttagcc tgactggcga acacctgagt gtggaaaaca
    27601 tgaacaaagc catcgaactg gtgagtgaag agctcaatgt tagcatacca gagtatacgg
    27661 tggttggatt tcctcatgaa aatatgtttg cccaccgctg gtatgtagct actaatgata
    27721 aagtagatgc agaacagcta tgtaaacgaa tagatgagca tctccggcag ctgaacgatg
    27781 attatgccac agaaagagac agcgccttaa aagaagtatt cctggaagtg ttgccagaag
    27841 aaaagtttct tcagttcatg gagcaaaaag gaaaactggg cagccagcat aaatttccaa
    27901 gagtaatgaa aggaaaaatg ctggaacagt ggaaccgatt tttacaaacg ggtcacatct
    27961 gatctggttc atacttttcg tactttgtac ttcttacttc aatgtaaatg ttagaagcgg
    28021 tcattaaagg acttggtttt ggtttgctgc tgagtatttc agtcggtccg gttttgtttt
    28081 ccatcatcaa acaaagtctg aataatggcc ataagggagg ctttgctttt gtggcaggcg
    28141 tttctgcaag tgatatttcc ctggtattgc tgagcaacat ttttacaaca ttgctggaaa
    28201 gcctgaaatt gtataaaacg gaaataggaa ttgccgggtg catattcctc gtgtccatgg
    28261 ggatattctt tctctttttc aaaaaggtaa aagtgaacaa ggagggagag caggttttta
    28321 aattcaggaa aagagattac gccaaaatgt ttttgtcagg ctacctgatg aataccctga
    28381 acccggctat ctttattttt tggataacaa cttcgacagc cataatcggc catacggtga
    28441 acgaacgaat tgtgattttt accacttgcc ttttattcat gttgttgaca gattgcctga
    28501 aagtgttgct ggccggaaag atccgccgga aactaacacc gcacaatatt catatcatta
    28561 accgggttaa cggaatcata ttaattgtat ttggcattgc actgatatgg ggacttattt
    28621 tttatggcaa aaaactctga caacaggaca aagaattttt tgaggggatt atggaaatgg
    28681 accaaacgga tcttcctttg gcttttcatt ttccagttat tttatatcat tctcttaaaa
    28741 tgggtgaacc cacccatcac cgtcactcaa ctggtaagct gggtaagtgg tcatggactg
    28801 aaacgagatt atgtaagaag cagctccata tcacccaacg ctaaactggc tgttattgca
    28861 gcagaggacc agttgtttcc cgatcacagc gggtttgact ggaagagtat taagaaagcg
    28921 atgaagtaca atgagaaaaa accaggccgt atccgcgggg caagtaccat tagtcagcag
    28981 gtagccaaaa atgtttttct gtggcagggt cggagctggc taagaaaagg gctggaagta
    29041 tatttcactt ttatgatcga actgatctgg ggaaagaaaa gaattctgga gatgtacctg
    29101 aacgtagcgg aaatgggaga aggggtcttc gggattgaaa tggcagcgaa taaatatttc
    29161 aataaaccgg caaaaaacct gaccaggcag gaagcagcga tgatagcagc ctgcctgccc
    29221 aacccgaaac gatatacggt taagccgttg tctgcttatg tttcttcccg gtcacaattt
    29281 gtgatgcggc aaatgaataa cctgcagacc gatcctgata ttcaaaagct gattggcctt
    29341 tctcctcctt aattagtcca caaccagctc aatcttttcg ctgttgtggg tagacggata
    29401 agtttgatga ttgatagcaa agcgaaggaa atacttgccg gggcttaaag caggattaat
    29461 cgttatcgaa aaattctttt gttcgactgc ttctttcaat actataccgg taaaaatgtc
    29521 cctttgccat ttgcctgctt tatatatacc gatgcgtatc gtgtcacttg ctgaggcgga
    29581 tccgttaata aacctcttgt aatgttcggg tgcgattatt gagcatgtaa gatttactgc
    29641 ttcattttct ttgatacgta tctttcttgt cgggggggta atattaattt ttgaaaaaga
    29701 gataaatgaa gagtcgtatt tgtagcccac aaagccaatc ggtgttttca tagaatcagg
    29761 aaaccgccaa agatcccaga tgtctaaaaa atacactggt tttcccagca aagaatcttc
    29821 tatcggccag aaattataat tgttccttct ctccttgtat aaattgagtg aataggtggt
    29881 ttgaccggaa taaaaccagt acttggatgc ccgctgatac gaattgctga aaacaacagg
    29941 caatcccttt gttttttccc gcatctcctg cggccatttt ttccaggcat gatagcgttg
    30001 ccggatagct ttaacgggaa gaatatctgc aatcatgacc actctgccgg aaagaaccaa
    30061 caaaagggta accggcaata aacggtacaa ccatttttgc cattgcagtt gttcattcag
    30121 aaactggtgc gccagtataa tgactgccac cagggcagga gaagtccagt taccttctac
    30181 ttttcccctg aatgaactaa gcagaaaaaa aacataaata ccaataaggg tgaaacataa
    30241 agccctttct gtttttgttt tcgttttata caaaaatgcg gcaggcaaca atataaaacc
    30301 agcaacaggg cctgcaatta ataattgtcc taaaacatat tcgcttgtaa aagaaaacct
    30361 gtaagcgttt acattgcttt caaacaaatg ataacgaaag ctcacccagt catgctggta
    30421 ttgccaccac aggtgcgggg tgaataaaag aagggaaaaa ataccggcaa catataattg
    30481 atactttgtg aaaagcttca ggttggagag cagtgtaaaa aaaacaatca gcactgcgtg
    30541 gtacttactg taaagcaaaa aagcgatgga caggcccagc aggaatgtgt tcagcacgga
    30601 ttgaacagcc gcaaactttt tataacacca aaagaagagt gcagtgaaaa agatcagggg
    30661 aatatccggc acagctacaa aaccagttag ctgaataaca gccatagaca atgcaatggc
    30721 ataaaacagg aatgggtttt tgttttctgt cagcctttca atgatcgcca gcgaaagaat
    30781 atttaaaagc agaggaaaaa aacgtacacc cagttcattg ggaaaaatgg cataaccagc
    30841 tttaaccagc agggctgtca taggcggatg gtcaaaatag ccccagtcca ggtggcggga
    30901 aaaaacccag taataggctt catcgtcctg tagctcggta agcctggcct gtagtaaacc
    30961 aagcagcaac cacaaaccat aaaaaaggag gcggtgatgt tttgccaata gttgtttcat
    31021 gccgcagcga aagtaaacac aaatctcatt tcagcagggt atctacggcg ctaacagctc
    31081 tttgaagccg ttgctcataa ttaccagaaa tgtcaaccca tggaacagcc tggtttatca
    31141 tcaggtcttt gtagatagca tataattttt tacgggtgtg cagatcagga tattctctca
    31201 gttcatcgtc cacccagggc aaatccgtat tacaaagcag gtagaggtca tattttcgtt
    31261 ctactatctg gtccagtata aagcggtggc atttattgaa aacaaactca caccacactt
    31321 tcattacata catgtcggtg tcaatgaaca ggggcaggtg gccgccattt tccaggtagg
    31381 tttgtgattg attttccagg gtaatagtgt attcgtcttc caatgccagt tggcccttgg
    31441 caatcgtcag caggtcctca aactcgtaat tcgttccatt ggtcagcagg tattcacggg
    31501 caaactccgg gcaccacgca gtttcgtagt gctgtgccag ttgctcacat aaagtgcttt
    31561 tgccggtgct ttcaggtccg atgatgacga ttttcttgag catgacccga aagatacttt
    31621 cttgaatgat tatttctacc ttttctgaag gaataaaatt ttttacccga tttaaagtaa
    31681 aaacctttca tggtcgtctt ccattaaaag aacggcaatg catgtggcaa tggcaggaga
    31741 aaagcaaaat atcatacagg cggtaaaaga ctacggcagg caattgtttg gattcatccg
    31801 taaccgggta agttctcaag aagacgcaga agatatttta caggatgtct ggtatcagtt
    31861 cagcgcccag accaaaagcg aggacattgt tagtatcagc ggctggttgt accgggtggc
    31921 ccgaaacagg atcagtgaca gttataagag aaaaaaggaa aacagcctgg aggatatgat
    31981 ggcagaagat gaggaaggta tatggcaggt gccggagttt atgactgcag aaagtataga
    32041 tcccgaaaca ttacagttgc agaaaatgtt cagggaagaa ctggtccagg cccttgatga
    32101 acttccggat aatcaacgca gggtttttat ctggaatgaa ctggaggaca tgacactgca
    32161 gcagattgcc caaagggaaa aagaaaatat taaaacgatc atttcccgca aacgatatgc
    32221 ggtccaatac ctgcgaaaac gacttgacta tttaaacaac gaattcttta acgaataaaa
    32281 aacaaaaaac agaagtatga aaagtgttat cagaagaaaa ccatatttaa tccccttgtt
    32341 tatgattctc ggatttgggg cagtggcctt gtttggctgg attgtaatgc tgctttggaa
    32401 tgctgcatta gtacccgtaa caggtgcagg tattataagt ttttggcagg cattgggctt
    32461 gctaatactc agccggatat tggtgggtgg ttttggtggt ggtaaaggca ggggaagccg
    32521 tatgggagca agatggtcac atatgacccc cgaggaaaag gaacggttca aagaatattg
    32581 caggagcaga tggggaaaag ccaatccgga agagccaccc tttactgaag tacagtaaag
    32641 aaaaaagcaa caggttagaa agcctgttgt tttttagctt tactactcca ttcaaacaac
    32701 ccccatactg ccataaataa cagaatcaga taataaacac tggtgaaaac atagtgctta
    32761 acaaagtata ggggcataga agcaatattc gtagcgaccc accaatacca gctctccact
    32821 tttttcttcg tcatcagcca catgccggta aaagcagtag cagatgcaaa agcatctgcc
    32881 cagggaatgg catctggcgc aaaatctttt ttcaggtaag taagcagtaa aaaaatagtt
    32941 atgtaaaagg cgacaaagaa tgccaattga tacaaccacc atttccggtc agaaaaactg
    33001 atatgaacaa caggatgctt ttccttatct tttttagtcc acaataccca accatagata
    33061 ctcatcactg tataataaaa attgacagag gcctcaccga tcaggtctcc tttaaaactc
    33121 agccatatgt aaatgatagt attgataagc cctaccgggt ataccagtat gttttctttt
    33181 cggctgaacc acacactggc aataccggca aaaacggcga tgtattccag taatgatgtt
    33241 ttttgtaaac cttctataaa ctgatgccag atttcctgta aatccaatgt tataatttcc
    33301 tgtaaacata ataaaaaagc aaaccctgat aggtttgctt ttgattgcag ttgtatgtcg
    33361 gcttattcta gtgattttga aggaaaatat tagctgttac ttttcgcccc tgctccaggc
    33421 ccaatgtaat gcttggcagg taatgaatac ctgcatagaa tcttgacatg ccggcttctt
    33481 caccaatggc atagaaagaa agatatgttc ttggcgacat attcaaataa tcatatgtat
    33541 gatcagtaaa tgtttgtggg ctaccaaaca attttgtcat tacatcagct gctgcagccg
    33601 aaaggctggc atgggcagaa gaatattcag gatgtgcagg tgttccgaca agtgtactcc
    33661 agttgctata tcccattact tccctgatga aagttatcgg gcgaacaagg ttgtactggt
    33721 atttggtttg aaaacaagtg attaatgcat cattcacagc agcgccggtc aatgcatatg
    33781 ccaatgctgt tttatccagt tttgcttttt ccttactcac tacctgttga agaatactta
    33841 accagtgacc cggtgatgaa acacctggta tatctctcca gtacattgcc atcgccttct
    33901 gatcatcggt taccacttgt gaagcatcgt atacctgttt taccatttga taaaacggag
    33961 aagatggatc agttgaataa gcgatcggtg cattcaactg tgtattcgtt gtactaccag
    34021 caactatagt gcgattatta ccccagtaag gagtagcagg cgctgtattt gccggaggcg
    34081 tcggcttcca gagccccggg cctgttggga ctgtataagg agcacttgca tttttataac
    34141 catctgtttc tgaccaggcg aatactgcag cggctacatc ttttccgaat tgagcagata
    34201 cattgatttc attatttgat cttgttaaaa ggaattgctg tgtcaaagcc gattccaatg
    34261 aatcgatcgc agctttatca gttgcatttg catttggaaa aaatgatctg ttgattgcag
    34321 ccatcgctgc atttacattc gctgggtaat agtagttttt tgatttaagt gtagcaggca
    34381 gacctgtcaa tccattccat tttgctgacc acttcttttc tgtactggta agaccgggca
    34441 ccagtgattc aaaagcggca atcccggtat aggcaaaatg tcttgcaaaa ccatgattgg
    34501 aaacacctgt tgcgttttta atcaggcgca actggagtgt catccattta tccaatactt
    34561 cagatgaata gctggataca tgattttttt ctgtatcaat atcttgactt tttttgcacg
    34621 ataaaagaag ggtaagtgac ataatagatg ccacgataaa taatacatga acaaaaaatt
    34681 tgttcagttt gcttgttgtt gtcattgatt taaggtttaa tagttaatga ttttaattct
    34741 ttttggtaaa acggtaaata gctgtgtagg gcacttcaac ggttgcgctt tcactcatgg
    34801 gtgcagtagc cggcgcttta cctcctgaag tggcaatacg ctgtatatac gccacatctt
    34861 taaaaaaacc tgtaggtatc tttccgctct ttggcatcag cagcagccag tcaacactat
    34921 ttatatcagg agacggagct gtcttagccg gggaaaaatg ctgtgcgaaa atggagtctg
    34981 ccggagacaa ggtccaatgc ggcccagcgg catgtgttcc gatctttttg ccggtaacat
    35041 catacaggtc tgctagcggg gcaacgaaca cccattcaaa tacaccggga cttcctgctt
    35101 ttgcttgcga cttatacttc tgtacgcctt ctgcgtagta agttgctact cttgaattac
    35161 ctgcaggtaa attggacgga agatcaatca ccccaggtat gaccaggttt tcactttgat
    35221 tgatcttatg cgctgtcgga ttagtgtcga ccttatcttt tttgcagctt gtaatgatcg
    35281 cagcaaaaaa gatcatcgtt aaaaaacttc tcattttaat tatgttttgt tttttcattg
    35341 taaaaggtta aatggtttat agtttttgat taaagttgtt tttatttttc accataaata
    35401 atgagtgaac ttgcatcgat ctttaaatac tgatcgggat acttgcggtt cacaacatcg
    35461 aatacctctc tttttatttt ttctttcagg aactcatctg tattgcttaa agctgccaca
    35521 accggtgcag ccacttcatt catcaccgtc caatacaggt ccgttgttcc gcaattcatc
    35581 tgggatttta cttctgttac tgaaatattc ttcaatcctg cctgtttgaa tatatccgcc
    35641 atcaatcctt ctttggcggc acgaaacata ccaggcgaac cgggcgggga tgcaggcaat
    35701 tccatatgct gattgattgt acccatcatg gcagttatcc aaaaattctt ttccggaaca
    35761 ttccataccg aggtagcaat tcttccgcct ggctttaata cccggtgcat ttcttttgct
    35821 gctaaaagca tatcggggaa aaacataaaa ccaaatcgac aactgatggc atcaaaactg
    35881 ttgtcaagaa atgggatatc agatacatcg catgtaagtg tttcaatatt atttattccc
    35941 ctcagtaatg cattctccct cgctatatct accataccct cggctaaatc ggtaatcacg
    36001 actttaccat tttttacaag attggcaatc gaaagacctg gctcaccggt accagatgcg
    36061 atatctaaca ccacgtcatc attttcggga ttaagctgat gaataatttc atcacccatt
    36121 ggtttcagaa aatccataaa aagttcatcc cattttttcc atcctggaga aaatttattc
    36181 caggatgccc gttgctgttc acggataagt tcaagttgtt tttccatgtt aaaaattttt
    36241 aaataattga gtgttgtaag cggatagttt tagtaaactt tgtttgaata ttgcgacaca
    36301 aacatatcat caaggcaaag ttttgctgaa tatgttcttc cgaagtgcct tcctattttt
    36361 cccatcggaa tgttgtttaa aaaaatattt tatacttttt attttccaaa gagcggcgca
    36421 gataatataa aaggcggcat ttgttttccc aaaggcggaa cggttcttta atgaacttgt
    36481 ggaagaaaat attgtacgat aagttggtaa ctttaataag aaggatgtac cgctgatgta
    36541 caaatactgt attatagtat cattcttttt ttcatttgca actataaaaa gtatagggca
    36601 gttgctgaat gaaaagagct ttacccgtta tgcgacgggt gatggcttgt cagataatac
    36661 tgttacaggc attgaacaag atgctgcagg ttatatctgg ctggccactt tttcgggact
    36721 aaaccgctat gatggctcca ggtttataaa atatcacagc accaacgaca gcctttctcc
    36781 tgcaacagaa aattttgtga gaataaaccg gttggataaa gacagaattg cctttctgcc
    36841 tatggggctt catgttgtaa ataccaaaac caatgaacgg agaaaccttt ttattcccta
    36901 ccataataaa cagtaccagt ataaatacaa tatggtggag agggcagctg gtgatgataa
    36961 aggaaacctc tttgtgctta cccgttccgg cttttatcat tatgataaga actaccggtt
    37021 actattccgc tttgactatt tttcagagaa ggacctgccc actgaacatt tttactttgg
    37081 taatgattta ctcgaacttg acgataagcg attactgata acggcccgac cgggtttata
    37141 tatttataat aaggagaaaa aagagtttct aaaactatcc ccaggtcagc tccctttact
    37201 ggatgagttt acacatgaca atacctatta caagttcttc caagtaaaac gggggaaact
    37261 gcttgttaca aaatctgaat cggacactat tttttatatt gatatcagtg taaacaaaaa
    37321 aattgtttcc cgatcgccat ttacattcag taatgatatt attcagtggc gctcaaagct
    37381 gatacctgtt aatgacagct tgttttatat tacaggtcaa acatcaggtt tttattcact
    37441 gaaactaaac ccttcttctg gttcagtgca attcatgcct gaaaagtatt ttgcggatta
    37501 tctatgcaat gatttgctta cagataaaga aaatcggtta tgggtagcga ccaataatgg
    37561 cttattccgc cagaatgata accggcacca ggtacagaca acctatatat ccgatgaact
    37621 aaaggcaaag tttcctaacc tgagcctcac ttccatatat gtcacttctg ataaaatata
    37681 tgcaggggca agagccggcg ccggtttgct tgtatttgat aaaaaaacat tccggtttga
    37741 aaatagctgg ttcaatggtt ctgatgtgaa aaacaatacg gtttttaaaa ttgtatcgcc
    37801 atataactca tcacttatta ttggttcatt gggccctata tcattgtaca acaatacaaa
    37861 tggatcaata aagacaatgc atccacccgg ctggaatccc aacgactggg ccggcgattt
    37921 actgaaagac agccgcggcg atatctgggt tagctcttcg aatatttacc ggtacagacc
    37981 agcagaaaaa aaatttatac tcattccctt tagcccgtct atgccttcgg tccccgtggc
    38041 tattgctgaa gacaggaatg gcaatatttg gatggcaggt cacggcataa tacggtacaa
    38101 tgtttcctac agccgttttg atgaaaagat tgattcattc ccttatataa aaatgcccga
    38161 taaacaggtg ggcccccttg ctattgacag ggataacaat atctggtttg gcagcgccaa
    38221 caacggattg atcgggtata atattgacaa aaaattatac aggcatttta ccaccggtaa
    38281 cggattgccg ggcaatgata tttctgcact aatcataata ggaaataaac tatggattgc
    38341 ctgttatgcc ggccttgcct gtcttgacct cacatcctta caaataaaaa gatttggaaa
    38401 agatgaaggg tttcctgaaa tgccgctgca aaacagggcc ggtttctttt acgatagttc
    38461 acaacagcag ttgtatatcg gtttctccac cgcattggtc aggttcaatc cttacgaaat
    38521 actgactcct caaaagcaac cttctgtttt tatagaaaac atggatatcg ggaacaaaaa
    38581 aatttatttt cccggcagga ttgtaagcgc aacatggaaa caaaatgatt taaagttaac
    38641 cattggcgtc atcaacttta atgacggacg cagccagggc tatgcttacc gcatttttaa
    38701 aaatagtaac tcaccctggc aacagctcgg caatcagcca tcctttagtg tttccaatct
    38761 ttcaccgggt aaacaccgga tacaggtaaa agtattttca ctaaataacc aatggcccga
    38821 acaggtaaaa gaaattatcg tagaagtatt gcccccattc tggcaaaaag attggttcag
    38881 gattatggca accttgttgt cgctggcatt gatattttta tttatacaat ggagaatcgc
    38941 tgtggcccgt aaaaaagaaa tggaaaaaac acatgtacag aagctgaagg ccgacgacta
    39001 taaaaacaaa tatgaactgg agcaaatcac aaattatttc tcatcatcac tggcagataa
    39061 gaaatcagct gatgaagtat tgtgggatgt agctgaaaac ctgataggaa gattgaacta
    39121 tctggattgt atgatttact tgtggaataa tgataaaaca aaaatgattc aaaaggcggc
    39181 ctacggttta aaagcaaagc cggagttgct ttctgaacag gtatttgatg taaaatccgg
    39241 acagggtgtg gtaggatatg ttatgcaaac caaggaaccg gtgctgataa aagatacaag
    39301 aacggatagc cgatacaggg tggatgaagt aaggcgctta agtgaaattt gcgtacccat
    39361 catacataat aatgagttgc tcggtattat agattctgaa catcccgaac tgaattatta
    39421 ttccgagagg gatttgaaaa tcttgactac catcgccaca cttattggca acaaattaaa
    39481 gcaacttgaa tcagaacaaa cactggaagt aaaagaacag gaactcagca acattaatga
    39541 acagctggcc gaagccaaac tttcagccct gcaggcacag atgaacccgc attttgtttt
    39601 taatgcgctg aatagcatca agcggatgat attagatgga gatgatgata aagcatcccg
    39661 ctacctaagc aaatttgccc atatgatacg aatgacattg aatcattcca aagaaacatt
    39721 tgtaacaatt gccgaaaaca ttgagtatat caaaacctat cttggaatgg agcaactgag
    39781 gtttaatgat tcatttaaat ggacaattac aattgctgat aacattgacg ccgacgaaac
    39841 aaggatagca tcgttaatga tacagccgtt ggttgaaaat gccatctggc acggactgtt
    39901 gcattcggaa aaagagaaaa tcctttctat ttcttttacc cgcaatgaag atactattac
    39961 ctgtattatt gaggataacg gtattggcat ccggaaatct caaaaagcaa aagaacagca
    40021 tcaaagtaat catcgttctg ttggtttgga taatctctac aaccggataa aaattctaaa
    40081 tgaaaaattc aatgctggtt acagtcttat tatcacagat tgtagtgaga ataatgagaa
    40141 ctgtacaggt acaagagtaa cgcttaaatt tattattaac gacaattaaa aatattttca
    40201 tgaaagcaat tttggttgac gatgagcccg atggagtaaa agccctgcag atgatgctgg
    40261 gacgacattg cccgtcagta gacattgcag ctgtttgtaa taatgctgac acagcgaaac
    40321 agcagataga ggcgataaag ccggatgttg tttttcttga tatacaaatg cccggtaaaa
    40381 gcggcataga aatgattggt gaactgcctg agaaaaaatt tgaaatcata tttgtaacag
    40441 cacataatga gtatatgctg caggcgctac agtacagtgc agctgattat ttgcttaagc
    40501 cagtagatga agacagatta gctgaagcag tacaaagagt agaaaaaaga ctattggaag
    40561 gaaaaaaaga agaattgact gaaacattgt tgtataatat tggtaaagca ggccagccat
    40621 ctgaaatgcg gttgtgttta ccaacattga aaggatttat tattttgaaa ttggatgata
    40681 ttatttattg cgaggcagaa agaagttact ctgtttttca tatcgaagga aggaaaccgg
    40741 taactgtttc aaagccgttg attgaatacg aaaacatgtt aacaaacaca tccttctttc
//
LOCUS       DAORLS010000003        36565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_589_length_36565_cov_2.210107, whole genome shotgun sequence.
ACCESSION   DAORLS010000003 DAORLS010000000
VERSION     DAORLS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 36565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36565
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_36565_cov_2.210107"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>424)
                     /locus_tag="PK977_00520"
     CDS             complement(<1..>424)
                     /locus_tag="PK977_00520"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF16608.1"
                     /translation="NKNPKNQWGLSYSRRIDRPAYQDLNPFEFKLDEYTFQKGNTLLR
                     PQYTNIITLTNTYKFKLNTSLSYSHVSDVFTQLVDTTDKSKSFMTKRNLATQDVFALN
                     ISYPFMYKAYTAFVNLSGNYSHYKADFGGGNRTIDLDVF"
     assembly_gap    425..459
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<460..>1684)
                     /locus_tag="PK977_00525"
     CDS             complement(<460..>1684)
                     /locus_tag="PK977_00525"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF16609.1"
                     /translation="LNVEGTINATGNDALELLRKSPGVLVDKDENLSLAGKNGVQVYI
                     DGKPSPLSGTDLTNYLKSLQSSQIEAIEIITNPSAKYDAAGNAGIINIRLKKNKSFGT
                     NGSVNAGYNIGIYGKYNGGLSLNHRNKSINVFGNYNYNHNLNENRFNLYRVQLDTIFD
                     GRSKMVNKNNSHGFKTGLDYFVSKKSTLGVLINGNIAETNMANDGRTPITYKPTGVTD
                     RLLDVNNRNQMDRTNLNFNLNYRYADTAGRELNLDADYGLFRLKSNQLQPNTYLNASG
                     TTILNQFIYRFVAPTDIDIYTFKGDYEQYYKKGRLGVGFKTAFINTNNNFGRYNVTQL
                     NPEVKQLDIPRSNHFDYTENVNALYVNYNKQLKGVMFQVGLRMENTNSRGDSYGLNGD
                     GSINKGSKQTFERKYT"
     assembly_gap    1685..1704
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1705..2073)
                     /locus_tag="PK977_00530"
     CDS             complement(<1705..2073)
                     /locus_tag="PK977_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF16610.1"
                     /translation="MRQLIVVLGTFILYSFSANSQKLSGIVIDEQGKGLDKTTISLLR
                     TKDSSVIKFSTTGNDGKFSIEATQGRYLISASHIGYTTYYSSQFELNGELLLDPVTLK
                     KIAKEMQGVTITAQKPIIEVK"
     gene            2263..2640
                     /gene="gcvH"
                     /locus_tag="PK977_00535"
     CDS             2263..2640
                     /gene="gcvH"
                     /locus_tag="PK977_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRF16611.1"
                     /translation="MNFPANLRYTKDHEWISLDGNIATIGITDYAQRELGDIVYVEVD
                     TIGKSLEAGAVFGTVEAVKTVSDLFLPVSGTVTELNPDLAGSPESVNNDPYGAGWMVK
                     MTVNNPADVEALLDAAAYEAMVG"
     gene            2713..3252
                     /locus_tag="PK977_00540"
     CDS             2713..3252
                     /locus_tag="PK977_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF16612.1"
                     /translation="MNLNTTYSEQELVDLLQHRNEKAFTYLYDNYSGALLGIVNAIVT
                     DKETANDVLQNVFINIWRRIESYDPSKGRLFTWMLNIARNAAIDEVRSKGYNNSQKNV
                     PFPESNEIAGAVTGTDITDVGLKKILGNLKGELRVLVDMSYFQGFTHEEISKALNLPL
                     GTVKTRIRTALIQLRTMIQ"
     gene            3257..4021
                     /locus_tag="PK977_00545"
     CDS             3257..4021
                     /locus_tag="PK977_00545"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRF16613.1"
                     /translation="MNVQEYISSGIVESYVLGLASEEERKEFEQYCQQYPEVMKARTD
                     FELVLEKQAMQNIIVPDPSLKSRIWNQIGVTGKVLPMVEPVPVRKMSWIKYVAAASVI
                     LLIVSLYWNISLFNKNQELKNNYDLASEKIKEMEHDKQVLQQNPHVKMASLKGMEASP
                     QSFATVYWDTASHDVYLLANNLPVPPTDKQYQLWALLDGQPIDLGMLDYYVKQKKLLV
                     RMKNAQKAQAFAITLEKKDRSDVSKPQGDMYVMGNL"
     gene            4192..4548
                     /locus_tag="PK977_00550"
     CDS             4192..4548
                     /locus_tag="PK977_00550"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRF16614.1"
                     /translation="MIKPSLLPAAGWLILCTILLVLPGSAFPKEDWLSKIWFDKWVHI
                     GLFSTMVVLWCWGGQKKFKKKYILVTTAALVYGIAMEFIQQYFVVNRSFDTGDIVADA
                     IGCLIGYGFSWKYIKK"
     gene            complement(4983..>5626)
                     /locus_tag="PK977_00555"
     CDS             complement(4983..>5626)
                     /locus_tag="PK977_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16615.1"
                     /translation="NHCIMLDQIVNLVKQFGQETVVNNPEIPNEYNQDVLADATSTIT
                     NGFQNVMAGGGFENILDLFKGGANNTSGSGIGNLVKNPIVTMMIGYFISKLVNKYRMS
                     PASASNVANNLIPNALSGLINQTADPENPNATLDNLVGSLTGGNAISSDQENNSGFSL
                     QNLLNQFTGGGTGSGGGLDLQDIISNLTQKAQSGFQNRQQSGGLMDMIKSFLN"
     assembly_gap    5627..5638
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5707..6771)
                     /gene="prfA"
                     /locus_tag="PK977_00560"
     CDS             complement(5707..6771)
                     /gene="prfA"
                     /locus_tag="PK977_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRF16616.1"
                     /translation="MLDRLEAIKARFDQLGIALTNPEIVGNNKKFAETSKEYRSLEKI
                     VVAYNDYKKILDDIEFYKEALEGGDEEMRELAKLEAPALEEKKEKQEAFIRQLLIPKD
                     PQDEKNAILEIRAGTGGDEASLFAGDLLRMYMRYCERRGWKTAILSENEGTVGGYKEV
                     QLEVTGDDVYGTLKFESGVHRVQRVPDTEASGRVHTSAATVAVMPEAEEVDFELNPAD
                     VKMETARSGGAGGQNVNKVETKVMLTHIPTGTVVVCQTERSQLGNREKAMQMLRTKLY
                     EEQVRKQEDEIARHRKSLVSTGDRSAKIRTYNFPQGRVTDHRIGLTLYNLDAVLNGDI
                     QELIEALQFAENAEKLTRQN"
     gene            6837..8012
                     /locus_tag="PK977_00565"
     CDS             6837..8012
                     /locus_tag="PK977_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF16617.1"
                     /translation="MNYRKFKADRIFDGYQFHQGKVLIIDEDGIFDNIVSEEEAGENV
                     QQFQGILAPGLINCHCHLELSHLKDVIPPHTGLIEFLCSVVTKRDFSPEVIQEAITAA
                     EKEMYDNGIVAVGDIGNTADTVDVKRKSQIRWQNFVEVLSFTDEKAEENIEHYRKVAA
                     TFNTVHGSWIMDNRTSLVPHAPYSISPKTFKLINQLTAGQIISFHNQEHPAEDELYKT
                     GGGEYLKLFKIFGLDQSPFPVTGKSSIRSVLPHFNNGQTIFLIHNTFMPEEDVTWANE
                     YATTNGLKLVYCLCINANLYIENKVPPIDILMKHNCQIVLGTDSYSSNWQLSIAKEIE
                     AIRKHFPHIPEETILQWATINGAKALQWEKKLGSFEKGKKPGISLINMGYSISTKLS"
     gene            complement(8004..8750)
                     /gene="recO"
                     /locus_tag="PK977_00570"
     CDS             complement(8004..8750)
                     /gene="recO"
                     /locus_tag="PK977_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRF16618.1"
                     /translation="MPDKLHKTKGIVLRTVKYGETSVIVTLFTELFGIQSYLVNGVRA
                     SSKKGTGKANMFQPAAILDLVVYHNELKQLNRIKEFKWSILYRHVLTDVPKNAVALFM
                     VELLTKCLKQPESNPDLFHFVEDCFLYLDNSSGTITANLPLFFALHLPGHFGFRIADN
                     YSERYHFLDLQEGGFTAIQPTHPHFLEEKQSAVSSQLLKVMQPEELDDIKLNHEFRRQ
                     LLHRYETYYALHIQDFGTMKSLPVLREVLG"
     gene            complement(8750..>9623)
                     /locus_tag="PK977_00575"
     CDS             complement(8750..>9623)
                     /locus_tag="PK977_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF16619.1"
                     /translation="FSLPFNVFENALSQIVIDDNNYKWIVSPKGNGLICYDHGASVDD
                     TNGDKWRRYGTGTGNGNLPSNDVVCVTKDKNGYIWIGTNDGVAVLQCPELAFSSPACD
                     AVWPIVPNGNFAGYLFKGQEVRSIAADGADRKWMATRNGVFLVSASGEKVIYQFTETN
                     SPLLSSDVKKIAIDGRTGEVFFATSKGICSFRSTATEGGEANENVLVFPNPVPPGFSG
                     TIAIRGLVNNAIVKITELDGRLVYQTRALGGQAVWDGRNYKGQRISSGAYLVLVSDDG
                     RKERTATKIFFISK"
     assembly_gap    9624..9633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9634..11057)
                     /locus_tag="PK977_00580"
     CDS             complement(<9634..11057)
                     /locus_tag="PK977_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16620.1"
                     /translation="MRKIVFIISLLITINVSAQDGIPPVGLWREHLPYNSAIDVTAGN
                     GKLFCATPYSLFSVSLTDNLIERYSRVTGLSETGVSAIRFDEAGEKLFIAYSNSNIDI
                     IYRNDIYNIPDIKRDNIIGDKNVYNIYSFSGNFFLSTGLGVIVIDGNRYEVKDSWFIG
                     AGGNQVKVNGFTSDATFYYAATEEGLKRITINSPAPGNHTNWQLISGTGGLSAGPCKN
                     VFTIQNRVIVQKNDSLFMQNGSNWSLLYTDGWPWVSSSVAENKILLCQRQISGVSRVV
                     ILNPDGTINRLLANTGAVSFPRKAILVNSQPWVADQFACLSRFEVSSSAYEQYMLNSP
                     QGIASGEMLVYQNVFYATAGSVNDAWNYLYNGDGVFSFKEGSWANTNRYRFSQLDSLL
                     DFICIAVDKRDETVWAGSYGGGLLHIKPGPSFEIFKQNNLSAVVGDPLSYRVSGLAFD
                     NDNNLWISNYGASQPLRVRKADGS"
     gene            11139..>12083
                     /locus_tag="PK977_00585"
     CDS             11139..>12083
                     /locus_tag="PK977_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRF16621.1"
                     /translation="MWQSLGRFILRYRLPLLLILATITAFMGYHASKVQLSYDFARAI
                     PINNPKYKVYQEFKKKFGEDGNLLVLGIQTEKLFEEKLFNSYADLQRKLKTVKGVDDI
                     ISVPTAVNLVKLPETEKLKAESIFPDRKLSQAEIDSAAATFLNLPFYRQLLYNPNTNA
                     WLMGIRINKDLMASKERLSIVHTIRQIADDFGKANNITVYKSGLPQIRTELTERITKE
                     MRLFIFASLILSAVILLLFFRSFSAMWLSLIVVFIGVIWSFATIQLFGYKISVLNALI
                     PPLIVVIGIPNCIYFLNKFHTSYNETGDKKASLVMMVDR"
     assembly_gap    12084..12102
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12838..13467
                     /locus_tag="PK977_00590"
     CDS             12838..13467
                     /locus_tag="PK977_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRF16622.1"
                     /translation="MADVGSKRLPLILDSIENRVNQLFDKEKYEVKMTGTSVTFLEGS
                     GYIINGLKDSIIWAFVLIAFCMLYLFRSARILLISLIPNIIPLLITAGIMGWANIPLK
                     PSTVLVFSVALGIAIDITIRFLVNYKQELPLNNFDMKQTVIETIHSTGISIIYTSLVL
                     IAGFVIFCFSGFDGTKALGWLTSLTLVTATFTNLVLLPAILISFIKMRK"
     gene            13682..16801
                     /locus_tag="PK977_00595"
     CDS             13682..16801
                     /locus_tag="PK977_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF16623.1"
                     /translation="MRKLFLLLFGVVFFAASALAQRTITGKVTDEKGAAIANASVIVK
                     GTTTGTTTKADGTFSLPIPANARVLVVSSVDMLTQELNISSQTSFEVVLKGEDKVMQE
                     VVVVGYGTQQKKAFTGSASKVDAKEFANLITPSIDKQLAGRATGVQVTNQGGLVNTPA
                     RIFIRGINSINNRTQPLFVVDGVPIIDGNLAATTNSNALGDINPADIENIEVLKDGSA
                     TAIFGSRAAAGVVLITTKKGIKGRSKVTYDMSLGFSNVLKRFDLLNAEEFVVISNEKF
                     ANAGVAPRAGVNPGGVNTDWLSESLIDNALVQNHNLSFQGGTAKTTFFFSANYSKQQG
                     VVKTNQNIAYRLRMNLEHEMNSFIKFGNNLTVTRQVDADQNNGANALSGSIASALRLL
                     PNVAAYNPLHPTGYNINFPNANSIPLGPNSASVDDNFTNVAFTLGTNKFTSDKYRIIN
                     NAFMELSFTKRLKFRSVGSADFFNDYSFQSLDPRHGDGFGSNGVAFNQNQNQLRYVWQ
                     NYFNYNLTGKKHSLYLTAGHEVQKQTYKTLSAQGTSIADFYFLKENLISGSASNQFVG
                     GGYAKEGFESLFGRINYDFKNKYFFQASIRRDGQSSLAKDKRYGNFPGYSFGWRVSEE
                     GAWKDNRFLSKWFPEFKLKYSYAKVGNTLTGFRYLPATFGPTLYGGINAIGLNFIGNP
                     DLLWETSTKFDFGAEIGMLNGRINLTFDWFKNDVDNLVLNVPTPASAGIPGNSISQNI
                     GQLVNKGLEFSIDIAVFKKKDFNWDVNFNYSNVKNKITKLYDINGVPVPYIQNGAYNL
                     IQVGQPINFIWGYKSAGVNTANGNPMYYKADGRLVQHNIANNTFYFISGPNDGTINPA
                     NATTMTIGDRQNLGQGVPVYFGAFTNNFRYKQFELEVMFRYSGGNEIMNVTRQEALMS
                     NSFHNNGREILQRWQKPGDVTNVPRLRWGQSNNINQNGLAISRFVESGDYIRLQNVVL
                     SYTFDKNLISRVFKGYVENIKFFLQGQNLAVWTDYTGADPDNISAGGVDAAVAPQIRT
                     ISFGLSVGF"
     gene            16829..18427
                     /locus_tag="PK977_00600"
     CDS             16829..18427
                     /locus_tag="PK977_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF16624.1"
                     /translation="MKQIKKYLALLPLFALGLTGCKKALDLVPYNSVAIEEAFSTPAK
                     CLASLYGVYDAAQSGAYIDGSIRGYPFGAANIEQGDCRGEDVINIAAFFQITYQATYN
                     ANSANCVGHWNSLYALINRANVSIDGFRQAGSSGVLSTAVAAQYEAECRFLRALSHHE
                     LMVMWARPYRENPNGIGVPYRDFAVKLPADVELVRTLPRSIADTVYKRMLRDLDYAEI
                     NLPVQFSGNDRSVRASRAAAIALKMRIKLHMGDWAGVITEGNKLIPAVINPLNPSSVV
                     SPIGPWSLTPAPDGPFANNISSESIFSVRNNNIDNTGVNGALAAMLGAANLGARGLVA
                     VSPIIWNNPGWLCDDLRRSLLYVSGINANNGTSIFTTKYRDYVNRGDFAPQIRYAEVL
                     LMQAEAEQRNSGSVSTRALDLLNTVRNRSLATPAAQQYVVGNFATPTDLIRAILLERR
                     IEFLLEGKRWGDISRLSQDAAFTTNGIPAKAINGASGAAIYNCGGAYTPGQAGIAYSD
                     FRFLWPIPAEEIIQNPVVTQNPGY"
     gene            complement(18544..19032)
                     /locus_tag="PK977_00605"
     CDS             complement(18544..19032)
                     /locus_tag="PK977_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16625.1"
                     /translation="MTAFLQLPLSVYVKLLIAFAVVIIFCRWLVKRMIKAGKSKAHKM
                     NAKRLIYAGFAYAAFVWLVFTMITFEKQTKFKRNKWIAERNERYKMADDLIQSGIITG
                     KDSNEVKALIGEPDLRNVTSQIWNYNMGIGGGGFSCTMHHLVVEFIADSVSNIWHQMI
                     AD"
     gene            complement(19351..>19576)
                     /locus_tag="PK977_00610"
     CDS             complement(19351..>19576)
                     /locus_tag="PK977_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16626.1"
                     /translation="LADFTTYLFPGAIGIFTFFDAGKVWVKNDPGNTKLAYGYGGGIW
                     FAPLSKLVLSATYAMSNEDKLPIITLGWRF"
     assembly_gap    19577..19605
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19606..>19888)
                     /locus_tag="PK977_00615"
     CDS             complement(<19606..>19888)
                     /locus_tag="PK977_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF16627.1"
                     /translation="DNRALPTKGIYWETNIRHLAGLKDNPYAVTQMNSDFSFYVPLSK
                     NNNRLVFANRFGGGTNVGSKGFEFYQAQYLGSEDNLRGFRRYRFAGKSKL"
     assembly_gap    19889..19943
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19944..>20264)
                     /locus_tag="PK977_00620"
     CDS             complement(<19944..>20264)
                     /locus_tag="PK977_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16628.1"
                     /translation="NEFIGVLGTKTDIVTDIDVKAPNNTTNFFGYGMNSVYDKSQTGK
                     FRYYRARYNLADASLLVRERFSPKFTVSFGPTFQRYELDSTDDKNSQRFITDVPANGL
                     DINT"
     assembly_gap    20265..20304
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20305..>21492)
                     /locus_tag="PK977_00625"
     CDS             complement(<20305..>21492)
                     /locus_tag="PK977_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16629.1"
                     /translation="VYIPDDPALGEFRQEFGNMLATFEDRLPEGVAKGYDTDEVAEKL
                     EKDNDNEVDQKALLVARILDMYVMDLDRHEDQWQWGAKENSKGGKTYFPIPRDRDQAF
                     YTNQGTLPRFASKKSFVPQLEGFKAQAKSIRHFNFAARNLDRFFLNGLSEQDWKTTVD
                     WFITKMTDEVIDKAIAQQPAEIRNINGPKIAETLKQRRNYLAAEVMDYYRFLAEIVSI
                     TGSDKKEFFDVTRNANGDILLQVYKIDKEGNQSSKMYERTFKAGETREIRLYGFDGTD
                     KFVVKGDNDRIKVRMIGGGDEDYFENTNRTRGSLFVYDKAESKNTVKGRVKNRMSNDS
                     TVNTFQRIYYKYDKAGPGVAVGFNPDDGLFLGLSYKFVNHGFRKEPYKSSHTIAASHA
                     LST"
     assembly_gap    21493..21510
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21511..21944)
                     /locus_tag="PK977_00630"
     CDS             complement(<21511..21944)
                     /locus_tag="PK977_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16630.1"
                     /translation="MRLQRLLVIAFLSTTTIFADAQQGKDSATAAGGKFTTNRARMRW
                     MGANYRTEWNTPVKVPVQSLTGLTPVKRGGGKQTKSLRMQDASGKQYTIRSIQKFITS
                     KTLPGGLNSEAAADLVADGVSASYPYASLSMQPLADAVGVPYG"
     gene            22264..>25644
                     /locus_tag="PK977_00635"
     CDS             22264..>25644
                     /locus_tag="PK977_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF16631.1"
                     /translation="MRKKVLLIGFLLVQVLVNAQKDSITQRIILIGDAGQLTNGKHPV
                     VDAVKKNIPLDNKTTVIYLGDNLYKSGLPDVEYKNYDIAKAVLDSQISIADGTKAKVY
                     MIPGNHDWENGKRGGYNAIIRQQLYVDFIKKKEDNVKYYPEDGCPGPVKVDLGKDVTL
                     ILFDSQWWIHPHEKPEIESDCNCKTKDELVTQIGDIAANNSKKLIILACHHPFKSNGA
                     HGGYFTLKQHIFPFTDLKRSLLIPLPVIGSAYPIARSVFGSPQDISHPAYTNMINRVT
                     AALKANAPNVIFTSGHDHNLQLIKEDGYNYVVSGGGCKENRTSKNTNSLFNTTYNGFS
                     VLEVTSNKDVNIKFYTVTDSVRLAYTSHLLNFSKLPEEVVLQAEHKDDPAAIRLDTIS
                     KAASVQYQPVSGLKQYFMGQNYRREWSAPVNMKVLHFDTEKGGLKIVSLGGGTQTRSL
                     RLADKSGKEWVLRTVKKYSNQAFAENVMGSSRDQFKPEISTAAHPYGSLIVPDLANAL
                     NLKVAKPELFYVPKDSIALGLYTKLFANNVCILEPRNFTEDGSETKTTAKIFFKNMLE
                     DNDHRADQLAVLKARLLDFIIGDFDRHFDQWRWATIDSADMKGKIYYPVPRDRDQAFF
                     NPTGKIFRLIGSREMPWLKGFKNEIKKVNWLGYTARDFDRIFLNNLTDKQWKTAADEV
                     VNNLSDSVIRNAVKQLPPEIFAISGEATIQKMISRRKQLEKEALDYYDFLSKEVNVVG
                     SNQKEYFKVSSHPEGLQVRVYEKSRKNDTSFIMYSRVFDPAVTKEIRLFGLNDEDVFE
                     IEENVSSPIRIRIIGGRGNDTFNIKGNVRNIIYDLNSDLNFIKSSNRTRNRFSDDPPA
                     NERSILGFSYNTLQFPQLMMGYNYDDGFMIGAGISRRTYGFLNLPYATDQQFSAIYAV
                     ERKALQLRYRGEFNQIGKKTDLLIRAGLNTPNITNFTGFGNNTNVPDGKNFDFYRIRY
                     RTVEAELLLRRRPFESLHLMAGPYFYQYSNNYADNNKTILAEPRQVGLDSADIFGKKS
                     YAGLKLAMQFDNRNNLLFPTRGLLWTNEIIATAGLGDKADNFTRFSSDMTIYVSQKDP
                     ARAVLVLGFGGSKVFSDDYEFFQAANIGNNNINIHGFRK"
     assembly_gap    25645..25659
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25660..25945
                     /locus_tag="PK977_00640"
     CDS             <25660..25945
                     /locus_tag="PK977_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16632.1"
                     /translation="RFLGKSSIYASAEARIKLFNINGYLLPGQFGITGFYDAGRVWYA
                     GESSKRWHGAYGGGLYFIPFNRFIISAAAGFSENERMLNFSLGTKMNISF"
     gene            25962..26585
                     /locus_tag="PK977_00645"
     CDS             25962..26585
                     /locus_tag="PK977_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF16633.1"
                     /translation="MTKRLIAGTALLTLIFVIWLVFIANNTRPDEVVFDLVAPYTNGE
                     RTDWMKKISFLGNHQFLIPANLLLIAFFISVKKHKDALLVAIVALSSLGLMSLLKNLV
                     QRYRPPAPLVDGITNFSFPSGHALMSVAFYGVLAFYAVRMINNPVIKITILLLLFAVT
                     LLIGFSRIYLRVHYTTDVIAGFSIGICWMLFCFWLLNKAAKPGNQGL"
     gene            complement(26573..27439)
                     /locus_tag="PK977_00650"
     CDS             complement(26573..27439)
                     /locus_tag="PK977_00650"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HRF16634.1"
                     /translation="MAFLKFRIKRFTYLLMAAALLSGCKPKKRVIKSPPHYNFAEVQS
                     VKLDLKLKEISGLAWDADRDEFLAHYDESGHLFVLDKATNVITADYKFGGKGDYEDVA
                     IYNGVPYVLRSDGMITKIIRDSAGVRGVEAGKLNITGTNDFETMYADTARKALIIVCK
                     NCGMDDKSKVSAFAFYPDSIGFVSNPVFQIDAAKIKQIATEAGHKKTSKFQPSAAGIH
                     PILNKLFIISSASSQLVICDLNGNPESVSELGEKLFPQPEGLTFKRNGDMYISNEGVT
                     SRSTILRFVYKP"
     gene            27483..28751
                     /locus_tag="PK977_00655"
     CDS             27483..28751
                     /locus_tag="PK977_00655"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HRF16635.1"
                     /translation="MGDNHLSILQEAQALVTDLLLNKLSKNIKFHTLQHTQEVVAACE
                     KMADHYQLGADDRFALMLAAWFHDTGYTSGDAKGHENVSIEIADEFMRARQLDDATIQ
                     KVKGCIGATRMPQSPATAIEKIICDADLFHLGTDSFDEKNRLLRQELIDFASNDVSKK
                     DWRKINIRFLERHEYFTDYAKQKLQPVKDKHLAELIGKEEKNQPTMKPEKKEKDKKDK
                     VKDPAAEAAAAEAKLKAQKEKEADRSIATVFRIMAQSHSNLSGMADSKANILISVNSI
                     ILSIMISTLFEKLQTDPNLQIPLGVITIVCVASIVFGILATRPNVTHGTFTRDDIASK
                     KTNLLFFGNYHNMNLEDYNWGMTEMLSDKNYMNASMIKDQYFLGVVLAKKYRYLRIAY
                     NIFMYGLILAMLSFVVAFLLPGSTEVYTAP"
     gene            28789..29454
                     /locus_tag="PK977_00660"
     CDS             28789..29454
                     /locus_tag="PK977_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16636.1"
                     /translation="MKKTLIGFAAIILFACNNSKKDPAPETNNQATDAPPTTTTTAPA
                     PAAASTNSVITIEGKELNLNGSILVDKDKKKLQAGAPYRGMITSSSGPNDEGYILRFV
                     FDTKPGVYPVTGISFGRGKDDNAQQFGGLLGGEEKIYDSKVTLTECKDLGDNGAGGHK
                     WSISGTVENVTIPAMGIMLLDKEKNHPKEIKIDKLSFTGLTFDDNAEEMLEKAMEMLK
                     KKN"
     gene            complement(29527..31383)
                     /locus_tag="PK977_00665"
     CDS             complement(29527..31383)
                     /locus_tag="PK977_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRF16637.1"
                     /translation="MARKQEVLSDVVIKFAGDSGDGMQLTGTQFSNNTALLGIDLATF
                     PDFPAEIRAPQGSLPGVSGYQLRFSSDRIFTPGDACDVLVAMNAAALKVNLKALKKGG
                     KIIVNTDGFDAKNLRLANYPEGVNPLEDGSLGNYEVIKMDVTKMTREALKDITMGMKE
                     KDRAKNMFVLGFLYWMYNRDMENTINFLRDKFGKKPEILESNIKVLQAGYNYGDTTET
                     FTTTYTVEKAKMESGTYRSIMGNQAVAYGLIAASQKSGLQLFLGSYPITPASDILHDL
                     SKYKNFGVKTFQAEDEIAAIASAIGASYGGALGVTTSSGPGIALKGEAMGLAVMLEIP
                     LVIINIQRGGPSTGLPTKTEQSDLMQAYYGRNGECPMPVIAASTPTDCFDTVYEAVRI
                     AVQHMTPVIFLSDGYIANGAEPWKFPQTKDLPEIKVSFKKQIDEGDEKYMPYKRDEKL
                     VRPWAIPGTPGLEHRIGGIEKQDGTGNVSYDPDNHQHMVKTRQAKVDLIANDIPEQKL
                     DSGPEKGKILVLGWGSTYGAIKSAVAELQSRGESVSHAHLRYIRPFPRNLGQMLKNFD
                     TILIPEINNGQLIKIIRDQFLVDAKGYNKVMGVPITKTELVMKLEELLGGLN"
     gene            31519..33453
                     /locus_tag="PK977_00670"
     CDS             31519..33453
                     /locus_tag="PK977_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRF16638.1"
                     /translation="MRRNLILLPVLLIPFLLFAQADEDIYEANRLKKVFPDDRVAATI
                     VEEEYNFDKGRSEDKLPVVTATKNTGITFLALRERASIQYFDFYNSFCKITSFKQLEK
                     MKGMFGIKKNYNVGYAIDRSASSSDFFSDDSRVKFFNIGFSGYGDATRVETEKKFFDS
                     KYLTSIYFHSWFPAKEKVVTIKVPDWLQVDIREFNFDGYKVTRQKGQEKGLTVYTFKM
                     QNLVPMKSEPRSIGVAYQYPHLILVVKSFNYDGKSEKGFGDVGDLYNWYKYLYNKCVN
                     KPDELKAKVTELTKGKTSDIDKVKSIFYWVQDNIRYIAFEDGLAGFIPATAQDVYKSK
                     YGDCKGMANLMTEMLKLAGLEAYMTWIGTRHIPYDYTLPSLAVDNHCISTVILNGKEY
                     FLDGTEKYIPFGDYAWRIQGKEVLIGKGDKYDVKKVPLMEIEKSKILTQTTFRLTDNV
                     LKGHVKVTLTGEQRTGFHQYYHDLPADDKKKMIQRFLEFGNKNLTVNNVKTSDLNNRE
                     IPVVIEGDIDLSNYIITEDKEVYVGIDFFPEDLRGVIPDTKRQQDYALHSAYVSQDET
                     ELILPAGYKVVSMPAAINEKSDDYEVSGSYTAKDNKVIFKKTLSFKTGRIRKADFENW
                     KNFTKKLKDFNSNLILIKKP"
     gene            33494..33877
                     /locus_tag="PK977_00675"
     CDS             33494..33877
                     /locus_tag="PK977_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRF16639.1"
                     /translation="MTENELATIAVDIAFRVHTALGPGLLESVYESAFAYEPDKKGIA
                     YTRQEEIPAKYEDIKLDVGFRADIIIENKLLIELKSIEAIEKVHHKTVLTYLRLADLK
                     LGLLINFGTNLIKDGIYRKINGQLD"
     gene            34094..>35431
                     /locus_tag="PK977_00680"
     CDS             34094..>35431
                     /locus_tag="PK977_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16640.1"
                     /translation="MKKLALILSSLFITATLLAQDDDTFSPSKLESIAKNMKPVWDDP
                     DADFAVSAIPEKWKEESGVIIAQKTRFSFDKDANKLAVYEITRRRIKLNDRDAVNTYS
                     SVYFRIGSSSDGAGIKIIKANGTVQDVSLRNAVYVEDNSDVPSTFTPYIGKASTYYDK
                     SKSRVIFYKVAVPDLDPGDIIDYGTIFYDDNTVKKMSFIEFDPIYFVCTREYPVISQK
                     FEIDTDDKSFVNFKSTMGAPLFKETGNSNADYLWEDRNREKLSDTRWVNRFVELPMVK
                     FQIVFSRSENRSDLFIGDKGELKQNISPAELAKKMNILYNKLDGSYYYSMAKAYLKQI
                     GYADMREDDFIQKTYYILRHMSHYKANGFSSELFASCFTQCLDLRKIPYELIVTSPNN
                     LTRPENIIFRTEPEWIVKVKDKFVFNATVFSNPFEFREEFLNTPAYIISLGKNP"
     assembly_gap    35432..35629
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35630..>35983
                     /locus_tag="PK977_00685"
     CDS             <35630..>35983
                     /locus_tag="PK977_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16641.1"
                     /translation="DDLRGGMKGDALEQYEERLRERKKEDKTRKLEYMKDALRDDFDN
                     ITVYNEFTLTADGRTWRKQELNYTNKYQLSDMVKIAGDNLLVSIPGLIGEQLFVSADE
                     RKREVDAYMGFPRSLR"
     assembly_gap    35984..35993
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35994..36242
                     /locus_tag="PK977_00690"
     CDS             <35994..36242
                     /locus_tag="PK977_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16642.1"
                     /translation="VISFTIPDGYKAVGIQNLNMNIDNETGTFAVQASVEGNTLSILV
                     KKLYKQVTVKKEDWPKLLEMLDAAFNFSQKKILLKKIN"
     gene            complement(36243..>36565)
                     /locus_tag="PK977_00695"
     CDS             complement(36243..>36565)
                     /locus_tag="PK977_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16643.1"
                     /translation="LINIRHSRHKYLLIISVYLCQAIATMRFSISIKYYNPGLQIGIE
                     DIKNAWYLPEGAAAVKLNTIVYQGQLTYRFPSSGKRISYRQLKKGLIKKRLIIRLPLE
                     LLPF"
BASE COUNT        10554 a         7915 c         7405 g        10230 t
ORIGIN      
        1 aaaaaacatc caggtcaatg gtacggttac cacctccaaa atctgctttg taatgagagt
       61 agttaccgct caggttcaca aatgccgtat aggctttgta cataaaagga taactaatat
      121 tcagtgcaaa cacatcctgc gtggcaagat ttctcttggt catgaaactc tttgatttgt
      181 cagtagtatc taccagctgt gtgaatacgt ctgacacatg actatagctc agtgaagtat
      241 tcaacttgaa tttatacgta ttggtcaggg taataatgtt agtatactgt ggcctgagca
      301 atgtattacc tttctgaaag gtatactcat ccagcttgaa ctcgaaagga ttaaggtcct
      361 ggtatgccgg cctgtcgatg cggcggctgt atgaaagtcc ccactggttt ttggggttct
      421 tgttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtatacttcc tttcaaaagt
      481 ttgcttactt cccttattta tagagccatc tccattcaag ccatatgaat cacctctcga
      541 attggtattc tccatcctta agcccacctg gaacattacc ccttttaatt gcttgttgta
      601 attaacatat aaggcattca cattttctgt ataatcaaaa tgattgcttc ttggaatatc
      661 cagttgtttg acttcaggat ttaactgggt gacattgtag cggccaaaat tattattggt
      721 attaataaat gctgttttga atccaacacc aaggcgacct tttttatagt actgctcata
      781 atctccttta aaagtataaa tatcaatatc ggtaggggct acaaaacggt agatgaattg
      841 attcaggatg gtagtaccag aagcattcag ataagtattg ggctgcagct ggttactttt
      901 caaacgaaac agcccgtaat cagcatccag gttcagttca cggcctgccg tgtcagcata
      961 gcgatagttc aggttaaagt tcaaattggt acggtccatc tggttgcggt tatttacatc
     1021 caataacctg tctgttacac ctgtaggctt gtaggtaatg ggtgttctgc catcattagc
     1081 catgttagtt tcagcaatgt ttccgttgat taaaacacca agagtacttt tcttgctgac
     1141 gaaataatca agtccggttt taaagccatg gctgttgttc ttattaacca ttttactcct
     1201 gccatcaaaa atagtatcca gttgaaccct gtacaggtta aacctgtttt cattcaggtt
     1261 atgattataa ttgtaattgc caaaaacatt aatgctttta ttacggtggt tcagactaag
     1321 tcccccgttg tatttaccat aaataccaat attgtaccct gcgttcaccg aaccattggt
     1381 accaaatgac ttattttttt tcagccggat attgataata ccggcattac ctgccgcatc
     1441 atatttggca gatggattag taattatctc tatagcctca atctgtgagg actggagtga
     1501 tttgagataa ttcgtaaggt ctgtgccgga taaagggctt ggctttccat caatataaac
     1561 ctgcacacca ttttttccgg caagactcag gttctcatct ttatctacca gcacacccgg
     1621 agattttcgc agtaactcca atgcatcatt acctgtagca ttgatagtac cctctacgtt
     1681 taggnnnnnn nnnnnnnnnn nnnncttaac ctcaattatg ggcttttgag ctgtaatagt
     1741 aactccctgc atttctttag ctattttctt tagggtgacg ggatcaagta gcagctcgcc
     1801 attaagctca aattgactag agtaataagt agtgtaccca atatgtgagg cactgataag
     1861 gtaacgaccc tgtgtggctt ctatggaaaa ttttccatca ttaccggtag tagaaaattt
     1921 gattacagac gaatcttttg tacgcaatag ggagatcgtc gttttatcga gccctttgcc
     1981 ctgttcatca attacaattc ctgataattt ctgagagttg gctgaaaaag aataaaggat
     2041 aaaggttccc aataccacaa ttaactgtct catttttaat ggtttttgct ttgaaccggc
     2101 aaagataccc cagtgtcata gaattgtaag catgtataca tacaaatggt gaaaattccg
     2161 gcatgaacgg ctacgccttt aataaaatct gtaaaactgt tgcattacaa atcaattgta
     2221 aatcatcatt ttcaccgtag gtttgcaccc aaaagaaacc atatgaattt ccctgcaaat
     2281 ctccgctaca ccaaagacca tgaatggata agccttgatg gcaacattgc aactattgga
     2341 attactgatt atgctcagag agaactggga gatattgtat atgtagaggt tgataccatc
     2401 ggaaagtcac tggaagccgg agctgttttc ggcactgttg aagccgtaaa aaccgtatca
     2461 gatctgtttc tgcccgttag cggaactgtt actgaactaa atccggattt agcaggctca
     2521 cctgagtccg tcaataatga tccctatggt gccggatgga tggttaaaat gaccgttaat
     2581 aacccagctg atgtagaagc attgcttgat gctgctgctt acgaagcgat ggtgggctaa
     2641 atccattttt tttgcagatg cgtatatagc aaagatgcaa tcatttattg tcattgtatc
     2701 ttaaccaatt aattgaacct caacactaca tacagcgaac aggagctggt ggacttgttg
     2761 cagcatcgca atgagaaagc tttcacttat ttgtacgaca attactccgg tgcactacta
     2821 ggcatcgtga atgcaattgt aacagataaa gaaacggcta atgatgtatt gcaaaatgtc
     2881 tttataaata tttggcggag gattgaatct tatgatcctt ccaagggaag attgtttacc
     2941 tggatgctga atatagccag gaatgcagct attgatgagg taagatccaa gggatataat
     3001 aattcgcaaa aaaatgttcc tttcccagaa tccaatgaaa tcgccggagc agtaaccggt
     3061 acagatataa ctgatgtagg cttgaaaaaa atactgggga acctcaaagg tgaactaagg
     3121 gtgctggtag atatgtccta tttccagggt tttacccatg aagaaatatc caaagcgctg
     3181 aacttacctt taggtactgt aaaaacaagg atcagaactg cattaattca attaagaacc
     3241 atgattcaat aaaaaagtga acgtacagga atacatatca agcggaattg tcgagagcta
     3301 cgtactcgga ctggcttctg aagaagaaag aaaggagttt gagcagtatt gccaacaata
     3361 tcctgaagtg atgaaagcaa gaactgattt tgaactggtt cttgaaaaac aggctatgca
     3421 aaatatcatt gtgcctgatc cttcacttaa atcaagaatt tggaaccaga ttggcgtaac
     3481 aggaaaagta ttgccaatgg ttgaaccggt accggtaaga aaaatgtctt ggataaagta
     3541 tgtggcggcg gcctctgtta ttttattgat tgttagttta tactggaata tctctctctt
     3601 taataaaaac caggagctca aaaacaatta tgatctggcc tcagaaaaga taaaggaaat
     3661 ggagcatgac aagcaggtat tgcaacaaaa cccccatgtg aaaatggcct cattgaaggg
     3721 tatggaagca tctccccagt cttttgctac agtttactgg gatacagcct ctcatgatgt
     3781 ttatcttctg gccaataacc ttcctgtacc ccctactgac aagcagtacc agctttgggc
     3841 tttattggat ggccaaccta ttgatctcgg tatgcttgat tattatgtca agcaaaagaa
     3901 gctgcttgtg agaatgaaaa atgcccagaa ggcacaagct tttgctatta ccctggagaa
     3961 aaaagatcgc agtgatgtat ccaagccaca gggtgatatg tatgtaatgg gtaatctctg
     4021 atcaactctc ctccatcaat ctgttcgctt tattatttcg tatttaatcg ggtatgacct
     4081 aagctatagt agtagttttg gttctatagt tggttaaaaa caaaagggcc tctttccagg
     4141 ggctcttttg gtattcagtg ctttacagta ttattgccga tattatatca aatgataaaa
     4201 ccttcactat tgccggcagc tggctggctg attctttgta ctatactgct agtcttgccg
     4261 ggatccgctt ttcctaaaga agactggctc agtaaaatat ggtttgataa atgggtacat
     4321 atcggcctct tcagcactat ggtggtattg tggtgttggg gggggcagaa aaaattcaaa
     4381 aagaagtata ttcttgttac aactgcagca ttagtatatg gcatagctat ggaatttata
     4441 cagcaatact ttgttgtgaa caggtcattt gatacaggag atattgttgc ggatgcaata
     4501 ggttgtttaa ttggttacgg atttagctgg aagtatataa aaaaataaac ccctgtagaa
     4561 acaggggtcg caaccaaaac taactgctta tgagaaaatt gactgctttt ataatgaggt
     4621 atgttattct attagtataa cgcaaattta ctgccggtag tatgatgttt gctgttaatg
     4681 aaagtttaaa agaactaatc cggtatttcc acttactctt cgttcgtaaa acctcttctt
     4741 cactttgtta tccttacgat gctgaatctt acttcattac atacaacaac gcttagcttt
     4801 tttagttgat tagttaccgt gaatttgcct tattatgact aagtattgac aatcaagtga
     4861 caaagaacta aacaactatt agacggttcg ttgccaattt actttcaaaa ttttgtgcca
     4921 aaaattatca atgaaggcaa atcatttgta aagaggaacc ctgattggaa atgctctgtg
     4981 tattaattca aaaaactctt aatcatatcc atcaaacccc cgctttgctg acggttttga
     5041 aagccgcttt gggctttctg tgtcagatta ctgataatat cctgcaaatc aaggccacca
     5101 ccagatccgg tgccaccacc tgtgaactga tttaagaggt tttgtaaaga aaagccgcta
     5161 ttgttttcct ggtctgaact gatggcatta cctccggtga gtgatcccac caggttgtca
     5221 agagtagcat tgggattctc aggatcagcg gtttggttta ttaaaccact aagtgcattg
     5281 ggaataaggt tgttagctac attacttgct gaagcaggac tcattctata tttattcacc
     5341 agtttactaa tgaagtaccc tatcatcatt gtaacaatag gatttttgac caggtttcca
     5401 attccggaac cactggtgtt atttgcacct cctttgaaca gatcaaggat gttttcaaat
     5461 cctccacccg ccattacatt ttgaaatcca ttggtaatag tgctggtggc atcggccaac
     5521 acatcctgat tatactcatt gggtatttca gggttattca ctactgtttc ttgtccaaat
     5581 tgctttacaa ggtttactat ctgatctaac ataatacaat gatttannnn nnnnnnnnta
     5641 aaattagcat atcccttctt gagaatggaa ttagtttctg atgagaaaaa gtacccggga
     5701 agagtcttag ttctgacgag ttagtttttc tgcattttca gcgaactgca gggcttcgat
     5761 cagttcttgt atgtcgccat tcagcacagc atcaagattg taaagtgtaa gaccgatcct
     5821 gtgatcggtt acccgtccct gcggaaaatt ataggttctg atcttagcac ttcggtcacc
     5881 ggtgcttaca agactcttgc ggtgacgagc aatttcatct tcctgctttc taacttgttc
     5941 ttcatacagt ttagttctta gcatctgcat ggctttctcc cggttgccta actgagatcg
     6001 ttctgtttga caaaccacta ccgttccggt cggtatatgt gtcagcatta ccttggtttc
     6061 taccttattt acgttctgac caccggcgcc accgcttcgg gctgtttcca tttttacatc
     6121 tgccgggttt agttcaaaat ccacttcctc ggcttcaggc attaccgcca cagtagctgc
     6181 agaagtatgt acccttccgg aagcctcagt atccggcacc cgctgcacac ggtgtacacc
     6241 actttcaaac ttcagcgtac catacacatc gtcaccggta acttcgagtt gaacttcttt
     6301 gtatccgccc accgttcctt cattttcact caggatggct gttttccagc ctcttctttc
     6361 gcagtagcgc atatacatac gcaacaaatc tccggcaaac aggcttgctt catcaccccc
     6421 ggtgcctgct ctaatttcca ggattgcatt tttttcatcc tgaggatctt ttggaatcag
     6481 caactggcga ataaacgctt cctgcttttc ttttttttct tcaagagcgg gggcttccag
     6541 ttttgccagt tccctcattt cctcatcccc cccttccaat gcttctttat aaaattctat
     6601 gtcgtccagt atcttcttgt aatcattata cgccacaacg attttttcca ggctacggta
     6661 ttctttactg gtttcagcaa attttttgtt gttacctaca atctcaggat tggtaagggc
     6721 aatacccaac tggtcaaacc gggcttttat ggcttctaat ctgtctaaca tagtatttat
     6781 ctcggggcgc aaatttagcg atttggtttt tgagtgctga aatttgaatt ttgagtatga
     6841 actaccgaaa attcaaagca gaccggatat ttgatggtta ccagtttcac caggggaaag
     6901 tattgatcat tgatgaagat ggaatttttg acaatatcgt ttccgaagaa gaggccggcg
     6961 aaaatgtaca gcagtttcaa ggtattctgg cgccagggct tattaattgc cattgccacc
     7021 tggaactcag ccatcttaaa gatgtaatac cacctcatac aggcctgata gagtttttat
     7081 gttcggtagt aacaaaaagg gatttttccc ctgaagtaat tcaagaggcc attacagctg
     7141 cagaaaaaga aatgtatgat aatggcatcg tagccgtggg tgatattggc aatacggctg
     7201 acacagttga tgtgaaaagg aaaagccaga tacgctggca aaactttgtg gaagtactga
     7261 gttttacgga tgaaaaagca gaggagaaca tagaacatta cagaaaagta gctgcgacat
     7321 ttaatacggt tcatggttca tggatcatgg acaaccgtac atcactggtc ccccatgcac
     7381 cctactcaat aagcccaaaa acatttaaac taatcaatca gttaaccgcc ggacaaataa
     7441 tttctttcca caaccaggag caccctgcag aagatgaatt atacaaaaca ggcggtggcg
     7501 aataccttaa gctgtttaag atttttggcc tcgatcaatc tcccttcccg gttaccggaa
     7561 aaagcagtat tcgttctgtt ctaccacatt ttaataacgg ccaaaccatt tttttgatcc
     7621 ataatacttt tatgcctgaa gaagatgtaa cctgggccaa tgaatatgca acaacgaacg
     7681 gattaaaact ggtgtattgc ttatgcataa atgccaacct gtacattgag aataaagtcc
     7741 caccaataga tatactgatg aaacataact gccagattgt tttgggaacc gacagttata
     7801 gcagtaactg gcagttgagc attgccaaag aaatagaagc aatcaggaaa cattttccgc
     7861 atataccgga agaaaccatt ttgcaatggg cgacaattaa tggagccaaa gcattacaat
     7921 gggaaaaaaa gttgggtagt tttgaaaaag ggaagaagcc gggcatttct ttaataaaca
     7981 tgggttattc catctcaaca aaattatcct agcacctctc tcaataccgg caaacttttc
     8041 attgttccaa aatcctggat atgcagcgca taatacgttt cataccggtg cagcaactgt
     8101 ctgcggaatt catgattaag cttgatgtca tccagttctt ccggctgcat tactttcaag
     8161 agttgtgaac taacagccga ttgtttttct tccaggaaat gcggatgagt gggttgaata
     8221 gcagtgaaac ctccttcctg cagatcaaga aagtgatacc tttcgctgta gttgtcagct
     8281 atacgaaacc caaaatgccc cggcaggtga agtgcaaaaa ataagggaag gtttgccgtt
     8341 attgttcctg aactgttatc caggtataaa aaacagtctt ctacaaaatg aaacaggtcg
     8401 ggattgcttt cgggctgttt taaacatttg gtcagcaatt ctaccataaa taaagccact
     8461 gcatttttgg ggacatcagt caaaacatgc cggtagagta ttgaccactt aaactctttt
     8521 atgcgattga gctgctttag ttcgttgtga taaaccacca ggtcaagaat agcagccggc
     8581 tgaaacatat tggccttacc ggttcctttt ttagaagatg ccctgacccc attaactaaa
     8641 taagattgta tgccaaataa ctctgtaaat agggtaacaa taacacttgt ttctccatac
     8701 ttcactgttc ttaatacaat gccttttgtc ttatgtagtt tatccggcat tatttgctga
     8761 taaagaaaat ttttgtggca gttctttcct tccggccatc atctgaaacc aataccaggt
     8821 aagcgccact ggaaattctt tgccctttat aatttcttcc atcccaaaca gcttgcccac
     8881 ctaatgctct tgtctggtaa actaatcttc cgtccagctc agtgattttg acaattgcat
     8941 tattcactaa tccacgtata gcaatggtgc cgctgaatcc cggtggcacc gggttaggga
     9001 acaccagtac attctcattg gcttcaccac cttcggtagc agtgctgcga aaagagcaaa
     9061 ttccttttga tgtagcaaaa aacacttcgc cggttctgcc gtctattgct attttcttta
     9121 catcactgct gagcagagga ctgtttgttt ctgtgaattg ataaatcact ttctcaccgg
     9181 aagcactaac caggaatact ccattgcggg tagccatcca tttcctgtcg gctccatccg
     9241 ccgctatact tctcacttct tgccctttaa aaagataacc tgcaaaattc ccattaggca
     9301 caatgggcca tacggcatca caggcgggtg aactgaaggc cagttccgga cattgaagca
     9361 ctgccacccc atcatttgtg ccgatccaga tataaccatt cttatccttt gtcacacaca
     9421 ccacatcgtt ggagggtaga tttccgttac ccgttccggt cccatacctg cgccatttat
     9481 caccattggt atcatcaacc gaagcaccat gatcataaca aatcaacccg ttcccttttg
     9541 gagatactat ccacttataa ttattatcat ctataacaat ctgtgataat gcattttcaa
     9601 aaacattaaa tggtaatgaa aaannnnnnn nnncagctgc cgtcagcttt tcttacccgc
     9661 agtggttgtg aagcgccgta gttggatatc cataaattat tatcattgtc aaacgccagc
     9721 cctgaaacac ggtagctaag cggatcacca accacagcac ttaggttgtt ttgtttaaag
     9781 atttcaaaag aagggcccgg cttaatatgt aataatccgc ctccgtaaga gccggcccat
     9841 actgtttcat ctcttttatc aacagcgatg cagataaaat caagcaacga atccaattga
     9901 ctgaacctgt aacgattggt atttgcccaa ctgccttctt taaaagaaaa aactccatca
     9961 ccattataga ggtaattcca tgcatcgttt accgatccgg ctgttgcata aaagacattc
    10021 tggtaaacaa gcatttctcc actggcgatt ccctgtggtg aattcagcat gtattgctca
    10081 taggcagatg atgatacttc aaacctggac aggcaggcaa actgatctgc cacccatggt
    10141 tgactattga ctaaaattgc tttacgggga aacgaaaccg ctcctgtgtt tgccagtaag
    10201 cgattgatgg tgccatctgg atttagtatc accacccggc tgaccccgct gatttgtctt
    10261 tggcacagca gtattttgtt ttccgcaaca gatgagctaa cccaaggcca tccatctgta
    10321 tacagcaaag accagttact gccattttgc ataaacagtg aatcgttttt ttgaacgatt
    10381 accctgtttt gtattgtaaa aacattttta catggtcccg ctgacaatcc gcctgttccg
    10441 cttattaatt gccagttggt atgattaccc ggggctggcg aattgatggt aattcgtttc
    10501 aagccctctt ctgttgcagc gtaataaaaa gttgcatcac tggtaaagcc atttactttt
    10561 acctggttgc cccctgcgcc aatgaaccag gaatctttta cctcgtagcg gttgccatca
    10621 atcacaataa ctcccaaacc tgtgctcagg aagaaattac cagaaaatga ataaatattg
    10681 tagacatttt tatccccgat gatattatct ctttttatat caggaatatt ataaatatca
    10741 ttccggtaaa tgatgtcaat attactgttg ctgtaagcaa taaagagttt ttcaccggcc
    10801 tcatcaaatc gaatagcgct gacacctgtt tcgcttaacc cggtaaccct actgtatctt
    10861 tcaattaagt tatcggtgag gctcacacta aagagactat acggagtagc gcaaaacagt
    10921 ttaccatttc cagcagttac atcaatggcg ctgttgtaag gcaggtgctc ccgccagaga
    10981 ccaaccggtg gtatcccgtc ctgtgccgat acattgatcg ttatcaataa tgaaataatg
    11041 aaaactatct tcctcaccct tcaaaagtat cacaaacagt catctccctt gtaagctttt
    11101 ttacctttct ttgcagcctt actgatacag attgttttat gtggcagagt ttaggtcgat
    11161 ttattttacg ttacaggttg ccgcttctcc tcatactggc aactattacg gcattcatgg
    11221 gttatcatgc cagtaaagta cagctgagtt atgattttgc cagggctatt cccattaata
    11281 accccaagta caaggtgtac caggaattca agaaaaagtt tggtgaagac ggaaacctgc
    11341 tggtactcgg catacaaaca gaaaaactgt ttgaagaaaa actgtttaac tcttacgccg
    11401 atctgcagcg taaactgaaa acagtaaaag gtgttgatga tatcatctct gttccaactg
    11461 cggtaaacct ggttaaacta cctgaaacag aaaaactaaa agctgaaagc atttttcctg
    11521 acagaaaatt gtcgcaagca gagatagata gtgcggctgc tacctttctg aaccttccct
    11581 tttaccggca attactctac aatcccaaca cgaatgcatg gttgatggga atccgcatca
    11641 ataaagacct gatggcttcc aaagagagat tgagtattgt gcataccatc cggcaaatag
    11701 ccgatgattt tggcaaagcc aataatataa ccgtatataa aagtggttta ccacaaatcc
    11761 gtacggaatt gacagaacgc attactaaag aaatgcggtt attcattttt gcatcgctta
    11821 ttttatctgc cgtcattctt ttactgttct tccgttcatt cagcgctatg tggctttcac
    11881 tgatcgttgt tttcataggg gtgatctgga gttttgccac catacagtta tttggctata
    11941 agataagtgt actgaatgca ttgattccac cattgattgt ggtgattgga atccccaact
    12001 gtatttattt tcttaacaag tttcatactt cctacaatga aaccggcgac aagaaagctt
    12061 cattggtgat gatggtagac cggnnnnnnn nnnnnnnnnn nnacgctttt ttgtaacctg
    12121 gctgccgcca ttgggtttgc agtttttgca ttaacccaga gccagatact gaaagagttt
    12181 ggggttgtag ccggcatcaa tatcctggcc ctgttcttta tatcactggt atttattccg
    12241 gtagtgctca gctatctgcc ggcacccaag agccgccata ccaaatacct gacaaatcca
    12301 aggctcaacc gctggcttga caggttggaa agatggtcat tgaatcatcg caaactgatt
    12361 tatggtatat cgatggcagt ggtggctgtt tctgttgccg gaattttcca gttaaaaacc
    12421 ctcgggttta ttgtggatga cctgcctaaa gaggataaac tctacaccga cctgcgtttc
    12481 tttgagaaaa acttcaaagg tgtaatgcca ttggagatat tggtggatac caaaaagaaa
    12541 tatggggtga cccgtaacct gaacaacctt cagcgaatag actcactgtc acaatatctt
    12601 ggcagcatgc cggatatagg ccgacctttg agcattacgg acggattgaa atttgccaac
    12661 caggttttta acgatggcga taccagctat tacagtatgc ctagtgagta tgctttacca
    12721 ggattgtcag gctacctgag tccaaaaggg gatagtgccg gcaacaaaaa ctccttttcc
    12781 aagctggtgg ccactttcat ggattcatcc cgacaacaag cccggatcag tgtcagcatg
    12841 gctgatgtag gtagtaaaag gcttcctttg atactggata gtatcgaaaa ccgggtcaac
    12901 caactatttg ataaggaaaa atatgaggtc aaaatgaccg ggaccagtgt taccttcctg
    12961 gaaggaagcg gttatatcat taatggttta aaagacagta ttatctgggc ttttgtcctg
    13021 atagcatttt gcatgcttta cctgttccgg tctgcccgta tcttgttgat ctcccttatc
    13081 cccaatatca tcccgctgct aatcactgca ggcattatgg gctgggccaa tattccgctg
    13141 aagccctcta ctgtattggt tttcagcgta gcattgggta ttgcaatcga catcactata
    13201 cgcttcctcg taaactacaa gcaggagctc ccgctgaata attttgatat gaaacagacg
    13261 gttattgaga ccattcacag caccggtatc agcatcattt atacatccct ggtactgata
    13321 gcggggtttg tcattttctg cttcagcggc ttcgacggaa ccaaggccct tggatggctt
    13381 acttcgctta ccttggttac cgccacattt accaacctgg tactgctgcc cgccattctt
    13441 atttccttta tcaaaatgag gaaataaggc catttacagt acatttgtta agcagctttg
    13501 gacggcggaa atttgtctgg aaattttaaa gcttttactg acggttaggg ctgataatca
    13561 ccgggaagac ggtggtttca aaaactgaac atttttttga atttttcgtt gaaggttaat
    13621 taatttcaag cccccttttg gagggttttc gatctctttt atatactaaa actaaacgca
    13681 catgagaaaa ctgtttttac tgctctttgg cgttgtgttt tttgccgctt cggcgttagc
    13741 acaacggacc atcacaggta aggttactga tgaaaaaggt gctgctattg caaatgcatc
    13801 ggtcatcgta aagggtacca ctaccggcac tactacgaag gccgacggaa cattcagcct
    13861 tcccatacct gccaatgcaa gagtattagt cgtttcatcc gtagacatgc tcacccagga
    13921 gttgaacatc agctcccaaa cttcttttga agttgtcctg aaaggcgagg ataaagtgat
    13981 gcaggaagta gtggtggtgg gttatggtac ccagcaaaag aaagccttta ccggttccgc
    14041 ttctaaggta gatgcaaaag agtttgccaa tcttatcact ccttcaattg ataagcagct
    14101 ggcaggccgt gccactggtg tgcaggtaac caaccagggt ggccttgtta acactcccgc
    14161 aaggatattt atccgtggta ttaactctat taataaccgc actcagcccc tttttgtagt
    14221 tgatggtgta cccataattg atggcaacct tgccgctact accaattcca atgccctcgg
    14281 tgatattaac cccgctgata ttgaaaatat cgaagtattg aaagatggtt ctgctactgc
    14341 catctttgga tctagggccg ctgccggtgt agtgctcatc actaccaaaa aaggtatcaa
    14401 gggaaggtca aaagtgacat acgatatgtc gcttggtttc agcaatgtgc ttaaaagatt
    14461 tgatttgctg aacgcagaag agtttgtagt gatctccaat gaaaaatttg ccaatgcagg
    14521 tgtagctccc cgcgccggcg taaatcccgg tggtgtaaat actgactggt taagcgaatc
    14581 cctgattgat aatgcactgg tgcaaaacca taacctgagt ttccaaggtg gtacagccaa
    14641 aaccactttt ttcttctcgg ccaactactc taaacagcag ggtgtggtta aaacaaacca
    14701 aaacatagct tacaggttaa gaatgaacct ggagcatgaa atgaacagtt ttatcaaatt
    14761 tggcaacaac ctgacagtaa cccgccaggt agatgccgat caaaataacg gcgccaatgc
    14821 actgagtggt tccattgcct cagcgcttcg tttgttaccc aatgtggcag cctataaccc
    14881 gcttcatcct accggatata atatcaattt ccccaacgcc aactctattc cgctgggccc
    14941 caactcagcc agtgtggatg ataattttac caatgtggcg tttactttgg gaaccaataa
    15001 attcacatct gacaaataca ggattatcaa taatgccttt atggaactgt ctttcacaaa
    15061 gcggttgaaa ttccgttctg tgggatcagc agatttcttt aatgattaca gttttcaaag
    15121 tctcgacccc cgtcatggtg atggctttgg ctccaatggt gttgccttta accaaaacca
    15181 aaaccagttg cgatatgtat ggcaaaacta ttttaactat aatctgaccg gaaaaaaaca
    15241 cagcctttac ctgacagcag gacatgaagt gcaaaagcag acgtataaaa cactttcagc
    15301 acagggaacc agtattgcag atttttactt cttaaaagag aacctgattt ctggttcagc
    15361 ctctaaccag tttgtaggtg gtggctatgc aaaagaaggt tttgaatcgc ttttcggaag
    15421 aatcaattat gattttaaga acaaatattt ctttcaggct tctatacgcc gcgatggtca
    15481 aagctccctg gcaaaagata aacgctacgg taacttcccc ggctattcct ttggctggag
    15541 ggtatcagaa gaaggtgcat ggaaggacaa caggttcctg agtaaatggt tccctgagtt
    15601 caaattgaaa tactcttacg caaaagtggg taatactctc accggcttca ggtatcttcc
    15661 tgctactttt ggtcctactt tatatggtgg tatcaacgcc attggtctca acttcatcgg
    15721 caaccccgac ctactttggg aaacaagtac aaagtttgat tttggcgctg aaatcggaat
    15781 gctcaacggc cgcatcaatc ttacgtttga ttggttcaaa aatgatgttg acaatttagt
    15841 attgaatgta cctacacctg ccagtgccgg tattccgggt aattcaattt cgcaaaacat
    15901 tggccagttg gtgaacaaag gattggaatt ctccattgat atagctgttt tcaaaaagaa
    15961 agattttaac tgggatgtta atttcaacta ttccaatgta aagaacaaaa taaccaagct
    16021 ttatgatatc aacggagtgc ctgttcctta cattcagaat ggcgcttaca acctgataca
    16081 ggttggtcag ccaattaatt tcatttgggg ttacaaatct gccggtgtca atacggccaa
    16141 cggaaacccc atgtactaca aagctgatgg ccgcctagta cagcataata tcgccaacaa
    16201 tacgttctac ttcatcagtg gccccaacga tggtaccatc aaccccgcca atgccactac
    16261 catgactatt ggcgacaggc agaacctggg ccagggtgtt ccggtttatt ttggcgcctt
    16321 caccaataat ttcaggtaca agcagtttga actggaagta atgttccgct actcaggcgg
    16381 aaacgagatt atgaatgtta cccgccagga agccttaatg agcaacagct tccacaacaa
    16441 tggtagggaa atattgcagc gctggcagaa acccggtgat gtaactaatg tgccgaggct
    16501 ccgctggggt cagagcaata acatcaacca gaatggtctg gctatttcaa gatttgtgga
    16561 aagcggtgat tatataaggc tgcaaaatgt ggtactgagc tacactttcg acaaaaacct
    16621 gatcagcagg gtatttaaag gctatgttga gaatattaaa ttcttcctgc aaggtcaaaa
    16681 ccttgcagta tggactgatt ataccggtgc agatcctgat aatattagtg ccggtggcgt
    16741 tgatgcagct gtggcgcctc agatcagaac catttctttc ggcctttcag ttggatttta
    16801 attaatgacc atcaaaaaat tatttgaaat gaaacaaata aaaaaatacc tggctttgtt
    16861 accgcttttt gccctcgggt taaccgggtg caaaaaagcc cttgacttag taccttataa
    16921 ctcggtagcc atagaagaag cttttagtac tccagctaag tgtcttgcct ctttgtatgg
    16981 cgtttatgat gctgcacaaa gtggtgccta catagatgga agcattcgtg gttatccttt
    17041 tggagctgcc aatatagagc agggtgattg ccgcggtgaa gatgtgatca atatcgcagc
    17101 cttcttccag attacttacc aggccactta caatgccaat agcgccaact gtgtaggcca
    17161 ctggaattct ttatatgccc tgataaaccg tgccaatgta agtattgacg gtttcagaca
    17221 ggccggaagc agcggtgtac tttctactgc tgtagctgca caatatgaag ctgaatgtcg
    17281 ttttctccgt gccctgtcac atcatgaact aatggtaatg tgggcccgtc cttacaggga
    17341 aaacccaaat ggtattggcg ttccttaccg cgactttgct gtaaaacttc cggctgatgt
    17401 agagctggtt cggactttac cccgctctat tgctgatacc gtttacaaaa gaatgttgag
    17461 agaccttgat tatgcagaaa tcaacctgcc tgttcagttt tccggaaatg atcgttcagt
    17521 gagggcttca agagctgctg ccattgcatt gaaaatgcgt atcaaattac atatgggcga
    17581 ttgggcaggt gtaataactg aaggtaacaa gctgatccct gctgttatca atccccttaa
    17641 cccatcatct gtagtcagtc ctattgggcc ttggtcactc acaccagcgc cggatggtcc
    17701 atttgccaat aatatttctt cagaaagtat tttctctgtc agaaataaca atattgataa
    17761 cactggagta aacggtgcat tggccgccat gctgggtgca gccaacctgg gtgcaagagg
    17821 tctggtagca gtttcaccca tcatttggaa taatccggga tggctttgtg atgatttaag
    17881 aagaagcctt ttgtatgtaa gcggtattaa tgccaataat ggtaccagca tctttactac
    17941 taaataccgt gattatgtaa accgcggtga ttttgcacct caaatccgct atgcagaagt
    18001 attgctgatg caggccgaag ctgagcaaag aaattcaggt agtgtatcta caagggcttt
    18061 agacctgttg aacactgtaa gaaaccgttc actggccact ccagcagcac agcagtatgt
    18121 agttggtaac tttgctactc ctactgacct gatcagggcg atattactgg agagaagaat
    18181 tgagttcctg ctggaaggaa aaagatgggg tgacatctct cgtttatcac aggatgctgc
    18241 tttcactact aatggaatcc ctgctaaagc aattaatggc gcatccggtg ctgctattta
    18301 taactgcggt ggcgcttata ccccgggcca ggccggtatc gcttacagcg acttccgctt
    18361 cctgtggcca atccctgcag aagagatcat tcagaacccg gttgttacac aaaaccccgg
    18421 ttactaatcc ctataagagt ttaatacaac aaaaagcccc ttcaaatgaa ggggcttttt
    18481 gttgcttgct attgacaagt aagctgctta tatacaagca acattatact gaaactctta
    18541 ttattaatca gcaatcattt gatgccaaat attgctaaca ctgtcagcta taaattcaac
    18601 aaccaggtga tgcattgtac aactaaatcc tccgcctcct atgcccatat tataattcca
    18661 tatttgtgaa gtaacatttc taagatcagg ttctccaatt aatgctttta cttcattgga
    18721 gtctttgcca gtaataatgc ctgattgaat aagatcgtca gccatcttat acctctcatt
    18781 tctctcagct atccatttat tccgtttgaa ttttgtttgc ttttcaaacg taatcatagt
    18841 aaagaccagc caaacaaaag cagcataagc aaacccggcg tatatcagtc tttttgcatt
    18901 cattttatgc gcctttgact tgcctgcttt gatcatcctc tttactaacc acctgcaaaa
    18961 aataattact acagcaaaag ctatcagcaa tttgacataa actgataagg gcaattgaag
    19021 aaatgctgtc accagggaac cattttaagt taaataaaca ccatcaatac ttttctgaaa
    19081 cacggatttt attgccataa ctgattagca catatatcat tgctgacatt ttggtattca
    19141 gcaatctttt gattgcccaa gattatcatt gaagaccaat tgaatccgtt tcatcagtgt
    19201 ccaaataaag atgcagttta aaaatacaat tgatattagc agccggttaa acagccctgt
    19261 taaaaatgac attaattatt gctggcaggg gcaaaaaaaa ccgggctgca gctgcagccc
    19321 ggctaattat ttatatgttc tattattcta tcagaatctc catcccaatg ttatgatagg
    19381 caatttgtct tcatttgaca tagcataggt agcgctgagt accagtttac ttagaggagc
    19441 aaaccagata ccaccgccat aaccataagc cagtttggta ttacccggat cattcttcac
    19501 ccatacttta cctgcatcaa agaaggtgaa tataccaata gcgccgggga agagataggt
    19561 agtgaaatca gccaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaact ttgacttgcc
    19621 ggcaaaacga taacggcgga aacccctcag gttgtcttcg ctgcccaggt attgggcctg
    19681 gtagaattca aatcctttgc tgcccacgtt ggttccacca ccaaagcggt tggcaaaaac
    19741 aaggcggtta ttattttttg acaggggtac ataaaagctg aaatcagaat tcatctgggt
    19801 aacagcatac ggattatcct tcagccctgc gaggtgacgg atattcgttt cccagtaaat
    19861 accctttgta ggcaaagccc tgttatcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnctgtatt aatatctagc ccattggccg gtacatctgt
    19981 tataaaacgc tggctgtttt tatcgtcggt tgaatccagt tcataacgct ggaaagtagg
    20041 accaaacgat acggtgaact ttggactaaa tctttccctc accaataaag aggcatctgc
    20101 caggttatac cttgcccggt aatacctgaa ttttcctgtt tgcgatttat cgtaaacaga
    20161 gttcatacca tagccaaaaa agttcgtagt gttattggga gcttttacat caatatcagt
    20221 aacaatgtcg gtcttggtac ccaatactcc gatgaactca ttgtnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnctggta gaaagagcat gacttgcagc aatggtatgc
    20341 gatgatttat agggttcctt gcggaatccg tgattgacaa acttgtaaga cagaccgaga
    20401 aacagtccat catcaggatt gaagcctacg gcaacacccg gaccggcttt atcatacttg
    20461 taataaatac gctggaatgt attaaccgta ctgtcattgg acatgcgatt ctttaccctg
    20521 cccttcactg tattcttgct ttctgctttg tcatatacaa aaagacttcc tcttgtccgg
    20581 ttggtatttt caaaataatc ttcatcaccg ccaccaatca ttctcacttt aatgcggtca
    20641 ttatctcctt ttactacaaa tttgtctgta ccatcaaagc cgtacaggcg gatctcccgg
    20701 gtttcgccgg ctttaaaagt ccgctcatac attttgctgc tttggttgcc ctccttatcg
    20761 attttatata cctggagcag gatatcaccg ttcgcattac gggttacatc aaaaaattct
    20821 tttttatcgc tgccggtaat acttacgatc tcagcaagaa agcgatagta atccattact
    20881 tcagcagcaa gataattgcg gcgctgcttt aatgtttctg caattttggg tccgttaata
    20941 tttcttatct cagcaggctg ctgggctata gctttatcaa tgacttcatc agtcatttta
    21001 gtaataaacc agtcaacagt tgttttccag tcctgttcgc taagtccgtt caggaaaaag
    21061 cggtcaaggt tacgggctgc aaaattgaaa tggcgaatgc tctttgcctg tgccttaaaa
    21121 ccctccagct ggggtacaaa cgatttttta ctggcaaaac ggggcaaggt tccctggttt
    21181 gtataaaatg cctggtccct gtcacggggt atggggaaat aggtttttcc gcctttgctg
    21241 ttctccttag ccccccactg ccattgatcc tcatggcggt caagatccat tacatacata
    21301 tccaggatac gggctaccag taaagctttc tggtcaactt cgttatcatt atccttttcc
    21361 agtttttcgg ctacttcgtc tgtatcatat ccttttgcca ccccttctgg caacctgtct
    21421 tcaaaagtgg ccaacatgtt gccaaactcc tggcgaaatt ctcccagcgc cggatcatcg
    21481 ggtatataaa ccnnnnnnnn nnnnnnnnnn ccataaggaa cacctactgc atcagccaac
    21541 ggctgcatgg ataatgaggc ataaggataa gaagccgaaa caccatcagc cacaaggtct
    21601 gcagccgctt cactatttag ccccccgggc agcgtttttg atgttatgaa tttctgaatg
    21661 gaccggattg tatattgctt tccgctggca tcctgcattc tcagggattt agtttgctta
    21721 cctcctcctc tttttacggg ggtaagacct gtcaggcttt gcaccggtac ttttacaggt
    21781 gtattccact cggtacggta attagcaccc atccatctca ttcgggcacg gttagtagtg
    21841 aacttgcccc ctgctgcagt agcagaatct tttccctgtt gggcatctgc aaatatggtg
    21901 gtggttgata ggaaggcgat cacaagtagt cgctgaagtc tcattgtctc aggttttggt
    21961 tgttaattgt ttgattaaga tatacgaatg agaaaagata cgatttttta aaaataaagc
    22021 aaattgtaat aatttattat tacaccgtta acagcagctt cacctgtacc cgccaatcct
    22081 gaagcaatca actaattttg gaacaagtgc tgttggcctt aaacttttct gtaaattgaa
    22141 aactttatat gggccgttct cgttataaag cggttcattt tcctgtcccc cacatcccct
    22201 aagggctgta aaaaatggtg aaccaggaag aaggcagatt atgatttgca acaaagggta
    22261 gtaatgagaa aaaaagtatt actgattggc tttttactgg ttcaggtatt ggtgaatgct
    22321 caaaaagaca gcattacgca acgaattatt ttaattggcg atgccggtca actgacaaac
    22381 ggtaagcacc ctgttgtaga tgccgtaaaa aagaatatcc cactcgataa taagaccact
    22441 gtcatttacc tcggtgataa tttatataaa agcggcctgc ctgatgtaga gtataaaaat
    22501 tatgatattg ccaaagctgt tttggattcc caaatatcaa ttgctgacgg aacaaaggcc
    22561 aaagtatata tgataccggg taaccatgac tgggagaatg gtaaaagagg cggttataat
    22621 gccattatcc ggcaacagtt gtatgttgat tttatcaaga agaaagaaga caatgtaaaa
    22681 tattaccccg aggatggttg tcccggccca gttaaagtcg atcttggcaa ggatgtaacc
    22741 ttgattcttt tcgacagcca gtggtggata catccgcatg aaaaaccaga aatagaatca
    22801 gactgcaact gcaaaacaaa agatgaactg gtaacacaaa taggagatat tgctgccaat
    22861 aattcaaaaa aattaatcat tcttgcctgc catcacccgt ttaaaagcaa cggcgcacac
    22921 ggcggatatt ttaccctgaa acagcacatc tttcctttta ctgatttgaa aagatcactg
    22981 ctaattcctt tgccggtcat tggttcagcc tatcctattg ccaggagtgt tttcggttct
    23041 ccccaggata tcagtcaccc ggcatatacc aacatgatca acagggtaac tgcagcattg
    23101 aaagctaatg cccccaatgt aatttttact tccggtcatg atcataacct gcaattgata
    23161 aaagaagatg gctataacta tgtggtgagc ggaggtggtt gcaaagaaaa ccgtacctca
    23221 aaaaacacga acagtttgtt taatacaacc tataatggct tttctgtttt ggaagttact
    23281 agtaataaag atgtcaatat taaattttac accgtaacag attctgtacg cctggcatac
    23341 acatctcatt tgctcaactt ttcgaagctg cctgaagaag ttgtactgca ggcagagcat
    23401 aaagatgatc cggcagctat aaggcttgac accatcagca aggcggcatc tgtacaatac
    23461 cagccggtaa gtggtttgaa gcaatatttc atggggcaga attatcgtcg tgaatggtca
    23521 gccccggtca atatgaaagt gcttcatttc gatacagaaa aaggaggact gaaaatagtg
    23581 agcctgggcg ggggaacaca aacccgttca ttgagactgg cagacaagag cggaaaagaa
    23641 tgggtgcttc gcacagtaaa aaaatattcc aaccaggcat ttgctgaaaa tgtaatggga
    23701 agcagccggg atcagtttaa acctgagatc agcacggcag cacaccccta cggatccttg
    23761 atagttcctg acctggccaa tgcactcaat ctgaaagttg caaaaccaga actattctat
    23821 gttcccaaag attcaattgc ccttggctta tatacaaagc tttttgcaaa caatgtttgc
    23881 atcctggagc caagaaactt taccgaagac ggatcggaaa caaaaacaac agcaaaaatt
    23941 ttcttcaaga atatgctcga ggataatgat catcgtgcag atcagctagc agtcttaaag
    24001 gcaagattgc ttgactttat tattggagac ttcgaccgac attttgatca atggagatgg
    24061 gccactattg acagtgccga catgaaagga aagatctatt acccggtgcc gagagacagg
    24121 gaccaggcat tttttaaccc gacaggaaag attttccggc tgataggtag ccgtgaaatg
    24181 ccatggttga aagggtttaa aaatgaaatc aaaaaagtaa actggctggg gtacacagca
    24241 agggattttg acaggatatt tttaaataac ctgacagata agcaatggaa aacagcagca
    24301 gatgaagtag tgaacaacct tagcgattca gttatccgca atgccgtaaa acaattgcct
    24361 cctgagatct ttgccataag cggggaagcc actattcaga aaatgataag ccgcaggaag
    24421 caactggaaa aagaagcgtt ggactactat gattttcttt ccaaagaagt gaatgtggtg
    24481 ggcagtaacc agaaagaata ttttaaagtg agcagtcatc cggaaggatt gcaggtaagg
    24541 gtttatgaaa aatcaagaaa gaacgacacc agtttcatta tgtacagccg tgtatttgat
    24601 ccggcagtaa ctaaagagat tcgtttattt gggttaaatg atgaagacgt ttttgaaatt
    24661 gaggagaatg tttcatcacc tatacgaata cgtatcatcg gcggccgtgg caatgacacc
    24721 tttaatatta aaggcaatgt gagaaatatt atttacgacc tcaactccga cctgaatttt
    24781 atcaaaagca gtaaccgcac ccggaaccgc ttttctgatg atcctccggc aaatgaaaga
    24841 agtattcttg gatttagtta taacactctt cagtttccgc aactgatgat gggttacaac
    24901 tacgatgatg gatttatgat aggtgcaggc atttcacgga gaacttacgg atttctgaat
    24961 ctgccctatg ccaccgatca gcagttttct gccatctatg cggttgaacg aaaagcatta
    25021 caactccgtt accgcggaga attcaatcaa attggcaaga aaactgacct gctgatcagg
    25081 gcaggtctga acactcctaa tatcaccaac tttacagggt ttggcaataa tacaaatgtt
    25141 ccggatggaa agaattttga cttttaccgg attcgctatc gtactgttga agctgagttg
    25201 ttgcttcgcc gccgaccctt tgaaagcctg cacctgatgg ccggtcctta tttttatcag
    25261 tacagtaata attatgctga taacaataaa actatccttg cagaaccaag acaagttggg
    25321 ctcgactctg ccgacatctt tggtaaaaag agctatgccg gcctcaaact ggccatgcag
    25381 tttgacaacc ggaataacct gttatttccc acaagaggtt tgttgtggac aaacgaaatt
    25441 atagccacag ctggtcttgg tgacaaagca gataatttca cccggttttc atccgatatg
    25501 acgatatacg taagtcagaa agatcctgca agagctgtac tggtactcgg ttttggtggc
    25561 agcaaagtgt tttcagatga ttatgaattt ttccaggcgg ccaatatcgg taataacaat
    25621 atcaatattc atggcttcag aaagnnnnnn nnnnnnnnnc aggttcctgg gaaaatcttc
    25681 catttatgcc agtgcagaag cccggataaa attattcaat attaacggct acctcctgcc
    25741 gggacaattt ggtattaccg gtttctatga tgccggaaga gtatggtacg ccggcgaatc
    25801 ttccaaaaga tggcatggcg cttatggcgg cggactttac ttcattcctt tcaaccggtt
    25861 tattatttct gctgccgcag gtttttcaga gaatgaaaga atgctgaact ttagcctggg
    25921 taccaaaatg aatatttctt tttgatcaat tctgcttcag catgacgaaa agactgatag
    25981 caggcactgc cctgcttaca ttgatttttg taatatggct ggtattcatt gccaacaata
    26041 caaggcctga tgaagttgta tttgacctgg tggcacctta caccaatggt gaaagaaccg
    26101 actggatgaa aaagatcagt tttttgggca accatcaatt cctgatccct gccaatcttt
    26161 tactgatcgc tttctttatt tctgtaaaaa aacataaaga tgccttattg gtagctatag
    26221 tggcattgag cagcctgggc ctgatgtctt tattaaagaa cctggttcag cgataccggc
    26281 cacctgctcc gctggtagat ggtattacca attttagctt tcccagtggt catgcattga
    26341 tgagtgtagc cttttatggc gtattggctt tttatgctgt aagaatgatc aataaccccg
    26401 taataaagat tacaatcctg cttttactat ttgccgttac gctgcttatt ggtttcagca
    26461 ggatctacct gcgggtgcat tatactaccg atgtaatagc cgggttcagc ataggcatat
    26521 gctggatgct attttgcttc tggctgctga ataaagctgc aaagcccggc aatcagggct
    26581 tatagacaaa ccgcaggatg gttgatcggg atgtaacccc ctcgttggag atatacatat
    26641 caccgtttcg tttaaatgtc aacccctccg gctgcggaaa taacttttct cccaactcag
    26701 aaacagattc aggattgcca ttcaggtcac aaatcaccaa ctggctgctg gcagaagaaa
    26761 tgataaataa tttattcagt atgggatgaa tccctgcagc cgatggctga aactttgatg
    26821 ttttcttatg gcctgcttct gttgctattt gctttatttt ggcagcatca atctggaata
    26881 ccgggttact tacaaaacca atagaatcgg gatagaaagc gaatgcactt accttcgact
    26941 tatcatccat gccacagttt ttgcaaacta taatcaatgc ctttctggcc gtatcagcat
    27001 acatggtctc aaaatcattg gtgccagtaa tattgagctt accagcctcc acgcctctta
    27061 caccggcact atcccgaatg atttttgtga tcataccatc gcttcgcagt acatagggca
    27121 caccattata gatagccaca tcttcataat ctcctttgcc accaaactta taatcggccg
    27181 taattacatt agtcgcctta tccagtacaa agaggtgacc tgactcatca taatgcgcca
    27241 gaaactcatc ccggtcagca tcccaggcta gcccggatat ttctttaagc tttaggtcaa
    27301 gctttactga ttgaacttcc gcaaaattgt aatgcggtgg cgacttgatt acccttttct
    27361 taggcttgca gccacttaag agcgcagcag ccatcaacaa gtatgtaaaa cgcttaatcc
    27421 tgaactttaa aaaagccatt cttctatttt tatttaaatt tatcaaaagt tatctttata
    27481 gcatggggga caaccattta tcgattttac aggaggccca ggcacttgta acagaccttt
    27541 tgctgaacaa actcagtaaa aatataaaat ttcacacatt acagcataca caggaagtag
    27601 tagcagcctg cgagaagatg gccgaccatt accaactggg ggccgatgac cgctttgcac
    27661 ttatgctcgc agcctggttt catgataccg gatataccag tggcgatgca aaaggacatg
    27721 aaaatgtcag cattgaaatt gccgatgaat tcatgagagc aaggcaactg gatgacgcca
    27781 ccattcaaaa agtgaaaggt tgcattggcg ctacccggat gccgcaaagc cctgccactg
    27841 ccattgaaaa aataatatgc gatgctgatc tttttcatct tggcactgat tcttttgatg
    27901 aaaagaaccg cctgttaaga caggaactga tcgattttgc cagtaatgat gtttccaaaa
    27961 aagactggcg aaagatcaat attcgtttcc ttgaaagaca tgagtacttt actgattatg
    28021 ccaaacaaaa attacaaccc gtaaaagata aacaccttgc tgaactgatc ggcaaagaag
    28081 aaaaaaacca accaaccatg aaaccagaaa aaaaagaaaa agacaaaaaa gataaggtga
    28141 aagatccggc agcagaagca gcagcagcgg aagccaagtt gaaggcccag aaagaaaagg
    28201 aagctgacag aagtatcgca accgtattcc gcatcatggc gcagagtcat tctaacctta
    28261 gcggcatggc tgactcaaaa gccaatattc ttatctctgt caattctatc attctttcaa
    28321 taatgatctc tacattattt gaaaaactgc agacagatcc taatctccag attccattgg
    28381 gcgttattac aattgtatgt gtcgcctcca tcgtttttgg cattctggca acaaggccca
    28441 atgttacaca cggtactttc accagggatg atattgccag caagaaaact aacctgttgt
    28501 ttttcggcaa ctatcacaac atgaatcttg aagattataa ctgggggatg acggagatgc
    28561 tgagcgataa aaactatatg aatgcaagca tgatcaaaga ccagtatttc ctcggtgtgg
    28621 tattggctaa aaagtacagg tatctacgca tcgcctataa tatttttatg tatggcctta
    28681 tactggctat gctgtccttt gtagtagcct tcttgcttcc gggttcaaca gaagtttata
    28741 cagcacctta acaatattca ttaacattaa acccatctta tcgtacaaat gaaaaagacg
    28801 cttatcggtt ttgcagctat tattttattt gcctgcaata attccaaaaa agatccggca
    28861 cctgaaacaa ataaccaggc tacagacgcc ccgccaacta caacaaccac agcgccggct
    28921 cctgctgcag caagtaccaa ctcagtcata acaatagaag gaaaagaact taacctcaac
    28981 ggcagcatcc ttgttgacaa agacaaaaag aaactgcagg ccggtgcacc ttaccgtggt
    29041 atgatcacca gcagcagcgg cccgaatgat gaaggctaca tactgcgttt cgtttttgat
    29101 accaagccgg gtgtatatcc cgtcacaggc atcagcttcg gccgcggtaa agatgataat
    29161 gcccagcagt ttggcggctt actcggtggt gaagaaaaaa tatatgacag caaggtaacc
    29221 ctgacagaat gtaaggatct tggtgataac ggggctggtg gtcacaagtg gagcatcagc
    29281 ggcaccgttg aaaatgtaac cattcccgct atgggaatta tgctgctgga taaagagaaa
    29341 aatcatccca aagagattaa aatagacaag cttagtttta ctggacttac atttgatgat
    29401 aatgctgaag agatgctgga gaaagctatg gaaatgctga agaaaaagaa ctaaagcaat
    29461 tgatgcataa actaaaaact ccggaaagaa tccggagttt ttttattgca tttgaatggg
    29521 taacaattaa ttcaaaccgc ccaataattc ttccagcttc atcaccagtt ctgtctttgt
    29581 aataggtaca cccatgacct tattatatcc tttagcatct accaggaact ggtcacgaat
    29641 tatttttatc agctgaccgt tattgatctc agggatcaga atggtatcaa agttcttcag
    29701 catttgcccg aggttacggg ggaacggtcg gatataacgc aggtgagcat ggctaactga
    29761 ttcacccctc gactgtaatt ctgctactgc agatttaata gcaccgtaag ttgagcccca
    29821 tcccagtacc agtatctttc ctttctccgg gccgctgtcc agtttttgtt caggaatatc
    29881 attggcaatc aggtccacct tcgcctgcct tgtctttacc atgtgctggt ggttatcagg
    29941 atcataactt acgttaccag taccgtcttg tttttcaata ccaccgatac gatgctccaa
    30001 acccggtgtg ccaggtatag cccatggcct tactaatttt tcatctcttt tataaggcat
    30061 gtatttttca tcaccttcgt ctatctgctt cttaaaggaa actttgatct caggcagatc
    30121 ctttgtttgc ggaaacttcc atggctctgc accgttggca atatagccat cactcaggaa
    30181 gatcacgggg gtcatatgct gaaccgcaat ccgcactgcc tcatacactg tatcaaaaca
    30241 atctgtcgga gtagacgcag caatcaccgg catagggcat tctccgttgc ggccatagta
    30301 cgcctgcatc aggtcgctct gctctgtttt ggttggcaaa ccggtagaag gaccacctct
    30361 ttgaatattg ataattacca atggaatttc aagcatcacg gcaagaccca tcgcttcccc
    30421 tttcagcgca atacccgggc cactggaagt agtaacaccc agtgcaccac cataagaagc
    30481 gccgatggcc gaagcaatgg cagcaatttc atcttctgcc tggaatgttt ttaccccaaa
    30541 gtttttatac ttgctcaggt catgcaaaat atctgatgcc ggggtaatag ggtaagaacc
    30601 gagaaataat tgcagaccgc ttttctgtga agctgcaatc aatccatacg ccactgcctg
    30661 gttacccatg atagagcgat aggtacctga ctccatcttt gccttctcca cagtgtatgt
    30721 agtggtgaat gtttcagtag tatcgccata gttataacca gcttgcaata ctttgatatt
    30781 gctctctaat atttcaggct tcttaccaaa cttatctctc aaaaaattaa tagtattctc
    30841 catatccctg ttgtacatcc aatacaggaa tccgagtaca aacatgttct tggccctgtc
    30901 cttttccttc atacccatgg taatatcttt cagggcttca cgggtcatct tggtcacatc
    30961 catcttgatc acttcataat taccaaggct gccatcttca agcggattta ctccttccgg
    31021 gtaattggca agacgcaggt tcttggcatc aaaaccatct gtattgacaa tgatcttacc
    31081 tcctttcttc agggctttca ggtttacttt caaagcggca gcattcatag ccaccagcac
    31141 atcacaggcg tcaccgggag taaagatgcg gtcgctggag aaacgcagct ggtaaccact
    31201 cacaccgggc agcgagccct gaggcgccct tatctccgcc gggaaatcgg gaaaagttgc
    31261 caggtcgatc cccaacaatg ctgtgttgtt tgaaaattgg gttccggtta actgcatgcc
    31321 atcgccacta tcgccggcaa acttgataac cacatcgctt aatacttcct gttttctggc
    31381 cattgcttat ttgcttattt aattctgcga atttaacaca agttgggctt gaaaagcaca
    31441 ttgattttgg tcatccgggg ccatatttta cctgagaaat atcagtaaat ttttatattg
    31501 ccagttataa ttatatctat gcgccgaaac ctgatcctgc tccccgtact tctcattcct
    31561 tttttactct ttgcacaggc cgatgaggat atttatgaag ccaatcgcct caaaaaggta
    31621 tttccggacg accgggttgc agctaccatt gtagaagaag aatacaactt cgataaaggc
    31681 aggagtgaag acaagctgcc cgtagtaact gctacaaaaa acaccggcat cacttttctg
    31741 gcactgcggg aaagggccag catccagtat tttgatttct acaactcttt ttgcaagatc
    31801 acttccttca aacagctgga aaagatgaag ggcatgtttg gcatcaagaa gaattataat
    31861 gtgggctatg ccatcgaccg ctcagccagt tccagcgatt ttttctctga tgacagccgg
    31921 gtcaagtttt tcaatatcgg cttctccggc tatggcgatg ccacccgggt ggaaacagaa
    31981 aagaaattct ttgacagtaa atacctcacc agtatctatt tccatagctg gttcccggct
    32041 aaggaaaaag tagtaactat caaggttccc gactggctgc aggtagacat ccgtgaattc
    32101 aatttcgacg gttataaagt cacccgccag aaaggacagg aaaaaggcct taccgtttac
    32161 acctttaaaa tgcagaacct ggttccgatg aaatcagagc cccgctcaat tggcgtagct
    32221 tatcaatatc cccacctcat ccttgttgta aagtcattta attatgatgg caaatccgaa
    32281 aaaggatttg gtgatgtagg cgatttatac aactggtaca aatacctcta taataaatgt
    32341 gtcaacaaac ccgatgaact caaagcaaaa gttaccgaac tcaccaaagg caaaaccagt
    32401 gacatcgata aagtaaaatc tattttttac tgggtgcagg ataatatccg ttacatcgct
    32461 tttgaagacg ggctggccgg ttttattccc gccacggcac aggatgttta caaatccaaa
    32521 tacggtgact gtaaaggcat ggccaacctg atgacggaaa tgctgaaact ggccggcctc
    32581 gaggcataca tgacatggat cggcacccgc cacattccat atgattatac cctaccctca
    32641 ctggcggtgg ataatcattg tatcagcact gttattctga acggaaaaga atacttcctc
    32701 gacggcactg aaaagtatat tccctttggt gattatgcct ggcgtattca gggcaaagaa
    32761 gtactgatcg gtaagggcga caagtacgat gtgaagaagg tgcctctgat ggaaattgaa
    32821 aaaagcaaga ttttaacgca aactactttc cgtcttacag acaatgtact caaagggcat
    32881 gtgaaagtga cactgaccgg ggaacaacgt accggttttc atcaatacta ccatgattta
    32941 ccggctgatg ataaaaagaa aatgatccag cgtttcctgg agtttggcaa caagaaccta
    33001 accgttaaca atgtaaaaac atctgacctg aataaccggg aaatacctgt agtgatagaa
    33061 ggagatattg atctcagcaa ttatatcatc accgaagaca aagaagtcta tgtcggtatc
    33121 gacttcttcc ccgaagacct gcggggagta atacctgata ccaaacgcca gcaggactat
    33181 gccttgcatt ctgcctatgt atcacaagat gaaacagaac tcatcctgcc cgcaggttat
    33241 aaggtagttt ccatgcccgc agccatcaat gaaaaatcag atgattatga agtaagcggt
    33301 tcctatacag ccaaagacaa caaagtcatc ttcaaaaaaa cgctttcatt caaaacagga
    33361 agaatacgca aagccgattt tgaaaactgg aaaaacttta caaagaagtt gaaagatttt
    33421 aatagcaacc tcatccttat taagaaacct tagtatttcc cacaaatcca ccaggaacac
    33481 aaagaaaaat gatatgacag aaaatgagtt ggccactata gctgttgaca tagcctttag
    33541 agttcatacg gctttgggac ccgggcttct ggagagtgtt tatgaatctg cttttgccta
    33601 tgagccggat aaaaaaggta ttgcctatac aaggcaggaa gagatacctg caaaatatga
    33661 ggacataaaa ttagatgtcg gctttcgggc cgatatcatc atcgaaaaca agttacttat
    33721 tgaacttaaa tcaatcgaag ctatagaaaa agtacatcac aaaacagttt tgacatacct
    33781 gagattagca gatttaaaac tcggattact cataaacttt ggtacaaatc tgataaagga
    33841 tggtatctac agaaaaataa atggacaatt agactaaaat catgaaatga ttttaccgtg
    33901 gccttaaaat agtaaactga cactcccagc ataaggaaaa caaatcagtg ccctttgtgc
    33961 ctttgtggga aacaaaaaag ctcttaaaaa tcacgaagtg atttcgccgt ggccttaaaa
    34021 atgaataaga ccacttcaaa caaagaaaac taaacagtgc tctttgtgcc tttgtgggaa
    34081 acaaaaaaat aatatgaaga aactagcatt aatactcagc agcctgttca tcacagcaac
    34141 cctgttggcc caggacgacg acaccttctc cccctccaaa ctcgagtcca tcgccaaaaa
    34201 catgaaaccc gtctgggacg atcccgatgc cgatttcgcc gtatccgcca ttcctgaaaa
    34261 atggaaagaa gaaagcggtg ttatcatcgc tcagaaaaca aggttctctt ttgacaaaga
    34321 tgccaacaaa ctcgccgtgt acgagatcac ccgccgccgc atcaagctca acgacaggga
    34381 tgcggtcaac acctattcat ccgtttactt ccgcatcggc tcttccagcg acggggcagg
    34441 catcaaaatc atcaaagcca acggcaccgt gcaggatgta tccctgcgca atgccgtata
    34501 tgtagaagac aacagcgatg tgccctccac cttcactccc tatattggca aagccagcac
    34561 ttactatgac aagagcaaga gcagggtcat cttttataaa gtagccgtgc ccgatctcga
    34621 tcccggtgat atcattgact acggcactat cttctacgat gataacacgg tcaagaagat
    34681 gagctttatc gaattcgatc ccatctactt tgtatgtaca agagaatatc ctgtcatcag
    34741 ccagaaattt gaaattgata ccgacgataa atcattcgtc aatttcaaat ccaccatggg
    34801 cgcaccgctc ttcaaagaaa caggcaacag caatgcagat tacctgtggg aagaccgcaa
    34861 ccgggaaaag ctcagcgata cccgttgggt caaccgcttt gtagaattac ccatggtcaa
    34921 gttccagatc gttttctccc gcagtgagaa ccgttccgat cttttcatag gtgataaagg
    34981 tgagctgaaa caaaacatca gtcccgcaga gctggcaaag aagatgaaca tcctctacaa
    35041 caagctggat ggttcttatt attactccat ggccaaagcc tatctcaagc aaattggcta
    35101 tgccgatatg cgggaagatg atttcattca aaaaacctat tacatccttc gccatatgtc
    35161 gcactacaag gccaatggtt tctccagcga actgtttgcc tcctgcttca cacaatgcct
    35221 cgacctgcgc aaaatacctt atgaactcat cgtcacctcg cccaacaatc ttacaaggcc
    35281 cgaaaacatc atcttccgta ccgaacccga gtggatcgta aaggtgaaag ataagttcgt
    35341 attcaatgct actgtcttct ccaatccctt cgagttcagg gaagagtttc tcaatacacc
    35401 ggcctatatc atttcattgg gaaaaaaccc annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgatctgcg
    35641 gggcggcatg aagggtgatg cactggagca atacgaagaa agattgcggg aaagaaaaaa
    35701 agaagacaag acccgcaaac tcgaatacat gaaagatgcc ctgcgggatg attttgacaa
    35761 cattaccgta tacaacgaat ttaccttaac agctgatggc cgcacctggc gcaagcagga
    35821 gctgaactat accaacaaat accagctcag tgatatggta aagattgccg gcgacaactt
    35881 actcgtttcc attcccggtc ttataggtga gcagttattc gtttccgcag atgaacgaaa
    35941 aagagaagta gatgcctaca tgggttttcc ccgcagcctg cgcnnnnnnn nnngttatca
    36001 gctttaccat acccgatggc tataaagccg tgggcataca aaacctcaat atgaatatag
    36061 ataacgaaac cggcaccttt gccgtacagg cgtcggtaga aggcaacaca cttagcatac
    36121 tggtaaaaaa actgtacaag caggttacgg taaaaaaaga agactggccc aaactattag
    36181 aaatgctcga tgccgctttc aacttttcgc agaagaagat tttgctgaag aaaataaact
    36241 aatcagaaag gcagcagttc caaaggcagc cggataatga gccgcttctt tatcaatcct
    36301 ttcttaagtt gacggtagct gatgcgtttg ccggaagagg ggaaacggta ggtaagctga
    36361 ccctggtaaa caatagtatt aagctttaca gcagcagcgc cttcgggcag gtaccatgca
    36421 tttttaatat cctcaatacc aatttgcagg ccgggattat aatattttat tgaaatggaa
    36481 aatcgcattg ttgcaatagc ctggcataaa tatacgctga ttatcaatag atatttatgc
    36541 ctggagtgtc gtatatttat gagtt
//
LOCUS       DAORLS010000004        35986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_602_length_35986_cov_2.268682, whole genome shotgun sequence.
ACCESSION   DAORLS010000004 DAORLS010000000
VERSION     DAORLS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 35986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35986
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_35986_cov_2.268682"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PK977_00700"
     CDS             <1..1261
                     /locus_tag="PK977_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF16644.1"
                     /translation="NKIIFTFDEFVDVQNVSENLLVSPLPQSLPGVDYKLKTVTVKLK
                     DSLEANTTYTINFGNSIKDFTEGNPLKEFTYTFSTGSYIDSLELNGKVILAETGKTDS
                     TLIVMLHTNGDDSAVINEKPRYIAKLDNKGNFTFKNLPAKTFYVYALKDEGGSRRYFG
                     EKQLFAFADKPVTIQSKNDPVVLYAFSSKPIAPAQPPPPATGLTLGRKNVAGTTDKRL
                     RFQTNLVNGQQDLLSDFILTFEAPLRLFDSAKVTLYTDSSFNPVKDYRFVKDSTGKKY
                     VLNQTWKENTLYHIILDKEFAEDSTGKKLLKNDTLSFKTKKVSDYGSLKIKFRGLDLS
                     KNPVLIFLMGGETIHKSFPLTSIDFSKTMFLPGEFEMRILYDNNKNGAWDPGQFFGKR
                     IQPEIVVPIERKISVKPAWQNEFEIAL"
     gene            1325..1954
                     /gene="recR"
                     /locus_tag="PK977_00705"
     CDS             1325..1954
                     /gene="recR"
                     /locus_tag="PK977_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRF16645.1"
                     /translation="MQFSSSLLENAVNEFAKLPGIGKKTALRLVLHLLKQDTKEVGHF
                     SEVIARMRNEIKFCQRCFNVADGDICSICANSIRKQETICVVESIRDVIAIESTQQYN
                     GIYHILGGIISPLDGIGPDQLNIESLISRVQKEKTEEIIFALNPNIQGDTTIYYIQKK
                     LSAVTVNPIRITTIARGIAFGGELEYADEMTLARSLQNRLPVESYVSGR"
     gene            2112..>3011
                     /locus_tag="PK977_00710"
     CDS             2112..>3011
                     /locus_tag="PK977_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRF16646.1"
                     /translation="MKTIQDCLKERILIIDGAMGTMIQRHKLTEADYRGVRFKNWHCD
                     VKGNNDLLNITQPEIIIGIHLQYLDAGADIIETNTFSSTSIAMADYEMQSLAYELNVA
                     AAQCAREAIKRSGKEAWVAGAIGPLNKTLSLSPDVNNPGFRAVTFDEVVNAYYEQVKG
                     LVDGGTDILLIETIFDTLNAKGAIFAIKKYFRDTGKTPLPIMISGTITDASGRTLSGQ
                     TLEAFFTSVMHARPLSIGLNCALGAKEMRPHIEELSQIASCFVSAYPNAGLPNAMGEY
                     DEKPEDTGHYLEEWAKEGFVNIVG"
     assembly_gap    3012..3021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3022..3119
                     /locus_tag="PK977_00715"
     CDS             <3022..3119
                     /locus_tag="PK977_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16647.1"
                     /translation="CGTTPDHIRHIAEHVKDIKPRQLPVVEKELA"
     gene            3215..>4172
                     /locus_tag="PK977_00720"
     CDS             3215..>4172
                     /locus_tag="PK977_00720"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRF16648.1"
                     /translation="MQESTVIRPYLRLAGLEPLVIRPETNFVNVGERTNVTGSKKFAR
                     LIKENKYEEALSVARQQVENGAQVLDVNMDDALLDGIQAMTTFLNLLQSEPDIAKIPI
                     MIDSSKFEIIEAGLKCVQGKCIVNSISMKEGEAKFIEQAIICQSYGAAVIVMAFDEKG
                     QADTKDRKIEICHRAYKILTEQVGYDPQDIIFDPNIFALATGMEEHNNYGVDFIEATR
                     EIKKLMPLTKVSGGVSNLSFSFRGNDHVREAMHSVFLYYAIHAGMDMGIVNAGQLVVY
                     DEIEPQLRELCEDVILNRNNDNNEATEKLIAFAETVKAKAKTE"
     assembly_gap    4173..4183
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4184..5907
                     /locus_tag="PK977_00725"
     CDS             <4184..5907
                     /locus_tag="PK977_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRF16649.1"
                     /translation="DEAWRNTSVEERLKHALVNGITDYIETDTEEARLKYPRPLDVIE
                     GPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKKSVAWLTPFIEEEKRNNPNTGNGNVP
                     KVLLATVKGDVHDIGKNIVGVVLGCNGYDIIDLGVMVPADKILDTAEKEKADIIGLSG
                     LITPSLDEMVHVAHEMKRRNMKQPLLIGGATTSRMHTAVKIAQQYDHGVLHVLDASRS
                     VTAVSTLLSKEKDEFLKKTAEEYQGLRDQFLNKNKKELIPYSEAVVTKEYFDWKNYKP
                     VKPVVDGVKVLKEFDLSIIARYIDWGPFFIAWEMPGRFPEVLSDKIFGTEATRLYNEA
                     QKMVQQIIEEKWFTADGIIGFWPASSNNADTITLSTEKGDVKLESLRQQLKKAVGQPT
                     YSLADFVKPKEIGEDFMGSFAVTIHGAKKHIDHFAAQHDDYNKILVQVLADRFVEAFA
                     ECLHEKVRKEYWGYAKDETLTNEQLIKEEYKGIRPAPGYPACPDHTEKLKLFSLLNVT
                     ENIGIELTESLAMNPPASVCGWYFAHPQSHYFGLGKISRDQLEDYAKRKGMSMEEAEK
                     WLRPVLE"
     gene            complement(5909..6397)
                     /locus_tag="PK977_00730"
     CDS             complement(5909..6397)
                     /locus_tag="PK977_00730"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF16650.1"
                     /translation="MNEIVQTEEQQKQGLFDDHIEYTQASQGQRFLNWLIDNLFMRFA
                     LSYATGYVVGYLLSYIAPDLLLSIAYGDRGANYYLLAIILGYFNYLLYYTFCEAAFKG
                     YTLGKLLTGTRAIRNDGGNITFKDAILRSLCRIVPFEVFSGLGDEPWHDAWTKTTVIK
                     TR"
     gene            complement(6406..7017)
                     /locus_tag="PK977_00735"
     CDS             complement(6406..7017)
                     /locus_tag="PK977_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRF16651.1"
                     /translation="MAVNTIIWDLGGVLIDWNPRYLFNESYFETEEKREYFFKHICSN
                     DWNEEQDAGRSIVEATQELVKKFPEWEKAIRDYYGRWTEMLKGPITESVDIFRRLKEL
                     GNYKMYALTNWQANLFDIALVRYDFLHWFDGRVVSGEEKTRKPFPEFYQRLLDRYQVD
                     PAKALFIDDNLRNVIAAEELGISSIHFKNSVLLKQDLSAMNLL"
     gene            complement(7020..7280)
                     /locus_tag="PK977_00740"
     CDS             complement(7020..7280)
                     /locus_tag="PK977_00740"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRF16652.1"
                     /translation="MSAADILGYAAGALTAFTFLPQVLKTWKEKSAKDVSLYMFIIAF
                     VNEIMWLVYGVMIDNWVIILTNAVMLVMSGIMIMLKMKYNHQ"
     gene            complement(7286..>7508)
                     /locus_tag="PK977_00745"
     CDS             complement(7286..>7508)
                     /locus_tag="PK977_00745"
                     /inference="COORDINATES: protein motif:HMM:NF025862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HRF16653.1"
                     /translation="YQAIIYRDADNADWKNNPEAYTIEKKLVNKNSVLGLKLAPGGGA
                     AISMIPLTAEQQLQMKKEIKALPKSKKKK"
     assembly_gap    7509..7660
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7661..>8020)
                     /locus_tag="PK977_00750"
     CDS             complement(<7661..>8020)
                     /locus_tag="PK977_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HRF16654.1"
                     /translation="RLTGLHRTYPNWIASEAARGNEFNAWSVGNPPEHETILPFTRLM
                     GGPMDYTPGIFKIKMSHYNPEKKEQVHTTLAKQLALYVTMYSPLQMAADLPENYEAKP
                     DALQFIKDVAADWDETKV"
     assembly_gap    8021..8054
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8055..>9422)
                     /locus_tag="PK977_00755"
     CDS             complement(<8055..>9422)
                     /locus_tag="PK977_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HRF16655.1"
                     /translation="STTVFADQSVTLHSPDGNVQLGLLHRSNGDLTYRVFYKGKTVVA
                     PSGLGMRLKSPELSLSKFDLLGIDSSVTDETWKPVWGEVSQIRNNYKQLLVKLADRSG
                     SGIQLEIIFKVYNDGVGFRYSFPQQAKLNHFIIGEELTQFAMSGDHKAFWIPGDYDTN
                     EYPYYTTNLSGVDATGGGAAQEIHAKTFFDKSAVQTPLMLKSADGLYINIHEAALVNY
                     PAMNLVVDKNTFTLNSHLVPDAVGNKAYLQAPAVTPWRTIVVSDKAGEVLTSKLILNL
                     NDPSKIEETSWIKPMKYVGVWWEMHVGRSSWDYSGGQVGSQQATKVPHGANTANVKRY
                     IDFAAKHGFSGVLVEGWNTGWEDWFGQWKEDVFDFVTPYPDFDVKELKQYAANKGVKI
                     IMHHETSGSVTNYERWMDTAYRFMKKFGYDAVKTGYVGRIIPRGEHHDGQWMIKHYER
                     GAQKTA"
     assembly_gap    9423..9441
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9532..10275)
                     /locus_tag="PK977_00760"
     CDS             complement(9532..10275)
                     /locus_tag="PK977_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRF16656.1"
                     /translation="MEASAKSKRRIIPPKAHVFLEGPKSRGYELSFAFRVFWQFLKGF
                     RNLHFIGPCVTVFGSARFKEDHIYYQQAREIGKRIAELGMTTMTGGGPGIMEAANRGA
                     FENGGKSVGCNIRLPFEQKANPYMQKWVTFEHFFVRKVLLVKYSYAFIIMPGGFGTMD
                     ELFETLTLVQTQTITRFPIVLFGKEYYQPMMEMVDAMIEEGTVSRDDINLVYLTDSVD
                     EAINYIHNYIRENYQVKPRRRRWWLFEKR"
     gene            10372..>10948
                     /locus_tag="PK977_00765"
     CDS             10372..>10948
                     /locus_tag="PK977_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRF16657.1"
                     /translation="MDWLKTDPADVICVQETKAVQGDVDHKPFHELGFHNYWFSAQKK
                     GYSGVAVFTKIKPDNVEYGNGHGPSDDEGRVIQLDFGKTRLINAYFPSGTSGDERQVF
                     KYQWLDEFNSWLKKLRKKHPNLILCGDYNIAHKEIDIHDPKGNKNSSGFLPEERAWMT
                     NFLEDGWVDTFREFHPEPHRYSWWSQRFPTVR"
     assembly_gap    10949..10972
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11090..11395
                     /locus_tag="PK977_00770"
     CDS             11090..11395
                     /locus_tag="PK977_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRF16658.1"
                     /translation="MIIYNVTVKVDHSIAEPWLAWLKEEHIPDVINTGCFTHASILRL
                     LEVDETDGITYAVQYHSASKELYNRYISDFAETMRNKGFDKWGNKFIAFRSVMMVVH"
     gene            11683..11952
                     /locus_tag="PK977_00775"
     CDS             11683..11952
                     /locus_tag="PK977_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRF16659.1"
                     /translation="MNKAELIAKVSDDAGITKTQANAALDSFIEAVTKTLKGGGKVTL
                     VGFGTFSVSKRAARNGRNPQTGEVIKIKARKVARFKAGKELAAKL"
     gene            12048..12161
                     /locus_tag="PK977_00780"
     CDS             12048..12161
                     /locus_tag="PK977_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HRF16660.1"
                     /translation="MGRGDKKTAKGKRFKGSFGKSRPAKKAAVKKKAAKKA"
     gene            complement(12225..13247)
                     /locus_tag="PK977_00785"
     CDS             complement(12225..13247)
                     /locus_tag="PK977_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16661.1"
                     /translation="MDGQLSLSIIEIIVLMLGAIILGITIHFFITSRKSLKDSMADAA
                     GSKLSRELDDWKRRFFNEVELKDKQIENLQKLVADAEENSNIFSIEAEEVRKQNKKLQ
                     QEIELLKNQVPQSSGEKPGYMEQLKLAQNSLLEHNEKINQLLEQIDIVKETEAKQQEV
                     LKYNEELNEEIELLKHKLAQKENEINAIRQKENLTSEMNSMLDSAYSEFGVLQEKMQK
                     LEAQVAVSKKINLEYEDLKEGYYRISQDYEEQKHKYNHLVSENRQLQADLAETEDKLK
                     EANFQRQQLQKRVTYLEELNSDMQVVADANKRLEGQLKRIGELESMLNVLAEERDQLA
                     KKQYNA"
     gene            complement(13384..14097)
                     /gene="pyrH"
                     /locus_tag="PK977_00790"
     CDS             complement(13384..14097)
                     /gene="pyrH"
                     /locus_tag="PK977_00790"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRF16662.1"
                     /translation="MLPKYKRILLKLSGEALMGDKSFGFDTDVIAQYAQDIKSITELG
                     VQVAVVIGGGNIYRGMNEAETGIERAHGDYMGMLATVINGMAVQAGLEKVGVYTRLQS
                     AIKMEQIAEPYIRRRAIRHLEKGRVVIFGAGTGNPYFTTDTAGSLRAIEINADVILKG
                     TRVDGIYTADPEKDPSATKFNVITFQECLTNNLRVMDMTAFTLCMENNLPIIVFDMNK
                     EGNLRRVVTGEKVGTLVRG"
     gene            complement(14135..14935)
                     /locus_tag="PK977_00795"
     CDS             complement(14135..14935)
                     /locus_tag="PK977_00795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF16663.1"
                     /translation="MSNFDQYFNANKELWNQRTIVHRDSEFYNLAGFKKGETVLTPIE
                     LAEVGDVNGKKLLHLQCHFGMDSLDWARRGAHVTGVDLSDIAIKEARSLNMELGLSAE
                     FVCCNVLDTADYIKQEFDIVFTSYGTIGWLPDLKPWANMISRMLKPGGFFYIAEFHPV
                     IWMFDDDFTHIQYAYENREVIVTESKETYTDRNADIRGKEYSWNHSISEVLNALIGAG
                     LTIASFNEFMYSPYPCFQQVVEIEMGKWHIKGMEGKLPMVYTIRAVKQ"
     gene            complement(15000..15707)
                     /locus_tag="PK977_00800"
     CDS             complement(15000..15707)
                     /locus_tag="PK977_00800"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRF16664.1"
                     /translation="MKVISKVFFGLVAGLFLFTACSKEPSNPIERSYSNIDFKKIDAG
                     DQHRLTITKGTGFSIIARGEERDVNDLVIRVIDSSLKIEYNRYKSNRKRVYFTITLPS
                     LEGLVLSGQSEATVSGFSETDLVNLVVSGQSACWINMNAPKFKLLASGQSKIEFQGGV
                     ATGFEGEASGQSEILAYGLSPVVFAEVTATGQSTIKVRVGNSLKANASGQSRIYYRGD
                     PLITQIVSTGDSRIIKE"
     gene            complement(15747..16427)
                     /locus_tag="PK977_00805"
     CDS             complement(15747..16427)
                     /locus_tag="PK977_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF16665.1"
                     /translation="MKKGIVLCLALAGVMVSHAQIRVGLYGSASLSKSSFTKEEGVVT
                     TTKPVYAPAAGLILDIPLSSEFSIRPSLGFIRKGIKAKATASEMGIISSIKSDVKMNY
                     AELPVLFTYKKQLGSTTFLIGAGPSAGVGLGGKVKTESFIEGIPEPIFSYEVNPFKKS
                     DHIEQPFKRFEFSGAGMLGVELKNGLFFSANYLHGFSNISGDAPDYKFRNRTVSVSVG
                     YFFGAKKG"
     gene            complement(16653..16838)
                     /locus_tag="PK977_00810"
     CDS             complement(16653..16838)
                     /locus_tag="PK977_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16666.1"
                     /translation="MKGKYITRNIQLACLATEKPKQETDPKMVIDADCVEVSNKCEGD
                     NDTVVNTWLVFGGSDAS"
     gene            complement(16988..>17215)
                     /locus_tag="PK977_00815"
     CDS             complement(16988..>17215)
                     /locus_tag="PK977_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16667.1"
                     /translation="IFSKLKPYLFTNNREHALALADRGQKPFKEGPGAIGYFVGFRIC
                     EAYVKKHGKESWKDIYSLSPREVLLKSGYGE"
     assembly_gap    17216..17251
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17252..17970)
                     /locus_tag="PK977_00820"
     CDS             complement(<17252..17970)
                     /locus_tag="PK977_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16668.1"
                     /translation="MRKLLIVLLMSLGLLHAYAEGEIDGETYYRVTKVKDSVNLFNGN
                     LRLINIYKQQIAVLYETRNLTEQQVRAELIKRTYLPYQEFWEGYVGDSTVYYEQVMKP
                     LIKDSIRIMAKKALTFSSFNIDNFFNLTASVLNCYSGRTPSGTWFIAFGSGVTDMGGF
                     GNGRMVLDLTHSKAEIGYVQMILPHEINHQIYENTILPDTTARGLYRVINEGFAVYMN
                     QVVFGDKYELHEYLQYSVSGL"
     gene            complement(17993..18466)
                     /locus_tag="PK977_00825"
     CDS             complement(17993..18466)
                     /locus_tag="PK977_00825"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRF16669.1"
                     /translation="MKLNFWTISIVTIFIMITGITYGKDLPPVMRIKLEQHFTYLEVS
                     DHISVVLTNETGKDISVDGDRSKFGKVKALVKKGKLSIWLQGSNMGDKLTVYVPARLL
                     KQFVINGDSKVVTEEVLDNRKLDVVVNGACQLSLRSKGKINVTGTNEFEFQHSYE"
     assembly_gap    18533..18733
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18734..19472
                     /locus_tag="PK977_00830"
     CDS             <18734..19472
                     /locus_tag="PK977_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16670.1"
                     /translation="SAKIALDNNEWGKAEEFAVKGRQGIMINQKLSFGSYRTVSVKRS
                     WTKGSSYFAGWASGRPGYDDYERVIGTEYSQRKQTIRFEMTDDKGNESSVFCLSRTRE
                     KDFMIGNNPNSLINILLDISGKGDISENTFWIKIFINNQSRSWEMLLDNEAAQRNSKS
                     YTGVVAQSRDNYYTIHPIFKMTGKNQKVYTMPVGSVGFELRNKEGKPVAAVSAIDKGS
                     VYLNSTDPGEKFLLSNICAAILLRESI"
     gene            19563..>20043
                     /locus_tag="PK977_00835"
     CDS             19563..>20043
                     /locus_tag="PK977_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF16671.1"
                     /translation="MRRLMLLAVLLNGLYAHTQQGYTFNRISTDDALGLSSNAVYCTY
                     QDKKGYIWVGTANGLQRFDGSKFVSFGNSDPGSTLLPISDLVQIIPDDNGMLWLFFGS
                     RLEVGLFDPISFRYHTVPIKTSKPIPARSGMKLWKDSQQNLYLVIWKFGILRYNKNKH
                     "
     assembly_gap    20044..20170
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20171..>21134
                     /locus_tag="PK977_00840"
     CDS             <20171..>21134
                     /locus_tag="PK977_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF16672.1"
                     /translation="DSGIAIYDILSKKIYTSSNNPLKIPLLDNKNIIPGTTEIFIDSR
                     RRHWIFNWTSGQHKWCFDEKGTLLKDTAGLNDNREYSELTSFFETKEKVLWVYGANGL
                     FNYDSDVNRFFFYKPGVTSETGIPYQSAHYIMEDRDGSIWISTDNGLFFATPESGTYG
                     VVNILFDERNGGIEITDLLQLKSGQYWLSTWGKGIITLDKTFAKYSADVYKLMPKMDQ
                     VSFAQYYQTWALYQHTDGKIWIGCQAGKYIIYDTLTQKSSFAELKEADGATIRFITGD
                     KNGNIWFSTQRGHIINYDGKKFTVVQQFGTIIPKILIDNNGLLWL"
     assembly_gap    21135..21243
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21244..>21517
                     /locus_tag="PK977_00845"
     CDS             <21244..>21517
                     /locus_tag="PK977_00845"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF16673.1"
                     /translation="DIDQINDTTIAYGAGALNLINTRTGKVTWRTFDDGLPGNSIRRI
                     RTDRNGNLWIITKNGLSKYNPRTNRFTPYGRKDGITLANFTTAADF"
     assembly_gap    21518..21529
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21530..22632
                     /locus_tag="PK977_00850"
     CDS             <21530..22632
                     /locus_tag="PK977_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF16674.1"
                     /translation="ILFAGSNGLMYFMPSLFENKKPPANVVITDFKLFNDYLPLDSLL
                     KRPKLEFNSDENSFSIYFSSLSYSQKEKLTYYYKMEGVDKEWIKADRQNYVNYPLLPP
                     GHYTFSVYCENIDGIRSKEITSFEIYIKPPFWKTYWFISTLLLIILLFIYALHRLRVN
                     KLLAVEKIRNRVARDLHDDMGSTLSTINILSTMAKSKLNSDNVKTSEYISKISENSQR
                     MMEAMDDIVWSIKPMNDSMQKIIARMREYATNILEAKDIEVSFNIEEEVNDVKLDMEA
                     RRDFFLVFKEAVNNAAKYSKAEKVKINLFILTGQLTLEVIDDGEGFDVALADSGNGLS
                     NMQKRADAMQGRLQIISKKGNGTIVKLAVPFN"
     gene            22642..23748
                     /locus_tag="PK977_00855"
     CDS             22642..23748
                     /locus_tag="PK977_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRF16675.1"
                     /translation="MNKTLSQKFRFFTFICIALLAYVHGYNLNNTYLAPYSTVEEPLT
                     VTSFFEYFIANGLLRFRIPMLFIISGYLYATYDHRPYLQQIQKRFKTLIIPFFLWSAA
                     GLALTFLLQQFTYTADLVHTAGIDQLGDNRPYTEIGWEGILRRWLLHPVSYQLWFIVS
                     LFIYNLIYPVIRWLVVKIPFIWLPLTFLLFFSLFQFIYVDGRGIFFFSLGIWLQKKKI
                     SIDKQPEWFSAGLAFILFIGLTVIKTFMAFELEPNQFSTSLTLVIFYQLSVITGILAM
                     WFGLDKVVRWALRKKWFYKASGFSFFLYGAHVPLLPYAMTMALTELSFLPYYRLFCYI
                     VIPGLIVVLCIWIGRFVRKFLPGIYFTLTGSRGF"
     gene            23846..24478
                     /locus_tag="PK977_00860"
     CDS             23846..24478
                     /locus_tag="PK977_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF16676.1"
                     /translation="MIKIMLYEDNPQLREGLTMLIDGSEGFQVLASFKNCSNVVQEVE
                     AFKPDVILMDIDMPVVNGIEGLKLIRQHNHDVKVLMLTVFDDNKNVFEALKNGANGYV
                     LKKTPPAKLLEYITEAASGGAPMTSSIATQVLKMFSEVQAPPGEDYNLSDREKEVLQH
                     LVNGYSYKMIASEMFIAIDTVRSHIKKIYEKLHVNSKSEAVAKAFKDKIV"
     gene            24639..25232
                     /locus_tag="PK977_00865"
     CDS             24639..25232
                     /locus_tag="PK977_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF16677.1"
                     /translation="MRTMILLLSVYIAGIAIVTTACKNSSTGNPPVSEKKNDSSEIVK
                     RGEYLVTIGVCDDCHSPKTMGARGPEIDMERRLSGFPGTRPAPSYNKELMQSGIVQFN
                     EDFTSAAGVWGISFAANLTSDATGIGNWPLENFKYAIRHGKLKGMETGRDLLPPMPWT
                     NFSKMTDEDIEAVYTYLKTIKPVNNVVPAPIPPAGGK"
     gene            complement(25295..26131)
                     /gene="tsf"
                     /locus_tag="PK977_00870"
     CDS             complement(25295..26131)
                     /gene="tsf"
                     /locus_tag="PK977_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRF16678.1"
                     /translation="MSTVTISAADINKLRQATGAGMMDCRKALTETNGDFEAAIDWLR
                     KQGQKVAAKRSDREAKEGVVIAKTTADNKTGIVVCVSCETDFVSKNADFVAFGQSIAD
                     AAIANNVANLDQLMEVKIGDLTVKALVDEKLATIGEKIGITKFERIDAPFVASYIHGA
                     YRIGVLVGMDKEAAEAGKDVAMQIAALNPVAVDAASVPAEVIAREKDIIMELMKQDPK
                     MAGKPDEMLAKIAEGKMGAFFKEQTLTAQAFVKDGSKTVEQYLKDSGDVKVTSFKRVA
                     LG"
     gene            complement(26261..26653)
                     /gene="rpsB"
                     /locus_tag="PK977_00875"
     CDS             complement(26261..26653)
                     /gene="rpsB"
                     /locus_tag="PK977_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRF16679.1"
                     /translation="MFIVDIGHEHIALAEAKRLGVSTFGVVDTNCDPNKVDFAIPAND
                     DATKSIAIIVNYITAAIAEGLAERQAAKDEEVEEMTDDEKERKAAKLLAEAEAEGGRG
                     GRGRGAGGGGQRRRTPAGGSRGPGGARR"
     gene            complement(26628..27125)
                     /gene="rpsB"
                     /locus_tag="PK977_00880"
     CDS             complement(26628..27125)
                     /gene="rpsB"
                     /locus_tag="PK977_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRF16680.1"
                     /translation="MENNTSLQQQLLEAGVHFGHLKKKWNPKMLPYIFAEKKGIHIID
                     LNKTVECLQETAAALKQIARSGKKILFVGTKKQAKDIVSECAKKVNMPFVTERWLGGM
                     LTNFNTVRKSVKKMQSIEKMLGDGSFDSITKKERLTLSRDKDKMEKVLGGIAQLGCVH
                     CRYRA"
     gene            complement(27165..>27423)
                     /gene="rpsI"
                     /locus_tag="PK977_00885"
     CDS             complement(27165..>27423)
                     /gene="rpsI"
                     /locus_tag="PK977_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRF16681.1"
                     /translation="NQVERPLKTADVMNKFDVKINAAGGGLKGQAEAAMLGISRVLVE
                     LNPEFRPALKAAGLLKRDPRSVERKKFGHKKARRSYQFSKR"
     assembly_gap    27424..27557
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27603..28046)
                     /gene="rplM"
                     /locus_tag="PK977_00890"
     CDS             complement(27603..28046)
                     /gene="rplM"
                     /locus_tag="PK977_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRF16682.1"
                     /translation="MSKLHFTTKHANEATVKRNWYVVDGTNQTVGRMCAKIAAMLRGK
                     HKASYTPHVDTGDYIVVINCEKVVFTGNKLEQKIYDHFTGYPGGRKEETAANLQKRRP
                     EVVIERAVKGMLPKNRLGRKMYKKLFVYAGAEHPHTAQQPKELKF"
     gene            complement(28261..28977)
                     /locus_tag="PK977_00895"
     CDS             complement(28261..28977)
                     /locus_tag="PK977_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16683.1"
                     /translation="MEEKILKGTISFVNYEKNFATIEYLQGMKKKSVNCKTGFESTGR
                     KSHHFRMGDTVSFQLRLSDRGDKMTACNIKYLYNTELDLLIQRAAIENRFSGYLKKTG
                     DKYFVKEWNSYIFFPLQLSPWEKPPVETAENEAISFCLINLDRPNSIVAELFSHNYIP
                     AYKKAVQHFKNEIAIDAVVYKVSPHGIYLNLFDDKIQAKLPVDTAETGSIKEGDIIPV
                     LISHLSNTRIVIKRLIGLAS"
     gene            29056..29658
                     /locus_tag="PK977_00900"
     CDS             29056..29658
                     /locus_tag="PK977_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRF16684.1"
                     /translation="MSNRNLGRSVFGDPKNAGNLMPLEEAHTLLEEWVPNEKLRLHMK
                     QVAANMKAWAIEKEGADELTAQKWELAGLLHDADWEKYPDDHCRIIIEELERRNIDPD
                     VIHCIASHGPSVFGVEPVSKMDKMIYAIDELSGFIHAAALIRPTLYEGLEVKSVLKRL
                     KTPSFAAQVSREEISDALSKTDTTLEELIQFIIDHQKSVV"
     gene            complement(29907..30104)
                     /locus_tag="PK977_00905"
     CDS             complement(29907..30104)
                     /locus_tag="PK977_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16685.1"
                     /translation="MLDIRIPIGLLFTVLGLLVTAYGLITNADTELYKKSFQYNVNLW
                     SGLLMLVFGVIMLLLARKKKT"
     gene            complement(30123..>31483)
                     /locus_tag="PK977_00910"
     CDS             complement(30123..>31483)
                     /locus_tag="PK977_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223414.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRF16686.1"
                     /translation="RGLNAILFAVMTVLASGISMYALALLMEKVLGWDFNLSLWLSAA
                     IVLAYTYLGGLSSAIYNEVLQFFIIVIGLLPIVVLSLHEVGGWSGLKESLAPIVTEKG
                     FAGDTFTTTWKYTGSANGNPMGVEWYGILFGLGFVLSFGYWCTNFLIVQRAMAAESTT
                     AARNTPLIGAIPKMIIPFLIIVPGIVALVLMNKADSGFTIMENGKANYDLTIIKLLEY
                     YLPTGVLGLGITALLASFMSGMAGNVSAFNTVWTYDIYQSYIRPATNDKEADAKHYLK
                     VGRMATVFGVLVSIAAAYLAGLFGNIMDFLQTIFSMINAPLFAVIFLGMFWKRATGHG
                     AFIGLLGGFFVALLHHGLTVPAGATTLVKGGWLGVIHTYPVEMAQSFWTAIFACASAA
                     IIAVGISLSTAQRKTDDELRGLVYSLTPKIKEDHVAWHKRTNTLAIIVLTMMVILSIL
                     FW"
     assembly_gap    31484..31495
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31496..31847)
                     /locus_tag="PK977_00915"
     CDS             complement(<31496..31847)
                     /locus_tag="PK977_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HRF16687.1"
                     /translation="MGNSIFHLQWIDYAIMIIYFLFVLGIGWVLKRYMKDANAFLEAG
                     RSMPAWVAGLAFISTNLGALEVMGMTGSGAKYGMLTTHFYWIGAIPAMVFLAIFMMPF
                     YYGSKARSVPEYLKL"
     gene            complement(31873..33024)
                     /gene="galK"
                     /locus_tag="PK977_00920"
     CDS             complement(31873..33024)
                     /gene="galK"
                     /locus_tag="PK977_00920"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRF16688.1"
                     /translation="MRDYKIPDQLKQFITADTIVVRSPGRINLIGEHTDYNEGFVLPA
                     AIDKAVYVTVEKNPGTRLNLQSVDFNESIEVEIRDIKPVPGHWSTYIFGVVDQFIRKG
                     QFVTGFKLSIYGDVPLGAGLSSSAAVECAVAFALNELLQSGFSRKELALMAQAAEHQY
                     AGVKCGIMDQFASLFGKKDHVIRLDCRSLEYEYFPLELGEYVIVLFDTMVKHSLGSSE
                     YNIRRSQCEEGVMLIGQNYPSVHSLRDATMEMVNECIGEFETAHRRCKYVVEEIDRLQ
                     KGCEDLQAGDLIAFGKKMFATHDGLSKEYEVSCKELDYLVDNVRNNPAVIGARMMGGG
                     FGGCTINIISKDMVEELVAKLSEDYKKDMGKELKHYLVNIEDGTSVIGR"
     gene            complement(33035..>35986)
                     /locus_tag="PK977_00925"
     CDS             complement(33035..>35986)
                     /locus_tag="PK977_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRF16689.1"
                     /translation="NKSGYVGAAYDVTDYINFYGDNVLVVRVDATHYEGWFYEGAGIY
                     RHVWLNKYSNVHIAKDGIFAWSAIAGNKATVTIESLVQNNSTTNTVTTVSAYITDRDG
                     KFVARSKDISLSLAVNKAGTSINTVSVTGFRKWTLDDPYLYRVVVELRSDNRLTDRKQ
                     IRFGLRQIEVKSNGVFLNGQHVKLKGVNNHQDHAGVGSALPDYLQYYRISLLKRMGVN
                     AYRTSHHAPTPELLDACDSLGMLVMDEQRLLNSSPEYMDQFERLIRRDRSRTSVFMWS
                     IGNEEGWIHTNSFGKRIAQTFIAKQKELDPTRTCTYAADLPNVYNGINEVIPVRSFNY
                     RQFAVADYHRDHPEQPIIGTEMGSTVTTRGIYEKDTIRGYVPDQDITAPWWASRAEDW
                     WTLAANNDYWLGGFIWTGMDYRGEPTPYQWPNINSHFGVMDMCGFPKNIYYYYKSWWT
                     DKDVLHMSPHWNFREKGPGWNKPKGDLIDVWVNTNADNVELFLNGKSLGKKDMPRNSH
                     LKWSVNYEPGKLEAVAYKKGKRLYKKIETTGLPFEVVVTPYKTTILADGKDATVLNIT
                     VLDREGREVPDADNLIKFSIEGPGKIIGVGNGDPSSHEPDKSTNGIWQRTLFNGKCQV
                     IIQAGTETGMIKFEAKATGLWPGGTDIQTISPGSVNKISFDSKYKLTPEQSKPVEVGK
                     MLGADISFLPELEARGIKFSDNGVEKDAIEILKNHGFNYVRLRLFNDPARDSGYSPGK
                     GFCDLENTKKMAKRVKAAGMKLLLDFHYSDYWADPGKQYKPAAWRNLSFEELKKAVYN
                     YTKEVMQQLKEQGTAPDMVQVGNEINHGIIWPEGNVSNFDGLAQLINAGTKAVKDIDP
                     SVIMMLHVALGGQNDESVFFIDNMMARGVHFDVIGESYYPKWHGTLEDLETNLEDLSR
                     RYNKDVIVVEYSHRKEEVNKLAFEVPGGRGKGTCIWEPLSTWEKFFDDKGKSNDLLKL
                     YDEISNKYLKD"
BASE COUNT        10484 a         7628 c         6704 g        10289 t
ORIGIN      
        1 caataagatt atattcactt ttgatgaatt tgtagatgtc cagaacgttt cagaaaacct
       61 gttggtatcc ccattacccc aaagcctgcc gggtgtagat tataagctga aaacagtaac
      121 tgtaaaacta aaagactctc tggaggcaaa tactacctat accatcaatt ttggcaactc
      181 aattaaagac tttacagaag gaaatccgtt gaaagagttt acctatacat tttctaccgg
      241 cagttatatt gactcactgg agcttaacgg caaagtaata ctggcagaaa ccggaaagac
      301 tgattctaca ctaattgtta tgcttcatac caatggggat gactctgccg taataaacga
      361 gaagccgcgg tatatcgcta aactggataa caaaggaaat ttcactttta aaaacctgcc
      421 tgctaaaact ttttatgtgt atgccttaaa ggatgaaggt ggaagccggc gttattttgg
      481 agaaaagcaa ttattcgcat ttgctgataa acctgttact atccaaagta aaaatgaccc
      541 tgtagtatta tatgcttttt catcgaaacc tattgcacca gcacaaccgc cgccaccagc
      601 aacgggacta acccttggca gaaagaatgt ggccggaaca accgacaaaa ggttaagatt
      661 tcaaacaaac ctggtaaacg gtcagcaaga ccttttatcc gatttcatcc tgacatttga
      721 agcaccactt cgcttatttg actctgcaaa agtcactctt tatacagaca gctccttcaa
      781 cccggtaaag gattaccgtt ttgtgaaaga cagtaccggc aaaaaatatg tgctcaacca
      841 gacctggaaa gagaatacat tgtaccatat tatcctggat aaagaatttg cagaagactc
      901 tacaggcaaa aagttactga agaacgatac cctgagcttt aaaacaaaga aagtttccga
      961 ctatggatca cttaagataa aattcagagg tctggactta tcgaagaacc cggtattaat
     1021 atttctgatg ggaggcgaga ctattcataa atctttccct ctgaccagca ttgatttttc
     1081 gaaaacaatg ttcttgccgg gtgaattcga aatgcgcata ctttacgaca ataataaaaa
     1141 tggggcctgg gaccccggtc agttttttgg aaagcggata cagccggaaa ttgttgtgcc
     1201 aattgaacga aagatttcag taaaaccagc ctggcaaaac gagtttgaaa tagcactata
     1261 aaaggtcatt aaaaacatag cccattcact actcactatt gcccattcac tatctttgtc
     1321 ctccatgcaa ttctcttctt ctttattgga aaatgcagta aatgaatttg ccaaactacc
     1381 gggcattggt aaaaaaactg ccctgcggct ggttttacat ttattgaagc aggacaccaa
     1441 agaagtcggg cattttagcg aagtaattgc ccggatgcga aatgaaatta aattctgcca
     1501 gcgttgtttt aatgtggcag atggcgatat ctgctctatc tgtgctaatt cgattagaaa
     1561 acaagaaact atctgtgtgg tagaaagcat ccgtgatgta attgctatcg aaagcaccca
     1621 gcaatacaac ggcatttatc acatcctggg aggaatcatt tcgccattgg acggcatcgg
     1681 acccgaccag cttaacattg aatcattgat cagccgggta cagaaagaaa aaacagaaga
     1741 gattattttt gcgttgaacc ccaacataca gggcgatacc actatttatt acattcagaa
     1801 aaagctctct gcagtcaccg taaatcccat ccgtattact actattgccc gtggcattgc
     1861 ttttggtgga gaactggagt atgccgatga aatgacactg gccaggagcc tgcagaacag
     1921 actgccggtt gaaagctatg tatctggccg ctaaggctta tcctgcttgc tttttatacg
     1981 gataaagctt agttttgctg ccaattcagg cggatttgtt tattgttcgg cctgctccaa
     2041 acagaactaa taaacggtac gaaactccct ccatagtttc ccaacgttta tagttcgcaa
     2101 acaattgaag tatgaagaca atccaggatt gtttgaaaga acgcatattg atcattgatg
     2161 gcgccatggg caccatgata cagcggcata aattgaccga agccgattac cggggtgtac
     2221 gatttaaaaa ctggcattgc gatgtaaaag gaaacaatga cctgctgaat attacacaac
     2281 ccgaaattat cattggcatt cacctgcaat acctggatgc cggtgctgat attattgaaa
     2341 ccaatacttt cagtagtaca tctatagcca tggctgatta tgaaatgcag tcgctggcct
     2401 acgaattaaa tgttgcggct gctcaatgtg ccagggaagc aatcaaaaga tcaggcaaag
     2461 aggcatgggt ggccggtgct atcggacctt tgaacaaaac cttatcactt tcaccagacg
     2521 taaacaaccc tggttttcgg gcagtcacct ttgatgaagt agtaaatgca tactatgaac
     2581 aggtaaaagg gctggtagat ggtggtactg atattctact aattgaaacc attttcgata
     2641 ccttaaatgc caaaggtgcc atttttgcca taaaaaaata tttcagagat accggtaaaa
     2701 cgccgttacc cataatgatc agcggtacca ttaccgatgc ctccggcaga acactcagcg
     2761 gacaaacatt agaagctttt ttcacttctg taatgcatgc aagaccctta agcattggcc
     2821 tgaactgtgc tttgggcgct aaagaaatgc ggcctcatat tgaagagtta tcacaaatag
     2881 ccagttgctt tgtatctgct taccccaatg ccgggctccc caatgcgatg ggtgaatatg
     2941 acgagaaacc ggaagacacc ggccactatc ttgaagaatg ggcgaaagaa ggttttgtaa
     3001 atattgtggg cnnnnnnnnn ngttgcggca ccacaccaga tcatatacgg catatagctg
     3061 aacatgtaaa agatatcaaa ccaagacaac tgcccgttgt tgaaaaagaa ttagcatgac
     3121 gacaccacag tttttgatac tgttatcact tcttattctg gttgtaaata acttttcaat
     3181 gatcaggaag ttatttgtaa aaaagagctc atagatgcaa gaatccaccg ttatcagacc
     3241 ttacttacga ttagccggat tagaaccatt ggtcatcagg cctgaaacca attttgtaaa
     3301 cgtaggagaa agaaccaatg tgacaggctc caagaagttt gcccggctta taaaagaaaa
     3361 taaatacgaa gaagccctga gtgtggcccg ccagcaggta gaaaatgggg cacaggtact
     3421 ggatgtgaat atggacgatg ctttactgga tggcatccag gcaatgacca cctttcttaa
     3481 tcttttacaa agtgaaccgg atattgcgaa aattccaatc atgatcgata gctccaagtt
     3541 tgagatcatt gaagccggac tcaaatgtgt gcagggaaaa tgtatcgtga attccatttc
     3601 gatgaaagaa ggcgaagcta aattcatcga acaggcaatt atatgccaga gctatggcgc
     3661 tgcagttatc gtaatggcat ttgatgaaaa aggacaagcc gataccaaag acagaaaaat
     3721 tgagatttgt caccgtgctt ataaaatcct gactgaacaa gtaggatacg acccacagga
     3781 tatcattttc gaccctaata ttttcgccct tgccactggc atggaagaac ataataatta
     3841 tggcgttgat ttcattgaag ctactagaga aatcaaaaag ctaatgcccc ttaccaaagt
     3901 aagtggtggc gtaagcaacc tgtctttttc tttcagagga aacgatcatg tgagagaggc
     3961 catgcattcg gtttttttgt actatgctat tcatgccggt atggatatgg gtatcgttaa
     4021 tgcagggcaa ttggtagtat atgatgaaat tgagccacaa ttaagagaac tgtgcgaaga
     4081 tgtaatactc aatcgaaaca atgacaataa cgaggccaca gaaaagttga ttgcattcgc
     4141 tgaaaccgta aaggcaaagg ctaaaacaga ggnnnnnnnn nnnaagacga agcatggcgc
     4201 aatacttccg tggaagaacg gttaaaacat gccctggtca acggaataac tgattatatt
     4261 gaaacagata cagaggaggc aagactaaaa tacccacggc cattagatgt aattgaaggc
     4321 ccgttgatgg ccggaatgaa tgtggtaggt gatcttttcg gtgccggaaa aatgttttta
     4381 cctcaggtag taaaaagtgc aagggtaatg aagaagagtg tggcatggct aacaccattt
     4441 atagaagaag aaaaaaggaa taatccaaat accggaaacg ggaatgtacc aaaagtgtta
     4501 ttagccacag tgaagggtga tgtacatgat attggtaaaa acattgtagg agtagtgctg
     4561 ggctgtaatg gctacgatat tattgacctt ggcgtaatgg ttccggctga taaaatactg
     4621 gatacagcag aaaaagaaaa ggcagatatc attggactaa gcggcctcat cactccgtca
     4681 ctggacgaga tggtccatgt agcacatgaa atgaagcgcc gtaacatgaa acaacctttg
     4741 ctcatcggcg gagctactac ttcccggatg catacggctg taaagattgc ccagcaatac
     4801 gaccatggag ttttgcatgt gctggatgct tcccgaagtg taacagccgt gagtaccctg
     4861 ttaagtaaag aaaaagatga gtttctcaaa aaaacagcag aagagtacca gggcctgcgg
     4921 gaccaatttt taaacaagaa taaaaaggag cttattccct acagtgaagc agttgtaacc
     4981 aaagaatatt ttgactggaa gaattataaa cccgtaaagc ctgtagtcga cggagtaaaa
     5041 gtgttgaaag agtttgacct ctccattatt gcccgctata ttgactgggg accctttttc
     5101 attgcctggg aaatgccggg tcgcttcccc gaagtacttt cggacaagat atttggcact
     5161 gaagcaaccc gcctttacaa cgaggcacaa aaaatggtgc aacagattat cgaagaaaaa
     5221 tggtttactg cggatggcat aatcggcttt tggccagcca gcagtaataa tgcagatacc
     5281 atcaccctat caactgaaaa gggagatgta aaactggaat cattacgaca gcaattgaaa
     5341 aaagcagtag gccaacccac ttactcacta gctgacttcg ttaaaccaaa agaaattggc
     5401 gaggatttta tgggatcctt tgctgtaact attcatgggg caaaaaaaca tatcgaccat
     5461 tttgctgccc agcatgacga ttataataag atacttgtac aggtactggc cgaccgtttc
     5521 gtagaagcct ttgctgaatg cctgcatgaa aaagtgagaa aagagtactg gggttatgcc
     5581 aaagatgaaa cccttaccaa tgaacaactt atcaaagaag aatacaaagg aatccgtcct
     5641 gcacctggtt atccggcctg cccggatcat acagaaaaac tgaaactgtt ttctttgcta
     5701 aatgtgaccg aaaatatcgg cattgagtta actgaaagtc tggccatgaa cccaccagcc
     5761 tctgtatgcg gctggtattt tgcgcatccg caaagccatt attttggttt aggcaaaatc
     5821 agccgtgacc agctggagga ttatgccaaa agaaagggca tgagtatgga agaagctgag
     5881 aaatggttaa gaccggtact ggaatagctt atcttgtttt gattacagta gtttttgtcc
     5941 aggcatcatg ccagggctca tcgcctaaac cactgaatac ttcaaaagga acgatacgac
     6001 aaaggctccg taggatcgca tccttgaagg taatattacc tccatcattt cgtatagctc
     6061 ttgtacctgt cagcagttta ccaagagtat aacctttgaa agcagcctca cagaatgtgt
     6121 agtagagcag gtaattaaaa tagcccaata taatagctaa taggtaatag tttgcgcccc
     6181 ggtctccata agcaatagac aacaacaaat ctggtgctat gtaagaaagt aaatatccta
     6241 caacatagcc agttgcatag cttagagcaa accgcataaa gaggttatca attaaccagt
     6301 ttagaaaacg ttgcccttgt gaagcctggg tatattcaat atgatcatca aataacccct
     6361 gcttttgttg ttcttctgtt tgtactattt cattcattct tttctttata aaagattcat
     6421 ggcagataaa tcctgcttca ataatactga atttttaaaa tgaatgctgc tgatccccag
     6481 ttcctctgca gctatcacat ttctcaaatt atcatcaata aataatgctt ttgcagggtc
     6541 aacctggtac ctgtcaagca accgctggta aaactcagga aatggctttc tagttttctc
     6601 ttcaccactt actacccttc catcaaacca atgcagaaaa tcataacgaa ccaaagctat
     6661 atcgaataga ttggcctgcc agttcgtcaa tgcgtacatt ttataattac ccaactcctt
     6721 taaccgacga aatatatcaa ctgattctgt tatcgggcct ttaagcatct ccgtccaccg
     6781 gccataataa tcccggatgg ctttttccca ttcaggaaat ttttttacta attcctgagt
     6841 ggcctccaca atagaacggc ctgcatcctg ctcttcattc cagtcattgg aacaaatgtg
     6901 tttaaagaaa tattctctct tttcctctgt ttcaaaatag ctttcattga acaagtaacg
     6961 agggttccag tctatcagca ctccgcccag gtcccagata atagtattta ccgccattgt
     7021 tactggtgat tatacttcat tttcagcatg atcataatac ctgacattac cagcatcaca
     7081 gcattggtaa ggatgataac ccaattatca atcatcacac catataccag ccacatgatc
     7141 tcattaacaa acgcaataat gaacatgtat aatgaaacat cttttgcaga tttttccttc
     7201 catgttttta atacctgtgg caaaaaggta aaggctgtta gtgcccctgc ggcatagccc
     7261 agtatatcgg ctgctgacat tgacattatt ttttcttttt agatttggga agtgctttta
     7321 tttctttttt catttgtaac tgttgctctg ctgtaagtgg tatcatgctg atagcagccc
     7381 cgccaccagg agccagcttt aatcctagaa cagaattttt atttacaagc ttcttttcaa
     7441 tagtgtaagc ctccggatta tttttccagt ctgcattatc tgcatcacgg taaataatcg
     7501 cctggtacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn actttggttt catcccagtc
     7681 tgctgccaca tctttaatga actggagggc atctggtttt gcctcataat tttccggcaa
     7741 atcagcggcc atttgaagcg gactgtacat agttacatac aaggctaatt gtttagccaa
     7801 agtagtatgc acctgctcct ttttctccgg attgtaatga ctcatcttta ttttaaaaat
     7861 accaggtgta taatccatcg gcccgcccat cagcctggta aaaggcagaa tcgtttcatg
     7921 ctccggagga ttgccgacac tccatgcatt gaactcgttg ccacgggccg cttcactggc
     7981 tatccagttg ggataggtgc gatgaagacc tgtaagacga nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnncgctgt cttttgtgcc cctctttcat aatgctttat catccattgt
     8101 ccgtcatgat gttcacccct tggaatgatg cggccaacat aaccggtttt tactgcatca
     8161 taaccaaact tcttcatgaa acgataagct gtatccatcc aacgttcata attggtaaca
     8221 gaacctgatg tttcatgatg catgattatt ttcactccct tgtttgctgc atactgtttt
     8281 aattctttta catcaaaatc agggtaaggt gttacgaaat caaaaacatc ttctttccat
     8341 tgaccaaacc aatcttccca gccggtattc catccttcaa ccaatacgcc actaaaccca
     8401 tgcttagcag caaaatcaat atatcgcttt acattggctg tattggcccc atgaggaact
     8461 ttggtagctt gctgagaacc cacctgcccg ccactataat cccagctgga ccttcctaca
     8521 tgcatttccc accaaacccc gacatatttc ataggtttaa tccacgaagt ttcttcaatt
     8581 ttagatgggt cgttgaggtt caaaatcagt ttggatgtaa gcacctcccc ggctttgtca
     8641 ctcaccacaa tggtacgcca gggtgtgaca gccggtgcct gtaaatatgc cttattaccc
     8701 actgcatcgg gaacaagatg tgaattcagc gtaaaagtgt ttttatctac tacaaggttc
     8761 atagccgggt aattaaccaa tgctgcttca tggatattga tatataaacc atcggcagat
     8821 tttagcatca acggcgtttg tactgcagac ttatcgaaaa aagttttagc atgtatttcc
     8881 tgtgcagctc cgccaccggt tgcatccacc ccacttaagt tggttgtata ataggggtat
     8941 tcgtttgtat cataatcacc gggtatccag aatgctttgt gatcaccgga cattgcaaat
     9001 tgtgtcagtt cttcgccaat tataaaatga ttgagtttgg cctgttgcgg aaacgaatac
     9061 cgaaaaccaa ctccatcatt gtatactttg aaaataattt caagctggat accagaacct
     9121 gaacgatcag ccaattttac cagcagttgc ttgtagttat tgcggatttg actgacttca
     9181 ccccatacag gcttccaggt ttcgtctgtt acagatgaat cgattcccag caaatcaaac
     9241 tttgataacg acaattcagg acttttcaat cgcataccca atcccgacgg agctacaacc
     9301 gtttttcctt tataaaaaac cctgtatgta aggtctccgt tgctacgatg taacaaaccc
     9361 aattgtacat taccgtctgg tgagtgaagt gttacagact ggtcagcaaa aacagtagtg
     9421 ctnnnnnnnn nnnnnnnnnn naaaaacagg attggcagca atcgtttaag aagcataaaa
     9481 aaaatttagt gcatgaagat acaaactttg tgtaattggt acacattaga attatcgctt
     9541 ttcaaatagc caccagcgcc ttctccgggg cttcacctgg taattctccc ttatataatt
     9601 atgtatgtag ttgatagcct catccacact atctgtcagg tacaccagat taatgtcatc
     9661 tctggaaact gttccttctt ctatcatggc gtcaaccatt tccatcattg gctgataata
     9721 ttctttacca aacagcacta tcgggaatcg ggttatcgtt tgggtttgta caagggtaag
     9781 ggtttcaaaa agttcatcca tcgtaccaaa cccaccaggc atgataataa aggcataaga
     9841 atatttaacc agcaaaacct ttctaacaaa gaagtgctca aatgtgaccc atttctgcat
     9901 atacggatta gctttttgct caaatggcaa ccggatattg cacccgacag actttccacc
     9961 attctcaaaa gcccccctgt tggcagcttc cataataccg gggccacctc ctgtcatggt
    10021 ggtcataccc agttctgcaa tccgttttcc aatttccctt gcctgctggt aataaatatg
    10081 atcttcttta aaacgggcag aaccaaatac ggtaacacaa ggcccgataa aatgcaggtt
    10141 tctgaatccc tttaaaaatt gccagaacac cctgaatgca aaagataatt catatcccct
    10201 gcttttaggg ccttccagaa acacatgtgc ctttggtggt attattcttc tttttgattt
    10261 tgcggatgct tccatagtaa acactaaatt gccgtcaatt taatcaataa tgcaatgcgt
    10321 atcatcagct acaatgtgaa tgggatcagg gccgccataa aaaaaggttt tatggattgg
    10381 ttaaaaactg atcctgcaga tgtgatctgt gtgcaggaaa caaaagcagt gcagggcgat
    10441 gttgatcaca aaccttttca tgaactggga tttcataatt actggttcag tgcccaaaaa
    10501 aagggataca gtggtgtggc ggttttcaca aaaattaaac cggataatgt agaatacgga
    10561 aatggccatg gacccagtga tgatgaaggc agggtgatac aactggattt tggaaaaaca
    10621 aggcttatta atgcttattt cccctccggc accagcggag atgaaagaca ggtgttcaaa
    10681 taccaatggc tggatgagtt caacagctgg ttaaaaaaat taagaaaaaa acaccccaat
    10741 cttattttat gcggtgatta taatattgcg cataaagaga ttgacattca tgatccgaaa
    10801 gggaataaaa attcatccgg gtttttacct gaagaaagag catggatgac aaattttttg
    10861 gaagacggat gggttgatac ttttcgtgag tttcacccgg aaccacaccg gtatagctgg
    10921 tggagccagc gtttccccac cgtacggcnn nnnnnnnnnn nnnnnnnnnn nncgactata
    10981 ttaatgttac tgagcccttg aaaaaaaatc taaaagatgc ggaaatactt cctgatgtaa
    11041 agcacagtga tcattgtccg gtttacctgg aaataaaact ttagttctca tgatcattta
    11101 taacgttacc gtcaaggttg atcattctat tgcagaacca tggttagcct ggcttaaaga
    11161 ggagcatatt cccgatgtga ttaatacagg ttgctttaca catgccagca tattacgctt
    11221 attggaggta gatgaaacag atggcataac ctatgcagtg caatatcatt ctgccagtaa
    11281 agagctttat aaccggtata tcagtgattt tgcagaaact atgcgaaaca agggattcga
    11341 taaatgggga aataaattca ttgcatttcg ctcggtaatg atggttgtgc attgagtgtg
    11401 aaaaaataat cagccggtaa atgatacagg taaactgtgt tcccgttcta aataattgta
    11461 ttactcacgg atatatctta ctgaaagtca ggggcagtta tcggtaccat ttattattta
    11521 cccccaattt gaatactgaa atcaagtatt ctgctgaaat ccttgtccca tgcggattct
    11581 gaagcataag aacaaagccg gaaccgggta aaattatttt gcaggaataa aaaagcggct
    11641 aaatttgccg gcgttactaa accgcaaaaa aatacaataa tcatgaacaa agctgaatta
    11701 attgcaaaag tttctgacga tgcaggtatt accaaaacac aagcaaacgc cgccctcgat
    11761 tcttttattg aagcagttac aaaaacactg aaaggtggcg gtaaagtgac gctggttggt
    11821 tttggaactt tctccgtatc aaaaagagct gcccgtaatg gtcgtaaccc ccaaactggt
    11881 gaagtaatta aaatcaaggc cagaaaagta gcccggttca aagctggtaa agaattagct
    11941 gctaaactct agttgcctta atcaatgtat taaaaagctc cgttccccca tatgggaagg
    12001 agttttttta atcctgtatc tttacactta aacataaaaa aataatcatg ggaagaggag
    12061 ataaaaaaac tgctaaagga aaacgcttta agggctcatt cggaaaaagc agacccgcca
    12121 aaaaggctgc agtaaagaag aaagctgcta aaaaagctta atgaattaaa aaaaggtgtt
    12181 tgaaaatcaa acaccttttt tatttgcgtc ttatcccaac cagatcaggc attatattgc
    12241 ttctttgcta attgatctcg ttcttcagcc agtacattaa gcatactttc gagttcgccg
    12301 atacgtttca actgtccttc cagccgctta ttggcatctg ctactacctg catatcactg
    12361 ttcagctctt ccaggtaagt aactcttttt tgtaattgct gacgttgaaa attggcctct
    12421 ttcagtttat cttctgtttc cgccagatca gcctgcaatt gcctgttctc cgaaaccagg
    12481 tgattgtatt tatgcttttg ctcttcataa tcctggctta tacgatagta tccttctttt
    12541 aaatcttcgt actccaggtt gatttttttt gaaaccgcca cctgcgcctc cagtttctgc
    12601 attttctcct gcaacacccc aaactcgcta taggcactgt caagcatgga attcatttcg
    12661 ctggtcaggt tttctttctg acggattgca ttgatttcat tctccttctg tgccagctta
    12721 tgtttcagta actctatttc ttcgttcagc tcttcattgt actttaatac ttcctgttgt
    12781 tttgcctccg tttctttaac aatatctatt tgctccaaaa gctgattaat tttttcatta
    12841 tgttccagca aactgttttg agccagcttt aactgctcca tgtaacctgg tttttccccg
    12901 gaagactggg gtacctggtt tttcaatagc tctatttcct gttgcagttt tttgttttgc
    12961 ttgcgaacct cttctgcctc aatggagaag atgttactat tctcctctgc atcagccacc
    13021 agcttttgaa gattctcaat ttgcttatcc tttaattcca cttcattaaa aaaacgcctc
    13081 ttccaatcgt caagttcccg gctaagctta ctgccggcag catctgccat ggagtctttc
    13141 aatgattttc ggctggtaat aaaaaaatgg atggttatgc ccagaataat agccccgagc
    13201 atgagtacta tgatctcaat gatggaaagt gaaagctgac cgtccataaa ctatttcata
    13261 aggattagga aactattaaa tattcggcta atagccggta atagtttttc acaggtgtgc
    13321 ttaagaataa gtacgcaaga tagtagttac tgcaaaaaga aaaatagtag aaacacttag
    13381 tatttacccc cttacaagag tacctacctt ttcgccagta acaacccggc gaaggtttcc
    13441 ttctttattc atatcaaaaa caatgatggg aagattgttt tccatacaaa gcgtaaaagc
    13501 agtcatatcc attacccgaa gattattggt caggcattcc tggaaggtaa tgacattgaa
    13561 tttggttgcg gaaggatctt tttcaggatc ggcagtataa atgccatcta cccttgtacc
    13621 tttcaaaata acatcggcgt tgatttcaat ggcacgaaga gaacctgctg tatctgtagt
    13681 aaaataagga ttacctgttc ctgcaccaaa aatcactacc cgaccttttt ccaggtggcg
    13741 aatggcccga cgacgtatat aaggctcggc tatctgctcc atttttatag cactctgaag
    13801 ccgggtatat acacccactt tttccaaacc tgcctgcaca gccattccat tgattacagt
    13861 cgccagcata cccatataat ctccatgagc cctttctata cctgtttcag cttcgttcat
    13921 tccccggtag atatttccgc ctccgattac tactgctacc tgtacaccca attcggtaat
    13981 agacttaatg tcttgtgcat actgggcaat gacatcggta tcaaagccaa agcttttatc
    14041 acccattaaa gcttcaccgg aaagtttgag aagaattcgt ttgtatttag gcagcatgga
    14101 gcaggatttt tcgctgcgaa gatagggctt ttgcttattg ctttacagcc ctgatagtat
    14161 aaaccatcgg tagctttcct tccattcctt ttatatgcca ttttcccatt tcaatttcta
    14221 ccacctgttg aaaacaggga tatggggagt acatgaattc gttgaatgat gcaattgtta
    14281 acccggcgcc tatcagcgca tttagtactt cacttatact atggttccag ctatactctt
    14341 tacccctgat atccgcattc cggtcagtat aggtttcctt gctttctgtt acaattactt
    14401 ccctgttttc gtaagcatac tggatgtgtg taaaatcatc atcaaacatc catatcaccg
    14461 ggtgaaactc ggcgatataa aaaaaacctc ccggtttcaa catccggctt atcatattgg
    14521 cccatggttt aagatctggt aaccagccga tggtgccata ggaagtaaat acgatatcaa
    14581 attcttgctt tatgtaatca gctgtatcca gcacattaca acaaacaaac tcagcactca
    14641 ggccaagttc catgttcagt gaccttgctt ctttgattgc aatatccgac aggtcaactc
    14701 ctgttacatg ggcaccccgt ctggcccagt cgagactgtc cataccaaaa tggcattgca
    14761 aatgcaatag ttttttacca tttacatctc caacctcggc aagctcaatg ggagtcaaaa
    14821 cagtttcgcc tttcttaaac cctgcgagat tgtaaaactc cgaatctcta tgaacgatgg
    14881 tccgctggtt ccaaagctct ttgttggcat taaaatactg atcgaaattg ctcatgatat
    14941 taaaattagg aaaacacctt gtgtgtacaa gatgttttcc tttgtcaatc ggtaaaagtc
    15001 tattctttga ttattcttga atcgcctgtt gatacaattt gtgtaatcaa agggtctccc
    15061 cggtaataaa tacggctttg accagaggca tttgctttta aactattacc cactcttact
    15121 ttaatagtgg actgtcccgt agctgttacc tcagcaaaaa cgactggtga tagtccatat
    15181 gccagaattt cagattggcc acttgcttcc ccttcaaaac ccgtagcaac tccaccctgg
    15241 aattcaattt tagactggcc acttgcgagc aacttaaatt tgggtgcatt catattaatc
    15301 cagcaagcac tctgtcctga aacaacaaga tttaccagat ctgtttctga aaatccagaa
    15361 acagtggcct ctgactgacc agataatact aatccttcca gggaaggcag tgttatggta
    15421 aaataaactc ttttccggtt tgatttatac cggttatatt caatctttaa agaagaatca
    15481 ataacccgga taacgagatc attaacgtct ctttcttcac cccgggctat tatcgaaaag
    15541 ccagtaccct ttgttatagt aagccgatgc tgatcgccgg catcgatttt tttgaaatca
    15601 atatttgaat agcttcgctc aataggatta ctgggctctt ttgagcaggc agtgaacaag
    15661 aagaggcctg caaccaagcc gaaaaatact tttgatatta ctttcatttg aaaaaattta
    15721 atgattaaag attgctgaaa gaagaattat cccttcttag ccccgaaaaa gtatccaaca
    15781 cttactgata cagttctgtt acgaaactta tagtcgggtg catccccgct gatattacta
    15841 aacccatgca ggtaattggc agaaaagaaa aggccatttt tcaattcaac acctagcatt
    15901 cctgctccgc taaactcaaa acgcttaaag ggttgttcaa tatgatcgga ttttttaaaa
    15961 ggattcactt cgtatgaaaa aataggctca ggaatacctt caataaaact ttccgttttc
    16021 actttaccac caagtccaac tcccgctgat ggaccggcgc cgatgagaaa tgtagtactg
    16081 cccagctgtt ttttataagt aaacaatacg ggcaattcag cataattcat cttcacatcg
    16141 cttttaatag agctaattat tcccatttca ctggctgtag ctttcgcttt aattcctttc
    16201 cggataaaac ctaaagatgg tcttatacta aactcagacg aaagcgggat gtccaggatc
    16261 aaacctgctg caggagcata cactggtttg gttgtagtaa ccaccccctc ttcttttgtg
    16321 aaagatgatt tactaagtga agcgcttcca taaagtccaa ctctgatttg tgcatggcta
    16381 accataacac ctgccaatgc caggcataaa acaattcctt ttttcatgac tatactttta
    16441 tagattaaat aatacaagca aatatcattg atgctgtaac ctgaaccaac cgcatgaaga
    16501 tgtgaaaaaa gaaaggccct ctttagaaaa agaaggccgc taatgattgc ttatgtagga
    16561 gatttttgat tcaataagag attcttctat tacaaatgtc tgatacgaaa tattttttcc
    16621 aatcacataa acatgtgaat aatgattctt ttttaagaag catcagaacc gccaaataca
    16681 agccaggtgt tcacgacagt atcattatca ccttcacatt tgttcgatac ctcgacacaa
    16741 tcagcatcga ttaccatttt tggatcagtt tcctgtttcg gtttttcagt ggcgaggcag
    16801 gccagttgaa tattccgggt gatgtatttt cctttcataa aataagttta atggtttaag
    16861 gtttttgttg atgcaaagat catagaaagg aaaaaggcag acaatcacat attggtgtga
    16921 agaatgttta aaaaacaaaa aagatgtgag aaatttccca catcttctgg caggcccggc
    16981 tattatatta ttccccgtat ccgcttttaa gcaaaacctc ccggggagat aatgagtaaa
    17041 tatctttcca gctttctttt ccatgttttt ttacatacgc ttcacatatc ctgaaaccta
    17101 cgaaataacc aatggcaccg ggcccctcct taaaaggttt ttgcccccga tcagccaatg
    17161 caagtgcatg ctctctgtta ttggtaaaaa ggtatggctt tagcttgctg aatatnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgaagtccg gagacagaat actgtaaata
    17281 ctcatgcagc tcatacttat cgccaaatac cacctggttc atatacacgg caaatccttc
    17341 attaatcacc cggtataagc cccgggcagt cgtatcgggc agaattgtat tttcataaat
    17401 ctggtgattg atctcatgtg gcagaatcat ctgcacataa ccaatttctg ctttactatg
    17461 tgtcagatca agtaccattc gcccattacc aaagccgccc atatcagtta cgccactacc
    17521 aaaggcaata aaccatgtgc cggaaggtgt tctgccactg taacaattca aaactgatgc
    17581 ggtaagatta aagaagttat caatattgaa agatgaaaat gtcaacgcct ttttagccat
    17641 tatgcgtatg ctgtccttaa tcagcggctt cattacctgc tcataataaa cagtactatc
    17701 acctacatag ccttcccaaa attcttgata tggcaaatag gttcttttga ttagttctgc
    17761 tctaacttgt tgctcagtca ggtttcttgt ttcatacaaa acagcaattt gctgtttata
    17821 aatatttata agcctcaggt taccgttaaa gagattgaca ctatccttta ccttagtaac
    17881 acgataatag gtttccccgt ctatttctcc ttctgcatag gcatgtagca aacccaggga
    17941 cattaacaaa acaattaata actttctcat attctgcaaa ttgattattg actcattcat
    18001 atgaatgctg gaactcaaac tcattcgttc ctgtaacatt tatttttcct tttgaccgta
    18061 aggaaagctg gcaggcacca tttaccacca catcaagttt cctgttatcc agcacctctt
    18121 cggtaacaac cttagagtct ccattaataa caaactgctt caataaacgg gcaggcacat
    18181 ataccgttaa tttatcgccc atgttacttc cttgcagcca tattgatagt ttacctttct
    18241 taacaagggc ctttacttta ccaaactttg acctgtctcc gtctactgaa atatcctttc
    18301 cggtttcgtt ggttaataca acagaaatat gatcacttac ttccaggtaa gtaaaatgtt
    18361 gctccagttt aatgcgcatg acaggcggca aatcttttcc gtatgtaatc cctgttatca
    18421 taataaatat ggtgactata cttatggtcc aaaaattcag tttcataatt ttcattttat
    18481 aaggttatta atcaattata agtcaaagat catttttaaa tatgccggaa aannnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnngtctgcg aaaattgcac tggataataa tgaatggggc aaagcagaag
    18781 aatttgctgt gaaaggaaga cagggtatca tgattaacca gaaactgagc tttggcagtt
    18841 accgcacagt ttctgtaaaa agatcatgga ccaaaggaag cagttacttt gccggttggg
    18901 ctagcggaag acccggctat gatgattacg aaagagtaat cggcactgag tattcacagc
    18961 gcaaacaaac tatccggttt gagatgactg atgataaagg caatgaaagc agtgtttttt
    19021 gcctatcccg gacaagagaa aaggatttta tgattggaaa caatcccaat agcctgatta
    19081 atattttgct ggatatatct ggtaagggcg acatttcaga aaacacattc tggataaaaa
    19141 tattcatcaa taaccagtcc aggtcatggg aaatgttact tgataatgaa gctgctcaaa
    19201 gaaacagcaa atcttatact ggagtagtgg cacaaagccg cgacaattat tacactattc
    19261 acccaatatt taaaatgaca ggtaagaatc aaaaggtgta tacaatgcct gttggttctg
    19321 ttgggttcga gctaagaaat aaagaaggaa aaccagttgc tgcagtatca gctattgata
    19381 agggatctgt ttacttaaac agtactgatc cgggtgaaaa attcctgctt tccaatatct
    19441 gtgcagctat acttcttagg gaatcaatat aggatcgaag gccgggaatc aaatcccaca
    19501 taaatatgtg gttttaaaaa gcgatctttc atatgccggt tccacttgtt agatttgtat
    19561 atatgagaag actgatgctt ttagctgtat tgcttaatgg gctttatgct catacccagc
    19621 aaggttatac atttaatcgt atcagtacag acgatgcact aggtctttct tccaacgcag
    19681 tatattgtac ttaccaggac aaaaagggtt acatatgggt aggaactgcc aatggcttac
    19741 aacgctttga tggcagcaag tttgtaagtt ttggaaatag tgacccgggc agcaccttat
    19801 tacccatttc agaccttgtt caaataattc cggatgacaa tggaatgctg tggttgtttt
    19861 ttggctccag gcttgaagta ggtttatttg atccgatttc cttccgctac catacagtac
    19921 ccataaaaac ttccaaaccc atacccgcca ggagcggcat gaagctttgg aaggatagcc
    19981 agcaaaatct ttacctggtt atctggaaat tcggaatact tcgctacaat aaaaacaagc
    20041 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn gacagtggaa tagccattta tgacattctc tcaaaaaaaa tatacaccag
    20221 cagcaataat ccactaaaga tccccttgct ggacaataaa aatatcatac ccggcacaac
    20281 tgaaattttt atcgatagca gaagaaggca ttggatattt aactggacca gtgggcagca
    20341 taaatggtgt tttgatgaaa aagggaccct cctaaaagat acagcaggac taaacgataa
    20401 cagggaatat tcagagctta ccagtttttt tgagacaaag gaaaaagtat tatgggtata
    20461 tggagcaaac ggtttattta attatgacag cgatgtaaac cgcttctttt tttacaaacc
    20521 tggggtaacc agtgaaaccg gaattcctta tcagtctgcc cactatatta tggaggacag
    20581 ggatggaagt atctggatat cgacagataa tgggctcttt ttcgccaccc ctgaaagcgg
    20641 cacttatggt gtggtgaata ttctttttga tgaaagaaac ggagggatag aaataacgga
    20701 tctgctgcaa ttaaagtccg gtcaatattg gctaagcacc tggggcaaag gaattattac
    20761 attagataaa acctttgcga aatattcagc tgatgtatat aagctgatgc cgaaaatgga
    20821 ccaggtcagc tttgcccaat attatcaaac atgggcttta taccagcata cagatggaaa
    20881 aatttggatt ggctgccagg ccggtaaata cattatttac gacacattaa cgcaaaaatc
    20941 atcatttgct gaattaaaag aggccgatgg cgccacaatt cgatttatta ccggggataa
    21001 gaacgggaat atctggtttt ccactcaacg aggacatatc attaactatg atgggaaaaa
    21061 attcactgta gttcagcagt tcggtactat tatcccaaaa atattaattg acaacaatgg
    21121 attgttgtgg ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnacgatat tgatcagatc aatgatacaa ctattgctta cggtgcagga gccctgaacc
    21301 ttatcaatac aagaaccggg aaagtaacct ggcgcacatt tgatgacggg ttgccgggca
    21361 attcaataag gcgtatacgg acagacagaa atggcaacct atggatcatc acaaagaatg
    21421 gattgagtaa atataatccc cggacaaacc gctttacacc ctatggcaga aaagatggca
    21481 ttacgctggc taacttcact actgccgctg attttatnnn nnnnnnnnnt tatactcttt
    21541 gccggatcta acgggctgat gtattttatg ccttcccttt ttgaaaataa aaaacctcct
    21601 gccaatgtag tcattactga ttttaaatta ttcaatgatt atcttccact ggattcactg
    21661 cttaaacgac ctaaactgga gtttaactca gatgaaaatt ctttcagtat ttacttttct
    21721 tcactcagct attcacaaaa agagaaactg acctactatt ataaaatgga aggagtagat
    21781 aaagaatgga taaaagctga caggcagaat tatgtaaatt atccgctgct gcctcccggc
    21841 cattatactt ttagtgttta ctgtgaaaat attgatggca tacgttcaaa agagattacc
    21901 tcatttgaaa tctatatcaa acctcctttc tggaaaacct attggtttat aagcaccctt
    21961 ctgctgatta tcctgctatt tatctatgcc ttacacaggc tgcgggttaa taagttactg
    22021 gccgttgaaa agatcaggaa ccgtgtagcc agggatctgc atgatgatat gggatctacc
    22081 cttagcacta tcaacatact cagtacaatg gcaaagagta aactgaattc tgataacgta
    22141 aaaacaagcg aatatatcag taagatcagc gagaacagcc agcgtatgat ggaagcaatg
    22201 gatgatattg tctggagtat aaagccgatg aatgacagta tgcagaaaat cattgcaaga
    22261 atgagggagt atgccactaa cattctcgag gcaaaagaca ttgaagtgtc ttttaacata
    22321 gaagaagagg taaatgatgt aaagctggat atggaggccc gcagagactt cttcctcgta
    22381 tttaaagaag ctgttaacaa tgcagccaag tattcaaaag ctgaaaaagt taagatcaac
    22441 ctgtttattt taaccgggca gctcactctt gaagttattg atgatggtga aggttttgat
    22501 gtggcccttg cagacagcgg aaatgggctt agcaatatgc agaaaagggc ggatgcgatg
    22561 cagggcagat tgcagattat atcaaaaaaa ggaaatggta ctattgtaaa acttgctgtc
    22621 ccattcaact aaataataac catgaataaa accttaagcc agaaattccg gttcttcaca
    22681 tttatctgca tagcactgct ggcttatgta catggctata acctgaataa tacttatctg
    22741 gcaccttata gcacggtaga agagccattg actgttacct ctttttttga atactttata
    22801 gcaaacggtc tgctcaggtt caggatacca atgcttttta ttatctcggg ttatttgtat
    22861 gctacctatg atcaccggcc ctacctgcag caaatacaaa aaagattcaa aacgttaatc
    22921 attccttttt ttctctggag tgcagcaggc cttgctctta cttttctatt gcagcaattt
    22981 acctatactg ctgacttggt acatacggca ggcatagacc agcttgggga taacagacct
    23041 tatactgaaa ttggctggga agggatactt agaagatggt tactacatcc tgtgtcctat
    23101 cagttatggt ttattgtttc tcttttcatt tataatctaa tttatccagt tatcaggtgg
    23161 ctggtggtaa aaattccttt tatctggtta ccgctgactt ttttgctttt cttttcgctc
    23221 ttccaattta tttatgttga tggcaggggc attttctttt tctcattggg aatttggctt
    23281 caaaaaaaga aaatatctat tgataaacaa ccggaatggt tcagtgccgg cctggccttt
    23341 attcttttca tagggctgac ggtaattaaa acatttatgg ctttcgaact tgagcctaat
    23401 cagttttcaa ccagccttac cctggtaatt ttttatcaat tatctgttat cacaggaatt
    23461 ctggcaatgt ggtttggact ggataaagta gttcggtggg cactaaggaa gaaatggttc
    23521 tacaaagcca gcggcttctc cttttttctc tatggagccc atgtacctct tttaccatac
    23581 gctatgacca tggccttaac ggaactgagc tttttgccat attacaggct attctgctat
    23641 attgtgatac caggactgat tgtcgtactt tgtatctgga taggcaggtt tgtgcgcaaa
    23701 tttctaccag gtatctattt tacccttaca ggaagccggg ggttctgata agccggctat
    23761 ttcacattaa tatgtgatag gataccagta gcaaggttgg taaatttgta taaatggcta
    23821 taattcatcc tttcaacttt atactatgat caagataatg ctgtatgaag acaacccgca
    23881 gcttagagaa ggtttaacga tgctgattga tggcagcgag ggttttcagg ttttggcatc
    23941 atttaaaaat tgcagcaacg tagtacagga agtagaggct tttaaaccag atgttattct
    24001 gatggacata gatatgcctg ttgtaaacgg cattgaagga ttaaaactca ttcgccagca
    24061 taaccacgat gtaaaagtcc tgatgcttac cgttttcgat gataataaga atgtttttga
    24121 agcattaaaa aatggagcca acggttatgt attaaaaaag acacctccgg ccaaactgct
    24181 ggaatatatt accgaagcgg ccagcggtgg cgcacccatg acctcatcga ttgccacaca
    24241 ggttttaaaa atgttttcgg aagtacaggc ccctccgggt gaagactata accttagcga
    24301 cagggaaaaa gaagtattac agcacctggt aaatggttat agttacaaaa tgattgcatc
    24361 agaaatgttt attgccatcg atacggtaag aagccacatc aaaaagattt atgaaaaact
    24421 gcatgtaaat tctaaatctg aagcagtagc taaagcattc aaggacaaga tcgtctaacc
    24481 acactcaaat aaaataattg ataaaagatg tgactccctt tcacatcttt tttatttccg
    24541 gaaagcctgt ttcacatatt tatgggattg aaataaggca tctgccattg catctttact
    24601 ttataatcaa aaaacatcaa ccattaaacc ttatttttat gcgtacaatg attctgttgc
    24661 tgtcagttta tattgccggt atagcaatag ttacgacagc ctgtaaaaat tcctcaactg
    24721 gcaaccctcc cgttagtgaa aagaaaaatg attcatctga aattgtaaaa agaggcgagt
    24781 acctggttac tatcggtgtg tgtgatgact gccattcgcc taaaacaatg ggagcaaggg
    24841 gaccagaaat tgatatggag agaaggttat ccggtttccc gggaacaagg cctgctcctt
    24901 cctataacaa ggagttaatg caatctggta ttgtacaatt caacgaagat ttcacatcgg
    24961 cagccggtgt atggggtatt tcttttgcag ctaaccttac cagtgatgcc acaggcatag
    25021 gtaactggcc gctagagaac ttcaaatatg ccatcaggca tggaaagctt aaagggatgg
    25081 aaactggccg tgacctgttg ccacccatgc catggactaa tttctctaaa atgacagacg
    25141 aagatattga ggctgtttac acctacctga agacaattaa acctgtaaat aacgtggtgc
    25201 ctgcaccaat accaccagca ggtggcaaat aataaggtaa agtcatgaaa aaagggagct
    25261 gcaggctccc tttttgtatt taactattgt aatattaacc taatgcaacc ctcttgaatg
    25321 aggtcacttt cacatcaccg ctgtctttca ggtactgctc aactgtttta cttccatcct
    25381 taacaaaagc ctgtgcagtc aatgtttgtt ctttaaagaa agcacccatt ttaccttcag
    25441 caatcttagc cagcatttca tccggtttac ctgccatctt aggatcttgt ttcatcaatt
    25501 ccataatgat gtctttttca cgggcaataa cttctgcagg tactgaagct gcatccactg
    25561 ctaccgggtt taacgcagcg atctgcatcg caacatcttt tccggcttcg gccgcttcct
    25621 tatccatccc taccagcaca ccgatgcgat atgcgccgtg aatataagat gccacaaaag
    25681 gagcatcaat tctttcaaac ttggtgatac caattttttc gccgatagtt gccagttttt
    25741 catctaccag ggcctttact gtaagatcac cgatcttaac ttccatcaac tggtcaagat
    25801 tggctacatt attggcgata gctgcatcag caatagactg accaaatgca acgaagtcag
    25861 cattctttga aacaaagtct gtttcgcagc taacacaaac aacaatacca gttttattat
    25921 cagcggttgt tttagcaata accacaccct cttttgcctc acggtcactt cttttagcag
    25981 ctactttctg accctgctta cgcaaccagt caatagctgc ttcaaaatca ccgtttgttt
    26041 ctgtcaatgc tttgcggcaa tccatcatgc ctgcaccagt agcctggcga agtttattta
    26101 tatctgctgc acttattgtt acagtactca tatttattta tttaaagttt aaggtttgag
    26161 gtttactact gtgttcctca gctccttagc gtaatcaaat tttaaaagct ataccgggaa
    26221 agaaaagcca tataataaca actaaaaaaa cccggctaat ttatctcctg gcaccaccgg
    26281 gaccacggct gccaccagca ggtgtgcggc gtctttgtcc accaccacca gctccacgac
    26341 ctctgccacc acgaccacct tcagcttcgg cttcggccag caacttagct gcttttcttt
    26401 ccttttcatc atcggtcatt tcttctacct cttcgtcttt tgcagcttgt ctttcagcta
    26461 atccctcagc gattgcagca gtgatatagt taacgatgat agcaattgac ttggtagcat
    26521 catcgttagc gggaatggca aaatcaactt tgtttggatc acagttggta tctactacac
    26581 caaatgtaga aacccccaaa cgttttgctt ctgccaatgc gatatgctca tgcccgatat
    26641 ctacaatgaa cacagccaag ctgagcaata ccacccagta ctttctccat tttatcttta
    26701 tcgcggctca aggtgaggcg ttctttctta gtaatgctgt cgaaagagcc atcgcccagc
    26761 attttctcaa tgctctgcat tttcttcaca cttttacgaa cagtgttaaa attggtaagc
    26821 atgccaccca gccatctttc tgtaacgaaa ggcatgttta ctttctttgc acattcgcta
    26881 acgatgtcct tcgcctgctt tttggtacca acaaacagga tctttttacc actgcgagca
    26941 atttgcttca gggcagcagc agtttcctgc aggcactcta ctgttttgtt caggtcaatg
    27001 atgtgaatac ctttcttctc agcaaagatg taaggcaaca tcttagggtt ccacttcttc
    27061 ttgaggtgac caaaatgaac acctgcttcc agaagctgct gttgtaagga tgtattattt
    27121 tccattgtat ttgaatgatc gtttttaata attgtttgtg gcgattaacg tttagagaac
    27181 tggtagcttc tacgggcttt tttatgacca aatttcttac gttcaacaga cctcggatcc
    27241 cttttcagca atcctgccgc ttttaatgca gggcgaaact caggattcag ttcaaccaac
    27301 acacggctga taccgagcat agctgcttcg gcctgacctt ttaaaccacc accggcagcg
    27361 ttgattttta catcaaattt gttcattaca tcagctgttt tcagcggacg ttccacctgg
    27421 tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27541 nnnnnnnnnn nnnnnnnttt gcttttccat tatttattaa ttaggaattt tttgaatgat
    27601 gattaaaact tcagttcttt cggctgctga gcagtgtgcg gatgttctgc acctgcatat
    27661 acaaataatt ttttgtacat cttgcggccg agccggttct ttggcaacat accctttaca
    27721 gccctttcga taacaacctc tggacgacgc ttctgcaggt tggcagcagt ttcttccttg
    27781 cggccaccgg gataaccggt gaaatgatca taaattttct gttccagttt gttacctgtg
    27841 aaaacaactt tctcgcagtt gatcaccacg atataatcgc cggtatcaac atggggagtg
    27901 taagaggcct tgtgcttgcc acgaagcatg gcagcaatct ttgcgcacat acgaccaacg
    27961 gtttgattag taccgtccac aacataccag ttgcgcttaa cggtagcctc gttcgcatgt
    28021 ttggtagtga aatgtagttt gctcattttt ttctttttta atgttatccc ttttcatcct
    28081 gagcggagtc aagggtagtg tcgccgctat cccggcaact gtttttaaat ggggtgcaaa
    28141 gataggtctg agcagaatgc cggcctacaa aaagaggaag taatttttat cgtacctgtg
    28201 tatatgattg attttcaata aaatttttgt tctaaaaaat aaatagcccc agtaggatag
    28261 ttaggaagct aaaccaatca gccgtttaat tacaatcctt gtatttgaca aatggctgat
    28321 aagcacggga attatatctc cttccttaat agaaccagtc tcagcggtgt caaccggcag
    28381 ctttgcctgg attttatcat caaaaagatt gaggtaaatg ccgtgtggag aaactttata
    28441 aaccacagca tcaatggcaa tttcattttt aaaatgctga actgcttttt tatacgctgg
    28501 tatataattg tgcgaaaaaa gttcggccac aatgctatta ggccggtcta ggtttatcag
    28561 gcaaaagctg atggcctcat tctcggcagt ttcaacaggt ggtttttccc agggagacaa
    28621 ttgaagcgga aaaaatatgt aactattcca ttcttttaca aagtatttgt ctccggtctt
    28681 cttcaaatat cctgagaaac ggttctcaat tgctgctctt tgtattaaaa gatccagctc
    28741 tgtgttatac aggtatttta tattacaggc tgtcatctta tcaccacgat cagagagtct
    28801 taactggaaa cttaccgtat cacccatcct gaaatgatga gattttctgc cagttgattc
    28861 aaagccagtt ttacagttta ctgatttttt cttcatcccc tgcaaatact caatagtggc
    28921 aaaatttttc tcgtaattga caaacgaaat ggttcccttt agtatctttt cctccataat
    28981 gctagttttc cttcccggta aaggtatgca tgatgtcaat tagcctggcc ggttttaatt
    29041 ttcaattgaa tgcacatgtc aaatcgaaat ttgggccgct ccgtatttgg tgatccaaaa
    29101 aatgccggca acctgatgcc cctagaagag gcgcatactt tattagagga atgggtaccc
    29161 aatgaaaaac tccggctaca tatgaaacag gtagcagcta atatgaaagc ctgggcgata
    29221 gaaaaagaag gtgccgatga actaaccgcc caaaaatggg aactggcagg tttgctccat
    29281 gatgcagact gggaaaaata tcctgatgat cattgccgta tcataattga ggagctggaa
    29341 agaagaaaca ttgaccctga tgtgatacat tgtatcgcct ctcatgggcc ttctgttttt
    29401 ggagtagaac cggtaagcaa aatggataaa atgatctacg ccattgatga gttatccggc
    29461 tttattcatg ctgccgcact tatacgacca actttatacg aaggactgga agtaaaaagt
    29521 gtattaaaaa gactaaagac tccttcgttt gccgcccagg ttagccggga ggaaataagt
    29581 gatgctttat caaaaacaga tacaacattg gaagaactga ttcaattcat catagaccat
    29641 caaaaatcag ttgtctaaaa tttttctttc cattttttaa acccggcaga ttaaacaggg
    29701 tcacagtgca gttaaaagcc tgcctgagag attaacacta ttaatccgaa ttttttaata
    29761 cgtcaaactt gcaggagata ttctgcaatt aggttgactt ttttctcgtc agggctaaca
    29821 aacaaagccg ttcctcctgc tatgagcaag gttggagcgg cttttagtat gacccattga
    29881 ctttaagcct ggtataattg taacaactat gttttcttct tcctggccag caaaagcata
    29941 attacaccaa ataccagcat gagcaaccct gaccaaagat ttacattata ttgaaatgat
    30001 tttttataga gttctgtatc agcattggtg attaaaccat atgcagttac cagtaaaccc
    30061 aacactgtaa acaacagccc aattggaatt cttatatcta acataatttt tgttttaaag
    30121 ctttaccaga ataagattga taagataacc atcatggtca atacaataat ggcaagtgta
    30181 ttggtccgtt tatgccaggc cacatggtct tcctttattt tgggtgtcaa cgagtatact
    30241 aaaccacgca actcgtcatc tgtttttctt tgagcagtgc tcaatgaaat tccaactgca
    30301 attatggctg ctgatgcaca ggcaaaaata gctgtccaga aactctgagc catttctaca
    30361 gggtaagtat gtatcactcc taaccagcct ccttttacca aagttgttgc accagcagga
    30421 acagttaggc catggtgcag caatgccacg aagaaaccac ctaacaaacc tataaatgca
    30481 ccatgcccgg ttgctctttt ccagaacatg cccaggaata ttacagcgaa aagcggtgca
    30541 tttatcatgg aaaaaatcgt ttgtaaaaag tccatgatgt ttccaaacaa acctgccagg
    30601 tatgcggctg caatacttac caaaacacca aatacagtag ccattcttcc caccttcaga
    30661 taatgtttgg catctgcctc tttatcattt gtggccggcc ttatataact ctggtaaata
    30721 tcataagtcc agaccgtgtt aaaagcagat acattccccg ccatacctga cataaatgag
    30781 gctagcagcg ctgtaatacc caaacccagc acaccggttg gcaagtaata ttcaagcagc
    30841 ttgataattg tcaaatcata attagccttt ccattttcca ttatggtaaa cccagaatct
    30901 gctttattca tcagcaccag cgccacaata cccggaacga tgatcaggaa tggaataatc
    30961 attttgggga tagcgccaat taatggtgta ttacgggcag ctgttgttga ctctgctgcc
    31021 atagcccttt gcacaatcag gaagttcgta caccagtaac caaacgatag cacaaagcca
    31081 aggccaaata atattccata ccattccaca cccatcgggt taccatttgc agagccagtg
    31141 tacttccagg tagttgtaaa tgtgtctcct gcaaatccct tttccgttac aatcggtgcc
    31201 aagctttcct tcaatccgct ccaccctcct acttcatgca agcttaatac aacgatgggc
    31261 aacaaaccaa ttactatgat aaaaaactgc aatacttcat tataaatggc agaagacaac
    31321 ccaccgagat aagtataggc taatacaata gctgcagaca gccataaact cagattgaaa
    31381 tcccagccca gtactttctc catcaacaat gctagtgcat acatcgaaat acctgacgcc
    31441 aatactgtca ttactgcaaa cagaatagca ttgagccctc ttgnnnnnnn nnnnngtagc
    31501 ttcagatatt ctggcacgga tcgggctttt gaaccataat agaaaggcat catgaaaatg
    31561 gcaaggaaga ccatggcagg aatagctccg atccagtaaa aatgtgtggt aagcattccg
    31621 tacttggcac cggaacctgt catacccata acttccagag ccccaagatt ggtggatata
    31681 aatgccaacc ctgctaccca tgccggcatt gatctgccag cttccaggaa tgcgttagca
    31741 tccttcatat atctcttaag cacccaaccg attcccagca caaacaagaa atagatgatc
    31801 ataatggcat agtcaatcca ttgcagatga aaaattgaat ttcccattgg taagttttta
    31861 aaaaatttaa tcttatcggc ctattacaga cgttccatct tctatattta ccaggtagtg
    31921 ttttaactct ttacccatat ccttcttata atcctccgaa agtttagcca caagctcctc
    31981 aaccatgtcc tttgaaataa tattaatggt acagcctcca aaacctccac ccatcatcct
    32041 ggcaccaatt actgccgggt tattccttac attgtcaaca aggtaatcca attctttgca
    32101 actcacttca tactctttgc tcaaaccatc atgcgttgca aacatttttt tcccaaacgc
    32161 aatgaggtca ccagcttgta aatcttcaca tcctttttgc aaacgatcaa tctcttccac
    32221 cacatattta catcgccggt gggctgtttc aaattcacca atacactcat taaccatttc
    32281 catagtagca tctcttaagc tatgtactga aggatagttc tgcccgatca gcattacacc
    32341 ttcttcacat tgagaccggc ggatattgta ctcagatgac ccaagcgaat gttttaccat
    32401 cgtatcaaaa agtacaatga catattcacc aagctccagc ggaaaatatt catactctaa
    32461 tgaacggcaa tccagccgga tcacatggtc tttctttcca aacaagctgg caaactggtc
    32521 cataatgcca catttaacac cggcatattg atgttctgca gcctgtgcca ttaaagccag
    32581 ctcttttctg gaaaaacctg actgtaataa ctcatttaaa gcaaaagcaa ctgcacattc
    32641 taccgctgct gaagaggaaa gaccggcacc taaaggaaca tcaccataaa tgcttagctt
    32701 aaaaccggta acaaattgac cctttcgaat aaactggtca actacaccga aaatataagt
    32761 ggaccaatga cccggaactg gttttatatc tcttatttcg acttctatcg attcattaaa
    32821 atccacagac tgtaagttca atcttgtgcc tggatttttt tctactgtta catacacggc
    32881 tttatctatt gctgcgggca agacaaaacc ttcgttatag tctgtatgct ctcctatcag
    32941 gttaatacgt cccggtgacc gcactacgat agtatcagca gtaataaatt gttttagctg
    33001 atccggtatt ttataatctc tcacttaatt tattttagtc ctttagatat ttattgctga
    33061 tctcatcgta caacttcagc aaatcattcg atttgccttt gtcatcaaaa aatttctccc
    33121 aggtactcaa tggctcccag atgcaagtac cttttcccct gccaccaggt acttcaaagg
    33181 caagtttgtt tacttcttcc ttccggtgag aatattccac tacaatcaca tctttgttat
    33241 acctgcggga aaggtcttcc aaattggttt ccaggtcttc cagggttcca tgccatttcg
    33301 gataatacga ttcaccaatt acatcaaagt ggacaccccg tgccatcata ttatcaataa
    33361 agaaaacgga ttcatcattc tgcccgccta aagccacatg caacatcata attactgagg
    33421 ggtcgatatc ttttacagcc tttgtaccag catttatcaa ttgtgcaagg ccatcaaaat
    33481 tgctgacatt tccctcgggc cagataatgc catgattaat ttcattaccc acctgcacca
    33541 tatcaggagc agttccctgt tctttgagtt gctgcatcac ctccttggtg taattgtaaa
    33601 cagctttctt caattcttca aaagacaagt ttctccaggc cgctggcttg tattgctttc
    33661 cgggatcagc ccagtaatca ctgtaatgaa aatctaataa gagcttcata cctgctgctt
    33721 ttaccctctt cgccattttc tttgtatttt ccaaatcaca aaaaccttta ccgggagaat
    33781 aaccactatc tcttgcaggg tcattaaaaa gccggagtcg tacataattg aaaccgtgat
    33841 tcttcaatat ttcgattgca tctttttcga ccccattatc ggagaacttt atacctcttg
    33901 cttccaactc aggcaggaat gaaatgtcag caccaagcat cttgccaact tctactggct
    33961 ttgattgttc aggtgtcagc ttatatttag aatcaaaaga aattttattg acacttccgg
    34021 gtgatatggt ttgaatatct gttcctcccg gccataaccc tgtagccttt gcttcaaact
    34081 tgatcattcc agtttcagta ccggcctgaa tgattacctg gcactttcca ttgaacaagg
    34141 ttcgctgcca tatgccattg gtagacttat cgggttcgtg actgcttgga tcgccattac
    34201 ctactccaat tattttaccc gggccttcta tggaaaattt gatgagattg tctgcatcgg
    34261 gaacttctct tccttctctg tcaagcacag ttatgtttaa aacagttgca tctttaccgt
    34321 cggccagaat ggtggtttta taaggtgtta ccaccacttc aaaaggcaag ccggttgtct
    34381 ctattttttt ataaagcctc tttccttttt tatatgcaac cgcctccagt ttaccgggtt
    34441 cataattcac tgaccattta agatggctgt tgcgtggcat atcttttttg cccaggcttt
    34501 tcccatttag aaacaattcc acattatctg cattagtatt tacccacaca tctatgagat
    34561 cacctttcgg cttattccag cctggacctt tctccctgaa attccaatgt ggtgacatat
    34621 gtaatacatc tttatctgtc caccagcttt tatagtagta ataaatgttc ttgggaaaac
    34681 cacacatatc catcaccccg aaatgtgaat tgatatttgg ccattgatat ggtgtgggtt
    34741 cgccccgata gtccatccct gtccaaataa atccgcccaa ccaataatca ttattggcag
    34801 ctaatgtcca ccagtcttct gcccggctgg cccaccaggg agctgttatg tcctggtcgg
    34861 gtacataccc tcttatcgta tccttttcat aaatacctct tgtagtaact gtacttccca
    34921 tttctgttcc aataattggc tgttcaggat ggtcacgatg gtaatcagca acagcaaatt
    34981 gcctgtaatt gaaagaacga accgggatta cttcattgat accgttgtat acattaggta
    35041 aatcagctgc ataggtacaa gttcttgttg gatcaagttc tttttgcttt gcaataaatg
    35101 tctgcgcaat tctctttcca aaactgttgg tatgtatcca cccttcttca ttacctatag
    35161 accacatgaa aacagacgta cggctacggt ctctcctaat tagtctttca aactgatcca
    35221 tatactccgg actgctgttg agcaaacgct gttcatccat taccagcata cccaaactgt
    35281 cacaggcatc caatagctct ggcgttgggg catgatgact ggtacgataa gcattcactc
    35341 ccattctttt cagtaagctg attcggtaat actgtaagta atcgggcaat gcactgccca
    35401 caccggcatg atcctggtga ttgttcactc cttttaattt cacatgttgt ccattcaaaa
    35461 aaacgccatt agattttacc tctatctggc gcaggccaaa acggatttgt ttccggtctg
    35521 tcaacctgtt gtctgacctt agctccacaa ctactctgta caaataaggg tcatcaaggg
    35581 tccatttcct gaaaccagta acagataccg tattaataga agtgccggcc ttgttgactg
    35641 caagagaaag agatatatct tttgacctgg ctacaaactt tccgtcccgg tctgttatat
    35701 aagcagatac agtagttact gtatttgtag ttgaattatt ctgaactaaa gattcaatcg
    35761 ttacggttgc tttattgccg gcaatagcag accatgcaaa tatgccgtct tttgctatat
    35821 gtacattact atacttattc agccatacat gacggtaaat accggcgcct tcataaaacc
    35881 atccttcata atgcgtggca tccactctta ccaccagcac attatccccg tagaaattga
    35941 tataatccgt aacatcatat gcagctccca cataaccgct cttatt
//
LOCUS       DAORLS010000005        34664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_644_length_34664_cov_2.285995, whole genome shotgun sequence.
ACCESSION   DAORLS010000005 DAORLS010000000
VERSION     DAORLS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 34664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34664
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_644_length_34664_cov_2.285995"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK977_00930"
     CDS             <1..498
                     /locus_tag="PK977_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16690.1"
                     /translation="PVEKINSLNQVCMKKQIVLSLLSVILFASCGNNKADQPTETNDS
                     STILYSWEATLNDSTGRLEVVKREGLSPDSLSVQAVIDFLNRKNQFVQIQYSKTSGDT
                     VFVGIPEATYLTQQMGSSGPTVFFAEAVYNLTEIPGIRFVNFDFEEGDHASPGVFDRN
                     TFKDQ"
     assembly_gap    572..618
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<619..>981)
                     /locus_tag="PK977_00935"
     CDS             complement(<619..>981)
                     /locus_tag="PK977_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRF16691.1"
                     /translation="QALQPSVDNNVRPAASDLVKYGEYMATVADCATCHTPFVKGQLD
                     FSRMYAGGNTFHIPKTVVNSANLTSDSATGIGTWTEERFLNKFTAYREEKGYKYDAGN
                     QNTVMPLVDYAGMTDADL"
     assembly_gap    982..992
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<993..1536)
                     /locus_tag="PK977_00940"
     CDS             complement(<993..1536)
                     /locus_tag="PK977_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF16692.1"
                     /translation="MKKYIAFAAMFTLIIAAAYFSSCKDKKSEPTAGDNKEDSLRKVI
                     ERGDYLANHVAACIHCHSKRDFSQYSGPVTPGTEGGGGDVFSNEILDAIPGVFYGKNI
                     TPDNETGIGTWTDDEILRAITQGISKKGDTLFPLMPYANFNRMAKDDLLAIIAYLRTL
                     KPIKNKVPERKMMIPIAMAYP"
     assembly_gap    1738..1756
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1757..2761)
                     /gene="nagA"
                     /locus_tag="PK977_00945"
     CDS             complement(<1757..2761)
                     /gene="nagA"
                     /locus_tag="PK977_00945"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRF16693.1"
                     /translation="MRVLNASRIFTGDRWLTDHSVIIEDGKFKELISNTELPSSFEPE
                     RYEDCIITPAFIDLQIYGASGRLLAVYPETNSIESLVYYCRTGGAAWCLPTVATNTKE
                     VFFKSIDAIRDYLSKDGKGVLGIHLEGPWINKVKRGAHIESLIHSPSVNEVKDLLEHG
                     KGIIKMITLAPECVNKEVIDLILSYDVVVSAGHSNASYTEAMKGFANGVQLVTHLYNA
                     MSPLHHREPGLVGATMDHKRVMASIIPDGYHVDYAAIRIAKKAMGERLFAITDAVTDT
                     NEGYYQHQLAGDKYEAGGVLSGSALTMQKALQNLVKECGIDLGEALRMCSLYPARVLR
                     L"
     gene            complement(2794..3345)
                     /locus_tag="PK977_00950"
     CDS             complement(2794..3345)
                     /locus_tag="PK977_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16694.1"
                     /translation="MRKGLIIILFMTSHLAGAQHCPWDCSGMILLKTDATSGEMSRLN
                     PVLVDGDRKLVIDTLYGTGLNSTADTCQFLVYEDFLKFRVAKTAIHYWYSYDTVYHFA
                     KGHYLVKYNYCKYNRNNESLYIRINDLNNKPSGYRYIEVTAEKGIHLHNYHRLLNDRK
                     TGELLTALAPMVIFISRVDLGLN"
     gene            3453..4787
                     /locus_tag="PK977_00955"
     CDS             3453..4787
                     /locus_tag="PK977_00955"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF16695.1"
                     /translation="MAGSFLGRIFTCSVFFICLGISPSLSGQQREKVLHDLNSLLINT
                     VMDDLFNPTVSSRIYTYPNIAFYECIRNEDKSYPSLTGKLTAFPKLTVLPKPKTDYLI
                     AAAISFSYVGQSLVGSEHKIEAWRNQFVDSLRLTVDTSILSNSIAFGKTTADQIIAWV
                     KKDNYGETRAMMRYVISDKDGKWQPTPVDFMPALEPNWGYMRPLTLKKASQFSPEKKL
                     VFSMEKNSPFYKNVLEVYNTVKNLDSTKKAIALYWDDNPNISVEQGHFNYFIHKISPA
                     GHWLMITRQACLEKNISLVKASRAYTLTSIALYDAFISCWYEKYKTDLVRPITVIKRY
                     IDEKWEPYIQTPPFPEFTSGHAVISNAAATVLTRLLGENYSFTDNTEIPFGIQPRTFA
                     SFYKAAEESAWSRVYAGIHYPETARISIKQGKQIGQYVLNTILPSQGTKTSK"
     gene            4784..8146
                     /locus_tag="PK977_00960"
     CDS             4784..8146
                     /locus_tag="PK977_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF16696.1"
                     /translation="MSAIRIFVFLLCCYAYALPGATQSPGEKKLFTALPSSYTGVTFR
                     NDIHEDNKFFYYDYMYLYNGAGISIGDINNDGLQDLYFSSTTGFNKLFLNLGNLQFKD
                     ITEQAGVNGGMGIKTGVNMIDLNTDGLLDIVVSKSGPFDIQYRQKIVYINNGNLTFTD
                     RAAEYGLNEPGHTTQTYFLDFDRDGDMDAFFVNHSMDFNNNMVVNAKIENGKMVMIDD
                     TARQYVSCRLYENRNGHFTDISQKAGISTNSFALSASIADINNDGWPDIYVANDFKKP
                     DYLFINNKNGTFSDKLTDYFKHISQASMGSDIVDINNDGLEDVFVLDMAFEDPVRQKK
                     LFTQHLNYDKFHLGVQLGLFHQYPHNVLQLNNGNGTFSEIAYHAGVAETDWSWTPLIA
                     DFDNDGWKDIYVSNGLKRDVNDWDYRGFFIDSIKNLIAKGIKVDMVQWFSQIPSVPIK
                     NYFFRNNGSLRFDNYTDKWTDEPASISNGAAYADLDNDGDLDIVVNNVEGEAFVLKNN
                     QNEINRSGFIRFRLLKDKNNKQEVYGATVKLTLKNQKSQFQRYEPQRGYMSSNEHYLH
                     FGTGNEAFVEEVTVAFPSGKTQTLKNISSGQVLTLYEEDAAELSSNEKVSSPALFAPD
                     SERKRFSYTHKENDFIDFKREPLIPYKCSRKGPFYAKADVNGDGREDIYIGGAAGSEG
                     VLMLQNADGSLSKKNQASFVNDKKMEDGGAVFFDADGDMDLDLYVVSGGSEFPASSPL
                     YQDRLYINDGKGNFTRSLTALPRETNNGSVVVAADIDDDGDMDLFVGGGVMPGKFPRH
                     DKNIILQNNRGKFINITDTFAPELANTGIVNYAAWGDIDGDKKNELVIAGEWMPVMIF
                     KSSNNKLNQVTSYVTINSHSGKTENISMGQIKGWWNTILLTDIDKDGDLDILAGNRGT
                     NSRIVAKLEEPCTIYAKDFDNNGSYDAILGYYIRGKCYPMYHRDQLIDQMPSMRKKFN
                     RYHLYATKTLDEILSEEQKKGMDIYTTTCFESGIFINEGNNHFRFEEFPELAQISTIN
                     DMIADDFDNDGNMDIIIGGNSSDPEIGTGSYDAMAALFLKGDGKGKLTVNTLSGLPNN
                     GEIRRIINFTQNNSLILLKNNEPAQVILKKAN"
     gene            complement(8149..8838)
                     /locus_tag="PK977_00965"
     CDS             complement(8149..8838)
                     /locus_tag="PK977_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF16697.1"
                     /translation="MTNVKVLYVEDELFLGKIVRESLECRGFEVIMEDDGAKVLDLFK
                     KTNPDICVLDVMLPNKDGFAIADEIRELNEDVPIIFLTAKTQTEDVVKGFTLGGNDYI
                     RKPFSMEELIVRIQNLLRNKSEGTTKISGGATAIGKYTFHLNRQVLAGEKEERKLSYR
                     ESELLKLLYENRDKIIDRKDILNLLWGNDSFFNSRNLDVYITKLRSYLKEDTSLEIIT
                     IKGIGYRFVTG"
     gene            complement(8835..>9857)
                     /locus_tag="PK977_00970"
     CDS             complement(8835..>9857)
                     /locus_tag="PK977_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF16698.1"
                     /translation="VFNLLYGVDSLQDSLKVAEVDSAYRRALAEEKINIPFSITRADT
                     SSTEASTLNEVTIGLANPVTYKLELGNTFPYLMKRITQPILFSILLLGITILSFFLLY
                     RNLLKQRRLAELKNEFISNITHELKTPIATVGVAIEALRNFNAMQDPQRTKEYLDISS
                     NELQRLSLLVDKVLKLSMFEKKEIDLKYELVNMKEITDEVVSSLKLQLEKKNAAVSVT
                     AKGETGLQGDRLHLLSVVFNLLDNALKYSKGKPVIDIRIMGTDQQVIFSVTDNGIGIP
                     PAYTDKIFDKFFRVPHGDTHNAKGYGLGLSYAARVILQHKGTIDVYNNEGEGVTFTIH
                     LPKQIS"
     assembly_gap    9858..9867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9868..10314)
                     /locus_tag="PK977_00975"
     CDS             complement(<9868..10314)
                     /locus_tag="PK977_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16699.1"
                     /translation="MNRLRWLAILMIVAIIGITGFQLYWLRQTYEREKKSLNIKTEMA
                     FREAVLRLQMAKLKLDGINWEGKSGKPTVRVFMANDGEEHLKVKVAPRQEIVSTINVI
                     KDKLKDSLKHVTKGRTIITLNNSTMSVTKDSQGLDRPPLPPEAPGRD"
     gene            10452..11258
                     /locus_tag="PK977_00980"
     CDS             10452..11258
                     /locus_tag="PK977_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRF16700.1"
                     /translation="MKKYLLTAFGILGLLALQAQTSQGKVIYDRTVKLQFSFAGMPGG
                     MEQQMPTSRTDKYELIFGNNQSLWKQAENDNTDDGGAFQTEGGAQIRMIVAGSNDVLY
                     TNLESGKKTERRELMDKNFIVDDTIAKLKWKMTGETKTILGHNCMKATATRTSKRTIM
                     NMDNGVMERKEVDDTAAIVAWFATDIPVSAGPSEFQGQLPGLILELDVKDGTQLFKAV
                     SISEKADLAEIKEPTGKKRYTAAEFKEEREKMLKEMNQNNGGGRRVIRMN"
     gene            11372..12127
                     /locus_tag="PK977_00985"
     CDS             11372..12127
                     /locus_tag="PK977_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HRF16701.1"
                     /translation="MAAEIVSNDPNVVGLAVAGSWITNELDEYSDLDLILVTKEKVSN
                     DKEKMIAYAKTIGNIISGFTGEHVGEPRLLICLYDDPLLHVDIKFLTLEEFRNRIENP
                     VVLFERDNLLTNIIQSTRAEWPQPDFQWIEDRFWTWVHYACMKIGRGELMEAYDFLSF
                     LRQTVFSPLMQVKNNKKVRGLRRVETQLNLSDLENLKITIAQYNKASIIKALDNSISI
                     YKSLRRKLFPATITLQEKAEKRSLEYFKQIKKA"
     gene            complement(12138..14957)
                     /locus_tag="PK977_00990"
     CDS             complement(12138..14957)
                     /locus_tag="PK977_00990"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF16702.1"
                     /translation="MRKAVLFTFFLIVAATLFAQKNGIVKGIAFDTISRQPVAAATVT
                     VLEKKDSSLVTFTMTGSDGRFELRGLANGEYRLLITHVNYHNSNKYFTISDSSRLVDM
                     GNLPISDKTKVLEEVVLAAEAPPITMINDTIQYNTGSFKVQPNANVEDLLKRLPGVKV
                     EKDGTIKAQGEKVNRVLVDGKEFFGNDPKIATKNLPADAVDKVQVYDRQSDQAQLTGF
                     EDGNYEKTINLKLKKDKKKGLFGKAMAGAGTKERYESRFNLNSFKGARQLSAIGMGNN
                     TNAEGFSFMDILNFTGELARMQRAGGGNININLSADDAAAMGVNTGGRNSGINTTWGG
                     GLNYNNIIGNKLDFQSNYFYNRFNPRQESSIERQYFLPDSSYFYRQNALSDNLSNNHR
                     LNLNLLYQVDSMNSLRITPSFSYQKATDRSQSDYQTLSEDKILANAGFSNTTSNNDGY
                     TFRNEAIWRRKFARKGRTFSLSLQTSLNESDGDGSLSSINSFYNPNGSLLRKDTLNQQ
                     SLSESDLRGYTARAVYTEPVFKKSLLELSTSRSNTKSTSQRKTYDFNKGNGKYDQLNN
                     MLSNNFENTYGFTNGGLRIRTQKKKYNYAVGVTWQQAELEGKIISGIKDSTISKTFHN
                     WLPNARFQYNFTRFKNFTLSYNTSTNQPTMSQLQPVPDISNPLNIRDGNPDLKQEFMH
                     TVQGNLTLVSPYKNKNLFLFFTLQSTQNKIVNYDTVNQLGVKRTKPVNVNGVYNLNSN
                     ISYSLPVRFLKGTIELSSRTGYFKSKQFINGSGNDIKTLSFGPELRLDMNPTEKLNVA
                     IGASVSYNNTKYSLRPELNTKNISQEYNASTDWQLPKQFFFSTNFTYTINSQRAAGFN
                     LEVPIWNASISKQFLKYNRGELKLSAVDLLNKNIGVSRNTNNNYIEDSRVLTLRRFFQ
                     LGFTYSLSKTGLNNAGGGGMRIIAR"
     gene            complement(15138..>15392)
                     /locus_tag="PK977_00995"
     CDS             complement(15138..>15392)
                     /locus_tag="PK977_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16703.1"
                     /translation="FNQLNNLSVRFADNKSLHLKGKFEEPKEEMKFTSVLFSPEPGQE
                     FLKKLKVEITMQAVEPMEWKVKLLIYDREREDEERGAVRE"
     assembly_gap    15393..15420
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15421..15739)
                     /locus_tag="PK977_01000"
     CDS             complement(<15421..15739)
                     /locus_tag="PK977_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16704.1"
                     /translation="MKKTVKPLVLLTICALFFIQTPAQLKMPVAAGISSDIKKIIEDY
                     PNRFINLMGEVIMQHPQSTDYECNFKVNGAEESFITRYSAKKEICSFEAVMMTTENFD
                     KAKQ"
     assembly_gap    15777..15799
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15869..16021
                     /locus_tag="PK977_01005"
     CDS             15869..16021
                     /locus_tag="PK977_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16705.1"
                     /translation="MKNRHLIHHHEAAKIYSNYYKPSFNLLRLLKSVLSVLISGSSPA
                     AGHYRR"
     gene            complement(16101..>18503)
                     /locus_tag="PK977_01010"
     CDS             complement(16101..>18503)
                     /locus_tag="PK977_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF16706.1"
                     /translation="NDPKLATKNLTADMVESVQVFDDMSEQARFTKVDDGSRTKAINI
                     KLKKDKNKGVFGRALAAGGNSSENGGRYEGNLSLNAFNGSNRVSLLFNANNINKQGFS
                     FSDIISSMGGFSGFNNSGGGGRGSFGGGGGGSFGGMNVSGGRGSFMGLTGTGPTGITR
                     SLSSGLNFNTEGAKIKASGSYFYSNTRNEQEQNTYRQTFFPDDSIAYLTRRSVSDNKN
                     QNHRFNVRMEYMIDSMNSILYTPSLTIQKSDNFNDDTSSTMSTSPNLNYLALTSRTTN
                     TNVRDGLNLGNNFLYRKKFKKIGRTLTLGWNNTYGESESEGLNISPIRFYKPDGSQQG
                     NNNQNQRNEQQTYTRNNVVSVSYTEPAGLNKLFEFNYAYTNNRNTSDKKTFNYNTVTG
                     DYDIKNLLLTNSFNNIFEASRFGANFRVQEKKYNYQVGLGVQRATLTSESYQAITNKD
                     SLTKQSFTNFFPQASFNWTPSRSKGIRFNYRGRTNQPSVTQLQDVPDVSNPLFIRTGN
                     PALKQEFSHSVNLNYNTFNILTFKFLAANLNFSTTQNKIVNSIDTVFSGASNPNVPQG
                     QKGIQYSRPVNLNGAFTLSSFFTVGLPFKSVKLKGSSLNFTTLALFNRDVSELYKQAN
                     IGKTLTLTQTAGANFNLKEKWDLSANASLSYYNVKYSVNESLNEDYFAQTYSADVART
                     FTKPGIILSTDIDYYVNSGRAEGFNQSIPLWNASIAKQLFKKKNGELKFTVNDILNQN
                     QSITRNTGDNYFEDVNSMVLRRYFMVSFLFNLNRMGGNNQQQQPGMPRFMERNMRNMR
                     MY"
     assembly_gap    18504..18513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18514..>18891)
                     /locus_tag="PK977_01015"
     CDS             complement(<18514..>18891)
                     /locus_tag="PK977_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF16707.1"
                     /translation="SNKQGVFEIKGLTEGNYQLVISYQGYETVRKPVSLTATSKSVDI
                     GEINPPKDFKTLGEVVVTGIVPVVIKQDTVQFNADAFKTRPNATVEDLLKKIPGVQVD
                     KDGNVQAQGEQVQKVYVDGKEFF"
     assembly_gap    18892..18957
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19181..22945)
                     /locus_tag="PK977_01020"
     CDS             complement(19181..22945)
                     /locus_tag="PK977_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRF16708.1"
                     /translation="MALPHLIKYVYTHGTDEVIRRGKKIHAIGFVELVEYDDLFGSAV
                     FRVKDDSYSTYYKVYIQKFKDPKATSLRCACPYNLGDICRHEAAALFQLQEMIDRGHL
                     QSEHVQYDQRHTVVKMKTIDLKNLRLFSSPEVFAEAEKFLRTNKAAIEYAENEMVKAA
                     VALNGQSYQVIIRKNEERNFDTSCDYEDSEHPLCLPKVIVLLQLLNTYGANYFDSIRN
                     WDKEKNKLLEAYGYSLSDDLTGKFEFAYKEGKPFLRVLDPAIKRVAPVAAPVKPVLLP
                     QKEKEEVIVPVKEEVKPVLPSQKLGVVFNFNKKAYPGFTVDVVIGEPNDSNDGFAGTA
                     EKLDISRYVSTDNLSETDGELLTMLRKLQETEINKYVSRNSPFSGIWENIIHQEEDDL
                     PDETKELIAEYLLPKLRKIFTELAGSPFVFLLPERKAFKTNNLEPLQLQAEPARPHFI
                     VKKNSHYNIQCKVKTGVLEFELGDNETASPLFFLYNQQLYLWQSNDVIHLVDKFLPSG
                     KMSVPGEEWGKCLTQFILPLAKEHKVDFDKSLVQEVKDGNPEVKLFLLEKGEYLVFQP
                     SFTYKGYETKSRDRDEVIVPQGDKVMVVHRNREKENEFIQKLQNLHSSFAYNEDTGTL
                     ALKGTDVLKNNWFFLFVDAMNEMKTPVFGFEALKNFRFNTAKPQTKIFISSNTDWFDA
                     KVDIVFGDQKVTVAEVKRALANKQQFVQLNDGTLGILPEEWLKKYSLLFRVGEGKTDS
                     LKLSRYHLSVVDELYETRDEEELVVKLEEKYENLRQFNKINEIAPSDHLQPILRPYQV
                     AGYQWLNYLKDINWGGILADDMGLGKTVQALSFMEHYRNEQGKLKALVVCPTTLIYNW
                     ENEIRKFTPGLTYRIHHGGLRTRVKEELVDHDVTITTYGTLRSDIKLLMSVEFDYVIL
                     DESQAIKNPSSKVTKAACLLNAKHRLCMSGTPLQNNTFDIFAQMNFLNPGMLGTVEFF
                     RQEFAIPIDKFGEQDRKEHLKKLLYPFILRRTKEQVAKDLPEKQEMILFCEMEEEQRN
                     IYDAYRNDFRNQILGTIETQGIQKSQLTILQGLMKLRQICDSPAILNEQEKFENHSIK
                     LDELAREITENIGDHKALVFSQFLGMLGLIRKKLEELGIKYEYFDGSTSAPDREKAIQ
                     SFQNDHEVRVFLISLKAGGVGLNLTAADYVYIVDPWWNPAVEQQAIDRTHRIGQTKNI
                     FAYRMICKDTIEDKILQLQEKKRALAKDIISDDATFVKTLTREDVEYLFS"
     gene            complement(23102..23827)
                     /locus_tag="PK977_01025"
     CDS             complement(23102..23827)
                     /locus_tag="PK977_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRF16709.1"
                     /translation="MSNKPSLHTSISPALLHLYDLSNSVVVIIDVFRATSTIASALYN
                     GAKCVIPVDTVPKAIEISKNIGGIAAGERDGMIAEGLQHGNSPLEYTREFIENKTLVL
                     TTTNGTRLLQMALDKNADTIISGSFPNLSAVCDYLLSEKKNVVLGCAGWKDRFNLEDT
                     LFAGAVIHQLSKHFTVHCDSSLMAETMYTKYKNKLIDFAPKLTHYHRLVERFGLIEDI
                     RFCLTEDVANVLPVYSNGKLIAK"
     gene            complement(23873..24349)
                     /locus_tag="PK977_01030"
     CDS             complement(23873..24349)
                     /locus_tag="PK977_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF16710.1"
                     /translation="MPTIHLTTFIAAPAEVVFDLSRHIGLHKESMSHYKEEAVAGTRM
                     GLIEKEETVTWRAQHLFKNRLLRVKITEMKKPELFVDEQLQGDFKMMKHEHHFKPCEN
                     GTLLIDLFHFETPYGIVGRWLNALYLTNYMKKLLEQRNRTIKEVAESDRWKRLLIK"
     gene            complement(24419..>24605)
                     /locus_tag="PK977_01035"
     CDS             complement(24419..>24605)
                     /locus_tag="PK977_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRF16711.1"
                     /translation="EIKDHSGNTIGQVTSGTQSPTLGKAIGMGYVQTAFAAIDSEIYI
                     QVRDKSLKAKVAKIPFS"
     assembly_gap    24606..24615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24616..25518)
                     /gene="gcvT"
                     /locus_tag="PK977_01040"
     CDS             complement(<24616..25518)
                     /gene="gcvT"
                     /locus_tag="PK977_01040"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRF16712.1"
                     /translation="METRNTPFTSKHIALGAKMAPFAGYNMPISYTGINDEHAAVRKN
                     AGVFDVSHMGEFILKGENALDLIQRVTSNDAAKLKAGQAQYSCLPNENGGIVDDLLVY
                     CIEENKVYMLVVNASNIEKDWNWISKHNTNNVEMHNISEKTCLLAIQGPNATKILQPL
                     TEMDILNLKYYTFTKGVFAGVENVLVSATGYTGAGGVEIYFEDKDGAADKIWDAIFEI
                     GGPQGLKPIGLGARDTLRLEMGFCLYGNDIDDTTSPMEAGLGWITKFTKEFTSKEIFA
                     KQKAEGVQRKLVGFEMIDKGIPRHG"
     gene            complement(25655..27835)
                     /locus_tag="PK977_01045"
     CDS             complement(25655..27835)
                     /locus_tag="PK977_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRF16713.1"
                     /translation="MKKIININLSGRVIPIEDSAYEKLQVYIESLRRYFANEEGRDEI
                     INDIESRIAELMNEKIRKGAAAVTDQDIEEIITAMGRVEDFEAEDKETTEAAQPGTQP
                     NFTYTEKKSKRLYRDTNDKFIGGVCSGLAAYMNVDPAIVRILFAIISFGGFGFGFLAY
                     IILWIVLPPKDLEGYIGKRLYRNPDDKVIGGVAGGLAAYFNKSASTIRLIFAAPLLLS
                     ILVGILNGFRWHYDVDFALNLGFGSLSGTFILTYIILWIVLPEANSDYQKMEMRGETV
                     DVNRIRQNVREGMENMKEKMKGWGEEVKESAQSFSNKAKEFANTRGKEFASEVNEPVR
                     RGGGGIGHAFGVLFKVFFLFIAGTIAFALFVTLIALLFGGVAWWPINNFLWTSKWQQI
                     FAWGTLIFFLLVPLIGFITWVVRRIIRAKSKNNYLGWTFGGLWTIGWVSVTFFVASII
                     KDFREFEHTDIPVTITQPAGGKMIVAVSQPELEYTGSFGWMNDDSEGWDMTKDTLKIG
                     AVKFSVKASQDSLYHVNIRKHSFGRTESEALSRASKIDFDAMWRDTVLDVANGYAIDK
                     DSKFRGQHVEIEILVPVGKQIRFDESVQRKLNAINIRVKRSYRRDRITGIEIDNDERA
                     FRFRTGIDYVMGLDGELKGPDGMVDQKPAKDEYRYDSSEKANDSLELEKSIEQKKKEL
                     QELEERKNKQKVKSTTSIKNKRRSNEDGSYASGPNPASSLVEWF"
     gene            complement(27865..28215)
                     /locus_tag="PK977_01050"
     CDS             complement(27865..28215)
                     /locus_tag="PK977_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRF16714.1"
                     /translation="MNIENTQSQMRKGILEFCILSIIRRGEAYPSDIVEEMRAANLQI
                     LEGTLYPLLTRLKNADMLTYRWVESNSGPPRKYFSLTEKGETFYKELEQTWNELYNGV
                     NALTSKKPGETPTN"
     gene            28455..29492
                     /locus_tag="PK977_01055"
     CDS             28455..29492
                     /locus_tag="PK977_01055"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HRF16715.1"
                     /translation="MLNYRYLIALYICTSLLYSCSNKYKAFQSRYKFSSDDGSPAYQH
                     LNYWAAHPWKHDPSDSVPAPLRNERRDTLVDVFFLHPTTFTKKKYKHIPNATINDHYI
                     NAKTDYSSILYQASVFNQSCRVFAPRYRQAHIQNFYTSDTAMAKKAFDLAYEDIRNAF
                     QYYLQHWNNERPIIIAGHSQGALMAERLLKEFFEGKKLEKKLVAAYIIGWPVPQDYFT
                     TLPMCKDSTQTGCVISWRTFRKGFVPSYLKKSKEIVLATNPLIWSTDKRYAPKQLNKG
                     SVLTKFNKVYERTTDGQLANGLLYVKKPKFPWSFLYFTSNYHIADINLFYLNIRENVR
                     QRVSVMIKKPY"
     gene            complement(29486..31096)
                     /locus_tag="PK977_01060"
     CDS             complement(29486..31096)
                     /locus_tag="PK977_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16716.1"
                     /translation="MIGGSSIKYSYFTLFVIVASLVLVFTTIFTPLLNSGDDAFFMYT
                     LAGGFGEEPTNLLHYNQGWHPWLGGLLSTLFTKWPGVNWYSITLLVFHCCGLTALLYA
                     MLKTLKPGIGILFFLAIFLFIESRFLLSLNFTGTAFVTSTGAMSLMIYQVNKQKVFCV
                     LNVSAFLLLLLSGMLRLHVVILVMLLFIPFIISSLSKKAKLSIFKAYLLSGIVLLLLN
                     KAQENFYIDNIPGWKKQESFRQALFYAYNRPKNPDVFKIAFKDSTEQELFFSSFLYDS
                     VQFTPVRITEISSKITRKRGLNNKEDILGLYWFFMELRIYFLLIITFVITFFFSKQIG
                     QIHRWLIPMITVLFVYAYLFVFLKITTTIHLGLLFVLLVQISVGFQGVAPVFPFKWIR
                     LFMGSVFILLVAWMLARLNKENDINKERRRNFNCVLAELNQNKDKLFIATDDSFPLGH
                     FYLWDTPLQFPVSNLVYKDRMLTHTYLKTLKRYGVNDLHKALRENTNIRLVGKKLLSL
                     EKILDGVRISQRMPDFKCMEVRKLERIQ"
     assembly_gap    31206..31228
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31229..32562)
                     /locus_tag="PK977_01065"
     CDS             complement(<31229..32562)
                     /locus_tag="PK977_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16717.1"
                     /translation="MVSFFLFLVYFIYRIILIFYQHPDAGGVEGNIIYFIQRLLDGLP
                     IYTNPERAPYAIAQYSPLYYYLVAGASKLIGLTSDDILSIYMVSRSISLFLNIIFIFI
                     IYLTANRAFSVSKEKSISVAIAAFIFLEITSFSRPDSLNHVFFFLTLYVFMNGIKTGK
                     PVKPTIIFTAMLAAVSIYCKQTSFLLPVIIGIWLLYRKMIIELFLFSFFYLVLTMVFI
                     LPVYINGELGVFYSNVVQGINNGISLTWFRSVILSDYYFSFGLLFVPTCIIVLYQLKK
                     ETNELLRFSYFLMIVLFVLLNIVSLKNGSNPGYFTEWWSLLFILLAYIGPQLTKAVSA
                     IDKRIPAGIILFVFIIKAIEIEWPLRKMLTSISDSQSFKKDKEVADYILGKLPSDSRH
                     TVFTNFNTADSYLSNILNKQVVAPQMDIIGLSTYAYKKFDYSDLIKSFKDGR"
     gene            32925..33752
                     /locus_tag="PK977_01070"
     CDS             32925..33752
                     /locus_tag="PK977_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF16718.1"
                     /translation="MKTIIIPTDFSPTATNAMHYGIDMAKAIHASVILLHVYQVPVSY
                     TDTPIVLVSVEELRKEAEQKLEKLHKEVEHLTSGSIKIYTEARLGNIADEIENLCEKV
                     KPFAVVMGSKGSTGIEKILFGSTTLTAIRHLTWPVICVPPGKVYGNGIKKIGFACDFR
                     NIVETTPANAIKTFVKEFGAELHILNVDYHNKQFKPETPEQSALLHTMLEEVNPLYDF
                     IEHRDIEDGLNEFAEKNNLDLLITIPKKHKLLDGLFKPSSTKQLVVQSHIPVMCIHE"
     gene            33812..34222
                     /locus_tag="PK977_01075"
     CDS             33812..34222
                     /locus_tag="PK977_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16719.1"
                     /translation="MKCKLNAGVLFLSAMLSLYACGGKNKKADDKKETTTETTVKDSG
                     GDDGIVPNIDTASLKDEASILDAMQKVADARLADEKKKQEDPNYSGHYLELTKIYTAV
                     LKASTAYSKTLTDPNKAIEFNNKVSEIQKKMYAK"
     assembly_gap    34273..34364
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34365..34642)
                     /locus_tag="PK977_01080"
     CDS             complement(<34365..34642)
                     /locus_tag="PK977_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16720.1"
                     /translation="MDLAMTAEGPPTDLAKSAALTVLTKGNSIYYYQGEWEEAVLNLK
                     VEPVELSGKNSLRAVIGRMQISLDKNENLINGRDELMILIKPGPGTSY"
BASE COUNT        10038 a         7265 c         6569 g        10453 t
ORIGIN      
        1 ccggttgaaa aaataaatag tttaaaccag gtatgtatga aaaagcaaat tgtattatcg
       61 ctattgtcag taatcctgtt tgcatcatgt ggcaataaca aagcagatca gccaactgaa
      121 acaaatgatt catccactat tttatatagc tgggaagcta ccctcaatga ttctaccgga
      181 agactggaag ttgtaaaaag ggaaggtttg tcccctgatt ctttgtcggt acaagcagtt
      241 attgattttc taaacaggaa aaaccagttt gtacaaattc aatatagcaa aacctcggga
      301 gacactgtat ttgtcgggat acccgaagcc acttatctaa cccagcagat gggttcatcg
      361 gggccaactg ttttttttgc ggaggctgtt tacaatctga ctgaaattcc gggcatccgt
      421 tttgtgaact ttgattttga agaaggcgac catgcttcac cgggtgtttt tgacagaaat
      481 acatttaagg atcaataaaa aaccccggta tatgccaggg ttttttattg atagtcaact
      541 atcttattta ggaaacttct ctaccttatt annnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnntc aggtcagcat cagtcatgcc ggcatagtcc accagcggca
      661 tcacagtgtt ttgattacct gcatcatact tgtatccttt ttcttctctg taagcagtaa
      721 atttattcaa aaacctttct tcagtccatg tgccaatacc cgtagctgaa tcgctggtga
      781 gattggctga gttcacaact gttttaggta tatggaaagt attaccacct gcatacatcc
      841 ggctgaagtc aagttgtccc tttacaaaag gtgtatgaca agtagcacag tctgccactg
      901 tagccatgta ttcgccatat ttcacaagat cagatgctgc gggtcgaaca ttattatcaa
      961 ctgaaggttg taaagcctgt gnnnnnnnnn nnccgggtaa gccatggcaa taggtatcat
     1021 cattttcctc tctggcactt tattcttgat tggctttaaa gtacggaggt aggcgattat
     1081 agccagcagg tcgtctttgg ccatcctgtt gaaattggca tagggcatca acggaaataa
     1141 ggtgtcgcct ttcttactaa tgccctgagt aatggctctt aaaatttcat catctgtcca
     1201 tgttccgatg ccggtttcat tatccggtgt tatgttcttt ccataaaaaa caccgggaat
     1261 agcatccagt atttcgttac tgaaaacatc tcctcccccg ccttctgttc cgggggtgac
     1321 ggggcctgag tactggctaa agtctctttt actatgacag tgaatacagg ccgccacatg
     1381 attagcaagg taatctcccc tttcgattac ttttctcagg gagtcttctt tgttgtctcc
     1441 ggcagtgggc tccgattttt tgtccttaca ggaagaaaaa taagcagcag caatgatgag
     1501 agtgaacatt gctgcaaagg caatgtactt tttcatgttg gttttgttta agttggttgg
     1561 caaaaaagca ataaaaggta taaacaattt ttcaatgcct ctaaaaaaaa tttggaagac
     1621 ttctggcaga ataaagcaga aacttttgtt gatagaagtt aagcagttat tagctgcttc
     1681 agctgtaatg tattatccaa tactacaaga tcggctttaa accctttttc aattttgnnn
     1741 nnnnnnnnnn nnnnnnaagc cgtagtaccc gggcaggata aaggctgcac atccgaagag
     1801 cttctcccaa atcaatccca cattccttca caaggttttg caaagccttt tgcatggtca
     1861 aagctgaacc actcagcact ccgccagcct catacttgtc tccggccagc tgatgctggt
     1921 aatagccctc atttgtatca gtcactgcat cagtaatagc aaacaatctt tcacccattg
     1981 cttttttggc aattcgaatg gcagcatagt caacatgata gccatcagga atgatacttg
     2041 ccatcaccct cttatgatcc atggtggcac ccacaagtcc cggctcccgg tggtgcaacg
     2101 gactcattgc attatataaa tgtgtaacaa gctgaactcc gttagcaaag cccttcatgg
     2161 cttcggtata gcttgcgttg ctgtgtccgg cagaaacaac aacatcatac gagagaatca
     2221 ggtcaattac ttccttgtta acacactctg gagcaagggt gatcattttg ataattccct
     2281 ttccatgctc cagaagatct tttacttcgt ttacagaagg cgaatgaatc agcgattcaa
     2341 tatgtgcccc tcttttcact ttattgatcc atgggccttc gaggtgaata ccgagtactc
     2401 ctttgccatc tttgctcaaa taatctctga tcgcatcaat gctcttaaaa aaaacttctt
     2461 tcgtattagt tgctactgtt ggcaggcacc aggctgctcc gccggtacga cagtaataaa
     2521 ccaggctttc gattgaattt gtttccggat aaactgccag caatcttccg gatgcaccgt
     2581 atatctgcaa atcaataaat gccggggtaa taatgcaatc ttcatagcgt tccggctcaa
     2641 aggaagaagg taattctgta tttgaaatca gttctttaaa ctttccgtct tcaataataa
     2701 cagaatgatc tgttagccat ctgtcgcctg taaaaattcg actggcattt aatactctca
     2761 cggtttacgt atttttaagg aagataaagc aatctaattt aacccaaggt ctacccgact
     2821 aataaaaata accattggtg caagagctgt aagcagctca cctgttttcc tgtcattaag
     2881 taaacggtga taattatgca agtgaattcc tttttcggca gtcacttcga tgtatctgta
     2941 ccctgaaggc ttattgttta aatcgtttat acggatgtat aatgattcat tgtttctatt
     3001 atacttacaa taattatact tgaccaggta atggcctttt gcgaaatgat agactgtgtc
     3061 ataactatac cagtaatgaa tagctgtttt ggcaacccgg aattttaaaa aatcttcgta
     3121 aaccaagaat tggcaggtat cggctgtaga gtttaacccc gttccgtaaa gagtgtctat
     3181 aactaatttc ctgtcaccat caaccagtac cgggttcagc cggctcattt caccggatgt
     3241 tgcatcggtt tttaataaaa tcattccact gcaatcccag ggacaatgtt gtgccccggc
     3301 cagatgcgag gtcatgaaca ggataattat gagtcccttt ctcatagtat taaaataagt
     3361 atttaaaata taatcgttct acccaactcc gggtatttca tcttcttcgc cctcaataaa
     3421 aagctgtaaa ttcatatcaa taatcaatta tcatggctgg atccttttta ggcaggattt
     3481 ttacctgctc tgtctttttt atttgccttg gaatttcacc ttcgctaagt ggtcaacaga
     3541 gagaaaaagt gctgcatgac ctgaatagtc tattaataaa tacggtaatg gatgaccttt
     3601 tcaatcccac tgtctccagc cggatatata cttacccgaa cattgcattt tatgaatgta
     3661 ttcgtaacga agacaaatct tatccctcat tgaccggtaa gttaacggca tttcctaaac
     3721 tcacagtact acccaaacct aagacagact atttgatcgc tgctgcaatt tcattttcct
     3781 atgttggcca aagccttgtt ggttctgagc ataaaataga agcgtggcgc aatcagtttg
     3841 tcgattcctt aagactaaca gttgacacca gcatactttc caactccatc gcttttggta
     3901 aaacgacagc tgatcaaatc atagcatggg taaaaaaaga taactatggt gaaacaaggg
     3961 caatgatgag atatgttata tctgataaag atggcaaatg gcagccaacc ccggtggatt
     4021 ttatgcctgc attggaaccc aactggggct atatgcgtcc attgacattg aaaaaggctt
     4081 cccagttttc acctgaaaaa aagcttgttt tcagcatgga aaaaaattca cctttttata
     4141 aaaatgtgct ggaagtatat aacacagtta aaaatctcga cagcactaaa aaagcaattg
     4201 ccttatactg ggatgacaac ccgaatattt ctgttgagca gggtcatttc aattatttca
     4261 ttcacaaaat atcacctgca ggacattggt tgatgataac ccgccaggct tgcctggaaa
     4321 aaaatatttc acttgtcaag gcgtcccgtg cctatacact gacttctatt gctttatatg
     4381 atgccttcat cagctgctgg tatgaaaaat ataaaactga tctggtaagg cctatcactg
     4441 taataaaaag atacatcgat gaaaaatggg agccatatat acagacacct ccttttcctg
     4501 aatttaccag cgggcatgca gtcatttcaa atgctgcagc aactgtatta acccggctgc
     4561 tgggagaaaa ttattctttc acagacaata ctgaaatacc ctttggtatt caaccccgga
     4621 cctttgcttc tttttataaa gcagcagaag aaagtgcctg gagccgtgta tatgccggta
     4681 tccactaccc ggaaacagcc aggataagca ttaaacaggg aaaacaaata gggcaatacg
     4741 tattaaatac catattacct tcacagggaa caaaaaccag taaatgagtg ccatcaggat
     4801 ttttgtcttt ctgctatgct gctatgcata tgcattaccg ggagctacac agtcacctgg
     4861 cgaaaagaaa ttgtttacag cgctaccttc ctcttatacc ggtgtaacct tccggaacga
     4921 tattcatgag gacaataagt ttttctatta cgattatatg tatctctata atggtgcagg
     4981 tataagtatt ggtgatatta ataatgacgg gttgcaggac ctgtacttta gttccacaac
     5041 aggctttaat aagctttttc tcaatcttgg caaccttcag tttaaagaca ttactgaaca
     5101 ggccggggta aacggtggca tgggtataaa gaccggtgta aatatgattg accttaatac
     5161 cgatggcctg cttgatatcg tagtttcgaa aagcggcccc tttgatattc agtaccggca
     5221 aaagatcgta tacatcaata acggtaatct aacatttact gacagagcag cagaatatgg
     5281 gctgaatgag cccggtcata ctacacagac ttatttcctg gattttgaca gggatggtga
     5341 tatggatgct ttttttgtaa accactcaat ggacttcaac aacaacatgg tagtaaatgc
     5401 aaaaatagag aatggcaaaa tggtgatgat tgatgatact gccaggcagt atgttagctg
     5461 caggttatat gaaaacagga atggccattt taccgacatt agtcaaaagg cgggtatttc
     5521 caccaactcc tttgccctta gcgcctctat tgctgatatc aacaacgatg gctggcctga
     5581 tatttatgta gcaaatgatt ttaagaaacc ggactatcta tttatcaaca acaaaaacgg
     5641 tacgttctct gataaactga ccgactactt taagcatatc agccaggcat ctatgggctc
     5701 cgacatcgtt gatatcaaca atgatgggct tgaggatgtt tttgtactgg atatggcctt
     5761 tgaagacccg gtaagacaaa agaaattatt tacacaacac cttaactacg acaagtttca
     5821 cctgggagtg cagttggggc tttttcatca atacccgcat aacgtcttgc agcttaataa
     5881 cggcaatgga actttcagtg agatcgctta tcatgcaggg gtagcggaaa cagactggag
     5941 ctggactcct cttattgctg attttgataa tgatggctgg aaagatatat atgttagcaa
     6001 tggcttaaaa cgggatgtaa acgactggga ttacaggggc ttttttattg attcaattaa
     6061 aaatttaatt gcgaagggca tcaaagtcga catggtacaa tggtttagcc agataccctc
     6121 tgttcccatt aaaaattatt tcttccgcaa taatggttca ctacgctttg acaattacac
     6181 tgacaaatgg actgatgaac ccgcatctat ttctaatggc gctgcttatg cagaccttga
     6241 taacgacggt gatctcgata ttgttgtgaa caatgtggaa ggagaggcat ttgttttgaa
     6301 aaacaatcag aacgaaatta accgatcagg ttttatccgt tttcgtttat taaaagataa
     6361 gaataataag caggaggttt acggagcaac tgtaaagctc actttaaaaa atcaaaaaag
     6421 ccagtttcag cgatatgagc cacagcgggg ttatatgtct tccaacgagc attaccttca
     6481 tttcggaaca gggaatgaag catttgttga agaggttacg gtcgcttttc catccggaaa
     6541 aacacaaact ttaaaaaata tcagttccgg acaggttctt accctatatg aagaagatgc
     6601 tgcagaactc agtagcaatg agaaagtatc ttctccagca ctgtttgccc cggatagcga
     6661 aagaaaacga tttagctata ctcacaaaga aaatgacttt atcgacttta aaagagagcc
     6721 gcttatcccc tacaagtgca gccgcaaagg ccccttttat gccaaagcag atgtaaatgg
     6781 tgatggccgt gaagatattt acattggtgg cgctgccggt agtgaaggag tgttaatgct
     6841 tcaaaatgca gatggaagtt tatccaaaaa aaatcaggct tcttttgtta acgacaagaa
     6901 aatggaagat ggcggtgctg tattttttga tgcagacgga gacatggatt tggatttata
     6961 tgtcgtcagc ggaggttcag agtttccggc ttccagcccg ttataccagg acaggctcta
     7021 cataaatgac gggaaaggaa attttacccg ttcactgacg gcattaccca gggaaacaaa
     7081 taatggttct gttgttgttg ctgctgacat agacgacgac ggggatatgg atctttttgt
     7141 tggcggtggc gtaatgccag gcaagtttcc caggcatgat aaaaatataa tactgcaaaa
     7201 caacagaggt aagttcataa atattacaga cacctttgca cccgaactgg ctaatacagg
     7261 aattgtaaat tatgcggcat ggggagatat tgatggtgat aagaaaaatg aattggtaat
     7321 agccggagag tggatgccgg taatgatctt taaaagcagc aataacaagc taaaccaggt
     7381 tacttcatac gtaacgataa acagtcacag cggcaaaaca gaaaatatat caatgggcca
     7441 aatcaaagga tggtggaata caattttgct gaccgatatt gataaggacg gagacctgga
     7501 tatactggcc ggtaaccgtg gtaccaactc aagaatagtg gccaaactgg aggaaccctg
     7561 taccatatac gccaaagact ttgacaataa cggaagttac gatgctatcc tgggttacta
     7621 tatcagggga aaatgttatc ccatgtatca ccgagatcag ctgatcgatc aaatgccttc
     7681 tatgcgcaaa aaattcaacc ggtatcatct ttatgcaact aaaacactag atgaaatttt
     7741 gagtgaagaa caaaagaagg gaatggatat ttacacaaca acctgctttg aatcaggcat
     7801 ttttattaat gaaggaaata accattttcg gtttgaagaa tttcctgagc tggcacaaat
     7861 atctacgatc aatgatatga ttgcagacga tttcgataat gacggaaata tggatattat
     7921 tattggcggc aactcatccg atccggaaat cggaaccgga agctatgatg ctatggctgc
     7981 cttattcctg aaaggagatg gtaaaggaaa attgacggtc aatactttat ctggcttgcc
     8041 caacaatggg gagatcagaa gaataatcaa cttcactcaa aacaattcgc tcatattatt
     8101 gaaaaataat gaaccggctc aagtcatttt aaagaaagca aactaacctc atccggtaac
     8161 aaaccgatag ccaatccctt tgattgtaat aatttctaaa gaagtatctt ctttcaggta
     8221 gctgcgtaat ttggtaatgt aaacatcaag gttgcggctg ttaaaaaaag aatcattgcc
     8281 ccaaagcagg ttgaggatat ctttccggtc aatgatctta tcccggttct catataacaa
     8341 tttcagcaat tcgctttcac ggtaagacag tttacgttcc tctttctccc ctgctaatac
     8401 ctgcctgttc aggtgaaaag tgtatttacc aattgctgta gcccccccgc taatttttgt
     8461 agtgccttcc gacttatttc gcaaaaggtt ttgtatccgg acaataagct cttccatact
     8521 gaagggcttt cgtatataat cgttaccgcc aagtgtaaat ccctttacca catcttctgt
     8581 ctgtgttttg gccgttaaaa atatgatggg aacatcctca ttcaattctc ttatttcatc
     8641 agcgatggca aaaccatcct tattgggcaa catcacatcc aggacacaga tatcaggatt
     8701 tgttttcttg aacaagtcaa gcactttggc cccgtcatct tccatgatca cttcaaagcc
     8761 gcggcattcc aggctttccc tgactatctt acccaggaaa agttcatcct ccacatataa
     8821 tacttttacg tttgtcacga tatttgtttt ggtaaatgaa tggtaaatgt gaccccttcc
     8881 ccttcgttat tataaacatc tatagttcct ttatgctgca gaatgaccct tgcagcataa
     8941 cttagaccca atccatatcc ctttgcatta tgcgtatctc catgaggtac ccggaaaaac
     9001 ttatcaaata ttttgtctgt atacgctggt ggaatgccaa ttccgttatc tgttacgcta
     9061 aatataacct gctgatctgt tcccataatc cggatatcga tcaccggttt tcctttactg
     9121 tacttcaggg cattatccag caggttgaaa acaacgctta acaaatgaag cctgtctccc
     9181 tgcaaacctg tttcgccttt cgcagttaca ctcactgctg cattcttctt ttccagctga
     9241 agttttaagg aagaaaccac ttcgtctgtt atctccttca tattaaccag ctcatacttc
     9301 aggtcaatct ctttcttttc aaacatggat aacttcagta ctttatctac cagcaaactc
     9361 agtcgctgta actcattgga agaaatatcc aggtattctt tggtacgttg gggatcctgc
     9421 atcgcattaa aattccgtaa tgcctcaata gctacaccaa ctgttgcaat gggcgtcttt
     9481 aattcatgag tgatattgct gataaattcg tttttcaatt ctgccagtct tctttgtttt
     9541 agcaagttac ggtatagcaa aaagaaggac agtattgtaa tccccaataa tagtattgaa
     9601 aataatatcg gttgggtaat ccgtttcatc aggtagggaa atgtgttacc cagttctagc
     9661 ttataagtaa cagggttggc cagcccgatt gttacctcat ttaacgttga cgcttctgtc
     9721 gaagaagtat ctgctctggt tatcgaaaag ggaatattta ttttttcttc agccaatgcc
     9781 cgccggtaag cagagtcgac ttccgctacc ttcaacgaat cttgtaaaga atccaccccg
     9841 tataatagat tgaacacnnn nnnnnnnatc cctgcccggg gcttctggcg gtagtggggg
     9901 tcggtcaaga ccttgagagt cctttgttac actcattgtg ctattattga gtgtaataat
     9961 tgtacgacct tttgttacat gtttaagcga atccttgagc ttgtctttta ttacattgat
    10021 agtagatact atttcctggc ggggggcaac tttaactttc aagtgctctt cgccgtcatt
    10081 tgccataaat accctcaccg taggcttgcc gctcttgcct tcccaattta tgccgtctag
    10141 cttcaatttg gccatctgta accgcagtac cgcttcccgg aaagccatct ctgttttaat
    10201 attcagtgat tttttctccc gttcataggt ttgccggagc caatacaact gaaagccggt
    10261 gatgccgata atggcaacaa tcattaatat ggctaaccag cgaagacggt tcatacaatt
    10321 tgtttacaga acaaatgtaa atcaatactg gcggtagttt cagggccatt aacctttatt
    10381 aacctaaaag aaaggtggct taacttatct tgcctgtttc catatatagt tttgttgaaa
    10441 acatctaaac aatgaagaaa tatctcctta ctgcttttgg catacttggt ttattggcat
    10501 tacaagcaca gaccagccag ggaaaagtaa tctatgaccg cactgtaaaa ctgcagttca
    10561 gctttgccgg tatgcccggt ggtatggaac aacagatgcc taccagccgt actgataagt
    10621 atgaactaat cttcggcaat aaccaaagcc tatggaaaca ggctgaaaat gataatacgg
    10681 acgatggggg tgcctttcaa actgaagggg gagcacagat aagaatgatc gtagccggga
    10741 gtaatgatgt actctatacc aacctcgaat ccggtaagaa aacagaacgc agggagttaa
    10801 tggataaaaa ttttatcgtg gatgatacaa tcgccaaact aaagtggaaa atgaccggtg
    10861 aaacaaaaac aatccttggt cataactgta tgaaggcaac tgcaacccgt accagcaaaa
    10921 gaacgataat gaacatggat aatggtgtaa tggaaagaaa agaagtagat gatacggctg
    10981 caattgttgc ctggtttgct accgatattc ccgtttctgc cggaccttca gaatttcaag
    11041 gacaattacc tggcttgata ttggagttgg atgtaaaaga tggtacacaa ctatttaaag
    11101 ccgtgagtat ttcagaaaaa gcagatttag ctgaaattaa agaaccaacc ggtaaaaaaa
    11161 gatatactgc tgccgaattc aaagaagaaa gagaaaaaat gctgaaggaa atgaaccaga
    11221 ataatggcgg aggaagaaga gttatccgca tgaactgata atattaatgc tttcaaaaat
    11281 aaccgttctg cacctgcagt gcggtttttt attgcccaag gccattgcat tatttatctt
    11341 tagttaaatg atacagaaag attttgcata tatggcagcg gagatagtga gtaatgatcc
    11401 gaatgtagtg gggctggctg ttgcaggttc atggatcacc aatgaactgg atgagtattc
    11461 tgaccttgat ctgattttgg taaccaaaga aaaagtcagc aatgacaaag aaaaaatgat
    11521 agcctatgcc aaaaccattg gtaatatcat ttccggcttt accggggaac atgtagggga
    11581 gccacggttg ttgatctgcc tctatgatga ccccttactg catgtagata tcaagtttct
    11641 tacgctggaa gaatttcgaa acagaattga gaaccctgtt gtactttttg aaagggataa
    11701 tcttcttacc aatatcatac agtctaccag ggctgagtgg ccacaacctg attttcagtg
    11761 gatcgaagac cggttctgga catgggtgca ttatgcctgt atgaaaatcg gccggggtga
    11821 actgatggaa gcttatgact ttctctcttt tttgcgccaa acggtattct ctcccctgat
    11881 gcaggtaaaa aacaacaaaa aggttcgtgg ccttagaaga gtggaaacac aactcaatct
    11941 ctccgacctg gagaatttga aaataaccat tgcacagtat aacaaagcat ccattatcaa
    12001 agcactggac aactcgatca gtatttacaa atcacttcgc agaaaattat ttcctgcaac
    12061 tattaccctg caggaaaaag cagaaaagag aagccttgaa tattttaaac aaattaagaa
    12121 ggcctaaaga aaatgttcta tctcgcaata attctcatac cgccgccacc ggcattattc
    12181 aaacctgttt tactaaggct gtaagtgaag ccaagctgga agaaacggcg tagtgttaat
    12241 acccggctgt cttcaatgta gttattgttt gtattacggc ttacaccaat atttttattc
    12301 aagaggtcca cagcgctgag cttaagctct ccgcggttat acttcaggaa ttgtttgctg
    12361 atacttgcat tccagattgg tacttcaagg ttgaaccctg ctgcccgctg gctgttgatg
    12421 gtataagtga agttggtaga aaagaaaaac tgtttaggca attgccagtc tgttgatgcg
    12481 ttgtactcct gtgatatgtt ttttgtattc agctcaggcc gaagggagta tttggtatta
    12541 ttgtaactga ccgatgcccc aatcgctaca tttaattttt cagttggatt catgtctaac
    12601 cgaagctccg gaccaaatga cagtgtttta atatcattac cacttccgtt aataaactgc
    12661 ttgcttttaa aatacccggt gcggctgctc agttcaatag ttcccttgag aaaacgaacc
    12721 gggaggctat aactgatatt gctgttaaga ttgtatacgc cgtttacatt cacaggtttt
    12781 gttcttttta cgcccagctg attgacagta tcgtaattca caattttatt ctgcgtagac
    12841 tgcagggtga aaaagagaaa caggttttta tttttatacg gactgacaag tgtaagattc
    12901 ccctgcaccg tatgcatgaa ctcctgtttc aggtcagggt taccatctct tatattcagc
    12961 gggttgctga tatcaggaac aggttgcagc tgtgacatgg ttggctgatt ggtagaagtg
    13021 ttgtaactga gtgtaaagtt tttaaacctc gtaaaattgt attgaaacct tgcattgggc
    13081 aaccagttgt gaaatgtctt gctgatagtt gaatccttga taccgctgat gatctttcct
    13141 tccagctctg cttgctgcca ggttactccc accgcatagt tgtacttctt cttttgtgtg
    13201 cggatacgaa gtccgccatt ggtaaagcca taggtatttt cgaaattatt actcagcata
    13261 ttatttaact gatcatactt tccgtttcct ttattgaaat cataggtctt acgctgcgat
    13321 gtactttttg tattgctgcg gcttgtactc aattccagca atgatttctt gaaaacaggc
    13381 tcggtatata ctgccctggc tgtataaccc ctcaaatcac tttcagaaag actttgctgg
    13441 ttcagcgtat cctttctcaa taaagagccg ttgggattat aaaaactgtt gatggaggaa
    13501 aggcttccat cgccgtcact ttcattcagg cttgtttgca ggctgagtga aaatgttctc
    13561 cccttgcggg caaattttct cctccagata gcttcatttc gaaatgtata gccatcatta
    13621 ttgctggttg tgttgctaaa tcccgcatta gcaagaattt tatcttccga taatgtctgg
    13681 taatcagatt gtgagcggtc agtggctttt tggtagctga aagatggtgt aatacgtaat
    13741 gagttcatcg aatccacctg gtacagcagg ttcaggttca accggtgatt attgctcagg
    13801 ttatcagaca gtgcattttg ccggtagaag taagaagaat caggcaaaaa atattgtctt
    13861 tctatgctgc tctcctgcct gggattaaac cggttataga aataattgct ttgaaaatcg
    13921 agtttgttcc caataatgtt attgtaattc agtccgcctc cccaggtggt attgataccg
    13981 ctattccgtc cgcctgtatt tacacccata gctgctgcat catctgccga caggttgata
    14041 ttgatattac cgcccccggc cctttgcatc cttgccagtt caccagtgaa gttcagaata
    14101 tccataaagg aaaatccttc tgcattggta ttattgccca taccaatagc cgatagttgt
    14161 cttgctcctt taaaagaatt taaattgaac ctgctttcat agcgttcttt ggtgccggca
    14221 cctgccattg cttttccaaa caaacctttt ttcttgtctt tttttagttt gaggtttatc
    14281 gttttttcgt agttaccatc ttcaaaacct gttagctgtg cctggtcgct ctgcctgtca
    14341 tacacctgca ccttgtccac tgcatcggcc ggcaaattct tggtggcaat cttgggatcg
    14401 tttccaaaga actctttccc atctaccagt accctgttca ctttttcgcc ctgtgctttg
    14461 attgttccat ctttctctac tttcacccca ggtagtcttt tcagcaggtc ttccacatta
    14521 gcattgggtt gtactttaaa agatcctgta ttatactgta tagtatcatt gatcatagtg
    14581 atgggcggcg cctccgctgc cagtactact tcttcgagca ctttggtctt atcgctgatg
    14641 ggcaggttgc ccatgtcaac cagcctgctg ctatcactga tggtaaaata tttattgctg
    14701 ttatggtagt tcacatgagt tatcagcaga cgatattccc cgttggcaag tcctctcaat
    14761 tcaaagcggc catcgcttcc tgtcatggta aaagtaacca gtgaagaatc ttttttttca
    14821 agaactgtta cagtcgctgc agccacgggt tgccggctga tcgtatcaaa agcaatccct
    14881 tttacaatac catttttctg ggcaaataat gttgccgcaa ctatcaaaaa gaaggtaaat
    14941 aagacagctt ttctcatagc gaagttattt cagctacgaa aatattcctg gtgatctatt
    15001 taatgggtta accggtcttg ttttaatgtt aatgaaggtt aaagaatgag tcttaagtcc
    15061 gaagtcaata gtccatagtt atttaaaact gcatagtctt gactctatat taaagacaat
    15121 ccgctcccgg ctaagtttta ttctctgacg gctcctcttt cttcatcctc tctttcacgg
    15181 tcgtagataa gcaattttac tttccactcc atcggttcca ccgcttgcat ggtgatctca
    15241 actttcaatt tcttcaaaaa ctcctgtccg ggttcaggtg agaataaaac agaagtaaac
    15301 ttcatttctt cttttggttc ttcgaatttt cctttcaggt ggagagattt gttatcagca
    15361 aaccggacag aaagattatt gagctggttg aannnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 tttgctttgc cttatcaaaa ttctctgtgg tcatcatcac tgcttcaaag ctgcatattt
    15481 cttttttggc agagtaacgg gtaatgaacg attcttcagc cccattcact ttaaaattgc
    15541 attcatagtc tgttgactga ggatgttgca taatcacttc acccatcagg ttaataaacc
    15601 ggttaggata atcttcgata atttttttga tatcgctgct gatacctgct gctaccggca
    15661 tctttaactg tgccggtgtt tggataaaga atagtgcgca aatagtaagc agcacaagtg
    15721 gttttacggt ttttttcatc aatgttggat ttaaccggtt agtaacaggc tccaatnnnn
    15781 nnnnnnnnnn nnnnnnnnna gaaaaattta tttttattac atacttttaa agtgaaacta
    15841 ttcgtttacc cttaataaaa tatccattat gaaaaacagg catctgatac accaccacga
    15901 agcggctaag atctacagca attattacaa accttcattt aatttattgc gattgctgaa
    15961 gtctgtgctg tctgtattga tctcaggttc atctcctgct gccggtcatt acagaagata
    16021 aacatgcagt actaacccat cattatactg acaagggcct cactcatgtg aagcccttgt
    16081 tttattttag caggatttgt ctaatacatt ctcatattcc tcatatttct ttccatgaag
    16141 cggggcatcc cgggttgttg ctgttgatta ttcccaccca tacgattcag gttaaacagg
    16201 aagcttacca tgaaataacg cctcagcacc atactgttca catcttcaaa gtagttgtcg
    16261 ccggtgttcc gggtaatgct ctgattctgg ttcagaatgt cattcaccgt aaactttagt
    16321 tcaccattct ttttcttgaa aagctgtttg gcaatggagg cattccataa aggaatactc
    16381 tggttaaagc cttctgccct tccggagttt acatagtagt caatatcggt agaaagaata
    16441 atcccgggct tggtgaatgt gcgggccaca tcggctgaat aggtttgtgc gaaatagtct
    16501 tcattcaatg attcgtttac tgaatacttc acattgtagt aagaaaggct ggcgttggca
    16561 gaaagatccc atttttcttt caggttgaaa ttagcacccg ccgtttgtgt caaggtaagc
    16621 gttttaccga tattggcctg cttgtacaat tcacttacat ccctgttgaa taatgccagt
    16681 gtagtaaagt tcaggctgga tcctttcagt ttcacacttt tgaatggcaa tcccactgta
    16741 aagaaggatg acaacgtaaa tgcaccattc aggttaaccg gccggctgta ttgaatccct
    16801 ttttgtccct gcggtacatt aggattagaa gctcctgaaa aaacagtatc aatactgttg
    16861 acaatcttgt tttgagtagt gctgaaattc agattagcag caaggaattt aaaagtaaga
    16921 atattgaaag tgttgtaatt caggttaacg ctatgactga attcctgttt caatgccggg
    16981 ttacccgttc ttatgaataa aggattagaa acatccggca catcctgtaa ttgtgttaca
    17041 ctgggctggt tggtacggcc ccgataatta aatcgtatgc ccttgctgcg gcttggtgtc
    17101 cagttaaagc tggcctgcgg aaagaagttg gtgaaggatt gttttgtaag cgaatccttg
    17161 ttggtgatag cctggtagct ttcgcttgtc agtgtggctc tttgcacacc cagaccaacc
    17221 tggtaattat attttttctc ctgtaccctg aagttggccc caaaacgact ggcttcaaat
    17281 atattattga aactattggt caacagaagg tttttaatat cataatcacc cgtaaccgta
    17341 ttatagttaa atgtcttctt atctgatgtg tttcggttat tggtgtatgc ataattgaac
    17401 tcaaacaatt tattcaaacc ggcaggttct gtatatgaaa cactcactac attattgcgg
    17461 gtataggttt gttgttcatt gcgctggttc tggttattat taccctgctg tgatccatcc
    17521 ggcttataaa aacggatagg ggagatattt aatccctcgc tttcgctttc accataggta
    17581 ttgttccagc ccaatgtaag tgtgcggccg atctttttaa atttcttcct gtacaggaaa
    17641 ttattaccaa ggtttaaacc atctctcaca ttggtattcg tggtacggct ggtcaaagcc
    17701 aggtaattca aattgggcga agtggacatg gttgacgatg tatcatcgtt gaagttgtca
    17761 gacttttgaa tagtaagtga cggagtgtaa agaatagagt tcatggagtc aatcatgtat
    17821 tccattctta cattaaaacg gtgattctgg ttcttattat cggatacaga acgacgggtg
    17881 aggtaagcaa tagaatcatc ggggaagaaa gtctgacgat aagtgttctg ctcttgttca
    17941 ttcctggtat tagaatagaa ataacttccg ctggctttta tttttgctcc ttctgtatta
    18001 aagttcaacc cgcttgacaa agaacgggtg atgcctgtag gaccagtgcc ggtaagtccc
    18061 ataaagctgc cccggccacc ggatacgttc attcctccaa aacttccacc gcctccacca
    18121 ccaaatgagc ctcttccacc accaccacta ttattgaaac cgctgaaccc acccatagag
    18181 ctgatgatat cagaaaagga aaagccttgc ttattaatat tattggcgtt gaaaagaaga
    18241 gacaccctat tgctgccgtt aaatgcattc aggcttaaat taccttcata ccggccgcca
    18301 ttttcactgc tatttccacc agctgccaat gctcttccaa agactccttt attcttatcc
    18361 tttttcagct tgatgttgat ggccttggta cggctgccat catctacttt ggtaaatctt
    18421 gcctgttcgc tcatgtcatc aaacacctgt acactttcta ccatatcagc agttaaattc
    18481 tttgtagcga gtttgggatc gttnnnnnnn nnncaaagaa ttcttttcca tccacataca
    18541 ctttttgtac ctgttcgccc tgtgcctgta catttccgtc tttatctacc tgtactcccg
    18601 gaattttctt cagcagatct tccactgttg catttggcct tgttttaaat gcatctgcat
    18661 tgaactgtac tgtatcctgc tttatcacta ccgggacgat acctgtgacc actacttctc
    18721 ccagcgtttt aaagtctttg ggcgggttta tttcacctat atctaccgat ttggatgtcg
    18781 ccgtaaggga aaccggtttt cggacggtct catatccctg gtaagaaatc accaactggt
    18841 aattcccttc ggtcagacct ttaatttcaa atacaccttg cttattggat annnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt
    18961 ttaccagcag tatcaatcag ctttccttta atggatccat ctgccttttg ggcatcggca
    19021 ttgagtaaga ccccaaataa cagaagggat aaggtaaaaa tcttcttcat aatcagttta
    19081 ataagtgtac gaaaatagac gtaagaacaa cctgcaacct gcgaaggttg ggtgtttttg
    19141 agaaaaaaag gatggatagt tcgattaccc gatagacggt ttaactaaac aggtattcca
    19201 catcttccct cgtcagcgtt tttacaaacg tagcatcgtc ggaaatgatg tccttggcca
    19261 atgctctttt cttttcctgc aattgcagaa ttttatcttc gatggtatcc ttacagatca
    19321 tccggtaggc aaaaatgttt ttagtctggc cgatacggtg tgtacggtca atggcttgtt
    19381 gctccacagc agggttccac cagggatcaa cgatatatac ataatcagca gcagtaagat
    19441 tcaaacccac accaccagcc ttgagcgaaa tgaggaatac cctcacttca tgatcattct
    19501 ggaaactctg aatggccttt tccctgtctg gtgcagaggt gcttccgtcg aagtattcgt
    19561 atttgatacc caattcttcc agctttttac gaatcaatcc aagcatgccc aggaactggg
    19621 aaaacaccag cgccttatgg tcgccgatat tttcggtgat ctctcttgcc agctcatcca
    19681 gcttgataga atgattttca aatttctcct gttcattcag gatcgcaggt gaatcacata
    19741 tctgccgcag tttcatcaat ccctgcagaa tggtcaattg tgatttctga ataccctgtg
    19801 tttcgatagt acccaatatc tggttccgaa aatcattgcg gtacgcatca taaatatttc
    19861 gctgctcttc ttccatttca cagaaaagga tcatctcctg tttttctggc agatctttgg
    19921 ccacttgttc ttttgttctt cttaatatga aaggataaag cagttttttc aggtgctctt
    19981 ttcggtcctg ttcgccaaac ttatcaattg gtatagcaaa ctcctgccgg aaaaattcta
    20041 ccgtgcccag catgccgggg ttcaaaaagt tcatctgcgc aaaaatgtca aatgtattat
    20101 tctgcaaagg cgtaccgctc atgcaaagcc ggtgtttggc gttcagcaaa caagcggctt
    20161 tggttacttt acttgatggg tttttgatgg cctgcgattc atccagtatc acatagtcaa
    20221 actccacact catcagtaat ttgatatcgc tgcgcaatgt gccatacgta gtaatagtca
    20281 catcatgatc gaccagttct tctttcaccc ttgttcttaa tccgccatga tgtatgcggt
    20341 acgtaagacc cggagtaaat ttcctgatct cattctccca gttataaata agagtggtag
    20401 gacaaaccac cagggctttc agttttccct gctcatttcg gtaatgctcc ataaaggaaa
    20461 gggcctgtac tgttttaccc aaacccatat catctgcgag gataccaccc cagttaatat
    20521 ccttaaggta attcagccat tggtaacctg caacctggta aggacggaga atcggctgaa
    20581 gatgatcaga tggagctatt tcatttattt tattaaactg tctgagattt tcatacttct
    20641 cttcgagttt taccaccagc tcttcttcat ctcttgtttc atacaattca tccactacac
    20701 ttaaatgata acgggatagc ttcaatgagt ctgtctttcc ctcacccacc ctgaagagca
    20761 gggaatactt tttgagccac tcttccggca gtatgccgag agtgccatca tttaactgta
    20821 caaactgctg cttattggcc aatgcccttt ttacttcagc aaccgttact ttctgatcac
    20881 cgaaaacaat atccactttt gcatcaaacc agtcggtatt gctgctgata aaaattttgg
    20941 tttgcggctt ggctgtattg aaacggaaat tcttcagcgc ttcaaaacca aagacaggcg
    21001 tcttcatctc attcatcgca tctacgaaca agaagaacca gttattcttc agcacatcag
    21061 tacctttcag tgcaagggtt ccggtatctt cattataggc aaaacttgaa tgcaggtttt
    21121 gaagcttctg tatgaattca ttttctttct cccggtttcg atgcaccacc attactttat
    21181 caccctgcgg tacaataact tcatctctgt cccttgattt tgtttcatag cccttgtagg
    21241 taaaggaggg ttgaaaaacg aggtactcgc ctttttccag cagaaacagt tttacttcag
    21301 gatttccatc ttttacttcc tgcaccagcg atttatcaaa atccacctta tgttcctttg
    21361 ccagcggtaa aatgaactgg gtaaggcact tgccccactc ttctccgggc acactcattt
    21421 taccggatgg aaggaattta tctaccaggt gtatcacatc attactttgc cacaggtata
    21481 attgctggtt gtaaagaaag aaaagcggac tggccgtttc attatctccc agttcaaact
    21541 ccagtacacc cgtctttact ttgcattgaa tattataatg agagttcttt ttaacgatga
    21601 aatgcggtct tgctggttct gcctgcaatt gtaacggctc caggttattg gttttaaaag
    21661 ccttcctttc gggcaggaga aatacaaacg ggctgcccgc cagctcagta aatatcttcc
    21721 gcagcttggg caacaaatat tcggcaatca gctccttggt ttcatccggc agatcatctt
    21781 cttcttgatg aataatgttt tcccaaatac cagagaaagg tgagttcctg cttacatact
    21841 tgttaatctc cgtttcctgc aacttacgca gcatggtgag taattcgccg tcggtttcag
    21901 agaggttgtc ggtacttaca taccggctta tgtccagctt ttctgctgtt cctgcaaaac
    21961 catcattgct atcattgggt tcaccgatca ccacatctac cgtaaaaccc ggataggcct
    22021 ttttattaaa gttgaaaaca acacccagtt tctgtgaagg caacaccggt tttacttctt
    22081 cttttaccgg aacaattact tcttcctttt ctttttgtgg cagcaatacc ggtttcacag
    22141 gtgcggctac aggggctact cttttaatag cggggtccaa caccctcaaa aacggcttgc
    22201 cttctttata cgcaaattca aatttacctg tcaaatcatc actcagcgaa taaccatagg
    22261 cttccagcag tttgtttttc tctttatccc agttacgtat actatcaaaa taattggcgc
    22321 cgtaagtatt cagaagctgt agcaagacaa tcaccttggg aaggcacaag ggatgttctg
    22381 aatcctcata atcgcaactt gtatcaaaat ttctttcctc gttcttgcgg atgatgacct
    22441 ggtaggattg cccgttcaat gcaacagcgg cttttaccat ttcattttct gcatactcta
    22501 tagctgcctt gtttgtacgc aaaaattttt ccgcttcagc aaatacttcc ggagatgaaa
    22561 acaaacgcag gttcttcaaa tcaattgttt tcatttttac tacggtgtgc cgctggtcat
    22621 actgtacatg ctctgactgc aggtggcccc ggtcaatcat ttcctgtaac tggaagagtg
    22681 cagcagcttc atgacgacaa atgtcgccaa ggttgtacgg acaggcacaa cgcaatgatg
    22741 tcgccttagg atctttaaac ttttgaatat atactttata gtaagtagaa taactgtcgt
    22801 ccttgaccct gaacacagct gaaccaaaga ggtcatcata ttctaccagt tctacaaacc
    22861 cgatagcatg tatcttttta cctctgcgga ttacttcatc cgttccatgg gtataaacgt
    22921 acttgataag gtgcggtaaa gccataataa aaaatcacaa ataacaaaat gcaaattcca
    22981 aaaaggacaa tcggcaaaag taaaagaatg taaggcggat ttccttgcaa taagggagaa
    23041 ataattaata attcctgttt gtggtttata cggaaaaaat caagtacctt gtatgctttt
    23101 tttatttcgc aatcagcttg ccattactgt ataccggcaa tacattggca acatcttcag
    23161 tcaggcagaa ccggatatct tcgataagac caaatcgctc taccaggcga tgatagtgtg
    23221 taagcttcgg agcaaaatca atcagcttat ttttatactt tgtatacatc gtttctgcca
    23281 tcagcgaact gtcgcaatga acagtaaaat gtttggagag ttggtgaatc actgcaccgg
    23341 caaataatgt gtcctccagg ttaaagcggt ctttccagcc tgcacaaccc aacacaacat
    23401 tctttttttc tgataacaaa taatcacaaa ctgctgagag attaggaaaa gagccggaaa
    23461 taatagtatc ggcattttta tccagcgcca tttgcaatag ccgggtaccg ttggtagttg
    23521 tcagcaccag tgttttattt tctatgaact cccttgtgta ttccagcggt gaattaccat
    23581 gctgcagtcc ttcagcgatc atgccatctc tttcaccagc agcaatgccg ccaatattct
    23641 tactgatctc gattgcttta ggaaccgtat ccacaggtat cacacatttt gccccattat
    23701 ataatgcgga agcaatggta gaagtggccc tgaatacatc tataattact acaacactgt
    23761 tgctgagatc gtaaagatgc aataaagccg gagaaataga ggtatgcaga gaaggtttgt
    23821 tactcatatt gtgatgcaaa agtaacacat cagcaataaa caggattaag cactatttga
    23881 tcagtaatcg tttccatcgg tctgattcgg caacctcctt tatggttctg ttacgttgct
    23941 ccagcagttt tttcatgtag ttggtaagat ataatgcatt tagccagcgg cccacaattc
    24001 cgtaaggcgt ttcaaaatga aaaagatcga ttaacagggt tccgttttcg cagggcttaa
    24061 aatgatgttc atgtttcatc atcttaaaat cgccctgcag ctgctcatcc acaaatagct
    24121 ctggtttttt catttctgtg attttcaccc gaagcagcct gtttttaaaa aggtgttgtg
    24181 cccgccatgt caccgtttct tccttttcaa tgagccccat tcttgtccct gcaacagctt
    24241 cttctttgta atgagacata gattctttgt gcaacccaat atgacggctg aggtcaaaca
    24301 ccacttcagc aggtgcagct ataaatgtag taagatgaat ggttggcatc cgtgtaaata
    24361 taaataagaa gtgctgggtc tattctgatt acacaaaaat cctaccggcg attaattttt
    24421 atgaaaaagg aatcttggct actttagctt tcagggattt atctcttacc tggatgtaaa
    24481 tctccgagtc tatagccgca aaagcagttt gtacatatcc cattccaata gcttttccta
    24541 gagtgggtga ttgggtaccg gaagtaacct ggccaatcgt attgcctgag tgatccttta
    24601 tctcgnnnnn nnnnncccat ggcgggggat acctttatca atcatttcaa aacctaccag
    24661 tttccgttga actccctctg ccttttgttt ggcgaatatt tcttttgatg taaactcttt
    24721 ggtgaacttt gtaatccatc ctaatcctgc ttccataggt gatgtggtat catcaatatc
    24781 atttccatac aggcaaaaac ccatttcaag gcgaagtgta tctcttgccc cgaggccgat
    24841 tggttttaac ccttgtggcc cgccaatttc gaaaatggca tcccatatct tatcggcagc
    24901 accatcttta tcttcaaagt atatctccac tccccctgcc cctgtatatc ctgtagcact
    24961 gaccagtaca ttttccactc ctgcaaacac cccttttgtg aaagtgtagt atttcaggtt
    25021 aagaatatcc atttctgtca gcggttgcaa aatctttgtt gcattaggac cctgaatggc
    25081 cagtagacag gtcttttcag agatgttgtg catctccaca ttattggtat tatgttttga
    25141 aatccagttc cagtcttttt caatattgga agcattcact accagcatat aaaccttgtt
    25201 ctcttctata cagtaaacaa gcaagtcatc aacgatgccg ccattttcgt tgggcagaca
    25261 actgtattga gcctgtccgg cttttaattt agcagcatca ttgctggtaa ctctttgaat
    25321 gagatccaat gcattttctc ctttcaggat aaattcaccc atatgactca catcaaatac
    25381 gccggcattt tttcttacag cagcatgttc gtcattaatg ccggtgtagg aaataggcat
    25441 attataaccg gcaaatgggg ccatttttgc accgagagca atatgttttg aggtaaaggg
    25501 ggtattcctg gtttccatac agcaaatttt aagagtgagc aaaggtaagt gaatagtcaa
    25561 cgttcctaaa atcagaaagg cctctttcga agccttcctg taatcccatg gcgggatatt
    25621 caaccagtcc gcatccggac ttcaaccaaa ctttttaaaa ccattccacc aatgatgaag
    25681 ccgggttagg gccactggca taagagccat cctcattgct ccttctttta ttttttatac
    25741 tagtagttga tttgactttt tgcttgtttt tcctttcttc cagttcctga agttcttttt
    25801 tcttttgttc tattgatttt tccagttcaa ggctgtcatt agctttttcc gaactatcat
    25861 acctatactc atctttagcc ggcttttgat caaccattcc gtcaggccct ttcagctcac
    25921 catcaaggcc cattacataa tctatccccg tacggaaacg aaaagctctc tcatcattgt
    25981 caatttcaat cccggttatg cgatcacggc gataacttct tttcaccctg atgttgattg
    26041 cattaagttt tctctgaaca gattcatcga accgtatttg tttgcctacc ggtactaata
    26101 tttctatctc cacatgctga cctctgaact tactgtcctt atcaatcgca taaccattgg
    26161 ctacatccag tactgtatcc ctccacattg catcaaaatc gattttggat gcccggctta
    26221 atgcctctga ttccgttctg ccaaagctgt gtttcctgat attgacatga tacagagaat
    26281 cctggctggc tttaacactg aatttcacag caccgatctt taaggtgtct tttgtcatat
    26341 cccaaccttc actgtcatca ttcatccagc caaagctgcc ggtatattcc agttctggtt
    26401 gcgataccgc cactatcatc tttccgccag ccggttgcgt aatagtaacc ggaatatcgg
    26461 tatgttcaaa ttcacggaaa tcctttatta tacttgcaac aaagaaggta actgataccc
    26521 aacctatggt ccacaagcca ccaaatgtcc agcccaggta attattcttt gattttgccc
    26581 tgatgatacg acgtactacc caggtaataa agccgatcaa aggaaccagc aagaagaata
    26641 ttaatgtacc ccaggcaaat atttgttgcc atttactggt ccataagaaa ttattgatcg
    26701 gccaccaggc tacaccgcca aacaataatg cgatcagtgt aacaaacaat gcgaatgcaa
    26761 tggtgccggc aataaacagg aagaatactt taaacaatac tccaaaagca tgaccaatac
    26821 ctcctccgcc tcttcttacc ggttcattca cttctgaagc aaattccttt cctcttgtat
    26881 tggcaaattc tttcgcttta ttgctgaaac tttgtgctga ttcttttact tcttctcccc
    26941 atcctttcat tttctctttc atgttttcca ttccttctct tacattctgt ctaatcctgt
    27001 ttacatctac ggtttctcct ctcatctcca ttttctggta atcactatta gcttctggta
    27061 aaacaatcca taaaatgata taagtaagga taaaggttcc actcaaggag ccgaaaccca
    27121 ggttcaatgc aaagtcaaca tcataatgcc agcgaaaacc attgagaatt cctactaaaa
    27181 tgctcaacag gagaggggcg gcaaaaatca ggcggattgt ggaagcactc ttattaaaat
    27241 aagctgccaa accaccggct acaccaccaa ttactttgtc atcaggattt ctatacaatc
    27301 tcttgccaat atatccttcc aggtccttgg gcggcaatac aatccataat attatataag
    27361 caaggaaacc aaagccaaag ccgccgaaac taatgatggc aaagagtatc cttacaatag
    27421 caggatctac attcatatat gctgccagtc cgctacaaac accgcctata aacttatcat
    27481 tggtatcacg ataaagtctt ttgcttttct tttcagtata agtgaaattg ggttgtgtac
    27541 ctggctgggc agcttctgta gtttccttgt cctctgcttc aaaatcttcc actctgccca
    27601 tagcggtgat gatctcttct atatcctggt cggttactgc agcagcacct ttacgaattt
    27661 tttcattcat cagttctgct atgcggcttt caatatcatt gataatttca tcccggcctt
    27721 cttcattggc aaagtagcgg cgaaggcttt cgatataaac ctgcagcttt tcgtaagctg
    27781 aatcttcaat cgggataacc ctgccgctca ggtttatgtt tattatcttt ttcatgtttt
    27841 aattatttgg ttgaatcagg ttgattagtt ggttggtgtt tcaccgggct ttttgctggt
    27901 cagcgcattt acgccattgt atagctcatt ccaggtttgc tccagctctt tgtaaaaagt
    27961 ttcgcctttt tctgtcagcg aaaagtattt gcgggggggg cctgagttgc tttccaccca
    28021 gcgataagta agcatgtcag cattcttcaa ccgggttagc aatggataca gggttccctc
    28081 gagtatctgg agattggctg cccgcatttc ctccacaata tcactggggt atgcttcacc
    28141 ccggcgaatt atggagagta tacagaactc cagtatccct ttccgcatct ggctctgtgt
    28201 gttctcgata ttcatgataa tcgtcttttt taacttgaca agtcaaaggt agggtgatat
    28261 ttggtactat gcaaaacaaa gtaccatttt ttttagagta atgtagattg aatggaacac
    28321 aaataaaatt aaatgattga taattaatca ctttatataa attaatagtc acagatattt
    28381 agactgtcgg ttgtaattca tgatgagcga tctttgaacc ggatcaaaat cttgagcaac
    28441 ttaatcttac atcaatgcta aactaccgtt accttattgc cctttatata tgtacttctt
    28501 tgttgtattc atgttcgaat aaatataagg cctttcaaag ccgctataaa ttctccagtg
    28561 atgatggaag ccctgcgtac caacatttga attactgggc agctcatccc tggaaacatg
    28621 atccttcaga cagtgtgcct gctccattgc ggaatgaaag acgggatacc cttgttgatg
    28681 tgttttttct tcatccaacg actttcacaa aaaagaaata caagcatatt cccaatgcaa
    28741 caattaatga tcattatata aatgccaaaa cagattattc atccatactt taccaggcaa
    28801 gtgtgtttaa ccaatcctgt cgtgtatttg caccccgata ccggcaagca catattcaga
    28861 atttttatac cagcgataca gcaatggcca aaaaggcatt cgacctggcc tatgaggata
    28921 tccggaacgc ctttcaatac tatctacaac attggaataa tgaacggcca ataataattg
    28981 ccggacacag ccaaggagcc ctgatggctg aacgattatt aaaggaattc ttcgaaggaa
    29041 aaaaattaga aaaaaaattg gtggcagctt atatcatcgg ctggccagtt cctcaagatt
    29101 attttacgac tttacccatg tgcaaagatt caacacaaac aggttgtgtg atcagttgga
    29161 gaacatttcg caaaggtttt gttccttcat atctaaaaaa atcaaaagag attgtactgg
    29221 ccactaaccc actaatatgg agtactgata aacggtatgc accgaaacaa cttaacaaag
    29281 gcagtgtgct gaccaaattc aataaagtat acgaacgcac aaccgatggt caattggcaa
    29341 acggcctgct ttatgttaaa aaaccaaagt tcccctggag ctttttatat ttcaccagta
    29401 actatcatat tgcggatatc aacctttttt atttgaatat cagggagaat gttcgtcaaa
    29461 gagtaagtgt catgataaaa aagccctatt gaatccgttc aagttttctt acttccatac
    29521 acttaaaatc gggcattctt tgcgaaattc ttactccatc gagaattttt tcaaggctaa
    29581 gcaatttttt tcctacaagc cgtatattgg tgttttcccg taatgcctta tgcaaatcat
    29641 ttacaccata tcttttaagt gttttgagat acgtatgggt tagcattctg tctttatata
    29701 ctaagtttga aacagggaat tgaagaggag tgtcccataa ataaaagtgt ccaagaggaa
    29761 aactgtcatc agtagcaata aataatttgt ctttgttctg attcaactcc gccaagacac
    29821 aattaaaatt gcgacgacgc tctttattta tatcattctc tttattaagc ctggccagca
    29881 tccaagcaac aagtagtatg aagactgatc ccataaaaag acgaatccat ttaaaaggaa
    29941 aaaccggagc aacgccctga aaacctacgg aaatttgcac caataaaaca aataaaagtc
    30001 caagatgaat agtagttgtt attttaagaa aaacaaacaa ataggcgtag acaaataata
    30061 ctgtaatcat tggtattaac catcggtgta tttgacctat ttgcttacta aaaaaaaagg
    30121 ttatgacaaa agttataata agtaagaaat agattctcag ttccataaaa aaccaataca
    30181 aaccaagtat atcttcctta ttattcagcc ctcgtttccg tgttatttta ctgctaatct
    30241 cggttatcct cacaggagtg aattggacgg agtcatataa aaaagacgaa aagaataatt
    30301 cctgttctgt gctatcttta aaagcaattt tgaaaacatc tggatttttt ggccggttgt
    30361 aggcataaaa taaggcctgt ctaaagctct cctgtttctt ccagcccgga atgttatcaa
    30421 tatagaaatt ttcctgtgcc ttgtttaata acaataaaac gattcccgat aaaagatatg
    30481 ccttgaatat agaaagttta gccttttttg aaagagaact aataataaag ggtataaata
    30541 ataacatgac aaggataaca acatggaggc gcaacattcc cgataacagc aacaacaaaa
    30601 aagctgaaac atttaataca caaaatactt tttgcttatt aacttgataa atcattaacg
    30661 acatagcacc agtactagta acaaatgcgg tacccgtaaa atttagtgac aataaaaatc
    30721 ggctttctat aaaaaggaaa atcgcaagga aaaagagtat gcctataccc ggtttcaaag
    30781 tcttcagcat tgcataaagg agtgctgtca aaccacaaca atgaaaaaca agaagtgtta
    30841 ttgaatacca attaactcct ggccatttcg tgaacaaagt acttaataat ccgcccaacc
    30901 aaggatgcca gccctgattg tagtgcaaaa gattggtagg ttcttcccca aaacctccgg
    30961 ccagagtata catgaaaaag gcatcatcgc cggagttaag taacggtgta aaaattgttg
    31021 taaatactaa caccaatgat gcaactataa cgaataaggt aaaataagaa tactttattg
    31081 agctaccacc gatcataatc ttgttacggt ctatatttat aaatattaaa tccgttcata
    31141 gtcgtgtcaa gttcaaattt attcagatcg atatcaaaga attgcttttg cggccccaag
    31201 gctctnnnnn nnnnnnnnnn nnnnnnnnat acgaccatct ttgaaagatt ttatcagatc
    31261 gctatagtca aactttttat atgcataagt agataatcct ataatatcca tttgcggagc
    31321 tacaacctgc ttattaagaa tattgctcaa ataactatct gcagtattga agttggtgaa
    31381 aacagtatga cgactatctg aaggtaattt acctaaaata tagtccgcaa cttctttgtc
    31441 ttttttaaaa ctttgtgaat cacttattga cgttaacatt tttcgtaacg gccattcaat
    31501 ttctatagcc ttaattatga atacaaataa aattattccg gcaggtattc gtttatcaat
    31561 agctgataca gcttttgtta attgtggtcc tatgtaagca agcaaaatga ataacaaact
    31621 ccaccactcg gtaaaatacc ctggattaga accgtttttt agagatacga tgtttaacag
    31681 aacaaacaaa acaatcatca agaaatacga aaatcgcaat aattcgtttg tttccttttt
    31741 taattggtaa aggacaataa tgcatgttgg gacgaataat aacccgaaag aaaaataata
    31801 atcagaaaga attacactcc tgaaccaggt aagactgatt ccgttgttaa tcccctgaac
    31861 tacgttacta taaaacactc ccagttctcc gtttatataa accggaagaa tgaagaccat
    31921 tgtcaacacc aggtaaaaaa agctaaataa aaaaagctca ataatcattt ttctataaag
    31981 cagccaaatc ccaattatga ctggtaaaag aaaagaggtt tgcttacaat aaattgaaac
    32041 ggcagccagc atggccgtaa atattatagt tggcttaaca ggcttaccgg ttttaatacc
    32101 gttcatgaaa acatagaggg tgagaaaaaa gaatacatga ttaagactgt ctgggcggga
    32161 aaaagaagta atttctaaaa aaataaatgc agcaatggcc actgatatac ttttctcttt
    32221 tgatacactg aaagctctgt tagccgtcag ataaataata aaaataaaaa ttatattcag
    32281 aaataaagat atactacggc taaccatata gatacttaaa atatcatcgc ttgtcaatcc
    32341 aataagttta ctagcacctg ccactaaata ataataaaga ggcgaatatt gggcaattgc
    32401 atatggtgct ctttcaggat tagtatagat aggcaggccg tcaagcaatc gttgaataaa
    32461 ataaataata tttccttcga ctccaccggc atcaggatgc tgataaaata tcagtattat
    32521 cctgtaaata aagtaaacca agaataagaa aaatgaaacc acggttatgg aacggtcgga
    32581 aaatatacaa gtattcatat taatacagtg aaagataaat aattaactta ggatacctag
    32641 catgatcctc attgttcttt tgcagcacat ctgcagaatc ttactaaacc ctctggttca
    32701 ggctaaaaaa gctcatgttc aatatgtcct gacttatcgt agtggagttg gtatacttta
    32761 ttctttggtt attttaaaag gaatagcctt tttagaagaa tacttgatta gcctttgttc
    32821 ttagaaaaag tcctttccct ttttacaaaa atcatttctg ttactgatta ctatcattgt
    32881 agaaaatcca tacttagaat tatctttaat tgcaattttt agttatgaaa acaatcatca
    32941 ttcccaccga tttttctcct actgccacca atgccatgca ttatggcatc gatatggcaa
    33001 aagctatcca tgcaagtgtt attttattgc atgtttacca ggtgccggtc agctatactg
    33061 acacaccgat tgtacttgta tcggtagaag aattgagaaa ggaagctgag caaaaattgg
    33121 aaaaattaca caaagaagta gagcacctta cttcaggaag catcaaaatt tatacagaag
    33181 ccaggctggg taatattgca gatgagatcg aaaatttgtg cgaaaaagta aaaccctttg
    33241 ctgttgtgat gggcagcaag ggaagcacag gaattgaaaa aatattattt ggtagtacaa
    33301 cacttacagc tatcaggcat cttacctggc ccgttatctg tgtgccgcct ggaaaagttt
    33361 acggaaacgg tattaaaaaa ataggtttcg cctgcgattt cagaaatatt gtggaaacaa
    33421 ccccagcaaa cgcaatcaaa acatttgtaa aggaattcgg tgccgaatta catatattga
    33481 atgtagatta tcacaacaaa caattcaagc ccgaaacacc tgaacagtct gcacttcttc
    33541 acaccatgct ggaagaagta aaccctcttt atgactttat tgagcacagg gatattgaag
    33601 atggtttaaa tgaatttgca gaaaaaaaca acctcgattt actgataacg atacctaaga
    33661 aacataagtt gttagacggg ttattcaagc caagttctac aaagcaactt gttgttcagt
    33721 cgcacatccc cgtaatgtgc atccatgaat agagaaattt ggtagggcag aaataatttg
    33781 taccttttct gttcaccaaa accaaaccac catgaaatgt aaacttaatg cgggggtgtt
    33841 attcctgtct gctatgttat ctctttatgc ctgtggcgga aaaaacaaaa aagctgatga
    33901 caagaaagaa acaactacag aaactactgt aaaagacagc ggtggagatg atggtattgt
    33961 tccaaatatt gatactgctt cattaaagga tgaagcatct atcctggatg ctatgcaaaa
    34021 agtggcagat gcacggctgg cagatgagaa aaaaaagcag gaagacccta attattccgg
    34081 tcattatctt gaactaacca aaatctatac cgctgttttg aaagcttcta cagcatattc
    34141 aaagaccctt accgacccca ataaagcaat agaattcaat aacaaggtta gcgagattca
    34201 gaagaaaatg tatgcaaaat aattctgtag aaaagagctg agcagtcagc tcttttttat
    34261 tgttgcccat atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttataa gaagtcccag
    34381 gaccaggttt aattaaaatc attaattcat ctcttccatt tattaaattc tcgtttttgt
    34441 caaggcttat ttgcatccta ccaataacag ctctaagtga gttttttcca gaaagctcaa
    34501 caggttcaac ctttaggttt aaaacagctt cttcccattc tccctggtaa taataaattg
    34561 aattgccttt tgtcaaaact gtcaatgccg ctgactttgc aaggtccgtt ggcggccctt
    34621 ctgccgtcat agccaaatcc ataacggtgg gtttgcttaa ttct
//
LOCUS       DAORLS010000006        29060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_892_length_29060_cov_2.516187, whole genome shotgun sequence.
ACCESSION   DAORLS010000006 DAORLS010000000
VERSION     DAORLS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 29060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29060
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_29060_cov_2.516187"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PK977_01085"
     CDS             <1..575
                     /locus_tag="PK977_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16721.1"
                     /translation="TAFFYLSSKPAKLLSDVYEKTNCDSTLKYAKLIKTANDSLTSNK
                     TNQQILLMTFEEDLRQQEVAAEKIKEDQQRKQNIQYALLALGIITFVISFLLLSRKHI
                     TNTKLIQFLGVVALLLVFEFLNLLLHPFLERITHHSPVLMLLALVCIAALLVPLHHKL
                     EKWATHKLVEKNKQIRLASAKKTIEELEKK"
     gene            720..1265
                     /locus_tag="PK977_01090"
     CDS             720..1265
                     /locus_tag="PK977_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16722.1"
                     /translation="MDQTTLGIGTRLQHTQHGPGVIVGVKYATYLISFIHHGIREIDK
                     TDTNLEEVIPENVSTEVETQSETEKSLLKILKMWGGITETVPLGDKWIKGMMLLQPFD
                     KTLKPKEIPIEDFFHKIVMLRDRLRVLEQNINSSKNLSDEEKVNIQQYITRCYGSLTT
                     FNVLFKNKEQWFVGDKGNKEE"
     gene            complement(1337..1546)
                     /locus_tag="PK977_01095"
     CDS             complement(1337..1546)
                     /locus_tag="PK977_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRF16723.1"
                     /translation="MKPNMGTVDRIIRVLLAAVFAYLYFSGTVTGTLGLVLVILGGVF
                     VLTSLISFCPLYTLVGLNTCPRKQP"
     gene            complement(1711..>2171)
                     /locus_tag="PK977_01100"
     CDS             complement(1711..>2171)
                     /locus_tag="PK977_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF16724.1"
                     /translation="AEEKELLLYYIGPAQSCIMSFSAGLTNAPSRIFALTEDPSLLLL
                     LPSEKLAKWVKQYPQINELFFQQYNLRYTEMLDTINSLLFDKLDQRLYNYLLEKSKLK
                     GEKILPLRHKHIAGELGTAREVITRVIKRLEQEGKVRQLPEGIEIIQAGD"
     assembly_gap    2172..2187
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2188..2372)
                     /locus_tag="PK977_01105"
     CDS             complement(<2188..2372)
                     /locus_tag="PK977_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16725.1"
                     /translation="MPLAITEIKKFLPGFENELLEEIASHGQIKEVPEGVEILKEGQY
                     VKVIPIVLEGLVKTFTRA"
     gene            complement(2594..>4860)
                     /locus_tag="PK977_01110"
     CDS             complement(2594..>4860)
                     /locus_tag="PK977_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF16726.1"
                     /translation="TPLFDANKQVTGALLVFSDNTEKKRIEKKLQQREKYLRDIIQNL
                     PEPVYTCDADGYIQLYNKAAAELWGQEPKPGRDQWTGAWKVLRPEDKTEIPRDLIPMA
                     IVVRNKIPVYGEQIIIQRPDGSLRHVITYPTPLFDEDGKLTGALNMQIDITDRKEREI
                     MVKKTEAKYQNLVEQASDAIVIYSLDGTIYEFNKAASQLTGYSKEEFRKLGLEDLLAD
                     GPIIKNMEVVNKLLGGEPVIFYRQIRKKDGSAAAVEINASLQADKKLLAFIRDITDRK
                     NAERALKESELFSRSILTAISSHIGVMDVNGTILAVNKAWSDFADHDCMSGLKRPATG
                     TNYLEILKKAAEADDLYAAKALTGIQQVFKGETGSFEMEYPCHTFTQKQWFIMSVNPF
                     AEDSSRVVLRHNDISQRKNLEIKMQAALERYNLLAKATSDTVWDWDIKSDRIIYNEGI
                     TRMFGYDMRKIENTTGWWKQNVHPDDQLTVYEQIDDAIAGKKETVQMQYRYKCADGSY
                     KYIFDRAILLYDKNFRPDRMIGAMQDVTYQKEEDMRIDRAIIRAQEEERKQIGMELHD
                     NVNQLLSASLLYLSMADGATESNISFYDIVKNSRHNISEAIADIRRLSHQLAPATYEH
                     IAVNEIFESLINSVKAARGFDITLEVKGFENKKIQTDIQLNLYRVLQEQLNNIVKYSK
                     AKKVDVQLICVDNKARLLIADDGVGFDPAAIKNGIGLENIRRRTKLFNGHVEINAAPG
                     KGCKVLVELPFQKN"
     assembly_gap    4861..4881
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4882..5856)
                     /locus_tag="PK977_01115"
     CDS             complement(<4882..5856)
                     /locus_tag="PK977_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRF16727.1"
                     /translation="MRKEQKQKWIVMNRKRVLLFVFFIAIALLGAATFFVTQKNRWLG
                     KEYDRREEFIGKTDLLAGINLKIREAESDARGFVLTGNKIFIAGIDKTIDSINKATRQ
                     ILLLHSSPAEHKHTQLFSRLDSLVKLRVALLQEAVLLAETDKKKATEHIATARGKQLT
                     DQVAAVSNEIVRDYEKEIRLSEKGFWNEVSNSNAFVYLSMAVALLLILVSFYFLLKEI
                     DTGKKLRNELRQQKEYLSTTLTSMEEGLITTDEAGRIVYMNPAAEILTGWKTTEAKNQ
                     PLHKVYDVFSESSGQPVDNIVSKILRYGKPVEFENNTILKTRYNSEIII"
     gene            complement(5911..6279)
                     /locus_tag="PK977_01120"
     CDS             complement(5911..6279)
                     /locus_tag="PK977_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16728.1"
                     /translation="MTVAIFETSPYISERLVDLIAEKNEGVVFKKYSNRAEAVEYLGN
                     PLPGAILMDVRFVGSYFDQVFKNYKSNTGTRLIIMHQVIDEPTREKYLAIGTDHMVDL
                     YHDFEKIPVLIREISLARNL"
     gene            complement(6428..7063)
                     /locus_tag="PK977_01125"
     CDS             complement(6428..7063)
                     /locus_tag="PK977_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF16729.1"
                     /translation="MKRIIVADDHSFVRVGLIQILKDEYPQAEIKEVGDGESLVKEVM
                     KQDWDLVISDLDMPGRSGLEALEQIKLIRPALPVIILSIYSEDLYAVRALKAGASGYL
                     NKNAAPYELIKAVERVSMGRKYITPELAEKLLEFGDTGKPHEKLTNREFEIFKMLAAG
                     KTISQIAETLSLALTTVSTHRSHILEKLGLGTNAELTRYAIAHHIISDLKD"
     gene            complement(7076..10666)
                     /locus_tag="PK977_01130"
     CDS             complement(7076..10666)
                     /locus_tag="PK977_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF16730.1"
                     /translation="MKIQATIFRRPLLVGLVVFIILLSLTQYLTYQRYLLRRNAEQKE
                     VTSVVNAAKERMQATLNYSFAASKILAFVVENFGEPRNFDSLAYSLLRSNKFIDAVQL
                     TRKGTIIQIYPLKGNESVIGYDILADSNRNREAIKAIKKRELFFSGPFELKQGGLGIV
                     GRQPIFINDEFWGFAAVVIRLPTLLKEAGISNSESSDFAFELSKINPNTQQEEFFLNY
                     PAKFNESSYLVVDVPDGEWKLYVQRKQTTSFEDILPFGLLGLFLSVTGAIFAWHRTRQ
                     PLKLRQLVNEQTEKLSASEKNYRTLLESITDAFVALDKNWRYTYMNKKAGQVFGRDPA
                     KMIGRHIWTEFPEGLGQPFHKAYELAMQKGQYIYLEEHYEPYDLWFENHIYPTDNGIA
                     IFFQDITERKKAEAKIIKMSRLYDFSRQVNQVIIDVTNEPALFKSICDVAVSVGRFKM
                     AWIGMVNEELHTVDPVSFSGEERGYLVQIKKITTENIREGKGPTGTALREGKAIVCND
                     IETDPRMEPWKVEALSRGYLSSISLPVKKLGRVIGSLSLYAAEKNFFDQEEMEMLERL
                     AANISFSLDNYEKEKLRRQAEVSLKTSEAFTQSIINASPDIIYIYDIEERRNVYINEG
                     MQKMLGYSDSEIKNLGDNVIAHLMHPDDFSIYLAHTYPQYNDLPDDQLLVHEYRMKDK
                     KGEWHWLHTKESIYQRNNDNTPKQIFGVAADISERKRAEQEIIHSNEMLELAEEHAGL
                     GSWEFDVVKQQGRWSKQMFRLFGLPIREFPPDFDEYLDLIHPDDRWLIKTALDDMAAG
                     RDPVSHLFRTNPELSALRYLMPSWFAEKDNNGQTIRYSGTVYDVTKQHLAETQIISEK
                     ELSDSIINSLPGVFYLYNRQGKFYRWNKNFKNVTGYTDAEIENMHPLQFFDTPDKDLL
                     AQKINSVFLNGEDAVEADFLLKSGDKIPHYFTGRSIDYHGELCLMGVGIDISERVRAQ
                     EEIRQASEQLRQLTAHLQRIREEERKRIGREIHDELGQQLTAIKMDVAWIDKKTPAEA
                     GQVKLKLKNIITLLDSSNLSIRKILNELRMGILEHQTIEEALRWQSQQFTDNTGIPIE
                     FNCPRPISKTDEMTATCLFRVFQESLTNITRYAKAKKVITWLTQDEQYVSLSIEDDGK
                     GFDASQLSTTKSFGILGMKERVASLNGKFELITAPGNGTRIEIKIPVNLT"
     gene            10857..>12775
                     /locus_tag="PK977_01135"
     CDS             10857..>12775
                     /locus_tag="PK977_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF16731.1"
                     /translation="MSPTRQLGAIMFTDIMGFTAMMEANEQTANEWRTRLRQSLKTHV
                     SFFNGRIIELNGDGALTFFQSAYEAVKAAIAIQLDMKMNSPVPLRAGIHAGEVVMEES
                     SVYGSVVNVASRVESFAVPGSIFISEKVFDEVKNKPDIQTLSLGKFRFKNVEDPVEIF
                     AIRNEGIAVPDKQKLTGKGVVVEKNKFLLPKAALAGIGATLLITVAVYFLFIKPGDKK
                     LTAVESIAVLPFDNQDKDVQSDFFADGITEDINTNLSRISSLTVISHTTMQAFKKTAK
                     GIKEIGKELNVNSVLKGSLRRMGDKIRIYAELVDVATQKSLWSETIEKDYAKIFDIQS
                     EIAQQIANKLAVKVSEAEKKRIEQKPTDNLQAYEYYLQGRDYYYHYKNEDNEKAIEYF
                     KKAIALDNNYSLAWAGLGDAYSQKHSRYGAEAAWLDSSKIAGQRAIQLDSNSSEAYKA
                     LSNAYYYAKDYDKGFELLEKSVALNPNNAQAVGNLGTAYFLKAQLDEALRWEKKAAAI
                     NPKNGIPYMVIGWTYRILGDYTQAEQWLRKSLELRKFIDTYRELAYTFLLQQKVDSAR
                     NIVPLIIAIDSSNNRNFEVAGMITHMIGDTITAKRYFEKSVSLNKSISTDIEGYEAVS
                     LGQLLLNEGNTIVAD"
     assembly_gap    12776..12890
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13280..13299
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13300..14703
                     /locus_tag="PK977_01140"
     CDS             <13300..14703
                     /locus_tag="PK977_01140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRF16732.1"
                     /translation="YFNAARFIDARGMIVAPGFIDVHTHLEGDEAKDPNATNFILDGV
                     TTCVTGNCGSSNTDIGKYLRWVDSLKLSINVASLIGHNDIRKAVMGRANRDATADEMK
                     RMEDITEKAMKDGAVGLSTGLIYIPGTYTKTPEIVALAKIASKYNGVYATHMRDEGDS
                     VTYAIEEALTIGREAYIPVEISHFKLSGQHNWGRSKVTVPMIEKARKEGIEVTIDQYP
                     YTASSTSISTLIPDEILADGQDSIKARLQRPEVKKYVVKSILERLKKRKLKHLSYAVI
                     AYFAPDTTYNGKSIEQINLMKGRKHKAKYEAETVIDIMMAGGASAVFHGMSEDDIKRI
                     MQYPFNMFASDATIRVLNAGMPHPRGYGTNARVLSKYVREEKVISLEEAIRRMTSLPA
                     QKFQLKDRGLLKEGMAADIVIFDENEVKDVSTFEKPHAYSKGFHFVIVNGVLTVNYEK
                     HLGVRAGKALYGPGTVK"
     gene            complement(14706..16052)
                     /locus_tag="PK977_01145"
     CDS             complement(14706..16052)
                     /locus_tag="PK977_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HRF16733.1"
                     /translation="MSNQPAATLAAPVSQGERIVLLDSLRGLALLGILLMNIPYMGLP
                     EPSFDNPTLNYEMGTINEKVWWYVNWIPEGTQRAIFSMLFGAGIILFVARLEKRTEGI
                     WPAEYFIRRQLWLLVFGLVNAFILLWPGDILFQYSIIGVVAFVFRRLAVKGLLIAAAV
                     CLLLMTTRENIDLHRQKDKIYKGEMAAKIDTTKVKLTDLQQEQLEAMTGMKEKSDTSG
                     LRNEMRKNLQKATGSYSTLYEYMSNISVKLEFYYTYYGIWDILLFMLLGMAFFKSGIL
                     TGQAPAKLYWVLFIVGMGSGLLLTWYRLQFMVDHQFNQFNIAKDKQFEFYEISRTLRS
                     LGLFGLIMLLYKSGWFKWLFALMRPVGQMAFTNYLMQSLMCGLFFFGVGLGMMGKLQR
                     YEIYYVVAAVWVIEIIWSHLWLRFFRFGPLEWCWRSLTYWKKQPMRKANTKEVLSV"
     gene            complement(16082..16378)
                     /locus_tag="PK977_01150"
     CDS             complement(16082..16378)
                     /locus_tag="PK977_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16734.1"
                     /translation="MYIVRDIFYLQFGHYRPAKQLLDEALKGGLLPDNKNARVLSDFT
                     GDAYRLIFEEGFETLTEYEQSLTGSMRKDEWQQWYEKFKPHVVRSHRELLKQVV"
     gene            complement(16488..>16686)
                     /locus_tag="PK977_01155"
     CDS             complement(16488..>16686)
                     /locus_tag="PK977_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF16735.1"
                     /translation="HEDTLIGGIGGEIAAWIAENCFELLDAPVMRCASLDTPVPFSIE
                     LEQNFLAKSRLNETIQKLMSY"
     assembly_gap    16687..16764
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16765..18493)
                     /locus_tag="PK977_01160"
     CDS             complement(<16765..18493)
                     /locus_tag="PK977_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF16736.1"
                     /translation="MYFERKGLTDEALTHFYRSILWPRMIEEKMLVLLRQGKISKWFS
                     GIGQEAIAVGATLALQEDEWIMPMHRNLGVFTTRNMPLSRLFMQWQGAKEGYSKARER
                     SFHFGSREHHICGMISHLGPQMAIADGVALAYKLKKDDKVTLAFTGDGATSEGDFHEA
                     LNVAAVWDLPVIFLIENNGYGLSTPVNEQYRCISLVDKAKGYGMEGVQIDGNNLLAVY
                     DTIKGVREFCIKHQKPYLIECMTFRMRGHEEASGTKYVPQDLFRLWEQKDPIKNYEHY
                     LFNTGVLSEMKVAEIRNEFKEKIESELAIGFNTKPVVADTEEELEDVYAKVQGSISKA
                     HVVDNSRPGFDSRLPTKDSRLIDAIKEGLHQSMQQHPNLILMGQDIAEYGGAFKITEG
                     FVQEFGKARVRNTPLCESAIVGTALGLSLEGYKSMMEMQFADFVTVGFNQIVNNLAKI
                     HYRWGQNADVVIRMPAGGGVGAGPFHSQSNEAWFTKVPGLKVVYPSTPMDAKGLLIAA
                     INDPNPVMYFEHKALYRSVSGPVPEEYYAIEIGKARHVREGDEVSIITYGSGVHWAED
                     YAAEHPEISI"
     gene            18688..19851
                     /locus_tag="PK977_01165"
     CDS             18688..19851
                     /locus_tag="PK977_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF16737.1"
                     /translation="MRKVFTLVQLLCFFFASAQDLNWNTVSYTTGSLSANFGLIGSPA
                     STVTFTVTGNTNRIDANYPVKYIANPSGSANDCAVNCAVRSSVTFTTLSESVVYEFNF
                     SPAVTGLSFRIYDIDGTNASSGDRAIVTAVNGTTAQNITMTTSSGPTITGSGTTTATA
                     TGTQGNTTDDFINVSIISGVTRLSVAYSNNPNNGSPGNRSFSIGNMSWSGVLPVKWVS
                     FTGRKQINGSVLLNWVTASETNADKYIIEKSMDGQRFNPVSELPATGRNNINYYSFND
                     AGPIAGTVLYRIKQTDFDGKYDYSGIVMIKQQAGLQKSLVFPNPATDMLTITLPGNVQ
                     LKNVKVFDVNGRLVMDAVNTSNLLNIKRLSKGLYSLQAENTSGEIFSSYFIKQ"
     gene            complement(19855..21129)
                     /locus_tag="PK977_01170"
     CDS             complement(19855..21129)
                     /locus_tag="PK977_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF16738.1"
                     /translation="MPDRKHAPEIVDAVNFKLQLKQAEKFVLKNGVEVYAVDAGAEEV
                     LAVEWVFYAGNWFEEQNLVAATTNFLLRNGTSKKTAFQINEYFEYYGSYLNRACYNET
                     ATITLHCLSKHIKELLPSVRELIVDSVMPQEELDIYKQNMKQRLKVNLKKSDFVASRL
                     IDVYLYGENHPYGKYSKLEDFDTLNREQLLDFYKKYYQQGKLVLFVAGKLPADIASLL
                     DQYFGDLPNQPVALQEIMAQPAADRKYRITNDEAGVQGSVRMARSFPNRHHPDFMKVM
                     VLNTLFGGFFGSRLMSNIREEKGYTYGIHSFIQNHIHDTAWMISTEAGKDVCEATIEE
                     VYKEMKLLREQIVDEEELLLVRNYMMGGILGDLDGPFQIMGRWKNIVLNNLTEQYFYD
                     SIHTIKTISAEELQFLAQKYLKPEEFYELVVI"
     gene            complement(21159..22397)
                     /locus_tag="PK977_01175"
     CDS             complement(21159..22397)
                     /locus_tag="PK977_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF16739.1"
                     /translation="MIQFEKFTLANGLRVIVHQDLSTPMAVMNIMYDVGARDEDPERT
                     GFAHLFEHLMFGGSENIPSYDEPLQMAGGENNAYTTNDLTNYYIQLPAENLETAFWLE
                     SDRMLSLAFSEKSLDVQRKVVCEEFKEHYLTKPYGDVWHKMRELAYKKHPYRWMTIGK
                     ELSHIENAKLDDVKNFFFKHYRPVNAVLVVAGNVTVERVKELAQKWFGDIPSGEKYNR
                     SLPVEPEQTEERFLEIKANVPLDALYKCWHMGSRMDSSYYAVDLMTDILSGGGSSRLY
                     QSLVKEQKLFSNIECHHFGSTDAGLVVVEGKLVKGVKMEDAEKAVEKELEKMKNEKVA
                     DAELQKVKNKTESMIAFEDMSVMSRANSLAYYETLGDAAWMNFELQKYAKVTTEDILE
                     QSRQIFRRENCSTIHYLSQN"
     gene            complement(22469..23668)
                     /locus_tag="PK977_01180"
     CDS             complement(22469..23668)
                     /locus_tag="PK977_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HRF16740.1"
                     /translation="MRKKAIYLFFTGIMLLAGNSLKAQEEFIEPPSREITKVPFTQLT
                     GGIVIMQAKLESFPDTLNFILDTGSSGISLDSTTVDYLKMKPTPSDRTIRGIAGIRKV
                     SFLYNQKLHFPGLTVDSLNFHINDYTILTAVYGERIDGIIGYSVLSRYILKVDYDSMR
                     ISFCTKGTIRYPRGGYLLKPTINQLVSQPLRVRDEKTIYSRFLYDMGAGLCMLLSKEF
                     AEDSNFLSKKKKRFIKEGEGLGGKIDMELMLMREVKIGPYKFRNVPTFIFDDENNVTS
                     YPYMGGLIGNDILRRFNIILNYAKGDIFIIPNRHFNETFDYSYSGIELYFINGLIIIG
                     DVAKGSPGEAAGLKEGDEVIAINKNFSQNLNQYKIALQVPGEKVKIVFRRDSRLMEVE
                     FKVKSIL"
     gene            23806..24930
                     /gene="mqnC"
                     /locus_tag="PK977_01185"
     CDS             23806..24930
                     /gene="mqnC"
                     /locus_tag="PK977_01185"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HRF16741.1"
                     /translation="MQLNELYDKAAAFGFLSVEEGVHLYHHAPLADLMFIADELRKKQ
                     VPHGKVTWQIDRNVNTTNVCIANCKFCNFYRIPGHAEAYITDMPTYRQKITETLKYGG
                     DQLLLQGGHHPELGLQFYVDTFRQIKAEFPNIKLHALGPPEVAHITKLEKSTHHEVLK
                     ALKEAGMDSLPGAGAEILIDRVRRLISKGKCGAQEWLDIMHEAHKLNITTSATMMFGH
                     VETIEERFEHLVKIREVQSRKPEHANGFLAFIPWTFQDVDTLLAKIRGVQNLTTAEEY
                     IRMIALSRIMLPNVKNIQASWLTVGKQTAQICLHAGANDFGSIMIEENVVSAAGAPHR
                     FTYRSMQEAIREAGFEPQLRNQQYEWREIPETIEEQMIDY"
     gene            25040..25345
                     /locus_tag="PK977_01190"
     CDS             25040..25345
                     /locus_tag="PK977_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRF16742.1"
                     /translation="MKRTFTWFRKTAFAEGISFIVLLFVAMPLKYFAGMPMAVTIIGS
                     AHGALFVAFMALAYMVKEQYNKSLGWGVKAFLASIIPFGTFYMEKQWKKEEAEGAAG"
     gene            25470..26612
                     /locus_tag="PK977_01195"
     CDS             25470..26612
                     /locus_tag="PK977_01195"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF16743.1"
                     /translation="MTKYILIILLLISPFIGQAQAENQNLPNDNLMKSFTDSAVQKSV
                     LPFMSDSLRVGLSIGIYQNNKVFTYNYGSTQKGKQILPTNNTIYEIGSISKTFTGTLL
                     AQAVQDKKVKIDDDIRLYLDNSYPNLEYRGNPIRLSHLVSHISGLPLFLPDIPTLFQN
                     ANFDTLPFTISKIQENYSKEKFLDDLHNVKLDTIPGFKFRYSNAGAQLLKYILEKVYK
                     MPYDKLLKKYISNPLNMINTNSLFSNNNLNLLVKGYNDKGNIMPYNPQILDAAGGIFS
                     TVSDMLNYIRFHLDETNPVVALSHKAVKGNINDYAVGLNWQEMTTPKKHKKIWQSGGT
                     FGFSSYCVVYPELNFGIILLTNESDRMTQGRLEEVADKIFEKINSQ"
     assembly_gap    26970..27104
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27105..27588
                     /locus_tag="PK977_01200"
     CDS             <27105..27588
                     /locus_tag="PK977_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16744.1"
                     /translation="KGKGYLPWGNYSDIVFYNSETNQSKKLFGDKLALINTFSQRRNY
                     YYYDESREKEIPANILRHHIIYLVRTENFNNDGGLDTDDPVYLYISTKTGDNLRQITP
                     GGFHVLSWTVSKDQKMILVKGQNDKNGNKKFGQGDDQLYYRIDLEDDVSKIRCYPLNF
                     "
     gene            complement(27597..>28295)
                     /locus_tag="PK977_01205"
     CDS             complement(27597..>28295)
                     /locus_tag="PK977_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF16745.1"
                     /translation="IPWIIHQYNKGAEVASLCIGAFLFAATGLLDGKRCSTHWSRSNE
                     FRKMFPRTELVDSCIITEEQGLYTSGGANSYWNLLVYLVEKYTDRATAILAARYFAVE
                     IDRDSQAAFMLFNGQRTHEDEEIKKAQDFIEEHYNDKLSVDQLAGHFAIGRRSFERRF
                     KKATSNTVVEYIQRVKMEAAKRSFETSRKNISEVMLDVGYTDTKAFRTIFRKITGLTP
                     VEYRNKYNKQMLVN"
     assembly_gap    28296..28314
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28315..28583)
                     /locus_tag="PK977_01210"
     CDS             complement(<28315..28583)
                     /locus_tag="PK977_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF16746.1"
                     /translation="MKHVSILVPETAVIEAVADPRYMFSAVNMFLQEAGKEALFNVQL
                     VGLRKEVKLEGSIFSVHTDMLPEEVSQTDLIVIPALSGDMTAAIE"
     gene            28740..>29060
                     /locus_tag="PK977_01215"
     CDS             28740..>29060
                     /locus_tag="PK977_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16747.1"
                     /translation="MASVSTYLNFPQHTEEAFHFYKSVFGTEFTPPGIRRFGDMPPMK
                     GVPPTPDALKNLVMHVELPITGGHILMGTDAPEQMGFTVSYGNNIHISLHPDTRTENK
                     RLFDA"
BASE COUNT         8077 a         6520 c         5660 g         8399 t
ORIGIN      
        1 atacggcctt cttttacctg agcagtaaac cggcaaaact gctttctgat gtgtatgaaa
       61 aaacaaattg cgacagcaca ttaaaatatg caaagcttat aaaaactgcc aacgattcgc
      121 tgaccagcaa caaaaccaac cagcaaatat tgttaatgac ctttgaagaa gatttacggc
      181 agcaggaagt agcagctgaa aaaataaaag aagaccagca acgaaaacaa aacatccaat
      241 acgccttatt agccctaggc attatcactt tcgtcatttc ttttttactg ctcagcagaa
      301 aacatatcac caacacaaaa ctgattcagt ttctcggcgt agtggctttg ctattagtct
      361 ttgagttttt aaacttatta ctccatcctt ttttagaacg catcacccat cattcgccag
      421 tgcttatgtt gctggcattg gtttgtattg ctgctttatt agttccgctt catcataaat
      481 tagaaaaatg ggctacgcat aagctggtgg agaagaataa gcaaatacgg ctggccagtg
      541 cgaagaaaac gattgaggag ttggaaaaga aataatatgt tgctgtaaat tgtcttttta
      601 tttaatacta tatgtttgga gataatacca actggaaagg ccgcctgttg cggtttgtaa
      661 tttatgccgc cgtgggtttt ctttgtgcct ttttgtacaa atacttaaaa aaataattta
      721 tggaccaaac aactctcggc atcggcaccc gcctgcaaca cacccagcat ggccccggtg
      781 taatcgttgg cgttaaatac gccacctatc ttatctcatt catccatcac ggtataaggg
      841 aaattgataa aacagatacc aatctggaag aagttattcc ggagaatgta tccacagaag
      901 tggaaacaca aagcgaaaca gaaaagtcgt tgttgaaaat tttaaaaatg tggggtggca
      961 ttaccgaaac agtaccgctc ggtgacaaat ggataaaagg catgatgctc ttacagcctt
     1021 ttgacaaaac actaaaacca aaagaaatac ctatcgagga tttctttcac aagattgtga
     1081 tgctgcggga caggctgcgg gtgctggaac aaaacatcaa cagcagtaaa aatttatcgg
     1141 atgaagaaaa ggtaaacata caacagtaca ttacccgctg ttatggctca ctcactactt
     1201 tcaatgtatt attcaaaaac aaagagcaat ggtttgtggg agataaagga aataaagaag
     1261 aatgattaaa aagggtgaaa ccggccgatt tcacccttac ctttttaatc acccgctgct
     1321 ataacgcagc agttttttag ggttgtttac ggggacaggt gttcagccct accaatgtgt
     1381 acagcgggca aaaactgata aggctggtca gcacaaatac gccacccagt attaccagta
     1441 ccaatcccaa agtgcctgtc actgtgccgc tgaaatacaa ataagcaaat acagcagcca
     1501 ataatacccg gatgatgcgg tcgacggttc ccatgtttgg tttcatagcc aataattttg
     1561 ttgtgattaa aaaagtttta tcaaaaaaac aattccggca acagctgcca ggcagaggat
     1621 acaaacaata accagttttg cccattgcgt tttcattgcc tgaattgatg ctgtaaaact
     1681 aaaaaccgtg aacaaggtgg tacggtgaca tcagtcacca gcctgaatga tttctatgcc
     1741 ctccggcagc tggcgtactt ttccttcctg ctccagtcgt tttattaccc gggtgatgac
     1801 ctctcttgcg gttcccagtt caccggcaat gtgcttgtga cggagtggta agatcttttc
     1861 acccttcagt ttgcttttct ccagcaaata attatacaaa cgctgatcca gcttatcgaa
     1921 caataaggag ttaatggtat ccagcatttc tgtatagcgt aggttgtatt gctggaaaaa
     1981 aagttcgttg atctgcgggt attgtttaac ccatttcgcc agtttttctg aaggcaataa
     2041 taaaagcagc gaggggtctt ctgtaagggc gaatatgcga ctgggcgcat tggtcagccc
     2101 ggcagaaaac gacatgatac agctttgggc cggcccgata taatataaaa gcagttcttt
     2161 ttcttctgcc cnnnnnnnnn nnnnnnngcc cgggtgaatg tttttaccaa cccttccagc
     2221 acaatgggaa tcacttttac atattgtccc tcttttagta tttccactcc ttccggcact
     2281 tctttgatct gtccatgact ggcaatctct tccagcaatt cattttcaaa gcccgggagg
     2341 aattttttaa tttcagtaat ggctaacggc atgatcaggt tgattaagga ataaatctac
     2401 aacaagtgct gtgttggcgg caaatcaaag ttttattccc taaaaatata taccctattt
     2461 ggcattcttg tttttatccg gtaagtactt atgagcacaa cctggttaaa gtattttaac
     2521 gacagccctg tggaatgggc atggccaagt tcacctataa ataatagtat cagatgagga
     2581 aagataaacg gggttagttc ttctgaaagg gcaattccac caatacctta caacctttgc
     2641 ccggcgcggc attgatctcc acatggccat taaagagctt ggtccttctg cggatatttt
     2701 ccaggccgat accgttcttt attgctgcag gatcaaaacc aactccatca tcggcgatta
     2761 atagccttgc cttattatca acacagatca gctgaacgtc tactttttta gctttcgagt
     2821 atttgacgat attgttcaat tgctcctgca gaacccggta aaggtttaac tggatatctg
     2881 tctgaatctt tttgttctca aagcccttta cctccagtgt tatgtcgaag ccacgggccg
     2941 ctttgacact gttgatcaat gattcaaata tttcatttac tgcaatgtgc tcatatgtgg
     3001 ccggggccag ctggtgagac agccgtcgga tatcggcaat cgcttcactg atattgtgcc
     3061 ggctgttttt tacaatatca taaaaactga tattgctttc tgttgctccg tcagccatac
     3121 ttaggtacaa cagcgaagcg gataataact ggttcacatt atcatgcagc tccataccta
     3181 tctgctttct ttcctcttcc tgtgccctta tgatagcccg gtcaatacgc atatcctctt
     3241 ctttctggta ggttacgtcc tgcatagcgc caatcattct atccggtctg aagtttttat
     3301 catatagtaa aatggcccgg tcgaaaatat atttgtaaga accatcagca catttatagc
     3361 ggtattgcat ttgtaccgtt tctttcttac ctgcaatagc gtcatcgatc tgttcataaa
     3421 cggtcagctg atcatcaggg tgtacatttt gcttccacca tccggtagtg ttttcgattt
     3481 ttcgcatgtc ataaccaaac atgcgggtta taccttcatt ataaataatc ctgtctgatt
     3541 ttatgtccca gtcccatacc gtgtcgctgg tcgcttttgc cagcaggttg tatcgttcga
     3601 gggcagcctg catttttatc tccaggtttt tacgctgaga tatatcatta tgccgcaata
     3661 ccaccctgct gctgtcttca gcaaaggggt tcacactcat gataaaccac tgtttttgtg
     3721 taaaggtgtg acaggggtat tccatttcaa agcttcctgt ctctcctttg aacacctgct
     3781 gaatgcctgt cagcgcctta gcagcgtaaa gatcatctgc ttcagctgct tttttcagta
     3841 tttcaagata attggttccg gttgcaggcc gttttaaccc ggacatacaa tcatgatctg
     3901 cgaaatcgct ccatgcttta ttgacggcaa gaatagttcc attgacatcc attacaccga
     3961 tatgtgaaga gatcgctgtc aatatactcc ggctgaagag ttcactttcc tttaatgccc
     4021 tttctgcgtt tttgcgatcg gttatatccc tgatgaaggc cagcaatttc ttgtctgcct
     4081 gtaaactggc attgatctca acagccgctg ctgatccgtc ttttttcctt atttgcctgt
     4141 aaaaaatcac cggttcacca ccaagtaatt tgttgaccac ttccatattc ttaatgatcg
     4201 gtccatctgc cagcaaatcc tctaaaccca gttttctgaa ttcttcttta ctatacccgg
     4261 taagctgact ggctgctttg ttaaattcat aaatggttcc atccagggag tagatgacta
     4321 tggcatcaga agcctgctca accaggttct ggtattttgc ctccgttttc ttcaccatta
     4381 tctccctttc ctttctgtca gttatatcaa tttgcatatt cagtgcgcca gtcagttttc
     4441 catcttcatc gaacaggggg gtagggtaag tgatcacatg ccgaagactt ccatccggcc
     4501 gttgaattat tatttgttct ccgtatacag gtatcttatt cctaacaaca atagccattg
     4561 gtataagatc ccgtggaatc tccgttttat cttccggacg cagtactttc caggctccgg
     4621 tccattgatc tctccctggt ttaggttcct ggccccatag ttctgctgct gccttattat
     4681 ataattgtat gtagccgtcc gcatcacaag tgtaaaccgg ctcaggaaga ttctgaataa
     4741 tatcccgcag gtatttctcc cgctgctgca gcttcttctc aatccttttt ttctcggtat
     4801 tatcactaaa aaccagtaag gccccggtta cctgtttgtt tgcatcaaat agcggggtac
     4861 nnnnnnnnnn nnnnnnnnnn naataatgat ctcactattg tatctcgttt tgaggattgt
     4921 attattctca aattcaaccg gctttccgta cctcagtatt ttgctgacga tattatcaac
     4981 gggctgaccg gaggattctg aaaatacatc gtatacttta tgaagcggct gattttttgc
     5041 ctctgtcgtt ttccatcctg taagtatttc cgctgcgggg ttcatataaa cgatcctgcc
     5101 ggcttcatcc gttgtgatca atccttcttc catgctggta agggtagtgc tcaggtattc
     5161 tttttgttgt cttagttcat tacgcagctt tttaccggta tctatttctt tcagtaaaaa
     5221 gtagaaggat accaatatca gcaggagggc aactgccatg ctgagataga caaaggcatt
     5281 gctattagaa acttcgttcc aaaatccttt ctctgataac ctgatttctt tttcataatc
     5341 ccgcacaatt tcattgctta cagctgctac ctgatcagtc agttgtttgc cccgggctgt
     5401 tgctatatgt tctgtagctt ttttcttatc tgtttctgct aacaatacag cctcctgcaa
     5461 taaagctacc ctgagtttta ccaaactgtc cagcctggaa aaaagctgcg tatgcttatg
     5521 ttcagccggt gaactatgca acagtaatat ttggcgggtt gccttgttta ttgagtcaat
     5581 tgttttatca ataccggcaa taaatatttt gtttcctgta agtacgaatc cccgggcatc
     5641 agactcggct tcacgaattt tgagattaat ccctgccagt aaatctgttt ttccgataaa
     5701 ttcttcccgc cggtcatact cctttccgag ccaacggttt ttctgtgtta caaaaaacgt
     5761 agctgcaccc aataaagcaa tggcaataaa aaacacaaag agcaaaactc tcttcctgtt
     5821 catcactatc catttctgtt tctgctcttt gcgcatatag tctgtttcac tccacaggat
     5881 gggaaattaa aaacgggtcc ctttttgttt tcataagttt cttgccagac tgatctccct
     5941 gatcagtact ggtatttttt caaaatcgtg gtaaaggtca accatgtgat cagtacctat
     6001 tgccaggtat ttttcccggg ttggttcatc tatcacctgg tgcattataa tcagccgggt
     6061 gccggtatta cttttataat ttttaaatac ctgatcaaaa tagcttccta caaaccgcac
     6121 atccattaat atagcaccgg gtaagggatt acctaagtat tcaacagctt cagccctgtt
     6181 gctatatttt ttgaaaacaa caccttcatt tttctcagca ataagatcta ccagccgttc
     6241 tgatatatag ggcgatgttt caaatattgc taccgtcata ttgttcttca atggaacatc
     6301 aaagaaccaa caactgtccg atgtaaaaaa tagcagggcg ggccaatagc agttagaata
     6361 atgcacagac aggcatagaa gaactactat gcgggtaggc gaataactac aagacttctt
     6421 ttatgttcta gtctttgagg tctgaaatga tatgatgtgc gattgcgtaa cgggttaact
     6481 ctgcattggt acccaggccc agtttttcaa gaatatggct gcggtgtgta cttaccgttg
     6541 tcagggccaa tgaaagtgtt tcagcgatct gggaaatagt tttaccagcg gccagcattt
     6601 tgaatatttc aaattccctg ttggttaatt tttcatgagg cttgccggtg tcaccaaact
     6661 caagcagctt ttctgccagc tcgggagtaa tgtatttcct gcccatcgaa actctttcca
     6721 ccgccttgat cagttcataa ggcgctgcat ttttattcag gtaaccagaa gccccagcct
     6781 tcagggctct tacagcataa agatcttcgg agtaaatgct aaggattatc accggtaatg
     6841 ccggccttat aagtttgatt tgctctaatg cttccagtcc gcttcgcccg ggcatatcca
     6901 ggtcagaaat caccaggtcc cagtcttgct tcatcacttc tttaaccagt gattcaccat
     6961 cacccacttc ttttatttct gcctgagggt attcgtcttt gagtatttgt atcaggccta
     7021 ccctgacaaa actatggtca tctgcaacta ttattcgttt cataaaaaat atacgttagg
     7081 taaggtttac aggaatcttt atttctatac gggtaccatt tccgggtgcc gttattaatt
     7141 caaattttcc gtttaacgag gctactcttt ctttcattcc caagatacca aatgatttag
     7201 ttgtactcaa ttgtgatgca tcaaaccctt ttccatcatc ttcgatacta aggcttacat
     7261 actgttcgtc ctgtgttaac catgtaatca ccttttttgc ttttgcatac cgggttatgt
     7321 tggtcagaga ttcctggaat accctgaaca aacaggtggc tgtcatttca tccgttttac
     7381 ttatcggcct gggacaatta aactcaatgg gtatgccggt attatcggta aactgctgac
     7441 tctgccatcg cagggcttct tcaatggtct ggtgttccag tattcccatc cgcagttcat
     7501 tcaggatttt gcgtatagac aggttactgc tatccagaag ggtgatgata ttctttaatt
     7561 ttaatttaac ctgccctgct tctgccggag ttttcttatc gatccatgcc acatccatct
     7621 taatggctgt taattgctgg cccagttcat catgtatttc ccggccgatc cgttttcttt
     7681 cctcttcccg gatcctttgc aggtgagcag ttagttgcct gagttgttcc gatgcctgcc
     7741 tgatttcttc ctgtgccctc actctttctg aaatatcaat acccacaccc atcaggcaaa
     7801 gctctccatg atagtcaata gacctgccgg taaaataatg cggaatttta tcgccggatt
     7861 tcaacaggaa atctgcttca actgcgtcct cgccatttaa aaaaacgctg ttgatttttt
     7921 gtgccagtaa atctttgtcg ggagtatcaa aaaattgcag cggatgcatg ttttcaattt
     7981 cagcatctgt ataccctgtt acatttttaa aattcttatt ccaccggtaa aattttccct
     8041 gcctgttata cagataaaat acacctggta aactattaat gatagaatcg gacaattcct
     8101 tttcactaat gatttgtgtt tctgcaagat gctgtttagt tacatcatac accgtacccg
     8161 aataacgtat tgtttgcccg ttattatctt tttctgcaaa ccaggaaggc atcaggtatc
     8221 ttaatgcaga aagctccgga ttggtcctga aaagatgtga aaccgggtct ctccccgcag
     8281 ccatatcatc cagcgcagtt tttatcaacc acctgtcgtc aggatgtatt aaatcaaggt
     8341 attcatcaaa atcaggaggg aattcacgaa tcggcaatcc aaaaagccgg aacatttgtt
     8401 ttgaccagcg gccctgctgc ttcaccacat caaattccca actgccaagc ccggcatgtt
     8461 cctctgccag ctccagcatt tcattggagt gtatgatttc ctgttctgct ctctttcttt
     8521 cgctgatatc agcagcaaca ccaaaaattt gctttggtgt attatcattg ttacgctggt
     8581 atatggattc ctttgtatgc aaccagtgcc attccccttt tttatctttc atccgatact
     8641 catgaaccag caactgatca tccgggagat cattatactg cgggtaagtg tgtgccaggt
     8701 agatactgaa gtcatccggg tgcatcagat gcgcaatgac attatcgccc aggtttttta
     8761 tttcactgtc ggaatatccc agcatttttt gcataccctc attgatatat acatttctcc
     8821 tttcctcaat gtcgtagatg taaataatat ccggggatgc gttaataatg ctctgtgtaa
     8881 atgcctcgct ggttttaaga gatacttcag cctgtcttct taatttctct ttttcgtaat
     8941 tatcaagaga gaatgaaata tttgcagcta atctttccag catttccatc tcctcctggt
     9001 cgaagaaatt tttttcggca gcgtaaagtg acagagagcc tattactctt cccagctttt
     9061 taaccggcaa tgaaatagat gacaggtatc ctctgctaag agcttccact ttccatggct
     9121 ccatccgggg atctgtttct atgtcattgc atacaattgc ttttccttcc cgcagtgccg
     9181 taccggtggg gccttttcct tcccttatat tttccgttgt aatttttttg atctgcacca
     9241 ggtatcctct ttcttcacct gaaaaagata ccggatccac tgtgtgcaac tcttcattga
     9301 ccattcctat ccaggccatt ttgaatcttc ctactgatac agccacatca caaatagatt
     9361 tgaataaagc cggctcattg gttacatcaa tgattacctg gttcacctgc cgggagaagt
     9421 catacagccg gctcatcttt atgatctttg cttctgcttt ttttctttcc gtaatatcct
     9481 gaaaaaagat ggcgatacca ttatcagtcg gatagatgtg gttttcaaac cagagatcat
     9541 agggttcata atgttcttcc aggtaaatgt attgaccttt ctgcattgcc agctcataag
     9601 ccttatgaaa aggctgtcca aggccttcag gaaattctgt ccatatgtgc ctgccaatca
     9661 tttttgcagg atcacggccg aacacctgcc ctgccttttt attcatatag gtgtaccgcc
     9721 agtttttatc caatgctaca aaagcatccg tgatactctc caataatgtt ctgtaatttt
     9781 tttcgctggc acttagtttt tccgtttgct catttaccag ctgtctcagc tttagcggct
     9841 gacgggttcg gtgccatgca aatattgctc ctgttacaga taaaaacagt cccagcaatc
     9901 caaaaggcaa aatatcttca aagctggtag tttgttttct ttgtacatat aatttccatt
     9961 caccatcagg cacatctacc accaggtaac tgctttcatt aaactttgcc ggataattca
    10021 gaaagaactc ctcctgctgt gtatttggat ttatcttgga gagttcaaat gcaaaatcgc
    10081 tgctttcact attactgatt cctgcctctt ttagcaaagt tggcagccgg attaccacag
    10141 ctgcaaatcc ccagaattca tcgtttataa aaataggctg gcggcccacg ataccaagcc
    10201 caccctgctt caactcgaaa gggcctgaaa aaaacaattc cctttttttg attgctttta
    10261 ttgcttccct gttcctgttt gaatcggcca gtatatcata gccaattacc gactcattgc
    10321 ccttcaacgg gtaaatctga ataatagtgc ctttgcgggt cagctgcact gcatcaatga
    10381 atttattaga tcgcagcaat gaataggcca gcgaatcaaa atttcttggc tcaccaaaat
    10441 tctccaccac aaaggcgagt attttggaag cagcaaaact gtagtttaat gtagcctgca
    10501 tacgttcttt ggcagcattg accacacttg ttacttcctt ttgttcggca ttcctgcgaa
    10561 gaagatatcg ctggtaggta agatattggg ttagggaaag aaggataata aaaacaacca
    10621 atcccacaag caatggcctc ctgaatatag tagcttgtat tttcataaat caatgcgcag
    10681 aaaaacaaat atgacacaca aaaagaggca atgataatct tacataaaca gaaaattata
    10741 cagtataaca agttactaaa aattgacaag tgacggagca aatgatgaag caattttgca
    10801 aggcttcctt cagcttagat taagacagta tatttactcc ttaaagaaaa aggcaaatga
    10861 gcccaacccg ccagctggga gcaataatgt ttacagatat catggggttt accgccatga
    10921 tggaagccaa cgaacaaaca gccaatgaat ggcgtacccg tttacgtcag tcattgaaga
    10981 cccatgtatc tttttttaat ggccggatca ttgagttgaa cggtgatggt gcactcactt
    11041 tttttcaaag tgcatatgaa gcagtaaaag cagctatagc tattcagctg gacatgaaaa
    11101 tgaacagccc tgttccttta agggccggaa ttcatgccgg tgaagtggta atggaagaaa
    11161 gcagcgtata tggcagtgtg gtcaatgtgg cttcacgggt agaatcattc gctgttccgg
    11221 gcagtatttt tatttcagaa aaagtgtttg acgaagtaaa aaataaaccg gatatacaaa
    11281 ccctttcact tggcaagttc cggttcaaga atgtagaaga ccctgttgaa atatttgcca
    11341 tccggaatga aggaattgct gttcctgaca aacagaagct gacaggaaaa ggtgtggttg
    11401 tagaaaaaaa taaattcctg ttacccaaag ctgccctggc gggtatcggc gctaccttat
    11461 tgatcaccgt tgcagtttat tttcttttca taaaaccggg agataaaaaa ttaactgccg
    11521 tagaatcaat tgctgttttg cctttcgata accaggacaa ggatgttcaa agcgattttt
    11581 ttgccgatgg aattacagaa gacatcaaca ccaatttatc caggatcagt tcgctgacgg
    11641 tgatctcgca taccactatg caggctttta aaaagacggc gaagggtatt aaagagatcg
    11701 gaaaagagct gaatgtaaac agtgttttaa aaggcagctt gcgaagaatg ggtgacaaaa
    11761 tcaggatata tgctgaactg gtggatgtgg ctacccaaaa aagcctgtgg agcgaaacaa
    11821 tagaaaaaga ctatgcaaag atcttcgaca tacagagtga gatagcccaa cagatcgcta
    11881 ataagctggc cgtaaaagtt tctgaagcag aaaaaaaacg tattgaacaa aaaccaacgg
    11941 acaacctgca ggcttatgaa tattacctgc agggcagaga ttactactac cactataaaa
    12001 atgaagacaa cgaaaaagcc attgaatatt ttaaaaaagc cattgccctt gacaataact
    12061 attcattagc atgggccgga ctgggtgatg cttacagcca gaaacacagc cgctatgggg
    12121 cagaagctgc ctggcttgat tccagcaaga tagccggtca gcgggctata cagctcgaca
    12181 gcaattcatc agaagcatac aaagcattgt ccaacgcata ttattatgcc aaggattatg
    12241 ataaaggatt tgagctgtta gaaaaatctg ttgcacttaa tccgaataat gcgcaggcgg
    12301 tcggtaatct cggaacggca tatttcttaa aagcacaatt agatgaagcg cttcgctggg
    12361 aaaaaaaggc agcagccatc aaccctaaaa atggcatccc ttatatggtc atcggttgga
    12421 cttaccgtat tctcggtgat tatacacaag ctgaacaatg gctgagaaaa tcactggagc
    12481 tcagaaagtt tattgatacc taccgggaac tggcttatac ttttttatta cagcaaaaag
    12541 ttgattctgc cagaaatatc gtaccgctta tcattgcgat tgacagcagc aacaaccgaa
    12601 actttgaagt ggccggcatg atcactcata tgattggtga taccataacg gctaaacggt
    12661 attttgaaaa atcggttagc ctgaacaaat caatttcgac agatattgaa ggctacgaag
    12721 ccgtaagcct tggccagctg ttactgaatg aaggcaatac cattgtagca gatatnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagcaagccc
    12901 tgacctggct gaaaaaagca caggaattca aatggatgga ttatggtttt ggcgaactga
    12961 ccccctggta taaaaacatc cgcaacgaac ccgccttccg gcaactgatg agcaacctta
    13021 agcaaaatgc tgccgacatg agaaggaaag ctgcatcact ttagcccttt ttaccaaagc
    13081 actattactg cttcctcctt tttaagtaat tttccggaat gaaggccctg attatatccc
    13141 tggcattttt gccgcttaca ttgtttgcac aaccaccctg tgatattttg atcgttaacg
    13201 gcaaaatcat tgacggtacc ggcaacaact ggtactatgg caatattgct ataaaagacg
    13261 gtaaaatcat tgccatcggn nnnnnnnnnn nnnnnnnnnt attttaatgc cgccagattc
    13321 attgatgcaa gagggatgat cgtggcacct ggttttattg atgtgcatac ccacctggaa
    13381 ggcgatgaag caaaagatcc caatgccacc aattttatac tggatggagt gactacctgt
    13441 gttaccggta attgcggatc atccaataca gatattggaa aatacctgcg atgggtagat
    13501 tcattaaagc tatccattaa tgtagcatcc ctgatcgggc acaacgacat acgcaaagct
    13561 gtaatgggcc gtgccaaccg tgatgcgacg gcggatgaaa tgaaacggat ggaagacatt
    13621 accgaaaaag caatgaaaga cggagcggtg ggtttatcca ccggccttat ttatattccg
    13681 ggcacctaca ccaaaactcc tgagatcgtt gcgctggcaa agattgcttc aaagtataac
    13741 ggtgtttatg ccacgcatat gcgggatgag ggcgacagtg ttacctatgc tattgaagaa
    13801 gcgctgacca ttggaagaga ggcctatatt ccggtagaga tatctcactt caaactcagc
    13861 ggacagcata actggggaag gagcaaggtc actgttccta tgattgaaaa agccagaaaa
    13921 gaaggaatag aagttacgat tgaccagtat ccttacactg ccagcagcac ttccatcagt
    13981 acattgatcc cggatgaaat attagctgac ggacaagatt cgattaaagc aagattgcaa
    14041 agaccggaag tgaaaaagta tgtggtcaaa tccatactgg aacggttgaa gaaaagaaaa
    14101 ctgaaacacc tcagctatgc cgtcattgcc tacttcgccc cggatacaac ttataacggt
    14161 aagagtattg aacagatcaa cctgatgaaa ggaagaaagc acaaggccaa atacgaagcc
    14221 gaaacggtaa ttgatattat gatggccggt ggcgccagcg ccgtttttca tggcatgagt
    14281 gaagatgata tcaaacgtat catgcaatac ccgttcaaca tgtttgccag cgatgccacg
    14341 atacgagtgc tgaatgccgg tatgccccac ccgagaggat atggcactaa tgcaagggtt
    14401 ttgtccaaat atgtacggga agagaaagta atatcgcttg aagaggccat ccgccgcatg
    14461 acatcattac cggcacaaaa atttcaactg aaagaccgcg gcctgctgaa agaaggcatg
    14521 gcggctgaca ttgtcatctt tgatgaaaac gaagtgaaag acgtttccac ttttgaaaag
    14581 ccccatgctt actccaaagg ctttcatttt gtaatcgtca acggagtgct tacggtcaac
    14641 tatgaaaaac accttggcgt aagggcagga aaagcattat acgggccggg gacagtgaag
    14701 taatcttata ccgataacac ttcttttgta ttcgctttcc gcatcggttg ctttttccaa
    14761 taggtcaaac ttcgccagca ccattccagc ggaccaaaac gaaagaagcg cagccatagg
    14821 tggctccaga ttatttctat cacccatacc gcagccacta cataataaat ttcatagcgc
    14881 tgtaatttgc ccatcatacc taaaccaaca ccgaagaaga ataaaccaca cattaatgat
    14941 tgcatcaggt agttagtaaa tgccatttgg cctaccggcc gcatcaaagc aaagagccat
    15001 ttgaaccagc ctgatttata cagtaacatg atcaatccaa ataagcctaa tgaacgaagc
    15061 gttcttgaaa tctcataaaa ttcaaactgt ttatccttgg caatgttaaa ctggttgaat
    15121 tgatgatcta ccataaactg caacctgtac catgtcaaca acaatcccga acccatacct
    15181 acgataaaca aaacccagta taattttgca ggcgcctgcc cggtaagaat acctgatttg
    15241 aaaaaggcca tacctaacaa cataaacaag agtatatccc atatgccata atatgtataa
    15301 taaaactcca gctttacgct tatgttgctc atatattcat atagcgtact ataactaccg
    15361 gtagctttct gcaggttttt tctcatttca ttacgaagcc cggaagtatc cgatttttct
    15421 ttcataccgg tcatggcttc cagttgttcc tgctgcaggt ctgtcagctt tactttggtg
    15481 gtgtctattt ttgccgccat ttcacctttg taaattttgt ccttctggcg atgcaggtca
    15541 atattctccc gggtggtcat cagcaaaagg catacagctg cagctattaa caaccctttt
    15601 accgctaatc tccgaaatac aaatgccacc acaccaataa tggaatactg aaaaagaata
    15661 tcacccggcc ataagagaat aaatgcgttg accagtccga agacaagcaa ccacaactgc
    15721 ctgcggataa aatattctgc cggccatata ccttcggttc tcttttcaag tcttgctaca
    15781 aacagaataa taccagcgcc aaacaacata gaaaaaatgg cccgctgcgt tccctctggt
    15841 atccagttca cataccacca tactttttca ttgatggtgc ccatctcata attcaaggtg
    15901 ggattatcaa atgatggttc cggcaaaccc atgtaaggga tattcatgag taaaatacca
    15961 agcagggcca ggccccttaa agaatcaagt aaaacaatcc tctctccctg gctaacgggt
    16021 gcagccagag ttgcagcggg ttggttggac atggtatgtt ggttttggtt ttatcccgtt
    16081 tctacacgac ctgcttcagc agctctctat gacttcttac cacatgtggt ttgaattttt
    16141 cataccactg ctgccattcg tctttccgca tactgcccgt cagtgattgc tcatactccg
    16201 tcagcgtttc aaacccctcc tcgaagatga gccggtaggc atcaccggtg aaatcggaaa
    16261 gtactcttgc attcttgtta tccggtaata agccgccttt cagggcttcg tcgagcaatt
    16321 gtttggcggg gcggtagtgg ccaaactgca ggtaaaagat gtctcttaca atgtacatac
    16381 accaggtttt agtagtgaat aaatattgtc aacctggtac ggagaaaacc aatggatcag
    16441 taaatgagta ttaaggtaag gtatttcgac ggaaaaatta tttactacta ataagacatt
    16501 aatttttgaa tagtttcatt cagtctcgat ttggccagaa aattttgttc taattcaatg
    16561 gagaaaggaa ctggtgtatc cagcgaagca cagcgcatca ccggtgcatc caataattca
    16621 aaacagtttt ctgctatcca tgcggctatc tcaccaccga taccaccaat gagtgtgtct
    16681 tcatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnncaatag aaatttccgg atgctcggcc gcatagtctt
    16801 ctgcccaatg tacgccgctt ccataagtga ttatagaaac ctcatctcct tctcttacat
    16861 gcctggcttt cccgatctct attgcataat attcctcggg aacagggcca ctcaccgaac
    16921 gatacaaggc cttgtgctca aaatacatca ccgggttcgg atcattaatg gcagcaatga
    16981 gcaagccttt tgcatccatt ggtgtagatg gatacaccac tttcaatccc ggcactttgg
    17041 taaaccaggc ttcattgctc tgtgagtgaa aaggaccggc cccaacacca ccgcctgccg
    17101 gcatccttat tactacatct gcattttgtc cccagcggta atgaattttg gcgaggttgt
    17161 ttacaatctg gttgaagccc actgtaacaa aatcagcaaa ctgcatttcc atcatactct
    17221 tgtatccttc taatgataaa cccagtgcag tacccacaat ggcactttca caaagcggcg
    17281 tattcctcac tcttgctttg ccgaattcct gtacaaatcc ttctgtgatc ttaaaagctc
    17341 cgccatactc tgcaatatcc tgccccatga ggatgagatt gggatgttgt tgcatggact
    17401 gatggaggcc ttctttaata gcatcgatta gccgggaatc ttttgtcggg agccgggagt
    17461 caaaaccagg tcttgaatta tccactacat gtgccttgct gattgagcct tgtacctttg
    17521 catacacatc ctccagttct tcttccgtat cagccactac cggttttgta ttaaatccaa
    17581 tggcgagttc gctttctatt ttctctttga actcatttct gatctcggcc actttcattt
    17641 cactgagcac tcctgtatta aataagtaat gctcataatt tttgatcggg tctttttgtt
    17701 cccagagacg gaacagatcc tgaggtacat acttggttcc gctggcttct tcatgtcccc
    17761 gcatacggaa agtcatacac tcgatgaggt agggcttttg gtgtttgata cagaattccc
    17821 ttacaccttt tatggtgtca taaacagcca acaaattatt tccatcaatc tgcacacctt
    17881 ccataccata tcctttggct ttatcaacca aactgatgca gcggtattgt tcatttaccg
    17941 gtgtgcttaa cccgtagcca ttattttcaa ttaaaaagat aaccggaagg tcccaaacgg
    18001 ctgctacatt caatgcttca tgaaaatcac cttcactggt ggcaccgtcg ccagtgaaag
    18061 ccagtgtgac cttatcatcc tttttgagtt tgtaagctaa tgctacccca tccgcaatgg
    18121 ccatctgcgg accgagatgt gagatcatac cacagatatg atgttcacgg ctgccgaaat
    18181 gaaaacttct ttctcttgct ttgctgtagc cttctttggc tccctgccat tgcataaaca
    18241 agcggcttaa cggcatgttg cgagtagtaa acacaccgag attacggtgc atcggcataa
    18301 tccattcatc ttcctgcaac gccagggttg caccgacagc aatggcttcc tggccgatac
    18361 cactgaacca tttggatatt tttccctgcc tcagcagcac cagcattttt tcttcgatca
    18421 tgcggggcca cagaatgctg cggtaaaaat gggtcaatgc ttcatcagtc aaaccttttc
    18481 tttcaaagta catgctgcaa acctaattaa gttgaacgat accaaatttt tatacccccc
    18541 cctgtaagct actgatacaa tttcatatta atatcaaccg ccgtaatcac ccgttcattc
    18601 acacctgttg ataaatttca ccatttgaaa ttttgctatt gcaaatatcc ataagtattt
    18661 ttacttagtt aaggctaagt aatatctatg agaaaagttt ttacccttgt tcaattgttg
    18721 tgtttttttt ttgcttcagc tcaggacctg aactggaata cagtttccta cactaccggc
    18781 tcactgtctg ctaattttgg tttgattggc agtccggcat caaccgtaac atttaccgtt
    18841 acaggaaaca ctaacaggat tgatgcaaat tatcctgtaa agtacatagc aaatccttcc
    18901 ggcagtgcca atgattgcgc tgtaaactgt gcggtccgct cgtcagttac tttcactacc
    18961 ctttctgagt ctgttgttta tgaatttaat ttttctcctg ctgtaacggg tctctccttc
    19021 cgtatttatg atattgatgg tacgaatgcc tccagcggag accgggccat tgttacggca
    19081 gttaatggaa ctacggcaca aaacattacc atgactacaa gttcaggtcc aacaataacc
    19141 ggcagcggaa ccaccactgc cactgcaaca ggcactcagg gcaatactac agatgatttt
    19201 ataaacgtca gcatcatcag cggggttacc cggctttctg ttgcttactc caacaatcct
    19261 aataacggat cacccggtaa ccgcagtttc tccattggca atatgagctg gtccggcgta
    19321 ttgccggtaa aatgggtttc ttttacaggc cgtaaacaaa tcaacggcag cgttttactg
    19381 aactgggtaa ccgcaagcga aaccaatgct gataaatata ttattgaaaa aagcatggac
    19441 gggcaacgct ttaatcctgt tagcgaatta cccgccaccg gtagaaacaa catcaattat
    19501 tactctttta acgatgcagg ccccattgcc ggcaccgttc tttaccggat aaagcaaaca
    19561 gattttgacg gcaaatatga ttactcgggt attgtaatga taaagcaaca ggcaggtttg
    19621 caaaagagtt tagttttccc caaccccgca acagacatgc tgactattac gcttccggga
    19681 aatgtgcaat taaaaaatgt aaaagttttt gatgtgaacg gacgccttgt tatggatgcg
    19741 gtaaatacaa gcaacctgtt aaacattaag agactcagca agggcttgta cagcctgcag
    19801 gctgaaaata cctccggtga aattttcagc agctatttta ttaagcaata gcttttatat
    19861 aacaaccagc tcataaaact cctccggctt aaggtatttc tgtgccaaaa actgcaactc
    19921 ttcggctgag atggttttga tggtatgaat agaatcgtag aaatattgtt cggtgagatt
    19981 gtttaaaacg atattcttcc agcggcccat gatctggaaa ggcccgtcca ggtcgccgag
    20041 aatgcccccc atcatataat ttctgacaag cagcaattct tcttcatcca caatttgttc
    20101 ccgcagcaac ttcatttctt tgtacacctc ttcaatggtt gcttcacaaa catctttacc
    20161 ggcttcggta ctgatcatcc acgccgtatc atgaatatgg ttttgaataa aactatgaat
    20221 accgtaggta tatccttttt cttcccggat attactcatt aaccgtgaac caaagaaacc
    20281 accaaacaat gtattcagca ccatcacttt cataaaatca ggatggtgac ggttaggaaa
    20341 tgaacgggcc atgcgaacag aaccctgcac accggcctca tcgttagtga tccggtattt
    20401 tctgtctgca gctggctgtg ccattatctc ctgtaaagca accggctgat taggcaggtc
    20461 gccaaaatac tgatccagca aagaagcaat atcggcaggc agtttacctg ccacaaacag
    20521 caccagcttt ccctgctggt aatacttttt ataaaaatca agtaactgct ccctgttcaa
    20581 tgtatcaaaa tcttcaagct tgctgtattt accataggga tgattttcgc catacaggta
    20641 aacatcaatc aggcggctcg ccacaaaatc actttttttc aagttcacct tcagccgctg
    20701 cttcatgttc tgtttataga tatcgagttc ttcctgcggc attaccgaat caacaataag
    20761 ttccctgaca gaaggcaata attcttttat gtgctttgaa agacagtgaa gcgtaatagt
    20821 agctgtttcg ttgtagcagg ccctgttcag gtaagagcca tagtattcaa aatactcatt
    20881 gatctggaac gctgttttct ttgatgtacc attccgcagt aaaaagttag tcgtggccgc
    20941 aaccaggttt tgctcctcaa accagttacc ggcataaaat acccattcta cggccagcac
    21001 ttcttcagcg cctgcatcca cggcatatac ttctacacca ttctttagca caaacttttc
    21061 tgcctgcttc agctgcaatt taaaatttac tgcatccaca atttccgggg catgtttcct
    21121 gtctggcata aattaattcc cttcgtttat ttcttcaatc agttttgtga taagtaatgg
    21181 atcgtactgc agttttccct gcggaagatc tggcggcttt gttccaatat atcttctgta
    21241 gtcactttcg catatttctg caattcaaaa ttcatccagg ccgcatcgcc cagtgtttca
    21301 taataggcca ggctgttggc cctgctcatc acactcatgt cttcaaaagc gatcatgctt
    21361 tctgttttgt tctttacttt ctgcaattcg gcatctgcta ctttttcatt cttcattttc
    21421 tccagttcct tttccactgc cttttctgcg tcttccatct tcactccttt caccagtttg
    21481 ccctctacca ccacaaggcc ggcatcggta ctgccgaaat gatgacattc aatattgctg
    21541 aacaattttt gctcttttac cagtgattga tatagccttg atgaaccgcc accgctgagg
    21601 atgtccgtca tcaggtcaac agcataataa ctgctgtcca tcctgctgcc catatgccag
    21661 catttatata aggcatccag cggcacattg gcttttattt ccaaaaaccg ttcttcggtt
    21721 tgttcgggct ccaccggcag gctgcggtta tatttttctc cggaaggaat atctccaaac
    21781 catttttggg ccagctcttt cactctttcc actgtaacat ttccggctac taccagtaca
    21841 gcattcacgg gacggtaatg tttgaagaaa aagtttttta cgtcatccag ttttgcattc
    21901 tctatatgcg atagttcttt accaatcgtc atccagcgat acggatgttt tttataagcc
    21961 agttcccgca tcttatgcca cacatcacca tagggcttgg tgaggtagtg ttctttaaat
    22021 tcctcacaca ctactttgcg ctgcacatcc aggctttttt cgctgaaagc cagtgatagc
    22081 atccggtcac tctccagcca gaaggcagtt tccaggtttt cagccggcag ttgaatataa
    22141 taattggtga gatcgttggt agtatatgca ttattctccc caccggccat ttgcagcggc
    22201 tcatcatagc tgggtatatt ttctgaaccg ccaaacatca ggtgctcaaa aagatgtgca
    22261 aaccctgtcc tttcgggatc ttcatccctt gcgcccacat catacataat attcattact
    22321 gccatcggcg tggacaggtc ctggtgtaca attacccgca gaccattggc cagtgtaaat
    22381 ttttcaaatt ggatcataca gttctaacag ctttaaacaa aacaagttgt aaaagtattg
    22441 aaaaatacgg ctcgggacgg tgccgttgtc agagaatgct ttttacttta aactctactt
    22501 ccatcaatct gctatcacga cggaagacaa tcttgacctt ttcaccgggt acctgcaaag
    22561 caatcttgta ttgattcagg ttttgcgaaa aattcttgtt gattgcaatt acttcatccc
    22621 cttctttcag cccggctgcc tcacccgggg aacctttggc cacatcgccg ataataataa
    22681 gcccattaat aaagtaaagc tctattccgg aataggaata atcaaaggtt tcattgaagt
    22741 gccggttagg aatgataaat atgtcacctt tggcatagtt gagaataata ttgaaccgcc
    22801 gtaaaatatc attgccaatc aggccgccca tgtaaggata gctggttaca ttattctcat
    22861 catcaaaaat aaaagtggga acgttcctga atttatacgg gccaattttt acttcccgca
    22921 tcaacatcag ctccatatca atttttccgc ccagtccctc accttctttg ataaatcttt
    22981 ttttcttctt gctcaggaaa ttactgtctt ctgcaaattc tttggacaac agcatacaaa
    23041 gccctgcacc catatcatac agaaaacggg aatagatcgt tttttcatcc cgcacccgca
    23101 gcggttgaga tactaactgg ttgatcgttg gcttcagcag atagccgcct ctcgggtaac
    23161 ggattgttcc tttggtacaa aaactgatcc gcatactgtc gtaatctact ttcaaaatgt
    23221 agcggctcaa tacagaatat ccaatgatac cgtcaatacg ttcgccgtaa acagctgtca
    23281 gtatggtgta atcattaatg tgaaaattaa gactgtcaac cgtcaacccg gggaaatgta
    23341 atttctggtt gtagaggaag ctcactttgc gaatgcccgc aataccgcgg atggttctgt
    23401 cggatggcgt tggcttcatt tttaaataat ccaccgtagt agaatccagt gaaataccgc
    23461 tgctgccggt atcaagaata aaattgaggg tgtccggaaa actctccagc ttggcctgca
    23521 tgataacaat gccgccggta agctgggtaa acggcacttt ggtaatttcc cgggaaggtg
    23581 gttcaataaa ctcttcctgt gctttcagcg aattacctgc cagcaacatg ataccggtaa
    23641 aaaacagata tatggccttt tttcgcaaac tcatgctctc tttagacagg tatttgcctc
    23701 aaaatgatgc aacccggttt tttgtttatc gaaaattagt tgaatatatc agccttttac
    23761 aaccacacgt catataatct gttatagcct tgtacctttg cgcctatgca gttaaacgag
    23821 ttatacgaca aagccgctgc ctttggcttt ctgtctgttg aggaaggagt tcatctctat
    23881 catcatgcgc cactggccga tttgatgttc attgccgatg agctacggaa aaaacaggtg
    23941 ccgcatggaa aagtaacctg gcagatagac cgcaatgtga ataccaccaa tgtgtgtatt
    24001 gccaattgta aattctgcaa tttctatcgc atacccggac atgccgaagc ctacatcacc
    24061 gacatgccca cctaccggca aaagatcacc gagactttga aatacggcgg cgatcagttg
    24121 ttgttgcagg gcggtcatca tccggagctg ggtcttcaat tttatgtgga cactttccgg
    24181 caaataaaag ctgagtttcc caacatcaaa ctgcatgcac tgggaccacc cgaggtggcc
    24241 catatcacca aactggaaaa atcaacccac cacgaagtgc tgaaggcgct gaaagaagcc
    24301 ggtatggatt cattacccgg tgcaggcgct gaaattttga ttgaccgggt acgccggctg
    24361 atcagcaaag gaaaatgcgg cgcacaggaa tggctcgaca tcatgcatga agcacataag
    24421 ctgaatatta ctacttctgc cacgatgatg tttggtcatg tggagacaat tgaagaacgg
    24481 tttgaacacc tggtaaagat cagggaagta cagagccgta aacctgaaca tgccaacggg
    24541 tttttagctt ttattccatg gaccttccag gatgtggata cgctgctggc aaaaataaga
    24601 ggcgtacaaa atttaactac tgctgaagaa tatatccgca tgattgccct cagccgcatc
    24661 atgctgccga atgtaaagaa tatacaggcc agctggttga ccgtgggcaa gcagacggca
    24721 caaatttgtt tgcatgccgg cgccaatgat tttggttcta tcatgattga agagaatgtg
    24781 gtaagtgcag caggcgcccc gcaccgcttt acataccgga gtatgcaaga agccataagg
    24841 gaagccgggt ttgaaccaca gctgcgtaac cagcagtatg agtggcggga aattccggaa
    24901 acgatcgaag agcaaatgat agactattag tacccccttc tttaattcat gcttcgacag
    24961 gctcagcatg acagttcgtt tgaaatcgtt gtcaccctga gcttgtcaaa ggatgattat
    25021 tgtaaactac ttttaagaaa tgaaaagaac attcacctgg tttagaaaaa cagcatttgc
    25081 cgaaggcatt tcttttattg tcttattgtt tgtagccatg ccgctgaaat attttgccgg
    25141 tatgcccatg gccgttacga ttattggcag tgcacacggc gctttgtttg tggcttttat
    25201 ggcattggcc tatatggtaa aggaacagta caacaaatct ctgggctggg gagtgaaagc
    25261 ttttctggct tctattattc ctttcggtac tttttatatg gagaagcagt ggaagaagga
    25321 agaagcggaa ggggccgctg gttgattatg ctggtacaga ttgccattga ttttcacaag
    25381 aaagctgtgc taataatctc atagatttat gtgttagtgg taatattttg ataccacctg
    25441 atatatcaaa gagaacttaa ttatatttta tgacaaaata cattctaatc attttgcttt
    25501 taattagtcc attcattgga caggcacagg ctgaaaatca aaatcttccg aatgataact
    25561 taatgaaatc gtttacagat tcggctgttc aaaaatctgt attacctttt atgagtgaca
    25621 gcttaagggt tggactctcg attggaattt atcaaaataa taaagtattt acttacaact
    25681 acggctcaac tcaaaaagga aaacaaatat tgccaacaaa taatacaatt tatgaaattg
    25741 gttcaatatc caaaacattt acaggaacac tgcttgcgca agctgtgcag gacaaaaagg
    25801 ttaaaataga tgatgacatt cgtttgtatc ttgacaatag ttatccaaat ttagaatatc
    25861 gagggaaccc tatccgatta tcacatcttg taagccatat ttcaggacta ccattattcc
    25921 ttccggacat tcctactctt tttcagaatg ctaattttga tacattacct tttactattt
    25981 caaaaattca ggaaaactat tctaaggaga aattcttgga tgacttgcat aacgtaaaat
    26041 tggatacaat tccagggttt aagtttcgtt actcaaatgc aggtgctcag cttcttaaat
    26101 atattttgga aaaggtttat aaaatgcctt atgacaaact tttaaaaaag tatataagta
    26161 accctttgaa tatgataaac accaattctt tattttcaaa caataattta aacctcttag
    26221 taaaaggcta taatgacaaa ggaaacatta tgccttacaa tccacaaata cttgatgcag
    26281 caggtggcat tttttcaact gtttctgata tgttgaacta tatcagattt catttagatg
    26341 aaacaaatcc agtagtagca ttatctcaca aagccgtaaa agggaatatt aacgattatg
    26401 ccgtagggct caattggcag gaaatgacaa ctccaaaaaa acataagaaa atatggcaaa
    26461 gtggcgggac atttggattt tcatcttatt gtgtagttta tccagaactg aattttggaa
    26521 taatactttt gactaatgaa agcgatagaa tgacacaggg aagacttgaa gaggtagctg
    26581 acaaaatatt tgaaaaaata aatagccagt agccaaataa aaccactgct aacaagagta
    26641 ttaccaataa tggggatgac attggagcaa tagcagtagt aattctactg tactgtggtt
    26701 ccgggctcga cgaataaagc ccagcttaag ttcttaccat ttaactttat caaaaggcaa
    26761 ggcagcagtt ccggcgaacg gaatactaat tccccaccat cgccaatacg caaactgttg
    26821 ctacttgaat ctttcacagt agtattctac aactctctcc tgttaaagaa ttttaacgcc
    26881 ccttcttctt accctcattt atcctgactt caaaatacca tagctttatt tccaaacgaa
    26941 aagcttatga aaaatatatt ggtggcagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaaggg aaagggttac
    27121 ctgccctggg gcaattacag cgatattgtt ttttataaca gcgaaaccaa ccagtcgaaa
    27181 aaactatttg gcgataagct ggcattgatc aatacgttta gccagcgaag gaattattat
    27241 tactatgacg aaagcaggga aaaggaaata cctgcgaata ttctccgtca ccacattatt
    27301 tacctcgtcc gtacagaaaa cttcaacaat gatggcggcc tcgatactga tgaccctgtc
    27361 tacttgtaca tttccaccaa gacaggagat aacctgcggc agataacacc cggtggtttc
    27421 catgtgttgt cgtggactgt atccaaagac cagaaaatga tactggtaaa aggacagaat
    27481 gataagaacg gcaataaaaa atttggccag ggtgatgatc agctatatta ccggatagac
    27541 ctggaagatg atgtttcaaa gatcaggtgc tacccgttaa atttttaggg gcaacgtcag
    27601 tttaccagca tctgcttgtt atacttattc cggtattcaa ccggtgtgag cccggtaatt
    27661 ttcctaaaga tggtacgaaa agcttttgta tctgtataac ccacatccag cattacttcg
    27721 ctgatatttt tacggcttgt ttcaaaactt cgcttggcag cttccatttt tacccgttgt
    27781 atgtattcga ccacggtgtt gctggttgct ttcttaaacc ttcgctcaaa actgcggcgg
    27841 ccaatggcaa agtggcctgc cagctggtca acggataatt tatcgttata atgttcttca
    27901 atgaagtcct gtgccttttt tatctcttca tcttcatgtg tccgctgccc gttaaagagc
    27961 ataaaggctg cctggctgtc acggtctatt tctactgcaa aatacctggc agctaaaata
    28021 gctgtcgccc tgtcggtata tttttctacg aggtaaacca gcaggttcca gtaagaattg
    28081 gcaccaccac tggtatacag accctgctct tccgtaatga tacaactgtc taccagttct
    28141 gtccggggaa acatttttct gaactcattg ctgcggctcc aatgcgtaga gcatcgcttg
    28201 ccatcgagta aacccgttgc ggcaaaaaga aaagcaccga tacaaagtga tgctacttct
    28261 gctcctttat tatactgatg aatgatccag ggaatnnnnn nnnnnnnnnn nnnntgttca
    28321 atagctgccg tcatatcacc gctgagtgcg gggatgacaa taagatctgt ttgactgacc
    28381 tcctccggaa gcatatctgt atgtactgaa aaaatacttc cctccagttt tacctctttt
    28441 cttaatccta ctaactgtac attgaacaaa gcctcttttc cggcctcctg caaaaacata
    28501 ttgaccgcag aaaacatata acggggatca gccacggctt ctatcacggc ggtttcagga
    28561 acaagaatgg atacatgctt cattgaataa aggtaagaag ttggtatgtc gtaatcaacc
    28621 ccccagatag gcgtttttac acctcaattt attcaaaaag cctttccatc tttgtatggc
    28681 aaacagattt gttaagcaaa cctggtagaa acaatcaatc acctcaaaca aaaaccaaca
    28741 tggccagcgt aagtacttac ctgaactttc ctcagcatac agaggaagct ttccactttt
    28801 acaaatccgt ttttggaaca gaattcactc cgcccggtat ccgccggttt ggcgatatgc
    28861 cacccatgaa aggtgtgcct ccaacaccag atgctttaaa aaacctggtg atgcatgtgg
    28921 aactgcctat taccggcggg catatattaa tgggcaccga tgcaccggaa caaatgggat
    28981 ttacagtgag ctacggaaac aatattcata tcagcctcca tccggataca agaacagaaa
    29041 acaaacgttt atttgatgcg
//
LOCUS       DAORLS010000007        26945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_985_length_26945_cov_2.146337, whole genome shotgun sequence.
ACCESSION   DAORLS010000007 DAORLS010000000
VERSION     DAORLS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 26945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26945
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_26945_cov_2.146337"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK977_01220"
     CDS             <1..371
                     /locus_tag="PK977_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF16748.1"
                     /translation="YVLNALNTKSSEVVDARAKRWYDGDPTGNPRDGHITGAKNIPYP
                     DMIDSTNSIKPVSILEKNFSTVVPGKDKEVVVYCFIGQTACVDYLVGRSLGYKMKVYD
                     GSMQEWSRDTTLPMEKTIKQ"
     gene            443..790
                     /locus_tag="PK977_01225"
     CDS             443..790
                     /locus_tag="PK977_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRF16749.1"
                     /translation="MQRAISGYHLLMILTNVDGKLNVNEDLVIRDWLSQHFLVKPNLD
                     DEMEVISTLEKSDYASHFHTHMNLFYQHSEKQDRLELLQFAMNIIKADGQISKEENTF
                     FNELYDAWNEESE"
     gene            complement(842..1507)
                     /locus_tag="PK977_01230"
     CDS             complement(842..1507)
                     /locus_tag="PK977_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HRF16750.1"
                     /translation="MKRILLTCLSVSVILYACSNEKKKEEGNKDETKTESTETNNTST
                     APPDSAAQAEMMKAWQDFATPGPEHSWMNGHAGTWVCDSVAQWMDPAQPPTYSKATDV
                     VTVAMNGLYQMSDFSSNMMGMEMKGHGIMGFDKMKKKFVLSWIDNLGSGIVRMEGTYD
                     ESSKTLTMTGKQSDPGMKKETDMRQVMKFHEDDSYTMTMYGTGHDGKEAKFMEGAFKR
                     VKK"
     gene            complement(1606..1986)
                     /locus_tag="PK977_01235"
     CDS             complement(1606..1986)
                     /locus_tag="PK977_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRF16751.1"
                     /translation="MSEKKDIQNREDLLQLVTRFYEKLLGDDSISYLFTEIAKIDLQH
                     HLPVLVDFWDAILFQSDTYRKNAMQPHIDLHKQSALQKHHFETWLRYFKESVDELFEG
                     EKAFQAKERATSIATVMQIKISQL"
     gene            complement(1979..2896)
                     /locus_tag="PK977_01240"
     CDS             complement(1979..2896)
                     /locus_tag="PK977_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRF16752.1"
                     /translation="MILTVTLNPCIDKSSRVEKLKPESKLRCKEVVNEPGGGGINVSK
                     ALKKLDTSSVALFPAGGHNGNMLCSLLKEEEILFHAVDTKVETRENWIVLETSTNNQY
                     RFTFPGREVLEETIKSLVDQIRSFAPTYVVASGSLPPGLPDYFYGLIVKNAAAVGAKC
                     IVDTSGPALQALKGRKPYLIKPNIGELCKMLNVEWLDKDEVPDAAQQALRDGYAEIMV
                     VSMGPLGAWLITEEKRYFVEAPQVEKKSTVGAGDSMVAGITYSLQKGKTLREAIRFGV
                     ACGSAATMNDGTQLFNKADAEKLYAIINE"
     gene            complement(2904..3566)
                     /locus_tag="PK977_01245"
     CDS             complement(2904..3566)
                     /locus_tag="PK977_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRF16753.1"
                     /translation="MKPVVYYCYDAYCGWCYGFSPVIKRIASAYKDKLSFEVLSGGMI
                     PKEYERPISAIATYVSQAYRQVEEMTGIKFGEDYLWHIFNPDKSDWFQHSEKSAAALC
                     VFKEYHPDQQVAFAADLQYALHFEGRDLTDDEAYRHLTEKYSIQAVEFYQKLNSEEYK
                     EKAKYEFALCKQLQVTSFPQVLIRTGEQKFYLLAKGFTDYETLQARIEAVLHETQNNH
                     TK"
     assembly_gap    3630..3666
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3667..6484)
                     /gene="polA"
                     /locus_tag="PK977_01250"
     CDS             complement(<3667..6484)
                     /gene="polA"
                     /locus_tag="PK977_01250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRF16754.1"
                     /translation="MDKRIFLLDAFALVFRAYYALIRNPRLTSKGKNTNAQFGFTNAL
                     VELINKQKPSHMAVCFDTSAPTERHTDFADYKANRQETPEDILAAVPDIKKIIEGFNI
                     PIIALDGFEADDVIGTLAKQGEKAGYEVYMVTPDKDYGQLVSEKIKIYKPAYQGNDAE
                     IMGPEEVCAKWNIKEVSQVVDMLGMMGDAVDNIPGIPGVGEKTAAKFLAEYGSLENTL
                     ANVDKIKGAMGEKVKKGKKMAILSKKLATIITNVPVEFHEEDFRLKEWDKEKLKDVFA
                     ELEFRTLGKRLLGDDFSVAAPSKSVVEKEIPQGVQTDLFGNIIESPEPVTKSGEPATL
                     NNEETEEGLTVDKNIHNTPHNYQSVEGDAAIQKLVTALKKYEEICFDTETTGIDANDA
                     ELVGLSFSVKPGEAFYVPCPADQQRTKEILAFFEPLFSDSSKTWIGQNTKYDMLVLKW
                     YGIELAGNIFDTMLAHYVIEPDGKRSMDLLSEKFLGYEPVHIEELIGKKGKTQGNMRD
                     VEIEKIKEYAGEDADITLQLKNVFTPLLKQKEVERVFNEVENPLVKVLVDMEYEGIKI
                     DVAFLSDYSKELETEAKRAEESVYKQAGVRFNLASPKQLGEVLFDKLKLDPSAKKTKT
                     GQYQTGEDVLLKLAAKGNQIVDDILAFRELTKLKSTYVDALPQLINRKTGRVHTTYGQ
                     AVAVTGRLASNNPNLQNIPVRTDRGKEIRKAFIPRDDKHILLSADYSQIELRIVAAIS
                     GDLNMCSAFKAGTDIHTATAARVYNVPEQEVTKEMRYKAKSVNFGIIYGQGAFGLADN
                     LGISRTEAKEIIDNYKKEFPGIQKYMDDTINFAREHGYVQTLMGRKRWLRDINSANFT
                     VRGFAERNAINSPIQGTAADMIKLAMQKVHAAMKKEKMKSKMILQVHDELVFDALKSE
                     VKELKPLIIENMQAALPLPHDVPVI"
     gene            complement(6588..6833)
                     /locus_tag="PK977_01255"
     CDS             complement(6588..6833)
                     /locus_tag="PK977_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16755.1"
                     /translation="MKRRVRKIANFFAFNLLFFALYLNFIHKDESALSGMNQVATTQP
                     AAFSNTTVSVKNTEPQNTKAEQPVSKEENTALKLSIN"
     gene            complement(6986..>8777)
                     /gene="uvrB"
                     /locus_tag="PK977_01260"
     CDS             complement(6986..>8777)
                     /gene="uvrB"
                     /locus_tag="PK977_01260"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRF16756.1"
                     /translation="VEYFVSYYDYYQPEAYMPVSDVYIEKDLSINDELDKLRLRATSS
                     LLSGRRDIIVVASVSCIYGMGNPTDYENGIIRINKGQTISRQAFLHSLVNSLYNRTTI
                     EFNRGTFRVKGDTVDINLPYVDYGYRITFFGDEIEEIESIEVVTGKRIGKMENAAIFP
                     ANLYVAPKDILQQVLYEIQDEVTAQAEYFKKEGKYIEAQRLTERVNYDIEMIRELGYC
                     NGIENYSRFFDRRKPGTRPFCLLDYFPKDYLMVVDESHQTIPQVSGMYGGDRSRKLIL
                     VDYGFRLPSALDNRPLNFHEFESLQNQVIFVSATPDDYELEKCGGVVVEQVVRPTGLL
                     DPPIEIRPSVNQIDDLLDEIDKRVTKGDRVLVTTLTKRMAEEMDKYLHKINIKSKYIH
                     SEVDTLERVEILRELRLGKIDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRNEKSL
                     TQTAGRAARNVDGLVIFYADKMTESMQKTIDETTRRREKQVAYNIEHNITPRTVSKTT
                     QDVFAQTSVLDIKGYDPKDPYALAPDEELVTVAAEEQETYKTIPQMEKAISKIKKEME
                     KAARDLDFMEAARLRDEMFRLQKELETMKK"
     assembly_gap    8778..8923
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9178..9324
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9325..11624
                     /locus_tag="PK977_01265"
     CDS             <9325..11624
                     /locus_tag="PK977_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16757.1"
                     /translation="YIHYDKEYYVAGETIWFKAYLYNDGKPGGISSNLYVQLADINGQ
                     LIASGRYPVMGAVTKGSIVIPDSLPQGNYYIRAVTPHSLNGDEDFIYKKNLFIYRPSS
                     GTAVTKPQQTVALQFFPESGHLIDGILTVVGFKANDQWGTPLEAHGLIKTEDGTTVAP
                     FHSYHDGIGKVQFKPIAGKKYIAELETPAGKRTFSLPGVQTSGINLKIQDEKGGKKFQ
                     LSRGSKEKEKFETVLLVVQINNHVVNEFEITFEDYPSVIGHILTDSLPSGILHFTVFT
                     MDRLPVSERLSFVDNGEYKGHASIVPVTTSLQKRASNSLELSFSDSLQYSCSVSVVDI
                     SGGSFGDEENIWSRFLLTSDLKGYIHNPAWYFRQQNDSVKQALDNLMLTHGWSRFNWT
                     KLFAGQFPQHKYIDQDLIAISGKVVDERNKQPLSYGQLNVLLEAADSSQQTIELPVDE
                     KGRFARDSMYFAGKSKLFYGYLDKNGKQKPGIVEMDTDSLSYIINRVPKGLDGIAGMK
                     SPFNLLNKEEINNRFGYIKAENEEKVKELANVNIQAKSSKKPVDAVNEKYTTGVFRAP
                     GKVTLDNINEPANDRAMNVVDYIKNRIPQLTLQGGTFVNRKNFSLQSGRNWVVGIFLN
                     EIPTDINQLRILRADDVALVKFYEAGFVGVGSGSPGGALAVYTKEKSNKDLKPEKLDY
                     VEADGYAITKEFYSPDYSAPNPRHILTDNRTTLYWNPDVITDGETKVVKLNFYNNDFT
                     KKYKIVLEGFDATGRLVHVEKIIGE"
     gene            complement(11698..12240)
                     /locus_tag="PK977_01270"
     CDS             complement(11698..12240)
                     /locus_tag="PK977_01270"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF16758.1"
                     /translation="MKKTTFVLALVFAAGTVFAQKKTTSSATVTFDATTPKDALPKAE
                     NKTVIGSIDTQSGAVAFEAAVKNFAFSNPKIQEHFNAPNWLDSDKFPKFTYTGTIEKV
                     NKVKFDKNGTYKVQVNGTITVKGISKPAKVEGTITVADGKLKVAANFTIKLKDFGVTG
                     QAIEAGKVAEEPSITVSAEF"
     gene            complement(12257..13207)
                     /locus_tag="PK977_01275"
     CDS             complement(12257..13207)
                     /locus_tag="PK977_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16759.1"
                     /translation="MGSQPFISGTRYNEFMNNSATSNNTAKWLHLGSGLILLISYFLP
                     WVKWQATNVNGHDLAAGNFFQLSETNFKLGNPFPRFAFTFYIFWLIPLLVALTLFFVW
                     QNKKSAFPAFVAGALALSQITVYYLFTNTLVDLGVGKSALAMMQPAAFTTILAAVVFI
                     LSALPIGNWLKKSAWIIIGPVIAFASYKIGEKYVMAETFTSTNDIKEDYTVIANDLIR
                     EFISNDTAANNKYREKIIVVNGKTTQVERLSDSTTTIQFADSTGSYIAFSFEKEQYEE
                     VKEIKTGDQVSLKGSCSGSIYSEILGTTAITFKRSTINKK"
     gene            complement(13340..14344)
                     /gene="recA"
                     /locus_tag="PK977_01280"
     CDS             complement(13340..14344)
                     /gene="recA"
                     /locus_tag="PK977_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRF16760.1"
                     /translation="MSTNNEKLKALKLTMDKIEKDYGKGSVMMMNEKAEVQQEVISTG
                     SIGLDVALGIGGLPRGRVIEIYGPESSGKTTIATHVIAEAQKKGGMCAIIDAEHAFDS
                     SYAKKLGVDVDNLLISQPDYGEQALEIADRLILSGALDVVVIDSVAALVPKSELEGEM
                     GDSKMGLHARLMSQALRKLTATIAKTNTICIFINQLREKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRTQQVKDGDEAIGNHVKVKVVKNKVAPPFRAAEFDIIFGEGISKTGEII
                     DMGTELGIVQKSGSWYSYNNDKLGQGRDAVKQLLVDNPELAKEIETKIREKIKEMQQA
                     "
     assembly_gap    14703..14714
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14764..>15304
                     /locus_tag="PK977_01285"
     CDS             14764..>15304
                     /locus_tag="PK977_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF16761.1"
                     /translation="MFLYRMFYIFFLVPILCFGQVIKGLDLGGTSAPPKIVEGNTYAV
                     IVGINNYTSPKITKLNYAASDAIAFEQFLLSKAGGHVPKENIRRFLNDSATTGKFKKL
                     GLSWVKSKAKKGDRVYLYFSGHGITVSGGDSYFLAHNLASDFSVENIEEAGTQALYQL
                     KTNTIRPLCDSGVQVILIID"
     assembly_gap    15305..15314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15315..>16503
                     /locus_tag="PK977_01290"
     CDS             <15315..>16503
                     /locus_tag="PK977_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16762.1"
                     /translation="CRSPLAETLGSATVTTFNPDFNSAEMNAGDIMLLSSRNGQASLE
                     TDNDNIRHGVFTYYLLQGLYGMADNGKKADGLVTLNKLNNYVTKMVSDYTNESQIPLI
                     YHKGAQNPVIAIVDSNLLASHLKSLKQPGNELAVNTRKGNAKGFFTSKYSYLDTIGLY
                     DNFLTRLSEGKLWGDTGALYQFSRMEKFKFDIRLLNDAALSLSSVLVDSGQFIMNSYL
                     AADKKVFKNQGPASDYQFVKGAAYFDTLYKICSRYFPGDNDNKDYLQQYYFMKGRSLL
                     NAASRAEKEKALDLLNTAYSLDSSAYYILHVIDIFLEDLGLPEMALENQQRVIEKYPK
                     WTFAYNSLGNILYELKRYEEALAAYYKAIGLDSLYATPWIGIGNYYTDIVKDFNSAKN
                     YYL"
     assembly_gap    16504..16513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16514..>16894
                     /locus_tag="PK977_01295"
     CDS             <16514..>16894
                     /locus_tag="PK977_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF16763.1"
                     /translation="LSLDANYEVALGNLGNLFYQLKEYDSAMYFLQRAISINPRDDYN
                     LNKIGDVYKASREYDSAKYYYSKALEIDLAAENTMVKIGNIFYDGGEYDSATYYYKKA
                     IAANPKYEYAWANLGGVFKNSSKY"
     assembly_gap    16895..16952
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16953..>17292
                     /locus_tag="PK977_01300"
     CDS             <16953..>17292
                     /locus_tag="PK977_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF16764.1"
                     /translation="NEIGNLYNYYLDEYDSSIIYYHKAMALNDKEWVYPSNLGRAYLN
                     LGKLDSSAYYYWKAINLPNSNISHCFESLLDIYTNDTGDLADIEKIFIWLTTAPVSKG
                     SLWERLGDTYF"
     assembly_gap    17293..17319
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17320..>17842
                     /locus_tag="PK977_01305"
     CDS             <17320..>17842
                     /locus_tag="PK977_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16765.1"
                     /translation="EQCYQKAYEIDSADRYQYKIVDLKYAADPSQARIDLLAKVMGHE
                     NDVNKKLVEYGRYLNYAEDARLEYPDYNYPYNDSLITNFKKQGNNQQINSYLKTLVDF
                     DSLRAESWIKLAGEYKTYKLSKGSIIRFLKTSIRLDSAASEYEAWPELCSMQNSFVPT
                     AVAFLEKQKAKTTG"
     assembly_gap    17843..17948
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18106..18516)
                     /locus_tag="PK977_01310"
     CDS             complement(18106..18516)
                     /locus_tag="PK977_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16766.1"
                     /translation="MKHYHIVSRIAIYLLAVVLIAFGVFHFMYPRDLMVYVPLSLVGG
                     IKWAYVVGGAFILVGLSFLTNQFVKVTGYLLAILLLIFILTIHLPNYNNAGAEDMRRF
                     ALINILKDLAIMGFALHIAAGAHHQHIHVHEEDD"
     gene            18708..>18942
                     /locus_tag="PK977_01315"
     CDS             18708..>18942
                     /locus_tag="PK977_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16767.1"
                     /translation="MKKVLQMTFLLLFIVTGCGPSSKITSTWKADNAAPKKFKKIVVV
                     GIIRESDRTLRENMEQHLAADLRDLGYDATCSCD"
     assembly_gap    18943..18955
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18956..19392
                     /locus_tag="PK977_01320"
     CDS             <18956..19392
                     /locus_tag="PK977_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16768.1"
                     /translation="EYNPKAFENMTEEQAIGKMRNAGVDAVLTVVLLDKTKERYYVPG
                     RVRYTPYVVYHNRFWIYSRTVYDRIYTEGYYAENTKYFWETNLYDLTENGQQLIYSAQ
                     SQSFDPVSSETLGHEYGQMIAKDMVKNNVLMNLKTTTVMKAM"
     gene            19522..19731
                     /locus_tag="PK977_01325"
     CDS             19522..19731
                     /locus_tag="PK977_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRF16769.1"
                     /translation="MKNTSKMLIALGAGLAIGGVLGVLFAPDKGSETRKKISDQGKKL
                     ADKVKNKFHKEKEFEKVNGRVEELI"
     gene            19764..20132
                     /locus_tag="PK977_01330"
     CDS             19764..20132
                     /locus_tag="PK977_01330"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRF16770.1"
                     /translation="MSEEFNRIESLLGQAKEYVNTRIAQFKLSVAEKVAKIMATVIAG
                     FAAALVFFLFLVFAGIAAAIVLGQWTGYMWLGFLIMAVVYLLLAIIVWTAKDRLLALP
                     IMNAIIGRLFDEEEVEDEKD"
     gene            20119..20448
                     /locus_tag="PK977_01335"
     CDS             20119..20448
                     /locus_tag="PK977_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16771.1"
                     /translation="MKKIRRVQDMKQERMRLRIRQLELEKQLKISWKELKTGTKPFKE
                     PEEKKKESEEEPSRSLWSLALDYGTAYISQKLAAKAGQATEARLKESMDNLAEKLRTS
                     FSRKRKR"
     assembly_gap    20573..20587
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20588..20847
                     /gene="ytxJ"
                     /locus_tag="PK977_01340"
     CDS             <20588..20847
                     /gene="ytxJ"
                     /locus_tag="PK977_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRF16772.1"
                     /translation="HSTRCSVSSLIKTRLERSAIPSHIDFHYLDLIRYRSVSNKIAEE
                     FNVWHESPQVLLIRNGECVFDESHLAIYMEDIVAEAPAANN"
     gene            21048..21224
                     /locus_tag="PK977_01345"
     CDS             21048..21224
                     /locus_tag="PK977_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16773.1"
                     /translation="MKENWPYIFLVGVLSLAFIIFLIRQNRKDQKQLEKKLNEDYHKK
                     KDEENDVDTEDTPK"
     gene            21324..22364
                     /locus_tag="PK977_01350"
     CDS             21324..22364
                     /locus_tag="PK977_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRF16774.1"
                     /translation="MQQQPSFNNQIKQLMILILLLLLIFIALKELYIFLPGLLGALTL
                     YILSRGSYFQLVYHRKWRKGWAAGMFMLGFMVLLGLLIYFTVILLEKQVEPFFNNPAS
                     SLSQAKQAIVEVQEQAGIVLISDESLADLQHRISTFIPSLVNDTLNLLFNLAILLFVF
                     YYMLVHGKEMEAFLARIIPLKKTNIDVLASETKRLVKVSALGIPIISIIQGVTATIGY
                     IIFGVDEFVLWGFFTGVFAFFPVVGTMIIRVPLVIYMYVSGDTWNAMGLAIFSFVVTG
                     NIDYLARITLLKRMGNVHPLVTVIGVIVGLGLFGFIGLIFGPLLVNYIILLFRIYMNE
                     FIEPGEPETSDA"
     gene            complement(22361..22996)
                     /locus_tag="PK977_01355"
     CDS             complement(22361..22996)
                     /locus_tag="PK977_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRF16775.1"
                     /translation="MFFKNRFDAAVQLAPLLNKYKHQAAVIMAIPRGGVPIGYYLARY
                     LDLPLDLLMAKKLGHPANPEYAIGAVSLEGSIAEDCFDIPEKYILDETKRIREQLKLR
                     YKQFMGDKEPVDIKGRIVIVTDDGIATGRTILSVIKILRNKQPAKIVVAVPVSSEKAA
                     ERIRTVVDEFICLQTPYPFFAIGNFYEDFSQVEDDEVIQLLQELKAIDKVG"
     gene            complement(23007..23684)
                     /locus_tag="PK977_01360"
     CDS             complement(23007..23684)
                     /locus_tag="PK977_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF16776.1"
                     /translation="MQNILHRDITIPVDTIRLQAILTIPGKVMGIVIFSHGSGSSRFS
                     PRNRFVADILNQHHIATLLADLLTPEEDAVYANRFNIELLTERLIAITRWISQRDFGQ
                     TIPVGYFGASTGAASALKASVQCGNIVSAVVSRGGRPDLAAASLAFVKTPALLIVGSL
                     DEEVLALNRQALERLSCEKQLTIIEGATHLFEEPGKLEEVARLATDWFLHHLRQPAIM
                     DDIARNH"
     assembly_gap    24149..24218
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24219..24474
                     /locus_tag="PK977_01365"
     CDS             <24219..24474
                     /locus_tag="PK977_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16777.1"
                     /translation="GFNDSYSHGEYEDDLNPWHFQYRVIVRTVDEKGNVTEEKDMLAP
                     YNEKHLSNPNIRAMNNSYQIVLKNDEFKVISNSLVQKFSR"
     gene            complement(24489..25214)
                     /locus_tag="PK977_01370"
     CDS             complement(24489..25214)
                     /locus_tag="PK977_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRF16778.1"
                     /translation="MTTDLSWLSRTQLLIGEKALQQLATSHVMVVGLGGVGSYAAEFI
                     ARSGVGQMTIIDGDVVDPTNRNRQLPALATNHGVSKAMIMAERLKAINPELQLHIIKE
                     FINPEMVLQQLSHRPDYIIDAIDSITPKVTFIRLAYESKLPLVSSMGAGAKLDPTRLQ
                     VVDISKTYNCPFAQQIRKKLKEHKIYRGVKVVFSPEEPIKESLMLTDGKNYKKSAYGT
                     ISYLPAVFGAVASSVVIRDLIRK"
     gene            complement(25211..25864)
                     /locus_tag="PK977_01375"
     CDS             complement(25211..25864)
                     /locus_tag="PK977_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRF16779.1"
                     /translation="MYINMHSHNAPSPNEWMIQNLHDHWEKAVIPGRFSIGIHPWYIE
                     GTGQEQLRQLEQWSTHPHVLAIGECGLDKVCSTDFSLQQLVFTGQIRLANSIQKPLVI
                     HCVRAWEEVFSLLKKQGNTVPVIFHGFAKNAVMASRIISEGYYLSFGKALQQERVREC
                     LGTIPSDRFFLETDDAALSIETVYDLAASTLSIDHNSLSLQLQKNAAAVFGPAAVQL"
     gene            26091..26474
                     /locus_tag="PK977_01380"
     CDS             26091..26474
                     /locus_tag="PK977_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRF16780.1"
                     /translation="MATKKKPAKKAAKKPAAKKPAAKKPAKKAAKKPAAKKPAKKKSA
                     RKPNAAFMKALTPSADLAAVVGNKPLARTEAVKKIWAHIKANKLQDSKNRRMINNDAK
                     LKPIFGGKAQVSMFDVAKALAKHLK"
     assembly_gap    26572..26655
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26675..>26945
                     /locus_tag="PK977_01385"
     CDS             26675..>26945
                     /locus_tag="PK977_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16781.1"
                     /translation="MKDVSIIPRSEWKANDPIPYLRHEPVRITVHHEGTKLLVTDDAV
                     RKIKGIQTWGMGKDRNWADVPYHFFIAPDGKIYEGRNINTVGETNT"
BASE COUNT         7596 a         5405 c         5436 g         7773 t
ORIGIN      
        1 attatgtgtt gaatgcttta aatacaaaat cttcggaagt agttgatgca agggccaaaa
       61 gatggtatga tggtgaccca accggtaacc ccagggatgg acacattacc ggtgcaaaga
      121 atatccctta cccggatatg atagattcta ccaacagcat taaacctgtt tccatattgg
      181 aaaagaattt tagtactgta gtaccaggta aggataaaga agtagtggtt tattgtttta
      241 ttggtcaaac tgcctgcgta gattacctgg ttggccgtag cctggggtat aaaatgaaag
      301 tctatgatgg aagcatgcag gaatggagcc gagatacaac attgccaatg gaaaaaacga
      361 taaaacaata aaattacttt ttattattta agcaggaggt taaacaagaa gtacggatat
      421 ttgttgttaa aacaaaaaat tcatgcaaag ggcaatatcc ggctatcact tgctgatgat
      481 tcttaccaac gtagatggga aattaaatgt aaatgaagat cttgttatca gggactggct
      541 gagccagcat ttcttagtta aaccaaacct tgatgatgag atggaagtca tcagtacatt
      601 agaaaaaagt gactatgctt cacattttca tacgcatatg aacttgtttt atcagcactc
      661 tgaaaagcaa gatcgactgg aattactgca gtttgctatg aatatcatta aggccgatgg
      721 gcagatctca aaagaggaga atacattttt caacgaactt tatgatgcct ggaatgaaga
      781 atctgaataa aacaaaaaac ccggcactag cgccgggttt ttcttaaaaa aacgaaggtt
      841 attatttttt tactctctta aaagcccctt ccataaactt agcttctttt ccatcatgcc
      901 cggttccgta catggtcatg gtgtaactat catcttcatg aaacttcatt acttgcctca
      961 tatctgtttc cttcttcatt ccgggatctg attgcttacc agtcatggtc agtgttttgc
     1021 tgctttcatc gtaagttcct tccattctta caatgcctga acccaggtta tctatccagc
     1081 tcagtacaaa tttctttttc attttatcaa agcccataat gccatggcct ttcatttcca
     1141 tccccatcat attactggaa aagtctgaca tttggtacaa accgttcata gccacagtaa
     1201 cgacatccgt tgcctttgaa taagtaggcg gttgtgccgg atccatccac tgagctacgg
     1261 aatcgcagac ccaggtgcca gcatgaccat tcatccagct atgctccggg cccggtgtgg
     1321 caaaatcctg ccaggctttc atcatttcag cctgagcagc agaatcaggc ggtgctgtag
     1381 atgtgttgtt ggtttcagta gactcagttt tggtttcatc cttattccct tcctcttttt
     1441 tcttttcatt gctgcaggca taaagaataa ccgatactga taaacaggtt aaaagtattc
     1501 gcttcataaa aaaattttag aggcaaaagg tacaaaattg gcagacagtt tacaaagaag
     1561 agaaggagga atttaatttt aaaggataag ttaccaccgg ctaaattata actggctgat
     1621 ttttatctgc atgacagtag caatactggt tgccctttct ttagcctgga aagctttttc
     1681 tccttcgaac aactcatcaa cagactcttt gaaataccgg agccaggttt caaaatgatg
     1741 tttttgtaaa gcagattgtt tatgaagatc aatatgaggc tgcatggcat tctttcggta
     1801 agtatccgat tgaaaaagga tagcatccca gaaatcaacc agtactggta agtgatgttg
     1861 cagatcaatt ttggctatct ctgtaaataa ataactgatt gaatcgtcgc ccagcaactt
     1921 ttcgtaaaac cttgttacca gttgtaacaa gtcttccctg ttctgtatat cttttttttc
     1981 actcattgat gatggcgtat aatttttctg catctgcttt gttgaataac tgtgtgccat
     2041 cattcattgt tgcagcactt ccgcaggcca ctccaaaacg aatagcttcc cgtaatgtct
     2101 tacctttttg cagggaatag gttatgccgg ctaccatact gtcaccggca cctacggtac
     2161 tcttcttttc tacctggggg gcttcaacaa aatatctttt ttcttcagtg atcagccagg
     2221 ctcccaatgg ccccattgat accaccatga tctcagcgta gccatctctt aaagcttgtt
     2281 gcgctgcatc cggcacttca tctttatcca gccattctac attcagcatt ttacacagtt
     2341 caccgatatt cggctttatt aaataaggtt ttctaccttt taatgcctgt aaagccgggc
     2401 cgcttgtatc aacaatacat tttgctccta cagcagcagc attttttacg atcaatccat
     2461 aaaaataatc aggtaatccc ggaggcaaac tgccactggc aacaacatag gttggtgcaa
     2521 aagacctgat ttggtccacc agtgacttaa ttgtttcttc cagcacttct ctacccggaa
     2581 aagtaaaccg atactggtta ttggttgatg tttccagtac tatccagttt tcccttgttt
     2641 caacttttgt atctacggca tgaaacaata tttcttcttc ttttaataaa gaacaaagca
     2701 tgtttccatt atgaccgcct gccggaaata aggctacgga agaagtatcc agttttttta
     2761 atgccttact tacattgatg ccccctccgc ctggctcatt cacaacttcc ttacagcgaa
     2821 gtttactttc cggtttcagt ttttcaaccc tggaactttt atcgatacaa ggattgagtg
     2881 taacagtaag gatcatcagg taattatttt gtgtggttgt tttgtgtttc gtggagtacg
     2941 gcttctattc ttgcctgcag tgtttcgtaa tcagtaaacc cttttgccag taagtaaaat
     3001 ttttgttcac ctgtacgtat caatacctgc ggaaaactgg ttacctgtaa ttgtttacat
     3061 agtgcaaact catattttgc tttttctttg tattcttcgc tgttgagttt ctgataaaat
     3121 tcaactgcct gaatactata tttttctgtt agatgccggt aagcttcatc atcagtcaaa
     3181 tcccttcctt caaagtgtaa ggcatactga aggtccgcgg cgaaggctac ttgttggtca
     3241 gggtgatatt ctttaaaaac acaaagagcg gcggcagatt tttctgaatg ctggaaccag
     3301 tcacttttat ccgggttgaa gatatgccat agatagtctt ctccaaattt aataccggtc
     3361 atttcttcca cttgcctgta tgcctggctt acataagtag ctatggcact aattggtctt
     3421 tcatattctt tgggtatcat tccaccggat aatacttcaa aggaaagttt gtctttatat
     3481 gctgaagcaa ttcttttgat aaccgggcta aaaccataac accatccgca ataagcatca
     3541 taacaataat aaacaacggg tttcataagg ctgcaaactt atggatatga ggctgtataa
     3601 acttatgact tacgaaaaaa ctagtgagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnncaat aaccggtaca tcatgcggaa gcggcaacgc tgcctgcatg ttttcgatga
     3721 taagcggttt taattctttg acttctgatt ttagggcatc gaaaaccagt tcatcatgca
     3781 cctgcaatat catcttgctt ttcatttttt cctttttcat agctgcatgc actttttgca
     3841 tggccagttt tatcatatct gcggcggtac cctgtatagg agaattgatc gcatttcgct
     3901 ctgcaaagcc gcgaacggta aagttggcag agttgatgtc acgcaaccat ctttttctgc
     3961 ccatcagtgt ttgcacatag ccatgctccc gggcaaaatt gatagtgtca tccatatatt
     4021 tttgaatgcc cggaaactcc tttttatagt tatcaattat ttccttggct tctgttctgg
     4081 atatacccaa attatcggca agtccaaatg ctccctgccc gtatatgatg ccaaaattca
     4141 cactcttggc tttataacgc atctcttttg tcacttcctg ttccggtaca ttataaactc
     4201 tggcagctgt agctgtatgt atgtctgttc ctgctttaaa tgcactgcac atattcagat
     4261 caccactgat ggctgcaaca atgcgaagtt caatttgcga gtaatctgcg gatagcaaga
     4321 tgtgtttatc atctcttggt ataaatgctt tcctgatttc ttttcccctg tctgttctta
     4381 cgggtatatt ctgaaggtta ggattattgc tggccagcct gccggttacc gctacagcct
     4441 gcccataggt tgtatgcacc ctgcctgttt tccggttgat cagctgaggc agtgcatcta
     4501 cataagtaga tttgagcttg gtcagttcac ggaaggccag gatatcatct actatctggt
     4561 ttcccttagc agctaatttt aataaaacat cttcaccggt ctggtactga ccagtctttg
     4621 tttttttggc agaaggatca agctttagtt tatcaaacaa tacttcgccc aattgtttgg
     4681 gagaggcaag gttaaaacgt acaccagcct gtttgtacac actttcttct gcccgtttag
     4741 cttcggtttc cagttctttg gagtaatcac tcaaaaaggc aacatcaatt tttattcctt
     4801 catactccat atctaccagc actttcacca gtggattttc cacttcgtta aatactcgtt
     4861 ctacttcttt ttgtttaagc agcggagtaa atacattttt taattgtaaa gtgatatctg
     4921 cgtcttcccc ggcatattct tttatctttt caatctccac atcccgcata ttgccctgtg
     4981 tctttccttt ctttccaatc agttcttcga tatgtactgg ttcataacct aaaaattttt
     5041 cgcttaacag gtccatgctt ctttttccgt ccggctcaat aacgtaatgt gccagcatgg
     5101 tgtcaaaaat atttccggcc agttcaatgc cataccattt cagtacaagc atatcatact
     5161 tcgtattctg accaatccat gtctttgatg aatcggaaaa caatggttca aaaaatgcca
     5221 gaatttcttt tgttctttgc tgatcagcag ggcagggtac ataaaaggct tctcccggct
     5281 ttactgaaaa actaagcccg accagttctg cgtcgttggc atcaatgccg gttgtttctg
     5341 tatcaaaaca gatctcttca tattttttta atgctgttac cagtttttgg atagcagcat
     5401 ctccttcaac agactggtag ttatgaggag tattatgtat gtttttatca acagttaatc
     5461 cttcttcagt ttcttcatta tttaatgttg ccggctcacc agacttggtt actggttcag
     5521 gactctcaat aatgttacca aaaagatccg tttgaacacc ttgcggaatt tctttctcca
     5581 caacagattt tgacggagca gcgactgaaa aatcatcacc cagcaatctt ttacccaatg
     5641 ttctgaactc cagttcggca aaaacatctt ttaatttttc tttatcccat tctttcagcc
     5701 tgaagtcttc ttcatgaaac tcaacaggaa cattggtaat aatagtagcc agtttcttgg
     5761 aaaggatggc catttttttt cctttcttta ctttttcacc catggctcct tttattttat
     5821 caacgttggc caacgtattt tccagggaac catattctgc caaaaatttg gcagctgtct
     5881 tttctcccac acccgggata cccggtatat tgtctacagc atctcccatc atacccagca
     5941 tatctactac ctggcttact tctttaatat tccatttggc acaaacttct tcggggccca
     6001 ttatttcagc gtcattaccc tggtaagcag gcttgtaaat ctttattttt tctgaaacca
     6061 gttgtccata gtctttatcc ggcgtaacca tgtacacttc gtaaccggct ttttccccct
     6121 gtttggccaa tgtgccgatt acatcatccg cttcaaatcc atccaatgcg atgataggta
     6181 tattgaatcc ctcaattatt ttttttatat cgggtactgc agctagaatg tcttctggtg
     6241 tttcctgccg gttagctttg taatctgcaa aatctgtatg gcgttcagta ggggctgagg
     6301 tatcaaaaca cacagccatg tgtgatggct tttgcttatt gatcagttct accagtgcat
     6361 tggtaaatcc aaattgtgca ttggtgtttt tgcctttgga ggtaaggcgg ggattgcgga
     6421 tcagtgcata ataagccctg aaaacaagag cgaaagcatc cagcagaaaa attcttttat
     6481 ccatgcccct aaggtaaaga tttcagttgt aggcattgac aaaaacacaa cagcccctga
     6541 cataatcgtc aggggtgtta tttattaacg ctgctttgct tggtttctta gttgatagac
     6601 agcttcagtg ctgtattttc ttctttggaa acaggctgct ctgctttagt attttgtggt
     6661 tcagtgtttt taactgaaac agttgtatta ctaaaagctg ccggctgtgt agtagccacc
     6721 tggttcatac cgctcaacgc tgattcatct ttatgaatga aattcaggta taaagcgaag
     6781 aaaagtaaat tgaaggcaaa gaagttggcg atcttgcgga cacgtctttt cataaatact
     6841 ggatttacgg ttttactaat attggatttc gttcggtttt catagctaag atagagcggg
     6901 gttattctgg caacaaatcc ggaagaaaga aaatgtcaaa atcaggcaca agtactcagg
     6961 gtaaactcgt aaaactacga atcgcttatt tcttcatggt ttccaattcc ttctgcaacc
     7021 taaacatttc atccctcaat cttgctgctt ccataaaatc caaatccctc gctgcttttt
     7081 ccatttcctt tttgattttg gaaatggctt tctccatctg gggaatggtt ttatacgttt
     7141 cctgttcttc tgcagctact gttaccagtt cctcatctgg ggcaagtgca taaggatctt
     7201 tcgggtcata ccctttgata tctaatactg aagtttgtgc aaatacatcc tgtgttgtct
     7261 tggatacagt tctgggtgtg atgttgtgtt ctatattata agcaacctgt ttttcccggc
     7321 ggcgagttgt ttcatcaatc gttttttgca tactctccgt cattttatcc gcataaaaga
     7381 taacgagacc atctacattt cttgcggcac ggcctgctgt ttgagttaaa cttttttcat
     7441 tgcgtaaaaa gccttcttta tctgcatcta aaatagcaac aagtgatact tccggtaggt
     7501 ctaatccttc ccgcaggagg ttaacgccaa cgaggacatc aatttttccc aatcgaagtt
     7561 cacggagtat ttccactctt tccagtgtgt ccacttcact gtggatgtat ttggatttga
     7621 tattgatctt gtgcaggtat ttgtccatct cttcagccat ccttttggtc agtgtggtta
     7681 ccagcacccg gtccccctta gttacccgtt tatctatttc atccaacaag tcatcaattt
     7741 ggtttactga aggacgaatt tcaatcggcg ggtctagcaa gcctgttggt cgcaccacct
     7801 gttccactac cacaccacca catttctcca gctcataatc atctggtgtg gcagaaacaa
     7861 atatcacctg gttttgtaaa gattcaaatt catgaaaatt aagcggccga ttatccaatg
     7921 cactgggcaa acgaaaccca tagtcaacta atatcaattt acggcttcgg tcgccaccat
     7981 acataccgct tacctgtggt atcgtctggt gactttcatc tactaccatc aggtaatctt
     8041 tcggaaaata atccaacagg cagaaaggtc ttgtgcctgg ttttcgccgg tcaaagaagc
     8101 gggaatagtt ttcgatacca ttgcaataac ccaactcacg gatcatttca atatcataat
     8161 taacccgttc agttaatcgt tgtgcctcaa tatattttcc ttctttcttg aaatactcgg
     8221 cctgtgccgt tacttcatcc tgtatctcgt agagaacctg ctgtaaaata tcttttggcg
     8281 ctacatataa attggccgga aaaatggcgg cattttccat tttgcctatc cgcttgccgg
     8341 taaccacttc aatgctctct atttcttcaa tttcatcacc aaagaaagtg atgcgatagc
     8401 cataatctac ataaggcaga ttaatatcga ccgtatcgcc tttgacccga aaagtaccgc
     8461 ggttgaattc tattgtagtt ctgttatata aagaattaac caatgaatgt aaaaatgcct
     8521 gccgggaaat cgtttgccct ttgttgatgc gaatgatacc gttttcatag tcagttggat
     8581 tacccattcc ataaatacag ctcactgatg ccacaactat gatatccctt ctgccgctta
     8641 acaggctgga tgtggctctt agtcttaatt tatccagttc atcattaatg ctcaggtcct
     8701 tttcaatata tacatcactc acaggcatat aagcctccgg ctggtaataa tcatagtaag
     8761 aaacaaagta ttccactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagtgtc tggtattttt
     8941 cattattcag cacaccttcc gtcaactggc ggatagcttc cggctgatca cccgccggtg
     9001 gaaaaggagt atgaagcttg aatggcattg aacctgcaat ttaccaaatc agtaaaggaa
     9061 aaactaagga agattaacaa gggttaacat caaaatcatg caacaggcaa accggaaaaa
     9121 ctatctttat ccgcattctc ttccgttggt gtaactggaa gagaaaagga acattttnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnntttata tacactatga caaggaatat tatgtggcgg
     9361 gagaaacgat ctggttcaaa gcttatctct acaatgatgg aaagccggga ggtatcagca
     9421 gcaatttgta tgtgcagtta gcagatatca atggtcagct cattgcttca ggccggtacc
     9481 ctgttatggg ggctgtcaca aagggaagca ttgtaatacc cgattctctt ccgcagggta
     9541 attactatat aagagctgta acgcctcatt cgctaaacgg agatgaggac ttcatttata
     9601 aaaagaatct ttttatttac aggccatctt ccggcactgc tgtaacaaaa cctcagcaaa
     9661 ctgttgcctt acagttcttt ccagaaagcg gccacctcat tgatggaatt ctgacagttg
     9721 tgggatttaa agccaatgat cagtggggaa caccgttgga agcccatggg ttgataaaaa
     9781 cagaggatgg tacaacggtg gcgccctttc acagctacca cgatggcata ggtaaagtgc
     9841 agttcaagcc cattgcagga aaaaaatata ttgctgaact ggaaacgcct gccggcaaaa
     9901 ggacattttc tttaccagga gtacaaacat cagggatcaa tcttaaaatt caggatgaaa
     9961 aaggggggaa gaaatttcaa ctttcacggg gcagcaagga aaaagaaaag tttgagacag
    10021 ttttactcgt agtgcaaatt aataatcatg tggtgaatga attcgaaatt acattcgaag
    10081 actacccttc agtaatcggg catattttga cagacagcct cccttccggc attctgcatt
    10141 tcactgtatt tacgatggat cgtttacctg tttcggaaag actcagtttt gtagataacg
    10201 gggaatataa aggtcatgcc tccattgttc cggtaactac cagcctgcaa aaaagagcat
    10261 ccaattccct tgaactgagt ttttcggatt cattacagta cagttgttcc gtttctgttg
    10321 ttgatatttc aggcggaagt tttggtgatg aagagaatat ctggtcccgg tttctgttaa
    10381 ccagcgacct gaaaggatat attcataatc cggcctggta cttcaggcag caaaacgatt
    10441 ctgtaaagca ggctctcgat aatctaatgc tgacacatgg gtggagccgg ttcaactgga
    10501 ctaaattatt tgccgggcag tttcctcagc acaaatatat cgaccaggac ctcattgcga
    10561 tctccggtaa agtagtagat gagagaaata aacagccctt gtcatatggt caattgaatg
    10621 tattgcttga agcagccgat tcttcacagc aaacgataga attgcctgtt gatgaaaagg
    10681 gaagatttgc aagggattct atgtatttcg cagggaaaag caagttgttt tacggatatc
    10741 ttgataaaaa tggaaaacaa aaacccggta ttgtagaaat ggatacagat tcattaagct
    10801 atattattaa ccgggtacct aaaggtctcg atgggattgc aggaatgaaa agccctttca
    10861 atcttttaaa taaagaggaa ataaacaaca ggtttgggta cataaaagca gaaaacgagg
    10921 aaaaagtaaa ggaactggcg aatgtgaata ttcaggctaa gtcctccaaa aaacctgtgg
    10981 atgcagtaaa tgaaaaatat actactggtg tattcagggc gccgggaaag gtaacattgg
    11041 ataatataaa tgaaccggcc aatgacaggg ctatgaatgt ggtcgactat attaaaaaca
    11101 ggataccaca gctgacttta cagggaggaa catttgtgaa ccgtaaaaac tttagtcttc
    11161 agtcaggaag aaactgggtg gtcggtattt ttctgaatga aattccaacg gatattaatc
    11221 aactcaggat attaagggct gatgatgtgg ccctggttaa attttatgaa gcaggttttg
    11281 ttggtgtagg cagcggatca cccggtggtg ctttagctgt ttatacgaaa gagaaatcaa
    11341 ataaagatct gaagccagaa aagcttgatt atgtagaggc tgatggctat gcgattacaa
    11401 aggagtttta tagccctgat tatagtgcac ctaatccacg tcatatactt accgataaca
    11461 gaacaacctt gtactggaac ccggatgtga ttaccgatgg agagacaaaa gtggtaaaac
    11521 ttaattttta caataatgat tttactaaaa aatataaaat cgtattggaa ggctttgatg
    11581 caacaggtcg cctggtgcat gtagaaaaaa ttattgggga ataaggatac catagaacaa
    11641 agaaaagccc cttttcaaat ttttgaaaaa gggctttcct attataaaaa atgtttatta
    11701 aaactcagca gaaacggtaa tggacggctc ttctgctact ttacccgctt caatagcctg
    11761 tcctgttacg ccaaaatcct tcagtttgat agtgaagttg gctgccactt ttagcttacc
    11821 atcggctacg gtgattgtgc cttctacttt agcaggcttg cttataccct taacagttat
    11881 tgttccgttt acctgcactt tataagtacc gtttttgtca aactttactt tgtttacttt
    11941 ttcaatagtt ccggtatagg tgaatttggg aaacttatca gagtcgagcc agttgggggc
    12001 attaaagtgc tcctgtattt ttggattgct gaaagcaaaa ttcttcacag ctgcttcaaa
    12061 agctacggca ccggattggg tgtcaattga gccaataacc gttttgtttt cagctttggg
    12121 caatgcatct tttggtgtgg ttgcgtcaaa agttactgtc gcagatgaag tagttttctt
    12181 ttgggcgaat acagtgcctg ctgcaaaaac taaagccagt acgaaggttg tttttttcat
    12241 gtgtgatttt ttaggtttat tttttattta ttgttgatct tttgaatgta attgcggtgg
    12301 tgcccagtat ttcactgtat atgctgccgc tgcaggatcc tttaagagag acttgatcgc
    12361 ctgtcttaat ttcctttacc tcttcatatt gttccttttc aaaagagaaa gcaatatatg
    12421 agcctgtgga atcagcgaac tgaatagtgg ttgttgagtc acttaacctt tctacttgcg
    12481 ttgttttgcc gtttacaaca atgatctttt ccctgtattt attattcgcc gccgtatcat
    12541 ttgaaatgaa ttcacggata aggtcatttg cgattacagt ataatcttct tttatgtcgt
    12601 ttgtactggt gaacgtttca gccattacat atttttctcc tattttatac gacgcaaagg
    12661 caataaccgg gcctataata atccaggcag acttttttaa ccaattgcca atcggtaagg
    12721 ctgataatat aaaaacaaca gcagctaaaa ttgtagtgaa tgctgcaggc tgcatcatag
    12781 ccagggcaga tttacctact cccaaatcaa caagggtatt ggtgaataaa taataaacag
    12841 ttatctgtga taaggcaagt gctccggcaa caaatgcagg aaacgctgac ttcttattct
    12901 gccacacaaa gaataaagtg agtgccacca gcaatggaat caaccagaaa atatagaatg
    12961 tgaaggcaaa ccggggaaaa ggatttccca gtttgaaatt tgtttccgat agctgaaaaa
    13021 agttaccagc tgccagatcg tgtccgttaa catttgtcgc ctgccatttt acccatggta
    13081 agaaataact aatcagtaaa atcagccccg atcccaggtg taaccatttg gctgtattgt
    13141 tgctggttgc agaattattc atgaattcgt tataacgagt gccgcttata aatggttgcg
    13201 accccattaa acaaaaatac aatgtttaaa cattgaataa gcaaaagaac ataaaatatt
    13261 ttttgaagaa ataaaaagcc ccaacaaatc tgttggggct tttacttgct aacccattga
    13321 aaactaatca acaaaatgat tatgcttgct gcatttcctt gatcttttcc ctgatcttgg
    13381 tttctatttc tttagccagt tcaggattat ccaccagtaa ttgtttaacg gcgtctcttc
    13441 cctgtcccag cttatcatta ttatagctat accagctgcc gctcttttga acgatgccca
    13501 gttcggtgcc catatcaatg atttcaccgg ttttagaaat accttctccg aagatgatat
    13561 caaattcagc agcacggaaa ggaggggcca ctttgttttt taccactttt acttttacat
    13621 ggttgccaat ggcttcatct ccgtctttta cctgttgtgt acggcggata tccaaacgaa
    13681 ccgaagcata aaattttagg gcattaccac ccgtagtagt ttcaggattg ccaaacatca
    13741 ccccgatctt ctcccgcagc tggttgatga agatgcaaat agtattcgtc ttggcaatag
    13801 tggctgtcag cttgcgcaat gcctggctca tcaaccgtgc atggagtccc atcttgctgt
    13861 cgcccatttc gccttccagt tcgcttttcg gaacgagggc tgccacagag tcgatcacca
    13921 ctacatccaa tgcgcccgag aggatcaggc ggtcagcaat ttccagtgcc tgctcaccat
    13981 aatccggctg ggagatcagt aaattatcaa cgtctacacc taatttcttt gcgtaactgc
    14041 tgtcaaatgc atgttcagca tcaataatgg cacacatgcc gccttttttc tgtgcttccg
    14101 caattacatg tgtggcgatg gtggtttttc ccgaagactc gggaccatat atttcaataa
    14161 cccttcctct cggaaggccg ccgataccga gggccacatc caggccaata gaaccggtgg
    14221 aaattacttc ctgttgtact tcggcttttt cattcatcat catcacactg cctttgccgt
    14281 agtctttctc aatcttatcc attgtaagct tgagggcttt cagtttttca ttgttcgtac
    14341 tcatgtctgt agttttttca gtcattcttt gccatgcgtt aaaattaagt gtttgcgtat
    14401 tgtaggccga aaaattccgg ctgaacatca aagctaaatg atttagcaaa taaaagctaa
    14461 tattttgggg attattttaa gaaattaccc ggtttgtggt atttattcag aaggtaaaga
    14521 tattttctta accccttttc taaactaaat attagataaa atgcggcttg ctgaaaaaga
    14581 atattggtga aaggatgaag aaactgaaca atagtatgca aaagcttgct ttaagcaatg
    14641 agattaattc ggtggactgg agtttgtaat aaatagaaaa tcatacccta aaaagggtat
    14701 tgnnnnnnnn nnnngtattg gaatgataag tttatatctc gaaatttatc aaaattttgt
    14761 tcggtgtttt tatataggat gttttacata ttctttttgg tgcccattct atgctttggc
    14821 caggttatca agggtcttga tcttggcggc acttcagctc ctccgaaaat agtggagggg
    14881 aacacttatg cagttattgt cgggatcaat aattatactt ccccaaaaat taccaaactt
    14941 aattacgccg caagtgatgc gattgctttt gagcagtttt tattgtcaaa agccggcggg
    15001 catgtgccaa aggaaaatat ccgaaggttt ctgaatgatt cggctacgac cggaaaattt
    15061 aaaaaactgg gactttcctg ggtaaagagc aaagccaaaa agggggacag ggtttacctg
    15121 tatttttcag gtcatggtat taccgtcagc ggtggtgatt cctattttct ggctcataat
    15181 cttgcctccg atttttcggt agaaaatatt gaagaggcag gtacgcaggc cttataccag
    15241 ctaaaaacaa ataccatccg gccgctttgt gatagtggtg tacaggtaat tctcatcatt
    15301 gatgnnnnnn nnnncctgcc gctctcccct ggccgagaca ttgggaagtg ccaccgttac
    15361 cacttttaat cccgatttca actcggcgga aatgaatgcg ggggatatca tgttgctttc
    15421 atcccgaaac ggccaggcca gcctcgaaac cgataatgat aatatccggc atggagtatt
    15481 tacttattat ttgctgcagg ggctttacgg catggctgat aatggtaaaa aagcagatgg
    15541 gttggttaca ttgaacaagc tcaataatta tgtaactaaa atggtctctg attataccaa
    15601 tgaatcccaa attccattaa tataccataa gggagcacaa aatcccgtaa tagctattgt
    15661 ggacagtaat ttactggcca gccatttaaa aagtttaaaa caacccggta atgagctggc
    15721 tgtaaataca aggaaaggaa atgccaaagg attttttaca tcaaaatatt cttatctcga
    15781 tacaatcgga ctatatgata atttcttaac aaggctgagt gaaggaaagt tgtggggcga
    15841 taccggtgct ttgtatcaat tttcccggat ggaaaaattc aaatttgata tccgtttgct
    15901 taatgatgcg gcgcttagcc tctcttctgt gttggtagac tcgggacagt ttatcatgaa
    15961 ctcctacctg gctgccgata aaaaagtatt taaaaaccag gggccggctt ctgattacca
    16021 gtttgtaaaa ggggcagcat attttgatac cttgtataaa atttgttcga gatattttcc
    16081 tggtgataat gacaacaaag attacctgca gcagtattat tttatgaaag gaagatccct
    16141 gctgaatgct gcctccaggg cagaaaaaga aaaagcactg gatttactga atacagccta
    16201 tagccttgat tcctctgctt attatattct tcatgtcatt gatatatttt tagaagatct
    16261 gggattaccc gaaatggcac tcgaaaacca gcaaagggtg atagaaaaat accctaaatg
    16321 gacatttgcc tacaatagcc tgggtaatat actttatgaa cttaaaagat atgaagaggc
    16381 cctggcagcc tattataaag cgatcgggct ggacagttta tatgcaaccc cctggatagg
    16441 catcggtaat tattatactg acattgttaa agactttaac agcgcaaaaa attattattt
    16501 aaannnnnnn nnnccctttc ccttgatgcc aattatgagg tagccctggg aaatcttgga
    16561 aatctttttt atcaattaaa ggaatacgat tcagcgatgt attttctgca acgggctatt
    16621 tccataaatc cccgggacga ttataacctg aataagattg gagatgtgta caaagcttca
    16681 agagagtatg attcagcaaa atattattat tctaaagcgt tggagataga ccttgccgcc
    16741 gaaaatacaa tggttaagat tggcaatatt ttttatgacg ggggagagta tgattctgcc
    16801 acctattact ataaaaaagc gattgccgct aacccgaagt atgagtatgc ctgggcaaac
    16861 cttggtggtg tttttaaaaa ttcttctaag tatgnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatgaaat aggaaatttg tacaattatt
    16981 acctcgatga gtatgattcc agtattattt attatcacaa ggccatggct ttaaatgata
    17041 aggagtgggt ttacccttcc aacctgggca gggcctactt aaatctcggc aaacttgatt
    17101 catcagcata ttactattgg aaggcaatca atttgcccaa cagcaatatt tctcattgct
    17161 tcgaatcgct attggatata tatacaaatg atactggcga tttggctgat atagaaaaaa
    17221 tcttcatctg gctcacgacg gcacctgttt caaaaggctc tctctgggaa cgattggggg
    17281 atacttattt cgnnnnnnnn nnnnnnnnnn nnnnnnnnng aacaatgtta tcaaaaagct
    17341 tatgagatag attcggctga caggtatcaa tataaaattg ttgatctgaa gtatgcagca
    17401 gatccatctc aggcaaggat tgatctgctg gcaaaagtca tggggcatga aaatgatgtc
    17461 aataaaaaac ttgtggagta cggcaggtac ttgaattatg ctgaggatgc caggcttgaa
    17521 tatcctgact ataattaccc gtataatgac agcctgatca caaattttaa aaaacagggc
    17581 aataaccaac aaataaatag ttatttaaaa acattagttg atttcgacag cctgagggcg
    17641 gaaagctgga ttaagcttgc cggtgaatat aaaacttaca aattgtccaa aggcagtatt
    17701 atacggtttc tcaaaacctc catcaggctg gattctgccg ccagtgaata tgaagcatgg
    17761 ccggagctat gcagtatgca aaattccttt gttccaacgg ctgttgcatt tctcgaaaag
    17821 caaaaggcaa aaactacggg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnga ctataaagat caacagagaa gagttcataa aaatgcctgc ttctatgtgg
    18001 agtaagatat ggaggtgaaa aaaaataact gcagttctgc cataaaataa tagcataata
    18061 acagtcagcc ggctcagcca tcccccttat atccctcaaa aagtactaat catcttcctc
    18121 gtgtacatga atgtgttggt gatgggcgcc tgcagcaata tgcagggcaa agcccatgat
    18181 ggccaggtct ttcaaaatgt tgatcaaagc aaatcgcctc atatcttctg ccccggcatt
    18241 gttataattg ggcaaatgga tagtgagaat gaaaataagt aatagaatgg ccagtaagta
    18301 ccctgtaacc ttaacaaatt ggtttgtcaa aaaagacagg cccaccaaaa taaaagcacc
    18361 ccccaccaca taggcccact tgataccacc taccagggac agcggaacat ataccatcag
    18421 gtcccttggg tacatgaaat gaaaaacacc aaatgctatt aataccaccg ccagcaggta
    18481 aatagctatc cgtgatacaa tgtgataatg cttcatacag atagtttttg tgggtaaaag
    18541 gtagtttatt taaaaatcaa ttaatatgat tttacccccg gtcagctttt ttacaccaat
    18601 atccggcaga aaacaggaca ttttgaggct tgtcattctt ttcgctgagg gctgtcattg
    18661 ttttggctta aggtgaatac taccttacaa ctactaaacg gttaatcatg aaaaaggtat
    18721 tgcaaatgac attcttgttg ctgtttatag taacaggatg cgggccttct tccaaaataa
    18781 ccagcacctg gaaagcagat aatgcagcac ccaaaaaatt taagaaaatt gtggtggtgg
    18841 gtattataag ggagtccgac agaacattgc gggagaatat ggagcagcac ctggctgcag
    18901 acttgagaga cctgggctat gatgctacct gcagttgcga tgnnnnnnnn nnnnnatgag
    18961 tataatccga aagcctttga gaacatgacc gaagaacagg caattggcaa aatgcgcaat
    19021 gcaggagtgg atgcggtgct caccgtggta ctactggata aaacaaaaga aaggtattat
    19081 gtgcccggcc gtgtccgtta taccccgtat gttgtttatc acaaccggtt ctggatttat
    19141 agccggactg tctacgacag gatctataca gagggctatt atgcagagaa caccaagtat
    19201 ttctgggaaa ccaacctgta tgacctgaca gaaaacggac agcagctgat ttattccgca
    19261 caaagccagt ctttcgatcc tgtttcatca gaaacattgg gccatgagta cggacagatg
    19321 attgccaaag acatggtgaa gaacaatgtg ctgatgaacc tgaaaacaac cacggtgatg
    19381 aaagcaatgt gatgccctgg tgtgaaaaaa caagagtgac aagtatcatc tgttttgctg
    19441 aagaatctca taggaaatga gtatgtcaaa acagatattt gaaacgacag gttctaattg
    19501 ttcaccgtta aagaatgggt tatgaaaaat acatcaaaaa tgctcattgc attaggagcc
    19561 ggcctggcaa ttggaggtgt attaggagtg ttgtttgcac cagataaagg ttcagaaacc
    19621 aggaaaaaaa tttctgacca gggcaaaaaa ctggcagata aagtgaaaaa caaatttcac
    19681 aaagaaaaag agttcgaaaa agtgaatggc agggttgaag aactcatctg aacctttcaa
    19741 cagcctgcca aaagcaagtt atcatgagtg aggaatttaa caggatagaa tcattgctgg
    19801 ggcaggcaaa agagtatgtg aacaccagga tagcacaatt caagctttct gttgcagaga
    19861 aagtagctaa aataatggca acagtaatag cggggtttgc ggctgccctg gtgttctttt
    19921 tgtttttggt ctttgcgggt atagctgctg ctattgtatt aggacaatgg accggttata
    19981 tgtggctggg ctttttgatt atggcagttg tctacctgtt actggcaatc atagtatgga
    20041 cagcaaaaga tcgcttatta gctttaccta ttatgaatgc catcattggt cgtctctttg
    20101 atgaagagga ggtggaagat gaaaaagatt agaagggtac aggatatgaa gcaggaacga
    20161 atgcggctgc gtatccggca gctggaactg gagaagcagt taaaaataag ctggaaggaa
    20221 ctgaagaccg gaacaaagcc ttttaaagaa cctgaagaaa aaaagaaaga gtctgaagag
    20281 gaaccaagcc ggtcattatg gtctcttgca ctggattacg gaaccgccta catcagccag
    20341 aaactggcag ccaaagcagg acaggccaca gaagcccggt tgaaagaaag tatggacaat
    20401 cttgcagaaa aactcaggac cagtttttcc cgaaagagaa aacggtaacg gggttcacac
    20461 aaccattcct ttttattttt gtttttctta caagccgtta ttatgaattg gtccgtactt
    20521 gaatcagaag aacaattagc ccttataata gggcagtcgt acaacaggcc acnnnnnnnn
    20581 nnnnnnnaac actctacccg gtgctccgtc agcagcctta taaaaacaag gctggaaaga
    20641 tcagccattc cttcccatat cgattttcat tacctggacc tgatccggta ccggtctgtc
    20701 tccaacaaaa ttgcagagga attcaatgta tggcatgaat cgccacaggt gctgctcatc
    20761 cggaacggag aatgtgtatt tgatgaaagt cacctggcta tctatatgga agatattgta
    20821 gccgaagccc cggcagccaa taactaataa tgggttaagc ctcccttaca cacaatccgt
    20881 gagaaaacac caggaataaa cagcatggct cctgcaaaag gagtaaattc atagtgaaat
    20941 cagttacatg ccgcttagct atgttattta tggttctctt tctttagtag tgataacact
    21001 gatactgttt atattttcca aaaagaagga caaataaatc aaagacaatg aaagaaaact
    21061 ggccgtatat attcctggtg ggtgtgctgt cattggcatt tattattttt ctaatccggc
    21121 aaaacaggaa agaccaaaag cagctggaaa aaaagctgaa cgaagattat cacaagaaaa
    21181 aggacgagga aaatgatgtg gacacagagg acaccccaaa atagcaacta atcagtatca
    21241 ggattgggct ctgacatgta tcagcaaata attttaataa ttactgtagc cttgtattga
    21301 gatattattg gtaaaccgca gttatgcagc aacaacccag ttttaataac cagatcaagc
    21361 agctgatgat ccttattctg ctgttgctgc tgatctttat tgcactcaaa gaactgtata
    21421 tattcctgcc cggcctgtta ggtgctttga cattgtatat actgagccgg ggcagttatt
    21481 ttcaactggt ttatcaccgc aaatggcgca aaggatgggc tgcaggtatg ttcatgctcg
    21541 gattcatggt actgcttggc ttgcttattt acttcactgt cattttactg gaaaagcagg
    21601 tagaaccttt ttttaataac cctgcttcat cgcttagcca ggccaaacag gccatcgttg
    21661 aagtacagga gcaagccggt atcgtattga tttcagatga aagcctcgca gaccttcagc
    21721 atcgtatatc aacttttatt ccctcattgg tcaatgatac attaaaccta ttgttcaatc
    21781 ttgccatcct gttgtttgtg ttttattata tgctggtgca tggtaaagaa atggaagcgt
    21841 tcctggcaag gatcattccg cttaaaaaaa caaatattga tgtactggca tccgaaacca
    21901 agcggctggt aaaggtgagt gcattgggca ttcccattat ttcaattata cagggtgtca
    21961 cggccacaat cggttacatc atttttggtg tggatgaatt tgtattgtgg ggtttcttca
    22021 ccggtgtatt tgctttcttc ccggttgtgg gtaccatgat catccgggta ccactggtca
    22081 tttacatgta tgtcagcggt gatacctgga atgctatggg ccttgctatt tttagctttg
    22141 tggtaaccgg taatattgac tacctggccc gcattacctt attaaaaaga atgggcaatg
    22201 tgcatccttt ggttacagta attggggtaa ttgtggggtt gggcctgttt ggttttattg
    22261 gtttgatatt tggtcctctg ctggtgaact atatcatact tctgttccgc atttatatga
    22321 atgagtttat agaacccggg gagcccgaaa catccgatgc ctagcccact ttatctattg
    22381 cttttaactc ctgcaaaagc tgaatgacct cgtcgtcttc cacctgggag aagtcttcgt
    22441 agaaattacc aatggcaaaa aaagggtaag gggtttgcag gcaaatgaat tcatccacca
    22501 ctgttctgat tcgttctgct gccttttcgg aagaaacagg tactgccacc acaatttttg
    22561 ccggctgttt gttgcgcaaa attttgataa cacttaaaat agttcttccg gtagcaatgc
    22621 catcatctgt tacaataacg atccggccct ttatatcgac aggttctttg tctcccataa
    22681 attgtttgta gcggagcttt aactgctccc tgatgcgttt ggtttcatca aggatatatt
    22741 tttccggaat atcgaaacag tcttcggcaa tgcttccctc aaggcttaca gcacctatgg
    22801 catattcagg atttgcaggg tgacccagtt ttttggccat cagcaggtcg agcggaaggt
    22861 ccaggtaacg ggccaggtaa tacccaatcg gtacaccgcc tctcggaata gccattatta
    22921 cggcggcctg atgtttatac ttgttcagta aaggagccag ctgtacagct gcatcaaatc
    22981 ggtttttgaa aaacatgatc tttagtttaa tggttcctgg caatatcatc cataattgcc
    23041 ggttgccgga gatgatgcag gaaccagtct gtagccagcc tggcaacttc ttccagcttt
    23101 ccgggttctt caaacaagtg agtagcacct tctatgattg tcagttgttt ttcacaggat
    23161 aaacgctcca atgcctgccg gttcagtgcc agcacttctt catcaagcga accgacaata
    23221 agtagagcag gtgtttttac aaaagcaagg gaagctgcag caagatccgg gcggcctcct
    23281 cttgatacaa cagcgctgac aatattgccg cattgaacag atgcctttaa agcggaagca
    23341 gccccggtac tggcaccaaa atacccgaca ggaatcgttt gaccgaaatc tctttgggag
    23401 atccaccggg taatggcaat aagcctttcc gtcagcaatt caatgttgaa acggttggca
    23461 tatacagcgt cttcctccgg ggtgagcagg tcggcaagga gtgtggcgat atgatgctgg
    23521 tttaaaatat ctgcaacgaa gcggtttcgg ggactgaacc ggctgctgcc gctgccgtgc
    23581 gaaaaaataa caatacccat cacttttccg ggaatagtca ggatggcctg caaccggata
    23641 gtatcaacgg gtatggttat atctctatgc aggatatttt gcatagttta tccttttttc
    23701 aagccgtcat cattttcaat ttactgtgat caaacagcgt atttaactga gagggctcat
    23761 tcggcaggat gaaccttgtc attgggacaa tatttgaaaa cccggttcaa ctaacggcag
    23821 aagccgggat gccgcctgtt gattgtgagt tttatcaggc atagcagccc cttgcaggaa
    23881 taaatatcag ccggccggat aatctaaatc atccggcaca tccggatagt ttaacaactt
    23941 tggctcttaa tttttttaaa atttaaatat gaaatggcct ttccggatca ggcacatttc
    24001 atagaaacaa attcaaacaa taaaaacaag taaaatggaa aagcaatact ggatcaatgt
    24061 aaaacatgtt gacaacagaa tggtcatctt cctgaatggt gaagttatct gggatagcgg
    24121 tatcgttcag gacgaccctg aactggatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gcttcaatga ctcttattca
    24241 cacggggagt atgaagacga tcttaatccc tggcattttc aatacagggt aatagtgaga
    24301 acggtggatg aaaaaggaaa tgtaacagaa gaaaaagata tgctggctcc ttataatgaa
    24361 aagcacctgt ctaatcccaa tataagggcc atgaataaca gttaccagat cgtattgaag
    24421 aatgatgaat ttaaagtgat atccaattca ctggtacaaa agttcagcag gtaagaagaa
    24481 accaccattc acttccggat aaggtccctg atgacaacgg aagaggctac cgctccgaac
    24541 acagcgggca ggtacgaaat agtaccatag gccgacttct tatagttttt accatcggta
    24601 agcatcaggc tttcctttat cggttcttca ggggagaaaa ccacttttac tcccctgtat
    24661 attttatgct cctttagttt tttccggatc tgctgcgcaa agggacaatt ataggttttg
    24721 gaaatatcca ccacctgtaa ccgggtggga tctaatttgg cgccggctcc catactgctc
    24781 accaatggca atttactttc ataagccagg cggataaaag taaccttagg tgtgatgctg
    24841 tcaatagcat cgatgatata atcagggcga tgcgacaatt gctgcagtac catctccggg
    24901 ttaatgaatt ctttgatgat gtgaagttgc agttcggggt taatggcttt cagcctctct
    24961 gccataatca ttgctttgga tacaccgtga ttggtagcga gggcaggcaa ttgacggttg
    25021 cggttggtcg ggtctaccac atcaccatca atgatggtca tctgacctac accactgcgg
    25081 gcaatgaatt cggccgcata ggaacccacg ccacctaatc ccactaccat aacatgcgat
    25141 gtggccagtt gttgcaacgc cttttctccg atgagcaatt gtgtccgtga taaccaggat
    25201 agatccgttg tcatagctga acagcagcag ggccaaatac agccgctgca tttttttgaa
    25261 gctgcaaact aagtgaattg tggtcaattg ataaagtact ggctgcaaga tcatacaccg
    25321 tttctatgga aagtgcagcg tcatctgttt ccaggaaaaa gcggtcggaa ggaatggtac
    25381 ccaaacactc tcttactctt tcctgctgca aagcttttcc gaatgagagg taatatccct
    25441 cactgataat acggctggcc attactgcgt tttttgcaaa gccatgaaag atgacgggaa
    25501 ctgtatttcc ctgttttttc agtagtgaaa acacttcctc ccatgccctt acacaatgaa
    25561 tgaccagtgg tttttgtata ctgttagcca atcttatttg tccggtaaat actagctgct
    25621 gcagggaaaa atcagtgctg catactttat cgaggccaca ttccccaatg gccagtacat
    25681 gcggatgagt gctccattgc tccagttgcc ggagctgttc ctgcccggtt ccttctatat
    25741 accaggggtg aatgccaatg gaaaaccggc ccggtataac agctttttcc caatgatcat
    25801 gcaggttttg gatcatccat tcattgggag acggggcatt atgactatgc atattgatat
    25861 acaaaacggg tgattgtgcg gcacaattta agtactgcaa cggatactgc aggagacgat
    25921 atactgattt tcccaatgaa aacggaagaa catcacctct ttttcccaat gaaaaatgtg
    25981 catattcaga gggaaaaaaa gttttttctc agtgagttgg ataaataaat actctatatt
    26041 tgataccggt aatactgaaa acccgttaac aattcaaaat ctaaaacaac atggcaacta
    26101 agaaaaaacc cgccaagaaa gctgctaaga agcctgcagc aaaaaaacca gctgctaaga
    26161 agccagctaa aaaagctgct aagaaacccg cagcaaaaaa acctgctaag aaaaaatcag
    26221 cccgcaagcc taatgctgct ttcatgaaag cattaacgcc aagtgctgac ctggcagcag
    26281 tggttggtaa caaaccattg gcccgtacag aagcagttaa gaaaatctgg gcgcacatca
    26341 aagccaacaa actgcaggac agcaagaaca ggcgtatgat caacaacgac gccaagctga
    26401 aacccatctt cggtggtaaa gcacaggttt caatgtttga tgtagcaaaa gctcttgcta
    26461 agcatctgaa ataattggtt cctacggtga acaaaaaggg catctgtaca ggtgcccttt
    26521 tttattgcct gcagtgatat aatttctctt ctgtattttt aatattcaat cnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26641 nnnnnnnnnn nnnnncggaa aaattcagtc ctggatgaag gatgtcagta ttataccaag
    26701 atcagagtgg aaagccaatg atcccatacc ttacctgcgg catgagccgg ttcgcattac
    26761 ggtacatcat gaaggaacaa agctgctggt gacggatgat gcagtaagaa aaataaaagg
    26821 tattcagaca tggggcatgg gaaaggaccg caactgggcc gatgtgcctt atcatttctt
    26881 tattgcaccc gatggtaaga tctatgaagg aagaaatata aatacagtcg gggaaaccaa
    26941 tactg
//
LOCUS       DAORLS010000008        25166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1074_length_25166_cov_1.876030, whole genome shotgun sequence.
ACCESSION   DAORLS010000008 DAORLS010000000
VERSION     DAORLS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 25166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25166
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1074_length_25166_cov_1.876030"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    407..425
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <426..1523
                     /locus_tag="PK977_01390"
     CDS             <426..1523
                     /locus_tag="PK977_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRF16782.1"
                     /translation="FNVRLNQVKTKIESAFRAYLNNNDQEYPDKDHLMKILDDLFQKS
                     KKQNANRFLDYVTKFIEDAKTRLNPKTGKKISENTIKTYNTLLNHIKEFESKKQRTLK
                     FKHLDLEFYADYTDFLVDEYKLSTNAIGKDIHILKAILNDSIEKGINKHTNFKSPRFK
                     VLREESDSIYLTEKEIQELYLLDLSSSKTLEVVRDLFVIGCYTGQRYSDYTNIKPENI
                     TPNGDIRIKSVKTDQYITIPVLPIVKEILDKYDGKLPKAISNQKTNEYLKDIGKKVES
                     LKAIVGKTITKAGKRIDLTKAKWELLTTHTARRSFATNHYLKKYPTLAIMAITGHKTE
                     KAFLKYIKVGQSDQVQHFKKIAQSESTLKAV"
     gene            1533..2243
                     /locus_tag="PK977_01395"
     CDS             1533..2243
                     /locus_tag="PK977_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16783.1"
                     /translation="MEPILNNEESLLSQEEVDQISYIWVKRKRLIADISEKLHSSFKL
                     NRVEVEDEINDWLDFIRLKTYHIELASESKLPILTVLKNSCQPFLDVSKSDILKAHHY
                     LQMEFDYAVNDYPESIGHTEVIGYIVSTAVIKYLNSIPNSTLSRKRLSHRAKINLLSK
                     IGLTEILNQKNVKTASDIGIFLTILLDIEESNARKLAANILEPKSSSYDRYDINDPQV
                     EQEVKEVLKLRLPNSLIA"
     gene            2322..2642
                     /locus_tag="PK977_01400"
     CDS             2322..2642
                     /locus_tag="PK977_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF16784.1"
                     /translation="MTEKIIFTSLGIEELRSLIVDSVSSELKKQLATSTSTTKSDRLL
                     SRQETARLLNVSTSTLLSYVKQGRIKSFRIGRRVLFKQADIEAAMKLIITDPLLIQSN
                     DRQA"
     gene            2626..3546
                     /locus_tag="PK977_01405"
     CDS             2626..3546
                     /locus_tag="PK977_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16785.1"
                     /translation="MIDRHRGNCILNEGLDPVMNQVDHTFEAFGLTFFPKKVGLVYDS
                     EGLFLNTFKLRLVYPDELIVINSLHKFLHNGFNVYDVTYQEMVQGLHKLYSSLPVNLS
                     TVKISQIEFGCAIQVEQPLDLSQSLLYFENKPMLQYNENGNIIGKWHNAQQYKLKFYS
                     KYHEMAQCLSTLNRFKQSGGDIDYLRKNLRTEFVIKRSRLLNRATPLSLYDFENKTLI
                     KQIYLDFHKRCKKLTFQNGTLPSCCNESELFASALFENPNISYQRRLKTMSSIKYDYY
                     KRLYQSFPRAKYEKQRFLEAIEDKINTLFK"
     gene            complement(3699..>4115)
                     /locus_tag="PK977_01410"
     CDS             complement(3699..>4115)
                     /locus_tag="PK977_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16786.1"
                     /translation="RIDGGTVNLNEVISYSKGGQTVSVNSNLSSQPNFSNAAPKDCEL
                     KNVGDVLFENKSNSEITVYIYAIGVQPDGRSGQQTNNPIIATVSVPANGTASAYDLTV
                     GTHFIYFISTGYRYSGQIRIRKCETSREALRVQNLY"
     assembly_gap    4116..4263
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4264..5257)
                     /locus_tag="PK977_01415"
     CDS             complement(<4264..5257)
                     /locus_tag="PK977_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16787.1"
                     /translation="MHKYRFCSLSILLLLVLLISGCWARLLMTRSVALRTTIAEAGAS
                     RALTVSSRGVFIADQAVLNSTLDKVFIEGDALFTRYQGIPTRPFGRIVNRNTISIGSY
                     GLFEVPGEIYKIRGNGVRVRSDAGDIYSVLQLRNTGDVVLKLKEKDGWAYVQSSKEIF
                     GWISLAFLVAASENGPSGVTFVQCHVCNGQGHGIRDVNCTMCHSSGSIKCGYCGGNGG
                     GGCLECSGAGQIKCFHCNGEGSKKCERCFGVGFSKDHNGIDISCSYCGGNKNKICIDC
                     SGQGSRKCITCQETGFLHCNYCQGSGLDVCPQCFKSGKTVQDVFCVKCNGSGWLK"
     gene            5423..7882
                     /locus_tag="PK977_01420"
     CDS             5423..7882
                     /locus_tag="PK977_01420"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HRF16788.1"
                     /translation="MAIKKSELYSSLWEMCDELRGGMDASEYKDYVLIMLFIKYISDK
                     YAGVPYAPITVPKGASFQDMVALKGKDTIGDDINKKIIGPIEQANKSLGSIKVDFDNP
                     EKLGTGKEKVDRLTKLIAIFERPELNFSKNKAEGDDILGDAYEYLMRNFATESGKSKG
                     QFYTPAEVSRVMAKIIGINQKKTSSDTTIYDPTCGSGSLLLKVADEAEKKITIYGQEK
                     ESATASLARMNMVLHDCPTASIKCTGNSTLSDPQFKEESGGLKLFDFVVANPPFSSKN
                     WSNGLNVITADADEYGRFKGYGIPPAKNGDYAFLLHIIRSLKSKGKAAVILPHCVLFR
                     GNAEAEIRKNLIKKGYIKGIIGLPANLFYGTGIPACLIIIDKENAENRKALFMVDASK
                     GFTKDGPKNRLRDQDIHKIVDVFNKQLELPKYSRIVPIAEIEEKEFNLNIPRYIDSQE
                     EEDIQDIDAHLNGGIPDHDLEALSDYWEVFPTMKKELFAPLRKGYSRLKIEPAAIKSA
                     ILDHPEFSTFSQNAHQLYDGWKKKVVPVLNSMQEGVKPKQLIHEVSEALLSAFADLPL
                     LDKYDVYQHLMSYWSEVMQDDVFVISEDGWKAGREVYRMVKTSKDKGARAREIPGLEG
                     IESKLLKPELLVNRYFKKEKEAIEALETEREGISQQIEEMEEEHGGEEGLLNEARNDK
                     DKITVASAKERMKKIKKSTEDKEEYELLQNYVELNEEKARLGREISDKQVELEKLVWD
                     QYGLLTDEEIKSVVVYDKWLTVIDQAIQTELQRVSQRLTQRIKELGDRYDTTLPTLLN
                     ELNQLEQKVNAHLAKMGFVWS"
     gene            7873..9108
                     /locus_tag="PK977_01425"
     CDS             7873..9108
                     /locus_tag="PK977_01425"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRF16789.1"
                     /translation="MELKKGYKKSKLGYIPTDWIECSFEDVLIGFSSGQTPYRAIGRY
                     YTGNIPWITSGELNYNVITDTNEKITEEAVKKTNLKKLPIGTFLIAITGLEAEGTRGS
                     CAITGVEATTNQSCMALYPKQSMITTEYLFQFYTQYGNWLAFNYRQGTKQQSYTGKIA
                     KKLPIILPPTLKEQTAIATALSDMDELINSLEKLIEKKKAIKQGVMQELLRPKEGWVV
                     NAPLGNIIYLQGGYAFRSGLFKDIGVPIIRISDVGEGGVSLHNCVKYDEINLSESFCV
                     TKGDVLIAMSGATTGKIGVYQESIKAFINQRVGKFCVHDKRKSSLEFISHLVRGNKFR
                     LGLQKELAQGAQPNISGKQIEAIEVDYPESIDEQLKIATILSDMDDEIMLLEVNLVKY
                     KLLKQGMMQELLTGKTRLV"
     assembly_gap    9235..9414
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9415..9681
                     /locus_tag="PK977_01430"
     CDS             <9415..9681
                     /locus_tag="PK977_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16790.1"
                     /translation="DYFKIRDMPNNIIKTDHVNNRKLVEDIENVIIQSIRQDSLINDK
                     KKIGSSKKVTNEDAIAIADFIMNSNSIKKNNVSNTKVKFRKVAK"
     gene            9732..10496
                     /locus_tag="PK977_01435"
     CDS             9732..10496
                     /locus_tag="PK977_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16791.1"
                     /translation="MDKIAHINTVIQDYFDKHKNISRVPAKDLMPYFVKAGVFSKDTK
                     QGLPIRNILRQLQKSKQLHQIPFVMAEQKDVNTSWFFGRGPFKVNANLTPVAKKEVPT
                     KQPPTSSRTRKESDEHYIIDLCDQVLGLQGSRQHRFDFLLGDPGKNGRATRLPVDVYY
                     ESLRLVIEFNEKQHSQPVRFFDKPDKMTVSGVSRGEQRKIYDQRKRTILPKHGIKVIS
                     IDHQDFTCDRRGKLMRKRADDLAVITALLKGFIKKP"
     gene            10527..10757
                     /locus_tag="PK977_01440"
     CDS             10527..10757
                     /locus_tag="PK977_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16792.1"
                     /translation="MKEIEFITNEKMKDVLLLLEEAANRKADVAFNLFTISNYAAYLE
                     NFHSDILKSLLDPSSHHGEGYMPLQTFISYLP"
     gene            10708..>11093
                     /locus_tag="PK977_01445"
     CDS             10708..>11093
                     /locus_tag="PK977_01445"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRF16793.1"
                     /translation="MERDICLYKLLFPISHNAQIDINEFQETDVLREKGRIDVWIRDN
                     KSGKCILIENKINNAVDREDQLLDYYRYSKDMGFSPLAIVYLSLDGKKTAPEQSHPLD
                     ISIINVPAFNNTEKDLVNGWLNYCIE"
     assembly_gap    11094..11107
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11163..>11366
                     /locus_tag="PK977_01450"
     CDS             11163..>11366
                     /locus_tag="PK977_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16794.1"
                     /translation="MDNKSREEFYNYASTLPTLETLQNIQYLINDLPAYRAQKFNDQF
                     EGIVPFKKKFLYTSCHWVFEGFIY"
     assembly_gap    11367..11397
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11398..11659
                     /locus_tag="PK977_01455"
     CDS             <11398..11659
                     /locus_tag="PK977_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16795.1"
                     /translation="EDSGQANVVIWNTIKKENGGYAATKELLEKVNLIDKFNGQSRFN
                     GFSAEFKIPGIFKSIEEIDKAVVTFVKEVLTKLNAVQDGTSR"
     gene            11643..>12525
                     /locus_tag="PK977_01460"
     CDS             11643..>12525
                     /locus_tag="PK977_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HRF16796.1"
                     /translation="MEQVGEKERYTQNRIVKLFREEMGYQYLGNWEDNPNNSNIEETY
                     LRQYLEEKAGYPKDLINRAIYKLKSAANNANGHLYNSNKEVYKLLRYGVEVSANPGDR
                     YQRIQFINWDEPEKNHFGIAEEVTFRGYREKRPDIVLYINGIAVAVLELKRSIVSIGE
                     GIRQNLVNQQKEFIEDFFTTIQLVMAGSDSEGLRYGTIGTPDKFFLRWKEDEEDNSRL
                     QLDKYLIKICQKSRLLEIIRDFVLFDAGVKKLPRYHQYFGIQAAQEHIRRREGGIIWH
                     TQGSGKSIVMVLLAQWIL"
     assembly_gap    12526..12738
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12739..>12955
                     /locus_tag="PK977_01465"
     CDS             <12739..>12955
                     /locus_tag="PK977_01465"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRF16797.1"
                     /translation="DAFIEELKNGPSKTVGEIFVFVDECHRTQSGKLHKAMKALLQNA
                     VFIGFTGTPLLKSDKQTSLEVFGKYIHT"
     assembly_gap    12956..13041
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13042..>13585
                     /locus_tag="PK977_01470"
     CDS             <13042..>13585
                     /locus_tag="PK977_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16798.1"
                     /translation="KLSSPEKVDAWFEAKTKGLNDFQKSELKKKWGTMQKVLSSRSRM
                     DKVVSDIVFDFSKKTRLNSHTGNAILVAGSIYEACKYYELFKNTEFKNRCAIITSYNP
                     QTKDITTEDTGANTETEREFIYKTYTELLNNVQPSPGKSKTETYEDLAKDKFIKEPAN
                     MRLLIVVDKLLTGFDAPSCTY"
     assembly_gap    13586..13677
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13678..>14134
                     /locus_tag="PK977_01475"
     CDS             <13678..>14134
                     /locus_tag="PK977_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16799.1"
                     /translation="KQFGYIVDYKDLFKKVENAVAVYTSELDYDEFKKEDCDILLKDR
                     LKKGRERLDLALEEIELICEEVSPPKSKLDYQHYFCGNPEIQDELKNTEMRRTALYKA
                     TVALIRAYANITAEMDEAGYTETEQEDIKMKVDFYIKLREEIRLASGETL"
     assembly_gap    14135..14146
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14147..14746
                     /locus_tag="PK977_01480"
     CDS             <14147..14746
                     /locus_tag="PK977_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16800.1"
                     /translation="KTYEADMRHLIDNYIQAEDSRAISPFQDMSLIDIIVKSGIAEAI
                     SKMPGGIKGSKQAVSETIENNVRQKIIKDHLLDPAFFEEMSKLLDEIIKERRANAIDY
                     EEYLKKIADIAAKVQSGKSEKTPDDIKTPAQRALYNNLGKSEALALKLHDAILRVKPD
                     GWRGNEPRENAIKAALYEILQDFGEVERIFDIVKQQREY"
     gene            14746..>15116
                     /locus_tag="PK977_01485"
     CDS             14746..>15116
                     /locus_tag="PK977_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRF16801.1"
                     /translation="MEKISLGDIVVDVELKDIKNVHLSVYPPKGRVRIAAPRRMSLDT
                     IRLYAISKLGWIRQQQKKFREQTREAPRDFLNRETHYFMGVRYMLKVVEHDAAPSVRI
                     KHKSLELSVRPGTGKAKRQEI"
     assembly_gap    15117..15146
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15147..15468
                     /locus_tag="PK977_01490"
     CDS             <15147..15468
                     /locus_tag="PK977_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF16802.1"
                     /translation="LKELVPSIISKWEKKMKVSVSEFGIKRMKTKWGTCNREAKRIWL
                     NLELAKKPLSCIEYIVVHEMVHLLERNHNDRFISFMDRFLPQWKELKKELNRLPVSHR
                     DWEY"
     gene            15513..16373
                     /locus_tag="PK977_01495"
     CDS             15513..16373
                     /locus_tag="PK977_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16803.1"
                     /translation="MKATFEEKSFENYFNSELDKKASIFFPFGQVQEGFLGFDASSFS
                     KNRSLWRAIGYPFLFSPKFKGADLIEIAEEMENYLSVTINNMPNLKANLLFQYKRPEY
                     VTSPKCAEWYLWNKPYYRYNIDMNQQLLLSKLHSSFSGRLLILYVSPAFHEINDLVHY
                     KQKNKIIKNSNFTKAIDLNGHSKNTYITKGTYSIACSEPKRIKKINLENQLDALRSNI
                     SKFENNTQFIKDFSSQIRDIVSEDATYSNAFLRLNSEYSMLTDYSVFHSLLVMANFKN
                     LTGAQWIITT"
     gene            16773..17699
                     /locus_tag="PK977_01500"
     CDS             16773..17699
                     /locus_tag="PK977_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16804.1"
                     /translation="MIDKLDIQRKFQNETFYKRLEIDLQKGCIPPAITIAFIKETDFN
                     NPITLNEAERQINDDISTAFILDGIQRLNTIKRIVSQDNNELDLERYLYVNIIICPSR
                     DNLLYRMVTLNNGQKPMTARHQIEILAESIYDFSSYHFQVFTEKENADKKEKGFKKAD
                     IINAYISFLSNSTSLDNKKIIEDKLDELVARKIIESKITDDSVDFNKVLKLVGRMYES
                     ESLYNWLKNSNNLIGFSVGIRNSYIHVNALNNEELEIQLSKFEKAFKNINVSTIKLSR
                     ERRRLTSFFINKLHDLIEKDSDELTLLLAEQI"
     gene            17707..18474
                     /locus_tag="PK977_01505"
     CDS             17707..18474
                     /locus_tag="PK977_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16805.1"
                     /translation="MSLYLFSFKDPIPPNYRSAVPPYFRVTTATTYDVLLIRLLTSTL
                     DISNSKTNKNIKLTSNFTVFPAFTVSRKSAKQIRAFISPFNLSDYIKYSQKYYGRNIT
                     FYHNLLQELTFYFYYSSENQFQSAFVNLYRTLEYLSYSFPLMHASHFGNYIGSFEALR
                     AYFTDANTSELKFFESFVARLFQGTPYLSLTTNFDFSHPDILVANNCFDSFKGLMKAT
                     DWTTANKLTHSLSIENSNLIKLFKNVRNRYFHFAVGG"
     gene            complement(18705..19463)
                     /locus_tag="PK977_01510"
     CDS             complement(18705..19463)
                     /locus_tag="PK977_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16806.1"
                     /translation="MKTIVKFGSVVLASFLCLFLLGNSAYSQNSKKIKNGTSNSIQPF
                     PETDINLEDKIRERQERYDKEEERRIQLNIREREESYTPLILNRVYKYLLPVALLLII
                     IFSSVSQLNIRQRIIIPFFLLIGLYLLGSLSVPQGGSATEYFENTILPGAISLVLAIL
                     LIGGSVYFLCEMPLPTKNHKIFIVLLTAVITGLLTLILTKSIILSIEDSNDIEAGKTL
                     FILYPFNLLGFFIYNAFVYYRVFLSKASGDIIVK"
     assembly_gap    19726..19776
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19777..20217
                     /locus_tag="PK977_01515"
     CDS             <19777..20217
                     /locus_tag="PK977_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16807.1"
                     /translation="PLPEITPAPSRMCFYPLVEANYSFTLNLLKMVKVKQYHVRQGND
                     GEKYISLELEGDVSFVQSQNSGRFYATTKRCFMYAAMDEPTAKALVGTQMPGTIERVP
                     CDPYEYTVPETGEVKVLSYTYEYRPAEAVELPDMIGSARATTLA"
     gene            20293..21222
                     /locus_tag="PK977_01520"
     CDS             20293..21222
                     /locus_tag="PK977_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF16808.1"
                     /translation="MQLQKASRTKAKMKLALQGPSGSGKSIGALLIAFGLCGNWSKIA
                     VIDTENSSANLYADLGNYNTVSIGPPFSPEKYIQAIKLCEDSQMEVIIIDSVSHEWDG
                     AGGILDIHSNMSGNSFTNWGKLTHRHNAFVQAMLQSPCHIISTIRSKQDYVLNEKNGK
                     MVPEKVGLKGVQRDGLDYEFTIVLDIDSKHNAIASKDRTSLFISKPEFQITVETGKQI
                     NDWCNKGEDVTAIVTNSITKDVISEEQLLQEIKNCNTVDSLLLLYNKYPLYQETHVNQ
                     FTVRRKELLASQNSNPISHPLKPSANGTHTSAT"
     gene            21197..22198
                     /locus_tag="PK977_01525"
     CDS             21197..22198
                     /locus_tag="PK977_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3871 family protein"
                     /protein_id="HRF16809.1"
                     /translation="MELIQAPLNHQSVVSPAPETYLPAPVLSHGSAFIEANTVESSLE
                     EIKEKHIIPVFIKDNEPLISHADFISAAEEVVSDVFNGETILRPNIRLSHPIKGRIPE
                     AKDKPAIQLQEHEKTLYYERMAFVIEVPSIHDEIEGSQLSLTIGGVKSLNQDNLYNKK
                     GADEHFKVFIGFQNKVCTNLCVWTDGYMSDLKVTTLGQLQGCIRTLVDNYNAVYHLNR
                     LKDLTQFQLTEQQFALLIGRCRMYPHLPKQMQSEIQPLLFGDSQINTIVKDYYRDRSF
                     CRNEDGTINLWKLYNLFTGANKSTYIDNFLDRSVNAYSFIEQLKYALKNHDDNWFLN"
     gene            22531..22980
                     /locus_tag="PK977_01530"
     CDS             22531..22980
                     /locus_tag="PK977_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRF16810.1"
                     /translation="MIDIDQVSEISIRYKPKIAKRPVVKSSNDCYQVLKCWFDENTIH
                     LQEQFVVMYLNQGNRVLGMLKLSTGGITGTVADIRLILAVALKAAACSIIIAHNHPSG
                     NLQPSKADLDITRKLKAASELMDIRLLDHLILNPDNEYLSLADEGHI"
     gene            complement(23418..>23925)
                     /locus_tag="PK977_01535"
     CDS             complement(23418..>23925)
                     /locus_tag="PK977_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16811.1"
                     /translation="KIGDIFSVEIAKSKKRYFQYISIDKLQLNSDVIRIFRGNFESHE
                     SLDIDKLKDSGVESYIHCFVNQGIKMNIWKKEGYNSDLGLDLEIVFRSTDDYGGKVGS
                     QPLLYTNNWHIWQLGKSEEKAKVVSSNLNKLKCSHIGIVINPLGILEILKGNKYPPQY
                     PEIGNILF"
     assembly_gap    23926..23948
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23959..>25166)
                     /locus_tag="PK977_01540"
     CDS             complement(23959..>25166)
                     /locus_tag="PK977_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF16812.1"
                     /translation="ESPQDVYFDDLTVRHITGPLVEEKSYFPFGLVMGAISSRAVLKA
                     KTVYAYNAGSEWEEQGIEYYDTYLRKYDAQIGRFASIDILADYYLNFTPYNYSANNPI
                     YFNDPSGASLSFNNVDDVISYLWNETDHGGSWSALQNTIITFKDSFIGAYFTELQKIM
                     NLFNYVDDGNGGGTLYYNGYEVKEIYGGYRDKGNHSILGIGIIFSQSYNNGGGNLNGE
                     VLMGSNFIGISRHNEKSLIDYVQTGFDIAGLVPGIGELFDGLNAGIYALRGDYENAAL
                     SGAACIPFAGWGATGTKIARKTAKELKSGSYLVYKGVDANANVRYIGITSRDAVARFN
                     EHLNSSSAKALLDYEVISGAVGLSKEQARIIEQTLINQYGLMKNGGQLLNKINSIAPK
                     YWAIFGISK"
BASE COUNT         8047 a         4850 c         4739 g         6631 t
ORIGIN      
        1 atccttgaca caaggatttt cccaaataaa aaagagagcc attgctgact ctctttatgt
       61 ggtgtggggc ggacttgaac accttaagcc ttacaatttc tcttcccttc ttatttagtt
      121 gaatatcata tacttaaaga actatcaagt atcaataagg gtcaataagc agcaacgaaa
      181 atcaaaaaaa ccgctccgga aaccgctccg ggttttacct ttataataca tctagtgaga
      241 aatgataaat gtgaacttca atctacggga tcctaaagct gcaacttctg gcctgcgcat
      301 tattcttagg taccacaacc aacgctttta ctacccaatt aaagaaagga ttcctgttag
      361 acattgggat ggaaataatc agaaggcaaa aagatccttt gagggtnnnn nnnnnnnnnn
      421 nnnnntttaa cgttcgctta aaccaggtta aaacaaaaat cgaatccgcg tttagagcat
      481 atttaaacaa taacgaccag gaatatcctg ataaagatca tctcatgaag atcttggatg
      541 atttattcca aaagtccaaa aaacagaatg cgaatagatt cctggattat gttacaaagt
      601 tcattgagga tgcgaaaaca agactcaatc ctaaaacggg caaaaagata tctgaaaata
      661 caataaagac ctataacacc ttattgaatc atattaaaga gtttgaatct aaaaagcaac
      721 gaactctcaa atttaaacac cttgatcttg aattctatgc cgattatacg gactttttag
      781 ttgatgaata caaactatca acgaatgcaa ttgggaaaga catacacata cttaaagcca
      841 ttttaaatga ttctattgaa aagggtatta acaaacatac caatttcaaa agtccaagat
      901 tcaaagttct aagagaggaa tccgacagca tttacttgac agagaaagag attcaggaat
      961 tatacttact tgatctttct tcctcaaaaa cactagaagt agtcagagat ctgtttgtaa
     1021 ttggttgcta cacgggtcag cgatattcag attatacaaa tattaaacct gagaatataa
     1081 ccccgaatgg cgatatccgg attaaatcag taaaaacaga tcagtacata acgattccgg
     1141 tacttccaat tgttaaagaa atacttgata aatatgacgg aaagctaccg aaggccatta
     1201 gtaatcaaaa gaccaacgaa tacctaaaag atattgggaa aaaggtagaa tcactgaaag
     1261 caatagttgg taagacaatt acaaaagccg gcaaaagaat agatttaact aaagctaaat
     1321 gggaactact tactacacac acagctcgaa ggtcatttgc cacaaatcac tatctcaaga
     1381 agtatcctac gctggcaatc atggccataa ctggacataa aacagaaaag gcctttttaa
     1441 aatatatcaa agtaggtcag tctgaccagg tacaacattt caagaaaatt gctcaaagtg
     1501 aaagcactct aaaggcagtt taactaaata ctatggaacc aattctaaat aatgaggaat
     1561 cattgttatc tcaagaagaa gttgatcaaa tatcttatat atgggttaaa agaaagcgcc
     1621 tgattgcaga catttctgaa aaattacact cttctttcaa attaaatcgt gttgaagtcg
     1681 aagatgaaat taacgattgg ttggatttta taagactaaa aacctatcac attgaacttg
     1741 ccagtgaaag caaattgccc atacttactg ttcttaaaaa ctcctgccaa ccctttctgg
     1801 atgtttcaaa atcagatatc ttaaaggcac atcattatct tcagatggaa tttgactatg
     1861 ccgttaatga ttacccagaa tcaattggtc acacggaagt cattggctat atagtttcaa
     1921 ctgcagtcat taagtatcta aattcaattc ctaattcaac attatcaaga aaaagattaa
     1981 gccaccgagc aaaaatcaat ctgttatcta aaattggact taccgaaata cttaatcaga
     2041 aaaatgtaaa aactgcatca gatatcggaa tcttcttaac tattttatta gatattgaag
     2101 aatcaaatgc tagaaaatta gctgcaaata ttctagaacc aaagtcatca agctatgacc
     2161 ggtatgatat taatgacccc caggtcgaac aagaagttaa ggaagtcctg aaactaagat
     2221 taccgaattc attgatagcg tgaaatgagc cccttgatta tgccccaaat aatgccccag
     2281 cgatatagat aataacattt gtcatcttaa atattcctaa gatgacagaa aagatcattt
     2341 ttacctcact aggtatagaa gaactgagat cgctgattgt tgattcagta tcctccgagc
     2401 tcaaaaagca gctggcaaca agcacctcca ctacaaaatc cgatcgctta ctgagccgac
     2461 aagaaacggc caggcttctc aatgtttcaa cttcgaccct cctgtcctat gtcaaacaag
     2521 ggagaataaa atccttccgt attggacgtc gggtcctttt taagcaagca gatatagagg
     2581 cggctatgaa gctaatcatt actgacccgt tattaattca gagtaatgat agacaggcat
     2641 agagggaatt gtattttaaa cgagggtctt gacccggtta tgaaccaggt cgatcataca
     2701 tttgaagctt ttggattgac attctttcca aagaaagttg gcttggtata tgactcagag
     2761 ggcctattcc ttaatacatt taaacttcgg ctagtatacc cagacgaact gattgtaatc
     2821 aattcgctac ataaattcct gcataatggt tttaacgtat acgacgttac ttatcaggaa
     2881 atggtccagg gccttcataa gttatactcc tcacttcctg taaacttgtc aactgtcaaa
     2941 atatcgcaaa tcgaatttgg atgtgccatt caagttgaac aaccgcttga tctttctcaa
     3001 tcactccttt atttcgaaaa caaaccaatg cttcagtata atgaaaatgg caacattata
     3061 ggcaaatggc ataatgctca acagtataaa ttgaaattct actcaaaata tcatgaaatg
     3121 gctcagtgct tatcaacatt gaataggttc aagcaatctg gaggagatat tgattatctg
     3181 cgaaagaact taagaacaga atttgtcatt aaaagatcaa ggcttcttaa tcgcgcgact
     3241 cctctgagtc tatatgattt tgaaaataag acattaatca aacaaattta ccttgacttt
     3301 cataaacgat gtaagaagct gacatttcag aatgggactc tgccatcctg ctgtaatgag
     3361 tctgagctat ttgcatcagc actatttgag aatccaaata ttagctacca acgaagactt
     3421 aaaacaatgt cctcaattaa gtatgactat tataaacgct tataccaatc ctttccaaga
     3481 gccaaatatg aaaaacaacg ttttctagag gcaatagagg acaagataaa cacattattt
     3541 aaataactaa tgggacacga gatttaacgc tattaacatg atgttaaagc tgtcgactga
     3601 aacagactct ccagtagaaa tcataccagc acattttacg tagcgcgcaa cttccctgat
     3661 ctaagaattt actgccttga tatctacaaa taaagtgatt agtaaagatt ctgtaccctt
     3721 aaagcctctc tacttgtctc acactttctt atccttatct gccccgaata ccgataacca
     3781 gtcgaaataa aataaatgaa gtgagttccg actgttaaat catacgcact tgcggttcca
     3841 ttcgcaggca cagaaactgt tgcaataatt gggttattgg tttgctgacc actccggcca
     3901 tctggttgaa cgcctatagc ataaatatat acggttattt cagaatttga tttgttttca
     3961 aaaagaacat ctcctacgtt ctttaactca caatctttcg gcgctgcatt actgaaatta
     4021 ggctgactgc tcaaatttga gtttactgaa acagtttgcc ctcctttgct atacgaaata
     4081 acttcattta aattcacagt accaccatca attctnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnntctttaa ccacccagat ccattgcact ttacgcaaaa aacatcctga actgtcttac
     4321 cagatttgaa acattgagga cagacatcca atcctgatcc ctggcaataa ttacagtgca
     4381 aaaatccggt ttcttgacaa gtaatacatt ttctgcttcc ctggccagaa cagtcaatac
     4441 atatcttgtt tttgtttcca ccacaatatg aacagctaat gtcaatacca ttatgatctt
     4501 ttgaaaatcc aacgccaaaa catctttcac acttcttgct accctcccca ttacagtgaa
     4561 agcattttat ctgaccagcg ccagaacact ctaaacagcc tcctcctcca tttcctccgc
     4621 aatatccgca tttaatactt ccactactat gacacattgt acagtttaca tcacgtattc
     4681 catgaccttg accattacat acatgacact gaacaaaagt tacaccactc ggtccgtttt
     4741 cagatgcagc aactaaaaag gctagactaa tccagccaaa tatctccttt gaactttgaa
     4801 catatgccca gccgtccttt tcctttagct tcaatacaac gtcaccggta ttacgcagtt
     4861 gcaaaacact ataaatatct ccagcatctg accttactct tacgccattc cctcttattt
     4921 tatatatctc gcctggcact tcaaacagtc cgtaactacc aatagaaatg gtatttctgt
     4981 taacgatacg accaaatggc cttgttggaa taccctgata ccttgtaaaa agcgcatctc
     5041 cttcaataaa tactttatcg agcgtagaat ttaaaactgc ttgatctgca ataaaaacac
     5101 cccttgagct aactgttagt gcccgggatg cgccagcttc agctattgtt gtccgtaatg
     5161 ccacgctcct cgtcataaga agccttgccc agcatccgct tatcaacagg accaatagaa
     5221 gcaatattga taaactgcag aatctgtatt tatgcatagc agcctcgttt tatcttgcct
     5281 aaaatactct aaatattttg ataatctata attgttagac cgcttgccaa atgttttagt
     5341 aagctgggta caaaacttga atcatactca gtaaacatcc aataaaaacg gtattattga
     5401 aaaacagatt acaagtttat ctatggcaat caagaaatca gaactctact cctccctttg
     5461 ggaaatgtgc gatgaactcc ggggcggcat ggacgccagt gagtacaagg attacgtcct
     5521 catcatgctt tttatcaagt acatcagcga taaatacgcc ggggttccct acgcgcccat
     5581 caccgtcccg aagggcgcga gcttccagga tatggtggcc ctgaagggca aggataccat
     5641 tggcgatgat atcaataaga aaatcatcgg cccgattgag caggccaata aatccctggg
     5701 atccatcaag gtggacttcg ataaccccga aaagctcggc accgggaagg aaaaggtgga
     5761 caggctcacg aaactcattg ccattttcga gaggcccgaa ttgaatttta gtaagaacaa
     5821 ggccgaaggt gacgatatcc tcggtgatgc ttatgaatac ctgatgcgga atttcgctac
     5881 agagagcggc aagagcaagg gccagttcta taccccggcg gaagtcagcc gcgtgatggc
     5941 caagatcatc gggatcaacc agaagaagac cagttccgat acgaccatct atgaccccac
     6001 ctgcggatca ggatcgttgc tcctgaaagt ggccgatgaa gcggaaaaga agataaccat
     6061 ctacggacag gagaaagaga gcgctacggc ctccctggcc cgtatgaaca tggttttgca
     6121 cgactgcccc accgcatcga ttaagtgcac cgggaacagc accctgagcg atccgcaatt
     6181 caaggaagaa tccggagggc tcaaactgtt cgacttcgtg gtagccaatc cccccttttc
     6241 ttccaaaaac tggagtaatg gcctcaatgt aatcacagct gacgcggatg agtatggccg
     6301 tttcaagggg tacggcatac ccccggccaa gaatggtgac tatgcttttc tgctgcatat
     6361 catccgctcg ctcaaaagca agggtaaggc ggcggtcatc ctgccgcatt gtgttttgtt
     6421 ccgcggcaat gccgaggcgg aaatacggaa gaacctcatc aagaagggat acatcaaggg
     6481 tattatcggc ctgccggcca acttgtttta cggcaccggc ataccggcct gcctgatcat
     6541 catcgataag gagaatgccg aaaacaggaa agccctcttc atggtggatg ccagtaaggg
     6601 gtttacgaaa gatggcccca aaaaccggct gcgtgatcag gacattcaca agatcgtgga
     6661 tgtatttaat aagcaactgg agctgcctaa atacagccgc attgtaccga tagcggagat
     6721 tgaagagaag gaattcaacc tcaatattcc ccggtatatc gacagccagg aagaggaaga
     6781 tatacaggat attgatgcac atctcaacgg tggcataccc gaccatgacc tggaggccct
     6841 ctctgattat tgggaggtat ttccaaccat gaaaaaggag ttgtttgcac cattacgaaa
     6901 agggtacagc cgcttgaaaa tagagccggc agccatcaag tctgcaatac tcgatcatcc
     6961 ggagttcagc actttcagcc aaaacgcaca tcaattgtac gatggttgga agaaaaaggt
     7021 agtgcctgta ttgaacagca tgcaggaggg ggtgaagccc aaacaactga tacatgaagt
     7081 atctgaagcc ttattgtctg cttttgccga tctgcccctg ctggacaaat acgatgtata
     7141 tcagcatctg atgagttact ggagtgaagt gatgcaggat gatgtatttg tgatttcaga
     7201 ggatggctgg aaagctggcc gtgaagtgta ccggatggtc aagacatcca aagacaaggg
     7261 cgccagggcc agggaaatac ccggcctcga aggaattgaa agcaaactgc taaagccgga
     7321 actcctggtg aaccggtatt ttaaaaagga aaaggaagca attgaggctc tggaaaccga
     7381 acgagaaggg atcagccagc aaatagaaga aatggaggaa gaacacggag gggaagaggg
     7441 gctactgaat gaggcgagaa atgataagga taagatcacg gttgcttctg caaaagagcg
     7501 catgaagaaa ataaagaaaa gcaccgagga taaagaagaa tacgaactgc tgcaaaatta
     7561 tgtggaacta aatgaggaga aagcaagatt gggaagggaa atatcagaca agcaggtaga
     7621 actagaaaaa ctggtatggg accagtacgg attgttaacg gatgaggaaa ttaaatctgt
     7681 ggtagtatat gataaatggt tgacggtgat agaccaagcc atccaaactg agttgcagcg
     7741 tgtcagccaa cggctcacgc aacggattaa ggaactggga gaccgctatg atactacatt
     7801 gcccacattg ctgaacgaat taaaccaatt ggaacaaaaa gtgaatgcgc accttgccaa
     7861 aatgggattt gtatggagtt aaaaaaagga tataagaaat caaaactggg atatattcca
     7921 acagattgga ttgagtgttc atttgaagac gtactaattg ggttttcaag tggccaaact
     7981 ccttacagag ctattggaag gtattatact gggaatattc catggattac aagcggagaa
     8041 ttaaactata atgtaatcac tgatacaaat gagaaaatta ctgaagaggc tgtaaaaaag
     8101 acaaatttga aaaaactacc tattggaaca tttcttatcg ctattacagg attagaggca
     8161 gaaggcacaa ggggtagttg cgctataact ggagtagagg caacaacaaa ccaatcatgt
     8221 atggctctat atcccaaaca aagcatgatt acaacagaat atctatttca attttatacc
     8281 caatatggca attggcttgc gttcaattat cgtcaaggaa ctaaacagca aagctataca
     8341 ggcaagattg ccaagaaatt gcctattata ttacctccaa ctttaaaaga acaaacggcc
     8401 attgccacgg ccttgagtga tatggatgag ttgatcaata gcctggaaaa gctgattgaa
     8461 aagaagaaag ccatcaagca aggtgtgatg caggaattgt tgaggccgaa ggagggatgg
     8521 gtggtgaatg ctcctttagg aaatattatt tatttacaag gaggttatgc atttagaagt
     8581 ggattgttta aagatattgg agttccgata attcgaattt cagatgtagg tgaaggaggt
     8641 gtttctcttc acaattgtgt taagtatgat gaaataaact tgtcagaatc tttttgcgta
     8701 acaaaaggtg atgtattaat tgctatgtca ggcgctacaa caggaaaaat aggcgtatat
     8761 caggagtcta ttaaagcgtt tataaatcaa cgtgtcggca aattctgtgt tcacgacaaa
     8821 aggaaatcat cactagaatt tatttcacat ttagtgcgtg gaaataaatt tcgtcttgga
     8881 ctccaaaagg aattagctca aggagctcaa ccaaatataa gtggtaagca gattgaggct
     8941 attgaagtcg attatcctga gagtatagat gaacaattaa aaattgcaac tattctctct
     9001 gacatggatg atgaaatcat gttgctggag gtaaatcttg taaaatacaa actgctcaag
     9061 caaggcatga tgcaggaact gctaacaggc aaaaccaggc tggtatgagc aagatgaaat
     9121 cacatatatc aatagctttt aactccgatt ggtttcagtc agggtatttc gtttatataa
     9181 ttaccgtaaa gcataagggg aaaaagatct actacattgg tcaaacaggg gaccnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngattac
     9421 ttcaagatca gagatatgcc aaataacata attaaaactg atcacgtcaa taacaggaaa
     9481 cttgttgagg atattgaaaa cgtaatcatt caatctatta ggcaagattc tttaatcaat
     9541 gataaaaaga aaataggaag ttcaaagaag gtcacaaatg aggatgcaat tgctattgcg
     9601 gattttataa tgaatagcaa ttctataaaa aagaataatg tttctaatac aaaagtcaaa
     9661 tttagaaaag tggcgaaata atggcagtta gaaattaacc cccacataac cggataagta
     9721 aatcgaattg tatggacaag atagcacata tcaacacggt catacaagac tactttgata
     9781 aacacaagaa tatcagcagg gtaccggcaa aagacctaat gccctatttt gtaaaggccg
     9841 gtgtattttc aaaagatacc aagcagggtt tacctatccg taacatattg cgccagcttc
     9901 agaaaagcaa acaacttcac cagatacctt ttgttatggc cgaacagaag gacgtcaata
     9961 cgtcctggtt ctttggccgt ggtccgttta aagtaaatgc caatcttacg cctgttgcaa
    10021 aaaaggaagt gcctacaaag cagcccccta catcttcacg aacacgtaaa gaaagcgatg
    10081 agcactatat tatagacctt tgcgatcagg tattaggatt acaaggttcc cgccagcacc
    10141 gctttgattt tctgctcgga gacccgggaa agaacggacg tgctacacgc cttccggtag
    10201 atgtatatta cgagagtctt cgactggtga tagagttcaa tgaaaagcag cacagtcagc
    10261 cggtcaggtt tttcgacaaa cctgataaaa tgacggttag tggcgtatca cggggtgagc
    10321 agaggaaaat ctatgatcag cgaaaaagaa ccatcctgcc caaacacggg ataaaagtca
    10381 tcagtatcga tcatcaggat tttacttgtg acaggcgggg taaactaatg aggaaaagag
    10441 cagatgatct tgcagttatt accgcattgt tgaaaggctt tataaagaag ccataacagg
    10501 aattaagaaa tattaataat ttaattgtga aagaaattga gttcattaca aacgaaaaga
    10561 tgaaggatgt gttgttgctt ttggaagaag cggcaaacag gaaagcagac gtcgcattca
    10621 acttgttcac tatttcgaac tatgctgctt atctcgagaa cttccatagt gatattttaa
    10681 aatcactgct tgatccatct tctcatcatg gagagggata tatgccttta caaactttta
    10741 tttcctatct cccataatgc acaaattgac attaatgagt ttcaagaaac agatgtatta
    10801 agagaaaagg gccgtattga tgtttggatt agagataata agagtggaaa atgcatatta
    10861 atagagaaca aaataaataa tgcggttgac cgggaagatc aactgttaga ttattaccga
    10921 tattcaaaag acatggggtt ttctcctttg gcgattgttt acctgtcgct tgatggtaag
    10981 aaaacagctc cggaacaatc tcatccgtta gatatttcca tcattaatgt gccagcattt
    11041 aataatactg agaaggatct cgtaaatggc tggttaaact attgtatcga attnnnnnnn
    11101 nnnnnnntaa atcattcgta caacaataca ataatctaat aagccattta tcaaatcaac
    11161 atatggataa taagtcaaga gaagagttct ataactacgc tagtacttta cctacattag
    11221 agacattaca aaatattcaa tacctcatta atgacttacc tgcttacagg gctcaaaagt
    11281 ttaatgatca atttgaggga atagtaccat tcaaaaagaa gtttctgtat acgtcgtgtc
    11341 attgggtttt tgaaggattt atttacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga
    11401 agatagcggt caagccaatg tggtaatctg gaatacaatc aagaaagaaa atggtggtta
    11461 tgcggctaca aaagaattgt tggagaaagt taatttgatt gataaattta atggtcaatc
    11521 aagatttaat ggattttcgg ccgaattcaa gataccaggg atatttaaaa gcatagagga
    11581 gattgataag gcggtggtta catttgtcaa agaggtcctt accaaattaa atgctgtaca
    11641 ggatggaaca agtaggtgaa aaagaacgat atacccaaaa ccggatagtc aaactcttcc
    11701 gggaagagat gggctatcaa tacctgggca attgggagga taatcccaat aacagcaaca
    11761 ttgaggaaac atatcttcgt caatacctgg aagaaaaggc aggttatccc aaagacctga
    11821 tcaatcgggc tatctataaa ttaaaatcag ccgccaataa cgccaacggt catttataca
    11881 acagcaataa ggaggtatat aaactacttc gctatggtgt ggaggtttct gccaatcccg
    11941 gcgatcgtta ccaaagaatc cagttcatca attgggatga gccggaaaag aaccattttg
    12001 ggattgcaga ggaagttacc tttcgtggtt accgggaaaa gcgcccggat attgtactat
    12061 acataaatgg tattgctgtt gccgtacttg aactaaaacg aagcatcgta tccattggag
    12121 agggtattcg ccagaacctg gtcaatcagc aaaaagagtt cattgaagat ttctttacta
    12181 caatacaact cgtcatggcc ggtagcgatt cagagggtct tcggtatggc acgatcggca
    12241 ccccggataa gttctttctg agatggaagg aggacgagga ggacaacagc cggttacaat
    12301 tggataaata cctgatcaaa atctgtcaga aatcccggtt actggaaatc atacgtgact
    12361 ttgtcctgtt tgatgctggt gtaaagaaac tgccgcggta tcaccagtat tttggtatac
    12421 aggccgccca ggaacatata cggcgtaggg aaggtggtat catctggcat actcagggca
    12481 gtggtaaaag tatcgtcatg gtgctcctgg cacaatggat tctaannnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnntg atgcttttat tgaggaactg aaaaacggcc catccaaaac
    12781 ggtaggtgag atattcgtat ttgtagatga gtgccatcgt acccagagtg gcaaactgca
    12841 taaggccatg aaagccctgc tgcagaatgc ggtatttatc ggctttaccg gtacaccact
    12901 gttaaaatca gataagcaaa ccagtctgga agtctttggt aaatacatac acaccnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn naaagctatc ttctcccgaa aaggttgatg cctggtttga
    13081 agcaaagaca aaaggattaa atgactttca gaaatcagaa ttaaagaaaa aatggggtac
    13141 gatgcaaaag gtgctcagtt cccgctcccg gatggataag gtggtcagtg atattgtttt
    13201 tgatttcagt aaaaaaaccc ggttgaattc ccatacaggc aatgccatcc tggtggcagg
    13261 cagtatctat gaggcctgta aatactatga actgttcaaa aatacagaat tcaaaaaccg
    13321 atgtgccata atcacatcct ataaccccca gacaaaagac attactactg aggacacagg
    13381 tgccaatact gaaacagaaa gggagtttat ctataaaacg tacactgaat tattgaataa
    13441 tgtacaaccc tcaccgggta agagcaaaac ggagacctat gaggatcttg ccaaggataa
    13501 attcatcaag gagccggcca atatgcgcct gcttattgtg gtagataagc tgctgaccgg
    13561 atttgatgcg ccatcctgta cctatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag
    13681 cagttcgggt acattgttga ttacaaggat ctattcaaaa aggtggagaa tgcggtagcg
    13741 gtatatacct ctgagcttga ttatgacgaa ttcaagaaag aggattgtga tatcctgtta
    13801 aaagaccggt taaagaaggg gcgagaaagg cttgatctgg ctttggagga aatagaactg
    13861 atatgcgagg aagtgtctcc ccctaaatct aagctcgact accaacacta cttctgtggc
    13921 aatccggaaa tacaggatga gctgaaaaac accgagatgc gtcgtacggc tttgtataaa
    13981 gcaacagttg cgctgataag ggcttatgcc aatattacgg ctgaaatgga tgaagcgggg
    14041 tatactgaaa cggaacagga agacattaaa atgaaagttg acttctatat aaagctccgg
    14101 gaagaaataa ggcttgccag cggcgaaacg ctggnnnnnn nnnnnnaaga cctatgaggc
    14161 agatatgcgg cacctgattg ataactacat tcaggcagag gactccaggg ctatttcacc
    14221 attccaggat atgtcactta ttgatattat tgtcaagagc ggtattgctg aagcaatcag
    14281 taaaatgccg ggaggaatca agggcagcaa acaagctgtt tcagaaacga ttgaaaacaa
    14341 tgtccggcaa aagattatca aagaccacct gcttgaccct gcattcttcg aggaaatgag
    14401 caaactgctg gatgaaatca taaaagaacg cagagccaat gcgattgatt atgaagaata
    14461 tctcaaaaag atcgcagaca ttgctgccaa agtgcaatcg ggcaaatcag aaaagactcc
    14521 ggacgatatc aagacaccgg cacaaagggc tttgtataac aacctcggca agagtgaagc
    14581 gctggccctt aaactacatg atgcgatcct ccgtgtaaag cctgatggct ggcgtggcaa
    14641 cgaaccaaga gaaaatgcca tcaaggctgc cctgtatgaa atactgcagg acttcggaga
    14701 agtagaacgg atattcgata ttgttaagca acaaagggaa tactaatgga gaagatcagc
    14761 ctgggagata ttgtggtgga tgttgagcta aaggatatta agaatgtcca tcttagcgta
    14821 tatccaccca aagggagggt tcgcatagca gcgcccaggc gaatgtcatt agatactatt
    14881 cgcttatatg cgatatccaa attaggatgg attaggcagc agcaaaagaa gttccgggag
    14941 cagaccaggg aggcacctcg tgatttccta aacagggaga cacattactt tatgggtgtc
    15001 cggtacatgt tgaaagtggt ggagcatgat gccgctcctt ctgtacgaat caagcacaaa
    15061 tctctcgaat tatctgttcg tccgggtacg gggaaggcaa aaaggcagga aatcatnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnatta aaagaactgg taccgtctat tatctccaaa
    15181 tgggaaaaga agatgaaggt atcggtcagt gaattcggga ttaaacgtat gaaaaccaag
    15241 tggggcactt gtaaccggga agcaaaacgt atctggctga acctggaact ggcaaagaag
    15301 ccgctctctt gcatagaata catcgttgta catgaaatgg tgcatctatt agagagaaat
    15361 cacaatgacc gctttatttc atttatggac cggtttcttc cacagtggaa agagttgaag
    15421 aaggagctta acagattgcc ggtgagtcat cgtgattggg agtattgatt aaaacaatat
    15481 ttgaattgtt taaacttctt agcattagaa aaatgaaagc tacattcgaa gagaaatctt
    15541 ttgagaatta ctttaattca gagctcgaca aaaaggcatc gatctttttt ccgtttggac
    15601 aagttcagga aggcttttta ggttttgatg cctcttcttt ttcaaaaaat agaagtttgt
    15661 ggcgagcaat aggataccct tttttgtttt cgcctaaatt caagggggca gatttaatag
    15721 aaatagctga agaaatggaa aactacttgt ctgttacaat aaataacatg ccaaacttaa
    15781 aagcaaattt acttttccag tataaaaggc cagagtatgt taccagccca aaatgtgctg
    15841 aatggtactt atggaataaa ccatattatc ggtacaatat tgatatgaat cagcaattgc
    15901 tcttatcaaa acttcattca tctttttctg ggcgattact aattttgtac gtttcacccg
    15961 cttttcatga aataaacgac ttggtacatt ataaacaaaa aaataaaata atcaagaatt
    16021 caaatttcac aaaagcaatc gaccttaatg gtcattctaa aaatacatat ataactaaag
    16081 gcacatactc aatagcatgt agtgaaccta aacgcataaa gaaaattaac ctggagaatc
    16141 agcttgatgc tcttcgatct aatatatcta aatttgaaaa taacacacaa ttcatcaaag
    16201 actttagttc tcaaataaga gatatagttt cagaagatgc gacttattca aatgcattct
    16261 taaggctaaa ctccgaatac tcaatgctaa cagactatag tgtttttcat agtcttttgg
    16321 ttatggctaa ttttaaaaat cttacgggtg cacaatggat tattacaacg tgataaattc
    16381 aggacactat tgtacttaat aagccgaact ctataatata attataaata gctctttgca
    16441 tcaagtcttt acttcgtttt aaataatact agaaatttgg aacaaacaat gaataatacc
    16501 tcaagcaaaa gaaataatat ggcctattag atattattgc taaattagtg ataagagtat
    16561 atagattaag atgtgtagaa ttgaatcctt atttaataaa gaaccaattt tataatttta
    16621 tcaaaaaaag tgtcatataa tcgctattaa attaaataac caccaatagt tgaagaatgc
    16681 aaatattctc gttagataga gacaacgtaa ttaaagcata ttttttatct gggcgggtaa
    16741 cctataaata tgcaatagac aatctaatcc acttgattga taaattagac atccaacgca
    16801 aatttcaaaa cgaaacattt tataaacggc ttgaaattga tcttcaaaag ggttgtatac
    16861 ccccagccat aactatagca tttattaaag aaactgattt taataacccc atcacactta
    16921 atgaagctga acgacaaata aacgatgaca tttcaacagc ttttatactt gatggaatac
    16981 aaaggttgaa tacaattaaa cgaattgtat cacaagataa caatgaatta gatcttgaaa
    17041 gatatttgta cgttaacata ataatttgcc cctctaggga taacttatta tatagaatgg
    17101 tgacattgaa taacggccaa aaaccaatga ctgctagaca tcaaattgaa atattagctg
    17161 aaagcattta tgatttttct agctatcatt ttcaggtttt cactgaaaag gaaaatgctg
    17221 ataaaaaaga aaaaggattt aagaaggcag acattattaa tgcctacatt tcctttttaa
    17281 gcaattcaac aagtcttgat aataaaaaga taattgaaga taagcttgat gagttggtgg
    17341 ctaggaaaat tatagaatca aaaataacag acgattcagt tgattttaat aaagtactaa
    17401 aattagttgg ccgaatgtat gaatctgaaa gtttgtataa ttggttaaag aactcaaaca
    17461 atttgattgg cttttctgtc ggaattcgta attcttatat acacgtaaac gcattaaata
    17521 atgaagagtt agaaatacaa ctttctaagt tcgagaaagc ctttaagaat ataaatgtat
    17581 caaccataaa attaagtagg gagagaagaa ggctcacttc tttctttata aataaacttc
    17641 atgaccttat tgaaaaagat tcagacgagc tgacattgtt gcttgcagaa caaatctagt
    17701 cttacaatga gtctttattt attttctttt aaagatccaa ttccgccaaa ttacagatcg
    17761 gctgttcctc catattttcg agtaacaacc gctactactt atgatgttct cttgattcgc
    17821 ttattgactt ctactttgga tatttcaaac tccaaaacca ataaaaacat aaaactgact
    17881 agcaacttta ctgtttttcc agcatttacg gtttctagaa aatctgcaaa acaaatccgt
    17941 gcttttattt ctccctttaa tctgtcagat tatattaagt attctcagaa gtattatggc
    18001 agaaatataa ccttttacca caatctactt caagaactga cattctattt ttactactcc
    18061 agtgagaatc aatttcaatc tgcatttgta aatctatata gaacacttga gtatctttct
    18121 tatagctttc cgttgatgca tgcgtctcac ttcggaaact atattggatc ctttgaggca
    18181 ttaagagcat attttactga tgcaaatact agtgagttga aattctttga atcatttgtt
    18241 gcaagattat tccaagggac accttattta tcattaacaa caaattttga cttttcgcat
    18301 ccagatattc tggttgctaa taattgcttt gattcattta aaggcttaat gaaagctaca
    18361 gattggacca ctgctaataa acttactcat tctctttcaa ttgagaactc taaccttatt
    18421 aaacttttca aaaatgttag gaacagatat tttcattttg ctgtaggtgg ttaaagaaat
    18481 attcagaata ccgatttgaa agatccagat ttcttttttg agaaaattaa tgaccagttt
    18541 ttaaactgga ttggttttat ttatcaatca gtcattaaag aaaatctaga caatatatta
    18601 taggttcctt ttttatattt actttagact caatttgaga aaattgtgtc tttgattatc
    18661 aaaatatcta aatagacttc tcgatttaat agacctttcg aagatcactt aactattata
    18721 tcacctgatg cttttgatag aaatactctg taataaacaa aagcattata gataaaaaat
    18781 cccaacaagt taaaaggata taaaataaac agtgtttttc ccgcttcaat gtcattagaa
    18841 tcctcaattg aaagaataat acttttagtc agaataagtg ttaatagccc agtaataact
    18901 gccgttaaaa gaacgatgaa gatcttatgg ttctttgtag gtagcggcat ttcacacaaa
    18961 aagtaaactg atcccccaat taaaagaatt gcgagaacaa gtgaaatagc ccctggtaaa
    19021 atagtgtttt caaaatactc cgttgcactt cctccttgag gaacagatag actgccaagc
    19081 aaataaagtc caattaatag gaaaaagggt atgataatcc tttgcctaat gtttaactgg
    19141 cttacacttg agaatataat tattaataag agcgcgacgg gtaacaaata cttgtaaact
    19201 ctattaagaa taaggggtgt gtaactttcc tcccgctctc taatatttaa ttgaatgcgc
    19261 ctttcttctt ctttatcata cctttcttgc ctctccctta ttttatcttc cagattaatg
    19321 tctgtttcag ggaatggttg aattgaatta ctagtaccgt ttttaatctt tttagaattc
    19381 tgtgaatatg ctgagttgcc aagcaaaaaa agacataaaa atgaggcaag tacaactgaa
    19441 ccgaatttga ctattgtttt cataaaactt tacatgggcg ccgctaatca acttccatca
    19501 ctggggctcc gcgagggccc atagactaaa agtgaaagca gacgcccacg taaagcgggc
    19561 gtcttgcttt tctttcctct agtctatgct ggaatttcgc ggatttcagt gatgtcggaa
    19621 acaaaaacaa aacgtgttag ttcttcctaa gtcgttaagt catgcgctgg tgatttaata
    19681 agctaatata cagtaaatca gtctttcccc ccgatatacg gatacnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccc tacccgaaat cacacccgcc
    19801 ccttcaagga tgtgcttcta tcccctggta gaagccaatt attcattcac cttaaatctt
    19861 ttaaaaatgg taaaagttaa gcagtaccat gttcgccagg gtaatgatgg cgaaaagtac
    19921 atttctctcg aactggaagg cgatgtgtca tttgtgcaaa gccaaaactc cgggcggttc
    19981 tatgcgacca ccaagcgttg tttcatgtat gccgccatgg atgaaccaac tgccaaagca
    20041 ttggtaggga ctcaaatgcc gggaactatc gaaagggtgc cctgtgatcc gtacgaatat
    20101 accgtacccg aaacaggaga agtcaaagtg ctgtcgtaca cctacgaata ccggccggcc
    20161 gaagctgttg aattgccgga catgattggc tccgcgaggg ctaccacttt ggcttaacag
    20221 cggccagagt ccagaccaaa ggggtaggca caatgcctgc ccctcttttt taacttctaa
    20281 aaatcatgct atatgcaatt acaaaaagca tcccgcacta aggccaaaat gaaactggcc
    20341 ttacagggtc ccagcggttc cggtaaaagc atcggcgccc tgctcatcgc ttttgggctt
    20401 tgcggtaact ggagcaagat cgctgtcatc gataccgaga acagctccgc caacctatat
    20461 gctgatcttg gtaactacaa taccgtttcc atagggccgc cattcagccc tgagaaatac
    20521 atccaggcga tcaaactctg tgaggatagc cagatggagg tcatcatcat cgacagcgtt
    20581 tcccatgagt gggacggcgc aggcggtatc cttgacatcc actccaatat gtctggtaac
    20641 agttttacca attggggaaa gctcacccac cggcacaatg cctttgtcca ggccatgctg
    20701 cagagcccct gtcatatcat cagcaccatt cgttctaagc aggattacgt cctcaatgaa
    20761 aagaatggaa aaatggttcc tgaaaaagta ggccttaaag gtgtgcagcg cgacggttta
    20821 gattatgagt tcaccatcgt gctggacatc gatagtaagc ataatgccat agctagtaag
    20881 gaccggacat ctcttttcat cagtaagccc gagtttcaga tcactgtgga aaccggaaag
    20941 cagatcaacg actggtgtaa caagggagag gatgtaacag ccattgttac aaacagtata
    21001 acaaaggatg ttatatcaga agaacaacta ctccaggaaa tcaaaaactg caatacggtt
    21061 gacagcctac ttctcctata taacaaatac cccctttacc aggagaccca tgtaaaccag
    21121 ttcactgtcc gcaggaaaga actgctggcc tcgcagaatt caaaccccat ttctcatccc
    21181 ttaaaaccat cagccaatgg aactcataca agcgccactt aaccatcagt cggtcgtttc
    21241 tcctgcccct gaaacatatc tccctgcacc cgtcctctct catggaagcg ctttcattga
    21301 agccaatacc gtggaaagct ccctggagga aatcaaagaa aagcatatca tcccggtatt
    21361 catcaaggat aatgaaccgc tgatctctca cgctgacttt atctcggctg ctgaggaagt
    21421 agtatccgat gtgttcaatg gggaaacgat tctcaggccc aatatccgct tatcccatcc
    21481 gatcaagggc agaatacccg aggccaagga taagccggcc attcagctac aggaacatga
    21541 aaagacactg tattacgagc ggatggcttt tgtaatcgaa gtaccctcta tccacgatga
    21601 gatcgaaggc tctcagttat cgctgaccat tgggggtgta aagtccctga accaggataa
    21661 cctttataac aagaaaggag ccgatgagca ctttaaagtg tttatcggtt ttcagaataa
    21721 ggtgtgtacc aatctctgtg tgtggacaga cggatatatg agtgatctga aagtaaccac
    21781 cctggggcag ttacagggct gcatcaggac actggtcgat aactacaatg ccgtgtatca
    21841 tctgaacagg ctcaaagacc tgacacagtt tcagttgact gagcaacagt ttgccctgct
    21901 catcgggcgt tgccggatgt acccgcactt gcccaaacag atgcaatcag agatacagcc
    21961 gctgctcttt ggcgacagcc agatcaacac catagtgaag gattactacc gcgacaggtc
    22021 attctgccgc aatgaggacg gcaccatcaa tctctggaag ctgtataacc tgttcaccgg
    22081 ggccaataag tcgacctata tcgacaactt cctagacagg tcggtcaacg cctattcctt
    22141 cattgaacaa ctcaagtatg cgctcaaaaa ccacgatgat aactggttcc taaactaatc
    22201 tcttaaaaat ggaaaaagcc aactggatgc gttgggtgga tccttattcc atcctggtca
    22261 tcaatcagca gggaaagatc agaaggcttt actgtccgtt tcaagtaaag tgcatttccg
    22321 cggtcgaggg cataccagtc aatacctggt gttacgtgga cagggtttcc gagggtcgta
    22381 gggaaaagct gctgtatgat atcaacggcc gggagctgcc cttccatcac ttcgagattt
    22441 atgtaagatt ctgagcgcac cgagtattat caacccattt attcacccaa gcccccacct
    22501 caaaagtggg ggcttattaa ttaattcaac atgattgata tcgatcaggt ttcggaaata
    22561 tccattagat acaagcccaa gattgcaaaa cggcctgtgg ttaaatcctc caatgattgt
    22621 taccaggtcc tgaaatgctg gtttgatgag aacactatac atctgcaaga acaattcgtc
    22681 gtgatgtacc tgaaccaggg aaaccgggta ctgggaatgc tgaagctgtc aactggcgga
    22741 attaccggca cggtggcaga tatcaggctt attttggctg ttgccctgaa agcagccgcg
    22801 tgctctatca tcatcgccca taaccaccct tccggaaacc ttcagccatc caaggccgac
    22861 ctcgatatta cccggaaact caaagccgct tccgagctca tggacatccg gctcttggat
    22921 cacctgatac ttaacccgga taatgaatac ctgagcctgg cagacgaagg gcatatctaa
    22981 actctattta taacaacaat tattagcaat atgaacattt taataattct ggccctacta
    23041 tttatgtgct ggctgaccgg tttgatcact cggtctgtat ttggcgccga tcctgataaa
    23101 agtttagagg gattgggaaa taacctcctg aattttctga ttggtattct gatccaggta
    23161 ttgtggcacg tattctgacc ggggtcgtag caggcaaatt gaggtatatc ctgcgaatct
    23221 tctcaaatca ataacctact tttaacaata gctatggcaa aaagagtcga taactttaat
    23281 cttctgtaat ataccttaat gtttgatgta gaaaccaatt tgagatatcg atcagaaata
    23341 atattgcatc aatataaatc tatccgggag gtagacagaa ataaaaacaa aaataaatgc
    23401 atacttatat catgaattca aaacaaaatg ttgcctatct cgggatattg cggcggatat
    23461 ttgttacctt ttaatatttc aagaattccc aaaggattta tcactattcc aatatgtgaa
    23521 catttaagtt tattcaaatt ggaactcaca accttcgctt tttcctcact ttttcctaat
    23581 tgccaaatat gccaattgtt tgtgtaaagc agaggctggc ttccaacctt gcctccatag
    23641 tcatctgtgc ttctaaaaac tatttctaaa tccaatccaa ggtcgctatt gtagccttcc
    23701 tttttccaaa tattcatttt tattccctgg ttaacaaaac aatgtatgta agattccact
    23761 ccgctatctt ttaatttatc gatatccaaa ctttcatgag attcaaaatt tcccctgaaa
    23821 atgcgaataa catcgctatt caattgtaat ttatcaatgg aaatgtattg aaaatatctt
    23881 ttcttacttt tcgcgatttc aacagagaaa atatcaccaa tttttnnnnn nnnnnnnnnn
    23941 nnnnnnnntt attttgattt atttgctaat tccaaatatc gcccaatact ttggtgctat
    24001 tgaatttatc ttatttaata attgtccgcc gtttttcatc aatccatact gatttatcaa
    24061 tgtctgttca attattctag cttgttcttt agaaagacca acggcgcctg aaattacctc
    24121 ataatccagt aatgccttcg ccgaacttga attcagatgt tcattaaatc ttgctactgc
    24181 atctctactt gtaattccaa tatacctcac atttgcattg gcatctaccc ctttgtaaac
    24241 caaataagat ccacttttta actcctttgc agtttttctt gcaatctttg tgcctgtagc
    24301 tccccaccct gcaaatggaa tacaagcagc accactcaaa gcggcatttt cataatcccc
    24361 tctaagcgca taaattcctg cgtttagtcc atcaaataat tcgccaatgc ctggaacaag
    24421 tcctgcaata tcaaatccag tttgaacata atcaatcaga cttttctcat tatgtcgtga
    24481 aataccaatg aagttactcc ccattaatac ttcgccattt aaattaccac ctccattatt
    24541 gtaggattga ctaaatataa ttccaattcc taatatacta tgattccctt tatctctata
    24601 tcccccataa atttctttaa cctcgtaacc attataatat agtgttcctc ccccatttcc
    24661 atcatcgaca taattaaaca aattcataat cttctgaagc tcagtaaaat aagcgccaat
    24721 aaacgaatct ttaaatgtaa taatggtgtt ctgcaaagca ctccaactac ctccatgatc
    24781 ggtttcattc cataaataag aaattacatc atcgacattg ttgaaagaca gagaagcgcc
    24841 cgaaggatca ttaaaataaa tagggttatt tgcagagtaa ttataagggg taaaattcaa
    24901 gtaataatca gcaagaatat caatactcgc aaatcgtcct atctgagcat catattttct
    24961 caaataggta tcatagtatt cgattccttg ttcctcccac tcactaccgg cattatatgc
    25021 atagactgtt ttagccttta aaactgctcg ggagctgatt gcacccatga caagaccaaa
    25081 gggaaagtag ctcttttcct ccaccagggg gccagtgatg tgccgcaccg tgaggtcgtc
    25141 aaagtacaca tcctgcggac tttcat
//
LOCUS       DAORLS010000009        25157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1075_length_25157_cov_2.035017, whole genome shotgun sequence.
ACCESSION   DAORLS010000009 DAORLS010000000
VERSION     DAORLS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 25157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25157
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1075_length_25157_cov_2.035017"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK977_01545"
     CDS             <1..216
                     /locus_tag="PK977_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRF16813.1"
                     /translation="DEKKIRLYIGYSGWSEGQLDGEMKEKTWLTVKASRKLVFHNDHQ
                     EIWKDSLKHLGGDYEIMVNFPIDPQLN"
     gene            250..969
                     /locus_tag="PK977_01550"
     CDS             250..969
                     /locus_tag="PK977_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRF16814.1"
                     /translation="MRRLLLIPFAFLIMMLSGCSVLKNSGLIPTELEMALGLKDALSQ
                     GLFKGFDAFANPEGNPLVRFAFPGEAEKIEKTLRDLGFDKAINQVTGKFTRAMSSAVN
                     VARPLFFDAVKKMTIKDAAKILVTDNTHAATDYFKQAMKPGLMTAFRPIVDSTIKIEK
                     ADEEWKGITSIYNSIPFINKPLESSLTDFVSARVIDLMFLMVANEEEQIRSKYELRKT
                     DMMRKVFTWAEGEIKRKLQKN"
     gene            complement(1048..1305)
                     /gene="atpC"
                     /locus_tag="PK977_01555"
     CDS             complement(1048..1305)
                     /gene="atpC"
                     /locus_tag="PK977_01555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRF16815.1"
                     /translation="MNLEILTPEKKLYSGEVYGVQMPGISGSFEVLEKHAPLVSALKA
                     GRVKVLKDKQNHVANFDIQGGFVEVLNNRVTVLVEGATEIA"
     gene            complement(1336..>2581)
                     /gene="atpD"
                     /locus_tag="PK977_01560"
     CDS             complement(1336..>2581)
                     /gene="atpD"
                     /locus_tag="PK977_01560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRF16816.1"
                     /translation="KGRLFNVTGDPIDGLPAISKVGGRPIHGLPPTFENLSTATEVLF
                     TGIKVIDLIEPYAKGGKIGLFGGAGVGKTVLIQELINNIAKAYSGLSVFAGVGERTRE
                     GNDLMREMIEAGIMKYGDAFKHSMEEGGWDLSKVDMKELADSKATFVFGQMNEPPGAR
                     ARVALSGLTIAEYYRDGDGQGKGRDILFFVDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGIMQERITSTNNGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKIAD
                     LGIYPAVDPLDSTSRILSPLVVGEAHYNCANRVKSMLQRYKELQDIIAILGMDELSDE
                     DKLVVSRARRVQRFLSQPFHVAEAFTGLKGVLVPIEETIRGFNMIMDGEVDEYPEAAF
                     NLVGTIDDAIEKGKKLLAAAQG"
     assembly_gap    2582..2591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2592..2845)
                     /locus_tag="PK977_01565"
     CDS             complement(<2592..2845)
                     /locus_tag="PK977_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRF16817.1"
                     /translation="MANVGKVKQVIGAVIDVQFDGKLPEIYNALELKRENGDILVLEV
                     QQHLGEDSVRTIAMDGTEGIVRGTQVVDTGKAIAMPVGEG"
     assembly_gap    2871..2952
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3008..3559)
                     /locus_tag="PK977_01570"
     CDS             complement(3008..3559)
                     /locus_tag="PK977_01570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF16818.1"
                     /translation="MIFWPLIFSLNKGWTTDGQLCFGIESLNTRKLCNDTVLNERVQY
                     RPVDMNEIPDDLEGFDFNWSSCAFEHLGSIDKGLKFLKNQLKTLKPGGWSVHTTEYNI
                     SSNDETQDNNDTVIFRHRDIEHIVNELRKEGHFVEETDFSIGGLPEDYQVDVSPHRQD
                     VHVKLQVGSFVVTSLGIIIQKKG"
     gene            complement(3486..3827)
                     /locus_tag="PK977_01575"
     CDS             complement(3486..3827)
                     /locus_tag="PK977_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16819.1"
                     /translation="MPQREWQSTLVQDAGRRRTELCKAQDFQSSWYKWWCNELNEAPR
                     FHRKQWEFVYNMQALWERGCIMPGKRGLVFAVGTEPAPSIFAKYGCDILATDIFPEQG
                     MDYGWSAMLRH"
     gene            complement(3833..4837)
                     /locus_tag="PK977_01580"
     CDS             complement(3833..4837)
                     /locus_tag="PK977_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF16820.1"
                     /translation="MGTEIRELLKNDEMLEVAFYIPPAEKNSFGVDAKTIVEKRRFWD
                     FFNPVTKDCSVWHAPFQSGRIIPDKRKYPGIKILLTIHDLNPLHEGKSYEDQQKSIAH
                     TQVLIDKADALVCISDFTKSDVLTHCDVKDKPVYVIHNGTHKVHEPQLENSSYRPARP
                     FVFGMGYINRKKNYHVLLPLLENEGIEMVIAGRLDEPDYVNAMLQQAKKMGVADRLHL
                     PGPVSEGEKAWYFQHCMAFVHPSLAEGFGAPVVEVMQFGKPLFLSSYTSLPEIAGDTA
                     FYFQTFERNHLKWVFENGLAEYSKNGLAERIKQRGTLFDWKQKAKEYLALYKELAAIN
                     "
     assembly_gap    5273..5399
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5400..6207
                     /locus_tag="PK977_01585"
     CDS             <5400..6207
                     /locus_tag="PK977_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF16821.1"
                     /translation="TNYLPFRNKKIKVVLSIHDLNFLYDEKKSPAKKQKYLNHVQRLI
                     DRANAIVCISEFSKQDVLKQCNTGTKPLYTIYNGTNLLLHPELTPKSYRPKKPFLFSI
                     GVIHRKKNFHVLLPLLKKNTMELLIAGKPDDDHYVSLLKRESKKLGVEENVHLLGKVS
                     EKEKSWYYNNCSAFALPSVSEGFGLPVAEAMSCGKPLFLSRRTALPEIGGDVSFYFND
                     FNPDHMNEVFETGMKNYNCNGFRDRIQERGRNFNWDRAAKQYLEVYRSLY"
     gene            6226..8061
                     /locus_tag="PK977_01590"
     CDS             6226..8061
                     /locus_tag="PK977_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF16822.1"
                     /translation="MKVYRRLLTYARPFRWFVIPFFIFTILAVIFSVFQFALIIPLLN
                     VLFNPASGTEAAAAPAFSLSTSFFRDIFYYEVYRLKAINPVYALYFITSIIVLAVLLT
                     NLFRYLAQRTLIKARTLLVKRIREALFEKINHLHMGFFTKEHKGDLISRMNADVFEIE
                     GVAANSLEVLFKEPSLVIGYFVALFAISTQLTLFTLFIIPISALGIAFVQKKLKRDAK
                     DAQASIGRLLTIMDETLTGMRIIRAFNATSFTLKKFSKENDFYRNASLQGFKRREMAP
                     AFSEAAGVMVVAGILLYGGSLILKNGQSAAGIQASEFIAFIAIFSQVLRPAKAMVIAG
                     ANIQRGRGAGERILEIIDKPIEVTDKEDAVELKAFKHSVEFRNVHFSYNDTPVLIDIS
                     FTIPKGKTIALIGASGVGKSTIADLIPRFYDVKSGAVFIDGVDVRDYKMESLRSVMSF
                     VTQEIFLFNDTIFNNIALGRPDATEEEVIAAAKIANAHDFILQTENGYQTITGDRGIR
                     LSGGQRQRLCIARAVLKDPSILILDEATSALDTESERAVQEALSQLMKGRTTLVIAHR
                     LSTIKEADEIIVLHDGRIIERGHHNELIEIDEGVYKKLTQMQQIA"
     gene            8143..>9158
                     /locus_tag="PK977_01595"
     CDS             8143..>9158
                     /locus_tag="PK977_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16823.1"
                     /translation="MRLIIITFLIVFHTFVNAQSVRLLFAERKYTDIIKEYGNKESDL
                     DGEETYMLGYSYFHSGNDEKTIIALDKALKKGFDEPVVYFFKGIAFRYLDKIPEAMEA
                     FDSACFRDNQNQEYACERAFTLYYIKKYDEAIAAYEEAKKLPNTYQAPWYMIPHILLL
                     QKKDDEALMGFYNGLSNISKDNQFYSSSLMDIGMIQNRLKKNYGKAIEAYMQILKENG
                     FDYAALEGLMYVSYKSGNKQKADSIFLILKKAFEEKKLQVNMMKYKRAKIAEFDIEGK
                     EVFVYKSFIPPAKMIDIIYTGYVMSNGGEKMERIFQTEQTIQLTDNSPKHILCEVWTD
                     GKGRS"
     assembly_gap    9159..9169
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9346..>10088
                     /locus_tag="PK977_01600"
     CDS             9346..>10088
                     /locus_tag="PK977_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16824.1"
                     /translation="MQLTEVTTPQLARDFIRVNVELYKDDPNYFRPLDKDINEVFDAS
                     KNKAFRFGEAVRWILKNDQGKLIGRIAAFVNKKYKNKGDDVPVGGIGFFDCINDQLAA
                     DMLFDVAKHWLMQKGMQAMDGPINFGERDRWWGLIVEGFQPPLYCMNYNKPYYKELFE
                     RYGFKNFFNQICFGLDPKKELSQKILDRHAQLKENPAFRSSYINKKQLEKFAADFTIV
                     YNKAWAGHGGLKELKKEQVLTMFKKMKPV"
     assembly_gap    10089..10116
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10117..10519
                     /locus_tag="PK977_01605"
     CDS             <10117..10519
                     /locus_tag="PK977_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16825.1"
                     /translation="VYHNQDPIAIFVNLPDLNQWFKYLNGKFDLWHKLKFLWIKKTRK
                     NHRFTGLVFGIVPEFQGMGVDAFIINESKFVIQPQTDYTEYEMQWIGEFNPKMINVVE
                     NFGDTFRTRKLVTYRYLFDQTKEFKPHPVIL"
     gene            10583..>11616
                     /gene="dnaX"
                     /locus_tag="PK977_01610"
     CDS             10583..>11616
                     /gene="dnaX"
                     /locus_tag="PK977_01610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRF16826.1"
                     /translation="MDKFIVSARKYRPQTFDTVVGQSHITTTLKNAIKHQQLAHAFLF
                     CGPRGVGKTTCARILAKTINCENPTKDGEACDKCQSCKSFSEGASMNYFELDAASNNS
                     VDNIRDLVDQVRFVPQTGKYKVYVIDEVHMLSSSAFNAFLKTLEEPPSYAIFILATTE
                     KHKILPTILSRCQIFDFKRITTNDTVEHLQEICEKEEIKADKAALQVIAQKSEGCMRD
                     ALSILDKIVSFTNGELNYGNTLEHLNILDAEVYFKLLDCMEQQDLSAALLLYDEINRK
                     GFEGDLVLNGFAEFIRNLLVCKDEKAAGLLEVVESFKDKYITTGKKISAALLISALNI
                     LNDAELNYKAA"
     assembly_gap    11617..11626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11627..12409
                     /locus_tag="PK977_01615"
     CDS             <11627..12409
                     /locus_tag="PK977_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRF16827.1"
                     /translation="NKRLHVELALIKLCYLQQAIELTADGNAVSKKKRVDAVKPVAFR
                     SINPFIPVTAENNTGDTENSAKLVIETPSLSSVQEPVPGYRQTGTPPDQQGGSNEQPG
                     TSNQLTATGKKLTALNKIRQQYQNNGNNGNTVNHPLQLEELQKAWADYVVKLKDAKNP
                     AAQPFELAVLRIRDENSFEAITANSIEKQFIEQDRNKLFVYLRERLQNRLLQFSVTVE
                     EKAENRPVIEIPLSASEQYNKMVELYPLVKELKDKLNLTLDY"
     gene            12491..14110
                     /locus_tag="PK977_01620"
     CDS             12491..14110
                     /locus_tag="PK977_01620"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HRF16828.1"
                     /translation="MAEVILMPRLSDTMTEGVIAAWHKKVGDTVKKGDLLAEIETDKA
                     TMELESYKDGTLLHVGIDKGGKLQVDDLLAIVGAPGEDISALLGSTKTATKKEEPAQT
                     PSPSATASTPATTQALDISKMDEVVLMPRLSDTMEVGVIAAWHKNVGDTVKKGEVLAE
                     IETDKATMELESYKNGTLLHIGAQKGEKIPVNGLLCIIGEKGKVDVDAIVAAVNAGMV
                     TTNAAATPDDSQPALAEASTTTAVTTQPATASSENGRVKASPLAKKLAAEKGIDISTV
                     SGSGDGGRIIKADIDKYKPSATAAPAAKAPAFTVTGQEGYTDIPNSQMRNVIAKRLGE
                     SKFSAPHFYLTMEINMDNAMVARAQLNELSPVKISFNDMVVKAVALALRQHPAINSSW
                     LGDKIRRYSHIHIGIAVAIEDGLIVPVVRFADQKTLPQIAAESKELAGKAKNKKLQPN
                     EFTGNTFTISNLGMMDIDEFTAIINPPDSCIMAVGRIKEVVVRKGDGFGVSNVMKVTL
                     SCDHRSVDGASGAAFLQTFKKYLENPITMLL"
     gene            14314..14535
                     /locus_tag="PK977_01625"
     CDS             14314..14535
                     /locus_tag="PK977_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16829.1"
                     /translation="MEDRDLNGFSWQTLFFVTGISFVLFSLVLKFLDGAHSVPMLWIG
                     AISLSLGLIIWAIDMAIRTPVRKNGKYSS"
     gene            complement(14537..>14992)
                     /locus_tag="PK977_01630"
     CDS             complement(14537..>14992)
                     /locus_tag="PK977_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRF16830.1"
                     /translation="VASDNIIYVMAPDRVVSAIDAVNGKTLWRSKDGGVRESIGISAD
                     GKWIYGKSMQDTIVAYAASREQQSAAWKLHAGFGYEHVPSMLIEKDGHVFFGTKSGVV
                     YCIDPTEKKVVWAHKIDNSMVNTVRVLSKSQLVVSTMDGKLVLLNISGS"
     assembly_gap    14993..15002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15003..>15994)
                     /locus_tag="PK977_01635"
     CDS             complement(<15003..>15994)
                     /locus_tag="PK977_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRF16831.1"
                     /translation="VRMSDGHVPRDAVNWLDKELKKVKKNQPVIFLNHYPIDKGLDNW
                     YEITDRLRKHNTWAILCGHGHANKAMNFEDIPGVMGRSNLRAKDNEGGYNLVDVRNDS
                     ILFTVRRPVSGNERKWTGVKIESRNYEQEKKFPRPDYSINDKYGQVKKAWEFSSDANI
                     ISTPAVANERVFVGNQNGVMSCFSLKDGKPLWKFRTEGSIFSSPAVANGKLVFGSGDG
                     NVYCVDVNKGKLIWKYKTAASVLGSPVINGDTVFIGGSDHSYLALSSKNGQLIWKFAG
                     LEGPVVSTPLLYQDKIVFGAWDRHLYALNRSNGNLLWKWNNGSSVRNYSPASCI"
     assembly_gap    15995..16069
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16070..16396)
                     /locus_tag="PK977_01640"
     CDS             complement(<16070..16396)
                     /locus_tag="PK977_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF16832.1"
                     /translation="MKKYFLLFLLGFSLTAFSQVKSFRFAFISDTHIGSPNGSAEEDL
                     RRTVRDINAMSDVAFVVLTGDITELGTNEEIKLARQILDSLDIPWYIIPGNHDTGWSE
                     SGGVMFT"
     gene            16524..17117
                     /locus_tag="PK977_01645"
     CDS             16524..17117
                     /locus_tag="PK977_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16833.1"
                     /translation="MILLDISSWWQALTSMEKIFWGISLLFSALFLIQLVLSFIAGDG
                     DSSLGDADEYISGDEGMGHQFFTIKNLIAFFTIFGWTGIACLKGGMSKGIAIAIAVLA
                     GSLVVIIMAFLFKSMSKLKQSGTLQIKNAVGLIAETYLFIPPKRGGFGKVHVKVQGSL
                     HELQAITDDEEQIATGKLVKITAVINDAVLLVTSKLS"
     gene            17139..>18164
                     /locus_tag="PK977_01650"
     CDS             17139..>18164
                     /locus_tag="PK977_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRF16834.1"
                     /translation="MAENLLLVVAAAILFFFILVYSMFRRYKRCPSDRILVVYGKVGK
                     GAEGTQTAKCIHGGAAFIWPIIQDYSFLDLTPISIEVNLTSALSKQNIRVDVPSRFTV
                     GISTEPAVMQNAAERLLGMQRPQIHDLAKDIILGQMRLVVAMMDIEEINNNRDKFLAN
                     IASNVEAELNKIGLKMINVNVTDIKDESGYIDALGKEAAAKAINEAKIRVAEQERTGD
                     IGKTTAEKERDIKVAETQRDRDINVALALKEKEIQIAGANRDENIGKAEAERDTRVKM
                     AEANAIAVQGENLSKIEIANSDAARREREAEALKKAIAAEKVQAARALEEAYLAEQKA
                     ENARAERD"
     assembly_gap    18165..18226
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18227..18701
                     /locus_tag="PK977_01655"
     CDS             <18227..18701
                     /locus_tag="PK977_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HRF16835.1"
                     /translation="KQKLIIEAQAAAEQLREKAKGEADAIFAKLEAEARGMYEILTKQ
                     AEGLERIVKSAGNNPKDAVLLLIADKLPELVKMQTEAIKNIKIDKVTVWDGGGQGADG
                     KGSTANFMSGLYKAVPPLKDIFDMAGMDLPTYLGKKKEEAAVEVKAEEVKEEKKK"
     gene            18725..19483
                     /locus_tag="PK977_01660"
     CDS             18725..19483
                     /locus_tag="PK977_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF16836.1"
                     /translation="MKKITILLSIFFIYTTVFAQKGKSGNISYYVFDAAWKPCNTDDA
                     VYFAILEKLNDTAWQWKNYLFSGPLLSVETYKDEDGTIPHGYFAWFDSNGNIDSSGET
                     FGGRKHGSWYYFTDSLSVWQTEKYENGILSERKDSILLRNEKRSNDSIGYLPGDKEAV
                     FKGGTNAWIKYLQNNIKIPGRAEKMGKSGTVKIFFMVDSDGSLKDLRLYKSVEYSLDE
                     EALRLIRKSPKWEPARQMDKPVKAYRIQPITIVF"
     gene            19537..20043
                     /locus_tag="PK977_01665"
     CDS             19537..20043
                     /locus_tag="PK977_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRF16837.1"
                     /translation="MKLTEAKQQFISSWGAFGTNWGINRTMAQIHALLLVNPDPLTQD
                     DIMEELNISRGNTNMNIRELINWGLVERVLLTGERKEYFTAEKDIWKVVKQIVKERKK
                     RELEPMLQLLDKLEEVEGDKRDKHVKTFVDTVSSIKKLGRQADKTLDTMIKAEESWFI
                     GSLMKLFK"
     gene            20117..20506
                     /locus_tag="PK977_01670"
     CDS             20117..20506
                     /locus_tag="PK977_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16838.1"
                     /translation="MKLLKLTDLVLQLLITISGIVLGIIKDDIQILLYLYFILGGWQL
                     ASYLTHYLTGVSWANRVERSQYGHTVLWVAVVGVLCYLLLLADLPFIAFYLLAMVIIS
                     PVMACWYFVIGLAEWRTIRHRELIHLK"
     gene            20528..20893
                     /locus_tag="PK977_01675"
     CDS             20528..20893
                     /locus_tag="PK977_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRF16839.1"
                     /translation="MKAKTTLVLGASDNPSRYSYLAIKRLRNHGHPVFAIGRKHSFVD
                     DTEIMTEKKQLEGIDTVTLYLNPQHQQDYYNYILSLHPKRIIFNPGAENDELAELATL
                     KGIQTIDACTLVMLSTNQY"
     gene            21092..21565
                     /locus_tag="PK977_01680"
     CDS             21092..21565
                     /locus_tag="PK977_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16840.1"
                     /translation="MKNRSFIALCVSALIFNVQLLAQNQDVPLNEPDYNKPLLFRSFP
                     DSIPVNIASFDRAVSFSQGEDVSVNISDNVHFPFPGKVMSIASKYGNSIQSVVIKSNV
                     YDGAGLTISKVTDSNGNIFYTGRIVSMKSGDLFELVKTESGYFLVKRKFYDLINE"
     gene            21634..23232
                     /locus_tag="PK977_01685"
     CDS             21634..23232
                     /locus_tag="PK977_01685"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HRF16841.1"
                     /translation="MKNYLQSCLMALFLFGAVKSNAQVPVYSSLPSATAVVFLDFDGH
                     TVNGTSWNYAGPIYCGASGMNSTQITEVFNRVAEDYRPFNVNVTTDSTKYLAAPANRR
                     MRVILTVSYEWYGAAGGVAFVNSFTWGDDTPCFVFTSLLNYNTKNISEASSHEAGHTL
                     GLYHQARYDANCVKQSDYHSGAGAGEIGWAPIMGVGYYQNLTLWNSGPNPYGCNNIQN
                     DLTVITTNNGFSFRTDDHTASFPTATNATFVNNQFTVSGVITQNTDQDLFRFTKPTAG
                     RFQLSAIPYNVGTGNSGSNLDLQITLFDGAQNQISVYNPGTLLSSVIDTLLNAGTYYL
                     RVEGKGNMYAPNYASLGSYSLQGSLTAGGTLPLRRLELTGELNGDKHRLNWLIDADEQ
                     VVEQVLEVATDGRNFSPVVNPGNSDRTFMYRPYVSTSAQYRVFVTFDNGKSYYSNVVT
                     LKGTGTVEKPRLVTNLVSNNLITVTSPGVFGYTVVDMNGKVTARGQLTNGLNNIPASF
                     MNGGMYIIRFVNGNEQWTDKFVKQ"
     gene            23322..23795
                     /locus_tag="PK977_01690"
     CDS             23322..23795
                     /locus_tag="PK977_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HRF16842.1"
                     /translation="MRWRKFNGDPIDLPIKDAVENAIKRETEKGFRLKVCIGTDSQVK
                     GQETEFATVIVFLREGHGGFMFIHNEKTRQQFTIKERMLMEVAKSIEIAYELCNLFTV
                     YDVDMEVHADINTNPNFKSNDALKEAMGYILGMGFAFKAKPEAFASSSCANKIVN"
     gene            complement(23850..24185)
                     /locus_tag="PK977_01695"
     CDS             complement(23850..24185)
                     /locus_tag="PK977_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16843.1"
                     /translation="MKRFLISLLLILPLFVDAQATDKIRKGDIKQQMLNKDSVAQKIQ
                     RFKDSIAKKIDRQQVAINIQNNVDQLLEIQKENKDRQRSAAITRLAIGAAFLALLVMG
                     LMKRRKKQG"
     gene            complement(24214..24405)
                     /locus_tag="PK977_01700"
     CDS             complement(24214..24405)
                     /locus_tag="PK977_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16844.1"
                     /translation="MKTILSERNIVVVLFIMVLVTFSLAQEDSKKIEKMYSGVQAQNA
                     RFLVQQDPVIPAAGLPSVK"
     gene            complement(24479..25096)
                     /gene="yihA"
                     /locus_tag="PK977_01705"
     CDS             complement(24479..25096)
                     /gene="yihA"
                     /locus_tag="PK977_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRF16845.1"
                     /translation="MEIISATYIISSPGWKQCPPADKQEYAFIGRSNVGKSSLINMLT
                     NKKGLAKISATPGKTQLINHFEVVSSDKKKWYLVDLPGYGYAKVSQKSRNNWGNMIES
                     YIRKRENLVCLFVLIDSRHEPQKIDIEFINQLGEWQVPFVLVFTKADKNKPGATKRNV
                     AAFLTALADNWEEYPPYFVTSALTREGRDLLLNYIRDFNAGVSKA"
BASE COUNT         7521 a         5296 c         5143 g         6782 t
ORIGIN      
        1 gatgaaaaga agatccgttt atatatcggc tattctggct ggagcgaagg gcaactggac
       61 ggtgaaatga aagaaaagac atggctgacc gtaaaagcaa gccgcaaact tgtttttcat
      121 aatgatcacc aggaaatatg gaaagattcg cttaaacatc tgggaggaga ttatgaaatt
      181 atggtcaact ttccgatcga tccgcagctt aactgattca cttctgatat tacaaaaaaa
      241 ccggttttta tgagacgcct tttacttatc ccatttgcat tcctcatcat gatgttgtct
      301 ggttgcagtg tgttgaaaaa ttccggcctc attccaactg aactggaaat ggcactcggc
      361 ctgaaagatg ccttatcaca gggattgttc aaagggtttg atgcttttgc caatcccgaa
      421 ggaaatccat tggtacgttt tgcctttccc ggggaagctg aaaagattga aaagacgtta
      481 agagaccttg gctttgataa agccattaac caggtgacag gcaaattcac ccgggccatg
      541 tcatctgctg taaatgtagc caggccacta ttttttgatg cggtaaagaa gatgacaata
      601 aaagatgctg cgaaaatttt ggtaactgac aatacacatg cagctaccga ttatttcaaa
      661 caagccatga agccgggact gatgacagcc ttccggccta ttgtggacag caccattaaa
      721 attgaaaaag cagatgaaga gtggaaaggt ataaccagta tttacaacag cattcctttt
      781 atcaacaagc cccttgaaag cagtttaact gatttcgtct ctgccagggt aattgatctt
      841 atgttcctga tggtggcaaa tgaagaagaa caaataagaa gcaaatatga gttgagaaaa
      901 acggatatga tgcgaaaagt atttacctgg gcggaggggg aaataaaacg aaaacttcag
      961 aagaactgaa aacaaaaaac cctcttctta gaagagggtt cataatttgt tttcaaaatc
     1021 cggacacctg gtggtttttc cagttcgtta tgctatttca gttgctcctt ctaccaatac
     1081 agtcacccgg ttattcaata cttctacaaa accgccctgt atatcgaaat tagccacatg
     1141 attttgtttg tctttgagca cttttacccg acctgccttt aatgcactta ccaacggggc
     1201 atgtttttcc agtacttcaa atgaaccact gatgcccggc atctgtacac cgtacacttc
     1261 gccactgtag agcttttttt ccggagttaa tatttctaag ttcatggtta ctcgtttctg
     1321 gttctggtaa cttgtttagc cttgtgcagc tgctaataac ttcttccctt tttcaatagc
     1381 gtcatcaatt gtacctacca ggttgaaagc tgcttccggg tattcatcta cttcaccatc
     1441 catgatcata ttgaaaccac ggattgtttc ttcgataggt accagtactc ctttcaatcc
     1501 agtaaaggct tcagccacat gaaatggttg agaaaggaaa cgctgcaccc ttctggcacg
     1561 gctcactacc agtttatctt catcactcaa ttcatccata ccaaggatag caatgatatc
     1621 ctgcaactct ttataacgct gcaacattga tttcaccctg tttgcacaat tgtaatgcgc
     1681 ttcgcctact accagtggtg aaaggatcct tgaagtagaa tccagcgggt caaccgcagg
     1741 atagatacct aagtcagcga tctttcttga taatactgtt gtagcatcca ggtgagcaaa
     1801 agtagtagca ggtgctgggt cagtcaggtc atccgcaggt acgtaaacgg cctgtacaga
     1861 agtgatagaa ccgttattgg ttgaagtgat tcgctcctgc atgataccca tctcggtagc
     1921 cagcgttggc tgataaccca ccgctgaagg catacggccc agcaaggctg atacctcaga
     1981 accggcctgg gtgaaacgga agatattgtc aacgaagaaa aggatgtcac ggccttttcc
     2041 ctgtccgtca ccatcacgat agtactcagc gatagtcaaa ccagacaaag ccacccttgc
     2101 cctcgctccg ggaggctcgt tcatctgtcc aaatacgaaa gtggcttttg aatctgccag
     2161 ttctttcata tccactttgc tcaaatccca tccgccttct tccatgctat gtttgaaagc
     2221 atcaccatat ttcatgatac ctgcttcgat catttccctc atcaggtcat ttccttcacg
     2281 ggtcctttca cctacaccgg caaatactga taaaccagaa taggctttag caatattatt
     2341 gatcagctcc tggatcaata ccgttttacc cacaccggca ccaccaaaca gaccgatctt
     2401 acctcccttt gcatagggtt cgatgaggtc gattacttta ataccagtaa aaagtacttc
     2461 tgtagcggta ctcaggtttt caaaggttgg aggtaatcca tgaataggac ggccacccac
     2521 tttgctaata gcaggcaatc catcaatagg atctccggtt acattgaaca aacgtccttt
     2581 gnnnnnnnnn nataccttca cccacaggca tggcaattgc ttttccggta tcaactacct
     2641 gtgtaccacg aacaatacct tcggtaccgt ccatcgcaat ggtacgaaca ctatcctccc
     2701 ccaggtgctg ctgtacttca agcaccagta tatcaccgtt ttctcttttc aattccaaag
     2761 cgttgtagat ctcgggaagt ttaccatcaa actgtacgtc gataaccgca ccgattacct
     2821 gttttacttt accaacattt gccatataag gagtttaaat aagaggtttg nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nngggttgaa aaacggtcct aattccttgt caggcaaaga gaaaatgatg
     3001 tctccgccta ccctttcttc tgaataatga tacccagtga agtaaccaca aaactgccta
     3061 cctggagttt gacatgaacg tcttgacggt gagggctgac atccacctgg tagtcctccg
     3121 gcaatccgcc aatggaaaaa tcagtttctt ctacaaaatg tccttctttt ctcagttcat
     3181 ttacaatatg ttcgatatcc cggtggcgaa aaatgacagt atcattatta tcctgcgttt
     3241 catcattaga agaaatatta tactcagtgg tatgaaccga ccaaccgccg ggttttagtg
     3301 ttttcaactg gtttttcagg aacttaaggc ctttatcgat agaaccgagg tgctcaaaag
     3361 cacaacttga ccagttaaaa tcaaaccctt caaggtcatc cggaatttca ttcatgtcta
     3421 ccggcctgta ttgaactctt tcgtttagaa ctgtgtcatt gcataatttt cgggtattca
     3481 gggattcaat gccgaagcat agctgaccat ccgtagtcca tcccttgttc agggaaaata
     3541 tcagtggcca aaatatcaca cccatatttt gcaaaaatcg acggagcagg ttctgttccg
     3601 acggcaaata ctaaccctct tttacccggc atgatacatc ccctctccca caaagcctgc
     3661 atattgtaaa caaattccca ttgcttccgg tgaaaccgcg gagcttcatt caattcgttg
     3721 caccaccatt tataccagct gctttgaaaa tcctgtgctt tgcacaattc ggtccttcgc
     3781 cttccggcat cctgaacaag ggtcgattgc cattctcttt gcggcatttg atttaattta
     3841 tagcggccaa ttctttatat aatgccaggt actcctttgc tttctgtttc caatcaaaca
     3901 atgtgccccg ctgcttgatt ctttccgcca acccattctt tgaatattcg gctaggccat
     3961 tttcaaaaac ccattttaga tgattccttt caaatgtttg aaaataaaaa gcagtatcac
     4021 cggctatttc tggcaacgat gtataagaag aaagaaaaag aggttttcca aactgcatta
     4081 cctctaccac aggtgcacca aatccctctg ccagtgaagg atgaacaaaa gccatgcaat
     4141 gctggaaata ccaggctttc tctccttctg aaaccggccc cggcagatgc aggcggtctg
     4201 ctactcccat tttctttgcc tgttgcagca ttgcattgac ataatccggt tcatctaatc
     4261 tgcctgcaat caccatttca atgccttcat tctccagtaa gggaagcaat acatgataat
     4321 tcttttttcg gtttatatat cccataccga aaacaaaagg tctcgcaggc cggtaagaag
     4381 aattttccag ctgcggttca tgtaccttat gtgttccgtt gtgaatgaca taaacgggtt
     4441 tgtcttttac atcgcaatgc gtcaatacat cacttttggt aaagtcagaa atacaaacca
     4501 gggcatcggc cttgtctatg agtacctggg tatgcgcaat acttttttgt tgatcctcat
     4561 aagatttacc ctcatgcaaa ggattgagat catgaatagt gagcaaaatt ttaatccccg
     4621 ggtattttct tttatcgggt atgatccgac ctgattggaa tggcgcatgc catactgagc
     4681 aatctttagt taccgggtta aaaaaatccc agaatcttct tttctcaaca atggtttttg
     4741 catcaacgcc aaaggagttc ttttctgccg gtggtatata gaatgccact tcaagcattt
     4801 catcattttt caaaagctcc ctaatctcag tacccatgtt gagacaatag tggtacaacc
     4861 cggaatgtgg gtgcttcatg aggtcgcaat ccagtattat tcttttcttt cttcctgcca
     4921 tataaaacca atcttatcag actttagtgc aacttaagca ttcggatgta atctcccatt
     4981 gacaaaaatc ttaccactgt gttttgttac atttaagcga actgtgttta caaacgttga
     5041 agaatcatac tgccgtgtga ataatatcaa ctggtatata ccgcaattag tatgattttt
     5101 gaaacaataa gtaataccca aaaacgtaca atgcgccgga ttgtatttga ttgtgaaagg
     5161 atgaaatatc ctgatacggg actctatcat tattgcctga acctggggaa acacctggaa
     5221 aaaaacatca acctgcagga ggaaaaactg tttttttata cccctcccta tgnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5401 accaactacc tcccctttcg caataagaag ataaaagttg ttctttccat ccatgacctt
     5461 aactttctgt acgacgaaaa gaaatcgcct gccaaaaaac aaaaatacct gaaccatgtg
     5521 cagcgtttaa ttgaccgggc caatgccatt gtttgcattt cagagttcag caaacaggat
     5581 gtgctgaagc aatgcaatac cggcaccaaa cctttatata ccatttacaa tggtactaac
     5641 ctgctgctgc atcctgaact caccccaaaa tcatacagac ccaaaaaacc ctttctcttt
     5701 tctattggtg tcatccatcg aaaaaagaat ttccatgtct tgctgccact actaaaaaag
     5761 aacaccatgg agttgctgat agctggcaag ccggatgatg atcactatgt atcactctta
     5821 aaaagagaat caaaaaaact gggcgtcgaa gaaaatgtgc atttgctggg aaaagtatct
     5881 gaaaaagaaa agtcatggta ttataataat tgcagtgctt ttgctttacc ctccgtttcg
     5941 gaaggatttg gattgccggt agccgaagcc atgagttgcg gcaagccctt gttcctgtcc
     6001 cgcagaacag ccctgccgga aattggaggc gatgtttctt tctactttaa tgattttaat
     6061 cctgatcaca tgaatgaagt gtttgaaaca ggaatgaaaa actataattg caatggattc
     6121 agggaccgga tacaggaaag aggcagaaac ttcaattggg acagggctgc caaacaatac
     6181 ctggaagttt accgctcatt atattaaata tctttaccgg tacaaatgaa agtttacaga
     6241 agactgctca catatgcccg gccgttccgg tggtttgtga tacccttttt tattttcact
     6301 attctcgccg ttatcttcag cgttttccag tttgccctga tcattccatt gctgaatgta
     6361 ttattcaatc cggcatcagg cactgaagct gcagcagcac ctgctttttc gctttccacc
     6421 agtttcttcc gtgatatttt ttattacgaa gtgtacaggc tgaaagccat taaccctgtt
     6481 tatgcgttat attttatcac ttccataatt gtactggctg ttttactcac caacctgttt
     6541 cgctacctgg cacagcgcac attgataaag gcaagaaccc tgctggttaa acggatcagg
     6601 gaagctttgt ttgaaaagat caatcacctg cacatgggct tttttaccaa agaacataaa
     6661 ggagacctca tttcccggat gaatgctgac gtattcgaga ttgaaggggt ggcggctaat
     6721 tccctggaag ttttattcaa agaaccctca cttgttattg gttatttcgt ggcgctgttc
     6781 gccatttcaa cccagctcac actcttcacc ctgttcatta tacctatatc cgcattgggc
     6841 attgcatttg ttcaaaagaa attaaaaagg gatgccaaag atgcacaggc ttccattgga
     6901 cgtttgctga cgatcatgga tgaaacatta acaggtatgc ggatcatccg ggcatttaac
     6961 gctaccagtt ttaccttaaa aaaattcagc aaggaaaatg atttctacag aaatgcaagt
     7021 ttacagggct ttaagagaag ggaaatggcc cccgccttct ccgaagcagc aggtgtgatg
     7081 gtggtagccg gtattttgct ttatggagga agcctgattt tgaaaaacgg gcaaagtgct
     7141 gccggcatac aggccagtga gtttatagct tttattgcca ttttctcaca ggtattaaga
     7201 cccgccaaag ctatggtaat agccggggcc aatattcagc ggggccgcgg ggccggcgaa
     7261 agaatactgg agatcattga taaacccatt gaagtaacag ataaagaaga tgcggttgaa
     7321 ctcaaagcct ttaaacattc ggtggaattc agaaatgtac atttctcata taacgataca
     7381 cctgttttaa ttgacataag ttttaccata cccaaaggaa aaaccattgc tctgatcggg
     7441 gcttccggtg tgggcaaatc aaccattgca gacctgatac cccggttcta tgatgtaaaa
     7501 agcggggcag ttttcattga tggtgtggat gtgagagact acaaaatgga atcgctgcgt
     7561 agtgtgatga gttttgtaac ccaggaaata tttctcttca atgatacgat atttaataac
     7621 atagccctgg gtcgcccgga tgcaacagaa gaagaagtaa tcgctgctgc caaaatagcc
     7681 aatgcccatg atttcatact gcaaacggaa aacggctatc aaaccatcac cggcgaccgg
     7741 ggtattcgtt tatcaggcgg gcaacgtcag cgattgtgta tagcaagggc tgtactgaaa
     7801 gatccttcca tccttatatt agatgaagct acttctgccc tcgatacaga atcggaaaga
     7861 gccgttcagg aagcactaag tcaactcatg aaaggcagga ctactctggt cattgcccac
     7921 cgcctaagta ccatcaaaga agcagatgaa ataattgtac tgcatgacgg aaggataatt
     7981 gaaagaggtc atcataatga attgatcgaa atcgatgaag gtgtctacaa aaagctaaca
     8041 caaatgcagc agatagcctg atgtattacc tggatataaa aaaggaattc agtattatgt
     8101 attcaaaacc gaattttggt aacttaggta aacccttgcc aaatgcgtct tataataatc
     8161 accttcttaa tagtatttca cacttttgta aatgcccaat ctgtacggct gctgttcgct
     8221 gaacgcaaat ataccgacat tataaaagag tatggtaata aagagtcgga tctcgatggt
     8281 gaagaaactt atatgcttgg atatagttat tttcattcag ggaatgatga aaaaacaatt
     8341 attgctttag acaaagcttt gaagaaagga tttgacgaac ctgtggttta cttctttaaa
     8401 ggcattgcct ttcgttacct tgataaaatt cctgaagcta tggaggcatt tgattcagca
     8461 tgctttcgtg acaatcaaaa ccaggaatat gcctgtgaaa gagctttcac attatattat
     8521 ataaaaaaat atgacgaagc tattgcagca tacgaggaag caaaaaaact gcctaatacc
     8581 taccaggctc cctggtatat gattccgcat attcttttgc ttcaaaaaaa agatgatgag
     8641 gccttaatgg gcttctacaa cggcctttcc aacatatcaa aagacaatca gttttattcc
     8701 agttccttaa tggatatagg catgatacaa aataggctga aaaaaaacta tggtaaagct
     8761 atagaagcct acatgcaaat tcttaaagaa aacggatttg attatgcagc actggaagga
     8821 ctaatgtatg tttcctataa atcaggcaat aaacaaaaag ctgacagtat ttttcttatt
     8881 cttaaaaaag catttgaaga aaagaaactg caggtaaata tgatgaaata caaaagagca
     8941 aaaattgcag aatttgatat agaaggaaaa gaagtatttg tttataaaag ttttattccg
     9001 cctgccaaaa tgattgatat catctatacc ggttatgtga tgagtaatgg aggggaaaaa
     9061 atggaaagaa tattccagac ggaacaaacc attcagctta cggacaactc accaaagcat
     9121 atattatgcg aagtctggac cgatggaaaa ggaagatcnn nnnnnnnnng gggttgggtg
     9181 gaagaacgat gattttactc ccgatgattt tgttgcctta ataaaagaag tactgacagg
     9241 gaaaattaaa gccgcagcaa gcacacgttt aaaataatga gtaattaaca ttcatcaagg
     9301 atccattctt atatatttac atttgcaggg aataattctt gctcaatgca actaacagaa
     9361 gtcacaacac cccaacttgc ccgggatttt atcagggtaa atgttgaatt atataaagac
     9421 gacccaaatt atttcaggcc actggataaa gacatcaatg aagtatttga tgcaagcaaa
     9481 aacaaagctt ttcgttttgg agaagcggta cggtggatat taaaaaatga ccagggaaaa
     9541 ttaattggac ggatcgccgc atttgtcaac aaaaaatata aaaacaaggg tgatgatgtg
     9601 ccggtgggag gtattggttt ctttgactgc atcaatgacc agttggctgc ggatatgctc
     9661 tttgatgtag ccaaacactg gctgatgcaa aaagggatgc aggcaatgga cggccccatc
     9721 aattttggcg aaagggaccg ctggtgggga ttgatcgtag aaggctttca gcccccgctt
     9781 tattgcatga actacaacaa accctactac aaagaactgt ttgaacgcta cggtttcaaa
     9841 aattttttca accaaatatg ttttggcctc gatcctaaaa aagaattaag tcaaaaaata
     9901 ctggacaggc atgcacaact gaaagaaaat cctgccttcc ggtcaagtta tatcaataaa
     9961 aaacagctgg agaaatttgc agctgatttt accatcgttt acaacaaggc atgggccggg
    10021 cacggcggat taaaagaatt gaaaaaagaa caggtactca ccatgtttaa aaaaatgaaa
    10081 cctgtaatnn nnnnnnnnnn nnnnnnnnnn nnnnnntgta taccacaacc aggaccctat
    10141 cgccattttt gtaaacctgc ccgacctgaa tcagtggttt aaatacctga acggcaaatt
    10201 tgacctctgg cataagctaa agtttctctg gataaagaaa acccggaaaa atcatcgctt
    10261 caccggtctt gttttcggca ttgtacctga attccagggc atgggtgtag atgcttttat
    10321 tatcaatgaa tcaaaattcg taatacagcc ccagaccgat tacacggagt acgagatgca
    10381 gtggatcggc gaattcaatc ccaaaatgat caatgtagtg gaaaacttcg gcgatacgtt
    10441 ccggacccgc aaacttgtca cctacaggta tctttttgac cagacaaaag aatttaaacc
    10501 ccacccggtt attctctaaa cttattcaca gaaccaatac tttttcctga agctatgccg
    10561 ggtttcttaa attgcagccg gtatggataa attcattgtt tcggcccgta agtacagacc
    10621 ccagactttt gatacagttg ttgggcaatc acatattact actacgctta aaaatgcaat
    10681 caaacaccag caactggccc atgccttttt attctgcggt ccccggggtg tgggcaagac
    10741 cacctgtgcc aggatattgg ccaaaacgat caattgcgaa aatccgacta aagacggcga
    10801 agcctgtgat aaatgccaaa gctgtaaatc attcagcgaa ggggcttcga tgaactattt
    10861 tgagctggat gctgcttcca acaactcagt agataatatt cgtgacctcg ttgaccaggt
    10921 tcgctttgta ccacaaacag gcaaatacaa agtatatgtg attgacgagg tgcatatgct
    10981 aagttcatca gctttcaatg catttttgaa aacattggag gaacctccct cctatgctat
    11041 ttttatcctc gccactacag aaaagcataa gatacttcca accatcctga gccgatgcca
    11101 gatatttgat ttcaaacgga ttactaccaa cgataccgta gagcatctgc aggaaatttg
    11161 tgaaaaagaa gaaataaaag ccgacaaagc agccctgcag gtaattgcgc aaaagagtga
    11221 aggctgtatg agagatgcgc tgagcatttt ggacaagata gtcagcttta ccaacggaga
    11281 gttgaactat ggtaacaccc tggaacacct gaatatcctg gatgcagaag tttacttcaa
    11341 gctgctggat tgtatggagc agcaggattt atctgccgct ttgctgcttt atgatgagat
    11401 caaccgcaaa ggatttgagg gggatcttgt gctgaacggc tttgccgaat ttatccgaaa
    11461 ccttttggta tgtaaagatg aaaaggcagc cggtctgctg gaagtggtgg aaagtttcaa
    11521 agacaagtat ataacgaccg gtaaaaaaat atcagccgct ttactcatca gtgcactgaa
    11581 tattctcaac gacgcagagt tgaattacaa agccgcnnnn nnnnnnaaca aaaggctgca
    11641 tgtagaactg gcattaatta aactatgcta cctgcagcag gctattgaat taacagcaga
    11701 tggaaatgct gtaagtaaaa aaaaacgggt tgatgccgta aaaccggtag catttcgttc
    11761 tataaatcct tttataccgg ttacagcaga aaataatact ggcgatactg aaaatagtgc
    11821 caaactggta atagaaactc cttctctttc ttcagttcaa gaaccagttc ccggttacag
    11881 gcaaacagga accccgcccg accagcaagg tggaagtaac gagcaaccag gaacaagtaa
    11941 ccagttgacg gccaccggta aaaaactaac agccctcaac aagattcgtc agcaatacca
    12001 gaataatggc aacaatggaa ataccgtcaa ccatccgttg caactggagg aactgcaaaa
    12061 agcctgggcc gattatgtag taaaactgaa agatgctaaa aaccccgcag ctcagccttt
    12121 tgagctggcc gtacttcgta tccgggatga gaatagcttt gaagcgatca ctgccaattc
    12181 tatcgaaaag cagttcattg aacaggacag gaataagtta tttgtatatc tgcgggaacg
    12241 tttacaaaac cggctgctgc aattcagtgt aacggtagaa gagaaagcag agaacaggcc
    12301 tgttatagaa atacccctta gtgcgtcaga acaatacaac aaaatggtag agctatatcc
    12361 gttggttaag gaattaaaag acaagctaaa ccttacactg gactattgac cctttgtatt
    12421 taccggccta tagtttatga agggcagtat tggtcatttt accttaattt cggccaaaat
    12481 tttaaggcat atggccgaag tgatattgat gccccgcctc agcgatacca tgaccgaagg
    12541 agttattgct gcctggcata aaaaagtggg agatacggta aaaaaaggtg acttgttggc
    12601 tgagatcgaa acagataaag ccaccatgga actggaaagc tataaagacg gtaccctttt
    12661 acatgttggt attgacaaag gtggcaaact gcaggtagat gatctgctgg ccatcgtagg
    12721 tgccccgggt gaagacattt ctgctttact gggaagcact aaaacggcga cgaaaaaaga
    12781 agaacctgcg caaactcctt ctccatctgc aaccgcttct accccagcca caacacaagc
    12841 cctcgatatt tcaaaaatgg atgaagtggt gttgatgccg cgattaagcg atacgatgga
    12901 agtgggtgtt attgccgcct ggcataagaa tgtaggagat acggtgaaaa aaggtgaagt
    12961 gttggctgag atcgaaacag ataaagccac catggagtta gagagctaca agaatggaac
    13021 gctattgcac attggtgcgc agaaaggtga aaagatacct gtaaacggtt tgctttgtat
    13081 tattggtgaa aaagggaaag tggatgtaga tgcaattgta gcggccgtaa atgccggcat
    13141 ggtaacaaca aacgctgccg caacacccga tgattcccag cctgctttag ctgaggcatc
    13201 aactacaaca gccgtcacta cccaaccagc cacagcctct tctgaaaatg gaagagtaaa
    13261 agcttctccg cttgctaaaa aattggcggc agaaaaaggt attgatatca gcaccgtttc
    13321 cggaagcgga gatggtggtc gtattataaa agcagatatc gacaaatata aaccttctgc
    13381 aacagcggca cccgctgcaa aagcacctgc gtttacagtt accggccagg aaggttatac
    13441 cgatattccc aactcgcaaa tgcgcaatgt tattgcaaag cggttgggag aaagcaagtt
    13501 cagcgccccg catttttatc tcaccatgga aatcaatatg gataatgcga tggtagcaag
    13561 agctcagttg aatgaactga gtcctgttaa aatcagcttt aatgatatgg tggtaaaggc
    13621 tgtggcgttg gcgcttcgcc agcatccagc cattaattcc tcatggctgg gtgataaaat
    13681 acgtcgctac agccatattc atattggtat tgccgttgcg attgaagatg ggctgatcgt
    13741 tcctgtagtt aggtttgctg atcaaaaaac attgccacag atcgctgctg aaagtaaaga
    13801 actggcaggt aaagcaaaga acaaaaaact acagcctaat gaatttaccg gcaacacatt
    13861 cactatctcc aaccttggta tgatggatat agacgaattc acagcaatca tcaatcctcc
    13921 tgacagctgt ataatggcag ttggaagaat taaagaagta gtggtaagaa agggtgatgg
    13981 ctttggcgta agcaatgtaa tgaaagtaac actcagctgt gatcaccgca gtgtggatgg
    14041 agcatcgggt gcagcattcc tgcaaacatt caagaagtac ctggagaacc cgattaccat
    14101 gttattataa gtaagattac ccgtctataa aacagtaaag ctaccagccc gggtagcttt
    14161 tttttatcaa tcttattctt atggcaggat aattgagaaa taaaccaatg tactgaaaac
    14221 cattaggggt aacgtcttaa aagcgaaaaa tatccattgc ttgtcatgct tttgtgaagt
    14281 tttccctctt acctttcctt caaattcaat cttatggaag acagagacct aaatgggttt
    14341 tcctggcaaa cgctgttttt tgttacaggt atttcttttg tactattcag ccttgtatta
    14401 aaattcctcg acggagctca ttccgttccc atgttatgga taggtgcaat atcgctttcc
    14461 ttaggcttaa taatttgggc tattgatatg gctatcagaa cacctgttcg caagaacgga
    14521 aaatatagtt cctgatttaa ctcccggaaa tattcagcaa taccaatttt ccatccatcg
    14581 ttgataccac caattggctt ttgctcaaaa ctcttactgt atttaccatg gagttatcaa
    14641 ttttatgcgc ccaaacaact ttcttttcag ttgggtcgat acagtagact acgccgcttt
    14701 ttgttccaaa gaatacatga ccatcctttt cgatcagcat ggaaggaaca tgttcataac
    14761 caaaacctgc atgaagtttc catgcagctg actgctgttc ccggctggcg gcataggcaa
    14821 caatggtgtc ctgcatactt tttccataga tccatttacc atctgccgaa ataccaatcg
    14881 actcccgcac acccccatcc ttgcttctcc ataatgtttt accatttaca gcatctatgg
    14941 cagaaaccac cctgtcagga gccattacat agatgatatt gtctgatgca acnnnnnnnn
    15001 nnaatacagg aagccggtga ataattgcgg acagaagaac cattattcca tttccagagc
    15061 aggttaccat tagaacggtt taaggcatac aaatgacggt cccaggcacc aaacacaatt
    15121 ttgtcctggt ataaaagagg agtgctgaca accggacctt ccaacccggc aaacttccag
    15181 atcaactgac catttttact gcttagtgcc aggtaagaat gatcgcttcc tcctataaaa
    15241 acggtatcac cattgatgac cggagaaccc agtacggaag cggcagtctt atatttccat
    15301 atcaatttcc ctttgtttac atcaacacaa taaacatttc cgtcaccaga accaaacacc
    15361 agtttgccgt ttgcaaccgc tggtgatgaa aaaattgacc cttcagttct gaacttccat
    15421 aaaggctttc cgtctttgag tgagaaacaa ctcatcaccc cattctggtt accaacaaac
    15481 actctttcat tggccactgc aggcgtagaa atgatattgg catcagatga aaactcccat
    15541 gcctttttta cctggccata tttatcattg attgaataat caggtcttgg aaactttttt
    15601 tcttgttcgt aatttcttga ttcaatttta acacctgtcc attttctctc atttccggaa
    15661 acaggtcttc tcactgtaaa aagaatggag tcatttctta catctaccag gttgtaaccg
    15721 ccttcattgt ctttagcccg aagattagaa cggcccatta cgccgggaat atcttcaaaa
    15781 ttcattgctt tatttgcatg accatgtccg caaagtatag cccatgtatt gtgtttacgc
    15841 aatctgtctg ttatttcata ccagttatcc aaacccttat caatcgggta atgatttaaa
    15901 aatatcactg gctggttctt cttaactttt ttcagctctt tatccagcca gtttaccgca
    15961 tctcttggca catgcccgtc actcattctt acatnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtgaacatca
    16081 ccccgccgct ttcgctccaa ccggtatcat gattacccgg gataatatac caggggatat
    16141 ccaggctatc taatatttgc ctggccagtt tgatttcttc attggtgccc aactcggtta
    16201 tatcacctgt cagtacgaca aaagcaacat cactcatggc attgatatcc ctgaccgttc
    16261 tgcgaaggtc ttcttcggca gagccgttag gcgatcctat atgggtatcc gaaatgaaag
    16321 caaaccgaaa agattttacc tgcgaaaagg cggtaaggga aaagcccagc aggaaaagga
    16381 gaaaatattt cttcatacgg caagttaata attaggtgca ttttccagta ttttctgtct
    16441 cattaaatga cgaatagttg ccgtagtaag cagcagattt cccggattcc ttaatttcgg
    16501 tactgcaacc ttaactacag attatgatac tattagatat atcgtcctgg tggcaggcgt
    16561 taacctcaat ggagaaaata ttctggggta tctccctgct tttttctgca ctttttttaa
    16621 ttcaattggt tttatcattt atcgccggcg acggtgatag ttcattaggt gatgctgatg
    16681 aatatatcag tggtgatgaa ggaatgggcc atcagttctt taccatcaaa aacctgatag
    16741 cttttttcac catattcggt tggacgggta ttgcatgcct aaaaggaggt atgagtaagg
    16801 gaattgccat agctatagct gtgctggcag gttcattggt agtgatcata atggcatttc
    16861 tatttaaaag tatgagcaaa ctgaaacaaa gcggaacatt acaaataaag aatgctgttg
    16921 ggcttatcgc agaaacatac ctgtttatcc ctcccaaaag gggaggtttt ggcaaagtgc
    16981 atgtgaaggt acagggatca ttacacgaac tacaggccat tacagatgat gaagaacaga
    17041 ttgctacagg taaactggta aaaattactg ctgtgatcaa tgatgctgta cttcttgtaa
    17101 catccaaact ttcttaatca accttaatct tataaatcat ggctgagaat ttattactcg
    17161 ttgtcgcggc ggcgatactt ttctttttca ttcttgtata ttccatgttt cggcgctata
    17221 agcgttgccc gtcagacagg atactggttg tatatggtaa agtgggcaaa ggagcagaag
    17281 gtacacaaac agccaagtgt attcatggtg gtgccgcttt catctggcct ataattcagg
    17341 actattcatt tttagacctc acccccattt ccattgaagt caatctgacc agtgcattga
    17401 gcaagcaaaa tatccgtgtg gatgttcctt cccgctttac tgttggtatt tccacagaac
    17461 cggcggtaat gcaaaacgcc gcagaaagat tgttgggcat gcagcgaccg cagattcatg
    17521 acctggcaaa agacattatc cttggccaga tgcgtttggt ggtagccatg atggatattg
    17581 aagagattaa taacaaccgg gataaatttc tggccaatat tgcttccaat gtagaagcgg
    17641 agttgaacaa gatcggtttg aagatgatca acgtaaacgt taccgatatc aaagatgaaa
    17701 gtggttatat agatgcgctg ggtaaagaag cagcagccaa agccatcaac gaagcaaaga
    17761 tccgggttgc cgagcaagaa agaacaggtg acattggaaa aacaactgcc gaaaaagaaa
    17821 gagacattaa agtagctgag acacagcggg accgtgatat aaatgtagca cttgccctta
    17881 aagagaaaga gatccagatt gccggcgcca accgtgatga gaatattggt aaagcagaag
    17941 cggaaagaga tacaagagtt aaaatggcag aagctaatgc tatagccgta cagggtgaaa
    18001 acctttcaaa aattgaaatt gccaactccg atgccgctag aagggaaaga gaagcagagg
    18061 ctttaaagaa agcgatagct gcagaaaaag tgcaggcagc tagggccctg gaagaagctt
    18121 atctcgccga acaaaaggct gaaaatgccc gtgcagaaag agacnnnnnn nnnnnnnnnn
    18181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa caaaaattga
    18241 tcatcgaagc acaggctgct gctgaacagc taagggaaaa agcaaaaggt gaggcagatg
    18301 ccatctttgc caaactggaa gccgaagcaa gaggtatgta tgaaattctc accaaacaag
    18361 ccgaaggttt ggaacgtatt gtaaaatcag caggcaataa tccaaaggat gccgtgttgc
    18421 tgcttatagc cgacaaatta cctgaactgg tgaaaatgca gaccgaagcc atcaagaata
    18481 tcaaaattga taaagtaacc gtttgggatg gcggcggaca gggtgccgat ggcaagggat
    18541 ccaccgcaaa ttttatgagt ggtctttaca aagctgtacc accgctaaaa gatatttttg
    18601 acatggccgg tatggatctg cccacttatc ttggcaagaa aaaggaagag gcagcagtag
    18661 aggtgaaagc tgaggaagta aaagaggaaa aaaagaagta aagctttttc atgaaatgtt
    18721 ttgaatgaaa aaaatcacca ttcttttaag catttttttt atttatacaa ctgtttttgc
    18781 acaaaaaggt aaatcaggca atatttctta ttacgttttt gacgccgcat ggaaaccttg
    18841 taatactgat gatgccgttt actttgccat tcttgaaaag cttaatgata ctgcatggca
    18901 atggaagaac tatcttttct ccggaccatt gctgagtgtg gaaacctata aagacgaaga
    18961 tggcacaatt ccgcatggtt atttcgcctg gtttgattcg aacggtaata tagattcttc
    19021 aggcgaaact tttggaggaa gaaaacatgg cagctggtat tattttactg actcactttc
    19081 tgtctggcaa accgaaaaat acgagaatgg cattttatct gaacgaaaag acagtatttt
    19141 attacgcaat gaaaaaagaa gtaatgattc cattgggtat ttaccgggtg ataaggaagc
    19201 tgtctttaaa ggtggtacaa atgcctggat taagtatctg caaaataata taaaaatccc
    19261 cggcagggct gagaaaatgg gtaaatccgg aacagttaaa atcttcttta tggtcgacag
    19321 tgatgggtcc ttaaaagatt tacgattata taagtcagtt gaatactcac tggatgaaga
    19381 agctttacgt ctaatccgta agtctccaaa atgggaaccc gcccggcaaa tggataaacc
    19441 tgtcaaagct tatcggattc aacccattac aattgtattc taaatatatt ttcaataaat
    19501 tttgaaaata caatatttac accatagctt tgtgccatga aactcaccga agccaaacaa
    19561 caattcatca gcagctgggg tgccttcggc accaactggg gcatcaaccg cactatggca
    19621 cagatacatg ccttattact ggtgaatccc gatccgctta cccaggatga tatcatggaa
    19681 gaactgaata tcagccgggg caatacgaat atgaatatcc gggaactcat caactggggc
    19741 ctggtagaac gggtattgct taccggggaa agaaaagaat acttcaccgc cgaaaaagat
    19801 atctggaaag tagtaaagca gatcgtaaaa gaaagaaaaa aaagggaact ggaacctatg
    19861 ttacagttac tagacaaact agaagaagtg gaaggcgata aaagagacaa gcatgtaaaa
    19921 acatttgtgg acaccgtaag cagcataaaa aaactggggc ggcaggccga caaaacactt
    19981 gacaccatga tcaaagcaga agaaagttgg ttcatcggtt ccctgatgaa gctctttaaa
    20041 tagccctatt tgataaagcc ggaataccgg cttttaattt tgaattattt ttcaattttt
    20101 tctgaaaata aaaaatatga aactgctaaa acttaccgac ctcgtactgc aactattaat
    20161 aaccattagt ggtattgttt tgggcattat aaaggatgat atacaaatac tattgtacct
    20221 ctactttatc ctgggtggtt ggcaactggc tagttatctg acccattacc tcaccggagt
    20281 aagctgggcc aatagagtgg aaagaagcca atatggccat acagttttat gggtagcggt
    20341 agttggagta ctttgctact tgcttttact tgccgattta ccgttcatag ctttctacct
    20401 gctggcaatg gtaatcattt ctccggtgat ggcctgttgg tattttgtga ttggtcttgc
    20461 tgaatggaga accatacggc acagggaatt aattcatctt aagtaaagaa taatttagtt
    20521 ttacctgatg aaagcaaaaa ccactcttgt tcttggcgca tccgataatc catcccgtta
    20581 cagttacctg gctattaaac gccttcgtaa tcatggccac ccggttttcg ctatcggccg
    20641 gaaacacagc ttcgtggatg atacagagat aatgacagaa aaaaaacaac tggaaggtat
    20701 tgatacagta acactttatt taaatccgca acaccagcag gactactata attatatatt
    20761 atcactgcat ccaaaaagga tcatcttcaa cccgggagca gaaaatgatg agctggcaga
    20821 actggcaaca ttaaaaggga tacaaacaat tgacgcctgc acactggtta tgttaagtac
    20881 aaaccaatac taatagtaga taaacactta gtcacttcga agttttacga ttttggtcgt
    20941 gtactagccc tcgatcttgt ctgaatattt tctttttgct tggattcagc tatttttttc
    21001 acaaatcttt acatcccgtc ccccctgaaa actgcaaccg ccaaatccag gttgccgaac
    21061 aatatcctgt tgacttataa aaaaagttga aatgaaaaac cgttcattca tcgcactatg
    21121 cgttagtgcc ctcattttta atgtacaact cctcgcacaa aaccaggatg taccattaaa
    21181 tgaacctgat tacaacaagc ctttgttgtt cagaagtttt cccgacagca ttccggtaaa
    21241 cattgccagt tttgacagag ctgtttcttt ttcccagggt gaagacgtat cagtgaatat
    21301 ctctgataat gttcattttc ccttcccggg aaaagtaatg tcaatcgcca gcaaatatgg
    21361 caacagcatc cagagtgttg ttattaaatc caatgtatat gatggcgctg gtcttaccat
    21421 ttcgaaagta acagacagca acggtaatat tttctataca ggccgcattg taagtatgaa
    21481 aagcggtgat ttgtttgagc tggtaaagac cgagagcggc tatttcctgg taaaaagaaa
    21541 attttatgac cttataaatg aataactgac ccgtttttga tcccgtatcc ctactagtac
    21601 ctattgaaaa acattttaaa atccgccttt gttatgaaaa actatttaca atcctgcctt
    21661 atggcgctgt tcctgttcgg tgcagtgaag tccaatgcac aggtacctgt atatagcagt
    21721 ttgccgtcag caacagccgt cgtcttcctt gattttgacg ggcatacagt aaacggtacc
    21781 agttggaact atgcaggtcc gatctattgc ggtgcttcag gtatgaacag tacccagatc
    21841 accgaagtat ttaaccgggt agctgaggat tacaggccct ttaatgtgaa tgtaaccaca
    21901 gattctacta aatacctcgc tgctcctgcc aaccggcgta tgagggtgat cctcactgtc
    21961 agttacgaat ggtatggcgc tgctggtgga gtagcttttg taaactcctt tacttggggt
    22021 gatgatactc cttgttttgt gttcacttca ttgctgaact acaatacaaa gaatatctcc
    22081 gaagcttctt cgcatgaagc gggacataca ttaggcctgt atcaccaggc ccgttatgat
    22141 gccaactgtg tgaagcaatc tgattatcac tccggtgccg gtgctggtga aattggctgg
    22201 gctcctatca tgggagtagg ctattaccaa aaccttaccc tttggaacag tggccccaat
    22261 ccttatggct gtaataatat tcagaacgac cttactgtta tcacaactaa taatggtttt
    22321 agcttcagaa cggatgatca tactgcatca ttcccgacag caaccaacgc tacgtttgta
    22381 aataaccagt ttaccgtaag tggtgtaatc acacaaaaca ccgaccagga tctttttagg
    22441 tttacaaaac caacagcagg caggttccag ctcagcgcca ttccttataa tgtaggtacc
    22501 ggcaactccg gttctaacct tgacctgcag attaccttat ttgatggtgc acaaaaccag
    22561 atcagtgttt ataatcccgg tactttgctc agctctgtaa ttgatacttt actgaatgcc
    22621 ggtacatatt acctgagagt ggaaggaaaa ggaaacatgt atgcgcctaa ctatgccagc
    22681 cttggctcct attcactgca gggcagcctt actgcaggcg gtacactgcc tttaagaaga
    22741 ctggaactta ccggtgaact gaatggtgac aaacaccggc tgaactggct gatagatgct
    22801 gatgagcagg tggtagaaca agtactggaa gtggctacgg atggccgcaa cttcagcccg
    22861 gtagttaatc cgggaaatag tgaccgcaca tttatgtaca ggccttatgt aagcacctct
    22921 gcgcaatacc gggtatttgt aacatttgat aatggcaaat cctattattc caatgtggtt
    22981 acattgaaag gcaccggcac tgtggagaaa ccaagactgg tgaccaacct ggtcagtaat
    23041 aacctgatca ccgtaacaag cccgggtgtg tttggctata cagtggtgga tatgaatggc
    23101 aaggtaaccg ccaggggaca actgacaaac ggcctgaata atatcccggc ctctttcatg
    23161 aacggtggaa tgtacatcat ccgctttgtg aatggcaacg agcaatggac tgacaaattc
    23221 gttaaacaat aaacctttcc ccccttctgt ttacagaagg ttaatccgtg cccttttgta
    23281 gttaagaggt tttcctgtaa cttaggaaaa cctcttttca tatgcgttgg agaaaattta
    23341 atggcgaccc tattgatctg cccatcaagg atgctgttga aaatgccata aaacgggaaa
    23401 cagaaaaagg attccgtttg aaagtatgca tcggcaccga ctcacaggta aaaggccagg
    23461 aaacagagtt tgccacagtc atcgtttttc tgagagaagg acatggcggt ttcatgttca
    23521 ttcacaatga aaaaacccgc cagcagttta ctatcaaaga acgaatgctg atggaagtgg
    23581 ctaagagcat tgaaattgct tatgagctct gcaacctgtt taccgtgtat gatgtagata
    23641 tggaagtgca tgccgacatc aacaccaatc ccaacttcaa gagcaatgat gccctgaagg
    23701 aagccatggg ttatatcctg ggtatgggtt ttgccttcaa agcaaaacca gaagcctttg
    23761 ccagcagcag ctgtgcaaac aaaattgtga actgattttt aacggctgca ggccgtacat
    23821 gcgggaagaa attcctgcac atagctttgt cacccctgct ttttccttct cttcatcaat
    23881 cccattacca ataacgccag gaatgctgcg ccaatcgcca gtcttgtgat ggctgcgctg
    23941 cgctggcggt ctttattttc tttttgaatc tccagtaact ggtcaacgtt attctgaata
    24001 ttgatggcta cctgctgcct gtctatcttt ttggcaatgc tgtccttaaa tcgctgaatt
    24061 ttttgtgcta ctgaatcttt attcagcatt tgttgcttta tatctccctt acggatttta
    24121 tcagtagcct gggcatccac aaacaatggc aaaatgagca gcaatgaaat caaaaatcgt
    24181 ttcatccaat aaatttaaag gagcaaaagt gaactatttt acagaaggca aaccggcagc
    24241 tgggataacc gggtcttgtt gtacaaggaa acgggcattt tgggcctgaa caccgctgta
    24301 cattttctca attttcttac tgtcttcctg tgcgagggag aaagtaacga gtaccataat
    24361 aaatagtact acaacaatat ttcgttcgga taagatggtt ttcatgaaaa gtccagtagt
    24421 tgggtgcaaa ttaggtgaaa atattcaaca aaaaatacca caaatggggt aaaacaatct
    24481 aagcctttga aaccccggca ttaaagtccc ggatataatt caataaaaga tccctgccct
    24541 ccctagttag cgccgatgtt acaaaatatg gcgggtattc ttcccagtta tccgctaatg
    24601 ccgttagaaa agccgccaca tttcgtttgg tagccccggg cttgttttta tccgccttgg
    24661 taaaaaccag tacaaaaggc acctgccact cccccaactg gttgatgaat tcaatatcta
    24721 ttttctgcgg ctcatgccgg ctgtcgatca gcacaaacaa acaaacgagg ttttcccgtt
    24781 tccggatgta cgactcaatc atattccccc agttgttcct tgatttctgc gacactttcg
    24841 cataaccata accgggcagg tctaccaggt accatttttt tttgtcggaa gaaaccactt
    24901 caaaatggtt gatcagttgt gtttttcccg gtgtggcaga tatcttggcc agtcccttct
    24961 tattagtcag catattgatc aatgaagact tgcccacatt actgcggccg ataaaggcat
    25021 actcctgctt atccgccggc gggcactgct tccagccggg gctgctaatt atatatgtag
    25081 ctgagataat ttccatttgt tgagcatcaa acaagaactg cgaagtaaaa ccttaaactt
    25141 taaacctcaa accttaa
//
LOCUS       DAORLS010000010        24909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1099_length_24909_cov_1.894061, whole genome shotgun sequence.
ACCESSION   DAORLS010000010 DAORLS010000000
VERSION     DAORLS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24909
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_24909_cov_1.894061"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK977_01710"
     CDS             complement(<1..263)
                     /locus_tag="PK977_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HRF16846.1"
                     /translation="MSFDNTEFAFQYKSDKELKKAHFLFSMMSRPWLVKIGAKMAPWA
                     LKVGLPVKGIIRKTIFSQFVGGETLEQIDPVAQMLAKYNVQIIL"
     assembly_gap    272..368
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(404..>690)
                     /locus_tag="PK977_01715"
     CDS             complement(404..>690)
                     /locus_tag="PK977_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF16847.1"
                     /translation="TPMAGAAAYSASKYGLTGMMQSIREEVKRTEIRITNIFMGGVDS
                     SFWDNIDLRVQREKMIRTEEAARAIWYLCQQPASGVVSEMVLQPFNHQAI"
     assembly_gap    691..756
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<757..1124)
                     /locus_tag="PK977_01720"
     CDS             complement(<757..1124)
                     /locus_tag="PK977_01720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF16848.1"
                     /translation="MESVKGKNILIAGATGGIGSAVAKLLAASGANLFLTGRSSDKLS
                     VIATQSGISSDRTFSADISNPDQVSALSKKYFSIYPSIDILINTAGIGIIKPIENLSE
                     DEFLQTIHYNLYGSFLLVKA"
     gene            1138..>2474
                     /locus_tag="PK977_01725"
     CDS             1138..>2474
                     /locus_tag="PK977_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRF16849.1"
                     /translation="MQQQTCRSFVEAIMKSVSLVFPHQLFAVNPALKKDREICLIEEF
                     LFFRQYNFHRKKLVLHRASLKAYEAYLLSKNYKVNYIETTTNIADIRLLINKLANRSV
                     TEIHYTDTVDNWLEKRIIYAAKAHQIRLVKYPTPNFLNQPGDWDHFFSTKKTYFQTDF
                     YIEQRKKRRLLLEKDMKPVGGKWTYDAENRLKFPKHETVPLIKRPEKNKYVDEAIKYV
                     SKYFPGNYGSMEDFSYPVTFTDTDKWLDDFLENRFSKFGIYEDAMVQNESILYHSVLT
                     PMLNIGLINPQQIIDKAIQAAIDKEIPLNSLEGFIRQIIGWREFIRIVYEREGTRQRT
                     TNYWKFTRKIPLGFWNGITGIHPIDTVIKRLLKTGYCHHIERLMVLGNFMLLCEFDPD
                     EVYRWFMEMFVDSYDWVMVPNVYGMTQFADGGLMTTKPYISGSNYLLKMGDWH"
     assembly_gap    2475..2613
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2791..3048)
                     /locus_tag="PK977_01730"
     CDS             complement(2791..3048)
                     /locus_tag="PK977_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HRF16850.1"
                     /translation="MDIDRLVEMAWEDRTPFEAIWVQFGLGEKDVIALMRRHMKRSSF
                     TMWRKRMAGRSTKHQKLRITGVSRFKCSRQRAISGNKISKR"
     assembly_gap    3116..3139
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3164..>3925
                     /locus_tag="PK977_01735"
     CDS             3164..>3925
                     /locus_tag="PK977_01735"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HRF16851.1"
                     /translation="MQHIEFPTSYSEILKRIDEIDPIEYARSRNYLNGSVTYLSPYIS
                     RGVISTKKILQSILEKGFTPAQSLKFIQELAWREYFQRVWQHLEDDILDDIRRTRTGV
                     NNRQISAALINAATGIESIDKGINELYETGYMHNHLRMYVASIACNVAKSYWDMPSRW
                     MYYHLLDGDIASNTCSWQWVAGSFSSKQYFANQENINKYTGSKQINTYLDKAYDELAE
                     QNIPEPLNKLISPVLSTNLPEKNTPVLNEHLPTLLY"
     assembly_gap    3926..3935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3936..4309
                     /locus_tag="PK977_01740"
     CDS             <3936..4309
                     /locus_tag="PK977_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16852.1"
                     /translation="SYNLDPEWRTDMEANRILLLEPSHFRQFPVSEKVLAFIIQLASN
                     IKGIQLFVGELNEIPGLNNSKAIYSKEHPAFKHFPGIKVERDWLFPEIKGFYNSFFSY
                     WKQCERSLSKMEKRVPALLRA"
     gene            4351..4866
                     /locus_tag="PK977_01745"
     CDS             4351..4866
                     /locus_tag="PK977_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRF16853.1"
                     /translation="MEPRKASDSLTIMTELVLPNDTNIFGNLMGGRLMYWMDIAAALA
                     TQKHCNAPAVTASVDNISFESPIKLGNVVHIEAKITRAFNSSMEVHLKVWGEDLIHQY
                     KYKSNEAYYTFVALDPNRKPRPVPPVIAETEEEKKLYDGALRRRQLRLILGGKLKPDD
                     ATELKALFVKD"
     gene            complement(4867..5643)
                     /locus_tag="PK977_01750"
     CDS             complement(4867..5643)
                     /locus_tag="PK977_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRF16854.1"
                     /translation="MSFISSISDRLRHFHPVRKIVYLIVGAFSYPGLTMVNRLKITGT
                     EHLKDLPRRRVLFVSNHQTYFADVITFLHIFCAVKWGKMNRLGVPYYLLNPFTRVNYV
                     AAEETMKGSFISRLFLLAGGLTVKRTWREGGKEVRRGLDPSDTRKITRALENNWVITF
                     PQGTTKPFAPGRKGTALIIKQIKPVVIPVVVSGFWRAFNKKGLKFKKKGSLLSVTFKE
                     PLQINYEDSAENILAQVMDAIEQSKKHMMMGKHHWLTTDK"
     gene            complement(5773..>7377)
                     /gene="rpsA"
                     /locus_tag="PK977_01755"
     CDS             complement(5773..>7377)
                     /gene="rpsA"
                     /locus_tag="PK977_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRF16855.1"
                     /translation="ISLNEFRDIPNLSVGDEVEVMIVEKEDREGHLNLSRRQARITRA
                     WEKIVEVHKTGEVITGTVTSKTKGGLIVDVFGMETFLPGSQIDVKPVTDYDQFVGKTM
                     EFKVVKINETIKNAVVSHKALIESDIEAQRAEIMSKLEKGQVLEGTVKNITDFGAFMD
                     LGGLDGLLYITDISWGRISHPSEVLKLDQKINVVVLDFDDEKKRISLGLKQLTPHPWD
                     VLADNIAEGNIVKGKVVNIEDYGAFLEIQPGVEGLVHVSEITWANTPINAKEFFKLGD
                     EYEAKVVTLDKASRKMSLSIKQLSADPWNDIETKFAEGSRHTGLVKNITNYGVFVELA
                     PGIGGMIHISDLSWLKRFNHPSEYTKVGSNIDVVILGIDKENRKLQLGHKQLEEDPWN
                     TLQDTFAIGSIHEGTVSRRDDKGAIVQLPYGLEGFAPNRHLSKEDGKSIAADETAQFM
                     VIEFDRNEKRIVVSHTRIWEQGQVEAAEAAKKEARAEAETTRKAVKSVQNKVEKATLG
                     DLGVLADLKKKMDSGEAASSEEAKPE"
     assembly_gap    7378..7493
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7494..7689)
                     /locus_tag="PK977_01760"
     CDS             complement(<7494..7689)
                     /locus_tag="PK977_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16856.1"
                     /translation="MFDNIIKKQLNADAQESASAEQAEVTTATTTAPVEAVATAHDDF
                     DWSVDRRNVISYSAEEREKYH"
     gene            7904..8851
                     /locus_tag="PK977_01765"
     CDS             7904..8851
                     /locus_tag="PK977_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF16857.1"
                     /translation="MKILSRLALPIWCRRLTTNKPEILKEKGPLLLASNHPNSFLDSV
                     LLDTVFQQPVWSLARGDVFKKPFYIKLLTALKILPVYRTSEGVENLSENYKTFEECIT
                     IFRKGGIITIYSEGKCINEWHLRPLKKGTARLAVKAWDENIPLKIVPVGINYSSFSRF
                     GKNIFLNFGEIITATDIINGGADGQRHLAFNNKLKSEQEKLVFEIPAGNKELRQEKLA
                     VKISLEKRILLSLPALLGWLLHLPLFLPVKQFSRQKTKGTDHYDSVITGILLIIYPFY
                     LILISVFLFLITLSWWYLLVLTVFPFTAWAFVQLKPQLD"
     gene            complement(8853..10079)
                     /locus_tag="PK977_01770"
     CDS             complement(8853..10079)
                     /locus_tag="PK977_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF16858.1"
                     /translation="MAEKKLIVIGGGAAGFFCAVNAARLNPLLKVIVIEKSNKLLSKV
                     KVSGGGRCNVTHACFDIGEMSRNYPRGANFVKKTFHHFFTTDTIKWFDERGVTLKTEE
                     DGRMFPVSNTSETIIRCILKEASTYNVAIMLNAEVKKLEKTQEKFHVELSDHRQLNAD
                     YVCVASGGYPKSSMFDWLKETGHTIEEPVPSLFTFNLPGHPIRELMGISAENVKVKIQ
                     QSKLAESGAVLITHWGLSGPVVLKLSAWGARELAKLNYEFTISINWLPELNEQTLKER
                     FLQIRNENASQRIKGRNMHGLPGRLWEFLAMQSGVTETMRWAEMPSVVQNKFIKHLCN
                     YELQVTGKTTFKEEFVTAGGIKLYEIDHNTMMSKKVSNLYFAGEIIDVDGVTGGFNFQ
                     HAWTSGFIAAKSIAAG"
     gene            complement(10098..>10350)
                     /locus_tag="PK977_01775"
     CDS             complement(10098..>10350)
                     /locus_tag="PK977_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16859.1"
                     /translation="KHTILPGMCFTIEPMINMGKKDVYTESDGWTVRTMDGKPSVHFE
                     HDVCVRKSKADILSDYSPIEKAEAANANLFTAKIFQPLS"
     assembly_gap    10351..10422
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10423..>10847)
                     /locus_tag="PK977_01780"
     CDS             complement(<10423..>10847)
                     /locus_tag="PK977_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRF16860.1"
                     /translation="SKTLTEVAKHLKPGITTLSIDRMIGEYIKDHHAVPSFLNYHGYP
                     YNSCISVNDVVVHGFPGDNELREGDIVSVDVGVILNGWHGDHAYTFAIGDPGPEVMQL
                     ILVTKQSLYKGIEKAIAGNRVGDIAFAIQEHTEKKHGYGV"
     assembly_gap    10848..10869
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10996..12330)
                     /gene="secY"
                     /locus_tag="PK977_01785"
     CDS             complement(10996..12330)
                     /gene="secY"
                     /locus_tag="PK977_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRF16861.1"
                     /translation="MKKLIQTLKNIWSIEELRGKILFTLMMIAIYRVGTHIVLPGIDP
                     NALESGQNSTGILGLIDAFSGGAFNQASIFALGIMPYISASIFMQLMTVLVPRFQKMQ
                     KEGESGRKKINQYTRYLTVAVTILQASAYVTYLRGMTQAGGIMPAYANFFWLSTVVVL
                     TAGTLFVMWMGEKITDKGLGNGTSLIIMIGILARLPQSFLQELSAKSVRNGEILVFIV
                     EIAILIAIIMGCILLIQGVRKVPVNYAKQIVGNRQFGGARQFLPLKVNSAGVMPIIFA
                     QAIIFIPTIFANFNPTGGGSFLRALNDHSNVWYMLIYSIVVIGFTFLYTALIFNPKQI
                     ADNLKQNNGFIPGVKPGQPTADYIGAIMDKITLPGAILLAFVGILPGIAQRFGVTQQF
                     STFFGGTSLLIMVGVILDTLQQIETQLLMRQYDGLMKSGRIQGRQTTSAVQM"
     gene            complement(12406..>12691)
                     /locus_tag="PK977_01790"
     CDS             complement(12406..>12691)
                     /locus_tag="PK977_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HRF16862.1"
                     /translation="PIQRRIPKRGFKNPHRVEYKVFNLGQIEQLAEKYNFTEVSLENL
                     YINGLVSQNDRVKILGNGEIKGKFTFKVNAVSDKAKSAIEAAGGSVEIVK"
     assembly_gap    12692..12955
                     /estimated_length=264
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12956..13125)
                     /gene="rpmD"
                     /locus_tag="PK977_01795"
     CDS             complement(<12956..13125)
                     /gene="rpmD"
                     /locus_tag="PK977_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRF16863.1"
                     /translation="MKKIKITLVKSPIDRPERQKLTVQALGLNKTNSSKEVEATPQIM
                     GMLRKVTHLVKV"
     assembly_gap    13232..13374
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13375..13678)
                     /locus_tag="PK977_01800"
     CDS             complement(<13375..13678)
                     /locus_tag="PK977_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRF16864.1"
                     /translation="MSKLNVNRVKAGDLELKDKVVAINRVVKTTKGGRAFSFSALVVV
                     GNENGVVGHGLGKAKEVQEAITKGIEDAKKNLIKVPVMKGTIPHDQWAKEGAAKVLI"
     gene            complement(13712..14071)
                     /gene="rplR"
                     /locus_tag="PK977_01805"
     CDS             complement(13712..14071)
                     /gene="rplR"
                     /locus_tag="PK977_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRF16865.1"
                     /translation="MNPKVKSQRRQNIRYRIRRKISGTTVKPRLSVYRSNSDIYAQLI
                     DDTKGQTLASASSRDKDIAAQKVTKSEKSKLVGAAIARKASELGIKDVTFDRGGYLYH
                     GRVKSVADGAREGGLEF"
     gene            complement(14123..14677)
                     /gene="rplF"
                     /locus_tag="PK977_01810"
     CDS             complement(14123..14677)
                     /gene="rplF"
                     /locus_tag="PK977_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRF16866.1"
                     /translation="MSRIGKKPVVAPNGVTISVGKDNVVTVKGPKGELKQAIDRDIAV
                     EVKDGQITFARPTDQIRHRAMHGLYRSLISNMVKGVTEGFERKLELIGVGFKAANQGN
                     VLDLALGYSHNIIFEVPKELKVATEQLKGQNPIITLEGIDKQLIGQVAAKLRSLRKPE
                     PYKGKGVRYVGEVVRKKAGKAAGK"
     gene            complement(14783..>15022)
                     /gene="rpsH"
                     /locus_tag="PK977_01815"
     CDS             complement(14783..>15022)
                     /gene="rpsH"
                     /locus_tag="PK977_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRF16867.1"
                     /translation="SKQGIIKIALKYDPATKQPAIQSMERISRPGLRQYSKPADFRRV
                     KSGLGIAIISTSKGVMTDKEAKAQNVGGEVLCYVY"
     assembly_gap    15023..15219
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15258..15527)
                     /gene="rpsN"
                     /locus_tag="PK977_01820"
     CDS             complement(15258..15527)
                     /gene="rpsN"
                     /locus_tag="PK977_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRF16868.1"
                     /translation="MAKTSVKARQRKRERMVAQHAAKREELKKAGDWKALDEMPKNSS
                     KVRLKNRCQLTGRPKGYVRYFGISRVALRDMALNGKIPGLKKASW"
     gene            complement(15543..16118)
                     /gene="rplE"
                     /locus_tag="PK977_01825"
     CDS             complement(15543..16118)
                     /gene="rplE"
                     /locus_tag="PK977_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRF16869.1"
                     /translation="MSTKTYTPRLADKYKKDVVPALVKKFGYATVMQAPKLEKICLNR
                     GVNGAVSDKKLVDIAVEELTTITGQKAVTTMSKKDISNFKLRKNMPIGARVTLRGDKM
                     FEFLDRLISVALPRVRDFKGVNEKAFDGRGNYTLGVTEQIIFPEIDIDKVNKITGMDI
                     TFVTTANTDEEAYELLKELGMPFKNAKNNSN"
     gene            complement(16118..>16368)
                     /gene="rplX"
                     /locus_tag="PK977_01830"
     CDS             complement(16118..>16368)
                     /gene="rplX"
                     /locus_tag="PK977_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRF16870.1"
                     /translation="VKQVLVDEAKVIVEGVNIITKHTKPSAQNTKGGIVKKEAPIHIS
                     NVMLWDPAQKAGARVTRVRKDGKTERVFKTKKTQGGKK"
     assembly_gap    16369..16394
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16516..16884)
                     /gene="rplN"
                     /locus_tag="PK977_01835"
     CDS             complement(16516..16884)
                     /gene="rplN"
                     /locus_tag="PK977_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRF16871.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGNSGQRYARIGDKIVVTVK
                     DALPQGGIKKGTVAKAVIVRTTNKLRRKDGSYIRFDDNAVVILNQADEPRGTRIFGPV
                     ARELRDKGYMKIISLAPEVL"
     gene            complement(16961..17218)
                     /gene="rpsQ"
                     /locus_tag="PK977_01840"
     CDS             complement(16961..17218)
                     /gene="rpsQ"
                     /locus_tag="PK977_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRF16872.1"
                     /translation="MEARNLRKTRIGVVTSNKMTKTITVAVERKVKHPIYGKFVKKTT
                     KFHAHDEKNECSIGDVVKIMETRPLSKTKRWRLVEVVEKVK"
     gene            complement(17232..17441)
                     /gene="rpmC"
                     /locus_tag="PK977_01845"
     CDS             complement(17232..17441)
                     /gene="rpmC"
                     /locus_tag="PK977_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRF16873.1"
                     /translation="MSKKADFVKSIHGMNEQDLKARLEEDKQRLKKLEFAHAISPLEN
                     PMTIRGLRRDIARLKTELKLKQQQA"
     gene            complement(17462..17884)
                     /gene="rplP"
                     /locus_tag="PK977_01850"
     CDS             complement(17462..17884)
                     /gene="rplP"
                     /locus_tag="PK977_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRF16874.1"
                     /translation="MLQPKRTKHRKMQKGRMKGDAKRGTTISFGSYALKALDSHWITD
                     RQIEAARQALTREMKREGNVWIRIFPDKPITRKPLEVRMGKGKGNLEFWAAVVKPGRI
                     LFEVDGVSEQVARASLALAAGKLPIKTKFIVRRDLVTA"
     gene            complement(17922..18695)
                     /gene="rpsC"
                     /locus_tag="PK977_01855"
     CDS             complement(17922..18695)
                     /gene="rpsC"
                     /locus_tag="PK977_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRF16875.1"
                     /translation="MGQKANPIGNRLGIIRGWESNWYGSKKDYAGKLIEDNKIRTYLN
                     ARINKGGISKIVIERTLGKLIVTIHTSKPGIIIGKGGGEVDRIKEELKKLTGKEDVQI
                     NILEIRRPELDAMIVADTIARQIENRINYKRAIKMSIASALRMGAEGIKVKVSGRLGG
                     AEIARSEEIKQGRVPLHTFRMDIDYANVFALTVYGKIGIKVWICKGEVLTKRDLNPNF
                     VGGKSDVSDRRERRDEGGGGGRRDDRRGDRRGGGRERRS"
     gene            complement(18717..19064)
                     /gene="rplV"
                     /locus_tag="PK977_01860"
     CDS             complement(18717..19064)
                     /gene="rplV"
                     /locus_tag="PK977_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRF16876.1"
                     /translation="MEAVAKLRNYPTSPRKMRLLADLIRGQKVEKVLAELEHNPKHPA
                     VPLRKLVLSAISNWKQKNEGGDESQLVIKTIFVDGARTLKRMRPAPQGRGYRVRKRSN
                     HVTVIVDTKENKN"
     gene            complement(19082..19351)
                     /gene="rpsS"
                     /locus_tag="PK977_01865"
     CDS             complement(19082..19351)
                     /gene="rpsS"
                     /locus_tag="PK977_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRF16877.1"
                     /translation="MARSIKKGPYVASHLEKKVLAINEGKNKKGVIKTWSRRSTITPE
                     FVGHTFAVHNGNKFIPVYVTEFMVGHKLGEFAPTRQFKGHSKKEG"
     gene            complement(19384..>19933)
                     /gene="rplB"
                     /locus_tag="PK977_01870"
     CDS             complement(19384..>19933)
                     /gene="rplB"
                     /locus_tag="PK977_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRF16878.1"
                     /translation="DGEKRYILAPQGLQVGAKVESGPDVAPEVGNALPMKNMPLGTNI
                     HNIEMQPGQGGKLARSAGSSAQLTNKEEKYAVLKMPSGELRKVLINCYATVGVVSNSD
                     HSLQSMGKAGRNRWKGIKPRNRGVAMNPVDHPMGGGEGKASGGQPRSRNGQYSRGLKT
                     RTKGKGSDKLIIQRKNGSKLAK"
     assembly_gap    19934..20056
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20344..20468
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20469..20585)
                     /gene="rplW"
                     /locus_tag="PK977_01875"
     CDS             complement(<20469..20585)
                     /gene="rplW"
                     /locus_tag="PK977_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRF16879.1"
                     /translation="MKLSEVLIKPILTEKANSQQDKLRRYAFKVAKKANKLEI"
     gene            complement(20652..21287)
                     /gene="rplD"
                     /locus_tag="PK977_01880"
     CDS             complement(20652..21287)
                     /gene="rplD"
                     /locus_tag="PK977_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRF16880.1"
                     /translation="MQIEVLNSKGKATGRTVELPEEIFGAEPNNHVIYLAVKQYLAAQ
                     RQGTHKVKTRAEVKGASRKLHRQKGTGGSRKGNIRNPLYKGGGTIFGPKPHSYDIKLN
                     RKVKDLAKISALSYKAKDNAIMVVEDINMDTPKTKTFVDILGNLKVADKKVMFVQPEY
                     NDNVELSLRNVPSVLGVLLSDINTYDIVNSEVLVLTESAAKIFADDEKAEA"
     gene            complement(21337..21954)
                     /gene="rplC"
                     /locus_tag="PK977_01885"
     CDS             complement(21337..21954)
                     /gene="rplC"
                     /locus_tag="PK977_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRF16881.1"
                     /translation="MKGIIGKKIGMTSIFDPEGKQTACTIIEAGPCVVTQVKTKESDG
                     YNALQLSFGDKKEKHSTKAEKNHFAKAQTAPKKFSKEFRDFAIEKNIGETVTVDIFAE
                     GDKVEVVGTTKGKGFQGVVKRHGFHGVGQQSHGQHDRQRAPGSLGNSSDASRVMKGMR
                     MAGRMGNDRVKLKGLKVVKIFADKNYILVSGSVPGHNGSIVLIQK"
     assembly_gap    21956..21987
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22221..22526)
                     /gene="rpsJ"
                     /locus_tag="PK977_01890"
     CDS             complement(22221..22526)
                     /gene="rpsJ"
                     /locus_tag="PK977_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRF16882.1"
                     /translation="MSQRIRIKLQSYDHNLVDKSAEKIVKTVRSTGAVVTGPIPLPTR
                     RKIFTVLRSPHVNKKSREQFQLATHKRLLDIYTSSSRTVDALSKLDLPSGVEVEIKA"
     assembly_gap    22935..23096
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23097..23730
                     /locus_tag="PK977_01895"
     CDS             <23097..23730
                     /locus_tag="PK977_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16883.1"
                     /translation="EANYAFDKINETSSTIHQYLGQVKEILEAIITMAPYIVMELEKG
                     NSSVKELVIKAATIRDQYSQAFTLSLKRKIGNEMQQLFFELTAASLKMMNREDMIVND
                     LLWTNYSDSEIQELEKRMTAPSFKIEKEVAAVEPKVATEVSLSSVNITPLISLSDILP
                     VSEKLSMLIRRVLAPGKWRFANRVYNIIRPHHLFGIMPAIICTTMFELSA"
     gene            complement(23696..>24909)
                     /locus_tag="PK977_01900"
     CDS             complement(23696..>24909)
                     /locus_tag="PK977_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HRF16884.1"
                     /translation="YSTIIKDNKVRNNRIGIAIEHGQDNQIWYNNFSKDREAIRLWAR
                     KEQPADWIYALKRDTRSREYTIAGNSFNNVANGLNISRTSAVKIFGNTYSGSAAYLKI
                     DSTVTEIDSSERPLVDFAGWYIDTLNVKPGNPFKESNKYSGRKNIMVNEWGPYDFGYP
                     VIWNTNPTDSSPMMKFDLIAPAGSWKIIRFWGLHNISAMNGTFPAVLTAEKIKGERTD
                     IFIELGYNGMSYTNQFGKRIAAGKPSRFYFEKFFQPVDWELLFFSLDTNSHNPIKEGS
                     LFSIYEKKAPFKTEKTNRLDYTWWGGIREGSFQHKQFITSANGSFTVPKGTYELSVTW
                     DDAVRIYLDEKLVLEEWNPSKYKFDESPNRKIRLDLEGFHRLRVEHLELGGFATLSVK
                     IRPIIQTLWYK"
BASE COUNT         6324 a         5298 c         4428 g         7241 t
ORIGIN      
        1 agtatgatct gtacattata ctttgccagc atttgggcta ccgggtcaat ttgctccaga
       61 gtttcaccac caacaaactg agaaaagatt gtttttctga taataccctt aaccggcaag
      121 cccactttta atgcccatgg agccatttta gccccgattt taaccaacca cggtctggac
      181 atcattgaga aaaggaaatg tgcctttttt aattctttgt ctgatttgta ttgaaatgcg
      241 aattctgtat tgtcaaatga aatgacattc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnat agctgaaaga aggctcaaaa aaagagagaa taatcatatt gcctggtgat
      421 taaacggctg caataccatt tcactaacta ccccgcttgc aggttgctgg cataaatacc
      481 aaattgccct ggcagcttct tccgttctga tcattttttc ccgctgtacc cgcagatcaa
      541 tattgtccca aaaagatgaa tcaaccccgc ccataaaaat attagtaata cggatttctg
      601 tccgcttcac ctcttcccgg atgctttgca tcatgcctgt aagtccatat ttgctggcac
      661 tgtaggcggc tgcgccggcc attggtgttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaag cctttacgag taaaaaactt
      781 ccgtataaat tgtagtgaat ggtttgcaga aactcatctt cgctaaggtt ttcaataggt
      841 ttgataatac caatacctgc tgtatttatc agaatatcga tggatggata aatggaaaaa
      901 tattttttgc ttaacgctga cacctgatcc ggattggata tatctgcaga aaaggttcta
      961 tcggagctga tgccggactg cgtagcgatg acagagagtt tatcactgct tcttcccgtt
     1021 aaaaagagat tggcaccgct tgctgctaat agcttagcca cagcggaacc gattccgccg
     1081 gtagctcctg caatgagaat gtttttacct tttactgatt ccatacctta ttcttttgtg
     1141 caacaacaaa cctgccgctc atttgttgaa gcaatcatga aatcagtttc ccttgttttt
     1201 ccgcaccaat tgtttgctgt aaacccagca ctaaaaaaag acagggagat ctgcttaatc
     1261 gaagaattcc tttttttccg gcagtacaat tttcaccgga aaaaactggt actgcacagg
     1321 gcatccctaa aagcctatga agcttacctg ctttctaaaa attacaaagt caattatatt
     1381 gaaacgacca ctaacatagc tgacatacgt ttactcatca ataaattggc caaccgatcg
     1441 gtaaccgaaa tccattacac agataccgtg gacaactggc ttgagaaaag gattatttac
     1501 gctgccaaag cacatcagat caggctggta aaatacccca cacccaattt cctgaaccaa
     1561 cccggggatt gggatcattt cttttccacc aaaaaaacat atttccagac cgatttctat
     1621 atagaacagc gaaagaaaag aagattactg ctcgaaaaag acatgaaacc tgtcggtggc
     1681 aaatggacat acgatgctga gaacaggctc aaatttccca aacatgaaac cgtgcctttg
     1741 atcaaaagac ccgaaaagaa taaatatgta gatgaggcca taaaatatgt ttcgaaatat
     1801 tttccgggca attatggcag catggaagac tttagctatc ccgttacttt cactgataca
     1861 gacaaatggc ttgacgactt cctggaaaac cggtttagca aattcggtat ttatgaggat
     1921 gcaatggtac agaacgaaag catactttat cactctgttc ttacacccat gctaaatatc
     1981 gggttgatta atccgcaaca gatcatagat aaagcaatac aagctgccat tgacaaggaa
     2041 atcccgttaa attcattaga aggatttatc cgccaaatca ttggatggcg ggaatttatt
     2101 cgcatagtat atgaaaggga aggcacccgc caaagaacaa ccaactactg gaaatttacc
     2161 cggaagatac ctcttggttt ttggaatggt attaccggta ttcacccaat agatacagtc
     2221 ataaaaaggc ttttaaagac aggttactgc caccatattg aacgattgat ggtgctcggc
     2281 aatttcatgc tattgtgtga gtttgatcct gatgaagttt accggtggtt catggaaatg
     2341 tttgttgaca gttatgattg ggtgatggtt cccaatgtgt atggtatgac ccagtttgct
     2401 gacggaggac tgatgacaac taagccgtat ataagcggca gcaactacct gctaaaaatg
     2461 ggtgactggc ataannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgcaga agaattctta aagagtcttg
     2641 attgatgaaa tctgtaaaaa aatcagacct gccgcaaaag atatgcatag tttgccaacg
     2701 tccgtttaca tggcgaaaaa agtggaaaca agtttgggaa gaagtaaaat actgcagtga
     2761 caaatgcagg atgaaccgcc acatatctgg ttaacgttta ctgatcttgt tacctgaaat
     2821 tgcccgctgc ctgctgcact taaaacggct tacaccggta attcttagtt tttgatgctt
     2881 ggtgcttctg cctgccattc tttttcgcca catagtaaag ctgcttcttt tcatatgccg
     2941 gcgcataagc gcaataacat ccttttctcc taatccaaac tgaacccata ttgcctcgaa
     3001 aggtgttctg tcttcccagg ccatttctac aaggcggtct atatccattt ctgtcaaaga
     3061 tttacctgtc ttttgcataa aatcctgaaa caacttgtta tcgtatatct atacannnnn
     3121 nnnnnnnnnn nnnnnnnnna ggcaggctaa ttgttataag tatatgcagc atattgaatt
     3181 ccccacttcg tattctgaaa ttcttaaaag aatagatgaa attgatccca ttgaatatgc
     3241 ccggtcaaga aattatttaa acggatcagt aacttacctg tcaccttata tttcaagggg
     3301 agtcatctca actaaaaaaa ttctgcaatc cattttagaa aaaggtttta ccccggcaca
     3361 atccttaaag tttatacagg aattagcatg gagagaatat ttccaaagag tatggcaaca
     3421 cctggaagat gatattttag atgacatcag aagaacccgt acaggtgtaa acaacaggca
     3481 gatatctgct gcgctaatta atgccgccac aggtatcgaa tcaattgata aaggaatcaa
     3541 tgagctatac gaaactgggt atatgcacaa tcaccttcgt atgtatgtag ccagcattgc
     3601 ctgcaacgta gcaaaatctt actgggatat gccctcccgg tggatgtatt atcacttgct
     3661 ggatggcgac attgcaagca atacctgcag ctggcaatgg gtagcgggca gtttttcatc
     3721 caaacaatat ttcgctaacc aggaaaatat taataagtac accgggagca aacagatcaa
     3781 tacatacctt gataaagctt atgatgagct ggcggagcaa aacataccag agcctctaaa
     3841 caaacttatt tcccctgtat tatctaccaa tttaccggaa aagaataccc cggtgctaaa
     3901 tgaacattta cccacactgc tctatnnnnn nnnnncttca tataacctgg atccggagtg
     3961 gagaacagat atggaagcca acaggatatt actattggaa ccttcgcact tccggcagtt
     4021 tccggtaagc gaaaaggttt tggccttcat catacaactg gcctccaata tcaagggcat
     4081 ccagcttttt gttggtgaac tgaatgaaat acccggactg aacaattcaa aagcgattta
     4141 ttcaaaagaa catcctgctt tcaaacattt tccgggcatc aaagtcgaaa gggattggct
     4201 atttccggaa ataaaaggct tttataattc tttcttcagt tactggaaac aatgtgaacg
     4261 atctttaagt aaaatggaga aaagagtacc tgcattgttg cgtgcctgaa aatactgttc
     4321 tggtacactt atctttgcga aaaataacac atggagcccc gcaaagcctc ggacagcctt
     4381 actataatga cggaattggt actacctaac gacaccaaca tctttggtaa tttgatgggt
     4441 ggccgtctta tgtattggat ggacatagca gctgccctcg ctacacaaaa gcattgtaac
     4501 gcaccagcgg ttacagcatc ggtagacaac atttcttttg aaagtccgat caagctggga
     4561 aacgtagtac atattgaagc taaaataacc cgggctttca actcttctat ggaggtacac
     4621 ctgaaagtat ggggcgaaga cctgattcat caatacaagt ataaaagcaa cgaagcctat
     4681 tacactttcg tagcactgga tcctaaccgt aaaccaagac ctgtaccccc ggtgattgca
     4741 gaaacggagg aagaaaagaa attgtatgat ggcgcccttc gaagaaggca gttaagattg
     4801 atactgggag gaaaactaaa gcccgacgat gctactgagt taaaggcatt attcgtaaag
     4861 gattgatcat ttatccgttg tcagccagtg atgcttaccc atcatcatgt gttttttact
     4921 ttgttcaatt gcatccatta cctgcgcaag tatgttctcg gctgaatctt cataattgat
     4981 ttggagaggt tctttaaatg ttactgataa caaggagcct ttctttttaa atttcaggcc
     5041 ctttttgtta aatgccctcc agaatccact aaccactact ggtattacta ctggttttat
     5101 ttgcttaatg ataagtgctg ttccttttct gccgggcgca aagggtttgg tggtgccctg
     5161 tggaaaagtg atcacccaat tattttccaa tgcccgggta atttttcttg tgtcagatgg
     5221 gtccagacct ctgcgaactt ctttgccccc ctcccgccat gttcttttta cagtcaatcc
     5281 gccagccagc aggaaaaggc ggctgataaa actgcctttc attgtttcct ctgctgccac
     5341 ataatttact ctcgtaaaag gattcagtaa gtagtaaggg actcccagcc tgttcatttt
     5401 tccccattta actgcacaaa agatatgcag gaaggttatg acatcagcaa aataagtctg
     5461 gtgattactt acaaacaaaa cccttcttct cggcaaatct ttcaagtgtt ctgtgccagt
     5521 aatttttaat ctattaacca ttgtaagacc cggatatgaa aatgcaccca caataaggta
     5581 tacgattttc cttaccgggt gaaaatgtct taacctgtct gaaatggaag aaataaaact
     5641 cattaaacaa gcaatctgga ttcaaattaa ggaattgtaa acgaaagaac gtcttacaga
     5701 cttgggattt attacaataa aaaacccttc tggtttacag aagggtttta atttctctat
     5761 cttaaaacgg aattattcag gctttgcctc ttcagatgaa gccgcttcgc cgctatccat
     5821 tttctttttc agatcagcca gtacacctaa gtcacccaat gtggcttttt ccactttgtt
     5881 ctgaacactc ttcacggctt tacgggtagt ttcagcttca gctcttgctt cttttttagc
     5941 agcttctgca gcctctactt gtccctgctc ccaaatgcgg gtatgagaaa ccacaattcg
     6001 cttttcattg cggtcaaact caattaccat gaactgggct gtttcatcag cagcaatgct
     6061 ctttccatct tccttgctca ggtgacggtt aggagcaaac ccttcaagtc cgtaaggaag
     6121 ctgaacaata gcacccttgt cgtctctgcg gcttacggtt ccctcatgaa tgctgccgat
     6181 tgcaaaagta tcctgcagcg tattccaggg atcttcttcc agttgtttgt gtccgagttg
     6241 cagtttgcgg ttctctttat caatgccaag gataactaca tcgatattgc tgccgacttt
     6301 agtatactca cttggatggt tgaaacgctt cagccagctc aggtcgctaa tatggatcat
     6361 gccaccgatg ccgggggcga gttccacgaa tacaccgtag ttggtgatgt ttttcaccag
     6421 gccggtgtgg cggcttcctt cagcaaattt tgtttcaata tcattccatg ggtcagcaga
     6481 aagttgtttg atggacaaac tcatcttgcg acttgcctta tccagtgtaa ctacttttgc
     6541 ttcgtactca tcacccaatt tgaagaattc cttcgcattg atcggtgtat tggcccatgt
     6601 aatctcagat acatgcacca gtccttcaac accaggctga atttccagga atgcaccata
     6661 atcttcaata ttcactactt taccctttac aatattccct tcagcaatat tatctgccag
     6721 aacatcccat ggatgcggag tcagttgctt cagaccaagg ctgatacgct tcttctcatc
     6781 atcgaaatca agcaccacca cattgatctt ctgatcaagt ttcagtactt cagaaggatg
     6841 agagatgcgg ccccatgaaa tatcagtgat atacaacaga ccatctaatc cacccaggtc
     6901 cataaaggct ccgaagtctg tgatattctt cacagtacct tccagtacct ggcctttctc
     6961 cagtttgctc atgatctctg ctctttgagc ttcgatatcg ctttcgataa gtgctttatg
     7021 agaaactacg gcattcttga ttgtctcgtt gatcttaaca accttgaact ccattgtttt
     7081 tccaacaaac tgatcgtaat ctgtaaccgg cttcacatcg atctgtgaac caggtaagaa
     7141 tgtttccata ccgaagacat caacgataag accccctttg gtcttggaag taacagtacc
     7201 ggtaataact tcacctgttt tgtgtacctc aacaattttt tcccatgcac gggtaatgcg
     7261 ggcctggcgg cgactcaggt tcaggtgtcc ttccctgtct tctttttcta caatcatcac
     7321 ttctacttca tcaccaacag acaggtttgg aatgtcgcgg aattcattca atgaaatnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtgata
     7501 tttctctctt tcttcagcag aataagaaat cacattgcgc ctgtcaacac tccagtcaaa
     7561 atcatcatga gcagtagcta ctgcttccac aggtgcagtt gttgtcgctg tagttacttc
     7621 agcttgttca gcggaagcag actcctgtgc atcagcgttt agttgttttt taataatgtt
     7681 atcaaacatg ataaatagat cccgaagggt tttatttcgg ggcagcaaag ataagttata
     7741 gttccggctt tagggaggtt gaagtgagct atttttttga tacggaagcc ggctgcaaca
     7801 ccttgattta gcgttccttg cgacgctcct ttcagggttc tggtcgctgt tcatctgtaa
     7861 atattcctat attcaccaaa aataggcctt gctgtataaa ttgttgaaaa tcctgtcccg
     7921 actcgcactg ccgatatggt gtcgaaggct gaccactaac aaaccggaaa tattaaaaga
     7981 aaaggggcca ttgctgttag ccagcaacca tcccaattca ttccttgatt cagttttact
     8041 ggataccgtt tttcaacaac cagtttggtc gctggccagg ggagatgtct ttaaaaaacc
     8101 tttttacata aaattattga ccgctttaaa gatcctacct gtttacagaa caagtgaagg
     8161 agtagaaaac ctttctgaaa attataaaac atttgaagag tgcattacta ttttcagaaa
     8221 aggcgggata atcacaatct acagtgaggg caaatgcatc aatgaatggc acctgcggcc
     8281 tttgaaaaaa gggaccgcaa gattagcggt taaagcatgg gatgaaaata ttcccctgaa
     8341 aatagtcccc gttgggatca attacagttc atttagcagg ttcggaaaaa atatcttcct
     8401 caactttggt gaaataatca cggcgaccga tattattaat ggaggtgcag acgggcaacg
     8461 gcaccttgct tttaataaca aactgaaaag tgaacaggaa aaactggtgt ttgagatacc
     8521 tgcgggtaat aaagagctta gacaagaaaa gctggcagta aaaatttcat tggaaaaacg
     8581 aatacttctt tcattgccgg cattgcttgg atggctgttg cacctgcctt tgtttttacc
     8641 agtcaaacaa ttctccagac aaaaaaccaa aggcacagat cattacgact ctgttataac
     8701 aggtatttta ttaattatct atccgtttta cctgattttg atttcagtat ttcttttcct
     8761 gataaccctc agttggtggt acctattagt gcttacggta tttcccttta cagcatgggc
     8821 atttgtgcaa ctaaaaccac aactggatta attcaaccgg ccgcaattga ctttgccgca
     8881 atgaatccgc tggtccatgc atgttgaaaa ttaaaaccac ctgtaacacc atccacatct
     8941 ataatttctc cagcaaaata caggttactg acttttttgc tcatcatcgt gttatgatca
     9001 atctcgtaaa gttttatacc cccggctgtc acgaattctt ctttaaaagt agtttttcct
     9061 gtcacctgca gttcataatt gcatagatgc ttaatgaact tattctgaac aacagatggc
     9121 atttcagccc agcgcattgt ttcagtaaca cctgattgca tagccaaaaa ttcccataac
     9181 ctccccggca gaccatgcat attccttcct ttgatccgct gagaggcatt ttcattcctg
     9241 atttgtaaaa atctttcttt caaggtttgc tcattcagtt ctggcaacca gttgatggat
     9301 attgtaaact cataattgag ctttgccagt tccctggcgc cccatgcact taactttaat
     9361 acaacaggac cactgaggcc ccaatgtgtt atcaaaactg ctccggattc tgcaagtttc
     9421 gattgctgaa ttttcacttt cacattttct gcacttattc ccatcagctc cctgataggg
     9481 tggcctggca aattaaacgt aaataaagag ggaaccggct cttcgatagt atgacctgtt
     9541 tctttcagcc agtcaaacat agatgacttt gggtagccgc cgctggcaac acatacataa
     9601 tcagcattga gctggcggtg atcactcaac tccacatgga atttctcctg agttttttcc
     9661 agtttcttaa cttcggcatt aagcattatt gctacattgt acgtactggc ctctttcagt
     9721 atacaacgaa taatagtttc agaagtattg ctaacaggaa acattctgcc atcctcttct
     9781 gttttcagcg ttactcctct ttcatcaaac catttaatag tgtcagtagt aaaaaaatgg
     9841 tgaaaggttt ttttaacaaa atttgctccc cgaggatagt ttcggctcat ctctccaata
     9901 tcaaagcagg catgagttac attgcaacga ccaccaccgc ttacttttac ttttgagagc
     9961 agcttgttag acttttcaat aacaataact ttaagtaacg gatttagtct tgcagcatta
    10021 acggcacaaa aaaaaccagc agcacctccc ccaattacta tgagtttctt ttccgccatt
    10081 ttttaaaagt gaacccctca gctgaggggt tgaaaaattt tggcagtaaa caaattggcg
    10141 ttggccgctt ctgccttctc aatagggcta taatcagaga gaatgtcagc cttcgacttc
    10201 ctgacacata catcatgttc aaaatgtact gatggctttc catccatggt ccttactgtc
    10261 cagccatcag attctgtata tacatccttc ttgcccatat tgatcatggg ttcgatggtg
    10321 aagcacatgc cggggaggat ggtgtgtttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacaccata accatgtttt
    10441 ttctctgtgt gttcttgtat tgcaaaagca atatcaccaa ccctgtttcc agcaattgct
    10501 ttctcaattc ccttgtacaa ggattgtttt gtgaccagaa taagctgcat tacctctggc
    10561 ccgggatcgc caatggcaaa agtataggca tgatctccat gccagccatt taaaatgact
    10621 cctacatcta ctgaaacgat atcaccttct cttaattcat tatcacccgg gaaaccatgc
    10681 actaccacat cattcactga aatacaggag ttataaggat agccgtggta gttcaggaaa
    10741 gaaggaacag catgatgatc cttgatatat tcgccaatca tcctgtcaat tgaaagtgta
    10801 gtaatacccg gctttaagtg ctttgccact tctgtcaatg ttttgctnnn nnnnnnnnnn
    10861 nnnnnnnnna aagcactctg ccgcatcatt tctacctgtt catttgtttt acagatcatc
    10921 atatcctgca aatatcttta aaaaaacaga cccggcaagg attgcttacc gggtctgcca
    10981 atatcattca atttattaca tttgaacggc agaagtagtt tgtctgccct ggatgcgtcc
    11041 gctcttcatc aatccgtcat actgacgcat caataactgt gtttcgattt gttgtaatgt
    11101 atccaatatt acacctacca tgatcagcag tgaagtacca ccaaagaagg tagagaactg
    11161 ctgcgttaca ccaaaacgct gtgctatacc ggggagaata cccacaaaag ccagcaatat
    11221 agcgccgggt agtgtgatct tatccatgat ggctccgata taatctgctg taggctgacc
    11281 aggttttaca ccagggatga aaccattatt ctgcttaagg ttatcagcaa tttgcttagg
    11341 attaaagatc agggctgtgt acaggaaagt aaaacctatc accacaatgg agtagataag
    11401 catgtaccac acattactgt gatcattcaa tgctctcaga aaactaccac caccagttgg
    11461 gttaaagttg gcgaaaatgg tagggatgaa tatgatcgcc tgtgcaaaga tgatgggcat
    11521 tacgccggca ctgttaactt tcaaaggcaa aaattgtctt gctccgccga actgacgatt
    11581 accaacaatt tgttttgcat agttaactgg tacctttctt acaccctgta tcaacaggat
    11641 acatcccatg ataatagcaa taagaatggc aatctctaca atgaatacta atatctcgcc
    11701 attacgaaca gatttggcac tcaattcctg aaggaaggat tgtgggaggc gggccaggat
    11761 accgatcatg atgatcagtg atgtaccatt acccaatcct ttatcagtta tcttctcacc
    11821 catccacatt acgaaaagtg taccggctgt caataccact actgttgaca accagaaaaa
    11881 gttggcataa gcaggcatga taccaccagc ctgtgtcata ccacgaagat aggtaacata
    11941 agcagatgcc tgtaaaatcg taacggcaac agtcaggtaa cgggtgtatt ggttgatctt
    12001 tttacgaccg ctttcgcctt ctttctgcat tttttgaaac cggggtacta aaactgtcat
    12061 caactgcata aagattgaag cagagatgta gggcataata cctaatgcaa aaatagaagc
    12121 ctggttaaag gctccaccgg agaatgcatc aatcaatcct aagataccag tactgttctg
    12181 accactttcc aaagcattgg gatcaatacc cggcaataca atatgtgtac ctacccggta
    12241 aatagcaatc atcatgaggg tgaacaaaat cttgccacgc agctcttcaa tgctccagat
    12301 atttttgagt gtttgtatta atttcttcac ggggattaat aaaagtttaa gtgctcttaa
    12361 aacataaaag acgcattttc aatgcgttgc tgcaatttag tgattttact tcactatttc
    12421 gacagaccca ccggctgcct cgattgcaga tttggcttta tcactaacag catttacttt
    12481 gaaagtgaat ttgcctttta tttctccatt acccaggatc tttactctgt cgttctggct
    12541 aaccagtccg ttaatgtata gattctcgag agatacttca gtgaaattgt acttttcagc
    12601 taattgttct atctgtccga gattgaaaac tttgtactca acacggtgag gatttttaaa
    12661 tccacgtttc ggtatacggc gctgaatagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntctac
    12961 tttcaccagg tgggtcacct tgcggagcat acccattatt tggggagtag cctccacttc
    13021 tttagaagag ttggttttat taagacctaa agcctgaacc gtcagctttt gacgctctgg
    13081 tctgtcaata ggacttttta ctaaagtgat ctttatcttt ttcataattc aaattagttt
    13141 gaactggtat taaccgttga atactttctt taaaccaagt gtacgggttt tggcaacaga
    13201 aaccggctca cggagcagtg caagagcttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgatga
    13381 gaactttggc agcgccttct ttcgcccact gatcatgagg tatggttcct ttcatcaccg
    13441 gaactttgat gaggttcttt ttggcatctt caattccttt agtgatggct tcctgaactt
    13501 ctttggcttt gcccaaacca tgacctacca caccattttc attgccaaca accaccagtg
    13561 cagagaaact gaaggcacgg ccaccctttg tagtttttac cacacggttg atggcaacaa
    13621 ccttatcttt cagttccagg tcaccggctt ttaccctgtt tacgttaagc tttgacattt
    13681 atacttgtaa ttaaaagttt gatagtaaat attagaattc caacccacct tctcttgcgc
    13741 cgtcagctac agatttaaca cggccatggt atagataacc accacggtca aatgttacat
    13801 ccttaatacc caattcagag gctttgcggg caatggcagc tccaaccagt ttgctctttt
    13861 cggattttgt aactttctgt gctgctatgt ccttatctct tgatgatgct gacgccagtg
    13921 tctggccttt tgtgtcatca atcaactggg cataaatatc actgttactt ctgtatacag
    13981 acaggcgggg ctttacagta gtaccactta tctttctgcg tattctgtaa cggatatttt
    14041 gtcttctctg tgattttact ttcgggttca tctctctttt tatttgtgat cctgattctg
    14101 ccaggagtca attacttatt aattatttac cagccgcttt accagctttc ttacgtacca
    14161 cttcacccac ataacgaaca ccctttcctt tgtaaggctc aggcttacgg aggcttctta
    14221 attttgccgc cacctgtcca attaattgct tatcgatacc ctctaatgta atgatggggt
    14281 tttgaccttt taattgttcg gttgccactt tcagttcttt aggaacttca aaaatgatat
    14341 tgtgagaata tcccaaagca aggtcaagca cattgccctg gtttgcagct ttgaaaccca
    14401 caccaatcaa ttccagtttt ctttcaaaac cttctgtaac acctttcacc atattagaga
    14461 tgagtgaacg gtacagacca tgcattgcac ggtgacgaat ctggtcggta ggacgggcaa
    14521 aagtgatctg tccgtcttta acctcaacag cgatatcacg gtcgatggcc tgcttcaact
    14581 cacccttggg tccttttact gtaaccacat tgtcctttcc cactgatatt gttactccat
    14641 taggggcaac aaccggtttt tttcctatac gagacataat ctgtttttta tttggttatc
    14701 gtttactgac cggtcagctt agtatagctt cagcttaatg tatcagtgaa aggaatgcgg
    14761 cttaatgtat tacaactcat ttttaatata cgtagcaaag tacttctccg cctacattct
    14821 gtgctttagc ttctttatca gtcattactc cctttgaggt agaaatgatt gcaataccca
    14881 ggccgctctt tacacggcga aaatctgcag gcttggaata ttgacgaaga ccaggacgac
    14941 tgatcctttc catgctttga atagccggtt gcttggtagc ggggtcatat ttcaaggcaa
    15001 tcttgatgat gccttgcttg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggataattgg aaaagtttag
    15241 tttaattaat taattgttta ccagctggct tttttcaatc ccggaatttt tccgttcagc
    15301 gccatgtcac gaagtgcaac cctggagata ccaaagtagc ggacataacc tttcggacga
    15361 ccagtcaact ggcaacgatt cttcaaacga acctttgaag agttctttgg catttcgtca
    15421 agagctttcc agtcaccagc tttcttcagc tcttctcttt tagctgcatg ctgggcaacc
    15481 atacgttccc tttttctttg cctggctttt actgatgttt ttgccatgtg tatattatcc
    15541 ttttagtttg aattattttt tgcattcttg aaaggcatac ccaattcctt caacaactca
    15601 taagcctctt catctgtgtt ggcagttgtt acgaatgtga tatccatacc ggtaatctta
    15661 ttcactttat cgatatctat ctccgggaaa atgatctgct ccgtaacacc cagtgtataa
    15721 ttaccacggc cgtcaaaagc tttttcattt actcctttga agtcacggac acggggaagt
    15781 gctacagaga tcaggcgatc aagaaattca aacatcttgt caccacgaag tgtaactctt
    15841 gcgccgattg gcatattctt acgcaactta aagttggaga tatctttctt tgacatggtg
    15901 gtcaccgctt tctgaccagt aatggttgtc agttcctcta cggcgatatc aaccagcttt
    15961 ttgtcagaaa cagcgccatt cacaccacgg ttcagacata ttttttccag cttgggcgcc
    16021 tgcattacag tagcatagcc gaattttttt accagggcag gtacaacatc ttttttgtac
    16081 ttatctgcca gtcttggggt ataagtttta gtactcatta ttttttacct ccctgagttt
    16141 ttttagtttt aaaaactctc tcagtttttc cgtctttcct tacccttgta acccttgcgc
    16201 cggctttctg ggcaggatcc caaagcataa cattactgat gtgaatagga gcctctttct
    16261 ttacaatacc acctttggtg ttttgtgcag aaggctttgt atgcttagtg atgatattca
    16321 caccttctac gataactttc gcttcatcaa ccaacacctg ctttacagnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnaggtct ttatcatcgc cggcgataac tactacattg tcgcctttgc
    16441 gaatgttaaa cttgggtttg aatcttgctt tcatgtttgt ctatcattaa tgggccgcag
    16501 ccccttttta ttcaattata atacttccgg tgccagtgaa ataattttca tgtaaccttt
    16561 gtcgcggagc tcccttgcta cgggaccgaa aatacgggta ccacggggct cgtcagcctg
    16621 gttaaggatc accactgcgt tgtcatcaaa acgaatgtat gagccatcct tacgacgaag
    16681 cttattggta gtacgaacga ttacagcttt tgctactgta cctttcttga taccaccctg
    16741 gggcaaggca tcttttactg ttactacaat cttatcgcca atcctggcat aacgctgacc
    16801 gctgttgccg agtacacgga tacaaagcac ctctttggca ccactgttat ccgcaacatt
    16861 caaacggctt tcttgctgaa tcatcttttt gtctgtttaa tattttatga ttgatgactg
    16921 aatatcggat agtagctcaa tattcattct caatatccaa ttacttaact ttctcaacta
    16981 cttccaccag cctccagcgt tttgttttgc tgagcggacg ggtttccatg atcttcacta
    17041 catccccgat gctgcactca ttcttttcat catgagcatg gaacttagtg gttttcttta
    17101 cgaactttcc atagatcggg tgcttaactt ttctttctac agcaacagtg atggttttag
    17161 tcatcttatt gctggttacc acaccaatcc tggtttttct taaatttctt gcttccattt
    17221 ttatagcttt attatgcttg ctgttgcttt aactttaatt ccgttttcag gcgggcaata
    17281 tccctgcgca ggccacggat ggtcattgga ttctccaacg gagaaatagc atgggcaaat
    17341 tccagcttct ttaaacgctg cttatcttct tcgaggcggg ccttcaggtc ctgctcgttc
    17401 atcccatgta tgctttttac aaaatctgct ttctttgaca tcgctttacg atttttagtt
    17461 attatgcagt aaccaggtca cggcgtacaa tgaacttagt tttgattggc agcttaccgg
    17521 cagccagggc taatgatgca cgggctacct gctcactaac accatccact tcaaataaga
    17581 tacgacccgg cttaactact gctgcccaaa actcaaggtt acctttaccc ttacccatcc
    17641 ttacctcaag aggtttgcgg gtaatcggtt tgtcagggaa aatacggatc cacacattac
    17701 cctctctctt catttcacgg gtaagggcct gacgggctgc ctcaatctgg cgatcagtga
    17761 tccaatgact gtccagagct ttcagagcat aggaaccaaa agaaatagta gtacctcttt
    17821 ttgcatcgcc tttcatgcga cccttctgca tcttcctgtg cttcgttctt ttcggttgta
    17881 acattgtata taatttctag ttacttaaat cttgtttcta attagcttct tctttctctt
    17941 ccaccacctc tcctgtctcc tcttctgtca tcacgacgtc cgccgccacc accttcatcc
    18001 cttctttctc ttctgtcact tacatcactc ttacctccta cgaagttggg attaagatct
    18061 ctttttgtca gtacctcacc tttacatatc catactttga taccaatttt accgtaaaca
    18121 gttaatgcaa acacattggc ataatcaata tccatacgga atgtatgcag gggtacacgg
    18181 ccttgtttga tctcctcact acgggcaatc tctgcaccac cgaggcgacc tgatactttc
    18241 acttttatac cctcagctcc catacgcagg gcagaagcga tggacatttt aatggccctc
    18301 ttgtaattga tacggttttc gatctggcgg gcaattgtat ctgcaacgat cattgcatcc
    18361 agctcagggc gacgaatttc caggatattg atctgtacat cttctttacc tgtcaacttc
    18421 ttaagctctt ctttgatacg atcaacctct ccaccacctt taccgatgat gataccgggt
    18481 ttggatgtat gaatcgttac gatcagttta cccagggttc tctcaataac aattttagag
    18541 ataccgccct tattgatacg ggcattcagg tatgttctga ttttattatc ctcgatcagt
    18601 tttccagcat aatctttttt gctgccatac cagttagatt cccatccacg gatgatacct
    18661 aagcggttac caatcggatt tgctttttga cccatgttat tggtttatta attcgtttag
    18721 tttttatttt ctttagtatc cactattaca gttacatggt tgctgcgctt gcgcaccctg
    18781 taaccacggc cttgcggagc cggacgcatt ctcttcaatg tcctggcacc atctacaaaa
    18841 atggttttga tcaccagctg gctttcgtca ccaccttcat ttttttgctt ccagttgctg
    18901 atagcactca gcaccaactt acgcagggga actgcaggat gcttgggatt atgctcaagt
    18961 tcggccaaca ccttctccac tttctgaccg cggataaggt cagcaagcaa ccgcatttta
    19021 cggggcgatg tcgggtaatt tctcagttta gctactgctt ccatttttat taatttcttt
    19081 attaaccttc ttttttagag tgtcctttaa actgacgggt aggtgcaaac tcacccagct
    19141 tatgaccaac cataaattca gttacataaa cagggatgaa cttattgcca ttgtgaacag
    19201 caaatgtgtg acccacaaac tcaggagtga tcgtagagcg gcggctccat gttttgataa
    19261 ctcccttctt gtttttgcct tcgtttatag ccagcacctt tttttcaagg tgagatgcta
    19321 cataaggacc ttttttaatc gaacgagcca tgatttatgt cgttgtgttt ttaattagtt
    19381 tgtttactta gccagtttgc ttccgttctt acgctgaata atcagtttat cacttccttt
    19441 tcctttggtt cttgttttca aacctctgga gtactgaccg ttcctgctgc gaggctgacc
    19501 accggaagct ttaccttcac caccacccat cgggtgatct acagggttca ttgccacacc
    19561 acggtttctt ggtttaatac ccttccacct gttgcgtccg gctttaccca tgctctgcaa
    19621 actatgatcg ctgttactta caacacctac agttgcatag caattgatca atacttttct
    19681 taactcgcca ctgggcattt tcagtacggc gtatttttct tccttgttgg taagctgggc
    19741 tgatgaaccg gcgctacgag ccagtttacc accctgacca ggttgcattt caatattgtg
    19801 aatattggta ccaagcggca tattcttcat aggaagagca ttacccactt caggagctac
    19861 atccggacca ctctccactt tagcgccaac ctgtaaaccc tggggagcaa ggatataacg
    19921 cttttcgcca tctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnncata gacaagtgac cagaagagtt acggccaccg gttcttggct
    20101 ttgcctctaa caggcttttc tccggtacat tcgttgtaac ctcagcgtag gcattcccga
    20161 ttctccagcg ggtacctgcg gtcattggtt taaattttct tagtgccatt ttaaatttcc
    20221 ttttttagtt caacattagc ggctgttgaa gccattctta atacgggtta gatatttgaa
    20281 taaagatcaa tgttctcacc ctcagctact gttacaaaag ctttcttata gctcggtttg
    20341 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnga tctccagttt atttgccttt ttagccactt tgaaagcata gcgacgcagt
    20521 ttatcctgct ggctgtttgc tttttcggtc aaaatgggct ttatcaatac ttcagaaagt
    20581 ttcattgctt tctactttaa ttattttctt ttaatagctc ttttattacc ctgagcttgt
    20641 cgaagggttg attaagcttc cgctttttca tcatcagcaa aaatctttgc tgcactctca
    20701 gttaatacca aaacctcaga gtttacaata tcatatgtgt tgatatcgct gagtaaaacg
    20761 cccagtacag aaggcacatt gcgcagtgaa agctccacat tatcattgta ctcaggctga
    20821 acaaacatta cttttttatc cgcaacttta aggttaccca ggatgtcaac aaacgttttt
    20881 gtctttggag tatccatgtt gatatcttca accaccatga tagcattatc ctttgccttg
    20941 taagaaagag cagagatttt agccaggtct ttcaccttac ggttcaattt gatatcataa
    21001 ctgtgaggct tgggaccaaa aatggtacca ccacctttat ataaagggtt acggatatta
    21061 cccttacggc tgccaccagt acctttttga cgatgcagtt tacggctggc gccttttact
    21121 tcagcacggg tcttcacctt gtgagtaccc tgacgctgtg cagccaggta ttgtttcact
    21181 gccaggtaga tcacatggtt gtttggctcg gcaccgaaga tttcttcggg cagttcaact
    21241 gttctgcctg ttgcttttcc tttgctattt aaaacttcga tttgcatttt tattcgtttt
    21301 attagccatc cttcgacaag ttaaggatga catctattac ttctggatta atacaattga
    21361 accgttatga cccggtacag aaccacttac caggatgtag ttcttatcag cgaaaatttt
    21421 aactactttc aaccctttca acttcacacg gtcattgccc atacggcctg ccatacgcat
    21481 acccttcata acacggctgg catcagatga gttacctaat gaacccgggg ctctctggcg
    21541 atcatgctga ccgtgagatt gctgtcccac accatggaat ccatgacgct ttaccacacc
    21601 ctggaaacct ttaccttttg tagtaccaac tacttcaact ttatcgcctt cagcgaaaat
    21661 gtcgacggta actgtttcac caatattttt ttcaattgcg aagtcgcgga attcttttga
    21721 gaattttttg ggagctgttt gtgcttttgc gaagtgattt ttttcagctt tagtggagtg
    21781 tttctctttc ttgtcgccga aagagagctg aagtgcattg tacccgtctg actcttttgt
    21841 tttaacctgg gtaactacgc aaggaccggc ctcaataatc gtacaggcgg tttgcttgcc
    21901 ttccggatcg aagatgctgg tcatcccaat tttttttcca ataatacctt tcattnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt attgggacaa ccttgtacat tccagacttt
    22021 ataagtctgg aaggcttcaa tccccgtttg ccaccgacct tttcctttat ggggaagtac
    22081 cggcagtaaa caaaccctga atggcttgtt ttatgttaat gcgccagagc tcttttctat
    22141 aatcccggtt tcaccgggat atttttgaga gatagcaatt atttataatt ataagaacta
    22201 attgaggcga acctcagttt ctacgcttta atttcaactt ccacaccact tggcaaatca
    22261 agcttgctca gtgcatccac agtacggctt gaagaagtgt agatatccag caaacgctta
    22321 tgcgtagcca actggaactg ctcacggctc ttcttattta cgtggggtga acgcaataca
    22381 gtaaaaattt tacggcgtgt aggaagggga ataggacctg ttactacggc accggtactg
    22441 cggactgttt ttacgatctt ttcagcagat ttatctacca ggttgtgatc gtaagactgc
    22501 aattttattc tgattcgctg cgacatgtgt gaaatcccaa tttttctttt gggagtgcaa
    22561 aggtagggct gagagctata gcttccaaat agttgggaaa gaattttata ggatgttggt
    22621 aattagtttc tcaataagca aatatcgcac agttgtttga ttatcaatcc tgcagaaaat
    22681 cagagcttgt gtctatttga taagcaagct attaactgcc cgcaatataa attggtcaca
    22741 atcacataca aacaggatga tatcaggcta attaattgat caattaaaat tattactgtg
    22801 aattatcatt cataaatgcc gttggctgtg ttgacttacc gtccggcacc aattcgttac
    22861 cagtttgtgg tatcccttaa cattcattaa cactaacacc tgttattcac acagttatgt
    22921 ggtatttcaa aagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagaa
    23101 gcgaattatg cttttgacaa aatcaatgaa acaagcagca caattcatca atacctgggc
    23161 caggtgaagg aaatattaga ggcaattatt acaatggccc cctatatcgt tatggaactg
    23221 gaaaaaggga acagctcggt aaaggaactg gtaataaagg cagctactat tcgtgaccaa
    23281 tatagtcagg cttttacatt gtccctgaaa aggaaaattg gtaatgaaat gcaacaactg
    23341 ttttttgagt tgaccgctgc atcgttgaaa atgatgaacc gtgaggacat gatcgtaaat
    23401 gacctgctgt ggaccaacta cagtgactca gaaatacagg aactggaaaa gagaatgacc
    23461 gctccttcat tcaaaattga aaaagaagtt gctgccgttg agcctaaagt ggccacagaa
    23521 gtttctctgt catcagtaaa tattactccg ctgatttcat tgagtgatat actcccggtc
    23581 agtgaaaaat tatcaatgct tattagaaga gtcctggcac cgggtaaatg gagatttgca
    23641 aaccgggtgt ataatattat caggcctcac catttatttg gtataatgcc ggcaattatt
    23701 tgtaccacaa tgtttgaatt atcggcctga tctttacact cagtgtagca aaaccaccca
    23761 actcaagatg ttccacccgt aagcgatgga atccttcaag atccaacctg atctttctat
    23821 taggtgattc gtcaaatttg tattttgaag ggttccattc ctccagaacc agcttttcgt
    23881 caaggtaaat ccttacagca tcatcccagg taacggacaa ttcatatgtc cctttgggaa
    23941 cggtgaaact gccattcgct gaggtaataa actgcttgtg ctgaaatgaa ccttctctga
    24001 ttccacccca ccaggtatag tcaagtctat tggtcttttc tgtcttgaaa ggtgcctttt
    24061 tttcatatat agagaaaagt gatccctcct ttattggatt atgactattg gtatccaggc
    24121 tgaagaataa taactcccaa tcaaccggct gaaagaattt ttcaaaataa aaccgtgatg
    24181 gtttgcctgc tgcaattctt tttccaaact gatttgtata agacattcca ttatacccca
    24241 gttcaataaa aatatctgtc ctctcacctt ttattttttc agctgtaaga accgccggga
    24301 atgttccatt catcgctgaa atattgtgta gtccccagaa acgtatgatt ttccagctac
    24361 ctgctggtgc tattaaatca aatttcatca tcggtgaaga atcggttgga ttagtattcc
    24421 agataaccgg gtatccaaaa tcatacggtc cccactcatt aaccataata ttcttccggc
    24481 ctgaatattt gttgctttct ttaaaaggat tacctggctt tacattaagc gtatcaatgt
    24541 accaacccgc aaaatcaacc aatggtcttt cggaagaatc aatttctgtt acagttgaat
    24601 caattttcaa ataagctgca ctgcctgaat atgtatttcc aaatattttt actgccgatg
    24661 tgcgggaaat atttaatcca ttagctacat tattgaagct attcccggct atagtatact
    24721 ctctgctccg ggtatctctt ttcaatgcat agatccaatc agcaggctgc tcctttcttg
    24781 cccaaagcct gatggcctcc ctgtcttttg aaaagttatt ataccagatt tggttatcct
    24841 gcccatgctc gatggcaata ccaattctat tattcctgac tttgttatcc ttaatgattg
    24901 tagaataac
//
LOCUS       DAORLS010000011        24142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1152_length_24142_cov_2.018724, whole genome shotgun sequence.
ACCESSION   DAORLS010000011 DAORLS010000000
VERSION     DAORLS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24142
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1152_length_24142_cov_2.018724"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="PK977_01905"
     CDS             complement(<1..1548)
                     /locus_tag="PK977_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRF16885.1"
                     /translation="MRLLKYSVLTEEYFSALFWKDMLSPDRVTKEKISCYHCGETCPN
                     DKIQLEEKHFCCEGCKMVFQILNEHSLCDYYSLNENPGLSQRIKVRKDKFAFLDDEKI
                     QLQLVSFKDNKQIHVTFYLPQMHCSSCLYLLENLHRLDPGVVSSKVNFTRKEVEIVLL
                     TANTSLRKVAEVLTSIGYEPYISLNDLKEKRPAINKSMVYQLGIAGFAFGNIMLMSFP
                     EYLGIDASETGLREIFRWLNFGLAIPVLLYSALPFYQSSWRSLKHKFLNIDAPIALAI
                     IITFVRSAWEVLSGTGGGYFDSMTGIVFFMLAGRIVQDKTYRQLSFERDYTSYFPIAI
                     SVLKDNKEVPTTLPEIKPGDTLLIHNEELIPADGILTKGKAFIDYSFVTGESLPVLKE
                     VGELVYAGGKQTEGNIEILVVKEVAQSYLTKLWNRDEFRQEETKSVSFVHLLSRYFIY
                     VVLTIALGTAIYWQINDPSRIWPAVTAIFIIACPCALLLSNTFTNGNILRILGKNRFY
                     LRNAQTIE"
     gene            complement(1567..>1999)
                     /locus_tag="PK977_01910"
     CDS             complement(1567..>1999)
                     /locus_tag="PK977_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16886.1"
                     /translation="GTHITVAHAMGTTIGINTMLLFASVFYIIQNENKPVFERSKPTI
                     GWGILITNVSLLLFWSSLLGSGLLKISAKLNNEVFASIMQKSQPWFKLFAYSGIAIFV
                     GLFLLIFTVYKTLAIYSQKKQSVEEELVTIHSLHKVSEERT"
     assembly_gap    2000..2020
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2021..2986)
                     /locus_tag="PK977_01915"
     CDS             complement(<2021..2986)
                     /locus_tag="PK977_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRF16887.1"
                     /translation="MLTNSSQYASDKTAKAFLLLSISYLFFGLLLGIIGGIQYILPPF
                     LKEQLAFQKTRPLHVYLVISWIFTAAQAGLYYYLPRIANRPIYWLQGARIHFYLHALV
                     SFFIVACFFLGYFGGREYLEFPPLLGAVILLSWVPFAINFFFTLKPDYKNAPVYIWSW
                     STGILFFFITLSESYLWLLDYFHTNIVRDVTVQWKALGSMVGSWNMLVYGTGMYVMEK
                     MSGDNKANRSPLAFFFYFLGLTNLMFNWGHHTYIVPAAPWVKTVAYVISMTELLILGY
                     IIMKWRKTMTQARKNYHNIPYRLLSYADIWIFLNLSLAIVISVPSW"
     gene            complement(2993..>3186)
                     /locus_tag="PK977_01920"
     CDS             complement(2993..>3186)
                     /locus_tag="PK977_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16888.1"
                     /translation="GYLGPELTTAYSDPQRGEIYMKAFLQAGGPRMPNFHFKADEIDA
                     IISYLKYVDSTAVTYKSMP"
     assembly_gap    3187..3203
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3204..3393)
                     /locus_tag="PK977_01925"
     CDS             complement(<3204..3393)
                     /locus_tag="PK977_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16889.1"
                     /translation="MIKIAVFLILFTSYAIYSTVIYSKGTESGIQLTGPQAEKVSQGK
                     YLYEQYNCVACHQLYGLGG"
     assembly_gap    3516..3583
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3584..4327)
                     /locus_tag="PK977_01930"
     CDS             complement(<3584..4327)
                     /locus_tag="PK977_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRF16890.1"
                     /translation="MMKVSKYVLYDILRSKIVLAYTLFLLIVSLSMFNMEENSSKAML
                     SLLNIVLIVLPLVSLVFTTIHYYNSYEFIELMSSQPMSRTRIIISEFAGVSVSLLSAF
                     LVGVGLPILLYAPDETGFALLLTGMALTVVFVAIAFFASVRSRDKARGIGAALLLWFY
                     FTLIFDGLILLMIFNLSDYPLEKITLPLISLNPIDVGRIFVTLKMDISALMGVTSALY
                     KDFFTSSTGLLFTIGIMLLWTVLPLWLAIR"
     gene            complement(4339..5106)
                     /locus_tag="PK977_01935"
     CDS             complement(4339..5106)
                     /locus_tag="PK977_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF16891.1"
                     /translation="MISIEHISKRFRKLQVLEDINANMEKGQVISLIGPNGSGKTTLI
                     KSILGMVRPDKGKITVDGHVISGDHSYRANIGYMPQIGRYPDNMRIGQLFRMIENIRG
                     VAADKQDETLIRQFELEKIYDKPMRTLSGGTRQKVSAALAFLFDPAILILDEPTAGLD
                     PLSSELLKDKIIAEKKKNKLILITSHILSDLDELTTHVMYLQEGKLQFFKDMQTLQEE
                     TGELRLGKAIARIMKGEKLTNEWIDKILEVKKDEKIY"
     assembly_gap    5194..5287
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5288..6376)
                     /gene="nosD"
                     /locus_tag="PK977_01940"
     CDS             complement(<5288..6376)
                     /gene="nosD"
                     /locus_tag="PK977_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRF16892.1"
                     /translation="MNCIKISNKMHRLALILLLFLSANGFSRNIIVGKNQPVSSIRKA
                     IEIAKDFDSITVLPGVYKEGALQLTKSLTIIGQNYPILDGENKYEILLISGQNITVTG
                     LAFRNSGYSALNDYASIKLVDASKVTIAANKITNAYFAIHVSNTSYATIKNNNITGTP
                     TSEQLTGNGIHLWKSRYALIENNTIQGHRDGIYFEFVTNSTIRNNHSEKNIRYGLHFM
                     FSNDDAYLNNTFKDNGAGVAVMYSKKVKMENNHFDQNWGPSAYGILLKDISDSYISHN
                     TFYKNTVGIHMEGTSRMTIERNKFKENGWAIKVQASCENNSFQRNNFTGNSFDVGTNG
                     SLSLNEFSNNYWDKYEGYDINRDGVGDVP"
     gene            complement(6351..7382)
                     /locus_tag="PK977_01945"
     CDS             complement(6351..7382)
                     /locus_tag="PK977_01945"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRF16893.1"
                     /translation="MNNKLSVASRILVAFASGALIAVFFLPAWRIDLFAPQYPEGLTM
                     NIWINGLTGDVDIINGLNHYIGMEHITVNMFPEFKYLPYVVGFFMLLGMIVAITGNRK
                     FLLAYIGLTVLGGILAMWDFYQWGYKYGHNLDPKAPIQVPGMSYQPPLIGHKRLLNFD
                     SYSFPDVGGWIVIGAGCLAFGVWFAEWYKNRKTSKKLKTTTFAAPVIILCLFFSSCSA
                     KPEPFKYGQDNCHFCKMGIMDPKYGCEVVTKKSKVYKFDDLVCMINFLKSGTLEEKEI
                     AQKVVINFEKENDFLDVYKATFWVNPQLRSPMGSNAAAFATKEAAEKANSTKEGTLHT
                     WDELYKNQQ"
     gene            complement(7455..8015)
                     /locus_tag="PK977_01950"
     CDS             complement(7455..8015)
                     /locus_tag="PK977_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF16894.1"
                     /translation="MKKLILLLPLALIIFTSACNNEKKETADTEDTVTTGGQEAVKDD
                     VSQKDIVKVAVGSPDHTTLVAALKQAEYVDDISNAGPFTVFAPTNAAFDKLPAGTVDG
                     LMKDDKKADLQNILEYHVALGVFKENMLQDGQQIGQANGDNITISKSADGKIKINGKA
                     NIVAAIPASNGLVYVIDEVLLPPAKK"
     gene            complement(8078..>9495)
                     /gene="nosZ"
                     /locus_tag="PK977_01955"
     CDS             complement(8078..>9495)
                     /gene="nosZ"
                     /locus_tag="PK977_01955"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRF16895.1"
                     /translation="NFKGTVSFIGVDKTSGKMNIAFQILTPGVNYDLARAGKGKSAGW
                     MFFSCYNSEKANTLLEVNASQKDKDFIMAVNWKKAEEYAKAGKGVKKAVKYAHNVYDE
                     STHMATSTMENEVLVLDPKDCPDMVYYMPCPKSPHGCDTDPTGEYIVGSGKLAAVIPV
                     FSFTKIQDAIAKKNFDGDYDGIPVLKYEAVLHGEVQKPGLGPLHTEFDANGNAYTSFF
                     VSSEIVKWNIKDLKVIDRVPTYYSIGHLCVPGGPTKKPHGKYVIAYNKITKDRYLPTG
                     PELTQSAQLYDISGEKMKLLLDFPTIGEPHYAEAIPAELIMKNSTKIYKIEENKNPYA
                     ALGDNNARVERKGNEVHVYMTSIRSHFTPDNIEGVKLGDVVYFHVTNLEQDWDVPHGF
                     AVKGANNAELLIMPGETQTLAWKPEKVGIYPMYCTDFCSALHQEMQGYVRVSPAGSNV
                     PLTFSTGKTAPAPTKDTTQKK"
     assembly_gap    9496..9547
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9548..10096)
                     /locus_tag="PK977_01960"
     CDS             complement(<9548..10096)
                     /locus_tag="PK977_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HRF16896.1"
                     /translation="MKHKFLNTTMPLLLLTAGLVFMQGCKPKGSQSAVSGNAASKVYV
                     APGKYDEFYNFVSGGFSGQMSVYGLPSGRLFRVIPVFSVDPEKGWGYSEESKPMLNTS
                     HGFVPWDDLHHIALSVTNGEHDGRWAFGNANNTPRIARIDLKTFRTAEIIEIPNSAGN
                     HSSPFITENSEYVVAGTRFSVPI"
     gene            complement(10161..10631)
                     /locus_tag="PK977_01965"
     CDS             complement(10161..10631)
                     /locus_tag="PK977_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF16897.1"
                     /translation="MKSIKLSALVLIAGGAFLFACNEGVKKDEKPVDVQDLTKNQPEV
                     HGSELKEGDITLNTPLEQAMVSAGKSTYELKCQSCHRLTEEKLVGPGWKDVTKKRKPL
                     WIMNMITNVDMMLETDAEAQKLLEQCMVRMPNQNITKDQAREVLEFMRSNDGEK"
     gene            10818..11258
                     /locus_tag="PK977_01970"
     CDS             10818..11258
                     /locus_tag="PK977_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRF16898.1"
                     /translation="MVCSKSFGYALRGVLYVALMSDQKPRVQVEEIAEKLSVPKHYLG
                     KIMKQVVKEGILSSTKGPYGGFSLNEKTLSSPLLSILRITDGFEQFNTCVLRLRKCNS
                     SRPCPLHDQIEANRKAWMQIFSKTSIGDLLNKDEVSFLEGLATI"
     assembly_gap    11584..11594
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11595..12138
                     /locus_tag="PK977_01975"
     CDS             <11595..12138
                     /locus_tag="PK977_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRF16899.1"
                     /translation="NSLPFNDWHIDFLADYIVNVHHHYLRTQLPEIREQLRKFSEEHV
                     KKYPFLQEVEKIFLQLYREMIPHLKQEEEIIFPYIRQIAHAYESSESYASLLVRTLRK
                     PVEEVMNHEHASVVRSVHRIRELTNDYTPPPNACVSHRVTFSKLRELDNDLMQHMHLE
                     NNILFPRAIEMEKILLNTKD"
     gene            12222..>12434
                     /locus_tag="PK977_01980"
     CDS             12222..>12434
                     /locus_tag="PK977_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF16900.1"
                     /translation="MTSLFENATEGIILVNQRGEIVLANPSAGRMFGYEPEDLIGQQI
                     EILIPERFNTYHHHHRDGFYQKPSNRV"
     assembly_gap    12435..12449
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12450..>13421
                     /locus_tag="PK977_01985"
     CDS             <12450..>13421
                     /locus_tag="PK977_01985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRF16901.1"
                     /translation="DLYGKKKDGTDLPVEVSLSHYKKEDEIFVIAFIVDITKRKEIES
                     SMLQQKRELEKITDKIRQLNASLEVKVEERTLILKEALQKLEQSQLELSEALDKERQL
                     NEIKSRFVSMASHEFRTPLSTVLSSASLLAKYKAGEEQEKRDKHIQRIKNSVNHLNDI
                     LEDFLSLGKLNEGKVDKVAGQVSLKEVILDTMDEMRGILKKDQFFDFQCDSDCNACTD
                     KKLFKNILINLISNASKFSDEGKKILIRSKVEDDNVIVSVTDEGIGISDEDQQHLFSS
                     FFRGANATNIQGTGLGLHIVKSYIDLLGGDVGLKSTLNRGTTITFTI"
     assembly_gap    13422..13442
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13443..14547
                     /locus_tag="PK977_01990"
     CDS             <13443..14547
                     /locus_tag="PK977_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF16902.1"
                     /translation="RNGITSKGKSHTKNQRMKSILVIDDNPDIRENTAEILELAGYKT
                     LTAENGKKGVELAQKEKPDVIVCDIMMPELDGYGVLHLLRKNPDTEQIPFLFLTAKTE
                     RSDFRKGMEMGADDYITKPFEDIELLNAIETRLRKAAIMDNKYAASHHGINQFIKDVK
                     NSGLMSQLADQYDVEVYTKKQTLYQEGKRPRFLYYLVKGKVKGFKTHEDGKEYITDLF
                     SDGDFIGYPALIEDKNYDDSAVILEDAEIMQIPREDFLQMIYGNIDIAAKFIRIITQN
                     VKEKEDRLLNLAYSSLRKRVAKALVDIHGKFNAENPNKPLEISREDFAHYVGTATESL
                     IRTLSDFKSEKLIEIKDGKISIINLDKLKNLLF"
     gene            complement(14606..15058)
                     /locus_tag="PK977_01995"
     CDS             complement(14606..15058)
                     /locus_tag="PK977_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF16903.1"
                     /translation="METKAVEKPRTSWPTLFDKGWMDKFFNAPLDEFFNFGKVMNVPA
                     VNVTETDTEFRLAIAAPGLEKKDFKVEAFDDMLTISAEHEREEKEEKNGRYNRREYNY
                     SSWSRSFSLPENCDYGKINAEYKNGELKIMIPKMEVKEPKKVKNISVN"
     gene            complement(15198..15638)
                     /locus_tag="PK977_02000"
     CDS             complement(15198..15638)
                     /locus_tag="PK977_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16904.1"
                     /translation="MFTNSVNMELRIAPERLISEIQKDFNSAFPFLKIEFFNNKSFSR
                     SDFSVKQIIPHNRKLGDSNLSLKDGIIEIEDDMKVNELEKLFKDKFNLAVQVFRKSGN
                     LWLETTMTDSWTLQQQNNHGREITNGKDKELPPDDYDINRDAFS"
     gene            complement(15816..16199)
                     /locus_tag="PK977_02005"
     CDS             complement(15816..16199)
                     /locus_tag="PK977_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRF16905.1"
                     /translation="MSSKEKLRLDKYLWSIRLFKTRTAAAAACDTGKVKYEGVQAKAA
                     KNVNIGDEYEIKTEAKRWRIKVTGLLHKRVAYSEAINYYIDITPEEELQRLQFQAASF
                     HTGKRLSKVGRPTKKQRRDLDEFFE"
     assembly_gap    16360..16455
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16456..>16814)
                     /locus_tag="PK977_02010"
     CDS             complement(<16456..>16814)
                     /locus_tag="PK977_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HRF16906.1"
                     /translation="LGVKELWGEKGYTTYERTGIRPTLELNGIWGGYTGEGAKTVLPS
                     KAYAKISARLVPNQSSHKMTEVLLNYFKSVAPPSVEVKAELHHGGEPYLTPIDSKGYK
                     AASKALETTFGKTPIPV"
     assembly_gap    16815..16855
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16856..17660)
                     /locus_tag="PK977_02015"
     CDS             complement(<16856..17660)
                     /locus_tag="PK977_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF16907.1"
                     /translation="MSQTWKEYQEKNKDRFLNEMLDLLRIPSVSAKSEHKDDMLKCAE
                     AVKKSLLAAGCDKAEVMPTAGHPVVYGEKIIDSSKPTVLVYGHYDVQPPEPLELWHSG
                     PFEPVIKDGKIFARGSADDKGQFYMHVKALEIMTQTNSMNTNIKFLIEGEEEVGSPNL
                     GSFVAAHKDLLKCDVILISDSALLSMENPSLDVGMRGLSYIQVEVTGANRDLHSGTYG
                     GAVANPITILAKMIASCHDENNHITIPGFYDDVLVASKEERDMLNKAPYN"
     gene            17797..18369
                     /locus_tag="PK977_02020"
     CDS             17797..18369
                     /locus_tag="PK977_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16908.1"
                     /translation="MKAVRSIITVSLVLFSLSAMADAGFSIRRRTAPSQITFEGTGNL
                     AGYKLLLVQYSLYGEDTLFRNPFINRRDTITDSYKIVIQDGGKRWDESDRYLHFALAK
                     TDSAGTVTDTFTVYMKKWNYHMLITGEKGGKLEYKMKKTKAYFDYTVIADDESGNNGR
                     INRWIFILCSLAGLVLLILLFLKRKTIKPA"
     gene            18710..18865
                     /locus_tag="PK977_02025"
     CDS             18710..18865
                     /locus_tag="PK977_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRF16909.1"
                     /translation="MLRFFLDMPPEPSFTERAKNNTWLIMIALAAFVALVAFFIWKQR
                     KNKQDRK"
     gene            18862..19176
                     /locus_tag="PK977_02030"
     CDS             18862..19176
                     /locus_tag="PK977_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16910.1"
                     /translation="MNPWLFFVLTLVIEYPVILFFYKKQWKELILPFLLLNLFTWPLL
                     HFFLLTTDISLPVLELGVVIAEMTGYKFLMKSCWKKSFAAAFAANGVSYGAGMLINNY
                     FL"
     gene            19173..>19382
                     /locus_tag="PK977_02035"
     CDS             19173..>19382
                     /locus_tag="PK977_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16911.1"
                     /translation="MNLTHNRNLQPAYWGLLHGINDLAAGFLLAAYTLTHGYSDSFLF
                     ISIYAILGFGGQLPVGFWLDKQKDIS"
     assembly_gap    19383..19460
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19471..>19823
                     /locus_tag="PK977_02040"
     CDS             19471..>19823
                     /locus_tag="PK977_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16912.1"
                     /translation="MASAFVHVTGGTICLQVHENKSGPLGLFTAPGVLGLTIGSLLGE
                     AGYVLPVLLILATAFVTIAIFRQSLPVYQTPEIKESELDSHDLVMLLLLLFMCFRSFL
                     FDVVNYVAENYEAGL"
     assembly_gap    19824..19889
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19890..20226
                     /locus_tag="PK977_02045"
     CDS             <19890..20226
                     /locus_tag="PK977_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16913.1"
                     /translation="FIYGTLVAALILFQFGKTNIYMLCAGIALLQSSVPLTLLLMSRS
                     LSHHPAVATAFSLGIPVALAGLPLYLVSDKRIIHDGFEQPVLAILFFIVLFAVWILLE
                     IKLIKQKIL"
     gene            20297..22045
                     /locus_tag="PK977_02050"
     CDS             20297..22045
                     /locus_tag="PK977_02050"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF16914.1"
                     /translation="MRILFSLLITVYCLTTQAQLGDPFMDANGKWGYKLKEGKIFVAA
                     TYDLALPWNSAGLAAVNIGRKDTVVKGVKKTIPGKWGLLHYTSGLVIPAEFDAIIPFN
                     DDLFLFNGGCTVDANGFMTGGVWGIMDRKKRQLVLDPNYDFIGAMDVQNGVYAVYKGG
                     KYLYNGFVSMPDKNSKGKWALVNGKGMPFTSYAFHAINSVNYAGFLVQDAATRLFGIY
                     SRTGQKTTDCIYDTIYQFHWKDSIAAARKNGKWGFIRYDGREIIPFTYEQIADTDFDK
                     TFIIATRGGTTYVVDKQGREMLDPPVSIDQQFSNALRAANNSKERGAALIAYDDALKA
                     AGYSAGHCSYLVGQKMQQVVDIDFHGVFEYFMKQKVPANGFVGALSILKAEHRSAIKA
                     IAQYIIDEYLAREKGLAPPAWPAGVPKAGYGWGKTMSSDRVVPVTTAPAKPAKTKKEL
                     EEEFLGKRYLVDVAITDGPLYGPRSKAMLAFPKRVDSENNILVYFQYNLPGSNQNWAF
                     YTADELRQLRTPKCNYVPCSYCGGSGVQTSKYRHTNDYSYTLGQKHIYTTTTTSDCNK
                     CCGGKVVVKPCVECEW"
     gene            complement(22137..23303)
                     /gene="dinB"
                     /locus_tag="PK977_02055"
     CDS             complement(22137..23303)
                     /gene="dinB"
                     /locus_tag="PK977_02055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRF16915.1"
                     /translation="MSVIQHHIAHFDLDSFFVSVEILNNPSLKGKPVLIGGYERGVVS
                     TCSYEARKYGIHSAMPMKKALQLCPHAIVTHASSGQYSKYSRWVTDIIAAKEPLFEKA
                     RVDEFYVDLTGMDKYFGAARFAQELRRLITKETGLPISGGLSSARFISKMATNEAKPN
                     GFLQIPHGKELEFLWPLGIEKINGVGKQTEQHLKSFGLYTIEDIAKTPVEIMERIAGK
                     WGEALWHKAHGRGSTEIATDWEQKSMSHENTFDTDYTDLDFLHKELVRLTEKTAYALR
                     EDEKMTGCVTVKIRYSDFETTSRQETVDYTALDDMLIAKVKDLFTKSYQKGRPVRLLG
                     VRFSHMIPITMQMSLFENNIEKLQLFKAVDDIKDRFGTRLLTKAVIANKKKESD"
     gene            complement(23322..23840)
                     /gene="rimM"
                     /locus_tag="PK977_02060"
     CDS             complement(23322..23840)
                     /gene="rimM"
                     /locus_tag="PK977_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611106.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRF16916.1"
                     /translation="MTEYFKIGKLVAVHGLKGELVLKHELGKKTSLKGLAAIFIEEKK
                     NAFLPWFIESAKIKSEDEIYIKLEGVNVRESALKLTQKQVWLPEPDFKKFAARSAPAS
                     LLGYTIINNKESLGEILELIEQPHQLLCRLEIKGKEVLIPLHEETLRKVNHKNKEVVV
                     NLPDGLLEIYLQ"
     gene            complement(23931..>24142)
                     /locus_tag="PK977_02065"
     CDS             complement(23931..>24142)
                     /locus_tag="PK977_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16917.1"
                     /translation="LGCWRKAYLERLGLFDEATAMEKFTKWSAEHEAQVKRRQEDSYR
                     QKRARRAAPGRRPERKAEGEGGSEG"
BASE COUNT         6728 a         4774 c         4909 g         7151 t
ORIGIN      
        1 ttcgatggtt tgtgcattac gcaggtaaaa gcggttcttc ccgagtattc tcagaatatt
       61 tccattggta aaagtattgg atagtaacaa ggcacaggga caagcaatga tgaatatggc
      121 ggttactgca ggccatatcc tcgaaggatc attgatttgc cagtaaatag cagtgcccag
      181 ggcaatggta agtactacat atataaagta acggctgagc aggtgaacaa atgaaactga
      241 tttcgtttct tcctgccgga actcatccct gttccagagt ttagtcagat agctttgtgc
      301 tacttctttt actaccaata tttcaatatt gccctcagtc tgtttgccac ctgcataaac
      361 cagctctccc acttctttta aaacaggtaa cgattcgcct gtaacaaaac tataatcaat
      421 aaatgctttt ccttttgtca atataccatc cgcaggaatg agttcttcat tatgtatcag
      481 tagcgtatca ccaggtttta tttccggtaa tgtcgttgga acttccttat tgtctttaag
      541 tactgaaata gcaataggga aataagatgt gtaatctctt tcgaacgaaa gctgccggta
      601 ggtcttatcc tgtacaatcc tgccagccag cataaagaaa acgatccctg tcattgaatc
      661 gaaatatcct ccaccagttc cacttagtac ttcccaggca cttcttacaa aggtgatgat
      721 aatggctaat gcaataggtg catctatatt caggaattta tgttttaggc ttctccagga
      781 tgactgataa aaaggcagtg ccgaataaag aagaacagga atggcgagcc cgaaattcag
      841 ccagcggaat atttctctga gcccggtttc actggcatct atgcccaggt actccggaaa
      901 actcatcagc ataatattcc caaaagcaaa tccagcaata cctaattgat acaccatgct
      961 cttgttaatt gccggacgct tttctttcaa atcgttcagg ctgatatagg gctcatagcc
     1021 gatgctggtc agtacttcgg ctacttttct caagctggta tttgctgtga gtaaaacgat
     1081 ttcaacttct ttgcgggtaa aattaacctt gctggaaact acacccggat caagccgatg
     1141 caaattctcc agcaaataca aacaggaact gcaatgcatt tgcgggaggt agaaagtaac
     1201 atgtatttgt ttgttgtctt taaaactaac cagctggagc tgaatttttt catcgtcaag
     1261 gaaagcaaat ttgtcctttc gtactttaat ccgctgactg agaccaggat tttcattcag
     1321 tgagtaataa tcacagagcg aatgctcatt taaaatctgg aaaaccattt tacagccttc
     1381 gcagcaaaaa tgcttttctt ccagctgtat cttatcgttt ggacatgttt caccacagtg
     1441 ataacagcta attttttctt tggtaacacg atcaggtgaa agcatatctt tccaaaataa
     1501 ggcgctaaag tattcctctg ttaaaacact gtatttgagc aacctcaaaa agtaaactga
     1561 tttttatcag gtcctttctt cggaaacttt atgaagagag tggatggtaa ccagctcttc
     1621 ttcaactgat tgcttctttt gactatagat ggccagtgtc ttataaaccg taaaaattaa
     1681 aagaaagaga ccaacgaaaa tagcgatacc gctataagca aaaagtttaa accagggctg
     1741 gcttttttgc attatagaag caaatacttc attgttgagc ttagcactaa tcttcagcag
     1801 accgctacct agcagagagc tccagaacag tagaagcgaa acatttgtga tgaggatgcc
     1861 ccagccgatt gtaggtttgc ttctttcaaa tactggttta ttttcgtttt gaataatata
     1921 gaatacagat gcaaaaagta acatagtatt gatgccaatt gtagtgccca tagcatgggc
     1981 tactgtaata tgagtaccgn nnnnnnnnnn nnnnnnnnnn ccaggaagga acggagatga
     2041 caatagccag tgatagattc agaaatatcc agatatcggc ataagacagc aggcgataag
     2101 gtatgttatg ataattttta cgagcctgtg tcatggtttt tctccatttc ataatgatat
     2161 atccaaggat aagcaactcc gtcatactta tcacatatgc tactgtctta acccagggtg
     2221 ctgctggcac aatataagta tgatggcccc agttgaacat caggttggtc agtccgagaa
     2281 aataaaaaaa gaaagcaaga ggtgagcggt tggctttgtt gtcaccactc attttttcca
     2341 tcacatacat gccggttcca tacaccagca tattccaact gcccaccata gagcccaggg
     2401 ctttccactg taccgttaca tctcttacga tattggtatg aaaataatca agcaaccata
     2461 aataagactc agacagtgta ataaagaaga aaagtatgcc cgttgaccag ctccagatat
     2521 aaacaggtgc gtttttataa tcaggcttca gggtaaaaaa gaagttgatg gcaaagggta
     2581 cccaagacag caagattaca gctcccagca acggtggaaa ttcgaggtat tcccttccgc
     2641 caaaatagcc aaggaaaaaa caagcaacta taaaaaaaga aactaatgcg tgaagataaa
     2701 aatgtatccg ggcaccttgc agccagtaaa ttggtctgtt ggcgattctt ggaaggtaat
     2761 aatacaagcc ggcttgtgca gctgtgaata tccagctgat tacaagatat acatgcagcg
     2821 gccttgtttt ttgaaaggcc agttgctctt tcaaaaatgg gggcagaatg tattgtatcc
     2881 ctccaataat acccagcagc aaaccaaaaa aaagataact gatagaaagc aacagaaatg
     2941 cctttgcggt tttgtcagaa gcgtattgac tggaattggt aagcataact gatcaaggca
     3001 tggatttata ggtgactgca gttgagtcaa catattttaa atagctgata atagcatcta
     3061 tttcatctgc tttgaaatga aaattgggca ttctaggtcc accggcctgc aggaatgctt
     3121 tcatatatat ttctccccgt tgcggatcac tgtaggcagt tgtcagttct ggacccagat
     3181 atccccnnnn nnnnnnnnnn nnnatccccc caatccatat agctggtggc aggcaacgca
     3241 gttatattgc tcgtacaggt atttgccttg agatactttt tctgcttgtg gtcctgttaa
     3301 ctgtatccca ctttctgttc cttttgaata aataacagta ctataaatag catagctggt
     3361 aaagagaatg agaaagacag ctattttgat catcagcact ttgtttgaag gggcgaatgt
     3421 attttccggc ttttcagacc atgctgattt tactcagtta ctgggctgat aattctcaat
     3481 tattctactt tttttatgga ctataaagct atactnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgtata gccagccaaa
     3601 gcggtaatac agtccacagc agcattatgc ctatggtaaa cagaagaccg gtgctgctag
     3661 taaagaaatc tttatataat gcgctggtga cacccattaa tgcactgata tccattttca
     3721 gtgtgacaaa aattcttcca acatcaattg ggttcagcga tattagcggt aatgtgattt
     3781 tttcgagggg ataatcactc aggttaaaga tcatcagtaa tataagcccg tcaaaaatca
     3841 gtgtaaaata gaaccacaga aggagagctg cgccaatgcc tcttgccttg tctcttgacc
     3901 tgactgatgc aaaaaatgca atggcaacaa aaacaaccgt tagggccatc ccggttaaaa
     3961 gcagggcaaa gccggtttca tccggtgcat aaagtaagat gggtaaacca acgccgacca
     4021 ggaaggcact aagtaaagag acactgacac cggcaaactc acttattatg atacgggttc
     4081 ggctcattgg ctgcgatgac atcagctcaa taaattcgta agaattataa taatgaatgg
     4141 tggtaaatac aaggctcacc agcggtaaaa cgatcaatac aatattcagc agactcagca
     4201 tggccttgct gctgttttct tccatattga acatgctcaa cgaaacaatc agtagaaaca
     4261 gggtataggc taaaacgatc ttacttctca gtatatcata cagcacatat ttggatacct
     4321 tcatcatagc tgtgtggttt aatagatttt ctcgtctttt tttacttcca gtatcttgtc
     4381 aatccactca ttggtgagtt tttctccttt catgatcctt gcaatggctt tgccaagtct
     4441 aagttccccg gtttcttcct gtagtgtctg catgtcttta aagaattgca gtttgccttc
     4501 ctgcaggtac attacatgtg tggtcagttc gtccaggtcg cttaatatat gagaagtgat
     4561 caggatcagc ttattctttt tcttttctgc aatgatcttg tctttcaaaa gttcggaaga
     4621 aagcggatca agaccagcgg taggttcatc aagaattaat atagcaggat caaacaaaaa
     4681 agccagtgcg gcgctaacct tttgacgggt acctccgctc aatgttcgca tgggcttatc
     4741 gtatattttt tccagctcaa attgcctgat cagcgtttca tcctgcttat ctgctgctac
     4801 tcctcttata ttttcaatca tgcggaagag ctgcccgatc ctcatattgt caggatagcg
     4861 gccaatctgc ggcatatagc caatattagc ccggtaggaa tgatcaccac tgattacatg
     4921 accatcaacc gtaatcttgc ctttatcggg ccgtaccata cccagtatag atttaataag
     4981 ggtagttttt ccggagccat tgggcccgat aagtgaaata acctgtccct tttccatatt
     5041 ggcattaatg tcttccaata cctggagctt ccggaaccgc ttgctgatat gttcaatact
     5101 gatcataact tcagtgattt catcattggt tttttgtcta caagattttc aggggtaagg
     5161 cttggtatgg ctttttctgc tttgtcaagc agtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnggg aacatcaccc acgccgtccc tgttaatatc atatccttca tatttatccc
     5341 aatagttgtt gctgaattca tttagtgaca acgacccgtt ggtgccaaca tcaaagctgt
     5401 tgccggtaaa attattacgt tgaaaactat tattctcaca actggcctgt actttaatag
     5461 cccagccatt ttctttaaat ttatttcgct caattgtcat acggctggta ccctccatat
     5521 gtatgccaac tgtgttttta taaaaagtat tgtgactaat atagctgtca gaaatatctt
     5581 tcaatagtat tccataggct gatggccccc agttttggtc aaaatgattg ttttccattt
     5641 ttaccttttt tgaatacatc acggccacac cggcaccatt atctttaaaa gtgttattca
     5701 ggtaggcatc atcgttggaa aacatgaaat gtaagccgta tcggatattt ttttcactat
     5761 gattatttcg aatagtagag ttggtcacaa attcaaaata gataccgtcc ctgtgtccct
     5821 gaatagtatt gttttcaatt aatgcgtatc tcgatttcca aagatgaatg ccatttccgg
     5881 tcaattgttc agatgtcggg gtgccggtaa tgttattgtt cttaatagtg gcataactgg
     5941 tatttgacac atgtattgcg aagtaagcgt tggttatttt atttgcggca atggtgactt
     6001 ttgaggcgtc caccagtttt atggaagcat aatcattcag cgcagaataa cctgagttac
     6061 gaaaagcaag tcctgtaacg gtgatgtttt gtccacttat aagaagaatc tcgtatttgt
     6121 tttctccatc cagtattgga taattttgac caatgatggt taacgatttg gttaattgca
     6181 aagctccttc tttatagacc ccgggcagta ctgttatgct gtcaaaatct ttggctatct
     6241 caatggcttt ccggatagat gatactggtt ggttctttcc tacgatgata ttacggctaa
     6301 atccgtttgc cgacaaaaag agtaataata tcagtgcgag gcgatgcatt ttattgctga
     6361 tttttataca attcatccca ggtatgcaat gtaccttctt ttgtgctatt ggctttttca
     6421 gcagcttcct ttgttgcaaa agcagcagca ttacttccca tcgggcttct tagctgtgga
     6481 tttacccaaa aggttgcttt atatacatca agaaaatcat tttctttttc aaagttgata
     6541 acgacttttt gagcaatttc tttttcctcc agggtaccag acttaagaaa attgatcatg
     6601 catacaaggt catcaaattt ataaacctta cttttcttag taaccacttc acaaccgtat
     6661 ttgggatcca taatgcccat tttacagaag tggcaattat cctgtccata tttaaaaggt
     6721 tcaggttttg cagagcagga gctaaagaac aggcaaagaa taataaccgg cgccgcaaaa
     6781 gtggtggttt taagtttttt ggaggtctta cgatttttat accattctgc aaaccaaacg
     6841 ccaaatgcaa gacagcctgc tccaattaca atccatccac ccacatcagg gaaagagtaa
     6901 gaatcaaagt ttagcagccg tttatgcccg attagtggtg gctgatagga catgcccggc
     6961 acctggatag gtgcttttgg atcaagattg tgtccgtatt tgtaacccca ttggtaaaaa
     7021 tcccacatag ccaatatgcc acccagaacg gtcagaccta tataagcaag caggaacttg
     7081 cggttgccgg taatggcgac tatcataccc agcagcataa aaaaaccaac tacgtagggc
     7141 aggtatttaa attcagggaa catgttaacc gttatgtgct ccattccgat ataatggttt
     7201 agaccgttta taatatctac atcaccagta aggccattaa tccagatgtt catagtcaga
     7261 ccttccggat attgcggggc aaaaagatct atacgccagg cgggcagaaa gaaaacagca
     7321 atcaatgcac ctgatgcaaa tgctactaaa attctggaag caacacttaa tttgttattc
     7381 ataacccttt ttttaatgac ttccggggca ttacaccccg gaagcttcaa tttttaaaac
     7441 ttcaacaaga aaagctattt tttagccggg ggaagcaata cctcatcaat tacatatacc
     7501 agcccgttac tggcgggtat ggcagctaca atatttgctt taccatttat ttttatttta
     7561 ccatctgcag atttgctgat agtaatatta tctccgttgg cctggcctat ctgctgacca
     7621 tcctgcagca tattttcttt aaatacgccc agcgcaacat ggtattcaag aatgttctgc
     7681 aaatcggctt ttttatcatc tttcattaga ccatcaacag ttcctgctgg tagtttatca
     7741 aaagcagcat tagttggtgc aaacacggta aaaggacctg cgtttgaaat atcgtcaaca
     7801 tattcagcct gttttagggc agcaaccaat gtggtatgat cgggtgagcc aacagctact
     7861 tttacaatat ctttttgtga tacatcgtcc ttcacagctt cttgtccgcc ggtagttact
     7921 gtatcttcag tatcagccgt ttcttttttt tcattattac aggcacttgt aaaaatgatg
     7981 agtgccagcg gcagcagtaa gattagtttt ttcataaact tatatttaag tatgaaatgg
     8041 ataagagcaa ccgcttttgc ggttgctctt cagttgatta tttcttttga gtggtatcct
     8101 tggtaggagc aggtgctgtt ttacccgtac taaacgtaag aggtacatta gatcctgcgg
     8161 gacttactct gacatatccc tgcatttcct ggtgaagggc agaacagaag tctgtacaat
     8221 acatggggta gatacctact ttttcaggtt tccatgccaa tgtctgagtt tcgccgggca
     8281 taatgagcag ttcagcattg ttggcgcctt tcacagcaaa gccatggggc acatcccagt
     8341 cttgttccag gttggttaca tggaagtaaa caacgtctcc aagttttaca ccttcaatat
     8401 tatctggtgt gaagtgagaa cgaatagaag tcatgtaaac atgcacttcg tttccttttc
     8461 tttctactct tgcattatta tcacccaatg cggcataagg gttcttgttt tcttcgatct
     8521 tatagatctt ggtagaattc ttcatgatca gttcagcagg aattgcttct gcgtagtgag
     8581 gctcaccaat ggtggggaag tcaagcaaca gcttcatttt ttcaccgctg atgtcataca
     8641 actgtgcact ttgcgtcaat tcagggcctg ttggcaggta acggtctttt gtaatcttgt
     8701 tgtaagcaat tacatatttg ccatgtggct ttttagtagg gccaccgggc acacaaaggt
     8761 gaccaatcga ataataagta ggcacacggt ctattacttt caggtcttta atattccatt
     8821 ttactatctc ggatgataca aagaaagagg tataggcatt accattggca tcaaattctg
     8881 tatgcaaagg gcctaatccg ggcttttgta cttcaccatg taaaacagct tcgtatttca
     8941 aaacagggat accgtcatag tcgccatcaa aatttttctt agcaatcgca tcctggatct
     9001 tagtaaatga gaatacagga ataacagctg ccagtttacc acttcctaca atgtattcac
     9061 cagtgggatc ggtatcacaa ccgtgtggtg atttaggaca aggcatgtaa taaaccatat
     9121 cggggcaatc tttgggatca agtaccagca cttcgttctc catagttgaa gtagccatat
     9181 gtgttgactc gtcatacaca ttatgtgcat acttcactgc ttttttaact ccttttccgg
     9241 cttttgcata ttcttctgct tttttccagt ttactgccat gataaaatct ttgtctttct
     9301 gcgaagcatt tacttccagc agtgtattgg ccttttcaga attataacag ctgaagaaca
     9361 tccagccggc agattttccc ttaccggcac gggcaaggtc atagtttaca ccaggtgtaa
     9421 ggatttggaa agctatattc attttgccgg aagttttgtc aacgccgata aaacttacag
     9481 ttcccttaaa gttttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnaat aggcacacta aaacgggtcc cggctacaac atactctgaa ttttcagtga
     9601 tgaaaggaga agagtggtta ccagcactgt tggggatttc aataatttca gctgttctga
     9661 atgtcttcag gtcaatgcgg gcaatacggg gagtattatt agcattaccg aaagcccagc
     9721 ggccatcatg ctcaccatta gttacagaaa gggcaatgtg gtggagatca tcccagggaa
     9781 cgaatccatg agaagtgttc agcatgggct tgctttcttc actataaccc cagccttttt
     9841 caggatcaac tgagaaaacg ggtataacac ggaacagacg ccctgatggt agaccataca
     9901 cagacatctg tccgctgaaa cctccggaaa caaagttgta gaattcgtca tacttgccag
     9961 gggcaacgta aaccttagag gcagcattac cgcttacggc agattgcgat cctttgggtt
    10021 tgcagccctg catgaaaacc agtcctgccg ttaataacaa gagtggcatc gttgtgttta
    10081 gaaatttgtg tttcataata tgtatttagc agttttgaaa agagattcct ttgtttccgt
    10141 tggttattgt tttgggcttt ttacttttcg ccatcattgc tgcgcataaa ttccagtact
    10201 tccctggcct gatctttcgt tatattctgg ttgggcattc tcaccataca ctgttccagt
    10261 agtttttgtg cttcagcatc tgtttcaagc atcatatcaa cattggtaat catattcatt
    10321 atccaaagag gttttctttt tttagttaca tccttccagc ctggtcctac cagtttttct
    10381 tcagtcagcc tatgacaaga ctggcatttc aattcgtaag tacttttgcc agcgctgacc
    10441 atagcctgtt ctaaaggagt atttaaagta atatctcctt cctttaattc agagccatgt
    10501 acttcaggct ggttcttggt caaatcttga acgtcaactg gcttttcatc tttttttacg
    10561 ccttcgttac aggcaaataa gaaagcgccg ccggcgatca atacaagggc tgaaagtttg
    10621 attgatttca tgtttaattt ttttgtgttt aggtgatata atgtctttgt taaagttact
    10681 tgatttttaa aaaagataca gatgtcctta atggggtcaa aagtatttgg gagccaataa
    10741 gtccatcatg acaaccatca aataattagc tgatatttat cacccttaaa aagatcaacc
    10801 tggtcttttt ttgcaaaatg gtgtgttcta agtcttttgg ttatgcttta cgaggagtgc
    10861 tttacgttgc actgatgagt gatcagaagc ccagggtaca ggtagaggaa attgccgaaa
    10921 agctatctgt tcccaagcat tacctgggta aaatcatgaa gcaggtggtg aaggagggca
    10981 ttctttcgtc caccaagggg ccctatggtg gattttcttt gaatgaaaaa acgctatcga
    11041 gtccactgct gagcatcctt agaatcaccg atgggtttga gcagtttaat acttgtgtgc
    11101 ttagattaag aaaatgtaat tccagtcgcc catgcccgct tcatgaccag attgaagcta
    11161 acaggaaggc atggatgcag atattttcta aaaccagtat aggagacttg ttgaacaaag
    11221 atgaagtcag tttcttagaa gggctggcta caatttaaag gcacagcgac gatataaaat
    11281 cattcccagg cctgatttaa gtcaggtcac cagcatatta catattctaa tttgccgtca
    11341 aatttttctt tttaaaaaag aagtgagagt actatgtttc tgaagcctta tcggatagaa
    11401 ccaacttcat tcgtgacaga tatagtaacg gctgattacc gtacggcaga ggtatttcgt
    11461 aaatatgata tcgaatattg ctgtggtgga aaatggccac tggaaatggt atgccatgca
    11521 aagggtattg atatcaaaga aattaccagg gaactggaac agtctattcg ttccgttcag
    11581 gtgnnnnnnn nnnntaattc attgcccttt aatgactggc atattgattt tttagctgac
    11641 tatattgtaa atgttcatca ccattatctg cgcacacaac ttccggaaat aagagaacag
    11701 cttagaaaat tttctgaaga gcatgtaaag aaatatcctt ttttacagga agtagaaaag
    11761 atattcctac aactttatag agaaatgatt ccccatctta aacaagaaga agagatcatt
    11821 tttccctata tccgccagat agcccacgcc tatgaaagca gtgaatctta tgccagtctg
    11881 ttggtaagaa cactacgcaa acctgtggaa gaagtaatga accacgaaca tgcttctgta
    11941 gtgagatcgg tgcaccgaat aagagaactg acaaatgatt ataccccccc gcccaatgcc
    12001 tgcgttagcc atagggtcac tttctctaaa ttaagagagc tggataacga cctgatgcag
    12061 catatgcacc tggagaataa tattctcttt ccaagggcca tagaaatgga aaaaatcctg
    12121 ttgaacacta aggactgatt tgttttacca cattttttct ctcaacctgt tttccttaat
    12181 ttgctgcttt ttaagagctt atgcatcttg acgcagcaca tatgacttct ctttttgaga
    12241 atgctacaga aggaatcatc ctggtaaatc aacgaggaga aattgtattg gccaacccct
    12301 ctgctggccg gatgttcgga tatgagccgg aagatctgat tggacaacaa attgaaatac
    12361 tgatacctga acggttcaac acttatcacc atcatcaccg ggatgggttt tatcaaaagc
    12421 catccaaccg ggtcnnnnnn nnnnnnnnng agacctgtat ggtaaaaaga aagacggaac
    12481 agatttaccg gtagaagtga gcttgagtca ctacaaaaaa gaggatgaga tttttgttat
    12541 agcctttatc gtggatataa caaagagaaa ggaaattgaa agcagtatgc ttcagcaaaa
    12601 aagagagctg gaaaaaatca cagataaaat aagacaactg aatgcgagcc tggaagtgaa
    12661 ggtagaagag cgaactctca tattaaaaga agcattacaa aaacttgaac agtcacaatt
    12721 ggaactgagc gaagccctgg ataaggaaag acaactgaat gagattaaaa gccgctttgt
    12781 ttcaatggct tcccacgaat tccggacacc gttgagcact gttctttcgt ctgcatcatt
    12841 gctggctaaa tacaaagccg gagaggagca ggaaaaaaga gataaacata ttcagaggat
    12901 caagaattca gtaaaccacc tgaacgacat tctggaggac ttcctttctt taggaaaact
    12961 taatgaggga aaagttgata aggtagccgg ccaggtttct ctcaaggaag taatacttga
    13021 tacaatggat gagatgaggg gcattttgaa aaaagatcaa ttttttgatt ttcagtgtga
    13081 ttcagattgc aatgcctgta cggataaaaa gcttttcaaa aatatcctga tcaacctgat
    13141 tagtaatgcc agcaagtttt cggatgaagg aaagaaaatt cttatccgga gcaaagtgga
    13201 agatgataat gtgatagtat ccgtgactga tgaaggaatt ggcatatccg atgaggatca
    13261 gcagcatctt ttttccagct ttttccgggg ggccaacgcc accaatatcc agggtacagg
    13321 gcttggatta cacattgtta agagctatat tgatctgctg ggaggagatg ttggattaaa
    13381 gagtacatta aatagaggaa caacaattac ctttactatt cnnnnnnnnn nnnnnnnnnn
    13441 nnccgaaacg gaataacctc aaaagggaaa agccatacaa aaaaccaacg catgaaatca
    13501 atactggtaa tcgatgacaa ccccgatatc agggaaaata ccgcagaaat attagagttg
    13561 gcagggtata aaaccctgac agctgaaaat ggtaagaagg gtgtagagtt agcccaaaaa
    13621 gaaaaacccg atgtaattgt ttgtgatata atgatgccgg aactagatgg gtatggtgtg
    13681 ctgcacctgc tcagaaagaa cccggataca gagcaaatcc cttttttatt tcttactgct
    13741 aaaacagaac gaagcgattt tcgaaaagga atggaaatgg gcgccgatga ttatataacc
    13801 aagccatttg aggatatcga actgctgaat gctatagaaa cccgtcttcg aaaagcggct
    13861 attatggata ataagtacgc cgcctctcac catggtatca atcagttcat taaagatgta
    13921 aaaaattcgg ggctgatgag ccaactggct gatcagtatg atgtggaagt ttacacaaag
    13981 aagcagacac tttaccagga gggaaaaaga ccaagattcc tttattacct ggtaaaaggc
    14041 aaggtaaagg gatttaaaac tcacgaagac ggcaaggaat atattacgga tttgttcagt
    14101 gacggtgatt ttattggtta tcctgctttg atagaggata aaaactatga tgattctgct
    14161 gttattcttg aagatgcaga gattatgcag ataccaaggg aagattttct gcaaatgatt
    14221 tacggcaata ttgatattgc tgccaagttt atccggatca ttacacagaa cgttaaagaa
    14281 aaagaggata ggttgctgaa ccttgcctat agctctttaa gaaaaagagt ggcaaaggcc
    14341 ttggtggata ttcatggtaa atttaatgca gaaaacccca acaagcccct ggagatttca
    14401 agagaagatt ttgcgcatta tgtaggaacg gctacagaat cacttatccg gacacttagt
    14461 gactttaagt ccgagaaact gattgaaatt aaggatggaa aaatatccat catcaactta
    14521 gataagctta agaacttatt attttaaata aaaccctccg cattgcggag ggtttttctt
    14581 ttcatcaggg tatgtccctg tacgatcagt ttacagagat gtttttaacc tttttgggtt
    14641 cttttacttc catttttggg atcatgatct tcaactctcc gttcttgtac tcagcattga
    14701 tctttccata atcgcagttt tctggtaatg agaaactacg gctccagctg ctgtaattgt
    14761 actctctgcg gttgtagcgt ccgttctttt cttctttttc ttctctttca tgctcggcac
    14821 tgatggtcag catgtcatcg aatgcttcta ctttgaagtc ttttttctca agaccaggag
    14881 cagcaattgc aagcctgaat tcagtgtctg tttctgttac atttacagcc ggaacattca
    14941 tcaccttgcc aaagttgaaa aactcatcga gcggagcgtt gaaaaactta tccatccagc
    15001 ctttgtcaaa caaggttggc caggaagttc tgggtttttc aacagctttt gtttccataa
    15061 aactgagttt taaggtgaac aataattctt atacattaga aagttatttt gacatttaag
    15121 tcagaaacaa tgacaggcgt aagttgctgt actgattttt gtcagcaata caggcgcagg
    15181 taatttaagg agatggctta actaaaagca tccctgttga tatcatagtc gtcaggaggc
    15241 agttctttat cttttccgtt cgttatttcc cttccatgat tattctgttg ctgcagggtc
    15301 cagctgtcgg tcatggttgt ttcaagccat aggttgccag attttctaaa gacctgaaca
    15361 gcaaggttga atttgtcttt aaacaatttt tccagttcat taactttcat atcatcctcg
    15421 atctcaatga taccgtcttt cagtgaaaga ttactgtcgc ccagtttacg gttatgcggg
    15481 atgatctgtt ttacggaaaa gtctgaccgg gaaaaggact tattattaaa gaactcaatt
    15541 ttcaggaatg gaaatgcact gttgaaatcc ttttggattt cactaatcag ccgctcaggt
    15601 gcaattcgca attccatatt cactgaatta gtaaacatag tttaatgaaa taacaaatac
    15661 cggctcagta tgcccgaagc ttccttgttt tattcttaac aacagtattg atgtgggcat
    15721 aaagagaagg ttatttatga cgaagctaac ctcctgctta gcggatgtga atgatgcaaa
    15781 taatacaaca agatgatttt catcactgag gagagtcatt caaaaaactc atcaaggtca
    15841 cgtctttgtt ttttggtagg acgtccaact ttacttaacc gctttccggt atggaaactg
    15901 gccgcctgga attgcagcct ttgcaattct tcttctggag taatatcaat ataatagttg
    15961 atggcttcgc tataggcaac ccttttatgc agcaggccgg taactttaat ccgccatctt
    16021 ttggcttccg tttttatttc atactcatcg ccaatattta cattctttgc agcctttgcc
    16081 tgcactcctt catattttac tttaccggta tcgcaggcgg cagcagcggc tgttcttgtt
    16141 ttaaacagtc ggattgacca gaggtattta tcaagccgta atttttcttt ggaagacata
    16201 ttcaaaaata aaaaacctca tcaaaaagat gatgaggttc taaaagtaaa agaagtttac
    16261 gtttatgatt cagcaaaata cttatggaaa tagggaatag tttcaatgcc cttaaaataa
    16321 ttttcgatat tatatttttc atttgggcta tgaagattgn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnngaaca gggatcgggg ttttcccaaa agtggtttcc agtgctttgg
    16501 aagctgcttt ataacctttg ctgtcgattg gtgtcaggta aggttcacct ccatggtgca
    16561 attcagcttt tacttcaaca gagggaggag caacagattt aaaatagtta agcaatacct
    16621 cagtcatttt atgagaggat tggttaggta ccaaacgggc agaaattttt gcgtaggcct
    16681 ttgaaggcag aaccgtttta gccccttcgc cggtatagcc accccatatg ccatttaatt
    16741 caagagtagg gcggatgccg gttctttcgt aagtggtata gcctttctca ccccatagct
    16801 cttttacacc taatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatta
    16861 tatggggctt tgttcaacat atccctttct tcttttgaag ccaccaaaac atcatcatag
    16921 aaacccggga tggtgatgtg gttattctca tcatgacagg aagcgatcat tttagccagg
    16981 atggtaatcg ggttggccac agcaccacca taggtaccac tgtgcaggtc acggttggca
    17041 ccggtaactt ctacctgtat atagcttagg cctctcattc caacatccag tgaaggattt
    17101 tccatgctga gaagggcact gtcgctgatc agtatcacat cacatttcag caggtcttta
    17161 tgtgctgcta caaaagagcc caggttggga gaacccactt cttcttcgcc ttctatcaga
    17221 aatttgatat tggtgttcat gctgttggtc tgcgtcatta tttccagcgc ctttacatgc
    17281 atataaaact ggcctttgtc atctgcggat cccctcgcaa atattttacc gtcttttata
    17341 acaggttcaa acggcccgct atgccaaagt tcaagtggct ctggtggctg tacatcatag
    17401 tgtccataga caagtactgt tggttttgag gagtcaatta ttttttcacc ataaaccacc
    17461 gggtgaccag ctgtgggcat aacctcagct ttatcgcatc cggctgccag caggcttttt
    17521 ttcactgctt cggcacattt caacatatca tctttatgtt cgcttttggc actgacagag
    17581 ggtattcgca aaaggtcaag catttcgttc aggaaacggt ctttattttt ctcctggtat
    17641 tctttccatg tttgtgacat aaaattagtt tttagggatg acgaagataa gcaggaagag
    17701 cttatttctg cctgaggttt ttgacagccg aacctgatct gtatataccc ggctctgggt
    17761 atgcagggat caatcaaatt atctacattt agcattatga aagccgtacg gtcgataatt
    17821 acagtgtcac tggttctgtt tagcctgtca gctatggctg atgcaggatt ttctatccgc
    17881 aggcgaacgg cacccagcca aattacattt gaaggaaccg gcaatcttgc cggttataaa
    17941 ttgctcttgg tacaatactc tttatatgga gaggatacat tgttcaggaa tccttttatc
    18001 aaccgaagag atacgataac tgacagttac aaaatagtta tacaggatgg gggaaagaga
    18061 tgggatgaat ctgatcggta tttacatttt gcgctggcca aaacagattc ggccggaacc
    18121 gtgacggaca cctttactgt gtacatgaaa aagtggaatt accacatgct tattaccggt
    18181 gaaaagggag gaaagcttga atacaaaatg aagaaaacaa aagcctattt tgattatacg
    18241 gtcattgctg atgatgagtc tgggaataat ggccggatca accgttggat atttattctg
    18301 tgttctttag cagggcttgt gcttttgata ttactattcc tgaaacgaaa aactataaaa
    18361 ccggcttagg ccttatttgc atatgccatt gatcattgca ctttttggtt tttttataat
    18421 taaagggatt ttccaactgc ctttttacct gcggcagtgg aaagcatcgc tgctagtggc
    18481 actactcacc agtttgacag ttgtgccgtt cattaccata ttgtaccacg aaacttcttt
    18541 tgatttcacc atagtctatg caggaatgat attactggat attattgtat taaacttatt
    18601 ggtgctaaac aactggtgga aagcaatacc ggcagcctta attgtcaata cgattgctat
    18661 tctgtacttt ttcattggaa atggttaatc agaattaaaa tttacataca tgctcagatt
    18721 ttttttggat atgccaccag agccatcttt tacagaaaga gctaagaata acacatggtt
    18781 aattatgatt gcattggcgg cttttgttgc tttagtggct ttctttatct ggaagcaacg
    18841 gaaaaataaa caggatagga aatgaatccc tggctatttt ttgtgctgac actagtgata
    18901 gagtacccgg taattctttt cttttataaa aaacagtgga aagaattaat ccttccattt
    18961 ctactgttga acctttttac ctggccgctc ttgcattttt ttctgctgac aaccgatatt
    19021 tcacttcctg tactggaatt gggagtggtt atagcagaaa tgacagggta taaattttta
    19081 atgaagagct gttggaaaaa atcgtttgcc gcagcatttg ctgctaatgg ggtaagttat
    19141 ggcgctggca tgctgattaa taattatttt ttatgaattt aacccataac cggaatttac
    19201 aaccagctta ctggggattg ctgcatggca ttaatgatct tgccgcagga tttctgctgg
    19261 cagcttacac attgacacat ggatatagcg acagtttttt gttcataagc atttatgcga
    19321 ttctgggttt tggtgggcag ttgcctgtcg ggttttggct cgacaagcaa aaggatattt
    19381 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn atgttcagga atggcttcag catttgtaca tgtaaccggc
    19501 ggaacaattt gtcttcaggt gcatgaaaat aaatccggcc cattaggatt atttactgct
    19561 cccggtgtat tgggccttac cattggtagt ttattaggtg aagctggtta tgtgctgccc
    19621 gtattattaa tacttgccac agcttttgta actatagcca ttttcagaca gtcattgcca
    19681 gtatatcaga ctccggagat aaaggaaagc gaattggaca gtcatgattt ggtgatgctg
    19741 ttgttactgc tttttatgtg ctttcgttct tttttgtttg atgtggtaaa ctatgtagct
    19801 gaaaattacg aggccggcct gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tttatatacg gaacacttgt tgcagctttg
    19921 attcttttcc agtttggaaa aactaatatt tatatgttat gcgccggcat tgcattgctg
    19981 caaagttctg tgccgcttac ccttctgctg atgagccgca gtttatcaca tcatcccgca
    20041 gtggccacag ctttcagcct gggaataccc gtagcgctgg cagggttacc gctgtacctg
    20101 gtatcggata agcggataat ccatgacgga tttgaacagc ccgtactggc aattttattt
    20161 tttattgtac tgtttgccgt ctggattctt ttagaaataa aattgatcaa acaaaaaatc
    20221 ttgtgactgg caatactttt ttactaattc accggtatga gaaaccggtg tatgacaaac
    20281 tataagaaaa aaaactatgc ggattctgtt ttcactactt atcacagtat actgtcttac
    20341 aactcaggca caacttggcg atcccttcat ggatgcaaac ggtaaatggg ggtataaact
    20401 aaaggaagga aaaatttttg ttgcggcaac ttacgacctg gctctgcctt ggaacagcgc
    20461 cggtttggct gctgtcaata tcggcagaaa agataccgta gtgaagggag tgaaaaaaac
    20521 aattcccggg aaatggggat tactgcatta cacttctggc ctggtgattc cggccgaatt
    20581 tgatgcgatc atccctttca atgatgattt gttccttttc aacgggggct gcacggtaga
    20641 tgcgaatggt tttatgacag ggggcgtatg ggggatcatg gatcggaaaa aacgccagct
    20701 ggtgctggat cctaactatg atttcattgg ggctatggat gtgcaaaacg gtgtgtatgc
    20761 agtgtataaa ggaggaaaat acctgtataa tggctttgtg tcaatgcccg ataaaaatag
    20821 taaaggaaaa tgggcattgg taaacggaaa agggatgcct tttacctctt atgcctttca
    20881 tgctataaat tctgtcaatt atgcaggctt cctggtgcag gatgcggcaa ctcgtttgtt
    20941 tggtatttat tcccggactg gtcaaaaaac aacggattgt atctacgaca ctatttacca
    21001 gtttcactgg aaggattcca ttgcagccgc ccgtaaaaac gggaaatggg gttttatccg
    21061 ctatgacgga cgggaaataa ttccgtttac ttacgagcag atagccgata cggattttga
    21121 taaaactttt atcatcgcca cccgtggcgg aaccacttat gtagtggaca aacagggaag
    21181 agaaatgctg gacccgcctg tgtccatcga tcagcagttc agtaatgcat tgcgtgcagc
    21241 caacaattca aaggaaagag gggcagcact gatcgcctat gatgatgcct taaaagcggc
    21301 cggttattcc gcagggcact gcagttacct ggtgggacaa aaaatgcagc aggtggtgga
    21361 catcgatttt catggtgtgt ttgaatattt catgaagcaa aaagtacctg ccaacggctt
    21421 tgtgggagcg ttgagtattc tcaaagctga acaccgttct gcgatcaaag ccattgctca
    21481 atatatcatt gatgaatatc ttgctcgaga aaaaggcctg gcgccccccg cctggcccgc
    21541 gggggtaccc aaagccggtt acggctgggg caagaccatg agcagcgatc gggtggtgcc
    21601 cgttactact gctccggcaa aacctgcaaa gacgaaaaag gaactggagg aagaattttt
    21661 aggaaagcgt tatttagtgg atgtcgccat cactgatggg ccgctttatg ggccaagaag
    21721 caaagccatg ctggcattcc ctaaaagagt ggatagtgaa aataatatcc tggtgtactt
    21781 ccagtacaac ctgccgggat ccaaccagaa ctgggctttc tatacggcag atgagttgcg
    21841 gcagttgcgt acgccaaaat gcaactatgt gccatgtagc tactgtggcg gcagcggggt
    21901 gcagaccagt aagtacaggc ataccaatga ttacagctac accttaggcc agaaacatat
    21961 ttatacgact accactacca gcgactgtaa caaatgctgc gggggtaaag tggtggtgaa
    22021 accctgcgta gaatgtgaat ggtagagaaa agtctgtatt ccttagtcgg gagtcttctt
    22081 ctgaagtaag ttataatttt aaaagatctt aatcatatca agacaatcta agatttttaa
    22141 tcactttctt ttttcttatt tgctatcact gcctttgtaa gcagccttgt cccaaaccgg
    22201 tccttaatat cgtcaaccgc tttaaagagt tgtaatttct ctatgttatt ttcaaataaa
    22261 ctcatttgca tggtaatggg tatcatatga ctaaagcgga cacccagtaa tcgcactggc
    22321 ctgcctttct gatatgattt ggtgaagagg tcttttactt ttgcaattaa catatcatcc
    22381 agtgcagtat agtcgactgt ttcttgtctt gatgtagttt caaaatctga gtatcttatt
    22441 ttaactgtta cacatccggt catcttttca tcttcccgca aagcataggc tgttttttct
    22501 gtaagccgta caagttcttt atgcagaaag tctaaatcgg tatagtctgt atcaaacgta
    22561 ttttcatggc tcatgctttt ttgctcccag tcggtagcaa tttctgtact gccccgccca
    22621 tgagctttat gccagagggc ttctccccat tttcccgcaa tccgttccat tatttccaca
    22681 ggtgttttgg caatatcttc tatggtgtaa agcccgaaac tcttcaggtg ttgttctgtt
    22741 tgtttgccaa caccgtttat tttttcgata cccaaaggcc acaaaaactc cagttccttt
    22801 ccatgaggaa tttgtaaaaa tccgttgggc ttggcttcat tggttgccat tttacttata
    22861 aacctcgcag atgaaagccc gcctgaaatt ggcagacctg tttctttggt gataagccgg
    22921 cgcagctcct gtgcaaaccg ggccgcacca aaatatttgt ccatgccggt gagatcaacg
    22981 taaaattcat caactcttgc tttttcaaac aagggttctt ttgctgcaat gatgtcagta
    23041 acccaacggg aatatttgct gtattgcccg ctgcttgcat gggttacgat agcatggggg
    23101 caaagctgca aggctttttt catgggcata gcagaatgaa tcccatattt tcgggcttcg
    23161 tagctgcagg tggaaacaac tcctctttca tagccgccaa tgagcactgg tttgcctttc
    23221 aatgagggat tgtttagtat ctctaccgat acgaaaaagg agtcaaggtc gaagtgggcg
    23281 atatgatgtt ggatgactga cactttcacc aaacgtaaag cttattgcaa atagatttcc
    23341 agtaaaccat caggtaagtt tacaactact tctttgttct tatgatttac tttccgcagt
    23401 gtttcttcat gcaagggtat caatacctcc ttgcctttaa tctccaagcg gcaaagcagc
    23461 tggtgaggct gttcgatcag ttctaatatt tcacctaatg attctttgtt attgataatg
    23521 gtgtagccaa gtagacttgc aggagctgat cttgcagcaa attttttaaa gtctggttca
    23581 ggtaaccaaa cctgcttctg agtaagcttt agtgcagatt ctcgcacatt aactccttcc
    23641 agcttaatat atatttcgtc ttccgacttg atttttgctg attctataaa ccaggggaga
    23701 aaagcgtttt tcttttcttc aatgaaaatt gcagccagcc ctttgagtga agttttttta
    23761 cccaactcat gtttcagcac caattcccct ttgagaccat gcacagcaac caattttcct
    23821 attttgaaat attccgtcat acagtacaaa gcaaaatata atcttctacc aaataaaaaa
    23881 atccttcccg gaatacggga aggattaaga actttcagga tgcggacgat ttatccttca
    23941 cttccgcctt caccttcagc ttttctctca ggtctgcgac ccggagcagc acgtctggct
    24001 cttttctggc ggtaagaatc ttcctggcgt cttttcacct gtgcttcatg ctcggcactc
    24061 cacttagtga atttttccat tgcggtagct tcatcaaaca aaccaagacg ctctaggtac
    24121 gcctttctcc aacagcccag at
//
LOCUS       DAORLS010000012        23916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1168_length_23916_cov_2.108503, whole genome shotgun sequence.
ACCESSION   DAORLS010000012 DAORLS010000000
VERSION     DAORLS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 23916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23916
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1168_length_23916_cov_2.108503"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK977_02070"
     CDS             complement(<1..297)
                     /locus_tag="PK977_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRF16918.1"
                     /translation="MLLIEFSSIEQYHAQLEEGSITCVQAVEHYLSRIADTRHLNAFV
                     HVYKEKALAKAKELDEYRKKGTPIGKLHGVVIGLKDVICYKDHPVTAASGILKDF"
     gene            complement(337..546)
                     /gene="tatA"
                     /locus_tag="PK977_02075"
     CDS             complement(337..546)
                     /gene="tatA"
                     /locus_tag="PK977_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRF16919.1"
                     /translation="MMQPVLLGVLGTNEIIIILIIVLLLFGGKKIPELMRGLGKGVRE
                     FNDAKSNVKKEIEESASDIKNSPNN"
     gene            complement(667..1032)
                     /gene="rplS"
                     /locus_tag="PK977_02080"
     CDS             complement(667..1032)
                     /gene="rplS"
                     /locus_tag="PK977_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRF16920.1"
                     /translation="MNAIAYIHEQLTGKKEFPAFKPGDNVTVNYKIIEGNKERIQSFK
                     GDVIKKQGNGATASFTVRKISDGVGVERVFPYYSPNIDSIVVNKTGHVRRAKLFYQRS
                     RSGKSARIREKRMVTESAE"
     gene            complement(1149..>1413)
                     /locus_tag="PK977_02085"
     CDS             complement(1149..>1413)
                     /locus_tag="PK977_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584642.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRF16921.1"
                     /translation="RREIMQQYLQRIKDLENLAMSYNGVEKAYAIQAGRELRVIVEAD
                     KVTDQDSDKLSFEIAQKIQTEMTFPGQIKVTVIREKRAVNVAR"
     assembly_gap    1414..1513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1514..2727)
                     /locus_tag="PK977_02090"
     CDS             complement(<1514..2727)
                     /locus_tag="PK977_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HRF16922.1"
                     /translation="MDPNILSVIIGVAALIAGLIVGKLIFAKNTRKKIEEAELQAQTI
                     LKEAELRAETIRKEKELEAKERFVQMKSAHDKEVFEKNKKINDTENRIRQKEQSINQK
                     EANLEKQVKENEAIKENLNRQIELVNIKRTELEKHQEEHIRRLEKIANLSADEAKTQL
                     VESLKHEAQTQALALQQEIIDEAKQKANKEARKIIIQTIQRTAAEQAIENSITVFNLE
                     SDEIKGQIIGREGRNIRAIEAATGVDLIVDDTPEAILLSSFDPLRREIARLSLQRLVA
                     DGRIHPARIEEIVEKTRKQIEEQVMEIGERTVIELGIHGLHKELVRMVGRMRFRSSYG
                     QNLLMHSRETANLCATMAAELGLNPKLAKRAGLLHDIGKVPDEESELSHALLGAKLAE
                     KYGENAAVVNAIA"
     assembly_gap    3021..3045
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3046..4122
                     /locus_tag="PK977_02095"
     CDS             <3046..4122
                     /locus_tag="PK977_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF16923.1"
                     /translation="SGNVKNLSYKADWVFLQYRSNGEWKTDSVQAKDGKYEFAGKIGE
                     PVQGRLRIKYGLGPDGKKVNMVNTRDMVSVFLQPGKIKITSIDSFSNVQVKGSPAHME
                     FSKLNALVKPYQEKMQPLLEKYSEYGKAKDTKNQSRVEAEIDAIDAEKNEKVYGEYVR
                     NNPNSPIALYALQQYAGWDINADKIEPLFNSLSIENKNYPSAADFKEKIDIAKKTGIG
                     RIAMDFTQNDTLGKPVSLSSFKGRYILVDFWASWCGPCRAENPNVVKVFHKYKDKNFH
                     IIGVSLDRPGQKERWMKAIHDDKLEWTQLSDLKFWDNEVAKQYGINAIPQNLLIDPEG
                     KIIAKNLKGEELEQKLGEALEGKK"
     assembly_gap    4153..4267
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4268..4448)
                     /locus_tag="PK977_02100"
     CDS             complement(<4268..4448)
                     /locus_tag="PK977_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRF16924.1"
                     /translation="MNELIAISALIGDRTYRIKINPADEEVVRKTLKVINEKILEFKT
                     HFAGKDMQDYIAMVLI"
     gene            4562..4816
                     /locus_tag="PK977_02105"
     CDS             4562..4816
                     /locus_tag="PK977_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16925.1"
                     /translation="MFVKVKSSVFIDALQLQLNAPKGECLRGVLKDDCGVVCRTLERE
                     IITDKEELTWEGLNDLPYGRYTLELSQGDDEMSLNLVKRV"
     gene            complement(4813..5103)
                     /locus_tag="PK977_02110"
     CDS             complement(4813..5103)
                     /locus_tag="PK977_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16926.1"
                     /translation="MNTAEQHLKRIQDKLQQLLKNYAAMQKENAKLKEELTDAQQKAS
                     AQQKVTDELKQQVSILKLSSGEMDEAEKKEFEKRINGYLKEIDRCIALLENN"
     gene            complement(5100..7526)
                     /gene="pheT"
                     /locus_tag="PK977_02115"
     CDS             complement(5100..7526)
                     /gene="pheT"
                     /locus_tag="PK977_02115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRF16927.1"
                     /translation="MTISYKWLSEYLPVTVDPERLSRILTSIGLEVESMRIYEEVKGG
                     LKGLVIGEVLSTEKHPNADKLTLTKVNIGSGEPLQIVCGAPNVAAGQKVVVAPVGATI
                     YPTAGDSLTMKVAKIRGVESHGMICAEDEIGLGTSHAGIMVLPEDARVGLPAADYFQP
                     FEDIVYEIGLTPNRMDAMSHWGVARDVCAYLSHHDKKDIKPKLPSSNNFKVDNTSFVI
                     EVKVENESACPRYSGVSIDKVTIKESPKWLQQKLKAIGQRPINNIVDITNFIQHETGQ
                     PLHAFDADAIGGRKVIVKFLPEASKFVTLDEKERKLSSEDLMICDEKEAMCIAGVFGG
                     LHSGVTEKTKNIFLESAWFNPVDIRKTSFRHGLRTDAASRFEKGTDISATVSVLKRAA
                     MLIKEVCGGEISSDIVDVYPSKKVKSEVAVKWHYIKKLSGKNYHPDAVKDILVSLGFE
                     IIKESVDELRVAVPYHKPDISLPADIVEEIVRIDGLDNIEIPEAITITPAVEENYSKE
                     IFREKIANYLVGLGFNEMMTNSITNAAYYAEEEQQNMVKMLNSLSAELNILRNSLFET
                     ALEVVGHNLNYKNNSLRLFEFGKAYSASGPGKYTENEQLCVVISGNKNDANWKQKAVN
                     SDFYFLKGIIESILKALGTEADTVETMQVDKLNNHIVFKRNGKVIAGAGEVKKAVLDK
                     FGIKQAVFFGGLNWTVLSEMAAGQTNAVKELPKYPSVERDLAMIVSKELQWESVEKTV
                     QKIKLNKLQEIRLFDIFESEKLGTGKKSIAVNFTFLDEEKTLTDKEIDGWMNKIMTTL
                     ENDLQAEIRK"
     gene            7867..8682
                     /locus_tag="PK977_02120"
     CDS             7867..8682
                     /locus_tag="PK977_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRF16928.1"
                     /translation="MANNLLKGKKGIIFGALDEKSIAWKTALRCYEEGAQIVLTNAPV
                     ALRMGEINKLAETCGNAPVIGADVTNMDDLKNLFEKSMEHFGGKIDFVLHSIGMSLNV
                     RKGKHYTEMNYEWNQKTLDISAMSLHRVLRTAWDMDALNEWGSVVALTYIAAQRVFPD
                     YNEMADAKALLESVTRSFGYHYGAKKKVRVNTISQSPTRTTAGSGVKGFDGFINYAEA
                     MSPLGNATAEDCANYCVTMFSDLTRYVTMQNLFHDGGFSFTGVTAGVIEKMDS"
     gene            complement(8769..8990)
                     /locus_tag="PK977_02125"
     CDS             complement(8769..8990)
                     /locus_tag="PK977_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRF16929.1"
                     /translation="MFWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVVENLQELE
                     DEGDVYETIEDIWPDYPTQDDFLFNEDEY"
     gene            9216..9881
                     /locus_tag="PK977_02130"
     CDS             9216..9881
                     /locus_tag="PK977_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF16930.1"
                     /translation="MITGKNIYKRFGTVEVLKGVDVNVSKGEVVSIVGPSGSGKSTLL
                     HILGTLDKADQGSVSMNNTNLSSLSGNKLADFRNKHIGFVFQFHHLLPEFTALENVCI
                     PGWLAGKKKKEVEERAKELLNLLGLSHRMQNKPGEMSGGEQQRVAVARALINKPDIVF
                     ADEPTGNLDSANARELHQLFFDLREKFSQTFLIVTHNEELARLSDRILYMKDGKIVEK
                     SVA"
     gene            complement(9893..10453)
                     /locus_tag="PK977_02135"
     CDS             complement(9893..10453)
                     /locus_tag="PK977_02135"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRF16931.1"
                     /translation="MALKIYTKTGDKGTTSLIGGTKVPKSHMRIEAYGTIDELNSYLG
                     LCFDLLTDEQSKKIIHEVQDRLFTIGSSLACDPIKEPKMRLPDLKEEDVQLLEKEIDR
                     IDSTIPPMQNFILPGGHTTVSHLHIARCICRRAERCCVRLELESLEVDEIILKYLNRL
                     SDYLFVLSRYIGHLLNVPEIPWKPRL"
     gene            complement(10522..11226)
                     /locus_tag="PK977_02140"
     CDS             complement(10522..11226)
                     /locus_tag="PK977_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF16932.1"
                     /translation="MNSIIHLENIRKSYFMGKHELEVLKGLSLEIFKNEYVALMGPSG
                     SGKSTLMNIIGCLDTPTSGTYILNNHDVSQMQDNALAEVRNKEIGFVFQQFNLLPRLT
                     AAENVALPLVYAGMSKKLRTEKAMHVLDMVNLTDRSHHRPNELSGGQCQRVAIARALV
                     NDPAIILADEPTGNLDTKTSYEIMEIFDQIFNNGNTIVIVTHEEDIANHARRIVRLRD
                     GVIESDRKNEKVIVHH"
     gene            complement(11297..>11495)
                     /gene="gatC"
                     /locus_tag="PK977_02145"
     CDS             complement(11297..>11495)
                     /gene="gatC"
                     /locus_tag="PK977_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRF16933.1"
                     /translation="QNMIVFVEKLNELDLDGVEPMLFMSDEVNVLREDEINGSVSREE
                     ALKNAPLHDKQFFKVPKVIKK"
     assembly_gap    11496..11544
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11737..12741
                     /gene="trpS"
                     /locus_tag="PK977_02150"
     CDS             11737..12741
                     /gene="trpS"
                     /locus_tag="PK977_02150"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRF16934.1"
                     /translation="MEKKKEIVMSGIRPTGFLHLGNYFGAVRNYVRMQEEFECYFMVA
                     DLHSLTTHPDTKELKTNVHRVLAENIACGLDPEKVALYCQSHVYETSELYLYLNMLAY
                     KGELEKTVTFKDKVRQHPDNVNAGLLTYPVLQTADIILHRAKYVPVGKDQEQHLEMAR
                     NFANRFNHRYGEVFPEPMAFNYGDELIKVPSLDGAGKMSKSINQYATLYLSDDDNLIR
                     KKVMKAKTDSGPTEPNSPKPDYIENIFLLMKLVSATDVVEKFESDYNNCTVRYGDLKK
                     QLAEDMVAFISPIRERAEHIRNDEKYLRDVMEKGAEKARTSAKATMELVRQAMGLNYF
                     "
     gene            12842..13480
                     /locus_tag="PK977_02155"
     CDS             12842..13480
                     /locus_tag="PK977_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRF16935.1"
                     /translation="MAKAKSKPKHIAVAGNIGAGKTTLTEMLSKHYKWIPNFEDVDHN
                     PYLMDFYEDMPRWSFNLQIYFLNSRLRQLVEIQKGTETVIQDRTIYEDANIFAPNLHE
                     MGLMSKRDFDNYFHFFQTLKTLVQPPDLLIYLNASVPTLVGQIQKRGREYEENIRLDY
                     LKRLNEYYNKWIADYKEGQLLVIDVDKNKFAEKDEDFGEIIRKVDAQLYGLF"
     gene            complement(13477..13995)
                     /locus_tag="PK977_02160"
     CDS             complement(13477..13995)
                     /locus_tag="PK977_02160"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HRF16936.1"
                     /translation="MILLASVPLQQFAQSRHEELLDWEANRRLTWADYKAKPDQNSDA
                     AASTTSILVISYNISSTGFTYKIECRFSKTRSWGLHKTDYILSHEQGHFDIAEIFARK
                     LHKKMSEYRFNKRTYQKDLDKIYEEVVKEKEKMQNDYDRETNHSINKEKQAEWLQNIR
                     QLLEEYKNYADY"
     gene            complement(14132..>14786)
                     /locus_tag="PK977_02165"
     CDS             complement(14132..>14786)
                     /locus_tag="PK977_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRF16937.1"
                     /translation="ASITENTRVSYPLHFISNAQEPSIGNLPKNIFFLTCDAFGVLPP
                     ISKLTPGQAMYQFISGYTAKVAGTEAGVTEPKPTFSACFGAPFLPLHPGKYAQMLGEK
                     MKKHNVNVWMVNTGWTGGPYGTGTRMKLKYTRAMITAALEGKLNNVSFDNDPVFGVAI
                     PTECPDVPSEVLHPRNTWKDKQAYDEKAKYLAGLFVNNFEKYASGVSADILAAAPRV"
     assembly_gap    14787..14990
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14991..15747)
                     /locus_tag="PK977_02170"
     CDS             complement(<14991..15747)
                     /locus_tag="PK977_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRF16938.1"
                     /translation="MSVPIMRFPVKNFIKLGLKNTESIHYQLSPEELVQDTLRIGEGV
                     LDENGALVIKTGEFTGRSPKDKFTVKDETTENSVHWNEFNIPIEPKYFDIVFKKITDY
                     IENLPELWVRDCFACADSRYRLNVRVVNEKPWNNLFAYNMFLRPSEEELENFEPAWHV
                     ISVPGLKLDPKECGTRQHNAAIVSFKHKMILIAGTGYTGETKKGIFTILNYILPHEKN
                     VLSMHCSANMGDDGDTAIFFGLSGTGKTTLSADP"
     gene            16033..>16900
                     /locus_tag="PK977_02175"
     CDS             16033..>16900
                     /locus_tag="PK977_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRF16939.1"
                     /translation="MKYLPLNPEIFVQNRQRFVSRMQKNSIAIFVSNDEVPSNGDAIY
                     SFKQNSDLYWLSGIEQEDSLVILFPDNPDPKYKEVLVLVRPNELKEKWDGKRLRVHEA
                     QAISGIKTIVWLDSLDGLLQPWIHLADNIYLDSNENDRKASLVRSRDYRFIDEMKSRY
                     PLHNYLRAAKLMKKLRGIKTALEVEVMQKAIDITENTFRRLLKFIKPGVWEYEIEAEI
                     MHSFLSQRATGQAYGSIIASGDRARTLHYVSNNAECKDGELILMDFGAEYGGYCADLT
                     RTVPVNGKFTRRQ"
     assembly_gap    16901..16912
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16913..17333
                     /locus_tag="PK977_02180"
     CDS             <16913..17333
                     /locus_tag="PK977_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRF16940.1"
                     /translation="TVYNTCLHLHDYAKSLLKPGISIIEYTDKVGEEATQQFLKIGLL
                     RKSDVKNEDPENRAYRKYLYHGISHHLGVDVHDLGTRTEPIKPGMVFTVEPGIYIEEE
                     QMGIRIENNLWITRNGNKDLMKNIPITVEEIETLMKK"
     assembly_gap    17575..17627
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17628..18197
                     /locus_tag="PK977_02185"
     CDS             <17628..18197
                     /locus_tag="PK977_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HRF16941.1"
                     /translation="VARLFKATSEMGKQLDSLSDVVSFGVAPGMIIYQFLRLSYAQDA
                     AGVEISILWLLPAFILPAAAAWRLARFNLDTSQSYTFKGMPVPAVGIFVASLPLIYWN
                     VNEIWVQQLLLNKWFHYGLVLVLSWLMVSTLPLMALKFKDYSVKNNLPKFLLAVIAIA
                     ALLLLKWMAIPAIIVAYVVLSLLFKNKAA"
     gene            18194..18439
                     /gene="purS"
                     /locus_tag="PK977_02190"
     CDS             18194..18439
                     /gene="purS"
                     /locus_tag="PK977_02190"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRF16942.1"
                     /translation="MTYTVQIKVMPLKELLDPQGKAVMGGLENLGLTGVEDVRVGKNI
                     TLQVNADSPEKARAIAEEASKKLLSNPVMEYFEVSVN"
     gene            18504..19169
                     /gene="rsmI"
                     /locus_tag="PK977_02195"
     CDS             18504..19169
                     /gene="rsmI"
                     /locus_tag="PK977_02195"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRF16943.1"
                     /translation="MLYLVPTPIGNLKDITLRALEVLKEVDLILAEDTRTTSHLLNHY
                     NITKPLSPYHQHNEHKVLQHLVNQLLEGKKMAVVTDAGTPGISDPAFLLVRECIKVGI
                     PVECLPGATAFVPALVNSGIPTNRFCFEGFLPLKKGRQTLLKQLTDEERTMIFYESPM
                     RLVKTLEEFITYFGEGRLCSVSRELTKLFEENKRGTLRDVVDYFSTKPVKGEIVIVVA
                     GKD"
     gene            complement(19166..19849)
                     /locus_tag="PK977_02200"
     CDS             complement(19166..19849)
                     /locus_tag="PK977_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRF16944.1"
                     /translation="MVKTKLIDGYPFITYAKQTFPTKDMMQRSKEFCQWMNQRRTVRE
                     FSSKPVPQKVIENIILTASTAPSGAHKQPWTFCVVSEPTLKKRIRQEAEKEELESYNG
                     RMPEEWLNDLRPLQTDWRKEFLETAPCLIIIFKRSYELEDEGHKHPNYYVTESCGIAC
                     GFLLAAIHHAGLAALTHTPSPMNFLSKILDRPENEKPFLLIPVGYPADECWVPDLQRK
                     PLEKIIRWY"
     gene            19891..>20135
                     /locus_tag="PK977_02205"
     CDS             19891..>20135
                     /locus_tag="PK977_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HRF16945.1"
                     /translation="MKKLIVLSVTLFLFVFSTSAQPDRWQQRVKYVMDIDMNVLTNQF
                     TGKQKLEYWNNSPDTLTKLFYHLYFNAFQPGSMMDTR"
     assembly_gap    20136..20171
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20172..21721
                     /locus_tag="PK977_02210"
     CDS             <20172..21721
                     /locus_tag="PK977_02210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF16946.1"
                     /translation="RVRDRIINLKPDEIGYQKIISLRMNGRPQLFKVLETILEVKLDK
                     PILPRSKVTLDMEFEAQVPLQVRRSGRDNPTSKVRYSMSQWYPKLCEYDYEGWHPTPY
                     VAREFYGVWGDYEVNITIDKKYILGGTGYLQNANQIGYGYENPGVKVNRPPGDKLTWK
                     FVAPNVHDFMWAADPEYKHVSRKIRDSLTIHVLYKNTNEPAKDWEDLLNMAERALPYI
                     EKTFGVYPYKQFSFIHGGDGGMEYPMATLCIGPGAALHEWLHSWYYGLLGTNEGLYPW
                     MDEGFTQYAEERVNAWMSNSSGFAQAGNYAAYYALVKSGKEEPMSTHADHYNTNFAYS
                     IASYSKGTVFIEQLGYITGAETRDRILLEYYKQWRFKHPNVNDFIRIAEKVSDMKLDW
                     YKEYWVNSTKTIDYSIDSLWEEGGKTKVRLNRIGQMPMPIDLQLTFKDGSKEIHYVPM
                     NLMYGEKPGEDSTITRKVYEPWRWTHSTYTIETNRKLADFSLVEIDPSLRMADVERRN
                     NRLELKW"
     gene            complement(21944..22591)
                     /locus_tag="PK977_02215"
     CDS             complement(21944..22591)
                     /locus_tag="PK977_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF16947.1"
                     /translation="MKEISVCIVDDNKELRNALEEIISMSEGYRCLGTIGTVEDAMNQ
                     LPILRPDVVLMDINLGTEESGIDIVRVLKPRIPDTNFMMCTVYEENEKIFEALSAGAS
                     GYILKKTDPGRMLEAIRELYEGGAPMSSQIARKVVAVFQQQQSATVEAESERLDMLSN
                     REKEILEFLSKGLMYKEIASQLFLSPETVRKHVYHIYEKLHVNNRVAAVNKYYGR"
     gene            complement(22649..23281)
                     /gene="rsmG"
                     /locus_tag="PK977_02220"
     CDS             complement(22649..23281)
                     /gene="rsmG"
                     /locus_tag="PK977_02220"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRF16948.1"
                     /translation="MDIILKYFSDFTPAQLGQFSKLEGLYKEWNEKINVISRKDIDSL
                     YEKHVLHSLSIAAAFDFADGSEIIDLGTGGGFPGIPLAIFFPEVKFHLVDSIAKKLKV
                     VEAVAGGIGLKNITTEHSRIENIKNRKFDFVVSRAVAPLKDLWKWSRPILKKDSTKMP
                     AGQKSGLICLKGGDLAAEIHDSGTKPRIMEINDIFPEEFFKEKYLLFVPV"
     gene            complement(23292..>23506)
                     /locus_tag="PK977_02225"
     CDS             complement(23292..>23506)
                     /locus_tag="PK977_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HRF16949.1"
                     /translation="AAAIIFFDWRYALAVFGIRLLLQAIIFYKSMKKMGEKDLWPYFI
                     FLDMWMFFYYLIFAPALWRKPAKGWS"
     assembly_gap    23507..23668
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23669..23899)
                     /locus_tag="PK977_02230"
     CDS             complement(<23669..23899)
                     /locus_tag="PK977_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HRF16950.1"
                     /translation="MLNKLIRFETFHTALQYLSYALAGIPYMGVGRNLSYRKSLFFKH
                     KGFSSINHIPSGDDDLFINKTATKSNTAVVIDP"
BASE COUNT         6545 a         4695 c         4941 g         6979 t
ORIGIN      
        1 aaaatctttt aatattccag acgcagcagt tacgggatga tccttatagc atatcacatc
       61 ttttaatcca ataactactc catgcagttt accaatagga gttccttttt ttcggtactc
      121 gtctaattcc ttagcctttg ctaaggcttt ctctttgtaa acatgtacga aggcattgag
      181 atgtcttgta tcggcgatgc ggctcagata atgctcaacc gcctgtacgc aggttatact
      241 tccttcttct agttgggcat ggtattgctc aatggaagag aactcaatca aaagcacttg
      301 cttttttggt gatcagatag aaatgaagaa taaaaatcag ttgttgggtg aattcttaat
      361 atcgctggca ctttcttcaa tctctttctt cacattgctt ttggcatcat tgaattcacg
      421 aacacccttt ccaagaccac gcatcagttc ggggattttc tttcctccaa acaataacaa
      481 gacaatgatg agaataataa tgatctcgtt tgttcccagt acacccagta atacaggttg
      541 catcataatc agataaattg agcggtaaag ataataagta cttaaaataa agccgcaaga
      601 acttgtagtt tcgaaaaaat aaagcccttc cggctggaag ggctttacaa ttaatttgaa
      661 agaaacttat tctgcagact cagttaccat gcgtttttca cgaatccttg cactcttacc
      721 agaacgactg cgctggtaaa acagtttggc acggcgaaca tgaccggttt tgttcacaac
      781 aattgaatct atattaggag aataataggg gaataccctt tctacgccta ctccgtcaga
      841 tattttacga acagtaaagc tggcagtagc gccattgcct tgtttcttga tcacatcgcc
      901 tttaaatgac tggatccttt ctttgttacc ttcaatgatt ttgtagttaa cagtcacatt
      961 atcaccgggt ttgaaggcag gaaactcttt ctttccggtc aattgttcgt gtatgtatgc
     1021 aatagcgttc ataatcctga tttttaggga gggcaaaggt aagcgaaaag ttgataagtt
     1081 ggttagtttt taagtttaaa agtttatcct ttttgctttt aaacctggtt acctgttata
     1141 cttaaaactt atctcgccac atttaccgcc cttttctccc tgattaccgt caccttaatc
     1201 tggccgggga aggtcatttc cgtctgaatt ttttgggcta tctcaaatga gagcttatct
     1261 gaatcctggt ctgtcacttt atcagcttct actatgaccc ttagttcacg gccagcctgg
     1321 atagcatagg ctttttccac tccattgtag ctcatagcca ggttttcaag gtctttgatg
     1381 cgctgcaggt actgctgcat gatctctctt ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnngctatgg cattcaccac cgcagcgttt tctccatatt tctcagccag
     1561 tttagctccc agtaaggcgt ggctcaattc actttcttca tctggtactt tgccaatatc
     1621 atgcagcaga ccggctcttt tagccagttt tgggttcagt cccagttcgg cagccattgt
     1681 agcacaaaga ttggctgttt cccgactgtg catcagcagg ttttggccat aagaggagcg
     1741 gaaccgcatc cttcccacca ttcttaccaa ttctttatgg aggccatgaa tgcctagttc
     1801 aatgacagtt ctttcgccga tctccattac ctgctcttca atttgcttac gggtcttttc
     1861 aacaatttct tcgatacggg cagggtggat acgaccatcg gccaccagtc tctgcaaact
     1921 caggcgggca atttcccggc gcagcgggtc aaagcttgat aaaagaatag cttcaggagt
     1981 atcatccaca atcaggtcaa caccggtggc tgcttcaata gcccggatat tccggccttc
     2041 acgaccaatg atctggcctt tgatctcatc actttccagg ttaaaaacag taatggagtt
     2101 ttcaattgcc tgttcagctg ctgtgcgttg tatagtctga ataatgatct ttcttgcttc
     2161 tttattcgcc ttttgcttag cctcatcgat gatttcctgc tgcaaggcaa gggcctgcgt
     2221 ttgggcttca tgcttcaggc tttctaccag ttgtgttttt gcttcatcag cagaaagatt
     2281 agcgattttt tccagcctgc gaatatgttc ttcctggtgt ttttccagtt cggtgcgttt
     2341 gatatttacc agctctattt gccggttcag attttcctta atagcctcat tttcctttac
     2401 ctgcttttca agattagcct ctttttgatt aatggattgc tctttctggc ggatacggtt
     2461 ttctgtatca ttaatctttt tgttcttttc aaacacctct ttgtcatggg cagacttcat
     2521 ctgcacaaaa cgctcctttg cctccagttc cttttctttt ctaatggttt cagctcttaa
     2581 ttcggcttct ttcaggattg tctgggcttg taattcagcc tcttctattt tttttcttgt
     2641 attcttggca aaaatgagct tacccactat gaggcccgca ataagggcag ccacgccaat
     2701 gatgacggaa agtatattcg gatccatgaa tctttttgtt taagtgtcgc acaatgctga
     2761 ctgttaataa taaattcata ctcttgtcag tatgtaatta gcgaagatac aaaacagggg
     2821 cttatgttag aaattgtact actattatgt gccgttcaaa taaaaatggg taaaactgag
     2881 acatatgccg cctctgagca gcgctttttt gtaatcttgc aacactaaaa ccgatttttt
     2941 acatgaaaaa agccgttcta gccctggtta tgctgccttt agctgttatt tcgcaggata
     3001 agcaaaagtc tttcaaaatc nnnnnnnnnn nnnnnnnnnn nnnnnagtgg caatgttaaa
     3061 aacctgtctt ataaagcaga ctgggttttc cttcaataca ggagcaacgg agagtggaag
     3121 actgatagtg tgcaggcaaa agacggaaaa tatgagtttg cagggaagat aggagagcct
     3181 gtacaaggaa ggcttcggat taagtatggc ctgggccccg atggtaaaaa ggtgaatatg
     3241 gtgaatacaa gggatatggt gtctgttttt ttacaaccgg ggaaaataaa gattacctca
     3301 atcgattctt tcagcaatgt gcaggtgaaa ggttctccgg cacatatgga attttccaaa
     3361 ctgaatgccc tggtgaagcc ttaccaggaa aaaatgcaac cattactgga gaaatatagc
     3421 gaatatggaa aagcaaaaga cacgaagaat caatccagag tggaagctga aattgatgca
     3481 attgatgcag aaaaaaatga gaaagtatat ggtgaatatg taaggaataa tcccaactcc
     3541 ccgattgcct tatatgcact gcagcaatat gctggctggg atataaatgc ggacaagata
     3601 gagccgctct ttaattcact ttccattgaa aataaaaact atccttctgc ggcggacttt
     3661 aaagaaaaga ttgacatagc aaagaagact ggtattggca ggatagctat ggattttacc
     3721 caaaatgata cattgggcaa gccggtttct ctttcctcct ttaaaggtcg ttatattctg
     3781 gttgatttct gggcaagctg gtgtggtccc tgccgtgctg aaaatcccaa tgtggtgaaa
     3841 gtgtttcata agtataaaga caagaatttt catattatcg gcgtttcact ggaccggccc
     3901 gggcaaaagg aaagatggat gaaggctatc catgatgata agttagagtg gacccaactc
     3961 agtgatctga aattctggga taatgaggtg gccaagcaat atggtatcaa tgctattccc
     4021 caaaacctgt tgatagatcc ggaaggtaaa ataattgcta aaaaccttaa aggggaggaa
     4081 ctggaacaga aactaggcga agcactagaa ggaaaaaaat aatcataaaa gtaaaaccgg
     4141 ctgttgccgg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnaga tcagtaccat ggcaatataa tcctgcatat cttttcctgc aaaatgcgtt
     4321 ttgaactcaa gtatcttctc gttaatgact tttaaggtct ttctgactac ttcttcatca
     4381 gccgggttga tcttaatccg gtacgtacgg tcgccaataa gagcagaaat tgcaattaat
     4441 tcattcataa tgctggtatt ttcaaaatta gttctctata ttcggcatcc ctttcaaacc
     4501 catctatact ccctgaaaac aaggttaagg acacccaacg gaaacggttt aatcgtttac
     4561 gatgtttgtt aaagttaaat catctgtttt tattgatgca ttgcagttgc aacttaatgc
     4621 accaaaagga gaatgcctgc gtggcgtttt aaaagatgat tgtggtgtag tctgtagaac
     4681 ccttgaaaga gaaatcatta ctgataaaga agaattaaca tgggaaggct taaatgatct
     4741 gccttacggc cgttatacgc tggagctttc tcaaggcgat gatgagatga gcttaaatct
     4801 cgttaagagg gtttaattat tttccagcaa ggcaatacag cgatcaattt ctttcaggta
     4861 gccatttatt cgcttttcaa actctttctt ttctgcctca tccatttcgc cactgctgag
     4921 tttaagtatg ctgacctgtt gttttagttc atccgttact ttttgttgag cagaagcttt
     4981 ttgttgtgca tctgtcaatt cttctttgag ctttgcattc tctttttgca tagcagcata
     5041 attcttcagc aactgctgaa gtttgtcctg tatgcgtttg agatgttgct cggcagtatt
     5101 cattttctta tttcggcctg caggtcattt tcaagagtgg tcataatctt gttcatccag
     5161 ccatcaattt ccttgtctgt gagggttttt tcctcatcta aaaaagtgaa attgacagca
     5221 atcgatttct ttccggttcc taatttttca ctttcgaata tatcaaacag tcttatctct
     5281 tgtagtttat tcagcttaat cttttgcact gttttttcta cactttccca ctgcagttcc
     5341 ttggatacga tcattgccag gtctctttca acagaagggt attttggaag ttcttttacg
     5401 gcatttgttt gtcctgcggc catttctgac agcactgtcc agttcaatcc cccaaaaaat
     5461 acagcttgtt ttatgccgaa tttatctaag actgcctttt ttacttctcc ggctccggca
     5521 atcactttgc catttctttt aaaaactatg tgattattaa gtttgtcaac ctgcatggtt
     5581 tcaacagtat cagcctctgt gcctaaggct ttcaagatgg actcaataat acccttcaag
     5641 aaataaaaat ctgaattaac agctttttgt ttccaattgg catcattctt atttccggaa
     5701 attactacac atagttgttc gttttcagtg tacttgcctg ggccggaagc actgtaggct
     5761 tttccaaatt caaacaggcg caaagagtta ttcttataat taagattgtg gcccaccact
     5821 tccagcgccg tttcaaagag tgaattccgc agtatgttta attcagcact caggctgttc
     5881 agcatcttta ccatgttttg ctgttcctct tcagcatagt aagccgcatt ggtaatagag
     5941 ttggtcatca tttcgttgaa tcccaggcca acgaggtaat tggcaatttt ttccctgaaa
     6001 atttccttgg aatagttttc ttccacagcc ggggtaatgg taatggcttc ggggatttcg
     6061 atattatcaa ggccgtcaat tcgaacgatt tcttctacaa tgtcagccgg aagacttata
     6121 tccggcttgt ggtaaggtac agctacccgc agttcatcca cactttcttt gatgatctca
     6181 aatccaaggc tgactaaaat atctttaact gcatcagggt gatagttctt gccgctcagt
     6241 tttttgatgt agtgccattt tacagccacc tcggacttta ctttttttga cgggtagaca
     6301 tctacaatgt cagaagaaat ttcgccgccg catacttctt tgatcagcat tgccgctctt
     6361 ttcagcacag atacggtagc ggaaatatct gttcctttct caaaacggct ggcagcatct
     6421 gtacgcaatc catgccggaa agaggttttc ctgatgtcca cggggttgaa ccaggcactt
     6481 tccaggaaga tgttctttgt tttttctgta acgccactat gcaatccgcc aaatacccct
     6541 gcaatacaca tggcttcttt ttcatcacag atcatcaggt cttcactgct cagttttctt
     6601 tccttctcat cgagagtaac gaatttgctt gcttccggaa gaaattttac aatcactttt
     6661 ctgccgccaa tggcatctgc atcaaaagca tggagtggct gccctgtttc atgctgtatg
     6721 aagttggtaa tgtctacaat attgttgata ggtcgctgac caatggcttt cagcttctgc
     6781 tgcagccatt ttggcgactc tttaatggtt actttatcaa tactgacacc ggaatacctg
     6841 ggacaggctg actcattctc aaccttcacc tcaatgacga aggaggtatt atctacttta
     6901 aaattattgc tgctaggaag ttttggtttt atatcctttt tgtcatggtg tgacagataa
     6961 gcacacacat ctcttgccac tccccaatgg ctcatggcat ccatccggtt gggggtaaga
     7021 ccgatttcat agactatatc ttcgaaaggt tgaaaataat cggcagcagg aaggccaacc
     7081 ctggcatctt ccggcaacac cataatgccc gcatgagatg tacctaagcc aatttcatct
     7141 tccgcacaga tcataccatg gctttccacg cccctgatct tcgccacttt cattgtgagg
     7201 gaatcaccag cagtgggata tatagttgct ccaaccggtg ctaccactac tttctgccct
     7261 gctgctacat tgggtgctcc gcaaacgatt tgtaagggtt cacctgaacc tatattgact
     7321 ttggttagtg ttagtttatc ggcattaggg tgtttttcag tactcagtac ttcaccaatc
     7381 actaatcctt tcaatcctcc ttttacttct tcgtagatac gcatactttc cacttctaag
     7441 ccaatggaag taaggatgcg gctcagtctt tcggggtcaa cggttacggg taaatattca
     7501 ctcagccatt tataagagat cgtcatatcc ggtagcttct tttattgctg cgaagataaa
     7561 ggaaaggggg gaagtgcttt ttggaccgta tcccgtttat ttgtacttca aattttaata
     7621 ttgaaaataa agggatttgt tatactcgga ataattttgt tttcattact tcccgtttac
     7681 atggtgtaca aatacctgca acgggtcatt agacctagag aatctttaca acgaattttc
     7741 ctctacctgc ttgttgtatt tattctcatt ttcggctata cctttcttct ggtttttgtg
     7801 atcaggctta tgtttcccgg agcttgagaa gaagttccta tttttaccac tacaaaatca
     7861 actgatatgg caaataactt actgaaaggg aagaaaggga tcatcttcgg agccttagat
     7921 gaaaaatcca ttgcgtggaa aacggcttta cgctgctatg aagagggtgc gcaaattgta
     7981 ttgaccaatg cgcctgtagc acttagaatg ggagagataa ataagctggc tgagacctgt
     8041 ggtaatgcac ccgtaatcgg tgctgatgtt accaatatgg acgacctgaa gaacctgttt
     8101 gaaaagtcaa tggagcactt tggtggcaaa attgattttg ttttacactc catcggtatg
     8161 agcctgaatg tgcgcaaggg aaagcattat acagaaatga attacgagtg gaaccagaaa
     8221 acactggata tatcggctat gagccttcac cgggtgctgc gtactgcctg ggatatggat
     8281 gccttgaacg agtggggaag tgtagtagca cttacctata tagcagcgca gagagtattt
     8341 cccgattaca acgaaatggc cgatgccaaa gcgttgctgg aaagtgtaac gagaagtttt
     8401 ggctaccatt atggtgcaaa gaagaaagtc agggtgaaca ctatctccca gtcgcctacc
     8461 cgaaccacag ccggcagcgg cgtaaaaggg tttgacgggt ttattaatta tgcggaggca
     8521 atgagtcctt tgggcaatgc cactgcggaa gattgtgcta attattgtgt taccatgttt
     8581 agcgacctca ccaggtatgt gaccatgcag aacctcttcc atgatggtgg tttcagcttt
     8641 acaggtgtta ctgccggtgt aattgaaaaa atggatagct gataatagga atcaggacct
     8701 aattcaaaaa gccgctaatt caagcggctt ttttcataca aatcattttt tatcggtatt
     8761 atcaattgtc agtattcatc ctcattgaag agaaaatcat cttgggtagg atagtccggc
     8821 caaatgtctt cgatggtttc atatacatca ccctcatctt ccagttcctg caggttttct
     8881 accacttcta taggggcgcc gctacgaata gcataatcga tcagctcatc tttggtggct
     8941 ggccaggggg catcttcgag gtacgaggct aattctaaag tccaaaacat cttctcaggg
     9001 ttttaaatgg cgaataaggt tttcgttacc aatatcgagg ggtactaccc ccttaatttt
     9061 ccgcaaaagt agttgtatta acgaattttc caaatcctta gttgtacacg gttttttgaa
     9121 ggatttctta aaaaaaaact ccgacaaata aatacagttt ttgccgctgc tttgttttct
     9181 ggatatttgg ataaattaat tgttacagaa gctgcatgat aacaggaaaa aatatataca
     9241 aacggttcgg aacggtggaa gttttgaagg gagttgacgt aaatgtgagc aaaggggaag
     9301 ttgtttccat tgttggccct tccggatcgg gaaaaagcac tttgttgcat attctcggaa
     9361 cattagataa ggcggaccag ggatctgtca gtatgaataa tacaaatctt tcatccctgt
     9421 ctggcaataa actggctgat ttccggaaca aacacatcgg atttgttttt cagtttcatc
     9481 atttattacc cgaatttacc gcattggaaa atgtttgtat ccccggctgg ctggccggca
     9541 aaaagaagaa agaagtagaa gaaagggcaa aggaactgct caacttgctg gggctttcgc
     9601 atcgtatgca aaataagccc ggggaaatga gtggtggtga gcaacagcgg gtggccgtcg
     9661 caagagcatt gataaataag ccggatatcg tttttgccga tgagcctacg ggcaatcttg
     9721 attcggccaa tgcacgggaa ctacaccagt tgttttttga tctcagggaa aaattcagcc
     9781 aaacatttct gatcgttact cataatgaag aactggcccg gttaagcgac cggattttgt
     9841 atatgaaaga cggtaagatt gtagagaagt ctgtagccta aagctagatt tgttacaacc
     9901 gtggtttcca gggtatctca ggtacattca gcaaatggcc tatgtagcga cttagcacaa
     9961 acaagtaatc actaagcctg ttcaggtatt ttaaaatgat ttcatccact tcgaggcttt
    10021 ccagttctaa tcttacacaa catctttctg ccctgcggca aatacaacgg gcaatatgca
    10081 gatgagaaac ggttgtgtgg cctcccggca gaatgaaatt ttgcatggga gggattgtac
    10141 tatctatcct gtcaatttct ttttcgagca attgcacatc ctcttctttc agatccggta
    10201 atctcatttt tggctcttta ataggatcac aggctagcga tgaaccgata gtaaacaatc
    10261 tgtcctgcac ttcatgaatg atctttttgc tttgttcatc agtcaacaaa tcgaaacaca
    10321 aacccaggta agaatttagt tcatcaatag ttccataggc ttcgatacgc atatgtgatt
    10381 tgggtacctt ggtgccccca atcaatgaag tagtgccttt atcgcctgtt tttgtataaa
    10441 tttttaatgc catgtcaagt tcaaagatgc ctgaagtaac aaaaatttcc ggtcagggtt
    10501 cagaaaaggg gaaacaattt cttaatgatg cacaattact ttttcatttt tcctgtcact
    10561 ctcaatcacc ccgtctctca gccttacaat cctgcgggca tgattggcaa tatcttcttc
    10621 gtgggtaaca attactatcg tatttccatt attgaaaatc tggtcaaata tttccatgat
    10681 ctcataggaa gttttggtat ccaggttacc tgtcggctca tctgccagga taatggcagg
    10741 atcattcacc aatgccctgg caatagccac ccgctggcat tgtccgcctg ataattcgtt
    10801 gggacgatgg tggctgcggt cggtcaggtt taccatatcc agtacatgca ttgccttctc
    10861 tgttcttagc ttcttgctca tgccggcata taccagtggc agggctacat tttcggcggc
    10921 ggtcagccgg ggtaaaagat tgaactgctg aaatacaaac ccgatttctt tattgcgtac
    10981 ttctgccaat gcattatcct gcatctggct aacatcatga ttgttcaaaa tgtatgtccc
    11041 tgatgtcggt gtatcaaggc agccgataat gttcatcagg gtgctcttgc cagagccgga
    11101 gggccccatc agggctacat attcgttttt gaaaatttca agggatagcc cttttaatac
    11161 ttccagttca tgtttgccca tgaagtagct cttgcggata ttttcgagat ggatgatact
    11221 gttcatgaag ttaaatgtag gaaaccagag gtcagatgtc gtgataaatg gaagtatcgg
    11281 acgacatacc tcagcctcac ttttttatca ctttaggcac tttaaaaaat tgtttgtcat
    11341 gcaaaggagc atttttcaaa gcttcttccc ggctaacgga tccgtttatt tcatcttctc
    11401 ttaaaacgtt cacttcatcg ctcatgaaaa gcattggctc tacgccatcg agatctaatt
    11461 cattcagctt ctcaacaaag acgatcatgt tttgcnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnactgca aacgggaaag gtgggctagt ttttcgacca
    11581 atgcatcggt tacttccata ggacaaagat agtcaggagt cgggagttag gagtcaaatt
    11641 gaagcgtagg gaaaaatgga aacagcaaaa tgatcgggat tttcctgtcg gctgctgctt
    11701 aatgactcct tactatttca ttatcttcgc cgcctcatgg aaaagaagaa ggaaatagta
    11761 atgagcggca tccggcctac cgggtttttg cacctgggaa actactttgg tgccgtaaga
    11821 aattatgtgc ggatgcagga agaatttgaa tgctatttca tggtggccga tctgcattcg
    11881 ctgaccaccc acccggatac caaagagcta aaaaccaatg tacacagggt gctggctgaa
    11941 aatattgcct gtggcctcga tccggaaaaa gtagccctgt attgccaaag tcatgtatat
    12001 gagacttcgg agctatacct ctacttaaat atgctggctt ataaaggaga gttagaaaaa
    12061 acagtcactt ttaaagacaa agtgagacag catcctgata atgtgaatgc gggactactg
    12121 acttaccccg tcctgcaaac tgctgacatt attttgcaca gggccaaata tgtgccagtg
    12181 ggtaaggacc aggagcagca cctggaaatg gctagaaatt ttgcaaaccg gtttaaccat
    12241 cgttatggcg aggtttttcc ggaaccgatg gcttttaatt atggcgatga gctgataaaa
    12301 gtgcctagcc ttgacggagc cggtaaaatg agcaaaagca taaaccagta tgcaacgtta
    12361 tacttgtccg acgatgataa cctgattcgt aaaaaggtta tgaaagctaa aactgacagc
    12421 gggccaaccg aacccaattc tccgaaaccg gattatatcg agaatatctt tttgttgatg
    12481 aaactggtaa gtgcgacaga tgtggtggaa aaatttgaat cagattataa taattgtacc
    12541 gtccggtacg gggatttgaa aaagcaactg gccgaagata tggttgcctt tatttcaccc
    12601 atcagggaac gagcagagca tattcgtaat gatgaaaaat acctgagaga tgtgatggaa
    12661 aagggtgcag aaaaggccag gacaagtgct aaagctacca tggaattggt acgacaggcc
    12721 atggggttga attattttta aaaaatcaga ataacaaatc tcaaattcca aaaaagggtt
    12781 tactttgaac cttccgcgga gtcggaactt gaaatttgtt tcttttggaa tctatccagt
    12841 tatggcaaaa gcaaaatcta aaccaaaaca tatagcagta gccggaaaca ttggtgccgg
    12901 taagaccacc ttgacagaaa tgctgagcaa gcattataag tggataccca actttgagga
    12961 tgtagaccat aatccttatc tcatggattt ttatgaggat atgccgaggt ggagttttaa
    13021 cctgcagatt tactttttga acagccgtct tcgccagctt gtagaaatac agaagggaac
    13081 tgaaacggtt atccaggaca gaacaatata tgaggatgcc aacatctttg cccccaacct
    13141 gcatgaaatg gggctgatga gcaaaaggga ttttgataac tacttccatt ttttccagac
    13201 tttaaaaaca ctggtgcagc ccccagacct cctgatctat cttaatgctt ctgtacctac
    13261 actggtaggc cagatacaaa aaaggggaag agaatatgaa gagaatatcc ggctggatta
    13321 cctgaaacgc ctcaatgaat attataataa gtggattgcc gattacaaag aaggacagtt
    13381 gctggtaatt gatgtggaca agaacaagtt tgctgagaaa gatgaagact ttggagagat
    13441 catccgtaaa gtagacgcac aactgtacgg cctgttttag tagtctgcat aatttttata
    13501 ttcttcgagc agctgccgga tattttgcag ccattctgcc tgtttttctt tgttgatgct
    13561 gtgattagtt tctctgtcgt aatcgttttg cattttttct ttctctttta cgacttcttc
    13621 gtaaatttta tccaggtctt tttgataagt tcttttattg aacctgtatt cactcatttt
    13681 tttgtgcagt ttccgggcaa atatttctgc aatatcaaaa tggccctgct catggctcag
    13741 aatataatct gttttgtgaa gtccccacga acgggttttg gaaaacctgc actctatttt
    13801 gtacgtaaat ccggttgatg aaatattata agaaattacc agtatggagg tagtagaagc
    13861 tgcggcatca gaattttgat ccggcttcgc tttataatca gcccaggtga gccttctgtt
    13921 tgcctcccaa tcaagcagct cttcatgccg ggattgagca aattgttgca gaggaactga
    13981 tgctaataat atcaatatga gactgtactg ggatttcatc aattataatc aacgtaaagt
    14041 cggaataatt gttttgccgg ctgcttaatg cggcgttaaa aataaaaccc ctgcagttgc
    14101 aggggtttag caagcaatcg tccggaaaaa attatactct tggtgctgca gctaatatat
    14161 cagctgacac acctgatgcg tatttttcaa aattatttac aaataggcca gcaaggtatt
    14221 ttgccttttc atcgtacgcc tgtttgtcct tccaggtgtt ccggggatgc agcacttcag
    14281 atggtacatc agggcattca gtggggatag caacaccaaa aacgggatca ttgtcaaaag
    14341 aaacattatt aagttttcct tccagtgctg cagtaatcat cgcccgggta tatttcaatt
    14401 tcatacgggt acctgttcca taagggccgc ctgtccaacc ggtattcacc atccatacat
    14461 taacattatg ttttttcatc ttctcaccca gcatttgtgc atatttaccg ggatgtagtg
    14521 gcaggaacgg agcaccaaaa caagcactga aagtaggttt tggttctgtc acaccggctt
    14581 ctgtgccagc tactttggcc gtatagccgg aaatgaactg gtacatagcc tgtcccgggg
    14641 ttaacttaga aataggaggc aagacaccaa atgcatcgca ggtcaggaaa aatatattct
    14701 taggcaaatt gccaatggaa ggttcctgcg cattacttat aaaatgcaac ggataagaca
    14761 ccctggtgtt ttctgttatc gaagcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taggatcggc
    15001 gcttaatgtt gttttaccag ttccgctgag accaaagaag atagccgtgt caccatcgtc
    15061 gcccatgttg gcactgcaat gcatactcag cacatttttt tcatgaggta aaatgtaatt
    15121 gagaatggtg aaaatacctt ttttggtctc gccggtatat cctgtgccgg ctatgaggat
    15181 cattttatgt ttaaatgaaa caatagctgc attgtgttgc cgggtaccgc attcttttgg
    15241 atctaatttc agaccgggaa cactaattac atgccatgct ggctcaaaat tctccagttc
    15301 ttcttcggaa gggcgaagaa acatattgta tgcaaagaga ttgttccatg gcttttcgtt
    15361 tactaccctg acattgagcc tgtaacggga atcggcacag gcaaaacagt cacgaaccca
    15421 caactctggc aggttttcaa tgtaatcagt tattttttta aagacgatat caaaatattt
    15481 aggctcaatc gggatattaa actcattcca gtgtaccgag ttttcagttg tttcgtcttt
    15541 cacagtgaat ttatcttttg gggagcgacc ggtaaattca cctgtcttaa tgacaagggc
    15601 gccattttca tccaatactc cctcgccgat acgaagggtg tcttgaacca attcttccgg
    15661 tgataattga taatgaatgg attcagtgtt tttcagtccc agttttataa agttcttcac
    15721 agggaatcgc atgataggaa cagacatagg tgcaaatcgt tttgttgaag gcacaaaggt
    15781 aaggggcaaa acaatttaaa ggcaaacaag tgttagtgtc acacattgaa aaaactttta
    15841 aatatttctc cgacaattaa tatttgttca ctatctcgtt tgtaggcttg attttattga
    15901 aaaggggtga attgccatta gattccatcc attttagtca ctcgcaacag gatttgtgat
    15961 gaatgtcagt tgccggaagt atttgctctt tatctttaga cctatcaata ataaccatta
    16021 caacagctaa tcatgaaata tctgccacta aaccccgaaa tatttgtaca gaaccgtcag
    16081 cggtttgtaa gccggatgca aaagaattcc atagccatat ttgtaagtaa tgatgaagtg
    16141 ccaagtaacg gcgatgctat ttattccttt aaacaaaaca gcgacctcta ctggcttagc
    16201 ggtattgagc aggaagatag tttggtgata ttattccctg ataatcctga tcctaaatac
    16261 aaggaagtgc tggtattggt acgccccaac gaattgaaag aaaaatggga tggcaaaaga
    16321 ctaagagtgc atgaggcgca ggctatcagc ggtataaaaa ccatagtatg gctcgatagc
    16381 ctggacggtt tgctgcaacc ctggattcat ctcgcagaca atatttacct cgatagtaat
    16441 gagaatgacc ggaaagcaag cctggtccgt tcaagggatt accgctttat tgatgaaatg
    16501 aaaagccgat acccattgca taattattta cgggctgcaa aactgatgaa aaaactccgt
    16561 ggtattaaaa ccgcattgga agtagaggtg atgcagaagg caattgatat cacagaaaat
    16621 acatttcgca ggctgctaaa atttatcaaa ccaggagtgt gggagtatga aattgaagct
    16681 gaaatcatgc attccttcct gagccagcgg gcaaccggac aggcttatgg cagcatcatt
    16741 gccagtggtg acagggccag aacattgcat tacgtttcaa acaacgccga gtgtaaagac
    16801 ggagaactta tcttaatgga ttttggagct gagtatggtg gttattgtgc tgaccttacc
    16861 agaactgttc cggtgaacgg aaagtttacc cgcaggcaaa nnnnnnnnnn nnaacggttt
    16921 ataatacttg tctgcatttg catgattatg caaaaagcct gctgaagcca ggcatcagca
    16981 ttatagaata caccgataaa gtgggcgaag aagcaacaca gcaattttta aaaattggtt
    17041 tgctaagaaa atctgatgta aagaatgaag acccggaaaa cagggcatac cgcaaatatt
    17101 tgtatcatgg tatctcacat catttgggtg tggatgtgca tgacctgggt accagaactg
    17161 aacctataaa gcccggaatg gtatttacag tggaacctgg tatttacatt gaagaagagc
    17221 aaatgggcat ccgcattgag aataatctct ggataaccag aaacggaaat aaagacctga
    17281 tgaaaaatat acctattacg gtggaagaga tagaaacgtt gatgaagaag taataagttc
    17341 actgcaatgg ttcatagttc atggtttttc ctattattgc aggggatcgt ggcacatcgc
    17401 cagaaacttt gaaccatgaa caaaaagata tgaaacaagt cccgaatatt tttacccttc
    17461 tgaaccttat atttggttgc ctcgccattg tggcaacact ccaaaacggt atcataatca
    17521 atgaaacgtc tgaaggggtg caattgatcg acattactga aaaaatatgg ctggnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta gcccgtcttt
    17641 ttaaggcaac atcagaaatg ggaaagcaac ttgattcgtt gtccgatgtg gtgagttttg
    17701 gtgttgctcc tggtatgatc atttatcagt tccttcgcct cagttatgca caggatgctg
    17761 cgggggtaga aatatccata ctctggttgc tgcccgcttt tatccttcct gctgcggctg
    17821 catggcgttt agcaaggttt aatcttgaca ctagccaatc atatacattt aaagggatgc
    17881 ctgttccggc ggtgggcatt tttgtcgctt cactaccgct tatatactgg aatgtgaatg
    17941 agatctgggt tcagcaactc ctgcttaata agtggtttca ttacgggttg gtactggtgc
    18001 tttcctggct tatggtttcc actttaccat tgatggcatt aaaatttaaa gattacagtg
    18061 taaaaaataa tttgccaaaa tttttgctgg ctgtcattgc tatagcagcg ttgttacttc
    18121 tgaaatggat ggccattccg gcaataatag tggcttatgt tgtcctatct ttgcttttca
    18181 aaaataaagc agcatgacct atacagtaca gataaaagta atgccactga aagaattgct
    18241 ggatccacag ggtaaagccg ttatgggtgg tttggagaac ctgggattaa caggagtgga
    18301 agatgtaagg gttggtaaaa acatcacttt acaggtaaat gccgattctc ccgaaaaagc
    18361 cagggctatt gctgaggaag caagtaaaaa attactgagc aacccggtga tggagtattt
    18421 cgaagtctct gttaattagt tgactggttg ataggttgac aggagttata cttatcaacc
    18481 taggaacaaa tcaactttca actatgcttt atttagttcc cacaccaata ggtaatttaa
    18541 aagatattac ccttcgggca cttgaagtgc tgaaggaagt tgatctgatt ttggcagagg
    18601 atacccgaac tacatctcat ttgttgaatc attacaatat tactaaacca ctttcgccct
    18661 atcatcagca caatgaacac aaagtgctgc agcatttggt caaccagtta ctggaaggaa
    18721 aaaaaatggc tgtggttacg gatgcaggca cacccggaat ctctgacccg gcatttttgt
    18781 tggtgagaga gtgtattaag gttggaatcc ccgttgagtg tttacctggc gctactgctt
    18841 ttgtacctgc cctggtgaac agcggcatac ccacaaaccg gttttgtttt gaagggtttt
    18901 taccgctcaa aaagggaaga cagactttgt tgaagcaact tactgatgaa gaaaggacca
    18961 tgatttttta cgaatcgccg atgcggttag tgaaaacgct ggaagaattc atcacttatt
    19021 ttggtgaagg aaggctatgc agtgttagcc gggaattaac gaagcttttc gaggaaaata
    19081 agcgaggcac acttcgggat gtggttgatt acttcagcac aaagcctgta aaaggagaaa
    19141 ttgtaattgt ggttgcaggc aaagattaat accagcggat aattttttct aacggctttc
    19201 tttgaagatc aggcacccag cattcgtctg cgggataacc aaccggtatt agcagaaaag
    19261 gtttttcatt ttcgggcctg tctaaaatct tacttaaaaa attcatgggc gatggagtat
    19321 gtgtcaatgc agcaagccct gcatgatgta tagctgccaa taaaaaaccg caggcaatac
    19381 cgcaactttc ggtaacataa tagttagggt gcttatggcc ttcatcttcc agttcgtagc
    19441 ttcttttaaa aattattatc aggcacggag ctgtttccag gaattctttt ctccaatctg
    19501 tttgtaaggg tctaaggtcg ttcagccatt cttcaggcat tcggccatta tagctttcta
    19561 gttcttcttt ctctgcttct tgcctgattc gcttctttaa ggttggttcg cttacaacac
    19621 aaaatgtcca gggttgttta tgggcaccgc ttggagcagt tgatgcggtt agaattatat
    19681 tttctattac tttttggggc actggtttac ttgagaattc ccgtactgtt ctgcgttggt
    19741 tcatccattg gcaaaattct ttgctgcgtt gcatcatatc tttagtagga aacgtctgtt
    19801 ttgcataagt gataaaagga taaccatcga tcaattttgt ttttaccata acaagtattt
    19861 ggcgaaattt accaattcaa attgtattag atgaagaaat taattgtatt atctgtaacc
    19921 ctttttcttt ttgtttttag cacttctgca caacccgacc gctggcagca acgggtaaag
    19981 tatgtgatgg acattgatat gaatgtgctg accaaccagt ttacaggcaa acagaaactg
    20041 gaatattgga ataattcacc tgacaccctg accaaacttt tctatcatct ttattttaat
    20101 gcttttcaac ccggcagtat gatggatacc cggagnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn ncaagagtga gggatagaat cattaattta aaaccggatg aaatcggtta
    20221 ccagaaaatc atttcattaa gaatgaatgg tcgtccgcaa ttattcaaag tgctggagac
    20281 aattctggaa gtaaaattag ataagccgat cctgcctcgg tcaaaagtca cattagatat
    20341 ggagtttgag gcacaggtac cgttacaggt tcgccgcagc ggaagggata atcccacctc
    20401 caaagtccgt tattctatga gccagtggta tcctaagtta tgtgaatatg actatgaagg
    20461 ctggcaccct acaccctatg tggcaagaga attttatgga gtctggggag attatgaagt
    20521 gaatattact attgataaga aatacatttt gggaggaaca ggttatctgc agaatgccaa
    20581 ccagattggc tatggatacg aaaatccggg tgttaaagtt aaccggcccc ctggtgataa
    20641 gttgacctgg aaatttgttg ctccgaatgt ccatgatttt atgtgggcag ctgatccgga
    20701 gtacaaacat gtttccagaa aaataaggga cagtcttaca attcatgtgt tgtataaaaa
    20761 caccaacgaa ccggcaaaag attgggaaga cctgctgaat atggcagaaa gggcattacc
    20821 ctatatcgaa aagacatttg gtgtatatcc ctataaacaa ttttcattta ttcatggtgg
    20881 cgatggtggt atggaatatc caatggcaac attatgtatt ggcccaggtg ctgcattgca
    20941 tgaatggctg catagctggt attatggttt actgggcact aacgaaggat tatatccctg
    21001 gatggacgag ggttttaccc agtatgctga ggaaagagta aatgcatgga tgagcaacag
    21061 ctccggattt gcacaggccg gtaactacgc agcgtattat gcgttggtga aaagtggtaa
    21121 agaggagcct atgtcaactc acgccgatca ttataacacc aactttgctt actccattgc
    21181 ttcttattca aagggaacag tatttattga acagctggga tacattactg gtgcagaaac
    21241 gagagacagg atattactgg aatattataa acaatggcgg ttcaagcatc ccaatgtgaa
    21301 tgattttatc cgtattgctg agaaagtcag tgatatgaag ctggattggt acaaggaata
    21361 ctgggtaaac tccaccaaga cgattgacta cagtatcgac agcttgtggg aggaaggcgg
    21421 aaaaacaaaa gttcggttaa accggatagg tcagatgcca atgccaattg acctccagtt
    21481 aacttttaaa gacggttcaa aagaaataca ttatgtgccg atgaacctca tgtatggtga
    21541 aaaaccgggt gaagattcaa ccataaccag aaaagtatac gaaccctgga gatggacaca
    21601 cagtacttat acgattgaaa caaaccggaa actggcagac ttctcgctgg tagaaattga
    21661 cccttcgctt cgaatggccg atgtagaacg aagaaataat agactggaac ttaaatggta
    21721 attatttaaa gcaaaaccct ccgctgatta tctgcggagg gttttttgac ggtttgaatc
    21781 aaggtccctg tatgcttaat agagtaaggt ttaaggctaa cggtcgtatc taatagctta
    21841 attatctgag tcaaaaatag aaggggtatg cataccagac aataccacat tagtggtatt
    21901 ttttgaagaa aaatgaattt ttgcctgcaa aaacctgcaa aaattacctc ccataatatt
    21961 tattgacggc agctaccctg ttatttacat gtaatttttc gtaaatatgg taaacatgtt
    22021 tccggaccgt ttccgggcta aggaagagtt gtgaagcaat ttctttatac attaaacctt
    22081 ttgaaaggaa ttccagaatt tctttttccc ggttggaaag catatctagc ctttcagatt
    22141 ctgcctcgac agtagcagat tgctgttgct gaaaaacagc caccactttt ctagcaatct
    22201 ggctgctcat aggagcaccc ccttcataca attcccggat ggcttcaagc attcggccgg
    22261 gatctgtttt ctttaatata tatccgctgg cgcctgcact cagggcttca aagatctttt
    22321 cattttcctc gtaaactgtg cacatcataa aatttgtgtc cgggatgcgg ggttttagta
    22381 ctctcacaat atcaattccg ctttcttctg tgcccaggtt aatatccatc agtactacgt
    22441 ctggcctcag gataggtaat tggttcattg catcttccac tgtacctatt gtgccaaggc
    22501 acctgtatcc ttcagacatg ctgataattt cttcaagagc atttctcagc tctttattat
    22561 catcaacgat acatacggag atttccttca tgcaagtaaa attctgtttt aataaggtgg
    22621 aaaacaatac cacaaagtgg taattttttt atacaggtac aaataaaagg tacttttctt
    22681 tgaagaactc ttccggaaaa atatcattga tttccattat tctcggtttc gtaccgctat
    22741 catgaatttc ggcagcaagg tcgcctcctt tcaggcaaat aagtcctgac ttttgcccag
    22801 cgggcatttt tgttgaatct ttttttaaaa ttggcctgct ccatttccat aagtctttga
    22861 gaggcgccac agcccgggat acaacaaagt caaacttcct gtttttgata ttttcaatcc
    22921 ggctatgctc ggtagttata tttttaagcc caatgccacc ggcaacagcc tctactactt
    22981 ttagcttttt ggcaatactg tctaccaggt gaaattttac ttcgggaaaa aaaatagcca
    23041 ggggtattcc cggaaaacca cccccagtcc ccagatcgat gatctccgaa ccgtcagcaa
    23101 aatcaaatgc tgctgctata cttaatgagt gcaaaacatg tttttcgtaa aggctgtcaa
    23161 tatcctttcg ggaaatgaca ttgatctttt cattccattc tttatataat ccttccagtt
    23221 tactgaattg cccaagctga gcaggagtaa aatcggagaa gtattttaaa ataatatcca
    23281 tgattatact gtcagctcca gcctttggcg ggtttgcgcc atagtgccgg ggcaaatatg
    23341 agataataaa aaaacatcca catgtcaagg aaaataaaat aaggccagag gtccttttcc
    23401 cccatttttt tcattgactt ataaaaaata attgcctgta ataataaacg aatgccaaat
    23461 accgccaggg catatcgcca gtcgaaaaag ataatagcag cggccannnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23641 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gatctataac cactgcggta tttgatttag
    23701 tagccgtttt attaatgaac aaatcatcat cgccgctggg tatatgattg atggaggaga
    23761 agcctttgtg tttgaagaaa aggcttttcc ggtaagaaag atttctgcct actcccatat
    23821 agggtatgcc tgcaagggca taagacaggt attgcaaagc ggtatgaaat gtttcaaacc
    23881 ggatgagttt gttcagcaag cctcttcgtt tgtgat
//
LOCUS       DAORLS010000013        23902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1171_length_23902_cov_2.203925, whole genome shotgun sequence.
ACCESSION   DAORLS010000013 DAORLS010000000
VERSION     DAORLS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 23902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23902
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_23902_cov_2.203925"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PK977_02235"
     CDS             <1..1160
                     /locus_tag="PK977_02235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRF16951.1"
                     /translation="NAGISTASQLLRKNKNLDIAIIEPSDKHYYQPAWTLVGAGVFNM
                     KNTIHNEADVMPKSVKWIKQKVVTFQPEANSVTLDNNETVTYDYLIVAPGIQLNWGDI
                     KGLKETLGKNGVCSNYSVDNVPYTWECLKNFKGGKALFTNPNTPVKCGGAPHKIMWLA
                     ADYFKKHGVFDKTEIQYWSGGTRLFGVEKYEKTLKKVAERDKVQMTFYTKLVEIDGPN
                     KKAKFVGFGEQNKDKEYWVSFDMIHITPPQSAPDFVKNSPLANAAGWVDVDKFTLQHV
                     KYPNVFSLGDASSLPTSRTGAAIRKQAPVLVKNLLAFMEGKQLTGSYNGYTSCPVVTG
                     YGKLVLCEFDYNNKPIETFPIDQSKERWTMYQLKRRVLPWLYWHKILPGKM"
     gene            1272..1526
                     /locus_tag="PK977_02240"
     CDS             1272..1526
                     /locus_tag="PK977_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16952.1"
                     /translation="MTLIDRLKSGWTVLKFIRVTLSILILSSSIGSGEVSGIILGTVF
                     TVFSLFTDGVCCAGGSCYTPVKKNNSATPENIEYEELGSK"
     gene            1504..1716
                     /locus_tag="PK977_02245"
     CDS             1504..1716
                     /locus_tag="PK977_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HRF16953.1"
                     /translation="MKSWVLNNKLLIAGIIIGAAGGYLYWQQIGCSSGTCAITSKWHN
                     STAYGALMGGLLFSMFKREKNDNNKQ"
     gene            1697..2023
                     /gene="trxA"
                     /locus_tag="PK977_02250"
     CDS             1697..2023
                     /gene="trxA"
                     /locus_tag="PK977_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRF16954.1"
                     /translation="MTTTSNKRETFGEIINGSTPVLVDFFAEWCGPCKLMKPVLEELH
                     ARLGDKVRILKIDVDKSPSVAGALQIQSVPTLMLFQHGKTLWRKSGVMQATQLEKIIE
                     QHSINS"
     gene            2026..2481
                     /locus_tag="PK977_02255"
     CDS             2026..2481
                     /locus_tag="PK977_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRF16955.1"
                     /translation="MSDKKKHRGYLSSAITCHCPRCREGKLFKHPVTIGKRNLEMHKN
                     CAVCGQPTEIEVGFYYGTGYVSYALGVAFTVSTLVAWYVLIGMSTSDNRFFYWLGINS
                     VLLIMIQPWLMRLSRSLWISWFVKFDPNWEENQPVDVSERLNPDQANNW"
     gene            2520..2607
                     /locus_tag="PK977_02260"
     tRNA            2520..2607
                     /locus_tag="PK977_02260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2554..2556,aa:Ser,seq:gga)
     gene            2760..3146
                     /locus_tag="PK977_02265"
     CDS             2760..3146
                     /locus_tag="PK977_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16956.1"
                     /translation="MKKIALFAIFLWMVSGKGVAHATSTGPMSYPVYDETWMKMKAVD
                     FIKLKVADFAKICNKKLTLKEKVSFLVLKKNLKRELKKNPDLTVAGYIASGKKGISTI
                     AWIGIIAGVILITLIIISTASDGWLL"
     gene            complement(3242..>3662)
                     /locus_tag="PK977_02270"
     CDS             complement(3242..>3662)
                     /locus_tag="PK977_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRF16957.1"
                     /translation="IHSRESVDECIRVVSEHQKGKLKGVFHCFSGNHGQAQQIIDLGF
                     YLGIGGVVTFKNSGLDKVMEKVSLEYVVLETDAPYLSPVPFRGKRNEPAYLKYVVEKL
                     CVIKKTTITEINAVTTSNVQKLFAKAFSENHLLKTPD"
     assembly_gap    3663..3672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3673..4051)
                     /locus_tag="PK977_02275"
     CDS             complement(<3673..4051)
                     /locus_tag="PK977_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRF16958.1"
                     /translation="MNLIDTHSHIYLSEFETDRETMLQRAEKEGIAKILMPAIDTETH
                     ENMLKLEQKHPGICFAMMGVHPCSVKSNYKEELKAARAYLENRPFIAIGEIGLDFYWD
                     KTFVAEQYLAFHEQIEWALEFGIP"
     gene            4168..>4885
                     /locus_tag="PK977_02280"
     CDS             4168..>4885
                     /locus_tag="PK977_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16959.1"
                     /translation="MKSRIISFPLAAIFFFFAVLSITSCQKEQSQNGSEEEQEMEASR
                     VSSEADGESEIVFNGVFDDVMGVNDEVGVGGTGIFGRVTSCPDVIIDRLNPPSPFPVR
                     ITLDFGTGCVGRDGHFRKGKIVTVYTGRLIVPGSVATTEFVDFYFDSVRVQGVHKITN
                     TSSPVANIPPARQFKAEVINGKLTRPNGNFTEWNSVKVITQIEGLATPIPLDDIFKIE
                     GSARGRVKRGNLLTIWESNII"
     assembly_gap    4886..4896
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5178..>5639)
                     /locus_tag="PK977_02285"
     CDS             complement(5178..>5639)
                     /locus_tag="PK977_02285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF16960.1"
                     /translation="YNAKATFFCIGKNVESNPGLYDRIIAEGHQTGNHTQNHLNGWKT
                     ADEVYLDDVRLAARSIQSALFRPPYGRIRSRQVKKLSVYKIVMWDVLSGDFDLRISKE
                     TCLHNVLNNTGSGSIVVFHDSEKAWDKLQYSLPQVLEHFSKKEYKFLSLTL"
     assembly_gap    5640..5743
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5862..>5977
                     /locus_tag="PK977_02290"
     CDS             5862..>5977
                     /locus_tag="PK977_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF16961.1"
                     /translation="MSKKVRVRFAPSPTGGLHLGGVRTVLYNYLFAKKNKGD"
     assembly_gap    5978..6059
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6060..>6383
                     /locus_tag="PK977_02295"
     CDS             <6060..>6383
                     /locus_tag="PK977_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF16962.1"
                     /translation="WCGLEPDESVQHGGSYGPYRQSERKEIYRQYAEKLVAEGHAYYA
                     FDTPEELEKMRQDFKTAQNPSPQYDHTIRMRMRNSLTLSTEKTKELLDAGTRQVIRIK
                     MPEKET"
     assembly_gap    6384..6437
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6438..>6820
                     /locus_tag="PK977_02300"
     CDS             <6438..>6820
                     /locus_tag="PK977_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF16963.1"
                     /translation="TVDDKVLLKADGMPTYHLAVVVDDHLMQISHAFRGEEWLPSAPV
                     HILLWKYLFGLENMPQWAHFPLILGPNGKLSKRDGAKYGFPVFAMNWTDPKTGELTEG
                     FKEKGFLPEAFINLLALLGWNDGTE"
     assembly_gap    6821..6880
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6881..7386
                     /locus_tag="PK977_02305"
     CDS             <6881..7386
                     /locus_tag="PK977_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRF16964.1"
                     /translation="FDYEKAKWFNHEWIKRMKVEDLNPKVSELLVQSGITITNKDLLD
                     KIITLVKDRCTLLTDFIQQSTFFFKAPEEVDVDAVKPKWSEAKKLFFAELIRNYELAP
                     DWNAHTLETNFKEIASVNDLKPGELMLPFRIMLVGGKFGPGVFEIAELLGKEETIKRM
                     QFAISKF"
     gene            complement(7383..8372)
                     /locus_tag="PK977_02310"
     CDS             complement(7383..8372)
                     /locus_tag="PK977_02310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF16965.1"
                     /translation="MQYRKFGATELLVSEVGFGAWAIGGGAMVGTTAIGWGNADNEVS
                     EKAIYASLDAGINFFDTADIYGLGHSEELLGKTIGGIKDVIIASKAGNVSRNNEFTVD
                     YSRQHIKSACEASLKRLKREVIDYYQLHTARQSHLKEGDCIEAMQQLQQEGKIRYWGL
                     SLNTFYPLTEAEYLLQQELGNGFQLVLNLLNQNALPLLKKAVEKGYGIIARMPLQFGL
                     LTGKFDKEAEFPENDHRKNRLTPEVVSVCSDALAPVWELCEKYNCTKTQLALSYVLSY
                     SEVSVIIPGIRTSKHVAENTTGLFQLDMADREMIEKRGCNDFLQVMELIRLQG"
     gene            8431..8838
                     /locus_tag="PK977_02315"
     CDS             8431..8838
                     /locus_tag="PK977_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRF16966.1"
                     /translation="MKRPIRVLVAKVGLDGHDRGAKVIATALRDAGMEVIYTGLRQTP
                     EMVVNAALQEDVDAIGISILSGAHNTVFPKVINLMKEKGMDDVLLTGGGIIPDEDIEE
                     LNKIGVGKLFHPGTNTHEIADYISNWVKEHRNF"
     gene            8851..9627
                     /locus_tag="PK977_02320"
     CDS             8851..9627
                     /locus_tag="PK977_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRF16967.1"
                     /translation="MYQTLLASLENGIYTITINRPDKLNALNKDVFTDLNTALDEIES
                     NPEIKSVIITGSGPKAFVAGADISEFNSLNKEQAKALAKRGQDIFARIENSSKPIVAA
                     VNGFALGGGCELAMSCHFRVASDNAKFGQPEVNLGLIPGYGGTQRLVQLIGKGKAMEL
                     LMSAHMIDAKEAKELGLVNYVTTTDTLLEHTRKILDVINSKAPLAVAGCIKAANAVFN
                     ESIDGFALEIEEFGNTFDTEDMKEGVTAFLEKRKADFKGK"
     gene            complement(9633..11051)
                     /locus_tag="PK977_02325"
     CDS             complement(9633..11051)
                     /locus_tag="PK977_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRF16968.1"
                     /translation="MRIFFLFFILFVSTFVSAQKTEISPLPTKAQLAWHENEFYLFMH
                     FGPNTFTGKEWGEGNEPEDIFNPTELDCRQWCRIARAAGAKGIIITAKHHDGFCLWPS
                     KYSKHTVRESKWKDGKGDVLKELSQACREFGLKFGVYISPWDRNHPDYGTDKYNDVFV
                     GMMREIFQNYGPVWELWWDGANGEGPNGKRQEYDWRRYENTVRQLSPKTVVFSDIGPD
                     IRWVGNENGFVGDPNWNFLDTVGYKRGIGAPANDTLNHGNYYGKHWIPAECDVSIRPG
                     WFYREAEDNKVKSAGKLFDIYLKSVGRGANLLLNIPPDKRGLIHEADSAELMQFRKMR
                     DENFATNLLKDADSYYEFSQKDLYNKPLLLRGFDTTSAYYGINLQNFIVQLSQPVKTN
                     CIVLREAIHLGQSIRRFSIVLYSNDKPIGEVQGTSVGRKRILTFPATTITSFRVYLED
                     AQGNDNITGIAAYLIDESLVEQ"
     gene            complement(11048..12142)
                     /locus_tag="PK977_02330"
     CDS             complement(11048..12142)
                     /locus_tag="PK977_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF16969.1"
                     /translation="MKSVCFLFLLLLSIGGSSQQQTRNIIIITTDGLRWQEVFQGMDT
                     SIANKSKYNEGDSTYIYSSYWSADERERRKKLMPFLWSTIVSKGQVFGNRRLGNKVDN
                     ANPYWFSYPGYSEIFTGYADTAINSNNYPPNPHVSVLEFLNNQPPLKGKVAAFGAWNA
                     FDRILNEERSKIPVVCGFDITGDKSPTANEKLINQMLSDSYKPWLEDECLDIFTHYAA
                     MEWLKTRKPRVLYIGYGETDEWAHAGRYRSYLDAARQVDAWIKEIWEFVQKDPQYKDK
                     TTLFITTDHGRGDIKKEEWTSHGSSIEGANEIWFAVLGPDTPERGEINTSVLLYQKQF
                     AQTMAKLLGYTYKATHPIADAILYEFKMKK"
     gene            12236..13639
                     /gene="fumC"
                     /locus_tag="PK977_02335"
     CDS             12236..13639
                     /gene="fumC"
                     /locus_tag="PK977_02335"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRF16970.1"
                     /translation="MEYRIEKDTMGEVKVPANAYYGAQTQRSIDNFKIAQDINRMPKE
                     IIQAFPYLKKAAAITNLEAGVLPKNKATLIGKVCDEILKGKLDDYFPLVVWQTGSGTQ
                     SNMNVNEVIAYRGHVLKGGNLIDKEKFLHPNDDVNKSQSSNDTFPTAMHIAAYKILVE
                     TTIPGIKKLRDTLAKKSKQYMHVVKIGRTHFMDATPLTLGQEFSGYVSQLDHGLKAIK
                     NSLSHLSELALGGTAVGTGINTPPNYAENVAKHIAKLTGLPFVTAENKFEALAAHDAI
                     VEAHGALKTVAVSLMKIANDIRMLSSGPRSGIGEIFIPDNEPGSSIMPGKVNPTQCEA
                     LTMIAAQVMGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGDGCVSFNDKCAVG
                     IKPIEANIKKHVDNSLMLVTSLNTKIGYYKAAEIAQKAHKEGTTLKEMAVRLGYVTPE
                     QFDQWVVPANMVGKLEK"
     gene            13786..14046
                     /locus_tag="PK977_02340"
     CDS             13786..14046
                     /locus_tag="PK977_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16971.1"
                     /translation="MKNAAHPSLFFLANPLKAVQAEPAAAAEDNYQTPYLRSILSAKS
                     APSRVYSLRSIREKFVSTVRPFISTLPKDVELSEKNWFNNYE"
     gene            14319..16613
                     /locus_tag="PK977_02345"
     CDS             14319..16613
                     /locus_tag="PK977_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF16972.1"
                     /translation="MKRGVPNTFYRIFLTVTIIILILTVSSGYIHAQTVDFGKSYINV
                     TKGLNGGTLETGDTLEIRASFVVRSGTYDSCRYRDNVPTGTTFIPGTIRVLTNEGKIY
                     KQLTDGYGDDPGWITGTQVTIHLGFNPSAAPATAFRRGRVANTHKPSFYSSTCIMIAS
                     FRVVVTAVTGSNISTGGGTITYKSGSNPIQTYTFPSNTVRVYPNYGICSNTIGTNAIG
                     TEFNGTFGSGQPRNRGTSANVPTGYTYNIFNTNSPQDYYYGIANNTSTRVNYTTLNTW
                     AKPDNSSPTHRVFNVWDIIGDHTGATDQYSGNPPADTVANPNAGYMLVINAAYRIDSA
                     FQQTITGLCPNTFYEISCWMRNICSKCGCDSNGRGATSSAGPPYYIPTATGDSSGVRP
                     NITFEIDGVDYYTTGDLLYTGQWVKKGFTFLTGPAQTSFTLKYFNNAPGGGGNDWALD
                     DITVATCSPDMKYGPMNNPIVCDSNVLVLSDTIRSYFNNYTEYKWQRSTDGGATWNDI
                     PGATGSGVPVWNGTAWEYVAYYTIPISQTQMSNNGDMYRLVVATTVANLSNDNCSFSD
                     ALTITVSVIECGIPLHARLLSFSGILNSQQRTDLQWITTGENEPVLFDIEKSFNGSNY
                     TVIGTINGHNDYSSEYSYYSFTDSAPVSGKVYYRIRMRNNTQSVYSRTIQVSNIADHC
                     SFVSVINPFKHKLDFELSAATQERAEILLIDQSGRIVKQKVILLSNGINNLSIEDTEK
                     LQPGIYYLRVAAGGTVLQKKVLKQ"
     gene            complement(16629..17042)
                     /locus_tag="PK977_02350"
     CDS             complement(16629..17042)
                     /locus_tag="PK977_02350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRF16973.1"
                     /translation="MCRLIYAGALGNLIDSMFYGMIFDKGLHYDAAINEYVTYSGIAE
                     FSSSGYSSFLHGSVVDMLYFPIIKSNFPSWMPFVGGQDFEFFSPIFNIADASISIGVI
                     TLLIFQKRFFKKHMADDAHSTVETNTDVSDKVQVS"
     gene            complement(17030..>17404)
                     /locus_tag="PK977_02355"
     CDS             complement(17030..>17404)
                     /locus_tag="PK977_02355"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRF16974.1"
                     /translation="YYDFFHRFALSAHLNLLIFVPIMKTLKLRHVILLISIIIIVDQA
                     LKIWIKTSHPTGEVLRVFGMDWFRIHFIENPGMAWGWKFGNETGKMILTLFRLAAVIF
                     GTWYLGKIVKQQYSRGFVVCAG"
     assembly_gap    17405..17427
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17428..>18391)
                     /gene="alr"
                     /locus_tag="PK977_02360"
     CDS             complement(<17428..>18391)
                     /gene="alr"
                     /locus_tag="PK977_02360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRF16975.1"
                     /translation="GILQYHKVDYLGVAYADEGVELRKAGITLPIMVMNPEENAFDAI
                     VEYDLQPEIYSFELLQSFDYFLQQQGLQQYPVHLEIETGMNRLGFVPGDIGKLADYLL
                     LCPSFRVETVFSHFAASEEKANDDFTLTQYSVFNNAMSELEEKLGYSFLKHIANSAAA
                     VRHPGLQLDMVRLGIGLYGVDSAASGLLNLQTVATLKSTIAQLKRLQEGDSVSYNRKG
                     KIEKESLIATIRMGYADGFPRSLGNGVGKVLVRGKLVPVIGTVCMDMFMADVTHVPGV
                     KEGDDVLIFGESLPVQSVAQWAETIPYEIMTGISQRGKRVYFEE"
     assembly_gap    18392..18418
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18419..19795)
                     /locus_tag="PK977_02365"
     CDS             complement(<18419..19795)
                     /locus_tag="PK977_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF16976.1"
                     /translation="MTYTIHHIAQIIKADAVIIHDSVIENLLLDSRKVFAPASSLFFA
                     LKGPRRDGHQFIADLYKRGVMNFVVDRQMEIKEYPEANFLRVNDSLEALQLLAAHHRR
                     QFSIPVIGITGSNGKTIVKEWLYQLLHQDYTIVRSPKSYNSQIGVPLSVWQMNKQHTL
                     AIFEAGISRPGEMEKLQKIIQPTIGVLTNIGEAHSEGFSSLQEKETEKKKLFKYAVLP
                     EQLRVKEIKRQNDYTIITAGQGHQLQIPFTDDASVQNAITCWQVMLHLGYRQEVVQER
                     MLLLTPVNMRLELKKGINHCSIINDSYSADISSLEIALNFLGQQAGSHKKTVILSDFL
                     QSSIADELLYRQVMESLVKHKVNRLIGIGERVTKQINYHFPQGYSDIDIILFRTTDEF
                     IHQFRSSQFKEEVILVKGARIFAFEKIVQLLEQKVHQTVLEINLSAIVHNLKEYQRRL
                     KPTTKIMAM"
     gene            complement(19804..>20182)
                     /locus_tag="PK977_02370"
     CDS             complement(19804..>20182)
                     /locus_tag="PK977_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tellurium resistance protein TerC"
                     /protein_id="HRF16977.1"
                     /translation="IMLAAIDLVFALDSIPAVMGISRDKLVIYTSNIFAVLGLRSLFF
                     LLRGAVSKFDYLQQGIAIVLVFIGCKMLGEHWISEWVSKQIQVIISLGVIMVCISGSI
                     FYSIFMQKKGEPEDVTDESQSPG"
     assembly_gap    20183..20192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20193..20785)
                     /locus_tag="PK977_02375"
     CDS             complement(<20193..20785)
                     /locus_tag="PK977_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerC"
                     /protein_id="HRF16978.1"
                     /translation="MTKEQVEYLVFGIVLVLALVFDLGLLSKKDQKITIRQALYQTFF
                     WVGLALAFFVFMWVEEGERLALEYLSGYLMEWSLSIDNIFVFILIFSAFSVKEKYYGR
                     VLLIGILMAIIFRVIFITVGVALVEKFHWILYVFGVFLVFTGYKMFTASDDEEFNPQE
                     SKIYRFMKKFLPLVPHDGNGKYRVIVNGKPAYTTLFVV"
     gene            complement(20865..22604)
                     /gene="gldG"
                     /locus_tag="PK977_02380"
     CDS             complement(20865..22604)
                     /gene="gldG"
                     /locus_tag="PK977_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRF16979.1"
                     /translation="MKIINKIFASKAWWFWLLLLLFIINYAASVFHARFDLTKEKRYT
                     LSKATKDLLKNLEDNVQIDVFLKGQFPAGFKKLANSTNEFLQLMKDRNGSKIHYRFIS
                     PQEEIADTPGKTYEDTLIGLGANAINLTVQVAEGQENKRVYPVALMRYKGKQALVNLY
                     SGGKRMITPVEMNSAEALMEYQFAKALDGLITTEKPHVAYSIGNGEPVDANTYGIQDA
                     LKNDYQLGIFDLNKHSKISTDINDKTRIDVFMIVKPTLQFTEEEKLKIDQYLMRGGKL
                     LMFIDNLIAEQDSLSAVKETVAFDRNLNLTDQLFRYGVRINTDLVMDLQCDYMPFVVG
                     GDLQNPQFEFLKWNYYPLFESKGNHTINKNIGLVSGRFVNSVDTIATPGVNKTVLLSS
                     SADSRIISTPALISLNENKNVPKDIKFNKSDIPVAMLLEGKFTSLYRNRASRSQLDSL
                     TATGSSFLAEGNGIGKVIVVADGDMVLNDVSPKEGPLPMGINFYTVGSQFEYQFANRE
                     FLLNCLEYLVNKPGIIETRNKDIVLRLLDGQKVKEQKTTWQFINIALPVALVILFGWL
                     YQGIRKRKYAGGF"
     gene            complement(22608..>23313)
                     /gene="gldF"
                     /locus_tag="PK977_02385"
     CDS             complement(22608..>23313)
                     /gene="gldF"
                     /locus_tag="PK977_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRF16980.1"
                     /translation="KKELRQFFSSLTGYIAIIVFLLVNGLVLFVFEDSILDFGYATLD
                     RFFQLAPWILLLLIPAITMRSFAEEFKTGTFEILQTRPLSRWQVVGGKYLGSLIVVFI
                     ALLPTVVYIFTIQQLSSNQGLDIGATIGSYIGLFFLAAVFTSISICSSSFTNNAVVAF
                     IISLIGCALLYYGFSAISKLPALANGPDYYVEMLGIDFHYRSISRGLIDTRDIMYFLS
                     VIFLFLTITNRNLLKR"
     assembly_gap    23314..23370
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23480..23833)
                     /locus_tag="PK977_02390"
     CDS             complement(23480..23833)
                     /locus_tag="PK977_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRF16981.1"
                     /translation="MSVSLDNAIYVSDKAKAKVKQLLADAGVADDPSYFLRVSVVGGG
                     CSGLSYKLDFDNEQKPMDQVFEDNGVKVVTDLKSFLYLVNTTLDFSDGLNGKGFYFSN
                     PNASRTCGCGESFAV"
BASE COUNT         7146 a         5045 c         4546 g         6727 t
ORIGIN      
        1 gtaatgccgg tatctccacc gcttctcaac tattacgtaa aaacaaaaat cttgatattg
       61 ctatcatcga accatctgac aagcattact atcagccagc atggacattg gttggagctg
      121 gtgttttcaa tatgaaaaat accattcaca atgaagccga cgtaatgccc aaatcagtaa
      181 aatggatcaa acagaaagta gtaacctttc agcctgaagc caatagtgta accttggata
      241 ataatgaaac tgttacctat gattacctga ttgtggctcc gggcatccaa ctgaattggg
      301 gagatataaa aggtctgaaa gaaacattag gtaaaaatgg cgtatgctct aactatagtg
      361 tagacaatgt gccttatacc tgggaatgcc tgaaaaactt caaaggaggc aaagctttat
      421 ttactaatcc taacacacct gtaaaatgtg gcggtgcgcc ccacaaaata atgtggctgg
      481 ctgctgacta ctttaaaaaa catggtgtat ttgacaaaac agagatacag tattggagtg
      541 gtggcacaag gttatttggt gtagagaaat atgaaaagac cctgaagaaa gtagccgaaa
      601 gagataaagt acagatgaca ttctacacta aattggtaga aattgacggc cccaataaaa
      661 aagcaaaatt tgtaggcttc ggagaacaaa ataaggacaa ggagtattgg gtaagctttg
      721 atatgataca tattactccc ccgcaaagtg cccccgattt tgtaaagaat agtccattag
      781 ccaatgctgc tggctgggta gatgttgaca aattcacttt acagcatgta aaatatccta
      841 atgtattctc actcggtgat gcttcttcat taccaacatc cagaacagga gcagcaattc
      901 gtaaacaagc cccagtattg gtcaagaacc tgcttgcttt tatggaaggc aaacagctga
      961 ccgggagtta taatggctat acaagttgcc ctgtagtcac cggttatggc aaactagtac
     1021 tttgtgaatt tgattataac aacaaaccga ttgaaacatt ccctattgac cagagtaaag
     1081 aacgctggac gatgtaccag ttgaaaagaa gagttttacc gtggttatac tggcacaaga
     1141 tattacccgg taaaatgtag acattgatgt aaggtttgct aagcggctgt ctttgacagc
     1201 cgtttttatt tgtaacaaat gtcactacag ccttcacttc tgcatctgaa atttgtcttc
     1261 aaatcaataa aatgactctt atagacaggt taaaaagcgg atggacagtc cttaaattca
     1321 tccgggtaac gttgagtatc ttaattttgt catcctctat cggctctggt gaagtatcag
     1381 gaataatact gggaaccgtt tttacagttt tttcgttatt tactgatgga gtttgctgtg
     1441 ccgggggaag ctgttatact cctgtaaaga aaaataattc tgccacacct gaaaacattg
     1501 aatatgaaga gctgggttct aaataataaa ttattgatcg ctggtattat aattggcgct
     1561 gccggaggat acttgtactg gcagcaaata ggctgcagca gtggtacttg tgccattact
     1621 tctaagtggc acaacagtac ggcctatgga gcattgatgg ggggattact attcagtatg
     1681 tttaaaagag aaaaaaatga caacaacaag caataaaaga gaaacgtttg gggagatcat
     1741 caacggaagc acaccagtgc tggttgattt ttttgctgaa tggtgtggcc cctgcaaact
     1801 gatgaaacct gtacttgaag aactgcatgc cagactggga gacaaagtcc gcattcttaa
     1861 gattgatgtg gacaaaagcc cttctgttgc cggtgcttta cagattcaaa gtgtacccac
     1921 attaatgcta tttcaacatg gaaaaacatt atggcggaaa tcaggagtta tgcaggctac
     1981 acaattagaa aaaatcattg aacagcattc gatcaactca tagacatgtc tgacaaaaag
     2041 aaacacagag gttatctctc atctgctatt acctgccatt gccctcgttg ccgggaagga
     2101 aaactattta aacatcccgt aacaattggc aaacgaaacc tggaaatgca taagaattgt
     2161 gccgtatgcg gccagcctac tgagatagaa gttgggtttt attatggaac cggctatgtc
     2221 agttatgcat taggcgtggc ttttactgtg tcaaccctag ttgcctggta tgttttaatc
     2281 ggcatgtcca cttcggataa ccgttttttt tactggctgg gcattaattc tgttttactg
     2341 atcatgatac agccctggct gatgcgcctt agccgcagtc tatggatatc ctggtttgta
     2401 aaatttgatc ccaactggga agaaaatcaa cctgtagatg tatcagaaag attaaaccct
     2461 gatcaggcta acaactggta agtcaataat ccttattttt gccgcccttc acaactaatg
     2521 gagacctgct cgagtggttg aagaggcacg cctggaaagt gtgtataccc ccaaagggta
     2581 tcgagggttc gaatccctcg gtctccgctg tagcccgcca acaacttaac tgttggcggg
     2641 cctttttttt catcttagat ttttattatc attttcccta aatgatattt ctcattggat
     2701 tcagcccgat caagagttta acttaatagc acaattttat tactataaaa ctatgttgta
     2761 tgaaaaagat agctctgttt gccatctttt tatggatggt tagcggaaaa ggtgtggccc
     2821 atgcaacaag tactggacca atgtcatatc cggtttacga tgagacgtgg atgaaaatga
     2881 aggcagttga ttttatcaaa ctaaaagttg cagacttcgc caaaatttgc aataaaaaat
     2941 tgactcttaa ggagaaggta tcattcttag tgctaaagaa aaatctgaaa cgggaactta
     3001 aaaaaaatcc agatctgact gtggccggtt acattgcttc cgggaaaaaa gggattagta
     3061 ctattgcctg gataggaatt atagctggtg ttatactaat cactttgatc attatttcta
     3121 ccgcaagcga cggatggtta ctgtaacctg tattgtgaat aattatttcc ttgatacgta
     3181 cgctgactac agactcagtg aagataaact tttgtcaacc tgcattctta tcccgtaact
     3241 atcaatcagg agtctttaac aggtggttct cggaaaatgc tttagcaaaa agtttttgca
     3301 catttgaagt ggtgactgca ttaatttctg ttattgtagt tttcttaata acacaaagtt
     3361 tttccactac atatttcaga tatgcgggtt catttctctt tcccctaaac ggcaccggcg
     3421 acaggtaagg agcatctgtt tctaaaacaa catattccaa tgaaaccttt tccatcactt
     3481 tatctaaacc tgaattctta aaagtaacaa ccccgccaat gccgaggtaa aagccaagat
     3541 caattatttg ttgcgcctgt ccgtgattgc ccgaaaaaca atggaatacg cctttcaatt
     3601 ttcctttctg gtgttcggaa accacacgga tacattcatc aacagattcc cttgaatgaa
     3661 tannnnnnnn nntgggtata ccaaactcca gggcccattc tatttgttca tggaaagcaa
     3721 ggtattgctc tgcaacaaag gttttatccc aataaaagtc cagaccaatc tcgccaatgg
     3781 caataaaagg tctgttctcc agataggccc gggcggcttt taattcttcc ttgtaattac
     3841 ttttaaccga acaaggatgt acacccatca ttgcaaaaca gatcccggga tgcttttgct
     3901 ccagtttcaa catgttctca tgggtttccg tatcaatggc cggcattaat atttttgcaa
     3961 tcccttcttt ttcagcccgc tgcaacattg tttccctatc ggtctcaaat tcagacaggt
     4021 aaatgtggga gtgtgtatct atcaggttca ttatcaggga tctttgggtg acaaaactca
     4081 gcaaaaaact ttaagattcc tttaaacttt ggtcaataaa tcaattctta caggcgttaa
     4141 actaacttaa ctacaaaact atagtttatg aaatcaagga tcatttcctt cccgctggca
     4201 gctattttct tcttctttgc agttttgtcc attacatctt gtcaaaaaga acaaagccaa
     4261 aatggctctg aagaagaaca ggaaatggaa gcttcaaggg tttccagtga agcagatggt
     4321 gaatcagaaa tcgttttcaa tggcgtgttt gatgacgtaa tgggtgtaaa cgatgaggta
     4381 ggcgtaggtg gaactggcat ttttggccgg gttacttctt gtcctgatgt tatcattgac
     4441 cgtttaaacc caccttcacc attccctgta agaataacac tcgattttgg tactggctgc
     4501 gtaggtcgtg atggccattt tcgcaagggc aaaatagtaa cagtatatac cggaaggcta
     4561 atagttccgg gatctgttgc tacaaccgaa tttgttgatt tttactttga tagtgtaaga
     4621 gtacagggtg ttcataagat aactaatacc agttcgccag tggcaaatat tccgccagcc
     4681 aggcagttta aagctgaagt aatcaacggt aaactcaccc gtcccaatgg caactttacc
     4741 gaatggaaca gcgttaaagt aattacccaa atagagggtt tggctacccc aattccattg
     4801 gatgatattt ttaaaattga aggttctgca aggggtcgtg taaaaagagg taatctctta
     4861 actatatggg aatcaaacat tattgnnnnn nnnnnncact agtcaagcgt ttcaactgcc
     4921 gctggattgt ccgtggaaag ataagaatag taagggccaa tgccactact aatagcccgt
     4981 ggattgccgt tctggacttt ggtaacggta attgtgataa ccaggctaca attactatca
     5041 atggagtaac ccatcaaatt actttgccgt aattaaaaaa ttattatacc tttaaaccag
     5101 ttttcatagg gtacggatta aaaacgggcc agggtttcta cccaggccct aactttttta
     5161 agtaggtccc ctcaatttta tagggtaagc gataagaact tatactcttt tttactaaag
     5221 tgttcaagta cctgcggcag tgaatactgt aatttatccc atgctttttc actgtcatga
     5281 aatacaacga ttgatccgga accggtgttg tttaaaacat tatgcaaaca ggtttccttc
     5341 gaaattctca aatcgaaatc accgcttaat acatcccaca tcacaatctt atagacactt
     5401 aacttcttta cctgacggga acggatgcgg ccataggggg gtctgaagag tgcagattga
     5461 atgcttcttg cagctaacct tacatcatcc aggtaaactt catctgctgt tttccaacca
     5521 ttcaaatgat tttgtgtgtg atttcctgtt tgatgccctt cagcgataat acgatcgtat
     5581 aaccctggat tactttctac attcttccca atacaaaaaa atgtcgcttt ggcattatan
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaccccat accctgtgcg
     5761 ggtacatttt cttcaaccac cagggagttt tgataaaata tttaagcatt tgtacaaata
     5821 tagctcccct ttagaggttg gtggttttat ctttgcagcc tatgagtaaa aaagtaaggg
     5881 ttcggtttgc accaagtccc accggtggtt tgcacctggg cggtgtgaga acagttttat
     5941 acaactacct gtttgccaaa aaaaataagg gagatttnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     6061 tggtgtggcc tggaacctga tgagagtgta cagcatggtg gcagctatgg tccttaccgc
     6121 caaagtgaaa gaaaagaaat ctaccggcag tatgcagaaa aattggtggc cgaaggtcat
     6181 gcctattatg cattcgacac ccctgaggaa ctggaaaaaa tgcggcagga ttttaaaact
     6241 gcgcaaaacc cttctccgca atacgaccat accatccgca tgagaatgcg gaattcactc
     6301 acattatcta cagaaaaaac aaaagaattg ctggatgcag gaaccaggca ggtaatacgg
     6361 attaagatgc cggaaaaaga aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnaac ggtggatgat aaagtactcc tgaaagctga tggtatgcca
     6481 acttaccacc tggcagtggt ggtagatgac catttaatgc agatcagcca tgctttccga
     6541 ggtgaggaat ggttgccatc agcccctgta catatcctgc tttggaaata tttatttgga
     6601 ttggaaaaca tgccgcaatg ggcacatttc ccgttgatac tcggccccaa cggcaaactc
     6661 agtaaacgag acggagctaa atatggtttc ccggtatttg ccatgaactg gaccgatccg
     6721 aaaaccggcg agttgaccga gggtttcaaa gaaaaaggat ttttacccga agctttcatc
     6781 aacctgctgg cactgctagg gtggaacgac gggactgagc nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agtttgatta cgaaaaagcc
     6901 aaatggttca accatgaatg gatcaaaaga atgaaggttg aagatttaaa cccgaaggtt
     6961 agtgaacttc ttgttcaaag tggtattact attactaaca aagacctgct agacaaaata
     7021 attacactgg taaaagacag gtgtacgctg ctaactgatt ttatacaaca atccaccttc
     7081 ttctttaaag caccggaaga agtagatgtg gatgcagtaa aaccaaaatg gagtgaggct
     7141 aagaaacttt tctttgctga gcttatacgc aattatgagt tggcccctga ttggaatgcc
     7201 catactttag aaaccaattt taaagaaatt gcatccgtaa atgacctgaa accaggtgaa
     7261 ctgatgctgc ccttccgtat catgctggtt ggaggaaagt ttggcccggg agtgtttgaa
     7321 attgcggaat tgctgggtaa agaagaaaca ataaaaagaa tgcaatttgc aataagcaag
     7381 ttttaacctt gtagcctgat taattccata acctgaagga aatcattgca accccgtttt
     7441 tcgatcattt cccggtcagc catatccagt tgaaataaac cggtagtatt ttctgccaca
     7501 tgtttgctgg ttcggattcc gggtataatt accgacacct cactataact caaaacatag
     7561 cttaatgcca gctgtgtttt ggtacaatta tacttttcac acaattccca aacgggtgcc
     7621 agtgcatcgc tgcatacact caccacttca ggagtaagcc tgtttttacg atgatcattt
     7681 tcaggaaact cagcctcctt atcaaatttt ccggtcagta gcccaaactg cagtggcatc
     7741 cgggcaataa taccgtatcc tttttctacg gcctttttta agagcggcag ggcattttgg
     7801 ttcaatagat tcaataccag ctgaaagcca ttgcccagtt cttgctgcaa taaatattcg
     7861 gcttctgtca atggataaaa agtattcagg gaaagtcccc aatacctgat tttcccctcc
     7921 tgttgcaact gctgcatcgc ttcaatacaa tcgccttcct tcaaatgact ctgtctcgct
     7981 gtgtgaagct gataatagtc tattacttct cgtttcagtc gcttcagcga agcttcgcag
     8041 gcacttttta tatgctgacg actgtaatca acagtaaact cattattccg ggaaacatta
     8101 cctgctttac tggcaatgat cacatctttt atgccaccaa ttgttttacc tagtaattct
     8161 tctgaatgac ccaggccgta tatatcagca gtatcgaaaa aattaattcc tgcgtccaga
     8221 gaggcataaa ttgccttttc agaaacttca ttatctgcat ttccccagcc aattgctgta
     8281 gtaccaacca tggccccgcc accgatagcc catgctccaa agcctacctc actcaccaac
     8341 aattcagtag cgccaaactt cctgtattgc ataacctaat atacgctgct gaagctgaaa
     8401 gaacctaatt ttgcaataca atattttgcg atgaaaagac ctatcagagt tttggtggcc
     8461 aaagttggtc tcgatggcca tgacaggggt gccaaagtaa ttgctacagc actgcgggat
     8521 gccggtatgg aagtaattta taccggtctc aggcaaaccc ctgaaatggt agtaaatgcc
     8581 gctttgcagg aagatgtgga tgccataggg atcagcattc tttccggcgc ccataatacc
     8641 gtttttccta aagtgataaa cctgatgaag gaaaaaggta tggacgatgt actcctgacc
     8701 ggcggaggca ttataccgga cgaagacatt gaagaattaa ataaaattgg tgtagggaaa
     8761 ctgtttcatc ctggtaccaa tactcatgag atagccgatt atatcagtaa ctgggtaaaa
     8821 gaacaccgca atttttaaaa ctaaaattac atgtatcaaa cactgcttgc cagcctggaa
     8881 aatggcatat atactattac catcaaccgc cctgataaat taaatgcact gaacaaagat
     8941 gtatttactg acctgaacac tgctttagac gaaattgaga gcaacccgga aataaaatcc
     9001 gttatcataa ccgggtctgg accaaaggca tttgtggccg gtgctgatat ttcggagttt
     9061 aattccttaa ataaagaaca ggcaaaagct ctggccaaaa gaggacagga catttttgcc
     9121 cggattgaaa actcttctaa acctattgta gcggcagtaa atggttttgc attaggcgga
     9181 ggttgtgaac tggcgatgag ctgccatttt cgtgtagcca gtgacaatgc aaagtttggc
     9241 caaccggaag taaacctggg tttgattccc ggctatggcg gtacacagcg attagttcag
     9301 ttgatcggaa aaggcaaagc aatggagcta ttaatgtcag ctcatatgat tgatgctaaa
     9361 gaagcaaaag agttgggcct cgtcaactat gttaccacta ctgatacact acttgaacat
     9421 acccgaaaaa ttctggatgt tattaattca aaggcaccgc ttgctgtagc cggatgtatt
     9481 aaagctgcca atgccgtatt taatgaaagc atagatggct ttgcgctgga gattgaagaa
     9541 ttcggtaaca ctttcgatac agaagacatg aaggaaggtg tgactgcttt ccttgaaaaa
     9601 agaaaagctg atttcaaagg caaataaacc ggctactgtt ccaccaagct ctcatcgatc
     9661 aggtaggcgg caataccggt gatattgtca tttccctgcg catcttccag gtaaacacgg
     9721 aatgaggtaa tggtggtagc aggaaaagtg agaattcgct ttctgcctac actggtaccc
     9781 tgcacttcac caatgggttt atcattggaa tataacacta tgctaaaccg gcgtatactt
     9841 tgtcctaagt gtattgcctc ccgaagaaca atacaatttg tttttaccgg ttgcgacagt
     9901 tgcacaatga agttctgcag attaatacca tagtaagcag atgtggtatc aaatcctctc
     9961 agtaaaagag gtttattgta aagatctttt tggctaaact cataatagct atctgcatcc
    10021 tttaataagt tggtagcaaa attttcatcc cgcatttttc tgaattgcat taattcagca
    10081 gagtctgctt catgaattag ccctctttta tctggcggta tatttaataa caggttagcg
    10141 ccacgaccaa ccgatttcag gtatatatca aagagcttgc cggctgattt tactttgtta
    10201 tcctccgctt ccctgtaaaa ccagcccggc cggatgctca catcacattc agcaggaatc
    10261 cagtgtttgc cataataatt cccatgatta agggtgtcat tcgcaggagc accaatgcct
    10321 cgtttataac caacagtatc caaaaaattc cagttagggt cacccacaaa tccattttca
    10381 ttgcccaccc agcgaatatc agggccaata tcgctgaaca caactgtttt aggtgaaagt
    10441 tgtcggacag tattctcata tcttctccag tcatattcct gtcttttacc gttcggacct
    10501 tcgccatttg ctccgtccca ccatagttcc catacaggtc catagttttg aaatatttct
    10561 ctcatcatgc ccacaaatac atcattatat ttatctgttc cgtaatcagg atggttcctg
    10621 tcccacggag aaatataaac gccaaacttc aacccaaatt ccctgcaagc ctgtgaaagc
    10681 tctttcaata catcaccctt tccatctttc catttgcttt ctctgactgt atgctttgaa
    10741 tatttacttg gccaaagaca aaatccatca tggtgttttg ccgtaatgat aattcccttt
    10801 gcacccgccg ccctcgctat acgacaccat tgcctgcaat ctaattcggt ggggttaaaa
    10861 atatcttccg gctcatttcc ttcgccccat tctttgccgg tgaatgtatt ggggccaaaa
    10921 tgcatgaata aataaaattc attttcatgc caggctaatt gtgcctttgt aggtaaaggt
    10981 gaaatttcag ttttctgagc agatacaaaa gttgaaacaa aaagaataaa aaacagaaaa
    11041 aaaattctca tttcttcatt ttaaattcgt aaagaatagc gtcagcaatc gggtgcgtcg
    11101 ctttataagt atagccgagc agtttagcca tggtctgagc aaattgcttc tggtaaagca
    11161 gcactgaagt attaatctct cccctttcag gagtatcagg ccctaacact gcaaaccaga
    11221 tctcattggc cccttctatg ctgcttccat gacttgtcca ttcttccttc tttatatctc
    11281 ccctgccatg atcggttgtt ataaataagg ttgttttatc cttgtactgc gggtcctttt
    11341 gtacaaattc ccatatctct tttatccatg catcaacctg cctggcggca tcaaggtaag
    11401 accggtaacg gcctgcgtgg gcccactcat ctgtttctcc ataaccgata tagagtaccc
    11461 ttggttttct tgttttcaac cactccatgg ctgcataatg cgtaaatata tccaggcatt
    11521 catcctccag ccagggcttg taagaatcgc taagcatttg gtttatcagc ttttcattag
    11581 ctgttggtga tttatcaccg gtaatatcaa aaccgcagac cactggtatc ttgcttcttt
    11641 cctcatttaa aattctgtca aatgcgttcc aggcaccaaa ggcggctact ttgcccttca
    11701 gggggggctg attattcaaa aactccaata ctgatacatg tggattggga ggatagttat
    11761 tgctgttgat ggcagtatca gcatatcctg taaatatttc actataacca gggtagctga
    11821 accaataggg attggcatta tctactttgt tacccaatct cctgtttcca aatacctgtc
    11881 ctttcgatac gatagtactc caaagaaatg gcattagttt ttttcttctc tccctctcat
    11941 cagctgacca ataggatgaa tagatataag tactgtctcc ctcgttatac ttagatttat
    12001 tggcgattga cgtatccatc ccctggaaaa cctcctgcca ccgcaatcca tctgtagtta
    12061 tgataatgat attcctggtt tgttgctgtg agctgccacc gatagataaa agaagcaaaa
    12121 agagaaagca aactgatttc attgggggaa attagcacac ttttcttatc aaaaatcagg
    12181 tgcccgattt attgattggc ctacctttgc gggacattta aaaaccctct ctactatgga
    12241 atacaggatt gaaaaagata caatgggcga agtaaaagtg ccggctaatg cctactatgg
    12301 cgcacaaacc caaagaagta ttgacaattt taagatcgcc caggatatca acaggatgcc
    12361 aaaagaaatt atacaggcat ttccctacct gaaaaaagca gccgccataa caaatcttga
    12421 agccggagtg cttcccaaaa acaaggcaac attaattgga aaagtatgtg atgaaatact
    12481 caaaggcaaa ctggatgact acttcccgct ggtagtttgg caaaccggaa gtggtacaca
    12541 aagcaacatg aacgtaaatg aagttatagc ttatcgtggc catgtactga aaggtggtaa
    12601 ccttattgat aaagaaaaat tccttcaccc gaatgatgat gttaacaaat ctcagtcatc
    12661 caacgatacc tttcctactg caatgcatat agcagcctat aaaatattgg tagaaacaac
    12721 gatacccggt ataaaaaaac tgcgagacac attggccaaa aaaagcaagc agtacatgca
    12781 tgtggtaaaa ataggacgga cccactttat ggatgccact cctttgacgc tgggccagga
    12841 gttcagcggt tatgtatcac aattagatca tggattaaaa gccataaaaa attcactctc
    12901 gcaccttagt gaactggccc tgggtggtac cgctgttggt accggcatca atacaccacc
    12961 caactatgct gaaaatgtag cgaaacatat tgcgaagcta acaggcttgc cattcgtaac
    13021 agctgaaaat aaatttgaag ctcttgcagc gcatgatgcc attgtggaag cacatggcgc
    13081 tttaaaaaca gtagctgtaa gcctgatgaa aattgccaat gatatccgca tgctttcttc
    13141 tggtcccagg agcggtatag gtgaaatatt tattccggat aatgaaccgg gatcatccat
    13201 tatgccgggc aaagtaaatc ctacacagtg tgaagcattg accatgattg cagcacaggt
    13261 aatgggtaat gatgttgcta tcaacattgg cggcgctaca ggtcattttg aattgaatgt
    13321 tttcaaacca gtaatgatct ataattttct gcacagtgcc aggcttattg gtgacggatg
    13381 tgtttccttc aacgacaaat gtgctgttgg catcaaaccc attgaagcaa atatcaaaaa
    13441 acatgttgat aacagcctga tgcttgtaac atcattgaat acaaagattg gatactataa
    13501 agcagcagaa attgcacaaa aagcccataa agaaggaact acactaaagg aaatggcagt
    13561 aagacttggc tatgttacac ctgaacagtt tgaccaatgg gttgtcccag ccaatatggt
    13621 aggtaagctg gagaaataat tatcgtaaat ctcctatcgt aaaaagagaa gaattcatca
    13681 ttcttctctt tttttgttca ttttgttttg gcgaaacaaa aattaactta ctaccttaac
    13741 agtagtataa atccaatact cataaaaacc agtaaatccc tgactatgaa aaatgcagcc
    13801 catccatcac tctttttcct tgcaaaccca ctaaaagcag tgcaagcaga acccgctgct
    13861 gctgcagaag ataattacca gacaccgtac ttacgatcaa tactttccgc taagtcagca
    13921 ccttccaggg tctattcact aagaagcata cgggaaaaat ttgtaagcac agtgagacct
    13981 tttatcagca cactgccaaa agatgtcgaa ttatccgaaa aaaactggtt caataactac
    14041 gagtaaccgt aacccttgag cataaccaca aaaacaaatg tcccggcagt tgtcgggact
    14101 ttgcttttaa gaaagaatat tctactattc attttatcgt tcatttccga ttaaaataac
    14161 taatggttta caccagccag cagtaataag aaactttcat gtaaaaacct cttgggattt
    14221 tttatacgta tttaagtaga aagtcctatt gttcctgatt acagattaat ctatttttaa
    14281 tatccggagt cagacgctgt gaaaagaaaa tccgatccat gaaaagaggt gtacccaata
    14341 ctttctatcg aatattttta accgtaacaa ttataatact aatacttacg gttagttctg
    14401 gatatattca tgcacaaact gtcgacttcg gtaaaagcta tatcaatgtt acaaagggtt
    14461 tgaacggcgg cactcttgaa acaggtgaca cacttgaaat aagagcatct tttgttgtcc
    14521 gctcaggcac ctatgatagc tgccgctata gagataatgt acctacaggc acgacattta
    14581 ttcccggaac gatacgggtg ttgactaacg agggcaagat atacaaacag ttaactgatg
    14641 gctatggtga tgaccccggc tggattacag gaacacaagt aactatacat ctaggtttta
    14701 atccatcagc agcacctgca acagccttca gaagaggaag agtagctaac acacacaaac
    14761 cttcctttta cagtagcacc tgcattatga ttgcatcttt cagagtggtt gtcactgctg
    14821 tgacaggttc aaatatcagt accggcgggg gaacaattac ttacaaaagc ggatccaatc
    14881 ctattcaaac atatactttc ccttcaaata ctgtgagagt atatccaaac tatggcattt
    14941 gttccaatac gattggcacc aatgcaatag gtacagagtt taacggcaca tttgggagcg
    15001 gtcaaccacg gaacagggga acctctgcca atgtaccaac aggttacaca tacaatatct
    15061 tcaacaccaa ttctccacag gattattatt atggaatcgc taataatact tctacccgtg
    15121 ttaattatac aacattaaat acatgggcta aaccagataa ttcgtctcca acacacaggg
    15181 tatttaatgt ctgggacatt attggtgacc atactggtgc caccgaccaa tattcaggaa
    15241 atcctcctgc tgatacagtt gccaatccca atgcgggata tatgctggtg atcaatgcgg
    15301 catacagaat tgattccgct tttcagcaaa caattactgg gctttgtcct aataccttct
    15361 atgaaatttc ctgctggatg cgtaatattt gctccaaatg tggttgtgat tcaaatggaa
    15421 ggggtgccac aagctctgct ggcccacctt actatattcc aacagcaacc ggagattcat
    15481 ccggagttcg cccaaatatt actttcgaaa ttgatggtgt tgactactat actaccggcg
    15541 acctgctcta tacaggacaa tgggtaaaga agggttttac ttttcttact ggtcctgctc
    15601 agacaagctt tactttaaaa tatttcaaca acgccccggg aggtggtggt aatgactggg
    15661 cccttgatga tattacagtg gctacctgct ctcccgatat gaagtatggg cctatgaata
    15721 atccaatagt ttgtgatagt aatgtgctgg tgcttagtga tacgatccgg tcctatttta
    15781 ataactacac cgagtataaa tggcagcgca gtactgacgg tggtgccacc tggaatgata
    15841 tacccggagc aacaggatct ggtgtacctg tatggaatgg tactgcctgg gaatatgttg
    15901 cttactatac catacccatt tcccaaacac aaatgtccaa caacggtgat atgtacaggc
    15961 tggtagttgc cactacagtg gcaaatctat ctaacgacaa ttgtagtttt agtgatgcac
    16021 tcactattac tgtaagtgtt attgaatgtg gcataccctt acatgcacga ctgctttctt
    16081 ttagtggaat tctaaattct cagcaacgca cagatttgca atggatcacc accggagaaa
    16141 atgaaccagt actatttgat attgaaaaaa gtttcaacgg aagtaattat acagttatcg
    16201 gaaccataaa cggtcacaac gattattcct ctgaatacag ctattattcg tttactgatt
    16261 ctgctcccgt aagcggtaag gtttattaca ggattcggat gagaaataat actcagtccg
    16321 tttattcaag aactatccag gtatctaata ttgcagatca ttgttctttt gtttctgtca
    16381 taaatccgtt taagcataaa cttgactttg aacttagtgc ggctactcaa gaaagagcag
    16441 aaatattgct gattgatcag tcaggcagaa ttgtaaagca aaaagtaatt ttactttcaa
    16501 acgggattaa taaccttagt attgaggata ccgagaaact acaacccggc atatattact
    16561 taagagttgc agcagggggc actgttttgc aaaagaaagt tttgaaacag taaggcggtc
    16621 aaagggaatt acgaaacctg caccttatca cttacatcgg tatttgtttc aacggttgaa
    16681 tgagcatcat ccgccatatg cttcttgaaa aatctcttct gaaagatcaa caaggtgata
    16741 acaccaatag aaatagaagc atctgctata ttgaaaatgg ggctaaagaa ttcaaaatcc
    16801 tgccctccta caaaaggcat ccaggatgga aaatttgact taattatagg aaagtaaagc
    16861 atgtccacca cacttccatg caagaatgat gaatagccac tggaagaaaa ctcagcaatg
    16921 cctgaataag ttacgtattc attaatagcc gcatcataat gaaggccttt atcaaagatc
    16981 atcccataaa acatactgtc aataagatta cccaacgctc ctgcgtaaat cagccggcac
    17041 atacaacgaa tccacgactg tattgttgtt ttacaatttt tccaaggtac catgttccaa
    17101 atattactgc agcaagacgg aataaggtga gaatcatttt tcctgtttcg ttaccaaact
    17161 tccagcccca tgccataccc gggttttcaa taaaatgaat acgaaaccag tccataccaa
    17221 atacccgcaa cacttcccca gttggatgcg atgttttgat ccatatcttc aatgcctggt
    17281 cgacaatgat gattatgctg atcaaaagta ttacgtggcg tagcttaagc gtcttcataa
    17341 tagggacaaa tataagaaga ttgaggtgag cagataaagc gaaccggtga aagaaatcat
    17401 aatannnnnn nnnnnnnnnn nnnnnnntat tcttcaaaat aaacccgctt accccgttga
    17461 gaaatacccg tcataatttc atatggaata gtttctgccc attgggctac cgattgcacc
    17521 ggcaaggatt caccaaaaat aagaacatca tcaccctctt ttactccggg cacatgtgtc
    17581 acatctgcca tgaacatgtc catacagacc gttccgatta ccggcactaa ttttcccctc
    17641 acaagtactt ttccaacccc attacccaaa ctccttggaa acccatcagc gtatcccatt
    17701 cggatagttg cgatcagtga ctctttctca atctttccct tcctgttata gctgacagaa
    17761 tcaccttcct gtaaacgctt gagctgcgct atagtagatt ttaatgtagc caccgtttgt
    17821 aaattcagta aacctgaagc ggcgctgtca acaccatata gcccgatgcc aagccttacc
    17881 atgtccagtt gaagacccgg atgtcttact gcggctgctg agttggcgat atgcttaaga
    17941 aatgagtagc ctaatttttc ttccagttct gacattgcat tattaaaaac cgagtactgg
    18001 gttaatgtaa aatcatcatt cgctttttcc tcacttgctg caaagtgaga aaagacagtt
    18061 tcaaccctga aagatggaca caacaacaaa taatcagcca gttttccgat atctccaggc
    18121 acaaagccca acctgttcat acctgtctct atttccagat gaacgggata ttgttgaagc
    18181 ccctgctgtt gcaggaaata atcaaacgac tgcaacaatt caaaagaata tatctctggc
    18241 tgaagatcgt attccacaat agcatcaaac gcattctctt ccgggttcat caccattatt
    18301 ggtaaggtaa ttccagcttt tcttaactca acaccttcat ctgcgtatgc aacaccgagg
    18361 tagtctactt tatgatattg taaaatacct gnnnnnnnnn nnnnnnnnnn nnnnnnnnca
    18421 tggccattat cttagtagtg ggtttaagcc tgcgctggta ttccttcagg ttatgaacta
    18481 tggcagaaag atttatttct aatactgttt gatgaacttt ctgttccagc aactgaacaa
    18541 ttttttcaaa agcaaatatc ctggccccct ttaccaatat cacttcttcc ttaaattgag
    18601 aagaccgaaa ctggtggata aattcatctg ttgtccggaa gaggataata tcaatatcgc
    18661 tatatccttg cggaaaatga taattaattt gtttcgtaac cctttcacct atcccaatca
    18721 ggcggttcac cttatgcttt accaggcttt ccattacttg ccggtagagt aattcatcag
    18781 caattgaact ctgtaaaaaa tccgaaagta taactgtttt tttatgggag cctgcctgct
    18841 ggccaaggaa attcagtgct atttccaatg aactgatatc agcactgtag ctgtcattaa
    18901 ttatcgaaca atgatttatg cccttcttga gttccagccg catattaact ggtgtcagta
    18961 aaagcattct ttcctggaca acttcctgcc tgtaccctaa atgaagcatt acctgccagc
    19021 aggttattgc attctgtacc gatgcgtcgt cagtaaaggg tatttgtagt tgatgcccct
    19081 ggccggctgt tattattgta taatcatttt gtcttttgat ttcttttacc cgcaattgtt
    19141 caggtaaaac agcatacttg aacaactttt ttttctctgt ctccttttcc tgcaatgaag
    19201 aaaacccttc actatgtgct tcaccaatat tcgttaatac cccgatcgtg ggctgaataa
    19261 ttttttgcaa tttttccatt tctcccggac gggaaattcc cgcttcaaaa atggcaagag
    19321 tatgctgctt attcatttgc catacactca aaggcacacc tatctgtgag ttataacttt
    19381 tggggctgcg cacaattgta taatcctgat gtagcaattg ataaagccat tcctttacta
    19441 ttgttttacc gttgcttccg gtaatgccaa taaccgggat agaaaattgc cggcgatgat
    19501 gagctgccag caactgtaag gcctccaacg aatcattaac ccttaagaaa ttagcctcag
    19561 ggtattcttt aatttccatt tgccggtcta caacaaaatt cattactccc cttttataca
    19621 aatcagcgat aaactggtga ccatctcttc ggggtccctt taatgcaaaa aaaagtgatg
    19681 atgcgggagc aaatactttt cgactgtcga gtaataaatt ctcaattacc gaatcatgga
    19741 taattacggc atctgccttt ataatttggg caatatgatg tattgtataa gtcacaaaat
    19801 gaattatccc ggtgactggc tttcatcagt cacatcttca ggctcacctt ttttctgcat
    19861 aaagatggaa tagaaaattg agccggaaat acaaaccatg atcactccga gtgaaatgat
    19921 tacctggatc tgcttgctta cccactcgct gatccagtgt tcacccagca tcttgcaacc
    19981 aataaatacc aacacaattg caataccctg ctgcaggtaa tcaaacttgc tgactgctcc
    20041 ccggagcagg aaaaagagtg aacgcaagcc aagtacggca aaaatgttgg aagtataaat
    20101 caccagttta tctcttgata tacccatgac cgccggtatg gaatccaacg caaaaacaag
    20161 atcaattgct gccaacatga tcnnnnnnnn nnacaacaaa cagcgtagtg taagctggtt
    20221 ttccatttac tatcacccta tatttcccgt tgccatcatg cggtaccagg ggcaggaatt
    20281 tcttcatgaa ccggtatatc ttgctttcct ggggattaaa ttcttcatca tctgatgccg
    20341 tgaacatttt gtagccggta aacacaagga acacaccaaa cacatataag atccaatgga
    20401 atttttcaac cagggccact cctacagtaa tgaatatcac cctgaagatg atcgccatca
    20461 ggatcccgat taagagtaca cggccataat atttttcctt aacagaaaaa gctgaaaaga
    20521 tgagtataaa aacaaagata ttgtcaatac taaggctcca ttccatcagg taaccactca
    20581 ggtattccaa tgccagcctt tcaccttctt caacccacat gaaaacaaaa aaagccagcg
    20641 ccaatccgac ccaaaaaaag gtttgataca atgcctgcct gatagtgatc ttctggtctt
    20701 ttttgcttaa aagtcccaaa tcaaaaacca atgccagtac cagtacaata ccgaatacga
    20761 gatactcaac ctgttctttt gtcatgtata aatattatat gatgtgcaaa gataagatag
    20821 tggaaacctg gttcagattc tgagatcctg ccccgtaatc tgccttaaaa accgccagca
    20881 tactttcttt ttcttatgcc ctggtataac cagccaaata aaatgaccaa cgctaccggc
    20941 aaggcaatat tgatgaactg ccaggtagtt ttttgttcct ttactttttg tccgtccagc
    21001 aaacgcagca ctatgtcttt atttcttgtt tcaataatac caggtttatt caccaggtat
    21061 tccaggcagt tcagcaaaaa ctcacggttg gcaaactggt actcaaactg ggagccaact
    21121 gtatagaaat tgatccccat aggtaatggc ccttcctttg gagatacatc gttcagcacc
    21181 atatcaccat cagctactac aatgaccttt cctatgccat taccctctgc caaaaaactg
    21241 ctgcctgtag ctgtaaggct gtctagttgg gaacgggaag ccctgttacg gtataaagag
    21301 gtgaactttc cctccagtaa catggccact gggatatcac ttttattaaa tttgatatct
    21361 tttggaacat ttttattctc attaagactg atcaaagctg gagtactgat gatcctggag
    21421 tcagcagagc tggaaagcag cactgtttta tttactcctg gcgtagcaat ggtatcaacc
    21481 gaattcacaa atctcccgga gacaagacca atatttttat tgattgtatg attacctttt
    21541 gactcaaata aagggtagta attccatttt aaaaattcga attgcggatt ctgcaaatca
    21601 ccacctacta caaatggcat ataatcacac tgcaggtcca ttacaagatc tgtattaata
    21661 cgtacgccat aacggaacaa ttggtcagta agattaaggt tacggtcaaa agcaacagtt
    21721 tctttaacag cactgagact atcctgctct gctatcaggt tgtctatgaa catcaggagc
    21781 tttcctcctc tcatcaggta ttgatcaatt ttaagcttct cctcctccgt aaactgaagg
    21841 gttggcttaa cgatcataaa tacatcaatc ctggttttat cattaatgtc tgtagatatt
    21901 ttactgtgct tatttaggtc aaaaatgcca agctggtaat catttttcag tgcatcctga
    21961 ataccataag tatttgcatc aaccggttca ccatttccaa ttgaataagc cacatgtggt
    22021 ttctcagtag ttatcaatcc atccagtgcc tttgcaaact ggtattccat taatgcttcg
    22081 gcactgttca tttcaacagg agtaatcatt ctctttccgc cggaatacag attaacaaga
    22141 gcctgtttgc ctttataacg cattaaagca acaggataaa ctctcttatt ttcctgtcct
    22201 tcggcaacct gaacagtaag gttaatggca ttagcgccta atcctattaa ggtatcctca
    22261 taggttttac ctggagtatc tgctatttct tcctggggtg aaataaacct gtaatgaatt
    22321 tttgaaccat tcctgtcctt cattaattgt aaaaattcat tggtgctatt ggccagtttt
    22381 ttaaacccgg cggggaactg gccttttaaa aacacatcaa tctgtacatt atcctccagg
    22441 ttcttcagca agtctttagt tgccttgcta agtgtatatc ttttttcttt tgtaagatca
    22501 aaacgggcat ggaaaactga agctgcatag ttgataataa aaagtagcag cagcaaccag
    22561 aaccaccagg cttttgaagc gaatatttta ttaattattt tcataggtta tcttttcagc
    22621 aggtttcggt tggtgatggt aagaaacaaa aagatcacac tcagaaaata cattatatcc
    22681 ctcgtatcaa tcaaaccccg gctgatacta cggtaatgaa aatctattcc cagcatttct
    22741 acataataat cagggccgtt ggccagtgcc ggcaacttgc tgatggcgct gaacccatag
    22801 tatagcaaag cacaacctat caaactaatg ataaaagcta ccactgcatt gttcgtaaaa
    22861 ctgctgctgc aaatacttat tgaagtaaat acagctgcca gaaaaaataa accgatataa
    22921 gaaccgatag tagcgcctat atccaatccc tggttggaag aaagttgctg tatggtaaaa
    22981 atgtaaacaa cagtaggtag cagggctatg aataccacta tcaaggatcc caggtattta
    23041 cctcccacca cctgccagcg gctgaggggc ctggtttgaa gaatttcaaa ggttcctgtt
    23101 ttaaattctt cagcaaagct gcgcatggta atagccggaa tcagcaacaa aagtatccag
    23161 ggagctaact ggaaaaatcg gtcaagtgtg gcataaccaa agtcgaggat actatcttca
    23221 aacacaaaaa gtactagtcc gttgaccagc agaaatacaa taatggcaat atagccggta
    23281 aggctgctga aaaattgccg gagttccttt ttannnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agatatgcaa ggcagtttaa ctttaatact
    23401 gatagctacc gttacgaaat gaaaagaggc tgacaactaa aacttgtcaa cctcgtacct
    23461 tttacttttt aacctgtgtc taaactgcaa aactctcgcc acatccgcag gtacggctgg
    23521 cattaggatt actgaaatag aaaccttttc cgtttaaccc gtcggagaaa tccaaagtag
    23581 tattgaccag gtataaaaaa ctcttcaggt cggttactac tttcactcca ttatcttcaa
    23641 atacctggtc catgggtttt tgttcattgt caaagtccag tttgtagcta agaccagaac
    23701 aaccaccgcc taccacactt acccgaagga agtaggatgg gtcatcagca accccagcat
    23761 cggccagcaa ttgctttacc ttggcctttg ccttatcact tacgtaaatc gcattatcta
    23821 acgatacact cataattaat gaactacgct ttcttcaaat ttcagttcag gtaaaccatt
    23881 ctttttacga taatcattta cc
//
LOCUS       DAORLS010000014        23641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1192_length_23641_cov_2.054778, whole genome shotgun sequence.
ACCESSION   DAORLS010000014 DAORLS010000000
VERSION     DAORLS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 23641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23641
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1192_length_23641_cov_2.054778"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    212..412
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <413..1649
                     /locus_tag="PK977_02395"
     CDS             <413..1649
                     /locus_tag="PK977_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF16982.1"
                     /translation="CVPGYKSIGIAAPIIVLLLRLIQGLALGGEYGGAATYVAEHSPA
                     EKRGFYTSWIQTTATLGLFVALGIIMLVKLNMPDTAFNAEWGGWRYPFWVSVVLVGIS
                     IYIRMKMSESPMFSKLKAEGKTSLNPITESFRHKANFKMVLLALFGAVMGQGVVWYTG
                     QFYAQNFLETKCNLEFEQSRTIMLWAIAFATPFFLFWGWLSDKIGRKWIMMAGMLLAI
                     LTYRPIFSTFLSDTDSGKWKNGQTTFIKEEIEKKVSDGDTILKITSHFKTNNGIKYSN
                     VLVDTVKAGTSTKKQVIKANLNSADLNKHLPPALFWKFIGLVFILILYVTMVYGPIAS
                     FLVEMFPTKIRYTSMSLPYHIGNGVFGGLVPFIGLLLTTTYPADPLVGLWYPIGVALL
                     CLIIGSLYLENKIDKNVMD"
     gene            1651..1896
                     /locus_tag="PK977_02400"
     CDS             1651..1896
                     /locus_tag="PK977_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16983.1"
                     /translation="MSALKKYFGLLLMLIAPLIIYQLVHGAISHIDPAGKKDINSPVV
                     WIIIITVFTPIAIGLVIFGWYAFRGEYDHLPHKSKEL"
     assembly_gap    1957..2071
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2072..2255
                     /locus_tag="PK977_02405"
     CDS             <2072..2255
                     /locus_tag="PK977_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16984.1"
                     /translation="VDEEKNNTKEDSVKNLKTYCSSGNFLVLLLSAQSISHFNTTERL
                     AVSPYLGNHTPPPNYC"
     gene            2345..>3147
                     /locus_tag="PK977_02410"
     CDS             2345..>3147
                     /locus_tag="PK977_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF16985.1"
                     /translation="MSAQLNPVTRILNLVRLERKEIGAIYFYAILGGLIQLSLPVGIQ
                     TIIGFILGATMSTSLKILIILVVGGVLAGGLLQINQMKIIEKIQQRIFVRFSFQIAER
                     IPKLDLKQADSYYLPELVNRFFDITALQKSLSKLLLDVPGASIQILFGLILLSFYHPL
                     FILLSVLLVIVLSVILYYTGNKGLETSLLESRHKYGVAAWLEEMARVIKSLKFSKGSH
                     FHMQKADERVSAYLQYRTSHFSILLFQYKMLVLFKTLITAAMLVIGSFLL"
     assembly_gap    3148..3157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3158..3987
                     /locus_tag="PK977_02415"
     CDS             <3158..3987
                     /locus_tag="PK977_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF16986.1"
                     /translation="QQLNIGQFIAAEIVIITVINSVEKLINNLDSVYDVLTSVEKIGK
                     ITDKPIEHSGSLPMKEGKSGYKIEMSNISFGYNEDKRIIQNLSVKIEPGQKVCIMGAE
                     GSGKSTVLRLMTGAYTEYDGNILIDNIPIKNYDIASLRSYTGIFLSQQDIFQGSLEEN
                     ITMGNEEIDRTEVIALFEKVGLLGFLTSLKDGFDTELDPTGHRLPKNVVHKLLLVRAL
                     VNNPRLLLLEDPFSGLEETFKLQIQSLLLDRNNPATVVISTNDEQFAQQCDKIVRIN"
     gene            4026..5345
                     /locus_tag="PK977_02420"
     CDS             4026..5345
                     /locus_tag="PK977_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF16987.1"
                     /translation="MKEKELNAYSQIYRHNKTSKVKYWFYGILGFILLFLFLPWTQNI
                     RARGSVTTLKMEQRPQEMNTIIPGKVVKWYVKEGDYVQAGDTIIQLSEIKDEYLDPNL
                     INRTQEQLSAKQMTIDYYKNKVDVTQTQIGALSSGRELKLNQLENKLAQLRLKVQSDS
                     MEAIAANNDFNIATQQYNRQKAMYDSGLVSLTQLEQRNQSYQNALAKKISAENKLANS
                     RQDLTITKIEMNSVQQEYLEKIAKAQGDQFQSMSQIASGQGDIAKLENQYSNYSIRNG
                     MYYITAPQSGQVVKAKKAGIGEVVKDGEMIVEIVPDKIQYAVELFIRPLDLPLVAPGQ
                     KVQFLFDGFPAIVFSGWPNASYGTFRGKVVAVESSVSSNGKFRILVAEDADYKPWPKQ
                     LRVGTGAQGMALLKDVPVWYELWRNINGFPPDYYKPATTTNKNETKK"
     gene            5329..>6691
                     /locus_tag="PK977_02425"
     CDS             5329..>6691
                     /locus_tag="PK977_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF16988.1"
                     /translation="MKLKNKQILIAGLFALCFSAKAQNNQEKTLSPSQLVEMVRKFHP
                     VAKQADINIDKAKADITIARAGFDPYFYNSSAQKTFDGTDYYYYNRPQVSIPTWFGVE
                     ISGGREYLSGSRTNNEETLGETSYFGITVPLAKNLLMDKRRAALKTAKIFREASVIEK
                     NNILNNLMLEALNMYWEWTGQYQLYNILTNAVKVNEQRLQLVRIAYRQGDRPAIDTTE
                     ALTQLQSFQLLQSQAWLSFQNSGLELSTYLWTTENLPFSLPEDVIPDEESRGLNTIKL
                     NVPELNQLLDAARRNHPELLQYNFKLDALDIEKRLKFQDLLPSVNFRYNQLGKGYDIL
                     KTATGPLFENNYQYGLSIGIPLRFSQGRGEYKKARLKISETKLQQNLKQLQIENKVRS
                     YYNELNTLRQQISLQEKAYQNFMILQRGEETRFQSGESSLFLINSRENKSLEALQKLT
                     ELKA"
     assembly_gap    6692..6727
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6843..7208
                     /locus_tag="PK977_02430"
     CDS             6843..7208
                     /locus_tag="PK977_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF16989.1"
                     /translation="MAKEHIYYSLYHKSSSILMILALLWLTVSIPYVFNAQKQLLSQE
                     KQQPVPVNSQQEEEAANPFASTTEEKTPGSGNTLSEFLHEIELLKHPSRNLTRYFMYI
                     RSSLYIAFHGELLVPPPNC"
     assembly_gap    7251..7341
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7386..8933
                     /locus_tag="PK977_02435"
                     /pseudo
     CDS             7386..8933
                     /locus_tag="PK977_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            8960..9592
                     /gene="can"
                     /locus_tag="PK977_02440"
     CDS             8960..9592
                     /gene="can"
                     /locus_tag="PK977_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HRF16990.1"
                     /translation="MKSYEKLLLENRAWASEKVSEDPDFFNRLAHLQTPEFLWIGCSD
                     SRVPANEITGTQPGEIFVHRNVANMVVHTDLNLLTVLDYAVNHLKVKHVIVCGHYGCG
                     GVKASMTKHNFGIINKWLRNIKDVYRFHREELEEIKDEEARADKLVELNVQEQVMNLA
                     KTSIVQKAWAHHQRPHLHGWVYGLKDGIIKPVFEMPAGTHIDDLYEFDDL"
     gene            complement(9597..>9975)
                     /locus_tag="PK977_02445"
     CDS             complement(9597..>9975)
                     /locus_tag="PK977_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HRF16991.1"
                     /translation="ESVLQKPMSEQKSSGHLHPGMFKKHYSPKTPLWLMNRNSDFTRL
                     RFQKNIAAICFTENFPSVPVKEKIMLSNSGDLEEAAQHLYSVLYELDKGNFDLIIAEK
                     MPDAGLGKAMNERLIKEAHKHYN"
     assembly_gap    9976..9990
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9991..10565)
                     /locus_tag="PK977_02450"
     CDS             complement(<9991..10565)
                     /locus_tag="PK977_02450"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRF16992.1"
                     /translation="MKGSSVQLAAEWLRKGEVVAIPTETVYGLAGNAYNVDAIQKIYD
                     VKNRPLSNPLIVHALNIQQVKSLVKEFYAEAEILAKSFWPGPLTMILPKAAIIPDIVT
                     AGQQTVAVRVPSHPLTLELLSLLEFPLAAPSANKFNYISPVTAEAVSEMLGNEVPYIL
                     DGGRCQKGIESTIVSFVEKQIKILRHGAITE"
     gene            complement(10583..12037)
                     /locus_tag="PK977_02455"
     CDS             complement(10583..12037)
                     /locus_tag="PK977_02455"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRF16993.1"
                     /translation="MQHSLNTPVAIAYGDGIGPEIMRSTLEILQAAGARIDFQEIEVG
                     EKQYLKGHTSGISPEAWQVLRSTKVFLKAPITTPQGTGYKSLNVTIRKSLGLFANVRP
                     CMSYHPFITTHHPEMDVVIIRENEEDLYAGIEHQQTDEVTQCLKLISKPGIEKIVRFA
                     FEYARVYGRKKVSCFTKDNIMKITDGLFHRMFDEIGNEYPEIKKEHYIVDIGAARLAD
                     TPEIFDVVVMPNLYGDILSDVAAQITGSVGLGGSANIGSACAMFEAIHGSAPDIAGQD
                     VANPSGILLGAVQMLVYIGQHDVAQKIHNAWLKTIEDGIHTRDIFREGKSKVQVGTRL
                     FTKAVIERLGEVPQQLPAVTYTMEGDKKITISMPVPKISKKELVGVDVFLHWNKDDRN
                     PEVLGNQGSKYKAGHFALNMITNRGVKVYPGGIPETFCTDHWRCRFVADAPGKYAEVL
                     ELCGQLSQEGFDIIKTENLYNFDGKAGYSLGQGQ"
     gene            12142..12816
                     /locus_tag="PK977_02460"
     CDS             12142..12816
                     /locus_tag="PK977_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF16994.1"
                     /translation="MELAVQIKMNEKLFLRNPELSELGRNIIKYSVVMIREKGFEDFT
                     FKKLAQRIGTTEASIYRYFENKHRLLIYIVAWYWSYLEYKVVFYTQNLANPENRLKKI
                     IELLAKPVTDDFSTDYIDEKLVYEVAVWEGSKAYLTKHVSKDNKDRLFKPYKDLCGRI
                     ANIIAEFNPRYKFPRSLSSTLLEMAHYQRFFMNNLPSLTDFGEDKSEERLYQFLYQLV
                     FDSLKK"
     gene            12828..13802
                     /locus_tag="PK977_02465"
     CDS             12828..13802
                     /locus_tag="PK977_02465"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HRF16995.1"
                     /translation="MDYNLILSNLTNPTLLFFLLGVIAALIKSDLEIPVQSSKFISLY
                     LLFSIGFKGGMELSHSGINKEVAISLLLAIFFAVTVPVYSYFILRRKLDVYNAGAIAA
                     TYGSVSAVTFVTACSFLADKNVEFGGHMVAAMALMESPAIIIGMILIRKFSSEKQQQP
                     FSHIIREAFSNGSVLMILGSLVIGIVADEKQAEGIKPFTSDIFKGFLAVFLLDMGITA
                     TNRIKAFNNGGLFLALFGILLPVFNACLAIFFAHSLGVHDGNAFLIAILASSASYIAV
                     PAALRLAVPQADPGYYIPMALAITFPFNIVVGIPLYYGLLSVLKFESI"
     gene            13799..14065
                     /locus_tag="PK977_02470"
     CDS             13799..14065
                     /locus_tag="PK977_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRF16996.1"
                     /translation="MKCPSCNETLVMTERQGVEIDYCPKCRGVWLDKGELDKIIERAA
                     GEQPGNTSKSKKQWDDDDDDDDNDFNRKGGRRKGGFLGDLFDFG"
     gene            14074..14982
                     /locus_tag="PK977_02475"
     CDS             14074..14982
                     /locus_tag="PK977_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRF16997.1"
                     /translation="MANTWLWIGINVFVLLMLELDLGVFHRKEHKVTFKEAISWSVVW
                     VTLALIFNGWIYYDQGKDKALEFLTGYLIEKSLSVDNIFVFVLLFSFFKVPEQYRHRV
                     LFWGVLGALLMRAGFIAAGAVLIEKFHWIIYVFGAFLVFTGYKMLNKKTEDMHPEDNP
                     LVRWFVKKGKVTSDYHGKNFFVRINGQKLATPLFLCLLSVEFTDLIFAVDSIPAIFAI
                     TKDPFIVYTSNVFAILGLRSLYFALEGIITRFPYLRYGLALILIFIGLKMLAVDIYKI
                     PVVASLAVIAVILLTSIFWKGKKQPG"
     gene            15042..15950
                     /gene="gldA"
                     /locus_tag="PK977_02480"
     CDS             15042..15950
                     /gene="gldA"
                     /locus_tag="PK977_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRF16998.1"
                     /translation="MSIEVNNLLKEYGEQKAVNNISFKINKGEIVGFLGPNGAGKSTT
                     MKIITGYLQQTGGEAFVCGINVAEQPLETKKKIGYLPELNALYYDMYVREYLGFVAEV
                     HAIKDPKSKIENVIDTVGLTVESKKKIGQLSKGYKQRVGLAAALIHDPEVLILDEPTS
                     GLDPNQIIEIREVIKKQGQNKTVLFSSHILQEVEAICDRVIIINKGQIVADDKLSNLQ
                     KSNKESHIVIVQFKEAIDKNRLTGMKQVSKIEEAEHNLFKLHTSDPEAVRKQILELAL
                     QQQLNILSLQSESRSLEDVFRSLTHN"
     gene            complement(15957..17915)
                     /locus_tag="PK977_02485"
     CDS             complement(15957..17915)
                     /locus_tag="PK977_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF16999.1"
                     /translation="MQAYSKHILLSLLAAVNTLAVFCQGSQCPSNIDFETGDLNNWEC
                     KTGRVESIAGVNTVTWESSGVNPLNHQIIPRATAGLDPYGNFSQASPNGSAYSVQLGN
                     NYFQNISAEGMFYTFSIPDTATLFSILFYYAVVFQDPQHNPDEQPRFRARVYNVTDNE
                     VINCANFDFTASASLPGFTVSTVDPTVIYKDWTPVTINLSGLAGKTIRLEFITSDCTF
                     RGHFGYAYIDVSGSCGGAVTGSEVCADADEVTLLAPYGFQTYSWYADQTFSTVLSSSQ
                     ALTLSPVPPAGTSFPVVVGPYPGFGCPDTVVAVLKTVTLPVANAGIDVTTCKNQFAQL
                     GTNPLEGYGYSWTPPSLLVNPTAANPVTILNLPDTTSFYLAVMDSMTGCRNYDSVNVN
                     PVMIDTAMLVLGRTSFCANEPVNASFQLLTNEGSFQWFENNNAVSGATFAVFNPNPAI
                     TSIYQAEIRKDICTDRSRQVIITKLPVPAAGFSISPAEQCLNGPVLISNSSLEPPGVN
                     TNYVWEISDGRSFTNKDLAITFSTPGRKNILLIATSADGCWDTAAQYFQVLDRCGVFV
                     PTAFTPGDDGKNDFFRPYFLGAYRLKRFAVYERNGSLVFETKTIGDGWDGRIKGKMLT
                     TSVLVWMLEYEDDNGKVVFQKGTVTLIR"
     gene            complement(17945..>19876)
                     /locus_tag="PK977_02490"
     CDS             complement(17945..>19876)
                     /locus_tag="PK977_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF17000.1"
                     /translation="GMGYHTPAVADKDYPANDALLQILTNDPSGILYKKLVETKMASK
                     LYAYAQTLHDPGFSYFMVEVPMDKNLDSAKQVLLSTMDDLSSISLTEEDLNRARNTIL
                     KYVEDATSKTTDFAIALTEFVGAGDWRLWYLYRDRIEKLTLDDVKAVAKKYYKPSNRT
                     YGVFIPDKNPERVVVAETPDIQKLLNGYKGKEAVAQKENFENTIENIKRNTEYGTLPN
                     GAKYALLEKPTKGDKINASMILRFGDENGLAGKSEIGAILASLLKTGTTNRTKKQISD
                     ELDKIKTDISFAGGAGSLTITMNTDKQNLPAALSLLEDMLMNPRFDSSEFEKIVIDRK
                     ANYETNKSDPQTIAGERLGKLITNYPKGHPYYAATTEEALEDLSKVKLSDVRKYYSDF
                     YGANNSVSSFVGQLDKKKISGFLENTFGEWNSKSAYKEVEPKYFESKKTTEAINTPDK
                     TNAMLFAAINLDISRKHPDYPAVVMANELLGGGAFLSSRIPQRLRENEGMSYGAGSFL
                     DVQYKYNTGNWGIYAFFNPSYKGRLDSALHQEVDKAIKSGFTQDELSKSIKSWEEQNR
                     TSLGDNGNLAGIIRSFLQNDRDLLQYTEFENKIKALKLDAVNNALRKYFDKSKLVMVY
                     GGDFEKGKATDVKAEKKGF"
     assembly_gap    19877..19898
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19899..>20117)
                     /locus_tag="PK977_02495"
     CDS             complement(<19899..>20117)
                     /locus_tag="PK977_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF17001.1"
                     /translation="KADNLRAFYKKYYQPDNAVLIIAGKFDEKKALQYIQQYFSPIPK
                     PTRMLQPTYTVEPPQDGERKVELKRNGD"
     assembly_gap    20118..20147
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20148..>20502)
                     /locus_tag="PK977_02500"
     CDS             complement(<20148..>20502)
                     /locus_tag="PK977_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF17002.1"
                     /translation="TGMAHLLEHMLFKQCKKFTDIKKAIADKGATANGTTWYDRTNYY
                     EILSASDENLRWALDMEADRMVNSKILPEELAKEFSVVRNEFEIGENYPGSVLNERVL
                     SSMYLWHNYGKSTIGS"
     assembly_gap    20503..20561
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20816..>21130)
                     /locus_tag="PK977_02505"
     CDS             complement(20816..>21130)
                     /locus_tag="PK977_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF17003.1"
                     /translation="ITVDTLQMLVLKKPGYYLPTAFTPNGDGLNDIVRPYLVGMKSLK
                     SFSIFNRWGNLIFYSTREGEGWDGKSKGIAQDGGVYVWMLEFYTNDNKLVTEKGTITL
                     IR"
     assembly_gap    21131..21288
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21289..>21523)
                     /locus_tag="PK977_02510"
     CDS             complement(<21289..>21523)
                     /locus_tag="PK977_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17004.1"
                     /translation="NENGSPQVNYSWSLQDGNPDVLVKDPPSVTYGTAGTIDVVLRLT
                     ALGCEAYPDSIVKKVQVNKPEPGIRYRDITVPQG"
     assembly_gap    21524..21692
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21693..23126)
                     /locus_tag="PK977_02515"
     CDS             complement(<21693..23126)
                     /locus_tag="PK977_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17005.1"
                     /translation="MSECTRNGHFAYAYVDVNSNCNGAISGTYICPGNPGITLTAPFG
                     FQNYTWYSDNTFTTVLSNTQSLVLNPAPAVGTLFPVIVDPYPGFGCRDTLYADVQVAT
                     APVSNAGPDQQVCIGQPVQIGTTSTPGYNYSWSPAGQVSNPTSSNPFAWVSNATPVEF
                     EVITTDILTGCQSRDTTIMTGQSVDTAMVVTGKTTYCAGDANPGILTIGTIQPVTIQW
                     YDGVSPIAGANGITFQPTTSGNYWAQLQGAICTDSTRIVPFTINPLPVANAGPAASVC
                     SNQQIQLGTAPDPANTYTWSPAAQVSNPAIANPMAWSVGTTPQQFIVQVVNAATGCSA
                     SDTTLITGLVVDDAILLTGKKDFCDGDPAGGVLSVNSSLVAVQWYNGNAAIPGATGAS
                     YQPLVTGNYWAQVSDLGCTDSTLTIPFNIYAIPVAGFTASSDTGCVTNNSFTFTNNST
                     VSDGSAMSYLWQFSDGTTQSVTDAVKTF"
     assembly_gap    23168..23285
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23286..>23641)
                     /locus_tag="PK977_02520"
     CDS             complement(<23286..>23641)
                     /locus_tag="PK977_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17006.1"
                     /translation="GGLNTITWTGTGPNPTLHRIITAPGGRDNFGNFPELCPNGSGFT
                     AKIGNETASVTGGIGKEASGISYTYTIPASTTTFSVLFHYAIVLENPNHIPEEQPRFR
                     ARIRDLSTGNTIPCVT"
BASE COUNT         6667 a         4753 c         4606 g         6591 t
ORIGIN      
        1 caaaaattag aattcagata ttcaggagaa aagtacgaag acaaatattt ttatgtacta
       61 aatcaaatct gtattttaag ccaatgaaaa caaaaaccga cagctttgct gaaaagggtg
      121 ttgtaagaaa ggttattgct gcttcctccg ttggcaccat gatagaatgg tatgattttt
      181 acattttcgg catgctggcg aaaaccatca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgtgtgc
      421 cgggttataa aagcatcggt attgccgctc ctattatagt actattatta agattgattc
      481 aaggtctagc cctcggtggt gaatatggcg gcgccgctac ttatgtggcc gaacattcgc
      541 ctgctgaaaa aagaggtttc tataccagct ggatacaaac cactgcaacg ttaggattat
      601 ttgtagcgct gggtattatc atgctggtca agctgaatat gcctgatacc gctttcaatg
      661 ctgaatgggg tggctggcgt taccctttct gggtttctgt tgttttggtt ggcatttcca
      721 tttacataag aatgaaaatg tcggaatcgc caatgttttc caaactaaaa gcggagggaa
      781 aaacatcatt gaatcctatt acagaaagct tccggcataa agccaacttc aagatggtac
      841 tgctggcttt gtttggcgct gtgatgggtc agggtgtggt ttggtacacc ggccagtttt
      901 atgcacagaa ttttttagaa acgaaatgca atttagaatt tgaacaaagc aggaccatta
      961 tgctttgggc cattgccttt gccacacctt tctttctgtt ctgggggtgg ttgagtgata
     1021 agatcggaag aaaatggatc atgatggcag ggatgctgct ggcgatactt acatacaggc
     1081 ctatatttag cacttttttg agtgatactg atagtggcaa atggaaaaat ggtcagacga
     1141 catttataaa agaagaaata gaaaaaaagg tgagtgacgg cgatactatt ttaaaaatta
     1201 cctcccactt taaaacaaac aatggcatta aatacagcaa tgttttagtt gatactgtca
     1261 aagcgggaac ctccaccaaa aaacaagtaa taaaagccaa tttgaactca gcagacctga
     1321 acaagcacct ccctcctgca ttattctgga aatttattgg ccttgttttt attttaatat
     1381 tatacgtcac catggtctac ggccccatcg cttcattcct ggtggaaatg ttccctacca
     1441 aaatccgcta taccagcatg agcctgccct atcatattgg caacggtgtg tttggtggtt
     1501 tagtgccctt catcggctta ctgctcacca ccacataccc ggccgatccg ctagtaggct
     1561 tatggtaccc gatcggcgta gccctgcttt gccttatcat cggtagcctg tatttagaaa
     1621 ataaaattga taaaaacgta atggattaat atgagtgcat taaaaaaata ttttggcctg
     1681 ctattaatgc ttatagcccc ccttattata taccaattag tacatggggc catttcgcat
     1741 attgacccgg ccggaaaaaa ggatatcaat agtcctgtag tctggatcat aatcataacc
     1801 gtttttaccc ctattgctat cggactggtc atttttggct ggtatgcatt ccggggagag
     1861 tatgatcacc tccctcacaa atctaaagag ctgtaaatca gtcagaaaaa attttagaaa
     1921 agttaaacat tcccgttttg cagatgttat aaaggcnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgttgatga agaaaaaaat aataccaaag
     2101 aagactctgt taaaaatcta aaaacgtatt gttcttcagg taactttctc gttttattgc
     2161 ttagcgcaca atccatcagt cactttaaca caactgaacg attagctgtt tcaccttacc
     2221 ttggcaacca taccccgcct cccaattact gttaaagacc ttttctaata actacaattg
     2281 ctgtcacact gatagcagac acacattagt acctaaactg aaattaatat ttcataaata
     2341 atttatgtca gctcaactta acccggtaac aaggatactt aaccttgtcc ggctggaacg
     2401 aaaagaaatc ggtgcaattt acttctatgc catcctgggg gggctgattc agctttctct
     2461 tcctgtaggt attcaaacca ttattggatt catattggga gccacaatgt cgacctcctt
     2521 aaagattctt atcatcctgg ttgtaggagg tgtgttggca ggcgggctgc tgcaaataaa
     2581 ccagatgaag atcattgaaa aaatacaaca gcggattttt gtaagatttt catttcaaat
     2641 tgcagagaga atacccaaat tagacctgaa acaagctgac agttattatt tacctgaatt
     2701 ggtgaatcgt ttttttgata ttacagcgct gcaaaaaagt ttatcaaaac tactgctgga
     2761 tgttcccgga gccagcatcc agatattgtt tggtctgata cttttatctt tttatcatcc
     2821 tttattcatt ttgttaagtg tattgctggt aatcgtatta tcagttattc tttattatac
     2881 cggaaataaa gggctggaaa caagtttgct ggaaagccgt cataaatatg gtgttgctgc
     2941 atggctggag gaaatggcta gagtcatcaa atctctaaag ttttcaaagg gatcccattt
     3001 tcatatgcag aaagcagatg aacgagtttc ggcctatctg caatacagaa cctctcactt
     3061 cagcatactc ctgtttcagt ataaaatgct ggtgttattt aaaacactta tcacggctgc
     3121 catgctggta attggttcgt ttttgctnnn nnnnnnnacc agcaattaaa tatcgggcaa
     3181 tttattgctg ccgaaattgt aattattaca gttattaatt cagttgaaaa attaatcaat
     3241 aacctggata gtgtttatga tgtgctgact tcagtagaaa aaatcggtaa aataactgat
     3301 aagcctatag aacatagcgg aagccttccc atgaaggaag gaaagtccgg ctataaaatt
     3361 gagatgtcca atatcagttt tggctacaat gaagataaac gcatcattca aaacctgtcc
     3421 gtaaaaattg aaccgggaca aaaagtctgc attatgggtg ccgaaggttc cggaaaatct
     3481 acagtattga ggctaatgac cggcgcttat acagaatatg atggaaatat attgattgat
     3541 aatataccta taaaaaacta tgatattgct tcattaaggt catacaccgg tatcttcctt
     3601 agtcagcagg atattttcca gggaagcctt gaagaaaata taacaatggg caatgaagaa
     3661 attgaccgga ccgaagtaat agccttattc gaaaaagtag gcctattggg attccttaca
     3721 tcattgaagg atggcttcga taccgagctt gatcctacgg ggcatcgctt acccaaaaac
     3781 gttgtacaca aactcttatt agtaagagca cttgttaata atcctagatt attattactt
     3841 gaagatccct tctccggcct tgaagaaaca ttcaaactgc agatacaatc gttgctacta
     3901 gacaggaata accctgctac tgtggtaatc agtacgaatg atgaacagtt tgcacaacag
     3961 tgtgataaga ttgtccggat aaattaataa aaacgagttg cataccataa tacaacttaa
     4021 aaaaaatgaa agagaaagaa ctaaacgcat acagccagat atatcgccac aataaaacga
     4081 gcaaagtaaa atattggttt tacgggatac ttgggtttat cctgttattc ctttttctgc
     4141 cgtggacaca gaacatccgt gctaggggtt cggttactac cttaaaaatg gaacagcggc
     4201 cacaggaaat gaatacgatc atacccggaa aagtagtgaa atggtatgtt aaagaaggcg
     4261 attatgtaca ggcaggtgac accattattc agctttcaga gattaaagat gaatacctgg
     4321 atcctaacct gatcaacagg acacaggagc agctatcggc aaaacaaatg acaattgatt
     4381 attacaaaaa taaagtagac gtaacccaaa cccagatcgg tgctttaagc agtggccgtg
     4441 aacttaaact aaaccagctg gaaaacaagt tagcccagtt acggctaaaa gttcagagcg
     4501 acagtatgga ggctattgca gcgaataatg atttcaatat tgccacacaa caatacaacc
     4561 ggcaaaaagc aatgtacgac agcggacttg tttcacttac acaattggaa caacgcaacc
     4621 agtcttacca gaatgctctg gctaaaaaaa taagcgctga aaataaactg gccaacagca
     4681 ggcaggacct taccattaca aaaattgaaa tgaacagtgt acagcaggaa tacctggaaa
     4741 aaattgccaa agcccaggga gatcagttcc agtccatgag ccagatagcc tcaggccagg
     4801 gcgatattgc aaaactggaa aaccagtaca gcaattactc aatacgaaat ggcatgtact
     4861 atattacagc accacaaagc ggacaggttg tgaaagcaaa aaaggcaggt attggtgaag
     4921 tcgttaaaga cggggaaatg atcgttgaaa ttgttcccga taaaattcag tatgcagttg
     4981 aactgtttat caggccgctt gatctgccgc tggtggcacc gggacaaaaa gtgcaatttc
     5041 tttttgatgg ttttccagcc attgtattta gtggctggcc gaatgcttcc tatggtactt
     5101 tccgtggcaa agtggtagcc gttgaaagct ctgtgagttc caacggaaaa ttcaggatac
     5161 tggtagcaga agatgcagat tataagcctt ggccgaaaca gttacgagta ggtacaggtg
     5221 cacaaggaat ggctctttta aaagatgtac cggtctggta tgaactctgg cgaaatatta
     5281 atggtttccc gccggattat tacaaacccg ctacaacaac taataagaat gaaactaaaa
     5341 aataaacaga tattaatagc aggcctattt gctctttgct tttctgcaaa agcacaaaac
     5401 aaccaggaga aaaccctttc cccatcccag ttagtagaaa tggtaagaaa attccatccg
     5461 gttgccaaac aagctgatat taatattgac aaagccaaag ctgatataac aattgccagg
     5521 gccggatttg atccgtattt ctataactct tccgctcaaa agacatttga tgggacagac
     5581 tattattatt acaaccgtcc acaggtaagc attcccacct ggttcggcgt agaaatatca
     5641 gggggcagag aatatctttc aggcagccgc accaataatg aagaaaccct gggtgaaacg
     5701 agttactttg gtatcaccgt tcctttggct aaaaacctgt tgatggacaa aaggagagcg
     5761 gcattgaaaa ctgcgaagat attcagagag gcttctgtta ttgaaaagaa taatattctg
     5821 aataacctga tgctcgaagc attgaatatg tactgggaat ggacagggca ataccaactg
     5881 tataacatcc taaccaatgc agtaaaagta aatgagcaac gactgcaact ggttcgcatt
     5941 gcttacaggc aaggggaccg gccagctatt gataccactg aagccctaac ccagctacag
     6001 agctttcaac tcttgcagtc gcaggcatgg ctgtcctttc aaaattcagg tcttgaactt
     6061 tccacttatt tatggactac cgaaaacctt cctttctcac ttccggaaga tgtcattccc
     6121 gatgaagaat cacggggatt gaatacgata aaattgaacg taccagaatt aaaccaatta
     6181 ttggatgcag ccaggcgcaa tcatccagaa ttattacaat ataatttcaa gttagacgca
     6241 ttggatattg agaaaagact gaaattccag gatcttttac cttccgtcaa cttcaggtat
     6301 aatcaactgg gtaaagggta tgacatcctg aaaacagcaa ccggaccttt atttgagaac
     6361 aactaccagt atggtttaag tattggcatc cctcttcgtt tctcgcaggg aaggggcgaa
     6421 tacaaaaaag cgaggctcaa gatcagcgaa acaaaactgc aacaaaatct gaaacaatta
     6481 cagatagaaa ataaagtaag aagttattat aacgaactca atacccttcg tcagcaaatc
     6541 agtttgcagg aaaaagcgta tcagaatttc atgatactgc aacgggggga ggagacccgt
     6601 tttcaaagcg gtgaaagttc attatttctt atcaacagcc gtgaaaacaa gagcctggaa
     6661 gcgctgcaaa aacttactga attaaaagca annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnaga acaatcaact atttaaactg ggctgcaggt atattacgct aattgttagc
     6781 gttttgtaat atatgagctg ggggtttcag aagtgaccta tataagttat attcgcaccc
     6841 tgatggctaa agaacatatc tattactcct tataccataa aagcagcagt atcctgatga
     6901 tacttgcatt gctatggctt actgtgagta ttccatatgt ttttaatgca caaaaacaac
     6961 tacttagcca ggaaaaacaa caacctgtac ctgtcaattc acagcaggaa gaagaagcag
     7021 ctaatccttt tgccagtaca actgaagaaa aaacacctgg ttcaggaaac acactttctg
     7081 aatttctgca tgaaattgag ttgcttaagc atccttcccg aaatcttacc cgatacttca
     7141 tgtatatcag atcttctctt tacatagcct tccacggcga actattggtg ccccctccaa
     7201 actgttaagt ttagtttttc attctttctt tctataatct attaacctgt nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn naaagtttac caaacgactt tgctgcttct attgtagtat
     7381 tcctggtggc actaccactt tgcctgggaa tagcgcttgc ttccaacgca ccgttattca
     7441 gcggactgat tgccggaata attggtggca ttgtcattgg tttagccagc cgctcccacc
     7501 tgagtgtaag cggccctgcg gcaggtttat cagctattgt agccggcgct attgccttgc
     7561 tgccctcatt cgaagccttc ttgctggcag tagttattgc cggtattttt caaatagccc
     7621 ttggcttcgc caaatccggt attatcggcg attatgttcc aaacagtgtg attaaaggga
     7681 tgcttgctgc catagggctt atcctgatat taaaccaggt accgcatttg ctgggcgatg
     7741 attcgcactt tgaatctgat gaaggtgttc cacaggccaa gggaaatatt ttttccaatt
     7801 tcatatttgc atttggcaat gtaaatacca cagctctgct tatcgggggt gtatgtcttg
     7861 tcttttattt tttatgggaa aaattggtat cgggcaagaa aggactcata cggtttgtac
     7921 ctgcgccgtt gatagtggtt atccttgctg taatcatcaa ttattttttt cagtaaaaag
     7981 gcaccggcct gaacggcgaa caccttgttg ttatcccgca ggcctcttcg gccaatgagt
     8041 tcttttcttt cttttcttca ccggactggg gcatgataat gaataaaaat gtttggatta
     8101 ttgccattac attagccctt gtggccagtc ttgaaagttt attaagcata gaggctattg
     8161 acgatctgga cccttaccag cgggtaacac ccaccaaccg ggagttaaaa gctcaaggct
     8221 tcggcaatct tttttctggt ttattaggcg gattgccggt taccagtgtg attgtgcgtt
     8281 cgtctgccaa tgttaacgcc ggggccaaaa caaaaatgtc taccatttat catggaatct
     8341 tattactagt atcagttgct tttgttcctg ctatcctgaa tatgatacct aaaacagcac
     8401 tggcagccat ccttattttt accgggtata aattagcaaa gcctgcacta ttcattgctt
     8461 tttataaaaa aggatgggat cagtttctgc ctttcgttat tacaatcgtg gcaattcttt
     8521 caactgatct cttaaaagga gtaattatcg gtattggcgt agggttattt tttgttatca
     8581 gaagtaattt caggtcttca gtagtagtgg taaatgataa taaccggtac ctcatccgcc
     8641 ttagaaaaga tgtatcattc ctcaataaac ccatcatcaa aaagaaactg gaagaagttc
     8701 ctgaaaattc ccatgtgctg attgatgccg gcagggcaga ttttattgat aaggacataa
     8761 ttgaagtgat tgaagatttt atcattcatg cccccttgtc taacattaca gtagaagtaa
     8821 aacgcagttt gtataaagat cagggcttta atcgtaaatt agttcctccc gatgcaaacg
     8881 gccggtatgt gaggacacat gaaaaaacta tcaaaccagc tacagaaatt taattctaaa
     8941 ccgtaataaa taaccagcaa tgaaatcata cgaaaaactt ttactagaaa acagggcctg
     9001 ggcatcggaa aaagtttctg aagatccaga tttttttaac cgcctggccc accttcaaac
     9061 acctgaattc ttatggatcg gctgcagcga cagccgggta ccggccaatg aaataactgg
     9121 tacacaaccc ggagaaatat ttgtacaccg caatgtggcc aatatggtgg tacatacaga
     9181 ccttaacctg cttacggtat tggattatgc agtaaatcac ctgaaagtaa aacatgtgat
     9241 tgtatgtggc cattatggtt gtggtggtgt gaaagcctct atgacaaaac ataatttcgg
     9301 tatcattaat aaatggctta gaaatattaa agatgtttac cgttttcaca gggaggaact
     9361 ggaagaaata aaagatgaag aggcaagggc tgacaagctg gttgagctaa atgtgcagga
     9421 acaggttatg aacctggcca agacatcaat tgtgcagaaa gcatgggctc atcatcagcg
     9481 accgcattta cacggctggg tatatggatt aaaggacgga attatcaaac ctgtatttga
     9541 gatgcctgca ggcacacata tagatgacct ttatgagttt gatgatctgt aagaaattaa
     9601 ttatagtgct tatgagcctc tttaatcagc ctttcattca tagcttttcc aaggccagcg
     9661 tcaggcattt tttcggctat aataagatca aagtttccct tatctaattc atataaaact
     9721 gaataaagat gctgagcagc ttcttccaga tcaccagagt tgctcagcat tattttttct
     9781 ttaacgggaa cagaaggaaa gttctcagtg aagcaaatgg cagctatatt tttctgaaac
     9841 cgcaaccttg tgaaatcact attacgattc atcaaccaaa gtggcgtctt tggagaatag
     9901 tgctttttaa acatgccggg atgcaggtga cctgaggatt tttgttcact cataggtttt
     9961 tgtaaaacgg attcannnnn nnnnnnnnnn tcctcagtaa tcgcaccatg tcgtaaaatc
    10021 tttatctgtt tttccacaaa agaaacaatt gttgactcaa tacccttttg acacctgccc
    10081 ccatccaaaa tatacgggac ttcatttccc agcatttctg atactgcttc tgctgtaaca
    10141 gggctgatgt agttaaattt gttggcactc ggtgcagcca gcgggaattc taaaagcgac
    10201 agtaattcaa gtgtaagcgg atgtgaaggc actcttacgg caacggtttg ctgtccggca
    10261 gtaacaatat ccgggataat agctgccttt ggtaaaatca tggtcagggg gcccggccag
    10321 aatgattttg ccaatatttc agcctcggca taaaactcct tcaccaatga ttttacctgc
    10381 tgaatattta aagcatgtac aatcaatgga tttgataaag gcctgttctt tacatcataa
    10441 attttctgaa tggcatcaac gttataagca tttcccgcca gcccgtacac tgtttctgtt
    10501 ggtatagcca ctacctctcc ttttctcagc cattctgctg ctagttgtac actggagcct
    10561 ttcatgataa aatttatttt atttattgcc cctggcccag tgagtaaccg gctttcccat
    10621 caaaattgta caggttttcc gttttaataa tatcaaaacc ttcttgtgaa agttgcccgc
    10681 aaagctcaag tacttctgca tatttacccg gagcatcagc aacaaaacga catcgccaat
    10741 ggtctgtaca gaaagtttcc ggtatgccgc caggataaac ctttaccccc cgattggtaa
    10801 tcatgttcaa tgcaaaatga ccggccttat atttactccc ttgatttccg agtacctccg
    10861 gatttctgtc gtctttattc cagtgtaaaa aaacatctac tccaaccaac tccttttttg
    10921 aaattttcgg aaccggcatc gatatagtga tttttttatc tccttccatt gtatatgtta
    10981 ctgccggtaa ttgttgtgga acttctccca gtctttcgat aaccgctttt gtaaataatc
    11041 ttgtacctac ctgcacttta ctctttcctt cccgaaatat atctcttgta tgaataccgt
    11101 cttcaattgt ctttaaccag gcattgtgaa tcttttgagc cacatcgtgc tgacctatat
    11161 acaccagcat ttgcactgca cccagtaaaa taccggaagg attagcgaca tcctgtcccg
    11221 ctatatccgg tgctgatccg tgaatagctt caaacatggc acatgctgaa ccgatattag
    11281 cagagccacc caggcccacg gaacctgtaa tttgcgctgc cacatcagat aaaatatctc
    11341 catacaaatt gggcataacc acaacatcaa atatttcggg agtatcagcc agccttgctg
    11401 caccaatatc aactatataa tgttcctttt ttatctcagg gtattcattg ccaatttcat
    11461 caaacatcct gtgaaataaa ccatccgtta ttttcatgat attatccttg gtaaagcagc
    11521 tcaccttctt gcggccataa accctcgcat attcaaaggc aaaacgaaca atcttttcaa
    11581 tgcccggctt cgatatcaat ttcaggcatt gtgttacttc atctgtttgc tgatgttcga
    11641 taccggcata caggtcttcc tcattttcac ggataatcac cacatccatt tcaggatgat
    11701 gtgtagtaat gaagggatga taggacatac aaggcctgac gtttgcaaat aatcccaggg
    11761 actttcgtat tgttacattc aggcttttat agcctgtacc ttgtggtgta gttataggtg
    11821 cttttaaaaa aacctttgtt gacctgagaa cctgccatgc ctcaggagat atccctgaag
    11881 tatgcccttt caagtactgt ttttcaccaa cctctatttc ctgaaaatca atcctggcac
    11941 cggcagcctg caaaatttca agagttgatc tcattatttc cgggccgatt ccatccccgt
    12001 atgctatggc aaccggggtg ttcaaagagt gctgcataag attatttttg ggcaaaaata
    12061 gtaatacatt ttaaaataat agtattacta ttataaaaaa tagtaaaatt atttttccta
    12121 cttttgtccg aaataaggaa aatggaactc gcagttcaga taaaaatgaa tgaaaaatta
    12181 tttctgcgaa acccggagtt atccgaactg ggcagaaata ttattaaata tagtgtggta
    12241 atgatccggg agaaaggttt cgaagacttt acatttaaaa aactggccca acgtattggc
    12301 actacagaag ccagcattta ccggtatttt gaaaacaaac accgattact aatatatata
    12361 gttgcctggt actggagcta cctggaatac aaagtagtat tctatacgca aaacctcgca
    12421 aacccggaaa acaggctaaa aaaaataata gaattattgg ctaagccggt tactgatgat
    12481 tttagtactg actacataga tgaaaaactt gtgtacgaag tagccgtttg ggaagggtca
    12541 aaagcatatt tgacgaaaca tgtcagtaaa gacaataaag acaggctctt taaaccctac
    12601 aaggatctgt gtggaagaat tgccaacatt attgctgagt ttaatcccag gtataagttt
    12661 ccccgttctt tatcgagtac attactggaa atggcgcatt accagcgttt ttttatgaat
    12721 aaccttcctt ctctcacaga ttttggagag gataaaagtg aagagcggct ttaccagttc
    12781 ttataccagt tggtttttga tagtttaaaa aaataaagaa aagccagatg gattataatc
    12841 tcatcctaag taatcttacc aatcctaccc tgcttttctt tttattaggt gtaattgcag
    12901 ctctgataaa atctgatctt gagatcccgg tacaaagttc aaaattcatt tcactttacc
    12961 ttcttttctc cattggcttt aaaggaggta tggaattatc gcacagtggt attaacaagg
    13021 aagtagccat ttcactacta cttgctattt tctttgctgt taccgttcct gtttacagtt
    13081 attttatctt acgacggaaa ttagacgtat ataatgcagg cgctatagca gcaacatatg
    13141 gctctgtaag tgctgttaca tttgtaacag cctgcagctt tttggcagat aagaatgttg
    13201 aattcggcgg acatatggtg gctgccatgg cactcatgga atcaccggct attattatcg
    13261 gaatgattct gatcagaaaa ttcagttctg aaaaacagca acagcccttc agccacatta
    13321 tccgtgaagc atttagtaat ggctctgtcc tgatgatact cggaagcctt gtcatcggta
    13381 tagtagcaga tgaaaaacag gcagaaggta ttaagccctt taccagtgat atcttcaagg
    13441 gcttccttgc tgttttttta ctggatatgg gtattacagc tacaaataga ataaaagcat
    13501 ttaacaacgg tggtctgttt cttgctttat tcgggattct gctgcctgta ttcaatgcct
    13561 gcctggctat attttttgca cacagccttg gagtacatga cgggaatgca ttcctgattg
    13621 ccattcttgc atccagcgca tcctacattg ccgttccggc agcgcttcgt cttgctgttc
    13681 ctcaggcaga cccggggtat tatataccaa tggctttggc tataacattt ccattcaaca
    13741 ttgtagtcgg aattccgtta tattatggat tattgtcagt tttaaaattt gaaagcatat
    13801 gaaatgtcct tcttgtaatg aaactttagt gatgacagaa cgccagggcg ttgaaattga
    13861 ttactgtcca aaatgccgag gtgtttggct ggataaaggt gaattagata aaataataga
    13921 aagagccgca ggtgagcagc cgggaaacac ctcaaagagc aaaaaacaat gggatgatga
    13981 cgatgatgat gacgacaatg attttaaccg gaaaggtggc cgcagaaaag gtggtttcct
    14041 tggagattta tttgacttcg gataaaaatg attatggcca acacctggtt atggataggg
    14101 attaatgtat ttgtgttatt gatgctggag cttgatcttg gtgtctttca caggaaagaa
    14161 cacaaagtaa cattcaaaga agcgatcagc tggtcggtcg tttgggtaac attggctctt
    14221 attttcaacg gctggatcta ttatgaccag ggcaaagaca aagcacttga atttctgacg
    14281 ggttatctta ttgaaaaatc gctcagtgtt gataatattt ttgtatttgt actgctgttt
    14341 tcttttttca aggttccgga acaataccgc caccgggtat tgttctgggg cgtactgggt
    14401 gccttattga tgcgtgccgg tttcatagca gcaggggcag tattaattga aaagtttcac
    14461 tggatcattt atgtttttgg agccttcctg gtgtttaccg gctataaaat gctaaataaa
    14521 aaaacagagg atatgcaccc ggaagacaat ccgctggtaa ggtggtttgt gaaaaaaggg
    14581 aaagtgacca gcgattatca tggcaaaaat ttctttgtac gcattaacgg gcaaaaactt
    14641 gccacgccat tatttctttg tttgctgtcc gtagaattta ccgacctgat ctttgctgtt
    14701 gattctatcc cagccatttt tgctatcacg aaagatccct ttatcgtata tacttcgaat
    14761 gtatttgcta tccttggtct ccgttctctt tattttgcac tcgaaggtat tattacccgg
    14821 ttcccctacc tgcgttatgg ccttgcctta attctaattt ttatcgggct taagatgctg
    14881 gcagtggata tatataaaat accagttgtg gcatcgcttg cagtgattgc cgttattctt
    14941 ttaacttcca ttttttggaa agggaaaaaa caacctggat aatgagttcg ctttaccaac
    15001 attttattcg atttcccctg caaggcatat aactttgtca catgtcaatc gaagtcaaca
    15061 acctgctgaa agaatacggg gaacaaaaag cggtcaataa tatttcattt aagatcaata
    15121 aaggagagat cgttggtttc ctggggccaa acggggccgg taaatccact accatgaaga
    15181 tcataaccgg ttatcttcag cagactggag gagaagcttt tgtgtgcggt attaatgtgg
    15241 ctgaacagcc ccttgaaaca aaaaagaaaa tcggctacct gccggaattg aatgccttgt
    15301 attatgatat gtatgttcgt gaatatttag gttttgttgc cgaagtacat gcaatcaaag
    15361 atccaaaatc aaaaatcgaa aatgttatag atactgtcgg cctcaccgtt gaatcaaaaa
    15421 agaagatcgg gcagctttcc aaagggtata aacaacgggt aggcctggcg gctgctctta
    15481 ttcatgaccc ggaagtattg atcctggatg agccaacctc aggacttgac cctaaccaga
    15541 taatagaaat cagggaagta ataaaaaaac aaggacagaa taaaacagta ttattctcct
    15601 cacatattct gcaggaagta gaagctattt gcgacagggt cattattatt aacaaaggtc
    15661 agattgtggc tgatgacaaa cttagtaacc tgcaaaagag caataaggag agtcatattg
    15721 taatagtaca gtttaaggaa gcaattgata agaaccggtt aaccggaatg aagcaggtat
    15781 caaaaattga ggaagcagaa cataatttgt tcaagttgca tacatccgat cccgaggcag
    15841 ttcggaagca aatccttgaa ctggcattac aacagcagct caatattctt tcattgcaaa
    15901 gtgaaagccg cagcctcgaa gatgtgttcc ggtctttgac acacaactaa ctattcctag
    15961 cgaatcaaag taactgttcc tttttgaaag acaacttttc cgttatcatc ctcgtattcc
    16021 agcatccaca ctaatacgga ggttgtcagc attttccctt tgatgcgtcc gtcccatcca
    16081 tctccaatag tctttgtttc aaataccaaa gatccatttc tttcataaac ggcaaaccgt
    16141 tttagcctat aggctcctaa aaaataagga cggaagaaat cattcttgcc atcatcacca
    16201 ggtgtaaacg cagtaggcac aaaaacacca catcggtcaa gcacctggaa atactgcgca
    16261 gccgtatccc agcatccatc agcagatgtg gctatcaata aaatattttt tctgccggga
    16321 gtactgaacg taattgcaag gtctttgtta gtaaaacttc ttccatcact aatctcccat
    16381 acataatttg tatttactcc cggtggctcc aaactgctgt tggaaattaa caccggcccg
    16441 ttcaggcatt gctcggccgg agatatagaa aatcctgcag cgggcacagg cagctttgta
    16501 atgattacct gccgtgatcg atctgtacaa atatctttcc tgatttctgc ctggtaaata
    16561 cttgtaatag ccgggtttgg attgaaaact gcaaaggtgg caccgctaac tgcattattg
    16621 ttttcaaacc actgaaaact tccttcattc gttaaaagtt gaaaagatgc gttaactggc
    16681 tcattagcac aaaaagaagt tcttcccaaa accagcatag ccgtatcaat catcacaggg
    16741 ttcacattca ccgaatcata attcctgcat ccggtcatac tatccattac agcaagataa
    16801 aatgaagttg tatccggcaa attcagtatc gtaaccggat tggctgcggt tggatttacc
    16861 aaaagcgaag gtggtgtcca tgaatatccg tacccttcca aaggatttgt tcctaactgt
    16921 gcaaactggt tcttgcaggt ggttacatca ataccagcgt ttgccaccgg gagagtaaca
    16981 gtttttaata ccgcaactac agtatccggg caaccaaatc cgggataagg accaacaact
    17041 accggaaagg aagtgccggc aggtggtaca ggactaagcg ttagtgcctg cgaagaagat
    17101 agtactgtcg aaaatgtttg atctgcatac cagctatacg tttgaaatcc ataaggagcc
    17161 aacagggtca cttcatccgc atccgcacaa acctcacttc cggtaacagc gccaccgcat
    17221 gaaccgctca catcaatata ggcgtatcca aaatgccccc tgaatgtgca gtcactggtg
    17281 ataaactcaa gtcgaatcgt tttaccagcc aaaccagaca ggttaattgt aacaggcgtc
    17341 cagtctttat aaataacagt tggatctaca gtagaaacag tgaagccggg caaggaagct
    17401 gaagctgtaa aatcaaaatt ggcacaattg atcacttcat tgtcggttac attatatacc
    17461 cttgccctga agcggggttg ttcatccgga ttgtgctgag gatcctgaaa aaccacagca
    17521 taataaaaaa gaatagaaaa taaagttgct gtatcgggaa tactgaaggt ataaaacatg
    17581 ccttcagcgc ttatattctg aaaatagttg ttgcccaatt gtacgctata ggcactgcca
    17641 ttcggtgaag cctgtgagaa atttccatag ggatcaagcc ctgccgtagc ccttggaatg
    17701 atttggtgat ttagcgggtt aacaccactg ctctcccatg ttaccgtatt gacacctgca
    17761 atactttcta cccttccggt cttacattcc cagttattta aatcacccgt ttcaaaatca
    17821 atatttgagg gacactgaga accctgacaa aatactgcca gcgtattgac cgcagccaga
    17881 agcgataaca gtatatgttt gctgtaagcc tgcatatatt tttcagcagc ctttatctca
    17941 tgaatcagaa gcctttcttt tcggccttta catcggtagc cttacctttt tcgaaatcac
    18001 ctccgtatac catgaccaat tttgatttat caaaatactt tctgagtgca ttattaaccg
    18061 catccagctt tagtgctttg atcttgttct cgaattcggt gtattgaagc aagtccctgt
    18121 cattttgtaa aaaagaacgg atgataccgg caaggttacc attatcaccc aaagatgttc
    18181 tgttttgctc ttcccagctt tttatcgatt tactcaattc atcttgtgta aagccagact
    18241 ttattgcctt atccacttcc tggtgcaagg cggaatccag tcttccttta tacgaaggat
    18301 tgaaaaatgc ataaatgccc caattgcccg tattgtattt gtattgtaca tccaggaaac
    18361 tacctgcccc atagctcata ccttcatttt ccctgaggcg ctgcggtatc cgggaagaaa
    18421 gaaaagcgcc accgcctaat aattcattgg ccattaccac ggcaggataa tccgggtgtt
    18481 tgcgggatat atccaggttg atagcagcaa atagcatggc attggtttta tcaggagtgt
    18541 tgatagcttc agttgttttt ttgctttcga agtattttgg ttctacttct ttatatgctg
    18601 acttgctgtt ccattcacca aaagtattct cgaggaagcc actgattttt ttcttatcca
    18661 attgaccaac aaaagatgaa accgagttat tggcaccata aaaatcactg taatattttc
    18721 ttacatcgct gagttttact ttcgaaagat cttcgagtgc ttcctcggtt gtggcagcat
    18781 agtagggatg tcctttagga tagtttgtaa taagtttacc tagtctttcc ccggcaattg
    18841 tctgtgggtc gctcttgttc gtttcataat tagccttcct gtcaattaca atcttctcaa
    18901 attcgctgga atcaaaccgg ggattcatca gcatatcttc cagtaaactg agggctgcag
    18961 gaaggttctg cttatcggtg ttcatcgtaa tggtcaatga ccctgccccg cctgcaaaag
    19021 aaatatcagt tttgattttg tccagttcat cactgatttg ctttttggta cgattcgttg
    19081 ttcctgtttt caataaagaa gccagtattg ctccgatttc tgattttccg gccaggccat
    19141 tttcgtcacc aaaccgcaat atcatcgaag cattgatctt atcaccttta gttggctttt
    19201 ccagcaaggc atatttggcc ccgtttggta aagtgccgta ctctgtattt cgctttatat
    19261 tttcaatagt attttcaaaa ttctctttct gtgccacggc ctcttttcct ttatagccat
    19321 tgagcaattt ctgaatgtcc ggcgtttcag ccaccaccac tctctccggg ttcttgtcag
    19381 ggataaatac gccataagta cggttggatg gtttgtaata ttttttggca actgctttta
    19441 catcatccaa tgttagtttc tcaatacggt cacggtacag gtaccatagc cgccagtcgc
    19501 ctgcacctac aaactctgta agggctatcg caaagtctgt tgttttactg gtagcatctt
    19561 ctacgtattt aagaatagta ttccgggcac ggttaaggtc ttcttctgtc aagctgatag
    19621 aagaaaggtc atccatagtt gatagcaata cctgtttggc gctgtcaagg tttttatcca
    19681 tcggcacttc caccatgaaa taagaaaaac ccggatcatg cagggtttga gcataagcat
    19741 aaagctttga tgccatttta gtttccacca gctttttgta tagtatccct gaaggatcgt
    19801 tggtaagtat ttgcaacaag gcgtcattgg ccgggtaatc cttatctgca acagcaggcg
    19861 tatgataacc cataccnnnn nnnnnnnnnn nnnnnnnnta tcgccattgc gctttagctc
    19921 cacttttcgt tcaccatcct gcggaggctc caccgtatat gttggctgca gcatccttgt
    19981 aggtttggga ataggagaaa aatactgctg tatatattgc aatgcctttt tctcatcaaa
    20041 ttttccggca atgatcaaca ctgcattatc cggttgatag tacttcttat aaaaagcccg
    20101 gagattatct gcttttannn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc tgcctattgt
    20161 cgattttcca tagttgtgcc acaagtacat actggagagc actctttcat tcaatacaga
    20221 accggggtag ttttcaccta tttcaaattc atttctaacc acactgaatt ctttagccag
    20281 ttcctccggc agaattttag agtttaccat ccggtctgct tccatatcca atgcccagcg
    20341 aaggttctcg tccgaagcag ataatatttc atagtagttg gtacggtcat accaggtagt
    20401 accgttggca gtagcacctt tgtcagctat cgctttttta atatcagtga actttttgca
    20461 ttgcttgaac aacatatgct ccagcaggtg ggccatgccg gtnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacaactat gttcgtttgc
    20581 gattcgtcgg gaataagcaa tacccgcaaa ccgttcttta attcatattc ttttactcct
    20641 tctaccgtag ttatcagttt aggggaggtt tgtgccacta aagaagcagg agaaataagg
    20701 ataaggccga ggaccaacag aaacaatctc ataattgtaa ggttttatga ttcagtctaa
    20761 agtaaaatca atttctgaaa acgggtaacc gcttgtaaat aaccggatgg atgcgtcatc
    20821 gtataagggt gatggtgcct ttctcagtca ccagcttatt atcattggta taaaattcaa
    20881 gcatccacac atatacaccg ccatcctggg ctattccctt gctcttaccg tcccagcctt
    20941 ctccttccct ggtagaatag aagatcaggt taccccaacg gttaaaaatg gaaaaactct
    21001 ttaaggattt cataccgacc aggtaaggcc ttacaatatc attgagacca tcaccattag
    21061 gtgtaaaagc agtcggcagg taatacccgg gctttttcag caccagcatc tgcaaagtgt
    21121 ctactgtaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ccctgcggca
    21301 ctgtaatatc ccggtaacga atacccggct caggtttatt tacctgtact tttttcacaa
    21361 tggagtcagg ataagcttcg cagccaagcg ctgtcaatct caatacaaca tctattgttc
    21421 cggcagtgcc gtaagtaacg gagggaggat ctttgactaa tacatccggg ttgccatcct
    21481 gtaaactcca ggaataattg acctgtggcg agccattctc attnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaagttt taactgcatc cgtaactgat
    21721 tgtgttgtgc catcgctgaa ctgccagaga taactcattg cagagccatc actcaccgtg
    21781 gagttattgg tgaacgtaaa ggagttatta gttacgcaac ccgtatcact gctggcggta
    21841 aatcccgcta ctggtattgc ataaatatta aacggtattg ttaatgtaga atctgtgcaa
    21901 ccgagatcgc ttacctgtgc ccaataattc cctgttacca atggctgata gctggcaccc
    21961 gtagcgccgg gtatagcagc attaccatta taccactgta cagccaccag gctgctattt
    22021 actgaaagca caccgccggc aggatcgccg tcacaaaagt cttttttgcc agtaagcaaa
    22081 atagcatcat ccactaccag gccggtaatg agagtggtat cactggcact gcaacctgtt
    22141 gccgcattta ccacctgtac aataaactgc tgtggggtag ttcccactga ccaggccatt
    22201 ggattagcaa tagcggggtt gcttacctgc gctgccggcg accaggtgta ggtgttggca
    22261 ggatctggcg ccgtacctaa ttgaatttgc tgattgctgc acacagatgc agcgggtcct
    22321 gcattagcaa ccggtaatgg gttaatagta aaagggacga tccttgtaga atctgtacaa
    22381 atagcccctt gtaactgcgc ccagtagttt ccagatgttg ttggctgaaa ggtaatacca
    22441 tttgcacccg caataggcga aactccatcg taccattgta tcgtaaccgg ttggattgta
    22501 ccaatagtca atatacccgg attggcatca cctgcacaat aagttgtttt gcctgttacc
    22561 accatcgcag tatctactga ttgacccgtc ataatggttg tatctcttga ttgacaacca
    22621 gtcaaaatat ctgtagttat tacttcaaac tcgaccgggg tagcattgga tacccaggca
    22681 aagggattag aactggtagg attgctgact tgcccggccg gcgaccatga atagttatat
    22741 cctggtgtac tagtggtgcc aatctgtaca ggctggccaa tacaaacctg ttggtcgggg
    22801 cctgcattag aaaccggtgc tgtagccacc tgcacatcag catataatgt gtcccggcaa
    22861 ccaaacccgg gataagggtc cactatcacg ggaaataatg taccaacagc cggcgccgga
    22921 ttcaaaacca gtgattgtgt gttagataaa acagtcgtaa aagtattatc tgaataccat
    22981 gtataattct gaaagccaaa gggagctgtt aatgtgattc cggggttgcc tggacatata
    23041 taagtcccgg aaatagctcc attacaattg ctatttacat caacgtaagc ataggcaaaa
    23101 tgtccgtttc ttgtacactc tgacacaatg aactcgattt caatcgtcct gccggcaagt
    23161 cctgatannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnacgta acacaaggaa tcgtattgcc tgttgaaaga tccctgatcc tggctctgaa
    23341 tcttggttgt tcctcaggaa tatggttggg gttttctaaa acgatagcat agtgaaataa
    23401 aacagaaaaa gtggtggtgg atgctggtat agtataggta tacgaaattc cgcttgcctc
    23461 ttttcctata ccaccagtta cagaagcagt ttcatttccg atcttggcag taaaaccact
    23521 accgttagga cataactcag gaaaattgcc aaaattatcc ctgccaccgg gagcagtaat
    23581 gatcctgtgt aaagttggat tgggtccggt tccagtccat gttattgtat ttaatccgcc
    23641 a
//
LOCUS       DAORLS010000015        21615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1316_length_21615_cov_2.209647, whole genome shotgun sequence.
ACCESSION   DAORLS010000015 DAORLS010000000
VERSION     DAORLS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21615
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_21615_cov_2.209647"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..>417)
                     /locus_tag="PK977_02525"
     CDS             complement(198..>417)
                     /locus_tag="PK977_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17007.1"
                     /translation="KNDLMQLGICSILVPAKYYELIWVDDIGWPHFKQLKQLPNVNLA
                     ERNFLLKQYVLLYNDRNKLVKPPLHAVK"
     assembly_gap    418..432
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(769..2742)
                     /gene="ftsZ"
                     /locus_tag="PK977_02530"
     CDS             complement(769..2742)
                     /gene="ftsZ"
                     /locus_tag="PK977_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRF17008.1"
                     /translation="MIHFDLPKEKSSILKVIGVGGGGGNAVNYMFSQQIEGVNFIICN
                     TDAQALSNSGIPNRIQLGPHLTQGLGAGANPEIGRQATEESLEEIKRILEVNTKMAFI
                     TAGMGGGTGTGGAPIIAKICKDLGILTVGIVTTPFAYEGKKRQQQAMEGIKNLEQYVD
                     TLLVISNDKLRHQFGNLKMREAFEKADNVLATAAKCITDVINSTGQINVDFADVCTVM
                     RSGGVAILGNAEASGEDRAQKAIEEALNSPLLNDNDIRGAKWILINITSATGEHEFTM
                     DEVEVIQNYLLTQAGENTDVILGMGYDSNLGDKLGITLIATGFEHKDPFAKPVAKVEE
                     PKKEEKIVMVLGQTNEEEKKTVAIKEEALMKEKEMEVMETALKPVPAEDYLMPKLVEE
                     IPVQPAPVIDLSDIKAEEEPLVVHFELKPDTEILLSNTTGEPVGTSTVGKLSIVGKDD
                     SINSTEQNSSVPQQAIKETDAELIVSRKDSTSSTTPVSAASGGYLARPSNIYAASQAE
                     DTTSKRAAEEPVLSAKTTASEEEEELLELQMQIVERDDIPAADMPLAHQAQSPLTAEV
                     EDPTLLDETEEQKRRAAERLHKLRNLSFNVNAADPNNEFETVPAYIRRNMELYNANTQ
                     VENFYSSYEVKTDDKNQTHISSINTFLDGKKPD"
     gene            complement(2816..>3809)
                     /gene="ftsA"
                     /locus_tag="PK977_02535"
     CDS             complement(2816..>3809)
                     /gene="ftsA"
                     /locus_tag="PK977_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRF17009.1"
                     /translation="QIIDVIPQEFTVDNFQNIPNPIGYGGVKIGANFHIITGDKNAIK
                     NINRSVEKAGLYTKDLVLQPLASSSAVMGQEDLEAGVAIVDIGGGTTDLAVFYEGILK
                     HTAVIPFAGENITNDIKTGLGVLKTQAEQMKVQFGSALANEAKANAYITIPGLRGMPA
                     KEISVKNLANIIQARMSEIMDFVTYHLKQVGLDNRMLNGGIILTGGGSQLKHLIQLTE
                     YVTGLPARIGFPNEHWAAGHIEELAKPTYSTCIGLILKGYDDYEHNRKQFEKDFRNVE
                     VPEGLKQAVAEELPAEEETINAKEVVRRKSLTGFWGKFKENIIDLFKEEEDKHL"
     assembly_gap    3810..3860
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3861..4199)
                     /locus_tag="PK977_02540"
     CDS             complement(<3861..4199)
                     /locus_tag="PK977_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRF17010.1"
                     /translation="MNNEQPIIVGLDIGTTKIAVIAGRKNEYGKLEILGFGKANSNGV
                     KHGQVLNIDETIKAIKTALENCFASNPNLTVKEVYVGIAGHHIKSLQTRGDIVRQTTE
                     EEITRKEIDQL"
     gene            complement(4243..5343)
                     /locus_tag="PK977_02545"
     CDS             complement(4243..5343)
                     /locus_tag="PK977_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRF17011.1"
                     /translation="MNAKTTIKKILFITVWLCIGGGMFTLLMAAITKKNRGLCKDYSI
                     SIRGGERNLFIDEKDVEQLLKKATNGSIKGAPVASFNLHDLEHALEDNTWISKAELYF
                     DNQDVLHVTIEEREPVARIFTTTGSSFYIDSMGSSMPLSEKLSARVPVFTGFPAKKRL
                     NSNDSLLLKDVKTIGNYIMKEPFWMSQVAQIDITADRTFEMVPLVGNHLVKLGNGENI
                     AAKFNRLMVFYKQVMSKTGFDKYKLIDVQYKGQVVASRYAGDPKVDSVQLRKNIEKLL
                     RQSVESENDTVLRVLPPVVKLEKDSADTPDPSLQDKPVSNEPKKKDNNDNKSISSQPA
                     KQNKPKPAEPKKVPKAVMPKKEPAEDENGGYN"
     gene            complement(5345..6742)
                     /gene="murC"
                     /locus_tag="PK977_02550"
     CDS             complement(5345..6742)
                     /gene="murC"
                     /locus_tag="PK977_02550"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRF17012.1"
                     /translation="MNTPFRGIDKLNEVYFIGIGGIGMSAIARFFHAGGVKVSGYDKT
                     PTVLTKELEVSGIAVHYEENVELVPKHPDLVVYTPAIPAEHKELEYFREKGAKVVKRS
                     DVLQIITESSFNICIAGTHGKTTITTMVAHLLRDSGFGCNAFLGGISVNYGTNFWSSE
                     RNVCVIEADEYDRSFLKLNPDVAIITAMDADHLDIYGTAEAVEQAFIDFSKRVRPGGL
                     LIRQFGLKRGKELTAGQHIAYSLQNESADVYAANIRMMNGSYEFDVMWKDNMLENVKL
                     NMGGMHNVENAIAAIAVASSLGIENEKIKAAVESFRGVKRRFEYIIKNERLVFIDDYA
                     HHPEELRALITGAKTLFRQKKCTVIFQPHLYSRTRDLADGFAEVLDLADEVILLPIYP
                     ARELPIAGVSSEMILERMKNDNKQVLEKEELMNWIANDYGTTLNKEFGEVLITAGAGD
                     IDVLVEPIMNELKNI"
     gene            complement(6774..>7675)
                     /gene="murG"
                     /locus_tag="PK977_02555"
     CDS             complement(6774..>7675)
                     /gene="murG"
                     /locus_tag="PK977_02555"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRF17013.1"
                     /translation="DIAGFNRSSLIKNIGLPFKLIKSFLQVRKIVKRFQPDAVIGVGG
                     YSSFPVLKTAQAKGIPTFIHESNSFAGKSNMMLGKKATKIFTGTDGMEKFFQKEKIMI
                     TGNPVRTAMVTRTEGLQFFSLEEKKKTVLVVGGSLGAKSINEAIDKGMDDLLNAGLQI
                     IWQTGKPYSAKAKESTSGKQSVWVNDFITQMEYAYAAADIVVARAGAMTVAELCVVKK
                     PVLFVPYPFAAEDHQTVNARQLVNKQAALMVKDDEAMEKLVLMTIELSKDESKQEELK
                     RNIAALAITDADKKIAEEVLKVI"
     assembly_gap    7676..7725
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7726..7861)
                     /locus_tag="PK977_02560"
     CDS             complement(<7726..7861)
                     /locus_tag="PK977_02560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF17014.1"
                     /translation="MSKRVIIAGGGTGGHIFPAIAIANALRKIDGNIKILFIGAKGKM
                     E"
     gene            complement(7867..9132)
                     /locus_tag="PK977_02565"
     CDS             complement(7867..9132)
                     /locus_tag="PK977_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRF17015.1"
                     /translation="MEATRDIGLGGLKNAFNTNNLISKTKGDKVIWALVVLLTLVSLL
                     AVYSATGSLAYKNYRGNTEIYLFKQIAFILVGIMVIYFAHLVNYTFYSKVAKIAFLLS
                     LPLLFYTLFFGVKMNEGSRWIRLPLINMTLQTSDFARLALFMFLARLLSKKQDVIKDF
                     RKGFLPVIIPVAVTCLLIAPANLSTALLLGASCLLLLFIGRVSTKHILITIGVALIPI
                     LFLISAAVIRHGQTKNDEVVVTKKSSSTLFGRVDTWIGRMESFIYGSKDIDDDAYQTT
                     QAKIAIAKGGVFGVGPGNSTTRDYLPQAYNDFIYAIIIEEYGLLGGAFIMFIYLVFLY
                     RCIRIFKKCPFAFGAFLALGLSFTLAIQAVANMAVTVNLFPVTGVTLPLVSMGGTSFI
                     FTCLAIGIILSVARNVEQLEGRTTAEFAK"
     gene            complement(9136..10476)
                     /gene="murD"
                     /locus_tag="PK977_02570"
     CDS             complement(9136..10476)
                     /gene="murD"
                     /locus_tag="PK977_02570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRF17016.1"
                     /translation="MSKRFVILGGGESGVGAALLAKQQGYDVFLSDASSLSNEYRNEL
                     QEAGIAFEEGQHTELQILNADEVMKSPGIPEKAAMVKKIREKGIPVISEIELAYRFKG
                     NSKIVAITGSNGKTTTTALTYHICKEAGLDCALVGNIGDSFAKRVAVDPKPLYVVEVS
                     SFQLDDIKTFRPEIAILTNITEDHLDRYDYKFENYIRSKFRITMNQQKDDVFIYCADD
                     EVTMKYLNQFNIQSNQLPISMKRELPNGAFIKDGDMYVRTGQDFTSMSVYDFALKGKH
                     NQYNTMAACVAATTLDIRKEKIRDAVQNFQSLEHRMEHVATVRGVEFINDSKATNVNS
                     TWYALESMTKPTVLILGGVDKGNDYSLIEELVKEKVKAIICLGTDNRKIHEAFGSTVG
                     TIVNTSTAVEAVHAAFHFAEKGDVVLLSPACASFDLFKNYEDRGSQFKKAVKEL"
     gene            complement(10638..>10840)
                     /locus_tag="PK977_02575"
     CDS             complement(10638..>10840)
                     /locus_tag="PK977_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRF17017.1"
                     /translation="SLSVMMQVGYFKYTKKKYGEGRRVFLKAPLHHHYQVKGYHEVKI
                     VTRFWIVTVLLVVLSIVTLKLR"
     assembly_gap    10841..10951
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10952..11915)
                     /locus_tag="PK977_02580"
     CDS             complement(<10952..11915)
                     /locus_tag="PK977_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRF17018.1"
                     /translation="MLYHLFDWLNSEGIKFPGSRLFDFITFRVLMAVLLSLFITTFYG
                     KKLIKFLQKKQISESIREEGLAGEESKRGTPTMGGIIIILAILIPTILLADLTKVYVQ
                     LMIISTLWMGAIGFIDDYLKLRGKKLAQQRGEQYKKSNKDGLAGWFKILGQVGLGVIV
                     GATLYFNSEVKIWREYVGKVPPTDSTVFSKEVNGKTKYFVEANNSTVTTIPFVKDHEF
                     NYSKLLPASWRGYTWLLYILVVIFIITAVSNGANLTDGLDGLATGTSAIIGTLLGIFA
                     YASGNLVFADYLNIMYIPNLGELSIYIAALIGACIGFLWYNSYPA"
     gene            complement(11926..13389)
                     /locus_tag="PK977_02585"
     CDS             complement(11926..13389)
                     /locus_tag="PK977_02585"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRF17019.1"
                     /translation="MLLSDILYKVNIRSIAGSTAVEVRDVQIDSRKVKKGAAFVAVKG
                     AAADGHQFIDKVIESGALVVVFEEMPALQKEDIVYVQVENSAAAASCMAMNFYGRPAE
                     KLKLVGVTGTNGKTTIATLLYKLFTRLGYTCGLISTVENMIGDQVVPSTHTTPDSISL
                     NQLLSQMVDAGCSHVFMETSSHAIHQHRVTGLQYSGGIFSNITHDHLDYHKTFDEYIR
                     VKKAFFDSLPSSAFAISNADDKRGTVMLQNTNAKKYYYSLKTVADFKGKILDNSLSGL
                     LMTVNEVEVHFRLIGEFNAYNLLAVYGAAICLGEDKQEVLTAMSMLTGAEGRFDYIVS
                     PNEKIIAIVDYAHTPDALLNVLATIKKLKRGFEQVITVVGCGGDRDRTKRPVMAEAAC
                     EHSDKAIFTSDNPRTEDPAQIIKDMEEGLAAAYKRKYISIVDRKEAIKTAISLAKSED
                     IILIAGKGHEKYQEINGVRNHFDDKEVVKEFFELLDK"
     gene            complement(13395..>14321)
                     /locus_tag="PK977_02590"
     CDS             complement(13395..>14321)
                     /locus_tag="PK977_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF17020.1"
                     /translation="PVDLANVPKPTMAPLDQKGSAEGNMLWMSFGYGLQVSPLHTLTL
                     YNAIANNGRMMKPYLVSSIQRNGIITKQFEPTVLEEKLVKPEVVQAARSAMEATVIDG
                     TATRVFKGLPFAVAGKTGTAHVSDGVIKYAHGVYQASFVGYFPADKPQYTCIVVIRTK
                     PHAASHYGGTLAAPVFREIATKLYTMYVQQKNPSMYAAVRDSSLFFYAGNTNDIKNVY
                     RNMNVAFTDSASQHNWANVYSGKNQPVVKPVSVKQQVMPNVRGMGLKDAIHLLENMGL
                     KVAVKGKGKVIMQSVAPGTALAKGITVILELS"
     assembly_gap    14322..14331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14332..15531)
                     /locus_tag="PK977_02595"
     CDS             complement(<14332..15531)
                     /locus_tag="PK977_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF17021.1"
                     /translation="MEVKRDILWRVYLSFLGITILSLFVLGRAFYIQQAEGKHWRSMS
                     DSLHQQFRTLDAERGTIYSEDGQMLSTSIPTFDIYIDFNADGLREKKGKRFHENVDSF
                     AIALADYFADKTAAQYKKELQQAFRKNERYFPLKKKLSFEEYKTFREFPLVRLGRNRS
                     GVIVEVNSKRETPFGLLANRTIGLSREFKDANNKSKKQNVGLERSYDSVLSGKNGQQL
                     VRYIAGGTAIPIEGSQTDPDNGKDIYTTIDVNIQDITETALMRMMLQCEGPYGTAIVM
                     ETSTGKIKAIANLGRRPDGSYWEDDNYALRATEPGSTIKLATLLSVLEKGTSKIDDMV
                     EVGSAGRLQVGNKIVTDAERSPRSVLSVKECFAHSSNVGMSKLAYKASAQKPVELKEF
                     LHRFHLDV"
     gene            complement(15614..15973)
                     /locus_tag="PK977_02600"
     CDS             complement(15614..15973)
                     /locus_tag="PK977_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRF17022.1"
                     /translation="MQEQQNPKERDTQSGPALAGWKRWLNYQSIVKQVPFFLFLAVLA
                     VVYIYNGHYADKTIRKINKTAKEVKELKYEYIAVKSKVMYQSKQSVLVKAAEPLGLKE
                     LTQSPVVLKDSKTNSQN"
     gene            complement(16092..17009)
                     /gene="rsmH"
                     /locus_tag="PK977_02605"
     CDS             complement(16092..17009)
                     /gene="rsmH"
                     /locus_tag="PK977_02605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRF17023.1"
                     /translation="MSEPVYHVPVLLHEVIDGLRINPDGIYVDCTFGGGGHSKAILEK
                     LSPKGKLVGFDQDADAKKNLPDDERLIFVSHNFRHLQRFLKLEGITAVDGIMADLGVS
                     SHQFDEAERGFSTRHNAELDMRMDQRQTLTAFDVVNSYTEQQLHKLFEQYGEVTNAKT
                     LAKTIVQVRSSASLKTIDAFKNALREIVKGNPNKYFAQVFQALRIEVNDELGALKEML
                     QQIPSLLKPGGRVAIITFHSLEDRLVKNFFRRGSFDEPEENPFINTETVNELVIVNKK
                     PILPSERELKQNPRSRSAKLRVAEKVMMI"
     gene            complement(17019..17474)
                     /gene="mraZ"
                     /locus_tag="PK977_02610"
     CDS             complement(17019..17474)
                     /gene="mraZ"
                     /locus_tag="PK977_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRF17024.1"
                     /translation="MTGFLGEFEATLDAKGRFLLPAGFKKQLPEEETPRFVINRGFEK
                     CLALYPVKNWEPLFADISKLNDFDPKVREFRRYFLNGATFVEPDTAGRLLVPPNLKDH
                     AGLQKDIVLVAAVNKIEIWDSNTYKKLFDSYSPDAFSNLAKEVMAKDNN"
     gene            17741..18982
                     /locus_tag="PK977_02615"
     CDS             17741..18982
                     /locus_tag="PK977_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRF17025.1"
                     /translation="MTDPKKISIQDYTYLLPEERIAKYPLAERDASKLLIYREGAIKE
                     DNYKNVAAYIPENSLLIFNNTKVVEARLLFQKPSGGVIEIFCLEPHDQYPDITTAMLQ
                     KEKVWWNCLVGGASKWKPGQLLEKKIKTAEKEIILTAGYIEQKPGSFTIELSWSPASL
                     SFAEILHYFGAIPLPPYIKRAVEEADKERYQTVYAHFEGSVAAPTAGLHFTNNIFSEL
                     KKKNIQRDFVTLHVGAGTFKPVKSDTMAGHEMHAEWIDVSKNTIQNILHNPDKQIVAV
                     GTTSLRTIESLYWLGVLSRVQERESKNTELLLLKQWDAYKFDQYKISTADALSSLLEW
                     MNNKKLERLVTKTQLLIAPGYEFKIAKGLITNFHQPQSTLLLLVAALIGNDWKKVYEY
                     ALKNNFRFLSYGDGSLLWKKD"
     gene            complement(18979..20313)
                     /locus_tag="PK977_02620"
     CDS             complement(18979..20313)
                     /locus_tag="PK977_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF17026.1"
                     /translation="MKRIFTVITVLISLSLVGIIAIQISWLKNMLLLREEQILEKVQE
                     ATKLVGEELGERKGSYAAGGKKSNLFDGLTFDLAKPFTIGKQLTVEEIKDKLRKALAE
                     KGLKDIKFEFGIASFGKSGIENYFERQSENFAAYYENTENKSFVTPLTAPSGSAGENL
                     APNEVLIVVVPNVESQALQSLKWRIIMSGLFTIIIISAFYLTVRTMIRQKKLGEIKND
                     FINNMTHEFKTPIATISLAVDAMRNEKVQQDREKLGYFSGIIKEENQRMNRQVETILK
                     ASQLEKQEVDLNLKPVHVHEVIKDVVDNFALQLDSKNGKVELQLNAQNDLIAADEVHF
                     SNLVNNLVDNAVKYAKDNIPPLIKLSTQSNSKKFIMRIEDNGIGMSRDTVKRIFERFY
                     RAHTGNVHNVKGFGLGLSYVKTMVEAHDGDIKAESILGKGSTFTVELPLKKS"
     gene            20540..>21058
                     /locus_tag="PK977_02625"
     CDS             20540..>21058
                     /locus_tag="PK977_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRF17027.1"
                     /translation="MIEPAPGVLLIADPFLKDPNFLRTVVFLCEHKEEGSFGFVLNRQ
                     YENTLDELIPELEGHKLPVYYGGPVQLDSLHFLHQYPELIPGGQEVIKGVYWGGDFEA
                     LVNLIKNNEIDEKKIRFYIGYSGWSGGQLNEELQEKSWLTAQATRKIVFHKNADDIWK
                     DALKELGGDYEMM"
     assembly_gap    21059..21233
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21343..>21615)
                     /locus_tag="PK977_02630"
     CDS             complement(21343..>21615)
                     /locus_tag="PK977_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P44/Msp2 family outer membrane protein"
                     /protein_id="HRF17028.1"
                     /translation="PQSAVQPYVGLGVGGSLIRYRRFLGEFGESDTKFSFTARPEAGI
                     NIPIGKSRETGINIGAAYHYIPFKHDGVESLSHLGINVGIRFPLRR"
BASE COUNT         5469 a         5056 c         4025 g         6653 t
ORIGIN      
        1 cgctttcagg ataggttcaa taatgtctaa ttgtttaaaa gccataatag gttttatatt
       61 taaatcgcag ttattatctg cggatggagc tggctaagat gttgtagggt ctgaatcagc
      121 gaaagtttgt ctaagtacca ccggcttaca acctgccgtg tcagttatgt ggagtagggt
      181 gcgaagatag ggctttatta tttaacggca tgaagcggtg gctttaccag tttatttctg
      241 tcgttgtata acaatacata ttgttttaaa agaaaatttc tttctgctaa gtttacgttg
      301 ggcaattgct tcaattgttt aaaatgcggc cacccaatat catccaccca gatcaattca
      361 tagtattttg ccggtacaag tatgctgcag atacctaact gcattagatc gttctttnnn
      421 nnnnnnnnnn nncgttcttt tctttttcag acaattcttt ttggagtggc catcctgctt
      481 cctgtctgcc aattaaaata agtatatcat caagggtata ttcttttcca ggctgttttg
      541 tcaatgctga ttgaaattgt aaccagcgct gttgcagttc ttcttcggga agcatggagc
      601 aaaggtagag ctgatatcag atttaaaagg tgagtaaaca aaaccccccg atggaaatcg
      661 gggggaacaa ctaaaaaaca ctagctaaat gctcagtggt ttcattggaa cgaactgcgt
      721 tgaggtttct taagttcacc cttcgacagg ctcagggtga caaaaaacct agtcgggttt
      781 tttcccgtcc aagaaggtat tgatagaact gatgtgtgtt tgatttttat cgtcagtctt
      841 tacttcgtag ctgctgtaga agttttctac ctgtgtgttg gcattatata attccatatt
      901 gcggcggata taagccggca cagtttcaaa ctcattattg gggtcagcag cgttaacatt
      961 gaaagacaag tttctcaact tgtgaagcct ttcagccgcc ctacgttttt gctcttcggt
     1021 ttcgtccaac aaagtcggat cctcaacctc cgcagtcaaa ggtgattgag cctgatgggc
     1081 caacggcata tccgccgcag gaatgtcatc ccgttctact atctgcattt gcaattcgag
     1141 gagttcttcc tcttcttcgc tggcagttgt ttttgcagaa aggaccggtt cttcagcggc
     1201 acgtttcgag gtggtatcct ctgcctgtga tgctgcatag atattggacg gtctggccag
     1261 ataaccccca gatgctgcag acaccggggt cgtactacta gtactatctt tcctgctgac
     1321 tattaactca gcatctgttt cttttatagc ttgttgggga acggatgagt tttgctctgt
     1381 cgaatttata ctatcatctt ttcccactat tgacaatttg ccaacagtag atgtacctac
     1441 cggttcaccc gtagtattac taagtaaaat ttcggtatcg ggcttcagct caaaatgcac
     1501 taccagcggc tcttcttcag ctttgatatc gctgagatca atgacaggcg caggttgcac
     1561 cggtatttct tccaccagct tcggcattaa ataatcttcg gccggcaccg gcttcaatgc
     1621 cgtttccatt acctccattt ctttctcttt catcaatgct tcttctttga tggctactgt
     1681 tttcttttct tcttcatttg tttgcccaag gaccatgacg atcttctctt ctttctttgg
     1741 ttcttccact ttagctaccg gctttgcaaa aggatcttta tgttcaaaac ctgtggcaat
     1801 aagggtgatg ccgagtttat ccccgagatt actgtcgtaa cccataccca gaataacatc
     1861 ggtgttctcc cctgcctggg taagcaggta gttctgtatt acttctactt catccatggt
     1921 aaactcatgt tcaccggtag cagatgtgat attgatgagt atccatttag caccacgtat
     1981 atcattatca ttgagcaatg gtgagttcaa tgcttcttca atggctttct gcgccctgtc
     2041 ttcaccggat gcttctgcat tgcccaaaat tgctacgcca ccactacgca ttacggtaca
     2101 tacatcggca aagtctacat tgatctgccc ggtactgttg ataacatccg tgatacactt
     2161 ggcagctgta gcgagtacat tgtcagcttt ttcaaaagct tctctcattt tcaggttgcc
     2221 aaactggtga cgcagtttgt cgttgctgat caccagcaaa gtgtccacat actgctccag
     2281 gtttttgata ccttccattg cctgctgctg acgtttcttt ccttcgtaag caaaaggtgt
     2341 ggtaacgata cctacggtaa gtatgccgag gtctttacag atcttggcga tgatgggtgc
     2401 accaccggta cctgtaccac cgcccatgcc ggcagtgatg aaggccattt tggtatttac
     2461 ttcgaggatg cgttttattt cttcaagtga ttcttcagta gcctggcggc cgatctccgg
     2521 attggcgccg gcaccgagac cctgtgtaag atggggcccg agctgtatac ggttgggtat
     2581 gccactattg gataatgctt gtgcatcggt attacagatg ataaaattta ctccttctat
     2641 ctgctgactg aacatgtagt tcaccgcatt accgccgccg ccacctacgc cgatcacttt
     2701 taatatggat gatttctctt taggcagatc aaaatgtatc atagcgttga atttagttag
     2761 ccctggtaag gctgttgatt tgggttagta attttcttat gcagttaagg ttcgtttaaa
     2821 gatgtttatc ttcttcttcc ttgaacaggt caatgatgtt ctctttgaac ttgccccaga
     2881 aaccggtcag gctttttctt cttaccactt cttttgcatt gatcgtttct tcttctgcgg
     2941 gcaattcttc ggcaaccgcc tgtttgagtc cctcgggaac ttccacattc ctgaaatctt
     3001 tttcaaactg tttgcggttg tgctcatagt catcataccc ttttagtata agaccgatac
     3061 aggtggagta agtaggcttg gccagttctt caatatgacc agccgcccaa tgttcattgg
     3121 ggaaaccaat tcttgcagga aggccggtta catattctgt taactgtatc aaatgtttta
     3181 actgggaacc accaccggta agaatgatac caccattcag catacggtta tccaaaccta
     3241 cctgcttgag gtgataggta acaaaatcca tgatctcact cattcttgcc tggatgatat
     3301 tggccaggtt ctttacgctg atctccttgg caggcattcc ccttaacccg ggaatagtga
     3361 tataagcatt ggcttttgct tcattagcca gggcactgcc gaactgtact ttcatttgct
     3421 cggcctgtgt cttcaatacg cctaaaccag ttttgatatc gttagtaata ttttcaccgg
     3481 caaaaggaat aacagccgta tgttttaata tgccttcgta gaatacagca aggtcggtag
     3541 taccaccacc aatatccacg atggccacac cagcttccag gtcctcctgt cccattacag
     3601 ctgaagaaga agccagcggt tgcaacacca ggtctttggt gtacaaacct gctttctcca
     3661 cactgcggtt gatgttcttg atcgcattct tatcgccggt gatgatatga aagttggcgc
     3721 caatttttac tccgccatac ccgatggggt tgggtatatt ctgaaaatta tctactgtaa
     3781 attcttgcgg gataacatca atgatctggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn cagctgatca atctctttgc gggtaatttc ttcttccgtt
     3901 gtctggcgta caatatcgcc acgggtctgc aagcttttga tgtgatggcc ggcaataccg
     3961 acatacactt ctttcacagt caggtttggg ttggacgcaa agcaattctc cagcgccgtt
     4021 ttgatcgcct tgatggtctc atcgatgttg agcacctgtc cgtgcttcac accgttggaa
     4081 ttggcttttc cgaagccaag tatctccagc ttaccgtatt cgttcttacg accggcaatt
     4141 actgcgatct tggtagttcc aatgtccaga ccgacgataa tgggttgttc gttgttcatg
     4201 atagttgttt ttagttgttg ttttatagtc gaaatagttg ttttagttat agcctccgtt
     4261 ctcatcctct gcaggctcct tcttcggcat cactgccttc ggcactttct ttggctcagc
     4321 cggcttgggc ttattctgtt tagcgggttg ggatgaaata gatttgttat cgttattatc
     4381 ttttttcttt ggttcattgc ttacaggttt gtcctgcaaa gaaggatccg gtgtatctgc
     4441 agaatctttt tccagtttta ctaccggtgg taatactctt agtactgtat cattttctga
     4501 ttcaacagac tggcgaagca gcttctctat attctttctt aactgcacag aatccacttt
     4561 tggatcaccg gcatagcggg aagccactac ctgtccttta tactgcacat caatcaattt
     4621 atacttatca aatcctgttt tgctcattac ctgcttatag aaaaccatca ggcggttgaa
     4681 tttggcagct atgttctctc cattgcccag tttcaccagg tgattaccca ctagtggtac
     4741 catttcaaat gttctgtctg ctgttatatc aatctgtgcc acctgcgaca tccagaatgg
     4801 ttccttcatg atatagttac caatcgtttt cacatctttt agtaaaagac tatcattact
     4861 gttcagcctt ttcttagccg ggaaccctgt aaataccggc acccttgcac tcaacttctc
     4921 tgacaatggc atgctgcttc ccatactatc aatatagaat gagctgccag ttgtggtaaa
     4981 tatccttgcc actggttctc tttcttcaat cgttacatgc aacacatcct ggttatcgaa
     5041 atacagttct gccttgctga tccaggtatt atcttccaaa gcatgttcca ggtcatgaag
     5101 attgaatgat gcgaccggtg ctccttttat ggacccattg gtggctttct tcagcaactg
     5161 ctccacatct ttttcatcaa tgaacaaatt cctctctcct ccccgtatac taatgctgta
     5221 atccttgcac agtcccctgt tctttttggt aatagctgcc atcagcaggg taaacatacc
     5281 tccgcctata cacagccata ctgtgatgaa taaaattttc tttattgttg ttttagcgtt
     5341 cactctaaat attctttaac tcattcatta tcggttctac cagtacatct atatcgcctg
     5401 cgccggctgt tatcagtact tctccaaatt ccttattcag cgtagtacca tagtcattag
     5461 ctatccagtt catcagctct tctttttcca acacctgctt attatcgttc ttcattcttt
     5521 ccagtatcat ttcactgctc acacctgcta ttggcaattc ccttgcagga tagatgggca
     5581 gcaaaatcac ttcatctgca aggtccagca cttctgcaaa gccatcggca aggtctcttg
     5641 tgcggctgta caggtgtggc tgaaatatga ctgtacattt tttctgcctg aacaaagtct
     5701 ttgccccggt gatcaaagcc ctgagttctt ccggatggtg agcataatcg tctataaata
     5761 ccagtcgttc atttttgatg atgtactcaa accttctctt caccccgcgg aaactctcca
     5821 ctgctgcttt gatcttttca ttttcgattc ccaaagaact ggccactgct atagcggcaa
     5881 ttgcattctc tacattatgc attcctccca tgttcagctt cacattctcc agcatattat
     5941 ctttccacat cacatcaaac tcataactgc cattcatcat ccggatattg gctgcatata
     6001 catcagcact ttcgttctgc aggctgtatg ctatatgctg accggccgtt aactcctttc
     6061 ccctttttaa tccaaactgc ctgatcaata atccgcccgg tcttactctt ttactgaagt
     6121 caataaaagc ctgttccact gcttcagccg taccatatat atccaggtgg tcagcatcca
     6181 tggctgtgat gatggccaca tccgggttca gtttcaaaaa acttctgtca tactcatctg
     6241 cctcaataac acatacattt ctttcactac tccagaaatt ggtaccgtag ttcaccgaaa
     6301 taccaccgag aaatgcatta cacccaaaac cactgtcgcg gagcaaatgc gccaccatcg
     6361 tggtaatcgt tgttttgcca tgtgtaccgg caatacatat attaaaagaa ctctctgtta
     6421 ttatctgtaa tacatcactt ctcttcacca ctttagcacc tttctccctg aagtactcca
     6481 gctctttatg ttctgccggt atagctggtg tatacactac caggtcaggg tgctttggaa
     6541 ccaattccac attttcttca tagtgcactg ctataccact tacctccagt tcttttgtca
     6601 gtacggttgg cgtcttgtca tacccactta cttttacacc cccggcatga aagaaccttg
     6661 caatagcact catcccgatg ccaccaatac cgatgaagta cacttcattc agtttgtcaa
     6721 tcccccggaa gggggtattc ataaggacag tattgacttg tacgttcatg cggctaaatc
     6781 actttaagca cttcttccgc tattttttta tcagcatcgg ttatggccag tgctgctata
     6841 ttccttttca attcttcctg cttgctttca tctttcgaaa gctcaatggt cattaaaacc
     6901 agcttttcca ttgcttcatc atctttcacc atcaatgctg cctgcttatt caccagttgc
     6961 cgtgcattca ccgtctggtg atcttcagca gcaaaaggat agggtacgaa caaaacgggc
     7021 tttttaacaa cacataactc agctacggtc atcgctcctg cacgggccac cactatatcg
     7081 gcagcggcat aagcgtattc catctgtgtg atgaaatcgt tgacccaaac tgattgtttt
     7141 ccactggtac tttcctttgc ttttgctgaa taaggtttac cggtctgcca gatgatctgc
     7201 aaacctgcgt tgagcaaatc atccatgcct ttatcaatgg cttcgttgat ggatttggcc
     7261 ccaaggctgc cacccaccac caggacagtt ttcttttttt cttccaatga gaaaaactgc
     7321 aacccctcag ttcttgtcac cattgctgta cgcaccgggt tgccggtgat catgatcttt
     7381 tccttttgaa aaaatttttc cattccatct gtaccggtaa aaatttttgt tgcctttttt
     7441 cccagcatca tatttgattt tccggcaaat gaattggact catgaatgaa agtggggatt
     7501 ccttttgcct gcgctgtttt caacacagga aaactggaat atcctcccac cccgatcact
     7561 gcatccggct gaaaacgttt tacaattttt cttacttgta aaaaactttt gatcagcttg
     7621 aatggtaatc caatgttctt tatcaaagag ctgcgattga accccgctat atctannnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttcc atttttcctt
     7741 ttgcccctat aaagagtatt ttaatattcc cgtcaatctt tctcagtgca ttggcaattg
     7801 caattgccgg aaaaatatgt ccgcctgttc ctcctcccgc tatgatcact ctcttgctca
     7861 tttcactcat ttagcaaatt ctgctgttgt ccttccttcc agttgctcta cgttccttgc
     7921 cacactcaat atgataccaa tagcaagaca ggtaaaaatg aaactggttc ctcccatact
     7981 aaccagcggc agcgttactc ccgttacagg aaataagttt accgttaccg ccatatttgc
     8041 tactgcctgg atggccagtg taaaacttag tcccaatgcc agaaaagcac caaaagcaaa
     8101 gggacatttc ttgaaaatcc tgatacaccg gtacaggaat accaggtaaa taaacatgat
     8161 gaatgcaccg cctaacaaac catattcttc tatgataatg gcataaataa aatcattgta
     8221 tgcctgtggc aaataatctc tagttgtact gttaccgggg cctactccaa atactccgcc
     8281 tttagctata gcaatcttgg cctgcgttgt ctggtaggca tcatcatcaa tatccttgct
     8341 gccataaata aaactttcca tcctgcctat ccaggtatcc acccttccga ataaggtgga
     8401 agatgatttc tttgtcacaa ctacctcatc attctttgtt tgcccatgtc ggatcaccgc
     8461 tgctgaaata agaaagagga ttggtatcaa tgctacacca atggttatca aaatatgttt
     8521 ggtactaacc ctgccaatga acaagagtaa aagacaactt gctcccagta ataaagctgt
     8581 ggacaggttg gcaggcgcaa tcaacagaca ggtaacggca actggtataa tcaccggtaa
     8641 aaaacctttc ctgaaatctt tgatcacatc ctgcttcttg cttaacaggc gggcaaggaa
     8701 catgaacaag gccagtcttg caaagtcgga agtctgcaac gtcatattaa taagcggcaa
     8761 acggatccag cggctaccct cattcatctt tacaccaaag aataatgtat agaataataa
     8821 aggcagagaa agcagaaaag caatcttcgc cacttttgaa tagaacgtat aattaacgag
     8881 gtgagcaaag taaataacca ttattcccac caggataaat gcaatttgtt taaagaggta
     8941 gatctctgta ttaccccgat agttcttata tgctaatgac ccggtagcac tgtaaacagc
     9001 cagcagggaa actaatgtca gcaataccac caacgcccaa atcaccttat cacctttagt
     9061 cttactgata agattattgg tattgaaggc attctttaac ccgcccaatc ctatatctct
     9121 tgttgcttcc attaattata gttctttcac tgctttttta aactggcttc ccctgtcttc
     9181 ataatttttg aacaaatcaa aactggcgca ggcagggctc agcaatacta catctccctt
     9241 ctccgcaaaa tgaaaggcag catgcactgc ttccaccgca gttgatgtat ttacaatggt
     9301 tcctactgta ctaccaaaag cctcatgtat ctttctgtta tctgttccca ggcaaataat
     9361 tgcttttact ttttctttta ccagttcctc tattaatgag taatcgtttc ctttatctac
     9421 accacctaat atcaatacgg tcggctttgt catactttcc agcgcatacc aggttgagtt
     9481 gacattggtc gccttactgt cattgataaa ttccactcct cttactgtag ccacatgttc
     9541 catcctgtgc tccaggcttt ggaaattttg taccgcatca cggatttttt ctttccttat
     9601 atccagtgtg gtggcggcta cacaagctgc catagtatta tattggttat gttttccttt
     9661 caaagcgaaa tcataaacac tcattgaggt aaaatcctgt cctgttctta catacatatc
     9721 cccgtcttta ataaatgctc cgtttggtaa ttctcttttc atactaattg gcagttggtt
     9781 tgattgaata ttgaactggt ttaaatattt catagttact tcatcgtcgg cgcagtagat
     9841 gaatacatca tctttctgct ggttcatagt gatacggaat ttgctcctga tataattttc
     9901 gaatttgtaa tcgtatcggt ccaggtgatc ttcggtgata ttggtcagta tggcgatctc
     9961 aggtctgaat gtctttatgt catccagctg aaagctgctt acttctacta cgtacaatgg
    10021 ctttggatca acagctaccc ttttggcaaa tgaatcaccg atattgccca ccagtgcaca
    10081 atccaggccg gcctccttgc agatatggta ggtcagcgct gtggttgtgg tctttccgtt
    10141 actgccggtg atggcaacga tcttgctatt gcctttaaac cggtaagcca gctctatctc
    10201 actgataacc ggaatacctt tttccctgat ctttttaacc atcgctgctt tttcaggaat
    10261 gccggggctc ttcatcactt catctgcatt tagtatttgc aactccgtgt gttggccctc
    10321 ttcaaaagcg attcccgctt cctgtaactc gttacgatac tcattactca aagaacttgc
    10381 gtcggaaagg aaaacatcat acccctgttg cttcgccagc agcgcagcac ctacgccgct
    10441 ttcgccacct cccagtatga caaacctttt actcatcatc tgtttcgata agtgtacgga
    10501 attgggttct tcataagtat tagttttcaa tacaactgca ccacaattcg attggatcag
    10561 aacatcatca gtcttctaca gggtttcggt acggggtttt tcagtaatat cttttcaata
    10621 acagttaacc gtcgtattca tcttaatttc aatgtcacaa tactcaacac taccagcagc
    10681 actgtcacaa tccagaacct tgttacaatt tttacttcat gatatccctt aacctggtag
    10741 tgatggtgca atggtgcctt caaaaacact cttctccctt ctccatattt tttcttcgta
    10801 tacttgaaat accccacctg catcatcaca ctaagcgact nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgcgggat acgaattgta ccaaaggaaa
    10981 cctatacatg ccccaatcaa tgctgcgatg taaatggaca actcaccaag attgggtatg
    11041 tacatgatat tgagataatc ggcaaacacc aggttaccgc tggcataggc aaatatgccc
    11101 agcagtgttc cgatgatagc agaggtgcct gtagcaaggc catctaatcc gtccgtaaga
    11161 ttggccccgt ttgatacagc agtgataata aatatgacaa ccagtatgta gagcagccat
    11221 gtgtaccctc tccaggatgc cggcagcagc ttggagtaat tgaactcatg gtcttttaca
    11281 aacgggattg ttgtcacagt tgaattattt gcctcaacga aatattttgt tttgccattc
    11341 acttctttgc taaacaccgt tgaatctgtt ggcgggacct tacccacata ctctctccat
    11401 atctttactt cgctattgaa ataaagggta gcaccaacaa ttacacccaa acccacctgt
    11461 ccaagtatct tgaaccagcc ggctaatcca tctttatttg acttcttata ctgctctcct
    11521 ctttgctgtg ccagtttctt acctcttagc ttcaggtagt catctataaa ccctatggcc
    11581 cccatccaca gtgtactgat gatcatcagc tgcacataaa cctttgtcag gtcggccagc
    11641 agaatggtgg gtatcagtat cgccaggatg atgataatac cgcccatagt cggcgttcct
    11701 cttttgctct cttcgccggc aaggccctct tccctgatac tttcacttat ctgctttttc
    11761 tgtaaaaact ttatcagctt cttaccgtaa aaagtggtga tgaataaaga aagtaatacc
    11821 gccatcagca cccggaaggt gatgaagtcg aagagcctgc ttcccgggaa ctttatccct
    11881 tcactgttca gccagtcaaa taagtgatac agcatgctgg tttggttact tatctaataa
    11941 ttcaaaaaat tctttaacta cttctttatc atcaaagtga tttctcactc cgtttatctc
    12001 ctggtacttc tcatgtcctt tgccggcaat caggataata tcttctgact ttgccaggct
    12061 aatcgccgtt ttgatcgctt ccttcctgtc aacaattgag atatactttc ttttatacgc
    12121 cgctgctaat ccttcttcca tatcctttat aatctgcgcc gggtcttctg ttcgtggatt
    12181 gtcactggta aaaatggctt tgtcactatg ctcacaagca gcttcagcca tcacgggtct
    12241 tttcgttctg tccctgtcac caccgcagcc aactactgtt atcacctgct caaaccctct
    12301 tttcaatttt ttgatcgttg ccagcacatt cagcaaagca tccggggtat gtgcataatc
    12361 aacaatggca attatttttt cgttaggcga aacgatataa tcaaaccttc cttcagcacc
    12421 ggtcagcata ctcatcgctg tcaatacttc ttgtttatcc tcacccaaac agatggctgc
    12481 accataaaca gccagcaggt tgtaggcatt gaattccccg atcagtctga aatgcacttc
    12541 cacttcattc actgtcatca gcaaaccact caggctgtta tccaggatct ttcctttgaa
    12601 atcagccact gtcttcaggc tgtagtaata cttttttgcg ttggtattct gcagcattac
    12661 tgttccccgc ttatcatcag cattactgat ggcaaaggca gaagagggca atgaatcaaa
    12721 gaaggccttt ttcacccgga tatattcatc aaacgttttg tgataatcca ggtgatcatg
    12781 ggtgatattg ctgaaaatac cacccgaata ctgcaaacca gttaccctgt gctgatgaat
    12841 agcatgagaa ctggtttcca tgaacacatg actgcaacct gcatccacca tctgactcag
    12901 taattggttc aggctgatgg aatcgggtgt agtatgcgta gaaggcacca cctggtcacc
    12961 aatcatattc tccaccgtgc tgatcaaacc acatgtatat cccaatcttg taaagagctt
    13021 atataataat gttgcaatag ttgtttttcc gtttgttcct gttaccccaa ccagttttag
    13081 tttctctgct ggcctgccat aaaaattcat cgccatacag gaagctgctg cagcgctatt
    13141 ctcaacctgc acatacacta tatcttcctt ctgcagagcc ggcatttctt caaaaaccac
    13201 taccaatgcc ccactttcta ttactttatc aatgaactga tgaccatctg ctgctgctcc
    13261 tttcactgca acgaaggctg ctcccttttt cacttttctc gaatcgatct gtacatctct
    13321 cacctccacc gctgtacttc ctgctatcga cctgatgtta accttatata gtatatcact
    13381 taacagcact tgctttaact taactccagt attacagtaa ttcctttagc caatgccgtt
    13441 cccggcgcca cggattgcat gatcactttt ccctttcctt tcactgccac tttcagtccc
    13501 atattttcca gaaggtgaat agcgtctttt aaacccatac ctcttacatt gggcatcact
    13561 tgttgcttta cagaaaccgg ttttactacc ggttgatttt ttcctgaata aacattagcc
    13621 cagttatgtt gcgatgctga atctgtaaaa gcaacattca tattcctgta gacattcttt
    13681 atatcatttg tattacctgc ataaaagaac agggaactgt ctctgacagc ggcatacata
    13741 gaagggttct tctgctgaac atacattgta taaagcttgg tagcaatctc cctgaaaaca
    13801 ggagcggcta atgtgccgcc atagtgtgaa gctgcatggg gtttggtacg aataacaaca
    13861 atacaagtgt attgcggctt atctgccgga aagtagccaa caaatgaagc ctggtaaaca
    13921 ccatgtgcat atttaattac cccatcggaa acatgtgctg taccagtttt acctgctacg
    13981 gcaaatggca gtcctttaaa aacccttgtt gccgttccat caattacagt tgcttccatt
    14041 gcggatcttg ctgcctgcac aacctcaggt ttaactagct tttcttccag cacagtgggt
    14101 tcaaattgtt tggtgataat tccattacgc tgaatactgc ttaccaggta aggtttcatc
    14161 atccggccat tattagcaat ggcattatat aatgtcagtg tatgtaaggg gcttacctgc
    14221 agaccataac caaaactcat ccaaagcata ttaccttccg cacttccttt ctgatcaagc
    14281 ggcgccatcg ttggcttggg tacattagcc aggtcaactg gnnnnnnnnn ntacatccag
    14341 gtgaaagcgg tgcaggaatt cttttaactc aaccggtttt tgtgcagagg ctttataagc
    14401 cagtttactc atacccacgt tagaactgtg ggcaaaacat tctttaactg acaacacaga
    14461 acggggggat ctttcagcat ctgtaacaat tttgtttccg acctgcaatc ttccggcact
    14521 acctacctct accatatcat caatcttact ggtgcctttt tccagaacgg ataaaagtgt
    14581 ggccagctta atagtagaac ctggctctgt agctctcaaa gcataattat catcctccca
    14641 ataactgcca tcaggccggc ggccaaggtt agctattgct ttaatcttgc cggtacttgt
    14701 ttccattaca atagcagtac catatggacc ttcgcactgc aacatcatcc gcatcagggc
    14761 cgtttcagtt atgtcctgga tgtttacatc aatggttgtg taaatgtctt tcccgttatc
    14821 cggatccgtt tgagagcctt cgatcgggat ggctgttccg cctgcgatat aacgaaccag
    14881 ttgctggcca ttcttaccgc tcaatacaga atcataactt ctctcaagtc ctacattctg
    14941 cttcttggat ttattattag catctttaaa ctctctggat aaaccgatcg ttctgttggc
    15001 caataatcca aagggtgttt ctcttttact gttcacttca acgatcacac cacttctgtt
    15061 gcggcccaat cttacaagtg gaaattcacg aaaagtttta tactcttcaa aactcagttt
    15121 cttttttagg gggaagtatc tttcattctt tctgaaagcc tgttgaagct cttttttata
    15181 ttgtgctgct gttttatcgg caaaataatc cgccagggct atggcaaaag aatcaacatt
    15241 ctcatgaaaa cgttttcctt tcttttccct taaaccgtct gcattaaaat caatgtaaat
    15301 atcaaaagtg ggaatggaag tgctgagcat ctgaccgtcc tcactgtaga tagtacctct
    15361 ttctgcatca agtgtccgga actgttggtg aagactgtca ctcatactcc gccagtgctt
    15421 gccttcagcc tgctgaatgt agaaagccct gccgagtaca aataaactta gtatggttat
    15481 accaagaaaa ctgagatata ctctccaaag tatgtcacgt tttacttcca aggcccttct
    15541 gcttgtgttc gttagttgtt cgttgtatcc gtccaccttt ccggttcagg gaaaggattt
    15601 tagttgttcg ttcctaattc tggctgttcg tcttactgtc tttcaagacc acaggtgact
    15661 gagtcagctc ctttaatccc agtggttccg ccgcttttac caatacactt tgcttactct
    15721 ggtacatcac cttacttttc actgctatat attcgtactt cagttctttt acttctttgg
    15781 cagtcttgtt gatcttacga atggtcttat ctgcatagtg accattgtag atatacacta
    15841 ccgccagcac tgcgaggaac aaaaagaacg gcacctgttt tacgatcgac tgataattca
    15901 gccaacgctt ccatcctgct aaggcagggc cggattgtgt gtcccgttct tttggatttt
    15961 gctgttcctg catacatgag ttgttagctc gttagtttgg tttttcatct tctcttttga
    16021 gaagggttga tttcaaagga tatagatttt ccagattttt atacccctat atttactgta
    16081 tgaactctta tttatatcat catcactttt tccgccactc tcagctttgc acttcttgac
    16141 cttgggtttt gtttcaattc tctttccgaa ggaagtattg gtttcttgtt tacgataacc
    16201 agctcattca ctgtttctgt attgataaag gggttctctt ccggttcatc gaatgaacca
    16261 cgacgaaaga agttctttac cagcctgtcc tcgagggaat gaaacgtaat gatggcaaca
    16321 cggccgcctg gttttaacag ggatggaatc tgctgcagca tctctttcaa tgcacctaat
    16381 tcatcgttca cttcaatgcg caaagcctgg aaaacctggg cgaagtactt attcggattt
    16441 ccttttacta tttcccggag tgcattctta aacgcatcta tcgtttttag cgatgcagag
    16501 cttctaacct gaacaattgt tttggctaaa gttttggcgt tggtcacttc cccatactgt
    16561 tcaaaaagtt tgtgcaattg ttgttcagta taggagttca ccacatcaaa tgcggtcagt
    16621 gtctgccgct gatccatccg catatccagt tcagcgttgt ggcgggtgga gaaacctctc
    16681 tcagcttcgt caaactggtg actgcttacg ccaagatcgg ccataatacc atcaacagcc
    16741 gttatcccct ctagtttcag aaaacgctgc aggtgccgaa aattgtgtga aacaaaaatt
    16801 aatctttcgt cgtccggcag gtttttcttt gcgtcggcat cctggtcaaa accgaccagt
    16861 ttgccttttg gacttagctt ttcaagaatg gcctttgagt gaccaccccc tccaaatgtg
    16921 caatccacat aaattccatc ggggttgatg cgaagaccat cgatgacttc gtgtaacaaa
    16981 accggtacat ggtaaacagg ttcactcatg agtatcggtt aattattatc cttcgccatc
    17041 acttccttcg ccaggttgct gaatgcatcc ggtgaatatg agtcaaagag ctttttgtac
    17101 gtattactat cccagatttc aattttatta accgccgcca ccagcactat atctttttgc
    17161 aaaccagcat ggtctttcag gtttggcggc accagcaacc tgcccgctgt gtccggctcc
    17221 acgaaagtgg cgccattcaa aaagtagcgg cgaaactccc tcaccttagg gtcgaaatcg
    17281 ttcagtttac tgatgtctgc aaagagcggt tcccagtttt ttaccgggta aagagctaaa
    17341 catttttcaa aacccctgtt gataacgaaa cgaggtgttt cctcttccgg caattgcttt
    17401 ttgaaccccg ccgggaggag gaaccgcccc ttggcgtcca aagtagcctc gaattctccg
    17461 agaaagcctg tcattatcag tgaattgggt caattttgaa ttaattgaca caaaataaca
    17521 ctttttccca cttaaatacc aaatttttcc atttttcttt tttccaccat caaaattttc
    17581 ccgaaagcca atacagtaaa gggtttcagc cggtgtggga aaaatttgaa gcaaattgag
    17641 gtgaatagac tgtgaattgc ggtggaaaag ctgtggattg tgtaattaag gaggcaattc
    17701 agatatttgc acctcaccaa atacaacagt cagttgtgtt atgacagatc caaagaaaat
    17761 atccatacag gattatacct acctgcttcc cgaagaacgg attgccaaat atccactggc
    17821 agaaagagat gcttccaaac ttcttattta tagagaagga gcgattaaag aggataacta
    17881 taaaaatgta gccgcataca ttcctgaaaa ttccttactt atttttaata ataccaaagt
    17941 ggtagaagca agattgcttt tccaaaaacc ttccggaggt gttattgaaa tattttgtct
    18001 ggaaccccat gaccaatatc ctgatatcac tactgctatg ctgcaaaaag aaaaagtatg
    18061 gtggaattgt ttagtaggcg gagcttccaa atggaaaccg ggccaattgc ttgaaaagaa
    18121 aataaagaca gcagaaaaag aaattatcct tacagcgggg tacattgagc agaaacccgg
    18181 ttcgtttacc attgagctct catggtcgcc tgcttctcta agctttgctg aaatacttca
    18241 ctacttcggc gctattcctt tgccccctta catcaaaagg gctgtcgaag aggcggataa
    18301 agaaagatac cagactgtct atgctcattt cgaaggatca gtggctgcac ccacagccgg
    18361 acttcatttc acaaacaata tatttagtga attgaagaaa aaaaatattc aaagagattt
    18421 tgtaacgctg catgtgggtg ccggaacatt taaaccggta aaaagtgata ccatggctgg
    18481 acatgaaatg catgcagaat ggattgatgt ttcaaaaaac accattcaaa atatcctcca
    18541 taacccggat aaacaaattg tagctgttgg aaccacttcg ttgagaacaa tagaaagttt
    18601 gtattggctc ggcgtattga gtcgtgtaca ggaaagagaa agcaaaaaca cagaattgct
    18661 tctgcttaaa caatgggatg cttataaatt tgatcagtat aagataagta ctgcggatgc
    18721 tttatcatcc ctcctggaat ggatgaacaa taaaaaactg gaaagactgg ttaccaaaac
    18781 acaattattg attgcacctg gttatgaatt caaaattgca aaaggtctta tcaccaattt
    18841 tcatcaacca cagtctaccc ttctcctcct cgtcgctgcg ttaattggca atgattggaa
    18901 gaaagtatac gaatacgcat tgaaaaataa tttccggttt ctgagttatg gtgacggaag
    18961 tctgttgtgg aaaaaggatt aagatttctt caaagggagt tcaactgtaa aagtactgcc
    19021 cttacccagg atgctctcag cttttatatc cccatcgtga gcttctacca ttgtctttac
    19081 ataacttaag cccaatccaa aaccttttac attgtgtaca ttaccggtat gggccctgta
    19141 gaaacgttcg aagatccttt tcactgtatc cctgctcata ccaataccgt tgtcttcgat
    19201 ccgcatgata aatttcttgc tgttggactg tgtagacaat ttgatcaatg gaggtatatt
    19261 gtcttttgca tacttaacgg cattatcaac caggttatta accaggtttg aaaagtgtac
    19321 ttcgtctgcg gcaataaggt cattctgtgc attcagctgc aactctactt ttccattttt
    19381 ggaatcaagc tgtaaagcaa aattgtccac aacatctttt atcacttcgt gtacatgaac
    19441 cggcttcagg ttcaagtcaa cttcctgttt ttccagttga gaggccttta aaattgtttc
    19501 cacctgccgg ttcatccgct ggttctcttc ctttatgatt ccgctgaaat aaccaagttt
    19561 ttccctgtcc tgctgcacct tttcatttcg catggcatcc accgccaatg aaatagtggc
    19621 aatcggtgtc ttgaattcat gcgtcatgtt attaataaag tcatttttaa tttcacccag
    19681 tttcttttgc ctgatcattg ttctgacagt gagatagaaa gcagaaataa taatgatggt
    19741 gaataatcct gacatgatga tcctccattt taaggattgc aatgcctggc tctcaacatt
    19801 aggaacaaca acaatcagca cttcattggg tgcaagattt tcaccggcac tgccgctggg
    19861 tgctgttaaa ggagtaacaa aggatttgtt ctctgtattc tcataatagg cagcaaaatt
    19921 ctcagactgc cgttcaaaat aattttctat gccgcttttg ccaaatgagg cgatgccaaa
    19981 ttcgaatttg atatccttca agcccttttc agccaaagcc tttcttaatt tatcttttat
    20041 ctcttcaaca gttagttgtt ttccgattgt aaaaggtttg gcaaggtcaa atgtcaggcc
    20101 atcaaaaaga tttgattttt tcccgcctgc ggcataactg ccctttcttt cacccagctc
    20161 ctcgcctacc agtttggtag cctcctgtac tttctccagg atctgttcct ctctcagcaa
    20221 cagcatattt tttagccatg aaatctggat ggctataatg ccaaccagtg aaaggctaat
    20281 caaaactgtt atgacggtaa aaattcgttt cacaaaaaaa atctacaaac ctgaaagata
    20341 aatggttttc ttaaaacaga ttaatgttgc aaaaatacat ttccattacc ggtagccatc
    20401 ccctgcatgg ggtatatggc tgattttaac ggtgttttac caatcacatt ataatatacc
    20461 ggcaggttgc ttttattggc acttatcccc ttttttaaac agcttttttg caaaatgatt
    20521 ttatatacta aattgaagta tgattgaacc tgcacccggc gtattactga tagcagatcc
    20581 ctttctgaaa gaccccaact ttcttcgcac cgtggttttt ctatgcgaac ataaagagga
    20641 aggaagcttt ggctttgtat tgaaccgtca atatgaaaac acattagatg aactgatacc
    20701 ggaactggaa gggcataaac taccagtgta ttatggagga ccggtacaat tagattccct
    20761 gcattttcta catcaatacc ccgagctcat tcccggcgga caagaagtga tcaaaggtgt
    20821 atactggggc ggcgattttg aagcccttgt caacctgatc aagaataatg agattgatga
    20881 aaagaagatc cgtttttata tcggttattc gggatggtcc ggcggacaac tgaatgaaga
    20941 attacaggag aaatcgtggc ttacggcgca ggctacgcga aaaatagtat ttcataaaaa
    21001 tgccgacgat atctggaaag acgcgttaaa ggaacttggc ggcgattatg aaatgatgnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttcaaa
    21241 acgacgcgac taatgcaagg gtccgactgg cttgtggacg cgtctgccgc ggtccgttca
    21301 tgtattatct acgtatatag cgaacagtta tatcatctgc agctacctgc gcaagggaaa
    21361 tcttatccct acattaatgc ccaaatgact caagctctct acgccatcgt gtttaaaagg
    21421 aatgtaatga taggccgcgc caatattaat tcccgtttcg cggcttttac caataggaat
    21481 attgattccc gcttctgggc gagccgtaaa actgaacttg gtatcgcttt cgccaaattc
    21541 tccaagaaat ctgcggtacc gtatcaggct tcctccaact cctaagccca cataaggctg
    21601 aacagccgac tgcgg
//
LOCUS       DAORLS010000016        20566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1400_length_20566_cov_2.511384, whole genome shotgun sequence.
ACCESSION   DAORLS010000016 DAORLS010000000
VERSION     DAORLS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_20566_cov_2.511384"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..309
                     /locus_tag="PK977_02635"
     CDS             178..309
                     /locus_tag="PK977_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17029.1"
                     /translation="MKHVKEYQRGEGGIVIMSNDAHIEVSRRKKEQFLEKIKEIYKY"
     gene            complement(383..979)
                     /locus_tag="PK977_02640"
     CDS             complement(383..979)
                     /locus_tag="PK977_02640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF17030.1"
                     /translation="MKFLLLVFILLMVGCSVSKNPLRKQVKLLQKGILKDDTSYVYAL
                     PFPERKKYRVIQGYFSRFTHKERAALDFNMKKGDTITAARDGIVVRAKEDGKKGGLNR
                     KYRSEGNNIVIQHADGSRAGYWHLQHNGVLVNIGDTVKKGQPIALSGKTGYAFVPHLH
                     FLVWRSGGGQWQQVPTRFQTSKGIMYLRSWRKYKKPAQ"
     gene            1214..>2168
                     /locus_tag="PK977_02645"
     CDS             1214..>2168
                     /locus_tag="PK977_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF17031.1"
                     /translation="MSSRDVKDACKDKDGFIWFGTSNGLNRYDGSGFKIYSRQYKNSR
                     SLTSDIINCVSEVDGNIWIGTPTGLFVLDKKMDTIRPVLFTPNRSTGVLKFEKDFENK
                     TWIITDHRIFILNKGIPEPIEKTYPGTARLVTYKTFNTVERDSFRKGIWFSADNNLFF
                     FHQQSGLFFSQENNPYNWSIFQTGHAISLAVDATGKIWFITSLLPYLQLFDFTTNTSS
                     RIMDSGFEYPKVLKSVNAVGKLMTDSKGRIWISTWGGELLMNIGPNKIINVSEQSITG
                     YKPAFTVFETIYSDDAGNDWLLGKDGANKIWGNSVFDYVTQL"
     assembly_gap    2169..2325
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2326..4735
                     /locus_tag="PK977_02650"
     CDS             <2326..4735
                     /locus_tag="PK977_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF17032.1"
                     /translation="SPDNYRANRFFHAIFYRNEWWFATGDGILILNPVSNRFRKFTHY
                     AKGYEIKNKSVTNLLVDKKGKLWFSVWNDALYRYDPVTSQTVRYDGHDAGQGDIRTLN
                     CSSASEDIEGNIWITSQLLRKFEITKEKWVKPYLPVHKELFEQEDKFGVAEDKNGNVW
                     IGVSKKGVYCLNKKGNVIDSINSVNGLSSDLVYELCIDKYNRLWVVTSEAVHFVNLEN
                     KHVSTLDFRAPYPFGDFWPVLRAEDSLLAVTYNDITCFVNLDKLGEKLQKKKPLITSI
                     KVFETEIPFDFSKPELSLKHSQNFFTIYFSSPHHKEIPSIQYAYKLEGLNIDWVYCGK
                     RQVASYSNVSNGHYRFLVKSTNSNGEWSDAIAIVEINIKAPFWKTAWFISLVVLLALT
                     ILWLLYKRYVARNKKKIIEKTIDYFANSVYGENSVNEICWDIARNCISQLQFEDCVVY
                     LIDEKTNRLVQKAAYGPKNPKGHEIENPIEVEMGNGIVGTVALTGKPLLIADTSKDER
                     YVVDDVARLSELAVPILHENKVIGVIDSEHPQKNFFSHEHLKALSTIASISANKIAEA
                     QAEASAKENEIKLLEINRMLAESQLMALRAQMNPHFVFNCLNSIQECIVTQKYGEASN
                     YLNKFSKLFRMVLNNSGRNLVTLEEEAEVLRLYLELELMRFENSFSYTIHIDEELDQE
                     EIMIPSMLVQPYVENALWHGLMHQEGERKLAISFEKISDEVFGCIIDDNGIGRKKSFE
                     LKQQQSKAKRHESKGLKITKDRLDVLRKQGYHASLEIIDKYDAQQNPTGTKINIELST
                     DLVH"
     gene            4746..5495
                     /locus_tag="PK977_02655"
     CDS             4746..5495
                     /locus_tag="PK977_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF17033.1"
                     /translation="MLKALIVDDEHKARSILHHYLVNFIPEIKKVEQAASVDEALSVL
                     PGLRPDIIFLDVEMPHKNGFDFLTAVTNPVFDVIFTTAYNQYAIQAIRFSALDYLLKP
                     VDPEELKAAVQRHLNKRENEEQRQELYTNLVQNIEKKEVKDFKIAVPSLEGIYFFKVD
                     EIIRLEAEKSYTHIHLTSRKPFIASKTLRHFEDMLEEFKFIRTHKSHLVNPKYIMRIS
                     NDNSFVVLSDGSQVEVSRRKKEEVQGQLKLR"
     gene            5588..6370
                     /locus_tag="PK977_02660"
     CDS             5588..6370
                     /locus_tag="PK977_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17034.1"
                     /translation="MIQIKNYLFAGLAIFTISSCSKNNTDVIPPVTQELKKTIKTSTI
                     NGNISFEFGPEGKPKQFIVERPWGSYRYDFIYEPAKATINVFNIATNQKEETYQASLN
                     DKGHITQLIFDQYDNNGNPTATATTSYSYNAKGLLEMTTSPSSFSKKYYYDTEGNNIK
                     TEYYNGSGQLINYTENDYSDKLDKCPAHNINQFTWQGFFLPALSKKLLNHKKQINAGT
                     NTINFEIGITYELDAEGFVKKGMVDDLNPAGTDWSWTFTFDK"
     gene            complement(6934..7320)
                     /locus_tag="PK977_02665"
     CDS             complement(6934..7320)
                     /locus_tag="PK977_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF17035.1"
                     /translation="MPDVIIKNRRFYNPVDFVFKKLGGTWKIPVLWRLRTNTRRYTEL
                     QQDIAHISQKMLTQTLKELEAEGFVSKKVFAEVPPRTEYSLTPKGKKAIEVITYLRNY
                     GLQLMKEEGIDYDAMIEAEKKNIVKK"
     gene            7429..8193
                     /locus_tag="PK977_02670"
     CDS             7429..8193
                     /locus_tag="PK977_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRF17036.1"
                     /translation="MSNVKEVNQYVHFHGAPTKGKILMVASSPAVSQQTGWPIGFWAA
                     ELTHPLRVFQEAGYEVELVSTEGGALQMDGFSNPTDASGYSAHDVISLGYMQQQWFND
                     MLANTKKLSAINPADYVAIFLVGGQGPMYTFRGNKELEKLFAAFYESGKPSAAVCHST
                     TLLLEAKTSNGELLVKGKTWTGFADAEEEFADQAVGMKIQPYRIETEAKKIDGTNFKV
                     AAPFSSYAIADGNLITGQQQNSGAAAAELVVEQLNK"
     gene            8197..8814
                     /locus_tag="PK977_02675"
     CDS             8197..8814
                     /locus_tag="PK977_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF17037.1"
                     /translation="MNIAIIGTGNVGGALATKWAQKGHHIFLGVNDTANFKGRELLNN
                     PNTSVHSVKEAVQQADVILIATPATVAAEVAESLGDTSDKIIIDAMNVVMGKGPAGFT
                     NTTDAILSHTQSKDVVKCFNTTGFNNMLNPVYGNTAIDVFVAGDSVKGKEVALQLAKD
                     AGFAECYNVGGNDRFQLMEQFAFFWINLAMMQGLGREIGFKLLKR"
     gene            8827..>9658
                     /locus_tag="PK977_02680"
     CDS             8827..>9658
                     /locus_tag="PK977_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRF17038.1"
                     /translation="MIIKKVFNPLAIARYMKTELVVSVLLSAGVYLLYYTGQIEKASL
                     PFSVAVILGSALAIFLAFRNNNAYSRWWEARTLWGGITNNSRIFARQIIANADNAVHT
                     GKAPAELVAAYKKEMIYRQIAYVHALRLQLRGQHNPGEYQYLLDEKEFSEMIRKQNPA
                     NYLLLVQGIRIKEAMNTEILGAFDNISLEPTLAGFNNFQGGCERIRNTPLLRQYHFFT
                     KLFLLVFMIVLPFSLISDFSKMGMPWLMIPVSILISFVFGVMGKVGEVNEDPFENRIT
                     D"
     assembly_gap    9659..9701
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9798..10961
                     /gene="purT"
                     /locus_tag="PK977_02685"
     CDS             9798..10961
                     /gene="purT"
                     /locus_tag="PK977_02685"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRF17039.1"
                     /translation="MHKKILLLGSGELGKELVIALKRLGQTVIAVDSYAGAPAMQVAD
                     EFEVIDMLNGEQLDSVVSKHNPDLVVPEIEAIRTERFYTYEKQGRKVIPSAKAANFTM
                     NRKLIRDLAAKELGLRTAKYFYATTFEEFKNAVGEIGLPCVVKPLMSSSGKGQSVLKK
                     VEDMQRVWDYAITNSRGDIHEVIIEEFISFHTEITLLTVTQQNGPTLFCPPIGHRQER
                     GDYQESWQPVAISENDYKDACMMAEKVTTALSGPGIWGVEFFLTEKGVYFSELSPRPH
                     DTGMVTLAGTQNLSEFELHARAILGLPIPEIKLERAGASAVVLANKLAEQFIITGIEN
                     ALAEKQTDVRIFGKPTTRPYRRMAVALVYDDIQSDINELRKKAKSAADKIAIL"
     gene            11101..11697
                     /locus_tag="PK977_02690"
     CDS             11101..11697
                     /locus_tag="PK977_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HRF17040.1"
                     /translation="MKKVYLLPAILIASVAALVGSCGHNSNADGQTISKDSLIKRGNY
                     LVTTMGCNDCHSPKVMTAMGPALDTTRLLSGHRSDVQLPPANTDALKNGWALFYAEGT
                     AMVSPLGTSYAANITSDATGIGNWTLQQFKTAITKGKWKGMENSRNLLPPMPWENYKD
                     MSDADVEAIFTYLQSTKPVNNVVPAPQLAYSEIKPDKP"
     gene            11709..12380
                     /locus_tag="PK977_02695"
     CDS             11709..12380
                     /locus_tag="PK977_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRF17041.1"
                     /translation="MNKIKIFSTPHKALRKLMAEFSLLAGKTDYANESELQALKQMGN
                     EMFDLLKEHAHIEESLVLTALEAKAPGSSDENIEEHEQLEKVQAELEEWLNSFDGSQS
                     EEEGFQFYLAFSSYHGQYLEHIIHEETNTQELLWKYLLDEELMGLQMQILKSIPFDVF
                     LVWGKHITPAQTRKENLAMLTGMKMMLPAPAFEKIVSMVQASVSANEYYELMTELGKL
                     PAKAA"
     gene            complement(12459..12980)
                     /locus_tag="PK977_02700"
     CDS             complement(12459..12980)
                     /locus_tag="PK977_02700"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF17042.1"
                     /translation="MIFIYILAALIGALLIGAYLMPKSYNVEKSIIINKSPAAVRNFV
                     ADLNHYSQWNPWQQSDPTATHTITGQAGTPQHKYAWEGKKVGTGSLTLISSDDKHIHF
                     DLEFVKPWKSKAKDNWLFEAWGDGNETKVTWQNSGELPWPMARLMGPMINKNLNHQFG
                     VGLENLKKMVESH"
     gene            complement(13042..13620)
                     /locus_tag="PK977_02705"
     CDS             complement(13042..13620)
                     /locus_tag="PK977_02705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF17043.1"
                     /translation="MKVWPLLLVIVSACGTGYRYYDSVRYKTGHDSSFVYSLPFATGK
                     KVFVVQGYESMFSHKGSKALDFKVKSGTKIFAARNGVVIGARGDSDKRGLKQENLADG
                     NYIFIQHADGSVAQYWHFKKDGVLVQVGDTVTSGRLIGFSGNTGYSAFPHLHFEVRGY
                     DQNGNYTDIPTRFYTQQGIIYLQPGKFYRTFR"
     gene            complement(13643..15346)
                     /locus_tag="PK977_02710"
     CDS             complement(13643..15346)
                     /locus_tag="PK977_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HRF17044.1"
                     /translation="MKRLAAGLLLLAATPVLSFSQQEYKGTVSLIPKGPDFNAAYYVN
                     KLSMKVDAGGTAVLTANLRFGPKTEPGDYIAFTGNISGTLQGGELSLSGSLNQAMQDG
                     KRFVEEAVTTRVTGQKEGDKITGTFYMRYDGEETSTMTFTLNPGELAPELLFPLGSSP
                     KVFDKGWLFGASFKITSKDNEEIDLTDKIEWSGTASFEPDKGASSRPVFNNIGENKII
                     LTVEYEGKKYSKEYKVVTVSSNLYARVGSKSLCQGDSHGTPADPLVCIGPVITGNGNV
                     LIDGMPAACEGDRGVHAACGGPNNFVIVDGDPEVLINGKKAAKLGTLTRHCGGMGRIV
                     STDGGSGTTDLLKGLSDDISITKNGQKIPNNSKPEKGMTLKTGPKGLLMMSPDRNSVM
                     MIMPNTTAVITGADGNDLVIEINEGTLFVNGEKANDNKNLVIESINEKLLRKGTRFLY
                     CHDKDSSRLIVYEGEVEVTLKKENRTLLVPAGKVYFNDFKNPYVIADTLEFQSAGELM
                     KIPKDSAYWQLPKDDAATKDAGSNQPAGFLEMIKKYWYFAAGGVVLILLMVYMGKRKK
                     S"
     gene            15722..>16927
                     /locus_tag="PK977_02715"
     CDS             15722..>16927
                     /locus_tag="PK977_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRF17045.1"
                     /translation="MKTKFKVFWPIIALLVLNACQKEKELVNPVQPIPVPVPTVDRNA
                     LVITTDNTGVFNASNALTGASKWTYSTPGMTAFAPPAVSSKYTVFADAATSSVYCMDT
                     RNGYILWEKDGINSSYLISPLLVDDNLYIPNDNMLQVYDPENGELIRELPVIPYPHSL
                     NYVKDLLIYSNCGGHLVAMSTDGVQQWEYMSPAGCYHNNPAIANNTIYILSSSGYLSA
                     IDVTTGAELWTKNMAAYTYEASLVYDDGLLFAIDHKQADKLYAFSAATGDLVHTYMLP
                     AGESCNMWLTPAVKSGKMYFMTEEGTLVAYSVEDELVLWQKQFSVEGRNINVQIKNGN
                     RVERTESHSDMTSVIMANNWLYFGAGKFVYAADINSTTYWQAPLNGFIYNSPVVLTEF
                     GNVFRAGTAG"
     assembly_gap    16928..17046
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17047..17449
                     /locus_tag="PK977_02720"
     CDS             <17047..17449
                     /locus_tag="PK977_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRF17046.1"
                     /translation="SSMKLDLAALRPVNVQRAKEGFKCYDNQPLTYWTTALAGEVGEL
                     CNMIKKMQRVEKGGMDGGSSYSAKDITKEMLKEEIGGIAIYLDLLASLMDISLEEAII
                     DTFNSKSDKYGFSQRIATGLNQRISEVLSEK"
     gene            17612..18274
                     /locus_tag="PK977_02725"
     CDS             17612..18274
                     /locus_tag="PK977_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17047.1"
                     /translation="MQKSVLSIMVVAALLLLASCSKDDNGGQLSYQLKTKNPNATVSR
                     TSSGSVTWTSGSAYADKIEFEAEKDDDNEVEYESRVARRIDLFAPVSQLGNISLPPGR
                     YEEIEIEVDLAPSITDTAFILRGSFTNNIGYTVPVVFYINELLEFKAEAEDISIGSAA
                     DITALTTLDLSQLTTGITESMLSSATLTNGVLVISATSNAALYSVILSNINNIDDVEL
                     DD"
     gene            18353..19174
                     /locus_tag="PK977_02730"
     CDS             18353..19174
                     /locus_tag="PK977_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRF17048.1"
                     /translation="MSAYFNDTFIAGEEVKLHVADLSIQRGYAVFDFCRTVNRKPLFI
                     NDHLERFFASAAAMHLPVNKTKEELIHIIGQLIQQSSLPEAGIRIMLTGGYSPDTYTP
                     AVPNLLITCNPVKTATEADFEKGVSVISFEHQRELPHIKTINYTMAVWLQPLVKEKKA
                     DDVLYHHNKIITEFPRSNVFVVTKDNRLLTPAHNMLKGITRKQVLSLAAAILPVEERD
                     IHREEVYEAAELFLTSTSKKLMPVVRIDDIVIGEGKPGVITRELYRRFLEKEKNQ"
     gene            complement(19216..>19908)
                     /locus_tag="PK977_02735"
     CDS             complement(19216..>19908)
                     /locus_tag="PK977_02735"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRF17049.1"
                     /translation="APDKISGGHTHGGYSKGIVVHEDFVLHIADNLPLDAAAPLLCAG
                     ITTYSPLRHWKVGKGTRVGILGLGGLGHMGVKLAAAMGAEVTMLSHTPSKEKDAKRLG
                     AHKFVLTSDEAQVKSVMGYFDFILDTVSADHDYNFYLGLLRTSGVMVCVGAPSAPATV
                     PAFNLIFGRKSIAGSLIGGLPETQEMLDFCAEHNIVSDIELIRMDQINEAYERLLKSD
                     VKYRFVIDMSTL"
     assembly_gap    19909..20061
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20062..20253)
                     /locus_tag="PK977_02740"
     CDS             complement(<20062..20253)
                     /locus_tag="PK977_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRF17050.1"
                     /translation="MPATKAYGAHSATTPLVPLTIERREPHPHDVQIDIQYCGVCHSD
                     LHTVRNEWHNAIYPCVPGHE"
     assembly_gap    20375..20391
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6018 a         4160 c         4284 g         5615 t
ORIGIN      
        1 gcagcagaag gggattgcct gcgttgcaat ggtgggcaaa tgcatgtcat tacagtatgg
       61 gcaagcatcc ggcagcgggc atatatgatg atcagcgtta tctggatctt ttcccgcttt
      121 tgtttgacaa tgtaaaaata ttgttgcata agggatgtaa cctgggagca gaaaattatg
      181 aagcatgtaa aagaatatca acggggtgaa ggcggcatcg ttattatgag caatgatgca
      241 catatagaag taagccgccg caaaaaagaa cagttcctgg aaaaaataaa agagatttat
      301 aagtattaag aagtcactgt atattttatc acaggaataa aactgacatg cagccggaat
      361 ttgtttttta aggggaataa tattattggg caggcttctt atactttctc cagcttctca
      421 ggtacattat cccttttgat gtctgaaatc ttgttggcac ttgttgccat tgtccgccgc
      481 ctgagcgcca taccaggaaa tgcaggtgcg gtacaaacgc ataacctgtt ttgccactaa
      541 gtgcaatggg ctgtcctttt ttcaccgtgt cgccaatatt aaccagaacg ccgttatgtt
      601 gcaggtgcca gtaaccggca cgggagccat cggcatgctg tataacaatg ttgtttcctt
      661 cagaccggta tttcctgttg agaccgcctt tctttccgtc ttctttagcc cttaccacaa
      721 taccgtctct tgctgcggtg atggtatcac ccttcttcat attaaagtca agagccgctc
      781 tttctttatg cgtaaaacgg ctgaagtaac cctgaatcac ccggtatttc tttctttccg
      841 gaaagggaag agcatacaca taacttgtat catctttaag tatccccttt tgcaacagtt
      901 ttacctgttt gcgcaacggg tttttgctca ctgagcagcc caccatcaaa aggataaata
      961 caagcagcag gaacttcatc ctgtaaattt aagcccctgc ccgatccctg acaatttgtt
     1021 aatgccgatg gttgtttcgg cgtaaccatt cacaaatcgg ttaattgtct tttgctggtt
     1081 ggctccaata tttatatctt aatccggtaa tcgggtcatg aaattaaagc tgcttatata
     1141 ctgcttcttt gttctgtcaa acttccagct gcaaggtcaa tttcttttct ctagcctggg
     1201 aacaaaggaa ggtatgtctt ccagggatgt aaaagatgcc tgtaaggaca aggatggttt
     1261 tatttggttt ggtacaagca atggactgaa caggtatgat ggcagcggat ttaagattta
     1321 tagtcgccag tataaaaaca gccgcagtct taccagcgat attattaact gcgtttccga
     1381 agtggatggc aatatctgga taggcacacc aacaggttta tttgtattag acaaaaaaat
     1441 ggataccatc cggccggtac tttttactcc aaacagatct acaggggtgt taaagtttga
     1501 gaaggatttt gaaaataaaa catggataat tactgaccac agaattttca ttcttaacaa
     1561 aggcatcccg gagccgatag agaaaacata tcccggaact gccagactgg tcacatataa
     1621 gaccttcaat acggtggaaa gagattcttt ccggaaagga atatggttca gtgcagacaa
     1681 caacctgttt ttttttcatc agcaatcagg gctttttttt tcacaggaaa acaaccccta
     1741 caactggtca atatttcaaa caggtcatgc tataagtttg gcagttgatg ctacagggaa
     1801 aatctggttt ataacatcac ttcttcccta tttacaactt tttgatttta ccactaatac
     1861 gtcatcccgg attatggatt cgggtttcga gtacccaaaa gtgctgaaat ccgtcaatgc
     1921 agttggcaaa ctaatgaccg acagcaaagg aagaatatgg ataagcacat ggggcggcga
     1981 actgttaatg aacattggtc cgaataaaat aattaacgta tctgagcaat caattacagg
     2041 gtataagccg gctttcaccg tatttgaaac tatttacagt gatgatgccg gtaacgattg
     2101 gctgctcggc aaggacgggg caaataaaat ttggggcaat tctgtttttg attatgtgac
     2161 acagcttann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatccc cagataatta
     2341 cagggcaaac cgtttttttc atgcaatatt ttacaggaac gaatggtggt ttgctacggg
     2401 cgatggaata ttgattttaa atccggtcag taaccggttt agaaaattta cccattacgc
     2461 aaaaggctat gaaataaaaa acaagtcagt taccaacttg ctggttgaca aaaaaggtaa
     2521 actctggttt tccgtctgga atgatgcact ttaccggtat gaccctgtaa ccagtcagac
     2581 tgtaagatat gacgggcatg atgccgggca aggcgatatc cgtacgctta attgttcatc
     2641 ggcttcagaa gatatcgaag gtaacatctg gattacatcg cagttgctac ggaaatttga
     2701 aatcactaaa gagaagtggg ttaagcctta tttgcctgtg cataaagaat tgtttgaaca
     2761 ggaagataaa ttcggtgtag cagaagataa aaacggaaat gtctggattg gagtcagtaa
     2821 aaaaggtgtt tattgtttga ataaaaaagg taatgtaatt gattcaatta actctgtaaa
     2881 tggcttaagc tctgatttgg tttatgaatt atgtattgat aaatataatc gtctatgggt
     2941 agtgacatcc gaggctgttc attttgtaaa cctggagaac aaacatgttt ctacactcga
     3001 tttcagggcg ccataccctt ttggagattt ctggccggtg ctgagagcag aagatagttt
     3061 actggctgtt acctataatg atataacctg ttttgtaaac ctggataagc tgggagaaaa
     3121 attgcagaaa aaaaaaccgc ttattacttc tatcaaagta tttgagacag aaatcccttt
     3181 tgatttcagt aagccggagc tgagcctgaa acacagccag aattttttta cgatttattt
     3241 ttcatcaccc catcataaag aaattccttc catacaatat gcttacaaac tggaaggcct
     3301 gaatatcgac tgggtgtatt gcgggaaaag acaggtggca tcctactcaa atgtcagcaa
     3361 cggacattat aggtttcttg taaaatcaac caactcaaac ggcgaatgga gcgatgctat
     3421 tgctattgta gaaataaata taaaagcacc attctggaaa acagcctggt tcatatcact
     3481 tgttgtctta ctggccctaa ccattctttg gctgctatat aaaagatatg ttgccagaaa
     3541 taagaaaaaa attattgaaa aaacgattga ctactttgcc aattctgttt atggtgaaaa
     3601 ttccgtaaat gaaatctgct gggacattgc acgcaactgt atatctcagt tacagtttga
     3661 agattgtgtg gtgtatttga ttgatgaaaa gacaaaccgg cttgtgcaaa aagcggccta
     3721 cggccctaaa aacccgaaag gccatgagat agaaaatccc attgaggttg aaatgggcaa
     3781 tgggattgtc ggcactgttg ccttaaccgg aaaaccctta ttgattgcag atacttcaaa
     3841 agatgaaaga tatgtagtag atgatgtggc caggttatca gagcttgccg ttcccatatt
     3901 gcatgaaaac aaagtgattg gcgtaataga ctcagagcat ccacaaaaaa atttcttttc
     3961 ccacgaacat ctgaaagcgc taagcactat tgcttcaatc agtgcgaata aaattgcaga
     4021 agcacaggca gaagcatcgg caaaggaaaa tgaaataaaa ttgttggaaa taaacaggat
     4081 gctggccgaa agccagctga tggctttgcg agcacaaatg aacccgcact ttgttttcaa
     4141 ctgccttaac agtatccagg aatgtatcgt aacacagaaa tatggtgagg caagcaatta
     4201 tctcaataaa ttttccaagc ttttccgcat ggtgctgaat aactcaggaa gaaacttagt
     4261 tactctggaa gaagaggcag aagtgcttcg gctgtacctt gaactggaac taatgcgttt
     4321 tgaaaacagc ttcagctaca caattcatat agatgaagaa ctcgatcagg aagaaattat
     4381 gattccctcc atgctcgttc agccttatgt agagaatgca ctctggcatg gattgatgca
     4441 ccaggaggga gaaagaaaac ttgcaatatc gtttgaaaag atttcagatg aagttttcgg
     4501 ttgcataatt gatgataacg gtattggacg caaaaaatct tttgaattga aacaacagca
     4561 gagtaaagca aagcggcacg aatcgaaagg attaaaaatt actaaagaca ggctggatgt
     4621 tttacgtaaa caaggttatc atgcttcact ggagattatt gacaagtacg atgcacagca
     4681 aaacccgacc ggcacaaaaa tcaatattga actttcaacc gatttagttc attaaaatgt
     4741 tctttatgct taaagcatta attgtagatg acgaacacaa ggccagaagt attctgcatc
     4801 actacctggt gaattttatt cccgaaataa aaaaagtaga acaggctgct tcggtggatg
     4861 aggcattgtc tgtgttgcca ggtttaaggc cagatataat tttcctggat gtggaaatgc
     4921 cgcataaaaa cgggtttgat tttttaactg ccgttaccaa tcctgttttt gatgtaattt
     4981 ttacaacagc gtataatcaa tatgccattc aagccattcg ttttagcgct ttagactatt
     5041 tgcttaagcc tgttgatccg gaagaattga aagcagcagt gcaaaggcac cttaataagc
     5101 gggaaaacga ggaacaaagg caagaattgt acactaatct ggtccagaat atcgagaaga
     5161 aggaggtgaa ggattttaaa attgctgttc cttcgctgga aggaatttat ttctttaaag
     5221 tagatgagat aataaggctg gaagccgaaa aaagctatac ccacatccac ctcactagcc
     5281 gcaaaccttt tattgccagt aaaacactta ggcattttga ggatatgctg gaggagttca
     5341 aatttatccg tacccacaaa tcacatttag tgaatccaaa gtatattatg cgcatcagca
     5401 acgacaacag ctttgtggtt ctttctgatg gttcacaggt tgaagtttcc agaagaaaaa
     5461 aagaagaagt acaggggcaa ctgaaacttc gctgaccgtt tgcttaacga ggcccgccgt
     5521 ttattgattg gatttgtatc caccggctgg tagatattta atttgtatcc taataaaaaa
     5581 taaaattatg atacagatta aaaactatct ctttgcaggc ctggctattt tcacgatttc
     5641 atcgtgcagt aagaataata ctgacgttat accgcctgta acacaagaac taaaaaaaac
     5701 cattaaaacc agtaccatta acggcaacat tagttttgag tttggtccgg agggtaagcc
     5761 caaacaattc atcgtggaaa gaccatgggg tagctatcgt tacgacttta tctatgagcc
     5821 tgctaaagca actattaatg tttttaacat tgctactaat cagaaggagg aaacttacca
     5881 ggcttctctc aatgacaaag gacatataac acaactgatt ttcgaccaat atgacaataa
     5941 tggaaatcca acagcaactg ccaccacaag ctacagttat aatgccaagg gattgctgga
     6001 aatgacaacc agcccttctt ctttttcaaa gaaatattat tatgatacgg agggaaacaa
     6061 cataaaaaca gagtactaca acggcagcgg gcagttaatt aattataccg aaaatgacta
     6121 tagtgacaaa ttagataaat gcccggctca taacataaac cagtttacct ggcaggggtt
     6181 tttccttccg gctctttcta aaaagctttt gaaccacaaa aaacaaatta atgctggcac
     6241 aaacacaatc aactttgaaa tcggaataac ctacgaactg gatgctgaag gttttgtgaa
     6301 aaaaggtatg gttgatgatc ttaaccccgc tggcacagac tggagttgga cgtttacgtt
     6361 tgataaataa tgcacaccta atcattaacc aggacccggt tctcttaggc cgggttttcc
     6421 ttttattgtt tcattttcgc cacttaatat ttgctttagt attactaatt atctgtggtt
     6481 atttttttgg ggttttaata ttttttctta aggcaggctt caatgtacag gtagaaatca
     6541 tcggccccgg aagcctctgt tttaccgctt acttcattga ggggtatgtt ttttataccg
     6601 gtgtactgta aaaactattt cagacccata ccatccctgc tcttttttac ataggctgaa
     6661 aatgaaaagg gcttacatgc acttgctttg cttctgcatc catccaattt gaggggaaaa
     6721 atactcttgc aggtagctta tagcctgatc aattctttcg tagtctgtca ttgttttaaa
     6781 tttactacac aaaaattgca aacactactt atgcatacaa cccgtttctt gcggaaaaca
     6841 cggctatgtt tttttacatg tgatatggct gaaaccctct gatttgcaga gtcaaacaag
     6901 ggtaacagaa gtataaaaga tttcagcgag atgttatttt tttacaatgt ttttcttttc
     6961 cgcttcgatc attgcatcgt aatctattcc ttcttccttc atcagttgta acccgtagtt
     7021 ccgcaggtag gtaattactt ctatggcttt tttccccttt ggtgttaagc tatattcagt
     7081 tcggggcgga acctccgcaa ataccttttt ggaaacaaaa ccttctgcct ccagctcttt
     7141 taatgtttgc gttaacattt tctggctgat atgggctata tcctgttgta attcggtata
     7201 acgccttgtg ttcgtacgca gcctccaaag cactggtatc ttccaggtac cgccgagttt
     7261 tttgaaaaca aaatccacag gattgtaaaa tcgtctgttt ttgattatta cgtcaggcat
     7321 atgctaaaaa tacagcttac gcaccaaaaa gttcgttaca gccttattgg tgttttacaa
     7381 ggttgtacta tccggctgca acaactttac ggtacaaaaa tttatcacat gtcaaacgta
     7441 aaagaagtaa atcagtatgt gcattttcat ggagccccca caaaaggtaa aatactaatg
     7501 gtagccagca gcccggctgt ttctcaacaa accggctggc cgattggttt ctgggcagcg
     7561 gagctaactc atccgttgag ggtatttcag gaagcgggat acgaagtgga actggtgagc
     7621 actgaaggcg gtgctttgca aatggacggc ttcagtaatc ctaccgatgc cagtggttat
     7681 tcagcccatg atgtgatctc actgggatat atgcaacagc aatggttcaa tgacatgctc
     7741 gccaacacaa agaaattatc agccataaac cctgccgatt atgtggctat ctttttagtg
     7801 ggcggccagg gtcctatgta cactttccgg ggtaataaag aattggaaaa actttttgcc
     7861 gccttctatg aaagcggtaa acccagtgcc gctgtttgcc actcgactac cttattgctg
     7921 gaagcaaaaa catccaacgg tgaattattg gttaaaggaa aaacatggac tggctttgct
     7981 gatgctgaag aagaatttgc agaccaggca gtaggaatga aaattcagcc ttaccggatt
     8041 gaaacggaag caaagaaaat tgatggcact aattttaaag tggctgcgcc tttttcttct
     8101 tatgccattg cagacggaaa cttaataacc ggccagcaac agaacagtgg cgctgctgcg
     8161 gctgaactgg ttgttgagca attaaacaag taaaaaatga atatcgcaat catcggcact
     8221 ggtaatgtag gcggtgcctt agctacgaaa tgggcacaaa aaggccatca cattttcttg
     8281 ggtgtaaatg atacagccaa cttcaaagga agggaattgt tgaacaatcc caatacctcc
     8341 gttcattccg ttaaagaagc ggtacaacaa gccgatgtta tacttatcgc caccccagca
     8401 acggtggcag cagaagtggc agaatcgctg ggagatacca gcgataagat catcattgat
     8461 gccatgaatg tggtgatggg aaaagggcct gcaggtttca ccaataccac agacgccata
     8521 ctatcacata cacaatcaaa ggatgtggtg aaatgcttca acaccaccgg gtttaataac
     8581 atgctgaacc cggtttatgg taatacagcg atcgatgtgt ttgtagcagg tgatagtgta
     8641 aagggaaaag aagtggcctt acaactggct aaagatgccg gctttgccga atgttacaac
     8701 gtaggaggaa acgaccgttt tcaattgatg gaacaatttg ctttcttctg gatcaacctg
     8761 gccatgatgc agggactggg aagagaaatt ggttttaaac tcttgaaacg ataacacccc
     8821 tgcagtatga taataaaaaa ggttttcaat ccgctggcga ttgcacgata catgaaaacg
     8881 gaactggtag tttcagtttt attatcagcc ggtgtttact tgttatacta tacagggcaa
     8941 atagagaaag caagtcttcc tttttccgtt gcagtaatct tgggtagcgc attggctatc
     9001 tttctcgcgt ttcgaaacaa taatgcctat agccgctggt gggaagcaag aacactatgg
     9061 ggaggcatta cgaataacag ccgcattttt gcccggcaga tcattgccaa tgcagataat
     9121 gcagtgcaca ctggaaaagc accggcagaa ctcgtagctg catacaaaaa ggaaatgatt
     9181 taccggcaaa ttgcatatgt ccatgcactg cgattgcaat taagagggca acacaatcct
     9241 ggtgaatacc agtatctttt ggatgaaaaa gaattttcag agatgatccg gaaacaaaac
     9301 cctgccaatt atcttttact ggtgcagggt ataaggatca aagaagccat gaatacagaa
     9361 atactgggag cttttgataa tatcagcctt gaacccacac tggccggttt caacaatttc
     9421 cagggagggt gtgaacgcat acgcaataca cccctgctgc ggcaatatca ttttttcacc
     9481 aaattattcc tgctggtgtt tatgatcgtt ttgccttttt cattaataag tgatttcagt
     9541 aaaatgggaa tgccctggtt aatgatacct gtaagcatac taatttcctt tgtatttggg
     9601 gtaatgggta aagtaggcga agtgaatgaa gacccgtttg aaaatcgtat taccgatgnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagaaatg ctgggagaat
     9721 cagttcctcc caaaatgctt cctgaaaaag gattccttta ttaaaatcgc tgttgtatta
     9781 attctttatt ttgtggcatg cacaaaaaaa tactcctgct cggcagtggc gaactcggta
     9841 aggaactggt cattgcttta aaaagactgg gacagacagt aatagcagtg gatagttatg
     9901 ccggtgctcc ggctatgcag gtagctgatg aatttgaagt aattgatatg ctcaatggtg
     9961 agcagcttga ctcggtagtc agtaagcaca atcccgacct cgttgtgcct gaaattgaag
    10021 caatccgcac agaacgtttt tatacatatg aaaaacaggg aagaaaagtg atacccagtg
    10081 ccaaagccgc caactttact atgaacagga aactgatcag ggacctggca gcaaaagaac
    10141 tcggcctaag aacagcgaaa tatttctatg caacaacatt tgaagaattc aaaaatgcag
    10201 tcggggaaat tggccttccc tgcgtggtaa aaccgctgat gtcttcttcc ggaaaaggac
    10261 aaagtgtatt aaagaaagta gaagatatgc aaagggtgtg ggactatgcc atcaccaact
    10321 cccggggaga tattcacgaa gtgatcattg aagaatttat ttcatttcat actgagatca
    10381 cattattaac ggtaacacag cagaacggac caaccctttt ttgcccaccc attggtcatc
    10441 ggcaggaacg gggcgactac caggagagct ggcaacctgt tgccatcagt gaaaacgatt
    10501 ataaagatgc ctgtatgatg gcagaaaaag taaccactgc cttatctggt cccggcatct
    10561 ggggtgtgga atttttcctg acagaaaaag gagtgtattt ctccgaacta agtccacggc
    10621 cacatgatac cggcatggta acactggcag gaacacaaaa cttgtcagag tttgaattgc
    10681 atgcaagggc aattttgggt ctccccattc ctgaaataaa actggaacgg gcaggtgcca
    10741 gcgcagttgt gctggcgaat aagttagctg agcaatttat cattacagga atagagaatg
    10801 cattagcaga aaaacaaaca gacgttcgca tattcggcaa acctacaaca agaccctatc
    10861 gaagaatggc cgttgcactg gtgtatgatg atatacaatc cgatatcaat gaattacgaa
    10921 agaaagccaa atctgcagct gataaaattg caattctgtg aatcaatttt ctgctaacaa
    10981 aaattaacac ccactcctta cctcctcttt gtttaccgct tgtcactacc acataaagat
    11041 gtggcatgct gttattgtaa cgataatagc tttgtgaccg ttaaaccaaa aaccttaatt
    11101 atgaaaaaag tttatttatt accggcaata ttgattgcct ctgttgcagc gctggtagga
    11161 tcatgtggtc ataacagtaa tgctgatgga caaaccatca gcaaggattc attgatcaaa
    11221 agaggaaatt atttagtaac aacaatgggt tgcaacgatt gtcactcacc taaagtaatg
    11281 acagcaatgg gccccgcatt ggatacgacc cgtcttttat ccgggcatcg tagtgatgta
    11341 cagctaccgc ctgccaatac agacgcatta aagaacggct gggccctttt ctatgcagag
    11401 ggtactgcca tggtaagtcc gctgggtact tcttatgcag ctaatattac ttcagatgct
    11461 acgggtattg gcaactggac attacaacag tttaaaacag caataacaaa aggaaagtgg
    11521 aagggaatgg aaaacagccg aaatctatta ccccccatgc catgggaaaa ttataaggac
    11581 atgagcgatg cggatgtaga ggctattttt acttacctgc aatcaaccaa acctgtaaac
    11641 aatgtggtgc ctgcaccgca actggcttac agtgaaatta aacctgataa accttaaaac
    11701 aaccaactat gaacaagatc aaaatcttta gtacgccgca taaagcgctg cgtaaactaa
    11761 tggccgaatt ttctttgctg gctggcaaaa ctgattatgc caatgaaagc gaattacaag
    11821 cccttaagca aatgggcaac gaaatgtttg atttattaaa agaacatgcc catatcgaag
    11881 agtctttggt attaactgca ttggaagcca aagccccggg ttcatcagac gagaatattg
    11941 aagaacatga gcagttggaa aaagtgcagg cagaactgga agaatggctc aacagttttg
    12001 acggttctca gtcagaagag gaagggtttc agttttatct ggccttctct tcctaccatg
    12061 gccaatacct ggaacatatt atacatgaag aaaccaacac ccaggaattg ttatggaaat
    12121 acttattaga tgaagaactg atgggcttgc aaatgcagat attaaaaagc attccgtttg
    12181 atgtattcct ggtttggggt aaacatataa cccctgcaca aaccaggaaa gaaaatctcg
    12241 ctatgctaac agggatgaaa atgatgttgc cggcccccgc ttttgaaaaa attgtttcaa
    12301 tggtacaggc atctgtatcg gccaacgaat attatgaatt aatgactgaa ttaggaaagc
    12361 tccctgctaa agcagcctag tataaaagta aagccatgaa aaaagccggt ctgtgatttt
    12421 tcacaaccgg ctttttattt atatgataaa cagtttgatt aatggctctc caccattttc
    12481 ttcaggtttt ccaaaccaac accaaattga tggttgaggt tcttgttgat catcggtccc
    12541 atcagtctgg ccatcggcca gggaagttca ccactgtttt gccaggttac tttagtttca
    12601 ttaccatctc cccatgcttc aaaaagccag ttatcttttg ctttgctttt ccagggcttt
    12661 acaaattcaa gatcgaaatg aatatgcttg tcatcactgc tgatcagcgt aagactgccg
    12721 gtgcctactt tttttccttc ccatgcatat ttatgttgcg gtgtgccggc ctgaccagtg
    12781 atcgtatggg tggcagttgg atcactttgc tgccaggggt tccattggct gtaatggttc
    12841 agatctgcaa caaaatttct tacggctgcg ggtgatttgt taatgataat tgatttttct
    12901 acattataag atttaggcat caggtaagcc ccaatcaata atgctccgat caaagcggca
    12961 agtatatata tgaaaatcat ggtggctgtt ttaggagagt caagttaacg ataaaacaaa
    13021 gccccgccaa tgattattat actaccggaa agtgcgataa aacttaccgg gttgcaggta
    13081 aatgattccc tgttgagtat aaaatcgggt gggaatatca gtatagtttc cgttttgatc
    13141 atagcccctc acttcaaaat gcaaatgagg aaatgccgaa tagccggtgt tgccactaaa
    13201 gccaatcagc cggccgcttg ttacagtatc ccccacctgc actaatacac catctttttt
    13261 aaaatgccag tactgggcta cagaaccatc agcatgctga ataaaaatat aattaccatc
    13321 tgccaggttt tcctgcttta gccctctttt atcagaatca cccctggcgc ctattacaac
    13381 accgtttcgg gctgcaaaaa ttttagtacc gctttttact ttgaaatcaa gcgccttgct
    13441 tcctttgtgg ctaaacatac tttcataacc ctgcactaca aaaactttct tgccggttgc
    13501 aaagggaagg gaataaacaa aactgctgtc atgccctgtt ttgtacctga cagaatcata
    13561 atacctgtac cccgttccgc aggcggatac aatcaccaat aacaatggcc atactttcat
    13621 tcactgaatt tacaacttct gttcaggact ttttacgttt acccatgtat accatcaata
    13681 gaattaacac aacgccacct gcagcgaaat accagtattt ttttatcatt tctaaaaagc
    13741 cggcgggttg attgctgcct gcatcttttg ttgctgcatc atcttttggt aattgccagt
    13801 aagcactgtc tttcggaatc ttcattagct ccccggctga ttgaaattct aaagtatcgg
    13861 ctataacata aggattttta aaatcattga agtacacttt tcctgccgga acaagtaaag
    13921 ttcggttctc cttcttaagt gttacttcca cttctccctc ataaacgatc agccggctgc
    13981 tgtccttgtc atggcaataa agaaaccttg tacccttacg cagcagtttt tcattaatac
    14041 tttcaatcac caggttttta ttatcgtttg ctttttcccc gtttacaaac aatgtgccct
    14101 catttatttc tataacaagg tcatttccat ctgcaccggt aattactgct gttgtattcg
    14161 gcataatcat catcactgaa ttccggtcag ggctcatcat cagtaatcct ttgggcccgg
    14221 ttttgagtgt catgcctttt tcgggcttac tgttgtttgg gatcttctgc ccgtttttag
    14281 taatactgat atcatcagat aatcctttta ataaatcagt agtacctgat ccaccatcgg
    14341 tggaaacaat cctgcccatt ccaccacaat gacgggtaag agtgcccagt ttggctgctt
    14401 tctttccatt gatgaggact tcggggtccc cgtcaacaat cacaaaatta ttaggcccgc
    14461 cacaggcagc atgcacacca cggtctcctt cacaggcggc cggcatacca tcaatcagta
    14521 cattgccatt tccggtaatt acagggccga tgcagaccaa agggtctgca ggggtaccat
    14581 gcgaatcgcc ctggcacaaa gatttactgc ctactcttgc atataaattt gagcttacag
    14641 ttaccacttt atattcttta ctgtatttct ttccttcgta ttcaacagtc aggatgattt
    14701 tgttttcacc gatattatta aaaacaggcc ggctgctggc tcctttatca ggctcaaaac
    14761 tggcagtgcc gctccattca attttatcgg tgagatcaat ttcttcattg tctttactgg
    14821 ttattttaaa ggaggcgcca aagagccatc ctttgtcaaa tacttttggg ctgctgccga
    14881 gcggaaacaa taactcagga gcaagttctc cgggattcag cgtaaaggtc atggtcgaag
    14941 tttcttctcc gtcataacgc atataaaaag tgccggtgat cttatctcct tctttctgcc
    15001 cggtaactct tgtggtcact gcttcttcta caaacctttt tccatcttgc atggcctggt
    15061 tcaatgaacc gctaaggctt aattcacctc cctgcagtgt acctgatatg ttgccggtga
    15121 aagcgatata atcgccgggc tctgttttgg gtccaaacct gaggttagca gtgagcactg
    15181 ccgttcctcc agcatcaact ttcatactta atttattgac ataataggcg gcattgaaat
    15241 ccggtccctt ggggattaat gatacagtgc ctttgtattc ttgctgggaa aaagacaaaa
    15301 cgggtgtggc tgccagcaac aacagacctg ctgcaaggcg tttcataaaa atgattttat
    15361 gtgaagttgc aacagaaatc tatcctgtta aatgatattc ttcattggta gcaatgatta
    15421 tgatacaggg tccgctaaaa aatcaataaa ggatattcgg ggattaaata aacactgaaa
    15481 tagttattgc aatagaaata aatatttgga atcaccttgt ctcttgtcgt accttaacaa
    15541 tgcaaatgag agtgatggac aaatagccat acaccggacc ggggattgcg gcccggcgct
    15601 tatcgcacaa catcctgttc tgcggcaaat ccaaacaaga tatatgcttt agacttgata
    15661 aacagcaata cacagttgcg tatattactt ctttatcagg ccaacattta aaactagttt
    15721 tatgaaaacg aagtttaaag tgttctggcc gataattgcc ctattggtcc tgaacgcttg
    15781 tcaaaaagaa aaggagttgg tgaatcctgt tcagccaatc cctgttccgg ttcccactgt
    15841 agatagaaat gcattggtta ttaccactga taatacaggt gtttttaatg ccagtaatgc
    15901 attaacggga gcttcaaaat ggacttacag cactccggga atgaccgcct ttgcacctcc
    15961 tgcagttagc agcaagtata ctgtctttgc tgatgcagct acttcttctg tatactgcat
    16021 ggatacaaga aacggttaca ttttatggga aaaggacgga ataaattcct catatcttat
    16081 ttcaccgctg ttggtggatg acaacttgta tattcccaat gataatatgc tgcaggtata
    16141 tgacccggaa aacggggagc tgatcagaga gcttcctgtt attccttatc ctcactcttt
    16201 gaactatgtg aaggatttgc tgatttacag caactgcgga ggacacctgg ttgccatgag
    16261 caccgatgga gtacagcaat gggaatatat gtcacctgcc ggatgttatc acaataaccc
    16321 cgccattgcc aataatacta tttatatact ttccagtagt ggatacctga gtgccatcga
    16381 tgtaacaaca ggggcagaac tatggactaa aaatatggct gcctacacat atgaagcatc
    16441 actggtttat gatgatggcc tgcttttcgc catcgatcac aaacaggcgg ataagctcta
    16501 cgctttcagc gccgccaccg gtgacctggt acacacttac atgctgccgg ctggtgaaag
    16561 ctgcaatatg tggctgacac ctgcagtaaa atccggtaag atgtacttca tgactgaaga
    16621 aggaacattg gttgcttatt ctgtggaaga tgaactggta ttgtggcaga aacaattcag
    16681 tgtagaaggc cgaaacatta atgtacagat taaaaatggt aacagggtag aaagaacaga
    16741 atcacacagt gatatgactt ctgtgattat ggccaataac tggttgtatt tcggcgcagg
    16801 caagtttgtt tatgctgctg atatcaacag cactacctat tggcaggcac cgcttaatgg
    16861 gtttatttac aattcaccgg tagtattgac agaatttggt aatgtatttc gtgccggaac
    16921 ggctggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnatca tcaatgaaac tcgatctcgc tgccctgagg cccgtcaatg tacaaagggc
    17101 caaagaagga tttaagtgtt acgataacca gccccttacc tactggacca ccgcccttgc
    17161 cggcgaagta ggtgaactgt gtaatatgat caagaaaatg caaagggtag aaaaaggggg
    17221 aatggatggt ggcagctctt actcagccaa agacattaca aaagaaatgc tgaaagaaga
    17281 gatcggaggc atcgctatct atcttgacct gctggcatca ttgatggata tcagtttgga
    17341 agaagccatt attgacactt tcaacagcaa gtcggataaa tatgggttca gccagagaat
    17401 agctacaggg cttaaccaga gaatcagtga agtactgtct gaaaaatgaa caatgaaatc
    17461 cccggccatt gtcatttttt gatcaaacag cggaagcaat tgactgacaa tagttaaaca
    17521 atgagaatgc ccggagaaaa ttaatctaca gagaaaggct atggcattat tcttgggctt
    17581 ttgggtatac aaaaatctaa aaatccattg tatgcaaaag tcagttttgt ccattatggt
    17641 ggtggcagct ttactgctgc tggcctcttg cagtaaagat gataatggcg gtcagctttc
    17701 ttaccagctc aaaacaaaga atccaaatgc taccgtaagc cgcaccagca gcggcagtgt
    17761 tacctggacc agtggttcag cctatgccga taaaattgaa tttgaagcag agaaggacga
    17821 tgacaatgaa gtggaatatg aatcaagagt ggctcgtcgc attgacctct ttgccccggt
    17881 cagccaactg ggcaatatct ccctgcctcc cggccgttac gaagaaatag aaattgaagt
    17941 agaccttgcc ccttcgataa ctgataccgc atttatttta cggggcagtt tcaccaacaa
    18001 tattggctat acagtgccgg tcgtatttta catcaacgaa ttactggagt tcaaagctga
    18061 agcagaagat atttcgatag gcagcgccgc agatataacg gcattgacta ctctggatct
    18121 ttctcagctc accaccggca tcacggaatc tatgctgagc agcgccacac ttaccaatgg
    18181 cgtacttgtt atatctgcaa ccagcaatgc cgctttatac agcgtcatac tttccaatat
    18241 caataacatt gatgacgtgg agctggatga ttaatcactt ttttgtttgc ccaaggtttc
    18301 ccgccccggt attgtccggg gcttttttat gtactttgtg ttatataaaa ttatgtctgc
    18361 ttatttcaac gatacattca ttgctgggga agaggtcaaa ctgcatgtgg ctgatctttc
    18421 cattcaacgg ggatatgccg tgtttgattt ttgccggaca gtaaacagaa aaccactttt
    18481 tataaatgat caccttgaac gcttcttcgc ttctgcagcg gccatgcacc tgccggtgaa
    18541 caaaacaaaa gaagaactga ttcacatcat tgggcaactc attcagcaat catccttgcc
    18601 ggaagcaggt atccgcatca tgctcaccgg tggttactca cccgacacct atacaccggc
    18661 ggtacccaac ctgctcatca cctgcaaccc ggtgaagaca gcaactgagg ctgattttga
    18721 aaagggggtt tcagttattt cttttgaaca ccagcgggaa ttgcctcata tcaaaacgat
    18781 caactacacg atggcggtgt ggctgcagcc tttggtaaaa gagaagaaag cagatgatgt
    18841 gttatatcat cataataaga tcatcaccga atttccgcgg agcaatgttt ttgtggtaac
    18901 aaaagataac cggctcctca cccctgccca caatatgctg aagggcatta cccgcaaaca
    18961 ggtattgtca ttggctgctg ctatcctgcc tgtggaagaa agagacattc accgggagga
    19021 agtatatgag gcagcggagc tatttctcac cagtaccagc aaaaaactta tgccggtggt
    19081 acgaatagat gatattgtta tcggggaagg aaaacccggt gtaataacaa gagagcttta
    19141 ccggagattt ttagaaaaag aaaaaaacca ataagattgg ccgtgtagca aaccttattg
    19201 gtttaatcaa tcattttaca gcgtactcat atcaatcaca aagcggtact ttacatcact
    19261 cttcagcaat cgttcatacg cttcattgat ctggtccata cggatgagtt caatatcaga
    19321 tacgatatta tgctcggcac agaaatccag catctcctgt gtttcaggta atccgccgat
    19381 gagtgagccg gcaatactct tgcggccaaa gatgaggttg aaagccggaa cagtggccgg
    19441 tgcggatggc gcacccacac ataccatcac cccgctggtg cgcaacaggc cgagatagaa
    19501 attataatca tgatcggctg aaaccgtatc gagaataaaa tcaaaataac ccatcactga
    19561 tttcacctgg gcttcatcac tggtcagtac aaatttgtgt gcgcccagcc gtttggcatc
    19621 tttctctttg gagggtgtat gactgagcat ggttacttca gctcccatag ctgccgccag
    19681 ctttaccccc atatgaccta aaccacccaa accgagtatg cctacccggg tgcccttgcc
    19741 cactttccag tggcgcaggg gtgaataagt ggtgatgccc gcacatagca gtggcgctgc
    19801 tgcatccagc ggcaggttgt cggcaatatg caatacaaaa tcttcatgca ccacgatgcc
    19861 cttgctgtaa ccgccatgtg tatggccgcc gcttatcttg tccggcgcnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nctcatgacc cggtacacag ggatagatcg cattgtgcca
    20101 ttcattgcgt acggtgtgca ggtcgctgtg gcaaacaccg cagtattgta tgtctatctg
    20161 tacatcatgc ggatggggtt ctcttctttc aatagttagc ggaacaagag gcgtggtggc
    20221 gctgtgggca ccgtaagctt tggtagcggg cataagtagt agttgagttg attaaagatg
    20281 ataaatgtaa tgctaatgca gcaaaccaaa gctgatggtt tttagtttta acggtgattt
    20341 catttaaccg gggcgatact cttttttatg tgacnnnnnn nnnnnnnnnn naagtttaat
    20401 ttttaaagcc cggttttgtg aattcattca acgggacaaa aaactgtact gaattttttt
    20461 agtattcccg ttttcttaaa attacaggta ggaaatctaa ataccttttt tattccagct
    20521 attgaaacaa atgcctaaaa aaattaatat tgcaataaaa gcgcag
//
LOCUS       DAORLS010000017        19866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1467_length_19866_cov_2.235627, whole genome shotgun sequence.
ACCESSION   DAORLS010000017 DAORLS010000000
VERSION     DAORLS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19866
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_19866_cov_2.235627"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /gene="alaS"
                     /locus_tag="PK977_02745"
     CDS             complement(<1..1298)
                     /gene="alaS"
                     /locus_tag="PK977_02745"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRF17051.1"
                     /translation="MTASEIRQAFLDFFQSKGHTIVPSAPIVVKDDPTLMFTNAGMNQ
                     FKDYFLGNKKSPYSRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYFK
                     NEAIEWSWELLTKVYKIPTDRLYATVFEGDEKEKLPASTIALAKWKEILPDPFTQIVF
                     GNKKDNFWEMGDTGPCGPCSEIHVDLRPEEERKKIPGHQLVNKDHPQVIEIWNNVFIQ
                     YNRKKDGSLEPLPATHVDTGMGLERLVRVLQGKTSNYDTDIFSGTIEATGNIVKKKYT
                     GGDDKESVAFRVIADHLRAISFTIADGQLPSNTGAGYVIRRILRRAVRYYYSYLDYKH
                     PLLTQLVPVLAKQFENVFPELQQQVDFVSKVVKEEEEAFLRTLDKGLKRIDDIIKTAS
                     SKIIAGKDAFELYDTFGFPIDLTRLIASENGLSVDEKGFEA"
     gene            1469..2440
                     /locus_tag="PK977_02750"
     CDS             1469..2440
                     /locus_tag="PK977_02750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF17052.1"
                     /translation="MKKIKYYYNTNTLRYEKLETPLRVRLLRIFGFIAAALVTAALIS
                     YVAFQFIGSPKEKILQEQNEDLRDNYRQLKDRIESLQQQMKELEKRDNDVYRAIFEAS
                     PIPDSARAKELETKLEIETVRRLKDNQLVGSILNTLNTLTSRIEAQKRSYDEVEELVK
                     NKEQLLARTPAIQPVSNKDLNRIASGFGYRIDPVYKTTKFHAGLDFSAPQGTPIYATA
                     DGTVTTAGNTGNGYGNHVIINHGFGYETLYGHMVRVKARNGQAVKRGEVIGWVGSTGK
                     STGPHCHYEVHKYGDKIDPIYFFYNDLTPEQFDMILKKAAASNQSLD"
     gene            2524..3072
                     /locus_tag="PK977_02755"
     CDS             2524..3072
                     /locus_tag="PK977_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRF17053.1"
                     /translation="MARPLTQITFDFAPTEETEQVPAARVAEKKPAETTVKIVNEAPP
                     KPQNPFSVTGKRGRKSLKEIDLKAELVQIPEDEILFKKQYYSIGEVATMFRENQSLIR
                     YWETEFDILQPRKNRKGDRFFRPVDIKNLVMIYDLLRRRKFTIEGAKDYLKKNKKAEE
                     KFAAIQSLQKIKNFFLELKASL"
     assembly_gap    3106..3214
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3215..3701
                     /locus_tag="PK977_02760"
     CDS             <3215..3701
                     /locus_tag="PK977_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF17054.1"
                     /translation="TTGVTLAQNQYQVLVERPNEKSLKGILSREAITGDTTFKWYADA
                     QKIYTPYAAAIEALQKNKDSVQLLVFMGTWCEDSHFVIPRLFKLTDAAGFSDNSISLI
                     GVDRNKKTLSHLCEALNVTNVPTIIVMKKGKELGRVVEYGKYGMFDKELGEIVNTAGS
                     N"
     gene            complement(3698..4786)
                     /locus_tag="PK977_02765"
     CDS             complement(3698..4786)
                     /locus_tag="PK977_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRF17055.1"
                     /translation="MNDFLEQIWWGNPVKNYFITGGIILFVWLLKKFISRYFAGLLFR
                     VVHSIWKDVDKKSFVNLVVKPLGFFLLILVSIVTLYKLNFPEELNPTIYKYTIKEIVH
                     CFGTIILIVSFIGLLLRIIDFIALILEKRANLTPDQTDNQLIVFFKDFFKVILVIIGV
                     MLVLRFAFDQNIGGLLTGLSIVGAAIALALRESIENLIASFIIFFDKPFVTGDLVKVQ
                     NITGTVERIGLRSTRIRTDQKTYVTVPNKQMVDSILDNLSLRTQRRADLKLELGLNTP
                     AAVTEQFIGGIEKILSRKEIESYNVHLSDITHTALVIVSDYYTAPVTIKEFNQLKQEV
                     NLQSLRLMEELKIEIAGASTDIRLQKQD"
     gene            complement(4828..5640)
                     /locus_tag="PK977_02770"
     CDS             complement(4828..5640)
                     /locus_tag="PK977_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17056.1"
                     /translation="MKKFACFFSLMVFCSLNIFSQGDTTFQLIRTLKGDIADFTVDNL
                     DNVYILTSRNQVKKFNANGDSVAVFNDVRKFGKATLVDVSNPLKVLLYYRDFATVVML
                     DRFLNVVNTIDLRKQNIFQAKAIGQSYDNKIWVYDEMENKLKKIDEDGKLLQETPDFR
                     LIFNQSPSPSKIFDENKYVYLYDSTKGINVFDYYGALKNNILITRWQNLKVTGKYIFG
                     SKADTLYRYEISNFVYDDWKMPGQIAKSRSFNFSSSRLYALKENSIEIYSIR"
     gene            complement(5637..6416)
                     /locus_tag="PK977_02775"
     CDS             complement(5637..6416)
                     /locus_tag="PK977_02775"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRF17057.1"
                     /translation="MLPNKKIYFLSDFHLGAPDHASSLEREKLLVQFLDKIREDAAEI
                     FLVGDMFDFWYEYRKVVPKGHVRLLGKLAELSDAGIQLHFFVGNHDMWMRDYFQQELN
                     MPVYFEPKEFVFNGKSFLVGHGDGLGPGDHGYKRLKKVFRNPVSKWLFGILPPVAGMS
                     LAHYMSRRSRAQTGSSEEVFLGEEKEWLIIYCKEVLTKKKIDFFVFGHRHLAIDYRLN
                     EQSRYINLGDWIRYFTYAVFDGENLQLKTYTGKEEKIIRKG"
     assembly_gap    6469..6508
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6509..6871)
                     /locus_tag="PK977_02780"
     CDS             complement(<6509..6871)
                     /locus_tag="PK977_02780"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRF17058.1"
                     /translation="MTSNGVYHLFVYGSLRSGFQSPVYEYISRFFELVGDAKAKGKMY
                     DLGEYPAAKPTDEECNIVGELYRIRNEKEFDWAIGQLDDYEGVNAEYDEVQQYYRETA
                     MVCYNNETIPAWIYWYSGD"
     gene            complement(6937..7572)
                     /locus_tag="PK977_02785"
     CDS             complement(6937..7572)
                     /locus_tag="PK977_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRF17059.1"
                     /translation="MNVSPLKENILKKIRKALSHSTPLPFPQSEGNQPLYAPLDQEPE
                     VEFAEQFTKLQGKFIYCINLQEFAFQLSSLIKKQDWKRVFCLEDKLIASASSQLADRL
                     VKTDLSNCDVSITGCEYLVARTGSIVMSAAQSSGRSASVYAPIHICIAYTSQIVYDIK
                     DALQSVKEKYGNNTPSLITFATGPSRTADIEKTLVVGVHGPKEVYLFLVEE"
     gene            complement(7582..>9262)
                     /gene="ftsH"
                     /locus_tag="PK977_02790"
     CDS             complement(7582..>9262)
                     /gene="ftsH"
                     /locus_tag="PK977_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRF17060.1"
                     /translation="PTVKEIPILSTSERDIFGGIIQFLLPVLLFVGLWILLMRKMGGG
                     AGGGGGPGGIFNIGKSKAQLFDKGTKVNITFADVAGLDEAKVEVMEIVDFLKSPKKYT
                     NLGGKIPKGALLIGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDL
                     FKQAREKAPCIIFIDEIDAIGRARGKNVMMSNDERESTLNQLLVEMDGFGTDVGIIVL
                     AATNRPDVLDSALLRPGRFDRQITIDKPDLVGREAIFKVHLKPIKISKSLDIHKLAEQ
                     TPGFAGADIANVCNEAALIAARKNKEAVDMQDFQDAVDRVIGGLEKKNKIISPEEKKI
                     IAFHEAGHAICGWFLEHAYPLLKVTIVPRGTAALGYAQYTPKEQYLYNTDQLNDQICM
                     TLGGRASEEIFFSKISTGAQNDLQQITRMAYAMVTVYGMNDKVGNISFYDPQQENTFT
                     KPYSDETAKLIDEEVRKLIDAAYDRTKKLLTDKKYEVETLATALLEREVLFQSDVEAL
                     IGKRPFEDKKHLAVEEAVVVEEKTEAKPAVETKQEESKDGLTGDIGLAPSI"
     assembly_gap    9263..9302
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9303..9703)
                     /locus_tag="PK977_02795"
     CDS             complement(<9303..9703)
                     /locus_tag="PK977_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF17061.1"
                     /translation="MSQDQNNPNRPPRFNGRPGGNEDPDQAPKKGPRFSIYWIYAIIF
                     AVLIGFQLFNPLSPNMAESNDIEFKSMVAAGDVSKAVIVKNRDKVKVYLTKQGQEKYA
                     DKLKKGVSGKISEDGPHMYFGVTSGDTFKKE"
     gene            complement(9774..10178)
                     /gene="rsfS"
                     /locus_tag="PK977_02800"
     CDS             complement(9774..10178)
                     /gene="rsfS"
                     /locus_tag="PK977_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRF17062.1"
                     /translation="MEQLSSLTNRKKKSAARLTKNSKIIKTIIAAIREKKGENIISLD
                     LRKINEAVADFFIICEAGNQPQIRAISDNVEDKVKELCGESPYHHEGHNNLQWVLIDY
                     VNVVVHVMLPETRKFYQLEEMWSDAAATGHEE"
     gene            10273..10878
                     /locus_tag="PK977_02805"
     CDS             10273..10878
                     /locus_tag="PK977_02805"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRF17063.1"
                     /translation="MPLPPSAILPESPFIELQSVDSTNNYARQLVHADLAQNKTGVFA
                     HEQLAGKGQRGKTWLTEKAGNIALSIIIKPQVIPLADQFQLSACAAVAAQVFFATYAG
                     DDTKIKWPNDLYWQDRKAGGILIESVVSSGESGISTWEWAIIGIGININQTHFPPHLP
                     NPVSLKQITGKTFDPVLLAKELHNIFLQQFDKLTTRGFSAI"
     assembly_gap    11017..11039
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11208..12005
                     /locus_tag="PK977_02810"
     CDS             11208..12005
                     /locus_tag="PK977_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17064.1"
                     /translation="MKHTPYSKALLWGLFNIFSLLLLLIACNKKTEKEVFRLPDEIII
                     SETVTRHPDLSNILGKKIKGEAIIFQLNSGEMIITIKSPNETEALNKFILQTKKTESY
                     KTELIKIKNAELVYLFDIVLLNDLDKNKLSSYYVNSATYKETYASLPALVRSSIETEI
                     PGYGLGYNKGSWPGLNENISLNNSVHTIRHLSQNIVDGGDGGGTTCTSGGVGSTSCSL
                     ANCFTQGCSVSCGAGYYACCYCDDVLPDLGPSCTCKENPPGGGGPPN"
     gene            complement(12078..12392)
                     /locus_tag="PK977_02815"
     CDS             complement(12078..12392)
                     /locus_tag="PK977_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRF17065.1"
                     /translation="MKVFIRENSWLAKLAAAKLNADQAAIVFGSTIHLHNTSRAEFLS
                     DKEWLCHELKHVEQYQQHGFAAFLVQYLFEWVKNGYYNNRFEKEARENEKKIDMNLYK
                     IL"
     gene            complement(12444..>12885)
                     /locus_tag="PK977_02820"
     CDS             complement(12444..>12885)
                     /locus_tag="PK977_02820"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRF17066.1"
                     /translation="FEDFSDRLSQVLQLIIARKMGKVSVQLNPTFVNRGYAVTYDKKT
                     MFALGGAIKLPLTGRINLVLDYFHSFRDQASKDAFKANENITFYDPLGVGFEVLTAGH
                     IFRLNFTNSTEILENRFIPRTITSWGEGQFRWGFTITRNFTLWR"
     assembly_gap    12886..12938
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12939..>13268)
                     /locus_tag="PK977_02825"
     CDS             complement(<12939..>13268)
                     /locus_tag="PK977_02825"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRF17067.1"
                     /translation="SEKAINANTTEVVPKGKMAFKVTHNFGDIAGDNGGARRFFGLDN
                     SADIRIAFTVGIANRLDLTAARAKGAGLHQQLYELGLKYQLLQQLENDPSHPIAVTLF
                     ANNVIAAN"
     assembly_gap    13269..13300
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13436..>13639)
                     /locus_tag="PK977_02830"
     CDS             complement(13436..>13639)
                     /locus_tag="PK977_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRF17068.1"
                     /translation="LPFRQIADTSHKILEKYGVWDQKQMFGHKYMGVLRTTFVIDEKG
                     IIRKIFLRPKNKAHAEEIIASMK"
     assembly_gap    13640..13691
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13692..13918)
                     /locus_tag="PK977_02835"
     CDS             complement(<13692..13918)
                     /locus_tag="PK977_02835"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRF17069.1"
                     /translation="MVILKEGDKAPAFTGADQDGKKVSLADYKGKKIVLYFYPEDDTP
                     TCTIQACNLRNNYGLLKKHGFEVIGVSPDDAA"
     gene            complement(13922..>14838)
                     /locus_tag="PK977_02840"
     CDS             complement(13922..>14838)
                     /locus_tag="PK977_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HRF17070.1"
                     /translation="VAKLPGDNGAAYKTINGDGVVTLADTMLHTVRIVVSDAKGLKSE
                     LNFMLQYIDSLASKANTTEYAMRFAPNKVNVISKPDFEMYLPKGALYDTITPYYYTVS
                     NKLPNTFSQAHQVANPAIPLHEDVVVRIKLNKPVPESMQDKLLIVRSDNKGRTIKKAV
                     WQEQWLSAAFGDFGTYQVIADTTAPQINNLGKGDTVNLSAVSRIIFTPTDNYGIKKFR
                     ATLNGKWIRFTNDKSRNWIYKFDERCPYGVHELIVTAEDLVGNTTTRTWWFRREPYTP
                     PPPKKKAIKKTSTKKKSSSDKKKSSKKK"
     assembly_gap    14839..14861
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14862..15753)
                     /locus_tag="PK977_02845"
     CDS             complement(<14862..15753)
                     /locus_tag="PK977_02845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF17071.1"
                     /translation="MTIKRSHWLYCLLVVVIFFNARKAASQLPVNYFRNPVGIPLDLS
                     ANFGELRPNHWHMGLDVRTNAKENLPIYAAAEGYIAHIGIRPQSFGRFIIINHPNGLS
                     TLYAHLNDFFPELEKFVTDKQYEKETWAIELDFTKEQFPVSKGAFIAYSGNTGGSQGP
                     HLHFEIFETKTGKRLNPLLFNFNISDNVPPAITKLAVYDRSRSVFDQKPLFYNLKNTD
                     SGYIIPKMPVMKTGLNRLSFAIQAYDKMSSSGSPDGIFSAQLYFDDIPQVKFVLDSID
                     YEETAYINSQTDHMHRYTGGA"
     gene            15825..16535
                     /locus_tag="PK977_02850"
     CDS             15825..16535
                     /locus_tag="PK977_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17072.1"
                     /translation="MTNKKIASFLLIASFLFYTDTFSQNCKVEKEELKGSYTGDCKKG
                     KANGKGKATGIDSYEGDFVTGLPHGKGSYTWKNGDVYTGEFNKGMRDGEGKMIIKRPG
                     NTDSLIEGYWQNDKYTGLKEKPWRLIFKSKLVTETEVEYKDNHFNKITFVITNTSGGS
                     KLVEGDEMPRLKVDEVIPQRGNFGRLFVNDIHAKKTESLIEDINFPFRFRAIIGTEEV
                     EMEFVRPGNYIVTLRIND"
     assembly_gap    16706..16724
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16725..17190
                     /locus_tag="PK977_02855"
     CDS             <16725..17190
                     /locus_tag="PK977_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17073.1"
                     /translation="GYWYGIIKDGSDYFGIEALEITPKSDSVYSITRYGKAIKGDIIM
                     PDTAYYTGFTSWIGNQRFMNVEGLVKIEEVTGKKKREVKIHRVFYASALDQRKDTLEV
                     RTITEDFSTRKYFGDPDHFRLLVTEMLKLQQNIYDAQYSMQYRKIPKPAKLF"
     gene            17306..>19866
                     /gene="carB"
                     /locus_tag="PK977_02860"
     CDS             17306..>19866
                     /gene="carB"
                     /locus_tag="PK977_02860"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRF17074.1"
                     /translation="MPKDNSIQSVLIIGSGPIIIGQACEFDYSGTQAARSLREEGVKV
                     ILINSNPATIMTDPMMADRVYLLPLTVESIEQILQENQIDAVLPTMGGQTALNLAKEA
                     EELGLWKQYNVRLIGVDIKAIEKAEDREKFRQWMIQLGVNVAQAKTANSFLEGKEFAQ
                     EIGFPLVIRPSFTLGGTGGGFVHGKDDLDEALNRGLQASPIHEVLVEKAVLGWKEFEL
                     ELLRDAADNVCIICTVENFDPMGVHTGDSITVAPAMTLSDTAMQLMRNTAIRMMRDLG
                     NFAGGCNVQFALNPDTEEIIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LENQITKTTSAYFEPALDYVIVKIPRWNFDKFKGADDTLGLQMKSVGEVMGIGRSFTE
                     AVQKACQSLENNAVGLGYYGKSLMHADELLEYIKTPKWDRIFRIKDALMAGISVSSIS
                     KATNGIDRWFLYEIQKLCNIEKEIAKYGLEDLPIELMREAKVNGFSDEQISRITGHGD
                     EEDVYKKRKAAGITRVYKMVDTCSAEFEAKTPYFYSTFESGNHNESKVSDRKKIIVLG
                     SGPNRIGQGIEFDYCCVHGLLAIKECGYEAIMVNCNPETVSTDFDMADKLYFEPVYWE
                     HLWEIIEHEKPFGVIVQLGGQTALKLAEKLHEKGINIIGTSFNSLDIAEDRGRFSDML
                     KELDIPYPNYGTAYTVDEAIEVANKVGYPVLVRPSYVLGGQRMRIVLNDEEVEKAVVS
                     LLKHIPGNKILIDHFLDRCQEAEIDAICDGEKFHVMGIMEHIEPAGIHSGDSNAVLPY
                     FNLSPLEVQDMVDYAKKIALALNVKGLINIQFAIKDGKVYVIEANPRASRTTPFIA"
BASE COUNT         5722 a         4219 c         3943 g         5591 t
ORIGIN      
        1 gcttcaaagc ctttctcatc aacggataat ccgttttcgg aagcgatcaa ccttgtaagg
       61 tcaatcggaa aaccaaatgt gtcatataat tcaaaagcat ccttacccgc tattattttt
      121 gaagaagcag ttttgatgat atcatcaatt cgttttaatc ccttatccag cgttcttaaa
      181 aatgcctctt cctcctcttt caccacctta cttacaaaat caacctgctg ttgcaattca
      241 ggaaatacgt tttcaaattg ttttgccaaa acaggcacca actgtgtcag caaaggatgt
      301 ttgtaatcca ggtaagaata ataatacctt accgcccttc tcaaaatcct ccttatcaca
      361 taaccggcac cggtattgga aggtaattgc ccgtcggcaa tagtaaagct gatggcacga
      421 aggtggtcag caatcacccg gaaggctaca ctttccttat catcgccacc tgtatatttt
      481 ttcttaacga tgttgccggt agcctctatc gttccggaga aaatatccgt atcatagtta
      541 gaagttttgc cctgcaatac ccttactaat ctttccagcc ccatacccgt atccacatgg
      601 gtggcaggta aaggctccag gctcccatct tttttgcggt tgtattgaat aaatacatta
      661 ttccatattt caataacctg gggatggtct ttatttacca gttggtgtcc gggaattttt
      721 tttcgctcct cttcaggtcg caggtccaca tgtatctcgc tgcagggacc gcaagggccg
      781 gtatcgccca tctcccaaaa attatctttc ttattaccaa aaacaatttg cgtaaacgga
      841 tcagggagaa tttctttcca cttagccaat gcaatagtag aagccggtaa tttttctttt
      901 tcatctcctt caaacacagt agcgtaaaga cggtctgttg gtatcttgta aactttggtc
      961 agcaattccc aactccattc aattgcctca tttttgaaat aatcgccaaa gctccagttg
     1021 cccagcatct cgaacatagt gtgatgatag gtatctactc ctacttcttc cagatcattg
     1081 tgcttgccgc taacccttaa acatttttgg gtatcggcca cccttgaata aggtgatttt
     1141 ttatttccca aaaaataatc cttgaactgg ttcattcccg cattggtgaa catgagagta
     1201 ggatcatcct ttaccactat cggggcagat ggaacaattg tatgtccttt cgattgaaaa
     1261 aagtccagga atgcttgtcg tatttcactt gctgtcatat cgtacttttt tggcgaaatc
     1321 gctgatttct ttaccactca ccgtttctgc ctttgttctg ggttgatttt tgcggtatat
     1381 ggggctatat ttgtcgtccc ccgcaaggcc gcaaaggtaa agattcagta cggtatttat
     1441 aggctgtagt ggggtgggtt gcaggctcat gaaaaaaatc aagtactatt ataataccaa
     1501 tacgctccgg tatgaaaaac tggaaactcc ccttcgggtg agattgctgc gtatatttgg
     1561 attcatagct gccgccctgg ttacagccgc cctgatttct tacgttgctt tccagtttat
     1621 cggctctcca aaagaaaaga tcctgcagga acaaaatgaa gacctccggg ataattaccg
     1681 gcaacttaaa gacaggattg aatcgcttca gcagcaaatg aaagagctgg agaaaagaga
     1741 taatgatgta tacagggcta tcttcgaagc cagcccaata cctgatagtg cacgggcaaa
     1801 ggaactggaa accaaactgg aaattgaaac agtaagacga ctaaaagata accaattggt
     1861 aggctctatc ctgaatacac tgaatacact caccagtcgt atagaggcac aaaaaagatc
     1921 ctacgatgaa gtagaggaac tggtaaaaaa caaggaacaa ctgctggcca gaacaccagc
     1981 cattcaacca gtgagcaata aagaccttaa ccgtattgcc tctggttttg gttatcgaat
     2041 agaccccgtt tacaaaacca ctaaattcca cgcagggcta gatttttccg ccccgcaggg
     2101 aacacccatt tatgccaccg ctgacggtac cgtaaccact gcaggaaata ccggtaatgg
     2161 ttatggcaac catgtgatca ttaaccatgg atttggttac gaaactttat atggccacat
     2221 ggtaagagta aaagccagaa acggccaggc ggtaaaaagg ggtgaagtaa tcggctgggt
     2281 ggggagtact ggaaaatcaa ccggcccgca ctgtcattac gaagtacata aatacggtga
     2341 taagatcgat cccatatatt tcttctataa tgacctcacc ccggagcagt ttgatatgat
     2401 actcaagaaa gctgccgcca gtaaccaaag tcttgactaa ggtgatacaa ctgatttttc
     2461 cacaagtata gaaggtgacc attatccgcc gctggctttg ttatctttac attaattatc
     2521 actatggcaa ggcccctaac acaaataacc tttgattttg ctccgacaga agaaactgaa
     2581 caggttccag ctgcccgggt tgcagaaaag aagccggcag agactacagt gaaaatcgtt
     2641 aacgaagccc cgccaaaacc gcaaaaccct tttagtgtta caggaaaaag aggtagaaaa
     2701 tcgcttaagg aaattgatct caaagctgag ctggttcaga ttccggaaga tgagatacta
     2761 ttcaaaaaac aatattattc cattggggaa gtggccacta tgttcaggga gaatcaatcg
     2821 ctgatccgat actgggaaac agaatttgac attttacaac cccggaaaaa cagaaaaggc
     2881 gatcgttttt tccgccccgt tgatatcaag aacctggtaa tgatctatga cctgcttcgt
     2941 cgtcgcaaat tcactattga aggggcaaag gattatttga aaaaaaataa aaaagcagaa
     3001 gaaaagtttg cagccatcca gtctttacaa aaaatcaaga attttttcct ggaacttaaa
     3061 gccagtctgt gactaaaaaa gtcacctttt tgcaacttta acatcnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacaac aggagttacc cttgcccaaa
     3241 accaatacca ggtattagtg gaaaggccca atgaaaaatc gttgaaaggg attctgtccc
     3301 gggaagctat aactggggat actacattta aatggtatgc cgatgcacaa aaaatctata
     3361 ctccttacgc tgccgcaata gaagctttgc aaaagaataa agattctgta caactgctgg
     3421 tgtttatggg gacctggtgc gaggattctc attttgtaat accccggttg tttaaattaa
     3481 cagatgctgc aggcttcagc gataacagta tttcattaat tggtgttgac cggaataaaa
     3541 aaaccttgag tcacctttgc gaagcactta acgttacaaa cgttcctacc atcattgtta
     3601 tgaaaaaagg gaaagaactg ggccgtgtag tagaatatgg caaatacggg atgtttgaca
     3661 aggaattggg ggagatcgtc aatacggctg gaagtaatta atcctgcttt tgcaacctga
     3721 tatcggtgct ggcgcctgca atttcaattt tcaattcttc cattagccgc aggctttgca
     3781 ggttcacctc ctgttttaac tggttaaact ccttaatggt aacgggagct gtatagtaat
     3841 ctgaaacaat gacaagggct gtatgtgtta tatctgacag gtgaacatta tagctttcaa
     3901 tctcctttcg gctaagaatc ttttctatac cgccgatgaa ttgttctgtt actgcagcag
     3961 gagtatttaa acccagttcc agcttaaggt cggctcttcg ctgagtacgt aaagacaaat
     4021 tatccagaat gctgtccacc atttgcttat tgggaactgt aacatatgtt ttctgatctg
     4081 ttctgattct tgtgcttctt aacccgattc tttccactgt tccggtaatg ttttgcactt
     4141 taaccaggtc gcctgtcaca aatggtttat caaaaaagat tataaaagag gctatcaggt
     4201 tctcgatgct ttctcttaat gccagggcaa tagctgcacc tacgatactt aaacccgtta
     4261 acagtccgcc gatattctga tcaaaagcaa aacgaagcac cagcatgacg ccgatgatca
     4321 ctaaaatgac tttaaaaaag tctttaaaga aaacgataag ctggttatct gtctgatcag
     4381 gtgtgaggtt agcccttttt tccagtatca gggcaatgaa atcaatgatg cggagcagca
     4441 acccgataaa agaaacgatg agaataatag ttccaaaaca atgaacaatc tccttgatcg
     4501 tatacttata gatagtagga ttgagttcct ccggaaaatt cagcttgtat aatgtgacaa
     4561 tagaaacaag gatcaggaga aaaaaaccaa gtggctttac taccagattc acaaaagatt
     4621 tcttatccac atctttccag atgctgtgta ccaccctgaa cagcaggccg gcaaaatagc
     4681 gggaaatgaa tttcttcagc aaccaaacaa aaaggatgat gccgccggta ataaaatagt
     4741 tttttacagg gttgccccac catatctgtt ccaggaaatc attcatacca caaaaatatt
     4801 gattagcgcc gaagctgtgt acaacagcta tcgtatggaa taaatctcaa tactgttttc
     4861 ctttaacgca taaagccggc tcgaagaaaa attgaacgac ctgctcttag ctatttgccc
     4921 aggcattttc cagtcgtcat aaacaaaatt gctgatctca taccggtata aagtatcagc
     4981 ctttgagcca aaaatgtatt tacccgtcac tttaaggttc tgccaccggg tgatcaggat
     5041 attattcttt aaagcaccgt aataatcaaa tacattaatc cctttcgttg aatcatagag
     5101 gtagacatat ttattttcat caaatatttt tgaaggagat ggcgactgat taaagatgag
     5161 cctgaaatcc ggcgtttcct gcagcagttt cccatcttcg tcaatcttct tcaatttatt
     5221 ttccatttca tcgtaaaccc aaattttgtt atcgtacgac tggccaatgg ctttggcctg
     5281 gaaaatattt tgtttgcgga gatcaatagt attgaccaca tttaaaaagc ggtcgagcat
     5341 tactacagta gcaaaatccc tgtaatacaa cagtaccttc aaggggtttg acacatccac
     5401 caaagtagct tttccgaatt ttcttacatc attaaaaacg gctaccgaat cgccattcgc
     5461 attgaatttt tttacctggt tacggctggt caggatataa acgttatcga ggttgtcaac
     5521 ggtaaaatcg gctatatctc ctttcagtgt ccgtattaac tgaaatgtgg tatccccctg
     5581 actgaaaata ttcagcgagc agaagaccat tagtgaaaaa aaacaggcaa attttttcat
     5641 ccttttcgga ttattttttc ttcttttccg gtataagttt ttagctgcag gttttcaccg
     5701 tcaaatactg cgtaagtaaa gtaccggatc caatcaccga gattaatgta tcggctctgt
     5761 tcattcagac gataatctat ggctaaatgt ctatgcccaa atacaaaaaa gtcgattttc
     5821 tttttggtca gtacttcttt acaataaatg atcagccatt ctttctcttc acccaggaac
     5881 acttcttccg atgaacccgt ctgtgcccgg cttctgcggc tcatgtaatg ggcaagactc
     5941 atgccggcta ccgggggaag aatcccaaat aaccatttac taaccgggtt tctaaagact
     6001 ttcttaagcc gtttataacc atgatcgccg ggaccaagac catcaccatg accaaccagg
     6061 aatgattttc cgttaaatac aaactctttg ggttcgaagt aaacaggcat gttcagttcc
     6121 tgttgaaaat agtccctcat ccacatatca tgattgccca caaaaaaatg aagctgaatt
     6181 cctgcatcag atagctctgc cagtttgcct aacagcctta catggccctt cggaacgacc
     6241 tttcggtact cgtaccagaa atcaaacata tctcctacga gaaatatttc tgcagcatct
     6301 tctcgtattt tatcaaggaa ttgaacgagc aatttttccc tttcgaggct ggaggcatga
     6361 tccggcgcac caaggtgaaa gtcagaaaga aagtatattt tcttatttgg aagcaagtat
     6421 tacacagtgt ttattgcttg tccttcaggt attgtaacat atcgccggnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cgccgctata ccaatatatc caggcaggaa
     6541 tcgtttcatt attataacag accatggcag tttcccggta gtattgctgc acttcatcat
     6601 actctgcatt taccccttca taatcatcca actgcccgat agcccagtca aattcttttt
     6661 cgtttctgat gcggtataat tcacctacaa tattgcactc ctcatcggtt ggtttggctg
     6721 cgggatattc acccaggtca tacatctttc ccttagcctt agcatcacca accaattcaa
     6781 aaaaccggct tatatactca taaacagggc tttgaaaacc gctgcgcaaa gagccatata
     6841 caaaaagatg atatacacca ttactcgtca tgatataaag ataggccaac cgttaaactc
     6901 aggcaagaaa tagcaacgaa agagtgatga ttttcatcat tcctctacca ggaaaagata
     6961 cacctctttg ggtccgtgta ccccgaccac taacgtcttt tcaatgtctg ctgtgcggct
     7021 ggggcctgtg gcaaaagtta tcagagaagg agtgttgttg ccatattttt ctttaacaga
     7081 ttgcagggca tcttttatat cataaacaat ctggctggta taagctatac aaatgtgaat
     7141 gggcgcatac acactggcgc tgcgtccgct gctttgtgct gcactcatta caatacttcc
     7201 tgttctggcc accaggtact cgcaaccagt aatggaaaca tcgcaatttg aaagatcagt
     7261 ctttactaat ctgtcagcca actgggaaga agcagatgca attaatttat cctcgaggca
     7321 aaatactctt ttccaatctt gtttttttat aagactgctt agttgaaaag caaattcctg
     7381 caggttaata cagtaaatga acttaccctg caacttagta aactgctcag caaactccac
     7441 ttctggctcc tgatccaaag gagcatatag aggctggtta ccttcacttt gcggaaaagg
     7501 taaaggcgtt gaatgactca acgccttacg tatttttttc aggatgtttt cttttaaggg
     7561 agaaacattc atagaaagtt attaaataga tggagctaat ccgatatcac ctgttaagcc
     7621 gtcttttgat tcctcctgct ttgtttcaac agccggcttt gcttccgttt tttcttccac
     7681 taccacagct tcttccacag ccaaatgctt tttatcttca aaaggacgtt tgccgatcag
     7741 ggcttccaca tcactctgga acagcacttc tctctccagt aatgccgtag ccagagtttc
     7801 tacttcatac ttcttatctg tcaatagctt tttagttctg tcgtaagctg catcaatcag
     7861 cttccttact tcttcatcaa tgagcttggc cgtttcatca gaataaggct tagtgaaagt
     7921 attttcctgc tgaggatcgt agaagcttat gttccctact ttatcgttca taccgtaaac
     7981 cgtcaccata gcataagcca tacgggttat ttgctgcaaa tcattttgtg caccggtaga
     8041 aattttactg aagaaaatct cttcgctggc cctgccgccc agtgtcatac aaatctggtc
     8101 attgagctga tcagtattgt acaggtactg ctctttggga gtgtattgcg catatcccaa
     8161 tgctgctgtg cccctgggaa cgatcgttac ttttaataaa ggataagcat gttccaggaa
     8221 ccagccacag atagcatgac ctgcttcgtg gaaagcgatg atctttttct cctcgggaga
     8281 tatgatctta tttttctttt ccaatcctcc gatcacacgg tcaaccgcat cctggaaatc
     8341 ctgcatatcc actgcctctt tattcttacg ggcagcaatc agggctgctt cgttacaaac
     8401 atttgcgata tctgcgccgg caaaaccagg ggtttgctca gccagtttat gaatatcaag
     8461 tgacttagaa attttaatcg gcttcaggtg aactttgaag atggcttcac gaccaaccag
     8521 gtcaggttta tcaattgtaa tctgcctgtc gaatcggccc gggcggagca atgcagagtc
     8581 caatacatcc ggacggttgg tagccgccag tacaataatg cccacatccg taccgaatcc
     8641 gtccatctcc accaacaatt ggttcagggt gctttctctt tcatcattac tcatcatcac
     8701 attcttaccc cttgcacggc caatggcatc aatttcatcg ataaagatga tacagggtgc
     8761 tttttccctc gcctgtttaa ataaatctct aacacggctg gcgccaacgc caacaaacat
     8821 ttccacaaaa tcggaaccac taaggctaaa gaaaggtacc tgtgcttctc cggccaccgc
     8881 ttttgccaat aatgttttac cggtaccggg aggaccaatc agtaaagcac ctttgggtat
     8941 tttaccgcca aggttggtgt actttttagg acttttcaaa aaatcaacaa tctccatcac
     9001 ttccactttc gcttcatcca ggccggcaac atctgcaaaa gtgatattga ccttcgttcc
     9061 tttatcgaaa agctgggctt ttgatttgcc aatattgaat atacctccag gaccgccacc
     9121 accgcctgct ccgccaccca tcttgcgcat tagcaggatc cagaggccta cgaataataa
     9181 aacggggagc aggaactgga taatacctcc aaaaatgtct ctttcagacg tagacagaat
     9241 aggaatttcc tttacagtag gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnatttcctt tttgaaagta tctccactag tgacaccgaa atacatatga ggaccatctt
     9361 cacttatttt tccagatact cccttcttta atttatccgc atatttttcc tgcccttgtt
     9421 ttgtgagata tactttgacc ttatccctgt tcttgacaat aacagcctta ctcacatcgc
     9481 cggctgctac cattgactta aactcgatat cattgctctc cgccatattg ggcgacagcg
     9541 ggttgaacaa ctggaagccg atcaatacag cgaatatgat ggcataaatc caataaatac
     9601 taaaacgagg gccctttttg ggagcctggt ctgggtcttc atttccaccg ggacggccat
     9661 taaacctggg aggtctgttt ggattatttt gatcttgtga catattttat tttctgatcc
     9721 tgctatttaa tgataattaa gataatttgt tgacagcaat tccatattta acattactct
     9781 tcgtgtccgg tagctgcagc atcgctccac atttcttcca gctgataaaa tttcctggtt
     9841 tcgggcagca tcacatgcac caccacattc acatagtcaa tcagcaccca ttgcaggttg
     9901 ttgtgtccct catggtgata tgggctttct ccacaaagtt cctttacctt atcctccaca
     9961 ttatcagaaa tagcccttat ttggggctgg ttacccgcct cgcaaatgat gaaaaaatca
    10021 gcaactgctt catttatctt ccggaggtcc agggatatga tattttcacc ctttttctcc
    10081 ctgatagctg caatgatggt tttgatgatt tttgaattct tcgttaaccg tgctgcactt
    10141 ttctttttgc ggttggtcag tgatgaaagt tgttccaata atctagttct ttttgtttgt
    10201 gaagtttttt ggcaaccgga aaccattaac taatacccgg aaaccagtta ctttgtcaaa
    10261 aatagcaatt aattgccact ccctccttct gcaatactac cggaatcacc attcattgag
    10321 ttgcaatcgg ttgatagcac caacaactat gcccgtcaac tggttcatgc ggatttggca
    10381 cagaacaaaa cgggggtttt tgcccatgaa cagctggcag gcaaaggtca acggggcaaa
    10441 acctggttaa ctgaaaaggc tggtaatatt gccctcagca tcattattaa gcctcaagtc
    10501 ataccacttg ctgaccagtt tcagctaagt gcctgcgctg cggtagctgc ccaggttttc
    10561 tttgccacct atgccggaga tgacaccaaa ataaagtggc ctaacgacct gtattggcag
    10621 gacagaaagg caggcggaat attaattgag tcagtagtca gcagcgggga atctggaatc
    10681 agtacctggg aatgggctat cattggtatc ggtatcaata ttaatcaaac ccactttccg
    10741 ccccatttgc ccaatccggt ttcgctgaaa cagataaccg gaaaaacctt tgatcctgtt
    10801 ttattggcta aagagctgca caacattttc cttcaacaat ttgataaatt gactacccgg
    10861 ggcttttcag caatctagga acattaccaa gccaatcttt ataaaaaaaa tgaaagagta
    10921 cggctaaaaa aagacaaccg gtcttttgaa gcaacgatta gagaggtaag taaatcgggt
    10981 aaacttattg ccgaacacgg aatagtagaa gagtttnnnn nnnnnnnnnn nnnnnnnnnt
    11041 tgagtggata caacttcata gctgatttca gcttcaatta atcccgatat ttttattctt
    11101 gattttcaat gtataagagc aacagaattt tttttttgtt aagttcaaaa aaaaaaaaaa
    11161 ataattttat gtgtgacttc taattgtttt accaaaaatt attagacatg aaacacacac
    11221 cttattctaa agcccttcta tggggcttgt tcaatatctt ttcattgctc ctgctcctta
    11281 ttgcatgtaa taaaaaaaca gaaaaggaag ttttcagatt acctgacgaa atcattattt
    11341 ccgaaactgt aacgagacac ccggatctta gtaatattct tggaaaaaaa ataaaaggag
    11401 aggcaataat atttcagctg aacagcggcg aaatgatcat tactattaaa tctccaaacg
    11461 aaacagaagc ccttaacaag tttatccttc aaactaaaaa aaccgaatca tataaaacag
    11521 aattaataaa aataaaaaat gcagaactgg tttatctttt tgatatagtt ctattaaatg
    11581 atcttgataa aaataaactt tcttcatact atgtaaattc tgccacatat aaagaaactt
    11641 acgcttcact tccagcctta gtcagatcat caatagaaac cgaaattccg ggatatggcc
    11701 tcggttataa taaaggaagt tggcccggat taaatgaaaa catttccttg aataattcgg
    11761 ttcatactat tcgtcatctt tctcaaaata tagttgatgg aggcgatggt ggcggcacaa
    11821 catgtacatc tgggggagtg ggctctacta gttgctcctt agcaaactgt tttactcaag
    11881 gatgttcagt tagttgtggt gcaggatatt atgcttgctg ttattgtgac gatgtgcttc
    11941 cggatctagg tccttcttgt acatgtaagg aaaatcctcc cggagggggc ggtcctccca
    12001 attaataagg tattaatagg ttaagttttc agatagaaaa agaggctgtt ttatcaaata
    12061 gcctcttttt ttaagagtta taatatttta tagagattca tatctatctt tttttcattc
    12121 tcccttgctt ctttctcaaa ccggttatta taatacccgt tcttcaccca ttcaaacaga
    12181 tattggacaa gaaaggctgc aaagccatgt tgctgatatt gctccacatg cttcagctca
    12241 tggcatagcc attccttatc tgacagaaac tcagcacggc tggtattgtg taaatgaatg
    12301 gtactgccaa aaacaatggc agcctgatca gcattcagtt ttgcagctgc cagtttcgcc
    12361 agccatgagt tctcccgaat aaaaactttc atgcaactat gataaatgaa taagcccaat
    12421 agttgcacta gcatttacct tccctaacgc cacaaagtga aatttcgggt aatagtaaat
    12481 ccccatcgaa actggccttc accccaggaa gtaatagtgc ggggaatgaa gcggttttcc
    12541 agtatttccg tcgagttggt aaaatttagc ctgaaaatat gcccggccgt caacacttca
    12601 aaacccactc ccagcggatc ataaaatgta atattctcgt tggctttaaa agcatcttta
    12661 ctggcctgat cacgaaaaga atgaaaataa tcgagtacta aatttatcct tccagtcaat
    12721 ggtagtttta tcgccccgcc cagggcaaac atggtttttt tatcatacgt aactgcatag
    12781 ccacgattta caaaagtggg atttagctgt acactcactt ttcccatctt tctggcaatg
    12841 atcaattgca gtacctggct tagcctgtcc gaaaaatctt caaaannnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tagccgcaat aacattattg
    12961 gcaaaaagag taactgcaat aggatgagaa ggatcatttt ccagttgttg cagcaactgg
    13021 tatttcaaac ccagttcata caattgctga tgcaaaccgg cacctttggc cctggcagcg
    13081 gtgaggtcaa gccggttggc tataccgact gtaaacgcaa ttcttatgtc tgcagaattg
    13141 tccagtccaa aaaaacgcct ggccccgccg ttgtctccgg caatatcacc aaaattatgt
    13201 gtcaccttaa acgccatctt gccttttggc actacttctg ttgtgttggc attgatggct
    13261 ttttcagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accggctttt tgtcttgcga
    13321 aaaaatagtg aacgggaaaa aaaataatgc tattgtcagt gatttgatca taaatgcagt
    13381 tttttcttgc tgcatttatc ggcaatacct gttgaaacgt tgcctggtaa tatacctatt
    13441 tcatggaggc tataatttcc tccgcatgag ctttgttttt gggacggagg aatattttac
    13501 gaatgatgcc cttctcatca ataacaaaag tggtacgcaa cacccccatg tatttgtgac
    13561 caaacatttg tttctgatcc catacgccgt atttctccag tattttgtga gaagtatcgg
    13621 cgatttgtct aaaaggaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn ngccgcatca tcagggctga cacctattac ttcaaatcca tgcttcttca
    13741 ataatccata attgttacgc aggttgcagg cttgtatggt gcaggtaggc gtatcatctt
    13801 cggggtagaa atacaaaaca atctttttac ccttataatc agccagcgaa actttttttc
    13861 catcctggtc cgcaccggta aatgcgggcg ccttatcccc ttccttcagt attaccataa
    13921 cttatttctt tttggagctt ttctttttat cagaagatga tttctttttg gtgctggttt
    13981 tcttgatagc cttcttcttg ggtggtggcg gtgtataggg ttcccgcctg aaccaccatg
    14041 ttcttgtagt ggtatttcca acaaggtcct ctgctgttac aatcagctca tgtacgccat
    14101 aggggcatcg ctcatcaaat ttgtatatcc agttccttga tttgtcgttg gtaaatctta
    14161 tccatttgcc gtttagtgta gccctgaatt ttttaatccc ataattatcg gtgggagtaa
    14221 atatgatcct gctgactgca ctcagattta cagtatctcc tttaccaagg ttattgatct
    14281 gcggagctgt agtatctgct ataacctggt aggttccaaa atcgccgaag gcagcagata
    14341 accattgctc ctgccaaact gcttttttta ttgtgcggcc tttattatcc gatcttacaa
    14401 tcagcagctt gtcctgcata gactcaggta ctggcttatt tagttttatt ctcaccacca
    14461 catcctcatg caagggtatg gcaggattgg ccacctggtg tgcctgggaa aaagtattgg
    14521 gcaacttatt gcttacagta taataatatg gagttatcgt atcatacaaa gctcctttgg
    14581 gtaagtacat ttcaaaatcc ggtttgctaa tcacatttac tttattaggg gcaaatcgca
    14641 ttgcatactc tgtggtgttt gcttttgatg ccaggctgtc aatatactgg agcataaagt
    14701 tgagttcaga tttcagccct ttagcatcac tcactactat gcgaaccgta tgaagcatgg
    14761 tatcagctaa agtaactaca ccatcaccgt tgatcgtttt ataagcagcc ccgttatcgc
    14821 ctggtagttt agctacatnn nnnnnnnnnn nnnnnnnnnn naggccccgc ctgtgtaccg
    14881 gtgcatatgg tctgtttgcg agttgatata agcggtttcc tcataatcaa tactgtccag
    14941 tacaaatttt acctgcggaa tatcatcaaa gtaaagttgt gctgaaaaaa ttccgtccgg
    15001 gctgccactg ctgctcattt tatcataagc ctgtatggca aaactcagcc ggttcaatcc
    15061 cgttttcata accggcattt taggaataat atagccgctg tcggtatttt tcaggttata
    15121 gaataacggc ttttgatcaa atactgagcg gctcctgtcg tatacagcca gcttggttat
    15181 tgccggaggt acattgtcac taatattaaa attaaatagc aaggggttta accttttgcc
    15241 ggtttttgtc tcaaatattt caaagtgtaa atgcggtccc tgcgagccgc ctgtattacc
    15301 gctataagca ataaatgctc cctttgaaac aggaaattgt tctttagtaa agtcaagttc
    15361 aatagcccag gtttctttct cgtattgttt atctgtaaca aatttttcca gctcaggaaa
    15421 aaaatcattc aaatgggcat acaaagtaga aaggccgttg ggatgattga tgattatgaa
    15481 ccggccaaaa ctctgcggcc ggataccaat atgggcaata tatccttcag ctgcagcata
    15541 gataggcagg ttttcttttg cattggttct cacatcaagt cccatatgcc agtggtttgg
    15601 tcttagttca ccaaaattgg cagaaaggtc taaagggatg cctacaggat tcctgaaata
    15661 attaaccggt agctgcgaag cagcctttct tgcattaaaa aaaattacca ccactaataa
    15721 acagtacaac caatggcttc tttttattgt catgtttaca aattaacaac atgtcggtta
    15781 gttttctgca ttaacaagat aactttgaac atattttaat gtttatgacg aataaaaaaa
    15841 tcgcttcttt cttacttatt gcttcctttt tattttatac agatacattt tcccaaaatt
    15901 gtaaagtaga aaaagaagaa ctgaagggca gctataccgg ggactgcaaa aagggaaagg
    15961 caaatggcaa gggcaaagca acagggattg acagctacga aggtgatttt gttacggggc
    16021 ttccgcatgg caaaggcagt tatacatgga aaaacggcga tgtgtacacc ggtgaattca
    16081 ataaaggaat gagagatggc gaaggtaaaa tgatcataaa acgaccgggg aatactgaca
    16141 gtcttattga aggatactgg caaaatgata aatatacagg gttaaaagaa aagccatggc
    16201 ggttgatttt caaaagcaaa ctagtaacag aaacggaagt agaatacaaa gacaatcatt
    16261 tcaacaaaat cacttttgtg atcactaata cttccggcgg ctcaaaactg gttgagggcg
    16321 atgaaatgcc ccggttaaaa gtggatgagg tgataccaca acgaggaaat ttcggacgat
    16381 tattcgtaaa cgatatacat gccaagaaaa cagaatcgct gatcgaggac attaattttc
    16441 cttttcgttt cagggccatc atcggaacgg aagaagtgga gatggaattt gtaagacccg
    16501 gtaattacat agtgaccttg cggatcaatg attaaaaagg taattatacc gttttgtggt
    16561 ataggaagcg tacttgatta gttgtatctt ttcgcaccaa aattttaagg ttgaaaaaac
    16621 tttgctggct gttgccgtta tcattcatct tatgtgcctg tccctttgag tcggatgtgg
    16681 cgctggaaaa gaaaccggtt gaaccnnnnn nnnnnnnnnn nnnngggata ttggtatggc
    16741 atcatcaaag acggcagtga ttattttgga atcgaagcac tggagataac gcccaaatcg
    16801 gactcagtat attccataac ccgttatggc aaggctatca agggagacat tatcatgcct
    16861 gacacagctt actatactgg ctttacctca tggataggta accagcggtt tatgaatgtg
    16921 gaaggcctgg ttaaaattga agaagttaca ggtaaaaaga aaagagaagt gaaaatacac
    16981 cgggtttttt atgcttcggc acttgaccaa agaaaagata cccttgaagt acgaacgata
    17041 acagaagact tttctacccg caaatatttt ggcgaccccg accatttcag gctgcttgtt
    17101 acagagatgc tgaaactgca gcagaatatc tatgacgccc aatactccat gcagtaccgg
    17161 aagataccca aaccggcaaa actcttctga ggaaaatagt gaaaaaataa tgggtattca
    17221 ccccttcaaa acccttccat tcccttagct ttgcgcaatt tgttttttta taacgattac
    17281 tgcctgcaca gccaaaaaac aaaaaatgcc aaaagacaat tccatccaat cagtactgat
    17341 tatcggctcc ggccccataa ttatcggaca agcctgtgaa tttgattact ccggtaccca
    17401 ggcggcaaga agcctgcggg aagaaggcgt aaaagtgatc ctgatcaaca gtaatcccgc
    17461 taccattatg acggacccga tgatggccga ccgggtttac ctgctgccgc tgacagtaga
    17521 aagtattgaa cagatactcc aggaaaacca gattgatgct gtattaccaa cgatgggtgg
    17581 ccaaaccgcc ctgaacctgg ccaaggaagc agaagaactg ggcttgtgga aacaatataa
    17641 tgtgcggttg attggtgtgg atattaaagc cattgaaaaa gctgaagaca gagaaaagtt
    17701 tcgtcaatgg atgatacaac ttggcgtaaa tgtagcacag gcaaaaactg cgaattcctt
    17761 tttagaagga aaagaatttg cgcaggaaat tggcttcccg ctggtaattc gcccctcatt
    17821 tacgctgggt ggaactggtg gcggtttcgt tcatggcaaa gatgaccttg atgaagccct
    17881 gaaccgtggc ttacaagcct cgcctatcca cgaagtgctg gtggaaaagg cagtgctggg
    17941 atggaaagaa tttgaactgg aactgttgcg ggatgctgca gataatgtct gtatcatatg
    18001 cacagtagaa aattttgacc cgatgggtgt gcataccggc gactccatca ccgtagcgcc
    18061 ggccatgact ctgagtgata ccgctatgca actgatgcgt aacacggcca tccgaatgat
    18121 gcgtgacctg ggcaattttg caggcggttg taatgtacag tttgccctaa acccggatac
    18181 agaagaaatt attgccatcg aaatcaatcc gagggttagc cgttcttcgg cacttgcctc
    18241 taaagctact ggttatccaa ttgctaaaat tgccgccaaa ctagccatcg gctataacct
    18301 tgatgaactg gaaaaccaga tcaccaaaac aacatccgcc tattttgagc cggcgctgga
    18361 ttatgtgatc gtcaagattc cccgctggaa ttttgacaaa ttcaaaggtg ctgatgatac
    18421 cctgggcctc cagatgaaaa gtgtgggtga agtaatgggt atcggcagaa gttttacaga
    18481 agccgtacaa aaagcctgtc agagtttaga gaataatgca gtgggacttg gctattatgg
    18541 caaaagcctg atgcatgcag atgaattgct ggaatatatt aagacaccaa aatgggaccg
    18601 catcttccgt atcaaagatg cgctgatggc agggatctca gttagcagta tttcaaaagc
    18661 cactaacgga atagacaggt ggttcctcta tgaaattcag aagctttgca atattgaaaa
    18721 agaaattgcc aaatacggac tggaagattt accaattgaa ttgatgagag aggccaaagt
    18781 gaatggcttc agtgatgaac agatcagccg cattaccggt catggcgatg aagaagatgt
    18841 atataagaaa agaaaagcag ccggtattac cagggtttat aaaatggtgg acacctgcag
    18901 cgccgagttt gaagcgaaaa caccctattt ctattctaca tttgaaagcg gcaaccacaa
    18961 tgaaagcaaa gtttcagaca gaaaaaaaat cattgttctc ggctcaggcc ctaaccgtat
    19021 cgggcaggga attgaattcg actattgctg tgtacatggc cttctcgcta ttaaagaatg
    19081 tggctacgaa gccatcatgg taaactgtaa ccctgaaacg gtaagtacgg attttgacat
    19141 ggcggataaa ctgtattttg agccggttta ctgggaacat ctgtgggaaa tcattgagca
    19201 tgaaaaacca tttggtgtta ttgtacagtt gggaggacaa actgctttaa aactcgccga
    19261 gaaactacat gaaaaaggta tcaacatcat cggcacatct tttaacagcc ttgacatagc
    19321 cgaagaccgg ggccgcttca gcgatatgct gaaagaactg gatattcctt atcctaacta
    19381 tggtacggcc tacacagtag atgaagccat tgaggttgcg aataaagtgg gttacccggt
    19441 actggttcgt ccttcatacg tattaggcgg gcagcgaatg agaattgtac tgaatgatga
    19501 ggaagtagag aaagccgtgg tgagcctgct gaaacatata cctggcaata agattctgat
    19561 cgaccacttt ctcgaccgtt gccaggaagc agagatagac gccatttgcg atggagaaaa
    19621 gtttcatgta atgggaatta tggagcatat tgaaccggcg ggtatccata gcggagatag
    19681 caatgccgtg cttccttact tcaatctttc accgctggaa gtgcaggata tggtggatta
    19741 tgccaagaaa attgcacttg cactgaatgt aaaaggcctg atcaatattc aatttgcaat
    19801 caaagatggt aaagtgtatg tgattgaagc taaccccagg gcatcaagga ctactccctt
    19861 tattgc
//
LOCUS       DAORLS010000018        18995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1553_length_18995_cov_1.933052, whole genome shotgun sequence.
ACCESSION   DAORLS010000018 DAORLS010000000
VERSION     DAORLS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18995
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1553_length_18995_cov_1.933052"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PK977_02865"
     CDS             complement(<1..247)
                     /locus_tag="PK977_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17075.1"
                     /translation="MAQLHNRISRKELKEAVLNDPTPRTTISFYCYFKIQNPQEFRNV
                     LYRDLKEIGVLGRIYVAEEGINAQISVPSDYLEQFRSY"
     gene            328..1026
                     /gene="aat"
                     /locus_tag="PK977_02870"
     CDS             328..1026
                     /gene="aat"
                     /locus_tag="PK977_02870"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRF17076.1"
                     /translation="MQRYVSSQKKLSAIFVSVIFALEDKKLIFPPVHLAEPDGLLAMG
                     GDLSVERLVLAYKNGIFPWYEGDVILWWCPHPRFVLFPNELKINKTSKAVLKNTNFEF
                     TINRSFKEVIHQCKNIKRPGQTGTWITDEVENAYCKMHEMGYAHSAEAWNGNKLVGGL
                     YGIRLGNVFFGESMFSKQSNASRFAFLKYLQLLKEEGVQLIDCQVYTEYLESFGARMI
                     GRKEFICQIRKLIP"
     gene            complement(1030..1806)
                     /locus_tag="PK977_02875"
     CDS             complement(1030..1806)
                     /locus_tag="PK977_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17077.1"
                     /translation="MEKMLFFFLLMPLIGISQSDKHITAFEKNIPGYLGKRIYLPEPD
                     YTIDLIGEPGALKRILLIEKDSVYRKIFTRQVYAKDTLPAIDFNTSELILYSACGFCL
                     AVCDLNSGHQSCHRPACNYQYAWFVRQKQNNKRVASDQEKVMLDVLYNKQPGSSSRQT
                     SCPLLRHSDLEKQFEIENKSCYLYKINNDSIYNAVFGWYRKYMPASVPVINFEKQELL
                     VQVFCHQCLIRYVVDKKLWVKNPPHPGQCIYSARWFIADK"
     gene            complement(1856..>2395)
                     /locus_tag="PK977_02880"
     CDS             complement(1856..>2395)
                     /locus_tag="PK977_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF17078.1"
                     /translation="IDSINNESWLSNTVDPWYKSVRHQQWNDYVFNEVVPFIRNSTSS
                     ETPIITCGASFGALHSMNLFLKRPDIINGVIAMSGVYDLTEYTKGHYDDHVYFNSPMH
                     YMPNMTDHNILEQIRRSKNIHIVTGSGSYEDPTSSGRFAKILYDKSIWYELDVWGKEW
                     PHDWDTWRAMLPHYIGSRF"
     assembly_gap    2396..2419
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2862..3293
                     /locus_tag="PK977_02885"
     CDS             2862..3293
                     /locus_tag="PK977_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17079.1"
                     /translation="MESSNPEIRIILADEHTAIRKSWKKILESNPRFKILADCNDGLV
                     AQQLFNELQPDILLLDLTHSPADGLIIAEKIVQQFPGVKVIGISVSNRLHYAVKMISF
                     GARGYLTKTSPLTEIYEGIIRVFEGDTYICEEIRSRMIQPE"
     gene            3408..6026
                     /locus_tag="PK977_02890"
     CDS             3408..6026
                     /locus_tag="PK977_02890"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRF17080.1"
                     /translation="MELSKNFDHSAAEQKWYQHWLDKKYFHSEPDDREPYTVVIPPPN
                     VTGVLHMGHCLNNTIQDILIRRARMQGKNACWVPGTDHASIATEAKVVHMLRDRGIAK
                     SSLSREEFLKYAWEWKEKYGGIILQQLKKLGCSLDWDRTTFTMDPDYYQSVINVFIDL
                     YNKGFIYRGKRMINWDPKAKTALSDEEVIRKQTQQKLYHLRYKLDVPGDEFIVIATVR
                     PETIMGDTAICVHPEDERYKNLHGKFAFVPLINRRIPIITDEYVTMEFGTGALKVTPA
                     HDMNDNQLGQKHNLEVVDILNEDGTLNEQAEIFIGEDRFEARKKIAKELEEKGHIIKI
                     EDYTSEVGYSERTDVVVEPRLSLQWWVDMKKISGPALNAVMDDEIKFYPSKFKNLYRH
                     WMENIKDWCISRQLWWGHRIPAWYDAEGRYVVAATEKEARELFKKLYGIDNATIKQDE
                     DCLDTWFSSWLWPFEVFKGYSNPGNKDVNYYYPTNTLVTAPEIIFFWVARMIMAGYEY
                     KGLKPFNEVYFTGIVRDKIGRKMSKQLGNSPDLLEMIEKDGADAVRFGIMISSPAGND
                     LMWDEAGNEQGRFFINKMWNALKLVKMWEGRQTDGQLKDAENSFAVEWFEHRLNQART
                     ELDNQFKDFRLSEALKTVYSLIWDDFCSWYLEWVKPGFEQPINKAVYKKTVEFFEQLM
                     QLLHPFMPFVTEEIYHQLKAQADDLTVKQLNPVKLADTAILADGSLLKEVISGIRDAR
                     NKNQLKPKETIKLHIQSENTAAYKKLETVLCKQINASSINYTDSSIANSITVVVQKDK
                     FFIETTTLLDTSSQKLQLQKDLDYLKGFLASVDKKLSNERFVQNAKPEVIEIERKKKA
                     DAEAKIKVIEESLQTL"
     gene            complement(6035..>7334)
                     /locus_tag="PK977_02895"
     CDS             complement(6035..>7334)
                     /locus_tag="PK977_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17081.1"
                     /translation="EGNDDVFALRLSDKKIFRISNGPLGNYYVNAGNGKITWSAFTAE
                     GYQLKQVLESEIVWNEVGNAVIESLNEKYPVSHAAVPGDILLSKVPQRFFSNEKYKKS
                     TRLLNFHSWRPYYSDPIFTFSLYGENVLNTLQTELYYLYNENERTSAAGLSMTYGAFF
                     PFISVGTEYTFNRQDVINNRVRQWDQLDTRVGLNIPLNYASGQTFKSFNAGSNYVLRN
                     EFNKGQFANLLGNTSFSYLLHTISWTQQIQRAVQHIFPRFAYTVSASHRHAITSVTGY
                     QFIANGSLYVPGLLSNQNLVFTGSFQQRDTLSQVVFSNRFGYSRGYEGRYFSRMWRLS
                     ANYHFPLWHTDWGFGNILYLQRIRANAFYDFTKVYSRDKRVTADQRSVGGEIFIDTKW
                     WNQYPLTFGFRISHMLDRDQFDGFKGTVFEFVLPVSIIPR"
     assembly_gap    7335..7387
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7388..8841)
                     /locus_tag="PK977_02900"
     CDS             complement(<7388..8841)
                     /locus_tag="PK977_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17082.1"
                     /translation="MRRLLLACIFSSITLITFSQQFGGNPPSLKWKQISTDTVRVIYP
                     AGLDSHAQRVASVVHYLAANKPFSLGDQLKKVDIVLQNQTTIANGYVQLGPFRSEFFL
                     TPEPVNFNQGSLSWTDQLALHEYRHVQQFNNFNNGLSKLMKVLFGEDGYTLAINASIP
                     DWFYEGDAVYQETAVSKQGRGRLPLFMNAYPSLWQAGKKYSWMKLRNGSLKDYVPNHY
                     NLGYLLVNYGYQKYGIDFWKKVTRDASAYKGLFYPFQSAIKRHAGIDYKTFTKQAFDF
                     YKLQTDTVVKFENFLIPVKKSYVTSYYFPYNAGSDSLIYLKSSYRQRPVFYIKDKEGE
                     HRLKTRDISIDEQFSYSNGKIVYSAYETDVRWGWRDYGVIKLLDVKTGKQQTLTKKSK
                     YFTPDISSDGSKVVAVQNHNDGSSELHVLNTSDGKVLRRIHSSEIAVFTDPKFIDQDN
                     LVTPVRLRDGKMCLATAEISTGNIIRLTPPTFNVV"
     gene            complement(8875..10302)
                     /locus_tag="PK977_02905"
     CDS             complement(8875..10302)
                     /locus_tag="PK977_02905"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRF17083.1"
                     /translation="MPHEAISVFDIFKIGVGPSSSHTLGPWRAAQRFVKAIRESNRLY
                     EVKSVRVLLYGSLAKTGKGHGTDVAVQLGLCGDDPVTFAVEQIASKISDIATMKKILL
                     LGLHEVDFNPAEDIEFLITENLPYHPNAVTFLADMVSGESIAETFYSIGGGFVVKENE
                     EVSGAQDEQLPFPINTADDLLHWCIKTGMSISEIVLENEHIWRSEDETRAGLLNIWRV
                     MKECIYRGCHSPGMLPGGLNEKRRGAELNKKLLAGKSYNDYESWLKAIRSGGNDFKYT
                     LDWVSCFALAVNEENASFGRVVTAPTNGAAGVIPAVLQYYIAFCDGNSEEKIIHFLLT
                     AAEIGSIFKKGATLSAAMGGCQAEIGVSSAMAAAGLTECMGGTQKQVMMASEIAMEHH
                     LGLTCDPIAGLVQVPCIERNTMGAIKAITASQLALQSTPDFAKVSLDAVIKTMWDTAK
                     DMDFKYKETADGGLAVNVPLSLPEC"
     gene            complement(10354..10824)
                     /locus_tag="PK977_02910"
     CDS             complement(10354..10824)
                     /locus_tag="PK977_02910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF17084.1"
                     /translation="MIKNLQLTAVFVTMMVTFSGCLKDNSCDPKSVESEEAAIQAYAA
                     ANSINAVKHSSGLYYQIINPGSGPTPTVNSVVSARYVGKLTDGTIFDSQTVNPVSFPL
                     GNTILGWQLGIPLVQKGGTIKLIIPSALAYGCTGQGPIPGNSILFFEVNVVDVQ"
     gene            complement(10827..11168)
                     /locus_tag="PK977_02915"
     CDS             complement(10827..11168)
                     /locus_tag="PK977_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17085.1"
                     /translation="MLTKEEADFIVYWEQNRMKKKKVLRQLYVGLPLAVVMVIAIFAN
                     FFSGWYKRAEMVMRREQSSLILVLMIAAILIVIFVVIFSARHRWDINEQRYRELLFRK
                     DNTNFEITEKE"
     gene            11286..12215
                     /locus_tag="PK977_02920"
     CDS             11286..12215
                     /locus_tag="PK977_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17086.1"
                     /translation="MKITTLLAQYLYTKKQLYLPGIGIFTLDNDIVPALEKDKHNKTS
                     ALEGVSFLHDRTVKEDPDLIAFISEHSGKIKALASADLYSHLELASQFLNIGKPFLFE
                     GIGSLVKTQTGEFSFTPGQVIPDRMDSAVLREYGKETISEEPVGDYKSVFYNKHTKSA
                     WKKPAAIGLILLGFGLAIWGGYTVYKKRTERSEKEETVVSSDKNETNRNQSTISKSPD
                     TTIQKKDTTVTSIQPPIMPAGTKKFILEVSPKERALYRYNRLKTFQWQVEMETRDSSL
                     FTLYILLPVSPADTSRVLDSLSRLNGKRVYIAQ"
     gene            complement(12244..12314)
                     /locus_tag="PK977_02925"
     tRNA            complement(12244..12314)
                     /locus_tag="PK977_02925"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12280..12282),aa:Cys,seq:gca)
     assembly_gap    12350..12368
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12440..13318
                     /locus_tag="PK977_02930"
     CDS             12440..13318
                     /locus_tag="PK977_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRF17087.1"
                     /translation="MSRIAESELIINARGAVYHLDLRPEEIAGTVITVGDPDRVKEVS
                     KYFDKIEVKRQHREFISHTGYVGKKRLTVLSSGIGPDNIDIVINELDALANIDFNTRE
                     IKRDLTSLNIIRIGTSGSLQEDIPVDSFVASTHGLGVDNLLNFYRHEQNEQEKEVLHS
                     FITHTQIHGQIGNPYITSCSASLIKHFVLDFHQGITVTCPGFYGPQGRILRLGIRNPE
                     LINRLTDFRFGQHRITNFEMETSAIYGLGKLLGHHCLAINAIVANRVKKEFSKDGQAA
                     VENLIKKFIGIFSENI"
     gene            13318..14100
                     /gene="dapF"
                     /locus_tag="PK977_02935"
     CDS             13318..14100
                     /gene="dapF"
                     /locus_tag="PK977_02935"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRF17088.1"
                     /translation="MQFHFYKYQGTGNDFILVDNRQNELGLTQQNIHRLCNRRFGIGA
                     DGLMLLQNKEGFDFEMKYYNADGKEGSMCGNGGRCMVKFAYHLGIHRELYRFLASDGV
                     HEAEIDIDGIVSLKMKDVTMIKKFHGDYILNTGSPHYIKMVNHVMELDVYKKGYEIRH
                     SKEFEDEGINVNFVEQLEEPDKIIVRTFERGVEDETYSCGTGVTAAALVCYHNENGFN
                     DVEVQTLGGHLSVEFDRVDEDRFENIWLCGPAEKVFQGDVEI"
     gene            14190..>14564
                     /locus_tag="PK977_02940"
     CDS             14190..>14564
                     /locus_tag="PK977_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRF17089.1"
                     /translation="MIQYFKNINQQTVAIDKPENGAWVNVLPPLKQEEFSELSSSLDI
                     PIDFLTDSLDIDERSRFEEEDNVKLIVIKTPTENNSFNDSDAYFITIPICIILTHNQI
                     VTVNSFENPAIKKFLNTFQNRHP"
     assembly_gap    14565..14588
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14623..>15130
                     /locus_tag="PK977_02945"
     CDS             14623..>15130
                     /locus_tag="PK977_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRF17090.1"
                     /translation="MEQLKEINMRRNTYEQKLYDASRNEHLLELMRIQKSLVYFVTAL
                     RSNEMLMMKLERTNFLGLTEEEKEILNDLIVDNSQALEMANIYTNILSSTLDAFASII
                     ANNQNQVLKRLAIITIVLTFPVLIASLFGMNVPSGFEHSPYAFYIVAFLSLGISVAIG
                     WFFFRKKIF"
     assembly_gap    15131..15170
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15171..15614
                     /locus_tag="PK977_02950"
     CDS             <15171..15614
                     /locus_tag="PK977_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF17091.1"
                     /translation="LINSERQVLVSDELIRGSYITKFPGGGLEFGEGTRDCLRREFLE
                     EMNLKVEIGEHIYTTDYFQISAFNPEHQIISIYYFAKALEPIKAPLRNIPFDFDEKQL
                     KVYEEKRETETFRFINWDNFSAESVTLPIDKIVATMVKEKLNHHP"
     gene            complement(15598..16398)
                     /locus_tag="PK977_02955"
     CDS             complement(15598..16398)
                     /locus_tag="PK977_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRF17092.1"
                     /translation="MIKTIIQSKPTKLFIILAGIFIANALIAEIIGVKIFSLEKTLGL
                     EPMHIRLLGNDLSVNLTAGVLLWPVVFIMTDIINEYYGMKGVRFLSYLTAALIAFAFL
                     IFYGAMKLVPADFFITSKQGSGVPDMEKAYNSVLGQGGFIIIGSLTAFILGQLIDVFV
                     FHKIKRATGEKRIWLRATGSTLISQFIDSFVVLFIAFYVGTRVNASGNDFVWPFGLFI
                     AVGVVNYIYKFIVAILLTPVIYLVHGVIEKYLGHEQAAAMKKAAMGDD"
     gene            16570..16722
                     /locus_tag="PK977_02960"
     CDS             16570..16722
                     /locus_tag="PK977_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17093.1"
                     /translation="MKKPIVMIVETRLSSLIQSFTNKDKVSKKNKPQMKASRDKNKSQ
                     RIGILV"
     gene            16759..18078
                     /locus_tag="PK977_02965"
     CDS             16759..18078
                     /locus_tag="PK977_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17094.1"
                     /translation="MNNLLLKKILPHVIAVVVFLVISALFCKPVLEGNVLNQHDNVGW
                     KGMAQNAFEYKEKNGHYPLWNPNLFSGMPNYQVAMEGKSVMPDMISILSLWTPKPINF
                     FFLACICFYILCLTLRINPIVGMLGSLAYAFSTYNPVIVAAGHDTQMLATAFMPLVLA
                     GIINIYEKKYWLGFALTTFAAYQQIGVNHLQVTYYFFLIAVILTLAYLVKWIKEKDWE
                     HIAIAGAITILSGLIGIAGNALILMTTSEYSKFTMRGGKDLDITSDTVKAIKTKGLDT
                     TYAFEYSLGKGETVSLIMPNAYGGYSGRYAGEESNVVKKLTDLGVPENNAVQVAQGMP
                     VYWGKLYTSGPAYLGVIICLLGLIGFVLLKTPMRWGLLAATVLGILMTWGKYLPGFNT
                     FLFEHLPLYNKFRSPAFAQVIPQFTMGIAAVLTLQQLLFGGQLQSRF"
     gene            18050..>18636
                     /locus_tag="PK977_02970"
     CDS             18050..>18636
                     /locus_tag="PK977_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17095.1"
                     /translation="MEASYKADFKKILYTAGGLFALLGIFYFSANYSSPYDPEIAQSI
                     SQQAQNEAIGQAVVNGLKADRKALFGGQLLRTLGLMIVALSVFYLYFKNTINAKIAAI
                     VLLIVSTIEITFASHLYFSNDKGPYENRQDQLFVPADDYTNTNFAKTAIDEQILKDTD
                     PNYRVLNMAGTSSGGTFSESRTSYYHKSVGGYHPA"
     assembly_gap    18637..18689
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18690..>18995
                     /locus_tag="PK977_02975"
     CDS             <18690..>18995
                     /locus_tag="PK977_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17096.1"
                     /translation="NQQVLNMLNTKYIVYRDPQLKQEIVIPNPDAYGNCWLVKNVRVT
                     EDRVAAFKAIGTTNLKDTAIVEKSFSNLVTQPQPDSTSTIKMTKFDNDAVEYEANCN"
BASE COUNT         5882 a         3843 c         3709 g         5348 t
ORIGIN      
        1 aataactcct gaactgctca agataatcag atggaacact gatctgagca ttaattcctt
       61 cctcagctac ataaattctt cccaacaccc caatttcctt taaatcccgg tataagacat
      121 tgcggaattc ctggggattt tgaattttaa aataacagta aaacgaaatc gtcgttcttg
      181 gtgtgggatc gtttaaaaca gcttctttca actcctttct ggagatgcgg ttgtgtaatt
      241 gtgccatata atttgaattt taattcatcc cgtaaaaccg ggataggctt tgaaccccaa
      301 ttgcaggttg ggcaaaattc taaagcggtg caaagatatg taagtagtca aaaaaagctg
      361 tccgctattt ttgtatccgt gatttttgcg ttagaagata agaaactcat ctttcccccc
      421 gtacatttag ccgaacctga cggcttgctt gctatgggag gtgatctttc tgttgaacga
      481 ttagttcttg cgtacaagaa tggtattttt ccatggtatg aaggtgatgt aatactttgg
      541 tggtgccctc acccccgttt tgttctcttc ccgaatgaac ttaaaatcaa taaaacatca
      601 aaagctgtat tgaagaatac caattttgag tttacaatca acaggtcatt caaagaagtc
      661 attcaccaat gtaaaaacat aaaaagaccc ggacaaacag gaacatggat aactgatgag
      721 gtggaaaatg cttattgtaa aatgcatgaa atgggatatg ctcacagtgc agaagcatgg
      781 aatggcaaca aattggttgg tggactttat ggtataagac taggaaatgt attttttgga
      841 gaaagtatgt ttagcaagca aagtaatgct tctcgttttg cttttctaaa gtatttgcag
      901 cttctaaaag aagaaggagt tcaactgatc gattgccagg tttatactga atatctcgaa
      961 agttttgggg cccgtatgat cggcagaaaa gagtttatat gccagataag gaaacttatt
     1021 ccttaatagt catttatcag ctatgaacca acgggcactg tatatacatt gacccggatg
     1081 tggagggttt ttcacccata atttcttgtc gactacatat ctaattaaac actgatgaca
     1141 gaatacctgt acaagcaatt cctgtttttc aaaattgatc accggaacag atgcaggcat
     1201 atactttcga taccatccaa aaacagcgtt ataaatcgaa tcattgttaa ttttatagag
     1261 gtagcatgat ttgttttcga tttcaaattg tttttctaag tcggaatgtc ttaacaaagg
     1321 acatgaagtt tgtcttgacg atgatcctgg ttgcttgtta tataaaacat ctagcataac
     1381 tttttcttga tcgcttgcca ctcttttatt attttgtttc tggcgaacga accaggcata
     1441 ttgataatta caggcaggac gatgacagga ttggtgccct gaatttagat cacaaactgc
     1501 gaggcaaaac ccacaggcag agtataaaat caactcagat gtattaaaat caatggccgg
     1561 cagtgtatcc ttagcatata cttgacgggt aaagattttg cgataaactg agtccttttc
     1621 tatcaacaat attcttttta aagcacctgg ctctccaatt aaatcgatgg tataatcggg
     1681 ttccggcaga tagattcttt tacctaaata accggggatg ttcttttcaa aagcagtaat
     1741 atgcttgtca gattgggaaa tacctatcaa aggcattaac aggaaaaaga aaagcatctt
     1801 ttccataaac catatttagc ataaagatgc ttcaggtggt ttgtttacat atcatctaga
     1861 acctcgaccc gatatagtga ggcaacatgg ctctccatgt atcccagtcg tgtggccatt
     1921 cctttcccca tacatccagt tcataccaga tactcttgtc atacaaaatt tttgcaaacc
     1981 ttccgctaga tgtagggtct tcataagaac cgctaccggt tacaatatgg atattcttac
     2041 tacgacgtat ctgctccagt atattatgat cggtcatatt gggcatataa tgcataggac
     2101 tattgaaata aacatgatca tcataatgcc ccttcgtgta ctcagtaaga tcgtaaaccc
     2161 cactcatcgc aattacgcca ttgattatgt caggcctttt cagaaacagg ttcatgctat
     2221 gtaaggcccc aaatgaagcc ccgcaggtga tgatgggtgt ttcagaacta gtgctgtttc
     2281 tgataaaggg tactacttca ttaaagacgt agtcattcca ctgttggtgg cgaacagatt
     2341 tataccaggg gtctacagta ttgcttagcc agctttcgtt gttaatactg tctatnnnnn
     2401 nnnnnnnnnn nnnnnnnnna actccagcct ctataaacgg tgcaatatga ttcatcaact
     2461 gaaacctctc atattcaagg taatcagctg cggctgttgg aaccagtaga agggcaaacc
     2521 catagtctcc ataagtagct atcggcattt ctttgttaag cgacgggctg tgccaacttg
     2581 caatttcttt cttcataatt tttttccgct ttgcagattt ttgcgaagat aactctaatg
     2641 ccgaagccta aaaaagtcaa acttatattt atatatctaa gtgtaaatca gtagttttta
     2701 aaaacaatag aaaaaatact tggtaattta tcatagtgta tttactttta ttttacagta
     2761 tttttactat ccaataaatc ttaagagcca ccacccaaat ccactctcct taataattcc
     2821 ggcaaaactt taaagtatta cccattttga aatgttaagg aatggaaagt agtaaccctg
     2881 agatccgtat catactggct gacgaacata cagctatcag aaagtcctgg aaaaaaatat
     2941 tagagagtaa tccccggttc aaaattcttg ctgattgtaa cgatgggctg gttgcccaac
     3001 aattgttcaa tgaacttcag cccgatatac tattactgga tcttacacat tcgccggctg
     3061 atggcctgat aatagcagaa aaaattgttc agcagtttcc tggtgtaaaa gtcataggta
     3121 tttctgtcag taacagactt cattatgctg taaaaatgat aagttttggt gcaagaggtt
     3181 acttaactaa aacctccccg cttacagaga tatatgaagg aataatccgg gtgtttgaag
     3241 gagatactta tatctgtgaa gaaatcaggt cccggatgat tcagccggaa taaagaactg
     3301 tacaatcctt cgtttcgcca tcttcttaaa acccggtatt tttgcggcct tacacgttag
     3361 tccattcgac tggctttcat tacattattt aatctcaaga gagaagcatg gaattgagca
     3421 agaattttga ccacagtgcc gctgaacaga aatggtacca gcattggctg gacaaaaaat
     3481 attttcatag tgaacctgac gatcgtgaac cttatacagt tgttatccca ccacctaatg
     3541 taaccggtgt cctgcacatg ggacactgcc tgaataatac tattcaggac atattgattc
     3601 gtcgtgcccg catgcaggga aaaaatgctt gctgggtacc cggtacagac catgcctcta
     3661 ttgcgaccga agccaaagtg gtacatatgc tccgggacag aggtattgcc aaatcatcct
     3721 taagcagaga ggagttttta aaatatgcct gggagtggaa agaaaaatac gggggcatca
     3781 tcctgcagca attaaaaaaa cttggatgca gtcttgattg ggaccgtacc acttttacca
     3841 tggatcctga ttattaccag tctgtaatta atgtttttat tgatttatac aacaaaggtt
     3901 tcatttaccg tggcaaaagg atgatcaact gggacccaaa ggcaaaaaca gccttgagtg
     3961 atgaagaggt gatccgtaaa caaacacagc aaaagctata tcatttaaga tacaaattag
     4021 atgtgccggg tgacgaattc atcgtaattg ctacagtgag acctgaaacc atcatgggtg
     4081 atactgccat ttgtgtgcat cctgaagatg aaagatacaa aaacctgcat ggcaaatttg
     4141 cctttgttcc actaatcaac cgacgtattc ccattattac tgatgaatat gtaacaatgg
     4201 aatttggaac aggagctttg aaagtgacac ctgctcatga catgaatgac aaccagcttg
     4261 gacaaaagca taaccttgaa gttgttgaca ttctaaacga agacggaacg cttaatgaac
     4321 aagctgaaat attcataggt gaagaccgtt ttgaagcccg caagaagatt gcaaaagaac
     4381 tggaagaaaa agggcacatt ataaagattg aggactatac cagcgaggtg ggttatagcg
     4441 aaagaacaga tgtagtggtg gaaccccgtc tttctttgca atggtgggtt gacatgaaga
     4501 aaatttccgg accggcttta aatgccgtaa tggatgacga aattaagttt tatccttcca
     4561 agttcaagaa cctgtaccga cactggatgg agaatatcaa agactggtgt atctcccggc
     4621 aactctggtg gggacatcgt ataccggcct ggtacgatgc cgaaggcaga tatgtagttg
     4681 ctgccacgga aaaagaagca agggaacttt tcaaaaaact atatggaata gacaatgcca
     4741 caattaagca ggatgaggac tgccttgata cctggttctc cagctggctt tggccctttg
     4801 aggtattcaa aggatatagt aatccgggca acaaagacgt taactattat taccctacta
     4861 atacactggt aacagcccct gagatcatct ttttctgggt agccaggatg atcatggctg
     4921 gttatgaata caagggcctg aaacctttta acgaagtcta ttttaccggt attgtaagag
     4981 acaaaatcgg cagaaaaatg agtaagcagt tgggtaattc acccgatttg cttgaaatga
     5041 ttgagaaaga tggcgccgat gccgttcgtt ttggcataat gatatcatct cctgccggta
     5101 atgacctgat gtgggatgaa gcaggcaatg aacagggtag atttttcatc aacaaaatgt
     5161 ggaatgcgct gaagctggtg aaaatgtggg aagggcggca gacagatggg caattaaaag
     5221 atgctgaaaa cagctttgca gtagaatggt ttgaacaccg ccttaaccag gcaagaacag
     5281 aattggataa ccagttcaaa gacttcaggc tcagcgaagc attgaaaacg gtttactcac
     5341 ttatttggga tgatttctgc agttggtacc tggaatgggt aaaaccggga tttgaacaac
     5401 caatcaataa agctgtttat aaaaaaacag tggaattctt tgagcagctt atgcaattac
     5461 tgcatccttt catgccattt gtaacagaag aaatatacca tcagctaaaa gctcaagccg
     5521 atgatcttac agtaaagcag ttaaacccgg taaaattagc tgacaccgct attcttgcag
     5581 atggttcctt actgaaagaa gtaatctccg gcataaggga tgccagaaat aagaaccaat
     5641 tgaagccaaa agaaacgatt aaattacaca tacagtctga gaatacagct gcgtataaaa
     5701 agcttgaaac tgttttgtgt aaacagatta atgcatcctc aattaattat actgacagta
     5761 gtatagctaa tagcattaca gtagtagtcc aaaaagataa atttttcatt gaaacaacaa
     5821 cactacttga taccagttcg cagaagctac aattgcaaaa agatcttgat tatctgaaag
     5881 gattcctggc atcagtagat aagaaattaa gcaatgaaag atttgtacaa aatgccaaac
     5941 ccgaagtcat tgagatcgaa cgaaagaaaa aggcagatgc ggaagccaag attaaagtga
     6001 ttgaagaaag tcttcaaact ctttaaagca tagcttatct cgggataata cttacgggca
     6061 gtacaaattc aaatacggtt cctttgaaac cgtcaaactg gtcacggtca agcatatggc
     6121 taatccggaa accaaaggtt aacgggtatt ggttccacca tttggtatct atgaatattt
     6181 caccccctac acttctctgg tcggcagtca cccttttatc tcttgaatag actttagtaa
     6241 aatcataaaa tgcattggcc ctgatacgct ggagatacaa aatatttcca aatccccagt
     6301 ctgtatgcca caaagggaaa tgataattgg ccgacaaccg ccacatacgg gaaaagtaac
     6361 gtccttcata gcctcttgaa taaccaaagc gattggagaa tactacttgc gataaagtat
     6421 cccgttgctg gaaagaacct gtgaatacaa gattttgatt actaagtaat cccggaacat
     6481 ataacgaccc gttagcaatg aattgataac cggttacact ggtaattgca tggcgatgac
     6541 tggcagaaac cgtataagca aatcggggga aaatatgctg taccgctctc tgtatctgtt
     6601 gcgtccatga aatggtgtgc aggagatagc taaaagaagt attacccaat aagtttgcaa
     6661 actggccttt gttaaactca tttcttaaca cataattact gcctgcatta aagctcttaa
     6721 aagtctggcc gcttgcataa ttcaacggta tatttaaccc tactcttgta tccagctgat
     6781 cccattggcg aacacggtta ttaattacat cctgccggtt aaatgtgtat tctgttccca
     6841 cactgataaa agggaaaaaa gctccatatg tcatgctcaa gcctgcggca ctggttcttt
     6901 cgttttcatt atagagataa tacaattcag tttgtaacgt attcagtaca ttctcaccat
     6961 acaacgagaa agtaaagatc ggatcggagt aataaggtct ccagctatga aaattcagta
     7021 accgtgttga ttttttgtac ttttcattgg aaaaaaaccg ctgaggcact ttggatagca
     7081 aaatatcacc aggcacagca gcatgactta ccgggtattt ttcattcaat gattcaatta
     7141 ctgcattacc aacttcattc catacaattt cactttccag tacttgtttc aattgatatc
     7201 cttcagcagt aaaagctgac caggtaattt ttccatttcc tgcattcaca taataattgc
     7261 caagggggcc gttgctgatt ctaaatattt tcttatcgct tagccgcaga gcaaaaacat
     7321 catcattccc ttcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnacc acattaaaag ttggcggagt aaggcggata atgttcccgg tggatatctc
     7441 agcagttgcc aggcacattt taccgtctct tagtcttact ggtgtgacaa gattatcctg
     7501 atcaataaat ttgggatccg taaatactgc tatctcagat gaatgaatac gcctcaaaac
     7561 ctttccatca ctggtattaa gcacatgcaa ttcactgcta ccatcattgt gattttgaac
     7621 agccaccact tttgaaccat cggaagaaat atcgggggta aagtattttg atttttttgt
     7681 cagcgtttgc tgtttccccg tttttacatc taataattta ataacaccgt aatctctcca
     7741 gccccagcgt acatctgttt cataagccga ataaacaatc tttccgttac tgtaggagaa
     7801 ttgttcatca attgaaatat cccttgtttt cagacgatgt tcaccctcct tatcttttat
     7861 gtaaaataca ggtcgctgcc ggtaactgga tttcagataa ataagcgaat ccgacccggc
     7921 attgtaaggg aaataataac tggtaacata actcttctta acaggtatta aaaaattttc
     7981 aaactttact actgtgtctg tttgtaattt ataaaaatca aaagcctgct tagtgaaggt
     8041 tttgtaatca attcccgcat gtctttttat tgcagactgg aaaggataga atagaccttt
     8101 atatgcactg gcatcccggg ttactttttt ccaaaaatca attccgtatt tctggtaccc
     8161 ataattgacc agtaaatagc caaggttgta atggttgggt acataatctt ttaaagaacc
     8221 gtttcgcagt ttcatccagg agtacttttt cccggcctgc cagagagaag gataagcatt
     8281 cataaaaagt ggcagccttc cccttccctg cttacttact gcagtctcct ggtacacagc
     8341 atcgccttca taaaaccagt cgggtatgga agcattaatg gcaagggtat aaccatcttc
     8401 tccaaaaagc actttcatca acttactcaa tccgttatta aaattgttga attgctgcac
     8461 atgcctgtat tcatgcaatg ccaattgatc tgtccagctt aaacttccct ggttaaaatt
     8521 aacaggttca ggagttaaaa agaactcact tctaaaaggt ccaagctgaa catagccatt
     8581 agcgatggtt gtctggtttt gcaatacgat atctactttc ttcaattgat cacccaggga
     8641 aaatggcttg ttggcagcaa ggtagtgaac aactgaagcc accctttgtg catggctgtc
     8701 aagtccggca ggataaatta cccttactgt atctgtacta atttgtttcc acttaagtga
     8761 gggagggttt cctccgaact gttgagaaaa agtgattaaa gtaattgatg aaaagataca
     8821 ggcaagtaaa agtctccgca tggtaaggga tatttaatgg aagttactag taaatcagca
     8881 ttcgggtaag ctgagcggta cattaacagc cagacctccg tcagccgttt ctttatattt
     8941 aaaatccatg tcttttgcag tatcccacat ggttttaatc actgcatcca atgaaacttt
     9001 ggcaaaatca ggagtgcttt gcaaagccaa ctgcgatgca gtaatggcct tgatagctcc
     9061 catcgtattt cgctcaatac aaggtacctg gaccagccct gcaatcggat cacaagtaag
     9121 gcccaaatga tgttccatcg caatttcaga ggccatcatt acttgttttt gtgtacctcc
     9181 catacattct gtgagacctg cagctgccat agctgaagaa acgccgatct ccgcctggca
     9241 cccacccatt gctgctgaaa gtgtagcccc ctttttaaaa atgctgccga tctctgccgc
     9301 agtcaaaaga aaatgaatta ttttttcttc actattccca tcgcaaaaag caatataata
     9361 ctgaagtaca gctggaatca ctcctgctgc cccattggta ggcgctgtga ctactctgcc
     9421 aaacgaagca ttctcttcat taactgccag ggcaaaacaa ctaacccagt ccagtgtgta
     9481 tttaaaatca tttcctccgg aacggattgc tttcaaccat gattcgtaat cattgtatga
     9541 ttttccggct aatagtttct tgtttaattc agcccctctc cttttttcat tcaatcctcc
     9601 gggaagcata ccaggtgagt gacaaccgcg gtatatacat tctttcatga cccgccatat
     9661 attcaacaaa cctgccctgg tttcatcttc acttctccag atatgttcgt tttccagtac
     9721 aatctcactt atcgacatgc ctgtttttat acaccagtgc aacaggtcat cggctgtatt
     9781 aataggaaaa ggaagttgtt cgtcctgtgc gcctgaaacc tcctcatttt ccttaaccac
     9841 aaacccgccg ccaatagaat aaaaagtttc tgcaattgat tcaccagaaa ccatatcggc
     9901 aagaaaagta actgcattgg gatgataagg caggttttct gtaatcagaa actcaatatc
     9961 ttctgcagga ttaaaatcaa cctcatgcag acccagcagg aggattttct tcatagttgc
    10021 aatgtcactg atctttgatg cgatttgttc tacagcaaat gttaccgggt catcaccgca
    10081 taaacccaac tgtacagcta catcagttcc atgtcccttg ccggttttgg ccagggagcc
    10141 ataaagtaac accctgacag atttcacttc ataaagcctg ttactttccc tgatagcttt
    10201 cacaaaccgc tgtgccgccc gccagggccc cagcgtatga gaacttgaag gaccaacgcc
    10261 tattttaaaa atgtcaaaaa cggagatggc ttcgtgtggc aaggtgaaaa atttaggtaa
    10321 gtataaataa caatcggtta cttaatctgc tgcttattga acgtctacta cgtttacttc
    10381 aaaaaataaa attgaattac ccggaattgg gccctgccca gtgcaaccat atgctaaagc
    10441 tgatggaatt atcagcttaa tagtgccgcc cttctgaacc agcggaatac ccaactgcca
    10501 gcccaaaatg gtattaccca acggaaaaga aacagggttc accgtttgtg aatcgaaaat
    10561 agttccgtct gtcagcttgc caacataccg ggcagatact actgaattga cagttggcgt
    10621 aggtccgcta ccgggattta taatctgata atacaggccg ctcgaatgct tcaccgcatt
    10681 aatcgaatta gcggcagcat aggcttgtat cgccgcttcc tcactttcta cacttttggg
    10741 gtcacaacta ttgtctttca aacaaccaga gaatgtgacc atcatcgtta caaaaacggc
    10801 agttaactgt agattcttaa tcatatttat tctttttcag ttatttcaaa attagtgttg
    10861 tctttcctga acagtagttc cctgtaacgt tgttcgttga tatcccaccg gtggcgggcg
    10921 gaaaaaataa caacgaatat aacgatcaga atggctgcaa tcatcaaaac aagaatcagc
    10981 gatgactgct ctcttctcat caccatttct gcccttttat accaccctga gaaaaaattt
    11041 gcaaaaattg ctatcaccat tactacagcc agcggcaaac caacatacaa ttgccgtagt
    11101 acctttttct ttttcatccg gttttgttcc cagtaaacga tgaagtcagc ttcctctttt
    11161 gttaacatac atttattctc ctattctttc acaatcttag aatgggcaaa ggtaaatcac
    11221 aaaggacttt cacccttgca ttgccggaac aaaatatata tcttgtgctt cttaactcca
    11281 tgactttgaa aatcaccacc ctgctggcgc aatatctcta taccaaaaag cagctttacc
    11341 tgcccggtat tggtatattt acccttgata atgatatagt gccggctctg gaaaaagata
    11401 aacacaacaa aacatctgca ctagaaggcg tttcatttct gcatgaccgg actgttaaag
    11461 aagatcctga tcttattgcg tttatttcgg aacatagtgg taaaataaaa gctcttgctt
    11521 cagcagacct ttattctcat cttgaactgg ccagccagtt tctgaatatt ggtaaacctt
    11581 ttcttttcga aggaattggc agccttgtga aaacccaaac cggcgaattc agttttaccc
    11641 ccggacaggt aatcccggat cgaatggatt cagccgttct aagggaatac ggaaaggaaa
    11701 caataagcga agagccggtt ggcgattata aaagtgtatt ttataataaa cacacaaaat
    11761 ctgcctggaa gaagcctgcg gccataggtc tgattctgct gggctttggt cttgctattt
    11821 ggggaggata tacagtttat aagaaaagaa ctgaaagaag tgaaaaggaa gaaacagtag
    11881 tgagctctga taaaaatgag acaaaccgga atcaaagtac aataagcaaa tctcccgaca
    11941 ctactattca aaaaaaagat acaactgtta catcaattca acctccaata atgccggcag
    12001 gtactaaaaa attcattcta gaagtttcac cgaaggaaag agctttgtac aggtataacc
    12061 ggttaaaaac atttcagtgg caggttgaga tggaaacaag ggactccagt ttgtttacac
    12121 tttacattct tttaccagta tcgcctgccg ataccagccg tgttttagat tctttaagcc
    12181 ggctgaacgg aaaacgggtt tacattgcac aataaaaaaa gccatcttac gatgactttt
    12241 tagaggtccc gaccagattc gaactggtgt aggagctttt gcagagctct gcctagccac
    12301 tcggccacag gaccatttca attatttcag attgtagaat taagactttn nnnnnnnnnn
    12361 nnnnnnnntt agatttgaaa tccgaaacgg ttgcaaatat acattttttg actaattcgc
    12421 taacaaataa taaaagaaca tgagcagaat tgcggaatcg gaactgatca ttaatgcaag
    12481 gggtgctgtg tatcatttgg acttaaggcc agaagaaatt gccggtactg tcattaccgt
    12541 aggtgaccct gaccgggtaa aagaagtaag caaatatttt gacaaaatcg aagttaaaag
    12601 acaacaccgg gaatttattt ctcatacagg ttatgttggg aaaaaaaggt taaccgtttt
    12661 gtcctccgga ataggcccag ataatattga tattgtgatc aacgaactgg atgcactggc
    12721 caatattgat tttaacaccc gggaaatcaa aagagatctc acttctttga atataatccg
    12781 tattggcact tcgggctctt tacaggaaga tataccagtt gacagttttg tagcctccac
    12841 tcatgggctt ggcgtagaca acttgctgaa tttctaccgg catgaacaga atgaacagga
    12901 aaaagaagtg ctccattctt tcatcaccca cacccaaata catggacaga tcgggaatcc
    12961 ttatatcacc agttgttctg cttcgctgat caaacatttt gtactagatt ttcaccaggg
    13021 aataacggtc acttgccccg gcttttatgg cccgcaggga aggatactgc gcctgggtat
    13081 ccgcaatcct gaactgataa atcgccttac ggattttcgt ttcggccagc accggataac
    13141 taatttcgaa atggaaactt cagccattta tggacttggt aaactgcttg ggcatcattg
    13201 cctggctatc aacgcaatcg ttgccaacag ggtgaaaaag gagttctcca aagatggaca
    13261 ggctgctgta gaaaacctta tcaaaaaatt tattggcata ttcagcgaaa acatctgatg
    13321 caatttcatt tttataaata ccagggaaca gggaatgatt tcattttggt tgataatcgc
    13381 caaaacgagt taggtttaac acaacaaaat atccataggc tttgcaaccg gcgctttggc
    13441 attggcgctg acgggctgat gctgcttcaa aataaagaag ggtttgattt tgaaatgaag
    13501 tattacaatg ccgatggtaa agagggcagt atgtgtggca atggtggccg gtgcatggta
    13561 aaattcgcct accacctggg catccaccgg gagttatacc ggttcctggc atccgatggt
    13621 gtgcatgaag cagaaattga cattgacgga atagtttcat tgaaaatgaa agatgtcacc
    13681 atgattaaga aatttcacgg tgactatatt ttaaataccg gctcccctca ttatatcaaa
    13741 atggtaaatc atgtaatgga gctcgatgtt tataaaaagg gttacgagat tcggcatagc
    13801 aaggaatttg aagatgaggg aattaatgta aatttcgtag agcaactgga agaaccggat
    13861 aaaattatag tcaggacctt tgaaagaggc gtagaagatg aaacctattc ctgcggcacc
    13921 ggagttacag ccgccgccct tgtttgttat cacaatgaaa atgggttcaa tgatgtggag
    13981 gttcagactc ttggcggaca cctgtctgta gaatttgacc gggtagatga agaccgcttt
    14041 gagaatatat ggttgtgcgg cccggcagaa aaagtatttc agggagatgt agaaatataa
    14101 tttaaacagc accacaatca cttcgctctt tccgtttttt gccccttacc tttgcctgcc
    14161 cggactcaac aatctttcgg gcaccggcta tgatccagta tttcaaaaat atcaatcagc
    14221 aaactgtagc tattgacaag ccagaaaatg gtgcctgggt gaatgtattg ccaccactta
    14281 aacaagagga gttttctgaa ctgtcttcat ccttggatat tcctattgac tttctgaccg
    14341 actcacttga cattgatgaa agaagccgat tcgaagaaga agataatgtc aaactgatag
    14401 ttattaaaac gcctacagag aacaactcat ttaatgacag cgatgcttat ttcataacaa
    14461 tacctatctg tattattctt acccataacc agatcgttac cgtcaattcg tttgaaaacc
    14521 ctgccatcaa aaaattcctg aatacttttc aaaacaggca tcctnnnnnn nnnnnnnnnn
    14581 nnnnnnnngc ttaaagtttt tgaaaagatt gttcagaatt tcatggagca acttaaggag
    14641 atcaatatgc ggcgtaatac ttatgaacaa aaattatatg atgccagccg aaatgaacat
    14701 ctgcttgaac tgatgaggat acagaaaagc cttgtatatt ttgttactgc actccgatcc
    14761 aatgaaatgc tgatgatgaa actggaaaga accaatttcc tgggtttgac agaagaagaa
    14821 aaagaaatct taaatgacct tattgtcgat aattcacagg cccttgagat ggccaatatt
    14881 tataccaata tcctaagtag tacacttgac gcttttgcca gtatcattgc caataaccag
    14941 aaccaggtat taaagagatt ggccattatt acaatagtgc tcaccttccc tgtactgatt
    15001 gccagtttgt tcggcatgaa tgttccaagt ggttttgaac attcccctta tgcattttat
    15061 atcgttgcct tcctttcatt gggaatttcg gtagctattg gatggttctt tttcagaaag
    15121 aaaatattct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttaataaata
    15181 gtgagcgaca ggtattggta agtgatgaac tgatccgggg aagttacatt accaaatttc
    15241 ctggcggcgg gctggagttt ggtgaaggca cccgtgactg tctgagaaga gagtttcttg
    15301 aggaaatgaa cctgaaagtt gaaattggtg agcacattta cactactgac tatttccaga
    15361 tctccgcttt taatcctgag caccagataa tatccattta ctattttgcc aaagcacttg
    15421 agccaatcaa agctcctcta cgcaacatac cgtttgactt tgatgagaag cagctgaaag
    15481 tttatgaaga aaaaagagaa acagaaacct ttcgctttat taattgggac aatttctcag
    15541 cagaatctgt tacactccct attgataaga ttgttgccac aatggtgaaa gaaaaactta
    15601 atcatcaccc atagccgcct ttttcatggc agcagcctgc tcatgcccca ggtatttttc
    15661 aataacaccg tgcacaagat aaattacagg ggtcaaaaga atagcaacaa taaatttgta
    15721 aatgtaatta actacaccaa ctgctataaa caatccaaaa ggccagacaa agtcattacc
    15781 ggaagcattg actcttgttc ccacataaaa tgcaataaac aacacaacaa aactatcaat
    15841 aaactgggag atcagtgtag aaccagtagc tcttaaccag atacgctttt cacctgtcgc
    15901 cctttttatt ttgtgaaaga caaaaacatc aatcaactgt ccaagtataa acgccgttaa
    15961 agaaccgata ataataaaac cgccctgccc gagtacgctg ttgtatgctt tttccatatc
    16021 cggcactcca ctaccttgtt tactggtaat gaaaaaatca gcaggaacca gtttcattgc
    16081 tccataaaat atcaaaaagg caaacgcaat cagcgcagcg gtaagataag acaggaatcg
    16141 tacccctttc attccatagt actcattaat aatatcagtc ataatgaaaa ctaccggcca
    16201 tagtaagacc ccggcagtca ggtttacaga aagatcatta cccaataatc ttatatgcat
    16261 gggctcaaga cccagtgttt tctccaaaga aaaaatcttc accccgataa tttctgcaat
    16321 cagggcattg gcaatgaaaa taccagccag gattataaaa agcttggtag gtttactttg
    16381 aatgatggtt tttatcatgc tgttgcctgt gcgtacaggt agtttatata tacaatatag
    16441 ctgagcaaaa ttagcatcac aaatatgtta gccactataa gccatttagg cacatatatt
    16501 ccgggttttt tgccaaaaat ccagagcagc agataacaaa acagcgttac cggaactata
    16561 atcatcccca tgaaaaaacc gattgtgatg attgtagaga caaggctctc gtctttaatc
    16621 caatccttca ccaataagga taaagtgagc aagaaaaaca agccgcaaat gaaagccagt
    16681 cgtgacaaaa ataaaagcca gcgcatcggg attttagtct aaaatagcat tattttgtca
    16741 gcctaattgt gaaataatat gaacaatctg ctgctgaaaa agattttacc gcatgttata
    16801 gctgttgttg ttttcctggt catttccgcc ctgttttgta aacctgttct ggaaggaaat
    16861 gtattgaacc aacatgataa tgtgggctgg aaagggatgg ctcaaaacgc ttttgaatac
    16921 aaagaaaaaa atggccacta ccctctctgg aatcccaatt tgttcagtgg aatgccaaac
    16981 taccaggtgg cgatggaagg taaaagtgta atgccggata tgatcagcat attgtctcta
    17041 tggacgccta aaccaatcaa cttctttttc ctggcctgta tctgctttta tatactatgc
    17101 ctgacattac ggataaaccc gattgtgggt atgctagggt cactagccta tgccttttca
    17161 acttataatc ctgtaatcgt tgcagcgggg catgatacac aaatgctggc aacagctttt
    17221 atgcctttgg tactggccgg tattatcaat atttatgaaa aaaagtattg gcttggtttt
    17281 gcactcacca catttgctgc ctaccagcaa ataggcgtta accacttgca ggtaacttat
    17341 tatttctttt taattgcagt aatactcacc ctggcttacc tcgtaaaatg gataaaagaa
    17401 aaggactggg aacatattgc tatagccgga gccataacaa ttctatctgg attgataggt
    17461 attgcaggta atgctcttat actgatgacc acttcagaat atagcaaatt cacaatgcgg
    17521 ggaggtaaag atcttgacat tacaagcgac acagtaaaag ccatcaaaac aaaagggctt
    17581 gatacaactt atgcttttga atacagcctt ggtaaaggag aaacggtgtc attgattatg
    17641 ccgaacgcct acgggggtta tagcggtcgt tatgccggtg aagaatcgaa tgtagtaaag
    17701 aaattaactg atttgggtgt accggaaaac aatgctgtgc aggtagcaca aggcatgcct
    17761 gtttactggg gaaaattgta cacatccggc ccggcttacc tcggagttat aatttgcttg
    17821 ctcggactga ttggttttgt tttattaaaa acgccgatgc gatggggctt gctggcagct
    17881 actgttttgg gtattttaat gacatggggc aaatatcttc cgggttttaa tactttctta
    17941 ttcgaacatc ttcctttata caacaaattc cgttctcctg cttttgcaca agtgatcccc
    18001 cagttcacaa tgggaattgc cgcagtactt acactgcaac aattactttt tggaggccag
    18061 ttacaaagca gattttaaaa aaatactata tacggcagga ggtttatttg ccctgttagg
    18121 tattttttac ttctccgcaa attattcatc accatatgat ccggagatag cacaaagtat
    18181 ctcgcagcag gcacaaaacg aagcaattgg gcaggctgtc gtaaatggat tgaaagctga
    18241 cagaaaagcg ctgtttgggg gacaactact aagaactcta ggcctgatga tagttgcgtt
    18301 gagtgtgttc tatctttatt tcaaaaatac aattaatgca aaaattgcag ccattgtatt
    18361 attgattgta agcaccattg aaatcacatt tgccagccac ttatatttta gtaatgacaa
    18421 aggcccttat gaaaacaggc aggatcaatt gtttgtacct gctgatgatt acactaatac
    18481 caactttgct aaaacggcta tcgacgaaca gattttaaaa gataccgatc ctaattatcg
    18541 tgtattgaat atggcaggaa ccagtagtgg cggcacattc agtgagtcca gaacgtcata
    18601 ttatcataaa tctgtaggcg gttaccatcc ggctaannnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc caaccagcag gtattgaata tgctgaatac
    18721 aaagtacatc gtctaccggg atcctcagct aaaacaggaa attgtcattc caaatccgga
    18781 tgcatatggc aattgctggc tggtaaagaa tgttagagtt accgaagaca gagtagccgc
    18841 ctttaaagcc attggaacta ctaacttaaa agacacagca attgttgaaa aatcattcag
    18901 taacctggtt acgcaacccc aacccgattc cacatccaca atcaaaatga caaaatttga
    18961 taacgatgca gtagaatacg aggctaattg taatg
//
LOCUS       DAORLS010000019        18952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1561_length_18952_cov_2.275388, whole genome shotgun sequence.
ACCESSION   DAORLS010000019 DAORLS010000000
VERSION     DAORLS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18952
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_18952_cov_2.275388"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK977_02980"
     CDS             <1..712
                     /locus_tag="PK977_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17097.1"
                     /translation="DHYLDIYEKYFSAYRGQPVSILEIGISQGGSLQLWKKYFGENVR
                     VYAMDINPLCKKFEEEYIKIFIGSQEDEKFLQQVCHQIPEVDIILDDGGHTMRQQIVS
                     FENLFLKVKEGGLYIVEDTHTSYWHEYHGGLKNPKSFIEYSKNLVDSLYDGHIADKRK
                     LLVNEITTHINGISFYDSIVVFEKQKRNTPFHIRKGTESIEPYIQVELKKPTLWMQVK
                     QKLFGKQDTYKNNDKGKL"
     gene            857..1729
                     /locus_tag="PK977_02985"
     CDS             857..1729
                     /locus_tag="PK977_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF17098.1"
                     /translation="METASTGEPEPKQTTSFFRLLPVTFIIIILSACENRKWQKPEYP
                     VAVVDSAAIKQQEKQRQDSMQKISIAKKKIYLTFDDGPNKGTRNVLQAVREENIPASF
                     FIVGEHVFDSPKQHQTWELLKADSVIELCNHSYTHAHNRYTSFYNNPSGVVADFQRSQ
                     QALQFSNNIARMPGRNAWRIDTIDHTDIYESSAAIDSLHAAGFNIMGWDIEWMFDHKT
                     LAPDTSVALMLRRIENMLEAEKTKTPGHLVLLAHDQAFQTEEALQLLRLVLQSLKNNP
                     DYELVLASKYPGVQ"
     gene            complement(1929..>2318)
                     /locus_tag="PK977_02990"
     CDS             complement(1929..>2318)
                     /locus_tag="PK977_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRF17099.1"
                     /translation="QRKLGVGIFGGEGFIMQKLEGDGMAFLHAGGYIVEKELKAGEIL
                     KIDTGCVVGYSPTVQFDIEFVKGIKNFMFGGEGLFFAKLQGPGKVWIQSLPISRLAGR
                     IVAYGTYKRKEEGSVLGGLGNLFDGRE"
     assembly_gap    2319..2333
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2334..2738)
                     /locus_tag="PK977_02995"
     CDS             complement(<2334..2738)
                     /locus_tag="PK977_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRF17100.1"
                     /translation="MSTAPRVNHEIDYKLYGEEMQFVEIELDQNETAIAESGSMMMMD
                     DGIQMETIFGDGSAQQPTGFFGKLVSAGKRVLTGESLFMTAFTNTAGGKRKVSFAAPY
                     TGKIIPMDLQQLGGTIICQKDAFLCAAKGVSVG"
     gene            2893..5748
                     /locus_tag="PK977_03000"
     CDS             2893..5748
                     /locus_tag="PK977_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF17101.1"
                     /translation="MKQRLMMLSAFLLILSVYVNGQAKLVEKVTKQGNEIVIPYEKYV
                     LPNGLTLIIHEDHSDPVVHVDVTYHVGSAREEIGKSGFAHFFEHMMFQGSDNVADEQH
                     FKIVSDAGGTLNGTTNRDRTNYFETVPSNQLEKMLWLEADRMGFLLDAVTQKKFEIQR
                     STVKNERGQNYDNRPYGLANETASRNLYPYGHPYSWLTIGYVEDLNRVDVNDLKNFFL
                     RWYGPNNATVSVGGDVKPAEVVKLVEKYFGGIPRGPEVKPVVVPPAVLSSDRYASYVD
                     NYAKLPLLLVVYPTVPNYHKDMGALACLAQVLGQGKNSVLYQQMIKKKTALQASTFSS
                     LSELAGEFFVQLIPSPGKSLAVLDSIYRNSLDSFEARGVTDDDIAKFKGGIESQLING
                     LQSVSGKISQLASFQTFTGNPNKIADLIKMYQSVTKEDVMRVYNEYIKGKGAVFLSVL
                     PKGQEKLIAAADNYKIDSSKYDAPDYGYAGLKYAKAKDNFDRSKIPGNGAYPTVKVPK
                     FWRKDMPNGVKMIGTESSEIPTVTMTITVPGGHLLQANDTSKIGLASMFASMMNEDTR
                     NYTAEQMAVELQKLGSNVSVSSKFDGITFSIQTLKKNLDQTLALVEERMFNAKFTPEA
                     FGRIQQQRLQGFKQAKADPAAVADNVFAKLNYGPNSILGMSEDGTEYTIANLKLEDVE
                     SYYKNYMTSKGVKVVIVGDVKQAEVLPKLAFLNKLPNKKVELPAVNAKATEIDKSKVY
                     LVDIPKAAQSQFRVGYATGLKYDATGDFYKSRLMNYALGGDFNSRLNLNLREDKGWTY
                     GARSSFTADEYTGDFEFSSGIRADATDSALIEVMKELKNYRDNGITEAELKFMKNSLG
                     QRDALLYETGIQKAGFIGRILDYNLPANYVDQQNKILANLTKQQVDAISKKMLNPEKM
                     NILLVGDKAKIIEGVKKTGYEVIELDVDGKPVQKKAF"
     gene            5904..6677
                     /locus_tag="PK977_03005"
     CDS             5904..6677
                     /locus_tag="PK977_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17102.1"
                     /translation="MKLIYTLLFLTISIFSSAQFYLSSGYSLSLPKQEMKKNIRAVHS
                     LALSGLYRLPGNMDRVQLGVDFGWGMYASTRKEQTFTFANGDITRTDVFYSSMVVQGG
                     LQTRIDLLRNKIVTPYLSGKTGYTSFYSNIFVEDPHDPGGCAPLDQRNIIKDGTIYTG
                     YGGGLQVDWSIFNRNSRKSRGWIDLTVNNIQGGNLNYINTKKLVDANNPPVNSDGKPL
                     NITFINASTQQLHEHQVAEVYTSPLRMLELKISATFLLN"
     gene            complement(6764..>8985)
                     /locus_tag="PK977_03010"
     CDS             complement(6764..>8985)
                     /locus_tag="PK977_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF17103.1"
                     /translation="VFIPVLAGTATTLAPFFPLLLWKGLIGKFMIYLPVVLILTLAAS
                     LVVAFFFNPVFAVSFMKPEGKEFEKPKKAVFRNKWFLTSLIGGILLHLPGFHGVANFL
                     LLLAILMVFNAYVLNDVIHKFQNSFLPRLMSRYERLLRWILKGKRPVWAFVSLFGLFI
                     ISLLMLVVSITTGRTKSTFFPSGDPNFVYVYLKMPVGTDVRHTDSVLHILEHRVEKVL
                     EKEKPGTPGGIVESIITNVAVNANNPRDNNRSVQSHLGRIQVSFVEFEKRHGKETRPY
                     MDKIREVVQGIPGASVEVAQEDNGPPTDPPVNIEVSGDYLENVTKIATDLYRFLDTNR
                     VEGIENLQPDVDLFNPEITVKVDRQRATMEGLSTVQIGMELRTAIFGKEVSKIKDGED
                     EYKIQLRFKEDLRKNVSDLLNMRITFMDMNTMQVKSLPLSSVASVDYTNSPGGINRKN
                     VKRTIQLQSNVLDPTMVAPINAQLETKIKEFKEKNAVPADVNIKLSGQSEQEKETSAF
                     LGKALLIALGLIFFILVLQFNSMSKPFIVLTEIFFSIIGVFLGYAITGMTVATIMMGV
                     GIIGLAGIVIKNGILLIEFTDELRSRGMRTREAAIQAGKIRIIPVLLTAVATILGLFP
                     LAVGLNIDFAGLFQHLRPNLFFGGDSVVFWGPLAWTIIFGLIFSFFLTLVMVPSMYLI
                     AERLKRPMQQFYGTKYIALLGFAGPFFFIFVGIMYLVRRIQGKKVWLGKPKPAAKKA"
     assembly_gap    8986..9097
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9098..>10253)
                     /locus_tag="PK977_03015"
     CDS             complement(<9098..>10253)
                     /locus_tag="PK977_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF17104.1"
                     /translation="ILAIIISAIGFLQFITLPKEQFPDIVVPTISVTTIYAGNSAKDI
                     ESLVTQPIEKELKGISGAKVNKIQSISQADYSLIVVEFDTDVETDIAKQKVKDAIDRA
                     MSDLPNDLTSEPNVQEFAFSEIPIMFVNISGDYDGMQLKRYADKMQDRFESLGEVNRA
                     EIVGAPEREIQVNVDPYKLTAARVSLMDVENAISRENHDITGGLIETGNMKRTLKVKG
                     QFTSVNDLYNVIVRSSAQGASVYLRDVAELKDTVKEKESFARLDGKNVVTLNIVKRSG
                     ENLIDCADKVKAEVESMQKSGELPKNLRVEFTGDTSKQTKTSFTELINTIIIGFLLVL
                     IILMFFMGVTNAFFVALSVPLSVFVAFLFIPLVSSISGINITLNFIVLFAL"
     assembly_gap    10254..10374
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10378..11586)
                     /locus_tag="PK977_03020"
     CDS             complement(10378..11586)
                     /locus_tag="PK977_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF17105.1"
                     /translation="MNNIMKISAAVFFAAVLAACGGGAKEKKGNLGDLKSRLEKLKKQ
                     KSEIDADIKKMEDEIAKADPEAAQQVKRLVAIDTLRVQDFAHYIDLQGKVSNSGIGYV
                     APKGGGGVVRAIYVKVGQKVGAGQVVVKLDDALQQQALIAAQQSTGQLKARLAQAQTI
                     YERYQNLWKQNIGAEINVINAKADVDAISAQLRAAEAQVGQAREALNMTSVTAGMSGV
                     VEEINVRVGEFFSGATQQGGQIVIVNATTLKAEVPVPDNYVAKVKKGDKVLISVPETG
                     KPAFESVISVVGASIDEKTRSFMTEAKLPSDPLLKPNQTAVMKILDYEAKGTIAVPVN
                     VVQSDENGKYVYVMEKSGDKMIARKKNVNVGEVYNGVIEIKNGLSGGELLITEGYQSI
                     YDGQAVTTGK"
     gene            complement(11618..13051)
                     /locus_tag="PK977_03025"
     CDS             complement(11618..13051)
                     /locus_tag="PK977_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF17106.1"
                     /translation="MMTFRKPTFIFFILALSISGMARAQGGGNSDSVKITKHEFSVQQ
                     AVDYALKNNVNVKNALEDVLYQEQVNREVTSNAYPKINASLGTTYNPNVATQVIPNFI
                     SPSTYQVLVDEGVRDGNGNPIQMPGDFGFIAAQFGTKYSAVGAISLTQILFDGQVFVG
                     LMARDATMRFARKKVEVTEEMIKANIHKVYYQLVVSKTQVELLDANIERLEKLLKDTR
                     IIYENGFAEKLDVDKLNVQLTNLQTEKQKVLNSISNGYYGLKVLMGMPVRDELVLTDK
                     LTDEQIKEGILENSIYKYEDRKEFQYAQIGKELNEYNIRRYKLSQIPTVLLNGQYAKN
                     AQRNKWNFFGKGDWFTISNISLNINIPIFNGFYTKSKIQQTKIQLRQTENQIEALKLT
                     IDNEVETAKNNFRSAIATMDFQKKNMELAEKVYQQTKKKYEVGTGSQTEINAAQVELK
                     TAQTSYITALYDAIISKIDYMKATGKL"
     gene            complement(13054..13740)
                     /locus_tag="PK977_03030"
     CDS             complement(13054..13740)
                     /locus_tag="PK977_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF17107.1"
                     /translation="MVNKDDGNFGNEKSFHSFAPSNFVMEVRDRIIEKANELYMQYGI
                     RSVSMDDIANGLGMSKKTLYQHFADKDALVEAVVDLHIVQMKQDCGNCKSNAKDAIHE
                     IFNTMELLMEEFSNMNPMVLYDLEKFHFKAYQKFREHKDSYLQSIVRNNLEWGIKDEL
                     YRADLNIDIISKYRMESMMLPFNVAVFPPGKYNVAETTNMILELFIYGVATLKGHRMI
                     QKYKEQRQKN"
     gene            13864..14544
                     /locus_tag="PK977_03035"
     CDS             13864..14544
                     /locus_tag="PK977_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRF17108.1"
                     /translation="MNNRFSLRSYLPKEPFRFKKVVQYFLQGLLIIAPLAITIYAIYW
                     VVSTVDNWIPIFREPIKDAQGRIIGHEVKNYGLGFVVIITAVILIGYLSSFFIQSRLF
                     NLFDRWLEKTPGIKFIYTSTRDFFEAFAGNKRKFNKAVLANVFSDEVWIVGFLTDEEM
                     AKFDMGADKVAVYVPQAYNFAGQLYILPRDKVKKIEHITSGEAMKYAVTGGVVDLEAE
                     DADQEQKH"
     gene            14583..15548
                     /locus_tag="PK977_03040"
     CDS             14583..15548
                     /locus_tag="PK977_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRF17109.1"
                     /translation="MKKSLLLIITILFSIKSFCWGFYGHRKINYHAVFLLPPEMMVLF
                     KPQIDFLSEHAVDPDKRRYAVPEEGPRHYIDIDRYGHYPYPDLPRKWEDAVAKYSEDT
                     LNTYGIVPWWLQTMLYRLTSAFKEKNQVKILRYAAEIGHYIADAHVPLHANSNHNGQF
                     SGQKGIHGFWESRVPELLAEKEWDFFIGKAEYLKNPADFIWKRVLESAAAADTVLQME
                     KELTASFPQDQKFSFEDRNGITIRQYSSAFSKAYDTRLKGMIERRMRQSIHATASFWY
                     TAWVNAGQPDLKKLNNREFTNEELKEFEVLNNSWKNAPIKGREHE"
     gene            15651..>18952
                     /locus_tag="PK977_03045"
     CDS             15651..>18952
                     /locus_tag="PK977_03045"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17110.1"
                     /translation="MKKNLLLALVCMSFVISNAQISVYELMERNDISIQEAERIANRH
                     FDTAGTGRGTGYKQFQRWLYERKFHIDENGNYLSPQTEWNRYTESRTAMGSFRTEAGS
                     WTELGPWGWNRTSGWNPGTGRLSAFAIHPANENIIYVGSPGGGIWKSVNGGLNWAPLT
                     DNNANWMSVFALTIDPLNQNIVYAGMSGSVGIIKSTDAGATWAAAGAGPSGTVRKILI
                     HPTNSSIVFATATNGIWRSINAGASWTQVHSGSKEDIEFKPDDVNIMYATGNDVYRSA
                     DNGITWTPVSAAQGITNTGRTLVSVTPANPNYVYIAQASGSLFGRMYKSTDAGLTFTT
                     TVVGSPASGTNYFGYETNGTGTSGQATYDMAMDVSPTNAAEVHIAGIICWKSLDEATS
                     FTAVTAWSLPNSIGYNHADVHGLFFINSNLYSISDGGLYKSTNNGEDWTDLSTGLGIR
                     QFYRIASSQTNATVITGGAQDNGTAAKQASGTWVDWLGADGMEGLVSPTNHLNLWGTS
                     QNGSIYRSTNGGNSYSGLPRPSPGQWVTPLAIHPTNETILYGGWTGVYKSTNSGSSWT
                     NISSGVITTTLADLAVAPSDPNYIYASNGSTLYVTTNDGATWTTRSAPATINDIAVDP
                     TNPSKIWIACNSTTNRVMVSTDAGATFTNVSANLPAIVARAVVVDDNTPRGIYVGMNI
                     GVYYKTEPDANWTNYSDNLPLVAINELEIQKNAGKIRVATYGRGVWESPMAAVPGGFV
                     FGSSTPATATCPAPSVMNVTLPTSSVGSFVNPISLSATAGVPGGTTVTFSPNPVTPGS
                     STIVSLNNANTLSAGTYTITVTGTATGATTQVTNVTFTINAGSGPAITAQPADQTVCS
                     GTNTSFSVTATGATGYQWQLSIDGGATFNNISNGGVYSNATTPTLNLTGVTAGMNNYR
                     YRCIASTLCGSTTSAAATLTVNSAPAITTQPQDVLLCTGSNHTFSITATGSGLSYQWQ
                     LSTDGGVTYNNISGANTASYSLTGITPGMNGNRYRCVVTGSCSPAANSNGATLSVVTS
                     VTVTTQPANSTVCAGANTSFTVAGSGSGIIYQWQVNTGSGFNDITNGGVYSGATTATL
                     NITGATTGMSGY"
BASE COUNT         5375 a         4240 c         3809 g         5280 t
ORIGIN      
        1 ggaccactac ctggatattt atgaaaaata cttttcagca taccgggggc agcccgtcag
       61 catacttgag atcgggatat cccagggcgg gtcactgcag ctttggaaaa aatactttgg
      121 cgaaaacgtc agggtatatg ccatggatat aaacccactg tgcaaaaagt ttgaggaaga
      181 atatattaaa atcttcatcg gctcgcagga agatgaaaaa ttcctgcagc aggtttgcca
      241 tcagataccg gaggtagata ttatactcga cgatggcggc cataccatgc ggcagcagat
      301 cgtttctttt gaaaaccttt ttttaaaagt aaaagaagga gggctttata ttgtggaaga
      361 cacccacact tcctattggc atgaatatca cggcggatta aaaaacccaa agtcttttat
      421 tgaatactcc aaaaacctgg ttgactccct gtacgacggg catatcgccg ataaaagaaa
      481 acttcttgtc aacgagatca ccacgcatat caatggtatc tccttttacg acagtattgt
      541 tgtttttgaa aagcaaaaac gcaatacacc cttccatatc cggaaaggaa ctgaatcaat
      601 agaaccctat atccaggtag agctcaaaaa gccaaccctt tggatgcagg taaaacaaaa
      661 gctttttggc aaacaggaca cctataagaa caacgacaaa ggaaaattat aaacttcaaa
      721 tcccaatccc caattaacca gtcaactaat taaccaatca ccaaatcccg ttttatccat
      781 tctttaacca aagtcattat aaaacacagt agcccgtatc tatctttagc ctgtaatcaa
      841 attacagcac cacaacatgg aaacagcatc caccggcgaa cctgagccca aacaaaccac
      901 ctctttcttt cgtcttcttc ccgttacatt tatcattatt attctttccg cctgtgaaaa
      961 caggaaatgg caaaagccgg aatacccggt agcagtagtt gacagcgccg ccatcaaaca
     1021 acaagagaaa cagcggcagg attcaatgca aaagatcagc attgccaaaa agaagatcta
     1081 cctcactttt gatgacggcc ccaacaaagg aacaaggaat gtattgcagg cagtcaggga
     1141 agagaatata cccgccagct tcttcattgt aggcgaacat gtattcgaca gccccaaaca
     1201 acatcaaaca tgggagctgc tgaaagcaga ttcagtgatc gaactttgta atcacagtta
     1261 tacccatgcc cacaaccgct acacttcgtt ttataacaat ccttccggtg tggtggcaga
     1321 tttccagcga tcacagcagg ctttgcagtt cagcaataac attgcccgca tgcccggccg
     1381 caatgcctgg cgcattgata ccattgacca tacagatatt tatgaaagca gtgctgccat
     1441 cgattcatta catgctgccg gtttcaacat tatgggctgg gatatagagt ggatgtttga
     1501 tcataaaacc ctggcgccgg atacatccgt tgcattaatg ctgcgccgta tagagaatat
     1561 gctggaagct gaaaagacca aaacacccgg ccacctggta ttgcttgcac atgatcaggc
     1621 ttttcaaacc gaagaagcgc tacaattact ccgcctcgta ttacagtcct taaaaaacaa
     1681 cccggactat gaactggtgc tggccagtaa ataccccggt gtacaataag ttgatgaaat
     1741 ttatctcccg caaacattgg agacactatg ctcacaaagc ttttcacgca tagacgcaaa
     1801 atctattatg atgaagccac agaagcactg aaacacagag aagaaaaaac ttcagtgctt
     1861 ctgtgactaa tatattttgt gttctttgtg gcttagtggg aaatataact atacatgtgg
     1921 gataatccct attcccttcc gtcaaacaaa ttacccaggc cccccagcac acttccttct
     1981 tctttgcgtt tgtaagtacc ataggccaca attcttccgg ccagccggct gatcggcaat
     2041 gattgtatcc acactttgcc cggtccctgc agtttggcaa agaataaacc ttcgccgcca
     2101 aacataaaat tcttgatgcc ctttacaaat tcaatatcaa actgtactgt tggcgaatag
     2161 cccaccacgc agccggtatc tatctttaat atttctccgg ctttcagttc cttttctact
     2221 atataaccac cggcatgcag gaaagccatg ccatcacctt ccagtttctg catgatgaag
     2281 ccctcaccgc cgaaaatgcc cacacccagc ttgcgctgnn nnnnnnnnnn nnnaccaaca
     2341 cttacacctt ttgcagcaca gagaaaagca tctttctggc aaattatagt accaccaagt
     2401 tgttgcaggt ccatcgggat gatcttgccg gtgtaaggtg cagcaaagct tactttccgt
     2461 ttacctcctg ccgtgttggt aaaagcggtc ataaaaaggc tttcacccgt cagcacccgt
     2521 ttaccggcgc tgaccagttt tccaaagaag ccggtaggct gctgggctga cccgtctcca
     2581 aaaatggttt ccatctggat accgtcatcc atcatcatca tagaaccgct ttcggcaata
     2641 gccgtttcat tctgatccaa ttcaatctca acgaactgca tttcttcacc ataaagctta
     2701 tagtcaattt catgattgac acggggagct gtactcatat aattattttt atcaaaggta
     2761 tgaagggagg aaatgaattg acaaacgcaa gtttccggct gaccagtcat tctttgtagc
     2821 taaaaatgat aaatggcaac catttgaagg ccggatttgt ttaatttgcc agaccgataa
     2881 aacaatttat ttatgaaaca acgtctgatg atgctttcag cttttctgct gattctttcc
     2941 gtttatgtaa acgggcaggc aaagctggta gagaaagtaa ccaagcaggg aaacgagatc
     3001 gttatcccct acgaaaaata tgtattgccc aacgggctta ctttaatcat tcatgaagat
     3061 cacagtgacc cggtggtaca tgtggatgtc acctatcatg tgggatctgc ccgggaagaa
     3121 ataggaaaat ccggtttcgc ccacttcttt gaacacatga tgttccaggg aagtgataac
     3181 gtagccgatg agcagcattt caaaattgta tctgatgccg gcggtacatt gaatggtact
     3241 accaaccgtg accgaaccaa ctattttgaa acagtgccca gcaaccagct ggaaaagatg
     3301 ctgtggctgg aagcagaccg catgggtttt ttgctggatg cagtaaccca aaagaaattc
     3361 gagatacagc gttctaccgt gaagaatgaa agaggacaga attatgataa ccgtccttac
     3421 ggtttggcca acgaaacggc ttccagaaac ttatacccct atggccaccc ttattcatgg
     3481 cttactattg gatatgtgga agatctgaac cgtgtagatg taaatgattt aaagaacttc
     3541 ttccttcgct ggtacggacc taataacgca actgtttcag ttggtggtga tgtaaaacct
     3601 gccgaagtgg taaagctggt tgaaaaatat ttcggtggca taccccgcgg cccggaagta
     3661 aaacctgttg ttgttccacc cgctgtatta agcagcgaca ggtatgcttc ttatgtagat
     3721 aattacgcca agcttccttt gttgctggtc gtttatccca ctgtacccaa ctatcataag
     3781 gatatgggcg ccctggcctg tttggctcag gtattggggc agggtaaaaa ctctgttttg
     3841 taccagcaaa tgataaagaa aaaaaccgct ctccaggcaa gtactttcag cagcctgtct
     3901 gaactggccg gtgaattttt tgttcagctg attccttcac cgggcaaatc attggcagta
     3961 ttggattcaa tttatcgcaa ctctcttgac tctttcgaag caagaggtgt tacggacgat
     4021 gatattgcca agttcaaagg cggtattgaa tcacagctga tcaacggttt gcaaagtgtt
     4081 tccggaaaaa tctcccagct ggcttctttc cagactttca ccggcaatcc taacaagatc
     4141 gccgacctga ttaaaatgta ccagtcggtg accaaagaag atgtgatgag agtgtacaat
     4201 gaatatatca aaggaaaagg agctgtcttt ctgagtgtat tacccaaagg ccaggagaag
     4261 ttgattgctg ccgctgataa ttacaagata gactcatcca aatatgatgc acctgattat
     4321 ggctatgcag gattgaaata tgcaaaagca aaagacaatt ttgaccgaag caagatacca
     4381 ggaaatggcg cttacccaac tgtaaaggtt cccaaattct ggagaaagga tatgccaaac
     4441 ggcgtgaaaa tgattggtac agaatccagc gagattccga cagtgaccat gactattacg
     4501 gttcccggtg gccacttatt acaggcgaat gatacttcaa agattggact tgccagcatg
     4561 tttgccagta tgatgaatga ggataccagg aattatactg cagaacaaat ggcagtggaa
     4621 ttgcaaaagc tgggaagcaa tgtctctgtt tccagcaaat tcgatggtat caccttcagc
     4681 atccagaccc tgaagaaaaa tcttgaccag acactggcac tggtggaaga aaggatgttc
     4741 aatgccaaat tcacaccgga agctttcggc cgcattcagc agcaacgatt acagggattc
     4801 aagcaggcaa aggccgatcc ggctgctgtt gctgataatg tttttgccaa gctgaattat
     4861 ggccctaaca gcatcttggg catgagcgaa gacggaacgg aatatactat tgccaacctg
     4921 aaactggaag atgttgaaag ttattacaag aactacatga catcaaaggg tgtgaaagtg
     4981 gtaatagtgg gtgatgtaaa acaagctgaa gttcttccca aactggcttt cctgaacaaa
     5041 ctgcctaata agaaagtcga gttgcctgcc gtaaacgcaa aagcaaccga aattgataaa
     5101 tctaaagtgt acctggtgga cattcctaaa gcagcccaat ctcaattccg ggttggttat
     5161 gctaccggtt tgaaatacga tgccacaggt gatttctaca aatcaagact gatgaactat
     5221 gcactgggcg gtgatttcaa cagccgttta aacctgaatc tcagagaaga caaaggctgg
     5281 acatatggtg cccgcagctc ctttacggct gatgaataca ctggtgattt cgaattcagt
     5341 tccggtatcc gtgccgatgc aacagacagt gcattgattg aagtaatgaa agagttgaaa
     5401 aattatcgtg ataacggtat caccgaagct gaactgaagt ttatgaagaa ttccttgggt
     5461 cagcgggatg cattgctcta cgaaaccggt attcagaaag caggctttat cggccgcata
     5521 ctggattata acctgccggc caattatgtg gaccagcaaa acaagatact cgccaacctg
     5581 accaaacagc aggtagatgc gatttcgaaa aaaatgctga accctgaaaa aatgaatatc
     5641 ctgttggtag gcgacaaagc caaaatcata gagggagtga agaaaacagg ttatgaagta
     5701 attgaactgg atgtagatgg aaagcctgta cagaaaaaag ctttctaagc agagtaacta
     5761 ttaaagcaaa accgtcacca ggtggtgacg gtttttttat gccatgttta taccggccag
     5821 tgggtatttt ttaacttttt aacaatctaa gccatgaacc tttgcggtat ttgttgttat
     5881 ttaaaggaca aaaaccttgt aatatgaagc ttatctacac tcttcttttt cttactatca
     5941 gtattttctc ttctgcccag ttttacctga gtagtggtta ttcgctcagt ttaccgaagc
     6001 aggaaatgaa aaaaaatatc agggctgttc atagccttgc cctgagtgga ctatatcgtt
     6061 taccgggtaa catggaccgg gtacaattag gtgtcgattt tggctggggc atgtatgcca
     6121 gtacaagaaa ggaacaaaca tttactttcg caaacggcga cattacccgg actgatgttt
     6181 tttacagcag catggtggtg cagggtggcc tgcaaacaag aatagacctg ctgcgaaata
     6241 aaattgttac accttacctg agcggaaaaa caggctacac aagtttttat agtaacattt
     6301 ttgttgaaga ccctcacgac ccgggaggat gtgcgcccct ggaccagcga aacattatta
     6361 aagacggaac gatctatacc ggttacggag gaggtttgca agttgactgg agtatcttca
     6421 atagaaatag cagaaaaagc aggggatgga ttgatctgac tgtcaataac attcagggag
     6481 gcaacctcaa ttatatcaac acaaagaaat tggtggatgc caataaccct cccgttaact
     6541 ctgatggaaa gcctttaaat atcactttta taaatgcatc cactcagcaa ttgcatgagc
     6601 accaggtggc tgaagtatat acaagccctt tgaggatgct ggaattaaaa atcagcgcta
     6661 catttctttt aaactaaaaa ttccacagca taaaaagtaa agcggctcca accacagagc
     6721 cgctttttta taaccaaaac caaccacaca tttcagacaa tctttaagcc ttttttgctg
     6781 ctggcttagg cttgcccagc catacttttt taccctgaat tcttcttacc aggtacatga
     6841 taccgacaaa tatgaagaag aagggtccgg caaacccaag cagggcaata tatttggtac
     6901 catagaattg ttgcatgggt cttttcagcc tttctgcaat aaggtacata ctcggcacca
     6961 tcaccaaggt caggaagaaa gagaatatca gaccaaatat gatcgtccat gctaatggcc
     7021 cccagaaaac aacactatca ccaccaaaga acaggtttgg acgaagatgc tggaataatc
     7081 cggcaaaatc aatattcaat ccaacagcca gcgggaacaa acctaatatt gttgctactg
     7141 ctgtcagcaa tacgggaatg atacggatct ttcctgcctg tatggctgct tcacgggtac
     7201 gcataccacg gctacgtaat tcatcggtaa actcaatcaa gaggataccg ttcttgatta
     7261 ctataccggc aagaccaata atacctactc ccatcatgat agtcgctacg gtcattccgg
     7321 taatggcata tcccaggaac acaccaataa tagagaagaa tatttccgtc aaaacaatga
     7381 atggcttgct catactgttg aactgcaaca ccagtatgaa gaagataagt cccaatgcta
     7441 tcaataatgc tttacccagg aaagcggacg tttccttttc ctgttcactt tgtccgctca
     7501 gtttgatgtt gacatctgca ggcactgcat ttttttcttt gaactctttg atcttagttt
     7561 ccagctgcgc attgattggt gctaccatcg ttgggtcaag tacattactt tgtaattgaa
     7621 tagtgcgttt cacattctta cggttaatgc cgccgggaga atttgtatag tcaacagaag
     7681 ccactgaact gagcggtaat gatttcacct gcatggtatt catgtccatg aaagtgatcc
     7741 gcatgttcaa caggtctgaa acatttttgc gcaggtcttc tttgaaacgc aactgaattt
     7801 tatactcatc ctcaccgtct ttgatcttgc tcacttcttt accaaagatg gctgtacgca
     7861 gttccatgcc gatttgaacg gtgctcaggc cctccatagt agccctttgc ctgtctactt
     7921 taacagtgat ctccgggtta aacaggtcaa catccggttg caggttctca ataccttcta
     7981 cacgattggt gtctaaaaag cgatacaggt cagtagcaat ctttgttaca ttttccaaat
     8041 aatctcccga cacttcaatg ttaaccggtg gatcagtagg cggaccatta tcttcctggg
     8101 ccacttccac actggcaccg gggataccct gcaccacttc gcggatctta tccatatacg
     8161 gcctggtttc cttgccatgt cttttttcaa actctacaaa agaaacctgt atccgtccaa
     8221 gatgcgattg tacactcctg ttgttgtccc tgggattgtt ggcatttacg gccacattgg
     8281 taatgatgct ttcgacaata ccaccgggag ttccgggttt ttctttctcc agcacttttt
     8341 ctacccggtg ctccagtata tgcaatacac tgtctgtgtg acgaacatct gtaccaaccg
     8401 gcattttcag gtatacataa acaaagttgg gatcgccgct cgggaagaat gttgatttcg
     8461 ttcttcctgt tgtaatactg acgaccagca tcagcaatga aataataaac aggccaaaca
     8521 gtgatacgaa agcccatacc ggcctctttc ctttcagtat ccatctcagc aatcgctcat
     8581 acctgctcat cagccttggc agaaaactat tctgaaactt atggatcaca tcgttcaaca
     8641 cataagcatt gaacaccata aggattgcca gtaacaacag gaagttggct actccatgga
     8701 agcctggtaa atgaagcagt ataccgccta taagtgatgt aaggaaccat ttattcctga
     8761 aaacggcttt tttgggtttt tcaaattctt tcccctcggg tttcataaaa ctcactgcaa
     8821 acacagggtt gaaaaagaat gccaccacca atgacgccgc cagtgtcagg atgagtacaa
     8881 cgggcagata tatcataaac ttcccaatca ggcccttcca gagcaataga gggaaaaatg
     8941 gtgccaatgt agtggccgta ccggctaata ccggaatgaa aacctnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca atgcaaacaa tacaatgaag
     9121 ttgagagtga tattgatgcc agagatactg ctgaccagcg gtatgaacaa gaaggctaca
     9181 aacactgaca gggggacact cagcgccaca aagaaggcat tggtaacacc cataaaaaac
     9241 atcaggatga tgagtaccaa caagaacccg atgatgattg tgttgatcag ttcagtgaat
     9301 gaggtttttg tttgtttact tgtatcgccg gtaaattcaa ccctgagatt tttaggcaat
     9361 tcgcctgatt tctgcatgct ttccacctca gcctttactt tatcagcaca gtctatcagg
     9421 ttttcaccag aacgcttaac aatatttagt gttaccacat tctttccatc cagccgggca
     9481 aaactttcct tttccttcac tgtatctttt aattctgcca catctctcaa ataaacagaa
     9541 gctccctgtg cactgctgcg tacgattaca ttgtataaat catttacact ggtaaattgt
     9601 ccttttactt tcagtgttcg cttcatatta cccgtttcta tcaaaccacc tgtgatatca
     9661 tggttctccc tgctgatggc attttctaca tccatcaggc taaccctagc cgctgtaagt
     9721 ttgtaaggat ctacatttac ttgtatctct ctttccggtg caccaacaat ttctgctctg
     9781 ttcacttcac ccaggctttc aaacctgtcc tgcattttgt cagcataccg cttcagctgc
     9841 ataccatcat aatcaccact gatgtttaca aacatgatgg gaatttcact aaaagcaaat
     9901 tcctggacat tgggttcaga agtcagatcg tttggaagat ctgacatagc cctgtcgatc
     9961 gcatccttta ctttttgctt ggctatgtct gtttccacat cggtatcaaa ctccactaca
    10021 ataaggctat aatctgcctg tgaaatgctc tgtatcttat ttaccttggc gccgctgata
    10081 ccctttaatt ccttttcgat gggttgtgtt accagacttt caatatcctt ggctgagttt
    10141 cctgcataga tggtggtgac agaaattgtc ggcacaacaa tatccggaaa ctgctccttg
    10201 ggcaacgtaa tgaactggag aaaaccaata gcggagatga taatagccag gatnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagacta
    10381 cttaccggta gtgaccgcct ggccatcata gatagactga tacccttctg taataagtaa
    10441 ttcgccaccg cttaagccat ttttaatttc aataactccg ttgtatactt ctcccacatt
    10501 tacatttttc tttcgggcta tcattttatc ccctgatttc tccattacgt atacatattt
    10561 tccattttca tcactttgta caacattcac cggcacggca atggttccct ttgcttcgta
    10621 gtcaagtatt ttcattactg ctgtttgatt aggcttcagt aatggatcag aaggcagttt
    10681 agcttctgtc atgaatgaac gggtcttttc atcaatagac gcccccacaa cactgataac
    10741 tgattcaaaa gcaggtttgc ctgtttccgg aacagaaata agcactttat ctcctttttt
    10801 cactttagcc acatagttat ccggcaccgg cacttcggct ttgagtgtgg tggcatttac
    10861 aattacgatc tgccccccct gctgtgtagc accactaaag aattcaccca ctcgtacatt
    10921 aatttcttct accacaccgc tcattccggc agtaacactg gtcatattca aagcttctct
    10981 ggcctggcct acctgtgctt ctgctgctct caattgtgca ctgatggcat ccacatctgc
    11041 ttttgcattg attacattta tctccgcacc tatattctgc ttccagagat tctggtaacg
    11101 ttcgtaaatc gtttgtgcct gtgccagtct cgcttttaac tgaccggttg attgctgcgc
    11161 agcaatcaag gcctgttgct gaagggcatc atccagcttt actaccacct ggcctgcacc
    11221 tactttctgt cccactttta cataaatggc ccttactact ccgccaccac ctttaggcgc
    11281 tacataaccg ataccgctgt tggatacctt tccctgcaaa tcaatgtaat gagcaaaatc
    11341 ctggacacgg agtgtatcta tcgccacgag tctttttacc tgctgcgcag cttcaggatc
    11401 tgccttggct atctcgtcct ccatcttttt gatatccgca tcaatttcac ttttttgttt
    11461 tttcagtttt tccagcctgg atttaaggtc accaaggttt cccttttttt ctttggctcc
    11521 accgccgcag gctgccagta cagctgcgaa aaaaacagct gccgatattt tcattatatt
    11581 attcatacta tagtttttat tggtcatggt tgttgattta taattttccg gttgctttca
    11641 tataatcgat cttagaaatg atcgcatcat acagagcagt tatataactt gtttgtgccg
    11701 ttttcaattc tacctgcgct gcgttaattt ctgtttgcga acctgttcct acttcgtatt
    11761 tcttctttgt ctgctggtaa actttttctg cgagttccat attttttttc tggaaatcca
    11821 ttgtggctat ggctgaacgg aaattattct ttgccgtctc aacttcatta tcgatcgtca
    11881 gctttaatgc ttcaatctgg ttttctgtct ggcgcagctg tattttagtc tgctgaattt
    11941 ttgattttgt ataaaacccg ttgaaaatgg gaatatttat attcaggcta atattggaaa
    12001 tggtaaacca gtcacctttt ccaaaaaaat tccatttgtt tcgttgtgca ttcttggcgt
    12061 actggccatt gagcaataca gttggtatct ggctcagttt gtaccggcgg atattatact
    12121 cattaagttc tttgcctatc tgggcatatt gaaattcctt cctgtcctca tatttataga
    12181 tactattttc taatattcct tcttttatct gttcatcggt aagcttgtca gttagcacca
    12241 gttcatccct taccggcata cccatcagca ctttcagtcc atagtatcca ttggagatac
    12301 tgttcaatac tttttgcttt tcagtctgca ggttggtcaa ttgtacgttc agtttatcta
    12361 catccagctt ttcagcaaaa ccgttttcat aaatgatccg ggtatccttt agcagttttt
    12421 caagcctttc aatattagcg tccagcaact ctacctgtgt cttgctaacc accagctggt
    12481 agtacacctt atgaatgttc gccttgatca tttcctctgt tacttctact ttctttctgg
    12541 caaaacgcat agtagcatct cttgccatca agccaacaaa cacctggcca tcaaacaaaa
    12601 tttgtgtcag gctgatagct cccactgcac tgtattttgt tccaaactgt gcagcaataa
    12661 atccaaaatc acctggcatc tgaatggggt taccattacc atcccttact ccctcatcca
    12721 ccagtacctg gtaagtagag ggtgaaataa agttggggat tacctgtgtg gcaacattgg
    12781 gattgtaagt tgttccaagg cttgcattga ttttggggta agcattggaa gtcacttccc
    12841 tgttaacctg ctcctggtac aacacatctt ccagcgcatt ctttacattc acattattct
    12901 ttaaggcata atcaaccgcc tgctgaactg aaaattcatg ctttgtaatt tttacagaat
    12961 cgctgtttcc accaccctgt gcccgggcca taccagaaat ggagagggca aggataaaaa
    13021 aaatgaaagt tggttttctg aaggtcatca tggttaattt ttttgacgtt gttctttata
    13081 tttctgaatc atcctatggc cttttaaagt agctacacca tatataaaca actccaatat
    13141 catattggtc gtttctgcta cattatattt accaggtgga aacactgcca cattaaaagg
    13201 cagcatcatg gattccatcc tgtattttga aataatatca atattcaggt ctgcacggta
    13261 caactcatcc tttatacccc attcaaggtt attacgtaca atactttgca gataactgtc
    13321 tttgtgttcc ctgaatttct ggtaggcttt aaaatgaaat ttttcaaggt cgtataatac
    13381 cattgggttc atgttgctga attcttccat cagcaattcc atggtattaa aaatttcatg
    13441 aatagcatct tttgcattgc ttttacaatt accgcaatcc tgcttcattt gaacgatatg
    13501 caagtccacc actgcttcta ccagcgcatc cttatcagca aaatgctggt acaatgtttt
    13561 tttactcata cccagcccgt tggcaatatc atccatggaa acagaacgga taccgtactg
    13621 catatacagc tcattcgcct tttcaattat tctgtcacga acttccatta cgaaatttga
    13681 gggagcaaaa ctatggaaac ttttttcgtt accaaagttt ccatcatctt tatttaccat
    13741 ttatcatgta tagtgatgag tgctccttgc caacaacgtc ggggaatgta agtcagcaac
    13801 catcgctttt taatggttta caatgattta aaaggtgata gtggtttatc gtacttttat
    13861 tgcatgaaca atcgtttttc cctgcgttcc tatttgccaa aagaaccttt ccggtttaag
    13921 aaagtagttc aatatttcct gcaggggtta ttgatcattg ctccgctggc tataactatc
    13981 tatgccattt attgggttgt atccacagtg gataactgga ttcctatttt ccgtgagccg
    14041 attaaagatg cacagggcag gattatcggc cacgaagtaa aaaattatgg gttgggattt
    14101 gtagtcatca tcacagcggt cattttaatc ggttacctga gttccttttt tattcaatca
    14161 agattattca acctgtttga caggtggctg gaaaaaacac ccggtataaa atttatctac
    14221 accagcacca gggatttttt tgaagctttt gcaggtaata aaagaaagtt caataaagcc
    14281 gttctggcca atgtattttc ggatgaagtc tggattgtcg gttttcttac ggatgaagag
    14341 atggcaaaat ttgatatggg tgcagataaa gtagcagtat atgttccgca ggcctacaat
    14401 tttgcgggtc agctatatat tcttccgaga gataaagtaa aaaagataga acacattacc
    14461 tccggcgagg caatgaagta tgctgtaaca ggtggtgtgg tagatctcga agcagaggat
    14521 gcagatcagg aacaaaaaca ttaatttcat accagcttag ccttatcttt tgaatagttt
    14581 gcatgaagaa atcgttgcta cttatcatta ctattctgtt ttccatcaaa agcttttgct
    14641 ggggcttcta cggccatcga aagatcaact atcatgcggt atttcttctt cctcctgaaa
    14701 tgatggtttt attcaaacct caaattgatt tcctgtctga acatgcagta gatcctgata
    14761 aaagaagata tgccgtaccc gaagaaggcc caaggcacta tattgatatt gaccgttatg
    14821 gccattatcc ctacccggat ttaccaagaa aatgggagga tgcggtagca aaatattctg
    14881 aagatacgct taatacttat ggaattgtgc catggtggct gcagacaatg ctctatcggt
    14941 taaccagcgc atttaaagaa aagaaccagg taaagatatt gcgatatgct gcggaaatcg
    15001 ggcattacat agctgatgca catgtaccat tgcatgccaa cagcaaccac aacgggcagt
    15061 tctccggcca aaagggaata cacggttttt gggaaagccg tgtaccggag ttactggcgg
    15121 aaaaagaatg ggatttcttt atagggaaag ctgaatactt aaaaaatccc gctgatttta
    15181 tctggaaaag agtattggaa agtgctgctg ctgcagacac agtacttcaa atggaaaaag
    15241 aactgacagc cagttttccg caggatcaaa aattttcatt cgaagataga aacggaatta
    15301 ccatccggca gtattcatct gccttttcaa aagcatacga cacccggcta aaaggaatga
    15361 tagaaagaag aatgaggcag tctatacatg ccactgcttc tttttggtat accgcatggg
    15421 ttaatgccgg tcagcctgat ctgaaaaaac tgaataacag ggaatttact aatgaagaac
    15481 tgaaagaatt cgaagtttta aataattcct ggaaaaatgc acccatcaaa ggcagggaac
    15541 atgaataata actgatattt tactgcacaa tcgattgtgc acccttggta tttacgggtt
    15601 ttattgtcta cttttatcaa ctgtttttac ctatgtacaa acctccatgt atgaaaaaaa
    15661 atttactgct tgcacttgtc tgcatgtctt ttgttatctc caatgcccag atttctgttt
    15721 atgaactgat ggaaagaaac gatatctcca ttcaggaggc tgagagaata gctaatcgtc
    15781 attttgatac agctggtaca ggaagaggaa ccggctacaa gcaatttcag cgctggttat
    15841 atgagcgaaa gtttcatata gatgaaaacg gaaactacct gtctcctcaa acagaatgga
    15901 atcgctatac agaaagccgg actgctatgg gatctttcag aaccgaagca ggtagctgga
    15961 cagaattggg cccatggggc tggaaccgta ccagcggatg gaaccccgga acaggaagat
    16021 tgtctgcttt cgccattcat ccggccaacg agaatattat ctatgtaggc tctcccggtg
    16081 gaggtatctg gaagtctgta aacggagggt tgaattgggc ccctcttact gataataacg
    16141 ccaactggat gtctgttttt gctttaacca ttgatccgtt aaatcaaaac attgtttatg
    16201 caggtatgag cggaagcgtt ggcatcatca aatcaactga tgccggcgca acatgggctg
    16261 ccgccggagc agggccgtca ggcaccgttc ggaagatatt aattcatcca acaaacagtt
    16321 caattgtttt tgctactgca acaaacggaa tctggagatc gataaatgcg ggagcatcat
    16381 ggacccaggt acatagtggc agcaaagaag atattgagtt caaaccagat gatgtcaata
    16441 ttatgtatgc aaccggtaat gatgtctatc gttctgcaga taatggaata acatggacac
    16501 cagtatcagc agcacaaggt attaccaata ccggccgcac cttagtatcg gttacaccag
    16561 ctaatcccaa ctatgtatat attgcccagg ccagcggaag cctattcggg agaatgtata
    16621 aatctactga tgcgggactg accttcacta ctacagttgt aggtagcccc gcaagcggca
    16681 ctaactattt tggctacgaa accaatggaa ccggcacttc tggccaggct acttatgata
    16741 tggcgatgga tgtttctccg acaaatgcgg ctgaagtgca tattgcaggt atcatttgct
    16801 ggaaatcatt agacgaagca acttcattca cggctgtaac tgcatggtca ctccccaaca
    16861 gtatcggata taaccatgcc gatgtacatg gtttattctt catcaactcg aatctttatt
    16921 ccatatcaga cggaggtctt tataagagta ctaataacgg agaagactgg acggaccttt
    16981 ccactggttt gggtattcgt caattttacc gcattgccag ttctcaaacc aatgcgacgg
    17041 taataacagg aggtgcacag gataatggta ctgccgccaa acaagcctct ggcacttggg
    17101 tggattggct cggtgccgat ggtatggaag gcctggtaag ccctaccaat catttaaacc
    17161 tctggggcac cagtcaaaac ggatcaatct accggtcaac aaacggagga aattcttata
    17221 gcgggttgcc gaggccttcg cccggccaat gggtaacacc tctggcaata catcctacca
    17281 atgaaacaat tttatatggc ggctggacag gcgtatataa atctaccaac agtggcagca
    17341 gctggacaaa tatttcttcg ggagtaatta caactacatt agctgattta gctgtagcac
    17401 cctcagatcc aaattatatt tatgcttcaa atgggtcgac actttatgtt acaacaaatg
    17461 atggcgccac ctggactacc cgcagtgctc ctgccacgat caacgacata gctgtagatc
    17521 caaccaatcc atcgaaaata tggattgcgt gcaacagcac cactaacagg gtgatggttt
    17581 ctactgatgc cggagccact tttacaaatg tgtcagcaaa tttacccgcc attgttgccc
    17641 gtgctgttgt tgttgacgat aatacccccc ggggaattta tgtaggaatg aatattggtg
    17701 tttactacaa aactgaacct gacgctaact ggacgaatta ttctgataac cttccgctcg
    17761 ttgcaattaa tgaattggaa attcaaaaga atgccggtaa aatacgggta gcaacttatg
    17821 gccgtggtgt ctgggaaagt ccaatggctg ccgttccggg tggatttgta tttggctctt
    17881 ccacaccagc tactgcaaca tgcccggctc cctcagtaat gaatgttact ttgccaacca
    17941 gttcggtcgg aagctttgtt aacccgatca gtttatcagc tacagccgga gtacctggcg
    18001 gtactaccgt taccttctct cctaaccctg taacacccgg cagcagtacg attgtttcac
    18061 tcaacaatgc aaatacactt tcagccggaa catacactat cactgtaacc ggaactgcta
    18121 ccggagctac gacccaggta actaacgtta catttactat caacgccgga agcggaccag
    18181 caataactgc acagccggct gatcaaacag tttgtagcgg aacaaataca tcattttctg
    18241 ttaccgctac cggtgctacc ggttatcaat ggcagttgag tatagatggc ggcgctacat
    18301 ttaataatat ttcaaatggc ggagtttact caaatgccac aactcctaca ctgaacctta
    18361 caggagttac agcgggtatg aacaattacc gctatcgttg tattgcttca actttatgcg
    18421 gaagtacgac atcagctgcg gcaacattaa ctgttaattc agctccggca ataactacgc
    18481 agcctcaaga tgtattgttg tgtaccggaa gcaaccatac tttcagtatt acagcaaccg
    18541 gcagcggctt atcttatcaa tggcaactga gtacagatgg tggtgtaact tataataata
    18601 tcagcggcgc aaatacagcg agttattcat taacaggaat tacacctggt atgaatggta
    18661 accggtatcg ttgtgtagtg acaggatcct gttcacctgc tgccaattca aacggtgcaa
    18721 ctttatcggt tgtaacttct gtaactgtaa caacacagcc tgctaacagt accgtatgtg
    18781 caggcgccaa tacaagcttt acagtagcag gaagtggttc aggtattatt taccaatggc
    18841 aggtaaatac aggcagtggt ttcaatgata tcactaacgg cggtgtatat tcaggagcta
    18901 ctactgcaac actaaatatc acaggcgcta ccaccggtat gagcggatac ca
//
LOCUS       DAORLS010000020        18876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1568_length_18876_cov_2.149886, whole genome shotgun sequence.
ACCESSION   DAORLS010000020 DAORLS010000000
VERSION     DAORLS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18876
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_18876_cov_2.149886"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK977_03050"
     CDS             <1..295
                     /locus_tag="PK977_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF17111.1"
                     /translation="RSDMQEQQLQAGVKPSLVNWCPAPAAPDGLLHTEINGEVVRKET
                     TSSPGNYMGYYDDVYKALTTQMPNPVPAADGINNMRIIEAALESFASGKIVSF"
     gene            306..1676
                     /locus_tag="PK977_03055"
     CDS             306..1676
                     /locus_tag="PK977_03055"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRF17112.1"
                     /translation="MSYEFSIIGKKRLFKFIKQFIILNSSFIIFLSSCSVSKQIAKSA
                     KEEVLGNKALLAAHVGISIYDPADNKYLYNYQGDKYFVPASNTKIPTCYAAMKYLGDS
                     LVGFMIREVSDTIWLKPCGDPTFLNSEFTNQRIFNYLKGTSKNIYVVFHSKDEIQEYG
                     SGWSWNDYEEAYMAERSAMPVYANVISFEIKDNRLLVIPPILKRPFFNEPMIQLMGLG
                     DKFSVIRDKDENKFRFWSATTSFSKDRIPFKTKNGITNFDFLRDTLNRSKDKLGFGLS
                     LQFDEYKKNHSQPTDSLLKPMMHRSDNFFAEQSLLMVSNEKLGVMNDAKIIDTILKTD
                     FKDLPQKPRWADGSGLSRYNLFTPQSFVAILNKMKNEFGMERIKVIFPTGGEGTISSY
                     YKADSGYIYAKTGTLSGVVAFSGYLYTKKRKLLIFSTLINNHQSSSTDIRRAIEKFLQ
                     GIRNKY"
     gene            complement(1788..>2020)
                     /locus_tag="PK977_03060"
     CDS             complement(1788..>2020)
                     /locus_tag="PK977_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HRF17113.1"
                     /translation="EQYKHFSPEVKFSKREKLVRSLARQQAIKTGTRLTEREMRQLVN
                     DLFACEQPNTTPDGNPAYLEFKQEQLERMFGK"
     assembly_gap    2021..2052
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2053..>3197)
                     /gene="mutL"
                     /locus_tag="PK977_03065"
     CDS             complement(<2053..>3197)
                     /gene="mutL"
                     /locus_tag="PK977_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRF17114.1"
                     /translation="GTSIAMKNLFFNVPARRNFLKSNAAEMRHIVDEFTRVAMSFPQI
                     LFTLTANNQQLFHLEAGSLKQRIVQLLGNNYNAKLVTVKEETDYLNVYGFVGKPETAK
                     KTRGDQYFFVNNRFIKSPYLNHAVMSAYQEMIPADSFPMYVLFIELDPTVVDVNVHPT
                     KQEIKFEDEKIIYAFVQAAIKHALAQFSVTPTLDFDLDASIQQLPSIQQPFTEEKRSA
                     AASGSIFKGFTQKNQAHFIEKSERSELKNWRDFYESREPGIGSQESAPVSQAPAHYSP
                     FTIHQDTELTQLLNTYIAAPSKNGFLLIHQQAAHERVLYEQLKTASKDKPVATQRSMF
                     PSTLELTPADAAVMEEIIGDLQYLGYVIEPFGKNSYVIQGTPADVEAG"
     assembly_gap    3198..3207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3208..3626)
                     /gene="mutL"
                     /locus_tag="PK977_03070"
     CDS             complement(<3208..3626)
                     /gene="mutL"
                     /locus_tag="PK977_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRF17115.1"
                     /translation="MPDKINLLPDNIANQIAAGEVIQRPASAVKELLENAVDAGATEI
                     KLIIADAGKSLIQVIDNGGGMSETDARMCFERHATSKIKDIEDLFHIKTMGFRGEALA
                     SIAAVAQVELKTKRAEDETGTYIEVENSVVKKQEPVAA"
     gene            complement(3701..>4334)
                     /locus_tag="PK977_03075"
     CDS             complement(3701..>4334)
                     /locus_tag="PK977_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17116.1"
                     /translation="LPEPIDASDKQIKFQALSMLRSLYRDYWVDKGPCDCTTKAIALA
                     NNTNTAASDVLGGLAIGIIFAPIESLPGLSFAAKLGIGAAKALGSALYGGESFTDELA
                     KNLFDVIGGEIFPGLLDSETAGNAANKFAGKGLEELMKAEGIRSTQWEGETTLRECGK
                     VKGKTTMLFNPNTGWVTLLIKIDNCPLIVIKYKVNKDGVPITKPSVTKVQ"
     assembly_gap    4335..4353
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4354..6889)
                     /locus_tag="PK977_03080"
     CDS             complement(<4354..6889)
                     /locus_tag="PK977_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17117.1"
                     /translation="MKPFTIHLKIFTCLLLISQLAAKAQIINSEKGLTTAEFILSEGK
                     IKIYLPEDIRQGDHISGTVVMEAAGNTEKEKSRNIQSLQKYQLGHADGSFGKQGLSGL
                     FNYTLKGPNTNFTLSANGKIISSLMIPLKSNIATRTNLSIPTHALTASPLPMKGQFDG
                     NAENTKCMIDGRHIEILAESPRQCIVQLPQVTSGAHNILISENNKTTEQKICAVNLDV
                     NAGRLNLQKGESTYIDVIITGLQNLPSAATLSVVNTSTATVMMTGGDAQVIHIQPATV
                     SVAGSFTKRFDLKSVKTGNFSVNVNLDLPEPASATGNAASLCNCYINEQSCLIPLQTC
                     LALGGSTNPPVFYNTSPDNQNPKISNPPPAILLSPGNINAQTYQVTLQVNVLNDDVAA
                     VIFSEKPVSGTEWQQAGRASFNGASWSANWLPPLGNDGQHIIRARVAGKNSTITELYT
                     GVYLQLTPATIKTMPGERVNFTVTDAQLSNANQNVQQQVENLWRIQEKLARLRRKLEE
                     LIEEMDRNKTMADELAAIDKTIEKIPGLYADSLKKLTDSLAKLRAALPPNPDPADLQK
                     TADEASQRAKDCEDRLNKLKQEKENAQQELNNLNNEIENLLKQLDALHLGNNWSGGHG
                     YHKDGSFWYGYVGDENSNTNIWSESNAISSKLKALRKPQNAAKNRLKALEAEITKAQE
                     ECDKLKKEKDKAEDAAKKGNAMAAAETQIDELCRQIQALLDALKKWCAAHPGVCHFNP
                     ELPGKPRTVADLEAFLSQLDDIINKKKQKEADLEKTADEKASEAQGVGNDIKAAEEEM
                     KAAEEEMKKAQAAADKLQAEREKQLEEERAKKRKEEEEQAKNSAPKP"
     gene            7010..9934
                     /locus_tag="PK977_03085"
     CDS             7010..9934
                     /locus_tag="PK977_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF17118.1"
                     /translation="MKKLVMQRLQRLVFLLVFLPLFSNGQYKSKLGKQQWVDSVYSSL
                     SDDEKIAQLMVVRAHSNLGPDHVAKVVRDIQKYNVGALCFFQGGPVRQANLTNYYQSI
                     AKTPLMVTIDGEWGLGMRLDSVTKFPYQLTLGALDDAELVYKMGVAVGQQCKRIGVHV
                     NYAPVVDVNNNPNNPVIGYRSFGEDKNRVSALGVAYMGGMQDAGIMACAKHFPGHGDV
                     DVDSHYDLPVINKTREQLNEMELAPFQSIFKAGVKSVMIAHLYIPSIDNTANKATSLS
                     KNNVTDLLRKEMGYEGLTFTDALEMKGVSKFFPGGTIVVEALVAGNDMLCLPENVGDA
                     IAAVKKAIAEGKLSWNDIYVKTRKVLNEKYDLGLFEVWPINTNNLLNDLNAKTDAIRK
                     EVAEKTITLLSQAAFKASRTDYAGIPLSKKEKIAYVGIGSTELNTFGKRMKDDFGADV
                     FLFSYKDGKEKAQEILKQVPKDGKYDAIVIGVHDYSLRPTNNFGISQAAIDLYKELNF
                     IKTVTLTFGNVLATKNFCDAWTLAACYQDDPVTQNAAADLLLGKFKARGKLPVTVCSF
                     KPGDGIVAAKTAVEEMQAKLKVIDSIAQDGISKKAFPGCVVLAAHKGEIIYHKAFGNY
                     EYEPSSPKMAAESIFDLASVTKVSATTVSIMKLYEEGKIKLDGKLKDYLPYTKNTDKE
                     DLKIDEILLHQAGLVPFIAFYKETIDSSGAPKPAVYAAKPQKGYGVRVAENVYLRNDW
                     QDTMMMRILKSPLGPKNKYVYSDNDFIFLGKIVEEVTGLTLDQYVQKTFYNKIGMYST
                     GFKPRNRFQLECLVPTETEKHFRQQTTRGDVHDEGASMFGGVAGHAGLFSNAYDLSLL
                     YQMLLNGGELNGERYLKPETIKLFTSYNSNISRRGFGFDKPEKDNATRKEPYPSALAS
                     PETFGHTGFTGTCVWVDPKYDLVYVFLSNRVYPTRDNGKLSQLGIRGKIQDALIKAVS
                     GQ"
     gene            9960..10652
                     /locus_tag="PK977_03090"
     CDS             9960..10652
                     /locus_tag="PK977_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17119.1"
                     /translation="MKPFLTVILLFTSLLFYGQHTPNEFKKFRIASAAETCSFKEDGV
                     PQKFTYTAYYDRNGNDTAYYHDELCIYRKIYTLDENNRIAMLIMYDKDGKEREKTEYT
                     YEANGDYSTKSYHKLFDAVTIKWFDKTDRLLKEQRDEETTLIYDYNSKGQLIQVKTKP
                     GPNSKGEIEHLKYTYNNKGQCIKEQSLGTYPWVKTLFYDAKGMLTKTITTGKEAGVAY
                     TNTNTYQYTFWK"
     gene            complement(10653..11990)
                     /gene="ricT"
                     /locus_tag="PK977_03095"
     CDS             complement(10653..11990)
                     /gene="ricT"
                     /locus_tag="PK977_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRF17120.1"
                     /translation="MNAYDWLHNLPMADFDSACKVIEVSFSQGTRKDFFRNPMLQPFE
                     KGDLIAVEGVSGFDIGEVSLTGEIVRLQMKKRGVKEDNPEMKKVLRRATDRDIEIWKQ
                     NKAREPEAVIRSRAIAKQLKLDMKISQVEMQADGRKATFFYIADGRVDFRELIKVYAS
                     EFKVKVEMRQIGARQEAGKVGGIGSCGRELCCSTWLTDFKSVNTAAARYQNLSINQTK
                     LSGQCGRLKCCLNYELDTYLDALQHFPDNCDVIQVAKGNAFLIKKDIFKNLMWYTLPD
                     SNKQYPLTIERVRKIKSLNQQGITPDELEPVEVTTGKPKEAEPETVELVGQISLRTLE
                     KAEKKKKQQQQQRNQPNRQSGQRPVLPGCLRRRAVRRGRGELPPDRQSGQRPQQRQQG
                     GQQQGGGQQQRRQQGGQQKGQQRNPNRNQQRPGNPNRNQQRPDNPNQGKDKQV"
     gene            complement(12346..>13204)
                     /locus_tag="PK977_03100"
     CDS             complement(12346..>13204)
                     /locus_tag="PK977_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17121.1"
                     /translation="IATDFITEWREFIKLNPYGNLFDWIELIKEKENSQGKITSRECD
                     EIIKKLSLKSFESEYKVLILWMPEELEKEGNKLLKIIEEPPANTLFILVAENEELILP
                     TIVSRCQLIKIPALENKEIEEALISRNKTEASIARQVASVSEGNYREALQLVQHAEED
                     WQSLLREWLNAMLKTGPVAQAKWVEEISRLGREKQKQFLRYFNHLLEQAILLKATGNT
                     PGAFGTERDFAERLNKIAGIEQQQAIIEEIDKASYYIERNANGKMLFHALTIKLYHII
                     QDKIVFLMD"
     assembly_gap    13205..13282
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13283..13570)
                     /locus_tag="PK977_03105"
     CDS             complement(<13283..13570)
                     /locus_tag="PK977_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17122.1"
                     /translation="MTFSDIIGQKDVKQQLAELVHHNRLSHALLFLGKEGSGALPLAL
                     AFAQYVTCEKVQGKHTAIQAGPSLFGDPEPVTVNNQPVTDSCGACPACQKAQ"
     gene            complement(13652..14167)
                     /locus_tag="PK977_03110"
     CDS             complement(13652..14167)
                     /locus_tag="PK977_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRF17123.1"
                     /translation="MKSLLLTLILSATLTGGIYDFKVAGLDGKEIDFSKFKGKKILIV
                     NTASKCGLTPQYEGLEELYKKYKDKLVIVGFPANNFAGQEPLNNSEIKDFCKQNYGVT
                     FPMAEKISVKGDDTHALYTWLKSQAKEKGFTDPVTWNFGKFLVNEKGELIATFSPRTT
                     PMSEEILKYLN"
     gene            complement(14240..>15008)
                     /locus_tag="PK977_03115"
     CDS             complement(14240..>15008)
                     /locus_tag="PK977_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRF17124.1"
                     /translation="KDTADRVYFTGDRLDEPYKDFPANWPGIFFQAGSKDNVLNYAVI
                     RNAFQAIGITDPSPNASPKLILNECVIDNNYDAGIVAVNSSIRARNCLISNCGKNLFL
                     VKGGDYQFTHCTIVTIANRYVDHKDPVLALTNFTNNSTADLNAIFRNCIFWGQNGLVD
                     DEVVVLKSGNTVFNVNFDYNLWKVQAAPAGVSANQIINNQTPQFDSINTSRNFYDFRL
                     KSTSPAVNKGTSTSVTTDLDGNPRAVGLPDLGCFEKQ"
     assembly_gap    15009..15087
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15088..15633)
                     /locus_tag="PK977_03120"
     CDS             complement(<15088..15633)
                     /locus_tag="PK977_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17125.1"
                     /translation="MKIFGLLTISFLLLFVSCRKDSFITSPDARVTISADTLKYDTVF
                     VTAGSTYRTFKIINENNQKLLISSLQLSGGTSSAFKMNVDGTAGTRFNNLEINANDSL
                     YVFVQVNVNPGAANLPFVIRDSIQVSYNGKDRLVQLEAWGQNANFLRNKVVSTNEIWN
                     NNLPYVILGSLTINTNQTLTIN"
     gene            complement(15691..16389)
                     /locus_tag="PK977_03125"
     CDS             complement(15691..16389)
                     /locus_tag="PK977_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRF17126.1"
                     /translation="MTLLREIKESVLHLLFPHVCTGCGSDLLSKDSALCMRCVEAMPE
                     TQFELHAGNPAEKTFWGRLPVAAASAQFYFTKESLMQHLMHQLKYKGNKELGIQLGRI
                     MGEQLKNSGRFPVDALIPLPLFPAKERRRGYNQATVLCHGMAETMHVPVLNTVITRPQ
                     HTETQTKKGRIERWKNMEGKFVLTDKAAIENKHILLVDDVVTTGATLEACGNELLQAE
                     NVRLSIATLCIASR"
     gene            complement(16390..17757)
                     /gene="radA"
                     /locus_tag="PK977_03130"
     CDS             complement(16390..17757)
                     /gene="radA"
                     /locus_tag="PK977_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRF17127.1"
                     /translation="MSKVKTAFFCQNCGYESVKWVGQCPSCGQWNTFVEELVQKENGK
                     KADDWKGYNEEKRNSKTISLNEVSEEKRLPTADTELNRVLGGGIVPGSLVLVAGEPGI
                     GKSTLFLQNGLWLKDVTVLYISGEESEQQIKMRADRIGLKNDNFYLLTETSTQIIFQE
                     IKKLKPQLVIVDSIQTLQTSFIDSSPGSVSQIRECAAEFQRFAKETNTPVFLIGHITK
                     DGSIAGPKILEHMVDTVLQFEGDRHYAYRILRTLKNRFGSTAELGIYEMTDKGMRGVL
                     NPSEILITQKEDQLSGIAIAATIEGMRPLLIEVQALVTQSVYGTPQRTVSGFDLRRLQ
                     LLLAVLEKRGGFHFGMKDVFLNIAGGIKVEDPSIDLAVLCALLSSYEDVPLPNSICFA
                     GEVGLSGEIRAVNRIEQRIAEAEKLGFEKIIVSKYNQKGLSKQKFGIEVVMMGRVEEV
                     YRYLF"
     gene            18252..>18876
                     /locus_tag="PK977_03135"
     CDS             18252..>18876
                     /locus_tag="PK977_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRF17128.1"
                     /translation="MTNEIMLVSLLFLGISFLVSAVLKSKFTKYSKELLSSGLTGREI
                     AEKMLRENGIYDVQVISVDGFLSDHYNPLKRTVNLSPDVYNGTSVSAAAVAAHECGHA
                     VQHATQYGPLMFRSKMVPAVQVSSMLVNWILMAGVIILATTNNPTVLLIGIIVMSLTV
                     LFTLVTLPVEFDASKRALAWLNQTNITNTQEYPKAKDALKWAATTYVV"
BASE COUNT         5004 a         3964 c         3862 g         5828 t
ORIGIN      
        1 gcggtctgat atgcaggaac agcaactgca agccggtgta aaaccttcat tggtaaactg
       61 gtgtcctgcc ccggcagctc cggatggttt attgcatacg gaaatcaatg gcgaagtcgt
      121 aagaaaggaa accacctctt ctcccggtaa ctacatgggt tattacgatg atgtgtacaa
      181 agcattgact acccagatgc ctaaccctgt accagctgcc gatggtatta ataacatgcg
      241 gatcattgag gcagcgttgg agagttttgc ttctggtaaa attgtttcat tctaatttat
      301 gaattatgag ttatgaattt agcattatag gtaagaagag attatttaag tttattaagc
      361 aattcatcat tcttaattca tcattcatca tttttttatc ttcctgttct gtcagcaaac
      421 aaattgctaa atcagccaaa gaagaagtgt tgggcaacaa agccctgctg gctgcccatg
      481 tgggtatcag catttacgac ccggctgata acaaatacct ctacaattac cagggtgata
      541 aatactttgt accggccagc aataccaaaa taccaacctg ttatgcagcg atgaaatatt
      601 tgggagatag tttggtgggg tttatgattc gtgaagtgtc agatacgatt tggttgaaac
      661 cctgtggcga cccaactttc ttgaattcag agtttactaa tcaaagaata ttcaattatc
      721 tgaagggtac atcaaaaaac atttacgttg tttttcatag taaagatgag attcaagaat
      781 atggaagcgg atggtcatgg aatgattatg aagaagctta tatggcagaa cgttcagcta
      841 tgcctgtata tgccaatgtg attagtttcg aaatcaaaga caaccgttta cttgtaatcc
      901 cacctatttt aaaaagacct ttttttaacg agccgatgat acagctaatg gggctgggag
      961 acaagttttc agtaatccga gataaagatg aaaataaatt cagattttgg tctgcaacaa
     1021 cctccttttc gaaagaccga atccctttta aaacaaagaa cgggataaca aattttgatt
     1081 ttttacgaga tacattaaat agatctaaag ataaattagg ctttggcctt tcgcttcaat
     1141 ttgatgagta taaaaaaaat cactcccaac ccaccgactc gctgctcaaa cccatgatgc
     1201 accgcagtga taattttttt gcggagcaaa gtttgttgat ggtgagcaat gaaaagctgg
     1261 gagtgatgaa tgatgcaaag atcattgata ccattctcaa aaccgatttt aaagacctgc
     1321 cgcaaaaacc aagatgggcg gatggcagcg gattaagccg gtataattta tttaccccgc
     1381 aaagttttgt tgctatcctt aacaaaatga aaaatgaatt tggtatggaa cgcatcaaag
     1441 tcatttttcc taccggtgga gaaggaacga tcagcagtta ttacaaagca gacagcgggt
     1501 atatctatgc caagactggt actctttccg gtgtagtggc cttcagcggc tatctctata
     1561 caaaaaagag aaaattgctg attttttcta cgctgataaa taatcatcag tcttcatcta
     1621 ctgatatacg cagggctata gaaaagtttt tgcaggggat aagaaacaag tactaaccac
     1681 ggagttttgt cactccgacg aaggaggagt ctaagagaca acaacaagaa ttcatttgca
     1741 aaatttagat gattcgtttc ttggcttgag gaaaaagcag agattgacta tttcccaaac
     1801 atcctctcca actgctcctg tttaaattca aggtaagcag gatttccatc cggggttgta
     1861 ttcggttgct cacaggcaaa taaatcattt actaactgcc gcatttctct ttctgtcagc
     1921 cgggtgccgg ttttaatggc ctgttgtctt gccagggagc ggactaattt ttctcttttg
     1981 gaaaatttca cttccgggct gaagtgcttg tattgttcca nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nncctgcttc cacatcggca ggtgtaccct gtatcacata gctgtttttg
     2101 ccaaaaggtt caataacata acccaggtat tgcaggtcgc cgataatttc ctccatcact
     2161 gcagcatcag ctggtgtcag ttccagggtc gaagggaaca tgctgcgctg cgtagctacc
     2221 ggtttgtctt tactagctgt ttttaattgt tcatacaata ctctttcatg cgccgcctgc
     2281 tggtggataa ggaggaagcc gttttttgaa ggagcagcaa tgtaagtgtt caggagttgt
     2341 gttaattcgg tatcctggtg aatagtgaat ggcgaatagt gagcaggggc ttgcgacacg
     2401 ggggctgact cctgactccc aattcccggc tcccgactct catagaaatc tctccaattc
     2461 ttcaactcac ttctttcact tttttctata aaatgagcct ggtttttttg cgtaaagcct
     2521 ttgaagatag agcctgatgc agcagcagat cttttttcct cggtgaatgg ttgctgaatg
     2581 gatggcagtt gctgtatcga agcatccaaa tcaaaatcca gtgtaggggt tacactaaat
     2641 tgggccaatg catgtttgat agcggcctgc acaaaggcat aaatgatctt ttcgtcttca
     2701 aactttattt cctgcttggt cgggtgcaca tttacatcca ccacagttgg atccagttca
     2761 ataaagagta catacatcgg gaagctgtct gccgggatca tttcctggta agcactcatt
     2821 acggcatggt tcaggtaagg gctttttata aagcggttgt tcacaaagaa atattgatct
     2881 cctcttgtct tcttcgctgt ttcaggcttt cctacaaatc cataaacatt caggtagtct
     2941 gtttcttctt ttactgttac tagtttagcg ttgtaattat tgcccagcaa ctgaacaatg
     3001 cgttgcttca ggcttccggc ctctaaatgg aaaagctgtt ggttattggc agttagggta
     3061 aacaggattt gaggaaacga catggccact ctcgtaaact cgtcaacgat atggcgcatc
     3121 tcagcggcat tgctcttcag aaaatttctc cttgccggta cattgaagaa taaattcttc
     3181 atagcaatgc tggtaccnnn nnnnnnngct gccactggtt cctgcttctt taccacactg
     3241 ttctctactt cgatataagt acccgtttca tcttcggctc ttttcgtttt cagttccact
     3301 tgtgctactg cggcaatgga ggcaagggcc tctccgcgga atcccatcgt ttttatatga
     3361 aatagatctt caatgtcttt gatcttggaa gtggcgtgtc gttcaaaaca cattctggca
     3421 tctgtctcac tcataccccc gccgttgtca atcacctgaa tcagcgattt accggcatca
     3481 gcaatgatca gttttatttc tgtagcgccg gcatctaccg cattttccag caattccttc
     3541 acggcactgg ccggccgctg aatcacttcg ccggctgcta tctggttggc aatattgtcg
     3601 ggcaggagat ttatcttatc tggcacaaaa tccgctttag ccgccaaaaa taagcatttg
     3661 cagccagccc ctgttgctgt aaaacttcct gatatgaaaa ctactgcact ttggtaacgc
     3721 ttggtttggt tatgggcaca ccatctttat ttaccttgta tttaataaca attaacgggc
     3781 agttatcaat ttttattaat aaagtaaccc agcctgtatt gggattaaat agcatagttg
     3841 tttttccttt cacttttcca cattcccgca aagtagtttc accttcccat tgtgttgaac
     3901 ggatcccttc ggctttcatg agttcttcca aacctttacc agcaaactta ttagcagcat
     3961 tgcctgctgt ttcactatca agcaggccgg gaaatatttc tccccctatt acatcaaaca
     4021 ggtttttagc cagttcatct gtaaaactct cgccgccgta caaagcgctg cctaatgctt
     4081 tggctgctcc tattccaagt tttgctgcaa acgatagacc aggcaatgat tctatggggg
     4141 caaaaataat tccgatcgct aatccgccca atacgtcaga agctgcagta ttagtgttgt
     4201 ttgccaatgc aatagctttt gtggtacaat cacagggtcc tttatcaacc caataatcac
     4261 ggtacaggct tcttaacata cttagggcct gaaattttat ttgtttatcc gatgcatcta
     4321 ttggctcggg taatnnnnnn nnnnnnnnnn nnnccggctt tggtgcactg ttctttgcct
     4381 gctcttcttc ctctttacgt ttttttgccc tttcctcttc taactgcttt tctctttctg
     4441 cctggagttt atcagcagcg gcctgtgctt ttttcatttc ttcttcggca gctttcattt
     4501 cttcttcggc agctttaatg tcattgccca caccttgtgc ttcgcttgct ttttcatcag
     4561 cagttttttc caggtcagcc tctttttgct ttttcttgtt tatgatgtca tcaagctgtg
     4621 aaaggaaagc ctccagatct gctactgtgc ggggtttacc gggtaattca ggattaaaat
     4681 ggcaaacacc aggatgagct gcacaccatt ttttcaatgc atccagcaaa gcttgtattt
     4741 gcctgcacag ttcatctatt tgtgtttctg cggcggccat ggcatttcct tttttggcag
     4801 catcttcagc cttgtctttt tcctttttca gtttatcgca ttcttcctgt gcctttgtaa
     4861 tttctgcttc caaagccttt aacctgtttt tagccgcatt ctgtggtttt cttaaagctt
     4921 tcagtttact ggaaatagcg tttgattcac tccaaatatt tgtgttggaa ttttcatcac
     4981 ctacatagcc ataccaaaaa cttccgtctt tatgatagcc atgtcctcct gaccagttat
     5041 ttcccaggtg aagcgcatcc agttgtttta aaagattttc tatttcatta ttgagattat
     5101 tcagttcttg ttgtgcattt tctttttcct gttttagttt attgagcctg tcttcacaat
     5161 cctttgcccg ttgtgaagct tcatctgctg tcttttgcaa atcagccggg tcgggatttg
     5221 gaggcagcgc tgctctcagc tttgccaatg aatcagtcaa ttttttcaat gaatcggcgt
     5281 atagcccggg tatcttttct atggttttgt ctattgctgc cagttcatca gccattgttt
     5341 tatttctgtc catttcttca atcagctctt ccagctttct tcttagtcga gctaattttt
     5401 cctgaattct ccaaagattt tccacttgtt gctgcacatt ctgattggca ttactcagtt
     5461 gtgcatctgt tacagtaaag tttactcttt cacccggcat agttttaata gtggccgggg
     5521 ttaattgcag atatacaccg gtgtatagtt ctgtaatcgt actgtttttt cctgctaccc
     5581 ttgcccgtat gatatgctgg ccatcattcc cgaggggcgg taaccagttt gccgaccaac
     5641 tggcaccatt aaaagaggct ctccctgcct gttgccattc tgtgcctgaa accggttttt
     5701 cagaaaagat tacggctgca acatcatcat tcaatacatt tacctgtaga gtaacctggt
     5761 aagtttgagc atttatattt cccggtgaga gtaatatagc aggtggaggg tttgaaattt
     5821 ttggattttg attatccggt gatgtattat agaatacagg agggttggtg ctaccgccaa
     5881 gggcaaggca tgtttgcaat ggtatcaggc aagattgctc attaatataa cagttgcaga
     5941 gtgatgctgc attgcctgtg gctgaagccg gttctggtaa atctaagttc acatttacag
     6001 aaaagttccc tgttttcaca ctcttcaaat caaatcgttt tgtaaagctt cctgccacag
     6061 aaacagttgc cggttgaatg tgtattacct gtgcatcgcc acctgtcatc atcactgttg
     6121 ccgtactggt atttaccacg gatagtgtgg cagcagaagg aaggttttgt aaacctgtaa
     6181 taattacatc tatataagta ctttctcctt tttgcaaatt gaggcgtcct gcgtttacat
     6241 ccagatttac agcacatatt ttttgctcag ttgtcttgtt attttcactt atcaatatgt
     6301 tatgagcgcc gctggttacc tgaggcagct gaacaatgca ttgccttggt gattcggcca
     6361 ggatttcaat gtgtctgccg tctatcatac attttgtatt ttctgcgttg ccgtcaaact
     6421 ggcctttcat tggtaatggg cttgccgtta atgcatgagt gggaatagat agatttgttc
     6481 tggttgcaat attgcttttt aatggaatca taagtgatga aatgatttta ccattggcag
     6541 acaaagtaaa atttgtattg ggccccttta gcgtataatt gaaaagtccg gataatccct
     6601 gtttaccgaa actaccatca gcatgaccaa gctggtattt ttgaagtgat tggatgttgc
     6661 ggcttttttc tttctccgta ttgccggcgg cttccattac cacggtaccg gagatatgat
     6721 ccccctgtct aatatcttct ggcagataaa tttttatttt tccttctgac aatatgaatt
     6781 cagcagttgt caatcccttt tctgagttaa ttatttgcgc ctttgctgcc aattgactga
     6841 ttaataacaa acatgtaaaa atctttagat gaatggtgaa aggtttcata actgtttagt
     6901 ttaaaagatt aataaactgg tacagtagcc tttcaggggg gcagagtaga attaaaaggt
     6961 aagattttaa tacaatataa acaggggaaa gaactaattt taagcgccaa tgaaaaaatt
     7021 agttatgcaa agattacaac gacttgtctt tctgttagtt ttcctgcctt tattttccaa
     7081 cggccagtat aaaagcaaac tgggaaagca gcagtgggta gacagtgtgt acagcagcct
     7141 gagtgatgat gaaaagattg cccaattgat ggtggtccgt gcccattcta acctgggacc
     7201 agatcatgtg gccaaagtgg taagagatat tcagaaatac aatgtgggtg cactttgctt
     7261 tttccaggga ggcccggtaa ggcaggccaa cctcaccaac tattatcaaa gcattgccaa
     7321 aacacctttg atggtaacga ttgatggaga atggggcctg ggtatgcgcc tggacagtgt
     7381 aacaaaattt ccttaccagc tcactctcgg tgcgctggat gatgcagaac tggtgtacaa
     7441 aatgggtgta gctgtcgggc agcaatgtaa aagaattggt gtgcatgtca actatgcacc
     7501 tgtagtggat gtaaataaca atcccaataa ccctgttata ggctatcgtt cttttggtga
     7561 agacaaaaac agggtttctg cattgggagt ggcttatatg gggggcatgc aggatgcggg
     7621 catcatggcc tgtgccaagc attttcccgg tcatggtgat gtggatgtgg attcacatta
     7681 tgatctgccg gtgataaaca agaccaggga acaattaaat gaaatggagc tggcaccttt
     7741 tcaaagtatt tttaaagccg gtgttaagag tgtaatgatt gctcacctgt acattccttc
     7801 aatagataat acagcgaata aagcaacatc cctatcgaaa aataatgtga ctgatttgtt
     7861 gcgcaaagag atgggttatg aagggctcac tttcactgat gcgctggaga tgaaaggtgt
     7921 aagtaagttc ttccccggcg gcaccatcgt agtggaagca ttggtagccg gcaacgatat
     7981 gctttgccta ccggaaaatg taggggatgc cattgctgca gtgaaaaaag cgatagcgga
     8041 aggcaaattg tcttggaatg atatctatgt aaaaacaaga aaagtgctga atgagaaata
     8101 tgacctcggt ttgtttgagg tctggcctat caataccaat aacctgttaa atgatcttaa
     8161 tgccaaaacg gatgctattc gaaaagaagt agcggagaaa acaatcacat tattatcaca
     8221 agctgctttt aaagcttccc gcactgatta tgctggtata ccactttcaa aaaaagaaaa
     8281 gatcgcttat gtgggaattg gcagcactga attgaacact ttcggcaagc gaatgaaaga
     8341 tgattttggg gcagatgttt ttcttttctc ttataaagat ggaaaagaaa aggcacagga
     8401 aatattgaag caggtgccta aggatggtaa atatgatgca attgtaatag gagtacatga
     8461 ttactccctg cggccaacaa ataatttcgg aatttcacag gcagctatcg atttatataa
     8521 agaactgaac ttcatcaaaa ctgttacact tacgtttgga aatgtgcttg ccaccaaaaa
     8581 tttctgcgat gcatggacac tagcggcctg ttaccaggat gaccctgtta cacaaaatgc
     8641 agcagcagac ctgttgctgg gtaagttcaa ggcaagagga aaactgccgg ttaccgtttg
     8701 cagtttcaaa ccgggcgatg gaatagtagc ggccaaaact gctgtggaag aaatgcaggc
     8761 taaattgaaa gtaattgatt ccatagccca ggatggtatt agtaaaaaag catttcccgg
     8821 ttgcgtagtg cttgctgcgc ataagggaga aattatttac cataaggcat ttggcaacta
     8881 tgagtacgaa ccttcctcac caaaaatggc agctgaaagt atttttgacc ttgcgtcagt
     8941 aaccaaggtt tctgccacca ccgtatctat aatgaagctg tatgaggaag gaaagatcaa
     9001 gttggacgga aagctgaagg actatcttcc ctatacaaaa aacactgata aagaggattt
     9061 gaagattgat gaaatcttat tacaccaggc cggattggtt ccctttattg ccttttataa
     9121 agaaacaata gatagcagcg gggcgcccaa accggctgta tatgctgcca agccgcaaaa
     9181 aggatatggc gtaagggtag ctgaaaatgt ttacctccgc aatgactggc aggatacgat
     9241 gatgatgcga atacttaaaa gtccgctggg accgaagaac aaatatgtgt acagcgacaa
     9301 tgattttatt ttcctgggta aaatagtaga ggaagtaacc gggttgacac tggatcaata
     9361 cgtacaaaaa accttttaca acaaaattgg tatgtattcc acaggcttca aacccaggaa
     9421 tcgtttccag ttggaatgtt tagtgccgac ggaaacagaa aagcattttc gtcagcaaac
     9481 taccaggggt gatgtacatg atgaaggggc ttctatgttt ggcggagtgg cagggcatgc
     9541 cggtttattt tccaacgcat acgacctgtc gttactttac cagatgttgt taaatggggg
     9601 cgagctaaat ggcgagagat acctgaagcc cgaaacaata aagttgttta catcctacaa
     9661 cagtaatatc agccgcaggg gctttggatt tgataaaccg gaaaaagaca atgcaacccg
     9721 caaagaaccg tatccttcag cattggcttc gcccgaaaca ttcggacata ccggttttac
     9781 cggaacctgt gtgtgggtag atcctaagta tgacctggtt tatgttttcc tttccaaccg
     9841 ggtttatcca acaagggata atggaaagct atcccaattg ggcatccggg gtaagattca
     9901 ggatgcattg attaaggcag tatctggtca ataaataatc ttaatttaaa ccaaccataa
     9961 tgaaaccttt ccttactgtt attttactgt ttacatctct gctattctat gggcagcaca
    10021 ctccgaatga atttaaaaag tttagaatcg cttctgctgc tgaaacctgt tcttttaaag
    10081 aggatggtgt tccgcaaaag ttcacatata cagcttacta tgacagaaat gggaatgaca
    10141 cagcttatta tcatgacgag ctttgtatat atcgaaaaat ttatacgtta gatgaaaaca
    10201 acagaatcgc aatgttgatt atgtatgata aggatggcaa agagagggaa aaaaccgaat
    10261 atacctatga agctaatgga gactattcaa caaaaagcta tcataaatta ttcgatgcgg
    10321 tcacgataaa atggtttgat aaaaccgaca ggttgttaaa agagcagcgg gatgaagaaa
    10381 caacacttat ttatgattac aattcaaagg ggcagcttat acaagtgaag acgaagcccg
    10441 ggcccaatag caaaggagag attgagcacc tgaaatatac ctataacaac aaggggcaat
    10501 gtattaaaga acaaagcctt ggtacctatc cctgggttaa gacattattt tatgacgcaa
    10561 aaggaatgtt gacaaaaaca atcacaaccg gaaaagaagc cggtgtggcc tataccaata
    10621 ccaatactta tcaatatact ttctggaaat aattaaacct gtttgtcttt tccctggttg
    10681 gggttatcag gccgctgttg gttacggtta ggattgccag gtctttgctg gttcctgttc
    10741 ggattgcgtt gctgtccctt ttgctgtcct ccctgttgtc ttctttgttg ctggccaccg
    10801 ccttgttgtt ggccgccctg ctgacgttgt tgtggccgct gacctgactg ccggtcaggt
    10861 ggaagttctc cacgcccacg ccgaacagcg cgccgccgta gacatcctgg cagtactggc
    10921 cgctgacctg actgccggtt aggttggttt ctttgttgtt gctgttgttt tttctttttc
    10981 tctgcctttt ccagtgttcg aagactaatt tgccctacca gttccactgt ttccggttct
    11041 gcttcttttg gcttaccggt cgtaacttct acaggttcca gttcatcagg agttatacct
    11101 tgctggttca gcgattttat ttttcgaacc ctttcaatgg ttagcgggta ctgcttattg
    11161 ctgtctggca atgtgtacca catcaggttc ttgaaaatat ctttcttgat aaggaatgca
    11221 ttgcccttgg ctacctgtat cacatcgcaa ttgtcaggaa aatgctgtaa ggcatctaaa
    11281 taagtatcca gttcgtagtt caaacaacat ttcaaacgtc cgcattgacc gctgagcttt
    11341 gtctggttga tggataagtt ctggtaacgg gctgctgctg tatttactga cttaaagtca
    11401 gtgagccagg tgctgcagca aagttcacgg ccgcaactgc cgatgccgcc caccttacct
    11461 gcttcctgtc ttgcaccgat ctgacgcatt tccactttta ccttaaattc agaagcatac
    11521 actttgatca gctccctgaa atcaacccgt ccatcggcaa tataaaagaa agtggcttta
    11581 cgtccatcag cctgcatttc tacctggctg atcttcatat ccagcttcag ttgcttggca
    11641 atggcccggc tacgtataac tgcttctggt tcccgggctt tgttctgctt ccatatttca
    11701 atatcacggt cggttgcccg gcgcaaaact tttttcattt cggggttatc ttctttcact
    11761 cccctttttt tcatctgcag gcgaacgatt tcaccagtca gcgacacttc tcctatatca
    11821 aaaccactta ccccttccac ggcaatcagg tcgccttttt caaaaggctg caacatagga
    11881 ttgcggaaaa aatctttgcg ggtaccctga ctaaaactca cttcaatcac tttacaggca
    11941 ctgtcaaaat cagccatggg gaggttgtgc agccagtcgt aggcgttcat acggttacac
    12001 ccgccggaac tgcatccgcc gttactctta catccattgg gttttccacc cgtagccgtg
    12061 ctgcagccag tacaactcat tgctgatgtt agatttcagt cccgatagtt atcaggaaat
    12121 gattgaataa tttgaggaaa gaatgatgaa accagctgta ataattctac caggctgctg
    12181 ttgcgtcgca ctcttgtacg ttctgtccgc ggcccatcaa agctgaaacc cgttgagcag
    12241 cagttgtata tgtagaagta gtagtgaaca caaataatag tttcatcaaa tcttatgggc
    12301 ccttttcttt tcctcctggt tctcttttag cctcatagcc catcctcaat ccatcaaaaa
    12361 tacaatctta tcctgaatta tgtggtatag tttaatagtc agggcatgga agagcatttt
    12421 gccatttgca ttgcgttcta tgtagtagct ggccttatca atctcttcaa taatagcctg
    12481 ttgttgttcg attccggcaa tcttattgag tctttctgca aaatcccttt ctgtgccaaa
    12541 ggcccctggt gtatttccgg tagctttaag cagaatggcc tgttccagca agtgattgaa
    12601 atagcgtaaa aattgttttt gtttctcccg ccccagccgg ctgatctcct ccacccattt
    12661 tgcctgggcc accggtcctg tcttcagcat ggcattcagc cattcccgta aaaggctttg
    12721 ccagtcctct tcggcatgtt gcaccagttg caaagcttcc cggtaattac cttcgctaac
    12781 actggcaacc tggcgggcaa tggatgcttc tgttttattc cggctgatca aagcctcttc
    12841 aatttcttta ttttccagtg ccggaatctt tatcaactgg catcggctta caatggtggg
    12901 taatatgagt tcttcatttt ccgccaccag aatgaataaa gtattggcag gcggctcttc
    12961 aattattttc agcagcttat ttccttcctt ttccagttct tccggcatcc aaagtatcag
    13021 tactttgtat tcactttcaa aactcttgag gctgagtttt ttgatgatct catcacattc
    13081 cctggaagtt attttgccct ggctgttctc tttttccttt atcagttcaa tccagtcaaa
    13141 caaatttcca taagggttta atttgatgaa ctccctccac tcggtaataa aatctgtagc
    13201 gattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnttgagctt tttgacaggc cggacaggca ccgcaggaat
    13321 cggtcaccgg ttgattgtta acggtaactg gttccgggtc tccgaacaat gagggacctg
    13381 cctgtatagc agtatgcttt ccctgtactt tttcacaggt tacatattgg gcaaaagcca
    13441 atgctaatgg cagggctccg cttccttcct tgcctaaaaa caatagtgca tgactcaacc
    13501 ggttatgatg taccagttcg gccagttgtt gttttacatc tttttgtcct atgatatcgg
    13561 agaatgtcat gtaaggcgaa agtaaacctt ctggcaataa aaaagcaata ctgaaaattc
    13621 agtattgctt caaatttttg aggaatatga attagttcag gtattttaaa atttcttcac
    13681 tcatcggggt tgttcttgga ctaaaggtgg cgatcagttc tcctttttca ttcaccagga
    13741 actttccaaa attccaggtt actggatctg taaagccttt ttcctttgcc tgggacttca
    13801 accaggtgta taatgcatgg gtgtcatcac ctttaactga tatcttttca gccatgggaa
    13861 aagttacgcc atagttctgc ttacagaaat cttttatctc actattgttc aggggctcct
    13921 ggccggcaaa attgttggca gggaaaccaa cgatcaccag cttgtcttta tactttttgt
    13981 acaattcttc cagcccttca tactgaggcg ttaacccgca tttggaagca gtattaacga
    14041 tcaatatctt ttttcctttg aatttggaaa agtcgatttc ttttccgtcc agcccggcta
    14101 ccttaaaatc gtaaataccg ccagtcagtg tggccgataa tattaatgtc aataaaagcg
    14161 atttcatttt cttttgttta tgtcaataac aaaggtaaac ttgttctgtt cagagttggg
    14221 taaattaata ttacctggtt tattgctttt cgaaacatcc cagatcaggc aaacccacag
    14281 ccctgggatt accatcgagg tcggtcgtta cggatgtgct ggttcccttg ttaacagcag
    14341 gagatgttga ttttaaccgg aaatcataga aatttcttga agtattgatg ctgtcaaact
    14401 gcggtgtttg attattgatt atttggttgg cggatacacc ggcaggtgca gcctgtacct
    14461 tccacaaatt gtaatcaaaa ttgacattga aaacagtatt tccgcttttt aataccacta
    14521 cttcatcatc tactaaaccg ttttgtcccc aaaaaataca gtttcgaaaa attgcattta
    14581 aatcagccgt actattattg gtaaagtttg taagagctag tacgggatct ttatgatcaa
    14641 cataacggtt ggctattgtc actattgtgc aatgggtaaa ctggtaatcg ccgcctttta
    14701 ccaggaataa atttttgcca caattgctga tcaggcaatt tctggccctt atactgctgt
    14761 tgactgctac gatacctgca tcatagttat tgtcgatcac acattcattt aaaattagtt
    14821 ttggactggc attgggagag gggtccgtta tacctattgc ctggaaagca tttctgataa
    14881 cagcataatt cagcacatta tcctttgaac cagcctggaa aaaaataccc ggccagtttg
    14941 caggaaaatc tttgtagggt tcatctaatc tgtcgcctgt aaaatacacc ctgtccgctg
    15001 tatcttttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnngtt aatggtaagt gtttgattgg tattgatggt
    15121 taaagaacca aggatcacat atggaaggtt gttattccat atctcattag tggatacaac
    15181 cttattccga agaaaattgg cattttgtcc ccatgcctct aattgcacca ggcggtcttt
    15241 gccattataa cttacctgaa tactgtcccg tattacaaaa ggcagattgg cagcacctgg
    15301 atttacattt acttgtacaa atacataaag gctgtcgttt gcatttattt ctaaattatt
    15361 aaaacgggtg ccggctgtgc cgtcaacatt cattttaaaa gctgaggatg ttcctcctga
    15421 aagttgtaaa gaagaaatca gcagtttctg gttattctcg ttaatgattt tgaacgttct
    15481 gtaagtggat ccggcggtta caaatacggt atcatacttt aatgtatctg cactgatcgt
    15541 tacccgggca tcaggtgaag tgatgaatga atctttccgg caggaaacaa aaaggaggag
    15601 aaaggatatg gttaatagcc caaagatttt cattattgta aaaatataac taagctgcaa
    15661 aaagaggaaa tgtgatttaa gccagtcgtt ttagcgggat gcaatacaaa gggtggcaat
    15721 actcaatctt acattttcag cctgcagcaa ttcgtttcca caagcttcca gggtagcccc
    15781 cgtagttact acgtcatcta ccaagagtat atgtttattt tcaatagcgg ccttgtcagt
    15841 caaaacaaat tttccttcca tatttttcca ccgttcgatc cggccctttt tagtttgtgt
    15901 ttcggtgtgt tgcggtcttg tgatcacagt atttaataca ggcacatgca tagtttctgc
    15961 cattccatgg cataatacag ttgcctggtt ataacctctc cttctttctt tagctggaaa
    16021 gagaggaagc ggtatcaatg catctacagg gaatctgcct gagtttttta gttgctcacc
    16081 catgatacgg cccaattgaa ttcccagttc tttgttgcct ttatatttga gctgatgcat
    16141 gaggtgctgc atcagtgatt ctttagtaaa ataaaattgt gcagatgctg ctgctactgg
    16201 caaccgcccc caaaaagttt tttcagccgg gttgccggca tgcagttcaa actgggtttc
    16261 cggcatagct tccacacaac gcatacataa agcagagtct ttgctgagca aatcactgcc
    16321 gcaacctgta cagacatggg ggaacaacag gtgaagtact gattctttta tttctcttaa
    16381 taatgtcatt taaaaaagat aacggtagac ttcttctact cttcccatca ttactacttc
    16441 aatgccgaat ttctgtttgg aaagtccttt ttgattatac tttgaaacga tgattttttc
    16501 aaatcccagt ttctctgctt ctgcaatacg ttgttcaatt ctgttgaccg cccttatttc
    16561 accactcagg cctacctcac cggcaaaaca gatgctattg ggaaggggga catcttcgta
    16621 agaggacaac aaagcacaaa gcacagccag gtctattgat gggtcttcta ctttgatacc
    16681 tccggcaata tttaagaata catccttcat tccgaaatgg aatccgcctc ttttttcaag
    16741 aacagctaat aataattgaa gccggcgaag atcaaagccg cttaccgtcc tttgcggagt
    16801 accgtaaact gattgtgtta ccagggcctg cacttcgatc agtaatggcc gcattccctc
    16861 aatagtggca gcgatagcta taccactcaa ctggtcttct ttttgggtaa taagaatttc
    16921 actggggttg agcacacctc tcattccttt atcggtcatt tcataaatgc ccagttcggc
    16981 tgtgctgcca aaacggttct ttaatgtccg tagtatacgg taggcataat gtcggtctcc
    17041 ttcaaactgc agcacggtgt ctaccatatg ctccaatatt ttcggaccgg ctatacttcc
    17101 atcttttgtg atgtgtccaa tcaggaatac cggagtattg gtttctttgg caaaacgctg
    17161 aaattctgca gcacactccc tgatctggga tacactgccc ggtgaagaat caataaacga
    17221 ggtttgtaat gtttgaatgg aatcaacaat taccagctgc ggtttcagtt tttttatttc
    17281 ctggaaaatg atttgtgtag aagtttctgt cagcaagtag aagttgtcat tctttaaacc
    17341 aattctgtca gccctcattt ttatttgctg ctcactttct tctccgctaa tataaagtac
    17401 cgttacatcc tttaaccata aaccgttttg taaaaacaaa gttgattttc caatacccgg
    17461 ttctccggca accagtacta aactgccggg tacaatacca ccgcccagca cacggttcaa
    17521 ctctgtgtca gcagttggca atcttttttc ttcgctgacc tcatttaatg atatggtttt
    17581 gctgtttctt ttttcttcgt tgtacccttt ccagtcgtca gctttctttc cgttttcttt
    17641 ttgcactaat tcttccacga acgtattcca ctgtccacat gacggacact gacctaccca
    17701 cttcacactt tcgtatccgc agttttgaca aaaaaaagct gtctttactt tactcatgca
    17761 ataaaattaa ctgatgcagg gggttcttta aaaagtttaa acattgatat gctggaatga
    17821 agtattgagt gaccaattca tttcttcttt aatgaaaaaa gtcatccttt tttttgatga
    17881 cttttttgct tctttttaaa atgtaaaaag ggccaatctt gcgactgacc ctcttactta
    17941 ctaacccatt aaattctatt gctaaattac aattatgccc cacatttcaa aataaatttt
    18001 tgctgctgtg aataacttcc ctgtcctgtc aatttaaaaa agaggctaat aagaaaaatt
    18061 tattcaattg cataaagtat taaatatcat tcatttatgc aatatttaat gttcgacagg
    18121 tcttaagtaa atttccagaa cttattgttt gttatcactt tgaaatgaca aaaaggctga
    18181 aaaaaatgac ttaatccaaa tggtaatatt tttgttatca atagtatcaa tcaaatcaat
    18241 attttataga catgacaaac gaaattatgc tggtttccct gctctttttg ggcattagtt
    18301 tcctggtttc tgcagtgctt aaaagtaagt ttaccaagta cagtaaggag ttgctttcca
    18361 gcggtcttac aggccgtgag atagccgaaa aaatgctccg ggaaaatggc atttatgatg
    18421 tgcaagtgat ctctgtggat ggattcctct ccgaccatta taacccttta aaaaggacgg
    18481 ttaacctgag cccggatgtg tacaacggca ccagtgtgtc ggcagctgct gtggcagccc
    18541 atgagtgcgg ccatgccgta cagcatgcta cacaatacgg gcccctgatg tttcgcagta
    18601 agatggtacc cgccgtacag gtaagttcaa tgctggtcaa ttggatactg atggcaggtg
    18661 tgattatttt agccactaca aataatccaa cggttttact tataggaatt attgtaatga
    18721 gcttaacggt gttatttaca ctggttaccc tgcccgttga gtttgatgcc agcaaaaggg
    18781 cattggcctg gctgaaccaa accaatatta ctaacaccca ggaataccca aaagctaagg
    18841 atgccctgaa atgggctgcc actacttatg tagtag
//
LOCUS       DAORLS010000021        18804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1577_length_18804_cov_2.084004, whole genome shotgun sequence.
ACCESSION   DAORLS010000021 DAORLS010000000
VERSION     DAORLS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_18804_cov_2.084004"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>264)
                     /locus_tag="PK977_03140"
     CDS             complement(<1..>264)
                     /locus_tag="PK977_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17129.1"
                     /translation="PGKMPLLSRADFNCPGGGKMMLDGKASRIDDETVIKGEVHLDKT
                     GQPVFEDEWDFVIDNYTVGANDPDIIDSIALIAKHPTPVIINTE"
     assembly_gap    265..274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<275..571)
                     /locus_tag="PK977_03145"
     CDS             complement(<275..571)
                     /locus_tag="PK977_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17130.1"
                     /translation="MKPEKRIFVAFGLLGLLLSADSYSQMITGTWKGRINRQKVEVKI
                     IQKGDSLTGTSYYYESPGNYRRYSIKGYFDARTNEAVWWDDQLLEEKSGRFSIGT"
     gene            783..>1863
                     /locus_tag="PK977_03150"
     CDS             783..>1863
                     /locus_tag="PK977_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17131.1"
                     /translation="MRFLYPQTENVHQLSISPVLRKISIRFIFSFALMAFSASLFAQN
                     PGKANYKLINENDGSVRYEISNGGKVVFTEKIGNISDNPQAISAYADKKAKAALRAYQ
                     QIGNYPQQEIADRIKKQMANDPEIAQRIVPEGACTYNSTIDGTDASMADRLLRPAGTP
                     ASCAVPVACPGPFGGVGPYFYETYTLTNSTGTNQCVTVNINWVSGTGTAVHVTAYSGS
                     FNPASLCTNFLADPLTSAVSGTPQSFSFDVANGATVVLVATSPNGGDVTYSMTVNGLY
                     ADPVIVSQPLAQSFCGSGTPTFSVGATGVGLGYQWERSTDGGATYLPIAGANSSSYSP
                     GPVTIAANGYLYRCVVTTSSPGCSGS"
     assembly_gap    1864..1950
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1951..>2911
                     /locus_tag="PK977_03155"
     CDS             <1951..>2911
                     /locus_tag="PK977_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17132.1"
                     /translation="NTTGSTTITGTASGGTVGGTGTFSSGTINLAIVDNGSVNSTIAL
                     PAINITNANDLKVRINANHTWVGDLTFQLTSPCGVTYLFDRPGLNPPGTCCGSSSDLA
                     GVYEFDLAAATAFPEAPASPVPTGIYRPTDAFGAPHNWAGAAFPCSAGGNWTLSVADG
                     AVGDVGTLVDWAIIVPGGYTHTLTGPGTITQNPSSGPNNATGSFTVSGLAPGTYNYTF
                     TSTDAVGCSVSTNVSVQVNPIPSLTISPSSAAICPGGIVQLTATGTGGSLTTFSSAAA
                     ITIPASGVATPYPSTINVSGLPTGGVNVATVVLNNFSHTFPDDV"
     assembly_gap    2912..2972
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2973..6391
                     /locus_tag="PK977_03160"
     CDS             <2973..6391
                     /locus_tag="PK977_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF17133.1"
                     /translation="DVGGGTDAVNLTYTFDDAAASLMADAALNPTGTYRPTNIGAGDN
                     YPAPGPLTAPTATTLATFTGNPNGNWSLYVVDDLVGDAGSISGGWSITFNAPPPANAV
                     FTPQNGLFNNAGATIPYTGTAQSTVWASPVATTTYTATTTVNGCTSAPVNVTITVNPV
                     TTITTQPANSAIVCAGSAATFSVVAAGTAPLTYQWQISTTGAGGPWTNLTNTAPYSGV
                     TTATLTINPVTAPMNGNLYRCVVTGGCGTATSNAGTLTVDQLSHTGVAANPAVTCSPG
                     STTIAGTAVNGSFAVSLGTLGSSGTINLPIPDGNPTGVNSTIVLPANSIANANNLRVR
                     INTTHTWVGDVRFTLTSPCGTTFLFDRPGVPASTFGNSADLSGVYTFDVTAATIIPET
                     GGGVIAAGSYRPSDAPGNPHNWAGLTFPCATAGNWVLNASDAAAGDAGTLVSWEIIGP
                     VPANYTHTLTGPGTIGAPVLSGANNATASFPVSNIPAGNHTYVFTSTDGNGCSVSTNV
                     NVLVNPTPVVTIAPAAPVICAGNIQQLTATAVPGVTQTFNSATLNLAIPDNNPAGVTT
                     PAIVLPAGISITNAADLKVRINANHTWVGDLIFRVTSPCGTTFLFDRPGVPATTFGNS
                     QNLGGVYTFDLSAATILPETNSGPTTIPAGSYLPSNTAGAAHTWAGATFPCAATGNWT
                     LTMSDNGGGDTGSLIDWGIIVTTPSPVVFSPTTNLYTNAAATIPYTGTPVTTVWAAPP
                     TTTTYTATATVAGCTSAPATVTVTVNQLPAITVQPQALSAPVCPGFNVNYSVTATGTG
                     LTYQWQISTDNGTTWTNLVNNGTQYAGVTTSTLTLLNVQTTQNGHRFRVIVSGVCPPP
                     ATSSSVTLVVATPPTITTQPANLTVCAGVNATFTVAATGVPAPNIYQWQVSTNGGVTW
                     TNLTTGGSYTPTLTVSPTTTALNGNRYRVIVTNSCGQNVTSNAAILTVNPLPVVSATD
                     LWNRRICISDTLVPLVGTPTGGSWSGIGVSGFNFVPTATAIGTYTLTYTFTNSFGCTA
                     TDTTKAIVVDCPERIRLLRDNAVILFPNPNGGQFNIRINSVLYNYLGMNVYNSAGQLI
                     SQKTFSGLAYGRVVPIDLTHLPSGVYMVKFFYDDGIRTSEKTFPVIIGRQ"
     gene            6471..7178
                     /locus_tag="PK977_03165"
     CDS             6471..7178
                     /locus_tag="PK977_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF17134.1"
                     /translation="MNQALAFKNISKTSGISFNRVIPFDPVKDKLVLLDFTAANEELE
                     PGILNDITRFSQWVNNKLEHSHARYGIGGYAEHRTVYSASKVFDGNNDGEEPRRLHLG
                     TDIWGKPNTAVIAPLDGIVHSFAFNNRFGDYGATIILSHNLQGFTFFTLYGHLSLNSI
                     KNIREGQRISAGEIFAEFGIPAENGQWPPHLHFQIILDIGNWHGDYPGVCKFSEKEKW
                     LANSPDPDIILQLNQYL"
     gene            complement(7185..7832)
                     /locus_tag="PK977_03170"
     CDS             complement(7185..7832)
                     /locus_tag="PK977_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF17135.1"
                     /translation="MKVGIIGSGQVGRVLATGFLKEGHQVMLGTRNISKEEVVKWQQE
                     NRSGLLGSFQETAQFGEVIVLAVSGLVVEDAISLAGKEHFFEKIVIDTTNPLAAVPPD
                     NGVLKFFTTLEDSLMERIQNMIPDAKVVKAFSCVGNAFMYKPSFKEGKPTMFICGNDA
                     EAKEKVSVILTSFGWETEDMGRVEAARAIEPLCILWCIPGFIRNQWSHAFKLLKA"
     gene            7881..8909
                     /gene="ruvB"
                     /locus_tag="PK977_03175"
     CDS             7881..8909
                     /gene="ruvB"
                     /locus_tag="PK977_03175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRF17136.1"
                     /translation="MANPNLNSDKESLSSADVAFENSIRPSQINDFSGQDQIIENLRI
                     FIKAAKIRGEALDHVLFHGPPGLGKTTLSRIVANELGVNIKETSGPVIEKPGDLAGLL
                     TSLEANDVLFIDEIHRLSTVVEEYLYAAMEDYRIDIMIDSGPNARSIQINLNPFTLIG
                     ATTRSGLLTAPLLSRFAIKSRLEYYKSGVLNKIIQRSAGILGTKISKDAIEEIGRRSR
                     GTPRIANGLLRRVRDFAQVLNDGIIDLGITQHALKALNVDEHGLDDMDNRILSTIIDK
                     FKGGPVGITTIATAVGEEAGTLEEVYEPFLIQEGFMQRTPRGREVTAKAYEHLGKTKP
                     GSAGTLFS"
     gene            complement(8964..9659)
                     /locus_tag="PK977_03180"
     CDS             complement(8964..9659)
                     /locus_tag="PK977_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17137.1"
                     /translation="MEAKKPKILLCEDDPNLGSVLKNYLELNDYDVTLERDGRLGLAA
                     FQREKFELCLLDVMMPNMDGFTLAEEIRDVDPDIPLFFLSAKTMKEDVIQGYKLGADD
                     YITKPFDSEVLLLKIKAILKRNEEQNKESENKEYDLASYHFNPKLRQLIHDGVTQTLS
                     PKENELLKMLAEHLNDLLPREAALKKIWGSDTYFNGRSMDVYIAKLRKYLKDDPKIEI
                     VNIHGNGFRLVVQ"
     gene            9800..10171
                     /locus_tag="PK977_03185"
     CDS             9800..10171
                     /locus_tag="PK977_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HRF17138.1"
                     /translation="MHITPDNRLKKLIVIGDRLLIKPTRPDERTASGLYLPPGVQEKE
                     KVQQGYVIKAGPGYVIPMPVEEESWKGEEEQVKYVPLQAREGDLAIFLLSGATEVMYE
                     NEKYFIVPQSAILMLEREEEL"
     gene            10205..11752
                     /locus_tag="PK977_03190"
     CDS             10205..11752
                     /locus_tag="PK977_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRF17139.1"
                     /translation="MADSTKFLEIVKAGYTFKGESFKIGCAMLNGEVVPGTDVLLPFK
                     TLNRHGLIAGATGTGKTKTLQVLAEGLSDASIPVLLMDIKGDLSGIAAAGTVNDKITD
                     RCQKLNIEFKPTAYPVDLLTLSEQKGARLRATVSEFGPVLLSKILGLNDTQGGFVAMI
                     FKYCDDNKLPLLDLKDFIKVLQYVSDEGKAELEKTYGKISTTSTGTILRKVIELQQQG
                     ADLFFGEKSFEVEDLMRISDDGRGMISIVRVTDLQDRPKLFSTFMLQLLAELYATCPE
                     EGDMDKPKLVMFIDEAHLIFQEANDALLQQIETIIKLIRSKGIGIYFCTQNPMDVPAD
                     VLAQLGLKVQHALRAFTAADRKVIKQTAENYPETDFYKTEDLITQLGTGEALVTILNE
                     KGIPTPLVHCMIRPPQSRMDILSDMEIDALVSKSKIASKYNQVIDNVSAYEILTEKLE
                     EASKKEEEQKQTEQKEEKKTTTKKEKSVFDDPTIRSMTRTAGNTIIRSLLGVLGLGGR
                     SRRKSLF"
     gene            complement(11744..>12834)
                     /locus_tag="PK977_03195"
     CDS             complement(11744..>12834)
                     /locus_tag="PK977_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HRF17140.1"
                     /translation="IGNKTRVIVCTTAFGMGIDKPDVRTVIHADVPDCLENYYQEAGR
                     AGRDGKTSYAVLLYDNRDLHELEEMADLRFPGLDDIRNVYQSVANYLQIPSGSGESQY
                     YDFDITDFLKKFKLISHTTLYSLKALEQDGWLSFNEQVFLPATVMFTVSKESLYAFEK
                     ENPQLESCIKTLLRAYEGIFDQATAISEKVIAGLMRMPFDDVKAQLLLLKSAGIIEYQ
                     PQKDTPQLLMLRSRIKAEDISIDMAAYNKRKERFRLRMKQMAAYVKEEAECRSRIIGN
                     YFGDEKIKACGICDDCLRQKSAQLSQEEFEVLHHRIINMVKYESLHTKDLLQKLNSIK
                     KEKAWKVLEFLQAENKIEMDATGWVKLK"
     assembly_gap    12835..12870
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12871..13658)
                     /locus_tag="PK977_03200"
     CDS             complement(<12871..13658)
                     /locus_tag="PK977_03200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRF17141.1"
                     /translation="MPLSPFEILKRYWGYNAFRPLQEEIINSVLHGKDTLALMPTGGG
                     KSLCYQVPALVKPGLCLVISPLIALMKDQVENLRKKGITAYAIFSGMSRKEVINIFNV
                     ATESNCKFLYVSPERLETALFKEYLPGLDINLIAVDEAHCISQWGYDFRPPYLRIAAL
                     RKELPGVPMLALTASATPDVQNDICKKLEFTDKQVFRQSFERPNLSYSCFKVDSKINK
                     IIEVLQKVPGSSIVYCKSRKRTKEISELLRLQNISSDFYHAGLV"
     gene            complement(13721..15841)
                     /locus_tag="PK977_03205"
     CDS             complement(13721..15841)
                     /locus_tag="PK977_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRF17142.1"
                     /translation="MEQVQWKVDGMDCNTCALNIHKYLEKKGMKNIKVNFATGYVSFD
                     MNGVANEKELAKGINGLGYEVAEHKSEILPKKTAFLSTHLQRFWFCFPFTAVLMLHMI
                     PGLHIHWLMNHWVQLALTIPVYLVGMSFFGKSALKSIRNRMPNMNVLIAVGSTAAFVY
                     SLYGSLTGQAEQYMFYETAATIITLVFLGNYLEDTSIASTQRELNKLAKSQKVMANMI
                     AFDDEHKEQIFPVENTDLRVGDLVLIKSGEQVPIDCKILWGDVHVNEAIITGESLPVH
                     KKAKDILIGGSVITDGTVKAQVTAVWNETVLSGILKMVKEAQGEKPPVQLLADKISAI
                     FIPIVLVIAAATLVISWIVLKEFTPALMRSIAVLVIACPCAMGLATPAAIAVGLGRAA
                     KTGILFRNATSLETFKDIKQVVFDKTGTLTTGLFTIGNWQITNPSVNESEFKKIVFSL
                     EKYSNHPIAKCISEAWKSKEQILWKNIEEVKGLGMQAEDKEGNRFIAGSYKTVAGLTK
                     EDNHNVYILRNGHFLGWIDVKDEIRPEAKRVIDYLHSKNIKTILLSGDRLAKCNELAV
                     QLGIDTVIAEQTPEEKLNKIAELSAAAPTAMVGDGINDAPALTKATIGISMNEASQIA
                     VQSAQVILMNHGLKNLPTALGLGKHTYLTIKQNLFWAFAYNIVAIPVAAFGFLTPTFG
                     ALVMGMSDVVLAINSVRLFVKKVV"
     gene            15932..16357
                     /gene="tsaE"
                     /locus_tag="PK977_03210"
     CDS             15932..16357
                     /gene="tsaE"
                     /locus_tag="PK977_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRF17143.1"
                     /translation="MDLVFTLNTINEAAAQFWKAVGKHKVFAFHGPMGAGKTTFIHAL
                     GDAKGVKDVVGSPTFSIINEYVYDCEGTKRPLFHMDLYRLNSEEEAQRAGVEDALYSG
                     HLCLVEWPEKAPGIFPAETVHVYIELLDTQSRRLKIANI"
     gene            16423..>16632
                     /locus_tag="PK977_03215"
     CDS             16423..>16632
                     /locus_tag="PK977_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRF17144.1"
                     /translation="MSQQKPKISTSFSYETLEETLDIKPKSEGMMIGIPKEIAFQENR
                     IALTPDAVSVLISNGHQVMIEHNAGE"
     assembly_gap    16633..16664
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16665..17644
                     /locus_tag="PK977_03220"
     CDS             <16665..17644
                     /locus_tag="PK977_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRF17145.1"
                     /translation="SEAGAKIVYERAEVFKAPILVKSAPIIDEDLPLLQLNQIIISPI
                     HQSILKAEILEKMMEKRVTAISFENLKDDSESYPIVRSMSEIAGSAVMLIAAQYLSSA
                     NHGKGVLFGGISGIAPTKVIIIGAGIVGEYAARAALALGASVKVFDNNVYRLKRLQNN
                     IGQRMWTSVLEPRMLAKQLKTCEVAVGALGSETGRTPLVVTEEMVSNMRPGSVIIDVS
                     IDRGGCFETSEITSHEHPIFMKYGVIHYCVPNIPSGFARTASQAISNVLMPLLLEAGE
                     EGGFENLLWHKIHLRQGIYIFKGALTNFYLSQRFDLKYTDLNLLIASRR"
     assembly_gap    17759..17900
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17901..18722)
                     /locus_tag="PK977_03225"
     CDS             complement(<17901..18722)
                     /locus_tag="PK977_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF17146.1"
                     /translation="MKRIILAILLLTQIGAVAQKIPFSITIRFELITSPRYIYVFMND
                     ELRTDTIPYPESNVLIYSGTLSKPGTFQLATDSAVATQLLWIDNIPASVTFKEKKTRF
                     EKNELVITNINGSEDAIWLYNLGQPRQMPAKTYSPQAIEKRKDSSNVEVATFYYNFID
                     SLIKKRPASLVIPYYIQYYRYLLGNENTQILYNRLNAEQQNYDIAKEIIKFLNQNVSL
                     QRGALIENFTMKQPNGKQLSLYDVSGNYILLDFWASWCGPCRVANTSLVKLYEKFN"
BASE COUNT         5178 a         4004 c         4002 g         5252 t
ORIGIN      
        1 ttcagtattg attataacag gagttgggtg ttttgcaatc aatgcaatac tgtcaataat
       61 atcggggtca ttagcaccca ccgtgtagtt atctattaca aaatcccatt catcttcaaa
      121 aacgggttga ccggttttat ccaggtgaac ctcgccttta ataacagttt catcatcaat
      181 cctggaggct ttaccatcca gcatcatttt acccccgccc ggacagttaa aatcagccct
      241 tgataacagg ggcatttttc cgggnnnnnn nnnntgtgcc aatactaaag cgacccgatt
      301 tttcttccag taactggtca tcccaccaaa cggcctcatt agtacgggca tcaaaatatc
      361 ctttgataga atacctcctg taattgcccg gggattcata ataatatgat gtaccagtca
      421 ggctgtcacc tttctgaata attttgactt ctactttttg ccggtttatc cggcccttcc
      481 aggtgcctgt aatcatttga ctgtaactat cagcagacag caacaagcca agcaggccaa
      541 aggcaacaaa aatgcgtttt tctggcttca atgaatgatg tttttttaag gaatatgtta
      601 ctgcttgcat aaaacggcaa ggcctgcctg ttagtatccc gaaatgatag tcattcaatc
      661 ttttaccatt cttttccgaa aaaaacttac gaaggaaatt taccatgtag taaaagaaat
      721 tttcttacat tcgggacgcc aagtgggtat atcccctggc ttaactatta aactaactgc
      781 gtatgcgttt tctttacccg caaacggaaa atgtacatca attgtccatt tctccggttt
      841 taagaaaaat ttcaatccgt tttattttct cttttgcatt aatggctttt agtgcaagcc
      901 tgtttgccca aaatccaggg aaggcaaact ataaactcat caatgaaaat gatgggagtg
      961 ttcgttatga aatttccaat ggaggaaaag ttgttttcac tgaaaagatt ggaaatatca
     1021 gtgataaccc gcaggccata agtgcttatg ctgataagaa agcaaaggct gccttaagag
     1081 cttatcagca aattggcaat tatcctcagc aggaaatagc tgaccgcata aaaaaacaaa
     1141 tggcaaacga cccggagatt gcacagagaa ttgttccgga aggtgcctgt acttataata
     1201 gcactattga tggaacggat gcttcaatgg cagatcggct attacgtccg gctggaacgc
     1261 cggcaagttg tgcagtaccc gttgcctgcc ccggaccttt tggtggtgta ggcccctatt
     1321 tttatgaaac atataccttg acaaactcaa ccggtaccaa tcagtgtgta acagttaata
     1381 tcaattgggt ttctggtaca ggaacagctg tgcatgtaac tgcctattcc ggtagtttta
     1441 atccggctag tttgtgtact aattttttag ctgaccctct tacaagtgct gtttctggaa
     1501 ctccccagtc tttttcattt gatgttgcaa atggagctac ggtagtactg gtggctacaa
     1561 gtcccaatgg aggcgatgtt acttattcta tgaccgttaa tggtctctac gctgaccctg
     1621 ttatcgtatc tcaaccgctt gcacaatcat tctgtggtag tggtacacct actttctctg
     1681 taggagcaac aggtgttggt ttgggatacc agtgggaaag aagtactgat ggaggtgcta
     1741 catatctccc aatcgcagga gccaactcat cttcatattc tcccgggcct gtaacaatag
     1801 cagcaaatgg ctatttgtac aggtgtgtgg ttacaacaag ttcaccgggc tgctctggtt
     1861 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aacacaactg gttctactac catcacaggt
     1981 actgccagcg gtggtacagt gggtggtact ggtactttca gttcaggtac aatcaatctg
     2041 gcaatcgttg ataacggttc tgtcaatagt acaattgcat taccagcaat aaacataaca
     2101 aacgcaaatg atctgaaagt acggattaat gcaaaccata catgggttgg ggatttgact
     2161 ttccaattaa ccagtccttg tggcgtaact tatttgtttg acaggccagg cctgaaccct
     2221 ccgggtacat gctgtggcag ttcatctgat ctggcaggtg tgtatgaatt tgatctcgca
     2281 gcggcaacag cttttcctga agcccctgcc agccccgtac ctacaggcat atacaggcca
     2341 actgatgcat ttggcgctcc gcataactgg gctggtgctg ctttcccctg cagtgcaggc
     2401 ggaaactgga ctttatccgt agcggatgga gccgttggtg atgtagggac attggttgat
     2461 tgggcaatca tagtaccagg cggttacaca catacattaa ccggtcccgg aacaattacg
     2521 caaaacccat caagcggacc taataatgca acaggaagct tcactgttag cggtctcgcc
     2581 ccgggtacat ataattatac attcacttct acggatgcag ttggatgttc tgttagtact
     2641 aacgtatctg ttcaggtgaa cccgataccg agcctgacta tttcaccttc atctgcagct
     2701 atttgtcctg gtggtattgt gcagttaaca gcaaccggaa ctggtggttc attgactaca
     2761 ttcagttctg cggctgcaat tacaattcct gccagcggtg tagctacacc ttacccttct
     2821 accattaatg taagtggttt gccaactggt ggcgtgaatg tagcgactgt tgtgttaaac
     2881 aacttctctc atactttccc ggatgatgtt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagatgtt ggtggcggaa ccgatgctgt
     3001 taaccttact tatacttttg atgatgcagc agcttcattg atggctgatg ctgccttgaa
     3061 tccgacagga acatacagac caactaatat tggggcaggc gataattatc ctgcacccgg
     3121 acctttgaca gcaccaacgg caacaacttt agccactttt acaggtaacc ccaatggtaa
     3181 ctggagcctg tatgttgtgg atgatctggt aggtgacgca ggaagcatca gtggcggatg
     3241 gagcatcaca ttcaacgctc ctccaccagc taatgccgta tttactccgc agaacggatt
     3301 atttaataat gcaggagcta cgataccgta tactggtact gcacaaagta cggtatgggc
     3361 atctcctgta gctacaacaa cgtacacggc tacaacaact gtaaacggtt gtacatctgc
     3421 gcctgtaaat gtgactataa ctgttaatcc tgttactacg atcaccacac agccagctaa
     3481 ctcggctatt gtttgtgcag gaagcgccgc cacattcagt gtagtagctg caggtacagc
     3541 accattgact taccagtggc aaataagcac tactggtgca ggtggcccat ggaccaacct
     3601 caccaatact gctccttatt ctggtgtaac aactgctaca ttaactatta acccggttac
     3661 tgcaccaatg aatggtaatt tatacaggtg tgtggtaaca ggaggttgtg gtactgcaac
     3721 aagtaatgca ggtactctga cagttgacca gttatctcat acaggagtag cagctaaccc
     3781 cgctgtaaca tgcagccccg gttctactac tatcgctggt acagcagtga acggatcttt
     3841 tgccgtgagt ttaggtactt taggttcttc cggaacaatc aatcttccta taccggatgg
     3901 taatcctaca ggagtaaact ctactattgt actgcctgca aattcaattg caaatgcaaa
     3961 caacctaagg gtaagaataa atacaacaca tacatgggta ggtgatgtta gatttacact
     4021 taccagccca tgcggaacaa ccttcctgtt tgacaggcct ggagtacccg cctccacatt
     4081 cggaaattcg gcggatttgt caggtgttta cacatttgat gtaactgctg ctacaataat
     4141 acctgaaacc ggaggtggtg taattgctgc aggatcatat cgtccgtctg acgctcctgg
     4201 taacccgcat aactgggcag gcttaacttt cccttgtgct actgcaggta actgggtact
     4261 taatgcatcc gatgcagcag cgggcgatgc gggtactttg gtatcatggg aaattattgg
     4321 ccctgttccg gcaaactata cgcatacgct tacaggaccc ggaactatcg gtgcgccggt
     4381 attatccgga gcaaataatg ccacagcttc attccctgta agcaatattc ctgcgggtaa
     4441 ccatacttat gtgttcactt ctactgacgg aaacggctgt tctgtaagta ccaatgtgaa
     4501 tgtattggtg aacccgacac ctgttgtaac tattgctccg gctgctccgg taatttgtgc
     4561 aggtaatatt cagcagttga ctgcaactgc tgttccgggt gttacacaga ctttcaactc
     4621 tgcaacactc aatcttgcta tcccggataa taatccggca ggtgttacta caccggctat
     4681 tgtattacca gctggtattt caattactaa cgcagctgat cttaaagtaa gaattaatgc
     4741 taaccataca tgggttgggg atttgatttt tagagtaacc agtccatgtg gaacaacatt
     4801 cctctttgac agaccaggtg ttccggcaac tacatttgga aattctcaaa acctgggtgg
     4861 ggtttataca ttcgatcttt cagcagctac tattttgcca gaaaccaact ccgggcctac
     4921 aactattccg gctggatctt acctgccttc aaatactgcc ggagcagcgc atacttgggc
     4981 tggtgctacg ttcccttgtg ctgcaacggg taactggaca ctaactatgt ctgataatgg
     5041 tggtggtgat acaggttcac taatagattg gggtataatt gtgactacac cttcaccggt
     5101 tgtattcagt ccaacaacta acctgtatac aaatgctgca gccactatac cttataccgg
     5161 aacaccggtt actacggtat gggctgcacc tccgactacc actacatata ctgccacagc
     5221 aacggtagca ggttgtactt ctgctcctgc cactgtaact gtaacagtga atcagttgcc
     5281 tgctatcaca gtacagccac aggcattgtc agcacctgtt tgccctggct ttaatgtgaa
     5341 ctattcagtg actgcaacag gtacaggctt aacctaccag tggcagatca gtacggataa
     5401 cggaacaaca tggaccaacc tggttaataa tggtacacag tatgcaggtg taactacatc
     5461 tacccttact ttactgaatg tacagacaac acagaatggt cacaggttcc gtgttattgt
     5521 ttctggtgtt tgtcctccgc cagcaacatc tagttcagtt acattggtag tggctactcc
     5581 gccgactatt actacacagc ctgcaaacct taccgtttgt gccggtgtta atgcaacgtt
     5641 cactgttgct gcaaccggag ttcctgctcc gaatatctat cagtggcagg taagcactaa
     5701 tggtggcgtt acctggacta accttacaac aggtggttca tacactccaa cattgactgt
     5761 gtctccgaca actacggcat tgaatggaaa ccgttacagg gtgattgtaa ccaacagttg
     5821 cggacagaat gtgacttcta atgcagctat actcactgtg aacccgctgc cagtagtaag
     5881 cgctactgac ctctggaacc gcagaatctg tatcagtgat acgctggttc cgctggtggg
     5941 tacaccaaca ggaggtagct ggagtggcat tggagtgtct ggtttcaatt ttgtaccaac
     6001 tgcaacagcg ataggtacat acacacttac ctacaccttt accaacagct ttggatgtac
     6061 tgctacagat actactaagg cgattgtggt tgactgtccg gaaagaatca ggttgctgag
     6121 agataatgcg gtaattctgt tcccgaatcc gaatggcggt cagtttaata tcaggatcaa
     6181 tagtgtgttg tataattatc ttggtatgaa tgtttacaat tctgccggtc agttaataag
     6241 tcagaaaaca ttcagtggtt tggcatacgg tagggttgtg ccaattgatc tgacacactt
     6301 gccatctggt gtttatatgg ttaagttctt ctacgatgat ggtatcagaa catctgagaa
     6361 gaccttccct gtaattatcg gaagacaata agcttagagt tatcaaatca tacaacaccc
     6421 cggttttccg gggtgttttt tttgcagtag catattctaa ctttaaaaaa atgaaccagg
     6481 ctttagcttt taaaaatatt agtaagacat ccggtatctc atttaaccgt gttattcctt
     6541 tcgaccctgt aaaggataaa cttgttttat tggattttac agcagctaac gaagaactgg
     6601 agccgggtat attgaatgat ataacccggt tcagccaatg ggttaataat aaactggagc
     6661 atagccatgc acggtatgga attggtggtt atgcagaaca caggacagtg tacagtgcca
     6721 gtaaagtatt tgatggaaat aatgatggcg aagaaccaag aaggttgcac ttgggaacag
     6781 atatatgggg aaaaccaaat actgcagtaa tagctccctt agatggtata gtgcacagct
     6841 ttgcttttaa taaccgcttt ggtgattatg gcgccactat tattctttcg cataacctgc
     6901 agggctttac tttctttacc ctatatggcc atttaagcct caattcaatc aaaaatatcc
     6961 gcgaaggaca acggatttcc gccggtgaaa tatttgcaga attcggaatt cctgcagaga
     7021 atggccaatg gccgccacat cttcattttc agataatact ggatattggc aactggcatg
     7081 gagattatcc tggtgtatgc aaattctcag aaaaggaaaa atggctggct aacagtcccg
     7141 atccggacat cattctgcaa ttgaaccaat acctgtagca gcaattatgc ttttaataac
     7201 ttaaaggcat gtgaccactg gttacggata aaaccgggaa tgcaccaaag tatacatagc
     7261 ggttcgatag cccgggcagc ttctacccgc cccatatcct ctgtttccca tccaaaagat
     7321 gtaaggatga ccgatacttt ttctttagct tcagcatcat taccgcaaat aaacattgta
     7381 ggcttaccct ctttgaatga cggtttgtac ataaacgcat tacccacaca gctgaatgct
     7441 ttaaccacct ttgcatcggg tatcatgttt tgtattcttt ccattagaga atcttccagt
     7501 gtggtaaaaa attttaatac gccgttatca ggaggtactg cagccagcgg attagttgtg
     7561 tcaatcacaa tcttttcaaa gaaatgttct ttgcccgcca gtgaaatggc atcttcaaca
     7621 acaagtccgc ttacagccaa aactattact tcaccaaatt gtgctgtttc ctggaaagag
     7681 cctaacaacc cgcttctgtt ctcctgttgc cattttacca cttcttcctt tgagatgttc
     7741 ctggtgccca gcatcacctg gtgcccttct tttaaaaaac cggttgccag cacacggcca
     7801 acctggccag agccaattat acccaccttc ataaaagtgt tttttaatga ttagttttac
     7861 aaagtttaca aaaaatattc atggcaaacc ctaacctgaa ttcggataaa gagagtttaa
     7921 gttctgcgga tgtggcattt gagaattcta tcaggccatc acaaataaat gatttttccg
     7981 ggcaggacca gatcattgaa aacctgcgga tttttatcaa agctgctaaa ataagaggag
     8041 aagcactgga ccatgtttta tttcatggtc ctccgggttt gggtaaaaca accttatcaa
     8101 gaattgttgc caatgaactg ggagtaaaca tcaaagaaac atcgggtcct gtaatcgaaa
     8161 agccgggtga tcttgccgga cttttaacaa gtttagaagc gaatgatgtt ttgtttatag
     8221 atgagataca ccggctcagt acagttgtag aagaatactt gtatgcagca atggaagatt
     8281 atcggataga tataatgatc gacagcggcc ccaatgcccg cagcattcaa ataaacctca
     8341 acccctttac acttattggg gcaactacca gaagtggctt attgacagcg ccacttttat
     8401 cccggtttgc cataaaatca aggttagaat attataaatc cggggtgctg aataaaatca
     8461 tacaacgttc agccggcatt ttaggaacaa aaatttctaa agatgccatt gaagaaattg
     8521 gccggagaag ccggggcact ccccgtattg ccaatggttt gctaaggagg gtgagagact
     8581 ttgcacaagt actgaatgat gggattattg acctgggcat tactcaacat gcattaaagg
     8641 cattgaatgt agatgaacat ggattggatg atatggataa tcgaatcctt tctactatca
     8701 tcgataagtt caaaggtggt cctgttggca tcaccacaat agccaccgca gtgggcgagg
     8761 aagcaggaac actggaagaa gtttatgaac cattcctcat ccaggaaggt tttatgcagc
     8821 gtacaccaag aggaagagaa gttacagcaa aagcctatga gcatttggga aaaacaaaac
     8881 ccgggtcagc cggaacattg ttttcctaaa caaaaaggat tatgagttgc cccataatcc
     8941 ttataatccc ttttatggca gttctattgc actactaacc ggaagccgtt tccgtgaatg
     9001 tttacaatct caattttcgg atcgtctttc aggtatttgc ggagtttggc aatatacaca
     9061 tccatactac ggccgttaaa gtaagtatcg ctgccccaga tttttttcag tgcagcttct
     9121 cttggtaata aatcattcag gtgctcggcc agcattttca ataactcatt ttctttcggg
     9181 ctcagggtct gggttacacc atcatgaatt agctggcgca atttgggatt gaaatgatag
     9241 ctggccaggt catactcttt gttttctgat tctttgttct gctcttcatt ccttttcagg
     9301 atggccttta ttttcaacag caatacttcg ctgtcaaaag gcttggttat gtaatcgtca
     9361 gcgccgagtt tatagccttg tatcacatct tccttcatcg ttttggcgct gagaaagaac
     9421 aggggaatgt caggatcaac atctcttatt tcttcagcca gggtgaagcc gtccatattg
     9481 ggcatcatca catccagcag gcagagctca aatttttccc gctgaaaagc ggccaggcca
     9541 aggcggccat ctctttccag ggttacatca taatcgttca gttcgagata attcttcaac
     9601 acacttccca ggttcggatc atcttcgcac aacagaattt taggtttttt cgcttccatt
     9661 tctagtggtt ttatattggt gacaaataaa gttaaagcat ttcgggggtt caagtatcgg
     9721 tgctgaatat tttgttaaaa caccgtggtt taaaaccctt tgctgtctac ttttgcttta
     9781 cataataaaa aactgtgcca tgcatataac acctgataac aggctaaaaa aattaatcgt
     9841 cattggcgac cggttattga taaagccaac ccggccggat gaacgtactg ccagtggctt
     9901 gtacctgcca ccgggtgtgc aggaaaagga gaaagtacag cagggatatg tgataaaagc
     9961 tggtcccggc tatgtgattc caatgccggt cgaagaggaa agctggaagg gcgaggaaga
    10021 acaggtaaaa tatgtgccat tacaggccag agaaggagac cttgcgatat tcctgctcag
    10081 cggtgccacc gaagtgatgt atgagaacga aaaatacttt atcgttcctc aaagtgctat
    10141 cttaatgctg gagagagaag aagagttata gattattcaa ttcatcaagt ttaaaataaa
    10201 aacaatggca gacagtacaa aattcctgga aatcgtcaag gcaggatata cattcaaagg
    10261 tgaatcgttc aagattggtt gtgccatgct gaatggcgag gtagtgccgg gaactgatgt
    10321 gttgttgcct ttcaaaacac tgaacaggca tggcttaatt gccggcgcaa caggtaccgg
    10381 caaaacaaag acgcttcaag tacttgccga aggattaagt gatgccagta tccctgtatt
    10441 gctgatggat attaaaggcg atttaagcgg aattgctgct gccggcacgg tgaatgataa
    10501 aataacagat cgatgtcaga agctgaatat tgaatttaaa ccaacagctt accctgttga
    10561 cttactgact ttaagtgaac aaaaaggggc aagacttcgg gctacggtca gtgagtttgg
    10621 cccggtgttg ttatcaaaaa ttttgggact taatgataca cagggcggtt ttgtagctat
    10681 gatattcaaa tactgcgacg ataataaact gccgctgctt gatttgaaag atttcattaa
    10741 agtactgcaa tatgtcagtg acgagggaaa agccgagttg gaaaagacct atggtaaaat
    10801 atcaactact tctactggaa cgatactcag aaaggtaatt gagttgcagc aacaaggcgc
    10861 cgatctgttt tttggagaaa aaagttttga agtagaggac ctgatgcgca tcagtgatga
    10921 tggccgtggt atgatttcaa ttgttagggt aactgacctg caggacagac ctaaactgtt
    10981 ttctacattc atgttgcagc tgctggcaga attgtatgcc acctgtcctg aggaaggtga
    11041 tatggacaag ccaaagctgg tcatgtttat tgatgaagcc catcttattt tccaggaggc
    11101 gaatgatgca ttattacaac aaatagagac gatcatcaaa ctgatacgtt ccaaagggat
    11161 tggaatttat ttctgtacgc aaaaccccat ggatgtgccg gctgatgtgc tggcgcaatt
    11221 gggattgaaa gtgcagcatg ctttacgggc atttacagct gcagacagaa aagtgatcaa
    11281 acaaacagcc gaaaactacc cggagactga tttttataaa acagaagacc tgatcaccca
    11341 gttaggaacc ggagaggccc tggtgacaat attgaatgaa aaaggtattc ccactcccct
    11401 ggtgcattgt atgataaggc cgccacaaag ccgcatggat attttatcgg atatggaaat
    11461 tgatgctttg gtaagcaagt ctaaaatcgc atcaaaatat aaccaggtaa ttgataatgt
    11521 gagtgcatat gagatactaa cggaaaaact agaagaagct tcgaagaaag aggaagaaca
    11581 aaagcaaact gaacaaaaag aagagaagaa aactactaca aaaaaagaaa agtccgtttt
    11641 tgatgatcct accatcagaa gcatgacaag aactgcgggg aacactatta tacgttcatt
    11701 acttggtgta ctgggtttgg gaggacgcag ccgtagaaaa agtttatttt aattttaccc
    11761 accctgtagc atccatttca attttatttt cagcctgtag aaactctaat actttccagg
    11821 ctttttcttt tttgatgcta tttaattttt gcagcaggtc ttttgtatgc aacgattcat
    11881 atttcaccat attgattatg cggtgatgca atacttcaaa ttcttcttgg cttagctgcg
    11941 ctgatttctg tcgaagacaa tcatcacaaa taccacaagc ctttattttt tcatcgccga
    12001 aataattgcc aatgatacgg ctgcggcatt cggcttcttc ctttacatag gcggccatct
    12061 gcttcatgcg caggcggaat ctttctttac gtttgttata agcagccata tcaatggata
    12121 tgtcttctgc ttttatcctg ctgcggagca tcagtagttg tggtgtatct ttttgtggtt
    12181 ggtattcaat aatgccagca cttttcagca gcaataattg tgctttcaca tcatcaaaag
    12241 gcatcctcat caacccggct attacttttt ctgaaatagc tgttgcctgg tcgaaaatac
    12301 cttcataagc cctaagcaaa gttttgatac aggattccag ttgcggattc tctttttcaa
    12361 aagcataaag actttctttg gaaacggtga acataacagt ggccggtaga aacacctgtt
    12421 cattaaagga caaccagcca tcctgctcca gtgcttttaa cgaatacaac gttgtatggc
    12481 tgataagctt aaattttttc aggaagtctg taatgtcaaa atcatagtat tgactctcac
    12541 cgcttccact gggtatttgc aggtaattgg ccacggactg gtaaacattc cggatatcat
    12601 ccaggccggg aaaacgcaga tcggccattt cttccagttc atgcagatcc cggttgtcat
    12661 atagcaaaac tgcataactg gttttcccat ccctgcctgc tcttcctgcc tcctggtaat
    12721 aattttccag gcaatccggt acatcagcat gaatgactgt ccttacatcc ggcttatcaa
    12781 tacccatacc aaaggcagtg gtacaaacga taacccttgt tttgttacca atccnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtacaagtc ctgcatgata gaaatcggaa
    12901 gaaatatttt gcagccttaa tagttcactg atctctttgg ttcgtttccg gcttttgcaa
    12961 tacacgatgg agctgcccgg aaccttttgt aatacttcaa taatcttatt gatcttggaa
    13021 tctactttga aacagctgta cgaaagatta ggccgttcaa acgattgacg aaatacctgc
    13081 ttatcagtaa actccagttt tttgcaaata tcattttgca catcaggagt cgctgaagcg
    13141 gtgagggcaa gcatagggac accgggaagt tcttttcgaa gggccgctat gcgcaaatac
    13201 ggaggccgga aatcataacc ccattgggag atacaatgtg cctcatctac cgcaatgaga
    13261 tttatgtcca ggcccggtaa gtattcttta aaaagagccg tctccagcct ttccggagaa
    13321 acgtataaaa atttacaatt actttcagtt gctacattaa aaatgttgat cacttctttc
    13381 cggctcatac cagaaaaaat ggcataagct gtaatcccct ttttccgaag attttctacc
    13441 tggtctttca ttagtgcaat aaggggagat attactaagc ataaaccggg cttcactagt
    13501 gccggtacct ggtagcacag cgattttccg ccacccgttg gcatcaaagc aagagtatct
    13561 tttccatgta aaacagaatt gattatctct tcctgtaatg gacggaaggc attataaccc
    13621 cagtaacgtt tcagtatttc aaatggagat aacggcatgt tcctatttca tggttcatgg
    13681 aagaaatcta tgaaccatga actataagct atgaactaaa ttacactact ttctttacaa
    13741 aaagccttac tgagttaatg gccaatacca catcactcat acccattacc aatgcaccga
    13801 aagttggggt aagaaagcca aaggcagcaa caggaatagc tactatatta tacgcaaagg
    13861 cccagaataa attttgcttg atggtcaggt aagtatgttt tcccaatcct aatgctgtcg
    13921 gtaaattttt gagaccatga ttcatcaata taacctgtgc actttgcaca gcgatctgcg
    13981 aagcctcatt cattgaaata ccaattgtgg ctttggtcaa tgccggggca tcgttaatgc
    14041 cgtctcccac catggcagtg ggtgcggcag cacttagctc agcaatctta tttaatttct
    14101 cttccggtgt ttgctctgca attaccgtat caattcccag ttgtacagcc agctcattac
    14161 acttggccag cctgtcaccg ctaaggagga ttgtcttgat atttttgctg tgcaggtaat
    14221 caatcaccct tttggcctca ggcctgattt catccttcac atcaatccag cccagaaaat
    14281 ggccgttacg cagtatgtaa acattatgat tgtcttcttt tgtcaggccg gcaacagttt
    14341 tataggatcc ggctataaac cggttacctt ccttatcttc tgcctgcata ccgaggcctt
    14401 ttacttcctc aatgttcttc caaagaatct gttctttgct tttccatgct tcagagatac
    14461 acttcgcaat ggggtgattg gaatatttct ctaatgaaaa aacaattttt ttgaattcac
    14521 tctcattgac agatggatta gtgatctgcc agttgccgat agtaaataaa ccggtggtaa
    14581 gtgttcctgt tttatcaaac actacctgtt tgatatcctt gaatgtttcc aggctggtgg
    14641 catttctgaa aagaatacct gttttggcgg cacggccaag ccctacagca atggcagcag
    14701 gagtagctaa tcccatagca caggggcagg caataaccag gactgcaatg ctgcgcatca
    14761 gggctggtgt aaattctttc aatacgatcc agctaatcac cagtgtggcc gcggcgataa
    14821 cgagtactat tggtatgaat atagcactga ttttatcagc cagtaattgt acaggcggtt
    14881 tttctccctg ggcttctttc accattttta aaatgccgga aagaactgtt tcattccata
    14941 cagccgtaac ctgtgctttc actgtcccgt cggtgatcac actgccgcca atgagtatat
    15001 cttttgcttt tttatgaacc ggtaaacttt ctccggttat gatcgcttca tttacatgca
    15061 catcacccca tagtattttg cagtcgatcg gaacctgttc accgcttttt atcaatacaa
    15121 gatcacctac ccgcaggtct gtattttcta cgggaaaaat ttgttcctta tgctcatcat
    15181 caaatgcgat catattggcc atcacttttt gtgatttggc cagtttattc agttcccttt
    15241 gtgtggaagc aatagaagtg tcttcgaggt aatttcccag gaatacaagt gtaataatgg
    15301 tggctgccgt ttcataaaac atatattgtt cagcctgccc tgtgagtgat ccataaagac
    15361 tgtacacaaa tgctgcagtg gatccaacag cgatcagcac attcatattg ggcatccggt
    15421 tacggatact tttcaatgca cttttgccga agaaactcat gcccacgaga taaacaggaa
    15481 ttgtcaatgc cagttgcacc caatgattca tcagccagtg aatgtgcagg cccggtatca
    15541 tatgcagcat cagtactgct gtgaagggaa aacaaaacca aaaacgctgc aggtgggtgg
    15601 aaagaaaagc tgttttcttc ggcaggattt ctgatttatg ctcagctact tcatagccaa
    15661 gcccgttaat gccctttgcc aattcctttt cgttggcaac accattcata tcgaagctta
    15721 cgtaccctgt ggcaaaattc actttgatat tcttcatacc ttttttctcc aggtatttat
    15781 gaatattcag tgcacaggtg ttacagtcca ttccgtctac cttccattgt acctgttcca
    15841 tactgcaaat ttaccaaata acggagggag gctttttgct gaaacgggaa tatttgagca
    15901 acaaggttgc tattccggct aaatttgcaa catggattta gtatttacac tcaatactat
    15961 aaatgaagca gctgcccaat tctggaaagc agttggtaag cataaggtgt ttgcctttca
    16021 tgggccgatg ggtgcgggaa aaaccacctt cattcatgcc cttggtgatg ccaagggggt
    16081 gaaagatgta gtgggcagcc ccactttctc aattattaat gaatatgtgt acgactgtga
    16141 gggaaccaaa cgtcccttat ttcatatgga tctttaccgc ctgaacagtg aagaggaggc
    16201 gcaaagagcc ggagtggaag atgcattata cagcggtcac ctttgcctgg tggaatggcc
    16261 ggagaaagct ccgggtattt ttcctgccga aacagttcat gtatatattg agctgctcga
    16321 tacgcaaagc aggagactga aaatcgctaa tatttaggta catttgaata tgtgtcacag
    16381 ccggatgatt agtcccgggt tacctaacca gcaaccagct tcatgagcca gcaaaaacct
    16441 aaaataagta cttctttcag ctacgaaaca ttggaggaaa ccctcgacat caagcccaag
    16501 tcagagggaa tgatgattgg tattcccaaa gaaattgcat tccaggaaaa ccgtattgca
    16561 ttaacacccg atgccgtgag tgtgctgatc agtaatggtc accaggtgat gatcgaacat
    16621 aatgccggcg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacagtg aagccggtgc
    16681 aaagatcgtt tacgaaaggg ccgaggtttt taaggcaccc atccttgtaa aaagtgcacc
    16741 tatcattgat gaagacctgc ccttactgca gttgaaccag atcatcattt cacccataca
    16801 ccagtcaatc ctcaaagcgg agatactgga aaaaatgatg gaaaaaagag tgacggctat
    16861 ttcatttgag aatctgaaag atgacagcga atcttacccc atcgtaagaa gcatgagtga
    16921 aattgccggt agcgccgtca tgctgatagc agcgcagtat ctcagttctg ccaaccatgg
    16981 taaaggggta ctgttcggtg gtatctccgg tattgcgccc acaaaagtta ttatcatcgg
    17041 tgccggcatc gtaggggagt atgccgcaag ggctgccctt gcattaggtg catcggtaaa
    17101 agtgtttgac aataatgtct atcgcttaaa aagattgcag aataatattg gtcagcggat
    17161 gtggacctct gtactggagc cccggatgct ggccaagcag ttaaagacct gcgaagtggc
    17221 cgtgggtgcc cttggaagcg aaacaggacg tacaccctta gtggtaacag aagaaatggt
    17281 cagtaatatg cgacccggtt cagtgattat tgatgtgagc attgaccgcg gcggttgttt
    17341 tgaaacttcg gagatcactt cgcatgaaca ccccatcttt atgaaatatg gggtgataca
    17401 ttattgtgtt cccaatattc catccggttt tgcccgtaca gcatcacagg caatcagtaa
    17461 tgtactgatg cctctattac tggaagcagg agaggaagga ggatttgaaa acctgctctg
    17521 gcataagata cacctgcgcc agggtattta tatcttcaaa ggcgccctca ccaatttcta
    17581 ccttagccag cgcttcgatc tgaagtacac cgacctgaac ctgcttatcg ccagcaggag
    17641 atagaacact attttaaaag attagaaagc agcggatcca gttggctggc agtaaactgg
    17701 tttgcaatta ctttgtaatt actgtccagc agaatggtga agggaatgga agaaatttnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn gttgaatttc tcgtatagct tgactagact ggtgtttgca
    17941 actctgcaag ggccgcacca gctggcccaa aagtcaagta aaatataatt tccgctaaca
    18001 tcatatagtg aaagttgttt cccattgggc tgtttcatgg taaaattttc gatcaatgca
    18061 cctctttgta atgaaacatt ttgattcagg aactttatta tttccttcgc aatatcgtag
    18121 ttctgctgtt cggcatttag acggttataa aggatctggg tattttcatt tcccagtaag
    18181 tatctataat attgaatata atatggaata accaaggaag caggccgctt cttaatgagg
    18241 ctgtctataa aattgtagta aaatgtggcc acttcaacat tcgatgagtc ctttcttttt
    18301 tcgatagctt gtggcgaata ggttttagca ggcatttgcc taggctgacc taagttgtat
    18361 aaccatatcg catcctccga accattgatg tttgttatta ccagttcgtt tttttcaaac
    18421 cttgtttttt tttctttaaa tgttaccgaa gctgggatat tgtctatcca aagcaattgc
    18481 gtagcaactg cggaatctgt agccagttga aaggtacccg gttttgatag ggtgcctgaa
    18541 taaatcaaaa cattcgattc cgggtaggga atggtatcag tgcgcaactc gtcgttcatg
    18601 aaaacatata tgtacctggg agaagtaatg agttcaaacc ggatagtaat ggaaaagggt
    18661 atcttctggg caacagcccc gatctgtgtt agcagcagta tggccagtat gattcttttc
    18721 aaggatataa atgtaaaaaa aactgatgca ggttagctgc cggcaaatga ggagcctgcc
    18781 aaaattttgt tatcttactc gctg
//
LOCUS       DAORLS010000022        18730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1584_length_18730_cov_1.961124, whole genome shotgun sequence.
ACCESSION   DAORLS010000022 DAORLS010000000
VERSION     DAORLS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18730
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1584_length_18730_cov_1.961124"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>365
                     /gene="secA"
                     /locus_tag="PK977_03230"
     CDS             <1..>365
                     /gene="secA"
                     /locus_tag="PK977_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631286.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRF17147.1"
                     /translation="NAVYEKRNHALFGERLALDIDTSFSMVAEGLVSSFREQEDYEGF
                     KMACIVNFGLDTTIEEEEFKKGDLNTLTDRIYSEATEKYNRHKEELQKQAIPVFKNIR
                     LTQGSHIENVIVPFTDGRKG"
     assembly_gap    366..414
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <415..>635
                     /locus_tag="PK977_03235"
     CDS             <415..>635
                     /locus_tag="PK977_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17148.1"
                     /translation="KTLQSNAVELTNALEKTITLAVIDDAWKEHLRAMDDLKQSVQTA
                     YLEQKDPLVIYKVEAYNQFRNMNAEVNKD"
     assembly_gap    636..768
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    988..1134
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1135..1446
                     /locus_tag="PK977_03240"
     CDS             <1135..1446
                     /locus_tag="PK977_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17149.1"
                     /translation="QPTTTEPTTTPTTTEPAPTSTNADGVPSFSDPEVQKFANDYAAF
                     VKEYKEGMKDPAKLSALAQKMQDWSKRATEIGTKLATSPDEAKKWTDWTMAIAKELMP
                     Q"
     gene            1540..>1754
                     /locus_tag="PK977_03245"
     CDS             1540..>1754
                     /locus_tag="PK977_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17150.1"
                     /translation="MYKVTKKKVHGLLHPEIVGDKHWDKIINIFIISLIILNVIAVML
                     ETVSSLHEKYADFFHYFDVVSVIIFSI"
     assembly_gap    1755..1789
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1790..>2119
                     /locus_tag="PK977_03250"
     CDS             <1790..>2119
                     /locus_tag="PK977_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRF17151.1"
                     /translation="NHEEKYKGSLKGRLRYMLSPGALVDLLAILPFYLHAIIGFDLRM
                     LRILRLMRFFRLFRLTAYMKSAHMISNVFKKRINELALSLVLVIFLIIIASSAVYFAE
                     HQHIENRA"
     assembly_gap    2120..2134
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2135..2415
                     /locus_tag="PK977_03255"
     CDS             <2135..2415
                     /locus_tag="PK977_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRF17152.1"
                     /translation="KFTSIPATIWWAVVTLTTTGYGDMYPMTYLGKAMAMVIMLTGVA
                     FFALPAGIITAGFLEEFRMNRIKKTHRCPNCGEQLELDDNNHAHRTDP"
     gene            complement(2466..>2801)
                     /locus_tag="PK977_03260"
     CDS             complement(2466..>2801)
                     /locus_tag="PK977_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF17153.1"
                     /translation="FSISDDDSQTRGRNLPFNAGTAAAYGLTKEEALAAITLNAAKIM
                     GVADKTGSIEAGKDANIVVSDGDILDMRTSNVTDAFIQGRKIDLTDKHKLLNERFEQK
                     YEIKGKKGF"
     assembly_gap    2802..2834
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2835..3800)
                     /locus_tag="PK977_03265"
     CDS             complement(<2835..3800)
                     /locus_tag="PK977_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF17154.1"
                     /translation="MRKIFLIMTTCFTLAATAQETVLPAKPQKGIIYIKNATIHVGNG
                     KVIENGTIKIKDGKIEEVGASLPVPADDVSVYDVKGKHVYPGLILPTSSLGLVEISAN
                     RATTDVREIGDLNPNVRAIVAYNTDSRVINTLRSNGILLANVIPQGSLLTGSSSVVQL
                     DAWNWEDAAYKTDAGIHLNLPSLMARSGGFRGGGFGAGPSASQTDPVKEGLEQIEKVK
                     VFFREAKAYQAEGKHEETNLKFEAVKGLFEKKQKLYVNANTVKQILVALDFVKEFGVD
                     MVIVGGSDSYQVADLLKKHNVSVILQQPHSLPTATDDDVDQPYKTA"
     assembly_gap    3931..3948
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3949..>4914)
                     /locus_tag="PK977_03270"
     CDS             complement(<3949..>4914)
                     /locus_tag="PK977_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF17155.1"
                     /translation="RQLSGGVTTSHILHGSANVIGGQTQLIKLRWGANDDDLKFKGWP
                     GQIKFALGENVKRSSSAISNASNNNRYPDTRMGVEQVLIDAFTRAKDYQKAWKEYNDA
                     KEKLIKEKKSTASLVAPRRDLELDALVEILEKKRFITCHSYVQSEINGAMKVAEQMGY
                     TVNTFTHILEGYKVADKMKKHGSFASTFSDWWAYKMEVEDAIPYNAAIMQKVGLTVAI
                     NSDDAEMARRLNQEAAKIVKYGGVTEEEALKMVTLNPAIMLHVDDKVGSLKVSKDGDV
                     VLWSDHPLSIYAKSLYTIVDGTVYFDRQKDEAMQKQVDDERLRLVR"
     assembly_gap    4915..5010
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5011..6996)
                     /locus_tag="PK977_03275"
     CDS             complement(<5011..6996)
                     /locus_tag="PK977_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF17156.1"
                     /translation="MRKPNTWLAMVCSIFFSLPLWAQPTFPENGVADPRHGHYAFTNA
                     TIVKDAVTTLTNATLVIKDGKIVAVGSNLKVPAGAVEVDCKGKFLYPSFIDIYADYGT
                     PAAQRTTGGGFGGFTPGQQAQLSTNTKGAYGWNQAIKSDVDAYKVFVVDDAKAKPLRD
                     AGFGTVLAHVKDGIARGTGTVVTLANEKENLVMLKERASAHYSFSKGTSTQSYPGSMM
                     GMIALLRQTYLDAQWYKQRPAAEGINLSLQSWNDNQGLPQIFEANDKWNNLRADRIGD
                     EFGVQYIIKGGQNEYQRIKEMKTTNATFILPLNFPQAQDVEDPNEARFVALTDMKHWE
                     LAPTNVAAFEKAGIPFCLTTADLRDVRSFWSSLRKAMEYGLSETKAMEALTKTPATLL
                     GVYDKVGSLDAGKLANFVITNGPIFNEKTAILFNYVQGIKYTVKEENLIPGTYNLAIN
                     TPSGTESYTLDVKSSSSATMFAKDTLNAKFTQDGKMIKLNYAPLTRRMRPQGATDGQR
                     PPMGSGGFGRGGTTDQTLPANAARLSGVNNGKEWNGTGIDSLGNSLTWTATLVKETEM
                     KTDSAKKKDMPAIGKVVYPFMPFGWEDGQQPKQENILIKNATVWTNEKEGVLQNADVL
                     IKNGKIAAVGKNLSDASARVIDGTGKHVTPGIIDEHSH"
     gene            7126..8067
                     /gene="deoC"
                     /locus_tag="PK977_03280"
     CDS             7126..8067
                     /gene="deoC"
                     /locus_tag="PK977_03280"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRF17157.1"
                     /translation="MKTNHFTMAVAKLISVLPDFSLSPRIDQVGVEERASRFTKRSIK
                     KESKMNGLKLVLNMIDLTTLEGKDTEGKVKQLCYKAMHPHDAVPGIPTVAAVCVYPSM
                     VRVAKKALDGSGIKVASVATAFPSGQAPRDVKIRDTKYAVNEGADEVDMVISRGKFHS
                     GEYNFVFDEIAAIKEACGDARLKVILETGELGTFDKVRRASDLAMHAGADFIKTSTGK
                     IQPAATMPVTLVMLEAIRDFYYKTGKMIGMKPAGGISKSKLALHYLVMVKEVLGENWL
                     NNEWFRFGASSLANDVLMQIVKEQTGLYQSANYFSVD"
     gene            8398..9900
                     /locus_tag="PK977_03285"
     CDS             8398..9900
                     /locus_tag="PK977_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF17158.1"
                     /translation="MAVTKKKAAAKKSNTIKLKFDTSWSYDPAPESKSAATISKQYDL
                     FINGEWTKPQSGKYFATINPANEEKLSEVAEANEADVNKAVASARNAYEKVWKKMPAK
                     ERAKYIFRIARMVQEKARELAIIESLDGGKPIRESRDFDVPTAANHFFYYAGWADKLE
                     YAFPNRRTEPLGVAGQIIPWNFPLLMAAWKIAPAIATGNTVVLKPAETTPLTALKLAE
                     IIQEADLPPGVVNIITGAGATGAAIVNHPDVNKIAFTGSTDVGKIIQRAIAGTNKKVT
                     LELGGKAANIIFEDAPIDQAVEGVINGIFFNQGHVCCAGSRLYVQESVAKEVIRKLKD
                     RLESLIVGDPLDKNTDIGAINSKQQLETIHKYIKIGRQEGAEMYESNCKLPGKGFFCR
                     PTLFTNVAQSNRIAQEEIFGPVLAILTFRTDDEVIEKANNSPYGLSAGVWTDKGSKIF
                     NMTKRMRAGVVWANTFNKFDPTSPFGGYKESGFGREGGLHGLLPYLNLVG"
     gene            9902..10363
                     /locus_tag="PK977_03290"
     CDS             9902..10363
                     /locus_tag="PK977_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17159.1"
                     /translation="MMFKAGHIEISTLEENGNVMLYWGERKLFFGKQDEATVQSLLLG
                     NFKLVRNHYDQKAKDKLTGEELDELSLTIVLHYLYMYNMWRGLYKGQENKQLEFDEED
                     FHHPQTHDEIIWFVKRKYPGDHIHRCSALMEMSESDFRDYEKRRQEFHDMF"
     gene            10386..11339
                     /locus_tag="PK977_03295"
     CDS             10386..11339
                     /locus_tag="PK977_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF17160.1"
                     /translation="MGKALIKKSSLNVSENGLERTTSAKRLDVLKTYKIYIGGQFPRT
                     ESGRYYIPTNAAGKKLANVCLSSRKDFRDAVVAARTVFGGWSGRAAFNRSQILYRMAE
                     MLEGRKAQFIYELVQQDSTKAKAEKEVNLAIDRLIYYAGWCDKFQQLFSAVNPVASSH
                     FNFSVPEPTGVVAIIAPQSDSLLGLVSVVAPVIAGGNTCVVLAAETKPLCAVTFAEVL
                     NSSDLPGGVVNILTGKPSELASWFVDHMDVNATIYCENNSDTQKTMREKSATNLKRIF
                     FYNNVNWYSEEGQSPYFIMDTQEIKTTWHPVENIAGAGGGY"
     assembly_gap    11480..11518
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11519..11846
                     /locus_tag="PK977_03300"
     CDS             <11519..11846
                     /locus_tag="PK977_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF17161.1"
                     /translation="KKQATINEVTSRDGRRTDKGFRLMIISTNNRDEAIAAKTKVYTY
                     FPELKAYLWYQSPYFRVKAGNFKDRKDAEAYQKRLNVYFPKGVFIMKDIIEVKPGRGE
                     EDDIQP"
     gene            11939..13117
                     /locus_tag="PK977_03305"
     CDS             11939..13117
                     /locus_tag="PK977_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRF17162.1"
                     /translation="MQSRIKKLAQQYASEFIDIRRHLHAHPELSYQEFETSKFVQGKL
                     TEFGIPFEVKATTGVVGLIKGKNPGSRIIALRADMDALPIKEENDIPYKSKNEGVMHA
                     CGHDVHTACLLGAAKILNELKEEWEGTVKLIFQPGEEKNPGGASLMIKDGVLENPKPQ
                     AILALHVNTILEVGQLSFRGGKVMASADEIYITVKGKGGHAASPHLTIDPILISSHLV
                     IALQQLVSRNNNPFNPSVLSITSFNGGNTTNVIPNEVKLMGTFRAMDEEWRFKAHELI
                     RKITTDLVSSMGGTVDLHIDVGYPCVMNNEKVNASARKKAEEYMGAKYVSETELRMGA
                     EDFGYYAQQIPACFYRLGTMNTAKGITAGVHTPVFNIDESAIEIGMGMMAWLGSSLEV
                     "
     gene            13230..15572
                     /locus_tag="PK977_03310"
     CDS             13230..15572
                     /locus_tag="PK977_03310"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRF17163.1"
                     /translation="MHNELRKQQALEYHAKGRPGKIEVIPTKEAKTQRDLSLAYSPGV
                     AEPCLEIAENIENVYKYTAKGNLVAVISNGTAVLGLGDIGPEAGKPGMEGKGVLFKIF
                     ADIDVFDIEINEKDPEKFVQIVKALEPTFGGINLEDIKAPECFYIEKKLKEKLHIPIM
                     HDDQHGTAIISAAALLNAAELQKKKLDKMKFVVNGAGAAAISCMKLYIALGAKRENFR
                     VFDSKGLIHESRTDLDELKKEFIAAGKNVSLAEAMKDADVFIGLSKGGVLNGEMVNSM
                     AKHPIVFAMANPDPEISWEEATEARKDIIMATGRSDYPNQVNNVLGFPYIFRGALDVR
                     AEQINEAMKLAAVKALAELTKTPVPDIVNMAYNETNITFGPIYIIPKPLDPRLLATVA
                     PAVAKAAMESGVAKQPIHHWDNYVNQLNKRLGLDNQVMRAIGNKARRDPKRIVFAEAD
                     NVKILKAAQIVYDEGVGYPILLGDVNKIKTMAEANSIDLSDLPIFDPRSDEMEEKRNK
                     YSQIFFAKRGRKGFNDYESKKIMKDRNYFGCMMVECGDADAMISGLAKNYPDTIRPAL
                     QTIGTEEGVKKIAGMYLMLTKKGPLFLADTTVNFNPTAEELAEITLMVAREVKHFNIT
                     PRIAMLSYSNFGSSNSPEAKLVAHAREIVKQKMPSLIVDGEMQASVAFSNELIKENYP
                     FSELVDQEVNTLIFPNLASGNIAYNLMKELDIADAVGPVLLGLKKPVHVLQLGSSVRS
                     IFNMALIAVVDAQIKSKTSTQEIVQRSPWWKRFRKVSKTI"
     gene            15656..>16169
                     /locus_tag="PK977_03315"
     CDS             15656..>16169
                     /locus_tag="PK977_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF17164.1"
                     /translation="MTIFSDIGRYLLMIKGMFSKPENHKMYWKEFVHQCTEIGFGSLG
                     IVAIISIFIGAVSTIQTAYQLVSPLIPQSTIAQIVRDTVILEFAPTLVCIVLAGVAGS
                     RIASELGNMRVSEQIDALEIMGINTKAYLILPKIVAALLMIPLLVVIAMALGIWGGRL
                     AGVASGIISGS"
     assembly_gap    16170..16179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16180..16401
                     /locus_tag="PK977_03320"
     CDS             <16180..16401
                     /locus_tag="PK977_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF17165.1"
                     /translation="YDKGLLEFFVPYNVIFALIKAYVFSFIISSIPAFYGYYVKGGAL
                     EIGRASTKAVVVSCILILFADYILSALLL"
     gene            16404..>17256
                     /locus_tag="PK977_03325"
     CDS             16404..>17256
                     /locus_tag="PK977_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRF17166.1"
                     /translation="MRFYSRKNYIAISLLLLIAAINIFFFFKRNKFGTYKHYVSYDNL
                     YSEKNNQPELRKRWNNTFRFNGIQEKDSARKLAISKAKITSNDSSLQEIIKIAKWISN
                     EFRGISKGQPIDSVLRLPMLQQYEATVKNQTPIWCGTYANMFLFFCKASNIETRIIEF
                     FKPGDHHVINECYLPESKKWIAVDLTYNYIFFQDENYKLLNTLDVISAVDNNRSIYVN
                     QVVSDSLVIKKISGNQIIWEKYLQFNPDIYYYKETNPAHIYTTKEKIKRYIFPVSHYS
                     IFTTAKQS"
     assembly_gap    17257..17294
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17370..>17569
                     /locus_tag="PK977_03330"
     CDS             17370..>17569
                     /locus_tag="PK977_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF17167.1"
                     /translation="MIEVKNIQKSFGDKTVIHDVSAVMKAGQCNLIIGSSGSGKTVLM
                     KCMVGLFEPDKGEVLYSGQNFTA"
     assembly_gap    17570..17684
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17685..18189
                     /locus_tag="PK977_03335"
     CDS             <17685..18189
                     /locus_tag="PK977_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF17168.1"
                     /translation="FTDWSHKKKLDRVNEVLARVNIADANRKFPAELSGGMKKRVALA
                     RAIVLNPKYLFCDEPNSGLDPQTSLVIDKLIREITVEYNITTVVNTHDMNSVMEIGNY
                     ILYMYQGHKEWEGNNKEIIFSKNQRLNDFIFASEFLQDAKDMRVLEETGKIPHHRNFD
                     NKGDLLK"
     gene            18254..>18730
                     /locus_tag="PK977_03340"
     CDS             18254..>18730
                     /locus_tag="PK977_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRF17169.1"
                     /translation="MSILVNKNSKILVQGFTGTEGTFHASQMIEYGTNVVGGVTPGKG
                     GSKHLEKPVFNTVSEGVKATGADVSIIFVPPAFAADAVMEAAEAGIGLVVCITEGIPV
                     QDMVKVKNYLQGKNTRLVGPNCPGVITAGECKVGIMPGFVFIPGRIGIVSKSGTLTY"
BASE COUNT         5344 a         3741 c         3934 g         4983 t
ORIGIN      
        1 aatgcagtct atgagaagcg taatcatgcc ctgtttggag aaagattggc cttggatatt
       61 gacacttcat tttcaatggt tgcagaaggt cttgttagct ccttccgtga acaggaagat
      121 tatgaagggt tcaaaatggc ctgtatcgtc aactttggat tagacacaac aattgaagaa
      181 gaagaattca aaaaaggtga cctgaataca ttgactgacc gtatttattc tgaagccact
      241 gaaaaataca accggcacaa agaagaatta cagaaacagg caataccggt cttcaaaaac
      301 atccgtctga cacaaggaag ccacatagaa aatgtaatag ttccttttac agatggacgc
      361 aagggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaagaca
      421 ttgcagtcaa atgcagttga attgacgaat gcactggaaa agaccatcac tctggcggta
      481 atcgatgatg catggaaaga gcacctgcgg gctatggatg atttgaaaca aagtgtgcag
      541 acagcctacc ttgaacaaaa agacccgctg gttatatata aagtggaggc ttataaccag
      601 ttccgcaaca tgaatgcaga agtaaataag gatatnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg actatgcagc
      781 caatgagaag gataaactgg taccagtaac cgtcgcaccc aaaataggac gcaacgatcc
      841 atgtccttgt gggtcaggta agaagtacaa gcattgccat ggtaaagatg cctgatgggg
      901 tatataatac tgacgagaaa ggagcattta gatgctcctt tctcgtttta tggaatgtgc
      961 aaaaacagct tattcccggc ttgtagtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaacca
     1141 actacaacag aacccaccac tacacccaca actacagaac cagcacctac cagcaccaat
     1201 gctgatggtg ttccttcatt ctctgatcct gaagtacaga aattcgccaa tgattatgca
     1261 gcctttgtaa aggaatacaa agaaggtatg aaagacccgg caaaactttc tgctttagca
     1321 cagaaaatgc aagactggtc aaaaagggca acagaaatag gtaccaaact ggccacatct
     1381 cctgatgaag ctaaaaagtg gactgactgg actatggcaa ttgctaaaga actgatgccg
     1441 caataagata tttgcagctg aatgcaataa taaatccccc cggtaacatt atcgggggga
     1501 ttttatttta actttagtta ccaaatctaa tctgcccgta tgtataaggt gaccaaaaaa
     1561 aaggtgcatg gcctgttgca tcctgaaatt gtcggagata aacattggga taagatcatc
     1621 aatattttta tcatttcact gatcattctg aatgtgatcg ctgtaatgct ggaaaccgtt
     1681 tcatcattgc atgaaaaata tgcggacttc tttcactact tcgatgttgt ctctgttatc
     1741 atctttagta tcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atcatgaaga
     1801 aaaatacaag ggaagcctta agggaaggtt gagatacatg ctatcccccg gcgctttggt
     1861 tgacctcttg gccatacttc ctttttacct gcatgccatc attggtttcg acctgcgaat
     1921 gctcagaatt ttacgtctta tgcggttttt ccgtttgttc cggcttaccg cctacatgaa
     1981 atctgcgcat atgatctcga atgtattcaa aaaaaggatc aacgaattgg cacttagcct
     2041 ggtgctggta atttttctta tcatcattgc atccagtgcc gtttattttg ccgaacacca
     2101 gcatatagaa aacagagccn nnnnnnnnnn nnnnccaaat tcaccagtat accggctacc
     2161 atctggtggg ccgtggtaac gcttaccacc accggctatg gggatatgta tcccatgact
     2221 tatttaggta aagctatggc catggtgatt atgttgacag gtgttgcctt ctttgcttta
     2281 cctgccggta tcatcacagc tggcttcctg gaagagttca ggatgaacag aatcaaaaag
     2341 acacatcggt gccccaattg cggtgaacaa ttagaattag acgacaataa ccatgctcat
     2401 cgaactgacc cataaaaaag gccggtcata aaccggcctt ttttagtgat aattcttctt
     2461 atacattaga aacctttctt tccttttatc tcatacttct gctcaaaccg ctcattcagc
     2521 aatttgtgtt tgtcagtcag gtctattttg cggccttgta taaatgcatc tgttacattg
     2581 ctggtacgca tatcaaggat atctccatca cttaccacta tattggcatc tttgcctgct
     2641 tcaatacttc ctgttttgtc tgctactccc ataatctttg ccgcatttag agtaattgca
     2701 gccagagctt cttcttttgt caatccataa gcagccgctg taccggcatt aaaaggcagg
     2761 ttgcggccac gggtctgact gtcatcatca gagatagaaa annnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnncgccgt tttatagggc tggtccacat cgtcatcagt agccgtaggc
     2881 aaactatgcg gttgctgtaa aataacagat acattatgct tctttaaaag atcagccacc
     2941 tggtagctat cgcttccgcc aactatgacc atatcaacgc caaactcttt tacaaaatca
     3001 agggccacaa gaatctgttt gacagtgttc gcattaacat acagcttttg cttcttttca
     3061 aacaaacctt tcaccgcttc aaacttcagg ttggtttctt catgtttacc ttccgcctgg
     3121 taggctttgg cttcacggaa aaacaccttc actttttcaa tttgttccag cccttcttta
     3181 accggatcag tttgtgaggc cgacggacct gcaccaaagc caccacctct gaaaccaccg
     3241 gatctggcca tcagcgaagg caggttaaga tgtatccctg catctgtttt ataagctgca
     3301 tcttcccagt tccatgcatc cagttgtact actgaagatg agccagtcag taaactgcct
     3361 tgcggtatca cattcgccaa taatatcccg ttggaacgga gtgtattgat cactcttgaa
     3421 tcagtgttat aagccacaat agctcttaca ttgggattta aatctccaat ttccctaaca
     3481 tcagtagtgg ccctgttggc ggatatctcc accaaaccaa gactactggt tggcaagatc
     3541 aatcccgggt atacatgttt cccttttaca tcataaacac taacatcatc tgcaggaaca
     3601 ggcaggctgg cacccacttc ctctattttg ccatccttta tttttatagt accattttca
     3661 atcactttgc cattccctac atggatggta gcatttttta tataaataat gcccttttgt
     3721 ggtttggcag gcaatactgt ttcctgggca gttgccgcaa gggtgaaaca ggttgtcatt
     3781 ataagaaata tctttctcat tactgttggt ttagtcgtta ttaatatcat cagaatctat
     3841 caccagcaaa ccatggctat gaccatgatc gctgcaggta tgcatgatct ggtaagttgg
     3901 ctgcgccgga atcacgggtg caccgcttct nnnnnnnnnn nnnnnnnncg taccagcctt
     3961 agcctttcat catcgacttg cttttgcata gcctcatcct tctgcctgtc gaaataaacc
     4021 gtgccatcta caatggtgta aagtgattta gcatatatgc tcagcggatg atcgctccac
     4081 agcaccacat caccatcttt actcactttc aggctgccta ccttgtcatc cacatgcagc
     4141 atgatagcag ggttcagcgt aaccattttt aatgcctctt cttctgtcac accaccatat
     4201 tttacaatct tggctgcctc ctgattaagc ctgcgggcca tctctgcatc atcggagtta
     4261 atcgcaactg tcaacccaac tttttgcatg atggccgcat tataaggaat ggcatcttca
     4321 acctccattt tgtatgccca ccagtcggag aaagtagaag caaaagagcc atgctttttc
     4381 atcttatccg ccaccttata tccttccagg atatgcgtaa acgtattcac ggtatatccc
     4441 atctgctcag ccaccttcat agcgccatta atttcgcttt gcacataaga gtgacaagtg
     4501 atgaaacgct ttttttccaa tatttccacc agtgcatcca gttcaaggtc acggcgggga
     4561 gcaactaatg atgctgttga ttttttttct ttgataagct tttcttttgc atcattatat
     4621 tccttccatg ccttttgata atcttttgcc ctcgtaaagg catcaatcag cacctgttca
     4681 acacccattc tagtatcggg atagcggtta ttgtttgaag cattagaaat agcggaagaa
     4741 gaccgtttca cattctcacc taacgcaaat tttatctggc cgggccatcc tttaaacttc
     4801 aggtcgtcat cattggctcc ccagcgaagt tttatcagtt gcgtttgtcc gccaatcaca
     4861 ttggccgagc catgcaggat atgtgatgtg gtaacaccac cactcaactg gcggnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgtgagtgc tcgtcaatga taccgggagt
     5041 tacatgcttt ccggtaccat ctataactct tgcagaagca tcagacaggt ttttgcctac
     5101 tgctgcaatc tttccgtttt tgattaatac atcagcattt tgtaacacac cttctttctc
     5161 gttggtccat accgttgcgt ttttgatcaa aatgttttcc tgctttggct gctgaccatc
     5221 ctcccagcca aaaggcataa aagggtaaac cactttacca atggcaggca tgtctttttt
     5281 cttcgcagaa tctgttttca tctctgtttc cttcaccagg gtagctgtcc atgtcaatga
     5341 attacccagt gagtcaattc ccgttccatt ccattcttta ccattattta caccacttag
     5401 ccttgcagca ttggcaggta atgtttgatc ggttgttcct ccccgtccaa aaccaccgga
     5461 gcccatcgga ggtctttgcc catctgttgc cccctgcgga cgcatacgcc ttgtcagcgg
     5521 ggcataattc aattttatca tcttgccatc ctgggtaaac ttagcattca gggtgtcttt
     5581 tgcaaacatg gtagcagaac tgctgctctt tacatccaat gtatagcttt cagttcctga
     5641 tggagtattg atggcaaggt tgtaggttcc tggaattaaa ttttcttctt ttacggtgta
     5701 tttaattcct tgaacataat taaaaagtat agctgttttt tcattgaata taggcccgtt
     5761 ggtaatgaca aaattggcca gcttacctgc atccaggctg ccgactttat cataaacacc
     5821 taataaggtt gccggagttt tagtgagtgc ctccatcgcc tttgtctcgc tgagtccgta
     5881 ctccattgct ttgcgaaggc tggaccagaa tgatctcaca tctcttaaat ctgctgttgt
     5941 taaacagaag ggaattcctg ccttttcaaa agccgctaca ttagtggggg ccagttccca
     6001 gtgtttcata tcagtaagtg ccacaaacct tgcttcattg ggatcttcta catcctgtgc
     6061 ctgcggaaaa ttcaatggca ggatgaaggt ggcattggta gtcttcatct cttttattcg
     6121 ctggtattca ttttggccac ctttaatgat atactgtaca ccaaattcat caccgattct
     6181 gtcggcacgg aggttattcc atttatcatt ggcttcgaat atttgaggca gtccctggtt
     6241 atcattccat gactgtaaac ttagattgat tccttccgca gccggtcttt gtttatacca
     6301 ctgggcatca aggtatgtct ggcgaagtaa tgcgatcatt cccatcatag agccaggata
     6361 actttgtgta gaagtcccct tactgaaaga ataatgagcg gaagctctct ctttcaacat
     6421 taccaggttt tctttttcat tggccagtgt aaccactgta ccagtaccac gggcaattcc
     6481 atcttttaca tgagccagca ctgttccaaa acctgcatct cttaatggtt tggccttggc
     6541 atcatccaca acaaatactt tgtaggcatc cacatcgctt ttaatggcct ggttccagcc
     6601 ataagctcct tttgtgttgg tgcttagttg tgcttgctga cctggggtaa atccgccaaa
     6661 gccgccaccc gtagtacgct gggcagcagg tgtgccatag tccgcataaa tgtcaataaa
     6721 ggatgggtaa aggaatttgc ctttacaatc cacttctaca gctcctgccg gaactttcag
     6781 gttgctgcca accgccacta ttttgccatc cttaatgaca agtgtggcat tggtcaaagt
     6841 ggtaacggcg tcttttacaa ttgttgcatt cgtaaatgca tagtggccat gccgcgggtc
     6901 ggctacgcca ttttcgggga aagtaggttg ggcccagagt ggtaaactga agaaaatgct
     6961 acataccatc gccaaccagg tgttgggttt tctcattgca atgtttgttt ttaaaggaga
     7021 ccgaatttaa cgcaatccgg tcgttgataa taataaataa caggacgaaa ggttggattt
     7081 gacgtaaatc cggcaactat cctttcctga aaactttggt tatctttgaa aactaatcat
     7141 ttcactatgg cagtggccaa attaataagt gttttgcctg actttagtct gtctccccgc
     7201 attgaccagg tgggggtaga agaaagggcc tcccggttta ccaaaagaag cattaagaaa
     7261 gagtccaaaa tgaacggcct caaactggtg ctgaacatga tcgaccttac cacccttgaa
     7321 ggaaaggata cagagggtaa agtaaaacag ctatgctaca aggccatgca tccccatgat
     7381 gccgtacctg gcatccccac agttgccgcc gtttgtgtgt atcccagtat ggtcagagtg
     7441 gcaaaaaaag cactagatgg ttcgggcata aaagtagctt cggtagcaac tgcttttccc
     7501 agcgggcagg cgccaagaga tgtaaagatc agggatacta aatatgcggt gaatgaagga
     7561 gccgatgagg tggatatggt gatcagccgc ggtaagtttc attctggtga atacaatttt
     7621 gtgtttgatg aaatagccgc catcaaagaa gcctgtggtg atgcgagact gaaagtgata
     7681 ttggaaacag gtgagctggg cacttttgat aaagttcgtc gggccagcga tcttgccatg
     7741 catgcgggag ccgattttat caagacctct accggaaaga ttcagccggc cgctaccatg
     7801 ccggtaacgc tggtgatgct agaagcaatc cgtgactttt attataaaac cggcaaaatg
     7861 ataggaatga aaccggcagg tggcatttcc aaatcaaaac tggctttgca ctacctggta
     7921 atggtgaaag aagtgttggg cgagaattgg ctgaacaatg aatggttccg ctttggcgcc
     7981 agcagtttgg ccaatgatgt gttgatgcag atagtgaagg aacaaaccgg gctttatcag
     8041 agtgccaatt atttttcagt ggattaagaa gtatcaacca ttattgttga aaaaattttg
     8101 gaaaacatac agattatgga ttgaaataac tgtgctcact gcatgtttaa tccttatgat
     8161 atattataaa ttttgggagg aagtaccaga aaaaagatgg ttgagatgga ctcagttttt
     8221 tttgttctta gcttattgga ttgaccggtt tgtaaaattg ataaacaata aaaaagtcac
     8281 aaataccaga taattaagct ccgtggtaat ttttgatgat aagaaatcag aatgactaag
     8341 agtgagaagc aacagaagac catctgtgtt actatcgctg gataaataaa aaatattatg
     8401 gccgttacaa agaaaaaagc tgcagcaaaa aaaagcaata cgatcaaatt aaaatttgat
     8461 accagctggt cctacgaccc tgcaccggaa agtaaaagtg ctgccaccat cagcaaacaa
     8521 tacgaccttt tcattaatgg cgaatggaca aaaccgcaaa gcggtaaata ttttgccacc
     8581 atcaacccgg ccaatgaaga aaaactgagt gaagtagcgg aggccaatga agccgatgta
     8641 aacaaggctg tggcgtctgc cagaaatgcc tacgaaaaag tgtggaagaa aatgccggcc
     8701 aaagaaaggg ctaaatacat tttccgaatt gcccgcatgg tgcaagagaa ggcaagggaa
     8761 ctggccatca tcgaatcatt ggatggcggt aaacccatcc gggagagccg cgactttgat
     8821 gtgcccactg ccgccaatca tttcttttac tatgcaggct gggccgataa actggaatat
     8881 gctttcccca acaggagaac ggaaccattg ggtgtggcag gccagattat cccctggaat
     8941 tttcctttgt taatggctgc ctggaaaatt gcaccggcaa tagccaccgg taatacagtt
     9001 gtattgaaac cggctgagac cactccactt actgctttga aattagcgga gataatacag
     9061 gaagcagatc tgcctcccgg cgttgtgaat attattaccg gagcaggtgc caccggtgct
     9121 gctatcgtta accatcctga cgtaaataaa attgcgttca ctggttctac agacgttgga
     9181 aaaattattc aaagggccat tgccggaacc aataaaaaag taacgctgga actgggcggc
     9241 aaagcagcca atatcatttt tgaagatgca cccattgacc aggccgtgga aggggttatc
     9301 aatggtattt tctttaacca gggtcatgtg tgctgcgccg gatcaaggtt gtatgtacag
     9361 gaatcagtag cgaaagaagt gatcagaaaa ttaaaagacc gcctggaatc gctgatagta
     9421 ggcgacccgc tggataagaa tacagatatc ggcgctatca attccaaaca acaattagaa
     9481 accattcaca aatacattaa gataggcaga caggaaggcg ccgaaatgta tgagagcaat
     9541 tgcaaactcc ctggcaaagg atttttctgc cgccctacct tgtttaccaa tgtggcgcaa
     9601 agcaaccgta tagcacagga agaaatattc gggccggtat tagccatcct gactttcaga
     9661 acagatgatg aagtgattga gaaagcgaac aactcgccgt atggtttgag tgccggtgtg
     9721 tggaccgata aaggttccaa gatttttaat atgaccaaac gtatgcgggc aggtgtggtt
     9781 tgggccaata cgtttaacaa gtttgatcct acatcgcctt tcggcggcta taaggaaagt
     9841 ggttttggaa gagagggagg actacatgga ttgttgccct acctcaactt agtggggtaa
     9901 tatgatgttt aaggctggcc atattgaaat aagcactttg gaggaaaacg ggaatgtgat
     9961 gttgtattgg ggagaaagga aattgttctt tggcaaacag gatgaagcaa ccgttcagtc
    10021 tttgctgctg ggtaatttta agctggtaag aaaccattat gatcaaaaag caaaagacaa
    10081 actgaccggt gaagagttag atgagttgag cctgactatt gttttgcatt atctctatat
    10141 gtataatatg tggagaggcc tttacaaagg acaggagaac aagcaactgg aatttgacga
    10201 agaagacttt catcatccgc aaacccatga cgagatcatt tggtttgtaa aaagaaaata
    10261 tcccggtgat catattcacc gctgcagtgc attgatggaa atgagtgaga gtgatttccg
    10321 ggactatgaa aaaagaagac aggaatttca cgacatgttt taactgaaaa tattaagctt
    10381 tacaaatggg aaaagcactt atcaaaaaat catcacttaa cgtaagtgag aatgggttgg
    10441 aaagaaccac ctccgctaaa agactggatg tgctgaaaac ctacaagatc tacattggcg
    10501 ggcaattccc cagaacggaa tcaggccgtt attatatacc caccaatgca gccggaaaaa
    10561 aactggccaa tgtatgccta agctcccgca aagatttccg ggatgccgta gtagctgcaa
    10621 gaactgtctt tggcggatgg agcggccgtg ccgcttttaa ccgcagccag atactctacc
    10681 ggatggcaga aatgctggaa ggcagaaaag cacaattcat ttacgagctg gtgcaacagg
    10741 attcaaccaa agcaaaagca gaaaaagaag tgaaccttgc catcgacagg ttaatctatt
    10801 atgccggctg gtgcgacaag tttcagcaac tgttcagcgc tgtaaacccg gtggccagtt
    10861 cgcattttaa tttttctgtc ccagaaccta caggtgtggt agccattatt gctccgcaaa
    10921 gtgattcatt gttgggatta gtttctgttg tagctcctgt tatagccggg ggtaatactt
    10981 gtgtagtgct tgccgctgaa accaaaccac tttgtgctgt aacttttgca gaagtgttga
    11041 actcatctga cctgcccggt ggtgttgtta atattttaac aggcaaacca tcggaactgg
    11101 ctagctggtt tgtggaccat atggatgtga atgcaaccat ttattgtgaa aataattctg
    11161 atactcagaa aacgatgcgg gaaaaatcgg ctactaacct gaaacgtatc tttttctata
    11221 ataatgtgaa ctggtacagt gaagaaggac aatcgcccta cttcatcatg gatacccagg
    11281 aaataaaaac cacctggcac ccggtagaaa atattgccgg agcaggagga ggttattaaa
    11341 tttggccgga attttgaccc ttatgcctaa tatgaaattt ctttttgttc tcctgttctc
    11401 tttagtagca ggttcaggat ttgcacagac ggatagcact gcaccacaac cggttgacag
    11461 caattctgtt attgtacatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
    11521 aaaaacaggc aaccatcaac gaagtaacca gccgtgatgg ccgtcgtacc gataagggtt
    11581 ttcgcctcat gatcatcagc accaataaca gggatgaagc gattgctgca aaaacaaaag
    11641 tatataccta ttttcctgaa ctaaaagcct atctctggta ccagtctccc tacttccggg
    11701 taaaagccgg caatttcaaa gacaggaaag atgcagaggc ctaccagaag cgattgaatg
    11761 tttactttcc aaagggtgtt ttcatcatga aggatattat tgaagtgaaa ccgggaagag
    11821 gcgaagaaga tgatattcaa ccataaaagc acttagaaca ttgttaagcc atgccagaca
    11881 gcatggcttt tgttttatat ggttaattgt ctttcccctg aaatgttatt tttgccaaat
    11941 gcagtccaga ataaagaaac tagcccaaca atacgcatca gagtttattg atatacgccg
    12001 gcacttacat gcccaccctg aactgagcta ccaggaattt gaaacctcaa aatttgtaca
    12061 gggaaagctg acagaattcg ggattccttt tgaagtaaaa gccaccaccg gtgtggtggg
    12121 actgatcaaa ggtaaaaatc caggtagccg catcattgcc ctccgggctg atatggatgc
    12181 actgcccatc aaagaagaaa atgatatccc ctataaatcg aaaaatgaag gggtgatgca
    12241 tgcctgcggc catgatgtgc atacggcctg cctgttaggt gccgctaaaa ttctcaatga
    12301 attaaaagag gaatgggaag gaacagtgaa actgatcttt cagcccggtg aagaaaaaaa
    12361 ccccggtggc gcttccctga tgatcaaaga cggagtgctg gaaaacccaa aaccacaggc
    12421 tattttggca ttgcatgtca atactatcct ggaggtgggc cagcttagtt ttcgtggcgg
    12481 caaagtaatg gccagtgccg atgaaatata tataacagtg aaaggcaagg gcggtcatgc
    12541 cgcttctcct catcttacaa ttgatcctat attgatcagc tctcaccttg ttattgcttt
    12601 acaacaactg gtaagcagaa ataataatcc gttcaatccg tcagtactct caataacttc
    12661 ttttaatgga ggcaatacca ccaacgttat tcccaacgaa gtaaagttga tgggcacctt
    12721 ccgtgccatg gatgaagaat ggcgtttcaa agctcatgaa ttgatccgaa aaataacaac
    12781 tgaccttgtg tcctccatgg gtggcacggt tgacctgcac attgatgtgg gctatccctg
    12841 tgtaatgaat aacgaaaaag tgaatgccag tgcccgtaaa aaagcagagg agtacatggg
    12901 cgcaaaatat gtaagtgaaa cagaactgcg aatgggagca gaagactttg gttattatgc
    12961 gcagcaaata ccggcttgtt tttatcgcct cggcactatg aatacagcca aaggaattac
    13021 agccggcgtt catacccctg tttttaatat tgatgaatct gccattgaaa ttggcatggg
    13081 catgatggcc tggctaggaa gcagccttga ggtataatca ttacactaaa acgcctattc
    13141 tttcccgctg aaacctgcgg tcagatattt tgtaccttgc agtcccgttt agatagtcat
    13201 gttagaaaga tgaacattaa ataagtttta tgcataacga attgcgcaaa caacaggccc
    13261 tcgaatacca cgcaaaagga agacctggga aaattgaagt aatccctact aaagaagcca
    13321 aaacacaaag agatctctcc ctcgcttact ctcctggtgt tgccgaacct tgcctggaga
    13381 ttgcagaaaa tatcgaaaac gtatataaat acactgccaa aggaaacctc gttgccgtta
    13441 tcagtaacgg aacagccgtt cttggcttgg gcgatattgg accagaggcc ggcaaaccgg
    13501 ggatggaagg gaaaggggtc ttgtttaaaa tatttgctga tattgatgtt tttgatattg
    13561 aaatcaatga aaaagatcct gagaaattcg tacaaattgt aaaagcactg gagcccactt
    13621 ttggaggtat taatttggag gacatcaaag caccagaatg tttttacata gagaaaaagc
    13681 tgaaagagaa actacatatc ccaatcatgc atgatgatca acatggtact gctatcatca
    13741 gcgctgcagc attactcaat gcagctgaat tgcagaagaa gaaactggat aaaatgaagt
    13801 ttgtggtcaa cggtgcaggt gcagctgcta tatcttgtat gaaattgtat atagcattag
    13861 gtgccaaaag ggaaaacttc agggtgtttg atagcaaggg gctgatacat gaaagcagga
    13921 ctgacctcga tgagctgaaa aaagaattca tagcagctgg aaaaaacgtt tcgttggcag
    13981 aagccatgaa ggatgctgat gtttttatag gactcagcaa aggcggtgtg ttaaatggtg
    14041 aaatggtaaa tagcatggca aagcacccga ttgtttttgc catggcaaac cctgatcctg
    14101 aaataagttg ggaagaagcc actgaagcca ggaaagatat cattatggcc accggccgca
    14161 gtgattatcc caaccaggtg aataatgtat tgggcttccc ttacattttt cggggtgcat
    14221 tagacgtaag agcggagcag atcaatgaag caatgaaact ggcagcagtt aaagcccttg
    14281 ctgagttgac caaaacaccg gtgcctgata ttgtgaatat ggcttataat gaaaccaata
    14341 ttacgtttgg ccctatttat attattccaa aacctttgga tcctcgttta ttggccactg
    14401 tagcgccagc cgtagccaaa gctgctatgg aaagtggagt agccaagcag ccaattcacc
    14461 attgggataa ctatgtgaac cagttgaata aacgccttgg tttggacaac caggtaatga
    14521 gagcaatagg aaataaagcc cgacgagatc caaaacggat cgtatttgca gaagcagata
    14581 atgtaaagat ccttaaagct gcacagattg tttatgatga gggtgttggt tatcccattt
    14641 tattgggaga tgtaaataag atcaaaacaa tggcagaagc caattcaata gatctgtccg
    14701 atctgcccat atttgatccc cgcagcgatg agatggaaga aaaacgcaac aaatattcac
    14761 agatattttt tgccaaaaga ggaagaaaag gtttcaacga ttatgagtcg aagaagataa
    14821 tgaaagaccg gaactacttc ggttgtatga tggtggagtg cggtgatgca gatgccatga
    14881 tatccggatt agctaaaaat tacccggaca ccattcgtcc ggcactgcaa acaataggca
    14941 cagaagaagg cgtgaaaaaa atagccggca tgtacctgat gctgaccaaa aaagggcctt
    15001 tattcctggc tgacacaacc gttaatttca atcctactgc cgaggaactg gcagagatca
    15061 cactgatggt ggcccgggaa gtgaaacatt ttaatattac tccaagaatt gcaatgctaa
    15121 gctattccaa ttttggcagc agtaattcgc cagaagctaa gctagtggct catgcccggg
    15181 aaatagtaaa gcaaaaaatg ccttcattga tagtggatgg tgaaatgcag gcaagtgtgg
    15241 ccttcagtaa cgaacttatc aaggagaatt atccctttag cgaattagtg gatcaggaag
    15301 taaacacact gatattccct aacctggcat ccggcaatat tgcctacaac ctgatgaaag
    15361 aattggatat tgcagatgca gtaggtcctg ttttattggg tttgaaaaaa ccggttcatg
    15421 tacttcaatt aggaagttct gttcgcagta ttttcaatat ggcacttatt gctgtagtag
    15481 atgcacagat caagagtaaa acatctacac aggagatcgt tcagcgttcc ccctggtgga
    15541 aacggttccg gaaagtcagc aagactattt aactcagaaa aatccccgtc tgaaatgggg
    15601 attttttgta tggctgtcca ttttacattt agtattttac agccttaatt aacgcatgac
    15661 gatattcagt gatataggac gttacctgct gatgatcaaa ggcatgtttt ccaaaccgga
    15721 aaaccataag atgtattgga aggaatttgt tcatcaatgt acagaaatag gctttggctc
    15781 attgggcatc gtagctataa tttctatttt tattggagct gtatccacta ttcaaactgc
    15841 ctaccagtta gtcagcccat tgattcctca atccaccatt gcccagatag tgagggatac
    15901 ggttattttg gagttcgccc ctacccttgt ttgtattgta ctggcaggtg tggcaggaag
    15961 ccgtattgca agtgaattgg gaaatatgcg ggtaagtgaa caaatcgatg ccctggaaat
    16021 catgggtata aatacaaaag catacctgat cctgcctaag attgtagctg ctttgctgat
    16081 gataccctta ttggttgtta ttgcaatggc gcttggaatt tggggcggaa gattggccgg
    16141 tgtggcatcc ggtataattt ccggtagtgn nnnnnnnnnt acgacaaagg actactggag
    16201 ttctttgttc cttacaatgt aatctttgcg ctgatcaaag cctatgtttt ttcttttatc
    16261 atttccagca taccggcttt ttatggatac tatgtgaaag gtggtgcgtt ggagataggg
    16321 cgggccagca caaaagcagt ggtagttagc tgtatcctga ttttgtttgc tgattatata
    16381 ttatcagcct tattgctcta attatgcgat tttatagcag aaaaaattat atagcaatat
    16441 cattgcttct gcttattgca gcgattaata tttttttctt ttttaaaaga aacaaattcg
    16501 gaacatataa acattatgtt tcttacgata atctctactc cgaaaagaat aatcaacctg
    16561 agctaaggaa aagatggaat aacacttttc gctttaatgg cattcaagaa aaagattctg
    16621 cccgtaaact agcaataagt aaagcaaaaa ttacttctaa cgattcctct cttcaagaaa
    16681 taataaaaat tgcaaaatgg atttcaaacg agtttagagg tatcagtaaa ggtcagccta
    16741 ttgactctgt tttgcgctta ccaatgctcc aacagtatga ggctaccgtt aagaatcaaa
    16801 ccccaatatg gtgtggtacc tatgcaaaca tgtttctctt cttttgcaaa gcaagcaata
    16861 ttgaaacaag aataattgaa ttttttaaac ctggcgacca ccatgttata aatgaatgct
    16921 atttgccaga atcaaaaaaa tggatagcag ttgaccttac ttataattat atcttttttc
    16981 aggatgagaa ttataaactt ctaaacactc tggatgttat ttcagctgta gataacaaca
    17041 gatcaattta tgtaaatcag gttgtatcgg atagtcttgt aataaaaaaa atttctggga
    17101 atcaaattat ctgggaaaaa taccttcagt ttaatcctga tatctactat tataaggaaa
    17161 caaaccctgc tcatatttat actactaagg aaaaaattaa aaggtatatt ttcccagttt
    17221 cacattattc gatttttacg acagctaaac aaagcannnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnttttat ccgtactttt ttcatatata ctctcttttt attgattgtt
    17341 tatttacttt acagattatt taaggggaaa tgatcgaagt aaaaaacata cagaagagtt
    17401 ttggggataa aacggtcata cacgatgtaa gtgcagtgat gaaggcaggc cagtgcaacc
    17461 tgattatcgg aagcagtggc agtgggaaaa ctgtgctcat gaagtgtatg gtaggtttgt
    17521 ttgaaccgga taaaggtgag gtattatata gcggtcagaa tttcacagcn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtttac cgactggagt
    17701 cataaaaaga aactggatcg tgttaatgag gtgctggcaa gggtaaatat tgccgatgcc
    17761 aaccggaaat tccctgctga actaagtggt gggatgaaaa aaagggttgc cctcgccagg
    17821 gcaatagtat tgaaccccaa atatcttttt tgcgatgaac ctaattcagg actggatccg
    17881 caaacatcat tggtaattga caagcttatc cgtgaaatta ctgttgaata taatattacc
    17941 acagttgtca atactcacga catgaacagt gtgatggaaa taggaaatta tatcctttac
    18001 atgtaccagg gccacaaaga atgggaaggc aacaataaag agatcatttt cagcaaaaac
    18061 caacggctaa atgatttcat ttttgcctcc gaattccttc aggatgctaa agacatgagg
    18121 gttcttgaag aaaccgggaa aattccacat caccggaatt ttgataataa gggcgacctt
    18181 ttgaaatagc tttcttgtag cttctggaaa ggcccaaatc ccctaatttt gcggcaactt
    18241 tatatatctg catatgagca ttcttgtaaa taaaaactcc aaaatccttg ttcagggttt
    18301 tacgggcaca gaaggcactt ttcatgcctc tcaaatgatt gaatacggca ctaatgtggt
    18361 aggcggtgta actcccggaa aaggtggcag caagcatctt gaaaaaccag tatttaatac
    18421 ggtttcagaa ggtgtaaaag ctactggtgc cgatgtaagc atcatttttg ttccccctgc
    18481 ctttgcagct gatgctgtaa tggaagcagc tgaagcagga attggtttgg tggtatgtat
    18541 tactgaaggt attccggtac aggatatggt gaaagtaaag aattacctcc agggcaaaaa
    18601 taccaggctg gttgggccca attgccccgg tgttattacc gctggtgaat gtaaagtggg
    18661 tatcatgccc ggctttgtat ttattcccgg ccggatcggt atcgtttcaa aatcagggac
    18721 tcttacctat
//
LOCUS       DAORLS010000023        18679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1590_length_18679_cov_1.953232, whole genome shotgun sequence.
ACCESSION   DAORLS010000023 DAORLS010000000
VERSION     DAORLS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18679
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_18679_cov_1.953232"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1208)
                     /locus_tag="PK977_03345"
     CDS             complement(48..1208)
                     /locus_tag="PK977_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRF17170.1"
                     /translation="MADILIIDDEKAIRKTLTEILSFEGYKIEEAADGEEGLKKFKEK
                     NFDLVLCDIKMPKLDGIEFLQKAGEINADVPVIMISGHGNIETAVEAVKKGAYDYISK
                     PPDLNRLLITIRNAMDKSSLVTETKVLKRKVSRVQEMIGESVPIKKIKETIEKVAPTD
                     ARILITGENGVGKELVARWIHEKSNRASGPIIEVNCAAIPTELIESELFGHEKGSFTS
                     AIKQRIGKFESANGGTLFLDEIGDMSANAQAKVLRALQEGKITRVGADKDISVDVRVI
                     AATNKDLLKEVEAKNFRLDLYHRLSVILIHVPSLNDRRDDIPLLVDKFLQDICSEYGI
                     AKKPIAEEAIKLLHEYNWTGNIRELRNVVERLVILSGKTITAEDIRSYVMPK"
     gene            complement(1457..2446)
                     /locus_tag="PK977_03350"
     CDS             complement(1457..2446)
                     /locus_tag="PK977_03350"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRF17171.1"
                     /translation="MARLLTFREALREAMTEEMRRDDRVFLMGEEVAEYNGAYKVSQG
                     MLAEFGPRRVIDTPISELGFAAVGVGAAQNGLRPIVEFMTWNFAVLALDQILNTASKM
                     LAMSGGQITCPIVFRGPNGSAGQLGAQHSTAFESLYANIPGIKVVSPSNGYDAKGLLK
                     QAIRFEEDPVVFMESEVMYGDKSEVPEEEYYIELGKADVKKIGRDVTIVSFNKMMKVA
                     LGAAVELEKEGISAEVIDLRTIRPLDWMTILESVKKTNRLVIVEEQWPYASVSSEISY
                     RIQKEGFDYLDAPIRRITAADAPLHYAPNLVAAALPDVARTVKLVKEVMYVKK"
     gene            complement(2554..>2782)
                     /locus_tag="PK977_03355"
     CDS             complement(2554..>2782)
                     /locus_tag="PK977_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17172.1"
                     /translation="FSGSGWPQSEKDSFISSCVREAMKGGRSREVASSYCQCMMEKME
                     AEYPDINKAAKLTNDEIEKVMMKYRDGCLSQ"
     assembly_gap    2783..2800
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2801..2986)
                     /locus_tag="PK977_03360"
     CDS             complement(<2801..2986)
                     /locus_tag="PK977_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17173.1"
                     /translation="MKKLLMILMAFSLLTACKDKKEADAKKTETKSADDYRSGDNKTG
                     DDNKGNDDKEKSGVSEDN"
     gene            complement(3083..3736)
                     /locus_tag="PK977_03365"
     CDS             complement(3083..3736)
                     /locus_tag="PK977_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRF17174.1"
                     /translation="MRVRIKICCISSVEEAKLAIKMGADALGLVGKMPSGPGPIADEL
                     IESITRHIHPPVSSFLLTSEQSATAIIQHIHRVNTNTVQIVDELTEGSYSDIRKALPH
                     LGIVQVIHVKGEESVDEALKVHEMIDAILLDSGNPQAAVKTLGGTGNTHNWAISRKIV
                     ESVKVPVFLAGGLHAGNVQQAINEVKPFGVDICSGVRTNGGLDPEKLTAFINAVINV"
     gene            3790..5229
                     /locus_tag="PK977_03370"
     CDS             3790..5229
                     /locus_tag="PK977_03370"
                     /inference="COORDINATES: protein motif:HMM:NF041516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2928 family protein"
                     /protein_id="HRF17175.1"
                     /translation="MRLTARPVIGIIIAAVLIVIGFVYLLKGCLAKYDERFAKPPVLL
                     FEKNGKSVVFVITEFQKTVSYSRKGGMVRKSVNTTYYIQTNDAATAEMIAEKKVKKQR
                     EIKNHPVEIMGASGNNAWLFMNEPMAFDAFTLELKADKAAMEEKNPSLNGKLPDERRY
                     YFFNPADKNIYFTAKDGTKWKLNTQTLMATQEEYDEDQSPLKQQIALLEKAEKKNRED
                     QDSLYQQKNLRPSKQYAAGQITREEFGRIQKIYFEERTLLSKALDSLRVIKRMLEAQE
                     RSGQDLERAIKNLQRGSTGYYNLKINQDTVNGHWFGLYAKNEFDKLYNRMLHQSENDE
                     TARRQLYKSTVSRNSNGDWQIEKEKAALVDGNVSFLHGGFLLDKKTALPVHLAGPDAF
                     LIIHKQEVGQKASILISRVTLEGKILWTFNTKLADWLDWQVNSKQLIITGTNNEELSG
                     NEANVLICIDLVNGKAVGYDYFKRKLISQ"
     gene            complement(5233..>5466)
                     /locus_tag="PK977_03375"
     CDS             complement(5233..>5466)
                     /locus_tag="PK977_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17176.1"
                     /translation="SRPATLATQIDNTLALYKVKHIATGHTVIADTISVLYGGKVFNT
                     DVPHIKGASEGLLVENGKYYRVSPLGEKFQLLD"
     assembly_gap    5467..5482
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5483..>5734)
                     /locus_tag="PK977_03380"
     CDS             complement(<5483..>5734)
                     /locus_tag="PK977_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17177.1"
                     /translation="TLINEPYLALFGENAELGRWLRTKNVSEKIGDFLFVHGGFSSEV
                     NTLDMNLGRLNKLVRPFYGDTSYNYKDPKVDLLYSDLGP"
     assembly_gap    5735..5847
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5848..>6142)
                     /locus_tag="PK977_03385"
     CDS             complement(<5848..>6142)
                     /locus_tag="PK977_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17178.1"
                     /translation="NEKPGITLTVATDIAGKTFPVQLKSTLENEKTEYGKPEKMFVVS
                     DIEGNFAAFRKLLQGNKVIDDNLSWTFGKGHLVLTGDFFDRGSQVTEVLWLIYS"
     assembly_gap    6143..6280
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6449..6895)
                     /locus_tag="PK977_03390"
     CDS             complement(6449..6895)
                     /locus_tag="PK977_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF17179.1"
                     /translation="MGLRQASITDIKQIQQVRNAVKENTLSDPSLVTDDDVMDYITRR
                     GRGWVHEVEGVITGFSIISIIDQNVWALFVHPDFERRGIGRLLHDEMMNWYFANTDKL
                     VWLSTGPGTRAEQFYREAGWQEKVTYGKGEIKFEMRKEDWESGKIS"
     gene            complement(7130..>7939)
                     /locus_tag="PK977_03395"
     CDS             complement(7130..>7939)
                     /locus_tag="PK977_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF17180.1"
                     /translation="KAAITRNLQYIHLVTNAGTTLPTDLWVPSTYLVRPQISWLYALG
                     FFKNLADNTYETSLEVYYKVMQNQVEYAEGYTPSLRDPEEEFVFGKGWSYGAELLVNK
                     LKGRFTGWVGYTLSWTWRRFPDLNEGLKYPTRYDRRHDLSVVTNYELNRKWKFGAVFV
                     YGTGNAITLPERFYFINGVLTQEYSRLNQYRMNAYHRLDLSATYTPQPKKKRKVNSSW
                     VFSVYNTYSRLNPYFIYFDQNGSLANGDLKVEAKQVSLFPIIPAVTWNFKF"
     assembly_gap    7940..7979
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7980..9507)
                     /locus_tag="PK977_03400"
     CDS             complement(<7980..9507)
                     /locus_tag="PK977_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF17181.1"
                     /translation="MLFRYNLLLVVFLLCTKFVLAQSPSKSGRFTLNGYVKDSLSGET
                     IIGATISVNGQSKGVASNQYGFYSITLDEGSYTITASHVSYLGKSVTIDLLENKSYNF
                     DLVSKSASIGEVIVYSRKRDGNVKNAQMGKIDLSINQIRNIPAFMGEVDPLKAIQLLP
                     GVRNAGEGNAGFYVRGGGPDQNLILLDDAVVYNTGHLFGFFSVFNADAIKNVSLIKGG
                     MPAQYGGRLSSVVDVSMKEGNINKFQMEGGIGLIASRFSVQGPIKKNKASFILSARRT
                     YIDALVKPFIKKSSSYYGSGYYFYDVNAKMNYRFSEKDRLYLSGYFGRDVFDFANAKR
                     SFKTNIPWGNSTATLRWNHVFNRRLFSNTTLVYNDYKFKFSAQQENFEIGLSSGIRDG
                     SAKVDFDYYPLPNHKLKFGGLFTYHKFIPNVASGRQDSVVFKPNNEGTKYATETALYI
                     QDDWELGTRWKINYGIRWSTFTQVGPYTRYIRDENLNPLDSTKYKGLEPVKTYGGFEP
                     R"
     gene            complement(9529..10254)
                     /locus_tag="PK977_03405"
     CDS             complement(9529..10254)
                     /locus_tag="PK977_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17182.1"
                     /translation="MKKYIDILGIVLPAIIIVLGIVRVFVKKTKGINGLTMILAILLL
                     ITGLVRYFVFPGNGGSSHSGSKPQPIAVSKHSDIFNNSVEVLLNAYYQLTEGFVNWDT
                     TAINKSGYELRTALDSLKIDELKKDSIIYQTALDPYNNAKIELASILADPSIEEKRGS
                     LNILSDNLRNLLVIVKYDRAKVYWQECPMAFGEERPGNWLSKTDAVRNPYLGNKDPKH
                     GDKMLECGGPKDTINFIVTDTTK"
     assembly_gap    10352..10363
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10364..12320
                     /locus_tag="PK977_03410"
     CDS             <10364..12320
                     /locus_tag="PK977_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRF17183.1"
                     /translation="AGLQNVTFEFGARIIVEDATWHIQPGERIGLIGYNGTGKSTLLK
                     LLVGEYSPSAGTVERSRTTTIGYLHQDLLSFDTNDSILQVALGAFEKILQLEKEIEEL
                     GKELERTGDEKTLHDYSDKLHELETLGGYNIHHRTEEVLQGLGFSNTDLQRPYKEFSG
                     GWRMRVLLAKMILQQPDLLLLDEPTNHLDLPSIEWLEKYLQHYQGSVVIVSHDKYFLN
                     RMVTKIVELYQQQLHIYNGNYDFYEKEKAIRIELQQKAYENQQDYIRQQERLIDRFRA
                     KASKAAMAQSLIKKLDKLERIEDSALERPNIKINFRVDKVPGRVLVELKNVSKAFGSN
                     VIIENASVEIDRGDKIALIGANGKGKSTLLRMIAGVETFEGERKWGHNVEESFYAQHQ
                     LEALNVNNTILDEMRECGSQMTDLELRGLLGCFLFSGDDADKRIKILSGGEKARVALA
                     KVIVSKANFLMLDEPTNHLDMHSCDLLIDALNKYQGSIILVSHDRYFISKTANKIWEI
                     VDHQVKEFKGGYEEWVAWKERMAKNESKSQKAEETAPLVSKTKEDPKPATEPAKQTAS
                     LPINKEAKKELQKMQKQFQQLEEKIARLNTEKSSLEAQLSDPETYSDKQKFLQTEKAY
                     KKAEDELSRCNVEYEKVFERIMELEAK"
     gene            complement(12321..12842)
                     /locus_tag="PK977_03415"
     CDS             complement(12321..12842)
                     /locus_tag="PK977_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17184.1"
                     /translation="MKGYLLLIFLTGIVKVFGQNEFAATAFYNEFKRIYTDATNGFIE
                     NKGSERVSEFPELTKEYKVKMMLPLADSGKIVESAKGSSYVVYFFEPDKVRLKVDQRG
                     AHLRDAILTSVNQPLYARTETLMIDNKPLSNTWYFTDPGETKTAAAAFKLSIYFKEGR
                     YFLSLEIRGKKPS"
     gene            12932..>13761
                     /locus_tag="PK977_03420"
     CDS             12932..>13761
                     /locus_tag="PK977_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17185.1"
                     /translation="MKSPLPFLAVLAFITALLAFRVKEYTSGRKVQTNRYIQSSKTSI
                     GCGPDWASLQQWLEEVSIPPIPGSGKHNWKISTENDSSQFYFNQGINMYYAFHIIEAM
                     ASFKKAVKFDPQSPMLYWAQALTYGPNINDIGYAASPEALTAVEKAIELISGANPKEK
                     MLIKAQQVRYSADSTQSREKLNQLYVDKMKEAYKAFPDDPDVAALYADALMLQHPWDL
                     WGIDGTPRAWTPLIREVLEKILAKAPDHIGANHYYIHVMEPSPYAIKAKPSADRLGLL
                     T"
     assembly_gap    13762..13780
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13781..14684
                     /locus_tag="PK977_03425"
     CDS             <13781..14684
                     /locus_tag="PK977_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17186.1"
                     /translation="SHTVHMPSHIYLRTGEYKNGVLVNEKAVASYKALQSKYAPVSGN
                     DFLYVIHNLHMQTNHAMMTGSSSSAVRAAKQTAESIPKEYLGMQGAMGNYLQYIYMTP
                     VLVNVRYGKWNSLLTMTKPAAGMIYANLLYHFGRGMALAHQNKIADAKKELELLQTLV
                     KDSTLALPFFPFSPAVDGGIVAENILIGTIALKEKRFSDAVAALEEAVVTEENMVYNE
                     PRDWLLNPKHYMGNALLKAGRTKEAKEILMKDLLNNNENGWALFGLWQANIIEKKSAE
                     ANKILSRFRKAFDKADVKLYGPVF"
     gene            complement(14685..15782)
                     /locus_tag="PK977_03430"
     CDS             complement(14685..15782)
                     /locus_tag="PK977_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRF17187.1"
                     /translation="MEKSETKVVAEPRPNHMPHLKIYSKQDILSLTKIRRFETKVGER
                     MHVIYDNSQLERSIADSSAKYVLFGIPEDLGAKGNYGIGGTDTLWIPFLQSFLNIQSN
                     DFLDGNEILIAGHFDFGDIQYLIDTTARGDDEKIEAYRHAVNTIDDEVEHLVKIITEA
                     KKIPIVIGGGHNNSYPLIKGAAKGWHKTGVIPLAQINCINLDAHADYRPMEGRHSGNA
                     FRYAEEDGYLAKYCVVGIHENYIPQNVWIDIVNNPFIDCITYEDIFVHDKRTFLQAVA
                     HATGFTEDGLCGIDIDIDCIEHTLSSAMTPVGILPNHARQYVSFVAADSKPAYFYICE
                     GATRLNDGRTDPTSGKLISYLVSDFIKGSAV"
     gene            complement(15763..17415)
                     /gene="ftcD"
                     /locus_tag="PK977_03435"
     CDS             complement(15763..17415)
                     /gene="ftcD"
                     /locus_tag="PK977_03435"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRF17188.1"
                     /translation="MQPLIECVPNFSEGADLVIIKQITDQVEMVEGVRLLNVDPGKAT
                     NRTVVTFVGNPEAVIEAAFLAIKKAGELIDMSRHKGEHPRMGATDVCPLIPISGISME
                     ETAQYAQRLAKRVGEELHIPVYLYEAAQPDKKRSNLSVIRAGEYEGFFKKIKQPEWKP
                     DFGPAEFDIKRGGTVIGARDFLVAYNVNLNTTSTRRANAIAFDVREAGRNVVENGVKV
                     NKPGSLKSVKAIGWYIEEYGIAQISMNLTNINVTPIHIAFDEVCRKADARGIRVTGSE
                     LVGLVPLKALTDAGKYFLRKQKRSTGVSEKELIKIAVKSMGLDELGPFKPEERIIEYM
                     LRNKADTKLVTMTLSDFADETASESPAPGGGSISAYVGALGVSLATMVANLSSHKKGW
                     DDRWDEFSNWAEKGEALKSELVRLVDADTKAFNQIMSAFGLPKSTDEEKAARSKAIQE
                     ATKFAIEIPFKVMQIAYASMEVIKAMADTGNPNSVSDAGVGALCARSAVMGAFMNVRI
                     NAAGYEDKAYVTNILNKGREIENNAIALEAEILKIVDGKIGN"
     gene            18137..>18679
                     /locus_tag="PK977_03440"
     CDS             18137..>18679
                     /locus_tag="PK977_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRF17189.1"
                     /translation="MKKYIRTDNKSAYQKAKQYRESRISIKGHVRDFVLISIGIFSAA
                     FGLKGFLLTNKFIDGGATGVSLLVSALTQTPLYLLIICINIPFMVLGYRILGKQFAIK
                     TALAITGLALVIANIEFPNVTDDNLLVAVFGGFFLGAGIGFSIRGGAVIDGTEVLAIF
                     LSRKFGVTIGDIIIVINAIIF"
BASE COUNT         5448 a         4046 c         3485 g         5344 t
ORIGIN      
        1 tcattcaatt gatgaaatgc agaattttgc tcggaatatg gctaaaacta cttaggcata
       61 acatagcttc ttatatcttc ggcagtgata gttttgcccg aaagaataac aagtctttcc
      121 acaacattgc gcagttcccg tatattaccc gtccagttat attcatgcag caattttatg
      181 gcttcctctg ctatcggctt tttggcaata ccatattcac tgcagatatc ctgcaagaat
      241 ttatcgacca gcaacggtat atcatctctc cggtcgttta aagacggtac atggataaga
      301 atcacactaa gacgatgata taagtccaaa cggaaatttt tggcttcgac ttctttcaga
      361 agatctttat tagttgccgc tatcaccctt acatccacac taatgtcttt atcggctcct
      421 acccttgtta tctttccttc ctgcaaggcc ctgagcactt tagcctgggc attggcactc
      481 atatcaccga tttcatccag aaaaagtgtg ccgccattgg ccgattcaaa ttttccgatc
      541 ctttgtttga tggctgaagt gaaggagcct ttttcatgac cgaataactc actttcgatc
      601 agttcagtgg gaatggctgc acaattcacc tctattattg ggccggaagc cctgttactc
      661 ttctcatgaa tccatcttgc cactaattcc ttacctacgc cattctcccc ggttatgagg
      721 atccgggcat ccgttggggc aactttttca atcgtttcct taatcttttt tatgggtaca
      781 gattctccga tcatttcctg tacacggctc acttttcttt tcagcacttt tgtctccgtt
      841 acaaggctgc ttttgtccat cgcattgcgg atagtgatca gcaggcggtt aagatctggt
      901 ggttttgaga tgtaatcata agcgcctttt tttacagctt ccacagctgt ttcaatattg
      961 ccatgccctg aaatcataat taccggaaca tctgcattga tttcaccagc cttttgcaga
     1021 aactcaatgc catccagttt gggcatcttt atatcacaaa gtacaaggtc aaagttcttc
     1081 tctttaaatt ttttcaggcc ttcttctccg tcagctgctt cttcaatttt gtaaccttca
     1141 aaagaaagaa tctcagtcag cgttttgcgg atagctttct catcatctat aatcagaatg
     1201 tctgccatat tattgaattt gggtaacaaa aatagctgat ttgctatgaa gtgaaggatt
     1261 cgcttaaaat gcaaaaaata aaggccgggt agaaacccag cctcgtttta aaatccaata
     1321 tcctatgaaa aaccgttgca aagatgcagc ttcttcgtca aaacgttgca ccctaaccga
     1381 aaaatattat tgtgtttgtc gcaaaaaata accccgggaa aaccggggtt tattataaat
     1441 aagttatagt tctcaattat ttttttacat acatcacttc ttttaccagc ttcaccgttc
     1501 tggccacatc cggcagtgca gcagccacca ggtttggcgc atagtgcagc ggtgcatctg
     1561 ccgctgtaat acgacggatg ggcgcatcaa ggtaatcaaa tccttccttt tgtatacgat
     1621 agcttatctc tgaggaaaca gaagcataag gccattgttc ttctacgatt accagcctgt
     1681 tggttttctt tacactttct aaaatggtca tccagtcaag cggacgaata gtgcggagat
     1741 caattacctc agcgctgata ccttcttttt ccagttcaac tgcagcacca agcgccactt
     1801 tcatcatttt gttgaaagaa acaatagtca catcacggcc gatctttttc acatcagctt
     1861 tgcctagttc aatataatat tcctcttccg gcacttcact tttatcgcca tacataactt
     1921 cactttccat aaaaacaacc gggtcttctt caaaacggat tgcttgtttc aataaacctt
     1981 ttgcatcata tccattgctg ggagatacaa ccttaatacc gggaatattg gcatacaggc
     2041 tttcaaaagc ggttgaatgt tgtgccccaa gctgaccggc tgaaccgttt ggcccgcgga
     2101 aaacaatagg acaggtaatt tgtccaccac tcattgccag cattttagaa gccgtattga
     2161 gaatttgatc caaggctaaa acggcaaagt tccaagtcat aaactctact ataggcctta
     2221 gcccattttg cgctgcacct acacctacag ccgcaaagcc aagctcagaa ataggagtat
     2281 caattactct ccttgggcca aattctgcca gcataccctg gcttacttta tatgcgccat
     2341 tatactctgc cacctcttcc cccattagga aaaccctgtc atctcttctc atttcctcag
     2401 tcatcgcttc acgaagtgct tcccggaatg ttaataatct tgccatttaa gatagaattt
     2461 ttggaggggc aaatttaagc cggtgacagg ataaaaccta aaagtaaaaa gcccgcaaca
     2521 aaggctgcag gctaaacaat ttctttcaaa actctattgc gaaagacagc cgtccctgta
     2581 tttcatcatc accttttcaa tttcatcatt ggtcagtttg gcagccttat taatatccgg
     2641 atactcggct tccatctttt ccatcataca ctggcaataa ctggaagcta cttccctgct
     2701 gcggccacct ttcatggcct cccttacaca gctactgata aaactatcct tttcgctttg
     2761 cggccagccg ctaccggaga agnnnnnnnn nnnnnnnnnn gttatcttct gaaacaccgc
     2821 ttttttcttt atcatcgtta cccttattgt catcacccgt tttgttgtca ccgcttcggt
     2881 aatcatctgc agatttggtt tccgtctttt tggcatcagc ttccttttta tccttacaag
     2941 ctgtcaaaag gctgaacgcc attagtatca ttagtaattt tttcatgttc tcaatggttt
     3001 ttgatgaagt cgagttaagt aaacaatttc attacaatgt agatattctt tcccagtaag
     3061 gaaaattcag atacccaaag ttttaaacat tgattacggc attaataaaa gcagttaatt
     3121 tctccggatc aagcccccca tttgttctta caccgctgca tatgtccaca ccaaatggct
     3181 ttacttcatt gattgcctgt tgtacatttc cagcatgtaa gccgcctgcc aggaataccg
     3241 gcactttaac gctttcaact atcttccggc tgatggccca attatgtgtg ttaccggtac
     3301 cacctaaggt tttgacggca gcctgtggat tcccgctgtc cagcaaaata gcatcaatca
     3361 tttcatgtac cttaagcgcc tcatcaacac tttcttctcc ttttacatga ataacctgta
     3421 ctatacccag gtgaggcagc gctttacgaa tatcactata tgacccctct gtaagttcat
     3481 ctactatttg gacagtattt gtattaactc tatgtatatg ctgtataata gctgttgcag
     3541 attgctcact ggtcagcagg aaagaggaaa ccggagggtg aatatgccgg gttattgatt
     3601 caatgagttc gtctgcaata ggcccgggtc cgctgggcat cttacccacc agccccaatg
     3661 catcggcacc cattttaata gccagcttcg cttcttcaac cgaagagata caacatattt
     3721 ttattcttac ccgcatttat caaagataag ctggccgttt tttgtatttt tcaaaaaact
     3781 acctgtttta tgcgactaac tgccaggcct gttattggaa tcattattgc ggctgtatta
     3841 attgttattg gctttgtata cctgttaaaa ggttgtctag caaaatatga tgaaagattt
     3901 gccaaaccac cggttctgtt atttgagaag aatggaaaat ccgttgtatt cgtcataaca
     3961 gaatttcaga aaacagtttc ctattcaagg aaaggtggaa tggtcaggaa atctgtcaat
     4021 acaacttatt atatccagac aaacgatgcg gctacagctg agatgatcgc agaaaaaaag
     4081 gtaaaaaagc aaagagagat caaaaaccat ccggtagaaa taatgggagc ttccggcaac
     4141 aatgcctggc tatttatgaa tgagccaatg gcttttgatg cattcacact tgagctgaaa
     4201 gccgataaag cagcaatgga agaaaaaaat ccttccctga atggcaaatt accggatgaa
     4261 agaagatatt attttttcaa tccggctgac aagaacatct actttactgc taaagatggt
     4321 acgaaatgga aattaaatac acaaacactg atggcaaccc aggaagaata tgatgaagat
     4381 caaagtccgc tgaaacaaca gattgccctg ctcgaaaagg cagaaaaaaa gaaccgggaa
     4441 gatcaggatt cattatatca gcaaaaaaat ttacggccgt caaagcaata tgctgccggt
     4501 cagataacaa gagaagaatt tggacgcatt cagaaaatct attttgaaga aagaacctta
     4561 ttaagcaaag cacttgacag cctccgggtt attaaacgaa tgctggaagc acaggaacga
     4621 tccggccagg atcttgaaag ggcaataaaa aacctgcaga gggggagcac tggttattac
     4681 aacctaaaaa ttaaccagga tacagtgaat ggccattggt ttggcttata cgcaaagaat
     4741 gaatttgata aactgtataa ccgcatgctg caccaaagcg aaaatgatga gacagcccgt
     4801 cgccagttat acaaatcaac agtcagcaga aattctaatg gtgactggca aatagaaaaa
     4861 gagaaagctg cccttgtgga tggcaatgtc tcttttttac atggcgggtt tttactggat
     4921 aaaaaaacag ccttgcccgt tcaccttgca ggcccggatg catttttgat tattcataaa
     4981 caagaggtag gtcagaaagc ctcaatcctg atcagcaggg tgacactgga aggaaaaatt
     5041 ctatggacat ttaacaccaa acttgcagat tggctcgact ggcaggtaaa cagcaagcaa
     5101 ctaataataa cagggactaa taatgaggaa ctgagtggca acgaagccaa tgttttgatt
     5161 tgtattgacc ttgtcaacgg taaagctgtg gggtatgatt attttaaaag aaagctgatc
     5221 agtcaataac cattaatcca gtaactgaaa tttttcgccc agcggactaa ccctgtagta
     5281 cttgccattc tccacaagta atccctcaga ggctcccttt atatgcggca catctgtatt
     5341 aaaaacctta ccaccatata atacactgat tgtatctgcg atcaccgtat gaccagttgc
     5401 gatatgtttt actttataca gcgccaaggt attgtcaatc tgggttgcta atgtggcagg
     5461 cctgctnnnn nnnnnnnnnn nnaaagggcc caggtcgcta tagagcaaat ctacctttgg
     5521 atccttgtaa ttataggatg tatcgccata gaatggccgt accaatttgt taagccgtcc
     5581 aagattcata tccaatgtgt tcacttccga agagaaaccg ccatgcacaa acaggaaatc
     5641 tccgattttt tccgatacat ttttagttct taaccagcgg ccgagttcag cgttttcacc
     5701 aaacaacgca aggtaaggct cattaataag cgtannnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa tagataagcc agagtacttc tgtaacctga
     5881 gaacctctgt caaaaaaatc acctgtcagc accaggtgcc cctttccaaa agtccagctg
     5941 aggttatcgt caattacttt atttccctga agtagtttac ggaaagccgc aaaattgccc
     6001 tcgatatcag aaaccacgaa cattttctcc ggtttgccat attctgtctt ttcattctcc
     6061 aatgttgatt ttagttgtac cggaaatgtt ttgcccgcaa tatcagttgc cactgtcaaa
     6121 gtaatacccg gtttctcgtt atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccctgcttct gaagggtatc
     6301 agtaatcaat aacccggaaa caaaaagcga aaacaatggc aaaatcttca ttttcataaa
     6361 acctgttatt tagctattct cttttcagac agagccggcg ataaataggt tgccagcaag
     6421 gttaaacatt aaagatattg taaaactttc aactgatttt tcctgactcc cagtcttctt
     6481 ttctcatttc aaattttatc tcccccttac cgtaagttac tttctcctgc cagcctgcct
     6541 ctcggtaaaa ttgctcagct cttgttccgg ggccagtact taaccaaacc agtttatcag
     6601 tgtttgcaaa gtaccaattc atcatttcat catgcagtaa tctgccaata ccccttcttt
     6661 caaaatcagg atgtacaaaa agtgcccaga cgttttgatc aattatcgaa atgatagaaa
     6721 agcctgtgat gaccccttct acttcatgca cccaacccct tccccgcctg gtgatatagt
     6781 ccattacatc atcatcggtt accagggatg gatcagataa ggtattttcc tttacagcat
     6841 ttcttacttg ctgtatttgt tttatatccg taatagatgc ttgtcttaat cccatttata
     6901 acattttcaa attaaatatt acatcaatga ataatattaa gtgtaaaatc tgatagaaga
     6961 tggttataaa tactcacaat aacaaaaatc aattttatac tacaaaacta ttgaccctta
     7021 tcagcatgta cttttggata tacttattat attgccgagt aaacaatttt aaattcggat
     7081 taattcgtaa aaaggcctgt gtcaacaggc tttttttata gaggattaac taaaacttga
     7141 aattccaggt tactgccgga atgatgggaa ataaggatac ctgctttgct tcaactttca
     7201 aatccccgtt agcaagactt ccgttctgat cgaaataaat aaaataagga tttaaacggc
     7261 tataggtgtt gtaaacgcta aagacccatg aactgttaac ctttcttttt ttcttaggtt
     7321 ggggcgtata ggtagcagac agatcaagac gatgataagc attcatccgg tattggttta
     7381 accggctgta ttcctgggtt agtacaccat taataaaata aaaacgttcc ggtaatgtaa
     7441 tagcattacc agtgccataa acaaatacag ccccgaattt ccatttcctg tttagttcat
     7501 agttggtaac aacagaaagg tcatggcgac gatcatatct tgtcggatat ttcaaaccct
     7561 cgttcaaatc aggaaatctt ctccaggtcc atgacaacgt ataacctacc cacccagtaa
     7621 accgcccttt taatttattg accagcaatt cagcaccata gctccaacct tttccaaaaa
     7681 cgaactcctc ctcaggatcc cggagtgacg gagtgtatcc ttctgcatat tccacctggt
     7741 tttgcataac cttgtaatac acttccaatg aggtctcgta agtattatca gcaagattct
     7801 taaagaatcc caatgcatac agccagctta tttgcggcct taccaggtaa gtactaggta
     7861 cccagagatc agttggcaat gtcgtacctg cattggttac aagatgaata tactgcaggt
     7921 ttcttgttat agcagccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     7981 ccttggttca aaaccgccat aggttttaac cggttcaagc cccttatatt ttgtgctgtc
     8041 aagcggattc aggttttcgt cccggatata tcttgtataa ggtccgactt gtgtaaaggt
     8101 gctccaacgg ataccgtaat ttattttcca tcgggtaccc agttcccagt catcctgtat
     8161 atacaaagct gtttctgtgg catattttgt tccttcatta ttgggtttga aaacaacaga
     8221 atcctgacga ccgcttgcca cattgggaat aaatttgtga taagtaaaaa gtccgccaaa
     8281 tttcaattta tgattgggta acggataata atcaaaatct actttggcag agccgtcacg
     8341 gatacccgag cttaagccaa tttcaaaatt ctcctgctga gcagaaaatt tgaacttata
     8401 atcattatac acaagtgttg tattggaaaa aagtcttctg ttgaatacat gattccaccg
     8461 taatgtggct gtagaatttc cccagggaat atttgtttta aaagaacgtt tggcattggc
     8521 aaaatcgaat acatcccttc cgaaataacc gctcaggtaa agccggtcct tttcagagaa
     8581 ccggtaattc attttagcat ttacatcata aaagtaatag cccgacccat aatagctgct
     8641 gctcttctta atgaaaggtt ttaccagcgc atcaatgtag gtgcgacggg ctgacaaaat
     8701 gaatgatgct ttattcttct taattgggcc ctgcaccgaa aatcttgatg caatcaaacc
     8761 aatacctcct tccatttgaa atttattgat attaccctct ttcatagaaa catcaactac
     8821 agaagaaaga cggccaccat attgggccgg cataccaccc tttatgagtg atacattttt
     8881 aatcgcatcc gcattaaaga cagaaaaaaa accaaacaaa tgacctgtat tatacaccac
     8941 agcatcatcc agtaagatca ggttctgatc aggtcccccg ccccttacat aaaaacctgc
     9001 attgccctcg cctgcatttc ttactcccgg tagcagttgt atagctttga gcggatctac
     9061 ttctcccata aaagcaggaa tattcctgat ctggttgatg gaaagatcaa ttttacccat
     9121 ttgcgcattt ttcacattcc catctctttt acggctatac actatgactt ctccaatgga
     9181 agctgatttg gaaacaagat caaaattgta ggatttgttt tccagcaggt caatagtgac
     9241 agatttgccc aaataagata catgtgaggc ggtgatggta taactgcctt catccagtgt
     9301 aatagaatag aatccatatt gattactggc tacacctttg gattgtccgt ttacactgat
     9361 tgtagcgcca ataatagtct ctccagacaa actgtctttt acataaccat tgagggtgaa
     9421 gcggccggat tttgatggag attgcgccag tacaaatttt gtacacagta agaaaactac
     9481 caggagtaaa ttgtaccgga aaagcatacg gctattacaa tctgcttatt attttgttgt
     9541 atcagtcact ataaaattga tcgtatcctt aggtccgccg cattctagca ttttatcccc
     9601 atgtttgggg tctttattac cgagataggg atttcgtact gcatctgttt tgctcagcca
     9661 gttgccgggt ctttcttcgc caaaagccat tgggcactcc tgccagtaca ctttggcacg
     9721 gtcatacttt acgattacca gtaggttgcg gagattgtct gacaatatat ttaatgaccc
     9781 cctcttctct tcaattgacg gatcggctag tatcgaagcc agttcaattt ttgcattatt
     9841 gtaagggtcc agtgcggttt gataaatgat tgagtctttt ttcaattcat cgatctttag
     9901 gctgtccagt gcagtacgca actcatagcc ggacttgttt atagctgttg tatcccaatt
     9961 aacaaaaccc tcagtcaact ggtaataggc attcaataaa acctcaactg agttattaaa
    10021 aatatcactg tgcttgctca ctgctatcgg ttgtggtttg ctgccggaat ggctggagcc
    10081 tccgttgcct ggaaaaacaa aatagcgaac aaggcctgtt ataagtaaaa gtattgccaa
    10141 aatcatcgtc aggccattga tcccttttgt cttttttaca aacacccgta caatacctaa
    10201 aacaatgata atggcaggaa gcacaattcc gagaatgtcg atatattttt tcatagtgaa
    10261 agtttactgg ccctggggct tctgcaaaga taatcggcca ttcggcaagt actaatccat
    10321 tatttttgca aactgttaaa atgaaaaaga annnnnnnnn nnnggcaggt ttacaaaatg
    10381 tgacttttga gtttggcgca agaataattg tggaagatgc tacctggcat attcagccgg
    10441 gtgaacgtat cggcctgata ggctacaatg gtaccggtaa atccaccctt ctaaaactat
    10501 tggttggcga gtactcccct tctgccggca ccgtggaaag gagccgcacc actaccattg
    10561 gttacctgca ccaggacctg ctgagcttcg acacaaatga ttccatcctg caagtagcct
    10621 tgggtgcttt tgaaaaaatt ttacagctgg aaaaagaaat tgaggaactg ggtaaagaac
    10681 tggaaagaac tggtgatgag aaaacgctgc acgactatag cgataaactg catgaattag
    10741 aaacgctggg aggctacaat attcaccacc gcacagaaga agtattgcag ggattaggtt
    10801 tttctaatac cgaccttcaa agaccttata aggaattcag cggtggctgg cgaatgaggg
    10861 tactattggc aaaaatgatc ctgcaacaac ctgacctttt gctcctggat gaaccaacaa
    10921 atcacctcga tcttccatcc atcgaatggc tggaaaaata tttgcagcat taccagggtt
    10981 cagtagtaat tgtgagtcac gataagtact tcttaaaccg gatggtgacg aagatcgtgg
    11041 agttgtacca gcaacagtta catatctaca acggcaacta tgatttctac gagaaagaaa
    11101 aggccattcg gattgaatta cagcaaaagg cttatgaaaa ccagcaggac tatatacgac
    11161 aacaagaacg actgatcgac cggttcaggg ccaaggccag taaagctgcc atggcgcaaa
    11221 gcctgatcaa aaaactggac aaactggaac gcatcgaaga ctcggcactg gaaaggccaa
    11281 atattaaaat aaatttcagg gttgataaag taccgggtag agtacttgtg gaattaaaaa
    11341 atgtaagcaa agcttttggc agtaatgtta taattgaaaa tgcttccgta gaaattgacc
    11401 ggggcgataa aattgccctg atcggtgcca acggtaaggg taaatcaacg ttattgcgga
    11461 tgatcgctgg tgttgaaaca tttgaaggag aaagaaaatg gggacacaat gtagaggaaa
    11521 gcttttatgc gcagcatcaa ttggaagcac taaatgtaaa taataccatc cttgatgaaa
    11581 tgagagaatg cggcagccaa atgactgatc tggagttgcg tggcttgttg ggctgttttc
    11641 ttttcagcgg ggacgatgca gacaaaagga taaaaatatt aagtggggga gaaaaagcaa
    11701 gggtggcact ggcaaaagtt attgtaagca aagctaattt cctgatgctg gatgaaccga
    11761 ccaaccacct tgacatgcac agttgcgact tacttattga tgctttgaac aaataccagg
    11821 ggagcattat actcgtaagc cacgaccgct atttcatttc taaaacagcc aataaaattt
    11881 gggagatcgt tgatcaccag gtaaaggagt ttaaaggtgg ctacgaagaa tgggtggcct
    11941 ggaaagaacg gatggcaaag aacgaatcaa aaagtcagaa agcagaagaa accgcaccac
    12001 tggttagtaa aacaaaagaa gacccaaagc ctgctactga acctgcaaaa caaactgcat
    12061 ctttacctat aaacaaagag gcaaaaaaag aattgcaaaa aatgcaaaag cagtttcagc
    12121 aactggaaga aaagatagcc aggctcaata cagaaaaatc atcacttgaa gctcagttgt
    12181 cagacccgga aacctattca gacaagcaaa aatttttaca aacagaaaaa gcctacaaaa
    12241 aggctgaaga tgaattgagt cgctgtaacg tagaatatga aaaagtattt gaacgaatca
    12301 tggaactgga agcaaaatga tcaagatggt ttcttacctc ttatttccag cgaaagaaaa
    12361 tacctaccct ctttaaaata aatcgatagt ttgaatgcag cagcagctgt ttttgtttca
    12421 cccggatcgg taaaatacca ggtattggaa agcggcttat tgtctatcat cagggtttca
    12481 gttcttgcgt ataaaggctg atttactgaa gtaagtattg catcccgcag atgggcccct
    12541 ctttggtcta ctttaagcct tactttatcg ggttcaaaaa aataaactac ataggaactg
    12601 cctttagcag attcaacaat ctttcctgaa tctgccaaag gcaacatcat ttttacttta
    12661 tactctttgg taagctccgg aaactcactc accctttcac tgcccttgtt ttcaatgaaa
    12721 ccgtttgtgg catcagtata tattcttttg aattcattgt aaaaagctgt tgcagcaaat
    12781 tcattctgtc cgaaaacttt tacaatcccg gttaaaaaga taagtaaaag gtagcctttc
    12841 ataacataaa aatactcacc ttgtccgaaa gaaaataaaa agatgatttt cttacaaaat
    12901 ctgtaccttt tatgaacaca aactaacagt tatgaaatca cctctgccat ttctggcagt
    12961 gctggccttc atcacagcat tgctggcctt ccgtgttaaa gagtatacat ccggaagaaa
    13021 agtacagact aaccgctata ttcaatcttc aaaaaccagt atcggctgtg gtcctgattg
    13081 ggcatcttta cagcaatggc tggaagaagt atccattccc cctatacccg gatcgggcaa
    13141 acacaattgg aagattagta cagagaatga tagtagtcaa ttctatttta accagggcat
    13201 taatatgtac tatgcttttc atattattga agcaatggca tcttttaaaa aagcggtaaa
    13261 gtttgaccct caaagcccca tgctttactg ggcacaggct ttaacctatg gccccaatat
    13321 caatgatata ggttatgcag cttcgccaga agcattaacg gctgttgaaa aagctattga
    13381 gctgatctca ggggcaaatc caaaagaaaa aatgctaatc aaagcacaac aagtacgtta
    13441 ttcagccgat tctacccagt caagagaaaa actaaaccag ttatacgttg ataaaatgaa
    13501 agaggcatac aaagcctttc ctgatgatcc cgatgttgct gccttgtatg ctgatgcgct
    13561 gatgctgcaa catccctggg atttatgggg cattgacggc acacccagag catggacgcc
    13621 attaataaga gaagtactgg aaaaaatatt agccaaagca ccagaccata taggagccaa
    13681 ccattattat attcatgtaa tggagccttc gccctatgca ataaaagcaa aacccagtgc
    13741 agacaggctg ggcctgctta cnnnnnnnnn nnnnnnnnnn ttcacatacc gttcatatgc
    13801 cctcgcatat ttatttaaga accggcgaat ataaaaatgg ggtattggta aatgaaaaag
    13861 cagtagccag ctataaagcc cttcaatcta aatatgcgcc tgtaagtggt aatgattttt
    13921 tatatgtcat ccacaatttg catatgcaga caaatcatgc catgatgacc ggatcatcct
    13981 cttctgcggt gcgagcagcc aaacaaacag cagaaagcat acctaaagag tacctgggca
    14041 tgcagggagc catgggaaat tacctgcaat atatttatat gacacctgtt cttgtcaatg
    14101 ttcgttatgg caagtggaac agtttattaa ccatgaccaa gcctgcggcc ggaatgattt
    14161 acgctaacct tctttaccat tttggcaggg gaatggcatt ggcacaccaa aacaaaatag
    14221 ctgatgcaaa aaaagagctt gagctcttgc agacactagt taaagacagc acattggcac
    14281 ttcccttttt tcctttttct ccggctgttg atggtggcat agttgctgaa aacattctta
    14341 ttggaactat agcgctgaaa gaaaaaagat tcagtgacgc tgttgctgcg ttggaagaag
    14401 ctgtggtcac tgaagaaaat atggtgtaca acgaaccaag agactggctg ctcaatccaa
    14461 agcactacat gggtaatgca cttctaaaag ccgggaggac gaaagaagca aaagaaattt
    14521 taatgaagga tctattgaac aataatgaga atggctgggc cctttttggg ttatggcagg
    14581 caaatattat cgagaaaaaa tcagcagaag caaataaaat actaagccgg tttagaaaag
    14641 cctttgataa agcagacgtc aaactttacg gcccggtatt ttaatcagac agcacttcct
    14701 ttgataaagt cactaaccag gtaactgatc aatttaccac tggtaggatc tgtacgtccg
    14761 tcatttaacc ttgtagcccc ttcacagata tagaaataag ccggtttgct atctgctgcc
    14821 acaaaagaca catattgcct ggcatgattg ggcaagatac ctaccggtgt catggcactg
    14881 ctaagtgtgt gttctataca atcaatgtcg atatcaatac cacagagacc gtcttccgtg
    14941 aaaccagtag catgggccac agcctgtaaa aaagttcttt tgtcatgcac aaatatgtct
    15001 tcgtaggtaa tgcagtcgat aaaaggatta tttacaatat cgatccatac attctggggt
    15061 atataatttt catgtatccc gaccacacaa tatttagcaa ggtatccgtc ttcctcagca
    15121 tatcgaaagg cattgccact atggcggcct tccatcgggc ggtagtcggc atgagcatcc
    15181 aggttaatac agttgatctg tgccaatggg atcactccgg ttttatgcca gcctttggct
    15241 gctcccttta tgagaggata agagttatta tgccctcctc ctataacaat gggaattttt
    15301 ttggcctctg taataatctt caccaggtgc tcaacctcat catcaatagt attcaccgca
    15361 tggcgataag cttcgatctt ttcatcatct ccccgggctg tggtatcaat caggtattga
    15421 atatcaccaa aatcaaaatg gccagctatc aaaatttcat tcccgtctaa aaaatcatta
    15481 ctctgtatat tcaggaagct ttgcaaaaaa ggaatccata atgtatctgt tcccccaata
    15541 ccatagtttc ccttggcgcc caaatcttcg ggaataccaa acagcacata tttagccgaa
    15601 gaatcggcaa tggatctctc cagttgggag ttatcataga taacatgcat ccgttcacca
    15661 accttggttt caaaacggcg gatttttgtc aatgacaaaa tatcctgctt ggaataaatt
    15721 ttcagatgtg gcatatggtt gggccttggc tcggcaacaa ccttagtttc cgatttttcc
    15781 atcaacaatt tttaaaattt cagcttctaa agctatggca ttgttctcaa tttccctgcc
    15841 cttgtttagt atgttcgtta cgtatgcctt atcctcataa ccggctgcat tgatgcgtac
    15901 attcataaaa gcccccatta ctgcactgcg ggcacatagg gctcctaccc ctgcatctga
    15961 aacagaattg ggattaccgg tatcggccat cgcctttatt acttccatac tggcataagc
    16021 tatttgcatc accttaaagg gaatttcaat ggcaaatttc gtagcctcct gaatggcctt
    16081 tgaccttgca gctttttctt catcggttga tttaggtaac ccaaatgcag acatgatctg
    16141 gttaaatgct tttgtatccg catccaccag tcgcaccagt tcacttttta atgcttcgcc
    16201 tttttctgcc cagttactaa attcatccca tcgatcatcc catccctttt tgtgagaaga
    16261 gagattggcc accatcgttg ccagggatac acccaaagcg ccgacatatg ctgaaataga
    16321 tccacctccc ggtgcaggac tttcactggc cgtttcatct gcaaaatcac tcagtgtcat
    16381 agtcaccagt ttggtgtcgg ctttattacg aagcatgtat tcaataattc tctcttctgg
    16441 tttaaatggc cccagttcat ccagccccat acttttgacc gctatcttga tcagttcttt
    16501 ttcagaaacc cctgttgatc tcttttgctt tctcaaaaaa tatttacccg catctgtcag
    16561 tgctttcaat ggcaccaatc ctactaattc acttcctgtt acacggatac cccttgcatc
    16621 tgctttacgg catacttcat caaaagcaat atgaatcggt gttacattta tatttgtcag
    16681 gttcatactg atctgtgcaa ttccgtattc ttcaatatac cagccgattg cctttaccga
    16741 ttttaaagat ccgggcttat ttactttcac tccattctcc actacatttc ttccggcctc
    16801 tcttacatca aaggcaatag cattggccct tcttgtggaa gtagtgttaa ggtttacatt
    16861 ataggctaca agaaaatccc ttgcccctat cacagtaccg cctcttttga tatcaaattc
    16921 tgcaggccca aaatcaggtt tccattccgg ctgtttgatt tttttaaaga atccttcata
    16981 ctcccctgcc cttatcaccg ataaattact tctcttctta tcaggttgtg ccgcttcata
    17041 caggtaaaca ggtatatgta attcctcacc cactcgtttc gccagccttt gcgcatactg
    17101 tgcagtttcc tccatagata tacctgatat tgggatcagc ggacaaacat cggtagcccc
    17161 cattcgtgga tgctcccctt tatgcctgct catatcaatc agttcccctg ctttttttat
    17221 agcaagaaaa gctgcttcaa tcacagcttc cggatttccc acaaatgtta ctaccgttct
    17281 gttcgttgcc tttccggggt cgacatttag taaacgaaca ccttctacca tttctacctg
    17341 gtccgttatc tgtttaataa tgacaaggtc agctccttca ctaaaattgg gtacacattc
    17401 tattagcggt tgcatgtaaa tgaaatttga gctaagatat tgattaacca attagtagat
    17461 aatatataca gaatgatttt cattttagaa ccccaaatac agatgcctta tcttcgcaac
    17521 catgctaaaa aagcccggca tattcttttt cttgctgttt attctgacag ccatcgctgg
    17581 ttttagtaca aagcaatccg gggctgaaaa taccaacgta aataagaaga aacttgtcct
    17641 aaagacattt catctgccgc atgcagaact ggagaggccg caaacatcaa gtgtcgtttt
    17701 cggtcatgcc caggggcacc gcactatcaa tttttctatt catacatgtt acccagtatt
    17761 aaggtttttt ttagaagctg tttcaaagac taaaacaagt aaaaaaacct tcccctcgaa
    17821 agaatacctg tattacatct atccttccca taatttctgg taagcaattt tcaatttgac
    17881 tgatctgtaa acatcgtgta catcttgtac aatggtgcat ttttcattct taaaaaagtt
    17941 agtcatgaat gaaacgctcc tggctgtact tgcactgctt ggcgggttat ttgcaatcat
    18001 atcattgctg gtatatgtga acaaccgtaa caaaagaaga gatgcagata aggataaaca
    18061 ataaccctat cggcagagcc agtataatgg attctgccct tgatttcaac ccgtaaaaaa
    18121 attaaaaaag acttgcatga agaaatatat caggactgat aacaaatctg cttatcaaaa
    18181 agccaaacaa tacagagaat cccggatctc aatcaagggg catgtacggg actttgtgct
    18241 tatttccatt ggtatatttt cagctgcctt tggcttaaaa ggctttttgc ttacaaacaa
    18301 gtttatagat ggtggggcca ccggtgtatc actgcttgtt tcagccctga cccaaacacc
    18361 gctctatctc cttatcattt gtataaacat tccctttatg gtacttgggt ataggatatt
    18421 aggaaagcaa tttgccatta aaacggcgct ggccatcacc gggctggcat tggtcattgc
    18481 taacattgag ttcccaaatg taaccgatga caatttgctt gttgcggtat ttggaggttt
    18541 ttttctaggc gctggcattg gcttttctat tcgtggtggt gctgtaattg atggtactga
    18601 ggtgcttgct atctttctca gccgaaaatt tggtgttaca attggcgata tcatcatagt
    18661 tattaatgcc attatattt
//
LOCUS       DAORLS010000024        17663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1717_length_17663_cov_2.203998, whole genome shotgun sequence.
ACCESSION   DAORLS010000024 DAORLS010000000
VERSION     DAORLS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1717_length_17663_cov_2.203998"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>245
                     /locus_tag="PK977_03445"
     CDS             <1..>245
                     /locus_tag="PK977_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF17190.1"
                     /translation="IDAVGKHKNIEFIKHCFVVDIITQHHLGYLITKSTPDIECYGVY
                     VLNLETNKIEKILAKTTLLATGGNGQVYRTTTNPFIA"
     assembly_gap    246..327
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <328..1196
                     /locus_tag="PK977_03450"
     CDS             <328..1196
                     /locus_tag="PK977_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF17191.1"
                     /translation="TALYEPGVGGQAFLITEAVRGDGGILRNKKGEAFMEKYDPRKDL
                     APRDIVARAIDSEMKKTGTEHVWLDCRHFDKEKFIEHFPNIYQKCMSIGIDITQHMIP
                     VAPAAHYSCGGIKTDEWGRSSIKNLYACGECASTGLHGANRLASNSLLEAMVFAHRCF
                     LDIADKIDTISFREELPDWRAEGTSDPREMILITQSLKELQLIMSDYVGIVRNDVRLQ
                     RAIRRLDLLWEETEVLYRTTTVSPQLLELRNMITVGYLIVKGASFRKESRGLHYNTDY
                     PAKSQLVQNIVL"
     gene            1215..2093
                     /locus_tag="PK977_03455"
     CDS             1215..2093
                     /locus_tag="PK977_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRF17192.1"
                     /translation="MYYVVYGFLWLISLLPLKVLYLFSDAVYGFLFYILKYRKEVVLK
                     NLAQAFPEKTEKERLIIARKFYHNLVDTFIETLKLFSSGKKFIERHNTADFSLIHRLH
                     GEGKAFQIHAGHQFNWEWINHHYAINFRQPLVAVYMPLANKTFEKIFYRMRTRYGTVM
                     LAATEMKTRFIPWRSREHALVLVADQNPGDPTNSYWFNFMGKPAPFIKGPERSARDKA
                     CPVVFAFARKTGRGKYNTEFIMAAENAATTSETELTKKYVAFLSAIIREQPDMWLWSH
                     RRWKWDWKPEYGEIIS"
     gene            complement(2116..4599)
                     /locus_tag="PK977_03460"
     CDS             complement(2116..4599)
                     /locus_tag="PK977_03460"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF17193.1"
                     /translation="MKIKTSLVFIILISFSLLSRAQKLPPREVPAKRTTQPVKIDGLL
                     NDSAWKDAAAMTDYVEFRPKIGAKEAFENRTITYLMYNDEGIYFGGYCYERTKDSIAT
                     ELAGRDGFGTNDYVGIIFDTYYDKINGFEYFVTPLNEQWDAKMAPSSNGNNEDFSWNG
                     VWQSGAVIHKDGWSFEMFIPYAAIRFGKKEVQNWGINITRRRRKTDQQYTWNPIDPNI
                     NGFLTQEGIWTGITNIKPPLRLQFSPYFSVYANHYPANQPGQKNWTSQINGGMDVKYG
                     INQAFTLDATLIPDFGQVQSDNQVLNLTPFEVKFNENRNFFTEGTELFSKGNLFYSRR
                     IGGTPIHLYDAYNVGANEKVIKNPNESKLINASKISGRTQKGLGIGVLNAVTKPQHAI
                     IENSQTKEQYKFETDPLTNYNILVLDQTMKYNSSVSLVNTNVWRSGSDYDANVTAALF
                     DLNNKKNMYNVGGKLAMSNLVGIQPDGKTLTGFSHELYFGKTSGKFNFNVSQQRTDTK
                     FNSNDLGYFTNNNFLDHSAWVGYRWTEPKKWYNRIFLNFNSYFSLLAKKFTGIDQNYQ
                     TAFFNINTNIQGKKLWWLGTFVGYGPGDNDFYEPRKEGMFFKRGSTAEAGGWFNSNES
                     KKYSFYIELFASKRFNFYNGYGFNISAGQNMRVNNKLSFSHSLNLNPQRNNMGFATID
                     NNGDVIFSRRDRNTVVNTLSTKYSFSNKMWLTFRARHYTSSVDIKELYNLQTDGKLLP
                     NTTFNANLNQNVNFFNIDMVYTWQFAPGSFINIVWKNAVFDFDNEVQSNYFKNFERTM
                     KASENNNISLKVIYFLDYLQLKNHKKKKG"
     gene            complement(4852..6120)
                     /locus_tag="PK977_03465"
     CDS             complement(4852..6120)
                     /locus_tag="PK977_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF17194.1"
                     /translation="MNFRQILLVMILVLTSCFLCFSLFGQPTTNGLLAYFPLNGNTNN
                     AGPANITATAVNTGYTTNNANLANNALQFAGNLNSYIDFVDNGNLDFSGTNNFTISFS
                     FFFNGSSTSGLLDNCLNYGGWGIWLWSTVAGTWNLQFNYKNNSVGSAAATAFTTGVWH
                     HVAAVRNNGTISLYIDGIFRLSSTEGTATPTYPLNMNAGAMTYGSFSPPRYNPFGGKI
                     DEIRIYNRALSAGEIGILVPFSLPLKLGEFTAVRKPTGIRLNWETLTEQNTSHFEIEK
                     STDGANWNSIGRVSAAGNSADKRSYSFTDNSPGTGVVFYRLKMVDNDSAFTYSRVITL
                     KNESQFTSLQLFPNPVTDNMQVQIPSSGKLDAVIWIIDAGGKTVHSRTLQLSEGQNTA
                     SIPVLHLTAGIYYLIIDDGKRKHTKSFIKN"
     assembly_gap    6212..6312
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6619..6628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6734..>7165)
                     /locus_tag="PK977_03470"
     CDS             complement(6734..>7165)
                     /locus_tag="PK977_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17195.1"
                     /translation="GGIHMDYTTNINSLKKVEIEETLPVNGNPQQAIKKTINAYQGSY
                     EEGIVELTLFCDYYKFWGQSNALVGLHFNPTVNYSEKIKKPVTNLYLGIIVPFIDKEK
                     QASKVSVEIFYSMQDMFNDLNNKPKSSSFGLRAALPITSIK"
     assembly_gap    7166..7189
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7190..>7526)
                     /locus_tag="PK977_03475"
     CDS             complement(<7190..>7526)
                     /locus_tag="PK977_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17196.1"
                     /translation="EIENHKLNIEILKEQMNKIIKDTAYFARETFKKRQLRDDKIIEL
                     TKKKKNIELLETDITWFSAGIRFKNNDIKLFAPLNLPATQYSDTSFVSRRVSLSISRF
                     RSTITQNRDF"
     assembly_gap    7527..7577
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7578..8164)
                     /locus_tag="PK977_03480"
     CDS             complement(<7578..8164)
                     /locus_tag="PK977_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17197.1"
                     /translation="MKKIFILSLLVYSSKYGFGQNLGKIYADPKQKIKIEEFKQAMNR
                     QFTKVITGTAFSNVGNFAAISTDAKSFSLAGNFVTERTVWGIELSGGATEGIFKLFNN
                     QILNSNFSGEIKYHRILKTGFASRNLYEVAEVESQLSEAYIQYSKDSLAIAQKRDLYK
                     TQIEIFNSESKISEGTKQKAKVDSLLSIVAISDKI"
     gene            8560..9126
                     /locus_tag="PK977_03485"
     CDS             8560..9126
                     /locus_tag="PK977_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17198.1"
                     /translation="MRKIILLVCCLVVFIGITFMSCQSNIKEADCKDLCHKLDSGYIN
                     YLKMVDSIGALRGEKNIGAAIPVSERIGNHFMGAYQNEFTRLNINQTHYLKLSRDNIA
                     FLYKHMIDANIDTLSFGFAKYDTTGFSIAGNLSDPVYNSRAEKMRNRNTIVIGEWGLL
                     ENGKKGPKPFSIKGIAFKFYDDWHNEDP"
     gene            9752..10582
                     /locus_tag="PK977_03490"
     CDS             9752..10582
                     /locus_tag="PK977_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF17199.1"
                     /translation="MLLQITEAGYYLIGATIIFILFFLIFFLFYRNVIRKRNEMFLNE
                     EKIKVNFRQTLLQSQLEIQEQTLKNISQEIHDNVGQVLSLAKLNLNTMDISKTEELQK
                     KIESALIQVSKAINDLRDLSKSFNTDNISALGLVRAVEYELDMIKKTGVHKTSFNVEG
                     KIKKLEPQTELILFRIVQETLHNIIKHAEASSIWISARYTENDLGLEIKDNGKGFHPT
                     AEGDQDTDSGLGIRNMHNRARLICAKFNLNSIKNEGTSVTIVLPFVSKQNSTEKNENL
                     "
     gene            10579..11217
                     /locus_tag="PK977_03495"
     CDS             10579..11217
                     /locus_tag="PK977_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17200.1"
                     /translation="MISVALIDDHVLLRNGLAGMLRESGYQVLFEADDGNEVASKVKA
                     SGPPDVALLDINMKHMNGYDTATWFKQNCPETKVLALSMYDDEENIIKMLKNGARGYI
                     LKDSHPTELKAAIEAVVTKGFYYSEMVTGRLMRSIIESGEEKDEQANLTGREIEFLKL
                     AASELTYKEIAEQMNLSHRTIDGYRDDLFEKLNIKSRVGLVLYAIRNGIVKV"
     gene            11310..12017
                     /locus_tag="PK977_03500"
     CDS             11310..12017
                     /locus_tag="PK977_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17201.1"
                     /translation="MSSHAPAIFWPHSEHWSVKIPVNTNHYEMPVLKEKGYAIMKTLD
                     ISHIPSSEWENLTYMDWKSGGDTNFAPLASCDGTIECKGFWENGKPDKGARFTSNVDL
                     CPNLMAYIKTIPANFGRVRIIKLEPQNHDQALRSVHRDDNNRLNPEGEGWVVRMWIEL
                     TDNPDSYMLLYDSDENNLPIKESEAHVPMPKGAHFVVDSQRLWHVGVHNGTKPRYAAI
                     ISCESSPELDAWIKSKM"
     gene            complement(12080..13489)
                     /locus_tag="PK977_03505"
     CDS             complement(12080..13489)
                     /locus_tag="PK977_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF17202.1"
                     /translation="MFIKQLYTGCLSEAAYYIESNGEAAVVDPLRDIDEYLQLANERK
                     VTIKYIFETHFHADFVSGHLDLSNATGAPIVYGPNTVTKFPVHVAKDGEKFSVGNLTI
                     EVLHTPGHTLESTCYLLKDEKGKDHCVFTGDTLFVGDVGRPDLAQKGAELTTNDLAGM
                     MYDSIHNKLFPLADEVIIYPAHGAGSSCGKNLGKETFSTIGEQKQYNYALQAATKEEF
                     ITAVTTGIAAPPQYFPVNARINKEGYDSINDVLSKGLTPLSVKAFKAELNEETIILDT
                     RNATVFTQGFVPGSISIGLEGRFAEWAGSILPFNTPMLLVTEPGKEKESIVRLARVGF
                     DKVKGYLDGGYDAWLKAGENIDMIIDVEADELMMDMPHDTNLVILDVRKVTEFGDGHL
                     KDAVNIPLDEMTDPGNIANLEDNMNLYIHCAGGYRSVIAASLLKRQGIHNLRNVLGGW
                     SKIKEQEKVEIIKEKSVLN"
     gene            complement(13565..13903)
                     /locus_tag="PK977_03510"
     CDS             complement(13565..13903)
                     /locus_tag="PK977_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF17203.1"
                     /translation="MNNYSLTINSVAMPGDTLKVDLLGVKKAALVLRAINHKLRQQIL
                     KHIDENGKITVTELYVALRLEQSVASQHLAILRKAGFVKTSRNGKFIYYSVNSNRIEE
                     LNQFVKSLVG"
     gene            complement(14014..14691)
                     /gene="tsaB"
                     /locus_tag="PK977_03515"
     CDS             complement(14014..14691)
                     /gene="tsaB"
                     /locus_tag="PK977_03515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRF17204.1"
                     /translation="MSLLLNIDTALETGSVCLANSATIIGSSVNHQQHDHAAWLHPAI
                     HELLQNNNLEAGQLDAIAVTIGPGSYTGLRIGLSAAKGLCYALSKPLITVSTLEVLAS
                     LAVDEADQLICPVIDARRMEIFTALYDKDLQLIRKPVALVLQHDSFNDILSDQQMLFC
                     GNAVTKMQPLITSPNASFTNKASTALHLAPLAYQRFTSGLFADLAYTEPLYLKEFYFP
                     QPKVKGN"
     gene            complement(14688..15317)
                     /locus_tag="PK977_03520"
     CDS             complement(14688..15317)
                     /locus_tag="PK977_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRF17205.1"
                     /translation="MKIAVIAGENEKAEFLENGINTSNEVLWLNSPESVPADCNCCID
                     LLFNNESSRVQLLSSLTIPLIVVNAVAITSAGLPGNFIRINGWSTFIKRHTIEACCLQ
                     ESMKGKVEEVFACFNKKINWTPDIPGFVAARIISSIINEAYLALGEKVSTKAEIDTAM
                     KLGTNYPYGPFEWSSKIGLTYVYQLLKAMAEENERYKPADELTKEVSAL"
     gene            15382..15762
                     /locus_tag="PK977_03525"
     CDS             15382..15762
                     /locus_tag="PK977_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF17206.1"
                     /translation="MSKTVIKTPDAPAPIGPYNQAILSGNMLFISGQICIDPSTGELK
                     NKDVQEETHQVMHNLKAILQQAVITFSNVVKTTIFITDMHQFSVINEVYGKYFEGDFP
                     ARETVQVSALPKFVNVEISMIAVK"
     assembly_gap    15891..15904
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15905..>17663)
                     /locus_tag="PK977_03530"
     CDS             complement(<15905..>17663)
                     /locus_tag="PK977_03530"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF17207.1"
                     /translation="GNDYRSASGEPGPKYWQNRADYKINATLDTGTHRITGDVEITYT
                     NNSPDNLRFLWLQLDQNIYKKDSRGSATTTEAGGRWANAKFTDGYSIKSVALESNGKK
                     ITPQYNISDTRMQVWLQEAVKSSGGKTKLFIQFEFTVPEYGTDRMGRLKTKNGIVYEV
                     AQWFPRMCVYDDIQGWNTMPYIGAGEFYLEYGDIEFTVTAPSSLIIVGSGELLNPQEC
                     YTPEQMKRWETAKNSDKTVAIRSDKEVNDPTSRPRQANCTWKFRITNTRDVAWAASKA
                     FVQDAAKINLPSGKKSLAISVYPVESIKKDGWQRSTEMVKGSIEHYSKKWFEYPYPAA
                     TNVAGIVGGMEYPGIVFCSHADSGTGLWGVTDHEFGHTWFPMIVGSNERRYAWMDEGF
                     NTFINGLSTDAFNNGEFKETSFFGDPNSSFMIKYTFGDEMDGLYTYPDVIQQDNLGIA
                     AYMKPAQMLTALREVVLGKDRFDAAFREYINRWAFKHPTPWDFFHTMENVAGEDLSWF
                     WRGWVLNTWKLDQKVKEVKYIKENPANGADITLENAEKMVMPVTVLIKETNGKEHRID
                     LPVEVWQRGAEWRFSVPTTS"
BASE COUNT         5054 a         3294 c         3493 g         5540 t
ORIGIN      
        1 tgatcgatgc tgtagggaaa cataaaaata ttgaatttat caaacattgt tttgtagtag
       61 atattattac gcagcatcac ctcggctatc taattacaaa atctactcct gatattgaat
      121 gctacggagt gtatgtgctg aatctggaga caaataagat tgaaaaaatt ctggctaaaa
      181 caacattgct cgctacaggg ggaaacgggc aggtgtacag aaccactact aatcctttca
      241 ttgcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnncaa ccgctttgta tgaaccgggt gtcggcgggc
      361 aggccttttt aattacagaa gctgtaagag gggatggggg aattcttcgg aataaaaagg
      421 gtgaggcatt catggagaag tatgacccga gaaaggatct ggcgccaaga gatattgtag
      481 caagggcaat tgacagtgaa atgaaaaaga ccggtaccga acatgtgtgg ctggattgcc
      541 ggcattttga taaagaaaag tttattgaac atttccccaa tatctaccag aaatgtatgt
      601 cgataggtat tgatataacc caacacatga ttcctgttgc tcctgctgca cattatagtt
      661 gtgggggaat aaagacagat gaatggggaa gaagctcgat taaaaatctg tatgcctgcg
      721 gcgaatgtgc cagtaccggc ctgcatggag ctaaccggct tgccagtaat tcactgctgg
      781 aagcaatggt ttttgcccat cggtgttttt tagatatcgc tgataagatt gatacaattt
      841 cattcaggga agaattgccg gattggcggg ccgagggaac ttcagacccc agggagatga
      901 tccttataac gcaaagtctg aaagaacttc agctgatcat gagtgattat gtaggtattg
      961 taagaaacga tgttcgttta caaagagcca tcaggagatt agacctcctg tgggaggaaa
     1021 ctgaagtact gtacagaaca acaacggttt cacctcagtt gcttgaattg cgtaatatga
     1081 taacagtagg ttacctgatt gttaaagggg catcattccg aaaagaaagc cgtggcttgc
     1141 attataatac agattatcct gccaagagcc aactggttca gaatatcgtg ttgtagtggc
     1201 tatctttgcg ggctatgtat tatgttgttt atggattttt gtggttgatc tctttattac
     1261 cattaaaggt gctgtatctt ttttcggatg cagtttatgg attccttttt tatattttga
     1321 aatacaggaa ggaagtagtg ctgaagaacc tggcacaggc tttcccggag aaaacagaaa
     1381 aagaacgatt gattatcgcc aggaaattct atcacaacct tgttgatacg tttattgaaa
     1441 cactgaaatt gttctcttcc ggtaaaaagt ttatcgaaag acacaatact gctgattttt
     1501 cactgattca ccgcttacat ggtgagggta aagcttttca aattcatgca ggccatcaat
     1561 tcaattggga gtggatcaat catcattatg ctatcaattt ccggcaacca ttagttgctg
     1621 tatatatgcc tcttgccaat aaaacatttg agaagatctt ttacaggatg cggacaagat
     1681 atggcactgt tatgctagcg gctacagaga tgaaaacaag gtttataccc tggcgcagca
     1741 gggaacatgc attggtattg gtggcagatc aaaacccggg agaccccact aattcttatt
     1801 ggttcaattt tatgggcaaa cctgctcctt ttataaaagg tccagaaaga tcggcaagag
     1861 ataaggcctg cccggtcgtt tttgcttttg cccggaaaac aggcaggggc aaatacaata
     1921 cagaatttat aatggcagca gaaaatgcag caacaacatc cgaaacagaa ttgacaaaaa
     1981 agtacgttgc ctttctatca gctattatcc gggaacagcc tgatatgtgg ctctggagtc
     2041 accggcgctg gaaatgggac tggaagccgg agtatggaga aatcatttca taaaaaagcc
     2101 ctattgcggg gctttttatc ctttcttctt tttgtgattt tttaattgca ggtaatcgag
     2161 gaagtaaata acctttaaag aaatattgtt attctcactg gctttcatcg tcctttcaaa
     2221 atttttgaag tagtttgatt gtacttcatt gtcgaaatca aacactgcat ttttccaaac
     2281 aatattgatg aagctgccgg gtgcaaactg ccaggtataa accatatcaa tattgaagaa
     2341 gtttacgttc tggttaaggt tggcattaaa ggttgtgttg ggtagcaatt tgccgtcagt
     2401 ttgaagattg tacagctctt ttatgtcaac agaactggta taatgcctgg cacggaaggt
     2461 aagccacatc ttattggaga aactatattt tgtacttaat gtatttacaa ccgtattcct
     2521 gtcacggcgg gaaaaaataa catcaccatt attatcaata gtggcaaagc ccatattatt
     2581 gcgttgtgga ttcaggttca gtgaatgtga gaaggataat ttgttattga ccctcatatt
     2641 ttgccctgcg ctgatattga acccgtagcc attgtaaaaa ttgaagcgtt tgctggcgaa
     2701 caattctata taaaacgaat atttcttaga ctcattactg ttgaaccatc cgccggcttc
     2761 agctgttgag cctcttttga aaaacatacc ttcttttctt ggttcataaa aatcattgtc
     2821 gccgggtcca tagccgacaa aagttcccag ccaccatagt ttcttgccct ggatatttgt
     2881 attgatatta aagaaggcag tttggtaatt ctgatcgata ccggtgaatt tctttgccag
     2941 taaactgaag taggaattga aatttaaaaa tatccggtta taccactttt taggttctgt
     3001 ccagcgatac cctacccatg cactgtggtc taagaaatta ttattggtaa aatatccgag
     3061 gtcattgctg ttgaattttg tatccgttct ttgctgcgat acattaaagt tgaattttcc
     3121 actggttttt ccgaaatata attcatggct gaagccggtt agtgtcttgc catcgggctg
     3181 aataccaacg aggttgctca tcgccagttt accacctaca ttgtacatat tctttttgtt
     3241 gttcaaatca aaaagagctg cggtaacatt ggcgtcatag tcggagccgc ttcgccatac
     3301 attggtattc accagcgaga cactggaatt gtatttcatg gtctggtcaa gcacaaggat
     3361 attgtaattg gtaagcggat cagtttcaaa tttatattgc tcttttgttt gtgaattttc
     3421 aataatagca tgctgaggtt tggtaacggc attcaaaacg ccaatgccca gtcctttttg
     3481 tgttcggccg gatatcttag atgcattgat aagttttgat tcattgggat ttttgattac
     3541 tttttcatta gccccaacat tataagcgtc gtatagatga atcggtgtac caccaattct
     3601 tctggagtag aaaagatttc ccttgctgaa taattcagtt ccttctgtaa aaaaattcct
     3661 gttttcattg aattttactt caaagggtgt aaggttcaaa acctggttat cactttgtac
     3721 ctggccaaaa tcaggtatca aagtagcgtc taaggtaaaa gcctggttaa tgccatattt
     3781 cacatccatg ccaccattaa tttgactagt ccagtttttt tgaccgggct gattagcagg
     3841 atagtgattg gcatatacag agaaataggg ggagaattgt aaacgtaacg gcggctttat
     3901 attagtgatg ccagtccaga ttccttcctg tgtaagaaaa ccatttatat tcggatcaat
     3961 cgggttccag gtgtattgct gatccgtctt tcttcttctt cgggtaatat ttattcccca
     4021 gttctgaact tcttttttac caaaacgaat ggctgcataa gggataaaca tttcaaaact
     4081 ccagccatct ttatgtatta cagcgccgct ttgccaaact ccattccaac tgaagtcctc
     4141 attgtttccg ttgctactcg gtgccatttt tgcatcccac tgttcattta acggtgtaac
     4201 aaagtattca aacccgttta tcttgtcata atatgtatcg aatattatac ccacataatc
     4261 attagtgcca aagccatctc tgccggccag ctctgtagca atactatctt tggtgcgttc
     4321 gtagcaatac cctccaaagt agatgccttc atcgttgtac atcaggtaag tgatcgttct
     4381 gttttcaaaa gcttctttag cgccgatttt tggtctgaat tctacataat ctgtcattgc
     4441 agcggcatct ttccaggcag aatcgttcag aagtccgtca atctttacag gctgtgtggt
     4501 acgtttggca ggtacttcac ggggaggcaa tttttgtgcc ctgctgagca gcgaaaatga
     4561 gataagaata ataaagacca ggctggtttt gattttcatg gctatactta tagttggttt
     4621 gacgaagacc actccttttg agttacatca catatttatg tgatatacta acctcttcgt
     4681 aggttgaaga taatggcgaa tgaggcggta attattggat ttaggtgaat ggtttattct
     4741 gcctgacgaa tggcaaaaaa taaagggtgt taacttttta ttttgaaaag agaaagagct
     4801 gattattaat aatcagctct tttcaaatgc atcacaggtt aaggaggatg cttagttttt
     4861 gatgaaggat tttgtatgtt ttcttttacc atcatcaatg ataaggtaat agataccagc
     4921 cgttaaatga agtactggta tacttgcagt attttgtcct tctgaaagtt gtagtgttcg
     4981 tgaatgaact gtttttcctc ctgcatcgat aatccaaatt acggcatcca attttccgga
     5041 tgatggtatt tggacctgca tgttatctgt tacaggattg ggaaacagtt gcagtgaagt
     5101 aaactggctt tcatttttca aagtgatgac acggctgtag gtaaaggctg aatcattatc
     5161 taccatcttc aaccggtaaa atacaacacc cgtgcctggt gaattatccg taaaactgta
     5221 cgatctttta tcagcagaat taccagcagc agatacacgg cctatgctgt tccagttggc
     5281 cccatcggta cttttttcta tttcaaaatg agaagtattt tgctcagtaa gtgtttccca
     5341 gtttaaccgg atgcctgttg gttttcttac tgctgtaaat tctcccagtt ttagcggtaa
     5401 cgaaaatggc acaagtattc caatttctcc cgctgataat gcccggttat aaatccttat
     5461 ttcatctatt ttgccaccga aggggttata cctgggagga ctgaagcttc cataggtcat
     5521 agctcctgca ttcatattta aaggataagt tggcgtagca gttccttcag tggatgatag
     5581 cctgaaaata ccatctatat agagcgaaat cgtaccattg ttacgtacag cggccacatg
     5641 gtgccataca ccagtagtaa aggcagtggc agcagcgctg ccgactgaat tatttttata
     5701 gttaaactga aggttccagg tgccagcgac agtagaccaa agccaaatac cccagccacc
     5761 atagttaagg caattgtcta agagacctga ggtcgaacta ccattaaaga aaaaagaaaa
     5821 cgagatagtg aaattgttgg tgcctgaaaa atccaggttg ccattatcta caaagtctat
     5881 atacgagtta agattacctg caaattgtaa agcattgttg gccaaattgg cgttattggt
     5941 agtatagcca gtattaacgg cagtggcagt aatattagcc gggccggcat tattggtatt
     6001 tccgttcagt ggaaaataag caagcaagcc gtttgtagtg ggttgtccaa aaagagagaa
     6061 gcaaagaaaa caactggtaa gtaccagtat cattactaga agaatttgtc tgaagttcat
     6121 aacctgtgat ttttagtgta aacaataatg gctttcacag gtgcagatta ttgccgtaaa
     6181 aatggttgct gtagcagtaa cagaaaagag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nngatattga aaagaggaaa agctgaacct aatcatttct gctttttccg
     6361 tgggaagttg ttcgatagga gattcggtga tgagggacat aaggctttat gtaaaaacca
     6421 ttaagataac aagaaaacag atagtttact atatcagcgc tttcttttcc ttatataaca
     6481 atggcgagtt ttctccgtta atttcattct ccttgcatat tataatgcct gctccgatct
     6541 gacttgtagg gcttctatag gcacaaatac ttctaagatt gccatttgat gcggtccggg
     6601 attttgattg ctgtagttnn nnnnnnnnaa aagaaattat tcattgcaca ttattaatta
     6661 ttaattcggt tacaaataat ctgcttctcc atccatggga tctgcaaatc gcctgatagt
     6721 aaatcataat ttattatttt attgatgtta ttggcaatgc tgcccttaaa ccaaatgaac
     6781 ttgactttgg tttattgttt aaatcattaa acatatcctg catgctataa aatatttcta
     6841 cactaacttt tgaagcttgt ttttctttgt caatgaaggg aacaattatt cctaaatata
     6901 aatttgtaac tggttttttt attttttctg agtaattcac cgttgggtta aagtgtagcc
     6961 caactagtgc gttagattgc ccccaaaatt tgtagtagtc gcaaaacagt gtgagctcaa
     7021 ctattccttc ttcatagctt ccctggtagg cattaattgt ttttttaatg gcttgttgtg
     7081 ggttgccgtt tacaggcaaa gtttcttcaa tctcaacctt ttttagggaa ttaatgtttg
     7141 tagtataatc catgtgaatt cctccnnnnn nnnnnnnnnn nnnnnnnnna gaaatctctg
     7201 ttttgtgtta tagtgctacg gaatctcgaa attgaaaggg aaactctacg gctaacaaaa
     7261 cttgtatcac tatattgtgt tgccggtaaa ttaagcggag caaaaagttt aatatcgtta
     7321 ttcttaaacc gaattccagc tgaaaaccag gttatatctg tttctaagag ttcaatattc
     7381 ttttttttct tggttaattc tattatttta tcatctctaa gttggcgttt tttgaaggtt
     7441 tctcttgcga agtatgctgt gtccttaatt attttattca tttgttcttt tagtatctca
     7501 atatttaatt tgtgattttc aatttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnttt attttatctg atattgctac aatcgataat agggaatcaa
     7621 ctttggcttt ttgctttgtt ccttcggaga ttttagattc actattaaat atttcaatct
     7681 gcgttttata aaggtctctt ttttgtgcta ttgccaagga atctttgcta tattgaatat
     7741 atgcctctga aagttggctt tctacttcag caacttcgta taaatttctt gaagcaaacc
     7801 ctgtttttaa aattctatgg tatttaattt cgcctgaaaa atttgagtta agtatttggt
     7861 tgttgaatag cttaaatatt ccttctgttg cgccacctga taattcaatt ccccatactg
     7921 ttctttccgt tacaaaattt cctgcaagag aaaatgattt ggcatctgtg ctaattgcag
     7981 caaaatttcc tacattacta aatgcagttc cggttataac tttggtaaat tgcctgttca
     8041 tagcttgttt aaactcttct attttaattt tttgcttcgg gtcagcataa attttaccca
     8101 aattttgacc gaatccatat ttagaactat agactaataa tgacaggata aatatttttt
     8161 tcatattcgt tttttagagt tttatatgat ggaaagttga gtacagcatt ttgatatatt
     8221 catgttgtta taatataaag gtattaagac aaaaaaaaag acaaaccgcc tttacaccca
     8281 attaaccccg ttttttcacc ctaccagcag ccgaccactc ataagccggg gtattaaact
     8341 atctcgaaat ctgaggagag tttacatttg taattttctt tagcctctga taaaacagtt
     8401 tcttttagat ttcgggcatg aaatgattga agcctagaag ccaacgtagg aaggggtgaa
     8461 aaaacggtat agtatcggtt aaaaaactat tggtatttag acttggagat accagatttg
     8521 tgcggtattt taatttattc ttttacctta aataatttta tgagaaaaat tattttgctc
     8581 gtatgttgtc ttgttgtttt cattggtatc acttttatgt cctgtcaaag caatatcaaa
     8641 gaggcagact gcaaagactt atgtcataaa ttagattccg gttatattaa ttacctgaaa
     8701 atggttgatt caattggcgc tttacggggt gagaaaaaca tcggtgccgc catacctgtt
     8761 tcggagcgaa ttggtaatca tttcatgggg gcatatcaaa atgagtttac aaggttgaat
     8821 ataaatcaaa cgcattactt gaaattgagc cgggataata ttgctttttt atataagcat
     8881 atgatagacg caaacattga cactctttcc tttggctttg ctaaatacga tacaacagga
     8941 ttttctattg ccggaaattt gagtgatccg gtatataaca gccgtgctga gaaaatgaga
     9001 aacaggaata caattgtgat tggtgagtgg ggattattgg aaaatggaaa gaaaggtcca
     9061 aagcctttta gcattaaagg aatagcattt aagttttatg atgactggca caatgaagat
     9121 ccataagtaa acagatgatt actttcttaa taaactattc tcttgaatta tcggcatttc
     9181 tcaccgggct gatgctatat cgtaagcttc agcccggtat attcaagctg cttgtattac
     9241 tacttttttt tactgttgta aatgaagggt tgtcttatct gggtttttat acacagatac
     9301 atgtaaaaaa gactttgata tatgccatct tttttattat agagtgtatc atcttctgga
     9361 ggctatttgc tgtaaaacaa aaagcaggta agcgatggtt ttatcattcg atattcttgg
     9421 cagcactgat actgcaagtg ttttttcttt tccgttacgg atataaacaa atgaattcac
     9481 cgtttctaaa tgttatctgc tgttatacta tttttttagg catcagttat tactacaaat
     9541 tgtatacaga acagaaatat tttgatataa caaaggatcc tttcttctgg cttgcaactg
     9601 gtataataat cgtaaatttt gtacacctgt tttatgtaaa tgctattttt ctaaatgttt
     9661 tccggaatga tccgaaccgg tttaagatat ttaaaaattt aaacacaatc gggaatgtta
     9721 tatattatcc attattaata aaagctttgt tatgctcctc caaattacag aagcaggcta
     9781 ctatctgata ggggctacaa taatatttat cctgtttttt ttaatattct ttctatttta
     9841 tagaaatgtg atacgtaagc gaaacgaaat gttcttaaat gaggagaaaa taaaggtaaa
     9901 tttccggcag accttacttc agtcgcaact cgaaatccag gagcaaacct taaaaaatat
     9961 ttcgcaggaa atacatgata acgtaggaca ggtgctaagc cttgctaaac ttaacctgaa
    10021 tacgatggat atttccaaaa cagaggaatt gcagaaaaaa attgaaagtg cccttatcca
    10081 ggttagcaaa gccatcaacg acctacggga tctgtcgaaa agttttaata cggataatat
    10141 atccgcttta ggactggtaa gggcggtaga gtatgagctc gatatgatta aaaagaccgg
    10201 tgtacataag acaagcttca atgtagaggg aaaaataaaa aaacttgaac cccaaacgga
    10261 acttatactt tttcgtatcg ttcaggaaac cctccataat attatcaagc atgccgaggc
    10321 ttcatctata tggatttcgg cccgctatac agaaaatgat ttagggctgg aaataaagga
    10381 taatgggaaa ggctttcatc ctactgctga aggcgatcag gataccgatt cgggacttgg
    10441 tatccgtaat atgcataaca gggcccggct gatctgtgct aaatttaacc tgaatagtat
    10501 taaaaatgaa ggaacatccg ttacaattgt cttgcccttt gttagcaaac aaaattctac
    10561 agaaaaaaat gagaacttat gataagcgtt gcccttattg atgatcatgt attactgcgc
    10621 aatgggctgg ccggtatgct aagagaatct ggttaccagg tattattcga agcagatgac
    10681 ggcaatgaag tagcatctaa agtgaaggct tccggtccgc ctgatgtagc gcttcttgac
    10741 attaatatga aacacatgaa tgggtatgat acggcaacat ggtttaagca gaattgtcct
    10801 gaaacaaaag tactggcatt aagcatgtat gatgatgaag agaatattat aaaaatgctg
    10861 aagaacggtg ccaggggata tattctcaag gattcacatc caactgaact gaaagcagct
    10921 atcgaggcag tagtaacaaa gggtttttat tattccgaga tggtaaccgg ccggttaatg
    10981 cgaagtatta ttgaaagtgg agaagaaaaa gacgagcagg ctaacctgac agggagagaa
    11041 attgagtttt tgaaattggc tgcaagtgaa cttacctata aagaaatagc cgaacaaatg
    11101 aacctgagtc accgcaccat tgacggctac cgtgatgacc ttttcgaaaa gctaaatatc
    11161 aaaagccggg taggcctggt gttatatgct atccggaatg gaatcgttaa agtttgataa
    11221 ttgtggcaag atcggtaaat gtcaattcat ggtttgccta tatttcctat tttagcaact
    11281 tacgaaatca acaaccttaa tcttttttta tgtcatcaca tgcacctgcc atattctggc
    11341 cccacagcga acactggtct gtaaaaattc cggtcaatac caatcattat gaaatgcctg
    11401 ttttgaaaga aaaaggctac gccattatga agacgctgga tatatctcat atcccttctt
    11461 cggagtggga gaatttaacc tatatggact ggaaaagtgg tggtgatacc aattttgcgc
    11521 cgctggcttc ctgcgatggt accattgagt gtaaaggatt ttgggaaaat ggaaaaccgg
    11581 ataaaggggc gaggtttacc tcaaatgtgg atttgtgccc caacctgatg gcttatatca
    11641 aaacaattcc tgccaatttt gggagggttc gtatcatcaa actggagcca cagaatcatg
    11701 atcaggcact gcgttctgtt caccgggatg ataataaccg cctgaacccg gaaggagagg
    11761 gctgggtagt gagaatgtgg atcgagctga ctgataatcc agacagctat atgctgctgt
    11821 acgatagtga tgaaaataat ttacccatca aagaaagtga agcccatgta ccgatgccga
    11881 agggtgccca ttttgtagtt gactcacaaa gattgtggca tgtgggtgtg cataatggaa
    11941 caaaaccaag atatgcggcg attattagtt gtgaaagctc acctgaactg gatgcatgga
    12001 taaagagtaa aatgtgacta ctgatttaac ggattaaaat aatttacaaa aaaagagccc
    12061 tcaaccgaga gctctttttt tagttcaaca cactcttttc ttttataatc tctacttttt
    12121 cctgttcctt tattttactc catccgccca gtacatttct caagttgtgt atgccctggc
    12181 gttttaaaag tgaagctgca attacactgc gataaccacc ggcacaatga atataaaggt
    12241 tcatgttgtc ttcaagattt gcaatgttac cgggatcagt catttcatct aacggaatat
    12301 tgacggcatc ttttagatgg ccatccccaa attctgtcac ttttcttaca tcaagtatca
    12361 caaggttagt atcgtgtggc atatccatca tcagctcatc tgcttccaca tcaatgatca
    12421 tatcaatatt ttctcctgcc tttagccatg catcgtagcc gccatccagg taacctttta
    12481 ctttatcaaa acccactctt gccaacctga caatcgattc tttttcttta cccggttctg
    12541 ttaccagcag cataggtgta ttaaacggta aaatgcttcc tgcccattcg gcaaatcttc
    12601 cctccaggcc aatactgatg gagcctggca caaatccttg ggtgaaaaca gtagcgttcc
    12661 ttgtatcaag aataattgtt tcttcattca gttcggcttt aaatgccttc actgataatg
    12721 gcgtaagacc ttttgacagt acatcattaa tgctatcata cccttctttg tttatccggg
    12781 catttacagg aaaatattgt ggcggagcag caatacctgt agtaacggct gttatgaatt
    12841 cttcttttgt tgccgcctgc agggcatagt tatattgctt ttgctctcct atagtactaa
    12901 atgtttcttt gcccagattc ttaccacaac tgctgccagc tccatgtgct ggataaataa
    12961 ttacttcatc agcgagagga aataatttgt tatgaatgct gtcgtacatc atgcccgcaa
    13021 gatcgtttgt agtgagttca gctccttttt gtgccaggtc gggacggcca acatcgccaa
    13081 caaataatgt atctccggtg aacacacaat gatctttacc cttttcatcc ttcaaaagat
    13141 agcaggtgga ttccaacgta tggccgggag tatgtaatac ttcaattgtc agatttccca
    13201 cactaaattt ttctccgtct tttgccacat gaacgggaaa ctttgtaaca gtattaggtc
    13261 catacacaat cggtgcaccg gttgcgttac tgagatcaag gtggccactt acaaagtcag
    13321 catggaaatg tgtttcgaaa atatatttaa tggtgacttt cctttcattg gccagttgca
    13381 ggtattcgtc aatgtcacgt aggggatcaa caacagctgc ctcgccgttg ctttcaatat
    13441 aataggcagc ctcactcagg cagcctgtgt atagttgctt aatgaacata ggtgaaattt
    13501 tgaacagcaa atttaccggc taaagcggta tgaattcaat ttgtgtttta tgacaatggg
    13561 aaatttatcc taccaaactc tttacaaact ggttcaactc ttctatacgg ttggaattca
    13621 cggaataata gatgaattta ccattgcggc tggtcttaac aaatccggct tttctaagta
    13681 tagccaggtg ctgagacgca actgattgtt ctaatcttaa agcgacgtat aactcagtaa
    13741 cagttatttt tccgttttca tcaatatgct ttaatatctg ttgacggagt ttatgattaa
    13801 tagcccttaa aacgagtgca gcttttttta cacccagcag atccacttta agagtatcgc
    13861 ctggcattgc tacagaattg atcgtaagtg agtagttgtt catcaatagt ttctttgagg
    13921 tagtacaaaa atatcgttgg ctttcaggta agagaaatta ctcctgttaa atgtgattaa
    13981 cggcgaaaag aacttaccga ctgataagac gaattaattc ccttttactt tcggttgcgg
    14041 aaaataaaat tccttgagat ataatggctc tgtataggca aggtcagcaa ataagcctga
    14101 agtaaaacgc tgataagcaa gcggggcgag atgaagggct gtggaagctt tgtttgtaaa
    14161 agaagcattg ggtgaggtaa taagcggttg catttttgtc acagcattac cacaaaaaag
    14221 catctgctga tctgaaagaa tatcgttgaa gctgtcatgt tgcaaaacca aagcgacggg
    14281 tttacggata agctgaagat ctttatcgta aagtgctgta aatatctcca tcctccgggc
    14341 atcgataaca ggacaaataa gttgatcggc ttcatcaaca gctaacgagg ccagtacttc
    14401 cagtgtgctt acagttatta aaggtttact tagtgcataa caaagacctt ttgctgcaga
    14461 gaggcctatc ctgagaccgg tgtatgaacc gggaccgatg gttacggcta ttgcatctaa
    14521 ttgtccggct tccaaattat tattttgtaa aagctcatga atggcagggt gcagccatgc
    14581 agcatggtca tgctgctgat ggtttacact gcttccaatt attgttgctg aattagcaag
    14641 gcaaacagat cctgtttcaa gggcggtatc tatgtttaat aacaggctca taaagcacta
    14701 acttcttttg ttaactcatc agcaggttta tagcgttcat tttcttcagc catggctttc
    14761 aataactggt aaacataagt aagtccaatt ttgctactcc attcaaacgg gccataggga
    14821 taattggtac ctaatttcat agccgtatct atttcagctt ttgtactcac cttttcaccc
    14881 aatgcaagat aagcttcatt gataattgaa ctaatgatgc gggcagccac aaaaccaggt
    14941 atatcaggag tccagttaat ttttttatta aaacaggcaa atacctcttc aacttttcct
    15001 ttcatcgatt cctgtaagca gcaagcttca attgtatgcc gttttataaa agtagaccat
    15061 ccgtttatgc ggataaagtt gcctggcaaa ccggctgatg taattgctac tgcattcact
    15121 acaatcaacg gtatggtaag ggatgataat agttgtaccc tgctggattc gttattgaac
    15181 aagagatcaa tacagcagtt gcagtcagcc ggcacacttt ccggggagtt aagccataat
    15241 acttcattac ttgtattaat cccattttcc agaaattctg ctttttcatt ctctcctgca
    15301 atgactgcaa tcttcataat ctattttttg caaagaaaac acataaacct aaattcgtta
    15361 ttcctaattt gatttaaact tatgagtaag accgttatta aaacccctga tgccccggcg
    15421 cctatcggac cttataacca ggctattctg tccggtaata tgcttttcat ttccgggcaa
    15481 atctgtattg atccttcaac cggggagctg aaaaataaag atgtacagga agaaacacac
    15541 caggtgatgc ataacctgaa agctattttg cagcaggcag tgattacttt cagtaatgtt
    15601 gtgaagacca ctatttttat cactgatatg caccagttta gtgttatcaa tgaagtgtat
    15661 gggaaatatt ttgaaggaga ttttcctgcc agggaaacag tacaggtttc tgcattgccc
    15721 aaatttgtta atgtagaaat tagtatgatt gctgttaaat aaactgaaac tatctcttcg
    15781 aacatattta aaaaccctcc tttcggaggg tttgttttaa aaagcccttt tcttccagga
    15841 attattttcc cggttccagt ccggtaattt tttgtcggga tccaatatca nnnnnnnnnn
    15901 nnnncactgg tagtgggaac actaaaccgc cattctgccc ctctttgcca aacttctacc
    15961 ggcagatcaa tacgatgttc ttttccgttt gtctctttta ttaataccgt aacgggcatt
    16021 accatctttt ccgcattttc caaagtaata tcagcgccat tagccggatt ttccttgata
    16081 tactttactt cttttacttt ttggtcgagc ttccaggtgt tgagcaccca gcctctccag
    16141 aaccagctaa ggtcttctcc ggccacattt tccatggtgt gaaagaaatc ccatggtgtc
    16201 ggatgtttaa aagcccaccg gttgatatat tccctgaacg ctgcatcaaa acgatctttg
    16261 cctaatacta cttctctcaa tgctgtaagc atttgtgcgg gtttcatata ggcagcaatg
    16321 cccagattat cctgttgtat tacatccgga taagtgtaga ggccatccat ctcatctcca
    16381 aaagtatatt tgatcatgaa agatgaattg ggatcaccga agaaactggt ttctttgaat
    16441 tcaccgttat tgaaagcatc ggttgaaaga ccattaatga atgtattgaa tccttcatcc
    16501 atccaggcat atcttctttc attgctgcca acgatcatag ggaaccaggt atggccaaac
    16561 tcatgatcgg ttacacccca taaaccagtt cctgaatcgg catggctaca aaacacaata
    16621 ccgggatatt ccataccgcc tactataccg gctacgttgg tggctgccgg gtaaggatat
    16681 tcaaaccatt ttttggaata atgttcaatg gaacctttta ccatttctgt cgaacgctgc
    16741 cacccgtctt ttttaatact ttcaacaggg taaacactaa tagctaatga ttttttacca
    16801 ctgggtaaat taatttttgc ggcatcttga acaaatgcct tgctggccgc ccatgccaca
    16861 tctcttgtat tggtgatcct gaatttccat gtgcaattgg cttgcctggg tcttgatgtt
    16921 gggtcattca cttctttgtc actgcggata gcaactgttt tgtcgctgtt tttggcagtt
    16981 tcccatctct tcatttgttc tggtgtataa cattcctgcg ggtttaataa ttcacccgag
    17041 ccaacaataa tcaggctgga aggagcagta acggtaaatt ctatatctcc gtattccaaa
    17101 taaaactcac cggctccgat ataaggcatg gtattccatc cctgaatatc atcatataca
    17161 cacatacggg gaaaccattg tgccacttcg tatacaatcc cattttttgt ttttaatctt
    17221 cccatcctat cagttccata ttccggtaca gtaaattcaa actgaataaa gagttttgtt
    17281 tttccgccag atgattttac agcttcctgc aaccaaacct gcattcttgt atctgaaatg
    17341 ttgtattgtg gagtgatttt ttttccatta gattcaagag ctactgattt aatgctgtag
    17401 ccatcggtaa actttgcatt ggcccagcga ccacctgctt ctgtagtagt ggcggaaccg
    17461 cgggaatctt ttttataaat attctgatct agttgcagcc agagaaaacg caggttatcc
    17521 gggctgttgt tagtataagt aatttctaca tcaccggtga tgcggtgggt gccggtatct
    17581 aaagtagcat tgatcttata atctgccctg ttttgccagt atttgggtcc gggttcgccg
    17641 ctggcactgc ggtaatcgtt gcc
//
LOCUS       DAORLS010000025        17528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1734_length_17528_cov_2.090711, whole genome shotgun sequence.
ACCESSION   DAORLS010000025 DAORLS010000000
VERSION     DAORLS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17528
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_17528_cov_2.090711"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2855
                     /locus_tag="PK977_03535"
     CDS             <1..>2855
                     /locus_tag="PK977_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF17208.1"
                     /translation="ILKVWDLATGRTISSFTAHQLEINSVQYSPNGRYIATSSEDSTI
                     KVWNAYDFSLLYTIKRPKPESDVIFSPDSKFLVAGGRDSLIKFISIHSGKVLKTLKKH
                     RGTVTSLCFMPSDNDYLFSGGADSVVYKWNLTTNDWDHWYGAKGRVMDVSVSNDGKYL
                     SFVSSDTLIRVWKLENNKFYFNIRPNYSTTDPVDIAFPSFSPDSRYMALALKNDTLDI
                     IELSTLKEQVYGFKMEKFQGLYDMVFSPDGASLASRMSLGGPLRVFNFSGWDFHSNAT
                     VNSKDLKNYSNLPLSVQFTKDDNELVIVHDAVSKIDLRNGATTHLYNESLFFLNNNIL
                     LNDGKTGLYNDLVRPGLKFYDIPSKQVKLSLSLPDKSEELRRFELSADNSLIFIGGIN
                     NSIAGFSLPGGGQLFSAKYNLGDRESLIFLRYDSIRNRLYAIGKNQEVLIIHPQTGDS
                     IASIKAAFPQTIEVTPKYLFVTCDSSLVYKYDANNLRLLKKIPVHKSKSNCYGSVMSW
                     DYKYLVVQVADKLVTLDAKSDKVVYERFDHEYENGIMSISHDNKILATGGFDSKVNLY
                     NIATGNLISSIYTPRGKDFMLVNADGYYLAPKNTLEAVSFNYNNSTFGFEQFDVRYNR
                     PDIVLKGLGKADTGLINLFNQAWRKRLKKLNLNESEIGSEVHLPVVRMKDKFAVKPGT
                     SLREFTLTIECYDAKYPLQSLQVLVNNNSIFGSAGKLIPGNVNRTEIEVKIPLSPGNN
                     SLKIFCTNTKGAMSLSENMVVNSTYKLVEKMKTYFIGIAVSNYKDSTMNLRYAAKDVR
                     DLAVSFSSLFTNIEIDTLVDANATKENITALRKKLLGTTINDRVIISVNGHGLLSDSL
                     DFYFATHDVDFNKPQLRGLKYEDLEGLLDGIPARKKLLLIDACHSGALDKEEILARQE
                     DNQVILKDTSADKGVITGVASRGIIIEKSQSALDANS"
     assembly_gap    2856..2865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2866..3171
                     /locus_tag="PK977_03540"
     CDS             <2866..3171
                     /locus_tag="PK977_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17209.1"
                     /translation="FEVMQNLFADISSGNGAVIISAAGGMEYAFESDKWNNGVFTYCI
                     RKGIEEDLADKDGGDNNKVVTVSELKNYVSRKVSELTGGLQKPVSRKENIEFDWIVW"
     gene            3256..4494
                     /locus_tag="PK977_03545"
     CDS             3256..4494
                     /locus_tag="PK977_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17210.1"
                     /translation="MKYISFLSALIFCTFSLLAQRTLIAPAKQERIFPQWVGGDRDFW
                     GHGPRVTGTVRVEVTEGKGQIIAFLNLRLEETEGDGSAAEINETRLIYNAPAGKQIRS
                     IITPGPLVSEFNTKLAKGGLNRVGPASRIGPASHLIVNGDGESKDIGNNTNDDSYVSV
                     FFNGFVVELEPLPSGVREYNLAKSLLSSIVAAKLNGTKGRLNTYGPRVGDSWFKPNDS
                     WLKFPDEIRRDTMFFTDMKEVLISPRRYNYNDINLRNIRGDVNQQYLRLSVNWESDGP
                     EFRGECVNDVGCMFGSPSVQLDNFSIKINVRPFVKGSRLLYDPWDIQVDFGYNYSADC
                     GVLSALCTEIFKDPVQNAFFNARFMLASVLREAATLDQISVALTDGIMTFLRVPSRFP
                     GATQIVEVMDRGNDISIRCR"
     gene            complement(4551..5273)
                     /locus_tag="PK977_03550"
     CDS             complement(4551..5273)
                     /locus_tag="PK977_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRF17211.1"
                     /translation="MGRIFEVRKASMFARWDRMAKQFTRIGKEIAIAVKAGGPDPSTN
                     AALRRCFQNAKSVNMPKDRVEAAIKRAMGKEMENYDEILYEGYGPHGVAILVETATDN
                     HVRTVANVKSHFNKGGGTLGNSGSVSFQFKKMGVFKLKPEGLNIENLELELIDNGLEE
                     MGEGTGENGEDIIVLRCGFTDFGNMQKALEDKGLTPISAEVEWIPSTTVPLTDAQAED
                     VSKLIEKLEQDDDVQKVFHNMG"
     gene            complement(5373..>7008)
                     /locus_tag="PK977_03555"
     CDS             complement(5373..>7008)
                     /locus_tag="PK977_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF17212.1"
                     /translation="KPMDALMSEAAETGTHTLKKTLGAKGLIALGIGAIIGAGLFSIT
                     GMAAANHAGPAITISFVVAGLGCLFAGLCYAEFASMIPVAGSAYTYSYATMGEFMAWI
                     IGWDLVLEYAVGAATVGISWSRYLVKFLDGFGVALPTELTAGPWSGGIINLPAVFIIV
                     LMSLLLIRGTKESAFVNSIIVIVKIAVVLIFIILGWQYINNDNYTPYIPDNTGKFGDF
                     GFSGIIRAAAIVFFAYIGFDAVSTAAQEAKNPKKDMPIGILGSLLICTILYILFAHVM
                     TGVTSYTTFKGQDGIAPVAVAIEHMGSPDAAGVYHADYPWLNRAIVVAILFGYASVIL
                     VMLMGQSRVFFSMSKDGLIPKIFSAVNPKTQTPAKNNFLFMLFVSAFAAFVPADVVGE
                     MTSIGTLFAFILVCIGVWVMRKKMPDVPRAFKTPMVPLVPLLGVATCLFMMIFLPMDT
                     WIRLLVWMLIGLDIYLVYGAKNSHLGNGTSSRKGMRMARYTGIVLAVLLAIVGFLHQN
                     SVGFDTDRTLFYISVAFAVFHAGLYASKLGRAEDKS"
     assembly_gap    7009..7038
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7078..8517)
                     /locus_tag="PK977_03560"
     CDS             complement(7078..8517)
                     /locus_tag="PK977_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF17213.1"
                     /translation="MSDQHTSFKPTLSLFDGTMIVAGSMIGSGIFIVSADIARNTGSA
                     GWLIAVWLITGFMTLTAALSYGELSGMFPKAGGQYVYLKEAYNPLMGFLYGWSFFAVI
                     QTATIAAVGVAFSKFLAYLVPELGNSYFLFDAGITKIYSGQLVAIFIICLLTYINSKG
                     VKEGKFIQTLFTSAKLLALFGLILFGFILVKESFWAENWKTGFSAMQDFGQKDATGQL
                     QPTSWTAISGSVLMGAIAAAMVGSIFSSDAWTNVTFIAGEMKNPKRNIGLSLFLGTLI
                     VTIIYVSVNLMYLNVLPLDELAFAKDDRVAVVAANKIFPSFGTILIALLIMVSTFGCN
                     NGLILAGARVYYTMAKDGLFFKNVGELNKNAVPQLALWIQCIAASIWSLSGKYGQLLD
                     MISFVVVLFYMLTIAGIFILRKKRPDAERPYKAFGYPFLPVLYILMGAAFCTLLIIYK
                     PQFTWPGLIIVLLGVPLYYLAISANKKQS"
     gene            8670..10061
                     /locus_tag="PK977_03565"
     CDS             8670..10061
                     /locus_tag="PK977_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRF17214.1"
                     /translation="MNRRRSGQLALLLFTIVSILHVIYWYNIAAVPETALSVSRWVFI
                     AALCVYAWYKKSLTAWIMVSMVLGVLIGYEYPAFSQDLKFLRQIFLNLVKAIVAPLLF
                     STLVGIAGHSNLKQVGRLGWKSLLYFEIVTTIALIIGLVAINISQAGTGIIQPEVLEK
                     LPETKPLSWDEHIVDIFPSNFIKAIYDGKVLQIVVFSVLFAIGLAMLKEEKKRPMLRA
                     CESLSEAMFKFTNIIMYFAPIGIGAAMAFTVGHLGIDILKNLFQLVLTLYAALIVFLF
                     GVLLPVALMIKLPIKKFVQAIREPVSIAFATTSSDSALPKAMENMEKFGVPRKIVSFV
                     IPTGYTFNLDGTTLYLSLASVFVAQAANMHMSIGTQITMCLVLMLTSKGVAAVPRASL
                     VILIATADQFQLPLWPIAAIFGIDELMDMARTSVNVIGNTLASCVIARWEGEFDDKKA
                     LAFSEREADAIRD"
     gene            10085..10840
                     /locus_tag="PK977_03570"
     CDS             10085..10840
                     /locus_tag="PK977_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRF17215.1"
                     /translation="MDLILLDAFHWTNLLQPQWYVENGGLWLLLFVVFAETGLFVGFF
                     LPGDSLLFVSGIFAHEITTKTGETGPGLGYQFLKYFGFDYPYTYWLDLFVLVGLISLC
                     GIAGNAVGYWTGRKVGPAMYHWRDRMLFKKRYLHQAHDFYEKHGGLAVIGARFLPFIR
                     TFAPIVAGIVEMDRRKFHFYNIVGCLAWVITMVLGGFFLQKWILQQFGFDLKEHLEII
                     VLGIVLVTTAPVIIKMISGRKKPLPPPPGNSNV"
     gene            10847..12091
                     /locus_tag="PK977_03575"
     CDS             10847..12091
                     /locus_tag="PK977_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF17216.1"
                     /translation="MNQQKNYGILSIPVIVAALGYFVDIYDLLLFSITRIESLRSFNL
                     TPEQITSEGEGIIMWQMAGLLIGGIIWGIMGDKKGRLSVLFGSIVLYSLANIANGFVE
                     NTLQFRIVRFIAGLGLAGELGAGITLVSELIPKNKRGIATSFVAGIGLTGAVVAFIMK
                     QNFDWRTCYFIGGGMGLGLLLLRISVFESGMFHEVKKMDVQRGNLLMLFNNRDRFKRY
                     FLGILIGLPTWFVIGVLVSFSKEFGKHFGIHGEIDPGRAIMYAYAAISVGDILIGFVS
                     QWFRSRKKALYLFYCITAVFMVLFFTVQWNGSPSMMYWICAGLGFGTGFWAIFVTMGA
                     EQFGTNLRATAATTIPNMVRGMLTIFILPPFQGLRMVTDYVTAGWIAAIIIMVITVIA
                     ALFTQETFNKDMNFLELEEMTP"
     gene            12099..13085
                     /locus_tag="PK977_03580"
     CDS             12099..13085
                     /locus_tag="PK977_03580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRF17217.1"
                     /translation="MPKFLIIRFSSIGDIVLTTPVIRCLKQQVPDAEVHFLTKSSFLP
                     VVQHNPYIDKIHLLAHSWELMIEELKSENYDYIIDLHHNTKTLRVKSALKKKSFSFYK
                     LNIEKYIYTSLKLNILPKVHIVDRYLKTVESFGVKNDGAGLDYFISKEEETKREDIPA
                     SHSAGFVACVIGAALGTKQWPVHKWKEFCLQLNHPVILLGGPEDVTAGNEIAAVDDIK
                     VYNACGKFKLNESADLVRKAKLVVTNDTGLMHIAAAYKKPIISLWGNTVPSFGMTPYY
                     GVSMVPHTMVQVNKLWCRPCSKIGYKKCPLGHFKCMEKIEVEQVIQIVKQSL"
     assembly_gap    13404..13419
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13420..14323
                     /locus_tag="PK977_03585"
     CDS             <13420..14323
                     /locus_tag="PK977_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17218.1"
                     /translation="FSLQAAGQKGKSIIDSFWVYGDIKVTIDKPSDFSAFRKAIVVFY
                     ALPNGNTTEQTMGKKMQPGDDWHFDIQHIKAQTSFIRQQLKNKNFIVVYLENSYKSWP
                     AWKQKHSGYRDTIPRLIDSVSSVFSKRKQVYLNGHSGGGSFIFGYLAGVKIIPKYVKR
                     ISFLDSNYGYDSSYYTKIKNWLLRNSDAALNVFAYNDSVALYNGKSVVSATGGTWYRS
                     HLMLKDMDDHFSFHHSTTDSMKIARSKGNKIQFFLKYNLNKGIYHTQQVELNGFIHSV
                     LCGTKYDSQRYRYYSIRAYSEFIE"
     gene            complement(14326..>14910)
                     /locus_tag="PK977_03590"
     CDS             complement(14326..>14910)
                     /locus_tag="PK977_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRF17219.1"
                     /translation="DAHLQYKTSNDQGLWLILGNLNFLKIEKNRFLSDGLLHLRYNRK
                     VNEWLRWEFFGQFQNNEVTQIDSRLLFGTGPRFKLAKAKTFKLYAASLFMYEREKEAT
                     DPVIRHSDFRNSSYLSFTWLPKDYLELISTSYFQPLLKKFSDYRIMNQFVFRVKATPH
                     FSLTLKWNYLHDRFPAGTAPKTIYNFSTGFTYDL"
     assembly_gap    14911..14920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14921..15109)
                     /locus_tag="PK977_03595"
     CDS             complement(<14921..15109)
                     /locus_tag="PK977_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17220.1"
                     /translation="MKLKTLSVYCLLLYIFILDTEKSAAQIVNVESARMQSDTVGWMG
                     SFGTSFTMNKNKVKIFGAS"
     gene            complement(15134..>15572)
                     /locus_tag="PK977_03600"
     CDS             complement(15134..>15572)
                     /locus_tag="PK977_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF17221.1"
                     /translation="ENFLSEFPGCLLIVSHDRYFMDRLVDHLFVFEGDGAIRDFPGNY
                     SQYRLQQTKDEPSIKKPEEKQTEQPAKEKNRRQLTFKEKREFELLEKEISDLSKEKEV
                     ITEKLNSGSTPFEQLQQLSVRIGEVTRLLDEKELRWLELSEIE"
     assembly_gap    15573..15647
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15648..17004)
                     /locus_tag="PK977_03605"
     CDS             complement(<15648..17004)
                     /locus_tag="PK977_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRF17222.1"
                     /translation="MHYVSVEGLTKSYGIQPLFSAISFHIEEGDKIALVARNGTGKST
                     LLRILAGKETADEGKVWINKDVDVVLFEQDPSFVEELSVLDNIFFHNHPVINAIKEYE
                     AVSETNDAEKLSSTIIKMDELGAWDFDTKVKQVLGKLNIHHLSQPVNTLSGGQRKRVA
                     LAKTLIDIGFDHKHVLLIMDEPTNHLDVEMVEWLEHYLDKENVTLLLVTHDRYFLDAV
                     CNEIWELDGSNMYVYKGDYENYLDKKAARIESDAASIDKARNLYRKELEWMRKQPKAR
                     TTKSKSRQDNFYEVEKKAKQQVADEQMQLDMKMNRLGGKVIELKKVYKSYGDKIILKG
                     FDYTFKKGERIGVVGKNGVGKSTFINLLQGIEKADSGKINVGDTVIFGNYSQQGLVIK
                     EDMRVIEFVKSMAESFPLAGGGSLSAAQFLQLFLFDPDKQYTYISKLSGGEKRRLHLL
                     SI"
     gene            complement(17020..>17528)
                     /locus_tag="PK977_03610"
     CDS             complement(17020..>17528)
                     /locus_tag="PK977_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17223.1"
                     /translation="EIKEAYEVLTDPAKKEYYLQQRWYNQSMGYQKTQGIVTPVSLLK
                     QSLELEKYVSRLDVFRMDKEKLKDYLMEMLSDNVIDKIKAFNEHSTTKSIIAILLKTI
                     QPLSSEMSDPVIRQLFKLADQNESEILLIKNSVANTQKRDRLEKYKPLVILFFTLLIC
                     GLIWLMSR"
BASE COUNT         5057 a         3369 c         3689 g         5272 t
ORIGIN      
        1 gaatattaaa agtttgggac ctggctacag gtagaaccat ttcatcattt actgctcacc
       61 agttggagat aaattctgtt caatacagcc ctaacggacg gtatatagcc acttcttctg
      121 aagacagcac tatcaaagta tggaatgcct atgatttttc attattatat acgatcaaaa
      181 ggccaaagcc tgagtctgat gtaatttttt cgccagacag taaatttttg gtagcaggag
      241 gaagagattc gcttatcaag tttatcagta ttcactccgg caaagtattg aaaacactta
      301 aaaaacacag aggaacggtt acatccttat gttttatgcc ttctgacaac gactatcttt
      361 tttctggcgg tgctgatagt gttgtttata aatggaattt gaccaccaat gactgggatc
      421 attggtatgg tgcaaaaggc agggttatgg atgtgtctgt gtccaatgat ggcaagtacc
      481 tcagttttgt ttcttctgat acgcttatca gggtttggaa actggagaac aataaatttt
      541 atttcaatat aagacccaat tattcaacta ctgatccggt tgatattgct tttccatctt
      601 tttcacccga cagcaggtac atggctctgg cgctaaaaaa tgacacatta gatataattg
      661 aattgtcaac attaaaggag caggtgtatg gctttaaaat ggaaaagttc cagggattgt
      721 atgatatggt ctttagtcct gatggtgctt cattggccag ccgcatgagc ctgggagggc
      781 cattaagggt attcaatttc tccggctggg attttcattc taatgctact gtaaattcaa
      841 aggacctgaa gaattattct aacctgccat tatctgtaca atttacaaaa gatgataatg
      901 aactggtgat tgtacatgat gctgtttcta aaattgacct tcgaaacgga gctaccactc
      961 atttatataa tgagtcatta ttttttttga acaacaacat tctgttgaat gatggaaaga
     1021 caggtttgta taatgacctg gttcggcccg gcttgaaatt ttatgacata ccttcaaaac
     1081 aagttaagct ttcgctaagc cttcctgaca aatctgaaga attgcgaagg tttgaattgt
     1141 cagctgataa tagccttatt tttattggag gaatcaacaa tagtattgct ggtttttctc
     1201 tgccgggagg agggcaatta ttttctgcta aatataacct gggtgaccgt gagagtttga
     1261 ttttcttgcg atatgacagt atacgaaacc gtttatatgc cattggaaaa aaccaggaag
     1321 tactgatcat tcatccgcaa acaggggact caatagcgtc tattaaagca gcttttcccc
     1381 agacaataga ggtaacaccg aagtatcttt ttgtaacctg tgattccagt ctggtgtata
     1441 aatatgatgc aaataattta cgcctgttaa agaaaatacc tgttcataag agtaaaagta
     1501 attgctatgg ctcagtaatg tcatgggatt ataaatacct cgttgtacaa gtggcagata
     1561 agttagttac actggatgca aagtctgata aagttgtata tgagagattt gatcatgaat
     1621 atgagaacgg aattatgagc atcagtcatg acaacaaaat actggcaaca ggggggtttg
     1681 acagtaaagt gaatttgtat aatatcgcaa caggaaatct gataagcagc atatatacac
     1741 cacgggggaa agattttatg ctggtcaatg ctgacggata ttacctggct ccaaagaata
     1801 cactggaggc agtttctttt aactataaca atagcacatt tggttttgaa caatttgatg
     1861 ttcgttataa ccgcccggat attgtattaa aaggtctggg caaagctgac acagggctga
     1921 taaatttatt caaccaggca tggagaaagc gattgaagaa attaaacctg aatgagtcag
     1981 aaataggtag tgaagtacat ttaccggtag taagaatgaa agataagttc gctgtaaaac
     2041 caggtacatc actcagggaa tttacactga ctatagaatg ttatgatgcg aaatacccgt
     2101 tgcaatcgct gcaggtactg gtgaataata attcaatatt tggctcagcc ggtaaattga
     2161 ttccgggtaa tgtaaacagg acagaaattg aagtgaaaat acccttatcc ccggggaata
     2221 acagcctcaa gattttctgt acgaatacaa aaggggctat gtctctttca gagaatatgg
     2281 ttgtaaatag cacttataag cttgtagaaa agatgaaaac atatttcata ggcattgctg
     2341 tttccaatta taaagacagc actatgaatc tacggtatgc tgccaaggat gttagggatc
     2401 ttgccgtatc gttctcttcg ttgtttacaa atattgaaat tgataccctg gttgacgcca
     2461 atgcaacaaa ggaaaatatt actgcattga ggaaaaaatt attaggaaca acgattaatg
     2521 accgggtgat tatttcagtg aatgggcatg gattactaag cgacagcctt gatttttact
     2581 ttgccaccca tgatgtcgat tttaataaac cacaactacg tggtttgaag tatgaagacc
     2641 tcgagggctt gctggacgga attccggcga ggaaaaagtt gctgctgata gatgcctgtc
     2701 attccggggc attggataaa gaggaaatat tagcccggca ggaagataac caggttatat
     2761 taaaagatac ttcggcagat aaaggagtga taacgggtgt ggcatcccgt ggtattatta
     2821 ttgagaagtc acaatcagct ttagatgcca atagcnnnnn nnnnntttga agtgatgcag
     2881 aatctttttg cagatatttc ttcaggcaat ggcgctgtga tcatttctgc tgccggcggt
     2941 atggaatatg cgtttgaatc ggataaatgg aataatggag tatttactta ttgtataaga
     3001 aagggtattg aagaagatct cgccgataaa gatggcggag ataataacaa ggtcgttacg
     3061 gtatctgaac ttaaaaatta tgtgagccgg aaagtaagtg aacttaccgg tggtttacaa
     3121 aagcccgttt caagaaaaga aaatattgag tttgactgga ttgtatggtg aagggaatga
     3181 gtttttacgt ggaaaactcc cgttgaaatt aactggaata ctggttaact ttaatagaac
     3241 cttaaaacca gtgttatgaa atacatctcc tttttatctg cgttaatttt ttgcacattt
     3301 tctttattgg ctcaacgtac tttaattgcc cccgccaagc aggagagaat atttccgcaa
     3361 tgggtcggag gtgacaggga tttttgggga catgggccaa gagtgaccgg tacagtgagg
     3421 gttgaggtaa cagaaggaaa gggacaaata attgcatttt taaatttgag actggaagaa
     3481 acagaaggtg atggaagtgc tgcagaaata aatgaaacaa ggcttattta taatgcacca
     3541 gccggaaagc aaattcgttc aataataacg ccgggtccgc tggtaagtga gtttaataca
     3601 aagcttgcaa aaggaggact gaacagggta ggccctgctt cccggatcgg accggcaagc
     3661 catttaattg taaatggtga tggcgaaagt aaagacatcg gcaataatac aaatgatgat
     3721 agttatgtca gcgttttttt taacggtttt gttgttgaat tggaaccgct gccttcagga
     3781 gtgagagagt ataatcttgc caaatcatta ctctcatcta ttgtagctgc caagctaaac
     3841 ggtacaaaag gccggttgaa tacctatggt ccgagagtgg gagactcctg gtttaagccc
     3901 aatgattcct ggttaaaatt tccagatgag ataagaagag ataccatgtt ttttactgat
     3961 atgaaagaag tcttgatatc tccaagaaga tataattaca atgatattaa tcttaggaat
     4021 ataaggggag atgtaaatca gcagtatctc cgcttaagtg taaattggga aagtgacggc
     4081 ccggagttca ggggagaatg tgtgaatgat gtaggttgta tgtttggttc accttctgta
     4141 caacttgata acttcagtat caagattaac gtcagaccgt ttgtaaaggg gagtagattg
     4201 ttgtatgatc catgggatat tcaggttgat tttgggtata attatagtgc agattgtggt
     4261 gtactctctg ctctatgtac tgaaattttt aaagatcctg tacaaaatgc attttttaat
     4321 gcccggttca tgctggcatc tgtattacgg gaggcagcta cgctagacca gatatcagtt
     4381 gcacttacag atggaataat gactttttta agagtgccta gcagattccc cggagctact
     4441 caaattgtag aagtgatgga cagaggtaat gatatttcga ttaggtgccg gtaattaaaa
     4501 tgacttaaat aaaaaaccgg attcattatc cggtttttta ttttaatagt ttatcccata
     4561 ttatgaaaca ccttttgtac atcatcatcc tgttccagtt tttcaatcag tttgctaaca
     4621 tcttctgctt gtgcatcagt taagggtacc gtagttgagg gtatccattc cacttctgca
     4681 ctgataggag tgaggccttt atcttccagg gctttttgca tattaccaaa atccgtaaag
     4741 ccacaacgaa gtactattat atcctcacca ttttcaccgg taccttctcc catctcttcc
     4801 aggccattat cgatcagttc cagctcgagg ttctcaatgt tcaatccttc gggtttcaat
     4861 ttaaaaactc ccattttctt gaactgaaag gaaacgcttc cactattgcc aagcgtcccc
     4921 ccgcctttat taaaatgtga ttttacattg gctactgttc ttacatggtt atccgtagct
     4981 gtttcaacaa gtattgctac gccatgcggt ccatatcctt catagagaat ctcatcatag
     5041 ttctccattt cttttcccat agcccttttg atagctgctt ctactctgtc tttgggcata
     5101 ttcacactct tggcattctg gaaacaacga cgcagggcag cgttggtgga agggtcgggg
     5161 ccaccggctt tgaccgcaat ggcgatctct ttaccgatac gggtaaattg tttggccatc
     5221 cggtcccacc gtgcaaacat cgaggcttta cgtacttcaa aaattcttcc catggttcaa
     5281 ttcacttttg aggctgcaaa tctacgggaa tgggggtaaa agaaaaaccg ccccgcaagc
     5341 cgggacggtt taaccagtta ttggcaaaca aattaactct tgtcctctgc tcttccaagt
     5401 ttgctggcat aaagccctgc gtgaaaaaca gcaaaagcaa cagagatata gaataatgtt
     5461 ctgtcagtat caaaacctac agaattttga tgtaaaaacc cgactatcgc taagagaaca
     5521 gccagaacaa tacctgtgta gcgggccatt ctcattccct tcctgctgct ggttccgttt
     5581 ccaaggtgac tgtttttagc accatatacc aggtaaatat caaggccaat cagcatccat
     5641 accagtaaac ggatccaggt atccatcggc agaaatatca tcataaataa acaggttgcc
     5701 acacctaata aggggacaag aggtaccatt ggagttttaa aagctcttgg tacatctggc
     5761 attttcttac gcatcaccca cacaccaata caaaccagga taaaggcaaa caatgttccg
     5821 atacttgtca tctcacccac gacatctgca ggtacaaatg ctgcaaaagc acttacaaat
     5881 agcatgaaca ggaaattgtt tttagccggt gtttgagttt ttggatttac tgctgagaaa
     5941 attttgggga tcaatccatc tttactcata ctgaagaata cacggctttg tcccatcagc
     6001 atcactaata ttactgatgc ataaccaaac aagatggcca caacaatggc acggtttaac
     6061 catggatagt cggcatgata gacacctgca gcatctggag atcccatatg ctctatcgca
     6121 acggcaacag gagcaattcc atcttgcccc ttaaatgttg tatagctggt aacccccgtc
     6181 atcacatggg caaaaagaat atacaatatg gtacaaataa gcagcgaccc taaaatacct
     6241 ataggcatat ccttttttgg attcttggct tcctgtgctg ctgtactaac tgcatcaaac
     6301 ccgatatagg caaaaaatac gatggcagct gcccgtatga tgccgctgaa accaaaatcg
     6361 ccaaacttgc ctgtattgtc agggatataa ggagtgtaat tgtcgttatt tatgtactgc
     6421 catccaagga taatgaaaat gagtaccacc gcaattttta caattacaat aatactattc
     6481 acaaatgcac tctcttttgt tcccctaatc agcagtaaac tcattaatac gataatgaat
     6541 acagcaggaa gattaatgat gcccccgctc cagggacctg ctgttaactc tgttggtaat
     6601 gcgaccccaa acccatcaag aaatttgaca aggtatcggc tccagctaat tcctaccgtg
     6661 gctgcgccta ccgcatattc aagcacaagg tcccagccaa tgatccaggc catgaattct
     6721 cccatggtag cataagagta ggtgtaggca ctaccagcaa ccggaatcat cgaagcaaac
     6781 tcagcataac acaaaccagc aaataaacaa cccaggcctg ccactacaaa tgagattgtg
     6841 attgctggcc cggcatgatt ggctgcagcc atacctgtaa tggaaaataa accagctcca
     6901 ataatggctc cgattccaag ggctattaac ccctttgctc ccaatgtttt tttaagcgta
     6961 tgtgtaccgg tttctgcagc ttcgctcatc aaagcatcca tcggttttnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnntt ttattaagtg actaagataa gtattgaaac caaaattcta
     7081 ggattgtttt ttattggcgg aaatggccag gtaatacaaa ggaactccta agagtacaat
     7141 gatgagacca ggccaggtaa actgtggttt gtaaatgatc aggagagtgc aaaatgccgc
     7201 acccattaat atgtataaaa caggcaaaaa ggggtagcca aaggctttat aaggtctttc
     7261 ggcatccggc ctttttttgc gaaggataaa aataccggcg atggtaagca tataaaacaa
     7321 tactaccaca aaagagatca tatccagcaa ttgcccgtat ttaccactca ggctccagat
     7381 gcttgctgca atgcattgta tccataatgc cagttgggga acagcatttt tatttaactc
     7441 gcctacgttt ttgaagaaca acccgtcttt tgccatggta taatataccc gggcgccggc
     7501 cagtatcagc ccgttgttac agccaaatgt agaaaccatg atcaataacg ctatcaggat
     7561 ggtgccaaaa gaaggaaata tcttattagc tgccaccact gccacccggt catctttggc
     7621 aaatgccagt tcatccagcg gcagcacatt caggtacata aggtttacgg atacataaat
     7681 aatggtcaca atcaaggtgc ccagaaaaag gcttaagcct atattccttt tcgggttctt
     7741 catttcaccg gcaataaatg ttacattggt ccaggcatcg ctgctgaaga tagaaccaac
     7801 catggcagct gcaatagcac ccatcagtac actgccagaa atagcagtcc aggaagttgg
     7861 ctgtagctgg ccggtggcgt ctttttgccc aaagtcctgc attgcagaga aacctgtttt
     7921 ccagttttca gcccaaaaac tttcttttac cagtataaat ccaaagagaa ttagaccaaa
     7981 caatgctaaa agcttggcag atgtaaataa tgtttgtata aacttacctt ctttgactcc
     8041 ttttgaatta atataagtta gcaggcaaat gatgaatata gccactaatt gtcctgaata
     8101 aattttagtg ataccagcat caaaaaggaa ataactgttg cccaactccg gaaccaggta
     8161 agccaaaaat tttgagaagg caacacccac tgctgcaatg gtggcagttt gaatcacagc
     8221 aaaaaaactc cagccatata aaaatcccat cagcggatta taggcttctt tcaaataaac
     8281 atattgtcct cctgcttttg gaaacatacc gctgagttcc ccatagctga gtgctgcggt
     8341 aagcgtcata aacccagtga tcagccatac agcaataagc catccggcac tgccggtatt
     8401 gcgggctata tctgcactaa cgatgaaaat accagatcca atcatagatc cggcaacgat
     8461 catagtacca tcaaataagc tgagagtggg tttaaaagag gtgtgctgat cggacatttt
     8521 atttgttggt ttgggttgat tctataatat gaagagggaa aaatacagat ttccgctaaa
     8581 agcggggcaa aagaattacc ggtggttatt tcatgggatt ttcccaattg accgtaaacc
     8641 tttagttttg aaacttcaca gtttaaacta tgaacagacg ccgttccggg cagcttgcct
     8701 tactgttatt cactatcgta tctatactgc atgtaatcta ttggtacaat attgctgccg
     8761 ttccggaaac agcactttcg gtcagcaggt gggtatttat tgcggcattg tgtgtgtatg
     8821 cctggtataa aaaatcactt accgcatgga tcatggtatc tatggtacta ggagttctta
     8881 ttggctatga ataccccgct ttttcacagg atttaaaatt tttgcgacag attttcctga
     8941 acctggtgaa ggcaattgtg gcaccgttat tattctctac cctggttggt attgccgggc
     9001 attctaactt aaaacaggtt gggaggctgg gctggaaatc attgctctat tttgaaattg
     9061 taacaactat tgctctgatt attggcctcg ttgccattaa tatttcacag gctggaacag
     9121 gcattataca gcccgaagtg ctggagaagc tgcccgaaac caaaccatta tcatgggatg
     9181 aacatatcgt tgatattttt ccttccaatt ttataaaagc aatttatgat ggaaaggttt
     9241 tacaaattgt tgttttttct gttctgttcg caataggcct tgccatgttg aaagaggaaa
     9301 agaaaagacc aatgctcagg gcctgtgaga gtttgtccga agccatgttt aagtttacta
     9361 acatcatcat gtactttgcg cccattggta tcggtgctgc catggcgttc accgttgggc
     9421 atcttggaat agacattttg aaaaaccttt ttcaactggt attaacatta tatgccgcat
     9481 tgattgtttt cctctttggt gtattactgc cggtggcttt aatgataaaa ctgccaataa
     9541 aaaaatttgt acaggctatc agggaaccag tttctattgc ttttgccact accagttctg
     9601 attctgcgct tcccaaggcg atggagaata tggaaaaatt cggggtgcca agaaaaattg
     9661 tatcctttgt cattccgacc ggttatacat tcaatctaga tggcaccaca ttataccttt
     9721 cccttgcttc ggtttttgta gcgcaagcgg ctaatatgca tatgagtata ggcacacaaa
     9781 ttacaatgtg cttagtactg atgttaacca gcaaaggtgt ggcagctgtt cccagggctt
     9841 cgttagtgat attaattgca acggctgacc agtttcagct tccattatgg ccgattgctg
     9901 ccatttttgg aattgacgaa ttaatggata tggcccgcac ttctgtgaat gtgattggta
     9961 atacactggc tagttgcgtt attgccaggt gggaaggtga gtttgatgat aaaaaggcgt
    10021 tagcttttag tgaaagagaa gctgatgcca tcagggatta aataaaaaaa gtaaaagagg
    10081 aaaaatggat ttgatattgt tagatgcttt tcattggaca aatttattac agccacagtg
    10141 gtatgtggaa aacggtggtt tatggctgct gctttttgtt gtttttgcag aaacaggttt
    10201 atttgttggc ttcttccttc cgggagattc tttattgttt gtttcaggaa tttttgctca
    10261 tgaaataaca accaagaccg gtgaaacagg tccgggttta ggataccagt ttttaaaata
    10321 ctttggtttt gattatcctt acacatactg gcttgattta tttgtgctgg tgggcctgat
    10381 ctccctctgc ggtattgccg gcaatgccgt tggttattgg acgggccgta aggtaggtcc
    10441 ggccatgtat cattggcgtg acaggatgct tttcaagaaa agataccttc accaggccca
    10501 tgatttctat gaaaagcatg gcggtcttgc tgtgatcggg gcccgatttt taccttttat
    10561 cagaaccttt gctccgatag tcgcaggtat agtggaaatg gacaggagga aatttcattt
    10621 ttataatatc gttggatgcc ttgcctgggt tattacgatg gtgctgggcg gattcttcct
    10681 gcaaaaatgg atattgcagc agtttggatt tgacctgaag gagcatttgg aaataattgt
    10741 attgggaatt gtattagtaa caactgcccc ggtaatcatc aagatgatct ctggaagaaa
    10801 gaaaccactg ccacctccac ccggaaattc aaacgtttaa aagaggatga accagcaaaa
    10861 aaactatggc atactttcta tcccggttat tgtagcagcg cttggctatt ttgttgatat
    10921 ctatgatttg ttgcttttta gcattacaag gattgagagc cttcgttcat tcaatttaac
    10981 cccggaacaa atcacttcag aaggagaagg tatcatcatg tggcaaatgg cgggtttgct
    11041 tattggcggc attatctggg gaataatggg agacaaaaag ggacgattaa gtgttctttt
    11101 tggttcaatt gttctttatt cactggcaaa tattgccaat ggctttgtag aaaacacttt
    11161 acagttccgc atagtaagat ttattgccgg gctcggactt gcaggtgaac tgggtgccgg
    11221 tattaccctg gtatccgaac taattcccaa aaacaaaaga ggaattgcca cttcgtttgt
    11281 agccggcatt ggactgactg gagccgtggt ggcttttatt atgaaacaaa attttgactg
    11341 gcggacctgt tattttatcg gtggtggtat ggggcttgga ttattgttac tgcgtatttc
    11401 agtttttgaa tccggaatgt ttcatgaggt taaaaaaatg gatgtacagc ggggtaattt
    11461 gttgatgctg tttaataacc gggatcggtt taagcgatat ttcctgggta tactaattgg
    11521 cctgcctacc tggtttgtca tcggtgtgct ggtttctttt tcaaaagagt ttggaaaaca
    11581 ttttggtatt cacggggaga ttgatcccgg cagggccatt atgtatgcgt atgctgctat
    11641 ttcggtggga gatatcctga taggctttgt cagccagtgg ttccgcagcc gtaaaaaagc
    11701 actatatctt ttttattgca ttactgcagt gtttatggtt ttgtttttta cggtacaatg
    11761 gaacggaagc ccgtcaatga tgtactggat ctgtgcagga cttggctttg gtacaggttt
    11821 ttgggcaatt ttcgttacca tgggtgctga gcaatttggc accaatcttc gggctacagc
    11881 ggctactacc attcccaata tggtcagagg tatgcttacc atctttattc ttccgccttt
    11941 ccagggcctg cgtatggtga ctgattatgt tactgccgga tggatagctg ccattatcat
    12001 tatggtgata acggtaattg cagcactatt tacccaggaa actttcaata aggatatgaa
    12061 cttccttgag ctggaagaaa tgacaccata aatatcgagt gcctaaattt cttatcatac
    12121 ggttttcttc tatcggtgat attgtactca ccactccggt cattcgatgc ctgaaacaac
    12181 aggtgcccga tgcagaggtt cattttttga caaaatcttc ctttctcccg gttgttcagc
    12241 acaatccata tattgacaaa atacatttgc tggcacatag ctgggagtta atgatcgaag
    12301 aactgaaatc agagaactac gattacatta ttgatttgca tcataacacc aaaacactgc
    12361 gggttaaatc ggccttgaag aaaaaatcat tttcctttta taagctgaat attgaaaagt
    12421 atatctacac cagtctgaag ctgaatattt taccgaaagt gcacattgta gaccgttacc
    12481 tgaaaaccgt agaatcgttt ggagtgaaaa acgatggggc cgggcttgat tattttattt
    12541 caaaagagga agaaacgaaa agagaagata ttccggcctc acattctgcc ggttttgtgg
    12601 cttgtgtaat tggtgccgcc ctgggtacca aacaatggcc ggtgcataag tggaaagagt
    12661 tttgcctgca attaaatcat cctgtgattt tattgggagg ccctgaagat gtaaccgcag
    12721 gaaatgaaat tgctgcagtg gatgatataa aggtttacaa tgcctgtggt aaattcaaac
    12781 tgaatgaaag tgccgacctg gtccggaaag caaaactggt ggttaccaat gataccgggc
    12841 tgatgcatat agctgctgca tataaaaagc ccattatctc cctatggggc aatactgttc
    12901 cttcattcgg catgacacct tattacggtg tgagtatggt gcctcacacc atggttcagg
    12961 ttaataaact atggtgccgt ccctgcagca agatagggta taagaaatgc cccctcggcc
    13021 acttcaaatg catggaaaaa atagaagtgg agcaggtgat tcaaatcgtg aagcagagtt
    13081 tataatctgc cggtttttag aagaagaaca atgttaccaa accaaacatg atcacaaaac
    13141 ctgcgatcac cagcctggcc tgtacatctt cattctgtaa catgagtcga aattttttca
    13201 taacctgatt ttagttttca aaatagattt gagtaatcag gtttttccgg agtggataaa
    13261 caggtattcc aaaaggatag gagcggagta aattgctgcc gtaaaaatag aatgcaaaaa
    13321 agttatttgc aactaaacac taataggaaa tctacgatgc agggtaaaaa aatttggctt
    13381 atctgggtat ctttaataat gcgnnnnnnn nnnnnnnnnt ttttcattac aggctgccgg
    13441 tcaaaaaggt aaaagcatta ttgattcttt ttgggtttac ggagatataa aagtaacgat
    13501 tgataagcca tctgattttt ctgctttccg taaagcgata gtagttttct atgcgcttcc
    13561 caacggaaat acgacggagc aaaccatggg taagaaaatg caaccagggg atgactggca
    13621 tttcgatatt cagcatatta aggcgcaaac aagttttata aggcaacagc taaaaaacaa
    13681 aaactttatt gtggtttacc tggagaatag ttataaaagc tggcctgcct ggaagcaaaa
    13741 gcacagcggt tacagggata caattccccg tttgatcgat tcagtttcgt ctgttttttc
    13801 caaaaggaaa caagtttacc tgaatggaca tagtggtggc ggaagtttca tttttggtta
    13861 tttggcagga gtaaagatta ttccaaagta tgttaagcga attagttttc ttgatagtaa
    13921 ttatggatac gatagtagtt attataccaa aatcaaaaac tggttattgc gcaacagcga
    13981 tgcggcactg aatgtttttg cgtataatga cagtgtggct ctgtacaatg gcaaatcggt
    14041 ggtatctgct accggcggaa cctggtacag gtcacacctt atgcttaagg atatggatga
    14101 ccatttttct tttcatcaca gcactacaga cagcatgaaa attgcacgca gcaagggtaa
    14161 taagattcag ttcttcttaa agtacaactt aaacaagggt atatatcata ctcagcaagt
    14221 agaattaaac gggtttattc attcggtttt atgcggaaca aagtatgact ctcagcgtta
    14281 tcgttattat tcaatccgtg cttatagcga gttcattgag taagactata aatcgtaggt
    14341 aaaaccagtt gaaaaattat agatcgtttt gggtgcagtt ccagctggga aacggtcgtg
    14401 taaataattc cattttaaag taagtgaaaa atgcggagtg gcttttactc taaacacaaa
    14461 ttggttcatg attctatagt cactgaattt tttaagaagt ggctgaaaat aggaagtgct
    14521 gatcagttca agatagtctt taggcagcca ggtaaatgaa agatagctgc tattcctgaa
    14581 atcactatgc ctgatgacgg gatctgtagc ttctttctcc ctctcgtaca taaacaaact
    14641 ggctgcatat agcttgaatg ttttagcctt tgccagttta aaacggggtc cggtaccaaa
    14701 gagaagacgg gagtctatct gtgtgacttc attgttttgg aactgaccaa agaactccca
    14761 gcgcagccat tcattcactt tcctgttata gcggagatgg agtaacccgt ctgagagaaa
    14821 ccgattcttt tcaattttca ggaagttaag attgcccagt atcagccata agccctggtc
    14881 gttactggtt ttatactgaa gatgggcatc nnnnnnnnnn actggcaccg aatattttta
    14941 ctttgttttt gttcatagta aaagatgtgc cgaagctgcc catccatccc actgtatcag
    15001 attgcatccg ggcactttct acatttacga tttgtgcagc gcttttttct gtatcgagaa
    15061 taaatatgta caacagaaga caataaacgg ataaggtttt taatttcatg cttgggggag
    15121 tataatagat tagttattcg atttcactta gttcgagcca acgaagttct ttttcgtcaa
    15181 gcaggcgggt aacttccccg atcctgacag aaagttgttg tagttgttca aaaggtgtgc
    15241 tgccgctatt cagcttttca gtgataacct ctttttcttt tgacaagtcg ctgatctctt
    15301 tttcaagtaa ttcaaattct cttttttcct tgaatgttaa ctggcgtctg tttttttctt
    15361 tagcaggttg ttcggtttgt ttttcttccg gcttttttat tgatggttcg tcttttgttt
    15421 gctgtaaccg gtattgacta taattgcccg gaaaatccct gatagcccca tctccttcga
    15481 atacaaataa atggtcaaca agcctgtcca taaaatatcg gtcatgcgaa acaatgagca
    15541 ggcaacccgg aaattcactt aaaaaattct ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa tggagagcag
    15661 gtgcaatctc cttttttccc caccactgag tttactgata taagtgtatt gcttatcagg
    15721 atcgaaaaga aatagctgaa ggaattgtgc tgcagataat gaaccgccgc cggcaagggg
    15781 aaagctttca gccattgatt ttacaaattc aataaccctc atatcttctt ttatgactaa
    15841 cccttgctgg gagtagttgc caaaaattac cgtatctccc acattgattt taccgctgtc
    15901 cgccttttcg attccctgga gcaggttgat aaaagttgac ttccctacac cgttttttcc
    15961 aacaacacct atccgttcgc cctttttaaa agtataatca aatcctttca ggatgatttt
    16021 atcgccataa ctcttgtata cttttttcaa ttctattact ttgccgccca gcctgttcat
    16081 cttcatgtca agctgcattt gttcatcggc cacttgctgt tttgctttct tttcaacttc
    16141 gtagaagtta tcctgcctgc ttttactctt tgtagtcctt gctttgggtt gtttccgcat
    16201 ccattccagc tctttgcggt acaggtttct ggctttgtca atactggcag catcactttc
    16261 tatgcgggct gcttttttat ccaggtaatt ttcataatct cctttataca catacatatt
    16321 actgccatcc agttcccata tctcattgca tactgcatcc aggaagtaac ggtcgtgggt
    16381 caccagcaac aaggttacat tttctttatc aagatagtgc tcaagccatt ctaccatctc
    16441 tacatcaagg tgattggtgg gttcatccat gatcagtaaa acatgtttat ggtcaaaccc
    16501 gatatcaatc aaagtttttg caagtgctac ccttttcctt tgacctccgc tcagcgtatt
    16561 aaccggctgg gaaagatgat gaatatttaa tttaccaagc acctgtttta ctttggtatc
    16621 aaagtcccag gcacccagtt catccatttt aataatagta gagctgagct tctctgcatc
    16681 atttgtttca ctgactgctt catattcttt tattgcatta ataaccggat ggttatggaa
    16741 gaatatgttg tccagaacgg acagctcttc tacaaatgac gggtcttgtt caaaaagtac
    16801 tacatccaca tctttattga tccaaacctt accttcatca gctgtttctt taccagccaa
    16861 tattctgagt aatgtagatt tccctgttcc gtttctggca acaagggcaa ttttatctcc
    16921 ttcttcgata tggaatgaaa tagcagagaa aagtggctga attccgtaac ttttggtcaa
    16981 tccttcaaca gaaacataat gcatgtcgca aatataatct taacggctca ttagccagat
    17041 aagtccgcag atcagcaggg tgaaaaaaag aatgacaagt ggcttatatt tttcaagacg
    17101 atcccttttt tgagtgtttg caacggaatt ttttataagc aatatttcag attcattctg
    17161 atcagccagc ttgaataatt gtctaataac agggtctgac atttctgatg acagcggctg
    17221 tatagttttt aaaaggattg ctataatact tttggtggtt gagtgctcat tgaaggcctt
    17281 tatcttatca ataacgttat cgctaagcat ttccatgagg taatccttca atttttcttt
    17341 atccatcctg aaaacatcaa gccttgaaac gtatttttca agttccaggg attgttttaa
    17401 taacgatacc ggtgtaacga tgccctgcgt tttctgatat cccatacttt ggttatacca
    17461 gcgttgttgc aggtaatact ctttttttgc agggtcagtt aatacttcat aggcttcttt
    17521 tatttcag
//
LOCUS       DAORLS010000026        17420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1747_length_17420_cov_1.978693, whole genome shotgun sequence.
ACCESSION   DAORLS010000026 DAORLS010000000
VERSION     DAORLS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17420
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1747_length_17420_cov_1.978693"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="hisG"
                     /locus_tag="PK977_03615"
     CDS             complement(<1..854)
                     /gene="hisG"
                     /locus_tag="PK977_03615"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRF17224.1"
                     /translation="MIETLKIAIQKSGRLHEGSMKLLKECGIEISNGNNQLRVMAANF
                     PIEVFFLRDDDIPEYVQDAVADIGFVGENVVQEASKSVDAIEKLGFGKCRLSIAAPKN
                     GSINSINDLDGKKIATSYPSILSTYLNEKKISASIHQISGSVEIAPRIGLADAICDLV
                     SSGSTLFTNELKELETIFFSETVMISNKSLSNEKKILLESLLFRIRSVKKAKNNKYVL
                     LNAPNEKLQSICNLLPGMKSPTILPLAEPGWSSVHSVISESDFWNVIEKLKANGAQGI
                     LIIPIEKM"
     gene            1501..2268
                     /locus_tag="PK977_03620"
     CDS             1501..2268
                     /locus_tag="PK977_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRF17225.1"
                     /translation="MLQLKKPLAFIDLETTGINLGTDRIVEIAIVKILTDGTRIVKRK
                     LVNPEMPIPKGASDVHGITDEMVKDAPTFRQVAQELKQVLDGCDIAGYNSNRFDIPLL
                     MEEFLRAQVDFDMKNRKLLDVQNIFHKMEPRTLGAAYKFYCNKNLDGAHSAEVDASAT
                     HEILEAQVSRYPELGNTVESILKTIGEDQIVDFARRFVLENGTEVFNFGKYKGRPVAD
                     VLKSEPQYYDWMMKGDFPQHTKQKLTEIYTRTMLKKG"
     gene            2348..>2619
                     /locus_tag="PK977_03625"
     CDS             2348..>2619
                     /locus_tag="PK977_03625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF17226.1"
                     /translation="MEKLVIIPTYNEKENITSILHAIFNLNEGFHILVIDDGSPDGTA
                     QIVKDLQPKFSGQLFIEERTGKLGLGTAYIHGFKWAISRNYQFIFE"
     assembly_gap    2620..2660
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2661..3174
                     /locus_tag="PK977_03630"
     CDS             <2661..3174
                     /locus_tag="PK977_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRF17227.1"
                     /translation="NPKDLPRLYHACKHQGADVAIGSRYTKGGGVVNWPKNRIALSKG
                     ASFYTRMITWMPVKDPTAGFVCYKKEVLETINLDEIRFVGYAFQIEMKFAAWKLGFKI
                     KEVPIIFQDRTYGTSKMNKGIVKEGILGVLKLRWHSLFTNYRKRVKNSEQLTSVAKEQ
                     KTGEVLAESK"
     gene            3215..4108
                     /locus_tag="PK977_03635"
     CDS             3215..4108
                     /locus_tag="PK977_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF17228.1"
                     /translation="MKRAFLQLHIAVFLAGFTGILGRLITLNEGMIVWYRLLITALTM
                     WLLFGWLKRIQKIPVNDILKITAVGFIAAMHWVTFYGSIKYSNVSVALVCFSAISFFT
                     ALFEPLILKKRINWVELLLGLVTLSGIYIIFHFDTQFKTGIIIGIISAVLASLFPIYN
                     REFLKRINVETMLTWQQTGGFLVLSVLLPFYLQKFPTESFFPNWENIGWLLVLAWFCS
                     VIAFQLSANALKRLSAFTVNLTYNLEPVYGILLAFAVYNENKYLGKGFYIGFAVIAAA
                     LIAHVIILIRVERKLTHDAAE"
     gene            4095..4958
                     /locus_tag="PK977_03640"
     CDS             4095..4958
                     /locus_tag="PK977_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF17229.1"
                     /translation="MQPSEYHQKIIDYYRDTENAYKDSWDLKNSLAIHYGYWDEQVRS
                     FPESLIRMNEVMMKAADIKPSDRVLDAGSGVGGSSIFLASLLGCKVTGITLSERQASH
                     ATENAKKKGVSDLVDFKIMNYTATDFPDASFDVVWGCESICYADDKELFIKEAYRLLK
                     PGGRLVVADGFVSVFENNNNTVIRKWLEGWQVNYLETPERFRQFMNDAGFKDITYQNI
                     SKQASHSSRRLYRFYFLASLYLLWKKINFSRPATEFQKKNIIACKYQYKGLKKWLWQY
                     GLFTGQKLSKD"
     gene            complement(4955..7396)
                     /locus_tag="PK977_03645"
     CDS             complement(4955..7396)
                     /locus_tag="PK977_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF17230.1"
                     /translation="MKKIFTICLGLLFILDTDAQNSGSLTGTVTDKNLLNTLSGVSVE
                     LVGAGKGTMTDSSGKFRITGITPKSYSIRFTSVGYNEVLLYNIVVTSGNEINLSVQME
                     AAITKLEDVVIKSSRRTALAASLETPLSVQRLTTEEIRSNPGGNFDISRVIQALPGVS
                     GTAGSVGGYRNDIIIRGGAPNENVYYLDGIEIPVINHFATQGSAGGPTGILNVSFLED
                     VKLSSSAFDARFDNALSSVFQFRQRNGNPNTFQGNVRLSATELAGTFEGPLSRNTTFL
                     ASARRSYLQLLFKAIDLPIRPSYWDFQYKVTHKLDKKTTLTLIGVGAIDDFTFAAPKD
                     ATPEKLYILDATAPIHQWNYTIGASLRRQIKNGFWNLALSRNMFDNEIEKFDDNDEGN
                     EAKRRLGIFSQEIENKLRFDVNKTIKGWKIAYGAMTQYVKSNNNNFKRLRKELTDQNG
                     NVIQPELAVRYKSDINFWRFGAFAQVSKRYFDDRLGISAGLRTDMNSFTTDGMNPLPA
                     ISPRISVSYVLADKWTLNASVGSYAKIPTYTILSFRNNNGALANEDADYTRSMHYVLG
                     TEFLPNNTTRFTLEGFYKRYSRVPVSVRDGISLANLGGDFGVIGNEDVVSNGDGTTYG
                     FEFFAQQKLTKRFFGTFSYTYFVSKFSGVNGKEISSAWDNRHLLSFIWGYKFNKNWEL
                     GLKFRYQGGAPFTPFDMPASQLNYLTFGTGILDYRQLNTDRLGAFHASDIRIDKKWNF
                     RKITLDLFLDITNWYNAKSPAFPQYTFRRNADNTAFVTTDGQPIQQNGSNAIPFILTN
                     DDASVIPTIGFIIEF"
     gene            7475..>9932
                     /locus_tag="PK977_03650"
     CDS             7475..>9932
                     /locus_tag="PK977_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF17231.1"
                     /translation="MEKKALLFLIYLLPVVFVFAQKKPLDHSVYDGWQRIGERMISND
                     GRWVVYTIDPQEGDNELVIQSSDGQYKKTIARGYNAVISEDSRFAIFRIKPFFKETRE
                     ARIKKKRTDDMPKDSFAIVELGKDSVWKVSKVKGYKVPEKEAGWVAYHLEKTPEKKEQ
                     EKKKPESPDKKIADSLNKVIDSLQQVIQTMPEKKRKKRDGADEEIGFVAEDEKDAEGD
                     EPAGGTSDAGTDLVLRNLTNGQEKVFNNVLEYYFSKTGGKLLMEQAKNPKDSLSKLSV
                     VLYDLNQSKTTVLSKGGNEFKNFSFSEDGSQVAYLAERDARPKELQKFYRIWYHQSGM
                     DSARLLADKNSVGMKLGMTISEYGNLSFSKSGNRLFFGTAPIQPPKDTSLVEMDQVKL
                     DIWHYNDDYLQTVQLFRLQRDLQTNYLAVYHFDRNELHQLGSVDLPTIYPANEGDGDQ
                     FVGVTDYGKRIESQWAGNTKKDIYAINVNDGSRQLVKKDLQGIITPQYISPTGRYIMW
                     YDSKARHYFAWDGTGTRNISQKIKFPLWNEEHDTPNDPPPYGVMGWHEGDSSVYLYDR
                     YDVWKCNMISTFDPLNITGIGRSGKTRIRFVQTDNEARYLKPGENIYYRLFDEKTKSE
                     ELIESTFKDWLSFEQVESGAFTFGQIKKARNADVLLYTRESYTKAPDLYVDKLIPNTI
                     EKAIQLSSINPQQKEYNWGTAELYKWKAFNGKMSEGILYKPEDFDPSKKYPLILYFYE
                     THSNDLHDYIPPTPTGSRLNISFFVSRGYVVFSPDIRYTIGHPAKSAYDYVVSAAQDL
                     AKQKWIDAKNIGIQGQSWGGI"
     assembly_gap    9933..9942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9943..10438
                     /locus_tag="PK977_03655"
     CDS             <9943..10438
                     /locus_tag="PK977_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF17232.1"
                     /translation="LITMTNMFKAAWAGAPVANMTSAYGGIRWESGVNRQFQYEKTQS
                     RIGATLWEKPNLYIENSPLFHLPKVKTPLVIMANDADGAVPWYQGIEMFTAMRRLGKQ
                     VWMLNYNGEAHNLRERKNQKDISIREQQYFDWLLKGEKPPKWIVEGVPAIKKGKDWGL
                     ELVN"
     gene            complement(10506..>11098)
                     /locus_tag="PK977_03660"
     CDS             complement(10506..>11098)
                     /locus_tag="PK977_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17233.1"
                     /translation="LADIIFHYNNIVAFRENKKYLQEHFPKKLTGIQMEKLQILYEQM
                     IEDDELMQELEDIIHYSASRIKRTIHNGTEIYEFVEEKINITPVGLIPLDTQEGYFFL
                     SSGNIKSTRVYHYRLSFFEKHDEKYRSINTVFVDNWVRSISSTYEHIKAELIKQRREL
                     PNPAVYSIETELAFPVDETLLPIAKRSLVKFISEAA"
     assembly_gap    11099..11168
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11308..>11487
                     /locus_tag="PK977_03665"
     CDS             11308..>11487
                     /locus_tag="PK977_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17234.1"
                     /translation="MKVRLLFLLVAFSSCHNTIYVVRHAEKATGIDMATMKPATDPPL
                     SPEGEERALKLKDILG"
     assembly_gap    11488..11497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11498..11837
                     /locus_tag="PK977_03670"
     CDS             <11498..11837
                     /locus_tag="PK977_03670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRF17235.1"
                     /translation="KNVKQIFSTNTLRTISTARPLKELYLGMPIQLYSSKPDSLPSFL
                     KKIRSILKGDVLIVGHSNTVDDIVNGICGDTKIPGDLKDTEYDNLFVLKRKGTGYIFK
                     REKFGKKSPD"
     gene            complement(11834..>12403)
                     /locus_tag="PK977_03675"
     CDS             complement(11834..>12403)
                     /locus_tag="PK977_03675"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRF17236.1"
                     /translation="RSLKYYNDFHYYEVKGGASYAISKGTSVSAGFGIFDTYSPGGNF
                     KTPMVNDEWRTWIQVSLTQYEKRLKLEHRYRAEQRWTSNGFRNRFRYRINTALPLNNK
                     KIEPGTYYLTASNELFFTNRAPYFERTRIFIGGGYEFSHLFTLQAGYLHQFDYRINDE
                     TGKDFFQISLLFELKWKNKSGEKVPGGMD"
     assembly_gap    12404..12415
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12582..13931)
                     /locus_tag="PK977_03680"
     CDS             complement(12582..13931)
                     /locus_tag="PK977_03680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRF17237.1"
                     /translation="MALVDLVMPKLGESIMEATVLKWHKKPGDTVQMDETVLEIATDK
                     VDSEVPSTAAGVIEEILFNVNDVVPIGTTIARIRTGAAEPVASSSPVVLATAQETAKV
                     PMQEAVVVETVPTHSNQKPATGNGSSNRFYSPLVLNIAASEGVSMTELENIPGTGNEG
                     RVTKKDILQYVSGRKSGVVSRESGARPAEMVIPVSKVETDRQTGSNQQLTTYTGNVEI
                     IEMDRMRKLIADHMVRSKQTSPHVTSFTEADVTNLVMWRDKVKKEFEKREGTKITFTP
                     LFIEAIVKCIKKFPLINCSLDGNNIIVKKDINIGMATALPSGNLIVPVIKNADQLNMV
                     GLAKQVNNLADAARNGKLKADDTQGGTFTLTNVGTFGSLMGTPIINQPQVAIMAVGAI
                     KKRPVVIETPAGDSIAIRHMMYLSMSYDHRIVDGSLGATFLTAVAKELENFNIEREF"
     gene            complement(14070..15338)
                     /locus_tag="PK977_03685"
     CDS             complement(14070..15338)
                     /locus_tag="PK977_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRF17238.1"
                     /translation="MTSPSVTASIITIGDELLIGQTIDTNSAFIAQELNKIGVWVRRR
                     VAVGDVYDEIWNALDEESKQSDIIIITGGLGPTADDITKPLLCNYFGGKLVVNEEVLA
                     HVKYLFEKIYRRPGPILERNLKQAEVPDVCTVLHNARGTAPGMLFNKDGRVYISLPGV
                     PHEMKGLMTSEVIPRLLNEFRMPAIVHKTVFTAGQGESVIAELLKDFEPALPAHIKLA
                     YLPNYGMVKLRLTAKGNSKAEVETELQPYFEKLQELVKEYLVTNEDEGLEIVVGKILK
                     EKNKTMATAESCTGGYIAHLITSVAGSSAWYKGSVVSYDNTVKENLLGVQHETLMSAG
                     AVSKETVIQMANGAVQTLKVDYALATSGIMGPGGGSEDKPVGTVWIAVASKEKTETLK
                     LNLRFDRQRNIAMTAANALNFLRKFILSDA"
     assembly_gap    15504..15543
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15544..>15958
                     /locus_tag="PK977_03690"
     CDS             <15544..>15958
                     /locus_tag="PK977_03690"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRF17239.1"
                     /translation="SFYVIDAKTGQVVFDKNSQIGLAPASTQKIITSVTAFELLGKDY
                     RYKTQFLLANIKKEASLLIVPSGDPTLGSWRWNSTRDSNVLANVGMLIKSFGSILNGA
                     VMVDIRKWSSQSTPDGWIWQDIGNYYGAGSGFLNWR"
     assembly_gap    15959..16033
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16034..16804
                     /locus_tag="PK977_03695"
     CDS             <16034..16804
                     /locus_tag="PK977_03695"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRF17240.1"
                     /translation="PTLYLPDFKSEVIAAAKGSGDNAYIYLPVNGANSQVEIRGTIPV
                     NEQSFSISGSMINPPDQFIETVVESNKNNFRQPRYLGSIFLNDSINKQVEREYVHFSP
                     SLDSIIYWFNKKSINLYGEALIKTFAYEKQGFGRTDSGVAIVKKFWKETGLDEDELNI
                     YDGSGLSPLHRVTTHAQVEILKYAKTKDWFPFFLKSLPEYNGMTMKSGTISDVKGFCG
                     YHKAKDGKEYIFSFLVNNYSGKSSQLVGKMYKVLDELK"
     assembly_gap    16968..17116
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17117..17302
                     /locus_tag="PK977_03700"
     CDS             <17117..17302
                     /locus_tag="PK977_03700"
                     /inference="COORDINATES: protein motif:HMM:NF040124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HRF17241.1"
                     /translation="RHFILTGKPLDKQLRMGQIIGLRFASDEEMPALANRAAEEKLSQ
                     KDIKQAIQNWRADYYRV"
BASE COUNT         4896 a         3388 c         3660 g         5069 t
ORIGIN      
        1 atcatctttt caataggtat tataagtatg ccttgagcac catttgcttt taatttttca
       61 atgacgttcc aaaaatcact ctcactgatc acggagtgta cggaactcca gcccggttcc
      121 gcaaggggca gaatagtggg acttttcata cccggaagta agttgcaaat actttgcagt
      181 ttttcattgg gagcattcag taatacatat ttattgttct ttgctttttt aaccgatctt
      241 atcctgaaca aaaggctttc cagtaatatt ttcttttcat ttgagaggct tttgttacta
      301 atcataacgg tttctgaaaa gaagattgtt tctagttctt ttaactcatt ggtaaaaaga
      361 gtgctgcctg aactcaccaa atcacagatg gcatctgcaa ggccaattct tggtgctatt
      421 tctacggagc cacttatctg gtgtattgat gcactgattt ttttctcatt taaataggtt
      481 gacaggatag agggatagct agtagcaatt ttttttccat ccagatcatt aatggaattt
      541 atcgaaccgt ttttaggtgc ggctattgaa agccggcatt tcccaaaacc tagtttctct
      601 attgcatcaa ctgatttact ggcttcctgt actacatttt cacctacaaa acctatatcg
      661 gctacggcat cctgaacata ttcgggaata tcatcatccc gcagaaaaaa tacttcgata
      721 ggaaaattgg ctgccattac cctcagttga ttattcccgt tacttatctc aataccacat
      781 tcttttagca gcttcatgga accttcgtgg aggcggcccg atttctgaat agctattttt
      841 aatgtttcta tcataaaaac ctttgttgca ttacaaataa aaagggctta ctgttgagta
      901 agccctttac tatgttttat acataacatc tcacggccta ccctgaagga gtaagaaatg
      961 atgatgtata tgtgtatata atttcattgc tggtgcaaag ttaggggaat aagacattcg
     1021 gaaaacattt gcatagtttt tcctatgaag ccggtttttt ttacatatta aatgcaacgt
     1081 cttcctggaa cttgtaaaac aagcaacaat taattaataa aaccggcgaa aggctctgct
     1141 aacctctgaa ttttgtgttg gcctgggggt taccggggca accatatttc tgcgaagcac
     1201 aagaagaaat gacaacgatt agactgagta acatcagggc ggctaaaatt ttatttttca
     1261 tatatagtgg atgttatagg tgaataacga aaattgattt caattatttt atatgtgcaa
     1321 agataggaag tgataattaa agaggagtgg taaacctact ggggctttta ccaacataac
     1381 aatctggttg tctttgggtt tacccttaaa atcatgccta aaatagcggc ttaataatac
     1441 ctgtcaatcc attaccttta cggccggtta ttttaaaaaa taagtgaaac tactgaccca
     1501 atgcttcaac tgaaaaagcc ccttgccttt attgaccttg aaacgaccgg tatcaacctg
     1561 ggtaccgaca gaattgtaga aattgccatt gtcaagatac ttacagacgg tacccgtatc
     1621 gtaaagcgaa aactggtaaa cccggaaatg ccgattccca aaggtgccag tgacgtacat
     1681 ggaattacag atgaaatggt gaaggatgcg cctactttca ggcaagtcgc ccaggaattg
     1741 aaacaggtac tggacggatg tgacattgct ggttataact caaatcgttt cgatatacct
     1801 ctgctgatgg aggagttttt gagggcacag gtagattttg acatgaagaa caggaagttg
     1861 cttgatgttc aaaatatttt tcacaaaatg gagccccgta ctttaggtgc agcttataag
     1921 ttttattgca ataagaatct ggatggggcc catagtgcag aagtagatgc atccgctact
     1981 catgaaatat tagaggcaca ggtttcccgc taccctgagt taggcaatac agttgagtca
     2041 atccttaaaa caattgggga agaccagatc gttgattttg cccgtcggtt tgtattggaa
     2101 aacggaactg aagtatttaa tttcggtaaa tacaaaggca ggccggtggc agatgtatta
     2161 aaatctgaac cacaatacta cgattggatg atgaagggtg attttccaca gcataccaag
     2221 caaaaactta ccgagatata tacccgtacg atgttgaaaa agggataatg cgtttttctg
     2281 ccactgacca ttttttaaca tgccatcttt tacgattatc gtaaattagt tgctcagata
     2341 tacttatttg gaaaagttag ttatcatacc gacgtataat gagaaggaaa acattaccag
     2401 tatccttcat gctattttca acctgaatga agggtttcac atacttgtta ttgatgatgg
     2461 ctcaccagat ggtactgcac aaatagtaaa agatctgcag cccaaatttt ccggccagtt
     2521 atttatcgaa gaaagaaccg ggaaactggg tctgggcact gcctatatcc atggatttaa
     2581 atgggctatc agcaggaact atcagttcat attcgaaatn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn caatcccaaa gaccttcccc gcctgtatca tgcctgtaaa
     2701 catcagggtg cagatgtggc cattggttcc cgctatacca aagggggtgg ggtcgtaaac
     2761 tggcctaaaa acaggattgc tttatcaaag ggggcttctt tttatacccg gatgattacc
     2821 tggatgccgg taaaagatcc tactgcaggt tttgtttgtt ataaaaagga agtcttagaa
     2881 accattaacc ttgatgagat ccgttttgtg ggctatgctt tccagattga aatgaaattt
     2941 gcggcatgga aactcggttt taaaataaaa gaagtaccca tcattttcca ggatcgcacc
     3001 tacggaacat ctaaaatgaa taagggtatt gttaaggaag gaatccttgg tgtattgaaa
     3061 cttcgctggc atagcctttt tactaactac cgcaaaagag taaagaactc agaacaactt
     3121 acttccgtag ccaaagaaca aaaaaccggt gaggtgctgg cagagagcaa ataacccatt
     3181 gtttattctc tcttttactt agtttgcagg cctgatgaaa agggcctttc ttcaattaca
     3241 tattgctgtt ttcctggccg gttttacggg tatactgggt cgtcttatta ccctgaatga
     3301 agggatgatt gtttggtaca ggcttttgat tacagctctt actatgtggt tgttatttgg
     3361 ttggctgaaa agaattcaaa aaatacctgt aaacgatatt ctgaaaataa ctgcagtagg
     3421 ttttattgca gcgatgcact gggttacttt ttatggctct atcaagtatt ctaatgtgtc
     3481 agtagcgctg gtttgttttt cagctatcag tttttttacg gccttgtttg agcccctgat
     3541 tttgaagaaa agaatcaatt gggtagaact gctgctgggc ctggttactt tatcaggtat
     3601 ttatatcatt tttcattttg atactcaatt taagaccggg atcattatag gaattatttc
     3661 agctgtcttg gcatccttgt tcccaatcta taacagggaa ttcctgaaac gcattaatgt
     3721 agaaactatg ctaacctggc agcaaactgg tggatttttg gtactgtctg tgttattgcc
     3781 tttttatctt caaaaatttc ctacggaaag ttttttcccc aattgggaaa atataggctg
     3841 gctgctggtg ctggcctggt tttgttctgt tattgccttt caactttcag cgaacgcatt
     3901 gaaacgatta tcggctttta cagttaatct tacttataac ctggagccgg tatatggtat
     3961 tttgctggct tttgctgtgt acaatgagaa caagtatctc ggaaaaggat tttatatagg
     4021 attcgctgtt attgcagcag cgctgatcgc ccatgtgatc atattaatta gagtagaacg
     4081 aaaactgact catgatgcag ccgagtgaat atcatcaaaa aataattgat tattaccgtg
     4141 acacggagaa tgcttacaaa gattcctggg atctgaaaaa cagccttgct attcattatg
     4201 ggtattggga tgagcaggta aggtcatttc ccgaatcact gatacgaatg aatgaagtga
     4261 tgatgaaggc ggccgatata aagccctctg accgggtatt ggatgccggt agcggggtgg
     4321 gaggcagcag catcttcctt gcttccctgc ttggttgcaa agttaccggc attactttga
     4381 gcgaaaggca ggcaagtcat gccacagaaa atgcaaagaa gaaaggagtc agcgacctgg
     4441 ttgattttaa gataatgaat tatacagcca ctgattttcc tgatgccagt tttgatgtgg
     4501 tttggggctg cgaaagtatt tgctatgcag atgataaaga actattcatt aaggaggctt
     4561 accgtttgct gaaaccaggt ggacggctgg tggtggctga tggatttgtt tcagtgtttg
     4621 aaaacaataa caatactgtc atccgaaaat ggctggaagg atggcaggtg aattacctgg
     4681 aaacaccgga aagattcagg cagttcatga atgatgctgg ttttaaagat attacctatc
     4741 agaacatatc taaacaagca tcccactcat caagaagact gtaccgattt tattttctgg
     4801 ctagcctcta tttgctttgg aaaaaaataa atttttcccg gccggctacc gaatttcaaa
     4861 agaaaaatat tattgcctgt aaatatcagt acaagggcct gaaaaaatgg ctttggcaat
     4921 atggtttatt tacaggtcaa aaattatcca aagattaaaa ctctatgatg aaaccgatgg
     4981 tgggtatcac actggcatca tcatttgtca gaataaacgg aatagcgtta gacccatttt
     5041 gctgtattgg ctggccatcg gtggttacaa atgctgtgtt atctgcattc cttctaaatg
     5101 tatattgcgg gaaagcaggg cttttagcat tgtaccagtt ggttatatct aaaaacagat
     5161 cgagggtgat ttttcgaaaa ttccatttct tatcaattcg aatgtcgctg gcatgaaatg
     5221 cgccgagcct gtcagtattc aactgtctgt aatctaaaat accggtacca aaggtcaggt
     5281 agtttaactg ggatgcaggc atatcaaacg gcgtaaaggg ggcgccgccc tggtatctga
     5341 attttaaacc cagttcccag ttcttattga atttataacc ccagatgaac gatagcaggt
     5401 gcctgttatc ccatgcactg gagatttctt taccgtttac accgctgaac ttgctgacga
     5461 aataagtata ggagaaagta ccaaaaaagc gtttagtaag tttttgctga gcaaaaaact
     5521 cgaagccgta agttgttcca tccccattcg aaacaacatc ttcattgccg atgacaccaa
     5581 aatcgccacc caggtttgcc agtgaaatgc catcccttac cgatactggt acccggctgt
     5641 atcgtttata aaaaccctcc agtgtgaagc gggtagtatt gttgggtaaa aattctgtac
     5701 ccaacacata gtgcatgcta cgggtatagt cagcatcctc attagcaagt gcgccgttgt
     5761 tattacggaa gctgagaata gtataggttg gtattttggc ataactgcca actgatgcat
     5821 tcaatgtcca tttatccgcc agcacatagg aaacactgat gcggggtgaa atagcaggaa
     5881 gcggattcat tccgtcagtg gtaaaactgt tcatgtcggt gcgcagcccg gcactgatac
     5941 ctaaacggtc gtcaaaatat cgtttactca cctgtgcaaa agctccaaat ctccagaaat
     6001 taatatcaga tttataacga acggccagtt caggttgtat tacgttgcca ttctgatcgg
     6061 ttaattcttt gcgaagtctt ttgaaattat tgttattgct ttttacgtac tgtgtcattg
     6121 ctccgtatgc aattttccag ccctttatcg tcttgtttac atcaaagcgg agtttatttt
     6181 ctatctcctg tgaaaaaatc ccgaggcgcc ttttggcttc attaccctca tcattatcat
     6241 cgaatttttc aatctcatta tcaaacatat tacggctcaa tgccaggttc caaaacccat
     6301 tttttatttg ccttcttaaa ctggcgccaa tggtgtagtt ccattgatgt atgggagcag
     6361 tagcatccag gatatatagt ttttccggtg tggcatcttt gggtgctgca aaagtaaaat
     6421 cgtcaatggc tcccacaccg atcaatgtca gtgtggtttt cttatccagc ttgtgggtta
     6481 ccttatattg gaagtcccag tagctgggcc gtatgggcag atcaatagct ttaaacaaca
     6541 attgcagata agaccttctt gcagaggcaa gaaaagtagt attcctggat agtggtcctt
     6601 caaaagtacc cgccagttct gtggcgctta accgtacatt tccctggaag gtattgggat
     6661 taccgtttct ctgtctgaat tgaaatacag aagacagggc attatcaaaa cgggcatcaa
     6721 aagcagaaga actcagtttt acatcttcca ggaaacttac atttaatatt cctgttgggc
     6781 ccccggcact tccctgtgtg gcaaagtggt tgatcacagg gatttcaatg ccatcaaggt
     6841 aatatacgtt ttcattgggt gccccgccac ggattatgat gtcatttcga tagccgccca
     6901 cacttccggc tgtaccgctc actcccggta aggcctggat cacccttgaa atatcaaaat
     6961 tcccaccggg attgctgcgt atttcttctg ttgtcagcct ttgtacgctg agaggtgttt
     7021 cgaggctggc tgcaagggct gtgcgacggg aacttttgat aacaacatct tccagtttgg
     7081 taatggctgc ttccatttgt accgaaaggt tgatctcatt tccggatgta acaacgatat
     7141 tgtacaataa gacctcatta tagccaactg aggtaaaacg tattgaatag cttttaggag
     7201 taatacctgt gatacgaaac ttgccggaac tgtctgtcat tgttcctttt ccggctccga
     7261 ccagttctac agatactccg ctcaaagtat tcagcaggtt tttgtccgta acagtaccag
     7321 ttagagaccc ggaattttgt gcatcagtat ctaaaatgaa caaaagaccg aggcaaatag
     7381 taaatatttt cttcatataa gtaaatctat tcaaataaca gccaactaat tgatttgttt
     7441 gctttaactt gttgcccctg attatttaat gcttatggaa aagaaggctc tgttattctt
     7501 gatctacctg ctaccggtag tatttgtttt tgcccaaaaa aagccccttg atcattccgt
     7561 ttatgatggc tggcagcgca tcggcgagcg gatgatcagt aacgacggaa gatgggtggt
     7621 ttataccatc gatccgcagg aaggtgataa cgaactggta atccagtcat cggatgggca
     7681 gtataaaaaa acgatcgcaa gaggctacaa tgcagtgatt tcggaagaca gccgcttcgc
     7741 tattttccgt atcaaacctt tttttaaaga aacgagagag gcaaggataa agaagaaaag
     7801 aacagacgat atgcccaaag attcttttgc aattgtagaa ctgggaaaag acagtgtttg
     7861 gaaagtgtca aaagtgaaag gatataaagt tccagaaaag gaagccgggt gggtagctta
     7921 tcatttggaa aaaaccccgg agaaaaaaga acaggaaaaa aagaaacctg aatcaccgga
     7981 taaaaaaata gctgatagtc tgaataaggt aattgattcg ctgcagcagg tgatacaaac
     8041 tatgcctgag aaaaagagga agaaaagaga tggagcagat gaagaaatag gttttgtggc
     8101 agaagatgaa aaagatgcgg aaggagatga gcccgccggc ggcacttccg atgcaggcac
     8161 tgacctggtg ctacgtaacc ttacaaacgg ccaggaaaaa gtattcaata atgtgttaga
     8221 atactatttc agtaaaacgg gaggcaagtt gctgatggag caagctaaga atcctaaaga
     8281 ttctctcagc aagctatccg ttgttttgta cgatctgaac caatccaaaa caactgtatt
     8341 gagcaaagga gggaatgagt tcaaaaattt tagtttctca gaagatggtt cacaagtggc
     8401 ctacctggca gaacgggatg cccggccaaa agaattgcag aaattctaca gaatctggta
     8461 tcatcaatca ggaatggaca gtgcccgact actggccgat aaaaattcgg tgggtatgaa
     8521 actgggtatg accattagtg agtatggcaa cctgagtttt agcaaatcag gcaatcgcct
     8581 gttttttggt acggctccta tacaacctcc caaggatact tcactggttg aaatggacca
     8641 ggtaaagctg gatatctggc attataatga tgattattta caaacggtac aattattccg
     8701 cttacaaaga gatttgcaaa ccaattacct ggctgtttat cattttgaca gaaatgagtt
     8761 acatcagctt ggttcggttg atctgccaac tatttatcct gccaatgaag gtgacggcga
     8821 ccagtttgtg ggggtgacag attatggcaa gcgtatagaa agccagtggg cgggcaatac
     8881 taaaaaagat atttatgcca ttaatgtaaa tgacggcagc cgtcagttgg tgaaaaagga
     8941 tttacaggga attattacac ctcaatatat ttctcccaca ggcaggtaca ttatgtggta
     9001 cgacagcaag gcccgccact attttgcctg ggatggaact ggtacccgca atatttccca
     9061 gaaaataaaa ttccctcttt ggaatgaaga gcatgatacg ccgaatgatc cccctccata
     9121 tggggtgatg ggatggcatg agggtgattc atctgtatat ctgtacgaca ggtatgatgt
     9181 ttggaaatgc aacatgatat caacgtttga tccgttgaat attacaggta tcgggaggag
     9241 tggaaaaaca agaattaggt ttgtgcaaac agacaatgaa gccagatacc ttaagcccgg
     9301 agagaatatt tattacaggc ttttcgatga gaaaactaaa tcggaagagt taattgaatc
     9361 tacatttaaa gactggttat catttgaaca ggtggaaagt ggggcattta ctttcggcca
     9421 aataaagaaa gccagaaatg ctgatgtttt gttatatacc cgggagtcat atacaaaggc
     9481 cccggattta tatgtcgata aacttattcc caacacaatt gaaaaagcga ttcaactatc
     9541 atctatcaat cctcagcaga aggagtataa ctggggcact gctgaattat acaaatggaa
     9601 agcctttaat ggcaaaatgt cggagggtat tttgtataag ccggaggatt ttgatccttc
     9661 caaaaaatat cctttgatcc tttacttcta tgagacacat tccaatgatc tgcatgatta
     9721 tataccacca acaccaaccg gcagcaggct gaacatttca ttttttgtca gcaggggtta
     9781 tgtggtattc tcaccagata tccgttatac gatcggacat cctgcaaaaa gtgcttatga
     9841 ctatgtggta agtgccgcac aggatcttgc caaacaaaaa tggatcgatg caaagaatat
     9901 tggtatacag gggcaaagct gggggggtat acnnnnnnnn nngctgatca ccatgacgaa
     9961 tatgttcaag gcagcatggg ccggtgcgcc ggtggccaat atgaccagcg cctatggcgg
    10021 tattcgctgg gaaagcgggg taaaccggca gtttcagtat gaaaaaacac aaagccgcat
    10081 tggcgccaca ttatgggaaa aacctaatct ttatatcgag aactctccgt tatttcattt
    10141 gcctaaagta aaaacccctt tggtgatcat ggccaatgat gcagacggag cggtgccctg
    10201 gtaccaggga atagagatgt ttactgccat gcgccgcctt ggcaagcagg tctggatgct
    10261 aaattataat ggcgaggcac ataatctaag ggaaagaaaa aaccagaaag atatatccat
    10321 ccgtgaacaa caatattttg actggcttct aaaaggtgag aaacctccta aatggattgt
    10381 agaaggagtg cctgccataa agaagggcaa ggattgggga cttgaattag ttaattagtc
    10441 aattgtatga ttggttattc aggaaaaccc aaattttcaa tgtcctgatt aaccaattaa
    10501 ctcctctatg cggcttcgct gataaatttt accaggcttc gcttggctat gggtaacagt
    10561 gtttcatcca ccgggaatgc aagttctgtt tctatggagt atacggccgg attgggtagt
    10621 tctctgcgtt gttttatcaa ttcagcttta atgtgttcgt aggtgctgct tatacttcga
    10681 acccagttgt caacaaatac cgtgttaata cttctgtatt tttcatcatg cttttcgaag
    10741 aaagaaagcc tgtaatggta aacccttgtg cttttaatat ttccactgct gagaaagaaa
    10801 taaccttcct gtgtatccag tggtatcagg cctactgggg taatattgat cttctcttcc
    10861 acaaattcat aaatctcggt tccgttatga atagttcttt tgattcggct ggcagagtag
    10921 tgaatgatat cttccagttc ctgcatcaac tcatcgtctt caatcatttg ttcatacagt
    10981 atctgcagtt tttccatctg gataccggtt agctttttag gaaaatgctc ctgcaggtat
    11041 ttcttgtttt cccgaaaagc aacaatatta ttatagtgaa aaataatatc tgccagttnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnntt aactcgaaat ctatacggcc ttcggcaaac caggtttcgg ataaagactt
    11221 catattgcgg attttgattc ctaatttaca aaaaagacga tatgtatgga aaggaagttt
    11281 aattttagaa tgttaaatcg ttaacttatg aaagtaaggt tattgttttt gctggtggca
    11341 ttttcatcct gccataatac tatttatgtc gtaaggcatg cagaaaaagc aacaggaatt
    11401 gatatggcaa ctatgaagcc cgccactgac ccgccccttt ccccggaagg tgaagaaagg
    11461 gcactgaaac tgaaagatat cctcgggnnn nnnnnnncaa aaatgtaaaa caaatttttt
    11521 caacaaatac attgcgtaca atttccactg ccaggccatt aaaagaatta tatcttggca
    11581 tgccgataca gctttacagc agtaagcctg attctttgcc atccttcctg aaaaaaataa
    11641 gatccatcct caagggcgat gtgcttattg tgggtcattc caatactgtt gatgatattg
    11701 tgaacggtat ttgcggggac acaaaaattc ccggtgatct gaaggacaca gaatatgata
    11761 acctgtttgt gctgaaaaga aaagggactg gctatatttt caagcgagaa aaatttggta
    11821 aaaaatcacc ggattaatcc atgccacccg gcactttctc accagattta tttttccatt
    11881 tcagttcgaa caggagagaa atctgaaaaa aatccttccc ggtttcatca tttatcctgt
    11941 agtcaaactg gtgcagataa cctgcttgca atgtaaaaag atgagagaat tcataaccgc
    12001 caccaatgaa aatccgggtt ctttcaaagt agggggccct gtttgtgaag aaaagctcat
    12061 tcgatgcagt tagataataa gttcctggtt cgattttttt attgttcaaa ggaagcgctg
    12121 tatttatcct ataccggaac ctgttgcgaa agccattgct ggtccatctc tgttctgccc
    12181 ggtagcgatg ttctaacttt aaccgttttt catattgggt aaggcttacc tggatccatg
    12241 tcctccattc gtcattaacc attggtgttt taaaatttcc gcccggagag taggtgtcga
    12301 aaattccaaa tccagccgaa acagaggtcc ctttgctaat ggcatagctg gctcctccct
    12361 ttacttcata atagtggaaa tcgttataat actttaatga gcgnnnnnnn nnnnntccat
    12421 ttttgagtaa gatcgagttt ggcgttgacg atattccagc ttcccatttg tgccatgatc
    12481 ggctggatat ataatagcat gatgaaaaaa aatgtatgtc tcatacttaa aagaaaagct
    12541 tctgaatact tcagaagctt taaaaataaa gttctaataa tttaaaactc cctttcgata
    12601 ttaaagtttt ccagttcttt ggcaacagcc gtaaggaaag tggcgccgag actgccatct
    12661 actatccggt ggtcgtagct catgctcagg tacatcatat ggcggattgc tatactgtca
    12721 ccagccggtg tttctatcac aacaggcctt ttcttgatag caccaacagc cataatggct
    12781 acctgcggct gattgataat aggagtcccc atcaggctgc caaaagtgcc tacattggta
    12841 agtgtgaatg tgcctccctg tgtatcatcc gctttcagtt ttccgtttct tgcagcatca
    12901 gccaggttgt ttacctgttt ggccaatccc accatattta gctggtctgc attttttatt
    12961 accggtacaa tcaggttgcc gcttggcagg gcagtggcca tgccaatatt gatatctttt
    13021 ttaacaataa tattattacc atctaacgag caattgatca acggaaactt tttaatacat
    13081 tttactattg cctcaataaa caggggggta aaagttatct tggttccttc tcttttctca
    13141 aattcttttt tcactttgtc ccgccacatt accaggttgg taacatctgc ttcggtgaag
    13201 ctggtaacat ggggactcgt ctgcttgctg cgtaccatgt ggtcagcaat cagtttacgc
    13261 atccggtcca tctcaataat ctctacattt ccggtgtaag tggttagttg ctggttgctg
    13321 cctgtttgtc tgtcagtttc cactttggaa acaggtataa ccatttctgc aggccgggct
    13381 ccggactcac ggcttaccac tcccgacttt cttccgctta catattgtaa aatatctttc
    13441 ttggtcaccc ttccttcatt gccagtgccg ggtatgtttt ccagttcagt catactgaca
    13501 ccttcgctgg cagcaatatt aagaacgaga ggagaataga agcggtttga ggagccatta
    13561 ccggtagcag gcttttggtt ggaatgtgtc ggaactgttt ctacgaccac ggcttcctgc
    13621 ataggcacct tggctgtttc ctgcgcagtg gctagaacga cgggagagga tgaggcaacc
    13681 ggttcggcag cacctgttcg tatacgggca atggtagtgc cgatcggaac tacatcgttg
    13741 acattgaata gaatttcttc aattactccg gctgcagtac ttggcacttc gctgtctact
    13801 ttgtctgttg ctatttccaa aacggtttca tccatttgta cggtatctcc cggcttttta
    13861 tgccatttca atacagtagc ttccataata ctttcaccca gttttggcat tactaaatca
    13921 actaaagcca tataactatt ttagattgca gattttagat tgcagattgt ctggcatacc
    13981 tgccggcagc aataacaatc gttcgggcca aaggtaaagt tttccggcta atcccttaaa
    14041 acatcaatac ctgtctatcg gcaggagaat caggcgtctg acaatatgaa tttgcggaga
    14101 aaattcaagg cattggcagc tgtcatggca atatttctct ggcggtcaaa ccgaaggtta
    14161 agtttgaggg tttcggtttt ttctttgctg gcaacggcga tccagactgt tcccacaggt
    14221 ttatcctcgc tgccaccacc cggccccatg attcctgagg ttgctaatgc ataatctact
    14281 tttaatgttt gaaccgctcc atttgccatt tgtattacgg tctctttact tacagctccc
    14341 gcactcatca atgtttcatg ttgaactccg agcaaatttt cttttaccgt attgtcatag
    14401 cttaccaccg agcctttgta ccaggctgat gagccggcaa cggaggtaat aagatgagct
    14461 atatagccgc cggtacagct ttcggcagta gccattgttt tatttttttc tttgagtatt
    14521 tttccaacta cgatttccag gccttcatcc tcatttgtaa ccaggtattc ttttactaat
    14581 tcctgtagct tttcaaaata aggttgcaat tcagtttcaa cttctgcttt actattacct
    14641 ttcgcagtca gccgcaattt taccatacca taattgggca ggtaagccag tttaatatgt
    14701 gcgggcaagg ccggttcaaa atctttcagc aattcagcga tcacagattc tccctgtcct
    14761 gcggtgaaaa cggttttgtg aacaatggcc ggcatccgga attcgttcaa cagccgggga
    14821 atgacttcac tggtcatcag gcctttcatt tcatgtggta caccaggcaa agagatatat
    14881 acccgcccgt ctttattgaa aagcattccg ggagctgttc ctcttgcatt atgtaaaacc
    14941 gtacacacat ccggtacttc tgcctgttta aggtttcttt ccagtatcgg gccgggtcgc
    15001 cggtatattt tctcgaatag atattttaca tgagccagta cttcttcatt gaccaccagt
    15061 ttgccgccaa aatagttgca gagtaatggt ttggtaatat catcagctgt tggacctagt
    15121 ccgccggtta ttataattat atcggattgt ttactttctt catccaatgc attccatatc
    15181 tcgtcataca catctcccac cgctacccgc cggcgcaccc aaacaccaat tttattcaat
    15241 tcctgggcga taaaagccga gttggtatca atggtctggc cgatcaggag ttcatcgccg
    15301 atagtgataa tggaagcagt tactgagggg gaagtcattt aggaaatgat taatacgaaa
    15361 tgaattagtt ttaagcaaag ttatcaacac atagctatga aaaaagtttt cttgctggtt
    15421 gctggttgtt ttttattggt tgttgtttct tacagtcaga ctgtttccca gcgtttaaac
    15481 aaagcttttg ctgcatttga aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnntcctttt atgtaattga tgcgaaaacc gggcaagtcg tattcgacaa aaactcacag
    15601 attggactgg ccccggcatc cacacaaaag attattacca gtgtgacggc ttttgagttg
    15661 ctggggaagg attacaggta taagactcaa tttttacttg ccaatattaa aaaggaagca
    15721 agccttttaa ttgttccatc aggggaccca acgctgggta gttggcggtg gaatagcaca
    15781 agggatagta atgtcttagc taatgtagga atgcttatca aatcctttgg atcaattttg
    15841 aatggggctg ttatggtaga tattcgtaaa tggagcagtc aatcaacgcc ggatggatgg
    15901 atatggcagg atataggtaa ctactatggc gccggatcag gttttcttaa ttggagagnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnncccactc tttatttacc cgattttaaa tctgaagtga tagctgcagc
    16081 taagggtagt ggtgataatg catacatata tcttccggtt aatggagcca attcacaagt
    16141 tgaaatcagg ggaacaatac cggtaaatga gcaaagtttt tctatttccg gctcaatgat
    16201 taatccgcct gatcaattta ttgaaacagt agttgaatca aataaaaata atttcaggca
    16261 gccacgatat ttaggctcaa tttttttaaa tgattcaata aataaacaag ttgagaggga
    16321 atatgtccat ttttctccct ccctcgattc gatcatctac tggttcaata aaaaaagcat
    16381 taacctttac ggtgaagcat taataaagac ctttgcttac gaaaaacaag gcttcggccg
    16441 tacagacagt ggggtggcta tcgttaagaa attctggaaa gaaacaggcc tggatgaaga
    16501 tgaattgaat atttatgatg gttccggcct ttcacctctc caccgggtta ctacccatgc
    16561 acaagtagaa atattgaaat atgccaaaac aaaggactgg ttcccttttt tcctgaaatc
    16621 attgccggaa tataatggca tgacgatgaa aagcggcact atcagcgatg taaagggttt
    16681 ttgcggttat cacaaggcaa aagatggcaa ggagtatatc ttttcattcc ttgtcaataa
    16741 ttacagtggt aaatcttcgc aactggtggg taaaatgtat aaagtgctgg atgagctaaa
    16801 gtagaccgga gtggttagtc aatagtcctg agtcagggaa gttccctaat ttcggtaaaa
    16861 taacttttat ggctgaacag aattttaaaa accatacccg tcttgtagcc ggttaccatg
    16921 gaataacttt tttgctgata ctttccggcc tcattggcag tatcgttnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnncgtc attttatttt aacaggtaaa ccgctggata agcaacttcg
    17161 tatgggtcaa attattggcc ttcgttttgc cagcgatgaa gaaatgccgg ctcttgccaa
    17221 tagggcggca gaagaaaaat tatcacagaa agatatcaag caggctatcc agaactggcg
    17281 ggctgactat tacagggtgt aataactact cggcggcagc tccccggaaa atcgattcaa
    17341 ctttatcatt cctgaacgta aagatgatgc ctccgtaaat ggagcctaca gttacttgtt
    17401 ctttgttaga ctcttccgca
//
LOCUS       DAORLS010000027        17292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1768_length_17292_cov_2.357545, whole genome shotgun sequence.
ACCESSION   DAORLS010000027 DAORLS010000000
VERSION     DAORLS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17292
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1768_length_17292_cov_2.357545"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..352
                     /locus_tag="PK977_03705"
     CDS             47..352
                     /locus_tag="PK977_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17242.1"
                     /translation="MKKLILAIMSAAIFASCSKEKTSVLSQPAQEKKLKRTERVYNGG
                     TPETTDFTYDSQGRLLTEITNDYLYKYSYPGSNQIIVTKNDKTTNTIKWKSIRKSKS"
     gene            574..843
                     /locus_tag="PK977_03710"
     CDS             574..843
                     /locus_tag="PK977_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17243.1"
                     /translation="MLSSTTVYTVNTSQPYVEIFTQGALFPSPTLFGKLTKNPVVELK
                     TTDASGNVTWHTKTNYEFDAAGYVTKQTINFLHIGTQGQQIYSYE"
     gene            complement(963..3410)
                     /locus_tag="PK977_03715"
     CDS             complement(963..3410)
                     /locus_tag="PK977_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF17244.1"
                     /translation="MRRFMTALVLMATLTAAAQSTQRGTITGKVTDEQNKPLEGITAE
                     LLRSKDSSLVKTALSDKNGQLEFITVPYGDYIIRTSAASYQRTFSGIITLSSSSVAAA
                     DLKLKVAAKELQSVQVTGRKPFIQRLNDRIIVNVENSVVNAGSSAFDVLERSPGVLID
                     PNDNISMRGRAGVIIMIDGKPSPMSGADLVNYLRSLPSNAIDRIELITNPSSKYDAAG
                     NAGIIDIRMKKDQRFGTNGSVSAGYGQGVYPKANGSINFNTRTKKANLFGGYNYAYRM
                     NLNHLILDRNFFTALGVFTGQDKKDNYTKIPLDLHSLRFGADFFPNKKTVLGFVVNSN
                     FNVIRPRNNNSSVVIDENKQPVFNFNTQTTNRNENKNMVANFNIKHTFDSTGKELTAD
                     IDYGYFHADNASRVATQYYNLNGTPMQPDYILDGFQLGKLKLATAKADYVHPLKKNAK
                     LEAGFKTSLVNADNDARFFDMSSGTAVDDVNKTNHFYYDENINAGYLIYKKNFKKWDM
                     QLGLRAENTNVKTRQVKGDIRWDSSYTQLFPSAFFSYKLKENHSLGLSVSRRIDRPNY
                     SQLNPFLFLIDVTTYSTGNPSLLPQFTWSYELSYTMKNINFTLGYSRTTNSQNIVITR
                     FKDVFPNIPADDNITVQIPVNLSSTDYYGLTVAAPIRINKWWNMMNNANIFYNHFNGN
                     LGVTTLDNGKVVADLRTNHTFTLKKGWTAELNANYNSGGQYGFMELRPQWGLGAGVQK
                     SVMKGKGQVRFNVTDIFWTNLPRATITYANYVEIWRAYRETRVANLSFTYRFGSNKIQ
                     QSRRRTTGSEEERQRAQ"
     gene            3650..4000
                     /locus_tag="PK977_03720"
     CDS             3650..4000
                     /locus_tag="PK977_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17245.1"
                     /translation="MKKILATGFLMLLLTACNSDKKKDTGDKGPAVVTGKTEATPIGT
                     YVTKEDGKQMEFTLQADGKGFEIYGTEKRPFTWESRVGKIFFRYDGEATEFELPLDAA
                     KGEIRYGALLYKKQ"
     gene            4049..4468
                     /locus_tag="PK977_03725"
     CDS             4049..4468
                     /locus_tag="PK977_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17246.1"
                     /translation="MIHHSTKWLMMVLLSLAVLAFAIVVLSFRTNKELPQGVVAYTKS
                     VTNITSMTANCNYRITVLDPGKVKGHGVCVSSRDKPLITHMNFPSAAPAQRAADYMVK
                     ITGLKPLSTMYARSYVKVDTGVVYGNVVKFVTLRVEK"
     gene            complement(4477..5322)
                     /locus_tag="PK977_03730"
     CDS             complement(4477..5322)
                     /locus_tag="PK977_03730"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF17247.1"
                     /translation="MESYRKPKPVFYQPAAAVGEKLYFIGIGIDEFRESEYNLKYSVK
                     DIRNLAETFKKKYGSRISIDTLFNSQVTASAVTRLKDKLKQSNENDKVIVAYSGHGLL
                     SKDLDYYLSTYNVSFSNPEENGLPYEEFENLLDNIPARKKLMLIDACHSGEVDKEEML
                     VMNKTADSLGLSKGIIIDQPQQQQHVGLKNSFELMQSLFVNVGKSTGATIISAAAGNQ
                     FALERGDLKNGVFTYSILEAMNKYPSMKISELKKIVGERVQELTNGLQKPTSRNENIS
                     ADWSL"
     gene            complement(7007..>7960)
                     /locus_tag="PK977_03735"
     CDS             complement(7007..>7960)
                     /locus_tag="PK977_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17248.1"
                     /translation="TGARDGQVKLWHTETGKLVYTLPADNYSANAAFAGGGEYIISGG
                     SSSLKGWKTATGQLLYEIKAADFAFMDYHLSPDGKYLAVWKEDKLLIMSVANGKIIDS
                     AAADGSAGETVMAGFAATAPKIFFIRNNKVIERNYLSHADLRQYGSGDDIRSLLADDD
                     KLVIIYYSRTVEVYNHTTGKKLSTAKINLPEFSNLTALDASFILGVKYDRENNIQPLQ
                     LFNTVNGAVIHRFPDSADVYGMPEYCFDKKTGLPVVSLYGSHKKRQYECLQQREPPAG
                     HLQPEGRKGGMANRYSAMGKRICVWQDELHWSARRKQGPLF"
     assembly_gap    7961..7970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8238..>8504)
                     /locus_tag="PK977_03740"
     CDS             complement(8238..>8504)
                     /locus_tag="PK977_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17249.1"
                     /translation="QEVSPWIIYPKEVIFEMSNDGKSWQPLTIIKNKVGTDTKGPVHQ
                     QLGGAVAAKTRYVKVRAVNGGSLPAWHESAGSPSHLFIDEVIVR"
     assembly_gap    8505..8514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8515..>9230)
                     /locus_tag="PK977_03745"
     CDS             complement(<8515..>9230)
                     /locus_tag="PK977_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HRF17250.1"
                     /translation="SGDYALGTPLFDEVKLNLENGKAFTIKTKNFKRGSFYVEDVTLN
                     SKPHPATFIRHSDMANGGELVFTLGDKPNKTWGASENDMPHSRVNDEAFVAVPYFDME
                     SYKFKTTTVVQLKHIDPAAEIYYSLEQEDGSNGKFTRYTKPFTIDKTIQVTIYAEKNN
                     LKSPVTFRKFYQIPADISITVLSEVHPMYTAGGKEALIDGITGNENWKTGDWQSYYGK
                     DFEAIIDLQKEKSLQYVGVHV"
     assembly_gap    9231..9326
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9327..11182)
                     /locus_tag="PK977_03750"
     CDS             complement(<9327..11182)
                     /locus_tag="PK977_03750"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRF17251.1"
                     /translation="MKWLGLMSLFMLVTATTNAQKNYTSLVNPFIGTGGHGHTYPGAS
                     MPFGMIQISPDTRLEGWDGCSGYHYSDSLIYGFSQTHLSGTGIPDYCDVLLMPFTGEV
                     NWKNKDYASPFSHNNEKASPGYYEVVLNKYHIKAAVTSFYRSGIHRYTFDASAKEGKI
                     LLDLQHRDEVLESSVEVINPYEVKGMRRSKSWAQNQVVYFYLKFDQPVKNYGLANNDE
                     LLKLKDITKPSADGKKLKAFFAFELAGNKTVQVKIGISGVSMENARQNLETEIGNADF
                     ATIKQKAEDAWNKELGKIAVSGGTYDQQVAFYTALYHTMLAPNIYTDVNGEYRGTDLK
                     VHKADNFTNYTVFSLWDTHRALHPLHTIINRQRTLDWIKTFLAQYQQGGMLPVWELSG
                     NETFCMIGYHAVPVIVDAYKNGIRDFDTKLALKAMTDYAESNRYGLPAYIKQGFISNE
                     DDHESASKTVEYAYDDWCIAQFARWTGNEEVYKKYLKRCLNYRNLFDPRVKHIRGKVQ
                     GFWYNPFDAAEVNNFFTEGNAWHYSFTAQQDIDGLKKLYGGNEAFAQKLEELFTTQTK
                     LSGREQADVTGLIGQYAHGNEPSHHMAYLFNYAGKPWRTQELVHKICTEFYP"
     assembly_gap    11194..11275
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11276..>11624
                     /locus_tag="PK977_03755"
     CDS             <11276..>11624
                     /locus_tag="PK977_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRF17252.1"
                     /translation="VMKIAVLGAGMVGRAIALDLVKDFLVTSFDVNVANLALLKEKNE
                     RITTINTDLTAYEHYPEWLSVFDLVVTAVPGFMGFKTLEAVINTGKNVVDISFFPEDA
                     LQLDQLAKEKGVTV"
     assembly_gap    11625..11724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11725..12406
                     /locus_tag="PK977_03760"
     CDS             <11725..12406
                     /locus_tag="PK977_03760"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRF17253.1"
                     /translation="VGGLPLHPQPPFNYKAPFSPVDVIQEYIRPARLVENGKIVTKPA
                     LSDRELMDFGKAGKLEAFNTDGLRSLIFTMGHIPDMKEKTLRYPGHIDLIIALQQSGF
                     FDITPVIVNGNNISPLDFTSKLLVNEWKLGETEEEFTVMKVIVKGEGKRITYDLYDQY
                     DPVTQTSSMARTTGYTCTAAVNLIANNIFIQKGVFPPELIGYRKECFDFVLGYLNKRG
                     VQWVKTTS"
     gene            12484..>13630
                     /locus_tag="PK977_03765"
     CDS             12484..>13630
                     /locus_tag="PK977_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRF17254.1"
                     /translation="MRVFLFFILYGITFQVIAQKGNGDPELINAVKAGEINTIKELID
                     KGAAVNAADSNGATALLWAAGIGNSEMVKYFVSKGASLNINGVIYTNTERTSYYGNLT
                     GIAAGEGHLNVLKYLIDSCKISVDDKEYDPESKQKSGWTALQWAASAGKNDVVEYLLD
                     KGADINANHTSDKGTPLLYAIQSGHSQTAELLINKRADVNKANNNNTTPLMAAVEANN
                     ITVCILLWKNKAGLQTKNNEDNTALDIALDKKYYRIIEFLSTPGNYEAVLKKEYWAEL
                     NDSLDFYSNLKEYERAVVVGKRALAAAKKEFGIKHDNYGSTLKSLSVVCRQAGRYELA
                     AKNAEEAAVFYKENAGKTSVAYASILNQLGLNYNALRDTAKSIKNYKL"
     assembly_gap    13631..13643
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13702..>17292
                     /locus_tag="PK977_03770"
     CDS             13702..>17292
                     /locus_tag="PK977_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF17255.1"
                     /translation="MSSFYADNKQYRTALPLLKEMIQLQEEIYGRESADYLDGLSRLA
                     NAYKGSGSIDEALGVRKLKASLSKKMFGDSSVKYAAELNQIAMLYENINDTVNTESYY
                     IQAINILRTHRPLQGLLIDVIENLAGFYEVINRFDDALLYRRENYGYIELLKGANSEL
                     LASNCNQQARLYEELTKYFLAEPLYIQALDIRKKVVGEKHPDYAQSLNNLAGVYEKMN
                     QYEKAEPLYLQAKEIWKTSLGEESRDYLICLNNLGSLYNSMGQYKKAEYYHISSGEIR
                     KKVFGQNSPDYATSLNNLASLYESMGQYDKAEHLYLEAKEIRKKAFGETSNLYAMCLN
                     NLGVLYNTTGKFKKAENFYLESMAIKKNLFGKNHADYANSLNNLAVLYKDMGEYKKAL
                     PFFLECVEIWRKALGEKHSYYATGLNNLATLYSDMGEYEKAEPLYLKAGEVWKKALGE
                     KHPNYATSLSTLGVLYKKIARYDKAELLYLQAQEIFKTAFGEIHPEFANSLNNLAVLY
                     STMGKYDKAEFLYLQSKDIRKMVYGENHPSFATSLNNLAILYEAIGQYEKAEQLYLLS
                     KEIRKKILGGNHPDYALSLNNLAVLYRKLGRFKKAEPLYQESKEIIKKVRGPDHPDYA
                     LSLNNLGALYEVMGENEKSEAAYQEAINIRKKILGENHPDYALSLNNLAYLYTSTGKY
                     DKAEQLYKSSLAKLKKAFGPENTEYALVVNNLASMFMIKGEYFQAEKYFIEYNQIEIK
                     NMLSVFDNLSENEKGNYLEDKLILNDENNSFLYNHKKQNPLLSQTNYNLQLLLKSLSL
                     ASTKTVMEAVRNSGDTSVQILFDKWKANKSILAKQYSLPVIQRRDDIKSIEEQTENQE
                     KELTRKSAEFKNQQQSLEIRTPNVRSRLNEDEIALEFVRFELLNKEWTDSIMYAAYIL
                     TAKDSIPIFVPLCEEKQLQQLFDSAGTTANAMVSKFYRGLELGNAGTAASLGKDLYNL
                     IWAPLEPYLMGIKKISYSPAGKLYSIAFHALPVDSSKLLMDKYELNQYTSTRQVALRK
                     EQETNSKIQSISLFGDARFTMDSTTIAQLKKNNTVSNIYTPPNRGNRGSTWTDLPGTA
                     EEVKKIKQLFEQNQSDRQTGKINTKTFTQNTASEENLKALSGNSPQILHIATHGFFLP
                     EPDKTRKDNFRSNDNSYTLANDPLLRSGLILSGGNYAWSGKAPIEGVEDGI"
BASE COUNT         4926 a         3514 c         3739 g         4802 t
ORIGIN      
        1 acagttttac accgtaatca aaaatcaaaa aacgaaatca acaagtatga aaaaattaat
       61 cttagcaatt atgtcagctg caatttttgc atcctgcagc aaagaaaaga catcagtgtt
      121 gtctcagcca gcacaggaaa aaaaactgaa aaggactgag agagtttata atgggggaac
      181 acctgaaact acagacttta cttacgacag ccagggccgc ctcctcaccg agattaccaa
      241 tgattattta tataaatatt cataccccgg cagtaaccag ataatcgtaa caaaaaatga
      301 taaaactaca aatacaataa aatggaaatc catccgcaaa agcaaatcat agaccattac
      361 atcatacaat aattaactca aatcccttct aaagatgtca atgccattat taaaaatcag
      421 gcaggctcgg tcacaacaac tatcgatttt gcctatgatg cacagggtta tcttaaaggg
      481 gtccaataca catatcccaa cggtacggtt cattattatg agtatacaat tatcaacggt
      541 aattatagta cgaaaaagtt ttatgcagga ggtatgctga gttctactac tgtttacaca
      601 gtgaatacat cacagcctta tgtcgaaata tttacacagg gtgcattatt tccttctcct
      661 accctgtttg gtaagttaac aaagaatcct gttgttgagt taaaaacaac tgatgcatcg
      721 ggtaatgtaa catggcatac aaaaaccaat tatgagtttg atgctgccgg ttatgtgaca
      781 aagcaaacaa tcaatttttt gcacatagga acacaaggcc agcaaattta tagctacgaa
      841 taaattaccc cggtaaggac ctggagcata ataatcttta acagctgcta ttatgctgtc
      901 tccataattg ttaaaaaaac tattgttgtt ctggcaatag ttttttttaa caattgtatt
      961 ttttactgtg ctctttgcct ttcttcttcc gaaccggtgg ttcttcttct cgactgctgg
     1021 atcttgttgc tgccaaaacg ataagtaaag ctgagattgg ctacccttgt ttcccggtaa
     1081 gctctccaga tctctacata gttggcataa gtgatcgtag ccctgggcag gttggtccag
     1141 aaaatatcgg tgacgttgaa gcgtacctgc cccttgcctt tcataacaga tttctgtacc
     1201 ccggcaccaa ggccccactg cggacgcagt tccataaaac catattgccc gccactgttg
     1261 taattggcat tcagctcggc cgtccatcct tttttcaggg tgaaggtatg gttggtccgc
     1321 aggtcagcta ctactttacc attgtccagt gtggtgacgc cgaggttgcc gttgaaatga
     1381 ttatagaaaa tattggcatt gttcatcatg ttccaccatt tgttgatacg aataggcgct
     1441 gctactgtga ggccatagta atcggtggaa gaaaggttga ccggtatctg tacagtgata
     1501 ttgtcatcag ccgggatatt gggaaacaca tctttgaacc gggtgataac gatattctgg
     1561 ctattggtgg tgcggctgta gccgagagtg aagttgatat tcttcatagt ataactgagc
     1621 tcataactcc aggtaaactg tggcagcaga gaagggttgc cggtggagta agtggttaca
     1681 tcaatgagga acaggaacgg gttaagctgt gaatagttgg gcctgtctat ccggcggctt
     1741 accgataaac cgagtgaatg attttctttc agcttataac tgaagaaagc actcgggaac
     1801 aactgggtgt aagaagaatc ccagcggata tcacctttca cctgcctggt ttttacattg
     1861 gtattctccg cccgcaggcc cagctgcata tcccacttct taaaattctt tttgtagatg
     1921 agataaccgg cattgatgtt ttcatcatag taaaaatgat tggtcttgtt cacatcatcc
     1981 actgcagtac cgctgctcat gtcgaagaaa cgggcatcgt tgtcggcgtt taccaaactt
     2041 gttttgaaac cggcttccag ttttgcattc ttttttaaag ggtgtacata atcagctttg
     2101 gcggttgcca gctttagttt acccagctgg aaaccatcga gtatataatc gggttgcata
     2161 ggcgtgccgt tcaggttgta atactgtgtg gccacccttg atgcattgtc ggcatggaaa
     2221 taaccatagt caatatcggc agtcaattct ttgccggtac tgtcgaatgt atgtttgatg
     2281 ttgaagttgg ccaccatatt cttgttctcg ttgcggttgg tggtttgtgt attaaagttg
     2341 aaaacaggct gtttgttctc atcaatcacc actgagctgt tattgttcct cggacggatg
     2401 acattgaagt tgctgttaac cacaaaaccg agcactgttt ttttattggg aaagaaatca
     2461 gcgccgaagc gaaggctgtg caggtcgagc ggaatcttgg tatagttatc ttttttatcc
     2521 tgtccggtaa acacacccag tgctgtaaaa aagttgcggt caagaataag atggttcagg
     2581 ttcatgcggt aggcatagtt gtaaccgcca aagagattgg cctttttggt tcttgtattg
     2641 aaattgatgc tgccgttggc tttgggataa acaccctgtc cgtagccggc ggatacagaa
     2701 ccattggtgc cgaagcgctg gtctttcttc atacggatat caatgatacc ggcattgccc
     2761 gctgcatcat acttgctcga agggttggtg atgagttcga tacggtcaat ggcattggaa
     2821 ggaaggctgc gcaggtaatt caccaggtcg gcaccgctca tgggcgatgg ctttccgtca
     2881 atcatgatga taacaccggc ccggccccgc atactgatat tgtcattggg atcgatcaat
     2941 acaccgggtg aacgttccag tacgtcaaag gcagaactgc cggcatttac cacactgttc
     3001 tctacgttca cgatgatacg gtcattgagc cgttgaataa aaggtttgcg gccggtaacc
     3061 tgtacagatt gtaattcttt ggcagccact tttagtttca ggtcggcagc agccacagag
     3121 gaactgctga gtgtaatgat gccggaaaat gttcgctgat aagaagcggc tgaagtacga
     3181 atgatataat cgccgtaggg tacagtaata aattccagct gaccgttttt atcggagagt
     3241 gctgttttaa ccagtgagga atctttactg cgcaaaagct cagctgtaat gccttccagg
     3301 ggtttgttct gttcatccgt cactttaccg gttatcgttc cccgttgggt gctttgggca
     3361 gcggctgtca gtgttgccat caataccaat gctgtcatga atcgtctcat aaaaataggt
     3421 ttcagcaaaa atggttcttt gacgaattat ttcgtagggc attatgataa gctaacagca
     3481 gggaggataa gtggagttaa gcaacgttaa tgtcttttat gaaaggtgaa tagtgctgat
     3541 gaaaatcata cggataacga ttgcagggat ttggttttgc ctgaacgctg aatctacctt
     3601 tagtaaaggg aatgagtgag tattaaagaa acaaataaaa ataaatgaga tgaaaaagat
     3661 actggctaca ggattcctga tgctgctgct aacggcctgc aacagcgaca agaaaaaaga
     3721 taccggggat aaaggccctg ccgttgttac cgggaaaacg gaagcaacac cgataggtac
     3781 ctacgtcacc aaagaagatg gcaagcaaat ggaatttacc ctgcaggctg atggaaaggg
     3841 atttgaaata tatggtacgg aaaaaaggcc tttcacatgg gagtcaagag tgggcaaaat
     3901 atttttccgc tacgatggag aagccactga atttgagctg ccgctggatg ctgccaaagg
     3961 ggagatccgt tacggggcat tgctttataa gaaacaatag ttgccaatta tttcactgca
     4021 gcattaatgg ctgctttaat acttatatat gatacatcat agcacaaaat ggctgatgat
     4081 ggtgctgttg tcactggcag tattagcctt cgcaatagta gtactgagct tccgcacaaa
     4141 caaggaactt cctcagggag tggtagccta cactaaatca gttaccaata tcaccagcat
     4201 gacggcgaac tgtaattacc gcatcaccgt attggacccg ggcaaagtaa aaggacatgg
     4261 tgtttgtgtt tccagccgtg ataaaccgct gatcacacat atgaactttc cttctgctgc
     4321 gccggcgcaa agggcagcag attatatggt aaagatcacc gggttaaaac ccttatctac
     4381 aatgtatgca cggtcttatg taaaagtgga taccggtgta gtatatggca atgtggtgaa
     4441 gtttgtgaca ttacgggtgg agaaataaat ggatcatcat aaagaccagt ctgccgaaat
     4501 attctcattc cgggaagtgg gtttttgtaa accgttggtg agttcttgta ccctttcacc
     4561 aacgatcttt ttcagttcgc tgatcttcat cgaaggatat ttgttcatgg cttcgagaat
     4621 actgtaagta aagacaccat ttttcaaatc tcctctttcc aatgcaaact ggttgccggc
     4681 agcagcggag ataatagtgg caccggtgct tttacctaca ttgacaaaca gtgactgcat
     4741 caattcaaaa ctgtttttca ggccaacatg ctgctgttgt tggggctgat caataatgat
     4801 accttttgac aagccgagtg aatcagcggt tttattcatg accagcattt cttctttgtc
     4861 aacctcaccg ctgtgacagg catcgatgag catcagtttt tttcttgccg gaatattatc
     4921 cagtagattt tcaaattcct cgtaaggcaa accgttttct tcgggattac tgaagctgac
     4981 attgtaagtt gaaaggtagt agtctaaatc tttgctcaac aggccatgac cggagtaagc
     5041 cacgattacc ttatcatttt cgttgctttg ctttagcttg tctttcagcc tggtgacagc
     5101 ggatgccgtt acctggctat tgaataaagt gtcaatgctg atcctgctgc cgtatttctt
     5161 tttgaatgtt tctgccaggt tgcggatatc cttaacactg tatttcaggt tgtattcact
     5221 ctccctgaat tcatcaatac cgataccgat gaagtacagt ttttcgccga cagctgctgc
     5281 cggctggtaa aataccggtt tgggtttgcg gtagctttcc attccgttgc tgttcattac
     5341 agaggcagta atgttatttt ctcccggtgc cagtgtaatg ctgatggtgg tatccagcgt
     5401 tttgctcttt cggtggcgaa gatcaattcc tttttttcca aagaagggaa catcattgat
     5461 aaacacatgc aggcgatcaa gcaccctgtc ttcatcactg gctttgatcc ggagctgtaa
     5521 acggttgttg ttttgcaaat aggaaatggc agcactgttt gtcagttcgg ctaccggtac
     5581 tttgtaactc tggttgaagg cggctgtatc aatacccagg cgctttatcc ttttatcata
     5641 agcccggcgg taagaacgga tcagggcagt gtcatcattg ccaatatact ccagcacttt
     5701 gtcgggccgg ttgtatttaa tgtcgagctg atcgaaggtg atgatatcgt tctcctttgt
     5761 tacataatgc agcatgcggg cagcgccggg ggtacacata taatagccat ccggtgtacg
     5821 ggtgaaatag cctgcactgt caatggtata aaaagtgtaa agcggcttta atgtgtgggc
     5881 atcccataca aaatgagcac cgggagtaaa actcagcagg tatttttcag gccagctttt
     5941 tccggtattt acagcagaca gctctccggt cagttctttt atttttttgc ccgtatgaat
     6001 atcaaacacc attgtacggc tttcttcttc ggcaatgaac agctcttgtt cattctttcc
     6061 aaagcggatc acaacaggaa aaaaatcaag gttcctgata ctcgaaacgg tttttcctgt
     6121 tttacgatgg atgatgtaaa taacagatcc ctcatccagc tgtggcgctg tataattttt
     6181 ttcagcagtg tgcaccagca ggtaatcgcc gctgctgctg acaagggcca tgattaccgg
     6241 ttgagcaaaa tgctgtttcc caactattcg tttaccggta atatcgatag tagctactga
     6301 gctgtcctgc agcccgattg ttagcaaccg gcggttgttg tcgaagaact gctgtggctt
     6361 atgcagcagg cgggtgcggt aggtgtaacg attactgtca acccggcttt tgattatcag
     6421 ttcacctttg tcattgttca atgccagcaa agaatcatca ccggaaacga tatagttgta
     6481 ccggtacctg aaattattac cctgccatgc ctgatcggcg gcagtattat tttttgttac
     6541 agcaaaagaa tcgagtgaaa cggtaacaaa ctgttcttct tcactgtctg tattgcccag
     6601 caaagcatgg tactgccgtt gagtgtctga aagacggatc atatccatgc tttcttcgct
     6661 aatgactccc ggtgctggcg gccagcggtc ggtattagcg ctgccaacaa tgtattgcag
     6721 cctcagttcc ggaagattcc atacgtaata tcgaggaggc cgtcctgcgt ttctggtata
     6781 tgtcagcaga tgacctgcat ctgctgacat atacattaca ggggagttgt agcctgaaac
     6841 atccctttca aaatttccct tcaactggcc ggtctttggg ttccagatct tgatttgtcc
     6901 gccgtacctt gaatgagtgg ccgctacgga accgtcggca gcaatgatgc tgccggtgat
     6961 attgtaggca gcgccttcca gccagcggct ttcttttgtg gcaagatcaa aataacgggc
     7021 cctgtttccg tcgagcagac caatgtagtt cgtcttgcca aacacaaatc ctttttccca
     7081 tggctgaata ccggttcgcc attcctcctt tccggccttc aggttgtaaa tgaccagctg
     7141 gtggttctct ttgctgtaag cattcatatt gccgtttttt gtgcgagccg tataaggaaa
     7201 caacgggtaa acctgttttt ttatcgaaac aatattccgg catcccgtat acatctgcac
     7261 tgtccgggaa acggtgtata actgctccgt ttacagtatt gaatagttgt aatggctgta
     7321 tgttgttttc ccggtcatac tttactccta aaatgaatga agcatctagg gcagtaagat
     7381 tactgaattc aggaaggttg attttggcgg tggatagttt tttcccggta gtatgattat
     7441 acacttccac tgttctggaa taatagatga ttaccagttt atcatcgtct gccagcagcg
     7501 accggatatc atctccgctt ccatactggc gcagatcagc atgcgacagg taatttcttt
     7561 ctatcacctt attattccgg atgaaaaaga tcttaggggc tgtggcagca aaacctgcca
     7621 tcactgtttc accggccgaa ccatctgctg cagcactgtc aatgatttta ccattggcaa
     7681 cagacattat taataactta tcttctttcc agacagctaa atattttccg tccggcgaca
     7741 aatgataatc catgaaagca aaatcagccg cttttatttc atacaataac tggcctgtgg
     7801 ccgttttcca tcccttcaag gaagaagatc cgccggagat gatatattca ccgcctccgg
     7861 caaaagcagc attggccgaa taattatcgg ccggtaatgt ataaaccagc tttcctgttt
     7921 ccgtatgcca caattttacc tgcccgtccc gggcgccggt nnnnnnnnnn aatataaatc
     7981 tattatcagg actgaattga atactgttaa ccaggctttc attttctttt gcatcgaggt
     8041 gaccggtttg taacaccagt gtgggttcct gggcattgat aataccctgg cagaagaaga
     8101 taaaagaaca aacaataaca attgtattgc gtagcggaga tgacatcatt ggtttttgta
     8161 attcataggg tcaaaaaagc tgaccactgc actaagttcg ttaaactaac atgaaaaaag
     8221 ctaccatgca gagggtatta gcggacgatt acttcatcaa taaataaatg actgggcgag
     8281 ccggcgcttt catgccaggc aggtaatgaa ccgccattta ccgcccttac ttttacatat
     8341 cttgttttgg ccgcaaccgc cccgcccagt tgctggtgaa cagggccttt tgtatctgta
     8401 ccaactttgt ttttaataat cgtcagtggc tgccagcttt tgccgtcgtt gctcatttca
     8461 aaaatcactt ctttgggata aatgatccag gggcttactt cctgnnnnnn nnnnacatgt
     8521 acccccacat actgcaatga tttttctttt tgcaaatcaa tgatcgcttc aaaatctttt
     8581 ccgtagtaac tctgccagtc tccggtcttc cagttttcat ttccggtaat gccgtcaatc
     8641 agcgcttctt tgcccccggc ggtgtacatg gggtgtactt cgcttaaaac agtgatgctg
     8701 atatcagccg gtatttgata gaacttccgg aaggtaaccg ggcttttcag gttgttcttt
     8761 tcggcatata tcgttacctg tatggttttg tcaatcgtaa aaggttttgt gtaccgggta
     8821 aattttccgt tactgccatc ttcctgttcc aggctgtaat aaatttctgc agcgggatca
     8881 atatgtttta gctgaacaac ggtggtagtt ttaaatttat aactctccat gtcgaagtaa
     8941 ggaactgcta caaaggcttc atcatttacc ctgctgtgcg gcatatcatt ttcagatgct
     9001 ccccatgttt tattgggctt atcccccagg gtaaaaacca attcaccacc attggccata
     9061 tcactgtgcc ggataaatgt tgccggatga ggtttgctgt tcagtgttac atcctccaca
     9121 taaaaacttc ctcttttaaa attctttgtt ttaatagtaa atgcttttcc gttttccagg
     9181 ttcagcttca cttcgtcaaa caaaggtgtt cccaacgcat agtctccgct nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnttcg gataaaattc cgtacagatc ttatgtacca
     9361 gctcctgtgt gcgccacggt tttccggcat aattgaataa ataagccatg tggtgactgg
     9421 gctcgttgcc atgtgcatat tggccgatga ggccggtaac atctgcctgt tccctgccgc
     9481 ttagttttgt ttgtgtggta aagagctctt ccagtttttg cgcaaaggct tcattgccgc
     9541 catagagttt ttttaaacca tctatatcct gctgtgccgt aaaagaataa tgccaggcat
     9601 tgccttcggt aaagaaattg tttacttccg ctgcatcaaa gggattatac cagaaaccct
     9661 gcacctttcc cctgatgtgt ttgacacggg gatcaaatag attgcggtag ttcagacagc
     9721 gtttcaggta ttttttatac acttcttcat tgccggtcca ccgggcaaac tgggcaatac
     9781 accagtcatc gtatgcatac tccactgtct tcgatgcgct ttcatgatca tcttcattgc
     9841 tgatgaaacc ttgttttata taagctggca aaccatagcg attactttct gcataatcgg
     9901 tcatggcttt taaagccagt ttagtatcaa aatcacggat accattttta tatgcatcaa
     9961 cgataaccgg tactgcatgg taaccaatca tgcaaaaagt ttcattacca ctgagttccc
    10021 ataccggcag cataccacct tgctggtatt gtgccaggaa tgttttgatc cagtcgaggg
    10081 tacgttgacg gttaatgatg gtgtgcaacg gatgcagggc ccggtgtgta tcccacaatg
    10141 aaaaaacagt gtagttggta aagttgtctg ctttgtgaac cttaagatca gtaccgcggt
    10201 attcaccatt cacatcggta taaatatttg gtgccagcat ggtatgatat aaagctgtat
    10261 aaaaagctac ctgctgatcg taggtgccgc cgctgacagc aattttgccc agttctttat
    10321 tccaggcatc ttctgctttt tgtttgatgg tggcaaagtc agcattaccg atctctgttt
    10381 ccaggttctg ccgggcattt tccatactta cgccggagat accgattttt acctgtacgg
    10441 ttttattgcc tgcaagttca aaggcaaaaa aggctttgag cttttttcca tcggctgagg
    10501 gctttgtgat atcttttagt ttcagcaatt catcattatt ggccagacca taattcttta
    10561 caggctgatc aaatttgagg tagaaataaa ccacctggtt ttgtgcccat gatttacttc
    10621 ggcgcatacc ttttacttcg taaggattga tgacctccac ggatgattcg agtacttcgt
    10681 cccggtgctg caggtcaagc aatatctttc cttccttagc agaagcatca aatgtgtaac
    10741 ggtgtatgcc tgaacggtag aaagaggtta cagcagcttt gatatggtat ttattcaata
    10801 ctacttcata gtaaccggga cttgctttct cattattatg cgaaaaggga gaggcgtaat
    10861 ctttattttt ccaattaact tctccagtaa aaggcatcag caatacatca cagtagtctg
    10921 gtatgccggt accgcttaag tgagtttgag aaaatccgta aatgagagaa tctgaataat
    10981 gataaccgct gcaaccatcc catccttcca gtcttgtatc cgggctgatc tgtatcattc
    11041 caaagggcat gctggccccg ggataagtgt gaccatgtcc gccggtgccg ataaaaggat
    11101 tgactaatga agtataattt ttttgtgcat ttgttgttgc ggtaaccaac atgaacaagc
    11161 tcattaaccc taaccatttc atagtaataa attnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggtg
    11281 atgaagattg cagttttagg tgccggtatg gtgggcaggg ccattgctct tgacctggta
    11341 aaagattttt tagtaacatc ttttgatgta aatgttgcca atcttgcttt attgaaagaa
    11401 aaaaatgaaa ggatcacaac gatcaatacc gaccttaccg cctatgaaca ttatcctgag
    11461 tggctttctg tttttgacct ggtggtgacg gctgttcccg ggttcatggg ttttaaaacc
    11521 ctggaggctg ttatcaatac cggaaagaat gtggttgata tttctttctt cccggaagat
    11581 gcattgcagt tagaccagct ggcgaaggaa aaaggtgtta ccgtnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnntgtcgg tggcctgccg ctgcatccac aaccgccgtt
    11761 taattataaa gcccctttct ctccggtgga tgtgattcag gaatacatcc gcccggcaag
    11821 actggtggag aacggaaaga ttgtaaccaa acccgccctg agcgacaggg agctgatgga
    11881 tttcggcaaa gccggtaagc tggaagcctt taatacagat ggattacgtt cattaatttt
    11941 tacgatgggc catataccag atatgaaaga gaaaacgttg cggtatcccg gtcatattga
    12001 tctcattatt gcgttacagc aatctggttt ctttgatata actccagtaa ttgtgaacgg
    12061 aaataacatt tcaccgcttg attttacatc gaagctgctg gtaaatgaat ggaaactggg
    12121 tgaaacggaa gaagaattta ccgtgatgaa agtgatcgta aaaggtgaag gcaagaggat
    12181 cacttacgac ctgtatgacc agtatgaccc tgtgacccaa acttcttcca tggcccgcac
    12241 caccggttac acctgtacgg cagccgtaaa tcttattgca aacaacattt ttatccaaaa
    12301 aggcgtcttt ccgcctgagc tgattggtta caggaaggag tgttttgatt ttgtgctggg
    12361 gtatttgaat aagcgggggg tgcaatgggt gaaaacaacc agctgatgcg gcagtgcaat
    12421 acccgttcaa gggtatattg taatcagcag cattaattat cttcatttgt aaatactcaa
    12481 cggatgaggg tcttcctctt ttttattctt tacggtatca cttttcaggt tattgcacaa
    12541 aagggtaatg gcgacccgga actgataaat gctgttaagg ccggtgaaat aaatacaata
    12601 aaagaactta ttgacaaagg cgccgccgtg aatgctgcag atagcaacgg ggccacagca
    12661 ttattgtggg ctgcaggtat tggtaattca gagatggtga agtacttcgt cagcaagggg
    12721 gcttctttaa atataaacgg ggtaatttat accaataccg aacggacctc ttattatggc
    12781 aatcttaccg gcattgctgc cggcgaagga catcttaacg ttttgaaata tcttattgat
    12841 agctgcaaaa tatccgtgga tgataaagag tatgaccccg aaagcaaaca gaaaagcgga
    12901 tggacggcct tgcaatgggc agcttctgcc ggaaaaaacg atgtagttga atacctgctg
    12961 gataagggag cagatataaa tgcaaatcac accagcgata aaggaacacc acttctctat
    13021 gccatacaaa gcggccattc acaaacagct gaattattaa taaacaaaag ggctgacgta
    13081 aataaagcca acaataataa caccaccccg ttgatggcag cggtggaagc aaataatatt
    13141 acagtatgta ttttactatg gaaaaataag gcagggttac agacaaaaaa taatgaggac
    13201 aatacggctc ttgatatagc ccttgataaa aaatactacc gtatcattga attcctcagc
    13261 acaccgggca actatgaagc ggtacttaaa aaagagtact gggccgaact aaacgacagt
    13321 ttagatttct atagtaatct aaaagagtat gaaagggctg tagtggtggg aaaaagggcg
    13381 ttggcagcag ccaaaaagga gttcggaata aaacatgata attatggtag tacactaaag
    13441 agtttatcgg ttgtttgccg gcaggccgga agatatgaac tggctgcaaa aaatgcggaa
    13501 gaggcagctg ttttctataa ggaaaatgcc ggtaaaacat ctgttgctta tgcttccata
    13561 cttaatcagc tcggcttgaa ttataatgca cttcgggata cagctaaatc cattaaaaac
    13621 tacaaactgg nnnnnnnnnn nnnggaaatt ctaagactac cgggaaacca ggacaactta
    13681 ctcgaaagtg tgctttacaa tatgtccagc ttttatgcag ataataaaca atatagaact
    13741 gcattgcccc tgttgaagga aatgatacaa ctgcaggaag aaatatatgg acgggaaagt
    13801 gctgattacc tggatggttt aagcaggctg gcaaatgcat ataaaggatc tggaagtatt
    13861 gatgaggctt taggggtaag aaaattaaaa gcatcgttaa gtaaaaaaat gtttggtgac
    13921 agctcggtaa aatatgcggc cgaattaaat cagattgcca tgctgtatga aaatattaac
    13981 gataccgtaa atacagaatc atattacata caggctatta atatactcag gactcatcgt
    14041 ccattgcagg gtctgttaat tgatgtgata gaaaacctgg caggttttta tgaagtaatt
    14101 aatcgatttg atgatgcatt attgtataga agggagaatt acggctatat agaattactg
    14161 aagggagcaa actctgaact tttggcaagt aactgtaatc aacaggccag gctatatgaa
    14221 gaattaacaa agtatttttt agctgaacca ctttatatac aagcccttga cattaggaag
    14281 aaagttgttg gggaaaaaca cccggattat gcccagagcc tgaataatct tgcgggagta
    14341 tatgaaaaga tgaaccagta tgaaaaggct gaaccgcttt atctgcaggc taaagaaatc
    14401 tggaagacat cccttggcga agaaagccgt gattatttaa tatgccttaa caacctcggt
    14461 tctttatata acagcatggg gcaatataaa aaagctgaat actatcatat tagttcaggg
    14521 gaaatcagga aaaaagtgtt tgggcaaaat agccctgatt atgctaccag cctgaacaat
    14581 cttgcttcgt tatatgaatc gatggggcaa tatgataagg ccgaacatct ttacctggaa
    14641 gcaaaggaaa tacggaaaaa ggcttttgga gaaaccagta atctctatgc catgtgcctt
    14701 aataatttgg gtgtcctata caacacaacg ggaaaattta agaaggctga aaatttctat
    14761 cttgaatcta tggcaatcaa aaaaaatctt tttggaaaaa accatgccga ttatgccaac
    14821 agccttaata acctggcagt actctataag gatatgggag agtataaaaa ggcacttcct
    14881 ttttttctcg agtgcgtaga aatatggaga aaagcattgg gtgaaaaaca ttcttattat
    14941 gctaccggtc tcaataatct ggctacactg tattcagaca tgggtgaata cgaaaaagcg
    15001 gaaccacttt acctgaaagc cggagaagta tggaaaaaag cattgggtga aaaacatccc
    15061 aactacgcca ccagcctcag cacacttggt gttttgtata agaaaatagc ccggtatgat
    15121 aaagctgaat tgctgtattt acaagcacag gaaatattta agacggcttt tggagaaata
    15181 caccccgagt ttgcgaatag cctcaataat ctcgctgttt tgtatagcac aatgggtaaa
    15241 tatgataaag cagaattttt atacctgcaa tcaaaagata tcaggaaaat ggtgtatgga
    15301 gaaaatcacc catcatttgc tacaagcctc aataatcttg ctatactata tgaggctata
    15361 ggtcagtatg aaaaagcaga acaactctac ctgctgtcaa aagagatcag gaaaaagata
    15421 ttgggcggaa accaccctga ttatgccctt agtcttaaca atcttgctgt tttatacagg
    15481 aaattaggcc gatttaaaaa ggctgagcca ctttaccagg agtcaaaaga aattattaaa
    15541 aaagtaagag gtcccgatca tcctgattat gccctaagcc ttaacaacct tggtgcttta
    15601 tacgaggtaa tgggcgaaaa tgaaaaatcc gaagcagctt accaggaagc gattaatatt
    15661 agaaagaaaa tactgggaga aaatcatcct gattatgccc taagcctgaa taacctcgct
    15721 tacttatata ctagcacagg taaatatgat aaggccgaac agctttacaa gtcatcactt
    15781 gccaaactaa aaaaagcctt tggcccggaa aataccgaat acgcccttgt tgttaacaac
    15841 cttgcttcta tgtttatgat aaaaggagaa tactttcaag cagaaaaata ttttattgag
    15901 tacaatcaga ttgaaataaa aaacatgctt tcggtatttg ataacctgtc cgaaaatgag
    15961 aagggaaatt acctggagga taaattgatc cttaatgatg aaaacaatag ttttttatac
    16021 aaccacaaaa agcaaaaccc attactttct caaaccaatt ataatcttca actattatta
    16081 aaatcattgt cactggccag tactaaaacg gttatggaag ctgtcagaaa cagcggtgat
    16141 acgtctgtac aaatactatt tgataaatgg aaagcaaaca aatcgatact cgcaaagcaa
    16201 tattcacttc ctgtaattca gcgccgggat gatataaaaa gtatagaaga acaaacagaa
    16261 aatcaggaaa aagaactcac ccgaaaatca gccgagttta aaaaccagca gcaatctttg
    16321 gaaataagaa caccaaatgt aagaagcagg ctgaatgaag acgaaatagc ccttgagttc
    16381 gtacgcttcg aactgctgaa taaagaatgg actgatagca tcatgtatgc cgcttacatt
    16441 ctcactgcaa aagactctat tccaatcttt gttccgttat gcgaggaaaa acaactgcaa
    16501 caactcttcg acagcgccgg caccacggcc aatgccatgg tcagtaaatt ttaccggggg
    16561 ttggagttgg gcaatgccgg taccgccgca tcattgggaa aggacttgta taatttgatc
    16621 tgggctccgc tggagcctta tttaatgggg ataaaaaaga tcagctattc acctgcaggc
    16681 aaattgtata gtatcgcctt tcatgccctg ccagtggata gtagtaaact attgatggat
    16741 aaatatgaac ttaatcaata caccagcacc cggcaggtgg ctttacgaaa agaacaagaa
    16801 acaaatagca aaatacaaag catatcactt ttcggcgatg caaggtttac catggatagt
    16861 acaaccatcg ctcaattaaa aaaaaacaac acggtttcca atatttacac tccccctaac
    16921 cgcggtaatc gtggcagcac atggaccgat ctgcccggta cggctgaaga agtgaaaaag
    16981 atcaaacaat tatttgaaca gaaccagtct gacagacaga caggcaagat caatacaaaa
    17041 acatttacgc aaaacaccgc cagtgaagaa aacctgaaag cactaagtgg caactcaccg
    17101 cagatattgc atatcgctac tcacggtttc tttttaccag agccggataa aacgagaaaa
    17161 gataacttta gaagtaacga taattcctac acattagcta atgatcccct gcttagaagc
    17221 ggcctcattc tttccggcgg caactatgca tggagcggca aggcacccat tgaaggggta
    17281 gaagacggca tt
//
LOCUS       DAORLS010000028        16846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1830_length_16846_cov_1.861354, whole genome shotgun sequence.
ACCESSION   DAORLS010000028 DAORLS010000000
VERSION     DAORLS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16846
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1830_length_16846_cov_1.861354"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>304
                     /locus_tag="PK977_03775"
     CDS             <1..>304
                     /locus_tag="PK977_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF17256.1"
                     /translation="TRTITRPGYITVTNGVDAAFTFTNPTVCHAPALINFTNTSTGPP
                     VLSYQWDFGDGNFSTAASPSHTYLADGSYIVTLVTTSTAGCVDTFRSNPIVIGAFTT"
     assembly_gap    305..374
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <375..>738
                     /locus_tag="PK977_03780"
     CDS             <375..>738
                     /locus_tag="PK977_03780"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF17257.1"
                     /translation="VPVSANWNFGDGGTATGINSNHTYSVPGTYIVRLYNTYSSCVDS
                     AQQTITINPRPVADFTAPATVRCEPPFTVNFQDLSTGGATGWQWDFGDGNTSTLQNPA
                     HTYTSYGSFTVTLVATNG"
     assembly_gap    739..776
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <777..3834
                     /locus_tag="PK977_03785"
     CDS             <777..3834
                     /locus_tag="PK977_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF17258.1"
                     /translation="NCIRIQRANITIPGLPQRGCIPYTTTFNPTITSLDAVTSYNWDF
                     GDGGTSTLPNPTYTYTTQGTYTVRLIITTSTGCTDTLIVPNAIRLGSKPVADFSAAPI
                     PVCGTQQVYFTNLTTPASDEWLWNFGDGGTSTLQNPSHSYSDTGYFTIQLIATNNGCP
                     DTLVRQNYVHVLPPIARFTPVPDCNNRLRFSFTDQSIAPQTWEWDFGDGSPVSTIQNP
                     SHIYPAFGNYTVRLIVTNGACADTTTRSIRTVDESPDFTVNQTTACKTATLNFTATNV
                     NVANLTNYAWDFGDGGIASTGLTTVTHTYTVSGTYTVRLITTDINGCRDTMIKTNYIR
                     INGPLSGFSATNTGGCAGLTTTFNDLSTTDGVNAITNWQWHFGDGNVQDYSAPPFQHT
                     YNTPGVYSVKLVVTDASGCKDSLTRNNLVTATDPVPNFTSADTLTCPGATVRFTNTSA
                     PAGFTSLWDFGDGNTSTTTSPNHVYTATGLYTVKLNITDAFGCPDSLIRNLYIRVDTP
                     GADFSPSDSLSSCTPFRVQFTNTSTFYNSVVWDFGPGQGTSTLNNPVHFYTLPGVYPV
                     KLVVTSPGGCRDSITKNITVSDTAGTRLDYIPISGCSPLQASFNVITPGTISSYFWDF
                     GDGNTTITSTPSITHIYTNYGSFIPRVILEDPSGCLIPLQGLDTFNIIGAKARFGLDD
                     SVFCDRATVNFSDSTTSNDPITSYNWSFGDGNTSTLQNPTHNYSAPGLYTVQLIVQTQ
                     NGCSDTFTRANIIKVVQRPLIDIAGDSVVCINSSLLHSGVFLQPDTSIVTWQWNFPNG
                     NTSTQQNPPAQTYSTAGTFSITTIATNSTGCKDTTLQTVIVNPLPTVTMPPTLTVQNG
                     FPLTIPATYTPNTISWIWSPATGLSCTNCPTPDAGPKFDTFYQVYFTDNNGCSNTGSI
                     MVYVICKNANLFIPNTFSPNGDGSNDVFYPRGRGLERVKLLRIFNRWGEVVFEKRDFP
                     VNDASSGWNGSFKGKTPQPDVYVYQAEVFCENGDIIKLNGNIALVL"
     gene            3842..>4034
                     /locus_tag="PK977_03790"
     CDS             3842..>4034
                     /locus_tag="PK977_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF17259.1"
                     /translation="MKKPILIMCVAFLTAFNKNSASAQDIHFSQFFEAPLLRNPSLAG
                     IFTGDIRVQAVYRDQWNSVT"
     assembly_gap    4035..4046
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4047..4854
                     /locus_tag="PK977_03795"
     CDS             <4047..4854
                     /locus_tag="PK977_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF17260.1"
                     /translation="TGSFNAEQKMPIGKGDDFVTVGIQVLFDKAGTIGWTSTHVLPAL
                     NYHKSLSSDRNRYLSLGFMGGWVQRRFDPSKATTNSQYDNGGLGENFANTQYNYIDGS
                     VGMSYNSNLGNNSDNNYFFGAAYHHFNRPKQTFFYRNAGVELHPKWVFSGGLKLGVTD
                     YAYMTIQGDHSRQGGFTETVAGAMYGLKIGGNPDDPLYTIHAGGFLRWNDAIIPVVKL
                     DYKPFSIAFSYDANISKLKPSSYGRGGFEISISYTGFRKSVGDYILCPKF"
     assembly_gap    4931..5052
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5053..5584)
                     /locus_tag="PK977_03800"
     CDS             complement(<5053..5584)
                     /locus_tag="PK977_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRF17261.1"
                     /translation="MKKLLAEMIGTFSLVLFGCGAAVVSGVAAGGPSGIGLLGISFAF
                     GLAVVAMAYTIGGITGCHINPAITIAMLVAGKIKMGNAVSYIIGQLIGATLAAGVLYF
                     IQKGLPGFEMGEWALGANGWGEGYLGGYNTTSAFIAEAVLTFLFLFVIFGTTSKWGNS
                     TMAGLAIELTLVLIHLV"
     gene            5711..6181
                     /locus_tag="PK977_03805"
     CDS             5711..6181
                     /locus_tag="PK977_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRF17262.1"
                     /translation="MKIEIGQTAPNFTLHDSDKNKVTLSELRGQNVLLLFFPQAFTGV
                     CTKELCGVRDNIALYNNANARVLGISVDSVFTLNKFKEEQHYNFSLLSDFNKEVSALY
                     NSIYHDWILDMKGVSKRSAFVIDKEGTIRYAEVLENAGEVPNFEKIQEVLARLN"
     gene            6315..6659
                     /locus_tag="PK977_03810"
     CDS             6315..6659
                     /locus_tag="PK977_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF17263.1"
                     /translation="MRRILPILSFILLIAITSKGQSSRPIPSDAGDKIVRLYPNPATS
                     YVTFDLPKGYEKSYSIQVFSFLGKKMFESPTATQKTTVDLSDYNRGLYIYHLVETATG
                     KIVESGKFQVSR"
     assembly_gap    6822..6872
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6873..7862)
                     /locus_tag="PK977_03815"
     CDS             complement(<6873..7862)
                     /locus_tag="PK977_03815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRF17264.1"
                     /translation="MTRIFLKRKITPRIANGHPWIFNNEVEKTEGAAEGGEVAEVFTH
                     DQKFVGKGYINPKSQILVRLLTRNKGEEINDNWFLNQIRQCWQYRQQLGYTENCRLVF
                     GEADSLPQLIIDKFNDYFVIQTLALGMDVWKPAIVNAINEIFSPKGIYERNDVPVREL
                     EGLPQQKGFLSVPFDTNIIINENGLRFHVDIENGQKTGYFLDQQDNRRAIQHIVKGAD
                     VLGAFTYTGTFEIHAAHYGAKSVLGLDISANAVAQANKNAELNDVQNHCRFETANAFD
                     VLKQWGKEGKQYDVVMLDPPAFTKSRETIQKAITGYKEINLRGMKLVKPSGFLV"
     gene            8023..8595
                     /locus_tag="PK977_03820"
     CDS             8023..8595
                     /locus_tag="PK977_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRF17265.1"
                     /translation="MAEKETMNAENGSAENQSTIDINTDESLSGTTHLNEPVAEESEL
                     EKLKAELEESKDKYLRMVAEFDNFKRRNAKERIELIQTAGKEVISELLEVLDDCDRAQ
                     KQLEASGDSKAIKEGVMLVFNKLRNTLQARGVKAMETINHEFNADLHEAITEIPAPSE
                     ELKGKVVDEIVKGYYLNDKIIRHAKVVVGK"
     gene            8625..9788
                     /gene="dnaJ"
                     /locus_tag="PK977_03825"
     CDS             8625..9788
                     /gene="dnaJ"
                     /locus_tag="PK977_03825"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRF17266.1"
                     /translation="MSKRDFYEILGVSKGASADEIKKAYRKVAMQYHPDRNPGDKAAE
                     EKFKEAAEAYEVLSDADKKAQYDRYGHAGVSGNGRGGFSGGGMNMEDIFSQFGDIFGD
                     DLFGSFFGGGQRGGRGGQRSRGVRGSNLRVKLKLNYEEIAKGVTKNIKVKKYVGCTTC
                     NGSGAKDKGSVQTCGTCGGSGQVRRVQNTFLGQMQTVTTCPTCNGEGTTVTAKCSACK
                     GEGRVYGEETVTIDIPAGVQEGMQMNVSGRGNAGERGGMPGDLIVLIEEELHKELHRE
                     GLNVAYELHLSFTDAVFGTQVEVPTIDGRAKIKIPAGTQSGKIFRLKGKGFPAVNSYE
                     KGDQLIQVSVWTPQHLSAEEKALLEKLSNSSNFKPQPDKNDKNFFDKIKEMFS"
     gene            complement(9790..>11100)
                     /locus_tag="PK977_03830"
     CDS             complement(9790..>11100)
                     /locus_tag="PK977_03830"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HRF17267.1"
                     /translation="YSNIRRFITLNSLVPPDAVRIEEMLNYFNLSYHEPQGGDLFHIK
                     TTLTACPWNTDNQLYYINLSSKKLNLDTLPPSNLVFLIDVSGSMDMPNRLPLLKSAFR
                     LLVNNLRDKDSVTIVVYGGVTGVMLNCTSGGEKEKILKVIDELEPGGNTPGESGIRIA
                     YREARNHFIKEGNNRVILATDGDFNVGLKTEEELDELITKQKDLGVYLTCLGVGMGNY
                     KDSKIQLLAERGNGNFAYLDNYKEAEKVLLKEFTQTLYAVADNVYMEMDFNPEYVKEY
                     RLVGFDNKVGALRDSLAAIEGGEIGSGYSMLALVEIVPTEINKGAIKDNFTTGKLAEI
                     KLQYQYPNSPKQHLFKYTSHFAYIPFTELDKSYHFSAAVAMFGSFLRNSPFVKEVGWN
                     EIITMAQNSCGANDLLQKEFITLVQQAKTLYSKVKKKKGSGSQW"
     assembly_gap    11101..11122
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11123..11543)
                     /locus_tag="PK977_03835"
     CDS             complement(<11123..11543)
                     /locus_tag="PK977_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17268.1"
                     /translation="MSLSVYAQQFYVRGEVTDEAGNILQNAVILQHKTGYVFRSGSAG
                     TFGILSNQQIDTFSFSMQGYRTERLLVNADNYLRVKMKLLPASATHAHRDKLSSVTKD
                     LEKEVQKTWFTGDETYASLLENNFIAAKLFPSTGLALN"
     assembly_gap    11604..11659
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11660..12189)
                     /gene="hpt"
                     /locus_tag="PK977_03840"
     CDS             complement(<11660..12189)
                     /gene="hpt"
                     /locus_tag="PK977_03840"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRF17269.1"
                     /translation="MGLIKVHDKTFETYLSEDTIQKRVQELAAAINTDYAGKRPMFIA
                     ILNGSFMFASDLFKHLTIEAELCFIKLASYKGLKSSGNVVTSIGLEDDIFNKEIVIVE
                     DIVDTGKTLHNFLPKLEHQQPKSLRIATLLHKSEATEFPLKLDYIGFDIPNKFVVGYG
                     LDYDGLGRNLKEIYQLV"
     gene            complement(12205..>13141)
                     /locus_tag="PK977_03845"
     CDS             complement(12205..>13141)
                     /locus_tag="PK977_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRF17270.1"
                     /translation="TYKDLAIAEMQRTGVPAAITLAQGIHESGAGQGKLVLMSNNHFG
                     IKCKSNWTGESVKHDDDSRGECFRKYKQSEDSYKDHSDFLRNGKHYAFLFQLDPADYT
                     GWAYGLKKAGYATNPKYPQVLIKLIEDYNLQEYTLIALGKNTQATDNIIVKNDLPAEK
                     PAVDEMPQAKKPVYPDGEFKINETKVVFVKKGTPFLAIAKQYDIDLAKIFEFNEITRA
                     QESAKDQLIYLQRKRKTGINDFHIVQPGETLHDIAQLNAIRLESLQEMNWLKENEMPA
                     AGEKLNLKTKATSMPKLAVRENYSLLPSIKTNSTN"
     assembly_gap    13142..13151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13251..13889)
                     /locus_tag="PK977_03850"
     CDS             complement(13251..13889)
                     /locus_tag="PK977_03850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRF17271.1"
                     /translation="MDLIAPLVTQYAEKYSSAENPLLKEISEYTFQHHPEHHMLSGSV
                     QGKLLEMISCMISPDRILEIGTFTGYSAICLAKGLKVQGKLHTIELREEDARVARAYF
                     DQSDYAAQIISHVGNALDIIPGLEENWDLVFIDADKPAYIEYFNLVFPRLRKNGFILA
                     DNILFHGQVLKEEVKGKNAKGIQAFNDFIRERTDIEKVVLTIRDGLYLLRKL"
     gene            complement(13945..>14363)
                     /locus_tag="PK977_03855"
     CDS             complement(13945..>14363)
                     /locus_tag="PK977_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17272.1"
                     /translation="RLFSPGFFVILSTQKTINLKPVIMRKFLLAVLLPVSVFILIACG
                     GGEKKADVKVSTEMQDFMNNFNGRSASVKIALDDFGKPGLDRKDMDLYNLEKPKVLEA
                     SGNCYLMECKSGMTKRKYNICWEGGKISSIEDKGME"
     assembly_gap    14364..14381
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14382..>14993)
                     /locus_tag="PK977_03860"
     CDS             complement(<14382..>14993)
                     /locus_tag="PK977_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17273.1"
                     /translation="TKGIAVPPDDLFPTIALSDAFSGTIGIIFIIALISALFPSVDGA
                     ITSLTSCFCIDILGLNKKEGTERHKKNTRLKVHFTFALVFFIMVLIFKWINDKLIIDF
                     ILKFAGITYGPLLGLFAFGILTKRKLKENLIWAVCIIAPLLALSVDILSNPAWYEAKL
                     HVKLGLQYISESIFNGYKIGNELILINGIFTFLGLWMISSKHSE"
     assembly_gap    14994..15012
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15013..>15353)
                     /locus_tag="PK977_03865"
     CDS             complement(<15013..>15353)
                     /locus_tag="PK977_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF17274.1"
                     /translation="TDTLQTTGMLVGLVACIIVIVKALGTDFGGALSMMSEKGYTQIF
                     NWDVKAPSYIWKHIIGGMFIAIAMTGLDQEMMQKNISVKTLKDSQKNMMTFTTVLMIV
                     NFLFLVLGGVL"
     assembly_gap    15354..15385
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15386..15900)
                     /locus_tag="PK977_03870"
     CDS             complement(<15386..15900)
                     /locus_tag="PK977_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF17275.1"
                     /translation="MSPAVLFAFVIGYFVLLLVVAWYTSRNSNNESFFIGNRNSNWML
                     VAFGMIGTSLSGVTFVSVPGSVGTSNFYYYQIVLGYLLGYMVIAFVLLPLYYRMNLTS
                     IYTYLEKRFGINAHKAGAFFFILSRTVGATARLYLVISVLQIFIFNKLGIPFEATALV
                     ILVLILLYTFEG"
     gene            16004..16543
                     /locus_tag="PK977_03875"
     CDS             16004..16543
                     /locus_tag="PK977_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRF17276.1"
                     /translation="MAITSLAVFCGSKDGINPIFSEHARTLGKLMAEYNVRLVYGGGS
                     AGIMGIIADSVMQHGGKVTGFIPRLLLEWEVQHRGITELIICDDMHERKKRIYSISDA
                     AIILPGGFGTLDELFEIVTWNQLTIHDKEIYILNSVGFYNHLIEHIEVMKQEQFLYEE
                     ALKRITVLDDPSELITYLK"
     gene            complement(16547..>16846)
                     /locus_tag="PK977_03880"
     CDS             complement(16547..>16846)
                     /locus_tag="PK977_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17277.1"
                     /translation="EHNFISLRYVPATSNYQPYKETVDANSFLVGGGYCQGRQAGSNT
                     FYYVSVLFDVIKNENSPYVDVVYDQVTGRRSVRAVPLIRAGINIGLFQGRNRRFE"
BASE COUNT         4906 a         3558 c         3210 g         4722 t
ORIGIN      
        1 acccgaacta ttacccgccc aggctatatt acagttacaa atggtgtaga tgctgcattt
       61 acttttacca atcccactgt ttgtcatgcg cctgctctta tcaattttac caatacttcc
      121 actggaccac ctgtattaag ttaccaatgg gattttggag atggtaattt ctcaaccgct
      181 gccagccctt cccatacata cctggcagat ggtagttata ttgtgacact ggtgactacc
      241 agcaccgctg gttgtgtgga cacattccga agtaatccga tagtgattgg cgcatttact
      301 acttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnncggttc cagtttctgc caactggaat tttggtgatg gtgggactgc
      421 tacaggtatt aacagtaatc atacttactc agtgcccggc acctacattg tccgtttata
      481 caatacatat tccagttgtg tcgactcagc ccaacagacc attactatca acccgaggcc
      541 agttgctgat tttaccgcac cagcaactgt ccgttgcgaa cctcccttca ctgtcaactt
      601 ccaggatctc tcaaccgggg gagcaactgg ctggcaatgg gatttcggag acggaaatac
      661 ttctacccta caaaacccgg cacatacata taccagttat ggaagcttca ctgtaacact
      721 tgtagcgaca aacggtttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaac
      781 tgtatccgga tacagcgggc caatatcact atacctggtt taccacagag aggctgtatt
      841 ccgtatacta ctacctttaa tccaactatt acatcattag atgcagtcac ttcttataat
      901 tgggattttg gcgatggcgg cacctccaca cttccaaacc ctacttatac atacacaact
      961 caaggcacat ataccgtaag attgattatt acaaccagta ctggttgtac cgacacatta
     1021 attgttccca atgctatcag gctcggcagc aaacccgttg cagatttttc tgcagcgcct
     1081 atacctgttt gcggcactca acaggtgtat ttcactaacc ttaccacacc agcgtcagat
     1141 gaatggctct ggaattttgg cgatggcggt acttccactt tgcagaaccc atctcacagt
     1201 tatagtgata caggctactt caccatacaa ttaattgcta caaataatgg ctgtcccgat
     1261 acacttgtca ggcagaacta tgttcatgtg ctgcccccaa ttgcccggtt tacgccagtg
     1321 cccgattgta ataatcgtct gcgattcagt ttcacagatc aatccatagc gccacagaca
     1381 tgggaatggg actttggtga tggatcacct gtttctacta ttcaaaaccc ttcacatatt
     1441 tacccggcct ttggcaatta taccgtacga ctaattgtaa caaacggagc ctgtgccgat
     1501 accacaaccc gatctatcag gacagtagat gaaagccctg attttactgt taaccaaaca
     1561 acagcctgta aaaccgctac attaaatttt acagcgacaa atgtgaatgt tgccaatctt
     1621 accaattatg cctgggattt tggcgatggt ggaatagctt ctacaggttt aacaactgta
     1681 acacacactt acacagtttc aggaacatat acagtaagat taatcacaac tgatattaat
     1741 ggttgccggg ataccatgat caaaacgaat tatatccgta tcaatgggcc gttatcaggt
     1801 tttagtgcaa ccaacaccgg aggctgtgca ggtttgacaa ccacgtttaa tgatctttcc
     1861 accacagatg gtgtaaatgc aataaccaac tggcaatggc attttggaga tggcaatgtg
     1921 caggattaca gtgcccctcc gtttcagcat acctataaca cacccggagt gtattctgta
     1981 aaactggttg taactgatgc ttccggttgt aaagacagtc ttacaagaaa caaccttgta
     2041 actgcaacag accctgtacc caattttaca agtgccgaca cattaacttg tcccggtgct
     2101 acagtgcgtt ttaccaacac ttctgcacca gccggtttta caagtctttg ggattttggt
     2161 gatggcaata catctaccac aacctctcca aatcatgtgt acaccgcaac aggactatat
     2221 actgtcaagc ttaatataac cgatgccttt ggttgtcccg attcactaat ccgaaattta
     2281 tatatccgtg tagatacacc aggggctgat ttttcaccca gtgattcgtt gagttcctgt
     2341 actccattcc gggttcagtt tacgaatacc tcaactttct acaattctgt agtttgggat
     2401 tttggaccag gacagggaac atcaacactt aataatcctg tacattttta cacacttccg
     2461 ggagtatatc cggtgaagtt agtggtaaca agcccgggcg ggtgcagaga ttccattact
     2521 aaaaatataa ccgtttctga tactgccggc acaaggctgg attatatacc aattagtggt
     2581 tgtagtcctt tgcaggccag ctttaatgta ataactcctg gcacaatatc ttcttatttc
     2641 tgggactttg gtgatggaaa taccactatt acttctacac cttctattac gcacatatat
     2701 actaactatg gttccttcat tccaagagta atattggaag acccttccgg ttgcctgata
     2761 ccgctgcaag gacttgatac ttttaatatc attggcgcaa aagcaagatt tggtcttgac
     2821 gactcagtat tttgcgacag ggcaactgtg aatttttctg attccacaac ttcgaatgat
     2881 cccattacaa gttacaactg gtcttttgga gatggcaata cttctacttt gcaaaacccg
     2941 actcataatt attcggcacc gggattgtat acagttcaat taattgtaca gacgcaaaat
     3001 ggctgttcgg atacatttac ccgggcaaat attatcaaag tagtacagcg gccactaata
     3061 gatattgcag gtgattcagt tgtctgtatc aactcatctc tattacattc cggagtattc
     3121 ctgcagcctg acacctccat tgtcacctgg caatggaatt ttcctaatgg aaatacttcc
     3181 acacagcaaa atccaccggc gcagacttac tctacagcag gcacattttc tatcaccacc
     3241 attgctacaa acagcaccgg atgtaaggat actacgttgc aaactgtgat cgtaaatcct
     3301 ttaccaactg ttacaatgcc accaacattg acagtgcaaa atggttttcc actaacgata
     3361 cctgctacat atacacccaa cactattagt tggatttggt caccggctac aggtttgagc
     3421 tgtactaatt gtcccacacc tgatgcagga cctaaatttg acacttttta ccaggtatac
     3481 tttacggata ataatggctg cagtaataca ggctctataa tggtttatgt gatctgtaag
     3541 aatgccaact tgtttattcc aaatactttc tccccgaacg gagacggcag taacgatgtg
     3601 ttctatccac ggggaagggg acttgaaaga gtgaagcttc tacgcatctt caacagatgg
     3661 ggtgaagtag tatttgaaaa aagagacttc ccggtaaatg atgcgtcatc cgggtggaac
     3721 ggaagcttta agggaaaaac tccgcaacca gatgtttatg tttaccaggc tgaagttttt
     3781 tgtgaaaacg gagatataat taaacttaat ggcaatatag cccttgtact ctgatatgaa
     3841 aatgaaaaag ccgatattga taatgtgtgt tgctttcctg actgcgttta ataaaaacag
     3901 cgcatctgca caggatattc atttctctca attctttgaa gcgccacttt tgcgtaatcc
     3961 ttcattggcc ggcatcttca caggcgacat acgggtacag gcagtttaca gggatcagtg
     4021 gaatagtgtg accannnnnn nnnnnntacg ggttcgttta atgcagaaca aaaaatgcca
     4081 attggcaaag gagacgattt tgtaactgtc ggtatacagg ttttatttga taaagcagga
     4141 accatcggat ggacatcaac ccatgtattg cctgcattga actatcacaa atcgttaagc
     4201 agtgaccgta acaggtattt gtcattagga ttcatgggcg gctgggtgca gcggagattt
     4261 gacccatcca aagccactac gaatagtcaa tatgataatg gtggactggg agagaatttt
     4321 gccaacacac aatacaacta tattgacgga agtgtgggaa tgagttataa ctcaaacctg
     4381 ggaaataatt ccgataacaa ctatttcttt ggcgcagcat accatcattt caaccggccg
     4441 aaacagactt ttttttaccg aaatgcaggt gtggaattac atcctaaatg ggttttctcc
     4501 ggcggattga aattaggtgt taccgactat gcttatatga ccattcaggg agatcatagc
     4561 cgacagggag ggttcacaga aacagttgcc ggtgctatgt atggccttaa aataggcggt
     4621 aatcctgacg acccgctgta caccattcat gcaggaggtt tcctacgctg gaatgatgcg
     4681 atcatacccg ttgttaagct ggattataag cccttctcta ttgcattcag ctacgatgcg
     4741 aatatttcga aactaaaacc ttccagctat ggccggggtg gttttgagat ctccatttct
     4801 tacacaggct tccgcaaatc agtcggcgat tatattcttt gcccaaaatt ttagaacagt
     4861 acaaaatttt ttaagccggt tcttttgtct caaacagccc tttccaaaca aaagctgcta
     4921 taattgcgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nncaaccaga tgaatcaata ccaatgtaag ttcaattgcc agtcctgcca
     5101 ttgtactatt accccatttc gatgttgttc cgaaaatgac aaaaagaaac aggaaagtca
     5161 atactgcttc tgcgatgaaa gccgaggttg tattataccc tcccaggtat ccttcacccc
     5221 agccgttggc acctaatgcc cattcaccca tttcaaaacc tggcaatccc ttttgtataa
     5281 aatataatac acctgcagcg agagtggccc cgattaactg gccaataata taactaactg
     5341 cattacccat tttgatcttg ccggctacaa gcattgctat ggttatagca gggttgatat
     5401 gacaacctgt aattccccct atcgtatagg ccatcgccac aacagctaat ccaaacgcaa
     5461 aagaaatacc caataatcct ataccggaag gaccacctgc tgcaacacct gatacaacag
     5521 ctgctccgca accaaaaagc actaatgaga aagtgccgat catttcagct aataattttt
     5581 tcataaaaag tgatttaagt gaagaattaa acaacctaat caacttttca aagggatgat
     5641 tgtatcactg aaggcaagtc gaatatactt aaataaattc gccattttaa taactaaaac
     5701 aggataacaa atgaaaattg agatcggaca aacagccccc aattttacct tacatgacag
     5761 cgacaagaat aaagtgaccc tatcggaact aaggggacaa aatgtgttac tgcttttttt
     5821 cccacaggca tttacgggtg tatgtaccaa agaactctgt ggagtgagag ataacattgc
     5881 cttgtacaat aatgccaatg ctagagttct gggcatttct gttgattcag tctttacact
     5941 caacaaattc aaagaagaac aacactacaa tttttccctg cttagtgact tcaataaaga
     6001 agtttcagca ttatataaca gcatttacca cgactggatt cttgatatga agggagtatc
     6061 caaacgttca gcttttgtta tagataaaga aggaaccata cgatatgcgg aagtattaga
     6121 aaatgccgga gaagtcccca actttgaaaa aattcaggaa gtgctggcca ggctcaacta
     6181 aattattcgt taaaagtttt gtcaaacaga ttgtaaatct atgatttaca atctgtttta
     6241 atttatcgta gttttacgtt gctcaattaa aattttcggc ttacttttcg aataataaaa
     6301 gtaaatttgt ttcgttgaga cgaattctac ctatactgtc ctttattctg ttgattgcca
     6361 ttacctcaaa aggccaatca tccagaccta tcccttctga tgcaggggat aagattgtgc
     6421 ggttatatcc caacccggct acatcttatg taacatttga cttacctaaa gggtatgaaa
     6481 aatcatactc gattcaggta tttagctttt taggtaagaa aatgtttgaa agtcccacag
     6541 ccacgcaaaa aactactgtt gatctgagtg attacaatcg aggactttat atctatcact
     6601 tagtagaaac tgcgactggt aaaattgtcg aatccggcaa attccaggtt tcccgttaaa
     6661 cagataattc tgtcttttat tactgaacct gtacaatcag gaacttaagg tattgtgtat
     6721 tatccattcc ccggataatt ggatgatctc ctgcctgtgt ttgaaaagta acctgtctta
     6781 attttcttct ggcatctttt gctgccatat caatgatttg annnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaccagga aaccgctcgg ttttaccagt
     6901 ttcatacccc gcagatttat ttctttataa ccggttattg ctttctgtat ggtttccctg
     6961 ctttttgtaa aggcaggcgg atcaagcatg acaacatcat attgttttcc ttcttttccc
     7021 cattgcttta aaacatcaaa agcattggca gtttcaaacc ggcaatgatt ttgtacatca
     7081 ttcagctcag catttttatt ggcttgtgct acagcattag ctgaaatatc aagtcccagt
     7141 acagacttag ctccataatg tgcagcatga atttcaaatg tacctgtata cgtaaatgcc
     7201 cccagaacat ctgccccttt tacaatatgc tgaatagccc ttcggttgtc ctgctgatct
     7261 aaaaagtatc cggtcttttg cccgttttca atatctacat gaaaacgcaa gccattttca
     7321 ttaatgataa tgttggtgtc aaaagggact gacaaaaaac ccttttgctg gggcaaccct
     7381 tctaattccc tcaccggtac atcatttctt tcataaatac ctttcggaga aaatatttca
     7441 ttgatagcat ttacaatagc aggcttccat acatccattc ccagcgctaa ggtctgaatt
     7501 acaaaatagt cattgaattt gtcaatgatc agttgaggca aagaatctgc ttctccaaat
     7561 acaagacggc aattttcagt atagcctaac tgctgccggt attgccagca ttgcctgatc
     7621 tggttcagaa accagttgtc atttatctct tcgcctttat ttctggtaag caggcgaacc
     7681 agtatttgcg attttgggtt gatatacccc tttccaacaa acttttgatc atgagtaaaa
     7741 acctctgcta cctctccacc ctctgctgca ccctctgttt tttccacttc attattgaaa
     7801 atccaggggt gaccattggc tatccggggg gttatctttc gtttcaggaa aattcttgtc
     7861 ataaagggct gcaaggtact ataataaaac aaggtcagtg ccagcaatca acatcttctg
     7921 ccatacattc aaggaaatgc agcaaggacg gtcattttgt ccagaaaacc gctgttggca
     7981 atattcttga cgaccttacc tttgcaatca attttgaaaa aaatggcaga aaaggaaacg
     8041 atgaatgcag aaaacggaag tgctgaaaat caaagcacta ttgacatcaa tacggacgaa
     8101 agcctaagcg gtactaccca cctcaatgaa ccagtagccg aagagtcaga actggaaaaa
     8161 ttaaaagcag agttggagga atctaaggac aaatacctgc gaatggttgc tgagtttgat
     8221 aattttaaac gtcgtaatgc caaggaacga attgagctta ttcagacagc aggtaaagag
     8281 gtgatttctg aactgcttga agtcctggac gattgcgaca gggctcaaaa acagctggag
     8341 gcaagtggtg acagtaaagc aataaaggag ggtgtaatgc tggtattcaa taagcttcgc
     8401 aatacccttc aggcaagagg tgtaaaagca atggaaacta tcaaccatga attcaatgcc
     8461 gatcttcatg aggctattac cgaaatacct gccccatccg aagaactaaa aggcaaagtg
     8521 gtggatgaaa ttgtaaaagg ttattatcta aatgataaaa tcatacgtca cgccaaagtg
     8581 gttgtaggaa aatagttttt gataaaatag ttaataaaaa gaggatgtct aaaagggatt
     8641 tttacgaaat actaggtgta agcaaaggcg cttctgcgga tgaaataaag aaagcatacc
     8701 gtaaggtagc catgcaatac cacccagaca gaaatcccgg tgataaagct gctgaagaaa
     8761 aattcaagga ggctgcagag gcttatgagg tactgagtga tgcagataaa aaagcccaat
     8821 acgaccgtta cggccatgca ggcgtaagtg gtaatggccg gggaggcttt agtggtggcg
     8881 gtatgaatat ggaagatatc ttcagtcagt ttggagatat ttttggcgat gacctctttg
     8941 gaagtttttt cggtggcgga caaagaggag gccggggagg ccagcgtagt cgtggtgtta
     9001 ggggtagcaa tcttagggtt aaactaaagc tgaattatga agagattgcc aaaggcgtaa
     9061 ccaagaatat taaagtaaaa aaatacgttg gttgtaccac ttgtaatgga agcggggcaa
     9121 aagacaaggg tagtgttcaa acctgtggga cctgcggtgg cagtggccag gtaagaaggg
     9181 ttcaaaatac ttttctcggt caaatgcaga cggtaaccac ctgccccacc tgcaatggtg
     9241 aaggcactac cgttaccgca aaatgttcag catgtaaggg tgaaggcagg gtttacggag
     9301 aagaaaccgt aaccattgac atacctgccg gtgtacagga aggcatgcaa atgaacgtaa
     9361 gcggcagggg taatgccgga gaaagaggag gaatgcccgg tgacctgatt gtcctcattg
     9421 aagaagaact acacaaagaa ttgcaccggg aaggattaaa tgtagcctat gaactgcatc
     9481 tttcttttac cgatgctgta ttcggcacgc aggtagaagt tcctactatt gacggaagag
     9541 caaagattaa aataccagct ggcactcaaa gtggaaaaat tttccggctg aagggcaaag
     9601 gttttcctgc cgtaaactct tacgagaaag gcgaccaact catacaagta agtgtatgga
     9661 caccgcagca tttaagcgct gaagaaaagg cattgctgga gaaactaagc aactcctcta
     9721 acttcaaacc gcaaccagat aagaatgata aaaacttttt tgataagata aaggagatgt
     9781 tttcttaatc taccattgtg agccactccc ttttttcttc tttaccttag agtacaatgt
     9841 ttttgcctgt tgcaccaggg taatgaattc tttttgtaac agatcgtttg caccacatga
     9901 attttgtgcc attgtaatga tctcattcca tcctacttct tttacaaaag gtgagttgcg
     9961 gagaaaagaa ccaaacattg ctaccgctgc tgaaaaatgg tagcttttat ctaattcggt
    10021 gaagggtata tatgcaaaat gactggtata cttaaataaa tgctgtttgg gactattggg
    10081 atattgatat tgtaatttta tctccgctaa ctttccagta gtaaaattat ccttaatggc
    10141 gcctttgttg atctccgttg gaacaatctc aaccagggcc agcatggaat agccggaacc
    10201 aatctcaccg ccttcaattg cagccaagga atcccgcaaa gccccgactt tattgtcaaa
    10261 ccccaccagc ctgtattcct taacatattc cgggttaaaa tccatttcca tataaacatt
    10321 atcagctacg gcatatagtg tttgagtaaa ttccttcaac aaaactttct cagcttcctt
    10381 ataattatcc aggtaggcaa agttgccatt acctctttca gccagtagtt gtattttaga
    10441 atccttgtaa ttgcccatac ccacacccag acaggtaaga taaacaccca ggtctttttg
    10501 ttttgtaatt aactcatcaa gttcttcttc tgtttttaaa ccaacattaa agtcaccatc
    10561 tgtagccaat atcaccctat tatttccttc tttaataaaa tgatttcttg cttcccggta
    10621 ggcaattcgg atccctgatt cacccggggt atttccaccg ggctccagtt catcaatcac
    10681 ttttaatatc ttttcttttt ctcctccact ggtgcaattc agcattactc ctgttacacc
    10741 accatacaca acaatggtga cagaatcctt atctctaagg ttattaacca gcaaacgaaa
    10801 tgcagatttg agtaatggta acctgttggg catatccatt gatccggata catcgatcag
    10861 aaaaaccagg ttgctcggcg gcaaagtatc cagatttagt tttttggatg ataggtttat
    10921 ataatacaac tgattatctg tattccaggg gcaggctgtc aaagtagttt ttatatgaaa
    10981 aagatcgcct ccctgcggtt catggtagct gagattaaag taattcagca tttcttctat
    11041 acgtactgca tccgggggca ccagggaatt taaagtaata aaccgccgga tattgctgta
    11101 nnnnnnnnnn nnnnnnnnnn nncatttagc gccaaaccgg tgcttggaaa aagttttgcg
    11161 gcaataaaat tattttccag gagactggca taggtctcat caccggtaaa ccaggttttt
    11221 tgtacttcct tctcaaggtc tttggttaca gacgaaagtt tatccctatg tgcatgggta
    11281 gcactagcag gcagcagttt catttttacc ctgagataat tatctgcatt caccaatagc
    11341 cgttccgttc tatagccttg catggaaaaa ctgaatgtgt caatttgctg attagacagt
    11401 attccaaatg tgccggctga tccgctacga aatacatagc cggttttgtg ctgaagaata
    11461 actgcatttt gtaaaatatt gccagcttca tcagtaacct cgccacgaac ataaaactgc
    11521 tgggcgtata cggacaagct caagaaaagg aatatggcta tatgacaagc ggccttcaaa
    11581 tggatatttt agcccgataa aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnna ctaactgata gatctctttc agattacggc ccaaaccatc
    11701 ataatccagg ccatatccaa ctacaaactt attaggaata tcaaagccaa tataatccag
    11761 tttcagggga aattcagtag cctctgactt gtgtaataga gtggcaattc ttaaggattt
    11821 gggctgctgg tgctccaatt taggtaaaaa attgtggagt gttttgcccg tatcgactat
    11881 gtcttccacg atgacgatct ctttattgaa gatatcatct tccaacccga tagaagtaac
    11941 cacattgccg gaagacttta accctttgta ggaagccagt ttaataaagc ataactctgc
    12001 ttcaattgta aggtgtttga acagatctga tgcaaacatg aaagatccgt tgagtatagc
    12061 tataaacatt ggacgtttac ccgcataatc tgtattgatt gccgcagcaa gttcttgtac
    12121 ccttttttga atagtatcct ctgaaagata tgtttcaaat gttttatcat gcaccttgat
    12181 aagtcccata atctatgtta gttgttagtt tgtcgaattc gtttttatag acggaagcaa
    12241 agaatagttt tctcttacag ccagtttagg catcgaagta gcctttgttt taaggttaag
    12301 cttctccccc gctgccggca tctcattttc tttaagccag ttcatctcct gcaggctttc
    12361 cagcctgata gcatttaact gggcaatatc atgaagtgtc tccccgggtt gaacaatatg
    12421 aaaatcgttt atcccagttt tgcgcttgcg ctgcaaatag atcaattgat cttttgctga
    12481 ttcttgtgcc cttgtaattt cattgaactc aaaaatcttt gccaggtcaa tatcatattg
    12541 ttttgctatt gccaggaaag gagttccttt ttttacaaat actactttgg tttcattgat
    12601 tttaaattcc ccatcagggt acacaggctt ttttgcttgt ggcatttcat caactgctgg
    12661 tttctctgcg ggtaaatcat tttttacaat tatgttatcc gttgcttgtg tattttttcc
    12721 aagggctatc aatgtatact cctgtaaatt gtaatcttca atcagtttaa ttaaaacctg
    12781 ggggtatttg ggattagtag cataccctgc ttttttcagg ccataagccc aaccggtata
    12841 atcggcaggg tcaagttgaa aaagaaaagc gtaatgtttt ccgtttcgaa gaaaatcgga
    12901 atgatccttg taagagtctt ctgattgctt atacttccta aaacattcgc cacgggaatc
    12961 atcgtcatgt tttacagact ctccagtcca gttggatttg catttaattc caaaatgatt
    13021 atttgacatc aacactagtt taccctggcc tgctcctgac tcatgaatac cctgtgccag
    13081 ggtgatggcg gcaggcacac ctgtcctctg catctcggca atagcaaggt ccttgtatgt
    13141 gnnnnnnnnn naatttttga ttatttctga ttgctgcgaa aagacaaccg gtgcaaccag
    13201 cagcattaga gaaagcaaaa tattcctgat cttcagcatg gatattcatt ttatagtttc
    13261 cgcaacaggt ataaaccatc acggatggtt agcactactt tttcaatgtc tgttctctcc
    13321 ctgataaaat cattaaacgc ctggatgcct tttgcgtttt tacccttcac ctcctctttc
    13381 agcacctggc catgaaaaag tatattatct gctaaaataa aaccgttttt ccgcaaccgg
    13441 ggaaaaacga ggttaaaata ttcaatatag gcgggcttat ccgcatctat gaaaacgaga
    13501 tcccagtttt cttccagccc gggtattata tctaacgcat tacctacatg tgaaattatc
    13561 tgtgcagcat aatcagattg gtcaaagtaa gccctcgcca ccctggcatc ttcttctctt
    13621 aactctatag tgtgaagttt tccctgcacc ttcaatccct ttgccaggca tatagcactg
    13681 taaccagtaa aagtgccaat ctccagaatc cggtctgggc tgatcataca gctgatcatt
    13741 tccagcaact ttccctggac acttccgctc agcatatggt gctcagggtg atgctgaaag
    13801 gtatattcac tgatctcttt aagtaaagga ttttctgctg aagagtactt ctcagcgtat
    13861 tgagttacca aaggggctat caggtccaat agaaattttc accaaagata aatctctggc
    13921 acggcaaacc atagacctgt tacattattc cattccctta tcttctatag atgaaatttt
    13981 acccccctcc cagcatatat tatattttct tttcgtcatt cctgatttac actccatcag
    14041 gtaacaatta ccacttgctt ccagcacttt aggcttttca aggttgtaaa gatccatgtc
    14101 tttacggtcg aggccgggtt ttccaaaatc atcgagggcg atttttacgg aagctgaccg
    14161 tccgttgaag ttgttcataa aatcctgcat ttctgtactc acttttacgt cagctttttt
    14221 ttctcctccg ccgcaggcga taagaataaa tacggatacg ggcagtaaaa ctgccaaaag
    14281 aaattttctc ataataactg gtttaaggtt aatggttttt tgcgtactta atattacaaa
    14341 aaaaccggga ctaaacaacc tgcnnnnnnn nnnnnnnnnn nttctgaatg ctttgaagat
    14401 atcatccaca agccaaggaa tgtaaaaatt ccgttgatca atataagttc attaccaatt
    14461 ttataaccat tgaaaatgga ttcggagatg tattgaaggc caagctttac atgaagcttc
    14521 gcctcgtacc aggcaggatt gctcagtata tcaaccgaaa gagccagcaa tggagcaata
    14581 atacaaaccg cccagatcag gttttctttt agcttacgtt tggtaagaat tccaaaggca
    14641 aacagaccca aaaggggccc gtaagtaatg ccggcaaatt tgagaatgaa atcaatgatc
    14701 agcttatcat tgatccattt aaaaatgagc accatgataa aaaataccaa tgcaaaagtg
    14761 aaatgcactt ttaaacgggt attcttctta tgtctctctg tcccctcttt tttattcaac
    14821 cccaggatat caatacaaaa acaagaagtc aacgaggtaa tagccccatc cacactggga
    14881 aataaagccg aaatcagggc gatgataaag atgatgccaa ttgtaccact gaaagcatcg
    14941 ctcaaagcta tagtcggaaa taaatcatcc ggcggtacag caattccctt tgtnnnnnnn
    15001 nnnnnnnnnn nncagtaccc cgcctaatac aaggaacaaa aaatttacaa tcatcaatac
    15061 cgtagtaaag gtcatcatgt tcttttgtga atccttcagg gttttcacac taatattttt
    15121 ttgcatcatc tcctggtcaa gacctgtcat ggcaatggcg atgaacatcc cgccaattat
    15181 atgtttccag atgtaagaag gagccttaac atcccagttg aaaatctggg tataaccttt
    15241 ttctgacatc atggatagtg caccgccaaa atcagtaccc aatgctttta caattacaat
    15301 gatacaggca acgaggccta ccagcatacc ggtagtctgc aacgtatctg tatnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnccttc aaatgtgtac aataagatca atacaagtat
    15421 taccagcgca gtagcttcaa acgggatgcc cagcttatta aaaataaaaa tctgcagtac
    15481 actgatgaca aggtataaac gggcagtagc tcccactgtt ctggacagga taaaaaagaa
    15541 tgcgccggct ttgtgggcat ttataccaaa ccttttttcg aggtaggtat aaatactggt
    15601 taggttcatc cggtaatata acggcaagag cacaaaagca atgaccatat aacccagcag
    15661 gtagccaagc acaatctggt aataataaaa attactggtg cccacactcc cgggaacgct
    15721 aacgaaagtt actcctgaca aggatgtacc aatcataccg aaagccacca gcatccagtt
    15781 actgttgcgg ttgccgataa agaatgattc attattggaa tttcttgaag tataccaggc
    15841 aacgacaagc agtaatacga aatagccaat aacaaacgca aataaaacag ctggggacat
    15901 aatgaaagtt tctgttaatg aataattacc gttgtatgaa atccgctaac gaattttgaa
    15961 gataaagaaa attacattcc ctttgcttca atttacatga actatggcta tcacatcact
    16021 cgccgttttc tgcggctcaa aagatggcat taatcccatc ttttctgaac atgcaagaac
    16081 attgggaaaa ctaatggcag aatacaatgt caggctggtg tatggcggag gcagtgccgg
    16141 catcatgggt attattgcag atagtgtaat gcagcatggt gggaaagtga cagggtttat
    16201 tcccaggttg ctccttgaat gggaggtgca gcaccgtggt attaccgaac tgataatttg
    16261 tgatgatatg catgaaagaa agaagcgaat ctacagtatc agcgatgcag ccattatact
    16321 tccgggtggc tttggcaccc tggatgaact atttgaaatc gttacatgga accagcttac
    16381 catccacgac aaagaaatct acatcctgaa ttcagttggt ttttacaatc acctgataga
    16441 acatattgag gtaatgaagc aggagcaatt cctttacgaa gaagcattaa aaaggatcac
    16501 cgttttagat gatccttccg agctgattac ttatctgaaa taacttttat tcaaatctgc
    16561 ggttacgacc ctggaacagt ccaatgttaa taccagccct gatcaacgga accgccctta
    16621 cacttctgcg gccggtaacc tggtcgtaca ctacatccac atagggcgaa ttctcgtttt
    16681 taatcacatc aaaaagaaca gaaacataat aaaaggtatt ggagcctgcc tgccttccct
    16741 ggcaataacc gccgccaacc aggaatgaat ttgcatccac tgtttcttta tagggctgat
    16801 aatttgaagt agccggcaca taacgaagac taataaaatt atgctc
//
LOCUS       DAORLS010000029        16520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1878_length_16520_cov_2.306347, whole genome shotgun sequence.
ACCESSION   DAORLS010000029 DAORLS010000000
VERSION     DAORLS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1878_length_16520_cov_2.306347"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..860
                     /locus_tag="PK977_03885"
     CDS             48..860
                     /locus_tag="PK977_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRF17278.1"
                     /translation="MRTQHLKWIGPLALVFLSFSKEAAPPQDNFCGIRNIAFKVEEEV
                     SFTVYYAVAGIYVNAGTATFTSKLETINNRPVYHVTGEGKTNSSYDWIYKVRDKYETY
                     IDTATFQPLKFIRNVNEGGYKKYENITFNKTSNTAITGNGVFKVPACVQDVVSSMYYA
                     RNVDFSKLQPDDKIPFSMFLDNEVFNMYIRYVGKEEIKTKYGKFRAIKIKPLLLKGQI
                     FEGGENMTVWVTDDQNHIPVRVESPLVVGKVKIDMMSHTNLRHPLTSLIKKK"
     gene            complement(909..1769)
                     /locus_tag="PK977_03890"
     CDS             complement(909..1769)
                     /locus_tag="PK977_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF17279.1"
                     /translation="MKLYTINTGYFKLDGGAMFGVVPKTIWNKINPADENNLCSWALR
                     CLLIEDGNRLILVDNGNGDKQDARFFSHYYLHGDDTLDKSLAKYGFHRDDITDVFLTH
                     LHFDHCGGTIKREGDKLAPAFKNAVCWSNKDHWDWAVYPNDREKASFLKENILPIQEN
                     GQLQFVDVNDGIDGQLGKTRFTDNIDIRFVSGHTDKMMLPQIRYKDKTIVFMADLLPS
                     AGHMPIPYVMGYDMFPLTTILEKKSFLKEAQEGDYILFFEHDPVNECCTVQQTEKGIR
                     PKDYFKLEDI"
     gene            complement(1766..>2137)
                     /locus_tag="PK977_03895"
     CDS             complement(1766..>2137)
                     /locus_tag="PK977_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF17280.1"
                     /translation="RPFGMCLVVLWILLCVAAYFLPKGGIYEFYGLGAAVGFIMGGIQ
                     SLSRSTYAKLMPETKDTASFFSFYDVTEKMAAVVGIFSFGFITEISGSQRNSVVALAT
                     FFILGLLLLFYANAAKEKQTQ"
     assembly_gap    2138..2185
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2186..3077)
                     /locus_tag="PK977_03900"
     CDS             complement(<2186..3077)
                     /locus_tag="PK977_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF17281.1"
                     /translation="MQTAPKKVVNGWAMYDWASSSYNLVITSTIFPTYFESVAVDNAA
                     ISKTTVTFFGRQFENTALYNYALALAMLIVAVMSPLLSSIADYRGTKKRFLNFYLTMG
                     SIACASMFFFDSQTLELGIAGIIIACVGFWGAQVFYNSFLPEIATIEDRDKVSAKGFA
                     YGYVGSVLLQLICFAFVMGKPFGITEGKGAQISFLLVGLWWWGFGQFALKRLPKPVPA
                     GTGDLQHNIFSKGYSELRKVWNQLTHMPKLKRFLLSFFFYNMGVQTVMLVAALYGKSE
                     LEIPTTNLIIAILIIQLIAIP"
     gene            complement(3162..3926)
                     /locus_tag="PK977_03905"
     CDS             complement(3162..3926)
                     /locus_tag="PK977_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17282.1"
                     /translation="MKKLILSLLAVLMLAACTNYGKKVKEGHIEVYYKEGITQQEAEK
                     TAKALYDADSKAGNDPARKSFQLSREGDTVLLRMVVDQSRAKDMGDENFSPIALMISE
                     NAFNGKPVNMELTDTKFKTIRSIAYQKTTTEDINVAAFGEKITDGNTEVYYKGASTDE
                     ANRLAAFMNEYFKPETTFSFQLIKDENENYIVKMVGNPEKVNTIAVSLFEEVCRGICD
                     KVLSVPAVRFEMTDTEFNAIRTYNYPADTGDPDRNN"
     gene            complement(4094..4921)
                     /locus_tag="PK977_03910"
     CDS             complement(4094..4921)
                     /locus_tag="PK977_03910"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRF17283.1"
                     /translation="MTITDAIIIAIVEGLTEFLPVSSTGHMIITSSLLGIQKDEFTKL
                     FEVAIQFGAILSVIILYWRRFFQFHRWQFYIKLLIGVIPALLLGFLFEDKIDAMLESP
                     TTVAVAMLAGGIVLMFIDSMFKNAKTESDDQITYTQSFITGIWQTIAMIPGVSRSAAS
                     IIGGMSQKFTRNLAAEFSFFLAVPTMFAATAYSVFIKKWTGNGGIERKGYELILESND
                     NLIAFIVGNIVAFVVAMLAIRFFINYLKIYGFRIFGLYRILAGIILLVLIFTGIIKG"
     gene            complement(5001..5246)
                     /locus_tag="PK977_03915"
     CDS             complement(5001..5246)
                     /locus_tag="PK977_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRF17284.1"
                     /translation="MSNEKSTPTMFSKENYMWMLLGIVLIAIGMFLMSGGASNKDPKV
                     FDEAAVYSSTRITIAPILILAGLVIEIIAIFKKPKQA"
     gene            complement(5337..6209)
                     /locus_tag="PK977_03920"
     CDS             complement(5337..6209)
                     /locus_tag="PK977_03920"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRF17285.1"
                     /translation="MSQSGKASIKRGKPSYFMSILGVTLVLFLLGTIGWLVINANKIA
                     RNLKEDVNISAYLRGDLNPKDSSALVNYISQQPYIKSYMYVTKEMAKKIYNSDGNESW
                     DNILTENPLPNSIDFKVKSSYLNPDSLKTIKADLEKQTYVSEVKYPEKFIGKMNENMR
                     NISIVLLVIAIILAAVVIVLIDNTIKLAMFSNRFLIKTMQMVGATRWFIAKPMNIRAV
                     INGAISGIIAIVGVLLLTLIAEKYIPGMKAAHDNLSLFLLFLLMILTGIGITLFSTHR
                     SVVKYLKMKLDDLY"
     gene            6397..9186
                     /gene="leuS"
                     /locus_tag="PK977_03925"
     CDS             6397..9186
                     /gene="leuS"
                     /locus_tag="PK977_03925"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRF17286.1"
                     /translation="MEYNFRDIEKKWQQRWKETNTYKVSNESNKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGYDAFGLPAEQYAIEHGIHPAVSTEA
                     NINNFRSQLDKIGFCYDWDREVNTSKPEYYKWTQWLFLKLYNSYFDKKENKAKPITEL
                     EEQFMVHGSLPLTMNHEPFTADEWKKFDEKKQQEILMNYRLAYSAYGEVNWCEALGTV
                     LANDEVVNGVSERGGHPVEKRKMRQWYLRITAYADRLLEGLNTVDFSDAMKEMQTNWI
                     GKSSGAEVDFAIKGHTQKLRVYTTRPDTIFGVDFMVVAPELELVNEIKSAEQTAAVDE
                     YLAYVKSRSERERMAEKKITGVFTGAYAINPFDGREIPIWTSEYVLAGYGTGAIMAVP
                     CGDERDHKFAKHFNIPITNIIGSHYNGEEANATKEAILENSGFLNGLLMKDAIEVAIK
                     AIEEKGIGVRKVNYKMRDAGFSRQRYWGEPFPIIWKDGVPVALEEKDLPLLLPHMDEV
                     KPGKGGEGPLSNLTNWVNTPEGQRETSTMPGYAGSSWYFLRYMDPANEKEFCSRAASD
                     YWGQVDLYVGGTEHAVGHLLYSRMWTKVLYDLGLIGFDEPFKKLLNQGMIQGSSRFVY
                     RLHGTNTFVSKSLLNNEDEYEGKQLDKIHVDVNMVDGLELDTNAFVIWRNNEHAGAVF
                     ILDDGKYICGVETEKMSKSKFNTVNPDDLVYKYGADTFRMYEMFLGPVEMSKPWDTKG
                     IEGVHRFLKKFWRLFFDEQKGKIWTDEKATSAELKVLHKTIKKIEEDTERFSFNTCVS
                     TFMICVNELTDLKCHKKEILNPLLILITPYAPHIAEELYQQINATQTDEIISMVLDAT
                     FPKWDEKYIKESSKAYPVAINGKTRTELTLSLDITQPEVEALVLQDAIVQKWLDGKVP
                     KKIIYVKNKMINVVM"
     gene            complement(9248..10288)
                     /locus_tag="PK977_03930"
     CDS             complement(9248..10288)
                     /locus_tag="PK977_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17287.1"
                     /translation="MTLIFHCGALFHAGNAQMITGIWHGKINRQKAEVKIIQNGDSLT
                     GTSYYYESANNYRRYSIKGYFDQRTNTTVWWDDHLIEEKSGRFSMNIPGKIPMLSQAD
                     FNCPGGSKMMLDGTVSDRETVAKTLGEVHLDKTGRTIFEDEWDFVIENYTTGANDPDI
                     IDSIAAIAFIPPPTIKKSETTTIPTENKTEVVYTPPVVVEKKQGPVKTTAITTPLTIK
                     EKFVSREKKFIKEIPVAGDSIELKFYDNAEIDGDSISLFINEKLVFEHVRLAATAYIY
                     KIAVTDLKDTTELVMVAENLGKIPPNTSYMVAMVDGVKYDAYLASTEGSSGMIRLVKS
                     KTMKAIETKKEN"
     gene            10464..>11137
                     /locus_tag="PK977_03935"
     CDS             10464..>11137
                     /locus_tag="PK977_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17288.1"
                     /translation="MRKFYFFLLFAFFLTQNFDAKSQCTNTSSFGTATINPSGTIVTI
                     STCSFAGEYSTINGAVSGQTLSFTSSIATDFITIHSGTSNGPVVASGTTPLVFANTFT
                     GTLYAHWNTPGCGAQSSCRTTTVQCTSCSGGGGPCTNTSSFGTATISPVGALVTISTC
                     SFAGEYSTINGAVNGQTLRFTSSVATDFITVRSGTSNGPVVASGTTPLVFANTYTGTL
                     YAHWNTP"
     assembly_gap    11138..11147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11148..>12315
                     /locus_tag="PK977_03940"
     CDS             <11148..>12315
                     /locus_tag="PK977_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17289.1"
                     /translation="GAQSSCRTTTVQCTSCTLPPPANDLCTGALTINCGQTLTGSTAA
                     ATADAVTGCATTLNTAPGVWYTFAGDGSLTTLSLCGSAFDTKIGVFSGTCGTLTCVTS
                     NDDFCGLQSQVTFSANVGTTYYVLVTAFGTNSGNYTLARTCIPACAGVPSPGAISPVT
                     STNCAGTSRTLTLSGYSIANGITFQWKSSPTSGGPYTNIAGATNNTYTFTTASTQYYV
                     CTVTCTNGGGSATTAEVVVNVSNVSHSSITSNPATTCSPGASTISATATGGFGNYTHT
                     LTGPGTIGAPVVSGGNNQNVSFNVTNIPVGANTYTLTSTDANGCSKASTVSVTVNQTP
                     VITITPAAPVICAGNIQQLTANAVTGGSTTLSSGNINLAIPDNNAAGVTTAPISI"
     assembly_gap    12316..12325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12326..>12871
                     /locus_tag="PK977_03945"
     CDS             <12326..>12871
                     /locus_tag="PK977_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17290.1"
                     /translation="GVPITNPADLKISINARHSWSGDLIFKVTSPCGTTYLFDRLGVP
                     PLTFGNSANLGTSSASTPPPAVYTFDLAAATVMPESGPGTGFIPTGSYLPSNTTGVAH
                     NWTGLTFPCTGTGNWTLNVSDNGGGDVGMLVDWSITYSSTVNSPVVFSPLTNVFTDAA
                     ATIPYTGTPVNTVWVKPPTTTT"
     assembly_gap    12872..12902
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13082..14122
                     /locus_tag="PK977_03950"
     CDS             13082..14122
                     /locus_tag="PK977_03950"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF17291.1"
                     /translation="MDGPQVLGSNTATLRLFNVAAANAGNYDCVVSGVCPPPATSNAV
                     ALVVASAPSVTNPANANICAIGATSQTSATFSVTGTGVPTPTIYQWQISTDGGGTWNN
                     LANSVSTAASPYYTGVFTASLTVSNVPTSLDGARFRAVVTNSCAQSTNSGSATLTVRA
                     TPVVVANDLFNQRICISDTLVPLVGSPVGGAWTGIGVSGFNFVPTATAIGTYTLTYTY
                     TNSVNCTARDTTLVKVVDCPERQRLLRDDAILVYPNPNNGLFNIRINSTLYNYLGMKV
                     FDMTGRLVNGKVTKSGIDQQLVSPTWSNLVYGRVIPIDLTYLPSGNYLVEFYYDDGVR
                     TSKKGYVIQILK"
     gene            14268..15296
                     /gene="ruvB"
                     /locus_tag="PK977_03955"
     CDS             14268..15296
                     /gene="ruvB"
                     /locus_tag="PK977_03955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRF17292.1"
                     /translation="MSNSNLNSAKENLAAADIAFENTIRPSVIDDFSGQAQIIENLRI
                     FIKAAKIRGEALDHVLFHGPPGLGKTTLSRIVANEMGVSIKETSGPVIEKPGDLAGLL
                     TNLEPNDVLFIDEIHRLSTVVEEYLYAAMEDYRIDIMIDSGPNARSVQINLNPFTLIG
                     ATTRSGLLTAPLLSRFAIKSRLEYYTAEVLNKIIHRSANILGTNISKDAIEEIGRRSR
                     GTPRIANGLLRRVRDFAQVLNDGQIDLGITQHALKALNVDEHGLDDMDNRILSTIIEK
                     FKGGPVGITTIATAVGEEAGTLEEVYEPFLIQEGFIQRTPRGREVTAKAYEHLGKSKP
                     GTTGTLFS"
     gene            complement(15356..16051)
                     /locus_tag="PK977_03960"
     CDS             complement(15356..16051)
                     /locus_tag="PK977_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17293.1"
                     /translation="MEEKKPKLLLCEDDPNLGSVLKNYLELNDYDVTLERDGRLGLAA
                     FQREKFDLCLLDVMMPNMDGFTLAEEIRDIDPDVPLFFLSAKTMKEDVIQGYKLGADD
                     YITKPFDSEVLLMKIKAILKRNEEQNKESENMEFDLASYHFNPKLRQLIHDGITQTLS
                     PKENDLLKMLAEHLNDLLPREQALKKIWGSDTYFNGRSMDVYIAKLRKYLKDDPKIEI
                     VNIHGNGFRLVVQ"
     gene            16192..>16520
                     /locus_tag="PK977_03965"
     CDS             16192..>16520
                     /locus_tag="PK977_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HRF17294.1"
                     /translation="MHITPDNRLKKLIVIGDRLLIKPTRPDERTASGLYLPPGVQEKE
                     KVQQGYVIKAGPGYAIPMPVEDEPWKGEDDKVKYVPLQAKEGDLAIFLLSGATEVMYE
                     NEKYFIVP"
BASE COUNT         4863 a         3278 c         3434 g         4846 t
ORIGIN      
        1 aagtttatta atagtgcgaa tcaataaaat atttacttcg aataattatg aggactcaac
       61 atcttaaatg gataggcccg ctggcgttgg tttttttgag tttttcaaag gaggcagcac
      121 cgccgcagga taatttttgt ggaatcagga atattgcatt caaagtggaa gaagaagtgt
      181 cttttactgt ctattatgct gtagcaggaa tttatgtaaa tgccgggacc gccactttta
      241 ccagcaagct ggaaacaatt aataaccggc ccgtttatca tgttaccggg gaaggaaaaa
      301 cgaattcttc ttacgactgg atatacaaag tgagagataa atatgaaaca tatatagata
      361 ctgctacatt tcagccactc aaattcattc gtaatgtgaa cgaaggcggt tataaaaaat
      421 acgagaatat cactttcaac aagacttcca acacagcaat tacaggtaat ggtgttttta
      481 aagtgccggc ctgtgtgcag gacgtggtaa gttccatgta ctatgccagg aatgttgatt
      541 tttctaaact gcaacctgat gataaaatcc ctttctccat gttcctggat aacgaagtat
      601 ttaatatgta tatccgctat gtgggcaagg aggagataaa aacaaagtat ggtaagttca
      661 gggctattaa aatcaaaccg ttattattga aggggcaaat atttgaaggt ggtgaaaaca
      721 tgacagtatg ggtaacggat gatcaaaacc atattccggt aagggtagaa agtccgctgg
      781 tggtaggtaa agtgaaaatt gatatgatga gccataccaa ccttcgtcat ccgctcactt
      841 cactgatcaa gaaaaaatag tgctattgat tggataaaga aaagcccctc aaaaaggggc
      901 tttagctatt aaatatcttc cagtttgaaa tagtctttag gtctaatccc tttttccgtt
      961 tgttgaacgg tgcaacattc atttaccgga tcatgctcaa agaaaagaat ataatcaccc
     1021 tcttgtgctt ctttaaggaa tgatttcttt tcaagaatgg tggtcaatgg aaacatatca
     1081 taacccatta cgtaagggat cggcatatga ccagctgatg gaagcagatc ggccataaac
     1141 acaatggttt tgtccttata ccttatctgc ggcagcatca ttttatcagt atgtccgctt
     1201 acaaaacgaa tatcaatatt gtcggtaaag cgggtttttc ccaattgccc gtcaatgcca
     1261 tcatttacgt ctacaaactg tagttgcccg ttttcctgga taggcaaaat attttccttc
     1321 aggaaagatg ctttctcccg gtcgttcgga taaactgccc aatcccagtg atctttattg
     1381 ctccagcaaa cggcattctt aaatgcaggt gccagtttat cgccttctct tttaatagta
     1441 ccaccgcaat gatcaaaatg aaggtgagtt agaaatacgt cggtaatatc atcccggtga
     1501 aaaccatatt tcgccagtga tttatccagt gtgtcatcgc cgtgcaggta gtaatgactg
     1561 aagaacctgg catcttgttt atctccgttg ccgttatcaa ccagtatcag ccggttacca
     1621 tcttcaatca ggaggcaacg taatgcccac gaacaaagat tattttcatc ggcagggttt
     1681 attttattcc atatcgtctt tggcacaaca ccgaacatgg caccgccatc cagtttaaag
     1741 taacctgtat taatcgtata gagtttcatt gtgtttgctt ttctttagct gcattagcat
     1801 aaaataatag taataaacca aggataaaaa atgtagcaag tgctacaaca gagtttctct
     1861 gtgaaccgga tatttctgta ataaacccaa aactgaaaat gcctactaca gctgccatct
     1921 tttctgttac atcataaaaa ctaaaaaagg aggctgtatc ttttgtctcc ggcatcagct
     1981 tggcataggt agagcggctc aatgactgaa taccacccat gataaatccc acagctgctc
     2041 ctaatccata aaattcataa atcccgcctt taggtaaaaa atacgctgct acacataaaa
     2101 gtatccataa tacaacaagg cacatcccaa aaggccgnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnncgggg atggcaatta gttgaattat taaaatagca
     2221 atgatcaggt tggtagtagg aatctccaat tcactttttc catacaaggc agctaccaac
     2281 atcactgttt gtactcccat attatagaag aaaaaggaaa gcaaaaatct ttttagtttc
     2341 ggcatgtgtg tcaactggtt ccatactttt ctcagttctg aatagccctt tgaaaagata
     2401 ttatgctgca aatcacctgt gccggctggg acaggtttag gtaatctttt taatgcgaac
     2461 tgcccaaatc cccaccacca taagcctacc agcagaaaag aaatttgagc cccttttccc
     2521 tctgtaatgc caaaaggttt tcccattaca aatgcaaaac aaataagttg cagcagcaca
     2581 ctgcccacat agccataggc gaaacctttg gcacttacct tatcacggtc ttctatggtt
     2641 gctatctccg gcaggaatga attataaaaa acctgtgcac cccagaagcc cacacaggcg
     2701 ataataatac ctgctatgcc tagctccagt gtttggctgt caaaaaaaaa catggaagca
     2761 caggcaatgc tacccatagt gaggtaaaag ttcagaaatc tttttttggt tcccctgtaa
     2821 tcggcaatag atgaaagcag tggtgacatg acggccacaa tcagcatggc aagggccagc
     2881 gcatagttat aaagtgctgt attctcaaac tggcggccaa aaaatgttac agtggtcttt
     2941 gaaattgctg cattgtctac ggcaacggat tcaaagtaag ttgggaatat ggtggaggtg
     3001 ataacaagat tataggagga actggcccag tcgtacatag cccagccatt tactactttt
     3061 tttggagccg tttgcatacc caatgctttt ggtaaatata gtattaagcc aataaaataa
     3121 aaacccgccg gagtgggacg ggttttttag cttttacata atcagttatt tctgtcaggg
     3181 tcaccggtat cggccgggta attataagta cggatggcat taaattctgt atccgtcatc
     3241 tcaaacctta cagcaggtac tgaaagtacc ttgtcgcaaa tgcctctgca aacctcttca
     3301 aaaagggaaa ccgctatggt atttactttt tccgggttgc caaccatttt cacgatatag
     3361 ttttcatttt catcctttat cagctgaaaa ctaaaagtgg tttcaggttt gaaatactcg
     3421 ttcataaatg cggccagccg gttggcttca tctgtgctgg cacctttata atatacctct
     3481 gtatttccat cggttatttt ttcaccaaag gccgctacat ttatatcttc tgtcgtagtc
     3541 ttttggtagg caatgctcct gatagtttta aacttggtgt cagtaagttc catgttcacc
     3601 ggttttccat taaacgcatt ttcagagatc attaacgcta tcgggctgaa gttttcatct
     3661 cccatatctt ttgcccggct ttggtcaact accattcgca aaagaactgt atcaccttca
     3721 cgggacagtt gaaagctttt cctggcagga tcatttcctg ctttgctatc agcatcataa
     3781 agggctttgg ctgttttttc tgcctcttgt tgggtaatac cttctttata atacacttca
     3841 atgtgccctt cttttacttt tttaccatag ttggtgcagg ctgccagcat tagtacagcc
     3901 aacagcgaaa ggataagttt tttcatgtgt ttatttttta gttcgcccct tcgaggctga
     3961 tttatttgtc acatgttatg ccttattaaa cattcttggt tgttttgttg cgtttgttaa
     4021 ttggcatttc atgttgattt gtttttgcat aaatacacaa aaacaggcaa aattaaatca
     4081 cccatattgg ggattatcct tttatgatcc cggtaaaaat gagcaccagc aatatgattc
     4141 cggccaggat gcggtaaagg ccaaatatcc tgaagccata gatcttcagg tagttgatga
     4201 agaaacgaat agccagcatg gcgactacaa atgcaactat atttcccaca ataaaagcaa
     4261 tgaggttatc attactctcc aggattagct cataaccctt tcgttctatt cctccatttc
     4321 cggtccattt ttttataaaa acagagtagg ctgtggctgc aaacatggtg ggtactgcaa
     4381 gaaaaaatga aaattcagct gcaaggttac gagtaaattt ttgcgacata ccaccaatga
     4441 tgctggctgc gctgcggcta accccgggaa tcatggcaat ggtttgccag ataccggtga
     4501 tgaacgactg ggtataagta atctggtcgt cggattcagt tttagcattt ttaaacatcg
     4561 aatcaatgaa catcaatact ataccacctg ccagcatagc tacggcaacg gtggtgggac
     4621 tttccagcat ggcgtcaatc ttatcttcaa acaggaagcc taacagtaaa gcagggataa
     4681 caccaatcag tagcttgata taaaactgcc agcggtgaaa ctgaaagaac cgtcgccagt
     4741 ataaaataat gacggataga atagcgccaa actgaatggc cacctcaaac aacttggtga
     4801 attcgtcctt ctgtataccc agcaatgaac tggtgatgat catatgaccg gtagatgaaa
     4861 caggaagaaa ctctgttaat ccctccacaa tggcaatgat gatagcgtcg gtgatggtca
     4921 ttcgttctat ttattcgcaa cataataaaa aagcgatcag tcccggtgat cgcttttaat
     4981 aaaatatcaa aaaattttga tcaggcttgt ttcggctttt tgaagatggc aatgatctca
     5041 atcaccagcc cggccagtat caggatcggg gcaatagtga tgcgggtaga actgtacact
     5101 gctgcctcgt caaaaacttt aggatcttta ttgctggctc cgccagacat caggaacata
     5161 ccaatagcaa ttagtacaat acccagcagc atccacatat aattttcttt actaaacatg
     5221 gtgggtgtac ttttttcatt actcattttt tcaatttttg gagttgcaat ataggaaata
     5281 agtcaatggc gaatgggttg taatttcttt attgattact agccagtcac taatggctag
     5341 tagagatcgt ccagcttcat tttcaggtat ttcaccacac tgcggtgggt gctgaacagt
     5401 gtaataccaa ttccggtaag gatcatcagt aaaaataaaa ggaacaggct caggttatca
     5461 tgagcggctt tcataccagg aatatatttt tcagctataa gcgtaagcag taaaaccccc
     5521 acaatggcaa tgataccact gatggcgccg ttaatcaccg cccggatatt cataggcttc
     5581 gctatgaacc agcgggtagc ccctaccatt tgcatcgttt ttatcaggaa gcggttgctg
     5641 aacatggcca gtttgattgt attatcaata agcacaataa caacggccgc cagtataatg
     5701 gcaataacca gtaaaacgat actgatgttc cgcatattct cattcatctt gccgatgaat
     5761 ttttccgggt attttacctc gcttacatag gtctgttttt ccaggtctgc ttttatcgtt
     5821 ttaagcgaat ccgggttcag gtaagaactt tttaccttga agtcaatact gttgggaagc
     5881 ggattttctg tcaggatatt gtcccagctt tcattaccgt cactgttgta aattttttta
     5941 gccatttctt tggtcacata catataactt ttgatgtaag gctgctggct gatatagttt
     6001 acgagggcag agctgtcctt tggattaaga tctcctctta aataagcact gatattgaca
     6061 tcctctttca ggttcctggc aattttgttg gcattgataa ccagccagcc aatggtgcca
     6121 agaagaaata ataccagtgt gacccccaaa atggacatga agtaagaagg ttttccacgt
     6181 ttaatcgaag cttttccgga ttgggacatg aacaattgtt tttcagatga ataggttggg
     6241 caaaaataac agataaagca gtacaatcag tatttttgct gcccaataaa acgattgccc
     6301 ccggttcata ttgccatttg tggttgaaga taattgttaa agatttacgg aaaacattga
     6361 tatcgtttca aaatttataa aggcaggctg ataaatatgg aatacaattt cagggatatt
     6421 gaaaagaaat ggcagcaacg ctggaaggaa accaatacat acaaggtttc gaatgagagc
     6481 aataagccga agttttatgt gctggatatg ttcccatatc ccagtggtgc cggcttgcat
     6541 gtaggtcatc cgctgggtta tattgcatca gatatctata gccggtacaa aagattgaaa
     6601 gggttcaatg tattgcaccc gatgggttat gatgcgtttg gtttgcctgc cgaacaatac
     6661 gctattgaac atggtattca tcccgcagtt tccacagaag cgaatatcaa taacttccgc
     6721 agccagttag ataaaatcgg tttttgttat gactgggaca gggaagtcaa tacatcaaag
     6781 cctgagtatt ataagtggac acaatggcta ttcctgaaat tgtacaatag ctatttcgat
     6841 aaaaaagaaa ataaagcaaa acccatcaca gaactggaag aacagttcat ggttcacggt
     6901 tcattgccat taacgatgaa ccatgaaccc tttacggcag atgaatggaa aaaatttgat
     6961 gaaaaaaaac agcaggaaat cctgatgaac tatcgccttg cctacagcgc ttatggcgaa
     7021 gtgaactggt gcgaagcact gggaacggtt ttggccaacg acgaggtagt gaacggggta
     7081 agtgaaaggg gaggccaccc ggtagagaaa agaaaaatgc gtcaatggta cctgcgtatt
     7141 acagcttatg cggatcgcct gctggaagga ttgaatacgg ttgacttcag cgatgcaatg
     7201 aaggaaatgc agactaactg gattggcaag tcaagcgggg cagaagtaga ttttgccatt
     7261 aaagggcata cacaaaagct gcgggtttac actacacggc cggataccat ctttggcgtt
     7321 gactttatgg tggtggcacc ggaacttgaa ctggtcaacg aaataaaatc tgctgaacaa
     7381 actgccgctg tggatgaata cctggcttat gtaaaaagcc gcagtgaaag agaaaggatg
     7441 gccgagaaaa aaataaccgg tgtgtttaca ggggcctatg ccatcaatcc tttcgacgga
     7501 agggagattc ccatctggac atcggaatat gtactggcag gttatggtac cggggccatt
     7561 atggccgtgc cctgcgggga tgaacgggat cataagtttg cgaaacattt caatattccc
     7621 attaccaata tcatcggcag tcattataat ggggaagaag ccaatgccac caaagaagcc
     7681 atattggaaa acagcggctt tttaaacggt ttgctgatga aagatgccat tgaagtggca
     7741 ataaaagcga ttgaagaaaa aggcatcggt gtaagaaaag taaattataa aatgcgggat
     7801 gccggtttca gccgccagcg ttactggggc gaacctttcc cgatcatctg gaaagacggc
     7861 gtacctgttg cgctggagga aaaagatttg ccgttgctct tgccgcatat ggatgaagta
     7921 aaacccggca aaggtggtga agggcctctt tctaatttga caaattgggt aaatactccc
     7981 gaagggcagc gggaaaccag tacgatgccg ggctatgccg gcagcagttg gtattttctc
     8041 cgctacatgg acccggcaaa tgaaaaagaa ttttgcagcc gggcggcaag cgattattgg
     8101 gggcaggtag atttatatgt aggaggcacc gaacatgctg tggggcattt gctttacagt
     8161 aggatgtgga ccaaagtgct gtatgacctt ggcttgattg gatttgatga accctttaaa
     8221 aaactgctca atcagggaat gatacaaggg agcagcaggt ttgtatatcg cttgcatggc
     8281 acaaatactt ttgtttctaa atctttattg aacaatgaag atgaatatga aggaaagcaa
     8341 ttggataaaa tacatgtgga tgtaaacatg gtggacggcc tggaattgga tacaaatgcc
     8401 tttgtaatct ggagaaataa tgaacatgca ggtgctgtct ttattctgga tgacggcaaa
     8461 tacatctgcg gtgtagaaac cgaaaaaatg tccaagtcga agttcaatac cgtaaatccc
     8521 gatgatttgg tttacaaata cggggccgat acttttcgta tgtatgaaat gtttctgggt
     8581 ccggtggaga tgagcaaacc ctgggatacc aaaggcattg aaggagtaca ccggtttctt
     8641 aagaaattct ggcgtttatt ttttgatgag caaaagggaa aaatttggac ggatgaaaag
     8701 gcaacatcag ctgaattgaa agtactgcac aagacgataa agaaaattga agaagataca
     8761 gaacggttta gtttcaatac ctgtgtcagt acatttatga tttgtgtaaa tgagcttact
     8821 gatttgaaat gtcataaaaa agaaattctc aatccattac ttattttaat tacaccttat
     8881 gcgccacata ttgctgaaga attgtatcag caaataaatg cgacacaaac cgatgaaatt
     8941 atcagcatgg ttttggatgc aacatttcca aaatgggatg aaaaatatat taaagaaagt
     9001 tcaaaagctt atcccgttgc tatcaacggt aaaacaagaa cggagctcac cttatcatta
     9061 gacataacac aaccggaagt ggaagcatta gtacttcagg atgctattgt acaaaaatgg
     9121 ctggatggta aagtgcccaa aaaaatcatt tatgtaaaaa acaagatgat aaatgtggta
     9181 atgtaaagca aaaccgtttt tattaataag caaatgaata attactaatt gtagctgtat
     9241 aaaataatta attttctttt tttgtttcta ttgctttcat ggtttttgac tttaccaacc
     9301 tgatcatgcc actgctgcct tcggtgctgg ccagataggc gtcatatttt acgccatcta
     9361 ccatggctac catataagaa gtgttgggcg gaatttttcc cagattttca gccaccatga
     9421 ccagctcggt agtgtctttt aaatcggtta ctgctatttt gtagatataa gcggtagcag
     9481 ccagtcttac atgttcaaaa actaattttt catttatgaa aagcgaaatg gagtcgccgt
     9541 caatttcggc attgtcgtaa aattttaatt caatagaatc tcctgccacg ggtatttctt
     9601 tgataaattt cttttccctg cttacaaatt tttctttgat agttaatggg gtagttattg
     9661 cagttgtttt tactggcccc tgcttttttt caacaacaac gggtggagta tatactactt
     9721 ctgtcttatt ctcagtaggt atagttgtag tttccgactt ctttattgtt ggaggaggta
     9781 taaaggcaat agctgcaata ctgtcaataa tatccggatc attggcacct gtagtatagt
     9841 tttcaattac aaaatcccat tcgtcttcaa aaattgttct accggtttta tccagatgca
     9901 cttcgcccaa tgttttggct actgtttccc tgtctgaaac tgtgccatcc agcatcattt
     9961 ttgagccacc gggacagtta aagtcggcct gagaaagcat tgggattttg ccgggaatat
    10021 tcatactaaa tcttcctgat ttttcttcaa tcaaatggtc gtcccaccat acggtggtat
    10081 tggttcgctg gtcaaaatag cccttaatgc tgtatcggcg gtaattattg gccgattcgt
    10141 agtaataaga tgtaccggta aggctgtcgc cattttgaat aatctttact tcggcttttt
    10201 gccggtttat ttttccatgc cagatgccgg ttatcatttg ggcattacct gcatgaaata
    10261 aagccccaca atggaaaatc aacgtcaaaa atttcaatct tattggatat cgtagtattt
    10321 tgatttgtag cattggccat ataacggatt ttgccaatta atattttggc ctaaaaatgt
    10381 tggtgcacaa aaaaaaatct taacttccac ccgtaaagcc ctatcaaaag ggtttgtact
    10441 attcatttaa ccaaactttg tttatgagaa agttttactt ttttctacta tttgcttttt
    10501 ttctaacgca aaattttgat gcgaagagtc agtgtacaaa tacttcttcg ttcggcactg
    10561 caactattaa tccgtcagga actatagtta caatttcaac atgctcattt gccggagagt
    10621 attcaacaat taatggagcc gtaagtggcc aaacattaag ttttacgagt agtatagcta
    10681 cagattttat taccattcac tccggtacct ccaatggacc ggttgtagca tccggcacta
    10741 ctccgctggt gtttgcaaat acatttacgg gcactttata tgcacactgg aatacaccgg
    10801 gctgcggagc tcaatcttca tgccgtacta caactgtaca gtgtactagt tgttctggag
    10861 gaggtggacc atgtaccaat acttcttcat tcggaaccgc aacgattagt ccagtagggg
    10921 cacttgtaac tatttctact tgttcatttg ccggagagta ttcaaccatt aacggagccg
    10981 taaacggcca aacattaaga tttacgagta gtgtagcaac agattttata actgttcgct
    11041 caggcacttc taatggaccg gttgtagcat ctggtactac cccgctggtg tttgcaaata
    11101 catatacggg cactttatat gcacactgga atacaccnnn nnnnnnngga gctcaatcct
    11161 catgccgtac tacaactgta cagtgtacca gttgtacgct gcctccacct gcaaatgatt
    11221 tatgtacggg tgcattaact attaattgcg gacaaacact taccggaagc accgcagctg
    11281 caacagcaga tgcagttaca ggatgtgcaa ctacgctcaa cactgcgccc ggtgtttggt
    11341 acacctttgc aggtgatggg tctttgacaa ctttaagttt atgcggctcc gcttttgata
    11401 ctaaaattgg tgtgtttagc ggtacttgtg gtaccctcac ttgtgtcacc agcaatgatg
    11461 atttttgtgg tcttcaatct caggttactt tttctgctaa tgtaggtacc acttattatg
    11521 ttttagttac tgcatttggc actaatagtg gaaactatac tttagctaga acctgtattc
    11581 ctgcttgtgc cggtgtgcct tcacccggtg ctatttctcc ggttacatca acgaactgtg
    11641 caggtacttc cagaacttta acactgagtg gttattcaat tgcgaatggc attactttcc
    11701 aatggaaatc atcaccaact tccggtggtc cttatactaa tattgcaggt gcaaccaata
    11761 atacttatac atttacaaca gcttctactc aatattatgt atgtacagta acctgtacca
    11821 atggtggtgg cagtgctact acggctgaag tagtggtaaa cgtaagtaat gtatctcaca
    11881 gcagtattac ttccaatccg gctacaactt gctcaccggg agcatctact atttcagcta
    11941 ctgcaacagg tggatttgga aactatacac atacattaac cgggcccggt actattggtg
    12001 caccggttgt tagcggaggc aacaatcaaa atgtaagttt caatgttact aatattccgg
    12061 tgggtgcaaa tacatatact ttaacttcaa ctgatgcgaa tggttgttca aaagcatcaa
    12121 cagttagtgt aactgtaaat caaactccgg ttattaccat tactcctgct gctccggtta
    12181 tttgcgccgg aaatattcaa caattaactg cgaatgcagt aacaggtgga agcactactt
    12241 taagttcagg taacatcaat cttgctattc ctgataataa tgctgcgggt gttactacgg
    12301 cacctatctc cattcnnnnn nnnnngggtg ttcccattac caaccctgct gatttaaaaa
    12361 tcagtattaa tgcaagacac tcctggtcgg gtgatcttat ctttaaagta acaagccctt
    12421 gtggtactac ctacctgttt gacagattgg gtgttcctcc attgacattt ggaaactcag
    12481 caaatctggg tacatcaagt gcaagtacac caccacctgc tgtttataca tttgatttgg
    12541 ctgctgccac ggtaatgcca gaatcgggcc ctggtacagg ctttattcct accggtagtt
    12601 atcttccttc caatacaacc ggtgttgcac ataactggac cgggcttact ttcccttgta
    12661 caggtacagg aaactggaca ttaaatgtgt cagataatgg aggtggtgat gtgggtatgc
    12721 ttgttgactg gtcaattaca tatagctcaa ccgttaactc gccagttgta ttcagtccgc
    12781 taacaaatgt atttacagat gcagccgcaa caattccata tacgggcaca ccggtaaata
    12841 ctgtttgggt gaaaccacca acaactacta cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nncagtaagc ggttgtacag catctgcgaa tgtaaccgtt actgtaaatc aactgcctgc
    12961 aataacagtg cagcctacac ctgccacaca aacagtttgc ccaggtgcaa atattattta
    13021 ttcagtatct gcaacaggta ccggtattac ttagcagtgg agaagaaatg gcgtaaactt
    13081 agtggatggt ccgcaggtat taggctcaaa tacagcaaca cttagattgt ttaatgtagc
    13141 tgctgcaaat gcgggcaact atgattgtgt agtaagtggt gtttgtcctc cgccagctac
    13201 atcaaatgct gttgctttgg ttgttgcttc tgcaccttca gttaccaatc ctgcaaatgc
    13261 taatatttgt gcaataggtg ctaccagtca aacttcagcg acatttagtg taaccggtac
    13321 cggcgttcct acgcctacta tttatcaatg gcaaataagc actgatggtg gtggtacctg
    13381 gaacaatctg gctaactcgg tttctacagc tgctagtcca tattataccg gagtgtttac
    13441 tgcttcgctt accgtttcaa atgttcccac ttcattagat ggtgcccgtt tcagagcggt
    13501 ggttaccaat agctgtgcac aatcaaccaa ctccggatct gctactttaa cagtacgtgc
    13561 tacgccggtt gttgttgcca atgatttatt taatcaaaga atctgtatca gcgatacatt
    13621 ggttccatta gtaggatcac ctgtaggtgg cgcctggaca ggtattggcg tttccggttt
    13681 caattttgtg ccaacagcaa cagcaatagg aacatatacg cttacttata cttatacaaa
    13741 tagtgtaaac tgtactgccc gtgatactac tttggtgaaa gtggttgatt gtcctgaaag
    13801 acaaagatta ctgagagatg atgccatact ggtgtatcca aatccaaata acggtctgtt
    13861 caacattcgt atcaatagta ccttgtacaa ctatcttggt atgaaagtat ttgacatgac
    13921 aggccgatta gtaaatggta aggttacaaa gagtggtatt gatcagcaat tagtatcgcc
    13981 tacctggagc aatctggttt atggcagagt aattccgatt gatctgacct atttgccttc
    14041 cggtaattat ctggttgagt tctactatga tgatggtgta agaacttcca agaaaggtta
    14101 tgtgattcag atattgaaat agtcaagcac atcatatttc ttactacatc ccgccagccg
    14161 gcgggatgtt tttttgtaat gctatttaga ttttgggaat gataacttaa gcaaaaattg
    14221 gatttgataa ttaatattta gttttacaat ctagagaaga taaattcatg tccaactcca
    14281 atttaaatag tgctaaagaa aatctggctg ctgccgatat tgcttttgaa aatacgattc
    14341 gcccatcggt cattgatgat ttttcggggc aggcgcaaat aattgaaaac ttacgcatat
    14401 ttattaaagc tgcaaaaatc aggggcgagg ctttagatca tgtattgttt catggcccac
    14461 cgggactggg aaaaacaacg ctttcccgaa ttgtagcaaa tgaaatggga gtgagcataa
    14521 aagaaacatc aggaccggta atagaaaagc cgggagattt ggcagggctg cttacaaatc
    14581 tggaaccaaa tgatgtactc tttattgatg aaatacaccg cctcagcact gtagtggaag
    14641 aatatttata tgcagcgatg gaagattatc gtattgacat aatgatagac agtggaccca
    14701 atgcccgtag tgtgcaaatt aatctgaacc cttttacgct tattggcgct actaccagaa
    14761 gtggattact gacggctccg ctattgtctc gttttgccat aaaatcaaga ttggaatatt
    14821 acacagccga agtgttgaat aaaataattc atcgttctgc aaatattcta ggaacaaata
    14881 tttcaaaaga cgccattgaa gaaataggaa gaagaagccg gggcacacca cgtattgcca
    14941 atggcttatt gagaagggta agagattttg cccaggtatt aaatgatgga cagattgatt
    15001 tgggtattac ccaacatgca cttaaagctt tgaatgtgga tgaacatgga ttagatgata
    15061 tggataatcg catcctttca accattattg aaaaatttaa aggtggtccc gtgggcatta
    15121 ctaccattgc cacagccgtg ggcgaagaag ccggcacctt agaagaagtg tatgaacctt
    15181 ttttaataca ggaaggtttt atacaacgta ctccacgtgg cagggaggtt acagcaaaag
    15241 cttatgaaca tctcggaaaa agcaaaccgg gcaccacagg tactttgttc tcttaaaaca
    15301 agaaagggat tacaacaatc ggtaatccct ttttattttc ttttgagtta ttaaattatt
    15361 gtactacgag cctgaagccg tttccatgga tatttacaat ttcaattttt ggatcatctt
    15421 tcaggtattt gcgcaatttg gcaatataaa cgtccatact gcggccatta aaataagtat
    15481 cgctgcccca aatctttttt aacgcctgct ctctgggcaa caaatcattt aaatgctcag
    15541 ccagcatctt caacaagtca ttttctttgg gcgaaagcgt ttgtgtaatg ccatcatgaa
    15601 tgagttgacg cagtttggga ttgaaatggt agctggccag atcaaactcc atattttcgc
    15661 tttctttatt ttgctcttca tttcgtttca atattgcttt tattttcatc agcaatactt
    15721 cactgtcaaa cggtttggta atataatcat cggctcccag cttatatcct tgtattacat
    15781 cttccttcat cgttttggcg cttaggaaaa ataaaggcac atcaggatcg atatctctga
    15841 tttcttcagc cagtgtaaag ccatccatat tcggcatcat tacatcgagc aggcagaggt
    15901 caaatttttc tctttgaaaa gcggccaacc ccagtcggcc atcccgttcc aacgttacat
    15961 cataatcatt tagctccaga taatttttta aaacgctgcc gagattggga tcatcttcgc
    16021 agagtaaaag cttaggtttc ttttcttcca ttttaggtgg ttttatttat tgttacaaat
    16081 aaagataaag catttatagg cttcaagaat actgattcaa ttttttgtta aaaggatgct
    16141 atttggcctt attttgcttt ttacatttgt attataaaaa aaaactatgc catgcatatc
    16201 actcccgata atcggctgaa aaaattaatc gtaatcggcg atcggttgct gataaagccc
    16261 acaagaccgg atgaaagaac agcctcgggt ttataccttc cgccgggcgt acaggaaaaa
    16321 gaaaaagtac aacagggtta tgtaataaaa gcgggtccgg ggtatgctat acccatgccg
    16381 gttgaagatg agccctggaa gggtgaagat gataaagtaa aatatgtgcc gctacaggca
    16441 aaggaaggag accttgccat ttttctattg agcggcgcca ctgaagtgat gtatgaaaac
    16501 gaaaaatatt ttatagtacc
//
LOCUS       DAORLS010000030        16464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1886_length_16464_cov_2.020172, whole genome shotgun sequence.
ACCESSION   DAORLS010000030 DAORLS010000000
VERSION     DAORLS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16464
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1886_length_16464_cov_2.020172"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>291
                     /locus_tag="PK977_03970"
     CDS             15..>291
                     /locus_tag="PK977_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRF17295.1"
                     /translation="MTIVCGDSHTSTHGAFGSIAFGIGTSEVEMVLATQCLLQSKPKL
                     MRINVDGELSKGVVSKDIVLYILSKITASGATGYAVEFAGSAIRSLSM"
     assembly_gap    292..301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            328..840
                     /locus_tag="PK977_03975"
                     /pseudo
     CDS             328..840
                     /locus_tag="PK977_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
     gene            921..1052
                     /locus_tag="PK977_03980"
     CDS             921..1052
                     /locus_tag="PK977_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRF17296.1"
                     /translation="MSTSNRNFEGRQGAGARTLLASPLTAAAAAITGSITDVRELLN"
     gene            1055..1639
                     /gene="leuD"
                     /locus_tag="PK977_03985"
     CDS             1055..1639
                     /gene="leuD"
                     /locus_tag="PK977_03985"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRF17297.1"
                     /translation="MKALTTIQSRFVPVNIENIDTDQIIPARFLKATTKEGFGKNLFR
                     DWRYYGDDESKTRADFPLNRSQFDGEILVAGKNFGCGSSREHAAWALADYGFRVVVSS
                     FFADIFRNNALNNGVLPVQVSEAFLQRIFLQDNNTELTVDLASQSITINSTGEQETFE
                     INSYKKTCLLNGYDDIDYLMSIKEDIKQFETREA"
     gene            1646..>1740
                     /locus_tag="PK977_03990"
     CDS             1646..>1740
                     /locus_tag="PK977_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRF17298.1"
                     /translation="MKKRIAVIEGDGIGPEVTRQAVKVLNAVAAA"
     assembly_gap    1741..1846
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1847..>2157
                     /locus_tag="PK977_03995"
     CDS             <1847..>2157
                     /locus_tag="PK977_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRF17299.1"
                     /translation="NSDAILFGAIGHPKYDNDPAAKVRPEQGLLKLRKSLQLYANIRP
                     VTTYSSLFHLSPLKSKNIEGVDFIIYRELTGGIYFGKKETAADGSQASDDCTYSREEI
                     ER"
     assembly_gap    2158..2168
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2169..2774
                     /locus_tag="PK977_04000"
     CDS             <2169..2774
                     /locus_tag="PK977_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRF17300.1"
                     /translation="HLAFQHARKRKNKLTLVDKANVLETSRLWRKVVQELALQYSDVA
                     VDYMFVDNAAMQIILNPKQFDVILTENMFGDIISDEASVISGSLGLLPSASVGKGAAL
                     FEPIHGSYPQAAGKDIANPTGSILSAAMLLDHLELKEEAELVRDAVNWTLQNSFVTKD
                     IDPVNFYFTSTIGELICDYISGKISGTVNTENIELRKSTII"
     gene            3065..3958
                     /gene="ilvE"
                     /locus_tag="PK977_04005"
     CDS             3065..3958
                     /gene="ilvE"
                     /locus_tag="PK977_04005"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HRF17301.1"
                     /translation="MYYNENTIIYFTGQFVKAAEAKTDLYSQSLHYGYAVFEGIRSYP
                     VNGGSKMFKGKEHYDRLRRSAEVMKIPFSYSTDQLVELTYETLRRNGFTDAYIRPIVI
                     CSPNMALSKGKESYLAIECWEWTNGYMSNKMKIMTSSYRRPNADAFHVDAKVSGHYVN
                     SILACQEAKDNGFDEALVLDSNGYVAESSGANIFYENNGVLYTAPKGNILPGITRATV
                     LEICEELNIPVQEKLFTPEKMRGADAGFFCGTAAEIVGLSSLDNVPFAKEWEDTLSYV
                     IQQAYKCKVLEKPFQLEQKVA"
     gene            3958..>4729
                     /locus_tag="PK977_04010"
     CDS             3958..>4729
                     /locus_tag="PK977_04010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRF17302.1"
                     /translation="MMNELNKYSKTLTQDPTQPAPQAMYYAIGFKQEDFDKAQVGIVS
                     MGWDGNPCNMHLNDLATSVRNSVNATEGLLGLRFYTIGVSDGISMGTDGMRYSLVSRD
                     VIADSIETNAGAQYYDAIIAIPGCDKNMPGAIMAMGRLNRPAIMLYGGTIAPGHYKGE
                     DLNIVSAFEALGQKIAGNLSEEDFKGIVQHSCPGAGACGGMYTANTMASAIEAMGMSL
                     PNSSSNPALSAEKQRECAAAGEAIKVLLEKNITPKDIMT"
     assembly_gap    4730..4831
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4832..>5219
                     /locus_tag="PK977_04015"
     CDS             <4832..>5219
                     /locus_tag="PK977_04015"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRF17303.1"
                     /translation="GLSLMQDDFQEISNRIPVLADFKPSGKYLMEDLHKHGGVPAVMK
                     YLLKNGLLHGDCLTVTGKTVAENLAGVPDLDFEAQKIIYPLEQPLKKTGHLQILYGNL
                     AKEGSVAKISGKEGEEFEGTARVFDG"
     assembly_gap    5220..5319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5320..5676
                     /locus_tag="PK977_04020"
     CDS             <5320..5676
                     /locus_tag="PK977_04020"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRF17304.1"
                     /translation="APGMPEMLKPTSAIIGAGLGKSVALITDGRFSGGTHGFVVGHIT
                     PEAYEGGLIALVKDDDIIRIDAKKNKITLKVNKEEIKKRRKKWKQPALKASSGILYKY
                     ARMVKPASEGCVTDEE"
     gene            5709..>6149
                     /locus_tag="PK977_04025"
     CDS             5709..>6149
                     /locus_tag="PK977_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRF17305.1"
                     /translation="MDTLELKKAKPQPKALKNLSGSVAVLEALLAEEVTTIFGYPGGA
                     IMPIYDALYDYKDKLKHILVRHEQGAIHAAQGFARVSGKTGVVFATSGPGATNLVTGL
                     ADAMIDSTPVVCITGQVFAHLLGTDAFQETDVINITTPVTKWNYQ"
     assembly_gap    6150..6246
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6247..7471
                     /locus_tag="PK977_04030"
     CDS             <6247..7471
                     /locus_tag="PK977_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF17306.1"
                     /translation="DITKNAQLQLFEYQGYTKCEHIRSYRPKPIVRKEYVEAAAKLIN
                     KAERPFVVFGQGVILGKAEKEFKQFIEKAGIPAAWTIMGMSAIPTDHPLGAGMLGMHG
                     NYGPNVLTNECDVLIAIGMRFDDRVTGRLDKYAKQAKVIHLDIDPAEIDKNVKADVPV
                     WGDCKETLPMLTALVEQKVYPQWLQKFNDYKQKEEEKVIKDELNPTGEVMTMGEVIKT
                     LNELTNGDAIIVTDVGQHQMVACRYAKYNHSKSNITSGGLGTMGFALPAAIGAAYGDT
                     SRTTIALIGDGGFQMTLQELGTIMQFKPNVKIIILNNQFLGMVRQWQQLFHEKRYSFV
                     DITSPDFVQVAKGFGIPGKSISKRGELKDSLKQMLEQDGSFLLEIMVGKENNVFPMVP
                     QGRGVAECVLSKEEI"
     gene            7522..8112
                     /gene="ilvN"
                     /locus_tag="PK977_04035"
     CDS             7522..8112
                     /gene="ilvN"
                     /locus_tag="PK977_04035"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRF17307.1"
                     /translation="MTKKEFTITVYTENQVGLLNRIAIIFSRRKINVESLNTSPTEVD
                     SVHRFTIVINETEEVVRKLCRQIEKQVEVLKAYYNTEHEIVWQELALYKVPTDVIAAE
                     VKVERLLRENGAHTVVIRKDYTVFAVAGHREETEALIKVLEPFGLIEFVRTGRVAIIK
                     GSSGFHERLKEFEQTEPGEEVLENEYLDKNEEVFTM"
     gene            8154..>8407
                     /locus_tag="PK977_04040"
     CDS             8154..>8407
                     /locus_tag="PK977_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRF17308.1"
                     /translation="MAKLNFGGVEENVVTREEFPLSKAQEVLKNETVAVIGYGVQGPG
                     QALNQRDNGINVIVGQRKNSKTWDKAIADGFVPGETLFEI"
     assembly_gap    8408..8463
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8464..>9209
                     /locus_tag="PK977_04045"
     CDS             <8464..>9209
                     /locus_tag="PK977_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRF17309.1"
                     /translation="IALWPTVQKYLTPGKALYFSHGFGVTFNEQTGIVPPKEVDVFLV
                     APKGSGTSLRRMFLQGRGLNSSYAIYQDATGRAFERVIALGIAVGSGYLFETDFKKEV
                     YSELTGERGTLMGAIQGFAAQYQVLRERGHSPSEAFNETVEELTQSLMPLVAENGMDW
                     MYANCSTTAQRGALDWWKKFRDATAPVFNELYDSVAAGKESQRSIESNSKTDYREKLE
                     EELKELRESELWQAGKTVRSLSPENQTKNS"
     assembly_gap    9210..9219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9321..10574
                     /gene="ilvA"
                     /locus_tag="PK977_04050"
     CDS             9321..10574
                     /gene="ilvA"
                     /locus_tag="PK977_04050"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRF17310.1"
                     /translation="MIASTHNVQLDFAAASLRLKKVVNRTPLSFNHNLSRKYQCNVYL
                     KREDLQVVRSYKLRGAYNMLSTLSSEQLEKGVVCASAGNHAQGFAYSCKKLNTRGVIY
                     MPVITPRQKISQTKMFGEGNIEIKLSGDTFDDCAAAAKKFTEENSMTFIPPFDDYRII
                     EGQGTVGTEILEDLSDIDFLFIPVGGGGLSAGVGTYFKTYSPNTKIIGIEPEGAPSMS
                     EALKNGYPVTLETINRFVDGAAVKRAGEITFGICMEVLDDMHLVAEGKVCTTILKLYN
                     EDAIVVEPAGALSIAALDDYAGEIKGKNVVCIVSGSNNDIDRMQEIKERSLQYEGLKH
                     YFLISFAQRPGALKEFVNNVLGPEDDITRFEYMQKTNKENGPALVGVELQSRSDYDLL
                     VKKMKESNIDFAEINKNDQLFGYLI"
     gene            10713..>11397
                     /locus_tag="PK977_04055"
     CDS             10713..>11397
                     /locus_tag="PK977_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRF17311.1"
                     /translation="MAGNQGLYDPSFEHDACGIGFVANIKGHKSHQHISDALTVLENM
                     EHRGACGCEANTGDGAGIMIQTPHEFFFDECLKLGVHLPSFGKYAAGMVFFPKEIRLR
                     EECRDILNRSAEKLGLEILTYRKVPVNTESIGPTALSVEPEMEQVFIACPDHISNPDD
                     FERKLFVLRNYASHLINSTVRKDEIGFYIASLSYKTIVYKGQLTSHQVRQYFPDLSNK
                     RVVSSFGLVH"
     assembly_gap    11398..11428
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11429..>15160
                     /gene="gltB"
                     /locus_tag="PK977_04060"
     CDS             <11429..>15160
                     /gene="gltB"
                     /locus_tag="PK977_04060"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRF17312.1"
                     /translation="FPSWKLAQPFRFIAHNGEINTLQGNLNWLKTSEKGFTTPYFSKE
                     EMEMLLPIVTQGQSDSACLDNMIELLTLTGRSLPHVMMMLIPEAWDGNEEMDPVKKAF
                     YEFHSSFMEPWDGPASISFTDGKIIGATLDRNGLRPSRYCVTDDDRVIMASETGTLPV
                     DPASIIEKGRLQPGKMFVVDMEKGKIISDEDLKKEICSQKPYSDWLNKYKIRLEELPE
                     PRIMFTHLEHDQVFKYQKAFGYSTEDLDTIIAPMAVDGKEPIGSMGTDVPLAILSDQP
                     QHLSSYFKQLFAQVTNPPIDPIRERLVMSLSTFAGNNGNLLVEDPLTCHSVALKHPVL
                     TNYELEKIRSIDTGLFQSKTIQCYFRADGKPGSLKKGLERICEYAVHAVEDGFKVLIL
                     QDRAIDSNHAPIPSLLSTSAIHHHLIRKGMRGQVGIIVEAGDVWEVHHFACLIGFGAT
                     AVNPYLALSSIRDLKETGKLQTKLQEDELKKNYIKAVNDGLLKVFSKMGISTLQSYQG
                     AQIFEILGLNKEVVNKYFTGATSRIEGMGLDEIAREVLAKHYFAFSKKDIPADRLPAG
                     GIYQWKRKGEFHLFNPQTIHLLQHATKMNDYPTFKKYSKLVNDQSEKACTLRSLFQFK
                     KTRPSVSIDEVQAAENIYKRFATGAMSFGSISWEAHTTLAIAMNRLGGKSNTGEGGED
                     EKRYEPLSNGDSMRSAIKQVASARFGVTSLYLTEADELQIKMAQGAKPGEGGQLPGHK
                     VDEWIGKVRHATPGVGLISPPPHHDIYSIEDLAQLIYDLKNANRKSRISVKLVSKAGV
                     GTIAAGVTKAKADVVLIAGHDGGTGASPISSIKHAGLPWELGLAETHQTLVKNKLRSR
                     VVVQADGQLKTGRDIAIAALLGAEEWGIATAALVVEGCIMMRKCHMNTCPVGVATQDP
                     ELRARFKGDPDHVVNFFKMITQELREIMAELGFKTVNEMIGQADNLEVRDEIEHWKYK
                     NLDLSAVLYNEPNSIYSTLYCSEKQDHGLDDSLDWQLWEAAVPAIVAKEKISASFPIK
                     NINRTVGTILSNEITKKYRSEGLPEDTIHFKFNGTAGQSFGAFNTKGITLELEGDAND
                     YFGKGLSGAKLIIYPPKEATYTAEDNSIIGNVAFYGATSGEAFIRGKAGERFCVRNSG
                     ANVVVESVGDHGCEYMTGGRVIILGDTGRNFAAGMSGGIAWVYDVKGKFANNCNKEMV
                     DLDDCDENDKNDLYNFIQKHLQYTGSTVAKFILSDFENQF"
     assembly_gap    15161..15170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15290..>16080
                     /locus_tag="PK977_04065"
     CDS             15290..>16080
                     /locus_tag="PK977_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRF17313.1"
                     /translation="MGKPTGFKEFSRELPGRQPVEQRLKHYNEFGERYTDEKLNQQAA
                     RCMNCGVPFCHHGCPLGNVIPEFNDAVYRKDWKEAYDILSSTNNFPEFTGRICPAPCE
                     TACVLGINQPAITIEEIEKHIIEIAFEKGFVKPRRPNLRSGKKVAVVGSGPAGLAAAA
                     QLNYAGHSVTVFERDDAPGGLLRYGIPDFKLEKWVVDRRIKLMEEEGVVFKCHANVGV
                     NVSINDLLREYNAIVLAGGSTVPRDLKIPGRELNGVHFAMDFLKQ"
     assembly_gap    16081..16136
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16137..>16464
                     /gene="gltD"
                     /locus_tag="PK977_04070"
     CDS             <16137..>16464
                     /gene="gltD"
                     /locus_tag="PK977_04070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588156.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HRF17314.1"
                     /translation="IIEFELTATCKNVVVIGGGDTGSDCVGTSNRHGANSVTQFELLP
                     KPPSERNDFMPWPSYPMLLKKTSSHEEGAARQWAVATKEFIGDANGNLRSLKIVDLEW
                     KIVDGRP"
BASE COUNT         5009 a         3095 c         3765 g         4006 t
ORIGIN      
        1 ttacccagcc gggcatgacg attgtttgcg gtgacagcca tacctcaacc cacggcgcct
       61 ttggttccat cgcctttggc atcggcacca gtgaagtgga aatggtgctg gctacacaat
      121 gtctcctgca aagcaaaccc aaactgatgc gcatcaatgt agatggtgaa ttaagtaaag
      181 gtgtggtatc aaaagatatt gtgctctaca ttctgtcaaa gattactgcc agcggcgcta
      241 cgggttatgc agtagagttt gccggctctg caatccgctc actcagtatg gnnnnnnnnn
      301 ntgaccattt gcaacatgag tattgaaatg ggtggccgct gcggattgat tgcgccggat
      361 gaaactactt tccaatatat gaaaggaaga cttcatgccc ctaaagggga tttatgggag
      421 caatcattaa gcttttggaa aactctttat agcgacgagg atgccgtctt tgataaagaa
      481 tatcatttcc gtgctgaaga tattgaaccg atgatcactt atggcaccaa tcccggaatg
      541 ggcattggta tttcagaaaa tattcctaca actgatacga tcaacgataa tgacaaatca
      601 ggattagcaa aatcgctggt ttatatgggc cttcaggaag gcgctgccat caggggacag
      661 aaaatagatt atgtatttat cggcagctgt accaattcaa gaattgaaga cctgcggatg
      721 gtggcctctt ttgtgaaagg gaaaaagaag gcagacaatg tggaagtatg gatcgtaccc
      781 ggcagtaaac aggtagaagc acaggctata caggaaggga tcgataagat atttgaagaa
      841 gccgggttcc agttaagaca accgggctgc tctgcctgcc tcggtatgaa tgagacaaga
      901 tacctgccgg taaatattgc atcagcactt ctaaccgaaa ctttgaagga agacaaggcg
      961 ccggtgcgag gactttactg gcaagtccgc taacggcagc agcagcagct ataacgggca
     1021 gcataaccga tgtaagggaa ctattgaatt aaccatgaaa gcattaacga ccatacaaag
     1081 caggtttgtt ccggttaata tcgagaacat tgacaccgac cagatcatcc ctgcccgttt
     1141 tttaaaagca actacaaaag aaggttttgg taagaacctg ttccgtgact ggcgatacta
     1201 tggcgacgat gaatcaaaaa caagggctga ctttccattg aacaggtcac agtttgacgg
     1261 cgaaatatta gtagccggta aaaactttgg ttgtggttca tccagggaac atgctgcatg
     1321 ggcactcgcc gattatggtt tcagagtagt ggtgtccagt ttttttgcag acatctttcg
     1381 caacaatgca ctgaacaatg gtgtactgcc ggtgcaggtt agtgaagcat tcctgcaaag
     1441 aatattcctt caggataaca acacagaact gacggttgac ctggccagcc agtcaattac
     1501 tattaactca accggtgagc aggaaacttt tgaaataaac agttataaaa aaacatgcct
     1561 gctcaacggg tatgatgata ttgattattt aatgagcata aaagaagaca tcaaacaatt
     1621 cgaaacccgg gaagcataac agactatgaa aaaaagaatt gcagttatag aaggcgatgg
     1681 cataggcccc gaagttacca ggcaggctgt gaaagtgctg aatgcggtag cagccgcttt
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaca gtgatgccat
     1861 tttgtttggc gctattggtc atccaaaata tgataatgat cctgctgcca aagtaagacc
     1921 ggaacagggc ttattaaaac tccgcaagtc actgcagttg tatgccaata tccggcctgt
     1981 gactacgtat tcctctttgt tccatttgtc gccattgaaa agtaagaata ttgaaggtgt
     2041 tgattttatt atataccggg aactgactgg tggtatttac tttggaaaaa aggaaacggc
     2101 tgctgacgga tcacaggctt ctgacgattg tacctatagc cgggaagaaa ttgaacgnnn
     2161 nnnnnnnnca ccttgctttt cagcatgccc ggaagagaaa aaataaatta acactggtag
     2221 ataaggccaa tgtgctggag acatcaaggc tctggagaaa agttgtgcag gaactggcgc
     2281 tgcaatacag cgatgtggca gtggattata tgtttgtaga taatgcggcc atgcaaatca
     2341 tcctgaaccc gaaacagttt gacgtgatac tcacagaaaa tatgttcgga gatatcatca
     2401 gtgatgaagc cagtgtgatc agcgggtcgc tgggtttgct tccctctgca tcggttggca
     2461 aaggcgctgc attgttcgaa cctattcatg gctcttaccc gcaggcagcc ggaaaagata
     2521 ttgccaatcc caccggctca attctttctg cggccatgct gctggatcat cttgaactga
     2581 aagaagaagc agaactggtt cgtgatgccg tgaactggac actgcaaaac tcttttgtaa
     2641 caaaagatat tgacccggtt aatttttatt tcacttccac tatcggcgaa ctgatctgtg
     2701 attatataag tggcaaaatt tcaggaacgg tgaatacaga gaatatagaa ttgagaaaat
     2761 caacgatcat ctgatcaata ataactaacg atggtttgtc catcatgaaa ataattattg
     2821 cttgctgagt tctttttttg aaacggcggg tgtatattta ccagacatac atctcatgtt
     2881 gaagaacagt acatataatg ttttcacttc tgtagctaca attattattg tggtggtgat
     2941 tattatatct tcttcaaccg gaggtggtgc atgatactga actaaacaaa ctaaatataa
     3001 gcgacccgcc tccaaaaagg cgggtcgttt tttttattac ccgtcataag tcaaaacaag
     3061 caatatgtat tacaacgaaa acactattat ctatttcacc gggcagtttg taaaagctgc
     3121 agaagccaaa acggatctat acagtcagtc attgcactat ggctatgctg tgtttgaagg
     3181 catccgctcc taccctgtta acggcggctc taaaatgttt aaaggcaagg agcattatga
     3241 caggttgcgc cgttccgctg aagtgatgaa gatccctttt tcgtattcca ccgaccaact
     3301 agttgaactg acttacgaaa cattgagaag gaatggcttc actgatgctt atataagacc
     3361 gatcgtaatc tgctcaccca atatggcatt atcaaaaggt aaagaatcat acctggccat
     3421 agaatgctgg gaatggacaa atggctatat gagcaacaag atgaagatca tgacttcctc
     3481 ttaccgtcgt cccaacgccg atgcatttca tgtggatgcc aaagtaagcg gtcattatgt
     3541 gaactccata cttgcctgcc aggaagctaa agacaatggg tttgatgaag cactggtgct
     3601 tgacagcaac ggctatgtgg ccgaaagcag cggtgccaat atcttttatg agaataacgg
     3661 tgtattgtac accgctccga aaggaaatat tcttcccggt attaccaggg cgactgtttt
     3721 agaaatctgt gaggaactga atataccggt acaggaaaaa ttatttaccc cggaaaaaat
     3781 gcggggagca gatgcaggtt tcttctgtgg tactgcggca gagattgttg gactgagttc
     3841 attggataat gtaccctttg caaaagaatg ggaagacaca ctcagctatg taatacaaca
     3901 ggcttataaa tgcaaagttt tggagaaacc ctttcaactg gaacagaaag tagcataatg
     3961 atgaatgaac tgaataaata cagcaagact cttacacagg acccgacgca accggctcca
     4021 caggccatgt actatgccat tggttttaaa caagaagatt ttgataaagc acaagtgggt
     4081 attgtaagca tgggatggga tggcaatccc tgcaacatgc atttgaatga cctcgcaacc
     4141 agtgtacgta acagcgtaaa tgccactgaa ggcctattgg ggcttcgctt ttatactatt
     4201 ggtgtaagcg atggtatcag tatgggcacc gatggtatgc gctatagttt ggtaagccgt
     4261 gatgttattg ctgacagcat tgaaactaat gcaggcgcac aatactacga tgccataatt
     4321 gctattccgg gctgtgataa aaatatgccc ggtgccataa tggcgatggg aagactgaac
     4381 cgtcctgcca ttatgttgta cggaggcact attgcacccg gtcattataa aggagaagac
     4441 ctaaatattg taagtgcctt tgaagcactg ggacaaaaga tcgccggcaa tttaagtgaa
     4501 gaagatttta aaggaattgt acaacactct tgtcccggcg ccggtgcctg cggcggtatg
     4561 tacacagcta atacgatggc atctgccatt gaagcaatgg gtatgagttt gcctaattct
     4621 tcttccaatc ctgcattaag tgccgaaaaa caaagagaat gtgcggcagc cggtgaagcg
     4681 ataaaagttt tactggaaaa aaatattact cccaaagata tcatgacccn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaggccttt cgctgatgca ggatgatttc
     4861 caggaaatca gcaaccgtat accggtgctt gctgatttca agccatcggg caaatacctg
     4921 atggaagacc tgcataagca tggcggtgtt cctgctgtga tgaaatattt attgaaaaat
     4981 ggcttactcc acggcgattg tttgacagta accggtaaaa cggtggctga gaatttagca
     5041 ggtgtacctg atctcgattt tgaggcgcag aaaattattt accctctgga acagccatta
     5101 aagaaaacag gacacctgca aattctgtat ggtaacctgg ccaaagaggg cagtgtggcc
     5161 aagataagcg gcaaagaagg agaagagttt gaaggaactg cccgggtgtt tgacggcgan
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cacccggtat gccggaaatg
     5341 ctaaaaccaa ccagtgccat tattggagca gggctgggca aatcagttgc attgatcacc
     5401 gatggaaggt ttagtggcgg cacacatggt tttgtggttg gccacatcac ccccgaagca
     5461 tatgaaggtg gattgattgc attggtgaaa gatgatgaca tcatccgtat tgatgcaaag
     5521 aaaaataaga taacactgaa agtaaataag gaagaaatca aaaagcggag gaagaaatgg
     5581 aagcagcctg cattaaaagc aagcagcggc atactttata aatatgcccg gatggtaaaa
     5641 ccggcaagcg aaggttgcgt gacggatgaa gagtgaaaat tgattcattg atcattaacg
     5701 aaattgttat ggatacactg gaattaaaaa aagcaaagcc acagccaaaa gctttaaaaa
     5761 acttaagcgg ttcagttgcg gtactggaag cccttcttgc cgaagaggtt actaccatct
     5821 ttggttatcc cggcggcgcc atcatgccaa tctatgatgc gctgtatgat tacaaggaca
     5881 agctgaaaca tattttagtc cgtcatgaac aaggtgctat tcatgcagca cagggctttg
     5941 ccagggtaag cggaaaaaca ggagttgttt ttgctaccag cggacccggt gccacaaacc
     6001 tggtaactgg tttagcggat gccatgattg actctactcc tgttgtgtgc attaccgggc
     6061 aggtatttgc gcatttgctg ggtacagatg cgttccagga aacagatgta attaatatca
     6121 ccactccggt taccaaatgg aattaccagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnntgat ataaccaaga atgcacagtt gcagcttttc gaatatcaag gatatacaaa
     6301 atgtgaacac atccgcagct atcgccccaa accgatcgta cgaaaagaat atgtggaggc
     6361 agcggctaaa cttatcaata aagcggaacg tccttttgtc gtttttggcc agggtgtgat
     6421 tcttggcaaa gcagaaaaag aattcaaaca attcatagag aaagcaggta tccctgctgc
     6481 atggaccatc atgggtatga gtgccatacc cactgaccat ccgctgggag caggtatgct
     6541 gggcatgcat ggcaattatg ggcctaatgt attaaccaat gaatgtgatg tgctgattgc
     6601 aataggtatg cgtttcgatg acagggtaac cggccgcctg gataaatatg caaagcaggc
     6661 aaaggtcatt cacctggata ttgacccggc agaaattgat aagaatgtaa aagccgatgt
     6721 accggtatgg ggagattgca aggaaacact tcccatgctt actgcattgg tagaacaaaa
     6781 agtatatccg cagtggctgc aaaagtttaa tgactacaaa caaaaagaag aagagaaagt
     6841 aataaaggat gaactgaacc caaccggaga agtaatgacc atgggagaag ttatcaaaac
     6901 actcaatgaa ctgacaaatg gcgatgccat tattgtaacc gatgtaggtc agcaccagat
     6961 ggtagcttgc cgctatgcga aatacaatca cagcaaaagt aatatcacca gcggtggcct
     7021 gggaacaatg gggtttgcct taccggcagc tattggtgct gcatacggag ataccagccg
     7081 aaccacaatt gccctcattg gcgatggcgg tttccagatg accttgcagg aactaggcac
     7141 catcatgcag tttaagccca atgtaaaaat catcatcctc aacaaccagt tccttggcat
     7201 ggttcggcag tggcagcaac tgtttcatga aaaaagatat tcgttcgtgg atataactag
     7261 ccctgatttt gtgcaggtgg ccaaaggctt tggcattccg ggtaagagca tttcaaaaag
     7321 aggagaattg aaagattcat taaagcaaat gctggaacag gatggaagct ttttactgga
     7381 aataatggtt gggaaagaaa acaacgtatt cccgatggta ccgcagggaa gaggcgtggc
     7441 agaatgtgta ttaagtaaag aagaaattta aatcccgaca gcggttgaaa actatgtcgg
     7501 cggttagaaa aaataaatgc tatgactaaa aaagaattta caataacagt ctatacagaa
     7561 aaccaggttg gtttactcaa ccgcattgcg atcatcttct cccggagaaa gatcaatgta
     7621 gagagcctga atacttctcc caccgaagtg gatagtgtcc atcgctttac aattgttatc
     7681 aatgaaacgg aagaagtggt aagaaagctt tgcaggcaaa ttgaaaagca ggtagaagtg
     7741 ctcaaggcct actataacac agaacatgag atcgtttggc aggaactggc attatacaaa
     7801 gtgccaacag atgttatagc tgcagaggta aaggtagaaa gattgctaag ggagaacggt
     7861 gcccataccg tagtaataag aaaagactat accgtatttg cagtagcagg ccaccgggaa
     7921 gaaacagaag cattgattaa agtattggag ccgtttggcc tgattgaatt cgtcaggacc
     7981 gggcgggtgg ccattatcaa aggcagcagc ggctttcatg aaagactcaa agaatttgaa
     8041 caaacagaac ctggtgagga agtcctcgaa aatgagtacc tggataaaaa cgaagaagta
     8101 tttacgatgt aatcaataag aaataaaaat aaacaactac aaaaaagatc attatggcaa
     8161 aactaaattt tggcggcgtt gaagaaaacg tagtaaccag ggaagaattc ccgcttagca
     8221 aagcacagga agtgctgaaa aacgaaacag tggctgttat cggctacggc gtacaaggtc
     8281 caggacaggc attgaaccaa agagacaacg gaatcaacgt tatcgttgga cagcggaaaa
     8341 acagcaaaac ctgggataag gccattgcag atgggtttgt tcccggcgaa acattgtttg
     8401 aaatagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnaattgcc ttgtggccaa ctgtacagaa atacttaacg ccgggtaagg cattgtattt
     8521 ctcacatggc tttggagtta cattcaacga acaaaccggt atcgttccgc ccaaagaggt
     8581 agatgtgttt ttagtagcgc ctaaaggaag cggcacctca ctgagaagaa tgtttttaca
     8641 gggtcgcgga cttaacagct cttatgccat ttaccaggat gcaacaggca gggcttttga
     8701 aagagtaatt gcattaggta ttgctgttgg cagcggctac ttatttgaaa ctgattttaa
     8761 aaaagaagtt tactctgaac tgaccggcga aagaggaaca ctgatgggcg ccatacaggg
     8821 atttgctgca caataccagg tattgcggga aagaggccac tctccttctg aagcatttaa
     8881 tgaaacggta gaagagctga cgcagtcact aatgccgctg gtagcagaaa atggtatgga
     8941 ctggatgtac gccaactgct ccactactgc tcagcggggt gcactggatt ggtggaaaaa
     9001 attcagggat gccactgcac ctgtctttaa tgaattgtat gacagtgtag ctgcaggcaa
     9061 agaatcgcag cgatctattg aaagcaacag taagactgat tacagggaaa aactggagga
     9121 agaactaaaa gaactgcgtg aaagtgaatt atggcaggcc ggcaaaacag taagaagtct
     9181 cagcccggaa aatcaaacta aaaattcttn nnnnnnnnna aaaaaatttc acctgtaaaa
     9241 ccggctgctt ctatcaaaga ggtatttagc aaactggtaa aacaacctga atcaacggaa
     9301 gaaaaacaat atttagtata atgatagcat caacgcataa tgtgcagtta gattttgcag
     9361 cagcatcatt gcgactgaaa aaagtggtga acagaacgcc gctcagcttc aatcacaatc
     9421 tgtccaggaa ataccagtgc aatgtatacc tgaaaagaga agacctgcag gtggtacgtt
     9481 cctataaatt gagaggagct tacaatatgc tgagtacttt atcctcagaa caactggaaa
     9541 aaggagtagt ctgtgccagt gcaggtaacc atgctcaggg ctttgcctat agctgcaaaa
     9601 agctaaatac caggggagta atttatatgc cggtcataac tccccggcaa aaaatttcac
     9661 aaactaaaat gtttggtgaa ggaaatatcg agattaaact atcaggcgac acctttgatg
     9721 actgtgctgc tgctgctaaa aaatttactg aggagaatag tatgacgttc attccccctt
     9781 ttgatgatta tcgaatcatt gagggacagg gaacagttgg aactgaaata ctggaggatc
     9841 tctctgatat tgatttcctt ttcattccgg ttgggggcgg cgggctaagt gccggtgttg
     9901 gcacctattt caaaacctac tcacccaaca caaaaataat tggcattgaa ccggaaggcg
     9961 caccctccat gtctgaagcc ttaaaaaacg gttacccggt aacactggaa acaataaatc
    10021 gttttgttga tggtgccgcc gtaaaaagag ccggtgagat cacttttggc atttgcatgg
    10081 aagtattaga cgatatgcac ctggttgccg aaggaaaggt atgtactact atattaaaat
    10141 tgtataacga agatgcaata gtggtagagc ctgccggggc tttatccatt gcagcgcttg
    10201 atgactatgc aggcgaaata aaaggaaaga atgtagtttg tattgtaagc ggaagtaata
    10261 atgatataga caggatgcag gagataaagg aaagaagcct gcaatatgaa ggtctcaagc
    10321 attattttct catcagtttt gcccaacggc ccggtgcctt gaaagaattt gtgaacaatg
    10381 tattgggtcc tgaagatgac ataacccgtt ttgaatacat gcaaaagacc aacaaagaaa
    10441 atggaccggc actggtgggt gtggagttgc aaagcaggtc agactacgat ctgttggtta
    10501 agaaaatgaa agaatcaaac atagattttg cagagataaa caaaaacgac cagttgttcg
    10561 gatacctcat ttaatccact tgttctatat caatttgtgg aaaattgaat tagcgatcac
    10621 cttatccgct gaagacaatt ttgtattttc agatgtattg cttgctgttg aattgaactt
    10681 aacaaagttt cgatcattta atattttatg caatggcagg caaccaggga ctttacgatc
    10741 cttcttttga acatgatgca tgtggtattg gcttcgtagc gaatatcaaa ggacataaat
    10801 cacatcagca tatttcggat gcgctgaccg tattggaaaa tatggaacat cgcggcgcct
    10861 gtggttgcga agcaaatacc ggtgacggcg ccggcattat gattcagacc ccgcatgaat
    10921 ttttctttga tgagtgcctg aaactcggtg tacaccttcc ctcctttgga aaatatgctg
    10981 ccggaatggt tttcttccca aaagaaataa gactaaggga ggaatgccgg gatatactga
    11041 accgctcagc agaaaaatta ggtcttgaaa ttctgaccta cagaaaagta ccggtaaaca
    11101 cagaaagtat cggccctaca gctttaagtg tagagcctga aatggaacaa gtattcattg
    11161 cttgtcccga tcatatttcc aaccccgatg attttgaaag aaaattattt gtactgcgta
    11221 attatgcttc tcatcttatc aacagtacag tacgtaaaga tgaaattggt ttttatatcg
    11281 catcgctttc ttataaaaca attgtataca aaggtcagct taccagtcac caggtccggc
    11341 aatatttccc tgacctgagt aacaaaagag tggtcagttc ttttggctta gtacactnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tccttcgtgg aaactagccc agccctttcg
    11461 ctttattgcc cacaatggcg agatcaatac actgcaggga aatttaaact ggctcaaaac
    11521 cagtgaaaaa gggtttacta ctccttactt cagtaaagaa gagatggaga tgctgctacc
    11581 tatcgttaca caaggacaaa gcgattctgc ctgtttggac aatatgattg aactgcttac
    11641 actcacaggc cgttccctgc cacatgtaat gatgatgctg ataccggagg catgggacgg
    11701 aaacgaagaa atggatccgg taaagaaagc attttatgag tttcattctt catttatgga
    11761 accatgggac ggaccagcct ctatttcttt tactgatgga aaaatcatcg gcgctacttt
    11821 agacagaaat ggcttacgtc catcaagata ctgcgtcaca gatgatgacc gtgttataat
    11881 ggcttctgaa acaggaacac tgcctgttga ccctgccagc atcattgaaa aagggagatt
    11941 gcaaccggga aagatgtttg ttgtggacat ggaaaaaggc aagatcatca gcgacgaaga
    12001 cctgaaaaaa gaaatctgtt cacaaaaacc atatagtgac tggctgaata aatataagat
    12061 caggctggag gagttacctg aaccacgaat tatgttcact cacctggaac atgaccaggt
    12121 attcaaatac cagaaggctt ttggttattc aaccgaagat cttgacacta tcattgctcc
    12181 catggctgtc gatggaaaag agcccatcgg ttcaatgggt actgatgtgc ccttagcaat
    12241 actcagtgat cagccgcagc accttagtag ttatttcaaa caattatttg cacaggtaac
    12301 caatccgcct attgatccaa tcagagagag actagtaatg tcattgtcaa catttgcagg
    12361 caataatgga aacctgcttg ttgaagatcc gttaacttgt catagtgttg ccttgaaaca
    12421 tcctgtgctt actaattacg aactggaaaa aatcaggagt attgataccg ggctttttca
    12481 gtcgaaaacc attcaatgtt atttcagggc tgatggcaaa ccggggtctc tcaaaaaggg
    12541 acttgaacga atatgtgaat atgcagtgca tgccgtagaa gatggtttta aggttttaat
    12601 actacaggat agggcaattg attcaaacca tgcccctatc ccatccttac tttctacatc
    12661 ggctattcac catcacctta taagaaaagg aatgcggggt caagtgggca ttattgtgga
    12721 agcaggcgat gtttgggaag tacaccattt tgcctgctta attggttttg gagcaactgc
    12781 tgttaatccc tacctggcac tctcttccat aagagattta aaagaaaccg gcaaattgca
    12841 aacaaagttg caggaagatg agctgaagaa gaactatatc aaagcggtga atgatggctt
    12901 gctgaaagtg ttttccaaaa tgggtatctc tactttacag tcttaccagg gtgcacagat
    12961 ttttgagata ctgggcttga ataaagaagt tgtaaataaa tatttcaccg gggccacttc
    13021 aagaattgaa ggaatggggc tcgacgagat tgcccgtgaa gtgttggcca aacattactt
    13081 tgcttttagc aagaaggata ttccggccga caggctgcct gccggcggta tctaccaatg
    13141 gaaaagaaaa ggcgagttcc atttatttaa cccgcaaacc atacacctgt tgcagcatgc
    13201 aacaaaaatg aatgactacc caacattcaa aaaatattca aaactggtca acgatcaaag
    13261 tgaaaaagcc tgcacactgc gcagcttgtt ccagtttaaa aagacaagac cttctgtaag
    13321 tattgatgag gtacaagctg ctgaaaacat atacaaacgt ttcgccaccg gcgccatgag
    13381 tttcggttct atctcatggg aagcacacac tactttggcc attgctatga accgccttgg
    13441 cggaaaatca aataccggtg aaggtggtga agatgaaaaa agatacgaac ccctgtcaaa
    13501 cggagattct atgcgcagcg ccatcaaaca ggtggcatct gcaaggtttg gtgtaaccag
    13561 tttatactta acagaggcag atgaactaca aataaaaatg gcacaaggcg ccaagcccgg
    13621 cgaaggaggt cagttgcccg gacataaagt agatgaatgg attggaaaag tacgtcatgc
    13681 cactccggga gttggattaa tttcacctcc accacatcat gatatttatt caattgagga
    13741 tttagcacaa ctgatatacg atttaaaaaa tgccaatcgt aaatcaagaa tcagtgtaaa
    13801 actggtaagc aaagccggtg taggtactat tgcagccggt gttactaaag caaaagcaga
    13861 tgttgtatta attgccggtc atgatggcgg tacaggcgcc tcaccgatca gttccatcaa
    13921 acatgccggg ttaccatggg aactgggcct ggcagaaact caccagacac tggtgaaaaa
    13981 caaattaaga agccgtgtgg tagtgcaggc tgacggtcaa ttaaaaacag gaagggatat
    14041 tgccattgct gcattgctcg gtgctgaaga atggggcatc gctacagcag ctttggtagt
    14101 agaaggctgt ataatgatgc gtaaatgcca tatgaatacc tgtcctgttg gtgttgctac
    14161 ccaggatcct gagctaaggg caaggttcaa aggtgacccg gaccatgtag tgaacttttt
    14221 caaaatgatc acacaggaat taagagagat aatggctgag ctgggcttta aaacagtaaa
    14281 tgaaatgatt ggccaggcag ataatcttga agtaagagat gaaatagaac attggaaata
    14341 taaaaatctt gacttgtctg ctgtattgta taatgaacct aattccattt acagcactct
    14401 ttattgcagt gagaagcagg atcatggtct tgatgacagc cttgactggc aattatggga
    14461 agctgctgtt ccggctattg ttgcaaaaga aaaaatatca gcttctttcc caatcaagaa
    14521 tatcaaccgt actgtaggaa ctattctatc taatgaaata acaaagaagt accgttcgga
    14581 gggattacct gaagacacta ttcattttaa attcaacgga acagccgggc aaagttttgg
    14641 cgctttcaat acaaaaggaa ttacactgga actggaaggt gatgccaatg actatttcgg
    14701 gaagggatta agcggggcaa aattgatcat atatcctcca aaagaggcta cctacacagc
    14761 agaagataac agtattattg ggaatgtagc attttacgga gccacatcag gagaagcgtt
    14821 tatcagggga aaggccggcg aacgattttg tgtaagaaat tctggcgcta atgtagtagt
    14881 ggagtcagtt ggcgaccatg gttgtgaata tatgacaggt ggccgtgtaa tcattttggg
    14941 tgacaccggg agaaattttg ctgcaggtat gagcggtggc atcgcatggg tgtacgatgt
    15001 gaaaggaaaa tttgccaata actgcaataa agaaatggtg gaccttgatg attgtgatga
    15061 gaacgacaag aatgatttgt acaatttcat tcaaaagcat ttacagtata cgggcagtac
    15121 cgtcgcaaaa ttcattctga gtgattttga aaaccagttc nnnnnnnnnn ttgtgaaagt
    15181 atttccaaag gattataaga aagtattgca tgaaaaaata aatactgttg aaacaagcgt
    15241 ttgaagcaat ccttttcaac caattaacaa atcaactgat aaacttctca tgggcaaacc
    15301 aacaggattt aaagaatttt ccagagagct tcccggcagg cagccagtgg agcagcggct
    15361 gaaacattat aatgaatttg gtgaacgcta tactgatgaa aaactcaatc agcaggcagc
    15421 ccgctgcatg aactgtggtg tacctttttg tcaccacggt tgtccgctgg ggaatgtgat
    15481 acctgaattt aatgatgctg tttatcgtaa agactggaaa gaagcttatg atattctttc
    15541 ttcaacgaat aatttcccgg aattcaccgg ccgtatctgc cccgctccct gcgaaactgc
    15601 ttgtgtactg ggtatcaatc agccggccat tactattgaa gaaattgaaa aacacatcat
    15661 tgaaatagct tttgaaaaag gatttgtaaa accccgcagg cccaatctcc gttcagggaa
    15721 aaaagtagct gtagttggtt cgggaccagc agggcttgcc gctgctgcac agctgaatta
    15781 tgccggccat tcagtaacgg tttttgaaag agatgatgca ccgggaggat tattgcgcta
    15841 tggtattcct gatttcaaac tggaaaaatg ggtggttgac agaagaataa aactaatgga
    15901 agaagaaggt gtagtgttta agtgtcatgc caacgttggg gttaatgtca gcattaatga
    15961 tttactccgt gagtataacg caattgtttt ggctgggggc agcactgttc cccgtgattt
    16021 gaaaatccct ggcagagaat tgaacggtgt tcattttgcc atggacttcc tgaagcaaaa
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatt
    16141 attgagtttg aattaactgc tacctgcaaa aatgtagtgg tgattggagg tggtgatacc
    16201 ggtagtgatt gtgtgggcac atccaatcgt catggtgcaa actccgttac acagtttgaa
    16261 ctgctgccca agcccccatc agagagaaat gattttatgc cctggccttc ttatcccatg
    16321 ctcttaaaaa aaactagctc acatgaagaa ggagcagcaa gacaatgggc agttgctaca
    16381 aaagagttta taggagatgc aaacgggaac ctgagatctc tcaaaattgt tgatcttgaa
    16441 tggaagattg tcgatgggcg acct
//
LOCUS       DAORLS010000031        16272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1919_length_16272_cov_2.432077, whole genome shotgun sequence.
ACCESSION   DAORLS010000031 DAORLS010000000
VERSION     DAORLS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16272
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1919_length_16272_cov_2.432077"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>378
                     /locus_tag="PK977_04075"
     CDS             <1..>378
                     /locus_tag="PK977_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17315.1"
                     /translation="KVEQVSERKRPQTPQPPFPYRSEDVVYNNSDNSLRYGATITIPE
                     GKGPFPAAVLITGSGAQDRDETLFGHKPFAVIADHLTKNWYIVLRVDDRGTGKSTGDF
                     SQSTTEDFAKDVSNSVDYLLTRPE"
     assembly_gap    379..395
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <396..1085
                     /locus_tag="PK977_04080"
     CDS             <396..1085
                     /locus_tag="PK977_04080"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HRF17316.1"
                     /translation="KKKIGLIGHSEGGMIAPMVATSRKDIDFIVLLAAPGVRITELMA
                     EQNAAILRSSGLSIEAVSEAKKLFKLLVSTIRGTSDSATALQQVSMAMENWKAKQADS
                     ILIALELSKPEKRKEYISVMTNQLQGPWFRYFMNFDPKPYLEQLNCKVLALNGSRDIQ
                     VISSQNLPGIEAALKRSKAKAVTLKELPGLNHLFQSCNACTIDEYGLLEETFSPAALL
                     DMSNWLNKNVK"
     gene            1162..1704
                     /locus_tag="PK977_04085"
     CDS             1162..1704
                     /locus_tag="PK977_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HRF17317.1"
                     /translation="MISDFVKGKKKFNYHFNIQKGIALHRAIDSFTDEHPATKEAKEI
                     FRPYYRLYSGAFVDVVYDHFLAADEQEFTEESLHRFAQEVYHTLHMHQPLMPERFARM
                     FPYMKEQNWLFHYRSQWGTEKSLGGLVRRAAYLTESETAFGLFQQHYQRLQHYYRLFW
                     TDIKPFARKKFEELLNSDIH"
     gene            1765..2325
                     /locus_tag="PK977_04090"
     CDS             1765..2325
                     /locus_tag="PK977_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF17318.1"
                     /translation="MKKAFFLFPALCFSVMILAQSNVPPVDKSPLDISYYPANYPVLK
                     IQDKITEPLAARVLYSRPQKAGRPIFGGLVKYGELWRLGANEATEIEFFKNVHLGKKK
                     INKGRYTLYAVANESTWTIIVNKETDTWGSFKYDAKKDVTRVVVTAQKTDTVVESMSM
                     YFEKTTSGFNLIIAWDNIKTAVPFTF"
     gene            2335..2979
                     /locus_tag="PK977_04095"
     CDS             2335..2979
                     /locus_tag="PK977_04095"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF17319.1"
                     /translation="MKITSCCFSLVLSIIFISCNSGKDEKKESNTKKTTPDSINRILP
                     AKPVNPYATVDISPMDMAYFPVDYPKLKMAGQLTATQPHARVIYSRPHLEGRHIFKEV
                     LKYGEPWRMGANEATELELYSDALIQNKKIKAGRYILYCIPQPKEWTIVFNSNTDTWG
                     LQQDASKDVARFTVPVKETTNSLEYYTMIFEQAEKGTNLLMAWDNVEVRLPLSF"
     assembly_gap    3137..3146
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3147..4931
                     /gene="asnB"
                     /locus_tag="PK977_04100"
     CDS             <3147..4931
                     /gene="asnB"
                     /locus_tag="PK977_04100"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRF17320.1"
                     /translation="KSMTDSIAHRGPDGEGHWQNAEETVLFGHRRLSIIDLSNAGDQP
                     MHYLNRYSIVHNGEIYNYVELKEELQKKGYSFRSQTDTEVILAAYDCWQQECVDHFDG
                     MFAFAIWDDKDKTLFAARDRFGEKPFFYHYEGNNLFFASEMKALWAAGIEKTPNQKML
                     FNFITIGYTDNPAKREETFYENIFRLPPASSLVYSPVKNELLIEQYWDIDLQAENKTI
                     TDGEAVERFSELLTTSVKRRLRSDVAVGTSLSGGLDSSSIAAFIHELLPHNNYCCFTA
                     SFPDMENNDLFHAKNVAKNFGLQQHIAECTASGLIKDWEKLCYHQEEPMGSASAYAQY
                     KVYERAKEQGIKVLLDGQGADEILAGYHKYYKWYWQELFRKRKLKKSKEIFWAKEIGT
                     NENFDYRNMIAAYFPAFASIVMERQYLLKALKQEDLTREFVQLQSKEAYYTPPEHFDL
                     NGVLYFNTMTHGLEELLRYADRNSMAHGREIRLPFLSHELVEFIFSLPSHFKIRNGWT
                     KWLLRETVKEKLPHSSVWRRDKVGFEPPQKKWMENGSVQDAMQEAKQKLVSEKILKES
                     VLNKKITPSAAYEAENYDWRYWSASLLF"
     gene            complement(4920..5489)
                     /locus_tag="PK977_04105"
     CDS             complement(4920..5489)
                     /locus_tag="PK977_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17321.1"
                     /translation="MKIQWFLFVLLIGLVCMSMTQCPQCIKGRLKVDDNAKQWLPYRG
                     KDSITFISSSGVISKMKCSYADVTNTYQNFECDDTYEADSVGFTLEIKPSDSLYLTCN
                     MNSPSWLCFRTMNMDSAFISGCNVLNGPITEMRKSFTTYTLNNFRYSDVKLVNAYPGT
                     NPDFDSLFFARNYGVVSFKYKSTWYYLKQ"
     gene            5594..>6565
                     /locus_tag="PK977_04110"
     CDS             5594..>6565
                     /locus_tag="PK977_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF17322.1"
                     /translation="MKAATKFIHAGAHPDPSTGAIMTPVYQTSTYVQEAPGVNKGFEY
                     ARSQNPTRKALEEALAVIENGKFGLAFSSGVAATDAVIKLLAPGDEVVCANDMYGGTY
                     RLFTKVFERFGIVFKYVDTTDVKNVAAAVTEKTKLVWLETPTNPLMNVTDIAAVAAIT
                     RSKNILLAVDNTFASPALQNPLDLGADIVMHSVTKYLGGHSDVIQGALIMNDADLREK
                     LYFIQKSCGAVPGPMDCFLVLRGIKTLGVRMKAHCENGARIAHWLRNHPKVGKVYWPG
                     FEDHPGYAVAKKQMKGFGGMISFELKNDSVEEAKRVLSSTHLFSLAES"
     assembly_gap    6566..6602
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6606..6734
                     /locus_tag="PK977_04115"
     CDS             6606..6734
                     /locus_tag="PK977_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRF17323.1"
                     /translation="MTHASIPREERIKNGLSDSLIRLSVGIEDADDLIEDLAKAIG"
     gene            6742..>7179
                     /locus_tag="PK977_04120"
     CDS             6742..>7179
                     /locus_tag="PK977_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HRF17324.1"
                     /translation="MNKTHLKQLLNKKVDFYNQPSFIVTDPISVPHLFTKKQDIEIAG
                     FFAAIFAWGNRTTIINKAKELMHLMDMAPYEFVRSHDEKDLKRLLGFKHRTFNDTDLL
                     YFVDFFKHHYRSNKSLETAFTRHGYTAEKILTGFHQYFFSLEHI"
     assembly_gap    7180..7193
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7194..7525
                     /locus_tag="PK977_04125"
     CDS             <7194..7525
                     /locus_tag="PK977_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRF17325.1"
                     /translation="TRKHIATPERNSTCKRLNMYLRWMVRRDDKGVDFGIWKDISPAQ
                     LICPLDVHVARVARQLGILKRKQNDWETALELTEYLRTLDKDDPVKYDFALFGLGAIE
                     PKIAVLK"
     gene            7531..7851
                     /locus_tag="PK977_04130"
     CDS             7531..7851
                     /locus_tag="PK977_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17326.1"
                     /translation="MENHSLTEILNKDLGLAVSPQLSLEEIRSTVANQVNHLINTDFE
                     KLVSLLYRIDVSEPKMRALLQHKQGEDSAEHIADLIIERQLQKIESWKKFSNKKDISD
                     EEKW"
     gene            complement(7867..8361)
                     /locus_tag="PK977_04135"
     CDS             complement(7867..8361)
                     /locus_tag="PK977_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17327.1"
                     /translation="MASYLRKELSAEKRGNLQLITFGAPAAGNTAFVKDMEEKYPAAE
                     RYTTDKDIATVFPDMDKVREIAHITGLDSVLQLSKLSIPGINKLKTSDLLDIAGEILK
                     ATKVINETNRYVQSQRHLRLLTVDAAAVPADTSYTAESLFNKAYQFHKVDRYADLLGV
                     KPLE"
     gene            complement(8382..8897)
                     /locus_tag="PK977_04140"
     CDS             complement(8382..8897)
                     /locus_tag="PK977_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17328.1"
                     /translation="MIKKLIQPVLFIALFFVSCNTQQKQKEKSSFLSAKEVVRLNQVV
                     YKSSIAESLSRYSPEFEAVFIPEAINGNFAFIAKKKETEQYALVIRGSVIEFSNEGFQ
                     NFVMQDFNIFTIKAWPYTDTIQQAYVSNGAWIGFQNLLQLRDKSSGLTIKSSLNKRFQ
                     LTPAWLLQDIV"
     gene            complement(8964..9632)
                     /locus_tag="PK977_04145"
     CDS             complement(8964..9632)
                     /locus_tag="PK977_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF17329.1"
                     /translation="MHFEKIHNKGQARLFKNDFLEALTKAHPLVIWGMYIPILSYIIY
                     LAATKYNYSTGTILLVFFGGMFFWSLFEYIAHRFIFHWVPKTPTATKIVYTLHGNHHH
                     YPRDRQRLFMPPVPSLIISSTLFGLMYLIMGTYAFMFFPGFLLGYLMYGTMHYAIHAW
                     NPPFKWMKGLWRNHHLHHYKDEHMGYGVSSTLWDHVFGTMFDLKKEKEDKEKVQELMF
                     EKKK"
     gene            complement(9686..>9872)
                     /locus_tag="PK977_04150"
     CDS             complement(9686..>9872)
                     /locus_tag="PK977_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17330.1"
                     /translation="QSIIQNEQSKPAGLNGFILLMHIGTDPKRSDKFYKYLPKLIQYL
                     KAKGYQFQRVDSLLKTD"
     assembly_gap    9873..9933
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9934..>10268)
                     /locus_tag="PK977_04155"
     CDS             complement(<9934..>10268)
                     /locus_tag="PK977_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF17331.1"
                     /translation="FTGRFYRNKEFSKVIQQLRKDGHYLGAHSDQHLLYCDWNKRDSL
                     LVTKKQFRFDLADNYTEMTRHHIDVQKATFFLPPYEWYNDSIAAWTKELQLQLINYTP
                     GTLSHADYT"
     assembly_gap    10269..10475
                     /estimated_length=207
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10476..>11849)
                     /locus_tag="PK977_04160"
     CDS             complement(<10476..>11849)
                     /locus_tag="PK977_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRF17332.1"
                     /translation="NPFLKDSCHTHDGYVLYGEKAGIKDSTHIDVVGGWHDASDYLQY
                     VPTSANATYHLLAAYRDFPTVFTDEKSANGLDGKNGQPDVLDEARWGIDWLMKMNPRR
                     FIMFNQLGDDRDHMGMRIPKEDTNYYGKGLERPVYFVNGEPQQRGKFMNTTTGVASTA
                     GKYASVFALAHFLYERDNLFRTTDKYKGYYAKAFDAYQYGLSKPGYTQTVSVRSPYIY
                     AEENWTDDMELAAVSIHTMVNNTPMGGDNDYYTSAAYNFALKEKITPWLGADTAKHYQ
                     WYPFINLGHYELAKQLNGNQKNTIIGYYKEGIQRVWNKAKQNAFYRGIPFIWCSNNLT
                     TAFAIQCYWYKQLTGDKQFNELEQANFDWLFGCNPWGTSMVYGLPSWGDTPVDPHSAF
                     THIKQYPIDGGLIDGPVYTSIYKGLIGITLYQPDEYAAFQSDLAVYHDDYGDYSTNEP
                     TMDGTASL"
     assembly_gap    11850..11949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11950..12256)
                     /locus_tag="PK977_04165"
     CDS             complement(<11950..12256)
                     /locus_tag="PK977_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HRF17333.1"
                     /translation="MKKWLFILLLPVLSFNKKEEPSSWIRINQLGYTPDGVKVAVWCS
                     KEEKELVNWELVYADSKKIAAKGKAGKAFGVYGPFKQTYRLNFSHFKKPGRYYLQAGG
                     "
     gene            12310..13320
                     /locus_tag="PK977_04170"
     CDS             12310..13320
                     /locus_tag="PK977_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRF17334.1"
                     /translation="MKLVSYLKDEHEQLGVLINGIVYSMDVLHPDLPNSMNMFLNYWE
                     DSYPIAQAGELMLREGTRTSNKGVPLSELQLLAPVPFPNSCRDGYAFRQHVAAARRNR
                     KVDMIPEFDQYPIFYFTNHHSIQGPGEIRCMPDHFEKLDFELEAAIVICKHGRNIRAE
                     EADEYIGGLMIMNDMSARRLQMEEMLLNLGPAKGKDFSTVIGPWLVTLDELQPFEIPA
                     KENHTGKSWNLRMQCWVNDKQVSDGNLGDMDWTFAEIIERASYGVDLYPGDVIGSGTV
                     GTGCFLELNGTGKLNDPNYPEQWLQAGDVVEMEIDCLGKLSNTIVAEETDWSILRQKK
                     NS"
     gene            13326..14423
                     /locus_tag="PK977_04175"
     CDS             13326..14423
                     /locus_tag="PK977_04175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF17335.1"
                     /translation="MKKTGIIISLSFLLFSFKLMSQEKIAARIDSLFSSLLKAGEPGG
                     AVLVAKGDNIILSKGYGIADINSKEPITPHTIFNTGSVSKTFVSNAILILEQRQQLSL
                     DDNLLQYFPDFKNKGIAAKVKIRHLLSHVSGLPDNREVSKNPVFYLTADDTQNFAPVQ
                     QTDTLVFEPGSRYQYSNPAFNGLALIIEKVTGSKWQDFIRKEIFAPAGMTESDITDGA
                     HPETGVAHAYAKEKGKFKELDYGEEPTFCAAGNGGIWCSVIDLHRYYLALQKPAFLDA
                     DIIGNSIEVKKFEQWKSEKEPNNGYSWFITKTVNGLKRIGHTGSQGGFRADFEWVPEK
                     KIVICLLFNIPHDVSLLTKKIETILVEEKIL"
     gene            14425..15306
                     /locus_tag="PK977_04180"
     CDS             14425..15306
                     /locus_tag="PK977_04180"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRF17336.1"
                     /translation="MIIDLKEIKPAEKQYYLQHLVAPRPICFASTIDKNGNVNLSPFS
                     FYNLFSSNPPIVVFSPSRRVRDNTTKHTLENVLEVPEVVINMVTYDMVQQQSLASCEF
                     PKEVNEFTKAGFTEEPATLIQPPMVKESKAKMECRVLEVKPLGTEGGAGNLVICEVLR
                     IHLADELLDENMKIDQRKIHHVARLGGDWYCVVNENSLFKVPKPNTQLGIGVDALPEH
                     IRRSNILTGNDLGQLGNVHELPVITPSFDDPHLKQIIQYYSINPDEMERELHIYAQKL
                     LKEGNTDAAWQVLLAGA"
     gene            15368..16261
                     /locus_tag="PK977_04185"
     CDS             15368..16261
                     /locus_tag="PK977_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17337.1"
                     /translation="MQNIRTSRLLLIPGITFLLFSCVSQKKFVAAKSDLTKENETLTS
                     YESKLTSLDEQRAKKQQQNELADTVDLQIRKFINKTNSEIDTLIDKNKVLINGNLVSK
                     DDLNRIMKAIASTKLASQLINKKILFLEDLIKTNMVVKLDQDVLFEPGSYKVAPAVIA
                     NIGKLFEPAAKEIDKFASKYQDFTLSLVISIKGYADATTINEGSSLYNNLKARLALSN
                     SNPDAKELNKELSRARAEEVANLFKQYAKGRGDNGLYTKNIFYIYEGKGEQFPDPKIT
                     NYSMDDPRRRIVLLFWSIFPD"
BASE COUNT         4895 a         3242 c         3377 g         4312 t
ORIGIN      
        1 aaagtggagc aggtttcaga gagaaaaaga ccacaaacac cccaaccgcc ctttccatac
       61 agaagcgaag atgttgttta caacaattcg gataactctt tacgttatgg cgctaccatt
      121 accataccgg aaggaaaagg ccctttcccg gctgctgtgc taattaccgg atcaggggca
      181 caggacagag atgaaacttt attcggccat aaaccttttg ccgtgatcgc agaccacctg
      241 accaaaaact ggtacattgt tttgcgggtt gatgaccggg gaacaggcaa aagtaccggt
      301 gactttagcc agtcaactac ggaagatttt gcaaaagatg taagcaatag tgttgattac
      361 ctgcttacaa ggccggaann nnnnnnnnnn nnnnnaaaaa gaaaatcgga ttgattggtc
      421 atagtgaggg gggaatgata gcaccgatgg tggccaccag cagaaaagat attgacttca
      481 tagtactgct ggctgcaccg ggggtacgaa ttacagaatt gatggcagaa caaaatgctg
      541 ccattctccg atcttcgggt ttaagtattg aagcagttag tgaagcaaaa aagttgttca
      601 agctgttggt atcaacaata cggggcacat cggactcagc tactgctctt cagcaggtat
      661 ctatggccat ggaaaactgg aaagcaaaac aagctgattc catactgata gcccttgaac
      721 tatccaaacc cgaaaaaaga aaagaataca ttagtgtaat gacgaatcaa ctgcagggac
      781 cgtggttcag atactttatg aattttgatc ccaagccata cctggagcaa cttaactgta
      841 aggtgcttgc cctaaatggg agcagggata tacaggttat atcttctcaa aatcttccgg
      901 gcattgaagc agcattgaaa cgaagcaaag caaaagcagt aacattaaaa gaactcccgg
      961 ggcttaatca tttgttccag agctgtaatg catgcactat tgatgagtat ggcctgctgg
     1021 aagaaacctt ttcgcctgcg gcattactgg atatgagtaa ctggttgaat aaaaatgtaa
     1081 aataagaaac atccttacac catcaactac ctggcccacg catacctgtc ctttgatcac
     1141 cccgaaatac tggtgggaaa catgatcagc gattttgtca aaggcaaaaa gaaattcaat
     1201 taccatttca acatacaaaa aggtattgcc ctgcaccggg ctattgacag ctttaccgat
     1261 gagcatcctg ccacaaaaga agcaaaagaa atttttcgtc cgtattatcg gctgtacagc
     1321 ggtgcatttg tagatgtagt gtatgaccat tttctcgcag cagatgaaca agagtttaca
     1381 gaagagtcat tacatcgttt tgcacaggaa gtttatcata ccctgcacat gcatcagccg
     1441 ttgatgccag aacggtttgc ccgaatgttt ccttatatga aagagcaaaa ctggcttttt
     1501 cattaccgga gccaatgggg gactgagaaa agccttggag ggctagttcg aagagcggcc
     1561 tatcttactg agagtgaaac agctttcggt ttattccagc agcattacca acgtttgcag
     1621 cattactacc gtcttttctg gaccgatata aagccatttg cgaggaaaaa gtttgaagaa
     1681 cttcttaata gtgatattca ttaatcctta aatggtaact accggaagat taattcgttt
     1741 tacatttgta aatacctcat ttgtatgaaa aaagcttttt ttctgttccc ggcactttgc
     1801 ttttctgtaa tgatacttgc acaaagtaat gtaccgcctg ttgacaaatc accgcttgat
     1861 atcagttatt atcctgccaa ttatcctgtt cttaaaatac aggataaaat cacagaaccg
     1921 ctggctgcaa gagtactgta cagccggcct caaaaagcgg gcaggcctat tttcggaggc
     1981 ctggtgaaat acggcgaatt atggagattg ggtgccaacg aagccacaga gattgaattc
     2041 ttcaaaaacg ttcacttagg aaagaaaaag ataaataaag gaagatatac actctatgcg
     2101 gtagccaatg aaagcacctg gaccatcatt gtaaataaag aaactgacac atggggttcg
     2161 tttaagtatg atgcaaaaaa agacgtgaca agagtggtag ttactgcaca aaagacagat
     2221 acagttgtgg aatcaatgtc aatgtatttt gaaaaaacaa caagcggttt taatctcatc
     2281 attgcatggg acaatataaa aactgccgta ccatttacct tttaaacctg ttgtatgaag
     2341 attacctcct gctgcttttc tttggtgcta tctatcattt ttatttcctg taattccggt
     2401 aaggatgaaa agaaagaaag taacacaaaa aaaacaacac cggatagtat caaccgtatt
     2461 ctgcctgcca aaccggtaaa tccgtatgcc actgtagata tatcgcccat ggatatggcc
     2521 tactttcctg ttgattaccc caagctgaaa atggcagggc aactaactgc cacccaacca
     2581 catgcaagag tgatttatag ccgccctcac ctggaaggcc gtcatatttt caaagaagta
     2641 ttgaaatatg gtgagccctg gagaatgggt gccaatgaag ctactgaact ggaactctac
     2701 agcgatgcct taatccaaaa caaaaagata aaggcaggcc ggtatatact ttattgtatt
     2761 ccgcagccca aagaatggac cattgttttt aacagcaata cggatacatg gggcctacag
     2821 caggatgcat caaaagatgt ggcccggttc actgttcctg taaaagaaac aacaaattca
     2881 ctggagtatt acaccatgat ctttgaacag gcagaaaaag gaactaattt gctgatggca
     2941 tgggataatg tggaagtaag gctacccctc tccttctaac aaattcggct tcctcgcaaa
     3001 tgttttggaa attggtattt ctaatttgaa tatttgcctg ttatgtgtgg catagcaggt
     3061 atcatacaat ctaccccatc ccgttttagc attacccatc tgcaaaaaat gacccatgct
     3121 ttagcccatc ggggacnnnn nnnnnnaagt cgatgactga ctctattgcg caccgtggac
     3181 ctgatggaga aggacattgg cagaatgcag aagaaaccgt tttatttggt caccgacgtc
     3241 tttccattat tgacctgagt aatgccggcg atcagcccat gcattacctg aaccgctaca
     3301 gcattgtgca taatggagag atttacaact atgttgaatt aaaagaagag ttacagaaaa
     3361 aaggttattc cttccgttca caaacagata ccgaagtgat tctggcagca tatgattgct
     3421 ggcagcagga atgtgtggat cattttgacg gcatgtttgc ctttgccatc tgggatgata
     3481 aagacaaaac gctgtttgct gcaagagacc gttttggcga gaaaccattt ttctatcact
     3541 atgagggaaa caatttgttt tttgcctcag agatgaaagc attatgggca gccggcattg
     3601 aaaaaacgcc aaatcaaaaa atgcttttca atttcattac gatcggttat accgataacc
     3661 cggcaaaaag agaagagacg ttttacgaaa atattttccg cctgcccccc gcatcttcac
     3721 tggtatactc accggtaaag aatgaattac taatagaaca atactgggat attgacctgc
     3781 aggctgaaaa taaaacaata accgatggcg aagcggttga aagattctcg gaattactta
     3841 caacttctgt aaaaagaaga ttacgcagcg atgtggcggt aggcacatcc ttaagcgggg
     3901 gactggacag ttcatctatt gctgctttta tccatgagct gttgccacac aataattatt
     3961 gttgttttac tgcaagtttt cctgacatgg aaaacaatga tctttttcat gcgaaaaatg
     4021 ttgcgaagaa ttttggcctg caacaacaca ttgccgaatg taccgcttcc ggtttaatca
     4081 aagactggga aaagctatgt tatcaccagg aagagccaat gggttctgcc agcgcctatg
     4141 cacaatacaa agtatatgaa agggcaaagg aacaaggtat aaaagtattg ctggacggac
     4201 agggagcaga tgaaatactt gcaggttatc ataaatacta taagtggtac tggcaggaac
     4261 tttttcgcaa acgaaaactg aaaaaaagca aagaaatttt ttgggcaaaa gaaatcggga
     4321 ccaatgaaaa ctttgattac cgcaatatga tcgctgccta ttttcctgct ttcgccagta
     4381 tcgtgatgga acggcaatat cttttaaagg cattaaaaca ggaagacctc accagggaat
     4441 ttgttcagct gcaaagcaaa gaagcttatt atactccacc tgaacatttt gacctgaacg
     4501 gcgtacttta tttcaatacc atgacccatg gccttgaaga attgttgaga tatgctgaca
     4561 gaaattccat ggcacatggc agggaaatca ggttgccttt tctttctcat gagttagtag
     4621 aatttatttt ctccctgcct tctcatttta agatccgtaa tggatggacc aaatggctgc
     4681 ttcgggaaac tgtaaaggaa aaattacccc actcatctgt atggcgaaga gataaggtgg
     4741 gatttgagcc tccgcagaag aaatggatgg agaacggatc ggtgcaggat gctatgcagg
     4801 aagcaaaaca aaaactggtt agtgaaaaaa tattaaagga aagtgtattg aataaaaaaa
     4861 ttactccgtc ggcagcttat gaggcggaga attatgactg gagatactgg tctgcctcat
     4921 tattgtttta aataatacca ggtgctttta tatttgaaag aaacaacacc ataattcctt
     4981 gcaaaaaaca aggagtcaaa atccggattc gttcctggat aagcattcac aagttttaca
     5041 tctgagtatc taaaattgtt aagcgtataa gttgtaaatg attttctcat ttcagtaata
     5101 ggtccattca gcacattaca cccggaaata aatgctgaat ccatgttcat tgtacgaaaa
     5161 cacagccagg aaggagaatt catattacat gtaagatata aactgtctga cggctttatt
     5221 tccaatgtaa atccaacact atctgcttca taagtgtcat cacattcaaa attctggtat
     5281 gtatttgtta cgtctgcata gctgcatttc attttactga taaccccgga agacgaaatg
     5341 aatgttatgg aatctttgcc acggtaggga agccactgtt tggcgttatc gtctactttt
     5401 aatcttcctt taatacactg cgggcactga gtcattgaca tacaaaccag cccaatcagc
     5461 aaaacaaata agaaccactg aattttcata tccctctctt ttaataggta ttaaatgtac
     5521 tgttttttga gattcacttt taaagacaaa aggagcattt atctttgctc tttactacta
     5581 ataagcaaca aaaatgaaag cagcaactaa gttcatacat gcaggtgcac accctgatcc
     5641 ctctaccggt gctattatga cacccgttta ccaaacctca acttatgtgc aggaagcgcc
     5701 gggagtgaat aaaggctttg aatatgcccg cagccagaac cccacccgta aagcactgga
     5761 agaagcactg gcagtgattg aaaacggaaa atttggttta gccttcagca gcggtgtagc
     5821 cgctacagat gccgtgatca agttgctggc ccccggtgac gaagtggtat gtgccaatga
     5881 tatgtatggt ggcacctacc gtttattcac taaagtgttt gagcgtttcg gaatagtttt
     5941 caaatacgtt gatacaactg atgttaaaaa tgtggcagcc gctgtgacgg aaaaaacaaa
     6001 actcgtctgg ctggaaacac ctaccaatcc gctgatgaat gtaacagata ttgctgcagt
     6061 cgcagccatt acaagatcca aaaacatttt attagcggtt gacaacactt ttgcatctcc
     6121 tgccctgcaa aacccgttag acttgggtgc tgatattgta atgcattctg taaccaagta
     6181 cctgggagga cacagtgatg tgatacaggg agcattgatc atgaatgatg cagacttgag
     6241 ggaaaaatta tacttcatcc aaaaaagctg cggtgctgtg cccggaccga tggattgttt
     6301 tttagtgctg cggggtatca aaaccttagg agttcgaatg aaagctcatt gtgagaatgg
     6361 tgcaaggatt gctcactggc tccggaatca ccccaaagtc ggtaaagttt actggccggg
     6421 ctttgaagac catccgggat atgcggtagc caaaaaacaa atgaaaggat ttggcggcat
     6481 gatttctttt gagctgaaga acgacagcgt agaagaagct aaaagagtgc tgtcctccac
     6541 tcacctgttt tcattagcag aaagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnagtatgac gcatgcttct ataccaagag aagaaagaat taaaaacgga ctgtccgatt
     6661 cgcttatccg gctgagtgtc ggcattgaag atgcagatga tttgattgaa gacctggcaa
     6721 aggctatcgg ataattgaac aatgaataaa acgcatctaa aacaattact gaataagaag
     6781 gttgatttct acaatcaacc ttcttttatt gtgacagatc ccatttccgt tcctcacctg
     6841 ttcaccaaaa aacaggatat agagatcgct ggttttttcg ctgccatatt tgcctggggc
     6901 aaccgcacta ccatcatcaa taaagcaaaa gagctgatgc acctgatgga tatggcacct
     6961 tatgaatttg tcaggagcca tgatgaaaaa gatttgaaaa gattactggg atttaagcac
     7021 cgtacattta atgacacaga cctgctttat tttgttgact ttttcaaaca tcattaccgc
     7081 agtaataaaa gccttgaaac tgcctttacc cggcatggct atacagccga aaaaatactg
     7141 acgggttttc atcaatattt tttctcatta gaacatatan nnnnnnnnnn nnngaacccg
     7201 aaaacatatt gccacccctg aaagaaactc tacctgcaaa agactgaata tgtacctccg
     7261 ctggatggta aggcgtgatg ataaaggtgt tgattttggt atctggaaag atatttcacc
     7321 ggcacagctc atttgccccc ttgatgtaca tgtagcaagg gtggcccggc agctgggtat
     7381 cctgaaaaga aaacaaaatg actgggagac cgccttagag ctaactgaat acttacgaac
     7441 tttggataag gatgatcccg tgaagtacga tttcgctttg tttggactgg gagctattga
     7501 accaaaaatt gcagtattaa aatgattggc atggaaaacc attcgcttac agaaatactt
     7561 aataaagacc tgggtcttgc cgtgtcaccc caattaagtt tggaagagat acggtctacc
     7621 gtagccaatc aagtcaatca tcttatcaat actgattttg aaaaactggt ttccctgctt
     7681 taccgcattg atgtgagtga gccaaaaatg agagccttat tgcaacacaa acagggagaa
     7741 gacagcgcag aacatattgc agatctcatc atagaaaggc agttacaaaa aattgaaagc
     7801 tggaaaaaat tcagcaacaa aaaagatatt tctgacgaag aaaaatggta aggcgtaata
     7861 ccccgtttac tctagcggtt taacacctaa aagatctgca tacctgtcta ctttgtgaaa
     7921 ctggtatgcc ttgttgaaca aactttctgc tgtataagaa gtatctgcag gaactgcagc
     7981 agcatccacg gttagtaaac ggaggtgccg ctggctttga acataccggt ttgtttcatt
     8041 gatcactttg gttgctttca gaatttcacc ggcaatatcc agaaggtcgc ttgtttttag
     8101 tttattgata ccgggaatac tcagcttgct taattgtaat acactatcaa gcccggttat
     8161 atgagcaatc tctcttacct tatccatatc cggaaaaacg gtcgcaatat ccttatccgt
     8221 tgtatatcgt tccgcagcag gatatttttc ttccatatct tttacaaaag ctgtattacc
     8281 ggcagccgga gccccaaaag tgatcagctg taagtttcct cttttttccg ctgacagttc
     8341 ctttctcagg tagctagcca ttgggtaggc caggttaccg cctaaactat gtcctgtaat
     8401 aaccaggctg gcgtcaactg gaatcttttg ttcaacgaac tcttaattgt taaaccggaa
     8461 gatttgtctc tgagttgcaa caggttttga aagccgatcc aggcaccatt gctgacataa
     8521 gcctgctgaa tagtatcagt atagggccat gctttaatgg taaaaatgtt gaaatcctgc
     8581 attacaaaat tctgaaagcc ttcattactg aactcaatca ctgatccccg gataaccagt
     8641 gcatactgtt ctgtttcttt ctttttggca ataaatgcaa agtttccgtt gatagcttcc
     8701 ggaataaaca ccgcttcaaa ttccggagag taccgggata aggattctgc aatagatgat
     8761 ttgtacacta cctggtttag ccggactact tctttggccg aaagaaacga tgatttttcc
     8821 ttttgtttct gttgggtatt acaggaaaca aaaaacaatg caatgaataa aactggctgt
     8881 attaactttt ttatcatgat attaaaaaaa gagaaccaca caggattagc tatgtggttc
     8941 tgaaattact tatttattaa taactatttc tttttctcaa acatcagctc ctgcactttc
     9001 tctttgtctt ccttttcttt cttcaaatcg aacatggtgc cgaatacatg atcccataac
     9061 gtagagctta caccatatcc catgtgctca tctttgtagt gatgcaggtg atgattgcgc
     9121 caaaggccct tcatccattt gaaaggcggg ttccatgcat gtatggcata atgcatagtt
     9181 ccatacatca ggtagcccag caagaagccc gggaagaaca tgaacgcata ggtccccata
     9241 atcaggtaca tcaacccaaa cagtgttgat gagatgataa ggctaggcac cggcggcata
     9301 aagaggcgct gcctgtctct cggataatga tggtgattgc cgtgcagagt ataaacgatc
     9361 ttcgttgcgg ttggcgtctt gggaacccaa tgaaatataa accggtgtgc tatgtattca
     9421 aatagtgacc aaaagaacat gcctccaaaa aacaccagca ggatagtgcc ggtggaataa
     9481 ttatactttg tagcggcgag gtaaataata taactgagga taggtatgta cataccccag
     9541 atcaccagcg gatgggcttt ggttagcgcc tccaaaaaat catttttgaa taaacgggcc
     9601 tggcccttat tatgaatctt ttcgaaatgc atggcgcaaa gataaggaaa ggagaatggg
     9661 tgctacaaag gctgttgaac tgattttaat cagttttcaa taatgaatca acccgctgaa
     9721 actgatagcc ttttgccttt aaatactgta tcaactttgg caaatattta taaaatttat
     9781 cgcttcgctt gggatcagtg ccgatatgca tcaaaagtat aaacccgttt agcccggcag
     9841 gttttgattg ctcattctga atgatagact ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtataa tcagcatggc tgagggttcc
     9961 gggggtgtaa ttgatcagtt gtaactgtaa ttcttttgtc caggccgcaa tactgtcatt
    10021 gtaccactca taaggcggta aaaaaaacgt tgctttctgc acatcaatat ggtgccgggt
    10081 catttcggtg tagttatccg ccaggtcaaa ccggaattgt tttttggtga ccagcaggct
    10141 gtctcttttg ttccagtcgc aatacaacaa atgctgatct gaatgagcac cgagataatg
    10201 gccatccttt cttaactgct gaatgacctt actgaattcc ttattacgat aaaatctccc
    10261 ggtgaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaggg aagcggtacc atccatcgta
    10501 ggttcattag tgctgtaatc gccataatca tcatgataca cagcaaggtc actttgaaag
    10561 gctgcatatt cgtccggctg ataaagggta atgccaataa gtcctttgta tatgctggta
    10621 taaacgggcc cgtcaattaa tccgccgtca atcgggtatt gtttgatatg ggtaaatgca
    10681 gaatgcggat caacaggcgt atcaccccat gacggcaacc catataccat cgaagtgccc
    10741 catggattgc agccaaacaa ccagtcgaaa ttggcttgtt ccagttcatt aaattgctta
    10801 tcgccggtta gttgtttata ccaataacat tgaatggcaa aagctgtagt aaggttatta
    10861 ctgcaccaga taaaaggaat gccacggtag aaagcatttt gtttggcctt gttccatacc
    10921 cgctggatgc cttctttgta atagccaata attgtatttt tctgatttcc gtttaattgt
    10981 ttagctaatt cgtaatgacc caaattgata aaagggtacc attgataatg tttcgccgta
    11041 tcggcgccta accaaggagt tattttttct tttagcgcaa aattatatgc agcagatgtg
    11101 taataatcgt tatcgccgcc cattggtgta ttattcacca ttgtatgaat agaaactgca
    11161 gccaattcca tgtcatcagt ccagttttct tctgcataga tataaggcga tcttacggaa
    11221 actgtttggg tatatccggg tttagacaat ccatactgat atgcatcaaa tgctttggca
    11281 taataccctt tgtatttatc tgtagttcga aacaggttgt ctctttcgta caggaaatga
    11341 gccagtgcaa atacactggc atattttccg gctgtgctgg ctacaccggt agtcgtattc
    11401 ataaacttac ctcgttgttg cggttcgccg tttacaaagt aaactggtct ttctaatcct
    11461 ttgccatagt agttggtatc ttcttttggt attcgcatgc ccatatgatc acggtcatca
    11521 cccaattgat taaacatgat aaacctgcgg ggattcattt tcataagcca gtcaatcccc
    11581 caacgagcct catccagtac atcgggttgc ccgtttttac cgtctaaacc attggctgat
    11641 ttttcatcag taaaaacagt tggaaaatcc ctgtaagcag ccagtagatg gtaagtggca
    11701 ttcgctgatg tgggcacata ctgtaaataa tcgcttgcat catgccagcc gcccaccaca
    11761 tcaatatgag tactgtcttt gatacctgct ttttcaccat acaatacata gccgtcatgg
    11821 gtatggcaac tgtcttttaa aaaaggattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnc accgcctgcc tgcaaataat agcggccggg ttttttaaaa tgagaaaagt
    12001 tgagccggta agtttgttta aacggcccgt aaacaccgaa agcttttccg gccttacctt
    12061 ttgccgctat tttctttgaa tccgcatata ccaattccca attaaccaat tccttttcct
    12121 ccttactgca ccacacggct actttaaccc catcgggtgt atatcccagt tgattgatac
    12181 gtatccagct tgaaggttcc tcttttttat taaacgagag aaccggtaaa agcagaatga
    12241 acagccattt tttcattacc acaatttaca accaacctcg ctaacttagc agccattaat
    12301 tattctccga tgaaattagt aagctacctg aaagatgaac atgaacaact gggagtattg
    12361 atcaacggga tagtatacag catggatgta ctgcaccccg atctgcccaa cagcatgaat
    12421 atgtttttaa attactggga agacagctat cccattgcgc aggctggtga actgatgctg
    12481 agggaaggca cccggacaag caataagggt gtgcccttaa gtgaacttca actgctggca
    12541 ccagtacctt ttcccaattc ctgccgtgac ggatatgcat ttcgtcagca tgtagctgct
    12601 gctaggcgca accgaaaagt ggatatgata ccggagtttg atcagtaccc gatcttttat
    12661 ttcaccaatc atcacagtat acagggaccg ggtgagattc gttgcatgcc cgatcatttt
    12721 gaaaaactgg attttgaact ggaagccgcc attgtaattt gcaaacatgg cagaaatata
    12781 agggctgaag aagcagatga atacatcggg ggcctgatga ttatgaatga tatgagtgcc
    12841 agaaggcttc agatggagga aatgctgcta aacctgggtc ccgccaaggg aaaagatttt
    12901 tctactgtca ttggaccctg gctggtaaca ctggacgaac tgcagccttt cgaaattccg
    12961 gccaaagaaa accataccgg caaaagctgg aacctgcgga tgcagtgttg ggtaaatgat
    13021 aagcaggtaa gtgatggcaa cctgggtgat atggactgga cctttgctga gatcattgaa
    13081 agggcatcat atggtgttga tttatatccc ggtgatgtga ttggcagcgg cactgtggga
    13141 actggctgtt ttcttgaatt gaatggtacc ggtaaactca acgaccccaa ttaccctgaa
    13201 caatggctgc aagccggtga cgttgtagaa atggaaattg attgtttggg gaaattgagt
    13261 aacaccattg tggctgaaga aacagactgg agtattctcc gtcaaaagaa aaacagctga
    13321 acatcatgaa aaagacagga ataatcatat ctctgtcgtt tttgcttttt tcattcaaac
    13381 tgatgagcca ggaaaaaatt gcagcaagga ttgattcatt gttttcttcc ctgctaaaag
    13441 caggagaacc gggtggtgcc gtattggttg ccaaaggcga caatattatt ttgagcaagg
    13501 gatacggaat agctgatata aacagcaaag aaccgattac ccctcatacc attttcaata
    13561 ccgggtctgt aagcaaaaca tttgtaagca atgctatcct catcctcgaa cagaggcagc
    13621 agctcagcct ggatgataac ctgctacaat attttcctga ttttaagaac aagggtattg
    13681 cagcaaaagt aaagatcagg cacctgctca gccatgtaag tggcttacct gacaacaggg
    13741 aagtaagcaa gaaccctgtt ttttatttaa ccgctgatga tacccagaac tttgctcctg
    13801 ttcaacaaac agatacattg gtttttgaac cgggcagccg ataccaatac agtaatcctg
    13861 ctttcaacgg tcttgccctg atcatcgaaa aagtaacggg tagtaagtgg caggatttca
    13921 tccgcaaaga aatttttgcc ccggcaggaa tgactgaaag cgatatcaca gatggtgctc
    13981 atccggaaac cggtgtggca catgcctatg caaaagaaaa agggaaattc aaagaactgg
    14041 attacggtga agaacccact ttctgtgctg caggcaacgg aggaatttgg tgttcggtta
    14101 ttgacttgca ccgctattac cttgctttac agaaaccagc tttcctggat gcagatatta
    14161 ttggtaacag tatagaagta aaaaaatttg agcaatggaa aagtgagaaa gagcctaaca
    14221 atggttacag ctggtttatt acaaaaacgg ttaatggatt aaaacgtatt ggacataccg
    14281 gcagccaggg aggcttccgg gccgattttg aatgggtgcc tgaaaagaaa attgtgatct
    14341 gccttttgtt caatattccg catgatgtga gcttactgac aaagaaaatc gaaactattt
    14401 tggtagaaga aaaaatatta tagaatgatt attgatctga aagaaataaa accggccgaa
    14461 aaacagtatt acctgcaaca tttagtggct ccccgtccta tctgctttgc ttctacaata
    14521 gataaaaacg gaaatgtaaa cctgagtcca ttcagctttt acaatctttt ttcatccaat
    14581 ccgccaattg ttgttttttc tccgtcaaga agggttagag acaacactac aaagcataca
    14641 ttagagaatg tgctggaggt accggaagta gtgatcaata tggttaccta tgatatggta
    14701 cagcaacaat cgctggccag ctgcgaattc ccaaaagaag tgaatgaatt caccaaagcc
    14761 ggatttacag aagaaccggc caccctgata caaccaccca tggtgaaaga aagcaaggct
    14821 aagatggagt gcagggtgct ggaagtaaaa ccattgggca ctgagggtgg tgccggcaac
    14881 ctggtgatct gtgaagtatt gcgcattcat ttagctgatg agctattgga tgaaaacatg
    14941 aagattgacc agagaaaaat tcatcatgtg gcgaggcttg gcggcgactg gtactgtgtt
    15001 gttaatgaaa acagtttatt taaagtgccc aaacccaata cacaacttgg cataggtgtg
    15061 gatgcattac ctgaacatat ccggcgaagt aatattctta ccggcaatga tcttggccag
    15121 ttgggcaatg tgcatgaatt gccggtgata actccttcat ttgatgaccc gcacctcaaa
    15181 caaatcattc aatactacag cattaacccg gatgagatgg aaagggaact gcatatatat
    15241 gcacaaaaac tactgaaaga aggaaataca gatgctgcct ggcaggtttt gttagcaggc
    15301 gcctgatctg ttgttttact gcccggactt ttctaacttt aatagctcac cattaaaatg
    15361 ttcttctatg caaaacataa gaacatcccg cctgctgctt atcccgggaa ttacattttt
    15421 acttttttct tgtgtatcac aaaaaaaatt tgtggcagcc aaaagcgatt taaccaaaga
    15481 aaacgaaaca cttacatctt atgagtcgaa gctgacttcg ctggatgagc aacgagctaa
    15541 aaaacagcaa caaaacgaac tggctgatac cgtggatctg cagatccgga aattcatcaa
    15601 taaaacaaat tcagagattg atacgcttat tgacaaaaac aaagtgctga tcaacggcaa
    15661 cctggtgagc aaggatgacc tgaaccgaat catgaaagct attgcttcca caaagcttgc
    15721 atctcaactg atcaacaaaa agatattatt tcttgaagac ctgataaaaa ccaatatggt
    15781 ggtaaagctg gaccaggatg tactttttga acccggcagc tataaagttg ctcctgctgt
    15841 gattgccaac attggaaaat tatttgaacc cgcagcaaaa gaaattgaca agtttgcttc
    15901 gaaataccag gacttcactc tttcactggt catcagtatc aagggttatg cagatgcaac
    15961 aacgatcaat gaaggctctt cgttatataa caatttaaaa gcaaggctgg cactttctaa
    16021 ttcaaacccg gacgccaaag agcttaataa agaattatcc cgagccaggg ctgaagaagt
    16081 agcaaatctt ttcaaacaat atgcaaaagg aagaggggat aacggtcttt atacaaaaaa
    16141 tattttctac atctatgaag gaaaaggaga gcaattcccc gatccaaaga tcactaatta
    16201 cagtatggat gatccgcgga gaaggatcgt acttctgttc tggagtattt ttccggattg
    16261 atcagttact gc
//
LOCUS       DAORLS010000032        15829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_1993_length_15829_cov_1.952263, whole genome shotgun sequence.
ACCESSION   DAORLS010000032 DAORLS010000000
VERSION     DAORLS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15829
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1993_length_15829_cov_1.952263"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PK977_04190"
     CDS             complement(<1..459)
                     /locus_tag="PK977_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17338.1"
                     /translation="MKTARHLILSIVVLLAAVAARSQGVSVADTIKIPIAYKPAQNRE
                     LRHDYIDNEQKSILAADGIADNRFSPTSNDEINFLVTQSLVKKVDALQYLIETDSILD
                     HRLKVKYLSGLEYVLKYFSQNWKIKSDKKVNPASLPAIINAYEQAMMKDRK"
     gene            685..1362
                     /locus_tag="PK977_04195"
     CDS             685..1362
                     /locus_tag="PK977_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17339.1"
                     /translation="MENKARKVLIADDEPDILEILKYNLSNEGYEVITAKDGDEALEK
                     ARRTNPDLVILDVMMPKKTGVEVCQLLRSQAAFKETLIIFLTAVNDEGTQIKGLETGA
                     DDYLSKPISPKVFLSRVNALFRRLNKTEARVLTIENMTIDPERFIVKVGEEEIVLAKK
                     EFELLYLLAQKPGRVFLRNEILNQVWGNDVIVGDRTIDVHIRKIRQKLGIDCITTVKG
                     VGYKFEL"
     gene            1426..2475
                     /locus_tag="PK977_04200"
     CDS             1426..2475
                     /locus_tag="PK977_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF17340.1"
                     /translation="MFSTKNISPRQLSAFTALMLAVPISVLIGYVEKSWWIGLVCFAI
                     ILAGSYFLISFMLERFIYRKIKLIYKFIYKTKATKREETYYKYVLPQKSIDEVREDVE
                     AWATKNEEELEMLRQNEQFRKEFLQNLSHEFKTTVFAIQGYVDTLLSGAMEKPEINKK
                     FLEKTAKNVDRLTHLLNDLDEISRLERGELVLYKENFVIQDLVKDVYESLSVKTEPLH
                     IYCTIKKGCESPLTVFADKEKIRQVLTNLVENSIKYGKQNGSITASMYKTDGKHILIE
                     ISDDGIGIPEKHLLRIFERFYRTEEGRSIDVSGSGLGLAICKHIIEAHGQTIHVRSTE
                     AVGTTVGFTLDSRRD"
     gene            complement(2472..3161)
                     /locus_tag="PK977_04205"
     CDS             complement(2472..3161)
                     /locus_tag="PK977_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF17341.1"
                     /translation="MNKAEILIIDDEPQIRRLLEITLHSNGYAVSLAATAKDGLIMAA
                     NHPPDLILLDIGLPDKNGQEVLKSLRQWFTNPVIILSVQDDEDDIISALDSGANDYLA
                     KPFRTGELLARIRSALRSVVMEDSDPVIRGHDLEIDLTTRTVKKNKELVKLTITEYSL
                     LALLVRNEGKVLTHQYLLRAVWGPGFINQSQYLRVFIAQLRKKIETDSNRPEYLLTES
                     GVGYRFVISQP"
     gene            complement(3158..>3721)
                     /locus_tag="PK977_04210"
     CDS             complement(3158..>3721)
                     /locus_tag="PK977_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF17342.1"
                     /translation="LAEIAKASVRLNQQVENLLNMSRIESGFLQPKMNWCDISEIIYA
                     VVRRVEEDGALQQIKIDVPQQLPLFKLDKGMFEQVLFNLLSNAVRHTPADASIRLGAS
                     FDQYLLVTVEDTGKGLPEEEIPFVFDKFYRLKNTTVSGTGLGLSIVKGFTEAMGGSVQ
                     VQNKTGGGAIFTIKLPAEISSIKKISG"
     assembly_gap    3722..3922
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3923..4235)
                     /locus_tag="PK977_04215"
     CDS             complement(<3923..4235)
                     /locus_tag="PK977_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRF17343.1"
                     /translation="MKDFLQSKPGIRWQYFYSVLIVITVSLLCYGLSPYLGYRVIALI
                     LLLAVSIIAISFEIFPVLLAAALSAFIWDFFFIPPHFTIHVASTEDAILLIMYFIIAM
                     VN"
     gene            4390..>6224
                     /locus_tag="PK977_04220"
     CDS             4390..>6224
                     /locus_tag="PK977_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRF17344.1"
                     /translation="MSISHNKASAAGLLIALGIIFGDIGTSPLYVLNAIMNGKTVSEE
                     LIAGALSCIIWTLTLQTTIKYVILTLRADNKGEGGIFSLFALVRRRKKWLVIPAMAGG
                     AALLADGMITPPISVTSAIEGLKQVPALAHISQWTVVYIVIGILTVLFFIQQFGTAFI
                     GRFFGPVMLVWFVMLASLGVVQLFNYLPVLKAFNPYYAYNLLSNYPQGFFILGGVFLC
                     TTGAEALYSDLGHCGKWNIRYSWVFVKTCLILNYLGQGAWLLHYHNGEMISASMIEQG
                     FNPFYGIMPHWFMYFGISIATAATIIASQALISGSFTLVSEAMRLNLWPKLRINYPTE
                     TKGQLYIPAVNFLLFAGCIGIVLYFQKASNMEAAYGLAITATMIATSVLFANYLVLHR
                     TKSVLVYLYLIIYLAIEFSFFYANLDKFPHGGYVTLIVGGLLFIIMYDWFRARKIKNR
                     YVEFVRLEHYVPKIQELSNDKSVPKYATHLVYMTSADNPKEIEHKIIYSILNRKPKRA
                     DIYWLIHVETLDDPYTTEYKVTHIIPNDIIRIDFRLGFRIQPRINLLFRKVVEDLVTN
                     KEVNITSRYESLEKNNVTGDFSFVVMEKYLSQDNELPFIERLIMK"
     assembly_gap    6225..6236
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6606..7103
                     /locus_tag="PK977_04225"
     CDS             6606..7103
                     /locus_tag="PK977_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRF17345.1"
                     /translation="MAKRKDYISWDEYFMGVALLSAKRSKDPGTQVGACIVNQKNKIV
                     GAGYNGLPAGCDDDEFPWDKTGEFLQTKYPYVCHAELNAILNNIGMDLHGCRIYTALF
                     PCNECSKAIIQAGITEVIYLSDKYAGNETSVASKLMLDKAKVKYRKVRSSIEKIELSF
                     NAGNV"
     assembly_gap    7290..7348
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7349..>7657)
                     /locus_tag="PK977_04230"
     CDS             complement(<7349..>7657)
                     /locus_tag="PK977_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRF17346.1"
                     /translation="KRLLRILSELLDMSQVESGKIQLLIREVQPGELVEKAVLSVLNT
                     AKQKNITIRKEVADSLPLIRADADKTTWVLNNFLTNAIRHSAENAEIIIGAQQKENQV
                     "
     assembly_gap    7658..7695
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7696..>8394)
                     /locus_tag="PK977_04235"
     CDS             complement(<7696..>8394)
                     /locus_tag="PK977_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRF17347.1"
                     /translation="IVTVCITICLLLGFTFQFNFPSLVASPVAKLTEGIKAVANKKYS
                     ERIHLNRKDEFGALAEAFNSMAERLDEYEHSSLAKILFEKQRAEAVINSFKDASIGMD
                     KNGTVLFANQQALQLLNLNEPDIVGAEKETVMKRNDLFRFLLNEHSNSPFKIVVDGKE
                     NYFFKEVMELSHEGQKTGHVIVLKNITPFKELDVAKTNFIATISHELKTPLASSDFTL
                     KLLEDERIGSLTSEQ"
     assembly_gap    8395..8406
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8407..8837)
                     /locus_tag="PK977_04240"
     CDS             complement(<8407..8837)
                     /locus_tag="PK977_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRF17348.1"
                     /translation="MKLKTKIALGGLFLFVLLITVTVVSFFYFNKVSQDAKNIVQDNY
                     ESLNYSREMLRVLDSRDDSASAIVVFEQNLRLQENNITETGERELTESLRQNFNKVKD
                     KGQTGLLAINIRSNINDIMKVNLKAIDTKNQAAQVAADKAKT"
     gene            complement(8843..>9277)
                     /locus_tag="PK977_04245"
     CDS             complement(8843..>9277)
                     /locus_tag="PK977_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein KdpD"
                     /protein_id="HRF17349.1"
                     /translation="VDYEVTSKERPFRHEHFLACISTNHEIAQRIIRKTGRLASYYNS
                     QWYVLYVQTPKEATDKIPLAAQRHLINNLKTATELGAEVLQVKHTNIAKAIMETVTEK
                     EITTVCIGKPHLNLFQVILRTNVFNQLLKTLSAREVDLIILS"
     assembly_gap    9278..9381
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9382..9964)
                     /locus_tag="PK977_04250"
     CDS             complement(<9382..9964)
                     /locus_tag="PK977_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein KdpD"
                     /protein_id="HRF17350.1"
                     /translation="MPDSEANKWLEKIQQSQKGKLKIYIGMSAGVGKSFRMLQEAHAL
                     LRNGVNVKVGYIETHGRKETEALVDGLPIIPRRKVFYKGKELDELDVQAIFLLQPDVV
                     IVDELAHTNIPGSKNEKRWQDIIDILEAGIDVITAVNIQHIESINEDVKDITGVVVTE
                     RVPDKLLQMADEVVNIDLTADELITRLKEGKIYD"
     gene            complement(9964..>10482)
                     /locus_tag="PK977_04255"
     CDS             complement(9964..>10482)
                     /locus_tag="PK977_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF17351.1"
                     /translation="RGNSLMSWGTQIQFKPSSKVLLNYGTFIGTDKPDSTRLSRFFHN
                     IYGIFNVTDKFGIIANVDIGSEEKTPGGSDKNIWYSPALILRYAFNGKLAIAGRVEHY
                     NDKNEVIITTASPNGFQTTGFSLNIDHSPFKNVVVRLEIRNLSSKDKIFLKPSGTTES
                     NTFFTSSIAVSL"
     assembly_gap    10483..10500
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10501..11048)
                     /locus_tag="PK977_04260"
     CDS             complement(<10501..11048)
                     /locus_tag="PK977_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF17352.1"
                     /translation="MKKILIGTAVLMNISLAAQDSTVNLTLSGYAEAYYTYDFNKPAD
                     NNRPGFIYSHNRHNEFNLNLGFLRVNYTAERVRANIALAAGTYMNANYAAEPGVLKNV
                     FEANASIKISKKKNLWIDAGIMPSHIGFESAVSKDCWNLTRSILADNTPYFEAGAKIS
                     YTTDDGKWFFSGMALNGWQRIT"
     gene            complement(11064..11618)
                     /locus_tag="PK977_04265"
     CDS             complement(11064..11618)
                     /locus_tag="PK977_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HRF17353.1"
                     /translation="MKKSLLISLKLTLLMIILCAVIYPLLIAGIGKATPGGGKGRTVS
                     VNGKVVGYENVGQKFTEDKYFWGRPSAVEYNAAGSGGTNKGPSNPDYLQIVQDRIDSF
                     LVHNPGVRKEEIPADLVTASGSGLDPDITPASAYVQVKRVAAIRKLAEDKVRTLVDQQ
                     ISKTILGPSVVNVLKLNIALDNLK"
     gene            complement(11629..>13041)
                     /gene="kdpB"
                     /locus_tag="PK977_04270"
     CDS             complement(11629..>13041)
                     /gene="kdpB"
                     /locus_tag="PK977_04270"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRF17354.1"
                     /translation="EGASRQKAPNEIALTILLAAFTLIFIIVCVTLKPFADYANTPIT
                     IAAFISLFVCLIPTTIGGLLSAIGIAGMDRALRANVIAKSGKAVEAAGDVDVLLLDKT
                     GTITIGNRKATNFYPANGIDKKNFIEACVLSSLSDQTPEGKSIIELAGINTKSYNTQG
                     ATFIDFTAETRSSGVNLPDGRRIRKGAFDSIQNIVIKEGNSFPKETAERVKLISSNGG
                     TPLVVSLNEKVIGVIELQDIIKPGIQERFERLRKMGVKTVMVTGDNPLTAKYIAEKAG
                     VDDFIAEAKPEDKMNYIRKEQQGGKLVAMMGDGTNDAPALAQADVGVSMNSGTQAAKE
                     AGNMVDLDNDPTKLIEVVEIGKQLLITRGTLTTFSIANDVAKYFAIVPALFIASIPAL
                     QSLNIMGLKSPESAILSAIIFNALIIPALIPLALKGVQYKPIGASALLRRNLFIYGLG
                     GIIVPFIGIKLIDMLLGLFM"
     assembly_gap    13042..13130
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13131..13687)
                     /locus_tag="PK977_04275"
     CDS             complement(<13131..13687)
                     /locus_tag="PK977_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HRF17355.1"
                     /translation="MTKHKRTVRLLEPALVGTAFRQSFIKLSPRLMVKNPVMFTVEIG
                     TAIMLTVCSWQLITDNTSEGALWYNITVFAILLLTVLFANFAEAIAEARGKAQAESLR
                     RTREETPAKKVLTVGEMYVNEIKVVSSSQLRKGDFFVCEPGDIIPMDGEIVEGIATID
                     ESAITGESAPVIREAGGDKSSVTGGT"
     gene            complement(13698..15413)
                     /gene="kdpA"
                     /locus_tag="PK977_04280"
     CDS             complement(13698..15413)
                     /gene="kdpA"
                     /locus_tag="PK977_04280"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRF17356.1"
                     /translation="MNSEILGTIIIFGATILLAWPLGKYISAVYAGNKNISDFMKPVE
                     KFFFRFCGINSAESMNWKQFLKAMLTINMLWLVYAMLLLIFQDKLPLNPDGNPGQTPD
                     LAFNTAISFLVNCNLQHYSGESGLTYFTQLFVITFLQFVSAATGMAMLVALFNGIKEK
                     TTNNLGNFWNYFVKSITRILLPLSILVAVILAFNGTPTTYDGKDTIITLQGDTVQVSR
                     GPAAGMIAIKQLGTNGGGWFGVNSAHPLENPNYITNAVETISILIIPIALVFALGYYL
                     KRIKLSNVIFAVMTFLFVIFCAINIYYETKGNPAIDQLGIAQTTGSMEGKEVRLGAAA
                     TALWSVATTSTSNGSVNGMHDSLTPISGGVVILDMMINALYGGVGVGLLNYFIFIIIA
                     VFISGLMVGRTPEFMGHKVEAREVKIAALVTLLSAFLIKGFTAFAAYLVAHHPEMGWA
                     VKPGAWLNNPSYHGFSEMLYEYTSANANNGSGFEGLGDNNIFWNVTTGIALLLGRFLP
                     IIAPIAIIGLLAKKKYIPESPGTLRTDSYTFGAMTLAVIIIVTALSYLPALALGPLAE
                     YFSLY"
     gene            complement(15504..15812)
                     /locus_tag="PK977_04285"
     CDS             complement(15504..15812)
                     /locus_tag="PK977_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17357.1"
                     /translation="MRKLLFSFKALLTRLFALLLLSLQVAGTLFGISLLKKENEFYQK
                     ENRLPVEAACPYCSLVFRQPDYCFINKISVDMLTVILLAAGLILFWLFYKSIDWFENI
                     "
BASE COUNT         4321 a         3309 c         3069 g         4597 t
ORIGIN      
        1 tttcctgtct ttcatcatcg cctgttcata tgcattaatt attgccggca ggctggccgg
       61 gttaaccttc ttgtcagact taatcttcca gttttgacta aaatacttca ggacatattc
      121 cagaccggag agatacttaa cttttagccg gtgatcaagt atgctatctg tttcgatgag
      181 gtattgcaat gcatccactt ttttgacaag gctttgcgtt accagaaagt tgatctcgtc
      241 attgctggtc gggctgaacc tgttatctgc tattccatca gcagcaagga tagatttctg
      301 ttcattatca atataatcat gccggagttc cctgttctgt gctggtttat aagcaatagg
      361 gattttgatg gtatcggcca cacttacccc ttgtgaacgg gcagcaactg ccgccagtaa
      421 cactacaatc gataagataa ggtgcctcgc tgttttcatt ataaggattg aaacgtcaaa
      481 ggtagtaagc aatgctgaca tggcattttc tgttacttct tactgataca gacgcattta
      541 aaacggaaat agctgttttt agcctcataa aaaatattgt gccaactcat aaatattaag
      601 ttatcattaa gtctgtgtaa acgaaccgtt ctaaatttta caagcaactg atttcccagt
      661 aggtttgcgc aaactttcca agacatggaa aacaaagccc gaaaggtgct gatagcagat
      721 gacgaaccgg atattcttga aatcctcaag tataaccttt ccaatgaggg gtatgaagtc
      781 atcactgcca aagacgggga tgaagccctg gaaaaagccc gccgcaccaa cccggacctg
      841 gtgattctcg atgtaatgat gcccaaaaag acaggtgtag aggtttgtca attgctccgt
      901 tcacaggctg cctttaaaga aacgctgatc attttcctga ctgcagtcaa tgatgaaggc
      961 acccagatca agggactgga aaccggtgca gatgattacc tgagcaaacc tattagtcca
     1021 aaagtattcc tgagccgggt gaatgctctg ttccgcagat tgaataaaac agaagccagg
     1081 gtactgacca ttgaaaatat gacgatcgac ccggaacggt ttatcgtaaa agtgggggaa
     1141 gaagaaattg tgctggccaa aaaggaattc gaactccttt acttactcgc ccaaaagccc
     1201 ggccgtgttt tccttcgaaa tgaaatcctg aaccaggttt ggggcaatga tgtgatcgtt
     1261 ggcgaccgta ctattgatgt acatatccgc aagatccgcc agaagctggg tattgattgt
     1321 atcactacgg taaaaggggt cgggtataag tttgaacttt agccccctaa aaggggatgt
     1381 tgtaaaactc ctttgttcac tgctttcact tcgtagctta gcggcatgtt ttctacaaag
     1441 aatatttcac cgagacagct ttctgcgttt acagcattaa tgctggccgt tcctatcagt
     1501 gtgctgattg gttatgtaga aaaaagctgg tggattggtt tggtttgttt tgctattatt
     1561 cttgcaggca gctattttct catctctttc atgctggagc ggttcattta ccgcaagatc
     1621 aaactgatct ataaattcat ttacaaaaca aaagctacca aaagagaaga aacttattat
     1681 aagtatgtgt tgccgcaaaa aagtattgac gaggtaagag aggatgtgga ggcatgggcg
     1741 acaaaaaatg aagaggaact ggaaatgctc cggcagaatg aacagtttcg caaagagttt
     1801 ctgcaaaacc tttcgcatga attcaaaacg acggtatttg ccatacaagg ttatgtagac
     1861 acccttctgt ccggtgccat ggagaaaccg gagatcaata aaaaattcct ggagaagacc
     1921 gccaaaaatg tagaccgtct tactcattta ctcaacgatc ttgatgagat atcaagactg
     1981 gaaagagggg aactggtttt atacaaagaa aattttgtga tacaggacct tgtaaaagat
     2041 gtgtatgaaa gcctttcggt aaagacagag ccactgcata tttactgtac tatcaagaaa
     2101 ggttgtgagt cgcccttaac cgtgtttgct gataaagaaa agatccggca ggtgctgaca
     2161 aacctggtag agaattccat caaatacggc aagcagaatg gcagtattac ggccagtatg
     2221 tacaaaaccg acggcaaaca cattctgatt gaaatcagtg atgatggtat tggtattcct
     2281 gaaaaacatt tactacgcat ttttgaacgt ttttaccgaa cagaagaagg gagaagtatt
     2341 gatgtaagcg gcagcggact tggactggct atctgcaaac acattattga agcccatggg
     2401 caaacgattc atgtacgaag tacagaagca gtaggcacta cggttggttt taccttagat
     2461 tccaggaggg attaaggctg gctaatcaca aaccgataac ctacacctga ttctgtgagc
     2521 aggtattccg gcctgtttga atctgtttca attttttttc gcagttgagc aataaaaacc
     2581 ctgaggtatt gcgactggtt gatgaagcca ggcccccata cagcccgcaa caggtattga
     2641 tgagtgagca ctttgccttc attacgtacc agcaatgcca gtaaactgta ttctgtgatt
     2701 gtgagtttta ctagttcttt attctttttc accgttcttg ttgtaagatc tatctccagg
     2761 tcatgccccc ggatgacagg atcgctatcc tccattacca ctgaacgcaa agctgagcga
     2821 attcttgcca gtaattcgcc ggtgcgaaag ggcttggcca ggtaatcatt ggccccgcta
     2881 tccaatgcag atataatatc atcttcatca tcctgtaccg aaagtattat taccgggttt
     2941 gtaaaccact gcctcaatga ttttaatacc tcctgcccgt ttttatccgg aagaccaata
     3001 tctagcagaa taagatcagg aggatgatta gccgccatta tcaaaccatc ctttgccgta
     3061 gctgcgaggc ttaccgcata accattacta tgcaaggtta tttccagcaa tcttctgatt
     3121 tgcggctcgt catcaatgat cagtatttcc gctttattca tccgctgatt tttttaatgg
     3181 aagatatttc ggcaggtagc ttaatggtaa atatggcacc acctcctgtt ttattctgaa
     3241 cctgcacgga tcctcccatc gcttctgtaa aaccctttac aatggagagt ccaagcccgg
     3301 tgccactaac agttgtgttt ttcaaccggt agaacttatc aaacacaaat ggtatctctt
     3361 cttccggaag tcccttacca gtatcttcta ctgtgaccaa aagatattga tcaaaagaag
     3421 ctcccaatct tatgctggcg tcagccggcg tatggcggac tgcattgctc agcaaattaa
     3481 aaagcacctg ttcaaacata cccttatcca gtttgaataa tggcagctgc tggggaacat
     3541 caattttgat ttgctgtaaa gctccatcct cttccaccct tctcactaca gcgtaaataa
     3601 tttcacttat atcacaccaa ttcattttgg gttgcaaaaa gcctgattca atacgggaca
     3661 tattcagcag gttttccacc tgctgattca gccttaccga agctttggct atttctgcca
     3721 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nncatttacc atagcaatga taaagtacat gatcagcaaa
     3961 atggcgtctt ccgtggaggc aacatgtatg gtgaagtgcg gcggaataaa aaagaaatcc
     4021 cagataaaag cggacagggc tgccgccaga agtaccggaa aaatttcaaa tgagatagca
     4081 atgatggaaa ctgccagcaa caggatcaat gctataaccc tgtaaccaag gtagggcgat
     4141 aatccataac agagcaatga aacagttatc actatcaata cgctgtaaaa atattgccag
     4201 cgtatccccg gtttactttg taaaaaatct ttcattgtct gtaaaagcaa aattagactg
     4261 aagctttcat acacaacgac aagaacagtg tataaagatt ttataaagat ttgatcttct
     4321 gagttggcaa aatcaacgaa gcgacaacag ccacgcatac atttgtccat tcatcatagt
     4381 tcattaccta tgtcaatctc tcacaacaag gcaagtgcag caggtctgct gattgcctta
     4441 ggaatcattt tcggcgatat cggtacttcc ccgctttacg tactcaatgc catcatgaat
     4501 ggaaagacgg tttcagaaga gctgattgcc ggtgccttat cctgtatcat ctggacactt
     4561 accctgcaaa ccaccattaa atatgtgata ctaaccctcc gggcagacaa caaaggcgag
     4621 ggtggtatct tttctctatt tgcccttgta agaagacgaa agaaatggct ggtcattccc
     4681 gctatggccg gtggcgctgc cctgctggcc gatggcatga tcaccccgcc tatctctgtt
     4741 acatccgcta tcgaaggctt gaagcaggtg cctgcccttg ctcatatcag ccaatggact
     4801 gtagtttata ttgttattgg gatacttacc gtactatttt ttattcagca gtttggtact
     4861 gcatttatcg gtcgtttttt tggaccggtc atgcttgtat ggtttgtaat gctggccagc
     4921 ctcggagtag tacaactttt taattacctg ccggtattaa aggcctttaa tccttattat
     4981 gcatataacc tgttaagcaa ttacccgcag gggtttttta tcctgggcgg cgtctttctt
     5041 tgcaccaccg gtgcagaagc tttgtactcc gacctggggc attgcggcaa atggaatatc
     5101 cgttattcct gggtttttgt aaagacctgc cttatcctga attatctggg ccagggggca
     5161 tggttgttac attatcacaa tggtgagatg atcagtgcat ccatgattga gcagggcttt
     5221 aatccttttt atggtattat gccgcactgg ttcatgtatt ttggcatcag catagccact
     5281 gctgccacca ttattgccag ccaggcttta atatcgggat cgtttacact tgtcagcgaa
     5341 gccatgcggc tcaacctgtg gcccaagctg cgtattaatt acccaaccga aacaaaaggg
     5401 caactttata ttcctgcagt caattttttg ttgttcgccg gatgtattgg cattgtactt
     5461 tacttccaga aagcttctaa tatggaagcg gcttacggac tggcgattac tgctactatg
     5521 attgccactt ccgtattatt tgccaattat cttgtactgc accggacaaa gtcagtgttg
     5581 gtttacctgt accttattat ttatttagcc attgagttca gtttctttta tgccaacctt
     5641 gacaagtttc cgcatggtgg ttatgttaca ctaatcgtag gcggattact cttcatcatc
     5701 atgtacgatt ggttcagggc gagaaagatc aagaacaggt atgtggagtt tgtaaggctt
     5761 gagcattatg taccgaaaat tcaggagctg agcaacgata agtcagtgcc caaatatgcc
     5821 acacatcttg tgtacatgac cagtgctgac aatcccaagg agatagagca caagatcatc
     5881 tattccattc ttaaccggaa gcccaaacgg gcagacatct attggctgat acatgttgaa
     5941 acactcgacg atccttacac aacggaatat aaggttaccc atatcatacc aaacgatatt
     6001 atacggattg atttccggtt aggattcagg attcagcccc gtatcaatct tttgttcagg
     6061 aaagtagtag aagaccttgt caccaataaa gaagtaaata ttaccagccg gtatgaaagc
     6121 ctggagaaaa ataatgttac cggagatttt tcctttgtag taatggaaaa atacctgagc
     6181 caggacaatg agcttccttt tattgagcgg ctgatcatga aattnnnnnn nnnnnncatg
     6241 aaattacatt ttgggctgaa ggaaatcagt ctttcagaag aaaaagggtt tggtcttgat
     6301 accagtaatg tggtggtaga aaaatttccg ctgattgtat cgcctgtagc taaaataaaa
     6361 attacccgtc ttggttaata gatgacaagc cgtaaagcca aaatattgtc cttacttatc
     6421 attatacttg cctggctgct ggctttatca cttctatata ttgtttggca aaaggcaagg
     6481 tattttctta gctaatgcat ttttgtaata aggtattagg aatgatcagc gaaataaaag
     6541 cttgcaaatc cgcttgagat tattttatta cagttttccc gatctttagc aaaaggattt
     6601 tattcatggc aaaaagaaaa gattatatct cctgggatga atactttatg ggagtagcgt
     6661 tattatctgc caaaagaagt aaagatcccg gtacacaggt gggtgcctgc atcgtaaacc
     6721 agaagaataa gatcgttggc gcaggttata acggtttacc ggccggttgt gatgatgatg
     6781 aatttccctg ggataaaact ggtgaattcc tgcaaacaaa gtatccctat gtatgccatg
     6841 cagaattaaa tgcaattctg aataatatcg gtatggacct gcatggctgc cggatctaca
     6901 cggcgctgtt tccctgtaat gaatgttcca aagccattat acaggcaggc attaccgaag
     6961 ttatttacct gtcggacaaa tatgccggta atgaaacatc cgttgcttcc aaacttatgc
     7021 tggataaagc taaagtaaaa taccggaagg taagatcatc aatagaaaaa attgaattgt
     7081 catttaatgc aggaaatgtt taattagttt tctatacctg gttgcagaag gaatagcaaa
     7141 gaacaatttg caggactata aagaaaaagt aaaccggcta ccctttccag gttctgagga
     7201 aacaccgata cttcctccct gggcatcaat aaactccttg cagatagcca ggccgaggcc
     7261 tgaaccttct ttattgccgg gtattttaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc acctggtttt ctttttgctg tgccccaata
     7381 attatttcag cattttctgc tgaatgacgg atagcattag taagaaaatt gttgagtacc
     7441 caggtagttt tatctgcatc agccctgatt aaggggaggc tgtcagccac ttcttttcta
     7501 attgtaatgt tcttttgttt ggctgtattc aacacggata gcactgcctt ttctactaat
     7561 tccccaggtt gcacttcccg tatcaagagt tgtatttttc cgctttctac ctgtgacata
     7621 tccagtagct cactaaggat cctgagtaaa cgcttatnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnctgtt cggatgtaag gcttcctatt ctttcatcct ccagcagttt
     7741 taaggtaaag tcagatgagg caagcggtgt tttgagttca tgagaaatgg tggcgataaa
     7801 atttgtttta gctacatcca gttctttaaa cggtgtaatg tttttcagca caattacatg
     7861 gccggttttc tgtccttcgt gagaaagctc cattacctct ttaaaaaaat agttttcctt
     7921 tccatctact actattttaa aaggactgtt actgtgttcg ttaagtaaaa acctaaacag
     7981 atcattacgt ttcattaccg tttctttttc ggcccctacg atatctggtt cattcagatt
     8041 taacaattgt aatgcctgct gattcgcaaa aagtaccgta ccatttttat ccatcccaat
     8101 actggcgtct ttgaaagaat taataactgc ttccgccctt tgcttttcaa aaagaatctt
     8161 tgcaaggcta ctgtgttcat actcatccag tcgttcggcc atgctattga aggcttcggc
     8221 cagtgcaccg aactcatcct tacggttcag gtgtattctt tcactatatt ttttattcgc
     8281 taccgcctta atcccttctg taagtttggc taccggagaa gccaccagtg atgggaaatt
     8341 gaactggaaa gtgaaaccca gtagcaaaca gatggtgata caaacagtaa ctatnnnnnn
     8401 nnnnnngtct ttgctttatc tgcagccacc tgagctgcct ggttttttgt gtcaatcgcc
     8461 ttcaggttca ccttcataat atcattgata ttacttcgta tattaattgc caataaaccg
     8521 gtttgtcctt tgtcttttac cttgttgaaa ttctgcctta aggattcagt caattccctt
     8581 tcaccggttt cagtaatatt attttcctgc aaacggaggt tttgttcaaa cacaacaatg
     8641 gcagatgcgg aatcatcccg actatcaagt acccgaagca tctctcttga ataattcaat
     8701 gactcataat tatcttgtac aatattttta gcatcctggc ttactttatt gaaatagaag
     8761 aagcttacta ctgttaccgt aatcaacagg acgaaaagaa aaagcccccc cagcgctatt
     8821 ttagttttca gtttcatcgc catcaggata aaattatcaa gtccacttcc ctggctgaaa
     8881 gcgttttcaa taactggtta aaaacattag tacggagaat cacctgaaac aaatttaaat
     8941 gaggtttgcc gatgcagacc gttgtaatct ctttttctgt tactgtttcc ataatcgctt
     9001 tagcaatgtt agtgtgttta acctgtaata cttctgcgcc cagttcagtt gctgttttta
     9061 aattgttaat caggtggcgc tgtgccgcca acggaatttt gtcggttgct tctttaggcg
     9121 tttgcacata caaaacatac cactggctgt tgtaatagga agccagcctg ccggtttttc
     9181 ggataatacg ctgtgctatt tcatgattag tggaaatgca ggcgaggaaa tgttcatgcc
     9241 ggaagggtct ttctttcgat gtaacctcat aatctacnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn ngatcatata tttttccttc tttcagcctt gtaatcagtt
     9421 catcggctgt aagatcaata ttcactactt catcggccat ttgcaacagc ttatcaggca
     9481 ctctttcggt tactaccacc ccggtaatat cctttacatc ttcattaatg ctttcaatat
     9541 gctggatatt tacagcagta atcacatcta tacctgcttc taaaatatca attatatcct
     9601 gccagcgttt ttcgttcttg ctgccgggaa tattggtgtg tgccagttcg tctacaatca
     9661 ctacatctgg ctgcaaaaga aaaatagcct gcacatccag ttcatccaat tctttgcctt
     9721 tgtaaaaaac ttttctccgc ggaattatag gtaaaccatc caccagggct tcagtttctt
     9781 ttcttccatg tgtttcaata taaccaacct ttacattcac tccattacgt agcagggcat
     9841 gggcctcctg aagcatacga aagctctttc caacaccggc actcataccg atatagatct
     9901 tcagcttacc tttttgagat tgctgaattt tttctaacca tttatttgct tcactgtccg
     9961 gcattataat gaaactgcta ttgaagaggt gaaaaatgta ttgctttcag tagtgccaga
    10021 tggcttcaaa aatatcttgt ccttactgct gaggtttctt atttcaagcc gtaccactac
    10081 gtttttaaag gggctgtggt caatattcag cgaaaaaccg gttgtctgga aaccatttgg
    10141 gcttgctgtt gtgataatga cttcgttctt gtcattgtaa tgctccaccc ttcctgcaat
    10201 agccaacttc ccgttgaagg catagcgaag tatcagcgct ggactgtacc agatgttttt
    10261 atcactgccg cctggagttt tttcttcgga acctatatca acattggcga tgatgccaaa
    10321 tttatcagtg acattgaaaa tcccgtaaat attatgaaag aatcttgaca gccgggtact
    10381 atctggtttg tcagtgccaa taaatgtgcc gtagtttagc aatacttttg acgaaggctt
    10441 gaactgaatt tgtgtgcccc agctcatcaa tgagttacca cgnnnnnnnn nnnnnnnnnn
    10501 cttgttatac gctgccagcc gtttaacgcc atcccgctga agaaccattt gccgtcatca
    10561 gttgtgtagc tgattttggc gccggcttca aaatagggag tgttatcagc cagtatagaa
    10621 cgggtaaggt tccagcaatc tttactcacc gcactttcaa aaccaatatg tgaaggcatg
    10681 atacccgcat ctatccacaa gtttttcttt ttactgatct taatactggc attggcttca
    10741 aacacatttt tcaggacccc tggttcggca gcataattgg cattcatata tgtgcctgct
    10801 gctaatgcaa tatttgccct gactctttct gcagtataat ttaccctgag aaatcccaga
    10861 ttcaggttaa actcgttgtg gcggttatgg ctgtaaataa agcctggcct gttattatca
    10921 gccggtttat taaaatcgta tgtgtaataa gcttctgcat aaccactaag cgtcaggttt
    10981 acggttgagt cctgtgctgc aagggatata ttcattaata cagcagttcc aatcaaaatc
    11041 tttttcatct ttttattagt atgttatttt aaattatcta aggcaatatt cagtttcaat
    11101 acatttacta cggaaggacc aagaatcgtt ttacttattt gctggtctac caaagttctg
    11161 actttatctt cggcaagttt acggatggca gctactcttt tcacctgcac ataggcggac
    11221 gccggggtta tatccggatc gaggccactg ccgcttgccg taacaaggtc agcaggtatc
    11281 tcttcttttc ttacccctgg attatgaacc aaaaaactgt ctattctgtc ctgtactatt
    11341 tgcaaataat caggattgga tgggccttta ttagttccgc cagaaccggc cgcattatat
    11401 tccactgcag aaggccggcc ccagaaatat ttatcttctg taaatttttg tccgacattt
    11461 tcgtagccca ccactttacc atttacagaa accgttctgc cttttccgcc gccaggggtg
    11521 gctttgccaa tacctgctat tagtaaagga taaatcacag cacatagtat aatcatcaac
    11581 aatgtgagtt tcagcgatat taaaagactc tttttcattt ttattgtttt acataaataa
    11641 cccgagtaac atatctataa gtttaattcc tataaatgga acaatgattc cgcccaggcc
    11701 atagatgaat aaatttcttc tgagcaatgc cgatgcaccg attggtttat actgcacgcc
    11761 tttcagcgcc agcggtatca atgcaggtat aatcagcgca ttgaaaatga tagcagaaag
    11821 gatggctgac tcaggacttt tcaatcccat aatgtttaga gattgcaaag caggaatgga
    11881 tgcgatgaat aaagctggta caatagcaaa gtacttagcc acatcattgg caatagagaa
    11941 agttgtcagc gttccccttg ttatcagtaa ttgcttccct atttccacta cttcaatcaa
    12001 tttagtgggg tcattgtcca gatccaccat attgcccgct tctttagctg cctgggtgcc
    12061 gctgttcatg cttaccccaa catctgcctg tgccagcgca ggcgcatcgt tggtgccgtc
    12121 gcccatcatg gctactaatt ttccgccttg ttgttcctta cggatataat tcatcttgtc
    12181 ttccggctta gcctcagcaa tgaaatcatc cacaccggct ttttctgcta tgtattttgc
    12241 agtaagcggg ttgtcgccgg ttaccatgac agtctttact cccattttcc gtaatctttc
    12301 aaaacgctct tgaataccgg gcttgatgat atcctgcagt tcaattacac caatcacttt
    12361 ttcattcagg ctaaccacca atggtgtgcc gccattggaa gaaattagtt tcactctttc
    12421 tgctgtttct ttaggaaatg agttgccttc ttttattaca atattttgta tagaatcaaa
    12481 agctccttta cggatccttc ttccgtcggg caggtttacg ccgctgcttc ttgtttctgc
    12541 ggtgaaatca ataaatgtgg caccttgtgt attgtagctt ttagtattta tgcccgccag
    12601 ttcaataatg gattttcctt ccggtgtctg atcggataaa gatgataaaa cacaggcttc
    12661 aataaaattt tttttgtcaa ttccatttgc gggataaaaa ttggtggctt tcctgttgcc
    12721 aatagtaatg gtacctgttt tgtcaagcag caacacatct acatcgccgg ctgcttctac
    12781 agctttacca cttttagcga taacattagc tcttaaagcc ctgtccattc cggcaatacc
    12841 aattgcagat aacaaaccgc cgatagtagt cggtatcagg caaacaaaca gtgaaatgaa
    12901 tgctgcaata gtgatgggtg tattggcata atccgcaaaa ggtttcagcg ttacacatac
    12961 tattataaat atcagggtga atgctgccag taaaatagtc aatgcaattt cgttgggtgc
    13021 tttttgcctg gaggcacctt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgccaccgg
    13141 taacagaact tttatctccg cctgcttcac ggataaccgg tgctgattct cctgtaatgg
    13201 ccgattcatc aatggtagca ataccttcca ctatctctcc atccatgggg atgatatctc
    13261 cgggttcgca gacaaaaaaa tcacctttgc ggagctgtga agaagaaacc acttttattt
    13321 cattcacata catctcacca acggtcagca cttttttagc aggtgtctct tccctggtgc
    13381 gacgaaggct ttcggcctgt gcctttcccc ttgcttcagc aatagcttcg gcgaagttgg
    13441 caaacaatac cgtgagaagc aatatggcaa agactgtaat attataccac aaagcacctt
    13501 cactggtatt gtcagttatt agttgccagc tgcaaacggt cagcataata gcggttccaa
    13561 tctccacggt aaacattacc ggattcttaa ccatcagcct tgggctcagt tttataaatg
    13621 actgcctgaa ggcagtgccc acaagggccg gttccaataa tcttacagta cgtttatgct
    13681 tagtcatttt atattcatta atacagtgaa aaatattcag ctagtggacc taatgccagt
    13741 gcaggcagat aggacagagc agttactatt ataattactg ccaatgtcat agcgccaaaa
    13801 gtgtaagaat ctgttcttaa tgtaccgggt gattcaggga tgtatttctt ttttgccaat
    13861 aaaccaatga tagcaattgg cgctataatc ggaagaaacc tgcccagtaa taaagcgatt
    13921 ccagtagtta cgttccagaa aatattatta tctccaagac cttcaaagcc actaccattg
    13981 tttgcattgg ctgaagtgta ctcatataac atttctgaaa aaccatgata cgaaggattg
    14041 ttcagccagg cacccggttt tacagcccat cccatttccg gatgatgcgc caccaggtat
    14101 gctgcaaagg cagtgaaccc ttttattaaa aatgcagaga gcaatgtaac cagcgctgca
    14161 atctttacct cccttgcttc tactttgtga cccataaact ccggcgttct tcccaccatc
    14221 aaaccggaaa taaaaacagc gataatgata aatatgaaat aattcagcag acccacacct
    14281 acccctccat ataaggcatt tatcatcatg tccagtatca ctacgccgcc actgatgggt
    14341 gttaagctgt catgcatacc attaacagaa ccattggaag tagaagtagt ggcaacactc
    14401 cacagtgcag tagctgctgc acccagtctt acttcctttc cctccatact gccggtggtt
    14461 tgggcaatgc ccagctgatc aatagccgga ttgcctttag tttcataata gatattgata
    14521 gcacagaaga tgacaaataa aaatgtcatt actgcaaaga tcacatttga taattttatt
    14581 cttttgagat aataacccag tgcaaataca agtgcaatag gaataattaa tatagaaata
    14641 gtttcaactg cattggtgat atagtttgga ttttccaacg ggtgtgccga gttgacacca
    14701 aaccagccac caccattggt gcccaattgt ttgatggcaa tcattccagc tgccggacct
    14761 cttgatactt gtacagtatc tccctgcagg gtgatgatag tatcttttcc atcataagtt
    14821 gtcggcgtac cattaaaggc caggatcact gctacgagaa tggataaggg taaaagtatc
    14881 cttgtaatgc tttttacaaa gtagttccag aaattaccca ggttattagt ggtcttttct
    14941 tttataccat tgaataaggc caccagcatg gccataccgg tcgctgcgct gacaaattgc
    15001 agaaaagtaa taacaaacag ctgggtgaaa taagttaaac ctgactcacc tgaatagtgc
    15061 tgcaggttac agttcactag gaagctgatt gctgtattaa aagcaagatc cggtgtttgt
    15121 ccagggttac catccggatt tagtggcaat ttgtcctgga aaatcagcag gagcatagca
    15181 tagaccagcc ataacatatt aatggtgagc atagctttca gaaactgttt ccagttcatg
    15241 ctttcagcgg agttaatccc acaaaaccgg aaaaaaaatt tctcaaccgg tttcataaag
    15301 tcgctaatgt tcttatttcc ggcataaact gcggaaatat atttgcccag cggccatgcc
    15361 agcaatatgg ttgccccaaa aatgataata gtgccaagta tttcgctgtt catggtctca
    15421 gaatttttca ggttttacca atacatacag caggtaaaaa aacactgcaa tggaaaggat
    15481 aaataatatt gtcatcatgt tgttcaaata ttttcaaacc aatcaatgga tttataaaaa
    15541 agccagaata aaatcagtcc ggcggccaga agtataacag taagcatgtc tacagatatt
    15601 ttattaataa agcaataatc aggctgccga aatacaagac tgcaataagg acatgcagcc
    15661 tcaaccggta accgattctc tttttggtag aattcatttt ctttctttaa aagggaaatg
    15721 ccaaacagag tgcctgcaac ttgaagagaa aggaggagta aagcaaaaag cctggttaac
    15781 aaagctttaa atgaaaaaag taattttctc aaacaagcct gaaaaatta
//
LOCUS       DAORLS010000033        14994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2132_length_14994_cov_2.162728, whole genome shotgun sequence.
ACCESSION   DAORLS010000033 DAORLS010000000
VERSION     DAORLS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2132_length_14994_cov_2.162728"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>228
                     /locus_tag="PK977_04290"
     CDS             <1..>228
                     /locus_tag="PK977_04290"
                     /inference="COORDINATES: protein motif:HMM:NF021922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HRF17358.1"
                     /translation="DFPGGWQGDKANAFTGVGLSADQQRIQAYLKKLLNWRKDKKVIH
                     DGSTLHFAPSNGIYVYFRYSKQEKVMVVMNKN"
     assembly_gap    229..353
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            443..1576
                     /locus_tag="PK977_04295"
     CDS             443..1576
                     /locus_tag="PK977_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF17359.1"
                     /translation="MNYRVLLVSFLLIITSSSFAQTAKELYEEGVQLRKDGKSKEAIE
                     RFKRAVALKPDYIEAYYEMGWCQNETKDYTGAIASLQKARQEWSAVAKVHFELGYAFE
                     KTSQNDSAMASYKRCLAINNEYSLAWKQLGYMAYLKDDAKTALEHFAKYEAAAKNPIT
                     DYLYWYRKGFCFNGVKDFNNAKTALFKSLEYKKDYVNTYLELGFACSRLKEDDAAIGY
                     YKNAIEIDSNSHIPYNGIGEVYRDNKKNFSEAMVWYQKTLALNPNERKGNYGMGYCLN
                     ATGKHTEAIDYLKKAIQNEKDYVAAFVEIGYSYYKIGYFKEALANLTKAISLNPKNEN
                     GRYYACLVYISLGEKSNAQRMVDELKSLSSRHAAGLQERVDKM"
     gene            complement(1723..2013)
                     /locus_tag="PK977_04300"
     CDS             complement(1723..2013)
                     /locus_tag="PK977_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HRF17360.1"
                     /translation="MVQKTYYKTKDYCKVKFSFKVENAETIEILGLNSDWENSVIMSR
                     KKDGSFSADVNLPKDSTHEFKYLVDQKEWLNEPEADKEVPNVYGGSNSVITL"
     gene            complement(2196..3353)
                     /locus_tag="PK977_04305"
     CDS             complement(2196..3353)
                     /locus_tag="PK977_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HRF17361.1"
                     /translation="MSTISNELFYNRLPVNQLSLGELLTEEHLFYRIPANWHVLLTDV
                     KKSTEAVTQGRHETVNMVATASIVAVLNIAYKQNISVPFFFGGDGATFLVPPVILEPA
                     LKALLQHQENTNANFDLQLRVGHVAVEDIYKMGCQLLISKLKTSDLFSIPVVLGNGLA
                     HAEKIIKGDDYLLTPLSADEALLDMSGFECSWDRIKPPEQYDEVVSLLVLAKDASLQA
                     SVFKQVADTIDAVYGEPEKRKPITESQLKLKRTLQKISHEMRGKFGRFNLLYLTGTWL
                     KLLLGPLYFKTRAGKSYLNQLVQLSDTLVIDGKISTVISGTAAQRTELEKELDRLETE
                     GAILYGLYVSRESVLSCYVRSRNEKHVHFVDGADGGYTRAAMVLKQKLAKP"
     assembly_gap    3434..3446
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3531..3782
                     /locus_tag="PK977_04310"
     CDS             3531..3782
                     /locus_tag="PK977_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17362.1"
                     /translation="MTQPFYNDQAYIAEGFHLDEDFTEQTARLAFFKINSYKLSLLKS
                     SFRQTRQELKQSLKETLFNYTDPAIILADTGFFGGEYEN"
     gene            4129..4380
                     /locus_tag="PK977_04315"
     CDS             4129..4380
                     /locus_tag="PK977_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17363.1"
                     /translation="MKLSEFIALSEEEKRAFVLQQGVAVAKRQLPGYLVFLYQLSYFY
                     VETYCCQQTKEILEYRTIYSTKHLKPYLESIPIDHLLNT"
     assembly_gap    4499..4534
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4714..>7166
                     /gene="smc"
                     /locus_tag="PK977_04320"
     CDS             4714..>7166
                     /gene="smc"
                     /locus_tag="PK977_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRF17364.1"
                     /translation="MRLKSLEIKGFKSFADKTIVNFDDNITGIVGPNGCGKSNIVDSI
                     RWVIGEQKISHLRSENLDSLVFNGSKTRSASGLAEVSLTFENTKNLLPTEFSTVTITR
                     KFYKSGESEYRLNDVQCRLKDIQNLFMDTGVSTDSYAIIALDMVDDLIKDKDNSRRRM
                     LEQAAGISIYKTRKKEAKQKLEATEQDLARIEDLLFEINNQLKALESQAKKAEKYHEI
                     KKEYREVSIELAKAALEGFNLSYKELNEQQEIETDKKVKLEAEIATDEAAVEQEKLAF
                     VEKERLLQAMQHAFNEMVQQVRTKENEKNLSAQRLEHLKEKESSLSGFLQKAEGQLKG
                     IDESIGFTNLQVEEEEKALAGLNEQLEQLRQESDAKRTVLDEKRRHLDELRSEYQHIQ
                     RNQFDAEKKVAVADSSVQNLQRIIGQIEEERKHREDQRHHLDQERILKEKELSIKKVD
                     LEQLQQHQENTKEQILQTQSVLDSLRNNLAEETRKLDAKKNEHDLLKSMIDSMEGYPD
                     SVKFLHNNSNWSHNSPILSDVLYVKEEYRTALENVLEPYLSYYVVNNLQEGLQAVHLL
                     DENKKGKANFFLLDKVNDGASGVSAHQLEGAIPALSVVEVDEKYSKLAEHLLGNVFIA
                     DNEDALQNSNGFVVIEKHGKYVKGKYSLTGGSVGLIEGKKIGRVKNLEKLEEEIAAQD
                     TVVEALRSEIQARHNEVIAFNEDLRENAVRETEREIQQLTNEVFSLHNKLENLHSLQT
                     NSQHRLEELNLQVQQALSSVEGVRNQLGEYNELLQANAAKLAEADEAFKITEAAYNQA
                     TQQYNDFNLQVTKQQSKIAA"
     assembly_gap    7167..7225
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7226..>8126
                     /locus_tag="PK977_04325"
     CDS             <7226..>8126
                     /locus_tag="PK977_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRF17365.1"
                     /translation="DLRQQIENNTAQLAETSSNIEQSSQSLKDIEEALVQLMKSKEEE
                     ELKLNEADQAYYNFRNQLSEKESEIRHKVKDKEQVEHMLAAIKDKLNELKLQLAGMKE
                     RMNVEFRIDLESILGEARTGEVPVEELQEKADRMKKRLENLGEVNPTAIEAFTEMKKR
                     YDFIVEQKTDLVNAKDSLLKTIEEVEAKANQKFLDTFHLVRDNFQKVFKTLFTEDDQA
                     DLVLENPENIAETGIDVVAKPKGKRPSSLTQLSGGERTLTATALLFAIYLIKPAPFCI
                     LDEVDAPLDDANVGKFTNMIRQFS"
     assembly_gap    8127..8160
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8346..8684
                     /locus_tag="PK977_04330"
     CDS             8346..8684
                     /locus_tag="PK977_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRF17366.1"
                     /translation="MRTIILLFASNVFMTFAWYYHLKHQGWPLWKAILISWAIALFEY
                     CLMVPANRIGKVDGFNLFQLKMIQEAITLIVFCAFAVWIMKEPFQWRYIVSFVLLMGA
                     VYFMFSKQPV"
     gene            complement(8689..8955)
                     /locus_tag="PK977_04335"
     CDS             complement(8689..8955)
                     /locus_tag="PK977_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17367.1"
                     /translation="MPNSHSDKYVTNLLLGFSFVTGSIFLLLFPIFEKEQKDDWYIWA
                     IVASLILCIGLYFLVSAAVHKVKSDLIKRQRTREQLQHRHEKRA"
     gene            9099..9683
                     /locus_tag="PK977_04340"
     CDS             9099..9683
                     /locus_tag="PK977_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HRF17368.1"
                     /translation="MKKIIAAFLSVFMLHIVSAQEAPEGLFIGSKAPDFKAKDQNGNE
                     VRLKDLLKKGKVVLVFYRGQWCPYCNRELSRIQDSLQLIKDKGATVVAVSPEKQESIA
                     ATVEKTKAEYPVLYDEGLKIMKAYDVEFEVPENTIIRYRNANIDIEKNNGSNGKYLPV
                     PAVYIIDKESTVTYRFFDTDYKKRPWVKDLLNNL"
     gene            complement(9687..>10154)
                     /locus_tag="PK977_04345"
     CDS             complement(9687..>10154)
                     /locus_tag="PK977_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRF17369.1"
                     /translation="DRDEAVFVSGGVKSRLFIRDKTTPLPIIQGQETTGANSIAVWDK
                     LKMKGGKRMIVVGGDFKADSSRSQNCCFTNDGGQTWETPHTPPHGYRSCVEYFSKTDV
                     FACGLNGVDYSNDGGKNWKWISKESFHVVRASKLGAAIYFAGEKGKIGKIVWR"
     assembly_gap    10155..10185
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10186..10726)
                     /locus_tag="PK977_04350"
     CDS             complement(<10186..10726)
                     /locus_tag="PK977_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRF17370.1"
                     /translation="MTSKNILCFVLVTAMQLYKVSLFAQGAAVEVLTSGTNTSIRGLS
                     VVNDNVIWVSGSKGTVGRSSNGGKTWKWMIVKGFENKEFRDIEAFDASTAIIMAVSEP
                     AYILKTIDGGESWKIVYENKDKGMFLDAMEFWNEKAGIVIGDPINGRFFISRTFDGGN
                     SWQDIPFDKRPVADSGEACF"
     gene            complement(10744..11670)
                     /locus_tag="PK977_04355"
     CDS             complement(10744..11670)
                     /locus_tag="PK977_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF17371.1"
                     /translation="MKRKAFLQNTAAVIGGSMLPSFARAAEEKRKWIRFAHLSDIHVK
                     PGLLPEAGMARALQHVQQLNRKVDFIVNGGDAIMDALEADKSKTQTQWDLFKSILQKE
                     NSLSVYHTIGNHDVWGWFIKSNKPDNDRLYGKVWVVETLEMPKRFYSFTKANWKFIVL
                     DSTQLNPAGGYIGKLDEEQLIWLKEELKNTASTQFICIISHIPILSICAGLFFNKTEA
                     NGDLMIKRNLMHSDFLVLKPIFRQYPNIKVCISGHIHLQDEVDYLGIKYYCNGAVSGN
                     WWNGSFQEFAPAYALMELGPEGQVSRTMTPYQ"
     gene            complement(11673..12365)
                     /locus_tag="PK977_04360"
     CDS             complement(11673..12365)
                     /locus_tag="PK977_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF953 domain-containing protein"
                     /protein_id="HRF17372.1"
                     /translation="MKNVLVIALLFTFSGCQSQSGPNSKVNAEEFEKGISAPNVQILD
                     VRTAAEFSSGHIKNALQANWVNPPEFTERVKYIDKDKPVYVYCLVGSRSTGAANWLRE
                     NGYKKVVELQGGINAWKRANKPVEGGGNQQQMSIADYQAMIPADKTILVDFGASWCPP
                     CVKMNPVLDDLQKDASLQFQLIKVDAGIHTDVMKALNIEPIPVFVIYKNGKEVWRKQG
                     IVSKEEFIAQLK"
     gene            12516..>13488
                     /locus_tag="PK977_04365"
     CDS             12516..>13488
                     /locus_tag="PK977_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17373.1"
                     /translation="MKKQLLYLLAIGLVVIIGCQKELSFEGDSTPAEGSLQSDINGDC
                     LPKSVNGVYVAAKPLAADTNTISVSVNVVKTGSYVISSDTVNGYFFRATGIFTTPGVN
                     TVTLRGNGTPFAAGVNNFVITFDSTVCDIQVTVLPAGAGGPATFTLVNGGTPPNCASA
                     VVNGTYTKDVALTAANTVDVTVNVTAIGTYNISATGGGMTFTGTGAFITLGNQTVTLT
                     GSGTPTTTGANTVTFAAPFASCNFTVNVTGPCEGTLGGGPGACTPSIVSGIYVVGSSL
                     SGPGNNVQIQVSITTAGAYNISTNTVAGITFSGTGTIAVGPNQNINLV"
     assembly_gap    13489..13571
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13572..14198
                     /locus_tag="PK977_04370"
     CDS             <13572..14198
                     /locus_tag="PK977_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17374.1"
                     /translation="NVLPNDYFPRTTNSNWSYEWDNDPTDSLYRFAISATNATAFGTF
                     NIFMENDGTAPPAGDSSGYYRRSGGDYFEYFDLSAIGLDNPQWVEYTFLKDNVAAGNS
                     WTSTGWTNALGGTPFTFRFKDSIEQKDVAVPQTTSLGTVNYQNVIVVVERIQLQVGPA
                     WVDASSVFGYAKYYYARGIGLIRIELFDPPGTSPTPSFVQRLRRHQVF"
     gene            complement(14259..>14994)
                     /locus_tag="PK977_04375"
     CDS             complement(14259..>14994)
                     /locus_tag="PK977_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRF17375.1"
                     /translation="AINPNMSELEIQGLHEYVHKKYGAESVGYGSIIGAGENGCILHY
                     MENSKTKVGTSMLLMDVGAEYHGYTADVTRTIPVDGKFSNEERQIYQLVYDAQEAAFK
                     TLKDGSKWADASAAAQQVIAEGLLNLGIIKNKNEVSKYYPHGLGHHIGMDVHDRGTYD
                     KLKKDMVITIEPGIYIPEGSNCDKKWWSIAVRIEDDLLITETGYELLSHFAPRKIEDI
                     EKKIVEKSAVDDFKLPPLESGKKKGF"
BASE COUNT         4178 a         3150 c         3306 g         3979 t
ORIGIN      
        1 gattttcccg gcggctggca aggagataag gccaatgctt ttaccggcgt cggtttatca
       61 gcagatcagc aacgcataca ggcatacctg aaaaaactac tgaactggag aaaagataaa
      121 aaagtgatac acgatggaag tactttacac tttgcaccat ccaacggcat ttatgtgtac
      181 ttccggtaca gcaagcagga aaaagtgatg gtggtcatga ataagaatnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcaacgg
      361 tatgggagat caggtagtct aataccctgc agacggtata ggcttttatc tttctgcttt
      421 tagtttcgct aaaaatcgaa ttatgaacta tagagtgctt ttggtatctt ttttactgat
      481 cattacgtcc tcctcttttg cgcagaccgc aaaagaactc tacgaagaag gagtgcaatt
      541 gcgcaaagac ggcaaatcca aagaagctat tgaacggttt aaaagggctg ttgcgctcaa
      601 acctgattac atagaagcct attatgaaat gggctggtgc cagaatgaaa ccaaagatta
      661 caccggggcc attgcttcac ttcaaaaagc cagacaggag tggagcgctg tagccaaagt
      721 gcattttgaa ctaggctatg cttttgaaaa aacttcgcag aacgattcgg caatggcatc
      781 ctacaaaaga tgcctagcca ttaataatga atattcgctg gcatggaagc aactgggcta
      841 tatggcctac ctgaaagatg atgcaaaaac agcgttggaa cattttgcca agtatgaagc
      901 tgctgcaaaa aatccgataa ctgattattt gtactggtac cgcaagggtt tttgttttaa
      961 tggtgtcaaa gattttaata atgccaagac agccttattc aaatctctgg agtataaaaa
     1021 agattacgtc aacacttatc tcgaactggg ctttgcttgt tcccggctta aggaagatga
     1081 tgctgcaatc gggtattaca aaaacgccat agaaatagat tccaacagcc atattcctta
     1141 caacggtatt ggtgaagtgt accgtgacaa taagaagaac ttttcagaag cgatggtatg
     1201 gtatcaaaag actttagcgc tgaatcccaa cgaacgcaaa ggaaattacg gtatgggcta
     1261 ttgccttaac gctaccggta aacataccga agcgatagac tacctgaaaa aggccattca
     1321 gaacgaaaag gattatgtgg ctgcttttgt agagattggt tacagttatt acaagatcgg
     1381 ttattttaaa gaagctcttg ctaatctgac taaagccatc agtctgaacc ctaagaatga
     1441 gaatggccgc tactatgcct gcctcgtgta tataagcctg ggcgaaaaaa gcaatgccca
     1501 gcggatggtg gatgaattga aatcattatc atccaggcat gccgccgggt tgcaggagag
     1561 agttgataag atgtagtgtt ttttctgtca tgctgagcct gtcgaagcat agacagaaaa
     1621 acgaaacctt gattttcatt aatcatcctt cgacaagctc agggtgacaa aggagaacat
     1681 agccactccc aacactgatc ttaataatat atctaagctg aattacaggg taatcacact
     1741 gttgctgccg ccatacacat tcggtacttc tttatcagct tccggctcgt tcagccattc
     1801 tttttggtct accaggtatt tgaactcatg ggtgctgtct ttcggcaggt ttacatcagc
     1861 gctgaagctg ccgtctttct tccggctcat gatcactgaa ttctcccagt cgctgttcag
     1921 gccaagtatt tcaatggtct cggcgttttc taccttaaaa gagaatttta ctttgcaata
     1981 gtccttcgtt ttgtaataag tcttctgtac cataatcgtt gttataggtt aacaattttt
     2041 attcactatt tcatacgggg aagtcaatcg tcaggagatt tcctggggcg gcaattgccc
     2101 gttaatacgc aaatgtaaag aaagtaacct gccggctttg cagcagttat tgacgggctg
     2161 gggaaaatga aaattgggta ctggctttca acgtattatg gctttgccag tttttgtttc
     2221 agcaccatgg cggctcgggt atagcctcca tcagcgccgt ctacaaagtg tacatgtttc
     2281 tcattccggc tgcgtacata gcaggaaagc accgattccc ggctcacata caggccgtac
     2341 agaatagccc cttcggtttc cagcctgtcc agttcttttt ccagttcagt tctttgcgct
     2401 gccgtgcccg atatcaccgt actgatcttt ccatctatta ccagtgtgtc agataactgc
     2461 accagttggt tcaggtagct tttgccggcc cttgttttaa aatacagcgg ccccagcagc
     2521 agttttagcc aggtaccggt taggtaaagc aggttgaagc ggccaaactt tccccgcatt
     2581 tcatggctga tcttttgtaa cgttcttttc agcttcagtt gcgattcggt gataggtttt
     2641 cttttttccg gttcgccgta tacggcatca atggtatcgg ccacctgttt aaacacggag
     2701 gcttgtagcg atgcatcctt cgccagcacc agcaggctca ccacttcatc atactgttcc
     2761 ggaggtttga tgcggtccca gctgcattca aagccgctca tgtccagtaa tgcttcatca
     2821 gccgaaagag gcgtgagcag gtaatcatcg cccttgatga tcttttcggc atgtgccagc
     2881 ccgttgccaa gcaccaccgg tatagaaaac aggtcgctgg tctttagttt actgatcagc
     2941 aactggcagc ccattttgta aatatcctcc acggccacat ggcctacccg caattgcagg
     3001 tcaaaattgg catttgtatt ttcctggtgc tgcagcagtg ctttcagtgc cggttctaat
     3061 atcacagggg gaaccaaaaa cgtagcgccg tcgcccccga agaaaaaagg aacgcttatg
     3121 ttttgcttgt aagcgatatt cagcactgcc actatactgg ccgtggctac catgttcacc
     3181 gtttcatgcc ggccctgtgt tacggcctca gttgatttct ttacatccgt aagcagtaca
     3241 tgccagttgg ccggtatgcg gtaaaataag tgttcctccg taagcagttc accgaggctg
     3301 agctggttta cgggcaggcg gttataaaaa agttcgttgc tgattgtact cataaacagc
     3361 aggtaatgat agggtaagtt agtaatttct tccggcagca gaggagtggc agccgcagcc
     3421 cgcaattttc cgcnnnnnnn nnnnnnttcc gcttagcttt ccgtttaata aaatatccat
     3481 cccggtaatc ctgcaaagga cgggacctat ataaaaccaa tatccgtcgt atgacacagc
     3541 cattctacaa cgaccaggca tatattgccg agggtttcca tttagatgag gattttacag
     3601 agcaaactgc ccgcctcgca tttttcaaga ttaattctta caagttgtca ctgctcaaat
     3661 cttcgtttcg ccaaacccgg caggagttaa agcaaagcct taaggaaact ttgttcaatt
     3721 acactgaccc ggccatcatc ctagccgata cgggattttt tggcggggag tatgagaatt
     3781 aagaaaaagc tataatgcta tttccggcta tagagagttt taataaaaaa aattgtagcc
     3841 actgttatga tgtaagccta agagtctaac agggcttaat actgctttta tatttgtatg
     3901 aaaaaaattg aactaactac tgcataaaat aatttgatac gtccacaatt gtcctaactt
     3961 tataacctgc atgttattta tagcggacag tttttctgtt cggtctcgtt tttgtttatt
     4021 gcctcagcct ctaaagttca ttgattggtg taagtgtcag ggctgcgtct ctgccaacca
     4081 cagttttagt aattgcattt tttacctaac agtttaaaac cggcatttat gaaactatca
     4141 gaatttattg cgttgtccga ggaagaaaaa agagcttttg ttttacaaca gggtgttgcg
     4201 gttgcaaaac ggcaattacc tggttacctg gtgttccttt accaactctc ttatttttat
     4261 gttgaaacgt attgttgcca gcagaccaaa gagatcctgg agtacaggac catttacagt
     4321 acaaagcatt taaagccgta cctggaatct atccctatag accatttact gaatacttga
     4381 tatcgtccgg cttattcctc ttgagtgaga aattgagtaa caagaaatat tccttgcttt
     4441 gggtatttct ttgtgattta gcatcggttc ctgtttcaaa gacctgtttg ctaatttcnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggaaga cgcctgccgg tcggatgcag
     4561 aaaatactaa ccataacaag cagccacaac caaaaacctg ccctgtaatt tctccccaat
     4621 ttacccacca cgctgtgaat aactgcctgt cagccaacgg caattttctt cacctttgcc
     4681 ttatattcgc cgcctgactt aaaactgaaa acagtgagat taaagagctt agaaatcaaa
     4741 ggattcaaaa gttttgcaga taaaaccatc gtcaatttcg acgacaatat aacaggcatc
     4801 gtagggccca acggttgtgg aaagtcaaac atagttgaca gcatccgctg ggtgatcggt
     4861 gaacaaaaga tcagccacct gcggagtgag aacctggaca gtcttgtctt taatggttct
     4921 aaaacccgca gtgcttccgg ccttgccgaa gtaagcctga catttgagaa caccaagaac
     4981 ctgctgccta ccgagttcag caccgtgacc attacccgca aattctacaa aagcggcgaa
     5041 agtgaatacc gcctgaatga tgtgcagtgc cgcctgaagg atatccaaaa cctgtttatg
     5101 gataccggtg taagtacaga cagctatgcc atcatcgccc tcgacatggt ggacgatctc
     5161 atcaaggata aagacaacag ccgccgccgt atgctggagc aggctgccgg aatttctatt
     5221 tataaaaccc gtaagaaaga agcgaagcaa aaactcgaag ccacagagca ggacctggcc
     5281 cgtatcgaag acctgctgtt tgagatcaat aaccaattaa aagctttaga gagtcaggca
     5341 aagaaggcag agaaatacca cgagataaag aaagaatacc gggaagtaag cattgaactg
     5401 gccaaagcgg cattggaagg tttcaatctt tcgtacaaag aactgaacga acagcaggag
     5461 atagaaacag ataagaaagt aaaactggaa gctgaaatag ctacggatga agcagcagtg
     5521 gagcaggaaa agctggcctt tgtagaaaag gaaagactac tgcaggccat gcagcatgct
     5581 ttcaatgaaa tggtgcagca ggtaagaaca aaagaaaatg aaaagaatct ttccgcacag
     5641 agattagaac acctgaagga aaaagaaagc agcctcagcg gctttttaca aaaagcagaa
     5701 ggccagttga agggtattga tgaaagcatt ggttttacca acctgcaggt agaggaagaa
     5761 gagaaggccc tggcaggact gaacgaacag ttagaacaat tgcgccagga gtcagatgcc
     5821 aaacgtactg tgctggatga aaaacgtcgt cacctggatg aactgcgcag cgaataccag
     5881 catatacagc gcaaccagtt tgatgcagaa aagaaagtgg ccgtggctga tagttctgta
     5941 caaaacctgc agcgcattat tggccagata gaagaagaga gaaagcacag ggaagatcag
     6001 cgtcaccacc tggaccagga aaggatactg aaggaaaaag aactcagtat taaaaaagta
     6061 gacctggaac aattgcagca gcaccaggaa aacactaaag aacaaatatt gcagacacaa
     6121 tctgtactgg atagcctgcg caataacctg gcggaagaaa caagaaagct ggatgccaaa
     6181 aagaatgaac atgatctgct gaaaagcatg atcgattcga tggagggtta tcctgattcg
     6241 gtgaagtttc tccataacaa cagcaactgg agccataact cgccaatact ttcagatgta
     6301 ttgtatgtaa aagaagaata cagaacagca ttggagaatg tgctggaacc ttacctcagc
     6361 tattatgtag taaacaacct acaggaaggt ttacaagccg tacacctgct ggatgagaat
     6421 aaaaaaggaa aagcgaactt ctttttattg gacaaagtaa atgacggcgc ttctggtgta
     6481 tcggctcatc agttggaagg agcaataccg gcgttaagtg tggtggaagt agatgagaaa
     6541 tacagtaaac tggcagagca cctgctgggc aatgttttta ttgccgacaa tgaggatgcg
     6601 ctgcaaaaca gcaatggttt tgtagtgata gagaagcacg gcaagtatgt aaaaggaaaa
     6661 tactcactta ctggtggcag tgtcggtttg atagaaggaa agaagatagg ccgggtaaag
     6721 aacctggaaa aactggaaga agagatcgct gcacaggata ccgtagtaga agccctgcgt
     6781 tcagaaatac aggctaggca taatgaagtg attgccttca acgaagatct tcgggaaaat
     6841 gccgtaagag aaacagaaag agagatacag caactgacca atgaggtatt ctcgctgcat
     6901 aataaattag aaaacctgca ctccctgcaa accaacagcc agcaccggct ggaagaactg
     6961 aacctgcagg tgcagcaggc cctcagctct gtagaaggag tgaggaacca gttgggagaa
     7021 tacaatgaac tgctacaggc caatgcagct aagctggccg aagcagatga agcttttaag
     7081 ataacagagg ctgcttacaa ccaagccacg cagcaataca atgactttaa cctgcaggtg
     7141 actaagcagc aaagtaagat tgcagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnngatct gcgccagcaa atagaaaata atacagcgca
     7261 actggccgaa accagcagca acattgaaca gtcctcccaa tcattgaagg atatcgaaga
     7321 agccctggtg caactgatga aaagcaagga agaagaagaa ctgaaactga acgaagcaga
     7381 ccaggcttat tataatttcc gcaaccagct gagtgaaaaa gaaagtgaga tacgccacaa
     7441 ggtgaaggat aaagaacagg tggaacatat gctggcagcg atcaaggata aactgaatga
     7501 actgaagctg caactggccg gcatgaagga aagaatgaat gtggagttcc gcattgacct
     7561 ggaatccatc ctcggcgaag ccaggacagg ggaggtgccg gtagaagaat tgcaggagaa
     7621 ggcagaccgg atgaagaaga gactggagaa cctgggcgaa gtaaacccga ctgcgattga
     7681 ggcattcacc gagatgaaaa agcgatatga cttcatcgtt gaacaaaaga ccgacctggt
     7741 gaatgcgaaa gacagtttgt tgaagaccat tgaagaagta gaagcaaagg ctaaccagaa
     7801 gttcttagat accttccact tggtgagaga taatttccag aaagtattta aaacactctt
     7861 tactgaagat gatcaagccg acctggtttt ggaaaacccg gagaatattg cagaaacagg
     7921 tattgatgta gtggccaagc caaaaggtaa gcgtccctcc tctttaacac agctgagtgg
     7981 tggagaaaga acactgaccg caaccgcttt gctgtttgcc atttacctga tcaagccagc
     8041 accattctgt attctggatg aggtagatgc cccgctcgat gatgccaatg tgggcaagtt
     8101 caccaatatg atccgccagt tcagtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 cccacaataa aatgacgatg agtaccgtgg atgtgatcta tggggtgacg atgcaggagc
     8221 cgggtgttag taaactggtg ccggtggatt tccggaattt gcagcagaat cagaattagt
     8281 ctatagttaa tagtcaatag tattgagtcc ggtcatgccg gacttttttt taattgagct
     8341 gttttatgcg tacaatcatt ttactctttg cttccaatgt gttcatgact tttgcctggt
     8401 actatcattt aaagcaccag ggatggcctt tgtggaaggc gatattgata agttgggcaa
     8461 ttgccctgtt tgaatattgc ctgatggtgc ctgccaaccg tattggcaag gttgacggat
     8521 tcaatctctt tcagttaaag atgattcagg aagctatcac cttaatagtt ttttgtgctt
     8581 ttgctgtttg gatcatgaag gaaccttttc agtggcgcta tattgtcagc tttgtcttac
     8641 ttatgggggc ggtatatttt atgttcagca agcaaccggt ataaggatct aagccctttt
     8701 ttcatgccgg tgctgcagtt gttctcttgt tcgctgtctt ttaatgagat ccgattttac
     8761 tttatgtact gcggcactta ccaggaagta aagccctatg caaaggatga gggatgcaac
     8821 gatggcccag atataccagt catctttctg ttctttttca aatataggaa acaacagcag
     8881 gaagatactt ccggttacaa aagagaaacc gagcaggagg ttggtaacgt atttatcact
     8941 gtgagaattg ggcatgagaa gtatttggtt tttatgggac ggaataccgt caacaatggc
     9001 tggcaaaaat agggaaaaaa taatcttgtg ttaatcagtt gacaacataa gacatctgct
     9061 gttacaacaa gtatttttgt aatataatca gtaagtgtat gaagaaaatc attgccgctt
     9121 ttttatcggt atttatgttg cacatcgttt ccgcacagga agcaccggaa ggcttgttta
     9181 tcggatccaa agcgccggac tttaaagcaa aagatcaaaa cggtaatgaa gtacgattaa
     9241 aagacctgct gaagaaaggc aaagtggtgc tggtgtttta tcgtggtcaa tggtgccctt
     9301 actgcaacag ggaattgtcc cgcattcagg attcattaca gctgataaaa gacaaaggtg
     9361 caaccgtagt agctgtttca ccggaaaagc aggagagtat tgctgctaca gtagagaaaa
     9421 caaaagctga atacccggta ttatatgatg agggactgaa gatcatgaaa gcctatgatg
     9481 tagagtttga agttccggag aataccatta tccgttaccg caatgctaat attgatattg
     9541 agaagaataa tggttccaac ggaaaatacc tgcccgtgcc tgccgtgtat atcatcgata
     9601 aagaatcaac cgttacctat cgcttctttg atacagatta caagaagaga ccatgggtaa
     9661 aagacctgct gaataattta taacgattag cgccagacga tctttcctat ctttcctttt
     9721 tcgccggcga agtaaatggc agcgccgagt ttggaagccc ttaccacatg aaagctttcc
     9781 ttactgatcc atttccagtt tttgccgcca tcgttggagt aatcaacccc gttcagaccg
     9841 caggcaaata catccgtttt agaaaagtat tccacacaac tgcgataccc atgtggtgga
     9901 gtatggggtg tttcccaggt ttgtcctcca tcattcgtaa agcagcagtt ttgcgaacgg
     9961 gatgaatcgg ctttgaaatc accgcctact acgatcatcc gcttgccgcc tttcatcttt
    10021 aatttatccc atacagcgat agaatttgcg cctgttgttt cctgtccctg tatgatggga
    10081 agtggggtgg ttttgtccct gataaataat cttgacttca caccaccgct tacaaaaacg
    10141 gcttcatccc tgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaaag caggcttcac
    10201 cgctgtcggc aacaggacgt ttgtcaaaag gaatatcctg ccagctgtta ccaccgtcaa
    10261 aggtgcggct gatgaaaaaa cggccattga tgggatcgcc gatcacaata ccggcttttt
    10321 cattccagaa ttccatggca tcgagaaaca tgcctttgtc tttgttctca taaactattt
    10381 tccagctttc gcctccatca atggttttta aaatataagc aggttcactt acggccataa
    10441 tgatagcggt tgatgcatca aaggcttcaa tatccctgaa ctctttattt tcaaatcctt
    10501 ttacgatcat ccatttccag gttttgccgc cattggagga acggcctacg gttcccttgc
    10561 tgccgcttac ccatatcaca ttatcattga ctacacttaa accacgaatg ctggtattgg
    10621 tgccggaagt gagtacttcc acagcagctc cctgtgcaaa gagagatacc ttgtacaact
    10681 gcattgctgt tactaaaaca aagcaaagta tatttttact ggtcatatgt ttcgcttgta
    10741 agattattga tagggggtca tggttctact gacttgtcct tcggggccca gttccattaa
    10801 ggcataagcg ggggcaaatt cctggaagga gccattccac cagtttccgg agacagcgcc
    10861 gttacaataa tatttaatac caagataatc cacttcatcc tgcaggtgaa tatggccgct
    10921 gatacacact ttgatattcg ggtattggcg gaaaatgggt ttcaggacaa gaaaatcgct
    10981 gtgcatcagg ttgcgtttaa tcatcaggtc gccattggcc tctgttttat taaaaaacaa
    11041 tcctgcacag atggataaga tgggaatatg cgaaatgata cagatgaact gcgtacttgc
    11101 ggtgtttttc aattcttctt ttaaccagat taattgctct tcatctaatt ttcctatata
    11161 acctccggcc gggttgagct gcgtactgtc cagcacaatg aatttccaat ttgcttttgt
    11221 gaagctgtaa aagcgtttag gcatttccag cgtttccacc acccacactt taccgtagag
    11281 ccggtcatta tcgggtttat tgcttttgat gaaccagccc cacacatcat ggtttccgat
    11341 ggtatgatac accgacaggc tgttttcttt ttgtagaatg cttttgaaaa gatcccactg
    11401 tgtttgcgtt tttgatttat ctgcttccag cgcatccatg atggcatcac cgccattgac
    11461 tataaaatcc actttacgat ttagttgttg tacatgctgc aaggccctgg ccataccggc
    11521 ttccggcaac aggcctggtt ttacatgtat atcggaaaga tgtgcaaacc ggatccattt
    11581 tctcttttct tccgctgccc ttgcaaagga gggaagcatt gacccgccaa tgacagcagc
    11641 ggtattttgc aaaaaggctt tccgtttcat aactatttca gttgtgcgat gaattcttct
    11701 ttgctgacga ttccctgttt tctccacact tcttttccgt tcttatagat gacaaaaacg
    11761 ggtatcggtt cgatgttcag ggctttcatt acatcagtgt gtatgccggc atctaccttg
    11821 atgagttgaa attgcagtga tgcatctttt tgtaagtcgt cgagtaccgg gttcattttc
    11881 acacaaggcg ggcaccagga agcaccaaag tcaaccaata ttgttttatc agccggtatc
    11941 atggcctggt aatcggcaat gctcatttgt tgttggttgc cgcctccttc aacaggttta
    12001 ttggcccttt tccatgcatt gataccaccc tgcaattcca ctaccttttt gtaaccattt
    12061 tcacggagcc agttagctgc tccggtactg cggcttccta caaggcagta cacatacacc
    12121 ggtttgtctt tgtcaatgta ttttactctt tcagtaaatt caggcgggtt cacccaattt
    12181 gcctgcaggg catttttaat atgaccggaa ctgaactcag cagcggtacg aacatccagg
    12241 atttgcacat tgggtgcgga tatgcctttt tcaaattctt cggcattgac tttgctgtta
    12301 ggacccgact gggattgaca tccggagaaa gtgaataata aagcaataac caaaacgttt
    12361 ttcattagat caattttctg gtacaaataa gtttcaaatg tagtgttttg tttttcatta
    12421 cccgggcagg ctaggcttcg tttcttttat gccctgcatc tgactggtaa gaaaaataat
    12481 cttattttta gcgattaaac agtaattccc cttttatgaa aaaacaactt ctttacttgc
    12541 tggctatcgg gctggttgtt attattggtt gccagaaaga gctgagcttt gagggtgaca
    12601 gtacacctgc agagggcagc ctgcaaagtg atattaacgg tgattgtctg cccaagtcag
    12661 taaacggggt ttatgtggcg gccaagccac tggcagcaga taccaatacg atttcagttt
    12721 cagtaaatgt tgtgaagaca ggttcttatg tgatcagttc tgatactgtg aacggttatt
    12781 ttttccgggc tacaggcatt ttcaccacac ccggtgtaaa cacagtaacc ctgagaggaa
    12841 acggaacacc ctttgctgcc ggtgtaaaca attttgtcat cacttttgac agtacagttt
    12901 gcgatataca agtaacagtg ttaccagcag gcgccggtgg accagcaacc tttacccttg
    12961 ttaatggtgg aacaccgccc aattgtgctt ccgcggtggt gaacggaacc tatactaagg
    13021 atgttgccct tacagctgcc aatactgtag atgttacggt aaatgtaacg gctatcggaa
    13081 catataacat tagtgcaaca ggtggtggaa tgacttttac gggtactggt gcttttataa
    13141 cattaggtaa ccaaactgtg accttaacgg gttcgggaac acctactact actggtgcaa
    13201 acacggtaac atttgcagct ccttttgcct cctgcaattt tacggttaat gtaactggcc
    13261 cttgtgaggg aacattaggg ggtggacccg gtgcctgtac gccttcaatt gtcagcggga
    13321 tatatgtggt aggttcttcg ctttcaggcc cgggaaataa tgtgcagata caagtaagta
    13381 ttactaccgc aggtgcttat aatatttcta ccaacacggt ggcaggtatt actttctccg
    13441 gcaccggaac aattgctgtt ggccctaacc agaatattaa cctggtggnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn naacgtactg ccgaacgatt atttcccgag aacaaccaac agcaactgga
    13621 gttatgagtg ggataatgac cccacggatt ccctatacag atttgcaatc tcggcaacaa
    13681 atgcaaccgc atttggcaca ttcaatattt ttatggagaa cgatggcact gctccgccgg
    13741 cgggtgattc atccggctat tacaggagaa gcggcggtga ttattttgaa tattttgacc
    13801 ttagtgctat tgggttagat aacccgcagt gggtggaata tactttcctg aaagataatg
    13861 tagcagccgg caattcctgg acatctaccg ggtggaccaa tgccttaggc ggtacgccgt
    13921 ttactttccg ttttaaggat agtatcgaac aaaaagatgt ggctgtgccg caaactacat
    13981 cccttggtac agtaaactac cagaatgtaa tagtagtggt tgaacgtata caactgcagg
    14041 tagggccagc atgggtagat gcttcttcag tgtttggtta tgccaaatat tactatgcaa
    14101 gaggtattgg actcataaga attgaattat ttgatcctcc tggtacaagt ccaacgccat
    14161 catttgttca gcgattgcga agacaccagg tattttgatg tattaataca cgaagaaggc
    14221 tgccgatggc agccttcttc gtgtatccga taataatatc agaatccttt tttctttccg
    14281 gactccaatg gtggaagttt gaaatcatcc acagcacttt tttcgactat tttcttttcg
    14341 atgtcttcta ttttgcgggg tgcaaaatgg gagagtagtt catacccggt ttcggtgatc
    14401 agcaggtcgt cttcaatccg caccgcaatg ctccaccatt ttttatcaca attactgcct
    14461 tcaggtatgt agatacccgg ttcaatcgta atcaccatat cttttttcaa cttatcataa
    14521 gtgcccctgt catgtacatc cataccaata tgatggccca gtccatgggg gtaatattta
    14581 ctcacttcat ttttattctt aatgatgccc aggtttaata agccctctgc aattacctgt
    14641 tgggctgcag cgctggcatc tgcccatttg gaaccatcct tcaaagtctt aaaagcggct
    14701 tcctgggcat cgtacaccaa ctggtatatt tgtctttctt cattcgaaaa ttttccatcc
    14761 actggaatgg tacgggtcac atcagcggta tagccatgat attctgcacc tacatccatc
    14821 agcagcatgc tggtgcctac cttggtcttg ctattttcca tatagtgcaa tatgcatcca
    14881 ttctcaccgg caccgataat tgaaccgtat ccgacacttt ctgcaccgta tttcttatgc
    14941 acatattcat gcagaccctg tatctccagt tccgacatat tagggttaat ggct
//
LOCUS       DAORLS010000034        14888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2157_length_14888_cov_2.030405, whole genome shotgun sequence.
ACCESSION   DAORLS010000034 DAORLS010000000
VERSION     DAORLS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2157_length_14888_cov_2.030405"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>301)
                     /locus_tag="PK977_04380"
     CDS             complement(<1..>301)
                     /locus_tag="PK977_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HRF17376.1"
                     /translation="LVNACDKLLINCYPFWEGANIEHAGIYLQEMYNKVKAIANGKEV
                     IITETGWPSNGQVVGEAVPSKENLMLYYMEVQLWAVQANVKLFYFSSFDEAWKIHF"
     assembly_gap    302..311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<312..>1991)
                     /locus_tag="PK977_04385"
     CDS             complement(<312..>1991)
                     /locus_tag="PK977_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF17377.1"
                     /translation="TFNFFYFLFFKLCLYVGLTIFSIPFVAMGYEMSEDFHERTQIMA
                     ISQWIGQWAWVIVPWFWIILYDKNWFATPESGARELSVWVAVICMLFAMVPAFFIKGK
                     STLHETSFKPIGIRNLKGIFTSLLHEFKEPFKIKDFRKLCYSTFFTFNAFNTVAQFTY
                     FIIVYYLFNGDQKNGSVWAAVFGSGGALVTTFIVIPVVVKMSKLIGKKRAFLLSQAIS
                     IFGYIILFFLFVPGKPWLFTIALPFFSFGIGGLFTTMMSMTADVCDVDELNYGQRREG
                     IFGAIYWWVVKLGFAFAGLLSYLILAAINFDGNLPTQTPETIFWLRFFFSAIPIVGTL
                     VAIYFMWDYGISEEKHKSIQQELAKRKAPKVSGSGISNIFSGINLKGLTKQDLLKRYP
                     SYINTGIDIETITIEGIKEKFNEIFNKGMHGISFTVFTEDQNPGDQISEEQIVKRLKV
                     LVGHTEWIRIFSTTSGYELIPSIAKKMGFKVLIGAWIGKDGEQNAKEVKTLIKLINEG
                     SADMAAVGNEVLFRGDQDEATLINYISEVKKNTSGVPVCCVDIYNEFINHP"
     assembly_gap    1992..2122
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2123..2326)
                     /locus_tag="PK977_04390"
     CDS             complement(<2123..2326)
                     /locus_tag="PK977_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17378.1"
                     /translation="MSNTNRTYQVPMYKKVAFGVGMLANQMFPAALGVFMVVLVNGFG
                     FPVWMVGILTFAPRLLDFIIDPIM"
     assembly_gap    2514..2690
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2691..3776)
                     /locus_tag="PK977_04395"
     CDS             complement(<2691..3776)
                     /locus_tag="PK977_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17379.1"
                     /translation="MQVVETSAAGNKLTPVNQAAPTANALTITINPEQRFQKITGFGG
                     SFTEASAYLLNKLSETNRDKILKAYFSEEGANYSLTRTHINSCDFSLSNYAYANVAGD
                     KTLQHFSIDEDRNDLIPMIKGAKKLSKEGFKIIASPWTAPPWMKDNNDWRGGKLLPEY
                     RKTWALYFSKYIEAHKKEGIDIWAVTVENEPLGNDKNWESMHFSPEVMTEFVKDYLGP
                     HFENNGLASVKILGYDQNRGEELEKWANVMYKDEASSKYFDGMAVHWYASTFDFFPAS
                     LQHTYKLAPDKHLINTEACVDADVPHWKDDNWYWNKEATDWGWDWASEKDKYLHPKYV
                     PVFRYAGDIIGCLNNYVDGWIDWNMVLN"
     gene            complement(3870..>7320)
                     /locus_tag="PK977_04400"
     CDS             complement(3870..>7320)
                     /locus_tag="PK977_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17380.1"
                     /translation="YQRNNITTGNSEITLSEVKMGNEIFYKISNADKMRPFFMSIVSD
                     SNHWLFVSSNGGISAGRKNAEYALFPYYTDDKITDSAEITGNKTIIRINIKGELTVWE
                     PFSVRSEGRFAISRNLYQNRFSNKIIFEEINHDLGLNFQYEWNSSNLYGFVKKSRLLN
                     QSGKSIQFEILDGLQNIMPYGVPSDLQTKTSNLADAYKRSELVAETGLGIYALSAIIV
                     DKAEPSEALKANVTWSLGLERPLYLLSALQLQHFRNGKTIKQEEDVKGEKGAFFIISS
                     LTLQAAEEKEWITVANVNQNHSALVQLSEAIKKDKELLKKVLDDVELGSERLQQLIAA
                     SDGFQYTADNFKDTRHFSNVLFNIMRGGIFDNNYQIEKWDFNVYLKKANKKVFEEAAV
                     YINRLGDNFSVFELKAVAQQSGNNDFIRLATESLPLKFCRRHGDPSRPWNQFSINTTN
                     KTDGSKILDYQGNWRDIFQNWEALAHSYPAFIEGMIYKFLNASTFDGYNPYRVTKDGF
                     DWETIEPDNPWSYIGYWGDHQVIYLLKFLEFAEQQKKGAIALLLSKNDFVYANVPYRI
                     KSYTEIVKNPKDTIAFDHNNEKNIHERINTIGADGALLTNTKNEICHVNFLEKILAVV
                     LSKLSNFIPEGGIWMNTQRPEWNDANNALVGNGVSMVTLYYLRRFLKFLEPVFQNANE
                     QDISISSELYTFYTELAHTFTKHSAVLSSAIDDKVRKQITDALGTAGSNYRNQIYSQK
                     FSGEKKEISVNELSEFVKTCLQYLEHSINANKRKDNLYHAYNLLSCYETGIKISHLSE
                     MLEGQVAVLSSGYLSPDESLQLLDALRQSSLYRKDQDSYILYPNKALPGFLQKNNLPK
                     QAVESSALLSMLIADGNFSIIDKDINGVYHFNGNFRNAEDVKQVLHKLGAKYQLQVAK
                     EREQVLQIFEEVFNHKEFTGRSGTFYGYEGLGSIYWHMVSKLALAVEETCLRAISENA
                     KEDTISRLIEHYYEINEGIGVHKSPELFGAFPVTPYSHTPMHRGAQQPGMTGQVKEDI
                     LCRIGELGVQVQDGCLYFHPQLLCRNEFIKGEKEVEITDVNGNKQVIAIEKDALLFTY
                     CQVPVMYIISNSEGVDIERADGSKNSYGHFVLNETDTASVLNRTGAVKQIRVHIQESR
                     LIK"
     assembly_gap    7321..7371
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7401..8249)
                     /locus_tag="PK977_04405"
     CDS             complement(7401..8249)
                     /locus_tag="PK977_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRF17381.1"
                     /translation="MILSIYRRLKNTLLTGVVCTTLVFAMSSCEREAVQTLPQREWEL
                     VWSDEFNGTAGNAPDAAKWAFDIGTGSNGWGNSELQYYTSRPANVQLDGSGKLVITAR
                     SESYAGSGFTSARIKTKGLFAQAYGRFEARIKTPTGPGIWPAFWMLGANIDTKPWPQC
                     GEIDIMEQRGQQPSVTHGTVHGPGYSGGNSIGKPYALTSGRFDTDYHIYAVEWGEDYI
                     DFFVDNFLYQRITPEDVPGEWVYNQPFFLLLNVAVGGNFVGFPTTGTPFPQSMYVDYV
                     KVYKQK"
     gene            complement(8283..10658)
                     /locus_tag="PK977_04410"
     CDS             complement(8283..10658)
                     /locus_tag="PK977_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17382.1"
                     /translation="MKKNQSINNVLFLLVGITTLIGMGCQKRDITDLQSPSFPNTAEV
                     FIDDFTSDLAYAAFGTSDVKAFQVDNQVSYGGTRQSMRFDVPDANSPNGSYAGGVFLS
                     KTGRNLSGYNAITFYIKASQPATIGSLGFGNDFGANKYLVTLSALTVNSNWKKVIIPI
                     PDASKLTQEKGLFWYSTGPENNRGYSFWIDEVKFEKVGDLGQATATIINGVDRAQVSY
                     VGVTNTLDGFSATYNMPDGVNRTINLTPYYFKFSNTNPAVASVTENGIVTTIGGGTTK
                     ITATLDTIHARGSLTLNSAGAYVGAPTPTRDPANVTSVFSDAYTNINVDYYNGYWAPF
                     QTTQSADFQVGNDRVLNYTNFNFVGIQVTSPTVNIGNRSNLHLDLYFPDNINPGATFK
                     VRVLDFGANGAPGGGDDVSDIVTLTNPALAARTWLSFDIPFSSLPGLATKGHFGQIIM
                     EGTNISNFFADNIYFWSNPVVPPVPAPVPTRPSANVLSVFSDSYTNLAGSDFFPNWGQ
                     STVVTQIPIGGNNTLRYSNFNYQGLQLAANQNLTSYQFLHIDYYSSNAGTLNAFLISP
                     PGSPGGTAPWEKAFSLSVPTAGWHSIDIPLSHFTPQVALSSVFQFKFDGGTGSDIFLD
                     NIYFWRIPPVPTVAAPVPTYPAGGVISIFSDSYTNVPGSDLNPNWGQATVVTQTPIVG
                     NNTLVYTGLNYQGLQFGSNQDVSGKQFLHLDFYSANSTALNVYLICPPGSPGGTAPWE
                     KAYALTVPTANGWNSVDIPLSHFTPQVALNNVFQLKFDGNGTIYLDNILFR"
     gene            complement(10692..>11247)
                     /locus_tag="PK977_04415"
     CDS             complement(10692..>11247)
                     /locus_tag="PK977_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17383.1"
                     /translation="HLPTIQDAAIGILANRTLFAFPGSAPAQDLWETHVRVVSKVNPH
                     LGLIANLYAGNGQANGNDKRTIDRYGFDLRTIYKKTKLIAIGKFNDWGPFDYHRDFNL
                     TFPLQLIADISTEIGKPDWFMLPGTKIGIRGTYRTLDRYSPRYSPIKTMDAAGNWVPD
                     PTAIGFPDGNEWEIRTYIQINIGK"
     assembly_gap    11248..11308
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11309..>12859)
                     /locus_tag="PK977_04420"
     CDS             complement(<11309..>12859)
                     /locus_tag="PK977_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17384.1"
                     /translation="QFSDGWWKTGQTLNLDEHDVTASWSNGGYLNDFMTGENNMNEEW
                     FGICAKGKTNERGTYELYPRAAYYALKEVHQFNPYISGAGIAYFDRIQIADAALRARG
                     DKAAMQAEDKGKLYLSDLQANFSTYNTGGSLISTPKQNEPVVPNTFPNRLGFDHMQSF
                     NIGVTARPAPNMMANVQFNVLGNVAENPIDEVFYENRGRRITVNTPTGQQQINSNNRI
                     QLYHASYSWDAKLFKLTGFYRTGHYHWGYEGDFFGLYPEANYGPNIDIYNGEAPFGFE
                     IEGKKAIKGLKLAFGPELWWGANPAILAKYTRKVKGFDITGMFHEDLTQRSGLQSSFA
                     VPMPKTRKAAISVSRKFGSFGFDLGGLWAGQPLNGRVFQLMREGNVYQDVIKSEDNFG
                     GKIKFTYSAGIVNWYGLASYMGLVANGGVDQTRTFTGWRLKDVGSGNMYNVLSGFTVS
                     LGKLQIAPNFLYQKPIEGPIPSDVLAPGRPRNILDDPFSVRSNRETIAGELLLTYDPT
                     PATWMYEW"
     assembly_gap    12860..12886
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12887..>13240)
                     /locus_tag="PK977_04425"
     CDS             complement(<12887..>13240)
                     /locus_tag="PK977_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF17385.1"
                     /translation="HLYKLFNDAVKEMKAIDNNHPMAICNGDLLFLDIIAKECTDIDI
                     LGINTYRGLTFTDLYDRVKKEFGKPVVLTEFGSDAYNTKANSEDQEYQAKVLVSNWKE
                     IYANAAGMGKNGNSLG"
     assembly_gap    13241..13263
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13264..13818)
                     /locus_tag="PK977_04430"
     CDS             complement(<13264..13818)
                     /locus_tag="PK977_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRF17386.1"
                     /translation="MRKLVALFALLFLGFFFAIAQPGKITVKRSGDGFVLEAQGKDFF
                     INGMNWDYFPVGTNYSYSLWNQPDDVIRAALDYEMPLLKNMGVNAIRQYTGVPARWIK
                     YIYENYGIYTMLNHSFGRYGLTLKGKWEVNTDYSNPLVRELLLKEVKEMVNEYKNTPG
                     LLLFLLGNENNYGLFWRGAETENIP"
     gene            14169..>14888
                     /locus_tag="PK977_04435"
     CDS             14169..>14888
                     /locus_tag="PK977_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17387.1"
                     /translation="MTSFTRSRYGAGTQNWAIERDEKNRLYIANNEGLLVYNGTIWQL
                     YPVPNKTILRSIGFGPGGRLYAGAQDELGYYAPDEVGRLRFTSIKKLLPEQDRNFTDV
                     WQLEVADKDVFFRTDAKVFKLSGEKITIYPTNSAWLSMHKYQENILAHEKNTGLLLYQ
                     NNQWQPFISRDLLPPDFFITDIVTYHKDTSLLSTISNGLYMLTKNKLIPFSIKSSLLN
                     TNQQFTSLWMLEDNSFLAGTYF"
BASE COUNT         4188 a         3152 c         2641 g         4427 t
ORIGIN      
        1 aaaatgaatt ttccacgcct catcaaaaga agagaaatag aatagcttta cgttggcttg
       61 cactgcccac aactgtactt ccatataata caacatcagg ttttctttag atggaacagc
      121 ttcacccact acttgtccat tacttggcca accggtttca gtaataatta cttcttttcc
      181 attggctatc gcttttactt tattgtacat ttcttgcaaa taaatacctg catgttcaat
      241 attagcacct tcccaaaacg gatagcagtt aatcaatagt ttatcgcatg catttaccag
      301 cnnnnnnnnn ntaggatggt tgataaattc attatagata tcaacgcaac aaacaggtac
      361 accacttgta ttcttcttca cttcgcttat ataattaatc aacgtagctt catcttggtc
      421 tcccctgaat aaaacctcgt tgcccacagc agccatatct gcagaacctt cattaataag
      481 ctttatcaga gtcttaacct cttttgcatt ctgctcacca tccttgccaa tccaagcgcc
      541 aattaaaact ttaaaaccca tttttttagc tatactcgga atgagctcat aaccgcttgt
      601 tgtggaaaat atccgaatcc attctgtatg accaacgagt accttcagtc gctttactat
      661 ttgttcttca gaaatctggt cgccggggtt ttgatcttcg gtgaataccg taaaggaaat
      721 gccatgcata cctttattaa atatctcatt gaacttctct tttatacctt ctatagtgat
      781 agtttcaata tcaataccgg tattgatata cgaaggatac cttttcaaca aatcctgctt
      841 ggttaagcct tttaaattta taccagaaaa aatattgctg attccagatc ctgaaacctt
      901 tggtgccttt ctctttgcca gctcctgttg aatgctttta tgtttttctt cagaaattcc
      961 ataatcccac ataaaataga tagctaccaa tgtacccaca ataggtatgg ctgaaaagaa
     1021 aaatcttaac caaaaaatag tttcaggtgt ttgcgttggc aaattgccgt caaaatttat
     1081 tgctgctaaa atcaagtagc ttaataaacc tgcaaaagca aaacccaatt ttacaaccca
     1141 ccagtaaatg gcgccaaaaa tcccttccct tctttgtccg taattgagtt catctacatc
     1201 acatacatca gccgtcatac tcatcatggt tgtaaacaaa cctcctattc caaatgaaaa
     1261 gaacggcaga gcaattgtaa acaaccatgg cttaccggga acaaaaagga aaaacaatat
     1321 aatataacca aaaattgaaa tggcttgtga taataaaaat gcccttttct ttccaatcag
     1381 ctttgacatc tttaccacta ccggaattac aataaaggtg gttaccagtg cacccccgct
     1441 gccaaaaaca gccgcccaaa cactgccgtt tttttggtct ccattgaaca gatagtaaac
     1501 aatgataaaa taggtaaact gggcaaccgt attaaatgca ttgaaagtaa aaaaagtgga
     1561 atagcacagt tttctgaaat cttttatttt aaatggttct ttgaactcat gtaaaaggct
     1621 ggtgaaaatt ccttttaaat tacgaatacc aataggttta aatgaagttt catgcagcgt
     1681 tgacttgcct ttaataaaaa atgctggcac catggcaaaa agcatacaaa ttacagctac
     1741 ccataccgac agttctctgg caccagattc aggtgtagca aaccaatttt tatcataaag
     1801 aataatccaa aaccagggaa caataaccca tgcccattgc ccaatccatt gggatatggc
     1861 catgatttga gtccgctcat gaaaatcctc gctcatttca tatcccattg ccacaaaagg
     1921 aatactgaaa atggttagtc ctacatacag gcatagttta aaaaagagaa agtagaaaaa
     1981 gttaaacgta annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nncataatcg ggtctataat aaagtccagt aaccgtgggg
     2161 caaatgtcag aatacctacc atccataccg gaaagccaaa gccgtttacc agcactacca
     2221 tgaaaactcc gagagcagcc ggaaacattt ggttggccag cataccaaca ccaaaagcaa
     2281 cttttttata catgggtacc tggtaagtgc ggtttgtatt actcatatgg cagataatta
     2341 agcttggtta gaaaactatt tttttattac aatcgtttga atggcttttg cacttatcgt
     2401 tgcttttaca ttttcttttt gcaatgtaat gctgaatgct ttttctgttt cagttggatt
     2461 aaacactact accgccacag acccatccgg attttgtgca gcagttacct gcannnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attgagcacc
     2701 atgttccagt caatccatcc atccacataa ttatttaaac agccaatgat gtcaccggca
     2761 tatcgaaaca ccgggacata tttcgggtgc aggtatttgt ccttttcgct tgcccaatcc
     2821 catccccagt cggttgcttc tttgttccag taccagttat catctttcca atgcggcaca
     2881 tcagcatcta cacaagcttc agtattgatc aaatgtttgt caggtgccag tttgtatgta
     2941 tgctgaaggg atgcagggaa aaaatcaaaa gtgctggcat accaatgcac cgccatacca
     3001 tcaaaatatt ttgaagacgc ttcatcttta tacatcacat tggcccattt ctccagttct
     3061 tctccacggt tctgatcata acccaaaatc tttactgatg ccaatccgtt attttcaaaa
     3121 tgaggaccca aataatcttt tacaaactct gtcattactt ctggtgaaaa gtgcatactc
     3181 tcccagttct tatcattacc cagcggttcg ttctccacgg taacggccca gatgtcaatc
     3241 ccttcctttt tatgcgcttc aatatatttt gaaaaataca aagcccatgt ttttctgtat
     3301 tccggcagca gttttccgcc ccgccaatcg ttattatctt tcatccatgg cggtgctgtc
     3361 cagggcgatg caattatttt aaatccctct ttggaaagtt ttttggcccc cttgatcatc
     3421 gggatcaaat catttcggtc ctcatcaatg ctaaaatgtt gtaatgtttt atccccggct
     3481 acatttgcat aagcataatt acttaatgag aaatcacagg agttgatatg tgtacgggta
     3541 agtgaatagt tagctccttc ttcactaaaa taggctttga gaattttatc ccggttggtt
     3601 tcgcttaact tattcagaag ataagccgat gcttccgtaa aagaaccacc gaagcctgtg
     3661 attttttgaa acctctgttc aggattgatt gtaatagtca gtgcattggc agttggtgcc
     3721 gcctgattta caggtgttaa tttgttgcca gcggctgatg tttcaacaac ctgcacagac
     3781 cataaattat tttgtgaagc agcaaccatt gcaatacata taaaactgat tggcaaaaaa
     3841 ataattttac ttctcacttc ctccaggttt tattttatta accgtgattc ctgtatatgc
     3901 acccttattt gtttaacagc tcctgtacgg ttcaataccg atgcagtatc tgtttcattc
     3961 aatacaaaat ggccatatga attttttgag ccatcggccc tttcaatatc aacaccttca
     4021 ctattactta tgatatacat cacaggcacc tggcagtacg taaacagcaa agcatccttc
     4081 tcaatagcaa taacttgctt attgccattt acatctgtta tttctacttc tttttcgcct
     4141 tttataaatt catttctgca aagcagttgc ggatgaaaat ataggcagcc gtcttgcacc
     4201 tgcacaccca attctccaat tcggcaaaga atatcttctt ttacctggcc tgtcataccg
     4261 ggctgctgtg cacccctgtg cattggtgtg tgagaataag gtgtgacagg aaatgcgcca
     4321 aacagttccg gtgatttatg cacaccgatg ccttcattga tctcataata atgttcaatt
     4381 aaccggctta ttgtatcttc ttttgcattt tcgctgatag ctctcaaaca ggtttcctcc
     4441 acagctagag ctagtttgga aaccatgtgc cagtaaatgg agcctaatcc ttcataccca
     4501 taaaatgttc cggaacggcc ggtaaattct ttatgattaa atacttcctc aaaaatctgc
     4561 aatacctgtt ctctttcttt tgcaacttgt aactggtatt ttgcccccag tttatgtaat
     4621 acctgtttta catcttctgc gtttctgaag ttgccgttga aatgataaac accgttgatg
     4681 tctttatcaa taatagagaa attaccatct gcaataagca tgctgagcaa tgcagaactt
     4741 tcaactgcct gtttaggcag attgttcttc tgtaaaaagc cgggcagtgc tttattggga
     4801 taaagaatgt aactgtcctg gtctttacga taaagtgagc tttgtcttag tgcatctaaa
     4861 agttgcaaac tttcatctgg agaaagataa ccggaactta acactgccac ctgcccttcc
     4921 agcatttcac ttaagtgaga tatttttata ccggtttcat aacaactcag caaattgtag
     4981 gcatggtata aattatcttt tcgtttattg gcattgatag aatgttcaag gtactgcaaa
     5041 caagttttta caaattccga taactcatta acagaaattt ctttcttttc tccagaaaac
     5101 ttttgcgaat agatttgatt cctgtaattg ctacctgctg ttcccaaagc atcggttatt
     5161 tgcttcctta ctttatcatc aatggcagac gatagaacag cagaatgttt ggtaaatgtg
     5221 tgtgccaatt ctgtatagaa agtatacaat tctgaagaaa tacttatatc ctgttcattt
     5281 gcattttgga aaacaggttc caggaatttt aaaaatcttc tcagataata caaagtcacc
     5341 atagaaactc cgttgccaac cagtgcattg ttggcatcat tccattcagg cctttgtgta
     5401 ttcatccaga tgccgccttc aggaataaaa ttggaaagtt tcgataagac aactgctaaa
     5461 attttctcca gaaaatttac atggcagatt tcattttttg tattggtaag cagggcgcca
     5521 tctgccccta ttgtgtttat cctttcatga atattttttt cattattgtg atcaaacgct
     5581 attgtgtcct tggggttctt tacaatttct gtgtatgatt taatacgata gggcacattg
     5641 gcatacacaa agtcattctt gctgagcagt aaagctattg ctcccttttt ttgctgttca
     5701 gcaaattcta aaaatttcag gaggtatatt acctggtgat caccccagta gccaatatag
     5761 ctccagggat tatccggctc aattgtttcc cagtcaaacc catcttttgt aacacggtaa
     5821 ggattataac catcaaaagt ggaagcattg aggaatttat aaatcatccc ttcaataaaa
     5881 gctggatatg aatgtgccag tgcttcccag ttctggaaaa tatcccgcca gttgccctgg
     5941 taatctaata tttttgaacc atcagtttta ttggtcgtat taatggagaa ttgattccag
     6001 gggcggctgg gatcgccatg acgacggcag aattttaacg gcagggattc tgttgccagc
     6061 cggatgaaat cgttgttacc tgattgttgt gcaactgctt tcagttcaaa tacagaaaaa
     6121 ttatcaccca gcctattgat ataaacagcc gcttcttcaa aaactttttt atttgctttc
     6181 ttcaggtaga cattaaaatc ccatttttca atttgatagt tattatcaaa tatgccaccc
     6241 cgcataatat taaagagcac attggaaaaa tgcctggtat ctttaaaatt atctgccgtg
     6301 tattgaaatc cgtctgacgc agctattaat tgctgtaatc gttctgaacc cagttctaca
     6361 tcatccagca cttttttcag taactcttta tcctttttaa ttgcttcact caattgtaca
     6421 agtgcagaat gattctggtt aacattggca actgttatcc attccttttc ctcagctgcc
     6481 tgaagggtaa ggctgcttat aataaagaaa gctccctttt caccctttac atcttcttcc
     6541 tgtttaattg ttttaccatt cctgaaatgc tgtaattgta atgcagaaag cagataaagc
     6601 ggccgttcta atcccaaact ccaggtcaca tttgctttca atgcttcact tggttctgcc
     6661 ttatcaacaa taatggcact taatgcatat atacctaacc cggtttcggc aacaagctct
     6721 gaacgtttat aggcatcggc taaattgctt gtttttgttt gcaggtcact gggtacacca
     6781 tatggcatta tgttttgcaa gccatccaat atctcgaatt gaatgctttt accagattga
     6841 ttcagcaatc ttgatttttt tacaaaccca taaagattgc ttgagttcca ttcgtattga
     6901 aagttcaatc ccaaatcatg attgatctct tcaaaaatga ttttattgct aaacctgttc
     6961 tggtataaat tccggctgat agcaaacctt ccttcgctgc gaacagaaaa tggctcccac
     7021 accgtcaact cccctttaat attaatacgg attatggttt tgtttcctgt aatttctgcc
     7081 gaatctgtta ttttatcatc tgtgtaatag gggaataagg catactcagc atttttacgg
     7141 ccagctgaaa ttccgccatt acttgataca aacaaccagt gattggaatc acttacgatc
     7201 gacataaaaa agggacgcat cttgtctgca tttgagatct tgtaaaaaat ctcattaccc
     7261 atttttactt cgctcaaagt tatttcactg tttcctgttg tgatattgtt gcgctggtaa
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacatgctat
     7381 gtttttagga aaaagaattt ctatttctgc ttatacactt ttacataatc cacatacatg
     7441 gattgaggga acggcgtacc ggttgttgga aagccaacaa aattgccgcc cacagctaca
     7501 ttcagtaata aaaagaaagg ctgattatat acccattcac ccggtacatc ctccggtgtg
     7561 atgcgctggt atagaaaatt atcaacgaag aaatcaatat aatcttcccc ccattcaacg
     7621 gcatagatat gataatcggt atcgaatctt ccgcttgtca gtgcataagg cttacctatc
     7681 gaattgcctc cggagtaacc cggtccatgc acggtgccat gcgtcaccga tggttgctgg
     7741 cctctctgct ccataatatc aatttcgccg cattgaggcc atggttttgt atcaatattt
     7801 gcacccagca tccagaaagc aggccatata ccgggacctg ttggggtttt tatccttgct
     7861 tcaaatcggc cataagcctg ggcaaataat cccttggttt ttattcttgc agaagtaaaa
     7921 ccagagccgg catatgattc gctcctggca gtaattacta atttaccgct gccatcgagt
     7981 tgtacattcg ctggcctgct ggtgtaatat tgtaattcac tgttgcccca accgttactg
     8041 cctgtaccaa tgtcgaaagc ccatttggca gcatctggtg cattacctgc tgtaccatta
     8101 aattcgtcac tccaaaccaa ttcccactcc cgttgaggca atgtttgtac cgcttctctt
     8161 tcacaactgc tcatagcaaa cacaagcgtt gtgcaaacta ctccggtcag taatgtgttt
     8221 ttaagtcttc tatatatgga taaaatcata caattaattt ttatggttaa ataatactag
     8281 cattacctga acaaaatatt atccaggtaa atagtgccat taccatcgaa cttcagctgg
     8341 aaaacattat tcagtgctac ttgtggtgta aaatgcgata atggaatatc cacactgttc
     8401 catccgttag cagtaggcac tgtcaatgca tacgcttttt cccatggtgc agtaccgcct
     8461 ggtgaaccgg gagggcaaat caggtaaaca ttcagtgctg tggagttagc agaataaaaa
     8521 tctaaatgca ggaattgttt acccgaaaca tcctggttgc ttccaaactg caagccctgg
     8581 tagttaaggc cagtatacac taatgtgttg tttccaacaa taggagtttg tgttactact
     8641 gttgcctgtc cccagttggg attaagatca cttcccggta cattggtata actgtcacta
     8701 aatatagaaa ttactccacc tgcaggataa gtaggaacag gtgctgcaac tgtaggtacg
     8761 ggtggtattc gccagaagta aatattatct aaaaagatat cactgccagt gccaccatcg
     8821 aacttaaact gaaacacact gctcaatgcc acctgcggtg taaagtgtga aagcgggata
     8881 tctatactgt gccagccagc agttggtaca gaaaggctga aagctttttc ccagggagct
     8941 gttccacccg gtgaacccgg agggcttatt aaaaaggcat tcaatgtacc agcgttagag
     9001 ctataataat ctatgtgcaa aaactggtaa gaagtgagat tctggttagc agctaattgt
     9061 aaaccctggt aattaaaatt actatagcgt aatgtgttat taccgcctat tggtatttgt
     9121 gttactaccg ttgactgccc ccagttgggg aaaaaatcag atcctgccag attggtataa
     9181 ctatcgctga atacagaaag cacattggcc gacggacgag taggtactgg tgcaggaaca
     9241 ggaggaacaa caggattact ccagaaataa atattgtcag caaagaagtt tgaaatatta
     9301 gttccttcca taatgatctg gccaaaatgt ccttttgtgg caagaccggg caggctggaa
     9361 aaaggaatat caaaactcaa ccatgttctt gctgccagtg cagggttagt caatgtaact
     9421 atatcactta catcatctcc gccacccggt gcaccattag cgccaaaatc taaaactctc
     9481 actttaaaag tagcacctgg atttatatta tcagggaagt ataaatccaa atgcaggtta
     9541 gacctattgc caatgtttac tgtcggagat gtaacctgga tgcctacaaa attaaaattg
     9601 gtatagttta ataccctgtc attacctacc tggaaatcgg cagactgtgt agtctgaaag
     9661 ggtgcccaat agccgttgta atagtcaaca ttgatatttg tataagcatc actaaaaact
     9721 gatgttacat tggcaggatc tcgggttggt gtaggagctc ctacataggc tccggctgaa
     9781 tttaacgtaa ggcttcctct tgcatgaatg gtatctaatg ttgccgtgat ttttgtagtg
     9841 ccgccgccaa tggtagtcac aattccattc tcagtaacag aggcaacagc cgggtttgta
     9901 ttagaaaatt taaagtaata gggagtgagg tttatagttc tgtttactcc gtcaggcata
     9961 ttgtaggttg cgctgaaacc gtcgagagtg ttcgttactc ctacataaga tacctgtgcc
    10021 ctgtccactc catttatgat agtggcggtt gcctggccca ggtctcccac tttttcaaac
    10081 tttacctcat ctatccaaaa tgaataacct ctgttatttt ccggaccggt agaataccaa
    10141 aacaatccct tttcctgggt aagctttgaa gcatccggaa taggaatgat tactttcttc
    10201 cagttactgt ttacagttaa agcactaagc gttaccaggt atttattggc accaaaatca
    10261 tttccaaaac caagtgaacc aatggtagca ggctgtgacg ccttgatgta aaaagtaatg
    10321 gcgttataac cagaaaggtt tctgcctgtt ttactcaaaa atacaccgcc ggcataagag
    10381 ccgttaggac tgttggcatc gggcacatca aaccgcatag attgcctggt accaccataa
    10441 cttacctggt tgtccacctg gaaagctttt acatctgaag taccgaaagc ggcgtaagcc
    10501 agatcagaag tgaagtcatc aataaaaact tctgcggtgt ttgggaaaga agggctttgt
    10561 aaatcggtaa tatcccgctt ttggcatccc atacctataa gtgtagtaat cccaacaagg
    10621 agaaacagca cattgttaat tgattgattt tttttcatac taatttcttt aaaattatta
    10681 gcaagcttat tttatttacc aatgtttatc tggatatatg ttcttatttc ccactcatta
    10741 ccatcaggga aaccaatcgc tgttggatca ggaacccagt tacctgctgc atccatagtc
    10801 tttattggag agtaacgagg tgagtaacgg tctaatgttc tgtaagttcc cctgatacct
    10861 attttagtac ccgggagcat aaaccagtcc ggcttgccaa tctcggtaga aatatcagca
    10921 atcaactgaa gcgggaaagt aaggttgaaa tcacgatgat aatcaaaagg tccccagtca
    10981 ttgaatttac cgatagcaat tagtttagtc tttttataga tagtacgcaa atcaaaacca
    11041 tagcggtcga tggtacgttt gtcatttcca ttagcctggc cattaccagc atataagttg
    11101 gcaattaaac ccaggtgtgg gttgacttta gatactaccc ttacgtgggt ttcccacaaa
    11161 tcctgtgcag gtgctgagcc ggggaaagca aataatgttc tgttggccaa tataccgata
    11221 gcagcatcct ggatggttgg taaatgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cactcataca tccacgtagc tggtgtagga
    11341 tcgtaggtca ataataattc tccggcaatc gtttcccggt ttgaccttac tgagaatgga
    11401 tcatccaaaa tatttctggg tctgcccggc gccagaacat ctgacggaat cggtccttct
    11461 atgggttttt ggtataaaaa gttaggagct atttgcagct tacccagact aactgtaaaa
    11521 ccactcagca cattatacat gttaccgctg cccacatctt tcaatctcca tccggtgaaa
    11581 gtccttgttt ggtctacacc tccattagcc accaaaccca tataggaagc caggccatac
    11641 caatttacaa taccggcaga gtaagtaaat ttgattttac ctccgaaatt gtcttctgac
    11701 ttaattacat cctggtacac attgccttct ctcatcaact gaaataccct tccattgagt
    11761 ggttgtccag cccaaagtcc acccaggtca aagccaaacg aaccaaattt tctggatacg
    11821 ctaattgcag cttttcttgt tttaggcatg ggcactgcaa atgaactttg taaaccactg
    11881 cgctgggtaa gatcttcatg aaacatacct gttatatcaa aacctttcac cttgcgggta
    11941 tattttgcaa gtatagccgg gttggcgccc caccacagtt caggaccaaa agcaagtttc
    12001 aaccctttga ttgctttctt tccttctatt tcaaaaccaa aaggtgcttc accattatat
    12061 atgtcaatat tgggtccata gtttgcctca ggataaaggc caaagaaatc accttcatag
    12121 ccccagtgat aatgtcctgt acggtaaaag ccagttaatt taaacaattt ggcatcccag
    12181 ctatagctgg catggtataa ttgaatcctg ttattggaat ttatttgttg ctgaccggtt
    12241 ggtgtattta ctgttattct tcttcccctg ttttcataaa atacttcgtc aataggattc
    12301 tctgctacat taccgagtac attaaactgt acattggcca tcatattagg agcaggtctt
    12361 gccgttacac ctatattaaa tgactgcatg tggtcaaaac ccaagcggtt gggaaaggta
    12421 ttgggaacta caggctcatt ttgtttgggt gtagaaatta aacttccccc tgtattataa
    12481 gtactgaaat tagcctgtaa atcgctcagg tataatttac ctttatcttc tgcctgcatg
    12541 gctgccttat cgcccctggc cctcaacgct gcatctgcaa tttgtattct gtcaaaataa
    12601 gctattccag caccacttat gtaaggattg aactggtgta cctcttttaa tgcatagtag
    12661 gcagcacggg gatataattc ataagtacct ctttcattgg ttttaccttt tgcacaaata
    12721 ccaaaccatt cttcattcat attattttcg ccggtcatga aatcgttgag gtaaccacca
    12781 ttggaccatg aggctgttac atcatgctca tccaggttaa gggtttgccc ggttttccac
    12841 catccatcac taaactggan nnnnnnnnnn nnnnnnnnnn nnnnnntccc agactgtttc
    12901 cgtttttacc catcccagca gcattagcat atatttcttt ccagttgctc accagtactt
    12961 tggcctggta ctcctggtct tcactattag cttttgtatt ataggcatca gaaccaaact
    13021 cggtaagcac cactggtttg ccaaattcct tttttactct gtcgtaaaga tctgtgaatg
    13081 taaggccccg gtaagtgttg ataccgagta tatctatatc tgtacactcc ttagcaataa
    13141 tatccaggaa aagcaggtct ccattacaga tggccatcgg gtggttgtta tcaatggctt
    13201 tcatttcctt taccgcatca ttaaataact tatataaatg nnnnnnnnnn nnnnnnnnnn
    13261 nnnaggaata ttctctgttt cagctcctcg ccaaaaaagg ccatagttgt tttcattacc
    13321 taataagaaa agcaacaggc cgggagtatt tttgtactcg ttcaccattt cttttacttc
    13381 cttcagtaac aactccctta ccaatggatt agaataatcg gtgttaacct cccattttcc
    13441 tttcaacgta agaccataac ggccaaaaga gtggttaagc atggtgtaga tgccgtagtt
    13501 ctcatatata tacttgatcc acctggccgg cacaccggtg tattggcgga tggcattcac
    13561 acccatgttt ttcagcaaag gcatctcgta atcaagagcg gccctgatta catcgtccgg
    13621 ctgattccat aaactgtagg agtagttggt acccaccggg aaataatccc agttcattcc
    13681 attaataaag aaatcttttc cctgggcctc taatacaaaa ccatcacctg accttttgac
    13741 ggtgattttt ccgggttgtg caatggcgaa gaaaaagcca agaaagagca gggcaaacag
    13801 tgcgactaat tttctcatat ggcaattttt atttgacaaa aaaaggggct gaaaatccgg
    13861 agatgctgtt ctatttatca atcaacaatt ttcagcttta tcctgtttcc ttatttatcc
    13921 ggatgaggat aaaaaggcat tttaaaagta attcctgaca agggacaagt ccaaaaaaaa
    13981 cgccacatca gtactactac agaaacaaaa aaaactatat ttagctttaa atcaatacat
    14041 cagtactaca tctgaagcca tattttagtt caaaaccagc ttattggaaa aaaacagctt
    14101 tgattttcca ggctgtaatc tgttgtgccc tcaacagtcc gtcccagggt ttgattggtc
    14161 aaaatgaggt gactagtttt accagaagca ggtatggagc gggtacccaa aactgggcta
    14221 ttgaacggga tgagaaaaac cgattgtata tagctaacaa tgagggcctg ctggtttata
    14281 atggtacaat ttggcagtta tacccggtgc caaacaagac cattttacgt tcaattggct
    14341 tcgggccggg cggcaggtta tatgccggtg cacaggacga actgggatat tacgccccgg
    14401 atgaagtagg aaggcttcgt tttacttcaa taaaaaaact gctgcccgaa caggatagaa
    14461 attttacaga cgtatggcaa cttgaggtgg cggataagga tgtttttttc agaacagacg
    14521 ctaaagtatt caagctcagc ggagaaaaaa ttactatata ccccaccaat tcagcctggc
    14581 tctctatgca caagtaccag gagaatattt tggcacatga aaaaaatacc ggcctgctgc
    14641 tttatcaaaa taaccaatgg cagccattca tttccaggga tttgcttcct cctgattttt
    14701 tcattacaga cattgtaact tatcataaag acaccagcct gctcagtaca atcagcaacg
    14761 ggttgtacat gctgacaaaa aataaactga taccgttcag tattaaatct tcattactga
    14821 acaccaatca gcaattcact tcactctgga tgctggagga taacagtttt ttggcgggta
    14881 cttacttc
//
LOCUS       DAORLS010000035        14888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2158_length_14888_cov_1.763231, whole genome shotgun sequence.
ACCESSION   DAORLS010000035 DAORLS010000000
VERSION     DAORLS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_14888_cov_1.763231"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>293)
                     /locus_tag="PK977_04440"
     CDS             complement(<1..>293)
                     /locus_tag="PK977_04440"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRF17388.1"
                     /translation="YGAISAFALHPIYINLQKVAGKEYAGQLKLLKKKQEELNTLSVV
                     DYEEVMKYKFSVLRELYNFMGVECLASEDFKVFYKENEYWLESFAAFCFFRDK"
     assembly_gap    294..330
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<331..1193)
                     /locus_tag="PK977_04445"
     CDS             complement(<331..1193)
                     /locus_tag="PK977_04445"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRF17389.1"
                     /translation="MKLQFYLRFHTKYGQSLWLTGNIEELGNNDMTKAIPLVYLNDEF
                     WTVSLDVKRKNFEKSFQYKYVLKNEEGELLNEWGNDRTGEAVGKEITEVHFVDTWNHA
                     GEYENAFFTAPFEKVLLKSTETKIKSSSDKHATHIFRVKAPLLKKGEAVCLCGNGSKL
                     GDWNTDKPSLLAREGNWWTVKLDLSEASFPLAYKFGVYNVKEKQFVQYEGGNNRLLYS
                     DASKGKYTVLHDGFVQLPNNTWKGSGVAIPVFSLRSKNGFGVGEFNDIKLLVDWARLT
                     GLKLIQLLPVN"
     gene            1323..1841
                     /locus_tag="PK977_04450"
     CDS             1323..1841
                     /locus_tag="PK977_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17390.1"
                     /translation="MNINYKSWKMLFLFCLGLFLGTAFCMKWMEKDLLQDNRLFTVIG
                     LEITYPQEKVYTILSGLDNKTRTILQYHLYFDFVFMSGVFPGIAALCMMARFKSTHRN
                     YRKILLAAAVSQIVAWLCDIIENNLLLSWVADPERIRMFDEFHLIVWVKWILAILGFI
                     LSIPVVFKTRTR"
     gene            1898..2053
                     /locus_tag="PK977_04455"
     CDS             1898..2053
                     /locus_tag="PK977_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17391.1"
                     /translation="MEQTKKYKGLWWLVFLASTAALLFAIYSHWEWLTLILPFQTTAF
                     VKAMDIM"
     gene            2158..2565
                     /locus_tag="PK977_04460"
     CDS             2158..2565
                     /locus_tag="PK977_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HRF17392.1"
                     /translation="MRKLLLNLAALLLFGSSINYCSAAVIPSTISSTNPDPATVKAAI
                     ENFKSLPRKERKERVKLAKKEIKAFNAAKKAGKEADTNTLLLVILAILLPPLAVYLHQ
                     GEINNKFWISLLLTLLFWLPGVIYALIVILGNG"
     gene            2672..3298
                     /locus_tag="PK977_04465"
     CDS             2672..3298
                     /locus_tag="PK977_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17393.1"
                     /translation="MKLFYISLFAFLVSCKSNRKPGANNDPGSINDKDSNTVSANKTK
                     TESTIIPGSPLAEKINTILGSMNESAAWHVLNDKEARWISGQFEYFIAPKRKDNPDYP
                     YIASGDFDGDKRPDLAAVVTDSTRVNYQLVIISDLDSSKKIKYWKEDIMEDAAISTAP
                     KGYIEGTLNEKVKKVKLESEAIHVEYFEKASFIIYWDGKSFKRIQQGD"
     gene            3325..3606
                     /locus_tag="PK977_04470"
     CDS             3325..3606
                     /locus_tag="PK977_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HRF17394.1"
                     /translation="MKKIITVFSVVALLSACSGAGSSGSDLAKEVCDCYQKANGMNAS
                     DPNRAKAQADCMASQGIAWNKIKDNQKEADEFNKIIGDCGKELIKKSVQ"
     gene            complement(3656..4000)
                     /gene="rplT"
                     /locus_tag="PK977_04475"
     CDS             complement(3656..4000)
                     /gene="rplT"
                     /locus_tag="PK977_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRF17395.1"
                     /translation="MPRSKNAVASKARRKKILKQAKGFYGKRKNVLTVAKNVVEKGMT
                     YMFVGRKLKKREYRSLWIARINAAVRAEGLTYSEFINKLQKKGIELDRKVLADLAMNN
                     PETFKSLVDSVK"
     gene            complement(4051..4248)
                     /gene="rpmI"
                     /locus_tag="PK977_04480"
     CDS             complement(4051..4248)
                     /gene="rpmI"
                     /locus_tag="PK977_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRF17396.1"
                     /translation="MPKVKTNSSAKKRFKVTATGKIMHQKSFKRHILTKKSKKRKRNM
                     RLDGTVAKSHVDFVKRLLRLK"
     gene            complement(4480..>5676)
                     /locus_tag="PK977_04485"
     CDS             complement(4480..>5676)
                     /locus_tag="PK977_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRF17397.1"
                     /translation="LMGQLIRFVSSHEVGHTLGLRHNYGSSSSVPVENLRNKAWVEAN
                     GHTPSIMDYARFNYVAQPEDKIDRSGIFPRIGDYDKWAIEWGYKQFLQYKSADAEKTH
                     LNNWVIEKLKDKRLWFGTETNPDDPRSQSEQVGDDAMKGSFYGVKNLQRIVPNLMEWT
                     KMPNEDFSGLSKIYNQVVGQYSRYMGHVAKYVGGIMETPKRVEEQGPVYEIVAEAKQK
                     EAVDFLNKQLFTTPTWLINQDIFGKTGLSGLTTIGGVQDNILGRLLSNRTFTKLLEAE
                     ATLGNNAYQMTELLGDLKKGIWTELATRKPIDIYRRNLQKSYINTLVSLVKPSSSGTT
                     TIGGITITTTSGTDKSDAKSVIMGHLAALRAEINAAAAGTVDLMTKYHLQDLAKRIDN
                     ALNPKD"
     assembly_gap    5677..5749
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5750..6970)
                     /locus_tag="PK977_04490"
     CDS             complement(<5750..6970)
                     /locus_tag="PK977_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HRF17398.1"
                     /translation="MLTFSFVTVIVYAQPPGLRPDSTRRPGSNNSGLKPYKEVITSRA
                     MSDAGLFWVHKVDDKYFFEIPDSLFGREILVVNRISKAAAGMRSSGSFFGYGGDQIGQ
                     NVIRFEKGPNNKIFLRNISFAEYTKDSTSDMFTAVNKSNIQPIAASFDIKSLGKDSSG
                     AVIDITDYISGDNDVLHFNGGLKSSLRLAALQQDKSYIVNVRSFPINIEIRAVKTYAR
                     SAAPSTTGGFGSATAAGNLTMELNSSLVLLPKVPMQPRYYDPRVGYFAVGYTDFDANP
                     QGVKSISMVKRWRLEPKDEDIEKYKRGELVEPKKPIIFYIDPATPKKWIPYLVQGVND
                     WQTAFEKAGFKNAIIGKMAPTKEEDSTWSLDDARNSAIVYKPSDIPNASGPSISDPRS
                     GEIMESHINWYHNVM"
     gene            complement(7169..7807)
                     /gene="infC"
                     /locus_tag="PK977_04495"
     CDS             complement(7169..7807)
                     /gene="infC"
                     /locus_tag="PK977_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRF17399.1"
                     /translation="MAVPQKPGGGFNRPSGGGGFKPGGGGFNKGGFNRGRFMPRKEAE
                     HRINHFIRVPQVRLVGENVEVGIYDTQVALKMAEEQGLDLVEISPTVDPPVCKIIDYN
                     KFLYDKKKKEKEMKAKSKVSEVKEIRFTPNTDDHDFDFKSKHAIEFLKDGNKVKAYVQ
                     FKGRAIQFQDRGQLLLLKFAERLAEVGVLENMPKMEGRRMLAMFAPKGKKKT"
     gene            complement(7897..>8064)
                     /locus_tag="PK977_04500"
     CDS             complement(7897..>8064)
                     /locus_tag="PK977_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF17400.1"
                     /translation="DTELMRVPYMLVVGEKEMSENKVAVRRQGKGDIGVKTVDDFMNE
                     IVSEIKERRAE"
     assembly_gap    8065..8083
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8084..>8573)
                     /locus_tag="PK977_04505"
     CDS             complement(<8084..>8573)
                     /locus_tag="PK977_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF17401.1"
                     /translation="PDKLGQKYVDEPELWKETEALVRDVLIKTNTPFVEVQDEAAFYG
                     PKIDVQIWSAIGREFTLATNQVDFAQGRRFKLEFTNQDNKAETPLIIHRAPLGTHERF
                     IGFLLEHYAGKFPVWLAPVQVKVLPISDKFMDYAKSVSDKLKKADIRSEVDDRNEKIG
                     KK"
     assembly_gap    8574..8717
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8718..8860)
                     /locus_tag="PK977_04510"
     CDS             complement(<8718..8860)
                     /locus_tag="PK977_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17402.1"
                     /translation="MNCPHHHKIFAAEPKSYKDLPLRLAEYGTCYRYEQSGELHGLSR
                     VRG"
     assembly_gap    8947..8981
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8982..9849)
                     /locus_tag="PK977_04515"
     CDS             complement(<8982..9849)
                     /locus_tag="PK977_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRF17403.1"
                     /translation="MINITLPDGAVRQYETGTTALDIAKSISEGLARKVLAANVNGQV
                     WDSTRPIYEDATLKLLTWNDTDGKSTFWHSSAHLMAEAVEAMFPGVKFWVGPPVENGF
                     YYDMDLGDRKMSDDDLVALEKKMNELAKQNNSYVRKEMPKEDAVKYFTEKGDEYKLDL
                     LSNLNDGEITFYTQGSFTDLCRGPHIPNTGFIKAIKLMSIAGAYWKGDEKNKMLTRVY
                     GITYPSQKELDEYLQMLEEAKKRDHRKLGKELSIFTMDDDVGPGLPLWLPNGTIIIEE
                     LEKLAKETELAAG"
     assembly_gap    10014..10038
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10039..>10380)
                     /locus_tag="PK977_04520"
     CDS             complement(<10039..>10380)
                     /locus_tag="PK977_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HRF17404.1"
                     /translation="YFFNAAEAYKKGERISPVWMAYYEKASLNPMQYELLGINAHING
                     DIWQALVKEFSLHEIRENRKIFLQFQKGLGVIYRNFHEKAKQENQRIKTLNNLSLGID
                     HFYGRLMLKRW"
     assembly_gap    10381..10399
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10724..>11788
                     /locus_tag="PK977_04525"
     CDS             10724..>11788
                     /locus_tag="PK977_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17405.1"
                     /translation="MKRILLALHITLATLFLHVSLYGQDLTGIWKGYFISDDGSYYKL
                     EFQVAQNKSNAMTGVSYSYLDVRFYGKATMTGRFLKSSSKFLIQEIKTVEVKSTLGGL
                     TCIMNYNFKYSRSGKEEFLDGTYLGMYEKKYTREEPGEWGNCGGGRVHLRKVTTSDFY
                     IEPFLRNKINNNNKPVIVNQPPIKKDTAKAITKTPVTKPPVTKNPPVSKPVDKPVTKV
                     TNPVTKTTDSAKTKPNPVVTPTPAVIKNRSNELVKTLTVNDPNVIVKLYDNGEIDGDT
                     ISVYLDKKLVLAAKRLTASPLVIKFTMEEDEEEHELTMVAENLGTIPPNTSLMIVEAG
                     EQRFDVRITSTEQKNAVVKFR"
     assembly_gap    11789..11996
                     /estimated_length=208
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12025..>12362
                     /locus_tag="PK977_04530"
     CDS             12025..>12362
                     /locus_tag="PK977_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17406.1"
                     /translation="MQLQVAGTRITGTAYHFSDSLNYVRENFEGIYNKEKNQIIIQEN
                     GIITFRIKEDCVPCVKRYELSYHKGGGNVVTEEQLRGTWTGKATDGKTVCDPGTIVLT
                     RFDKSSFKPD"
     assembly_gap    12363..12375
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12376..12760
                     /locus_tag="PK977_04535"
     CDS             <12376..12760
                     /locus_tag="PK977_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17407.1"
                     /translation="KLPATLTNRKAELVKEIKVDTGTIQIDFYDNGQIDGDTISVYVN
                     SSPVVSNKRLTTKPVSISVKIDLKKPVQELIMVGENLGSIPPNTALMIVNAGDKRYQL
                     YLTSDEQKNAMVRFIYEKPGAEKSN"
     gene            12911..13300
                     /locus_tag="PK977_04540"
     CDS             12911..13300
                     /locus_tag="PK977_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRF17408.1"
                     /translation="MSAPKLIKPTESELEILQILWNKGLATVREVHEELAKNKDVGYT
                     TTLKLMQIMNEKGIVKRDDSMRTHVYQAAVNKEKTQKHLLNKMIDSLFGGSPTQLVIQ
                     ALGDESHKASPEELEKIQALLDSLKKH"
     gene            13303..>14888
                     /locus_tag="PK977_04545"
     CDS             13303..>14888
                     /locus_tag="PK977_04545"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRF17409.1"
                     /translation="MISIQTNFFQALGIALLNSLWQLAFLWVIFQFITVFFKHGRFTL
                     KSALATSLLMSGFGWFIITFISALNTKDPGSAVLITLIPENLGEIDKGNWLLNFYPIA
                     AFIYLVLLVIPLQRLIKNYRYVQALRTYGLAKIDIDKRLFVDRVAEQLGVTKKVRLWI
                     SEFVTSPATIGYLKPVILIPAAAVTNLTPQQLESILLHELAHIRRNDYLINLVINFIK
                     AILYFNPFVKAFVRIIEKDREKSCDEMVLQFQYNSKDYAAALLTLEKDQQQKALAIAA
                     SGRTTDLINRVELILGINRQPFFPLYKLAGVAASLMVILFINTLIFSGINKNTGTLLA
                     FFTQRVADPARTISHQYPVINNNLKESAFKEKEIPVDPKAVEQGLMVPPAPPPSDYIN
                     VAFEEISIPELKKYQEDQVKKALEASKKILEKAEWEKVESNIAEALSMKEKEELKASY
                     FKELEKFDWKQWENKLKIAYNNIDWDRVNSELNMAINQIRADSLHRVYNEAAVKLDKV
                     KRQLQENQLKGIPDSDISIEA"
BASE COUNT         4280 a         3032 c         2691 g         4312 t
ORIGIN      
        1 tacttgtcac gaaaaaaaca aaaggctgca aatgactcta accagtattc attctctttg
       61 taaaacactt tgaagtcttc agatgcaaga cattccacac ccatgaaatt gtataactcc
      121 cgaagcacag agaacttata tttcatcact tcttcataat ccacaactga aagggtgttt
      181 agctcctcct gttttttctt gagaagtttc aactggcctg catattcttt ccctgcaacc
      241 ttttgcaaat tgatatagat tgggtgaagt gcaaaagcag aaatagcccc gtannnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttattcaccg gcaacaactg aattagtttt
      361 aagccagtta gacgggccca gtcaacaagg agttttatat cgttgaattc tcctacacca
      421 aaaccatttt tgctacgtag actaaataca ggaattgcta cacctgatcc cttccatgta
      481 ttattaggta attgaacaaa gccatcatgc agaactgtat acttgccttt tgatgcgtca
      541 ctgtacaata accggttgtt acctccctcg tattgaacaa actgcttctc tttcacattg
      601 taaacgccga acttataggc taaaggaaag ctggcttcag acaaatccag ctttactgtc
      661 caccaatttc cttctcttgc taaaagtgaa ggtttatcag tattccaatc tccaagctta
      721 ctgccattac cgcaaaggca aactgcttct ccttttttta acaacggtgc tttcactcta
      781 aaaatatgtg ttgcatgctt gtcggaggaa gatttgattt tagtttccgt agactttagc
      841 aacacttttt caaatggagc agtaaaaaat gcgttctcat attcaccagc atgattccaa
      901 gtatcaacaa aatgtacttc agtaatttct tttccgactg cctctccggt cctgtcatta
      961 ccccattcat tcaataattc accttcttca ttcttcagca catatttgta ttggaaactt
     1021 ttctcaaaat tctttcgttt tacatccagg cttaccgtcc agaattcatc atttaaataa
     1081 acaaggggaa tggctttggt catatcatta ttaccaagct cttcaatatt acctgtcagc
     1141 caaaggcttt ggccatactt agtatgaaat cgtaaataaa actggagttt catcgcaatc
     1201 gttcgttagt tcagtaaaga ttcgatttgt gttttaagga cacattacta cgaaagtaga
     1261 aaaaactggc aaggtttgta cttttatttg cctttttttg ccaaaatagt cggcgcaaat
     1321 ccatgaatat taattacaaa agctggaaaa tgctattcct tttctgcctg ggacttttcc
     1381 tgggcacagc tttttgcatg aaatggatgg agaaagacct gcttcaggac aaccggttgt
     1441 ttacagtcat agggcttgaa ataacctacc ctcaggaaaa agtatatacc atcctttccg
     1501 gcctagataa caaaacaagg acgatacttc aatatcacct gtactttgat tttgttttta
     1561 tgtcaggagt tttcccgggt attgctgctt tgtgtatgat ggcaagattt aagtcaaccc
     1621 atagaaatta ccgaaaaatt ctgctggcag ctgcagtttc ccaaatcgtt gcctggttat
     1681 gcgatattat tgaaaacaat ctcctgcttt catgggtagc agaccctgaa agaatcagga
     1741 tgtttgatga atttcatctg attgtttggg tcaaatggat tctggcgata ctaggattta
     1801 tcttatcgat accagtcgtt ttcaaaacga gaacccggta aaatctcatt ttttgattta
     1861 atcccacgac cttacatttg tgcaaaattt gaatccaatg gagcaaacaa aaaaatacaa
     1921 gggtctttgg tggcttgttt ttcttgcctc tacagcagca ttgctttttg ctatttattc
     1981 acactgggaa tggttaacac ttattctacc ctttcagaca acagcttttg taaaggccat
     2041 ggatataatg taattgcagc ctagaaaata tgcggcccgg ttttaatcgg gctttttttt
     2101 tgatgtggca tattacttgt tggaaaaatg cattcttcat actaaaaacg aactactatg
     2161 agaaaacttt tattgaacct tgcggcattg cttttattcg gatcttcaat caattactgt
     2221 tcagcggcag tgattccatc caccataagc agtacaaatc ccgacccggc cacagttaag
     2281 gcagccattg aaaactttaa aagccttcca cggaaagaga gaaaagaaag agtcaaactg
     2341 gcaaaaaaag aaataaaagc attcaatgcg gctaagaaag caggcaaaga agctgataca
     2401 aatacactgt tgctggttat tttggcaata ctgcttccgc cattggccgt atatcttcat
     2461 cagggtgaga taaacaacaa attctggatt agcttgctgt taacgcttct tttttggctt
     2521 ccgggtgtta tttatgccct gattgtcatt ttaggcaacg gataaagttc cccgtatttg
     2581 tacagaaagc ctgtttcata cgagacaggc tttttttgtg aggaactgtt atgtatattt
     2641 agctacaaca aaccgttaaa ccaacaacaa aatgaaactg ttttacattt cattatttgc
     2701 tttccttgta tcctgcaaat ccaataggaa gcccggtgca aataatgatc ccggctccat
     2761 caatgacaag gatagtaata ccgtttcagc gaataaaaca aagaccgaga gtactataat
     2821 acccggatca ccgctggcag aaaaaataaa caccatcctt gggtcaatga atgaaagtgc
     2881 tgcctggcat gtattaaatg ataaagaagc caggtggatc agcggacaat ttgagtattt
     2941 cattgctcct aaaagaaaag acaatcctga ttatccctat atagcatcag gtgattttga
     3001 cggagataag cggcctgatc ttgcagctgt agttactgac agtacccggg taaattacca
     3061 acttgtaatt ataagtgact tagacagcag caaaaaaata aagtattgga aagaagatat
     3121 aatggaagat gccgccattt ccacagcacc caaaggttat atagaaggaa cattaaatga
     3181 aaaagtcaag aaagtgaaat tagaatcgga agcaattcat gtagagtatt ttgaaaaagc
     3241 ttcttttatt atttactggg atggtaaatc atttaaacgt atacaacaag gagattaatt
     3301 attaatcaca aaaccaacca aaaaatgaag aaaattataa ctgtgttttc agttgttgca
     3361 cttttatcag cttgcagtgg tgcaggaagc agtggttccg acctcgccaa agaagtatgc
     3421 gattgctatc aaaaagccaa tggaatgaat gcttcagacc ctaacagggc taaggcacag
     3481 gctgattgca tggcttctca aggtattgcc tggaacaaaa taaaagacaa tcaaaaagaa
     3541 gcagacgagt ttaataaaat catcggggac tgcggaaaag aactgataaa aaagagtgtt
     3601 caataaaatg aaaagtcctg cgttgcagga cttttcattt tatattgtta tcggactatt
     3661 tcacagaatc aaccaggctt ttgaatgttt ccggattatt catggccaga tcagccagaa
     3721 ctttacgatc cagctcaatt ccttttttct gaagtttgtt gatgaactca gaataagtca
     3781 acccttctgc tcttacagca gcattaatac gggcaatcca aagggaacgg tattctcttt
     3841 tcttgagttt acggcctaca aacatgtagg tcatcccctt ttctacaacg ttcttggcta
     3901 ccgttaagac attcttacgt ttaccataga aacctttggc ttgttttaat atttttttgc
     3961 ggcgggcttt tgaagccact gcattttttg aacgaggcat attgtaatta gtttagagtt
     4021 aaaagaaaat gtacaaaatg taagcagggc ttatttcagc ctgagcagac gttttacaaa
     4081 atcaacatga gattttgcta ctgttccgtc taatcgcatg ttacgcttac gtttttttga
     4141 ttttttggtc agaatgtgac gcttaaaact cttctgatgc atgatctttc cagtagccgt
     4201 tactttaaag cgttttttgg cactggagtt tgtttttact ttaggcattt tacccagtat
     4261 ttaatattac acccttgagg cattccccac cgggggacct ttttcgaacc tcttcaggca
     4321 atctttgctc ctttggattg ttttatccgg agacaaaaca agaactaccg tttttcaaac
     4381 ggagtgcaaa gataaggttg aacagcctat tgacaaagct cctgcaaaga aaaagggaat
     4441 gcctgaaaga catccccttt cctgaagaaa atagaagcct taatctttag ggttcaatgc
     4501 gttatcaatt cgttttgcaa gatcctgcaa atgatatttt gtcataaggt ctaccgttcc
     4561 ggctgctgct gcattaatct cagccctcag agcggctaaa tgccccataa taacactctt
     4621 agcatcgctc ttatctgtgc cggaagttgt ggttatagta attcctccga tagtcgtggt
     4681 accggatgaa cttggtttta ccaaacttac caatgtattg atatatgatt tctgcaggtt
     4741 tctcctgtaa atatcaatag gtttccgggt ggctaattct gtccaaatac cctttttgag
     4801 atcgccgagg agttctgtca tttgataggc attgtttccc agtgttgctt ctgcttccag
     4861 taatttggta aacgttctgt ttgaaagcag ccggccgaga atattatcct gtacaccacc
     4921 gatagtggtt aatcctgaca gacctgtttt accgaaaatg tcctgattta ttaaccatgt
     4981 tggagtggta aatagctgtt tattcaaaaa atctactgcc tctttttgtt tggcttctgc
     5041 aacgatttca tatactggcc cctgctcttc tactcttttg ggtgtttcca taattccacc
     5101 tacatattta gccacatgcc ccatatacct gctgtattgt ccaacaacct ggttataaat
     5161 ttttgataag cctgaaaagt cttcatttgg catttttgtc cactccatca ggttgggtac
     5221 aattctctgc aaattcttta ccccgtaaaa actgcccttc atggcatcat ctcccacttg
     5281 ctcactttgg ctgcgtgggt catcgggatt ggtttccgtc ccaaaccaaa gacgcttgtc
     5341 ctttagcttt tcaataaccc aattgttaag gtgcgtcttt tcggcatcgg ctgatttata
     5401 ttggaggaat tgtttgtatc cccattcaat ggcccattta tcgtaatcgc caatcctggg
     5461 aaaaataccg gaccggtcaa tcttatcttc aggttgagcg acataattga aacgagcata
     5521 atccataata gatggtgtat ggccattagc ctcaacccat gccttattgc gaagattttc
     5581 aaccggtacg gatgaactgg aaccataatt atggcgaaga cccaacgtat ggcctacctc
     5641 atgtgaagaa acaaaacgaa taagctgtcc cattaannnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc atcacattat
     5761 gataccagtt gatgtggctt tccattattt caccactacg tggatcggaa atacttggcc
     5821 cgctggcatt cggaatatct gagggcttgt atacaatggc tgaattgcgg gcatcatcaa
     5881 gactccatgt gctgtcttct tcttttgtag gagccatttt accaataata gcatttttga
     5941 agcctgcctt ttcaaatgca gtttgccagt cgttcactcc ctgtacaagg tatggtatcc
     6001 atttcttagg tgtggcaggg tctatgtaaa aaattattgg cttcttgggt tctaccagtt
     6061 ctcctctctt atacttttca atgtcctcgt cttttggttc cagtctccac ctctttacca
     6121 tactgataga tttcacacct tgtggatttg catcgaaatc tgtataacca actgcaaaat
     6181 aacctaccct tggatcataa taacggggtt gcataggcac tttgggtaac agcactaaag
     6241 aactgttcag ttccatcgta aggtttcctg cagccgttgc acttccaaag ccaccggtag
     6301 tggatggagc ggcagaacgg gcatatgttt ttactgccct tatttcaata ttgatcggga
     6361 atgaccttac attgacaata taggatttat cttgctgaag agccgcaagc ctgaggcttg
     6421 attttaatcc cccgttaaaa tgaagtacat cattatcgcc actgatataa tctgtaatat
     6481 caataacagc cccggaagaa tccttaccca atgatttaat atcaaatgat gcagcaattg
     6541 gctgaatatt tgacttgttc actgccgtaa acatatcaga agtagagtct tttgtatatt
     6601 ctgcaaaaga gatattgcgg agaaaaatct tgttgttagg ccctttttca aagcgaataa
     6661 cattctgccc gatctgatca ccgccataac caaagaaact accagagctg cgcattcctg
     6721 cagctgcttt ggaaatgcgg tttaccacca gtatttctct cccgaaaaga gaatcaggga
     6781 tctcaaaaaa gtatttatca tctactttgt gaacccaaaa cagccctgca tcagacattg
     6841 ccctggatgt gataacttct ttgtaaggct taagaccgga attatttgaa ccaggtcttc
     6901 ttgtagaatc ggggcgcaag ccaggaggct gagcataaac aataactgtc acaaaagaaa
     6961 aagtcagcaa gcataaaatt ctcttagtgg aggatatcat aattttcagg tatttaaagc
     7021 tctcaagata cgtattgaaa tggtataagc gtctttgcag gttattttta taccaacggt
     7081 ttaaaaaagg tatagcatca catcacacat tacaaaaaag cctgtcttgt aaaacaaaga
     7141 caggcttacg agacgaaatt cttaaacatc aggttttctt cttgcctttc ggtgcaaaca
     7201 tggccaacat tctgcgacct tccatctttg gcatattttc aagcactcca acttctgcta
     7261 acctttctgc aaacttcagc agcaacaact gaccccgatc ctgaaattga atggcccttc
     7321 ctttgaattg aacataggct tttactttat tgccatcctt taaaaactct atagcatgtt
     7381 ttgatttaaa atcaaaatca tgatcatcag tgttgggggt aaacctaatt tctttcactt
     7441 cggagacttt gctttttgct ttcatctcct tttccttctt cttcttatcg taaaggaact
     7501 tgttgtaatc gatgatctta cagacaggag gatcaacggt aggagaaatt tctacaaggt
     7561 ccaatccttg ctcttccgcc attttaagcg ccacctgggt atcatagata cctacttcca
     7621 cattttcgcc tacaagtctg acttgcggca cacgaataaa atgattgata cgatgttcag
     7681 cttctttacg gggcataaaa cgacccctgt taaaacctcc tttgttgaat ccgccacctc
     7741 cgggcttaaa acctccgccc ccagatggcc tgttgaaccc gcctccgggt ttttgtggta
     7801 ctgccattta attattttag tgatgttttg ctcctgcccg gcaaaacgct tttttgttat
     7861 tagacctcct gcaggaagag gtttttaaga tacaatttat tctgctcttc gctctttgat
     7921 ttctgaaact atttcattca taaaatcatc gacagttttt actccaatat ctcccttacc
     7981 ctgccttctt actgccacct tattttcgct catttccttt tctcccacta ccagcatata
     8041 aggcaccctc atcaattcag tatcnnnnnn nnnnnnnnnn nnnatttttt taccaatctt
     8101 ttcattcctg tcgtctactt cactacgaat atcagctttt ttcaacttat ctgaaacaga
     8161 tttggcatag tccataaact tatcactgat aggcagcact tttacctgta ccggcgccag
     8221 ccaaacagga aactttcctg cataatgttc taacaggaat ccaatgaatc gctcatgtgt
     8281 tcctaaaggt gccctgtgaa tgatcagcgg tgtttcagct ttattgtcct ggttagtgaa
     8341 ctccaatttg aaacgacgtc cctgtgcaaa atccacctgg ttggtagcaa gggtaaactc
     8401 ccggccaata gcactccata tctgcacatc gatctttggg ccatagaatg ctgcctcatc
     8461 ctgtacttct acaaaaggag tgttggtttt gatcaatacg tctcttacga gtgcctccgt
     8521 ttctttccat aactctggtt catccacata tttctggccc aacttatccg gtgnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnaat ccacgcactc gcgacaagcc gtgcaactca ccactttgct
     8761 cataacggta gcaggttcca tactcggcca ggcgaagggg caggtctttg taactcttag
     8821 gctccgcggc aaaaatcttg tgatgatgcg gacagttcat cgctttcagg tagtacttct
     8881 gcccttccag ttccatgggt ggatacatac tgtcctggta atagggtaaa tgtccactgg
     8941 tgagatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natccggcag ccagttctgt
     9001 ttccttggcc aacttttcca attcttcaat aattattgtc ccgtttggca gccacaaagg
     9061 gagaccaggg cctacatcat catccattgt gaagatgctt aattcttttc ctagcttacg
     9121 gtgatctctt tttttagcct cctcaagcat ttgcaggtat tcatctaact ctttttgaga
     9181 gggataagta ataccatata ctcgggtcag cattttattc ttctcatcac ctttccagta
     9241 ggcgcctgca atgctcatca gtttaatggc tttgatgaag cctgtattag gaatgtgcgg
     9301 gcccctgcac aggtctgtaa aactaccttg tgtgtaaaaa gtaatctcgc catcattcag
     9361 gttgctgagc aaatccaatt tgtactcatc tcccttttct gtaaaatatt ttacagcatc
     9421 ttcctttggc atttcttttc ttacatagct gttattctgc tttgccagtt cgttcatctt
     9481 tttctccagt gccaccagat catcatcgct catttttctg tcacctaaat ccatgtcgta
     9541 ataaaaaccg ttttccacag gaggaccaac ccaaaatttc accccgggga acattgcttc
     9601 aacagcttct gccatgaggt gagcggaaga atgccaaaaa gtgctttttc cgtctgtatc
     9661 attccatgtc agcaatttca atgtagcatc ttcatatata ggtctcgttg aatcccacac
     9721 ctgcccgttt acattggcgg ccagtacttt tctggccaat ccttcagaaa ttgactttgc
     9781 aatgtcaagg gctgttgtac cagtctcgta ttgcctcaca gctccatctg gtaaagtaat
     9841 gttgatcatg taataaaatc ctcgttttta agagattgca aaagtagcaa aatcagccta
     9901 aagatggtga agaataaggc ggttaatacg gttcattttc ttatcgagcc ttatcaattc
     9961 agctttcatc ttttcggact tatcgaaata ccattctgcc agccggattt gtcnnnnnnn
    10021 nnnnnnnnnn nnnnnnnngc caacgtttta gcattaacct gccatagaag tgatctattc
    10081 ccagggagag gttgttcagc gtttttattc gctggttttc ctgtttagct ttttcatgaa
    10141 aattccggta aataacccct aatccctttt gaaattgtaa gaaaattttt cggttttccc
    10201 gaatctcatg taaagaaaac tctttcacaa gggcttgcca tatatctcca ttaatatggg
    10261 cgtttattcc caacaattca tattgcatgg gattcaacga tgccttttca taataagcca
    10321 tccagactgg tgaaattctc tccccctttt tataggcctc tgccgcattg aaaaagtagc
    10381 nnnnnnnnnn nnnnnnnnna agaatgttcc agtctttgaa tgaatgacct tttcttttga
    10441 tccagattca gaaaaaaaaa tattgcctca acggtggttt cgcggtatag tttggcgaaa
    10501 tgactggaaa tagatgacga tttggcaatg gaatccagcc tttttaatag actgatttcc
    10561 gaatcgctta tttgccctga gccgtaaaag ggaattaaaa aataaattgc aaaagctgcc
    10621 aaccttattc gcataacccg gataatcaaa gggttaaaat taacaaactc ttgccggata
    10681 caggtttttc cgtacaaatc catttgttag ttttgccctc attatgaagc gaatattgct
    10741 ggccttacat attaccctgg caactttgtt tcttcatgta tccctgtatg gacaggatct
    10801 taccggtatc tggaagggtt attttatttc tgacgatgga agctattaca aactcgagtt
    10861 ccaggtagct caaaacaaat ccaacgccat gacaggtgtt tcttattcgt atctcgatgt
    10921 ccgtttctat ggaaaggcta ccatgacggg taggtttctc aaatcatctt caaaattcct
    10981 gatccaggaa ataaagaccg tggaagtgaa aagcacttta ggcggcctca cttgcatcat
    11041 gaactataat tttaaatatt ccaggtcggg taaagaagaa tttttagatg gaacctacct
    11101 gggaatgtat gagaaaaaat atacccggga agagcccggc gagtggggaa attgtggcgg
    11161 tggaagagtt caccttagaa aagtaaccac ttccgatttt tatattgagc ccttccttag
    11221 aaataaaatt aataataaca acaagcccgt aattgtaaac caacctccca taaaaaagga
    11281 tacggccaaa gctataacaa agacacctgt tacaaaacct ccggtaacaa aaaacccccc
    11341 ggtttcaaaa ccagtggaca aacctgttac caaagtaacc aaccctgtaa ctaagacgac
    11401 ggattctgcc aaaactaaac caaatcctgt tgttactcca accccggcag taataaaaaa
    11461 cagatcgaat gagttagtta agaccctaac tgtaaatgac cccaatgtaa ttgtgaagct
    11521 atatgacaat ggagaaattg acggagatac tatttctgtt tatttagata aaaaattggt
    11581 actggcagca aaaagattga ctgcctctcc cctggtgata aaatttacta tggaagaaga
    11641 tgaagaggaa catgaattaa ccatggttgc cgaaaacctc ggaactattc ctcccaacac
    11701 ttcgctgatg attgttgagg caggagagca acgttttgat gtacgtatta catcaacaga
    11761 acagaaaaac gccgtggtga aattcaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgga
    12001 tgcttccctg tttataatat tgaattgcag ttacaggttg ccggcacccg aattaccggt
    12061 accgcctatc acttttcaga ttcgctaaat tatgtaagag agaattttga aggaatatat
    12121 aacaaggaaa agaaccagat aatcatacag gaaaatggca ttatcacatt tcgtattaaa
    12181 gaagactgtg ttccctgcgt aaagagatat gaattaagtt atcacaaagg aggcggcaat
    12241 gttgtcaccg aagaacaact gaggggtaca tggacaggaa aagccacaga cggcaaaact
    12301 gtatgcgacc ccggtaccat tgtattgaca agatttgata aatcctcttt taaaccagac
    12361 ttnnnnnnnn nnnnntaagt tgccagcaac actcaccaac cgaaaagcgg agttggtaaa
    12421 agaaattaaa gtggatacag gtaccataca aattgacttt tacgataacg gccaaataga
    12481 tggggacacc atctcagtat atgtaaacag ttcgccagtt gtttcaaaca aaaggttaac
    12541 gaccaagcca gtctccattt ccgtaaaaat tgacctgaaa aaaccagtac aggaactgat
    12601 catggtgggg gaaaatcttg gttccattcc acccaataca gctttaatga ttgtaaatgc
    12661 cggtgataaa agataccagc tttatcttac ttctgacgaa caaaagaatg ccatggtcag
    12721 gttcatttac gaaaagcccg gtgccgaaaa aagcaattag aaaagttgat acatttcatt
    12781 gaattagctg gtaacttatt tacaagattc tgcgtctcac tagttagcag gtgttaaaaa
    12841 aggctaaaaa aagttatagc cataacttcg tacgaaaata atcgtatatt cggccataaa
    12901 attgagtact atgtcagcac ctaaactgat caaacccaca gaaagcgaac tggaaatcct
    12961 tcaaatcctt tggaataaag gattggcaac cgtaagagag gttcatgagg aactggcaaa
    13021 aaacaaagat gtgggatata ccactaccct taaactgatg caaataatga atgaaaaagg
    13081 gattgtaaaa agggatgatt cgatgaggac ccatgtttac caggcagctg ttaataaaga
    13141 aaaaactcaa aagcatttgc tgaataaaat gattgatagc ctttttggag gctcccctac
    13201 gcaactggtt atacaagcat tgggagatga aagccataag gccagtccgg aagaattgga
    13261 aaaaatacaa gccctgttag acagtttgaa aaaacactaa acatgatcag catccagact
    13321 aacttttttc aggcacttgg cattgccttg ttaaacagcc tctggcagtt agccttttta
    13381 tgggtcatct ttcaatttat cactgttttc ttcaaacatg gcaggttcac tctaaagtcg
    13441 gcccttgcca cctctttgtt gatgtccggc tttggctggt ttataatcac tttcattagt
    13501 gcacttaata caaaagatcc tggcagcgct gttttaatca cacttattcc ggaaaacctt
    13561 ggtgagatag ataaaggcaa ttggttgtta aacttttatc ctatcgccgc ttttatctac
    13621 ctggttcttc tagtaattcc attgcagcgg ttgataaaga attatcgtta cgtacaggct
    13681 ttaagaacat atggccttgc caaaattgat atagacaaga gactgtttgt tgaccgtgtg
    13741 gctgagcaat taggagtaac taaaaaagta agattgtgga tttctgaatt tgtaacctct
    13801 cctgccacca ttggttacct gaagccggtg atacttattc cggcagccgc ggtcaccaat
    13861 cttacacccc agcaactgga gtctattctt ttacatgaac tggcacatat ccgccgaaac
    13921 gattatctca ttaatcttgt tataaatttc ataaaggcaa tcctttactt taatcctttt
    13981 gtaaaggcct ttgtccgtat catcgaaaaa gatagagaaa aaagctgcga tgaaatggta
    14041 ttgcagtttc aatacaattc gaaagattat gctgcagctc ttttgacttt ggaaaaggat
    14101 caacagcaaa aagcgctggc tatagcggct tctggaagaa ctactgatct tattaacagg
    14161 gtggaattaa tcctgggcat taacaggcaa ccatttttcc ctttatataa acttgccggt
    14221 gtagctgcaa gtttaatggt aatcttattt attaatacac ttattttttc aggtataaat
    14281 aaaaataccg gaacgttatt agcgttcttt actcagcggg tagcagaccc tgcccgaacc
    14341 atcagccacc agtatcccgt tataaataat aatctaaaag aatcagcctt taaagaaaaa
    14401 gaaatacctg ttgatcctaa agcagtagaa caaggtttaa tggtaccacc tgccccgcct
    14461 ccatcggatt atatcaatgt tgcttttgaa gaaatctcaa ttccggaatt aaagaaatac
    14521 caggaagacc aggtaaaaaa agcccttgaa gcttcaaaga aaatattgga aaaagcagaa
    14581 tgggaaaaag tagaaagtaa tattgctgaa gcactcagca tgaaagaaaa ggaagagcta
    14641 aaggccagct atttcaaaga actggaaaaa ttcgactgga aacagtggga aaacaaatta
    14701 aaaattgctt ataacaatat tgactgggac agggtgaaca gcgagttgaa tatggcaatt
    14761 aatcaaatca gggcagacag ccttcaccgg gtatataatg aagctgccgt aaaacttgat
    14821 aaagtaaaaa gacagttaca ggaaaaccag ttaaaaggaa ttccggattc tgatatttct
    14881 atcgaagc
//
LOCUS       DAORLS010000036        14768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2180_length_14768_cov_1.944199, whole genome shotgun sequence.
ACCESSION   DAORLS010000036 DAORLS010000000
VERSION     DAORLS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2180_length_14768_cov_1.944199"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK977_04550"
     CDS             <1..180
                     /locus_tag="PK977_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17410.1"
                     /translation="YTVQFTNQSGQVVLQKEIWIDSAAKVLNIDAPVTTVGNYFITLV
                     SKKTAKKFTEKIIIQ"
     assembly_gap    282..310
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..1142)
                     /locus_tag="PK977_04555"
     CDS             complement(<311..1142)
                     /locus_tag="PK977_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HRF17411.1"
                     /translation="MWEPYKKGFKAYLELEKSLSPNSVEAYLRDLEHLTAYLQEKGIL
                     LNPSAIKLKDLQQFVKWIAELGMTATSQARIISGLRAFYKYCALEDISKTDPTILLEA
                     PKLKRALPDVLSFDEIESIIAQIDLSKPEGGRNKAILETMYSCGLRVSEVVNLKISQL
                     YLDLGFIRVLGKGDKERLIPIGSSAIKYITIYKNDIRVHLTPKKGNEDVLFLNNRGSK
                     LSRVMIFYIIKDLALKAGIKKTVSPHTFRHSFATHLVEGGADLRAVQEMLGHASITTT
                     E"
     gene            complement(1142..1801)
                     /locus_tag="PK977_04560"
     CDS             complement(1142..1801)
                     /locus_tag="PK977_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17412.1"
                     /translation="MKLLVAPAIAVVLILSITGCKSSSADGPALFCDTTCLKDTIKFN
                     GDFALKPYVYISAKDCRADTLIWSYKGMGINRKTGFTYLLNNSVSINKDNIRCVFNDT
                     SYAWLLFNDCATGRGYQLRLPFNKTADIGRKSSGINNIDKKFSISDNLVVNTDRGNIY
                     IEDMITGKKAMMTFGQKLDIDYDAIHEYIDSVNITNSRIWVKILVDNKWVEKEKNITL
                     E"
     gene            1907..2419
                     /locus_tag="PK977_04565"
     CDS             1907..2419
                     /locus_tag="PK977_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRF17413.1"
                     /translation="MSKIPPVNLRSFKRKAQYAKTKMRRFLNKLEKDRPRHLDKKIEV
                     MEKEVWKETDCLSCANCCKTMTPTYNKKDLERISAHLKMTVDEFKKKWLKQERGGDRD
                     WMNKHTPCQFLNPDDNMCSIYEVRPDDCAGFPHLSKKFKDFVHIHKQNVEYCPATYKL
                     VEKMMEGIAP"
     gene            complement(2430..2786)
                     /locus_tag="PK977_04570"
     CDS             complement(2430..2786)
                     /locus_tag="PK977_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRF17414.1"
                     /translation="MASHNELGKEGEEKAAQWLVTKGFTLLHRNWRHSYHEIDIIATK
                     GKFLHFIEVKARKASSLGNPEDSVTKKKFKNLQKAANEFLFLNPGYNWIQYDILSISF
                     QKNGNAEYFLLEDVFL"
     gene            2825..3997
                     /gene="manA"
                     /locus_tag="PK977_04575"
     CDS             2825..3997
                     /gene="manA"
                     /locus_tag="PK977_04575"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HRF17415.1"
                     /translation="MSGIYRLHGVVKHYDWGGYSFIPALLKAENKPKKPFAEYWMGIH
                     PLGMAGIDTGGSSPTDLAFLAPDLSYLFKILDVRDMLSIQVHPSKEAAETEFKRENAE
                     GIPIDSPQRCYKDPNHKPELMVALGDFWLLHGFKPTEELTDTLLNIAELRELLPVFNN
                     SGYPGLFKHVMEITQEEVNRILKPLIDSLNKIYESGEMEKSSPDYWAVKAHKTFSSDG
                     NIDRGIFSIYFFNLIHLKKGEGIFQAAGVPHAYLEGQNVEIMANSDNVLRGGLTTKQI
                     NVKELMKHVKCEATYPQILSPEADNEGERKYETPVDDFRLSVFELEAGDIVSFYPQEV
                     ETVLLTEGITEIDNENDTIRLQAGSPAAVVFPGDKVYLAAAAKSFVFRASGRIHNR"
     gene            4058..4627
                     /locus_tag="PK977_04580"
     CDS             4058..4627
                     /locus_tag="PK977_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17416.1"
                     /translation="MKFNGRMLVFTIALIALATACKFFFGPDLDWSGFSPVIAIALFA
                     GFIIKHKDMSFIMPLLALFISDAIIHLLYKTGNFEYAGFYGGQWKNYLLILSATLLGW
                     IFKGNSYIGLVAGGIIAPTVYFLLSNFMVWQATTEAVYAKSFNGLMTCYEAGLPFYRN
                     SLAAMLVFLPLILLSYNYVTGKKAVLTTA"
     gene            complement(4703..5173)
                     /gene="rnhA"
                     /locus_tag="PK977_04585"
     CDS             complement(4703..5173)
                     /gene="rnhA"
                     /locus_tag="PK977_04585"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRF17417.1"
                     /translation="MSHNVTIYTDGSSRGNPGPGGYGVILMSGNHKKELSQGYKLTTN
                     NRMELMAVIAGLRALKKEDMHVTIYSDSQYVVKAWEEGWLKNWIKTNFKGGKKNPDLW
                     KDFYELSQKHHIRFKWVKGHADNPYNNRCDELATEAADGHHHTLLTDDGYENKS"
     gene            complement(5176..>6336)
                     /locus_tag="PK977_04590"
     CDS             complement(5176..>6336)
                     /locus_tag="PK977_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF17418.1"
                     /translation="GRIFRNNARMSGMGITQIAAVMGPCVAGGAYLPIMSDETLMVEG
                     NGSIYLAGPYLVKAAIGEDIDNETLGGATTHTEISGIADYKFKTEQECMDHIKRMMSM
                     LGEKEKAGFDRITPKLPKKNPEELYGIFPSDGKPYDVLDIIERIVDDSNFEQFKADYG
                     KTIVCGYGRIDGWAVGVVANQRKIVKSRKGEMQMGGVIYNDSADKAARFILNCNQKKI
                     PLVFLQDVTGFMVGSRSEHAGIIKDGAKMVNAVANSVVPKITIVTGNSYGAGNYAMCG
                     KAYDPRFIYAWPTAKIAVMSGDSAAKTLLQIEVASKKAKGEKLSEEEEKKMFDKIKQR
                     YDKQTSPYYAAARLWVDAIIDPTETRKMISEGINAANHNKEINELRTGVFQV"
     assembly_gap    6337..6447
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6448..6790)
                     /locus_tag="PK977_04595"
     CDS             complement(<6448..6790)
                     /locus_tag="PK977_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF17419.1"
                     /translation="MNLEFNRNEDQLKQSLSDMRRKLENIYEGGGKKSIEKQREKNKL
                     TPRERIDYLLDPDKPFIEIGAFAGYEMYEEQGGCPAGGTVAGVGYVSGRQCVIVANDQ
                     TVKAGAWFPITG"
     gene            complement(6862..>7446)
                     /locus_tag="PK977_04600"
     CDS             complement(6862..>7446)
                     /locus_tag="PK977_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17420.1"
                     /translation="YRRLGMAVLKDKPEKTFIAGHNGGGKGFTIDVYFDYYKGDIVVW
                     CSNQYGAAYELTGRAFAILNNKKYNKPIHADRIRLADWLLKQPTNNIPVITDSVLSQL
                     NIEAANEFLFFSVFDNLTEVHEYRAAKAAISTGRERFPSSAYMWIKSGENALNLNEGV
                     EAKRYFEKAFLLAEENKDSALITVIKQKIASCPK"
     assembly_gap    7447..7469
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7470..8372)
                     /locus_tag="PK977_04605"
     CDS             complement(<7470..8372)
                     /locus_tag="PK977_04605"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF17421.1"
                     /translation="MEANNFFNQKNIQQFNPAYLCIVVLTFFFTVCCSAQLQQSRFDS
                     LIKQYQQLNWNGVLLIGNKDKITYSATLGFSNRETRAPMQLNTAFKTESVGKMFTAIR
                     ILQLIKAGKLSLSSSVAKLLPDWKIPNADKMTIQHLLTHTSGLSSQWEHPAYEFGKIY
                     TRNDIKKFIEEAPVIFTTPGERSYYSNSGYVLLEEIITNTDNMSFEESIKKNVFYVAG
                     MQQTRSLNDSVLPSNAARPYYQITEKLFSPDITKYGDGKASGAGGWISTAEDLFLFAQ
                     AYLNEKLLPRKWMEIQLTNNHTIDS"
     gene            8524..8874
                     /locus_tag="PK977_04610"
     CDS             8524..8874
                     /locus_tag="PK977_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17422.1"
                     /translation="MTPAARSVCYFGYYLYVVGLSLITVPNLFLRTMQLPETNEVWIR
                     IVGVLVFCLGFYYHRSSVYNNVPLLKLTIPARIFAALCFFSFVLLNLAPPMLAVFGAV
                     DLMAAVWTFIAFRN"
     assembly_gap    9309..9408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9409..10935
                     /locus_tag="PK977_04615"
     CDS             <9409..10935
                     /locus_tag="PK977_04615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF17423.1"
                     /translation="KQKVKSRTIKSSDFESICELQLTCFPGMKPWSRQQFDSILSNFP
                     QGQFCVEYDGKLVASSCSLIINRSQYPDTASWNELTNTGYITNHDPEGDTLYGMEIMV
                     HPEYRLMKLARRLYEARKNLVRELNLKTILIGGRIPGYHLHSGSMDVEEYVQHVIDKK
                     IYDAVLTAQLSNGFVLKRLLPGYLPNDKESCGYGTFLEWSNLMYTDIKKPVKPDPYVR
                     ICCIQYQLRMINSFEEFAESCEYFVDVASDYRSDFVVFPEMLTMQLLTFLPQKPPAEA
                     VRQLDQFTEQYIEMFSKLAIRYNVNIIGGSHFIIENGDLYNVSYLFKRDGTWARQFKI
                     HITPHEKKWWGVKPGDEMKVFNTDCGKIAIAICYDIEFPEIVRVAASKGAGIIFVPFN
                     TDVRRSYLRVRYCAQARAIENQVYVVITGAVGNLPRVENLDIHYAQSAILTPSDIEFH
                     REGLAAEAPANVETAIVQELDINLLKRNREFGSVQTWADRRSDLYHVSFKEGDKQLRI
                     "
     gene            10971..11660
                     /locus_tag="PK977_04620"
     CDS             10971..11660
                     /locus_tag="PK977_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17424.1"
                     /translation="MTGIHILIAEDEKKIADALCQGLEENGFTAAVAYDGAIALKMFR
                     NIPFQLVIADINLPGLNGYELCHAIRAANASVPVVMLTAMSALEDKIEGFDAGADDYI
                     VKPFDFRELLVRIRALLKRTHTTVATGTLLQADDLVMNLDSKQVTRKGQPIQLTAKEF
                     QLLEYFLRNKNRVVSRADIALQVWDIDFDTRTNVIDVYVNYLRKKIDKGFDQPLIYTQ
                     VGMGYVLKDKS"
     gene            11660..>12896
                     /locus_tag="PK977_04625"
     CDS             11660..>12896
                     /locus_tag="PK977_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF17425.1"
                     /translation="MKLVYKINLLFAAIVSAIVLVIAFLIYTISRQTLENDYRQRLKT
                     RAARTAQLYTFFRSDTTNLLKSLDANAPPALFNKNINIYDDNRNILYEYHDEGTQRLS
                     PDSKRFAEAAESGEIFYSVNGKDVCLYYHKDPVMNLTVLVAADNLAGKDYIRNLKNIF
                     LLFFPAAVIIALAAGYLFSRSIIRPIKETIRDVKLITSQNLSHRLFEGNKKDEFSQLN
                     ATFNDLLNRLEESFAIQRRFISNASHELSTPLTSVSSQIEVSLLQDRSPEEYRHVLRS
                     VLDDVRELHQLTHNLLEIAKAGTDGGITLDKVRIDEVLIKAHSDLLRQNKGYRAELFF
                     DELPEDENECLVFGNYHLLYSAFKNLLENGCKYSPDASVKVYLSFRTQEVMITFTNNS
                     DMLTNEEIGKLFEPFYRGSN"
     assembly_gap    12897..12990
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13138..13995
                     /locus_tag="PK977_04630"
     CDS             13138..13995
                     /locus_tag="PK977_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF17426.1"
                     /translation="MKKLFTKILVPVNFNRNTAMAMQKAVQVANEFHCDVHVLHVQTP
                     LTILPFLYEGFFATNVYESHARTAAGKLQQLREIYGKKLNDGLLMTISQELGDWQTML
                     KKTIISEHTDLVLIPKHPKRFFGALIQKININKLIQQTKCPVLTVTRRFNIHHMQNIV
                     VPVGDTLPIRKLTMATYLARQSNGYVHLMGSSGKSKREEKQSSWCVTRAYQLLRDYTQ
                     VNIHCSIGADNNRMDGMLAVARNVKADLIVVNSGKETQPAGWLNRLLGKYVYKESNIP
                     VLTVAGTNN"
     gene            14016..14414
                     /locus_tag="PK977_04635"
     CDS             14016..14414
                     /locus_tag="PK977_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17427.1"
                     /translation="MKKITGTLIITFMFSTVLVAQDAAEVEQLLDNERYESAEAILEK
                     KLNTDGVMPDISYLLVKTYLEQDKVEEVKDFISKYKLSDSPESDPLDRITYARYLLQK
                     GDKAGADAIFNSILENKKNRKNHCSWLWPK"
     gene            14396..>14768
                     /locus_tag="PK977_04640"
     CDS             14396..>14768
                     /locus_tag="PK977_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF17428.1"
                     /translation="MAMAEVNISEPKGDASLALEWLKMAADKDDDNPSIFILEGLAYR
                     KLSDASKAYLAYQQALKKDKNNVKAHYLLGKIFTAQKNSEVYLEHFMKAYEVDSTYAP
                     VLEALYDHYYNVDVRIARKFLE"
BASE COUNT         4168 a         2794 c         3069 g         4380 t
ORIGIN      
        1 tatacggtgc agtttaccaa tcaatcaggg caagtagttt tacaaaaaga aatttggatt
       61 gattcagcag caaaagtgtt gaatatagat gccccggtta cgacagtagg taattatttc
      121 ataacactag tgagcaagaa gacagctaaa aaatttacag aaaagataat tattcagtaa
      181 gcagtttgca ggttatcata gaagtccacc ctaaggcccc ggtttatccg gggctttttt
      241 atttaaaacc gggatggaac tgttccaatg ttttgcggag gnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn tttcagtggt ggtgatgctg gcatggccca gcatttcctg tacggcccta
      361 aggtcggctc cgccctctac caaatgtgta gcaaaggaat ggcggaaggt atggggagat
      421 acagttttct ttattcccgc ttttaaagcc aggtctttga tgatgtaaaa gatcatcacc
      481 cgggacagtt tgcttccccg gttattcagg aaaagcacat cttcatttcc tttttttgga
      541 gtaagatgaa cccggatatc atttttgtaa atggtaatgt attttatagc actgctgccg
      601 atagggatca gtctttcttt atcacctttt cccagcacac ggatgaaacc cagatcgagg
      661 taaagctgag agatttttaa attaactacc tcactcacac gaagcccgca actgtacatt
      721 gtttccagta ttgctttatt gcgaccgcct tcaggcttac tcaaatcaat ttgtgcaatg
      781 atgctttcta tttcatcaaa gctcaagaca tccggcaatg ctcttttcag ctttggggct
      841 tccagtaaaa tggtaggatc tgtttttgaa atgtcctcca gcgcacaata tttatagaaa
      901 gccctgagcc cggaaattat acgggcctgg gaagtagctg tcataccgag ttcagcaatc
      961 cactttacga actgctgcag gtcttttaat ttgattgcgg aaggatttag cagtatgcct
     1021 ttttcctgca gataagcagt caggtgttca aggtcacgca gataggcttc tactgaattc
     1081 ggggaaagtg atttttccag ttccaggtat gctttaaatc cttttttata gggttcccac
     1141 attattcaag ggtaatattc ttttctttct ccacccattt attatctacc aaaatcttga
     1201 cccatatgcg gctattggtg atattgaccg aatcaatata ttcatgaatg gcatcgtagt
     1261 ctatatccag tttttgacca aaggtcatca tggccttttt ccctgttatc atgtcttcaa
     1321 tataaatgtt accgcggtcg gtatttacaa cgagattgtc ggagatagaa aatttcttgt
     1381 caatattgtt gatgccactg cttttacggc ctatgtctgc cgttttattg aaaggaagac
     1441 gcaactggta acctctgccc gttgcgcaat cattaaacag caaccatgca taggaggtgt
     1501 cgttaaatac acaacggata ttgtctttat tgatagatac agagttattg agcaggtagg
     1561 tgaacccggt ttttcggttg atgcccatac ctttatagct ccagatcaat gtatctgcac
     1621 gacaatcttt ggctgatata tacacatacg gtttcaatgc aaaatcaccg ttaaacttga
     1681 tagtgtcttt caggcaggtt gtgtcacaaa aaagagccgg tccatcggct gaagaagact
     1741 tacacccggt aattgacagg atcagcacaa cagcgatagc aggggcaacc agtagtttca
     1801 tcattgagtg gtttgattga tagcaaaaat aactattgcg cttttagtga ttaaaaattc
     1861 tttttagcag ttggccgaaa tagtttcgga tcattatctt cggcaaatga gtaagatacc
     1921 acctgtaaac cttcgttcat tcaaacgaaa agcacaatat gcaaaaacaa aaatgcgccg
     1981 ttttctaaat aaattggaaa aagacaggcc caggcatttg gacaaaaaaa ttgaagtaat
     2041 ggaaaaggag gtgtggaaag aaacagattg cctgagctgt gccaactgtt gtaaaacaat
     2101 gacacctact tacaacaaaa aagacctgga aagaatttct gcacacctga aaatgacagt
     2161 agatgaattc aagaaaaaat ggctgaaaca ggagcggggc ggtgaccgtg actggatgaa
     2221 taagcatact ccctgccagt tcttaaaccc ggatgacaat atgtgcagta tttatgaagt
     2281 acgaccggat gattgtgcag gatttcccca tttgtcaaaa aagtttaaag attttgttca
     2341 tattcacaag cagaatgtgg aatattgccc ggctacctat aagctggtgg agaaaatgat
     2401 ggagggaatc gcaccataaa acctactatt tatagaaaaa catcttccag taaaaaatat
     2461 tcagcatttc catttttttg aaagctgatg gaaagaatat catattgaat ccaattatag
     2521 cccgggttta gaaaaagaaa ctcattggct gctttttgca ggtttttgaa tttctttttt
     2581 gtgacactgt cttccggatt gcctaacgat gatgcttttc ttgcttttac ttcaataaag
     2641 tgcagaaatt ttcctttggt ggctatgata tcaatttcat ggtaagaatg acgccagttg
     2701 cggtgcagca gggtaaaacc tttcgtgacc agccattgtg ctgccttttc ttcaccttct
     2761 tttcctaact cattgtgcga agccatgtac tatctttact acaagataaa aaagaaaaca
     2821 aattatgtcg ggcatttacc ggttgcatgg cgtcgttaaa cattatgatt ggggaggcta
     2881 ttcatttata cctgcattat tgaaagctga gaacaagccc aaaaagccct ttgctgaata
     2941 ttggatgggg atacatcctt tgggaatggc cggaattgat acggggggca gttcacccac
     3001 tgatcttgct tttcttgctc cggacttgtc ttatcttttt aaaatattgg acgtaaggga
     3061 tatgctttct atccaggtgc atccttccaa agaggctgcg gaaactgaat ttaaaaggga
     3121 aaatgcagaa ggtataccga ttgattcacc gcaacgctgc tataaagacc ccaatcacaa
     3181 gccggaattg atggttgccc tgggtgattt ctggttgtta catggattta agccaacaga
     3241 agaactgaca gatacattat tgaatatagc agaacttagg gaactactgc ccgtttttaa
     3301 taattcaggt tatccaggtt tgtttaagca tgtaatggaa attacccagg aagaggtcaa
     3361 cagaattttg aaacccctga ttgatagtct gaataaaatc tatgaatcag gtgaaatgga
     3421 aaaatcaagt cccgattatt gggctgtcaa agcacacaaa acgttttcat cagacggaaa
     3481 tattgacagg ggtattttct ctatttattt cttcaacctg atccacttga aaaaagggga
     3541 gggaattttc caggccgcag gcgtaccaca tgcttacctg gaggggcaga atgttgaaat
     3601 tatggccaat tctgataatg tccttcgggg cggattgacc acaaagcaaa ttaatgtgaa
     3661 ggaattgatg aaacatgtga aatgtgaggc tacctacccc caaatcctgt cgccggaagc
     3721 agataatgaa ggtgaaagaa agtatgaaac cccggtggat gattttcggt taagtgtttt
     3781 tgagctggag gctggcgata ttgtttcttt ttatccgcag gaggtagaaa cagtattatt
     3841 gacggaagga atcacagaga ttgacaatga gaatgacact atacgattgc aggcaggaag
     3901 tcctgccgct gttgtttttc cgggagataa agtgtacctg gccgccgctg caaaatcctt
     3961 tgttttcagg gcttcgggta gaatccacaa tcgttgataa atagctcact gaattaagcc
     4021 atttctgcta tttttgttgc tcatttaact accagttatg aagtttaacg gaaggatgct
     4081 tgtgtttact atagcactga ttgctctcgc tacagcctgt aaatttttct ttggtcctga
     4141 tcttgactgg tcaggttttt cacctgttat tgccatagcg ctgtttgccg gctttattat
     4201 aaagcataag gacatgtcgt tcataatgcc tttgctggcc ctttttataa gcgatgctat
     4261 cattcatttg ctttataaga ccggaaattt tgagtatgcc ggtttttatg gaggccagtg
     4321 gaaaaactat ttgttgattc tttctgctac gctgcttggc tggattttca aaggtaacag
     4381 ctacatcggt ctggttgcag gtggtattat tgcccctacc gtttattttc tgctttcgaa
     4441 ttttatggtt tggcaggcta caacagaagc ggtatatgca aaaagtttta atggactaat
     4501 gacatgttat gaggcaggtc tgcccttcta tagaaattct ttggcagcca tgctggtttt
     4561 tcttccattg attcttttgt cctataacta tgttacaggt aaaaaggctg tgttgactac
     4621 tgcataatct aacagcaaaa tattttcagc ccatcagtgt ttaactggtg ggttttcttt
     4681 tgagcatatt caggcaaaat tatcaggatt tattctcgta gccatcatct gtcaagagag
     4741 tatgatgatg tccgtcagct gcttcggtag ccagttcatc gcatcggttg ttgtaaggat
     4801 tgtctgcatg gccttttacc catttaaaac gaatatgatg tttttgagat agctcataga
     4861 aatccttcca cagatcagga ttttttttgc ctcctttaaa attggttttt atccaatttt
     4921 tcaaccagcc ttcttcccat gcttttacta catactggct atccgagtag atggtgacat
     4981 gcatgtcttc tttttttaat gctctgaggc ccgcaataac agccatcaac tccatgcggt
     5041 tgttagtagt cagtttataa ccttgcgaca attctttttt atgatttccg ctcataagga
     5101 taacaccata accgccgggt cccgggttgc ctcttgatga tccgtccgta tatattgtca
     5161 cgttatggct cataattaaa cctggaagac gcctgttctg agttcattga tctccttatt
     5221 atgattagcc gcattaatac cttcgctgat cattttcctt gtctcagtcg ggtcaattat
     5281 agcatccacc cataacctgg ccgcagcata ataaggcgaa gtttgtttgt cgtaacgctg
     5341 cttgatttta tcaaacattt tcttttcctc ttcttcagag agcttttcac cttttgcttt
     5401 ctttgaagct acttcgattt gcaacagggt tttagctgct gaatcaccgc tcatgactgc
     5461 aattttagcc gtaggccagg cataaataaa gcgggggtcg taggctttgc cgcacatggc
     5521 gtagttgccg gcaccatagc tattacctgt tacgatggtg atttttggta caacggagtt
     5581 ggctacagca tttaccatct tggctccatc tttaatgatg ccggcatgtt cactgcggct
     5641 gcctaccata aagccggtta catcctgcag aaataccaat ggtattttct tttggttgca
     5701 attaagaata aaacgggcag ctttgtctgc actgtcatta tagatcaccc cgcccatctg
     5761 catttcgcct tttctgcttt ttacaatttt cctctgattg gctacaacac caacagccca
     5821 tccatctatt ctgccgtaac cgcaaacaat agttttacca taatccgcct tgaactgctc
     5881 aaagtttgaa tcatccacaa ttcgttcgat gatatccagt acatcgtatg gtttaccatc
     5941 actgggaaaa attccatata actcttccgg gtttttttta ggcagttttg gagtaatgcg
     6001 atcaaagccc gccttttctt tttcaccaag catggacatc attcttttga tatggtccat
     6061 gcattcctgc tcggttttga atttataatc tgcgatgccg ctgatctcag tgtgtgttgt
     6121 cgcaccacct aacgtttcat tgtcgatgtc ttcgccaata gcagctttta ccaggtaagg
     6181 gccggccagg taaatagatc cattcccttc caccatcagt gtttcatcgc tcattatggg
     6241 caaataggct ccaccggcta cacaaggtcc catcaccgcc gcaatctgag tgatacccat
     6301 accgctcatc cgtgcattgt tgcgaaagat gcggccnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnntac cggtaattgg aaaccaggct cctgctttaa
     6481 ccgtttgatc attggctaca atgacacatt gacgcccact gacataaccg actcccgcaa
     6541 cggtgccacc ggcggggcat ccaccttgtt cttcgtacat ttcataccct gcaaaggcgc
     6601 cgatttcaat aaaaggttta tcgggatcaa gtaagtaatc tatccgttct ctaggggtaa
     6661 gcttattttt ctctctttgc ttttcaatag attttttccc gcctccttcg tagatattct
     6721 ccagtttacg gcgcatatca ctgaggcttt gcttcagttg gtcttcgttt cggttaaatt
     6781 caaggttcat acaatcatcg ttaggcagca aagataaggt tgctggtata agtccttaaa
     6841 tgatgtggaa ctcaggtaat tttatttagg gcaagatgct attttttgct taattacagt
     6901 gattaaagca ctgtccttat tttcttctgc taaaagaaat gctttttcga aatatctttt
     6961 tgcttcaact ccttcattaa gatttaaagc attttcaccg cttttaatcc acatgtatgc
     7021 tgaggaggga aatctctctc tccctgtact gatagctgct tttgctgccc gatattcatg
     7081 tacttccgtt agattatcga aaactgagaa gaacagaaat tcatttgcgg cttcgatatt
     7141 gagttgcgaa agaacagagt cagttataac tggaatattg ttagttggtt gctttaacaa
     7201 ccagtctgca agcctgattc tgtcagcatg aattggcttg ttatattttt tgttgttgag
     7261 gatagcaaat gcccggccag ttagctcgta tgcagcgcca tattggttgc tgcaccacac
     7321 cacaatatcg cctttataat aatcaaaata tacatctatt gtaaatccct tgcctccgcc
     7381 attatgtcca gctatgaatg ttttttcagg tttatctttc aacaccgcca tacccaatct
     7441 cctgtannnn nnnnnnnnnn nnnnnnnnng ctgtcaattg tatgattatt ggtcagttga
     7501 atttccatcc attttcttgg cagtaatttt tcattcaggt atgcttgtgc aaacaaaaac
     7561 aggtcttctg ctgtagagat ccacccacca gctccactgg ctttcccatc accatatttg
     7621 gtaatatcgg ggctaaaaag tttttcagtt atttgataat aaggcctggc tgcatttgat
     7681 ggcaaaacag aatcatttaa tgatcttgtt tgctgcatac ctgcaacata gaaaacattc
     7741 tttttgatgg attcttcaaa agacatatta tctgtattgg ttatgatttc ttccaataag
     7801 acatatccgg aattgctata ataactgcgt tcgcccggag tagtgaagat tacaggggct
     7861 tcctctataa atttttttat atcattccgg gtataaatct ttccaaattc atatgccggg
     7921 tgttcccatt ggctgctcag ccccgaagta tgagtcagga ggtgctggat agtcattttg
     7981 tctgcattgg gaattttcca atcaggtaaa agtttggcaa cgctgctgct taaggatagc
     8041 ttacctgctt taattagttg aaggattcgt attgcagtga acattttacc aacagactca
     8101 gttttgaaag ctgtattcag ttgcatgggt gcccgggttt ccctgttgga aaatcctaat
     8161 gtagcggaat aagttatctt atccttattt ccaattagta atacaccgtt ccagtttagc
     8221 tgttggtact gttttatcag ggaatcaaac ctgctttgtt ggagctgggc actgcagcaa
     8281 acagtgaaaa aaaatgtcag aacaacaatg cacagatatg ccggattaaa ctgctggatg
     8341 tttttttgat tgaaaaaatt gtttgcttcc attttcttca tctcattttc cctttactat
     8401 taagtatttt caaatatcaa taagataggg gtgtaagggt atcacataaa gatgtgaata
     8461 ttgtagcagg ccgtttttca ataatggtaa ttaaattatt tttagggaca aaagctgatc
     8521 aatatgacac ctgccgcccg ttctgtctgc tatttcggat attatctata tgtagtaggc
     8581 ctgtcactca tcacagttcc caatttattt ctgcgtacca tgcaattgcc ggaaaccaat
     8641 gaggtatgga tacgaatcgt tggggtgctt gtattctgtc ttggttttta ttatcacagg
     8701 agcagtgtat ataataatgt gcctctttta aaattgacca taccggcgag gatttttgct
     8761 gcgctatgtt ttttttcatt tgtactgtta aacctggcac cacctatgtt ggctgtgttc
     8821 ggtgctgttg atttgatggc tgcagtttgg acatttattg cttttagaaa ttaattttca
     8881 ataaatcatt aagggaaaaa atttgaaaac gggattgaga aaaggctatt gaaactggaa
     8941 aagaaggtaa gcaggcgcag aaaagtgaat attgtgttgc tcggcggaat actgcttgcc
     9001 acgttgtatg gttttaaaag gaccaggcgc tggaagattt aaaagttaga agtatcgcta
     9061 tttcgaacga caaggggaac aattatacat ttatctccgg aagccagaaa tacggttgct
     9121 ggatgaacat ctgtgacaag tatggaagaa atacggtatt gctgccacag aagaaacagg
     9181 gaggaggata tattaggttt aaaaaataca tcaagccggg gagtagtttc aaagggtact
     9241 gcagaagatg gtgacgggta tagtacaact ggttattttc ccaggtaatt tctgaattat
     9301 tttaatcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa gcagaaggta
     9421 aaatcccgga ctatcaagtc atctgatttt gaaagtattt gtgaattgca actgacctgt
     9481 ttcccgggta tgaaaccatg gagcaggcaa cagtttgata gtattctttc caattttcct
     9541 cagggacagt tttgtgtgga atatgatggc aagctagtag cttcaagctg tagtcttatc
     9601 atcaacagat cacaataccc ggatacggca agttggaatg aactaaccaa tacaggttat
     9661 atcactaatc atgatccgga aggtgataca ttatatggca tggaaataat ggttcacccg
     9721 gaataccgtc tgatgaaact ggcccgacgc ctttacgagg caaggaaaaa tttggttaga
     9781 gaactcaatc tgaaaactat tctcatcggg ggacggatac ccggctacca tttgcatagc
     9841 ggcagtatgg atgtagagga gtatgtacag catgtaattg ataaaaagat ctatgatgcg
     9901 gttctgacgg cacagctttc taatggcttt gtattgaaaa gattattacc gggttattta
     9961 cccaatgata aagaatcctg tggttatggt acttttctcg aatggagcaa cctgatgtat
    10021 acagatataa aaaaaccagt gaaaccggat ccttatgtgc gaatatgctg tatccaatac
    10081 cagctcagaa tgattaatag ctttgaagag tttgcagaaa gctgcgaata ttttgtggat
    10141 gtagcttcag attatcgctc agattttgtt gtatttccgg aaatgctgac tatgcagttg
    10201 cttacattcc ttccgcaaaa acctcctgct gaggctgtac gtcagctgga ccagtttact
    10261 gaacagtaca ttgaaatgtt ttcaaaattg gccatccgtt ataatgtaaa catcattggt
    10321 ggttcacatt ttattattga gaatggggac ctttataatg tgtcctatct tttcaaaaga
    10381 gatggaacat gggcaaggca gtttaagata catatcacgc cacatgaaaa gaaatggtgg
    10441 ggagtaaagc ccggtgatga aatgaaagtg ttcaatactg actgtggcaa aatagctatt
    10501 gcaatttgtt acgatatcga attccctgaa atagtaagag tagcagcaag taaaggggcc
    10561 ggaattattt ttgttccttt taatacagat gtaaggcgtt cctatttaag ggtaaggtat
    10621 tgtgcacagg caagagcaat agaaaaccag gtttatgttg tgattactgg ggcggttggc
    10681 aacctgccca gggttgagaa cctggatatt cattatgccc agtcagcaat attgacacca
    10741 agtgatatcg aattccaccg ggagggatta gctgcagaag caccggctaa tgttgaaaca
    10801 gccattgtac aggaactcga tattaatcta ttgaaacgca acagggaatt tggtagtgta
    10861 cagacatggg ccgaccgaag atccgatctc taccatgttt ctttcaagga aggagataag
    10921 cagctccgaa tataattttc tgaccgtaaa tttatagcac aactaagcca atgacaggaa
    10981 tacacatttt gattgcagaa gatgaaaaaa agattgcaga tgcattatgc cagggccttg
    11041 aagaaaatgg ctttacggca gcggtggctt atgacggggc aatcgcacta aaaatgttcc
    11101 gcaatattcc attccagtta gtgatcgcag atattaatct tcccggactt aatggttatg
    11161 aactatgcca cgccataagg gcagccaatg cctctgtacc tgttgttatg ctcacggcta
    11221 tgagtgccct tgaggacaag attgaagggt ttgatgcggg agcagatgat tatattgtta
    11281 agccctttga ttttcgggaa ctgctggtgc gcataagggc actgctgaaa cggacacata
    11341 caacagttgc aacaggaaca ctattacagg ctgatgattt ggtaatgaat ctcgacagta
    11401 agcaggtcac aagaaaaggt cagccaatac aattgacagc aaaagagttt caactgcttg
    11461 aatatttttt aagaaataag aaccgggtag tttcccgggc ggatattgct ttgcaggtat
    11521 gggatattga ttttgatacc cgtaccaatg tgattgatgt atatgtcaac tacttgcgaa
    11581 agaaaattga caagggattt gatcagccgc ttatatatac acaggtaggg atgggttatg
    11641 tattaaaaga caagagctga tgaagcttgt atataaaatc aacctgttat ttgctgcaat
    11701 agtctctgct attgtattag ttattgcttt tttgatttat accatcagcc gtcaaaccct
    11761 ggagaatgat taccgccagc gacttaaaac aagggctgcc cgtacggcgc aactttatac
    11821 ttttttcaga tcagatacca cgaacctgct caaatcgcta gacgccaatg caccgcctgc
    11881 cttgtttaat aaaaatatca acatatatga tgataacagg aacatacttt atgagtacca
    11941 cgacgaaggc acacaaaggc ttagccctga ttctaaaaga tttgctgaag ctgctgaaag
    12001 tggtgaaatt ttttattcag tgaatggaaa agatgtatgc ctgtattatc acaaagatcc
    12061 cgttatgaac ctgactgtgc tggtagctgc agataatctt gcaggtaagg attatatcag
    12121 gaacttaaag aatatttttc tgctattttt ccctgcagca gtcattattg cattggctgc
    12181 aggttatctg ttctcaagga gtataataag accgataaag gaaaccatac gggatgtgaa
    12241 acttatcact tctcaaaacc tctcacatcg tcttttcgaa ggaaacaaaa aggatgaatt
    12301 cagccaattg aatgctactt tcaatgatct gctgaaccga ctcgaagaat catttgctat
    12361 tcaacgcagg tttatatcca acgcttcaca tgaattatcg acaccactca cctcggtttc
    12421 cagccagatt gaggtatcgc tgttacaaga tcgttctccg gaagaatacc ggcatgttct
    12481 gcgatccgta ctggacgatg tgagggagtt gcaccaactg acccacaacc tccttgaaat
    12541 tgctaaggcg ggtacagatg gcggcataac actggataaa gtacgaattg atgaagtgct
    12601 tataaaagcc cattcagatt tgctgcgaca gaataagggt tatagagctg agctgttttt
    12661 tgatgaattg ccggaagatg agaatgaatg tcttgttttt ggaaactatc atttattata
    12721 tagtgccttt aaaaacctgc tggagaatgg ctgcaagtac tcgccggatg catccgttaa
    12781 agtgtatttg tcctttcgaa cacaagaagt tatgataacc tttacgaata acagcgatat
    12841 gctgactaac gaagagatag gtaaactatt tgaaccattt tacaggggta gcaatgnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atctgttagc tccacggcag aacaaggaac
    13021 cattctcgtt atactactgc ctgctctccg gaaataaaat tttaattcag ttctaatctt
    13081 agcctaattc caggtttaga cactgcaggt attttgtgat tcaaatacac aggtagtttg
    13141 aaaaaactgt ttaccaagat actggtaccg gtaaatttta accggaatac ggcaatggcc
    13201 atgcagaaag ctgtgcaggt ggccaacgaa tttcattgtg atgttcatgt actgcatgta
    13261 caaacacccc ttactatctt gccattttta tatgaaggtt tttttgccac aaatgtttat
    13321 gagtcacatg ccaggacagc agcaggaaaa cttcagcaat taagagaaat ttatggtaag
    13381 aagctaaatg acggcttgct gatgacaatt tcgcaggaac tcggtgactg gcaaacgatg
    13441 ttgaagaaga caatcatttc ggagcatacg gacctggttt taatacccaa gcatcccaaa
    13501 cgtttctttg gtgcattgat tcaaaaaatt aatatcaata aacttataca gcaaacaaaa
    13561 tgtcccgttc ttactgttac tcgacgattt aatattcatc acatgcaaaa tattgtagtg
    13621 ccggtgggtg atactttacc gatacggaaa ttaacgatgg caacttattt agcccggcag
    13681 agcaatggtt atgtacacct gatgggcagt tcaggaaaaa gcaagagaga agagaaacag
    13741 agtagctggt gtgtgacgag ggcgtatcag ttattaaggg attatacaca agtgaatatt
    13801 cattgtagta ttggtgccga caataaccgc atggatggta tgctggccgt tgcaaggaat
    13861 gttaaggctg atctcatcgt tgtaaatagc ggcaaagaga cgcagccggc cggttggctt
    13921 aaccgtctat tagggaaata tgtgtacaaa gaatccaata ttccggtact gactgtagcc
    13981 ggaacaaata attaaattat taaaataata taatgatgaa aaaaattacc ggaaccctta
    14041 ttatcacttt tatgttttcc actgtattag tggcacagga tgctgcggag gtagaacaat
    14101 tgctggacaa tgaacgatat gaaagtgctg aagccatttt ggaaaaaaag ctcaacacag
    14161 acggtgtaat gccggatatc agttacctgt tggttaaaac ctatcttgaa caggacaagg
    14221 tagaggaggt aaaggatttt atttcaaaat acaagttgag tgatagcccg gaatctgatc
    14281 cgcttgacag gattacttat gcaaggtatt tattgcaaaa aggggacaag gccggggcag
    14341 atgcaatttt taattctatt ctggaaaata aaaagaaccg aaaaaatcat tgctcatggc
    14401 tatggccgaa gtgaatattt cagagccaaa gggtgatgca tcacttgcat tggaatggtt
    14461 aaaaatggca gctgataaag atgacgacaa tccatccatt ttcatattag aagggcttgc
    14521 atatcgtaaa ctttctgatg ccagtaaagc ctacttggcg taccagcagg cgttgaagaa
    14581 ggataagaat aatgtaaagg cacattacct gcttggcaaa atatttacag ctcaaaaaaa
    14641 ctccgaagta taccttgaac acttcatgaa agcatatgaa gttgattcaa cttatgcccc
    14701 ggtcctggaa gcactatatg atcactatta caatgtcgat gtaaggattg cccgtaaatt
    14761 ccttgaaa
//
LOCUS       DAORLS010000037        14748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2188_length_14748_cov_2.304158, whole genome shotgun sequence.
ACCESSION   DAORLS010000037 DAORLS010000000
VERSION     DAORLS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14748
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2188_length_14748_cov_2.304158"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PK977_04645"
     CDS             <1..1005
                     /locus_tag="PK977_04645"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRF17429.1"
                     /translation="EPVHHFSMNGLRLLCSLGFKKILAKMFRPKEDHNYTAFGISFPN
                     KVGLAAGFDKNAKYLRELECLGFGCVEIGTVTPRPQAGNDKPRVFRLPKDKALINRMG
                     FNNEGVKVVAERLSNWRNSQLPAHDARLIVGGNIGKNKVTPNEDAWKDYEICFKELYP
                     YVDYFVVNVSSPNTPGLRELQEKESLRKILTHLQQLNVTLSLSKGAKPLLLKIAPDLT
                     KEQIDDVIDLAMEIKLDGLVATNTTISREGLTTSGSRIKTIGAGGLSGLPVKQRSTEV
                     VRYIHQKTNGQIPIIASGGVFTGTDAKEKLEAGASLVQVWTGFIYEGPGIVKKICRYL
                     "
     assembly_gap    1148..1157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1158..>1446
                     /locus_tag="PK977_04650"
     CDS             <1158..>1446
                     /locus_tag="PK977_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRF17430.1"
                     /translation="RLKDPKEQRRARRVWMITGIISRSLLLMALSWLLSQKGKSVFTL
                     WGKGFDLASIIMLAGGLFLIYKSVIEIHHKLEGEDPNVALKKKNGISLSQAI"
     assembly_gap    1447..1528
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1529..1861
                     /locus_tag="PK977_04655"
     CDS             <1529..1861
                     /locus_tag="PK977_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRF17431.1"
                     /translation="IMIAAVVIAMIVMFLFSPAISGFIHKHPTLKMLALSFLVMIGLG
                     LVIEGWDSEAAHQLHLKNYIYFGMAFSFTVEMLNMTMLSRAAKKKAHLVELNEPVLEE
                     EKKQSEVH"
     gene            complement(1847..2548)
                     /locus_tag="PK977_04660"
     CDS             complement(1847..2548)
                     /locus_tag="PK977_04660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRF17432.1"
                     /translation="MILPFFYINNYSGQKQLPLDEETSRHVVQVLRMKEGEQLNLTDG
                     KGKLLTCEIKEAGKKSCYVEVKATSNRLSNARNISIAISLLKNTNRFEWFLEKATEIG
                     VSEIIPLVCHRTEKEKFRHDRMNAICISAMLQSQQCWLPVLHAPVEFEKVFSKISSEQ
                     KFIAHCDESSTKNNLSTFQPFNSSTILIGPEGDFTPKEIEIALQSGFQPVSLGETRLR
                     TETAGVVAAAVLMNL"
     gene            complement(2623..4197)
                     /gene="dnaB"
                     /locus_tag="PK977_04665"
     CDS             complement(2623..4197)
                     /gene="dnaB"
                     /locus_tag="PK977_04665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRF17433.1"
                     /translation="MDLTNLNKDRTGKQRRKSTLDLGTMVYGKVPPQAKDLEEAVLGA
                     IMLEKSAFDTVVEILKPECFYVEAHQRIFKAMQSLSNKSQPIDILTVAEELRFKEELE
                     MIGGAYYVTKLTNAVVSSANIEAHSRIILQKFIQRELIRISGEIIGDAYEDSTDVFDL
                     LDDAESKLFEITNNHLRKNFDTIDSVLVKTIQRIEDLRHKNEDVTGVPSGFPSLDRVT
                     YGWQNTDLIILAARPAVGKTAFALNLARNAVMNPAKQTPVALFSLEMSAGQLVQRILS
                     AESEIWLEKIARGKLEEHEMKQLYARGIQKLAQAPLFIDDTPALNIFELRAKCRRLKN
                     KHNIGLIIIDYLQLMSGTGENRNSNREQEISNISRNLKALAKELNVPIIALSQLSRAV
                     ETRSAGKDGNKMPQLSDLRESGAIEQDADMVMFLYRPEYYDITQNEMGESNRGETHVR
                     IAKHRNGSLETIKLKALLHIQKFTEDDGSGGFGATGLPGNWKPVSDVDGEGGARVFIQ
                     TGSKMNDMGDDEDTPF"
     gene            4614..5114
                     /locus_tag="PK977_04670"
     CDS             4614..5114
                     /locus_tag="PK977_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17434.1"
                     /translation="METTKRPRPVLTQTQNNTTLWIGHMQTDPNDHLAGQTFLCPAGG
                     VLNNIQVFAEAVQNPGEVELTLHEFDPATKTWGPAISQSNLLLHKGDDSRWIRFETPA
                     VELEQGATYGFRLHTPDALVALGEAVSHAHKPFTFGQAWNGHAPSAKGHYSTYFSLAF
                     KVELCA"
     gene            5360..>6668
                     /locus_tag="PK977_04675"
     CDS             5360..>6668
                     /locus_tag="PK977_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17435.1"
                     /translation="MRNSLFLSLAILLFLSANSQKPKILPVKPYKPSVILYEHANYTG
                     AFKTLGPGQYVLSDFNDVASSIRVPAGMVAYIYEHAAATQGYGISVDFLEDVSDLSVY
                     GFNDKASYISVFYATKDNKYEWKRNANVNGQFVPGHWERKSARPGPANTIAVVSPHVP
                     GPVAATVLAVNGANTVISTLGNQSAEGKTLWETAKLQMGIIGNDYRGREEIGTAAFER
                     ASNNTFIPDNFNFWYPQRPPNDHRNPAKRTLSGQVIKAGQVNIQGTFADFDVNIDIVP
                     HPKYEYLVTTAHKPEFTTIMKLQDVAYDIKDCPPSFHAVEAEIAEDYWPKGNHTFGRA
                     RLTDMVLSRVSRGICVYGTWIYDMGHCCHPEIHPAEQLWWSDSSENRIKSNLNVVCDA
                     SRRFWWRSQMDDGTKLKPWAEPPIKGLFAIAFEYALPNAAATAS"
     assembly_gap    6669..6685
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6686..>6931
                     /locus_tag="PK977_04680"
     CDS             <6686..>6931
                     /locus_tag="PK977_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17436.1"
                     /translation="KFEADYIQHYNLAEYPNANQTYNLVYNGKNIVSFVPNNNAFKVS
                     FEHVGISPEDNNKIRGFLVLETSVGKTTQIATQDYYPG"
     assembly_gap    6932..7009
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7206..9383)
                     /locus_tag="PK977_04685"
     CDS             complement(7206..9383)
                     /locus_tag="PK977_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRF17437.1"
                     /translation="MLVSTELIKQSVERFKAGDRIKPGEKKGKFIEDELEEYIDKNVP
                     EAVKKGNITGDNDNLANRLQLIEDRAPEPASFAFERAIGKNDSVYSNFVELIASTKQK
                     VARVVIKEGPRTIGFATGFMVSSRLLLTNWHVFRKKEYVMQSEAQFNYEYDIYGRPKQ
                     PVSFRLRPDDFFYSLKALDYCLVAVEPVDVSGNAQLSAISYIFLDPTPGKLGDEDVEL
                     LNIIHHPGGDYKQLSIRENQFKKIMPTTLWYKSDTAQGSSGSPVFNDQWQVVALHHMG
                     VPSMSADGKNFLDIDGNIIPVVNGEVDETKIHWIANEGIRISVILKDIFSRFPHSDYV
                     NELKRRPFSPLELPSGVVNWDKKNQPDPIGGWAGNNDVSIVFPASLLEASGQVTVSIR
                     NKGDDVAARPDKILSDIDTTIAEAKKADKEKDVVFSGCKGYDPGFMGVNIPLPKPQKS
                     LMKNIAKMSNGKSELKYFKYSVLFNETMKMPALSAINVEGNPALRLDNSKRTDDWLRD
                     KRIDISKQLTDKFYAGSGFDKGHMSRFEDANWDKTEEAALRNGIYTCFYTNACPQVPG
                     LNRGGGIWGKLEKAILEKGVKKEKGSEGRITVFNGPIFSSNKKKFRGEIVPMEFFKII
                     VWRNDDNQLKATAFKLSQEELITGIKFTEVAGMQYEAIDIDTNAHFKEYQCSIRSLTN
                     ATGINFKPLEKYDTFNKTGTNEDVLISSEKDLERMIVMNNKKR"
     assembly_gap    9652..9670
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9671..>10026
                     /locus_tag="PK977_04690"
     CDS             <9671..>10026
                     /locus_tag="PK977_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF17438.1"
                     /translation="VKDTQGNQYVDLVQEGGGVLGIALVGYTYVLEKMGIRFFSMAGT
                     SAGAINTMLLACAGNKEEEKSSKIVEHLVKLEMFSFVDGKSSNWKFTKWIKRVIQKML
                     LGSNMFKKISRIATVI"
     assembly_gap    10027..10038
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10039..11080
                     /locus_tag="PK977_04695"
     CDS             <10039..11080
                     /locus_tag="PK977_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF17439.1"
                     /translation="LLTVGCFVINFIWPGVAKWIGLGAGLLLISVIVFFFIAAAKFKE
                     FAREGYGLNEGKAFHNWIKGVISSFHVRNDVAMQKINNLADFSYHFMRVPKLEVIKDD
                     RRENNDPPHMPMLTLITCDIIANRKIEFTQMWDLYWNNQEEVHPGDFVRASMSIPVFF
                     ETYTLSDIRKKSSFDIWDKHLNWQNQQKRIPDKVQFIDGGTLSNFPINVFYNPRYPVP
                     RMPTFGIRLQDGVMDAASRSNNSLFKYIFSLFSTIRFNFDRDFITKNRAFNYGVKNID
                     VQEHSWLNFFMKDEEKIILFRKGAQAAAEFLKTFDWAQYKDERLKNFTMHQDRFDNPN
                     NLEVFPFSDRNS"
     gene            complement(11147..11512)
                     /locus_tag="PK977_04700"
     CDS             complement(11147..11512)
                     /locus_tag="PK977_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF17440.1"
                     /translation="MKKQLLIMLLCLFLVQAGVEAQLRKQARHQQEPLTTELQANFSE
                     TGRYVFVQNQLLLTNLKEEEFDRIAIINMQGSVLQRQNVSGPAVRLNLSELEEGVHLL
                     LLHSSSRMKEKTIKMVVKR"
     gene            complement(11642..13297)
                     /gene="recN"
                     /locus_tag="PK977_04705"
     CDS             complement(11642..13297)
                     /gene="recN"
                     /locus_tag="PK977_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRF17441.1"
                     /translation="MLTRLSINNYAIIDELEIDFSGKLSIITGETGAGKSIIIGALGL
                     ILGERADSSSLVNKEKKCIVEGTFSGNSKKEVTDFLKANELDAEEEVVLRREIGTNGK
                     SRAFINDTPVNLVQLQQLSSLLIDLHQQFDTLELGETDFQRQVLDALAGQLILIEEYQ
                     VQYRQWLQLRKETEELKVQQQQFEKEADYNRFQFAELEEAAFKENELEETEAELKLLN
                     NSEGIKAALAKTYFDLRESEMPLVQQLKILLNQLNVYSSYHPDLPALLQRLQSAQVEL
                     QDIADDVDRISNHINYDPDKIEQLNERLSLGYKLQKKHGVSSTAELLAIQKELEEKLQ
                     AVLNIEETIQQKEKETNRLRAEAKKTAEKITAGRNKQVKPLEDKVNKLLAQVGMPNAK
                     LKVLVQPADLSSSGADAIEFLFDANRSGQFQPVRKVASGGELSRLMLCIKSLVAQSID
                     LPTLIFDEIDTGISGEAARQVGIIMKELAAKRQVICITHQPQIAGKADAHFFVYKEIV
                     RDKVKTNIRQLTTEERITAIAKMLSGEKPTAAAMENAREMVMN"
     gene            complement(13437..14192)
                     /locus_tag="PK977_04710"
     CDS             complement(13437..14192)
                     /locus_tag="PK977_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRF17442.1"
                     /translation="MAHVKEFSVEEKLTSLVTLQKIESKLDELHILKGELPMEVADLE
                     DEITGLHARQTRIEEEINGVTEFIEQRKNAIKESEALIKKYEKQSANVKNNREFEAIN
                     KEIEMQQLEIKLAEKHIKDATEEIAEKAVLLEKAKKNISVKEGVLSTKKGELEKIIVA
                     NEKEEKHFNKLAVEAREHVDSRLLASYEKIRKSYRNGLAVVPVERDACGGCFNAIPPQ
                     KQSEIRQHKKVMICENCGRILVDEELHNNVEVK"
     gene            complement(14215..>14748)
                     /locus_tag="PK977_04715"
     CDS             complement(14215..>14748)
                     /locus_tag="PK977_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRF17443.1"
                     /translation="RGKRHKEQEVKIEVIFPAWLESRLVSVMKAAHTYEEVAYDIFPL
                     SNKYQGFGSGVIGELPASLDEKKFLARIKRVFGVPVVRHTAYLNKAVKKVAICGGAGS
                     FLISKALAVGVDAYITADIKYHEFFDANGRLLIADVGHYESEQFTINLLQEFLEQKFP
                     TFAVLKTGVRTNPVHYF"
BASE COUNT         4002 a         3204 c         2876 g         4448 t
ORIGIN      
        1 gaaccggtgc atcatttttc catgaacgga ctcaggttgc tttgcagcct cggtttcaaa
       61 aaaatactgg caaaaatgtt caggccaaag gaggatcata attataccgc tttcggtatt
      121 tcatttccta acaaagtggg acttgctgcc ggttttgata aaaatgccaa atacctgagg
      181 gaactcgaat gcctgggatt tggctgtgtg gagatcggaa ctgtaacgcc acgaccacaa
      241 gcaggtaacg acaaaccaag ggtattccgc ttgccaaaag acaaagccct gatcaaccgg
      301 atgggattta ataatgaagg cgtaaaagta gtggcagaaa ggttaagtaa ctggcgaaac
      361 tcccaacttc cggctcatga cgcacgactc attgtcggtg gaaatattgg aaagaacaaa
      421 gtcactccga atgaagatgc atggaaggat tatgaaattt gttttaaaga gttgtatcct
      481 tatgtagact attttgttgt gaacgtaagt tcgcctaata caccgggttt aagagaatta
      541 caggagaaag aatcactccg aaaaattctg acacatctgc aacagctcaa tgtcaccctg
      601 agcttgtcga agggtgcgaa accacttttg cttaagattg ccccggatct cacaaaggaa
      661 caaattgatg atgtgattga cctggcgatg gaaataaaat tagacggact agttgctacc
      721 aacaccacta tcagccgtga agggttgacg acatccggct caagaataaa aactatcggt
      781 gcagggggct taagtggttt gccggtgaaa caaaggagta ctgaagtagt acgttatata
      841 catcaaaaaa caaacgggca aataccgatt attgcgtcag gaggagtttt tacaggtact
      901 gatgccaaag aaaaattaga agcaggagct tctcttgtgc aagtatggac cggctttatt
      961 tacgaaggac ctggtattgt aaaaaagatt tgccgctatt tataaaccaa taactaaatc
     1021 ttaactccac aatcttaaat aatcaatgga acacctattt acagccgata gtataatcag
     1081 ttttgttatt ctcgtcattc tggaagtggt gctgggaatc gataatgtaa tattcgtttc
     1141 tctttctnnn nnnnnnnagg ttaaaggatc caaaagagca acgccgtgcc cgccgtgtat
     1201 ggatgataac cggtattatt tcccgcagtt tattgctgat ggcattgagc tggttattat
     1261 cacaaaaagg gaagtcagtt tttacactat ggggcaaagg ctttgacctg gccagtatta
     1321 tcatgcttgc cggcggctta ttcctgatct acaagtcagt aattgagata catcacaaac
     1381 tggaagggga agatcccaat gtggcactaa agaagaaaaa tggtatttca ttatcgcaag
     1441 ccattannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat catgattgcg gccgtagtaa ttgccatgat
     1561 cgtaatgttc ttattcagcc cggccatatc aggtttcatt cacaagcatc ctactttaaa
     1621 aatgctggcc ctttccttcc tggtaatgat aggacttgga ttagtgattg aaggatggga
     1681 cagtgaagcg gctcaccagt tgcacctgaa aaactatatt tattttggta tggcgttctc
     1741 attcacagta gagatgctga atatgaccat gctcagccgg gcggcaaaga aaaaagcaca
     1801 tttagtagaa ttgaatgagc cggtgctgga ggaagaaaag aaacaatcag aggttcatta
     1861 atacagcagc cgccaccact cccgccgttt cagtccgtaa tcttgtttcg cccaggctta
     1921 ccggctgaaa gccgctttgc aatgctattt caatttcttt gggggtaaaa tctccttcag
     1981 gaccaatcaa gatagttgaa gagttgaaag gttgaaaagt tgacaggttg tttttggttg
     2041 aactttcatc acaatgagct ataaactttt gttcggagga tatttttgaa aaaacttttt
     2101 caaattcaac aggcgcatgt aaaacaggca accagcattg ctggctttgc aacatggcgc
     2161 tgatgcagat agcgttcatc ctgtcgtggc ggaatttttc tttctctgtt ctgtgacaaa
     2221 ccagcggaat aatttcgctc actcctatct cggtagcttt ttccaaaaac cattcaaagc
     2281 ggtttgtgtt tttgagcaaa gagatggcaa ttgaaatgtt tcttgcattg gatagccgat
     2341 tactggttgc ctttacttct acgtagcatg atttttttcc tgcttcttta atttcacagg
     2401 tcaataactt tcctttacca tcagtaaggt tgagctgctc accttctttc atgcgcaata
     2461 cctgcaccac atgcctgctg gtttcttcat ccaaaggcaa ttgcttttgc ccggagtaat
     2521 tattgatata aaaaaatgga agtatcataa aaaagtcccg cggttgcggg acaaatctaa
     2581 aatcttaaat ctgcaatcta aaatctgcca gaggcagaca ggtcagaatg gcgtatcctc
     2641 atcatcaccc atatcattca tcttgctgcc agtctgtata aatacccttg caccaccttc
     2701 gccatccaca tcgcttaccg gcttccagtt gcccggtaaa cctgttgcgc caaagccgcc
     2761 acttccatca tcttctgtga atttttgtat atgcagcaat gctttcagct tgatcgtttc
     2821 caacgaacca ttacggtgct tggctatgcg gacatgggtt tcgccccggt tgctctctcc
     2881 catttcattt tgcgtgatgt cataatattc cgggcggtaa aggaacataa ccatatctgc
     2941 atcctgttcg atagctcccg attcacgaag atcactcagc tgcggcattt tgtttccgtc
     3001 tttacccgca cttctcgttt ccacagcacg gcttaactgt gaaagtgcaa tgatcggtac
     3061 attcagctct tttgccagag ctttcaggtt tctcgatata ttactgatct cctgttccct
     3121 attgctgtta cggttttctc cggtaccact catcagctgc aaatagtcaa tgatgatcag
     3181 gccaatattg tgcttgtttt tcaaccggcg gcacttggca cggagttcaa agatgttcag
     3241 tgcaggtgta tcatcaatga ataacggagc ctgtgccagt ttttgtatac ctcttgcata
     3301 caactgtttc atctcatgct cttccagctt accccttgca attttttcaa gccatatttc
     3361 gctttcagca gataagatac gctgtaccag ctgcccggca ctcatttcaa gggagaataa
     3421 agccaccggt gtttgcttgg ccgggttcat tactgcattg cgtgccaggt taagtgcaaa
     3481 agcagtttta ccaacagcag gtcttgcggc aagaataatg aggtccgtat tctgccatcc
     3541 ataggtaaca cggtcaagcg atggaaagcc gcttggcaca cccgtcacat cttcattttt
     3601 atgccgaagg tcttcgatac gctggatggt tttcaccaat acagagtcga tcgtatcaaa
     3661 attcttacga aggtggttgt tggttatttc aaacaatttg ctttctgcat catctaagag
     3721 atcaaataca tcagtagagt cttcataagc atcaccgatt atctccccgc tgatgcggat
     3781 aagttccctt tgaatgaatt tttgtaagat tatcctggag tgagcctcga tattggcaga
     3841 tgaaacaaca gcattggtga gcttagtaac ataataggct ccaccaatca tttccagctc
     3901 ttctttgaaa cggagttctt ccgccaccgt taaaatatca attggctggc ttttattgga
     3961 aaggctttgc atagccttga agatgcgctg atgagcctca acataaaaac actccggttt
     4021 cagtatttct acaacagtat caaatgctga tttttcgagc ataatagcac cgagtacagc
     4081 ttcctccagg tcttttgcct gtggcggtac tttgccatat accatggtgc caaggtcaag
     4141 tgttgatttt cttctttgct tgccagtacg gtctttattc aggtttgtaa gatccatgtt
     4201 cgtctatagc gataaaaggt tattcattga gttttttact ttcaatgacc ttgggcactt
     4261 cttccgtctt gctttaagaa atcgttattt ctatgttagt ggaatgtttc cgtccagtca
     4321 ggaaggcgaa tttaaaggcg gacagacatt caaaattgtt tttgaaaaag tttttctttc
     4381 tgcaccttct ttccacccaa aaaccgggtt tatccacaaa tatttattgc tcaaggtctt
     4441 tgtcaattta cacaatttcc ctagtaattg tcccaaaaga tgctaacaga cgtttgcatc
     4501 ttttacgcaa atgacgagaa tcatataccg ggacacaata tgaccagccc cggcagagtt
     4561 agttttgcag agaaatccgt ttacggcttc tcttaagctt aaaaaatata tctatggaaa
     4621 ctacaaaacg cccccgtcct gttcttactc aaacacagaa caatactact ttatggatcg
     4681 gtcacatgca aacagacccg aatgaccatt tagccggaca aacatttctt tgccctgcag
     4741 gtggtgtgct gaataatatc caggtatttg ctgaagcggt acaaaacccg ggagaagtgg
     4801 agctgaccct tcatgaattt gatccggcca ccaaaacatg gggccctgcc atcagccaat
     4861 ccaatttatt attgcataaa ggagatgatt cccgctggat acgctttgaa acacctgctg
     4921 tggagctgga acagggagcc acttatggtt tccgacttca tacccctgat gcattagtag
     4981 ctttaggcga agctgtaagc catgcacata agccttttac ttttgggcaa gcctggaacg
     5041 gacatgctcc ttccgccaaa ggccattact caacttattt cagcctggcg tttaaggttg
     5101 agctctgtgc ttaataaata accgagacgg caagtgaaat atccagccct gttttcctgg
     5161 taataactac caggatgcag ggtttttatt atcataatga tagaatacaa aacgcagctc
     5221 tggaaaatac tttcattgta aatctaaaaa attgtggcaa ctttatattc ctccgcaccg
     5281 tcccgtctac cctgaaggaa aattccactg cctttttgat aactgaaacc aactgattac
     5341 tcaccttata ccattaaaca tgagaaattc cctatttctt tcacttgcca ttttattatt
     5401 tctttcagcg aattctcaga aaccaaaaat tttaccggta aagccataca aaccctccgt
     5461 gattttgtat gaacatgcta attataccgg tgcttttaaa acactgggac cgggacaata
     5521 tgttttaagt gactttaatg atgtggcctc ctccatcagg gttccagcag gaatggtagc
     5581 ctatatttac gaacacgcag cagccacaca gggctatgga atttctgtag attttcttga
     5641 agacgtttct gatctgtctg tttatggctt taacgataag gcttcttata taagcgtctt
     5701 ttatgcaaca aaggacaaca agtatgaatg gaaaagaaat gccaatgtaa atggccagtt
     5761 tgtgcccggc cactgggaac gtaagagtgc aaggcccggt cctgctaata ctattgctgt
     5821 tgtctccccg catgtcccag gtccggttgc tgctacagtt ttagctgtaa acggagcaaa
     5881 tacagttatt tcaactttag gcaatcaatc tgcagaagga aaaacattgt gggaaacggc
     5941 caaacttcaa atgggaataa taggaaacga ttaccggggc agagaagaaa taggaacggc
     6001 cgcatttgaa agagcctcta acaatacatt tatccctgat aattttaact tctggtatcc
     6061 tcaaaggcca cccaatgatc accgcaaccc tgcaaaaaga acattaagcg gacaagtgat
     6121 taaagcaggc caggtcaata tacagggaac atttgctgat tttgatgtca acattgatat
     6181 tgttcctcat cctaaatacg aatatcttgt taccactgca cataaacctg agttcactac
     6241 cattatgaaa ctacaggatg tggcctacga tataaaagat tgcccgccat ccttccatgc
     6301 tgtggaagca gaaatagccg aagattactg gccaaaaggc aatcacactt ttggccgtgc
     6361 aagactaacg gatatggtat taagcagggt gagcagggga atatgtgttt atggcacatg
     6421 gatatacgac atgggtcatt gctgccaccc ggaaattcat ccggcagaac agctctggtg
     6481 gagtgattct tctgaaaaca ggatcaaatc aaacctgaac gttgtttgtg atgcatcccg
     6541 ccggttttgg tggcgcagcc agatggacga tggtaccaaa ctcaaaccct gggctgaacc
     6601 tcccattaaa ggattattcg ccattgcctt tgaatatgca ttacccaatg cagccgctac
     6661 agcttccann nnnnnnnnnn nnnnnaaatt tgaagcagat tatatccagc attacaacct
     6721 cgcagaatac ccaaatgcta atcaaaccta taacctggtg tataatggaa agaacattgt
     6781 ttcctttgta ccgaacaaca atgcttttaa agtctccttt gagcatgtag gtatctcacc
     6841 ggaggacaat aataaaataa gaggattttt ggtacttgaa acttctgtgg gaaagacaac
     6901 acaaattgca acacaagact actatccggg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gaagatgttc
     7021 ttcaaaaaag aagaaggtca ttattatttt acggttacac aaagcataat aaggtctgga
     7081 gctgtttcga caagtgctag ggaaaggcag taaaatattt attacctttt tcacggagca
     7141 attgtatata tatgatttta aagaagaagc aataacatta ctgatgtttt attgcttatc
     7201 agtttctatc ttttcttatt gttcatcact atcattcttt cgaggtcttt ttctgaactg
     7261 ataagtacat cttcattagt gccggtttta ttaaacgtgt cgtatttctc aagtggcttg
     7321 aaattaattc ctgttgcatt agttaatgac ctgatactgc attgatactc tttaaaatga
     7381 gcatttgtat ctatatcaat agcttcatat tgcattccag ctacttcagt aaattttatt
     7441 cctgttatca gttcttcctg tgataattta aaagctgttg ctttaagttg attatcatca
     7501 tttcgccaga caataatctt gaaaaactcc atagggacta tctcacccct gaatttcttt
     7561 ttgtttgaag aaaaaattgg tccgttaaat acagttattc ttccttcact acctttttcc
     7621 tttttgacac ctttctcaag tattgccttt tcgagctttc cccaaattcc gcctccacgg
     7681 ttcaggcctg gaacctgcgg gcaggcattt gtataaaagc atgtatagat accatttcgt
     7741 aaagcagcct cttccgtttt atcccagttg gcatcttcaa atcggctcat atgaccttta
     7801 tcgaaacccg agcctgcata aaacttatct gtaagttgct tacttatatc aatacgttta
     7861 tctcttaacc aatcatcagt tcttttacta ttatccagtc ttaatgccgg attgccttct
     7921 acattaattg cactaagtgc aggcattttc atagtttcat tgaaaagcac actgtattta
     7981 aaatacttca actcgctttt gccattactc atcttggcaa tattcttcat caaagacttt
     8041 tgcggttttg gcaaaggaat atttacaccc ataaaaccag ggtcataacc cttgcatccg
     8101 gaaaaaacta cgtctttctc cttatcagct ttttttgcct cagcaatagt tgtatcaata
     8161 tcgctcaata ttttatccgg tctggcagct acatcatctc ctttattgcg aatactgaca
     8221 gttacttgtc cgctggcttc aagcaaggaa gcaggaaaaa caatactcac atcattattt
     8281 cctgcccaac ccccaatagg atcaggttga ttttttttgt cccagttaac gacaccagat
     8341 ggtaattcta aaggtgaaaa tggtcttctt ttaagttcat ttacataatc agagtgggga
     8401 aagcgactga aaatatcttt caatatgaca cttatacgaa taccttcatt cgcaatccaa
     8461 tgtatttttg tttcatcaac ctccccgttc acaacaggaa taatattacc atctatatcc
     8521 aggaagtttt ttccatctgc actcatactt ggcaccccca tgtggtgcag ggctacaact
     8581 tgccactggt cattaaatac cggacttccg ctgcttccct gtgcagtgtc tgatttatac
     8641 catagtgttg ttggcatgat cttcttaaac tggttttcgc ggattgacaa ctgcttgtaa
     8701 tcaccgccgg ggtggtgtat gatattaagt agttctacat cttcatcccc taatttgcca
     8761 ggagtggggt ctaaaaaaat ataactaata gctgaaagtt gtgcattacc cgaaacatct
     8821 accggttcaa ctgcaactaa gcaataatcc aacgctttta aggaatagaa aaaatcgtct
     8881 ggccttagcc tgaatgaaac aggctgcttg ggtcggccat atatatcata ttcataatta
     8941 aactgcgctt cgctttgcat tacatattct ttttttctga agacatgcca gtttgtaagc
     9001 aataatctag atgaaaccat aaaaccagtg gcaaaaccaa ttgttcgtgg cccctctttg
     9061 ataacaactc tggccacttt ttgctttgta cttgcgataa gctcaacaaa attgctgtaa
     9121 actgaatcgt ttttaccaat tgctctttca aaagcaaaag aagccggttc cggtgctctg
     9181 tcctcaataa gttgcaacct gtttgccagg ttatcattat caccggtgat atttcctttt
     9241 tttactgcct ccggaacatt tttgtcgata tactcttcta actcatcttc aataaattta
     9301 cctttctttt cccctggctt tattctgtct ccagccttaa atcgttcaac tgattgtttt
     9361 atcagctctg ttgaaactaa cataacttta atttatatct ataaataatt ggcctcttaa
     9421 tatcaactgg tattgaaggc atacccagtt aaaattgaga aagaatatcg agatgtgcaa
     9481 taccataaag tggttatttt gaagtatcat tgtttctgct aacttacaat ttgtaacctc
     9541 ttgctaaacc tttccctatg ccctccaaaa tctctccctc cgatttcttg cagcaaagtg
     9601 gcgccaatgc aattgtagaa gaactgcgcc agcattttgt taacagcggt annnnnnnnn
     9661 nnnnnnnnnn gtaaaagata cacagggcaa ccagtatgtg gacctggtgc aggaaggcgg
     9721 cggtgtactg ggtattgcgc tggtcggcta tacttatgtg ctggaaaaaa tgggaatccg
     9781 gtttttcagc atggcaggca ccagtgcagg ggccataaac actatgctgc tggcctgtgc
     9841 cggtaataaa gaagaagaaa aatcgtcgaa gatcgtagag catttggtga agctggaaat
     9901 gttctccttt gtagatggta aatcttccaa ctggaagttc accaaatgga tcaaacgggt
     9961 catacagaag atgctgctgg gcagtaatat gtttaaaaag atcagccgta ttgccactgt
    10021 cattttnnnn nnnnnnnngt tgcttactgt tggttgtttc gtaatcaact tcatttggcc
    10081 cggtgtggcc aaatggatcg ggcttggcgc cggtctttta ttgatatcgg tgattgtctt
    10141 cttttttatc gctgctgcca aatttaaaga gttcgccagg gagggctatg gcctgaatga
    10201 gggaaaggct tttcacaact ggattaaggg agtgatatca tcctttcatg tacgcaatga
    10261 tgtggccatg caaaagatca ataacctggc cgatttctcc taccacttca tgcgggtacc
    10321 caaactggaa gtaataaaag atgatcggag ggagaacaat gatccgccgc atatgccgat
    10381 gcttaccctc atcacctgtg atattatagc caaccgcaag atcgaattta cccagatgtg
    10441 ggacctgtac tggaacaatc aggaagaggt acatccggga gattttgtaa gggccagtat
    10501 gtcaatcccg gtgtttttcg aaacctatac actgagtgat ataagaaaga aaagttcgtt
    10561 tgatatttgg gacaaacacc tgaactggca aaaccagcaa aaacgcatac ccgataaagt
    10621 gcagtttatt gacggaggta ctttatccaa cttccctatc aacgtattct acaatccccg
    10681 gtatccggta ccccgtatgc ccacattcgg cattcgcctg caggatggtg taatggatgc
    10741 cgccagccgt agtaataata gtttattcaa gtatatattt tctttgttca gtaccatccg
    10801 tttcaatttt gaccgtgatt ttatcaccaa aaacagggct tttaactatg gcgtaaagaa
    10861 tattgatgtg caggagcata gctggctgaa cttttttatg aaggacgaag aaaagataat
    10921 cctgtttcgc aaaggagcgc aggctgctgc agaattcctg aaaacatttg actgggcgca
    10981 gtacaaagat gaacggttga agaactttac tatgcaccag gacaggtttg acaatccgaa
    11041 taacctggaa gtatttcctt tctcagaccg taacagttaa aaataaaagg gagtcccccc
    11101 gactcccttt ttatcctgta ttcatcttgg ttattgatta ctctacttat cgttttacca
    11161 ccatttttat agttttttct ttcattcttg aagaggaatg aagcaataaa aggtgaacac
    11221 cttcttccaa ttctgaaagg ttgagacgca cagccggacc gctaacattt tgcctttgca
    11281 aaacagatcc ctgcatatta ataatagcga tacggtcaaa ttcttcttct ttcaggtttg
    11341 tcagaagcaa ttgattttgt acaaatacat aacgaccggt ttcagaaaaa tttgcctgta
    11401 actctgtagt taatggctct tgctggtgtc gtgcctgctt acgcagttgt gcttccactc
    11461 ctgcctgaac cagaaaaaga caaagcagca tgatgagcag ttgttttttc ataaagttgg
    11521 attttagttg ttcgttccgc ctttattaca gcaagaatag tgctgaggtt gacttcaggg
    11581 tactagtacc gtacattttc aggctgaaag aagaagaatc cccaagggag aaaacgacaa
    11641 atcagttcat caccatttcc cgggcattct ccatggcggc ggctgtgggt ttttcgccgc
    11701 ttagcatttt ggcaatggca gtaatccgtt cttctgttgt aagctgacgg atatttgttt
    11761 ttactttatc cctgacgatc tccttgtata caaagaaatg tgcatctgct ttaccggcga
    11821 tctgcggttg atgtgtaata cagataactt gtctttttgc agccagctct ttcatgatga
    11881 taccaacctg tcttgcagct tcgccggaga tgccagtatc tatctcatca aaaataagtg
    11941 ttggcagatc tatagattgc gccaccaatg attttataca cagcatcagg cggctaagtt
    12001 caccaccact tgccactttg cgtaccggct gaaactgccc gctacggttc gcatcaaata
    12061 agaattctat agcgtctgca cctgaactgc taaggtctgc cggctgcact aaaactttca
    12121 gttttgcatt gggcatgcct acctgtgcca gcagcttatt tactttatct tccagaggtt
    12181 tcacctgttt atttcgtccc gctgttattt tctctgctgt cttctttgct tcagcccgta
    12241 agcgatttgt ttctttttct ttttgctgaa tggtttcttc aatatttaaa actgcctgta
    12301 atttttcttc cagttctttc tgtatcgcca ataactcagc ggtagaactt actccatgtt
    12361 ttttctgtag tttatagcct aatgataatc gttcattcag ttgttcaatt ttatccggat
    12421 cataattaat atgattactg atgcggtcta catcatcagc tatatcctgc agctccacct
    12481 gtgctgactg caaacgctga agcaacgctg gcagatcagg atgatacgat gaataaacat
    12541 tcaactggtt caataaaatt tttagttgct gcactaaagg catttcactt tcccgcaggt
    12601 caaaataagt tttggccaaa gcagctttga taccttccga attattgagc agcttaagtt
    12661 ctgcctccgt ttcttccagt tcattttctt taaaagccgc ttcttccaac tcagcaaact
    12721 gaaaacgatt gtaatcggct tctttctcaa attgctgctg ttgtactttg agttcttcag
    12781 tttcctttct caactgcagc cattgccggt attgcacctg gtattcttct atcaatatta
    12841 actggccggc taatgcatcc aatacctgtc gctgaaaatc cgtttcacct aattctaacg
    12901 tatcaaactg ctggtgaagg tcgatcagca aactgcttaa ctgctgcaac tgaaccaggt
    12961 taaccggtgt gtcgtttata aaagcccttg actttccatt tgtaccaatc tcccggcgca
    13021 acaccacttc ttcctctgca tcgagttcat ttgctttgag aaaatccgtt acttcctttt
    13081 tactattccc tgaaaaagtt ccttccacga tgcatttctt ctctttgttc actaaggaag
    13141 aagaatcggc tctttctccc aatatcaaac caagagcgcc gattatgatg gatttaccgg
    13201 cacctgtttc cccggtaatg atgctgagct tgccggaaaa atcaatttcc agttcatcaa
    13261 taatggcata gttatttata gaaagcctcg taagcattga agaggcaaat tagtggttta
    13321 ccacgaatgg cacaaatagc aaagttgctc cgaatagaat caaacaattc gattgcgtga
    13381 aaataaataa gccccgcaaa tgcggggctt attatattaa atagttatag aaagtcttat
    13441 ttcacttcta cattgttgtg caactcctca tctaccagga tgcggccgca gttttcgcaa
    13501 atcatcactt ttttgtgctg gcggatctca ctttgctttt gtgggggaat tgcattaaag
    13561 caaccaccac aggcatctct ttcaacgggc accacggcca aaccattgcg gtagcttttg
    13621 cggatctttt cgtagctggc cagcagacgg gagtctacat gttcccttgc ttccactgcc
    13681 agtttattga aatgcttttc ttctttctcg ttggccacga taattttttc cagttcacct
    13741 ttcttggtag acagtacgcc ttctttaaca gaaatatttt tctttgcctt ttcgagcaaa
    13801 actgcttttt cggctatttc ttctgtagcg tctttgatat gtttttccgc caacttgatc
    13861 tccagctgct gcatttctat ctctttgttg atcgcttcaa actcacggtt gttctttacg
    13921 ttagcacttt gcttctcgta ttttttgatc agggcttcag actctttgat ggcatttttc
    13981 cgctgctcaa taaattcagt aacaccattg atctcttctt cgatgcgggt ctggcgggca
    14041 tgtaaaccag tgatttcatc ttccaggtca gccacttcca tgggcaattc gcctttcagg
    14101 atatggagtt catccagttt actttcaatc ttctgaaggg taacaagtga ggtgagtttt
    14161 tcttctacgg aaaattcttt gacgtgtgcc attgtttatg tttgttttag aggctcagaa
    14221 gtaatgaacc gggttggttc ttacccctgt tttaaggacg gcaaaggtag ggaatttttg
    14281 ctctaaaaac tcctgcagca ggttgatggt gaactgctca ctttcatagt gacccacatc
    14341 agcaataagt agccgtccgt tggcatcaaa aaattcatgg tacttaatat cagctgtaat
    14401 ataggcatca acaccgactg ctaaagcttt ggaaatcaag aagcttccag ctccgccaca
    14461 gatagctact ttttttacag ccttattaag ataagccgtg tggcgtacca ccggcacacc
    14521 aaaaaccctt ttgatccgtg ccaggaattt cttttcatcc agtgaggcag gcaattcgcc
    14581 gataactccc gaaccaaagc cctggtattt gttggaaaga ggaaaaatgt cgtaggccac
    14641 ctcttcatag gtatgcgcag ccttcataac cgaaaccagc cggctttcaa gccaggccgg
    14701 aaagatgact tcgatcttta cttcctgctc tttatgacgc tttcctct
//
LOCUS       DAORLS010000038        14628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2208_length_14628_cov_2.042133, whole genome shotgun sequence.
ACCESSION   DAORLS010000038 DAORLS010000000
VERSION     DAORLS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2208_length_14628_cov_2.042133"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>254
                     /locus_tag="PK977_04720"
     CDS             <1..>254
                     /locus_tag="PK977_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17444.1"
                     /translation="GKNVLDLSVPVELPYRIAGQDREVIITDTLPIKRHGDFRRFLKD
                     RRIEDLLEYLSADRAKISRPELEVEIETYDQQREAFFERI"
     assembly_gap    255..405
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(492..806)
                     /locus_tag="PK977_04725"
     CDS             complement(492..806)
                     /locus_tag="PK977_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRF17445.1"
                     /translation="MNVIFQRLGLNMIDGGMVSISSVKVTPDLLEARFYLSFFQVKDV
                     KAALHKIEDRHHDIKKELAAHVRHQLRSIPVLKFFHDDVVRAVGQFKQPFGNLFAIVR
                     IN"
     gene            949..2751
                     /locus_tag="PK977_04730"
     CDS             949..2751
                     /locus_tag="PK977_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF17446.1"
                     /translation="MKQYARIFRYLKAYKGKIALYFLFIVLSIIFSIVSVGMLMPFLQ
                     LIFTGEGTFKATNNPAIQFFNNMLLDSLKDPNNKVPVLGYICILMISFIIMKNLFLYL
                     SYYILNPLKNTIVNELRQDLYDKVLKLPIGFFTEKRKGDLMSRMTNDVGEVEGSVVGT
                     LEGWIRDPLTIIINLTVLFLISPQLTLFILILIPVLGLVIGRVTKSLKRHSEEAAKKS
                     SDALSTLEETLGGLRVIKAFNVERLMRSRFRKNNEELLHAKNHISYRRDMASPLSEVM
                     GVMLFTIVLYYGGRLVLQNELLEASVFLGYLAIFYNIINPAKALSTSFSNMRKGAAAI
                     GRIEEVLTAPITVDDNPNGKQLTEFRDKIEFRNVRFAYDDAVILDDINLTVQKGKTIA
                     LVGSSGAGKSTLADLVPRFHDVTSGEVLIDGVNIRDYSLHSVRSLMSIVTQEPILFND
                     TIAHNIALGHQEATHDQIVEAAKIANAHEFITKKENGYNYNIGDRGSKLSGGERQRLT
                     IARAVLKNPPILILDEATSSLDTESERLVQDAINNMMMNRTSIVIAHRLSTIRHADEI
                     IVLQKGKIVERGTHEQLLSQGGFYKKLVEMQEVR"
     gene            2944..3435
                     /locus_tag="PK977_04735"
     CDS             2944..3435
                     /locus_tag="PK977_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRF17447.1"
                     /translation="MAENNKTFKVKANDFVFSFDQATIDNSDFYKKSPDEFNIIKDNR
                     SANAKLLESDISGKKLKIEVEGEAFDIEIKDELDQRLDSMGFSNISTKHIKEIKAPMP
                     GLVIDVTVKDGQEVKEGDRLLILEAMKMENSIMIHANATIKRVAVTPGQAVDKGQVLV
                     ELA"
     gene            3456..>3657
                     /locus_tag="PK977_04740"
     CDS             3456..>3657
                     /locus_tag="PK977_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRF17448.1"
                     /translation="MKKILVANRGEIALRIMRTIRKMGIKSVAVYSEADRHSPHVLFA
                     DEAVCLGPAPSSQSYLNGEKIIA"
     assembly_gap    3658..3778
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3779..>4042
                     /locus_tag="PK977_04745"
     CDS             <3779..>4042
                     /locus_tag="PK977_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF17449.1"
                     /translation="RIMGSKLAAKESVKKYNIPMVPGIDRAIEDVNLAIEVADKIGYP
                     VLIKASAGGGGKGMRIVEKADDMQEQMERAISEAKSSFGDGSV"
     assembly_gap    4043..4220
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4221..4929
                     /locus_tag="PK977_04750"
     CDS             <4221..4929
                     /locus_tag="PK977_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HRF17450.1"
                     /translation="AAVLVAKSCNYVSTGTVEFLVDDKLNFYFLEMNTRLQVEHPVSE
                     MIIGLDLVEQQIRIARGEKLAVTQDDIKPNGHAIELRVYAEDPLNNFLPSIGTLTKYE
                     KPVGEGIRVDDGYEEGMTIPIYYDPMIAKLVVHGKTRTEAIQKMLEAIKNYKIEGVST
                     TLPFGTFVFEHDAFFSGNFDTHFVKNYYTPEKIKEKQKANAEIAALAALKYWQEKKKM
                     LNPVEHKSTSWKRRLAH"
     gene            4962..6503
                     /locus_tag="PK977_04755"
     CDS             4962..6503
                     /locus_tag="PK977_04755"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRF17451.1"
                     /translation="MKSKVEILREKLEEGKLGGVIKRIEAQHKKGKLTARERVELLMD
                     PGSFEEIGALVTHRTKEFGMEDQKYYGDGVITGYGTVNGRLVYVFAQDFTVFGGALSE
                     THAEKICRIMDMAMKSGAPMVGLNDSGGARIQEGVRSLGGYADIFFRNVQASGVIPQI
                     SAIMGPCAGGAVYSPAMTDFTIMVENTSYMFVTGPNVVKTVTNEEVSSEELGGASTHS
                     TKSGVTHLTALNDMDCINQVKKMLSYMPQNCEDVVQKIPYTLGDETREVLETIVPASA
                     NQPYDIREVIKGICDTDSFFEIHEYYADNIVVGFARLAGRSIGIVANQPQSLAGVLDI
                     ESSKKGARFTRFCDCFNIPLLVLVDVPGFLPGTDQEWNGIITNGAKLLYALSEATVPR
                     CTVITRKAYGGAYDVMNSKHIGADMNYAWPTAEIAVMGAKGASEIIFKNEIAAAEDPA
                     KKLLEKEAEYAELFANPYTAAERGFIDEVIEPKQTRKKLIKAFAMLENKVAKRPRKKH
                     GNIPL"
     gene            complement(6574..7716)
                     /locus_tag="PK977_04760"
     CDS             complement(6574..7716)
                     /locus_tag="PK977_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF17452.1"
                     /translation="MNFQQSELTQQVAQTARDFAQQHIKPYLMEWDESQEFPVHVFKE
                     MGKLGMMGVLVPHDYGGAGLSYFEYKVVIEEIAKVCGSVGLSLAAHNSLCTGHILNFG
                     NEEQKKKLLPKLATAEWIGAWGLTEANTGSDAGNMKCTAVKEGNEWVINGTKNWITHG
                     KSGEIAVVICRTGEPRAKNNSTAFVVERGTPGFSAGKKENKLGMRASETAEMVFDNCR
                     IPDANRLGEVGDGFKQAMKVLDGGRISIASLSLGIAKGAYEAALKYSQERYQFDQPIA
                     SFQGVSFKLADMATEIAAAELLTLQACDLKNRHLPMTKEAAMAKYYASEVAVKVANDA
                     VQVFGGYGYTKDFPVEKHYRDAKLCTIGEGTSEIQKIVISREVLRQ"
     gene            complement(7872..8651)
                     /locus_tag="PK977_04765"
     CDS             complement(7872..8651)
                     /locus_tag="PK977_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF17453.1"
                     /translation="MNCPPLKITFLGTGTSSGVPMIGCDCAVCISTDKKDKRLRSSIL
                     VQSAKTALVVDTGPDFRYQMLRQKVKHLDAVVFTHPHKDHLAGLDDVRAFNFFSKKHI
                     DIYADSLTEEAVRRDFYYAFTDTKYPGIPELNLHTISDEPFVVGDIPIIPVQVWHLKM
                     PVLGFRFGKFTYITDANRIDDIEKDKIRNSEVLVLNALRKQKHISHFTLDEAMEMAAE
                     VKAGTAYFTHLSHQMGKHGEIEAELPDNIHLAYDGLELTFS"
     gene            complement(8694..8975)
                     /locus_tag="PK977_04770"
     CDS             complement(8694..8975)
                     /locus_tag="PK977_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17454.1"
                     /translation="MKYTQTTLNKLEAIPEEAGYVLRYERGTFQSGYCILEQKKVVVL
                     NKFLQFEGRINTLVDLIPQLEIDSTLLTEESQKLYGEIMAKLETEGKEE"
     gene            complement(9096..10652)
                     /locus_tag="PK977_04775"
     CDS             complement(9096..10652)
                     /locus_tag="PK977_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HRF17455.1"
                     /translation="MAVAKIIWVDDEIESLQSQKIFLENKGYEVQTFTNGFDAIDFVK
                     ENPVDVVLIDESMPGITGLETLAKIKEVNQSLPVVLITKNETENLMDEAIGSQISDYL
                     IKPVNPNQVWLSLKKIIDNKRLVAEKTTTAYQQQFRNLFMALNSNPDYNEWMDIYKKL
                     VYWELEMQKSDSPEMQEVFQSQKNEANTEFFKFISRNYASWVSPKSTDGPVMSHTLMK
                     FKVMPHLEKGTPLFFILIDNLRFDQWKAIQPIFAESFRILEEESFYSILPTATQYARN
                     AIFSGLMPVDIEKQFPVQWKNDDEEGGKNLHEEELFRAHLKRLGKADLKVSYTKVLNN
                     QAGLDLVNNIHNLLNNDLNVIVYNFVDMLSHARTEMEVLKELAGDEMSYRSITASWFE
                     HSPLHQALKKIADKNIKIVLATDHGSVRVKTPCKVVGDKQTTTNLRYKHGRNLNYDPK
                     DVLAFRDPREAGLPVPTINSSFIFAKEDNFLCYPNNYNYYVNYYRNTFQHGGVSLEEM
                     IVPVIKMQSK"
     assembly_gap    10913..11037
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11038..>11291
                     /locus_tag="PK977_04780"
     CDS             <11038..>11291
                     /locus_tag="PK977_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF17456.1"
                     /translation="ITGEEELGAKVAILLHDIGHGPFSHALEHELIKDVHHEEISILL
                     MHKLNDEFKGQLNTAINIFTDKYPKKFLHQLVSGQLDVDR"
     assembly_gap    11292..11511
                     /estimated_length=220
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11512..12021
                     /locus_tag="PK977_04785"
     CDS             <11512..12021
                     /locus_tag="PK977_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRF17457.1"
                     /translation="RAKELLADEKRVDAASAELDFFLKNFNNQGPIEQHLDTFCRLDD
                     HDVMATIKNWCHHPDKILSTLCRSLVERKLLKVKLQSAAFDLGMVKEKTRETCTALGI
                     GEKEAGYFVFTGIASNTTYDPGDERINILFKDGTIKDISAVDNALISVKSGLAYGEVK
                     KHYICYYRC"
     gene            12082..13125
                     /gene="lpxD"
                     /locus_tag="PK977_04790"
     CDS             12082..13125
                     /gene="lpxD"
                     /locus_tag="PK977_04790"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRF17458.1"
                     /translation="MTFPAAQIAFLINGKVEGDAQVAVNSFGKIEEATEGQLAFLANP
                     KYEEYLYSTKASVIIVNETQELRQPIKATLIRVPDAYSAFATLLSKYQEIAQQQLTGV
                     QEPCYISKSAKYGENVFIGAFAYLGENVKVGNNTKIYPNVYLGNNVVIGDNTIIHPGV
                     RIYHDCRVGNHVTIHAGTVIGSDGFGFAPQADGSFKKVPQIGNVVIEDNVEIGANATI
                     DRATIGSTLIKSGAKLDNLIQIAHNVEVGHSTVIAAQAGVSGSTKIGNGVMIGGQAGI
                     VGHIQLGDGSKVNAQSGVSKSLEPGKAVTGSPAYDYTSSLRSQAIFRNLPEMEKRLKE
                     LEALVKQLMAERV"
     gene            complement(13206..13787)
                     /locus_tag="PK977_04795"
     CDS             complement(13206..13787)
                     /locus_tag="PK977_04795"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF17459.1"
                     /translation="MKKYFLPWLLLAVTIVQAQPWTKQYDFVDDCVCGMAKVSKDGKF
                     GYVDQQGKLIVPVIYQDAMAFSEGFAAVKSENKWGYLDSTGKMVINPEYEEAGSFHES
                     LAAVRKNGKFGFVDKNNKVIIPFNYSMANGFSEGMAPVANDKDMWGYVNASGKLVIPC
                     VFTFADKFEDGRARVIKGSEMSYIDRYGKAVKD"
     gene            14029..>14628
                     /locus_tag="PK977_04800"
     CDS             14029..>14628
                     /locus_tag="PK977_04800"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRF17460.1"
                     /translation="MNPLTDKMPFTDKNNLASFNPDKQHTLKSSVSISGTGLHTGIMA
                     DLTLKPASPGFGFQFQRIDLPNQPIIKADCDLVTDVSRGTTLEANGAKVSTVEHVLAA
                     LVGMGIDNCLLEINGPEMPIMDGSSQPFIELIEEAGIVEQDAAKVWYSIDENIYHYDE
                     SKRVEMVAMPATEYQVTTLIDFNSPVLGTQHAGLKHISEF"
BASE COUNT         4084 a         2980 c         2895 g         3874 t
ORIGIN      
        1 cggtaaaaac gtgcttgatt tgtccgtgcc ggtggaattg ccctaccgca ttgcgggtca
       61 ggatcgggag gtgatcatta ccgacacgtt gcccatcaaa cggcacgggg atttccggcg
      121 ttttctcaag gatcgccgca tcgaagacct gctggaatac ctgagcgccg atcgcgcaaa
      181 aatttccaga cctgaactgg aagtcgaaat tgagacatac gaccagcaac gcgaggcatt
      241 ttttgaaaga atttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgac ccgctcggtt
      421 tggagaaaac ttgcgaactg cgcaacaaac tttgccacaa tgaaatacct tcggctgcga
      481 atttttcggc tttaattgat ccggacgata gcaaacagat tgccgaacgg ttgtttgaac
      541 tggccaaccg cacgtacgac atcatcatgg aaaaacttca ataccggtat gctgcgcaac
      601 tggtgcctta catgggcagc cagctccttt ttgatgtcat ggtgcctgtc ttcaatctta
      661 tgcaaggctg ctttcacatc tttcacctgg aaaaaactca ggtaaaaacg ggcctccagc
      721 aggtcgggtg tcacttttac tgatgatatg gaaaccatac ctccgtcaat catgtttaat
      781 cctaatcgct gaaatatgac attcatctcc tcgttcagta aacctgcaat ctgtttttgt
      841 ctttttcctt ccaccatagt tttgtcatta ttttccgtct gcacattcag gcagtgtcag
      901 acgctgtaaa attagttact ttgcggcttt gcaagggaag cattttcaat gaaacaatac
      961 gccaggatat tcaggtattt gaaagcatat aaggggaaaa ttgcccttta tttccttttt
     1021 attgtattaa gcataatttt ttccattgtt tcagtgggta tgctcatgcc ttttttacag
     1081 ttaatcttta ctggtgaagg aacttttaaa gcgaccaata acccggccat tcagtttttc
     1141 aacaatatgc tgcttgattc actgaaagac cccaacaaca aagttccggt acttggttat
     1201 atctgcattt tgatgatctc tttcatcatt atgaagaact tatttcttta tctctcttat
     1261 tatatcctta accccttaaa aaataccatt gtcaacgaac tgcgccagga tctttatgat
     1321 aaagtactga agctgccaat cggctttttt acagaaaaaa gaaaaggaga cctgatgagc
     1381 cgaatgacca acgacgtggg tgaagtagaa ggctcggttg tgggcacatt ggaaggctgg
     1441 atccgtgacc cgctaacaat catcattaac ctgactgtac tcttcctgat aagtccgcaa
     1501 ctaacccttt ttatattaat actcattccg gtattaggcc tggttatagg ccgtgttacc
     1561 aaatcactta aaagacattc agaagaagca gcaaaaaagt caagcgatgc cctatcaacc
     1621 ctggaagaaa cgctgggagg ccttcgtgtt ataaaagcgt ttaatgttga aagactgatg
     1681 cgatcgaggt tcagaaaaaa caatgaggaa cttttacatg caaaaaatca catttcctat
     1741 agaagagaca tggcatctcc tctctctgaa gtgatggggg taatgctttt taccattgtg
     1801 ctgtattatg gcggcaggct tgttctacag aatgaactgt tggaggcctc cgtattcctg
     1861 gggtatcttg ctatttttta taacatcatt aatcctgcta aagccttatc tacatcattc
     1921 agcaatatgc gaaaaggcgc tgctgccatc ggacggatag aagaagtgct caccgcacct
     1981 ataacagtag atgacaatcc aaacggaaag caacttactg aattcaggga taagattgaa
     2041 tttcgtaatg tacgttttgc ctatgatgac gcagtgattc ttgatgatat taaccttact
     2101 gtacaaaaag gaaaaaccat tgcattggtt ggctcgtcag gagctggtaa atcaacgcta
     2161 gccgacctgg taccccgatt tcatgatgta acatcgggtg aagtattaat agatggagtt
     2221 aatatcaggg actactcatt acattctgtt cgaagcctga tgagtattgt tacccaggag
     2281 ccaatccttt ttaatgatac gattgcccat aatatcgcat taggccacca ggaagccaca
     2341 catgatcaga tagtggaggc ggctaaaatt gccaatgccc atgagtttat cactaaaaaa
     2401 gaaaatggct ataactacaa tataggcgac cggggcagca aactgagcgg aggagaaaga
     2461 cagcggttaa cgattgcaag agctgtttta aaaaacccac ccatactgat attggatgaa
     2521 gctacttctt ctcttgacac agaaagtgaa cggttggtac aggacgctat aaataatatg
     2581 atgatgaacc gaaccagtat tgtgatagct caccgtcttt ccaccataag acatgcggat
     2641 gagatcatcg tcttacaaaa aggcaaaatt gtagaaagag gaacccatga acagctactt
     2701 tcacagggtg gtttctataa aaagctggta gagatgcagg aagtaagata gtcccctcta
     2761 ttcctgattt taattctccc ctttcttgag tataatcatt ccgaatctgg cgtctattga
     2821 caaccttgca gtttgaatta ccgggtagca ataccattta aactatccct tcggggaaag
     2881 cggacatgaa acatctttcg atatcttgcg aatcaactaa ctttcaagta aatcccattc
     2941 agtatggcag aaaacaataa gaccttcaag gttaaagcca atgactttgt cttttctttt
     3001 gaccaggcta cgatagacaa ttctgacttc tacaaaaaat cgccggatga atttaatatc
     3061 ataaaagata accggtccgc caatgcaaag ttgctggaat cagacatttc cggtaaaaaa
     3121 ctcaaaattg aagtagaagg cgaagccttt gatattgaaa taaaagacga actggaccag
     3181 cggcttgaca gcatgggctt cagtaatata tccactaaac atatcaagga aataaaagcg
     3241 ccgatgcccg gtcttgtaat agatgtaact gtaaaagatg gacaagaagt aaaagaaggg
     3301 gaccggctgc tgatactgga agccatgaaa atggaaaaca gcatcatgat acatgccaat
     3361 gccactatca aaagggtggc cgttacccct ggccaggctg ttgacaaagg ccaggtttta
     3421 gtagagttag cttaatcaaa aacaaaatca tcgagatgaa aaaaatatta gttgccaacc
     3481 gtggtgaaat agctctccgc attatgcgta ccatccgaaa aatggggatt aaatcggttg
     3541 ctgtttattc agaagcggat cgtcattcac cgcatgtatt atttgcagac gaagcagttt
     3601 gcctgggacc ggcaccatcc agtcaatctt acctgaatgg tgagaagata attgccannn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     3781 gcattatggg ttcaaaactg gctgctaaag agagtgtgaa gaaatacaat ataccaatgg
     3841 tgcccggaat tgacagggct atcgaagatg taaacctcgc cattgaagtt gctgataaaa
     3901 ttggataccc tgttttgatc aaagcgtctg ccggaggtgg cggtaagggt atgcgaatag
     3961 ttgaaaaggc agatgatatg caggaacaga tggaaagagc tattagtgaa gcaaaatcat
     4021 cttttggtga cgggtctgtt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn agcggcggta ctcgtagcca aatcctgcaa ttatgtaagt
     4261 acaggcacag tggaattttt agtagatgat aaactaaatt tctacttttt ggaaatgaat
     4321 acccggttgc aggttgaaca cccggtttcc gaaatgatca ttggtcttga cctggtagaa
     4381 caacaaataa ggatcgcaag aggtgaaaaa ctggcggtta cacaagatga tatcaaaccc
     4441 aatggccatg caattgaact cagagtgtat gctgaagacc cgctgaacaa tttccttccc
     4501 tcgatcggga cattaaccaa atatgaaaag cctgttggtg agggcatccg ggtggatgat
     4561 ggctatgaag aaggaatgac catcccaatt tattatgacc cgatgatcgc caaactggtg
     4621 gtgcatggca aaacaagaac ggaagcaatt cagaaaatgc tggaggcaat taaaaattat
     4681 aagatagaag gtgtgtccac cacattgcct tttggaacat ttgttttcga acatgatgct
     4741 ttcttcagtg gcaactttga tacacatttt gtaaaaaatt actatacccc tgaaaagata
     4801 aaagaaaaac aaaaggccaa tgcagagatt gcagcactgg cagcattaaa gtactggcag
     4861 gagaaaaaga aaatgctcaa tccggttgaa cataaatcca ccagctggaa aagaaggctg
     4921 gcacattaat catcaattga ccattcacaa caacatttat tatgaagtca aaagtcgaaa
     4981 tactacggga aaaattagaa gaaggaaaat tgggcggtgt cataaaaaga attgaagcgc
     5041 agcacaaaaa aggaaaactt acggcaagag aaagggttga actgctcatg gaccccggct
     5101 catttgaaga aatcggggcg ttggtaacac accgaacaaa ggagtttggt atggaagacc
     5161 agaaatatta tggagatggt gttattaccg ggtacggaac tgtgaacgga agacttgtat
     5221 atgtttttgc ccaggatttt actgtttttg gcggtgcctt atctgaaaca catgccgaaa
     5281 agatctgccg cattatggat atggccatga aatctggtgc ccccatggta ggattaaatg
     5341 actcaggtgg tgcccgcata caagaaggtg tacgatcact gggcggctac gctgatatat
     5401 tttttaggaa tgtgcaggcc tctggtgtaa taccacagat ttctgccatc atggggccct
     5461 gtgcaggtgg cgcagtatat tctcctgcaa tgactgattt taccattatg gtcgagaaca
     5521 ccagttacat gtttgtaacc ggtccaaacg tagtaaaaac ggtaaccaac gaagaagtaa
     5581 gctcagaaga actgggaggt gcatctactc actctactaa gtcaggagta acacacctta
     5641 cagcattgaa tgatatggac tgcatcaacc aggtaaaaaa aatgctgagt tatatgccgc
     5701 aaaactgcga ggatgtagtg cagaaaattc cttacacttt gggagatgaa acaagagagg
     5761 tgctggaaac aattgtacct gccagcgcca atcagcctta tgatatccgg gaagtgataa
     5821 aagggatttg tgatacagat agtttttttg aaatccatga atattatgca gataatattg
     5881 tggtaggctt tgcccggctt gccggaagaa gtatcggtat cgttgccaat cagccgcaaa
     5941 gtcttgccgg ggtactggat attgaaagct cgaagaaagg agcaagattc acccggttct
     6001 gcgactgttt caatattccc ttattggtat tggtggatgt accaggcttt ttacccggca
     6061 ctgaccagga atggaatggc atcatcacca atggagcaaa actcctgtat gcattaagcg
     6121 aagctactgt tccccgttgt actgttatta cccgaaaagc ttatggaggc gcttacgatg
     6181 taatgaactc caaacacatt ggcgccgaca tgaactacgc atggcctact gctgaaatcg
     6241 ctgtgatggg tgccaaagga gccagtgaga tcattttcaa aaatgaaatt gctgcagccg
     6301 aagacccggc taaaaaactg ctggaaaaag aggctgaata tgctgaactc tttgcaaacc
     6361 cttacactgc tgcagaaaga ggatttattg atgaagtgat agaaccaaaa caaacaagga
     6421 agaaactcat caaggctttt gctatgctgg aaaacaaagt agccaagcgg ccaaggaaaa
     6481 aacacggaaa tattccctta taataatttg ttgtgcttaa ataatgaggc cccggtaaac
     6541 tggggccttt caattattag tgatttttaa aaattattgc cgcaacactt cacggctaat
     6601 aacgatcttt tgaatctcgc tggtaccctc tcctatcgta cacaacttgg catcccggta
     6661 atgtttttct acaggaaaat ctttggtgta gccatatcct ccaaaaacct gtacagcatc
     6721 gttggctact tttacggcta cttcgctggc gtaatacttt gccatggctg cttcttttgt
     6781 cataggtaaa tggcgattct tcagatcaca ggcctgcagg gtcaatagtt cagcagcagc
     6841 aatttctgta gccatatcag ccagtttaaa agaaactccc tggaaactgg cgatgggctg
     6901 atcaaactgg tagcgttcct gggagtattt caatgcagct tcataagctc ctttagcaat
     6961 acccaacgat aatgaggcaa tggatattct gccgccatcg agcactttca tggcttgttt
     7021 aaaaccatca cctacttcgc caagccggtt agcatccgga atacggcagt tatcaaacac
     7081 catctctgct gtttcactgg cccgcatacc cagcttattt tctttttttc ctgcgctaaa
     7141 tcccggtgtt cctctctcca ctacaaaagc ggtggaatta ttcttcgctc tcggttctcc
     7201 ggtacgacaa ataacaacag caatctcccc gcttttgccg tgggtgatcc agttcttcgt
     7261 cccgtttatc acccattcat ttccttcttt cactgctgta catttcatat taccagcatc
     7321 actgcctgta ttagcctctg ttaagcccca tgcgccaatc cattctgccg tggccagttt
     7381 aggcaacaac tttttctttt gctcttcatt accaaaattg agaatatgac cggtgcagag
     7441 tgaattatgt gctgcaagac ttaaaccaac agagccgcat actttggcaa tctcttcaat
     7501 caccacttta tactcgaaat aggatagccc tgcgccccca taatcatggg gaaccagtac
     7561 acccatcatt cccaatttcc ccatttcttt aaaaacatgt acagggaatt cctggctttc
     7621 atcccattcc atcaaatagg gcttaatatg ttgttgtgca aaatcacggg ccgtttgagc
     7681 cacttgttga gtcagttcag actgttgaaa attcatacat tcaatcgttg gttattgcga
     7741 aataactgtg ccactccttt cgggggtggc acagtcttgg gcctccaggg gaatacaagc
     7801 aatcattaga ggcagtgcaa aataaaggaa aatttttaaa aactaacaga tgttagtatg
     7861 aaaatctgct attaactaaa cgtcagttcc agcccatcat aagcaaggtg gatattatca
     7921 ggaagttctg cttcaatctc gccatgctta cccatttggt gagataaatg agtaaaatag
     7981 gctgtccccg cttttacctc tgcagccatt tccatggctt catccaaagt aaaatgagag
     8041 atatgttttt gcttacgcag ggcatttagt acaagtacct cgctgttacg gattttatcc
     8101 ttttcaatgt catctatccg gttagcatca gtgatatagg tgaacttacc aaacctgaaa
     8161 cccagcaccg gcatttttaa atgccagact tgtacaggta taataggaat atctccaacc
     8221 acaaacggct cgtcggaaat agtatgaaga tttagttccg gtatacccgg gtattttgta
     8281 tcggtgaaag cataatagaa atcccgtctc acagcttctt cagtcaaaga atcagcgtaa
     8341 atatcaatat gctttttaga gaagaaatta aatgccctga catcgtccaa tcctgccagg
     8401 tgatccttat gcggatgcgt gaatacaact gcatccaggt gttttacttt ttgcctgagc
     8461 atctggtaac ggaaatccgg gcctgtgtca accaccaaag cggttttggc agactgcacc
     8521 agtatgcttg atcgtaaacg cttatccttt ttatctgtgg aaatacagac ggcacaatcg
     8581 cagccgatca tagggactcc actgcttgtt ccggtaccga gaaaggtaat tttgagaggt
     8641 gggcagttca catggcaaag ttagcaggtt aggagtttgc attgcctttc tactcactcc
     8701 tccttaccct ctgtctccag tttggccatt atctcaccat agagtttttg agattcttct
     8761 gtaagcaggg tgctatctat ttccagctgt ggaatgagat caaccagggt attgattcgc
     8821 ccttcaaatt gaagaaattt atttaatacc actacctttt tctgctccag tatgcagtag
     8881 ccgctctgaa aggtacccct ttcatacctg agcacatagc ctgcttcttc cggaatggcc
     8941 tctaatttat tgagagttgt ttgcgtatac ttcattttac caccatcccc taaagggggg
     9001 ttatgggtaa ttgcaaattt atttgctacc tgctaatcgt tactgaagat caaacaagtt
     9061 ttttccacaa aaagctcccc ttattaagat agagatcact tgctctgcat tttaataact
     9121 ggcacaatca tttcttccag actaaccccg ccgtgctgaa aagtgttgcg ataataattt
     9181 acatagtaat tatagttatt gggatagcaa aggaagttat cctcttttgc aaaaatgaaa
     9241 gaagaattga tggttggtac cggcaaccct gcttctcttg gatcccggaa ggccagtaca
     9301 tctttcggat cataattaag gttacggcca tgtttatagc gcaaattggt ggttgtttgc
     9361 ttgtcaccaa ccactttgca aggcgttttt acccttacac taccatgatc agtagccaaa
     9421 acgattttga tattcttgtc tgcaattttt ttcaacgcct ggtgaagcgg agaatgttca
     9481 aaccaactgg ctgttatact ccggtagctc atttcatcac ctgccagctc tttcagcact
     9541 tccatttctg ttctcgcatg gctgagcata tccacgaaat tgtaaacaat tacattcaaa
     9601 tcattattga gcaagttgtg gatattgttc accaggtcta gccccgcctg gttatttaaa
     9661 accttcgtat aggaaacctt caggtccgcc ttgcccaatc gcttcaggtg cgccctgaat
     9721 agctcctctt cgtgtaaatt tttcccgcct tcttcatcat cattcttcca ctgaaccggg
     9781 aattgttttt caatatcaac cggcatgagt cctgaaaaaa tggcgtttct ggcatattga
     9841 gtagcagtag gcaatatact gtaaaagctt tcttcttcca gtatccgaaa actctcagca
     9901 aaaatgggct gaatagcttt ccactggtcg aaacgcaggt tatcaatcag aataaaaaaa
     9961 agaggagtac ctttttccag gtgtggcatt accttgaatt tcatcaatgt atgactcatc
    10021 accggaccat ccgtactttt tggtgatacc cagctggcat aattacggga aatgaatttg
    10081 aaaaattctg tattggcttc gttcttctga ctttgaaata cttcctgcat ctccgggctg
    10141 tcactttttt gcatctccag ttcccaatac accagttttt tataaatatc catccactcg
    10201 ttatagtccg ggttactgtt caacgccatg aagagattac ggaattgttg ctggtaggcc
    10261 gtagttgttt tttctgctac cagacgttta ttatcaatga tcttcttcaa actgagccac
    10321 acctggttgg ggttaaccgg tttgatcaaa taatcactaa tctgcgaacc aatagcttca
    10381 tccatcaggt tctccgtttc atttttggtt atcagcacta ccggcaacga ttggttaact
    10441 tcttttatct tggcaagcgt ttccagcccg gtaatgccgg gcattgactc atcgatcaaa
    10501 actacatcta ccgggttttc ctttacaaaa tctatggcat cgaatccgtt ggtaaatgtt
    10561 tgtacttcgt aacccttatt ttctaagaat attttttgcg attgcagact ttcaatctcg
    10621 tcgtctaccc aaatgatttt tgctactgcc atcctgtata tttaacgatt ttagatttat
    10681 gactgcggat tcttatccat tgaaaaaacc ggcgccgctc aaatttagtt taataagccc
    10741 agctattgta gctttacggc cttgtgttta acaaaaaagc aacaatgaca gctgcccgta
    10801 agatcattaa tgaccctgtt tacggtttta ttacaattga tcaaccgttg atattgcaga
    10861 ttatttcaca tccttattac cagcggctgc gaaatattca ccagatggca ttnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
    11041 tacgggtgaa gaagaattag gagctaaagt agcgatactg ctgcatgata ttggtcacgg
    11101 accattctct catgccttag agcatgaact gataaaagat gtacaccatg aagaaatttc
    11161 aattctgctc atgcacaaac tgaatgatga gtttaaagga caattgaata cagctatcaa
    11221 tatttttaca gataagtatc ccaaaaaatt tttgcatcag ctggtgtctg gccaactgga
    11281 tgtggaccgg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgggctaaa
    11521 gaactgttgg cggatgagaa aagagtggat gctgcatcag cagaactgga tttctttctt
    11581 aaaaatttca ataaccaggg gcctattgag cagcatttag atacgttctg ccgtcttgac
    11641 gaccatgatg taatggccac catcaaaaac tggtgccatc acccggataa aatattatca
    11701 acgctttgcc gcagccttgt ggaacgaaag ttgctgaaag taaaattgca gtcagctgca
    11761 tttgacctgg gtatggtaaa agaaaagaca agggaaacct gtacagcatt aggaatcgga
    11821 gaaaaagaag caggctattt tgtatttacg ggtattgcca gcaataccac ttatgacccg
    11881 ggcgatgaaa ggatcaatat tttatttaaa gatggaacaa taaaagatat ttctgccgtt
    11941 gataatgcat taatcagcgt taaaagcggt ctcgcctatg gtgaggtcaa aaaacattac
    12001 atttgttatt atcgttgtta gttgccggta tttccttcgg ttaaataaag agccaggaac
    12061 aaacaacccg aaaccagtaa tatgacattc cctgctgcac aaatagcatt tctcatcaat
    12121 ggaaaagtgg aaggtgatgc ccaggtggct gttaattcct ttggcaaaat tgaagaggcc
    12181 accgaaggac aactggcctt tttggctaac cccaaatatg aagagtacct gtacagtact
    12241 aaggcttccg ttattattgt aaatgaaaca caggagttaa ggcagccaat aaaagcaaca
    12301 ctgattcgtg tacctgatgc ctattctgcc tttgctacgc ttcttagcaa ataccaggaa
    12361 atagctcagc agcaattgac aggcgtacag gagccctgct atatttctaa atcagcaaaa
    12421 tatggtgaga atgtttttat cggcgcattt gcttacctcg gtgaaaatgt aaaagtgggg
    12481 aataacacca agatctatcc taatgtatat ttaggcaata acgtagtaat tggcgataat
    12541 actatcattc atccgggggt tcggatatat catgattgca gggtgggaaa tcatgtcacc
    12601 attcatgcag gtacagtgat tggcagtgat ggttttggtt ttgccccgca ggctgacgga
    12661 agtttcaaaa aagtaccaca aattggcaat gttgtaattg aagacaatgt agaaatcggt
    12721 gccaatgcta cgattgacag ggccaccatc ggttctaccc ttattaaaag cggagccaaa
    12781 ttagataacc ttatacaaat tgcccataat gtggaagtgg gccatagcac agtaatagct
    12841 gcccaggcgg gggtaagcgg cagtaccaaa ataggcaatg gcgtaatgat tggcggacag
    12901 gcgggtatcg ttggccatat tcaattaggc gatggctcca aagtaaacgc acaaagtggt
    12961 gtgagcaaat ctctcgaacc cggcaaggcc gtcaccggct cgccggctta tgactatact
    13021 tcctctcttc gcagccaggc tatttttcgc aatctgccgg aaatggaaaa aagattgaag
    13081 gaactggaag ctcttgtaaa acaattaatg gccgaaagag tgtagagata atatctcact
    13141 ctccggccat tggtttatgc aatagctact aactgaataa gcttcctaat atttcgacag
    13201 ttcttttaat ctttcaccgc tttaccatag cgatcgatat agctcatctc actgcctttt
    13261 atcacccttg cccttccatc ctcaaatttg tctgcaaaag tgaatacaca aggtattacc
    13321 agtttaccac tggcatttac atagccccac atgtctttat cgtttgctac aggtgccata
    13381 ccttctgaaa atccattggc cattgagtaa ttgaaaggaa taatgacctt gttgttttta
    13441 tctacaaacc caaactttcc gtttttcctt actgcagcca gtgactcgtg gaaagagcca
    13501 gcttcctcat actccggatt aattaccatt ttgcctgtac tgtcaagata gccccattta
    13561 ttttctgatt ttacagctgc aaaaccttcg ctaaaagcca tggcatcctg gtaaataaca
    13621 ggaacaatta attttccttg ctgatctaca tatccaaact ttccatcctt acttactttg
    13681 gccattccac aaacacaatc atccacaaaa tcatactgct tggtccaagg ctgggcctgc
    13741 actattgtta cggctaacaa tagccagggt aaaaagtatt ttttcatgct gagtggtttt
    13801 atgaatgaag aaattttctg gcgttaaggt agacctgtaa cctgccattc ccctatgacc
    13861 tgtggcagcc ctttgcatga ttaggagcat tcatccttct gacctcccta taccggtctc
    13921 cttccctcaa gaaattttaa aacggaacag aaattctctc tttttcgggc aaaaaatcat
    13981 tcaatcaccg taatcagcag taaatctgtg gtcattatct ttgccgctat gaatccatta
    14041 acagacaaga tgccatttac tgataaaaat aatttggcct ctttcaaccc cgacaaacag
    14101 cataccttga aatcttctgt cagcatttcc ggtacaggtt tgcataccgg aatcatggca
    14161 gatttgaccc tgaaacctgc cagcccgggc tttggttttc agtttcaacg aattgacctg
    14221 cccaaccagc ctataatcaa ggccgattgt gacctcgtta cagatgttag tcgtggtact
    14281 acactggaag ccaatggcgc caaagtgagc acagtggaac atgtattggc cgccctggta
    14341 ggcatgggaa tcgataactg cttattagaa attaatggcc cagaaatgcc cattatggac
    14401 ggtagttcgc aaccctttat cgaactaata gaagaagctg gtatagtgga acaagatgcc
    14461 gccaaagtgt ggtacagcat agacgagaac atctatcatt atgatgagtc gaaaagagta
    14521 gaaatggtgg ctatgccagc taccgaatac caggtaacga cgctgataga tttcaactca
    14581 ccggttttgg gcacccagca tgccgggtta aaacatatca gcgaattc
//
LOCUS       DAORLS010000039        14582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2214_length_14582_cov_2.626282, whole genome shotgun sequence.
ACCESSION   DAORLS010000039 DAORLS010000000
VERSION     DAORLS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14582
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2214_length_14582_cov_2.626282"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>364)
                     /locus_tag="PK977_04805"
     CDS             complement(<1..>364)
                     /locus_tag="PK977_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF17461.1"
                     /translation="VSMHYCPGCAHSLVHKLIMEVVEEMDIQEQTIGIAPVGCSVFAY
                     DYMDIDMQEAAHGRACAVATGIKKLMPDKYVFTYQGDGDLAAIGIAETLHAINRGENI
                     LIVFMNNAVYGMTSGQMAP"
     assembly_gap    365..411
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(474..1541)
                     /locus_tag="PK977_04810"
     CDS             complement(474..1541)
                     /locus_tag="PK977_04810"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRF17462.1"
                     /translation="MTETKLMKGNEAMAEAAILAGCDAYFGYPITPQSEVLEYLAREM
                     PKHNRIVLQAESEVASINMVYGAAGAGFRTMTSSSSPGISLMQEGISYIAGAELPCLI
                     VNVNRAGPGLGTIQPGQGDYFQATKGGGHGDYKMIVLAPASVQEMADFVYLGFDLADK
                     YRNPVMMLSDGAIGQMMEKVEFKTYTKPNIDKSWATTGKPKTRSRNYITSLFIQPERM
                     EQHNLKLEEKFKRIRENEVRFEEINTTDAEFLFVAYGLSARICQKAMDIAREKGIKAG
                     LFRPITLYPYPYQRLKELAKDVKLMLSVELNSGQMVEDVKLAVEGKVPVEYYGRMGGM
                     VPTPEDIVHHLETLIQYHHAN"
     gene            complement(1548..1877)
                     /locus_tag="PK977_04815"
     CDS             complement(1548..1877)
                     /locus_tag="PK977_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF17463.1"
                     /translation="MEDNTNKTATAKERKVKGRVEIDIQRCKGCELCTTACKEHALTL
                     SDTINIKGYRYIIANNDVCTGCVNCALVCPDAVITVYRTHPKKKPIDITPENIKEQLQ
                     TMISPSK"
     gene            complement(1990..2328)
                     /gene="hypA"
                     /locus_tag="PK977_04820"
     CDS             complement(1990..2328)
                     /gene="hypA"
                     /locus_tag="PK977_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRF17464.1"
                     /translation="MHELSIAQSILAIAQKAAPPDVIITGVNIQVGELSSIETESLKF
                     AFTAIRDETILQKADLNIDIIPGEAQCADCSNIFHLPAFGHPCPRCQGFSLKILKGRE
                     MKVTSLTVEE"
     gene            complement(2345..2836)
                     /locus_tag="PK977_04825"
     CDS             complement(2345..2836)
                     /locus_tag="PK977_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF17465.1"
                     /translation="MKTFFKTIGNVFKKPVTTKYPFEKTFIPEDYRGLIGFDENLCIW
                     CRRCEMVCPPGAIVFSQDMEGKQTYHFNRAVCIYCGECVRACPKEGAIFQTAEPAPCA
                     LKTENINNGWNKLYAEALQSREAYAAEKKKKAAEKAAADKLAAAKKEEENKSATDNQT
                     GAV"
     gene            complement(2847..3935)
                     /locus_tag="PK977_04830"
     CDS             complement(2847..3935)
                     /locus_tag="PK977_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRF17466.1"
                     /translation="MAKQYEIPIGAQHISLLEPLHFKFTTENEKIVKTDANVYYVHRG
                     IEQACCSKFKFRQVSFVVGRVCGLCSISHTTAYSQVAEKLVKIEVPKRAKYLRMLVIE
                     LDRIHSHLLCLSHVAENSGFEALFMKTMQYREFSMELLEAVCGNRIQYDFSIVGGVAR
                     DLKPQVAQSIIERLKKFVPQLDELMDIYKNNWTLSLKNKGAGAITKEDAMRLNVVGPF
                     ARAAGLAVDVRNETEDLLPWKEVGYEMITETSGDVYARNIVRLRELYVSIRIIENIIN
                     GLPETPLITETKAFPDGEATVRLEAPRGELFYYAKGNKTQVLERLKIKAPTFSNVAAM
                     AEAFTGNEYGSVPAIIASYDPCLSCTAR"
     gene            complement(3938..4279)
                     /locus_tag="PK977_04835"
     CDS             complement(3938..4279)
                     /locus_tag="PK977_04835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRF17467.1"
                     /translation="MKKIETTLTSLKSDIAAFYKQELHHFMVMNAVEVADKIEVQWFF
                     SDYAYLCETTCFFTLIDPAEKIPSIKDIVVSAWVAEAELVDLMNINIENTAKGFVLEP
                     DFESGPLRKKK"
     gene            complement(4276..4800)
                     /gene="nuoB"
                     /locus_tag="PK977_04840"
     CDS             complement(4276..4800)
                     /gene="nuoB"
                     /locus_tag="PK977_04840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRF17468.1"
                     /translation="MKLASYRKKSPWILHYNAGGCNGCDIEILACLSPKYDIERFGMI
                     NTGNPKQSDILLVTGPVTKRNRERLVEIYAQMPEPKVVIACGACASTGGVFRGMYNCE
                     GGIDQYIPVDVYVCGCGTRPEQIIDGVVQALAILETKTKELEESVRLKKEAVKEKQQL
                     NRRNIADEIKPAFT"
     gene            complement(4797..>4995)
                     /locus_tag="PK977_04845"
     CDS             complement(4797..>4995)
                     /locus_tag="PK977_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17469.1"
                     /translation="FCYALVYLFAGNNMLLGAGLIVFTFFYLNALDNSTARVNYKQML
                     RFSLAVSFSLAFINLLINSFS"
     assembly_gap    4996..5009
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5010..>5367)
                     /locus_tag="PK977_04850"
     CDS             complement(<5010..>5367)
                     /locus_tag="PK977_04850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRF17470.1"
                     /translation="HLLSTALLILAAYSTRSIFSHLGANRTAISILAYEPVLIIIAVS
                     IFILTGSFDAAAAFQSEQPLLYKMPLAFIALLLVLPIKLKKSPFDVAEAHQELTGGVE
                     LEFSGIFYEAVYTAKWL"
     assembly_gap    5368..5399
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5400..5649)
                     /locus_tag="PK977_04855"
     CDS             complement(<5400..5649)
                     /locus_tag="PK977_04855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRF17471.1"
                     /translation="MNNLWIPIILIILAPFIGGLVYGFERIIKARMQRRIGPPLLQPF
                     YDFLKLADKRKMIIHSAHAFLGIMHFVSLWFALGVLLLG"
     gene            complement(5660..7525)
                     /locus_tag="PK977_04860"
     CDS             complement(5660..7525)
                     /locus_tag="PK977_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF17472.1"
                     /translation="MYSLITSLFVIPVLISLLLLFVHRSLAKPLVITASLFLSAVSVY
                     LFINAEGVYYFGVPAYVNDIVAVADVMLLVFFGWIAIKRRSWIVGLMSLLQLGGLLYL
                     LNNMPVHHSLQFMVDKLSLFMFLLINIISGIIAVYSLRYIDEEHCSLFRKKYFLSTIF
                     WFIAVMNLVVSSDNLEYFFLFFELTTLASFLLIGFRKDEVSVKNSLTALWMNQVGGLA
                     ILAAIFFIHHNGYGEATFTNLLANVKAEGILLPLALLSMAALIKGAQMPFSKWLLGAM
                     VAPTPVSALLHSSTMVKIAPFIILRLSPALKDTPVAYVIIALTGFVFIAAAIGALSQD
                     NFKRILAHSTIALLALMIMMAAVAAPVTITASLILVLFHGISKSMLFLNAGILEKVFH
                     LKQSSDMDKLGESGPFTALVITIGFMSLLLPPFGAFIGKWMSIESLGALATDKKILGA
                     LAMVAVAGGGAVLSLLYFKVMGVLIARTGTDDKIQFEKTGPYYAVTTYLLLGLLLATV
                     ISLPLLLNQYFAPVAGSLSGTPLTITTEGWTMFLGSIKLPLIPMLIAFLLLPVSVIAA
                     MFVRFKNVDRAKEYMCGEKVSYSFSSFYFSTDRATPYFATAGILFFVALIIVALI"
     gene            complement(7636..8115)
                     /locus_tag="PK977_04865"
     CDS             complement(7636..8115)
                     /locus_tag="PK977_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17473.1"
                     /translation="MKNSKTEKGNILLIGIGNSGRRDDGLGWKFTDMARHLGYKEMDC
                     EFRYQLQVEDVLMVCRYEKVVFADASHTSIKEGFEIKPIKPAGHYFFSSHMQSPETIL
                     YLAKELYNKTPEAYTLAISGHNWGLGTVLSRAAQENLQKAFRFFKSRFVLSSKESAE"
     gene            complement(8096..9547)
                     /locus_tag="PK977_04870"
     CDS             complement(8096..9547)
                     /locus_tag="PK977_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRF17474.1"
                     /translation="MTRKITIDPVTRVEGHGRVTIHLDEYGQVKDSFFHIVEFRGFER
                     FIQGHPYWEAPVLVQRLCGICPVSHHLAAAKAIDQIVGVDPTDLSPTATKLRRLMHYG
                     QVFQSHALHFFYLASPDLLFGVDAPAEKRNVVAVAVENRELAVKGITMRKYGQEIIKA
                     VAGKKIHGILAVPGGVHKTFTEKERDYFLDGKNIENIDTMIEWSLAMVDFMKGYHEAN
                     RKFLDDFAYFPSGHLGMVNDFGGMDLYDGKLRAIDSEGKRTLDDISTNLYQDYFLEAA
                     SQWSYMKFPYLKQLGREKGWNRVGPLARINVCDFITTPLADKARKEFIAFTGKKVNNS
                     TMYSHWARLIEVLHCAEEMKLLLNDPELMGSDLVRKGTPRHKGIGIIEAPRGTLTHHY
                     EVDDKGMITKCNLIVSTTHNNDAMNTAVRWVANNVISRKGHITDGMLNQVEVAIRAYD
                     PCLSCATQAMGKMPLKAELYNDKGELIDEKFKN"
     gene            complement(9558..>10425)
                     /locus_tag="PK977_04875"
     CDS             complement(9558..>10425)
                     /locus_tag="PK977_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRF17475.1"
                     /translation="NPQRMVMEHNRCIKCLRCVVEVKTSDGKKVFNYQERGNETFVGI
                     DYEQEAKLTKEQALNAMHICPTGAIIVRGESLAAPFGDRKFDMESVQKDYNKKKTTHK
                     NRPPVMGKKIVATVSLAGCFGCHMSLLDIDEDLLDVVELISFDKSPLTDFKEFTSRCH
                     IGIIEGGVCNSENVETLRKFREHCDILVSLGECSVWGGLPSMRNSIPLSECLEEAYLN
                     SATSEPGANIIPYHEDLPKILDRVYACNEIVKIDYYIPGCPPDANHIWKAVKNILWGE
                     EYSILYSEFKYD"
     assembly_gap    10426..10480
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10481..10814)
                     /locus_tag="PK977_04880"
     CDS             complement(<10481..10814)
                     /locus_tag="PK977_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF17476.1"
                     /translation="MAEMVNITIDGKSIQAEKGKNLMQVAKENGIFIPSLCYYEHIEP
                     PLGTCRVCTCKMNGKYNPACTEKVSEGLVVEVNTPELTDTRKALVEMMFAEGNHICPA
                     CEKSGNCDL"
     gene            complement(10820..12583)
                     /locus_tag="PK977_04885"
     CDS             complement(10820..12583)
                     /locus_tag="PK977_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRF17477.1"
                     /translation="MPAETVQQLVEPGQTQMLNQLWELQLKNGFINDDDIKKIAEQFN
                     LSTIEVEGVVSFYHFFHRKPTGKYTIYLNNSIIADIKGFKRIKETFERETGAVFGSVD
                     RTGTFGLFLTPCIGLSDQEPSALINFYPFTNLNAIRVKDIINALNNGATPESICDAPA
                     DNIRYTPPGEKSIFFKKFEDGKALTHAEKLGADGVIEALKKTELAGRGGAYFPTWKKW
                     KAAKEQTTTPKFVVCNADEGEPGTFKDRVLISTQATSMIEGMMICGYTIGAEYGIIYL
                     RGEYRWLKPKLDEAIDDYRKKGLLGKDCGNIKGFHFDIRVQMGAGSYVCGEETALLES
                     LEGKRGEPRTKWFFPVEKGYLQLPTVINNVETFCAAARILEMGAEQYMQMGIPGSPGT
                     KLISVSGDCKFPGIYEIEWGMTVAELLELCEADDPYYIQVSGPSGECISIKEKYRRIS
                     MLDLLARKDIRCGGSFMIFNKQRDLVKVLLNFSEFFKSESCGICTPCRAGNFIIQRKL
                     ERLDNGLANEKDLEELKSWGTIMKNTSRCGLGKTATNSLIFSMEKFTDYFRGKLDKEY
                     NGLNLKFDMEEATEDYEKYKI"
     gene            12811..13011
                     /locus_tag="PK977_04890"
     CDS             12811..13011
                     /locus_tag="PK977_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17478.1"
                     /translation="MKKIAIVSLFTALFPFLGFAHEGHGHTHGFTITHYFVEPEHLLL
                     LIGVLAAGILFFRSYFKKQKKV"
     gene            13038..13805
                     /gene="hypB"
                     /locus_tag="PK977_04895"
     CDS             13038..13805
                     /gene="hypB"
                     /locus_tag="PK977_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRF17479.1"
                     /translation="MCATCGCDSNGNISFHEPGHEHHHNHDHGHSHSHEKTVVEVEQD
                     ILHQNNLLAQRNRGYFDAKNILAINMVSSPGSGKTALLERTLKDLKDQFDFYVIEGDQ
                     QTNLDADRIHATGTKVVQINTGKGCHLDAHMVLHALQGMKPKENGVMFIENVGNLVCP
                     AMFDLGEKERVVIMSVTEGEDKPLKYPEMFHTSTLCIINKTDLLPYVPFNIERAKAYA
                     RQVNHNLEIIEVSCLTGEGLPQWYEWLRAKVPVLQPA"
     gene            13808..>14582
                     /locus_tag="PK977_04900"
     CDS             13808..>14582
                     /locus_tag="PK977_04900"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRF17480.1"
                     /translation="MKTYHIHIGGLVQGVGFRPHVYKIAEQMALTGWVSNTKDGVHIE
                     INAEETVSALFYRSVIQQPPDNAVITAHSIKEVAPKTFSSFSILPDNTDSKPDLLLTP
                     DFAICEHCKNETADPKNRRYRYPFTTCLHCGPRYSIIKALPYERYHTTMSHLPMCEDC
                     SDEYNNVYDRRHYSQTNSCNKCSIPMHLFSAAGKIISHQPEEILINCKQYFAAGNIIA
                     VKGIGGYLLLCDATNESAVQQLRSRKHRPARPFALLYASI"
BASE COUNT         3842 a         3251 c         3089 g         4252 t
ORIGIN      
        1 aggcgccatt tgcccgctgg tcattccgta aactgcatta ttcatgaaaa cgatcaaaat
       61 attttcacca cggttaatgg catgcaatgt ttcggcaata cctatggcag ccagatcacc
      121 atcaccctga taagtgaaaa catatttatc cggcatcagt tttttaatac ctgttgccac
      181 cgcacaggca cggccgtgtg ctgcctcctg catgtcaata tccatataat catacgcaaa
      241 gacagaacag cctacaggtg caatgccaat ggtttgttcc tgtatatcca tttcttccac
      301 cacttccatg atgagtttgt gtacgaggct atgggcacag cccggacagt agtgcatact
      361 cacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttcctcat
      421 cgtggcaggc cggtgttacc acatccagcg ctgtatcggg taatacatgt tcttcagttg
      481 gcatgatgat actgtattaa agtttctaaa tgatgtacaa tgtcttccgg agtgggtacc
      541 attccgccca ttcttccgta gtactcaacc ggtacttttc cttccacagc cagtttcaca
      601 tcttctacca tttgcccgct gttgagttcg acactcagca tgagttttac atctttagca
      661 agttctttca gtcgctggta agggtagggg tataatgtga tggggcggaa caatcctgct
      721 ttgatgcctt tttctcttgc aatgtccatt gctttctggc agatacgggc actgagtccg
      781 taggctacaa aaagaaattc ggcatcggtt gtatttattt cttcgaacct tacttcattt
      841 tcccggatgc gtttgaactt ttcttccagc ttcaggttat gttgttccat tctttccggc
      901 tgaatgaaga gagaagtgat atagttcctg ctacgggttt ttggtttacc ggtagtggcc
      961 catgatttat caatattcgg tttggtataa gttttaaact ctactttttc catcatctgg
     1021 ccaatagcac catcagagag catcatcacc gggttacggt atttatcagc cagatcaaat
     1081 ccgaggtaaa caaaatcagc catttcctgt acagaggcag gcgccagtac gatcatttta
     1141 taatctccat ggcctccgcc ctttgttgcc tggaaataat caccctgccc gggttgtatg
     1201 gttccgagcc ccgggcctgc tctattgaca tttacaatta aacatggcaa ctctgcaccg
     1261 gcgatatacg aaattccttc ctgcatcagg ctgatacccg gagaagagga actggtcatg
     1321 gtccgaaagc ctgcaccggc agcgccataa accatattga tagaagctac ttcactttct
     1381 gcctgcaaca caatgcgatt gtgtttgggc atttctcttg ccaggtattc cagtacttct
     1441 gattgcgggg tgatgggata gccgaagtag gcatcgcagc cggccagaat ggcggcttct
     1501 gccatggctt cgttaccctt catgagtttg gtttccgtca taacaaatta ttttgaagga
     1561 ctgatcattg tttgcagttg ctctttgata ttctccggtg tgatatcaat cggttttttc
     1621 tttgggtggg ttctgtatac tgtgatgact gcatcgggac aaaccaatgc acagttgaca
     1681 cagccggtgc atacatcatt attggcaatg atgtaacgat agcctttgat gttgatggta
     1741 tcagaaagag tcagtgcatg ttctttgcag gcggtagtgc agagttcgca gcctttgcat
     1801 ctttgtatgt caatttcaac ccttcctttg acttttcttt ccttcgccgt agcggttttg
     1861 ttggtgttgt cttccattat ccgggtttta gagctataac aaatgtccaa atatgccggc
     1921 ccgattcaca gttgccccgt catggctggg tatgatttat gtcagttttt cattggaaaa
     1981 acagaggatt tactcctcta cggtgagact tgtcactttc atctcccggc ccttcaggat
     2041 ctttaaggaa aagccctggc agcgtgggca gggatgtcca aaagcaggca agtgaaaaat
     2101 gttgctacag tcagcacatt gtgcctcccc gggaatgatg tcgatgttca gatcggcttt
     2161 ttgtaaaatt gtttcgtctc tgatagcggt aaaagcaaat tttaacgatt cagtttcaat
     2221 actgcttaat tctcctacct gaatgtttac accggtaata atgacatcag gcggggctgc
     2281 tttttgggca atggcaagaa tagactgtgc tatagagagc tcatgcatat tgaataagat
     2341 agatttatac agcaccggtt tgattatcgg tggctgattt attctcttcc tctttttttg
     2401 ctgcagccag cttatcagct gccgcttttt ctgccgcttt ctttttcttt tcggcagcgt
     2461 aagcttcacg gctttgcagt gcttctgcat ataatttatt ccagccgtta ttaatgttct
     2521 ctgttttcaa tgcacatggg gctggttctg ctgtttggaa aatagcacct tctttggggc
     2581 aggcacggac acattcgcca caatagatac aaacagccct gttgaaatga taggtttgtt
     2641 tgccttccat atcctgtgaa aaaacaatag caccaggcgg gcaaaccatt tcacaacgac
     2701 ggcaccagat gcagaggttt tcatcgaagc cgatcagtcc ccggtaatcc tcgggtataa
     2761 atgttttttc aaacgggtat ttggtggtga ccggtttctt aaatacgtta cctattgttt
     2821 taaaaaatgt tttcatcatt caagttttat ctggctgtac aggaaaggca agggtcataa
     2881 gaagcaatga tagccgggac tgaaccatat tcattgccgg taaatgcttc tgccatagct
     2941 gctacattgg agaatgttgg cgccttgatc tttagtcttt ccagtacttg tgttttgttc
     3001 cctttggcat aatagaacaa ctctcctctt ggagcctcaa gccttacggt tgcttcgccg
     3061 tcaggaaaag cttttgtttc ggtgatcaat ggtgtttcgg gcaagccgtt aatgatattt
     3121 tcaataatgc gtattgatac atacaactcc ctcaggcgaa caatgtttct tgcatacaca
     3181 tcgccactgg tttctgtgat catttcataa cctacttctt tccagggaag caggtcttct
     3241 gtttcgttgc gaacatctac tgctaatcct gcagctctcg caaaaggtcc caccacatta
     3301 agccgcatag catcttcttt ggtgatggct cctgcgcctt tgttcttcaa ggacagcgtc
     3361 cagttatttt tgtagatgtc cataagttca tctagttgcg gaacaaattt tttcagcctt
     3421 tcaataatcg attgcgcaac ctgtggtttc agatccctgg ccacaccacc cacaatggag
     3481 aagtcatact ggatgcggtt gccgcaaacg gcttcgagta gctccataga gaactcccgg
     3541 tactgcatgg ttttcatgaa cagtgcttcg aagccggaat tttccgccac atggcttaag
     3601 caaagtaagt gtgagtgaat gcggtccagt tcaattacca gcatccgtaa atattttgct
     3661 cttttaggca cttctatctt caccagtttt tctgcaacct gtgagtaggc agtagtgtgt
     3721 gaaatggaac agagtccgca aacccttccc acaacgaaac ttacctggcg gaatttgaat
     3781 ttggagcagc aggcctgctc tatgcccctg tgtacataat agacattggc atcagtcttc
     3841 actatttttt cattctcagt agtgaattta aaatgcaggg gttccagcag ggaaatatgc
     3901 tgggcgccta ttggtatttc atattgtttt gccatgatta tttcttttta cgcagcgggc
     3961 cgctttcaaa atcaggttca agcacaaatc cttttgctgt gttttcgata ttaatattca
     4021 tcaggtccac cagttcagct tctgccaccc aggcggatac tacaatgtct ttaatgctcg
     4081 gaattttctc tgccggatca atcagtgtaa agaagcaggt agtttcgcag aggtaagcat
     4141 aatcactaaa aaaccactgc acttctattt tgtcagccac ttccactgca ttcattacca
     4201 tgaaatgatg gagttcctgt ttgtaaaaag cagctatgtc gctttttaag gaggttaatg
     4261 tggtttcgat ctttttcatg tgaatgccgg ttttatttca tcagcaatat ttcttctgtt
     4321 tagttgttgt ttttctttta ccgcttcttt ctttagccgc actgattctt caagctcttt
     4381 tgtttttgtt tccagtatgg ccagggcctg caccaccccg tcaataattt gctccggcct
     4441 tgtgccgcat ccgcatacat acacatctac aggaatatac tggtcgatac caccctcgca
     4501 attatacatg ccccgaaaca caccgcctgt actggcacaa gcgccgcagg ctattaccac
     4561 tttgggttcg ggcatttgtg catagatctc caccagccgt tcacggttgc gtttggttac
     4621 cggtccagtc actagtaaga tatctgattg tttgggattg ccggtattga tcatgccgaa
     4681 acgttcaata tcatatttag gactgaggca ggccagtatc tcaatatcac atccgttgca
     4741 gccgcctgcg ttgtagtgca gtatccaggg agatttcttt ctgtatgaag ccagtttcat
     4801 gaaaaactat ttattagtaa gttgataaag gcaagggaaa atgaaacggc taatgaaaac
     4861 cgtagcattt gcttatagtt aacccttgca gtggaattat cgagtgcatt caggtaaaag
     4921 aaagtgaata ctatcaatcc ggcgcccaat aacatattat tacctgcaaa aagatagacc
     4981 aatgcatagc agaatnnnnn nnnnnnnnnc cagccatttg gcggtgtaca ccgcttcata
     5041 aaagataccg gaaaattcaa gttctacgcc gcctgttagt tcctggtgag cttcagccac
     5101 atcaaagggt gattttttta acttgatcgg tagtaccagc agcaaagcaa taaaggccaa
     5161 tggcattttg tacagcaaag gctgttctga ttgaaaagct gctgctgcat caaagcttcc
     5221 ggttagtatg aagatagaaa cagcaataat aataagtacc ggttcatagg ccagtatact
     5281 gatagcggta cgatttgcac cgaggtgact gaaaatagaa cgggtgctgt aagctgccag
     5341 tattaataac gcagtggaaa gcaggtgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5401 ccccaatagt aaaacgccta aagcaaacca cagtgataca aagtgcatga taccgagaaa
     5461 agcatgtgct gaatgaatga tcatttttcg tttgtctgcc agcttcagga aatcataaaa
     5521 gggttgcagt agcggtggcc cgatacgccg ttgcattctt gctttgatga tgcgttcaaa
     5581 accatatacc agtccgccaa tgaagggagc cagtataatc agtattatcg gaatccagag
     5641 gttgttcata ataatcagtt taaatcaatg ccacgatgat caatgctaca aaaaacagga
     5701 tacctgctgt agcgaaataa ggagtggccc tgtcggtaga gaaatagaag gaggaaaaag
     5761 aataactcac tttttccccg cacatatatt ctttggccct gtctacattt ttaaacctga
     5821 caaacatggc tgcgataaca ctgaccggta ataacagaaa agcgatcagc ataggtatca
     5881 gcggcagttt gatagagccg agaaacatag tccaaccttc tgttgtaatt gttaatggtg
     5941 tacctgataa agatcctgct accggtgcga aatactgatt cagcaataag ggcaggctta
     6001 tgacagtcgc cagcagcaat cccagcaaga gataagtagt gacagcatag taagggccgg
     6061 ttttttcaaa ttgaattttg tcatcagtgc cggtacgggc aatcagcaca cccattactt
     6121 tgaaatataa aagtgatagc accgcacctc cgccggctac agcaaccata gccagtgcac
     6181 ccagtatttt tttatcagta gctaatgctc cgagtgattc aatgctcatc catttaccaa
     6241 taaaagcgcc gaatggcggc agcaataaag acataaaacc aatggtaatg actaaagctg
     6301 taaaaggtcc gctttccccc agcttatcca tatcggatga ttgcttgagg tgaaatactt
     6361 tttccagtat gcctgcatta aggaagagca tggatttgga gataccatga aacagcacca
     6421 gtatcaatga agcagtaata gtgacaggtg cagctactgc cgccatcatg atcattaatg
     6481 ccaataaagc aatggtggaa tgtgccagta tccgcttgaa attatcctgt gacaaagctc
     6541 caatagcagc agcgatgaac acgaagcctg tcagcgcaat gatgacgtaa gccaccggtg
     6601 tatcttttaa agccggtgat aaacgaagga taatgaacgg ggcaatcttc accatggtgg
     6661 aggaatgcag cagggcactg acaggagttg gcgccaccat cgctccgagc agccatttgc
     6721 tgaaaggcat ttgtgcccct ttgataaggg ctgccatact caataaagcc agcggaagta
     6781 aaatgccctc agcttttaca tttgccagca ggttagtaaa tgtagcctca ccataaccgt
     6841 tgtggtggat aaagaaaata gctgccaaaa ttgccaggcc accaacctgg ttcatccata
     6901 aagccgtcag tgaatttttt acagatactt cgtccttcct gaaaccaatc agtaaaaaag
     6961 aggccagtgt agtcagttcg aagaacaaaa agaaatactc cagattatcg gatgaaacaa
     7021 ccaggttcat tacagcaatg aaccagaaga tggtggagag aaaatatttt tttcgaaaaa
     7081 ggctgcaatg ttcttcatca atatacctga gtgaatacac agcaatgatg ccgctgatga
     7141 tattgataag gagaaacatg aacaggctca gcttgtctac catgaattgc aatgagtgat
     7201 gcaccggcat attattcaac aggtataata aaccgcccag ctgcaacagg ctcatcagtc
     7261 caactatcca gcttcttctt ttaatagcga tccagccgaa gaaaacaagc agcatgacat
     7321 cggccactgc cacaatatca ttcacataag ccggaacgcc gaaataataa acaccttcgg
     7381 cattgataaa taaataaact gatactgcag agaggaataa ggatgctgtt atgaccaatg
     7441 gttttgccag cgacctgtga acaaacaata gcaggagact gatcagaacg ggtattacaa
     7501 acagcgaagt aattagtgag tacatgaccc ggattttagc tgtaaatgag gatgctatat
     7561 taagccgaat tggccgggtg gggtatgatg cgtatcagat gtaaagatga tatggcttat
     7621 gctgctaact aatccttatt ctgctgattc tttgctggac agaacaaatc tggatttaaa
     7681 aaatcgaaag gctttttgca ggttttcctg tgccgccctg ctgagtactg tacccagccc
     7741 ccaattatgc cccgatatgg caagtgtata tgcctccggt gttttattgt acagttcttt
     7801 ggccaggtag aggattgttt ccgggctttg catgtgagag gagaagaaat agtgcccggc
     7861 aggttttatt ggttttattt caaatccctc ttttattgaa gtatgtgatg catctgcaaa
     7921 caccactttt tcgtagcggc aaaccatcaa tacatcttcc acttgcagct gataacggaa
     7981 ttcacaatcc atctccttgt atcctagatg cctggccata tcggtaaatt tccagcccag
     8041 cccgtcatcc cggcggccgc tgttgcctat accaatcagt aatatattac ccttttcagt
     8101 ttttgaattt ttcatcaatt aattctcctt tatcattata caattctgct ttcaacggca
     8161 tttttcccat cgcctgtgtg gcacaactga ggcagggatc ataagccctg atggctactt
     8221 ctacctggtt cagcataccg tcagtgatat ggcctttgcg gctgatgacg ttattggcca
     8281 cccatctcac ggctgtattc atcgcatcgt tgttatgggt ggtggaaacg atcaggttgc
     8341 atttggtgat catacccttg tcatctactt cgtagtgatg ggtcagggtg cctcggggag
     8401 cttcgataat gccaatacct ttatgccggg gtgttccttt acggacaaga tcgctgccca
     8461 ttaattcagg atcattcagc aatagtttca tttcttcagc acaatgcagc acttcaatca
     8521 gccttgccca gtgtgaatac atcgtactgt tgttgacttt tttaccggtg aatgcaataa
     8581 actctttccg ggctttatct gccaacggag tagtgataaa atcgcaaaca ttgatccttg
     8641 ccagcgggcc tacccggttc catccttttt ctctgcctaa ttgttttagg tagggaaatt
     8701 tcatatacga ccattgtgat gcagcttcta aaaagtaatc ttgatagagg ttagtggaaa
     8761 tatcgtccaa tgttcttttt ccttcgctgt caatggctct cagtttgccg tcgtacagat
     8821 ccattcctcc aaaatcattc accataccga ggtgaccgga agggaagtaa gcaaaatcat
     8881 cgaggaattt tcggttggct tcatgatacc ctttcataaa atccaccatg gccagagacc
     8941 attcaatcat ggtatcgata ttctcaatat tctttccgtc aagaaaataa tctctttctt
     9001 tttcagtaaa tgttttatga acaccaccgg gcactgccag tatgccatgt atttttttac
     9061 cggccacggc tttaatgatc tcctgcccgt atttgcgcat ggtaatgcct ttcaccgcca
     9121 attcacggtt ttctactgca accgccacca catttctttt ttctgccgga gcatctacac
     9181 caaacagaag atccggtgaa gcgagataaa agaagtgtaa tgcatgcgac tgaaagacct
     9241 gtccatagtg cattaacctg cgaagtttgg tggcagtagg tgaaagatcg gttggatcaa
     9301 caccaacgat ctggtcaatt gctttggcag ctgccaggtg atggcttacg ggacaaatac
     9361 cgcaaagccg ttgtaccagc acaggtgctt cccaataggg atgaccttgt ataaaacgtt
     9421 caaagcccct gaactctaca atgtgaaaga aggaatcttt cacctggcca tattcatcga
     9481 gatgtatagt aaccctgcca tggccttcca ctcttgttac aggatctatg gttatttttc
     9541 gtgtcatgtt acattcatta atcgtacttg aattctgaat acaaaatgga gtactcttct
     9601 ccccacaaaa tattcttcac tgctttccag atatgattgg catcaggagg gcagccgggg
     9661 atataataat caatcttcac tatttcattg caggcataca ccctatccag gatcttgggt
     9721 aaatcttcgt ggtagggaat gatgttagcg ccgggctcac tggtagcaga gttgagatag
     9781 gcttcctcca aacattcact taaaggaatc gaattgcgca tggaaggcaa gccgccccat
     9841 accgagcatt ctcctaatga aacaaggata tcacaatgct ccctgaattt tcgcagtgtt
     9901 tccacatttt ctgaattaca cacaccacct tctataattc caatgtggca acgggatgtg
     9961 aattctttaa aatctgtcaa tggtgatttg tcaaatgaga tcagttctac cacatccagc
    10021 aggtcttcgt caatatccag caacgacata tggcagccaa agcaacctgc cagcgaaaca
    10081 gtggccacaa tttttttacc cattaccggt ggccggtttt tgtgtgtcgt ttttttcttg
    10141 ttgtaatctt tctgcactga ttccatatca aattttctat cgccaaatgg agcagctaaa
    10201 ctttctcctc ttacaattat ggcgcctgtt ggacaaatgt gcatagcgtt cagcgcctgt
    10261 tcttttgtaa gttttgcttc ctgttcgtaa tcaataccaa caaacgtttc attacctctt
    10321 tcctggtagt tgaatacttt tttgccatca ctggttttta cttctactac acagcgcagg
    10381 catttgatgc aacggttatg ctccatcacc atccgctgcg gattgnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaaatcgca gttgccgctt
    10501 ttctcgcagg ccgggcagat atgattgcct tcggcaaaca tcatttcaac caatgcctta
    10561 cgggtatctg tcagttcagg tgtatttact tccaccacga ggccttcgga tactttttcc
    10621 gtacaggcag ggttgtactt gccgttcatt ttgcaggtgc acacccggca ggtgccgagt
    10681 ggcggttcta tgtgttcgta gtaacagagt gatggtatga atatgccatt ttcctttgcc
    10741 acctgcatca ggtttttacc tttttcggcc tgtatgcttt tgccatcaat tgttatgttt
    10801 accatttctg ccatgacctt cagattttat atttctcata gtcttccgtt gcttcttcca
    10861 tgtcaaactt cagattcagc ccattatatt ctttgtccaa tttgccgcgg aagtagtcag
    10921 taaatttttc catggagaat atcaggctgt tggtagccgt tttacctaag ccgcaacggc
    10981 tggtattctt cataatagtg ccccagcttt tcagctcctc caggtccttt tcattagcga
    11041 gaccgttgtc gagcctttct aattttcgct gaataataaa attacctgcc cggcagggcg
    11101 tacaaatacc acaggattct gatttaaaga attcagaaaa attcaacaac acttttacaa
    11161 ggtctctttg tttgttgaag atcatgaacg agccaccgca acgaatgtct tttctggcca
    11221 gcaggtcgag catggaaatg cggcgatatt tttccttaat agaaatacac tctcccgacg
    11281 gcccgctgac ctgtatgtag tagggatcat ccgcttcaca gagctccagc agttccgcta
    11341 ctgtcattcc ccattcaatt tcatatatgc cggggaattt gcaatcgccg gatacactga
    11401 tgagtttggt gccgggtgaa ccgggtattc ccatctgcat gtattgttct gcacccattt
    11461 cgagtatacg ggcagccgca cagaaggttt cgacattatt aatgacggtg ggcagttgca
    11521 ggtaaccttt ttcaaccggg aaaaaccatt tggttctggg ttcgcctctt ttaccttcca
    11581 atgattctaa taaagctgtt tcttcaccgc atacataaga gccggcgccc atctgaaccc
    11641 tgatatcaaa atggaagcct ttgatgtttc cgcagtcttt tcccagcaat cctttttttc
    11701 ggtaatcatc aatggcttca tccagtttgg gtttcagcca gcggtattcg ccacgcaaat
    11761 agatgatacc atattcagcg ccgatagtgt aaccgcaaat catcattccc tcgatcattg
    11821 atgttgcctg tgtactaatg agtaccctgt ctttgaaagt accgggttca ccctcatcgg
    11881 cattacaaac cacaaacttt ggagttgtag tttgctcttt tgcagctttc catttcttcc
    11941 aggtgggaaa gtaagcgccg cctctgccgg ccagttcggt ttttttcaat gcttcgatta
    12001 caccgtctgc tcccagcttt tctgcatggg tcaatgcttt cccatcttcg aactttttaa
    12061 agaaaatact tttctcaccc ggaggtgtat aacgtatatt atcagccgga gcatcacaga
    12121 tagattcagg agtggcacca ttgttcaaag cattaataat atccttgacc cgaatggcat
    12181 tcaggttagt gaaaggataa aagttgatca aggcagacgg ctcctggtcg ctgagcccga
    12241 tacatggtgt aaggaacaaa ccgaatgtac cggtgcggtc cacagagcca aagacagcgc
    12301 ctgtttcccg ttcaaaggtt tcttttatcc ttttgaaccc ttttatatca gctattatgc
    12361 tgttgttgag ataaatggtg tacttaccgg tgggcttccg gtgaaaaaaa tggtagaagg
    12421 aaacaacgcc ttccacttca atagtggata gattgaactg ttcggctatc ttctttatat
    12481 cgtcatcatt gataaaacca ttttttaatt gcaattccca taactggttg agcatttgcg
    12541 tttgcccggg ttccaccagt tgttgtactg tttcggcagg catagtcggg atttatggtt
    12601 tcagaaaacg actcaatatc aacacatttc actacagaag tgtatgacta ttgttaatct
    12661 gttatgtgat tttaagcaga ccagctttat ggcctaggca ggttattaaa aacagccttt
    12721 tacctgatta ttatcatatt cggctatgac agggttgttc tgtcctgcag cgcatatttt
    12781 ctaatattgg tgtctgtaaa acaaaccaaa atgaaaaaga ttgcaatcgt tagtttgttc
    12841 actgccttat ttcctttttt aggttttgca catgagggtc atggccatac acatggattt
    12901 accattaccc attattttgt agagcccgag catctgttat tgctgatcgg tgtattggca
    12961 gcagggattc ttttttttcg tagttatttt aaaaaacaaa agaaggtata atttattcat
    13021 gaacaaaaac gttagttatg tgtgctacct gcggctgcga cagcaatgga aatatctcct
    13081 ttcatgagcc cggccatgaa catcatcata accacgatca tggccattca catagccatg
    13141 aaaaaactgt agtggaagtg gagcaggata ttctgcacca gaataatttg ctggcgcaaa
    13201 gaaacagggg ttattttgat gcaaaaaata ttcttgccat aaacatggtg agctcacctg
    13261 ggtccggtaa aactgctttg ctggagagaa cattaaaaga cctgaaagac cagtttgact
    13321 tttatgtaat agaaggtgat cagcaaacta acctggatgc tgaccggata catgccaccg
    13381 gtacaaaagt agttcagatc aataccggta agggttgtca ccttgatgca catatggtgc
    13441 tgcatgcatt gcagggaatg aagccgaaag aaaatggtgt aatgtttatt gagaatgtag
    13501 gtaacctggt atgcccggcc atgtttgatc taggagaaaa ggaaagagtg gtgatcatga
    13561 gtgtaacaga aggtgaagac aaaccactga aatacccgga aatgtttcat acttctacac
    13621 tttgtatcat taataaaaca gatctgctac cttatgtgcc ttttaatatt gaaagggcga
    13681 aggcttatgc caggcaggtt aaccataatt tagaaatcat tgaagtaagt tgtctgaccg
    13741 gggagggttt gccccaatgg tacgaatggc tgcgggccaa agtgcctgta ttgcagcctg
    13801 cctgattatg aaaacctatc acatccatat cggcggcctg gtgcagggag tgggattccg
    13861 gccacatgta tataaaatag cagagcagat ggcgctgaca gggtgggtga gtaatactaa
    13921 agacggtgtt catattgaaa tcaatgcaga ggaaacagta tcagcattat tttatcggtc
    13981 tgtaattcag caaccgccag acaatgctgt tataaccgct cacagtatca aggaagttgc
    14041 accgaaaaca ttttcttcct tttcaattct tcccgataat actgatagca agcccgattt
    14101 actactcaca ccagactttg ccatttgtga gcattgcaag aatgaaacag cagatccgaa
    14161 aaacaggcga taccgttacc cgtttactac ctgtctgcat tgcggaccga ggtattctat
    14221 catcaaagca ttgccttatg agcgatatca tacaacgatg tctcatttgc caatgtgcga
    14281 ggactgtagt gatgaatata ataatgtata tgatcgccgg cattacagtc aaaccaattc
    14341 ctgcaataaa tgcagtatac cgatgcatct tttttcggct gccggtaaaa ttataagtca
    14401 tcaacccgaa gaaattctga tcaattgcaa gcaatatttt gctgcgggga atatcatcgc
    14461 agtaaaaggc ataggcggct acctgctgct atgtgatgcc accaacgagt cagctgttca
    14521 gcagttgcgc agcagaaagc accggccagc caggccattt gctcttttgt atgccagcat
    14581 cg
//
LOCUS       DAORLS010000040        14572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2218_length_14572_cov_1.882827, whole genome shotgun sequence.
ACCESSION   DAORLS010000040 DAORLS010000000
VERSION     DAORLS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14572
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_14572_cov_1.882827"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PK977_04905"
     CDS             <1..1580
                     /locus_tag="PK977_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF17481.1"
                     /translation="IKDKPFCTGWNSGLTILGMQDGTRTNAIAAPGKNATVWGTNAMD
                     SCWRFIPNGGVPRYKSAQLLVNGTVVATADTSTSAPGELNLNFNNVCPTVDSTAYVLR
                     VAYTNCNGPDDVIFTDTVFVKKLSPSITNLAKTDPTCTPDGTISVTGAGGTPPLSYSI
                     NGGTTYQASNVFTGLSGGTYTVIIRDGLGCISAIQTITLAPVNSLTQTVTKTDANCTT
                     SGSITINASGGGTPPYQYSINGGSTYQSSNVFTGLAAGTYTVITRNPVTQCTATQQVT
                     ITFTNNLTMNNIAGGTICRGASFTPQVSSNAASYSWSPSAGVSNTTIANPVLSPQNTT
                     TYTVTGTLGSCTIQRTVTVTVFEGATANAGPDAIILTGDVYQMLSSGTAGSYLWTPST
                     GLSSATVLSPTAAPQTTTTYTLTVTSPQGCTATDQMTLTVVPYCIKPMNAFTPNGDGI
                     NDRWLITDGNCLIGVRAQVFNRYGSRVFESPNYDNTWDGTYKGKPLPDGTYYYVLEFV
                     LLNGKRQVLKGDVTILR"
     assembly_gap    1695..1832
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1833..2508
                     /locus_tag="PK977_04910"
     CDS             <1833..2508
                     /locus_tag="PK977_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF17482.1"
                     /translation="TNAKIGIGGYLYNDVTGPTRRTGGQMAYSYHIQMANDATFSLGL
                     EARLQQFSLDKAKLSATLGNDPVLASSESRFKADAGFGIAYNGKKLQVGASVSQLIQS
                     KLDFYSGNLNPTEEGRLYRHYYLHGSYKWDVDGSTRIIPNLLFIYLPNAPLEVQGGAR
                     VEHNDVFWWGLGLRARQSWMISAGVKIQKKFTIGYCFDIFNTPLSVYDRGANGHEILL
                     RYDFLK"
     gene            2618..4855
                     /locus_tag="PK977_04915"
     CDS             2618..4855
                     /locus_tag="PK977_04915"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRF17483.1"
                     /translation="MDTVAQTPKYHLNEEEEKKLILREYRSLLKVIKNKLKPGDKNLI
                     RTAFEMAAEAHKTMRRKSGEPYILHPIAVAKICVEEIGLGVRSTICALLHDTVEDTDI
                     SLDDVRREFGEEITKIVDGLTKIANVIDVNASQQAENFKKILLTLTDDPRVILIKLAD
                     RLHNMRTLDSMKREKQLKISSETVYIYAPLAHRMGLYTIKTEMEDLAMKYMEPDVYKD
                     IARKLAETKKERTKYINEFIKPIKDKIVKANLEGDIYGRPKSIHSIWNKMKKKGVEFE
                     EVYDLFAIRVILDSPPEKEKEDCWKVYSMITDEYNPSPERLRDWLSNPKANGYEALHT
                     TVMGPQGKWVEVQIRTRRMNEIAEKGLAAHWKYKEGTSDESRFDKWFHQIREVIHNQE
                     TDSIDFLQDFKTSFLTEEIYVYTPKGDVKMLPVGSTALDFAFAIHSAIGVKTIGAKVN
                     HKLVPISHKLHSGDQVEIITSNKQKASEDWLGFVVTSKAKGRIRDSLKEEKRKVSDEG
                     RYTVQRKLEGLGAAMSQHNIDELVHWYKLPSNLDLFYQVAIKNIDLKELKDFKVIGDR
                     IEAPKPVKHVTEKHEFIPHIGKKDAELIIFGESSDKIVYNLAKCCNPIPGDDVFGFVT
                     TGKGLTIHRSNCPNAAKLLASYGHRVVKTKWAKNKEISFLTGIKIVGMDDVGVVSKIT
                     NLISGDLKINISALTIEAKEGLFEGNIKLYVHDKEELDNLVQKLLALPGIESVERYDM
                     EDIPS"
     gene            4958..>6288
                     /locus_tag="PK977_04920"
     CDS             4958..>6288
                     /locus_tag="PK977_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17484.1"
                     /translation="MRSIRLRIFSHIILGVITLFSTVTHYGQSCLPTNINGTNINLLC
                     NQICTTLVFRVPHLKSSDDYAVNTITYAPFAYTTPTGNELPTLYADDEYGPLVNLPFS
                     FCFYGANYNSVVVGSNSIITFDASTANQANAYPITQPIPYAGGTPNVISPAYYPPASI
                     MAGYSDIDPRASQSPPDRKIEWRIEGTAPCRRFIASYWHVGTFGGGVWSATGTNCNSQ
                     NPNNIQIVIYESTGIIDIFIGNKICIPSTNGGNSILGIQNWARNAARWAPGKNAVQWT
                     STNEGYRFTPAGGTSKFISSQLFTLTGTLVATADTSTTTAGLLDISFPNICAPAGPTQ
                     YEVVTTFSACDNPANLLVSRDTITLNRTNTLGATSSFTNTTCGPPSGTITVTIPAGVG
                     TAPFSYVLDGGTPVVTPALTYTFTGVAAGPHTVVVTDASTGCSSTINVNIT"
     assembly_gap    6289..6374
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6375..>8620
                     /locus_tag="PK977_04925"
     CDS             <6375..>8620
                     /locus_tag="PK977_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17485.1"
                     /translation="TNGSAPYSYVLDGGVPVSGGSPYTFTGLSAGNHTIVITDASGCI
                     SNTITVNVATGSGVNVSTTSTATSCPGASNGTITVNASGGVPAYTYQLGANPPQASNV
                     FTGLASGTYTITVRDNVGCIRTVSRTVTAGSAIVASMNTTGTTCSGAANGSVTVTPTN
                     GTGPYTFVLDGTVTQTGATNTTFTNLSAGPHTIIITDNPSGCLSNTININIPAGPALA
                     ASVTSSATTCSGASNGTITVTPTNGTGPYEFSLDGGPLVSGAVPYIFTNVPAGSHTIQ
                     VTDIPTGCISTIYTTLVSAGPVLLTTASKTDVLCNGGATGTITVAQPAAGTAPYEYSL
                     DNVNWQSSPVFTGLTAGTYTVYYRESNGCSNSLSITVNEPSTLTATDASTAVICNGET
                     NGTITVTANGGVSPYDYSIDGGANWQTGNIFTVAAGAYTVIIRDANGCTVSRNITVTA
                     VLTANSANTNASCDGGNDGLIDVAAVGGNGNYQYSINGTTFQSSNIFNVAPGTYTVTV
                     RDNLGCTTSFSTTVGLSSNITFTPQVDPTICEGSSAQLQLTSNATQYSWSPAVALSDT
                     TIYNPVANPVVTTQYIVTATLGRCSIIDTVIVNVNPAPVPDAGPAGYICYGQTYQLQA
                     SGGVQYQWTPATYLNNPNIANPVSSAPQDITYTVSIIADANGCPSLTTDDITIDVTPP
                     IKINTFPYDTIGYPGDQIQLLAIPTDTDATTIVWTPSTGLDNPFVPNPIVTIGQVGDD
                     RVYQVTAST"
     assembly_gap    8621..8639
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8640..8939
                     /locus_tag="PK977_04930"
     CDS             <8640..8939
                     /locus_tag="PK977_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF17486.1"
                     /translation="VRVRVYKGPDIYVPSGFTPNGDGRNDRFTPFPVGIKSLTYFRIF
                     NRWGQQIFSTNRLHDGWDGTLNGTAQPSGVYVWMIQAVTDDNRVINKKGTVTIIR"
     assembly_gap    9094..9110
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9111..10365
                     /locus_tag="PK977_04935"
     CDS             <9111..10365
                     /locus_tag="PK977_04935"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRF17487.1"
                     /translation="LLGLQWGDEGKGKIVDFFAKEYDLVARFQGGPNAGHTLYVNDKK
                     VVLHQIPSGVFHEGIINLIGNGVVLDPVTLKRECDTVASFGIDLRKNLFIAERTHLIL
                     PTHRALDKAAELSKGQQKIGSTLKGIGPAYMDKTGRNGLRVGDLLDKNFTTSYIKLRL
                     KHQRLLDNYNFQEDISQWEEEFFEAVEFLKQLNIVNGEYFINSNLKAGKKILAEGAQG
                     SMLDIDFGTFPFVTSSNTISAGVCSGLGIAPGHIRDVIGVTKAYCTRVGGGPFPTELE
                     NETGEELRKIGNEFGATTGRPRRCGWIDLVALNFACMINGVTKLVMTKADVLDAFKEL
                     QLCTDYKINGKQTREIPFQMERQKPEPVYKQFAGWNTATDAAKTYAKLPATMKEYVNF
                     INSYLGVNIQYISNGPGRDQLVKVG"
     assembly_gap    10511..10520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10521..10880
                     /locus_tag="PK977_04940"
     CDS             <10521..10880
                     /locus_tag="PK977_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17488.1"
                     /translation="AAAKKPSSKPTKKEEEEDDEDDFEDEDDVKPVKKGSKAASKKSD
                     EDDDEDIDDGPDDWEKPEEEEEWDPDFEEFDIPKSKGKKAAGKKGIDEDDDFKMDDEF
                     KDMFGDKDFDDDEEDDY"
     gene            10911..11909
                     /locus_tag="PK977_04945"
     CDS             10911..11909
                     /locus_tag="PK977_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRF17489.1"
                     /translation="MKNKSSTNCTFRVSDFEATKDKVLNWAKQFSTFCFLDNHQYQIE
                     PHSVECLLAAGQKRMISSIAGSALHELEQFLDERKSWLFGHLSYDLKNEIENISSRHV
                     DQVGFPDLFFFEPEILIRLSADEVCIEADDALAVFEKIENYVNNSLPVTGSFQVKNRI
                     SKSQYVDYVRQLQKHIARGDCYEVNYCQEFFAEGAIIDPLHVYKRLSETSPNPFSALY
                     RVHDKWLICASPERFLKRNSSSVISQPIKGTSRRAIDNVFTDELNKQTLYNSRKDRSE
                     NVMVVDLVRNDLAKVCREGTVKVDELFGIYSFPQVHQMISTVSGELKPQASFENLT"
     gene            11951..12223
                     /locus_tag="PK977_04950"
     CDS             11951..12223
                     /locus_tag="PK977_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRF17490.1"
                     /translation="MTGAPKRRVMELIEQYEQTKRGIFSGAVGYITPDGDFDFNVVIR
                     SIMYNETSKYLSFQAGSGITFYSEAEQEWEECLLKAEAIKRVLENY"
     assembly_gap    12258..12277
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12278..13301)
                     /locus_tag="PK977_04955"
     CDS             complement(<12278..13301)
                     /locus_tag="PK977_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRF17491.1"
                     /translation="MRKIVTITITGLTGFLLIQAAKATGIPHNQPLKNIAVADTAISD
                     SANRNTALINDPKLGFKNLFTTAVTGDGINSARLNPMAISFVQNYIARNSKGFKAMKD
                     WAKPYFDMMDNVLTQHGLPKELKYIAVIESGLKHNAISWAGACGPWAFMPAAARQYGL
                     DITRGRDERLDYYKSTHAAARLLTDLYAKYGDWLLVIAAYNGGPGNVDKAIRRAGGSK
                     DFWTLQYYLPNESMNHVKKFIGTHYIFEGEGGITTVTKNEMKDLVYEASARLKDEEIN
                     TSTAYNITGRFSSAVIMKYVNIDKPTFDRYNPGFDNKIAVDGKYELRLPTQMMNIFVA
                     KRYQILD"
     gene            complement(13330..>14572)
                     /gene="gatA"
                     /locus_tag="PK977_04960"
     CDS             complement(13330..>14572)
                     /gene="gatA"
                     /locus_tag="PK977_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRF17492.1"
                     /translation="GTPIGKLHGVVIGLKDVICYKDHPVTAASGILKDFTSIYSATAV
                     ERLIKEEAIIIGNLNCDEFAMGSTNENSTYGKVLNALDETRVPGGSSGGSAVAVQAGL
                     CMVSLGSDTGGSVRQPADFCGIIGLKPTYGRISRYGLIAYASSFDQIGIFGNNVKDVA
                     LLLEVMAGPDEFDSTVSLKEVPAYTRQLSSDKKYRFAYFKEALDHPGIDPEISKAIAS
                     KIEELKSQGHIAEKVDFDLLDYIVPAYYVLTTAEASSNLSRYDGIKFGYRTANPEIEL
                     TEFYKQTRSAGFGKEVKRRIMLGTFVLSAGYYDAYFTRAQKVRKLLTDKIQLIFRDFD
                     FILLPTSPVTAFKTGEKMDDPIAMYLADIYTVMANLVGVPAISIPLFKHSNGMPFGLQ
                     VMANRFDEVSLLQVSNKLLQD"
BASE COUNT         4238 a         2874 c         3199 g         3971 t
ORIGIN      
        1 atatcaaaga caaaccattt tgtacaggct ggaatagcgg gttgacaata ctgggcatgc
       61 aggatggtac ccggaccaat gccatagcgg cacctggtaa aaatgccaca gtatggggta
      121 caaatgcaat ggattcctgt tggaggttca ttccaaacgg aggtgtaccc aggtataaat
      181 ctgcccagtt attggttaac ggaactgtag tagcaactgc tgataccagt acttcagccc
      241 cgggtgaact aaacctcaat ttcaataacg tttgccctac agtagatagt acggcatatg
      301 tactgagggt agcatataca aattgtaacg gaccagatga tgtcattttt accgatacag
      361 tttttgtaaa gaaactatca ccttctatca ccaatctggc aaaaacagat cctacctgta
      421 cgccagacgg aacaataagt gtaactggtg caggtggtac acctcctttg agttacagta
      481 tcaatggtgg aactacttac caggcttcca atgtatttac cggtcttagt ggcggaacct
      541 atactgtaat tatcagggat ggattaggct gtatcagtgc tatacaaaca ataacgttag
      601 cgccggttaa ttcacttacc cagaccgtta ccaagacaga cgccaattgc acaacgtctg
      661 gttcaattac tattaacgca tctggcggtg gcacacctcc ttatcagtac agcattaatg
      721 gtggtagtac ctaccagtca tccaatgtct ttacaggact tgcagccggc acatacactg
      781 ttattacccg aaacccggta acgcagtgta ccgctactca gcaggtaacg attacattca
      841 ccaacaatct taccatgaac aatattgccg gaggtactat ttgccgtggt gcatccttca
      901 ctcctcaggt tagcagcaat gcagcttcct attcatggtc accttctgca ggggtaagca
      961 ataccactat cgctaacccg gtattatcgc cacaaaacac tactacgtat acggttaccg
     1021 gtactttggg aagctgcact attcagcgca ctgttactgt aacagttttt gaaggagcta
     1081 ctgccaatgc agggccggat gcaattattc tgacgggtga tgtttaccag atgttgtcgt
     1141 ctggaacagc tggtagttac ctttggacac cttccacagg attgagttca gctactgtgc
     1201 ttagtcctac tgctgctccg caaacaacga ctacttatac acttactgtt acctcaccac
     1261 aaggttgtac ggcaaccgac cagatgacac tgacagtagt gccctattgt attaagccga
     1321 tgaatgcctt cacgcctaat ggtgatggta ttaatgacag gtggctgatt acggacggta
     1381 actgcctgat cggcgtaagg gcacaggtgt ttaaccgata tggatcaaga gtatttgaat
     1441 cacccaatta cgataataca tgggatggta cttataaagg aaagcctttg ccagatggca
     1501 cttattatta tgtgctcgaa tttgtattac taaatggtaa acgccaggtg ttgaaaggtg
     1561 atgtgaccat tctacgttaa cagaaaaaaa tagaaaaatg aaaaagttat tattaataat
     1621 aaatatagta atgcttgcag gtgcggtgca aggacagcaa ctaatgacct catcgctcta
     1681 tgaccagcac gggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacaaatg ccaaaatagg tattggtggt
     1861 tatctgtata atgatgtaac aggccctacc cggcgcaccg gtggtcagat ggcttattct
     1921 tatcatatcc agatggccaa tgatgccact ttttcattag gactggaagc aaggttgcaa
     1981 cagttttcgc tggacaaagc aaagctttca gctacgttgg gtaatgatcc ggtactggcc
     2041 agttcagaga gccggttcaa agcagatgcc ggttttggca ttgcttacaa cggtaaaaaa
     2101 ttgcaggtag gtgcctccgt cagccagttg attcagtcaa aacttgattt ttattccggt
     2161 aacctgaacc cgacagaaga aggacgattg tacaggcatt actacctgca cggaagctat
     2221 aaatgggatg tggatggtag taccaggata attcctaacc tgcttttcat ttacctgcct
     2281 aatgcaccct tggaagtaca gggaggagcg agggttgagc ataatgatgt attctggtgg
     2341 ggacttgggt taagggctcg tcaaagctgg atgatctctg ctggtgtaaa gattcagaag
     2401 aaattcacca tcggttactg ttttgatatt ttcaatacgc cgcttagtgt ctatgatcgt
     2461 ggagccaacg gtcatgaaat attgctgcgc tacgattttc tcaagtaggc caggtactta
     2521 aaaaatttta aaggaggtaa tgatttttat taactcatta cctcctttct gtttctctct
     2581 ttttaactct tggctaaaaa ttgtaaattc gtagacaatg gatactgttg cgcagacacc
     2641 aaaatatcat ttgaatgaag aagaggagaa gaaactgatc ctccgggagt accgttcact
     2701 gctgaaagta ataaagaaca aattaaagcc cggtgacaag aacctgatcc gtacagcttt
     2761 tgaaatggca gcagaggcgc acaagaccat gcgccggaaa agcggcgagc cttatatcct
     2821 tcatcccatt gctgtagcta aaatttgtgt ggaagaaatc ggcctgggtg ttcgctctac
     2881 tatttgtgct ttactgcatg acacagtcga agacaccgat atttcactgg atgatgtaag
     2941 aagggaattt ggggaagaga tcaccaaaat tgtggacggt cttactaaaa ttgccaatgt
     3001 aatagatgtc aatgcatcgc agcaggcaga gaactttaaa aagatattgc tgacacttac
     3061 ggatgatccc cgtgtaattc tcatcaaact ggccgaccgc cttcataaca tgcggacact
     3121 ggacagcatg aagcgggaaa agcaattaaa gatttcttcg gaaaccgttt acatctatgc
     3181 accattggct catcgaatgg ggctgtacac cattaaaacg gaaatggaag acctggccat
     3241 gaaatatatg gagccggatg tgtacaagga tatagcccgc aaactggcag aaacaaaaaa
     3301 ggaacgaacc aagtatatca atgaattcat caagccaata aaagacaaga ttgtcaaagc
     3361 taacctggaa ggagatatct atggcaggcc aaagagtata cattccatct ggaataaaat
     3421 gaaaaagaaa ggtgtcgagt ttgaagaagt gtacgacctt tttgccatac gggtcattct
     3481 cgattcaccc ccggaaaagg aaaaagagga ttgctggaaa gtgtattcca tgatcacaga
     3541 tgaatacaat ccctcaccgg aaagattacg ggactggctg agtaacccca aagcaaatgg
     3601 gtacgaggcc ttgcacacta cggtaatggg cccgcagggt aaatgggtag aagtgcagat
     3661 acgtaccagg cgaatgaatg aaatagctga aaaaggcctg gccgctcact ggaaatacaa
     3721 agaagggaca agtgatgaaa gccggtttga taagtggttt caccagataa gagaggtaat
     3781 ccataaccag gaaacagata gtattgactt cctgcaggat ttcaagacca gttttctcac
     3841 agaggaaata tatgtgtaca ctcccaaagg agatgtaaaa atgctgccag tgggatcgac
     3901 agcgctggac tttgcctttg ctattcacag tgcaattggt gtaaaaacaa tcggtgccaa
     3961 ggtgaatcac aaactggttc cgatcagcca caagctgcat agcggcgacc aggtggaaat
     4021 tataaccagt aataaacaga aagcatcaga agactggttg ggttttgttg ttacttctaa
     4081 agcaaaagga agaatcaggg attcactaaa ggaagaaaaa agaaaagtat cagatgaggg
     4141 ccgctatacg gtacagcgaa aactggaagg gttgggtgcc gctatgtcgc agcataatat
     4201 tgatgagctg gtacactggt ataagctgcc atccaatttg gatctttttt accaggtagc
     4261 catcaagaat attgatctga aagagctaaa agacttcaaa gtcattggtg accgtattga
     4321 agcgccgaaa ccggtaaaac atgttaccga aaagcatgaa ttcattccgc atattggcaa
     4381 aaaagatgca gagctgatca ttttcggtga gagcagcgat aagattgtat ataaccttgc
     4441 caaatgttgt aaccctattc cgggtgatga tgtgtttgga tttgtaacaa caggtaaggg
     4501 cctaaccatt catcgttcca attgtcccaa tgcggccaag ttactagcca gttatggaca
     4561 cagggttgta aaaaccaaat gggccaagaa taaagaaata tccttcctga caggaataaa
     4621 aatagtgggc atggatgatg tgggggtcgt tagtaaaatt accaacctta tatccggaga
     4681 tttgaagatc aatatttctg ccctgaccat tgaggcgaag gagggtttgt ttgaaggaaa
     4741 tattaagctc tatgtacatg ataaagaaga gcttgacaat ttagtgcaga agttgcttgc
     4801 tttaccgggt attgaatcgg tagaacggta tgatatggag gatattcctt cctagatttt
     4861 tgttcacaat caagctattt tgatggtcct tagcctgatt tttgtttatc ttaacggctt
     4921 gttaaggact gtatactttc acatactgca tttatttatg agatctatcc ggctgcgtat
     4981 tttttcccat attattttag gggtcataac tcttttttct acagttactc attacggcca
     5041 aagttgtttg cctaccaata ttaatggtac gaatatcaac ctgctttgta accagatttg
     5101 tactactctg gttttcaggg ttccccattt aaaaagttct gatgattatg cagtcaacac
     5161 tataacatat gctccttttg cctatacaac tccaacagga aatgaacttc cgactttata
     5221 tgcagatgat gagtatggac cattagtaaa cctgcctttt tctttttgtt tttatggagc
     5281 gaattataat agtgtagtgg tagggtcaaa cagtattata acctttgatg ccagtactgc
     5341 taatcaggca aatgcatacc cgataactca gccaatacca tatgcaggtg gaaccccgaa
     5401 cgtaatatct ccggcctatt atccgccagc ttctattatg gctggttatt ctgatattga
     5461 tccaagggca agtcaatcac ctcctgatag aaaaattgag tggagaattg aaggaacagc
     5521 tccttgtcgc cggtttattg caagttattg gcatgttgga acttttggtg gaggggtctg
     5581 gtctgcaact ggaactaact gtaactccca aaaccccaac aatattcaaa ttgtcattta
     5641 cgagtcaact ggtataattg acatttttat tggaaataaa atttgtattc cgagtacaaa
     5701 tggaggcaac tcaattctcg gaattcaaaa ttgggccagg aatgctgcga gatgggcgcc
     5761 gggaaaaaat gcggtacagt ggacttcaac gaatgaaggt tatcgcttta cgccagccgg
     5821 gggcacatcg aaatttatct cttcacagct gtttacactt acaggcaccc ttgtcgccac
     5881 agcagacaca tctacaacta cagcgggttt attagatatt agttttccta acatctgtgc
     5941 tcctgcaggt ccaacccaat atgaagttgt tacaactttt tctgcttgtg ataatcctgc
     6001 aaacctttta gttagccggg ataccattac tctaaataga acaaatacac ttggcgctac
     6061 cagttcattt actaatacta cctgcggccc tccatcagga actattacag ttacaatacc
     6121 tgctggtgtg ggtaccgctc ctttttctta tgtattggat ggagggactc ctgttgtaac
     6181 accagctttg acttatacgt ttacaggtgt tgctgctggc ccacatactg tagttgtaac
     6241 tgatgcttcc acaggttgtt ccagtactat aaacgtaaat attacacann nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnacaaat ggctctgcac catatagcta tgtacttgat ggaggtgtac
     6421 cagttagcgg tggctcacct tatactttta ctgggctttc tgctggtaac catacaatag
     6481 taattacaga tgccagcggc tgtatttcaa atacaataac tgttaatgtt gccactggtt
     6541 caggtgtaaa tgtttctacg acatcaactg ccaccagttg cccgggtgct tcgaatggaa
     6601 caataactgt taatgcttct ggtggtgtac ctgcatatac gtatcaatta ggtgccaatc
     6661 ctccgcaggc atctaatgta tttaccgggt tagcttccgg aacctatact attaccgtaa
     6721 gggataatgt gggttgtata aggactgtat caagaactgt aactgcggga tcggccattg
     6781 tcgccagtat gaacactaca ggaaccacct gttcaggtgc ggccaatgga agcgttacgg
     6841 tgacgccaac taacggaacc ggtccgtata catttgtatt ggatggtact gttacgcaaa
     6901 ccggtgctac taatacaacc tttactaatc tttcagcagg tcctcataca attattatta
     6961 cagataatcc cagcggatgt ctgagcaata cgatcaatat caacatcccg gcaggcccgg
     7021 cattggctgc cagtgttact tcgtctgcga caacctgtag cggagcaagt aatggtacta
     7081 ttacagtaac acccacaaac ggtacaggac cgtatgagtt ctcattagat ggcggtccgt
     7141 tggtctcggg tgcagtgcct tatattttta ccaatgttcc tgccggaagt catactattc
     7201 aggtaacaga tattcccaca ggttgtatct ctactattta tacaactttg gtgagtgccg
     7261 gtcctgtatt gctgacaact gccagcaaaa ccgatgtatt atgtaatggt ggagctaccg
     7321 gcacaataac agttgcacag ccagccgccg gcactgcacc gtatgagtat tcactggata
     7381 atgtgaactg gcaaagcagc ccggtattta ctggtttgac agcaggaact tacactgttt
     7441 actataggga aagcaatggt tgttcaaata gccttagcat aacggtaaat gaaccgtcaa
     7501 cactgactgc tacagatgca tccactgctg ttatttgtaa tggagaaacc aatggtacca
     7561 taactgttac agccaatgga ggtgtttcac cctatgatta ttctattgac ggaggtgcta
     7621 actggcagac gggtaatatt ttcaccgtag cggcaggtgc ctacacggtg atcatcagag
     7681 acgcaaacgg ttgtactgtt tccagaaata taactgtgac ggctgtgtta acagcgaatt
     7741 cagccaatac aaatgccagt tgcgatggcg gcaatgacgg tcttattgat gtggctgcag
     7801 ttggaggaaa tggtaattac cagtattcaa taaatgggac aactttccag tcttctaata
     7861 ttttcaatgt agcgccaggt acttatactg tgactgttag agataacctg ggttgtacca
     7921 caagtttcag taccaccgtt ggtttgtcca gtaatattac atttactcca caggtggatc
     7981 ctactatttg tgaaggatct tccgcacaat tacaacttac ttccaatgcg acccaatact
     8041 cctggagtcc cgctgtcgca ttaagtgata ccactattta taacccggtg gctaaccctg
     8101 ttgtaactac acaatacatt gtaactgcta cattgggtcg ttgcagtatt attgacacgg
     8161 taattgttaa tgtgaaccct gccccggtgc ctgatgccgg accagccgga tatatctgtt
     8221 acggtcaaac ctaccagttg caggcatctg gcggtgtgca gtatcaatgg acaccggcta
     8281 catacctgaa caatcccaac attgctaatc cggtatcatc tgctccgcag gatattacat
     8341 acacagtatc gattattgct gatgccaatg gttgtccttc tttaactaca gatgatatta
     8401 ctattgatgt aaccccgccc ataaagatca atactttccc atacgatact atcggttatc
     8461 ccggagatca gatacaatta ttagcgattc ctacagatac tgatgctact actattgtat
     8521 ggacaccttc aacggggctt gataatcctt ttgtgcccaa ccccatcgtt acgattggcc
     8581 aggtaggaga tgacagggtt taccaggtta cggcatcaac nnnnnnnnnn nnnnnnnnng
     8641 tgcgggttcg ggtatataaa ggtcctgata tttatgttcc ttcaggcttt acaccaaatg
     8701 gtgacggaag aaatgaccgc tttacaccat tccctgtagg tataaagtcc ttaacttatt
     8761 tcagaatatt caatcgctgg gggcagcaga tattttcaac caacagattg catgacggct
     8821 gggatggtac gttgaatggc actgcacagc cttccggagt ctatgtatgg atgattcagg
     8881 cagttacaga tgacaaccgt gtgatcaaca aaaaaggaac agttactata atccgataat
     8941 taattggtga taactcagct atttgggtat aaaaaactgg caaagccacc ggatttaatt
     9001 cggtggcttt ttagtgaacc cgtaattttg ccctgccttt gcaaaagcta tggcaggcgg
     9061 gcccgcccat ttcaaaaggg caaagaaaaa tatnnnnnnn nnnnnnnnnn attacttggc
     9121 ctccagtggg gggatgaagg aaaaggaaag attgtagatt tttttgcgaa agaatatgac
     9181 ctggtagccc gtttccaggg tggtcccaat gcaggtcata ccttgtatgt taatgataaa
     9241 aaagtagtgt tgcaccagat accttccggc gtttttcatg aagggataat aaacctgata
     9301 ggtaatggtg tggtgcttga tcctgtgaca ttgaaaagag aatgtgatac cgtagcatct
     9361 tttggaatcg atctcaggaa aaatcttttt atagcagaaa gaactcacct tattttacct
     9421 acacaccgtg ccctggataa agcagctgaa ttatcaaaag gtcaacaaaa gatcggatca
     9481 accttaaaag gcataggccc tgcttatatg gataaaaccg gccgtaatgg tctaagggtg
     9541 ggagatttac tggataaaaa ttttacaact tcgtatatca agctgcgctt aaagcatcag
     9601 cgtttgctgg ataattataa ctttcaggaa gatatttcgc aatgggaaga ggagtttttt
     9661 gaagcggtgg aatttcttaa acagctgaat attgttaatg gcgaatattt tattaacagt
     9721 aacctgaaag caggtaagaa gatcctggct gaaggggccc agggaagtat gctggatatt
     9781 gactttggta ctttcccgtt tgttacttca tccaatacca tttctgccgg tgtatgcagt
     9841 ggtttaggaa tagcaccggg tcatatcagg gatgtgattg gggtgacaaa ggcttactgc
     9901 acccgggtag gcggtggtcc ttttccaact gaattggaaa atgaaaccgg agaagagtta
     9961 agaaagattg gaaatgaatt tggtgctacc accggccgtc cccgtcgttg cggctggatt
    10021 gatcttgtag ctttgaattt tgcctgtatg atcaatgggg tcactaaact ggtgatgaca
    10081 aaagcagatg tattggatgc gtttaaggag ttgcagcttt gtactgatta caaaataaac
    10141 ggaaaacaga caagggagat accatttcag atggaaagac aaaaaccaga accggtttac
    10201 aagcagtttg ccggctggaa tacagctact gatgcagcga aaacctatgc gaaattgcct
    10261 gcaactatga aagaatatgt aaactttatc aatagttact taggggtcaa tatacaatac
    10321 atttctaatg gcccgggaag agatcagttg gtaaaagttg gataataaac aagcaaggaa
    10381 aaaatatttt taaaaatttg gccaattaaa aagttcgccg aaattttaca gctataatcc
    10441 tgaaacgact atggcaataa aaccagaaaa aggaaaaaag actcccggga gaacttcagc
    10501 tgcctccgaa nnnnnnnnnn gctgctgcaa aaaaaccgtc ttcaaagcca acaaagaaag
    10561 aggaggaaga agatgatgaa gacgattttg aagatgagga tgatgtgaag cctgtaaaaa
    10621 agggtagtaa agctgcttct aagaagtcag atgaagacga tgatgaagat attgatgatg
    10681 gtcctgacga ttgggagaaa ccggaagaag aggaagaatg ggatcctgac ttcgaggagt
    10741 ttgatattcc caagtcaaaa gggaaaaaag ccgctggaaa aaaagggatt gatgaagacg
    10801 atgattttaa gatggatgat gagtttaagg atatgtttgg tgataaggat tttgacgatg
    10861 atgaagagga tgattattaa tttagccgct ttgcggggta acttatcaat ttgaaaaata
    10921 aatcctcgac taactgcaca tttcgtgtaa gcgactttga ggcgacaaaa gataaagtgt
    10981 tgaactgggc taaacagttc agcacttttt gttttttgga taaccaccag taccagattg
    11041 aaccgcacag tgtagaatgc ttattggcag ctggccaaaa gaggatgatc agttcaattg
    11101 ccggttcagc tttacatgaa ctagagcagt ttttggatga aagaaaaagc tggctttttg
    11161 gtcatctttc gtatgatctt aagaacgaaa ttgaaaacat ctcttcccgt catgttgacc
    11221 aggtagggtt tccagacctc tttttttttg aaccggaaat tttaattcgt ctctctgctg
    11281 atgaagtatg tattgaagca gacgatgctt tagcagtatt tgaaaagata gagaactatg
    11341 taaataattc tttaccggta accggctctt tccaggtaaa aaacagaatt tctaaaagtc
    11401 aatatgtgga ttatgttcgc cagttgcaga aacatattgc aaggggtgat tgttatgaag
    11461 tgaattactg ccaggagttt tttgcagaag gggcaattat cgaccctttg catgtttaca
    11521 aaagactatc ggagacatca cctaatcctt tttctgcttt ataccgggtg catgataaat
    11581 ggcttatttg tgcaagtccg gaacggtttc tcaaaagaaa cagcagttct gttatctccc
    11641 agccaataaa aggcacatcc cggagagcca ttgataatgt ttttacggat gagcttaaca
    11701 aacaaacatt gtacaacagc agaaaagaca ggtctgaaaa tgtgatggtg gttgacctgg
    11761 ttagaaatga cctggcaaaa gtttgccggg aaggtactgt aaaggttgat gagctgtttg
    11821 gtatttattc ttttccgcag gtgcatcaga tgatatcaac tgttagtggt gaattaaagc
    11881 cccaagccag ttttgaaaac cttacataag atttgaagaa ataataaggg ctgcatttcc
    11941 tatgggctca atgaccggag cccctaaaag aagagtgatg gaattgatag aacagtatga
    12001 gcagactaag cggggaatct tctccggggc tgttggttat ataaccccag atggcgattt
    12061 tgacttcaat gtggtgatca ggagtatcat gtacaatgaa acatcaaaat acctttcttt
    12121 ccaggcgggt tccggaatta ctttttacag tgaagctgaa caagaatggg aagagtgctt
    12181 gctaaaggcc gaagctatta agagagtact ggaaaattac tagcgggagg cattatcaac
    12241 tggtttcagc agtagttnnn nnnnnnnnnn nnnnnnncgt ctaatatctg gtagcgtttt
    12301 gctacaaata tgttcatcat ttgcgtgggt agtcgcagtt catattttcc gtcaacggct
    12361 atcttattat caaatccagg gttatacctg tcgaatgttg gcttatcaat atttacatat
    12421 ttcatgatga cagcagaact aaatctgcca gtaatattat atgctgttga agtattaatt
    12481 tcttcatcct ttaaacgggc tgatgcctca taaacgagat ctttcatctc atttttagtt
    12541 acagtggtga ttccgccttc cccttcaaat atgtagtgag tgccaatgaa tttttttaca
    12601 tgattcatgg attcgttggg aaggtaatat tgtaatgtcc agaaatcttt gctgcctccg
    12661 gcacgacgta tggctttgtc tacattgcct ggaccaccat tgtatgcagc tattacaagc
    12721 aaccaatccc catattttgc atataggtcc gtcaacaacc ttgctgcggc atgggtgctt
    12781 ttgtaataat caagtcgttc gtctcggccc cgagtgatat ccagtccgta ttggcgggct
    12841 gcagccggca taaatgccca ggggccacag gcgcctgccc aagaaatggc attgtgcttc
    12901 aggccggact ctataacggc tatgtatttt aattcttttg gaaggccatg ttgagtcaaa
    12961 acattgtcca tcatgtcaaa atacggttta gcccaatcct tcatagcttt aaacccttta
    13021 ctatttctgg caatatagtt ttggacaaag ctgatggcca ttggatttag tctcgcactg
    13081 ttgattccat caccagttac agctgtcgta aaaagattct tgaaaccaag tttagggtcg
    13141 tttatgagag cggtattccg atttgcagaa tcgcttatgg cagtatccgc cacagcaatg
    13201 ttttttaatg gttggttatg tggaatacca gtagcttttg ccgcctgtat cagcaaaaat
    13261 ccggttaacc cggtaattgt tatggttaca atttttctca agacaacaga ttttcaatgt
    13321 tttacagttt tagtcctgta aaagtttgtt ggaaacctgc agcaaagata cttcatcaaa
    13381 gcggtttgcc attacctgga gaccgaaggg catgccattg ctgtgcttga aaagggggat
    13441 tgaaatggcc gggacaccca ctaaatttgc cattacggta taaatatcag cgagatacat
    13501 ggcaatgggg tcgtccattt tttcaccggt tttaaaggca gttaccggtg aagtagggag
    13561 taaaatgaag tcaaagtccc tgaaaatcaa ctgaatcttg tctgttaata actttcttac
    13621 cttctgtgcc cgggtaaagt aggcatcata gtaaccagca cttaacacga aggtgcccag
    13681 catgatacgg cgtttgactt ctttaccaaa accagctgac cgggtctgct tataaaactc
    13741 tgttaattca atttccgggt tggcagtacg gtagccaaac tttatcccgt cataacgtga
    13801 aagattggaa gaggcctctg cagtagttaa cacataatag gcaggaacaa tgtaatcgag
    13861 caggtcaaaa tccacttttt cagcaatatg tccctgactt ttcaattcct caatcttgct
    13921 ggcaattgcc ttacttatct caggatcaat accaggatgg tcaagggctt ctttgaaata
    13981 agcaaaacga tactttttgt cggaagaaag ctggcgagta taggcaggta cctcttttaa
    14041 tgaaactgta ctgtcaaatt cgtcaggtcc ggccataact tccagcaaca aggctacatc
    14101 tttgacattg tttccaaaaa tgccaatctg gtcaaaagat gaagcataag caatgagtcc
    14161 ataccgggag attctaccat atgttggctt caagccaata attccacaga aatctgctgg
    14221 ttgccttaca gaaccgcctg tatcacttcc taaagaaacc atacataaac cagcttgcac
    14281 agcaacagcg gaacctccgg atgatccacc gggcacccgg gtctcatcca atgcatttag
    14341 tactttacca taggtagaat tttcgttggt actacccatg gcaaattcat cacagttcag
    14401 gttaccaatg attatggctt cttccttaat taatcgctct acggcggtag cagaataaat
    14461 ggatgtaaaa tcttttaata ttccagacgc agcagttacg ggatgatcct tatagcagat
    14521 cacatctttt aatccaataa ctactccatg cagtttacca ataggagttc ct
//
LOCUS       DAORLS010000041        14377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2261_length_14377_cov_1.986175, whole genome shotgun sequence.
ACCESSION   DAORLS010000041 DAORLS010000000
VERSION     DAORLS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14377
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_14377_cov_1.986175"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /gene="ppk1"
                     /locus_tag="PK977_04965"
     CDS             <1..1574
                     /gene="ppk1"
                     /locus_tag="PK977_04965"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRF17493.1"
                     /translation="ALIEIPSRTLGRFTILPSPKPDENHIILLEDVIRFNLPEIFSYF
                     GYDQYQSHIFKVTRDAEIDIDEDISTSIIQKLEKGIKNRRKGKPVRFVYDREMDPGLL
                     EYLVRRLNLSKRDNLIPGGRIHNFRHFMDFPDQVFKEKRLRKKPFDHPLLTVRRVTDV
                     IMEKDVLLNFPYHSFLPLIDLLREAAFDPDVSSIKITCYRLAPQSKIINALINAVRNG
                     KEVTVMLELRARFEEEANLEWKERLEEEGAKVLVGIPNMKVHAKVCVIKKRTKDHNFF
                     YYGFVSTGNLSERTAKVYADHCLLTSNQKIMADVNRIFNFLEQPKIGMNVLKECKAII
                     PSPFFVKREMIRLIDEEIKQARKKKPAYITLKMNSLSDEEMIQKLYEAARAGVQIKLI
                     VRGIFCMLSENEKFRKPVKAISIVDEYLEHARVWVFHNKGKEKVYISSADWMVRNLEH
                     RVEATCPIWHEELKQELIDILDIQLQDNVKARWLNNELDNEYVRTTKKKIRSQVETYS
                     YLFNKTKTNRETGSH"
     gene            1555..2451
                     /locus_tag="PK977_04970"
     CDS             1555..2451
                     /locus_tag="PK977_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRF17494.1"
                     /translation="MRLAAIDIGSNAARLLITDVIAGPQGVNEFIKVVLVRVPLRLGF
                     DVFDKGEISDGKVEKIIKTIKSYKLLIDVYEVKHLKACATSAMRDAANSPEIIKKVKA
                     ETGIDIEIISGDQEASLIYENHIAENLTNDESYLYIDVGGGSTELTFFSDGKLVFKKS
                     FNIGTIRLLKNQVTEAAWDELKEFIRNKTKGYHHVTAIGSGGNINKIFSISKRKEGKP
                     LQLDLLRDYYKEFSNLSLSQRISLHKLREDRADVIVPALLIYINVMRWADANEIFVPK
                     IGLADGLIHTLYEEVKLKDMEI"
     gene            2479..3294
                     /gene="panB"
                     /locus_tag="PK977_04975"
     CDS             2479..3294
                     /gene="panB"
                     /locus_tag="PK977_04975"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRF17495.1"
                     /translation="MSVNKEVKRVTTNTLQKMKNAGEKISMLTAYDFSFAKIIDSAGI
                     DVILVGDSASNVMAGHETTLPITLDQMIYHASSVIRGVERCLVVVDLPFGSYQSNSDI
                     ALASAIRIMKETGAHSVKMEGGEEVIDSIRKIVSAGIPVMGHLGLIPQSIYKFGTYAV
                     RAKGGTEADKLRRDAKLLEEAGCFAIVLEKIPAELAKEVSESLTIPTIGIGAGGDCDG
                     QVLVMHDMLGINTEFKPRFLRQYLNIHELAVKGVQQYISDVKSGDFPNDQEQY"
     gene            3331..4248
                     /locus_tag="PK977_04980"
     CDS             3331..4248
                     /locus_tag="PK977_04980"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HRF17496.1"
                     /translation="MKQQLFLFFTAITTLFLFQSCQKEISFDDDNNTVTGDFRAKIEG
                     SQWVANKGAGASRMQGLINISGYSTDKKYITITLADSGVHRYTLSDATMNAAAYIDST
                     LPNPIAFTTNQGTYPTESGGEVNITSIDTANKKMSGTFSFKVFRMMDGLGRNITEGSF
                     TNLSYTTTLPPANATDTFKVKISGTQWNPPTIFAVKAPAVPPLPATIAVSGTDATASK
                     VVGLIFPADITPGSYTLDFFGGTYIGQYNPNSNPQNSQASVSGTLQIISHNTSTKRIR
                     GNFNFRSEELLNPLAFTLLTEGYFSVTYH"
     assembly_gap    4288..4297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4380..6302
                     /locus_tag="PK977_04985"
     CDS             4380..6302
                     /locus_tag="PK977_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF17497.1"
                     /translation="MSIAKRAFFAVMLLTVSHIIVAQDSTLQLDNVIITATLSAQKQK
                     ESGRNIVAIKGESFSKLPVHSVDDLLRYLPGIEVQQRGPQGSQSDIVIRGGTFQQVLV
                     IIDGVKLNDPLTGHFSMYVPIHSSEIERVEILKGAASAIWGSEAVGGVVNIITKTFSN
                     SKNKNQIKAKTVAGEYGLFNGDAYVSYSKNKSAFAAGILSNNANGQPLRGTNGFFHLT
                     TANISFSQGLAKDWRLNFRTAADLRKFNAQNFYTTFNSDTAEEKVNTWWNHLNLQKKT
                     NRGTLNFDAAYKKLRDQYWFRPASVPNDNRTSLFTSQLYYSADINKKHGYTGGLQVHR
                     KEIRSNDRGNHSLWHAAAYSIFRHKLKNGIYLNESLRLDWDESYGWILSPQLNIAWAP
                     SKLTLRSSVGRSFRDADFTERYNNYNKELVSSGRIGNPDLEAEQSWNAEIGIDYNFSA
                     RFRISGTIFYRDHSNLIDWANTPYAEMPRKSNLSPAGNYALAKNVEEVRTRGMEIDII
                     YNEKLGQNASLLATAGLTWLKSVNDDPVPSFYISSHARFMTNFSLAYTIHSFSVSVNG
                     VYKQRDEQKSSAINAEISPSYFVLNTKAAYSTKNGWGKCFIQADNIFNKGYSDLLGAV
                     MPGRWLSGGIEIALPY"
     gene            6312..>7458
                     /locus_tag="PK977_04990"
     CDS             6312..>7458
                     /locus_tag="PK977_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF17498.1"
                     /translation="MDFLITLKIDSTNKGVSTGTQWINSKGEIIDSFSPVDGKKIGSV
                     TAADKESYEAVIKKAEEAFKTWRLVPAPKRGEIVRQIGEALRQYKEPLGKLVSYEMGK
                     SLQEGLGEVQEMIDICDFAVGLSRQLHGLTMHSERPGHRMYEQYHPLGIVGIISAFNF
                     PVAVWSWNSMLAWVCGDVCVWKPSEKVPLCGIACQHIITEVFKKNNVPEGVSGLIVGG
                     REVGEWMAADSRIPLVSATGSTRMGKAVGATVAARLGRSLLELGGNNAIIISKEADLD
                     IAILGALFGAVGTAGQRCTTTRRLIIHEDVYDTVKNKLVSAYKQLRIGDPLDQKNHVG
                     PLIDKDAVRMYLDSIEQCKAEGGNFIVEGGVLSGPGYESGCYVKPCIA"
     assembly_gap    7459..7485
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7486..7852
                     /locus_tag="PK977_04995"
     CDS             <7486..7852
                     /locus_tag="PK977_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF17499.1"
                     /translation="DYKIVQHETFAPILYLLKYKTIEEAIAIQNEVPQGLSSSIMTLN
                     MREAEQFLSHAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQT
                     NTINYSTSLPLAQGIKFDV"
     gene            8021..9715
                     /locus_tag="PK977_05000"
     CDS             8021..9715
                     /locus_tag="PK977_05000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRF17500.1"
                     /translation="MNRTVKRTSILFFSGLISTAALAQKDEIPKGWHMMDKAASGYHG
                     ISIDKAYEFVNSKKLKSNTVIVAVIDSGIDTLHEDLKPVLWKNAKEIPGNGIDDDKNG
                     YVDDVYGWNFLGGRDGKNVESDSYEAARVFHKFKSKYSGKTIDKSALSKEELFEYEMW
                     KRSEGEIAGGDSKSNALELIFLKRAYTNCVKADSILRKAMGKDVYTGKELGEFSSDDT
                     DVKKSKNTLYGLMSGNDALESTNKDFLEGFGDYVSGEEKKAQAAETAPTPYRADIVKD
                     NYDDINDKFYGNNNVMVSNKSALHGTHVSGIIAAARKNGVGMDGVADNVKIMTLRAVP
                     DGDEHDKDIALAIRYAVDNGAQVINMSFGKGFSPEKKWVDDAVKYAESKGVLLVHAAG
                     NDAKNLDTTFNYPTPQLLDGSRPSNWLTVGASGDPKAGGLTASFSNYGKNEVDVFAPG
                     VKIYSTVPGGNTYQNLQGTSMASPVVAGLAAFILQYYPTLSPAQVKMVIEKSAQSPGT
                     TVKNPGNDEMVDLSELSRAGGIVNAYEAVKLAASLKGERKPAVVKPATTVKPKARN"
     gene            9812..10516
                     /locus_tag="PK977_05005"
     CDS             9812..10516
                     /locus_tag="PK977_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17501.1"
                     /translation="MRNFLFGTVFFSTLLIAGCGSSDEESDETTNTTTTTAPPSAVTA
                     DTNQNKIALPATNTVTAPVLNNQPVVTSSAGINPEHGKPGHRCDIAVGAPLDSKPTQP
                     VANPSVSIPANGQKSAPVISAPTITTPVVTPTVTPTTTPVTGAGLNPEHGKPGHRCDI
                     AVGAPLNSAPATKTTQPAATITPSISPAPAVQPVATPVTPSVNTDGTALNPEHGKPGH
                     RCDIAVGAPLNSKPKQ"
     gene            10525..11034
                     /locus_tag="PK977_05010"
     CDS             10525..11034
                     /locus_tag="PK977_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF17502.1"
                     /translation="MGKPDLSRIPEWYHGYINKVKEDDLTAAFNIQTPPFLKFLEELP
                     EAKRDYRYADGKWTVKEMLQHIIDAERIFAYRALRFARKDRTPLPGFDENEYAKNALV
                     SNRDWDNMVEEFRVLRKSTELMFASFSNEELELSGTASEKPVYVLGIGFIIIGHVAHH
                     RDIIKERYL"
     gene            complement(11040..11384)
                     /locus_tag="PK977_05015"
     CDS             complement(11040..11384)
                     /locus_tag="PK977_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRF17503.1"
                     /translation="METTTIVPVRLTAGAVAEIRKLMNEPGFDATQKLRIGVKGGGCS
                     GMTYVLGFDQPKENDNTFEIDGISCIMDKSHELYLYGMEVDWQDGLNNRGFTFKNPNA
                     STTCGCGTSFAV"
     gene            11532..>11771
                     /locus_tag="PK977_05020"
     CDS             11532..>11771
                     /locus_tag="PK977_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17504.1"
                     /translation="MDLTVVSIILASLAVIISVIALFRKSDTPPLEPAGEKFDSTSLR
                     LQAYERLVLLAERISLPNLISRLNQPGIPATDMKII"
     assembly_gap    11772..11861
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12041..>12808
                     /locus_tag="PK977_05025"
     CDS             12041..>12808
                     /locus_tag="PK977_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HRF17505.1"
                     /translation="MADDNKKFTDSGIEIKPLYTSSDWSNPENELPGNYPFTRGVQAD
                     MYRGKLWTMRQYAGFSTAEESNKRYHYLLSQGVNGLSVAFDLPTQIGYDSDHHLAEGE
                     VGKVGVAIDSIEDMQILFNGIKLEEVSTSMTINATGFILLSLYAALAKQQGADLKKIS
                     GTIQNDILKEYAARGTYIYPPKPSMRIITDIFEWCSKEVPKWNTISISGYHIREAGST
                     AVQEIAFTLSNGKAYVQAALNKGLDINVFGKRLSFFFN"
     assembly_gap    12809..12843
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12844..>13217
                     /locus_tag="PK977_05030"
     CDS             <12844..>13217
                     /locus_tag="PK977_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF17506.1"
                     /translation="EEVAKFRAARRMWAKIMKELGATDPKAMMLRFHTQTGGSTLTAQ
                     QPLNNISRVTIQTLAAVLGGTQSLHTNGYDEALSLPTEEAARIALRTQQIVAFESGVT
                     ETVDPLAGSYFVESLTQQVEEA"
     assembly_gap    13218..13227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13228..13626
                     /locus_tag="PK977_05035"
     CDS             <13228..13626
                     /locus_tag="PK977_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF17507.1"
                     /translation="KLIEKIDAMGGSVTAIEQGFIQDEIARSAYEYQRNIEDSNKIIV
                     GVNKFQVASEDHIPILKVDDSIRTLQSAKLAKLRSNRDNAKVDQCLQELNDKAVSGDN
                     LMPAVLEAVESKCTLGEIADTLREVFGEYK"
     gene            13721..>14377
                     /locus_tag="PK977_05040"
     CDS             13721..>14377
                     /locus_tag="PK977_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17508.1"
                     /translation="MKPLTLTRALVCLTFLVLSTESFSQVKIGGNPKEDPHPSAVLEL
                     QGTDKGLLLPRLSQQQMHSIKQPANALVVYNTDAKNVFIYSEDRKEWMPLMQEAQRLG
                     EDSCEWEFDTSTLRVFLVRGYPLGDSVYYNTARRKFVFSDKINVEGSNTPADVFYPGK
                     YIFKGNASGVFHDSASLNFPSITLSNFLFAIDNDSFAVANPNLAFYNGMRISTQMLPS
                     A"
BASE COUNT         4538 a         2851 c         3057 g         3759 t
ORIGIN      
        1 atgcactgat cgaaattccc agccgtacgc tgggccgctt caccattctg ccttccccaa
       61 aacctgatga aaaccacatt atcctgcttg aagatgtaat ccgtttcaat ttacctgaga
      121 ttttttcata cttcggttat gatcagtacc agtcacatat tttcaaagta acccgggatg
      181 ctgaaattga tattgacgaa gatatctcca ccagcattat tcaaaaatta gaaaaaggta
      241 tcaagaaccg gcgaaaaggc aagcctgtaa gatttgttta cgaccgggaa atggaccccg
      301 gcttgctgga atacctcgtc agaagattaa atctttccaa aagagataac ctcattcccg
      361 gtggccgtat tcataatttc cgtcacttta tggattttcc cgaccaggta ttcaaagaaa
      421 aaagactccg gaaaaaacct tttgatcatc cgctgttaac ggtacggaga gttactgatg
      481 tgattatgga aaaagatgta ttgctcaact ttccttatca ttcttttctt ccgttgatcg
      541 acttattgcg ggaagcagca tttgatcccg atgtgagcag tattaaaatt acctgttacc
      601 gcctggcgcc gcagtccaag attataaatg cactgatcaa tgcagttcgc aatggaaaag
      661 aagtaacggt aatgctggag ttacgggcaa ggtttgaaga agaagcaaac ctggaatgga
      721 aagagagact ggaagaagaa ggcgcaaaag tgctggtagg tatacccaat atgaaagtac
      781 atgccaaggt ttgtgtgata aaaaagagaa caaaggatca caacttcttt tattatggtt
      841 ttgtgagtac cggcaatctc agcgaaagaa cggcgaaagt atacgccgat cactgcctgt
      901 taacttccaa tcaaaaaata atggcggatg tgaaccggat cttcaacttt cttgaacaac
      961 ccaaaatagg catgaatgtt ctgaaagaat gtaaagctat catacccagc ccgttttttg
     1021 ttaaaaggga aatgatccgg ctgattgatg aagagatcaa acaggccaga aagaaaaaac
     1081 cggcgtacat aacacttaaa atgaattcac tttctgatga agaaatgatt cagaaattgt
     1141 acgaagcggc aagggccggc gtacaaataa aattaatagt gaggggtatt ttctgcatgc
     1201 tttcagaaaa tgaaaaattc cgaaagcctg ttaaagctat aagtattgtg gacgaatacc
     1261 ttgaacatgc aagggtgtgg gtatttcaca acaaaggcaa agagaaagtt tatatatctt
     1321 cggccgactg gatggttcga aatcttgaac acagggttga agccacttgc ccgatatggc
     1381 acgaagaact gaaacaggaa ctgattgaca tacttgatat tcaattgcag gacaatgtca
     1441 aggcaagatg gttgaacaac gaactggata acgaatatgt cagaaccacc aaaaagaaaa
     1501 taagatcaca ggtagaaacc tatagctacc tcttcaataa aacaaagaca aaccgtgaga
     1561 ctggcagcca ttgatattgg aagtaacgcc gcaagattat tgatcacaga tgttatagca
     1621 ggaccacagg gagtaaatga attcatcaaa gtagtactgg tcagggtgcc cctgcgtcta
     1681 ggtttcgatg tttttgataa aggtgaaatt tccgatggta aagttgagaa gattattaaa
     1741 actatcaagt cttataaact tctgatcgat gtctatgaag taaaacacct gaaggcatgc
     1801 gccacatccg ccatgcgtga tgctgccaat tctccggaga taataaaaaa ggtgaaagca
     1861 gaaacaggta ttgatattga gatcatcagt ggcgaccagg aagcttcatt gatatatgaa
     1921 aaccatatag ccgagaattt aaccaacgac gaatcttatc tgtatattga tgtagggggc
     1981 ggcagcacag aacttacttt tttcagcgat ggcaaactgg tttttaagaa atcattcaat
     2041 ataggcacta tccgattgct gaagaaccag gttaccgaag cagcctggga tgaattaaaa
     2101 gaattcatcc gcaacaaaac aaaaggctat caccatgtaa cagcaatcgg ttctggaggg
     2161 aatattaata aaatattctc catttctaaa agaaaagaag gtaaaccact ccaacttgac
     2221 ctgctgagag attattacaa ggaattcagc aacctttctc tttctcaacg tatcagtctg
     2281 cataagttaa gagaagacag ggcagatgta attgtaccag ccttgctcat ctatatcaat
     2341 gtgatgcgtt gggccgatgc gaatgaaata tttgttccta aaatcggtct ggccgatgga
     2401 ttgatacata cactatacga agaggtgaag ctaaaagata tggagatctg accgaaaatc
     2461 atattcaaac caggttttat gtctgtaaac aaagaagtaa aaagggtaac gaccaacaca
     2521 ctgcaaaaaa tgaaaaacgc aggtgaaaag atttccatgc tgactgccta tgacttttca
     2581 tttgcaaaaa tcattgacag tgccggcata gatgtaatat tggtaggtga ttcagcttca
     2641 aatgtgatgg ccggacatga aaccaccctg cctatcacac ttgaccagat gatctatcat
     2701 gcttcttctg tgataagagg tgttgaaaga tgcctggtag tggttgacct tccctttggg
     2761 tcttatcaat ccaactctga tattgcattg gcctccgcta tacgtatcat gaaagaaacc
     2821 ggcgcccatt ctgtgaaaat ggaagggggt gaagaagtta tcgattccat ccgcaagatc
     2881 gtatcagcgg gtataccggt aatgggtcac cttgggctaa taccccagtc aatctacaag
     2941 tttgggacat acgctgtaag agccaagggg ggcacagaag ctgataaatt aaggagagac
     3001 gccaaattgt tggaggaagc aggttgcttt gccattgtac tggaaaaaat tcctgctgaa
     3061 ctggcaaaag aagtttcaga aagtttaact atacctacca ttggtattgg cgccggtggc
     3121 gattgcgacg gacaagtact ggtgatgcat gatatgctcg gaataaatac agaattcaaa
     3181 ccgaggtttt tgcgacagta cctgaatatt catgaactgg cagttaaagg agtacagcaa
     3241 tatatcagtg atgtgaaatc cggtgatttc ccaaatgacc aggaacaata ttaaaacccc
     3301 taattttata ttttactatt cattcaattt atgaaacaac aactattcct gttctttacg
     3361 gccatcacta ccctattttt atttcaatct tgtcaaaagg aaatttcatt tgacgatgac
     3421 aataacactg ttacaggcga tttcagggcc aaaatcgaag gctcacaatg ggtggccaat
     3481 aaaggagcag gagccagcag gatgcagggg ttaataaaca tatctggtta cagtacagac
     3541 aaaaagtata taaccattac cctggcagat tccggtgtgc accggtacac actaagtgat
     3601 gccacgatga atgccgctgc atacatcgac agcacgctgc caaaccctat tgcatttaca
     3661 acgaaccagg gcacttatcc cacagaaagc ggtggcgaag taaatattac atctatcgat
     3721 acagccaata aaaaaatgag tggtacattt tcattcaaag tgttcagaat gatggatggt
     3781 cttggcagaa atattacaga aggaagtttt actaatttaa gctacaccac aacattgccg
     3841 cctgccaatg ccactgatac ttttaaagtg aaaatttccg gtacacaatg gaatccaccc
     3901 accatatttg cggtaaaagc ccccgctgtt cctccattac ctgctacaat agctgtttca
     3961 ggcactgatg caaccgcttc aaaagtagta ggcctaattt ttcctgcaga cattaccccg
     4021 ggatcctata ccctggattt ttttggaggc acctatatcg ggcagtataa tcctaatagc
     4081 aatcctcaaa attcacaggc ttccgtaagc ggtacgcttc agataatttc tcataacaca
     4141 tctactaaac ggatcagggg caatttcaat ttccggtcag aagagttact gaatccttta
     4201 gctttcacat tactaactga gggatacttt tctgtaacct atcactaagt tcagtagtag
     4261 gacaattatt ttaaaagctg tttaatcnnn nnnnnnnata aacagctttt tctatttaga
     4321 acaattacat gacaaactga ttatgagtac tcccctattt ttgcgcccaa aaccaacgta
     4381 tgagtattgc gaaaagggca ttttttgctg taatgcttct tacagtttcc catattattg
     4441 ttgcccagga cagcaccctg caattagata acgtcatcat caccgccaca ttatctgcac
     4501 agaaacaaaa agagagcgga agaaatatcg ttgccataaa aggtgaaagc ttttctaaac
     4561 tacctgttca ttctgttgac gatttacttc gttatctgcc ggggattgaa gtacagcaaa
     4621 gggggccaca gggatcacaa agtgacattg taatcagggg tggcactttt cagcaggtat
     4681 tggtgatcat tgacggtgta aaattaaacg atccgcttac aggccatttc agcatgtatg
     4741 taccaataca ttcgtctgaa atagaaagag tggaaatcct gaaaggcgct gcatctgcta
     4801 tctggggctc agaggctgta gggggtgtag taaatattat cacaaaaaca ttttccaata
     4861 gtaaaaacaa aaatcaaatt aaagccaaaa cggttgcagg agaatatgga ctgtttaatg
     4921 gcgatgcata tgtaagctat tccaaaaata aatctgcctt cgcagctggc attttgtcta
     4981 ataatgcaaa tggccagcca ttaagaggaa caaatggctt ctttcattta acaaccgcta
     5041 atatttcttt ctcacaggga ctggcaaaag actggaggtt aaacttcagg actgctgctg
     5101 atctcagaaa atttaatgcg caaaatttct atactacttt taacagcgat accgcggaag
     5161 aaaaagtaaa tacctggtgg aaccacctga atcttcaaaa gaaaacaaac cggggaaccc
     5221 tgaattttga tgctgcatat aaaaaactgc gggatcaata ctggttcagg ccagctagtg
     5281 ttcccaacga taacaggaca agcttattta cttcacagtt atattattcg gcagacatca
     5341 ataaaaaaca tggttatacc gggggcttac aagtgcatcg caaagaaatt cgttccaatg
     5401 acaggggcaa tcattcctta tggcatgctg cagcgtattc gatttttcgc cataaactta
     5461 aaaatggcat ttatctgaat gaaagtcttc gcctggactg ggacgagagt tatggctgga
     5521 tattatcacc tcagttgaat attgcatggg ctccttctaa gttaaccctc aggagttcag
     5581 ttggccgttc tttccgtgat gctgatttta ccgaacgata taataactat aataaagaac
     5641 tggtgtccag cggccgtatt ggcaaccctg acctggaagc tgaacaatcc tggaatgctg
     5701 aaattggcat tgattataat ttctccgccc gtttcagaat aagcggaact atcttttaca
     5761 gggaccatag taacctgatc gattgggcca atactcctta cgctgaaatg ccaaggaaat
     5821 cgaacctgag tccagcgggt aattatgcat tagccaaaaa tgttgaagaa gtaagaacga
     5881 gaggtatgga aatagatata atttacaatg aaaagcttgg tcaaaatgcc agccttttgg
     5941 ctaccgctgg tcttacctgg cttaaatcag taaatgatga ccctgtaccc tctttttata
     6001 tttcttcaca tgcaagattt atgaccaatt tctcgcttgc ctacaccatt cattctttct
     6061 ctgtttctgt aaatggtgta tataagcagc gggacgagca aaaatcttct gccattaatg
     6121 cagaaatttc tccctcttat tttgtgctga atacaaaggc cgcctattca accaaaaatg
     6181 gctggggaaa atgttttata caggccgaca acatttttaa taaaggatac agcgatcttt
     6241 taggcgctgt gatgcctggc cgatggttat caggcggaat tgagatagct ttgccctatt
     6301 aaaaaaacag tatggacttc ttaatcacat taaaaattga ctctaccaat aaaggcgtta
     6361 gcaccggaac acagtggatt aattcaaaag gtgaaataat cgattcattc tctcctgttg
     6421 atgggaaaaa aattggttcc gtaaccgctg ctgataaaga aagctatgag gccgtgataa
     6481 aaaaagcaga agaagctttc aagacatgga ggcttgttcc tgctcctaaa agaggcgaaa
     6541 tagtaagaca gatcggagaa gcactccgac aatataaaga gccgttgggt aaactggttt
     6601 cctatgaaat gggcaaaagc ctgcaggaag ggctgggcga agtgcaggaa atgattgata
     6661 tctgcgactt cgctgtcggg ctttcaaggc aattacatgg tttgacaatg cacagtgaaa
     6721 ggcctggcca ccggatgtat gaacaatatc accctttggg gattgtaggg attatctctg
     6781 cattcaactt tcctgttgca gtatggagtt ggaacagtat gctggcatgg gtatgtggtg
     6841 atgtctgcgt atggaagcct tctgagaaag ttccgctttg tggcatcgcc tgtcagcata
     6901 tcattacaga agttttcaaa aagaataatg taccggaagg agtttcagga ttaattgttg
     6961 gtggccgtga agtgggtgaa tggatggctg cagacagcag gataccgctg gtatctgcaa
     7021 caggctcaac aagaatggga aaagccgtag gtgctactgt tgctgcaaga ttaggcaggt
     7081 cattgcttga actgggtggt aataatgcta tcataatttc taaagaagcg gatttggata
     7141 ttgccattct cggagcatta ttcggcgccg tgggcactgc agggcagcgc tgtactacta
     7201 cccgcaggct gatcattcat gaagatgtat atgataccgt gaagaataaa ctcgtatcag
     7261 cttacaaaca attacgtatt ggcgatccgc tggaccagaa gaatcatgtt ggacccctga
     7321 tcgataaaga tgctgtaagg atgtacctgg attcgattga acaatgcaaa gcagaaggcg
     7381 gcaactttat tgttgaaggt ggtgttctct ctggtccggg ctatgaaagt ggttgttatg
     7441 ttaagccttg tattgccgnn nnnnnnnnnn nnnnnnnnnn nnnnnggatt ataaaattgt
     7501 acagcatgaa acatttgccc caattcttta tttgttgaaa tataaaacga tcgaagaagc
     7561 cattgccatt caaaatgaag taccacaagg actttcttct tcaatcatga cattgaatat
     7621 gcgggaagcc gaacagtttc tttcacatgc cggaagtgac tgtggtattg ctaatgtgaa
     7681 cattggaacc agcggtgccg agattggtgg agcttttggc ggggaaaaag aaaccggtgg
     7741 tggccgtgaa agcggaagtg atgcatggaa agcttatatg cgaaggcaaa ctaatacaat
     7801 caactacagc acatcgctgc cattagcaca gggaattaag tttgatgtat aaataaatac
     7861 taaaaggttg acctaaatca tgaacaggca accttttatc tataccaccc gtcaaaaaaa
     7921 tcctaaaaag ccccttattc agggcttttt gtatgcaaaa tgtatgaacg gtaatcagta
     7981 tttttacgcc tacaccaatt ttacagttaa aataaaccaa atgaacagaa cagtaaaacg
     8041 tacctctatc ctgttttttt caggccttat ctctacggca gctcttgcac aaaaagacga
     8101 gatcccaaaa ggctggcaca tgatggacaa agcggcatcc ggctatcatg gtatcagcat
     8161 agataaagct tacgagtttg tcaactcaaa gaaacttaaa agtaatacag ttattgtagc
     8221 tgttatcgat tcaggcattg ataccctgca cgaagacctt aaaccggttt tgtggaaaaa
     8281 cgccaaagag attccgggaa acggaattga tgatgataag aacgggtatg tggatgatgt
     8341 ttatggctgg aactttttag gaggccgtga tggaaaaaat gtagagtccg actcatacga
     8401 agcagcaagg gtctttcata aattcaaatc caaatacagt ggaaagacca ttgataaatc
     8461 agcgctcagc aaagaagaac tatttgaata cgaaatgtgg aagagatcag aaggtgaaat
     8521 agccggtggt gattctaaaa gtaatgccct ggaactcatt ttcctgaaaa gagcttatac
     8581 caactgtgta aaagcagaca gtatcctgcg caaagcgatg ggtaaggatg tttacacagg
     8641 aaaagaactg ggtgaattca gttcagatga tactgatgtt aaaaaatcca agaacacatt
     8701 atatggacta atgtcgggta atgatgccct cgaatcaact aataaagatt tcctggaagg
     8761 attcggtgat tatgtttccg gggaggaaaa gaaagcacag gcagcagaaa cagctcctac
     8821 cccttatagg gctgatattg ttaaagacaa ttatgatgac atcaacgaca aattttatgg
     8881 taacaataat gtaatggtga gtaataaatc cgcattacac ggcacccatg tttccgggat
     8941 catcgccgca gcccgcaaga acggtgtagg tatggatggt gttgcagaca atgtaaagat
     9001 catgactctt cgggccgttc ctgatggtga tgagcatgat aaagacattg cactggctat
     9061 ccgttatgca gtagataacg gagcacaggt gatcaatatg agttttggta aaggtttttc
     9121 acccgaaaag aaatgggtgg atgatgcagt gaaatatgct gaaagtaagg gtgtgctgtt
     9181 ggttcatgct gcaggcaatg atgccaagaa ccttgatact actttcaact accctactcc
     9241 acagttattg gatggatcta ggccttccaa ttggttaacc gttggtgcca gtggtgatcc
     9301 taaagctggt ggtttaacag caagcttttc caactatggt aaaaatgaag tggatgtatt
     9361 tgctcccggt gtaaaaatct attctacagt accaggtggc aacacttacc aaaatctcca
     9421 gggtacaagt atggcctcgc cagtggtggc aggtttggca gctttcattt tacaatatta
     9481 cccaaccctt agcccggcac aggtgaaaat ggtgattgaa aaatctgctc aaagtccggg
     9541 gacaacagta aaaaatccgg gcaatgatga aatggtagat ttgagcgaac taagcagggc
     9601 tggcggaatt gtgaatgcct acgaagcagt gaaattagcc gcttcactaa aaggcgaaag
     9661 aaaacctgca gtagtaaaac cggcaactac tgtcaaaccc aaagcaagaa attaatcaaa
     9721 aagccccttc taaggggctt tttgattcac cggattaatc ggaaaataaa aagagttact
     9781 ttagtaaccc gtaattatta aaatacctgt tatgagaaat tttctttttg gaactgtttt
     9841 tttctcgact ttattaatag ctggttgtgg cagtagtgat gaagagtctg atgaaacgac
     9901 taatacaaca actacaacag ccccaccttc agctgttaca gcggatacaa accaaaataa
     9961 aattgcatta ccagcaacta atacagttac agcacctgtt ctgaataatc agccggttgt
    10021 taccagttct gcaggaataa accctgaaca tggtaaaccc ggccatcgtt gcgatatagc
    10081 cgtaggagca ccattggata gtaaaccaac tcaacctgtg gctaaccctt ctgtcagtat
    10141 acctgcaaac ggtcaaaaat cggcacctgt gatcagtgct ccgactataa caactcctgt
    10201 agttacaccc accgttactc ccacaacaac acccgttact ggtgccggcc ttaatccaga
    10261 acatggcaag cccgggcatc gttgcgatat tgctgtcggc gctccattaa atagtgcacc
    10321 tgcgactaaa acaacccaac cagccgcaac aatcactccc agtatttctc ctgctccggc
    10381 cgttcagcct gtagcaacac ccgtaactcc ttctgtaaat actgatggaa cagcgttgaa
    10441 ccccgaacat ggtaaacctg gtcaccgctg tgatattgca gtcggcgcac cattaaacag
    10501 caaaccaaaa caataaataa ttaaatgggt aaacctgatt tgtcaagaat acctgagtgg
    10561 taccatggat atattaataa agtaaaggaa gacgatttaa cggcagcctt taatattcag
    10621 acaccccctt ttttaaagtt ccttgaagaa ttacctgaag caaaaagaga ttatcgttac
    10681 gctgatggta aatggaccgt taaagagatg ctgcagcaca taattgatgc tgaaagaatt
    10741 tttgcatacc gggctctccg gtttgcccgc aaggatagaa cgccattacc aggatttgac
    10801 gaaaatgaat atgccaaaaa cgccttagta tcaaacagag actgggataa catggtagaa
    10861 gaattcaggg tattgcgaaa atcaactgaa ttaatgtttg catcttttag taacgaagaa
    10921 ttagaactat caggaactgc aagcgagaaa ccggtatacg tactgggaat tggttttatc
    10981 attattgggc atgttgccca tcaccgggac attataaagg aacgttatct ctgagttttc
    11041 tatactgcaa agctggtacc acagccacaa gtagtgctcg cattagggtt cttaaaagta
    11101 aatcccctgt tatttaaacc atcctgccaa tccacctcca taccgtatag ataaagttca
    11161 tgcgatttgt ccataatgca ggatatgccg tctatttcaa aagtattatc attttccttt
    11221 ggctgatcaa aacccagcac ataagtcata ccagaacaac cgccaccttt tacgcctatt
    11281 ctaagtttct gggtggcatc aaaaccgggc tcattcatca gttttcttat ttcagcaaca
    11341 gctccggctg ttaatctaac aggtactata gttgttgttt ccattataca aaaataaaaa
    11401 ttctttaacc agtataacaa caaacccttt tcgaagaagg gaaatttaac aggtcattcg
    11461 ctgataatta tcaatcaaaa gattaaacaa tcggcctaaa tttgtcgtat tactttcata
    11521 aaccaacata catggatctg accgttgtta gcattatcct tgcctcttta gctgtcataa
    11581 tttctgtaat tgctcttttt agaaaatctg atacaccgcc actggaaccc gcaggtgaaa
    11641 aatttgacag tacttcattg cgtttacagg cttacgaaag acttgtatta ctggccgaaa
    11701 gaatttcctt gcctaacctg atcagcagac ttaatcagcc gggtattccc gctacggata
    11761 tgaaaattat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaaaatatt atgatcatca
    11881 accaggtctc agcaactatg ccggctgatg catctgcctc agacctgaat agaaaaattc
    11941 tggaggtaac aatgtcgcaa cccaatggct cattacatga aatggtgttg caggcattga
    12001 actttgaagc aaaaaaactg atgaactaaa tcgtagagct atggcagacg ataataagaa
    12061 atttaccgac tcaggaattg aaattaaacc gctttacacc agcagtgatt ggagcaatcc
    12121 ggaaaatgaa ttgcccggta attacccttt taccaggggt gtacaggccg atatgtaccg
    12181 tggtaaattg tggacgatgc ggcagtatgc cggcttctct accgccgaag aaagcaataa
    12241 acggtaccac tatctattat cgcagggtgt taatggatta agtgtagctt ttgaccttcc
    12301 cacacaaatc gggtacgaca gtgatcatca cctagctgaa ggagaagtgg gtaaagtagg
    12361 tgttgctatt gacagtattg aagatatgca gatactcttc aacggaataa aactggaaga
    12421 agtaagtact tccatgacca ttaatgccac cggttttatc ctgctctcat tatatgctgc
    12481 actggcaaaa cagcagggtg ctgatcttaa aaaaatatca ggaacaattc aaaacgatat
    12541 cttaaaagaa tacgcagcaa gaggcaccta tatttatccg ccaaaaccat ccatgcgtat
    12601 catcacagat atctttgaat ggtgcagcaa agaagtacca aagtggaaca ccatttctat
    12661 ttcgggttat catatcaggg aagccggaag tactgctgta caggaaatag cttttaccct
    12721 cagcaatggt aaagcatatg tgcaggctgc cttaaataaa ggtcttgata ttaatgtttt
    12781 tggcaaacgg ctgtccttct ttttcaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnntgaggaa gtggccaaat tcagggccgc cagaagaatg tgggctaaaa taatgaaaga
    12901 gctcggcgcc acagatccga aagcgatgat gctgcggttt catacacaga caggtggaag
    12961 tacattaacc gcacagcaac ccctgaataa tatttcaagg gttactattc agaccctcgc
    13021 agcagtattg ggtggtacac aatcattaca cactaatggc tatgatgaag cactaagcct
    13081 gcctactgaa gaagctgcca ggatagcctt acgcactcaa caaattgttg catttgaaag
    13141 tggtgttacc gaaacggtag accccctggc tggttcctat tttgtagagt ctttgaccca
    13201 acaggtagaa gaggctgnnn nnnnnnnaaa ctaattgaaa aaatcgatgc tatgggcggt
    13261 agcgtaacgg ctattgaaca aggatttata caggatgaga ttgcccgaag tgcctacgaa
    13321 taccagcgaa atattgaaga cagcaataaa ataattgttg gtgtaaataa attccaggtc
    13381 gccagcgagg atcatatacc catactgaaa gtggatgata gtatcaggac tttacaatca
    13441 gccaaacttg caaaactgcg cagcaacaga gataatgcca aggttgatca atgcctgcag
    13501 gaattgaatg ataaagctgt aagtggggac aatttgatgc ccgcagtact ggaagccgta
    13561 gagagtaagt gtactcttgg tgaaattgct gataccctaa gagaggtatt tggtgaatat
    13621 aaatagcccc aaactggcaa aatcctgcaa aaaaacccct aaaagtggta tttgccgggg
    13681 ttggcagatg tcctatcttt tcattccaaa accatctgtc atgaaaccat taaccttaac
    13741 cagggcatta gtgtgcctta ccttccttgt tttaagtact gaaagttttt cccaggtcaa
    13801 aatcggggga aacccaaaag aagatcccca cccttcggct gtattggagc tgcagggaac
    13861 tgataaaggt ctcttgttgc ccaggctgtc acaacagcaa atgcactcta ttaaacaacc
    13921 tgccaacgca ttggtagttt acaatacaga tgcaaagaat gtttttattt atagtgagga
    13981 tcgtaaagaa tggatgcccc tgatgcagga agcacagcgt ttgggagaag actcttgtga
    14041 atgggaattt gatacttcta ctctccgtgt ttttcttgtc agaggatatc cgcttggaga
    14101 ttccgtttat tataatactg cccgccgcaa atttgttttc tcagataaaa taaatgttga
    14161 aggcagcaat acaccggcag atgtatttta tccgggtaag tacatattca agggaaatgc
    14221 ttcgggggtc tttcatgatt ctgcgtccct gaattttccc agtattacat taagcaattt
    14281 cctctttgca atcgataatg attcatttgc agtagctaat cctaaccttg cattttataa
    14341 cggtatgcgg atatcaacgc aaatgttgcc ttcggct
//
LOCUS       DAORLS010000042        14160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2302_length_14160_cov_2.203758, whole genome shotgun sequence.
ACCESSION   DAORLS010000042 DAORLS010000000
VERSION     DAORLS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_14160_cov_2.203758"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PK977_05045"
     CDS             complement(<1..994)
                     /locus_tag="PK977_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRF17509.1"
                     /translation="MSKKVVLGFSGGLDTTYCVKYLSEDKGYEVHSIIVNTGGFSQEE
                     LSKIEAHAYKLGVKTHTTVDAVKGYYERIIKYLIFGNCLKNNTYPLSVSAERLSQALH
                     IAEHAQKLKADAVAHGSTGAGNDQVRFDMIFHIMIPGVEIITPIRDLKLSREEEIAYL
                     KSKGVKMNFEKAMYSINKGLWGTSVGGKETLSSKGMLPEEAWPTQVTKTGTEEIKLGF
                     DKGELKSVNDQYFTHPADAIQYLQTIAGPYGVGRDIHVGDTIIGIKGRVGFEAAAPML
                     ILKAHHALEKHVLTKWQLQWKDTLAQFYGNWMHEGQILDPVMRDIEAFLQNSQQN"
     gene            complement(1009..1722)
                     /locus_tag="PK977_05050"
     CDS             complement(1009..1722)
                     /locus_tag="PK977_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF17510.1"
                     /translation="MENQNIIIRVATVSDKVYSRTITDEMEASAAARGTGIAKRSPEY
                     IEKKIDEGKAVIAITDQNEWVGFCYIETWSHGEYVANSGLIVAPSFRKSGVAKQIKKK
                     IFDLSRQKYPEAKIFGLTTGLAVMKINSELGYEPVTYSELTQDEAFWAGCKSCVNYEI
                     LMSKERKNCMCTAMLYDPKDHYEPEETTKYFEENKKGFERLLKFKQWKLLKPFMRKDR
                     ESNGGAGKPKSLLHYFFHL"
     gene            complement(1820..1942)
                     /locus_tag="PK977_05055"
     CDS             complement(1820..1942)
                     /locus_tag="PK977_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17511.1"
                     /translation="MTNILTHTKQFAFHAPITSGVYSVGFDVFTFSRPRRRPVI"
     gene            2034..>2434
                     /locus_tag="PK977_05060"
     CDS             2034..>2434
                     /locus_tag="PK977_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17512.1"
                     /translation="MEAATPFENYQPLDEPALVKEKRPSRFRRFSRNFIIIFILLLAG
                     LIYWKYFFTYSEGNRTGLLQKFSYKGTIFKTYEGELILSSIRSQANVALASEKFFFSV
                     IDEETARKLENLQGHFITVHYKMKNGKLPWRG"
     assembly_gap    2435..2517
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2518..3182)
                     /locus_tag="PK977_05065"
     CDS             complement(<2518..3182)
                     /locus_tag="PK977_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRF17513.1"
                     /translation="MGEVYLIPSLLYEDGLNTIPAYVLDAIKNCQVFFAENERTTRRY
                     FKQLWKEMVIDDYEWYTIDDSSGGKFRSLLKEGKNIGIISEAGCPGVADPGQSLIAVA
                     QEMNATIRPLVGPSSILLALMASGMNGQQFQFIGYLPIDNLQRVKAIKELEAQSSRKN
                     CTQIFIETPYRNNQLMDALIKNCKSSTRLCIAKNLTGKDEYIRTKTVSEWKSALPDLH
                     KQP"
     gene            complement(3186..>3470)
                     /locus_tag="PK977_05070"
     CDS             complement(3186..>3470)
                     /locus_tag="PK977_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRF17514.1"
                     /translation="YLLALIILSLVPVVGWITPVIALFMECYYFGFSMLDYNFARLGF
                     SPQQSISFTGRHKGLAIGNGMLFYLMHIVLILAPAYAIIAATLSVQKIKE"
     assembly_gap    3471..3506
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3507..3929)
                     /locus_tag="PK977_05075"
     CDS             complement(<3507..3929)
                     /locus_tag="PK977_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRF17515.1"
                     /translation="MLKEIVIAFQGWDQARLFIQQHRLFKWILVPGFIYTVLFIVGMF
                     FFWQSSDSVISWVSNQLGIESWLQKERSEWLSFLFVMTGMMLRLILVLFYFSFFKYLI
                     LIIGSPAFAYLSEKTEAIIEGREHHFNWPELKKDCIRNS"
     gene            4012..4617
                     /locus_tag="PK977_05080"
     CDS             4012..4617
                     /locus_tag="PK977_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRF17516.1"
                     /translation="MKRLTKEFYRRSDVLKVAKELLGKILVTNWNGVITSGRIVEVEA
                     YAGINDMASHARHGRRTARNEIMYAAGGVAYVYLCYGIHHLFNVVTHGKDIPHAILIR
                     AIEPVKGIELMLERTGKKRPDHTLTKGPGNVSKALGIYTEHSGHTLSGRQLFIADDGF
                     TFSNNEIGSSPRIGVDYAGTDAHLPYRFYVKANPYVSGKPK"
     gene            4646..6118
                     /locus_tag="PK977_05085"
     CDS             4646..6118
                     /locus_tag="PK977_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRF17517.1"
                     /translation="MTKHQLKYISLLAGVIIALILYFINPFNVPEHSARILAVGGLMV
                     TWWVTEALPMPVVALLPLILFPLLEISSIKDTSKLYGNEVIFLFMGGFMLGLAIEKWN
                     LHRRIALSIVNVTGTSSNRIVLGFILATGLLSMWLSNTATTMMMFPIAMSVILVMKDN
                     GDENGNYANFNLCLMLAIAYASNFGGISTIIGTPPNVQFVNYFEKKYGNIDFTDWMLI
                     CTPLALLLMLALYFVTTRILFPSNIKSSEAAHEHIHNELKKLGKLSVSEKRVLFIFIS
                     TALLWIFKDIINKSQALFKLDDNMIAIIGAVALFMCPAGKKQLLVQGENKEEEKPEML
                     LEWDDTKKMAWGILLLFGGGIALADAMEKAGLIQQLGAWMGQYAGSGLILVLTVVVIS
                     IFISEVMSNVAQVIVFAPVVCSLAEHVGMNPLILGLPMTLAASCASMLPMGTPPNAIA
                     FASGHIRLKHMIKAGLVMNLIAVVLITLFTWYVVPLIIKL"
     gene            complement(6176..7192)
                     /locus_tag="PK977_05090"
     CDS             complement(6176..7192)
                     /locus_tag="PK977_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF17518.1"
                     /translation="MKKIVSTLLVSVALVSVTNAQDPNFSQFFASPLTLNPALTGKFD
                     GTFRVAGNYRNQWPTINNAFVTKTASVDFGVLRGKIPDIDQMGVGVLGFTDRAGDGVL
                     VTNMAGLSLSYHKGLDENGYHSIGAGFQGGYVNKRLDVTKVVFEDELTPLGFVPGTTG
                     EIFTNKQINVSYVDVNAGVIYTGSTNGYNSFYVGASMYHINRPKESFQGGQFLLNART
                     TLQAGGRIPVGSYHNIHFSGIHSMQAKATNTVVGGAFSYNVNNSEENPTNVYLGAWYR
                     FNDAAIPYIGIEFSGIHIGATYDANTSSLKPATNTRGGMEISLIYIKKPVDPNAKKLN
                     CPRF"
     gene            complement(7439..8089)
                     /gene="upp"
                     /locus_tag="PK977_05095"
     CDS             complement(7439..8089)
                     /gene="upp"
                     /locus_tag="PK977_05095"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRF17519.1"
                     /translation="MIVNLSEQHSLVSNWVAELRDVNIQNDRMRFRRNLERIGEVAAY
                     EISKILPFEEKEIQTPLGIAEHKIMKEQPVLATILRAGLPLHQGLLNYFDKADNAFIS
                     AYRKHHKDGSFEISLEYISCPELENRVLIISDPMLATGASLVKTIQYLKDEGSPSAIH
                     IVAAIACTVGIEYVKREAPSVTIWCGDIDDELTAKGYIVPGLGDAGDLAFGTKIQM"
     gene            8247..9344
                     /locus_tag="PK977_05100"
     CDS             8247..9344
                     /locus_tag="PK977_05100"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRF17520.1"
                     /translation="MLYRTIGVMSGSSLDGLDIAFVEFQEQGGKWTYEIIEADCYSYT
                     SEWTDKLRNAINLPAREYQLLHTAYGHYIGQEINKFIEAHQLYYKVAVIASHGHTTFH
                     IPAQQMTAQLGDGAAIAAETQLPVVSDLRALDVAFGGQGAPIVPIGEKMLMNEYTYFL
                     NIGGIANISLNAEKYVAFDVCPANRVLNMLANEGGKAFDENGEMAASGMIDEGLLAKL
                     DSLEYYRQPYPKSLANDFGTDIVYPLIKSADLSIPDAIRTYSEHIALQICNALSSLNI
                     HQPVPGNKKLLVTGGGALNTFLVNRLAELLRVENIEVVVPDTKLVNYKEALIMAFIGV
                     LRWRQEYNVLASVTGASRNSIGGALWTGQEA"
     gene            9429..>10693
                     /locus_tag="PK977_05105"
     CDS             9429..>10693
                     /locus_tag="PK977_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF17521.1"
                     /translation="MGGKINMFGQEILTEKEEFCQLQQNFIPQYEAVFHDKMAPKTVV
                     VIPSLTLDPVILAKVDGIVHYEERLLCLLLLLRMPNTHVIYITSTPVDPIIIDYYLHL
                     LPGITGHHARQRLHFFSCYDASPQVSLTEKILARPRLIKRIKKAIPKDYLAHLACFNV
                     TDHERKLAVTLDLPVYGCNPDLLYWGTKSGSREIFRECGVDMPPGYENLQTEEDIIDA
                     LTDLHYKYPDLKKAVIKMNDGFSGEGNAVFRYEKLTAPDNIEQQIRELLPNELEIIAT
                     GLSYEMYIKKFEGMGGIVEAYVEGSHVASPSVQCRINPLGRIDVISTHDQVMGGLEKQ
                     IFLGGIFPADEEYRIEVGKLGQRISEALRNKGVLGRFGIDFLSVKQEGKWLHYAIEIN
                     LRKGGTTHPYIMLQFLTNGDYNAETGKFL"
     assembly_gap    10694..10713
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10714..10972
                     /locus_tag="PK977_05110"
     CDS             <10714..10972
                     /locus_tag="PK977_05110"
                     /inference="COORDINATES: protein motif:HMM:NF037725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HRF17522.1"
                     /translation="EEKCYLFTDNLHDERFKGLTPEDLIEIAVQNQLHYDNTREEGVM
                     FHLIGALSQYGKIGIVCIASTPERARYFYNKTIDVLKTECC"
     gene            11112..>14160
                     /locus_tag="PK977_05115"
     CDS             11112..>14160
                     /locus_tag="PK977_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF17523.1"
                     /translation="MRRLTKKLEAEILKAYYTYWDAYLKGDMKTFASFLDDSIITYGT
                     AGGEIFNSKKEAIKFYKATADQMTGKADFRKRKISAKAVGDTIVVNEQSDLYVLDDGS
                     WTFYGHSRITAIFEQKGNTWKLVHQHASFPDSRTEEGEQIATKKIKKENLELREAVRR
                     RTIELEEKNRELEIETALEKVRAVAMGMKERADMLKICKTISLQLAGLGVKDIRNVQT
                     AIFNVLAGTYMNYEYYAKHRKAFITETLYTNHKVARAFAAKMLKGKGEVSITHIRGKE
                     KVKEWLKYQKGTNVFIDTYLNAATSLSYYWFSLGPVALGISTYLPLSKEELQLFQRFL
                     KVFELAYRRYLDIEKAEAQAREAQIEAALERIRARALAMHSSDEFTDVARVMREQMGQ
                     LGQQEMETSAVHLYDEDAEYILSWRAFRLSSDLKSKINYGFFKIPKNSCKIAREFVQK
                     FKSKASEYTIEISGSIQTEWYKILFKLAPEVQAAMKKSGTTKEKRYYHFSKFSGGSLL
                     MVSSKEPSNNAIELQKRAAQVFDLAYSRFKDLQKAEAQAREAQVEAALERIRAASLAM
                     QDSSALGEIIYKLYGELTKLDAQLDRCFIMIVNPDNRGITWWMAGHEGLLAENGFFVP
                     MNQHPSHLMYLDYCKKRKKKWTYLFEGKEKRDWDRFGFTKTELAKLPEPIKAFMAAAK
                     KVHLSGSSDQFGSLVTGSFEPLPDEQQEIISRFAVVFNQAYIRFLDLQKAEVQAREAQ
                     IEAALEKVRSRSMAMQQPEELIEVAELLRREMGLLGVEELETSSIYIIDNEKKKAECW
                     YAIKDVRKENAQLIGDEMTLTLTDTWVGKEMWKFYQSANDRISILMKGERRREWINYC
                     AEHSKVLQGYYGDEIPERTYHLVKFYGGYMGAASPGTISKESWDLLKRAAVVFSLAYT
                     RFNDLQDAAARAREAQIELALERVRARSLAMHKSEELKEVIKVVLEQFVHLHINAEHA
                     GFYIDYKTHDDMHIWLADPNLEPFYAVIPYFDTPTWNSFLEAKA"
BASE COUNT         4151 a         2789 c         3054 g         4027 t
ORIGIN      
        1 cattttgctg actgttttgc aggaaagctt caatatctct cattaccgga tccaatatct
       61 ggccttcatg catccagtta ccgtaaaatt gtgctagggt atctttccat tgtaattgcc
      121 atttggtcag cacatgtttt tccaaagcat ggtgtgcttt taaaataagc atgggcgctg
      181 ctgcttcaaa accaacccgg cctttgatac caataattgt gtcgcccaca tgtatgtcac
      241 ggcctacgcc atatggtcct gcaatggttt gcagatattg aatggcatct gccggatgtg
      301 taaaatactg gtcattgact gatttcaatt cacctttatc aaatccaagt tttatttctt
      361 cagttcctgt ttttgttacc tgtgttggcc atgcttcttc cggtaacatg ccttttgagg
      421 aaagtgtttc tttacctccc acacttgttc cccaaagacc tttattgatg gagtacatag
      481 ctttttcaaa attcatcttc acgcctttac ttttcaggta ggctatttct tcttcacggc
      541 ttaatttgag gtctcttatc ggagtgatta tttcaacacc gggaatcata atatgaaata
      601 tcatatcaaa gcgaacctgg tcattgccgg caccagtgct tccatgagct actgcatctg
      661 cttttagttt ttgagcatgt tcagcaatat gcagtgcctg gcttaatctt tctgcagaaa
      721 cggaaagagg atacgtattg ttttttaaac agtttccaaa aatgaggtac ttgataatcc
      781 tttcatagta tccttttact gcatcgactg ttgtatgtgt ctttacaccc agtttgtaag
      841 catgggcttc gattttactc agttcttctt ggctaaaacc tcctgtatta acgattatac
      901 tatgcacttc atagccttta tcctcgctga ggtactttac acaataggtt gtatctaatc
      961 ccccgctaaa gcctaaaaca actttctttg acatttttta tgctgctttt ataaatgaaa
     1021 gaaataatgc agtaatgatt tgggtttacc ggctccgcca ttgctttccc tgtcttttcg
     1081 cataaatggc ttcagcaatt tccattgctt gaatttcagc aatcgttcaa atcctttttt
     1141 attttcttca aagtatttgg ttgtttcttc gggttcatag tgatccttag gatcgtagag
     1201 catagctgtg cacatacaat tttttctttc cttgctcatc agtatctcgt aattcacaca
     1261 gcttttacat ccggcccaaa aggcttcatc ttgtgtaagc tctgaatagg ttacaggctc
     1321 ataacccagt tcactattga tcttcattac cgcaagcccg gtagtaaggc caaatatttt
     1381 tgcctccggg tatttttgac gggagagatc aaatatcttt ttcttgattt gtttggcaac
     1441 cccgcttttt cgaaatgaag gcgctacaat caggccgctg ttggctacat attctccatg
     1501 gctccatgtt tcaatatagc aaaaaccaac ccactcattt tgatcggtaa tggcgatgac
     1561 agctttgcct tcatcaattt ttttttctat atattcaggt gaacgtttag caatgccggt
     1621 acctctggct gctgccgagg cttccatttc atcggtgatc gtcctggagt aaaccttgtc
     1681 agaaacggtg gctaccctga tgataatatt ttgattttcc acttttgttt atgcttgaga
     1741 tatagcggtt caccagtcct gaaaaatttg ttccggggtg atcttcactg acagggacca
     1801 tggtgagggg ttcgtcctgt cagataacag ggcgacgtcg tgggcgggaa aacgtaaaca
     1861 catcaaaccc aacagagtaa actccactgg taattggcgc atgaaacgca aattgcttag
     1921 tatgtgtcag aatattcgtc atgattatgt ttacaaacaa gttgtaaatt ggggacaaat
     1981 gtataataat aattggttta tagccataaa ttttttgtta taaaacacgt caaatggaag
     2041 ctgcaacccc ttttgaaaat tatcagcctt tagacgaacc agcgttagtg aaagaaaaac
     2101 gtccttccag gttcagacgg ttcagcagga acttcattat catcttcatc cttctactgg
     2161 ccgggttgat ctactggaaa tattttttca cttatagtga gggtaaccgc accggcttac
     2221 tacagaaatt ttcttacaaa ggaacaattt tcaaaaccta tgaaggtgag ttaatcttaa
     2281 gcagcatccg ctcacaggct aatgtggcat tggcttctga gaaattcttt ttttcagtta
     2341 tagatgaaga aacagccaga aaactggaaa atctccaggg acatttcata accgtacact
     2401 ataaaatgaa gaatggtaag ttgccatggc ggggnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag
     2521 ggttgcttat gaagatcagg taatgcggat ttccattcac taacagtttt tgttctgatg
     2581 tattcgtctt tccctgtcag gttttttgca atgcaaagcc tagtagaaga tttacagttt
     2641 ttaatcaatg catccatcaa ttgattattt cggtaggggg tttcaataaa tatttgtgta
     2701 caattcttac ggctggattg tgcttccagt tcttttatgg cctttactct ttgcagatta
     2761 tctattggga gatatccaat aaactgaaat tgctggccat tcataccact ggccattaat
     2821 gccaataaaa tagaacttgg tccaaccagt ggacgaatag tggcattcat ctcctgtgcc
     2881 acagcaatca atgactggcc cggatcagcc acacccggac aaccggcttc actgatgata
     2941 ccaatgttct ttccttcttt taataaactt ctgaatttac cgccagaact atcatcaatt
     3001 gtgtaccatt cgtaatcatc aattaccatt tccttccata attgcttaaa ataacgacgt
     3061 gtcgttcttt cattttctgc aaaaaatacc tggcaatttt tgatggcatc cagaacatat
     3121 gcaggaatgg tatttagccc gtcttcatac agcaaggaag gtatcaggta aacctctccc
     3181 atcaattatt cttttatttt ttgaacactt aaagtagcgg caataatggc ataagcagga
     3241 gccagtatca atacaatatg cattaggtaa aacagcatac cgttaccaat agccaatcct
     3301 ttgtgcctgc cagtaaatga tatgctttgc tgcggtgaaa aacccagcct cgcaaaattg
     3361 taatccagca tggaaaagcc aaagtaatag cattccatga acaatgctat taccggagtg
     3421 atccagccaa cgacgggtac caacgataaa atgataaggg ccagtaaata nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnngcta ttccggatac aatctttttt tagttccggc
     3541 cagttaaaat gatgctctct cccttcaatg atagcttccg tcttttcact caggtaagca
     3601 aaagcaggag agcctattat caggataagg tatttgaaaa aagagaaata aaaaagtacc
     3661 agtatcagcc gaagcatcat gcccgtcatg acaaacagaa aactgagcca ttcacttctt
     3721 tctttttgta accagctttc tatgcccagt tgattactta cccatgaaat gacactgtca
     3781 gatgattgcc agaaaaaaaa cattccgaca ataaaaagta ccgtataaat aaaacctggt
     3841 acaagtatcc atttgaataa gcgatgttgt tgtatgaata acctggcctg gtcccaaccc
     3901 tgaaatgcta tgactatttc tttcagcaaa gccttaattt gagcattaaa tatagtgaaa
     3961 ggggaatagt gtgtggtgaa tagatcagaa aaaagccaaa gcaggtgtaa aatgaagagg
     4021 ttaaccaaag aattttaccg gcgaagcgat gtgttgaagg tagcaaaaga gcttctggga
     4081 aagatactgg tgacgaattg gaacggagtt ataacatccg gcagaattgt tgaagtagag
     4141 gcttatgccg gtataaatga catggcttca catgcccggc atggcaggcg tactgcaaga
     4201 aatgagatca tgtatgcggc aggcggggtg gcatatgttt atttgtgtta tggcatccat
     4261 catctcttca atgtggtgac acatggtaag gatattccac acgccatatt gatcagagca
     4321 atagagccgg ttaaaggtat tgaactgatg ctggagagaa caggtaagaa aaggccagat
     4381 catacgctga caaaaggccc ggggaatgta tcaaaggcac taggtattta taccgaacat
     4441 agcggccata ctttatccgg aaggcaacta tttattgcag atgatggatt cacattttca
     4501 aataatgaaa tcggctcatc tccccgtatc ggtgtagact atgcaggtac tgatgcccat
     4561 ttgccgtacc ggttttatgt aaaagctaat ccctatgtga gcggcaagcc taaataggtt
     4621 gtaatttccc atcaaatctg gctgcttgac caaacatcaa ctgaaatata tttctcttct
     4681 ggcaggagta attattgctc taatacttta ttttattaat ccatttaatg tgccggagca
     4741 ttctgcaaga atacttgcag taggcgggtt gatggtaacc tggtgggtta ccgaagcctt
     4801 gccgatgccg gttgtggcct tattaccatt gattcttttt ccactgcttg aaataagctc
     4861 tatcaaagac acatcaaaac tttatggcaa cgaagtaatc tttcttttca tgggaggatt
     4921 tatgctgggg ctggctattg aaaaatggaa ccttcaccgg cggatagctt tgtccatagt
     4981 caatgtaaca ggaacgagca gtaaccggat cgttctgggg ttcattttgg caacaggact
     5041 gctaagtatg tggttaagta atacagctac caccatgatg atgtttccca ttgcaatgtc
     5101 cgtaatactg gtgatgaagg acaatggtga tgaaaatggt aattatgcca atttcaatct
     5161 ttgcctgatg ctggcaattg cctatgcatc aaattttgga ggtatttcta ccataatcgg
     5221 aacgcctcca aatgttcagt ttgtcaatta ttttgagaaa aaatatggca atatcgattt
     5281 tactgattgg atgctgatct gtaccccatt ggcattgttg ctgatgctgg ctttatattt
     5341 tgttacaacc cgtatcttat ttcccagtaa tattaaaagt agtgaagcag cccatgagca
     5401 tatacacaat gaattaaaaa aactgggaaa actatctgta tcagaaaaaa gagtgctttt
     5461 tatttttatc agtactgctt tactatggat attcaaagac atcattaaca aaagccaggc
     5521 attgttcaaa ctggacgata atatgattgc cattattggg gcagtagctt tatttatgtg
     5581 tcccgccgga aaaaaacagc tcctggtgca gggtgaaaac aaggaagaag aaaaacctga
     5641 aatgctcctg gagtgggatg acaccaaaaa gatggcttgg ggtattttgt tattgtttgg
     5701 tgggggtatt gctttagcag atgcgatgga aaaagctggc cttattcagc aattaggggc
     5761 atggatgggc cagtatgctg gcagcggact aatactggta ttgactgttg ttgttatttc
     5821 catctttatc agtgaagtga tgagtaatgt ggcgcaagtg attgtttttg caccggtagt
     5881 ttgttctctg gcagaacatg tggggatgaa tccgctgatt ttgggattgc caatgaccct
     5941 tgcggccagt tgtgccagta tgctgcctat gggaacgcca cccaatgcca ttgcttttgc
     6001 cagtggtcat atccggttaa aacatatgat taaagcaggc ctggtaatga accttattgc
     6061 agtggtgctg atcacattat ttacgtggta cgttgtacct ttaattataa agttgtaggc
     6121 aataaaaaat gcagatcaat aggtctgcat tttttattaa gtgtataatc ttttatcaga
     6181 atctagggca gttcagcttt ttagcatttg gatctacggg tttcttgatg tagataagtg
     6241 aaatttccat tccgcctctt gtattggttg ccggtttcag gctggatgta ttggcatcgt
     6301 aggttgcgcc aatatggata cctgaaaatt cgataccgat atagggaatg gctgcatcat
     6361 tgaagcgata ccaggcacca aggtaaacgt tagtaggatt ttcttcgctg ttatttacat
     6421 tataagagaa tgcaccgcct acgacagtat tggtggcttt tgcctgcata ctatgtattc
     6481 cggaaaagtg aatgttatga taggagccta ccggtatacg gccccccgcc tgtaaggttg
     6541 tcctggcatt taataagaac tggcctccct ggaagctttc tttcggcctg ttgatatgat
     6601 acatggaggc tccaacataa aaactattat atccattggt gcttccggta taaatgacac
     6661 ctgcatttac atcaacatag cttacattga tctgtttatt ggtaaaaatt tcaccggtgg
     6721 tgccgggtac aaagccaagc ggtgttaact catcttcaaa aaccacttta gtaacatcta
     6781 atcgcttatt tacatatcct ccctggaagc cggcaccaat tgaatgatac ccgttttcat
     6841 ctaacccttt atgataagag agagataaac cagccatatt cgtaacaagt acgccatcac
     6901 cggccctgtc ggtaaaaccg aggacaccaa cacccatctg atcaatatca ggaatctttc
     6961 ctcttaaaac gccaaagtca acggaagccg tttttgtaac aaaggcattg ttaatagtag
     7021 gccattggtt acggtaattc cctgctacac gaaatgttcc atcaaacttg cccgttaaag
     7081 ccgggttcaa agtcaacgga gaagcaaaaa attgcgaaaa gttaggatcc tgtgcgtttg
     7141 tcacacttac caaagccaca ctaaccaaaa gagtagaaac gatttttttc atcactgcca
     7201 ataaattttt tcacttttca gacggaggga tacagcagta aacgcttttt taaactaaag
     7261 attgccttat ccagttcctg tccggtacga aactgaaaaa tacgacaatt tacagacccg
     7321 caaaaagtaa ttgaccgggt tttgtagaat taattctatt caactgactt tgggctatcc
     7381 gccaaagaat cagttattta tacagaagta atcgttgtat ggaatcaata ttggtagttt
     7441 acatttggat tttggtgcca aaagctaggt caccggcgtc gccaagcccc ggtactatat
     7501 atcctttagc ggtcaattca tcatcaatat cgccacacca gatggtaact gaaggtgctt
     7561 ctctcttgac atactcaatt cctactgtgc aggcaatggc agccacaata tgaatggcag
     7621 aagggcttcc ttcatctttc aggtattgaa tggtttttac cagtgaggct ccggttgcca
     7681 gcatagggtc agaaatgatc aataccctgt tttccagttc ggggcaggaa atatattcaa
     7741 ggcttatttc aaaactgccg tccttatgat gctttcggta ggcagaaata aaggcattat
     7801 cagccttgtc aaaataatta agcaagccct ggtgcagggg cagtccggct ctcaatatcg
     7861 tggccaggac gggttgctcc ttcattattt tatgctctgc aatgcccagc ggggtttgaa
     7921 tttctttctc ttcaaaaggt aaaattttgc ttatttcata agcagctacc tcgccaatac
     7981 gttccaggtt gcggcgaaaa cgcatccggt cgttttgaat gtttacatcc cttaattctg
     8041 ctacccagtt actaaccaat gaatgctgct cacttaaatt aacgatcatc aattgaaaat
     8101 ttaatattgt tgattgaata ttgaggaaca atcaaaacct ttgcaccagc tcttgaaaag
     8161 cagcaaaacc taagtcctct ttctgcacaa aaatactatt tgcaggcggg cgggtaaaat
     8221 ctgaaatatt aaatctgaaa tatacaatgt tgtaccgtac tattggagta atgagcggca
     8281 gctcactgga tggactggat attgcttttg ttgaattcca ggaacagggc ggaaaatgga
     8341 cctacgaaat aattgaagct gattgttatt cttatacctc tgaatggaca gataaactaa
     8401 gaaatgccat caacctgcct gcaagggaat accagttatt gcatacagct tacggtcatt
     8461 atatcgggca ggaaataaat aagtttattg aggcccatca gctatattat aaagttgcgg
     8521 ttattgcttc tcacggacat actacttttc atataccagc acagcaaatg acagcacaac
     8581 tgggtgacgg ggcagccatt gctgcagaaa cacaattacc ggttgtatcg gacctcaggg
     8641 cattagatgt ggcatttggt ggccagggag cacctattgt accgataggt gaaaaaatgc
     8701 ttatgaatga gtatacttat tttctgaata tcggaggtat agctaatatt tccctgaatg
     8761 cagaaaaata tgttgctttt gatgtatgtc cggccaaccg ggtattgaat atgctggcca
     8821 atgaaggcgg aaaggctttt gatgaaaatg gtgaaatggc ggcatcaggt atgatagatg
     8881 aaggtttact ggcaaaatta gattcattgg agtactaccg gcagccctat cccaaatcac
     8941 tggctaatga ttttggaacg gacatagtct atcccctgat aaaatcggca gacttaagca
     9001 tacctgacgc catcaggaca tattccgagc atatagccct gcaaatctgt aatgcccttt
     9061 cgtctttaaa tatccatcaa cctgtaccag gcaacaagaa actgctggta accggcggtg
     9121 gtgcactcaa tacattcctt gtaaacaggc tggctgaatt acttagggta gagaatattg
     9181 aagtagtggt gcccgacacc aaactggtca attataaaga ggcactcatc atggccttca
     9241 tcggggtact tcgctggagg caggagtata atgtgctggc ctctgttacc ggtgccagcc
     9301 gtaacagtat tggcggggct ttatggacag ggcaggaagc ctgataaaaa tcactcgttt
     9361 atttagtttt tgtcattgcc gcctgaaggt ttacaaagta gcttacggat taactaacta
     9421 tttccctgat gggtggtaag atcaacatgt tcggccagga aatcctgact gaaaaggaag
     9481 agttctgcca gttgcagcaa aatttcattc cacaatacga agctgttttt catgataaaa
     9541 tggctccgaa gacggtggtc gttattccaa gccttactct cgacccggtc attttagcta
     9601 aagttgacgg gatagttcat tacgaagaga gattgctatg ccttttgctg ctgcttcgaa
     9661 tgcccaacac acatgtcatc tatatcacca gcactcctgt tgatcctata atcattgatt
     9721 attatctcca tcttctgccc ggaataaccg gtcaccatgc aaggcaacgg ttacattttt
     9781 tcagttgtta tgatgcatca ccgcaggttt cattgacgga aaaaattctg gcaaggcccc
     9841 ggttgatcaa gcgaatcaaa aaagcaatac cgaaggacta cctggcgcat cttgcatgct
     9901 ttaatgttac agatcatgaa agaaaattag ccgtcacact ggatttaccg gtttacggct
     9961 gtaatcctga tttgctctat tggggaacga aatcgggaag ccgggagata ttcagggaat
    10021 gcggggtaga tatgccgccg ggttacgaaa atctgcaaac ggaagaagat atcatagatg
    10081 cgttgacaga tctgcattac aagtatcccg atttaaaaaa agcggtgatt aaaatgaatg
    10141 atggcttcag tggggagggt aatgctgtat ttcggtatga gaaattgacg gcacccgata
    10201 atatcgagca gcaaataaga gaactgctgc ccaatgagct ggaaattatt gccacaggac
    10261 tcagctacga aatgtatata aagaagtttg aaggaatggg cggaattgta gaagcttatg
    10321 tggaaggaag ccatgtagcc tcaccatcag ttcaatgccg tatcaatccg ttaggaagga
    10381 ttgatgttat ttccactcat gaccaggtaa tgggcggttt ggaaaagcaa atctttctcg
    10441 gcggtatatt tccggctgat gaagaataca ggattgaggt tggcaaactg ggacaacgaa
    10501 tttccgaagc attgagaaat aaaggggtac tgggacggtt tggcattgac ttcctttccg
    10561 taaaacagga aggcaaatgg ctgcactacg ccattgagat caacctgcgt aaagggggga
    10621 ccacacatcc ctatatcatg ctgcagttcc ttactaacgg ggattataat gcggaaacgg
    10681 gtaaattcct tttnnnnnnn nnnnnnnnnn nnnagaagaa aaatgctatc tttttactga
    10741 taaccttcac gatgaaagat ttaaaggcct gacacctgaa gacctaattg aaattgccgt
    10801 gcaaaatcaa ctgcactatg ataataccag ggaagagggt gtgatgttcc accttattgg
    10861 tgccttgtcc cagtatggca aaattggaat tgtgtgtatt gccagcaccc cggaaagagc
    10921 ccggtatttc tacaacaaga ccatcgatgt gctgaaaact gaatgctgct gagcgtctct
    10981 tttttttcat tattcgcttt gtaataattg gttatattag gggaatactt tcctcccttt
    11041 ttaaactacg atttatttta agtcctgcta tcctgttacc tgggattttt tagaagataa
    11101 tttatactca gatgagacga ttgacgaaaa aactggaagc agaaatttta aaagcctact
    11161 atacctattg ggacgcctat ctcaaaggcg atatgaaaac ctttgcctct tttcttgatg
    11221 atagtattat tacctatggc acagcagggg gcgaaatttt taattcaaaa aaagaagcga
    11281 taaaatttta caaagcaact gccgaccaga tgacgggcaa ggccgatttc cgaaaaagga
    11341 aaatcagtgc aaaagcagtt ggcgatacaa ttgttgtgaa tgaacaatct gatttgtatg
    11401 tgctggatga cggttcatgg acattttatg gtcattcacg cattacagcc atttttgaac
    11461 aaaaagggaa tacctggaaa ttggttcatc agcatgcttc atttcctgat agcagaacag
    11521 aagaaggaga acagattgca acgaaaaaaa ttaaaaagga aaaccttgaa ctccgggaag
    11581 cagtcaggcg ccgcaccatt gaactggaag aaaaaaacag ggaactggaa attgaaactg
    11641 cattggaaaa agtccgtgca gtagcgatgg gcatgaagga aagggcggat atgctgaaaa
    11701 tctgcaaaac catttcatta cagcttgccg gtttgggggt aaaggatatc cggaatgtgc
    11761 agacagcgat atttaatgta cttgccggca cttatatgaa ctatgaatac tacgcaaagc
    11821 atagaaaagc atttattact gaaacactgt ataccaatca taaggtggcc cgggccttcg
    11881 cggctaaaat gctgaaaggg aaaggagagg tttctattac ccatatcagg ggtaaggaaa
    11941 aagtaaaaga atggctcaag tatcaaaaag ggaccaacgt tttcattgat acatatctta
    12001 atgctgcaac atcacttagt tattactggt tttccctggg cccggtggcc ctgggcatct
    12061 ccacttatct tcctttatct aaagaggaac ttcagttgtt tcagcgtttt ctcaaagtat
    12121 ttgaactggc gtaccgtcgc tatctggata ttgaaaaagc cgaagcccag gcaagagaag
    12181 ctcaaattga agcagcattg gaaaggatca gggccagggc gttggccatg cacagttctg
    12241 atgaatttac agatgttgca cgggtaatgc gggagcaaat gggacaattg ggacagcagg
    12301 aaatggaaac ctccgcagtg cacctgtatg atgaagatgc ggagtatatt ctttcctggc
    12361 gagcattccg cctgagcagt gacctgaaaa gcaagatcaa ttatggtttc tttaaaattc
    12421 ctaaaaactc ctgtaaaata gccagggaat tcgttcaaaa atttaaaagc aaagcctctg
    12481 aatataccat tgaaataagc ggttccatac aaacggagtg gtataagatt ctctttaagc
    12541 tggcgcctga ggtgcaggct gccatgaaaa aaagcggaac caccaaagag aaacggtatt
    12601 atcatttttc gaaattctca ggtggttcat tgctaatggt ttcttcgaag gagccctcaa
    12661 acaatgccat tgaattgcaa aagagggcag cacaggtttt cgatctggct tacagtagat
    12721 tcaaagactt gcaaaaagca gaagcgcagg caagggaagc acaggtagaa gcagccttgg
    12781 aaagaataag ggcggcttca ttggccatgc aggattcatc agcattgggc gaaatcatct
    12841 acaagttgta tggtgaactt acaaagctgg atgcacagct ggaccgctgt tttattatga
    12901 tcgtaaatcc tgacaacagg ggcatcacct ggtggatggc cggacacgaa gggctgctgg
    12961 cagagaatgg tttttttgta ccaatgaatc aacatccttc tcacctgatg tatctcgatt
    13021 actgtaagaa gcggaagaag aagtggacat acctgtttga aggaaaagaa aaaagagact
    13081 gggaccgctt tggtttcaca aaaacagaac tggcaaagct gcctgagccc atcaaagctt
    13141 ttatggctgc tgcaaagaag gtacaccttt ccggttcctc ggatcagttt ggttcactcg
    13201 ttaccggcag ttttgagcct ttgccggatg agcagcagga aatcatcagc cggtttgcag
    13261 ttgtatttaa tcaggcttat atccgttttc tggatttaca aaaagccgaa gtacaggcaa
    13321 gagaagcaca gattgaagca gcacttgaaa aagtacgttc ccgatccatg gctatgcagc
    13381 agccggaaga actgattgaa gtagcagagt tattgcgcag agaaatggga ttgctggggg
    13441 tggaagaatt ggaaaccagt tccatttaca ttatagacaa tgaaaagaaa aaagcagaat
    13501 gctggtatgc tatcaaagat gtacgaaagg aaaatgcaca actgatcggc gatgaaatga
    13561 cattgacact tactgataca tgggtaggta aagaaatgtg gaagttttat caatcagcaa
    13621 acgaccgtat ttccatattg atgaaaggtg agcgaagaag agaatggatc aactattgtg
    13681 cagaacattc aaaagtattg caaggctatt atggtgatga gataccggaa cgcacctatc
    13741 atttggtaaa attttatggc ggctatatgg gtgctgcctc acccggtact atttcaaaag
    13801 aaagttggga tttattgaaa agagccgccg tagttttttc acttgcctat acacgtttca
    13861 atgatttgca ggatgcagca gcaagagcac gggaagcaca aattgagttg gcattagaac
    13921 gggtgagagc cagaagtctg gccatgcata aaagtgagga actgaaagaa gtaataaaag
    13981 ttgtattaga acaatttgtg catctccaca tcaatgcaga gcatgccggc ttttatattg
    14041 attataaaac acatgatgac atgcacatct ggctggccga tcccaacctt gaaccattct
    14101 atgccgtcat tccctatttt gatacaccca catggaacag ttttttggaa gccaaagcaa
//
LOCUS       DAORLS010000043        14009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2321_length_14009_cov_2.178802, whole genome shotgun sequence.
ACCESSION   DAORLS010000043 DAORLS010000000
VERSION     DAORLS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14009
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_14009_cov_2.178802"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /gene="nrfD"
                     /locus_tag="PK977_05120"
     CDS             <1..1220
                     /gene="nrfD"
                     /locus_tag="PK977_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRF17524.1"
                     /translation="DITNFVWWVGIGHAGTLISAILLLFRQGWRTGVNRAAEAMTIFA
                     VMCAGQFPIWHMGRVWNAFFVLPYPNTRGPLWVNFNSPLLWDVFAISTYFTVSLLFWY
                     SGLIPDLATLRDRAKKKWAKMFYGVAAFGWTGSTKHWQRHEALSLVLAGLSTPLVLSV
                     HTIVSFDFATSVVPGWHTTIFPPYFVAGAIFSGFAMVQTLLVVTRKVLALEDYITIEH
                     IEVMNKVIVLTGSIVGIAYLTELFMAWYSAVEYENFAFFENRLNLNSPYGWSYWLMMG
                     CNVLSPQIFWFRKMRRNLFVTFFMSILVNIGMWFERFVIIVTSIYRDYLPSAWSTYYT
                     PTIWEVGFYLGTFGLFFTCYFLFSKFFPVIAIAEIKHILKRSGHTYKEKMDELEHEST
                     EEFGQKHGHDHGA"
     gene            1254..1850
                     /locus_tag="PK977_05125"
     CDS             1254..1850
                     /locus_tag="PK977_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRF17525.1"
                     /translation="MAVKKFVTGNFYDEAVLFPAVKKVRRAGYKLHDVYTPFPIHGLD
                     HAMGLRDTSLHTAGFIYGITGTATAVGFITWALTMDWPINFGGKPFFALPAWIPITFE
                     LTVLFAAVGMVLTFCYLCQLAPFVKKDHFNPRSTDDTFVMALECTSKTNDEEAMKFLQ
                     ELGAKEVQVQYKETGWWLGRYDRDGKRFERNKTEEAVH"
     gene            1869..2519
                     /locus_tag="PK977_05130"
     CDS             1869..2519
                     /locus_tag="PK977_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF17526.1"
                     /translation="MMKKFGIITILAVVAVAMVSCNKVRRSPGRAYMPDMAYSRAYET
                     YASTENLQQKGIHYTGQPVAGTLAKGDMNWVYPYKNDSAGYANSASVKNPLPKLEAGD
                     YMEAARLYLVNCGICHGEKLDGNGPLYKGGDGPFPNKPATLVGDAKYTAMTEGTMFHS
                     ITYGRNSMGGHASQLSTKQRWMIVHYIKEKQAASTPAPATGTGTAPAPAKDTTAKK"
     gene            2557..3792
                     /locus_tag="PK977_05135"
     CDS             2557..3792
                     /locus_tag="PK977_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HRF17527.1"
                     /translation="MSIKEKFIVPKRYNSIAIALMVIGVLAIIGLYVTHGAKDDAHKQ
                     ARFWASLLHNSVYFLLVVNASMFFICATTLAWGGWQMAFRRVPEAISAVVPVIGVISA
                     AILLAICFSDNHTIYHWTDAEHVKHDKILNFKKGFLNKGFFAFMTVFTVVAWSVLGWK
                     MRQLSRSLDEKPLATREEGKKYIWNNTVWAAIYIVVFALTVMSSIPWLWLMSIDAHWY
                     STMYSWYTFASSFVAGVALIALFVVYLKNSGYLEYTNEEHLHDLGKFMFAFSIFWTYL
                     WFSQFMLIWYANIPEETTYFKPRAQGIYSGIFWLMFIINFLAPLLLLMKRGAKRNYGT
                     ITFMALLIIFGHWLDFFQMVFPGPSPDHVPMLLYDFGVALGFVGLIMFVTGRALSKAP
                     LLAKNHPFVKESLIHHTQF"
     gene            3851..4912
                     /locus_tag="PK977_05140"
     CDS             3851..4912
                     /locus_tag="PK977_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRF17528.1"
                     /translation="MQTFFIVAILVLGFLITFQIAKASEYVAVLRGEERSRKQSNKIN
                     AFLLLAFLVVGLIGVYYCNERLKGKILGEPASDHGVLVDRMLYITLAITFIVFVITQI
                     ALFWFSYKYQESDKRKAFFYPHNNKLELIWTAIPAITLTILVGFGIFYWFKITGDAPK
                     DAMQVEVVGKQFGWEFRYPGKDGILGKKYFKNVSPENNNPAGQLWDDPANYDDVFVEQ
                     EMHLVVNKPVKLVIGAKDVIHDVGLAHFRMKMDAVPGTPTTMWFTPKYTTKQMKEKTG
                     NPDFVYEISCDQMCGKGHWSMRGTIIVETQEEFDAWLAGKKPQYLVANPDKDPAAKKD
                     TATAKAPAAASVTNIKTRN"
     gene            4949..>5608
                     /locus_tag="PK977_05145"
     CDS             4949..>5608
                     /locus_tag="PK977_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRF17529.1"
                     /translation="MSEVTNAQSGAVIAPAIHHDEHGVHHHKESFVSKYIFSMDHKTI
                     GKQFLITGIVWAIIGGLFSVLFRLQLGYPDQTFPILETVFGNWAKGGQIQPEFYYGLV
                     TIHGTVLVFFVLTAGLSGTFANLLIPLQIGARDMASPFLNMLSYWFFFLASVVMLSSI
                     FVHTGPASGGWTMYPPLSALRQAGEGQKIGTDLWLISMALFIVSSLLGGLNYISTILN
                     MR"
     assembly_gap    5609..5857
                     /estimated_length=249
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5858..6756
                     /locus_tag="PK977_05150"
     CDS             <5858..6756
                     /locus_tag="PK977_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRF17530.1"
                     /translation="PAMGMVSEILSVNSRKPIFGYMAMLGSLFAICILAFLVWAHHMF
                     VTGLNPFLGSVFVLLTLLIAVPSAIKVFNWLTTLWRGNIRFTPATLFAIGFVSLFISG
                     GLTGIFLGNSTIDIHLHDTMFVVAHFHIVMGVASFFGMFAGVYHWFPKMFGRYLNNTL
                     GYIHFWVTMIGAYLIFWPMHYQGLAGIPRRYLDKKGWVSFNQFGELDKMITVVSIIVF
                     AVQLMFVFNFFYSIFKGRKVTKQNPWGANTLEWTTPIKPGHGNWEGEIPEVHRWPYDY
                     GKDGREFIPQTEPIGENESKGH"
     gene            6830..7711
                     /gene="cyoE"
                     /locus_tag="PK977_05155"
     CDS             6830..7711
                     /gene="cyoE"
                     /locus_tag="PK977_05155"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRF17531.1"
                     /translation="MKKVKDYLLLIKPSLSIMVVFSSVMSFALTKGSESYVSFWKMIL
                     LLFAGGLLVTGSANAINQVVEKDTDAQMKRTAKRPIASGRLSVNEGWAFAIITGALGV
                     FILGNWFNWLSAGLAAFSLFLYAFIYTPLKKVNSIAVLMGAVPGALPCLIGWAAGDNN
                     LGIGGWVLFAFQFFWQFPHFWAIAWVAHKDYSSVGFKLLPADEGPTKFTALQTIIYSL
                     LLVPVSLAPFYTGMCSYNDLVGKISLALITLSNLFMIGRCVTLYRKMDVGSARQVMFG
                     SYMYLPVVMLAMLLSKS"
     gene            7897..8469
                     /locus_tag="PK977_05160"
     CDS             7897..8469
                     /locus_tag="PK977_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRF17532.1"
                     /translation="MDIVSTQQRKKLHPHKFTLWVALGSIIMMFAGLTSAYIVKSNQT
                     NWKPVSVPQIFWISTVVMVLSSLTLQMALRSFKQREMNKYRLLIGVTLLLGIAFVVCQ
                     WLGFQQLWRQQITFKGAGAGQFLYVIFGLHAIHVIGGVVVLLVMFIKAFLGRVKLYSA
                     VPVEVAATYWHFVDLLWIYLLVFFLFIGSK"
     gene            8514..9260
                     /locus_tag="PK977_05165"
     CDS             8514..9260
                     /locus_tag="PK977_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRF17533.1"
                     /translation="MATQATAHQTKWWSGGKSPFNLEYGKLMMWYFLMSDAFTFGAFL
                     ISYGTIRFSQNFWPDPNVVFNAFPGAGHANLPLAFVSVMTFILIISSVTMVLAVHAGH
                     KGDKQGVIKWLAWTIVGGIAFLGCQAWEWHHLITGEHAVLVDGKIEMWGQNMRHNPWG
                     PIAEGITHNGHHVRTPGPVAFGGYFFGITGFHGFHVFSGVVINIIMLIMTQKNVFQNR
                     GHYLMIEKAGLYWHFVDLVWVFVFLCFYLI"
     gene            9321..>9630
                     /locus_tag="PK977_05170"
     CDS             9321..>9630
                     /locus_tag="PK977_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRF17534.1"
                     /translation="MQHPAFEEVTYHHHADDATFKKKVRKTTILLSVITIIELAIGLM
                     IYNIHKGENPNELLILMFKGAVCILTLAKAYYIVSIFMHLGDEIRNMIMTIVVPLLLF
                     I"
     assembly_gap    9631..9643
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9881..>10483
                     /locus_tag="PK977_05175"
     CDS             9881..>10483
                     /locus_tag="PK977_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRF17535.1"
                     /translation="MNKTALYGILLALLLPLVAYFIVKQKSERDVKMPRHYLPDSIVT
                     VTKKGKRMEDTVWHKVKNLTLINQEGKTVNFDSLKGKIIIADFFFTRCPTICPAMTMN
                     MKRMAESIHNGKRVGDKTNKLVHFVSLSVDSERDSVPQLKKWADRFQVNPETWWLLTG
                     DKKEIYDFALNEVKLGLIDGEGVDTNFVHSDKFVLIDSNRH"
     assembly_gap    10484..10514
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10779..10816
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10817..11270
                     /locus_tag="PK977_05180"
     CDS             <10817..11270
                     /locus_tag="PK977_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRF17536.1"
                     /translation="HIFAAVNAVINSMVAVLLLAALVAVKSRKYQLHKQLMLAAMVLS
                     ILFLVSYICHHLLAGETRFGDVNHDGLVDEMEKAAVSGSRMIYYIILGTHIPLAGIIL
                     PFILFSAYRALTGEFDKHKKLVRITWPVWFYVAVTGVVVYVMISPYYH"
     gene            complement(11367..>11876)
                     /locus_tag="PK977_05185"
     CDS             complement(11367..>11876)
                     /locus_tag="PK977_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF17537.1"
                     /translation="IFTTAYNEYAIQAIRFSAFDYLLKPVDIEELQASVQRFLQSNDD
                     YRQQFELLKNIMHNIQAPSADEFRLALPTREGVHFLTPPEIIRCESLGNYTKFFVTHN
                     RSYLISRTLGEYDTLLTPYHFIRTHKSHLVNRKYISFIDHDGFVVLKDGSRVEVSRRR
                     KEDVMAALK"
     assembly_gap    11877..11993
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12120..>13209)
                     /locus_tag="PK977_05190"
     CDS             complement(12120..>13209)
                     /locus_tag="PK977_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF17538.1"
                     /translation="ISRLHFEDCVIYLKDERNDVLVQKAAYGPKSPVDLTIHQPIEIP
                     VGKGIVGTVAQTGKPELVNDTEKDSRYIVDDERRFSELAVPIIIDGMVAGVIDSEHSR
                     KNFFTARQLQILSAVSVLCANQLQSIKAEEEMQKASLELLENKRKAMESRLQSLRLQM
                     NPHFLFNALNSIQQMILANEEMVATRYLSRFSKLLRTILVHSDKEMVTLKDELEILNL
                     YVELEAIRFKDSFRYTIECNEEIDLDEVKVPTLLIQPFVENAIWHGLMHKEGQRELHI
                     LFSEKDDNLFCVIEDNGIGRDKARETGIVSGKGKSHTSKGIAVSLERLKTLHGENGHT
                     GSLHITDMKKETGEPAGTRVEICFPIQN"
     assembly_gap    13210..13347
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13348..>14009)
                     /locus_tag="PK977_05195"
     CDS             complement(<13348..>14009)
                     /locus_tag="PK977_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF17539.1"
                     /translation="QVTQKDGLMNSRVEALIRDNKNRMWIGNDVGIACFNIADSSLKY
                     FDETYGLSIQGFRIAAYCRTSEDEQIWGTERGLQYFFPDELYQYKAELKVNIDRIETR
                     NVMSNLTQSHTFDLKASDNYVSFYFSTIEYLTQLRTFYEYKLEGLDDKWIKVINQNFV
                     RYSSLSPGEYTFRVRASNDNKNWTESGNAITIRIATPYYQTWWFRILGALSAIAILLF
                     VI"
BASE COUNT         3524 a         2957 c         3158 g         3784 t
ORIGIN      
        1 gggatatcac caacttcgta tggtgggttg gtattggtca cgccggaacc ctgatttctg
       61 cgatcctgtt gttgttccgc cagggatgga gaaccggtgt aaaccgtgca gcagaagcga
      121 tgaccatctt tgcggtaatg tgtgcgggcc agttcccgat ctggcacatg ggccgtgtat
      181 ggaatgcctt ctttgtattg ccttacccca acaccagggg tcctttgtgg gtaaacttta
      241 actcaccact tctctgggac gtatttgcga tctctactta tttcaccgtt tctttattgt
      301 tctggtattc cggtttgatt cccgatctgg ctacactgcg tgacagggct aaaaagaaat
      361 gggcaaaaat gttttatggt gtggcggcat ttggctggac tggcagcacc aaacactggc
      421 agcgtcatga agccttgtcg ctggtactgg ccggtttaag tacaccgctg gtattgtcag
      481 tacacaccat cgtatcattc gacttcgcta cttcagtagt tcccggatgg cataccacca
      541 tcttcccgcc ttacttcgtg gcgggtgcca tcttctccgg ctttgccatg gtgcaaaccc
      601 tgttggtggt gacaagaaaa gtattggcat tagaagacta tatcactatt gagcatatcg
      661 aggtaatgaa caaggtaatt gtattgaccg gttctattgt gggtattgct tacctcaccg
      721 agttgttcat ggcctggtac tcagcggttg agtatgaaaa ctttgccttc tttgaaaaca
      781 ggctgaacct gaacagccct tatggctgga gctactggct catgatggga tgtaacgtat
      841 tgtctccgca gatcttctgg ttcaggaaaa tgagaaggaa cctgtttgtg accttcttca
      901 tgtcgatcct ggtaaatatc ggtatgtggt tcgaacgctt tgtgatcatc gttacttcaa
      961 tttaccgtga ttacctgccc agtgcatgga gtacttatta tacaccaacg atctgggagg
     1021 taggattcta tctcggaacc tttggtttat tctttacctg ttacttcctg ttctccaaat
     1081 tcttcccggt tattgcaatc gctgagatca aacatatatt gaagagaagt ggacatacat
     1141 acaaagagaa aatggatgaa ctggagcatg aatcaacaga agaattcggg cagaagcatg
     1201 gtcatgatca cggcgcttaa ttgaaaaaaa ctaacactct gaactaaaat aaaatggctg
     1261 taaaaaaatt tgtaacagga aatttctatg atgaagcagt gctgtttcct gccgtaaaga
     1321 aagtacgccg tgccggttat aagctgcacg atgtgtatac accgtttccc attcatggtc
     1381 tggaccatgc catgggcctt cgtgatacca gccttcatac ggccggtttt atctacggta
     1441 tcaccggtac ggctacagca gtgggcttta ttacctgggc tttaaccatg gactggccga
     1501 tcaactttgg cggtaagcct ttcttcgcat taccggcatg gattccgatc actttcgagt
     1561 tgacggtatt gttcgcagct gtgggcatgg tactgacatt ctgctatctc tgccagctgg
     1621 ctccttttgt aaagaaagac cactttaacc cccgttctac ggatgatacg tttgtaatgg
     1681 cattggaatg taccagcaaa accaatgacg aagaggctat gaagttttta caggaactgg
     1741 gcgctaaaga agtgcaggta cagtacaaag aaaccggctg gtggctgggc cgttacgaca
     1801 gggatggaaa aagatttgag agaaacaaaa cagaagaagc cgttcattaa tcattctata
     1861 atcaacagat gatgaaaaaa tttgggatca ttacaatact ggcggttgtt gcagtggcga
     1921 tggtaagctg caataaagta agacgcagcc ccggccgtgc ctatatgccg gatatggctt
     1981 acagccgggc atatgaaaca tatgcttcta ccgaaaacct gcagcaaaaa ggtatccatt
     2041 ataccgggca gccggtggcg ggcaccctgg caaaaggcga catgaactgg gtatatcctt
     2101 acaaaaatga ctctgccggc tatgctaatt cagccagtgt aaagaatcct ttacctaagc
     2161 tggaagccgg cgactatatg gaagcagccc gcctttacct ggtgaactgt ggtatttgcc
     2221 atggtgaaaa actggatggc aatggccctc tctataaagg tggcgatggt ccttttccga
     2281 acaagccggc aacattggta ggcgatgcta aatacacagc catgacagag ggaaccatgt
     2341 tccattctat aacctatgga cggaattcaa tgggcggcca tgcttcacag ctgagcacca
     2401 aacaacgctg gatgattgtg cactatatca aagaaaaaca ggcggcttca acaccggcgc
     2461 ctgcaacagg taccggcaca gcgcctgcgc ctgcaaagga taccacagca aagaagtaat
     2521 tgataattat ttgaaagcaa ctataaacga acactgatgt caataaagga aaaatttatc
     2581 gtaccgaaga gatacaacag tatagccatt gcactgatgg taatcggtgt gctggccatt
     2641 attggtttgt acgttacaca tggggcaaaa gatgatgcac ataagcaggc ccgtttctgg
     2701 gcatcgctcc tgcacaacag cgtttatttc ctgctggtgg taaatgcatc gatgttcttt
     2761 atctgtgcca ccaccctggc atggggcgga tggcaaatgg cattccgtcg tgtaccggaa
     2821 gctatctcag cggtggttcc tgttattggc gtcatcagtg ccgctattct gctggctatt
     2881 tgcttcagcg ataatcatac gatctatcac tggaccgatg cagaacatgt aaagcatgac
     2941 aagatcctca atttcaaaaa aggatttttg aataaaggtt tttttgcctt catgactgtt
     3001 ttcaccgtag tggcctggtc agtattgggc tggaagatga gacaactctc ccgcagcctc
     3061 gatgaaaagc cgctggctac aagagaagaa ggaaagaaat atatctggaa caatacagtt
     3121 tgggcagcca tttacattgt ggtatttgca ctgacggtga tgtcttctat tccctggtta
     3181 tggctcatga gcattgatgc acactggtac agcaccatgt acagctggta cacttttgcc
     3241 agctcatttg tggccggtgt ggcattgata gccctctttg tggtgtacct gaagaacagt
     3301 ggttacctgg aatacaccaa cgaagagcac ctgcatgacc tcggtaaatt catgtttgcc
     3361 ttctccatct tctggaccta cctctggttc tcccagttca tgctgatctg gtatgccaac
     3421 attccggaag aaacaacata tttcaaacca agggcacagg gtatttacag cggaattttc
     3481 tggctcatgt ttatcatcaa tttccttgct ccgttgttat tactgatgaa gagaggagcc
     3541 aaaaggaact acggcaccat tactttcatg gcgctgctga tcatttttgg tcactggctc
     3601 gatttcttcc agatggtttt cccgggccct tcaccagacc atgtgcctat gttgttgtat
     3661 gattttggtg tggctttagg ttttgtggga ttgatcatgt ttgtaaccgg cagggcatta
     3721 agcaaagcgc cgctgctggc caaaaaccat ccttttgtga aagaaagttt gatccatcat
     3781 acacagttct aagaacaaag tatataacct gtaataacat tttattattt agcatttaga
     3841 tctaaaactt atgcagacat ttttcatcgt tgccatttta gtgctcggct ttctgatcac
     3901 tttccagata gccaaggcca gcgaatatgt agcagttttg cgtggcgaag aaaggtcccg
     3961 taaacaaagc aacaagatca acgctttcct gttgctggcc ttcctggtag tggggctgat
     4021 tggtgtgtat tattgcaacg aaagattaaa aggcaagatt ctaggtgagc ctgcatctga
     4081 tcatggtgta ctggtagaca ggatgctcta catcaccctt gccatcacct tcattgtttt
     4141 tgttattaca cagattgctc ttttctggtt ctcttataaa taccaggaat ccgataagcg
     4201 taaagctttt ttctatccgc acaacaacaa actggagctg atctggactg ccattcctgc
     4261 cattaccctt accatccttg taggctttgg tattttctac tggttcaaga tcaccggtga
     4321 tgcacctaaa gatgccatgc aggtagaagt agtgggtaag cagttcggct gggaattccg
     4381 ttaccccgga aaggatggaa ttttaggtaa gaaatatttc aaaaacgtaa gcccggaaaa
     4441 taacaaccca gccggtcagt tgtgggatga tcctgccaac tatgatgatg ttttcgtaga
     4501 gcaggaaatg cacctggtgg taaataagcc ggtaaaactg gtgatcggtg ccaaagatgt
     4561 tattcatgat gtgggactgg cacatttccg tatgaagatg gatgctgtac ccggtacacc
     4621 cactaccatg tggttcacgc caaagtacac caccaagcaa atgaaagaaa agacaggcaa
     4681 ccctgatttt gtttacgaga tttcctgcga ccagatgtgc ggaaagggtc actggagtat
     4741 gagaggaaca attatagtgg aaacacagga agagtttgac gcatggctgg ctggtaaaaa
     4801 accacaatac ctggtggcaa atcccgacaa agaccctgct gcaaagaaag atactgcaac
     4861 agccaaagca cctgctgcag cttctgtaac caatatcaaa acaagaaact aaataaagca
     4921 aacagcatct gctgaaaaat aaaagactat gagtgaagta acgaatgcac agtccggagc
     4981 cgttatcgca cctgcgatac accacgatga acatggtgtt catcatcaca aggaatcatt
     5041 tgtttcgaag tacatcttca gcatggacca taaaaccatc ggtaaacagt ttttgattac
     5101 cggcatcgta tgggccatca tcggtggttt gttttcggta ctgttccgtt tgcagctcgg
     5161 ttatcctgac cagaccttcc cgatcctgga aacagttttc ggtaactggg caaaaggcgg
     5221 gcagattcag ccggagtttt actacgggct cgtaaccatt cacggtacgg tgctggtgtt
     5281 ctttgtattg acagccggat tgagcggtac attcgccaac ctgcttattc ccctgcaaat
     5341 tggtgcaagg gatatggctt ctccgttcct gaacatgctt tcctactggt tcttcttcct
     5401 cgccagtgtg gttatgttat cttccatatt tgtacataca ggtcctgctt caggcggatg
     5461 gaccatgtac ccgccgctta gtgctttgcg ccaggctggt gaaggtcaga agatcggtac
     5521 agacctgtgg ctcatcagca tggcattgtt tattgtatca tcattgctgg gtggtctgaa
     5581 ttatatctct accattttga acatgcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac ctgccatggg aatggtgtca
     5881 gagatactct cggtgaactc aagaaaaccc atcttcggct acatggccat gctgggctct
     5941 ttgttcgcta tctgtatcct cgccttcctg gtatgggcac accacatgtt tgtaaccggc
     6001 ttgaatcctt tcctcggttc cgtattcgtg ttgctaacac tgttgattgc ggtaccatcc
     6061 gccatcaaag tatttaactg gcttaccaca ttgtggagag gtaatatccg cttcacaccg
     6121 gctactttgt ttgcgatcgg ttttgtgagc ttgtttatct ccggtggttt gacaggtatc
     6181 ttcctcggta actcaacaat tgatattcac ctgcacgata ccatgtttgt agtggcacac
     6241 ttccacattg taatgggtgt ggcatctttc tttggcatgt ttgccggtgt gtatcactgg
     6301 ttcccgaaaa tgtttggccg ctacctgaac aatacattag gttatatcca tttctgggta
     6361 accatgatcg gtgcttacct gatcttctgg ccgatgcact accagggcct ggcaggtatt
     6421 ccccgccgtt acctcgataa gaaaggctgg gtttctttca accagtttgg tgaattggat
     6481 aaaatgatca ccgttgtttc tatcatcgta tttgctgttc agctgatgtt tgtgttcaac
     6541 ttcttctatt ctattttcaa aggaagaaaa gttaccaaac aaaacccatg gggcgccaat
     6601 acgctggagt ggacaacacc gatcaaaccg ggtcacggca actgggaagg agagattccg
     6661 gaagtacacc gctggccata cgattatggt aaagacggaa gagaattcat cccccagact
     6721 gaaccgatcg gggaaaatga gagcaagggt cattaattac cggtaaggta attagtgctg
     6781 gtgcgttaga gttagaaaga agtttttttg aaaatatagc gaaggcgaaa tgaagaaggt
     6841 caaagattat ttgttgctca taaaaccttc gctgagtatc atggtggtgt tcagcagtgt
     6901 gatgagtttt gcactgacga aaggatcgga gagttatgtg agtttctgga aaatgatact
     6961 gttgctcttt gcaggtgggt tgctggttac tggcagtgcc aatgccatca accaggtagt
     7021 agagaaagac acggatgcac agatgaaacg gacggcaaaa cgtcccattg cttccggccg
     7081 cttatcggta aatgaaggat gggcctttgc catcatcacc ggtgcgttgg gtgtatttat
     7141 cctcggtaac tggttcaact ggttatcagc agggctggct gcattcagtt tgtttctcta
     7201 tgcattcatc tatacgccgt tgaagaaagt aaattcaatt gcggtgttga tgggtgcggt
     7261 ccccggtgct ttgccctgtt tgataggctg ggcagccgga gataataacc tgggtattgg
     7321 cggctgggtc ttgtttgctt tccagttctt ttggcagttc ccccatttct gggctattgc
     7381 gtgggtggca cacaaagact attcatcagt aggctttaag ttattaccgg ctgatgaagg
     7441 gccaacaaag tttacggcat tgcaaacgat catttactcg ttgctcctgg tgcctgtttc
     7501 actggcgccg ttctatacgg gtatgtgcag ttacaatgat ctcgtaggca agatcagcct
     7561 ggcattgatc acattgtcca acctgtttat gatcggaaga tgtgtgacgt tgtataggaa
     7621 aatggatgtg gggtcagcaa ggcaagtgat gtttggcagt tatatgtatt tgccggtagt
     7681 aatgttagca atgctattga gtaagagtta gcagtaatgt tcttttaaat tgaaatgtat
     7741 gtcgtaagcc gccttctaca tccttcgaca agctcaggat gacaaaggct gactgttttc
     7801 tgtcatgttg agcttgtcga aacatagaca gaaaacggat ttcagaaaaa aagttgaaat
     7861 tcttcggcat agcaataaaa atatataaaa agagagatgg atattgtgag tacacaacaa
     7921 cgaaaaaaat tacatccgca caagtttacc ttgtgggtgg ctttaggcag tatcattatg
     7981 atgtttgccg gtctgaccag tgcttatatc gtaaaaagca accagaccaa ctggaaaccg
     8041 gtttctgttc cgcagatatt ctggatttcc accgtagtga tggtgctgag cagtttgaca
     8101 ctgcaaatgg cattacgttc ttttaaacaa agggagatga acaaataccg cttactgatc
     8161 ggtgtcaccc tgctgctggg cattgccttc gtggtttgcc agtggctggg tttccagcag
     8221 ctgtggcggc aacaaatcac tttcaaagga gccggcgcag gccagttcct ctatgtgatc
     8281 ttcggcttgc atgccattca tgtgataggc ggggtggtag tactgctggt aatgttcatc
     8341 aaagcctttt tgggaagagt aaaattgtac agcgctgttc cggtggaagt agcagcaacc
     8401 tactggcatt ttgtagacct cttgtggatc tacctgctgg tattctttct tttcatcgga
     8461 agcaaataga gaataaagta aataattgaa tcaagtaaaa tcagacaact gacatggcga
     8521 cacaagcaac agcacatcag accaagtggt ggagcggcgg taaaagcccc ttcaacctcg
     8581 agtatggtaa actgatgatg tggtacttcc tcatgagcga cgcatttacg ttcggcgctt
     8641 tcctgatatc ctacgggact atccgtttca gccagaactt ctggcccgat ccgaacgtag
     8701 tgttcaatgc gtttccggga gccggtcatg ctaacctgcc gctggccttt gtgagtgtaa
     8761 tgacctttat cctgatcatc agctcggtga ccatggtact ggccgtacat gcagggcata
     8821 aaggcgataa acaaggtgtg atcaaatggc tggcatggac catcgtgggc ggtattgcgt
     8881 tccttggttg ccaggcatgg gaatggcacc accttatcac cggcgaacat gcagtgctgg
     8941 ttgatggtaa aattgaaatg tggggacaga atatgcgtca caatccctgg ggcccgatag
     9001 cagaagggat cacacataat gggcatcatg taagaacacc aggacctgtt gccttcggcg
     9061 gctacttctt tggtattacc ggtttccatg gcttccacgt attttccggt gtggttatta
     9121 atatcatcat gctgatcatg acgcagaaga atgtattcca gaacaggggc cattacctga
     9181 tgatcgaaaa agctggtttg tactggcact ttgtagacct ggtatgggta ttcgtattcc
     9241 tttgcttcta tttgatatag tgactcaaat agttttaaag tttttgaatt tgatatttgt
     9301 attttgaaat ttaaataatt atgcaacatc cggcatttga agaagtaacc taccatcatc
     9361 acgcagatga tgccacattt aaaaagaagg taaggaagac aactatcctg ctttctgtga
     9421 tcacaataat agaactggct atcggtttga tgatctataa cattcataag ggagaaaacc
     9481 cgaatgaact gctcatcctg atgttcaagg gagcggtttg tatccttact ttagccaaag
     9541 cttattatat cgtatctatt ttcatgcacc tcggtgatga gatcaggaat atgatcatga
     9601 cgatcgttgt accgctgctg ctctttatct nnnnnnnnnn nnngctgcgt ttctttggga
     9661 tggaaattcg tacaagaacc tgcggaataa gtatgatcct catttcaaag aaaaaaccat
     9721 gcctgccaaa cataataaag cagacgacaa gacccatgag gtgaaaccgg cagagaaaag
     9781 cggaaaagag taacagatat actttagtat accaaaacca ttaaaccggc tgcctgttat
     9841 taacggacgg tttaatggtt tttaatttta aaaagacaaa gtgaataaaa cggccctata
     9901 cggaatactg ctggccctgc tgctgccgct ggtggcttac tttattgtaa agcaaaaaag
     9961 cgaacgggat gtaaagatgc cccggcatta tctgcccgac tccattgtga cggtaacgaa
    10021 aaaaggcaaa cggatggaag atactgtgtg gcacaaagta aagaacctta cacttatcaa
    10081 ccaggaagga aagaccgtaa acttcgacag cctgaaagga aagataatca tcgctgattt
    10141 cttttttacc cgctgcccta ctatctgccc cgctatgacg atgaacatga aacgaatggc
    10201 cgagtcaatt cataatggta agagagtggg cgataagacc aacaaactgg tccattttgt
    10261 ttcgctgagt gtggattcgg aaagggattc tgtaccacaa ctgaaaaaat gggccgaccg
    10321 tttccaggta aacccggaaa catggtggct gcttaccggt gataagaaag agatctatga
    10381 ctttgcactg aatgaagtga agctgggact gattgatggt gaaggagtgg acaccaattt
    10441 tgtacacagt gataagtttg tactgataga cagtaaccgc catnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnattctg tttcgctttc tcatttgtcg gccgacctgg tgatgctcac
    10561 gatggaaaaa gaccccaata aaaaaagttt ctttgccggc aaacttcagc ttattgcggt
    10621 tgttttcctg ctggctatat tcggagtagg gctattgctg ttcctgcttc gccaaaaaaa
    10681 taaatgatgc tgaacgctgc ctggaaaaag aacgacaaaa aagccaaatg gctcatcttt
    10741 accttttctg ctattgtatt tgcggcggta gtggccctnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnacac atttttgcgg ccgtcaatgc cgtgatcaat tctatggtgg
    10861 cagtgctgct gctggcagca ctggtagcgg tgaagagccg gaagtaccag ctgcataaac
    10921 aactgatgct cgcagcgatg gtgctctcca ttcttttttt agtgagctat atctgtcatc
    10981 acctgctggc cggtgaaaca aggtttggcg atgtgaacca tgatgggctg gtggatgaaa
    11041 tggagaaagc agccgtaagc ggcagccgga tgatctacta tattattctc ggtacccata
    11101 tccctttggc gggtatcatc cttccgttta ttttattttc tgcctaccgg gcattgaccg
    11161 gtgaatttga caaacacaaa aaactggtac gcattacctg gccggtatgg ttttatgtag
    11221 ccgtaacagg ggtggtggtg tatgtaatga tcagtccgta ctatcattaa gcggctgctt
    11281 acactaattt tttgtcatca catactttga aggttgggta gagttttatg catagtggat
    11341 aagaacaacg aatgcaggta gatattttat ttcaatgccg ccatcacatc ttcttttctc
    11401 cgccggctta cttctacacg gctgccatcc tttaaaacaa caaacccgtc atgatcaatg
    11461 aaggaaatat atttccggtt caccaggtgg cttttatgcg tccggataaa atgataggga
    11521 gtaagcaggg tatcatattc cccgagtgtt ctcgagatta ggtaagagcg gttatgggta
    11581 acaaagaatt tggtgtaatt ccccaatgat tcacagcgta tgatttccgg tggggtcagg
    11641 aagtgtacac cttcccgggt aggcaaggcc agcctgaact catcggccga tggcgcctga
    11701 atattgtgca tgatgttttt cagcaattca aactgctgcc ggtagtcatc attgctttgt
    11761 aaaaaacgct gaacggaagc ctgaagttct tcaatatcca ccggcttcag caagtaatca
    11821 aaagcgctga agcgtatcgc ctgtatggcg tactcattat acgcagtggt aaagatnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgcctg
    12001 ccgggaatcg ttgcagacca tcacctgccc cgcttcgggg atggtctttt cgatcatcag
    12061 ccgcaacgaa tcagtcgctc tttcttcatc atcgatcaat aatactttaa gcatataaat
    12121 cagttttgta tgggaaaaca tatttcaacc cgggtaccgg ccggctctcc cgtttctttt
    12181 ttcatatctg tgatatgcag tgaccctgta tggccgttct ctccgtgtaa tgtttttaac
    12241 cgttccagcg atacggctat tcctttactg gtatgacttt ttccctttcc tgaaacgatc
    12301 ccggtttccc gggccttatc ccggccaatt ccattgtctt caataacgca gaaaaggtta
    12361 tcatcttttt ccgaaaacag gatatgcagt tcccgctgac cttctttgtg catcagcccg
    12421 tgccagatag cattttccac aaaaggctgt atcagcagtg tcggcacctt cacttcatca
    12481 aggtcaatct cttcattaca ttcaatcgta tacctgaatg aatctttaaa acgaatggct
    12541 tccagttcta catacaggtt taatatttca agctcatcct tgagtgtaac catttcctta
    12601 tcactgtgca ccaggatggt acggagtaat ttggaaaacc gggacaagta ccgggtagcc
    12661 accatctctt cattcgccag tatcatttgt tgaatggagt taagcgcatt aaaaagaaaa
    12721 tgaggattca tttgcaggcg caggctttgc aaccggcttt ccatggcctt tcgtttattt
    12781 tccagcagtt cgagagaagc cttctgcatc tcttcttctg ctttgatact ctgaagctgg
    12841 ttggcacaaa gcactgatac agccgataat atctgtaact gccgggcagt gaaaaaattc
    12901 tttctggaat gctcgctgtc gatcacaccc gccaccatgc cgtcaataat gatcggcact
    12961 gccagttcgg aaaatcttct ttcatcatct actatataac ggctgtcctt ttccgtgtca
    13021 ttcaccagtt caggtttgcc tgtttgggcc acagtgccca ctattccctt gccgaccggg
    13081 atttcaattg gctggtgaat ggtcaggtca acaggactct tcggtccata agcagctttc
    13141 tgaaccagca catcatttct ttcatccttc aggtagatca cacaatcttc aaaatgcaaa
    13201 cggctgatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnngat aacaaataga aggatggcaa tggctgatag
    13381 ggcacccagt atcctgaacc accaggtttg ataatacggt gttgcaatcc ggatagtgat
    13441 tgcattgccc gattcggtcc agttcttatt atcgttgctg gcccttaccc tgaacgtata
    13501 ttcaccgggc gatagtgagc tgtaacgcac aaagttctgg ttgatcactt ttatccactt
    13561 atcatccagc ccctccagct tgtattcata aaaagtacga agctgggtta ggtattcaat
    13621 cgtactgaaa taaaaactaa cataattgtc cgaagccttc aggtcgaatg tatggctttg
    13681 ggtgaggttg ctcatcacat tccttgtttc gatccggtca atattcactt tcagctcggc
    13741 cttatattga tagagttcat ccgggaaaaa atactgcaga ccccgctccg ttccccagat
    13801 ctgttcatct tcggaagtgc ggcaatacgc agctatacga aagccctgga tactcaaacc
    13861 atacgtttca tcaaaatact taaggcttga gtcggctata ttaaaacagg cgatcccgac
    13921 atcattgccg atccacatcc tgttcttatt atcccttata agggcttcca ccctgctgtt
    13981 catcaggccg tctttctggg tcacctgtt
//
LOCUS       DAORLS010000044        13991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2331_length_13991_cov_2.056975, whole genome shotgun sequence.
ACCESSION   DAORLS010000044 DAORLS010000000
VERSION     DAORLS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13991
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_13991_cov_2.056975"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK977_05200"
     CDS             complement(<1..336)
                     /locus_tag="PK977_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRF17540.1"
                     /translation="MTERLLQYIWQFQYFNKTDLLTKNGEGVQIIFPGQHNTNQGPDF
                     SDAKIKIGNTIWAGTVELHLKTSDWQKHKHQLDKNYNNVILHVVWENDAMNSEIPIIE
                     LKGRVSKILL"
     gene            complement(401..964)
                     /locus_tag="PK977_05205"
     CDS             complement(401..964)
                     /locus_tag="PK977_05205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRF17541.1"
                     /translation="MSIILASQSPRRKQLLEWAEVPFEIVVADTDEDFPPDLHPDEIA
                     IHIARNKALAVQQRRNNDDIILAADTIVVLNEHIIGKPVHREEAITILMALSGEKHQV
                     ITGVVIRMGDKEISFADKTDVQFHDLTEKQIEFYVDKYKPYDKAGAYAIQEWIGVVGI
                     QSISGDFYNVMGLPVSRVVRELKKFNV"
     assembly_gap    1124..1238
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1239..>1616)
                     /locus_tag="PK977_05210"
     CDS             complement(<1239..>1616)
                     /locus_tag="PK977_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRF17542.1"
                     /translation="FMLSTTGLLFTILALPFLQKWYLILANAGCIVLLWVYSTNFKKS
                     LLVGNVVISLLTAWTILIIFFSKVNLSDAIGAGVANHYKFFRLAMLYAGFAFIISLIR
                     EAIKDMEDMPGDEKYGCRTMPIVW"
     assembly_gap    1617..1706
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1707..1946
                     /locus_tag="PK977_05215"
     CDS             <1707..1946
                     /locus_tag="PK977_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17543.1"
                     /translation="IDDVTGGRNQQGSQQQKSKMKTIIRRNILFIQRIVNAGLEQELR
                     QCNKDEIGQSHQFEESGYQSHGNSLRWKDTTELLS"
     assembly_gap    2011..2027
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2028..2505)
                     /locus_tag="PK977_05220"
     CDS             complement(<2028..2505)
                     /locus_tag="PK977_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRF17544.1"
                     /translation="MNVLALFKPITTFVFDVDGVLTDGTVLVLDNGLQARRMHIKDGF
                     ALQMALKNGYRVKVISGGHSPQVHDRLDKLGVKDVTMSVLNKGSFLEKFIRENQLKKE
                     EVLYMGDDLPDISAMKVSGLACCPADAVNEVKDIAQYISPLNGGFACVRDVIEKVLK"
     gene            complement(2567..2806)
                     /gene="iscX"
                     /locus_tag="PK977_05225"
     CDS             complement(2567..2806)
                     /gene="iscX"
                     /locus_tag="PK977_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRF17545.1"
                     /translation="MNQFEPPIHWNDYEDIALKLYERFGDEFNEGKIYRVRFTDLLEW
                     VLQIPGFTGKREESNEGHLEMIQSSWVYEWRDNQK"
     gene            complement(2889..3224)
                     /locus_tag="PK977_05230"
     CDS             complement(2889..3224)
                     /locus_tag="PK977_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF17546.1"
                     /translation="MYTIKLNFEQKGLEPVTLTNVEEGQTLLEIALKNNIELHHNCGG
                     VCACTTCHLYIEKGMEFIDEITDREEDFIDRAVSPKLNSRLGCQSLLLEGSGEIEVTI
                     PDQTQFLGE"
     gene            complement(3297..>3781)
                     /locus_tag="PK977_05235"
     CDS             complement(3297..>3781)
                     /locus_tag="PK977_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRF17547.1"
                     /translation="MEVLSGVTEHIGVFYPLQSLRKEMNSLPDVPVYFDGSDEKAKRT
                     LELLARSIAAEKVGNAGDQDRLKLHVAAVFVSNFTNHLYALAEAYCKKEGLDFRQLLP
                     LIEETALRIREISPKDVQTGPAFRHDAQTIQKHLELLKGHPQLQQLYQQFTDSIQQAG
                     "
     assembly_gap    3782..3807
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3808..4067)
                     /locus_tag="PK977_05240"
     CDS             complement(<3808..4067)
                     /locus_tag="PK977_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF17548.1"
                     /translation="MDIVIIGSGNVAAVLGRKFKAAGHQILQIFSRNASAASALAYEW
                     DTESTNYISLINKHADVYIVAVNDDSITAVAKELLLPGKVVA"
     assembly_gap    4153..4181
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4182..4986
                     /locus_tag="PK977_05245"
     CDS             <4182..4986
                     /locus_tag="PK977_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF17549.1"
                     /translation="LLAAISKKKAAEKAFELFCTPQRRNLKQPPKIFEQAEKVQQKVE
                     GITVKGWHWHPETAAIPQRKALIIHGFESSAINFDRYVRPLLRKGYEVLAVDAPAHGR
                     SGGSKINAPLYKKTIQAINKQFGPIQSFMAHSFGGLAVCLALEEISHTPDYRMVLIAP
                     ATETTTAIETFFSFLKLDPAIRPEFDKVIIKKGGVSPAWYSIRRAMKHIKAKVLWIHD
                     EDDDTTPLSDALKVKDENHTNIQFVITKGLGHRRIYRDNKVTKQIVEFL"
     gene            4983..5681
                     /locus_tag="PK977_05250"
     CDS             4983..5681
                     /locus_tag="PK977_05250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRF17550.1"
                     /translation="MRLLLYLLVFVTSSCHKPISESMPAPTAPKKYHYLALGDSYTIG
                     EMVAPPDNFPSQLSALMSNDTVDFQPARIIAKTGWTTDELEAGIIAADQQQPLRSSYD
                     FVTLLIGVNNQYRGRTVDNYKPEFEELLNKAIRYAGNKADHVAVLSIPDWGVTPFANG
                     RDRAQIAAEIDAYNAANKQITQLYNVHYIDITPWTREAANDLSLLAADGLHPSAKEYK
                     RWAEALKQFFKTKL"
     gene            5830..8418
                     /gene="topA"
                     /locus_tag="PK977_05255"
     CDS             5830..8418
                     /gene="topA"
                     /locus_tag="PK977_05255"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRF17551.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGKDFQVKSCFGHIRDLEKAGMGI
                     DLEKNFEPRYIVPEEKEKVVNELKSLAKKSDEVWLATDEDREGEAISWHLCEVLGLDP
                     KTTKRIVFHEITKPAIQEAVQNPRKLDMNLVMAQQARRILDRIVGFELSPVLWRKMSM
                     RNNLSAGRVQSVAVRLIAEREREINAFVPVSTFKIEAAFTAKDVTDKNVTFTAEGKKQ
                     NTAEEAESFLKGCIGASYKVTDIQVKPGKRSPAPPFTTSTLQQEASRKLGYGVSRTMQ
                     IAQRLYENGYITYMRTDSVNLSNTALGDITNTVKSMYGEDYHQFRKYKNKNESAQEAH
                     EAIRPTYMSNSTVPEVEWQKLYELIWKRTMACQMADAELEKTTAKIEISTNKEELTAS
                     GEVLKFDGFLKVYREDKDEEELEEEANEGMLPPLTVGQQLPLKEMKATERFSRPLPRY
                     TEASLVKKLEELGIGRPSTYAPTISTILKRGYVEKRDKEGVRRDFRVFKLQKDSVSKL
                     MEQENTGAEKAKLFPSDLGLVVTDFLKQYFDDIMDYSFTARIEEEFDEVAEGKMKWNK
                     MINDFYDPFKKDVEKTIETAERIKGERILGTDPESGKPVVARMGRYGAMIQIGEANDE
                     EKPRFAKIPTGQSIETISFEEAMNLFGAQGTMGQYEGKDISVNVGRFGPYVKWGDEFI
                     SIPRGTDLSSVDLEKAIHYIKAKQKADTPVGNYDGKPVIKGTGRFGPYIKWDGMFINV
                     PKRYNFANLSQEEMNELIEAKIKKEENRFIHRWADEKIAVENARWGPIIKFGKKIIRL
                     PKKADDTKYTAEEAAAFTLDDVKKFIEKEVPGAFAKKGTTKKNTAKKAPAKKAAVKKK
                     AAKKKK"
     gene            8445..>8725
                     /locus_tag="PK977_05260"
     CDS             8445..>8725
                     /locus_tag="PK977_05260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF17552.1"
                     /translation="MKRSYWENMAPAYAEEIFDVLHNDKKALIRSVIKRYASKNKTVI
                     DIGCAIGKWLPVLAPAFKKVVALDISAKNLEIAAQLYPQYNNVQYLRAD"
     assembly_gap    8726..8735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8736..9194
                     /locus_tag="PK977_05265"
     CDS             <8736..9194
                     /locus_tag="PK977_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17553.1"
                     /translation="KKTKVPECDFGICINAILTPSQKERDIFFASLGNCVKKGGRIVI
                     TIPSLESHLLTSVIQRQYKIEQRYFPEEKNGKEAIRKWNNIRRGNADIDEVPHKHYLK
                     EELQLLLQKNGFKAEQFEKIEYDWTTEFHNPPGWLKDPKPWDWMVVAKKL"
     gene            9324..10232
                     /locus_tag="PK977_05270"
     CDS             9324..10232
                     /locus_tag="PK977_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRF17554.1"
                     /translation="MLPIIELVHESLPLETNKEISALFTLIDDPDEEVFGLVSSKIID
                     YGKNIIPNLEHLWETTPNEEVQNRIEMLIHRLHYRDLTEDFRQWNVSGHHDLMLGAML
                     TSKFQYPDISTTPVLQEIEKIRRNIWLELNNYLTPLEQINIVTSILYSYYGLKGGETN
                     YKEPNEFLLHKTLESKRGNQISNGILYLVLCELLDIPVRAIQIPKQFIIAYFKPGYSD
                     ESLPNPVHKIEFFIDPSSGHVFTQQDVDAYFKRISVPPVGTYFKPQKNKQVIQLLLEE
                     FGKCFTNEKESYKQAELIELAKLLDT"
     gene            complement(10242..11684)
                     /locus_tag="PK977_05275"
     CDS             complement(10242..11684)
                     /locus_tag="PK977_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF17555.1"
                     /translation="MTTENKGMNASRRKFIRNTVITGGAFFIVPRHVLGRGYIAPSDK
                     LNIAGIGVGGKGAGDLMEFAKSPYVNIVALCDVDDREAKGSRERFPKAPYYKDFRDML
                     EKEKNTIDAVSVSTPDNTHAVATLMAMQLGKHVYTQKPLTHDIYEARILTEAAKKYKV
                     VTQMGNQGGSGDGVRIAKKMVDDGLIGDVYEAHAWTNRPVWPQGIPTPKGQFDVPKEL
                     DWDLWLGPAKFTEYNPAYLPFNWRGWWAFGTGALGDMACHIMDPIYRILPILYPESAE
                     CSVSNVWKDMWAEGNYVDSCPPSSIIHLSYPRTDGKGNISVSWHDGGLLPQRPAELLP
                     EEEFGNWDGGVLMVGRKGKLLLDCYGENPRLLPTKKMKEIKIDKQNIKFVKEGHYLQW
                     VNACIAGYGKGKTSSPFEYAGPFTESILMGNLAIRSWMMLNPKRTGWGDKYQGRKKLL
                     WDAKNMKVTNFDEANQFVKREYRDGWKLEL"
     gene            11723..>11819
                     /locus_tag="PK977_05280"
     CDS             11723..>11819
                     /locus_tag="PK977_05280"
                     /inference="COORDINATES: protein motif:HMM:NF007494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRF17556.1"
                     /translation="MTINWNDFEKIDIRVGTIIEVADFPKARKPAY"
     assembly_gap    11820..11847
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11848..12085
                     /locus_tag="PK977_05285"
     CDS             <11848..12085
                     /locus_tag="PK977_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRF17557.1"
                     /translation="LKIDFGAMLGIKQSSAQITSHYNKEDLFKRQVVAVVNFPPKQIA
                     DFISECLVLGIYDTNNDVILLKPDKPTPNGQKIG"
     gene            12360..12578
                     /locus_tag="PK977_05290"
     CDS             12360..12578
                     /locus_tag="PK977_05290"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRF17558.1"
                     /translation="MSYLELARKTGDTKATRAVANANGKNNIAIIVPCHRVIGSNKEL
                     VGYAGGLWRKKWLLEHEAKVAYGVQTLF"
     gene            complement(12590..13123)
                     /locus_tag="PK977_05295"
     CDS             complement(12590..13123)
                     /locus_tag="PK977_05295"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRF17559.1"
                     /translation="MPLRKPEALLSYLLLLMEHKYEFQYEVFNDISELSEDDAWLLTE
                     AREVTEQAYAPYSNFHVGAVAILENGEVVAGTNQENASYPVGICAERVLLGSVATIHP
                     DVPIKTIAVSYNSEEVKSDHPISPCGMCRQALLEYETRTKSPIRLILSGQEGKVYIIK
                     TARFLLPFAFTSDELNK"
     gene            complement(13136..>13991)
                     /gene="lepB"
                     /locus_tag="PK977_05300"
     CDS             complement(13136..>13991)
                     /gene="lepB"
                     /locus_tag="PK977_05300"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRF17560.1"
                     /translation="DIKRKNAWGPPYNAIVFDDGSGGYTRITTGEVMNDPVNFPMVVH
                     PVDKTDNYIKRCVAVAGDWLEVKDGDIYINGEKEKTPPYSLMTYTLNTKAGSIDLDGL
                     MDKYNIKESEISFVAPNTYRAVLAHKSVEKMKADGVLVTAVPDLEKPEEVYTDSTRLG
                     KVTFPWSTNFKWTIDYFGKIWIPAKGETLKLTLENYPIYERAIRVYEKNEFYQKDGKF
                     FLNGKEVTEYTFKMDYYWMMGDNRHGSQDSRFWGFVPEDRIVGKAWLIWFSWEGGPRW
                     NRLFRIVK"
BASE COUNT         4191 a         3019 c         2730 g         3736 t
ORIGIN      
        1 cagtaaaatt tttgaaaccc ttcccttcaa ctcaattatg ggaatctcac tattcattgc
       61 atcattttcc caaaccacat gcaggatgac gttattataa tttttatcca gttggtgctt
      121 gtgcttttgc cagtcagacg ttttcagatg cagttctacc gttccggccc atattgtatt
      181 accaatttta atttttgcat cactgaagtc aggcccctgg ttggtattat gctggccggg
      241 aaagatgatt tgtaccccct ccccgttttt ggttagtaaa tctgtcttgt tgaaatattg
      301 aaattgccag atgtattgca atagccgttc ggtcatgcca ggttaaggtt taatatttaa
      361 agttataatt ccatctttaa acaacctgaa acattcaacc tcaaacatta aacttcttta
      421 gttccctcac caccctgctc acaggtaatc ccatcacatt atagaaatca ccgctgatgg
      481 attgtatgcc caccactcca atccattcct gaatggcata ggccccggct ttatcataag
      541 gtttgtattt gtcaacataa aattcaatct gtttttctgt cagatcatga aactgtacat
      601 ctgttttatc agcaaaagaa atttctttat cacccatcct gattaccacc ccggttatca
      661 cctggtgttt ttcacctgaa agagccatta atatcgtgat tgcttcttcc cggtgaaccg
      721 gcttaccaat aatatgttca ttcagcacaa caatagtatc ggctgccagg ataatgtcat
      781 cattattcct tctttgctgt accgccaaag ctttattacg ggcaatgtga atggcaattt
      841 catccgggtg aaggtccggc ggaaaatctt catcggtatc agccaccaca atttcaaaag
      901 gcacttcggc ccattccagt aattgttttc ttcttggaga ctgggaagct aatataatac
      961 tcataagtaa aaatagaaaa acaccatgga aactataccg gtcagcatga ccagtttggc
     1021 cagtgtgctg agttgatgat actgatggct tgtagaactg ctcttcaacc tgaataacaa
     1081 aagacacaag gggaaaatga tagctacaat actataagcc accnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aaacaatcgg cattgtgcga
     1261 cagccatact tttcgtcacc cggcatatcc tccatgtctt ttatcgcctc acggatcagg
     1321 gaaatgataa aagcaaaacc cgcataaagc atggccagtc ggaagaactt ataatgattc
     1381 gcaacaccag cgccgatggc atcggacaaa ttcacctttg aaaagaaaat aatcagaatg
     1441 gtccatgctg ttaacagaga gattaccaca ttcccaacca acaggctttt tttaaaattg
     1501 gtggaataaa cccagagtaa gactatacat ccagcattag ccaggataag gtaccatttt
     1561 tgcaaaaaag gcaaggcaag aatggtgaac aaaagacctg ttgtgctcag cataaannnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnattg atgatgtaac cggcggccgc aatcagcagg
     1741 gaagccagca acagaaaagc aaaatgaaga ccatcattcg ccggaatatt ctctttatac
     1801 aaaggatagt aaatgcagga ttggaacagg aactgcgtca gtgcaataaa gatgagattg
     1861 ggcagtcgca ccagtttgag gaaagcggct atcaatctca tggaaatagt ttaagatgga
     1921 aagatactac agaactttta agctaaggag aaacgctgaa tctattaccg ggaagccaca
     1981 tcctccctgt aatgccagtg gtcattcaac nnnnnnnnnn nnnnnnnact tcagtacttt
     2041 ttcgatcaca tcccgcacac aggcaaaacc tccattcagc ggcgaaatat attgtgctat
     2101 atcttttact tcatttactg catcagccgg gcaacaggca aggcctgaaa ctttcatagc
     2161 agaaatatca ggaaggtcat ctcccatgta cagcacttcc tctttcttca actgattttc
     2221 ccggataaat ttttcaagaa aggatccctt attcaaaaca gacatggtta catccttcac
     2281 ccccagtttg tcaagccggt catgtacttg tggtgaatga ccgcctgaga ttacttttac
     2341 cctgtatcca ttcttcaatg ccatttgcag tgcaaaccca tcttttatat gcatgcgtcg
     2401 ggcctgcaaa ccattgtcaa gtaccagcac cgtcccgtcg gtcagtaccc cgtctacatc
     2461 aaaaacgaat gtggttattg gtttaaagag tgcaagtaca ttcatgtttg taaagttaac
     2521 aagttcaaaa gttgaatgga aacatattaa ccttttaacc tgtcaattac ttctggttat
     2581 ccctccattc atacacccat gaactttgta tcatttcaag gtggccttca ttactttctt
     2641 ctcttttacc tgtaaagccg gggatctgca gcacccattc cagcaggtcg gtaaagcgaa
     2701 ccctgtatat ttttccttcg ttaaattcat caccaaaacg ttcatacagc ttcagggcaa
     2761 tatcttcata atcgttccag tgtatgggag gttcaaattg attcataaat ctgagtatga
     2821 tgtctgaagt ctgagcgata ataccggagg tcataatcag acttcttact tctgacctcc
     2881 tgctttttct attctcctaa aaattgtgtc tggtcaggta tggtcacttc aatctctccg
     2941 ctaccttcca gcagcaggga ctggcaacct aatcttgaat tcagttttgg acttaccgcc
     3001 cggtcaataa aatcctcttc ccggtcagtt atttcatcaa taaattccat tcctttctct
     3061 atatacaggt gacaggtagt acaggcacaa acacctccgc aattgtgatg cagttcgata
     3121 ttattcttca atgcaatctc cagcaatgtt tgaccttcct ccacattggt aagcgtaaca
     3181 ggttccagtc ctttctgttc aaaattcagt ttgatcgtat acatctcttc tctttataaa
     3241 agtggtgcaa aagtaatggt aaaacaaaaa aggcacttac ttgttcggga aaacaatcag
     3301 cctgcctgct gaatactatc ggtaaactgc tgatacagtt gctgtaattg aggatggccc
     3361 ttcagcaatt caaggtgctt ttgtatggtt tgtgcatcat gccggaaagc aggacctgtt
     3421 tgcacatctt ttggggaaat ttctcggata cgaagcgctg tttcctcgat caatggtagt
     3481 aattgtctga aatcaagtcc ttctttttta caatacgctt cagctaaggc atacaggtga
     3541 ttggtaaaat tgctcacaaa cacagctgct acatgcagtt taagcctgtc ctgatctcct
     3601 gcattgccca ctttttcagc tgcaatggaa cgggccagta attcaagggt cctttttgct
     3661 ttttcatcac tgccatcaaa ataaacaggt acatcgggca ggctgttcat ttccttacgc
     3721 agactttgca aggggtaaaa aacaccaatg tgttctgtca cgccactcaa tacttccatt
     3781 gnnnnnnnnn nnnnnnnnnn nnnnnnntgt gccaccactt ttccgggcag taataattct
     3841 ttggccactg ccgtaatgga atcatcatta acggccacaa tatatacatc agcatgcttg
     3901 ttgatcaaac taatatagtt ggttgattct gtatcccact cataagcaag ggctgatgca
     3961 gccgaagcat tgcgactaaa tatttgcaat atctggtgac ctgcagcttt gaattttctg
     4021 cccaaaactg ctgctacatt tcctgaacct atgattacta tatccattat acacctgtta
     4081 atttgtcgaa tattgtttta tcaaaataac ccgggctatt tttgtcgcat gaagttctct
     4141 caacaccttg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacttctggc ggccatatcc
     4201 aaaaagaaag cggcagagaa ggcatttgag cttttctgca caccacagcg ccgaaacctg
     4261 aaacaaccac ccaaaatatt tgagcaggca gaaaaggtac aacagaaagt agaaggaata
     4321 accgttaaag gatggcattg gcaccccgaa actgctgcta ttcctcaaag gaaagcctta
     4381 atcattcatg gctttgaatc gtcggcaatc aattttgacc gctacgtacg tccgctgctg
     4441 agaaaaggct atgaagtatt ggctgtggat gcacctgcac atggccgaag tggaggctca
     4501 aagatcaatg caccgctgta taaaaaaacg atccaggcta ttaataaaca gttcggccct
     4561 atacaatcct ttatggcgca ttcatttggc ggattggctg tttgtcttgc cttggaagaa
     4621 atcagccata cccctgacta tcgcatggtg ctcattgccc ctgccacaga aaccaccaca
     4681 gctatcgaaa cattttttag cttcctgaaa cttgatccgg ccatccggcc tgaatttgac
     4741 aaagtgatta taaaaaaagg aggcgtttca cctgcctggt attctatacg cagagcgatg
     4801 aagcatatta aggcaaaagt cctctggatt catgatgaag atgatgacac taccccgtta
     4861 agtgatgcat tgaaagtaaa agatgaaaac cataccaata ttcagttcgt aattacaaaa
     4921 ggattgggcc accgccgtat ttaccgcgac aacaaagtga ccaaacagat tgttgaattt
     4981 ttatgagatt acttttatac ctgttagttt tcgttacttc gtcctgtcac aaaccgattt
     5041 cagaaagtat gcctgcaccg acagcaccaa agaaatatca ctacctggcc ctgggtgact
     5101 cttataccat tggcgaaatg gtagcgccac ctgataattt ccccagccag ttatcggcac
     5161 tcatgagtaa tgataccgtc gattttcaac cggcaagaat cattgccaaa accgggtgga
     5221 caactgatga actggaggcg ggtatcattg ctgctgacca gcaacaacca ctgagaagtt
     5281 cctatgactt tgttacctta ttaataggtg taaataacca atacagggga agaacggttg
     5341 acaattataa acctgagttt gaagaactgc taaacaaagc aattcggtat gcaggaaata
     5401 aagcagatca tgtagccgta ctctctatac ccgactgggg agtaaccccc tttgccaacg
     5461 gaagggacag ggcacaaatt gccgccgaaa ttgatgctta taatgcagcc aataaacaaa
     5521 tcacacaatt atataatgtg cattatattg atataacacc atggaccaga gaagctgcta
     5581 acgacctgag cctgcttgcc gctgatggat tacatccttc tgcaaaagaa tacaagcgtt
     5641 gggcagaagc actgaaacaa tttttcaaaa cgaaattata gctgataatc ataggggaaa
     5701 tgccaaaaat gcaatacccc cgaaactgct gaaacaggca acaaaaattg ttttcccgaa
     5761 aaatttatta tccttgtcct aacttgcggc cgctgaaaag cggttttgta ttaatatata
     5821 gtatctctta tggcgaagaa tttactgata gttgagagcc ctgcgaaagc gaaaacgatt
     5881 gaaaaaatcc tgggaaaaga tttccaggta aagagttgct ttggccatat ccgtgacctg
     5941 gaaaaagcag gaatgggtat tgatttggaa aagaattttg agccccgcta tattgttcct
     6001 gaggaaaaag aaaaagtggt gaatgagttg aaatcgctgg caaaaaaatc tgacgaagtg
     6061 tggctggcaa cggatgagga ccgggaaggt gaagctatca gctggcattt gtgcgaggta
     6121 ttaggccttg atccgaaaac aaccaaaagg atcgtatttc atgagattac aaagcctgct
     6181 atacaggaag ctgtacaaaa tccccggaag ctggatatga acctggtgat ggcgcagcag
     6241 gcaagaagaa tactggaccg catcgttggt tttgaattga gccctgtgct ctggcgaaaa
     6301 atgagtatgc ggaacaacct gagtgcaggc cgtgtacaga gtgtggcggt tcgtttgata
     6361 gcagaaagag aaagggagat caatgctttt gttccggtca gtacttttaa aattgaagcg
     6421 gcatttacgg ccaaagatgt gaccgataag aatgtaacct tcaccgcaga aggtaaaaaa
     6481 caaaataccg cagaggaagc agaatcgttt ttaaaaggct gtatcggcgc cagctataaa
     6541 gtaacggata tacaggtaaa accgggaaaa cgttcccctg ctccgccttt tactacctcc
     6601 acattacaac aagaagcatc ccgaaaactg ggatacggtg tatccagaac catgcagatt
     6661 gcacaacggc tgtatgagaa cgggtatatc acttatatgc gtacagacag tgtgaacctg
     6721 agcaacactg cactgggaga tattaccaat acagtaaaaa gtatgtacgg cgaagattat
     6781 caccaattcc gcaagtataa gaataaaaat gaaagtgccc aggaagctca cgaagcgata
     6841 aggccaacct atatgagcaa ttcaacagtg ccggaagttg aatggcaaaa attatatgaa
     6901 ctgatctgga aaagaaccat ggcctgccag atggctgatg cagaactgga aaaaacaact
     6961 gctaaaattg aaatttcaac caataaagaa gaactcacag ccagtggcga agtattgaag
     7021 tttgacggct tcctgaaagt ttaccgggaa gataaagatg aagaagaact ggaggaagaa
     7081 gccaatgaag gtatgctgcc accgcttacg gttggacagc agttgccatt aaaagaaatg
     7141 aaagctacag aaaggtttag ccgccccctt cctcggtata cagaagcttc gctggtaaaa
     7201 aaactggaag agctgggtat cggtcgtcct tctacatatg cacctaccat atccaccatt
     7261 ctgaaaagag gatatgtgga aaaaagggat aaggaaggtg tgcgccgtga ttttagagtg
     7321 ttcaaactac aaaaagactc agtaagcaaa ctgatggagc aggaaaatac cggtgctgaa
     7381 aaagcaaagc tgtttccttc agacctgggt ttggttgtaa cagacttcct gaagcaatac
     7441 tttgatgaca tcatggatta tagcttcaca gcccgtatcg aagaagagtt tgatgaagtg
     7501 gcggagggca agatgaagtg gaataaaatg ataaatgact tctacgatcc ctttaaaaaa
     7561 gatgtagaaa aaacgataga aactgctgaa cggattaaag gagaaaggat ccttggtaca
     7621 gatccggaaa gtggtaagcc tgtagttgcc cgtatgggac gctatggcgc catgatacag
     7681 atcggggaag ccaatgatga ggaaaagcca cggtttgcta aaataccaac cggccagagt
     7741 attgagacca tcagctttga agaggccatg aacctttttg gggcacaagg taccatggga
     7801 cagtatgaag ggaaagatat ttcagtgaat gtcggtcgtt ttgggcctta tgtaaaatgg
     7861 ggcgatgaat ttatttccat tccaagaggc accgatcttt catccgtaga tttagaaaaa
     7921 gccatacatt atattaaggc caaacaaaaa gcggatacgc ctgttggtaa ttacgacggc
     7981 aaacctgtta tcaagggcac cggccggttt ggtccctaca tcaaatggga tggtatgttc
     8041 atcaatgttc ccaaacgtta caactttgcc aacctgtcgc aggaggaaat gaatgaactg
     8101 attgaagcca agatcaaaaa agaagaaaac cgtttcattc atcgctgggc agatgaaaaa
     8161 atagctgtgg aaaatgcccg ctggggtccg atcattaagt ttggcaaaaa gatcatccgt
     8221 ctacccaaaa aagcggatga cacaaagtat acagccgaag aagcagccgc atttacattg
     8281 gatgatgtga agaagtttat tgaaaaagaa gtaccgggtg catttgcaaa aaaaggaaca
     8341 actaaaaaaa atactgccaa aaaagctccg gctaaaaaag cagcagtgaa gaaaaaggcg
     8401 gcaaagaaga agaagtaatc tgttcctcta aaaaccaaaa actgatgaaa cgttcctact
     8461 gggaaaatat ggcccctgct tacgcagaag agatattcga tgtgctacat aatgataaga
     8521 aagcattgat ccgttccgtc atcaaaaggt atgcttcaaa aaacaaaaca gttattgata
     8581 ttggttgtgc cattggcaaa tggctgcccg tattggctcc cgcatttaaa aaagtagtag
     8641 cgttagatat ttctgcaaag aaccttgaaa tcgcagcgca actataccct cagtacaata
     8701 atgttcaata cctgcgggct gatatnnnnn nnnnnaaaaa gacaaaagtt cctgaatgtg
     8761 attttggtat ttgcatcaat gccatcctca ctccatcaca aaaagaaaga gatattttct
     8821 ttgcttcgtt ggggaattgt gtaaagaaag gcggtcgtat tgtcataaca atcccttcgc
     8881 tggaatcgca cctgcttacc agtgttattc agcgtcaata taaaattgaa caacggtatt
     8941 tcccggaaga aaaaaatggt aaagaagcca tcagaaaatg gaataatatc cggagaggca
     9001 atgcagatat tgatgaggta ccgcataaac actacttgaa agaagaactg caattgctac
     9061 tgcaaaaaaa tgggtttaaa gcagagcagt ttgaaaaaat agaatacgac tggaccacag
     9121 aatttcacaa ccctcccggt tggttgaaag atcccaaacc ctgggactgg atggtggtag
     9181 ctaaaaagtt gtgagttgat agctgtcaga acctctactg acaaatctcc ggcttctatt
     9241 agaatgaaaa gattaaaatc gatttgttta gcactaatca tcaccaacta ttccccactt
     9301 gtggaaaagt ttaactattt catgtgctgc ctattattga acttgtgcat gaatcgttac
     9361 cattggaaac aaataaagaa atatcagccc ttttcaccct tatcgatgat cctgatgaag
     9421 aagtgtttgg attagtctcc agcaaaatca ttgattacgg aaagaatatc atccctaatc
     9481 ttgaacacct ttgggaaacc acccccaatg aagaagtgca gaacaggatt gaaatgctga
     9541 tacaccggct gcattacagg gatcttactg aagatttcag gcaatggaat gtttccggcc
     9601 atcatgacct gatgctcggc gcaatgctga ccagcaagtt tcaatatccc gatatctcaa
     9661 caacacctgt tttgcaggaa atagaaaaaa tccgccgcaa tatctggctt gaattgaata
     9721 attatcttac tccgctggag cagatcaata ttgttaccag tattttatat agttactatg
     9781 ggttaaaggg aggagaaacg aattacaaag agcctaacga attcctgttg cacaaaacac
     9841 tggaatcaaa acgaggcaac cagatcagca atggtatctt gtaccttgtt ctttgcgaac
     9901 tgcttgatat tccggtaaga gccattcaaa tccctaaaca attcatcatc gcatatttta
     9961 agccgggtta ttctgacgag agtcttccta accctgtaca taagatcgag ttctttattg
    10021 atccatcttc cgggcatgta tttacccagc aggatgtgga tgcttatttc aaacgcattt
    10081 ctgtaccgcc ggttggtacg tatttcaagc cgcagaaaaa caaacaggtt atccagttat
    10141 tgctggaaga atttggtaaa tgctttacca atgaaaaaga aagctataaa caagccgaat
    10201 taattgaatt agcgaaatta ctcgatacct gacaagttac tttataattc cagtttccaa
    10261 ccatcacggt attctctttt tacaaattga ttggcttcat caaagttggt aaccttcatg
    10321 ttctttgcat cccaaagtaa ttttttccgg ccctggtatt tatcacccca accagtacgt
    10381 tttgggttca gcatcatcca gctccggatg gcaaggttgc ccataaggat actttctgta
    10441 aaaggtcctg catactcaaa tggtgaactt gttttaccct taccataacc ggctatacat
    10501 gcattaaccc attgcaggta atggccttct tttacaaact tgatattttg cttgtctatt
    10561 ttaatttctt tcattttttt ggttggcaac agccttgggt tctcgccgta acagtcaagc
    10621 agcaattttc cttttcggcc aaccatcaga acacctccat cccagttgcc aaattcctct
    10681 tccggtaaaa gctctgcagg tcgttgtggc aataaaccac cgtcatgcca tgacacagat
    10741 atattccctt ttccgtcagt tcggggatag gacaaatgta taatggagga tggcggacaa
    10801 ctgtccacat aattaccttc cgcccacata tccttccata cattgctaac actgcattct
    10861 gcagattcag gataaagaat gggtaaaata cggtagatag gatccataat atgacaagcc
    10921 atatcaccaa gggcacctgt tccaaatgcc caccagcctc tccagttaaa aggcaaataa
    10981 gccgggttat attcggtgaa cttagcaggt cccagccaaa gatcccagtc aagctctttg
    11041 ggcacatcaa attgaccctt tggtgtgggt ataccttgcg gccatacagg cctgtttgtc
    11101 caggcatgag cttcgtatac atcaccaatc aatccgtcat ccaccatctt ttttgcaatc
    11161 cttacgccat cacctgagcc accctggttg cccatctgtg ttactacttt atactttttt
    11221 gctgcttctg tcaatattct tgcttcataa atatcatggg tcaatggttt ctgggtgtac
    11281 acatgttttc ccagttgcat tgccattaat gtagccacag catgggtatt atctggtgta
    11341 gaaacagaga ctgcatcgat agtatttttt tctttttcca gcatatccct gaaatccttg
    11401 taatagggtg ctttcgggaa acgttctctt gatcccttcg cttccctgtc atccacatca
    11461 cacaatgcta caatgtttac ataaggactt ttggcaaact ccatcaggtc gcctgcacct
    11521 ttaccaccta caccaatacc tgcaatattc agcttatcac tgggagcaat ataacccctg
    11581 cccaaaacat gccttggcac aatgaaaaaa gcgcctccgg taatgacggt gttacgaata
    11641 aattttctgc gggaagcatt cataccttta ttttctgtgg tcattctgca aatttttatc
    11701 aagtttacag taaaataaca agatgaccat caactggaat gattttgaaa aaattgatat
    11761 ccgagtgggc actatcatcg aagtagccga tttccctaaa gcaagaaagc ctgcctacan
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt aaaaatcgat ttcggagcaa tgctgggcat
    11881 caaacaatct tccgcacaaa tcacttcaca ttacaataaa gaagatttat ttaaaagaca
    11941 ggtagtggcg gtagtcaatt tcccgcccaa acaaatagcc gattttatca gtgaatgcct
    12001 ggtactaggc atatatgata ccaacaatga tgtaatttta ctgaaacccg ataaaccaac
    12061 ccccaacgga caaaagatcg gttaaggatg agtgagttat ttcatacata ttatcattcc
    12121 ccggttggct tactcaaaat aagcggaaca gaaaactata tttccgaagt tacctttcat
    12181 gatacttccc aaaaacccga gggaaataag aaacacttac cccacctact gatcaactgt
    12241 gtggagcaac ttatccagta tttcaacgaa taacgtttga tttacccatt aatcaaccgg
    12301 gaaccgcctt tcagcaggat gtatggaata ttcttacgca gataccgttt ggtaaaacca
    12361 taagctacct ggaactggca agaaaaacag gggatacgaa agcgacaagg gctgttgcga
    12421 atgcaaatgg taagaataat attgcgatta ttgttccctg tcaccgggta atcggatcta
    12481 acaaagagtt agtaggttat gcaggtggcc tatggcgaaa aaaatggttg ctggagcatg
    12541 aggccaaagt agcctatggg gtgcaaaccc ttttttgatt ttctatctct cacttattca
    12601 actcatcgct tgtaaacgca aatggcagca ggaaacgggc tgttttaatg atatagacct
    12661 ttccttcctg cccgcttaga ataaggcgaa ttggactttt cgttctcgtt tcatattcca
    12721 gtaaggcctg ccggcacata ccgcagggag aaatcgggtg atcgcttttt acttcttcac
    12781 tattgtagct gacagcaatc gtttttatgg gtacatccgg gtgtatagta gccactgaac
    12841 ccaataatac cctttctgca caaattccca ccgggtaaga tgcattttcc tggttggtac
    12901 cagccactac ctccccattc tccaatatgg ccacggcacc aacatgaaaa ttagagtaag
    12961 gagcataggc ctgttcagtc acttccctgg cctctgtcag caaccaggca tcatcttcac
    13021 tcaattcact gatatcatta aatacctcgt actgaaattc gtatttgtgt tccattagta
    13081 aaagtaaata agacaataat gcctctggtt ttcgcagagg catttaaaga tactactatt
    13141 ttacaatcct gaacagcctg ttccaccggg gccctccttc ccagctaaac catatcagcc
    13201 aggctttgcc tactattctg tcttcaggta caaagcccca aaaacggctg tcctgtgatc
    13261 catgcctgtt gtcccccatc atccagtaat aatccatttt aaaagtgtac tctgttactt
    13321 cctttccatt caggaaaaac tttccatcct tctgatagaa ttcatttttt tcatatacac
    13381 ggatagccct ttcatatatg gggtaattct ccaatgtcaa tttcagggtt tcacctttag
    13441 ccggtatcca tattttacca aaataatcaa tggtccattt gaagtttgta ctccagggaa
    13501 aagtgacttt gcccaaacgg gtggaatcag tataaacttc ttccggcttc tccaggtcag
    13561 gaacggcagt aaccaacact ccgtcagctt tcattttttc tactgattta tgcgctaaca
    13621 cagccctgta agtattgggt gccacaaaac taatttcact ctccttaata ttgtacttgt
    13681 ccattaatcc gtcaagatct atgctcccgg cctttgtatt cagtgtatat gtcatcaatg
    13741 aatacggtgg tgtcttttct ttttcaccgt taatataaat gtcgccgtct tttacttcca
    13801 gccaatcacc agccacagcc acgcaacgtt tgatataatt atcagtttta tcaaccgggt
    13861 gcaccaccat aggaaaatta acggggtcat tcattacttc tcccgttgta atacgggtat
    13921 atcctccgct gccatcatcg aaaacaattg cattataagg aggtccccat gcatttttac
    13981 gcttaatatc a
//
LOCUS       DAORLS010000045        13974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2335_length_13974_cov_1.834543, whole genome shotgun sequence.
ACCESSION   DAORLS010000045 DAORLS010000000
VERSION     DAORLS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13974
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_13974_cov_1.834543"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1157)
                     /locus_tag="PK977_05305"
     CDS             complement(15..1157)
                     /locus_tag="PK977_05305"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HRF17561.1"
                     /translation="MLLTKSYDYIFTGAGCAGMSLLMRLISSGLISGKRVLLIDRDEK
                     TRNDRTWCFWEKGDGFFEPVVYRNWKSIGFFSSDYNAQMEIAPYCYKMIRGADFYQYC
                     FNEFNKHPNIEFITGEVNFTNSGNSFQLYLDNSIIDTSESIVFNSIYEPSPPTAKTLH
                     VLQHFKGWLITSATDVFDPGKATMMDFRVHQRHGTSFVYVLPFNKQEALVEYTLFTPS
                     LLKPEEYDNELKSYLSDFLGITDYSIKDEEFGIIPMTNEKFHFRKNGWQIGTAGGQTK
                     ASSGYTFQFIQKQSQAIVDCLLTGKPLESIPSTPGRFRFYDNTLLYILYHGKLPGDKI
                     FSRLFKKNKPRQVLRFLDNESSLAGELKIISTLPTWPFLKAALKQF"
     gene            1303..2031
                     /locus_tag="PK977_05310"
     CDS             1303..2031
                     /locus_tag="PK977_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17562.1"
                     /translation="MSDTMIAIGGCHVAGYKVGEENSFVNVITKATGFILSHRAPQYQ
                     IKKTIGIREKINNCHPEIVLLQLGNHEFNASLKQILKKKENNIPGENQYVIYKEDNLS
                     KKPYMLPVVSKRKTGFFLKNLAAPLIWTYLAYKNFKHLNNIRHIIKENPGTRFVILSP
                     IPCLNASDNYIRRKAAGWFKWIFGGLSNVKYIDLFRYIAVDKRYFEDAAHLNTTGHRI
                     LGRIVSQYIKTIKKDSQKPLAVAV"
     gene            complement(2045..2479)
                     /locus_tag="PK977_05315"
     CDS             complement(2045..2479)
                     /locus_tag="PK977_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene hydroxylase"
                     /protein_id="HRF17563.1"
                     /translation="MHGFLWYLHKDHHQVRPGIFEKNDLFAFIFAVPSFLLIYFGTYD
                     HKWWLQAIGFGIMLYGFAYFLVHDVIIHQRFKWFSRSNHTYVRAIRWAHKMHHKHLGK
                     EHGESFGFLFVHKKYWDKVRKDKKLMAGSQRVEYAPDKAQQV"
     gene            complement(2579..3118)
                     /gene="idi"
                     /locus_tag="PK977_05320"
     CDS             complement(2579..3118)
                     /gene="idi"
                     /locus_tag="PK977_05320"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRF17564.1"
                     /translation="MDLTKVILVDELDNAVGSCEKLAAHKEGLLHRAFSVFIFNSKGE
                     MLLQQRAMEKYHSGGLWTNACCSHPAEGEDLASAAHRRLKEEMGFDTDVEKVFDFVYK
                     ASFENGLTEYEFDHVFIGYYDEAIHANREEVMDYCYKSLDEIRASLSTHPHRYTAWFQ
                     LAFPKIEQWWLLRQAQSVE"
     assembly_gap    3218..3233
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3234..3968)
                     /locus_tag="PK977_05325"
     CDS             complement(<3234..3968)
                     /locus_tag="PK977_05325"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRF17565.1"
                     /translation="MVQLFHTTSENCSRIVTENYSTSFSSAIRMLHKDLRTPIFNIYG
                     FVRFADEIVDTFHDYDKQTLLAQFRKDTYDAIERGISLNPVLNSFQRTVNEFKIDLKL
                     VDAFFHSMEMDLDKKCYDSKGYKEYIYGSAEVVGLMCLYVFCEGKKELYDKLEYSARA
                     LGAAFQKVNFLRDIKADYNGMSRVYFPGCDFANFTEKDKQEIEEDIQKDFKEAFEGIR
                     QLPMKARFGVYVAYKYYLSLFKKIKRL"
     gene            complement(3985..5463)
                     /gene="crtI"
                     /locus_tag="PK977_05330"
     CDS             complement(3985..5463)
                     /gene="crtI"
                     /locus_tag="PK977_05330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRF17566.1"
                     /translation="MTNRKSVIVIGSGFAGLSAASFMAKEGWEVTVIEKNSTPGGRAR
                     QWKEQGFTFDMGPSFYWMPDIFERYFAQFGKKVSDYYQLERLDPSYRVYWDNGFTDMP
                     ADFASLKKTFDSIEPGSGEQLEKYLQGAAYKYDVGINKLVYKPGHSITEFMDWPTISG
                     IFKLQVFSSIKKHISSYFKNHRLRQLMEFPVLFLGALPEDTPALYSLMNYADIKGGTW
                     YPKGGMYAVVEGMYKLANELGVKFLFSESVEEIKVDRSSVKRVVTDKAVYEADAVISG
                     ADYHFTETKLLPEASRSYSDAYWQKKVMAPSCLLYYVGLNKKLNNPVHHSLFFDVPFD
                     QHGKEIYKEPGWPTEPLFYVSVSSATDSSVAPTGCENIVFLIPVAAGLSGDDEAVREK
                     YFTMIARRMEKHLGEPILDSVVYKKSYSVSDFVLDYNSFRGNAYGLANTLWQTAIFRP
                     ACQSRKVKNLFYTGQLTVPGPGVPPSLISGEVVAKQVIKSIR"
     gene            complement(5472..>5922)
                     /locus_tag="PK977_05335"
     CDS             complement(5472..>5922)
                     /locus_tag="PK977_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF17567.1"
                     /translation="KPFAINLTRDTEAANDLYQETLYKALANQEKYNVGTNIKAWLFT
                     IMRNIFINNYRRKAKQKAIFDNSANDFLLNQKQATVGNAAEGSLRMKEIQEAIQQLPE
                     IFKTPFLLYFDGYKYNEIAEALNEPLGTIKSRIHFARKLLKEQLSRY"
     assembly_gap    5923..5951
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6165..6334
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6335..>6885)
                     /locus_tag="PK977_05340"
     CDS             complement(<6335..>6885)
                     /locus_tag="PK977_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HRF17568.1"
                     /translation="AIQERIINELVQEMVDMNMERFEKILNNYIASRGIERTVTQIIF
                     PFLEKIGILWLTGHINPAQEHLVTNIIRQKLIVAIEGTISHISMDKTFLLFLPEGEHH
                     ELGLLYMYYLLKNRGAKTIYLGANVPVKDVEYVINAKKPDIIFIHLTATASNFNFEKF
                     LTNVQLRFGQVKTIISGQLTQQY"
     assembly_gap    6886..6907
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6908..7143)
                     /locus_tag="PK977_05345"
     CDS             complement(<6908..7143)
                     /locus_tag="PK977_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRF17569.1"
                     /translation="MNAFTIKDLENLSGIKAHTIRIWEQRYNFLKPQRTTTNIRYYSN
                     DELKTILNIALLNKYGFKISHIDRMDHQEMQQRIL"
     gene            complement(7323..8066)
                     /locus_tag="PK977_05350"
     CDS             complement(7323..8066)
                     /locus_tag="PK977_05350"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HRF17570.1"
                     /translation="MLFFRNKKAKELVDYGFIAVDMHSHLLPGIDDGSPDMTTSLELI
                     KGMQSLGYSKLVTTPHILSDMYPNTREGILAKLVEVRQAMREAGMNAELHAAAEYFLD
                     DHVAGLLKNKEPLLTIHANWVLCEFSLAFPSHSLKDILFELQMQDYQPVIAHPERYIY
                     LQQSKDFYDELKATGCLFQLNVLALAGHYGKAVQELAQYLVRKGYYDLLGTDLHHFKH
                     LEALSDAGLVTQLRKLVDTGKIRNGEMVG"
     assembly_gap    8172..8192
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8193..>8994)
                     /locus_tag="PK977_05355"
     CDS             complement(<8193..>8994)
                     /locus_tag="PK977_05355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF17571.1"
                     /translation="VPVIVHTYHGHVFHSYFNSAKTRAFIGIERYLGKRSNAIIAISE
                     QQHKELVTDYRIAPAEKFRIVPLGLDLDKFASGNDEKRKSFRSSYGIPDDTIVITITG
                     RMVPVKNHELFLQGISYLLQHSPKKIKAFIVGDGETRTAMESRAKDLGIGFSTEKDNL
                     HDEPLVFTSWRSDIDVINAGSDIITLTSFNEGTPVSLIEAHAAGKPVVSTRVGGIEAV
                     VDEKITGFLSNINDVQGYCDNLMELVNNDILRQQFGEVASAKVKEKYS"
     assembly_gap    8995..9020
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9021..9332)
                     /locus_tag="PK977_05360"
     CDS             complement(<9021..9332)
                     /locus_tag="PK977_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF17572.1"
                     /translation="MPRVLRILNRLIVGGPLLNASYLTKYMEPEFETMLVVGEKESHE
                     KDASYFTDQLGIKPVYVPEMGRSISPLKDYGAYKKIKQLIREFKPDIVHTHAAKPGAI
                     GR"
     gene            complement(9484..>10097)
                     /locus_tag="PK977_05365"
     CDS             complement(9484..>10097)
                     /locus_tag="PK977_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HRF17573.1"
                     /translation="ERYRRWAGFMDETSSSRMIVWDELTLPQAQGEILNRKKDILYRL
                     EGGEAMEDFLATDMGLVLLSDMLVKVDRMSMANSLEVRSPFLDHKVVDFAFSLPTSYK
                     IDKGMKKKIVQDTFRKYLPAELYNRPKHGFEIPLLDWFRKELKGKITDDLLNDRFVEE
                     QGLFNAEEVRRLKQRLFSSNPGDAHATVWALMVFQHWWKRNTG"
     assembly_gap    10098..10153
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10154..>10442)
                     /locus_tag="PK977_05370"
     CDS             complement(<10154..>10442)
                     /locus_tag="PK977_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRF17574.1"
                     /translation="RHTVFSLTNNDFLEHIDAVMNSIDEPFADSSAIPVYILSKHTRS
                     AVTVALSGDGGDEVFAGYNKHAAELRMRRRSLLNSAVKAGGPLWKLLPQSRN"
     assembly_gap    10443..10511
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10512..11384)
                     /gene="asnB"
                     /locus_tag="PK977_05375"
     CDS             complement(<10512..11384)
                     /gene="asnB"
                     /locus_tag="PK977_05375"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRF17575.1"
                     /translation="MCGITGIISFSPAGKDRLSKVKVSADTLFHRGPDGGNYFTDDIC
                     ALGHRRLSIIDPSSNGAQPMKDPSGRYVLIFNGEIFNYQQLKQQYFPDKQDWQSQSDT
                     EVLLHLYIQQKERCLPLLGGFFAIAIYDVQEHELFLARDRYGKKPVYYHQSPAAFFAF
                     ASELKALQDYGISRQLDMASLQQYLQLNYIPEPYSIYKDVKKLRPGHYMKIKAGVAAE
                     HAYYELDWRRTDTTISYQAACNQLEQLMDASVQGRMISDVPLGAFLSGGIDSSVVVAL
                     ASRYTQQLNTFSIGY"
     gene            11414..>11839
                     /locus_tag="PK977_05380"
     CDS             11414..>11839
                     /locus_tag="PK977_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17576.1"
                     /translation="MQYIEQYSWISLVDFLLLPFYLAIFYKIAYSFRDKYYKKGHPWR
                     NYFIPALSYKIIGAIFISFIYVYYYEGGDTTNIYDNAYYINKIFSESPRTWLELITRT
                     ISKNDPVFFNYADGIYFYKDPATFNVCILTSFVNVLTLKS"
     assembly_gap    11840..11855
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11856..>12340
                     /locus_tag="PK977_05385"
     CDS             <11856..>12340
                     /locus_tag="PK977_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17577.1"
                     /translation="FLISAIFFAFISFWGVWAMFRAFATLYPNLTRPMAYAALFIPST
                     IVWGSGIFKDTVCLFALGWLFNSTVQMVVLKKFGPKVILIAVLSFLLLVQIKLYIVMS
                     FLPALLLWIFFYYSRSIRNSYARQLAKFVVIGIVAVSAIVGLSYFSSQLGKYSLDNIV
                     KT"
     assembly_gap    12341..12441
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12442..12808
                     /locus_tag="PK977_05390"
     CDS             <12442..12808
                     /locus_tag="PK977_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17578.1"
                     /translation="GGMIAKFPAAVNVTLFRPYPWEAKKPIVALSALEALLFLFLTLK
                     IFFEVGLKKIWATISRDPTIQFSLVFTLIFAFAVGISSYNFGTLTRYKIPCMPFYAAA
                     LIVIYYENKAGKKKLLPFL"
     assembly_gap    12871..12895
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12896..13630)
                     /locus_tag="PK977_05395"
     CDS             complement(<12896..13630)
                     /locus_tag="PK977_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HRF17579.1"
                     /translation="MKIYITNLKSSTERRRHMQKECAKENLEYSFFDCVVGSELQDTE
                     IAEKCDIETISRYKNKEHWLNKGIIGCTLTNQNVYRDIISNDYPFALYLEDDIKFANG
                     FKQLLIKIGALVQPGDVILLNYLPKRTLELKPVKILIDNYGLYGVENPEAVGGGAAFV
                     VTRDAAEKMLLFNTPIRITPDCWGDYKKASAIKRLLCVYPMPVTGIFVQSTMQIGRFL
                     KLRNFINQYRIFPFYGMIKLYKRIKRK"
     gene            complement(13650..>13974)
                     /locus_tag="PK977_05400"
     CDS             complement(13650..>13974)
                     /locus_tag="PK977_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hexapeptide transferase"
                     /protein_id="HRF17580.1"
                     /translation="VFIGKGVTILSLSVIESTVRIGNGCLLNLNTIVTHDTIVGDFCE
                     IGPGVRISGSCTIGDNVFIGTGAVLLPKIKVGSNSIIGAGSVVVDDIPGNTTVVGVPA
                     RPVSG"
BASE COUNT         3903 a         3133 c         2407 g         3980 t
ORIGIN      
        1 acccggacca ataatcaaaa ctgcttcagc gctgccttta aaaaaggcca ggtgggaagt
       61 gtagaaataa ttttcagttc tcctgccaaa gaactttcat tatccaggaa cctcagcact
      121 tgccggggtt tatttttttt aaacaagcgg ctgaatatct tatcacctgg cagcttaccg
      181 tgataaagaa tatacaatag tgtattatcg taaaaccgaa aacgtcccgg tgttgatggt
      241 atactttcga ggggcttacc agtcagcagg caatcaacaa tagcctggga ttgcttttgt
      301 ataaattgaa acgtatatcc gctggaagct ttggtttgtc ctcccgcagt accaatttgc
      361 cagccatttt tcctaaaatg aaatttttca ttggtcatgg ggatgattcc aaattcttcg
      421 tctttgattg agtaatcagt aatacctaag aagtctgaaa ggtaactttt taactcattg
      481 tcatattctt ccggttttag cagtgatggg gtgaacaagg tatactccac caatgcttcc
      541 tgcttattaa atggcagcac atatacaaaa ctggttccat gccgttggtg tacccggaag
      601 tccatcatgg tggctttgcc cgggtcaaaa acatcagtag ctgacgttat taaccagcct
      661 ttgaaatgct gtaatacatg taaagtcttt gcggttggtg gagaaggctc atagatcgaa
      721 ttaaacacaa tactttctga tgtatcaata attgaattat caaggtaaag ttgaaaacta
      781 ttgccggaat tggtaaagtt aacctctcca gttatgaact ctatattcgg atgtttattg
      841 aattcattaa aacaatactg gtaaaaatca gcacccctta tcatcttgta acaataaggt
      901 gcaatctcca tttgcgcatt ataatcactg ctgaaaaaac caatactctt ccagttgcga
      961 taaacaactg gttcaaaaaa cccatctccc ttttcccaaa aacaccaggt cctgtcgttc
     1021 ctggttttct catccctgtc aatcagcaat actctcttcc cggatattaa accactactt
     1081 atcagccgca tcaaaagact cattcccgca caaccagcgc ctgtaaaaat atagtcatat
     1141 gattttgtca ataacataaa cgattgaatc aaagataata agacaagaac atgaaaaaat
     1201 acttcgtggt tttacgtaat aaaaacgggt actgttgctc tttgttcagg ctaccaggca
     1261 atttactttt acaagagatc caaactatta aaaactgaac ctatgtccga tactatgatt
     1321 gccattggcg gctgccatgt agctggttat aaagtggggg aagaaaactc ctttgtgaac
     1381 gtaataacaa aagctacagg tttcatactt tcacatcggg cgccacaata ccagattaaa
     1441 aaaaccattg gcattaggga aaagataaac aattgtcatc cggaaatagt gcttctgcaa
     1501 ttgggaaatc atgagtttaa tgcatcgctt aagcagatcc tgaaaaagaa agaaaacaat
     1561 atacccggag aaaaccaata tgtcatttat aaagaggaca atctttctaa aaagccatat
     1621 atgttacctg ttgtcagcaa acgaaagaca ggcttttttc taaaaaacct tgcggcacca
     1681 ttaatatgga cttatttggc atataaaaat ttcaaacatc tgaataatat caggcatatt
     1741 attaaagaaa atcccggaac caggtttgta atactttcac ccataccttg cttaaatgct
     1801 tccgacaatt acattaggcg taaagccgca ggctggttca aatggatatt tggaggatta
     1861 tcaaacgtaa aatatattga tctattcaga tatattgctg ttgacaaaag atactttgag
     1921 gatgccgcac atttaaatac cacaggccat agaatactcg gaagaattgt aagccaatat
     1981 atcaaaacga taaaaaaaga ttcacagaaa cctttggctg tggctgttta atactatttt
     2041 caaatcagac ctgctgcgct ttatcagggg catactccac ccgctgactt ccggccatca
     2101 gctttttatc ttttcgtact ttgtcccagt acttcttgtg tacaaaaaga aatccgaaac
     2161 tttctccatg ctccttaccc aaatgcttat ggtgcatctt gtgtgcccag cgaatagccc
     2221 taacataagt gtgattgctg cgggaaaacc atttaaaccg ttgatgaatg atcacatcat
     2281 gtaccaaaaa ataggcaaaa ccatacaaca tgatgccaaa gccgatggcc tgcaaccacc
     2341 atttatgatc ataagtacca aaatagatta gtaaaaaact gggaaccgcg aaaataaagg
     2401 cgaaaaggtc attcttttca aaaataccgg gccttacctg gtgatgatct ttatgcaaat
     2461 accacaagaa tccatgcatg acaaatttat gggtacacca tgtgatacct tccattatga
     2521 caaacgtacc caacagaacc agaatataat aagcgacctg cataacacta taactttttt
     2581 attcaacgga ctgagcctgc ctgagcagcc accattgttc tattttagga aatgccagct
     2641 gaaaccaagc agtataacgg tgagggtgag tggataaaga agccctgatt tcatcaagac
     2701 ttttatagca gtaatccatt acctcctccc tgttagcatg aatggcttca tcgtaatagc
     2761 ctataaaaac atgatcaaac tcatactctg taaggccatt ttcaaacgag gctttataaa
     2821 caaaatcaaa aactttttct acatcggtgt caaaccccat ctcttccttg agtcggcggt
     2881 gtgccgcact tgccagatcc tctccctctg caggatgact gcaacaagca ttggtccaca
     2941 agcctccgct atggtatttt tccatagctc gttgctggag aagcatttca cccttactat
     3001 tgaaaataaa aacactgaaa gcccggtgca gcaaaccctc tttatgtgcg gctaattttt
     3061 cgcaactgcc aacggcatta tccagctcat ccaccagtat cactttcgtc aggtccatat
     3121 cagatcaggc gcaattgatt ttttacccct gcccggaaaa caatcatagc ctttcggtag
     3181 tcaggtatcc tgatgcgggc atccaatacc cttgcagnnn nnnnnnnnnn nnncagtcgc
     3241 tttatttttt taaacaacga gagataatat ttataggcca catacacacc aaaccttgcc
     3301 ttcatgggca attgacggat accttcaaat gcttccttga aatctttttg aatatcttct
     3361 tcaatttcct gtttatcttt ttctgtgaaa ttggcaaaat cacaacccgg gaaatataca
     3421 cggctcatgc cattgtaatc ggctttgata tcccgcagaa agttcacttt ctgaaaagct
     3481 gcgcccaatg ccctggcaga atattccagc ttatcatata gctctttctt gccctcgcaa
     3541 aaaacataca ggcacatcaa acccactact tctgcagaac catatatata ttctttatag
     3601 cctttggaat cataacattt tttatccagg tccatttcca tgctgtgaaa gaaagcatcg
     3661 accagtttca gatcaatttt aaattcattg accgtacgct gaaagctgtt caataccggg
     3721 ttcaggctta tacctctctc aatggcatca taagtgtctt tccggaattg tgccagcagt
     3781 gtttgcttgt cataatcatg aaaagtatca acgatctcat cggcaaaccg aacgaaccca
     3841 tatatgttga aaatgggtgt acggagatct ttatgcagca tacggatggc agaagaaaag
     3901 gacgtactgt agttttccgt cacaatgcgg ctgcaatttt cgctggtagt gtgaaaaagt
     3961 tgtaccataa ttgaatataa gcaattatct gattgattta attacttgtt tagctactac
     4021 ttcaccactg atcaggctgg gtggtacacc cgggccgggt acagtcagtt gtcctgtata
     4081 aaataaattc ttcactttcc tgctctggca agctggacga aaaatagctg tttgccataa
     4141 agtattggcc agcccataag cattgccgcg gaaagaatta tagtccagta caaaatcact
     4201 caccgaatac gattttttat atactaccga atcaagaatt ggctctccca ggtgcttttc
     4261 catcctccgg gcaatcatcg taaaatattt ttctcgtacg gcttcatcat ccccgctgag
     4321 cccggctgca accggtatca ggaatacaat attttcacaa cctgtaggcg ctaccgaact
     4381 atcagtagca gatgaaacac ttacataaaa caaaggttca gtaggccagc ccggttcttt
     4441 ataaatttct ttcccatgct ggtcaaatgg gacatcaaag aacaaagaat gatgaactgg
     4501 attattgagc ttcttgttca gccccacata gtaaagtaaa cacgaaggcg ccatcacctt
     4561 cttctgccag taagcatcag agtaactgcg ggaagcttcc ggcaacaact tcgtttctgt
     4621 aaaatgatag tccgcgccgc tgatgacagc atctgcttcg taaacagcct tatcagtcac
     4681 cactcttttt acgctggacc ggtcaacctt tatttcctca accgattctg aaaaaagaaa
     4741 ttttacaccc agttcattgg ccagcttgta cattccttcc acaaccgcat acattccacc
     4801 ctttggatac caggtaccac ctttaatatc ggcataattc atcaggctgt acagggcagg
     4861 agtatcttcc ggcaaggctc ctaaaaataa aacaggaaac tccatcagct gacgaagccg
     4921 gtggtttttg aagtaagaag aaatatgttt ttttatcgat gaaaaaacct gtagtttgaa
     4981 aatgccactg atggtgggcc agtccataaa ttctgttatc gaatgacccg gtttatacac
     5041 cagtttatta atacccacat cgtatttata agcagccccc tgcaagtatt tttccaactg
     5101 ctcgccgctg ccgggttcaa tgctgtcgaa tgtttttttg agtgatgcaa aatcagcagg
     5161 catatccgta aatccgttat cccaatacac cctatacgac ggatctaatc tttccagttg
     5221 ataataatca ctgacttttt taccaaactg ggcaaaatag cgttcaaaaa tatcaggcat
     5281 ccagtaaaaa ctgggaccca tatcaaatgt aaaaccctgc tccttccatt gccgggcacg
     5341 gccaccgggt gtgctgttct tttcaataac agttacttcc cagccttctt ttgccataaa
     5401 agaggcggca gacaatccgg caaaaccgct gcctattaca attactgatt tccgattagt
     5461 cattatgatt tttaataccg gctgagctgc tcttttagca atttacgggc aaaatgaata
     5521 cggctcttga tagttccgag aggttcattt aatgcctcag caatttcatt gtatttgtaa
     5581 ccatcaaaat acaggagaaa gggtgtttta aatatttcag gcaattgttg aatggcctcc
     5641 tgtatctctt tcatccggag actaccttca gcagcattac caacagtggc ctgtttctgg
     5701 ttcagcagaa aatcattagc actgttgtca aaaatggctt tttgtttagc ttttctgcga
     5761 taattgttaa taaaaatatt ccgcatgata gtgaaaagcc atgccttgat attggtgcct
     5821 acattgtatt tttcctggtt cgccagagct ttgtacaggg tctcctggta caggtcattg
     5881 gccgcttccg tatcccttgt aaggtttata gcaaaaggtt tcnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn ntcagaaagt ctgcattgtt gaccagcatt tggttaaact cagttgtaga
     6001 catatgtatg tgtttaataa ttgaagtgta aaattaaaca caataatgac gattaaccaa
     6061 atattttgtt caatttattg ataaaatttt taaacaaaat agtttccaac aaaaaaagcc
     6121 tgatttccag gcttttaata aaattcaatc ccttgaatct ttacnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatattg ctgggtaagc tggccggaaa
     6361 tgatagtttt cacctgtccg aaacgtaact gaacattagt gagaaacttc tcaaaattga
     6421 aattggaagc tgtcgctgtc aggtgaatga aaataatatc gggctttttg gcattgatca
     6481 catactctac atctttaacc ggtacattgg caccaaggta aatggtcttg gcacccctgt
     6541 tcttcaatag gtaatacata tacagcaatc caagttcatg atgctctcct tccggtaaaa
     6601 acaaaagaaa ggttttatcc atgctgatat gcgatatggt tccctcaata gccactatga
     6661 gtttctgccg tatgatatta gttacaagat gctcctgtgc aggattgata tgcccggtaa
     6721 gccataagat gccgattttc tctaaaaaag gaaatatgat ctgcgttact gttctttcaa
     6781 taccccggga agcgatataa ttattcagga ttttttcaaa ccgctccata ttcatatcca
     6841 ccatctcctg caccaactca ttgataatgc gttcctggat ggcctnnnnn nnnnnnnnnn
     6901 nnnnnnnagt atccgttgtt gcatctcctg gtggtccata cggtcaatat gggatatttt
     6961 aaacccgtat ttattgagca atgcaatatt cagaattgtc tttaattcat cattgctgta
     7021 gtagcggatg ttggtagtgg tacgctgggg ctttaaaaaa ttgtaccgct gttcccagat
     7081 acggatcgta tgggctttta taccagaaag gttttccagg tctttgatgg taaatgcatt
     7141 cataatagca tagttacaac cggccaaaaa aaaagttcag ccaaaattga ctgaactttt
     7201 tcaaaaaagt aaaacccggc cctttcaacc cctgtcaccc tgcctgcccc gacgaatgtc
     7261 gtgggagctt gtagaagggc aaccatatta aaccaccttc agcctcaacc ttaatcttat
     7321 cctcacccca ccatctcccc attcctgatc ttaccagtat caactaactt tctcaactgc
     7381 gtaaccaatc cggcatcact caatgcctcc aggtgcttga aatgatgcaa atccgttcca
     7441 agcaaatcat aatatccttt cctgaccagg tattgtgcaa gttcctgcac ggcctttcca
     7501 taatgaccgg ccaacgccag cacatttaat tggaacaaac aaccggttgc tttcagctcg
     7561 tcataaaaat cttttgactg ctgtaaataa atataacgct ccgggtgggc aatcaccggc
     7621 tgataatctt gcatctgcaa ctcaaacagg atatccttta agctatgcga tgggaatgca
     7681 agggaaaatt cgcagagtac ccaattagca tggatcgtaa gtaagggttc tttgttcttg
     7741 agcaatccgg ccacatgatc atccaggaaa tattcagctg ctgcatgtag ctcagcattc
     7801 attccagctt cacgcatcgc ttgccgtacc tcaaccagtt tggccaatat gccctcccgg
     7861 gtattgggat acatatccga caaaatatgc ggtgtggtga ccagttttga atagcccagt
     7921 gactgcatcc ctttgattag ttccagtgag gtagtcatat caggcgaacc atcatcaatt
     7981 ccgggcagca ggtgcgaatg catatccaca gcaataaagc cataatccac cagctcctta
     8041 gctttcttat ttctaaaaaa caacattaat caggatctta atattcttaa ccttaatcta
     8101 aacctcaata ttaacctaag cctttcgttt tcagcaaatc ccaatacaat cccttcatat
     8161 ccctcaccag cnnnnnnnnn nnnnnnnnnn nntaactgta tttctctttc acttttgccg
     8221 aagccacttc accaaattgc tgccgcagta tatcattgtt caccagttcc atcaggttat
     8281 cacaatatcc ctgcacatca ttgatattcg acaaaaagcc cgttattttt tcatccacta
     8341 cagcttcaat accacccacc cttgttgata ccactggttt acccgcagca tgtgcttcaa
     8401 taaggcttac cggcgttcct tcattaaaag aagtaagtgt aataatatca gaaccggcat
     8461 tgattacatc aatatcacta cgccacgatg taaataccaa tggttcatca tgcaagttgt
     8521 ctttctccgt agaaaaaccg atacccaggt cctttgccct tgattccata gcagttcttg
     8581 tttctccgtc acctacaata aaagccttga tcttcttcgg ggaatgctga agcaggtagg
     8641 aaataccctg taagaacagc tcatggttct tcaccggcac catccttccg gtaatggtga
     8701 tcacaatagt atcatcaggt attccatatg aggaacgaaa tgattttctt ttctcatcat
     8761 taccgctggc aaacttatcc aggtctaatc ccagcggaac aatgcgaaac ttctcagcag
     8821 gtgcaatacg gtaatcagtt accagttcct tatgttgctg ttcactgatc gcaatgatag
     8881 catttgatct ttttcctaag taccgttcaa tgccaataaa tgcccttgtc ttggctgaat
     8941 tgaaataaga atgaaacaca tggccatgat aggtatgtac aatcaccggc acctnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn cctgccgata gctcccggct tggccgcatg ggtatgcaca
     9061 atatccggtt taaactcccg gatcagttgt tttatttttt tgtaagcacc ataatctttc
     9121 agtggactga tggaccggcc catctcaggc acatacactg gtttgatacc taactggtca
     9181 gtgaaataac tcgcatcttt ctcatgcgac tctttctctc ccactaccag catcgtttca
     9241 aactccggtt ccatgtattt cgtcaggtac gaggcattca gcaacggccc tcctacaatc
     9301 aaacgattca atatccgtaa tactcttggc ataaataata ttcaacttat atcacctttg
     9361 caatttgtca tgctgagcct gtcgaagcat gcaaacttct cagtcatcag tcttcgacaa
     9421 gctcagactg acatttaacc cctcaaccat cctcaaccgt tcaactcctc aacactaaac
     9481 ccctcaacct gtatttcttt tccaccagtg ctgaaacacc atcaatgccc acaccgtagc
     9541 atgtgcatca ccgggattgg aagaaaacaa tcgttgcttc agccttctca cttcttccgc
     9601 attaaacaat ccctgttcct ccacaaaccg gtcattaagc agatcatccg tgatctttcc
     9661 cttgagttct ttgcggaacc agtcaagcag cggaatctca aaaccatgct tgggacggtt
     9721 atacagttct gccggtaaat atttccgaaa agtatcctgc acaatcttct tcttcattcc
     9781 cttatcaatc ttataggaag taggtaacga aaaagcaaaa tcaaccacct tgtggtcaag
     9841 gaaaggactc cgcacttcca ggctgttggc catactcatc cggtccacct tcaccagcat
     9901 atcactcagc agcaccagcc ccatatccgt tgccaggaaa tcttccattg cctcgcctcc
     9961 ttctagcctg taaaggatgt ctttttttct gttaagaatt tcaccttgag cctgtggaag
    10021 ggtgagttca tcccatacta tcattcggct tgatgaagtc tcgtccataa accccgccca
    10081 ccgcctgtaa cgttcttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnntattcct cgactgcggc aataatttcc ataatggtcc ccctgcttta
    10201 acagcactat tcagcaacga acgacggcgc atccgtaatt ctgccgcatg tttattatac
    10261 ccggcaaaca cttcatcacc gccatcaccg gataaagcta ctgtaacggc gctccgggta
    10321 tgcttactca gtatatatac cggtatagcc gatgaatcag caaaaggttc atcaatgctg
    10381 ttcatcaccg catcaatatg ctccaggaaa tcattgttgg tcaacgaaaa aacagtatgc
    10441 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn natagccaat cgaaaaagta tttaactgct gtgtataacg gctggccagc
    10561 gctaccacta cggaagaatc aattcctccg cttaaaaaag cgcctaaggg cacatcgctg
    10621 atcatccttc cctgtaccga tgcatccatt aattgctcta attggttaca ggctgcctga
    10681 taagaaatag ttgtatctgt cctgcgccag tctaattcgt aataagcatg ttcagccgca
    10741 acgcctgcct taatcttcat ataatgaccc ggccgcagtt tcttcacatc tttataaata
    10801 gaatacggct caggtatata attcagttgc aggtattgct gcagcgatgc catatctaac
    10861 tgcctggaga taccataatc ctgcaaagcc tttaattctg atgcaaaggc aaaaaatgct
    10921 gcaggtgatt gatgataata aacaggtttc ttgccatagc ggtcacgggc caggaacaac
    10981 tcatgctcct gcacatcata gatggcaata gcaaaaaaac caccgagcag cggcaggcat
    11041 cgttctttct gttgtatata caggtgcagc agcacttctg tatccgactg cgactgccag
    11101 tcttgtttat ccggaaaata ttgctgtttt aattgctgat aattaaaaat ttcaccgtta
    11161 aagatcagca cataccggcc tgatggatct ttcatcggct gggcgccatt gctggaagga
    11221 tcaataatgc taagccggcg atggcccaat gcacatatat catcagtaaa gtaattaccc
    11281 ccatccggcc cgcggtgaaa cagcgtatcg gcagaaacct ttacttttga aagccggtct
    11341 tttccggctg gtgaaaaact gataattcct gtgataccac acatacttct tattttctaa
    11401 ttgttgatcc ctgatgcaat atatagaaca gtatagctgg atttcattgg ttgacttcct
    11461 gttgctccca ttttaccttg ctatatttta caagatagcg tatagtttca gggataaata
    11521 ttacaaaaag ggtcatccct ggcgaaacta ttttatcccg gccctttcct ataaaattat
    11581 tggcgctata tttatttcct ttatttatgt ttattattat gaagggggcg acactacaaa
    11641 tatttatgat aatgcttatt atattaataa aattttcagc gaatcgcccc gaacatggct
    11701 ggaactgata acacgaacca tttcaaaaaa tgaccctgtt ttttttaatt atgctgacgg
    11761 tatctacttt tataaagacc cggcaacatt taatgtatgt attctcactt catttgtcaa
    11821 tgtactgact ttaaaaagcn nnnnnnnnnn nnnnntttct catctctgcg atatttttcg
    11881 cctttatttc cttttgggga gtttgggcca tgttccgggc ttttgccaca ctttatccca
    11941 acctgacaag gccaatggcc tatgcggcgc tctttattcc cagcactatt gtttggggat
    12001 caggaatttt taaagatact gtctgcctgt ttgcgttagg ttggttattc aatagcaccg
    12061 tccagatggt tgttctaaaa aaatttggcc ccaaagtgat actcattgca gtacttagct
    12121 ttttgctgct ggttcaaatc aagttatata tagtaatgag cttcctcccg gccctgctgc
    12181 tttggatatt cttctattat tcccgtagta ttcgtaacag ttatgccagg caactggcaa
    12241 aattcgtggt tattgggatc gttgccgttt cagccatcgt agggcttagc tatttttcca
    12301 gccagttggg taaatactcg ctggataata ttgtcaaaac nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn ntggcggtat gatcgctaaa tttccggcag ccgtcaatgt
    12481 aacccttttc aggccctatc cctgggaagc caaaaaaccg attgttgcat tatcagccct
    12541 cgaagcttta ctctttttat tcctgacatt aaaaatattc tttgaagtgg gactaaaaaa
    12601 gatttgggct actatttcaa gggacccgac aatacagttc tcgcttgttt tcactctcat
    12661 ttttgccttt gctgtcggca tctcctccta caattttggc acactgaccc gttataagat
    12721 tccctgcatg ccattctatg cagccgcatt gatagttatc tattacgaaa ataaggctgg
    12781 gaaaaagaag ttgttgccct ttttataagg tactgtccct ggttacgggt tcaggaaatg
    12841 caacacaacc aaaattgtat ttattatttt nnnnnnnnnn nnnnnnnnnn nnnnntttac
    12901 gctttattct tttgtaaagt tttatcatgc cataaaaggg aaatattcgg tattgattaa
    12961 taaaatttct aagctttaaa aacctgccga tttgcatagt ggattgaaca aaaatgcctg
    13021 tcaccggcat tgggtataca caaagcaatc gcttaatggc agaggctttt ttataatcac
    13081 cccaacaatc cggggtaatc cttatcggtg tattaaacaa tagcattttt tctgcagcat
    13141 cccttgttac aacaaaagct gccccacccc caactgcttc tgggttttca actccataga
    13201 gcccataatt gtcaataaga attttgactg gctttaattc aagtgttctt ttcggcagat
    13261 aattcaacaa gatcacatct cctggctgga ccaaagctcc gatcttgatt aatagttgtt
    13321 taaatccatt cgcaaatttg atatcatctt ctagatatag tgcaaaggga taatcattag
    13381 aaatgatatc cctgtaaaca ttctgattcg ttaatgtgca gccgataatt cccttgttca
    13441 accaatgttc tttgttctta taccggctga tagtttcgat atcacatttt tcggctattt
    13501 cggtatcctg caattcgctg cctactacgc aatcaaaaaa tgaatattcc aggttctctt
    13561 tggcacattc tttttgcata tgccttcttc tctccgtact gctcttcaaa tttgtaatgt
    13621 aaatcttcat tgcaacaatt ttctccatat tacccggaga caggcctggc aggaacccca
    13681 actactgttg tattgcccgg gatatcatcc acaacaactg aacctgcacc gatgatactg
    13741 tttgaaccta cttttatttt gggaagcaag acagcccctg tccctataaa aacattatcc
    13801 ccgattgtac atgatcccga aatcctgacc ccgggaccaa tttcacaaaa atcaccaaca
    13861 atggtatcat gagtcacgat agtatttaag ttaagtaaac aaccattgcc aatgcgtact
    13921 gtgctttcta tgacagataa tgaaagaata gtcacccctt ttccaataaa aacc
//
LOCUS       DAORLS010000046        13746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2385_length_13746_cov_2.020451, whole genome shotgun sequence.
ACCESSION   DAORLS010000046 DAORLS010000000
VERSION     DAORLS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_13746_cov_2.020451"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PK977_05405"
     CDS             <1..533
                     /locus_tag="PK977_05405"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HRF17581.1"
                     /translation="DFSKTGGLFQMVQLWVNLPAAYKKTKPKYQEITNDMMGKAFLKD
                     NKSYVEVIAGEYNGAKGPASTFTPMHVYNVKMKKGASATMNLPSHYNTGFLVIEGNVT
                     MNDSSVATDHFVLFKNDGEEIIIEAMEDAVVLVLSGEPINEPIAAYGPFLMNTWAEVE
                     QAIQDVNAGKFGVLEE"
     gene            complement(578..>977)
                     /locus_tag="PK977_05410"
     CDS             complement(578..>977)
                     /locus_tag="PK977_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF17582.1"
                     /translation="IIFSDPAFGLLKGDEVKYLWEMLCTNAKDFSLTFSNIQLLDEEY
                     ATCNWAATYTFSKTGRKVVNRIKAFMKLKDGKIIEHSDAFRLSTWAAQALGLKGQLFG
                     WMGWMKRKIQQNARKSLIQFIEKKQNSVVS"
     assembly_gap    978..1006
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1251..2820
                     /locus_tag="PK977_05415"
                     /pseudo
     CDS             1251..2820
                     /locus_tag="PK977_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
     assembly_gap    2821..2874
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3026..>3850
                     /locus_tag="PK977_05420"
     CDS             3026..>3850
                     /locus_tag="PK977_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF17583.1"
                     /translation="MQKIIFFILTLIIQSSVFTQERQLNLIPWPVEVKQESGTYSLKK
                     VMTVSNSSTAIGWEILFPYLRDEMKKQFGITLIEAEKGKTGDIDFFMKRMPTSGKPAY
                     TLDVGMNGIKINSNFDEPAFHAVQTLFQLITVDQKGKREMPYVHIFDHARFNYRGMHL
                     DVGRHFFPVSFVKKYIDYLAYHKLNTFHWHLTEDQGWRIEIKKYPELTKVGAWRNGTI
                     IGRYPGTGNDNTRYGGYYTQEEIKEVVQYAKERFIEVIPEIEMPGHSSAAIAAYPWL"
     assembly_gap    3851..3899
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3900..>4288
                     /locus_tag="PK977_05425"
     CDS             <3900..>4288
                     /locus_tag="PK977_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF17584.1"
                     /translation="DTAGQRIKLVQETWGVFDDVFCAGNDSTFIFLQNVIDEVVELFP
                     SNFFHIGGDECPKTHWKQCARCQHRMKENNLKDEHELQSWFVQKIEKYLNSKRKTLIG
                     WDEILEGGLAPNAVVMSWRGETGGIEEA"
     assembly_gap    4289..4317
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4318..5394
                     /locus_tag="PK977_05430"
     CDS             <4318..5394
                     /locus_tag="PK977_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF17585.1"
                     /translation="IMTPGNPVYFDHSQSENEDSITIGGYNPIEKVYAYEPVPAALTA
                     EQGEYVLGSQANVWTEYMKNTKKVEYMIFPRMAALSEVLWSTKENKNWQNFERRLINH
                     FKRYEKWGANYSKAYYDIKSSILPGEKDDHVLWKLESKQKGITIKYITHYAGRPYPST
                     FSEGIIYKSPLEINSTKLVAAIGMLGNKAISNPVYGTFRFNKATGKKITLTAPPSKSY
                     PGDGAFTLVNGVQNEKGLAKRNEFLGFSGADCEAIIDLGKDEKIAMVTVHALHQTGSW
                     IWRPLSAEVHTSTDGSKYEFAGLTDDFNVTIKGLEKGKMKISFDKKEARFVKIIVKCW
                     EEIPAGEPGAGKKPWLFIDEIEIN"
     assembly_gap    5593..5617
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5618..>7099
                     /gene="nagB"
                     /locus_tag="PK977_05435"
     CDS             <5618..>7099
                     /gene="nagB"
                     /locus_tag="PK977_05435"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRF17586.1"
                     /translation="KQKKKEKCVLGLATGSTPKSLYAELVRLHREEKLSFKNVITFNL
                     DEYYPIDNNALQSYNRFMKNQLFDHIDIDPKNIHIPNGEIKKEDIKEHCRQYEKMIKD
                     AGGIELQILGIGNNGHIGFNEPGSGIYSKTRLITLDNSTRIANAYEFANISQVPRLAI
                     TMGISTILQSKKIILMAWGSAKAPVIKKAVEDDDTEQVPASLLQNHDDVTFVVDESAA
                     AELTRNKSPWLTGDCEWTPKMIKKAVVNMALKLKKPVLSLTNSDYNEYGLSDLLVEKG
                     DAYEINLQVYYMLRDSITGWPGGKPDSHIPNHPERSAPYPKRVVIFSPHPDDDIISMG
                     GTFQRLHDQGHDVHVAYQTSGNIAVTDEFVTRFLDFAVGFEEIVGIDTKKSGKILDDA
                     RRFIARKKSNQVDTPAIRSIKGLIRRCEAKATCRYVGIKEENIHFQNLPFYETGTIEK
                     KPMGEKDVKLTMELLRKIKPQQVYCAGDFADPHGTHIVCFNVVV"
     assembly_gap    7100..7141
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7142..7434
                     /locus_tag="PK977_05440"
     CDS             <7142..7434
                     /locus_tag="PK977_05440"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRF17587.1"
                     /translation="YEWINDCWLWLYKGAWQEWNIEEIEMAIPMSPDQVLKKRFGIFI
                     HQSQKDMVPFQGSDSREFWQRAEERNAGTANLYADLGLTHYAAMEAFVRWQY"
     gene            7484..>7674
                     /locus_tag="PK977_05445"
     CDS             7484..>7674
                     /locus_tag="PK977_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17588.1"
                     /translation="MSIFKSSNPVMQEKTYQGTILEGISTGEEMTLKGTMNKFGVMIM
                     LMIGSTLFAWSQFYKGSDP"
     assembly_gap    7675..7685
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7686..>8051
                     /locus_tag="PK977_05450"
     CDS             <7686..>8051
                     /locus_tag="PK977_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRF17589.1"
                     /translation="LMMIGVFGGLALALVMAFKMKWASFLAPAYAILEGLFVGSISAI
                     YDYAYPGLPMQAVALTLLVTLIMFLIYRYKIIKVTSKLRTVIVVATSAVAIFYLIQWL
                     TYAIGGTAIAYNFTNSATP"
     assembly_gap    8052..8061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8274..8441
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8442..>8654
                     /locus_tag="PK977_05455"
     CDS             <8442..>8654
                     /locus_tag="PK977_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRF17590.1"
                     /translation="EPSWKEVLKDEFSKPYFQQVVLHLKTEKSQGKVIYPPGPYIFNA
                     FNITPVDHIKVVILGQDPYHGPGQAH"
     assembly_gap    8655..8728
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8729..9108
                     /locus_tag="PK977_05460"
     CDS             <8729..9108
                     /locus_tag="PK977_05460"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRF17591.1"
                     /translation="HDDIGVEIPNHGNLTHWAEQGVFLLNASLTVRAGEPMSHAKIGW
                     ATFTDTVIRRISDLKAHVVFMLWGKFAQEKKILIDETKHLVLKAAHPSPLSAHAGFLG
                     SRHFSKANEWLISKGIDPVDWKL"
     gene            9125..9862
                     /locus_tag="PK977_05465"
     CDS             9125..9862
                     /locus_tag="PK977_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF17592.1"
                     /translation="MKLLKDIFGRFWAFWGIISFIGTFLVIYIPSMITWLLPEPKGQR
                     IFIGIARIWMNIWLRLVGCPVKIKGRQNFQRGKSYIVTCNHNSLMDVPLSSPFIPGAN
                     KTIAKSSFAKVPLFGFYYMKGAVLVDRKSEKSRKESFEKMKLVLKKGMHMCIYPEGTR
                     NRTNEPLKKFYDGAFKLATDTGTDIIPAIILNTRKAVPVHKSFYFLPHKLEMHFLPPV
                     SVENKETEELREEVFVIMKKYYLEHQH"
     gene            complement(9859..10575)
                     /locus_tag="PK977_05470"
     CDS             complement(9859..10575)
                     /locus_tag="PK977_05470"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRF17593.1"
                     /translation="MVFQQGYNFLRDSLKLDAFNIYLRSTLFDKIQINAQTVLDPYQT
                     DKYGRNINKYAWQGGKFKLGRLTYGSVSVNTSFKSKPKDPKKDELRKKQQEELLSDPS
                     LAGDQRRLLDYMQQNPGDFVDFNIPWQVTLGYSISFTTRLSADLVNFEKDFSSGLNFN
                     GSFNLTPKWNFSAQGNYDFDTKEMQTFSMSINREMHCWQMSINVTPIGLYRSFSFSIS
                     PKAAVLQDLRINRSRFFTNF"
     gene            complement(10553..12484)
                     /locus_tag="PK977_05475"
     CDS             complement(10553..12484)
                     /locus_tag="PK977_05475"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRF17594.1"
                     /translation="MAYADSSSYFYKPLTDTTRPVNPSDSGRKILSLVTDTTKLPVID
                     TSLKPKTDTFSLKLSKDSLEAPLKYEAADSAVVLIQDKKIILYGKTKTEYKDITLTAP
                     KVEVDQVTQIVTAVNAKDSTGTVTETAHFKSGESEFTSDTIRYNFKTQVGLTKNTYTQ
                     QGEFLVIGEVAKKVSENTTFIKRARFTTCLLDDPHFAFVTPQMKVINQKLAVSGPAHP
                     EFEGVPVPIYLPFGFYPLSQGRHSGLLPPRFATNEQFGLGLEGLGYYKVISDYWDVKV
                     YGNIYSYGGWSANVNPSYRKRYRYNGAFNLSVQSTRFNFKGDPDYLKNKSYMVNWTHS
                     VDSRARPGTTFSASVNAGSTKFNQLVPNNVQLNFQNQMSSSIAYSKSWAGKPYNLTIS
                     ANHNQNNRTRLINISLPDAGFAVNTLYPLQKKNATGTAKWYEKLGIAYNGNFRNQVSF
                     YDTAFKLKNLIDTLQWGAQHSFPVTLALPPILGGAVIVSPGFSYSQVWIAQKFRRRWN
                     SATQKVDTSITKGFFMDHQAQFSLSFNTAIFGTYQFKKSRIVAIRHVIRPTFSLNYKP
                     DLSRKFYYTDTVATGVTQRFSVFEGALYAGYGEGKTGGIGFQLDNNLEMKMRSKKDTG
                     ENAIKKVKLIDGYGFSTGL"
     gene            12667..13707
                     /locus_tag="PK977_05480"
     CDS             12667..13707
                     /locus_tag="PK977_05480"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF17595.1"
                     /translation="MIKRTLVFFLFISFGVTVFSFSEKEEKKKGKQKVINTIVIDPGH
                     GGSDAGAHGVYSYEKDICLDISLKLGKMIEKEFPDIKLLYTRTTDTYPALHSRANFAN
                     ENKGDLFLCIHVNSAPAKRMSEITGYKTVTYYTGKGKNRKKKTKQVPQYRYYTVPSNA
                     KGTETYIWGAHKSEEKELAVRENSYIYMEKDFEKNYGPNFDPNSPEAVAVSLLKTKQY
                     FKRSATLAGYVQDEFAKVGRVDRDVKQRGVGIWVLQATAMPSVLVETGFISNPEEEKY
                     LNSQEGQTEIADCIVKALRKYVKWLEDKQEDDKEKDEKKDDGSPQSAGLNRDNREFLE
                     MVDKMEKQRMSK"
BASE COUNT         4167 a         2576 c         2844 g         3668 t
ORIGIN      
        1 aagatttcag caaaaccggt ggcctttttc aaatggtgca attgtgggta aatcttcccg
       61 cagcttataa aaagacaaag cccaaatacc aggaaatcac aaatgatatg atgggcaagg
      121 cctttttgaa agataacaaa agttatgttg aagtgatagc cggggaatac aatggtgcaa
      181 aaggccctgc ttcaaccttt actcctatgc atgtttataa tgtgaaaatg aaaaagggag
      241 cttctgcgac aatgaatttg ccttcacatt ataacacagg gttcttagtg atcgaaggta
      301 atgtaactat gaacgacagt tcagtagcta cagatcattt tgtgttattc aaaaatgatg
      361 gagaagagat aattattgaa gctatggaag atgcagttgt gttagtgtta tctggagaac
      421 ctatcaatga acctattgca gcttacggtc cttttctaat gaacacatgg gccgaagtag
      481 aacaggctat acaagatgtg aatgcaggta aattcggagt gctggaagag tgaggagccc
      541 cggttattag ttaaaagttg atgctcactg cagactatta actaactact gaattttgtt
      601 tcttttcaat aaactgaatt aaactcttac gggcattctg ctgaattttt cgcttcatcc
      661 accccatcca tccaaacaac tgacctttca aacccaatgc ctgggcggcc caggtgctta
      721 atcgaaaagc atcactgtgt tctatgatct ttccgtcttt tagtttcata aaagccttga
      781 tgcgattgac aacttttctg cctgttttcg agaacgtata agtggccgcc caattacaag
      841 tagcatattc ttcatccagc aactgaatgt tagaaaaagt cagcgaaaaa tcctttgcat
      901 ttgtacagag catctcccac aaatatttca cttcatcccc ctttagcaag ccaaacgccg
      961 gatcgctgaa aataatannn nnnnnnnnnn nnnnnnnnnn nnnnnncata gatttatgat
     1021 ctctgtcgcg gaaggccgaa tagaactttg taataatatc tgcattcgtt tgcataaaat
     1081 aaattaaggt aaagaggaat cagtaaattt aaaccttcaa aatgacttac atgcgaaaat
     1141 tactcctgct ctgttatttc ctttgtttat ttattggttt agttgcccaa accgatcagg
     1201 attcattatg gatcagggaa aattacacga aaacagaaca gtatatccct atgcgggatg
     1261 gaataaagct ttttaccaca atttattcgc caaacgataa aagtgaaaag cacccgatat
     1321 tgatgaaccg taccccctac tcctgtgcgc cttatggaaa agacttcagt gcccggttat
     1381 ggagtactca ctggaaatat tattgcaggg agaattacat tatcgttatt caagatgtcc
     1441 gtggacgttg gatgagtgaa ggggaatttg tggatgtccg tccatttaat aaacagaaaa
     1501 aaggaaacga agtagatgaa gccagtgaca cttaggatgc tattgactgg ctggtaaaaa
     1561 actggcccga taataatgga aatgcaggtg tttttggcat ttcttatccg ggtttttatg
     1621 ctaccatggc agcagcatcc ggacatcccg cattgaaagc ggtaagtccg caggcccccg
     1681 ttacagattg gttcatcggt gatgattttc atcacaacgg tgcttttttt caaatggatg
     1741 gatttagttt ttattcaggt tttggtaaac cccggcctaa accgactact gttggtgcca
     1801 gggggttcga ctttcccata agagataatt acaagtttta cctggaaaac cctccttcca
     1861 agctgatgga gttgatgaga gacagtgtgc agttctggaa agacctctat tcacatccca
     1921 actatgatga ctggtggaag gcaagggatg cccgaagggc tatgtttaat attaaacctg
     1981 ccatgctggt agtaggtggt ttatttgatg cagaggactg ctggggtgca tggaatttgt
     2041 acaagtcaat cgaaaaacaa aaccccggta ttcataataa gattgtgatg ggcccctgga
     2101 gccatggcca gtggagttca agggatggaa actctctcgg caatatccga ttcaacagca
     2161 atacagcttt ttggtaccag aataatattg agttaccctt tttcaactat tacctgaaag
     2221 gaaaaggaga agatccgcaa ctggcagaag caacagtatt tttcaccggt gaaaatgagt
     2281 ggaaaaagtt agcaaagtgg cctgcttcca attcagcaag ggatatttac ctcgcacctg
     2341 acggggcttt gtcatggaat ctgccaactt atgtaaaagc aggaagtttt actgaatatg
     2401 tcagcgaccc tgcacacccg gtaccttata cggaagatgt acactttcgg cggacaagag
     2461 agtatatgac agatgaccag cggtttgctt cgaggaggcc ggatgtatta acattccaaa
     2521 cctatccgct tgaagaagat cttactttag ccggtcctgt cattgcaaat ttaaaagtca
     2581 gtattagcag cacagatgcc gactttgtcg taaaagtgat tgatgttttt cctgacgatt
     2641 tcagatatcc cggttctttt caatctgcgg gccgtactgc cggcggcgat tatcccatgg
     2701 atggttacca gatgctggta agaggagaaa taatgcgggg caaattcaga gagagttttg
     2761 aacatccagt tcctttcaaa cccggaaaaa ttgagacagt aaaatttgag ctgccggatg
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctggt
     2881 ttccgctggc cgaccgtaat ccgcaacaat tcatgaatat ttacgaagca aaaccagccg
     2941 attataaaaa agcaacgatt cggattcatc atgatggcaa taatccttca tcgatttact
     3001 taccaattat gaccaaataa taaatatgca aaaaatcatt ttttttatac ttacactaat
     3061 tattcaatca agtgtattta cacaagaaag gcaattaaac cttattccct ggccagtgga
     3121 agtaaaacaa gaatcaggca catattcact aaaaaaagta atgactgttt cgaattcttc
     3181 aacagccatt ggatgggaaa tattatttcc atacctgagg gatgagatga aaaaacaatt
     3241 tggtattact ttgattgagg ctgaaaaagg taaaaccggt gatatcgatt tttttatgaa
     3301 acgcatgcct acctcaggca aaccggctta tacactggat gtgggtatga atggtattaa
     3361 gatcaacagc aatttcgacg aaccggcttt tcatgcagta caaaccttgt ttcagttgat
     3421 tactgttgat cagaaaggaa aaagggagat gccttatgtg catattttcg atcatgcacg
     3481 gtttaattac cgtggtatgc acctcgatgt aggccgccac ttttttcctg tatcattcgt
     3541 aaaaaaatat attgactatc tcgcctatca taagctcaat acatttcatt ggcacctcac
     3601 cgaagaccag ggctggcgga ttgaaataaa aaaatacccg gagctgacga aagttggcgc
     3661 ctggagaaat ggtaccatca ttgggcgcta tcccggcaca ggcaatgata atacccgtta
     3721 tggtggttat tacacacagg aagaaatcaa agaagtggtg caatatgcca aagaacgctt
     3781 cattgaagtg ataccggaaa ttgaaatgcc aggtcattct tctgcggcaa tagcagccta
     3841 tccctggctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3901 acacagcggg tcagagaata aagttagtcc aggaaacatg gggtgttttc gacgatgttt
     3961 tttgcgccgg caatgattct acctttattt ttttgcagaa tgtgattgat gaagtagtgg
     4021 agctttttcc atccaatttt tttcatatcg gcggggatga atgtcctaag acacattgga
     4081 aacagtgcgc caggtgccag catcggatga aagagaacaa cctgaaagat gaacatgaat
     4141 tgcaaagctg gtttgtgcag aaaattgaaa agtacctgaa cagcaaacgc aaaacactta
     4201 ttggatggga tgaaatattg gaaggtggtc ttgcacctaa tgcagttgtg atgagctggc
     4261 ggggcgaaac cggtggtatt gaggaagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata
     4321 atgactccag gcaatcctgt gtattttgat cattcacaga gtgaaaatga agatagcatt
     4381 actataggcg gctacaaccc aattgagaaa gtgtatgctt acgagcctgt gcctgctgct
     4441 cttacagcag aacagggaga atatgtactc ggctcccagg ccaatgtttg gacagaatac
     4501 atgaagaata caaaaaaagt ggagtatatg atctttcctc gcatggcggc acttagtgag
     4561 gttttgtgga gtacaaaaga aaataagaac tggcagaatt ttgaaagaag actgattaat
     4621 catttcaaaa gatacgagaa atggggagct aattatagta aggcgtatta tgacataaaa
     4681 agtagcatat tacccggaga aaaagatgat catgttcttt ggaaactgga gtcaaaacaa
     4741 aaaggcatta caataaagta cattacccac tatgcaggaa ggccatatcc ttccactttt
     4801 tctgagggca ttatttacaa atcaccgtta gaaataaatt caacaaaact ggttgctgca
     4861 atcggaatgt taggaaataa agctattagt aacccggttt atggaacatt tcgtttcaac
     4921 aaagccacgg gtaaaaaaat aacgctgacc gctcccccgt caaagagtta tcccggtgac
     4981 ggcgctttta ctttagtaaa tggagtacag aatgaaaaag gtttggctaa acgaaatgaa
     5041 ttcttagggt tcagcggtgc cgattgcgaa gcgataattg atttgggtaa ggatgagaaa
     5101 attgcgatgg taacagttca tgcactccat cagaccggca gctggatatg gagaccgtta
     5161 tccgctgaag tgcatacctc taccgatgga tctaagtacg agtttgcggg ccttacagat
     5221 gattttaatg tcactattaa aggactggaa aaagggaaaa tgaaaatcag ttttgataaa
     5281 aaagaagcaa gatttgtaaa aatcattgtc aaatgctggg aagaaatacc tgccggggaa
     5341 cccggtgctg gaaaaaaacc atggttattc attgatgaaa tcgaaataaa ttaacgcaaa
     5401 atgctgcaca aacctgcata aaccttcaat tttaatggct acctttacca aacaaaccaa
     5461 caatatggcg aaaagggcta atgcaattga tctttccgac agttttgaga agatacccgt
     5521 taaaatattt ccatcttcca aagaagggtc tgtatttgtt gcccggcaga tagctgattt
     5581 cattcgggaa aannnnnnnn nnnnnnnnnn nnnnnnnaaa gcaaaagaag aaagagaaat
     5641 gtgttttagg tcttgccact ggctccactc ctaaatcttt gtatgccgag ttggttcggc
     5701 tgcaccggga agaaaaactg agtttcaaaa acgtcatcac ctttaacctg gatgaatatt
     5761 acccgataga taataatgca ttgcagagtt acaaccggtt catgaaaaac cagttgttcg
     5821 atcatattga tattgatccc aaaaatatcc acataccaaa cggagagata aaaaaagaag
     5881 atattaaaga acattgccgg caatacgaga aaatgatcaa agatgccggt ggaattgaat
     5941 tacagatttt aggaattggt aataacggtc acattggatt caacgaaccg ggctctggta
     6001 tttattctaa aacaagattg attacgctgg acaattctac ccgtattgcc aatgcctatg
     6061 aatttgctaa tatttcacag gtaccaaggc tggccattac tatgggcatc agcaccattt
     6121 tacaatccaa aaaaattatt ttaatggctt ggggcagcgc caaagctccg gttattaaaa
     6181 aagcggtgga agatgatgat accgaacagg tgccggcttc tttgctgcag aaccacgatg
     6241 atgtgacttt cgtagtagat gaatcagccg ctgcagaact aacccgtaat aaatcaccct
     6301 ggctgaccgg cgattgtgaa tggacaccaa aaatgattaa aaaagctgtg gtgaacatgg
     6361 cactgaaatt aaaaaagcct gtcttaagcc tcaccaacag cgattataat gaatatggcc
     6421 ttagcgatct gctggtagaa aaaggagacg catatgaaat aaatcttcag gtttactata
     6481 tgctgcgtga ctcaattacc ggatggccgg gtggcaaacc ggattcgcat atacccaacc
     6541 acccggaaag atcggctcct tatcccaaaa gagtagttat cttctcccct cacccggatg
     6601 atgatatcat cagcatgggc ggtacttttc agcgattaca cgaccaggga catgatgtac
     6661 atgttgccta tcagacaagc ggcaacattg cagtcactga cgaatttgta acaagatttc
     6721 tggattttgc ggtaggcttt gaagagatag tcggtattga cactaaaaaa tccggaaaga
     6781 tactggatga tgcaagaaga tttattgccc gtaaaaaatc aaaccaggta gataccccgg
     6841 caatccgttc catcaaaggg ctgatccgtc gttgcgaggc aaaagccacc tgccgctatg
     6901 tgggcatcaa agaagaaaat attcattttc aaaacctgcc cttctacgaa acgggaacaa
     6961 ttgagaaaaa accaatggga gaaaaggatg taaaactcac catggaatta ttgcgcaaaa
     7021 taaagccaca gcaggtgtat tgcgccggcg actttgctga cccgcatggc acacatatcg
     7081 tttgtttcaa cgtggtagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nattatgaat ggataaatga ttgctggcta tggttataca aaggtgcatg gcaggaatgg
     7201 aatattgaag aaattgaaat ggctatccca atgagcccgg accaggtatt gaaaaagcga
     7261 ttcggtattt tcattcacca gtcacaaaaa gacatggttc ctttccaggg cagcgattca
     7321 agggagttct ggcaaagagc tgaagaaaga aatgcaggaa ccgccaatct ttatgctgac
     7381 cttggactaa cacattatgc agctatggaa gcatttgtca ggtggcaata ctgatttgtt
     7441 cattgtttct ccctaaattt attgttacaa aaacttttaa actatgtcga tattcaaatc
     7501 gagtaatcct gtaatgcagg aaaaaactta ccagggtaca attctggaag gcatcagtac
     7561 aggcgaagaa atgaccctta aaggaacgat gaataagttt ggggtgatga tcatgctgat
     7621 gattggctct acccttttcg catggagcca gttttacaaa ggcagtgatc cgaannnnnn
     7681 nnnnncattg atgatgattg gtgttttcgg tggtttagca cttgcactgg taatggcttt
     7741 taaaatgaaa tgggccagtt ttctagcacc tgcttatgca atattagaag gattgtttgt
     7801 aggatcaatt tctgccatat atgattatgc ttatccgggg ttgcccatgc aggcggttgc
     7861 gcttacctta ttggtaaccc tgatcatgtt cctgatttac cgttacaaga ttataaaagt
     7921 caccagcaaa ctaagaacag ttattgtagt ggccacctca gcagttgcaa tattttacct
     7981 tatacagtgg cttacttatg caataggcgg tactgccatt gcttataatt tcaccaactc
     8041 tgccacaccc annnnnnnnn ngttgtactt gcttcattta acctgctgct gaattttgat
     8101 accgttgaaa gaggggtaga aatgagggca ccaaaataca tggaatggta cagtgctttc
     8161 ggcctgatgg ttactgtggt ttggctatac ctggaaatac tgcgcttatt gtcaaaattc
     8221 caaagaaact aattctaact acctattata aaaaagcctc tcatatgaga ggtnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgagccat cgtggaagga
     8461 agtactaaag gatgagtttt caaaacccta ttttcagcag gtagtactgc atctaaaaac
     8521 ggaaaaatca cagggaaaag tcatttatcc gcccggtcct tatattttca acgctttcaa
     8581 cataacgcct gttgatcata taaaggtggt aatactcgga caggaccctt atcatgggcc
     8641 cggtcaggct catgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg cacgacgata tcggtgttga aatacccaac
     8761 catggcaatc ttacacattg ggcagaacaa ggagtctttc tactgaatgc ttcgcttaca
     8821 gtaagggccg gcgaacctat gagccatgct aaaatcgggt gggctacttt taccgataca
     8881 gtgatccgca ggatttcgga tttaaaagca catgtagttt ttatgctttg gggaaaattt
     8941 gcgcaggaaa aaaaaatatt gattgacgaa acaaaacacc tggtgttgaa agctgctcat
     9001 ccatcgcctc tttctgctca cgcaggcttc cttggaagca ggcacttttc caaagccaat
     9061 gaatggctca tcagcaaggg aattgatcct gtagactgga aattataaat caaactcaga
     9121 cagtatgaaa ctgctaaaag atatatttgg aaggttttgg gcattctggg gcattatcag
     9181 cttcatcggt actttcctgg tcatctatat accctcaatg atcacctggt tgctccctga
     9241 gcccaaagga caaagaatat tcatagggat agcacggatt tggatgaata tatggctaag
     9301 attggtgggc tgccctgtaa aaataaaagg ccgtcaaaat tttcaaagag gcaaaagtta
     9361 tattgttacc tgtaatcaca attcattgat ggatgttccg ctttcgtctc catttattcc
     9421 cggggccaat aaaacaattg ctaaatcatc gtttgccaaa gtgccgctgt ttggttttta
     9481 ttatatgaaa ggcgcggttc tggttgacag aaaaagcgaa aaaagccgca aagaaagttt
     9541 tgagaaaatg aaactggtat taaagaaagg gatgcatatg tgtatttacc cggaagggac
     9601 ccgcaaccgt actaatgagc cattaaaaaa attttatgat ggggctttca aactggcaac
     9661 ggatacaggt acagacatca tcccggccat aattctaaat actagaaagg ctgtgccggt
     9721 gcacaaaagt ttttattttc tgccacacaa actggaaatg cattttctgc cacctgtatc
     9781 agttgagaac aaagaaactg aagaacttcg tgaagaagtt tttgtaatca tgaaaaaata
     9841 ttacctggag catcagcatt agaaattcgt aaagaacctg ctcctgttga tacggaggtc
     9901 ctgcaacact gcagctttag gactaataga gaagctaaaa gaacgataaa gaccgattgg
     9961 cgtcacgttg attgacattt gccaacagtg catctccctg ttaatactca tagaaaaggt
    10021 ttgcatttct ttagtatcaa aatcataatt gccctgcgca gaaaaattcc attttggggt
    10081 aagattaaag cttccgttga agttcaaacc ggaagaaaag tctttctcaa aattgaccag
    10141 gtcggcgctc agcctggtgg tgaatgaaat agaataaccc aatgtaacct gccagggaat
    10201 attaaaatca acaaaatcac caggattttg ctgcatataa tccaataatc ttctctggtc
    10261 gccggcaagc gaaggatcgc ttaataattc ctcttgttgc ttttttcgca actcatcttt
    10321 tttaggatct tttggtttac tcttgaaact ggtgtttaca gacacacttc cataggtaag
    10381 cctgcccaat ttgaacttgc ccccctgcca ggcatacttg tttatatttc gcccatattt
    10441 gtccgtttgg taaggatcca atacggtctg agcattgatt tgtatcttat caaataaagt
    10501 tgaccgcagg taaatattaa aggcatccag ctttaaagaa tcacggagaa agttataacc
    10561 ctgttgaaaa accataacca tcgatcaact tcactttctt gattgcattt tcaccggtat
    10621 ccttttttga acgcattttc atttcgaggt tattatccaa ttgaaagccg ataccgcctg
    10681 tttttccttc accataacca gcatacaggg ctccttcaaa aacactaaaa cgctgtgtta
    10741 ctcctgttgc aaccgtatct gtgtaataaa atttcctcga cagatcaggc ttatagttca
    10801 gactgaaagt aggtcttatc acatggcgaa tggctacgat cctcgacttt ttgaactggt
    10861 aggttccaaa tatggcagta ttaaagctca acgaaaactg agcctgatga tccataaaga
    10921 accccttggt aatggatgta tccacttttt gtgtagctga attccatctc cgcctgaatt
    10981 tttgtgcaat ccaaacctga gagtaagaaa atcccggcga aacaattact gcaccaccta
    11041 atatgggggg caatgccagt gtaaccggga aactatgctg tgcaccccac tgtaatgtat
    11101 caatcaggtt tttcaactta aatgcagtat cataaaaaga aacctggttt ctgaagttac
    11161 cgttataagc aatacctaat ttttcatacc attttgcagt accggttgca tttttctttt
    11221 gtaatggata taatgtattt acggcaaatc ctgcatccgg caaacttatg ttgattagtc
    11281 ttgtcctgtt gttctggttg tgattggcac ttattgtcag gttataaggt tttcccgccc
    11341 atgatttgga ataggcaatg gatgaactca tctggttttg aaagttcagc tgcacattgt
    11401 ttggcaccaa ttgattgaat ttagtagaac cggcattcac actggcagag aaagttgttc
    11461 cgggacgggc acggctgtca accgagtgag tccagttaac catatagctt ttattcttta
    11521 aatagtcagg gtctcctttg aagttaaatc gggttgattg tacactaaga ttgaaagctc
    11581 cgttatagcg gtatcttttc ctgtaagagg gatttacatt ggctgaccag ccaccatatg
    11641 agtaaatgtt tccgtatact tttacatccc agtaatcgct aatcactttg taataaccta
    11701 atccttctaa tcccaaacca aattgttcgt tagtcgcaaa tcttggcggc aacagacccg
    11761 aatgtcttcc ctggcttaaa ggataaaaac caaatggcag gtaaatagga accggtacac
    11821 cttcaaattc aggatgagcc gggccagata ccgccagctt ttgattaatc actttcatct
    11881 ggggtgttac aaaggcaaaa tgcggatcat cgagcaagca ggtagtgaac cttgctcttt
    11941 tgataaaggt agtgttttca ctcactttct ttgccacttc accaataact aagaattccc
    12001 cttgttgagt gtaagtattt tttgtcaatc ctacctgtgt tttgaagttg tagcggatag
    12061 tatcgctggt aaactcactt tctccgcttt tgaaatgggc ggtttctgtt acagtacctg
    12121 tactgtcttt agcattcacg gctgtaacaa tctgtgtaac ctggtccact tccactttgg
    12181 gtgctgttaa agtgatatcc ttgtattccg tttttgtctt accatataga atgatttttt
    12241 tatcctgaat caaaacaaca gcagaatctg ccgcttcata cttcagggga gcttccagtg
    12301 aatctttaga aagtttaaga gaaaatgtat ctgtctttgg cttcaggcta gtatctatta
    12361 ccggtaattt ggttgtgtcc gtaactaatg ataaaatttt acggccggag tctgaaggat
    12421 ttacaggtct tgttgtgtct gttaaaggct tgtaaaagta tgacgaagaa tccgcataag
    12481 ccattgtttt ccacgtttga gtgaaaaaca taatagcagt taatatcccg gttaaaaaat
    12541 attttgacct aaatttgtac gaatgctcca tcgcaaggaa gcaaatgtaa atcattatcc
    12601 ataggacttt gatacttaga cctaaacaat ttgtgaatat tgtggaaaaa ctcaaaccga
    12661 aaaaccatga ttaaacgaac cctggtattt tttctcttta tttcatttgg tgtaaccgta
    12721 ttctcctttt ctgagaagga agaaaaaaag aaaggcaagc aaaaggtgat caatacgatc
    12781 gtaatcgatc ccggtcatgg cggtagtgat gccggagcac atggcgttta ttcttatgaa
    12841 aaagatattt gccttgatat ctccctcaaa ctgggcaaaa tgatagaaaa ggaatttcca
    12901 gacattaagt tgctgtatac ccgaactact gacacttatc ctgccctaca ctcccgggca
    12961 aattttgcca atgaaaacaa aggtgacctg tttctatgca tccatgttaa ctcggctccg
    13021 gctaaaagaa tgtcagaaat taccggctat aaaacagtta cttattatac aggcaaggga
    13081 aaaaatcgta aaaagaaaac aaaacaggtt cctcaatacc ggtattatac agtgccaagt
    13141 aatgcaaagg gtactgaaac ctatatctgg ggagcacata aatcagaaga aaaagaactg
    13201 gcggtgaggg agaactctta tatctacatg gaaaaagact ttgaaaaaaa ttatggcccc
    13261 aatttcgatc ctaactcccc ggaggcagta gcagtttcat tactgaaaac aaaacaatat
    13321 tttaaacgca gtgccacact tgcaggttat gtacaggatg aatttgccaa agttggccgt
    13381 gttgacagag atgtaaagca aagaggtgta ggtatatggg tacttcaggc tactgcaatg
    13441 ccgagtgtgc tggtggaaac aggttttatt tctaaccctg aagaagaaaa atacctgaac
    13501 agccaggaag gacaaaccga aattgcagat tgtatagtaa aggcgttgag gaaatatgta
    13561 aaatggctgg aagataagca ggaggacgat aaagagaaag atgaaaagaa agatgacggc
    13621 agcccgcaat ccgccggtct gaacagggac aacagggaat ttctggaaat ggttgacaaa
    13681 atggaaaaac aacgtatgag taagtaaaaa caaaaatacc cggcaaaaga ggatgaaaat
    13741 tcatcc
//
LOCUS       DAORLS010000047        13722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2392_length_13722_cov_1.937294, whole genome shotgun sequence.
ACCESSION   DAORLS010000047 DAORLS010000000
VERSION     DAORLS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2392_length_13722_cov_1.937294"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..>1426
                     /locus_tag="PK977_05485"
     CDS             102..>1426
                     /locus_tag="PK977_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17596.1"
                     /translation="MKKIVVLLTTLITGTAFAQNVGIGTDMPASKLTINGTDPDIGIM
                     NNGLAHGFIKASGVNLNIGTASDNPTGKLILGTKDNNHFNIDHLGRVSIGTNSSFDAS
                     LKLNGTSPIFGFLHEEVQKGFIRLGGDNFKFGTYPGNSGKIVFSPKNVDKIWIDEDGQ
                     MGIGTSTPVSALTINGTNPYIQIQNGASNKGFLQAVGNNLKIGTNSTNTTGSLVIQTK
                     LIDRMTIDENGLVGIGTTTPSSALTINGANPILQLRNNDVDKGFLQISGDDIKIGTNI
                     ANSAGNVIIRTNGVDRVNVDDGGTVTIGDATDGYLIINSDQPGMTLQKNNLTHANVYV
                     DDNTGDFIIEKTIQGNGRMVLRPAQGAGYNVYINENGQFNFGTGLHPPGYRFSIEGNV
                     IATGFTTQTVANWPDYVFEKNYRLRPLNEVKEFILANKHLPEIPSAAEI"
     assembly_gap    1427..1436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1589..>3490
                     /locus_tag="PK977_05490"
     CDS             1589..>3490
                     /locus_tag="PK977_05490"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF17597.1"
                     /translation="MKKTLAILSFYFISIFSMQAQPAFLHLSTAEGLSDNNVYTVARD
                     RNGILWIGTGEGLNSFDGNRITTYYKQQHPELPENSIARVLVDDENRVWVRTSGHHLT
                     VLDEKRRFHKILIGDTSDRSGIGNIFHTKKNGLIVLKGTKHYFQHKKTFSFEKKNLPF
                     ENKLAGVPGFTYFLDNDKVVYYRNSRLIVINYSTLEPELDYLFPGLTAATNINDDELL
                     LFTNKGDVFYRFSISQQKIIREYRNITDQYNMPVTGTLRIVTRIDSSRFAFTTYFSGL
                     YLLDLQKKSLEHWVHDPINPRSIGGNNTMNIKYDTTGYLFVTTQTSGLHFYNLKQKQA
                     FSKPYFMNDAKEIFDGYIQSLITDEDQNVWMGAQDRLIKWDRAADKTAFIPIKLADGT
                     NISGRETIRAVNTDEKGNLWVGTSLYGIYVFDKKMNTIARFTDVNTGLPSNFINALCR
                     DKYDNWWVGTPRGICIVNKTSLSITSLKNHQVLDTLSNINCTALWKDDNGDIWAGTTK
                     GVWHYSESTAKLVNYSSKDGLPNNTVEGINKDNLGNYYFTTLGGLSILTKDKKIVSYT
                     RSNGLRNDRCEGLLKDEKGFMWIGNLNCIMRFDPVSKTFAVYEEGYGFSHGGFRMRCA
                     HITPKGEMFW"
     assembly_gap    3491..3588
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3589..>3885
                     /locus_tag="PK977_05495"
     CDS             <3589..>3885
                     /locus_tag="PK977_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF17598.1"
                     /translation="HFTAKDEISFPYNTSSFIFNFSSGELSGDKKNQFLCRLSGFDNN
                     WVQPSLTGQAVYSKLPPGRYSFEIKASRDGINWYNASYPVEIIITTMVASMRTV"
     assembly_gap    3886..3895
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3896..>4185
                     /locus_tag="PK977_05500"
     CDS             <3896..>4185
                     /locus_tag="PK977_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17599.1"
                     /translation="WQQTWFRLLCILLSAAILVATYYFYKKRKSTKEINKTIEYFANS
                     SYEHSSVEDILWDISRNCISRLGFEDCVIYLADEEKKILVQKAAYGPKSPKP"
     assembly_gap    4186..4270
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4271..5184
                     /locus_tag="PK977_05505"
     CDS             <4271..5184
                     /locus_tag="PK977_05505"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF17600.1"
                     /translation="DTTKDSRYIVDDENRLSEITVPIIHEEKVIGIIDSEHSKKHFFT
                     KQHLQALQTIAALCSTKISRAMAMDAMKKSKLELMELNVKMAESKFLNLRLQMNPHFL
                     FNSLSSIQHLIVSQQATKAYKYLTVFSNFLRSLLNYADKNFIPLDAELKILQMYIELE
                     SLRFDQSFSYEIKADESLSNDEVLVPSLMVQPFAENAIWHGLLHKEGDKKLSIRFSNN
                     AEDYLTCIIEDNGIGRQKAESIQKGKITSMVHESKGIGIIRERLELLQQKTGKPASLK
                     IEDIYSNNEATGTKVIITIPYYNPEES"
     gene            5181..5927
                     /locus_tag="PK977_05510"
     CDS             5181..5927
                     /locus_tag="PK977_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF17601.1"
                     /translation="MIKAMITDDEQSSIDLLQWLIGQYCPDITAIKTARSVQEAIPLI
                     QSFKPDILFLDIQMPHQSGFDLLTTVDNWNFEVIFTTAYNEFAIQAIRFSALDYLLKP
                     VDETELKKAVERYKAKRIYAPAGQLLFRNFIQNISQGNKDKFKLALADATEVKYVQLD
                     EIIRLQAESNYTHIFLSGNKVFVSAKTLKEYDEILQGHKFLRVHKSHMVNPAHIDAYD
                     KQGTLVMSDGSRVEVARRKKEFLQQSLAGK"
     gene            complement(5931..6353)
                     /locus_tag="PK977_05515"
     CDS             complement(5931..6353)
                     /locus_tag="PK977_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17602.1"
                     /translation="MERQLNTGYAGRIQLRQLVNDLLNNSLPTAIQNRSDVVNEVGEG
                     VVLQGIDTKMVKLLNEVIFTVLNNSNRGDIHIRSERYKDHVELRIIDKSNYNGYSLSF
                     SIGAIIPDASRIGADIMLMNHQQREATVVFSIPGNFAA"
     gene            complement(6591..7082)
                     /locus_tag="PK977_05520"
     CDS             complement(6591..7082)
                     /locus_tag="PK977_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17603.1"
                     /translation="MKYILSVLLLVSIAAAGQDCKLNRETDPFTKEVKLSTGFIFVEG
                     GSVTVDADSKEIVVLFSIEGADKCFDNNSTASIVFEGIKSKSASRNGGTMNCEGLFQF
                     VFRNSASTTTLLQKLMTQKLASIVFTGNNKKESTLTVGPQEQEAIMRLTSCLVNEAKT
                     LIK"
     gene            complement(7233..7778)
                     /locus_tag="PK977_05525"
     CDS             complement(7233..7778)
                     /locus_tag="PK977_05525"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF17604.1"
                     /translation="MHKVFFALLASSLLSSVVAGQTTPVTTDAILQEAKQLASKENKN
                     ILILFTASWCGWCHKMDKSIDDPSCKSFFEENYVIRHIVVDETRDKKHLENPGGGEMK
                     KQYHGEGLGIPFWLVFDKEGQLLADSKIRKEGEGPEKGENTGCPATAEEVDHFIAVLK
                     KTSRMTDAQLEIVKKRFRRNE"
     gene            complement(7786..8103)
                     /locus_tag="PK977_05530"
     CDS             complement(7786..8103)
                     /locus_tag="PK977_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HRF17605.1"
                     /translation="MLITTTSAVDGRPVQEYLGVINAQSIIGANIFKDIFAGLRDVFG
                     GRSKTYEKVLEQAKEDALRELSQKAQAMGANGILGVDLDFETIGSGGSMLMVIATGTA
                     VRL"
     assembly_gap    8216..8243
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8244..9194)
                     /locus_tag="PK977_05535"
     CDS             complement(<8244..9194)
                     /locus_tag="PK977_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF17606.1"
                     /translation="MKSYFGSFYFSHGTEAAEATVLVFDKNLSIGYRNPDGTTSTINW
                     LLKDVEASFEFGRQQTRLRHAGGGGELWIEGNDAANFVKERQEEQKKAWYKKSSGKEC
                     IRNSLLLLGLAGLLVLLYLLIVPWLSQKLASKISPNTEQQIGDAVYDAMGLRSMEDST
                     ATLLLNDFFAAMEVPTAYQIRISVVNDNVVNAFALPGGRIVVYKALLQQINSYPELAA
                     LLAHEFTHVNNKHSTKRIFRTMGSKVFLGLLFGRFGKVTSVVVDHADDLKSLHYSRSL
                     EKEADMDGLQLLTQRKIDPAGFSELFTHLKQAASGSVMPEF"
     assembly_gap    9271..9296
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9363..>9861
                     /locus_tag="PK977_05540"
     CDS             9363..>9861
                     /locus_tag="PK977_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17607.1"
                     /translation="MVQAYNFLASWQLFPEKGTYENGDRPKSGIYKIEAVPDTKELAI
                     YHNWVTLENQAFAANYKILVDGGLNDFNDHLLADKVQAVIPDGITFEIHFYKKGEVVL
                     HVVHEIMPNGYLKITQQGQKEDGTAYTNTEVYHKQLSVLPYSASVAGAVIKPNEEGII
                     KHKALT"
     assembly_gap    9862..9887
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9888..10152
                     /locus_tag="PK977_05545"
     CDS             <9888..10152
                     /locus_tag="PK977_05545"
                     /inference="COORDINATES: protein motif:HMM:NF021983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HRF17608.1"
                     /translation="LDQIRKQIELLALQAHEIQKRKELSMMIYNARLSFKPNIGQIYY
                     LYEKQDDSFMLSLVSPKEWGNSGPFKKFVAAVQLLADHTWKEL"
     assembly_gap    10228..10297
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10298..10848)
                     /locus_tag="PK977_05550"
     CDS             complement(<10298..10848)
                     /locus_tag="PK977_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17609.1"
                     /translation="MKRYFLTLILAIGFMIRPASVQAQSYDLIEDALEQTMQALENLL
                     GDMEGFEEGVAPAFEQFRDWMETARSGLDLYNSMQALDNNECVPDFTTPAEAMMPSGC
                     RSNEECAGCYEEPVRRLNVVRKSLARLRCIYTNTKRFTDAALSFGDNVSGIHGASGLA
                     WQSERRGIEQSWRNFEQTYDRKYT"
     gene            complement(10880..11485)
                     /locus_tag="PK977_05555"
     CDS             complement(10880..11485)
                     /locus_tag="PK977_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17610.1"
                     /translation="MRKNCFLLLFLFIAASVTAQPFNLDTSINPVEIKLRPFKPTHKD
                     SALQKGRMNIIRVTQVKDTLYYFVQGASIYSPVFVGVTSLDPANKIKVQLHKMNWQNA
                     SRSGTTDDKGQWSEKFKTENDFGIMVIPGSKPAKYSILVWVGDEPKMELPSVFSGDAK
                     EDKAADKGGKGGSGNTLLYVIIGVLAVAVGFLFFKMKNKKA"
     gene            11587..>11737
                     /locus_tag="PK977_05560"
     CDS             11587..>11737
                     /locus_tag="PK977_05560"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRF17611.1"
                     /translation="MTKLSVNINKFATLRNSRGGNNPDVIKAAIDAQRFGADGVTVHP
                     RPDERH"
     assembly_gap    11738..11747
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11748..12312
                     /locus_tag="PK977_05565"
     CDS             <11748..12312
                     /locus_tag="PK977_05565"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRF17612.1"
                     /translation="YDDVRQIKKIITTEYNIEGNCREQKFIDLVLETKPTQVTLVPDE
                     LGQLTSDHGWDTITHKDYLKETISIFKNAGIRVSIFVDPVLAMLEGAAATGTDRIELY
                     TEGYAKEYAAGNKQTAVRPYVEAAKKAKELGLGINAGHDLDLHNLQYLKQHIPFIDEV
                     SIGHALICDALYLGFENAIQLYKRQLQ"
     gene            12379..>12604
                     /locus_tag="PK977_05570"
     CDS             12379..>12604
                     /locus_tag="PK977_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17613.1"
                     /translation="MCHTRRHPKMKEIILTISLLFTISAFGQTRSEISNSKLYVRATY
                     FDKKNKFIGLQTRADLSDTIINSIKYKKIRT"
     assembly_gap    12605..12687
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12778..>13722
                     /locus_tag="PK977_05575"
     CDS             12778..>13722
                     /locus_tag="PK977_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17614.1"
                     /translation="MEFIDTRNSFPRDSLIPTEKTPRKYYQKDNSEIYLVIIPDLKTL
                     AVSSNGQFYAKQLFGDNYNDISNGLENNFQASTKFNIQKGDEIQLFYRRKWYNDTTNV
                     AEYEDKQFKNIKFLGDTVLNNTQALKLEIEGYNYLSGTKDNPEELLVSLTDSGYNVGY
                     QFVPFKNYKTELKIVDNNGNKEFFLQGVTFDTVGNNVYPKIVQAKSNDPYRYFILPFF
                     PMPFIEFGNVQGIITYSKIKGIEKGTKRERTYITSENNIRDIISKSKNEVEVSIYFID
                     KADIEIEIQDFNTDKTVEQLKSSAKQGLNTFIVRADKLE"
BASE COUNT         4187 a         2862 c         2634 g         3593 t
ORIGIN      
        1 ttttgcaaaa accggaaaat aggaaactct taccgtaaac taaccctgat tgacattcaa
       61 cataaccact atgttacttt catggcataa actattaaac catgaaaaaa attgtcgtat
      121 tactgacaac cttaatcacc ggaacagcct ttgcacaaaa tgtaggaatc ggtactgata
      181 tgcctgcctc aaaactcacc atcaacggca ctgatccgga catcgggatt atgaacaatg
      241 gccttgcaca tggcttcatc aaggcaagtg gtgttaattt aaatatagga accgccagcg
      301 acaacccaac aggtaaactg atattgggaa caaaggataa taaccacttt aacattgatc
      361 acctgggaag agtatccata ggtaccaatt caagtttcga cgcatcgctg aagttaaatg
      421 gaacctctcc cattttcgga tttttgcatg aagaagtaca aaaaggattt atccgccttg
      481 gcggtgataa tttcaagttc ggtacctacc ctgggaattc gggaaagata gtattctccc
      541 caaaaaatgt cgataagatc tggattgatg aggatggtca aatgggtatt ggaacttcta
      601 cgccagtgtc agcactcact atcaacggta caaacccata catacagata cagaacgggg
      661 catccaacaa aggcttttta caagcagttg gcaataacct gaaaattgga accaactcta
      721 ccaatacaac aggtagcctc gtaatacaaa ctaaattgat tgacaggatg acaattgatg
      781 agaacggtct tgttggaatt ggtactacca ctccttcttc cgccctgact atcaatggag
      841 caaacccgat ccttcaatta agaaacaacg atgtagataa aggatttctt caaatttcag
      901 gagatgatat taagatagga accaacattg caaatagcgc aggcaacgtt attatacgta
      961 ccaacggagt tgaccgggtt aatgttgatg atggtggtac agttacaatt ggtgatgcca
     1021 ccgacggtta tcttattatt aattctgacc agccaggcat gacactgcaa aaaaataatc
     1081 tcactcatgc caatgtttat gtagatgata ataccggtga ctttattata gaaaaaacaa
     1141 tacaaggcaa tggccgtatg gtacttcgtc cggctcaggg tgcgggttat aatgtatata
     1201 taaatgagaa tggtcagttt aattttggaa caggattaca tcctcccggc tatcggttca
     1261 gtatagaagg taatgtaatt gctaccgggt tcaccacaca aacagtcgct aactggccgg
     1321 attatgtatt tgaaaaaaac tacaggttga ggccattaaa tgaagttaaa gagtttatac
     1381 tggccaataa gcatttaccg gaaatcccgt ctgcagccga aattgannnn nnnnnngttt
     1441 agaccttaca gaaataagca agaaactaac ggagaaagta gaagaactta cattgtatat
     1501 ccttcagcag caggaacaaa ttgacgaact gaaaaaactg gttcagtcac aaaagaaaaa
     1561 tgactaattt atagcttgct taaatgcctt gaaaaagaca ctggccatac tcagctttta
     1621 tttcatcagc atattctcca tgcaggcgca gcctgcattt cttcatttga gtacagcaga
     1681 aggtctgagc gataataatg tgtataccgt tgcacgggac aggaacggta tcctctggat
     1741 tggaaccggt gaagggttga acagctttga cggcaacagg attacgacct actataaaca
     1801 gcaacaccct gaacttcctg aaaatagcat cgcccgggtt ctcgttgatg atgagaacag
     1861 ggtttgggta agaacatccg gccaccacct tactgtactt gacgaaaaaa gacggtttca
     1921 caaaatactt attggagata ccagtgaccg cagtggcatt ggtaatattt ttcatacaaa
     1981 aaaaaatggt ctcattgtat taaaaggcac aaagcattat tttcaacaca aaaagacatt
     2041 ttcatttgaa aagaaaaatc ttccgtttga aaacaagctt gcaggagttc caggctttac
     2101 ctatttcctg gataatgaca aagttgtcta ttaccggaat tcgaggctca ttgttattaa
     2161 ttattctaca cttgaacctg aactggatta tctcttccct gggctaacag ccgccactaa
     2221 tattaatgat gatgaattac tgcttttcac taataaaggc gatgtctttt accggttcag
     2281 tatcagccaa cagaaaataa taagagaata taggaatatt actgaccagt ataatatgcc
     2341 ggttaccgga acccttcgca ttgtaacccg tatcgacagc agcaggtttg ctttcacaac
     2401 ctacttctcc gggctctacc tgttagacct tcaaaaaaag tcgctggaac attgggtaca
     2461 tgaccctatt aacccacgca gtattggcgg caacaataca atgaatataa aatatgacac
     2521 aaccggttac ctttttgtaa ctactcaaac ctcaggtctt catttttaca acctgaaaca
     2581 aaaacaggct ttctcgaagc cctattttat gaatgatgca aaagagatct ttgatggcta
     2641 tatacaatct ttaattacag atgaagatca aaatgtatgg atgggcgccc aggatcgttt
     2701 gataaagtgg gaccgagcag ccgataaaac agcattcata cctataaagc ttgctgatgg
     2761 caccaatata agcggcagag aaacaattag ggctgttaat acagatgaaa aggggaactt
     2821 atgggtgggc acctcccttt atggtattta tgtatttgac aaaaaaatga ataccatcgc
     2881 caggtttacc gatgtaaata ctggtttacc ttccaacttt attaatgcac tatgccggga
     2941 taaatatgat aactggtggg tgggcacacc aagaggaatt tgcatagtga ataaaaccag
     3001 cttatccata acatcgttaa aaaatcacca ggtactggat acgctaagca atataaactg
     3061 tactgccctt tggaaagatg ataacgggga tatatgggct ggcactacca aaggagtctg
     3121 gcactacagc gaatccaccg ctaaactggt aaattatagt tccaaagacg ggcttccaaa
     3181 taacacagtg gagggaatca ataaagacaa cctgggtaat tattatttta ctacgcttgg
     3241 tggcttgtca atattaacaa aggataaaaa aattgttagc tatacacgca gcaatggctt
     3301 gcgaaatgac cgttgtgaag gattgctaaa agatgaaaaa ggttttatgt ggatcggcaa
     3361 cctcaactgt attatgcgat ttgatcctgt cagtaaaact tttgctgttt atgaagaagg
     3421 ttatggattc agccacggag gattcagaat gcgctgcgcc cacataaccc caaaaggtga
     3481 aatgttctgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc atttcactgc
     3601 aaaagatgaa atcagtttcc cgtacaatac atcatctttc attttcaact tttcatcagg
     3661 cgaattaagc ggtgataaaa aaaatcaatt tctctgcagg ctttcaggtt ttgataataa
     3721 ctgggtacag ccatccctga cgggacaggc cgtttacagc aaactccctc cgggcaggta
     3781 ttctttcgaa attaaagcat cccgtgacgg catcaactgg tataatgcat cttatcctgt
     3841 tgagattatt attacaacaa tggttgcttc tatgaggacc gtaatnnnnn nnnnnggtgg
     3901 cagcaaacat ggttcaggct tctttgcatt ttactctctg cagccatact ggtagccact
     3961 tactactttt ataaaaaaag aaaaagcaca aaggagatta acaaaacaat tgaatacttt
     4021 gccaactcgt cttacgaaca ctcttcagta gaagatatac tttgggatat ctcccgaaac
     4081 tgcatttcac gacttggatt tgaggattgt gtgatttacc tcgcagatga agaaaagaaa
     4141 atattggtgc aaaaagccgc ctacggtcct aaaagcccta aaccannnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn atgatactac caaagacagc cgttatatcg ttgacgatga aaaccgcctt
     4321 tccgaaatca ccgtcccaat cattcatgaa gaaaaagtga ttggcattat cgattcggag
     4381 catagtaaaa aacatttctt caccaaacaa catttacagg ccctgcaaac cattgcggcc
     4441 ctttgttcaa ctaaaatttc aagggccatg gcgatggatg ccatgaagaa gagcaaactg
     4501 gagttgatgg aactgaatgt aaaaatggct gaatcaaaat tccttaacct gcgattgcag
     4561 atgaacccgc atttcctgtt taattcatta agctccatcc agcacctgat cgtttctcaa
     4621 caggccacca aagcttataa atacctgacc gttttttcta actttttacg ctccctgttg
     4681 aattacgctg acaaaaactt cataccgctt gatgcagaac ttaaaatact ccagatgtat
     4741 attgagctgg aatcgcttcg cttcgaccag tcatttagtt atgaaataaa agcggatgaa
     4801 agtttaagca acgatgaagt actggtgccc tcgctgatgg tgcagccttt tgccgaaaac
     4861 gctatctggc atggcttact tcacaaggaa ggagataaaa aactatctat tcgcttttcc
     4921 aataatgctg aggattacct cacttgtatt attgaagaca atggcatagg ccgtcaaaaa
     4981 gctgaatcaa ttcaaaaagg caagatcacc tccatggtac atgagagcaa gggcattggt
     5041 atcatcaggg aaagactgga attactgcag caaaaaaccg gaaagccggc cagcctaaaa
     5101 atagaagaca tttattcaaa caacgaagcc accggcacca aagtgatcat aaccattcct
     5161 tattacaacc cagaagagtc atgataaaag caatgataac agatgatgaa caatcttcga
     5221 ttgacctgtt gcaatggctg atcgggcaat attgtcctga catcacagct attaaaacag
     5281 cccgaagcgt tcaggaagca atcccgctga tacagtcttt caaaccagat atactgtttt
     5341 tagatataca aatgccccat cagagcggct tcgacctgct gactacggtt gataactgga
     5401 attttgaagt aatattcacc acggcctaca atgagtttgc tatacaggct attcgcttca
     5461 gtgcacttga ttatttatta aaaccggttg atgaaactga attaaaaaaa gcagtagaaa
     5521 gatataaggc caagcggatc tatgcacctg ccgggcaact actgttccgg aatttcattc
     5581 agaatatttc acagggcaac aaggataaat ttaagctggc actggcagat gccacagaag
     5641 taaaatatgt acaactggac gagatcatcc gcctgcaggc agaaagcaat tacacccata
     5701 tttttctatc tggcaataaa gtatttgttt ctgccaaaac actgaaagag tatgatgaaa
     5761 ttttacaggg acataaattt cttcgggtgc ataaatctca catggtcaat ccggcacata
     5821 tagatgctta tgacaaacag ggcacattag tgatgagcga cggctcccgg gtagaagtag
     5881 cccggcgaaa aaaggagttt ttgcagcaat ccctggcagg caaataatac tcaggctgcg
     5941 aaattacccg gtatagagaa tacaacagtg gcttcccttt gttggtggtt cattagcatg
     6001 atatcagcgc cgatacggga tgcatccggt ataatggccc ctatgctgaa ggatagactg
     6061 tacccgttat agttactctt atcgatgata cgcaattcca catgatcctt gtatcgctca
     6121 gaacggatgt ggatatcacc cctgtttgaa ttatttaata cggtaaagat gacctcgttc
     6181 agcagcttga ccattttggt atcaattccc tgtagaacaa ctccctcacc aacttcattt
     6241 accacatcgc tgcggttctg aatcgcagtt gggagggagt tattcagcaa gtcgtttacc
     6301 agttgccgga gttgaatccg tccggcgtag ccagtgttta gttgtctttc cataaggttg
     6361 ggattttggg tcttttcaag gaacatggtc atttgatttt tcaaaggatg gattagtgat
     6421 tggacattct ggacgtttga acattatcaa aatcggaaca ctaagtaacg acaggaaaag
     6481 gccgctgcaa atagtattct tttggattgt tgtgtagtaa ccctgccaca agaaatgtaa
     6541 atcccaatag tgcaggtaag tgtttctgct gtaaggtcgt agaattcctt ctatttaata
     6601 agggtttttg cttcgttaac caggcagcta gttaagcgca taatggcctc ctgctcctga
     6661 ggacccacag ttagtgtcga ctctttttta ttgttgccgg taaacacaat ggaagccaat
     6721 ttttgggtca ttagtttctg taaaagtgta gtagtactgg ccgagtttct gaaaacaaac
     6781 tggaaaaggc cttcacagtt catcgtaccg ccattccggg aggccgattt ggatttgatg
     6841 ccttcgaaaa caatactggc ggtggaatta ttatcaaaac atttatcagc accttcaatg
     6901 gaaaaaagta caacgatctc tttgctgtca gcatcaaccg ttacggaacc accttcaaca
     6961 aaaataaagc cggtagatag cttcacttct ttggtgaaag ggtcggtctc cctgttcagc
     7021 ttacaatcct ggcctgcagc agctatagaa actaaaagta aaactgagag aatatatttc
     7081 atgagtatat ttttgtttta gaaatttgtc tcatgttatt aaccaaaata aatattcatt
     7141 aaaaaattag taagaaaatg aatattgtat tttggctcat ttcataaaat atatgatttc
     7201 ttacctgaag aagacaaatg gcctttaggt tgctactcgt ttctccggaa tcttttcttt
     7261 acaatttcca attgtgcgtc cgtcatccgg gaagttttct tcagcacggc aataaaatga
     7321 tctacctctt ccgcagtggc aggacaacca gtattctcac ccttttctgg tccctctcct
     7381 tccttacgaa tctttgaatc agccagtaat tgaccttctt tatcaaacac cagccagaaa
     7441 ggaattccca ggccttcccc atggtattgt ttcttcatct caccgccacc cggattttcc
     7501 aaatgcttct tatcccttgt ttcatccacc acaatgtggc ggataacgta attctcttca
     7561 aaaaaagatt tgcaggacgg atcatcaata cttttatcca ttttatgaca ccagccgcac
     7621 catgatgccg tgaaaaggat aagaatattc ttattctctt tcgaagcaag ttgtttagct
     7681 tcctgcaaaa tagcatcagt tgtaaccggt gttgtttgcc cagctacaac tgatgaaagc
     7741 aggcttgatg ccagtaaagc aaagaatact ttatgcatac actacttata aacgtacagc
     7801 cgtaccggtg gcaatcacca tcagcatact gccgccgctg ccgattgttt caaaatcaag
     7861 gtctacacct aaaatgccgt tggcgcccat agcctgcgct ttctgtgata actcccgtag
     7921 agcatcttcc ttagcctgct ccagcacttt ctcataggtt ttactacggc cgccaaatac
     7981 atctctcaat ccggcaaaga tgtccttaaa gatattggcg ccgataatac tttgcgcatt
     8041 gataacaccg agatactctt gtacaggtct gccgtctacg gcactggttg ttgtgatcag
     8101 catatgattg tggtttattt taattcttca aaaatagctt ttagccttac attttcactt
     8161 ccttccgcat tttttgacgc ttctttaata taagcgatgc gcttatcaat atccgnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnngaactca ggcattacgg aacctgaagc agcttgtttc
     8281 aggtgagtaa acaattcgga aaaacctgcc ggatcaattt ttcgctgtgt cagcagttgc
     8341 aggccatcca tatcggcttc cttctccagg ctgcgggaat aatgcaatga tttcaggtcg
     8401 tcagcatgat ccaccactac cgatgtcact ttaccaaacc ggccaaataa caaacctaag
     8461 aagacttttg agcccatggt acggaatatg cgtttggtgg aatgtttgtt gttgacatgg
     8521 gtaaactcat gcgccagcaa cgcagccagc tcggggtaag aattgatctg ctgcaataaa
     8581 gctttgtaca ccacgatccg tccgcccggc aaggcaaaag cattcaccac attgtcattc
     8641 accaccgata tcctgatttg ataagctgta ggaacttcca ttgcagcaaa aaaatcgttg
     8701 agcagcagcg tagcggtgga gtcttccata ctgcgcaatc ccatagcatc atacacggca
     8761 tcaccaatct gctgttcagt attaggtgaa atctttgaag ccagtttttg cgataaccag
     8821 ggaacaataa gcaggtaaag caatacaagt aaccctgcca gtcccagtaa caataaactg
     8881 tttcttatac actctttgcc gctgcttttt ttgtaccagg cctttttttg ttcctcctgt
     8941 ctttctttta caaaattggc cgcatcattt ccttcgatcc ataattcacc gccaccacca
     9001 gcatgccgca gccttgtttg ctgacggcca aactcaaaag aagcttctac atccttcagt
     9061 aaccagttga tggtagaagt agtaccgtca ggattgcgat aaccaatgct gaggtttttg
     9121 tcgaatacaa gcaccgttgc ttctgcagct tcagtaccat gtgaaaaata aaaggagcca
     9181 aagtagcttt tcattttaca tcttattgct ggcctgtaaa tatagttgat taaaaaagtt
     9241 cgtttgtgtt aaagtatccc ccaacaaagc nnnnnnnnnn nnnnnnnnnn nnnnnnaagt
     9301 gctcagaaaa caaaatcttt gcagcctgat tcagcctcgc atcaggcctc agctatcaaa
     9361 atatggtaca agcctataat tttcttgctt cctggcaact gtttcccgaa aaaggaacct
     9421 atgagaacgg agatcgtccc aaaagtggta tttataaaat tgaagccgta cccgatacca
     9481 aagaactggc catctatcac aactgggtta cattagaaaa ccaggccttc gccgccaatt
     9541 ataaaatact ggttgatggc ggactaaatg acttcaacga tcatctgctg gctgataaag
     9601 tacaggccgt catccccgac gggattactt ttgaaattca tttttacaaa aaaggagaag
     9661 tggtgctgca tgtggtgcat gagatcatgc ctaatggtta tttgaagatt acgcagcagg
     9721 gacaaaaaga agacggcacc gcttacacca atacagaggt ttatcacaaa caattgagtg
     9781 tgcttcccta ttcagcatcg gtagcaggtg cagtaataaa accgaacgaa gaaggcatta
     9841 tcaagcacaa ggcgcttact gnnnnnnnnn nnnnnnnnnn nnnnnnngct ggaccagatc
     9901 cgtaaacaaa ttgaactgct ggccttacag gcacatgaaa tacaaaagcg gaaagagctg
     9961 tctatgatga tctataatgc aagactttct ttcaaaccca atattggcca aatttattac
    10021 ctctatgaaa agcaggatga cagctttatg ctttcattgg tatcacccaa agaatgggga
    10081 aacagcggcc cttttaaaaa gtttgtagcc gccgtacagt tactggccga tcatacctgg
    10141 aaagagttat aactattcag accgtttgta tttctccttc atgatctcga ggtaaaggat
    10201 accaaaccgc tggtaccagt cccgcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta tattttctgt cgtaggtctg
    10321 ttcaaaattg cgccagctct gttctatacc tctccgctcc gattgccagg caaggcctga
    10381 tgcgccatgt atgccggata cattatcgcc gaaagagagg gctgcatctg taaaccgctt
    10441 ggtattggta tagatacaac gcaaccgggc tagactcttt cttacgacat tcagtcttct
    10501 taccggctct tcataacaac cggcacattc ctcattggaa cggcaaccac tgggcatcat
    10561 tgcttctgcc ggtgtagtaa aatctggcac acattcattg ttgtcaagtg cctgcatact
    10621 attgtacagg tcaagaccag aacgggcagt ttccatccag tcacgaaact gctcaaaggc
    10681 aggcgccacg ccttcttcaa aaccttccat atcccccagc aggttttcca aagcctgcat
    10741 ggtttgctcc agtgcatctt ctatcagatc gtaagactgt gcctgtaccg aagcaggacg
    10801 tatcataaat ccaatggcca atatgagtgt aagaaaataa cgtttcatat actaagtttt
    10861 ttttagctat tattgaatgt tatgcttttt tatttttcat cttgaaaaac aagaatccca
    10921 ctgccacagc cagtacgcca ataatcacat agagcagtgt attgccggat cctccttttc
    10981 cccctttatc tgcggctttg tcttctttgg catctccgct gaatacagat ggcaattcca
    11041 tcttgggttc atcgcccacc caaaccagta tgctgtactt ggcaggcttg ctgccgggaa
    11101 tgaccatgat gccaaaatca ttttcggttt taaatttctc actccactgc cctttgtcgt
    11161 ctgtggtgcc gctacggctg gcattctgcc agttcatttt atgcagctgc actttgatct
    11221 tgttggccgg atccagtgaa gtaacaccca caaacaccgg tgaatagata ctggccccct
    11281 gcacaaaata gtacagtgtg tcctttacct gcgttacccg tatgatattc attcggccct
    11341 tttgaagagc agaatctttg tgtgtaggtt tgaaaggccg gagtttgatc tccaccgggt
    11401 tgatggaagt atcaaggtta aatggctgtg ctgttactga tgcagcaata aaaaggaaca
    11461 gcagcagaaa acaatttttt cgcataaagt attaatgatt ttggataaat aaatgtaaca
    11521 aatcaatgcc agaatacaaa tcagtcattt aacacatctt attggacgga atagtaattt
    11581 tacacaatga cgaaactatc ggtcaatatc aacaagtttg caacacttcg caattcaagg
    11641 ggcggcaata accccgatgt aataaaagcg gccattgatg cccagcgttt cggcgctgat
    11701 ggggtaacgg tacacccccg ccctgatgaa cggcatannn nnnnnnncta cgatgatgtg
    11761 cggcagatta aaaagatcat cactacggag tataatattg aaggcaattg cagggagcaa
    11821 aaattcattg acctggtgct ggaaacaaaa cccacacagg taaccttagt gccggacgaa
    11881 ctcggccagc tcaccagcga ccatggatgg gacaccatta cacacaaaga ctatttaaaa
    11941 gaaactattt ccatttttaa aaatgcaggt atccgtgtca gcatatttgt tgacccggta
    12001 ttagcgatgt tagaaggcgc tgccgcaacg ggtacggata ggattgaact atacaccgaa
    12061 ggttacgcca aagaatatgc agccggcaat aaacaaaccg ccgtaagacc ctatgtggaa
    12121 gccgctaaaa aagcaaaaga actgggcctg ggtatcaatg ccgggcatga ccttgatctg
    12181 cacaacctgc aatacctgaa acagcatatt ccctttatcg atgaagtaag catcggccat
    12241 gcccttatct gtgatgcctt gtaccttggt tttgaaaatg cgatacaatt gtataaacgg
    12301 cagttgcaat aagctggcat aaattaacac tgattatcaa tagaaattta tgcctggagt
    12361 ggcgtatatt tatgtgttgt gtgccataca agacgacacc ctaaaatgaa agaaataata
    12421 ctgaccatct cactgctttt caccatttct gctttcggac agacacgaag cgaaatttct
    12481 aactcgaaac tttatgttcg agccacttac tttgataaga aaaataagtt catcggacta
    12541 caaacaagag ccgacttgtc tgacactatt ataaacagca ttaagtacaa aaaaatccga
    12601 accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnngac aataagctaa acacagttca taagataaac
    12721 tataagacaa cgaaagagca acaaggaata attttcggcc aaccaagaca agtttcattg
    12781 gaatttatcg acactcgaaa tagttttcca agggacagtt taattccaac agaaaaaaca
    12841 ccaagaaagt attaccagaa agacaattca gaaatttatc ttgtaattat tccagacctg
    12901 aagacacttg cagtttcaag taatggacaa ttctatgcga agcagctttt tggtgacaat
    12961 tataatgaca tctcaaacgg attggaaaac aattttcagg cttccacaaa gttcaacatc
    13021 caaaagggcg acgaaataca acttttttac agaagaaaat ggtataatga cacaacaaac
    13081 gttgctgaat acgaggacaa gcaatttaaa aacataaagt ttttgggcga cacagtttta
    13141 aataacaccc aagcattgaa gttggaaatt gaaggctaca attacttgag cggcacaaaa
    13201 gataatcccg aagagttatt ggtttcacta acagatagcg gatacaatgt aggttatcag
    13261 tttgtacctt tcaagaatta caaaaccgaa cttaaaattg ttgacaacaa cgggaacaag
    13321 gagttctttt tgcaaggagt aacattcgat acagttggca ataacgtcta tcccaaaata
    13381 gttcaggcaa aaagcaatga cccttacaga tattttatct tacccttctt tccaatgcca
    13441 tttatagaat ttggaaacgt ccaaggaata attacctact ccaaaatcaa aggaattgaa
    13501 aagggtacaa aaagggaacg aacctacatc acaagtgaga acaacattag agacatcatc
    13561 agcaagtcaa aaaacgaagt tgaagtaagc atctatttta ttgataaagc agacattgaa
    13621 atagaaattc aagatttcaa cactgacaaa acagttgaac agttaaaatc atcagcaaaa
    13681 cagggactga acaccttcat tgttcgtgca gacaaacttg aa
//
LOCUS       DAORLS010000048        13617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2415_length_13617_cov_2.579708, whole genome shotgun sequence.
ACCESSION   DAORLS010000048 DAORLS010000000
VERSION     DAORLS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13617
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2415_length_13617_cov_2.579708"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>287
                     /locus_tag="PK977_05580"
     CDS             <1..>287
                     /locus_tag="PK977_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17615.1"
                     /translation="AQEDTTQPPATDTTAAPAEEPPAEEEEPSLISPSIDFTSVQKGD
                     NSIDLKVGMRAKVKGQFINLYKMKVSFFQVVNEEDIPLGFKITDGNGKDIF"
     assembly_gap    288..299
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <300..891
                     /locus_tag="PK977_05585"
     CDS             <300..891
                     /locus_tag="PK977_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17616.1"
                     /translation="DSLKSDAEGKLNFKVVFAGNKAMESAEEYVSFRRGRLEIVPLKE
                     DSLLTVQIKLIDIGTGEETPVKETAVGVFVKRMFLPMKVGEATTDENGEGSVEFPNNL
                     PGDAKGNLTLLARLDENETYGNMAAAVVQPWGVPVSDKPEAQPRSLWSSHPPLWMLIT
                     FIVLMLAVWGHYIVIVYELFRLRKEQPNAPTHATNS"
     assembly_gap    998..1031
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1032..1327
                     /locus_tag="PK977_05590"
     CDS             <1032..1327
                     /locus_tag="PK977_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF17617.1"
                     /translation="NKVNPIKGDAASLEKGKALWSQHCKSCHGSKGKGDGPKASQLDT
                     EPGDFTKASFQSQTDGALYYKTNAGRDDMPSYKTKIPDANDNWALVNYMRTFK"
     gene            1536..3392
                     /locus_tag="PK977_05595"
     CDS             1536..3392
                     /locus_tag="PK977_05595"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRF17618.1"
                     /translation="MSTQTNFFAMETAPARAELETWVKKVAEFCEADAVHWCDGSKEE
                     YDRMCQLLVDKGTFIPLNPEKRPNSFACFSDPSDVARVEDKTFICSLRKEDAGPTNNW
                     MDPNEMKKILEGLMKGSMKGRTLYVIPFCMGPLGSAYSRYGVQITDSEYVVVNTRIMT
                     RMGKDVLPYLDTKGYVKCLHTIGAPLQPGQKDVTWPCNPDVKYISHFPEERSIVSYGS
                     GYGGNALMGKKCFALRIASVMGREEEWLADHMLIMGVESPEKEKTYVAAAFPSACGKT
                     NFAMLIPPKEFDGWKVTTVGDDIAWIHKGDDGNLYAINPEAGYFGVAPGTNYDTNPNA
                     MESIKGNTIFTNVAITADKDVWWEGLTKEVPDGLTDWHGKPYDKNSGKPAAHPNSRFT
                     APAYQNPAIDKDWDNPKGVPVAGFIFGGRRSTGVPLVYQSFNWNFGVYLATTMGSEMT
                     AAAFGEIGKVRRDPFAMLPFMGYHIGDYVNHWLQFGRSLPNPPRIFGVNWFRKDENGK
                     FLWPGFGQNIRVLKWIVERIKGKAPSVESPIGWMPRYEDIDWTGLKFSKEDFEKIMTV
                     DREPGKQELLSHEELFERMYDKLPKEIFFMRELLLSALWRSPEHWGLQPEKY"
     gene            3489..5159
                     /locus_tag="PK977_05600"
     CDS             3489..5159
                     /locus_tag="PK977_05600"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HRF17619.1"
                     /translation="MFHWLQQLFTEPSIAQSVIIYGLVIASGIWLGRIRIAGISLGIT
                     WVLFAGLIISYTGVEIEKSTEHFIKEFGLILFVYSIGLQVGPGFWASLKKNAMTNNLL
                     ALAVVATGVLITVILYYTSHNHISVMAGVMSGAVTNTPGLGAAQAAVSDLHITGTDKS
                     LITLAYAVTYPLGVFGIIGSLLLLKKIFRVNIAEEQELHRKLEVLRANRPVSIHLQLE
                     NKQLVGQPLRKIFELLKEPIVVSRMYHNGEIITPTPDIELAQNDVLLIVASKQEVEQL
                     KLLVGPVSNMNLKTAKESDLISRHIVVTRAAVTHKRLGDIPELQQHDFTLTRLSRAGI
                     EMVPHGDIFLQLGDTVKVVGTEEGVEIVADALGNSLKRLEAPDLAPIFMGIVMGVILG
                     SIPFHFPNMPVAVKIGMAGGPLIVALILSRFGNLFYLNNYTTNSANLMIRELGISLFL
                     ASVGLSSGKNLSHAFADGSGFGWIAMGIAITIIPLLLVGFIAHKFFRKTYFEVCGLLA
                     GASTDPPALAFATKLAGNDIPSVTYATVYPLTMILRIVAAQLLILLLA"
     gene            complement(5163..5516)
                     /locus_tag="PK977_05605"
     CDS             complement(5163..5516)
                     /locus_tag="PK977_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRF17620.1"
                     /translation="MKKIYHLGNCTTCQAIIKQTGIDKKSFDMQEIKTEKITASQLDE
                     MKKLAGSFEPLFSRRAIKYKEMGLKDKPLTETDYRKLILDEYTFLKRPVVIINNKIFI
                     GSEKKNVEALKQALK"
     gene            5598..6326
                     /gene="cmoA"
                     /locus_tag="PK977_05610"
     CDS             5598..6326
                     /gene="cmoA"
                     /locus_tag="PK977_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HRF17621.1"
                     /translation="MAKDEVFRDEIEKVSDFKFGANVAKVFDDMVNRSVPFYGEIQRM
                     MAELAADHAREETFVYDLGCSTGTTMIGMDTMVDQDIKFIGIDDSQEMLDKCKSKLME
                     IGFSRPYELRCADLGQGIKIENASVVVLCLTLQFVRPIYRERLLKDIYNGLNPGGVLI
                     LVEKILAEENNFNRDFINYYYNYKRRNNYSELEISQKREALENVLVPYKLSENITLLR
                     DQGFAHCEVFFKWYNFAGLVAVKK"
     gene            6332..7054
                     /locus_tag="PK977_05615"
     CDS             6332..7054
                     /locus_tag="PK977_05615"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRF17622.1"
                     /translation="MVSIGNFFFKHRNWIFILFYGALFIPSWPLFSSANFGNNYFIWP
                     IAIGLLVTILGQLIRGLTIGLAYIVRGGKEGKPYAEGLVTEGIFRHCRNPLYVGNILM
                     LLGVGILANSLVYVAIVIPVFLFIYQAIVLAEENFLRGKFGSGFDEYCKKVNRWFPNI
                     KGLGQTLNSMEFKWKRWILKEHTTQFIWLCGIVLILLLNYPQLSGYDKNHRNLLLGIF
                     LGVLVVVYAIVRYLKKSGKMKE"
     gene            7153..7749
                     /locus_tag="PK977_05620"
     CDS             7153..7749
                     /locus_tag="PK977_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRF17623.1"
                     /translation="MIKTGNPIISIYTEMTPNPETMKFVANKLLYPGKSIDFPDAESA
                     KPSPLATELFGFPFIKAVFIASNFVTLTKTADTDWQDVIPSVRQFLKDYLEEGKAVVN
                     EEELAAVKQESSNEVAADDDDVVKRIKELLDNYVRPAVEMDGGAIQFKSYNEGVVNLM
                     MQGSCSGCPSSMITLKAGIEGMMKRMIPEVKEVVAEAE"
     gene            complement(7818..8480)
                     /locus_tag="PK977_05625"
     CDS             complement(7818..8480)
                     /locus_tag="PK977_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRF17624.1"
                     /translation="MRKTFLTLASLAICLLSFAQDKAAKEKKKPTLSFTAGPSIPIGS
                     FGESDPLNVSSGMAKMGTSFSATYTHFIQKDFGIAVSFLVNWYETDTLLFSQGRASKP
                     GDNWQMVGFSVGPAFSRSFSKKLEGDFRLTGGFMNVNTPVLTYALRTIKKDTRFTPVL
                     QAEAGLNYSIGKKIYLLLNAAYMHMDPKFDAVLTINGNAADPYQQSISVINIRAGLGV
                     RL"
     gene            complement(8676..>10601)
                     /locus_tag="PK977_05630"
     CDS             complement(8676..>10601)
                     /locus_tag="PK977_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF17625.1"
                     /translation="NNQTWDNGVLFRLSHYYRVTGQYNEAVYYAEKHYQIAALKDQDD
                     KTLFAPRIILLFACLHAGQYSKAEEMIAAMNKSSLKYMLNNLDAFSDAEREKYLSKIN
                     RNLHLSNTMLLRNKNATPAFLKESFNELLLLKSLILAENRKLIESVIGGSDTTLQRLY
                     GQWLSTRNLLSREYAKPVKERRGDIIQLEEQAESLQKEMNQKSAFLGNRQRSKTISVS
                     DVQSALQEEEAAIEFVSFVVTNERNDSIMYAAHLLLKNNPVPVFIPLCTEKQLQKLFD
                     SAGSTATGMVNRIYRGIDLGTNNTAVSLGKELYNLIWAPLEPHLKGIKKISYTPSRKL
                     FNIAFQALPADSSLLLMDKYEFRQYFSTKQVVLRNEKVTTNTTGTLQPKNIFLFGDAS
                     FNVAGKTTSKQPMKNKGTSGFYLPASRGNQNSLWTDLPGTAEEVKKIKKQFELNKVPA
                     AVFTQTAASEENLKALSGNSPQILHIATHGFFLPEQEKKSDDIVTNNQNAYTLANDPL
                     LRSGLILSGGNHVWSGKTPIEGVEDGIATAYEISQLNLSNTELVVLSACETALGDVRG
                     SEGVFGLQRAFKMAGVKKMIVSLWQVPDKETAELMTSFYNYWLKGKTIEQAFAQAQSD
                     MRKKYSPYYWAAFVLVE"
     assembly_gap    10602..10611
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10612..12530)
                     /locus_tag="PK977_05635"
     CDS             complement(<10612..12530)
                     /locus_tag="PK977_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF17626.1"
                     /translation="MRTIRILSCLISFMLLGASRVKAQTWEQLNDSFHYYFEREDYKQ
                     ALPVAQKIVDVARNDFGEADQQYATALNNLAYTFYKTGNLAAAEDNFLECFTVKLRVY
                     EPEDERCLSTAQMLSDIYKEQQNYKEAEKLLTGYLQILSKKNKQQSKAIVYSLFMRGD
                     SYRVLQQNDRAITDFLGVLKNEKEFITDTSLLYGAYEALADLFGDVSDLQKGERIFLR
                     HTEFCKAVDGDQSEKYVLSLFRQAAFYGRAKQYLQSQRTLDKVVDWYSKNKGMESEEY
                     LSAYNNSMLLLCKLGNYAAADSMMDVQLDRLKKKLGESSPAFENAVHKAGMIFMQAEN
                     QSKAEEYFKKSIAISRRLNNPALLMSRLNVFDSLNVNSREERIFILEEMVALHEKHKL
                     LTDSAYATKLLFLGGSYERSGYSDKAVNLVPKMDNAAKKGYKERSNGFIDSRTIIANI
                     YVSVGMLDKADSYYQQSLKLAKEVWKDRKDRYAGLLKKVGQMYSYAGFDKKAEEILMM
                     ARQWMEESQSTDYSDWSSVLYELATVKSTLKKTTEAESAFRAAVNYAKKDTALFYEYL
                     QYAILFAEFFTDNKLFDKADSLYREITGVTNYPGKQSYNVFLSTLSAWGLCKYKSGDT
                     VNAARIIDSVVQLSHR"
     gene            complement(12535..13185)
                     /locus_tag="PK977_05640"
     CDS             complement(12535..13185)
                     /locus_tag="PK977_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17627.1"
                     /translation="MKKIGFFVLATTLLMTQLRAQYGSVSVFEKNAEQEVTSVRIFPV
                     KCTVTKMDDSAYLNLRKSINAFMHLDGSRVYTQFYIPFTGYDGAREFAIDEYIGKKAA
                     KKVKFIREFEEILLDNGGNVVSTQVKERPPGIMVIYYADFSKTADATVINSAYAYTVY
                     SKTPINAQEVAKTLAGRVVGSSKYTYEWLPGKTMEYAKNKIGPGITFRDNGEFRIN"
     gene            complement(13202..>13617)
                     /locus_tag="PK977_05645"
     CDS             complement(13202..>13617)
                     /locus_tag="PK977_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF17628.1"
                     /translation="NDPLLRSGLILSGGNYAWSGKAPIEGVEDGIATAYEISQLNLSN
                     TELVVLSACETALGDVKGSEGVFGLQRAFKMAGVKKMIVSLWQVPDKETAELMTSFYN
                     YWLKGKTIEQAFAQAQSDMRKKYSPYYWAAFVLVE"
BASE COUNT         3744 a         2890 c         2863 g         4064 t
ORIGIN      
        1 tcgcacaaga agatactaca cagcctcctg caactgacac tacagctgca cctgcagaag
       61 aacctccggc tgaggaagaa gagccttcac tcatttctcc ttctattgat tttaccagtg
      121 tgcaaaaagg tgataactcc attgacctga aggtgggtat gcgggccaag gtaaaaggcc
      181 agttcatcaa cctttataaa atgaaggttt cctttttcca ggtggtaaat gaagaagata
      241 taccattagg ttttaaaata accgatggga acggaaagga catttttnnn nnnnnnnnna
      301 gattcattaa aatcagatgc agaaggaaaa ttaaatttta aagtggtttt tgccggaaac
      361 aaagcgatgg aatctgccga agaatatgtt tcattccgca ggggcaggct ggagatcgtt
      421 ccgttaaaag aagattcatt gcttaccgta cagataaaat taattgatat cggaaccggg
      481 gaagaaacgc cggtaaaaga aacggctgtt ggcgtttttg tcaaaagaat gttccttcct
      541 atgaaagtag gggaggccac tactgatgaa aacggcgaag gctctgttga gttccctaac
      601 aacctgcccg gcgatgccaa aggtaacctg acgttgctgg cccgcctaga tgaaaacgaa
      661 acctatggca atatggcagc cgctgtagta cagccatggg gggttccggt ttcagacaag
      721 cccgaagctc agccccgatc attatggagc tctcacccgc cactgtggat gctgattacc
      781 ttcatcgtac tgatgctggc ggtatggggc cattatattg ttattgtgta tgaacttttc
      841 cggttaagaa aagaacagcc aaatgcacca actcatgcaa ctaattcgta acttaaacat
      901 tctttaatac gtaaaaaaca acaaacatga aaacaaaagc acctgttttg attgccacac
      961 tggtagcctt tgcattattt gctttcgctc ctaaatcnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn naaaacaaag taaatcctat taaaggcgat gcagcctctt tggaaaaggg
     1081 caaagctttg tggagccagc attgtaaatc ttgtcatggt tcaaaaggaa aaggagacgg
     1141 tcctaaagct tctcaattag acacagaacc cggtgatttt accaaagcga gtttccagtc
     1201 acaaactgac ggcgctttgt attataaaac aaatgccgga agagacgata tgccttctta
     1261 taaaacaaag atccctgatg ccaacgacaa ctgggccctt gttaactaca tgagaacatt
     1321 caaataatgg ttgataataa aatatataag acccggccga tggccgggtt ttttattgtc
     1381 catatgcatg ctatatgatt tacttcatac cgcttattga ccacgaacag tttaactctt
     1441 tgaaattgta ctcattttta cttcactgaa aaatcttcga cggtttcagt acgattgcct
     1501 gcctttttgt aaattccttt tttaaatact tatttatgtc aacacaaact aacttctttg
     1561 ccatggagac tgctccggca agggcagaac tcgaaacatg ggtaaaaaag gttgctgaat
     1621 tttgcgaagc agatgctgta cactggtgcg acggatcaaa agaggaatat gaccgcatgt
     1681 gccagctact ggtagataaa ggaacattta ttccgctaaa cccggaaaaa aggcccaata
     1741 gttttgcctg cttcagtgat ccaagtgatg tagcaagagt agaagacaaa acgtttatct
     1801 gcagtttgcg caaagaagat gcaggtccta ccaacaactg gatggatcct aacgaaatga
     1861 aaaaaatcct ggaaggcctg atgaagggaa gtatgaaggg ccgcacactg tatgttattc
     1921 ccttttgtat gggaccattg ggctctgctt attcaagata cggtgtacag attaccgact
     1981 cggaatatgt ggtagtgaat acccgcatta tgacccgcat gggaaaagat gtattgcctt
     2041 acctggatac aaaaggatat gtaaaatgcc tgcacactat cggcgcccca ctgcagccgg
     2101 ggcaaaaaga tgtaacatgg ccctgtaacc ctgatgttaa atatatttct cattttcccg
     2161 aagaaagatc aattgtctct tacggcagcg gctatggtgg taatgcgtta atgggtaaaa
     2221 aatgttttgc gctgcggatc gcttctgtta tgggaagaga agaagaatgg ctggcagacc
     2281 atatgctcat aatgggtgtt gaatctcctg aaaaagaaaa gacctatgta gcagctgcct
     2341 tccccagcgc ctgcggcaaa acaaactttg cgatgcttat tccgccaaaa gagtttgatg
     2401 gctggaaagt aactaccgtt ggtgatgata ttgcctggat ccataaagga gatgatggta
     2461 atctttatgc catcaaccct gaggcaggtt attttggtgt ggctcccggc accaactatg
     2521 ataccaaccc caatgccatg gagagcatca aaggcaacac catatttacc aatgtggcta
     2581 ttactgctga taaagatgtg tggtgggaag gcttgactaa agaagtgcct gatggtttga
     2641 ccgactggca tggcaagcct tatgataaaa acagtggcaa acctgctgca caccctaact
     2701 caagatttac cgcaccggcc tatcaaaacc ctgcgataga caaagactgg gataatccaa
     2761 aaggtgttcc ggttgccggt ttcatattcg gcggaagaag aagtaccgga gtacccctgg
     2821 tgtatcaatc attcaactgg aactttggtg tgtacctggc cactactatg ggcagcgaaa
     2881 tgacagctgc tgcttttgga gaaataggta aagttcgtcg tgaccccttt gccatgttgc
     2941 cattcatggg ttaccatatt ggtgattatg taaatcactg gttgcagttt ggccgcagcc
     3001 tgcccaatcc tccccgtatt ttcggtgtga actggttccg caaagatgaa aatggtaaat
     3061 tcctctggcc gggctttggt cagaatatcc gtgtgctgaa gtggatcgtg gagagaataa
     3121 aaggcaaggc tccatcagta gaaagcccta ttggctggat gccgagatat gaagatattg
     3181 actggacagg tttgaaattt tcaaaagaag attttgaaaa gatcatgacg gtggaccgtg
     3241 agccgggtaa acaggagttg ttatcccacg aagagttatt tgaaagaatg tatgataaac
     3301 tgcctaaaga gattttcttt atgcgggaac tcttgttgtc tgccctctgg cgttcaccgg
     3361 aacactgggg cctgcagcca gaaaaatact agcctctgat gattgcctgc ttatacaaac
     3421 aaatatactg aacgggtaac gatcggttgc ccgttcagtt gctgttaact tacttttgaa
     3481 tcctttatat gtttcactgg ctgcaacaac tctttacaga accctctatt gcacaatctg
     3541 tgatcatcta tggattggtg atagcctcag gcatctggct gggccgcata cgtattgcag
     3601 gcatttcatt gggtataaca tgggtattgt ttgcagggtt aattatttct tatacaggtg
     3661 tggaaataga aaaaagtact gagcacttta ttaaggagtt tggtcttatc ctttttgttt
     3721 actctattgg tctgcaggta ggtcccggtt tttgggcatc gctgaaaaaa aatgccatga
     3781 ccaataacct gcttgctttg gcggtagtag ccaccggtgt attgattacc gttattcttt
     3841 attataccag ccataaccat atttccgtaa tggccggcgt aatgagtggt gctgttacca
     3901 atactcccgg tttaggtgct gcacaggccg ctgtaagtga tttgcatatt accggtaccg
     3961 ataagtcgct catcacactg gcgtacgccg tcacttaccc gttgggagtt ttcggcatca
     4021 ttggttcatt attgctattg aagaagatat tccgggtgaa tattgcagaa gaacaggagc
     4081 tgcaccggaa gctagaagta ttgcgggcca acagacctgt atccattcat ttacaattgg
     4141 agaacaaaca actggtggga cagccgcttc gcaaaatatt tgaattgttg aaagagccga
     4201 ttgtcgtttc ccgtatgtac cacaacggtg aaatcataac tcctacacct gatattgaac
     4261 tggcacaaaa tgatgtgctg ctgattgtgg catcaaagca ggaagtggag caattgaaat
     4321 tactcgtagg cccagtcagc aatatgaatc taaaaacagc caaggaaagt gacctgatat
     4381 caagacatat tgttgtaaca agagcagccg ttacccacaa acgcctgggc gatattcccg
     4441 aactgcagca acatgatttc acattgaccc gcctcagcag ggctggtata gaaatggtcc
     4501 cccatggcga tattttttta caactgggcg ataccgtaaa agtagtgggc acggaagaag
     4561 gggttgaaat agttgccgat gcgttgggca attcattgaa acgtcttgaa gcccctgacc
     4621 tggccccaat atttatgggc attgtaatgg gtgtgatact tggcagtatt ccgtttcatt
     4681 ttcccaatat gccggttgcc gtaaagatcg gcatggctgg cggcccgctg atcgtagcat
     4741 tgatattgag ccgctttggt aacctgttct acttaaacaa ttacactacc aacagtgcca
     4801 acctgatgat cagggagctg ggcatcagcc tttttctcgc cagtgtgggg cttagcagcg
     4861 ggaagaacct gtctcatgca tttgcggatg gcagcgggtt tggctggatt gctatgggca
     4921 ttgccattac catcattccg ttgctgttgg ttggctttat cgcccataaa tttttccgca
     4981 aaacttattt tgaagtctgt ggcctgctgg ccggcgcctc taccgatcct ccggcactgg
     5041 cctttgccac caaactggca ggtaatgata ttccatcagt cacctatgcc actgtatacc
     5101 cgctgacaat gatcctgcgg atagtagccg cccaattatt gattttgcta ttggcctgat
     5161 aactatttta atgcctgctt aagtgcctct acatttttct tttcactgcc aataaatatt
     5221 ttgttgttga tgatgacaac aggcctcttc agaaaagtgt attcgtccag tatcagctta
     5281 cggtaatctg tttcggtgag gggtttgtcc ttcaaaccca tttccttgta cttgatcgcc
     5341 ctgcggctga acaagggttc aaaactgcca gccagctttt tcatttcatc taactgggaa
     5401 gccgtaattt tttcagtctt tatttcctgc atatcaaagc tctttttatc aatgcctgtt
     5461 tgtttgatga tggcctggca ggtagtgcaa tttccgagat gatatatttt tttcatataa
     5521 atattaatac aacgcaagat aacctccggt tcactaccgt ataaattgct ttactttgta
     5581 tcccaatttt tttgattatg gcaaaagacg aagtgtttcg ggacgaaata gaaaaggtca
     5641 gtgactttaa attcggcgcc aatgtggcca aagtgtttga tgacatggta aaccgctccg
     5701 ttccgttcta tggagaaatt caacgaatga tggctgagct agccgccgat catgcaaggg
     5761 aagaaacctt tgtatatgat ttgggttgct ctaccggtac tacgatgatc ggtatggata
     5821 cgatggtgga ccaggacata aaattcatcg gcattgatga ttcacaggaa atgctggata
     5881 agtgcaagtc taaattgatg gagataggat tttcccgtcc ttatgaatta cgatgtgctg
     5941 atcttggaca aggtattaaa atagaaaatg catctgtagt agtgctttgt cttaccctgc
     6001 aatttgtaag acccatttac agggagcggc tcttaaaaga tatttacaat gggcttaatc
     6061 ccggcggtgt gctgatactg gtagagaaaa tattagccga agagaataat ttcaaccggg
     6121 actttatcaa ttactattac aattacaaac gcagaaacaa ttacagcgaa ctggaaatct
     6181 cccaaaaaag agaagccctg gaaaatgtac ttgttccgta taaactatca gaaaatatta
     6241 ctttgttgcg tgaccaggga tttgctcatt gcgaggtctt ttttaaatgg tacaattttg
     6301 cagggttggt tgcagtcaaa aaataaaagg catggttagt atcgggaatt tctttttcaa
     6361 acaccggaac tggattttta ttttatttta tggggccttg ttcattcctt cctggccttt
     6421 attttcttcc gcaaactttg gaaataatta ttttatctgg cccattgcaa tcggtttatt
     6481 agtaaccatt ttaggtcagc tgatccgtgg cctcaccata ggactggcct atattgttag
     6541 gggcggaaag gaaggcaagc cttacgctga aggattggta acggaaggga tctttcgtca
     6601 ttgccgcaat ccactatatg tcggcaatat cctgatgctg cttggtgtgg gtattttggc
     6661 caattcgctg gtgtatgttg ccattgtaat tccggttttt ttattcattt accaggccat
     6721 tgttttggcc gaagagaatt ttctccgggg taaattcggc agtggctttg atgaatattg
     6781 taaaaaggta aatcgttggt ttcccaatat aaaagggctt ggacaaacgc tgaatagcat
     6841 ggaattcaaa tggaagcgat ggattttgaa agaacatact acccagttca tctggctttg
     6901 tggtattgta ctaatacttt tactcaacta tccccagctc agcgggtatg acaaaaatca
     6961 ccgcaacttg ttattaggta tttttctcgg cgtcctggtg gttgtttacg ctattgtccg
     7021 ctacttgaaa aaaagcggta aaatgaaaga ataagcagtt cgttttcagt acctccccct
     7081 aatcgtttcc tttcccgcct tcgtaaacat tggccccgca gaattgttat ttttgcatct
     7141 taaagtgagt ttatgattaa aacaggtaat cctatcatca gcatttatac cgaaatgacg
     7201 cctaacccgg aaaccatgaa gtttgtagcc aacaaacttc tgtatcccgg taaaagcatt
     7261 gatttcccgg atgcggaaag tgctaaacct tcgccactag cgaccgagtt atttggtttt
     7321 ccatttataa aagccgtttt cattgccagc aattttgtta cactgaccaa aacagcagat
     7381 acagactggc aggatgtaat accctctgtt cgccagtttt tgaaagatta cctggaagaa
     7441 ggcaaagccg tggtaaatga ggaagaactc gctgctgtaa aacaggaaag cagtaatgaa
     7501 gtggcggcag atgatgatga tgtggtaaaa cgtattaaag aactgcttga caattatgtt
     7561 cgccctgccg tagaaatgga cggtggtgcc attcagttca aaagttataa tgaaggagtg
     7621 gtgaacctga tgatgcaggg aagctgcagt ggctgtcctt catccatgat cactttaaaa
     7681 gccggcattg aaggcatgat gaagcggatg ataccggaag taaaagaagt agtggcggaa
     7741 gcagaataaa aaacttacaa aaaataaaaa aggctgtgtc gtttgataca gccttttttt
     7801 gtgctaaacc cttcacttta caatcttaca cctagccccg ccctaatatt gataacagaa
     7861 atgctttgct ggtaaggatc agccgcattg ccattgatag taagtactgc gtcaaacttt
     7921 gggtccatat gcatatacgc tgcattcaaa agaagataga tctttttacc aatggaataa
     7981 ttcaaccctg cttcagcctg cagaaccggc gtaaacctgg tatccttttt aattgttctc
     8041 agtgcataag taagtaccgg cgtatttaca ttcataaaac cgccggtgag gcgaaaatcc
     8101 ccttccagct tttttgaaaa actccgggaa aaagcaggcc cgacagaaaa gcccaccatc
     8161 tgccagttat cgccgggttt tgaagccctg ccctgggaaa aaagcagtgt atctgtttca
     8221 taccagttca ccaggaaaga aacggcgata ccaaaatcct tttgaataaa atgagtgtat
     8281 gtagctgaga aactggttcc cattttagcc ataccactgc tgacattcag ggggtcgctt
     8341 tctccaaaag aaccaatcgg gattgaaggt ccagctgtga aagataatgt tggtttcttt
     8401 ttttctttgg ctgctttatc ctgtgcaaag gacaacaggc aaatagctag tgaggctagt
     8461 gttaaaaatg tttttctcat gatggttatt ttggttagta aacaatctgt tcaggcacag
     8521 atactacaat gtaattatgt aacaaagtaa acattaattt gatacaatgc caatgactat
     8581 tccggcaagc tttgcggtat tgtgctggcg gttagactat aatatcagca agtgttaata
     8641 aacaaagcaa aaacaggaaa tccggatatg ctgatctact ccactaatac aaacgctgcc
     8701 caataatacg gtgaatattt ttttcgcata tccgattgcg cctgtgcaaa agcctgttca
     8761 atcgttttgc cttttaacca gtagttgtaa aagctggtca tcaactctgc cgtctcttta
     8821 tccggtacct gccacaggct gacgatcatc tttttaaccc cggccatttt aaatgctctt
     8881 tgtaaaccaa acacaccttc acttcctctt acatctccca gtgctgtttc gcaggcgctg
     8941 agtacgacca gttctgtgtt gcttaggttg agttgtgaaa tttcataagc ggtagcaatg
     9001 ccatcttcta ctccttcaat gggtgttttg ccactccaaa catgatttcc gccggataaa
     9061 atcagaccgc tgcgcagcaa ggggtcattg gccagtgtgt aagcattttg attattggtg
     9121 acaatatcat ccgatttttt ttcctgttcg ggtaaaaaga agccgtgtgt ggcaatatgc
     9181 agtatttgcg gtgaattacc gcttaatgct ttcaggtttt cctcactggc tgcagtctgt
     9241 gtgaaaacag cagcaggtac cttatttaac tcaaattgtt ttttgatctt ttttacctct
     9301 tctgcagtac cgggcaggtc agtccataaa ctattctgat tacccctgct tgcaggcagg
     9361 taaaagcctg atgtgccttt attcttcatc ggctgcttgc ttgtagtttt accggcaaca
     9421 ttgaaactgg catcgccaaa caaaaaaatg tttttgggct gaagggtacc tgttgtgtta
     9481 gttgttacct tttcatttcg caataccacc tgtttggtac tgaaatactg gcgaaactca
     9541 tatttatcca tcagcaacag gctgctgtct gcaggtaatg cttgaaatgc aatattgaaa
     9601 agtttgcggg aaggggtgta actaattttt ttaattcctt tcaggtgtgg ctccagcggc
     9661 gcccatatta aattgtatag ctccttgccc aaagaaacag cagtattgtt agtgcctaaa
     9721 tcaatacccc gataaattcg attgaccata ccggtagcag tagacccggc actgtcaaaa
     9781 agtttttgca gttgcttttc cgtacaaagt ggtataaata caggaacagg attatttttt
     9841 aacaatagat gagccgcata cattatactg tcattccttt cattggttac aacaaaagaa
     9901 acaaactcga tagctgcttc ttcttcctgc aaagcagatt gtacatcact taccgaaatt
     9961 gttttactcc gttgcctgtt tcctaaaaat gctgattttt gattcatttc tttctgcagg
    10021 ctttctgcct gttcttccag ctgtataata tctcccctcc tttctttcac aggttttgca
    10081 tattcacggg acaataaatt acgggttgac aaccattgac cataaagcct ttgcaaagta
    10141 gtatcactgc ccccaataac tgattcaatc agtttacggt tttctgccag tatcagggat
    10201 tttagtaata ataattcatt gaaactttct ttcagaaatg ccggtgtggc gtttttgttc
    10261 cgcagcaaca tggtatttga taaatgtaaa ttcctgttta ttttggacaa atacttttcc
    10321 ctttcggcat ccgaaaaagc atccaggtta ttgagcatat acttcaacga tgatttgttc
    10381 atcgctgcaa tcatctcttc tgccttgcta tactggccgg catgaaggca ggcaaacagc
    10441 aggataattc ttggtgcaaa caatgtttta tcatcctggt ccttaagagc ggcaatttga
    10501 taatgctttt cagcataata aacagcttca ttatattgtc ctgtaaccct gtaataatga
    10561 gataaccgaa agagaacccc attgtcccag gtttgattgt tnnnnnnnnn ncgatgagac
    10621 aactgaacta ccgagtcaat aattctggca gcgttgaccg tatccccgga tttatatttg
    10681 catagacccc aggccgataa agtggaaaga aatacattgt aagattgttt tcccgggtag
    10741 tttgtaacac cggtaatttc cctgtataag ctatcggctt tatcaaatag tttattgtcg
    10801 gtaaaaaatt cggcgaaaag tattgcgtac tgcagatatt cataaaaaag ggccgtatct
    10861 ttttttgcat agttcactgc agccctgaag gccgactcag cttctgttgt ttttttcagc
    10921 gtacttttta cagttgcgag ttcataaaga acagatgacc agtcgctata atcggtactt
    10981 tggctttctt ccatccattg acgagccatc atcagtattt cttcggcttt tttatcaaaa
    11041 ccggcgtaac tatacatttg gcctactttt tttagtaacc cggcatacct gtcctttctg
    11101 tctttccata cttcttttgc cagtttgagt gactgttgat aataactgtc cgctttatcc
    11161 agcatcccga cgcttacata aatattggca atgatggtgc gggaatcaat aaagccattt
    11221 gaacgttcct tgtacccctt ctttgctgcg ttgtccattt ttggcaccag gtttacagct
    11281 ttatcactgt atccgcttct ctcgtaagaa ccgccaagga aaagcaattt ggttgcataa
    11341 gctgaatcgg taagtagttt atgcttttcg tgcagcgcaa ccatttcttc taaaataaag
    11401 atcctttctt ctcggctgtt aacattcagg gaatcgaaca catttagccg gctcatcaac
    11461 aatgcgggat tatttaaccg gcggcttatg gcaatacttt tcttaaaata ttcttctgcc
    11521 ttggattgat tttcagcctg cataaaaatc atgcctgctt tgtgaacggc attttcaaag
    11581 gccggtgagc tttcacccaa cttttttttc aatcgatcca attgcacatc catcatactg
    11641 tcggcagcag catagtttcc cagtttacac agcaataaca tactgttgtt gtaagcagaa
    11701 agatattctt cactttccat gcctttgttc ttactgtacc aatcaactac tttgtcgagg
    11761 gttcgctgtg attgtaaata ttgcttagcc ctcccataaa atgccgcctg gcgaaacagg
    11821 gaaagcacat atttttcact ttgatctcca tctaccgctt tacaaaattc tgtatgtctc
    11881 aagaagatcc tttctccttt ctgcaggtct gatacatccc caaacaaatc agccagcgct
    11941 tcgtaggcac catacaaaag ggaagtatct gtaataaact ctttttcatt tttcaaaacc
    12001 ccaagaaaat ctgttatagc ccggtcattc tgttgtaata cccggtagct gtcgccccgc
    12061 ataaaaagag aatagacaat tgcttttgac tgctgcttat ttttctttga aagaatttgc
    12121 aaataaccgg ttaatagttt ttcggcctcc ttatagtttt gttgctcctt ataaatatca
    12181 ctcagcattt gtgctgtact aagacagcgt tcatcttcag gttcatatac tctcaatttt
    12241 acagtaaaac attccaggaa attatcttct gctgctgcaa ggttgccggt tttataaaaa
    12301 gtgtaagcaa gattattaag cgcagtggca tattgctgat cggcttcacc aaaatcattt
    12361 cgggcaacgt ccacaatttt ttgtgctaca ggcagggcct gtttataatc ttctctttcg
    12421 aagtaataat gaaaactgtc gttcagttgc tcccaggttt gtgcttttac acggctggca
    12481 cctaatagca tgaaagaaat caggcaagat aaaatacgga tagttctcac ttgttcagtt
    12541 tattctgaat tccccgttat cccgaaatgt tattccgggc ccaattttat ttttcgcata
    12601 ctccattgtt tttcccggca gccactcata agtatatttt gagctgccaa ctacccggcc
    12661 cgccaatgtt ttggccactt cctgtgcatt aatcggggtt ttgctataaa cagtataggc
    12721 atatgcagaa tttataacag tggcatcggc agttttacta aaatcagcat aataaataac
    12781 cataatgccg ggcggtcttt cttttacctg tgtgctaacc acatttcctc cattgtcaag
    12841 caaaatttct tcaaactccc ggatgaactt tacttttttt gctgcttttt tgccgatata
    12901 ctcatctatt gcaaattctc tggccccgtc atatcctgta aacgggatat agaattgagt
    12961 atataccctt gaaccatcga ggtgcataaa agcattgata cttttccgga ggttcaggta
    13021 agcgctgtca tccatcttgg tgacagtaca ttttacagga aatatccgga cgctggttac
    13081 ctcttgctca gcattttttt caaaaacgct cacactacca tactgtgccc gcagttgtgt
    13141 catcaacaat gttgttgcta aaacaaaaaa tccgattttt ttcattctca tttttatttt
    13201 tttattccac caatacaaac gctgcccaat aatacggtga atattttttt cgcatatccg
    13261 attgcgcctg tgcaaaagcc tgttcaatcg ttttgccttt taaccaatag ttgtaaaagc
    13321 ttgtcatcag ttcggccgtt tctttatccg gcacctgcca caggctgacg atcattttct
    13381 ttactcccgc cattttaaag gctctttgta aaccaaatac cccttcgctg ccctttacat
    13441 cacccagtgc tgtttcgcag gcactaagta cgacgagctc agtattgcta aggttgagtt
    13501 gagatatttc gtacgccgtt gcaatgccgt cttctacccc ttcaatgggt gccttgccgc
    13561 tccatgcata gttgcctccg gataaaatta agccgcttct aagcaggggg tcattag
//
LOCUS       DAORLS010000049        13502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2439_length_13502_cov_1.798468, whole genome shotgun sequence.
ACCESSION   DAORLS010000049 DAORLS010000000
VERSION     DAORLS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_13502_cov_1.798468"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PK977_05650"
     CDS             <1..1093
                     /locus_tag="PK977_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRF17629.1"
                     /translation="TAMGKRPVKSIIRQSSTGHATNVIGGLAVGMESTFLPILVLAAG
                     IWGSYECAGLYGVAIAAAGMMATTAMQLAIDAFGPIADNAGGIAEMSELPKEVREKTD
                     VLDAVGNTTAATGKGFAIASAALTALALFAAFVGVAGIDGIDIYKAKVLASLFVGGMI
                     PLIFSSLAIRAVGEAAMSMVTEVRRQFHSIPGIMEGKGKPEYDKCVAISTNASIKKMM
                     LPGAITIVSPLIVGFALGPEALGGFLAGATVSGVLMGMFQNNAGGAWDNAKKSFEKGV
                     EINGQMYYKKSDPHKASVTGDTVGDPFKDTSGPSMNILIKLMSIVSLVIAPTLAQMYS
                     EKKTEEEVKKVEIKSEPVTVVPKAHTVQL"
     assembly_gap    1134..1312
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1313..>2507
                     /locus_tag="PK977_05655"
     CDS             <1313..>2507
                     /locus_tag="PK977_05655"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRF17630.1"
                     /translation="QPETEDVDKPIDEKEKKKKVTDRDFSVTKENAYNDIFLDSMAME
                     NYISRRQLTDKKISRRIRSFYNARNYQYAWFTSTGLTEQTRFFWNQYDYAVTHLKDSS
                     LVNSDFYKNAEKYLNQEKVAVSTKDSALLYAEFGFTEHFIRFINSTYEKGYVKRKEQE
                     KFVPIKKADPVYLADSLLNKKHSDDKYYEQVNDMYAALKNHLQLYLDIEKSGGFPQVP
                     VVKGSLKKGAEDPAIKVIKKRLQLSKDMPGTDTSSLFNDTLELAVKRFEKRHGYKDDG
                     VITGEIVKEMNVSAEKRLMQILLNMDRMRWMPQKPEGNLIIVNIPEFMLHVYDGKKRL
                     FDMVVVVGKVGNNTMMFNGDLSYIVFSPYWNVPPSIIKSEILPAMQRDPNYLARKNME
                     KVGNGI"
     assembly_gap    2508..2543
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2544..2946
                     /locus_tag="PK977_05660"
     CDS             <2544..2946
                     /locus_tag="PK977_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRF17631.1"
                     /translation="GKVKFIFPNSFNMYFHDTPSKSLFGQDKRAFSHGCIRLSEPQKM
                     AEWLLRNDPEWNTEKIVAAMNQTSEKMVKLKSLLPVFIIYYTAWVDDDGLLNFRDDVY
                     KHDSELISKMFTKKEAPKLIKVDNLQVSGSN"
     gene            3063..3371
                     /locus_tag="PK977_05665"
     CDS             3063..3371
                     /locus_tag="PK977_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17632.1"
                     /translation="MVRYIMSFKVPENSQQTVQQVVNQYFNELQRSGPGGMRSQCYAS
                     HDDQCNFVHIKSFKKESIANHHFRSAIFRQYLEKLAVIAGEPLSFSRLHQQKTFESIY
                     "
     gene            3481..4266
                     /locus_tag="PK977_05670"
     CDS             3481..4266
                     /locus_tag="PK977_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF17633.1"
                     /translation="MHRVIVPVDFSETSLNAARYTAQMLAGKDNALAVLYHNYESHDD
                     HDVSLNYQESLKKEFLKAGVKAVQTESEMGGDLIENISRLAHTIRASLIVMGITGKSA
                     IRAAMFGSNTLKLIDRNLYPVMIIPPDAKYKGIKNVAFASDFKNVEESTPAVLLDSVL
                     DLFKPQLHIFHVNKDISVSESAEVVGQKALFEQMFKKYDTRFHFLIKNDFYSAVDSFI
                     KDYEIDVMITIPKHQSNSMSLFKTTHTKRLAYHSHIPILAAHE"
     gene            4341..5126
                     /locus_tag="PK977_05675"
     CDS             4341..5126
                     /locus_tag="PK977_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF17634.1"
                     /translation="MKTVIVPVDFSETSLNAARYATKLLTGHYGVTMVLFNFCDNEKQ
                     IPEAIQSLENLRALLRESGIVKTEILAVTGPGFISELDRMSREVEADLVIMGITGRSP
                     IGQSLIGSNTLKMVEKRTCPVLIVPSSATYKDAKEVMLTSDFDNVEFTIPAQQIKNVL
                     NRLRPNLHIMNTDEEHYISITEEYKAEREKLASLFADFHPEFHFLTLNDVQDAIYQFA
                     QDKQIDLIIIVHREQTLFSKLFVKSNTKRLAYYSTIPVLAVHE"
     gene            5352..6299
                     /locus_tag="PK977_05680"
     CDS             5352..6299
                     /locus_tag="PK977_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRF17635.1"
                     /translation="MKNIKLIEVPSEIGAGTRGASLGIEAIKIAALDFMSNFFIHFPS
                     EKIPTENKLLYEPIESPYAKRIKGVAAIYDRVSKAVCDTLKNNFFPVILSGDHSTAGA
                     TIAGIKMAKPKSKLGVIWIDAHSDLHTPYTTPSGNVHGMPMAVSINKNNEECAVHDVD
                     DETAKYWNYLKNIGKIAPKVLPEDIVFISLRDYEKEERHLIEKYDMKVITTKEVRSKG
                     AENVVRSVHRYLADCTDIYISFDVDSLDSSISKGTGTPVSNGLKEREAEDLISKFMQN
                     RKVCCFEIAEVNPTLDKENLMAEIAFNILQRSVNVLMMN"
     gene            6309..6902
                     /locus_tag="PK977_05685"
     CDS             6309..6902
                     /locus_tag="PK977_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRF17636.1"
                     /translation="MQRNFLVAILLISAFPSLSQLGSYVDSINNYRSKYVKEHEVVKG
                     SDKKNMSFFPADETYRVITRVELIEDAPWFSMETSSDKKKPYRVYAILHFKLNNEAFK
                     LHVYQSKDLLKIAEYADHLFIPFIDETCGEDSYENGRYIDLTITDISQGTYFLDFNKA
                     YNPYCAYISNKYSCPVPPNENHLAVAIKAGEKKFGDH"
     gene            complement(6911..7801)
                     /locus_tag="PK977_05690"
     CDS             complement(6911..7801)
                     /locus_tag="PK977_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF17637.1"
                     /translation="MKVLITGANGFLGHYLTGQLLTKGIAVIATGKGECRLPYSDNRL
                     FQFQSLDFTDPEAVQSIIGNSRPDVIVHSGALSKPDECEQHKDYADKVNIDGTRHLLE
                     AAAAVQSFFIFISTDFIFDGKEGMYTEEDKGNPVNYYGLTKLRAEALVQQYVFNWSIV
                     RTVLVYGKPLTGRGNILTVVREKLEKGEEYSVFDDQVRTPTFVEDLAAGIVAIIQRRA
                     TGIFHLSGKDILTPYQMACETADYLGLNKLLIKKVTAAIFSQPAMRPAKTGFNIEKAR
                     RELGYDPVSFDEGIRKTFDT"
     gene            complement(7876..8562)
                     /locus_tag="PK977_05695"
     CDS             complement(7876..8562)
                     /locus_tag="PK977_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17638.1"
                     /translation="MSKGLLFGFALMAVSSLFGQASPETEATIDDLKVSPMRIPALQE
                     VSGSPFMTTEYKNATIHLKDNKYVADIPVKFNIYNNFMMVKMDDGQELKLDNFKSVTW
                     DEKADDGTLKHYEFAMGYPEIDKHSDRAVYQVLSAGPNAHLLKYISQKVEDANTLGDY
                     SRRELVKSEQLYLYIPGKDIKRIKASKKDIAEALPALASKIEEIAASNNLKLKSESEI
                     VTLVEALNKP"
     gene            complement(8689..>9543)
                     /locus_tag="PK977_05700"
     CDS             complement(8689..>9543)
                     /locus_tag="PK977_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF17639.1"
                     /translation="VKLKTDDFVTEQVFFTSKDGTKVPMFLTYKKGLVKNGQNPVLLY
                     GYGGFNIPVTPGFSTSNAFFIEQGGIYVSVTLRGGSEYGEKWHKAGMLENKQNVFDDF
                     IGAAEHLIKEKYTNSQKIAIRGGSNGGLLVGAVMTQRPDLFKVALPAVGVLDMLRYHK
                     FTVGWGWAVEYGNADSAKQFPYLYKYSPYHNLKQGVSYPATLVTTADHDDRVVPAHSF
                     KFAARLQEYHSGANPVLIRVDVNAGHGAGKPTSKQIDEATDIWSFVMYNLGMPFREPS
                     APVQKKGF"
     assembly_gap    9544..9612
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9613..>10005)
                     /locus_tag="PK977_05705"
     CDS             complement(<9613..>10005)
                     /locus_tag="PK977_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF17640.1"
                     /translation="PTQDKFNLLIKGFTTEPDVIDNYSNRLLVRTNDGAPNYKVVLID
                     PKNPGKENWKTIIPEKEEALQGVGTAGGYMFASYLKDASTKVYQYTYNGDLVREIKLP
                     GIGSAGGFGGEREDKEFFYTFSSFSTPP"
     assembly_gap    10006..10015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10016..>10305)
                     /locus_tag="PK977_05710"
     CDS             complement(<10016..>10305)
                     /locus_tag="PK977_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF17641.1"
                     /translation="DKIRWIKFSGFSWKGDDGFYYSGYDQPDEKTKLSKQNQNHKVFY
                     HKLGTDQSQDELIYQDKEHPLRYHSAGLTEDKRFLILQVSEGTSGAEIWYWD"
     assembly_gap    10306..10397
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10398..10852)
                     /locus_tag="PK977_05715"
     CDS             complement(<10398..10852)
                     /locus_tag="PK977_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF17642.1"
                     /translation="MKKHFAFLMFIAPLLAAGQLQYPTTKKGETVEDYHGTKVADPYR
                     WLEDDNSEETKAWVQAQNKVTFDYLATLPQRDKIKKRLEELWNYPRYSSPSKKNEYYY
                     FYKNDGLQNQAVLYRQKGLDGKPEEFLNPNTLNKEGIAALGSTSFSKSG"
     gene            complement(10965..11357)
                     /locus_tag="PK977_05720"
     CDS             complement(10965..11357)
                     /locus_tag="PK977_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17643.1"
                     /translation="MEIEIFTLADFAQDNNSKLTIVGTFDSINSKQFPAQHPACSLAC
                     RLRFAAKEAGPHDFKLRLINTDGKEIIQPIEGNINIGNPPNGQFASVNVVINFNQLQF
                     EKAGRYSFELYVDGEWKSGLPLLLNQVS"
     assembly_gap    11618..11729
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11820..>12037
                     /locus_tag="PK977_05725"
     CDS             11820..>12037
                     /locus_tag="PK977_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17644.1"
                     /translation="MGIKPRVRAAYRHDYVVAEFNNDSIRIIYPGSVRAYFVRWDETK
                     PEQVSWYVSDFIDVAYAMLIKPEKTKSS"
     assembly_gap    12038..12075
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12076..12303
                     /locus_tag="PK977_05730"
     CDS             <12076..12303
                     /locus_tag="PK977_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17645.1"
                     /translation="HLWYFEQPDPGSKNDQFFILEESNTGKRKYFTSTRWLKKQLSDW
                     PGINTKNTAYNNWFTGKQVIVKDYNRYKAGQ"
     gene            complement(12356..13207)
                     /locus_tag="PK977_05735"
     CDS             complement(12356..13207)
                     /locus_tag="PK977_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17646.1"
                     /translation="MKNLFSSNKPLLTESYTKAGINPVKKLSVQQPLTAYYNRVKTAG
                     KLILDRDLVREALATAGLVVTKIEKVPPSFFALWVFNNYPNREMPRPVNYSHESATSK
                     ISFTSRRCDGAEDCYQGVFAIGYPNNITVPKNPEIIKAKIVLEYSFMYTGWDTYGANT
                     AMELMLRTGNRFVSTQVNDLPNSSLIKPDFVYPSGTLITTPTDGWKTAGIMLPPVSVT
                     TDVQEIGARKNGTISFEGFVTPGSNFDVHLGIGYPPGTNKGLHGCYHYCEFLLKKMTV
                     TFLKSSR"
     gene            complement(13314..>13502)
                     /locus_tag="PK977_05740"
     CDS             complement(13314..>13502)
                     /locus_tag="PK977_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17647.1"
                     /translation="KFSMVGQVQPVQNIDNQYFSFHFIGVRSIFIKSKSHVADYIFSF
                     YFWSVTIHFSTGQLEKGI"
BASE COUNT         3851 a         2671 c         2678 g         3766 t
ORIGIN      
        1 tacagccatg ggtaaacgtc cggtgaagtc aattatccgt cagtcttcaa caggtcatgc
       61 taccaacgta atcggaggac tggccgtagg tatggaatca acctttttac ctatcctggt
      121 attggctgcg ggtatttggg gctcttatga gtgtgcaggg ctatatggtg tggcaattgc
      181 cgcagcaggt atgatggcga caacagctat gcaattagct attgatgcat ttggccccat
      241 cgctgataat gccggtggta tagctgagat gagtgagcta cccaaagaag tgagagaaaa
      301 aacagacgta ctggatgcag ttggtaatac cactgcagct acaggaaaag gctttgctat
      361 tgcatctgcg gcattgacag cccttgcatt gtttgcagcc ttcgtaggtg tagccggaat
      421 tgatggcatc gatatttata aagctaaagt attagcctcc ttgtttgtag gtggtatgat
      481 accgctgatc ttctcctcac tggctatccg tgctgtgggt gaagcagcta tgagcatggt
      541 tacagaagtt cgtcgccagt ttcattctat acccggcatt atggaaggaa aaggaaaacc
      601 tgagtatgat aaatgtgtgg caatttctac caatgcttct atcaaaaaaa tgatgttgcc
      661 gggagcaatc acaatcgttt caccattaat tgtaggcttt gcattaggtc ccgaagcatt
      721 gggtggtttt cttgccggcg ctacagtaag tggtgttttg atgggaatgt tccagaacaa
      781 tgccggtggt gcatgggata acgccaaaaa atcgtttgaa aaaggcgttg agatcaacgg
      841 acagatgtat tataaaaaat ctgatccgca taaagcttct gttacaggtg ataccgttgg
      901 agatcctttt aaagatactt ctggcccctc tatgaatatt ttgatcaaat tgatgtcaat
      961 tgtatctttg gtcatcgccc ctactcttgc acagatgtac agtgaaaaga agaccgagga
     1021 agaagttaag aaagttgaaa taaaatctga acccgttaca gttgttccca aagctcatac
     1081 agttcagtta taaataaaag cttaatcaga accattacta aagtcctgct gttnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccagcctg
     1321 agacagaaga tgttgacaag cccatcgatg aaaaagagaa aaagaaaaaa gttaccgaca
     1381 gggattttag cgttacgaag gaaaatgcct ataatgatat tttcctcgac agtatggcta
     1441 tggaaaacta tatcagccgc cgccagctga ccgataagaa gatatccagg agaatacgca
     1501 gtttctataa tgcccgcaat taccagtatg catggttcac ttcaaccggc ttaacggaac
     1561 aaacaagatt tttctggaac cagtacgatt atgcagtgac acatctgaaa gacagctcac
     1621 tggtgaacag tgatttttat aagaatgcag agaaatacct gaaccaggaa aaagtagcag
     1681 tcagtaccaa agactctgcc ctgttgtatg ctgaatttgg gtttactgaa catttcatac
     1741 gctttatcaa tagtacttac gaaaaaggct atgtaaaacg taaggagcag gaaaagtttg
     1801 ttcccatcaa gaaagctgat ccggtttacc tggccgactc attattaaat aagaaacata
     1861 gtgatgacaa gtattacgaa caggtgaatg atatgtatgc cgctttaaaa aaccacctgc
     1921 aactttattt ggatatcgaa aagtcaggcg gctttcctca ggttcctgtt gttaagggaa
     1981 gtttgaaaaa aggagctgaa gatccggcta taaaggtcat taagaaaaga ttgcaactat
     2041 ctaaagacat gccaggcaca gatacaagtt cattatttaa tgatacactg gaactggcag
     2101 taaaaaggtt tgaaaaaagg catggatata aagatgacgg ggttattacc ggggagattg
     2161 tgaaagaaat gaatgtaagt gcggagaaaa gattgatgca gatactgctg aatatggaca
     2221 ggatgcgttg gatgccacaa aaacctgagg gcaatcttat cattgtaaac atacccgaat
     2281 tcatgctgca tgtctatgat ggtaaaaagc gattatttga catggtagta gtggtgggaa
     2341 aagttggaaa caataccatg atgtttaatg gcgacctcag ttacattgtt ttcagcccat
     2401 attggaatgt gccgcccagc attataaaaa gtgagatatt acccgctatg caaagagatc
     2461 caaattatct tgcccgaaag aatatggaga aagtgggtaa tggtattnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnngggaaaa gtgaagttta tttttcccaa tagttttaat
     2581 atgtatttcc atgatactcc ttctaagagc ctttttggac aggataaaag ggcattcagt
     2641 cacggatgta tccgccttag cgaaccgcag aaaatggcgg aatggctgtt gaggaatgac
     2701 ccggaatgga atacagaaaa aatagtggct gctatgaacc agacatcaga gaaaatggta
     2761 aagctaaaat ccctgttacc tgtctttatc atttattaca ccgcttgggt ggatgatgac
     2821 ggcctgctca atttcaggga tgatgtatac aaacatgata gtgagctgat cagtaaaatg
     2881 ttcaccaaaa aagaagcccc gaaactaatt aaagtagata acctgcaggt ttcaggttct
     2941 aactgatact acgttaatac cgtttatcca tactgaagct gtttcatccc attgaagcag
     3001 cttttttggt tataccattg ccatatagga accggctgta ctaaaccaaa actgtatgta
     3061 atatggtaag gtatattatg tccttcaaag tgccggagaa cagccagcag actgttcaac
     3121 aggtagtcaa ccagtatttt aatgaattgc agcgaagtgg tcccggtggc atgcgaagtc
     3181 aatgctatgc gagccatgat gatcaatgca attttgtaca cattaaaagc ttcaaaaaag
     3241 aatctatcgc taatcatcat ttcaggtctg ctattttcag gcaatacctg gaaaagctgg
     3301 ctgttattgc cggtgagccg cttagtttct ccaggcttca ccagcaaaaa acatttgagt
     3361 cgatttatta atcgcctggt tgtttttgat aatgattaat gagcggattg ggtttataat
     3421 cttttatgat tttagacagt aaaacctgta aatttatccc gtcgaagcaa accaacggct
     3481 atgcacaggg ttattgtacc ggtagacttt tctgaaactt ctttgaatgc tgcccgctat
     3541 acagcgcaaa tgctggcagg taaggataat gcccttgccg tactttacca taattatgaa
     3601 agtcatgatg atcatgatgt atccctcaac taccaggaaa gcctgaaaaa agagttttta
     3661 aaggccgggg ttaaagccgt gcagacggaa agtgaaatgg gtggcgacct gattgaaaat
     3721 atttctcgcc tggcgcacac catccgggca agtttgattg taatgggtat taccggtaaa
     3781 tctgccatca gggccgccat gtttggaagt aataccctga aactaatcga caggaacctc
     3841 tacccagtca tgatcattcc accggatgca aaatacaagg gtattaagaa tgttgccttc
     3901 gcatcagatt ttaaaaatgt agaagaatca actcccgctg ttttgctgga ctctgttctt
     3961 gatctgttca aaccacagct tcatattttt catgtaaaca aggatatcag tgttagcgaa
     4021 tctgccgaag tagtcgggca gaaggcatta tttgagcaga tgttcaaaaa atatgatacc
     4081 cgttttcatt tccttattaa aaatgatttt tattctgcgg ttgacagttt tatcaaagac
     4141 tatgaaattg atgtgatgat aaccataccc aaacaccaaa gtaattccat gtccttattt
     4201 aaaaccacac ataccaagcg gttagcttat catagtcata tcccgatact ggctgctcat
     4261 gaataaggta ttaacagttt ctttccttgg gttttcggtg tatatttaat ttttaatacg
     4321 atcatctaaa caatgatttt atgaaaaccg taatagtacc ggtagatttt tcagaaacat
     4381 ctctcaacgc tgcccgttac gccacaaaat tgctgacagg ccactatggg gtaaccatgg
     4441 ttctgttcaa tttttgcgat aacgaaaaac aaatacctga agctattcaa agccttgaga
     4501 acttaagagc cttgctgcgg gaatcaggca ttgtcaaaac agagatactg gctgttacag
     4561 gccccggatt catttcggaa ctggacagaa tgtcccgtga agtagaggct gaccttgtca
     4621 taatgggcat aacaggtcgc tcccctattg gtcaaagcct cataggaagc aatacactca
     4681 aaatggtaga gaaaagaact tgtcccgtac tgatagtacc ctcatctgct acctacaaag
     4741 acgcaaaaga agtaatgctt acatcagact ttgataatgt tgagtttact atacctgcac
     4801 aacaaattaa aaatgtactg aaccggcttc gtcccaatct tcatataatg aatacggatg
     4861 aagaacatta tatctctatc accgaggaat ataaagccga aagggaaaaa ctggcttccc
     4921 tgtttgcaga ttttcaccct gaatttcatt tcctaacttt aaatgatgta caagatgcca
     4981 tttaccagtt tgcccaggac aaacaaatag acctcatcat tattgttcat agggagcaaa
     5041 cattgttttc aaagcttttt gtcaagagta atacgaaacg actggcctat tacagcacaa
     5101 tccctgtgtt ggccgttcat gaataaccgg ccggcaacag tgcttaaacc agtgccttga
     5161 catcattggc acaatttatt acctttactt taccatatgc gatttgatct gatccataca
     5221 tgctatatat tttcatttgc ggagcaattg ctgcctgcaa atttttatat ccctgattaa
     5281 tcacagtttt ttctaggctt acttactctc cccattactt ttcaggcttt tgcctaacct
     5341 aataatgcta tatgaagaat atcaaattga ttgaagtacc atccgaaatc ggtgccggca
     5401 caagaggagc cagtttgggt atagaagcga taaaaattgc agcgctggat ttcatgagca
     5461 atttttttat ccatttccct tctgaaaaaa ttccaacaga aaataagttg ctatacgaac
     5521 ctatcgaatc tccttatgct aaaagaataa aaggagtggc agctatatat gacagggtga
     5581 gcaaagcagt atgtgatacc cttaaaaata atttctttcc ggtcatttta agcggagacc
     5641 atagtactgc gggtgccacc attgccggaa taaaaatggc taaacctaaa agtaaattag
     5701 gtgtcatatg gattgatgcc cactccgacc tgcatacacc ttataccact ccttccggaa
     5761 atgtgcatgg tatgccgatg gcagtatcta ttaataagaa taatgaagaa tgtgccgttc
     5821 atgatgtaga tgatgaaact gccaaatact ggaactacct gaaaaatatt ggtaaaatag
     5881 caccaaaggt tttaccggag gacattgtat ttatttcgct gagagattac gaaaaggaag
     5941 aaagacatct gatcgaaaaa tacgacatga aagtgatcac caccaaggaa gtcagaagca
     6001 aaggtgcaga gaatgtagtt agatcagtac accgctacct tgccgattgt acagatattt
     6061 atatatcatt cgatgtggat agcctggatt cttcaatatc caaaggtacg ggcacccctg
     6121 tgagcaacgg cttaaaagaa agagaagcag aagacctgat cagtaagttt atgcaaaacc
     6181 ggaaagtatg ctgctttgaa attgctgaag taaatcccac tcttgataaa gaaaacctga
     6241 tggcggaaat agctttcaat attctgcaac gaagtgtgaa tgtgttaatg atgaattaat
     6301 caaccgggat gcaaagaaat tttctagtcg ccatattact tatttctgct ttcccatctt
     6361 tatcacagtt gggcagttat gtcgattcca taaataacta cagaagcaaa tatgtaaaag
     6421 agcatgaagt tgtaaaaggc agtgacaaaa agaatatgag cttttttcct gcagatgaaa
     6481 cctatcgtgt cattacccgg gttgaattaa ttgaagatgc accctggttc agtatggaaa
     6541 catcatcaga taaaaagaaa ccctaccggg tatatgccat acttcatttt aagctaaaca
     6601 atgaagcgtt taaactgcat gtttaccagt cgaaggatct attgaagatt gcagaatatg
     6661 cagatcatct gttcataccc tttattgatg agacttgtgg tgaagactca tacgaaaacg
     6721 gacggtatat tgacttaacc attaccgata tttcacaagg aacttatttc cttgatttca
     6781 ataaagcgta taacccctac tgtgcttata taagcaataa atacagttgc cctgttcctc
     6841 caaatgaaaa tcatctggct gttgccataa aagctggcga aaagaaattt ggagatcatt
     6901 gatgatgtgc ttatgtatca aaagtcttcc ttatgccttc atcaaaggaa accggatcat
     6961 aacctaattc ccttctcgcc ttttcaatat tgaacccggt ttttgccgga cgcattgccg
     7021 gctgcgagaa aatggccgct gttacttttt taatcagtaa tttattcaac cctaaataat
     7081 cagctgtttc acatgccatt tgatagggtg tcagtatatc tttcccggaa agatgaaata
     7141 ttccggttgc tctcctttgg atgatagcta caataccggc tgccaggtct tctacaaatg
     7201 tgggggtacg aacctggtca tcaaatacac tgtactcctc tcctttttcc agtttctccc
     7261 ttacaaccgt aaggatatta ccacgacccg taagtggttt tccgtacacc agcacggtac
     7321 gcactataga ccagttaaaa acatattgct gaaccagggc ttctgcccta agctttgtca
     7381 gtccgtagta attgacagga ttacccttgt cctcctctgt atacatcccc tccttaccat
     7441 caaaaatgaa atcagttgat atgaagatga aaaaactttg tacagcagcc gccgcttcca
     7501 aaaggtgccg ggttccatca atgttaactt tatctgcata gtctttgtgt tgttcacatt
     7561 catcaggctt gctgagggcg ccgctatgta cgatgacgtc aggtcttgaa tttcctatta
     7621 tgctctgaac ggcttccgga tcagtaaaat ctaatgattg gaattgaaac aatcgattat
     7681 ctgaataagg taagcggcac tcacctttgc ccgttgctat cacagcaatt cccttagtta
     7741 ataactgccc ggtaagatag tggcctaaga aaccattggc acctgtgatc agcactttca
     7801 tgatccaaaa gtaatgggaa aacaaaaagt cctgtcttta tttgacagga cttcttataa
     7861 attaggttga accgattaag gtttgttcag cgcttcaacc agtgttacaa tttctgattc
     7921 gctcttcaac ttcaggttat tgctggcagc gatctcttca attttactgg ccagtgccgg
     7981 caaagcttct gcaatatctt ttttagatgc tttgatcctt ttgatatctt ttcccggaat
     8041 atagagataa agctgctctg attttaccag ctccctgcgg ctgtaatcac ccagtgtgtt
     8101 ggcatcttcc actttctgtg aaatatattt aagcaaatgt gcattgggcc cggccgacag
     8161 aacctggtaa acggccctgt cggaatgctt gtcgatttcc gggtaaccca tggcaaattc
     8221 ataatgcttc agggtaccgt catcagcctt ttcatcccag gttaccgatt tgaaattatc
     8281 cagcttcaat tcctgcccgt catccatctt taccatcata aaattgttat agatgttgaa
     8341 cttaacggga atgtcggcaa catacttgtt atccttcagg tgaatcgttg cgtttttata
     8401 ttccgtagtc atgaagggag aaccggatac ttcctgcaat gcaggtatac gcattggaga
     8461 aactttcagg tcatcaattg ttgcctctgt ttcgggagat gcttgtccaa agagtgagga
     8521 tacagccatc aatgcaaatc caaataataa tcctttgctc atgtaattaa aaaatttagg
     8581 tgtgcaaata tattgcacac ctaaataaaa atttcaacca cttatccttt taacaaagca
     8641 aacaaccgtt tgctttagta tcttattaca tctttatttt atatctggtc aaaatccttt
     8701 tttctgaacc ggggccgatg gttccctgaa aggcattccc aggttataca ttacaaaact
     8761 ccagatatcg gtggcctcat ctatttgctt actggtaggc tttcctgcac catgacctgc
     8821 atttacatct actcttatca atacaggatt agcacctgaa tggtattcct gtaaacgggc
     8881 agcaaactta aatgagtggg ccggcactac cctgtcatca tggtcggctg tggtaactaa
     8941 cgtagccggg tagctgacgc cttgtttcag gttgtgatac ggtgaatatt tgtacaggta
     9001 tggaaactgc tttgcagaat ctgcattacc atactccacg gcccatcccc agcctacggt
     9061 aaacttgtga taacggagca tatccaatac acccaccgca ggcaaggcta ctttaaacaa
     9121 atctggacgc tgtgtcatga cagcgccaac aaggaggcct ccattgctgc cgccgcggat
     9181 agcaatcttt tgactgttgg tgtatttctc tttgatcagg tgttcggctg cgcctataaa
     9241 atcatcaaaa acattttgtt tgttttccag catacctgct ttatgccatt tctcaccata
     9301 ttcactgccg ccacgaagcg taacagatac ataaatacca ccctgctcaa taaagaaagc
     9361 attgctggta ctgaaacccg gtgtgacagg aatattgaag cctccgtagc catagagcag
     9421 taccgggttt tgcccgttct ttaccaggcc ttttttatag gtcaggaaca tgggtacttt
     9481 tgtgccatcc ttactggtaa agaaaacctg ctcggttaca aaatcatctg ttttaagctt
     9541 aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnagggaggt gtgctgaagg aagagaaggt atagaaaaac tccttgtctt
     9661 ctctttctcc gccaaaacca ccagctgagc caattcccgg taatttgatc tcccgtacca
     9721 ggtcaccatt gtaagtatac tggtacactt tggtggaagc atctttcagg tagctcgcaa
     9781 acatgtaccc accggcggta cctactccct gcagcgcttc ttctttctcc ggtattattg
     9841 ttttccagtt ttctttgccc gggtttttag gatcgatcaa tacgacttta taattggggg
     9901 ctccatcatt ggttcttacc agcagcctgt tgctgtaatt atcgatcaca tcaggttcag
     9961 tagtgaaccc tttgatcaac aggttgaatt tatcctgtgt ggggtnnnnn nnnnnaatcc
    10021 cagtaccaaa tttctgcacc actggtacct tctgaaacct ggaggattag aaatcgttta
    10081 tcctctgtaa gcccggcgct atgataccgg agcggatgtt ctttgtcctg gtagatcagt
    10141 tcatcctgtg actgatcagt gcccagcttg tgataaaata ctttatgatt ctgattctgc
    10201 ttgctgagct ttgttttttc atcaggctga tcatacccgc tgtaatagaa gccatcatca
    10261 cctttccagc taaaaccgct gaatttgatc cagcgtatct tgtctnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnttt ccggatttac tgaagctggt tgaacccaac gcagcaatac
    10441 cttctttgtt cagtgtattg gggttcagaa attcttccgg ctttccatcc agtcctttct
    10501 ggcgataaag cactgcctgg ttttgcaaac catcgttctt atagaagtaa tagtactcgt
    10561 tctttttgga aggagaagaa taccttggat agttccacag ttcttccagc ctttttttga
    10621 tcttgtcccg ctgaggcagg gtggcaaggt aatcaaaggt cactttgttt tgggcttgta
    10681 cccatgcttt agtctcttca ctgttatcat cttcgagcca gcggtaagga tcggccactt
    10741 tggtgccatg ataatcttca actgtttctc cttttttagt agtaggatac tgcaactggc
    10801 ctgctgccag taagggtgct ataaacatca ggaatgcaaa atgtttcttc atacaaatga
    10861 ttttactgct tctaagttaa ggcaaagcca ttgtaagcag caaaagctgt tcattaatta
    10921 gcctttgact tgacaggagg ccgtaaacgg ggataaatgc cttgtcagga tacctggttc
    10981 agcaataagg gcaaaccgga tttccattcg ccgtcaacat ataattcgaa ggaatagcgg
    11041 ccggcttttt caaactgcag ctggttgaag ttgatcacaa cgtttacaga tgcaaattgc
    11101 ccgttgggcg gattaccgat attgatattg ccttcaattg gctgaatgat ctcctttcca
    11161 tcggtgttga taagtcttaa cttaaaatca tgcgggccgg cttctttggc tgcaaagcga
    11221 aggcggcagg ccagcgaaca ggcaggatgc tgcgccggga attgtttgga attaatactg
    11281 tcaaaagtgc ccacaatggt aagcttggag ttattatcct gggcaaaatc cgccagggtg
    11341 aagatttcaa tttccataat cagtttttaa gaatgagtaa agctatgctt tgtttgattg
    11401 acttaatcca taccgaataa ccgtgccggg gctaaaaaag gcgctgccgg tgacaaaaca
    11461 ggtaactttt ggtcttcaat cctgacaggg cgggataaat cctgtcatcg aagcataaac
    11521 aaagctgcct gtactgaatt ttttaccagc atttccttac cgggaaaaag aaatactatt
    11581 cccgaaccat ggttattttt ctttgttttt ccagatcnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccgtttccgg gcagttgcaa tacaatggct
    11761 gaattgtcac cctcaatgga gatacgatta actgtactat tgtattaaaa acaagagatg
    11821 tggggataaa accccgggta agagctgcct acaggcatga ttatgtggtg gcagaattca
    11881 ataatgattc tatcaggatt atttatcccg gcagtgtcag ggcatatttt gtccggtggg
    11941 atgaaacaaa acctgaacaa gtgagctggt atgtatcaga tttcattgac gtcgcatatg
    12001 ctatgcttat taaacctgaa aaaacaaaat caagcaannn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnncattt atggtatttt gaacagcccg accccggctc caaaaatgac
    12121 cagtttttta tattggaaga aagcaacacc ggaaaacgaa agtattttac ttcgacccgc
    12181 tggctgaaga aacaactatc tgattggccc ggtatcaata caaagaacac cgcttataat
    12241 aactggttta ccggaaaaca agttatcgta aaagactaca accggtataa ggcagggcaa
    12301 taaacgcatt gggaataccg tcaaaggcga tagcataaat acaaactgca aaaaattacc
    12361 tggatgactt caaaaatgtc acggtcattt tctttaacaa aaactcacaa taatggtaac
    12421 agccatgcag acctttgttg gtacccggcg gataacctat accgagatgt acatcaaagt
    12481 ttgacccggg agttacaaat ccttcaaaac ttatagtacc gttttttctt gccccgattt
    12541 cctgcacatc tgttgttacg gaaacaggcg gtaacattat tcctgcagtt ttccacccgt
    12601 cagtaggtgt cgttataaga gtaccagacg ggtaaacaaa gtctggtttt attaaggagc
    12661 tgttaggtaa atcattaacc tgtgtactga caaatctatt acctgttctt aacattaact
    12721 ccattgctgt attggctcca taagtatccc acccggtata catgaacgaa tattcaagca
    12781 caatctttgc cttaatgatt tcgggatttt tgggaacggt gatgttattc ggataaccta
    12841 tagcaaaaac accctggtaa caatcttctg ctccatcaca tcgcctggag gtgaaactga
    12901 ttttgctggt agcagactca tgtgaataat tgacaggtct tggcatttcc cggttaggat
    12961 aattattgaa aacccataaa gcaaaaaaag acggaggcac tttttcaatt ttcgttacca
    13021 ccaggcctgc tgttgccaac gcttctctta ccaggtcacg gtcaagtatc agcttacctg
    13081 ctgtttttac acggttgtaa taagcggtaa gcggttgctg cacggatagt ttctttacag
    13141 ggttaatacc cgccttagta tacgactctg ttaaaagcgg tttattcgat gaaaaaagat
    13201 ttttcacttt tgaaaaattc gttgtcgaat ccttcgcggc atcaataaga tctgaaattt
    13261 tttggcgtaa ttgcagatac ccggtattag tttttgcagc tgtcttaaac tgatcaaatt
    13321 cctttttcca gttgtcctgt gctgaaatgg atagtgacag accaaaaata aaagctaaaa
    13381 atataatccg ctacatgtga tttacttttt ataaatatac ttctaacgcc gatgaaatga
    13441 aaagaaaaat attgattatc tatattttga accggctgaa cctgcccaac catggaaaat
    13501 tt
//
LOCUS       DAORLS010000050        13039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2560_length_13039_cov_1.953943, whole genome shotgun sequence.
ACCESSION   DAORLS010000050 DAORLS010000000
VERSION     DAORLS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2560_length_13039_cov_1.953943"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>847)
                     /locus_tag="PK977_05745"
     CDS             complement(<1..>847)
                     /locus_tag="PK977_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17648.1"
                     /translation="HTTLPFVIHDTIAATLSAVQCEGYFSIDWNSIPGATGYEIFRLL
                     GDEMVPVATVSAATLTYTFSGLSKDSVYWASVRPIIGSTPGRRAIAVSRKPDNGNCSG
                     SISDNDIKIDSILSPNRSGRLLTSTALGNSVPVTIRIKNLDDQSTSGNIDVSYSLNGG
                     PVVTETINNPAIAPGSTLTHSFSAINNIDLSAANTYILEVTVAPQTPDPVTANNSQIF
                     ICKQLPNPSITGATPAVPFIDNFDAVSVQSYTSRQTGLLGLDRYDFVNSNENGRIRTF
                     INSGIA"
     assembly_gap    848..859
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<860..3064)
                     /locus_tag="PK977_05750"
     CDS             complement(<860..3064)
                     /locus_tag="PK977_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF17649.1"
                     /translation="MRFYPIPLMAFVLLLSLSVSAQDDSRFSLLLKSGTIVPPKNITV
                     ENIDNYNRVASRTNGKALAVIQFEQIPSPSERLQLKQSGIELLDYVPNNAYTATITGS
                     LDLFHLLDFKVRSVVELTPVQKMQPELAAGIFPGHAVKVAGTVDVWISFPSSYAFEEV
                     REQFNKKNIDVIGSRLKEYRILELRIASGRLTELASFPFVEYVQVAPGEDKPLSDLWT
                     NWGIDGIRAKFLNAPLSVGGENLKGNGVTVGVGDDADFQQHIDFTDRLISRAAAPYNF
                     HGTHVGGIVGGAGIRKELRAGFAPKSTIISQVFSNVFTNAAAYTADHDMVITNNSYGN
                     ILNDCNTFGFYDLYSRTLDQQAFAYPSLQNVFAAGNSGEYKCAPFPDSFRTIIGGYQS
                     AKNVITVGNFTPIGYLYQQSSKGPVRDGRIKPEIVTIGSFITSTAPPPFNYYWENTGT
                     SMASPAMSGGLALLYEKYKLLHGVNPKNGLMKALVCNSGDDYGNPGPDYSHGFGLANF
                     QRALDMLKNNRYQSNSITPGPPQTININAPSGLAEIKVMLYWNDPAAAAVASQTLVNN
                     LDLEVVDPSSNIILPLILDTAYVNIKNNAVQGVDNINNIEQVVIKNPAPGNYIIRVKP
                     TNITQNPSQEYFVVYDFVPVETKITTPVGGETFIHGENVVVRWDSYGDPQNQFTLEFS
                     SDDGATWSTLKNNIASNRRDYFYTAPAPNEWFVVPQVTTDKARMRITRNSTTI"
     gene            complement(3183..6611)
                     /gene="mfd"
                     /locus_tag="PK977_05755"
     CDS             complement(3183..6611)
                     /gene="mfd"
                     /locus_tag="PK977_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRF17650.1"
                     /translation="MSVKELLERYQNTPRLFQLADKLSFAPAQKIYLENLQGSSSQFI
                     VAAAFTHPSLSQLNHLIILNDAEEAAYFHNTLENLTEALDIFYFPSSFKNRKNYQLLN
                     SSHVMLRTEALTKIAAGGNKKVLVTYPEALFEKVVLPGTLSSNIIHIKTGDTINTEQL
                     LLKLADYGFERTDFVYEPGQFAIRGGILDIYSFGNEKPYRVELFGNEVDSIRIVDPET
                     QLSERKLLQVSIIPNVDTQFKSEDRISLFEFLPENTVVWIQDYELCKEHIADSEEDLN
                     GFLRLQEEIKNQPSRSRKEEADENLSKKDVRTDDFISVTEFEKKMFERHVVAFGHKDS
                     FPVHYPAFTIQHHTKEQPSFNRQFDLLIRDLKGWEAKKYQLFIFAENPRQLERLQSIF
                     DDLKAEIVFNPIATSVHEGFIDEDLQVVCYTDHQVFQRYHKYKVKQAYNKNKALTLRT
                     LRELQPGDFVTHIDHGVGIFSGLQKLEVNGRLQEAVRLIYKDTDILYVNINSLHKIAK
                     YTGKDGSVPKVNKLGSDVWNKLKEKTKTKVKEIAFDLIKLYAKRKAQQGFAHTPDNYM
                     QTELEASFIYEDTPDQSKATADVKKDMESPSPMDRLVCGDVGFGKTEVAIRAAFKTCV
                     DGKQAAVLVPTTILAFQHYKTFKDRLKDFPVTVDYLNRFKSSKEKKETLKKLEEGKID
                     IIIGTHGLLGKEVKFKDLGLLVIDEEQKFGVGHKEKIKTLRTTVDCLTLTATPIPRTL
                     QFSLMGARDLSIMNTPPPNRQPIQTEVQVYNEDFIRDAIYFETERGGQVFFIYNRIAG
                     LAEMASIIQALCPDLSIGYAHGQMEGHELEERILDFIERKYDVLVCTNIVESGVDIPN
                     VNTIIVNNAHQFGLSDLHQLRGRVGRSNKKAFCYLLAPPMTTLPNDSRKRLQTLEQHS
                     DLGSGFQIAMRDLDIRGAGNLLGGEQSGFMAEIGFEMYQKILDEAIRELKRTDFKELF
                     KEEIAKQDDFVQDCTIDTDLEILIPDEYVESITERLSLYQRLDNCETEEELIAMKQEL
                     GDRFGPVPQQVEDLFITVRCRKLAVDLGFDKMSLKDDTLRCFFINRPDSPYFESETFQ
                     KIIQFLQTGTNKGKLKQTGKLFLLIASDIKNMDALYRFLRTMHQFCFTNEAVTV"
     gene            6717..>7289
                     /locus_tag="PK977_05760"
     CDS             6717..>7289
                     /locus_tag="PK977_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF17651.1"
                     /translation="MALLPWRKGIVTKIDDHTHNTRRFWIQVPELEKFDFVPGQFVTL
                     DLPIHEKPNKRWRSYSIASWPDDSNTFELLIVLAQGGLGTSYLFDFKNISVGSELTLR
                     GPQGVFTLPEKLDKDLFLICTGTGIAPFRSMVNYIKLKKISFKNIHLVFGCRKKEDLL
                     YYEELKKLELELPGFHYHPTLSREHWDGHYG"
     assembly_gap    7290..7302
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7558..7908
                     /locus_tag="PK977_05765"
     CDS             7558..7908
                     /locus_tag="PK977_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRF17652.1"
                     /translation="MGASSLIVLIIAAIAFSGIAILLSKFVLKASTNKQKGEPYECGI
                     PTTGKTWIQLNVGYYLFALIFLIFDVELVFLYPWAVVAKSMGWIALIEIVIFFFILFM
                     GFLYAHKKGALKWM"
     gene            7950..8528
                     /locus_tag="PK977_05770"
     CDS             7950..8528
                     /locus_tag="PK977_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRF17653.1"
                     /translation="MSDEVKISEDKMILPQRPEKLEDTFPGQVHNLAGGEVVLSKLDD
                     LINWARSNSIWPLTFATSCCGIEYMAVAGSKYDFSRFGFEVARATPRQADVIIIAGTI
                     VNKMAPVLKRLYDQMADPKYVIAMGACAISGGPFFYNTYSVVKGADHVIPVDVYVAGC
                     PPRPEALLHALITLQQKIKSGLTREQIRTGIE"
     gene            8528..>8761
                     /locus_tag="PK977_05775"
     CDS             8528..>8761
                     /locus_tag="PK977_05775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRF17654.1"
                     /translation="MTNEELKIKITELLPEATFDESGEWITVNMEPQSWLSFARQLRS
                     DDTLFFDYLFCLTCIDWKTHLTMVYHLSSTRYRH"
     assembly_gap    8762..8830
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8990..10119
                     /locus_tag="PK977_05780"
                     /pseudo
     CDS             8990..10119
                     /locus_tag="PK977_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
     gene            10109..11197
                     /gene="nuoH"
                     /locus_tag="PK977_05785"
     CDS             10109..11197
                     /gene="nuoH"
                     /locus_tag="PK977_05785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRF17655.1"
                     /translation="MTDNMWSFRHIANEIHAWLSATFNPTWTLIIEMVLAGVAVIGLF
                     AALGLVLVLMERRVAAWIQIRLGPNRVGPKGLLQSLADTLKLIVKEGLTPDGADKLMF
                     NIAPFIAMIVAMLLMAPIAFAKDFQLWDLNIGVLFVSAVSSLMVISILMAGWSSNNKY
                     SLLGAMRSGAQIVSYELSAGLSILTIVVFTGSLQVSDIIHAQANGWWIFKGHLPVWIA
                     FVIFIIAVTAETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYINVFIVCAIGA
                     TLFLGGWMPFHIGNWEGFNHVMDYIPSSVWFFGKTFFLIFVIMWFRWTFPRLRIDQLL
                     NLEWKYLLPISMINLLVATAMAILGWHF"
     gene            11262..11738
                     /locus_tag="PK977_05790"
     CDS             11262..11738
                     /locus_tag="PK977_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF17656.1"
                     /translation="MFITKYIKEVFGAVKSLFTGMKRTMYYFTHHKEIITQEYPDNRD
                     KLVLPERFKGEVVMPHNENNEHRCTGCQACELACPNGTIKVIFKNELTPEGKKKKAID
                     KLVYHLELCTMCNLCVEACPSDAIKMAQTFEHSVYDRSHLTKILNKPGSKIMEGVE"
     assembly_gap    11870..11924
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11925..12257
                     /locus_tag="PK977_05795"
     CDS             <11925..12257
                     /locus_tag="PK977_05795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRF17657.1"
                     /translation="AGGIAVLIIFSIFLTQQAGEKLPKQKIGRKIFSALAVFCAFALT
                     MVQVYQHEFSKIITDAAPATVRNIGEKMLSTEQGGYALPFEVISMLLLAAMVGCIVIA
                     LRSKPETK"
     gene            12254..12571
                     /gene="nuoK"
                     /locus_tag="PK977_05800"
     CDS             12254..12571
                     /gene="nuoK"
                     /locus_tag="PK977_05800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRF17658.1"
                     /translation="MTIEPGLYHLLFISTALFFIGVYGFFTRRNLITMLMSIELILNS
                     VNLNFIAFDKYLWPGKMDGLFFTVFIIAIAAAEAAVAIAIIINLYRSHQSIDVENAED
                     LKY"
     gene            12578..>13039
                     /locus_tag="PK977_05805"
     CDS             12578..>13039
                     /locus_tag="PK977_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRF17659.1"
                     /translation="MPNATIIALIPLLPLAGFLLLGLFGRKYLKTSSGIIGTALLLTS
                     TILALYTAYEYFFVYGKVNGIYQQLTPLKITWLEFSKGVSIDMGLMIDPISVMMLVVV
                     TLVSLMVHIYSIGYMKGEERFSTYYAFLGLFTFSMLGLVLSTNIFQIYIFWE"
BASE COUNT         3696 a         2766 c         2650 g         3778 t
ORIGIN      
        1 agcaattcct gaattaataa acgttctgat tcttccgttt tcattgctgt taacaaaatc
       61 atatcggtca agtcccagta accctgtttg ccttgatgta tatgactgaa cggatacagc
      121 atcaaaattg tcaatgaatg gaacagcagg tgttgcacct gtgatggatg gattaggcag
      181 ttgcttacat atgaaaatct gagaattatt tgcagttacc gggtcgggtg tctgaggtgc
      241 tactgttact tccaaaatat aagtattagc agctgataaa tcaatattat taatagcgct
      301 aaagctgtgt gttaaagttg atccaggagc tattgcagga ttattgattg tttctgttac
      361 aacaggaccg ccgttaagtg aatagcttac atcaatattt ccactggttg actgatcatc
      421 caaatttttg atcctgatag taacaggtac actgttgcca agtgcagttg aagtaagcaa
      481 cctgcccgat cgattagggg aaagaatact atcaatctta atatcattat cggaaatact
      541 accagaacag ttaccattat caggcttacg agaaacagcg atggctcttc ttcccggagt
      601 gcttccaata atgggacgaa cactggccca ataaaccgaa tctttactta agccactgaa
      661 tgtataggtt aatgtagctg ctgaaacagt agccactggt accatttcat caccaagcaa
      721 ccggaaaatt tcataacctg ttgccccggg tatgctgttc cagtcaatgg aaaaataacc
      781 ttcgcactgt acagcagaaa gtgtggctgc tatagtatca tgaattacaa atggtaaagt
      841 agtatggnnn nnnnnnnnna atagtggtgc tattacgggt tatccgcatt ctagctttgt
      901 ctgttgtaac ctgtggtact acaaaccatt catttggagc aggagcggta taaaaataat
      961 cacgtcgatt agaagcaata ttatttttta aagtactcca ggtggctcca tcatctgagg
     1021 aaaactccaa tgtaaactgg ttttgcgggt ctccataaga atcccatctc accactacat
     1081 tctcaccatg aataaatgtt tcccctccaa caggtgtcgt aatctttgtt tcaactggta
     1141 caaaatcata cactacaaaa tactcttggg aggggttttg ggtaatatta gtaggtttaa
     1201 cacgaattat ataattaccg ggagccgggt tttttattac aacctgttct atgttgttaa
     1261 tgttatcaac tccctgcaca gcgttgttct taatatttac ataagccgta tcaagtatca
     1321 atggtaaaat gatattggat gaagggtcaa caacttccag atcaagattg tttactaacg
     1381 tttgggaagc tactgcagct gcagcagggt cattccagta caacataact tttatttcgg
     1441 ccaaaccaga aggagcattt atattgatag tttgtggcgg tccgggagtt atgctattac
     1501 tttggtaccg gttattcttc aacatatcca atgctctttg gaaattagca agcccaaagc
     1561 cgtgagaata atcgggaccc gggttaccat aatcatcacc actgttgcac accagcgctt
     1621 tcatcaaacc gttcttggga ttaacaccat gcaacagttt gtacttttca tatagcaaag
     1681 ccaacccccc tgacatagca ggtgaagcca tgctggttcc ggtattttcc caataataat
     1741 taaatggagg cggagcagta gatgttataa aagaaccaat agtaacaatt tccggtttaa
     1801 ttcgtccgtc acgaacaggt cccttgcttg actgctggta cagataacca atcggtgtaa
     1861 agttccctac agttatgaca ttttttgccg actggtatcc accaattatt gttctaaaac
     1921 tatcaggaaa aggagcacat ttatattcgc cgctattacc ggcagcaaat acattttgca
     1981 aacttgggta ggcaaaagct tgctgatcga gagttcgaga atacaaatca taaaaaccaa
     2041 aagtgttaca atcattgaga atattgccat aagaattatt ggtgatcacc atatcatgat
     2101 ccgctgtata agcagctgcg tttgtaaaca cattggagaa aacttgcgaa atgatagttg
     2161 actttggtgc aaaacctgct cttaattctt tgcgtatacc ggctcctcct actataccac
     2221 ctacatgagt tccatgaaaa ttatatggtg ctgctgccct gctaatcaaa cggtctgtaa
     2281 aatctatgtg ctgctgaaaa tctgcatcat cgcctacacc tactgtcact ccgttacctt
     2341 taaggttttc tccgccaaca ctcaaaggag catttagaaa tttagctctt ataccatcta
     2401 tgccccaatt agtccaaagg tcactcaagg gtttatcttc tcccggagct acctgcacat
     2461 attctacaaa tggaaaagaa gcaagttctg tcagccttcc ggatgcaatc cttaattcca
     2521 gtattctgta ctcttttagc cggctgccga taacatcaat gttttttttg ttgaactgtt
     2581 ctctcacctc ttcaaaagca taactggaag gaaaacttat ccaaacatct acagttcctg
     2641 caacttttac tgcatgacca gggaatattc ctgcagccag ctctggttgc attttctgta
     2701 ccggtgtcag ttctacaaca gaccttacct tgaaatctag tagatgaaac aggtccagcg
     2761 aaccagttat cgttgctgta taagcattat ttggaacata atccagcaac tctataccag
     2821 attgtttcag ttgcagcctc tccgatggtg aggggatctg ctcgaactgt ataacagcca
     2881 aagcctttcc attcgtccgg gaggcaaccc tgttgtaatt atcaatattt tctaccgtaa
     2941 tatttttggg aggaacaatc gttccgcttt ttaataacaa actgaagcgg gaatcatctt
     3001 gtgccgagac agacagagac aacaaaagca caaaagccat caagggaatt ggatagaatc
     3061 tcatagtgat tttagtaata ttaaaagtaa gataattagc cgagaaaagc caatcctgtt
     3121 aagcccctga ttaacatacg ttacaaagca taaaaatggt tgcctcctga agcaaaatgg
     3181 cactaaacag taactgcttc atttgtaaaa cagaattggt gcattgtcct tagaaaccgg
     3241 tacaaggcat ccatattttt aatatctgaa gcgatcagca aaaagagttt gcctgtttgt
     3301 ttcagttttc ctttattggt gcctgtctgc aagaactgaa tgattttctg gaatgtttcc
     3361 gactcaaaat agggcgaatc tggccggtta ataaagaaac aacgaagtgt atcatctttt
     3421 aaactcattt tatcgaaacc cagatccact gccagcttac ggcaccttac ggtaataaaa
     3481 agatcttcta cctgctgcgg caccggccca aaacgatcgc cgagctcctg tttcatcgct
     3541 atcagctctt cctccgtctc acaattatcc agtcgctgat aaagcgacaa tcgttctgta
     3601 atactttcaa catattcatc cggtatcaaa atttccaggt cagtatcaat agtacaatcc
     3661 tgtacaaaat catcctgctt tgcaatttct tctttgaaaa gctccttgaa atcggttctt
     3721 ttcagttcac gaatagcttc atccaatatt ttttgataca tctcaaaacc aatctctgcc
     3781 atgaagccgc tctgctcacc gcctaaaagg tttccagctc cccgtatgtc caggtctctc
     3841 atagcaattt ggaaaccact gcctaaatca ctatgttgtt ctaatgtctg cagccttttc
     3901 ctggagtcat tgggcaatgt agtcattggt ggtgccagca aatagcaaaa ggctttctta
     3961 ttactgcggc ctaccctgcc tcttaactgg tgcagatcac tgagaccaaa ctgatgcgca
     4021 ttattgacaa taatcgtatt cacattagga atatctaccc cactttctac aatgttcgta
     4081 cagaccaata catcatactt tctctctata aaatcaagaa tcctttcctc cagttcatgt
     4141 ccttccattt gtccgtgagc ataaccaatg ctcaggtcgg ggcaaagcgc ctgaataata
     4201 gatgccattt ctgccaggcc ggcaatacga ttgtaaataa agaatacctg gccgcctctc
     4261 tcagtctcaa aataaattgc atcacggata aaatcttcgt tatagacctg tacttcggtc
     4321 tggataggct gccggttggg cggcggcgta ttcatgatac tcaggtctct tgcccccatt
     4381 aatgagaatt gcagggttct tggtatgggc gtggcagtca aagtcaaaca atcaactgta
     4441 gtgcgaagtg tttttatctt ttccttgtgc ccgactccaa acttctgttc ttcatcaata
     4501 acaagcagac caagatcttt aaacttcact tcttttccca acagcccatg tgtacctatg
     4561 atgatatcga ttttaccctc ctccagcttc ttgagtgttt ctttcttttc ttttgaagat
     4621 ttaaagcggt tcaggtaatc aactgtaacc gggaaatcct ttaacctgtc tttgaaagtc
     4681 ttataatgct gaaacgcaag tatggttgta ggaacaagca ccgctgcctg ctttccatcc
     4741 acacaagttt tgaaggcggc cctgatcgcc acttctgttt ttccaaatcc aacatcgcca
     4801 caaactaacc tgtccatagg agaaggcgac tccatatcct tttttacatc tgcagttgcc
     4861 ttgctctgat caggggtatc ttcataaatg aatgaagcct ccaattcggt ttgcatgtaa
     4921 ttatccggcg tatgtgcaaa tccctgctgc gcctttcttt ttgcatataa cttaataaga
     4981 tcaaatgcaa tctccttaac cttagtttta gttttttctt ttagcttatt ccatacatca
     5041 cttcccagtt tatttacctt gggcacactg ccatcctttc cggtatactt ggcaattttg
     5101 tgcaggctgt tgatattgac atataaaata tcggtatcct tgtaaattaa ccgtaccgct
     5161 tcttgtaaac ggccatttac ctccagtttc tgcaggccgc tgaaaatacc tacgccatga
     5221 tcaatatggg tgacaaaatc acccggctgt aactcacgaa gtgttcgcag ggtgagcgcc
     5281 ttgttcttat tatatgcctg cttgacctta tacttatgat aacgctgaaa gacctggtga
     5341 tctgtatagc aaacaacctg aaggtcttca tcaataaaac cttcgtgtac agaagtagct
     5401 atcgggttaa atacaatctc cgccttcaaa tcgtcaaaaa tactttgcag cctttccaac
     5461 tgacgggggt tttcggcaaa aatgaacaac tggtattttt ttgcctccca gcctttcagg
     5521 tccctgatca gcaaatcaaa ctggcggttg aaggaaggtt gttcttttgt atgatgctga
     5581 atggtgaaag cagggtaatg gacgggaaaa ctgtctttgt gcccaaatgc tactacatgc
     5641 cgttcaaaca tctttttctc aaattcagtc acagatatga aatcatctgt tctaacatct
     5701 ttctttgaca ggttttcatc cgcctcctct tttcttgacc tggatggctg atttttaatt
     5761 tcctcctgca gccttaaaaa gccgttcaag tcctcttcac tgtctgcgat atgctcttta
     5821 catagttcat aatcctgtat ccatacaact gtgttctccg gcaaaaattc aaacaaggaa
     5881 atccggtcct cacttttaaa ttgggtatct acattaggga taatgcttac ctgtagtaat
     5941 tttcgttcac ttaactgtgt ttccggatca acaattcgga tggaatcaac ttcgtttcca
     6001 aacaattcta cccggtaagg tttttcatta ccaaatgaat aaatgtccaa aatccctccc
     6061 cttatggcaa attggcccgg ctcatagaca aaatccgttc tttcaaaacc ataatcagcc
     6121 agtttcaata acagctgttc tgtattaatc gtatctcctg tctttatatg tattatattg
     6181 gatgaaaggg taccaggcaa tactactttt tcaaacaagg cttccggata ggtaactagc
     6241 actttcttat tccctcctgc cgctattttg gtcaatgcct ctgttctcag cattacatgc
     6301 gatgaattca aaagctggta attcttcctg tttttaaagg aagatggaaa ataaaatatg
     6361 tccagggctt cggtcaggtt ttccagggta ttatgaaaat aagccgcctc ctctgcatcg
     6421 ttcaaaatga tcaggtgatt caattgtgat aaggaaggat gagtaaatgc agctgctaca
     6481 ataaactggg aagaacttcc ctgcaggttt tcgaggtata ttttttgggc aggggcaaaa
     6541 gaaagcttgt ccgccagttg aaaaaggcgg ggtgtgtttt gataccgttc cagcagttcc
     6601 ttcacactca tgagaggcgc aaaattaatt gattggcaac gattgcagta ataaattcca
     6661 ataatcacaa cattcgcatg gccagatggt tttaccttta tcaaaaccag caaaaaatgg
     6721 cattgttacc ctggcgcaaa ggcattgtga ccaaaattga tgatcacaca cataacacaa
     6781 gaaggttctg gatacaggtg cctgagttag aaaaatttga ttttgtaccg ggccagtttg
     6841 ttacccttga cctgcctatt catgaaaaac caaacaaaag gtggcggagt tattccatcg
     6901 cttcctggcc ggatgacagc aatacatttg aattactgat tgtactggcg cagggcggat
     6961 tgggtactag ttatcttttc gattttaaaa atatttccgt gggcagcgaa ttaaccctcc
     7021 gcgggccaca aggagtgttt accctgcctg agaaattgga taaagacctt ttcttaatat
     7081 gcaccggaac aggaatagcc ccattcagga gcatggtaaa ttatattaag ctaaaaaaaa
     7141 tatcatttaa aaacattcac ctggtatttg gctgccgcaa aaaagaagac cttttatact
     7201 atgaagaatt aaaaaaactg gaattggaac ttccgggctt tcattatcac cccactttat
     7261 caagagaaca ctgggacggt cactatggtn nnnnnnnnnn nnatttatat ggacctctgt
     7321 aaaaacaggc aagaagcact gttcttcctc tgcggctgga agcacatgat tgatgaggca
     7381 aaaaaacata tcacagaaat gggatatgac aaaaaatcca tccacctgga attgtacggg
     7441 taacctctaa tggtaattca atattttttc aactgataaa aaacaaggct atcgttgata
     7501 actgtcatgt tcagttttta acgcatagtt cctatattcg tgcaaaccaa tttgcgtatg
     7561 ggggcttcct cgcttattgt actgatcatc gctgccattg ccttctccgg cattgcgatt
     7621 ctcctgtcaa aatttgtttt aaaagcatcc accaataagc aaaagggcga accttatgag
     7681 tgtggtattc ccacaaccgg caaaacatgg atacagctaa atgtaggtta ctaccttttt
     7741 gccctcatct tcctgatttt tgatgtggag cttgtttttc tatacccctg ggctgttgta
     7801 gccaaaagca tgggatggat cgcattaatt gaaatagtta ttttcttttt tatacttttc
     7861 atgggattcc tttatgcaca caaaaaagga gccctgaaat ggatgtaatg ccagttttaa
     7921 gaaaactaaa ccgatgatta atttcgaaaa tgagtgatga agtaaaaata tctgaagaca
     7981 aaatgatatt gccacaaaga ccagagaaac ttgaggatac ttttcccggg caggtgcata
     8041 accttgctgg cggagaggta gtccttagca aactggatga cctgatcaac tgggcaaggt
     8101 ccaactccat ctggccactc acttttgcca ccagctgctg cggaattgag tatatggcag
     8161 tagcgggttc taaatatgat ttttcacgat ttggctttga agtggcccgg gccacaccgc
     8221 ggcaggctga tgtaattata atagctggta caatcgtcaa caaaatggcg ccagtattaa
     8281 aaaggttata tgaccagatg gccgacccca aatatgtaat tgccatgggc gcctgtgcca
     8341 tcagcggcgg acctttcttt tataacacat attcggtagt aaaaggtgcc gaccatgtga
     8401 ttcctgtaga tgtgtatgta gccggttgcc ctccaaggcc cgaagctttg ttgcatgcat
     8461 tgataacatt gcagcaaaag atcaagagtg gtttaacaag agagcaaata agaacaggca
     8521 tagaataatg accaacgaag aactgaaaat aaagatcacc gaattgctgc cggaagcgac
     8581 ttttgatgaa agtggcgaat ggatcactgt gaatatggag ccacaaagct ggttgtcttt
     8641 tgcccggcaa ttacgtagtg atgacacttt attcttcgat tatcttttct gcctgacctg
     8701 tattgactgg aaaacacatc tcaccatggt ataccacctg tcatctaccc gttacaggca
     8761 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn gtacagccga gtttcatgaa agagaagtat atgaaatgtt cggagtaaat
     8881 tttcttaacc atcccgattt gcgtttattg attttgcccg atggatggga aggaaagaat
     8941 ccgctaagaa aagattttga agatcccatt aatatgataa ggctttgata tgtacgaaga
     9001 agtaggtaca accactgaag gggacctggt cattaacgta ggaccacaac acccggccac
     9061 tcacggcgta ttgcatcttg tgattacgct gaacggggaa actatcaaaa aagtggaacc
     9121 tcatcttggt tatattcatc gctctattga aaaaatgtgt gagagcctca cctaccggca
     9181 atttatatat gttaccagcc ggatggatta tctctcttct catataaata accatgcctg
     9241 tgctttgtgt gttgaaaaag gcttacaggt ggaaatccct cccagggcac aagtcatccg
     9301 catattgatg gatgagctga caaggattgc gtcgcatgaa ttatggtggg gagccatggc
     9361 tatggattta ggcgctttta cacctttctt ccatgcgttc agagaaagag aaagtattaa
     9421 tgatatcatg gaagaaacct gcggagccag actgaccatg aattatatgg tacccggtgg
     9481 tgtgatgcag gacatccatc ccaattttca gaaaagagta aaagattttc ttgtacagta
     9541 caaaaagaag atcagggaat atgatgaact ggtaaccggc aatatcattt tccagaaccg
     9601 gatgaaggga atcggctact tgtcacctga ggatgccatc tcttacggct gcaccgggcc
     9661 cacagcgagg gggagcggag taagttcaga tatcagaaag atctatcctt atgaaattta
     9721 tgacaagctt gaatttgacg aggtgctgga aaccgcaggt gattcatttg cccggtacat
     9781 ggtacgcatc cgggaaatgc agcaatctat ccgcatcatc gaacaattaa tagataatat
     9841 acctgagggt gacttccagg caaaaacaaa agcggtactg aaacccccca attgaaatta
     9901 cctaaaggag agttttatac aagagtagaa actgccagag gcgaactggg agtgtatatc
     9961 gtaagcgaag gcggcactac gccttatcgc atcaaattcc gttcccccgg cttttcaaac
    10021 ctttctgtac tcgatcatat tgcacggggt tccaaaattg gcgacctcgt ggccatgatg
    10081 ggaacactgg atttagtaat acctgatatt gacagataac atgtggagtt ttagacatat
    10141 tgccaatgag attcatgcct ggttgagtgc caccttcaac cccacatgga cattgatcat
    10201 agaaatggta ctggccggtg tggccgtgat tggcctgttt gctgcattgg gcctggtatt
    10261 ggtattgatg gaacgaagag tagccgcctg gatacaaatc cgccttggcc ctaacagggt
    10321 tggccctaaa ggcttattgc aatcattggc cgacacgttg aaactgatcg taaaagaagg
    10381 actaacccca gatggtgcag ataaattaat gttcaatatt gcgccgttca ttgcgatgat
    10441 agtagccatg ctgctgatgg cgcccatcgc ttttgccaaa gattttcagc tatgggacct
    10501 caatatcggg gtattatttg tgtctgcagt ttcctcatta atggtgataa gcattttgat
    10561 ggccggttgg tcaagcaata acaagtattc cttgttaggc gctatgcgaa gcggtgcaca
    10621 aatcgtaagc tatgaattat ctgcaggact gtcaattctg acgatcgttg tatttaccgg
    10681 cagtttgcag gtttcagata ttattcatgc gcaggccaat ggctggtgga tattcaaagg
    10741 ccacctgcct gtttggatag catttgtcat tttcattatc gcagttacag ctgaaacaaa
    10801 ccgtgccccc tttgacctgg cagaagcaga gtcagaactg accgccggtt tccatacaga
    10861 atattcgggg atgaagtttg ccttgttctt cctggcagaa tatatcaatg tgtttattgt
    10921 ttgtgcaatt ggtgctacat tgttcctggg tggctggatg ccttttcata tcggtaactg
    10981 ggaaggtttt aatcatgtga tggattatat accatccagt gtttggtttt ttggaaaaac
    11041 gttcttctta atatttgtca taatgtggtt caggtggaca ttcccccgtt tgcggattga
    11101 ccagttgctg aacctggaat ggaaatacct gttaccgatc agtatgatca acctgctggt
    11161 ggcaacagca atggcaatat tgggatggca tttttagtga atgatgaata gtgagtggca
    11221 gatgatgttt tcttagtgtt aggcttatta gtctgaagta tatgtttatt acaaaatata
    11281 taaaagaagt ttttggtgca gtgaaatccc tgtttaccgg gatgaaacgg acaatgtact
    11341 atttcacgca tcacaaagaa ataattaccc aggaatatcc tgacaaccgg gacaaactgg
    11401 tgttgccgga aagattcaag ggtgaagtgg tgatgccgca caatgaaaac aacgaacacc
    11461 gctgcacggg ttgccaggct tgtgaactgg cctgcccgaa cggtacgatc aaagttattt
    11521 ttaaaaacga actcacacca gaaggcaaaa agaagaaagc gattgataaa ctggtttatc
    11581 acctggaatt atgcactatg tgcaatctct gcgtggaggc ctgtccttct gatgccatca
    11641 agatggccca gacattcgaa cacagtgtgt acgacagaag ccatctgaca aaaattttga
    11701 ataaacccgg atcaaaaatc atggaaggag tagaatgatg agcggttcca ccatattgtt
    11761 ttatttgctg gcagcattaa cattaggctg tggtatatta tccgtcagca ccaggcagat
    11821 attccgtgcc gctatttacc ttttgttctc gctgataggg attgccggtn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccggg ggcattgcag
    11941 tgctgatcat cttctccatc tttcttaccc agcaagccgg tgaaaagctg ccaaaacaaa
    12001 aaatcggaag aaaaatattt tcggcattgg ctgtattctg tgcatttgcc ttaacgatgg
    12061 tgcaggtata ccagcatgaa ttttcaaaaa taattactga tgccgctccc gctactgtaa
    12121 gaaacatagg cgaaaagatg ctgagcactg aacaaggtgg ctacgcattg ccttttgaag
    12181 ttatatccat gctgttactg gcagccatgg tcggctgtat cgtaattgcg ttaagaagta
    12241 aaccggaaac aaaatgacca tcgaacccgg attatatcat ttattgttta tcagcacagc
    12301 gctgtttttc attggtgtct acggtttttt cacccgaaga aacctgatta ccatgctcat
    12361 gagcatagaa ctgatcctga acagtgtgaa cctgaatttt atagcttttg acaaatacct
    12421 gtggcccggt aaaatggacg gactgttctt taccgtattc atcattgcta ttgcagccgc
    12481 cgaagctgcg gtggcaatag ctattattat taacctgtac cgcagccacc agtccattga
    12541 tgtcgaaaac gcagaagacc taaagtatta aacaattatg ccgaacgcaa ccattatagc
    12601 actgattccc ctgttgccgc ttgccggttt cctgttgtta ggtttatttg gcagaaaata
    12661 tttgaaaacc agttccggca ttatcggaac agccctctta cttacatcca ccattttagc
    12721 cctgtacact gcctatgaat acttttttgt ttacgggaag gtcaatggta tctaccagca
    12781 actgacccca cttaaaatta cctggctgga gttctctaaa ggtgtatcca tagatatggg
    12841 cctgatgata gaccccatat cggtaatgat gctggtggta gttacccttg tttcgctgat
    12901 ggtacacatc tacagtatcg gctacatgaa aggagaagaa aggttctcca cctactatgc
    12961 ctttcttgga ctatttactt tctcaatgct aggtctggtg ttatctacta acatcttcca
    13021 gatctatatt ttttgggaa
//
LOCUS       DAORLS010000051        12865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2599_length_12865_cov_2.287119, whole genome shotgun sequence.
ACCESSION   DAORLS010000051 DAORLS010000000
VERSION     DAORLS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_12865_cov_2.287119"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..795
                     /locus_tag="PK977_05810"
     CDS             19..795
                     /locus_tag="PK977_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF17660.1"
                     /translation="MEQVLTIKQESKVLEVTENVLVIKSLYKSFGENHVLADFNLELR
                     KGENVAVLGKSGSGKSVLIKCIIGLLVPDTGSINVLGRNIEELSIHEMDELRTKIGFL
                     FQSNALYDSMTVRENLEFPLRRHWIKLSAGERDKKVMEVLTNVGLAHTVDMMPVELSG
                     GMRKRIALARTLILDPEIILYDEPTTGLDPITGKGISTLIKEMGEKYNTSALIISHDM
                     SCIRATADRIVMLIDGRCYAGGTYEELRENKDPKVKQFFE"
     gene            795..1769
                     /locus_tag="PK977_05815"
     CDS             795..1769
                     /locus_tag="PK977_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRF17661.1"
                     /translation="MAKRAINNVKLGFFVLAGLLIMIFSLYMIGKDTNMFGSNYTLKV
                     RFENVHGLTSGNNVRYSGIQVGTVKRVKILSDTLIEVSMLIDTKMKQYIHKNDIVSMS
                     TDGLMGNRILNITPAKDGSPLAENGDLLITKKSVSTDEMLETLDKTNKNIRDISQELK
                     LTVLKINNSSALWKLLTEETLPDNLRASSFSIRKAAANADGMVQDLHTIINDLRAGKG
                     SLGSILTDTAIAYNLNEAVLSIQQVGNHADELVRELDDLTKGLKSDVNSGKGPAHAIL
                     KDSTLVIKLNNSLSNIEKGTEAFNQNMEALKHNFLLRGYFKRLEKRKK"
     gene            complement(1808..2776)
                     /locus_tag="PK977_05820"
     CDS             complement(1808..2776)
                     /locus_tag="PK977_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17662.1"
                     /translation="MSNKNYRLTRTTLTVLLLIAFFANTIAQTTWSENQDTISVQANW
                     KKGDQKTFLITRTKQKKVSGDVRENFQFSFLADMKIVDSTESGYRVEWEYRLTNDFKE
                     KNPLASLAMPVYNDMKMIFTTYTDGSFKDLLNWQEVADAYIEMTELNMGGQKNDTIKK
                     TMDKVKEMFRTKEMTESSLIRELQLYYSAFGGVFTLKGMKSNTTLPNPINAEPLPAYI
                     EQKIKEIKPAEGTFNVEISQSIKPEAMRNLLYGLLEKMDIPRDSIELEMEKMLQSLII
                     TDKGTFTIDQTSGWLLGIEYIREAQFGEIYQKEFILFELVKDKKEQ"
     gene            complement(2780..>3254)
                     /locus_tag="PK977_05825"
     CDS             complement(2780..>3254)
                     /locus_tag="PK977_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRF17663.1"
                     /translation="GSNKTLAAMRRRYKRELYAERVSLIKTLMPHCAIGVDVIVGFPG
                     ETDEDFRETFDFLHSLDVSYLHVFTYSERDNTHALTIKPVVPVSVRNERNKALRNLSY
                     MKMQYFTQQHAGQTRKVLFEGHDKTGMMEGYTDNYIRITTPYREEWVNEIVDWKI"
     assembly_gap    3255..3264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3265..4059)
                     /locus_tag="PK977_05830"
     CDS             complement(<3265..4059)
                     /locus_tag="PK977_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF17664.1"
                     /translation="MAFHTLGCKLNFSETSSLSRMLEQEGFEKKEFDDLADVYVINTC
                     SVTDNADKECRQLVRRIQRKNPESFVVITGCYAQLKPKEISAIPGVDLVLGAAEKFNI
                     AKHIRELSKGDSAKICSCEIEEVSGFHSSWSVNDRTRTFLKVQDGCDYTCSFCTIPMA
                     RGKSRSDSINNVVKNAEALAASGVKEIVLTGVNLGDFGKGPDGAGAAVSISGREENFF
                     ELIKELDKVPGIQRYRISSIEPNLLTNEIIEFVANSNKFMPHFHIPL"
     gene            4233..4667
                     /locus_tag="PK977_05835"
     CDS             4233..4667
                     /locus_tag="PK977_05835"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRF17665.1"
                     /translation="MAIAIIVIKQYKNSNEPAPKPKITNSDKPKDPASNPTSRDRGFD
                     RRTSYLEYSNHATCRMQCRKISKAEVEEIMQLGKINYNKSDLQNARCPRYALEGITKD
                     NQRVRIIFAQCNEKTVVVTVIDLETDYQCNCPGDDDKYKNRN"
     gene            4667..5443
                     /locus_tag="PK977_05840"
     CDS             4667..5443
                     /locus_tag="PK977_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRF17666.1"
                     /translation="MKVLLKPLQWIYSIYAFITFVAIMLLLFPFAVIASFFGRIKGGN
                     MVMRICMIWADIWFLFIFIWHKKIYESPHDKSKSYIFVSNHISYLDAAIIPKAYRQPI
                     RPLGKVEMSKVPIFGFIYRNAIVTVDRSSPSNRANSVRILKSLINKGISVLVFPEGTF
                     NMTTQPLKAFYDGAFRVAIETQTPVKPVLFLDAYRRMPYESLFRMTPGRSRIVYLDEI
                     PVTGFTTADVGLLKDEVFALMERKLVEYDAAWRRDPLNLP"
     gene            5484..>6235
                     /locus_tag="PK977_05845"
     CDS             5484..>6235
                     /locus_tag="PK977_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRF17667.1"
                     /translation="MSKDNFNNEALNNPAGLSSGQAGAGGYAEIIIPAALPKNYTWSV
                     PVHLRELVKPGCRVEVNLGKSKKYAGLVKRLHAERPEFFETKDILNVLDAEPVVFEEQ
                     LKLWEWIASYYMCSEGEVMAAALPAHFKLSSETILVYNEEYGDDFSALDHDEYIVGEA
                     LLLKKELNLSEVQQLLDSSHVYPVINRLINKKVCYVWEALKQTYVPKKETFVLLNPEY
                     DNEDKLSDLLNNWTRAPKQMELLLSYLHLIKT"
     assembly_gap    6236..6380
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6429..>6757
                     /locus_tag="PK977_05850"
     CDS             6429..>6757
                     /locus_tag="PK977_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF17668.1"
                     /translation="MLNKAVCLLHGVTSSGKTNIYIKLIEEYIKLGKQVLYMLPEIAL
                     TSQVIRRLQKHFGGYIGIYHSKFSQNERVEIWNKVKNGELRVILGARSSVFLPFQNLG
                     LVICDEE"
     assembly_gap    6758..6769
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6770..8022
                     /gene="priA"
                     /locus_tag="PK977_05855"
     CDS             <6770..8022
                     /gene="priA"
                     /locus_tag="PK977_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRF17669.1"
                     /translation="SSFKQQEPAPRYNGRDAAIYFASLFKAKTLLGSGTPSIESYYNA
                     VTGKYGLAELLQRYGDLKLPPIEFIDTRMLRQAQHDKPNVSKVMLSTPLIEAVNEVIA
                     RGKQVILFQNRRGYAPYQVCSICGWITQCKYCDVSLTFHKLSNKLVCHYCGTTYPPVH
                     SCAACGSDKFVQRNFGTEKIEEQLQEIFPDAKVARMDIDTVKGKNAHDVLIQQFEQKR
                     IDILVGTQMVVKGLDFDNVDLVGILDADGLLHFADFRVNERAFQLMEQVSGRAGRKEE
                     TGKVLVQTSQPHHPLLKIVQQHDYKAMFTEEVKKRNEFSYPPFTRIIHLTFKHKFKEV
                     AERAAVQYTNALKSKYGNYIVGPAEPVIGRIRNQYLMELLVKLPRDSKTINQCKKDLL
                     EQIAILHQDKSFRSVTVVADVDAV"
     gene            8061..8654
                     /locus_tag="PK977_05860"
     CDS             8061..8654
                     /locus_tag="PK977_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17670.1"
                     /translation="MKKHHFWRLIWVLSPLLFACKGNSSKEAISNLPENVGEKDNGIL
                     SVTDLLELSAYKDLSGVQLYMKKWSENFIHAKKGEFVSFRNVAVTDTAGKNIELTASV
                     LYVATDPMADWRIAHIIHSPELNEAFMKDLKEKGYELSDSSQSYLMKAKSYYYDSEKQ
                     PGKRIVFTRTFMPWLLKGIYVNQVTWPCFVYEVQAKK"
     gene            complement(8656..>9069)
                     /locus_tag="PK977_05865"
     CDS             complement(8656..>9069)
                     /locus_tag="PK977_05865"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17671.1"
                     /translation="GDKDAYRKIDYDITVNAARFCKLNGCETFVFVSTVGANSKSNNF
                     YLKLKGEIEEAVKTVGLTNVYIMQPSLLLGKRKESRFGEKIAQWIMPLLSFVTPAKYK
                     PVKATYVAKAMIRLVKEKPNDLSIATYREIKKMNS"
     assembly_gap    9070..9080
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9081..9303)
                     /locus_tag="PK977_05870"
     CDS             complement(<9081..9303)
                     /locus_tag="PK977_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRF17672.1"
                     /translation="MRATLIGATGLIGSHLLELLLADEYFDSVRILIRRPMEFTHPKL
                     EKKLVDFSDMESFRLALEDSDVVFCSIGTT"
     gene            9326..>9678
                     /locus_tag="PK977_05875"
     CDS             9326..>9678
                     /locus_tag="PK977_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF17673.1"
                     /translation="MQIQENISLRPYNTFGIDTKARYFASFSDTDELEETLNFKLQTS
                     NLILGGGSNILFTKDFDGLVLKNEIKGIAELHEDSEYVYVKAGAGENWHQFVQHCIQR
                     NWAGIENLSLIPGNVG"
     assembly_gap    9679..9751
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9752..10342
                     /locus_tag="PK977_05880"
     CDS             <9752..10342
                     /locus_tag="PK977_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HRF17674.1"
                     /translation="AYHIQERKIVTFTRSDCEFGYRESVFKRKYKDQFVILNVTYQLR
                     KKPRFNTSYGAINQELDRMGVGELSIKSISDAVINIRTSKLPNPKELGNAGSFFKNPA
                     VDTVTFNVLQSKFENIVGYPTADGSVKLAAGWLIEQCGWKGFRKGDAGCHARQALVLV
                     NYGNATGKEIYQLSDEILQSVKEKFGVTMEREVNII"
     gene            complement(10372..>10935)
                     /locus_tag="PK977_05885"
     CDS             complement(10372..>10935)
                     /locus_tag="PK977_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF17675.1"
                     /translation="AERRGIINSEKQTQVWISDLAGIIPSITGKVELVYEGEQEGPFQ
                     VAMNLVDKAIRSQFIQYFPNPDQLKKRRNTGKPSQEEQKDDNPYRPVTNWFDKGNTLN
                     ISFNTSDKDKMLLLYKVDGLHALVKKYFPGSNEEQASLLMEFVLHGLASYSLISKKMF
                     ESKVEFKDLVGSMMNFSDKDFEFDEEA"
     assembly_gap    10936..10959
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10960..11910)
                     /locus_tag="PK977_05890"
     CDS             complement(<10960..11910)
                     /locus_tag="PK977_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRF17676.1"
                     /translation="MNIKNIKTLGELKRSDYQPKSIKEEIRQNLIAKLKKGETTFSGI
                     VGYEDTVIPDVERALLSKHNILFLGLRGQAKTRMARQMTELLDEYIPSIAGSDINDDP
                     LNPISRFAQEEIATHGDETAIHWIHRSERYGEKLATPDVSVADLIGDIDPIKAANLKL
                     SFADDRVIHYGIIPRSNRGIFVINELPDLQARIQVALFNILEEGDIQIRGFKLRLPLD
                     ILFVFTANPEDYTNRGSIVTPLKDRIQSQILTHYPKSLENALEITEQEAEVSDEQKAK
                     VKVSDLIKRLIEQVAFEARNSEFVDKKSGVSARLTISAFEN"
     gene            complement(11991..>12865)
                     /locus_tag="PK977_05895"
     CDS             complement(11991..>12865)
                     /locus_tag="PK977_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF17677.1"
                     /translation="NNEQGQIKITPKTEQGIRKRSLEEIFGKLKKTKQGDHHSFKPGQ
                     GDEVNPETRQFQFGDMLEQIDFTESIRNAQINHGIESFHMKEDDLSIRETDFKAQTST
                     VLMIDISHSMILYGEDRITPAKKVAMALSELIKTKYPKDTLDIVVFGNDAWPIEVKDL
                     PYLQVGPYHTNTVAGLELAMDILRRRKNPNKQIFMITDGKPTCLKIGKRYYKNSFGLD
                     RKVVNRCLNLAAQCKKLKITITTFMIATDPYLQSFVQEFTEMNHGKAFFASLDKLGAF
                     IFKDFESGKRRTVY"
BASE COUNT         3636 a         2447 c         2740 g         3767 t
ORIGIN      
        1 cggacttaac taagggtaat ggaacaagta ttaaccataa aacaagaaag taaggtgctg
       61 gaggtgaccg aaaatgtact ggtgattaaa agcctgtaca aatcttttgg agaaaaccat
      121 gtactggcag actttaacct tgaattgcgc aaaggcgaga atgtggctgt attgggtaaa
      181 tcgggatcgg gtaaatcagt attgataaaa tgcatcattg gcttattggt tccggatacc
      241 gggagtatca atgtgctggg acgaaatatt gaagaactaa gcattcacga gatggatgaa
      301 ttacgaacaa aaatcggatt tctttttcaa agcaatgcat tgtacgattc aatgactgta
      361 cgggaaaatc ttgagtttcc tttaaggcgg cactggataa aactaagtgc cggtgagaga
      421 gataaaaaag taatggaggt gctgacgaat gttggcctgg cccacacggt agatatgatg
      481 cctgtcgaat tgtcgggagg tatgcgtaaa cgaattgcgc tggcaagaac attgatcctt
      541 gatccggaga tcatattgta cgatgagcca accactggtt tggatccaat aacaggaaag
      601 ggcatcagta cactgataaa agaaatggga gagaagtata atacctctgc attgatcatt
      661 tcgcatgaca tgagctgcat acgggcaacg gctgacagga ttgtgatgct gattgatggc
      721 cgatgttatg cggggggaac ctatgaagaa ctgagagaaa acaaagatcc taaagtcaaa
      781 caattttttg aataatggca aagagagcaa tcaacaatgt aaaacttggt ttttttgttc
      841 tggccgggtt gttaatcatg attttttcat tgtatatgat cggaaaggat accaatatgt
      901 ttggcagcaa ttataccctg aaggtaaggt ttgaaaatgt gcatgggctt acatctggaa
      961 ataatgtcag gtattcaggt attcaggttg ggactgtaaa aagagttaag atacttagcg
     1021 atacgcttat tgaggtatcc atgctgattg acactaaaat gaagcagtat atccataaga
     1081 atgatatagt aagtatgagt accgacgggc tgatgggaaa caggattttg aatattactc
     1141 cggccaagga tggctcacca cttgctgaaa acggggattt gctgattacc aagaaaagtg
     1201 taagtactga tgaaatgctg gagacattag ataaaaccaa taaaaacata cgggatattt
     1261 ctcaagagtt gaaattaact gtcctcaaaa taaataatag ctcagcgcta tggaagttat
     1321 taactgaaga aacactgcct gataacctga gggcttcttc tttcagcatc aggaaggcag
     1381 ctgccaatgc tgatggcatg gtgcaggacc tgcatacaat tatcaatgat ttaagggcag
     1441 gtaagggatc cctcggttca atacttactg acacggccat tgcgtataac cttaacgaag
     1501 ctgtactcag cattcagcag gtaggtaatc atgccgatga gttggtcagg gaactggatg
     1561 acttaaccaa ggggcttaaa agcgatgtta acagcggcaa agggcctgca catgctattc
     1621 tcaaagattc aacactggta ataaagctga acaacagtct ttccaatatt gagaagggga
     1681 cagaagcctt taatcaaaat atggaggcgc tgaagcacaa tttcttgtta agaggatatt
     1741 ttaagagact ggagaaaagg aaaaagtgat tatttgaaga gtggggtatt tattcagcca
     1801 aactgaatta ctgttctttt ttatctttta ctaactcaaa cagtataaat tctttttgat
     1861 aaatttctcc aaattgagct tcgcggatgt attcaattcc aagcagccat ccagaggtct
     1921 gatcaattgt aaaggttccc ttgtcggtaa taataaggct ttggagcatt ttctccattt
     1981 ccagttcaat tgaatccctg gggatatcca ttttttccag aagaccatag agcagatttc
     2041 gcatggcttc tggttttatc gactggctga tctcaacatt aaaagttcct tccgctggtt
     2101 taatttcttt tattttttgt tcaatatatg cgggtaatgg ctctgcgttg atgggattgg
     2161 gcaaggttgt atttgacttc attcccttga gggtaaaaac tccgccaaag gcagagtagt
     2221 atagttgcag ttcccttata agtgatgatt ctgtcatctc tttggttctg aacatctctt
     2281 ttaccttgtc cattgttttt ttaatagtat catttttttg cccgcccata ttaagttctg
     2341 tcatttcaat gtatgcatca gcaacttcct gccagttcag caaatccttg aatgagccat
     2401 cagtataggt ggtaaaaatc attttcatgt cattgtagac cggcatagcc agtgatgcca
     2461 gaggattctt ttctttaaaa tcattagtta accgatattc ccattctacc cggtaaccac
     2521 tctctgtaga atctacaatt ttcatgtcag ccaaaaagct gaattgaaag ttttccctga
     2581 cgtcgccaga aacctttttc tgttttgttc tggtaatcag aaaggttttt tgatcacctt
     2641 ttttccagtt tgcctgcaca ctgatagtat cctgattttc tgaccaggtt gtttgtgcaa
     2701 tggtattggc aaaaaatgca ataagtaaca gtacagttaa tgttgttctg gtcaacctgt
     2761 agtttttatt tgacaaagat tagattttcc aatctacaat ttcatttacc cattcttctc
     2821 tataaggtgt ggtgatccgg atataattat ctgtatagcc ttccatcata cctgttttat
     2881 catgtccttc aaataatact ttacgggttt gaccagcatg ctgttgtgtg aagtattgca
     2941 tcttcatata agataagtta cgaagcgctt tatttctttc gtttcttact gatacgggta
     3001 caacaggttt tatagtgagt gcatgggtat tatctctttc tgaataggta aaaacatgga
     3061 gataggaaac atccagtgaa tgtaaaaagt caaatgtttc tctgaaatcc tcatctgttt
     3121 cacccggaaa accaactata acatccacgc caatagcgca gtgtggcatc agtgttttga
     3181 tcagggatac tctttctgca tataattccc ttttataccg gcgccgcatg gctgccaatg
     3241 ttttgttgct gccgnnnnnn nnnncaatgg aatatgaaaa tggggcatga atttattgct
     3301 gttggcaaca aactcaataa tctcattcgt aagaaggttg ggctctatgg aagagatgcg
     3361 gtaacgctga atacctggca ctttatccag ctctttaatc agctcaaaaa agttctcttc
     3421 ccgtccgctg atactaacgg cagcacctgc tccgtcaggg cctttgccaa aatctcccaa
     3481 gttcacaccg gttagtacga tctccttcac accgctggct gccagtgctt cggcattctt
     3541 taccacatta ttaatgctat cacttcggct ttttcctcta gccattggaa ttgtacaaaa
     3601 tgaacaggta tagtcacagc catcctgaac tttcaggaaa gtccttgtcc tgtcattaac
     3661 agaccaggaa gaatgaaacc ctgaaacttc ttcaatttca cagctgcaga ttttggcaga
     3721 gtcacctttt gacagttccc ggatatgttt ggcaatattg aatttttctg cagcacccag
     3781 tacaaggtct acaccgggta ttgctgatat ttcttttggc tttagttgtg catagcagcc
     3841 agtaatgaca acaaaacttt ccgggttttt acgctgaatg cggcgtacca gttgccggca
     3901 ttctttgtca gcattatcag ttacggagca ggtattaata acatacacat cggcaaggtc
     3961 gtcaaactct tttttttcaa aaccctcctg ttccagcatg cgggaaagag aagaagtttc
     4021 ggaaaaattg agcttgcagc cgagggtatg aaacgccact gttcgtgatt ctgccataag
     4081 aggcgcaaag ataggttgat aagttgactg gttgaaatgt tgataagata tgctgtactt
     4141 gttaacttgc tggccattca attttttcta tacatttatt taaatatctg aaagtgaaca
     4201 ggaaatggct tccatatgtt ttgataacct tgatggcaat tgccattatt gttataaagc
     4261 aatataaaaa cagtaatgaa ccggcgccaa aaccaaagat taccaatagc gacaagccta
     4321 aagatccggc cagtaacccg actagccggg acaggggatt tgaccgcaga acatcatatc
     4381 tcgaatacag taaccatgct acctgccgta tgcagtgccg gaagatatca aaggcagagg
     4441 tagaagagat catgcagttg gggaagatca attataataa aagcgacctg caaaatgccc
     4501 gttgcccacg atatgcatta gaaggaataa cgaaagataa tcagcgggta cgtatcatct
     4561 ttgcacaatg caatgaaaaa acggtggtgg taacagtgat tgacctggaa acagattacc
     4621 aatgtaattg cccgggtgat gatgataaat acaaaaaccg caactgatga aggtattgct
     4681 aaaacctttg caatggattt atagcattta tgcctttatc actttcgtgg caatcatgct
     4741 attgcttttt ccttttgccg ttatcgctag tttctttggc cgcataaaag ggggaaacat
     4801 ggtgatgagg atttgtatga tctgggcaga tatctggttt ctttttattt ttatttggca
     4861 taaaaaaatc tatgaatcac ctcatgacaa atcaaaatcc tacatttttg tcagcaatca
     4921 tatttcttat ctcgatgcag ccattattcc aaaggcttat cggcagccca ttcgtcccct
     4981 tggtaaagtt gaaatgagta aagtgccaat atttgggttt atttaccgga atgcgattgt
     5041 aacagttgac agaagcagtc cttcgaacag ggctaatagt gtaagaatat taaagtcact
     5101 catcaataaa gggatttctg tattggtttt tcctgaggga accttcaaca tgacgactca
     5161 gccattaaag gcattctatg acggggcttt tcgtgtggct attgaaacgc agacaccggt
     5221 taagcctgtt ttatttctgg atgcatacag aagaatgccg tatgaaagcc tgttcaggat
     5281 gacgccgggc cgtagccgta ttgtttattt ggatgagata cctgtaaccg ggtttacgac
     5341 tgcagatgtt ggattactca aagacgaagt atttgccctg atggagagaa aacttgtgga
     5401 atatgacgca gcatggagaa gagatcctct taatcttccc taaagggcag actttgggtt
     5461 cctataatta gatttgcctt ttcatgtcaa aagataactt caataatgaa gctttaaaca
     5521 atcctgcagg tctgtcaagc ggacaggcag gagctggagg gtatgcagaa atcattatac
     5581 cggcagccct tcctaaaaat tatacttggt ctgtgcctgt tcatttacgg gaactggtca
     5641 agccaggctg ccgggtagaa gtgaacctgg ggaagagtaa gaaatatgca gggctggtga
     5701 agcggctcca tgcagaaagg ccggagtttt ttgaaacaaa ggatattttg aatgtattgg
     5761 acgctgagcc ggtggttttt gaggagcagt tgaaattgtg ggaatggatc gcttcttact
     5821 atatgtgcag tgaaggggaa gtgatggcag ctgccctgcc agcacatttc aaattatcaa
     5881 gcgaaacaat actggtgtat aatgaagaat atggagatga tttttcagcg ctggatcatg
     5941 atgaatatat agttggagaa gcattgctct taaagaagga acttaatttg tccgaggttc
     6001 agcaattgct tgattcttca catgtttatc ctgtaataaa ccggctgatc aataaaaaag
     6061 tttgctatgt atgggaagca ttgaagcaaa cgtatgttcc caaaaaggaa acctttgtgc
     6121 tgctaaaccc ggagtatgat aatgaagata agctgtctga cctgctcaac aactggacaa
     6181 gggcgcccaa acagatggag cttttgctca gctatctcca tttgataaag acagannnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn gagttgacag aagcaaaata aaaagccttt gatggagtaa
     6421 aaaatcaatt gctcaataaa gcagtttgtc tgctgcatgg cgttacctcc agtggtaaaa
     6481 ccaatattta tattaaactg atcgaagaat atataaaact gggtaagcag gtcttgtata
     6541 tgttgccgga gattgcctta acctcgcagg tgattcgccg tctgcagaaa cattttggtg
     6601 gctacatcgg tatctatcac tccaaatttt cacaaaacga gagggtggaa atatggaaca
     6661 aggttaaaaa tggagaactg cgggtcatac tcggtgcaag gtcgtcggtg ttcctgcctt
     6721 tccaaaacct gggactggta atctgtgatg aagaacannn nnnnnnnnna ttcttctttt
     6781 aaacaacagg agcctgcacc ccgttacaac ggaagagatg cggctattta ctttgcatcg
     6841 ctgtttaaag ctaagacttt gctaggtagt ggtacgcctt ccattgaaag ttattacaat
     6901 gcggttaccg gaaaatatgg cctggcagaa ctgctgcagc gttatggcga tctcaaactg
     6961 ccacctatcg aattcattga tacaagaatg cttcggcagg ctcagcatga caaaccgaat
     7021 gtttccaaag taatgttatc aacgccattg attgaggccg tgaatgaagt tattgcaaga
     7081 ggtaaacagg taatactttt tcaaaaccga aggggttatg cgccctacca ggtttgcagt
     7141 atctgcggct ggataacgca atgcaagtat tgcgacgtat cacttacttt ccataaactc
     7201 agcaacaaac tggtttgcca ctattgcggc actacttacc cgccggtaca tagctgtgca
     7261 gcctgcggca gtgacaagtt tgtacaacgc aatttcggta cggaaaaaat tgaagaacag
     7321 ctgcaggaaa tttttcctga tgccaaagtt gcccggatgg atattgatac ggtaaaaggt
     7381 aaaaatgccc atgatgtgct gatacagcag tttgaacaaa aacgtattga tatattggta
     7441 ggcacacaaa tggtggttaa agggcttgat tttgacaatg ttgatctggt gggcattctg
     7501 gatgcagatg gcttattgca ttttgccgat tttcgggtga atgaaagagc ttttcagctg
     7561 atggaacagg tgagcggccg tgccgggcga aaggaagaaa ccgggaaagt acttgtacaa
     7621 acatcccagc cgcatcatcc gttactgaaa atagtgcagc aacatgatta caaagcaatg
     7681 ttcacagaag aagtgaaaaa acggaacgaa ttttcctatc ctcctttcac ccgaatcatt
     7741 catcttacgt tcaagcataa gtttaaagaa gtagcagaga gagcagcagt gcagtacacc
     7801 aatgcactga agagcaagta tggcaattac atagtcggcc ctgccgaacc ggttattggc
     7861 cgtatccgta accagtattt aatggaactg ttggtgaaac tgccccgtga cagcaaaacg
     7921 attaatcaat gtaagaaaga cttgctggaa caaattgcta tcctgcacca ggataaaagt
     7981 ttccggagtg taacggtagt ggctgatgtg gatgcagtat agaacaccat aaattaataa
     8041 attgatttaa tctcgaaaca atgaaaaaac atcacttctg gagattaata tgggtgctgt
     8101 cccctttact ttttgcctgt aaagggaata gcagtaaaga agcgatcagc aatcttcctg
     8161 aaaatgttgg tgaaaaagat aatgggatac tatccgttac tgatttgtta gagctttcgg
     8221 cgtataagga tctgtcaggg gttcaattgt acatgaaaaa atggagcgaa aactttattc
     8281 atgcaaaaaa aggtgagttt gtatctttta gaaatgttgc ggttactgat actgccggta
     8341 aaaatattga gctaacagct tccgttttat acgttgctac agatccaatg gccgattgga
     8401 gaatagccca tattatacac agccccgaat taaatgaagc atttatgaaa gacctgaagg
     8461 aaaaagggta tgagctttca gactcttccc aaagctattt gatgaaagca aaaagttatt
     8521 attatgattc agaaaagcag ccaggtaaaa ggattgtctt tacacgaaca tttatgccat
     8581 ggcttttaaa gggtatctat gttaatcaag tgacatggcc ttgttttgtg tacgaggtac
     8641 aggctaaaaa atagctcagc tgttcatctt ttttatttct ctataggttg cgatagataa
     8701 atcattgggt ttttccttta ccagccgaat cattgctttt gcaacatagg tggcttttac
     8761 tggtttgtat tttgccggcg ttacaaatga taacagcggc attatccact gcgctatctt
     8821 ttccccgaac cgtgattcct tccttttgcc caataataag gagggctgca tgatatatac
     8881 atttgtcagt ccaacagttt taaccgcttc ttcaatttcg cctttcagtt ttaagtaaaa
     8941 attattgctt ttactgtttg ccccaactgt ggatacaaat acaaaagttt cacaaccatt
     9001 tagtttacag aaccgggcgg catttaccgt tatgtcatag tctatttttc ggtaagcatc
     9061 tttgtcaccn nnnnnnnnnn gagtggtgcc gatactgcaa aataccacgt cgctgtcttc
     9121 cagtgccagc cggaaacttt ccatatcgga gaagtcaacc agtttctttt ccagtttggg
     9181 gtgagtaaat tccatgggcc ggcgaataag tattctgact gaatcaaaat attcatctgc
     9241 aagcagtaat tccagcaagt ggctgccaat aagtcctgta gcaccgatca gtgttgctct
     9301 catcaagaat cccgaagttt gcagtatgca aattcaggaa aatatttcac ttcgcccata
     9361 taatacgttt ggtattgaca caaaagcaag atatttcgca tcgtttagtg acacggatga
     9421 attggaggaa accttaaact ttaaacttca aacttcaaac ctcatccttg gcggcggcag
     9481 taatatttta tttactaagg attttgatgg gctggtgtta aaaaacgaaa taaaaggaat
     9541 tgctgaattg catgaagaca gtgaatatgt ttatgtaaaa gcaggggcag gggagaactg
     9601 gcaccagttt gtgcagcatt gtatccagcg aaattgggca ggtattgaaa acctttcgct
     9661 catccctggc aatgtgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcttatcat attcaggaaa gaaaaatagt
     9781 gactttcacc cggtctgatt gcgagtttgg ctaccgggaa agtgtattca aaagaaaata
     9841 taaagaccag tttgtgatat tgaatgttac ttaccagttg agaaagaagc caagatttaa
     9901 caccagttat ggggctatta accaggaact ggacagaatg ggagtggggg agctttcgat
     9961 caagtccatt tccgatgcag tgatcaatat ccggacttct aaactaccca atccaaaaga
    10021 acttggtaat gctggcagtt tttttaagaa tcccgctgtt gacacggtta catttaatgt
    10081 cctccaatca aaatttgaaa atatagttgg ttatccgact gcagatggca gtgtgaaact
    10141 ggctgccggc tggctgattg agcaatgcgg ctggaaaggt ttccgtaaag gggatgccgg
    10201 atgtcatgcc aggcaggccc tggtgcttgt aaattacggt aatgcaacag gtaaagaaat
    10261 ttaccagcta agtgatgaga ttttgcaaag cgtaaaagaa aaattcggcg tcacaatgga
    10321 aagagaggta aatattatct agtccggttg ttcctatgat tatttaactt ttcatgcttc
    10381 ctcatcaaac tcaaagtctt tatcgctaaa gttcatcatg cttcctacca ggtccttgaa
    10441 ttctacttta ctttcaaaca ttttcttgct gatcagtgaa taggaggcaa ggccatgcaa
    10501 tacaaattcc attaataagg aagcctgttc ttcattagaa cccgggaaat atttcttcac
    10561 caatgcatgc agtccgtcta ctttgtacag cagcagcatt ttgtctttgt ctgatgtgtt
    10621 aaatgagata ttcagtgtat tccctttgtc aaaccagttc gtaacagggc gatatggatt
    10681 atcatccttt tgttcttcct gtgatggttt gcctgtattg cgtctttttt ttagctggtc
    10741 aggattgggg aagtactgga taaactgtga ccgaatagct ttatccacaa ggttcattgc
    10801 cacctggaag ggaccttcct gttctccttc gtaaaccaat tctactttcc cggttataga
    10861 cggaataata ccagccaggt cactgatcca gacctgtgtt tgtttttcgc tattaataat
    10921 gccccttctt tcggcnnnnn nnnnnnnnnn nnnnnnnnna ttttcaaaag cagaaatcgt
    10981 taacctggca cttactccgc ttttcttgtc tacaaactcg ctgtttctgg cttcaaaagc
    11041 tacttgttca ataagccgct tgatcagatc actcactttt actttggcct tttgttcgtc
    11101 acttacttcc gcctcctgtt cagtgatctc cagtgcattt tccaaactct ttggatagtg
    11161 agtgagtatc tggctttgaa tacggtcttt taatggtgtt acaatagagc cacggttggt
    11221 ataatcttcc gggtttgccg tgaacacaaa caggatatcc agcggtaacc gtaacttaaa
    11281 tcctcgtatc tgaatatctc cttcttccag gatgttgaac aaggctacct gtattcttgc
    11341 ctgcaagtct ggtaattcgt tgatgacaaa aatgccgcgg ttgcttcttg ggatgatgcc
    11401 ataatggata acccggtcat cagcaaagct cagtttcagg ttagccgctt taataggatc
    11461 aatgtcaccg atcagatcag caacacttac atccggtgtg gccagttttt caccatatcg
    11521 ttcgctgcgg tgaatccagt gaatagctgt ttcatcacca tgagtagcaa tttcttcctg
    11581 tgcaaaacgg gaaatagggt tcagggggtc atcgttgatg tcactgccgg caatggaagg
    11641 aatgtattcg tccaaaagtt ccgtcatctg ccgggccatt cttgttttgg cctgtccgcg
    11701 gagaccaaga aacagtatgt tatgcttgct cagcaaagct ctttccacat caggtattac
    11761 tgtgtcttca taacctacga tgccagaaaa ggttgtttca cccttcttca gttttgcaat
    11821 gagattttgg cgaatttctt ctttgatgct tttgggctgg tagtcgcttc tctttaattc
    11881 gcccagggtt tttatgtttt ttatattcat ttttctatat atcacccaaa gtttgccaaa
    11941 gggtgaattg tttaagttaa caggattcga cttactcagc ctgacaatga ttagtaaacc
    12001 gttcttcttt ttccactttc aaaatcttta aaaataaagg cacctaactt atcgagtgat
    12061 gcaaagaatg ccttaccatg gttcatttcc gtaaactcct gcacaaaact ttgaaggtag
    12121 gggtcagtag caatcataaa agtggtgata gtaattttta gttttttaca ttgtgctgcc
    12181 aagttcaggc aacggttcac cactttacga tccaatccaa aactgttttt gtaataccgc
    12241 ttcccgattt tcaaacaggt gggcttccca tcagtgatca tgaagatctg cttgtttgga
    12301 ttttttcttc ttcgcaatat gtccattgcc agttcaaggc cagccacggt gttggtatga
    12361 taggggccga cctgcaaata gggaaggtct ttcacttcaa tgggccaggc atcgttccca
    12421 aacacaacta tatccaaagt gtcctttgga tattttgttt ttatcagttc gctcagggcc
    12481 atggccactt ttttggcagg agtgatcctg tcttctccat ataagatcat ggagtgggag
    12541 atatcaatca tcagcacagt ggatgtctgt gctttgaaat cagtttcccg tatactcaga
    12601 tcatcttcct tcatatgaaa actctcaata ccatgattga tctgcgcatt gcggatactt
    12661 tctgtaaaat caatttgctc cagcatatcg ccaaactgga attgccttgt ctcaggattt
    12721 acttcatcac cctggcctgg cttgaaactg tgatgatcac cctgttttgt tttttttagt
    12781 ttaccgaata tttcctccag gcttcttttt ctgatgcctt gttcggtttt tggagtgatc
    12841 tttatctgtc cctgctcatt attat
//
LOCUS       DAORLS010000052        12840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2602_length_12840_cov_2.234337, whole genome shotgun sequence.
ACCESSION   DAORLS010000052 DAORLS010000000
VERSION     DAORLS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12840
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_12840_cov_2.234337"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK977_05900"
     CDS             complement(<1..256)
                     /locus_tag="PK977_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17678.1"
                     /translation="MSYFYGDMDISLKEYFNRFHRLPLDAEDAIDEICSYQKLKKNAL
                     LQPIGYTCRTIYFITNGLARIFYLKDGIDVTESFAFEQQII"
     assembly_gap    497..570
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(777..1568)
                     /locus_tag="PK977_05905"
     CDS             complement(777..1568)
                     /locus_tag="PK977_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17679.1"
                     /translation="MRLQHFISLLFCFFLLSCNSKGQLKSGTFVHREYFTDTTAWGID
                     YAGVMENKIWFRDSLIIVEMKAIYDSTSFNDTGVYRVQDFLFFKYVFFDLKTMKCQDY
                     LTFSDSAKPICHYILPKSSTFFWSFFPLESRDEIRDRYREVPDTVLDNVVYKKLTRTF
                     QERGNSFEFVYYLDKNKPQTILHLNRDLEDLYHEYSVFRYDVIPLRRVSSIKISEMKM
                     LSKELNENEEKVMRAWERNLKTTKMPLITYNESSKTCGADMRYRK"
     gene            complement(1572..2759)
                     /locus_tag="PK977_05910"
     CDS             complement(1572..2759)
                     /locus_tag="PK977_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17680.1"
                     /translation="MQRVNRLSRIFDLTVNMQFSICFSLIVLSLSFVSCQKEKQLIKS
                     SSSVASPDFKGSINLNNIVIKEKVSKWLKQQSELNIESRKKITELAIHNLENADLTYE
                     AISKQRTLAILPIIHGRDFFNRDSVTVIENFIFFIDSLGLIQDGYLSKYFPDDQSITE
                     LPQNGLSDIFNHQAISIDGTFEVTNFDEVKQYEFDFFEGKKQEFRLWESRLALPPGGI
                     GNGEICIDWYLTTTIFNGNGEIIDYFETYLFTNCSSPGPGSNNVPPPPLAAIPSFKDV
                     EMLVVNDVYSNGSKNEVIAFYKLSGWKILGDTRQNVFSSARPVGSMLFQYGVAGISSN
                     GNFMAWTENSNNITFGNYSAAAYMPGPTTATAICSGVKTYPNQGVSRDVVKSATWHAS
                     SIL"
     gene            complement(2889..3722)
                     /locus_tag="PK977_05915"
     CDS             complement(2889..3722)
                     /locus_tag="PK977_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HRF17681.1"
                     /translation="MKKAYWNTYHCQNRIITANGRIYGKYCKNRHCTLCCANRKADII
                     NRYLPVISQWPEPYFVTLTIKAVPHRRLRDVMQNITEEFRQIVETYRKRHQRGKGQKL
                     MGIRSLESNFNPQRRTYNPHFHVIVANKEMADILVKEWFERSNPGWTYRKAQKAERVY
                     DNQRAIIEVVKYGSKIFTEPDVNKKAQAKGKEVMYVKALSNIFAAMKGLRIFDRFGFN
                     LPKAEVKRQDARVVTDYQDWHYDMAKYDWINDKGERLSDYTPGASLLWLLGERVDVQL
                     E"
     gene            complement(3931..4257)
                     /locus_tag="PK977_05920"
     CDS             complement(3931..4257)
                     /locus_tag="PK977_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF17682.1"
                     /translation="MVNPFEAIDARLSNIESLLLDLKHSAPKTEKQNDPDELLTVQGA
                     AEFLSLSVPTVYGLISKGELPVMKRSKRCYFSKQDLLAYLKQGRKKTIRETEQAADDY
                     LIQKRK"
     gene            complement(4338..>5068)
                     /locus_tag="PK977_05925"
     CDS             complement(4338..>5068)
                     /locus_tag="PK977_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17683.1"
                     /translation="IGLAQLKNSLRLYGLELGLFIYYYEFDHSNIHSAFHASPWKEYE
                     DIKTMNELSFKIDYLKTDVKDLDDYFSFHYRSFKRKHEKSPDWETKRNNWFEETKKNA
                     LFTKDFRHKDAFLNWLGKMENDPVISKVNPGQKKSKVKEVFSQRQIAIACFFLGIQLT
                     KENGKEVLEKYSDTTSVEKLLSKRISKASELTRLSEFKTSDTKHLKDLEAAKRLISGQ
                     KNRKALKDISSVIATFHQNYKAKH"
     assembly_gap    5069..5165
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5347..>7864)
                     /locus_tag="PK977_05930"
     CDS             complement(5347..>7864)
                     /locus_tag="PK977_05930"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRF17684.1"
                     /translation="CHESINKEITLDDVREMIIQHILTEDVFNTIFDETQFHRENNVA
                     RELEKVINSFFTGQTRRLALGTIQHYYQAINAAAAGIADHHEKQKFLKVIYETFYKSY
                     NPKAADRLGVVYTPNEIVQFMIKGTDYLLHKHFNKFLEDKGVDILDPATGTGTFVCDM
                     IDYMGKDKLEYKYKHELHANEVAILPYYISNLNIEFTYTQKMGKYAEFTNLCFVDTLD
                     NIGGLNEKVTGIHSKYKVMGDLFSITKVNSQRIVDQNRKKISVVIGNPPYNANQANEN
                     DNNKNREYPTIDQRIKDTFIKNSTAQKTKVYDMYARFYRWAFDRLDENGIIAFITNRS
                     FIDSRTFDGFRKCVQDEFDYAYIIDTHSDVRANPKIAGTTHNVFGIQAGVAIMFLVKK
                     YSKEKKKPCTIYYVDMPDDWRKEQKLQWFTINPLPNIHFQHINPDKNNNWINIAVNDF
                     ESLLPLIDKSVKSGKSKKALFQLYSNGIVTARDEWTIDFSKIDLIKKVTYFCDFYNKE
                     SSRWHESNKSIQLNDFLDRKIKFGSEIEEYIKKGVQLNFNSDNIVNIEYRPFVKALCY
                     FDRIITHRIYQNELIFGLRNTHENIAICFSGGSSSKPFTALVSKSIFSFDFIEKTQCI
                     PFFHYNKGNSQVINITDWGLNQFTKQYGKKGITKESIFHYVYAVLHNPAYRKKYELNL
                     KREFPRIPFYDDFQQWAAWGKALMDLHINYETVQPYDLGRVEYQFTVDTPEGLQKLQK
                     AKLKADKAAGTIALDGFTSLTGIPAEAWEYKLGNRSALEWILDQYKESKPKDPTIAEK
                     FNTYKFADYKDQVIDLLQRVCTVSVETMKIIKEMENVVTH"
     assembly_gap    7865..7885
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7886..>8250)
                     /locus_tag="PK977_05935"
     CDS             complement(<7886..>8250)
                     /locus_tag="PK977_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17685.1"
                     /translation="RQDWGYWESKDESDDIDEEIRKKFAKGYPKENIFFEDSQTAVLI
                     QNGLEVQRVSMKDAGALHSIISRFISFERPEVQQFRKAIELFKQDIPKVTDTIRGIII
                     AQEHTNPEFTKAAGEFLAL"
     assembly_gap    8251..8268
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8269..8466)
                     /locus_tag="PK977_05940"
     CDS             complement(<8269..8466)
                     /locus_tag="PK977_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17686.1"
                     /translation="MSEFSIRQYQNEVEKIIHFGGTRKETAIRNAFYNLLNEYAKPKG
                     LMIVPEVTIRAANGKNVTPDGT"
     gene            complement(8647..9054)
                     /locus_tag="PK977_05945"
     CDS             complement(8647..9054)
                     /locus_tag="PK977_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HRF17687.1"
                     /translation="MQIIQSIQNRIYEIRGERVMLDRDLAALYEVETKRLKEAVNRNI
                     KRFPEDFMFQLTKEEWEALRFQIETLEKQDNPLRTQIATLKKGRGQHSKYLPYAFTEQ
                     GVAMLSGILNSDRAIQMNIAIMRAFVEIRRVLF"
     gene            complement(9064..10242)
                     /locus_tag="PK977_05950"
     CDS             complement(9064..10242)
                     /locus_tag="PK977_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRF17688.1"
                     /translation="MAIKVKLRQKPISGNRLSLYLDFYPPIIHPETGEPTRREFLGQY
                     LFSETIKDEKGRIKKISISPLDKKHNKETLQIAEQIRQKRENHLNKPEIYTGQEREQL
                     RIKEIGERNFVEYFKQLADKRKASNHDNWISVYNYLLAFTKGFLPFARLNETFCNDFR
                     EFLLSTQSLKRTQAKLSTNSAFSYFNKFKASLKQAFKDGLLQTDLNSRVEPIKPDETQ
                     RNFLTLEELNSLVRTDCKEPLLKMAALFSAMTGLRFSDIQKLVWGEIEFINGNGYVIR
                     FRQQKTKGQETLPVSDQAIELLGQRKSPESKVFEGLNYSAYGNKHLAHWIGAAGITKD
                     ITFHCFRHTFATLQLSQGTDIYTVSKMLGHRDLKTTQIYTKIIDENKRAAANKIKLEL
                     "
     gene            complement(10260..10613)
                     /locus_tag="PK977_05955"
     CDS             complement(10260..10613)
                     /locus_tag="PK977_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF17689.1"
                     /translation="MSSTLRIQRICQHCENEFTARTTVTKYCGDYCAKMAYKVRQRNA
                     RIDVSNRETIAILNKPLEAIKAKEFLTVKEVAALLNCSVRSVYQHINNNKIKAVNLSQ
                     RLTRIKRTDIDKLFA"
     gene            complement(10864..12243)
                     /gene="mnmE"
                     /locus_tag="PK977_05960"
     CDS             complement(10864..12243)
                     /gene="mnmE"
                     /locus_tag="PK977_05960"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRF17690.1"
                     /translation="MQGKLSGWDDTIVALATAHGVGAIAVIRVSGPDAIGICNRLFPS
                     KSLAAQPSHTLHVGFLEHSGRVLDEVVLSLFKGPRSYTGEDVVEISCHGSPYIQEQII
                     SAITAEGARLAKPGEFTQRAFLSGKMDLAQAESVADLIASNTEASRRTALHNMRGGFS
                     HVLQGLRERMLQFSALIELELDFSQEDVEFADRSRFFQLIEEAQHTVGGLIESFRLGN
                     VIRNGVSVAITGKPNAGKSTLLNSLLNENRAIVSDIAGTTRDTIEELINIEGILFRLI
                     DTAGLRETEDVIETIGVEKSMKKMKQADLVLYLFDVQEETPEELEAAQWIVRGQNPQY
                     LLVGNKADTIGLEAAQQKFSGDDVWYISAQTGFHVEQLKRRLVEKVVQGQLQSENTIV
                     TNARHYSALQQVQQSIYDIRKGLDDQLPGDLLALDIRRCLHYLGEITGEVSNEDRLDY
                     IFSKFCIGK"
     gene            complement(12328..>12840)
                     /locus_tag="PK977_05965"
     CDS             complement(12328..>12840)
                     /locus_tag="PK977_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF17691.1"
                     /translation="TPPKIVKDEEVKPDEKIEEIKEDQVISTKTVESENKEQIVQAPV
                     EDKGTQVVEVPKVSDDENKIFTKVEVEASFPGGESAWRRYLQNNLNPNTPVDNGAPEG
                     TYQVVVRFIVGRDGSISDVQAETSHGYGMEQEAMKIIKKGPKWTPAIQNGRNVNAYRR
                     QPITFVVQEQ"
BASE COUNT         3391 a         2863 c         2265 g         4111 t
ORIGIN      
        1 caatgatttg ttgttcaaag gcaaagctct ccgtcacgtc tattccatct tttaaataaa
       61 aaatcctggc aaggccattt gtaataaaat aaatggtcct gcaggtataa ccaattggtt
      121 gcagcaatgc atttttcttg agcttctggt aactgcatat ctcatcgata gcatcttcag
      181 cgtccaatgg cagtcggtga aaccggttaa aatattcttt cagggaaata tccatgtcac
      241 cataaaagta tgacacgaac aggaaagttc ataagggcct tggtttcctg gaatacattt
      301 ttgaatggcc agctgcatca aataatttct gttacgtttg ttaaggcttc aatgaaagtg
      361 catacatttt ttttgcttat tctttagcac ggttactttc cgatacagaa actaacttta
      421 gctgatattc aaatagttag aaaaataagg tacaaatagg gtacaaatat gcggtaatac
      481 tcaactttct tagctannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ataataacgc tgactttctt ttatctcaaa
      601 atcaaaaaat tcggtttcct tgccatccac cgggaaccca tcggtctggg cgtacggatt
      661 gggctgcctg gtaaaaccct gatctttatt tttggaattg gccattctta aaattactaa
      721 atgtcccttt cattttgcat ccgtgccatt ccctttgcac tacaaatggg gttgtattat
      781 ttcctatatc gcatgtcggc tccgcaagtc ttagaagact cattgtaggt aattaatggc
      841 atcttggtcg tcttcaagtt tctttcccag gctctcataa ctttttcttc attctcgtta
      901 agttcctttg agagcatttt catctctgaa atttttatgg aacttacacg cctcaagggt
      961 ataacatcgt acctaaagac agaatactcg tgatacaaat cttccagatc cctgtttaga
     1021 tgcagaattg tttgcggctt attcttatcc aaatagtaca caaattcaaa tgaattaccc
     1081 cgttcctgaa aagttcttgt aagttttttg taaacaacat tatccaaaac cgtatcgggg
     1141 acttcccgat acctatctct tatttcatcc cggctctcta atggaaaaaa agaccaaaaa
     1201 aatgtcgaac tcttaggtaa aatataatgg caaattggtt tagcagaatc actaaaggtc
     1261 agataatcct ggcacttcat ggtttttaaa tcaaaaaaaa catacttgaa aaataaaaaa
     1321 tcttgaactc tataaacacc ggtatcatta aaactggttg aatcatatat tgccttcatt
     1381 tcaacaataa taagactgtc acgaaaccaa attttatttt ccatgacacc agcataatca
     1441 ataccccatg ctgttgtatc tgtgaagtat tccctatgaa cgaaagtacc tgactttagc
     1501 tgtcctttag aattacatga taacagaaaa aaacaaaata acaacgatat aaaatgctga
     1561 agtctcataa atcacaatat tgaagacgca tgccaggtag cagacttaac cacatctctt
     1621 gagacacctt gatttggata tgtcttaact cctgaacaga ttgcagttgc cgttgttggg
     1681 ccaggcatat atgcggctgc actataatta ccaaatgtaa tgttgttcga attttccgtc
     1741 catgccataa agttaccatt ggaactaatc cctgcaacgc cgtattgaaa aagcatagat
     1801 cccactggcc ttgctgagga aaatacattc tgtctcgtat ccccaaggat tttccagccg
     1861 gataacttgt aaaaagctat gacttcattc ttagaaccat tactataaac atcatttacg
     1921 acaagcatct ctacatcttt aaaagatgga attgccgcca aaggaggtgg aggcacatta
     1981 ttgcttcctg gaccaggtga cgaacagttt gtaaataagt aagtctcaaa ataatcaata
     2041 atttcaccat tgccgttaaa aatagtagtt gttagatacc aatcaatgca aatttcacca
     2101 ttaccaatcc cgccaggtgg caatgcaaga cgagattccc ataacctaaa ttcctgtttc
     2161 tttccttcaa aaaaatcaaa ttcgtattgt ttaacttcat caaaattagt aacctcaaaa
     2221 gtgccgtcta tagaaattgc ctgatgattg aaaatatctg ataggccatt ttgtggaagc
     2281 tctgtaattg attggtcatc cggaaaatac tttgacaaat atccatcttg tattaaaccc
     2341 aaagaatcta taaaaaatat aaaattttca atcactgtca cgctatctcg attaaaaaaa
     2401 tccctcccat gaataatcgg aaggattgcc aaagtcctct gctttgaaat tgcttcataa
     2461 gtcaaatcgg cattttctaa gttatgaatt gcaagttctg ttatcttctt tcttgattcg
     2521 atatttaatt cagattgttg ttttagccat tttgatactt tctcttttat gacaatatta
     2581 ttaaggttta tggatccctt gaaatcagga gatgctactg aacttgatga ttttatcaat
     2641 tgtttttctt tttggcaact aacaaaactt aatgataaaa ctatcaagct aaagcaaatc
     2701 gaaaactgca tattaacagt taaatcaaaa attctcgata atctatttac gcgttgcata
     2761 aaatttaatt taagataaaa aaaggattat aaataaattt atcgtcaatt ttcttgcata
     2821 cgctgggtga aaaaagctaa tctttagggt gtttttcccc ttgcttaatc aataatatcc
     2881 cgtaaatatt attctaactg cacatccacc cgctcaccta acaaccacaa caaactggcc
     2941 ccaggggtat aatcactaag ccgctcccct ttgtcattaa tccagtcata ttttgccata
     3001 tcataatgcc aatcctggta atcggtcact accctggcat cctgcctttt tacctcagct
     3061 ttgggaagat taaacccgaa acggtcaaat atcctcaggc ctttcatggc ggcgaaaata
     3121 ttgctcaggg ctttcacata cataacctct ttaccctttg cctgggcctt tttgtttacg
     3181 tccggttcgg tgaaaatctt actcccgtat ttcactactt caattatggc ccgttgatta
     3241 tcataaaccc tttcagcttt ctgggccttt cggtaagtcc atccggggtt actcctttca
     3301 aaccactcct ttaccagaat atcagccatt tctttattgg ccactattac atgaaagtgc
     3361 gggttatatg tcctcctttg cggattgaaa ttgctctcca gggacctgat gcccattagc
     3421 ttttggcctt tgccccgctg atgcctttta cggtatgttt ccacgatctg cctgaattcc
     3481 tcggtaatat tttgcattac atcacgtaac cgcctgtgag ggacggcctt aatagtcaac
     3541 gttacaaaat aaggttcggg ccattggctg ataacgggca aataacggtt tataatatcc
     3601 gctttccgat tggcacaaca aagggtacaa tgtcggttct tacaatactt accgtaaatc
     3661 cggccattgg cggttatgat gcggttctgg caatggtatg tattccaata tgcctttttc
     3721 atttccggag tgccgtactt catacaggaa tcaaccatgc ttaacatgag gctctgactg
     3781 atcaatttcc tttttgatct gccctgtaat gctttagcat tggccaggtc gctaccattc
     3841 ccctggatta tggccatttt atctgtcccg ctttgtgcta atgtattgaa tgaccccagg
     3901 catgaggggc catgaggtgt tggcttcatg ttactttctc ttttggatta agtaatcatc
     3961 ggccgcctgt tccgtttccc ggatagtttt tttccggcct tgctttaggt aggccagcaa
     4021 atcttgctta gaaaaatagc agcgtttgga ccgcttcatt acggggagtt cacctttgga
     4081 tattaggccg taaacggtgg gaacggataa gctgagaaat tcagcggcac cctgaacggt
     4141 taggagttcg tccgggtcgt tttgcttttc cgtcttaggg gctgagtgct taaggtccag
     4201 taacaggctt tcaatgttag acagcctggc atcaatagct tcaaaagggt ttaccatttt
     4261 atagacattt agaccctaaa gggaaaaata tctacgaaat gaaaccgctt gcaagtggta
     4321 tgcaagtggt aaaaatctta atgttttgct ttgtaatttt gatgaaatgt agcaatgacg
     4381 gaacttatat cctttaatgc ctttctattt ttttgaccac ttattaaccg cttggcggcc
     4441 tccaggtcct ttaaatgctt tgtatcactt gtcttaaatt cagacaatcg agttaattca
     4501 cttgccttag aaattctttt agataacagc ttttctacac tggttgtatc actatatttt
     4561 tctaaaactt cctttccatt ctcctttgtt aactgaatac ccaaaaagaa acaggcaatg
     4621 gctatttgac gctggctgaa aacttctttt accttgcttt tcttttgccc cggatttact
     4681 tttgaaatca ccggatcatt ctccattttt ccgagccaat tcagaaaagc atctttatgc
     4741 ctgaaatctt tggtaaacag tgcatttttc ttggtttctt caaaccaatt atttcttttg
     4801 gtttcccaat ctggactttt ttcgtgtttg cgcttaaagg aacgataatg aaaactgaaa
     4861 taatcgtcta aatcttttac atccgtcttt aagtaatcaa tcttaaaaga aagctcattc
     4921 attgtcttta tgtcctcata ttctttccag ggagaggcat ggaaagctga atgtatattg
     4981 gagtggtcaa attcataata ataaataaaa agccctaatt ctaatccata tagcctcaga
     5041 ctgtttttta attgtgctaa tccaattcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnaattt cactctcaat ttttttacta taatactccc aactgtcatt cagttttcgc
     5221 caaaataccg agcctgaata tccactctcc ttttcatgtt ttttccaaaa ttcaatccgt
     5281 gaataatcag gctcaaccaa tccgaaaagc aatatttcat taattgtcaa atccatcctc
     5341 aggcatttaa tgagttacaa cattttccat ctccttaata atcttcatag tttccacact
     5401 aaccgtacat acccgctgta ataaatctat tacctggtcc ttataatcag cgaatttgta
     5461 agtattgaat ttctcagcta tggtcgggtc ctttggttta ctttctttgt attggtccaa
     5521 tatccactcc agggcggaac ggttgcccaa tttatattcc catgcctcag cgggtattcc
     5581 ggttaaggag gtgaagccgt ctaatgctat ggttccggcg gctttgtctg cctttaattt
     5641 tgccttttgc agcttttgca atccctcagg cgtatccacg gtaaattgat attctaccct
     5701 gcccaaatca taaggctgca ccgtttcata gttaatatgc aaatccatta aggctttacc
     5761 ccaggctgcc cactgctgga aatcatcata aaaaggtatt cggggaaatt cccgctttag
     5821 gtttagttca tattttttcc ggtaagcagg gttatgcaat acagcatata cataatggaa
     5881 tatactctcc tttgttatac cttttttgcc gtattgctta gtgaattggt taaggcccca
     5941 gtcggtaata tttattactt gactattccc tttattataa tgaaaaaatg ggatacattg
     6001 agttttttct ataaagtcaa aactaaaaat acttttgctt accaatgctg taaacggctt
     6061 tgatgaagat cccccagaaa agcaaatagc tatgttttca tgtgtattcc ttaaaccaaa
     6121 gattaattca ttttgatata tcctatgtgt aattattcta tcaaaataac ataatgcttt
     6181 tacaaaaggc ctgtattcaa tatttacaat attatcggaa ttaaaattta attgtactcc
     6241 tttttttata tactcttcta tttctgatcc aaattttatt ttgcgatcca aaaaatcatt
     6301 caattgtatt gatttatttg attcatgcca ccttgaactc tccttattat aaaaatcaca
     6361 gaaatatgtt acttttttta ttaggtcaat ctttgaaaaa tcaatcgtcc attcatctcg
     6421 tgctgtaact attccattag aataaagctg aaataaagct tttttgcttt tcccagactt
     6481 tactgactta tcaatcaaag gcagtaatga ttcaaaatca ttaactgcaa tatttatcca
     6541 attattattc ttatccgggt taatgtgctg aaaatgaata ttgggaagag gattgattgt
     6601 aaaccattgc agtttttgtt ctttgcgcca atcatccggc atatccacat aataaatggt
     6661 gcagggcttt ttcttttctt tactgtattt ctttaccaga aacataatgg ccacccctgc
     6721 ctgaattcca aatacattat gggtggttcc ggcaattttt gggttcgccc ttacatcact
     6781 gtgcgtatct atgatataag catagtcaaa ttcatcctgc acacattttc taaagccgtc
     6841 aaaggtccgg ctgtcaataa aactcctatt cgtaataaag gcaataatgc cattttcatc
     6901 caatcggtca aaagcccagc ggtaaaaacg ggcatacata tcatacacct tagttttctg
     6961 ggcggtgctg ttctttatga atgtgtcctt tattctctgg tcaatggtcg ggtattccct
     7021 gtttttatta ttgtcgtttt cattggcctg gtttgcatta taaggcgggt taccaatcac
     7081 cacgcttatt ttttttctgt tttggtccac aatcctttgt gaattcactt tggtaatgga
     7141 aaataaatct cccatcactt tgtatttgct atgaatcccg gtaacttttt cattcagccc
     7201 tccgatattg tccagggtat ccacaaaaca caggtttgta aactcggcat atttgcccat
     7261 cttttgggta taggtgaatt caatattcag gttactgata taatagggca gaatggccac
     7321 ttcattggcg tgtaactcat gtttgtattt atactccagc ttatcctttc ccatgtaatc
     7381 aatcatatca caaacaaagg ttccggttcc tgtggccggg tcgagtatat ccacgccttt
     7441 atcctccaga aacttattaa aatgcttatg taacagataa tcggtcccct taatcatgaa
     7501 ttgtacaatc tcattagggg tataaaccac acccaggcgg tccgccgctt tggggttgta
     7561 actcttataa aaggtttcat aaatgacctt taggaatttc tgcttttcat gatgatctgc
     7621 aattccggcg gcggcggcat taatggcctg gtaataatgc tgaatggttc ccagggccag
     7681 gcggcgggtt tgcccggtaa agaaagaatt aataaccttt tccaattccc ttgccacatt
     7741 attctcccgg tgaaactggg tttcatcaaa tatggtattg aatacgtcct cagtcaggat
     7801 atgctgaatg atcatttccc gtacatcatc cagggtaatt tctttattaa tgctctcatg
     7861 acaannnnnn nnnnnnnnnn nnnnnaagtg ccagaaattc cccagccgct ttggtaaatt
     7921 ccgggtttgt gtgttcctgt gcaatgatga ttcccctgat agtgtccgta acttttggaa
     7981 tatcctgctt aaacaattca atagcctttc tgaattgttg cacttctggc cgctcaaagc
     8041 tgataaaacg ggaaatgatg ctatgcaaag ccccggcatc tttcatagaa acccgctgca
     8101 cctccaggcc attctgaatt aataccgccg tttgactgtc ctcaaaaaag atattttctt
     8161 tcgggtatcc ctttgcaaat tttttcctga tctcttcatc aatatcatca ctttcatcct
     8221 tactctccca ataaccccaa tcctgcctga nnnnnnnnnn nnnnnnnngg ttccgtccgg
     8281 ggttacattt ttgccattcg ctgcccgaat ggtaacctca ggtacaatca ttaacccttt
     8341 aggctttgcg tactcattta agaggttata aaaggcgttc ctgatggccg tttcttttct
     8401 cgtgcctcca aaatgaatga tcttttccac ctcattttga tactgcctga ttgaaaattc
     8461 gctcatgtat ttaattcgtt tttgggttta tttcttaaat cctatccttt cccggtcctc
     8521 ccatttacgc tgggcggcct tctcatccaa taaattttcc attgcatcat aaatctggtt
     8581 tagctgggca tcatgctccc ctattctttc cttaatctgc cttaattgag cctttatatc
     8641 actttcctaa aataacaccc gcctgatctc cacaaaggcc cgcatgatgg ctatattcat
     8701 ctgtatggcc cggtcgctgt tcagaatacc ggacagcatg gccacgccct gttcggtaaa
     8761 agcataaggc aggtatttac tatgttgccc tctgcctttc tttaaggtcg caatttgcgt
     8821 ccttaaagga ttatcttgtt tttctaaggt ttcaatttga aacctcaaag cctcccattc
     8881 ctcctttgtt agctgaaaca taaagtcctc aggaaaccgc tttatattac ggtttaccgc
     8941 ctcttttaag cgttttgttt ctacttcata cagggccgct aaatcacggt ccagcatgac
     9001 ccgctcccct ctgatctcat atatccgatt ctgaatactt tgaatgatct gcataggatt
     9061 aatttataat tccagtttga ttttattagc ggctgcccgc ttattttcat caataatttt
     9121 tgtgtaaatc tgtgtggttt tcaggtcccg gtggcccagc attttggaaa cggtataaat
     9181 atccgtaccc tggctgagtt gtaaggtagc aaacgtatgc ctaaagcaat ggaaagttat
     9241 atcttttgtt attccggcgg ctccaatcca atgggccaaa tgtttgtttc cataagccga
     9301 ataatttagc ccttcaaaaa ctttgctttc cggagatttt cgctgcccta ataattcaat
     9361 ggcctgatcg gacacaggga gggtttcctg gccttttgtt ttttgttgac ggaaacggat
     9421 aacataaccg ttcccattaa taaattcaat ctctccccaa actaattttt gaatatccga
     9481 aaaccttaat ccagtcatgg ctgaaaacag ggcggccatt ttcaacaatg gctccttaca
     9541 atcagttctt accaggctat tgagttcctc caatgtcagg aaattacgct gcgtttcatc
     9601 cggcttgatc ggctctaccc ggctattcag gtccgtttgt aaaaggccgt ccttaaaggc
     9661 ttgtttcagg ctggccttga atttattgaa atacgaaaag gcagaattgg tggaaagttt
     9721 agcctgggtt cttttgaggc tttgggtgga taatagaaat tcccggaaat cattacagaa
     9781 tgtttcattc aatctggcaa agggcaaaaa accttttgta aaggccaata ggtagttata
     9841 cacggaaatc cagttatcat gattggaggc tttgcgttta tcggctaact gcttaaagta
     9901 ttccacaaaa ttccgttccc cgatctcctt tattcttaat tgctcccttt cctgcccggt
     9961 atatatttcg ggcttattca ggtggttctc tcttttttgc ctgatctgtt ccgctatctg
    10021 gagggtttct ttattgtgct ttttatccag tgggcttatt gaaatcttct taatcctgcc
    10081 cttttcatct tttatggttt cggaaaacag gtactggcca aggaattccc ggcgggtagg
    10141 ttccccggtt tccgggtgga tgatgggcgg gtaaaaatca aggtaaaggc ttaaacggtt
    10201 gccggatatg ggtttctgcc tgagtttcac ttttatagcc attgtcagat aattaatgtt
    10261 taggcgaata gtttatctat gtcggtccgc tttattcgtg tcaacctttg ggaaaggttc
    10321 acagctttaa ttttattatt gttaatgtgc tggtaaacag atcggaccga acaattcaat
    10381 aaagcggcca cctccttaac ggtcagaaat tcttttgctt taattgcctc taaaggttta
    10441 ttcaatatgg caatagtttc ccggttgctg acatcaatcc tggcatttct ttgccggacc
    10501 ttataagcca ttttagcgca atagtcaccg caatattttg ttacggtggt acgggccgta
    10561 aactcatttt cacaatgctg acaaattcgc tgaattctca gggttgaact catttccttt
    10621 tatgtcgtta aggttgatta ataaaatatt agtgtcgtta agtgcattta agtgcataaa
    10681 aaaacggctg ttttgccgaa attttgtacc tcggccccta aggtacaaat aaggtacaaa
    10741 tatttactaa aaatagtggg aataacacaa aataaaccga taaggataaa aatgacaaaa
    10801 ggccctatta ttgggccttt tgtacggtaa ttcgtgggtt ttttgcttat ttatttagca
    10861 tggttacttt ccgatacaaa acttactaaa tatataatcc aacctgtctt cattgctcac
    10921 ttcaccggtg atctcgccga ggtagtgaag gcaacggcgt atgtctaatg ccaggaggtc
    10981 gccgggcaat tgatcatcca acccttttcg gatatcataa atagattgct gcacttgttg
    11041 taaagcgctg taatgccgcg cattcgtgac tatcgtattc tccgattgca actgcccttg
    11101 taccactttc tccaccagcc tgcgcttcaa ctgttctaca tgaaatccgg tttgcgcgga
    11161 tatataccat acatcgtccc cgctgaattt ttgctgcgcc gcttccaaac caatcgtatc
    11221 cgccttattg cctaccagta aatactgcgg attttgccca cgtactatcc attgcgccgc
    11281 ttccagttct tccggtgttt cttcctgcac gtcaaaaaga tacaatacca ggtctgcctg
    11341 cttcattttc ttcatgctct tctccacacc aatcgtttca attacatcct ccgtctcgcg
    11401 caaacctgca gtatctatca acctgaataa aatgccttca atattgatca gctcttcgat
    11461 cgtatcccgc gtcgttccag cgatatcact cacaatcgcc cggttctcat tcaataaact
    11521 gttcagcagg gtcgatttgc ccgcgttcgg ttttccggtg atggccacac tcacgccatt
    11581 cctgatcaca ttccccaacc ggaacgattc tatcaagccg ccaaccgtgt gctgcgcttc
    11641 ctcaatcaat tgaaaaaaac ggctccggtc ggcaaactcc acatcctcct ggctaaagtc
    11701 cagctcaagc tcaatcaaag ccgaaaactg cagcatccgc tcacgtaatc cctgcaaaac
    11761 atgcgagaag cctccccgca tattatgcaa tgcggtccgc ctcgacgctt ccgtgtttga
    11821 agctatcagg tctgccaccg attccgcctg ggccaggtcc attttacccg ataaaaaagc
    11881 ccgctgcgta aattcacccg gcttcgccaa acgggcgcct tccgctgtaa tggcgctgat
    11941 aatctgttcc tggatatatg gcgaaccatg acaggatatc tccaccacat cctcccccgt
    12001 ataactgcgc gggcccttaa acaacgacaa aaccacttca tccaaaacac ggccactgtg
    12061 ttcaagaaag cctacatgca gggtatggct tggctgtgcc gctaagcttt ttgaaggaaa
    12121 gaggcggttg caaataccaa tggcatcagg cccgctcacc ctgatgaccg caatagcgcc
    12181 aacgccatgc gccgtagcca gggccacaat cgtatcgtcc catccggata atttgccttg
    12241 catgaggtaa agttaaatca ttcatccagc agatgcataa aaaaagcctc ccggttgggg
    12301 aggctaaatt tcgtttaacc gttgtcgtta ttgttcctgt accacgaagg taatcggctg
    12361 acggcggtag gcatttacat tacggccatt ctggatcgcc ggggtccatt tcgggccctt
    12421 cttgatgatc ttcatggctt cctgctccat accataaccg tggctggttt cggcctgcac
    12481 atcactgatg ctgccatcgc ggcccacaat gaaacgcaca accacctggt atgtaccttc
    12541 gggagcgccg ttatctaccg gggtatttgg gttcaggttg ttttgcaggt aacgcctcca
    12601 ggcagattcc ccaccaggga aagatgcttc cacttccacc ttggtaaaga tcttattttc
    12661 atcgtccgac actttgggaa cttccacaac ctgtgtaccc ttatcttcca caggagcctg
    12721 aacaatttgt tccttgttct cgctctccac ggtcttggta ctgatcacct ggtcttcctt
    12781 gatctcttcg atcttttcat caggcttcac ttcctcatcc tttacgattt tcggaggggt
//
LOCUS       DAORLS010000053        12824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2610_length_12824_cov_2.186154, whole genome shotgun sequence.
ACCESSION   DAORLS010000053 DAORLS010000000
VERSION     DAORLS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_12824_cov_2.186154"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PK977_05970"
     CDS             complement(<1..720)
                     /locus_tag="PK977_05970"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRF17692.1"
                     /translation="MPHKFFISHYSGDKAIAELFSGALRRITLEQISPWFSSDSTGGS
                     GLKPGDIWFNQILSKITQSKAVVSLLTPNSVNRPWVYFESGIGQALENCEVIPVCIGI
                     NRDSILPPLGLYQCYQLNDYRSVVEFFSKLLALFEIKFDEEMSKVVIEKLVTEISKIT
                     FEEQEKTEENTETVEKLIENFKNHIDKRFLEVLEKPTYAISGDNLKVHVEDIKTQAFT
                     EDVTYAVQFDIDFPKLKNKNLF"
     gene            complement(1374..1550)
                     /locus_tag="PK977_05975"
     CDS             complement(1374..1550)
                     /locus_tag="PK977_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17693.1"
                     /translation="MVNLAIVGCLLGNDLYKKKNYKPFLVVFIVFLVGAVMWRMRDSD
                     EWQSLAKVYATLFY"
     gene            complement(1579..>1997)
                     /locus_tag="PK977_05980"
     CDS             complement(1579..>1997)
                     /locus_tag="PK977_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17694.1"
                     /translation="KYYISQFPNFINKTTIIHIHGALWMTWLALLVVQPFLIYTGRPQ
                     LHRAIGKVAYVHGPLLILFLFLAGRESYWRIMENANEQAALKFIVLDSRGLFSFAIFW
                     TLAMLFRKHPASHMRYMIATGLLAIGPGIGRQQFQP"
     assembly_gap    1998..2078
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2249..2674)
                     /locus_tag="PK977_05985"
     CDS             complement(2249..2674)
                     /locus_tag="PK977_05985"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF17695.1"
                     /translation="MENDGRIYQYVGDEIVVWWPESKANAQKAVAALLTARKELHKQF
                     NRFKRKYDTLPEYKAGIHTGTVTVGQVGIEKKDLVMSGDAINTAARIRSACTELNQKY
                     IASKDVVDLLDLKEWQTESLGLIDLKGKNQGMELFVLKI"
     gene            complement(2741..>3379)
                     /locus_tag="PK977_05990"
     CDS             complement(2741..>3379)
                     /locus_tag="PK977_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17696.1"
                     /translation="TSLLYTSHMLKANRSILPRVSPIKRHQRKVILGIALLWTTIDFI
                     FFLWQKFIGVLPDKYYKPDINFTKEVLVREVSVFIISLFIGYFLVYVLRNYLRKSSLW
                     VNLLVKTLLLVLVAFVMTFFVYMTYEWLIAGRTIEAALEKFQYNLLHKRLFVQKMPEW
                     VILFFFTLLAIEVNEKYSRGVFLNIMLGRYLQPREERRIILFLDLKDSTPIA"
     assembly_gap    3380..3482
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3483..3815)
                     /locus_tag="PK977_05995"
     CDS             complement(<3483..3815)
                     /locus_tag="PK977_05995"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRF17697.1"
                     /translation="MPPKKKPASEKIKSVKPSGSRDENFFSLVYEVARQIPKGRVTSY
                     GAIAACLGTKSSARMVGWAMNGCHRVRPKVPAHRVVNRNGMLTGKHHFSPPGLMEQLL
                     KKEGINVKN"
     gene            complement(4039..4230)
                     /locus_tag="PK977_06000"
     CDS             complement(4039..4230)
                     /locus_tag="PK977_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HRF17698.1"
                     /translation="MKDLIEGIHYYINEDGLIVLTEKYHLEKGYCCGNGCRHCPFEYE
                     NVPEPKRSELLEQKNSKEK"
     gene            complement(4214..4924)
                     /gene="trmB"
                     /locus_tag="PK977_06005"
     CDS             complement(4214..4924)
                     /gene="trmB"
                     /locus_tag="PK977_06005"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRF17699.1"
                     /translation="MGQKKLQRFAELETFSNVLQFPKDIKGKWKTVFHNDHPITLELA
                     CGKGEYAVGLGRLYPNRNFIGIDLKGNRIWVGAKKALQENLSNVAFLRTQIDQVTEYF
                     DAKEVAEIWITFPDPQLRLSKAKKRLTHPKFLRLYQQFLAPGGLIHLKTDSPDLYRFT
                     KLVIELYGCPLHTDMDDVFNNETVSEELKIKTHYESLDIAGSNRIHYICFSLPGDLGN
                     KEKDLALKEKLQAHEGSH"
     gene            complement(4941..5867)
                     /locus_tag="PK977_06010"
     CDS             complement(4941..5867)
                     /locus_tag="PK977_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRF17700.1"
                     /translation="MKRLILFYCLISLSFAATAQDAIIKKLQAESLRQVKKDVPDTAW
                     NWKTGGAFGINVGQGSQSNWAAGGDDFSFTLNTSLRLFAFYKQNNHSWDNTFDVNFGY
                     INTTSLGGRKNDDRFDFLTKYGLALNKKLNLATLANFRSQFLKGYTYPNNVKTFASAF
                     LSPAYLIASQGLDYKPAKELSIFLSPVTSRWVFVRDSILSAKGEYGVKQHTHSINQNG
                     AFATISYQKSFNKYISYKSRLDLFSNYKKDPQNVDIFMTNTFSAKVAKIIAFTWNVDM
                     IYDDDVRLFGEEGSSPGLQFKSVVAVGLQVKI"
     gene            complement(6078..>6351)
                     /locus_tag="PK977_06015"
     CDS             complement(6078..>6351)
                     /locus_tag="PK977_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRF17701.1"
                     /translation="AFATINYLKEISKNITYKGRLDLFSNYRHNPENIDVFMSNILNA
                     KLGKVLSVSWSVDKIYDDDVKLFGTNKNRPALQLKSLVGIGLLLKF"
     assembly_gap    6352..6370
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6371..7015)
                     /locus_tag="PK977_06020"
     CDS             complement(<6371..7015)
                     /locus_tag="PK977_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRF17702.1"
                     /translation="MKKIIIVLIIISCSTVNAQDQTVKALQNEAGKAIKKDASDTSKK
                     NWKKGGIYSLNISQGSLSNWAAGGDDFSLSINSLLSLYAFYKKDKNSWDNTFDFNLGY
                     VNTTSLGSRKNDDRFDLLSKYGYALNPKLNLSALFNLRSQFFKGYTYANNVATYSSNF
                     MAPGYLLLSAGLDYKPTKNLSIFFSPITTRWVIVRDTALSNKGAYGVTPGKKTNL"
     gene            complement(7093..>7427)
                     /locus_tag="PK977_06025"
     CDS             complement(7093..>7427)
                     /locus_tag="PK977_06025"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRF17703.1"
                     /translation="ALPQTITQLKRLKYLALSYNKFMTLPASITGLPNLTVLDLNNNK
                     LMELPQDIGKLNALQELYLASNKLKTLPESITQLQNLQTINLRDNSSLKLTDAQKTFI
                     EDKKLHTP"
     assembly_gap    7428..7450
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7451..7988)
                     /locus_tag="PK977_06030"
     CDS             complement(<7451..7988)
                     /locus_tag="PK977_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRF17704.1"
                     /translation="MKKLTLLILSALLINTAHSQKDKVYSSLEDALQNPAEVYILSLA
                     DKKLSSVPADVFKFPNLVTLDLSYNKISTLPENITQLKSLTELNLSGNKLSNLPAAVT
                     KMTSLAELDLSDNNLSALPASFSNLTSLKVLYLTNNKFKAIPDAIFKLSSLTELYIYG
                     NMLASLTESIGKLTNLAEL"
     gene            8552..9016
                     /locus_tag="PK977_06035"
     CDS             8552..9016
                     /locus_tag="PK977_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRF17705.1"
                     /translation="MAVKLNLDKLDLQIIHHMMENAEISYADLGKKLFVSGGTIHVRI
                     KKLQEAGVVKGTRLKADLKQLGYDVIAFIGIYLKESSLYDSVAKELQKIPEIVRLNYT
                     TGNYSMFAEIVCKDITQLRWVLHDQLQKIKGIERTETFISLEESFSRNVKVI"
     gene            9031..10470
                     /locus_tag="PK977_06040"
     CDS             9031..10470
                     /locus_tag="PK977_06040"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRF17706.1"
                     /translation="MKESRHFYSSLGLLLLLNILIKPVWIFGIDRQVQNVVGLSVYGT
                     YFSLLNFSIVFSFLLDWGFTGFFNRQLAAQPEAFHAIVGKLIYLKLLFILLYVVVVIL
                     AGWLTGVDHWIILIAVIIIQSLTSLFVFLRGIITAKQWFRTDAWLSILDKGLMILICS
                     GFFLWPALTGSMTINSFLWIQVLCSAVAVLAAAVLVFMKEGLFTTPSPVINRSILRQA
                     LPFALIVLLMSAHYRLDGFMLERMHSDGARQAGIYAAAYRLLDAGNMIGYLTASFLLP
                     FVARHQNNGPVTGAVVNNSRHLLLVFSIAVSVTVFFLADFIQSILYHSADPYVSTVLQ
                     WCLPALIGYSLVQVYGTVMTATGNIGSFCVIVLASLVVNVILNILLIPSLGAKGCCIA
                     AVISHGLCGITSMLFTTQKSGLGLNLRSLLIYTFIAAVLAAYFYAGKRAELNEWLLLA
                     GSIAVEIALLMAAGLFSIRKWKLYLQQNQ"
     gene            10480..11289
                     /locus_tag="PK977_06045"
     CDS             10480..11289
                     /locus_tag="PK977_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17707.1"
                     /translation="MPDMFDLFNHWWKQMLAVIIASLLIVGTVTYLKPKKYLSVATAV
                     PASSFASDKSKIFNENIQALYSALGTPDDLDMIVGTARLDTVYLAVTDQFNLYDHFKM
                     QEKGEAARSKAASLLKKNTKVMKSEYGELKVKAWDTDKNLAPQLANAIMEKLKAIHQD
                     LQNAGNLATLQGLISRKEKLQQAADSTMNTVENSNQQKEQISQYEKLIGEYQLMVDTK
                     PPVLITVEKAKAAAWPDQPKTVQLLMATVVLAFIFSLLAALVLERKKTAGQ"
     gene            11473..12723
                     /locus_tag="PK977_06050"
     CDS             11473..12723
                     /locus_tag="PK977_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRF17708.1"
                     /translation="MEYNFSSGLGTDLPDEPLMLLTSFLFLAYWMYKPASLPKKMIQH
                     PLLLLLILLLAWTTVTVFFSTDKVVSIKYLLAKSWYIGAFVLCPLILFQERKLFIIAM
                     ILLTAAMLVVTLVSLYRHYLYGFSFAGINDALTPFFRNHVNYSAMLVCFVPLLIAFYK
                     LTKKQHLRLLTGAALSVVLIALFFSYARGAWLALLAGLFTYWLIQHRLLIYAYIIALI
                     VVITSIFWLKSGDRYLLYAHDYKTTIFHKDFNEHLVATYKLKDVSTAERFYRWIAGVR
                     MIKDNWLTGYGPNTFYGNYKPYAVPAYKTWVSDNQDRSTVHNYFLLTIIEQGIPGLIL
                     LLLLVGTVFYYAQHLYHRVKDRLYKVTAITTGAILTMLMVVNFLSDLIETDKVGSLFF
                     LCIAVLVVVDLNTRQESSEPATDI"
BASE COUNT         3419 a         2499 c         2814 g         3866 t
ORIGIN      
        1 gaaaagattc ttgtttttaa gttttggaaa gtcgatgtcg aattgtacgg cataagttac
       61 atcttcggtg aaggcttgag tttttatgtc ttcaacatga acttttaaat tgtctcctga
      121 aattgcatac gttggttttt ccaaaacctc taaaaatcgt ttgtcaatgt ggtttttgaa
      181 attttcaatt agcttctcaa ctgtttcagt gttttcttcg gttttttctt gttcttcaaa
      241 tgttattttt gaaatttccg tcacaagttt ttctataaca acttttgaca tttcttcgtc
      301 aaacttaatt tcaaaaagtg caagtagttt agaaaaaaac tcaacaactg aacgataatc
      361 gtttaattgg tagcattgat acaaacctaa tggcggtagt atgctgtctc gatttatacc
      421 gatacaaaca ggtattactt cacaattttc aagagcttgt ccaattccac tttcaaaata
      481 tacccatggt ctgttaacac tatttggtgt caacaatgaa accacggctt tactttgagt
      541 aatttttgaa agtatttggt taaaccaaat atctccaggt ttaagtccgc ttcctcctgt
      601 cgaatcagat gaaaaccaag ggctgatttg ttcaagtgta attcgtctta gggctcctga
      661 aaataattca gcaattgcct tgtctccgct gtaatgactt ataaaaaatt tatgtggcat
      721 aatcgtagag tgtcaaataa attaccgcta actcataaat atacgcaact gctggtacag
      781 ttttctattg gtagtcagtg attttttgta ccagctgagg tgcagcaata caaaatttaa
      841 tatccgtgta taaacgttta gaaacgtttg tacacgacta tttcctgtca tctcaaataa
      901 atgttcgtca attgttttat agggaaatta gtaaactgac cccacccccg caataccctt
      961 tttgggttat ttcaacaggg gcaaggagac tggcatagca aaaaaggcac tgtaaactta
     1021 aaccagtatt aatccccaca actgtaaagt tttttctgta caagacaatc tatcaatgcc
     1081 gctatgactc cataacaact cccgcctaac taccacccaa agccatggta actccatcag
     1141 tctgttccca agcagccacc agtaccatac catgattttg tagattaagc ccggggccgg
     1201 ctcattgtgt aaacgctttc aaaattcttc cccggatatc agtaatcctg atatggcctg
     1261 ggtggccgcc ggaaagtatc ttgtcgcctt tattattcca ccccacaccc cacagattgt
     1321 tgttagctaa ccgggcctta tggatgagtt tcccttttca ttacttcaaa acatcaatag
     1381 aataatgttg cgtaaacttt tgccaatgac tgccattcat cactatctcg catacgccac
     1441 ataactgcgc ccaccaaaaa gacaataaac acaacaagaa agggtttata atttttcttt
     1501 ttgtaaagat catttcccag caggcaaccg acaatggcca ggttaaccaa atccaacgtt
     1561 ttgaaaacag tatcgaagtc aaggttgaaa ctgttgacgg ccaatgcccg gcccgatagc
     1621 taataaacct gtggcgatca tgtaccgcat atgtgaggcg gggtgtttac gaaagagcat
     1681 ggccagtgtc caaaaaatgg caaatgaaaa taagcctctc gaatccagca ctataaactt
     1741 caatgctgcc tgctcattag cgttttccat aattcgccag taggattctc ttcccgcaag
     1801 gaacaggaac aggattaaaa gcggcccatg aacataagct actttaccaa tagcccgatg
     1861 aagctgcggc cttccagtat agataagaaa aggctgcact accagtaaag ccagccacgt
     1921 catccacaaa gctccatgta tatgtataat agtggtttta tttatgaaat tggggaactg
     1981 gctgatgtag tatttatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ttgttactga aagttaacta
     2101 attcaattaa ttggtctcac tttcctggct aacaaaacag acgaagttta gggcttttaa
     2161 aaaagctggc tcaacaatta aattttcact actacaggca ttagtttctt ttgataccag
     2221 gttgaataaa ttccagccat gaacccttct atatcttcag cacaaacaat tccattccct
     2281 ggttttttcc tttgaggtcg ataagaccga gtgattcggt ttgccattct ttgagatcga
     2341 gcaggtctac cacatctttg gaggcgatat atttttgatt gagctcggtg caggcgctgc
     2401 gtatgcgggc agccgtattg atggcatcgc cgctcatgac aaggtctttc ttttcgatac
     2461 ctacctggcc tacggtaacg gtgccggtgt gtatgccggc tttgtactcg ggcagtgtat
     2521 catattttct tttgaagcgg ttgaactgct tgtgcaattc tttgcgggcg gtgagcaggg
     2581 cggctacggc tttttgtgca ttggctttgg actcgggcca ccataccaca atctcatcac
     2641 ctacatactg gtaaatgcgt ccgtcgtttt ccataaaacc ggaagagatg cagaagataa
     2701 agtcgctgat gaatttgaaa tattctttgt gaccgagttt ttaggcaatg ggagtggagt
     2761 cctttaaatc gagaaagagg atgatccttc tttcttcccg cggctgcagg tagcggccga
     2821 gcataatgtt gagaaagacg cctctagaat atttttcgtt gacctctata gccagcaggg
     2881 tgaagaagaa gagaatgacc cactcgggca ttttttgtac aaataatctt ttgtgcagca
     2941 ggttgtactg aaatttttca agtgctgctt ctatggtgcg tccggcgata agccattcat
     3001 aggtcatgta cacaaagaag gtcatgacaa aagctaccag caccagcagc agggttttca
     3061 ccagcaggtt cacccagagg gaagatttgc gcaggtaatt gcgcagcaca tataccagga
     3121 agtaaccgat gaaaagactg atgatgaaga cacttacttc ccgtaccaat acttctttgg
     3181 tgaaattgat atcgggctta tagtatttgt cgggcagtac tccaatgaac ttctgccaga
     3241 gaaagaagat aaagtcgata gtggtccaga gcagtgcaat gccgagtatg acttttcgct
     3301 ggtgtctttt gatgggtgat acccggggaa gtatggagcg gttggctttt agcatgtggc
     3361 tggtatatag caggctggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnattcttta cgttgatgcc ttcttttttt aataattgct ccatgaggcc gggaggggag
     3541 aagtgatgtt taccggtgag catgccatta cggttgacta cacgatgtgc cggtactttg
     3601 ggccttaccc ggtgacaacc gttcatggcc cagcctacca tgcgggcgga tgatttggta
     3661 ccgagacagg cggcgatagc tccataggaa gtgaccctgc ctttgggaat ttggcgggct
     3721 acttcgtaga caagagaaaa gaaattttca tccctgctgc cggagggttt cactgatttt
     3781 attttttcgg aggccggttt ctttttagga ggcatagttt tttgtttccc gctaggccac
     3841 taaggtcaca aagttttttg ataaaagagg gatggttggc cacggattac acggattaac
     3901 acggatgatt tttttgaata gtcattagta tcaatgtgca ggtacaaaac aataattgat
     3961 tgctaagcta ttaatctgtg aaaatctgtg taatccgtgg ctaatgtatt ttgtgttctt
     4021 cgtggcttgg tgggaaacct acttctcttt tgaatttttc tgttctagta attcactgcg
     4081 tttcggctcg ggtacattct cgtattcaaa aggacaatgc ctgcagccat tgccacagca
     4141 ataacctttc tccaggtggt atttttctgt gagaacgatc agcccgtctt cgttaatgta
     4201 ataatgtatg ccttcaatga gatccttcat gtgcttgcag cttttctttc aatgccaggt
     4261 ctttttcttt attgcccaaa tcacccggca atgaaaagca gatgtaatgg atgcggttgc
     4321 taccggcaat atctaatgat tcgtaatgtg tttttatttt caattcttcg ctgacagttt
     4381 cgttgttgaa tacatcatcc atatcagtat gcagcgggca cccgtacagt tcaatcacca
     4441 gtttagtaaa gcggtacagg tccgggctgt cggtctttag gtgtattaaa ccaccgggag
     4501 caaggaattg ttgatacagg cggagaaatt ttggatgcgt aagccttttt ttggctttag
     4561 ataaacgtaa ttgcgggtcc gggaaagtga tccatatttc tgcaacttcc tttgcatcaa
     4621 agtattcagt tacctggtcg atctgggtac gtagaaaggc gacattactt aaattttcct
     4681 gcaaagcttt tttagcgcct acccaaatgc ggtttccttt caggtcgata ccaataaaat
     4741 ttcgattcgg gtacaggcgg ccaagcccta cagcatattc acctttgcca caggccagct
     4801 caagggtaat gggatgatca ttatgaaaaa cagttttcca tttccctttt atgtctttgg
     4861 gaaattgcaa tacgttggaa aaagtctcca gctctgcgaa gcgttgtaat tttttctggc
     4921 ccatggcggc caaaggtaac ttaaattttt acctgcagcc cgacggccac taccgactta
     4981 aactgtaaac cgggcgaaga accttcttcg ccaaagaggc gcacatcatc atcatagatc
     5041 atatctacgt tccaggtaaa ggcaatgatc ttcgctactt tggcagagaa agtattcgtc
     5101 atgaatatgt cgacattctg cggatctttt ttgtaattgg agaaaagatc cagcctgctc
     5161 ttgtaggaaa tatatttatt gaatgacttc tggtaactga tggtagcaaa agcgccgttc
     5221 tggttgatgc tatgcgtatg ttgttttacc ccatattcac ctttggcaga taagatgctg
     5281 tctcttacaa atacccagcg ggaagtaacg ggtgagagaa agatggataa ttctttggcc
     5341 ggtttataat ccagtccctg gctggcaata aggtaagccg gtgaaagaaa tgcgcttgca
     5401 aatgttttta cattattggg ataggtatag cctttcaaaa actgggagcg gaaattggcc
     5461 agtgtggcaa ggtttagttt tttgttaagc gccaaaccat acttggtcag aaaatcaaaa
     5521 cggtcatcgt tcttgcggcc gcccaaactg gtggtgttga tatagccaaa gttgacatcg
     5581 aatgtattat cccagctatg gttattttgt ttgtagaaag caaacaggcg cagcgaggtg
     5641 ttaagggtga atgagaaatc atcaccacct gccgcccagt tgctctggct gccctggcct
     5701 acgttgatgc caaaggcacc gccggttttc cagttccagg ctgtgtcagg aacatccttc
     5761 tttacctggc gcaacgattc ggcctgcaat tttttaataa tagcatcctg tgccgtagca
     5821 gcaaatgata aactgatcaa acaataaaac agaatgagcc tcttcatgag aaatagtccg
     5881 gtttattcag taatagtggt tgaatggatg gtagtcttca gcagggtgcc gcaaagatat
     5941 accgggcaat aaaccggaga acaggttttt tattttatag gttatgttaa aacaccagtg
     6001 gtacgaatgg gtgtttgtat aaaagaaaaa ccacattcgc ttgtgcatga atgtggtttt
     6061 gatatatgta taaacactta gaactttagc agcaggccaa tacccaccaa tgattttaat
     6121 tgcaaggcag gtctgttctt attggtgcca aacagtttta catcatcatc gtagatctta
     6181 tctacactcc atgataccga taatactttg cccagttttg cattcaggat gttggacatg
     6241 aacacatcaa tattctccgg gttgtgccgg tagttggaga aaaggtccag ccttccttta
     6301 taagtgatgt tcttgctgat ctctttcagg taattgatgg tggcaaatgc annnnnnnnn
     6361 nnnnnnnnnn caggtttgtc tttttaccgg gtgtaacacc ataagctcct ttattggaaa
     6421 gcgccgtatc cctgacgatc acccaacggg tagtgatagg tgaaaagaag atggaaagat
     6481 ttttcgtggg tttataatcc agaccggcac tcaataaaag atatcccggc gccataaagt
     6541 tggatgaata ggtggccacg ttgttggcgt aggtatatcc cttaaaaaac tgcgaacgaa
     6601 ggttgaataa agcagaaagg ttgagcttgg gattcaaagc ataaccatat ttggaaagca
     6661 ggtcaaagcg gtcatcattt ttccggctgc ccaggctggt ggtgtttaca taaccgaggt
     6721 tgaagtcaaa tgtattgtcc cagctgtttt tatctttttt ataaaaagca taaaggctga
     6781 gcaaggagtt aatcgataag gaaaaatcat cgccaccggc tgcccagtta cttaaggaac
     6841 cctgtgaaat atttagacta taaatgccgc cttttttcca gtttttctta ctggtgtcgc
     6901 tggcatcttt tttaatggct tttccggctt cgttttgcag ggcttttacg gtttgatcct
     6961 gtgcattaac agtgctgcat gatataatga tcaggactat aataattttt ttcattgtaa
     7021 tcacttttat ccgtaaaggt aaaaccggtg ctgttcttgt caactgattt tcgacagatg
     7081 agtagaaggg ctttatggcg tatgcagttt cttgtcttcg ataaatgttt tttgtgcatc
     7141 agtgagttta agactggaat tatcccgcag gttgatggtt tgcagatttt gcagttgcgt
     7201 aatactctcc ggcaatgttt tcagtttgtt agaagcaagg tataattcct gcaatgcgtt
     7261 gagttttccg atgtcttgcg gcagttccat cagcttattg ttgttcaggt caagtacggt
     7321 aaggttaggc agaccggtga tagatgcagg aagcgtcatg aacttgttgt aggaaagggc
     7381 caggtacttc agtcgtttta attgggtgat ggtttgtggc agggcaannn nnnnnnnnnn
     7441 nnnnnnnnnn ccagttcggc gaggttggtt aattttccaa tactctccgt cagggaggcc
     7501 agcatattgc catagatata caattcagtc aatgagctga gcttaaaaat ggcatcgggt
     7561 attgctttga atttgttatt ggttaagtaa agcaccttca ggctggtaag attggaaaaa
     7621 ctggcgggaa gtgcagagag attgttatcg ctgaggtcca gttcggcaag gcttgtcatt
     7681 tttgttactg ctgcaggaag attactcagc ttattgccgc tgaggtttag ttctgtcagt
     7741 gacttcagtt gcgtaatgtt ttccggcagg gtggaaatct tattgtatga caggtcaagg
     7801 gtaaccaggt tcgggaattt gaaaacatca gccgggacag aagacagttt tttatctgcc
     7861 agtgaaagaa tatatacttc tgcaggattt tgaagggcat cttccaggga agagtacact
     7921 ttatcttttt gtgaatgagc ggtgttgatc agcagagctg aaaggataag cagggttagt
     7981 tttttcatgc actaaatttc ggttatttct gagggatggc aataaccggg ctgattataa
     8041 agtgaagtga aaaaaggcca tacctgtttt aaagaagggt ttaagaattt gtagtagccc
     8101 cgatttacat cggggtatag agcagttatg tgcccgggct tgatgtaata gtattcatta
     8161 attgatataa aagaaaagcc tcccctgatc aagagaaggc tttttagctg tagggggagg
     8221 gttcgaacct ccacggagca gttagcgtct gcacaaatta gaagagagag ctactctcaa
     8281 acaactggtg gtcaacccca gtcggtccgc ccgtcggcgg atcggcgtta tgcagtgttt
     8341 atcctgtgat ctccaccccc gagacaagag ggcatgtctg ccatgctttc gcaatttcat
     8401 caccccacag tgtgttttaa agaacttgat agaacaaatg taggccagat cgccgtttca
     8461 ttccaaattt ttcaataatt attttaaata tttagatctt attcaaataa tattttatta
     8521 ttgtgaataa ttttcaaaat caatagaaaa aatggcggta aaattgaatt tggacaaact
     8581 ggatctgcag atcatccatc atatgatgga gaatgcagag atatcttacg ctgatctggg
     8641 taaaaaactc tttgtttccg gcggcaccat ccatgtccgc atcaaaaagc tacaggaagc
     8701 cggagtggtt aaaggtacaa ggttgaaggc agacctgaag cagctcggtt atgatgtaat
     8761 tgccttcatc ggtatctacc tgaaagaaag ttctctttat gattcggtgg ccaaagaact
     8821 gcaaaagata cccgagatcg tccgcctgaa ttatacaacc gggaattaca gcatgtttgc
     8881 cgagattgtt tgtaaagaca taacacagtt gcgctgggta ttgcatgacc agttgcaaaa
     8941 gatcaaagga attgaacgaa cagagacatt tatttcattg gaagagagct ttagcagaaa
     9001 tgtgaaggtg atctgatttt cagtttacgt atgaaagagt cccgccattt ttattcttcc
     9061 ttaggactgc ttttactcct gaatattctt atcaagccgg tttggatatt tgggatagac
     9121 aggcaggtac agaatgtggt gggcctctct gtttacggaa cctatttctc tctcctgaac
     9181 ttctccattg ttttcagctt tttactcgac tggggattta ccggtttttt taatcgtcag
     9241 ctggcggcgc aaccggaagc atttcatgct attgtcggta aattgattta tctaaaactg
     9301 ctttttattc tcttatatgt tgtagtagta atattggctg gatggctaac aggcgttgat
     9361 cattggataa tactgatcgc tgttattatt attcagtcgc ttacttcttt gtttgttttc
     9421 ctgcggggaa taataactgc taaacaatgg ttccgtaccg atgcctggtt gtcaattttg
     9481 gataaagggc tgatgatact aatatgtagc ggattttttt tgtggcctgc cttaacaggc
     9541 agcatgacca tcaatagctt tttgtggata caggtgttgt gcagtgctgt agctgtactg
     9601 gccgcggcag tcctcgtatt tatgaaagaa ggattgttta ccaccccaag cccggtgatt
     9661 aaccgttcaa tactccggca agcactaccc tttgcattga ttgtattatt aatgtcggcc
     9721 cattaccgcc tggatggttt tatgctggaa agaatgcact ccgatggtgc ccggcaggcg
     9781 ggaatatatg cagctgcgta ccggctgctt gatgccggta atatgatcgg ctacctgaca
     9841 gcatcttttt tattgccatt tgtggcccgt catcaaaata acggacctgt aaccggagcg
     9901 gtagtgaaca atagccgtca tctgttgctg gtgttttcga tagctgtatc agttactgtt
     9961 ttcttcctgg cagattttat tcaatcaata ttatatcact ctgctgatcc ttatgtttca
    10021 actgtattgc aatggtgcct gccagcactg atcggttatt cattggtgca ggtgtatggg
    10081 acagtcatga cagctacagg taatatcggt tcattttgtg tgatagtgct ggcttcgctg
    10141 gtagtaaatg tgatactgaa tattctgctg ataccttctc tcggtgcaaa gggttgttgt
    10201 atagcagcgg ttatcagtca cggactgtgc ggaattacca gcatgctgtt tacaactcaa
    10261 aaatcaggac tgggtttgaa tctccgctca ctgttaatct atacttttat agcagcagtg
    10321 ctggcggctt atttttatgc cgggaaaagg gctgaactca atgaatggct gttactcgca
    10381 ggatcaatag cggtggaaat cgcactgtta atggctgccg gtcttttttc cattcgcaaa
    10441 tggaaactgt atcttcagca aaatcaataa ataattttta tgcccgatat gtttgacctt
    10501 tttaatcatt ggtggaagca aatgctggca gtgataattg cctccttgct tatagtggga
    10561 acagttactt acctgaaacc caaaaaatac ctttcggtgg ctacggcagt acctgccagt
    10621 tcttttgcat ccgacaagtc gaaaatcttt aatgaaaaca tacaggccct ttattccgct
    10681 ctcggtacac cggatgacct ggatatgatt gttggtactg ccaggctgga tactgtttac
    10741 ctggcggtta ctgatcaatt caatttgtat gatcatttta aaatgcagga aaaaggggaa
    10801 gctgcacgga gcaaagctgc ttcgttgctg aagaagaata caaaagtgat gaagagtgaa
    10861 tacggcgaac tgaaggtaaa agcatgggat actgacaaaa acctggcacc gcaactggcc
    10921 aatgccataa tggagaaact gaaagccatt caccaggacc tgcagaacgc aggcaatctc
    10981 gccacattac agggattgat aagcagaaaa gaaaaattgc agcaggcggc agacagtaca
    11041 atgaatacag ttgagaatag caatcaacaa aaagaacaga tcagccaata tgaaaaactg
    11101 attggtgaat accagttaat ggttgacacc aaaccaccgg tactcattac ggtagaaaaa
    11161 gcaaaagctg cggcatggcc cgatcaacct aaaacagttc aactacttat ggcaacggtt
    11221 gttttggcat ttattttctc actcctcgct gcactggtgc tggaaagaaa aaaaacagcc
    11281 ggtcagtgaa cagtgttttt cataacagga atattctttt tacagggctg gctgcactat
    11341 ttcttatagc tgcaggcttc tctgtttata cgggtaatta tctgatagca attgcgccct
    11401 ttcttttttt atgcaggctg gcaactcaag gagctggttt ttttccttct gattgtttca
    11461 ctgccttttt cggtggaata taatttttcg tctggactgg gtacagacct gcccgatgaa
    11521 ccattgatgc tgctcacttc gttcctgttc ctggcctatt ggatgtacaa accggcctca
    11581 ctgccgaaga aaatgataca gcaccccctg ttactattat tgatattgtt actggcatgg
    11641 acaacagtaa cagtattttt ttctactgac aaggttgttt ctattaaata tcttttagcc
    11701 aagagctggt atatcggcgc ttttgtttta tgtcctttaa tcctgtttca ggaaagaaaa
    11761 ttatttatta tagcaatgat actgttaaca gcagccatgc tggtggttac attggtatcg
    11821 ttgtacaggc attatttgta tggcttcagt tttgcaggta tcaatgatgc attgacacct
    11881 tttttccgaa accatgtgaa ctactcagcc atgctggtat gttttgtacc actattaatt
    11941 gctttttata agctgactaa aaagcagcat ctcaggttat tgaccggggc tgcattatca
    12001 gtcgttttaa ttgctctttt cttttcgtat gcaagaggcg cctggctggc gttactggca
    12061 ggtttgttta catattggct gatacagcat cggctgctca tctatgctta tatcattgca
    12121 ttaattgttg tgattacttc catattctgg ttaaaaagcg gtgaccgtta tttactttat
    12181 gctcatgact ataagaccac tatttttcat aaagatttta acgagcattt ggtagctact
    12241 tacaagctga aagatgtatc tactgccgaa cgattctata gatggattgc aggagtcagg
    12301 atgataaagg ataactggct gaccggttat ggccccaata ccttttatgg caattataaa
    12361 ccgtacgccg taccggcata taaaacatgg gtgagcgata atcaagaccg ctctacagta
    12421 cacaattatt ttttgctgac tataattgaa cagggaattc ccgggcttat cctcctgttg
    12481 ttactggtgg gaacagtttt ttattatgca caacacctgt atcatcgggt aaaagatcgt
    12541 ttatacaagg tcactgctat aacaacagga gctatactta ctatgctgat ggtggtgaat
    12601 tttttaagcg acctgattga gacagataag gtgggtagcc ttttttttct ttgtattgct
    12661 gtattggttg tagttgatct gaacacccgc caggaatcat cagagcctgc cacggacatt
    12721 tagggcatct cttaatccat ttcctagcaa attaaatgca agtaccaaaa tcattatcgc
    12781 aaatcccggc gctaaagcaa gcatgggatt ttgagtgatg atga
//
LOCUS       DAORLS010000054        12540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2675_length_12540_cov_1.953384, whole genome shotgun sequence.
ACCESSION   DAORLS010000054 DAORLS010000000
VERSION     DAORLS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_12540_cov_1.953384"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1194
                     /locus_tag="PK977_06055"
     CDS             <1..>1194
                     /locus_tag="PK977_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF17709.1"
                     /translation="YGQPIALVVADTWEKAVKAAKLVKAEYFTEPFETDFEKLRKYES
                     KLKKISEYKRGETDAYKNADVFVEAEFSTPIETHNPMEMHATIAIWEGDDKLTVYDKS
                     QGPKGTQSQLARCFGLEEKNVRVITEFVGGAFGASLRSWPNVPAAVIAAKKVKRPVKV
                     LLNRRQMFSMVGYRPQAWQKIAIGANKDGKLVGITHHAISNTSRYEDYREGIVNASQF
                     LYDCPNVNTSYKILPLDLNTPTWMRGPGEATGAFALECVLDELAYKLNMDPVELRVKN
                     YAELHPVSKLPWSSKFLKECYDAGREKIGWDKRQATPRSLKEDGMLAGYGMGGGVFGA
                     GRGNAAVKAILKRDGSLILQSAVSDMGPGTATTMVKIAETSMQMPASKIRFMLGDTDF
                     PPGPT"
     assembly_gap    1195..1254
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1255..>1574
                     /locus_tag="PK977_06060"
     CDS             <1255..>1574
                     /locus_tag="PK977_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF17710.1"
                     /translation="KKHLKELAIANVASFKGSSIDDIEIKNEQLISKKDNARRISLSE
                     LVSSSGKEQVEIIQESGRAAAEAQKYAMNSFSAHFVKVHVDPSSGTVKLKKVIATGDG
                     GKII"
     assembly_gap    1575..1634
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1635..1910
                     /locus_tag="PK977_06065"
     CDS             <1635..1910
                     /locus_tag="PK977_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRF17711.1"
                     /translation="MALTEEVLIDHSTGKYINDNLGSYHVPLHTDVPHIDAWFPNKPD
                     PVINATGAKGVGEIALVGFAAAVANAVFNATGKRIYKLPIKPEDLIS"
     assembly_gap    1966..2002
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2003..3305)
                     /gene="glmM"
                     /locus_tag="PK977_06070"
     CDS             complement(<2003..3305)
                     /gene="glmM"
                     /locus_tag="PK977_06070"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRF17712.1"
                     /translation="MALIKSISGIRGTIGGKPGDNLTPVDIVKFAAAFGTIIAKDKPA
                     TKIVIGRDGRISGSMVQELVVNTLTGLGINVVDLGLSTTPTVEVAVKMEKADGGIILT
                     ASHNPKEWNALKLLNEEGEFISAETGANVLELAAREDFLFAFVDRLGTVTKDDSYLQK
                     HIDAILKYPLVNAKAIAKQHFKVVVDAINSTGAIYVPALLKALGVTEVIVLNGEVNGK
                     FAHNPEPLPEHLSQLCNEVVQQKAHLGIAVDPDVDRLCFVNEDGTLFGEEYTLVAVAD
                     YVLGKRKGNTVSNMSSTKALKEITQKHGGEYFPSAVGEVNVVNKMKEVNAVIGGEGNG
                     GIIVPDFHYGRDALIGIGLFLSHLADQKKGIKSLRSQYPDYFISKNKIELNNGIDVKA
                     IFEKIKKKYKKNPINTEDGLKIEFENDWVHLRTSNTEPIIRI"
     gene            3381..4187
                     /locus_tag="PK977_06075"
     CDS             3381..4187
                     /locus_tag="PK977_06075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF17713.1"
                     /translation="MSEKAWYKDWFNSPFYHKLYFERDEKEAENFIHQLVTHLKPLPG
                     SRMLDVACGKGRHSKTLAALGFTVTGIDISPDSIAYAKQFEKENLDFFVHDMRLPFWG
                     NYFDFAFNFFTSFGYFKTRREHDDAIRTIAKSLKPGGSFIIDYLNVHYAEEHLLHHET
                     KKIGSTAYEITRWDDETHFYKKISVTDPAVEGSIEYTEKVAKFNLGDFTDMLSFQGMQ
                     VNEVYGDYLFNPYDVRKTPRMIIIAEKKRNDKEGNKKRLYSDGRSTDALT"
     gene            complement(4184..6127)
                     /locus_tag="PK977_06080"
     CDS             complement(4184..6127)
                     /locus_tag="PK977_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRF17714.1"
                     /translation="MANLFVIFFFIFPLFRLITFFAFRPDGITFTETIPSFLMGARYD
                     LRWIAFVLLPIVITSLHPHLSPFYSSRNKKWWTWYLAIITFIIFFFFAAGFGSFSYNQ
                     TPLDAGAMNFAEDFSISWKMMWQTYPMILMLSGLVVAVILFRWMYHRSHWMVINRTDG
                     KGIPYRRKFFFIAILVLVFFAWGNLSWPPLGRNDSFRFKNSFKSYIAINPLQNFFATL
                     RLRKPDHNEAKAREVFPIMADWMQLPDKNNFTYRRQIAPRSNSFESRPNIVLVQCESF
                     SMYKSSMSGNPLNTTPFFDSLCRNGIFFEKCFAPHFSTARALFAILSGIPDAQLFKFS
                     SRNPDAVKQRTIINHFEDYEKHYFLGGSAEFSNFDGLLSNIKGLQMHTEENFKEPKVN
                     VWGISDKDLFLEANEVFKKKNSPFFAYIQTSGNHRPYNRTIPDSDTEFVRKTVPEEEL
                     KKYGFESLDEYNCFRYSDYCFRKFIEAAQKEAYFHNTIFVFVGDHGLAGNAGEMYPPV
                     WTEHRLTDEHVPLLFYAPDLLTPQRRKEVVSQIDVLPTIAGLLHQSYVNTTLGRDLLD
                     TDKKNNFAFITNTAGGIGMVTDDFYFTKNINFPDEELVPMHSSALTKYSKPQIDSIRK
                     ELSVFTHAFFETSKYLLMNNQKD"
     gene            6300..6905
                     /locus_tag="PK977_06085"
     CDS             6300..6905
                     /locus_tag="PK977_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF17715.1"
                     /translation="MFPLNLVFWPWLQRLDESLFVTINSQWTNPVFDSVMPFLRKSDH
                     WVPLYLFLLVFILMNFRTKAVWWIIFFILTVGITDYIGTNAFKYGFERIRPCNSANML
                     EHLRLLVPCPSGFGFTSNHAANHFGMATFLFLSFRHLFKNWVWLAYLWAGSIAYAQVY
                     VGVHYPMDIIAGAVWGLLVGTITGTVFNKWFGLKGDHLEYQ"
     gene            complement(6899..>7596)
                     /locus_tag="PK977_06090"
     CDS             complement(6899..>7596)
                     /locus_tag="PK977_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF17716.1"
                     /translation="DNHRPYTIPKEDRLYIKMRSFPKDTLDKYGFWTNEELNAYAYMD
                     YTYKVFMEAAAKEKYFNNTIFIFVGDHGIRGIAGDMMPKVWTDQSLTLQHVPMLFYAP
                     GKIQPQVIERKVSQVDIMPTIAGLINKPVTNSTMGRNIFALPPSSDSDYINNHAFVLD
                     FDRRLIGLVGGKYYYEYSLQTKGEGFYPLTHNNPLLPDEPSEKERSLIRQLTLGYYET
                     ARYMLFHNKKIAH"
     assembly_gap    7597..7691
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7692..8242)
                     /locus_tag="PK977_06095"
     CDS             complement(<7692..8242)
                     /locus_tag="PK977_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF17717.1"
                     /translation="MSTLKFRNVGYDEKAVRRHYNLMADVFGIKNRDTASLNFKRIMP
                     VQDSVPTIEKPNVVLVICESFSAYKSSMWGNPLNTTPYFNELCKQGAFFKNCFSPAYG
                     TARGIWAIITGIPDVTEFKTASRNPNLVDQHTILNDLKDYEKFYFLGGSASWANIRGV
                     LKDNIKGLHLYEQQDYSSPKIDV"
     assembly_gap    8251..8260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8591..8630
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8631..8942)
                     /locus_tag="PK977_06100"
     CDS             complement(<8631..8942)
                     /locus_tag="PK977_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17718.1"
                     /translation="MKKFIHSRLVRWMLLNGLFFLLLLTILRIIFHFVFTPRGEKAIG
                     DALWMGFRYDARVVCVFLMIVLLLSSIFYFYKPFTQVISRKILLVFTRLFGLLVIVVY
                     TF"
     gene            9177..>9423
                     /locus_tag="PK977_06105"
     CDS             9177..>9423
                     /locus_tag="PK977_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRF17719.1"
                     /translation="MSEIALVSARKGRLEHQAEKGDKKARLALEMSSHPELFLSAVQI
                     GITLISIVTGVYSGERFSKDLQPIVEKIETLRPYAEGI"
     assembly_gap    9424..9493
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9494..10438
                     /locus_tag="PK977_06110"
     CDS             <9494..10438
                     /locus_tag="PK977_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRF17720.1"
                     /translation="LIPKRIGLLRSEKIAKAVAGPMKFFARLFHPFVWLLNKTSNVFF
                     RVFNIKRIKDDAVTEEEIKTLITEGTEAGTIDEAEQEIIERVFHLGDRNITSLMTHRS
                     DIIWFNLDDSEDKIKEKILQEPHSSYPICDGSIDNIKGIVSIKDLYISPDSMLFKDIM
                     VPALFIPENNSPYQVMEKFKQSKIHSAFIVDEYGSILGLLTLNDILEAIVGDIPQPDV
                     PDYEIRKRDDGTYLVDGQIPFYDFLTRFEKTEWMNEGEHDFDTLAGFILHELERIPKT
                     GDKIDWKGFKIEVMDMDGHRIDKLLVTLSEELKEEMED"
     gene            complement(10444..>10830)
                     /locus_tag="PK977_06115"
     CDS             complement(10444..>10830)
                     /locus_tag="PK977_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17721.1"
                     /translation="TLEVTCKIFTDDFEGVLAKNYNTKVDLINPPNKAAMDTLVKKYI
                     ISHLAVKANGKPVKFSYLGFEHDKEAVFAYLEVENVQAVSAFTINNNLMYDFFDDQVN
                     IMHVFVGGNRKSTRLSYPDTEVTVNF"
     assembly_gap    10831..10898
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10899..11701
                     /locus_tag="PK977_06120"
     CDS             <10899..11701
                     /locus_tag="PK977_06120"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRF17722.1"
                     /translation="KPRITEFANLTEGSSFLIPSIKKADKSEKLNHLLSNEAIKTIIF
                     ICNFQGLIFRLCHQTLKIWFWFEPIDKISMSDFGLYFGIGWDHIMSWDALDHLLFVTA
                     LATIYYLKDWKQVLILVTAFTIGHSLTLVMSVLDIIRFPSNWVEFLIPCTIVITAFSN
                     LFQKKFTPKSIRINYFLALFFGLIHGLGFANTIRFMLASDQNIGWGLFAFNVGLEAGQ
                     LVVVAVFLLAAQAITSVFNIVRREWVIFLSAAVFSLSLQMVFQRISSL"
     gene            11716..>12540
                     /locus_tag="PK977_06125"
     CDS             11716..>12540
                     /locus_tag="PK977_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HRF17723.1"
                     /translation="MKRFTSAIALLLCFSLAGWSQNIQNNPSSNHGNKFEQMGSIFPT
                     PNEYRTASGAPGPKYWQQRCDYDIKCELDEKNLKLTGSETVTYFNNSPNELTYLWLQL
                     DENQHSSTKNANYQNSNPMSRQTSPQMLDRADEAKTDNGYGFNITKLTDAAGKDLKYT
                     INKTMMRVELPAVLKAGQKFTFKLDWNYKIADRMGRGGRGGYEYFAEDGNHLFTMTQW
                     YPRLCVYSDFKGWQNQQFTGRGEFALTFGNFKVQMTVPADHVIMSTGECQNYAAVLT"
BASE COUNT         3596 a         2560 c         2536 g         3408 t
ORIGIN      
        1 attatggcca gcctattgcc cttgttgttg ccgacacctg ggagaaggca gtaaaagcgg
       61 ccaaactggt gaaagcagaa tacttcacag agccattcga aactgatttt gaaaagttga
      121 gaaagtatga atcgaaactg aaaaagataa gtgaatataa aagaggtgaa acggatgcct
      181 ataagaatgc cgatgtattt gtagaagctg aattctctac gccaatagaa acacataatc
      241 ccatggagat gcatgctacc atcgccatct gggaaggaga tgataagctg acggtttacg
      301 ataaatcgca gggaccaaag ggcacacagt cacaactggc ccggtgtttt ggcctggagg
      361 aaaaaaatgt tcgtgtgata actgaatttg taggtggcgc tttcggtgca tcgctgagaa
      421 gctggcccaa tgtacctgct gctgttattg ctgctaaaaa agttaaaagg cctgtaaagg
      481 ttttactgaa tcgcaggcaa atgttttcca tggtgggcta ccggccacag gcatggcaga
      541 aaattgccat tggagccaac aaagacggta agctggtggg catcacacat catgccatca
      601 gtaatacatc ccggtacgag gattacaggg aaggtattgt aaatgcatcc cagtttttat
      661 acgactgccc gaatgtaaac accagttata aaatcctgcc tctcgatctg aatacaccta
      721 cctggatgcg gggacccggg gaagctaccg gtgcctttgc cctggaatgt gtattggatg
      781 aactggctta caagttaaat atggacccgg ttgaattgag ggttaagaac tatgctgaac
      841 tgcacccggt gagtaaactg ccctggtcga gtaaattcct caaagaatgt tatgatgccg
      901 gcagagaaaa aattggttgg gacaaaagac aggccacacc ccgttcattg aaagaagacg
      961 gcatgcttgc aggttatggc atgggtggtg gtgtgtttgg tgcaggcaga ggcaatgctg
     1021 cggtaaaagc tatactgaaa agagatggca gccttatttt acagagtgca gtaagtgata
     1081 tgggccccgg cactgctact accatggtaa aaattgcaga aacatctatg caaatgccgg
     1141 cttctaagat caggtttatg ctgggcgaca ctgatttccc tccgggaccg acacnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaaaa
     1261 acacctgaaa gaattggcca ttgctaatgt ggcttcattc aaaggatcct caattgatga
     1321 tattgaaata aaaaatgaac aactgatttc taaaaaggat aatgcaagaa gaatttcact
     1381 aagtgaactt gtaagttctt ctggcaaaga acaggttgaa attatccagg aatccggccg
     1441 tgccgcggct gaagcacaga aatatgctat gaattcattt tctgctcact ttgtaaaagt
     1501 gcatgttgat ccctcatcag gcactgttaa attgaaaaaa gtaattgcca ctggtgatgg
     1561 cggaaaaatc atcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnatggca ttgacggaag aggtgcttat tgaccacagc acaggaaaat
     1681 atatcaatga caatttaggc agctaccatg tgccgctgca tacagatgtg ccgcatattg
     1741 atgcctggtt tcccaataaa cctgaccctg ttattaacgc aacaggtgca aaaggtgtag
     1801 gtgaaattgc attagtggga tttgctgcag cagtagcaaa tgcggtattc aatgcaacag
     1861 gtaaacgaat ttacaagtta cccataaagc cggaagacct gattagttga tgatgggaaa
     1921 ttactggctg aactctttaa tatcccgcat cagctgcatc gcaatnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnatatgcgg atgataggtt ctgtattgga agtacgcaga
     2041 tgcacccagt cattttcaaa ttctatcttt agtccgtctt ctgtattgat ggggttcttc
     2101 ttgtacttct tctttatttt ctcaaaaatg gcttttacgt caatgccgtt gttcaattca
     2161 attttattct tggagataaa ataatccggg tactggctgc gcaaagattt gattcccttc
     2221 ttttgatcag ccaggtggct caggaataaa ccaatcccaa tcaaagcatc tcggccataa
     2281 tgaaaatcag gaacgatgat gccgccgttg ccctcaccgc caatcacagc attcacttct
     2341 ttcatcttat ttaccacatt cacctctcct actgctgacg ggaaatactc tccaccatgc
     2401 ttctgtgtaa tctctttcag cgccttggta ctgctcatat tgctcaccgt attgcctttt
     2461 cgcttgccaa gcacataatc ggccacggct accagtgtat attcttctcc gaataaagta
     2521 ccatcttcat tcacaaaaca aagcctgtca acatccggat ctacagcaat acccaggtgt
     2581 gctttctgct gcaccacctc attgcacaat tgagaaagat gttccggcaa aggttcgggg
     2641 ttatgggcaa attttccatt cacttctccg tttaaaacga tcacctcagt tactcccaat
     2701 gctttcagta aggccggcac atagatagct cctgttgaat tgatagcatc cactactact
     2761 ttgaagtgct gttttgcgat tgcttttgca ttcaccagcg gatactttaa aatagcatcg
     2821 atatgttttt gtaaataact atcgtctttg gttaccgtac caagtctgtc aacaaaagca
     2881 aaaagaaaat cctccctggc tgccagttcc agcacatttg cacccgtttc tgcagagata
     2941 aattcacctt cctcatttaa taactttaat gcattccatt ctttcggatt atgactggcc
     3001 gtgaggataa ttccgccgtc tgccttttcc attttcaccg caacttctac tgttggcgta
     3061 gtagaaagtc caaggtctac cacattaatt ccaagccctg tcagcgtatt cactaccagc
     3121 tcctgcacca tgctgccgct gatacggcca tcacggccta ttacaatttt tgtggccggt
     3181 ttatcttttg caatgattgt cccgaatgca gctgcaaatt tcactatatc caccggggta
     3241 aggttatccc cgggtttgcc gcctattgtt ccccttatcc ccgaaatgct tttaattaat
     3301 gccacatttt ttcttttagg gtggcaaaaa tacgtttttc gctttcaatg ccgtttttaa
     3361 ttgcgtcctt acatttgcat atgtccgaaa aagcatggta caaagactgg tttaattctc
     3421 ccttttatca taaactctat tttgaaagag atgaaaaaga agctgagaac ttcattcatc
     3481 agctggtaac ccatttaaaa cccctgccag gcagcaggat gcttgatgta gcctgtggta
     3541 aaggaagaca cagcaaaaca ctggctgccc ttgggtttac tgtgaccggt atagatatat
     3601 ctcccgacag cattgcctat gccaaacaat ttgaaaaaga aaatcttgat ttctttgtgc
     3661 atgatatgcg gcttcctttc tggggcaatt attttgactt tgcctttaat ttttttacca
     3721 gttttggcta cttcaaaaca agacgggaac atgatgatgc gattcgtacc attgccaaaa
     3781 gtctgaaacc gggtggcagt tttattatcg actacctcaa cgttcattac gcagaagaac
     3841 atttgcttca ccacgagaca aaaaaaattg gcagcacagc gtatgaaatt acccgctggg
     3901 atgacgagac acatttttat aaaaagatct ctgtaacaga tcctgctgta gaaggcagta
     3961 tcgaatacac tgaaaaagta gccaaattta acctgggtga ttttaccgat atgctcagct
     4021 tccagggcat gcaggtaaat gaagtatatg gcgattacct cttcaatccc tacgatgtaa
     4081 gaaaaacacc ccggatgatc attattgctg agaaaaaaag gaatgacaag gaaggcaaca
     4141 aaaaaagatt atatagcgac ggcagaagta cagatgccct gacctaatct ttctgattgt
     4201 tcatcaacaa gtatttcgat gtctcgaaaa aagcatgcgt aaagacggat aattctttcc
     4261 ggatggagtc aatttgaggt ttactgtatt tggttaatgc gctgctgtgc ataggcacca
     4321 gttcctcatc agggaagttg atattcttag tgaaataaaa atcatctgtc accataccta
     4381 taccaccggc tgtattggtg ataaaagcaa agttattttt cttatctgtg tcgagcaggt
     4441 cacggcccaa ggtggtattg acataagact gatgcaacaa ccctgcaatt gttggcaaca
     4501 catcgatctg agaaaccact tcttttcttc tttggggagt cagcagatca ggtgcataga
     4561 acaacaaagg aacatgctca tccgtgagac ggtgctctgt ccagacaggc ggatacattt
     4621 ctcccgcatt tcctgcaagg ccatggtcgc caacaaaaac aaagatggta ttatgaaaat
     4681 aggcttcctt ttgtgccgct tcgataaatt tccggaaaca ataatctgaa tagcgaaaac
     4741 aattgtattc gtccagcgat tcaaacccgt attttttaag ttcttcctcg ggaactgttt
     4801 ttcttacaaa ttcagtatca ctgtcaggaa tagtccggtt atatggccgg tgattacccg
     4861 atgtttgtat ataggcaaag aaaggtgagt tctttttctt aaaaacttca ttggcctcca
     4921 ggaacagatc cttgtcactg ataccccaca catttacttt tggttcctta aaattctcct
     4981 ccgtatgcat ctgcaatccc tttatattac tcagcaatcc gtcaaaattg ctgaactctg
     5041 cgctaccacc gaggaagtag tgtttttcat aatcctcaaa atggttaata attgttcgct
     5101 gtttaacagc atcgggatta cggctggaga atttgaataa ctgtgcatcc ggaattccgg
     5161 aaagtatggc aaacaaagcc cttgccgtag agaaatgagg tgcaaaacat ttttcaaaaa
     5221 atattccgtt tcggcacagg gagtcaaaga aaggtgttgt gttcaacggg ttgccgctca
     5281 tcgaactttt atacatgctg aacgattcgc attgtaccag cacaatattg gggcggcttt
     5341 caaatgaatt acttctggga gcaatctgcc ttcgataagt aaaattattt ttatctggca
     5401 actgcatcca atcagccata atcggaaaaa cctcccttgc tttagcttca ttatggtctg
     5461 gcttgcgcag tctcagtgtt gcaaagaagt tctgcaatgg attgatagca atgtaagact
     5521 tgaaactatt tttgaaacga aagctgtcat ttcttcccaa tggaggccag gaaagattgc
     5581 cccaggcaaa aaagaccagt acaaggatag ctatgaaaaa gaattttcgc cggtaaggaa
     5641 tccctttccc gtcagtacgg ttgatcacca tccagtggct gcgatgatac atccagcgaa
     5701 ataaaataac cgctaccacc aaacctgata acatcaggat catcgggtag gtttgccaca
     5761 tcattttcca tgaaatactg aaatcctctg caaaattcat tgcaccggca tccagcgggg
     5821 tctgattata ggagaaactg ccaaaccctg cagcaaaaaa gaaaaaaatg ataaaagtaa
     5881 ttatagccag gtaccatgtc caccatttct tattgcggga agagtaaaaa ggggacaaat
     5941 gcggatgcaa acttgtaatt acaatcggca ataatacaaa ggcaatccat ctcagatcgt
     6001 agcgggcacc catcaaaaaa gaaggaattg tttcagtgaa tgttattccg tcaggccgaa
     6061 aagcaaagaa ggtaatcaac ctgaaaaggg gaaaaatgaa aaaaaagatg acaaagaggt
     6121 ttgccaccca caataaggtt tttggtattt tatagcgtac taacatcccg gtaaaaaagc
     6181 ctgtaattca ggcggtcaaa tgactacaaa agaatagaat ttaaacggtt tcaacaacaa
     6241 gttgttaagt tactgccggc cgctatacgt attcgcaggt atcagttact tttgctgcca
     6301 tgtttccact gaatcttgtt ttttggccct ggttgcagcg attggatgaa agcctgttcg
     6361 taaccatcaa cagtcaatgg accaacccgg tttttgattc agtaatgcct tttctgagaa
     6421 aatcagatca ttgggtgccg ctataccttt tcctgcttgt ttttatattg atgaacttca
     6481 gaacaaaagc cgtatggtgg atcatattct tcattcttac ggtgggtatt acagattata
     6541 tcggcaccaa tgcgtttaaa tacgggtttg aaagaatccg gccctgcaac agtgccaata
     6601 tgctggaaca tttgcgtttg cttgttccct gtccttcagg ttttggcttt acctccaatc
     6661 atgcagccaa tcattttggc atggctactt ttctattcct tagtttccgt cacctgttta
     6721 agaactgggt atggctggct tacctgtggg ctggttccat agcttatgca caggtttatg
     6781 ttggggttca ctacccgatg gatatcattg ccggtgctgt ttggggatta ttggtgggaa
     6841 ctattacggg tactgttttc aacaaatggt ttggattaaa aggagatcat ttggagtatc
     6901 aatgagctat tttcttgtta tgaaaaagca tgtacctcgc tgtttcgtaa taacccagtg
     6961 ttaattgcct gatgagtgat ctttccttct ctgatggctc gtcgggaagc agcgggttgt
     7021 tatgcgtaag cggataaaaa ccttcgcctt ttgtctgcag gctgtattcg taataatatt
     7081 ttccgccaac taacccgata agtctcctgt caaaatccag tacaaaagca tgattgttga
     7141 tgtaatcaga atctgaagaa ggtggcagtg cgaaaatatt tctgcccata gtgctattgg
     7201 taaccggttt gttaattaat cctgcgatag taggcataat atctacctga gacacttttc
     7261 tttctattac ttgtggctga atttttcccg gtgcataaaa gagcatcggt acatgctgca
     7321 aggtcaggct ttgatctgtc cacactttgg gcatcatatc accggctatg ccccggatgc
     7381 cgtgatctcc cacaaaaata aaaatggtgt tattgaaata tttttcttta gcagccgcct
     7441 ccataaaaac tttataggta taatccatgt aggcatatgc attaagctcc tcgtttgtcc
     7501 agaaaccata tttatccagc gtatcttttg ggaaacttct cattttaata tagagccggt
     7561 cttctttggg gatagtgtac gggcggtgat tatcggnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn ncaaacatca attttaggtg atgaatagtc ctgttgttcg taaaggtgca
     7741 gcccttttat attgtccttt aatacgccgc ggatattagc ccaactggca ctgcctccga
     7801 ggaaataaaa tttttcataa tccttcagat cattaagaat agtatgctga tcaacaaggt
     7861 tagggtttcg gctggcggtt ttaaattctg tcacatccgg aataccggta ataatggccc
     7921 agatgccacg ggcagtacca taggcaggag aaaaacagtt cttgaaaaaa gcaccttgct
     7981 tacacaactc gttaaaataa ggtgttgtat tcagcgggtt tccccacatg gagcttttat
     8041 aggcgctgaa actttcacag atgaccaaaa ctacattcgg cttctcaatc gttggaacgg
     8101 aatcctgaac aggcattatc cttttgaaat tgagcgatgc agtatcccgg ttttttattc
     8161 caaaaacatc ggccatcaaa ttgtaatgac gcctcaccgc cttttcatca tacccaacat
     8221 tccggaattt gagggtactc ataaaactct nnnnnnnnnn gattcagcgc taaattggct
     8281 ttgtagtcac tgtccagttg gtaagcatca ctccagcgca agggaaattg gccgactctt
     8341 ccgaagatag ctatggcaaa aacaaaaaat agtactacat aacttaccgc atgattcagt
     8401 tttgaaatct tatcaggcaa ccccagaact gatttatata tccacctggt agcataaaac
     8461 attccggcaa atgcaatagc ccaccccaaa aggattttaa ttaccgggta actttgccac
     8521 accatggaag ctgatatacc ggcatcttcg acgtagctta aaacacttgc atttagccgt
     8581 tggcgtaaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaggtgtaa
     8641 acaacaataa ccagcaaacc aaataaccgt gtaaacacaa gcaaaatttt gcgggaaata
     8701 acctgggtaa atggcttgta gaaataaaaa atggacgata acagcagaac aatcatcagg
     8761 aagacacaga ccacccgggc atcataacga aatcccatcc acaaagcatc tcctatagcc
     8821 ttttcaccac ggggagtaaa tacaaaatga aaaatgatgc gaagaatggt gagcagtaag
     8881 agaaaaaaca aaccattaag cagcatccag cggaccagtc gggaatgtat gaacttcttc
     8941 atgtaaggca agctgttagg ttgctaaagt atcaaatatt gatggcatag tagctgataa
     9001 atgactggaa caaatgaggc ttaagattaa ttacaagttc ttctgacatt gtcgcagtgt
     9061 aatcgggctt ttttcttaaa aatgcagatt tcctatcttc gtttttaacc aactggtgtt
     9121 tcttaatgga cataatcata ctaggctttc ttatactgtt aaatggtctt ttttcaatgt
     9181 ctgaaatagc attggtatca gccaggaaag ggagactgga acaccaggca gaaaaagggg
     9241 ataaaaaagc caggctggct cttgaaatgt ccagccatcc tgaattattt ctttcagcag
     9301 tacaaatagg tatcacatta atctctattg ttacgggtgt ttattccggt gaacgtttca
     9361 gtaaagacct gcagcccatt gtggagaaaa ttgaaaccct caggccctat gccgaaggta
     9421 tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnctgatcc caaaacgtat cggtctttta cgttctgaaa aaatagctaa
     9541 ggcagtagca ggcccgatga agttctttgc acgcctcttt catccatttg tatggctgct
     9601 caataaaaca agtaatgttt ttttccgggt gttcaatatc aagcgcatca aggatgatgc
     9661 ggtaacggaa gaagagatca aaacgctgat aacagaagga accgaggccg gaactattga
     9721 tgaagctgaa caggaaatca tcgaaagggt atttcatctt ggcgaccgca atatcacttc
     9781 actgatgaca caccggagtg atattatctg gtttaatctt gacgactcag aagataagat
     9841 caaggaaaaa atattacagg aaccccattc ttcctatccc atctgcgatg gctccataga
     9901 taatatcaaa ggtattgtat cgatcaaaga cctgtatatc agtccggaca gtatgctttt
     9961 caaagacatc atggtaccgg ccttatttat cccggaaaac aactcacctt accaggtgat
    10021 ggaaaagttc aaacaatcaa agatccactc tgcttttatt gtggatgaat acggaagtat
    10081 tttgggattg cttaccctaa acgatatcct ggaagctatc gtaggagata taccccaacc
    10141 tgacgttcct gattatgaga tccgtaaaag agatgacggc acttacctgg tagacggaca
    10201 gattcctttt tacgacttcc tcacccgttt tgaaaaaaca gaatggatga atgaaggtga
    10261 acatgatttt gacacccttg ctggttttat ccttcacgaa ctggaacgca tccccaaaac
    10321 cggcgacaag attgactgga aaggtttcaa aatagaggtg atggatatgg acggacatcg
    10381 catcgacaaa ttactggtta cactcagtga agaattgaaa gaagaaatgg aagactgacc
    10441 ttcttagaaa tttactgtca cctcggtatc cgggtaactt agcctggtgc tctttcggtt
    10501 accacctaca aatacatgca tgatattaac ctggtcatca aaaaaatcat acatcaggtt
    10561 gttattgata gtaaaggcag aaacagcctg cacattttca acttccaggt aagcaaacac
    10621 agcttcttta tcatgttcaa agcccaggta agaaaatttt accggctttc cattcgcctt
    10681 tacagcaaga tgagaaatga tgtacttctt caccagtgta tccattgccg ccttattggg
    10741 tgggttaatt aaatcaactt ttgtgttata atttttggcc agcacccctt caaaatcgtc
    10801 tgtaaaaatt ttgcaagtca cctccagtgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aaaccccgca ttacagaatt
    10921 tgctaatttg acagaaggtt cttccttctt gattccgagt atcaaaaagg ctgataagag
    10981 cgagaaacta aaccatttat tgagtaatga agccattaaa acaattattt tcatttgtaa
    11041 ctttcaaggc cttattttca ggctttgtca tcagacgcta aaaatatggt tttggttcga
    11101 accgatcgat aaaattagta tgagcgactt cggattgtat tttggaattg gctgggatca
    11161 catcatgagc tgggatgccc ttgatcattt gctgtttgtt acagccctgg ccaccatcta
    11221 ttacctcaaa gactggaaac aggttttaat cctggtcact gcatttacca ttggtcattc
    11281 acttactttg gtgatgagtg tgctggacat tatccgtttt ccttccaact gggtggagtt
    11341 cctgataccc tgtaccattg tcatcacagc cttcagtaat ctttttcaaa aaaagtttac
    11401 cccgaagtca attcggatta attatttcct ggcattattc ttcggactga ttcatgggtt
    11461 gggctttgcc aatacgatcc ggttcatgct ggcatcggat caaaatatag gatggggctt
    11521 atttgccttt aatgtgggcc ttgaagcggg gcaattagtt gtggtagctg tttttttatt
    11581 ggcggcacaa gccatcacat ctgtattcaa tattgtgagg agagagtggg taattttttt
    11641 atcagcggcc gtattcagcc tgtctttgca aatggtattt caaagaattt cgtcattata
    11701 aacaaaaacg caaaaatgaa acgtttcacc tctgctattg cattgttatt gtgttttagt
    11761 ctagcgggtt ggtctcagaa tattcagaac aaccccagca gtaatcatgg taacaagttt
    11821 gaacaaatgg gaagcatctt ccctacccca aatgaatatc gtactgccag cggcgccccg
    11881 ggccccaaat actggcagca gcgttgtgat tacgacataa agtgtgaact tgatgaaaaa
    11941 aacctgaaac tcaccggtag cgaaacggtc acttacttca acaactctcc gaatgaatta
    12001 acctatctat ggttacaatt ggacgagaac cagcacagca gtacgaaaaa tgccaactac
    12061 cagaacagta acccgatgag caggcaaaca agcccccaga tgctggacag ggccgatgaa
    12121 gcaaaaacgg ataatggtta tggtttcaat atcacaaaac tgactgatgc agccggaaaa
    12181 gatctgaaat acaccatcaa caaaacaatg atgagggtgg aactgccagc cgtattaaag
    12241 gctggacaaa aattcacttt caaactggac tggaattata aaattgccga cagaatgggc
    12301 cgcggtggtc gtggcggtta cgaatacttt gctgaagatg gcaaccacct tttcaccatg
    12361 acacagtggt atccccgtct ttgtgtgtat agtgatttta agggatggca gaaccagcag
    12421 tttacaggtc gtggtgaatt tgcacttaca ttcggcaact tcaaagtaca aatgaccgtg
    12481 cctgctgacc atgtgatcat gtcaaccggc gaatgccaga attatgctgc agtattaaca
//
LOCUS       DAORLS010000055        12480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2691_length_12480_cov_2.011429, whole genome shotgun sequence.
ACCESSION   DAORLS010000055 DAORLS010000000
VERSION     DAORLS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12480
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2691_length_12480_cov_2.011429"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    163..259
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<260..981)
                     /locus_tag="PK977_06130"
     CDS             complement(<260..981)
                     /locus_tag="PK977_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17724.1"
                     /translation="MEVHHHSHTARKKWTHYFWEFLMLFLAVFCGFLAEYQLEHKIEK
                     DREKQYIASLYQDLKTDTLNLGNLLKGNMDDMANYDSLFNIFKTKKYLQETSGAYYFG
                     RRLVTLRPFYPTDGTITQLENSGGLRLIRKRAVVDSIQSYKLAISSLQSIQDFQREHF
                     ELARSVMGKIFDADVFDIMMGDGSNPLSRPDKNYPLMPFTEAQLNDFHIPMNFIRRNK
                     QTQLVLITRLQKKASDLMKLIE"
     gene            complement(990..>1359)
                     /locus_tag="PK977_06135"
     CDS             complement(990..>1359)
                     /locus_tag="PK977_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17725.1"
                     /translation="KSSGHLRLIRNKEVAGKVSSYYSSLSELDTYNDVGMIWSNNYAQ
                     AMSKLFDGAALLKIIKEKKEVPLTGDALISEDKIAINELLTSAGYFYGALSLNNTVAA
                     DRKNNAIQLIELMKEKYRLK"
     assembly_gap    1360..1551
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1737..2507)
                     /locus_tag="PK977_06140"
     CDS             complement(1737..2507)
                     /locus_tag="PK977_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17726.1"
                     /translation="MEVHHHSHTARNKWTHYFWEFLMLFLAVSLGFYAENTREKILHK
                     KEVKTQLNSMLSDLQSDISLFDSVTNRNSHGAQMADSLVELLHSDITNTTEIYFAART
                     VTANVGYYYSNSKSFDQLKAAGLLRYIKNKDLLDSIGTYYASFQWLANQIDLLLRKLD
                     EIHKGNTRLFDSYVFQQMVVSIKIIGASSVGGQRTAISKPMVKPSLLSTDIKDINTVS
                     LNYHYYSTTIKFYIRNAMALQNRAKGLVEKIKKEYHFD"
     gene            complement(2514..4043)
                     /locus_tag="PK977_06145"
     CDS             complement(2514..4043)
                     /locus_tag="PK977_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF17727.1"
                     /translation="MKRTLALTGFVLLITACSVAQNENSRAFIGDSLDQYVEKALGDW
                     QIPGAAVAVVKGSKIIYMKGYGVKEMDTKNSVDENTLFMIGSNTKAFTGTALAMLESE
                     KKLSLDDKVQKWLPDFKLYDPWVTKEANIADLLSHRLGFETFQGDFMYFDSDLTTAQV
                     REKFGKVKPMYSFRNKWGYCNAAFMTAGEIIPKVTGKSWADFVTERIFKPLGMSRSLA
                     LCKDIETATNKAGAHTVVMGELKKIPYGQLDNMAPAGSISSSVSDMSKWVLALLNDGK
                     MEEKQIIPSSAIKRTRQPESIMGDGGHMFNKAHFNLYGLGWFLEEYAGRKTVAHTGGV
                     NGFVTSVCLVPEEKLGIIVLTNTDANGFYEALRNELQDAMLGLPFRDYSNVYLSYHRS
                     SEEKNEKEWANLSDTIAMKKPTSLPLTAYTGEYNHDVYGKMTIKTEEGKLVMRFEHHK
                     GIFGTLEALGGNRFFCIYSDPLYGRKKLEFTVQAGKVKSLTVRVADFVEFTPYEFIKI
                     K"
     gene            complement(4140..4889)
                     /locus_tag="PK977_06150"
     CDS             complement(4140..4889)
                     /locus_tag="PK977_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17728.1"
                     /translation="MRPFLYILAILFLFCSSAAGQFYKIRGTVYDSSRNYPIELVSVL
                     STSGTGTVTNSEGYYEIEVQEKDSIWFSYLNKPTMKFPVLKIINPMAFDISIQINVPL
                     LREVKIKPRIYKQDSAQNRLDYAKIFNYQKPKLKTVTPQYGAGAGFDLNELINMFRTK
                     RNRSIASFQKRLLEEEQEKYINFRFNKALVRRLTLLDGNELDSFMRVFRPSYIFTTMA
                     GDYEFQYYIKESLYRFKRGLPPLDPFRKEEE"
     gene            complement(4991..6295)
                     /locus_tag="PK977_06155"
     CDS             complement(4991..6295)
                     /locus_tag="PK977_06155"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF17729.1"
                     /translation="MKKIILISSLLIFHVTSFAQQTVVAASKATVLTPAKPEAAGFSA
                     ERLQRIDENINQWMEDGRLNGGVALIIRNGKIVYHKAFGFDDPEKTKPIRTDNIYRIA
                     SQTKAVTSAAIMMLYEEGKFLLDDPVSRYIPEFAKQQVLDKFNPADTTYTTVPAKSEI
                     TIRQLLTHTSGISYPQIGTKEANAIYAKAHLMAGIGVDNSRSLAADVKTIAKLPLMHQ
                     PGEKFTYGFNTDILGYLVEYFSGMSLDVFFRKRIFEPLGMNDTYFYLPAAKHSRLVNL
                     YVEKDKQLEKPGAMVDFNGEINTDYPKMQNTYYSGGGGLSSTAKDYAVFLQMLLNGGT
                     YNGKRLLSRHTVRLMTMNQIGDVSRGANKFGLGFGITTEKGSSLLPTPEGVYEWGGAF
                     ATTYWADPKEKLIGIFYHQLWRSTFGETANKFKVLVYSALND"
     gene            complement(6327..>6795)
                     /locus_tag="PK977_06160"
     CDS             complement(6327..>6795)
                     /locus_tag="PK977_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRF17730.1"
                     /translation="NNPKAGVLEIARKEQIPVLIIEKERFLHGDAYLHELIEKRTDLI
                     VLAGFLWKIPPTMINAYRDHIINIHPALLPKYGGQGMYGQRVHQAVIDNKEKESGITI
                     HYVDEVYDNGKIIFRASCAVRENDTAVSLSQRVQALEHEHYPRVIETLINQFQ"
     assembly_gap    6796..6916
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6917..7219)
                     /locus_tag="PK977_06165"
     CDS             complement(<6917..7219)
                     /locus_tag="PK977_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17731.1"
                     /translation="MFEKLQKKWKVNGLQLALILCTFAIGGSATGFVGKKIMNVLQIQ
                     QDWLWAVVYILLITIIWPMAVLIVSIPFGQFRFFTNYIRKLGSKLGLVKAPAAPEVS"
     gene            7559..8842
                     /locus_tag="PK977_06170"
     CDS             7559..8842
                     /locus_tag="PK977_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF17732.1"
                     /translation="MIPSKPIGRRLIVFCFSFFVFSVVNNSYAAGDPAAGKAVFLGKC
                     AACHNVFKDGTGPALMGMEERGKWADHNELLKWINNPAAYMANDPYTQGLKAKYGSMM
                     TGFADVTLADVDNIVAYVNDEVAKKNKGGEKTDVPQEAPSGNNAIIFGIISLILAVIA
                     LILMQVNSNLKKMSDDAEGIIRPEPVPFYRNKAYIAVLALVFFVIGGYLVAKGAIGLG
                     RQKDYQPEQPIYYSHKVHAGINQISCLYCHGSAWDSKHAAIPSVNVCMNCHKAIQTYE
                     KGPKLYDESGNEINGTAEIQKLYAYAGFDPNNASKWDPSMIKKPIEWVKIHNLPDHVY
                     FNHSQHIRAGKVQCQSCHGEITAMDEVKQVSDLSMGWCVNCHRETKVDFNYTEGKGNK
                     FYSIYEKFHNDIKTGKMDSVTVNDIGGLECQKCHY"
     gene            8903..>11838
                     /locus_tag="PK977_06175"
     CDS             8903..>11838
                     /locus_tag="PK977_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRF17733.1"
                     /translation="MSQNKYWQSFGEVNDPENFKKLTQDEFKEELPFEGFDDKGLIDA
                     KAPRRDFLKYLGFSTAAATLAASCKVPVRKAIPYANKPENLVPGVAKYYATTYVQEGD
                     VVPVVAKVRDGRPIKIEGNVLEGSSYATGGTSARAQASVLDLYDMYRLKYPQHRVAGG
                     ADAEVKFEEVPTFEQFDKMVGDAMKAAGGTTVLLTSTVVSPSTKEIIAAFPNLKHVQY
                     DAVSYSGMLLANEASGFGRKIPSYDFGAAKVIVGIGADFLGTWLSPVEFAKGYAKGRR
                     IDEKNPVMSKHYHFEGHLSMTGSNADERFTHRPSETGAVVAALAAAVGVSGVSSSVTD
                     TKLKAGIEKAAADLKAANGEALVVCGSNDTEVQVLVNAINNAIGAYGKTIDWSKPVNY
                     RQGIDSEFAQLVADMEAGTVGTLMIHGANPAYTWHDADKFKAALKKVKVTVSFNEKMD
                     ETTSLCEYVLPNHNYLESWGDAEPKAGIIAFQQPTIYPLFKTRPYQTSLLRWSGGTQD
                     YEDYFKAYWVGKLGSEEAFNTALQNGLKEDAPVEASAGSYNGGAVAAAAAEIAGYKKA
                     TGTEVVVYQKVSMGTGAQSGNPWLQELPDPVSKATWDNYALISLPKAKELGIKVDMDY
                     EYYPEKPVIEITVGKNKVELPVLVIPGMNADTIAVAVGYGRADTLGVAAAGVGKNVYP
                     FATFNGKTVAYAATGATATNLKKKYKIAQTQKHNSYENRQEVIRETTLASFKKDPNVI
                     LSYSKHLQDAFYKNSNGSYRSNATLYPDPNTRQHEIHWGMSIDMNACNGCGACIVSCH
                     AENNVPVVGKREVLRYHDMHWLRIDRYFISDEKDPDNLKGVVFQPMMCQHCDNAPCEN
                     VCPVAATNHSQEGINQMTYNRCIGTRYCANNCPYKVRRFNWADYTGADSFPNNRDQQL
                     VGKLDSVIEQMNDDLTRMVLNPDVTVRSRGVIEKCSFCVQKIQAAKLDAKKENRVLGG
                     DDVKTA"
     assembly_gap    11839..11919
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11920..12152
                     /locus_tag="PK977_06180"
     CDS             <11920..12152
                     /locus_tag="PK977_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17734.1"
                     /translation="VREENKQRSYHVLEQLHVLPNVTYMAKIRNTEEIDAHWGGDHAA
                     ESHEEGGHGKEAHPADSTSKVPTGSTMEQGQH"
     gene            12219..>12480
                     /locus_tag="PK977_06185"
     CDS             12219..>12480
                     /locus_tag="PK977_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRF17735.1"
                     /translation="MALLRYESQVRPPLVEGEKDYHQVTEDICRPVEAKPSRLWWIGF
                     LISVALLIFGIISVTIEVIYGTGQWNLNKTVGWGWDITNFVWW"
BASE COUNT         3377 a         2612 c         2654 g         3346 t
ORIGIN      
        1 atagctgtat cggctttcag gtcttcgtat aatgtttttg aatattgctt ctcccggtcc
       61 ttttctattt tatgctccaa ttggtactcc gccaaaaacc cacagaatac agcgaggaac
      121 agcatgagaa actcccagaa gtaatgggtc catttttttc tgnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnt cttcaatcaa tttcattaaa tcagatgctt ttttttgcaa
      301 tcgtgtaatt aaaacgagtt gtgtttgttt atttcttctg atgaaattca ttgggatatg
      361 aaaatcgttt aactgagctt cagtaaaagg catcagtgga taatttttat caggcctgct
      421 aagcggattg ctgccatccc ccatcattat atcaaacaca tcggcatcaa aaattttacc
      481 cataacactt ctggccagct caaaatgctc cctttgaaaa tcctgtatgc tttgtaaact
      541 tgaaatggcc agcttatagg actggatact atctacaaca gccctttttc ttataagccg
      601 taagccacct gagttttcca gttgagtaat agtgccgtca gtaggataga aaggccggag
      661 ggttactaat cttctgccga agtaataggc gccggatgtt tcttgcaaat attttttggt
      721 tttaaaaata ttgaataacg aatcataatt ggccatatcg tccatattac ctttcaataa
      781 attgccaaga tttaaagtat cggtttttaa atcctggtac agagaggcta tatattgttt
      841 ctccctgtct ttctcaattt tatgttccaa ctggtattct gctaaaaacc cgcagaacac
      901 agcgaggaac aacatcaaaa actcccagaa ataatgagtc catttttttc tggcggtgtg
      961 tgagtggtga tgtacttcca taataggtat tattttaaac gatacttttc cttcattaac
     1021 tcaattaact ggattgcatt attttttctg tcggctgcta cagtattatt tagcgacaag
     1081 gcaccataaa aatatccggc acttgtcagc agttcgttaa ttgcaatttt gtcttcactg
     1141 attaatgcat cacccgtcaa cggaacttct tttttttctt ttattatttt gagtaaggct
     1201 gcgccatcaa acagttttga cattgcctgc gcatagttgt ttgaccatat catccctaca
     1261 tcattatagg tatccagttc agacaacgaa ctgtaatagg aagaaacttt tcctgctact
     1321 tctttatttc gtatcaatcg taagtggccg gagcttttcn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttcaaatca
     1561 tccagcatgt tatgcatgta agttttctct ctgtcttttt caatcttatg ctccaactga
     1621 tactctgcca aaaaaccgca gaacaccgca aggaataaca tcaaaaactc ccagaaataa
     1681 tgggtccatt tttttcgtga ggtatgagag tggtgatgta cttccataat ttatatttaa
     1741 tcaaagtggt actctttttt tattttttca acaagccctt ttgcccggtt ttgaagcgcc
     1801 atcgcattac ggatataaaa cttaatggta gttgagtaat agtgatagtt cagcgataca
     1861 gtatttatgt cttttatatc agttgaaagg agggatggct ttaccatagg cttgctgatt
     1921 gctgttcgct gaccacctac agaactggcg ccaataattt ttatactcac caccatttgt
     1981 tgaaaaacat aactgtcaaa tagccgggta tttcccttgt gaatttcatc taacttccga
     2041 agaagcagat ctatctgatt ggccagccat tgaaaggatg cataataagt gccgatacta
     2101 tccagcaaat ctttattctt tatataccgg agtaaccccg cagctttcaa ctggtcaaat
     2161 gattttgagt tggaataata atagcccaca ttggcggtaa cagtacgggc agcaaaataa
     2221 atctctgttg tattagtaat atccgagtgc agcaattcca ccaacgaatc agccatctgc
     2281 gcaccatgac tattgcggtt tgttacggaa tcaaaaaggg agatatctga ctgcaggtcg
     2341 gataacatgg agttcaactg tgttttcact tcttttttat gaagaatttt ttcccgggtg
     2401 ttttctgcat aaaaccccag tgaaacggcg aggaataaca ttaaaaactc ccagaaataa
     2461 tgagtccatt tatttctggc ggtatgtgag tggtggtgta cttccataaa tcattatttt
     2521 atttttataa actcgtatgg agtaaactcc acaaagtctg ccacccttac agtaagtgat
     2581 tttacctttc ctgcctgaac tgtaaactcc agtttttttc ttccatatag gggatcgctg
     2641 taaatacaga agaaacggtt gcctcctaat gcctccagtg ttccaaagat gcctttgtgg
     2701 tgttcgaaac gcattaccag ttttccttct tctgttttaa tggtcatctt tccatataca
     2761 tcatgattgt attctcctgt ataagccgta agcggcagcg atgttggctt cttcatggca
     2821 atggtatcac ttaaattggc ccattctttt tcattttttt cttcactgct acggtggtag
     2881 ctcaggtata cattgctgta gtcacggaaa ggcaatccaa gcatggcatc ctgcaattca
     2941 ttacgcagcg cttcataaaa accattggca tcagtattgg tgagtacaat aatgccgagc
     3001 ttttcttccg gaaccaggca aacactggtt acaaaaccat tgactcctcc ggtatgagcc
     3061 actgtttttc ttccggcata ttcttccaga aaccagccaa gaccatagag gttgaaatga
     3121 gctttgttga acatatgtcc gccatcgccc ataatggatt cgggctgccg ggtacgtttg
     3181 atagcagatg aaggaattat ttgcttttct tccattttgc catcgttcag cagtgccagc
     3241 acccatttac tcatatcact tacactcgag gagatactgc cggccggcgc catgttgtcc
     3301 agctggccat aaggtatttt ttttagttca cccatcacca cggtgtgtgc accggcttta
     3361 ttggtggctg tctcaatgtc tttacataga gcaaggctgc ggctcatgcc caggggctta
     3421 aatattcgtt ctgttacaaa atcagcccat gacttacccg ttactttggg aatgatttct
     3481 ccggccgtca taaatgcagc attacaataa ccccatttat tcctgaaact atacatcggt
     3541 tttactttgc caaacttttc cctcacttgt gcggtggtca ggtcagaatc gaagtacata
     3601 aaatctccct ggaaggtttc aaaacccagg cggtggctaa gcaagtcggc tatattggct
     3661 tctttggtaa cccatggatc gtatagttta aaatcgggca gccatttctg cactttatca
     3721 tccagtgata atttcttttc gctttccagc atagccagtg cagttccggt gaaggctttg
     3781 gtattgctgc cgatcatgaa cagcgtattt tcatctactg agttttttgt atccatttct
     3841 tttactccat accccttcat ataaataatt ttacttcctt ttacaacggc tacagcagca
     3901 ccgggtatct gccagtcacc cagtgctttt tcaacatatt gatcaaggct gtcgcctata
     3961 aaagccctgg agttttcatt ctgtgcaacg gaacatgcag ttatcagtaa tacaaaaccg
     4021 gttaaggcca atgtcctttt catttaaatt agtttaaggg tgaataaagg gaaagttagc
     4081 ttcagagggg aaactaaaaa tacttttctt ttttgatttg atgaagccgt cgcttatggt
     4141 tattcttctt ccttacggaa agggtcaagc ggaggcaagc ctcttttgaa gcgatagagc
     4201 gattctttga tgtaatactg gaattcataa tcgccggcca tagtggtaaa gatgtaagaa
     4261 ggacgaaaaa cccgcatgaa actgtccagt tcgtttccat ccagcagtgt cagccggcgc
     4321 accagtgctt tgttgaaacg gaagttgata tatttttcct gttcctcttc cagcaacctt
     4381 ttctggaaag aggcgatact cctgtttcgc ttggtccgaa acatattgat gagttcattc
     4441 aggtcaaaac ctgcacctgc cccatactgc ggtgttacgg ttttcaattt cggtttctgg
     4501 taattgaaaa ttttagcgta gtcaagtctg ttttgcgcag aatcctgctt gtatattctc
     4561 ggcttgatct tcacttctct cagcaatggt acatttatct gtatggaaat gtcaaaggcc
     4621 atcgggttga tgatcttcag taccgggaat ttcatggtag gcttgttcag gtagctgaac
     4681 cagatggaat ccttttcctg cacttctatt tcataatagc cttcggagtt ggtcactgtt
     4741 cctgtacctg acgtactcag cacactcacc agttcgatgg gataattgcg ggaactgtca
     4801 tacacagttc ccctgatttt gtaaaactgc ccggcagcag atgagcaaaa cagaaacaaa
     4861 atggcgagta tatataaaaa gggtctcaac agtgtttagg gttgcgggtt atcctgttaa
     4921 ctgatctacg gttcaaccaa taaggcggca agtaacaaaa gaaagtgcta aatcaatgat
     4981 aaaacaggaa tcagtcattt aaagcggaat acaccagcac tttaaactta ttggctgttt
     5041 cgccgaaggt ggatctccat aactggtggt aaaatatgcc aatcagtttt tccttcggat
     5101 cagcccagta agtggtggca aaagcgccgc cccattcata tacaccttcc ggcgtaggta
     5161 ataaggaact gcccttttca gtagtgatac caaaacccag cccgaactta ttggcccccc
     5221 tgctgacgtc tccgatctgg ttcatcgtca tcaaccgcac tgtatgtcgt gacagtaacc
     5281 gcttcccatt gtaagtaccg ccattcagca gcatttgcag aaaaacagca tagtcctttg
     5341 ctgtagaaga tagaccgccg ccgccggagt aataggtgtt ttgcatttta ggataatctg
     5401 tattgatctc gccgttgaaa tcaaccatgg ctcccggctt ttcgagttgc ttatcttttt
     5461 caacatacag gttcaccagc cggctatgtt tggcagcagg caggtaaaaa taagtgtcgt
     5521 tcataccgag cggttcaaaa attctttttc ggaaaaaaac atccaggctc ataccggaaa
     5581 aatattctac caggtagccc agtatatcag tattaaaacc ataagtgaat ttttcacccg
     5641 gctggtgcat gagcggcagt ttggcgatgg tttttacatc ggcggcaaga ctgcggctgt
     5701 tgtctacacc aattcctgcc attaaatggg ctttcgcata aatggcattg gcttctttgg
     5761 tgcctatctg cgggtagctg ataccggaag tatgggttag caactgacga atcgtaattt
     5821 ctgatttggc aggtacggtg gtataagtag tatcagccgg gttgaatttg tccagcacct
     5881 gctgtttggc aaattcagga atgtagcggc taacgggatc atccaataaa aactttcctt
     5941 cttcatataa catcataatg gctgcactgg taacagcctt tgtctgcgaa gcgatacggt
     6001 aaatattatc tgtgcggatg ggtttggttt tttcagggtc atcaaatcca aacgctttat
     6061 gataaacgat ttttccgttt ctgatgatca gcgctacacc tccgttaagc ctgccgtctt
     6121 ccatccattg gttgatgttc tcatcaatac gctgaagcct ttcggcagaa aatccggctg
     6181 cttccggttt tgccggggtg agcaccgttg ctttggatgc ggcaacaaca gtttgttgtg
     6241 caaaactggt tacatgaaaa ataaggagag atgaaataag aatgatcttt ttcatggtgg
     6301 ctgatttaaa aagaaaaaga aagatactat tgaaattgat tgatcagcgt ttcaattacc
     6361 cggggataat gctcatgctc cagtgcctgc actcgttgcg atagcgaaac agccgtgtca
     6421 ttttcccgga cagcacaact ggcccggaaa attatcttcc cgttatcata tacctcatct
     6481 acatagtgaa tagtgatgcc agattctttc tccttgttat cgataacagc ctggtgaacc
     6541 ctttgcccgt acattccctg tcctccgtac ttgggcaaca aggccgggtg tatattaatg
     6601 atatgatccc ggtaagcatt gatcattgtt ggcggaattt tccagagaaa acctgccagc
     6661 acaatgagat cagttctttt ttcaatcagt tcatgcaggt aagcatcgcc atggaggaag
     6721 cgttcttttt caattattaa taccggaatc tgttctttgc gggcaatctc taatacgccg
     6781 gcctttgggt tattgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnggat acctccggag cggcaggagc ctttaccagt cccagtttgc
     6961 tgcccagttt gcggatataa ttagtaaaga agcgaaactg cccgaaagga atactcacta
     7021 tgagtacagc catcggccat atgatcgtaa taagcagtat ataaacaaca gcccagagcc
     7081 agtcctgctg tatttgcagc acattcatga tctttttgcc cacaaaaccg gtggcgctgc
     7141 cgccgatagc gaatgtgcag aggataaggg ccagttgaag gccatttact ttccattttt
     7201 tctggagctt ctcaaacata tttacccgat aaaatcgtaa aaaagacccg tgccggttgg
     7261 tgagccgcaa aattgaggaa aaaacagcca gcatacagga gccgctccgt cattcttttg
     7321 ttttttatgt gcttgatctg tctcaaattt tcacatatgt tgaagcctgg cgggaagttg
     7381 accgctttgt gacaaacaac tttaggcacc ttacaagaaa ccccttaaat cggctgaaat
     7441 gcaggctggt agcggatcaa aaaaaagtta aaccagatga acttttatag tgatcctgac
     7501 ttacttttgc ggctgtttat cggtttttat ccacattttt tcagaatcaa ttgtgtctat
     7561 gattcctagt aaaccaatcg gccggcgact gatagtattc tgcttttctt tttttgtttt
     7621 ttctgtagtc aataatagtt atgctgcagg cgaccctgca gcaggtaaag ccgttttttt
     7681 aggaaaatgt gctgcctgtc ataacgtatt taaagacgga acaggacctg ccctgatggg
     7741 tatggaagaa agaggtaaat gggcagacca caatgagttg ctgaaatgga tcaacaaccc
     7801 ggctgcttac atggccaacg acccttatac ccagggcctg aaagccaaat acggatcaat
     7861 gatgaccggc ttcgccgatg taaccctcgc cgacgtagat aatattgttg cctatgtaaa
     7921 tgatgaggtg gccaagaaga ataaaggtgg cgaaaaaaca gatgttccgc aggaagcgcc
     7981 ttctggcaac aatgccatca ttttcggaat catctcgctg atcctcgctg ttattgccct
     8041 gatcttaatg caggtgaaca gcaacctgaa gaaaatgagc gatgatgcag aaggtatcat
     8101 ccgccccgaa ccggttcctt tctatcgcaa taaagcctat atcgctgtac tggccctggt
     8161 gttctttgtt atcggtggat acctggtagc caaaggcgct atcggactgg gccgccagaa
     8221 agattaccag cctgagcagc ctatttatta ctctcataaa gtgcatgcag gtatcaacca
     8281 gatcagttgt ttgtactgcc atggttcagc ctgggacagc aagcatgccg ctattccttc
     8341 agtgaatgtg tgtatgaact gtcacaaggc cattcagact tacgaaaaag gtcccaaact
     8401 ctacgatgaa agcggtaacg agatcaatgg tactgctgaa atccagaaac tatatgccta
     8461 tgcaggattt gatcccaaca atgcttctaa atgggatcct tccatgatca aaaagcccat
     8521 cgaatgggta aagattcaca acctgcctga ccatgtttat ttcaaccatt ctcagcatat
     8581 aagagccggt aaggtgcagt gccagagctg ccacggagaa atcacagcca tggacgaagt
     8641 aaagcaggtt tcagacctga gcatgggctg gtgtgtaaac tgtcaccgcg aaaccaaggt
     8701 tgacttcaat tacaccgaag gcaagggcaa caaattctac agcatttacg agaagtttca
     8761 taatgacata aaaaccggta aaatggacag tgtaactgta aatgatatcg gtgggttgga
     8821 atgtcagaaa tgtcactatt aatctgtaga gaaagaagaa gaccgctatt cagcacatta
     8881 tagaattatc aaattgatat acatgagcca aaacaagtac tggcaaagct tcggagaagt
     8941 gaatgatccg gaaaatttca aaaaactcac tcaggacgag ttcaaagaag aacttccgtt
     9001 tgaaggtttt gatgacaaag gactgataga tgccaaagct ccccgcagag atttcttaaa
     9061 atacctgggc ttcagcacag ctgctgctac attagcggca agttgtaaag tgccggttcg
     9121 caaggcaatt ccttatgcca acaaaccgga aaacctcgtt cctggtgtgg ctaaatacta
     9181 tgccaccacg tatgtgcagg aaggagatgt ggtgccggtg gtagctaaag taagagacgg
     9241 ccgcccgatc aaaattgaag gtaatgtact ggaaggaagc agctatgcta ccggtggtac
     9301 ttctgcccgg gcacaggcct ctgttttgga cctttatgac atgtaccgcc tgaaataccc
     9361 gcagcacagg gtagccggtg gtgcagatgc tgaagtgaag tttgaagaag tgcctacgtt
     9421 tgagcaattt gataaaatgg tgggagatgc gatgaaagca gcaggcggaa ccaccgtatt
     9481 actgacttct actgttgttt ctccttctac aaaagaaatt attgctgcct tcccgaacct
     9541 gaagcatgta cagtacgacg cagtttctta cagcggtatg ctgctggcca acgaagcttc
     9601 agggtttggc agaaaaatac cttcctatga ttttggtgca gccaaagtaa tcgtaggcat
     9661 cggcgctgat tttctgggca catggctcag cccggtagaa tttgccaaag gatatgcaaa
     9721 aggccgtcgc attgatgaga agaacccggt aatgagcaag cattatcatt ttgaagggca
     9781 tctcagcatg acaggttcca atgcagatga acgctttacg caccgtcctt ccgaaacagg
     9841 tgctgtagtc gctgcactgg ctgctgcagt aggcgtaagc ggtgttagct cctctgttac
     9901 agataccaaa ctgaaagccg gtatcgaaaa agcagcggct gatctcaaag ctgctaacgg
     9961 tgaagcactg gtagtatgtg gcagtaacga tacagaagta caggtactgg ttaacgccat
    10021 caacaatgct atcggggcct atggcaaaac aattgactgg agcaagccgg tgaattaccg
    10081 ccagggtatc gatagcgaat tcgcacaact ggtagcagat atggaagccg gtacagtggg
    10141 tacactgatg atccacggtg ctaacccggc gtatacatgg catgatgcag ataaatttaa
    10201 agcagcactg aagaaagtga aagtgacagt ttctttcaat gaaaaaatgg atgaaaccac
    10261 ttcgctttgc gaatatgtgc ttcccaatca taattacctc gaaagctggg gagatgctga
    10321 acccaaagcc ggtatcattg ccttccagca gcctaccatc tatcccctgt tcaaaacccg
    10381 cccttaccag acctctttgc tgagatggag cggcggcaca caggattatg aagattattt
    10441 caaagcatat tgggtaggca aactcggatc cgaggaagcc tttaacacag ccttgcaaaa
    10501 cggcctaaaa gaggatgcac cggtagaagc ttctgcgggt tcctataatg gtggcgctgt
    10561 agccgccgct gctgctgaaa ttgccggtta caaaaaagca acgggcacag aagtagtggt
    10621 ttaccagaaa gtttcaatgg gtacaggtgc tcaatccggc aacccctggt tgcaggaatt
    10681 gcccgacccg gtatcaaaag ctacctggga caactatgca ctgatttctt tgccaaaggc
    10741 gaaggaactg ggcattaaag tagacatgga ttatgaatac tatcccgaaa aaccggtgat
    10801 agaaattacc gttggaaaaa ataaagtgga actgcctgta ctggtaattc ccggtatgaa
    10861 tgcagatacc attgctgtgg cagtgggtta cggaagggca gatacattag gtgtggctgc
    10921 tgcaggagta ggtaaaaacg tttatccgtt cgctacgttc aatggtaaaa cagttgctta
    10981 tgcagcaaca ggcgctacag ccaccaacct gaagaagaaa tacaagattg cccagacaca
    11041 aaagcataac tcttacgaaa accgccagga agtaataagg gaaacaacgc tggcttcttt
    11101 caagaaagat ccgaatgtga ttctttctta cagcaagcac ctgcaggatg ctttttataa
    11161 gaattcaaac ggcagctatc gcagcaatgc cacactctat cccgatccga atacaagaca
    11221 gcatgagata cactggggca tgagcattga tatgaatgcc tgcaatggtt gcggcgcttg
    11281 tattgtttcc tgccatgcag agaacaacgt tccggtggta ggtaaaaggg aagtattgcg
    11341 ttaccacgat atgcactggc tgcgtatcga ccgctacttc atctctgatg aaaaagatcc
    11401 ggataacctc aaaggcgtgg tattccagcc gatgatgtgc cagcattgcg acaatgctcc
    11461 ttgcgagaac gtttgcccgg tagcagctac caaccatagc caggaaggta tcaaccagat
    11521 gacgtataac cgttgtattg gtacaaggta ttgtgccaac aactgtccgt ataaagttcg
    11581 tcgtttcaat tgggccgatt atactggtgc cgacagcttc cctaataacc gtgaccagca
    11641 actggtaggt aaactggata gtgtgatcga gcagatgaat gatgacctta caagaatggt
    11701 attgaatccg gatgtaaccg ttcgttcaag aggtgtgatc gagaaatgtt ccttctgcgt
    11761 acagaagatt caggcagcca agctggatgc gaagaaagaa aacagggtgc tgggcggcga
    11821 tgatgtaaaa acagcctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agtgagagaa gagaacaagc
    11941 agcgcagcta tcatgtactc gaacaattgc atgtgcttcc caacgtaaca tacatggcta
    12001 agatccgcaa cacagaggaa atcgatgcac attggggcgg tgatcatgca gcagaaagcc
    12061 atgaggaagg cggacatgga aaagaagcac acccggccga cagcacttcc aaagtgccga
    12121 ccggttcaac aatggaacag ggacaacatt gataaaggaa aattgactaa ctataaaaag
    12181 ctaacagctt agggctaata gctaaaagct caaataatat ggcattactc agatacgaat
    12241 cacaggtaag accgccactg gtagaaggag aaaaagatta tcaccaggta acggaagaca
    12301 tttgccgccc ggtagaagcc aaaccttcaa ggttatggtg gattggtttt ctgatatcag
    12361 tagcgttact cattttcggt attatatccg ttactattga ggtgatatac ggtaccggcc
    12421 agtggaacct caacaaaaca gtaggttggg gatgggatat caccaacttc gtatggtggg
//
LOCUS       DAORLS010000056        12383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2729_length_12383_cov_2.090120, whole genome shotgun sequence.
ACCESSION   DAORLS010000056 DAORLS010000000
VERSION     DAORLS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2729_length_12383_cov_2.090120"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PK977_06190"
     CDS             complement(<1..1010)
                     /locus_tag="PK977_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRF17736.1"
                     /translation="MRPVIKRFENLRITGYIQPQFQKAQSDGAPSYTGGNFSQYSSSR
                     FMLRRARIKIDYILPSKLRYPKALFTFQIDATERGVIVRDMFLKLFEIKRNLFSLTAG
                     LFARPFGYEVNLSSAFRETPERGRMSQILMPSERDIGIMVSHEPQEKGKSRNRFKFDI
                     GFFNGQGLSGTTDFDSYKDLISRVFIRPFSPGKWELSGGLSFLRGGWKNGTKYVYESG
                     VAANGDKVFVVDSSERNLGKSSPRHYYGGDIQVKLQHGWGESEWRAEYWFGTQPGTSL
                     ATSNPGTLPTSGGNPVPTYVRHFDGIFLLFLQNIISNKHQLMVKYDWYDPNIKVKKSE
                     IG"
     assembly_gap    1036..1060
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1332..1982
                     /locus_tag="PK977_06195"
     CDS             1332..1982
                     /locus_tag="PK977_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRF17737.1"
                     /translation="MAGLNSFLKIFMPKNKIFYELFEKVANNVATMGSILRDLVAEPD
                     FDKRAALIAQLEDLEHANDEHTHSLFTELGRNFITPFDREDIHYLATSLDDIADYIYA
                     AGKKINFYRVNPNDTGMQKFAELIEQGAEQVKIAVTELRDMKNMRSITEALVKINSIE
                     NQADDIFDFSIEKLFATEDDAKEVIKKREIYQVMEIVTDKCEDASNVIESIIIKYA"
     gene            2013..3221
                     /locus_tag="PK977_06200"
     CDS             2013..3221
                     /locus_tag="PK977_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRF17738.1"
                     /translation="MTPFLITIIALALIFDYINGFHDAANSIATIVSTKVLTPFQAVL
                     WAAVFNSVAFFIFKDHGVADTVAKTVKPDHIEGNMMVVIFSGLIAAITWNLLTWWLGI
                     PSSSSHTLIGGFAGAAIAAGGFGAVNSPIIIKTASFIFLAPIIGMIMAFIMSLWFLSS
                     FKKGWTSKIFSIAIIVLVGVFLSTNLEFDRTILAKKTDYESYIAQVIFYSKNFKWILL
                     AFILLVMAVFALYLSTLNAHKSNQWFKRLQLISSAAFSIGHGGNDAQKVMGIITAALI
                     AGGTITDFHQMPDWVPIACYAAIGLGTMSGGWKIVKTMGTKITKVTPFEGVAAETAGA
                     VTLFVTEALKIPVSTTHTITGAIMGVGATKRLSAVRWGVTFNLIWAWILTIPISALLA
                     AGVYSIVSLF"
     gene            3273..4328
                     /gene="galE"
                     /locus_tag="PK977_06205"
     CDS             3273..4328
                     /gene="galE"
                     /locus_tag="PK977_06205"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRF17739.1"
                     /translation="MAKILVTGGCGYIGSHTIVDLVQNGYDVISVDNNSRSNPVILKG
                     VEQITGKKIKNYKVDLCNFDDTFAIFQENNDISGIIHFAAYKAVGESVENPLLYYENN
                     LVSLINLLKCVQEFRIPHFVFSSSCTVYGNPDEVPVTESTPLKPAESPYGATKQMGEQ
                     IVQDFAKNGTTSCVLLRYFNPAGAHPTAAIGEMPLGKPQNLVPAITQTAIGRIGKMYV
                     HGNDYPTRDGSCIRDYIHICDLANAHTLSLKHIEEGKNKSSCEVFNLGTGNGISVMEA
                     IHAFEKVSGVKLNYETGPRRPGDVVAVYANNDLAKNVLGWNPVFSLDDMMSTAWKWEL
                     RLKADETVFTSQPGELN"
     gene            4402..5367
                     /locus_tag="PK977_06210"
     CDS             4402..5367
                     /locus_tag="PK977_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRF17740.1"
                     /translation="MLLKDIQSTGSKDTRSGFGDGIVEAARKNENIVALTADLAGSLK
                     LNQFMKEFPHRFFQCGIAEANMMGIAAGLTIGGKIPYTTTFANFSTGRVYDQIRQSIA
                     YSGKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVVVPCDYAQTKAATIAIA
                     DYEGPVYLRFGRPSWPIFTDGKLFEIGKAQFFSEGTDVTIFACGHLVWNAIQAGAILQ
                     EKGISVEVINIHTVKPLDEEAVINSLRKTKCAVTAEEHNIIGGLGDAIAQCATRNFPV
                     PIEFVGTKDTFGESGTPAQLLKKYGLDIPDIVAAAEKVMARKNNG"
     gene            5495..6049
                     /locus_tag="PK977_06215"
     CDS             5495..6049
                     /locus_tag="PK977_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF17741.1"
                     /translation="MPLTTTSDSELLVEFRNPVTKEKAYTQIIRKYQEKLYWHIRRMV
                     IEHEDANDVLQNVLIRVWNGLENFREDSQLYTWLYRIATNECLTYLEQQKKRTAVSLS
                     DVETGLSNKIKADRHFDANKLEWKLQLAMQQLPEKQRIVFNLRYYDEMPYEEMSRVLE
                     TSEGALKASYHHAVKKIEDYMLNH"
     gene            6085..6855
                     /locus_tag="PK977_06220"
     CDS             6085..6855
                     /locus_tag="PK977_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17742.1"
                     /translation="MTQRDNILQELKELDSRLANVASQNVYTVPRGYFDSLTDEVMSR
                     IRALEANNPADELNALSPQLGKLSKEMPYSLPKDYFETLTDNVLKTIQTGGLSSKDEL
                     AAISPLLSSLKKETPYSVPEGYFESLSPTRREEEVQPSAKVVSLFSRTWFRYAAAAVV
                     AGIIILASISLFTSEKEPGGKALAKFTRDVKKMDEQQKDDMIDFFDAGLDGKETAQVN
                     TDAKNSQEVKELLEGVSEEELKDFQEQTEAIEEVLMTN"
     gene            6862..>7227
                     /locus_tag="PK977_06225"
     CDS             6862..>7227
                     /locus_tag="PK977_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17743.1"
                     /translation="MKKWLIILFLIPGVATMAQNDEPTPQEGKIQERMREYIQNKLNL
                     NRNEADRFTPVFVRYFREFAQTHRQNRGDRLVLQQKIVELRLRYRGEFRQILDEQRAN
                     KVYLYEDEFRRKAVDIIREH"
     assembly_gap    7228..7278
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7373..8113)
                     /locus_tag="PK977_06230"
     CDS             complement(7373..8113)
                     /locus_tag="PK977_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF17744.1"
                     /translation="MKIIPLSEGTFTIDKTKHFVPFDENEHSLKDRPSGSLLVEIQPF
                     VVITSKDIILLDTGLGFERNGILQLHQNLMNVGINPSEVTKVLMSHLHKDHAGAVGIE
                     RDGLGARLSLPNAKYYVQRTELVYAFEKGFPSFIQEELECLRNNEQAVLLEGSGIIDE
                     YIRYEVSGAHSPHHQVFWVKEAGEIIFFGGDEAPQLQQMKHRFVAKYDYNGKKAMELR
                     QKWWEQGAKEKWTFLFYHDVKHPLWTFQ"
     gene            complement(8177..9517)
                     /gene="rodA"
                     /locus_tag="PK977_06235"
     CDS             complement(8177..9517)
                     /gene="rodA"
                     /locus_tag="PK977_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRF17745.1"
                     /translation="MNQRNPAISKGIDWSLIGIYLLLVVIGLTAIFAVTYKEGDPIIQ
                     SFFRFKTDYSKQFYFFCLGTLIGFFILLTDSKFFAATANLGYALGIFMLLLVFPFHSP
                     IKGTESIIKLGAFNFQPAELCKIFVALALAKYLSRPETDFTKTRAQLIAAGIALFPAA
                     LTLLQSETGLALVYFSFFIVMYREGLPSVILLIGIAVTALVVATIVIEPDTLAIALTV
                     LAALLIYIFRRQIKRRRQILTTIIFLWVLCVGLQRFAVPYAFKNVLQKHQVERIYDLF
                     GKDNPYLDKSAELTAEELKKKKESGTSYNVRQSKIAIGSGRIFGKGILNATQTRYDFV
                     PEQRTDFIFCTVGEGFGFAGSFVLLGLYLILLFRIVTIAERQRSTFSRSYAYGVAAVF
                     FFHIAINIAMTVGLAPVIGIPLPFVSYGGTSLLTFTILLFILIRLDADRQMVLR"
     gene            complement(9514..11607)
                     /gene="mrdA"
                     /locus_tag="PK977_06240"
     CDS             complement(9514..11607)
                     /gene="mrdA"
                     /locus_tag="PK977_06240"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRF17746.1"
                     /translation="MSVFNQSRSRIIRLIFGVTFLVIIGQLFYLQVMSGKFRQLAMDN
                     AVYPKIIYPERGIIYDREGKAILNNTISYDLMVTPAEIKGIDVQEFCRLMNLDTAEFN
                     KRILEAKIKNTSVRPSIFDDQLTPDIQARFEENSWKFPGFTLVERPVRTYPFNAAAHI
                     LGYVREADPKDIEKSGNFYRQGDYIGKTGLEAYYEKVLMGQRGVEYWIKDNKNRLVGH
                     YEKGSFDTAAVAGRGLRTYLDADLQQLAEKLMTNKVGGLVAIDPKTGGILAMVSGPNY
                     NPNDLTGPEGSKNFGRLQLDVAGPMLNRAINGLYEPGSTFKPLGALVALDEGLITPAY
                     GYPCGGRYMGCGSGKPACTHSGGGHSANLRNAIAYSCNSYFTHLYRMAADNPQYGGVK
                     KGYMKWREYMNLFGLGVRLGVDLPSENKGNIPDTASYNKQYRGSWNSCTNLTLGIGQD
                     QMQATPLQLANAMCIIANKGYYYTPHFVQKIDDETDDDTVYMNKFRVKHEVLTHIPDT
                     AYRAVIEGMNDVVKFGTARIAQIPGIEVCAKTGTAENYTILDGRRIKLPNNSMFVCFA
                     PKDNPKIAIAVCVQNAGYGATWGGPISRILIEKYLNDTIQKKSEADVDRISKANLIPA
                     HYKRLQYKADSSRAWEWFKMTKDSSYIKKFRRAQADTPKPPAPKPADKQKTITYIWMQ
                     EEKKVASALKSRVLS"
     gene            complement(11756..>12153)
                     /locus_tag="PK977_06245"
     CDS             complement(11756..>12153)
                     /locus_tag="PK977_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRF17747.1"
                     /translation="TISRFGLLVIGFVTGLTLDYFTMTPGLHAAACTLIAYVRPFVIN
                     VLTPKDSTEFNYREPSPKAMQWTPYAVYVFVLTLLHHGYMVFLEWLDVGNFLDFIIKV
                     IATTGISLLLIFMVELLFPRQLKFRTNTA"
     assembly_gap    12154..12168
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3602 a         2709 c         2535 g         3446 t
ORIGIN      
        1 tttccaattt cagatttttt gactttgata ttgggatcat accagtcata tttcaccatt
       61 agctggtgtt tattactgat gatattctga aggaaaagca gaaaaattcc atcgaaatgc
      121 ctgacataag taggtaccgg attgccaccg gaagtaggta aggttcccgg gttactcgtt
      181 gccagcgatg tccccggttg agtaccaaac cagtattctg ccctccattc tgattcaccc
      241 caaccatgtt gcaactttac ctggatatct cccccgtagt aatgccgggg agatgatttt
      301 cccaggtttc gttcggaaga atcaacaaca aaaaccttat cgccattagc agcaaccccg
      361 ctttcataca catactttgt tccattcttc cagccacctc taagaaatga cagccctccg
      421 gacaattccc attttccggg tgagaaaggt cttataaaca ctcgggaaat aagatcctta
      481 taactgtcaa aatcagtagt gcctgaaaga ccctgtccat taaaaaaacc aatatcgaat
      541 ttgaacctgt ttcggctttt tcctttttcc tgtggctcat gggaaaccat aattccaata
      601 tccctttcag atggcatcaa tatttgcgac attcttcctc tttcgggtgt ttctctaaat
      661 gcagaagaaa ggtttacttc atagccaaaa ggacgggcaa acaaaccagc tgtcagcgaa
      721 aaaaggtttc gttttatctc aaataatttg agaaacatat cccggacaat tactcctctt
      781 tctgttgcgt ctatttggaa agtgaaaagc gctttgggat aacgaagttt agagggcaat
      841 atataatcaa tttttatcct tgctctgcgg agcatgaagc ggctactgga atattgagaa
      901 aaatttccgc cagtatagga aggagccccg tctgactggg ccttttgaaa ctgcggctgt
      961 atgtacccgg ttatccgaag gttttcaaac cgtttgatca ccggtctcac ggtatctttt
     1021 ataaaaaaag acgaannnnn nnnnnnnnnn nnnnnnnnnn caaggtctga cagatagcgc
     1081 tgagcataac catcagaagc tgtgagtaaa agggaaacta cggcaaataa gaacttattg
     1141 attgtaaatt ttgccggaaa catggtattt tcaaaattgc tgcaatataa agcgtttgtt
     1201 attgaatcgt taccttaatt ttatttgtat attaataaat tattaattct tccggcccgc
     1261 cgctaccccc aaacagtacc cgtattttcg caaaccatcc cgccgttggc gggaactaaa
     1321 aaaccaaacc aatggcagga cttaactcat ttctgaaaat cttcatgccg aagaacaaaa
     1381 ttttctatga gctttttgaa aaagtagcta acaatgtggc cacaatgggt tccattttaa
     1441 gggatcttgt ggcagaacct gattttgata aacgagccgc tttgatcgcc caactggagg
     1501 acctggagca tgccaatgat gagcataccc acagcctttt taccgaactg ggaagaaact
     1561 ttattactcc ttttgaccgg gaagacatcc attacctcgc cacatcacta gatgatattg
     1621 ccgactacat ttatgccgcc ggcaaaaaga tcaatttcta ccgggtaaac cccaatgata
     1681 cgggtatgca gaaatttgcc gaattaatag agcagggcgc tgaacaagtg aaaattgctg
     1741 ttaccgaact gcgggatatg aagaatatgc gcagcattac cgaagcattg gtaaagatca
     1801 atagtatcga aaaccaggca gatgatattt ttgatttcag tatcgaaaaa ctctttgcta
     1861 cagaagatga tgccaaagag gtaatcaaga aaagagagat ttaccaggta atggaaatag
     1921 tgaccgacaa atgtgaagat gccagtaatg tgatagaatc tatcatcatt aagtacgctt
     1981 aatccaatcc ttcacttttt tgccaacaac ttatgacacc ttttctcatc accataatag
     2041 ccctggccct gatatttgat tatatcaacg gttttcatga tgcggccaac tccattgcca
     2101 ccattgtatc aaccaaggta cttacgcctt tccaggcagt actttgggcg gcagtattca
     2161 attctgttgc cttttttatt ttcaaagacc acggggtagc cgatactgta gccaaaacag
     2221 taaagcctga ccatattgaa gggaatatga tggtagtcat tttttcaggt ctcattgctg
     2281 ccattacctg gaacctgctg acatggtggt tgggaattcc ttcatcctct tctcacacac
     2341 tgattggtgg ttttgccggc gctgcgattg ctgcaggagg atttggtgcg gtcaactctc
     2401 ccattattat caaaacagcc agtttcattt tcctggcccc gatcattggt atgatcatgg
     2461 cgtttattat gtcgctttgg ttcttaagct ctttcaaaaa aggctggacc tccaaaatat
     2521 tttccatcgc cattattgtt ttggtcggag tgttcctgtc taccaacctg gaatttgaca
     2581 ggacgatatt ggccaagaag actgattatg aatcatacat tgcccaggta atcttttatt
     2641 ccaagaactt caagtggatc ctgttagcat tcattttgct ggtaatggct gtttttgcct
     2701 tatacctcag tacactgaat gcacataaat ccaaccagtg gtttaagcgt ttgcagctta
     2761 tatcttctgc agcatttagt atcggccacg gaggcaatga tgcacaaaaa gtaatgggta
     2821 ttattaccgc tgcactgata gcaggcggaa ccatcaccga ttttcatcaa atgcccgatt
     2881 gggtacctat cgcctgttat gcggcaattg gcctcggtac aatgagtggc ggctggaaga
     2941 tcgtaaagac aatgggcacc aagattacca aggttactcc ctttgagggt gtggctgcgg
     3001 aaacagccgg agctgtcaca ctgttcgtaa ctgaagcatt gaaaattcct gtaagtacta
     3061 cccataccat caccggcgcc ataatgggtg tgggtgctac caaaaggctc tcagccgttc
     3121 gctggggtgt tacattcaac ctgatctggg cctggatatt aaccattcct atcagcgctt
     3181 tattagctgc cggcgtatat agtattgtaa gtctctttta atacaatcac ctttttaccg
     3241 taaaaaatcc tgacttttgt caggattttt ttatggcaaa gattcttgtt actggcggat
     3301 gcggctatat tggttctcac actatagttg acctggtaca aaacgggtat gatgttattt
     3361 ctgttgataa taactcccgt tccaaccctg ttatcctgaa aggggtggag cagatcaccg
     3421 gaaaaaaaat aaagaactat aaggttgatc tgtgcaattt tgatgacaca tttgccattt
     3481 tccaggaaaa taatgatatc agtggtatta ttcattttgc tgcttataaa gccgtaggcg
     3541 agtcggttga aaatcctttg ctgtattatg aaaacaatct tgtttcactg atcaacctgc
     3601 taaaatgtgt gcaggagttc aggatacctc attttgtttt ttcttcctct tgtaccgtat
     3661 atggtaaccc ggatgaggtt ccggttacag aatccactcc cttaaaacct gcggaatcac
     3721 cttacggagc tactaagcaa atgggtgagc agatcgttca ggattttgct aaaaacggga
     3781 ccaccagttg tgtgttgttg cgctatttca acccggcagg tgcccatcca actgctgcta
     3841 tcggagaaat gccgcttggc aaaccccaga atcttgtacc agccattacg caaactgcca
     3901 ttggacgtat tggtaaaatg tatgtacatg gcaatgacta tcctacccgg gatggctcct
     3961 gtatcaggga ctacattcat atctgcgatc tggctaatgc ccataccctt tcacttaaac
     4021 atattgaaga aggaaaaaat aaaagcagct gcgaggtatt caatttggga acaggtaatg
     4081 gcatcagcgt aatggaagcc attcatgcgt ttgaaaaagt aagcggcgta aaactgaatt
     4141 atgaaaccgg ccccaggcgt cccggcgatg tggtagccgt atatgccaat aatgacctgg
     4201 ctaaaaatgt attgggatgg aaccctgttt tttcccttga tgatatgatg agcactgcct
     4261 ggaaatggga attgcgtttg aaagccgatg aaactgtttt taccagtcag ccgggagaac
     4321 tgaactaaat attttcttct cgtaattcgc tatacagcat tcaccccgaa ccattacttt
     4381 tgccccactc aaactatagt aatgctgtta aaagacattc aatcaacagg cagtaaagac
     4441 accagaagtg gttttggtga tggcattgtg gaagctgcca gaaaaaacga aaatatcgtt
     4501 gccctaacgg cagatcttgc cggttcttta aagctgaacc agttcatgaa agagtttccc
     4561 catcgctttt ttcaatgcgg tattgcagag gctaatatga tgggcattgc agcaggtcta
     4621 actatcggcg gcaagatccc ctacactaca acttttgcca atttctctac aggcagggtg
     4681 tatgaccaga ttcgtcaatc tattgcctac agcggcaaga atgtcaaaat atgtgcctct
     4741 catgccgggc ttactttggg cgaagatggc gccacccacc agatactgga agatattggc
     4801 ttaatgaaaa tgctgccggg tatgacggta gttgttccct gtgattatgc gcaaacaaaa
     4861 gccgcaacta ttgccatagc agattatgaa ggcccggttt acctgcggtt cggccgtcct
     4921 tcatggccca tttttactga tggcaagctc tttgaaatcg gcaaagcaca gtttttttct
     4981 gaaggaaccg atgtaaccat ttttgcctgc ggacatttgg tatggaatgc tatccaggcc
     5041 ggtgccatac tccaggaaaa aggcatcagc gttgaagtga tcaatattca tactgtcaag
     5101 ccactggatg aagaagcggt gatcaactca ttgcgcaaaa caaaatgcgc tgttacagcc
     5161 gaagaacata atattattgg tggcttgggt gatgctattg ctcaatgcgc tacccggaat
     5221 tttcccgttc ctattgagtt tgtaggaaca aaagatactt tcggtgaaag cggaactcct
     5281 gcacaattgc taaaaaaata tggtttggat atacctgata ttgtagccgc agccgaaaaa
     5341 gtaatggcaa gaaaaaataa cgggtaatat ttaattttct ataaaacagg caggttcttt
     5401 taaatacgaa cctgcttttt tatttttaat aactggtaga cctgtttaaa caaattgctc
     5461 ttccaatatt ctaaccgaat caaagccctg accaatgccc ctgaccacga caagcgatag
     5521 tgagttgctc gtagaattcc gtaaccctgt tacgaaagag aaagcctata cccaaatcat
     5581 ccgtaaatac caggaaaaat tatactggca tatccgtcgg atggtgattg aacatgaaga
     5641 cgccaatgat gtgctgcaga atgtgctgat ccgggtatgg aatggactgg aaaatttccg
     5701 ggaagacagc caattatata cctggcttta ccgaatagcc accaacgaat gccttactta
     5761 cctggaacag cagaaaaaga gaacagctgt cagcctgagt gatgtagaaa caggcctgag
     5821 caacaagatc aaggcggacc gacattttga tgccaataaa ctggagtgga aattacagct
     5881 agctatgcaa caacttcctg aaaaacagcg tatagtgttt aatcttaggt attatgatga
     5941 gatgccttac gaggaaatga gccgggtgct tgaaaccagc gaaggcgcct taaaagccag
     6001 ttaccatcat gctgttaaaa aaatagagga ttatatgcta aatcattaaa ctttggaggt
     6061 tgacagctat cttataaacg caaaatgaca cagagagaca acatattgca ggaattgaaa
     6121 gaactggaca gccgtctggc taatgtcgct tcacaaaacg tttacacagt tccaaggggg
     6181 tattttgata gcctcactga cgaggtaatg tcccgaatca gggcactgga agcaaataat
     6241 cctgctgatg agttgaatgc actttctcca caactgggca aattgtccaa agaaatgcca
     6301 tatagtcttc caaaagatta ttttgaaacg cttacggaca atgtactgaa aacaatccaa
     6361 acaggcggtt tgtcatccaa agatgaatta gctgctattt ctcctttact tagcagcctt
     6421 aaaaaagaaa ccccttacag cgtacccgaa ggatattttg aaagcttatc cccaaccagg
     6481 cgagaagaag aagtgcaacc gtctgccaaa gttgtttcac tgttcagccg cacctggttc
     6541 cgctatgcag ctgctgcagt agttgccgga ataattattt tggcaagcat ctctcttttc
     6601 accagtgaaa aagaacccgg tggaaaagca ttggccaaat ttacccgtga tgtaaaaaag
     6661 atggatgaac agcagaaaga tgatatgatc gatttctttg atgccggact ggatggcaaa
     6721 gaaactgcac aggtaaatac agacgccaaa aactcacaag aagtaaaaga attactggaa
     6781 ggtgtctcag aagaagaact gaaagatttc caggaacaaa cagaagcgat tgaagaagtg
     6841 ttaatgacaa attaagcaac aatgaaaaag tggttaatca tattattcct catccccggt
     6901 gttgcaacca tggcgcaaaa tgatgagccc actccgcagg agggtaagat acaagagcgg
     6961 atgagggaat atattcagaa caaactcaac ctgaacagga atgaagccga ccggttcaca
     7021 cccgtattcg ttcgttactt tcgtgaattt gcccagactc atcgtcaaaa caggggtgac
     7081 aggttagtgc tgcagcaaaa aatcgttgaa cttcgtttgc gctaccgcgg agaattcagg
     7141 caaatactgg atgagcaacg agctaataaa gtctatttat acgaagatga gttccgaaga
     7201 aaagccgttg atatcatccg tgaacacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnngg ctatcatgct ccggatgtac aattgccttt tgtagttatt
     7321 aatgtattaa tatttttagc tatcggaagc tggtttgaaa aaaccagctt ttttattgaa
     7381 aagtccacaa tggatgttta acatcatggt aaaaaagaaa ggtccatttt tcttttgctc
     7441 cctgctccca ccatttttgt cttaattcca ttgctttttt accattgtaa tcatatttgg
     7501 ccacaaagcg gtgtttcatt tgttgcaatt gtggcgcttc atcgcctccg aaaaagatga
     7561 tctcaccggc ttcttttacc caaaatacct ggtgatgcgg tgaatgggcg ccggaaacct
     7621 cataccggat gtattcatca ataatccctg aaccttcgag caaaacggcc tgttcattat
     7681 ttcgcaaaca ttccagttct tcctggataa aagaaggaaa ccctttttca aaagcataaa
     7741 ctaattcggt ccgttgcaca taatattttg cattgggcag ggaaagccgg gcacccaaac
     7801 catccctttc tattccaact gcaccggcat ggtctttatg caggtggctc atcaacactt
     7861 tggtaacttc tgatggatta ataccaacat tcatcaggtt ttggtgaagc tgcaagatgc
     7921 catttctttc gaaaccaagg cctgtatcca ataaaatgat gtcttttgag gtgataacaa
     7981 caaatggctg gatctctacc aataaactcc ctgaaggcct gtccttcagg ctatgctcgt
     8041 tttcgtcaaa cggtacaaaa tgtttggttt tatcaatggt aaaagtgcct tctgaaagtg
     8101 gaatgatttt cataaacttc aaaattacaa ttccagggct ctaaagttgg gatttggaac
     8161 ttgttaattg tcattcctat ctcaatacca tttgcctgtc ggcatccaaa cgaataagaa
     8221 tgaaaagcaa aatggtgaaa gtaagcagtg atgtgccccc gtagcttaca aacggaagcg
     8281 ggatgccaat aaccggtgca agtcccactg tcatggcaat attaatggca atatgaaaaa
     8341 agaaaacagc tgccaccccg tacgcatagc tcctgctgaa tgtactgcgc tgtcgctcgg
     8401 caatcgtaac aatacggaaa agcaggatga gataaagacc caacaacaca aagctgcctg
     8461 cgaaaccaaa accctccccc acagtacaaa aaataaaatc cgtacgttgt tccggtacaa
     8521 aatcgtagcg ggtttgtgtt gcgtttaaaa ttcctttccc aaatatcctg ccgctgccaa
     8581 tggcaatttt tgactgccgt acattatagc tggttccgct ttcctttttc ttttttaatt
     8641 cctctgcagt gagctcagca cttttatcga gataaggatt gtccttacca aacaaatcat
     8701 aaatcctttc tacctggtgc ttttgtaaaa cgtttttaaa agcgtaagga acggcaaacc
     8761 gctgaagccc cacacaaagt acccataaga aaataatagt cgtcaggatc tggcgacggc
     8821 gttttatctg ccggcggaat atgtaaatca gcaaggcagc aagcaccgtc aacgctatgg
     8881 ccagcgtatc gggctcaatc acaatggtag caacaaccag ggctgtcacg gcaataccga
     8941 tcaacagaat gacagaaggc aacccttccc tgtacattac aatgaagaag gagaaataga
     9001 ccagggccaa accggtttcg ctttgcaata aggtaagggc tgcaggaaac aaggcaatac
     9061 ccgcagcaat taactgcgcc cttgtttttg taaaatccgt ttccggtctt gagaggtatt
     9121 tggccaatgc cagcgccaca aatattttac acagttcagc aggttgaaaa ttaaaagctc
     9181 cgagcttgat gattgactcc gttcccttta ttggtgaatg aaaagggaac accagcagca
     9241 acataaatat tcctaatgcg tatcccaaat ttgctgtagc agcaaaaaac ttactgtcgg
     9301 tgagcagtat aaagaaacca attaaagtac ccaggcaaaa aaagtagaac tgtttgctgt
     9361 aatccgtctt aaagcgaaag aagctttgaa tgatgggatc tccttctttg taagtaacag
     9421 cgaaaatggc tgtgagacca ataaccacca gcaacaggta aatacctatc aggctccagt
     9481 ctattccttt tgatatggcg gggttgcgct ggttcatgat aatacccgac tttttaatgc
     9541 ggaagccacc tttttttctt cttgcatcca gatatacgtt atcgtctttt gcttgtctgc
     9601 cggtttgggt gcaggaggtt ttggggtatc agcctgtgcc cttctgaatt tcttaatata
     9661 actactgtcc tttgtcattt tgaaccactc ccatgcccgt gaagaatctg ccttatactg
     9721 taatcgcttg taatgggcag gtatcaggtt ggcttttgaa attctgtcta catctgcttc
     9781 actttttttc tggattgtgt cgttcaggta cttttcaata agtattcttg atattggacc
     9841 accccaggta gccccgtaac cggcattctg cacacaaaca gcgatggcaa ttttcgggtt
     9901 gtcttttggt gcaaaacaaa caaacatgga gttattaggg agtttaattc tccggccatc
     9961 cagtatcgta tagttttcag cggtaccggt tttggcacaa acttctattc cgggtatttg
    10021 tgcaatcctg gctgtaccaa attttaccac atcattcatt ccttcgataa cggcacgata
    10081 agctgtatcg ggaatatgtg tcaatacttc atgcttcacc ctgaacttgt tcatgtatac
    10141 tgtatcatcg tccgtttcat cgtctatctt ctgtacaaaa tggggtgtat aataataccc
    10201 tttattggca atgatacaca ttgcattggc caactgtaat ggtgttgcct gcatctgatc
    10261 ctgtcctata cctagtgtca gattggtaca ggagttccag ctgccacgat attgtttatt
    10321 ataactggct gtatcaggaa tattcccttt gttttcactg ggcaaatcca cccctaaacg
    10381 gacacccaac ccaaaaaggt tcatatactc cctccacttc atgtatcctt tcttaacccc
    10441 gccatactga gggttgtcag cagccatgcg gtagagatgg gtgaaataag aattacaaga
    10501 atacgcaatg gcatttcgta aattggcgga atgcccacca ccggaatggg tacaggcagg
    10561 tttacccgaa ccacagccca tatagcgacc tccgcaggga taaccataag caggagttat
    10621 taatccttca tcaagagcga ccaatgctcc aagaggttta aatgtggaac cgggttcata
    10681 taatccatta attgccctgt taagcatcgg acctgccaca tccaactgca ggcggccgaa
    10741 atttttactt ccttccggac cagtcaaatc attgggatta taatttggcc ccgataccat
    10801 agccaatatt cctcctgttt ttggatcaat agccaccagc cctcctactt tgttcgtcat
    10861 taatttctct gccagttgct gcaggtctgc atccaggtat gttcttagcc ctctacccgc
    10921 aacagccgca gtatcaaaag aacctttttc ataatggcca actaacctgt tcttattatc
    10981 cttgatccag tattccaccc ctctttgacc catcagcact ttttcataat acgcctcaag
    11041 tcctgtttta cctatataat ccccctgccg gtaaaaattt cccgactttt ctatatcctt
    11101 tggatctgcc tctcttacat agcctaaaat atgcgcagct gcgttaaaag gatatgttct
    11161 taccggtctt tccaccagcg taaaaccggg aaacttccag ctattttctt caaatcttgc
    11221 ttgaatgtca ggcgttaact gatcatcaaa aattgaaggc cgcaccgaag tgtttttgat
    11281 cttggcttcc agtattcgct tgttgaactc tgccgtatct aaattcatca gccggcaaaa
    11341 ctcctgcaca tctatgccct tgatctctgc tggcgttacc atcagatcat aactgatagt
    11401 attattaaga atggctttcc cttccctgtc ataaatgatt cccctttccg ggtaaatgat
    11461 ctttggataa acagcattat ccattgccag ttgcctgaat tttcctgaca tgacctgtag
    11521 gtaaaacaac tgacctataa taaccaaaaa tgtaacccca aatatcaggc ggatgatacg
    11581 gcttcttgat tggttaaaca cggacatatt aaactatggg ataaactgtg cgaggtatca
    11641 ggtgaaaaat aaagtacaag gctgcaaagt acgaattacg ataaacggtt tccaaatcta
    11701 attgacctga aaagattttt tttaaggaaa atttcagtac tgcctgttga tttttctatg
    11761 ccgtattggt gcggaatttc aattgtcggg gaaacaataa ttcaaccata aaaatcagca
    11821 gcaaactaat gcctgtggta gcaataactt taatgataaa atccagaaaa ttcccaacat
    11881 ccagccattc aagaaatacc atatagccat gatgcaggag ggtaaggaca aaaacataaa
    11941 cagcataagg tgtccactgc atggctttgg gtgagggttc ccggtagttg aattcagtac
    12001 tgtctttcgg cgtcaataca ttgatcacaa aaggccttac ataggcaata agggtgcagg
    12061 ctgctgcatg caggccgggt gtcatagtaa aataatccag tgtcagtccg gtaacaaagc
    12121 ctatgaccag caagccaaag cgggaaatgg taannnnnnn nnnnnnnngg caaccaaaga
    12181 atgaaaagaa aatagatata aggtgtaatg aacctatgca ggtggggcat cttattcaga
    12241 atatacacct gtaccaggat gaatacagca aatcttatta tgtttcttag cagatcactc
    12301 acttcggtgc ttttttacct tgttcaattt tgtttttggt gtcctgcatc agttgccact
    12361 gctcatctct ttccacattc tct
//
LOCUS       DAORLS010000057        12347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2739_length_12347_cov_1.977628, whole genome shotgun sequence.
ACCESSION   DAORLS010000057 DAORLS010000000
VERSION     DAORLS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2739_length_12347_cov_1.977628"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    249..386
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<387..739)
                     /locus_tag="PK977_06250"
     CDS             complement(<387..739)
                     /locus_tag="PK977_06250"
                     /inference="COORDINATES: protein motif:HMM:NF026142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HRF17748.1"
                     /translation="MECLKQQSPQAIKKIDGVKQATIAGSIRRKKDIIGDIDIVITAD
                     ERKWKKIITAITQLPQVETVIAAGKTRASFVLKTNQVQADIRVVHEDEYGAALFYFTG
                     SRDHNIQLRSLAKQRG"
     gene            complement(693..1295)
                     /locus_tag="PK977_06255"
     CDS             complement(693..1295)
                     /locus_tag="PK977_06255"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF17749.1"
                     /translation="MKRADNQKMHSNKELSRLFSQMADCYRYLGPDERFRANAYAGAA
                     QTLRNLQEPVENLADDMQQLEDLKNIGKSIAEKIIEFLATGQIQTFEDLKKKVPFALL
                     ELMDIEGFGPATLRLLHDELGITTKETLITAIEKGKLEKIKGFAFRKIEKLKQALKLE
                     SSKKRLPLKEAQRIADNLLQSVKSIDGVLKATIAAGDKKN"
     gene            1425..3518
                     /gene="ppk1"
                     /locus_tag="PK977_06260"
     CDS             1425..3518
                     /gene="ppk1"
                     /locus_tag="PK977_06260"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRF17750.1"
                     /translation="MAEEKKIVFLNRDLSWLSFNHRVLQEAADRSVPLYNRIAFLAIF
                     SSNLDEFFRVRMPSIFAFTSIEAKKTTLREEYPKELVQIVQNMVLEQQEEFGSILREQ
                     ILPELQQNHICLYYNQPIRAEHTETIREYFLSKVLSFLQPVILQKENQATIFLENNAL
                     YFIVDIEANDQPDKHQYALLNIPSGNLRRFSELPMLGDDHYLVFLDDVVRENIEEVFP
                     GFKVHGLYSVKLTRDAEMNIEDEFIGDIAEKIEKQLEKREAGLATRLLYDKTMPAEVK
                     DFVQKYFMLRKQEMVEGGRYHNLRDLGGLPNPLRGQLTQANWPAVAHPGFDNHRSIFQ
                     TIAEKERMLHLPYHSYNYILRFFNEAAIDPGVKEIYVTLYRVAADSHIVNALISAAKN
                     GKKVTVFVELKARFDEANNLKWSKKMKAAGIRIINSIPRLKVHAKVALIKRQEDQQDK
                     WYSFMATGNFNEATGRFYTDHVLFTFNQDFGNELMPLFDYLQSRKQPAEYGKIPFKHL
                     LVSQFNMIKRFSQLIDREIDNAKAGKPASIIIKLNNLQERAMIARLYKASKAGVKVHL
                     IVRSICCLAPGVPGQSENIVVHRIVDRYLEHARVFAFHNNGEPEYYMGSADWMNRNLH
                     SRIEVCFPIYDKQMGEELQHILDLQLKDNKKAVLLSSDVQTGAANNIRVLSAGQEPLA
                     AQQAIYDFVKSVTAR"
     gene            3700..4338
                     /locus_tag="PK977_06265"
     CDS             3700..4338
                     /locus_tag="PK977_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17751.1"
                     /translation="MQKESTSQTVYLAGSFNYWHAGDSLYRMKKIGDNRYTLTLPVFD
                     NHSYHYKYTLGHWNRVELAANDSNINNRVFESANGKQITDTVIKWKALMPASAQTNPQ
                     MQQINAMKDSALAKLQPELNNMIDFLKQYVQNFLKAEPSASLHRRLDKKVEKKIGSAY
                     RQVTKLLWDVFASISPEQKKQMNELLNKPEAQKDFINALGNAFGTVVEGKKQ"
     gene            4509..>4761
                     /locus_tag="PK977_06270"
     CDS             4509..>4761
                     /locus_tag="PK977_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRF17752.1"
                     /translation="MRKLLILFVAGISVLSVKAQSEKIPLLFRLDDIGMCHSVNMAAK
                     EVLETRMPVSMSVMVPCPWFTEAVEILKQYPHVSVGIHLT"
     assembly_gap    4762..4820
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4821..5372
                     /locus_tag="PK977_06275"
     CDS             <4821..5372
                     /locus_tag="PK977_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRF17753.1"
                     /translation="VDSLGHFFPSRSKLFANNPRLEEIETELRAQIEKAQKAGLKIDY
                     LDYHMGAAMQTPQTRAIVEKLAAEYGLAISRYYDEVDVEGGYAAPVANKLDTLLKKVT
                     HLQPGGTKLFVVHIGLDEPEMAAMEDLNPSGPKDMSKHRQAELRALLALPFQQLIYND
                     RYRIVNYRMLNEEKGLKNMKRPG"
     assembly_gap    5710..5799
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5800..>6100)
                     /locus_tag="PK977_06280"
     CDS             complement(<5800..>6100)
                     /locus_tag="PK977_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17754.1"
                     /translation="PAGTYTYNWFRNTVAIPGASNTLGVDINGLGSYTVQATNAAGCS
                     NTSNTLVIADSATSRLFIMPNPNNGQFEVSYYSPAATSFTLTISDSKGAVVYSKAY"
     assembly_gap    6101..6182
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6183..>6853)
                     /locus_tag="PK977_06285"
     CDS             complement(<6183..>6853)
                     /locus_tag="PK977_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17755.1"
                     /translation="PVPTASITPSGPTTFCQGNSVTLVASAGASYLWSNGATTQAITV
                     ATSGNYSVTVTNAEGCSATSAATTVTVNANPPAVITASGPTTFCNGGSVTLTANSGVS
                     YLWSNGATTQAITVSGPLGATNYSVQVTQAGGCVSSSPVTTVTVNPVPTATITASGPT
                     TFCEPNNVVLTASAGSSWLWSNGATTQAITVSSPAASGNYTVTVTNSSGCSATSAATS
                     VTVNP"
     assembly_gap    6854..6984
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6985..>7256)
                     /locus_tag="PK977_06290"
     CDS             complement(<6985..>7256)
                     /locus_tag="PK977_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17756.1"
                     /translation="QGSSVTLVASSATSYLWSNGATTQAITVGTSGNYSVTVTNSFGC
                     SAASAPTAVTVNPNPQATITASGPTTFCNGGSVTLTASAGTSYLWS"
     assembly_gap    7257..7418
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7419..>10212)
                     /locus_tag="PK977_06295"
     CDS             complement(<7419..>10212)
                     /locus_tag="PK977_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF17757.1"
                     /translation="SGVNVQALSAFPLRSIFQLDEKQKMHPALYRGEIPPHAVNQFGY
                     IDVSVITYERISAAAVMNSIQSVGGSILADVPVFKTLRLRIPQTAVQMLAGLPYVQWV
                     EAIDPPNVLENLPGRTLHRVNILNDGVRNLKGDDINIGIWDGGPVDQHLDFSPAGRLT
                     IIEAGSPIQHATHCAGTITGKGLINPTARGMAPNARLFSWNFSGDIQSEMTTGVPANN
                     LLVSSHSYGSSSSPNCDINGPLLTYNTTARNTDIVLNTNSNHLQVHSAGNSAGVCPNG
                     YLTITGSGKPAKNNLVVANITSTEAWSSSSSSGPVQDGRIKPEISAMGNAVFSTSTPT
                     NTYATLSGTSMAAPGVAGSSALLYQRYKQLNGNAIPPSTLIKNIICNGAQDLGNPGPD
                     YKFGFGRINALTSVRILEDNRYVVNSVANGASNDVTITVPAGAARLKVMLTWNDPAAT
                     ANANPSLINNLDLTVNEGANTTLPWILDKSNPIANATRGVDNYSNIEQVTLDNPTAGT
                     YTLRVNGTAVTTGPDQPYALTWTIDQPYIEVIYPNGGESFNPGSSQVITWDNAGVTGN
                     QTVEYSLDAGGTWTTIGTVAANVTRLTWSVPAANTSTARVRVTSGSLTDASDNNFNII
                     GTPTGLNTSSATCNAGEVILNWTAVANATQYDVYRLDATTGNYVLAVPDVTGTTATLT
                     GLTPGSTVWMYVVAKNSTTGAIGDRPTAVSGVVSSGGGGLGVIGSITGQQNICGTPSG
                     VPYSIAAVSGATNYTWAAPPGAIIASGQGTTNITINYPGGSTNGNVSVFASNATCQSS
                     TVTLPITIGGTSVPSPVSGGPQTANLCPGDPVPTLTATATVPGGYTLVWYNAPTGGAI
                     VASPTLSAPGTVTYYASARDNNTLCESITRTPVTLTITQVSSATITASGPTTICQGSS
                     VILTASAGSSWSWSNGA"
     assembly_gap    10213..10238
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10239..10500)
                     /locus_tag="PK977_06300"
     CDS             complement(<10239..10500)
                     /locus_tag="PK977_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17758.1"
                     /translation="MKRFFTLSVKTLFFLLVPGYLFAQDFNVQLHAGKFIPQENIQTI
                     TKSDPVFLRSLFNGKHYVTIQLKTIPSQTLKDQLKASGIELID"
     gene            10669..11571
                     /locus_tag="PK977_06305"
     CDS             10669..11571
                     /locus_tag="PK977_06305"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRF17759.1"
                     /translation="MAFEWKGVFPALLTPFNQNEELDLQMYEKNLQAQLDAGVHGIII
                     GGSLGEASTITEKEKEELTEFSVEYINGKIPVILNIAESTTKEAIQQAANAKRWGADG
                     LMLLPPMRYKTDDRETITYFKAVAHSTDLPIMIYNNPVDYKIDTRPDMFDELVECANI
                     TALKESSRDVTNLTRMKNRFGDRFKILCGVDTLAMEELCLGADGWVAGLVDAFPAETV
                     AIYKLVKAGKIEEAAKIYRWFMPVLELDLHPKLVQYIKLAAMQTGIGSEYVRAPRLQL
                     EGTERQNVLQTIDEAISNRPKLVF"
     gene            11593..>12347
                     /locus_tag="PK977_06310"
     CDS             11593..>12347
                     /locus_tag="PK977_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF17760.1"
                     /translation="MFKDATTDEIDSALRKAWQAFHLYRKFSLKQRADFMKAIAVELE
                     NTGDSLISTAMRETNLPEARLRGERARTIFQLNCYAEACVKGDWLEARIDTAIETKIP
                     PKPDIRKMLVPLGPVVVFGASNFPLAYSTAGGDTACAFAAGCPVIVKAHPAHPDTSEQ
                     VAHAIKNAVQKCNMPDGVFQHIQGNSFEVGKALVMHPFTKAVGFTGSYTGGKQLYDWA
                     QQRKEPIPVFAEMGSINPVFLLPEKLSKEADTIA"
BASE COUNT         3245 a         2528 c         2810 g         3076 t
ORIGIN      
        1 ccagtaaact gatagaagga tcttccatcc ggtggtgacg aaaatggtta agtagtaatg
       61 gcaatttcag gaactggtgt agttctgtta caccgttcaa caaaagaaca gccaatataa
      121 tggtgaatat ttttttcatt gccagaccgg aaagtaagat tcaaacggga aatacaaggt
      181 gatttttatc acccgcctgt taaagtttat aaaaagggtc agttgttcat tgcctggttt
      241 aactcattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnncctc gttgtttggc aaggctgcgt aactgaatat
      421 tatggtctct acttccggta aaataaaaca gggctgctcc atattcatct tcatgcacaa
      481 cccggatatc ggcctgaact tgatttgttt ttaatacaaa gctggcacgg gtctttccgg
      541 cggcaatcac tgtttccacc tgtggcagtt gggtaatggc ggtaatgatc tttttccatt
      601 ttctttcatc ggccgtaatt acaatatcaa tatcaccaat gatatctttt tttctgcgga
      661 tactaccggc gatagtagcc tgttttacgc catcaatttt ttttatcgcc tgcggcgatt
      721 gttgctttaa gcactccatc tatacttttt acagattgca ataaattatc agcaatacgt
      781 tgtgcttctt tgagaggcaa ccttttcttg ctgctctcca gtttaagggc ttgtttcagt
      841 ttttcaatct tacggaaagc aaagcccttt atcttttcca gtttaccttt ttcgatagca
      901 gttataagtg tttcttttgt tgtaatgccg agttcatcat gcagcaatcg cagggtagcc
      961 ggtccaaatc cttctatatc catcaattcc aataaagcaa aaggaacttt ctttttcaga
     1021 tcctcaaatg tttgtatctg tcccgtggcc aggaattcta tgatcttctc tgcaatgctt
     1081 ttaccaatat ttttgagatc ttcaagttgt tgcatatcgt ctgccaggtt ttctaccggt
     1141 tcctgcagat tgcgcagcgt ttgtgctgca ccggcataag cattcgccct gaatctttca
     1201 tcgggaccta aataccggta gcaatccgcc atctggctga atagccggga cagttctttg
     1261 ttgctatgca ttttttggtt gtcagccctt ttcactgtag caaggtaggt gaccctatga
     1321 gcagtaaaaa atgagccctg tcaggcagtt aactgtttcg ggtactatct tcgccacttc
     1381 gcacaaaacc attatcttag ttcacttaca accaaaactt acctatggca gaagaaaaga
     1441 aaattgtttt tttgaacagg gacctcagct ggcttagttt caatcaccgg gtattgcaag
     1501 aggccgccga ccgatccgtt cctttgtaca accgcatagc cttcctggct attttttctt
     1561 ccaacctgga tgaatttttc agggtcagga tgccttccat ctttgctttc accagtattg
     1621 aagccaagaa aactactttg agagaagaat atcctaaaga actcgttcag attgtacaga
     1681 acatggtgct ggaacaacag gaggagtttg gcagtattct acgggagcag atattaccgg
     1741 aactacagca aaatcatatc tgcctttatt ataatcagcc catccgtgca gaacatacgg
     1801 aaaccatccg tgaatacttc ctgtcaaagg tgttatcctt tctgcaacct gttatcctgc
     1861 agaaagaaaa ccaggctact atttttctcg agaataatgc tctttatttt attgtagata
     1921 ttgaagccaa cgatcagccc gacaaacatc agtatgcatt attaaatatt ccttcgggca
     1981 acctgagacg tttttccgag ttgcccatgc tgggtgatga tcattacctc gttttcctcg
     2041 atgatgtggt gagggagaat attgaagaag tatttcccgg tttcaaagta catggcctgt
     2101 acagtgtgaa gcttacaaga gatgccgaaa tgaatattga agatgaattc atcggggaca
     2161 tcgcagagaa gatagaaaag caattagaaa aaagagaagc cggtcttgct acccgtttac
     2221 tatacgataa aacgatgcct gccgaggtga aagattttgt tcagaaatat tttatgttgc
     2281 gcaaacagga gatggtagaa ggcggccgct atcataacct gagagattta ggaggattgc
     2341 ccaacccgct gcgtggacaa ctgacgcagg ctaactggcc tgcagtagca catccgggct
     2401 ttgataatca ccgttctatc tttcagacca ttgctgaaaa agagaggatg ctccacctgc
     2461 catatcattc ctataattat atactccgct ttttcaatga agcggccatt gatcccggtg
     2521 taaaggaaat atatgtcact ctgtatcgtg tggcagccga ttcgcatata gtgaatgcac
     2581 tcatcagtgc tgccaagaat ggaaaaaagg tgacagtgtt tgtggagttg aaagcaaggt
     2641 ttgatgaggc caataacctg aagtggagca agaagatgaa agccgccggt atccgaatca
     2701 tcaacagcat accgagactg aaagtgcatg cgaaagtggc actgatcaaa cggcaggaag
     2761 accagcagga taaatggtac tccttcatgg ctacgggcaa tttcaacgaa gccaccggcc
     2821 gtttctacac cgaccatgta ctgtttactt tcaaccagga ttttggcaat gaattgatgc
     2881 ctctcttcga ttacctgcaa tccaggaaac agcccgctga gtatggaaag atacctttca
     2941 aacatttgct ggtttcgcag ttcaatatga tcaaacgctt cagccagttg attgaccggg
     3001 aaattgataa tgccaaagca ggtaaacccg ccagcatcat catcaaactg aacaacctgc
     3061 aggaaagggc catgatcgca aggctatata aagccagcaa ggcaggagtg aaggtacatt
     3121 tgattgtacg cagtatttgc tgcctggcgc cgggtgtgcc ggggcagagt gaaaatatag
     3181 ttgtacaccg cattgttgac cgctacctcg aacatgcaag ggtcttcgcc tttcataaca
     3241 acggggaacc ggaatactac atggggagtg ccgactggat gaaccgcaac ctgcacagcc
     3301 gcattgaagt gtgtttcccc atttatgata aacagatggg cgaagagctg cagcatatac
     3361 tcgacctgca actgaaagac aataaaaaag ccgttctgct ttccagcgat gtgcaaacag
     3421 gcgctgccaa taatatacgg gtattgagtg cagggcagga gccgctggcg gcacagcagg
     3481 ctatttacga ttttgtgaag agcgtaacgg cccggtagtg ctacggctga caggcgtcat
     3541 tgcgttgcgg caaatactct tttactttcg caaaaaattt tcttatgcgg gcaagtataa
     3601 gatgttgttg tttgctattc tatctattgc tgttttcagc aagcagcaac gcacaggtag
     3661 cagccgtcac cttcacggtg caggtaccat ctatcgatag tgcaaaagga gtctacctcg
     3721 cagacggttt acctggcagg atcttttaat tactggcatg ccggcgattc attgtaccgc
     3781 atgaagaaga tcggcgacaa ccgttatacg ctgacgctcc cggtgtttga caatcattct
     3841 taccactaca aatacaccct tggtcattgg aacagggtag agctggcggc caacgacagc
     3901 aatatcaaca accgtgtatt tgagtcggcc aacggaaaac agatcacaga tacggtaata
     3961 aaatggaaag cactgatgcc cgcctctgca cagaccaatc cgcaaatgca acagatcaac
     4021 gccatgaaag attctgcgct ggctaaactg caaccggagc ttaataacat gattgatttt
     4081 ttgaaacagt atgtgcagaa ttttttgaaa gcagaaccca gtgcttccct gcaccgcagg
     4141 ctcgataaaa aagtggaaaa gaagatcggc agcgcctacc ggcaggttac taaattgttg
     4201 tgggatgttt ttgccagtat ttcgccggag caaaagaaac agatgaatga actgctgaat
     4261 aaaccggagg cacaaaagga ttttatcaat gccctcggca atgcgtttgg taccgtggta
     4321 gaaggaaaga aacagtgaaa tgaaaaaatt gtattgcttg tctgtgtcac cctgatctca
     4381 gtttaccctg aggaatcgaa aggtcgaagc atagacagtc gaaagatcac cctatgacaa
     4441 gctcagggtg acaacagttt gctgatagtt ctgtacattg aatattattt tcaatttaaa
     4501 ccagttgtat gcgaaagctg ctcatccttt ttgttgcagg catttccgtt ttatctgtca
     4561 aagcacaaag cgaaaaaata ccgttgttgt tccggctcga tgatattggt atgtgccatt
     4621 cggtgaatat ggccgccaaa gaagtgctgg aaacccgcat gcctgtttcc atgtcggtaa
     4681 tggtgccctg cccctggttt accgaagcag tggaaatact gaagcaatac ccacatgtat
     4741 cagtgggtat acatcttacc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn gttgattcat tgggccattt ctttccctcc cgcagtaagt
     4861 tgtttgccaa caatccccgg ctggaggaaa tagaaacaga actaagggca caaattgaaa
     4921 aggcacagaa ggcaggtcta aaaatagatt acctcgatta ccacatgggc gctgccatgc
     4981 aaacacccca aacccgggcc atcgtagaaa agctggcggc ggaatatggc ctggccattt
     5041 cccgctatta tgatgaggtg gatgtggaag gcggctatgc ggcccccgtg gccaataaac
     5101 tggatacact gctaaagaaa gtaacacacc tgcagcccgg cggcaccaaa ctatttgtag
     5161 ttcatatcgg gctcgatgaa ccggagatgg ctgctatgga agacctgaac cccagtggcc
     5221 cgaaagatat gagcaaacac cggcaggcag agctaagggc cttgctggcc ttaccctttc
     5281 agcaactgat ctataatgac cgttaccgca tcgttaacta ccgtatgctg aatgaagaaa
     5341 agggattgaa gaatatgaag aggccggggt agtggggaaa ttgatagagg aaaatagaga
     5401 attggttttg ttataagtct gatttaattg aagttgactt aaaaagcgat ttcgcatttg
     5461 aacttgttga aggatttgtt ccattttgac aaacccactg ttccgtttca tcgcaacaat
     5521 ttgtgttaat ctgtggtcaa ataaaaaaag attccggcta aggcaggaat gatactactc
     5581 cataatcaaa atccttacta aaaaagcccc gacaattctg ccggggcttt gtatgtacta
     5641 tactaatctc aataagcatt aatgatgcac catcactcta cccaccgcta tgcgtttgcc
     5701 gttgcggtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gtaggccttg ctgtacacta
     5821 cggctccctt agaatcagag atggtgagtg taaagctggt ggccgccgga ctgtaatagc
     5881 ttacttcaaa ctggccattg ttggggttag gcataatgaa caatcttgat gtagccgaat
     5941 cagctatcac cagcgtattg ctggtattgc tgcagccagc tgcattggta gcctgcacag
     6001 tatagctgcc caggccattg atgtctacac caagtgtatt agaagcgccg ggtatggcca
     6061 ctgtattcct gaaccagtta taagtgtaag tgcctgccgg nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nntgggttta cggtaacact ggtagcagcc gaagtagctg aacatccgct ggagttggta
     6241 accgtaacgg tgtagttgcc cgatgctgcc ggtgaggaaa ccgtgatggc ctgcgtggta
     6301 gcgccattgc tccacaacca gctgctgcct gcagatgcag taagcacaac attattcggc
     6361 tcgcagaaag tagtaggtcc gctggctgta atagtagcgg ttggtaccgg gttaacagta
     6421 accgtagtaa caggcgaact gcttacacaa ccacctgcct gtgttacctg cacagaatag
     6481 ttggtagcgc cgagtggtcc gcttacggtg atggcctgtg tggtagcgcc attgctccac
     6541 aaataagaaa cgccgctgtt ggccgttaag gttacactgc cgccattaca gaaagtggtt
     6601 ggtccgctgg cggtgataac agccggcgga ttagcattta cggtaacggt agtggctgct
     6661 gatgtagcgc tgcaaccttc tgcattggta acggtaacag aatagttgcc cgatgtggca
     6721 acggtgatag cctgcgtggt agcaccattg ctccacaaat atgaagcacc ggcactggct
     6781 acaagtgtta cagagttacc ctggcagaaa gtagtaggtc cgctgggggt aatacttgca
     6841 gtcggaacag gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnngctcca gaggtaagaa gtacctgcac tggcggttaa
     7021 ggttacactg cctccgttgc agaaggtagt ggggccgctg gcggtaatag tagcctgcgg
     7081 gttagggtta acagtaacag cagttggcgc agaggcagcg ctgcagccaa atgaattggt
     7141 aaccgtaaca gaatagttgc cggatgtacc cactgtgata gcctgcgtgg tagcgccatt
     7201 gctccacaaa taggaagtag ctgaactggc aaccagcgtt acagaactac cttggcnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cgccattgct ccacgaccag
     7441 ctgctgcctg cagatgcagt aagaataact gagctgccct ggcagatcgt agtaggtccg
     7501 cttgctgtta tggtagcaga tgatacctgt gtaatggtaa gcgtaaccgg ggtacgggta
     7561 atgctttcgc aaagtgtatt attatccctt gcagaagcat agtaggttac tgtacccggt
     7621 gcacttaatg tggggctggc cactattgca cctccagtag gtgcattgta ccataccaat
     7681 gtataaccgc cgggaacagt ggcagtagcc gttagtgtag gtacagggtc accgggacaa
     7741 agattagcag tctgtggacc accactaaca ggggacggaa cagaagtgcc gccgatagtg
     7801 atgggtaaag tgacagtgct tgactggcag gtagcattgc tggcaaatac agaaacattt
     7861 ccattggtgc ttccaccggg atagttgatg gtaatattag tagtgccttg tccgctggcg
     7921 ataatagcgc cgggaggagc ggcccatgtg tagttagtag cgccggatac agcagcaata
     7981 gaataaggaa caccggaagg ggtgccacag atattctgct gaccggtgat ggatccgata
     8041 acgccgagtc cgccgcctcc tgaagacacc acaccgctga cagctgttgg cctgtcgcca
     8101 atggcaccgg tggttgaatt tttagcaacg acatacatcc acacggtaga acccggagta
     8161 agaccggtga gggtagccgt ggttcccgtt acatctggta cggctaaaac ataatttccg
     8221 gtagtagcat cgagcctgta cacatcatac tgggtggcat tggctactgc tgtccagtta
     8281 agtattactt caccggcatt acaagtagcg ctgctggtat ttaagccggt aggcgtacca
     8341 ataatattga agttgttatc actggcatct gtcaaagaac cactggtaac tcttacacgg
     8401 gcagttgaag tattggcagc aggtactgac caggtaagac gggtaacatt ggcagccact
     8461 gttccgattg tagtccatgt tccgccagca tccagtgagt attcaacggt ctggttacct
     8521 gtaacacctg cattatccca ggtaatgacc tggctgctgc cggggttaaa tgattcgccg
     8581 ccgttaggat agattacttc aatatatggc tggtctattg tccaggtaag cgcataaggc
     8641 tggtcgggac cggtagtaac ggccgtacca tttactctca gtgtataggt tccggcagtg
     8701 gggttatcca aagttacttg ttcaatattg ctgtagttat ctacaccacg ggtagcatta
     8761 gcaatggggt tgcttttatc caaaatccat ggcaaagtag tattggcgcc ttcgtttacg
     8821 gtaaggtcaa gattattgat caatgaagga ttggcattgg cagtagcagc cggatcgttc
     8881 catgtcagca ttactttcaa acgggcagct ccggcaggaa cagtaatggt tacatcattg
     8941 gaagcaccat tagctactga gtttaccaca tagcggttat cttccagtat tcttacggat
     9001 gtcaatgcat tgatacggcc aaagccgaat ttataatccg gaccaggatt tcccaggtcc
     9061 tgggcgccat tacaaataat attctttatc agtgtggatg gcggtatcgc atttccgtta
     9121 agttgtttgt aacgctggta caacaaagca gatgatccgg ccacgccggg agctgccata
     9181 gacgtacctg aaagtgtagc ataggtatta gtaggagtgc tggtagaaaa aacagcatta
     9241 cccattgcac tgatctctgg ctttatcctt ccgtcctgca caggtccgct gctgcttgat
     9301 gatgaccagg cttccgtaga agtaatatta gccacaacca ggttgttctt ggcaggttta
     9361 ccgctaccgg taattgtcag gtaaccattg ggacaaacac ctgcagaatt acccgcagag
     9421 tgtacctgca ggtggttact gttggtgttt aatacaatat cagtattcct tgccgtagtg
     9481 ttgtaagtaa gtaaaggtcc gttgatatca cagtttggac tggaagatga accataagaa
     9541 tggctgctca ccagcaggtt gttggcaggc acaccggttg tcatttccga ttgaatatct
     9601 ccgctgaaat tccaggagaa caaccttgca ttgggagcca taccccttgc agtaggattg
     9661 ataaggcctt taccggtgat tgttcctgca cagtgtgtag catgctgaat gggtgagcct
     9721 gcttctataa tggtaagacg gccggcgggt gaaaagtcga gatgctgatc taccgggcca
     9781 ccatcccaga taccgatatt aatatcatca cctttcaaat tgcggacgcc gtcattcaga
     9841 atattcaccc ggtgcaatgt tctgccgggt aagttctcta aaacattagg cggatcaatc
     9901 gcttcaaccc attgcacata aggtaagcca gccagcattt gcactgcggt ttgtggtatt
     9961 cttaagcgta atgttttgaa aacaggaaca tcggctaaaa tgcttccccc aactgactgg
    10021 atggaattca ttaccgcagc tgcgctgatt ctttcatagg tgataaccga tacatctata
    10081 taaccaaact ggttaacagc atgtggtggt atttcaccgc ggtacagagc cggatgcatt
    10141 ttttgctttt catccaactg gaatatacta cgcaatggaa aggctgataa tgcctgcaca
    10201 tttactccgc tannnnnnnn nnnnnnnnnn nnnnnnnnag tcaatcagtt cgatacctga
    10261 tgctttcagc tgatccttca atgtttgaga aggtattgtc tttagctgga tcgtaacata
    10321 atgttttccg ttaaataagc tcctcaggaa aacaggatca ctcttggtga tggtttggat
    10381 attttcctgc ggaataaatt ttccggcatg cagctgtaca ttaaagtcct gggcaaataa
    10441 atagcccggt accaacaaaa agaacagcgt ttttactgag agagtaaaaa atcgtttcat
    10501 gaaatgaagt ttaaaatatt tgccatgggg gaaatgcgga cggaaattac agaaaaatag
    10561 catctgtcca cacttttgct aaagggcttt tccctgattt gtttaggcaa acctttgtgt
    10621 gggcaaacaa ccgatttgct acctttagca tgttctaaat caacaattat ggcatttgaa
    10681 tggaaaggtg tttttcccgc cctgcttacc ccttttaatc aaaatgaaga actggacctg
    10741 cagatgtacg agaaaaacct gcaggcgcaa ttggatgcag gggtacatgg catcatcatt
    10801 ggcggctcat tgggagaggc cagcaccatt acagaaaagg agaaagaaga gctgacggag
    10861 ttttctgttg aatatatcaa tggaaaaata ccggtgatcc tcaacattgc agaaagtact
    10921 accaaagaag ctatccaaca ggcagccaat gcgaaaagat ggggagcgga tggactgatg
    10981 ctattgccac cgatgcgtta caaaaccgat gacagggaaa ccattactta ttttaaagca
    11041 gtggctcatt ctactgatct gcccattatg atttacaaca accctgtaga ttataagatt
    11101 gatacaaggc cggatatgtt tgatgaactg gttgaatgtg ccaacattac tgcattaaaa
    11161 gaaagttcaa gagatgtgac gaatctgaca aggatgaaga accgttttgg tgaccgcttt
    11221 aagattcttt gcggcgtgga tacgctggcg atggaggaac tttgtctcgg cgctgatgga
    11281 tgggtggcag gattggtgga tgcttttccg gcagaaacgg tagctattta taaattagtg
    11341 aaagccggaa aaatagagga ggctgctaaa atttacagat ggtttatgcc cgtgctggaa
    11401 ttagatttac atcccaagct agtacaatac ataaaactgg cagctatgca aaccggaatc
    11461 ggcagtgaat atgtaagagc gcctcgacta caattggaag gaacggaaag acaaaatgtt
    11521 ttacaaacta ttgatgaagc catttcaaat agaccgaaac tggtttttta atttattata
    11581 tacttatacg tgatgtttaa ggatgctaca accgatgaaa tagattctgc tctcagaaaa
    11641 gcctggcagg cgtttcattt ataccgaaaa ttttctttga agcaaagagc ggattttatg
    11701 aaggcaattg ctgttgagct tgaaaatacc ggcgacagct taatatcaac cgcaatgagg
    11761 gaaacaaatt tgccggaagc aagattgagg ggcgaaagag caagaaccat ttttcaactc
    11821 aactgttatg cagaagcctg tgtcaaggga gattggcttg aagcgagaat agatactgca
    11881 atagaaacta aaataccgcc aaagcctgat atcagaaaaa tgctggtgcc attggggcct
    11941 gttgtagtat ttggtgcttc caattttcct ttagcatatt ccactgccgg tggcgatacg
    12001 gcttgtgcat ttgcagccgg atgtccggta atcgtgaaag cacacccggc gcaccccgat
    12061 acttctgaac aggtagctca tgcaataaaa aatgcagttc aaaaatgtaa tatgcctgat
    12121 ggagtttttc agcatattca aggcaattca tttgaagtgg gtaaagcatt agtcatgcat
    12181 ccatttacaa aagcagttgg ctttaccggt tcttatactg gcggcaaaca gctttatgac
    12241 tgggcacagc aaagaaaaga acctattccc gtttttgcag aaatgggaag tattaatcct
    12301 gtttttttac taccggaaaa actaagcaaa gaagcagata cgatagc
//
LOCUS       DAORLS010000058        12210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2779_length_12210_cov_2.349074, whole genome shotgun sequence.
ACCESSION   DAORLS010000058 DAORLS010000000
VERSION     DAORLS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2779_length_12210_cov_2.349074"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK977_06315"
     CDS             <1..538
                     /locus_tag="PK977_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF17761.1"
                     /translation="KEVKLTNTELFQSINPLFIVLLTLFFVPFFSWLRKRGKEPTTMS
                     KFGMALFISGLSALVMVFAILSVPSIYTHKASAIWLWLTYFVFTISEIFLSPMGLSLV
                     SKLAPARLTALMMGGWFLSTSIGGKVAGVMTSFWDDFTDKKMFFMILVVAAFIGGILI
                     YSRIKTLNQVVKDKTGSH"
     gene            637..2268
                     /locus_tag="PK977_06320"
     CDS             637..2268
                     /locus_tag="PK977_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRF17762.1"
                     /translation="MWKKHPKALPFLFFSEMWERFGYYLMIGIFTLYLKDVKTGYAMT
                     EAESADLYGTFIALVFLTPFIGGLLADRYLGYRKSIIMGGLMMGAGYCLMSVHSLPML
                     YISMTLVILGNGFFKPNISTLLGNVYTTPQYVKQKDDGYNIFYMGINIGAFICNFFGA
                     ALYIVYGWGAAFIAAGVGMFLGVIIFLSGTRHYKAFDVKKGVGENDMSMTRIVATIFV
                     PSIAAGILGWLIPGNLVGSDSTDAFLFACLPVIYFYASLFFRATKDEKRPIAALLAIF
                     AVVVMFWAVFKQNGSALNTWADRYTNRQVTGIAQTVFEKLNFSQTITYTKDSVAKYDD
                     QFRLQKENGKIVKELNYPVYFKNVPQDKKPAEGDKVSLWATNLSQSINPGWVILLTPL
                     VVAFFTWLRRRNQEPSTATKIAFGLFISGLSALVMVGAVYASHNGTEKASVLWLISSY
                     GVITIGELLLSPMGLSLVSKLSPVRITSLMMGGWFLATSIGNKLSGVLSSMWDKYNHK
                     AGFFWVNFGLLMIAALMCFAMLKWLNKIMKEKGVS"
     gene            2375..4444
                     /locus_tag="PK977_06325"
     CDS             2375..4444
                     /locus_tag="PK977_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRF17763.1"
                     /translation="MADQQNHFKPFVAPETKMAEFTVKSILTGAAFGIIFGAATVYLA
                     LKAGLTVSASIPIAVIAITLGRKFLKTTILENNIIQTTGSAGESIAAGVVFTLPGFLF
                     LSEAGSANFFNYFTILILAIFGGVLGTLMMIPLRRSLIVKEHGTLPYPEGTACASVLK
                     AGEKGGDFAKTAFMGLGFAFGYAILQKIFHVIAETPAFMTKQTQKFFPSAKVSGEITP
                     EYMGVGYIIGPKIAGVLVAGGVLSWFVFTPLLASLLNDNGDIIAAQLVKLGYLKDVNT
                     AGGPGGWDPATHGFADWSTAIYRAYIRQIGAGAVAAGGFITLIKTIPTIISSFKGSIG
                     SLKKDGITAEANVPRTERDLSMKVVGIGSLILILVIALLPNTLIPGSNIGSKLLLGVL
                     VIVFGAFFVTVSSRIVGLIGSSNNPISGMTIATLMGTCLIFIAVKWTGHFYEPMALVV
                     GGMICIAAANAGATSQDLKTGYIVGATPKYQQLSLFIGAIVSSLAIGATIKILDQPTA
                     EMAAQGIQHAIGTDKYPAPQGTLMATLIKGILSFNLDWQFVLVGVFIAIVMELCGIKA
                     LSFAIGIYLPLSTTLPIFIGGAIRGVVEWREKRKNIKVAPEEEDLGKGNLFATGLVAG
                     GALAGVLVAFLSASDGVSKKLANVNAEHGLTSSLGGEGYKWLGVIFFALMGFVLYRIA
                     ISKRKNT"
     gene            complement(4519..5718)
                     /locus_tag="PK977_06330"
     CDS             complement(4519..5718)
                     /locus_tag="PK977_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRF17764.1"
                     /translation="MFIKRKEFIQIGSLATASLMLPKFLKAFEGKTLVPAGNKVVVIL
                     QLSGGNDGLNTVIPFRNDLYYKARPRLGIVKEKALALTGEAGLHPALTAFKELYDDGS
                     LAILNNVGYPNPDRSHFRSMDIWHTASQNNEYCNHGWVGRYLDAQCNGCDKPTQAIEI
                     DDILSLALKGENMKGIAVKDPRRLYGTANEKFFRDVMKNHTDEKGEQPVDYLYKTMAE
                     TLSSADYIFQQSRLHPTNAEYPKTDLGNSMKTIASLIFSEINTKVYYVSLGSFDTHIG
                     QEGQQQRLFTEMNDAIKAFVKDLKANGRFNDVMLFTFSEFGRRVAQNASGGTDHGTAN
                     NMFLVSGGLKQKGLINELPDLADLDEGDLKYKVDFKNVYATVLSKWLKADDKAILGKN
                     YDYLNFI"
     gene            complement(5741..7213)
                     /locus_tag="PK977_06335"
     CDS             complement(5741..7213)
                     /locus_tag="PK977_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRF17765.1"
                     /translation="MSLSNQLKNQHLMWRAGFGPAVEQLDDLSQYTPKQFYKALVKAS
                     EKKPEYINVADDYLQGLYKGLEEAGRQQRQEMNAEERKMVQQKNREGIRNLNIYWLYE
                     MVNSSAQLREKMAFFWHGHFACRNLNIFYQQGLLDVIRRNALGNFGTLLKEVSKTAAM
                     LNFLNNQQNRKDHPNENFAREVMELFTLGRGNYTEHDVKEAARAFTGWTANLRGDFVF
                     RRFQHDFGSKTVLGKTGDFDGGDVLDILLEQKQTARFVTEKIYKFFVNEQPDKSKVDW
                     LADRFYNNQYDIAKLMEDIFTSDWFFEDKNIGARIKSPIELVAGIQRMLPMKFNQEEA
                     LLLLQRALGQLLFYPPNVAGWPGGKNWIDSSTLMLRMRIPQMLNEQDEFNVKPKDDDD
                     QMMGRKDDKKDDSKEKAMKGMNRVNRPINVKIDWSLYTKHYESTPREALLTKMTVILL
                     QAKSQVNAELIKQYADQSGRENFIRTATLQLMSTPEYQLC"
     gene            7336..8394
                     /locus_tag="PK977_06340"
     CDS             7336..8394
                     /locus_tag="PK977_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HRF17766.1"
                     /translation="MRLRFLKYILPFLFFFGAWQSFHSYGWFVWLPVIAAFFIIPVLE
                     LFIKPDPSNMNAAEEEMARKSRIYDVLLYFVVAFQYFALYQFLVSMKNDTLAWYETAG
                     RIAVMGLLCGTFGINVGHELGHRVKRFEQTLAKALLLTSLYMHFFTEHNKGHHKRVAT
                     PEDPSSARYGEPVYKFYFRTIIMSYFSAWHIANDEVKKKGKAVLSLYNEMIQFQLIQV
                     AFVGTIWYFFGGLVTVYFLAAALIGILLLETVNYIEHYGLQRKKIAEGRYERALPVHS
                     WNSDHVIGRIMLFELSRHSDHHYLASRKYQVLRHHDNSPQMPTGYPGMIILSLVPPLW
                     FYVMNNRIKKNQSAGLHN"
     gene            8665..9648
                     /locus_tag="PK977_06345"
     CDS             8665..9648
                     /locus_tag="PK977_06345"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HRF17767.1"
                     /translation="MKKSLVLLAGAFLFGISCNKSITSEEQESLSPEETTSALQRNCG
                     ADEVLQQQLKADPTLRQKMNAIEDFTRQVQMNPSAYRLVNGIIEIPVVVNVLYKTTAQ
                     NISQTQIQSQIDVLNKDFSATNTDYNNTPSIFQGVRSGDIGVRFVLAGVVRKKTNKTS
                     WQLNDAMKKSSQGGIDPTSPTTTLNLWVCNLTSGYLGYAQFPGGAAATDGVVCDDNAF
                     GTTGTAAAPFNKGRTATHEVGHWMNLRHIWGDATCGNDQVGDTPVHNTANYGCPAAGH
                     TSTCSGNPVEMTMNYMDYTDDACMYMFSAGQKTRMLAIFAAGGPRNSFAQP"
     assembly_gap    9794..9875
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9876..10993)
                     /gene="serS"
                     /locus_tag="PK977_06350"
     CDS             complement(<9876..10993)
                     /gene="serS"
                     /locus_tag="PK977_06350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRF17768.1"
                     /translation="MLRQNPEWVKERLAIKHFANQGLVEEIIALDDERKKLQLESDTL
                     QSKVNAASKEIGMLMGKGQKEEAEAKKQEVAAIKASLLPLADQQTEIEKQLGEKLVLL
                     PNLPSEKVPAGKTPADNVVVREGGMKPQLSADALPHWDLIKKYDIVDFETGVKITGSG
                     FPLFKGKGAKLQRALTQYFLDFNTAAGYTEYLPPLMVNEATAYGTGQLPDKEGQMYYM
                     PADNFYMIPTAEVPVTNIYRDEIVKESDLPIKMTAFSACFRREAGSFGADVRGLNRVH
                     QFDKVEIVQLVHPEKSYEVLEEMVSHVEKLLNTLELPYRILRLCGGDMSFASALTYDF
                     EVFSAAQQKWLEVSSVSNFEAFQTNRMKIRFKDEKGKS"
     gene            11101..11445
                     /locus_tag="PK977_06355"
     CDS             11101..11445
                     /locus_tag="PK977_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17769.1"
                     /translation="MLPADDLQQRWWNLEAKLVERFGKKPDMEAILFLIGIQEFGEIR
                     EKFTKEQKQDLMHVAVCNLLSQSGYYELESVDADGWPHFRQLKPMPDMSMPEQENFLK
                     DHILLYFEQHNF"
     gene            11448..12143
                     /locus_tag="PK977_06360"
     CDS             11448..12143
                     /locus_tag="PK977_06360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF17770.1"
                     /translation="MKKLILLAFTAVFFTYSEAQSDSTLKKKDRKRDVLIQTSMGDMT
                     VRLSDSTPLHRDNFLKLVKVHFYDSLLFHRVIKDFMIQGGDPNSRNAKPGEALGMGGP
                     GYRVPAEFRQTLFHKKGVLAAARDNNPEKASSGCQFYIVQGKTFTDAELDNLETSRLK
                     RKIPTEHREVYKVLGGTPHLDQNYTVFGEVIKGIEVLDKIAAVETSKGADRDRPVQDV
                     KIITMKLVKRKKK"
BASE COUNT         3258 a         2632 c         2572 g         3666 t
ORIGIN      
        1 taaagaagta aaactgacca atacagaact atttcaatcc atcaaccctt tatttattgt
       61 attgctgact ttattctttg tacccttttt cagttggctc cgcaaacggg gcaaagagcc
      121 aacgacgatg tcgaaattcg gaatggcctt gttcatctcc ggcctgtcgg ccctggtaat
      181 ggttttcgcc atcctgtcgg ttccttctat ttatacccat aaggcatcag ccatttggtt
      241 atggcttact tattttgttt tcaccatcag cgaaattttc ctgagcccga tgggattgtc
      301 attggtatca aaactggcgc ctgcccgttt gacagcgctg atgatgggcg gctggttctt
      361 atccacttct attggtggta aagttgccgg tgtaatgaca agcttctggg atgattttac
      421 tgacaagaaa atgttcttca tgatactggt tgtggcagcc tttattggag gtatcctgat
      481 ctattcaaga atcaagacgc tgaaccaggt tgtaaaagac aaaacaggtt ctcattaatc
      541 aaattttgct ctataaaaaa aggcggccaa caaagccgtc ttttttattt tcactaacct
      601 catttttggt atcttgacca cttaaacgat tgagttatgt ggaagaaaca cccgaaagcc
      661 ctgccctttt tatttttctc tgaaatgtgg gagcgatttg gttattacct gatgatcggc
      721 atttttaccc tgtacctgaa agatgtgaaa acgggttatg ccatgacgga agcagagtca
      781 gctgaccttt acggaacctt tattgccctg gtattcctta ctccttttat cggtggcctg
      841 ctggccgacc gctacctggg ctaccgcaaa tccatcatca tgggaggcct gatgatgggc
      901 gcaggctatt gtcttatgtc ggtgcatagt cttccgatgc tttatatttc catgacgctg
      961 gttattcttg gtaatggttt ttttaaacca aatatttcca ccctgctggg caatgtttat
     1021 accacaccgc aatatgtaaa acaaaaagac gacggttata atatctttta catgggtatt
     1081 aatatcggcg ctttcatctg caatttcttt ggcgctgcgt tatacattgt ttatggctgg
     1141 ggtgctgcat ttattgcggc cggggttgga atgttcctgg gtgtaattat ttttctttcc
     1201 ggcacccggc attacaaagc atttgatgta aagaagggag taggagaaaa tgatatgtca
     1261 atgacccgta ttgtggcaac catttttgta ccctccatag ctgcaggtat cctcggatgg
     1321 ctgatacccg gcaacctggt aggctccgac tccactgatg cgttcctttt tgcctgtctg
     1381 cctgttatct acttttatgc ttcgctgttt ttcagagcaa ccaaagatga gaaaagacct
     1441 attgcagctc tgctcgccat ttttgccgta gtggtaatgt tctgggctgt attcaaacaa
     1501 aatggttcgg cgctgaatac ctgggcggac cggtatacta accggcaggt aactggcata
     1561 gcacagacag tatttgagaa gctgaatttt tcacaaacca ttacttatac aaaagattct
     1621 gtagcaaaat acgatgacca gttccgtttg caaaaagaga acggtaagat tgtgaaagaa
     1681 cttaactacc cggtttattt taaaaatgtt cctcaagata aaaagccggc agaaggtgat
     1741 aaagtgagtt tgtgggctac caacctgagc cagtcaatca atccgggctg ggtgatattg
     1801 cttactccgc tggtagttgc cttctttacc tggctgcgca gaaggaacca ggaaccttct
     1861 acagccacca aaatcgcatt tggacttttt atctccggct tatccgcact ggtgatggtg
     1921 ggtgcggtat atgccagtca taacggaaca gaaaaagcca gtgtgctttg gctgatatcc
     1981 agttatggtg tgataacaat cggcgagctt ttgctaagcc cgatgggact atcactggta
     2041 tccaaattaa gccctgttcg cattacgtca ttgatgatgg gtggatggtt ccttgctact
     2101 tccatcggta ataaattatc cggagttctt tcctctatgt gggacaaata caatcacaag
     2161 gcaggtttct tctgggttaa ttttggttta ctgatgattg ctgccctcat gtgttttgca
     2221 atgctgaaat ggctgaataa gataatgaaa gaaaaaggag ttagttgagt cgataataat
     2281 gtgtaattag taaattcaat aggaatgttt ttggctattg actaatgact attgactgtt
     2341 ttccctatct tccctataac aaaacctaac tgccatggca gatcagcaaa atcatttcaa
     2401 gccatttgtt gcccctgaaa caaaaatggc ggaattcacc gtaaagtcca ttcttaccgg
     2461 tgctgcattc gggataattt tcggtgcggc cacggtttac ctggccttga aggcaggcct
     2521 tactgtttca gcttccatac ccatcgccgt aattgcgatt acactggggc gaaagttttt
     2581 aaaaacgacc attcttgaaa ataatatcat tcagacaacc ggttctgccg gagaaagtat
     2641 tgcagccggg gttgtattca ccctgccggg atttcttttt ttgagtgagg caggcagcgc
     2701 caacttcttc aactatttca ccatattgat actggccatc ttcgggggtg tactgggcac
     2761 cctgatgatg atacccttgc gtcgttcact tattgtgaag gaacatggca cattgcctta
     2821 cccggaagga accgcctgtg cctctgtttt aaaggcaggt gaaaaaggag gtgactttgc
     2881 caaaacagct tttatgggtc tgggctttgc atttggttat gccattttgc aaaagatatt
     2941 tcatgtaatt gccgaaacac cggcgttcat gaccaaacaa acacaaaaat tctttccatc
     3001 cgcaaaagtg agcggagaga tcacacctga gtatatgggt gtgggctata tcatcgggcc
     3061 aaagattgcc ggtgtactgg tggccggtgg tgtgttaagc tggtttgttt ttaccccttt
     3121 gctggccagt ttactgaatg ataatggtga tatcattgcg gcgcaattag taaagcttgg
     3181 atatttaaaa gatgtgaata ccgccggtgg acccgggggc tgggatcctg ccactcatgg
     3241 ttttgctgac tggtcaaccg ctatttaccg ggcttatatc cggcagatag gtgccggcgc
     3301 tgtagcagca ggtggtttta tcacattgat caaaacgata ccaacgatca tctcttcttt
     3361 taaaggcagt attggttcat tgaaaaaaga tggcattact gcagaagcca atgtgcccag
     3421 aacggaaaga gatctgtcaa tgaaagttgt aggcattggc agtttaatac tcattcttgt
     3481 tattgcatta ctacccaata cactgatacc cggaagcaat atcggcagca aattattgct
     3541 cggtgtgctt gtaattgtgt ttggtgcatt tttcgtaacc gtttcttcca gaatcgttgg
     3601 tctcatcggt tcatccaata atcctatcag cggtatgacc atcgctaccc tgatgggaac
     3661 ctgtttaatt ttcatcgctg ttaaatggac cgggcatttt tacgaaccaa tggcactggt
     3721 agtaggcggt atgatctgta ttgctgccgc taatgcagga gccacctcac aagacctgaa
     3781 aacaggttat atcgtaggcg ccactccgaa atatcagcag ctgtcattgt tcatcggtgc
     3841 catcgtttca tctttggcaa tcggcgctac gattaaaatt ctggatcagc caaccgctga
     3901 aatggcggca cagggtatcc agcatgctat cggaacagat aaatatcccg caccacaggg
     3961 aactttaatg gcaacactaa ttaaaggtat tctgtcattc aaccttgact ggcagtttgt
     4021 gctggtgggt gtattcattg ctattgtaat ggaactttgt ggtataaaag cattgagttt
     4081 tgcaattggt atttaccttc cgctgtctac tacgttgcca attttcattg gtggcgctat
     4141 ccgcggtgtt gttgagtgga gagaaaaaag aaagaatatt aaagtggcac cggaggaaga
     4201 agatcttggc aaaggaaatc tttttgcaac tggcctggtg gcgggtggtg cgttagccgg
     4261 tgtattggtt gctttccttt ccgccagtga cggcgtgagt aaaaaattgg cgaacgtaaa
     4321 tgcagagcat ggccttacca gttcgctggg aggtgaaggt tacaaatggc tcggggttat
     4381 tttctttgcg ttaatgggat ttgtgttgta ccgcatcgcc ataagcaaga gaaagaatac
     4441 gtaaaacaat actgatgtaa aaagcccccg ccggtaccgg caggggcttt tagtttccgg
     4501 ttacggatct gtaacagctt aaataaaatt caggtagtcg taattttttc caagtattgc
     4561 tttgtcatca gccttcagcc atttgctgag tacggtagca tatacatttt tgaaatccac
     4621 tttatacttc agatcacctt catcaaggtc agccaggtcg ggtagttcat tgatgaggcc
     4681 tttttgtttt aatccgccac tgaccaggaa catattattg gccgtaccat ggtcggtacc
     4741 accactggca ttttgtgcca cccttcggcc aaattcagaa aaagtaaaca acatgacatc
     4801 attaaaacga ccattcgctt tcaggtcttt cacaaaggct tttatcgcat cattcatttc
     4861 tgtgaacagt ctttgttgct gaccctcctg tcctatatgt gtgtcaaaac ttcctaatga
     4921 cacataatag accttcgtgt tgatctcaga gaagatcagc gatgcaatcg tcttcatact
     4981 attgccgaga tccgttttgg ggtattcggc attggtagga tgcaaccggc tttgctgaaa
     5041 aatataatcg gcacttgaca atgtttctgc cattgtttta tacaaataat caaccggctg
     5101 ttcgcctttt tcatccgtgt gatttttcat cacatccctg aaaaattttt cattggcagt
     5161 accatacaaa cgacgaggat cttttactgc aatgcctttc atgttttctc cttttaaagc
     5221 tagacttagt atatcatcta tttcaatggc ctgggtcggt ttgtcgcatc cattacattg
     5281 tgcatccagg tagcgtccaa cccaaccgtg gttacaatat tcattgtttt ggctggctgt
     5341 atgccagata tccatacttc tgaaatgtga acgatcaggg ttcggatatc ccacattatt
     5401 taatatcgca aggctgccat catcatataa ttctttgaat gcagtaaggg ccgggtgaag
     5461 acccgcttca cccgtcaagg ccagcgcttt ctcttttaca attcctaatc ttggcctggc
     5521 tttatagtac aggtcattcc tgaaaggaat caccgtattc aaaccatcat taccgccact
     5581 gagttgcaaa atcaccacta ctttattgcc tgcaggcacc agcgtctttc cttcaaaagc
     5641 tttcaagaat ttggggagca tcagcgatgc agtggccaat gaaccgatct gtatgaattc
     5701 tttacgtttt ataaacataa ttgtatcgtt tttgaaccgg ttagcacaat tgatattccg
     5761 gtgtactcat taattgcaga gttgctgttc ttataaaatt ctcacggccg ctttgatcag
     5821 catattgctt aatcaattca gcattgacct ggctttttgc ctgcaacaat ataactgtca
     5881 tttttgtcag cagggcttcc ctgggcgtag attcataatg tttggtatat aatgaccagt
     5941 caatcttcac attgatcggc cggttcaccc ggttcattcc tttcatagcc ttttcctttg
     6001 agtcatcctt tttatcatcc tttctgccca tcatctggtc atcatcatcc tttggtttta
     6061 cattgaattc atcctgttca ttcagcatct gcggaatccg catccttaac atcagtgtgg
     6121 aactgtcaat ccagttttta ccaccgggcc aaccggccac attgggcggg tagaacaata
     6181 actgacccaa tgccctttgc agcagcagca atgcttcttc ctggttaaac ttcatgggaa
     6241 gcatccgttg tatacccgct actaattcaa tgggcgattt tattctggca ccaatattct
     6301 tatcttcaaa aaaccagtca ctggtgaaaa tatcttccat cagtttcgct atatcatact
     6361 ggttgttata aaatctatct gccagccaat ccacttttga tttgtctggc tgttcattca
     6421 caaagaactt atatattttt tctgtaacga accgggctgt ttgcttttgt tccagtaata
     6481 tatccagcac atctccgcca tcgaagtcac cggttttacc cagcacggtt ttgcttccga
     6541 agtcatgctg aaagcggcga aaaacaaagt cgcccctcag gttggcagtc cacccggtaa
     6601 aagccctagc tgcttctttt acatcatgct ccgtataatt tccacgaccc agtgtaaaca
     6661 attccattac ttcccgggca aaattttcat tcggatgatc ttttcggttt tgctggttat
     6721 tcagaaaatt cagcatggct gctgttttgg aaacttcttt cagcagggtt ccaaaattgc
     6781 ccagggcatt tcgccggatc acatccagca aaccctgctg gtaaaagata ttgaggttgc
     6841 ggcaggcaaa atgcccatgc cagaagaaag ccattttttc cctgagctgt gcagagctgt
     6901 tcaccatttc atacagccag tagatattca gattgcggat accctcccgg ttcttttgct
     6961 gtaccatttt tcgttcttcg gcattcattt cctgtctttg ctgacggccg gcttcttcca
     7021 gtcctttgta cagaccctgc aggtagtcat ctgctacatt gatgtactcc ggtttctttt
     7081 cggatgcttt taccagggct ttataaaatt gcttcggggt atattgggac aaatcgtcca
     7141 actgctcaac agcgggacca aaaccggccc tccacatgag gtgctggttt ttcaactggt
     7201 tcgacaatga catcaggcta acatttaggt ttcagactta aaagcattct tcaggtttaa
     7261 caaccaagtt attaccagac gtaaaacgtc ataatacctt tcttattttt tacatttgat
     7321 ctgtataaat tttatatgcg gttacgattc ctgaagtaca tccttccttt tcttttcttt
     7381 tttggcgcct ggcaatcatt ccacagctat ggatggtttg tttggctgcc ggtgatagca
     7441 gcttttttca ttatcccggt actggaactc ttcattaaac cagacccttc caatatgaat
     7501 gctgcagaag aagagatggc aagaaaaagc aggatatatg acgttctttt atactttgtg
     7561 gttgccttcc aatatttcgc tttataccaa tttttggtgt ccatgaagaa tgacaccctt
     7621 gcctggtatg aaactgccgg aagaatagct gtgatgggcc tgttatgcgg aactttcggt
     7681 attaatgtag gacatgaact tggtcaccgg gttaagcggt ttgaacaaac actggccaaa
     7741 gcccttttac ttacctctct ttacatgcac ttttttacgg aacataataa aggccatcac
     7801 aaaagagtgg ctacaccgga agatcccagc agtgcccggt atggagagcc agtctataaa
     7861 ttttatttcc gtaccatcat catgagctat ttcagtgcct ggcatatcgc caatgatgaa
     7921 gtgaagaaaa aaggaaaagc cgttttgtct ttgtacaacg aaatgataca gttccagcta
     7981 atacaagttg cttttgttgg gacgatctgg tatttttttg gcggcctggt aacagtgtat
     8041 ttcctggcag cagcactaat tggtatattg ttactggaaa ccgttaatta tattgagcat
     8101 tacgggctgc aacgaaaaaa aattgctgaa ggaaggtatg aaagggcttt gccggtgcat
     8161 agctggaaca gcgaccatgt aatcgggcga ataatgttgt ttgaactgag ccgtcattct
     8221 gatcatcatt atcttgccag cagaaagtac caggtacttc gtcaccatga taactctccg
     8281 caaatgccaa ccggttatcc gggaatgatc attttatcac tggtgccgcc gctttggttt
     8341 tatgtgatga ataatcgcat caagaaaaat cagtctgctg gtttgcacaa ttagtaaata
     8401 cctgaaacga taagtaaatt ttttatgctg ctttaacctt tattctatga cagcataaca
     8461 aaagcattat gggttagcca atagtaattc catcataaca ttatttagca accaacgtaa
     8521 attttctata ccagttgcag gttattgccg gttcgacacc ttgttgcaat aatagtttac
     8581 ccagacttat caaataatga aaaatgctga taaatttaaa agtcattttt tatttcacac
     8641 ctaaaacact caaattctaa ttgtatgaag aaatcactcg tattgcttgc cggtgcattt
     8701 ttgtttggca tcagctgtaa caaatcaatt acctctgaag aacaagagtc cctcagccct
     8761 gaagaaacta catctgccct gcaaagaaat tgcggtgctg atgaagtatt gcagcaacaa
     8821 ctaaaagccg atcctacatt aaggcaaaaa atgaatgcta ttgaagactt tacccgccag
     8881 gttcagatga acccttctgc ttacaggctt gtgaatggta ttattgaaat acccgttgtt
     8941 gtaaatgtgc tttataagac aacagcacaa aatatttcac agactcagat acaatcacaa
     9001 atagatgtac tgaataagga ttttagtgca acaaatacag attacaataa cactccctcc
     9061 atattccagg gtgttagatc gggcgacatt ggtgttcgtt ttgtgttggc cggtgtggtt
     9121 cgtaaaaaaa ccaacaaaac cagctggcaa ctgaatgatg ccatgaaaaa aagttcacaa
     9181 ggaggtattg atcctaccag ccctaccact actcttaatc tttgggtgtg taacctgaca
     9241 agcggttatc ttggttatgc ccagttcccg ggtggtgctg ctgccacaga tggagtagtt
     9301 tgtgatgata atgcatttgg tacaaccggt acagcggctg cgccttttaa caaaggaaga
     9361 acagctactc atgaagtagg tcactggatg aacctgcgtc atatctgggg tgatgcgacc
     9421 tgcggaaacg accaggtagg cgatactcct gttcacaata cggccaacta tggatgtcct
     9481 gcagccggtc atacaagtac ttgttctggc aatcctgttg aaatgaccat gaattatatg
     9541 gactataccg atgatgcttg tatgtatatg ttcagtgccg gtcaaaaaac gaggatgctg
     9601 gccatttttg ccgctggtgg tcccaggaat agttttgcac aaccataata aaagactgaa
     9661 cactttaaaa tacaaaaccc cgcaatgcgg ggttttgtat tttaaatagc attgaaataa
     9721 attgaaccca ctaagaaatt actttccctc caaaataaga atgtaatact gcaggcaaac
     9781 tgattccttc ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgtga tttacctttt tcatccttaa
     9901 agcgaatctt catacggttg gtctggaatg cctcaaagtt ggaaacagaa cttacctcca
     9961 accatttttg ctgtgccgca ctgaacactt caaagtcata tgtaagcgca gaagcaaaac
    10021 tcatatcgcc gccgcaaaga cgaagtattc tgtaaggtaa ttctaaagtg ttcagcagtt
    10081 tttctacatg gctcaccatc tcttccagca cttcataact tttttccgga tgaaccaatt
    10141 gcacaatttc cactttatca aactggtgaa cacggttcaa ccctcgtaca tcggcgccaa
    10201 agctacctgc ctctcttctg aaacaagctg agaaagccgt cattttgatt ggtagatctg
    10261 attcctttac aatttcatcc ctgtaaatat tggtaacagg cacttccgca gttggaatca
    10321 tgtaaaaatt atcagcaggc atataataca tctggccttc tttatcaggt aactgaccag
    10381 tgccataagc agtagcctca ttcaccatta gcggtggcag gtactctgta taacccgctg
    10441 cagtattgaa atcaagaaaa tactgggtca atgcccgctg tagcttagcc cctttacctt
    10501 taaataaagg gaaaccgcta ccggttatct tgacccccgt ttcaaaatca acgatatcat
    10561 attttttgat caggtcccag tgtggaagag cgtcagcaga caattgtggt ttcatccctc
    10621 cttctcttac caccacatta tccgcaggcg tttttccggc cggcactttt tcggatggca
    10681 ggttgggcaa taataccagc ttttcgccca gttgtttttc aatttcagtt tgctgatcag
    10741 ccaatggcag taaagatgct tttattgccg ccacctcctg tttctttgct tcagcttctt
    10801 ctttttggcc tttgcccatc agcattccga tctccttcga tgctgcattt acttttgact
    10861 gcaacgtatc cgactctaat tgtaattttt ttctttcatc atctaacgca atgatctctt
    10921 ctaccagtcc ctgattggca aaatgtttta ttgccaatct ttcctttacc cattcgggat
    10981 tttgacgtaa cacctgtacc tgcaacattt tagttcaatt tgcggcaaag ataattgctg
    11041 aggggcatgc ataaaccgat ttcttaaaca gccaattatg acgggttata tatttgcagg
    11101 atgttacctg ctgatgattt acaacaacgc tggtggaacc tggaagccaa attggtggag
    11161 cgattcggga agaaaccgga tatggaagcc attttattcc tgatcggtat ccaggaattt
    11221 ggcgagataa gagaaaaatt taccaaggaa cagaaacagg acctgatgca tgtggctgta
    11281 tgcaacctgc tgtcgcaaag cggctactat gaactggaaa gtgttgatgc tgatggctgg
    11341 ccgcatttca ggcagctaaa gcccatgcct gatatgagta tgccagaaca agaaaacttt
    11401 ttaaaggatc atatattact ttatttcgaa cagcataatt tttaatgatg aagaaattaa
    11461 tcttattggc atttactgct gtcttcttta cttacagcga ggcgcaatca gactcaacac
    11521 taaaaaagaa agaccgcaaa agagatgtat tgattcagac atcgatgggt gatatgacgg
    11581 tccggttgtc ggactctacc ccgcttcacc gggataattt tttaaagctg gtaaaagttc
    11641 atttttatga cagccttttg tttcaccggg tgataaaaga ttttatgata cagggtggcg
    11701 atcctaacag ccgcaatgcc aaacccggcg aagcattagg aatgggtggc cctggttacc
    11761 gggttcctgc agaattccgg caaacgctgt ttcacaagaa aggtgtgctt gctgccgcaa
    11821 gggataataa ccctgaaaaa gccagcagcg gctgccagtt ttatattgtg cagggaaaaa
    11881 catttacaga tgccgagttg gacaatttgg aaacatcaag attaaaaaga aaaataccta
    11941 cagagcatag ggaagtttat aaagtacttg gcggcacacc tcatctcgat caaaattata
    12001 cagtattcgg agaggtgata aagggcatag aagtacttga taagattgca gctgtagaaa
    12061 ccagtaaagg agccgacaga gaccggcctg tacaggatgt aaaaattatt accatgaagc
    12121 tggtgaaaag aaagaaaaaa taaaagagtc ccgggttacc gggactcttt ttatatgtta
    12181 tagtttgata aacttttatt gacctattcc
//
LOCUS       DAORLS010000059        12131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2800_length_12131_cov_2.146323, whole genome shotgun sequence.
ACCESSION   DAORLS010000059 DAORLS010000000
VERSION     DAORLS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2800_length_12131_cov_2.146323"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1312
                     /locus_tag="PK977_06365"
     CDS             92..1312
                     /locus_tag="PK977_06365"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRF17771.1"
                     /translation="MKSFDVPIIYRSPLISAVKKMRKEMDKMKKDFTPTLLDFGPLQV
                     YLARHFGFCYGVENAIEIAFRTVEKNPGKRIFLLSEMIHNPQVNADLIAHGIEFLQDT
                     HGKQLIPFEQITADDIVLIPAFGTTLEIEEKLRKIGIQTEKYNTTCPFVEKVWNRSEQ
                     IAGKDYTIIIHGKPQHEETRATFSHAAANAPSVVIKDMKEAIELGRFISGERPAEEFY
                     TVFRNQYSEGFDVNNDLQRIGVVNQTTMLASDTQSIADYLKKIMLETYQPANAEERFA
                     ETRDTLCYATNDNQTAVTGMLQTKADLAIVVGGYNSSNTSHLVELCEERLPTYFINNE
                     EKILSEQEILHFNFHTKEELQTSNYLPGKSTVKILITSGASCPDALVEGVIRKLVSFY
                     PEAKSMDEIISSFQ"
     gene            complement(1356..3011)
                     /gene="glgP"
                     /locus_tag="PK977_06370"
     CDS             complement(1356..3011)
                     /gene="glgP"
                     /locus_tag="PK977_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRF17772.1"
                     /translation="MSFTPFSVPYPVEERYSKKVAYFSMEFATHQPLKIYSGGLGFLA
                     GSHLRSAYELRQNLIGIGILWKYGYYDQERNQDQTLEVTWNEKLYSFLEDTGIKFQIT
                     IHEHPVWVKAWYLNPDTFKTAPLFLLSTDLPENDYVSQTITHRLYDANVATKVAQFIL
                     LGVGGAKLIDLLGFNPELYHLNEAHGLSAAFYLYKKFGNNLSDVKKRLVFTTHTPEEA
                     GNEKHDIYLCHKMSYFCGLSVDEVKNLYGNDSDQFNHSLAALRFAKLANGVSQLHGHV
                     SRAMWGKHENICPIISITNAQNWRYWADKQLYKFMEAGDDYGIDDRKKYLKKRAFEIV
                     ADQTGKLFSPDVFTIVWARRFAGYKRAGLITTDEHRFEKLLSNSKYPVQIIWAGKPYP
                     VDHPAISEFNQLVHLSKRYKNVAVLIAYELGLSKRLKQAADVWLNNPRVPREASGTSG
                     MTAAMNGAINFSTDDGWIPEFINHGHNGFVVPKADYANMAVYEQDEYDLEKLYEILEK
                     EILPLYYENYNTWRQVMKNGMQDVRFQFDSNRMAHEYYELLYK"
     gene            complement(3154..4131)
                     /locus_tag="PK977_06375"
     CDS             complement(3154..4131)
                     /locus_tag="PK977_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17773.1"
                     /translation="MRLFLKFLFLICLVIGLGSCKGKKKISLSGEDPVEVSDFIEFFQ
                     PLNLPVQFSDSSLAKKEKDSLLISYKIFTQFVPDSVLRKVFAKGVKPKIYALGKAVVP
                     KAEQYLFVKTVSADKRAYFLLAFDEKEQFIAGMPLLRPDKQSSTSQSAILDRKYTITQ
                     TMARKNRDGTISEGKEVYVLNAAAKNFMLIMTEALEDKITELINPIDTLPRKHKWSAD
                     YANGKMNLVSVRDGRKNDRISFFIHFEKDNGACTGELKGEALIKSSNTAEYKEEGDPC
                     RLKFIFSSNAVTLKEEEGCGSRRGLKCSFDGSFARKKYVKPSAGSKQKR"
     gene            4232..4894
                     /locus_tag="PK977_06380"
     CDS             4232..4894
                     /locus_tag="PK977_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRF17774.1"
                     /translation="MLNYSVSEENYIKAIFHLQQQDGTVTTNELAGELNTRPASVTDM
                     MKKLKAKKLVHYQPYRGFRLSAEGSKVALGIIRRHRLWEYFLAEKLKFSWDEVHAVAE
                     ELEHVSSKKLIEKLDEFLGYPKADPHGDPIPDVNGKIEHYSYQCLASLPANKLVVVSH
                     VSNQSNEILDLLKHKKISIGTKLEIKRRFDFDDSMEIKIGRQPVFTISKQVAENIFVK
                     IN"
     gene            4896..>5444
                     /locus_tag="PK977_06385"
     CDS             4896..>5444
                     /locus_tag="PK977_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRF17775.1"
                     /translation="MEALEKFLVEIGPVWSALIATTFTWLVTAVGASFVFFFKTLSRG
                     VLDPMLGFTGGVMVAASFWSLLNPAIEMSEKMYPGLSWMPASVGFLLGALFIFVLDKF
                     TPHLHINFGVEESEGVKTKLHKTTLLILAITLHNIPEGLAVGVLFGAAAIGMEDASVS
                     GAIALAIGIGIQNFPEGIAVAMP"
     assembly_gap    5445..5643
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5733..6335)
                     /locus_tag="PK977_06390"
     CDS             complement(5733..6335)
                     /locus_tag="PK977_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF17776.1"
                     /translation="MESLQLLKDYLRKFIDLPDDEFNSVLLPFIKIRKFDKRELLTKA
                     GETENYFNFILKGLIRKYYKKGRDEINTQISFEGHIIHSQESFHSRKPSEYFVEAIES
                     SVVASITYDDLESVFARTQKMEHMGRLVITHTMILKDRWQMQLVKMTPRERFINFVTR
                     NPELMQRVPQKYLASYLNIKPETFSRFKHLLRNHHRSSTV"
     gene            6507..7742
                     /locus_tag="PK977_06395"
     CDS             6507..7742
                     /locus_tag="PK977_06395"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HRF17777.1"
                     /translation="MSQKIKVANPVVELDGDEMTRIIWKFIKDKLILPYLELDIKYFD
                     LGIEYRDKTDDQVTVDAANAIKQYGVGIKCATITPDEARVKEFNLKQMWKSPNGTIRN
                     ILDGTVFREPIVINNVPRLVTNWTAPIIVGRHAFGDQYRATDTVIKGKGKLTMTFTPE
                     GGGEPQTFEVFNFKGDGVAMTMYNTDESIKGFARSCFNMALSKKWPLYLSTKNTILKK
                     YDGRFKDIFEDIYQNEFKAQFDAAGITYEHRLIDDMVASALKWNGNFVWACKNYDGDV
                     QSDTVAQGFGSLGLMTSVLITPDGKTMEAEAAHGTVTRHYRDHQAGKPTSTNPIASIF
                     AWTRGLAFRGKLDGNQPLIDFSNALEAVCIETVEQGKMTKDLAVCIHGNKVNHGEHYL
                     YTEEFLEAIDENLKKKLGK"
     gene            complement(7896..8021)
                     /locus_tag="PK977_06400"
     CDS             complement(7896..8021)
                     /locus_tag="PK977_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17778.1"
                     /translation="MADKLANEIVAASKGEGAAFKKKEDTHRMAEANKAFAHFKV"
     assembly_gap    8022..8091
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8092..8360)
                     /locus_tag="PK977_06405"
     CDS             complement(<8092..8360)
                     /locus_tag="PK977_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRF17779.1"
                     /translation="MRKAQAKKLPLAPDPKFNDKLVTRFVNNLMWEGKKSGAFTIFYD
                     ALDKVAKFTNEDGYEVWKKALANVTPAVEVRSRRIGGATFQIPSEV"
     gene            complement(8442..>8546)
                     /locus_tag="PK977_06410"
     CDS             complement(8442..>8546)
                     /locus_tag="PK977_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRF17780.1"
                     /translation="VRYHIVRGSLDTAGVKDRKKSRSKYGTKKEKAKK"
     assembly_gap    8547..8584
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8585..8820)
                     /gene="rpsL"
                     /locus_tag="PK977_06415"
                     /pseudo
     CDS             complement(8585..8820)
                     /gene="rpsL"
                     /locus_tag="PK977_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
     assembly_gap    9069..9098
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9099..10030)
                     /locus_tag="PK977_06420"
     CDS             complement(<9099..10030)
                     /locus_tag="PK977_06420"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRF17781.1"
                     /translation="MDISIRKAERSKLQDLNLENLPFGKFFSDHMLEADYENGEWKNI
                     EIKPYQPLLLSPSLAALHYGQAIFEGIKAYKDKNGEAFIFRPYDNFKRFNISAERMAM
                     PAVPEELFMDGMLQLVGLDRNWIPTQQDHSMYIRPFMFSSDEMIGVRPSENYKFMIIL
                     SPSGPYYSAPMRIYVEEKYVRAVTGGVGYAKAAGNYGAAMYATAEAKKKGYDQVLWTD
                     PFEHKYVQECGTMNVFFIIGNTAITPSLEPGTILPGVTRLSAITLLQEMGFTVEEKNI
                     SIDDLIDAYKAGIFYEAFGTGTAATISFIKELRY"
     gene            10115..10654
                     /locus_tag="PK977_06425"
     CDS             10115..10654
                     /locus_tag="PK977_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17782.1"
                     /translation="MDLNHIKLPATVINELYKTVLVEPVQNTGMKEAPAPAEKPVITS
                     AEATTPEWKSLGSNEKNILIVVSNSDAVYLPDNDLNFLTGVLGACKLSLADVAVVNRF
                     HYPEAGYKELIAYFKSRVILLLGVEPAAMGLPINFPHYQLQAFQNNTFLFTPPLEEIE
                     KDKLEKSKLWVSLKRLFNV"
     gene            10659..11246
                     /gene="rdgB"
                     /locus_tag="PK977_06430"
     CDS             10659..11246
                     /gene="rdgB"
                     /locus_tag="PK977_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRF17783.1"
                     /translation="MKLIFATNNQHKVDEIRNVLGQTLDIITLKEAGIEADIPEPYDT
                     LEANASGKSAYIFQLTGADCFSEDTGLEVAALNGEPGVKSARYAGEHRSFDANIDKLL
                     SNLAAKQDRSARFRTVISLLIDGKEMLFEGICNGQIITEKRGELGFGYDPVFIPEGSD
                     RTFAEMSLEEKNRFSHRKKATEKLVAFLNKLSTNH"
     gene            11249..>11748
                     /locus_tag="PK977_06435"
     CDS             11249..>11748
                     /locus_tag="PK977_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF17784.1"
                     /translation="MTPERNQIIPESDLINGCLQGNRRMQEELYRRFSPRMYAVCLRY
                     AGNAEEAEDILQEGFIKVFKKLDSFRGDGSFEGWIRRIFVNTAIEHFRRKRYLMPVTE
                     KEENTIEGKYTSVLDELGAKDIMALIQELSPGYRTVFNMYVVEGYTHKEIADMLGISE
                     GTSKSQL"
     assembly_gap    11749..11917
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11918..>12131
                     /locus_tag="PK977_06440"
     CDS             <11918..>12131
                     /locus_tag="PK977_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17785.1"
                     /translation="DSYALQHHFPKKLQTVEIAPPAYCWNNIAATLDGKGAEAALAAK
                     LHKASVVPPAAVWQNIEAALDEETNLP"
BASE COUNT         3446 a         2455 c         2428 g         3296 t
ORIGIN      
        1 catacaccgg tctttttgcg aaagataaga agaacatttg atgaagatgg ttgttgttaa
       61 tcacacaatc aagtattatt acctttacac catgaagagt tttgacgttc ctatcatcta
      121 caggagtcca ttgatttcag ccgtaaaaaa aatgcggaaa gaaatggata aaatgaaaaa
      181 agactttaca ccgacccttt tggattttgg cccgctgcag gtatacctgg ctcgtcattt
      241 cggattttgt tatggagttg agaatgcaat agaaattgct ttcagaactg tggaaaaaaa
      301 tcccggcaaa cgtatcttcc tcctaagcga aatgattcac aacccgcagg taaatgctga
      361 tcttattgca catggcattg aatttttaca ggatactcat ggtaaacaac taataccatt
      421 tgagcaaata acggctgatg atattgtact gatacctgct ttcggcacta ctcttgaaat
      481 tgaagaaaag ctcagaaaaa ttggtatcca gactgaaaaa tacaatacca cctgcccctt
      541 cgtagaaaag gtatggaaca ggagcgaaca gatcgcaggg aaggactata ccatcatcat
      601 tcatggcaaa ccgcaacatg aagaaacaag ggctacattc tcccatgcgg ctgccaatgc
      661 cccttcagtt gtaattaagg acatgaagga agctatagaa cttggcagat ttatctccgg
      721 cgaaagacct gcagaagaat tttatactgt tttcagaaac caatattctg aaggttttga
      781 tgtaaacaat gacctgcaaa gaatcggtgt agtcaatcaa actacgatgc tggccagcga
      841 tacgcagtct atcgctgatt atttgaaaaa aattatgctg gaaacgtacc agccggcaaa
      901 tgcggaagaa agatttgcgg aaacaaggga tacactatgt tatgccacta atgataacca
      961 gacggcggtg acaggtatgc tgcaaacaaa agccgacctg gctattgtcg tcggtggcta
     1021 caattcttcc aacacctcgc acctggtaga gctttgtgaa gaaagattac cgacttactt
     1081 tatcaataat gaagaaaaga ttttaagtga acaggaaata ttgcatttca actttcacac
     1141 caaagaagaa ttacaaacca gtaattacct gccaggtaaa tcaactgtaa aaatcctgat
     1201 caccagcggt gcttcctgcc ccgatgcgct ggtagaggga gtgatacgca aactggttag
     1261 cttttatccc gaagcaaaat caatggatga aatcatcagc agctttcaat aaaaaatccc
     1321 ggcggtgacc gggattaaat tacgttgact atctgttatt tataaagcag ttcatagtat
     1381 tcatgtgcca tccggttgct gtcaaactgg aaacgaacat cctgcatacc gtttttcatt
     1441 acttgtctcc atgtattgta gttttcgtaa tacaaaggaa gtatttcctt ctctaatatc
     1501 tcatatagtt tttccaggtc gtactcatct tgttcataaa cagccatatt agcgtaatcg
     1561 gctttgggca ctacaaaacc gttatggcca tgattgataa actcaggaat ccatccgtca
     1621 tctgtagaaa aattgatagc gccattcatc gcagctgtca taccactggt tccggaagcc
     1681 tcccttggta ctcttggatt atttaaccaa acatcagctg cctgcttcag tcgcttggat
     1741 aagcctaatt cataagctat gagcactgcc acattcttgt aacgtttact gagatgaacc
     1801 agttgattaa actcactgat agccgggtga tcaaccgggt aaggttttcc ggcccatata
     1861 atctgtaccg gatacttact gttactcaat aatttttcaa accgatgttc atcagttgtt
     1921 ataagccctg cccttttata acctgcaaat cgcctggccc atacaatagt aaatacatcg
     1981 gggctgaaga gttttcctgt ctggtcagct actatttcaa atgcccgttt ctttaaatat
     2041 ttcttgcggt catcaattcc gtaatcatct ccggcttcca tgaacttata taactgcttg
     2101 tccgcccaat accgccagtt ttgcgcattc gtgatagaaa taatgggaca tatattttca
     2161 tgcttgcccc acatggctct tgaaacatgg ccgtgcagtt gtgaaacacc attggccagc
     2221 tttgcaaaac gtaaggctgc cagtgaatga ttgaactgat cactgtcatt gccataaaga
     2281 tttttcactt catccactga caggccgcag aaataactca ttttatgaca gaggtaaata
     2341 tcatgctttt cattacccgc ctcctcaggt gtatgcgtgg taaacaccag tctcttttta
     2401 acatcactaa ggttgttgcc aaacttctta tataaataaa aagcggcaga caagccatgt
     2461 gcttcattca ggtgatacaa ctcaggatta aagcctaaaa gatcaatcag tttggccccg
     2521 cccacaccca gcaatatgaa ctgcgctact ttggtagcta cgttagcatc ataaagccgg
     2581 tgcgtaatcg tttgcgatac gtaatcgttt tccggaaggt cggttgacaa tagaaataaa
     2641 ggcgccgttt taaaagtatc cgggtttaaa taccatgcct tgacccaaac aggatgttca
     2701 tgtatagtaa tctgaaactt tattccggta tcttccaaaa agctataaag cttttcattc
     2761 caggttactt ccagggtttg atcctggttt ctttcctggt cataataacc gtatttccat
     2821 aatataccaa taccaatcag gttttgcctg agctcataag cgctgcgcaa atgtgatcct
     2881 gctaaaaaac caaggccgcc ggaataaatc ttcagtggct ggtgagtggc aaattccata
     2941 gagaagtagg ccactttttt agaataacgt tcttcaaccg gatacgggac tgaaaaggga
     3001 gtaaagctca taatgtgttc tttttacacg aagacgcaag ataaaattaa atattcaata
     3061 atactaagtg atgaagcacc ggtatgaaag acttttgcac aaatagtggg aaaataacaa
     3121 gcaaacggtt gataaaaaat atttctgaca ggattacctt ttttgtttac tgcctgctga
     3181 aggttttaca tatttctttc gggcaaagct gccgtcaaaa gaacatttaa gccccctccg
     3241 ggatccgcaa ccttcttctt cttttaacgt aacagcattc gaagagaaga taaatttcag
     3301 gcgacaagga tctccctcct ctttgtattc tgccgtattg gatgatttga tcaatgcctc
     3361 tcctttcaat tcgccggtac aggcaccatt gtctttttca aaatggataa aaaaactgat
     3421 cctgtcattt ttacggccat ccctcaccga cacaaggttc attttaccat tggcgtaatc
     3481 ggccgaccat ttatgttttc ggggtaatgt atcaatcggg ttgatgagtt ctgtgatctt
     3541 atcctccagg gcttctgtca tgatcagcat aaaattctta gccgctgcat tcaacacata
     3601 gacctctttt ccttcactga tggtgccatc cctgttcttt cgggccatgg tttgtgttat
     3661 ggtatatttc ctgtcaagta tggctgactg actggttgaa ctttgcttat ctggccgcag
     3721 caatggcatc ccggcaataa actgttcttt ttcatcaaaa gcaagcagaa aataagccct
     3781 tttgtcggca ctcactgttt tgacaaaaag gtattgttcg gcttttggaa caaccgcttt
     3841 acctaacgca taaatttttg gcttaacacc tttggcaaaa acctttctta aaacagaatc
     3901 aggtacaaac tgtgtaaata ttttataact gatcagtaat gaatcttttt cctttttagc
     3961 aagtgaacta tccgaaaatt gcaccggcag gtttaagggc tgaaaaaatt caataaaatc
     4021 ggaaacttca acaggatctt ctcccgaaag ggatattttt ttcttgccct tgcagcttcc
     4081 cagcccgata accaggcata tcaaaaaaag gaatttcaaa aataatcgca ttggggaata
     4141 tttcttaccg aatgtaaggg attttataat gactggcttc cttcagtagt caagaaaatt
     4201 attttagctt tgcctaaaat aatttttagg catgctaaat tattctgtta gcgaagaaaa
     4261 ttatatcaag gcgatatttc acctgcaaca acaggatgga acggtcacta ccaacgaatt
     4321 ggccggtgaa ttgaataccc gcccggcttc tgttacagat atgatgaaaa aattaaaagc
     4381 caagaaactg gttcattacc agccctacag gggtttcagg cttagtgcag aaggcagtaa
     4441 agtagcctta ggtattattc gtcgtcatcg cctctgggaa tatttcctgg ccgaaaaatt
     4501 aaagtttagc tgggatgaag tgcatgctgt agcagaggaa ctggagcatg tcagcagcaa
     4561 aaagctgatt gaaaaactgg atgaatttct tggctatcct aaagctgacc cccatggtga
     4621 ccctatcccg gatgtaaatg gtaagatcga acactactct tatcaatgcc tcgccagttt
     4681 accggcaaat aaattagtag tggtgagcca tgtcagcaat cagtcaaatg aaatactgga
     4741 cttacttaaa cataaaaaga taagcattgg tacaaaactg gaaataaaga ggcggtttga
     4801 ttttgatgac tccatggaaa ttaagatcgg gcgacagcct gttttcacta ttagtaaaca
     4861 ggtagcagaa aacatcttcg ttaaaataaa ttagtatgga agcattggaa aaattcttag
     4921 ttgaaatcgg accggtgtgg tctgctttaa tcgctaccac ttttacctgg ctggtaacgg
     4981 cagtgggagc ctcatttgtt tttttcttca aaacattaag ccggggtgta ttggacccca
     5041 tgctgggttt taccggggga gtaatggtgg ccgccagttt ctggtcatta ctcaacccgg
     5101 caattgaaat gtcggaaaaa atgtacccgg gccttagctg gatgccagct tcagtgggct
     5161 ttttattagg tgcactattc atttttgtgc tggataaatt tactccccac cttcatatca
     5221 attttggagt ggaagaatcg gaaggggtaa aaaccaaact tcataaaacc actttgctga
     5281 ttttggcgat aacacttcat aatattcccg aaggattagc ggtgggtgtt ttgttcggcg
     5341 ctgctgctat cgggatggaa gatgcatctg tatccggagc tattgcactg gccattggca
     5401 ttggcattca aaattttccc gaaggtatag ccgttgccat gccgnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnaaagaga taaatatact gacaggtcag tgcttggatt tataggtggc ttcctcgtaa
     5701 tgatgatgct ggatgtgggt cttggttaat gatcagaccg ttgaagagcg gtgatgatta
     5761 cgcagcaaat gtttaaaacg gctgaatgtt tcaggcttaa tattcaggta agaggccagg
     5821 tatttttggg gtaccctttg catcaattct gggttacggg ttacaaaatt gatgaatctt
     5881 tctcttggtg tcatttttac caattgcatt tgccagcgat ccttaaggat catggtatgt
     5941 gtaataacaa gcctgcccat atgctccatt ttttgagtcc gggcaaaaac ggattcaagg
     6001 tcatcataag taatcgaagc gaccacactg gactcaatcg cctctacaaa atattctgat
     6061 ggcttacggc tatgaaaaga ttcctggctg tgaatgatat ggccttcaaa agaaatctgt
     6121 gtattgattt cgtctcttcc ttttttgtaa tatttgcgga taaggccttt taggataaaa
     6181 ttgaagtaat tctccgtttc accggctttt gtcaatagtt cccttttgtc aaacttccgg
     6241 atcttaataa agggcagtaa aactgaatta aactcatcgt ctggcaggtc gataaatttg
     6301 cggaggtaat cctttaataa ttgaagcgat tccatactac taattcaggt aaaaggtacg
     6361 actaatttta ttgattcaaa tcaagcccta taattttcct gttgtcaaaa gtcggacaga
     6421 ttaaagacca aaaatgtgat cttcgtcaca tatcggaata ttgtagtttt gcaactttct
     6481 gaaaataaac actaaacaca actgttatgt cgcaaaaaat caaagtggcc aacccggtag
     6541 tagaacttga cggggacgaa atgacacgga tcatctggaa atttattaag gataaattga
     6601 tcctccccta ccttgaactg gatatcaaat attttgacct gggtatagaa taccgggata
     6661 aaacagacga ccaggtaaca gttgatgctg ccaacgctat caagcaatat ggagtgggaa
     6721 taaaatgtgc caccattact cctgatgaag caagagtaaa ggaatttaac ctgaagcaaa
     6781 tgtggaagag ccccaatggc actatccgaa atatactgga tggcactgtt ttcagagagc
     6841 cgattgtgat caataatgtt ccgagattag ttaccaactg gacggcaccc atcatcgttg
     6901 gccgtcatgc ttttggcgat caataccggg ccactgatac agttattaaa ggaaaaggca
     6961 aactcacgat gaccttcacc cccgaaggcg gtggtgaacc acaaactttc gaagtgttca
     7021 atttcaaagg tgatggcgta gccatgacca tgtacaatac agatgagagt atcaaaggat
     7081 ttgcaagaag ctgctttaat atggcgctta gtaaaaaatg gccgctctat ctttctacca
     7141 aaaacaccat tttgaaaaaa tatgatggcc gcttcaaaga tatttttgaa gatatctatc
     7201 aaaatgaatt caaagcacag tttgatgcag ccggtataac ctatgagcat cggctgattg
     7261 atgatatggt agccagtgcg ttaaaatgga atggaaattt tgtgtgggca tgcaaaaact
     7321 atgacggtga tgtacaaagt gacacggtag cccagggatt tggatcacta gggctgatga
     7381 cttctgtttt gattacacct gatggtaaaa ccatggaagc agaagctgct catggcactg
     7441 ttacccgtca ctaccgggat caccaggcag gaaaacctac ttccacaaat cctattgcca
     7501 gtatttttgc ctggacaaga ggtttggcat tccgtggcaa attagacggt aaccagccgc
     7561 ttatcgactt cagtaatgca ctggaagcag tttgtatcga aacagtggaa cagggcaaaa
     7621 tgaccaagga cctggctgtt tgtatccatg gcaataaagt aaaccatggt gagcattacc
     7681 tgtataccga agagtttttg gaagccatcg atgaaaacct gaagaagaaa ctcggcaagt
     7741 aaagtcaatt aaaaaataga atgaatcgct gcgagacttc ccgtctcgca gttttatttt
     7801 atggttataa tatttgtagc ctaaataatt tcatagccta aacaaaaaag ccacccgctg
     7861 aaacgaatgg ctttttgcag ttgtattgaa aaggtttaca ccttgaagtg agcaaaagct
     7921 ttgttagctt ccgccatacg gtgtgtatct tcttttttct tgaaagcagc gccttcacct
     7981 ttgctggcgg ctacgatctc attggcaagt ttatcagcca tnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacctctgaa
     8101 ggaatctgga aagtagcacc accgatacga cggctgcgca cttcaacagc aggagttaca
     8161 ttggccaggg ccttcttcca cacttcataa ccatcttcat tggtaaactt ggccacttta
     8221 tccagtgcat catagaaaat agtaaatgct ccgcttttct ttccttccca catcaggttg
     8281 ttgacaaaac gggtaaccag tttatcgtta aactttggat cgggtgctaa agggagtttt
     8341 tttgcttgtg ctttacgcat gaatgttaat ttttaaagta tctgctgtag ctgaatttct
     8401 tcaagtatta aaactttact cagtacaaac tatgattgaa attatttttt agccttttct
     8461 ttcttagtac catacttaga tcggctcttc ttcctgtctt tcacaccggc agtatccaat
     8521 gagccacgaa caatatgata acgaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnatagag tgctcttgca ggttgtgacc ttcaccgggg atataggcaa tgacttctac
     8641 tttattggtc agacgaacct tggctacctt acgcaacgct gagtacggct tcttaggtgt
     8701 tgtagtatat acccttgtac atacgccacg gcgctgaggg cattgatcca aagctctgga
     8761 tttgctctta gccctgataa tttctcttcc ttttcttact aattgttgaa ttgtaggcat
     8821 tcttgaccct tatttttggg acggcaaagg taaaggcagt aaccttattc tccaaaaaaa
     8881 gcaggctgat ttttcagata ttgatcttct tggggaaaaa taagccccgg ttatttgacc
     8941 agggcttaaa atttaatata atttcctata aatcaagcac tcttaaactt tctgcatcca
     9001 gttgtatttg tcttctttgt ggcctgttcg aatatctgtc agccattgtt tcagggctgg
     9061 tgcagttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gtagcgtaat tctttaatga
     9121 aagaaatagt ggccgctgta cccgttccaa aagcttcata gaaaatgccg gctttatacg
     9181 catcgatcag gtcatctata ctgatatttt tttcttcaac agtaaagccc atctcctgta
     9241 aaagagttat agcactcagg cgggttactc ccggcaaaat agtacccggc tccaggctgg
     9301 gcgtaatggc cgtattacca atgatgaaga aaacattcat tgttccgcat tcctgtacat
     9361 atttatgttc aaacggatcg gtccataaca cctggtcata tccttttttc tttgcttccg
     9421 ccgtggcata catggcagca ccatagttgc ccgcagcttt cgcataaccc acaccacctg
     9481 ttacagctct tacatatttt tcttctacgt aaatacgcat cggcgcactg tagtaaggac
     9541 ctgaagggct cagaatgatc atgaacttat agttctcaga aggacgaaca ccaatcattt
     9601 catctgatga gaacatgaac ggcctgatat acatagagtg atcttgctgt gtaggtatcc
     9661 agttcctgtc cagtcctact aactgaagca ttccgtccat gaacagttct tccggtacgg
     9721 ctggcatggc cattcgctcc gctgaaatat taaaacgttt gaaattatca tacgggcgga
     9781 aaataaaagc ttcgccattt ttatccttat acgctttaat gccttcaaaa atggcctgcc
     9841 cataatgtag ggcagccaac gaggggctca ggagcaaagg ctggtaaggt ttgatctcaa
     9901 tatttttcca ttccccgttt tcataatctg cttccagcat gtggtcggag aaaaatttac
     9961 caaaggggag attttccagg ttcaaatcct gcagtttact gcgttctgcc ttgcgaattg
    10021 aaatatccat agctgccacc ataaaacttt atttttgacg ggtcaaagaa acgaaaaaaa
    10081 ctaacactca tttgtaataa ttatcatccc ggatatggac ctgaatcata taaaactgcc
    10141 agctactgtc ataaatgaac tttataaaac agtattggtg gaaccggtac aaaataccgg
    10201 gatgaaggaa gcacctgcac ctgcagaaaa gccagtgata accagcgctg aagccacaac
    10261 acctgagtgg aaatcactgg gctcaaatga aaaaaatatt ttaattgtcg tcagcaattc
    10321 agatgcagtt tatttaccgg acaatgacct gaatttttta accggtgtgc ttggtgcctg
    10381 taaactcagc ctggccgatg tggctgtcgt aaacaggttc cattacccgg aagccggtta
    10441 taaagaactt attgcttatt tcaaaagcag ggtgatatta ctgttaggag ttgagccggc
    10501 tgctatggga ttacccatca attttccgca ttaccagttg caggcatttc agaacaatac
    10561 ctttcttttt accccgccac tggaagagat agaaaaagac aaactggaaa aaagtaaatt
    10621 atgggtaagc cttaaaagac tctttaatgt ataaacacat gaagctgatt tttgccacta
    10681 acaaccagca caaagtggat gaaataagaa atgtgttggg ccaaactctc gacattatca
    10741 cactgaaaga agccggcata gaggcggata tccccgagcc ttatgatacc ctggaggcca
    10801 atgcatcggg caaatcagcc tatatatttc aattgacagg cgccgattgt ttcagcgagg
    10861 atactgggct cgaagtagcc gccctcaacg gagaacccgg tgtgaaaagt gccaggtatg
    10921 caggcgaaca ccgttctttt gatgccaata ttgataagct attgagtaat ttggccgcca
    10981 agcaggatcg ttcggcccgg ttcaggactg ttatatccct gttaattgat ggaaaggaaa
    11041 tgttgtttga aggcatttgc aacggacaga taataactga aaaaagaggc gaattgggct
    11101 ttggctacga tccggtcttt atcccggagg gaagtgaccg tacttttgct gaaatgagcc
    11161 tcgaagaaaa gaaccgtttc agccatcgga aaaaggcaac tgaaaaactg gtagcatttt
    11221 tgaataagtt atcaacaaac cactgatttt gacaccagaa cgcaaccaaa tcatccccga
    11281 aagcgacctc attaatggat gcctgcaagg caaccggcgc atgcaggaag aactctaccg
    11341 ccgtttttct cccaggatgt atgcagtttg cctgcgctat gccggtaatg ccgaagaagc
    11401 agaagatatt ctgcaggaag gtttcatcaa ggtctttaaa aagttagaca gtttccgggg
    11461 cgacggctcc tttgaaggat ggatccgaag aatatttgtg aatactgcca ttgagcattt
    11521 ccgccgcaaa agatacctga tgcccgttac ggaaaaagaa gaaaacacta ttgaaggcaa
    11581 gtacacgtcg gtgctggacg aactgggagc caaagatatc atggcactca tccaggaatt
    11641 gtctcccggt taccgtacgg tcttcaacat gtatgtggtg gaaggataca cccacaagga
    11701 aattgccgat atgctgggca tcagcgaagg caccagcaaa tcgcagctnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg acagttatgc cctgcagcac
    11941 cattttccta agaaattaca aacggttgaa atcgctcccc ctgcctattg ctggaataat
    12001 atagcagcta cccttgatgg taaaggggcc gaagctgctc tggcagccaa actacataaa
    12061 gcttccgtcg ttccgcctgc agctgtttgg caaaacattg aagcagcgct tgacgaggag
    12121 accaatctgc c
//
LOCUS       DAORLS010000060        12044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2828_length_12044_cov_2.417966, whole genome shotgun sequence.
ACCESSION   DAORLS010000060 DAORLS010000000
VERSION     DAORLS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_12044_cov_2.417966"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..649)
                     /locus_tag="PK977_06445"
     CDS             complement(23..649)
                     /locus_tag="PK977_06445"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRF17786.1"
                     /translation="MAIPAVYLAIVWNKLPEKVAVHFDLKGNPDRFGNKSELLVTTIL
                     LVAMNVLVFLVLTNIYRIDPKKYAAENKDRLVKIAFAVVLFMSGILCMIIYSASNGNI
                     NFSTGIIFSGTGLLFAVIGNYLPNLKPNYFAGLRLPWTLENEDNWRKTHQLAGKLWFA
                     GGLLLAAVCLFLPPVAAIAVFFAVMMIIVIIPCVYSYRLYKEQKRTIK"
     assembly_gap    778..821
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1237..1671
                     /locus_tag="PK977_06450"
     CDS             1237..1671
                     /locus_tag="PK977_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17787.1"
                     /translation="MKKFILTLAIAVTTLSAFAFKGVNEKVLKSFQSDFNTAKEVVWT
                     AGSNYYKAAFTYNEKHVFAYYSADGELLGVTRYLSPADMPMHLQTELKKDYNNYWVSD
                     LFEVSKPEGTAYYVTLENADTKLVLKAENGSDWNYFQKVKKS"
     gene            1919..2362
                     /locus_tag="PK977_06455"
     CDS             1919..2362
                     /locus_tag="PK977_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17788.1"
                     /translation="MKKLLLSLSLVLAVAAGTAFASPDPSINEAVKESFKKEFAGAEL
                     VEWREVGNHLKATFVLNGYRTEAYFSQEGELQGSARSLFFSQLPLVVMSAVDKRFAGA
                     DIIDVNELNSAAGTYYRITLETGKKKYRVRVDADGTVVDTERLRK"
     gene            2504..>3158
                     /locus_tag="PK977_06460"
     CDS             2504..>3158
                     /locus_tag="PK977_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRF17789.1"
                     /translation="MNKGPISQFIQHHYRHFNAAALVDAAKGYEQHLLEGGKMMITLA
                     GAMSTGELGISLAEMIRQGKVDIISCTGANLEEDVMNLVAHSHYKRIPNYRDLTPQEE
                     WDLLENHYNRVTDTCIPEEEAFRRLQKHLVKQWKDAEAKGERYFPHEFLYKTVLSGEL
                     QQYYEINPKDSWIVAAAEKNIPIVVPGWEDSTTGNIFASYVIKGELKASTVKSGIEYM
                     "
     assembly_gap    3159..3176
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3177..3481
                     /locus_tag="PK977_06465"
     CDS             <3177..3481
                     /locus_tag="PK977_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRF17790.1"
                     /translation="YRHNSSGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWS
                     YFCQISDSTTSYGSYSGAVPNEKITWGKLDINTPKFIVESDATIVAPLIFAWILGW"
     assembly_gap    3501..3540
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3575..>3850
                     /locus_tag="PK977_06470"
     CDS             3575..>3850
                     /locus_tag="PK977_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17791.1"
                     /translation="MEAFKKYETILLEEPGNGPTLNSLGWLYKTQVEDFKKAESFYLA
                     SIKYSPLYPHAYNNYATLLTDMERFEELTIHLQKCFEVPTIEKSWIYL"
     assembly_gap    3851..3860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3861..4040
                     /locus_tag="PK977_06475"
     CDS             <3861..4040
                     /locus_tag="PK977_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17792.1"
                     /translation="FGFMEELKLNFIEAITYYERAALITLNDDKLKEYNEHIQRCNAK
                     LEMSKRHAKWLEKMK"
     gene            complement(4076..4534)
                     /locus_tag="PK977_06480"
     CDS             complement(4076..4534)
                     /locus_tag="PK977_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17793.1"
                     /translation="MSEYQNNPEPDAPKENEIADYYEGVKKLEMDGHETGIKKARNAL
                     FITAGLLLLGEIIAISTSGAEVTPLVIGIIAAEVGVFIGLAFWTKTKPYAAIITGLIL
                     FILLWIVAIIVNDDGGQSIYKGILVKAIIIYTLVSALKPAKEWENLKKNS"
     gene            complement(4721..>5582)
                     /locus_tag="PK977_06485"
     CDS             complement(4721..>5582)
                     /locus_tag="PK977_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF17794.1"
                     /translation="ISPAIAAQITKTETNFLEKAKNGEIASKNYSANVDLSYSKESKT
                     TYASNVIGVVEGSDLKDEYVFVTAHYDHLGMRDTVIYYGADDDGSGTVAVMEMAEAFA
                     KAKEQGKGPRRSIVFMAVSGEEKGLWGSSYYTSNPIFPLDKTTVDLNIDMIGRADASR
                     TTGDSMNYVYVVGDDKISTDLKVISEATNKLYTKMELDYKFNDPKDPNRIYFRSDHYM
                     FAEKGVPIIFYYDGMLKADYHRPTDTPDKIYYPLLQKRAQLVFYTAWEMANRNEMLKR
                     DIKLEVPKGF"
     assembly_gap    5583..5748
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5749..6269)
                     /locus_tag="PK977_06490"
     CDS             complement(<5749..6269)
                     /locus_tag="PK977_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HRF17795.1"
                     /translation="MLRKIIALFFITLPLLAVAQKKNKDAVKAAATITTDDLKKHLYI
                     IASKEMQGRGTPSPGLDKAADYIEAHFKAIGLKAGNNGSYRQQYPLFKDSMISSALKV
                     NGTAYELNTSFQPQASNYTADMRFSEVVFAGYGISDGENRDDYKDLKVAGKLVMIIDG
                     TPSGYKPSQQGFGS"
     gene            complement(6349..6804)
                     /gene="rpiB"
                     /locus_tag="PK977_06495"
     CDS             complement(6349..6804)
                     /gene="rpiB"
                     /locus_tag="PK977_06495"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRF17796.1"
                     /translation="MHAAFDLHKPIAIGSDHAGYDYKEDLISFLEGKGLLFKDFGTHS
                     KDSVDYPDFAHPVASAVESGEAAFGILLCGSANGVAITANKHQGVRAAICWGEELAKL
                     SREHNDANIICIPARFVREGDAEKMVALFMTTDFEGGRHANRVKKIACA"
     gene            complement(6900..7754)
                     /gene="tatC"
                     /locus_tag="PK977_06500"
     CDS             complement(6900..7754)
                     /gene="tatC"
                     /locus_tag="PK977_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRF17797.1"
                     /translation="MALLDFFNKRKKDTSAEMSFIDHLEELRWHVIRSVIAVLVGAVV
                     IFIYSDFVVNDILFAPTRSDFISARWLCRFGHSIGIGDTLCFQQVDAKFLETTMTGQF
                     IASFTLAFMGGFIIAFPYIFWEFWKFVKPALSDKELKKTRGVIFWVSLLFFTGVAFGY
                     FILTPFMVNFYFNYKLSPQIQIMPSFSDYLENLIYTTVGIGVLFQMPLLVAVLARIGI
                     VTGRFLRKYRRHAFVVILIAAAIITPSTDPFSLTIVTIPLYLLFEASIIIASRINKSQ
                     ELEQKEWS"
     gene            complement(7872..>8079)
                     /locus_tag="PK977_06505"
     CDS             complement(7872..>8079)
                     /locus_tag="PK977_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRF17798.1"
                     /translation="KPHELDYLEEKYGFEFGEEESETLSGYIINYYETIPRQKERIII
                     DDYEFDILNVSDTRIETVKMRKLK"
     assembly_gap    8080..8089
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8090..9128)
                     /locus_tag="PK977_06510"
     CDS             complement(<8090..9128)
                     /locus_tag="PK977_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRF17799.1"
                     /translation="MIEWNAILFFVLTLVLMAFFAGIEMAYYSANRLSLELKKKQGGA
                     NAQRLTKFIDSPAKFLGTTLIGYTIFLVFLGLQFSVVMRPIWSYLHVGSDTVHLVVEI
                     AFTTLIVLVFAEYIPRAWFRAKSNSWLASLSPVIDFFYGLFSPIATSLIDLSEWILKY
                     VMNIRLDKNKEGFTRNDLKNLFLIKSEDERQERNTQLMENAQELPRVKIRQCLVPRKE
                     ITGIESSASVDEMKAKFIDTKLSKLVVYENNIDHIIGYVHQLDLFKKPAAIKDILLPI
                     PAVPESMSASDLISKFTRDRKSIAWVVDEFGGTAGIITMEDVLEELFGEIQDEYDTEE
                     FVEKQLSENEYI"
     gene            complement(9234..9815)
                     /gene="gmk"
                     /locus_tag="PK977_06515"
     CDS             complement(9234..9815)
                     /gene="gmk"
                     /locus_tag="PK977_06515"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRF17800.1"
                     /translation="MAVYLNKLIIIAAPSGAGKTSVTRHLLKAFPDDLSFSISCATRQ
                     PRHHEKQGVDYYFISADEFRQRIENKEFAEWEMVYHDKYYGTLKSELERIWKQHKAPL
                     LDVDVKGGINIQDQYPQQSLSLFIEPPSIAELEKRLKARGTESAESLKARIDKASYEL
                     SFKNRFDKIIINDKLEDACREAEAAVREFLMSS"
     gene            10690..10956
                     /locus_tag="PK977_06520"
     CDS             10690..10956
                     /locus_tag="PK977_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRF17801.1"
                     /translation="MKKSRFTETQITGALKAYEAGKDIQQICREMNINRATFYNWKKK
                     YSGMDADLLRQFKELQRENTELKRMYADLSLDHNILKKVIEKKL"
     gene            10977..11762
                     /locus_tag="PK977_06525"
     CDS             10977..11762
                     /locus_tag="PK977_06525"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRF17802.1"
                     /translation="MAAEIVQEEQVSIVRACRIVQLDRSLFYYRSMKDDSEVEAKLTS
                     YLDNKVLCNRGCPEYYKRMRREGLMWNHKRIERVYRKMGLSKRRKRKRRLPNPEKRPL
                     VQPVLMNETWSMDFVEDRLENGRKIRTLNILDDFNREALAMEVSFSFPAEQVIEVINQ
                     CIEWRGKPKRIRSDNGTEFVAIKTQQFFSNKAIEHIPIQKGKPMQNGYVERFNRSYRE
                     GVLDAFILTSIEQAREETTVWMEDYNRNHPHESLGDKPPLEYG"
     gene            complement(11798..>12044)
                     /locus_tag="PK977_06530"
     CDS             complement(11798..>12044)
                     /locus_tag="PK977_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17803.1"
                     /translation="GFTLINIFFAYSFDQLNTKKQVIEVLGRLGIFVALGLVLTHLMR
                     LVIININLLQKKLEKQIGIFILITFCKSPLFPTGLNY"
BASE COUNT         3416 a         2383 c         2646 g         3311 t
ORIGIN      
        1 aaatatttgt ttttcatatc aactatttaa tggttctttt ttgctcttta tacaaacgat
       61 aagaatacac acagggtatg atcacaatta tcatcatgac tgcaaaaaac actgcaattg
      121 ctgctaccgg tggcaaaaaa agacagaccg cagcaagcaa caacccgcct gcaaaccata
      181 attttccggc cagctgatgc gtctttctcc agttatcttc gttttctaat gtccatggta
      241 aacggagccc ggcaaaataa ttaggtttca gattgggaag gtaattgccg atgacagcaa
      301 ataataatcc tgtaccactg aagattatac ctgtgctgaa attgatgttt ccgttggaag
      361 cactataaat aatcatacag aggatgcctg acatgaacag tactactgca aaagcaatct
      421 tcaccaggcg gtctttattt tcagcggcat atttttttgg gtctatcctg taaatgttgg
      481 tgagaacaag aaagaccaat acgttcatgg ctaccagcag aatagtagta actaacagtt
      541 ccgatttatt gccaaaccgg tcagggtttc ctttcaggtc aaaatgtacc gccacttttt
      601 caggcagctt gttccagaca atggccaggt aaactgctgg tattgccatt atcggccaaa
      661 tcaatttttt caggatgctg ttcatatcta tttctttttt gatttgaatt gcataaacca
      721 ttttaatata tcgtccacta ctgtggtatt caaagaatag taaacaaatt gcccttcnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgagagat actgggaaaa
      841 gacatattaa actgatctgc aatttctccg gccgtcatat ccttttcctg caacagctcc
      901 agtatttctc gtcgggtggg atcgttcagc gctttgaaga cactgttcat aaagctttgt
      961 aattatatat ttagacaaat gtctaaatat tatttcactc ttcaaagaat tttttttcga
     1021 atcacataaa catgtgatgt atgtggatga ccggtattta aaaatggcca gcggttaatt
     1081 ttcagcaact tctgttaaca gcaattaaca cagcactcca ttaatcacaa atgccttgtg
     1141 acggggtgta acctaaaata cctttgtacc gtcacacata gcaaatgaca caaacgataa
     1201 caaaacaatt gaccttaacc ttctaaataa ataatcatga aaaagttcat cttaaccctg
     1261 gcaatagctg ttaccacact tagtgctttt gcttttaagg gtgtaaacga aaaagtgctg
     1321 aaatcattcc aatctgattt caatacagct aaagaagtgg tgtggacagc tggtagtaat
     1381 tattacaaag ccgcttttac ctataacgag aagcatgttt ttgcttatta ctctgcagat
     1441 ggagagctgc ttggtgtaac ccgttatctc agccctgcag atatgccgat gcacctgcag
     1501 acagaattga aaaaggatta caacaattac tgggtaagcg acctgtttga ggtttcaaag
     1561 ccagaaggca cagcgtacta tgttacatta gagaacgccg atactaaatt agtattgaaa
     1621 gcagagaacg gcagcgactg gaactatttt cagaaagtaa aaaagtctta aagacaaaat
     1681 ctttctgtca gcatacaaaa cagttttggt gtgtggtaaa aaacaagccc cggggtgttc
     1741 attgctttcg gggttttgtt tttattaaaa gctctttcgt taatgaatgt gcccgattgt
     1801 taagctgttt aacattgact tccgcaaatc acaattcact tgacttcgct ttgataccat
     1861 tatacatttg aattgtaaaa tcagcaatca acaaacatta atcattaaac cttaagccat
     1921 gaaaaaatta ttattatccc tgtcattagt attggcagtt gctgcaggta cagcttttgc
     1981 cagccctgat ccttctatta atgaagccgt aaaagaatcc ttcaaaaaag aatttgctgg
     2041 tgcagagctg gttgaatgga gagaagtggg taatcattta aaagcaacat ttgtattgaa
     2101 tggttatcgt acagaagcct acttcagcca ggaaggagaa ttgcagggaa gtgcccgttc
     2161 acttttcttc agccagttac ctttagtagt gatgtcagcc gttgacaaac ggtttgccgg
     2221 agcagacatt attgatgtaa acgaattaaa cagtgctgcg ggaacctatt acagaataac
     2281 gctggaaaca ggaaaaaaga aataccgggt aagagtggac gctgatggca ctgtagtgga
     2341 tacggagcga ttaagaaaat agagtgattg aatagccgga aaaacaaaat tccgtctcct
     2401 tgtaggagcg gaatttttgt ttgtgtaaaa ctgtaatttt gccatcccgc attgtctcgc
     2461 cttattgcga tctgaaggcg gttttacaaa aacgtattgt aaaatgaaca aaggtccgat
     2521 ctcacaattc attcagcatc attatcgtca cttcaacgcc gcagcactgg tggatgctgc
     2581 caaaggctat gagcagcatt tgcttgaagg cggaaaaatg atgatcaccc tggccggggc
     2641 catgagtacc ggtgaactgg gtatttcact ggcagaaatg atccgccagg gaaaagtaga
     2701 tattatcagc tgtaccggcg ccaaccttga agaagatgtg atgaacctcg tagctcattc
     2761 acattacaag aggatcccta actaccgtga cttaacaccg caggaagagt gggatctgct
     2821 cgagaatcat tacaacaggg ttactgacac ctgcattccg gaagaggaag cctttcgccg
     2881 cctgcaaaag cacctggtga aacaatggaa ggatgccgaa gcaaaaggag aaagatattt
     2941 tcctcatgaa tttttataca agacagtatt aagtggtgag ttacagcagt actatgagat
     3001 taatccgaaa gacagctgga tcgtagcggc agcagaaaaa aatataccaa tcgttgttcc
     3061 gggatgggaa gacagtacaa caggtaacat ttttgccagc tatgtaataa aaggagaact
     3121 gaaagcaagt actgtaaaga gcggcattga atacatggnn nnnnnnnnnn nnnnnnggta
     3181 ccgacataac tcatccggta aaggtgttgg cttcttccag attggcggtg gtattgccgg
     3241 cgattttcct atatgtgttg tacccatgat gtaccaggac ctggagtggc atgatgttcc
     3301 tttctggagc tacttctgcc agatcagtga ttcaaccact tcttatggtt cttattctgg
     3361 tgctgtgcct aatgaaaaga taacctgggg caaactggat atcaataccc ccaagttcat
     3421 tgtggaaagc gatgccacta ttgtagcgcc gttgatcttt gcatggatat tgggctggta
     3481 aatttataac tatgaacaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 tgaagctgaa gcggatatta agaataacaa ctatgtggaa gctttcaaaa agtatgagac
     3601 aatacttttg gaagagccag gtaatggccc gacgctaaat tcacttggtt ggctttataa
     3661 aacgcaggtt gaggatttta agaaggctga atcattttac ctggcaagta taaaatacag
     3721 cccattatat ccgcatgcat ataacaatta tgcaacactc ctaacagata tggaacgttt
     3781 tgaagaactt acaattcatc ttcaaaaatg cttcgaagta cctacaatag aaaaatcatg
     3841 gatttacctg nnnnnnnnnn tttggattta tggaggagtt gaagctgaac ttcatagaag
     3901 caataactta ttatgaaaga gcggcattga ttacacttaa tgatgacaaa ctaaaagaat
     3961 ataatgagca tattcagcga tgcaatgcaa aacttgaaat gtctaagcga catgcgaaat
     4021 ggctggaaaa gatgaaatga ttattaaccc cgctttaagg cggggttaat aattattagg
     4081 aattcttctt caggttctcc cattcttttg ctggctttag tgcactaacc aatgtataaa
     4141 taataattgc ttttaccagt atgcccttat aaatgctctg accaccatca tcgtttacaa
     4201 ttatggcgac tatccatagt aatatgaaga ggatcagccc agtgataatg gcagcataag
     4261 gttttgtttt tgtccagaat gcaagcccta tgaaaacgcc aacttcagca gctattatgc
     4321 caataacaag tggtgttact tctgctccgg aagtacttat agcgataatt tctcccagca
     4381 gtaaaaggcc ggcagtaata aaaagagcat ttcttgcttt ttttatgcca gtttcatggc
     4441 catccatctc cagctttttc acaccttcat agtaatcggc aatttcattt tcctttggtg
     4501 catccggttc aggattattt tggtactctg acattttttt gtttgaatat agaaaaataa
     4561 aaaactctgt ccaatttttt accggacaga gtgtatatgt ataagggagg attaaggcgt
     4621 atcagtctca acggaaatgt gaccaaagac gttgccaccc gtttaattac ataataaaaa
     4681 acctccccga ttggggaggc ttcttattgt ttgtttatta tcagaatcct tttgggactt
     4741 ccagttttat atcccgcttc agcatttcat tccggttggc catttcccag gcggtataga
     4801 ataccagctg ggctcttttt tgcagcaacg gataatagat tttgtcaggg gtatcagttg
     4861 gccggtggta gtcagccttt agcatgccat cataataaaa aatgatgggg actccttttt
     4921 cggcaaacat ataatgatca ctgcggaagt aaatccggtt cggatctttc ggatcgttga
     4981 acttataatc gagttccatc ttcgtataaa gtttgttggt tgcctcactg atcactttca
     5041 ggtcagtgct gatcttatca tcacccacca catacacata gttcattgaa tcgccggtag
     5101 tacggcttgc atcggccctg ccgatcatat caatgttcag gtcaaccgtt gttttatcca
     5161 gcgggaatat cgggttactg gtataataac tggaacccca taatcccttt tcctcaccac
     5221 tcactgccat gaatacaatg gagcggcggg gacctttgcc ctgctctttt gctttcgcaa
     5281 atgcttctgc catttccatt acagctacag tacctgaacc gtcatcgtct gcaccgtaat
     5341 agatcaccgt atctctcatg cccaggtgat cgtaatgtgc tgttacaaaa acatattcat
     5401 ctttgaggtc actgccttct actacgccta tcacattgga tgcataagtg gttttggatt
     5461 ctttgctgta tgaaaggtca acattggcac tgtaattttt agaagctatc tcaccattct
     5521 ttgctttttc aaggaaattg gtttctgttt tagtaatctg tgcggcaatg gcaggggaaa
     5581 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga accaaagccc
     5761 tgctgcgaag gcttataacc ggacggtgta ccgtcaatga tcatcactag tttaccagcc
     5821 actttcaggt ctttgtaatc atccctgttt tcgccatcgc tgataccgta accggcaaat
     5881 accacttcgg agaaacgcat atctgccgta tagttggagg cctgtggttg gaaagaggta
     5941 tttagttcgt aagctgtacc atttactttc aaagcactgc tgatcattga atctttaaac
     6001 agcgggtatt gctgcctgta acttccgtta ttgcccgcct tcagcccgat agctttaaaa
     6061 tgtgcttcaa tataatccgc tgctttatcc aaacccggcg aaggagtacc tcttccctgc
     6121 atttccttgc tggcaatgat ataaagatgt tttttcaaat catcggtggt aatagtagcg
     6181 gctgctttta ctgcatcttt gtttttcttt tgtgcaacgg ccagtaatgg cagcgtaata
     6241 aagaataaag caataatttt tcgtagcatc atcgttgtgt aatattagtt agctataaaa
     6301 ataatccctt cccgctaagg aagggacaaa atatggatga ccggtagctt atgcgcaggc
     6361 gatcttcttc acccggttgg catggcgacc gccttcaaaa tcagtggtca taaaaagtgc
     6421 aaccattttc tctgcatcac cttcccgcac aaaacgggca gggatacaaa tgatattggc
     6481 gtcgttgtgt tctcttgaca gtttggccag ctcttcgccc cagcagatag cagcccttac
     6541 tccctggtgt ttatttgctg tgatggccac accattggcg ctgccacata acagaatgcc
     6601 aaatgcagcc tcgccacttt caacagcaga tgcaacagga tgggcaaaat cgggataatc
     6661 aacagagtct ttgctatggg taccaaaatc tttaaacaac aatccttttc cttctaaaaa
     6721 agagatcagg tcttctttat agtcataacc ggcatggtca ctaccgatag caatgggttt
     6781 gtgcaaatca aaagcagcat gcataattgt caggagttta tagtatggaa ttaaaagttg
     6841 agttagtgta aagttaggaa actccacact ccccactcag tactgttgaa tgataaatat
     6901 cagctccatt ctttttgctc aagttcctgg ctcttgttga tgcgggaagc gatgatgata
     6961 ctggcttcga ataatagata aagagggata gttacaattg tcaggctgaa ggggtcggtg
     7021 gacggtgtaa tgatggcagc agctatcagg attaccacga aagcatgacg gcggtatttg
     7081 cgcaggaatc ttccggttac aataccgatc ctggcgagta cggctaccaa caggggcatc
     7141 tggaacaata cgccaatacc tacagtggta tagatcaggt tttccaaata atccgaaaaa
     7201 ctgggcatga tctgtatctg ggggctcagt ttgtaattga aataaaaatt caccatgaaa
     7261 ggggtgagga tgaaataacc aaaagccaca ccggtgaaga ataaaagtga cacccaaaag
     7321 ataacacccc ttgtcttctt taattcctta tcagacaaag caggttttac aaacttccag
     7381 aactcccaga aaatataggg gaatgctatg atgaatccgc ccataaaggc aagggtaaaa
     7441 gaagcaatga actgccccgt cattgtggtt tccaggaatt tggcatctac ctgctggaaa
     7501 caaagcgtat ctccaatgcc aatagaatgg ccaaaacggc acagccacct cgcagaaata
     7561 aaatctgacc gggtgggggc aaacaggata tcatttacca caaaatcgga gtaaatgaaa
     7621 atgaccacag ctccaaccag tacggctatc accgaacgaa tcacatgcca gcgcagctcc
     7681 tccaggtggt cgatgaaaga catctccgca ctggtatctt ttttccgctt attgaagaaa
     7741 tcgagaaggg ccattttttt agaaagtgtc gcaaagataa gcggggattc gggattcggg
     7801 ttacgagttt cgggtgcaga cccttgctct caaatacacc tcttccccat taactaatta
     7861 accaattaac cttacttcag cttacgcatc ttcacggttt caatccgggt gtcgcttacg
     7921 ttcaggatgt cgaattcgta atcatcgatg atgatacgtt ctttctggcg gggaatagtt
     7981 tcgtagtagt tgatgatata gccagaaagc gtttctgatt cttcttcacc aaactcaaag
     8041 ccgtattttt cttccaggta gtccagctcg tgcggcttgn nnnnnnnnna tatatattca
     8101 ttttcagaaa gctgcttttc tacaaactct tctgtatcgt attcatcctg tatttcgcca
     8161 aacaattctt ccagcacatc ttccatggta atgataccgg cggtgccgcc aaattcatct
     8221 actacccagg caatgctttt acggtcacgg gtaaacttgc tgatcaggtc ggatgcactc
     8281 atactttccg gtacggcggg gatgggcaac aagatatctt ttattgctgc cggtttttta
     8341 aaaaggtcca gctggtgcac atagccgatg atatggtcaa tattgttttc atacaccacc
     8401 agcttactca gcttggtgtc gatgaatttt gctttcattt catctaccga tgcactgctt
     8461 tcaatacctg taatctcttt gcggggcacc aggcactgcc ggatctttac tcttggcagc
     8521 tcctgtgcat tctccatcag ctgcgtattg cgttcctgcc tttcatcttc tgattttata
     8581 aggaaaagat tttttaaatc gttgcgggtg aagccttcct tgtttttatc cagccggata
     8641 ttcattacat acttcagtat ccattccgac aaatcgatga gtgaggtggc aatgggggag
     8701 aacagaccgt aaaagaaatc aataacaggc gaaaggctgg ccagccagct attacttttt
     8761 gccctgaacc atgcacgggg aatgtattca gcaaagacca gcactattaa ggtggtaaaa
     8821 gcgatttcca ctacaagatg aaccgtatca gacccaacat gcaggtaact ccagatgggg
     8881 cgcatcacaa cgctgaactg aagcccgaga aagacgagga aaatagtata accaatgagg
     8941 gtagtgccga gaaatttggc cggcgaatca atgaatttgg tgaggcgctg agcattggca
     9001 ccgccctgtt ttttcttcag ctccaggcta agacggttgg cgctgtaata ggccatttcg
     9061 ataccggcaa agaaggccat aagcaccagg gtgagtacaa aaaacaatat cgcattccac
     9121 tcgatcatgc tgcgaagata ccgggtgttg gggacatagg gggatggttg acaggttaac
     9181 aagttaatag gttgaaaggg agctccctgt taacttcctg gcatttcaac ttgtcaactg
     9241 ctcatcaaaa actcccttac tgccgcttct gcttcccggc aggcatcttc cagtttgtcg
     9301 ttgatgatga ttttatcaaa ccggtttttg aaggagagtt cgtaagaggc tttgtcgatc
     9361 cttgccttga gtgattcggc tgactcggtg ccccttgcct ttaatctttt ttccagttcg
     9421 gcaatggaag gcggttcgat gaacaatgac agggactgct gcgggtactg gtcctgtata
     9481 ttgatgccgc cttttacatc tacatcgagc aggggggctt tgtgctgctt ccagatgcgt
     9541 tcgagttcac tttttaaagt gccataatat ttatcgtggt acaccatttc ccattcggca
     9601 aattccttgt tttcgatgcg ctgcctgaac tcatcggcgc tgatgaaata ataatccact
     9661 ccctgctttt cgtggtggcg gggctggcgg gtagcgcagg aaatggaaaa cgaaagatcg
     9721 tcagggaaag cttttagcaa gtgccgggtg acggatgttt tgccggcgcc ggatggtgcg
     9781 gcgatgataa tgagtttgtt gagatagaca gccatgcgca aagaaaggaa aatttacggg
     9841 ttacgggatg cgggattcga gttacgggat tcgggaggcg ggttacgggt ctttcaagat
     9901 gaatttgatg actttcgatg cgagttggag ccgttccgag atgaatttga tgtctttaga
     9961 tacggcaggg agtcgttcag atacgattcg gaggtctttc gatgcgaacc ggagcccttc
    10021 cgatgtgaat ttgatgtctt ccgatgcgag ttggagccgt tccgagatga atttgatgtc
    10081 tttagatacg gcagggagtc gttcagatac gattcggagg tctttcgatg cgaaccggag
    10141 cccttccgat gtgaatttga tgtcttccga tgcgagttgg agtcgttcag aaatgaattt
    10201 gatgtcttcc gataggagct atgtaagggt agaaatttct taaatgattg gtttttaaca
    10261 gtttttctgg aaaaggcatt tttgtgggtt gtctgaactt ggatactgaa tcaagttgag
    10321 tacaagtttg gggagattat tgggaatatg agaacaggtt gacaggtagc tcaataaaat
    10381 ggagaaagtt ttgaataaaa cgagttttcc ccgacgggcc taggcgacaa gatgagtgga
    10441 tgcaattttg ctgatctgtg ttaatgaaga tggggacaat tagctcaacg ttgaagcatt
    10501 ggcgatgtgg cggtattctg tgttccgtct gcctggtgcc gctgctgatt aaagatacaa
    10561 aagctcatcg tatattccgg tgctcggcgt tattcgtctg acgcagctga ggttgagtat
    10621 gtaagtcccc cgaaaaatcc tacaggttaa aagtagactg aattgtttac atttaaatac
    10681 tgtagtaata tgaaaaagtc aagatttacc gagacacaga ttaccggagc cttaaaggcg
    10741 tatgaagccg gtaaggacat tcagcaaatt tgcagggaga tgaatatcaa ccgggcaaca
    10801 ttttataatt ggaagaagaa gtattcaggc atggatgctg atctgttgcg gcaatttaaa
    10861 gagcttcagc gggagaatac agagttgaag cgtatgtatg cagacctgag cctggatcac
    10921 aacatcctta aaaaggttat tgaaaaaaag ctttagggcc ctgtcaaaag cgtttggtgg
    10981 ctgcggagat tgttcaggaa gaacaggtga gcattgtcag ggcctgccgg attgttcaac
    11041 tggaccggtc actgttttac tatcggtcaa tgaaagatga cagtgaagtg gaggccaagc
    11101 tgaccagcta tttggataat aaagttcttt gcaaccgggg atgtcctgag tattataagc
    11161 gtatgcggcg tgaaggtttg atgtggaatc acaaacggat agaaagggtg tatcggaaga
    11221 tgggtttaag caagcgacgt aaaagaaaac gcaggctgcc taacccggag aagcggccat
    11281 tggttcaacc ggtattgatg aatgaaacat ggagcatgga ctttgtagaa gacaggcttg
    11341 aaaacggcag gaagatcaga acactcaata tcctggacga ttttaaccgt gaggccctgg
    11401 cgatggaggt atccttttcc tttcctgcag agcaggtaat agaagtaata aatcaatgca
    11461 ttgaatggag gggtaaacct aaaagaatca ggtcagacaa tggtacagag ttcgtagcta
    11521 tcaaaacaca acagttcttt tccaataaag cgattgagca tattcctatt cagaaaggga
    11581 agcccatgca aaacggatat gtggagcggt tcaacagaag ttacagggaa ggtgtactag
    11641 atgcatttat actgacctct attgaacaag ccagggaaga aacaactgtc tggatggaag
    11701 actataaccg taatcatcca catgaaagcc ttggagataa acctccgctt gaatatggat
    11761 gaaaagattt ttagtaatca cagggaaacg gattaattta ataatttaac cctgtaggaa
    11821 aaagggggga cttacaaaat gtaataagga tgaaaatacc gatttgcttt tccagctttt
    11881 tttgaagcag gttgatattg ataatgacca gccgcatcag gtgggtaagc accaggccca
    11941 gcgccacgaa aatacccagg cgtcccaaaa cctctatgac ttgttttttg gtgttgagct
    12001 gatcaaatga gtaagcaaag aaaatattga tcagggtaaa tcca
//
LOCUS       DAORLS010000061        11937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2866_length_11937_cov_2.463979, whole genome shotgun sequence.
ACCESSION   DAORLS010000061 DAORLS010000000
VERSION     DAORLS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11937
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_11937_cov_2.463979"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PK977_06535"
     CDS             complement(<1..393)
                     /locus_tag="PK977_06535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF17804.1"
                     /translation="MPNINDIFFDGHYKEIWKTIIPDELTVKEVDFMIPYFSLQPGSK
                     VLDLMCGYGRHAIGLARKGVTVTAVDNLADYINEIQQTATTENLPVKAVQSGVLDFST
                     EEQFDLVICMGNSLNFFNAADTVSILKKA"
     assembly_gap    439..448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(501..1157)
                     /locus_tag="PK977_06540"
     CDS             complement(501..1157)
                     /locus_tag="PK977_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17805.1"
                     /translation="MTAQQIKVAIADDHKIFRDGIRMALKDKDYLKIIWEAEDGKDLM
                     HKLQLKQPDVILMDIRMPEMDGINAISLIRKEYEAVKIIVLTMYDDQEMITKMMEMGA
                     NAYLTKTTDPEEIYQAILTCMNDDFYFNELVNKAVLLKLQHKKTVRQFYPNPVKFSEK
                     ELRILKLIADDKTTEEISKEVFLSPRTIETIRQNMKQKVGAKTIAGLVMYATRNKLLE
                     "
     gene            2106..2798
                     /locus_tag="PK977_06545"
     CDS             2106..2798
                     /locus_tag="PK977_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17806.1"
                     /translation="MDKIQIIEKLFYNIVTYSYLLLPLCFFWCGNKKKDLIPIVLALY
                     SVICFFFLEYYDTFPKEIRKYLKTFYTFFEYSIFTIVIWYSLKSKKARQFILMASLAF
                     LSFQLIYLFNGKVKRLDTVPVGIETILLITYVVYFFYQYYKNISTSYIYNHYIFWISV
                     GILIYLGGSFFFYILINHLSEEQINTFGTLTYLAEIIKNILFVIALVVYVRFPVENNE
                     KKQTHIPYLDMI"
     gene            2822..3649
                     /locus_tag="PK977_06550"
     CDS             2822..3649
                     /locus_tag="PK977_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF17807.1"
                     /translation="MFFLQATNSSGVSTVLFLGTVGMVVLVVALIIFLIMHQRRVLRF
                     HKKIQQMEAEQQKILLNASIKLQEEERQRLAADLHDDAGPLLATARLYLNENLLTQDK
                     ATQLQSIFQARQIIDDTIQLVRNISHSLMPPTLKNFGLESAVNDLFQKISGSGTINAS
                     SRFHEYKERLKEEKELIVFRVVQELINNILKHSNSSFIHLTQNVHADKFYLRIHHDGR
                     GIVQSDFDKLNKSNVGLGLKNISSRLRVVQGSINFEKDISQTYYKVTIELPKDEPYE"
     gene            3760..4410
                     /locus_tag="PK977_06555"
     CDS             3760..4410
                     /locus_tag="PK977_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17808.1"
                     /translation="MSVIKVAIADDHKIFRKGVILSLRPFSNIKFVLEAENGDELLNG
                     LPAAEPDVVLMDLRMPGKDGIEATKAISKQFPQIKVLVLSMYEDERFVYHLMENGANG
                     YLLKNAEPNEIRRAIMEVYEKGYYLNNFVNRILLKKSHSRQKVVPSLNNEITLSDKER
                     DVLRFICMEFTAAEIAQKMEISPRTVEAIKDRLMERFGSKNTAGLVFFAVKNNLID"
     gene            complement(4481..5137)
                     /locus_tag="PK977_06560"
     CDS             complement(4481..5137)
                     /locus_tag="PK977_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HRF17809.1"
                     /translation="MALTKEQIAERIARELRDGYYVNLGIGIPTLVANYIPEGMDVVL
                     QSENGLLGMGPFPFEGEEDPDLINAGKQTITTVPGSVFFDSAMSFGMIRAGKVDLTVL
                     GAMEVSENGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMMHTNPKGESKLLPQCTL
                     PITGVRCVKKIVSDLAVLDITPQGFKLLERAPGVSVDEIRAKTAGKLIIEGDVPEIVF
                     "
     gene            complement(5186..5881)
                     /locus_tag="PK977_06565"
     CDS             complement(5186..5881)
                     /locus_tag="PK977_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRF17810.1"
                     /translation="MNKVVSGAEAAIHDISDGSVIMLGGFGLCGIPENCINALVKKGV
                     KNLTCISNNAGVDDFGIGLMLQQKQVKKMISSYVGENAEFERQLLSGELEVELIPQGT
                     LATRCLAAGYGMPAIFTPAGAGTEVGTGKEVRNFNGKDYLLEYAFDADFAIVKAWKGD
                     TAGNLVFKETARNFNPLMAMAGKITIAEVEELVPAGALDPDYIHTPGIYVHRIFQGSN
                     YEKRIEQRTVRKR"
     gene            complement(5937..6383)
                     /locus_tag="PK977_06570"
     CDS             complement(5937..6383)
                     /locus_tag="PK977_06570"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRF17811.1"
                     /translation="MRQILLVVSVFALLFAACKSKRSNSVIPPDKMELVLRDMMRADK
                     FLADYVLNKDTTKKIDSESVKLYQQIFSIHQISGEQFQKSFSYYKEHPDQLRAIMDSL
                     SRPVSAAPTEMVQPLPAPQPQSAADTTQNKADTNQKPKIKKVMAID"
     gene            complement(6380..7297)
                     /locus_tag="PK977_06575"
     CDS             complement(6380..7297)
                     /locus_tag="PK977_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRF17812.1"
                     /translation="MLKRILIVILIVSLTITSHAQEIQARFSVIASKVSTQVDRKVFQ
                     TLQTALTNFLNSRRWTNDVFQANEKIQCSFLLTIDQDLGNNVYKGKLTVQAARPVFNT
                     SYVSPIINYIDDNVVFKYIEFQPVEFNENRVQGNDPLVANLTAILAYYVNIILGFDYD
                     SFTQRGGDTYFQKAWNIVNNAPESRDVTGWKSFESLRNRYWLAENLNTSRYALVHDAI
                     YAYYRTGMDLFYENEDAGRSGILNCLNYLNTVNTENPNSMIMQFFFQGKSTELVKVFS
                     KATPDTKTRARELLSRLDITNSQKYKELK"
     gene            complement(7290..8507)
                     /gene="coaBC"
                     /locus_tag="PK977_06580"
     CDS             complement(7290..8507)
                     /gene="coaBC"
                     /locus_tag="PK977_06580"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRF17813.1"
                     /translation="MVFLHSMLSCKKILLGITGSIAAYKSVYLVRQLVKEGADVKVIM
                     TPAAKDFVSTLTLSTLSKNPVLVDLFDEQSWSNHVMLGRWADVIVIAPLSCNTLAKMA
                     NGQCDNLLLATYLSATCPVVVAPAMDEDMWHHPSTKHNINRLTEYGNHVIPVEKGDLA
                     SGLNGEGRMAEPETIVEFLRTRFFLPERLAGKKALVTAGPTYEPIDPVRFIGNHSSGK
                     MGLAIARELATRGADVSLVMGPTQLPLNVERGITIKRVTTAQQMFDACVEEYADTDIT
                     VMAAAVADYTPETVASQKIKKKDDKLSLSLRKTNDILKHLGEQKKKGQVLAGFALETT
                     NERQHALEKLQKKNADLIILNSLNDAGAGFGHDTNKITIFDKSGKEFSFDNKSKTAAA
                     KDIVDTIISLYYA"
     gene            complement(8607..8948)
                     /locus_tag="PK977_06585"
     CDS             complement(8607..8948)
                     /locus_tag="PK977_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRF17814.1"
                     /translation="MSKLRRQLSAGITNNVETRNLDDIKAKTGNLYESIAIVGKRANQ
                     INISLKEELHNKLEEFASHTDSLEEIHENKEQIEISRAYERMPNPALLATQEFLDDKV
                     YFRKSEDELFS"
     gene            complement(8945..9784)
                     /gene="bamD"
                     /locus_tag="PK977_06590"
     CDS             complement(8945..9784)
                     /gene="bamD"
                     /locus_tag="PK977_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRF17815.1"
                     /translation="MRFSWLIIAVFILGSCSGGKLFSKKSSSKNTSKSISKILKNPDP
                     AYKLRIAEQFFVKKKYSKAQQVYEDVMPYYKTGKEFEDIYYKYAYCAYYQGDYMNSEN
                     LFKSYLEIFPNSTKAEEVDYMRAYSYYKQSPKPELDQTSTVKAMGMMQTFINTHPGST
                     RNKEANEIIDICRAKLETKDYKSALLYYDIGQFRAAGVAFTTLLNTYPESLRADEYKL
                     MIIKAYYKFAEMSIEEKKVERFEQVVSECYDFTDRFPESKFKKEADQFLALSQNNIKN
                     YKL"
     gene            complement(9954..>10253)
                     /locus_tag="PK977_06595"
     CDS             complement(9954..>10253)
                     /locus_tag="PK977_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17816.1"
                     /translation="DDAKAVLATVAAKLRNNPGCNVVVVGYCSSNKKEQQLSWDHVNA
                     VINYMVDKEGISADRFIFNYGQEGGDCNTVDLRAAAEGEQGPNRVEPPHPNLRKN"
     assembly_gap    10254..10271
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10272..11681)
                     /locus_tag="PK977_06600"
     CDS             complement(<10272..11681)
                     /locus_tag="PK977_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17817.1"
                     /translation="MASKKFLLLTGMFCLLASYGFSQRVGASYDVKDSSVVPSKRMPQ
                     HTEFLNGTYNFPAKPRNQWEIGIKAGLFQVSGDIPAKFLSPGFGAHVRKAFGYIFSMR
                     LEYMYGIGKGYSFNRSYNYAKNPAWAQNGSAATSYSAPVLVGNPTGAAIISSQSAYYG
                     LPANTPIEYVYYNYKTNVQDLSLQGVVTLNNIRFHKSKTGFNFYGFAGVGGTVYDTKI
                     NALNEGVNGNQKYNFASITPSDEYKKRRDTYKALKNLLDDTYETPAENQGARRPKLFG
                     KTFKPSGTVGAGIAFKLNNRINIALEDRWTFIKDDLLDGQRWQEHATGDAVLTRDFDS
                     YNYLSLGLNINLGAKSVEPLWWLNPLDYAYSEIRNPRLMRLPKPVLPDSDGDGVTDQF
                     DQEQTPAGCPVDSHGVSKDTDGDGVPDCKDKELITPTYCQPVDADGVGKCPCPEGCVG
                     VAPPEKTCAEKLGPLPSVSF"
BASE COUNT         3341 a         2655 c         2258 g         3655 t
ORIGIN      
        1 tgcctttttt aaaatggata ctgtatcagc cgcattgaag aaattcagac tgttgcccat
       61 gcagattacc aggtcaaatt gttcttccgt agaaaaatca agcacacctg actgcacagc
      121 ttttaccggc aggttttctg tagtagccgt ttgctgtatt tcattgatat aatctgcaag
      181 attatctacc gctgttactg ttactccttt tcgggcaagg ccaattgcat gacgtccgta
      241 gccacacatc aggtccagca ccttactgcc cggctgcagg ctgaagtaag gtatcataaa
      301 atcaacttcc ttcaccgtca gttcatcagg tatgatcgtt ttccagatct ctttatagtg
      361 accgtcaaaa aaaatatcgt tgatatttgg catcgcttga gtttaatgat tgatctctct
      421 gcaagaattg catcaagtnn nnnnnnnnag ccttctgcaa gctcaggctg gtaacttttt
      481 attccgcagc gttccctggc ctattccagc aacttattcc tggtagcata cattaccaaa
      541 cccgcaatgg tttttgctcc cactttctgt ttcatattct ggcggatggt ttcaatggtg
      601 cgggggctaa gaaatacttc tttagagatt tcttccgtag tcttatcgtc cgcaatcagt
      661 tttaagattc ttaattcctt ttctgaaaac ttaacagggt tcggatagaa ttgccgtacc
      721 gttttcttat gctgcaactt cagcaaaacc gctttattca ccagttcatt gaaataaaaa
      781 tcatcattca tgcaggtgag tatggcctgg taaatttctt cggggtcagt cgtcttggtc
      841 aggtaggcat tggcgcccat ctccatcatc ttagtgatca tctcctggtc atcgtacatg
      901 gtcagcacta tgattttcac ggcctcgtat tccttacgga tgaggctgat ggcattgatg
      961 ccgtccattt ccggcatgcg gatatccatc aggataacat ccggttgctt tagctgaagt
     1021 ttgtgcatca ggtcttttcc gtcttcagct tcccaaataa ttttcaggta atctttgtct
     1081 ttcagcgcca tgcggatacc atcacgaaag atcttatgat catcggcaat cgctacttta
     1141 atttgctggg cggtcatggg tattggtttg cagttaagtt tttgggtatc gaagtcgagt
     1201 aaaactacgc aaatttggta gaaatacgta aaattagttt tgcatcactc aaaacaaatt
     1261 ttatcatcac atctcacaaa tattaatagc cttttgtgaa agagaaggaa gaaaacaaca
     1321 ctggaataaa aacaaggtct tacagggaga gaaaatccca ttaccgtaaa ttttcatccc
     1381 ccctgatagt agattttcat tttcctatga ggcagttacg atgctgtatg taattcctga
     1441 aaacccaaaa agccgtgcat ttgctcttgt aaggcttttt gccaggggct acttttgacc
     1501 cagttgattg acagaagata gagccccgcg gtcgtccaat gtcctgaact aaccgtcctg
     1561 aatactattt ccgataccaa tgaatgccat agctaaatcc aatgtcaatc aacttctttc
     1621 cgggcgaagc gaaggaacct ccttcaaatt tgcagagtcg cagtctcttt aaaactgcat
     1681 cggtttaaag caactttaaa ccttaaactt ccaacccgaa acacctcttt cttttccaca
     1741 gctgttgata accggaaata tccccgggaa gtttactatt tttacaaccg taattttacg
     1801 tattttacta agaaaactta ctatttttaa aatcgtaatc acactattta aagtctataa
     1861 aatcctttgt tacagagcct tttaggtggt tttaggggta tttatttagc cggcaccaca
     1921 ttgtatccca tccggcgaat agcaattttt acacctcgag ttaattcgaa taaggcttta
     1981 aaaacgttac tgttatgaaa aaagttagaa gagcatggga cgtagatgtc ctgattggat
     2041 gggaagaata atcattcaat cacttaattt ctactgttag agaagtttaa aaagtttttc
     2101 tatttttgga caaaatccaa attatagaaa agctttttta taatatagtc acttattctt
     2161 atctcctttt acccctttgt tttttttggt gtggaaacaa aaagaaagac ttaattccaa
     2221 ttgtcttggc tttatatagt gtcatttgct ttttctttct tgaatattat gacacatttc
     2281 caaaagagat tagaaagtat ctaaaaacct tctatacttt ttttgagtac agcatattca
     2341 caattgtaat atggtactca ttaaagagca aaaaggccag acagtttatt ctaatggcat
     2401 cacttgcttt tttaagtttc caactcattt atctttttaa cggaaaagtt aaaagattag
     2461 atacagtacc tgtcggtatt gaaacaatac tcctgattac ttacgttgtt tacttttttt
     2521 accaatacta caagaacatt agcacctcat acatttacaa ccactatatt ttttggatct
     2581 cagtaggtat cctgatttac ttaggaggat cttttttctt ttatatacta ataaaccatt
     2641 taagtgagga acaaatcaat acttttggaa cacttacata tttggcagaa ataattaaga
     2701 acattctatt tgtaattgct ttagttgttt atgttaggtt tcctgttgaa aacaatgaaa
     2761 aaaaacaaac acatatccct tatttagata tgatttaacc catcgcaacc actaacccac
     2821 tatgttcttt ttacaggcca ctaacagttc cggagtttcc acagtgctct tcctgggtac
     2881 cgtaggcatg gtagtgctgg tggtagccct tatcatcttc ctcatcatgc accagcgccg
     2941 ggtgctgcgt tttcataaaa aaatacagca aatggaagcc gagcagcaaa aaatactgct
     3001 gaatgcctcc atcaaactgc aggaagaaga aagacaacgc ctggccgccg acctgcatga
     3061 tgatgccggc ccgctgctgg ccaccgcccg tctgtacctg aatgaaaacc tgttaaccca
     3121 ggataaggcc acccagctgc aatccatctt ccaggcccgc cagatcattg atgataccat
     3181 ccagctggtg cgtaacataa gccacagcct gatgccgcct accctgaaga atttcgggct
     3241 ggagtcggcg gtgaatgacc tgttccagaa aatcagtggc tcaggcacta tcaatgccag
     3301 cagccgtttt catgagtata aagaaagact gaaagaagaa aaggaactga tcgttttccg
     3361 ggttgtgcag gaactgatca ataatattct caagcacagt aattcaagtt tcatccacct
     3421 cacccagaat gtacatgccg ataaatttta cctgcgtatc caccacgacg gcaggggtat
     3481 tgtacaatct gattttgata aactgaacaa gagcaatgta ggccttgggc tcaagaacat
     3541 cagcagccgc ctgcgggtag tgcagggaag tatcaatttt gaaaaagata tttcccagac
     3601 ctattacaaa gtcaccatag aattgccgaa agacgaaccg tatgagtagt taataggttg
     3661 aatggttaac gggttaaaaa ggactacctg ctaccgttaa taacctgtgt caccttatta
     3721 acttgtaaac ttgttaactt taccgcatta ttgataacta tgagtgtcat taaggtagct
     3781 attgcagacg atcacaaaat cttcaggaaa ggagtaattc tttcacttcg tcctttttcc
     3841 aatatcaagt ttgtactgga agcagaaaat ggtgatgagc tgttaaacgg actacctgct
     3901 gccgagcccg atgtggtgct gatggacctg cgtatgcccg gtaaggatgg catagaagct
     3961 accaaagcta tcagcaaaca atttccgcag ataaaagtgc tggtgctgag tatgtacgaa
     4021 gatgaacggt ttgtgtacca cctgatggaa aacggcgcca atggttacct gctgaaaaat
     4081 gcagaaccga atgagatccg ccgggcaatt atggaagtat atgagaaagg ttattacctg
     4141 aacaattttg ttaaccgcat cttgctgaaa aaatcacact cccgccagaa agtagttcct
     4201 tcacttaaca acgaaatcac cctgagcgat aaggaaagag atgtacttcg ttttatctgc
     4261 atggagttca ccgccgcaga aattgcccag aaaatggaga tcagtccccg tacagtagaa
     4321 gctatcaaag accgattgat ggagcggttt ggcagcaaga atactgcggg ccttgttttc
     4381 tttgctgtaa agaataacct gattgactga ggctggttac ggcaacttag attattacac
     4441 aattacagcc ttcgacaagc tctggctgac atacacttcc tcaaaatacg atctccggca
     4501 catccccttc tattatcaat tttccggctg ttttagccct tatttcatca acagatactc
     4561 cgggagccct ttcgagtaat ttaaagccct ggggtgttat atccagcact gcaaggtctg
     4621 aaacgatctt ctttacacaa cgtacacctg ttatgggtag tgtacactga ggcagtaatt
     4681 tcgactcccc tttggggttg gtatgcatca tggccacaat aatatttttg gcgctggcca
     4741 ccaggtccat ggcaccgccc attcctttta ccattttgcc tggtattttc cagttggcaa
     4801 tatctccgtt ctcacttacc tccattgcgc ctaacactgt caggtcaact ttcccggccc
     4861 ttatcattcc aaaactcatg gctgaatcaa aaaagacgga tcccggcacg gtagtaatcg
     4921 tttgcttacc ggcattgatc aaatcgggat cttcttcgcc ttcaaaagga aaaggtccca
     4981 ttcctaataa tccgttctca ctttgcagca ctacatccat cccttcgggt atataattgg
     5041 ccactaatgt aggtatacca atgccaaggt ttacataata tccgtcacgc aattctcttg
     5101 caattctttc tgctatttgt tcttttgtta aagccatttc aatttgaaga tttgagaatg
     5161 aaataatttg aagatatcgc tgcacttatc tcttccttac tgttcgctgc tcaatccttt
     5221 tttcataatt gcttccctgg aaaatacggt gcacataaat gcccggtgta tgtatgtaat
     5281 caggatccag tgctcctgca ggaaccagct cttctacctc tgctatcgtt atttttccgg
     5341 ccatagccat cagcggattg aaattgcggg ctgtttcttt gaataccagg tttcctgcag
     5401 tatctccttt ccatgctttc acaatagcaa aatcagcatc gaatgcatat tcgagcaagt
     5461 aatcttttcc attgaaattt cttacttcct taccggtacc cacttccgtg ccggcaccag
     5521 ccggggtaaa gatggcaggc ataccatagc cggcagccaa acagcgggtg gccaatgtac
     5581 cttgcggaat aagctccact tccaattcac cgcttagtaa ctgtctttca aactctgcat
     5641 tctctcccac ataagacgag atcatttttt ttacttgttt ttgctgcagc atcagcccaa
     5701 taccaaaatc atccaccccg gcattattgg aaatacaggt tagatttttt accccctttt
     5761 ttaccaatgc attgatacag ttttccggta taccgcataa tccaaagcct cccagcataa
     5821 tcaccgatcc atcgctgata tcatgaatag ctgcttcagc tccgcttact actttgttca
     5881 tgaaagaata tttaaagtaa aaatacagaa gtcgggctga atggctcagc aactgtttaa
     5941 tcgatagcca tcaccttttt aatctttggc ttttgattgg tgtccgcttt gttttgagtg
     6001 gtatcggcag ccgattgcgg ctgcggtgca ggaaggggtt gtaccatttc tgttggagca
     6061 gcagatacag gcctgctcag ggaatccata attgcccgta actgatccgg gtgttctttg
     6121 tagtaagaaa aacttttttg aaactgttcg ccggatattt ggtgaataga aaatatttgc
     6181 tggtataatt tgacactctc cgaatcgatt tttttggttg tgtccttgtt caggacataa
     6241 tcggccagga atttatctgc ccgcatcata tcccggagca ctaattccat tttatccgga
     6301 ggaattacac tattacttcg tttgctttta catgctgcaa ataagagtgc aaaaactgaa
     6361 actactaata agatttgcct catttcagtt ctttgtattt ctgcgaattg gtaatgtcaa
     6421 gccggctcag taattctctg gctcttgtct ttgtatccgg tgtggccttg ctgaaaacct
     6481 tgaccagttc agtgcttttt ccctggaaaa aaaattgcat gatcattgag ttgggatttt
     6541 ctgtattgac agtattcaaa taattaaggc aattgagaat accgctacgt cccgcatctt
     6601 cattttcata gaaaaggtcc ataccggttc ggtaataagc atagatcgca tcatgtacca
     6661 gcgcatatcg gctggtgttc aggttttcgg ccagccaata acggttgcgt aaactctcaa
     6721 aagatttcca tcctgtaaca tcacggcttt ccggtgcatt gtttacaata ttccatgcct
     6781 tctgaaaata agtatcccct cccctttgag taaaagagtc gtaatcaaat ccaagtatga
     6841 tattgacgta ataagccagt atagctgtaa ggttggctac cagcgggtca ttgccctgta
     6901 ctctgttttc attaaactct accggctgaa attcgatata cttaaaaact acattatcat
     6961 caatgtaatt gataataggt gaaacatagc ttgtattaaa aacaggcctg gctgcctgta
     7021 cagttagctt acccttgtac acattattac ccaggtcctg gtctatcgtt agcagaaagc
     7081 tgcattgaat cttttcgttg gcctggaaaa catcgttggt ccagcgacgg ctgttgagga
     7141 aatttgtcaa agccgtttgc agggtttgaa acacttttct atctacctgt gtacttactt
     7201 tactggctat aacagagaac cgtgcctgta tttcctgggc atgcgaagta atggtaagcg
     7261 aaactatcag gattacgatc aaaattcttt taagcataat acaagctgat tatagtatct
     7321 acaatgtctt ttgctgctgc ggttttggat ttattatcaa agctgaattc tttaccgctt
     7381 ttatcgaaaa tagtaatctt attggtatca tgtccaaaac cggcaccggc atcatttagt
     7441 gaattcaaaa tgatcaaatc cgcattcttc ttctggagct tttccagtgc atgttgccgc
     7501 tcattggttg tctccaaagc aaaacccgcc aaaacctgcc cttttttctt ttgttctcca
     7561 aggtgtttta aaatatcatt tgttttcctc aggctcaaac tcaacttatc atctttcttc
     7621 tttattttct ggctggctac cgtttcaggt gtataatctg ctactgcagc agccattact
     7681 gtaatatctg tatctgcata ctcttccacg caggcatcaa acatttgttg tgctgtggtt
     7741 actcttttta ttgtaatccc cctttccaca tttaaaggca gttgtgtcgg tcccattacc
     7801 aggctaacat cagcaccccg ggtggccaat tctcttgcta ttgccagtcc catttttccg
     7861 gaagagtgat tgccgataaa acgaacagga tcgatgggtt cgtatgtagg ccctgcggta
     7921 actaatgctt tcttaccagc taatctttca ggcaaaaaaa aacgggtcct taaaaattca
     7981 actatagttt ctggctcagc cattcttcct tcaccattta gtccgctggc gaggtctccc
     8041 ttttcaacgg gaatgacatg attaccatat tcagttaacc tgttgatgtt atgctttgtg
     8101 gacgggtggt gccacatatc ctcgtccata gccggggcta ctaccactgg acaggtagcc
     8161 gacaaatagg tagccaaaag caggttatca cactggccat tcgccatttt tgccaatgta
     8221 ttacagctca aaggtgcaat aacgattaca tctgcccaac gacccagcat tacatgatta
     8281 gaccagcttt gctcatcaaa cagatcgacc agtaccggat ttttgctgag cgtcgacaaa
     8341 gtaagagtgg atacaaaatc ttttgcagcc ggggtcatga ttacttttac atcagccccc
     8401 tctttcacca actggcgaac aagataaact gacttgtagg cagcaatact gcctgttatg
     8461 cccaacaata tcttcttgca gcttaacata gagtgtaaaa ataccattta cctttttaag
     8521 gacgaagcat ttggggcaaa tccctgttaa atcaaaaaag cgatggacat ttccatcgct
     8581 ttactaatat gatctgttag caaaaatcag ctgaatagct cgtcctcgct tttgcggaaa
     8641 tacactttgt catcgagaaa ttcctgtgtg gccagcaaag caggattagg catcctttca
     8701 taagctcttg agatttcaat ctgctcttta ttttcatgaa tttcttcgag gctgtctgta
     8761 tgactggcaa attcttccag tttgttgtgc aactcttcct tcagagagat attgatctgg
     8821 ttggcccttt taccaacaat ggcaatcgac tcgtacaaat tgcctgtctt tgccttgata
     8881 tcatcaaggt tgcgggtttc aacattattg gtaatgcctg cacttaactg gcgccttaac
     8941 ttgctcataa tttataattt ttgatattgt tttgtgataa tgccaaaaac tggtcagctt
     9001 cttttttgaa tttactttcc ggaaaacggt cggtaaaatc ataacattca gaaacaactt
     9061 gctcaaacct ttccactttc ttctcttcaa tactcatctc agcaaactta taataggcct
     9121 ttatgatcat tagtttatac tcatctgccc tcaacgattc aggataggta ttcagcagcg
     9181 tagtgaaagc tacaccagct gccctgaact gtccaatgtc gtagtataac aaagctgatt
     9241 tgtagtcctt ggtttcaagc ttagccctgc aaatatcaat gatctcattg gcctccttat
     9301 tcctggtaga tccgggatgg gtattgataa atgtctgcat catacccatt gcttttacag
     9361 tgcttgtctg gtcaagttcc ggcttaggag attgcttgta ataggaatat gcccgcatat
     9421 aatccacttc ttcggccttt gtactatttg gaaaaatttc caggtagctt ttaaaaaggt
     9481 tctcagaatt catgtaatct ccctggtaat aggcacagta agcatattta taataaatat
     9541 cctcaaactc cttacctgtt ttgtaataag gcattacatc ctcatatacc tgctgtgcct
     9601 tactatactt tttctttaca aagaactgtt cggcaatacg caatttgtaa gccgggtcag
     9661 gattctttaa aatcttgctg atactctttg aagtgttttt ggaactgctt tttttagaaa
     9721 acaactttcc gccactacaa cttcccaaaa taaatacggc aatgattaac cagctaaatc
     9781 tcataaaggt gtgcaaagtt aacagatagc tacaaatctt actcaaaaaa gattagatgc
     9841 aaaaccgaca gcgttaagat tttggtaaag gattgcttat gaacacaata caagaaaaga
     9901 ccccgatcca gctaaaccgg aacggggtcg attattaaaa gacaaactaa atcttagttc
     9961 ttacgcaggt tcgggtgagg aggctcaacc ctgttaggac cttgctcacc ttcagcagca
    10021 gcacggaggt caactgtatt gcaatcgcca ccttcctggc catagttgaa gataaagcgg
    10081 tcagcgctga taccttcttt atcaaccata taattgataa ctgcattcac atgatcccag
    10141 ctaagctgct gttctttctt atttgaagag caatagccta ctaccactac attacagcct
    10201 gggttgttac gcagtttggc agctactgta gccaatacag cttttgcatc atcnnnnnnn
    10261 nnnnnnnnnn ngaagctaac gcttggcaga ggaccgagtt tttcagcgca ggttttctcc
    10321 ggaggagcaa ctcccacaca accttctggg caaggacact taccaacacc atcagcatca
    10381 acaggctggc aataagtagg tgtgataagt tctttatcct tacaatcagg aacgccatca
    10441 ccatctgtat cttttgaaac accatggctg tcaacagggc aacctgcagg agtttgctcc
    10501 tggtcgaact gatctgttac accatcacca tcactatcag gcaatacagg cttaggcaga
    10561 cgcattaaac gtgggttgcg aatctcgctg tatgcataat ccaatggatt cagccaccaa
    10621 agaggttcaa ccgacttagc acctaaattg atattaagac caaggctcag gtagttataa
    10681 gagtcaaaat cacgggtaag tacagcatca cctgttgcat gctcttgcca acgctgacca
    10741 tccagtaaat catctttaat gaatgtccaa cgatcttcca acgcaatatt tattctgtta
    10801 ttcaatttga aagcgatacc agcacctact gtaccagaag gcttaaaggt cttgccaaac
    10861 aattttggac gacgggcacc ctggttttca gcaggtgttt cataagtatc atccagcaaa
    10921 tttttaagtg ctttgtatgt atctcttctc ttcttgtact catcagaagg agttattgaa
    10981 gcgaaattat acttctggtt accgtttaca ccttcattca aagcgttgat ttttgtatca
    11041 taaacagtac cacctacacc agcaaatccg tagaaattaa atccggtttt tgatttgtga
    11101 aagcgaatat tgtttaaggt tacaacgccc tgtaaagaca ggtcctgaac gttagtttta
    11161 taattgtaat aaacatactc tattggcgta ttggcaggaa gaccatagta cgcagactgc
    11221 gatgaaataa ttgcagcccc agttggattt ccaactaaca ccggagcaga ataagatgta
    11281 gcagcagaac cgttttgagc ccatgcagga ttttttgcat agttataaga acggttaaag
    11341 ctatatccct tgccaattcc atacatatac tcaagtctca ttgagaaaat gtatccaaaa
    11401 gcctttctaa catgggcgcc aaaacccgga gaaaggaact tagcagggat atcaccactt
    11461 acctggaaca ggccggcctt aataccaatt tcccactggt tacgtggctt tgccgggaag
    11521 ttgtatgtac cgttgagaaa ctcggtatgc tgaggcatcc tcttgctggg aaccacagag
    11581 gaatctttta cgtcgtaact ggctccaact ctttgagaaa agccataaga cgctaacaga
    11641 cagaacatgc ctgtcaacaa taaaaatttt ttgcttgcca taactaagga ttaagatttg
    11701 tgaacaatgt cctaaatatt cgcaaaaata gaaattgagg agcaattacc aaatttttat
    11761 gacaattatt tccttccact ttcctattat tttcaattac ttagtcagtt tttgaggtca
    11821 ttttgctgca taatgttgca attatttttt tgctcgtaaa ttgccccctt tcctttatga
    11881 acttacccac ccaactgata agtaatgaac tggatgcctt ccaggaacgg tttaaag
//
LOCUS       DAORLS010000062        11899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2877_length_11899_cov_1.914218, whole genome shotgun sequence.
ACCESSION   DAORLS010000062 DAORLS010000000
VERSION     DAORLS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11899
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2877_length_11899_cov_1.914218"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PK977_06605"
     CDS             complement(<1..1111)
                     /locus_tag="PK977_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF17818.1"
                     /translation="MNKKLKWTLIVAGGLAVVFVIAKAVGGSGSSTEKVATEKAAKRT
                     IIETVNASGKIYPEVEVKISPDISGEVTELNVEEGDSVKKGQILARIYADIYALQRDE
                     AASRVNQSSATVDNSQAALESLRANMELAQQTFDRYKKLYDDKVISKAELEQYETTLR
                     SAKANYTAAQQNIKSLKAGVQSTQVGLNRANKDLSRTVLTAPMDGVISSLKIKKGERV
                     AGNSFNIGTEMMTVADMAVLEVRVDVGENDIVKINIGDSADVEVDAYNNRKFKGVVTK
                     IASSTKTSAASLTSNDVTNYEVRIRLDKSSYQDLAGKTFPFRPGMNASADIKTKRVDN
                     VLAVPIGAVGARVKGSDKSMADKQKEEKEKQGADAD"
     gene            complement(1125..>2310)
                     /locus_tag="PK977_06610"
     CDS             complement(1125..>2310)
                     /locus_tag="PK977_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF17819.1"
                     /translation="NFFNLGMQLQSQVTIFNWFSRKNTIEASRLSWEADKEQLKKSQD
                     DIALNVAVAYLQILLAKEQVNIAKVQVAQTSTQLETTRKRVETGVLPELNAAELEAQL
                     ARDSSSLVSAEASVQQFLLQMKALLNLDAGTVFDIETPPVSLIPIEPLADLQPDAVYA
                     LAIGTMPQQKVNLLRIQSAQKSVAAAKAALYPTISAFGSLSTNAISFKKPIYEQILNG
                     YAPTGARANAGGGTFYPVEVPDFIDGSNVVGYFKPGSFPRQFSNNFGQSIGIGLNVPI
                     FNSRSSRTAWERSKLTVQQWQLQKEQGDMQLKQDIYKAYTDATAAIQKFNADKKAVQT
                     AEKAYEFANKRYALNLLSTYDLVNSQNNVLRAKIQALYSQFDYVFKMKLLEFYRGQGL
                     KL"
     assembly_gap    2311..2505
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2646..3806)
                     /locus_tag="PK977_06615"
     CDS             complement(2646..3806)
                     /locus_tag="PK977_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRF17820.1"
                     /translation="MDISQIINELGEDRDHYFNAIAPPIIQTSNFAFKNVAALSKAFE
                     DEMGGYLYSRGLNPTVDILRKKLAALDGAEDCLVFNNGAAAIFAGIFANIKSGDHIVS
                     VKAPYTWVQRMFDVILPRFNVSVTYIDGTSIANWKNATKENTSFYYLESPNSWDFAIQ
                     PIREVAELARSKGIVTLIDNSYCTPLYQKPIEMGIDMVMQTATKYIGGHSDTLGGVLC
                     GSHTMIKKIFDSEYLNIGSGIQPFNAWLMIRGLRTLPARIERITKSTATVVSFMKQHP
                     KVESLIFPLDESFPQFALAKDQMKGACGLFTFTLKTGKRDSIVQFCESLKHIMMAVSW
                     GGHESLVIPKCAGIPMAEFDTSNREHRYIRMYVGLEDPQYLIADLEQALNSI"
     gene            complement(3808..>4262)
                     /locus_tag="PK977_06620"
     CDS             complement(3808..>4262)
                     /locus_tag="PK977_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRF17821.1"
                     /translation="VLLLSNPRVMVAMSEDKILPAAFQQRNSRGALTLALTLFAVIAA
                     LIVFWAKEFDTLLSFTIFLDCFGMVLSAGSIFIIRKRTANLNGTGIYSMRFYPLLPLV
                     FIAAYSFVAISLLINETKLSLIGLGVLAGFILIYFAVKALRPGHQQNS"
     assembly_gap    4263..4310
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4311..5176)
                     /locus_tag="PK977_06625"
     CDS             complement(<4311..5176)
                     /locus_tag="PK977_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF17822.1"
                     /translation="MTSRNKLGLFDLTMIVVSLVIGMGIFRTPANVAAASPTPLIFFI
                     AWAAGGIVALCGALTYAEIGSRLPVMGGYYKVFSYAYHPSIAFAINCIILVSNAASGA
                     AVALVGSEYITGVILPHATDKAYIQTIHITIALAAIAIFYGVNMLGLRLSAKTQTVLT
                     IIKIGLVLLLITPLFFASGSDNGVSSLISPSIDPGFKEYIKAFGVGLVAVSFTYGGYQ
                     QTINFGAEVEKPNRNIPRGIFMGIFIIITLYLTINYAYVEVIGFEELKTSKNIAAIMA
                     SKIFGVQAERVLS"
     assembly_gap    5409..5440
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5441..>7133
                     /locus_tag="PK977_06630"
     CDS             <5441..>7133
                     /locus_tag="PK977_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF17823.1"
                     /translation="NGLNAVAGVRMEERSPGSYRINIRGSSLRSPFGVRNIKVYWNGI
                     PVTDPGGNTYFNQFAWNNFSSIEITKGPSGSMYGAGTGGLILLNNWQPGANLEYNTGS
                     YNLHNIFTSAKFGNKDNRSYISYAHTQTDGYRVQTNMRRDNFSWQTKWSKEKYELTAS
                     VLFTDMYYQTPGALTLTEFSNNPKAARPAGGGFPSAVNAKAAIFQKNFLAGINNYFII
                     SPSIKNNTIIYGSYAQVKNSAIRNYERRTEPSFGGRSVFSWRKSNPQSDWNIIAGAEF
                     QQGYFNTQVSKNKNGNPDTLQTNDDIVTSNLSIFTQSSIDIGQSWSVIGGISITKTKL
                     ELTRLSSYPVIAQSRTYRNELAPRLSMMKKINENIHWLAAVSKGFSPPTVAEVLPSTG
                     VISTYLEAERGWNYETNFRFFLLKNRLKLELTGFYFKLNEALVQRRDAGGADFFINAG
                     DTKQKGIEFHADYSKNCFGNIISKFNIRTDFTFNHFRYGYFVKGTDDFSGNKIPSVPS
                     TVFTVLADLYSYQGIYVNANYYTASSIYLNDANTAKAEPYHLIGCQLGWKKREKGP"
     assembly_gap    7134..7190
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7316..8068)
                     /locus_tag="PK977_06635"
     CDS             complement(7316..8068)
                     /locus_tag="PK977_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF17824.1"
                     /translation="MNNSLMKVMLICVSSFLSSEPVAAQLNRPRYEIGALISGFVYQG
                     DLTPQRFGSFETTRLGFSLFGSLLLSPSFALRGNFSYGGLRGDDALYKYPVYRQQRNF
                     NFRTPVTELSVLLSWSPLADQYDSRRISPYLFTGGGISFLRIKRDWSQFNASHFGEEP
                     ELIERLGEDQAERPPRSIPVIPVGAGFRYRLTQRIGVNVESSYRLLFTDYLDGFSKAA
                     NPEKNDHYHTISAGVSYRIGQKDILGCPVVKY"
     assembly_gap    8179..8296
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      8427..8698
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8804..>10533
                     /locus_tag="PK977_06640"
     CDS             8804..>10533
                     /locus_tag="PK977_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF17825.1"
                     /translation="MKRIFFVLAAVIISTQLKAQQDTTQLEEAVITASKYERKQAETG
                     KVMTVISRQELERNSGRTLAELLNTTTGVNIVGANNNAGTNLTTSIRGSAAGNVLILI
                     DGIPVNDPSVITNYFDLNFMVTDQAERIEILKGGQSTLYGSDAVAGVINIISRQQRQN
                     GVKINGSFTGGSFNTLKQHIGLNGRNNKTSYGINYTHTSADGFSSAYDSSGSGTFDKD
                     GLNQHTLAARLGFPLSKSITADFSGTYNHYKTDLDAAAFTDERDFTAVNQNKQFAVGL
                     THKHSKGAVRLNYHYNWLRRYYLDDSSHKSSPYIDYVKSNYIGRTQYWEVFSNWRLTN
                     WEILAGMDYRLNTTDQWYWSTGAFGPYAPPKLNAEMKQLSPYASVVYKYKKLTVELGG
                     RFNKHSEYGNNFTFTFNPSWRLNSGIKAFANLYSAFKTPTLYQLFDDFAGNPGLTPEK
                     GIIAEAGAEMLKGKSFTARVVGFYRKTNDVIIYSFNPSDFTSRYINASKQINYGAELE
                     AGYNKGPWKISANYTFTSGKTTAAFDGTGTALGKDTSYFNLYRIPKHAFNLDAGFRVN
                     QSLYVSTQLR"
     assembly_gap    10534..10546
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10547..10740
                     /locus_tag="PK977_06645"
     CDS             <10547..10740
                     /locus_tag="PK977_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17826.1"
                     /translation="FIYGAPPATLDAYNTIDMYGEYKFSSLIRTFIQLKNITNKQYFD
                     ILGYNARRFNITAGINFQL"
     assembly_gap    10828..10888
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10987..11580
                     /locus_tag="PK977_06650"
     CDS             10987..11580
                     /locus_tag="PK977_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRF17827.1"
                     /translation="MQFHLQEFLTLTFTLFAVIDIVGSVPMLISIKQKMGDIHPLKVT
                     LISGALMVLFFFIGKPFLNVLGVDIKSFAVAGSFVIFILGLEMILGIEFFKAEKGTPS
                     GTVVPIAFPLIAGSGTLTTIMSLKATFERSDKNEYMILLAILVNLIVIYLVIRSLNLI
                     EKVLGKAGLMAVRKFFGVILLAIAVKVFAANAPGLIK"
     gene            11652..11723
                     /locus_tag="PK977_06655"
     tRNA            11652..11723
                     /locus_tag="PK977_06655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11685..11687,aa:Arg,seq:ccg)
BASE COUNT         3368 a         2550 c         2281 g         3176 t
ORIGIN      
        1 catctgcatc agctccctgt ttttctttct cctctttctg tttatcggcc atgctcttat
       61 cgctgccctt caccctggcc cctacagccc cgatgggaac agccagtaca ttatcaactc
      121 ttttggtttt aatatcagca ctggcattca taccgggccg gaaaggaaat gttttacccg
      181 ccaggtcctg gtatgaggac ttatccaatc gaatacgtac ttcataatta gtaacatcat
      241 ttgacgtaag cgaagcagcc gatgtcttgg tactgctggc aattttagtc accacacctt
      301 tgaactttcg gttattgtag gcatccactt ccacatctgc tgagtcgccg atatttattt
      361 ttacaatatc attctctccc acatctaccc ttacttccag aacagccata tctgcaaccg
      421 tcatcatctc tgtaccaata ttaaaactgt taccggccac cctttctcct tttttgatct
      481 tgagtgaaga aataacaccg tccattggcg ccgtaagtac agtcctgctc aggtctttgt
      541 tggcccggtt tagaccaacc tgtgtacttt gaaccccggc tttcaggctt ttgatatttt
      601 gttgtgcagc tgtgtaattg gctttggccg aacgaagcgt agtttcgtat tgctccagtt
      661 cagctttgga aatcacctta tcatcataca atttcttata ccggtcaaaa gtttgttgcg
      721 ccagctccat attagccctt aatgactcca gggcagcctg gctgttatcc acggtggcgc
      781 ttgattgatt aacccttgat gcagcctcat ctctctgcaa tgcataaata tccgcataaa
      841 tacgggccag tatctggcct tttttcacac tgtctccttc ttccacattc agctccgtca
      901 cctcccctga aatatccggg ctgatcttta cttcaacttc aggatagatt tttccgcttg
      961 cattcaccgt ttcaataatt gttcttttag cagccttttc agtagctact ttctccgtac
     1021 tgctgccact gcctcccacc gcttttgcaa taacaaaaac aactgcaaga ccccctgcta
     1081 ctatcaacgt ccattttaat ttcttattca tgatctgaaa tgatttaaag ttttaaaccc
     1141 tggccccggt aaaattccag caatttcatt ttgaagacat aatcaaattg cgaatacaag
     1201 gcctggatct ttgcccgaag cacattgttc tggctgttca ccaggtcgta ggtagacaac
     1261 aaattcagcg catatctttt attggcaaat tcgtaagcct tctcagcagt ttgtactgcc
     1321 ttcttatctg cattaaattt ttgtatcgca gcagtagcat cagtatatgc tttataaata
     1381 tcctgcttca gttgcatgtc accctgttct ttttgcaact gccattgctg aacagtgagt
     1441 tttgaacgct cccaggcagt gcgggaagaa cggctattaa atatgggcac attcaaacca
     1501 atgcctatac tctggccaaa gttgttgcta aactgccggg gaaaactgcc cggcttaaaa
     1561 tatccaacta cattactccc gtctataaag tcgggaactt caaccgggta aaatgttccc
     1621 ccacctgcat tagcccgggc ccctgttggt gcatacccgt tcagtatttg ttcataaata
     1681 ggtttcttaa atgaaatggc atttgtactt aagcttccaa aagcagatat cgtgggatat
     1741 aaagctgctt tagcagcagc aacagatttc tgtgcagatt gtatgcgcaa caggtttact
     1801 ttttgctgag gcatggtgcc aattgccaat gcataaaccg catcgggttg tagatcagcc
     1861 agtggttcga tgggtatcaa tgataccggg ggtgtttcaa tatcaaaaac tgttcctgca
     1921 tctaaattca gcagggcttt catttgcaac aaaaactgct gtaccgatgc ctcagctgat
     1981 acaagacttg aactgtccct tgccaactgg gcttccagct ccgcggcatt caattcgggc
     2041 aaaacacctg tttctactct cttcctggtc gtttccagtt gggttgaagt ttgtgctacc
     2101 tgtactttcg caatattgac ttgttctttt gccagtaata tctgcaggta ggccacagcc
     2161 acattcaggg caatatcgtc ttgtgatttt ttaagctgtt ctttatctgc ttcccagctc
     2221 aaacggctgg cctcaatggt attcttgcgg gaaaaccagt tgaaaatggt tacctgcgat
     2281 tgcaattgca tgccaagatt aaaaaaatta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatctt ctgatatccc
     2521 atttttcctg gccggatgac atgataaata tcaccagtgc agtggccagc ataaggctcc
     2581 ttttcatagg ctgcaaaaat agtatgatta tagggccctc tgtatgattt tttacagatg
     2641 gttttttaaa tactgttgag tgcctgttcc aggtcagcaa taaggtactg cggatcttct
     2701 aatcctacat acatacggat ataccggtgc tctctgttgg aagtgtcaaa ttcggccatc
     2761 ggaatgccag cacatttggg tattaccagg ctttcatgcc cgccccagct aacagccatc
     2821 ataatatgtt tcagtgactc acaaaattgt acaatactat ctcttttacc tgttttaagt
     2881 gtaaaagtga ataggccgca ggcccctttc atttgatctt tggcgagtgc aaactgggga
     2941 aagctttcat caagcgggaa aatgagggat tccaccttcg ggtgctgttt catgaatgaa
     3001 acaactgtcg cggtagattt tgttattcgt tcaattcttg ccggtagggt tctaagacca
     3061 cggatcatca gccaggcgtt gaaaggttgt ataccgctgc cgatattcag gtattcactg
     3121 tcgaatattt tttttatcat ggtatgtgaa ccacaaagta ctcctcccaa tgtatcactg
     3181 tgacccccga tatatttggt ggctgtttgc atcaccatat caatccccat ttcaatgggt
     3241 ttttgataaa gcggagtaca atagctattg tcaattagtg taacaatgcc ttttgacctt
     3301 gctaattctg ccacttcacg gattggttga atggcaaaat cccagctgtt aggagattcc
     3361 aaataataaa agcttgtatt ttctttggtt gcatttttcc agttagcgat agaagttccg
     3421 tcaatataag taacgcttac attgaaccgc ggtaatatca catcaaacat tcgctgcacc
     3481 caggtataag gcgcttttac cgaaacaata tgatcgcctg atttgatatt ggcaaaaata
     3541 ccggcgaaga tggccgcagc cccgttgtta aacaccaggc aatcttccgc cccatccaaa
     3601 gccgccagtt ttttgcgcaa tatatcaaca gtcggattta gaccgcggct atacaggtac
     3661 cctcccattt catcttcaaa tgcctttgaa agagctgcta cattcttgaa agcaaaattg
     3721 ctggtctgta taatgggcgg ggcaatagcg ttgaaataat ggtcccggtc ttcccccagt
     3781 tcattgatga tttgtgaaat atccatatca ggaattttgt tgatggccgg gtcgtaaggc
     3841 cttcacggca aaataaataa gaataaagcc ggctagtaca cccagcccaa ttagtgataa
     3901 tttggtttca ttaataagca ggctgatggc tacaaatgaa taggctgcta taaagacaag
     3961 gggcagcaat ggataaaatc tcatactgta tataccggtg ccattcaaat tagctgtcct
     4021 ttttcggata ataaaaatac tgcctgcaga aagcaccatc ccgaaacaat caagaaaaat
     4081 agtaaagctt aaaagggtat caaattcttt ggcccagaaa acaatcaggg ctgcaatcac
     4141 ggcaaataag gtcagggcca gcgtaagggc gccccggctg tttcgttgct gaaaagcagc
     4201 cggcagtatt ttatcttcac tcatggccac catcacccgg ggattactta agagtaacac
     4261 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacaaaaccc
     4321 tctctgcctg cactccaaaa attttggaag ccatgatcgc tgcaatgttc ttgctggttt
     4381 tcagctcttc aaaaccaata acctctacat aagcatagtt aatggtaagg taaagggtaa
     4441 taatgataaa aatacccatg aaaattcccc ttggaatatt ccggttgggc ttttccactt
     4501 ccgccccaaa gttgatggtc tgctggtagc ctccgtaagt aaaactaaca gccaccaagc
     4561 ctacaccaaa agctttgata tattctttaa aaccgggatc aatggaagga gaaatcaggg
     4621 aagacacacc attatcacta cctgatgcaa aaaataatgg ggttatcagt agtagtacca
     4681 gcccaatttt aatgatggta agcaccgtct gtgttttggc acttaaacga agcccaagca
     4741 tattcacacc ataaaaaatt gctatcgcag caagggcgat ggttatatgg atagtttgaa
     4801 tatacgcttt gtcggttgca tgtggtaata taactccggt gatatactca cttcctacca
     4861 aagctacagc ggcccccgaa gccgcattgc ttaccagtat aatacagttg atagcaaagg
     4921 caatggaagg atgataagca taggaaaaaa ctttgtaata acctcccatt accggcaaac
     4981 gggatccgat ctcagcataa gttagggcac cgcacaaagc cacaatgcca cccgcagccc
     5041 aggcaataaa aaaaataagc ggagtaggtg atgcagccgc tacattggcg ggggttctga
     5101 aaatacccat accaatcaca aggctgacaa cgatcatggt taaatcaaag agtcctaact
     5161 tgttcctgga tgtcatggct tgaagataca aataaaaggt tctaacttta accagctaaa
     5221 atcctgtttc tgtgaagaag accatcctgt ttgttgtgct ttcacttcac ctgttgttca
     5281 gccatgctca gactgaaaaa gcagacactt caaaaacatt ggacagtatc attgttcaat
     5341 cttttgaatt gaataaacct gctacattct ctgctatacc cgtcagcagg ttattggtta
     5401 gcatcgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aacgggttga atgcagtagc
     5461 tggtgttcga atggaagaaa gatctccggg tagttaccga attaatatca ggggtagttc
     5521 tcttcgatct ccttttggtg tcagaaatat aaaagtgtac tggaatggta ttcctgtaac
     5581 cgatcccggt ggcaatacat acttcaacca gttcgcctgg aataattttt catccattga
     5641 aattactaaa gggccatccg gcagtatgta tggagccggt actggcgggt tgatactcct
     5701 gaataattgg caaccgggtg caaacctgga atacaatacc ggcagctata acctgcacaa
     5761 tatttttact tcggcaaaat ttggaaacaa agacaacaga agctacatca gctatgcaca
     5821 tacacaaaca gatggttacc gggtacaaac aaatatgcgc agagataatt tttcctggca
     5881 aaccaaatgg agcaaagaaa aatatgaact gacagcttct gtattgttta ctgatatgta
     5941 ttaccagacc cccggcgcat taaccttgac agaattcagt aataatccga aagccgcccg
     6001 ccctgccggc ggaggtttcc ccagtgctgt aaatgcaaaa gcagccatct ttcaaaagaa
     6061 tttcctggca ggcattaaca actattttat catttctcct tccataaaga ataacacaat
     6121 tatttacgga tcttacgcac aggtaaaaaa ttcagctatc aggaattatg aaagaagaac
     6181 agaaccgtca ttcggcggtc gttctgtttt cagctggaga aaaagcaatc ctcaatctga
     6241 ttggaatata atagcagggg cagaattcca gcaaggttac tttaacacac aggtatcaaa
     6301 gaataaaaac ggcaacccgg ataccttaca aacaaacgat gatattgtta caagcaacct
     6361 gagcattttc acacaatcca gtattgacat tggtcaaagt tggtcagtca tcgggggaat
     6421 tagtattact aaaacaaaac tggagctgac aaggcttagc agttacccgg ttatagcaca
     6481 atcccgaacc taccgaaatg agctggcccc aagactatca atgatgaaaa aaataaatga
     6541 aaacatacac tggctggctg cagtatcaaa aggtttctcc cctccaactg ttgcagaagt
     6601 attgccctct accggggtca tcagtactta cctcgaagca gaaagaggct ggaactacga
     6661 aacaaacttt cgtttttttc tgctgaaaaa ccggttaaag ctggaactta ccgggttcta
     6721 ttttaagctt aacgaagcat tggtacagcg aagagatgct gggggagccg atttttttat
     6781 caatgccgga gatactaaac aaaaaggcat tgagttccat gccgattatt caaaaaactg
     6841 ttttggcaac atcatcagca aattcaatat ccgaacagat tttactttca atcattttcg
     6901 gtatgggtat tttgtaaaag gcactgacga cttctccggg aataaaatac cttcagttcc
     6961 ttccactgtg tttactgtac tggcggatct gtattcttac cagggcattt atgtaaatgc
     7021 aaattactac accgcttctt ccatttacct gaatgacgcc aatactgcca aagccgagcc
     7081 ttaccacttg ataggttgcc agctgggctg gaagaaaaga gaaaaaggac cttnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgaaacata
     7201 tagcctgggc aatgatatca atgcagcagg tggccgctat tacaatgccg ccccaaaaag
     7261 aaatttctat atcggcattg ccttgcaatg gttgaaaaaa taaaagtcac tatcattagt
     7321 acttaacaac agggcacccc agtatatctt tttgaccaat acggtaactg accccggcag
     7381 aaatggtatg ataatgatcg ttcttctccg ggttggctgc tttgctgaat ccatccaggt
     7441 aatcggtaaa caacaaacgg taagatgatt ccacattaac accaatacgc tgggtaagcc
     7501 ggtaacgaaa ccctgcccca acaggtataa cgggtatgga acggggtggc ctttctgcct
     7561 ggtcctctcc cagtctttca attagctctg gttcttcgcc aaaatgtgat gcattgaact
     7621 ggctccagtc tcgtttgata cgcaaaaaac taatcccccc gcctgtaaac aaatagggtg
     7681 aaattctgcg gctatcatac tgatcagcta aagggctcca ggatagcaat acactaagct
     7741 ctgttaccgg tgttctgaaa ttaaaattcc gttgttgccg gtaaacagga tatttatata
     7801 aagcatcatc gcctcttaag ccaccataag aaaaattacc ccgcagggca aaggatgggc
     7861 tcagcaataa actgccgaac aacgaaaatc ccaaccgggt ggtttcaaaa gaaccaaagc
     7921 gttggggggt caggtcgccc tggtaaacaa atccgcttat taaagcgcca atttcatacc
     7981 ggggacggtt aagttgtgca gcgaccggct cacttgacaa aaaagaggat acacaaataa
     8041 gcatcacttt catcaggctg ttgttcatag ttcacatgtt taaagaaagc aaaggctttc
     8101 cggggtaatg aataacagca tctttggtag tagaatttcc ggacaggttt acaaaaactg
     8161 gaatggtcaa cgcattatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnntttt atttttattt cgacatacaa accccttcag cggttgaaat
     8341 ccccgtcagt ggtcaaagac cctgacggcg gtttaataat taataacctt tgttaataac
     8401 tctttcaaca aaaatcaatc atccccctac ttttgcaccg gtttggtttc ccggtacata
     8461 ttatcgggat taaaagggaa atccggtgta cttgcctgcc gaaggttcgg agtccggtgc
     8521 tatccccgta gctgtaaagc ctgtgaatgg gtaaaaggtc aacaaaaaag aattcattat
     8581 ttattattga ccaccctcca ctcacctgct gaatttgtaa accactgttg taaaaacaac
     8641 gggaaggtgt tcagcactaa gctaagccag aagacctgcc agatcaacgt aaacattttt
     8701 cagctttcgg gtgaaaggct tgaagattta agagtgtaag ggcattcttt tattttctac
     8761 ttttttattt cctggaagca gtcacacaaa atttcattaa tcaatgaaaa gaattttttt
     8821 tgtgctggct gctgtcatta tcagcacaca gctaaaagcg caacaagaca ccacacagtt
     8881 ggaagaagcc gttatcacgg ccagcaagta cgaaagaaaa caggcggaaa cgggtaaagt
     8941 aatgaccgtc atcagcaggc aggaattaga aagaaacagt ggccgaacat tagctgagtt
     9001 attaaacacc acaactggtg tcaatatcgt tggagccaat aataatgcag gaaccaatct
     9061 gactaccagt atccgcgggt ctgcagccgg caatgtattg atattgattg acggtattcc
     9121 tgttaatgac ccgtcggtaa taacaaacta ttttgatctc aactttatgg tcaccgacca
     9181 ggcagaacga atagaaattt tgaaaggagg tcagtctacg ctttacggat cggatgcagt
     9241 ggcaggtgta ataaatataa tctcccggca gcagcggcaa aacggagtaa aaataaatgg
     9301 cagttttacg ggaggaagtt ttaatacact caaacaacat atcggcctga acggacgtaa
     9361 caacaaaacc agctacggta tcaactacac acacaccagt gctgacggat tctcatctgc
     9421 ctatgattca tcaggatctg gtacgtttga caaagatggt ttaaaccagc ataccctggc
     9481 agccaggttg ggatttccgc tgtcaaaaag tattacggca gatttttcag gcacctataa
     9541 tcattataaa acagacttgg atgcagcagc ttttactgat gaaagagatt ttactgctgt
     9601 aaaccagaat aaacaatttg ccgtaggtct tacccataaa catagtaagg gagcagtaag
     9661 attaaactat cattacaact ggctgcgcag gtattacctg gatgactctt cacataaaag
     9721 cagcccatac atagattatg taaaaagtaa ttatatcggc agaacgcagt actgggaagt
     9781 attttccaac tggcgactga ctaattggga gatattggcc ggaatggact accgcctaaa
     9841 cacaaccgat caatggtatt ggtcaacggg tgcatttgga ccttacgccc cacccaaatt
     9901 gaatgcggaa atgaaacaac tcagccccta tgcatcggta gtatataaat acaaaaaact
     9961 aactgtggaa ttgggaggtc gttttaataa acattcagaa tatggtaata atttcacttt
    10021 cacattcaat ccctcgtgga gattgaattc aggcataaaa gcttttgcca acctgtattc
    10081 tgcattcaaa acacccacac tctaccagct cttcgatgat tttgcaggaa atcccggcct
    10141 tactcccgag aaaggcataa ttgctgaagc aggagcagaa atgctaaaag gaaaaagttt
    10201 tacagcaaga gtggtcggct tttacaggaa aaccaatgat gttattatct attcatttaa
    10261 tccctccgat tttaccagca ggtacatcaa tgccagcaag caaatcaatt acggcgctga
    10321 actggaagcc ggttacaaca aaggaccctg gaagatcagt gccaattata cattcaccag
    10381 cggaaaaacg actgccgcct ttgatggaac aggcacagct ttgggtaaag acaccagtta
    10441 ctttaacctg tatcgtattc caaaacatgc atttaacctg gatgccggat tccgggttaa
    10501 tcaatcattg tatgttagta cacagttacg cagnnnnnnn nnnnnnaatt catctacggc
    10561 gctccgcccg ccactctcga tgcctataac accattgata tgtatggtga atacaaattc
    10621 agtagcctga tcagaacatt cattcaattg aagaatatta caaacaagca atattttgat
    10681 attctcggct ataatgcaag aagatttaat atcacggcgg gcattaattt tcagctctga
    10741 atcagttcaa tatgtctgca caagaattaa aaagctgccc ccgctgcaat aaaacatttg
    10801 aatgcaatcc gggacatata gcacaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tttgtagtaa ttgcctcaag cagttagccc
    10921 tggaattaga tctctttaaa gagaaatata tcttcagata actacatttg cacaaaacca
    10981 gcagttatgc aatttcacct gcaggagttt ctgaccctca cctttacact atttgctgtt
    11041 attgatattg ttggctctgt gccgatgctg atttctatca agcaaaaaat gggagatatt
    11101 catccgctaa aggtcacact gatttctgga gcattgatgg tgcttttttt ctttattggc
    11161 aaaccattcc tgaatgtgct gggagttgat atcaagtctt tcgcagtggc aggttctttt
    11221 gtaatattta tcctcgggct tgaaatgatc cttggtattg aattcttcaa ggccgaaaaa
    11281 ggaacacctt ccgggacagt agtacccatt gcctttccgc tgattgccgg aagcggaacc
    11341 cttactacca tcatgtcatt aaaagccaca tttgaacgaa gtgataagaa tgaatacatg
    11401 atactgctgg ccatcctggt taaccttatt gttatttacc ttgtaatccg ctctctaaat
    11461 cttattgaaa aagtattggg aaaagccgga ttaatggcgg tacgaaaatt ttttggcgta
    11521 attttgctgg ctattgccgt caaagtcttt gcagctaatg ccccgggatt gataaagtag
    11581 tttgagtaag gaagagttaa gtttgatatc agcaacttca aacactaaac ctcaaacttc
    11641 aaacattacc aggcctcgta gtacaatgga tagtataaga gtttccgaag ctccagatac
    11701 aggttcgatt cctgtcgagg ctactagtgt tccaaaataa attttattta cagaaatccc
    11761 cgcccgaagg caccatttgg gcggggattc ctgtaagtgc tgctaaaacc attctctgcc
    11821 ggtgaatgct tttttctttc tatagaaaat ttgatacctt ccttattgaa tcaaatcatt
    11881 aattatgcca acgcaatca
//
LOCUS       DAORLS010000063        11726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2929_length_11726_cov_1.935053, whole genome shotgun sequence.
ACCESSION   DAORLS010000063 DAORLS010000000
VERSION     DAORLS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2929_length_11726_cov_1.935053"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1073
                     /locus_tag="PK977_06660"
     CDS             162..1073
                     /locus_tag="PK977_06660"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRF17828.1"
                     /translation="MRISFLLAAISTSFVLNAQPPQVPLYSIDNVTLPESLNKQVCIS
                     GMKYLDGKLYMASERCRVIFAFDPETKTVINSINLNVPQFFEMEGMTSYNGKLYLVSE
                     NTVAVYEVDATSSSIRIVETSTPLPPKSKSGDGMEGIAANETHNKFYILRERNEDMSK
                     SQIYTYSVERNFEDVLSLKYESVIELPLLNPQWRYSDICFDSQNNRLICLKSFSKGKL
                     RQQFIETINIDVNGNLQAETLQNLPVENFSEISNQYKDQDYSMNLEGITLDKDGNIWI
                     ISDNTSGKAQCDMVAKEKTILLQLKRK"
     gene            1079..>1418
                     /locus_tag="PK977_06665"
     CDS             1079..>1418
                     /locus_tag="PK977_06665"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HRF17829.1"
                     /translation="MAEMKETALRSALLQKILLAVIAGIGWYALSLQFYLMVQNSEAN
                     NMTVTRSVTNFFSYFTILSNLLVAVSTTARLLSPTSSIGRFFSGIRTYSAICLYITIV
                     GIIYSIALRHI"
     assembly_gap    1419..1556
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1557..1746
                     /locus_tag="PK977_06670"
     CDS             <1557..1746
                     /locus_tag="PK977_06670"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HRF17830.1"
                     /translation="PAAYLIYTLVRGSITGWYPYPFIHAGELGYGKVAINTVMVLVAF
                     IAVGLALIAINRIGKTRS"
     assembly_gap    1941..1978
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1979..2252
                     /locus_tag="PK977_06675"
     CDS             <1979..2252
                     /locus_tag="PK977_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17831.1"
                     /translation="VDGKLSNGRWQPLTSKEIQFSCPNATFSGNELIIPEDFNEEKVT
                     VKAVLKSNPSIFIEKTIWIKKIPDGPLPTKDEILKGIPERKIKRKN"
     assembly_gap    2336..2360
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2450..2812
                     /locus_tag="PK977_06680"
     CDS             2450..2812
                     /locus_tag="PK977_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF17832.1"
                     /translation="MNSNDLQKRLKEFAYRVVPLCESLPGRKVSRVIEDQLTRSAFSA
                     AANYRAACKAQSTKAFAAKLSIAFEEIDESLFWLEVILDLNLLKVEKLSLIIKEADEL
                     TRILASSRKTSQNKLKRP"
     gene            2851..3504
                     /gene="fsa"
                     /locus_tag="PK977_06685"
     CDS             2851..3504
                     /gene="fsa"
                     /locus_tag="PK977_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRF17833.1"
                     /translation="MKFFIDTANLAQIKEANELGILDGVTTNPSLMAKEGIKGEEAVM
                     NHYRTICEMVDGDISAEVISTEFKAIIEEGKKLASIHPNIVVKVPMIKDGIKAIKWFS
                     DNGIRTNCTLVFSAGQAILAAKAGATYLSPFIGRLDDSNWDGIELISQIRHIYSIQGF
                     KTEILAASIRNALHIVKCAEAGADVCTCPLDSILGLLKHPLTDIGLAKFLEDAKKTL"
     gene            complement(3578..4858)
                     /locus_tag="PK977_06690"
     CDS             complement(3578..4858)
                     /locus_tag="PK977_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRF17834.1"
                     /translation="MIRNSLLLLLLLSFSATAQLPDHVYKSNIKTVKLFKSGDMYSYP
                     VIMLNSNEQLELHFDDMDADVKYYYYSFQLCNADWKPANIQLFDYIRGFTSNRITNYR
                     HSSLAQTRYTHYQAVLPERNGAPSKAGNYLLKVFLNDDTSKLVFTKRMLIVNTKANVS
                     AQVLQPFNTNYFQTHQRVQVNVSTVPGQVNTFSPQDLKVVVLQNNIWNNASLLDRPTV
                     FRGNYYEYSDEENTTFQSGKEWRWIDLRSLRLMSDRMTKIVDNDTTNRVDVYVKPDGE
                     RRQQLYIYYRDFNGIYTVENRDNSNALWQSDYAWVHFTYVPPGNRAYEGKSVYLFGEL
                     TNYLQDDNSKMIFNEEKGVYERALYLKQGYYNYSYVTLTDKKQPGVQALYENTEGNYW
                     GTENSYMVLVYFRNFGARADELIGFTKINSAFQR"
     assembly_gap    4996..5036
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5037..6254
                     /locus_tag="PK977_06695"
     CDS             <5037..6254
                     /locus_tag="PK977_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF17835.1"
                     /translation="GGRKSFSRFIIRLSTVATVISVAVMIITLSLANGFQETVSQKVF
                     SFLGHLRVQEKQPGKAIISEEIPIIQNDTLVREIKQNPLVKNIHPFATKYAILKTKEE
                     IEGVLVKGFDSTYDFNHIKGFIKQGRPIQFNDSTYSREIMISDFTAKQLQLKVNDRIL
                     IYFIRPGSSAEEGLPNIRPDKLTITGIFKTGIEDYDRTFAIGDIKLIQRLNDWQPDEA
                     GGYEIFLHDYKMTDEAVKEIYEIADFPITWDTVSARDISPNIFDWLNMQDVTRNVLIA
                     FMVIVAVINLITCLIILVLERLRMIGILKALGATDWTVQDIFLRHSLIIAIRGILLGT
                     LIALGLLWLQDATGFIKLKEEAYYLSTAAVKIVWWQVAAIGIGTLLICLLVLMIPTFL
                     VKRIQPVKAIRFN"
     gene            complement(6256..6993)
                     /locus_tag="PK977_06700"
     CDS             complement(6256..6993)
                     /locus_tag="PK977_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRF17836.1"
                     /translation="MLVKSKVKYIQSLGQKKFRQQEGVFVAEGPKLVKELLTEHSDSV
                     VEVFALKNWIDENKRVTINCNLSEITEAELEKISQLTTPNQVVAVVKHFNTIKPFAEK
                     GKFILALDGIQDPGNMGTIIRIADWFGVQHIVCSQDSADWYNPKAVQSTMGSIGRVNV
                     FYTSLGKWLEEQNDALIYAAVLGGEDLTKMQQPAGGVIVIGNESKGISEEVLKFCNAS
                     ITIPQKGKAESLNAAVATGIILSHFCK"
     gene            7014..9335
                     /locus_tag="PK977_06705"
     CDS             7014..9335
                     /locus_tag="PK977_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF17837.1"
                     /translation="MSARHYYFSRNYLYTLAAGCFIFFISSCTIVKNYPQGKPFVYKT
                     NINVIGNFTNEEKETLASRLKGQLDDSMRSRSVSKVAWSVMKNPPVFETENADKSMLF
                     MKALLKSLGYFSDSISYQSQIDTVNKQEYRTTINFDVKPGKVVRIDSFAYNFKNTELQ
                     TLAIANQKNALVKKGDPLAKSAISIELDRLVDMYRNNGYMRFGREEMIGLWDTLDVSL
                     LKPTFDPLEQAAILQKLRERRENPTANLEIRLKPGYDSNKLKKYYIGNVTVYPDLVYD
                     TTGFTTKETTVDGVKIISYRNMFRPRTITRNIYFKHGDLYNLKKYVKTINRFNSLGAW
                     RLVNIEQEVRKGQDTADFIVRLTPAPKYSFTTNIEGSQNFNAVTGNLFGFGINLSLLN
                     RNFAKSASQGTTSIRFGVELGNQFVQTQQASVSHNIYFPKSIPQFKWFSEKLTNNIRT
                     LFSFNAANTERRLLYNLTTVNGAWGYEYQSTNAERNQLFQLVWKIPNIEYAYLKPRDS
                     LVKLIANNPVLKNIFTDGFIASTIFSATLKKNLEKRPSVISFNLELPFLAGLIPSKFL
                     DTQLYRFIKTNIDVTKQFKFRNSSFVFHAFAGIGYALNSTVNPDKKNALPFFRQYFAG
                     GPNSMRAWRLRRLGPGSTVKDFGQFPDRFGDVQLEFNTEYRFRLMTVAGTKVESALFV
                     DAGNVWFLKKDAGSDEEVFNFSRLGKDIAVGAGTGLRVDFSFFLIRVDYAWKIKDPSP
                     DPSNEDSQNKWFYGLKQPLKGQLQIGINYPFSL"
     gene            complement(9332..10018)
                     /gene="truB"
                     /locus_tag="PK977_06710"
     CDS             complement(9332..10018)
                     /gene="truB"
                     /locus_tag="PK977_06710"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRF17838.1"
                     /translation="MKEISNSFEEGKVLLINKPLQWTSFDAVRKIRNLIRIKKVGHAG
                     TLDPLATGLLIICTGRFTKKINEYMAQEKEYTGTFTLGATTPTYDLESEPENFKSTDR
                     INVELLKSTTEKFMGEIMQVPPAHSAIKVDGKRVYELARQGKEVKLEPRKITIKEFEI
                     TKVEMPMVHFKVVCSTGTYIRSLANDFGAALGCGAYLSSLCRTRIGSFLLDDAMSIEG
                     FEASIKHIGP"
     gene            complement(10092..>11210)
                     /locus_tag="PK977_06715"
     CDS             complement(10092..>11210)
                     /locus_tag="PK977_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRF17839.1"
                     /translation="FSIGDENKVVLTLHNNYGFPVRTSLIDELPFQFQERKWMRKAKL
                     ERDKPHQLEYHLRPTTRGEYAFGNINLFVHAPLQLVKRHYIIPAEHVVKVYPSYIQMR
                     RYQLLAVSNRLQEAGVKRIRKIGHSMEFEQIKEYVRGDDYRTINWKATARKDGLMVNN
                     YTDERSQQIYCLINKGRVMKMPCHGMTLLDYAINAALVLSNVALVKQDKAGLITFEKN
                     IDSFLLADKKPTQMNQVLENLYRQKTDFLEPDFEKLFSIIRNRVTNRSLMVLFTNFES
                     LESLQREMTALKKIARYHLLLVVFFENTELKSLLDSKTKTLEEIYIKTIAEKFAYEKR
                     QMVKELHKNGIPSILTTPENLTVNTINKYLELKTRMSI"
     assembly_gap    11211..11229
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11230..11428)
                     /locus_tag="PK977_06720"
     CDS             complement(<11230..11428)
                     /locus_tag="PK977_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRF17840.1"
                     /translation="MFKSFHLNNIVYYIGGIAAVVFVLSYFQPALYRIGGLLLLLLLV
                     AILIDMLLVYSKKNGIMAERIA"
     gene            complement(11502..>11726)
                     /locus_tag="PK977_06725"
     CDS             complement(11502..>11726)
                     /locus_tag="PK977_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF17841.1"
                     /translation="IYLGASPRASLAIMNASKATAAMMGRDFVTPEDILSVVTPVLRH
                     RIILAPDKEMEGITEDDVIRQIIQGMDVPR"
BASE COUNT         3375 a         2562 c         2228 g         3300 t
ORIGIN      
        1 atgtcaggtg gcggttctgc aaagcgcaac tatccttctg caaaacacat ctctgctcat
       61 acaattgccg ggtttaggat ggcgaagccc gtcgccattt cccccttttg ccctatatta
      121 gcaaactgcc ccgaggggcc tttttattaa aagtcatcat catgcggatt tcgttcctgt
      181 tagcagctat cagtacctct tttgtgttga atgcccagcc cccacaggtt cccctgtaca
      241 gtatcgataa tgtaaccttg cccgaaagcc tgaacaaaca ggtttgtatc tccggtatga
      301 aatacctgga cggcaaactg tacatggcct ccgaacgctg ccgcgtcatt tttgctttcg
      361 accccgaaac caaaaccgtc atcaacagca tcaacctgaa tgtgccgcag ttcttcgaaa
      421 tggaaggcat gacttcctac aacggcaagc tctacctcgt ttctgaaaat actgtagcag
      481 tttacgaagt ggacgcaacc agctcttcca tccgcattgt tgaaacgtcc actcccttgc
      541 ctcccaaatc aaaaagcgga gacggcatgg aaggcattgc agccaacgaa acgcataaca
      601 aattttacat cctgcgggaa cgcaatgaag acatgagcaa atcgcagatc tatacctaca
      661 gtgtagaaag aaattttgaa gatgtgctca gcctgaaata tgaatcagta attgaactgc
      721 ccctgctcaa cccgcagtgg cgttattctg atatttgttt cgacagccag aacaaccggc
      781 tcatctgcct gaaatcattc tccaaaggaa aattgcgcca gcaattcata gaaacaatca
      841 atatcgatgt aaatggtaac ctgcaggcag aaaccttgca aaacttaccg gtagaaaatt
      901 tctctgaaat atccaaccag tacaaagacc aggattacag tatgaacctc gaaggcatta
      961 ccctggataa agacggcaat atctggatca tcagtgataa tacttccggt aaagcccagt
     1021 gcgatatggt tgccaaagaa aaaaccatcc tgctgcaact gaaaagaaaa taaccggtat
     1081 ggcagaaatg aaagaaactg ctctccgttc tgctttacta caaaaaattc tattggccgt
     1141 tatagcaggt ataggctggt atgcactcag cctgcagttt tacctgatgg tacaaaacag
     1201 cgaagccaat aatatgacgg tgacaaggtc tgtcactaat tttttcagtt acttcaccat
     1261 tctcagcaac ttactggtgg ctgtcagcac taccgccagg ttgctttcgc caaccagcag
     1321 catcggacgt ttcttttccg gcatccgcac ttactcggcc atttgtttat acatcaccat
     1381 cgtaggtatc atttacagca ttgccctccg ccacatctnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccg
     1561 gccgcctacc ttatttatac cctggtaagg ggcagtatta ccggctggta tccttatccc
     1621 ttcattcatg ccggtgaact gggttatgga aaagtggcga tcaatacagt tatggtgctt
     1681 gtcgctttca ttgcagttgg cctggcgctg atagccatta accgaatagg caaaacccgg
     1741 tcttgaatac agaaaaacaa tcagcagcaa cgtatactgt taagctttag ccaacagcaa
     1801 ccctttggca tgaaattgcg acttttgtac aggtatgaag aaatttttac tcctgttgat
     1861 aattaccagt tcgctgtcgc ctctgtttgc acagaaaatt gacagtattt atttccatct
     1921 ttataccgac agcctgaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     1981 tggacggcaa actttcgaat ggccgctggc agccactcac cagcaaggaa atacagttct
     2041 cctgtcccaa cgccacattc tccggcaatg aactgatcat tcctgaagat ttcaatgaag
     2101 aaaaagttac tgtaaaagcc gtgctgaaaa gcaacccttc catttttatt gaaaaaacaa
     2161 tctggatcaa gaaaattcct gacgggccat tacccaccaa agacgaaata ttaaaaggaa
     2221 ttccggaaag aaaaatcaaa agaaagaact aaggcttcgg ccattaatct ttacaccctt
     2281 atctttgcgc cggcaggtct tacacgacca gctcctgctg aaccctccca gggccnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn gcgatgtaat cagcctgcca ttttttatta tttaagattg
     2401 aagaagtaat atttacttat ttaaggttca atcttaaatg tattcttcaa tgaattccaa
     2461 cgacctgcaa aaaaggctta aagaattcgc ttaccgggtg gttcctctct gcgaatcatt
     2521 acctggcaga aaagtttcaa gagtaattga ggatcagcta acaaggtctg cattttcggc
     2581 tgcagctaat tacagggctg cttgtaaggc tcagtcaaca aaagcatttg ctgccaaatt
     2641 atctatcgct tttgaagaaa tagacgaatc cttgttctgg ctggaagtaa tcttagatct
     2701 taatctgcta aaagttgaaa aattgtcact tattatcaag gaagctgatg aattaacccg
     2761 tattcttgcc tcctcaagaa aaacatcaca aaacaaatta aaacgaccct gataaaatat
     2821 taaatactca atcttcaatc ttacatcatt atgaaatttt ttattgatac ggcaaatctt
     2881 gcccagatca aagaagccaa cgagttaggt atcctggatg gtgttactac caatccttca
     2941 ctgatggcca aagaaggcat caaaggtgaa gaagctgtga tgaaccacta caggaccatt
     3001 tgtgaaatgg tggacggcga tatcagcgcc gaagtgatca gtacagaatt caaagccata
     3061 atagaagaag gaaaaaaact ggcatcaatc cacccgaata ttgtggtgaa ggtgccgatg
     3121 atcaaagacg gtattaaagc cattaaatgg ttttctgaca atggcattcg caccaattgc
     3181 acattagtgt tttctgcagg tcaggcaatt cttgccgcca aagctggtgc tacttatctg
     3241 tcacccttta tcggtcgcct tgacgacagc aactgggatg gtattgaact gatctcgcag
     3301 atcaggcata tttattctat acagggtttc aaaacagaaa tactggccgc ttccatccgt
     3361 aatgctttac acattgtaaa atgtgccgaa gcaggcgctg atgtatgtac ttgtccgctg
     3421 gattctatcc tcggcctgtt aaaacatcca ttgactgata ttggccttgc caaattcctt
     3481 gaagacgcta agaaaacctt ataaataaaa agttgattat tgaaaaaacc tgcacccctt
     3541 cagggatgca ggtttttttt taatacaaca agaagtatta tcgctgaaaa gcagaattga
     3601 ttttggtaaa accgatcaat tcatcggctc ttgcaccaaa attgcggaaa tacaccaata
     3661 ccatatagct gttctctgtg ccccagtaat ttccttcggt gttttcatac agcgcctgta
     3721 caccgggctg ttttttatcg gtcagtgtta cataagaata gttgtaataa ccttgtttga
     3781 gatacaaagc tctttcatac acaccctttt cctcgttgaa aatcatcttt gaattatcat
     3841 cctgcaaata atttgtcagt tccccaaaca ggtatactga tttaccttca taggccctgt
     3901 tgcccggcgg tacataagta aagtgtaccc aggcataatc actttgccag agggcattgg
     3961 agttatccct gttctcaacg gtataaatac cattaaagtc gcggtaatag atatacaact
     4021 gctgccttct ttcgccatcc ggttttacat acacatccac ccggttggtc gtatcattgt
     4081 caacgatctt tgtcatccgg tcgctcatca gtcgcaggct gcgaaggtca atccagcgcc
     4141 attccttgcc tgattgaaaa gtagtattct cttcgtcgct gtattcgtag taattacccc
     4201 gaaaaacggt aggcctgtcc agcagtgaag cattattcca gatattattc tgcagtacca
     4261 ctactttgag gtcctgcggc gaaaaagtgt tcacctgccc gggaacagta ctgacattca
     4321 cctgcactct ttgatgagtt tgaaaataat ttgtattgaa aggttgtaat acctgtgctg
     4381 aaacattagc ctttgtattg acgatgagca tacgtttggt aaagaccagt ttgctggtat
     4441 catcattcag aaaaaccttt aataaataat ttccggcctt actcggcgct ccgtttctct
     4501 ccggtaatac agcctggtag tgggtatacc ttgtctgcgc cagcgaagaa tgacggtagt
     4561 tagtgatacg gttactcgta aacccacgga tataatcaaa cagctggata ttggcaggtt
     4621 tccagtcggc attgcacaac tggaaactgt aataataata ttttacatct gcatccatat
     4681 catcaaaatg cagttcgagt tgttcattgg agttaagcat gatgaccggg taactgtaca
     4741 tatctcccga cttgaacagt ttgaccgttt taatattgga tttataaaca tgatcaggca
     4801 gctgtgctgt tgcactgaag gatagtaata acaaaaggag aaggctattt cgtatcatat
     4861 aaataataaa agtcagaatg aactacaata ttgcttaaaa taagctaaaa aagggaagaa
     4921 atcttatcaa ccaccttatt tttgaagaag acaaacaatt caacttgaag gttgcaggat
     4981 ttatagctaa ccgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcg
     5041 gcagaaaatc attctcccga tttattatcc gtttatcgac tgtggctaca gtgatcagtg
     5101 tagcagttat gatcattacc ctctcactgg ccaacggatt ccaggaaaca gttagtcaaa
     5161 aagtatttag ctttttaggg cacctgcggg tacaggaaaa acaacccggt aaagccatta
     5221 tttcagaaga gattcctatt atccaaaatg atacactggt cagggagata aaacaaaatc
     5281 cgctggtaaa aaatattcac ccctttgcca ctaagtacgc tatactgaaa acaaaagaag
     5341 aaattgaagg agtattagta aaaggatttg atagtaccta cgacttcaac cacatcaaag
     5401 gcttcatcaa acaaggaagg cccatacaat tcaacgacag tacttacagc agagagataa
     5461 tgatctccga ctttactgcc aaacaattgc agctgaaagt taacgacagg attctcattt
     5521 atttcatcag gccgggcagc agtgcagaag aaggcttacc gaatattcgt ccggacaaac
     5581 tgactattac cggtattttc aaaaccggta tcgaagatta tgaccgcaca tttgccattg
     5641 gcgatataaa actaattcaa cgactgaacg actggcaacc ggatgaagcc ggcggttacg
     5701 aaattttcct gcatgattac aaaatgacag acgaagcagt aaaagagatt tatgaaattg
     5761 ctgactttcc tattacatgg gatacagtaa gtgcaaggga tatttcaccc aatatttttg
     5821 actggctgaa tatgcaggat gtgacccgta atgtgctgat agcattcatg gtgattgttg
     5881 cggtgatcaa tctgatcacc tgcctgataa ttttagtatt agaacgttta cgtatgatcg
     5941 gcatattaaa agccttaggc gccaccgact ggaccgtaca ggatattttt ctccgtcatt
     6001 cactgattat tgccatcagg ggtatcctgc tcggcacttt gatcgcttta ggtcttttat
     6061 ggctgcagga tgcaacaggg tttatcaaac taaaagaaga agcttattac ctgagtaccg
     6121 ctgccgttaa aattgtgtgg tggcaggtag cagctattgg tattggaaca ttactgatct
     6181 gcctactggt cttaatgatc cccacctttc tcgtcaagag aattcagccg gtgaaagcaa
     6241 tacgcttcaa ctgatctatt tacaaaaatg agaaagaata atgccggtag ctacggctgc
     6301 atttaatgat tctgctttcc ctttttgagg tatagtgata ctagcattac aaaattttaa
     6361 aacttcctca ctaattcctt ttgattcatt gccaataaca atgacacctc cggctggttg
     6421 ctgcatcttt gttaaatcct cgcctcctaa aacggcagca tagatgaggg catcattttg
     6481 ttcctccaac catttaccca gtgatgtata aaaaacattt accctgccaa tactgcccat
     6541 ggtcgactgt actgctttcg gattatacca gtcggcgcta tcctggctgc aaacgatatg
     6601 ctgcacacca aaccaatcag caatgcgaat gatagtcccc atgtttcccg gatcctggat
     6661 accatccagt gccagaatga attttccctt ctctgcaaaa ggttttatag tgttgaaatg
     6721 ttttaccact gcaaccacct ggttaggcgt ggtaagctgg gatatctttt cgagttcggc
     6781 ttcagtaatt tcagaaagat tacaatttat ggtaaccctt ttattttcat cgatccaatt
     6841 ctttaaggca aaaacttcta ctactgaatc tgaatgttct gttaatagtt ctttcaccag
     6901 tttagggcct tcggcaacaa atactccctc ctgctgcctg aattttttct ggcctaaact
     6961 ttgaatatat ttgacctttg atttaacaag catctttctt cgtttactta tttatgtcag
     7021 cccgccacta ttattttagc aggaattact tatataccct tgcagcaggc tgttttattt
     7081 ttttcatttc ttcctgcact atcgtaaaaa attacccgca gggcaagccc tttgtttaca
     7141 aaacaaatat caacgttatt ggcaacttca ccaacgaaga aaaagaaaca ctggcctccc
     7201 gcctgaaagg gcagctggat gacagtatgc gatcccgttc cgtttcaaaa gtagcctgga
     7261 gcgtaatgaa gaacccgcca gtattcgaaa cagaaaatgc tgataaatca atgttgttca
     7321 tgaaggccct tcttaaatca cttggttact tctcagacag tatctcttac cagtcgcaga
     7381 tcgacactgt caataaacag gaatacagaa caactataaa ctttgatgta aaacccggca
     7441 aagtagtcag gatcgactca tttgcttata attttaagaa tacagaactc cagacacttg
     7501 caattgccaa ccaaaaaaat gcattggtaa aaaaaggaga ccccttggcc aaatcggcca
     7561 tttcgataga gttagacaga cttgttgata tgtaccggaa taacggttat atgcggttcg
     7621 gccgggaaga aatgatcggc ctctgggaca ccctggatgt atcattactg aaacccactt
     7681 ttgatccgct ggagcaggca gctattttgc aaaaattaag agaacggaga gaaaatccta
     7741 ctgccaacct tgaaataaga ctgaaaccgg gctacgacag caacaaacta aaaaaatatt
     7801 atatcggaaa tgtcactgta tatccggacc tggtctacga tactaccggt tttactacta
     7861 aagaaacaac agtagacggg gttaagatca tctcctatcg taatatgttc cgcccacgaa
     7921 caatcacccg taatatttat tttaaacatg gtgatctcta caatctgaaa aaatacgtaa
     7981 agacaatcaa tcgttttaac tcactgggtg catggcgact tgtaaatatt gaacaggaag
     8041 taagaaaagg acaggatact gcagatttta ttgtaagact taccccggct ccaaaatatt
     8101 ctttcaccac caatattgaa ggaagccaga atttcaatgc agttacgggc aacctgtttg
     8161 gctttggtat caacctgagc ctgctaaacc gaaactttgc gaaatcagcc agccagggaa
     8221 ctacttccat tcgctttgga gtagaactgg gcaaccagtt tgtacaaaca cagcaggcca
     8281 gtgtgagtca taatatttat tttcccaagt caataccgca gtttaaatgg ttttctgaaa
     8341 aactcaccaa taatatccga acactttttt catttaatgc agccaatact gaacgacgtt
     8401 tattatataa cctgacaact gtgaatggtg cctggggtta tgagtaccag tcgacaaatg
     8461 ccgagagaaa tcaattgttt caattagtct ggaaaatacc caatattgaa tacgcttacc
     8521 tgaaaccaag agacagcctg gtaaagctga ttgccaataa tcccgtactc aagaatattt
     8581 ttacggatgg tttcattgca tcaacaatat tcagtgccac cctgaaaaaa aacctggaaa
     8641 aaagacccag tgttatatcc ttcaaccttg aattgccatt ccttgccgga cttattccaa
     8701 gtaaattcct tgacacgcag ctgtatcgtt ttatcaagac caatattgat gttaccaaac
     8761 aattcaagtt ccgcaattca tctttcgtat ttcatgcttt tgcaggcatt ggttatgcgt
     8821 taaattccac cgtcaacccg gacaagaaaa atgcacttcc cttttttcgt caatattttg
     8881 ccggcgggcc taacagtatg cgggcatgga ggctcagaag attaggaccg ggttcaaccg
     8941 tgaaagactt tggccagttt cccgacaggt ttggagatgt acagctggaa tttaatacag
     9001 agtaccgctt caggctgatg actgttgccg gcaccaaagt agaaagtgct ttgtttgtgg
     9061 atgccggtaa tgtttggttc ttaaaaaaag atgcgggttc tgatgaggaa gttttcaact
     9121 ttagcagact tggaaaagat attgccgtag gtgcaggtac aggtcttcgg gtagacttct
     9181 cttttttcct gataagagtt gattatgcct ggaaaataaa agacccaagt ccggatccgt
     9241 ccaacgaaga cagccagaat aaatggtttt atggattaaa acagcctttg aaaggtcaat
     9301 tacaaatagg tatcaattat ccgtttagcc tctaggggcc tatatgtttt atacttgctt
     9361 caaatccttc tatactcatg gcatcatcga gcaaaaacga accaattctt gtacggcata
     9421 aactgctgag gtaagcgcca caacctaacg ctgcgccaaa atcattcgcc aggctacgga
     9481 tatatgtacc ggtagaacaa acaaccttaa aatgaaccat gggcatttcc actttggtaa
     9541 tttcaaattc ttttatggtg atctttctcg gttcaagctt cacttctttt ccctgccggg
     9601 ccagttcata tactcttttt ccatccactt taatggcaga gtgagcaggt ggcacctgca
     9661 tgatctctcc catgaacttt tcagttgtgc ttttcagcaa ttccacattg atcctatctg
     9721 ttgatttaaa gttttcaggt tcactttcca ggtcgtaagt gggagtagta gcacccaggg
     9781 taaatgtgcc ggtatactct ttttcctgtg ccatatattc attgatcttt ttggtaaacc
     9841 ggccggtgca aatgatcagc aaaccggtcg ccaatgggtc caatgtacct gcatgaccga
     9901 cttttttgat acgaataaga ttacggatct ttctcacagc atcaaaagaa gtccattgca
     9961 gcggtttatt tatcagtaat acttttcctt cttcaaatga attacttatt tccttcatcc
    10021 ggcaaaagta agccttaggc cggctataat tcttacttct tacttcttac ttcttacttc
    10081 gtacttccgg cttaaatgga catcctcgtc tttagttcca ggtatttgtt gatcgtattc
    10141 accgtcaggt tttccggcgt ggtgagtata gaggggattc cgtttttatg cagctctttt
    10201 accatctgcc ttttttcata tgcaaatttc tctgcgatgg tcttgatata aatttcttcc
    10261 agcgtctttg ttttgctgtc gagcagtgat ttcaattctg tattctcaaa aaagactaca
    10321 agtaacaaat gataccttgc aatctttttc aatgccgtca tttcccgctg caggctttcc
    10381 aatgattcaa agttggtaaa caaaaccatc aggctgcggt tggttacccg gttgcggata
    10441 atagaaaaaa gtttctcaaa atcaggttcc aggaaatccg ttttctgacg gtacaggttt
    10501 tccagtacct ggttcatctg ggtaggcttc ttatctgcca ataagaaact gtcgatattt
    10561 ttttcaaaag tgatcaggcc ggctttatcc tgttttacaa gggcaacatt ggataaaacc
    10621 agtgcagcat taatggcata atccagcaaa gtcattccat ggcaaggcat tttcatcacc
    10681 ctgcctttat tgataagaca atatatttgc tggctgcgtt catctgtata gttattcacc
    10741 atcaaaccat cctttcgggc ggtagctttc cagttaatag tacggtaatc atcccctctg
    10801 acatactctt tgatctgttc aaattccatg ctgtgcccga tcttacgtat acgcttcact
    10861 cctgcttcct gcaaacggtt actgacggcc aataactggt aacggcgcat ctggatgtag
    10921 gacgggtaca ctttgaccac atgttctgca ggaattatat aatgacgttt taccagttgt
    10981 aagggagcat ggacaaacag gttgatatta ccaaatgcgt attcaccacg ggtggtgggg
    11041 cggagatggt attccagttg atgaggctta tcccgttcaa gctttgcttt tctcatccat
    11101 ttcctttcct ggaactgaaa tggcaattca tcaataaggc tggttcgcac cggaaagcca
    11161 taattattat gcagggttag taccacttta ttctcatccc caatactgaa nnnnnnnnnn
    11221 nnnnnnnnnc ggctattctt tcagccataa taccattttt tttgctgtag accagcaaca
    11281 tatcaatcag aatagccaca aggagtaata acagcaacaa tccgccaatg cgatagaggg
    11341 caggctgaaa atagcttagc acaaatacca cagcagcaat accgccgatg taataaacta
    11401 tattatttaa atgaaatgat ttgaacactt tgcttttgat atttaaactc cgttccggtc
    11461 atactactaa ctcactacta tcgactaaca attctttact atcatctcgg cacatccata
    11521 ccttgtatga tctgcctgat cacatcatct tctgttattc cttccatttc tttatcaggt
    11581 gccaatatga tgcggtgacg taaaacaggt gttactacag acaatatatc ttccggtgtc
    11641 acaaaatccc ttcccatcat ggcagctgtt gccttggatg cattcatgat ggccagcgaa
    11701 gcccttggtg atgctcccag gtaaat
//
LOCUS       DAORLS010000064        11665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2940_length_11665_cov_2.472438, whole genome shotgun sequence.
ACCESSION   DAORLS010000064 DAORLS010000000
VERSION     DAORLS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2940_length_11665_cov_2.472438"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>408)
                     /locus_tag="PK977_06730"
     CDS             complement(<1..>408)
                     /locus_tag="PK977_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRF17842.1"
                     /translation="AQQAYKVPARDFIDNKSFAADAINELKQDFPELSDYTAIHYLIN
                     HESFVLSGKEQELIESIEVKNNFNPTKTQAEEILQRYSRIRQIMQQAVSEPDPLQKSL
                     FTERLDNIFLHRRWGYLILLAVLFLLFQSVFWLA"
     assembly_gap    409..418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<419..924)
                     /locus_tag="PK977_06735"
     CDS             complement(<419..924)
                     /locus_tag="PK977_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRF17843.1"
                     /translation="MKNNRLHIALVGNPNSGKSSLFNCLTGLNQKVGNFPGVTVDKKT
                     GSSQISPSQTAEIIDLPGSYSLYPKRMDEWVTYKVLLGQDREMRADVIVAVADASNLK
                     RNLLFCTQLIDLKVPVVIALTMMDMAKRKGIKIDIPALERELGVPVVAVNPLKNKGIP
                     ELKKAIALT"
     gene            1106..2764
                     /locus_tag="PK977_06740"
     CDS             1106..2764
                     /locus_tag="PK977_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF17844.1"
                     /translation="MVSSLLRVSVIALLLVACAAPKQASKTDKQLIENLKTHISFLAD
                     DKLEGRRTGSAGEQLAMEYISSQFKTIGLSPKGTSGFYQPFEVNDGKIMKLSSLFMIN
                     GDGLVEERDYFAFPFSPNTSIDAFVSPSLQEVGMPWIIDLKDGLEENKSNPHFDLTDY
                     IRTNAKKAKERGATAVVLYNSSSIEDNLKFNGKDRSEMLSIPVLYVTKEAAKKYFLSA
                     ESSFNLKINVDLADKKRTGHNVVGYLDNGAATTVILGAHFDHLGYGEDGNSMMRTGEK
                     LIHNGADDNASGTATLIELARKLKASKSKNNNYLFIAFSGEELGLYGSKYFTENPTID
                     LKTANYMINMDMVGRLNDSTKALTVGGYGTSPKWGEFYNSSLFKEKQDFLFKYDSSGT
                     GPSDHTSFYRKDIPVLFYFTGLHTDYHKPSDDVERINYNGTASIITHIQSLIEATNNS
                     PKLAFTKTREAQTTSSRTFSVSMGIMPDYTYSGSGMRADGVTEGKPAQKAGMQAGDIV
                     IQLGEHPITSMDSYMKALGKFKKGDKTVVKFKRGSETLERTVEF"
     gene            2922..3371
                     /locus_tag="PK977_06745"
     CDS             2922..3371
                     /locus_tag="PK977_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HRF17845.1"
                     /translation="MAERSNKLILDTKEMTEGFFEDTRLLGIMAPLKDYQFCWHLNNS
                     IGLNFRLNSDLEIPLLRKGRNYFFTVYEYNEPTRFLSHYLYNTKFDGEYLLPEFRHLD
                     FLWLMKGDAVADETMQETIHAIKSFNGVQLVTEIPIEQVKNKENLVF"
     gene            3383..3619
                     /locus_tag="PK977_06750"
     CDS             3383..3619
                     /locus_tag="PK977_06750"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRF17846.1"
                     /translation="MWQEKDNTLYRKFQFADFSEAFAFMTRVAIEAEKMNHHPLWINV
                     WNTVEIWLSTHDAGNIVTEKDRKLAAKIDALFPH"
     gene            3600..4559
                     /locus_tag="PK977_06755"
     CDS             3600..4559
                     /locus_tag="PK977_06755"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRF17847.1"
                     /translation="MHYSLIKELVSMPSATTILMIRPAAFGFNPETANDNLFQVKTGV
                     SNDALQLNALAEFDNMVSQLRKNGVDVLVIEDTATPIKPDAVFPNNWLSTSPNGKLFV
                     YPMYAPSRRAEKRDDIIKTLKKQFKVKDFQDWTEFEAEGRFLEGTGSMVMDHENKMIY
                     AAISERTSIAVLERFAAENHYQAIVFLATDKDGFPVYHTNVVMTLGSTFCVLCEEAIE
                     EEWELIAVRQLLESTHHSIIPITREQMHSFAGNMLEVKNNKGEHLLVLSQTAYDSLRK
                     EQKQMLEAYAKLLPIAIPTIEQAGGGSVRCMMAEVFLEKRRKD"
     gene            complement(4564..>4851)
                     /locus_tag="PK977_06760"
     CDS             complement(4564..>4851)
                     /locus_tag="PK977_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17848.1"
                     /translation="RKNIIVWGDNGHLCRPFVSQFKKDSTYIMALDEGSEKWGQERES
                     RNDYSISNCGAFWLNADLFQQKIVGDINSTDRKTQTLALKEFKSELLNSFR"
     assembly_gap    4852..4910
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5171..6232)
                     /gene="selD"
                     /locus_tag="PK977_06765"
     CDS             complement(5171..6232)
                     /gene="selD"
                     /locus_tag="PK977_06765"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRF17849.1"
                     /translation="MTIDSLLPTPNPIRLTQYSHGAGCGCKISPKVLDEILKSNISLP
                     DNDKLLVGNHSKDDAAVYDIGNGTALISTTDFFMPIVDDPFDFGKIAAANSISDVYAM
                     GGKPLMAIAILGWPVEKIPADIAQRVVEGGRTICAEAGIPLAGGHSIDSPEPIFGLAV
                     TGIVPKENLKQNNTAQEGDYLFLTKPLGVGILATAQKRGLLKEEHLPALISQLTALNK
                     VGEELGKINGVTAMTDVTGFGLLGHLMEMAEGSGLGAEIYYDKLPVAAGVREYIAQRI
                     FPDATTRNWSSYGDKVKFEKGVNVMEAFTLLPDPQTNGGLLVSAKEEAIEAVKAIIGK
                     EILPVGRMIAAGEKRVWVM"
     gene            complement(6303..8099)
                     /locus_tag="PK977_06770"
     CDS             complement(6303..8099)
                     /locus_tag="PK977_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17850.1"
                     /translation="MKPVCNIKNLRLTALLLITPLFFFGQSLTGLWVGSVHNDSATVR
                     KDQSFEIALTEYRGKVYGYSRSEFIVNDTLYYIVKRVKGTIEGDVCEVTDDEIIAFNF
                     RGKLDKGIKVTSTFRRNKGDSTWYLAGTWKTNATKRYYSITGKVGLEEEKDLTASKIF
                     PHLEELKLANDVAFYKEREEGAPIVKIAKPEKIKTEYSTVPEISSSATELVVAQPQFE
                     RAESIPVVKKAESDLPKTDTKNIPSSTIDIKPGAVEKKLAVSKPPAEEAITNKEVAGT
                     AGDTKTPDAANVAANNNPTAGPADRNTTISKTNSPENVKSSANKQQATIAQPVANNAT
                     KQVTGDNKIATQSTTAVPDNKVNDVVANNTGTPTTVKTNQPTSVNATKASTVVAKHEV
                     AAGKPATKPTTGTDKSTVAPKENNAVTKTTTAASLTQPITSPAVNQAGKNEVTDEKTL
                     AGVKAPEKRETASTPVIERVPIPAVDIIQKASLIAGRKSEFSQVVNFKSDSLELALYD
                     NGEIDGDTVSLFLNGEVLMAKQGLKANAIKKTIYITPGNEDFTLVLFAENLGKYPPNT
                     GLLVVHDGEDVYNLRFSSDFQKSTGIVFRRKK"
     gene            complement(8068..9129)
                     /gene="mnmH"
                     /locus_tag="PK977_06775"
     CDS             complement(8068..9129)
                     /gene="mnmH"
                     /locus_tag="PK977_06775"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRF17851.1"
                     /translation="MAVEKIDIGQFLELAKDHPVFDVRSPGEYKHAHIPGAYSLPLFN
                     DEERKVVGTTYKQESREQAIKAGLDFFGPKMRKMVEEVESIVSSQQPKDSKLILVHCW
                     RGGMRSAAVAWLLDLYGFRVFTLTGGYKKYRNYILDTFKMPFRFNILGGFTGSGKTEL
                     LKSLEENGEKVIDLEGIAQHKGSAFGNIGMPEQPSQEMFENILGNKLLDAGVGSAVWL
                     EDESQRIGHVNIPNDLWATMRRSPVFYLDIPFAERLKHIVQEYGQLDTERMIGAIERI
                     SQKLGPQNAKSAILWLKEGKITESFEILLQYYDKFYFRSLHNREALDTLLHTIKCKSV
                     STENANILVRETSLQHQKS"
     gene            complement(9209..>9476)
                     /locus_tag="PK977_06780"
     CDS             complement(9209..>9476)
                     /locus_tag="PK977_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRF17852.1"
                     /translation="IFEEFKGTGNMELQLDRRLSNKRIFPAIDIVASSTRRDDLLLEK
                     DVLQRMNLLRVYLTDMNTEEAMRELQNRMKGTKDNEEFLASMNR"
     assembly_gap    9477..9557
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9558..10841)
                     /gene="rho"
                     /locus_tag="PK977_06785"
     CDS             complement(<9558..10841)
                     /gene="rho"
                     /locus_tag="PK977_06785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRF17853.1"
                     /translation="MYDILQLNDMLVPELLDIAEQLKIPNAKKLSKQDLVYKILDSQA
                     IAGAKGAKPSDDGKRKRIVKTSTSTGGSEEAVVESGDEEKTNTKKPHMAVKKEVPVKR
                     GRKKTDEKEVVADVAEQSAPAAEPEMDSKTAQLAEAILNEASQQTSENTGQGAPNKIY
                     PQRREQQFNVEFDGVILGEGVLEMMPDGYGFLRSSDYNYLSSPDDVYVSPSQIKLFGL
                     KTGDTVHGAVRPPKEGEKYFALLKVDTINGKSPEEVRDRVPFDYLTPLFPYEKLNLFT
                     TASDYSTRIMDLFTPIGKGQRGLIVAQPKTGKTMLLKAVANAIAANHPECYLMVVLVD
                     ERPEEVTDMERSIKGEVIASTFDEPAEKHVKVSTIALQKAKRLVECGHDVVILLDSIT
                     RLARAHNTVAPASGKVLSGGVEANAMQKPTQFFGAA"
     gene            complement(11032..>11665)
                     /locus_tag="PK977_06790"
     CDS             complement(11032..>11665)
                     /locus_tag="PK977_06790"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HRF17854.1"
                     /translation="HPDHKVVSYINCSAGIKALSDVICTSSNAKFIVESFPKDQPLIF
                     APDKNLGAYINKVTGRNMVLWNGACMVHEIFSLEKITKLKVRHPEAKLIAHPECEEPI
                     LRIADYIGSTTGLLKYTQTDSAQEYIVATETGILHQMMKASPEKTFIPAPPNNSCACN
                     DCPHMKLNTLEKLYLCMEYETPEIIMEEELRLAAKKPIDRMLEISKAAGL"
BASE COUNT         3029 a         2628 c         2445 g         3413 t
ORIGIN      
        1 cgccagccag aatacacttt gaaacaggag gaacaaaacg gccagcagga tcaaatagcc
       61 ccagcgccgg tgtaaaaaaa tattgtcaag cctttcggta aataatgatt tctgtagcgg
      121 gtcgggttct gatactgcct gctgcatgat ttgccggatg cggctatagc gttgtaagat
      181 ctcttctgcc tgggttttgg taggattaaa attatttttt acttcaatgc tctctattag
      241 ctcctgttcc tttccgctca gtacaaacga ttcgtggtta ataagataat gtatagctgt
      301 ataatcgctg agttccggaa aatcctgttt cagctcatta atggcatcgg cagcaaaaga
      361 tttgttatcg atgaaatctc ttgccggtac tttatatgcc tgttgggcnn nnnnnnnngt
      421 aagtgcaatg gccttcttta attcaggaat gcctttgttc tttaacggat tcaccgccac
      481 taccggaacc cccaactccc tttccagtgc cggtatatct attttgattc ctttgcgctt
      541 ggccatgtcc atcattgtaa gggcaataac gaccggaact tttaaatcaa tcagctgggt
      601 gcagaaaagc aggtttcgtt ttaagttgct ggcatcggcc actgctacaa ttacatcggc
      661 cctcatctct ctgtcctggc ccagcaagac cttatacgtc acccattcat ccattctctt
      721 cgggtaaaga ctatagctgc ccggcaggtc tatgatttca gccgtttggg atggagatat
      781 ttgagacgaa cccgttttct tgtctactgt tacgccggga aagttgccca ctttttgatt
      841 aagccctgtc agacaattga acagggagct tttgccgcta ttggggttac caaccaatgc
      901 aatatgtaag cggttgtttt tcacttcaac aagcactaac ccgcccggag gaggaaagtt
      961 tgcaaaagta atcctggcgg gtggattgac cttacgaaat acggtgttta acccctgcta
     1021 tcgggaaaag aatgacgtaa aaatgatgct ggaaaaaaag gttacggccg ggcattaata
     1081 cctttgctgc tcaattctaa aaaatatggt atcgtcatta ttgagagtgt ctgttattgc
     1141 gttgttactg gtagcctgcg ccgcacccaa acaggcttcc aaaacagata agcagcttat
     1201 tgaaaacctg aaaacacata tcagcttcct cgctgatgat aaactagaag gtcgccggac
     1261 cggcagcgcc ggagaacagc tggcgatgga atacatcagc agccagttta aaaccattgg
     1321 tctttctccg aagggaacca gcggatttta tcagcccttt gaagtaaatg acggcaaaat
     1381 catgaagctt tcttcgctat tcatgattaa cggagacggg ctggtagaag agagagatta
     1441 ttttgctttt cctttttctc ccaatacaag cattgatgcg tttgtttccc cttcgctgca
     1501 ggaagtaggg atgccatgga tcattgacct gaaagacggg ctggaagaaa acaaaagcaa
     1561 cccgcatttt gacctgactg attatatccg caccaacgcc aaaaaagcaa aagaacgggg
     1621 tgctacagct gtggttttat ataacagctc ttcgattgag gataatctta agtttaatgg
     1681 caaggaccgc tcagaaatgc tttccatccc ggttttgtat gtgacaaaag aggcagcaaa
     1741 aaagtatttt ctttctgctg aatcatcttt taaccttaaa ataaatgttg atctcgctga
     1801 taagaaaaga acaggtcata acgtagtcgg atatttggat aatggtgccg ctactacggt
     1861 gatactcggt gctcatttcg atcacctggg ttatggtgaa gacggaaact ctatgatgcg
     1921 gacaggggag aagcttatcc acaacggggc cgatgataat gccagcggta ctgctacgtt
     1981 gattgaattg gccaggaaac tgaaagcatc aaaatctaaa aacaacaatt acctctttat
     2041 tgcattctca ggggaagagc tgggattgta tggttcaaag tattttaccg agaacccaac
     2101 gattgatctg aaaacagcca actatatgat caatatggac atggtgggcc ggctgaatga
     2161 cagcaccaaa gcattgacag tgggcggtta tggcacttcg ccaaaatggg gagaatttta
     2221 taactcctct ctttttaaag aaaagcagga cttccttttc aaatacgatt ccagcggaac
     2281 cggtccgagt gatcatactt ctttttacag aaaagacatt cctgttcttt tttattttac
     2341 cggccttcac acggattatc acaaaccttc cgatgatgtc gaacgaatca actacaatgg
     2401 aacagcttcc ataattaccc acatacagtc gcttattgaa gcaacgaata actcaccaaa
     2461 gctggctttc accaaaacaa gagaggcgca aaccacctcc agcagaacgt ttagtgtatc
     2521 aatgggcatc atgcctgatt atacttattc aggatcagga atgagagcag atggcgttac
     2581 agagggcaag ccggcacaaa aagccggaat gcaggcaggc gatatcgtta tccagttggg
     2641 cgaacacccc attacatcta tggacagcta tatgaaggca ttgggtaaat tcaagaaagg
     2701 agacaagacc gtagtgaaat ttaaaagagg aagcgaaacg ctggaacgta ctgttgagtt
     2761 ctagtaatgg cacacagatg acacagatta aactgattga attttagcaa aagccggaat
     2821 tcaaaagaaa gatttttaca aacgataaga aaaaatccgt ttagacccgt actatctgtt
     2881 taatccgtgt gctattttgt aacttagcct atatttcatg tatggctgaa cgcagcaata
     2941 agctgatcct tgatacaaag gaaatgactg aaggtttttt tgaggatacc cggttgctgg
     3001 gcatcatggc acctctgaaa gactaccagt tttgctggca cctgaataac tctatcggcc
     3061 ttaattttcg tcttaacagt gatcttgaaa ttccgttgct ccgcaaggga agaaattatt
     3121 tctttactgt gtacgaatac aatgagccca cccggttctt gtcgcactat ttatacaata
     3181 caaagtttga tggtgaatac ctgttgcccg aattcaggca cctcgatttt ctctggctga
     3241 tgaaaggtga tgcggtggcg gatgaaacaa tgcaggaaac catccacgcc attaaaagct
     3301 tcaacggggt acagctggta acggagattc cgattgaaca ggtgaagaac aaagaaaatc
     3361 ttgtgtttta aatactgttt ctatgtggca ggaaaaagac aatacattat accgcaaatt
     3421 tcagttcgct gatttcagcg aagcctttgc atttatgaca agagtggcga tagaagcaga
     3481 aaaaatgaat caccatccgc tgtggatcaa tgtttggaat acagtagaga tctggctcag
     3541 cacacatgat gcaggaaata tcgtaacaga gaaagacagg aaactggcag ctaaaataga
     3601 tgcactattc cctcattaaa gagttggtaa gtatgccatc cgctactacc atattaatga
     3661 ttcgtcccgc cgcttttgga tttaatcccg aaacagcaaa cgataatctc ttccaggtaa
     3721 aaacaggtgt aagcaatgat gcattacagc taaacgccct tgctgaattt gacaacatgg
     3781 tcagccagct tcgtaaaaac ggagtggatg tcctggtaat tgaagacacg gcaacgccaa
     3841 taaaaccaga tgccgtcttt cccaataact ggttaagtac ttcgcccaac ggaaaacttt
     3901 ttgtgtaccc gatgtatgcg cccagccgcc gggcagaaaa aagagacgac attatcaaaa
     3961 cgctgaaaaa acaatttaaa gtaaaagatt tccaggactg gaccgaattt gaagcggaag
     4021 gcaggttttt ggaaggaaca ggaagcatgg taatggatca tgagaataaa atgatctatg
     4081 cagctatatc agaaagaaca agtattgctg tgctggaacg ctttgctgca gaaaaccatt
     4141 accaggccat tgtcttcctg gcgaccgaca aagacggttt tcctgtttat cataccaatg
     4201 tggtaatgac actgggttca actttttgtg tgctctgcga agaagccatt gaagaagagt
     4261 gggagctgat cgctgtaagg caactgctgg aatcaaccca tcatagcatc atccccatca
     4321 ccagggaaca aatgcattcc tttgcgggta atatgctgga ggtaaaaaat aacaaaggcg
     4381 aacacctgct ggtgctcagc cagaccgcct atgattcgct gcgcaaagaa cagaaacaaa
     4441 tgctggaggc ctatgctaaa ctattgccca ttgccatccc aacgattgag caggccggcg
     4501 gcggcagtgt gcggtgtatg atggcagaag tgtttttgga gaagaggaga aaggattaat
     4561 tgattagcgg aagctattaa gtagctctga tttaaattct tttaaagcga gtgtttgagt
     4621 ttttctgtct gtgctattaa tatcacctac tattttctgt tggaaaaggt cagcattaag
     4681 ccaaaaagcg ccacaattgc taatcgaata atcgttcctg ctttctcttt cttgacccca
     4741 tttttcactg ccttcatcaa gtgccattat gtaagtgctg tcttttttaa actgtgatac
     4801 aaaaggtctg cataagtgtc cattatctcc ccatacaata atattctttc tnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atagccattg
     4921 gtgttttaga aatttctgaa actaccggat tatcctttac tttaaccatc acaacaagtt
     4981 ttgactgttt agacattgcg ataaatggac cattataacc gcaatcacat gccattgcct
     5041 ttgaagaaag aatcaataaa aatataacaa gaacctgtct catattatta tttgcagtgc
     5101 tgcacccttg cttaaattac ttctaacctc taaatgcaac tcaataatca cccgctgcca
     5161 atactaatct ttacatcacc caaacccttt tctctccagc agctatcatt ctgcctactg
     5221 gcaaaatttc ttttccaata atagccttta cggcttcaat agcttcttct tttgcactca
     5281 ccagcaatcc gccattcgtt tgcgggtcgg gcagtaaggt aaatgcttcc atcacattta
     5341 cacccttctc aaactttact ttatcaccgt aactgctcca gttacgggtg gtggcatcag
     5401 gaaaaatgcg ctgggcaatg tattctctta ccccggccgc taccggtagt ttgtcataat
     5461 atatttctgc accgagtccg ctgccttcgg ccatttccat caggtgcccg agcaaaccaa
     5521 aaccggttac atcagtcatg gcggttacac catttatctt acccagctct tcgcccactt
     5581 tattcaatgc cgttagctgg ctgatgagtg ccggcaggtg ttcttctttc agcaatcctc
     5641 ttttttgtgc cgtggccagg ataccaacgc ccagcggttt ggtcaaaaac aaataatcgc
     5701 cttcctgtgc tgtattgttt tgcttaaggt tttctttggg tacgatgccc gtcaccgcca
     5761 aaccaaatat cggttcgggt gaatcaatgc tgtggccacc tgccaatgga atgcctgctt
     5821 ctgcacaaat ggtccttccg ccttccacca ctcgttgtgc aatgtcagcg ggtatctttt
     5881 caaccggcca gcccaggata gcaatcgcca tcagcggttt tccacccatg gcatatacat
     5941 cgctgatgga gttggcggca gcaatttttc caaaatcaaa cggatcatct acgatgggca
     6001 taaagaaatc ggtagtagaa ataagcgccg taccgtttcc tatatcatat acagccgcat
     6061 catctttgct gtgattgcct actaatagct tgtcgttgtc aggtaacgag atattgcttt
     6121 tcagaatttc atccagcacc ttcggtgaaa tcttgcaacc gcagccggcg ccgtgtgagt
     6181 attgggtgag acgaataggg ttaggagtcg ggagtagaga gtcgatagtc attctgcaaa
     6241 attcggtttg ttgttgcaga caacccgctt aattgaaaaa aatcagtgca tttcgttgtc
     6301 ccctatttct ttctccgaaa cactatccct gtgctcttct ggaaatcaga actgaaacgg
     6361 agattgtata cgtcttctcc atcatgcact accagcagcc cggtattggg cggatatttt
     6421 cccaggttct cggcaaacaa aaccagtgta aaatcttcat tgcccggtgt gatgtagatg
     6481 gttttcttta tggcgttggc cttgagtccc tgcttggcca tcagcacttc gccattcagg
     6541 aacaggctta ctgtatcgcc atcaatttct ccgttatcat acaaggccag ctccaggctg
     6601 tctgatttaa aattcaccac ctgtgagaat tcagattttc tgccggcgat caaagaagcc
     6661 ttttgaataa tatccactgc cggtatgggt actcgttcaa ttacaggggt actggctgtt
     6721 tctctttttt ccggtgcttt aacacctgcc agtgtttttt cgtccgtcac ttcattctta
     6781 cccgcctggt tgacagcagg tgatgtgatg ggctgggtta agctggcagc agttgttgtt
     6841 ttagtaacag cattgttttc ttttggagca accgttgatt tgtctgtacc agttgttggc
     6901 ttcgtagcag gtttgcctgc agcgacttca tgtttagcaa caacagtgct ggcttttgtt
     6961 gcattaacgg atgtcggctg attcgtttta actgttgttg gtgttccggt attattagcc
     7021 accacatcat ttaccttgtt gtcaggaaca gctgttgttg attgagtagc tatcttatta
     7081 tccccggtta cctgctttgt agcgttgttt gcaacaggct gagcaattgt tgcctgttgc
     7141 ttgttcgctg aactttttac attctcaggg ctgtttgttt tactgattgt tgtgttccta
     7201 tctgcaggac cagcagtagg gttattattc gcagcaacat ttgcagcatc gggtgttttt
     7261 gtatcaccgg ctgttccagc cacttcttta ttcgttattg cttcttcggc gggtggctta
     7321 cttactgcca actttttttc aacagctccg ggttttatat caatggttga agaaggaata
     7381 ttttttgtat ccgttttggg cagatcgctt tccgcctttt tcactactgg tattgattct
     7441 gctctttcaa attgtggctg ggcaacaacc agctctgtgg ctgatgaaga aatttccggt
     7501 acggtagaat attccgtttt tattttctcc ggctttgcaa tttttacgat cggcgcacct
     7561 tcttctcttt ccttgtaaaa agctacgtca ttggccagtt tcagttcttc caaatgggga
     7621 aatatttttg aggccgtcag gtctttttct tcttccagtc ctacttttcc ggtgatggag
     7681 taatatcgtt ttgttgcgtt ggtcttccag gttccggcaa ggtaccaggt gctgtcgccc
     7741 ttgttgcgac gaaaagtgct ggttactttt attcctttat ccagtttgcc ccggaaatta
     7801 aatgcaatga tctcatcatc ggtcacctca catacatctc cttcaatagt gcccttaact
     7861 ctttttacta tgtaataaag cgtatcgttg acaatgaatt cactccggga gtaaccgtaa
     7921 actttgccgc ggtactcggt cagggcaatt tcgaatgact ggtcttttcg gaccgtggca
     7981 ctgtcgttat gcacggagcc aacccataat ccggtcaggc tttgtccaaa gaaaaaaaga
     8041 ggggtaatga gcagcagggc ggtaagccta agatttttga tgttgcagac tggtttcacg
     8101 aacgaggata ttggcatttt cagtggatac ggatttacac tttatcgtat gtaataacgt
     8161 atccagcgcc tcccgattgt gtaaagacct gaaataaaac ttatcgtagt actggagcaa
     8221 aatctcgaaa ctttcggtga ttttgccctc ttttagccac aaaatggccg attttgcgtt
     8281 ctgcggaccc agtttttggc taattcgctc aatagccccg atcattcttt cggtgtccag
     8341 ttggccatat tcctgcacaa tatgtttgag cctttcggca aaagggatgt ccaggtagaa
     8401 taccggcgac cgccgcatgg tggcccacag gtcgttagga atatttacat ggccgatacg
     8461 ctgtgattcg tcttcaagcc agacggctga cccgacaccc gcatccagta acttattgcc
     8521 cagtatgttc tcaaacatct cctggctggg ttgttccggc atcccgatat ttccaaaagc
     8581 agaacccttg tgttgggcaa ttccttccag gtcgatcact ttttcaccat tttcctccag
     8641 cgattttaat aattccgttt tacccgaacc ggtgaaaccg ccaaggatgt tgaagcgaaa
     8701 cggcatttta aaagtatcca gtatatagtt acggtacttc ttatacccgc cggttagtgt
     8761 aaaaaccctg aacccataaa ggtccaataa ccaggcaaca gctgcactgc gcataccgcc
     8821 tctccagcag tgaacgagga tgagtttgga gtctttcggc tgttgactac tgactataga
     8881 ctcaacctcc tccaccatct tccgcatctt cggtccgaaa aaatcaagtc cggccttaat
     8941 agcttgttca cggctttctt gtttgtaagt agtacccact acttttcttt cttcatcgtt
     9001 aaacaggggt aaggaataag cgccgggtat atgtgcatgt ttgtattcac ccgggcttct
     9061 tacatcaaaa accgggtgat cttttgccaa ttctaaaaac tggccgatat ctattttttc
     9121 aaccgccatt atcaattgta aaaataaaaa ggctgccgaa aaacagcagc ccttattatg
     9181 ctaatcattt taatgacttg aatccaggtc acctgttcat actagccaaa aactcttcat
     9241 tgtctttggt tcctttcatc cggttctgca attctctcat cgcttcttcg gtattcatat
     9301 ccgtcaggta aacacggagc aggttcatcc gctgcagtac atctttctcc agcaacaaat
     9361 catcacgcct tgtactggat gccacaatat caatagcagg gaagatccgt ttgttagaca
     9421 atcttctgtc gagctgcaat tccatattac ccgttccctt gaattcttca aatatcnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnggc agcgccaaag aattgtgttg gtttctgcat cgcattggct
     9601 tccacaccgc cggataatac tttaccggat gcaggagcta ctgtattatg tgcccttgcc
     9661 aaacgggtaa tcgaatcaag aaggatcact acatcatgtc cgcactctac cagtctcttt
     9721 gctttttgca aagcaatagt agaaactttc acatgttttt cagctggttc atcaaaagta
     9781 gaagcaatta cttctccttt tatactgcgt tccatatcag ttacctcctc cggacgttca
     9841 tccaccagca ctaccatcag gtaacattca ggatgattgg ctgcaatggc atttgccact
     9901 gctttcagca acatcgtctt accggtttta ggctgtgcca caatcaatcc ccgctgtcct
     9961 ttacctatag gtgtgaacag gtccatgata cgggttgaat aatcagacgc tgttgtaaat
    10021 aaattcagct tttcataagg gaaaagcggg gtcaggtaat caaagggcac tctgtcacga
    10081 acctcttcag ggctcttgcc attgatcgta tccactttta ataaagcaaa atacttttct
    10141 ccttctttag gcggacgaac agcgccatgc accgtgtcgc cggtctttaa accaaacagt
    10201 tttatttgag aaggggaaac atatacatca tccggtgatg acaaatagtt gtaatcagat
    10261 gaacgaagga atccataacc atcaggcatc atttccaata caccttctcc taatatcaca
    10321 ccgtcaaact ccacattaaa ttgttgttca cggcgctgag gatatatttt attgggtgct
    10381 ccttgtcctg tattttcaga agtctgttgg ctggcctcat ttaaaatagc ttcagccagt
    10441 tgtgctgtct tactatccat ttcgggttct gcagccgggg ctgattgttc agctacatct
    10501 gccactactt ctttctcgtc tgtcttttta cgtccccgct ttaccgggac ttcttttttt
    10561 acagccatat ggggtttttt ggtattggtt ttttcttcgt cgccgctttc cactaccgcc
    10621 tcttcactgc caccggtact ggtactggtt tttacaatgc gctttctttt accatcatca
    10681 ctgggtttag cgcctttagc cccggctatt gcctggctgt caagtatctt ataaacaaga
    10741 tcttgtttgc ttaatttttt tgcgttggga atttttaact gctcagctat atccagcagt
    10801 tcaggaacga gcatgtcgtt cagttggaga atgtcataca tataaaaaac ttgatgttag
    10861 tcacaactcg aagtcaccaa tcttgaaatc tgaaattttg agtttatatt gaaagaaaca
    10921 ggcgggtaaa tgatgaacaa aagcctggga gaactatact tatcagcctc tttccggtgc
    10981 aagtatacag caaaacccgg aaaatcaaaa attttagcct taaatcgctg attacagccc
    11041 cgcagcctta ctgatctcca gcatccggtc gatcggtttt ttggcggcca gcctgagttc
    11101 ctcttccatg ataatctccg gtgtttcata ctccatacac aggtataatt tctccagcgt
    11161 attcagcttc atatgcgggc agtcattaca ggcgcaactg ttattgggcg gcgccggtat
    11221 gaatgttttt tccggggaag ctttcatcat ctggtggagt atacctgtct ctgtggcaac
    11281 aatatattct tgtgctgaat cggtttgtgt gtactttaat aacccggtag tggatcctat
    11341 ataatctgca attcttaata tgggttcttc gcattccggg tgtgcaatca gttttgcctc
    11401 cgggtgacgc acttttagtt tggtgatctt ttccaggctg aatatctcat ggaccataca
    11461 ggcgccattc catagtacca tattcctgcc ggttactttg ttgatataag cacccaaatt
    11521 tttatccggt gcaaagatca atggctggtc tttgggaaaa ctttctacta tgaatttggc
    11581 attactgctg gtgcagatca catcactcaa cgctttgata ccggcgctgc agttgatata
    11641 actcaccact ttatgatccg ggtgc
//
LOCUS       DAORLS010000065        11632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2949_length_11632_cov_1.939017, whole genome shotgun sequence.
ACCESSION   DAORLS010000065 DAORLS010000000
VERSION     DAORLS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2949_length_11632_cov_1.939017"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..886
                     /gene="accD"
                     /locus_tag="PK977_06795"
     CDS             5..886
                     /gene="accD"
                     /locus_tag="PK977_06795"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRF17855.1"
                     /translation="MAGEENNQNETRASWFRRIKKGINTKTSEKKETPEGLWVKCPEC
                     NHICTGSDLKENLSVCPKCNYHHRINSLEYYDILFDEHQYTELFDNIRSKDYLGFTDL
                     KPYQKRLEEIWNKTDLKDSMRVAVGKVGGHDMVISCMDFEFIGGSLGSVMGEKFARAV
                     DYCIEHKMPYMVISKSGGARMMESAFSLMQLAKTSGKLSQLSDAKLPYISLLTDPTFG
                     GISASFGMLGDMNIAEPGALIGFAGPRVIKETIKKDLPEGFQRSEFLLEHGFLDFIVP
                     RKELKEKLAKVISLLKN"
     gene            946..1383
                     /gene="smpB"
                     /locus_tag="PK977_06800"
     CDS             946..1383
                     /gene="smpB"
                     /locus_tag="PK977_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRF17856.1"
                     /translation="MAELKNRQAYYDYFIDVKYDAGIVLLGTEVKSLRQGKASFNDSY
                     CIIHKGEIWVKSMHIAEYSHGTVNNHDPLRDRKLLLEKREIRKIETKLKEKGYTLIPL
                     RIHFNEKNLAKMEIGLARGKKQHDKRDSIRQKDAEREMKRFMK"
     gene            1541..2467
                     /locus_tag="PK977_06805"
     CDS             1541..2467
                     /locus_tag="PK977_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17857.1"
                     /translation="MKRLIIFILLFSFYTAGAQTVFGYWYGNANVKINSSANNYLVEM
                     ILQPEKNYVKGILNYYFKNTYRSIKVQGDYSPSTRQLSLYNIPVVYHASMNNFEVDCN
                     MNLQATLRVAQAGSNLVGAFRALPDYRYTCPDITFNLVLNADISKKDSVLQAISEYKE
                     NYQVWQPGVDDTLVAVNVIPRKVINYVTEKQYTERENITIKEIEVESDSIRVDIYDNG
                     EIDGDIVSIFYNQKLILYNQKLTHKSVKIGLPLDSTLAANELSMFAENLGLIPPNTAL
                     MVIQDGKNRHEVRVSSNLEKNATIKIRRKKKQ"
     gene            complement(2468..2728)
                     /locus_tag="PK977_06810"
     CDS             complement(2468..2728)
                     /locus_tag="PK977_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRF17858.1"
                     /translation="MTLDHFIAIVVIGAAAGWLGSFLVKGRGLGLIGNIIVGILGSFV
                     GSWLLKELNVSIGSTPLVSSILTGAIGAFVILFVASFFSGKS"
     gene            complement(2746..5259)
                     /locus_tag="PK977_06815"
     CDS             complement(2746..5259)
                     /locus_tag="PK977_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRF17859.1"
                     /translation="MSAAKNKLTEVQNNDSGLHGQYKTWFLDYASYVILERAVPAIED
                     GLKPVQRRILHAMKEMDDGRFNKVANIIGQAMQYHPHGDASIGDALVNMGQKDLLIDT
                     QGNWGDVRTGDDAAAARYIEARLSKFALDVAFNAKTTAWQLSYDGRKNEPVTLPMKFP
                     LLLAQGADGIAVGLSTKILPHNFCELIDAAVKYLRGKRFELYPDFMTGGMIDVADYNQ
                     GKRGGKVKVRAHIEEVDKKTLAIKSVPYGVTTTQLMESIVKANDQGKIKIKKVTDNTA
                     AEVEIQVDLAPGISPDITIDALYKFTDCEVSISPNACVIVEQKPQFLGVQELLKLSVD
                     KTKDLLEQELKIKLNELQEKWHYTSLEKIFFEEKIYKELEKKHETWDKVLDAIDKAFI
                     PFKKQLKRDITKEDIIKLTEKPVRRIYKLDIDELNDQIKGLEAEIKQVKHDLANLVDF
                     AVAYYENLLKKYGKGRERKTEIRQFEVIEAKNVAIANTKLYVNRAEGFIGTGLKKDEL
                     LFECSDLDDIIVFAKRGIMKVVKVSDKTFIGKDILHAAVFQKNDERTTYNMIYADGAT
                     GISYAKRFNVTGVTRDKEYDLTKGHEKSKVHYFTANPNGEAEVVKIVLGNCSARIKEF
                     DYYFEQLEIKGRGSMGNQVTKYPIKSVKFKEAGRSTIAGKKIWYDDQFGRLNLEEKGS
                     YLGVFDAEDKILVVYSDGNYEITDQELTQKLDSEKVIFIEKFNPEKIVTAVYADMEKK
                     QFMVKRFKIETTTLKNKFMFIKEGDGNYVEAVTTTEEPVLAMQQGRGAQIRKGKLKIN
                     KLAEVTGWKTVGTKLADYSKSTEMEWIKPKEEDNQPELF"
     gene            complement(5263..5616)
                     /locus_tag="PK977_06820"
     CDS             complement(5263..5616)
                     /locus_tag="PK977_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HRF17860.1"
                     /translation="MKNLLKLEEAAMFGLSLYLLGSMNAEWWLYLLLLLGPDISMLGY
                     LGGNKTGAICYNIFHHKGVAIAVFMAGLLLPNTLFEVTGIILYGHSSMDRMFGYGLKT
                     EEGFKYTHLGIIGKK"
     gene            complement(5613..6470)
                     /locus_tag="PK977_06825"
     CDS             complement(5613..6470)
                     /locus_tag="PK977_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HRF17861.1"
                     /translation="MIHIVFNESEVSLMKQVIEIDEALAGEVVQIKDDFAVGPLAGIE
                     TEEGWQARIEWCRNLLQGSPYGEELAGSFDDRETVTRLKSKLDGNPAEELWIWMGQNQ
                     HDVMGYYWLMPQFKDYQSRVMILYLNNLPFINEKGQIFYPSWLHEIQPKEFIKAKKLA
                     RPITLSEFEIDPDEWRKIAEENAIVRVLEGGKKIVGKDESFYDAEILKNVTGEWQKAT
                     RVLTNTLHRMKIKTGDVFIMWRMKHLVNEGKIEVTGDMAKAWKDFDVKLAGAKQQETV
                     VTEEIPNQS"
     gene            complement(6472..7260)
                     /locus_tag="PK977_06830"
     CDS             complement(6472..7260)
                     /locus_tag="PK977_06830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF17862.1"
                     /translation="MFEFHADRKRYFDIQVMNAEKYVIPFIEEKFPVRTGMRVLEIGC
                     GEGGVLKAFVNKGCEGLGVELDTPRIDDALKFLPDDVAAGKIRFVAKDIYLVDIEKDF
                     SGRFDIIVLKDVIEHIHDQPKLIGWMKNFLNPGGIVFFGFPPWYMPFGGHQQMCRSRL
                     SKLPYIHLLPRSIYRWILKKKKEPVEALMEIRDTGISIERFEKICRKEGYVFLHKRHY
                     LLNPIYEWKFGWKPRKQAGLIKAIPFLRNFLTTCVYYIIQPEKN"
     gene            complement(7266..>8281)
                     /locus_tag="PK977_06835"
     CDS             complement(7266..>8281)
                     /locus_tag="PK977_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRF17863.1"
                     /translation="DIRTGIVAAVSVRVVEPVFESQTKTKLGSVNVDIDGPSMKQFVG
                     DFLAKELDNFLHKNPATAEALKKRIEQSERERKELAGIKKLANERAKKANLHNRKLRD
                     CRYHLDEEAPAKGKEEFVAKQKETTIFITEGDSASGSITKARNVETQAVFSLRGKPLN
                     CFGLTKKVVYENEEFNLLQHALNIEEGLEGLRYNNVVIATDADVDGMHIRLLLMTFFL
                     QFFPDLVKHEKVYVLETPLFRVRNKQETIYCYDEAEKLTAMKKLGGKPEVTRFKGLGE
                     ISPDEFAQFIGPDMRKQLMRLEQGDHIQQLLEYYMGKNTPDRQEFIIENLRIEMDKVE
                     EVA"
     assembly_gap    8282..8500
                     /estimated_length=219
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8501..9158)
                     /locus_tag="PK977_06840"
     CDS             complement(<8501..9158)
                     /locus_tag="PK977_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF17864.1"
                     /translation="MAKEKDTTNLFDYTEDSIKSLDWKEHIRLRPGMYIGKLGDGSSP
                     DDGIYVLVKEVMDNCIDEYTMGYGKTVELSIEGKTVTVRDYGRGIPLGKVVDVVSKIN
                     TGAKYDSKAFQKSVGLNGVGTKAVNALSNYFKVTAIRDGKEKTAEFERGVLVKEHKEA
                     KTGELNGTMVTFIPDESVFKNFKFLPEFLENQIWNYCFLNAGLRILFNGKTFVSKNGL
                     L"
     gene            complement(9314..10042)
                     /locus_tag="PK977_06845"
     CDS             complement(9314..10042)
                     /locus_tag="PK977_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRF17865.1"
                     /translation="MPFAAFAKKFGHKFDTSSPETIAASFNQLLKDVYGRPEQHVLEQ
                     LGIRTMAKAKYTTENVGHYGLGFEHYCHFTSPIRRYPDIQVHRILFDVLNDKVNPDKK
                     LEEKCKHTSERERAAMEAERAANKYKQVQYMKNFLGEEFEGVISGVASFGFWVETVEH
                     KCEGLVSINSLLEYDEFRHIESDYCLAGLRSGRKFRMGDKVWIKVVAANLTKRQLDYE
                     WVVAGDLATVNSRKKKEKKGRKKE"
     gene            complement(9952..11340)
                     /locus_tag="PK977_06850"
     CDS             complement(9952..11340)
                     /locus_tag="PK977_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRF17866.1"
                     /translation="MNLIMGRKNSKKKQKDRQRKSAQLLKGIMDITRSGMGFVTVEGL
                     DADIMIRPADFNTALHGDTVQVAVKEIKSGGRRMQGEIKKVIQRKRTEFIGHLQMNKG
                     FAFFVAEMDKPIPDIFIPLENINGANDNDRVVVRLLQWESDGKRPMGEVVTVLDPENS
                     NDAAMKEILLDAGFPLEFPEEALEVAARIPDVIPGDEIRRRKDVRDIFTITIDPVDAK
                     DFDDAISFRKLKNGYFEIGIHIADVSHYVEPDNALDKEAYHRSTSVYLPYRVNPMLPE
                     HISNVLCSLRPKEDKLTFSAIFQVNAKAEVKQYWLGKTVIHSDHRFTYEEVQAIIEEK
                     AGLYAEEILILNDIAQKFRKKRFDNGAINFSSQEVRFKLNEKGDPIGITIKESFEAHQ
                     LIEEYMLLANRTVAENIAKIKINSRYHFLTVHMMTRMKKSWCLLPLLPKSSAISLILL
                     HRKPLQLHLTSY"
     gene            complement(11344..>11632)
                     /locus_tag="PK977_06855"
     CDS             complement(11344..>11632)
                     /locus_tag="PK977_06855"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRF17867.1"
                     /translation="KLLEEHSNIYHLLQYSDHHIFTIDDLNEIRKRFDELESEEKIIL
                     TTEKDAVRLQKFSNEIGGLPFFVIPVRHQFLFNEGIIFDRLVRDFIKDFKK"
BASE COUNT         3124 a         2678 c         2055 g         3556 t
ORIGIN      
        1 aagcctggca ggagaagaaa ataatcagaa cgaaacaaga gcaagctggt tcaggcgtat
       61 caaaaaaggg atcaatacca aaacctcaga gaaaaaagaa acacccgagg gcctttgggt
      121 gaaatgcccg gaatgtaacc atatatgtac cgggtcagac ctgaaagaga acctttctgt
      181 ttgccctaaa tgcaattatc atcaccgcat aaacagcctt gagtactacg atattttatt
      241 tgacgagcac caatacactg agttgttcga caatatacgc agcaaggatt atctcggctt
      301 tactgacctg aagccctacc agaaaagact ggaagaaatc tggaataaaa ctgaccttaa
      361 agactccatg cgggtagctg tgggtaaagt aggcggacac gatatggtga tttcatgtat
      421 ggattttgaa ttcatcggcg gatcactggg aagtgtgatg ggtgaaaaat ttgcccgtgc
      481 tgttgattac tgtatcgagc ataaaatgcc ttatatggtc atcagcaaga gcggcggtgc
      541 aagaatgatg gaaagtgcat tttcgctgat gcagctggca aagactagcg gcaagctttc
      601 acaactaagc gatgccaaac ttccttatat atctttattg accgacccga ctttcggcgg
      661 tatctccgct tcctttggta tgctgggcga tatgaatatt gccgagccgg gtgcactgat
      721 cggttttgca ggccccaggg ttattaaaga aactatcaag aaggaccttc cggaaggttt
      781 ccagcgcagt gagttcctgc tggaacatgg tttccttgat ttcatcgtac ccagaaaaga
      841 gttaaaagag aaactggcga aagttatcag tctccttaaa aattaatgaa cacctaaata
      901 catgaaaagt aagaggtatt tttgcctctt atttttattt tatagatggc agaactaaag
      961 aacaggcagg cttattacga ttacttcata gatgtaaaat atgatgcggg catcgttttg
     1021 ctgggtacag aagtaaagtc gctgagacag ggtaaagcca gtttcaatga cagctattgt
     1081 atcattcaca agggagaaat ttgggtgaaa tcaatgcata tcgcagaata ttctcatggt
     1141 acagtaaata accatgatcc gctgcgtgac cgtaaactgt tacttgagaa aagagaaatc
     1201 cggaaaatag aaacaaagct taaggaaaag ggttataccc ttattccttt acgtattcat
     1261 ttcaatgaaa agaacctcgc caaaatggaa atcgggctgg caaggggtaa aaagcaacac
     1321 gataaacggg acagcatacg tcaaaaagat gcagaaaggg aaatgaaacg ttttatgaaa
     1381 taacagctgt gtacaacttc ctttcttcaa aaactgcatt ttaacgatct tcaaagctta
     1441 tgaaatgagc cataaaattt ttaagtcatc ttagaccacc cggtgataaa attgtatggc
     1501 gaataacatg aggcaaaatt aatacagtta aaatttactc atgaaaaggc tgatcatttt
     1561 tattttactg ttcagttttt atacggccgg tgcacaaacg gtttttggtt attggtatgg
     1621 caacgccaat gtgaaaatca acagctctgc caacaattac ctggtggaaa tgatcctgca
     1681 accggaaaag aattatgtaa aaggcatact caattactat ttcaaaaata cttatcgcag
     1741 tattaaagtt cagggtgatt acagtccttc tacccgtcag ctaagtttat acaatatacc
     1801 ggtagtgtac catgcttcaa tgaacaattt tgaggtagac tgcaatatga acctgcaggc
     1861 taccttgcgg gtagcacaag ccggctcaaa cctggtaggc gctttcaggg cattacctga
     1921 ttatagatac acttgtcctg atattacttt taacctggtg ctgaatgcag atatcagcaa
     1981 aaaagactcc gttttacagg ctataagtga gtataaagaa aactaccagg tgtggcagcc
     2041 gggggttgat gatacactgg tagcagtgaa tgttattcca cgcaaagtca tcaattatgt
     2101 aacagaaaag cagtatacag aaagagaaaa tatcacgatc aaagaaatag aagtggaatc
     2161 agattctata cgggtggata tttatgataa cggtgaaatt gatggtgata ttgtttcgat
     2221 tttttataac cagaaactga tcctctataa tcaaaaactc acccacaaat cagtaaagat
     2281 cggtttaccg ctggacagta cgctggctgc aaatgaactg tcaatgtttg cagaaaacct
     2341 gggcctgatt cctcccaata ctgcattaat ggtcatacaa gatggtaaga accggcatga
     2401 agtccgtgta tcaagcaacc tggaaaagaa tgctactatc aaaataagaa gaaagaaaaa
     2461 gcagtgatca gctctttcct gagaaaaaac ttgctacaaa aaggattaca aaggccccga
     2521 tggcacctgt aagtatagag cttactaaag gagttgaacc aatactaaca tttaattcct
     2581 ttaaaagcca gcttccgaca aaactcccca ggataccgac aataatattt ccgataaggc
     2641 caagccctct tcctttcacc aggaaacttc ccagccagcc tgctgccgca ccaattacta
     2701 ctattgcaat aaagtgatca agcgtcatag ttatattttg gattattaaa agagttcagg
     2761 ttgattatcc tcctcctttg gctttatcca ttccatttct gtagacttgc tgtaatcggc
     2821 taatttcgtg ccaaccgttt tccagccggt cacttccgct aacttattaa tctttagttt
     2881 gcctttcctg atttgtgcgc ccctgccctg ctgcatagcc aatacaggct cttccgtcgt
     2941 cgttacagct tctacatagt taccatcgcc ttctttgatg aacatgaatt tatttttcag
     3001 ggtggtcgtt tcaattttga aacgcttcac catgaattgt ttcttttcca tgtcagcata
     3061 tacagccgta actatttttt ccgggttaaa tttttcgatg aatatcactt tttcagaatc
     3121 cagtttctgg gtcagttcct ggtcagtgat ctcgtaatta ccgtcactgt aaacaaccag
     3181 tattttatct tctgcatcaa acacgccgag ataagaaccc ttttcttcca ggttcaggcg
     3241 gccaaactgg tcatcatacc atattttctt tccggctatg gtagaacgac cggcttcttt
     3301 aaacttaaca gacttgatag gatacttggt tacctggttg cccatgcttc ccctgccctt
     3361 aatctccagt tgttcaaagt aataatcaaa ctccttgata cgggcagaac aattgcctaa
     3421 tacaattttt actacttcag cttcaccatt cggattcgcc gtgaagtaat ggacttttga
     3481 tttctcatgg cctttggtga gatcgtattc tttatccctc gttacgccgg ttacattaaa
     3541 tctttttgca taactgatac ctgtagctcc atccgcataa atcatattgt aagttgtcct
     3601 ttcatcgttc ttctggaaaa cagctgcatg cagaatatct tttccgataa aggttttatc
     3661 ggaaactttt accactttca ttattcctct tttggcaaaa acgataatgt catccaggtc
     3721 agaacactca aataacaatt catctttctt taaacctgta ccgatgaatc cctcagccct
     3781 attcacatat aacttggtgt tagcaatggc tacattcttg gcttcaatca cctcaaactg
     3841 cctgatttct gtttttcttt ccctgccttt tccgtatttt tttaacaggt tctcataata
     3901 agccaccgca aaatcaacaa ggttggccag atcatgcttt acctgtttta tttcagcttc
     3961 cagtcctttg atctgatcat ttaattcatc gatatccagt ttatagatac ggcgaaccgg
     4021 tttctccgtc agtttaatga tgtcttcctt tgtaatatct cttttcagtt gtttcttgaa
     4081 aggaataaag gccttatcga ttgcatccag taccttatcc caggtttcat gtttcttctc
     4141 cagttccttg tatatctttt cttcaaagaa tatcttttct agcgaggtgt aatgccattt
     4201 ttcctgcagt tcattcagtt tgatcttcag ctcctgctcc agcagatctt ttgttttatc
     4261 tactgacaac ttgagcaatt cctgtacacc gagaaactgt ggtttttgtt ctacgataac
     4321 acaggcattg ggagaaatag aaacctcgca gtcggtgaac ttgtacaatg catcgatcgt
     4381 aatgtcggga gaaataccgg gagccagatc aacctgtatt tcaacttcgg cggctgtgtt
     4441 gtctgttact tttttgatct tgatcttacc ctggtcattg gctttcacaa tagattccat
     4501 taactgggtg gtagttacgc catacggcac actcttgata gcaagtgttt ttttatctac
     4561 ttcctctata tgagccctta cttttacttt tccgcccctt tttccctggt tataatccgc
     4621 cacatcgatc ataccaccgg tcatgaaatc cggatagagt tcaaaacgtt ttccccgcag
     4681 gtatttaacc gcagcatcaa tcagttcaca gaagttgtgt ggcaggatct tcgttgacaa
     4741 tcccacagca ataccgtcag caccctgcgc cagcaacaga gggaatttca ttggcagggt
     4801 taccggttca ttttttctgc catcataact taattgccat gctgtggtct ttgcattgaa
     4861 agcaacatcc agtgcaaatt tgctaagtct tgcttctata taacgggcag ccgctgcatc
     4921 gtcaccggtt ctcacatcac cccagttacc ctgtgtatcg atgagtaaat ctttttgccc
     4981 catattcacc aaagcatccc cgatggaagc atcaccatgc ggatggtact gcatagcctg
     5041 gccgataatg ttggccactt tattgaagcg tccgtcatcc atttccttca tagcatgcag
     5101 tatgcggcgc tgcaccggct ttaagccatc ttcaatagcc ggaacagccc tttctaaaat
     5161 tacataggat gcatagtcca ggaaccaggt cttatactgg ccatgtaaac cgctgtcatt
     5221 attttgaact tctgtaagtt tgttctttgc tgcactcatg acttatttct ttccgattat
     5281 tcctaaatgt gtgtatttga atccctcttc agtttttaaa ccgtatccaa acatcctgtc
     5341 cattgaagaa tgtccatata aaatgattcc ggtaacttca aataaagtat ttggcagtaa
     5401 caggccggcc ataaaaacag cgatagctac tcctttgtga tgaaaaatat tgtagcagat
     5461 tgctcctgtt ttatttccac caaggtatcc cagcatactg atatccggcc ccaatagtaa
     5521 caaaaggtac aaccaccatt ctgcattcat cgaacccaat aaataaaggc tcaggccaaa
     5581 catggcggct tcttccaatt ttagcaggtt cttcatgact gattgggtat ttcttctgtt
     5641 actactgtct cctgttgctt tgccccggca agtttcacat caaagtcttt ccaggctttt
     5701 gccatatcac ctgttacttc aattttacct tcattcacca ggtgcttcat cctccacatt
     5761 ataaatacat cccctgtctt gattttcatg cggtgcaagg tattggtcag cacccgggtg
     5821 gctttttgcc attcgccggt tacatttttg agaatttctg catcgtaaaa actttcatct
     5881 ttaccaacaa tcttcttacc tccttccaac actcgtacta ttgcattttc ttctgcaatt
     5941 tttctccact catcagggtc aatctcaaac tcactcagtg tgatgggacg ggccagtttt
     6001 ttagccttaa tgaattcctt cggctgaatt tcatgcagcc aggaaggata aaatatctgt
     6061 cccttttcat tgataaaagg aaggttgttc aggtaaagaa tcataacccg gctctggtag
     6121 tctttgaatt gaggcatcag ccagtaatat cccattacat catgctgatt ttgtcccatc
     6181 catatccata attcttcagc aggattccca tccagttttg actttaatcg tgtcaccgtt
     6241 tccctgtcat cgaaacttcc ggccagttct tcaccataag gagatccctg caataaattc
     6301 ctgcaccatt caattctagc ctgccatcct tcttctgttt caatgccggc caggggaccg
     6361 acggcaaaat catcttttat ctggactact tcaccagcca gtgcctcatc tatttcaatc
     6421 acctgcttca tcaggctaac ttcactttca ttaaatacaa tatgaatcat tttagttctt
     6481 ttctggttga ataatatagt aaacacatgt tgtcagaaaa ttccgtaaga agggaatcgc
     6541 ttttatcagc cctgcctgct ttctcggctt ccagccaaat ttccattcat aaatgggatt
     6601 cagcaggtag tgccttttat gcaaaaaaac atacccttcc tttctacata ttttctcaaa
     6661 ccgctcaata ctgatacccg tatccctgat ttccattagt gcctctaccg gttctttttt
     6721 ctttttcaaa atccacctgt aaatactgcg gggcagcaaa tggatatatg gcagtttaga
     6781 caaccttgaa cgacacattt gctgatggcc gccaaatggc atataccagg gaggaaatcc
     6841 aaagaacaca ataccaccgg ggttcagaaa atttttcatc cacccgatca atttcggctg
     6901 gtcgtgaata tgctcaatga catccttcag tacaataatg tcaaacctgc cgctaaaatc
     6961 tttttcgatg tccacgagat aaatatcttt tgctacaaac ctgatctttc cggcagccac
     7021 atcatccggt aaaaacttca atgcatcatc aattcttggt gtatccagtt ccactcccaa
     7081 accttcacag cctttattga cgaaagcttt caaaacaccc ccttcgccac aaccaatttc
     7141 taatacccgc atgccggttc tcaccggaaa tttttcttcg ataaaaggaa tgacatactt
     7201 ttcagcattc attacctgta tatcaaaata tctttttcta tctgcgtgaa attcaaacat
     7261 gccagttaag ctacttcttc taccttatcc atttcaatac gaaggttttc aataataaat
     7321 tcctgcctgt cgggtgtatt cttgcccatg taatattcga gtaattgctg aatgtggtct
     7381 ccttgttcca gtctcattaa ttgcttgcgc atatccgggc caatgaactg tgcaaattca
     7441 tccgggctta tttccccgag acctttaaac ctggtgactt caggtttacc gcccaatttc
     7501 ttcatagcgg tgagtttctc ggcttcatca taacaataaa tagtttcctg tttatttcgc
     7561 accctgaaca aaggcgtttc cagcacatag accttttcat gtttcaccag gtcagggaaa
     7621 aactgcagga agaaggtcat aagcaagaga cggatgtgca taccatccac atccgcatcg
     7681 gtggcgatca ctacattatt gtaacgcagg ccttccagcc cctcttcgat attcaaagca
     7741 tgctgcagca gattaaattc ttcattttcg taaacaacct ttttagtcag accaaaacag
     7801 ttaagcggtt tacctctcag gctaaacacc gcctgtgttt ctacatttct tgctttggtg
     7861 atggaaccgc tggcactatc tccttccgta ataaaaatgg tcgtttcttt ttgctttgcc
     7921 acaaattctt cttttccttt agccggcgct tcctcatcca aatgataacg gcagtcacga
     7981 agcttacggt tatgcaggtt ggctttctta gccctttcgt tagccagttt cttgattcct
     8041 gccagttctt ttctttctct ttcactttgt tcaattcgtt tcttcagagc ttcagcagtt
     8101 gccggatttt tatgcaggaa attatccagt tcctttgcca ggaaatctcc aacgaattgc
     8161 ttcatagaag ggccatcgat atctacattc actgagccca gtttagtctt cgtctgtgat
     8221 tcaaatactg gttctactac ccttacagaa accgcagcaa caataccggt tctaatatct
     8281 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caagcagccc gtttttactg
     8521 acaaacgttt tcccattgaa caatatcctt aagccggcat tcagaaaaca ataattccat
     8581 atctggtttt ccaggaattc gggcaggaat ttaaaatttt tgaaaaccga ttcatccggg
     8641 ataaaggtca ccatggttcc gttcagttca ccggtcttag cctccttatg ttcttttacc
     8701 agaacacctc tttcaaattc tgctgttttt tcctttccgt cacgaatggc ggttacttta
     8761 aagtaattac ttaacgcatt gacggccttg gtacccaccc cgttcaaccc cacagatttc
     8821 tgaaaagcct tgctgtcata ttttgctcca gtattaatct tactaactac atccaccact
     8881 tttcccaacg gtataccacg gccataatcc cttacggtaa ccgtttttcc ttcaatgctc
     8941 agctcaaccg ttttaccata tcccatggta tattcgtcga tgcagttatc catcacttcc
     9001 tttaccagta cgtagatgcc atcatccggg gacgacccat cgcccagttt gccgatgtac
     9061 attcccggac gaagccggat atgctccttc cagtcaagcg acttgatgct atcctcggta
     9121 taatcaaaaa gattggttgt gtctttctct tttgccatta tcacggtacg ttctaaagtg
     9181 taaacaaatg tttttcctgt agttatcggt tacggccaaa aactggttta gccattcggg
     9241 caaatatacc aaaacagcct taccgttaag gattgagagt tattaacaag tggggaaaaa
     9301 acgattatga aaactattcc tttttacggc cttttttctc cttctttttt ctggaattga
     9361 cagttgccag atcacccgca actacccatt cataatcaag ctgacgcttg gttaagttgg
     9421 cagccaccac ctttatccaa actttatcgc ccattctgaa tttacgaccg cttcgcagac
     9481 cggccaggca gtaatcagac tcaatatggc gaaactcgtc gtactccagt aaactattga
     9541 tactaacgag cccttcgcat ttgtgttcca ctgtctctac ccaaaacccg aaactggcaa
     9601 caccactgat cacaccttca aattcttcac ccaggaagtt cttcatgtac tgcacctgct
     9661 tgtatttatt ggccgccctt tctgcttcca ttgctgccct ttctctttca ctggtgtgtt
     9721 tacatttttc ttcaagcttc ttatccggat tcactttatc atttagcaca tcaaataaaa
     9781 ttctgtgtac ctgtatatcc gggtagcggc gtatgggtga agtgaaatga cagtaatgct
     9841 caaatcccag tccataatga ccaacgtttt ctgttgtata ctttgcttta gccattgtgc
     9901 ggatgcctag ttgttccagt acatgctgtt caggccggcc gtatacatcc tttagtaact
     9961 ggttaaatga agctgcaatg gtttccggtg aagaagtatc aaacttatgg ccgaactttt
    10021 tggcaaaagc ggcaaaaggc accaactttt cttcatccgg gtcatcatgt gtacggtaag
    10081 gaaatggtaa cggctattga ttttaatctt cgcaatattc tcagcaacag tgcggttggc
    10141 cagcaacata tattcttcga tcagctgatg tgcctcgaaa ctttccttaa tagtgatacc
    10201 aatgggatct cctttttcat tcagcttgaa gcgaacctcc tgtgaagaaa aattaatagc
    10261 accgttatca aacctttttt tccggaattt ctgtgcaata tcattcagta taaggatttc
    10321 ttcagcatat aacccggcct tctcttcaat gattgcttgt acttcttcat atgtaaaccg
    10381 atgatcggag tggataactg tttttcccaa ccagtactgc ttcacttcag ctttggcatt
    10441 aacctggaat atggctgaaa aggtcaattt atcttctttt ggcctcaatg aacacaatac
    10501 attggaaata tgttcgggca gcataggatt taccctgtat ggcaggtata cagaggtaga
    10561 acgatgataa gcttctttat ccagtgcatt atcaggttcc acatagtgac taacgtcagc
    10621 aatatgaata ccaatttcaa aatagccatt ctttaatttt cggaatgaaa tggcatcatc
    10681 aaaatccttt gcatctacag gatcaatagt gatcgtaaaa atatcccgta catcttttct
    10741 gcgcctgatc tcatcgccgg ggataacatc gggaatacgg gctgccactt caagtgcctc
    10801 ttccggaaac tccaacggaa atcctgcatc cagcagtatt tctttcatgg ctgcatcatt
    10861 actgttttca gggtccagca cggttactac ttcacccata ggccgcttac catcgctttc
    10921 ccattgtaat aaacgaacaa ctaccctgtc gttgtcatta gccccattaa tgttttcaag
    10981 cggaataaaa atatccggta tgggcttatc catttctgct acaaaaaagg caaatccttt
    11041 attcatttgc agatgaccta taaattcggt ccgctttctt tgaatcacct tttttatttc
    11101 gccctgcatt cttcttccac cgctttttat ttccttcact gctacctgca cggtatcacc
    11161 atgcaaggcc gtattaaagt cagcaggcct gatcataata tcggcgtcca ggccttccac
    11221 cgtcacaaat cccattcctg accgggttat atccattata cctttcagca attgtgctga
    11281 ctttcgctgt ctgtcttttt gtttcttctt tgaatttttt cttcccatga taaggttcaa
    11341 ttcttatttt ttaaagtctt taataaaatc cctgacgagc ctgtcaaaga ttattccctc
    11401 gttaaaaagg aactgatgcc tgaccgggat gacaaaaaat ggcaatcctc cgatctcatt
    11461 gctgaatttc tgcaatctga cagcatcttt ctctgttgtc agaattattt tttcttctga
    11521 ttccaattca tcaaaccgct tccggatctc attcaggtca tctatggtaa aaatatgatg
    11581 atctgaatac tgcaacagat gataaatatt actgtgctct tccagcaact tt
//
LOCUS       DAORLS010000066        11599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2964_length_11599_cov_1.880198, whole genome shotgun sequence.
ACCESSION   DAORLS010000066 DAORLS010000000
VERSION     DAORLS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_11599_cov_1.880198"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PK977_06860"
     CDS             <1..957
                     /locus_tag="PK977_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF17868.1"
                     /translation="FFALFPGMLNNLGKAGGWLNAVKVTLGFLELALALKFLSNADLV
                     QGWRLLDREIFIALWIVIFAMLGIYLLGKIKFHHDDELPKNDFGHPYLSVTRLFFAMA
                     SFTFVVYMLPGMWGAPLKGISAFLPPYGTQDFTPASGSSASTAHYVSGANPPTKYVKE
                     LGPYEPEVVKKYGLVTYFDYDEALAAARQLKKPLMLDFTGINCINCRKMEAQVWSSPE
                     VMRRLKEDFVVVSLYTDVRIKLPESEEFNSKELGEHVNTVGEKFMHLQASRFGAVSQP
                     FYIFLDGKEEKLAPDGYGYDPDVQAFIKHLDNVIAEYKKRTN"
     gene            complement(1110..>1465)
                     /locus_tag="PK977_06865"
     CDS             complement(1110..>1465)
                     /locus_tag="PK977_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF17869.1"
                     /translation="PWAMRIAHNLCVDHFRKVKRTPTIRNGEDKDIFEVLNFSEDSAE
                     TVMMKRQSHDRVRDMLDRLPEDQREVIILRHYADMSFKEIAAATNCSINTALGRMRYG
                     LINLRKLMTQKQIAL"
     assembly_gap    1466..1576
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1812..1845
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1992..>4328
                     /gene="uvrA"
                     /locus_tag="PK977_06870"
     CDS             1992..>4328
                     /gene="uvrA"
                     /locus_tag="PK977_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF17870.1"
                     /translation="MEKTIKGKIPANTKTVNSAENILIKGARVHNLKDVTVEFPRNKF
                     IVVTGVSGSGKSSLTIDTLYAEGQRRYAESLSAYARQFLNRMNKPDVDYIKGLCPAIA
                     IEQKVITRTPRSTVGSMTEIYEYLRLLFARIGKTISPVSGREVKKDDVTDVLNAITKL
                     KEGDKVYILATFKQHRNRDIKEELNILIQKGFSRVYSRESAGGSEESGSIYRIEELLE
                     VPDKELKKKKITHILVDRIVVKEMDEDDKHRVADSIGTSFYEGEGDVYLEVNGKTHLH
                     FNNRFELDGMQFEEPQPNLFSFNNPFGACPTCEGFSQVLGIDPDLVIPDRRLSVYEGA
                     VAPWKGEKLDWWRQNFIKRSKAVNFPIHKPVADLTDKQYKQLWEGVDGIGINDFLKDV
                     ESQLYKVQYRVLLSRYRGRTECPDCNGYRLRKEALYVKVADKHIGELSTIPAKELKAW
                     FDELDNKLSAHDKQVAKRILIEIHHRLNTLLSVGLGYLTMSRLANSLSGGESQRIQLT
                     RSLGSNLTNSLYILDEPSIGLHSRDTANLIKVLKELRGLGNTVVVVEHDEMMMREADH
                     IIDMGPMASHLGGEVVAEGDYDAIISNPNSLTGKYLKGELSIDPPKTVRKWNRSITVE
                     EARQNNLKNITVEFPLNVLCIVSGVSGSGKTTLVKQILYPALRKLKGEYGDKVGFHKQ
                     ITGDVDSIAQIELVDQNPIGKSSRSNPVTYIKAYDEIRDLYARQPLSKMRGFQPKHFS
                     FNVDGGRCDTCKGEGEQVVEMQFLADVHLVCESCGGKRF"
     assembly_gap    4329..4338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4339..>4724
                     /locus_tag="PK977_06875"
     CDS             <4339..>4724
                     /locus_tag="PK977_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRF17871.1"
                     /translation="VLEIKYKDKNIFDVLEMSVDEAINFFYDEKGLANALQPLSDVGL
                     GYVKLGQSSDTLSGGEAQRVKLASFLGKGRTGNKILFIFDEPTTGLHFNDIKKLLASF
                     NALIEQGHSIIVIEHNTDVIRSADWV"
     assembly_gap    4725..4904
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5046..>5393
                     /locus_tag="PK977_06880"
     CDS             5046..>5393
                     /locus_tag="PK977_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17872.1"
                     /translation="MKGLITAIFCCIIYNTASAQNTYTWNVAGGGNWNTSGNWTPART
                     STAASDILIINNGGTKTITNVPTQTIGRLVIANNTSVTLSGGGGSQTLTIANGTGNDF
                     IIESGSTLIQNNIL"
     assembly_gap    5394..5404
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5405..7801
                     /locus_tag="PK977_06885"
     CDS             <5405..7801
                     /locus_tag="PK977_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17873.1"
                     /translation="ENITLSSGCSADISGIYEVRGTFTTTASGAVTTVWGELVNTSMI
                     NSTTADKLVLKNGAVYRHNRNGGNVPTATWETGSDLLISGVTNSLPGNLSQSFDDFEW
                     NNPGQTTGFNLGGAITSVAGDFKVTNTNNYGLFLTNTSGSTYSLSVSGDLFLSGNSWL
                     SLTNGDNITATIDIAGNIIMSGGYFDYHVATSGGTTLNKIVVNVSGDVLITGGIYDFA
                     WGNSNAANFTELRLGGNLSVSGTGIFRTTTTDADISNGTVYFNGPGNLQTFYAETPAN
                     ITYVNFTVNNRLRLLSDRILASNNVAGWADQFIINSGGSIDLSTYRIISSTGSSAAAN
                     SSIVLQAGAEIITANADGLQLNETNGSVSTAIANRTYSSGANYVYNGSVVQNSGIFTT
                     SPVVNQVNNLTINNTSGTYSTGLTLQQPIAVNGNCFFTAGVITTSLTDILIINDGATA
                     TGANNNSSNPSFVNGPVRKVGNDAFEFPVGKINAGYRPCSISAPALTTDVYTAEYMRD
                     NATALGAITAPGLQAVSNCEYWDIDRTAGTSSVNVTLSWSSISPCNTGAAVSDLDDIT
                     VAHFDGNNWNSHGSSAINGMATSGTVTWNGVNNFSTFSLGIKSSQSPLSVKFTNLTAY
                     PSGSANIIEWTINEESGVQDYEVQRSSNGANFITINTTLARNNSGSNEKYRFREEQPG
                     TDKLFYRIKANELDGTITYSPVAKVIRSGSNEDAIKLYPNPVTGKQFSLQLNKLEKGN
                     YVLRLINSKGQEVHQFSLQHAGGYLYRSVELPSNIPVGIYYLLMTGNEVMFRSALIVQ
                     "
     gene            complement(7847..8263)
                     /locus_tag="PK977_06890"
     CDS             complement(7847..8263)
                     /locus_tag="PK977_06890"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRF17874.1"
                     /translation="MIQRRQTLWLLLATIAAVLSFMFPFVTGKEAIKNMQADKVIDAA
                     SDFFLLILTGGSIILSTVTIFLFKDRKMQIRLCLLGLLLSIVIIVRYIMLMNKLTNTT
                     LALYAILPFVFLASYFFAFRDIRKDEKLVKSLDKLR"
     gene            complement(8299..9576)
                     /gene="glyA"
                     /locus_tag="PK977_06895"
     CDS             complement(8299..9576)
                     /gene="glyA"
                     /locus_tag="PK977_06895"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRF17875.1"
                     /translation="MQKDTLVFDLIRKELDRQRSGIELIASENFTSLQVMQAMGTVPT
                     NKYAEGYPGKRYYGGCEVVDEIETLAIERLKKVFNCSWANVQPHSGASANAAVFLAVM
                     KPGEKFLGLDLSMGGHLTHGSPANFSGKTYHAIHYGVTKDGIIDYEQLEAKAREEKPK
                     MIICGASAYSRDWDYKRIRAVADEIGAVVFADIAHPAGLIATGLLNDPFEHCHIVSST
                     THKTLRGPRGGVIMLRNDFENPWGIKDPKGNLRTMSQLLDLAVFPGSQGGPLEHVIAA
                     KAVAFGEILSEDFKSYGKQVIANAQAMAKSFVNRGYQLISGGTDNHLMLIDLRNKNLT
                     GKKAQETLDKAHITLNKNAVPFDDKSPFVTSGIRVGVPAITTRGLKEDHMETVVSYID
                     TVLMNTDDEATINKTKDEVKAFMQQFPLYPELG"
     gene            9715..10704
                     /locus_tag="PK977_06900"
     CDS             9715..10704
                     /locus_tag="PK977_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF17876.1"
                     /translation="MKAIIPVAGAGTKLRPHSYTQPKALIPLAGKTVLSIIIDQLKSA
                     GINEFVFIVGYLGDKIQDYVKAKYPDIKAHYVHQYDRQGVGHAIKLARTIINQDEVFV
                     VLGDTICEYDVQEVVNSPHSMIGVRKVDDPRDFGVAEIDEDCSISHVVEKPQIPKSNM
                     AMVGLYKIRESDQMFQCLENNIRQGLRSHGEYSLTDALDCMIKMGVQFKSFKVENWFD
                     CGRKETLLESNATLLKKFAPVTDARLYENTVVIPPVSIGAGCTIKNSIIGPNVTIGEN
                     TVIDDSIVKNSIIGSFSNLFDIVLDESVIGSDTNLRGETRSLNIGDNTSIDLG"
     gene            10833..>10904
                     /locus_tag="PK977_06905"
     CDS             10833..>10904
                     /locus_tag="PK977_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17877.1"
                     /translation="MIWASGWRLASAVSNAGGLGIIGA"
     assembly_gap    10905..10938
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10939..>11599
                     /locus_tag="PK977_06910"
     CDS             <10939..>11599
                     /locus_tag="PK977_06910"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRF17878.1"
                     /translation="EHLQKCKAAIDKPFAVNLPLLYPDIDKHINILIEEDVKIVFTSA
                     GNPKTWTGMLKEKGITVVHVVSSSKFARKAEEAGCDAVVAEGFEAGGHNGREETTTMV
                     LIPAVVNAVNIPVIAAGGIATGRQMLAAMVLGAEGVQMGSRFVASEEASSHNSFKQAV
                     IRSSEGDTQLMMKQLTPVRLLKNAFFTEVQQAELRGATVEELKTLLGRARAKKGMFEG
                     DI"
BASE COUNT         3323 a         2155 c         2576 g         3165 t
ORIGIN      
        1 ttctttgcct tattcccggg aatgcttaat aacctgggta aagcaggtgg ctggctgaat
       61 gctgtgaagg tgacacttgg ctttttggaa ctggcacttg cccttaagtt tttatctaac
      121 gcagatctgg ttcagggttg gcgactactt gaccgggaga tatttatagc tctttggatt
      181 gtcatattcg ctatgttggg tatttacctc ttaggcaaga tcaaatttca tcatgatgat
      241 gaattaccga aaaatgattt tgggcacccc tatttaagtg ttacccgttt gtttttcgct
      301 atggcttctt tcacatttgt ggtttatatg ttgccgggta tgtggggtgc acctttaaaa
      361 ggtatcagtg cctttttacc tccgtatgga acgcaggatt ttactcctgc atcaggatcc
      421 tctgcttcaa ctgctcatta cgtttcgggt gccaacccgc caacaaaata tgtaaaagag
      481 ctaggtccat atgaaccaga ggttgttaaa aaatatggcc ttgttactta ctttgattat
      541 gatgaagccc tggcagctgc ccgccagtta aagaagccat tgatgctcga ttttacagga
      601 atcaactgca ttaattgccg gaaaatggag gcgcaagtat ggagcagccc ggaagtaatg
      661 agaaggctga aagaggattt tgttgttgtt tcgttgtata ccgatgtaag gatcaaatta
      721 cccgagagcg aagaatttaa ttcaaaggag ttaggtgaac atgtgaatac ggtgggagaa
      781 aaatttatgc acctgcaggc aagcaggttt ggtgcggttt cgcagccatt ttatattttc
      841 ctggatggta aagaagaaaa actggcaccg gatggttacg gttatgatcc ggatgtgcag
      901 gcttttatca agcacctgga taatgtaatt gcagagtaca agaaaagaac taactaacga
      961 agtggtataa taaagaaaag accccgtcat ttgacggggt cttttcaatt tggtgtcatt
     1021 ctgtaccagc ctcccggtat ggcaagcaaa caacattcct tgattcagaa attgtttttc
     1081 tccgggtgga gatacagaat gacaaaaaat taaagagcaa tttgtttttg ggtcatcagc
     1141 ttgcgcaaat taattaatcc gtagcgcata cggcctaaag cagtgttaat actgcaattg
     1201 gtggctgctg caatctcttt aaaactcata tctgcgtaat gacggagaat gatcacttcc
     1261 cgctggtctt ccggcagcct gtccagcata tcccttaccc ggtcatgact ttgtcttttc
     1321 atcattaccg tttcagcgct gtcttctgag aaattcagca cttcaaagat gtctttatct
     1381 tctccgttac ggatggtggg ggttcttttg actttgcgga aatggtcaac acataaattg
     1441 tgtgctatac gcatggccca tggaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnttga ggtatacaac ttgtccttgt gacgcagcac aagtgcttca
     1621 agggcatcca cattgccatc tgtaaaaaga tggatcagtt catagtcggt cttttttgct
     1681 aaagctttca taactcctac aggtttaggt tgctttccgt tattgcatag caataacaga
     1741 ggttcattgg atattggtta caataaaatc ggttaaaaaa acagagtaga agttatgcga
     1801 taggctgggt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatatt gtattggact
     1861 ttaaaaatag gccatttcat gaaattgcca aacatttgtg aaatcaaatt gtagttttgg
     1921 gtgctaagaa aatgttaaga ctctgcccgg aatacaaaga ttcataaggg attacatttg
     1981 cagactatta aatggaaaag acaataaaag ggaaaattcc cgctaataca aagaccgtaa
     2041 attccgcaga aaatatcctt ataaaaggtg caagggttca caacctgaaa gatgttactg
     2101 tagaatttcc acgaaacaag ttcattgttg taaccggtgt atccgggtcc ggcaagtctt
     2161 cccttacaat tgatacatta tatgcagaag gtcaacgtcg atatgcagag agcttaagtg
     2221 cttatgcacg gcaatttctt aaccggatga ataagcctga tgtggattac ataaaaggcc
     2281 tatgccctgc tattgccatt gaacagaaag taataacccg tacaccccgc agtactgtag
     2341 gcagcatgac cgagatttat gaatatttac gtttattatt tgccaggatt ggaaaaacca
     2401 tttcacctgt ttcgggaaga gaggtgaaaa aagatgatgt gacggatgtt ttgaatgcca
     2461 ttacaaaatt gaaggaaggc gataaggtgt acatacttgc aacatttaag caacacagga
     2521 acagggatat caaagaagaa ttgaatattc tcatacaaaa aggattctcc agggtttata
     2581 gtcgtgagtc tgcaggtggt agtgaagaat caggaagcat atacaggatt gaagagttgc
     2641 tagaagttcc agataaggaa ttaaaaaaga aaaagattac acatatcctg gtcgacagaa
     2701 ttgtggtaaa ggaaatggat gaagatgata aacaccgggt agcagattcg attggtactt
     2761 cattttatga aggagagggc gatgtatacc tggaggtaaa cggtaaaacc catttgcatt
     2821 ttaataaccg ctttgagctg gacggaatgc agtttgaaga gccgcagccg aatctattct
     2881 cattcaataa tccatttgga gcctgcccta cctgcgaggg tttttcgcag gtattgggta
     2941 ttgatccgga ccttgtaata cccgacagaa gattgagtgt atatgagggc gccgttgcac
     3001 cctggaaagg agagaagtta gactggtgga gacagaattt tataaaacgg tcaaaggctg
     3061 tcaatttccc cattcataaa cccgttgccg atcttaccga taaacaatat aagcagcttt
     3121 gggaaggggt agatggaata ggtattaatg atttcttaaa agatgtagaa tctcagctat
     3181 acaaagttca atacagggtg ctcttaagcc ggtacagggg cagaacggaa tgcccggatt
     3241 gcaatggcta tcgccttcgt aaggaagctt tgtatgttaa agtagctgat aagcatattg
     3301 gagagttaag taccatacct gcaaaagaac ttaaggcctg gtttgatgaa ctggataata
     3361 agctcagtgc acatgataag caggtggcaa aaagaatact gattgaaatt caccatcgcc
     3421 tgaatacttt gttgagtgtt gggttaggtt atcttacaat gagccgcctg gccaattctt
     3481 taagtggggg tgaaagccag cgaattcagt tgactagaag tttgggcagt aacctgacaa
     3541 attcattgta catacttgat gaaccgtcca tcggcttaca ttcgagggat acggctaacc
     3601 tgatcaaagt attgaaagag ttaaggggcc ttggtaatac cgtagtggtg gtagagcatg
     3661 acgaaatgat gatgcgggaa gctgatcaca tcattgacat gggacctatg gcatcacacc
     3721 tgggtggtga ggtggtggcg gagggagatt atgatgccat tatatcaaac cccaacagtc
     3781 ttacgggaaa atacctcaag ggagagctta gtattgatcc gcccaagact gttcgcaagt
     3841 ggaacaggtc aataacagtt gaagaagcaa gacaaaataa cctgaagaac attactgttg
     3901 agttcccgct taatgttctt tgcatagtta gtggcgtaag cggtagtggt aaaacaacgc
     3961 tggtaaaaca aattttatat cccgccttgc gtaagcttaa gggggagtat ggtgataaag
     4021 taggttttca taaacaaata accggtgatg tggacagcat cgcacaaata gaactggttg
     4081 atcaaaaccc gataggtaaa tcatccagaa gcaaccccgt tacttacatc aaagcatatg
     4141 atgagatcag ggatttgtat gcaaggcaac cattgagcaa gatgagaggt tttcagccca
     4201 aacatttttc atttaatgtt gatggtggtc gttgtgatac ctgcaaggga gaaggagagc
     4261 aggtggttga aatgcagttt ctcgcagatg tgcacctggt ttgtgaaagc tgcgggggta
     4321 agcgatttnn nnnnnnnngt actggaaata aaatacaagg acaaaaacat ttttgatgtg
     4381 ctggaaatga gtgtggatga agcaatcaat ttcttttatg atgaaaaggg acttgcgaat
     4441 gctttacaac ctttaagtga tgtggggctt ggctatgtaa aactgggcca gtcatcagat
     4501 actctttcgg gtggagaggc acaacgggtg aaactggctt cattcctcgg aaaaggaaga
     4561 accggcaata agatactttt catttttgac gaacccacca ctggtcttca cttcaacgac
     4621 attaaaaaac tattggcttc tttcaatgcc ctgatagagc agggacattc cattatagta
     4681 atcgaacaca atacagatgt cattcgaagt gccgattggg tgatnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntacgat gtcggaaaag
     4921 gtgtatttac tttttttgat tcttctttta ggtaaaaacc attatttctc ctaagtagat
     4981 atataattta gcccttagaa ttcgttagtt aaaaaccctt agtggttcat ccaaatatcc
     5041 tgacaatgaa aggtctgatc acagcaatct tttgctgtat tatatataat acagcttcag
     5101 cgcaaaacac ttacacatgg aatgtggcag ggggtggcaa ctggaatact tccggtaact
     5161 ggactccggc cagaaccagt acagctgctt cagatattct gataatcaac aatggcggca
     5221 ccaaaactat tacaaatgtg cctacacaaa caatcggcag attagtgatt gccaacaata
     5281 cttctgtaac cctatcaggc ggcggcggat cacaaacact aaccattgcc aatggaaccg
     5341 gaaatgattt tattattgaa agcggatcaa ctttaattca gaacaatatt ttannnnnnn
     5401 nnnngagaac attacccttt catctggttg cagcgctgat ataagcggta tctatgaagt
     5461 gaggggaact tttaccacta cagcgtctgg tgcggtaact actgtttggg gagaactggt
     5521 aaatacttca atgataaatt ccaccacagc ggataaactg gttttaaaaa atggcgccgt
     5581 gtatcgtcat aacaggaatg gaggcaatgt tcccacagca acttgggaaa ccggctctga
     5641 tttattaatt tctggtgtta caaattcttt gcccggaaac ctcagccagt cttttgatga
     5701 ttttgagtgg aataacccgg gacaaaccac cggatttaat cttggcggag ctattacatc
     5761 tgttgcaggt gattttaagg tgaccaatac caataactac ggtttgtttt tgaccaatac
     5821 ctccggaagc acttatagtt tatcggtttc aggagatctt tttttatctg gtaattcttg
     5881 gctttcttta accaatggcg ataatatcac agctactatt gatatcgcag gcaatattat
     5941 tatgtccggc ggttattttg attatcatgt agccacatcg ggaggtacaa cacttaataa
     6001 aatagttgta aatgtaagcg gagatgtatt gataactggg ggtatatatg actttgcctg
     6061 gggtaattcc aatgctgcta atttcacaga attgcgcctg ggaggaaatc tttctgtgtc
     6121 tggcaccggc attttcagaa caactacaac tgatgcagat atttccaacg gcacagttta
     6181 cttcaatggc ccgggtaacc tgcaaacttt ttatgcagaa acaccggcaa atattactta
     6241 tgtaaatttt actgtgaata acagacttcg tttattatca gacaggattc ttgccagtaa
     6301 taatgtggct ggatgggcag atcagtttat tattaatagc ggcggcagta tcgatctgag
     6361 tacataccga ataataagtt caacgggttc ttctgcagcg gcaaacagtt ccattgtgtt
     6421 acaagcaggt gcagaaatta ttaccgccaa tgctgatggt ctacagttaa atgaaacgaa
     6481 tggctctgtt tctacagcca ttgccaacag aacatatagc agcggggcca attatgttta
     6541 taatggttcg gtagtacaaa acagcgggat attcactact agtccagtgg tcaaccaggt
     6601 taataatctt accattaata atacttccgg aacgtatagt acgggactta cactgcagca
     6661 accaatagct gtgaatggga attgcttttt tacggcaggt gttattacaa cttctttgac
     6721 ggatattcta atcattaatg atggtgcgac tgccactggt gccaataaca attcatcaaa
     6781 cccttctttt gtgaacggac ctgttcgcaa agtgggtaac gatgcgtttg agtttcctgt
     6841 tggaaagata aatgcgggtt accggccctg cagcatatcg gccccggcac ttacaacaga
     6901 tgtatatacg gcagagtata tgagggataa tgccactgcg cttggcgcaa taacagctcc
     6961 gggtttacag gcggtcagta actgtgaata ctgggatatt gacagaacag cgggtacatc
     7021 ttctgtaaat gttacattat cgtggagcag catcagcccc tgtaatacag gtgcagctgt
     7081 aagcgattta gatgatataa cagttgcaca ttttgacggc aataactgga actcccacgg
     7141 atcaagtgcc ataaacggga tggccacctc aggtacggtt acctggaatg gcgttaataa
     7201 tttcagcact ttttccctgg gtattaaaag ctcacaatcg ccactatctg taaagtttac
     7261 caatctcaca gcttatcctt cgggttctgc taatatcatt gaatggacga tcaatgagga
     7321 atccggtgta caggattacg aagtacaacg ttcatcgaat ggtgcaaact ttattacaat
     7381 aaacactaca cttgcccgga ataatagtgg aagcaatgag aaatacaggt tcagggagga
     7441 acaaccaggt acggataaat tattttaccg gatcaaagca aatgaactgg atggtactat
     7501 cacctacagc ccggttgcca aagtgataag aagtggtagt aacgaagatg ccataaaact
     7561 ttatcccaat cctgttaccg gaaaacagtt tagccttcag ctcaataaac tggagaaagg
     7621 aaattatgta ctccggctga tcaacagtaa aggacaggaa gtgcatcagt tttctctgca
     7681 gcatgcaggc gggtatttat accgttcggt tgagttaccg tcaaatattc ctgtgggaat
     7741 atattacctg cttatgactg gaaatgaagt tatgtttaga tctgccttaa ttgttcaata
     7801 gcatttcggg gcatttcgaa agctagcttt tgattcttga agttattcat cgcaacttat
     7861 caaggctctt caccagtttc tcgtcttttc gtatatccct gaaagcaaaa aaatagctgg
     7921 caagaaaaac gaaaggcagt atagcgtaca aggcaagtgt agtattggtc agtttattca
     7981 tcagcatgat ataacggact ataattacaa ttgaaagcaa taaacccagc aggcaaagcc
     8041 gaatctgcat tttcctgtcc ttaaaaagaa aaatagttac ggtagatagt attattgacc
     8101 cgccggtaag tatcagcagg aaaaaatcgc tggcagcatc aatcacctta tccgcctgca
     8161 tattcttaat tgcttcttta ccggttacaa aagggaacat gaaactgagc accgctgcaa
     8221 tagtggcaag caataaccaa agtgtttgtc gtctctgaat catttaactg attttattct
     8281 caaagaatat ttttataatt aaccaagttc aggatacagc gggaactgct gcatgaaagc
     8341 cttcacttca tcttttgttt tgttgatagt tgcttcatca tcagtattca ttaatactgt
     8401 gtcaatataa gatacaacag tttccatatg atcctctttt aatcccctgg tggtaatagc
     8461 tggaacgcca acccttatgc ccgatgttac aaaaggagat ttatcatcaa aaggcactgc
     8521 atttttattt agtgtaatgt gggctttgtc cagcgtttcc tgtgcttttt tgccggtcag
     8581 gtttttgttc cgaagatcaa tcagcatcag gtggttgtcc gtaccaccac tgattagctg
     8641 gtaaccccgg tttacaaatg atttggccat agcctgtgca ttggcaatta cttgtttgcc
     8701 gtatgattta aaatcttcag atagaatttc accaaaggct acagctttgg ctgcaattac
     8761 atgctccagt ggtccgccct gtgagccggg aaacacggcg aggtcaagca actggctcat
     8821 ggtccttaaa ttgccttttg gatcttttat tccccatggg ttttcaaaat cgttgcgtag
     8881 cataataaca cctcctctcg gtccacgcag tgttttatgg gttgtagaag aaacgatatg
     8941 gcaatgttca aaaggatcat tcaataaacc tgttgctatc aggccggccg ggtgtgcaat
     9001 atcagcaaat actacagctc ctatttcatc ggctacggca cggattcttt tataatccca
     9061 gtcacggctg taagcagaag cgccacaaat gatcatcttt ggtttttctt ctcttgcttt
     9121 ggcctccagt tgttcataat cgataatacc atctttcgtt actccataat gaatagcatg
     9181 gtaagttttg ccactaaagt tggcgggact accatgggtc aggtggccgc ccatactcag
     9241 gtcaaggcct aggaattttt cacccggttt catcactgcc aggaagacag cagcattagc
     9301 ggaagcaccg ctatgcggct gcacattggc ccagctgcag ttgaaaactt tttttagtct
     9361 ttctatggct aatgtttcta tctcatcaac tacttcgcaa ccgccatagt atcttttgcc
     9421 gggataacct tctgcatatt tattggtagg aacggttccc attgcctgca ttacctgcag
     9481 ggaagtgaaa ttttcggaag cgataagttc tataccgctg cgctgacggt ccagctcttt
     9541 gcgaatgagg tcgaaaacca aagtatcttt ttgcataagg caaaaataag agagtaacaa
     9601 tggaatagcc ccaaactgtt caggattttg ttttattcta aaagtattac tttcgttggc
     9661 ttttgattag ttaccaccgg cagtaattgc cggacaaata cagtggctta acacatgaaa
     9721 gctatcatac cagtagccgg tgcaggcacc aagctgcgtc cacatagtta cactcagccc
     9781 aaggctttga ttccattggc gggtaaaaca gttctaagta tcatcattga ccagttaaaa
     9841 agtgcaggaa tcaatgagtt tgtttttatt gttggttacc tgggcgataa gatacaagac
     9901 tacgtaaagg caaaataccc ggatataaag gcacattatg ttcaccagta cgacaggcag
     9961 ggagtaggac atgccattaa gctggccagg accattatta accaggatga agtatttgta
    10021 gtgttaggag ataccatttg tgaatatgat gtacaggaag tagtgaacag tccacattcc
    10081 atgatcggtg taagaaaagt ggatgaccca agagattttg gtgtggcaga aattgacgaa
    10141 gactgctcaa tcagtcatgt ggtagaaaag ccacagatac ctaaatccaa tatggcgatg
    10201 gttggcttgt acaagatcag ggaaagcgac cagatgtttc aatgcctgga gaataatatc
    10261 cgccaggggc ttcggagtca tggtgagtac agcctgacag atgctttaga ctgtatgatc
    10321 aaaatgggag tgcagttcaa gtcatttaag gtcgagaatt ggtttgattg tggtcgtaag
    10381 gaaacattac tcgaatcaaa cgccacattg ttgaaaaaat ttgctcctgt aacagatgcc
    10441 cgtctgtatg aaaatactgt tgttatacct cctgtaagta ttggtgcagg gtgcaccatc
    10501 aaaaactcga ttatcgggcc aaatgttacg atcggtgaaa atactgtgat tgacgattct
    10561 attgttaaga actccattat tggttctttc tccaatctct ttgatattgt actggatgaa
    10621 tcagttatcg gcagcgacac caatctccgg ggagaaaccc gttcactgaa tattggtgat
    10681 aacacaagta ttgaccttgg atagaccagt cattagcctt gagtcaatag ttatctttac
    10741 tacatcaaaa caaatcctta ctgatgcagg ctcaattctc caaccgaatt acccaacttt
    10801 ttggcattga ataccccatt attcaggcag gtatgatctg ggccagcggc tggcgattgg
    10861 ccagtgctgt aagcaatgcc ggcggcctgg gaatcatcgg agctnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnga gcatttgcag aaatgcaagg ctgctattga taaaccattt
    10981 gccgtcaacc tgccattgct ttacccggac atagataaac acataaacat attaattgaa
    11041 gaggatgtaa aaattgtttt tacttcagct ggcaatccaa aaacatggac aggtatgctg
    11101 aaagaaaagg gtatcacggt agtgcatgtg gtcagttcat ccaagtttgc ccgtaaggca
    11161 gaagaggcag gctgtgatgc ggtggtggca gagggttttg aagcaggtgg gcataacggg
    11221 agagaggaaa caactactat ggtacttatc ccggcggtgg ttaatgcagt taatattcct
    11281 gtgattgctg ccgggggtat tgccacgggt cgtcaaatgc tggcagcaat ggtgcttggg
    11341 gctgaagggg tacaaatggg aagccggttt gttgccagtg aggaagcctc gtcacataac
    11401 agttttaagc aggctgtgat tcgttcttca gaaggcgata ctcaattgat gatgaaacaa
    11461 cttactcctg taaggctgct aaaaaatgcc ttttttaccg aggtacagca ggccgaactc
    11521 aggggggcaa ccgtggaaga gttaaagacg ttattaggaa gggcaagggc aaaaaaaggg
    11581 atgtttgaag gagatatag
//
LOCUS       DAORLS010000067        11541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2988_length_11541_cov_2.348424, whole genome shotgun sequence.
ACCESSION   DAORLS010000067 DAORLS010000000
VERSION     DAORLS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_11541_cov_2.348424"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="PK977_06915"
     CDS             <1..1611
                     /locus_tag="PK977_06915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRF17879.1"
                     /translation="RSLGTVAELEQWLKDASELEAVISEDACWRQIKMTCDTENKQLE
                     EAFNFFMMEIQPMIQPYADKLNKKLLACPHLSSLDQSKYFTYLRNVKKNIELFREENI
                     QLNAELNVMQQQYGVIAGKMTVQVNGQEYTLQQAAKFLEDPDRKLREEVYRKINERRL
                     QDKEELNTLFSNLLEKRHRVALNAGFANYRDFRFKELGRFDYTKDECYQFHEAVKLHV
                     MPLVNQIYEAKKKKLGLDTLRPWDVEAEPAGIQPLRPFKTGEELTEKTIACFRGLKTF
                     FADCLQKMKAMGHLDLESRKGKAPGGYNCPLAESGAPFIFMNAAGTLDDVTTMVHEGG
                     HAIHSFLSHNLELTGFKEYPTEIAEVASMAMELFSMDKWDVFFDNADELKRAKEQQLE
                     RVITIFPWIATIDKFQHWIYENPVHTEEDRAGKWMEILNEFSSPIVDVQGLDEYRRYG
                     WQRQLHLYEVPFYYIEYGIAQLGAIGLWQQYKQNPANAVNNYITALSLGGTRTLPELF
                     KAAGLDFNLSPGYIKGLMEFVKKEADSL"
     gene            complement(1775..2215)
                     /locus_tag="PK977_06920"
     CDS             complement(1775..2215)
                     /locus_tag="PK977_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRF17880.1"
                     /translation="MPKQSANEIAVRIESLLKRNRLSVTDSRRKILQLFLEHTGAMAH
                     GDIEKGAGEKFDRVTVYRTLQTFVDKGIIHIIPTADNSIRYALCKDDCGEGHHHDHHI
                     HFVCNHCNKTYCLDDVVTPDIKLPRGYSAGHVEVVVEGICKNCQ"
     gene            complement(2219..2827)
                     /locus_tag="PK977_06925"
     CDS             complement(2219..2827)
                     /locus_tag="PK977_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRF17881.1"
                     /translation="MSKKGVFYLSFFLLLAFGSYFVLAQVIPGFTKKGVAPISFVRPF
                     AFTNQDGKTITEKDIKGKVYVAGFFFTTCKSICPTMNNYLKTVYEEFKNEKDFLILSH
                     TCDPETDSVAQLKHYTDSLGVNTDKWIFLTGRKDSLYNMARVSYTIDDPANNLKSIED
                     QFIHSQFWALVDRNGDVRKIYDGLKEEEVKELIGDIKKYLQE"
     gene            2918..3310
                     /locus_tag="PK977_06930"
     CDS             2918..3310
                     /locus_tag="PK977_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HRF17882.1"
                     /translation="MKLKINWDALGIGATLACAIHCAMLPLLMSSLPLFGINIIHHTG
                     FEVGMILLALAIGTYSLWHGYRKHHHSILPIAIFYSGFAFLVLKQFFLHYSTWLLIPA
                     VLLIITAHLINYKSCRVHNHAHSDDCNH"
     gene            3391..3816
                     /locus_tag="PK977_06935"
     CDS             3391..3816
                     /locus_tag="PK977_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HRF17883.1"
                     /translation="MKKFSSFIVCLFFGISILAQSKQEKSILAILGKQTQAWNRGDIE
                     AFMNGYWENDSLMFIGKSGVTYGWKNTLNNYKKGYPDTAAMGKLTFTIIQVKKLTAKY
                     YHVTGKWLLKRSVGDAGGHYTLLFRKIRWRWVIISDHSS"
     assembly_gap    3825..3847
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3848..4058)
                     /locus_tag="PK977_06940"
     CDS             complement(<3848..4058)
                     /locus_tag="PK977_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17884.1"
                     /translation="MEQGWDPEVKKFFLRIINTVSWGLLWLMACATAGLYFQLAYKQG
                     IYTVIFYVLMAITFFLLLRYFFKIWK"
     gene            complement(4113..4886)
                     /locus_tag="PK977_06945"
     CDS             complement(4113..4886)
                     /locus_tag="PK977_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRF17885.1"
                     /translation="MLTRRQKYKLRRNLLLVMGAVVLITGSWLFWQWKTYRKARSVRY
                     PEFGIVIPGGYTIHGIDVSKYQSSIAWEEVKAMNVQDIQLGFAFIKATEGIGNTDPHF
                     KRNWKRSKDAGIFRGAYHFFIASKDGRLQAENFIDKVELEPGDLPPVLDVEQLNGTSP
                     EQLKKEVKKWLDIAENHYGVKPIIYTNVDFYNRNLGSDFDNYPLWVAHYYQPEQPRIS
                     RGWVFWQHSDEGRVNGIVSPVDFNVFNGDSSDFKNLLLR"
     gene            4916..>5163
                     /locus_tag="PK977_06950"
     CDS             4916..>5163
                     /locus_tag="PK977_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17886.1"
                     /translation="MSNLIELPKFEKGICIFRYPTMSYAIEQFYRLFQGALFFQGIFF
                     GILFFLTKRKDIMWYALYLLAAAANFFINASGTFLNID"
     assembly_gap    5164..5181
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5182..5393
                     /locus_tag="PK977_06955"
     CDS             <5182..5393
                     /locus_tag="PK977_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17887.1"
                     /translation="NLVGKEAANNAVKYSGASELVIRTSKSKGQLALTVSDNGKGFNP
                     EAGYAGNGINNMKKRAEETGGQLSI"
     gene            5510..7471
                     /locus_tag="PK977_06960"
     CDS             5510..7471
                     /locus_tag="PK977_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF17888.1"
                     /translation="MFSKQLKYLGLLLCLNCVIASAQYVAPDSLKKIIATAKKETDRI
                     DAMNWLAFRYYQKLDTSSCRIWAERALSSSVKIGYKKGEGFAYINLGNINYAYGPDSL
                     VTKYYQQAKKALETAGDKGLLGIVHSNLASHSMFKQSYLESLRELNKSLRLQQESGFQ
                     FGLAHSYMSIGTYHFLMGDYIEAEKNLSKSEHMFRVQNENTLLSYSYYFGGLTYSALK
                     KYRLAKAHLDMAREIFKNSGETVWFEIEYYRCVAMICLERADSCLTAKNKTLAVENFM
                     LSEKNLNIAVQKNTINDYDQFGLIYNNLGLLYLSWKKYALSKKFFLKSLESARITRDK
                     RIFDGSHDGLSQIDSINNDFESALANYKNHILYRDSINSEKNIRDIERYKIEFETEKN
                     LDDIKLLTAENELNTAIVSKQKQQKNFVFACTGILLLAGIYGFQRYRNKRRIQSQQEI
                     AEERQRISRDLHDEIGATLSGIAMYGHMVKNSLGNSNTDMAVQSADIIQESATEMVNK
                     LNDIVWLIKPQNESMEALIGRLNIYAAEMCAAKNIVPDIQVTGEAAGSAPPLEQRKNI
                     YLICKEAINNAAKYSGATVLTMRFFVEEKWLHISIKDNGQGYDPENSKNGNGLENMQN
                     RARQIGASLDILTAPGNGCAVELKLKITQ"
     gene            7512..8159
                     /locus_tag="PK977_06965"
     CDS             7512..8159
                     /locus_tag="PK977_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF17889.1"
                     /translation="MDIKVAIFEDGKLIREALQTILNGTPGYACTGAFSNGSNWELDI
                     RRTNPDVVLMDIEMPGLDGIELTRLISQKFEGIKVLIQTVFDDSQKIFNALCAGASGY
                     ILKNDPPNKYLEAIAEVYNGGSFMNASVAKKTLNFFTQKNIILTSPEGTDYGLSEREK
                     EILKELTEAQPLKNIAAKLYISLETVRTHVKHIYAKLHVANRTEAVMKAVQQGIA"
     assembly_gap    8374..8409
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8410..8792
                     /locus_tag="PK977_06970"
     CDS             <8410..8792
                     /locus_tag="PK977_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17890.1"
                     /translation="IPNSSLYIIPPAGFAVDELSGTLAQTNGTAHYMQMQILAGYTKE
                     KLFSEFKSEADKNFPGSWKEEAVSIAGHNATIYHNKTAAYSQYYMAFADGYTDEMIIA
                     NYEESEAATGKEMYEALKTVVVKK"
     gene            8804..>9082
                     /locus_tag="PK977_06975"
     CDS             8804..>9082
                     /locus_tag="PK977_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17891.1"
                     /translation="MQNSILKSVATVCLLFFIHFANAQIKNLHPQSHKIPDSLVMDKV
                     TGKASVRYVDEKYMKDGWNVFSHQPGRFIYVDYKNGVVRGFYTSDTSGK"
     assembly_gap    9083..9152
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9529..9567
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9833..10744
                     /locus_tag="PK977_06980"
     CDS             9833..10744
                     /locus_tag="PK977_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17892.1"
                     /translation="MTNLIYSLTLLLLLKQTEIVPIIKNAAQPDLIVKASHFRLDAAN
                     KRIYFKAEISNIGDAPCTIHPGPGGGYGIQTYLSADNSIGNDVVSGGRVFDELFTFTL
                     QPGESRLLDGEHSAGYSQLGSLSGYPYLVVELQSPNIESNRTNNKSIYKHGLPRKKGE
                     GLEAPRPDNIPTQLAPKAIDLKLLISDVTSQLVTEPETGKQYKEYTYTATIKNIGNIN
                     AVIDLEKPISLQYWKTTSCTDTSGMTSMQRPENQHAAFVLEPQKNFSLPLRKVRQKPD
                     AITPLLVQLVYDVSEKNKWNNFACLGN"
     gene            10858..11217
                     /locus_tag="PK977_06985"
     CDS             10858..11217
                     /locus_tag="PK977_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17893.1"
                     /translation="MKKIILMAFVICLAFFNAKAQTPATNINAKVETSRKANGDVLIH
                     TEEGKFKVYASFKKSKATGYYAVDMTGNRISPTYEAKGVTPRCFFCIHVNGDSVCYEV
                     ECTNIPPPKKAAIKVRN"
     gene            11245..>11541
                     /locus_tag="PK977_06990"
     CDS             11245..>11541
                     /locus_tag="PK977_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17894.1"
                     /translation="MQRFSLIFLSVVLTATTLQSQNNVTIKFSNTSLVTGNEAGRTSF
                     TSNEFIYARAELGKTVKDFFKIENPDDATSPHSLNFTFKMEFTDVKDGKNYTSNF"
BASE COUNT         3672 a         2214 c         2327 g         3142 t
ORIGIN      
        1 agatcactgg gtacagttgc cgaactagaa caatggctga aggatgccag tgagctggaa
       61 gcggtaatca gcgaagatgc ctgctggcgg caaataaaaa tgacctgcga tacagagaat
      121 aagcagctgg aagaggcttt caattttttc atgatggaaa ttcagcccat gatccagccg
      181 tatgctgata agctgaataa aaagctattg gcttgtccgc atttgtcctc actggatcaa
      241 tcaaagtatt ttacttacct gaggaatgta aaaaagaata tcgagctatt cagggaagaa
      301 aatatccagt tgaatgctga actgaatgta atgcagcagc aatatggtgt tattgcaggg
      361 aaaatgaccg tacaagtaaa cgggcaggag tacaccttac aacaggctgc taaattttta
      421 gaagaccctg accgaaaatt aagagaagaa gtttacagaa aaataaatga aagaagactg
      481 caggataaag aggagttgaa tacacttttt tcaaacttgc tggaaaaacg tcatcgtgta
      541 gccttaaatg ccggctttgc aaactaccgt gactttcgat ttaaagaact cggacgtttt
      601 gattatacca aagacgaatg ttaccagttt catgaagcgg tgaaactaca tgtcatgccc
      661 ctggttaacc agatatatga agcaaaaaag aaaaaactag gcctggatac gcttcgtccc
      721 tgggatgtgg aagcggaacc tgcaggtatt caaccgcttc gtccttttaa aacaggcgag
      781 gaactgacag aaaaaaccat cgcctgtttc cgtgggctaa aaacattttt tgctgattgc
      841 cttcaaaaaa tgaaggccat gggtcacctg gacctggaaa gccggaaagg aaaagcaccg
      901 gggggatata attgcccgct ggcagaaagc ggtgccccgt tcatttttat gaatgctgcc
      961 ggcacccttg atgatgttac gacgatggta catgaaggtg gccatgccat tcattctttt
     1021 ctctctcaca accttgaact gaccggtttt aaggaatacc ctactgaaat tgccgaagta
     1081 gccagcatgg ctatggagtt attcagcatg gataaatggg atgtgttttt tgataatgcc
     1141 gatgaactca aaagagctaa agaacaacag ctggaaagag taattaccat cttcccgtgg
     1201 attgccacta ttgataaatt tcagcattgg atttatgaaa atcctgtgca cactgaagag
     1261 gatagagcag gaaaatggat ggagatactt aacgagtttt catcgcccat agtagatgtg
     1321 caaggtttgg acgaatatcg tcgctatggc tggcagcgcc aactccacct gtacgaagta
     1381 ccgttttact atatcgaata tggtattgcc cagctgggag ctatcgggct ctggcagcag
     1441 tacaagcaaa accctgcaaa tgcagttaat aattatataa cagcgcttag cctgggtggt
     1501 acccgtacct tacctgagtt gtttaaagct gccgggctgg acttcaattt atctcccggc
     1561 tatattaaag ggttaatgga gtttgttaaa aaggaagccg atagcttgta aatagtaaaa
     1621 ataccacaaa agtggtatgg cctaaattcg gaaatatcaa agtgagaact tacttttgat
     1681 tcagattaat aatccgataa aaccggattt tacatagcta ctaatcagag ccttaaccaa
     1741 aagtcccgct gtttctacac cggggcttta ttttttactg gcagtttttg cagatacctt
     1801 ctaccaccac ttccacatga ccggcagaat atcccctggg taacttaatg tcgggggtca
     1861 ccacatcatc taaacaatag gttttattac aatgattgca aacaaaatga atatgatgat
     1921 catggtggtg tccttcgccg caatcatctt tacataatgc ataacgaata gaattatcag
     1981 cagtcgggat aatatgaatg atgcctttgt caacaaatgt ttgcagggta cggtaaacag
     2041 taacccggtc aaatttttcg ccagctcctt tttcaatatc gccatgtgcc atggcgccgg
     2101 tatgctccag gaaaagctgt aatattttcc tccggctgtc ggtaacacta agcctgttac
     2161 gcttcaggag cgattcaatc ctgacggcta tctcatttgc tgattgcttt ggcataaatt
     2221 attcctgcaa gtattttttt atatcgccga tcaattcttt tacttcctct tctttcaggc
     2281 catcataaat ctttcttaca tccccgtttc ggtccaccaa tgcccagaat tgggaatgaa
     2341 taaactgatc ttcgatactc tttaaattgt tggcggggtc atcaatggta tagcttaccc
     2401 ttgccatgtt atagagacta tcctttctac cggttaaaaa aatccatttg tccgtattca
     2461 ctcctaagga atctgtataa tgtttaagtt gtgccaccga gtctgtttcc ggatcgcagg
     2521 tatgagaaag gatcagaaaa tctttttcat ttttaaactc ctcatagact gttttcagat
     2581 agttattcat tgtagggcaa atcgatttgc aggtagtaaa gaaaaaccct gccacatata
     2641 cttttccctt aatatccttc tcagtaatgg tctttccatc ctggttagta aaggcaaaag
     2701 gccttacaaa actgatcggc gctacccctt ttttggtaaa gccgggaatg acctgggcca
     2761 acacaaaata ggagccaaag gccagtaaaa gaaaaaagct cagataaaaa acacccttct
     2821 ttgacatact ctcaccatta attgagcggc aaagttacga cagcaagtga taattatttt
     2881 gcaactgtgt tgctttttta tacttttgct gtttgcaatg aaattaaaga tcaattggga
     2941 cgctttgggc attggggcca ccctggcctg tgctattcat tgtgccatgt tgcctttgct
     3001 gatgagcagc ctgcctttat ttggaattaa tatcattcat cataccgggt ttgaagttgg
     3061 gatgatcctg ttggcactgg cgatcggcac ttattctctc tggcatggtt accgtaagca
     3121 tcaccacagc attttgccga tcgcaatatt ttatagtggc tttgctttcc tggtgctaaa
     3181 acagtttttc ttgcattaca gcacctggct attaattccg gccgttttgc tcattatcac
     3241 cgcccacctc atcaattata aatcatgccg ggtgcataat catgctcatt ccgatgactg
     3301 caatcattag tatgtaagat tgtttacttc agattgacta ttttcattac agcaactttc
     3361 tttagaatat tcatttttaa ttccccttac atgaaaaagt tttcttcttt cattgtctgc
     3421 ctgttttttg gtatttccat attggcccaa agcaaacagg agaaatctat acttgccatt
     3481 ttaggtaagc aaacccaagc ctggaaccgg ggagatattg aagcattcat gaatggatat
     3541 tgggaaaatg attctctgat gttcattgga aaaagcgggg taacttatgg atggaaaaac
     3601 actctcaata attacaaaaa aggataccct gatactgcag caatgggtaa acttacattc
     3661 actattatcc aggtaaaaaa actaaccgcc aaatattatc atgttaccgg aaaatggttg
     3721 cttaaacgat cggttggcga tgcaggagga cactatacgc ttttattcag aaagatcagg
     3781 tggcgatggg ttattatcag tgaccacagc agttagcatt tgttnnnnnn nnnnnnnnnn
     3841 nnnnnnntct tccatatttt gaaaaagtag cgaagcagta aaaagaatgt aattgccatc
     3901 aatacataaa aaataactgt ataaatgccc tgtttatatg ctaactggaa gtacaaacca
     3961 gcagtggcac aggccatcaa ccacaataag ccccacgaaa cagtatttat gatccgtaga
     4021 aaaaattttt taacctccgg gtcccagcct tgctccattc tttcaaattg aaaccgaaag
     4081 gtacattatt tcaaatgccg gtaattgttt gtttaacgaa gcagcaggtt tttaaaatca
     4141 gaagagtcgc cgttaaacac attgaaatca acgggtgaaa ctatcccgtt aacccttcct
     4201 tcatcgctgt gctgccagaa tacccatccc ctactgatgc ggggttgctc cggctggtaa
     4261 taatgcgcca cccatagcgg gtagttgtca aaatcactgc ccaggttacg gttataaaaa
     4321 tctacattgg tataaataat aggttttacc ccgtaatggt tctctgcgat gtcgagccat
     4381 ttttttactt ctttctttaa ttgttccgga gatgttccat tcagttgctc cacatccagt
     4441 accgggggaa ggtcgcccgg ttccagttct accttatcaa taaaattctc cgcctgtaag
     4501 cggccgtctt tcgatgcaat gaaaaaatga taggctccgc ggaagattcc ggcatctttt
     4561 gacctcttcc agtttctttt aaaatgtgga tccgtattac caataccctc tgtagcctta
     4621 ataaaagcaa aacccaattg aatatcctgc acattcatcg cctttacttc ctcccaggca
     4681 atagaagact gatatttgga aacatcaatg ccgtgaatag tataacctcc gggaataaca
     4741 atgccgaatt cggggtaacg tacagacctg gctttgcggt atgttttcca ttgccagaat
     4801 aaccagctgc ctgttatcag aacaacagca cccattacta atagcaagtt gcggcgtagt
     4861 ttatatttct ggcggcgggt aagcatttaa taaaagcatt gcatgcaaat gtaaaatgtc
     4921 aaaccttatt gaattgccaa agtttgaaaa aggaatttgt atatttagat accccaccat
     4981 gagctatgct attgaacagt tctatcgcct ctttcagggc gcattgtttt tccagggaat
     5041 tttctttgga attctctttt tcttgacaaa gcgaaaagat atcatgtggt acgctttgta
     5101 ccttttagca gcggcagcca atttctttat taatgcaagc ggcacatttc ttaacattga
     5161 tgannnnnnn nnnnnnnnnn naaaacctgg taggtaagga agcagcgaac aatgcggtaa
     5221 aatacagcgg ggcatcagaa ctggttatca gaactagtaa atcaaaagga caactagcat
     5281 taactgtatc agataatgga aaaggtttta atccagaagc agggtatgca ggtaatggaa
     5341 taaacaatat gaaaaaaagg gcggaagaaa ccgggggtca attatcaata taatcaggtg
     5401 cgaaagcagg gaccgaaata agttattgct gcaaaatcac ctgaagggtg attgtttatg
     5461 gatataataa ttgcaatttg tgttcagatt tttttgaagt aattaataaa tgtttagtaa
     5521 gcaattaaaa tatttagggc tattgctatg cctgaattgt gtcattgcgt cagcacaata
     5581 tgtcgcgccg gacagtctga agaaaattat tgccactgca aaaaaagaaa cagacagaat
     5641 tgatgcgatg aattggctgg cattcagata ttatcaaaaa ttagatacaa gcagttgtcg
     5701 catttgggca gagagggcac ttagttcttc agtaaaaatt ggctataaaa agggtgaggg
     5761 ctttgcttat attaacctgg gaaacataaa ttatgcttat ggacctgatt ctttggtaac
     5821 aaaatattac caacaagcaa aaaaagcact tgaaactgcg ggagataaag gactccttgg
     5881 cattgttcat agtaatttgg cctcgcattc tatgttcaaa caatcatacc ttgaatcatt
     5941 aagagagctt aataaatccc tgaggcttca acaggagtca gggtttcaat ttggtcttgc
     6001 acatagctat atgtcaatcg gaacctatca ttttttaatg ggagattaca ttgaagcaga
     6061 aaagaatctt tcaaaatcag aacacatgtt cagagtgcaa aatgaaaaca ccttgctatc
     6121 ctattcttat tatttcggag gcctaactta ttctgctctc aaaaaatata ggcttgcaaa
     6181 agcacactta gatatggcaa gggaaatttt taaaaattct ggtgaaaccg tttggtttga
     6241 aatagagtat taccggtgtg ttgcgatgat ttgtttggaa agggctgact cttgcctgac
     6301 agccaaaaat aaaaccctgg cggttgaaaa ctttatgtta tcggaaaaga atttaaatat
     6361 agcagttcaa aaaaatacca ttaatgacta tgatcagttt ggtttgatct ataataatct
     6421 tggcttgcta tacctatcat ggaaaaaata tgcactttca aagaagtttt ttcttaaatc
     6481 gctagagtca gctcgtataa cccgggacaa aagaattttt gatggatcac atgatgggct
     6541 ttcgcagatt gacagcatta acaatgattt tgaatctgct ttagctaatt ataaaaatca
     6601 tatactttat cgtgatagca ttaatagcga aaagaatatc cgggatattg aacgttataa
     6661 aatagagttt gaaactgaaa aaaatctgga tgatatcaaa ctgcttactg ccgaaaacga
     6721 attgaatact gctattgttt ctaaacaaaa gcaacagaag aattttgtat tcgcatgcac
     6781 tggtatttta ttactggcag gtatttacgg gtttcaacgt taccgcaaca agagaagaat
     6841 acaaagtcaa caagagattg cagaagagcg gcaacgcatc agccgtgacc ttcatgacga
     6901 aataggcgcc accctcagcg gtattgctat gtacggacac atggtaaaaa acagcctggg
     6961 aaacagtaat acagatatgg ctgtgcaatc agcggatatt attcaggaat ccgctacaga
     7021 aatggtaaat aaactgaatg atattgtatg gcttatcaaa ccgcaaaatg agtccatgga
     7081 agccctgatc gggcggttaa atatatacgc tgctgaaatg tgtgccgcca aaaatattgt
     7141 tccggatatt caggttaccg gtgaggcagc aggcagtgcc ccgccactgg agcaacgaaa
     7201 aaatatttac ctgatatgta aagaagctat taataatgcg gcgaaataca gcggggcaac
     7261 cgtgttaacg atgcggttct tcgttgaaga aaaatggctt catatttcca taaaagataa
     7321 cgggcaaggc tatgaccctg agaatagtaa aaatggtaat ggccttgaga atatgcaaaa
     7381 cagggccaga caaattggcg cctctttaga tatactcact gcaccgggca atggctgcgc
     7441 tgttgaacta aagctaaaaa tcacccaata gggtattgat taggaaaaat gaaagcctca
     7501 actttatact tatggatatt aaggtagcca tatttgaaga tggcaaactg atcagggaag
     7561 cattgcaaac catattaaat ggcacacccg gctatgcctg caccggggca tttagcaatg
     7621 gcagcaactg ggaactggat atacgccgaa ccaatcctga tgtggtgcta atggatattg
     7681 aaatgcccgg gcttgatggt attgaactga cccgccttat ttcgcaaaaa tttgaaggaa
     7741 taaaagtatt gatacagacg gtatttgacg acagtcagaa aatctttaat gcactttgcg
     7801 caggggccag cggttatatt ttaaaaaatg atccgcccaa taaatattta gaagcgattg
     7861 ccgaagtata taacggcggc tctttcatga atgcttcagt agccaaaaaa acactgaact
     7921 tttttaccca aaaaaatatt atcctcacat cacccgaagg taccgattat ggcctaagcg
     7981 aaagggaaaa ggaaatatta aaagagttaa ccgaagctca gcccctgaaa aatattgctg
     8041 caaaacttta catcagcctg gaaaccgttc gcacccatgt aaaacatatc tatgccaaac
     8101 tgcatgtagc taaccgcacc gaagcagtaa tgaaggcagt gcaacaggga attgcctgat
     8161 ccaatttaat ctactattaa aatcacccga tgggtgattg ccagcgcaag atcattctaa
     8221 tagcttagca taaacctctt ttatgcaaaa gcctatctat ttaatagcct tgccttttat
     8281 ttgtttcggg atcacattta tcatttcctc atgctccggc aacaaaacaa atactcaacg
     8341 ggaagaaaaa aaggaaacaa cccgtgacaa gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnna aattcccaat agcagcctgt acattattcc tcctgccggt tttgcggttg
     8461 atgaattgtc tggcacactg gcacaaacaa atggtacagc ccattatatg caaatgcaaa
     8521 tactggccgg ctacactaag gaaaagttgt tttcggagtt taaatctgag gcagataaaa
     8581 attttcccgg aagctggaaa gaggaagccg taagcattgc agggcataac gctaccattt
     8641 accacaataa aacagcagcc tattcgcaat actatatggc ttttgccgat ggctatactg
     8701 acgaaatgat tatagccaac tatgaagaaa gcgaagcggc cacgggtaag gaaatgtatg
     8761 aggcgttaaa aacagtggtg gtgaaaaaat aaaacaacta tctatgcaaa attcaatact
     8821 aaaatcagtt gcaactgttt gcttattgtt ttttatccac tttgccaatg cacagataaa
     8881 aaacctgcat ccacaatccc ataaaattcc ggacagcctt gttatggata aagtgactgg
     8941 aaaggcttca gtaagatatg tagatgaaaa gtatatgaaa gatggctgga atgtattcag
     9001 ccatcagccg ggccggttta tctatgtaga ttataaaaat ggcgttgtca ggggctttta
     9061 tacttctgat acatcaggta agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaggggcg ataaagaatg tgaaacattg
     9181 ttttcatctg acgagtgcaa ggataacctt gtatgtgtag gcaacacctg tatgtgtacc
     9241 aggccttatg atgcgaaaga agtaataaag gagataaata aggctaaatc acccaacggg
     9301 tgattgttta ctgcaacagc tatcaataag tttgatgcac tatcatctga aaccatttgt
     9361 tatgaaactg gttaccataa caaccatttt agccatttta ttttttattg gcgcaaaagc
     9421 acaaacacaa aaaattgaaa tcaagggcag taccattaaa ccaatagaag gaacggaatt
     9481 gaaaaatggc caaataatga taaaacccgg ctatgaagca aggctaagnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnngct aagcgataac aggccaacaa ctacaggcca
     9601 cttttcttgt gtctgttttg tagttaccgg ggaatgtttt cctcagatta cccccacctc
     9661 atttagctgc gcatccagca atcaaaaacc atgcatggta gaatgtgaag tggttgaaca
     9721 aaaaactgag ggccctgata gtaaaccctc ttttcaaaaa ccgtggaaaa aggtggcaga
     9781 caggcggctg cagccataat acatttttga aaatactgaa tattaaacgc aaatgacaaa
     9841 ccttatctac tcacttactc ttttgctttt attgaagcaa actgaaattg tacctatcat
     9901 aaaaaatgct gcacaaccag atttgatagt taaagcctcc catttcagat tagatgcggc
     9961 caataaaaga atatacttta aagcagagat ttcaaatatt ggcgatgcac catgtactat
    10021 acatcccggg cccggtggtg gatacggtat tcaaacttac ttatcagcag ataatagtat
    10081 tggaaacgat gtggtgagcg gcggcagggt gtttgatgaa ttatttacgt ttacacttca
    10141 gcccggcgaa agcagattgc tggatggaga acattcagcc ggttatagtc aattaggaag
    10201 tttatcaggc tatccttacc tggtagttga actgcaatcg cctaatattg aaagtaacag
    10261 aacgaataat aagtctattt acaaacatgg tctgccccga aaaaaaggag agggacttga
    10321 agcgccaagg cccgacaata taccaacgca attagcgcca aaggctattg atttaaagtt
    10381 actgataagc gatgttacca gccaattagt tacagaacca gaaacaggaa agcagtataa
    10441 ggaatatact tacacagcca ctattaaaaa tattggcaac atcaatgcag ttattgattt
    10501 ggagaagcca atcagcctgc aatactggaa aacaacttct tgtaccgata catcgggtat
    10561 gacatctatg caacgccccg aaaaccaaca tgcagctttt gtgctggagc cgcaaaaaaa
    10621 cttttcattg ccattgcgaa aagtaaggca aaagccagat gctataactc cgctgctcgt
    10681 acaactggta tatgatgtat ctgaaaaaaa taaatggaac aattttgcct gtttgggaaa
    10741 ttgaggcggt tgacaaaata ccccaacggg tgattgttta cggctattgc tattaatagg
    10801 tttgataagg gtttgccgaa aacttaaaaa agagtagacc acaaataaaa ctaaattatg
    10861 aaaaagatta tcctgatggc attcgtcatt tgtcttgctt tttttaacgc taaagctcaa
    10921 acaccagcta ctaatattaa cgcaaaagtg gaaacaagca gaaaggcgaa tggagacgtt
    10981 ttgatacaca ccgaagaggg aaagtttaaa gtatatgctt cattcaaaaa gagtaaagca
    11041 accggctatt atgcggttga tatgacgggg aatagaatca gcccaacata tgaagcgaag
    11101 ggagtgactc cccgatgttt cttttgtatc catgtaaatg gtgattcagt atgttacgag
    11161 gttgagtgta ctaatattcc acctccgaaa aaggctgcta taaaagttag aaactaagaa
    11221 tcactttcta ctaaacctca cacaatgcag agattttctt tgatatttct ttcggtggta
    11281 ttgactgcaa caactttgca gtcgcaaaac aatgtaacta ttaagttcag caatacttcc
    11341 ttagttacag gcaatgaagc tggtagaacc agttttacgt ccaatgagtt tatttatgcc
    11401 cgggccgagc tgggaaaaac cgtaaaagat ttttttaaaa tagaaaaccc ggatgatgca
    11461 acatcaccac actcattgaa cttcaccttt aaaatggaat ttactgatgt aaaggatggt
    11521 aaaaactata cctcaaattt t
//
LOCUS       DAORLS010000068        11536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_2992_length_11536_cov_2.229945, whole genome shotgun sequence.
ACCESSION   DAORLS010000068 DAORLS010000000
VERSION     DAORLS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2992_length_11536_cov_2.229945"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>253)
                     /locus_tag="PK977_06995"
     CDS             complement(<1..>253)
                     /locus_tag="PK977_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17895.1"
                     /translation="GLSINKNAEKPLKVKSFTMAIRNYENFIKDSTYSIQFDSILFRN
                     DKLTLSKFVFNKLNDGKVSNTFSIPQFNLSGLSWDDLVFE"
     assembly_gap    254..275
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<276..1365)
                     /locus_tag="PK977_07000"
     CDS             complement(<276..1365)
                     /locus_tag="PK977_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17896.1"
                     /translation="MPVRFIKRTIKITGIIIGILAVLALGFHLWFRAHAKGIIEDLVE
                     SRSKGKINLKIEKLRFGYFSNKIELEKAVFFNTDSASTATSYKFYIDKLKMKGKGLIA
                     LLFSKELFIDSLSLTNPVIEVNRVSMADKSGKRKEISIPEEMGKIYSSIQDAINVLQV
                     KKFQLENATFILANKTEPDQKPVRISNLHLDIDNLQVDSSKSRVNEKMFFSDNITLRT
                     QNQDITFPDGRHKLSFSRFRINLKNKLVEFDSCTIAATRTDSTASSFNIFFDALLMTN
                     IDFDTLYRHEVIKADSVYCVNPRFNLTAVSTKKTGLKKSTPKLENIIQQLTGDLDLNH
                     VVVANADFNILTIKDGIPNSFVFSKNNFE"
     gene            complement(1470..>3534)
                     /gene="metG"
                     /locus_tag="PK977_07005"
     CDS             complement(1470..>3534)
                     /gene="metG"
                     /locus_tag="PK977_07005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRF17897.1"
                     /translation="ANGLKHVGHLAGAYIPADIYVRYLRAQKRDVVFVCGSDEHGTAI
                     PIQATKEGTTPRAIIDKYHEAMKQDFEDLNMSFDIYHRTSESIHHETAQEFFTYLHDR
                     GELETKETEQYFDEEAKTFLADRYIKGTCPNCGSDRAFGDQCETCGRTLSPDELINPV
                     STLSGKAPIKKKTTHWYLPLGKHEQFLREWILDQHKDDWRTTVVGQCKSWIEGGLQSR
                     AVTRDLDWGIKVPLKEAEGKVLYVWFDAPIGYISATNQWAIDNNKNWEPYWYDKDTKL
                     VHFIGKDNIVFHCIIFPIMLKLHGNILPENVPANEFLNLEGDKMSTSRNWKLDMRDYI
                     NDFIKKENGGGQCVDMLRYYLTQIAPETKDSEFTWKGFQDAVNSELVSIFGNFVNRTF
                     VLMHKLCNGKVPVFHNDKIDELDKQLISDIAAVKEKVETLLEQYKFREALFEVIDLSR
                     KGNKYMQEKQPWIVAKTLAEKPADQQLIDNCLHLCLQLTANLAILVNPFLPNTAKKML
                     YLMKVVDKMLNWENAGKLKLLSPGYSLRAPELLFRKIEDSEISEQIEKLNAGLIKPEA
                     ITKMEEIKQPAGKAQIVYDDFAKLELKAATVTACEKVEKADKLLKLEVDLGTEKRTIV
                     SGIAQHYKPEEMVGKQVIVVTNLAPRKMKGIESQGMILTAENSDGKLQLLKPENPVAP
                     GSNVS"
     assembly_gap    3535..3544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3674..4783
                     /locus_tag="PK977_07010"
     CDS             3674..4783
                     /locus_tag="PK977_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HRF17898.1"
                     /translation="MRKIILPLLILFIQQAKANDGVFYAMGNTLVPLKETTIRLKKEI
                     LNLDRKGDWMQVNIYFEFFNPGAERELTVGFVTPPAFGDVSDEEAKHPFIKDFMVMVG
                     DRLLPFKIAKMEETGFKVSSKVSEGYDFVYHFTVRFAKGVTVIRHSYLYKGGGSVEAR
                     QDFDYRLTTGTTWANSAIDDFEMNIDMGDDSYFSVPASFSIAPASWQIAGIGRLSKTN
                     FSLPYMPEDKDRLKMVYLRKGKLQLRASAFKPVQDLTINFWNLHNEINLWCDNKEQND
                     FKGMMDMIWGDSVEATVSKLTDNELRLYRNLNYARMGYDFKDESLKRIFSKYNWYLPD
                     PLLKPDNVPDYYVTKELQQLIIAEEKRRKLKTDIP"
     gene            4780..>5034
                     /locus_tag="PK977_07015"
     CDS             4780..>5034
                     /locus_tag="PK977_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRF17899.1"
                     /translation="MIKIPPYLKKGDTIGLVCPAGYMPVEKVQTCLDVLQEWGYRVKI
                     GKTVGGGSATYFSGTDEERLTDFQQMLDDDAIKAVLCARGG"
     assembly_gap    5035..5089
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5090..5698
                     /locus_tag="PK977_07020"
     CDS             <5090..5698
                     /locus_tag="PK977_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRF17900.1"
                     /translation="RKHPKWIVGYSDVTVLHSHIYSHFYISTLHAPMAGAFNDDGFKN
                     EFIRSLKNALEGKKLKYKCEVHDYNRKGEAVGELVGGNLALLAHLVGTDSDIKTRGKI
                     LFLEDVGEQLYNIDRMLHQLKRSGKLARLAGLIFGGFTESKDTERPFGNSIHEILRDI
                     VKEFDYPVCYDFPISHTDRNYALKIGVGYKLKVGKSKVTLEE"
     gene            complement(5701..>5999)
                     /locus_tag="PK977_07025"
     CDS             complement(5701..>5999)
                     /locus_tag="PK977_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17901.1"
                     /translation="SQPNFDLFVLLEEKKRADSSRVQVVYFITTKKDGQFIAKLEAAV
                     NGERKQSGYNITSLLCKDYKIVQDSRITVNDKSYTDMTYYKINGGGRFILYPKE"
     assembly_gap    6000..6009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6010..6251)
                     /locus_tag="PK977_07030"
     CDS             complement(<6010..6251)
                     /locus_tag="PK977_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17902.1"
                     /translation="MKFRLFILMLVLSLCARTQMTLSYEQLLENTPEISFTDSLLIDA
                     QIKEIPALDSASVKKWFNAVLPAGSKNRLKNRTYATV"
     assembly_gap    6397..6422
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6423..7122)
                     /locus_tag="PK977_07035"
     CDS             complement(<6423..7122)
                     /locus_tag="PK977_07035"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF17903.1"
                     /translation="MTKKKFIKWLKITAIIYVAGGIALYFLQNAILFHPVRLDRTYNY
                     TFPEPHKDISIPLNAKDTLNLVDFASTDTVTKGVVLYFHGNKRNISWYSKYIPYFTRQ
                     GYQVLMIDYPGFGKSTGKLTEKKLYDWALQVYRIAIKRFAADSIIIYGKSMGTGIAAQ
                     LSSVRDCKRLILETPYYDFPAVVQHYLPIYPVRLLLNYKLPTYQYLQDVKAPITIFHG
                     TKDRIVTYKNAKRLS"
     gene            complement(7193..>7801)
                     /locus_tag="PK977_07040"
     CDS             complement(7193..>7801)
                     /locus_tag="PK977_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17904.1"
                     /translation="VVVLPMQQQGGYVYSSYTIGGNNLDDQLIQNLQYSSGNEFVRFY
                     SAWDARSQNIRVDQEMQMRMAQVNIGRYIDNQSKRKVTKEIVVKEIVYKPDSVVREYA
                     KISADITTTRRTLHSNATLLITVRDEHGNRVWSDDVSAVYNWSTEFSTFKGDERALNE
                     SDKNLVNRRRDFPPGENEIIRCILEEIGNNALYRIKNYFNRL"
     assembly_gap    7802..7815
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7816..8341)
                     /locus_tag="PK977_07045"
     CDS             complement(<7816..8341)
                     /locus_tag="PK977_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17905.1"
                     /translation="MKWKLYTLLFTGISLLAISCKTASKMYEKGNYDEAVELAAKKLQ
                     KDPDDPKLLNIIQSSYRYAVNDHESRISDYAQSNNELKWEWMYNEYVSLQRMYDAIYK
                     APAVFDLVKPINYSSNIITYSEKAGDVRYDRGIAFMQRYDKQSYRHAYREFQAALRFK
                     PGNRDATLKMREAYE"
     gene            complement(8440..9228)
                     /locus_tag="PK977_07050"
     CDS             complement(8440..9228)
                     /locus_tag="PK977_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF17906.1"
                     /translation="MPNLIWYILAGITSLSILAYLLQDRLIFKPEKLKQDFEFKYDAP
                     FKEYFFDVEPGVRINGLHFYRDNPKGLILYFHGNTRSIKGWARYAKDFYRYDYDVVLV
                     DYRGFGKSTGKRSEKEMLGDMQFIYDKLKEQYAENHLIVYGRSMGSGFATKLASDNNP
                     RYLILDAPYFNFLKVIERFLPILPVRIVLRYHLRTDIWITKVNCPVYIIHGTKDWLIP
                     IRHSESLQKINPRKITLIRIHGGGHNNLPSFDEYHNFIRDILKY"
     gene            complement(9285..>11182)
                     /locus_tag="PK977_07055"
     CDS             complement(9285..>11182)
                     /locus_tag="PK977_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRF17907.1"
                     /translation="SPLYVFNSIIYDRVVDENLIIGTLSCIIWTLTLQTTIKYVILVL
                     RADNRGEGGTFALFALVRRRKKWLVIPAMIGGASLLADGIITPPISITSAIEGLRELP
                     TLGIHEGSDTVVIIVLSILVLFFFMQQFGTASIGSLFGPVMFVWFTMLLVLGALHVFD
                     DLQIFKAINPYYAVYFLKNYDGGFWLLGAVFLCTTGAEALYSDLGHCGRGNIRTSWIY
                     VKTCLLVNYFGQGAYILSHYGGKEINEATKASMGINAFYDLMPHWFIIPGVIIATTAA
                     IIASQAMVSGAFTLISEAMRLNLWPKLRIRYPSEAKGQLFIPTINFIMFLGCVGVVLF
                     FRQSSKMEAAYGLAIIVTMLATTILFANYLVLNRIKSIWIYIFLGLYLIIELGYLVAL
                     MVKFTHGGYITLLIGMIMFFVMYIWYRARKIKNRYVEFVRMEHYVPKIQELSNDKSVP
                     KYATHLVYMTSANNPKEIEHKIIYSILNKKPKRADIYWFVHIDTVDDPYTCEYKVEHI
                     IPNDIIRVDFRLGFRMEPRVNLMFRKVVEDLVANKEVNIISRYESLASTGTVGDFQFM
                     VMEKYLSQDNELPFIERVIMKFHFWLKEHSLSEEKGFGLDLANVTVEKFPLIVAPVTN
                     LKLKRVE"
     assembly_gap    11183..11198
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3207 a         2243 c         2372 g         3561 t
ORIGIN      
        1 ttcaaaaaca aggtcgtccc aggagagacc agataagttg aattgtggta tgctgaatgt
       61 atttgagact tttccatcat tcagcttgtt aaaaacaaac ttgcttaaag tcagtttgtc
      121 atttctgaaa agaatactgt cgaattgtat gctgtatgta ctatccttta taaagttttc
      181 ataattacgg atggccatag taaagctttt aaccttcaat ggcttttcag catttttatt
      241 aatactgaga ccannnnnnn nnnnnnnnnn nnnnntctca aaattattct tgctgaatac
      301 aaaagagttt ggtatgccgt cctttatagt aaggatgtta aaatcagcat tggccaccac
      361 tacatggtta agatcaagat cgccggttag ttgctgaatg atgttttcca atttaggtgt
      421 acttttcttt aaccctgttt ttttcgtact gactgctgtg agattaaatc ttggattcac
      481 acaataaact gaatcagcct tgatgacttc atgccggtac agtgtgtcaa aatcaatatt
      541 tgtcatcagc aaggcatcaa agaaaatatt gaaggaagag gcggtactgt cggtccgggt
      601 ggcggcaatg gtacaactat caaactcaac cagtttattt tttaagttga ttctgaatct
      661 gctgaagctg agtttgtgcc ggccatccgg aaacgttata tcctggtttt gcgttcgcaa
      721 tgtaatgttg tcgctaaaaa acattttttc attgacacgg gacttgctgc tgtcaacctg
      781 caggttatca atatcgaggt gcagattact gatgcgaacc ggtttttgat caggttctgt
      841 cttattggcc aggatgaatg tggcattttc aagctggaac ttttttacct gcaaaacgtt
      901 tatggcatcc tggatagagg aataaatttt gcccatttct tccggaattg atatttcctt
      961 tcttttcccg gatttatcag ccatacttac cctgtttact tcaattacag gattggtgag
     1021 agatagggag tcaataaata attctttact gaataaaagg gcgatcaggc ctttaccttt
     1081 cattttcaat ttatcaatat aaaacttgta agaggtagca gtagaggcag agtcagtatt
     1141 aaaaaaaaca gctttttcta attctatttt attactgaaa tagccaaaac gcagcttttc
     1201 aattttaagg ttaatttttc ctttggagcg ggactcaaca agatcttcga taatgccttt
     1261 ggcatgtgcc ctgaaccaaa gatgaaaacc caacgctagc acggcaagga taccgatgat
     1321 aatccctgta atttttattg ttctcttaat aaagcgtacg ggcaactttg aaagaatttg
     1381 gaagtgaatt taccaattta taagctgtgc ttaatgaaaa agccccgctt tgtggtaaac
     1441 agggcagggc tattggagat ttttacgatt tagctaacgt ttgaacccgg cgctaccggg
     1501 ttttccggtt ttaacagttg taatttccca tcactgtttt ctgcagtcaa tatcattccc
     1561 tggctttcga ttcccttcat ttttcttgga gccaggttgg ttaccacaat tacttgtttg
     1621 cccaccattt cttctggttt gtaatgctga gcaataccgg atacgattgt tcttttttcc
     1681 gtacccagat caacttccag tttcaaaagc ttgtctgctt tttctacttt ttcacaggca
     1741 gtaacagtgg cggcttttaa ttccagtttg gcaaagtcat catagacgat ttgagcttta
     1801 ccggcaggtt gttttatttc ttccatttta gttattgctt caggtttaat caatccggca
     1861 tttaattttt ctatttgttc tgaaatttcg ctgtcttcaa tttttctgaa taataattcc
     1921 ggtgcccgca ggctgtagcc gggacttaaa agttttagct ttccggcatt ttcccaattg
     1981 agcattttgt ctaccacttt catcaggtag agcatctttt tggcagtatt gggtaaaaat
     2041 ggattgacaa gtatggcaag gttcgctgtt agctgtaagc aaagatgaag gcagttatca
     2101 atcagttgct gatcggctgg tttttcagcc aaagttttgg caacgatcca aggttgtttt
     2161 tcctgcatgt atttattgcc tttgcgggaa agatctatta cttcaaacag agcctccctg
     2221 aacttgtatt gttccagtaa ggtttcaact ttttctttta ctgcggctat atctgaaatc
     2281 agttgcttgt caagttcatc aatcttgtca ttatgaaaaa ctggtacttt gccattgcat
     2341 agtttatgca tcagcacaaa agttcggttc acaaaattgc caaagatgga aaccaattca
     2401 ctgtttaccg catcctggaa gcccttccag gtgaattcgc tgtcttttgt ttcgggtgca
     2461 atttgtgtca ggtaataacg tagcatgtct acacactgac cacctccgtt ctcttttttt
     2521 ataaaatcat tgatataatc ccgcatgtcc agtttccagt tacgactggt gctcattttg
     2581 tctccttcaa gatttaaaaa ctcatttgca ggaacatttt ccggtaaaat atttccatgc
     2641 agttttaaca tgatagggaa aatgatacaa tggaaaacga tattgtcttt accaatgaag
     2701 tgaaccagtt tggtatcctt gtcataccaa taaggttccc agtttttgtt gttgtcaata
     2761 gcccattgat ttgtggcact gatatagccg ataggggcgt caaaccaaac gtacaatact
     2821 tttccttctg cttctttcag cggtaccttg attccccagt cgagatcacg ggtaacggct
     2881 cttgattgaa gaccaccttc gatccagctt ttgcattggc ctacaacggt tgttctccaa
     2941 tcgtctttat gttggtccag aatccattct ctcaaaaatt gctcatgttt gcctaaagga
     3001 agataccagt gagtagtttt tttctttatt ggggctttgc cacttagtgt acttacagga
     3061 tttatgagtt catcaggact caacgttctg ccgcatgtct cacattgatc tccaaatgcc
     3121 cggtcgctgc cgcagttagg gcaggtgcct tttatatagc ggtcggccaa aaatgttttg
     3181 gcttcttcat caaaatactg ttctgtttct tttgtttcca gttcaccccg gtcatgcagg
     3241 taagtaaaaa attcctgtgc agtttcatgg tgaatcgatt cgctggtacg gtgataaata
     3301 tcaaagctca tattcaggtc ttcaaaatcc tgtttcatag cttcatgata cttatcaatt
     3361 atggcccggg gcgttgttcc ttctttggta gcctgtatag gaatagcagt gccgtgttca
     3421 tcgctgccgc aaacaaacac aacatccctt ttttgtgccc tcaggtaacg gacataaata
     3481 tcggcaggaa tataggcgcc tgccaggtgg ccaacatgct tcaaaccgtt ggcgnnnnnn
     3541 nnnnaaccaa atatcgtttg gggttgctca tcaaaaaact cactttaata gcctgcaaaa
     3601 ataccggaat agcgatcatt gcccaaaagc atgatttcag ataactttag ggcattacca
     3661 taaaccatta aacatgcgaa aaatcattct gccattgctc attttattta ttcaacaggc
     3721 aaaggccaat gacggggtat tttatgctat gggaaataca ttggtacccc tgaaagagac
     3781 cactatacga ttaaagaagg aaatactgaa tcttgaccga aaaggtgact ggatgcaggt
     3841 gaatatttac ttcgaatttt ttaacccggg tgctgaacgg gaactgaccg ttggctttgt
     3901 tactcccccc gctttcggtg atgtctcgga tgaagaagca aagcatccgt ttataaaaga
     3961 ttttatggtg atggtgggtg atcgactgtt gccgtttaaa attgctaaaa tggaggagac
     4021 cggttttaaa gtgagttcaa aagtttctga gggatatgat tttgtttatc atttcacggt
     4081 gcggtttgca aaaggggtta cagttattcg tcatagctac ctgtataaag gcggcggctc
     4141 tgtggaagcc aggcaggatt ttgattaccg cctgacaacc ggtaccacct gggccaacag
     4201 cgctattgat gactttgaga tgaatataga tatgggtgat gacagttact tttctgtacc
     4261 cgcctcattt agtattgctc cggcatcatg gcaaatagca ggtataggaa ggctcagcaa
     4321 gacaaatttt tcgctgccat acatgccgga agataaagac aggcttaaaa tggtttatct
     4381 gagaaaagga aaactgcaat taagagcatc tgcttttaaa cctgttcagg atttgaccat
     4441 taatttctgg aacctccata atgaaatcaa cctttggtgc gataataagg aacaaaatga
     4501 ttttaaagga atgatggaca tgatctgggg tgattctgtg gaggctactg tcagtaaact
     4561 cacggataat gaactaaggc tttaccgtaa tcttaattat gcaaggatgg ggtatgactt
     4621 taaagacgaa tcattaaaaa ggattttttc aaaatacaat tggtatttac cagacccgtt
     4681 attgaaacca gataatgttc ctgattatta tgttactaaa gaattacaac agcttattat
     4741 tgcagaagaa aagcgaagaa aattaaaaac tgatattcca tgatcaaaat tccgccttat
     4801 ctaaagaaag gagatactat aggacttgtt tgccctgctg gttatatgcc ggttgaaaaa
     4861 gtacaaactt gcctggatgt cttacaggaa tggggctacc gggtcaaaat cggtaaaact
     4921 gttggaggag gatctgccac ctatttttca ggtactgatg aagaaaggct gactgacttt
     4981 cagcaaatgc tggatgatga tgctataaaa gcggtgctat gtgcaagagg aggtnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gaaagcatcc
     5101 caagtggatt gtgggttata gcgatgtaac agttctgcat tcacatattt actcccattt
     5161 ctacatttct actttacatg cacctatggc cggggctttt aatgatgatg gctttaaaaa
     5221 tgagtttatc aggtcattaa agaatgcgct ggaaggcaaa aagctaaagt ataaatgcga
     5281 agtacatgac tataacagga aaggtgaagc tgtgggtgaa ctggttggag gtaacctggc
     5341 attgttagcc catttagttg gaactgattc tgacattaaa acgagaggta agatactttt
     5401 cctggaagat gtgggagagc aattgtacaa tattgaccgg atgcttcatc agctaaaaag
     5461 aagcggcaaa ctggccaggc ttgcgggatt aatatttggc ggctttacag aatcaaagga
     5521 tactgaaagg ccttttggga actctattca tgaaattctc cgggatattg tgaaggaatt
     5581 tgactatcct gtatgttatg attttcctat tagccacact gacagaaact atgcattgaa
     5641 aataggagtt ggctacaaat tgaaagtagg aaaatcaaag gtgacactgg aagaataggc
     5701 tcattctttg ggatataaaa taaatctgcc acctccattg atcttgtaat aagtcatatc
     5761 tgtataggac ttatcgttga ccgttatccg ggagtcctgt acaattttat aatccttaca
     5821 gagcagggaa gtgatgttat agccggattg ttttctttca ccgtttacag cagcttccag
     5881 tttggcaata aattgcccgt ctttttttgt ggtgatgaaa taaaccacct gcaccctgga
     5941 actgtcagct ctttttttct cttccagtaa aacaaaaaga tcaaaattgg gttgtgatgn
     6001 nnnnnnnnna ctgttgcata agttctgttc ttcagcctgt ttttactgcc cgccggcaat
     6061 actgcattga accatttttt tactgaggca gaatccagtg ccggtatttc tttgatttgt
     6121 gcatcgataa gcaagctatc ggtgaaactg atctctggag tattctctaa tagttgctca
     6181 tagctcagtg tcatttgtgt cctggcacaa agactcaaaa caagcattag aatgaacaac
     6241 ctgaatttca tccggtaaat atagacttgc ttgctgacgg ccacaatacc acatttgagg
     6301 tattttatgg cagtttcagc agcgaatcaa gcttactgat cgtttctggg tacgtaaaca
     6361 ggttgttatg gcttcctcct ttgatggtaa ccaattnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nntactcagc cgtttcgcat tcttataagt tacaattcgg tccttagtac catgaaaaat
     6481 ggttataggt gcctttacat cctggagata ttgataggta ggcaacttgt agtttagcaa
     6541 caagcgaact gggtaaatgg gtaaatagtg ttgtaccacg gcagggaagt cgtaataagg
     6601 tgtttcaagt atcagtcttt tacaatctct tactgaagac aattgggctg caatgccggt
     6661 acccatactt tttccgtaga taatgatgct gtcagccgca aatcgtttga tggctatccg
     6721 gtaaacttgc aaagcccagt catataattt tttttctgtg agtttgcctg tgcttttgcc
     6781 gaagccgggg taatcaatca ttaatacctg gtagccctgg cgggtaaaat aagggatgta
     6841 ttttgagtac caggagatgt ttcgtttgtt tccatgaaaa tacagcacca ctcctttggt
     6901 tacggtgtca gtactggcaa aatcaaccag gttgagagtg tctttagcat tgagtggaat
     6961 actgatgtct ttatgtggtt cgggaaatgt gtaattatat gttctgtcta atcgtacagg
     7021 atgaaataga atagcatttt gtaaaaaata cagggcaata ccgccggcaa catagatgat
     7081 ggcagtgatc ttcagccatt taataaattt tttctttgtc atgagcgatg caaagatgaa
     7141 aataaaaagg acatgcctgt catgtccttt ttactcttta ttcatggctt gtttataacc
     7201 tgttaaagta gtttttgatc ctgtataaag cattgttgcc tatttcttcc agtatgcatc
     7261 ggatgatctc gttttcaccc ggaggaaaat ctcttctcct gttcacaagg tttttatcac
     7321 tttcatttaa tgccctttca tcacctttga aagtgctgaa ttcggttgac cagttgtaaa
     7381 cagcggagac atcgtcactc catacccggt tgccgtgctc atccctgaca gtgattaata
     7441 aggtagcatt tgagtgtaag gtccttcttg tagttgtaat gtcagcgctg attttagcat
     7501 attctcttac cacagagtct ggtttgtaaa caatttcttt tactactatt tctttggtca
     7561 cttttctttt gctttggttg tcaatatagc gaccgatatt aacctgtgcc attctcattt
     7621 gcatttcctg gtccacccgg atattttggc tgcgggcatc ccatgctgag tagaatcgta
     7681 caaattcatt tccgctgctg tattgaaggt tttgtatcaa ctgatcatcg aggttgttac
     7741 cgccaatggt ataagagctg taaacatatc cgccttgttg ttgcataggc aacaccacta
     7801 cnnnnnnnnn nnnnnattca tacgcttctc tcattttgag ggtggcatca cggttgccgg
     7861 gtttaaaacg cagggctgcc tggaattcac ggtatgcatg gcggtagctt tgtttgtcat
     7921 atcgctgcat aaaggcgata cccctgtcat atcttacgtc acctgctttt tcgctgtagg
     7981 taataatatt ggaagaatag tttattggct taacaaggtc aaatacggca ggtgctttgt
     8041 aaatggcatc atacattctt tgcagggaaa catattcatt gtacatccat tcccatttca
     8101 gttcattatt actttgagca taatcactaa tgcggctttc atggtcattg accgcatagc
     8161 gataggagct ttgtataata ttcagcaatt tggggtcatc cggatctttc tgtaattttt
     8221 ttgccgccag ttcaacagct tcatcgtaat tgcctttctc atacattttg ctggctgtct
     8281 tacaggagat ggccagtaaa ctgattccgg taaatagtaa agtgtagagt ttccatttca
     8341 taaacacctc ctttttcata tagtacgttc aaatagcagt ccatgttaac agtgtgtctt
     8401 gtcaattata caatccttaa cagtcgggga gcaataagtt caatatttca gaatgtcccg
     8461 gatgaagttg tggtattcat caaaggaggg aaggttgtta tgcccgccac catgtatccg
     8521 gataagcgta atttttcgtg gattgatctt ttgtaaagat tcgctgtggc gtatcggtat
     8581 cagccagtct tttgtaccat gaataatata aaccgggcaa ttcacttttg tgatccagat
     8641 atctgtacgc aggtgataac gtaacacaat tcttaccggc agtatcggta aaaaccgttc
     8701 tatcactttt aaaaaattaa aatacggggc gtccaggatc aggtaccggg gattattgtc
     8761 agaagccagt ttagtggcaa aaccgctgcc catacttctg ccataaacga tcaggtgatt
     8821 ttcagcatac tgttctttta gtttatcata aataaactgc atatcaccca gcatttcttt
     8881 ttcgcttctt ttaccggtgc ttttaccaaa accacgataa tccacaagca ctacatcata
     8941 atcataacga taaaagtctt ttgcatacct ggcccatccc ttaatactac gggtattgcc
     9001 atgaaagtaa aggatcagcc ctttggggtt atctctatag aaatgcaggc catttatcct
     9061 gacaccgggc tctacatcga aaaagtattc tttaaatggt gcatcatatt tgaactcaaa
     9121 atcctgttta agtttttcag gtttgaagat cagccgatcc tgcaacaaat aagcaagaat
     9181 ggaaaggctt gtaatcccgg caagaatata ccagatgagg ttgggcaaat cagttttggt
     9241 ttacctgctg aaattaaaca actaaataga aaaagcgaat cttcttattc tacccgtttt
     9301 agtttaaggt tggtaaccgg cgcaacgatt aacgggaact tctccacagt aacattagct
     9361 aagtctaggc cgaagccttt ttcttctgac aggctgtgtt ctttgagcca gaaatggaat
     9421 ttcataatga ctctttcaat gaacggaagc tcattgtcct ggctcaggta tttttccatt
     9481 accataaact ggaagtcgcc aaccgtaccg gtactggcaa gactttcata gcggctgata
     9541 atatttactt ctttattggc caccaggtct tccaccactt tgcggaacat caggtttact
     9601 cttggctcca tacggaatcc aagtctgaaa tcgactcgga tgatatcatt agggatgata
     9661 tgttccactt tgtactcgca ggtatatggg tcatccactg tgtcaatgtg aacaaaccaa
     9721 taaatatctg cccgtttggg ttttttgttc aaaatggagt aaatgatctt atgttcaatt
     9781 tcttttggat tgttggcact ggtcatatac accaggtggg tagcatattt gggaacggat
     9841 ttgtcattgc tcaattcctg aattttggga acgtaatgct ccatacgaac aaattccaca
     9901 tagcggttct tgatcttcct tgccctgtac cagatataca tgacaaagaa catgatcatt
     9961 ccgatcagca aggtaatata accaccgtga gtaaatttca ccatcaaagc aaccaggtat
    10021 cccagttcaa taataagata tagtccaagg aaaatataaa tccagattga ttttatccgg
    10081 ttcaatacca ggtagttcgc aaaaaggata gtggttgcca gcatggtgac aataatagca
    10141 agaccataag cagcttccat ttttgaagat tgacggaaga ataaaacaac gccaacgcat
    10201 cccaggaaca taataaaatt gatggtggga atgaataatt gccctttagc ctcagaggga
    10261 tacctgatcc tgagttttgg ccataaattc agccgcatgg cctcactaat aagtgtaaag
    10321 gctcccgata ccatagcctg gctggcaatt atagctgcag tggtagctat gataaccccg
    10381 ggaatgataa accaatgcgg cataaggtca taaaatgcat taattcccat gctggccttg
    10441 gtagcctcgt tgatttcctt tccgccataa tgtgaaagaa tgtaagcacc ctggccaaaa
    10501 taattaacca gcaggcaggt ctttacatat atccatgatg tgcggatatt gcccctgcca
    10561 caatgcccca ggtcactgta gagggcttct gcacctgtag tacaaaggaa cacagctcct
    10621 aacagccaga agccgccatc ataatttttg aggaaataaa cagcatagta ggggttaatg
    10681 gccttgaata tttgcaggtc atcaaaaaca tgcaaagctc ccagcaccaa taacatggtg
    10741 aaccagacaa acatcactgg tccgaacagg cttccgatag aagctgttcc gaattgctgc
    10801 ataaaaaaga agagcaccag tatagaaaga acaataataa ctactgtatc gctgccttcg
    10861 tgtataccaa gggttggtag ttcccgcaat ccttcaatgg ctgaagtaat agagataggg
    10921 ggggtgatga tgccgtctgc cagcaaagaa gctcccccga tcatggccgg tataaccaac
    10981 catttctttc ttctacgaac aagggcaaat agcgcaaaag ttccgccctc accccggtta
    11041 tctgcccgaa gtaccaggat tacatatttt atagtggttt gaagggttag ggtccagatg
    11101 atacaggaaa gtgtaccgat gataagattt tcatcaacca cccggtcgta aatgattgaa
    11161 ttaaaaacgt agagaggtga tgnnnnnnnn nnnnnnnnat ctccgtaaat gattccaagg
    11221 gcaatgatca atccagctgc ggtgactctg ttggtcggtg ttttcacttt ctgtggttat
    11281 ggtttattcc tggattacct ggccgttcat aggttttgta atatggacag cagcgggcat
    11341 ttatcatggt ttgacagaag acaatgtaca aaggtatagg caaaaatggt ttattgcagg
    11401 caacctccaa aactttctat atcgtccttt gaaacaaatg gggataaact gtgtttttta
    11461 caacagggaa taaaaattgc agggctcatg taaaatgcta aaaaatggca tcagtataaa
    11521 atactcggac cggttt
//
LOCUS       DAORLS010000069        11503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3003_length_11503_cov_2.174528, whole genome shotgun sequence.
ACCESSION   DAORLS010000069 DAORLS010000000
VERSION     DAORLS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11503
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3003_length_11503_cov_2.174528"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="PK977_07060"
     CDS             complement(<1..135)
                     /locus_tag="PK977_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRF17908.1"
                     /translation="MLDFLKQPWPWYVAGPLIGLMVPVLLIIGNKSFGISSSLRHVCA
                     A"
     gene            complement(210..1214)
                     /locus_tag="PK977_07065"
     CDS             complement(210..1214)
                     /locus_tag="PK977_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF17909.1"
                     /translation="MWNTIRSNWQLVIIAAVVGIVALVEMAKVIRPSEKESPLTTTTD
                     SSWVTPSLFTDNELQGAERELVIYGEDIIANTARYFGPHGSVAAITNGMNCQNCHLNA
                     GKKTWGNNYSAVYSTYPKFRDRSGGMESIYKRVGDCFERSLNGTAPDSNSREYQAIYA
                     YIKWLGKDVKKGERPVGSGISKLNYLDRAADPVKGKAVYTAQCQSCHGANGEGQLSLN
                     GVSYEYPPLWGEHSYNDGAGLFRLSNFAGYVKSNMPFKQATHDNPKLTTEEAWDVAAY
                     VNSQPRPKKDLRNDWPDISKKPFDHPFGPYADGFSESQHKYGPYKPIIAKKEELAKKK
                     "
     gene            complement(1225..2919)
                     /locus_tag="PK977_07070"
     CDS             complement(1225..2919)
                     /locus_tag="PK977_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRF17910.1"
                     /translation="MSSLKNMDTNSRRDFLKKAALAGMGTTLGSVAIAGTTELSKKSE
                     PGTPGRFTILYTSDIHAQLHTHDEFFWENGKAVYRKRGGLAVLKTMINHFRKQNPANT
                     LLLDGGDYFHGSAVASLTEGEALIPLLNDFNYDLILPGNWEVVYKKKKMLYDMGHATA
                     AKICANMWHKTNDADNGELIYPPYWVKYIAGAKVGFIGYTDHLIPKRQSPAYSEGIRF
                     EHADFNVAKYIRLLKEVEGCGIIFIVTHMGLAQQVGLANNPAVTGADFILGADTHERV
                     RVPIEGKYTKVVECGAFGSFLGKLDIEVENGKMKSYKYQLLDVDPDKYPADKKLQVQI
                     DKATAPFRQQLSKVIGTTTTPLVRYFVMETPMDNLITDAIHWKFKTDIALSNGFRFCQ
                     PLAVDPKTGKAEITKEFLWNMLPVDSEAKTGTVTGRQVWDWLEKELQNAFAKDPSKRF
                     GGWFVRFAGMEVNFTIGNEFGKRVNWVKIKGEQLNLDKEYSIVACEREGDPDDTLCRM
                     EKVKNPKRLGVLMHTVIEEYLGVHSPVSPKLEMRATATDQPHTLLTQLQGTTYEFR"
     gene            complement(3016..>4242)
                     /locus_tag="PK977_07075"
     CDS             complement(3016..>4242)
                     /locus_tag="PK977_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HRF17911.1"
                     /translation="NKEGLTDYYANAVGGGIKFETGRFKNFQVGVSGFFVYNIGSSDL
                     SAPDSKTKQMNRYETGLFDIEDPSNKKDIDRLEELYIKYHFSNNHITLGKQLINTPFI
                     NLQDGRMRPTEVEGIWTEINSIKKLKLQLGYLYGISPRSTVKWFKVAESIGVYPVGVN
                     DEGIKSAYAGNLESKGIFLASITTQINEQLKLQFWNQYTENIFNSAMLQADYEYSLSK
                     NSKLFAAAQLVRQDAVKDGGNDDPSKAYFEKGAKAITFGTRLGWKNEKWETSLNYNRI
                     TAQGRYLMPREWGRDPFFTFLPRERNEGLGDAHAVLGKVNYRIPKARVTTSISFGHYE
                     LPDVTDFRFNKYGMPSYNQLNIDARYKFGGILKGLETQLLYVYKGKTGSSYGNDKYVI
                     NKVDMSLWNIVFNYQF"
     assembly_gap    4243..4262
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4263..4450)
                     /locus_tag="PK977_07080"
     CDS             complement(<4263..4450)
                     /locus_tag="PK977_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17912.1"
                     /translation="MKKNFILIIATVISLSGFTQHQEINEKPGLWKEKENEADSTSIL
                     YAFRKGTFSGHFRYYFMAT"
     gene            complement(4504..4935)
                     /locus_tag="PK977_07085"
     CDS             complement(4504..4935)
                     /locus_tag="PK977_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRF17913.1"
                     /translation="MYKKIIMVISISLLTFQGWSQNTIGPVLQHRIVMQLSSNDTLVW
                     KGLMNNLKHLKAGWGDSVVIEVVAHGPGLDFLTKAKTTQQEKINHFKQLGIMFIACEN
                     TMMERKIPKESIIAEATFVRMGIGEIVRKQEQGWSYIKAGF"
     gene            complement(4955..5374)
                     /locus_tag="PK977_07090"
     CDS             complement(4955..5374)
                     /locus_tag="PK977_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF17914.1"
                     /translation="MSTILKEIKSNSWIFIAAAIMLAGLCGSVCVQAQDNLAGKTISQ
                     KKFERLSKKKNAVILDVRTTDEYKAGHIPHATQIDVLKKEEFKAQISSLDKNKTYLVY
                     CRSGKRSKDAMNIMKELGFTKLFDLNGGFSNWTGAKE"
     assembly_gap    5414..5461
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5462..5693)
                     /locus_tag="PK977_07095"
     CDS             complement(<5462..5693)
                     /locus_tag="PK977_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF17915.1"
                     /translation="MLGFIKKLLGGSSVNYKELVGNGAIIIDVRSAGEYKSGHIPGSK
                     NYPLDSIRTKIAELKKLNKPVITVCRSGARSGM"
     assembly_gap    5831..5888
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6046..>6302)
                     /locus_tag="PK977_07100"
     CDS             complement(6046..>6302)
                     /locus_tag="PK977_07100"
                     /inference="COORDINATES: protein motif:HMM:NF021902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRF17916.1"
                     /translation="FIDPKGTIRLIMYYPLNVGRNMHEIKRVLLALQTSDANKVAIPL
                     NWEPGDKVIVPPPKTLADYEARLADDTVEKIDWYLAKKTL"
     assembly_gap    6303..6328
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6329..6706)
                     /locus_tag="PK977_07105"
     CDS             complement(<6329..6706)
                     /locus_tag="PK977_07105"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRF17917.1"
                     /translation="MEPNTITMPRIGDKAPDFTAVTTKGVIKFSDYAKDNWVILFSHP
                     ADFTPVCTTEMSGFAAEQESFFDKHNTKLMGLSIDSVHSHIAWVDNVKKNTGVYFQFP
                     IIADLDMKVAKLYGMLQPNESETA"
     gene            complement(6789..8207)
                     /locus_tag="PK977_07110"
     CDS             complement(6789..8207)
                     /locus_tag="PK977_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF17918.1"
                     /translation="MKVEQIYTGCLAQGAYYIESNGEAVVIDPLREVEPYIQKAERNN
                     AKIKYVFETHFHADFVSGHLDLSNKTGAPIVYGPNAKPAFDALIAEDGQEFKVGNVTL
                     KVLHTPGHTMESTCYLLKDENGKDVGLFSGDTLFIGDVGRPDLAQKVAADLTQDKLAG
                     YLFDSLRNKIMPLADDITVYPAHGAGSACGKNMSKETTDTLGNQKKTNYALRANMTKE
                     EFIKEVTTGLVAPPAYFPLNVMMNIQGYDSIDKVLERGQHALSPDGFEAAANETGALI
                     LDTRDPQTFAQGFVPNSINIGIDGQFAPWVGAMIPDIKQEILLVTDEGREEEVVTRLA
                     RVGYDYTIGYLKGGFNAWKNAGKEVDTIKSVTADELADISQKEKINILDVRKKSEYLS
                     EHLLEAENAPLDFINDSMAQIDKKKTYYVHCAGGYRSMIFNSILRARGFDNLIDVKSG
                     FKALKESNKFKISDYVCPSTLL"
     gene            complement(8283..9092)
                     /locus_tag="PK977_07115"
     CDS             complement(8283..9092)
                     /locus_tag="PK977_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF17919.1"
                     /translation="MEIIGYIASLLIGVSLGLIGGGGSILTVPVLVYLFGVNPVLATA
                     YSLFIVGATSLVGTYPKYKSGEVNLKTALIFGIPSIIAVFATRAFIVPAIPKEVFQIG
                     DFVVTKSLLMMILFAILMVFASVSMIRNKKQKTAESGVQKFNYPMILLEGAVVGMLTG
                     LVGAGGGFLIIPALVLLSKLPMKQAVGTSLLIIAAKSLIGFTGDLGKQSMDWTLLLSV
                     TGLAIVGIFIGNALSKKVSADGLKKGFGWFVLVMGIYIIVKELFFPGGGGH"
     gene            complement(9162..9779)
                     /locus_tag="PK977_07120"
     CDS             complement(9162..9779)
                     /locus_tag="PK977_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF17920.1"
                     /translation="MNWKEIFPQFEPGLVELIEKEAVQRTFLAGEIIMRTGQYIKSTA
                     LVLEGRVKIYRENPDGGEFLMYYLGPGQACAVTMICAIQSQQSEIMAKAEEDTEVLMI
                     PIQLMDDMMNKYKSWYQFVIMTYRNRFDELLAVIDNVAFRNMDERLEFYLKRHADASG
                     KKLIETSHQQIADDLNSSREVISRLLKKMEQRNLLKLHRNMIELL"
     gene            complement(9790..>10768)
                     /gene="murF"
                     /locus_tag="PK977_07125"
     CDS             complement(9790..>10768)
                     /gene="murF"
                     /locus_tag="PK977_07125"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRF17921.1"
                     /translation="TTTKELVHAVLSSTFKTYTTEGNLNNHIGIPLTILKIKKNAELA
                     VIEMGANHQKEIAGYCLYAKPTHGLITNCGKAHLEGFGGVEGVKKGKGELYDYLRENK
                     GTAFVMWDYDYLQEMSKGIKHIIKYGTTNADIEGSSIKSEPFLVVNITKGSACGTIHT
                     NLVGDYNLPNVLTAVTAGKYFGVKDSVIKMAIENYSPSNSRSQLLEKGTNKIILDAYN
                     ANPSSMRLAIENLAKIDTENKVLILGAMAELGNESLQEHKDIVDLIKKHDWKAVVLVG
                     GDFLRFDHPFSTFANSGEVKGWLKEQNFEHTHMLIKGSRSMQMEKVLDN"
     assembly_gap    10769..10887
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10888..11087)
                     /locus_tag="PK977_07130"
     CDS             complement(<10888..11087)
                     /locus_tag="PK977_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRF17922.1"
                     /translation="MLIEELYQLFEQYPSVQTDTRKLNPGDIFFALKGDNFNGNSFAK
                     KAIEVGAAYAVIDEKEFEIPGKT"
     gene            complement(11161..>11503)
                     /locus_tag="PK977_07135"
     CDS             complement(11161..>11503)
                     /locus_tag="PK977_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HRF17923.1"
                     /translation="STGRVKMVTVTDEEAKKRRNYQRGDVINYLDGDSLSQASYGYAI
                     TTLVSDKSKVYKGGSWNDRAYWLTPGARRFLEEDQALSTLGFRAMNRMGSPEGNKRKT
                     GQHFKTRRQKR"
BASE COUNT         2911 a         2515 c         2106 g         3700 t
ORIGIN      
        1 agcggcgcat acatgcctca atgaggacga aataccaaag gatttgttac ctattatcag
       61 cagtacgggc accatcagtc ctataagcgg accggctaca taccagggcc agggttgttt
      121 cagaaagtct aacatactct tttttgtatt taaaggtcaa aattgatttt taatgaaagt
      181 cttacgagtg atttatgtca caggttaagt tatttctttt ttgccagttc ttcttttttg
      241 gcaatgatag gtttataagg cccatattta tgctggctct cagaaaatcc atccgcatag
      301 gggccaaacg gatgatcaaa aggctttttg gaaatatccg gccagtcatt ccttaaatct
      361 tttttaggcc tgggctgact atttacataa gctgctacat cccatgcctc ttctgtcgta
      421 agttttggat tatcatgtgt ggcttgttta aaaggcatgt tgctttttac ataaccagca
      481 aaattgctca ggcgaaaaag acctgcacca tcattgtaac tatgttctcc ccatagtgga
      541 ggatattcat agctaacgcc attcaaacta agctgccctt ctccgtttgc tccgtgacaa
      601 ctctggcact gtgcggtgta cactgccttg ccttttaccg gatcagcagc cctgtcaaga
      661 tagtttaatt tagaaatacc gcttccaaca ggtcgttctc ctttcttaac atccttgccc
      721 aaccatttga tataagcata aatggcctgg tactccctgc tgttgctatc cggagcagta
      781 ccgttcaggc ttcgttcaaa acaatcacct actcttttgt aaatactttc cataccacca
      841 ctccggtccc ggaacttggg ataggtagaa taaacagcgc tgtaattgtt tccccatgtt
      901 tttttgccgg cattcaggtg gcagttctgg caattcattc cattggtaat agcagcaaca
      961 gagccatgag gtccaaaata ccgggctgta tttgcaatga tatcttcacc ataaatgact
     1021 agttcccttt ctgctccttg taattcatta tctgtaaaca gactgggagt cacccagctg
     1081 ctgtctgtgg ttgtagttag aggagattct ttttcggatg gccttattac ttttgccatt
     1141 tcgactaaag caactattcc aactacagcg gcaataataa ctaactgcca gtttgatctg
     1201 atggtgttcc acataaattt tagcttatct gaattcgtaa gttgttccct gtaattgtgt
     1261 aagcagtgta tgtggttggt cggttgcggt agctctcatt tccagttttg gtgaaaccgg
     1321 tgagtgtact cccaagtatt cctcaataac tgtgtgcatt aatacaccca gtctttttgg
     1381 attttttact ttttccatac ggcaaagtgt atcgtcagga tcaccttctc tttcacaggc
     1441 aacgatgctg tattctttat caaggttcag ctgttcacct tttatcttca cccagtttac
     1501 tctttttcca aactcattac cgatagtaaa attcacttcc atacctgcga atcgcacaaa
     1561 ccagccacca aaacgtttgg atggatcttt ggcaaatgcg ttttgcaatt ctttttccag
     1621 ccagtcccat acctgcctgc cggtaactgt tccagtcttt gcttcgctgt caacgggcag
     1681 catgttccat aaaaactcct tggtgatttc agctttcccg gtttttggat ctactgctaa
     1741 tggctgacaa aagcgaaagc cattacttag tgcaatatct gttttgaatt tccaatgaat
     1801 agcatcagta atcaggttat ccattggggt ttccatcaca aaatatcgta ccagtggggt
     1861 agtggtggtt ccaattactt tagaaagttg ttgtctgaaa ggcgcagttg ctttatctat
     1921 ctgtacttgt aattttttat cagccgggta tttgtcaggg tctacatcga gcaattggta
     1981 tttgtaactc ttcattttcc cattctccac ttcaatatcc agtttgccaa gaaaagaacc
     2041 aaaagcgccg cattcgacta ctttggtgta tttgccttca ataggaaccc ttactctttc
     2101 atgggtatct gcacccaaaa tgaaatctgc gcctgtcact gctgggttat ttgccagtcc
     2161 tacttgttga gccaggccca tatgggtaac aatgaaaata atgccgcagc cttctacttc
     2221 ttttagtagc ctgatgtatt tagccacatt aaaatcagca tgttcaaatc gaattccttc
     2281 gctgtaagcc ggagactggc gcttaggtat caggtgatcg gtatagccaa tgaaaccaac
     2341 tttggcaccg gcaatatact taacccaata gggtgggtag atcaattctc cattatctgc
     2401 atcgttagtt ttatgccaca tattggcaca aatctttgct gctgtagcat gccccatatc
     2461 atagagcatt ttctttttct tatagaccac ttcccagttg cccggcagaa taagatcgta
     2521 attgaaatcg ttgagcagag gtatcagtgc ttcgccttct gtcagtgatg ctacggcact
     2581 tccatggaaa tagtcccctc cgtccagcaa caaggtattt gccgggtttt gtttacgaaa
     2641 gtgattgatc atggttttta atacggctaa ccctcctctc tttctgtaca ctgctttccc
     2701 gttttcccag aaaaattcat catgagtgtg caactgcgca tgaatgtcgg aagtataaag
     2761 tattgtaaat cggccgggtg tgccaggctc agatttttta ctcagctcgg ttgtgccggc
     2821 tatagctaca gatcccaggg ttgtacccat accagccagt gcagcttttt tcaaaaaatc
     2881 ccggcgggaa ttagtatcca tgtttttcaa tgaagacatc ggcaatcgtt ttaggtgaaa
     2941 aattacggga attaaaaatg ctttctttaa gtaacccggt ccgtgacaac tgtcacattc
     3001 ccctttttat atttcttaaa actggtaatt gaatacaatg ttccaaagac tcatatccac
     3061 tttattgata acgtatttgt catttccgta actactgcct gtttttcctt tataaacata
     3121 tagtagctgc gtttccagac cttttaatat gccaccaaat ttatagcggg catcaatatt
     3181 caactggtta tacgagggca tcccatattt attgaacctg aaatctgtta catccggcaa
     3241 ctcataatgc ccgaaggata ttgaagttgt gaccctggcc ttcggtattc tgtaattgac
     3301 cttccccaac acagcatgtg catctcccag cccttcattt ctttctcttg gcaaaaaagt
     3361 gaagaaagga tcccggcccc attctctcgg cataaggtat cttccttgag cagtaatacg
     3421 gttatagttc aggcttgttt cccatttttc attcttccat cccaacctgg ttccaaaagt
     3481 tattgctttg gcgccctttt caaaataagc ttttgaagga tcatcattgc caccatcctt
     3541 tacagcatcc tgccttacca actgtgccgc tgcaaagagc tttgaatttt tggatagcga
     3601 atattcatag tctgcctgca gcatagcact gttaaagata ttttctgtgt attggttcca
     3661 aaactggagt tttaactgct catttatttg ggtagtaatg ctggccagaa aaattccttt
     3721 gctttccagg tttccggcat aagctgattt aataccctca tcatttaccc ccacaggata
     3781 taccccgatt gattctgcta ctttaaacca cttaactgta cttctgggcg aaatgccata
     3841 taagtatcca agctgcaact tgagtttttt tatggagttt atttctgtcc agatcccctc
     3901 cacttctgtt ggcctcatcc ggccatcctg caggttaata aatggggtat tgatcagttg
     3961 tttacctaag gtaatatgat tgttactaaa atgatacttg atatacaatt cttctaagcg
     4021 gtcaatgtct tttttgttag acgggtcttc aatgtcaaac agtccggttt cataccggtt
     4081 catttgtttg gttttggaat caggtgctga aagatcggag gagccaatat tataaacaaa
     4141 aaatccgctc acccccacct gaaaattttt aaacctgcct gtttcaaatt ttattcctcc
     4201 tcctactgcg ttggcataat aatctgtcag tccctctttg ttnnnnnnnn nnnnnnnnnn
     4261 nngttgccat aaaataatac ctgaaatgac cactgaaagt acccttcctg aatgcatata
     4321 aaatcgaagt ggaatcagct tcgttctctt tttccttcca caagcccggt ttctcattga
     4381 tctcctggtg ctgtgtaaat cctgaaaggg aaatcactgt tgcaatgatg agtatgaaat
     4441 tctttttcat tatgctataa gcattatgtt tttgactaat tggcaaacag tctgtttgtg
     4501 gaatcagaaa ccggctttta tataactcca gccttgttct tgttttcgta cgatttcgcc
     4561 gattcccatt cttacaaaag tggcttcggc tataatggat tctttaggta tttttctctc
     4621 catcatggta ttttcgcagg caatgaacat aatacccaat tgtttgaaat ggtttatttt
     4681 ttcctgttga gttgttttgg cttttgtcag aaaatcaagg cccgggccat gcgccacaac
     4741 ttcaataaca acgctgtcac cccatccggc tttaaggtgc ttcaggttat tcatcagacc
     4801 tttccaaacc aatgtatcat tgctgcttag ctgcataacg atgcggtgct gtaaaaccgg
     4861 tccgattgta ttttgagacc atccctgaaa tgttagcaat gatatagaga tgaccatgat
     4921 gattttttta tacataacct tacagtttta gttgttattc ttttgcccca gtccagttgg
     4981 agaaacctcc gttcaggtca aagagcttgg taaatcctaa ttctttcatg atgttcatgg
     5041 catccttact tctttttcca ctgcggcaat agaccaggta tgttttgttc ttgtccaggc
     5101 tggaaatctg tgccttgaac tcttcttttt ttaacacatc aatttgtgta gcatgtggta
     5161 tatggcctgc tttatattca tcagttgtcc ttacatccag tattactgcg ttctttttct
     5221 tgctgagccg ttcaaatttt ttctgactta ttgttttacc tgccagatta tcctgagctt
     5281 gtacgcaaac agagccacat aggccagcca gcattatggc tgcagcaata aatatccagc
     5341 tgttgctttt gatttctttt agtatagtgc tcatgctatt ttatttttca gactgttcca
     5401 gggccctccg ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nccattccgc tacgggcacc acttctgcat acggtgatca ccggcttatt cagtttttta
     5521 agttcagcta ttttagtacg tatgctatca agcggatagt tctttgaacc gggaatatgc
     5581 cctgatttgt actcaccagc agaccgaaca tctataatga tagcgccatt gccaacaagc
     5641 tctttgtagt taacagaact tccgcccaat agcttcttta taaatcccaa catagtttta
     5701 aattagttta ataaaaattg catatctcct aaaccaccgc cgttatatac ttcagtaata
     5761 ccattttgtt tcagattgct tactgccatt ccgctcctgt tgccgctgcg gcaatagagc
     5821 actataggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnngg aatattcttg gctcccggta catgttccat gtcaaattcc cacggacttc
     5941 ttacatcgac aattactgtt tcaggttttt ttactatctc ttttaatgac tgcattgctt
     6001 ttttagtttt aaaaaagcgg ctgttgttat ggcagccgct tggttttata atgttttttt
     6061 ggctaagtac cagtctatct tttctaccgt gtcgtcagct agccttgctt cgtaatcagc
     6121 caacgtttta ggtggcggta cgattacttt gtcacccggt tcccagttaa gagggattgc
     6181 cactttgtta gcatcgcttg tttgcaatgc aagcagcact ctttttattt catgcatatt
     6241 cctgccaaca ttcagcgggt aatacatgat aaggcgaatc gttcccttcg ggtctatgaa
     6301 aannnnnnnn nnnnnnnnnn nnnnnnnnag cagtttcact ttcattgggt tgcagcatgc
     6361 catataattt ggctactttc atatccagat cagcaatgat gggaaattgg aaatacacac
     6421 cggtgttttt cttcacatta tcaacccagg caatatgtga atgaacactg tcgatactta
     6481 atcccattaa tttggtatta tgcttatcaa agaagctctc ctgttctgcc gcaaagccac
     6541 tcatttctgt agtgcacaca ggggtgaaat cagcagggtg ggagaataga attacccagt
     6601 tgtctttcgc atagtctgag aatttgatca ctcccttggt ggtcacagct gtaaagtcgg
     6661 gtgctttatc accgattctt ggcattgtta tcgtattggg ttccatgttt tgatttttaa
     6721 ttgttttttt aataaggccg gggaagattc cccggctctt ttaccttaac cattaaacca
     6781 accaacattt ataatagtgt cgaaggacac acataatcgc ttattttgaa cttgttgctt
     6841 tctttaaggg ctttgaaacc actctttaca tcgatcagat tgtcaaatcc cctggcacgg
     6901 agaattgagt tgaatatcat agagcggtaa ccaccggcac aatgaacata gtatgtcttt
     6961 ttcttatcga tctgggccat gctgtcgttg ataaaatcaa gtggtgcatt ttctgcttct
     7021 aacaggtgtt cgctcaggta ttcacttttt ttacggacgt caagaatgtt tatcttttct
     7081 ttctgtgata tgtccgcaag ttcatctgca gttacagatt taatcgtatc aacttccttt
     7141 ccggcatttt tccaggcatt gaaaccacct ttcaggtaac caatggtata atcataacca
     7201 accctggcca atcttgttac cacttcttct tcacgacctt catcggttac cagcaatatt
     7261 tcctgtttga tatcgggaat catggcgcca acccatgggg caaactggcc atcaatacca
     7321 atatttatag agttaggaac aaatccctgc gcaaaagttt gcggatctct tgtatccagt
     7381 attaatgcac cggtttcatt ggcagcagcc tcgaaaccat cagggcttaa tgcatgctga
     7441 cctctttcaa gaactttatc aatactgtca taaccttgta tattcatcat cacattcaat
     7501 gggaaatagg caggaggagc aacaagtccg gttgttactt ctttaatgaa ctcttccttc
     7561 gtcatatttg ccctgagagc atagtttgtt tttttctgat tacccagtgt atctgtggtt
     7621 tccttactca tgtttttacc acaggcgctg ccggcaccgt gagcaggata tacagtaata
     7681 tcatcagcca gtggcatgat cttatttcgg agagagtcaa ataaataacc agctaattta
     7741 tcttgtgtaa gatcagctgc aactttttgg gcaaggtcag gacgacctac atcaccaata
     7801 aacaatgtat caccagagaa caaaccaaca tccttgccat tttcatcttt cagcaggtag
     7861 caggtgcttt ccattgtatg accaggagta tgcagcactt tcaatgttac attacctact
     7921 ttaaattcct gcccgtcttc agcaatcaag gcatcaaagg caggttttgc attggggccg
     7981 tacacaatgg gtgcgccggt tttattgcta aggtcaaggt gaccgctgac aaagtcggca
     8041 tggaagtgag tttcaaaaac atacttgatc tttgcattgt tacgttcagc cttttgaata
     8101 taaggctcta cctcacgcag cggatcaatt acaactgctt caccattact ttcgatgtag
     8161 taagcacctt gtgccaagca accggtataa atttgttcta ctttcatttg ctatattttt
     8221 tgtttttatt gaagatcaaa tttcggatca ggaaacatgc tgaacagtga catcaatcac
     8281 aatcaatgac caccgccacc agggaagaac aactctttaa caattatata aatacccatt
     8341 acgagtacaa accagccgaa cccttttttc aggccatcag cagatacttt tttactgagt
     8401 gcattaccga tgaaaatccc aacaatggca aggccggtaa cggaaagtaa aagtgtccag
     8461 tccatgcttt gtttgccaag atcaccggta aaaccgatta atgattttgc agctatgatt
     8521 aatagtgaag ttccaactgc ctgtttcatg ggcagtttac ttaatagtac caatgccgga
     8581 ataataagaa aaccgcctcc ggcgcctact aaacctgtaa gcatgcctac aacagcccct
     8641 tccagcagaa tcatcgggta attgaatttt tgcacaccgc tttctgctgt tttctgtttt
     8701 ttattcctga tcattgagac agaggcaaat accatcagaa tggcaaatag tatcatcatc
     8761 agcagcgatt ttgtcactac gaaatcaccg atttgaaata cttctttggg aatagccggt
     8821 acaatgaagg cccttgtagc aaatacagct ataattgaag gaattccgaa gataagagct
     8881 gttttcaggt tcacttcacc acttttatat tttgggtaag tacctaccag gctggtagct
     8941 cctacaataa aaagtgaata agctgtagca agtacgggat ttaccccaaa aagataaacg
     9001 agaacaggta ctgtgagaat ggagccgcct ccgccaatta agccaagaga aacaccgatc
     9061 agaagagaag ctatgtatcc gattatttcc atgcaaaaat catttatgca aaggtgaacg
     9121 gaatacaaat gctgtactgt gactcaaatc acagcatctt gctatagtaa ttcgatcata
     9181 tttctgtgga gtttcagcag gttgcgctgc tccatttttt tcaggagtct tgaaattact
     9241 tctcttgaac tattaaggtc atcggctatc tgctggtgag aagtttcaat caattttttc
     9301 ccgctagcat cagcatgtct tttcagatag aattcaagcc tctcatccat atttcgaaag
     9361 gccacattgt caatcacggc taacaattca tcaaaacggt ttctataggt catgatgaca
     9421 aactggtacc acgatttgta tttgttcatc atatcatcca ttaactggat gggtatcata
     9481 agtacttcag tgtcttcttc tgcttttgcc ataatctcac tttgttgtga ctgtatggca
     9541 cagatcattg taacagcaca ggcctggccc ggccctaaat aatacatcag gaattctccg
     9601 ccgtcagggt tttccctgta aatctttacc cttccttcca ataccaatgc agtggattta
     9661 atgtattggc ccgtacgcat tattatttca cccgcaagga aagttctttg caccgcttct
     9721 ttttcaatta actcaaccaa tccgggttca aactggggaa atatttcttt ccaattcatg
     9781 atccttgtat taattatcta aaaccttctc catttgcata cttcggctac ctttaataag
     9841 catatgtgtg tgttcaaagt tttgctcttt cagccagcct tttacttcgc ctgagttagc
     9901 gaaagtactg aaagggtggt caaatctcag aaaatcaccg cctactaata caacggcctt
     9961 ccagtcatgt tttttgatca gatcaacgat gtctttatgt tcctggaggc tctcgttacc
    10021 taattctgcc atggcaccaa gtataagcac cttgttttcc gtatcaattt tagcaagatt
    10081 ttcaatagca agcctcatgc tgcttgggtt ggcattatat gcatccagta tgattttatt
    10141 agttcctttt tcaagtagct gagatctgct attggagggg ctataatttt caattgccat
    10201 ttttataact gaatctttta ctccaaaata ttttccggct gttacagctg tcaacacgtt
    10261 gggcagatta tagtccccca cgagatttgt gtgaatagta ccacatgccg agcccttagt
    10321 aatatttact acaagaaacg gctcactctt gatcgatgat ccttcaatgt ctgcattggt
    10381 cgttccatat tttattatat gctttattcc tttgctcatt tcctgcaggt agtcataatc
    10441 ccacatcaca aaggcagtac ctttattctc tcttaaataa tcatatagtt cacctttacc
    10501 cttttttaca ccttccacac cgccaaaacc ttccaggtgg gctttgccgc agttggtaat
    10561 aagcccatgg gtgggttttg cataaaggca atagccggct atttcctttt gatggttggc
    10621 ccccatctca attactgcca gctcagcatt ttttttaatt tttaatatag tcagcgggat
    10681 gcctatatga ttgtttaggt tgccttctgt agtgtaagtt ttaaatgtgg aggaaagcac
    10741 agcatgaacc agttctttgg tggtggttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnngtt ttacccggaa tttcaaattc tttttcgtca
    10921 ataacagcat aagcagctcc cacctcaatg gctttttttg caaaactgtt tccattgaaa
    10981 ttatctcctt tcaatgcaaa gaaaatatcc ccggggttga gtttccgggt atccgtttgt
    11041 acagaagggt attgctcgaa taattggtaa agttcttcaa ttagcatata gcaaaagtag
    11101 gagagtagtc aataaaaaac cctccaggtt ttggagggtt tgagaaagaa ttattcttaa
    11161 ttatcttttt tgtcttcttg ttttgaagtg ctgaccggtt ttacgtttgt taccttccgg
    11221 acttcccata cggttcatag cacggaaacc gagggtgctg agtgcctggt cttcttccag
    11281 gaaacgtctt gcgcctggag taagccagta agccctgtca ttccagctac cgcctttata
    11341 aacttttgac ttgtcgctta ccagtgtagt aattgcataa ccatatgaag cctgagagag
    11401 agagtcacca tccaggtaat ttattacatc acctctttgg tagtttcttc ttttcttcgc
    11461 ttcttcatca gttacggtta ccattttaac acggccggta ctg
//
LOCUS       DAORLS010000070        11454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3021_length_11454_cov_2.089218, whole genome shotgun sequence.
ACCESSION   DAORLS010000070 DAORLS010000000
VERSION     DAORLS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11454
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3021_length_11454_cov_2.089218"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK977_07140"
     CDS             <1..421
                     /locus_tag="PK977_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF17924.1"
                     /translation="GKANIVTNADAGKIANGESCVIITYGMGVYWAKAAAKQFNGQVE
                     VVDLRTLFPLDEELIYERVKAHGKCLVLTEEQQNNSFAEALAGRISKNCFQWLDAPVE
                     VLGALNLPAVPMNMGLEKAMLPDAEKVIKVLKLLLSA"
     gene            complement(487..2316)
                     /gene="aspS"
                     /locus_tag="PK977_07145"
     CDS             complement(487..2316)
                     /gene="aspS"
                     /locus_tag="PK977_07145"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRF17925.1"
                     /translation="MYRTHTCGELRLTNKGTEVTLAGWVQTVRKFGAITFIDLRDRYG
                     ITQLLFGEELNKQLEENPLGREFVLQATGTVNERSNKNPNIATGDIEILVSSFKVLNK
                     SAVPPFTIQDDTDGGDDLRMKYRFLDLRRNAVKKNLELRYAVNRAARNYLHQNNFMDI
                     ETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMVSGYDRYYQVVKCFRD
                     EDLRADRQPEFTQIDCEMAFVEQEDILNMFEGMIKSIFKEVKGIDYTESVPRMTWEDA
                     MWNYGNDKPDIRFGMQLLNLKKPATVYVDKQDSSPLINGADFKVFDEAETVLAIAVPG
                     CSEYSRKQTDELTEWVKRPQIGMKGLVFIKCNADGTYKSSVDKFYSEERLKAIADAAN
                     AKAGDLVLILAGTEERTRKAISELRLEMAERLGLRKKDEFKLVWVLDFPLFEYDEEGN
                     RWVARHHPFTSPKPSQIEMMINNNPVIENAAEYLKHPYANIKANAYDMVLNGNEIGGG
                     SIRIFQRELQEKMFAALGMDAAEQQHKFGFLLGAFEYGAPPHGGIAFGFDRLCAILGG
                     SESIRDFIAFPKNNAGRDVMLDAPATIDEKQFEELQIKLNLKG"
     gene            2617..3507
                     /locus_tag="PK977_07150"
     CDS             2617..3507
                     /locus_tag="PK977_07150"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRF17926.1"
                     /translation="MLANRIEFGYEKAEEHHVDLSWLLVIVVLAGIAIIAGLLISIIK
                     HFLSTSKKKGETIAVNATEENATVYDSWLSMYNPYYATLSPELKSRFIYRVIQFIQSK
                     KFSFHSVEPDEQAVVLISGAAIQISFGLKNFLLDHYPVINVIKKEYVLKADNDTYYGH
                     VSRNGIYVSWKHFQEGYEDYSDSVNVGLHEMAHALSFDAYLGNEDIHDRKFKTRLQNF
                     VKDGVPVFRALKSTSSHVLDDYASTNFDEFWAVSVETFFENTESFRQNRPELYQEMCE
                     TLNQDPLSPSKIIDSHIAGF"
     gene            complement(3526..3936)
                     /locus_tag="PK977_07155"
     CDS             complement(3526..3936)
                     /locus_tag="PK977_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17927.1"
                     /translation="MEQTNEAIYSIPSRYRRMENMHIVFWLVKDISWCLIWRELGLAM
                     FIPTLVISIIIAWRTRQMKSELAHNLAITFWICANGYWMISEFFGFDEVIVWNQFTGK
                     HMALIPFFTGAAILLYYYTIQRPREIKEHRATTM"
     assembly_gap    4046..4055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4056..5035)
                     /locus_tag="PK977_07160"
     CDS             complement(<4056..5035)
                     /locus_tag="PK977_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF17928.1"
                     /translation="MQFQYLIIGQGISGTWLSYYLEKSDRSFLVIDNNDPNAPSRLAA
                     GIINPVTGRRHVTAWMIEELLPFAWNAYTEFGKQLGIKAISQKDIIDFFPSAQMRESF
                     QQRVNENAAYVSLDESVNYNDLFNYELGYGQVKPAYTAHLDNIIPAWRKILTYKGKIL
                     NEQFDLSQLIFTTEVVRYKEITAEKIIFCDGAIAGSNPYFNTLPFAPNKGEALTLEIP
                     DLPAERIYKRGMMLVPLATAGHWWIGSSYQWDFDDLNPTLAFREKTELLLREWLKVPY
                     TVTGHIASNRPATLERRPFVGLHPLHSQVGILNGMGTKGCSLAPFFAKQLA"
     gene            complement(5061..5603)
                     /locus_tag="PK977_07165"
     CDS             complement(5061..5603)
                     /locus_tag="PK977_07165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRF17929.1"
                     /translation="MTRLIIARHGETIENGLNICQGQKHGTLSEKGIKENENLGVQLK
                     QFSFQTIYSSPLNRALQTAQAIHKHNNSSRIITDERLMERNLGVLQGKVYPQPYFETD
                     IYEDMETIESVAERSAAFLDEIRQNHNNETIVLVSHGYIIKVMLALLRNLPLAEFSKI
                     KLMNNSSYTTESLNQIMQKG"
     gene            complement(5619..>6863)
                     /locus_tag="PK977_07170"
     CDS             complement(5619..>6863)
                     /locus_tag="PK977_07170"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRF17930.1"
                     /translation="IAPGQEGYLKAILTKAIPYLDRQIKEDYDNLVKYKTNLKTYTPG
                     YTTIQYLYMRSFFPDYEIATASKKAYDYFRSRMQQTWVGQSKYMQGMAALALYRTADT
                     KTPSAILNSLKETSISNEELGMYWKDQRNGWFWYEAPIETHSLLIETFHEIGKDNKTI
                     DDLKTWLLKNKQTNNWKTTKATAEACYALLLQGNDWLINDPIVEIKLGPAFIKTTDEK
                     EAGTGYIKKTIEGRLVKPEMGNISVSVSSANNQSPVTWGAVYWQYFEDLDKITTASTP
                     LKLNKKLFVEKNTDRGPVITPVNDGDAIKVGDKIKVRVELRVDRDMEYVHMKDMRASA
                     FEPVNVLSSYKWQGGLGYYESTKDASTNFFFSYLRKGTYVFEYTLFATHTGNFSNGIT
                     SIQCMYAPEFSSHSEGIRVNVE"
     assembly_gap    6864..6873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6874..>9796)
                     /locus_tag="PK977_07175"
     CDS             complement(<6874..>9796)
                     /locus_tag="PK977_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRF17931.1"
                     /translation="YQDLDTIKIKTNEFGSFSGRFQLPESGLNGLFNIYTKPDAGNAS
                     FRVEEYKRPKFYVDYETIKGTYKVNDMIKVTGIAKAYAGNNIDGATVKYRVVRQPRFI
                     YPWLFWRWWQPPSDEMEIAHGEAKTDKDGKFSFEFKAIPDLKVDKKFEPVFDYTVYAD
                     VTDINGETRSGEKSVSVSYKSLMLVANVPSTLPADSLKSLSIRTQNMNGEYEPAIIKV
                     TISKLKEEKRLIRNRFWQRPDQFVMSKEEYIKNFPHDEYDNESDYKSWEKGEQVFSKA
                     DSVNKNGSWPIVHGSWLPGFYMVEISTKDKNGEEVKDVKYIELTDEKSNQLNQPDYLW
                     TGAKKITVEPGEEATVEIGTAADNLFIVHQVNKSMVNSKQSTTDYSFLQLNNQKKTFS
                     FPATEADRGGFGANWLFVKHNRVYQLNQSINVPWTNKDLQIEYMTYREKTLPGSEEKW
                     KIKITGYKKDKVAAEMLASMYDASLDQFYSHNWNEPSIWPYYYNNKTWSGSQNFSQLQ
                     SYQKYVYAEESKYVTKAYDYLFQSDIEEYGNQTYYYRTRNVSEEASGAPAPAVADTSA
                     VADTNDPSKIVAYKVTMRDKDGDGVTDRIDDKKNNQQDASIKIRKNFNETAFFLPELR
                     TDSSGAIEFSFTMPEALTKWKFMALAHTKEAAFGSSTKEIITQKELMVQPNAPRFLRE
                     GDKMEFSTKVVNLTDKEMTGTAELQLFDATTGEPVDGWFHNSVANQYFTVAAGQSEAL
                     KFPIDIPYQFNKALVWRIIAKSGSYSDGEENALPVLTNRMLVTESMPLPMRGTGTKNF
                     SFEKLLNSGKSESIQHHSLTVEYTSNPAWYAVQALPYLMEYPYDCAEQTWNRYYANSL
                     ASFIANSSPRIKQVFEKWKTKDTAALLSNLQKNQELKAILLEETPWVLQAKNENEQKK
                     NIALLFDMIKMAEQLQSSYDKLKQMQSSNGGFVWFKGGPDDRYMTQYIVTGIGHLKKL
                     KA"
     assembly_gap    9797..9877
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9878..>10344)
                     /locus_tag="PK977_07180"
     CDS             complement(<9878..>10344)
                     /locus_tag="PK977_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17932.1"
                     /translation="EYILVASNEKNLDAQKALIGARLFYVSAISYVNNSDDFFVLNRD
                     NGQPLAKAAVQVWEQKYDYNQRKYIKEKGKTYLTDNNGFFRMEKGKRDNTNYGNYAYL
                     LDVTHNSDRLFMNDFVYDYYYYRDPQTEDAKETTSIHLFTDRSIYRPGQTVYF"
     assembly_gap    10345..10354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10355..>11454)
                     /locus_tag="PK977_07185"
     CDS             complement(<10355..>11454)
                     /locus_tag="PK977_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRF17933.1"
                     /translation="IEKEITQSKEPSASILKSLQAAMYWNYYQQNRWKLYNRTKTENF
                     DKKDITTWTAEDFHKKIGELYLQSIKEEKLLQQSRLETFDAIIIKGNVRHLRPTLYDL
                     LAHRALSYFENDERDIKKPAYAFEIDKASAFDPAADFVTGKFPTKDSQSIQHKALLIY
                     QKLIAFHLKDAKPDALIDADLERIEFVKSNSTHPEKDKLYFNAINHIAKQYGDLPAAS
                     QAWYLLAEWHQEKANEYKPYGDTTWRFSRIKAKEICEKILLQKDSSEGKINCHNLLQQ
                     INSSSLQFSIEKVNLPGQPFRSLINYKNISTLYLRLIKADDNLKKLLENQYDEKYWPA
                     IIAATPIRNWQQVLPSTNDLQSHSAEIKIDALP"
BASE COUNT         2881 a         2338 c         2342 g         3782 t
ORIGIN      
        1 gggtaaggca aatattgtaa caaatgctga tgccggtaaa attgccaatg gagagagctg
       61 tgtgattatt acgtatggta tgggtgtgta ctgggctaaa gcagcggcga agcaattcaa
      121 cgggcaggtg gaagtggtag acctgcgtac cttgttcccg ctggatgagg agttaattta
      181 tgaaagggta aaagcacacg gcaaatgctt ggtgctgaca gaagaacagc aaaataattc
      241 ctttgctgaa gcattggcgg gacgtatctc caaaaattgc tttcaatggc tggatgcacc
      301 ggtagaagtg ctgggtgctt tgaacctgcc ggctgttccc atgaatatgg gattggaaaa
      361 agcaatgctg ccggatgcgg agaaggttat taaagtattg aaactgcttc ttagtgcatg
      421 accaatagcc gatattttga ggtgatgaaa taagcgttaa tccgttagat taatattgcg
      481 gaatacttat ccctttaaat tcagttttat ctgtaactcc tcaaactgct tttcatcaat
      541 cgtggcaggt gcatccagca tcacatcgcg acctgcattg tttttcggga aggcgataaa
      601 atcacggatg ctttcgctgc cgccaaggat tgcgcaaagc ctgtcaaacc caaaagcgat
      661 accaccatgc ggtggtgcac cgtattcaaa agcgccgagt aagaagccaa atttgtgttg
      721 ctgttcagct gcatccatgc ccaaggcagc aaacattttt tcctgcagct ctctttgaaa
      781 aatacggatg gaaccaccgc caatttcatt tccattgagc accatatcat aggcattggc
      841 cttgatgttg gcatatggat gtttcaggta ttcagcagca ttttcaataa cggggttgtt
      901 attgatcatc atttcaatct gtgatggctt gggagaggtg aacggatggt gacgggctac
      961 ccagcgatta ccttcttcat catattcaaa taacgggaag tcaagcaccc atactaattt
     1021 gaattcatct tttttgcgca aacccaaacg ttcagccatt tccagccgca gttcgcttat
     1081 tgcttttctt gtcctttctt cggtgcctgc aagaattaat accaggtcgc cggcctttgc
     1141 attggcggca tcggcaatgg cttttagtct ttcttcactg taaaatttat ctacactgct
     1201 tttgtatgtg ccgtcagcat tgcacttaat aaaaacaagt cccttcatgc cgatctgtgg
     1261 ccgttttacc cattctgtta attcatccgt ttgtttacgg ctgtattcgc tgcaaccggg
     1321 aacggcaatg gccagcactg tttctgcttc gtcaaaaact ttgaaatcgg ccccgttgat
     1381 caggggagaa ctgtcttgtt tgtcaacata tacagtggct ggttttttca ggttcagcaa
     1441 ctgcatgcca aaacggatat cgggtttatc gttgccataa ttccacatgg catcttccca
     1501 ggtcattctt ggaacggatt ctgtataatc aattcctttt acctctttga aaatggactt
     1561 gatcatacct tcaaacatgt tcaaaatatc ttcctgctcc acaaaggcca tttcgcagtc
     1621 tatctgggta aattcaggct ggcggtctgc ccgaaggtct tcatcacgga agcattttac
     1681 aacctggtag taacggtcat aaccacttac catcagcagt tgtttgaagg tttgtggcga
     1741 ttggggcagg gcatagaact gtccggggtt cattcttgac ggtaccacaa aatcccttgc
     1801 accttccggt gtagatttga tcaggaatgg cgtttcaata tccatgaaat tattctggtg
     1861 caggtaattt ctggcggcac ggttcaccgc ataacgcaat tcaagattct tctttacagc
     1921 gtttcttctc aggtcgagaa agcggtattt catccgcagg tcatcaccac catcggtatc
     1981 atcctggatg gtgaagggtg gaactgctga cttgttcaga actttaaaag aagaaacaag
     2041 tatttcaata tcccccgtgg cgatattggg atttttatta cttctctcat tgacagtacc
     2101 ggtggcctgc agtacaaact cacggcccag cggattttct tccaattgtt tatttagttc
     2161 ttctccaaac aataactggg taatgccata ccggtcacgg aggtcaataa atgtgatagc
     2221 cccgaattta cggactgttt gtacccatcc ggccagggtt acttccgttc ctttgtttgt
     2281 cagccttaat tcgccacaag tgtgagttct gtacatgttc agttgaaaag ttgataggtt
     2341 gtaaagttga aatggggcgc taccaataac gaaagccccg gttgccgcaa agataagtta
     2401 gcagcctctt ttttaggcct gaatttgaag agtcagaata tacctgtttt ttgagtgtta
     2461 tcccacagaa ttcgggtttt tattaccata agaataattt tattagttta cctgaaaact
     2521 cttcattgat atttagaaat gccaccacct gatacgattc atataactga cagaattcaa
     2581 attccagact caattaattc tttaattgat tcaattttgc tggcaaatag gattgagttt
     2641 gggtatgaaa aagcggaaga acatcatgta gatttatcct ggcttttggt aattgtagtg
     2701 ttggcaggta ttgctattat agccggactt ctaatatcca ttataaaaca tttcctaagt
     2761 actagtaaga agaagggaga gacaattgca gtcaatgcaa cggaggaaaa tgctactgtt
     2821 tatgatagct ggctcagtat gtataatcca tattatgcaa cactttcccc cgaattaaag
     2881 agccggttta tttatagagt tattcagttt attcaatcaa agaaattttc ttttcattca
     2941 gttgagcctg atgaacaggc tgttgttttg attagtggtg cagccataca aataagtttt
     3001 ggactaaaaa atttcctgct tgaccattac ccggtcataa atgtcattaa gaaggagtat
     3061 gtgctaaaag cggataatga cacttattat ggtcatgtaa gccgcaacgg gatttatgtt
     3121 tcctggaagc attttcagga ggggtacgaa gattattccg attcagtaaa tgttgggctg
     3181 catgaaatgg cacatgccct ctcatttgat gcctaccttg gaaatgaaga tattcacgac
     3241 aggaaattta aaacccggct tcagaatttt gtaaaagacg gggtgcctgt tttcagagca
     3301 ttgaaaagca catcaagtca tgtactggat gattatgctt ccacaaattt tgatgagttt
     3361 tgggcagttt cggtggaaac tttttttgaa aacacggaat cattcaggca aaatcggccc
     3421 gaattatacc aggaaatgtg tgagacatta aatcaggatc ctttgtcgcc aagcaagatt
     3481 attgattctc atattgcagg gttttgattg tatactcata tttacttaca tagttgtagc
     3541 cctatgctct tttatttccc tgggtctttg tatggtataa taatatagta agatagcagc
     3601 cccggtaaag aaaggtatca gcgccatatg ttttccggta aactgattcc acactattac
     3661 ttcatcaaac ccaaaaaatt cactgatcat ccagtagccg ttggcacaaa tccaaaaagt
     3721 aatggccagg ttgtgtgcca gttcagattt catttgtctt gttcgccaag caatgattat
     3781 tgaaatgaca agagtgggaa tgaacatggc taatcccagt tccctccata taaggcacca
     3841 gctgatgtct tttaccagcc aaaaaacaat atgcatattt tccatgcggc gatagcggga
     3901 ggggatgctg taaatagctt cgttggtctg ttccatcttt tgtgagttaa gaaaagtaag
     3961 ttaagaaatg ttgcgggaaa gaatcctggt aaaccgtcta acatccgctt ctgctgttat
     4021 tggcgtattg taaagcaggt gatctnnnnn nnnnngccag ttgtttggca aagaaaggcg
     4081 ctaatgagca gccctttgtt cccataccgt ttaatatgcc aacttgcgaa tgcaatgggt
     4141 gtaaacctac aaagggcctt ctttctaaag tggcgggtct gttactggca atatgtccgg
     4201 taacagtgta aggaactttc aaccattctc ttagcaataa ttctgtcttt tctctgaatg
     4261 ccagtgttgg attcaggtca tcaaaatccc attgataaga agaacctatc caccaatgac
     4321 cggctgtggc cagcggaaca agcatcatcc ctctcttata aatccgttca gccggaagat
     4381 ctgggatttc aagtgtcaat gcctctcctt tgtttggtgc aaagggtaag gtgttgaaat
     4441 agggattgct tccggcaata gcgccatcac agaaaataat tttttcggca gttatttctt
     4501 tataacgaac tacttcagtt gtaaagatca gctgtgaaag atcaaattgc tcatttagta
     4561 ttttcccttt atatgttaaa atctttctcc atgccggtat gatattgtct aagtgggctg
     4621 tgtaagctgg tttcacttgt ccatatccca gttcataatt aaacagatcg ttataattga
     4681 ccgattcatc caatgaaaca taagctgcat tttcgttcac ccgttgctga aagctttccc
     4741 tcatctgggc actgggaaaa aaatcaatga tatctttttg tgaaatagcc tttataccga
     4801 gttgcttgcc aaattctgta taagcattcc aggcaaaggg aagcagttct tcaatcatcc
     4861 atgcagttac atgtcttctg ccggttacgg gatttattat gccggctgca aggcgtgaag
     4921 gcgcatttgg gtcgttatta tctatgacaa gaaatgacct gtcgcttttt tcgagataat
     4981 aactaagcca ggtgccactt atcccttggc cgatgataag atactgaaac tgcatgaggc
     5041 aaaagtaaat gaaatgaggc ttagcctttt tgcataattt gatttaatga ctctgttgta
     5101 taggaagagt tgttcatcag ttttatttta gaaaattcgg ctagtggtaa atttcttagt
     5161 aaggccagca ttactttaat tatgtatcca tggcttacca atacaatcgt ttcattgttg
     5221 tgattttgcc gtatttcatc caggaaagca gccgatcgtt cagccacaga ttcaattgtt
     5281 tccatgtctt cgtaaatatc agtttcaaaa taaggctgtg gatagacctt tccttgtaat
     5341 acccccaggt ttctctccat taatctttca tcagtaataa tcctgctgct gttgttatgc
     5401 ttatgaatag cttgtgctgt ttgcagggca cggttgagag gacttgaata aattgtctga
     5461 aaagagaatt gtttcaactg tactcccaga ttttcgtttt cctttattcc cttttcagac
     5521 aaagtgccat gtttttgtcc ctggcaaata tttagtccat tctcaatggt ttccccatgc
     5581 cttgcaatta tcagcctggt catatttact cctttctgtt attctacatt aaccctgatt
     5641 ccttcagaat gtgaagaaaa ctcaggagca tacatacact gaatactggt tatcccattg
     5701 ctgaaattac ctgtatgggt ggcaaacaaa gtatattcaa atacataggt cccctttctc
     5761 aggtaagaga aaaagaagtt ggtactggcg tctttggtac tctcataata gccaaggccg
     5821 ccctgccatt tataactact cagtacgtta actggttcaa atgcggatgc ccgcatatct
     5881 ttcatatgca catattccat atccctgtcc actctcaatt ctacccgtac ttttattttg
     5941 tctccaacct ttatggcatc accgtcattc accggagtga taacaggacc acggtctgta
     6001 tttttttcta caaacaactt tttattgagt ttaagtggag tagaagcagt agtgatctta
     6061 tcaaggtctt caaaatattg ccagtacaca gcaccccatg taaccggaga ttgattattg
     6121 gcgctgctta cagaaactga gatattgccc atttctggtt ttaccaatct tccctctata
     6181 gttttcttga tatatccagt tccagcttct ttctcgtctg ttgttttgat aaaagccggt
     6241 ccaagcttga tttccacgat cggatcattt atcagccagt catttccttg taaaaggaga
     6301 gcataacagg cctctgcggt agctttagtg gttttccagt tattggtctg tttgttcttt
     6361 aataaccagg ttttcaggtc atcaattgtt ttattgtctt ttcctatttc atggaatgtt
     6421 tctatcagta aagaatgggt ttcgattggt gcttcatacc agaaccagcc atttctctgg
     6481 tctttccagt acatacccaa ctcttcgttg ctgatggatg tttctttcag cgaatttaat
     6541 atagcagaag gtgtctttgt gtctgccgtt ctgtatagtg ccagtgctgc cattccttgc
     6601 atatacttac tctgcccaac ccatgtttgc tgcatccggc tgcggaaata atcataggct
     6661 tttttagagg cggtagctat ttcataatcg gggaagaagc tgcgcatata taaatattgg
     6721 atagttgtat aaccgggtgt gtaggtttta aggtttgttt tgtacttgac caggttatcg
     6781 tagtcttctt ttatttgcct gtcgagatag ggtatggctt tggtcaatat agccttaaga
     6841 tatccttcct gtccgggagc aatnnnnnnn nnngctttca gttttttcaa atgaccgatt
     6901 ccagtgacga tatactgggt catatacctg tcgtctggcc cgcctttgaa ccatacaaaa
     6961 ccaccattgc tgctttgcat ttgtttcagc ttatcataag aactttgtaa ttgctcagcc
     7021 atttttatca tatcaaaaag cagggcgata tttttctttt gttcattttc attctttgcc
     7081 tgcaataccc atggtgtttc ttccagcagg atagctttta gttcctggtt tttttgaagg
     7141 ttactaagca atgcagctgt gtcttttgtt ttccattttt caaaaacttg tttgatcctt
     7201 ggtgatgaat tggcgataaa gcttgccagc gaatttgcat agtaacggtt ccatgtctgc
     7261 tctgcacaat cgtaggggta ttccataagg tagggaagag cctgtaccgc ataccacgca
     7321 ggattagaag tgtactccac tgtcaggctg tgatgctgga tgctttcact ttttccgctg
     7381 ttcagcagtt tttcaaagct gaaattttta gtaccggtcc ctctcatggg taagggcatc
     7441 gattctgtca ccagcatccg gttcgtcaat actggtagtg cattttcttc accgtcacta
     7501 tagctgccac ttttggcaat aattctccat accagtgctt tgttgaactg gtagggaata
     7561 tcaataggga attttaacgc ttcactttgg ccggcagcta ccgtaaaata ctggttggct
     7621 acactgttgt gaaaccagcc atctaccggt tcgccggtgg tggcatcaaa caactggagt
     7681 tcggctgtgc cggtcatctc cttatctgtg agatttacaa ctttggtgct gaattccatt
     7741 ttgtcacctt ctctcaggaa acggggagca ttgggttgca ccatcagttc tttttgcgta
     7801 attatttctt tggtgctgct gccaaaagct gcttctttgg tatgtgccag ggccatgaat
     7861 ttccacttcg tcaatgcttc gggcattgta aaggagaatt caatagctcc gctgctgtcg
     7921 gttcttaatt ccgggaggaa gaaggctgtt tcgttaaagt tcttccggat ttttatagaa
     7981 gcgtcttgtt gattattttt tttgtcatca attctatcag ttactccgtc accatcttta
     8041 tctctcatag ttactttata agcaacaatt tttgatggat cattcgtgtc agctactgct
     8101 gatgtatcgg ccactgctgg agccggagcc ccgcttgcct cctcacttac attcctcgtt
     8161 ctataataat aagtttgatt tccatattcc tcaatatcac tttgaaaaag gtaatcatag
     8221 gcttttgtta catactttga ttcttcagcg tacacatatt tttgatagga ctgaagctga
     8281 ctgaagttct gtgaaccgct ccatgtttta ttgttgtaat aataaggcca aatggagggc
     8341 tcattccagt tatgcgaata aaactggtct aatgatgcat catacatgct ggccagcatt
     8401 tctgcggcta ctttgtcttt cttgtatccg gtgattttta ttttccattt ttcttcgctg
     8461 ccgggcaatg ttttttcccg gtaggtcatg tattctattt gcagatcttt atttgtccag
     8521 ggaacattga tgctttgatt taactggtaa accctgttat gttttacaaa tagccagtta
     8581 gctccgaatc caccacggtc tgcttctgta gcagggaatg agaaggtttt tttctggttg
     8641 tttagttgca ggaaggagta gtcggtagtt gactgtttac tgttgaccat tgacttattt
     8701 acctggtgta caataaacag gttgtcagca gcagtaccta tttctactgt tgcttcctct
     8761 cccggttcaa cggttatttt ctttgctcct gtccacaaat aatcgggttg gttcagctgg
     8821 ttactttttt catctgtgag ttcgatgtat tttacatctt ttacttcctc tccgtttttg
     8881 tctttagtac ttatttcaac catgtagaac ccgggaagcc atgagccatg aacaattggc
     8941 catgagccat tcttgttaac tgaatctgct ttgctaaaaa cctgttcacc tttttcccaa
     9001 ctcttataat cactctcgtt atcatactca tcatgaggaa agtttttgat gtattcctct
     9061 ttactcatta caaactggtc aggtctttgc cagaaacgat tccggatcaa acgcttttct
     9121 tctttcagct tgctgatggt tacttttatg atagcaggtt catattcacc attcatgttt
     9181 tgtgtccgta tactcaacga ttttaagcta tctgcaggta acgtagacgg gacatttgcc
     9241 acaagcatca gtgacttata gcttacggaa acagattttt caccactgcg ggtctctcca
     9301 ttaatatctg ttacatctgc ataaactgta taatcaaaga ccggctcaaa ttttttatca
     9361 acttttaggt ctggtatagc cttgaattca aagctgaatt ttccgtcttt atccgttttc
     9421 gcttcaccat gggctatttc catttcatcg gaggggggct gccaccagcg ccaaaacagc
     9481 caggggtaaa taaacctcgg ttgccgtacc acccggtatt tcactgtggc gccgtcaata
     9541 ttgtttccgg cataagcttt tgcaataccg gttactttta tcatgtcatt tactttataa
     9601 gtgcctttta tagtttcgta atcaacataa aactttggcc gcttatattc ttccaccctg
     9661 aaactggcat tacctgcatc aggttttgta taaatattaa ataaaccgtt caggcctgat
     9721 tctggtaatt ggaacctgcc actaaaagag ccaaattcat ttgtctttat ttttattgta
     9781 tccaggtcct ggtaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta aaataaacag tctggcccgg
     9901 acggtaaata cttctgtctg taaaaaggtg gatacttgtg gtttcctttg catcttcagt
     9961 ttgcggatcc ctgtaatagt aataatcata cacaaagtca ttcataaaga gcctgtcgct
    10021 attatgcgtt acatccagca ggtaggcgta gttcccataa ttggtattgt cccgttttcc
    10081 tttctccatc cggaaaaagc cgttattgtc agtcaggtag gtttttcctt tttcttttat
    10141 gtacttacgc tggttgtaat catacttttg ctcccaaacc tgtacagctg ctttggcaag
    10201 cggctggcca ttatcccggt tcaatacaaa gaagtcatca ctgttgttta catagctgat
    10261 ggctgataca taaaacagcc ttgcgccaat cagggctttt tgggcgtcga ggtttttttc
    10321 attcgatgct accaggatat attcnnnnnn nnnnggtaat gcatcaattt ttatttctgc
    10381 actatgcgac tgcaggtcat tggtagaagg aagaacttgc tgccagttac ggataggtgt
    10441 cgctgctatt atagccggcc aatacttttc atcatattgg ttttccagaa gtttcttaag
    10501 gttatcgtcc gcttttataa gccttaggta aagcgtactg atatttttat aattaatcaa
    10561 tgacctgaaa ggctggccgg gtaagttaac tttttcaata ctgaattgca ggctgctgct
    10621 attgatctgt tgaagcaggt tgtggcagtt gatctttcct tccgaggaat ctttctgtag
    10681 cagtatcttt tcacagatct cttttgcttt tatccggcta aaacgccagg tggtatcacc
    10741 ataaggttta tactcattag ctttctcttg gtgccattca gcaagcaggt accaggcctg
    10801 cgaagcagca ggaaggtctc catattgttt tgcaatatga ttaattgcgt tgaagtacag
    10861 tttgtccttt tcaggatgtg ttgaattgct tttcacaaat tcaattcttt ccaggtcagc
    10921 gtcaatcaat gcatccggtt ttgcatcctt caggtgaaag gcgataagtt tttgatagat
    10981 caaaagagct ttgtgttgaa tggattgaga atcttttgta ggaaattttc ctgtaacaaa
    11041 atcagcagcg ggatcaaagg ctgaggcctt gtctatctca aaggcataag ccggtttttt
    11101 gatatctctt tcatcatttt caaagtaact taatgcccgg tgagcaagca gatcgtataa
    11161 ggtgggccgc aggtgtctta cgtttccttt tattatgata gcatcaaaag tctccagcct
    11221 tgattgctgc aatagcttct cttctttgat tgattgcagg tatagttccc cgattttttt
    11281 atggaaatct tctgctgtcc acgttgtgat atctttttta tcgaaatttt ctgttttggt
    11341 gcggttgtat aatttccacc ggttttgttg ataatagttc cagtacattg ctgcctgcag
    11401 gcttttgagg atggaggcag aaggttcttt gctttgtgtt atttctttct ctat
//
LOCUS       DAORLS010000071        11444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3022_length_11444_cov_1.808763, whole genome shotgun sequence.
ACCESSION   DAORLS010000071 DAORLS010000000
VERSION     DAORLS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_11444_cov_1.808763"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PK977_07190"
     CDS             <1..575
                     /locus_tag="PK977_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRF17934.1"
                     /translation="NGSLKRYYFNNDTLRFNPAGQAPNTDNTATVNLRPYFSEDGTYE
                     LIVTGKDRSNNEAGNIEYRVLFEVINKPMISNMLNYPNPFTTSTAFVFTITGNEVPQN
                     IKIEIMTVTGKIVREILKDELGPLHIGRNITEFKWDGTDQYGQKLANGVYLYRVVTNL
                     NGKSLDKYKAEGDNTDKYFNKGYGKMYLMR"
     gene            609..>2488
                     /gene="hscA"
                     /locus_tag="PK977_07195"
     CDS             609..>2488
                     /gene="hscA"
                     /locus_tag="PK977_07195"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRF17935.1"
                     /translation="MQPLFRFRKQDEAHKCYFCCFTKESDMAKIGINIATGSLQQQEM
                     IVGIDLGTTNSLVAIIHPESRKPVVLKEHDGNALVPSVIHFGEAGLVSVGDEAKKFLV
                     TEPHNTIFSVKRLMGKSYNDVRDYSSFFTYKVIDDNSESLVKVQVGDTFYSPLELSSY
                     ILKELKTRAEHILKTPVTKAVITVPAYFNDAQRQATRDAGRLSGLDVLRIVNEPTAAS
                     LAYGLGLRNDETKTIAVYDLGGGTFDISVLRITGGIFEVLSTNGDTYLGGDDFDRAIA
                     EYWSAKLGLTNNELNANKNIAQELRLLAEEAKKQLSFTPEFKGSIAGEELLLTREEFE
                     KLAKPFVERTLQCCKNAMQDAQLNIVDIDEVVMVGGSTRVPLVKNSVAEFFGRALHDS
                     LNPDEVVALGAAIQADILAGNNKDFLLLDITPLSLGIETMGGLMDVLIPRNSKIPSKA
                     GRQYTTQKDGQTGIRISVYQGERDLVKDNRKLAEFNLTGIPVMPAGLPKVEISFLVNA
                     DGILVVKAKELRSGVEQTIEVKPQYGLTDEEVEKMLLDSITHAKEDIQTRALVEAQTE
                     GQHILDTTRQFIEKNRVFLQQEEIEITEASMKDLEQKLFSTDKDAIQSAIERLNVISR
                     PYA"
     assembly_gap    2489..2612
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2613..3565)
                     /locus_tag="PK977_07200"
     CDS             complement(<2613..3565)
                     /locus_tag="PK977_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17936.1"
                     /translation="MIYCLLKQSWAGVLALSAILSLSHPFTGIELLGITSAWLLTEKI
                     IFKNKHIPDWLLPGELLILLFHLYYYLYHLNQFDDHRSVSEQYSLNWRLRYFSIIPAY
                     CITGLLALISAFKLNKPGIFFSQSQNRLFLLWFLIAFSLANHEIFMKPMQPLHFTRGY
                     IWTSLFVLGVPALHYFLIKLTASFIKRIIATSFLLLFLSDNLIWIFDQVKSKADVPST
                     SYINSEQKKIFDLLNKHTTNKTLIIGTDELVPYLSTIYTKAYPWFSHIFTTPFAEQKK
                     AALSRFIESGETDPSWEKRELIFVIDKKNDRFKKRVETLSNT"
     assembly_gap    3686..3836
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3837..4136)
                     /locus_tag="PK977_07205"
     CDS             complement(<3837..4136)
                     /locus_tag="PK977_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17937.1"
                     /translation="MIKTRNWLFALLLTTPMLVFFLGYLFNHTKELKPTGFIQYDNIS
                     YVAYAKQYLDSEKGSIFFSNPFNDNDNYQPIYFQTQTLLFAGFLKMGIIPGWILIP"
     gene            complement(4129..>4497)
                     /locus_tag="PK977_07210"
     CDS             complement(4129..>4497)
                     /locus_tag="PK977_07210"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRF17938.1"
                     /translation="LFERIGRVREINDHTTGAVSVLKFEPNKIELEITASNDGLLNLF
                     QQYHHGWRAFEDNIEIPVYCTNQAFISVPVSAGKHSIQFKFEPHNFIRVAVCTSLLTL
                     LLIAIFFILQFLKREHPTDD"
     assembly_gap    4498..4566
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4567..6254)
                     /locus_tag="PK977_07215"
     CDS             complement(<4567..6254)
                     /locus_tag="PK977_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17939.1"
                     /translation="MQKISTFLLSRKHYLLLLLITLVAYWPLTFHIFSLKNDALVYFL
                     PYRYHISEAIQNGHFPWWNPYLYTGLPLHSDIQSGVWNPVVMFISLFTGYNLTVLQWE
                     LLFYLFIAAAGMFKLTKEFNNSVTTALIAATAYLCCGFMTDSGSIIPWITSAAYLPFV
                     FLYFYRLLNNPKLNTALKLSLALSLLLTAGYPSFFIYCLYILLIGFIIWQVSHYKERS
                     VKSLFLFSGITMLIFFLIVSPAVFSWWDFFDYYERGSGASLDRIQTNAFPPFSSLSYL
                     FPSAVSKSHPLLQTDLTARNASTGIFIFLFFLLALAGKPKPVYKFISALILFSFLFSL
                     GDATPIREWCHRFLPFMNTFRHPASIRVFTSIGIILIAAPTLNGFLSMKEPVTKNINL
                     ITALILILLSGVLIFYLPQSNLPDFSENENPKALLDKLSFVDIAVLQGIIQILFISLF
                     LLAIWKGFKKYIPALAIINSIVFCWMALPFTFISQVKTSVIDQYIASFPKNYPLNEIA
                     KPVITTSTDSSTVSPLGQKKLYDKTISIQDHIITPTINSNYINFLKNNPLRTAL"
     gene            complement(6256..6966)
                     /locus_tag="PK977_07220"
     CDS             complement(6256..6966)
                     /locus_tag="PK977_07220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF17940.1"
                     /translation="MSTDYKYQGEELTLFETATNWKNYFASHISSYLTGKVLEVGAGI
                     GGTTLLLNKGKAERWLLLEPDETMQQLLQQKINDKLLPSNCIAVKGTLDSLGKNERFD
                     CIIYIDVLEHIENDADELKNATDLLAQGGYLVVLSPAFQFIFSPFDKAIGHFRRYSRK
                     TLSAIRPASLIREKMIYLDSVGFFASLLNKILLRQSYPVKKQVQLWDKWMIPSSKITD
                     RIFLYSFGKSILAVWKKK"
     gene            complement(6963..>7845)
                     /locus_tag="PK977_07225"
     CDS             complement(6963..>7845)
                     /locus_tag="PK977_07225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF17941.1"
                     /translation="TELAKAFSNLKDYRFRILVVNDGSTEELNITTNTFFPIQILHLQ
                     RNIGHQKALSIGLAYIKENISCDRVLIMDTDGEDRPEDALELLLAAENRNGDIIFAHR
                     GSRREDFLFKLFYWLYKISFRFLTGRKISFGNFMIMPKDSLNKLVYYSEIWSHLAGGI
                     LKSGLAYSTIQTHKGTRYAGKSKMNFSKLLLLGLGAISVFMEIIASRLLIFSCCLIGF
                     SLLIILTLLGVKTFTSLAIPGWTSTVMSSMLIVLLQSLLLSLVTMFLYFSSESQRKFV
                     PVYHYKDYTGAVETFTA"
     assembly_gap    7846..7862
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7991..9082)
                     /locus_tag="PK977_07230"
     CDS             complement(7991..9082)
                     /locus_tag="PK977_07230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRF17942.1"
                     /translation="MTAETPKRSHYPALDGLRGVAILLVVLYHNFGFIKQSYFGWLGV
                     DLFFVLSGFLITDILMNAVGKTNFLRNFYMRRMLRIFPLYYLSLIIFLFIIPVFTSPT
                     IKWDYYTNNQFFLWTYLQNWLYIFKDPKNAQILNHYWSLAVEEQFYIFWPLVILLLKK
                     PKYLLIFICTLLSLVILFRFGLWIYKIENLAYYNLYTFTRIDGICIGCLVALLKHMNF
                     EFIKKNTAIIVLSFAAINFLFYFINRFYSFSFPYTGLVGYTTFAMMFGLLVYEAVNRE
                     TKIINLIFNIPLLKFFGRISYSFYIFHWPVYLVMNSYLLDMVKKQIPGIGAQLTISIT
                     AAGCTILLSWLSYHYFESYFLKLKTKYSH"
     gene            complement(9098..9427)
                     /locus_tag="PK977_07235"
     CDS             complement(9098..9427)
                     /locus_tag="PK977_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17943.1"
                     /translation="MGDRLDGHIVQGHVDAVAICKNVIEKNGSWEYTFEFPKKFASLI
                     IEKGSIAINGISLTAFSVKGKNFTVAIIPYTFEHTNINKVTKGTIVNLEFDMIGKYLL
                     RKLSLKK"
     gene            complement(9447..9680)
                     /locus_tag="PK977_07240"
     CDS             complement(9447..9680)
                     /locus_tag="PK977_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17944.1"
                     /translation="MFTGIVESTGIVKEVISNGSNRTFWIESPISRQLKVDQSVSHSG
                     VCLTVEDLNCNSHRVTAIKETLKSGGLVCWHQC"
     gene            9779..9976
                     /gene="rpsU"
                     /locus_tag="PK977_07245"
     CDS             9779..9976
                     /gene="rpsU"
                     /locus_tag="PK977_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRF17945.1"
                     /translation="MLIIDSKDCENIDKALKKYKKKFEKSKVLLQLRERQSFTKPSVK
                     RRGEVLKAVYKQKIASGKIEG"
     gene            10044..>10403
                     /locus_tag="PK977_07250"
     CDS             10044..>10403
                     /locus_tag="PK977_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRF17946.1"
                     /translation="MPLISLQTIQPYIEYLKFEKRYSAHTLTAYKADLYEFTEFVKTQ
                     FGETDLASIHHGVVRSWLARLKEQGLAAKSLNRKISTLKSFFKYLIKNGVIDISPMAK
                     VISPRIGKRLPVFVKEEE"
     assembly_gap    10404..10429
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10430..10952
                     /locus_tag="PK977_07255"
     CDS             <10430..10952
                     /locus_tag="PK977_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF17947.1"
                     /translation="LNSSTENWRSLNARMIITILYATGLRLSELINLKEKQVDFARLQ
                     IKVLGKGNKERIIPLSDKLINGIREYQQLKRIEFETYEDVLLVSEKGKKLYPKYAYLI
                     VNQYLGKSSTLDKKSPHVLRHSFATHLMNNGADLNAVKELLGHSSLAATQVYTHNTIE
                     KLKDIHKKAHPKS"
     assembly_gap    11041..11090
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11091..11419
                     /locus_tag="PK977_07260"
     CDS             <11091..11419
                     /locus_tag="PK977_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRF17948.1"
                     /translation="LLFHYKMMTMNVQIQTVHFNADSKLIEYVNRKLEKLNTFSDRII
                     QVNVFLKLDNVVHTIKDKIAEIRVHVPRHNFFVKSTSKSFEESFDDAFETMVAQIKKS
                     KEKKAA"
BASE COUNT         3698 a         1935 c         2275 g         3099 t
ORIGIN      
        1 cgaatggcag cttaaaacgt tattatttta ataatgacac attaagattt aatcctgccg
       61 gacaagcacc gaatacagac aatacagcca cagttaatct aaggccgtat ttctcggagg
      121 atgggaccta tgaactcatc gttactggta aggatagaag taataatgag gctggtaata
      181 tcgaatacag ggtgcttttt gaggttatta ataagccaat gatttcaaac atgcttaatt
      241 atcctaatcc atttacaacc tcaactgcat ttgtttttac cataacgggg aatgaagttc
      301 ctcagaatat taaaattgag atcatgactg tgaccggaaa gattgtcaga gagatattga
      361 aagacgaatt aggccccctc catatcggac gaaatattac tgaattcaag tgggatggca
      421 cagaccaata tggccaaaag cttgctaatg gtgtatactt gtacagggtg gtgaccaatt
      481 tgaatggcaa atcactggac aagtataagg cagaaggtga taacacagat aagtatttta
      541 ataaaggtta tggtaaaatg tacctgatgc gataaattaa atgtagttaa aagaataggg
      601 gttgcattat gcagcccctt ttccgttttc gtaaacaaga cgaagcgcat aaatgttact
      661 tttgctgttt cactaaggaa agcgatatgg caaaaattgg tattaatata gcgaccggaa
      721 gtttacagca gcaggaaatg atcgtaggta ttgatctggg gacaacgaac agcctggtag
      781 ccattataca cccggaaagc aggaaaccag tggtcctgaa agaacatgat ggtaatgcgc
      841 tggttccttc cgttatccat ttcggtgaag ccggtttggt atctgttggg gacgaggcta
      901 aaaaattcct tgttactgag cctcacaata cgatcttttc tgttaaaaga ctgatgggta
      961 aatcctataa tgatgtaagg gattattctt cttttttcac ctacaaagta attgatgata
     1021 atagtgagag cctggtaaaa gtgcaagtag gggatacgtt ttattcacca ttagaattat
     1081 cttcttatat acttaaagaa ttaaaaacaa gggcagagca tatactgaaa acaccggtaa
     1141 caaaagctgt gatcacggtg cctgcatatt ttaatgatgc tcaacgtcaa gcgaccagag
     1201 atgccgggcg tctttctgga ctcgacgttt tacggatagt gaatgagcca acggctgcta
     1261 gtcttgctta tggtttgggt ttacgaaatg atgagacaaa gacaattgct gtatatgatt
     1321 tgggtggcgg caccttcgat atctccgtgt tgagaattac cggaggcatt tttgaagtat
     1381 tgtcaaccaa cggagatact tacttgggtg gtgatgattt cgacagagct atagcagaat
     1441 actggtctgc aaaattgggc ctgacgaata atgaattaaa cgccaataag aatatagcac
     1501 aagagctacg tttgctggct gaagaagcaa aaaaacaact ttctttcact cctgaattta
     1561 aaggcagcat agccggggaa gaattgttgc tgaccaggga agaatttgaa aagctggcaa
     1621 agccattcgt tgagagaaca ttacagtgct gtaaaaatgc aatgcaagat gcacagctaa
     1681 atattgttga tatagatgaa gtggtcatgg ttggggggtc tacaagagtg ccacttgtaa
     1741 aaaactcagt ggccgaattt ttcggcaggg cattacatga ttctttgaat cctgatgaag
     1801 tagtggcact cggagctgca atacaggcag atatactggc tggtaacaat aaagattttt
     1861 tattgctgga tattacgcca ctctcattag ggattgaaac gatgggtgga ttaatggatg
     1921 tattgatacc ccgtaattct aagatacctt caaaggctgg caggcaatat accacacaaa
     1981 aagatggtca gaccggcatt cgaatatcgg tttaccaggg cgagagagac ctggtaaaag
     2041 ataaccggaa attggccgaa tttaatctca ccggtattcc cgtgatgccg gcaggcttgc
     2101 cgaaagtaga aatatctttc ctggtaaatg ccgatggtat cttagtggtt aaagcaaaag
     2161 aattgcgcag tggggtagaa caaactattg aggtaaaacc gcagtatggt ttaacggatg
     2221 aagaagtgga gaaaatgcta ctggattcca ttactcatgc aaaggaagat atacaaacaa
     2281 gggcattggt agaagcgcaa acagaggggc aacatatact ggatacaacc aggcaattta
     2341 tagaaaagaa tagagttttt ctgcaacagg aagaaatcga aattacagaa gcctcaatga
     2401 aggatctgga acaaaaatta ttttcaacgg ataaggatgc tatacagtca gcaatagaaa
     2461 ggctgaacgt aatttcaagg ccttatgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgtttga aagtgtttca actctttttt
     2641 tgaatcgatc gtttttttta tcaataacaa agattaattc tcttttttcc caagatggat
     2701 cagtttcacc cgactcaata aatcgggata gtgctgcttt tttttgctca gcaaatggag
     2761 tggtaaatat atgagaaaac catgggtagg ctttagtata aatagtactc aagtaaggta
     2821 ctagttcgtc tgtgccgata atcagtgtct tgtttgttgt gtgtttattt aacaggtcaa
     2881 atattttttt ttgctcactg tttatatatg aggttgatgg aacatcagcc tttgatttga
     2941 cctggtcaaa aatccatatg aggttgtcag aaaggaaaag tagtaaaaaa gaggtagcta
     3001 tgatcctttt aatgaaggaa gctgtcagtt tgattagaaa ataatgtaat gccggaacac
     3061 ctaatacaaa caaagacgtc cagatataac ctcgtgtaaa atgaagcggc tgcattggtt
     3121 tcataaatat ttcatgatta gcaagactaa atgcaattaa gaaccaaagc agaaagaggc
     3181 ggttctgact ttgagaaaaa aaaatgccgg gtttgttcaa tttgaatgcg cttattagcg
     3241 ccagcaaacc agtgatgcag taagcaggga tgatactaaa ataccggagt cgccagttaa
     3301 gagaatattg ttcacttacg gaacggtgat catcaaattg attaagatga tacagataat
     3361 aatacaagtg aaagagcaat attaatagtt caccaggtaa aagccagtca ggaatatgtt
     3421 tgtttttgaa aattattttt tcagtcagca gccaagcact tgtaataccc aggagctcaa
     3481 ttccggtaaa aggatgcgat aaagaaagaa tggctgacag ggcaagcacc ccagcccaac
     3541 tttgttttaa tagacaataa atcactgaaa gaaatagcat atgataatag gcctcacacg
     3601 aaaaaaaaag tgatctgcca aaattgagcc cccaccagcc tgaaccgggg tcaagaatga
     3661 atatgctgtt aactattgaa gagccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggt
     3841 ataagaatcc acccgggtat gattcccatt tttaagaacc ccgcaaaaag caaagtttgc
     3901 gtttgaaagt atataggctg ataattgtca ttgtcattga acgggttact gaaaaaaata
     3961 ctaccctttt cgctatcaag atattgcttg gcataagcca cataagatat attgtcatat
     4021 tgtataaaac cggttggctt cagttctttg gtatggttaa aaagataacc aagaaagaaa
     4081 accagcattg gagtggtcaa taacaaggca aataaccagt ttcttgtttt aatcatcagt
     4141 cggatgttct cttttaagaa actgtaagat aaagaatata gcgatcagca acagggttaa
     4201 taaggaagta catacggcaa cccggataaa attatggggt tcaaatttga attgtataga
     4261 atgtttccct gctgataccg gtacgctgat aaaagcctgg ttagtacaat aaacaggtat
     4321 ttcaatatta tcttcaaaag cccgccagcc atggtgatat tgctggaaca ggtttaacaa
     4381 cccatcatta ctggcagtaa tttctaattc aattttgttg ggctcaaatt ttaaaacgga
     4441 aacggcacca gtcgtatgat cattaatttc ccttaccctg ccaatacgtt caaataannn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnttca gtgctgttct caacgggttg tttttaagaa aatttatata gttgctgttg
     4621 atagtaggtg ttattatatg atcctgtata ctgattgttt tgtcatataa ttttttctgc
     4681 cccaggggag atactgttga tgaatcagta ctggtagtga ttacaggctt tgctatttca
     4741 ttgagcggat agttctttgg aaatgaagct atgtattggt cgataactga tgtcttaacc
     4801 tgtgagataa aagtaaaagg caaagccatc cagcaaaaaa caatgctgtt aataattgcc
     4861 agggcaggga tatatttttt aaaccctttc caaatggcca aaaggaatag actgataaat
     4921 aggatttgaa tgatcccttg cagtaccgcg atatcgacaa aactcagctt atcaagaaga
     4981 gctttggggt tttcattttc tgaaaaatct ggcaaattac tttgtggcaa gtaaaagatc
     5041 agaacgcctg aaagcaaaat aagaattaaa gctgttatta gatttatatt ttttgtaaca
     5101 ggttctttca ttgagagaaa cccgttcaat gtaggagcag caattaaaat gataccaatg
     5161 ctggtgaata cacgtatgga agctgggtgc ctgaatgtat tcataaaagg gaggaaacgg
     5221 tggcaccatt ctcttatcgg ggtggcatca cccaggctga aaagaaaaga gaagaggatt
     5281 agggcactaa taaatttata aacaggtttc ggttttcccg caagtgccag caggaagaac
     5341 aaaaaaatga atattcctgt tgatgcattt ctggcagtca gatcggtttg caataaggga
     5401 tgacttttag atacagcaga aggaaacaaa tacgataaac tgctgaaagg aggaaatgca
     5461 ttggtttgta tcctatccag gctggccccg ctcccccttt cataatagtc aaaaaaatcc
     5521 caccaggaaa aaacagcagg actgacaata agaaagaaaa taagcatggt aataccggag
     5581 aataaaaaaa gagatttaac tgatctctct ttatagtgac tgacctgcca gataataaat
     5641 ccaataagaa gtatatagag acagtagata aaaaaactag gataacctgc tgtgagtaat
     5701 aagctaagtg caagagataa ctttagggca gtgtttagtt tgggattatt taacaggcgg
     5761 tagaaataga gaaaaacaaa aggaagataa gctgcgctgg tgatccaagg aatgatggaa
     5821 ccgctgtcgg tcataaaacc acagcatagg tatgccgtgg ctgcaattaa ggcagttgtt
     5881 acgctattat taaactcttt tgtcagctta aacatcccgg cggcagcaat aaacaaatag
     5941 aatagtagtt cccattgaag aaccgtgaga ttataccctg tgaaaagaga aatgaacata
     6001 acgacaggat tccatacccc gctttgtata tcgctatgta gagggaggcc tgtatacaag
     6061 taggggttcc accaggggaa atggccgttt tgtatcgctt cgctgatatg gtagcgataa
     6121 ggaagaaaat aaactaaagc atcattcttg aggctaaaaa tgtgaaaggt caggggccaa
     6181 taagcaacta gggtaatcag tagtaaaagc aggtaatgct ttcggcttaa aagaaaagta
     6241 ctgatctttt gcatattatt tcttcttcca aacagccaaa atactttttc caaaggaata
     6301 taagaaaata cgatccgtga tttttgaact tggaatcatc catttgtccc atagctgaac
     6361 ctgtttcttt acagggtatg attgccgtaa taatatttta ttcaataaag atgcaaaaaa
     6421 acctaccgag tcaagataaa tcatcttttc ccgtattaaa gaagcgggcc gaattgctga
     6481 caatgttttc cggctatatc tcctgaagtg tccaattgct ttgtcaaaag ggctaaatat
     6541 aaactggaaa gcaggagaga ggacgacaag ataccctccc tgagccagga gatcagttgc
     6601 attctttagt tcatctgcat cgttttctat atgctcgagc acatctatat aaatgataca
     6661 atcgaacctc tcgttcttgc ccaatgaatc aagtgttcct tttactgcta tgcaatttga
     6721 ggggaggagc ttgtcattta ttttctgttg aagtaattgt tgcatggtct catccggctc
     6781 caatagcaac cacctttcag ctttaccttt gtttaataaa agggttgttc caccgatacc
     6841 ggccccaact tccagcacct ttccggtcag gtatgaggat atgtggctgg caaaatagtt
     6901 tttccagtta gtggctgttt caaaaagcgt taattcttct ccctggtatt tgtaatctgt
     6961 actcatgcag tgaatgtttc aacggcaccc gtataatctt tataatgata gaccggtaca
     7021 aatttccgct gggactcgga tgaaaaatac aggaacatcg taacaagtga tagcagtagg
     7081 ctctgaagca gcacaataag catagatgac ataacggtgg atgtccagcc cggaatagct
     7141 aatgatgtga aggttttaac accaagcagg gtaagaataa tcagtaaaga aaaaccaata
     7201 agacagcagg aaaaaatcag caggcgactg gcaattattt ccataaatac agaaatggct
     7261 cccagtccta gtagtaacaa tttactgaag ttcattttcg atttcccggc ataccttgtt
     7321 cctttatggg tttgtatagt tgaataagca agccctgatt ttaaaattcc accggcaaga
     7381 tgactccata tttcactgta ataaacaagt ttattaagag aatctttagg catgatcata
     7441 aaattcccaa aagatatctt acgaccagtc agaaacctga aactgatttt ataaagccag
     7501 taaaataatt tgaatagaaa gtcttcccgg cgggaacctc gatgagcaaa gattatatca
     7561 ccgtttctat tttctgcagc cagcaataat tcaagcgcat cttcaggccg gtcttctcca
     7621 tctgtatcca tgatcaatac cctatcgcag ctaatattct ctttaatata tgctaaaccg
     7681 attgacagag ctttctggtg cccgatattc cgttgcaaat gaagtatctg tataggaaaa
     7741 aatgtatttg tagtgatatt caactcttct gtagagccat catttacgac taaaattctg
     7801 aaacgataat ctttcagatt tgaaaatgct tttgccagtt cggttnnnnn nnnnnnnnnn
     7861 nnagttcggt taagagccgg ctcagtgatt ctccatcgtt aaataagggg cacaatatgg
     7921 tgacattctt ttgcatggac tggtttaaag atacaatgca gggtttttat aatgaaatga
     7981 ctgcggaaga tcagtggctg tacttcgttt taagttttaa aaaataactt tcaaaataat
     8041 gataactgag ccagctcagc agaattgtac agccggctgc ggttatggaa atagtcagtt
     8101 gagctcctat gccgggtatt tgttttttaa ccatatccag taaataacta ttcataacga
     8161 ggtataccgg ccagtgaaat atgtaaaatg aataggagat acgtccaaaa aatttgagta
     8221 aagggatatt aaaaattaag ttgatgatct tagtctccct gttaaccgct tcatacacta
     8281 ataagccaaa catcatggca aaagtagtat agcctacaag gcctgtatat ggaaatgaga
     8341 atgagtagaa acgatttatg aaatagaaaa ggaaattaat tgctgcaaag ctcagaacta
     8401 tgattgctgt attctttttg atgaactcaa aattcatgtg ttttaataaa gcaacaagac
     8461 agccaataca aataccgtca atacgggtga atgtgtacag gttgtagtaa gcgaggttct
     8521 caattttata gatccacagg ccaaagcgaa acaatatgac cagtgataaa agcgtacata
     8581 taaaaatcaa cagatacttc ggttttttca gtaaaagaat cacaagcggc caaaaaatat
     8641 agaattgttc ttccactgct aatgaccaat agtgattcag tatttgagca ttcttggggt
     8701 ctttaaatat atataaccag ttctgcagat aggtccacag gaaaaactgg ttattagtat
     8761 aataatccca tttaatagtt ggggaagtaa agaccgggat tataaatagg aatataatta
     8821 ggctcaagta gtacaatggg aaaatccgta acatccgccg catataaaaa ttgcgcagga
     8881 aattagtttt ccccacagca ttcatcaata tatctgtgat caggaaaccc gaaagcacaa
     8941 aaaacagatc tacccctaac cagccgaagt aggattgttt tataaatcca aaattgtggt
     9001 acagaactac taacaaaatg gcaactcctc taaggccatc cagggcaggg tagtgagacc
     9061 ttttcggggt ttctgcagtc atttattggg ttagttatta cttcttcaaa gataactttc
     9121 tgagcagata cttaccaatc atatcaaact ctaggtttac tatggtaccc ttggttactt
     9181 tattaatatt ggtatgctca aaggtgtagg ggataatagc tacggtaaaa ttttttcctt
     9241 taacactaaa agcagttagg ctaatgccgt ttattgcgat tgaacctttc tcaataatga
     9301 gtgaagcaaa ttttttggga aattcaaatg tatactccca actaccattc ttttcaatga
     9361 catttttgca tattgccact gcatctacgt gtccctgaac aatatgcccg tctaaacggt
     9421 cacccaactg aagggacctc tccaggttaa cattggtgcc agcagaccag tcccccagat
     9481 ttgagggttt cctttatggc agtaacccgg tgactattac aatttaggtc ctcaactgta
     9541 aggcatacac cactatggct cacactttgg tctaccttta attgtcgtga aattggcgat
     9601 tctatccaaa atgttctgtt tgaaccattg gaaatgacct ctttaacgat acctgttgat
     9661 tcaacaattc ctgtaaacat aatagcaaag taattcaatt cttttttgat ttcaggtttt
     9721 gaaatgaacc cattatcttt gccaacctaa aaaataccaa aggaggtatc atttaattat
     9781 gcttatcatc gactcaaaag attgcgaaaa cattgacaaa gcgcttaaga agtacaaaaa
     9841 gaagtttgaa aagtcaaaag ttttattaca attgcgtgaa cgtcagagct ttacaaagcc
     9901 ttccgtaaaa cgtcgtggtg aagtgctaaa ggctgtttat aagcagaaaa tagctagcgg
     9961 aaagatcgaa ggttaattta atcttcagca aaatattcaa ggatggctgc aaagttttaa
    10021 tagctttgta gctgtctttt tttatgccct taatttccct gcaaaccatt cagccctata
    10081 ttgaatacct gaaatttgaa aaaaggtact ctgctcacac cttgacagca tacaaagctg
    10141 atttatatga atttacagag ttcgtcaaaa cccaattcgg ggaaactgac ctcgcatcca
    10201 ttcatcatgg agtagttaga agctggcttg cccggctaaa agagcaaggg cttgcagcaa
    10261 aatctctgaa taggaaaatc tccactttaa aatctttttt taaatatctt attaaaaatg
    10321 gggttatcga tataagccca atggccaaag tgattagccc cagaattgga aaaaggttac
    10381 cggtttttgt aaaagaagag gaannnnnnn nnnnnnnnnn nnnnnnnnnt cttaatagtt
    10441 caacagaaaa ttggcggagt cttaatgcca ggatgatcat tacaatatta tatgcaactg
    10501 gtttgaggtt gagcgaattg ataaatctca aagaaaagca ggtggatttt gcccgtcttc
    10561 aaattaaagt gcttggaaaa gggaataaag agaggataat ccctcttagc gataagttga
    10621 tcaacggtat cagggagtat cagcagttaa aaagaataga gtttgaaact tatgaagatg
    10681 tcctgctggt gtctgaaaaa ggcaaaaaac tataccccaa atatgcttac ctgatcgtaa
    10741 atcaatattt gggaaaatca agcacactgg ataaaaaaag cccccatgtt ttgcggcaca
    10801 gctttgccac acatttaatg aataatggtg ccgatctaaa tgcagtaaaa gagctgcttg
    10861 gacatagtag cctggcggca acccaggtat atactcacaa tacaattgaa aagctgaaag
    10921 atatacacaa aaaggcccat cctaaatctt aaaaggcctg tactcacaat aaaaaattta
    10981 gaaaattttg tttgggagag gttctctttt gcagtaactt gtaggtgtaa aaagaattaa
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atttattgtt
    11101 tcactataaa atgatgacca tgaacgtaca aattcagaca gttcatttca atgccgacag
    11161 taaactgatt gagtatgtga accgaaaact ggaaaagctg aataccttca gtgatcgtat
    11221 cattcaggta aatgtcttcc tgaaactcga caacgtagtt cataccatta aagacaagat
    11281 tgcagaaatc agggtacatg ttccccggca caatttcttc gtgaaatcaa ccagtaaatc
    11341 ttttgaagag tcgtttgatg atgctttcga aacaatggta gctcaaataa aaaaaagtaa
    11401 agaaaaaaaa gccgcttaaa acattgtaag ccccgccccg gcgg
//
LOCUS       DAORLS010000072        11427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3026_length_11427_cov_1.978104, whole genome shotgun sequence.
ACCESSION   DAORLS010000072 DAORLS010000000
VERSION     DAORLS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_11427_cov_1.978104"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>791
                     /gene="sprA"
                     /locus_tag="PK977_07265"
     CDS             <1..>791
                     /gene="sprA"
                     /locus_tag="PK977_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF17949.1"
                     /translation="GLFPESSLINDLRSGYNRAKIAWYNIEPVLQEKRNPNNPLRNDL
                     AELSRPETRQVLQREIFPRRSTQFGEGLLTTFDIAFYPKDKGPYNLEYRNTRINANGK
                     LTNPKQSWGGMMRNIDQTDFETSNIEFIEFWLQDPFILNNSNPTGGQLYFNLGNISED
                     ILRDGKRQYENGLRTPTNNAALDSNTVWGQVPANPMQITNGFSILPEDRQYQDVGYDG
                     LQDDAERRRFSQYLTDLAATFGTGSLIYQQALSDPAADNFRAYRD"
     assembly_gap    792..857
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <858..>1067
                     /locus_tag="PK977_07270"
     CDS             <858..>1067
                     /locus_tag="PK977_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17950.1"
                     /translation="NPHGNSPIAENNTQFVNAFTQYPDAEELNRDNTMNEVEEYFQYK
                     VDIQPNMPVGSNFITDKRVVTVNLAD"
     assembly_gap    1068..1279
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1280..>2122
                     /gene="sprA"
                     /locus_tag="PK977_07275"
     CDS             <1280..>2122
                     /gene="sprA"
                     /locus_tag="PK977_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF17951.1"
                     /translation="TGNYTQLPLNDPTNVNTLAVNVEENDQRQPIPYTIPPGIERQQQ
                     LSNNNVALFLNEQALSVQVCGLPQNEARGVFKTMNLDMRQYGRLSMFIHAESVQNAQP
                     IEDGQMRAVVRIGNDFSGNYYEVKIPLKITPFGTTDSLTIWPAVNNLDFQLELLTQLK
                     SRRNKQAVPPSQYYSETSADGRTYAILGNPNLGEVRGMLLGVENLAQESMCTEVWFNE
                     LRLSQLDEEGGWAALARADIKLADLGNISLAATARTRGFGTLEQRVNERSREDVYTVD
                     ATAT"
     assembly_gap    2123..2231
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2232..>3973
                     /gene="sprA"
                     /locus_tag="PK977_07280"
     CDS             <2232..>3973
                     /gene="sprA"
                     /locus_tag="PK977_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF17952.1"
                     /translation="DPYDLDIKLRDKLKEVDGKERDSIKRESLDVTTTKTFNLTNVKK
                     IKTDGKRPKIWSVTNFDFNYSYIQTLSYNPLIERDEMRRTRGAVGYTYAPQVQPFEPF
                     KKLIKSKSKWLALLKEFNLNYAPSQISFRADVFRQFGATRPRNVGGGPYKIPETYNKY
                     FTFDRFYILQWNLTKSISIDFNAVNNARIDEPFGRIDNKVAKDSVRKNLLKGGRNTSY
                     RQEVTITYNVPMQKIPLLDWTTLRASYNTKYNWLAGSLLARTPEIDLGNTLSNTQTRT
                     INGEMNFEMLYNKSRFLRAVNTSAPKQPPANNQPGGDKNQPGGKGDNKKEDKKSKNNK
                     TKQGDNAGAQQKDQQQQQVVPGMPSQQDTSKAKLSKKERRKLAKLERKQQRLQKKQQR
                     KKDPNRPLPEISGVPKAFLQLATSLKRVGIQYTEDMGTTLPGYMDSTRVLGYNFKSRQ
                     PGFGYIFGYQPDTSWINRFGAKGLLSADTLVTAMIQQRYNQRLNLTAQVSPFRDFNID
                     INYDKTFDKQYSELYKDTSKFDNVGLTRLNPYALGSFNVSYIAFQTLFKKFDPNVVSE
                     TFQKFESNRAILSK"
     assembly_gap    3974..3986
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3987..>4347
                     /locus_tag="PK977_07285"
     CDS             <3987..>4347
                     /locus_tag="PK977_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17953.1"
                     /translation="NPYQGGVIDPNDPNFYKGYGRYAQDVVIPAFIAAYTNKDPNSVS
                     LIKNSNPNLRSNPFSGIKAKPNWTVTYTGLSRIPGLDKIFTNVSIRHGYHSTFSMNSF
                     NTALLFLDPFRVGYPSFT"
     assembly_gap    4348..4369
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4370..4892
                     /locus_tag="PK977_07290"
     CDS             <4370..4892
                     /locus_tag="PK977_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17954.1"
                     /translation="LTGNFIPYFLVPNITIQEAFDPLIEIDMTFTNQLQTRFEFRKSR
                     TLSLSLIDYQLAEQRSTEVTFGFNWRRKGLPLIKKLPFMKGEMDNDVTIRVDFSLRDD
                     ATANSKLDQRTAFGTAGQKVIRIAPSIDYVVNNRVSMKLFFEQNRNIPKISNAFPITN
                     TRAGLQVRVSLTQ"
     gene            complement(5120..5635)
                     /locus_tag="PK977_07295"
     CDS             complement(5120..5635)
                     /locus_tag="PK977_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17955.1"
                     /translation="MKKPYLLILMMTIMTACGYSQDVIRLQSCSNDMISKQADSLKNL
                     YGKDGYVLLKEASINMESEFEMPVIVPLTQGSWYQFVFIGDYSSRLYEVRMYDWQERM
                     VIFRQKKWGDIDGNVIVYSYVPQFSEFHMMKPVQVNKQKKKNLCGYVMLFKRSNDAAT
                     GNVSPSPNKPE"
     gene            complement(5850..6650)
                     /locus_tag="PK977_07300"
     CDS             complement(5850..6650)
                     /locus_tag="PK977_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF17956.1"
                     /translation="MNNELLTINQNSPTKIVFITGATSGFGKACAYKFAANGYDLIIN
                     GRRTDRLQQLADEIENKYNVAVLQLPFDVQNQTDTISSIENIPLEWKAIDVLINNAGL
                     ALGRDYFEEASFDDWNTMIDTNIKGFLYVAKAVAQLMAARKQGHIINMGSVAGKEVYE
                     KGNVYCATKYAVDALNQSMRIDLLRHNIKVTAINPGAAETEFSVVRFKGDEGTAKKIY
                     DGLQPLTAEDVAEVIYYAATLPAHVCINDLTISCLQQANAIYFNRQSS"
     gene            complement(6737..>7094)
                     /locus_tag="PK977_07305"
     CDS             complement(6737..>7094)
                     /locus_tag="PK977_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF17957.1"
                     /translation="VRYKLTVWDDANCPDSAFVTVTVFKTNPYVFVPTAFTPNNDGRN
                     DVIRPIAVGIQKINYFSIYNRWGERVFYTTANRAGWDGRVNGTPQASGVYVWMVSAVD
                     YLGKPVFLKGTVTLIR"
     assembly_gap    7095..7139
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7140..>7531)
                     /locus_tag="PK977_07310"
     CDS             complement(<7140..>7531)
                     /locus_tag="PK977_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17958.1"
                     /translation="FVVINTDPYPVANAGSPQVICYNTSTQLSGAHNGTRFTWTPANY
                     LNNPNILNPIASPPRTTSFVLTSWDDTPGCPKPGYDTVVITVQPKVRAYAGRDTTVIV
                     GQPLQLSGSGGVRYLWTPNTGLKNPNIA"
     assembly_gap    7532..7597
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7598..>8429)
                     /locus_tag="PK977_07315"
     CDS             complement(<7598..>8429)
                     /locus_tag="PK977_07315"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF17959.1"
                     /translation="DGFNVQFSHTSTGANSVFWDFGDLSTLADTSNINNPSYTYTDTG
                     TYTLKFVINRGTSCSDSAYRTVKVYPGFFPGFIYNGSCFTNPYQFTDTTNTRYGVVDS
                     WRWDFGDGTTLADTSRIRNPQYTYPSPGTRDVRLIVTNSKGCIDTAFVTINVLDKPLI
                     TLAFNDTLICVPDAVTLSASGTGTFNWTPLTNITNPNTPNPTVNPTTDTWYVANLTDN
                     GCVNKDSVRVRVISGVSLTAMPDTTICLTDAVTLNAVTNGLNFTWTPGATLNNPNILN
                     P"
     assembly_gap    8430..8456
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8457..8834)
                     /locus_tag="PK977_07320"
     CDS             complement(<8457..8834)
                     /locus_tag="PK977_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17960.1"
                     /translation="MPVNVICRNAPFTLNFSATDPDGDSLSYSLCSAYNGGAAINAGF
                     QDPAPPPYNSVPYNSPFTGINPLGTGATINPQTGVITGLAPDFGKYVVSVCISVFRNG
                     VQIATHRKDLIVQVSDCELTTATP"
     gene            complement(8970..9368)
                     /locus_tag="PK977_07325"
     CDS             complement(8970..9368)
                     /locus_tag="PK977_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17961.1"
                     /translation="MRRLVIAIFVFLFLSNTSFAAHIIGGEMRYVYLGPGSAPNTKSY
                     RIIMVLFRGDDPSGAALAPNYVVGIFNNDNGQKVIGTAANNNWLISQDPPGTPSVPII
                     FPSCIQNAPTLNYTYAIYTMVVELPNNSNG"
     gene            complement(9394..10185)
                     /locus_tag="PK977_07330"
     CDS             complement(9394..10185)
                     /locus_tag="PK977_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF17962.1"
                     /translation="MNKTISIKGKEIFYRVIGNGKPALLVHGFGEDGEIWHNQVKFLQ
                     DKFKLIVPDLPGSGRSELTDEMSMEGMAEVILAIMQEEKCVPCPVIGHSMGGYITLAL
                     AEKYPEVLTTFGLFHSSAFADSDEKKATRQKGISFIQQHGAGEFLKTVIPNLFAPESK
                     DKLSDTIKDLMEQGNNFNPHSLVSYYQAMMQRPDRTNVLRKAVVPVLFIMGKYDNAVP
                     MEDSLKQCHLPEKSYIHILSQSGHMGMLEEPGKCNRILEEFLLET"
     gene            complement(10188..>11427)
                     /gene="lnt"
                     /locus_tag="PK977_07335"
     CDS             complement(10188..>11427)
                     /gene="lnt"
                     /locus_tag="PK977_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRF17963.1"
                     /translation="CFEYIHLQDWGLSWPWLTLGNAFATKTEWVQWYEYTGTSGGTLW
                     VLGVNILLFLHLKKNMLVNRTRDYRFLIPAALLLVLPILVSKITTFRIEKGNQTNQNI
                     VVVQPNIDPYVKVSSEAGSFEAQLQMLISTTEKAADDNTALIVWPETALYMMTRMDEA
                     NLKGNYFLNPLWGFLKKHPKASLFTGIESYRQFSEKTKYSEEYNGFHYESYNGSVLLD
                     SNGANGFYHKSMLVPGVETLPWFLKFLSKWFDKFGGTTAGYAKQDHRTVIEEKNGYRI
                     APAICYESIYGEFMSRYIRNGANLICIITNDGWWKKTPGHKQHMSYARLRAIETRTWV
                     ARSANTGISCFIDPNGKVINPQPYNTNAAIKYNISAAKNKSNTFFVKFGDLLSKLMIG
                     ISILLTMTTIFLKIRKRTIN"
BASE COUNT         3185 a         2234 c         2377 g         3071 t
ORIGIN      
        1 atggattatt ccctgaatca tccctgataa atgatctgcg tagtggttat aacagggcaa
       61 agattgcctg gtataatatt gaaccggtat tgcaggaaaa aagaaatccc aataatcctt
      121 tgcgcaatga cctggctgaa ttatcaagac cagaaacaag acaggtttta caaagagaga
      181 tattcccccg ccgcagcacc cagtttgggg aaggactgct gacaacattt gatattgcat
      241 tctatccaaa agacaaagga ccctacaatt tagaataccg caatacgagg attaatgcta
      301 atggtaaact caccaatcct aaacaatcat ggggcggcat gatgcggaat attgaccaaa
      361 cggattttga aaccagtaat attgagttca ttgaattctg gttacaggat ccttttattc
      421 tgaataattc aaacccaact ggtggccagc tttactttaa cctgggaaat atttcagaag
      481 acattttaag agatggaaaa aggcaatatg aaaatggatt gcggacacct accaataatg
      541 cagccttaga ttccaatacg gtttggggac aagttccggc aaatcctatg cagatcacca
      601 atggattcag tattctgcct gaagatcgtc aataccagga cgtgggttat gacgggttac
      661 aggatgatgc ggaaagaaga aggtttagcc aataccttac tgaccttgca gctacttttg
      721 gaacagggtc tcttatatat caacaagctc ttagtgatcc tgcggctgat aacttcaggg
      781 cataccgtga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnaat ccgcatggca attcacctat agctgaaaac aacactcaat
      901 tcgtaaatgc atttacacag taccccgacg cagaagagtt gaaccgtgat aacacgatga
      961 atgaagtgga agaatacttc cagtacaaag tggacattca gccgaatatg cctgtgggga
     1021 gcaacttcat cacagacaag cgggtagtaa cagtaaacct tgctgatnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnt accggtaact acacccagtt gcctttaaat gatccaacta
     1321 atgtaaacac cctggcggtg aatgtggaag aaaacgatca gcgtcagcct atcccttata
     1381 ccattcctcc gggcatagaa agacagcagc aattgagcaa caataacgtt gctttgttcc
     1441 taaatgaaca ggctttgagt gtacaggttt gcggcctgcc gcagaatgaa gcaagaggtg
     1501 tgtttaagac aatgaacctc gatatgcggc agtacggcag attgtctatg tttattcatg
     1561 cagagtctgt tcaaaatgca cagccgatag aagatggtca aatgagagcg gtagttcgta
     1621 ttggtaatga tttttctggt aactattacg aggtgaaaat tccgctgaag atcactccat
     1681 ttggcacaac tgattcactg accatttggc ctgctgtaaa caatcttgat tttcaattag
     1741 agttactcac acagctaaaa tcaaggagaa ataaacaagc cgtgcctccg tcgcaatatt
     1801 attctgaaac ctctgctgat ggcagaacct atgctattct gggtaatccc aatcttggtg
     1861 aagtaagggg catgttgctg ggtgttgaaa acctggcgca ggaatccatg tgtacagaag
     1921 tctggttcaa tgagctccgt ttatctcaac tcgatgaaga gggtggatgg gctgcactgg
     1981 caagagcaga tattaaatta gcagatcttg gaaacatctc tttagcggca actgcgagaa
     2041 ccaggggttt tggaacactg gaacagcggg taaatgaaag aagcagggaa gatgtttata
     2101 cggtggatgc cactgctacc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn natgatcctt atgatttgga tattaaactg agagataaat tgaaagaggt
     2281 agatggtaag gaaagagact caataaaaag ggagtcactg gatgtaacaa ccacaaaaac
     2341 ttttaacctg accaacgtaa agaaaataaa gacagatggt aagcggccaa agatttggag
     2401 tgttaccaat ttcgatttca attattctta tatacaaaca ctcagctata acccgttgat
     2461 cgaaagagat gagatgagaa ggacccgagg tgcagtcgga tatacttatg ccccgcaagt
     2521 gcagcccttt gagccattca agaaactgat caagtcaaaa tcaaaatggt tggcattgct
     2581 gaaggaattc aatttaaatt atgcgccatc gcagatttct ttcagggcag atgtgttcag
     2641 gcagttcggc gccacccggc cgagaaatgt tggtggtggt ccgtacaaaa taccggagac
     2701 ctacaataag tactttacat tcgaccgttt ttatatcctg caatggaatc ttaccaaatc
     2761 aatatccatt gattttaatg ctgtcaataa tgccaggatt gatgagcctt ttggaaggat
     2821 tgataataaa gtagccaaag attctgttcg caaaaacttg ttaaagggcg gaagaaatac
     2881 cagctaccgt caggaagtaa ccattactta caatgttcca atgcagaaaa ttcctctgct
     2941 tgactggact acattaaggg ccagctataa taccaaatac aactggctgg caggttcatt
     3001 attggcaaga acacccgaaa ttgacttggg caatacgttg tccaataccc agaccaggac
     3061 catcaacggt gaaatgaact ttgaaatgtt gtacaataag tcccgtttcc tccgggcagt
     3121 aaataccagt gcgcctaagc agccacctgc caataatcag cctggtggag ataaaaatca
     3181 gccgggaggt aaaggtgata ataagaaaga ggataagaaa tcgaaaaata ataaaactaa
     3241 acaaggtgat aatgccggtg cacagcaaaa agatcagcag cagcaacaag ttgtacccgg
     3301 tatgccttct cagcaagata cttccaaagc aaaactgagc aaaaaagaaa gaagaaaact
     3361 ggcaaaactg gaaaggaagc agcaaagatt gcagaagaaa caacaacgaa agaaagatcc
     3421 caacaggccg cttccggaaa tttcaggtgt gccaaaggcc tttttacaat tggccacttc
     3481 tttaaagaga gtgggaattc aatataccga ggatatggga acaaccctgc ccggatatat
     3541 ggacagtacc agggtattag gttacaattt caagagccgt caacccggtt tcggttatat
     3601 attcggctac cagcccgata ccagttggat aaatcgtttc ggtgcaaaag gattactgtc
     3661 tgccgacacc ctggtgacag cgatgataca gcagcgctac aatcagcggt taaatcttac
     3721 tgcacaggta agtcctttcc gtgattttaa tattgatatc aattacgata aaacatttga
     3781 taagcagtat tcagaattgt ataaggatac cagtaagttt gataatgttg gattgacgag
     3841 attgaaccct tatgcactgg gaagttttaa tgttagctat atcgcattcc agaccctctt
     3901 taagaaattt gatccgaatg tagtatcaga aacctttcag aagtttgaga gcaaccgtgc
     3961 catactttca aagnnnnnnn nnnnnntaaa tccttaccag ggcggtgtga ttgaccctaa
     4021 cgatcctaat ttctataaag gatatggccg ctatgcacag gatgtggtaa tacccgcctt
     4081 tattgcagcc tacacgaata aggatcctaa tagcgtaagc ctgataaaaa atagcaaccc
     4141 aaatcttcgt tctaatcctt tctcgggaat taaagcaaaa ccaaactgga cagttaccta
     4201 tacaggcctt tcaagaatac cgggactgga taagatattt accaatgtat ctatccggca
     4261 tggataccac agcactttta gcatgaacag ctttaataca gcactgttgt tccttgatcc
     4321 gttcagggtg ggttatcctt cgttcacnnn nnnnnnnnnn nnnnnnnnna ttaaccggta
     4381 attttattcc ttacttcctg gtgccgaata ttactatcca ggaagcattt gatccgctga
     4441 ttgaaattga catgactttc actaaccagt tacagactcg ttttgagttc aggaagagcc
     4501 gcacactgag cctgagtctt atcgattacc agctggcaga gcaacgcagt actgaggtta
     4561 catttggatt taactggaga agaaaaggtc ttccgctgat caagaagctg cctttcatga
     4621 agggtgagat ggataatgat gtgactatcc gggttgactt cagtttgaga gatgatgcca
     4681 ctgccaacag caagcttgac cagcgaactg catttggtac tgccggtcag aaagtaatac
     4741 gtattgcacc ttctatagat tatgtggtca acaacagggt aagcatgaaa ctgttctttg
     4801 aacagaacag gaatattccc aagatcagta atgccttccc gattacaaat acaagggcag
     4861 gtttacaggt gagggtttca ttgacacagt aagaaataac ttaataggca atattgaaag
     4921 cggatgttgt gatcttcaga acatccgttt ttatttagtg gtatctctgg atataaaata
     4981 gatacagaag tgcagtggat aatcccactg acattttatt tgatcgttgc caattgaagt
     5041 agctttttcc aggataattt atctgaacct ggttttcggt gaattaaccg tagatattcg
     5101 ctgcctaaaa aaacgattac tattcaggct tatttggaga ggggcttaca ttgcccgtag
     5161 ctgcgtcgtt gctgcgtttg aacaacatta catagccaca aagatttttc ttcttttgct
     5221 tatttacctg taccggtttc atcatatgga actctgaaaa ctgtggtaca taggaatata
     5281 caataacatt accgtcaata tcaccccatt ttttctggcg gaatattacc attctttcct
     5341 gccagtcata catacgaact tcataaagcc gggatgaata atcaccaata aaaacgaact
     5401 ggtaccagct gccctgtgta agcggaacga tgaccggcat ttcaaattcg ctttccatat
     5461 taatggaggc ttctttcagc aaaacataac catcttttcc gtaaagattt ttcaggctgt
     5521 cggcctgttt gctgatcata tcattactgc aggattgaag acggatgaca tcctgtgaat
     5581 atccacaggc agtcataatg gtcatcatga ggatgaggag gtacggcttt ttcataacag
     5641 ggtttgtatg atattgggta gctgacataa agttagaaat caatttgctg tatcctgcat
     5701 tttcaaatca gttacattct aaaatgttcc tctgcggtta tatttgcgaa ggggttttac
     5761 ggcaagtatc gggccaacgg acggggataa agtttgaggt ctttaaagat agccgtaaaa
     5821 aagaagtttc ttcttttttt tgacctgtat caagaagact ggcggttgaa gtagatggcg
     5881 ttggcttgtt gcaggcaact tatggtcagg tcgttgatac aaacatgagc cggaagggta
     5941 gcagcataat agattacctc cgccacatct tctgcagtca atggttgtaa gccgtcatat
     6001 atttttttgg ctgttccttc atcaccttta aatcgtacaa cggagaattc cgtttccgca
     6061 gctccggggt tgatcgctgt tacttttata ttgtgcctta gcaaatcaat tcgcatggac
     6121 tggttcaatg catcaacagc atatttggta gcacaataaa catttccttt ttcatacact
     6181 tctttaccgg caacagatcc catattgatg atatggcctt gttttctggc cgccattaat
     6241 tgtgccacag ctttggcaac ataaagaaag cccttgatgt tggtgtcaat catcgtattc
     6301 cagtcatcga agcttgcttc ttcaaaatag tcacggccca atgccaggcc ggcattattg
     6361 atcagtacat ctatagcttt ccattcaagc gggatatttt caatagaact gatcgtatcc
     6421 gtctggtttt gcacatcaaa aggaagctgt agtacagcta cattgtattt gttttcgatt
     6481 tcatcggcta actgctgcag cctgtctgtt cttctgccat tgatgatcag atcatatccg
     6541 ttagcagcaa atttgtaagc acaggctttt ccaaatcctg atgtagcgcc ggtgatgaag
     6601 acgatttttg taggagaatt ttggtttata gttaatagtt cattgttcat agctggctac
     6661 ttacaagtaa ttgagctttg cagtaaggat accacaaact tagtaaacgt ctattaacta
     6721 tccaccatga actgtactac ctgatcaacg taaccgttcc tttcaggaaa acgggcttgc
     6781 ccaggtagtc aaccgcacta accatccata cataaacacc gcttgcctgc ggagtaccgt
     6841 tcactcttcc gtcccagccg gcacggttgg cagtggtgta gaatactctt tcgccccagc
     6901 gattgtagat gctgaagtag ttgatctttt gaatacctac tgcaattgga cgaatgacat
     6961 catttcggcc atcattgtta ggggtgaaag ctgtaggaac aaatacgtag ggatttgttt
     7021 taaacacagt tactgttaca aatgcagaat cgggacagtt cgcatcatcc caaacggtca
     7081 gtttatacct tacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     7141 gcaatattgg gatttttcag accggtatta ggagtccata aatatcttac gccacccgaa
     7201 ccagataact gtaaaggctg tcctacaatg actgtggtat ccctgcctgc gtaagccctc
     7261 actttgggct gtacggtgat gacaaccgta tcataaccgg gtttggggca gcccggtgta
     7321 tcatcccagg aagtaaggac aaaactagta gtccggggag gagaagctat tggatttaat
     7381 atgttcggat tgtttaaata gttagccggt gtccaggtaa aacgggtccc attatgtgca
     7441 ccgctcagtt gtgttgaagt gttataacat atcacttgtg gtgagccggc gttggcaacc
     7501 ggataaggat ctgtattgat cactacaaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ggttcagtat atttgggtta
     7621 ttgagtgttg ctcccggtgt ccaggtaaag tttagcccgt tggtaacagc atttagcgtc
     7681 actgcgtcgg taaggcaaat agtggtatcc ggcattgccg taagtgaaac ccccgatatt
     7741 actcttaccc ggacagaatc cttattcaca caaccattgt cggtaagatt ggccacatac
     7801 caggtatctg tagtggggtt gactgtaggg ttgggcgtat tggggttggt gatattagtc
     7861 agtggtgtcc agttaaaagt accagttccg ctggcactta aggtgactgc atcgggaaca
     7921 caaataaggg tgtcattaaa ggcaagggtg atcaatggtt tgtccagtac attgatagta
     7981 acaaaggccg tatcaataca gcctttgctg ttggtaacga tcaaccgtac atcccttgta
     8041 cctggactgg ggtaggtata ttgcgggttt cgtattcttg atgtatcggc aagtgtagtg
     8101 ccatcgccaa aatcccatcg ccagctatcc accaccccat aacgggtatt ggtggtatca
     8161 gtaaactggt aaggatttgt aaaacaactt ccgttataaa taaaaccggg aaagaagccg
     8221 ggatatactt ttactgttct gtaagcagaa tcagaacaac tggttcctcg atttattaca
     8281 aatttcaggg tataagttcc agtatctgtg tatgtgtagc ttgggttatt gatattcgat
     8341 gtgtcggcca aagtcgaaag atcaccaaaa tcccagaata cactattggc tcctgtactt
     8401 gtatgcgaaa attgaacatt aaatccatcn nnnnnnnnnn nnnnnnnnnn nnnnnntggt
     8461 gttgcagtgg taagctcaca atctgaaact tgtactataa ggtcttttct gtgagtggct
     8521 atctgcacgc cattcctgaa tacactgatg caaacactta ctacatactt tccgaaatca
     8581 ggcgccagtc ccgtaattac acctgtttga ggattaattg tcgctcctgt acctaaaggg
     8641 ttaatgccag taaaaggtga attgtagggt actgagttat aaggtggcgg ggcaggatcc
     8701 tgaaatcctg cgttaatagc ggctcctccg ttgtaggcgc tacacaaact ataagaaagt
     8761 gaatctccat caggatcggt tgcgctaaaa ttcagggtaa aaggtgcgtt gcggcaaatg
     8821 acatttacag gcaatgaaaa ctggggcgat gaatcattgc ctactggtaa ttgatttgat
     8881 cctggtatgc tgcatgcata agtagaccca gtactattac caacattgac catcccattt
     8941 atacgacaac aagtttgata tgcaacagtt tatccgtttg aattatttgg cagttcaact
     9001 accattgtat aaatggcata ggtataatta agtgttgggg cattttgaat acaggaagga
     9061 aatattatag gtactgatgg cgtacccggc ggatcttgag aaataagcca gttgttattg
     9121 gcagctgtac caattacttt ttgtccgtta tcgttattga aaatacctac tacgtaatta
     9181 ggggccaatg ccgcaccact tggatcatca cctctgaaaa gaaccattat gattctatag
     9241 ctttttgtgt taggtgctga acccgggccc aggtatacat accgcatttc accgccaata
     9301 atatgcgcag caaaagaagt attagacaaa aaaaggaaga caaatatggc tataacaagt
     9361 cgtctcatga ataggatcgg ccggtgttga tttttatgtt tccagcaaaa attcttccag
     9421 aatccggttg catttaccag gctcttccag catgcccata tgtccggatt gagaaaggat
     9481 gtgaatgtac gatttttcag gcaggtggca ctgcttcagg ctgtcttcca ttggtactgc
     9541 attatcgtat tttcccatga taaaaagcac aggcacaaca gctttacgca aaacattagt
     9601 ccggtcggga cgctgcatca tagcctggta atatgaaacg agtgaatggg gattaaagtt
     9661 gtttccctgt tccattaaat cttttattgt gtcagatagt ttatcttttg attccggagc
     9721 aaaaagatta ggtattaccg ttttcagaaa ttcaccggcg ccatgctgct gtataaaact
     9781 tattcctttt tgacgggtgg cctttttttc atcactgtca gcaaatgccg aggaatggaa
     9841 aagcccaaag gttgttaata cttcaggata tttttcagca agagctaacg ttatatatcc
     9901 gcccatgctg tgaccgatga cagggcaagg gacacatttt tcttcctgca tgatagcaag
     9961 gattacttca gccattccct ccatgctcat ttcatcagtt aattcagaac ggccacttcc
    10021 gggaagatcg ggaacgatta gtttgaattt atcctgcagg aactttacct ggttatgcca
    10081 gatttctccg tcttcgccaa agccatgcac taataacgcc ggcttaccgt tgccaatgac
    10141 acggtaaaaa atctcttttc ctttaatgga tatagtttta ttcatgttca gttgattgtt
    10201 cgctttctga tcttcaggaa aatagttgtc atggttagaa gtatgcttat tcctatcatc
    10261 agcttcgaca gtaaatcacc aaactttaca aagaatgtat ttgatttatt ctttgcggca
    10321 ctaatattat acttaatagc tgcgttagtg ttgtagggct gcggattgat tacttttcca
    10381 ttgggatcaa taaaacagct gatgccggta ttggcgctgc gggctaccca ggtacgggtt
    10441 tcaatggccc tcaatcttgc ataactcatg tgctgcttat gaccgggtgt tttcttccac
    10501 cagccgtcgt tggtaataat gcagatgaga ttggcgccat tgcggatata ccggctcatg
    10561 aattcaccat atatactttc ataacaaatt gccggggcta tcctataccc gttcttttct
    10621 tcaatcactg tacgatgatc ctgtttggca taaccggcag tggtgccgcc aaatttatcg
    10681 aaccatttgc tcaggaattt caggaaccag ggcagggttt ccacaccggg taccagcatg
    10741 gatttatggt aaaaaccatt ggcgccatta ctgtcaagta aaactgatcc gttgtatgat
    10801 tcgtaatgaa aaccattgta ttcttcggag tattttgtct tctcactaaa ctgacgatag
    10861 ctttcaatac cggtaaataa cgatgcttta ggatgttttt ttagaaagcc ccaaagaggg
    10921 ttcaggaaat agttgccttt gagattagcc tcatccattc ttgtcatcat atacaacgct
    10981 gtttccggcc aaacaattag agccgtatta tcatccgctg ctttttctgt tgtgctgatc
    11041 agcatttgca gttgtgcttc aaaggaaccg gcctcgctgg aaacttttac ataaggatca
    11101 atattgggtt gcactactac aatattctgg ttggtttgat ttcctttctc tatccggaag
    11161 gttgttattt tagataccag tatgggcaac accagcaaca gtgcagccgg aatgaggaag
    11221 cggtaatctc ttgtcctgtt taccagcata ttttttttca aatgcaggaa caaaagaata
    11281 ttgacgccca gcacccataa agtgccgccg ctggtgccgg tatattcata ccactgcacc
    11341 cattctgttt tggtggcaaa tgcattaccc aatgtaagcc agggccagct aagcccccag
    11401 tcctggaggt gaatatattc aaagcac
//
LOCUS       DAORLS010000073        11332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3064_length_11332_cov_2.206704, whole genome shotgun sequence.
ACCESSION   DAORLS010000073 DAORLS010000000
VERSION     DAORLS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11332
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_11332_cov_2.206704"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>354
                     /locus_tag="PK977_07340"
     CDS             <1..>354
                     /locus_tag="PK977_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRF17964.1"
                     /translation="LLDKNINIDAIISSPAKRAAKTAKAFAESYDLKKSRLQFKEELY
                     MAGSAAFTAVIESADEKFESIAVFSHNPGITDFANELTNARIDNIPTCGVFAIKINTD
                     TWKNFRNAPKEFWFF"
     assembly_gap    355..364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(386..811)
                     /locus_tag="PK977_07345"
     CDS             complement(386..811)
                     /locus_tag="PK977_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17965.1"
                     /translation="MKNLQTALAALCISTTLLFTSCSPDYDQVSGTEEVLTRNAWEID
                     HFSRSSQNLTSEYTNNTLYFSNNGTLICRNGADQCTGTWSYSGNTMGETIIIQLNSND
                     TKMLQLNHAWQLRSRNLSNILLEQTDPAVNSELRIRVTQ"
     gene            complement(939..1829)
                     /locus_tag="PK977_07350"
     CDS             complement(939..1829)
                     /locus_tag="PK977_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF17966.1"
                     /translation="MNTSLKAHIAVLFTNIFFAANFSLVKYISPALVGPYGLNIIRVG
                     VSLCLFWLVWSFGKTGASIQKKHIPRFIICGLTGVAINQMLFIKGLTMTSTIHASMLM
                     LCTPLLITLFAFWILKESVTIIKVAGLLLGIGGAALLIMNKESTGTASLTGDLLIIMN
                     AISYAFYFILVKPLMVIYHPLHIIRWVFTIGFIMILPFGWSQFDAINWSVFTLQDVAA
                     LLMIVFCGTFLAYSFNIYGIRHLGAGTTGSYIYTQPVFAALVAVFFLGEEMTIEKGLA
                     GLLIFLGVFLVSRKQLAKTP"
     gene            complement(1870..3036)
                     /locus_tag="PK977_07355"
     CDS             complement(1870..3036)
                     /locus_tag="PK977_07355"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF17967.1"
                     /translation="MLLSQNKQKINPDQTETEKLLGKTDTTGRKSRILVAPLDWGLGH
                     ATRCIPIIRELLQQGAEVWLAGEGAQEHLLRLEFPDLPFLQLKGYRVKYASTRSGVIR
                     KIIMQSPRFLKAVKREQEWLKKTVKENNIDAVISDNRYGLYHTEIPCIFITHQLAIKS
                     PLGKWSEQLLQKKNYKYINRFTECWVPDLPGDDNLAGDLSHPAIIPKVPVKYIGWLSR
                     FSKRTLQEKAGHLLIVLSGPEPQRSLFETTVIRDIARYEGSVTIVRGLPGTHTLLPST
                     NSIRIYNHLPAKQLNEEMEVAEFVISRSGYSTVMDIAALQKKSILVPTPGQTEQEYLA
                     GYYMEKGIACAALQQFFSLPEELQKARLIPYRIPAFSYSNNLKTTIQDLLSRLR"
     gene            3255..3497
                     /locus_tag="PK977_07360"
     CDS             3255..3497
                     /locus_tag="PK977_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17968.1"
                     /translation="MIFSNRDILVASRHVGMDEQILEKEIEILHSLLQQTETPESFCR
                     AHELVNRNRITSKASLILKETRYHRLKPFRFLINKN"
     gene            complement(3509..4765)
                     /locus_tag="PK977_07365"
     CDS             complement(3509..4765)
                     /locus_tag="PK977_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF17969.1"
                     /translation="MKLSHLSETLIGSEIVKLGGEIREKIRQGERIYNFTIGDFDPSI
                     FPIPKELEDAIVEAYRKHFTNSPAAEGNLDLREAIHSFMKDEEGLDYSINEILVASGG
                     RPLIYTVFRAICDIGDKIIYAVPSWNNNHYTHFVGGEHIVIEAKAKNNFMPTASDLKP
                     HISDAVLISLCSPQNPTGTTFRKEELEAICDMVIEENKRRADGQKKLYVMFDQMYWHL
                     TYGEIKHYNPVSLRPEMREYTIFIDAISKVFAATGVRVGWGMGPSAVISKMKAILTHI
                     GAWAPMAEQKAVAYYLGNREAIHKYLANFKSEIEERLRRIYNGFAGLKKEGHPVDAIA
                     PEAAIYLTIKIDLVGKTSADGKLLATQSDVTAYMLNNAGLAVVPFYAFGAEKNSPWYR
                     LSIGTCKKEEIDEMIGKLREALISLK"
     gene            complement(4884..5366)
                     /locus_tag="PK977_07370"
     CDS             complement(4884..5366)
                     /locus_tag="PK977_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17970.1"
                     /translation="MKKIIAAGILFFLITTSRYADAQVRVNLNINIGSQPAWGPVGYD
                     YVEYYYMPDIEAYYYVPGQQFVFFSNGRWVFSAYLPAVYRDYNLYSAYKVIINQPRAY
                     QYYNTHKVKYAGFRGKGNTQIIIRNSNDPKYYVVKGHPKHAHKSPGRNNGKGRGRGQH
                     "
     gene            complement(5471..6253)
                     /locus_tag="PK977_07375"
     CDS             complement(5471..6253)
                     /locus_tag="PK977_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17971.1"
                     /translation="MMKMNTGAWIGIIGGAIGFLAAIAAVIVTTGSAGIYITIGMLAL
                     FGGMGFLFYKLFFAPMINANRLQKTGLPGTAKILEVRDTGVTINNNPQIKLIVEIKSS
                     LGQRYTTTIKTLVSRINPFAYQPNMEVPVKIDPQNEQNVVLDYSQNRPATANNMFTRQ
                     NTDALKIELEQMQKENEAILMSGRSARAIIKKYTWLGVNVNGNNPYVELEIEVLPEAS
                     ASFSSKVKGVIAELSVEKYQPGKEIYVKYDLYDNSKVAIDHS"
     gene            complement(6327..7349)
                     /locus_tag="PK977_07380"
     CDS             complement(6327..7349)
                     /locus_tag="PK977_07380"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRF17972.1"
                     /translation="MKTMRLLFFFILAVIVVTAALSFLLPTSQKVERSEVINAPAKTI
                     YQQLIKLENFNKFSVWSQQDSSAVYTINGTDGTVGAFSSWTGSPEISGEGKIEIIALE
                     QDRKVEQKLHFTQPKEGDASSVFTLNENNGLTTITWSFEMTTPRPWNIFNLFYSMDKQ
                     MGKDFEQGLATLKSLIEKQAGTAGNNNYEVLTMDFPSTTFAVIRQQVKWSDIYTFQTQ
                     HMPIIRNEAIKANASPGTAASLFYKWDEKNQLTDMAAALPVINGTELNNSLVKTENIS
                     TGKAVYVNHYGNYEKAINAFNTIYAYLDNHKLKQKFPVIQQYITGPASEKDTAKWLTK
                     IVFLVE"
     gene            complement(7349..>8164)
                     /locus_tag="PK977_07385"
     CDS             complement(7349..>8164)
                     /locus_tag="PK977_07385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF17973.1"
                     /translation="PRAGGVPKPEDLDKISLDASYRIFKERFANAGDFTFVFVGSFDE
                     DSIKPLLEKYIGSLPGKPQKESFRDLGIRNPSGKIDKTVNKGADQKSFVTMVFNQPAT
                     YSSKDAYALSCLGEVMTIKLVEQLREEKGGVYGVSANGSMQRIPVTTSMFTISFPCAP
                     ENVDTLSKAAIDELKKIISNGVSTADLDKVREQQKRKLETDLKQNIFWLSNLADAYYY
                     GNNPADILNKQKMAEYLTSKMIQDAAKKYINLDKYIRMVLKPEATEEKTKKAF"
     assembly_gap    8165..8186
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8187..>9121)
                     /locus_tag="PK977_07390"
     CDS             complement(<8187..>9121)
                     /locus_tag="PK977_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17974.1"
                     /translation="RSLYALMEEGRRVLLYGFTPAEFELQKKQVESYYDRIFNEREKE
                     ESYKYVDEYVNNFLINEPIPGIEWEYDFVKQYLASVKLEEVNSLAKQWITPVNMVVTL
                     NAPEKEGIKIPSAEEVKAVLASVETAKIEPIKEKALATALMDAGKLKPGKTISSKTDD
                     NLETTTLKLSNGATVILKSTNFKNDEILFRAFSKGGHSTVKDADYYSGLYASQIAMQS
                     GVSNFSAIELGNMLKGKNTSVSANISLYGEGMNGGTIPKETETLLQLIHLYFTQPRKD
                     KEAFESFKTRQKQLFGNLMANPQIFFSNEFQKLMT"
     assembly_gap    9122..9219
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9220..>9830)
                     /locus_tag="PK977_07395"
     CDS             complement(<9220..>9830)
                     /locus_tag="PK977_07395"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF17975.1"
                     /translation="PTDKPELVKKGLQILEDWAHNATLDSLEIEKERGVVIEEWRLSR
                     GADERMMKQTLPVQYRGSKYADRLPIGTLESLQSFSHAALKRFYKEWYRPDLQAIVVV
                     GDIDVNEMEQKIKEIFGDIPPPVSPRKRESFPVPDHTETLTVVAKDKETAFPSLEVMF
                     KKDPQPETTIGDYVRYMNRLLFTGMLNSRFRETTLKPNPPFVSA"
     assembly_gap    9831..9920
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9921..10241)
                     /locus_tag="PK977_07400"
     CDS             complement(<9921..10241)
                     /locus_tag="PK977_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF17976.1"
                     /translation="MQAVKKCWLFVLVFLLLANFSWSQINREQPMPVDPKVKIGRLSN
                     GMTYYIRHNSKPEQRVELRLVVNTGSVMEDEDQLGLAHFMEHMNFNGTKRFPKNELVN
                     YLQSI"
     gene            complement(10341..10916)
                     /gene="msrB"
                     /locus_tag="PK977_07405"
     CDS             complement(10341..10916)
                     /gene="msrB"
                     /locus_tag="PK977_07405"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRF17977.1"
                     /translation="MKVSIKYLFVLLTAGLLQQCSYSQDNTTRKNETTMDSTKKNNPV
                     YSNTDTGKVVMSEEEWKKVLPADVFYIARLKGTERPWTSKFESFKEVGTYYCAACGNA
                     LFKSDTKFDSGCGWPSFYEPINKGSIIYTPDNSHGMQRTEVQCGRCKAHLGHVFDDGP
                     PPTGLRYCINGVILDFKKAQEAEKNYQDKQQ"
     gene            11090..>11332
                     /locus_tag="PK977_07410"
     CDS             11090..>11332
                     /locus_tag="PK977_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRF17978.1"
                     /translation="MFNLVLFGPPGSGKGTQSDKLVEKYKLVHLSTGNLLREEIAHHT
                     PLGMEAKSFMDKGQLVPDEVVIGMVDNYFDKHKGARG"
BASE COUNT         3007 a         2274 c         2281 g         3550 t
ORIGIN      
        1 ggctgctgga taagaatata aatatagatg caattatctc aagccctgcg aaacgggctg
       61 ctaaaaccgc caaagctttt gcggaatcat atgatctaaa aaaaagcagg ctgcagttta
      121 aggaggagct ttacatggca ggttcagccg cttttactgc tgttattgaa agtgcagatg
      181 aaaaatttga gagtattgct gttttttctc acaatcccgg tattaccgac tttgccaacg
      241 aattgacaaa tgcccgtatt gataatattc caacatgcgg ggtgtttgca ataaaaatca
      301 atacggacac ctggaaaaat ttccggaatg cacccaaaga attttggttc ttcgnnnnnn
      361 nnnntatccg aaagctacag gataattact gcgttaccct gattcgtaat tcgctgttta
      421 cggccggatc agtttgttcc agcaatatgt tagagagatt tcttgacctt aattgccagg
      481 catggttcaa ctgcagcatt ttagtgtcat tgctgttaag ttgaatgatg atggtttctc
      541 ccatcgtatt gcctgaataa ctccatgtac ctgtacattg atcagcccca ttgcgacaga
      601 taagcgtacc gttattactg aagtacaatg tattgttagt gtattcgctt gtcaggttct
      661 gtgatgaacg ggaaaaatgg tcaatctccc aggcattgcg ggttaatact tcttcggtac
      721 cagaaacctg gtcataatcg ggtgaacagc tggtaaaaag cagtgtagtg cttatgcaca
      781 acgctgcaag ggcagtttgt agatttttca ttctgattgg attttggtat tcgaaaataa
      841 aaaatcagta ttgcttgtca atgaaccata acacccaatt acaaaaattg gagtattagt
      901 acttttccct taatcgtagt tttccgattt tgccggtatt aaggggtttt agccagctgt
      961 ttacgactta cgagaaagac ccccagaaaa atcagcaggc ctgccagccc cttttcaatg
     1021 gtcatttctt cgcccaaaaa aaatactgct accagggctg caaaaacagg ttgtgtataa
     1081 atataagagc cggtagtgcc tgcaccgaga tgccggatac cataaatatt gaaactgtaa
     1141 gctagaaacg ttccgcagaa aacaatcatt agcaatgctg ctacatcctg caaagtaaag
     1201 acggaccagt tgatagcatc aaattgtgac catccaaagg gcaatatcat aataaacccg
     1261 attgtgaaca cccagcgaat gatgtgcaaa ggatgatata ttaccatcaa tggctttacc
     1321 aggataaagt aaaaggcgta ggaaatggca ttcatgatga tcagcaaatc accggttagt
     1381 gaggcagtac ctgttgattc tttgttcatg ataagcaggg cagcgccacc aatacccagt
     1441 aacaggcctg ctacttttat aatagttaca gattctttta aaatccaaaa agcaaaaagt
     1501 gtgatcaata aaggggtgca tagcatcagc atagaggcgt gaatggtaga ggtcattgtg
     1561 agtcctttaa taaaaagcat ctggttgatg gccacaccgg ttaacccgca gatgatgaaa
     1621 cgaggtatgt gttttttttg aatactggct cccgtttttc caaaagacca aacaagccaa
     1681 aacaggcaaa gactgacacc tacccgaata atatttaatc cgtaaggacc taccaatgcc
     1741 ggtgaaatat attttacaag actaaaattg gcagcaaaaa atatattggt aaataaaaca
     1801 gctatatgcg ctttcaggct ggtgttcaat gcaaaaactt taagagagtc aaagttaacc
     1861 gcaaaaatat tagcgaagcc gtgataacag atcttgaata gttgttttca aattgttgct
     1921 gtaagagaat gcaggtatcc ggtagggtat gagcctggct ttttgtaatt cttcaggtaa
     1981 ggaaaaaaat tgttgtaaag cagcacaggc gatacctttt tccatgtaat agcccgcaag
     2041 atattcctgt tcagtttgtc ctggtgttgg cacaaggatg cttttctttt gtaaagcggc
     2101 tatatccata acagtactgt acccgctgcg gctgatgaca aattccgcta cttccatttc
     2161 ttcatttagt tgttttgccg gcaaatgatt gtatatcctg atagaattag tagacggaag
     2221 caatgtatgg gttccgggta atccccttac aatagtaacg ctgccttcat agcgggcaat
     2281 atctcttatt acagtggtct caaataaact gcgttgcggt tcgggaccgg aaagaactat
     2341 tagtaaatgt cctgcttttt cttgcaatgt ccttttgcta aatcttgaaa gccagcctat
     2401 atatttgacc ggtactttag gtattattgc cggatgtgat agatcgcctg ccaggttatc
     2461 atctcccggc agatcaggta cccagcattc agtaaagcgg ttgatgtatt tgtagttttt
     2521 tttctgtagc agttgttcgc tccatttccc taaaggactt tttatggcta actggtgggt
     2581 gatgaatatg cagggtattt cagtatgata taagccatac cggttatcac taataactgc
     2641 atcaatatta ttttctttga cagtcttttt cagccattcc tgttcccttt ttacagcttt
     2701 gagaaacctg ggcgattgca taatgatctt tctgatcacc ccagatctgg tagaagcata
     2761 ttttactctg tatcccttaa gctgcaaaaa gggcaggtcg ggaaattcta aacgaagcaa
     2821 atgttcttgt gcgccttcgc cggccagcca aacctctgcg ccttgctgta atagttcccg
     2881 gataatggga atgcagcggg tggcatggcc aagtccccag tccagcgggg ctaccaaaat
     2941 gcgggatttt ctaccggttg tatcggtttt gcccagtagt ttttccgttt ctgtctgatc
     3001 agggttaatt ttttgcttgt tttggctcaa cagcacacta atttgtttgt aaaatagttt
     3061 taatttagaa tactgaagat atgaggaaca tcaacaaatt agccttaatc gtgttaatta
     3121 ttagcacagt tttgtttact gcctgtaacc gtaattatta ttccggtagc ggaaaaggcg
     3181 gcaaaaattg tggctgtcct agcacaaaag gcatgacagg ctattgatag tgtaaagaaa
     3241 caaggccaac aagaatgatt ttttcgaatc gtgacatact ggttgcatca cgccatgtag
     3301 gtatggatga gcaaattctt gaaaaggaga tagagattct tcattcactg cttcagcaaa
     3361 cagaaacgcc tgaatcattt tgcagggcgc atgagttggt aaaccgtaac aggattactt
     3421 ccaaagcctc ccttatttta aaagaaaccc ggtatcaccg gttgaaaccc tttcggtttt
     3481 taatcaataa aaactgataa gggggatttt acttcaggct tatcagtgct tctcttaatt
     3541 taccaatcat ctcatcgatc tcttcttttt tgcaggtacc aatacttagt ctgtaccagg
     3601 gagaattttt ttctgcgcca aacgcataaa aaggtacaac tgccaaaccc gcattgttta
     3661 acatgtatgc tgttacatcg ctttgggtgg ctaataattt accatctgca cttgtttttc
     3721 ccactaaatc aatctttatt gtgagataaa tagccgcttc aggagcaatt gcatcaaccg
     3781 ggtgtccttc tttttttaaa ccggcgaatc cattgtatat gcgtcgtaat ctctcctcta
     3841 tttctgattt gaaatttgcg aggtatttgt gaatagcttc cctgtttccc aggtaatagg
     3901 caaccgcttt ttgttcagcc atgggggccc aggcaccaat atgcgtaagg atagctttca
     3961 ttttactgat gacagccgaa ggtcccatgc cccagccaac ccttacgcca gtagcagcaa
     4021 aaactttact gattgcatca atgaaaatag tgtattcccg catctctggt cttaacgaaa
     4081 cggggttgta gtgtttgatt tctccatatg tcagatgcca gtacatctgg tcaaacatca
     4141 catacaactt tttctgccca tcagctcttc gtttattttc ttcgatcacc atatcacaaa
     4201 tggcttccaa ttcttctttg cggaaggtgg tacccgttgg gttttgtggt gagcaaagag
     4261 aaatcagcac tgcatcactg atatggggtt tcaggtcact ggcagttggc atgaagttgt
     4321 ttttagcttt ggcttcgatg acaatatgtt cgcctcctac aaaatgtgtg taatggttgt
     4381 tgttccagct tggaacggca tatatgatct tatcacctat atcacaaatg gcacggaata
     4441 ctgtgtagat cagtggccgt ccgccggaag ccaccagtat ttcattgata ctgtagtcaa
     4501 gcccttcttc atctttcatg aaagaatgaa tggcttccct caggtcaaga ttaccttcag
     4561 cggcggggga attggtaaaa tgttttcgat aagcttcaac aatagcatct tccagttctt
     4621 taggaatagg aaaaatagag gggtcaaaat caccgatagt aaagttgtaa atacgttcac
     4681 cctgtcggat cttttcccga atttctccac ccagttttac gatttcagat ccgatcaatg
     4741 tttcggataa atgtgaaagc ttcataaccc catcctttga agcaaaaata atgggaatgc
     4801 cggagatggc aagacaaaga cctgtttagc ttaattagaa gaaagttaga aaatggccgt
     4861 aaagacagtc attttcactt tgtttaatgt tgaccccttc ctctgccctt tccattattt
     4921 cttccaggtg atttgtgagc atgtttggga tgtcccttta ctacatagta tttgggatca
     4981 ttgctattcc tgataatgat ttgcgtattc cctttccccc tgaaacctgc atatttcact
     5041 ttatgtgtat tataatattg ataagctctc ggctggttaa taattacttt ataagcggaa
     5101 tagagattat aatctctgta cactgcaggc agataagcgg agaataccca tctgccatta
     5161 gaaaaaaata caaactgttg cccgggaaca taataatagg cttcaatatc cggcatataa
     5221 tagtactcta catagtcata tcccacaggt ccccatgcag gttgtgaacc gatgttgatg
     5281 ttcaggttta ccctaacctg tgcatcagca tacctggagg ttgttattaa gaaaaaaagt
     5341 atgccagctg caataatctt tttcatacgc ttcgattttg tttcactgaa ttttttcaaa
     5401 gacagtgcca aagcgtaaaa aagaaaatca aaagaatctt atgctctcgt tttcagtaac
     5461 taagaaaaaa tcaggaatga tcaattgcta ctttagaatt atcatagaga tcatatttaa
     5521 catatatctc tttaccgggt tgatactttt caacagataa ttcggcgata acgcctttaa
     5581 ccttactgct gaaagatgca gaagcttcag gtagtacttc tatttccagt tctacgtagg
     5641 gattattgcc atttacattt acacccagcc aggtatattt ttttataatg gctctggcag
     5701 atcggccgga cattaatata gcttcatttt ctttttgcat ttgctccagt tcgattttca
     5761 atgcatctgt attttgccgg gtaaacatat tatttgcagt tgctggccga ttttggctat
     5821 aatcgagaac tacattttgt tcattttggg gatcaatttt tacaggtact tccatgtttg
     5881 gctgataagc aaaagggttg atccttgata ccagtgtttt aatagttgta gtgtaacgct
     5941 gaccgagact actctttatc tctacgatta attttatctg cggattattg ttgatggtga
     6001 caccagtatc tcttacttcc aggatctttg ccgttccggg taatcctgtt ttttgaagcc
     6061 ggtttgcatt gatcatcggg gcgaagaaaa gtttgtaaaa aagaaagccc attcctccga
     6121 aaagtgccag catgcctatt gtaatgtaaa tgcctgctga acctgttgta acaatgacag
     6181 cagcaatggc agctaaaaac ccgatggcac ctccgataat gccgatccag gcgccggtat
     6241 tcattttcat catagaatag ttttaattgg tgcaacaagt taataaaaat gagttactca
     6301 ttgcggccaa tttgtatgat gaaattttat tcaacaagga agacaatctt ggtcagccat
     6361 ttagctgtat ccttttccga ggcagggccg gtaatatatt gctgaatgac gggaaatttt
     6421 tgtttcagtt tatgattatc caggtaagca taaatagtgt tgaaagcatt aattgctttt
     6481 tcataattgc cataatgatt tacataaact gctttaccag tggatatatt ttcagttttt
     6541 actaaagaat tattgagctc tgttccattt attactggca gggcagcagc catatctgtt
     6601 aattgatttt tttcatccca tttgtaaaat aaactggcag ctgttcccgg agaagcattt
     6661 gccttaatcg cctcgttgcg gatgatgggc atgtgctgcg tttgaaatgt atagatatca
     6721 ctccatttta cctgttgccg gataacggca aatgttgtgg aaggaaaatc cattgtcagt
     6781 acttcgtagt tattatttcc agctgtgcct gcctgttttt ctattaagga ttttaaagta
     6841 gcaagaccct gttcaaaatc ctttcccatt tgcttatcca tactgtaaaa caggttgaaa
     6901 atattccatg gccggggtgt ggtcatctca aaactccagg tgatggtagt aagcccgttg
     6961 ttctcattta aagtaaaaac agagctggca tcaccttctt ttggctgcgt gaaatgaagt
     7021 ttctgttcca cttttctgtc ctgttccaat gcaatgattt caatctttcc ttcgccggaa
     7081 atttcagggc taccggtcca gctactgaat gcacctactg taccgtctgt accgtttatt
     7141 gtgtaaacgg cagaagagtc ctgctgtgac cagacagaga atttattgaa gttttccagt
     7201 tttataagtt gctggtaaat ggttttggcc ggtgcattga tcacttcaga ccgctctact
     7261 ttctgtgaag tgggcagcag aaaagataag gcagctgtta caactattac tgccaaaata
     7321 aagaaaaaca aaagcctcat cgttttcatc aaaatgcctt tttagtcttt tcttctgttg
     7381 cttccggttt taataccatg cggatatact tatcaagatt gatatacttt ttggcagcat
     7441 cctgtatcat tttggaagta agatattcag ccatcttttg tttattcagg atatcagcgg
     7501 ggttgttacc ataatagtat gcgtcagcaa gattactgag ccagaaaata ttctgtttta
     7561 aatctgtttc cagctttctc ttctgttgct ccctgacctt atccaggtca gcagtactaa
     7621 cgccattgct gatgatcttt tttaattcat caatagcagc tttgcttaat gtatcaacat
     7681 tttccggcgc acaggggaat gaaatagtga acatggaagt tgtaaccggt atccgttgca
     7741 tactgccatt ggcactcaca ccatatacgc cgcctttttc ttcccgtagt tgctctacca
     7801 gtttgatggt cattacttca cccaaacagc ttaaggcata ggcatctttg ctgctgtagg
     7861 tggccggttg gttgaaaacc attgtcacaa agcttttctg atctgcgcct ttattcactg
     7921 tcttgtctat tttgcctgaa gggtttcgta tgccgaggtc acggaaagat tctttttgtg
     7981 gttttcccgg gaggctgcca atatattttt ccagtaatgg ttttatgctg tcctcatcaa
     8041 acgatcctac aaatacaaac gtaaaatctc cggcattggc aaatcgttct ttaaagatcc
     8101 ggtagcttgc atcaaggctg atcttatcca gatcttccgg tttgggtaca ccacctgctc
     8161 ttggnnnnnn nnnnnnnnnn nnnnnnttgt cattagtttc tgaaactcgt tgctaaaaaa
     8221 gatttgtggg ttagccatca aattgccaaa taattgcttt tgcctggttt taaatgattc
     8281 aaatgcttct ttgtctttgc ggggttgtgt aaaatagaga tgaattagtt gaagcaatgt
     8341 ctctgtctct ttaggaatgg taccgccgtt cattccttct ccgtataaag aaatatttgc
     8401 agaaacgctg gtgttttttc ctttcagcat attgcccagc tcaattgcgg aaaagttgct
     8461 tacgccgctt tgcatagcaa tctgtgatgc atataacccg gaataataat cagcatcttt
     8521 tacagttgag tggcctcctt tactaaaggc cctgaacagt atttcatcat tcttaaagtt
     8581 ggttgattta agaattaccg tagcgccgtt agaaagtttg agtgtcgtag tttccaggtt
     8641 atcatctgtt ttactgctga tagttttgcc tggttttagt ttgccagcat ccatcagggc
     8701 agtggccagt gctttctctt ttatgggttc aattttggca gtctccactg atgcgagcac
     8761 agcctttact tcttctgccg aaggtatttt aatgccctct ttttccgggg catttaatgt
     8821 taccaccata tttacaggag tgatccattg tttggcaagg ctgtttactt cttccagctt
     8881 tacagatgcg aggtattgct ttacaaaatc atattcccat tcaattccgg gtatgggttc
     8941 attgataagg aaattattga cgtattcatc tacgtactta taggactctt ctttttctct
     9001 ttcattgaaa attctgtcat aatagctctc cacctgtttt ttctgtaatt caaactctgc
     9061 cggtgtaaag ccatacaata ataccctgcg gccttcttcc atcagtgcat acagggatct
     9121 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggaaacaaa tggcgggtta
     9241 ggttttaatg ttgtttccct gaaacggcta tttagcatac cggtaaaaag cagccggttc
     9301 atatagcgta cataatcacc aatagtggtt tccggctgcg gatctttttt gaacatcact
     9361 tccaaagacg ggaaagctgt ttccttgtct ttggctacca cggtcagggt ttcagtgtga
     9421 tccggtacgg gaaaggattc tctttttcgg ggagaaacgg gaggcggtat atcaccaaat
     9481 atttctttga tcttttgttc catctcattt acatcaatat cgcctaccac tacaatcgcc
     9541 tgcaggtccg gccggtacca ttctttataa aatcttttta gggcagcatg tgaaaaggat
     9601 tgaagagatt ccagcgttcc gatgggcaaa cggtcagcat attttgaacc cctgtattgt
     9661 acgggcaagg tttgcttcat cattcgttca tcggcgccac ggctgagccg ccattcttct
     9721 attaccaccc ctctttcttt ttctatttca agggaatcca gggtggcatt atgcgcccag
     9781 tcttccagta tttgcagacc tttttttacc agttctggtt tatctgttgg nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn gatgctttgc aggtaattta ccagttcgtt ttttggaaag
     9961 cgtttggtgc cattaaagtt catatgctcc atgaagtggg ctaatcccag ctgatcttca
    10021 tcttccatta cagagccggt gtttaccacg agtcgcagtt ccactctttg ttccggcttg
    10081 ctgttgtgcc ggatataata agtcatacca ttggacagcc tgccaatttt cactttgggg
    10141 tctaccggca taggctgttc cctgttgatc tgcgaccatg aaaaattggc taaaagcaaa
    10201 aaaacaagta cgaatagcca gcactttttt accgcttgca taagtatctt ttttgggaca
    10261 gtaaatttat cggaatagaa ggggttggaa aaataaaaaa cccggcttaa ccgggtttct
    10321 catttattac ttaatagtac ttattgttgt ttgtcctgat aattcttttc cgcctcctgt
    10381 gcttttttaa aatcgagtat cacaccattt atacaatagc gaagcccagt cggtggaggg
    10441 ccgtcatcaa atacatggcc caggtgagct ttgcaacggc cgcattgcac ttctgttcgt
    10501 tgcatgccat ggctgttatc gggagtataa atgatgcttc ctttattgat cggttcgtaa
    10561 aaactgggcc atccgcagcc gctgtcaaat ttggtatcac ttttaaataa agcattgccg
    10621 caggcagcac agtaataggt gcctacttct ttaaaactct cgaacttact ggtccatggc
    10681 ctttcggtcc ctttcagccg ggctatataa aatacatctg caggcaatac ttttttccat
    10741 tcttcttcgc tcattaccac tttaccggta tctgtattgg agtaaaccgg attgtttttt
    10801 ttagtgctgt ccatggtcgt ttcgtttttt cgggttgtat tatcctggga ataactgcat
    10861 tgttgcaaca ggcctgcagt taacaggaca aacaagtatt ttatagatac tttcatatta
    10921 tgctttaaca aatgtacggc aacatagttt gcatggtttt cggctgattt tataatggca
    10981 aaccttaaca ttttttagat atatacagga atatggcaat tctgaccggg aacaggcgga
    11041 atgcaagccc tgtacattat atttgcccat cacccaatgc taattgttta tgttcaacct
    11101 agtattattc ggccccccgg gaagtggcaa aggaacccag tcagacaagc tggtagagaa
    11161 atataaatta gtccatcttt ctaccggcaa cctgttgcgg gaggaaatag cccaccatac
    11221 tccgctggga atggaagcca aaagttttat ggataaaggc cagttggtgc ccgatgaagt
    11281 ggtgattgga atggtagata attattttga caagcacaaa ggagccagag gt
//
LOCUS       DAORLS010000074        11183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3123_length_11183_cov_1.785316, whole genome shotgun sequence.
ACCESSION   DAORLS010000074 DAORLS010000000
VERSION     DAORLS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_11183_cov_1.785316"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1084
                     /locus_tag="PK977_07415"
     CDS             <1..>1084
                     /locus_tag="PK977_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17979.1"
                     /translation="VYVHVGVITNLSTSSSDWRYVRFTWGTTTAAAQATSLGSNQWQY
                     TINNPRTYFSVPAGETILKIAILFRSGNGNLKQANTDGSDMYIPVYTTTIATKFSVPP
                     FQPTFIPIPEPISKQQNDFINLTAIANNTAENMKLYLNGTQIASANNVNTISANPQLT
                     VAGNQTIVAEATLTGTTRTDTLRFFVASAPVVAPLPAGVRDGINYEPGNTSVVLVLNA
                     PGKNRVSVIGDFPGSNWIEQTNYVMNKTPDGNHWWLRITGLTPGQEYSFQYLVDGTLR
                     VGDPYAEKILDPFNDGFITAATYPGIKPYPTGLTTGNVSILQTNAPAYNWTVTNFNRP
                     DKRNLVIYEMLLRDFVAAHDWKTIRDT"
     assembly_gap    1085..1094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1095..2729
                     /locus_tag="PK977_07420"
     CDS             <1095..2729
                     /locus_tag="PK977_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF17980.1"
                     /translation="SYLQRLGVNAIQLMPFNEFDGNESWGYNPAYFLAPDKYYGPKNS
                     LKEFIDTCHRRGIAVIMDIALNHTTGASPLAALYWNSSTNQPAANNPWLNITATHPFS
                     VFNDFNHESLRTRYFTSRVIEHWLQEYKLDGFRWDLSKGFTQTNSGSNVGLWGQYDAS
                     RVAIWKRYYDTMQLKSPGTYCILEHFADNSEEIELSNYGMLLWGNNTAQWQEATMGYT
                     NTSNFEGNIHSVRNWSNPHLIGYMESHDEERLMYKNLQFGNTAGTYNTKDLNTALKRM
                     EQAAAFFFNIPGPKMMWQFGELGYDYSINHCPDGTINNNCRTANKPIRWDYKNVIQRQ
                     RLYDIYSAMNKLRFHPWYKDIYIANAVSIDRSLAGGFKFLRLRSANDTSCVVVVGNFD
                     VVQQSGTVTFPVAGTWYDYLNGGTFTATGTAQSFTLQPGEYRVLLNRNLTNAVVTSTG
                     GSPTVSAAGLTASLFPNPANSNSVLEINVPETGKVQVELYNQLGQQIKTVFSDRLLKG
                     NHRIPLTGKVDNLPAGSYLLTIQQAGVKVSVKMIIQ"
     gene            2750..>3042
                     /locus_tag="PK977_07425"
     CDS             2750..>3042
                     /locus_tag="PK977_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF17981.1"
                     /translation="MSKALNLKTAEIEKISHLDYFNIAISVDCVIFGYDDKDLKVLLI
                     KSDLEEFEGLYSLLGDLVRPDEDLETASYRILKERTGLDDVYLEQVQAFGSLD"
     assembly_gap    3043..3072
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3073..>3359
                     /locus_tag="PK977_07430"
     CDS             <3073..>3359
                     /locus_tag="PK977_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF17982.1"
                     /translation="IAYYSLIDIRNHKLKLENNELHWHSVKTIKKLAFDHKMILDTCL
                     MRLQQQVMENPVVFNLLPEKFSLRELQELYEAILGYELDRRNFRKKISIKD"
     assembly_gap    3360..3562
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3719..>4228
                     /locus_tag="PK977_07435"
     CDS             3719..>4228
                     /locus_tag="PK977_07435"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF17983.1"
                     /translation="MFSSKFLKAAGLMVLALVAQISFAQNRVITGKVTDSKDGSAVQG
                     ANVIGKGTRTGTQTKSDGTFSISVENSVTVLVISSVGFTTQEVSVDGKTSVEVSLVQA
                     STSLGEVVVIGYGTARRKDLTGSIATVSTKDFAKGPLTSPEQLINGKVAGVQVTAPNG
                     APGAGGRIRV"
     assembly_gap    4229..4292
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4293..>4742
                     /locus_tag="PK977_07440"
     CDS             <4293..>4742
                     /locus_tag="PK977_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF17984.1"
                     /translation="LDQQSGIAGSNNPLSLINPNDIETFNILKDASATAIYGNRATNG
                     VIIITTKKGKTGKLRINFSTLNSISTVGDKVDVLTAEEFKQVVNGKGTNNQKNLLGAS
                     NTNWQDQVYSSAFMTDNNIALTGGIKGLPYRFSLGYLNQEGILKTGNL"
     assembly_gap    4743..4961
                     /estimated_length=219
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4962..>5228
                     /locus_tag="PK977_07445"
     CDS             <4962..>5228
                     /locus_tag="PK977_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17985.1"
                     /translation="DGLAPRNPVGRLYNRDDKSDVNRYITNLQLDYTFPFLKDLRANL
                     NIGYDYAKGEGTVVIPDSVGVDYIKGGTNNQYDNTKKNKLLDFYL"
     assembly_gap    5229..5404
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5405..6696
                     /locus_tag="PK977_07450"
     CDS             <5405..6696
                     /locus_tag="PK977_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF17986.1"
                     /translation="LISFYGRANFSINSKYLFTFSVRRDGSSRFSEDERWGLFPSAAF
                     AWNIAEESFLKNNKTLSALKLRLGYGETGQQEIGNDYAYIANYTLGTQTAQYQFGNTF
                     YQVNRPVGYDANIKWEQTSTFNIGLDFGFLNGKISGSIDYYNKETEDLLSFIDVPAGT
                     NFTNRIFTNVGDMNNKGVEFVLNSNILKKSKLTWDAGFNITYNKNEITKLTRVDDPTF
                     VGVPVGGISGAVGNTIQIHSVGYQPSSFFVLQQVYDNAGKPVEGLYVDRNGDGTINNL
                     DYYRYKTPEPLWLIGFTTSVAYDRWNFSTVLRGNIGNYMYNNMFSSNGVFQPGSLNFL
                     NNVHRNYLETGFGTYQYFSDYYVENASFLRMDNFTVSYDFGKVFNNKANLRATGSVQN
                     VFVVTKYRGLDPEVAGGIDNNFYPRPRVFSLGLNLEL"
     gene            6733..8328
                     /locus_tag="PK977_07455"
     CDS             6733..8328
                     /locus_tag="PK977_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF17987.1"
                     /translation="MRNSISKLLGLVAVAALFLASCTKDLDRQPVYGNTAASLYKDLA
                     GYKSVLAKTYAGLTMTGNSGPDGQGDVSGIDEGFSSYLRQYWSAQELSTDEAVIGWND
                     ATIKDFHFMTWSPADVFIRALYSRIFFQITLANEFIRESAADKVSGRGISGADATEIA
                     YMRAEARFLRALSYYHAMDLFGNVPFVTENDNVGAFLPNQISRANLFNYIESELKAIE
                     TELKDARTNEYGRADKAAAWALLSRMYLNAEVYLGAGNAKYTECITYCNKITAVSGYQ
                     LINNYRHLFVANNDVTGSSEFIFAVNFDGNRARTWGGTTFLVHAPIVGSMNAADYGVD
                     FGWGGIRTTKQFVQKFAGPAGDSRQNFYTSGQNLEINDISSANDGYGIVKFTNKTSTG
                     ANGSNLTWVDTDFAFFRLAEIYLNYAEAVLRGGTGGSSTTALNYINALRQRAYGNSSG
                     DITAPQLTVPFLLDERAKELHWEAVRRTDLIRYGQFTEGTYLWAWKGNTLNGQAVGNH
                     RKLYPIPSADLVANPNLAQNPGY"
     gene            8380..9471
                     /locus_tag="PK977_07460"
     CDS             8380..9471
                     /locus_tag="PK977_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HRF17988.1"
                     /translation="MKAIFKSSVLLLILSAALWSCEKDETRAVLNPGGATQLTVTPTT
                     LVLEQANAGNTAATFSWGKASFGYDAAINYVIQFCKGGTNFASTATTTEFNNEIAITK
                     SFTVKDLNAKLLDIIPYGSAQQVDVRIKASVGSNVDPIYSNMVTMTVTCYRDIINYSF
                     PQALWIAGNFQGWDPPSAPKIVDRFASGTTGSNYDGYINFTNASPEFKMVKGNNWGAG
                     DFGGSAGVLTNGGSNLTLSGGAGVYRITANTAGMTWSATKINSWAVTGNATPLGWPAG
                     PGGTPGQDHDMTFNAADGTWTITMNLNAGELKFRANDDWAINFGDNSPVDNKPDYGGS
                     NIQITTAGNYTITLDLGVGGNYAYTIRRN"
     assembly_gap    9601..9634
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9635..10582
                     /locus_tag="PK977_07465"
     CDS             <9635..10582
                     /locus_tag="PK977_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRF17989.1"
                     /translation="NNPAPLHPNFAKGADISWVTEMEGAGKKFYSASGVETDCIQLLK
                     NLGMNAVRLRVWVNPAGGWNNTADVVAKAVRAKNLGMRIMIDFHYSDSWADPGQQTKP
                     AAWASQDFTTLQQSLYNHTFDVLTALKTAGVTPEWVQVGNETNDGMLWPTGKASSSMA
                     NFAALVNRGYDAVKAVDPAIKVIVHISNGHDNNLFRWILDGLKNNGGKWDITGMSLYP
                     DPGNWAVMNTQCIGNINDLIYRYNKPVMICEAGMSWDQPDACKSFLSDLINKVNTASG
                     NNGLGVFYWEPQCYGNWKGYTKGAFDNSGKPTIALNAFN"
     gene            10617..>11183
                     /locus_tag="PK977_07470"
     CDS             10617..>11183
                     /locus_tag="PK977_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HRF17990.1"
                     /translation="MKYVGFLLVVFSLIAISTTAQRQQLNFDNDWKFHFGHAANPEKD
                     FNYSIVNIFSKSGAAVNTAIDPKFNDSKWRTLDIPHDWAVELPFVYKDNFDVMSHGYK
                     PVGALFPETSIGWYRKHFTVAKSDSGQRFQIQFDGIFRDANIWLNGFYLGNNKSGYVG
                     AAYDVTDYINFSGDNVLVVRVDGTQYEGW"
BASE COUNT         3123 a         2000 c         2330 g         2994 t
ORIGIN      
        1 gtgtatgttc atgtgggtgt cataacaaat ctgagtacca gttcttctga ctggcgatat
       61 gtacgattta cctggggaac aacaactgct gctgctcagg caacttcttt aggaagcaat
      121 cagtggcaat ataccatcaa taatccccgt acttactttt ctgtgccggc cggtgaaact
      181 attctaaaga ttgctattct tttcagaagt ggcaatggca atctaaaaca ggcaaataca
      241 gatggcagtg atatgtatat tccggtttat actactacta ttgctactaa gttctccgta
      301 ccaccttttc aacccacttt cattcccatt cccgagccta tcagtaaaca gcaaaacgat
      361 tttataaacc tgactgccat tgctaataat acagctgaga atatgaagtt gtatctaaat
      421 ggcacccaga ttgcatcggc aaacaatgta aatacaattt ctgcaaatcc tcagcttact
      481 gttgctggta accagaccat tgttgcagaa gctacgctga ccggaacgac ccgtactgat
      541 acacttcgtt tctttgtggc cagtgctcct gtggtagctc cgcttcctgc cggtgtaaga
      601 gatggaatca attatgagcc aggaaatact tccgtagtac tggtgttaaa tgcacccgga
      661 aagaacaggg tttctgtaat tggggatttt ccgggtagta attggattga acaaaccaat
      721 tatgtaatga acaaaacccc ggatggtaat cattggtggc tgcgaattac aggtttaaca
      781 cccggccagg aatattcatt ccaatacctg gtagacggaa cattaagagt aggtgacccc
      841 tatgctgaaa agattcttga tccatttaat gacggtttta taactgcggc tacttatccc
      901 ggtattaaac cgtatccaac aggcctgaca acaggaaatg taagcatact tcagacgaat
      961 gcaccagcgt ataactggac ggttactaat tttaaccgtc ctgataaaag aaaccttgta
     1021 atttatgaaa tgctgttaag ggactttgtt gcagcacatg actggaaaac gatccgtgat
     1081 acccnnnnnn nnnnagttat cttcagcgcc tgggagtgaa tgccattcaa ctaatgccat
     1141 tcaatgagtt tgatgggaat gaaagttggg gttataatcc tgcctacttc ctggcacccg
     1201 acaaatatta tggtcctaag aacagtttaa aggagtttat tgatacctgt caccgcagag
     1261 ggatagccgt tattatggat attgcattaa accatactac aggtgcttca ccgctggcag
     1321 ctttatattg gaattcctca actaatcaac ccgcagcaaa taatccctgg cttaatatta
     1381 cggctactca tccattcagt gtatttaatg atttcaacca cgaaagctta cgaacccggt
     1441 attttacaag ccgtgttatt gagcattggt tacaggagta taagctggat ggattccgtt
     1501 gggatctttc caaagggttt acacaaacca actcaggaag taatgtaggg ttatgggggc
     1561 agtacgatgc aagccgggtt gctatctgga agagatatta tgatacgatg caactgaaat
     1621 cgcctggcac atattgtatt ttagagcact ttgcagataa cagtgaggag atagaacttt
     1681 ccaattatgg aatgttactt tggggaaaca atactgctca atggcaggaa gcaacaatgg
     1741 gctacacaaa cacttcaaat tttgaaggaa atattcacag tgtccgtaat tggtccaatc
     1801 cccatttgat tggctatatg gaaagtcatg atgaagaaag attaatgtat aagaatttgc
     1861 agtttggaaa cactgcaggt acttacaata caaaagattt aaatactgca ctaaagcgta
     1921 tggaacaggc tgcagctttc ttcttcaata ttcccggacc caaaatgatg tggcaatttg
     1981 gagagttggg atacgattat tccataaatc actgccccga tggcaccatt aataataact
     2041 gtcgcacagc taataaaccc attcgttggg attataaaaa tgtgatacag cggcaacgtc
     2101 tttatgacat ctacagcgca atgaataagc tgcgctttca tccctggtat aaagatattt
     2161 atatcgccaa tgctgtttcg attgacagaa gccttgccgg cggatttaag tttttacggc
     2221 tcagaagtgc taatgataca tcctgtgtgg tagtagttgg aaattttgat gtagtgcagc
     2281 agagcggaac ggtaacattt ccggtagcag gcacctggta tgattatttg aatgggggaa
     2341 catttacagc aaccggcact gcccaaagtt ttaccttgca gccaggtgaa tacagggtgt
     2401 tgttaaaccg gaatctcacc aatgcagtag tgacttcaac tggtggatcg cctactgtat
     2461 cagctgcggg attaacggct tcattgttcc ctaaccctgc aaacagcaat tcggttttgg
     2521 agataaatgt accggaaaca ggtaaagtac aggttgaatt atataaccag ctgggtcaac
     2581 agattaaaac tgttttctct gacaggttat tgaaaggcaa tcatagaatt ccacttacag
     2641 gcaaagtgga caatttaccc gccggatcat atctgcttac tatacagcaa gccggtgtga
     2701 aagtatccgt aaaaatgatt attcagtaat tctaaaaatc ggcattagta tgtcaaaagc
     2761 actcaacctg aaaacagctg aaatagaaaa aatcagccac cttgattatt tcaatattgc
     2821 gatatctgtt gactgtgtca tttttggtta tgatgataaa gacctgaaag tattattgat
     2881 caaatcggat cttgaggagt tcgaaggtct ttattcatta ctgggcgacc tggtaaggcc
     2941 agatgaagac cttgaaacag cttcttaccg gatattgaaa gaaagaacag gccttgatga
     3001 tgtttacctt gagcaggtac aggcttttgg aagccttgac agnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nncattgctt attattcact aatagatatc cgcaaccata agttaaaact
     3121 ggagaacaat gaactgcatt ggcattctgt aaaaacgata aaaaaactgg ccttcgacca
     3181 taaaatgatt cttgacacct gtttgatgag gttacagcaa caggtgatgg aaaatccggt
     3241 agtctttaat ctgttaccag agaagttttc actcagggaa ttacaggaac tgtatgaagc
     3301 tattctcggt tatgaattag accggcgcaa ctttcgaaag aaaatatcta ttaaggactn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nngccccttt acaggggctt atttttatag cggagaagta
     3601 aaataaattt ttggctgatt taaataagtg ttaatattgt gtgtttaata cacatttagt
     3661 cgtgtagcaa atacatgata attgtgtaat ctgattgcta aaaaaataat gattgcttat
     3721 gttctcctca aaatttctta aggctgccgg ccttatggtg ctggctcttg tagcgcaaat
     3781 ttcctttgca cagaacaggg tgattaccgg taaggttaca gattccaaag acggatcagc
     3841 cgtacagggc gccaatgtta ttggtaaagg aaccagaaca ggtacacaga ctaaatcgga
     3901 tggtacattc agtatctctg tagaaaattc tgttaccgtg cttgtcatat cttctgttgg
     3961 atttaccact caggaagttt ctgttgatgg caagacctcg gttgaggtat cattggtgca
     4021 ggccagtact tcattgggtg aagtggtggt gattggttat ggtactgcca ggagaaaaga
     4081 cctgacaggt tccattgcta ctgttagtac taaagacttt gcaaaaggtc cacttacatc
     4141 acctgagcag ttgatcaatg gtaaagtggc tggtgttcag gttacagctc ccaatggtgc
     4201 tcccggtgcc ggggggcgta ttcgtgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctggatca gcaaagcgga atcgcaggta
     4321 gcaataaccc gctttcattg ataaacccca atgatattga aaccttcaat atcctgaaag
     4381 acgcctcagc aacagccatc tatggtaaca gggccacaaa tggtgttatc attataacaa
     4441 ctaaaaaagg aaaaacaggt aaactgagaa ttaatttcag caccctgaat tcaatttcta
     4501 ctgtagggga taaagtagac gtactgactg ctgaagaatt caagcaggta gtaaatggta
     4561 aaggaaccaa caatcaaaag aatttgctcg gggcctccaa taccaattgg caggaccagg
     4621 tgtattcttc tgcttttatg actgacaata atattgcact cacaggcggt attaaagggt
     4681 taccttatcg cttctcattg ggctatctga accaggaagg tatcttgaaa acaggtaatc
     4741 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatggtttg gcccctcgta
     4981 accctgtggg aagattgtat aacagggatg ataagagtga tgtgaacagg tatattacta
     5041 atcttcagtt agattataca tttcctttcc ttaaagatct gagagccaac ctgaatattg
     5101 gttatgatta tgcaaaaggt gaaggcacag ttgtaattcc tgactcagta ggtgttgatt
     5161 atattaaggg cggcaccaat aatcaatatg acaatactaa aaagaacaag cttcttgatt
     5221 tctacttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnncactta tatctttcta tggaagggca aatttctcaa taaacagtaa atatctcttt
     5461 acgtttagtg ttcgtcgtga tggatcatcc aggtttagcg aggatgaaag atggggctta
     5521 ttcccttcag cagcttttgc ctggaatatt gctgaagagt ctttcctgaa gaacaataaa
     5581 actctctcag ctttgaaact cagactgggt tatggtgaaa caggacagca ggaaataggt
     5641 aatgactatg cttacattgc caactacaca ttaggtacac aaacagcaca ataccagttt
     5701 ggcaatacct tttaccaggt aaaccgaccc gtaggttatg acgcaaacat taagtgggaa
     5761 caaacaagca ctttcaatat aggtttagac ttcggcttcc tgaatggtaa gatcagtggt
     5821 agtatcgatt actacaataa ggaaactgaa gatctgttga gctttattga cgttcctgcc
     5881 ggtaccaact ttaccaaccg tattttcacc aacgtcggtg acatgaataa caagggtgtt
     5941 gagtttgttt tgaattcaaa tattttgaag aaaagcaaac tgacttggga tgcaggcttt
     6001 aacattacct acaacaaaaa tgagattacc aagcttacaa gggttgatga tccaacattc
     6061 gtaggtgtgc cggttggtgg tatttctggc gctgtgggta atactataca gattcactca
     6121 gtaggctatc agccatcttc tttcttcgta ctgcagcagg tttatgataa tgctggcaag
     6181 cctgtagaag gcttgtatgt agaccgcaac ggcgatggta caatcaacaa ccttgactat
     6241 tatcgttata agactccgga gccactttgg ctgattggtt tcactacttc agtagcgtat
     6301 gatcgttgga atttctcaac agtattacgt ggaaatattg gcaattatat gtacaacaat
     6361 atgttttcca gcaatggagt attccaaccg ggttcactca atttcctgaa taatgtacac
     6421 agaaattacc tggaaaccgg ttttggaacc taccagtatt tctcagatta ttatgtagag
     6481 aatgcttctt tcctgcgtat ggacaacttt acagtttcct acgattttgg aaaagtattt
     6541 aataacaaag cgaaccttag ggctaccgga agcgttcaga atgtatttgt ggttacaaag
     6601 taccgcggac tggatcctga agtggcaggt ggaattgaca acaacttcta cccccgcccg
     6661 agagtattct cactgggtct taatctcgaa ttataataac aacgtaattg taaaaaataa
     6721 aaagcttgca aaatgagaaa cagtatttca aaattactcg gactggtagc agtagcagca
     6781 ctgttcctgg catcttgtac taaggacctg gacagacagc ctgtgtatgg taatacagct
     6841 gcctcattgt ataaagatct tgcagggtat aagtcagtat tggctaaaac gtatgcaggt
     6901 ctgacaatga ctggtaacag tggtcccgac ggacagggtg atgtatctgg tattgacgaa
     6961 ggcttttcca gttatctgcg ccagtattgg tccgcacaag agttgtcaac tgatgaagct
     7021 gtgattggct ggaacgatgc cacaattaag gacttccatt tcatgacatg gagtcctgcc
     7081 gatgtattca tccgggcatt atacagccgc atattcttcc agatcacttt ggcaaatgaa
     7141 ttcattcgtg aaagtgctgc tgataaagta agtggaagag gtatttcagg tgctgatgct
     7201 actgagatag cttatatgag ggcagaggct cgtttcctga gagcacttag ctattaccat
     7261 gcgatggatc tttttggtaa tgttccattt gttacagaga atgataacgt tggtgcattc
     7321 cttcctaacc agatcagccg ggccaatctt ttcaattata tagaaagtga actgaaagcc
     7381 atcgaaaccg aattgaaaga tgccaggaca aatgaatatg gccgtgcaga taaagctgct
     7441 gcatgggcat tactttcaag aatgtacctg aatgcagaag tatacctggg tgcaggtaat
     7501 gctaagtaca ctgaatgtat tacttattgc aacaaaatca ctgccgtttc cggatatcag
     7561 ctgatcaata attatcgtca cctgtttgtt gcaaacaatg atgtaaccgg ttccagtgaa
     7621 ttcatctttg cagtcaactt tgatggaaac agggcaagaa catggggtgg taccactttc
     7681 cttgtacatg caccgattgt aggatctatg aacgctgcag attatggcgt agactttggt
     7741 tggggtggta ttcgcactac caaacagttt gtacaaaagt ttgcaggacc agcgggtgat
     7801 tcaagacaga atttctacac cagtggtcag aatctggaaa tcaatgatat ttccagcgcc
     7861 aatgatggtt atggaattgt caagtttacc aataagacct caactggtgc caatggttct
     7921 aatcttacct gggttgatac tgattttgct ttcttccgtt tagctgagat ttacctgaac
     7981 tatgctgaag ctgtactgcg tggcggtact ggtggaagtt caacaacagc gctgaattat
     8041 atcaatgcac tgcgtcaaag agcatacggt aatagctctg gggatattac agctcctcag
     8101 ctaacagttc ctttcctgct tgatgaaaga gcaaaagaac tgcattggga agctgttcgt
     8161 cgtacagacc tgatccgtta tggtcaattc actgaaggta catacctctg ggcatggaaa
     8221 ggaaataccc tgaatggaca ggctgttggt aaccatcgca aactttatcc gattccttct
     8281 gctgacctgg tagctaatcc aaacctggca cagaacccgg gatattaatc ttttcaggtt
     8341 ttaaaacaga acacaactta tcattataaa aaaaatagta tgaaagcaat atttaaaagt
     8401 tcggttttgc ttttgattct ttcagcggcg ctttggtcat gtgaaaagga tgaaacaaga
     8461 gctgttctta atcccggcgg cgctacacaa ttaacagtta cgcctacaac cctggttctt
     8521 gaacaggcaa atgccggtaa tactgcggcc actttcagct ggggtaaggc cagcttcggt
     8581 tatgatgcag caattaacta tgttatacag ttttgcaagg gtggtaccaa ttttgcatct
     8641 actgcaacca ctacagagtt taataatgag attgcaatta caaaatcttt tactgtaaaa
     8701 gatctgaatg caaagttgtt agacatcatt ccttatggct ctgcacaaca ggtagatgta
     8761 aggataaagg cttctgttgg aagcaatgtt gaccccattt actcaaatat ggtaacaatg
     8821 actgtaacct gttacaggga catcatcaac tacagttttc cgcaggcgct gtggattgca
     8881 ggtaacttcc agggatggga tcctccgtca gctcctaaaa ttgttgaccg gtttgccagc
     8941 ggaactacag gcagtaatta tgatggctac atcaacttta ccaatgcctc gcctgaattc
     9001 aaaatggtga aaggcaataa ctggggcgca ggtgactttg gtggcagtgc cggtgtatta
     9061 acaaatggag gatctaacct tacattgtct ggaggtgctg gtgtatatcg tattacagct
     9121 aacactgccg gtatgacatg gtctgctaca aaaattaata gttgggctgt aaccggtaac
     9181 gctactccgt tgggttggcc cgctggcccc ggaggtacac ctggccagga tcatgatatg
     9241 acattcaatg cagctgatgg cacatggacc attacaatga atttaaatgc aggtgaattg
     9301 aaattcaggg caaacgatga ttgggcaata aacttcggtg ataattctcc ggttgacaac
     9361 aagccagatt atggtggtag caatatccag attacaactg caggtaatta tacaattacc
     9421 cttgaccttg gggtgggtgg aaactatgcc tatacaataa gaagaaacta aagcagggtt
     9481 atcagaataa agtaaagtgg ctgccttatg gcggccactt ttgtttttgt ggtaacttta
     9541 agtaataaaa tttctcaatg agaaagctat tgctgatttc atgctggctg ctgttttcct
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaataat ccagccccgc tgcatcccaa
     9661 ttttgctaaa ggtgcagaca ttagctgggt tacagaaatg gaaggagcag ggaagaaatt
     9721 ttattctgct tcaggtgttg aaactgattg catacaattg ctaaagaacc ttggtatgaa
     9781 tgctgttcga cttcgtgtat gggtgaaccc tgcaggtgga tggaacaata cggctgatgt
     9841 agtggcaaaa gcagtaaggg caaagaatct gggcatgcgt atcatgattg attttcatta
     9901 tagtgattca tgggctgatc cgggacaaca aactaaaccg gcagcatggg cttcacagga
     9961 ttttaccaca ctgcaacaat ctttatataa ccacactttt gatgtattga ctgcattaaa
    10021 gactgccggt gtaacacctg aatgggtcca ggttggcaat gaaacgaatg acggaatgtt
    10081 atggcctacc ggtaaggcat cttccagtat ggccaacttt gctgcactgg taaacagagg
    10141 atatgatgct gtaaaggctg ttgatccggc cattaaagtg atcgttcaca tttcaaatgg
    10201 tcatgacaat aatttattcc gctggatact ggacggctta aagaataatg gaggaaaatg
    10261 ggacattacg ggaatgtcat tgtacccgga tcctggaaac tgggccgtta tgaatacaca
    10321 atgcattggc aatatcaatg atttgattta ccggtataat aagccagtaa tgatatgcga
    10381 agcaggaatg agctgggatc agccggatgc ctgtaagtct tttctttctg atcttatcaa
    10441 taaagttaat acagcatcag gtaataatgg gttgggcgta ttttactggg aacctcagtg
    10501 ctatggaaac tggaaaggat atacaaaagg cgcttttgat aattctggca agccaacgat
    10561 tgcgttaaat gctttcaact gatatttcgc ctcacatact ttggtaactt tccataatga
    10621 aatatgtagg ctttttatta gtggtattta gtttaattgc tattagtact actgcacaaa
    10681 gacagcagct caattttgat aatgactgga aatttcattt tggtcatgct gctaatcctg
    10741 aaaaagattt caattacagc atagttaata ttttttccaa gtctggtgct gctgtaaata
    10801 ccgccataga tccaaagttc aacgacagta aatggaggac attagatatt ccgcatgact
    10861 gggctgtgga attacctttt gtatacaaag acaattttga tgtgatgtct catggttata
    10921 aacccgtcgg agctttgttt cccgaaacaa gcataggatg gtaccggaaa cattttacag
    10981 tggctaaatc cgattcaggt cagcggtttc aaatacaatt tgatggtatt tttcgtgatg
    11041 ccaatatctg gctgaacggt ttctatttgg gcaacaataa gagcggttat gtgggagctg
    11101 catatgatgt tacggattat atcaatttca gcggggataa tgtgctggtg gtaagagtgg
    11161 atggcacgca atatgaagga tgg
//
LOCUS       DAORLS010000075        11106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3151_length_11106_cov_1.974572, whole genome shotgun sequence.
ACCESSION   DAORLS010000075 DAORLS010000000
VERSION     DAORLS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_11106_cov_1.974572"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PK977_07475"
     CDS             <1..265
                     /locus_tag="PK977_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF17991.1"
                     /translation="SQYAVATVEYRYLVGQNSFFFVLADGGWGRDASRGQQLNYTYFG
                     TGLGLAFETKAGIFNLAWAIGKRNDTEFNLRQSKIHFGFVNYF"
     gene            320..1297
                     /locus_tag="PK977_07480"
     CDS             320..1297
                     /locus_tag="PK977_07480"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRF17992.1"
                     /translation="MNRLIILFCCFLSFTAVAQLPADTAFLTPPPDSLLQTDSTATTD
                     TIALSVTKADSVSSKPKNDSAWVISSDIINNPAKLTKLILDQHPYFDFKTKAASPPVS
                     EKRVVEGKELLFYLLVFLLIVFAVLRQLFPKYFSDLFRLFFRTTLKQRQIREQLMQTP
                     LPSLMLNGFFVISAGLYISFLLQHFAVSPVEDFWLIFFYASIGLSVAYFVKFIGLKVS
                     GWLFNRSEAADSYIFIVFIINKMIGILLLPFLVILAFAVGNVYTVGLTLSWCLLGGLL
                     VYRLLLSFGAVRNQVRVNLFHFFLYLCAFEIAPLLLVYKGLLLYFSITT"
     gene            1327..>1828
                     /locus_tag="PK977_07485"
     CDS             1327..>1828
                     /locus_tag="PK977_07485"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRF17993.1"
                     /translation="MTKTGVNKADVQAKAIKKILITQPRPDSEKSPYFELGRKYNVEL
                     DFYPFIKLEGIPARDFRKQKIEIQNYTGIIFTSRNAIDHFFRMCEEMKVNISQDTKYF
                     CITEAVALYLQKFILYRKRKVFYGADGTNKSMFDVINKHKANEKFLYVCSENQQDNEI
                     VNWLKAN"
     assembly_gap    1829..1841
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1842..2135
                     /locus_tag="PK977_07490"
     CDS             <1842..2135
                     /locus_tag="PK977_07490"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRF17994.1"
                     /translation="EFTLAFLYRSVSSDVKEILQKNEYDVICFFTPSGVKSLFDNIPK
                     YKQNGTVIGAFGNNTSKAIEDAGLKLEIKAPQPQTPSMVAALEQFLAATAKKK"
     gene            2231..>3432
                     /locus_tag="PK977_07495"
     CDS             2231..>3432
                     /locus_tag="PK977_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRF17995.1"
                     /translation="MAKHTNQLVNESSPYLLQHAHNPVNWYPWGDEALQKAKEENKPI
                     LVSIGYAACHWCHVMERESFEDESTAAIMNEHFINIKIDREERPDLDHIYMDAVQAMT
                     GSGGWPLNVFLTPDAKPFYGGTYFPLQRAFNRPSWQETLYRVIQAFRERRHEIDAQAE
                     NLTEHLTQSNAFGLKKINEDELFSLENIHIVFQNIMKSADRLWGGFGKAPKFPQTFSI
                     QFLLRYHYHTGNKEALTQALLSLDKMIDGGIYDQLGGGFARYSTDTEWLAPHFEKMLY
                     DNALLVSVLCEAFQVTGKKRYREVIDETLEFVQRELLHSSGGFYSALDADSEGVEGKF
                     YTWSKDEIEQLLGNDTTLFCDYYDVSAGGNWEHTNILWVRKSMINFAANNGIPEEELN
                     KVLHRCRE"
     assembly_gap    3433..3442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3443..>3951
                     /locus_tag="PK977_07500"
     CDS             <3443..>3951
                     /locus_tag="PK977_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRF17996.1"
                     /translation="MQARSKRIRPLLDDKIILGWNALMNTACSKAYAATGAENYRKLA
                     IENMNFLLQYFAVNGTEEFHHTWKNNTARYPAFLDDYAFLIEALMHLQEVTGDTEWIK
                     KAKLITEHVMGHFSEAETGFFYYTKTGQRDVIVRKKEVYDGAVPSGNSVMAKNLWQLS
                     ILFDKPEWR"
     assembly_gap    3952..3963
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3964..4310
                     /locus_tag="PK977_07505"
     CDS             <3964..4310
                     /locus_tag="PK977_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRF17997.1"
                     /translation="RSIDMTSSLGNAIVRYPTSFGNWAGLLMEFVTGTNEIAIVGEEA
                     GQLCSQVLQAYIPQRVIMSSSLVDKDYPLLAGKPAGDKTSIYLCRSYTCQRPVFSVNE
                     LMLLIDKNKNRQ"
     gene            4462..5775
                     /locus_tag="PK977_07510"
     CDS             4462..5775
                     /locus_tag="PK977_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF17998.1"
                     /translation="MKNLYYALYAAACMTMLASCGILGKKNGKGKGGSLPNDGQVHGV
                     APGNKYSLPKPPGMVYVPQGTFHMGPSDEDPAYAFSARNRSVSISGFWMDATEITNNE
                     YRQFVQWVRDSVVARKMGYVKAGADGNEYVDWKKMQTMKWSDPKVMEQLSSTDIILPP
                     DDRIFGKKEIDPSKIIYHSEVFDLKEAAKRENAGKPRSKFIVKKDTPIYPDTLCWIRD
                     FSYSYNEPMTKRYFSHPAFGNYPVVGVSWKQATAFCEWRTHLLNSYLDSKKRTQESDF
                     RLPTEAQWEYAARGGRSQSMFPWGNYYLRNKKGCLMANFKPGRGNYPEDGGFYTVRAD
                     AYWPNDYGLYCMSGNVAEWTSSIYYEGRNNFQHDMNPDVRWNAKDDDPPLMKRKIIRG
                     GSWKDVGYYLQTGTSTYEYQDTTKSYIGFRCVIDLPAAPAKGRKK"
     assembly_gap    5779..5829
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5890..6663
                     /gene="gldL"
                     /locus_tag="PK977_07515"
     CDS             5890..6663
                     /gene="gldL"
                     /locus_tag="PK977_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRF17999.1"
                     /translation="MAAAIPSKVSKWVDVGVSFGASIVILGALFKIVHHPLADTFLWF
                     GLVTEAVIFAIYGILYAIYPPIKDATHDEEQFSVAGAKSRAAFGAMDKMLAEADITPD
                     TMRRLGDGFKQLNTTVSGMNNIGDTIAATTEFTAKTREVTGQLDKVKDAYTTAASSVG
                     AFNTATEGAKVFHEQIQVLTKNLSSLNTIYELELQESNNHLKALNAFYGKLSETSSAM
                     LNSAEDAKRVQEQIGNLATNLGRLNNIYGNMLTAMQGRN"
     gene            6729..>7292
                     /locus_tag="PK977_07520"
     CDS             6729..>7292
                     /locus_tag="PK977_07520"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HRF18000.1"
                     /translation="MALPKEPRQKMINIMYLVLTALLALNVSSEILNAFKTVNRSLES
                     TNTTVNKSTETIINSLKEKATKAETAERANYWLPKAEKVVEFSKKVYDFIATLKGKIL
                     TEAGGSTTDPNKSFKEDNLDVVTRIMIKEGKGNELKKLLEDYTKNIKGIDPKIDSAFT
                     NAFVDLSNPPGRDGKTKAWDIAYFNMVP"
     assembly_gap    7293..7526
                     /estimated_length=234
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7527..8276
                     /locus_tag="PK977_07525"
     CDS             <7527..8276
                     /locus_tag="PK977_07525"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRF18001.1"
                     /translation="SIGGTVVPIGEEGFALYKTQAGGVGSRSVPVKITYFNQTTGKEE
                     VKEVNVEYTVGSANASIALDKMNVLYIGVDNPVSISASGGGDDKVQATISGGGGSLNR
                     VGNGKYVARVTQQSDDCKITVTVDGKVMGVAQFRVRQIPGAVATVGGFQSGENIPAGT
                     FRAQGGVAAWIKDFPFDLKYTVTQFTISTDSDDGDIVEAVCTGNAFSPKAQQIIRDQV
                     KAGKTVYIDNIRVLGEDGQNKKVPSLVYYIK"
     gene            8289..9314
                     /gene="gldN"
                     /locus_tag="PK977_07530"
     CDS             8289..9314
                     /gene="gldN"
                     /locus_tag="PK977_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRF18002.1"
                     /translation="MKNNLIKATVCLVLVAGLYADADAQRQPRRRTNNPANNQGNNPQ
                     GNEQQNNNNTAPPTYNPAVSVPITYDTSGAATGAPKPSLRNDNAFDKGSLTARTPLPY
                     EHLRWDDALFAEKVWRELDLREKMNQTFRYQAQDDNGSQIFLDILLKAVKKGDVTAWT
                     DDRFTTPLSMDELNQKVAGGYDTADRVDINDPSKIVARVVTLKTFDPNSVVKLRIKEE
                     WVFDRESSRMFCRILGIAPVQTVFAENGKERGQTVMFWIYYPDLRATLAKYEVYNPKN
                     MGQSRMTWEELFESRMFSSYILKSTMDNAANKNIRQYIKDPILALLEGDNIKEKIFNF
                     EQDLWSY"
     assembly_gap    9383..9438
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9565..>10462)
                     /locus_tag="PK977_07535"
     CDS             complement(9565..>10462)
                     /locus_tag="PK977_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HRF18003.1"
                     /translation="RVRTTFNSDATEIVVPFPIDYPQQNTTVTVPKGGDRKKLLELSE
                     KNVNHFIEEIKNKERLQLNVKGKEKMPLLEQLQANLQLQDLPVHIECFDNSNFQGSYP
                     VSAMVCFKYGEPSKKDYRHFNVKTVEGINDFATMKEAVYRRYKRLKEENQPFPQLVII
                     DGGKGQLGAALEAANELGLTSSITFLGLAKNVEEIFFAGDKESLKLPYNNESLKFIRR
                     IRDEVHRFGISFHRQKRSKGTFKNELEEIKGIGKATTDLLLKEFKSVKKIKEASTEKL
                     EKLVGKVKADVISRHFTKQRDT"
     assembly_gap    10463..10496
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10497..>11106)
                     /locus_tag="PK977_07540"
     CDS             complement(<10497..>11106)
                     /locus_tag="PK977_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRF18004.1"
                     /translation="TYPFLVIKKEPFPRVFLTRKKLDDGSEYLGPYTSVKKVRELLEF
                     IKQTIQLRTCSLNLSENNIRKGKFKVCLEYHLGNCKGPCEGFQTPANYQEGLAQLKNL
                     LRGNLSPVIKHYKNEMKTLAEGLEFEKAALVKKKIEFLENYQSRSVVANTRTGNVDVF
                     TIQKVSLPDRQQKDIACFNYLMVRNGSIIQTHTGKVETHLDET"
BASE COUNT         3045 a         2134 c         2403 g         3114 t
ORIGIN      
        1 gtcgcagtat gccgtggcca ccgtggaata ccggtatttg gtggggcaaa attccttctt
       61 ctttgtgctg gcagatggcg gctggggccg ggatgccagc agggggcagc agttgaatta
      121 tacttatttc ggtaccggcc tgggtttggc ttttgaaact aaggctggca tttttaacct
      181 ggcatgggcg attggtaaac gaaatgatac agaattcaac ctgcgccaat cgaaaattca
      241 ttttgggttt gtgaactatt tctaaaagaa caactgatta gttgcttact tcaatatttt
      301 tgcgccacaa cttcactgat tgaaccggct catcattttg ttttgttgtt ttttatcttt
      361 tacagcagtt gctcaattgc ctgcggatac cgctttttta acgccacctc cggattcatt
      421 actgcaaact gacagcaccg ccaccacgga tactattgct ttatctgtta ccaaggcgga
      481 ttctgtttcc tcaaagccca aaaacgattc tgcatgggtt attagttctg acattatcaa
      541 taacccggca aagcttacta aactgatact tgatcaacat ccttattttg attttaaaac
      601 aaaagcggca tcgccccccg tgtcagaaaa aagggtagtg gaaggcaagg aactcttgtt
      661 ttacctgtta gtgtttctgc taatagtatt tgccgtacta cgacagttat ttcccaaata
      721 cttcagtgac ttgttccggc tcttcttccg gactaccctg aagcaacgcc agatcaggga
      781 gcagttgatg caaaccccat tgccctcctt aatgctgaat ggtttctttg taatcagcgc
      841 cggtttgtac attagttttt tattacagca ttttgctgtt agcccagtag aagatttctg
      901 gctcatcttt ttttatgcat ccatcggtct gtcggttgct tattttgtca agtttatcgg
      961 gctaaaggtt tcgggctggt tgtttaatag gagtgaggca gctgattctt atattttcat
     1021 tgtttttatt atcaacaaga tgatcggaat actgctgctg ccttttttag ttatcctggc
     1081 attcgctgtg gggaatgtgt atacggtggg gctcacactc tcgtggtgcc tcctgggcgg
     1141 actgttggtt tacaggctcc tcttatcgtt tggagcagtc cgtaatcagg tcagggtcaa
     1201 cctttttcat ttttttcttt atctctgtgc ttttgagatt gcccctcttt tactggttta
     1261 caagggcttg ctattgtatt tcagtataac tacttaactt tgcagccaac tttagccgaa
     1321 ccgaaaatga ctaaaacagg ggttaataag gccgatgtgc aggccaaagc gatcaaaaaa
     1381 atcctgatta cccaaccgag acccgacagt gaaaagtcgc cgtactttga attgggccgg
     1441 aagtataatg tggagctgga tttttatcct tttattaaac tggaaggtat acctgcccgt
     1501 gatttccgca agcaaaaaat cgaaatacag aactataccg gtatcatttt caccagccgc
     1561 aatgctattg atcatttctt ccggatgtgt gaggaaatga aggtcaatat atcgcaggac
     1621 accaagtact tctgtatcac tgaggccgta gccctttacc tgcaaaagtt cattctttac
     1681 cgcaagagaa aagtatttta cggtgcagat ggcaccaata aaagtatgtt tgatgtcatc
     1741 aacaagcaca aggctaatga gaagttcctg tatgtatgtt cagaaaatca gcaggataat
     1801 gagattgtca actggctaaa ggcaaatann nnnnnnnnnn ngaatttacc ctcgctttcc
     1861 tgtaccgcag tgtgagcagc gatgtaaagg agatattgca gaagaatgaa tatgatgtta
     1921 tctgtttctt tactcccagt ggtgtaaaaa gtttatttga taatattcca aaatacaaac
     1981 aaaacggtac tgttattggc gcttttggca ataatacatc caaagccatt gaagatgccg
     2041 ggctgaagct ggaaataaaa gctccacaac cacaaacccc cagcatggta gcggctttgg
     2101 agcaattcct ggcggcaact gccaaaaaga aataattcta caaaagagct gatacaaaaa
     2161 agagtacttt gacagtactc ttttttacat tttgccacta actagtgcga catttacttt
     2221 cttttttatt atggcaaaac atacaaatca gctggtcaat gagtcaagcc catacctgtt
     2281 gcaacatgcc cacaacccgg tgaactggta cccttgggga gatgaggctt tgcaaaaagc
     2341 caaagaagag aataagccga tactggtaag cattggttat gcagcttgcc actggtgcca
     2401 tgtgatggaa cgggagagtt ttgaagatga gtccactgct gccatcatga acgaacattt
     2461 tatcaatatt aaaatagaca gggaagaacg tccggacctg gatcatattt atatggatgc
     2521 ggtgcaggcg atgacaggaa gcgggggctg gcctttaaat gtttttttga ctcccgatgc
     2581 caaacctttt tacggaggaa cctattttcc cctccaaagg gcttttaacc gtccctcctg
     2641 gcaggaaact ttgtacaggg taatacaggc attccgtgag aggagacatg aaatagatgc
     2701 acaggctgaa aaccttaccg agcacttaac ccaatccaac gcttttggtt taaagaaaat
     2761 aaatgaggat gaattattct cattggaaaa tatccatatc gtctttcaaa acatcatgaa
     2821 aagtgctgac aggctatggg gaggttttgg taaggcgccc aagtttccac aaacattttc
     2881 tatacagttc ctgctgcgct atcattatca tacaggtaat aaagaagccc tgacccaggc
     2941 gttactcagt cttgataaaa tgattgatgg aggtatttat gatcagttgg gaggaggctt
     3001 tgcccgttac tccaccgata cagagtggct ggcccctcat tttgaaaaaa tgctgtatga
     3061 taatgccctg ctggtatcgg tattatgtga ggcttttcaa gtaactggta aaaagcgtta
     3121 cagggaagtg attgatgaga ctttggaatt tgtacagcgg gaattgcttc attcatcagg
     3181 tgggttctat tctgcacttg atgcagatag cgaaggagtg gaaggtaaat tctacacctg
     3241 gtcaaaagat gaaatagaac aattgttggg aaatgataca acattgttct gtgattatta
     3301 tgatgtgtca gcagggggta actgggagca taccaatatt ctgtgggtac gaaaatcaat
     3361 gataaatttt gcagccaata atggaattcc tgaagaagaa ctgaataagg ttttgcatcg
     3421 ttgcagagag aannnnnnnn nngatgcagg caaggagcaa gcgaatccgg cctttgctgg
     3481 atgacaagat catccttggt tggaatgctt taatgaatac tgcctgcagc aaagcctatg
     3541 ctgctaccgg cgcagagaac taccggaaac tggcgattga aaacatgaac tttttgcttc
     3601 aatattttgc tgtgaatggt actgaagaat ttcatcatac ctggaaaaac aacacagcga
     3661 ggtacccggc atttctggat gattatgcgt ttttgattga ggcattaatg catttacagg
     3721 aggtgacagg agatacagaa tggataaaga aagctaaatt aataacagag catgtaatgg
     3781 gtcatttcag tgaagctgaa acagggtttt tctattacac aaaaacagga caaagggatg
     3841 taattgttcg aaaaaaggaa gtatacgatg gagccgttcc ttccggcaat tcggtgatgg
     3901 ctaaaaatct ctggcagctt tccatccttt ttgataagcc tgaatggcgg cnnnnnnnnn
     3961 nnnaacgaag catagatatg acatcttctt tgggaaatgc gatcgttcgt tatcctactt
     4021 cttttggtaa ttgggcaggg ctattaatgg aatttgtgac cggaactaat gaaattgcta
     4081 ttgttgggga agaggccggg caattgtgca gccaggtatt gcaagcttat attcctcagc
     4141 gggttatcat gtcatcttct ttagttgata aggactatcc attgttagcc ggaaaacctg
     4201 ccggtgataa aacttctatt tatctctgtc gtagttacac ctgtcagcgt cccgtatttt
     4261 ccgtaaatga gcttatgttg ctgatagaca agaacaaaaa ccggcaataa tttttttgtt
     4321 aatacaataa atcaaggtca gataccgtta gaaaatttac cccgtaaaag acgctttgta
     4381 ttgaaaaaaa taaattttaa cgttgtgtca gaaaacaggt caaaacatta tatttgccca
     4441 atacccctta agtgtatgaa aatgaaaaac ctttactacg ccctgtacgc agccgcatgc
     4501 atgactatgc tggccagttg cggtatcctg ggaaagaaga atggaaaagg aaaaggcgga
     4561 agcctgccga atgatggcca ggtgcatggt gttgccccgg gtaacaaata cagccttccc
     4621 aaacccccgg gaatggtgta tgtgccccag ggtacgttcc atatgggtcc cagtgatgag
     4681 gatcctgcat atgcctttag tgcccgtaac cgttctgtat ctatcagcgg cttctggatg
     4741 gatgccactg aaattaccaa caatgaatat cgccagtttg tccagtgggt tcgtgactca
     4801 gtggtagccc gtaaaatggg ttatgtgaaa gccggtgctg atggtaatga gtatgtagac
     4861 tggaagaaaa tgcaaaccat gaagtggagc gatccgaagg tgatggagca gctcagcagt
     4921 actgatatta tccttccccc ggatgacagg atttttggta aaaaagaaat tgacccttca
     4981 aaaattattt atcactctga agtgtttgat ctgaaagagg ctgccaaaag agaaaacgcc
     5041 ggcaagcccc gttcaaagtt cattgtaaag aaagatactc ctatctatcc cgacaccctt
     5101 tgctggattc gtgatttctc ttattcttat aacgagccga tgaccaagcg ttatttttcg
     5161 catccggcct ttggcaatta tcctgtggta ggtgtaagct ggaagcaggc taccgctttc
     5221 tgtgagtgga gaacacattt gcttaattca taccttgact ctaagaaaag aacccaggaa
     5281 tcagatttcc gcctgccaac tgaggcacaa tgggagtatg cagcccgtgg cggacgttcg
     5341 cagtccatgt tcccgtgggg taactattac ctgcgtaata agaagggatg tttgatggct
     5401 aactttaagc ccggccgtgg taactaccct gaagatggcg gattctatac tgttcgtgcc
     5461 gatgcatatt ggcccaatga ttatggcctg tattgtatgt caggtaatgt agcagagtgg
     5521 acttcttcta tttattacga aggtcgtaat aacttccagc atgatatgaa ccccgatgtt
     5581 cgctggaatg ccaaagatga tgatcctccc ctgatgaagc gtaagatcat ccgcggcggc
     5641 agctggaaag atgtgggcta ttacctgcaa acaggtacca gcacatacga gtaccaggat
     5701 acaaccaaat cttatatcgg ctttcgttgt gttattgact taccggcagc tcctgcaaaa
     5761 ggccgcaaaa aataatttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnng tcctctcttc tttgtcactt caaactttca acttaaaacc actttaaaaa
     5881 atccaattta tggcagcagc aattccatct aaggttagca aatgggtaga cgtaggcgta
     5941 tcattcggtg cctccatcgt aattctaggt gccctgttta aaattgtaca ccacccgttg
     6001 gctgatacgt ttttgtggtt cggtctggta accgaagccg tcatctttgc tatttacggt
     6061 atcttatacg ctatatatcc tcctattaag gatgcgacac atgacgaaga gcaattttct
     6121 gttgccggtg ccaaatccag ggctgccttt ggtgccatgg ataaaatgct ggctgaagca
     6181 gacatcactc ctgacactat gagacgcctg ggtgatggtt tcaagcaatt gaacaccacc
     6241 gtttcaggta tgaataatat tggtgatacg attgctgcta ctacagaatt cactgctaaa
     6301 acaagagaag taactggtca gcttgataaa gtaaaagatg cctatacaac tgctgcttca
     6361 tccgtggggg catttaatac cgctactgaa ggtgcaaagg tgtttcatga gcagatacaa
     6421 gtgcttacta agaacctttc ttctttgaat accatttatg aactggagtt gcaggaaagt
     6481 aacaaccacc tgaaagccct gaatgcattc tatggtaaac tttctgaaac atcttctgcc
     6541 atgcttaaca gcgctgagga tgccaagaga gtgcaggagc agatcggtaa ccttgctact
     6601 aacctgggcc gcctgaataa tatctatggt aacatgctta ctgctatgca gggtcgcaac
     6661 taaggagctg taactaaaga agatttaaaa gagtcatcat tcataatcca atttaaacct
     6721 aaatagtcat ggctttacct aaagagcccc ggcagaagat gatcaatatt atgtacctgg
     6781 tgctgacagc cctgttggca ctcaacgtat catctgagat cctcaatgcc tttaaaactg
     6841 ttaaccgcag tttggaaagc accaatacaa ctgtaaataa atcaacagag acaatcatta
     6901 actctttgaa agaaaaagca acaaaggctg agacagcaga acgggcaaat tattggttgc
     6961 ccaaggctga aaaagttgtt gagttttcta aaaaggtata tgactttata gctacattaa
     7021 aaggaaagat cctgaccgaa gcaggaggaa gtactacaga tccaaacaaa tcattcaaag
     7081 aagataattt ggatgttgta acccggataa tgattaaaga aggcaaggga aatgagctaa
     7141 aaaagttact ggaagattat acaaagaata tcaaaggcat agatcccaag attgacagtg
     7201 ctttcacaaa tgcattcgtt gatttatcca atcctccggg ccgtgatggc aagacaaaag
     7261 catgggacat tgcttatttc aatatggtac cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnagta ttggaggtac ggtagtacct attggcgaag
     7561 aaggatttgc cttgtataaa actcaggcag gtggtgtggg tagccggagc gtaccggtta
     7621 agattactta ttttaaccag acaaccggta aagaagaagt aaaagaagta aatgttgagt
     7681 acacggtagg ttcagccaat gcatccatag cattggacaa aatgaatgtt ctttatatag
     7741 gtgttgataa tcctgtttct atttctgcca gtggtggtgg cgatgacaaa gttcaggcga
     7801 ccatttccgg tggtggcgga tccttaaaca gggttggtaa cggaaaatat gtggcaaggg
     7861 taacccagca aagtgatgat tgcaaaatca cagtaacggt tgatggtaaa gtaatgggtg
     7921 tagcgcagtt ccgtgttcgt cagatacccg gtgccgtggc aacagtaggt ggttttcaaa
     7981 gtggtgaaaa tatacctgcc ggaaccttca gggcacaggg tggtgtggct gcatggatta
     8041 aagactttcc gtttgacctg aaatacacgg tgacccagtt tacgatcagt accgacagtg
     8101 atgacggaga tattgtggaa gctgtatgta ctggtaacgc attcagccct aaggcccagc
     8161 agattatcag ggatcaggtt aaagcgggta aaactgtcta tattgataat attcgtgtgt
     8221 tgggtgaaga cggtcaaaat aagaaagtgc cgtcgttggt ttattacatc aagtaaatga
     8281 gataatacat gaaaaacaat cttattaaag caactgtgtg cctggttttg gtagccggat
     8341 tgtatgctga tgctgatgcg cagcgtcagc cgagaaggag aacgaataat cctgccaata
     8401 accaggggaa taacccgcag ggtaatgagc agcagaataa taataatact gctcctccaa
     8461 catataatcc tgctgtaagt gttcccatca cctacgatac ttccggtgct gctacaggtg
     8521 ctcccaagcc ttccttacga aatgataatg catttgataa ggggagtttg acagcccgca
     8581 cacccttgcc ctatgagcat cttcgctggg atgatgccct ttttgcagaa aaagtttggc
     8641 gagagctgga tttgagagaa aaaatgaacc agactttccg ttaccaggct caggatgata
     8701 atggtagcca gattttcctg gatattctgc tgaaagcagt taaaaagggt gatgtaactg
     8761 cctggaccga tgacaggttc actactccgc tttcaatgga tgaattgaat caaaaagtgg
     8821 ccggtggtta tgatactgct gacagggtgg atatcaacga ccccagtaag attgttgcaa
     8881 gggttgttac tttaaagact ttcgatccca atagtgttgt caaactaagg ataaaggaag
     8941 agtgggtatt tgaccgtgaa tcaagccgca tgttttgccg tattttaggg atagcccctg
     9001 ttcagactgt ttttgctgaa aatggtaagg aaagaggtca gacagttatg ttctggatct
     9061 actatcccga tctgagggca actttggcta aatacgaggt ttacaatcct aaaaacatgg
     9121 gtcaaagccg gatgacctgg gaagagcttt ttgaaagccg tatgttcagt agttatatac
     9181 tgaaatcaac gatggacaac gctgccaata aaaatatcag gcagtatatt aaagacccta
     9241 tcctggcact gctagaaggt gataatatca aagaaaagat cttcaatttc gagcaggatc
     9301 tctggtctta ttaatagcta aaaattaatg tgattttaat atgaaaggct cctttaaagg
     9361 agcctttcat attaaaatca cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnaa ttgttgtatc ttcgtttcgg tttttcatag gatattggat
     9481 tttaaaaacg gggctgaatt tctattctgg ccccattttt tttgcccttc cctgcctaca
     9541 aggtgtcata tcttctccat cctattaggt gtctctttgt ttggtaaaat gcctggaaat
     9601 aacatctgcc ttcaccttac ccactaattt ttctaatttc tctgtgctgg cttctttaat
     9661 ttttttcact gatttgaact ctttcagcaa taaatcggtc gtggctttgc caataccctt
     9721 tatttcttcc agttcatttt taaaagtgcc tttgcttctt ttctgacggt gaaaagaaat
     9781 accgaaacgg tgtacttcat cacgaatgcg acggataaat ttgaggcttt cgttgttata
     9841 cggcagtttc agggattcct tatcgcccgc aaaaaagatc tcctctacat tctttgccag
     9901 gcccaagaaa gtgatagaac tggtcagccc taattcattg gctgcttcca gtgcagcacc
     9961 caactgaccc ttacccccat caatgataac caactgcggg aaaggctggt tttcttcttt
    10021 tagtcttttg taccttctgt atactgcttc cttcatggtg gcaaaatcat taatgccttc
    10081 cactgttttt acattgaagt ggcgatagtc cttcttgctt ggttcaccgt atttgaaaca
    10141 aaccatcgca gataccggat aactgccctg aaagtttgag ttatcaaagc attcaatatg
    10201 tactggtaaa tcctgtaatt gaagattggc ctgcaattgt tccaacaggg gcattttttc
    10261 ttttcctttt acatttagct gaagcctttc tttattcttt atttcctcaa taaaatgatt
    10321 tacatttttt tcggaaagct caagtaattt ttttctgtca ccacctttag gtacggttac
    10381 agtggtgttt tgctgaggat aatcaatagg aaaaggtacg actatctccg ttgcatcact
    10441 attgaaagtg gttctgaccc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgt
    10501 ttcatccaaa tgcgtttcaa ctttgcctgt atgtgtctgg atgatgctgc cgtttcgaac
    10561 catcaggtaa ttaaagcagg cgatatcttt ttgctgcctg tcgggcaggc tgactttttg
    10621 aatggtaaag acatcaacat tgccggtccg ggtgttagcc actactgaac gggattggta
    10681 gttttccaga aattcaattt tcttttttac taaagctgct ttctcaaatt caagaccttc
    10741 ggccaatgtc ttcatttcat ttttatagtg ctttattaca ggggaaagat tgccccgtaa
    10801 caggttcttt aactgggcaa ggccttcctg gtaattggcc ggcgtttgaa aaccttcaca
    10861 ggggcctttg caatttccaa gatgatactc gaggcaaact ttgaatttac cttttctgat
    10921 attattctca gacaggttta gggagcaggt tcttagttga atggtttgtt ttataaactc
    10981 cagcagttct ctaacttttt ttacagaggt gtatggcccc aggtattctg atccatcatc
    11041 caattttttc ctggtaagaa atacccgggg aaagggttct ttttttatta caaggaaggg
    11101 ataggt
//
LOCUS       DAORLS010000076        11092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3156_length_11092_cov_2.159826, whole genome shotgun sequence.
ACCESSION   DAORLS010000076 DAORLS010000000
VERSION     DAORLS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3156_length_11092_cov_2.159826"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>264
                     /locus_tag="PK977_07545"
     CDS             <1..>264
                     /locus_tag="PK977_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF18005.1"
                     /translation="GVAKYGAEPALGYTASMFHLFTHAMFKALLFLGAGAVIHYVHSN
                     EMNDMGGLRKYMPITHITFLIACLAIAGVPPFAGFFSKEEILL"
     assembly_gap    265..378
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <379..907
                     /locus_tag="PK977_07550"
     CDS             <379..907
                     /locus_tag="PK977_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRF18006.1"
                     /translation="HAHGEKHGEGGFVMMLPLVILGIGAAAAGFIPFGEFVSSDGRPL
                     ESHFHLQFSILPVALGLAGILLAMWLYYKQNDKPAKLASSLGGLYKAAYHKFYIDEIY
                     LFVTKKILFNLVGRPAAWFDRNVVDGFINLTGNTTQAVSEGIKKVQSGKVQQYAIYFL
                     GGVIVLAVLFIYIWK"
     gene            930..>1550
                     /locus_tag="PK977_07555"
     CDS             930..>1550
                     /locus_tag="PK977_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF18007.1"
                     /translation="MNLLLLIGAPLLTALAVLLSRNKQQVKWISLAGSAVQLALAFAL
                     LFAFRQERAAGNNAQMLFEQHYKLFESLNINFHFGVDGISVAMILLTSFVVIAGVLVS
                     WNVEKMTKEFYFLLLLLGLGAYGFFISLDLFILFFFLEISVIPKYLLIGIWGSGKKEY
                     SANKLALMLMGGSALILVGMLGLYFTTGYSFDIMTLSKANIPAATQN"
     assembly_gap    1551..1569
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1570..>2035
                     /locus_tag="PK977_07560"
     CDS             <1570..>2035
                     /locus_tag="PK977_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF18008.1"
                     /translation="VGFGVFTALFPLHTWVPDGHSSAPTAGSMFLAGISMKLGGYGCL
                     RVATYLLPEGAKEYANIIIVLSAIAILYGAFATMMQKDLKYINAYSSVSHVGFSLLGI
                     GMLTQTAITGALMQMVSHGVMTALFFAVIGMVYDRTHTRMVAEMGGLMKMMPF"
     assembly_gap    2036..2058
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2059..2428
                     /locus_tag="PK977_07565"
     CDS             <2059..2428
                     /locus_tag="PK977_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18009.1"
                     /translation="ATVFFIVGLCSLGLPGFSGFVAEMTVFMGSWQNDDAFHRIATIA
                     AAMSIVVTAVYILRATGKTMMGPVVNAEHLQLGDAKWNEKLAAIILVAGIVAIGIAPG
                     WLNDLLKPAAEEIMNRIAGK"
     gene            2441..3841
                     /locus_tag="PK977_07570"
     CDS             2441..3841
                     /locus_tag="PK977_07570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRF18010.1"
                     /translation="MNEFLTLMKSELVLTGIIFLLLFIKIGKGMKNESLLSLIQLLLL
                     LKFIFSFFFNKEGNLFGDMFYTTPLIAFQKSILNLGVYLISLLCSDWLKKTQHLPEFF
                     LLMLSALLGMFLMISSGNLLIFYLSLELATIPVAAMANFDLERKRSSEGAMKMILSSA
                     FSSGILLFGISLIYGSTGSISLPAISQLLNGSPLQILAFVFVFTAFAFKLSIVPFHLW
                     TADVYEGSPIAVTSFLSVISKGAVAFIFITVLYKVFTPMLEIWYWMIVILAIATMVIG
                     NLFALRQQNIKRFLAFSSIAQVGFILVGISNADYLGIASVVYFVLIYVFSNLAAFGVA
                     AVIAEKTGKENIDDYKGLYKTNPFLTWIMALALFSLAGIPPTAGFFGKLFLLTAGGAR
                     AEYWFILVAALNMIISLYYYLRVVRAMFMDKNDNPVEPVKTAPVVKMALIICGGGIVL
                     VGLLSWIFEYIKTLQY"
     gene            3844..4227
                     /locus_tag="PK977_07575"
     CDS             3844..4227
                     /locus_tag="PK977_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18011.1"
                     /translation="MALNKNHEFEELDGVKCGIVEKNVKPERVNFLKELLEFNGYTVI
                     VVPSPAPKAAPAPKPAEGEAAAPPPPPPAPETFTVGVTDYTFNAVNAIFGRMLKTKDG
                     HIVTLAYWQQKEAASNDEIPYYEHQ"
     gene            4379..7138
                     /gene="ppdK"
                     /locus_tag="PK977_07580"
     CDS             4379..7138
                     /gene="ppdK"
                     /locus_tag="PK977_07580"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRF18012.1"
                     /translation="MATKTKAGKYVYFFGGGKADGNESMKNLLGGKGANLAEMAGHPK
                     LKLPVPPGFTVTTEVCTYYYANRKTYPKILKAQVQEALAKMEKLTGKKFGDAANPLLL
                     SVRSGARRSMPGMMETVLNVGLNETTIKGIIAQSGDERFAYDAYRRLIMMYADVVMEK
                     AAGIEAKNGKGIRKVLDEKLEHIKHSKGYKSDTELTADELKDLVKDYKKTVKKVLGKE
                     FPDDPYEQMWGGIGAVFASWNGKRAIEYRRIEKIPHEWGTAVNVQAMVFGNMGDDSCT
                     GVAFTRNPGNGENKFYGEYLVNAQGEDVVAGIRTPAPVNEYSKNAQSEHFTTLEKLMP
                     KLYKELFEYQRRLEKHYKDMQDIEFTIEKGKLYMLQCRVGKRNGVAAVRMATEMYKEK
                     LIDIKTAVMRVGPNQLVELLLPMLDPKAELTAPVIAKGLPAGPGGAKGRVVFSSADAV
                     AWAAKGEKVILVREETSPEDVDGMHKAQAILTTKGGMTSHAALVARGWGKCCIVGCGD
                     IEIHSDGKNFHAKNGVVIKEGDWISLNGTKGLVYEGSMALVDIDIDKNQSYKDLMKLV
                     DKTKQIGVRANAETPEDALHAVKFGAEGIGLFRTEHMFYGEGSEEPLFQLRKMIVSKT
                     EKERREALNGLFKYVKKDVKDTLEVMKGHPVTIRLLDPPLHEFVPHDKEKLRQLSKEL
                     GISMGMLNRRVLALHENNPMLGHRGVRLGISYPEITEMQVRAIFEATAELLKAGKKAL
                     PEIMVPVTVIWHELRHQKEIVDRVYKEVCEKMGMRKIPYLYGTMIETPRAALRAEFMA
                     KAADFFSFGTNDLTQMSFGFSRDDIGGFLPKYLDLKILPDDPFQTIDQEGIGQLIKAG
                     TERGRITKPNLKVGICGEHGGDPDSVKFCHRIGMNYVSCSPFRVPIARLAAAQAAIES
                     PRKKK"
     gene            7234..7683
                     /locus_tag="PK977_07585"
     CDS             7234..7683
                     /locus_tag="PK977_07585"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HRF18013.1"
                     /translation="MEIKNLFDPAVKQELVDRIHTLTPQTERKWGKMDVAQMLAHLQA
                     PMGVALGTHSLKANWLMKLIMPLFKKALYDEKPWKQGLPTDKTFITAGKPKNFEEEKT
                     KLLDMLNRFSENNMVNDKHPIFGKLTKEQWSKATWKHFDHHLKQFGA"
     gene            7851..9224
                     /locus_tag="PK977_07590"
     CDS             7851..9224
                     /locus_tag="PK977_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRF18014.1"
                     /translation="MKTIIEPFRIKSVEPIYFTTKEERKQILAKAEYNSFRIHANDIL
                     IDLLTDSGTSAMSSNQWAGIMQGDESYAGSNSFFRFEDSVRSITHMPYIIPTHQGRAA
                     EKIIFTILGGKGKYFISNTLFDTTRANIEFSGAEGIDCLCEEGKHPTIPAPFKGNMDV
                     EALRKVIKEKGAENIPMVIITVTNNSGGGQPVSMQNIKDVRTVCDEYKIPLFIDACRF
                     AENSYFIKLREQGYADKAVSEIAQELFSYADGCTMSAKKDAFANIGGFLAMKDLDLAV
                     RCRNLLIITEGFTTYGGLAGRDLEAIAIGLQEVMDENYLQYRIRSIEYLTNKLIDAAV
                     PVMQPAGGHAVYLDAKAFLPHIRVEQYPGQSLVCALYEEGGIRTVEIGSLMFGKYDEN
                     GKLVPAQMELVRLAIPRRVYTQSHIDYVAEVIIEVFNRRNELRGLVITEETPLLRHFT
                     AKLKPAQ"
     gene            9276..10661
                     /locus_tag="PK977_07595"
     CDS             9276..10661
                     /locus_tag="PK977_07595"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HRF18015.1"
                     /translation="MKHNIYKSWAEPYKIKMVELLKMTTAAQRKKALQEAGFNTFLLK
                     SEDVYIDLLTDSGTSAMSDRQWAGLMLGDEAYAGSRNFYHLEETVKEFYGYKYLVPTH
                     QGRGAEHILSKIMIKKGDIIPGNMYFTTTRLHQELAGGKFEDIIIDEAHDPENEFPFK
                     GNVNLEKLEALIKKHGAKKIPYVSIATSVNMAGGQPISIQNLKELRSLTKKHGIKIIH
                     DMTRVAENAHFIQKREKGFERKSIRQIVKDICNLTDGATMSAKKDALVNIGGFLAIND
                     WDVFEEARNMVVVYEGLHTYGGLAGRDMEAMAIGIKESVNDDHIHARVGQVVYLGEKM
                     MDFGVPIVKPIGGHGIFVDAKKFLPHIPQDEFPAQTLAAEVYLDSGVRTMERGVVSAG
                     RKANGENYYPKLELVRFTIPRRVYTQAHMDVIAESTARVYERRHKIRGLKMIYEPKYL
                     RFFQARFQQLK"
     gene            complement(10663..>11092)
                     /locus_tag="PK977_07600"
     CDS             complement(10663..>11092)
                     /locus_tag="PK977_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRF18016.1"
                     /translation="LSLTCKTAIKAVIYLASKFETGEKAGIKEIAEFIDASEHTVGKM
                     LQVLVKEEVINSVKGPNGGFFITEKQKNQPIINIVEAIDGKEVFKQCGLGFSKCSSAH
                     PCPLHDDYKVVRDLFEKMCREKKVTDLCEPVTSGLAYLFG"
BASE COUNT         3210 a         2299 c         2507 g         2920 t
ORIGIN      
        1 tgggtgtagc gaaatatggc gctgaacccg cattaggtta cacggcttcc atgttccacc
       61 ttttcaccca tgccatgttt aaagccttgc tgttcctcgg tgccggtgct gttatccatt
      121 atgtgcatag taatgaaatg aatgatatgg gcggactaag aaaatatatg ccgatcaccc
      181 atatcacctt tttgattgcc tgccttgcta tagctggtgt tccgccattt gccggatttt
      241 tcagcaaaga agaaattctg ttggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnntc atgctcatgg agaaaagcat ggcgaaggtg gttttgtgat
      421 gatgctgcca ttagtaatat taggaatagg cgctgctgca gccggcttca tcccatttgg
      481 cgaatttgta agttccgatg gccggcccct tgaatctcac tttcatttgc aattttcaat
      541 cttacccgta gcattaggac ttgccggcat attgctggct atgtggcttt attataaaca
      601 aaatgataaa ccggctaaac tggcttcttc attgggcggt ctttacaagg cagcttatca
      661 caaattttat attgacgaga tatatctctt tgtaacaaag aaaatattat tcaacctggt
      721 aggcaggccg gctgcatggt ttgacagaaa tgtagtggat ggatttataa acctgaccgg
      781 taataccaca caggctgttt cagaaggaat aaaaaaagta caatcaggaa aagtacagca
      841 atatgccatt tacttcctgg gtggggtaat tgtgctggct gtattgttca tttatatatg
      901 gaaatagaac tgaagacaaa atagtattga tgaacctttt attactcata ggagcccctt
      961 tactgactgc actggctgtg ttgctttccc gcaacaagca acaggtaaaa tggatatcgc
     1021 tggccggctc cgctgtgcaa ctggcattgg cctttgctct tttatttgct ttccggcagg
     1081 aaagagcggc cggcaacaat gcacaaatgc tctttgaaca gcattacaaa ttattcgagt
     1141 cgcttaatat caattttcac tttggcgtgg atggtatttc tgttgcgatg atattactga
     1201 cctccttcgt cgtaattgcc ggggtacttg tatcctggaa tgtagagaag atgaccaaag
     1261 aattttattt ccttctgctg ctgctgggac tcggcgcata tggtttcttt atttcgcttg
     1321 atctgttcat acttttcttc ttcctcgaaa tttctgtaat tccgaaatat ctcttaatcg
     1381 gcatttgggg aagtggcaaa aaggaataca gtgccaataa actggcgtta atgctgatgg
     1441 gtggttccgc cctgatactg gtgggcatgt tagggcttta tttcaccacc ggttacagct
     1501 ttgatataat gacattatca aaggcaaaca ttccggctgc cacacaaaat nnnnnnnnnn
     1561 nnnnnnnnnt gtgggctttg gcgttttcac agcgttgttc cctttacata catgggtacc
     1621 cgatggtcac tcctctgccc ctactgccgg ttccatgttc ctggcaggta tttcgatgaa
     1681 actgggaggt tatggctgtt taagagtggc tacctacctc ttacctgaag gagcaaaaga
     1741 atatgccaat atcatcatcg tactttctgc cattgctatt ttatatggtg ctttcgctac
     1801 gatgatgcag aaagacctga aatatatcaa tgcttactct tccgttagcc atgtgggctt
     1861 cagtttgctc ggtattggta tgcttacaca aacagccatt accggcgcat taatgcaaat
     1921 ggtatcacac ggggttatga cagccctttt ctttgccgtt ataggaatgg tgtacgacag
     1981 gacacataca cgaatggtag ccgagatggg cggactaatg aaaatgatgc cttttnnnnn
     2041 nnnnnnnnnn nnnnnnnntg ccaccgtatt ctttatagtg ggtctatgtt ctttaggact
     2101 tccagggttc agtggctttg tagcagaaat gaccgtcttt atgggctcct ggcagaatga
     2161 tgatgcgttt caccggatag ccactattgc cgcagccatg tcgattgtag taacagctgt
     2221 atatatatta agggcaacag gtaaaaccat gatggggcct gtagtgaatg cagaacattt
     2281 gcaattgggt gatgccaaat ggaatgaaaa attagcagcg atcatcctgg ttgcaggtat
     2341 agtagcgatc ggtattgccc cgggctggct gaatgatctg ctgaaaccgg ctgctgaaga
     2401 aataatgaac agaattgctg gaaaataaaa taatgaccgg atgaatgagt ttctcacctt
     2461 gatgaaaagt gaactggtgc ttaccggcat catcttcctt cttctcttta tcaaaattgg
     2521 taaagggatg aagaatgaat cactgctttc cctgatccag ctgctgcttt tactgaaatt
     2581 catattcagt ttctttttca acaaggaggg caatctcttt ggtgatatgt tctatactac
     2641 accattgatt gctttccaga aaagcatttt aaatctcggc gtttacctga tctcattatt
     2701 gtgttccgac tggctgaaga aaacacagca cctgccggaa ttcttcctgc tgatgttatc
     2761 tgcattgctg ggtatgttcc tgatgatctc cagcggcaat ctcctgattt tctatttatc
     2821 gctggaactg gcgaccatac ccgtagccgc tatggctaat ttcgacctgg agagaaaacg
     2881 ttcatccgaa ggagccatga agatgatcct gtcatctgct ttctcctccg gcatcttatt
     2941 gtttggcata tcattgatat acggcagtac tggttcgata agcttaccgg ccatcagcca
     3001 gttacttaat ggatctcctt tacagatact ggcttttgtt ttcgtattca ctgcctttgc
     3061 tttcaaatta tccattgtgc cattccattt atggacagcc gatgtatatg aaggctcccc
     3121 gattgctgtt acttcttttc tttctgtgat ctccaagggg gctgttgcat tcatctttat
     3181 cactgtcctg tacaaggtat ttacacccat gctggaaata tggtactgga tgattgttat
     3241 actggcaatt gccaccatgg tcattggcaa cctttttgcc ctgcgccagc agaacataaa
     3301 acgattcctt gctttctcct ccattgcaca ggttggtttc atcctagtgg gtatcagcaa
     3361 tgctgattac ctgggaattg cttcagtagt ttactttgta ttgatctatg tattttccaa
     3421 cctggctgct ttcggagtgg ctgcagtcat tgcagaaaaa acaggtaaag aaaatattga
     3481 tgactacaaa gggttgtaca agaccaatcc tttcctgaca tggattatgg cattggcctt
     3541 attctccctg gctggcatac cgccaacggc cggtttcttt ggtaaattat ttttattgac
     3601 agcaggtggc gccagggctg agtattggtt catcctcgtt gcggcattaa acatgatcat
     3661 ttcgctttac tattacttac gggtggtaag ggccatgttt atggataaga atgataaccc
     3721 ggttgaaccg gtgaagactg ccccggtagt caaaatggct ttgattattt gcggaggagg
     3781 tattgtgctg gttggcttgc tgagctggat atttgaatac ataaaaacat tgcagtacta
     3841 gttatggcac tcaataagaa tcatgaattc gaagaactcg acggtgtcaa atgcggcatt
     3901 gtagaaaaga atgtaaaacc tgaaagagtt aactttttaa aagagttact cgagttcaac
     3961 ggttatacag ttattgttgt tccttctcct gcacccaaag ccgcaccggc acctaagcct
     4021 gccgaaggcg aagcagcagc tcctccacca cctccaccgg ccccagaaac ttttactgtt
     4081 ggcgttacag actacacatt taatgcggtg aatgctattt ttggcaggat gttaaaaaca
     4141 aaagacggtc atatcgtaac gctggcttac tggcaacaaa aagaagcagc aagcaacgat
     4201 gagataccat actacgagca ccaatgagtc actattcgtt cttttatcag gagggaaacg
     4261 aatgtaaatc agcattttcc caatcaaaag tgataagaat caggatttcc gctgacctgc
     4321 gttttcatta atcaagtggc ttcgtcataa ctatgcaggg cataaaagct tgtatattat
     4381 ggcaacaaaa actaaggccg gaaagtatgt atacttcttc ggtggcggca aagccgacgg
     4441 aaacgaatca atgaaaaacc tcctcggagg caaaggcgcc aatctagccg agatggcagg
     4501 tcatcccaaa ctgaaattgc ccgttccccc gggatttaca gtaacaactg aagtttgcac
     4561 ctattactat gcaaacagga agacatatcc caaaatatta aaagcccagg tacaggaagc
     4621 actggcaaaa atggaaaagc taaccggaaa aaaattcggt gatgcggcta atcctttatt
     4681 gctctctgtt cgttccggcg cccgcagaag tatgccgggt atgatggaaa cggtacttaa
     4741 cgtaggcttg aatgaaacca ctatcaaagg aattattgcc caaagtggtg acgaacgttt
     4801 cgcttacgat gcttaccgcc gccttatcat gatgtatgcc gatgtggtga tggaaaaagc
     4861 agccggcatt gaagctaaaa atggtaaggg catccgtaaa gtgctggatg aaaaactgga
     4921 gcatatcaaa cacagtaaag ggtataaaag tgatacagaa ttaacagctg acgaactgaa
     4981 agacctggta aaagattaca agaaaaccgt gaagaaagta ttgggtaaag aatttccaga
     5041 tgacccatat gaacaaatgt ggggaggtat cggtgctgtg tttgccagct ggaatggtaa
     5101 acgagccatt gaataccgcc gcattgagaa aataccgcat gaatggggta ctgctgtaaa
     5161 cgtacaagcc atggtgtttg gtaacatggg tgatgatagc tgtacaggtg tagcctttac
     5221 ccgcaacccc ggtaatggtg aaaataaatt ctatggcgaa tacctggtaa atgcacaggg
     5281 tgaagatgtg gtagccggta tccgcactcc ggcaccggta aatgaatact caaagaatgc
     5341 acagagcgaa cattttacca ccctggaaaa gctgatgccg aagttgtaca aagaattatt
     5401 cgagtaccag cgtcgcctgg agaaacatta taaagacatg caggatattg agttcaccat
     5461 tgaaaaaggt aaactctaca tgctgcagtg ccgggtaggt aaacgtaatg gtgtagccgc
     5521 tgttcgtatg gctactgaga tgtataaaga aaaactcatc gatataaaaa ccgctgtaat
     5581 gcgggtagga cccaaccaat tagtagagtt gcttttacct atgctcgatc ccaaagctga
     5641 attaactgca cctgttattg ccaaaggctt accagcaggt ccaggtggag caaaaggccg
     5701 ggtagtattc tcttctgccg atgcagtagc atgggccgcc aaaggagaaa aagtgatcct
     5761 ggtaagagaa gaaacatctc cggaagatgt ggatggcatg cacaaagcac aggccatcct
     5821 gacaaccaaa ggtggcatga catcacatgc tgccctggta gcaagaggct ggggtaagtg
     5881 ttgtatcgtt ggctgtggtg atatagaaat tcacagtgat ggtaaaaact tccatgcaaa
     5941 gaatggggtt gttatcaaag aaggcgactg gatcagcctg aatggtacta aaggcctggt
     6001 gtatgaaggc agcatggcac tggttgatat tgacattgat aagaaccagt cctacaaaga
     6061 cctgatgaaa ctggtggata aaaccaaaca gatcggtgta agagctaatg ctgaaacccc
     6121 tgaagatgca ctgcatgccg ttaagtttgg cgccgaaggt attggcctgt tccgtaccga
     6181 acatatgttc tacggtgaag gaagcgaaga acccttgttc cagttgcgta aaatgatcgt
     6241 tagtaaaaca gagaaagaaa gaagagaagc actgaatggc ctcttcaaat atgtaaagaa
     6301 agatgtaaaa gatacgctgg aggtaatgaa aggtcacccg gttactatcc gcctgctcga
     6361 tccgcctttg catgagtttg ttccgcatga taaagaaaaa ctgcgccagc tcagcaaaga
     6421 actcggtatc agcatgggta tgcttaacag aagggtgctg gcattacatg aaaacaaccc
     6481 gatgctgggt caccgtggtg tacgtttggg tatcagttat ccggagatca cggagatgca
     6541 ggtaagggcc atttttgaag ctactgctga attactgaaa gccggcaaaa aagcattacc
     6601 tgagataatg gtacctgtaa ccgttatctg gcatgaactg cggcaccaga aagaaattgt
     6661 ggacagggtg tataaagaag tatgtgaaaa gatgggtatg cggaagattc cttacctgta
     6721 tggtacgatg attgaaacac caagagctgc cttaagagca gagttcatgg caaaagcagc
     6781 tgactttttc agtttcggca ccaatgacct tacacaaatg agttttggtt tcagccgcga
     6841 tgatatcggt ggtttcttac ccaaatattt ggatctgaaa atacttccgg acgatccgtt
     6901 ccagacaatt gaccaggaag gtatcgggca acttatcaaa gccggtactg aaaggggccg
     6961 catcaccaag ccgaacctga aagtgggtat ctgtggtgag catggcggag atccagacag
     7021 tgtgaagttc tgtcaccgta tcggtatgaa ctatgtaagc tgttctcctt tccgtgtgcc
     7081 catcgccagg ttggcggcag cacaggcagc cattgaaagc ccgaggaaga agaaataatt
     7141 tctcttgtta actgctaatg cccggaccgt tcatacatcc gggcattttt atttgcctat
     7201 attttccgac cttgccgacc ttaaacatta accatggaaa tcaaaaatct attcgatcct
     7261 gcagtaaaac aagaactcgt tgaccgcatc catacattaa ctccgcaaac ggaacgaaaa
     7321 tggggcaaaa tggatgtggc acaaatgctg gctcacctgc aagcccccat gggtgtggca
     7381 ttaggcaccc atagcctgaa agccaactgg ctgatgaaac tgatcatgcc gctgttcaaa
     7441 aaagctttgt atgacgaaaa gccctggaag cagggattac ctaccgataa aacattcatc
     7501 actgccggta agcccaaaaa ctttgaagaa gaaaaaacca aattgctgga tatgctaaac
     7561 cgcttctctg aaaataatat ggttaacgac aagcacccca tatttgggaa acttaccaaa
     7621 gagcagtgga gcaaagccac ctggaagcat tttgaccatc acctgaaaca gttcggcgcc
     7681 tgataaaagt catagccaaa aaagcctgcc ccttctactt ttgggcaggc ttttttattg
     7741 catgaacctc tggaaaaata tcatccgcaa accaagttaa ccgtttttct atccccggtc
     7801 cataacctca tttttccatc atcagctttc tgctgattaa aacattgcgg atgaaaacaa
     7861 tcatagaacc cttccggata aaatcggtgg aaccgatcta tttcactact aaagaagaaa
     7921 ggaaacaaat cctggccaaa gccgaataca actcgttccg tattcatgcc aatgatattc
     7981 tgatagacct gctgaccgac agcggcacca gcgccatgag cagcaaccag tgggccggca
     8041 ttatgcaggg cgatgaatct tatgccggca gcaacagctt tttccggttt gaagattcag
     8101 tacgtagcat cacgcatatg ccttacatca tacccaccca ccagggaagg gcagccgaaa
     8161 aaatcatatt taccatactc ggaggtaaag gaaaatattt tatcagcaat accttatttg
     8221 ataccacccg tgccaatatc gagttctccg gtgcagaagg aatcgattgc ctttgcgaag
     8281 aaggcaaaca tcctacgatt ccggccccgt tcaaaggaaa tatggatgtg gaagctttac
     8341 gaaaagtgat aaaagagaaa ggcgctgaga atataccgat ggtgattatc actgtcacca
     8401 ataattcagg tggtggccag ccggtaagca tgcaaaacat caaagatgta cgaacagttt
     8461 gtgatgaata taaaattcct ttgttcattg atgcctgccg ttttgcagaa aattcttact
     8521 tcatcaaact gagggagcaa ggttatgctg ataaggctgt atcagaaatt gcacaggaat
     8581 tattttccta tgccgacggc tgtacgatga gtgctaaaaa agatgccttt gccaatatcg
     8641 gcggtttcct ggctatgaaa gaccttgatc tcgctgttcg ttgcagaaac ctcctgatca
     8701 ttaccgaagg ctttacaacc tatggtggcc tggcaggaag agatttggaa gccattgcca
     8761 tcgggctaca ggaagtaatg gatgagaact acctgcaata tcgcatacgc agtattgaat
     8821 acctgaccaa taaactgata gatgctgctg tgccggtaat gcagccggca ggcggacatg
     8881 ccgtatacct cgatgcaaaa gcatttttgc cacatatccg tgttgagcaa tatccggggc
     8941 aatcattggt atgtgccctc tatgaagaag gaggcatccg tactgttgaa ataggctcac
     9001 tgatgtttgg caagtatgat gaaaatggta aactggttcc tgcacaaatg gaactggtgc
     9061 gtttggccat tccacgaaga gtgtacacac aaagccatat tgattatgta gcagaagtca
     9121 tcattgaagt ctttaaccgg agaaatgaat taagaggatt agtgataacc gaagaaaccc
     9181 cgttgctgag acactttacg gcaaaactga aacctgccca ataagcaaca ataaaatatt
     9241 caataaatca attttcaata ttcaataatc aacaaatgaa acacaacatt tataaaagct
     9301 gggccgaacc ttacaagatc aaaatggtgg agttgttgaa gatgacaaca gcagcacagc
     9361 ggaaaaaagc cttacaggaa gcaggcttta atacattttt attaaaatcg gaagatgtgt
     9421 acattgatct gctgaccgac agcggtacct ctgccatgag cgatcgtcaa tgggccggac
     9481 taatgctggg agatgaagcc tatgctggca gccgcaactt ttatcacctg gaagaaacgg
     9541 taaaagaatt ttatggttat aaatatttag ttcctactca ccagggccgc ggggctgaac
     9601 atatcctgtc aaagatcatg atcaaaaaag gggatattat tcccggcaat atgtatttca
     9661 ctactacacg tctgcaccag gaactggccg gcggaaaatt tgaagatatc attattgatg
     9721 aagcccatga tcccgaaaat gaatttcctt ttaagggcaa tgtgaacctg gaaaaactgg
     9781 aagccctgat caaaaaacat ggtgcgaaga agattcctta cgttagcatc gccaccagtg
     9841 taaatatggc gggtggacaa cccatcagca tacaaaattt gaaggagtta aggtcattga
     9901 caaaaaaaca tgggatcaag atcatacatg acatgacaag ggtggcagag aatgctcact
     9961 tcattcagaa aagagaaaaa ggttttgaga gaaaatcaat ccgccagatt gtaaaagata
    10021 tctgcaacct taccgacggc gctactatga gtgccaagaa agacgccctg gtaaacattg
    10081 gaggttttct ggccatcaac gattgggatg tatttgaaga agcaagaaat atggtagtgg
    10141 tatatgaagg attacacacc tacggaggcc tggctggtcg tgacatggaa gcaatggcta
    10201 tcggtatcaa agaaagtgtg aatgatgatc atattcatgc cagggtagga caggtagttt
    10261 atctcgggga aaaaatgatg gactttggtg tgccaatagt aaagcctatc ggcggtcatg
    10321 gtatttttgt tgatgctaaa aaatttcttc cacatatacc acaggatgag tttcctgcac
    10381 aaacattagc cgcagaagtt tacctggatt caggtgtaag aacaatggaa agaggtgtgg
    10441 tttctgccgg cagaaaagct aacggcgaga attattatcc aaaactggaa ctggtccgct
    10501 ttactattcc ccgccgggtg tacacacagg ctcatatgga tgtgatcgct gaatccacag
    10561 caagggtata tgaacgccgt cataaaatac ggggattaaa aatgatctat gaacccaaat
    10621 acctgcgttt tttccaggca aggttccagc aattaaaata acctaaccga ataaataggc
    10681 taatccactg gtaaccggct cgcagaggtc ggttactttt ttttcacggc acattttttc
    10741 aaaaagatct ctcactactt tataatcatc atgtaaagga cagggatgcg cagaagagca
    10801 tttgctgaaa cctaatccgc attgcttaaa gacctccttt ccatcaatcg cttccacaat
    10861 gttgatgata ggctgatttt tttgcttttc tgtaatgaaa aaaccaccat taggtccttt
    10921 aacactgttg atcacttcct ctttcaccag cacctgcagc attttaccaa cggtatgttc
    10981 gctggcatca ataaattctg caatctcctt aatgcctgcc ttctctcctg tttcaaactt
    11041 tgaagccagg tagatcaccg ctttgatagc cgttttacag gtaagactta ac
//
LOCUS       DAORLS010000077        11067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3164_length_11067_cov_1.836905, whole genome shotgun sequence.
ACCESSION   DAORLS010000077 DAORLS010000000
VERSION     DAORLS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_11067_cov_1.836905"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK977_07605"
     CDS             <1..308
                     /locus_tag="PK977_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HRF18017.1"
                     /translation="ISAFDFGVAAEEDDTMDISKGRINDISNRYIVLMTPDGHFIRMS
                     KKLKDLVCCISGEEVDEACLDQLKRWREKILSSSAVHAPGNFADILNLVTSLQDNNQ"
     gene            351..785
                     /locus_tag="PK977_07610"
     CDS             351..785
                     /locus_tag="PK977_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRF18018.1"
                     /translation="MARIKIELPATFHFSCSMPVRITDINYGGHAGNDAILSIIHEAR
                     MQYLHHLGYTEMNLAGTGMIMADVAIEFKSELFYGDTVTASVVAGDISKIGFDLFYKL
                     EKQENAVQKTVALAKTGMICFDYEKKKIVPMPEEARLKLTAH"
     assembly_gap    855..883
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<884..1146)
                     /gene="aroE"
                     /locus_tag="PK977_07615"
     CDS             complement(<884..1146)
                     /gene="aroE"
                     /locus_tag="PK977_07615"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRF18019.1"
                     /translation="MFVLKKLGIDFNIVSRKKHDHSTLTYHDINKKIIADCPLIINTT
                     PLGMHPDTGSCPDIPYQFITDRHLLYDLVYNPAKTLFLQKGEGR"
     gene            complement(1257..1523)
                     /locus_tag="PK977_07620"
     CDS             complement(1257..1523)
                     /locus_tag="PK977_07620"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18020.1"
                     /translation="MRLFGLIGYPLSHSFSKKYFTDKFENEGIAGCFYELFPLQTIEA
                     LLPLLQKHPELEGLNVTIPYKQQVLAYLDESKIPAIASISPAEG"
     gene            complement(1523..2296)
                     /locus_tag="PK977_07625"
     CDS             complement(1523..2296)
                     /locus_tag="PK977_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HRF18021.1"
                     /translation="MNFNLTQIPERNKKPRQSGITMVMDKGLSIDEAKNFLSVAHPHV
                     DIIKLGFGTSFVTPNLKDKLEVYRSFDIPVYFGGTLFEAFLIRNQFDDYVNVCKEYGI
                     SYMEVSDGSITIPHAEKCGYIEKLTKHGTVLSEVGSKDATHIIPPYKWIELMKAELEA
                     GSSYVIAEAREAGNVGIYRGSGEVREGLVQEILTQIPGEKIIWEAPQKAQQLYFIELL
                     GCNVNLGNIAPTEVIPLEAMRIGLRGDTFNLYLDKKVTA"
     gene            complement(2400..3821)
                     /locus_tag="PK977_07630"
     CDS             complement(2400..3821)
                     /locus_tag="PK977_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18022.1"
                     /translation="MKDNPYREDREQLKELLLQYENLKNGRQHSFIEEDSFEKIIDYF
                     HEKEDIVKAMEAAETGAEQFPYSAMLLIKKADILLATRRYQEALDILEQASLFDNNDI
                     NIYILKTDAYLALDQQEKAVQLLEEALSQFEGEEKLELLFELADVYDDYEDFDKVFDC
                     LKMVLEEDPNNEEALYKICFWTDFTGRNEESIRLHQQIIDEYPYNELAWFNLAAAYQG
                     LKLYEKAIDAYQYAITIDEKFDYAYRNMGDAYIRLRKYKEAIEVLEKVNELAKPEDVI
                     FEAIGHCYDRLKNFGQARIYYRKASHLRPDDSKLYYKIACTYFNEGNWGSSLKQLETA
                     MKIHRLQPEYNLLAGECKIQQGLYKDAIQYFSNVVRMRPRNAAGWEALIRCLYKAEFF
                     EEALEQADAAQQATHGKPLFTYYKAAILFSAGKSKEALLQLEKALEASPKQLKKFVEL
                     NPAILQNQQVVDMLARYKRKKSI"
     gene            complement(4038..>4257)
                     /locus_tag="PK977_07635"
     CDS             complement(4038..>4257)
                     /locus_tag="PK977_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18023.1"
                     /translation="SKVDFFGRSVDFLPSPKVAKAATADTAINRMTASFLRSSLLVNM
                     VFGWFISYKNNDKRNYKTNRLSRTLVLH"
     assembly_gap    4258..4294
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4295..4502
                     /locus_tag="PK977_07640"
     CDS             <4295..4502
                     /locus_tag="PK977_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18024.1"
                     /translation="TSGSFSLRSGYSYRGNQVINAGAEKRYINLNATVKVQKGNTTYI
                     VPLKKKVVVANVKIDLGNRSLQRN"
     assembly_gap    4632..4692
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4693..5179)
                     /locus_tag="PK977_07645"
     CDS             complement(<4693..5179)
                     /locus_tag="PK977_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18025.1"
                     /translation="MKRTLYILSSLLLMLAVTEINAQRRLTEATISYDIVINTSDKEP
                     KAVDLLDGATSVIYLKGNSSRSEMISSLGVQSTIIDGKSGHVTVLKDYGDQKYMIKMT
                     PDNWKASNKKYEGISFTYTEEYKTIAGYKCQKAIGKMADSSTFTVYFTKDLVPVNKDF
                     QY"
     gene            complement(5298..7154)
                     /gene="yidC"
                     /locus_tag="PK977_07650"
     CDS             complement(5298..7154)
                     /gene="yidC"
                     /locus_tag="PK977_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRF18026.1"
                     /translation="MKFDRNTVIGFVILAGLFFGYFYFTNKQQSEYRKQQAEKQKREK
                     AIQDSINLVNKPKQDSLSRIADSVAKITKVGSFQAAIKDTTEKLVVTENELIKVTFTN
                     KGGQVKKVELKKFKGLDSNEVKLAASSFDKLGYAINTGNNTTANTAELYFNAAEPVKN
                     ADGSQTIAFTLQSSDSTASTAITHKFTIRPNDYMLDFTADLTGADKLLTQGNMNLTWQ
                     YAAAQQESDIDFEKQNTQVGYVKDGSFDYHTIGRRSDKNFDKEVKWLGVRQRFFFTIL
                     VAKNNFSNGKMEWTIPADSTKTIVQSTANMKVQLPVATTVTAPFSIYYGPSDYHILKK
                     YDMKFEKLVNLGQGMYAFVRPLNRFIILPIYDFFRGLVGNMGIAIALLTLFIRLLISP
                     LTYSSYLSGAKMKALRPEIAALKDKYGEDRQAMSMEQMKLFREAGVNPLGGCIPALLQ
                     IPIFFALFSFFNSEVSLRGAEFLWASDLAAFDDPIKFGFHIPLLGSHLSLFNITACIT
                     SFMISWYSMSMTPDQSNPVLKYMPYIFPVFLLFFFNRLPSGLTWYYTVSNLITLALQF
                     IIQNYIIDHNKILAKIEENRKKPKTKSKWQERVEQMQEQQKRMKEMQQKNKR"
     gene            complement(7250..>8236)
                     /locus_tag="PK977_07655"
     CDS             complement(7250..>8236)
                     /locus_tag="PK977_07655"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRF18027.1"
                     /translation="RRKIALFCNVKMEAVIEAADAPTIYEVPLLMMREKLDVISLKKL
                     NITNYNEPELGKWKEFLDKLKYPKSKVTIGLIGKYIELQDAYKSILESFIHAGAMNEC
                     QVQVINIHSEFITDDNVAEKLTGLDGLLVAPGFGHRGVEGKIVAVKYARENGLPFFGI
                     CLGMQMAVIEFARNVLGVKEAHSTEMDPDTKEPVIDLMEEQKKITAKGGTMRLGAYPC
                     EIKNSTLAYTIYSSNAISERHRHRWEFNNKYLDRFEQAGMIASGKNPGTDLVEIVEIP
                     GHPFFIGVQYHPELKSTVENPQPLFVHFIKAAKLYAEKKTENKNPLLQSEMI"
     assembly_gap    8237..8255
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8256..>8741)
                     /locus_tag="PK977_07660"
     CDS             complement(<8256..>8741)
                     /locus_tag="PK977_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRF18028.1"
                     /translation="CYVTEDGAETDLDLGHYERFLNIFTSQANNVTTGRIYQTVINKE
                     REGAYLGKTVQVVPHITDEIKRRMLQLGQGGEYDIVITEIGGTVGDIESLPFVEAVRQ
                     LQWELPEEDTLVVHLTLIPYLKAAKELKTKPTQHSVKMLSQEGVHPDIIVCRTEKQLT
                     PE"
     assembly_gap    8742..8866
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9174..9443)
                     /locus_tag="PK977_07665"
     CDS             complement(9174..9443)
                     /locus_tag="PK977_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18029.1"
                     /translation="MKQILTLFLFTALIGATACKNEKTEDKPAPAETETKTPAPQKVD
                     PGMKESTRGEFKVHSCTENCKDGQHVFVHGEDGHTCSEECMKTNG"
     gene            complement(9501..>10260)
                     /gene="proC"
                     /locus_tag="PK977_07670"
     CDS             complement(9501..>10260)
                     /gene="proC"
                     /locus_tag="PK977_07670"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRF18030.1"
                     /translation="GASIADGLIASGFSKPAELTVTRRNVGALSRFSGKGCKVHSDNR
                     KAVRESEIIILAVKPYNYADIIKEIKPLLNGKKHLVVSVITGVWIEQLQKAIGKPVPV
                     IRAMPNTAIAIQQSMTCLAHAGASAAQLNYVQELFNQLGRTVLIDEKLMDAATVLGAC
                     GTAYAMRYIRANIQGGIEIGFDAATASLIAAQTIKGAAELLLEKGTHPEQEIDKVTTP
                     KGCTIAGLNEMEHQGFSSSLIRGLVASYDKIVKG"
     assembly_gap    10261..10327
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10487..11047
                     /locus_tag="PK977_07675"
     CDS             10487..11047
                     /locus_tag="PK977_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF18031.1"
                     /translation="MQKKFFSLITILSFSTALFVACNNDGEKKPAEDTTATVKEEPVA
                     PPAEAPKDVVDVAIGSPDHTTLVAAVQAAGLVETLKGAGPFTVFAPTNAAFNALPAGT
                     VEGLLKPEKKADLTSILTYHVVPGAVKAADLKDGQKIKTVQGAELTVSIKDGKVMIDA
                     ANVTTADLTASNGVIHVIDAVVMPKK"
BASE COUNT         2889 a         2425 c         2166 g         3249 t
ORIGIN      
        1 ctatttcggc ctttgatttt ggtgttgctg cagaagagga tgacacgatg gatatttcga
       61 aaggccgtat caatgatatc agcaaccgtt acatcgtatt gatgaccccc gatggccatt
      121 ttatccgcat gtcaaaaaaa ctgaaagacc tggtatgttg catctccggc gaagaagtgg
      181 atgaagcctg tttggatcag ttaaaacgct ggagagaaaa aattttaagc tcttcggctg
      241 tacatgcccc gggaaatttt gcagacattc tcaacctcgt tacttccctg caggataaca
      301 accagtagta caactttctt tccctatttt tattcaaatg atttacaata atggcaagga
      361 taaaaattga actgccagcc acctttcact tctcctgctc tatgcctgtc cgcattaccg
      421 acatcaatta cggcggtcat gcgggcaacg atgcaatact atccatcata cacgaagcaa
      481 ggatgcaata cctgcatcat cttggctata cagaaatgaa ccttgcaggc accggcatga
      541 tcatggccga tgtagccatc gaattcaaaa gcgaattgtt ttacggtgat accgtaaccg
      601 cctctgtggt ggccggcgat atttctaaaa ttggttttga ccttttctat aagctggaga
      661 aacaggaaaa cgcagtgcaa aaaacagttg cacttgccaa aaccggtatg atctgttttg
      721 actatgaaaa aaagaaaatt gttcctatgc cagaagaggc aagactaaag ctaacagctc
      781 attaatccaa acacactttt attcattcca aatccgccag ctttcttccg cctgcagtat
      841 cagcatttct tcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcccct cacctttttg
      901 cagaaacaac gttttggccg ggttatatac caggtcgtac agtaaatgcc tgtcagtaat
      961 aaactggtaa ggtatatcgg ggcaactgcc agtatccgga tgcataccca acggagtggt
     1021 attgatgatt aagggacagt cggcaataat ctttttattg atatcatggt aagtcaacgt
     1081 ggaatgatca tgtttcttcc ggcttactat attaaaatca atccccagtt tttttaaaac
     1141 aaacaccaca gcagctgttg ctccaccgtt gcccagtacc agcgcctttt tgtgccagga
     1201 ttttagcagg gggattacac ttagttcaaa tccggttaca tcggtattgt aacctgtcag
     1261 ccttccgccg gtgatattga tgcaattgca ggaattttgc tttcatccag gtaagctaat
     1321 acctgctgtt tgtaaggaat ggtcacattc aggccttcca gttccgggtg cttttgcaac
     1381 aaaggaagta aggcctctat agtctgcagg ggaaagagtt cataaaagca accggctatc
     1441 ccctcattct caaatttgtc agtaaaatat tttttagaga aagaatgaga caaaggatag
     1501 ccgatcagcc cgaataagcg catcaggcag tcactttttt atccaggtaa agattgaagg
     1561 tatcaccgcg gagaccaatt cgcatggctt ccagcggtat tacttccgta ggagcaatat
     1621 tgccgaggtt tacattgcaa cccagtaatt caataaagta taactgttgt gctttttgcg
     1681 gcgcttccca gatgatcttt tcaccgggta tctgggtcaa tatttcctgc acaaggcctt
     1741 cccttacttc accgctgccc cggtaaatac ccacattacc ggcctctctt gcctccgcaa
     1801 tcacataact ggatccggct tccagttcag ccttcatcag ttcaatccac ttgtatggag
     1861 gaataatatg cgtggcatcc ttgctgccca cttcactcag caccgtacca tgctttgtca
     1921 gtttttctat atagccgcat ttctctgcat gaggtatggt gatggagcca tcactcactt
     1981 ccatgtaaga gataccatac tccttacata catttacata atcatcaaac tggttacgga
     2041 tcaaaaatgc ttcaaataaa gtgccgccaa aataaacggg tatatcgaaa ctgcggtaca
     2101 cttccagttt atctttcagg ttgggagtta caaatgatgt tccaaagccc aatttgataa
     2161 tatctacatg cggatgcgct acacttaaaa aattctttgc ttcatctata ctcaggccct
     2221 tgtccattac catggttatg cctgattgac ggggtttctt atttctctcg gggatctgtg
     2281 tcaggttaaa attcataaac tatcggtttg aaatgaaaag atcttgaaga acggcggcaa
     2341 aaatagggaa gaaacagcag agcgctgctg atatggataa tgcttctaaa atctacttat
     2401 cagatagact tctttctttt gtatctcgcc agcatatcca ccacctgctg gttctgcagg
     2461 atagcggggt tcagttccac aaacttcttc agttgtttag gcgaagcttc cagcgctttc
     2521 tccagttgca ataacgcttc tttgctttta ccggcagaaa aaagtatggc tgctttatag
     2581 taggtaaaga ggggtttgcc atgtgtggcc tgctgtgcag catcggcttg ttccagcgct
     2641 tcttcaaaaa attcagcttt gtacaggcag cggatcaacg cttcccatcc ggcggcatta
     2701 cggggccgca tccgcactac atttgaaaaa tactggatgg catctttata caagccctgc
     2761 tgtattttac actcgccggc cagtaaattg tattcgggct gcagccggtg aatcttcatc
     2821 gccgtttcta actgtttgag actgctgccc cagttgccct cattgaaata ggtgcaggca
     2881 atcttataat ataatttgct gtcatccggc cgcaggtggg aggctttccg atagtagatt
     2941 ctcgcctgcc cgaaattctt cagccggtca taacaatgac cgatagcctc gaagatcaca
     3001 tcttcgggtt tggccagttc attcactttt tccagtactt ctattgcttc cttgtatttg
     3061 cgcagtcgta tataggcatc gcccatatta cggtaagcgt aatcaaactt ttcatcaatg
     3121 gtaatagcat actggtaagc atctatcgcc ttttcgtata gctttaatcc ctggtaggca
     3181 gccgccaggt tgaaccaggc caactcgtta tagggatatt catcaatgat ctgctggtgc
     3241 aaccttatgc tttcttcatt acggccggta aaatcggtcc agaagcagat cttgtacaaa
     3301 gcttcttcat tgttgggatc ttcttccaaa accatcttca ggcagtcaaa taccttgtca
     3361 aaatcttcgt aatcgtcgta cacatcggcc agctcaaata atagttccag tttctcttcc
     3421 ccctcaaact gtgaaagcgc ctcttctaac aattgtacag ccttttcctg ctggtccaat
     3481 gccaggtaag catctgtttt caggatatat atattgatat cattgttgtc aaacaaagaa
     3541 gcctgttcca gtatatccaa cgcctcctgg tagcgacggg tggccagcaa gatatctgcc
     3601 tttttaataa gcagcatagc tgagtaagga aattgttcag cacctgtttc agcggcttcc
     3661 atagccttta cgatgtcttc tttctcgtgg aaatagtcaa ttattttctc aaacgaatct
     3721 tcttcgataa aggagtgctg gcgtccgttc ttaaggtttt cgtattgtaa aagcagttct
     3781 ttcagttgtt cccgatcttc ccggtatgga ttatctttca ttcgttccgg tggtttgatg
     3841 aataatgtcc gacgttcgga tgataaagag ccagagcagg cagcaatccc gatagttatc
     3901 gggactcccc tgaaactgtt ttattctcaa caagttaata taaaaaaatg ggtaagtaaa
     3961 gcccctaatt ttccccatat gtgcaaaata atggcaattt ttaagcaacc taccgtaaca
     4021 atttcctttc ttctgcgtta atgaagtact aaagttctgg ataaacggtt tgttttataa
     4081 tttcttttgt cgttattttt gtagctgatg aaccacccaa acaccatatt tacaagtaag
     4141 ctgctgcgca gaaaactggc tgtcatccta ttgatggctg tatcggctgt agctgctttt
     4201 gccaccttgg gtgatggtaa aaaatcaact gacctcccga agaaatccac tttgctgnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncacttc tggcagtttt tctttgcgtt
     4321 ctggttatag ctaccggggc aaccaggtta ttaatgctgg cgcagagaaa aggtatatca
     4381 atcttaacgc aaccgttaaa gtacaaaagg gcaatactac ctatatcgtt cctcttaaga
     4441 aaaaagtggt ggtggccaat gttaagatcg atttgggcaa ccgctcactt cagcgcaatt
     4501 aatcgctttt cgttcctccc atgctttcct cataggtcat gaccctgtat cctgatttag
     4561 gcagatcaaa ctttgcctgc tgtacaggat taaaattgat cgaggccacg gtataggtca
     4621 ctatcatatt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nntatattga aaatctttat ttaccgggac aagatctttg gtgaaataga
     4741 ctgtaaaggt gctgctgtcc gccattttac caatagcctt ctggcattta tatccggcaa
     4801 tcgttttata ctcttccgta taggtaaaag aaatgccctc atatttttta ttggaggcct
     4861 tccagttatc cggcgtcatt ttgatcatgt atttctgatc gccataatcc tttaatacag
     4921 taacatggcc gcttttacca tcaataatag tggattgtac acccagtgaa ctgatcattt
     4981 cggaacggct ggagttaccc ttaaggtaga tgacactggt agcgccatcc agcaggtcaa
     5041 ccgcctttgg ctctttatcg ctggtattaa taacaatatc atatgaaata gttgcttctg
     5101 tcagtcttcg ttgtgcattt atttcagtaa cagccagcat tagcaaaaga ctacttagta
     5161 tgtataatgt tcttttcatt gtattcctat ctcctgtata gtaaaagacg tgccaattta
     5221 gccattaagt tgcgtaaaaa gaaaaaccgg gctttatgag ttaagcccgg ttactcttaa
     5281 gatatcttta acgaatctca gcgtttattt ttttgctgca tttctttcat cctcttttgc
     5341 tgctcctgca tttgttctac tctctcctgc cattttgatt tggtcttggg ttttttacgg
     5401 ttttcttcga ttttggccaa tattttatta tggtcaataa tataattctg gatgatgaac
     5461 tgcagcgcca atgttatcag gttggaaacg gtataatacc aggtaagacc ggaaggcagc
     5521 ctgttaaaga agaaaagcaa gaacaccggg aatatataag gcatatactt cagtacgggg
     5581 ttgctctggt caggcgtcat gctcatgctg taccaggaga tcataaagct ggtaatacaa
     5641 gcggtaatat tgaataagct gaggtgactt cccagtaacg gaatatggaa accaaactta
     5701 ataggatcat caaaagcagc caggtcagaa gcccacaaaa actcggcgcc cctcaatgac
     5761 acttctgagt taaagaagct aaacagggca aagaagatgg gaatttgcaa taatgcagga
     5821 atacatccgc ccaaaggatt tacacctgcc tccctgaata atttcatctg ctccatactc
     5881 atcgcctgcc tgtcttcccc atacttatct ttcaaagcag caatctcggg ccgcagggct
     5941 ttcattttgg ccccgctcaa gtaacttgaa taagtaagcg gagagatcag cagcctgata
     6001 aataaggtaa gcaaggcgat agcgataccc atattgccga ccaaaccgcg gaaaaaatca
     6061 taaataggta aaatgataaa gcggttcagt ggacgtacaa aagcatacat accctggcca
     6121 aggttgacca gcttctcaaa cttcatgtcg tacttcttca gaatatggta atcagaaggc
     6181 ccgtaataaa tactgaaagg agctgtaacc gttgtcgcaa ccggtaattg tactttcata
     6241 ttggcggtgg attgcacaat agttttagta ctgtctgccg gaatggtcca ttccatttta
     6301 ccgtttgaaa aattattctt tgcaacgaga atggtaaaga agaaacgctg tcttaccccg
     6361 agccatttca cttctttatc aaaattctta tcgcttcttc tgccgatggt atggtaatca
     6421 aaacttccgt cttttacata gcctacctgg gtattctgtt tttcaaaatc aatatcagac
     6481 tcctgctggg cagctgcata ttgccaggtc aggttcatat tgccctgtgt cagtaattta
     6541 tctgctcccg ttaaatccgc tgtaaaatca agcatataat cattgggcct gatagtgaac
     6601 ttgtgggtga tggctgtgga agcagtgctg tcagaagact gaagggtaaa ggcaattgtt
     6661 tgcgagccgt ctgcattttt tacaggttct gcagcattaa aatacaactc agcggtgttg
     6721 gccgttgtgt tattgccggt attgatggcg taaccaagct tgtcaaatga tgaagcggcc
     6781 agttttacct cgttgctgtc gagccctttg aatttcttta gttctacctt tttaacctgt
     6841 cctcccttat tagtaaacgt aactttgatc agctcatttt ccgtcactac cagcttttca
     6901 gttgtatcct taattgctgc ctggaaactg cctactttgg tgatcttggc cacagaatct
     6961 gcaattcggc tcaaagaatc ctgctttggt ttgttgacca ggttgatgga atcctggatc
     7021 gccttttccc ttttctgctt ttcagcctgc tgcttgcggt attcggattg ctgtttattg
     7081 gtaaaataga aatagccaaa gaataatcca gccagtatta caaaaccgat aacggtatta
     7141 cggtcaaact tcatgctgtg taaaaattta aagaccgcaa agataacggg tttctggttg
     7201 ccagtcgatg gtttctgacg tcgctaaaca gcacccgttg ggatgcagat cagatcattt
     7261 cgctttgcag caacgggttt ttattctccg ttttcttttc tgcatacaat ttggccgctt
     7321 tgataaaatg cacaaataaa ggttgaggat tttctaccgt actttttaac tcagggtgat
     7381 attgaacccc tataaaaaag ggatgaccgg gtatctctac aatctctacc aggtctgttc
     7441 cggggttttt gccactggca atcatgccag cttgttcaaa acggtccagg tacttattat
     7501 tgaattccca gcgatgacgg tgcctttcgc taatggcatt actgctgtat attgtataag
     7561 ccagtgtact gtttttgatc tcacaggggt aggcacctag cctcatggtt ccccctttag
     7621 ccgtaatctt cttctgttct tccatcaggt caataacagg ttctttggta tcgggatcca
     7681 tttctgtaga atgggcttct tttactccca gcacattccg tgcaaactca atgacggcca
     7741 tttgcatacc caaacatatt ccgaagaaag gcaaaccatt ttcccgggca tattttacgg
     7801 ctactatctt tccctccaca ccacggtgac caaatcccgg ggctaccaat aatccatcca
     7861 ggcctgttag cttctctgca acattatcat cagtgataaa ctcactatga atgttgataa
     7921 cctgtacctg gcattcattc attgcaccag catgaataaa agattcaaga atagatttat
     7981 aagcatcctg cagctcaata tattttccaa tgagcccgat ggtcacttta cttttgggat
     8041 acttaagttt atccaggaat tctttccact tgcctaattc cggttcatta taattcgtga
     8101 tatttagctt tttgagactg atcacatcca gcttttcccg catcatcagc aaaggcactt
     8161 cgtaaatagt gggcgcatct gccgcttcga tcaccgcttc catctttaca ttacagaaca
     8221 aagcgatctt tctgcgnnnn nnnnnnnnnn nnnnnttcag gcgtaagctg cttttcagta
     8281 cggcaaacga taatatcagg gtgtacccct tcctggctca gcatctttac actatgctgg
     8341 gtgggttttg tcttcaattc tttggctgct ttcaggtaag gtataagggt aagatgcacc
     8401 acaagggtat cttcttccgg caattcccat tgtagctgac gtacagcttc tacaaaaggt
     8461 aaactttcga tatcaccaac tgttccccca atttccgtta ttacgatgtc atactcgccg
     8521 ccctggccca gttgcagcat tcttcgcttt atctcatcgg tgatatgagg taccacctga
     8581 acggttttac cgagataggc gccttccctt tctttattaa ttacggtctg gtaaatacgg
     8641 cctgttgtta cattattggc ctgcgaggta aatatgttca ggaaacgttc gtaatgacca
     8701 aggtcaagat ccgtttctgc tccgtcttct gtcacataac annnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctt tacctaatga
     8881 agaagtaacc ccccctgtaa caaaaatata cttggccata caaaatggtt attaatttaa
     8941 aacacctttg aaatgatctg ataaatgagc cttgtaaaaa tgaccagcgg aagaatactg
     9001 tacttcaaac cctgtgcagg aaaaactccg ggaggtcgta caaagctaaa agattttttc
     9061 cggctgctaa aatttcatat ccacagaaat aatggtattg gtggaaaaaa cggtttgcaa
     9121 aataaaaggc cggaacagtt tgtaccggcc tgtctctttt ggaagaaaaa gttttatccg
     9181 ttggttttca tgcattcttc agaacaggta tgtccgtctt ctccatgaac aaatacatgc
     9241 tgtccgtctt tgcagttttc ggtacaggaa tgaactttaa attctcctct tgttgattcc
     9301 ttcataccgg ggtccacttt ttgcggggca ggggttttgg tttctgtttc tgccggggcc
     9361 ggcttatctt ctgtcttttc atttttacag gctgttgcac ctatcaaggc agtgaagagg
     9421 aacaaggtta aaatttgttt catggttttt tgttttaaat actaaatata aaaatttagt
     9481 ccggcacttt gaaatagaat tcagcctttc acaatcttgt cataactggc aaccagccct
     9541 cttatcagcg aagagctaaa gccctggtgc tccatttcat tcagcccggc aatagtgcag
     9601 ccttttggtg tagttacttt atcaatttcc tgttcgggat gtgtaccctt ttccaatagt
     9661 aattctgcag cacccttaat agtttgagct gctattaatg atgccgtagc ggcatcaaaa
     9721 cctatttcaa taccgccttg tatattggcc cggatatacc gcatggcata agcggtaccg
     9781 caggcaccca gtaccgtagc agcatccatc agcttttcat cgatcagtac agtacgtcct
     9841 aattgattga acaattcctg tacatagttt aactgtgctg ccgaagcacc tgcatgtgcc
     9901 aggcaggtca tgctttgctg aatggcaatt gccgtattgg gcatagccct gataacaggt
     9961 acaggtttgc caatagcttt ttgtaactgc tcaatccata cccctgtaat caccgacaca
    10021 accagatgtt ttttaccatt cagaagcggt ttgatttctt taatgatatc tgcataattg
    10081 tagggcttaa cagccaggat gataatttca ctttccctga ctgctttcct gttatcgctg
    10141 tgtactttac aaccttttcc gctgaaccgg cttaaggcac caacatttct gcgggtaaca
    10201 gtaagttctg ccggtttact aaaaccgctt gctatcaggc catcagcaat agacgctccc
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnttg gagtaaagat aagtctataa aaaaattctt atacgtttaa gcatcctgcc
    10381 ccaaaaaaaa gaaaagattc cacaaacaaa tcaatgtgag aaatatttat acccggttga
    10441 aaaccttttg aacagaatgg atgtttacca gcacaaaata atttatatgc aaaagaaatt
    10501 cttctcattg atcactattc tcagttttag tactgctttg tttgttgcct gtaataatga
    10561 cggcgaaaag aaaccggcag aagacaccac tgctactgta aaagaggagc ctgtggcacc
    10621 gccggcagaa gctcccaaag atgtggtgga tgttgccatt ggttcaccgg atcataccac
    10681 actcgttgca gccgttcagg cagccggact tgttgaaaca ttaaaaggag ctggtccatt
    10741 tactgtattt gcacccacca atgctgcttt caatgcctta ccggccggta cagttgaggg
    10801 cttgctgaag cctgaaaaaa aagctgatct taccagcatt cttacttacc atgtagtgcc
    10861 cggcgctgta aaagcagccg atctgaaaga tggccaaaaa attaaaacgg tacagggtgc
    10921 ggaactgact gtatcgatca aagatggtaa agtgatgatt gatgctgcta atgtaacaac
    10981 tgctgactta actgccagca atggagtaat ccatgtaatt gatgctgtag taatgccaaa
    11041 gaaataaaat ttttattgtc tggtttt
//
LOCUS       DAORLS010000078        11038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3180_length_11038_cov_2.028408, whole genome shotgun sequence.
ACCESSION   DAORLS010000078 DAORLS010000000
VERSION     DAORLS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3180_length_11038_cov_2.028408"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PK977_07680"
     CDS             <1..886
                     /locus_tag="PK977_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF18032.1"
                     /translation="YGKKNSVVTALKEISFEVDKGELFGIIGPDGAGKTSLFRILTTL
                     LLPEGGTASVDGFDVVKDYKAIRKRVGYMPGRFSLYQDLTVKENLDFFATIFNTSVEE
                     NYELVKDIYSQIEPFKDRRAGKLSGGMKQKLALSCALIHRPSVLFLDEPTTGVDAVSR
                     KEFWEMLQRLKKQGITILVSTPYMDEASLCDRVALVQSGKILSIDTPEQIVASFEKPL
                     LAVKSDNMLQLLSLLKQCEWVEDAYPFGEFHHVVMKGEFKADLSAYLSSQLSAFEMVD
                     TNPNIEDCFISLMKENDE"
     gene            879..>1332
                     /locus_tag="PK977_07685"
     CDS             879..>1332
                     /locus_tag="PK977_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF18033.1"
                     /translation="MSNDKVIIADKLTKRFGDFTAVDAISFEVNKGEIFGFLGANGAG
                     KTTAMRMLSGLSMPTSGKAIVAGFDIYRENEQIKRNIGYMSQKFSLYEDLTVKENIRF
                     YAGIYGKSNQFIKEKTERVLRQLHLSAEADKLVKSLPLGWKQKLAFSVA"
     assembly_gap    1333..1382
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1383..1635
                     /locus_tag="PK977_07690"
     CDS             <1383..1635
                     /locus_tag="PK977_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF18034.1"
                     /translation="GGVDPVTRREFWHMIYEAAASGITVFVTTHYMDEAEYCNRVSIM
                     VDGRIDALDTPQQLKKTFSASSMDEVFLKLARKATRAAD"
     assembly_gap    1720..1763
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1764..2748
                     /locus_tag="PK977_07695"
     CDS             <1764..2748
                     /locus_tag="PK977_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18035.1"
                     /translation="NEVKNSRIVVWDQSKDAATTSLINQIEASNYFELDREIYSANEI
                     ETSFKKGKVKLAVIFPPHFNEDLLHFNKAQVQLIADASDPNVANTLTNYASAIIMDYQ
                     DRITNERKLPYTITTEMRMLYNPELKGAYNFVPGVMAMVLLLVCTMMTAITIVREKEM
                     GTMEVMLVSPMKPFRIVLAKAVPYLMLSAVNITSILLLSVYVLDVPINGSLVLLVAES
                     LLFTLVSLSLGLLISSRAESQQTAMFISLVALFLPTVMLSGFMFPIENMPLPLRTVSN
                     IVPAKWYYSIVRSVMIKGVGLHAVWKETLILAGMFVFFLGLAIKNFKIRLA"
     gene            2745..3863
                     /locus_tag="PK977_07700"
     CDS             2745..3863
                     /locus_tag="PK977_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18036.1"
                     /translation="MRTLRFLLQKEFRQIFRDPSILRLIFVMPAIQLIVLPWAADYEI
                     RNIKLAVIDHDHSSYSRNLVNKVTASGYFSLTDYTGSYQEALSEIEHDRADIILEIPA
                     SFEKNLVKESEAALFMAVNAINGVKAGLGSSYLRSIIQDYNKEVRTEWIQFPRFSPET
                     NIEITSSNWFNPLMNYKVFMVPGMLVLLLTMVGANLTAINIVREKEIGTIEQINVTPV
                     KKAHFILGKLIPFWVLGLVVFTIGFLIARLFYGIVPFGSMLTIYVFAAVYLLAVLGLG
                     LLISTYADSQQQAMLISFFLMMIFVLMSGLYTSIDSMPDWAKAVTKANPVSYFIQVVR
                     MVVLKGSGLSDIKYQLLTIGGFAVLLNSWAILNYHKRS"
     gene            complement(3914..6103)
                     /locus_tag="PK977_07705"
     CDS             complement(3914..6103)
                     /locus_tag="PK977_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRF18037.1"
                     /translation="MSLRFKAIDGLTTKPTISVESATKITSIFNSNVFTLKTAREYLS
                     DEAYKSLQASIKAGHKIDRAVANQISNGIKAWAESKGVTHFTHWFQPLTGTTAEKHDS
                     FFTLKSDGTPIEQFEGDALVQQEPDASSFPSGGLRATFEARGYTAWDPTSPPFIMEIG
                     QGKTLCIPTIFVSYTGESLDYKAPLLKALDALNKAAVDVCNYFDKNITKVTPTLGWEQ
                     EYFVIDEAMFNARPDLIMTGRTVYGHSSAKNQQLEDHYFGSIPERVYTYMRDFENEAY
                     KLGIPLRTRHNEVAPAQFECAPIFEEVGVAVDHNSLLMDIMDRVARRHNLRVLLHEKP
                     FAGINGSGKHNNWSMATDTGVNLLAPGKTPKTNLMFLTFFVNTIKAVHDYADILRASI
                     ASAPNDHRLGANEAPPAIISVFIGQYLTKVLQDVKERVSDKMDETDESMLKIDIHKSI
                     PELLMDNTDRNRTSPFAFTGNKFEFRAVGSSANCANPMTVLNTIMAETLRQFKKEVDT
                     LIEKGEKKEIAIMHVIRQYIVASEKVLFEGDGYSEAWEKEAAKRGLPNVKTTPLALDA
                     MITEKAKELFEKNNIYSHSELEARHEIELEKYIKKVQIESRIMGEMATSHILPTSIRY
                     QNLLANNILQLKQAGLKESAYANQLQILEKISEHINKASDLVEKMIEARKVCNKIEDV
                     REKAIAYQEKVKDTFFDQIRYHVDKLELLVDDREWYLPKYREMLFLR"
     gene            complement(6228..7517)
                     /locus_tag="PK977_07710"
     CDS             complement(6228..7517)
                     /locus_tag="PK977_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18038.1"
                     /translation="MEHLLFIAWLVFFSWLVTRVKFFTQTGLSKPQLISFFLLKVMAG
                     IFYGWIGLYYGGLAKMQDTWSFHLGSLEEYNLLFKNPGEFFTNLFRDPYESSGVATFF
                     GSDNSYWNDLKGNLFIKLLSIFNVFSTGNYYINVIFYSFITFFGPLALYRVMSDVFPS
                     GKIPVLLSTFLVPSFIYWTSGLHKEGLIFTGISLITYAIYFGTKEKKWSIKRMASLVT
                     GLLLLLALRNFIFVIIIGAVIAWLLANRNPRHGLAIFAGTYLVFLTLFFTARYIDPRL
                     DFPQAVVDKQKAFLQLSPGNSTIPTTELSPTAGGFLKNIPQAVNLSMLRPFPSDVNHI
                     LSLAAAIEIDLLLFLFIIFLLWRKRESRSSNLILFCVFFSLSLLLAIGFTANNLGAIV
                     RYRSVIIPLLIIPVVARIDWKRIEIAFNNNIKNKNNI"
     assembly_gap    7602..7622
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7636..9894
                     /gene="purL"
                     /locus_tag="PK977_07715"
     CDS             7636..9894
                     /gene="purL"
                     /locus_tag="PK977_07715"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRF18039.1"
                     /translation="MEVTVKTAQQLRWTAEEFELIKQKLGRTPNFNELCAFSGMWSEH
                     CSYKNSIKWLKTLPREGGRMLVKAGEENAGLMDIGDGLGVVFKIESHNHPSAIEPFQG
                     AATGVGGIHRDIFTMGARPIAALNSLRFGNLDEDKTQHLLSGVVHGIGHYGNCFGVPT
                     VGGEIYFDECYHTNPLVNAMSVGIVKEGETVSATALGKGNPVMFVGSATGKDGIGGAS
                     FASADITAESVQDLPAVQVGDPFQEKKLLEACLEVIQTGAVVGMQDMGAAGIICSTAE
                     MSAKGGVGMRIDLDKVPTRQKNMKTWELLLSESQERMLLVAEKGREEEVKKVFDKWDL
                     PCSVIGEVTDDGILNFYMNGQLDASIPAHELVLGGGAPQYDREYKEPAYFAEISQFNI
                     DTVPVPGNLKKVAEEIIQLPSIASKRWVYNQYDSMVGTNNTSTNAPTDAAVVLVKGTT
                     KGIAVTTDCNSRYVFADPYKGAMIAVSEAARNIVCSGGQPLGVTNCLNFGNPYDPEVY
                     YQFVHAIKGMGDACRRFDTPVTGGNVSFYNQNPDGPVFPTPTIGMVGLLEDVTKKMTL
                     DYKKAGDHIYLLGESVNDIGSSQYLAKLCGVDHSPAPYFDLEKEHHLQQVVKGLIENG
                     DIQSAHDVSEGGLMTMLLEKGFSRGLGFDVSTIAESYTDGKALRSDAFWFGEAQSRVV
                     VTVAEEQLANFLRHMSKSELPFAKLGTVTNGEVIVDGQSWGAIELWKEKYDTSIENYL
                     AKEEAGAALSAI"
     gene            9891..>10190
                     /locus_tag="PK977_07720"
     CDS             9891..>10190
                     /locus_tag="PK977_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF18040.1"
                     /translation="MIGKDKIILLTGAAGFIGSYLLGFLNKQGYKNIIIADEFDEEDK
                     WFNFDTKEYMAKVEREKLFDWLEENKPVVSFVFHLGARTDTTEFDYSVHEKLNVAY"
     assembly_gap    10191..10258
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10259..10881
                     /locus_tag="PK977_07725"
     CDS             <10259..10881
                     /locus_tag="PK977_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF18041.1"
                     /translation="YGSGELGYNDDHELVEKLHPLNPYGVSKNEFDKWVLRQAQDNQP
                     PFWTGLKFFNVYGPNEYHKGRMASVIFHSFNQIRSTGKVKLFKSHRPDFNDGQQLRDF
                     IYVEDVAKVCCWLMNNNVSSGLYNLGTGKARSFEDLAKATFAAMGKQSVIEYIDMPED
                     IRDKYQYFTEANMSKLQKAGYTEKFCSLEEGVSDYVKNYLLPSVYY"
BASE COUNT         3178 a         2088 c         2518 g         3071 t
ORIGIN      
        1 ttacggaaag aaaaattctg ttgttacagc cttaaaggaa ataagctttg aggtagacaa
       61 aggtgaattg tttggtatca ttggtcccga cggcgccggt aagacttctt tattcaggat
      121 attgaccacc ctgcttttgc cggaaggtgg tactgccagt gtcgacgggt ttgatgtggt
      181 aaaagattat aaagcaatcc ggaaacgggt gggatatatg cccggcaggt tttctctgta
      241 ccaggacctg accgtaaaag agaaccttga tttctttgct acgatattta atacctctgt
      301 tgaggagaat tacgaactgg taaaagatat ttattcccag attgagcctt ttaaagacag
      361 gagagccggg aagttatccg gtggaatgaa acagaaatta gcattaagct gtgcactgat
      421 tcatcgacct tctgtactgt tcctggacga acctactacc ggtgttgatg ctgtttcccg
      481 aaaggagttt tgggagatgc tgcagcgatt aaaaaaacaa ggaatcacca tattagtgtc
      541 tacgccctat atggatgaag ccagtctttg cgaccgggta gcactggtgc aatcaggaaa
      601 aattttatct attgataccc cggaacaaat tgtggcttca tttgaaaagc ccttgctggc
      661 tgtgaaaagc gacaatatgc tgcaattatt aagcttgctt aaacaatgtg agtgggtaga
      721 ggatgcttac ccttttgggg agtttcatca tgtcgtcatg aaaggagagt ttaaggctga
      781 tctatctgca tacctgtctt cacaattgag tgcatttgaa atggttgata caaatccgaa
      841 tatcgaagac tgctttattt cattaatgaa agaaaatgat gagtaatgat aaagtgatca
      901 tagcagataa actgaccaag cgttttggtg attttaccgc agtggacgcc atttccttcg
      961 aagtaaacaa aggagaaata tttggctttt tgggtgcaaa tggtgctggt aaaacaacag
     1021 ctatgcgaat gttgtccggg ctctccatgc caacttccgg taaagctatt gttgcagggt
     1081 ttgatatata ccgggaaaat gaacagatca aacgaaatat cggctacatg agccagaagt
     1141 tttcgttgta tgaggatctt actgtaaagg aaaatatccg tttttacgcc gggatatatg
     1201 gaaagtcaaa tcagtttatt aaagagaaaa cagaacgggt tttaagacag cttcatttgt
     1261 ctgctgaagc agataaactg gtgaaatcgt taccgttggg ctggaaacag aaactggctt
     1321 tttcggtggc tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nngggtgggg tagacccggt tacccgaaga gaattctggc acatgatcta cgaggcggct
     1441 gcatcgggta ttactgtgtt tgttaccacg cattatatgg acgaggcaga atattgcaac
     1501 cgggtaagta ttatggtgga cggacggatt gatgctttgg atacaccgca gcagctcaaa
     1561 aaaacatttt cagcttcatc tatggatgaa gtgtttttaa aactggcaag aaaggctacg
     1621 agggcagcag attaatatga aacaattttt atcatttgta aaaaaagaat ttcatcacat
     1681 cttccgtgac ctgcggacca tgtttatatt attggctatn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnncaatgag gttaagaact ccaggatcgt tgtttgggac
     1801 cagtcaaagg atgcagcaac cacttcactg attaatcaaa tagaagccag taattatttt
     1861 gaattagata gggaaattta ttcagctaat gaaattgaaa cgagttttaa aaaaggcaaa
     1921 gtaaaactgg ccgtaatttt tcctccgcat ttcaacgagg acctgctgca ttttaataaa
     1981 gcacaggtgc aattgatagc agatgcctct gaccctaatg tagccaatac gctgaccaat
     2041 tatgcatcag cgataattat ggattaccag gaccggatta caaatgaaag aaagctccct
     2101 tatacaatca ccactgaaat gcggatgtta tataacccgg aattgaaagg agcttacaac
     2161 tttgttcccg gtgttatggc catggttttg ttattggttt gtacaatgat gaccgctatt
     2221 acaattgtgc gggaaaaaga gatgggaacg atggaggtaa tgctggtatc accaatgaag
     2281 cctttccgga ttgttttggc aaaagctgtt ccttatctga tgctatctgc tgttaacata
     2341 accagcatac tactgttgag tgtttatgtg ctggatgttc ccattaatgg cagcctggtt
     2401 ttactggtcg ctgaaagttt gttatttaca ttggtttccc tttcattggg attgctgata
     2461 tcatcccggg cagagtcgca gcaaacggcc atgttcattt cattggtagc cctgtttctg
     2521 ccaactgtca tgctgagtgg atttatgttt cctattgaga atatgccgtt gccgcttcgt
     2581 accgtttcca atatcgtccc ggccaaatgg tactacagta ttgtcagatc agtaatgata
     2641 aaaggagtgg ggctgcatgc agtatggaaa gagacgctga tactggctgg tatgtttgtc
     2701 tttttcctgg gacttgccat caaaaatttc aaaatcagac tggcatgaga acattacggt
     2761 ttttattgca gaaagagttc aggcagatat tcagggatcc gtctatactg cggctgatct
     2821 ttgtaatgcc cgcaattcag ttgatagtgc ttccctgggc ggctgattat gagataagaa
     2881 acattaaact cgccgtgatc gatcatgatc attcttctta ctcccgtaac ctggtaaata
     2941 aagtaaccgc ctccgggtat tttagtttga cggattatac cgggtcttat caggaggcgc
     3001 tgtcagaaat tgaacatgac agagcagata ttatcctgga aataccggct tcttttgaaa
     3061 agaacctggt aaaagaaagt gaagctgcct tgttcatggc tgtaaatgca attaacggag
     3121 tgaaggcggg tttgggcagt tcatacctga gaagtattat ccaggattac aataaggaag
     3181 taagaacaga atggatacag tttcccaggt ttagtccgga aacgaatatc gaaataacct
     3241 cctccaattg gtttaatccg ttaatgaact acaaagtatt tatggttccg gggatgcttg
     3301 tgctgttgct gacgatggtg ggggccaacc tgacggctat caatattgta cgggaaaaag
     3361 aaatcggaac aattgagcag atcaatgtga caccagtgaa aaaagctcat tttatactcg
     3421 gtaagttaat tcctttctgg gtattagggt tagtggtatt tacgattggt tttttgatag
     3481 cccgtttatt ttatgggata gtcccttttg gcagtatgtt aacgatttat gtttttgctg
     3541 cagtgtattt gctggcagtg cttggattag gcttattaat ttccacctat gctgacagcc
     3601 agcaacaggc catgctgatc tctttcttcc tcatgatgat cttcgtttta atgagtgggc
     3661 tgtacacttc tattgatagt atgccggact gggcaaaagc agttacaaaa gccaaccctg
     3721 tgagctattt catccaggtg gtacgtatgg tggtactaaa aggaagcggc ctgtcagata
     3781 ttaaatacca gttactgaca atcggtggat ttgctgtgtt gctcaactca tgggcaatac
     3841 tgaactatca taaacgcagc taataaaaag ccggcaattt tctgttgccg gctgatacat
     3901 acatagttct tgtttagcgg aggaataaca tctccctgta tttgggaagg taccattcac
     3961 ggtcatctac cagcaattcc agtttatcaa catggtagcg gatctggtca aagaaagtat
     4021 cttttacttt ctcctggtaa gcgatggcct tctctcttac atcttcgatc ttattgcaca
     4081 ctttcctggc ctcgatcatc ttttctacca ggtcgcttgc tttattgata tgctccgaaa
     4141 ttttttccag tatctgcaac tggttggcat aagcagactc tttcaacccg gcttgcttca
     4201 gctgaagaat attgttggcc agcaaattct ggtagcggat agaagttggc aggatatgac
     4261 ttgttgccat ttcacccatg atacggcttt caatttgcac cttcttgatg tatttctcca
     4321 gttcgatctc atgccttgct tccagttctg agtggctgta gatgttattt ttttcaaaga
     4381 gctccttggc tttttcagta atcatcgcat caagggcaag cggggtagtt tttacattag
     4441 gcaaacctct tttggcagcc tctttttccc atgcttcact gtagccatca ccttcaaaca
     4501 atactttttc gctggctaca atatactggc ggatgacatg cataatagcg atctcttttt
     4561 tctcgccttt ctcaatcaat gtgtctactt cttttttaaa ctgcctgagc gtttcagcca
     4621 tgatcgtatt taatacggtc atggggttgg cacagttggc agaagagcca acggcacgga
     4681 attcaaactt gttgccggtg aaagcaaaag gcgatgtccg gttacggtcg gtgttatcca
     4741 tcagcagttc cggaatgctc ttgtgaatat caatcttcag catgctttca tctgtttcat
     4801 ccattttatc tgaaactctt tcttttacat cctgcagcac tttggtaaga tattgaccaa
     4861 taaaaacgga aataatagca gggggagctt cattggcacc caaacgatga tcattaggtg
     4921 ctgaggcaat agatgccctt aatatatcag cgtagtcatg aacagctttg atagtattta
     4981 caaagaaggt caggaacatc aggttagtct tcggtgtttt accgggagcc agcaggttaa
     5041 cgcctgtgtc agtggccata ctccagttat tgtgtttgcc actgccgtta ataccggcaa
     5101 agggtttttc atgcaacaat actctaaggt tatgacgacg ggctacccta tccatgatat
     5161 ccatcaataa agagttgtgg tcaactgcta cacctacttc ttcgaaaatc ggggcacact
     5221 caaactgggc cggggctact tcattatgtc ttgtccgaag tggaattccc agtttgtaag
     5281 cttcattttc aaaatccctc atataggtat acaccctttc cgggatggag ccgaagtaat
     5341 gatcttccaa ttgctggttt tttgcagaag aatgtccgta aacagttctg ccagtcatga
     5401 tcagatctgg gcgggcattg aacatagcct catcaattac aaaatactcc tgttcccagc
     5461 ccaatgttgg ggtgaccttt gtaatatttt tatcaaagta gttgcagaca tccactgctg
     5521 ctttattcaa tgcatctaat gcttttagca aaggcgcttt atagtccagc gactcaccgg
     5581 tgtaggaaac aaagatagta ggtatgcaaa gcgttttgcc ttgtccgatc tccattataa
     5641 atggaggcga ggtaggatcc caggcagtgt aacccctggc ttcaaatgtg gctctgagac
     5701 ctccactggg aaatgaagac gcatctggtt cttgttgtac caatgcatct ccttcgaatt
     5761 gttcaatagg tgtgccgtcg ctctttaatg taaaaaacga atcatgtttt tctgccgttg
     5821 taccggtcaa tggctgaaac caatgcgtga aatgagtaac cccttttgat tcagcccagg
     5881 cctttatacc atttgaaatc tgattggcaa cggcacggtc aatcttgtga cctgccttaa
     5941 tagaggcctg caggctttta tacgcttcat cgcttaaata ctcccttgca gtctttagtg
     6001 taaagacatt gctgttgaat atggaagtga tcttggtagc actttctaca gaaatagttg
     6061 gtttagttgt aagtccatct attgctttaa atcggagtga catttgttat aattttccgc
     6121 aaagcaaata ttttgaactg attagaccaa ttttttatta aaaatgggga aaaaatatgc
     6181 ttttaatcaa gcgttgtttg caaaaatcaa taaaataata aggtttttta aatattgttt
     6241 ttattcttaa tattattatt gaatgctatc tcaattctct tccagtctat ccgggcaacg
     6301 acgggaatga tcaaaagagg gataataaca ctacgataac gcactatggc gccaagatta
     6361 tttgctgtaa aaccaatcgc cagcaataag gacaaagaga aaaatacaca gaaaagtatc
     6421 aggttactcg acctgctttc cctttttcgc catagcaaga atatgataaa caaaaagagc
     6481 agcaggtcaa tttcaatggc tgcagcgagt gacaggatat ggtttacatc ggaagggaag
     6541 ggcctgagca ttgacaggtt tactgcctgc gggatgtttt tcaaaaagcc gcccgccgtt
     6601 ggagagagtt ccgtagtcgg tatagtggaa ttgccgggag ataattgaag aaaggctttt
     6661 tgcttgtcca ccactgcctg cgggaaatca agtcttgggt caatataacg ggccgtgaaa
     6721 aataatgtca ggaaaacaag gtatgtgcct gcaaaaatag ccagcccatg cctggggttt
     6781 ctgttcgcca gtaaccatgc aatcacagca ccgatgatga tcacaaaaat gaaattacga
     6841 agtgccagca gaagcagtaa tcccgttacc aggctggcca tccgctttat actccatttt
     6901 ttttcttttg tgccaaaata aatagcatag gtaataagac tgataccggt aaaaatgagt
     6961 ccttctttat gcaatccgct tgtccagtat atgaaagatg gcaccaggaa ggtggataat
     7021 aatacaggta tttttccgga aggaaaaaca tcagacatca cccggtaaag agccagcggg
     7081 ccaaagaatg taatgaatga ataaaaaata acattgatat aatagttgcc ggtactaaat
     7141 acattgaaaa tagaaagcaa cttaataaaa aggtttcctt tcaggtcatt ccagtaagaa
     7201 ttatcgctgc caaaaaaagt tgcgaccccc gaagactcat acggatcacg gaaaagattg
     7261 gtaaagaact ctcccggatt cttaaaaagg agattatatt cttcaagact gcccaggtga
     7321 aagctccagg tatcctgcat ttttgcaaga ccaccatagt acaggcctat ccagccgtaa
     7381 aaaatgcccg ccatcacttt cagcaaaaaa aaactgataa gctgaggttt acttaatcca
     7441 gtttgggtaa aaaattttac ccgggtaacc agccaggaaa aaaagaccag ccaggcaata
     7501 aaaagaaggt gttccaatcc aatttcttta tagcgcaata atagccataa ttgtggtgaa
     7561 tgtcagcaca aagttgacaa gttatgaaga ggtactgccc cnnnnnnnnn nnnnnnnnnn
     7621 nnaatattct cttacatgga agtaaccgtt aagaccgcac agcaactgcg atggacagcc
     7681 gaagaatttg aattaattaa gcagaaactg ggacgaacac ccaactttaa tgaactctgt
     7741 gctttctcgg gcatgtggag tgagcactgc agttataaga attctattaa atggctgaag
     7801 acattgcccc gtgaaggcgg tcgtatgctg gtgaaagccg gtgaagaaaa tgcagggctg
     7861 atggatattg gtgacggttt aggagtggtg ttcaaaatcg aatcacacaa tcacccttca
     7921 gctattgaac ctttccaggg tgctgccaca ggtgtgggag gaatacaccg ggatattttt
     7981 acaatgggtg ccaggccgat tgcggcgctg aattcactgc ggtttggtaa cctggatgaa
     8041 gataaaacac agcatttatt atccggtgtt gtgcatggca tcgggcatta cggcaactgc
     8101 tttggagtgc caaccgtagg aggtgagata tactttgatg agtgttatca tacaaaccct
     8161 ttggtaaatg ccatgagtgt ggggattgta aaagaaggtg aaacggtatc tgccactgca
     8221 ttagggaaag gaaatcctgt gatgtttgtt ggcagtgcaa ccggcaagga tggtattggc
     8281 ggcgcttctt tcgcctcagc agatattacg gcagaaagtg tacaggactt acctgctgta
     8341 caggtcggtg atcctttcca ggagaagaaa ttactggaag cctgcctcga agtcatacaa
     8401 accggcgcag tggttggcat gcaggatatg ggggcagccg gtattatttg ttctacagct
     8461 gaaatgagtg ccaaaggtgg tgtgggtatg cggatcgacc tggataaagt gccaacccgg
     8521 caaaaaaaca tgaagacatg ggaattattg ctgagtgaaa gccaggaaag aatgttgctg
     8581 gtagcagaga aaggaagaga agaggaagta aaaaaagtgt ttgacaaatg ggatttgcct
     8641 tgctccgtta ttggtgaagt aacagatgac ggcatattga atttctatat gaatggtcag
     8701 ctggatgctt ccattccggc acatgaactg gtattgggtg gcggcgcccc gcaatatgac
     8761 cgggaatata aagaaccggc ttacttcgct gaaatcagcc aattcaatat cgatactgtt
     8821 cccgttcccg gaaacctgaa aaaagtggct gaagagatca ttcaattgcc atccatagct
     8881 tcaaaacgat gggtgtacaa ccagtatgac agcatggtgg gaactaataa tacttccacc
     8941 aatgcaccca cagatgcggc agtggtactg gtgaaaggaa caacgaaagg aattgcagta
     9001 acaacagatt gtaacagccg ttatgtgttt gcagaccctt ataaaggagc aatgattgcg
     9061 gttagtgaag ccgcccgaaa tattgtttgc agcggtggtc agccccttgg tgtaaccaat
     9121 tgcctgaact ttggaaatcc atacgatccg gaagtttatt accagtttgt acatgctatc
     9181 aaaggaatgg gtgatgcctg ccgtcggttt gacacacctg taacaggagg taatgtaagt
     9241 ttctacaacc aaaatccgga tggtccggta tttccaacac ctactatcgg tatggtaggt
     9301 ttattggaag atgtgaccaa aaaaatgaca ttggattata agaaagccgg tgatcatatc
     9361 tacctgctcg gagaatctgt aaatgatata ggttcatcac aatacctggc taagctttgc
     9421 ggggtggacc attcgccggc gccttacttt gacctggaga aagaacatca cctgcaacag
     9481 gtagtaaaag gattgattga gaacggagat attcaaagtg cccatgatgt aagtgaaggc
     9541 gggctgatga ccatgttatt ggagaaagga ttcagcaggg gattaggatt tgatgtaagt
     9601 acaatcgctg aatcatatac cgacggaaaa gctttacgca gcgatgcatt ctggtttggt
     9661 gaagcacaaa gccgtgtggt ggtaacagtg gccgaagaac agttagcaaa tttccttaga
     9721 cacatgagta aatcggagct gccttttgca aaactgggaa ctgtaaccaa tggtgaagtg
     9781 attgtagacg ggcagtcatg gggagctatt gaattgtgga aagaaaaata tgacacatcc
     9841 attgaaaatt atcttgctaa agaagaggca ggtgccgcat tgagtgcaat atgattggaa
     9901 aagacaaaat aatactactg acgggagctg ccggatttat tggcagttac ctcctgggct
     9961 ttttaaataa acagggatat aagaacatca tcattgcaga tgagtttgat gaagaggata
    10021 aatggttcaa ttttgacacc aaagaataca tggcaaaagt ggaaagggaa aaactgttcg
    10081 actggctgga agaaaataaa ccagttgtaa gttttgtttt tcatctgggg gcaaggacag
    10141 atactacaga gtttgactat tccgttcatg aaaaactgaa tgttgcctat nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
    10261 tatggtagcg gagaactggg gtataatgat gatcatgaac tggtggaaaa attacacccg
    10321 ttaaaccctt atggtgtttc taagaatgag tttgataagt gggtccttcg gcaagcgcag
    10381 gacaatcagc caccattttg gacagggttg aaatttttca atgtatatgg tcccaatgag
    10441 tatcacaaag gaaggatggc cagtgtgatc tttcattcgt ttaaccagat caggtcaacc
    10501 ggaaaagtga agctttttaa atctcatcgt cctgatttta atgacgggca acaactccgt
    10561 gattttatat atgtggagga tgtggccaaa gtctgctgct ggctgatgaa taacaatgtg
    10621 tcatccggct tatataattt aggaaccggt aaagcccggt catttgaaga cctcgccaaa
    10681 gctacttttg ccgcaatggg aaaacaatcg gtgattgaat acatagatat gcccgaagat
    10741 atccgggata aataccaata cttcacagag gccaacatgt ccaaactgca gaaagccggt
    10801 tacacggaaa aattctgttc attggaagaa ggggtgtctg attatgtcaa aaattatctt
    10861 ttgcccagtg tctactatta aaaagggagc aactttcgtt actccccttt acttaaaacc
    10921 agataatgaa aattttattt ctttggcatt actacagcat caattacatg gatcacacca
    10981 ttgctggcag ttaagtcagc agttgttaca ttggcaccat caatcattac tttaccat
//
LOCUS       DAORLS010000079        10999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3196_length_10999_cov_2.316520, whole genome shotgun sequence.
ACCESSION   DAORLS010000079 DAORLS010000000
VERSION     DAORLS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3196_length_10999_cov_2.316520"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PK977_07730"
     CDS             <1..760
                     /locus_tag="PK977_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF18042.1"
                     /translation="EMQITILPPFWKTWWFFTVLGILFIALLTYGINQRNKRKYAKKL
                     QQLENERQLKLERERISKDLHDNIGAYANAVLYNSELLEKETTGEKRKDLIADLKFAS
                     KDIITSLRETVWALKNEEYTAEDCLVRIKNFVHPFAKYYSHIQFPIQGDAPPGLILHY
                     TDALNLVRIVQEAVSNSIKHAKASVIAITSDTSENKWKLVVKDDGIGFNFNAMKEEDK
                     GNGLDNMEQRAAESGFKFCIESGMDTGTVITIIV"
     gene            763..1440
                     /locus_tag="PK977_07735"
     CDS             763..1440
                     /locus_tag="PK977_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18043.1"
                     /translation="MAIKLAIVDDRASNRNILKDKLLRYHQFEISLIAENGKDFLEKM
                     KTVPVEKIPSVVLMDLEMPEMDGVDAVAAGSYLYPSVKYVMLTIFDDEEKIFKAIKAG
                     AFGYLLKDESSDNIAEVLIQMHENGVGPISPGIAHKILQLVQNNSISLSQKTPHEKGK
                     TPFNLTEREQEILKLLMQGLLYKEIGSKLDISPNTAKKHVLNIYAKLHVNSKAQALAL
                     AYEKGLI"
     gene            1463..2038
                     /locus_tag="PK977_07740"
     CDS             1463..2038
                     /locus_tag="PK977_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18044.1"
                     /translation="MKTTIWVILLLIAASATAQSEVEIRNHYQDINKKITESKEQGYE
                     GSLYCNEWVANKNGKSWPAVGIYQETVQFWYDDDPNHLPASERNPKNVLMKVNISRKA
                     SALTTNEEYLFENGKLVFYFRHEGEEGNEWETRVYYNNKGSSFKSSVKANGKELTAKD
                     FLTDEYRDFKPKPLSIMAEGRKLQELFIKSM"
     gene            complement(2090..>4590)
                     /locus_tag="PK977_07745"
     CDS             complement(2090..>4590)
                     /locus_tag="PK977_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18045.1"
                     /translation="NEIIGYSTGQWAHVGVIKKGTTLTFYFNGAAVGTAVITPPVNAN
                     SYLSIGCRQNTAGSLMEFFNGKIDELRIYKRALSPNEMAQLADIPFMPDLLAYLPMNG
                     DANDKSSNSRNGSPYGNSQLTTDKYDNNNSAYSFTNPESGISLANTNTLDFVGQPFAI
                     SAWVRYSNIPAADFAIVAKHNCGVPNGYVLSVNNNIPRFYLSNGGWSMISASQAYNDN
                     KWHHLVATYDGIGSQKLYVDGELKASAASVVYNTPGSGAPIIIGDANGICGGATFSGS
                     IDEVKIYGAALDANQVTSLYKQSRGSGNALLFQPNSTNRVEITDEIPAAGIPPDLTFE
                     AWIKRNSSQPNQWLFAGNQIGNIMWGLDNNTMVFGTLGSAFAASEDISVINNNKWHHV
                     AVTYNNTTGLTSFYVDGLAKGAANGSYSFTGGNYAIGCKPGTNESYDGLLDEMRIWGA
                     VRSQNDIRDRMCQKLTNSEENLGLYFKFDEASLNRTYDVSSGFKTTVNGPQYVLSGAP
                     IGDRSAHDFVNPDATPNTSVIYPDPTKTNWFFRARPTTTGTITGLTVYQVLDEPLYQF
                     GILGAGGASHYFGVHVSGATTGATYNAIYVYAGNPLVSGYNEPTLKLYKRNDNSITDW
                     IDAGAALNTTNKVLVATGQSTEYILGSSGFGLPVTLLSFEAKKINATTVQLNWKTATE
                     INNKGFEVQRSFDGSYFVNIGFVNGAGNSDMMKEYITTDIPGKTGRVYYRLKQVDFDD
                     QAKLSQIVSVVFDKQGIVKVYPNPAQQKVTIEGADLYSRIQLFDAGGRMVREKTNNNQ
                     YLFTLNLEGLKSGIYLLQLGNSSGSQTLKLVIGN"
     assembly_gap    4591..4734
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4735..>4966)
                     /locus_tag="PK977_07750"
     CDS             complement(<4735..>4966)
                     /locus_tag="PK977_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18046.1"
                     /translation="VAYYPFNGNANDESGNGHDGTVSNVVLTTDRKNETDKAYAFNGS
                     TSSISAPAHADWSFNGGDFSIASWINVNIVNTS"
     assembly_gap    4967..5040
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5041..6932)
                     /locus_tag="PK977_07755"
     CDS             complement(<5041..6932)
                     /locus_tag="PK977_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRF18047.1"
                     /translation="MRTLLLLAWLSLSVVSYTQTLVAYYPFNGNPNDESGNGRNPTYV
                     GASLTTDRFGNANHAYEFSGMTSGHIEYSANGLPATNRTISLWFYTADVANRPGLLGY
                     GGNGSCGTTLFMALNLSGSGQYNVQGHCGANAVSFTYSSVPVNQWYHWVLTINGSVQK
                     IYVNGVLKSTDNTFSGNTATSGSAELSFGVIPYTNGLAPYIDGNVGYLIGKLDDIRIY
                     DAALSDAQVLQLYQNESTGLVAYYPFNGNANDESGSGYNGTVSGATLTSDRYGVSNSA
                     YQFNGIGSAITLNNSTNMDFVENSTGKPFSLSAWARLGTNSGNVELIVGKHFCGSYGG
                     YLLVTQNGTFSFWTAVNGNSWVQLTTSQNFLDDKWHHVVATYDGVNQKIYVDGLLKGT
                     LASAYNSPATGIPVKIGQPEGCGSSHIYNGKLDDIRIYNISLNDAQVLQLYQTESTGL
                     VAYYPFNGNANDESGNGYNGTVAGASLTTDRFNQSDKAYSFVWNGTSSDKIQVPGTSS
                     LNFNAGGFSISAWFKFSGTSNAGYNYPIVSKHNCGENSGYILMLYNDKLTFWIGGAGW
                     TPLSTTESYIDNNWHQVTAVYDGTNRFIYVDGVAKATDAVAYTITNAANIALGGYNGC
                     NGGFNG"
     gene            complement(7053..8609)
                     /locus_tag="PK977_07760"
     CDS             complement(7053..8609)
                     /locus_tag="PK977_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF18048.1"
                     /translation="MLQEYFKKYRDNIIGIDKEFETPYGLKKMVYADWTASGRAYRPI
                     EQRLQNDIMPLLANTHTETTVTGTAMTRAYEQAKHIIKQHVNAGPDDVLIFAGSGMTG
                     AVNKLQRLLGLRIPERIMDYVKPGRLPSHEELANSKVKIHSLFSNYLDIDPELKPVIF
                     VSHMEHHSNQTSWLETIADVEIIPNDENGNINLAGLEQLLEKYKERKNKIAAITGCSN
                     VTGIQTPYHKVAQLVHRHNGLCFVDFACSAPYVSINMHPSLEGGHLDAIFFSPHKFLG
                     GQGTPGVLIFHKSLYNNIIPDQPGGGTVTYTNPWKYHEYISDIEHREDGGTPPFLQGI
                     KAALAIKLKEEMGVENMLKREEELLKIIFEKLSSIPGVEILEGQNHKRLGVISFIING
                     THFNLVVKLLNDRFGIQTRGGCACAGTYGHMLLHVDQLHSYEILDEIHHGDLSCKPGW
                     IRMSVHPVMTDEEIHFILDAIEQLALHYNEWKQEYVYEPDTNEYHHQTFKEKTVNLVN
                     DWFHLPFQEF"
     gene            8814..9761
                     /gene="paaA"
                     /locus_tag="PK977_07765"
     CDS             8814..9761
                     /gene="paaA"
                     /locus_tag="PK977_07765"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRF18049.1"
                     /translation="MSVQDLEQIFQEKIDNEVKIEPKDWMPDAYRKTNLRQISQHAHS
                     EIVGMLPEGNWISRAPSLKRKAILLAKVQDEAGHGLYLYSAAETLGQSREEMINDLHS
                     GKAKYSSIFNYPTLTWADIGAIGWLVDGAAIMNQVMLTRTSYGPYARAMVRICKEESF
                     HQRQGFEALLVLSRGSAEQKAMCQDAINRWWWPALMMFGPRDTESTNSDQSMKWKIKR
                     KSNDELRQNFVDMIAEQIKTLGMKLPDDKLKWNEERKHYDFGEINWDEFWQVVKGNGP
                     CNKQRLDARRKAWENGEWVREAAAAYASKRAAKQKHIAA"
     gene            9772..10131
                     /gene="paaB"
                     /locus_tag="PK977_07770"
     CDS             9772..10131
                     /gene="paaB"
                     /locus_tag="PK977_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRF18050.1"
                     /translation="MNFIRKFYYGDIPEEKGGGKTDAKSSGHDWPLWEVFIRSKNGLD
                     HKHAGSLHAADAQMAIENARDVYTRRMEGVSIWVVESKYITASNPEEAGELYEPAVDK
                     IYRHPTFYNLPDEVNYM"
     gene            10131..>10584
                     /gene="paaC"
                     /locus_tag="PK977_07775"
     CDS             10131..>10584
                     /gene="paaC"
                     /locus_tag="PK977_07775"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRF18051.1"
                     /translation="MSALIDYTLHLADNALILGQRNSEWCGHGPILEQDIAITNISLD
                     LIGQSRSLYQYAAQLKADGSTEDSLAYLRTEREFKNCLLAEQSNGDWAQTMLRQFLFS
                     CYQFYLYKYLQSNPDKTIAAIAEKSLKEVSYHLRWSSEWVIRLGDGTEE"
     assembly_gap    10585..10595
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10596..10869
                     /locus_tag="PK977_07780"
     CDS             <10596..10869
                     /locus_tag="PK977_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HRF18052.1"
                     /translation="RMLTAIDELWRYTGELFIAAPYEIELNIDLASLKTEWDNKVKAV
                     MEEATLPVPEKLFMQTGGKTGTHTEQLGYILSDLQYMQRAYPGCEW"
BASE COUNT         3271 a         2305 c         2202 g         2992 t
ORIGIN      
        1 agaaatgcaa atcaccattc ttcccccgtt ctggaaaacc tggtggtttt tcacggtact
       61 gggtatactt ttcattgcat tgcttactta cggcatcaac cagcgaaata aaaggaaata
      121 tgctaaaaaa ttacagcaat tggagaatga aaggcaatta aaactggaaa gagaaagaat
      181 ttcaaaagac ctgcatgata acattggcgc ctatgcaaat gcagttttgt ataattcaga
      241 attattagaa aaagaaacca ccggggaaaa acgaaaagac cttattgccg atttgaaatt
      301 tgcctcaaag gatattatta cttcgctgcg tgaaactgta tgggctctaa agaatgaaga
      361 atataccgcc gaagactgtc ttgttcggat taaaaacttt gttcatccat ttgctaaata
      421 ttacagccat attcaatttc ctatacaggg agatgctccc cctggcctga tacttcatta
      481 tacagatgca ttgaatcttg tcagaattgt acaagaagct gtatccaaca gtatcaaaca
      541 tgcaaaagct tctgttattg cgataacaag cgatacctca gaaaacaaat ggaaattagt
      601 ggtaaaagat gatggtattg gttttaattt taatgccatg aaagaagaag acaaagggaa
      661 tgggctcgac aatatggaac aaagagctgc agaatccggc tttaaattct gcattgaatc
      721 cggaatggac accggcacag taataacaat cattgtctaa ctatggcgat taaacttgca
      781 attgtagatg acagggccag taatagaaat attctgaaag acaaactatt acgctaccat
      841 cagtttgaga ttagcctaat agcagaaaat ggaaaagatt ttttagaaaa aatgaaaaca
      901 gttcctgttg aaaaaattcc atccgtagta ttaatggatc ttgaaatgcc tgaaatggat
      961 ggtgtggatg ctgttgctgc aggatcctat ttatatcctt ctgtcaaata tgttatgctg
     1021 acaatttttg atgacgaaga aaaaattttt aaagctatta aagccggagc ctttggctac
     1081 ctgttaaaag atgaaagttc agataatatt gctgaggttt tgatccagat gcatgagaat
     1141 ggtgtaggcc ccatttcacc gggtattgcg cacaaaatat tacaacttgt tcaaaataac
     1201 tctatttcgc tatcgcagaa aacacctcat gaaaaaggga aaacaccctt taacctgaca
     1261 gaaagagaac aggaaatttt aaaactgctt atgcagggct tgctttataa agagataggc
     1321 agcaaactgg acataagtcc caataccgct aaaaaacatg tactgaatat ttatgccaag
     1381 ctgcatgtaa acagcaaggc ccaggcgctg gcccttgcct atgaaaaagg acttatctaa
     1441 attcatggaa ataaataaag tgatgaaaac aacaatatgg gtgattttgc tgctcatagc
     1501 tgcatcggca acagcgcaat cagaagtaga aattcgtaac cattaccagg acatcaacaa
     1561 aaaaatcacg gaatccaagg aacagggata tgaaggctca ctctattgca acgagtgggt
     1621 tgccaacaaa aacggtaaaa gctggccggc agttggtatt taccaggaaa cagtacagtt
     1681 ctggtacgat gatgacccca accatttgcc tgcatcagaa cgcaatccaa aaaatgtttt
     1741 aatgaaagta aacatcagcc gaaaggcctc tgctcttaca acgaatgaag aatacttatt
     1801 tgaaaatgga aagcttgtat tttatttccg gcatgaagga gaagaaggaa atgaatggga
     1861 gacccgggtt tattacaata ataaaggcag ttcttttaaa tcaagtgtaa aagcaaacgg
     1921 aaaagaactg actgcaaagg attttctgac agacgagtac agagatttca aacccaaacc
     1981 tctcagcatt atggcagaag gcagaaaact tcaggagttg tttataaaaa gcatgtaaca
     2041 aaaagcctcc gttttagcgg aggctttttg tctattgcct gtcttatctt caatttccaa
     2101 ttaccagttt tagcgtctga ctaccgctac tattacctaa ctgcaacagg tatattccgc
     2161 tcttcagtcc ttctaaattc aatgtaaaca ggtactggtt attgtttgtt ttttctctaa
     2221 ccatccttcc tcctgcatca aataactgta tccggctgta tagatcggct ccttcaatcg
     2281 taactttttg ctgggctgga ttcggatata ctttgacaat gccctgtttg tcaaaaacca
     2341 cacttacaat ctgtgataat ttagcctggt catcaaaatc cacttgtttc agccggtaat
     2401 acaccctgcc tgttttacct ggtatatccg tagtgatgta ttctttcatc atatccgaat
     2461 tgccggcacc gtttacaaag ccaatattta caaaataact gccatcaaaa cttcgctgca
     2521 cttcaaagcc tttgttatta atttctgtag cagttttcca attgagctgt acggtagtgg
     2581 cattaatttt tttggcttca aaactgagta gtgtaactgg caagccaaag ccggatgagc
     2641 ctaaaatgta ctcggtgctt tggccggtag ctacaagcac tttattagtt gtatttaaag
     2701 cagcgccggc atcaatccaa tcagtaatac tgttatcatt ccgcttgtac aatttcagtg
     2761 tgggctcatt atatccagat accaacgggt ttcctgcata aacataaatt gcattataag
     2821 tagccccggt tgtggcaccg gatacatgaa caccaaagta atgactggct ccaccggcgc
     2881 ccagtatacc aaattgataa agcggctcat ccaatacctg gtaaactgtt agccctgtaa
     2941 tcgtgccggt tgttgttggc cgggccctga agaaccaatt tgtttttgta gggtcaggat
     3001 agatgacaga ggtattcggc gtggcatcag gattcacaaa atcatgggca ctgcgatcac
     3061 caatgggtgc cccagacaaa acatattgcg gaccatttac agttgtctta aacccgctac
     3121 taacatcgta ggtcctgtta aggctggctt cgtcaaattt aaaatagaga ccaaggtttt
     3181 cttccgaatt agtgagtttt tggcacatcc ggtcccggat atcgttttgg ctgcgaacgg
     3241 ccccccaaat tctcatttca tccagaaggc catcgtacga ttcattcgta cccggcttgc
     3301 aaccaatggc atagttgcca ccggtaaaac tatatgaacc attagcggct cctttagcca
     3361 atccgtcaac ataaaaactg gttagccctg tggtattatt atatgttact gctacatggt
     3421 gccatttatt gttattgatc acactaatat cttcagaagc agcaaaagca ctaccgagtg
     3481 tgccaaaaac cattgtatta ttatcgagcc cccacataat attacctatt tgattgccgg
     3541 caaacaacca ttgattaggc tggctgctgt tgcgttttat ccatgcttca aaagttaaat
     3601 cgggcggaat accagcagca ggaatttcat ctgttatttc tacccggttt gtagaattgg
     3661 gctgaaagag caacgcatta ccgcttcccc tgctttgctt gtataaagat gtaacctggt
     3721 tagcatccag cgcagcgcca tatattttta cttcatcaat gctgccgcta aaagttgcac
     3781 ctccgcaaat tccattagca tctccaataa taatcggcgc tccgcttccg ggagtattat
     3841 aaacaacaga agcagcagag gcttttaatt ctccatctac atacagtttt tgggaaccaa
     3901 tgccatcgta ggttgccacc aggtggtgcc atttattatc attatatgct tgtgaagcac
     3961 ttatcatgct ccaacctcca ttactcaggt aaaacctggg tatgttatta ttgacagaaa
     4021 gcacataccc attcggaact ccgcaattat gctttgccac tattgcaaaa tctgccgccg
     4081 gtatattact gtaccttacc caggcactga tagcaaacgg ctgaccaaca aaatcaagag
     4141 tattggtatt ggcaagactg atgccgcttt caggatttgt aaaagaataa gcggagttgt
     4201 tattatcata tttatccgtt gttaactggc tatttccata aggtgaaccg ttccggctgt
     4261 tgctactttt gtcgtttgca tcaccattca taggcaggta ggccaacagg tcgggcataa
     4321 atggtatgtc agccagctga gccatttcat tgggagaaag ggcccgttta taaatgcgca
     4381 actcatctat tttaccatta aaaaattcca tcaaactacc agctgtgttc tgcctgcaac
     4441 ctatactcag gtagctgttg gcattcacag gtggggtaat aacagccgta cctaccgctg
     4501 ctccattgaa ataaaaggtt agggtcgtgc ctttttttat aacaccaaca tgggcccatt
     4561 gcccggtaga atacccaata atttcattgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcttg
     4741 tattaacaat atttacattt atccaggagg ctatagaaaa atcgccgccg ttgaaactcc
     4801 agtctgcatg tgcaggcgct gaaatagaac ttgtacttcc attaaaagca taagctttat
     4861 cagtttcatt ttttctgtct gtagttagca caacattact tacagtgccg tcatgcccgt
     4921 taccactttc atcattggca ttaccattaa acggataata agcaacnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 ttcccgttaa agccgccatt acaaccatta taaccgccca gcgctatatt agccgcattt
     5101 gtaatcgtat aagcaacagc gtctgtggct tttgccaccc catcaacata aataaaacga
     5161 ttcgttccat cgtaaaccgc tgttacctga tgccagttat tatctatata gctttcagtt
     5221 gtactgagag gcgtccaacc tgctccccca atccagaagg tgagtttatc attatataac
     5281 atcagaatat atccactatt ctcgccacag ttatgtttag aaacaatagg ataattatat
     5341 ccggcattgg aagttcctga aaatttaaac catgcactga tggaaaaccc tccggcatta
     5401 aaattgagcg aagatgttcc cggcacctga attttatcag aagaagttcc gttccagaca
     5461 aaactatacg ctttgtcgct ttggttaaaa cggtcagtag ttaaagaagc tccggcaact
     5521 gttccgttat aaccattacc actttcatca ttggcatttc cgttaaaagg gtaataagca
     5581 acaaggccgg ttgattcagt ctggtataac tgcaatacct gtgcatcatt cagagaaatg
     5641 ttataaatcc ggatatcatc cagcttacca ttataaatat gactgctccc acatccttca
     5701 ggctgcccga tttttacagg tatcccggtt gcaggactgt tataagctga ggcaagcgta
     5761 ccttttaaca agccgtccac ataaattttt tgatttactc catcatatgt ggccactacg
     5821 tggtgccatt tgtcatcaag aaaattttgg gaagtagtta gctgtaccca actgtttcca
     5881 ttaactgcag tccagaaact gaaagttccg ttttgtgtta caagcaggta gccgccataa
     5941 ctgccgcaaa aatgtttgcc aacaataagt tctacatttc cggagttggt gcccaacctg
     6001 gcccatgccg acaaactaaa aggtttgccg gtggagttct caacaaagtc catattagtg
     6061 gaattattca gtgtgatggc tgatccaatt ccattaaact gatatgctga gttggaaaca
     6121 ccatatctgt cgctggttag tgtggcgcca cttactgtgc cgttataccc acttccactt
     6181 tcatcattgg cattaccgtt aaatggataa taagcaacaa gaccggttga ttcattctgg
     6241 tataattgca atacctgtgc atcactcagg gctgcatcgt atatacgtat gtcatccagt
     6301 ttaccgatta aatacccaac atttccatcg atgtatggtg caagtccatt tgtatagggt
     6361 attacgccaa atgaaagttc agcactgcca cttgtagctg tatttccgct aaacgtatta
     6421 tcagtgcttt tcagaacacc attaacatat atcttttgta cacttccatt tatggtgagt
     6481 acccaatgat accattggtt aaccggtacc gatgaataag taaaggagac agcattggcg
     6541 ccacaatgcc cctgcacatt atattgtccg ctacctgata aattcaacgc cataaaaaga
     6601 gttgtgccac agctaccatt accaccataa cccagtaaac ccggcctgtt tgcaacatca
     6661 gctgtataaa accaaagaga aatagtccgg ttggtggcgg gtaacccatt agctgagtac
     6721 tcaatatgcc cactggtcat cccactgaat tcataggcat ggtttgcatt tccaaaacgg
     6781 tctgttgtta aactggcccc gacataagtg gggtttcttc catttccact ttcatcattc
     6841 gggtttccat taaaagggta ataggctacc agggtctgtg tgtaggaaac gacagagaga
     6901 gatagccagg cgaggaggag aagtgtacgc atggctgatg attttggcta tgaagttgca
     6961 ttaacaacca aatgtaaaac gatgacacct gtcaagctgc acattgatac ctgtcatgaa
     7021 aaacacttat tggctacagc catgtctttt atttaaaatt cctgaaatgg aaggtgaaac
     7081 cagtcattta ccaaattgac ggtcttttcc ttgaaggttt gatgatgata ttcattggta
     7141 tccggttcat acacatactc ttgtttccac tcattgtagt gtaatgctaa ctgttcaatg
     7201 gcgtcaagaa taaaatgtat ttcttcatct gtcatcaccg ggtgtactga cattcttatc
     7261 catcccggtt tgcaggaaag atcaccatga tggatttcat cgagtatttc gtaggaatgc
     7321 agctgatcca catgcagcag catatgacca tatgtgcctg cacaggcaca gccacccctt
     7381 gtctgtatgc caaagcgatc gtttaacagt tttaccacca ggttaaaatg tgtcccgtta
     7441 ataataaatg aaataacgcc cagccgctta tgattttgtc cttctaaaat ttctactccg
     7501 ggtatggagg atagtttttc aaaaattatt ttcaataatt cttcttcccg ttttagcata
     7561 ttttccactc ccatttcctc tttcagcttt atggccagtg cggcttttat cccttgcaga
     7621 aagggcggag ttccaccatc ttccctatgc tcaatatctg aaatgtattc atggtatttc
     7681 cagggattgg tgtacgtaac tgttccgccg cctggctgat ccggtataat gttattgtaa
     7741 agacttttat gaaaaatcaa aacaccgggt gtgccctgcc cgcctaaaaa tttatgcggc
     7801 gaaaaaaaga ttgcatcaag atggcctcct tccaacgaag gatgcatatt aatagataca
     7861 tatggggcag agcaggcaaa atcaacaaaa cataatccat tatgccggtg tacaagttgc
     7921 gccactttat gataaggagt ttggataccc gttacattcg aacacccggt gatggctgca
     7981 atcttattct ttcgttcctt atatttttcc agtagttgtt ccagcccggc aaggttaata
     8041 ttgccgttct catcgttggg aatgatctcc acatcagcaa tggtttccag ccaggatgtt
     8101 tgattggaat gatgctccat atgcgaaaca aaaatcaccg gcttcaactc cgggtcaata
     8161 tcaagatagt tgctgaaaag tgagtggatc ttcactttgc tgttggccag ctcttcatgc
     8221 gaaggcaatc gtcccggctt tacgtagtcc attattcgtt cgggaatacg caggccaagt
     8281 aaccgctgca atttgttgac agcaccggtc attccgctgc cggcaaagat cagtacatca
     8341 tccgggccgg catttacatg ctgtttgatg atatgcttgg cttgctcata agcccttgtc
     8401 atagccgtac cggtcactgt tgtttccgta tgagtattgg ctagtagcgg cataatatca
     8461 ttttgcaaac gctgttcgat cggacggtag gccctgccgc tggcagtcca gtcggcatag
     8521 accatttttt ttaatccata cggcgtctca aattctttat ctatgccaat aatattatcc
     8581 ctgtattttt taaagtactc ctgcagcatg gaggctattt tggtggccaa attacctcaa
     8641 aatcaataac agaacaggcg actaaggtca ctgtaaccca aatcaggtac gaaatagccg
     8701 gattcctgta cttttcaaat tgaacactac agaaaacagc ctgtttgttt cagtcaacaa
     8761 aaaaatcaaa caggcgtttg tttctgtaaa tttgaaactc aataataaaa gctatgtctg
     8821 ttcaggattt agaacaaatc ttccaagaaa aaattgacaa cgaagtcaag attgaaccca
     8881 aagactggat gccggatgcc tatcgcaaaa caaacctgcg gcagatcagc cagcatgcac
     8941 acagtgaaat agtaggtatg ctgcccgaag gcaattggat cagccgggct ccttccttaa
     9001 aacgtaaagc tatcttgctt gctaaagtac aggacgaagc tggtcatggc ctttatctct
     9061 attctgctgc agaaacattg gggcagtcaa gagaggaaat gatcaacgac ctgcacagtg
     9121 gtaaagcaaa atattcctcc atatttaatt atcccactct tacctgggct gatattggcg
     9181 ctattggctg gctggtagat ggcgctgcta ttatgaacca ggtcatgctg accagaactt
     9241 cttacggtcc ttatgcaaga gccatggtgc ggatttgtaa agaagaaagt tttcatcagc
     9301 gccagggctt tgaagcgctg ctggttttat caagagggtc tgctgaacaa aaagccatgt
     9361 gccaggatgc catcaaccgc tggtggtggc ctgctttgat gatgtttgga ccaagagata
     9421 ccgaaagtac caacagcgat cagagcatga aatggaagat aaaaagaaaa agcaatgatg
     9481 agctgcggca gaattttgta gatatgatcg ctgaacagat caaaacattg ggcatgaaat
     9541 tgcctgatga taaactgaaa tggaatgaag aaagaaaaca ttatgatttt ggcgagatta
     9601 actgggatga gttctggcag gtggtgaaag gcaacggacc ttgcaataaa caaagactcg
     9661 atgcccgccg caaagcctgg gaaaatggtg agtgggtgag ggaggccgca gcagcctatg
     9721 catccaagag agcagccaaa caaaaacaca tagcagccta aaacaaccaa tatgaatttc
     9781 atccgaaaat tttattacgg cgacatccct gaagaaaagg gaggtggaaa aacagatgcg
     9841 aaatcctcag gtcacgactg gcccttgtgg gaagtattta tccgtagcaa aaatggttta
     9901 gaccataaac atgcaggcag cctgcatgca gccgatgcac aaatggctat tgaaaatgca
     9961 agagatgtat atacccggcg catggaaggt gtaagcatct gggtagtgga aagcaaatac
    10021 ataacagcga gtaaccctga agaggctgga gagctgtatg aacctgcggt tgataaaatt
    10081 taccgtcacc cgacttttta caatttacct gacgaagtaa actatatgta atgagtgccc
    10141 tgattgatta tactcttcat ttggcagaca atgcactaat actaggacag cgtaacagcg
    10201 aatggtgcgg gcatggacca atactggaac aggatattgc cattaccaat atttcgcttg
    10261 acctgatcgg ccagtccagg agtttatacc agtatgccgc acaactaaaa gctgacggat
    10321 caacagaaga ttcgcttgcc tacctgagaa cagaaagaga atttaaaaac tgcttattgg
    10381 cagagcaatc caatggagac tgggcacaaa ctatgctgag acaattcctt ttcagctgct
    10441 accagtttta cctgtataaa tatttacaaa gcaatcctga taaaaccatt gctgccattg
    10501 cagaaaagtc actgaaagaa gttagctatc accttcgctg gagcagtgag tgggtaatca
    10561 ggctgggtga tggtacagaa gaaannnnnn nnnnnccgaa tgctgaccgc tattgatgaa
    10621 ctctggagat atacaggaga actttttatt gccgcacctt atgagataga attaaacatt
    10681 gatttagcct cattaaaaac agaatgggat aataaagtaa aagctgtgat ggaagaagcc
    10741 accctccctg ttcctgaaaa attgttcatg caaacaggtg gcaaaacagg tacgcataca
    10801 gaacaacttg gttatatact ctctgatctg cagtatatgc aaagagccta tcccggttgt
    10861 gaatggtaat acccaagtgc agacagaatg aaaacaacag caattgataa aaagacttta
    10921 ttcgcatacc tggaagaaat aaaagatccg gaaattcctg tattatccat tgttgacctt
    10981 ggcattgtcc gtgatatta
//
LOCUS       DAORLS010000080        10727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3307_length_10727_cov_1.857478, whole genome shotgun sequence.
ACCESSION   DAORLS010000080 DAORLS010000000
VERSION     DAORLS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3307_length_10727_cov_1.857478"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PK977_07785"
     CDS             <1..393
                     /locus_tag="PK977_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18053.1"
                     /translation="RRYMSMLPAESVYPKYDSNSYWSHYVKFLYYGSEKDKANPDIRI
                     FNKVGNAYGFLVDAAYFADFGNNIEFLLSAVIYCNSDGIFNDDNYDYDKVGLPFLKNL
                     GRVIYEYELNRKRKKNPDLRAFQFTYRE"
     gene            478..1668
                     /locus_tag="PK977_07790"
     CDS             478..1668
                     /locus_tag="PK977_07790"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRF18054.1"
                     /translation="MQLSSFLQRFNEPETLKMAKLGRELRARGIDVIDLSLGEPDFDT
                     PEHIKQAAINAINENWSHYTPVPGFLDLREAVCTKLKRDNDLDYKPENIVVSTGAKQS
                     LANAMLALVDEDDEVIIPTPFWVTYSELVKIARGKVVAIKTTPQQKFKISAEQLKQAI
                     TAKTKLFLFSSPCNPSGAVYSKDELAELAKVFIKHPGIVILSDEIYEYINFVGKHHSL
                     AEFAELKERVVIVNGLSKGFAMTGWRLGYIAAHPDIAKACEKVQGQFTSGTNSITQKA
                     AVTALTSDLRPTMEMVEEFRKRRARVLEIVKVIPGLICPEPEGAFYVFPDVSSYYGKS
                     DGETTINNAGDFSMYLLNKAHVSSVMGDAFGEPKCVRFSFANSMKNIEEGWRRIKEAL
                     NQLR"
     gene            complement(1669..2046)
                     /locus_tag="PK977_07795"
     CDS             complement(1669..2046)
                     /locus_tag="PK977_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18055.1"
                     /translation="MRFIFLLSFLLLFTACKESGKPGSSSPSIVGKWKPVEMEVPNMS
                     QDEIQNVMDNFLMEFTADGKYRGEIHTEKQAGTYTFESKTGKLTIVNNNGTDHKEEVF
                     TVKWEKDLLLLVNKEGTVKLKHQ"
     gene            complement(2058..>2561)
                     /gene="coaE"
                     /locus_tag="PK977_07800"
     CDS             complement(2058..>2561)
                     /gene="coaE"
                     /locus_tag="PK977_07800"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217297.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRF18056.1"
                     /translation="DTEAKRLMNINEKIKESIRQHFGEATYKNEDLDRKYLADIVFNN
                     PEKLELLNALIHPVTIKDAEEWMQRQAAPYSIKEAALLFESGAAENLDFVIGVYAPQA
                     LRIKRVMKRDNLAQDEIVKRINRQVNEEMKMKLCDFVITNNEQELLIPQVLKLHQHFI
                     SLANSSL"
     assembly_gap    2562..2589
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2590..2670)
                     /locus_tag="PK977_07805"
     CDS             complement(<2590..2670)
                     /locus_tag="PK977_07805"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRF18057.1"
                     /translation="MLKIGLTGGIGSGKSTVAKIFEVLGIP"
     gene            complement(2747..3139)
                     /gene="yajC"
                     /locus_tag="PK977_07810"
     CDS             complement(2747..3139)
                     /gene="yajC"
                     /locus_tag="PK977_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRF18058.1"
                     /translation="MKSKLNYIAISAVIFILSSCTPPGGEQGKGGSSGGGTIQLVFLG
                     LMILVFWLFFIRPQAKRAKNQKSFIENLGKGDKVVTIAGIHGVINKVNEDGTLNIEVS
                     PGSYLKIEKSAISMDWTAALNKPATEKK"
     gene            complement(3173..3646)
                     /locus_tag="PK977_07815"
     CDS             complement(3173..3646)
                     /locus_tag="PK977_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF18059.1"
                     /translation="MKRILVTTVLAGLLISCKSVDKKGNASSEQDTRIQDSIRYAEKQ
                     KAMNDTANYTSLTWLDSTYLDMGKVKEGQVVEVSYRFKNSGTKPLVILDVTASCGCTV
                     PEKPEQPFAPGEEGVIKAKFDSKGRPGENRKHISVDANTNPTRSHSLDFRVEVTN"
     assembly_gap    3728..3780
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3781..>4577)
                     /locus_tag="PK977_07820"
     CDS             complement(<3781..>4577)
                     /locus_tag="PK977_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HRF18060.1"
                     /translation="VTTQENEVKPGEAQKILQDHFDQTRSLLVYLTWFLTEVTRYAET
                     DAHQKASKHLPTAEDLNVNTKIAGNEVLWKILDDPALKEQFNKEKPETYTDKELIRKI
                     YLSLVETPEYKAYIAKGSREKQEEKKIIEFILNDLLLANEIFVSHIEENFSNWDDDGE
                     MVVQLLVAYLQKPGSYDFKQMLSPDKAQFAKELLQTVLDKSEYLQELIIPKLKNWDPE
                     RIALLDMIMMKMGVAEFLYFETIPPKVTINEYIDLAKDYSTQQSGQF"
     assembly_gap    4578..4613
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4725..5435)
                     /locus_tag="PK977_07825"
     CDS             complement(4725..5435)
                     /locus_tag="PK977_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18061.1"
                     /translation="MIKSGGAISSITPATPTASSVIANSAVTHGSFGNDYTVTATFNS
                     GFSGFAIGQAGYILPVSFLSFTGEKNKDVVDLKWITGFEHNNAVFEIETSKDGSNFYR
                     IGSKPSYGNASTDQHYSFTDRMPVTGMNYYRIKQIDIDSRNSYSNTIAVQFEGKNHVL
                     TVYPNPAKDRITISTPQPVHNVTIRIVSADGRIAIAETISSIQRNYELDIQSLAKGIY
                     FIEFFTLTGKNITRFVKE"
     gene            complement(5432..8332)
                     /locus_tag="PK977_07830"
     CDS             complement(5432..8332)
                     /locus_tag="PK977_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18062.1"
                     /translation="MGKTKMLKLFIFSLFIASEGIAQLSSEFTVLNASFRKTESLSEL
                     LSKTPFEARIVRDENGLLNVVKKTAVIHPYISDHNGMVDPVLQPPLNSENRIEAATAT
                     VLQNFDGIGFTAVNPADPVSATGPNHVIQMINAASGAVVKIFDKAGGTVVNSFLLSNI
                     TNYQGAGDPIVLYDQIAGRWLLNEFGYSGGVTTYINTLIFAISATDDPTGSWYVYAFA
                     DNSFFVDYQKIAIWHNAYFGTSNDFNTTGTSYFGSSIYAFERSKMLNGDPTAVAIRSR
                     LTHSTGRYFSMAPVTQEGPLPSSLSGQFAFYQNDTWTVDPADVDSLYTFEFAPNFTTP
                     ASSVISTPTQMAIAAFDDDLCAATRERCIPQQGSAIQLEALTGRIMNKVNFRKFPSYE
                     SMVLSATVDANGSGLAGIRWWELRRTSGTWSVFQEGTYAPDANHRFMGSITQDAKGNI
                     GLIYNVSGNAEHPSIRFTARNPCDPLGTMTGTELTIMDGTAANASNRYGDYNSVSIDP
                     SDNLTFWLTGMYNTATSWSTRMASFTLDDCTPSPKVRFQTSAVTVREQDANVSAGCLN
                     YKDYTVNVIIDAAPSADAILTLSTSGTATGNQDFIITPSSVTLNAANLSRPVTVRIFD
                     DGSIEGDESINISFSINNSGGNAVAADYNQTCQIKIVDNDNLPEVSKTTYAAVGFGNV
                     GAITSGPFSGSGSTDKKIQNLYLASELTSMGIAPGFITHFAWRSGSATVATFNNLNVR
                     IGFTGVSTLSGGFVSTTFSNILPTTNFTTPGTTGWMTGWTLPAPVYWNGTDNIVIETC
                     WDNTTTSSDVAVIGTTVSGYIPSALVTQNAAGSVCASAAAGTSTTRPNIRLTMSVPIN
                     TVANTLISKVASLGPNDDVPFYDASGRIMARVKNLTNFDYGCTTVEIDRAGTGTSPFW
                     NNNLSNYLTSKSFRIVPTNANPVGQYDITLYYTAAERPDMKLPLVYRGMLFK"
     assembly_gap    8460..8564
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8679..8960
                     /locus_tag="PK977_07835"
     CDS             8679..8960
                     /locus_tag="PK977_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRF18063.1"
                     /translation="MAKKVNVTPLHDRVIVKAAAAEEKTAGGIIIPDTAKENPQRGTV
                     IAAGPGKKDEPVTVKTGDTVLYGKYAGTEIQIDGEDYLIMRESDILAIV"
     gene            9056..10690
                     /gene="groL"
                     /locus_tag="PK977_07840"
     CDS             9056..10690
                     /gene="groL"
                     /locus_tag="PK977_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583723.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRF18064.1"
                     /translation="MAKQIFFDIEARNKMKKGVDTLANAVKVTLGPKGRNVVIEKKFG
                     APGVTKDGVTVAKEIELEDPIENMGAQMVKEVASKTADIAGDGTTTATVLAQSIISEG
                     LKMVAAGANPMDLKRGIDKAVSLVVESLKSQSQTVGSDSKKIQQVATISANNDETIGK
                     LIAEAFTKVGKEGVITVEEAKGTDTTVDVVEGMQFDRGYISPYFVTNSEKMEAELQNP
                     YILIYDKKISNMKDILHILEKVAQGGRPLLIIAEDLEGEALATLVVNKLRGTLKVAAV
                     KAPGFGDRRKEMLQDIAFLTKGIVISEEQGYKLENADLSYLGQATSVTIDKDNTTIVG
                     GKGKKEDITARVNQIKAQIETTTSDYDKEKLQERLAKLSGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVAYIRAIESLEAKVRGQIADEQTGMAIVRRALEE
                     PVRILTANAGIDGSIVVQKIKEGKADFGFNARTEEYEHLFKAGVIDPTKVSRVALENA
                     ASIAGMLLTTECVVADKPKKEEPHTHGAPDMGGMGY"
BASE COUNT         3034 a         2102 c         2249 g         3120 t
ORIGIN      
        1 cgccggtata tgtccatgct acccgctgaa tctgtttatc caaagtatga cagtaattcg
       61 tattggagtc actatgtaaa gtttttgtat tacgggtcag aaaaagataa agcaaacccg
      121 gacatcagaa tttttaataa agtgggcaat gcgtatggct tcctggttga tgctgcttac
      181 ttcgctgatt ttggaaacaa tattgagttt ttgctgtcgg cagttattta ttgcaatagt
      241 gacggaatat tcaatgatga caactatgat tatgacaaag ttggtcttcc tttcttgaag
      301 aacctgggaa gagttatcta tgaatatgag ttgaatcgta agcgaaaaaa aaatcctgat
      361 ctgcgtgcat ttcaattcac ttatcgtgaa taaaaattat tttgccgtat cttcaaatcc
      421 atacatttgc gactgctggc tattatccaa actcaagaaa tctcataaat gaccaacatg
      481 cagttatctt cttttttgca aagatttaat gagcccgaaa ccctgaaaat ggccaagctg
      541 ggcagggagt taagagcaag agggatagat gttattgatc ttagcctggg cgaaccagac
      601 ttcgataccc ctgaacatat caaacaagcg gctatcaatg ccattaacga aaactggagt
      661 cattatacac cggttcccgg tttcctcgat cttcgtgaag ctgtttgtac aaagctgaaa
      721 agagataatg accttgacta caagccggaa aatattgtgg tatcaaccgg agccaaacaa
      781 agccttgcta atgccatgct ggcattggtg gatgaggatg atgaagtgat tatacccaca
      841 ccattttggg ttacttatag tgaactggta aagattgccc ggggtaaagt ggtagcaatt
      901 aagacaactc cacagcaaaa atttaaaatt tcagcggaac agctgaaaca ggccattaca
      961 gccaagacaa agttgtttct tttctcttcg ccttgtaatc caagcggtgc agtttatagt
     1021 aaagacgaat tggcggagct ggccaaagtc ttcattaagc atccgggtat tgtcatcctt
     1081 tcagatgaga tttatgagta catcaatttt gttggcaaac atcacagtct cgctgaattt
     1141 gctgaactca aagaaagggt tgtgatcgtt aacggactaa gcaaaggttt tgccatgacc
     1201 ggctggaggc tgggttatat tgccgcacac cctgatatag ccaaagcctg tgaaaaagta
     1261 cagggacaat ttacaagtgg aaccaattca atcacccaaa aagctgcggt aacagccctg
     1321 acttccgatc tgcgtcccac aatggaaatg gtggaagaat ttcgtaaacg aagagcaaga
     1381 gtgctggaaa ttgtaaaggt tattcccggt ttgatttgcc cggaacctga aggtgctttc
     1441 tatgtatttc ctgatgtcag cagctactat ggaaaatcag atggtgaaac caccatcaat
     1501 aatgctggtg actttagtat gtacctgctg aataaagctc atgtaagttc agtaatgggg
     1561 gatgcattcg gagagcctaa atgtgtacgt ttttcttttg ccaacagtat gaagaatatc
     1621 gaagaagggt ggaggaggat caaagaagcg cttaaccagc ttagataact attggtgctt
     1681 cagttttaca gttccttctt tattcaccaa cagcagcaaa tctttttccc attttacagt
     1741 gaagacttct tccttgtggt cagtaccgtt attattgact atagtaagct tccctgtttt
     1801 agattcaaat gtgtatgtgc cggcttgttt ttctgtgtga atctctccgc gatacttgcc
     1861 gtcagcagta aactccatca ggaaattgtc cataacattc tgtatttcat cttgactcat
     1921 gttgggtact tccatctcca ctggtttcca tttgccgaca atactgggtg aactggatcc
     1981 tggtttgcct gattctttac aagcagtaaa taaaagcagg aaggacaaga gaaagatgaa
     2041 gcgcatagta ttttgtttta taatgaagaa ttggctaagc tgataaaatg ctggtgcagt
     2101 ttcaaaactt gcgggatcag cagttcctgt tcattgttgg taattacaaa atcacacagc
     2161 ttcattttca tttcttcatt tacctggcgg ttaattcttt ttactatttc atcctgcgca
     2221 aggttatccc ttttcatcac tcttttgata cgcaaagcct gaggtgcata aacaccaatt
     2281 acgaaatcaa gattttcggc tgctccgctt tcaaaaagca gagctgcttc tttgatagaa
     2341 tatggcgctg cctgtctttg catccattct tcagcatctt ttatggttac aggatgaatg
     2401 agggcattaa gtaattcgag tttttccggg ttgttaaaaa ctatatcggc gagatatttt
     2461 ctatccaaat cttcgttttt atatgttgct tcgccaaaat gttgccttat tgactcttta
     2521 attttttcat taatgttcat cagtcttttc gcttctgtat cnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnng ggtataccca gtacttcaaa tatttttgca accgtagact taccgcttcc
     2641 gataccacca gtcaggccga tttttaacat agagggaaat tacaaaaaac aaatcccaaa
     2701 ttccaatttt caggaatttg ggatctcgat atgtttttaa gaacttttat ttcttttcag
     2761 ttgcgggttt gttaagcgca gctgtccagt ccatgcttat agcacttttt tcaattttca
     2821 gatagctgcc cgggcttact tcaatattca aagtgccatc ttcatttact ttattgataa
     2881 caccatggat acctgcaatg gtaaccactt tatcaccttt accaaggttt tcaataaagc
     2941 tcttctggtt tttggctctt ttagcctgcg gtctgatgaa gaacagccag aatacaagaa
     3001 tcattaatcc caggaagacc aattgaatag tgcctccgcc ggaagagcca cctttaccct
     3061 gctctccacc cggaggtgta caagaagaaa gaataaaaat tacagcactg atggcgatat
     3121 aattcagttt cgatttcatt atctgatttg tttagttgtt taaattgttg tgttagttag
     3181 tgacttccac ccgaaaatcg agtgagtgtg aacgggtagg attggtattg gcatccacac
     3241 tgatatgttt cctgttttca ccggggcgtc ctttgctgtc aaactttgct ttaataactc
     3301 cttcttcccc cggtgcaaaa ggctgttccg gtttttcagg tacggtacat ccgcaactgg
     3361 cagttacatc taaaatcaca agcggcttgg tgccgctgtt cttaaagcgg taagatacct
     3421 ctacgacctg tccttctttt actttaccca tatctaaata ggttgaatcg agccaggtga
     3481 gtgaagtata attagccgta tcgttcattg ccttttgttt ttcggcatag cgtatactgt
     3541 cctgaatgcg ggtatcctgt tcggatgaag catttccttt cttatcaacc gacttacagg
     3601 aaataagcag gccagcaaga actgttgtta ctaatattcg tttcatctgc ttagaaattt
     3661 aggagttgca aaagtaacgg taaagaggcg ggtgaacaag ccattaattt tttttataat
     3721 ctactttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 caaactggcc actttgctgg gtactgtaat ctttggccag gtcaatatat tcgttaatag
     3841 ttactttagg aggaatcgtt tcgaagtaca gaaattctgc tacacccatt ttcatcatga
     3901 tcatatccag cagggcaatt cgttccgggt cccagttttt cagttttggg attatcagtt
     3961 cctgcaggta ttcgctttta tccaaaacag tttgcagcaa ctctttggca aattgagctt
     4021 tatcgggact taacatttgt ttgaaatcgt aactgccggg tttctgtaag taagcaacca
     4081 gtaactgcac taccatttct ccgtcatcat cccagtttga aaaattttct tcaatgtgcg
     4141 atacaaatat ttcattggcc aacagaaggt cgttcaggat gaattcaata atcttttttt
     4201 cttcctgttt ttcccttgaa cctttggcga tgtaggcttt atattctggt gtttccacca
     4261 ggctgagata tatcttcctt atcagctctt tatccgtgta agtttcaggc ttttctttat
     4321 tgaactgttc ctttaacgcc ggatcgtcca gtattttcca gagcacttca ttgcctgcta
     4381 ttttggtatt tacattcaaa tcttcggcgg taggcaggtg tttggaagcc ttttgatggg
     4441 catcagtttc tgcataacgg gttacttctg ttaaaaacca ggtgagatag accagtaaag
     4501 aacgggtctg gtcaaaatga tcctgtaata tcttttgcgc ttcgccgggt ttaacctcat
     4561 tctcctgggt ggttactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctgctg
     4621 atcatgcgtt aagaactaat tgaagggcga agataggcga ttttgttttg caactaaaat
     4681 ggggtagttg ttaagctacc cccattttat atataagaat ctatttactc ttttacaaac
     4741 cgggtgatat tttttccggt taaagtaaaa aattctataa agtaaattcc ttttgcaaga
     4801 gattgaatat ctagttcata attgcgttga atgcttgaaa tcgtttcagc aatggcaatt
     4861 cttccgtcag cactaacaat tcgtattgtt acattatgaa ctggttgagg tgtacttatc
     4921 gttatgcggt ctttcgccgg attaggataa actgtcaata catggttttt tccctcaaat
     4981 tgtacagcaa ttgtattgct gtagctgtta cggctgtcaa tatcaatttg cttaatcctg
     5041 taatagttca ttccggttac aggcatacgg tctgtaaagg aataatgctg gtcagtactt
     5101 gcatttccat aactgggttt actgccaatc cggtagaaat tactgccatc tttagatgtt
     5161 tcaatttcaa atactgcatt attatgctcg aaacctgtga tccattttaa atcaacaaca
     5221 tccttgtttt tttctcctgt aaatgaaagg aaagatacag gtaaaatata gccagcctgc
     5281 ccgattgcaa aaccggaaaa tcctgagttg aaagttgctg ttacagtata atcattgcca
     5341 aaactgccgt gtgtcactgc gctattggct attacggaag atgcagttgg cgttgctggg
     5401 gttatgctgc taatagctcc tccagacttg atcatttgaa caacattcca cgataaacca
     5461 gtggcagttt catatccggc ctttcagctg cagtataata caacgttata tcatattgcc
     5521 cgacggggtt tgcgtttgtt ggtacaattc tgaacgattt tgaagtcagg tagtttgaca
     5581 ggttattatt ccagaatgga gaagtgccag ttccagcacg gtcaatttct accgttgtac
     5641 aaccataatc aaaattggtc agatttttaa ctcttgccat aatcctcccg ctggcatcat
     5701 aaaatggcac atcatcattg gggccaagac tggctacttt tgaaataagt gtatttgcta
     5761 cggtattaat aggtactgac atggtgagcc ggatattagg ccttgttgta cttgtacctg
     5821 ctgcggctga agcacataca gaaccggcag cattctgtgt tactaaagct gatggaatgt
     5881 agccacttac cgttgtgcca ataactgcaa catctgaaga ggtggtagta ttgtcccaac
     5941 aagtttcaat aacgatatta tctgtaccat tccagtagac tggagctggt aatgtccagc
     6001 ctgtcatcca gcccgttgtg cccggagtag tgaaattagt agtcggtaaa atgttactaa
     6061 atgtagtgct gacaaatcca cctgaaagcg tagaaacacc agtgaaacca atacggacat
     6121 tcaaattgtt gaatgtggca acggtagcag agccagacct ccaggcaaaa tgtgtaatga
     6181 acccgggtgc aatacccatg ctggtaagtt cagatgccag gtaaagattc tgaatttttt
     6241 tatcagtact tccactgcca ctgaatggac ctgaagtaat agcgccaaca ttgccaaagc
     6301 ccacagcagc gtatgtcgtt ttggatactt ctggcaaatt atcgttatca acgattttta
     6361 tctgacaggt ttggttatag tcagctgcaa cagcattgcc tccactatta ttgatggaaa
     6421 aactaatatt aatactctca tctccttcaa tggaaccatc gtcgaatatt cttactgtaa
     6481 caggcctgct gaggttggct gcattaagcg taacggatga aggtgttatg ataaaatcct
     6541 gattgccggt tgcagtacca ctggtactta aagtaagaat ggcatctgca cttggggcag
     6601 catcgattat tacatttacg gtataatctt tatagttaag acatcctgca ctcacattag
     6661 cgtcttgttc cctgacagtg acagcagaag tttgaaaacg tactttaggt gaaggggtac
     6721 aatcgtccaa tgtaaaagaa gccatacggg tactccagct tgtagcagtg ttatacatgc
     6781 cagtcaacca aaaagtaaga ttgtcggaag gatcaatact gacagaatta taatcaccat
     6841 atcggttgct ggcattggct gctgttccat ccataatagt tagttcagtt ccggtcattg
     6901 ttcctaaagg atcacaagga ttacgggcag taaatcttat agatggatgt tcagcatttc
     6961 cactcacatt gtaaataaga cctatattac cttttgcatc ttgtgtgatg gagcccataa
     7021 agcggtgatt ggcatcaggg gcataagttc cttcctggaa aacactccag gtaccactgg
     7081 ttcgtcgaag ttcccaccag cgtataccag ctaagccact accattagca tctacagttg
     7141 cagatagcac catagactca taggatggaa attttctgaa gtttactttg ttcataattc
     7201 tacctgttaa tgcctcaagc tgaattgccg agccttgttg tggaatacac ctttccctgg
     7261 ttgcagcaca cagatcatca tcaaatgctg ctatggccat ttgagtaggg gtgctaatga
     7321 cagaacttgc cggtgtagtg aaatttggag caaattcaaa agtgtaaagg ctgtccacat
     7381 ctgcaggatc gacagtccag gtgtcatttt ggtaaaatgc aaattgcccg ctaagagatg
     7441 atggcaaagg cccctcttgg gttacaggtg ccatactaaa gtaacggccg gttgaatgtg
     7501 tcagccttga tctgattgcc acagcagttg ggtcgccatt tagcatcttg ctacgttcaa
     7561 aagcatatat agaagagccg aaataggaag tacctgttgt attaaaatca ttggatgttc
     7621 caaaataagc attgtgccaa atggcaattt tttggtagtc tacaaaaaaa gaattgtcgg
     7681 caaatgcata aacataccag gacccggttg ggtcatcggt agctgaaatg gcaaatatga
     7741 gagtattaat ataagtagtg acacctccgg aataaccaaa ttcattcagg agccatctgc
     7801 cggcgatttg atcatacaaa acaatcggat cgcctgctcc ctggtaattg gttatattac
     7861 taagcagaaa gctattcaca actgtacccc ccgccttatc aaaaatttta acaaccgctc
     7921 cggatgctgc atttatcatc tggattacat ggttgggtcc ggttgcactt accggatcag
     7981 caggattaac agctgtaaaa cctatcccgt caaaattttg aagaactgtt gcagtggcgg
     8041 cttcaatcct gttttcactg ttcaaaggcg gttgtaaaac cggatcaacc attccattat
     8101 ggtcagatat atatgggtgt ataacggcag tctttttaac tacgttcaat aatccgtttt
     8161 catccctgac tatccttgcc tcaaatggtg tttttgaaag tagctcactc aacgattcag
     8221 tttttcggaa actagcattt agtaccgtaa actctgaaga taattgggca attccctcag
     8281 aggcaataaa aagggaaaag atgaaaagtt taagcatttt agttttaccc atgagtagta
     8341 agtatttata cttaaaaata cgtttccatg ttttgtgggt ccaccattat tatccacagg
     8401 tgggaattaa tcattttgat tatttccggg agtcactttt tctgactttg cgccgggtan
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatttc cggttataaa
     8581 gccccggcat agtatttcct gtaccttcgc cgccctgcct ccaaagaaat aaagcgggca
     8641 ggtagtttca attcaaaaaa ctcaaaatca atacaattat ggctaagaaa gtcaatgtta
     8701 ccccccttca tgacagggta attgtaaaag ctgcagcagc ggaagagaaa actgccggtg
     8761 gtattattat ccccgataca gctaaagaaa accctcagcg tggcaccgta atcgccgcag
     8821 gacccggcaa aaaggatgaa ccagttaccg ttaaaacagg cgacactgta ctgtatggca
     8881 agtatgcagg aacagaaatc cagatcgatg gcgaagacta tctcatcatg agagagtcag
     8941 acattcttgc aattgtataa tgtgaaaaaa aagaatctgc cggattgaaa atggcattta
     9001 tcatttcaac attaattgcg aattatctta ttaattaaat atcaaattaa atattatggc
     9061 aaaacaaatc ttcttcgaca ttgaagcaag aaataaaatg aagaaaggcg ttgacaccct
     9121 ggccaacgct gtgaaagtaa cccttggtcc taaaggccgt aatgtggtta ttgagaaaaa
     9181 attcggtgca cccggcgtta ctaaagatgg tgtaaccgtt gctaaggaaa ttgagctgga
     9241 agatccaata gagaatatgg gtgcccagat ggtaaaagaa gtagcttcca aaactgctga
     9301 tattgcgggt gatggtacca caactgctac tgtattggcc cagtctatca tcagtgaagg
     9361 attgaaaatg gtggctgccg gtgctaatcc tatggacttg aaaagaggta ttgacaaagc
     9421 cgttagtctt gttgtggaaa gcctgaaatc tcagagccag accgtaggaa gcgacagcaa
     9481 aaaaatccag caggtagcaa ctatctctgc caataatgat gaaacaatag gtaaactgat
     9541 tgctgaagcc ttcaccaaag ttggtaaaga aggtgtgatc accgtagaag aagcaaaagg
     9601 tactgacact actgtagatg tggtagaagg tatgcagttt gaccgtggtt atatctctcc
     9661 ttatttcgtt accaacagcg aaaaaatgga agctgagctg caaaatcctt acatcctgat
     9721 atatgacaaa aagatcagca atatgaaaga cattctccat atcctggaga aagtggcaca
     9781 aggcggtcgt cctttgctta ttattgccga agatctggaa ggtgaagcac tggctacact
     9841 ggtggtgaac aagctccgcg gcacattgaa agtggctgct gttaaagctc cgggctttgg
     9901 cgatcgcaga aaagaaatgc tgcaggatat cgcttttctt acaaagggta ttgtgatcag
     9961 cgaagagcag ggttacaaat tggagaatgc cgaccttagc tatcttggac aggctacttc
    10021 agtaacaatt gataaagaca acaccactat cgtgggtggc aagggcaaga aagaagatat
    10081 tactgcccgt gtaaaccaga ttaaggccca gatcgaaacc actacttccg attacgataa
    10141 ggaaaaatta caggaacgcc tggcaaaact gagcggtggt gtggcagtac tttatgtggg
    10201 tgcggctacc gaagtggaaa tgaaagaaaa gaaagatcgt gtagatgatg ctctgcatgc
    10261 cactagagct gctgtggaag aaggtatcgt tccgggtggt ggtgtggcct acatccgtgc
    10321 tattgaaagt cttgaagcaa aagttcgtgg tcaaatagcc gatgagcaaa ctggtatggc
    10381 cattgttcgc cgtgcattgg aagagcctgt tcgtatcctg actgctaatg caggtattga
    10441 cggttccata gtggtgcaga aaatcaaaga aggcaaggct gactttggtt tcaatgcaag
    10501 aactgaagaa tatgagcacc ttttcaaagc aggtgttatt gacccgacta aagtgagccg
    10561 tgtagcattg gaaaatgctg cttcaattgc aggtatgtta cttaccactg aatgtgtggt
    10621 agctgacaaa cctaagaaag aagagccaca tacgcatggt gctcctgata tgggtggcat
    10681 gggctactaa aaataccttt gtcaaaaaag ctgaaaggcc cctgtca
//
LOCUS       DAORLS010000081        10721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3310_length_10721_cov_2.340240, whole genome shotgun sequence.
ACCESSION   DAORLS010000081 DAORLS010000000
VERSION     DAORLS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_10721_cov_2.340240"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK977_07845"
     CDS             <1..264
                     /locus_tag="PK977_07845"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRF18065.1"
                     /translation="AFAVFCAVMLFLDKNDLFTQMERQKELRDLQQSKKHYTTQIATE
                     RKELEALKNNPATLEKYAREKYLMKRDNEELFIISEKPDNTNN"
     gene            370..603
                     /locus_tag="PK977_07850"
     CDS             370..603
                     /locus_tag="PK977_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18066.1"
                     /translation="MTIFTPEDLLLYLYKETSDDQTKAIEEALQKDWTLREKLTVLKA
                     SMQRLDKIVVSPRTEVVLNVLNYAREKSAEEVQ"
     gene            710..1351
                     /gene="nth"
                     /locus_tag="PK977_07855"
     CDS             710..1351
                     /gene="nth"
                     /locus_tag="PK977_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRF18067.1"
                     /translation="MTRKERYTFVIDYFEKNMPVAETELEFSDPYQLLVAVILSAQCT
                     DKRVNMTTPAIFEKYPTVQSLSKATFEDLLPLVKSISYPNNKTKHLIGMANKVVNQFH
                     GAIPMTVEELVQLPGVGRKTANVITSVVDKQPNMAVDTHVFRVSKRIGLVPQTASTPL
                     AVEKILIKNIPGHLIHKAHHWLILHGRYTCLARTPKCEKCGLQTVCRYFSTLQ"
     gene            1450..2367
                     /locus_tag="PK977_07860"
     CDS             1450..2367
                     /locus_tag="PK977_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18068.1"
                     /translation="MRKYFVLAILSSVFYSTTLKAQYYYYNNKYYDNAVVMELGVSGG
                     IMNALTDLGGKKGVGKNFIKDLRWATARPSYGFYATAMYKNMAGLRLEGTFGEVTGYD
                     SILKNVAGSTFGRYERNLSFKSKISEIQLAFEVHPLFFKDYDEDEAPRISPYAIVGGG
                     IYTFDPQAELNGQWYALQPLRLEGQGFNEYKDRKTYELTQFNLLGGLGLKYEINSFLN
                     ARLEFVHRKLFTDYLDDVSTEYVDPTLFTNYLPANLAAVAQQLYSRRQELVPSDVPVA
                     GEQRGDPKDDDAFFTIQLKIGVTLGRLRR"
     gene            2510..>3709
                     /locus_tag="PK977_07865"
     CDS             2510..>3709
                     /locus_tag="PK977_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18069.1"
                     /translation="MQTDKQPPAEVKFNSNLCFRPLVMYLKKNIAEGNPGMLKLYGQV
                     VEEFESHPELLEPITDINDLRSHSELIEELLSAVFPPTTSNFMYGVSIPFKPEAVYVS
                     PLFKKQLLKPKTNIITIPEFDLNTALTPQRLQFAYGLILKKYYGLESSQTYRSVYSNP
                     DADTGLVRYMELRVDGRFIEVRPVGELPKLPESVIESQTNRLMNITEMMQHIPLEKFV
                     FDGLFVLRINDMTEQEVIAKMKNRFLDNNAFSDSSIYNELEEQVQCLIGMKDVSIGIT
                     PFFKINNHYVYSDLHNTNSILFRHFKSIPDKDEISDYCKLLFRDNNQPLLFETLTEQS
                     INEVQCLHYYHLEGGRSLILCPLKLKKDLIGMLEITSTIPGQLKPQHISKIEPAIPLF
                     TLGLEKSL"
     assembly_gap    3710..3719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3720..>4695
                     /locus_tag="PK977_07870"
     CDS             <3720..>4695
                     /locus_tag="PK977_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRF18070.1"
                     /translation="EQLNSQVDRVIKKKFTAVQPAVEWKFTEAALNYIVNLSTKEDVK
                     IERIAFDEVFPLYSAIDIRNSSTERSHSIQLDIVEQLELALRVVKKAQAEISFPLLQE
                     IEFKINKYIIAASDVLQSEEEMSINEFFHGQITSVFNHLRSVEPSLKNVIEEYFSALD
                     PQVGMLYKHRKDYENSVTRINETLSRFIDKEQIAAQKVYPHYFERYVTDGLEFNIYMG
                     QSLTPRKKFDEIYLRNLKMWQLTVLAKASRLTHNLESELTIPLRTTQLILSHNQALAI
                     LFRTEERKFDVDGAYNVRYEIIKKRIDKVRIKETNERLTQPGKIAIVYT"
     assembly_gap    4696..4705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4706..4912
                     /locus_tag="PK977_07875"
     CDS             <4706..4912
                     /locus_tag="PK977_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18071.1"
                     /translation="DAEEYTEYIEFLQNKNLLKPGIEKHDLEELQGVSGLKALRVDVN
                     FDDLAPAQKKVELSATTSQELIGK"
     gene            complement(4914..6074)
                     /locus_tag="PK977_07880"
     CDS             complement(4914..6074)
                     /locus_tag="PK977_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF18072.1"
                     /translation="MKRRLVVMIVLATAIASCGKKSTGKAGFSVSGTITNSPVKMVYL
                     EEIPMTTMQPMLVDSSAVDAKGKYSLKAKATEASVYNIRLEGSAYPLAGVINDADKIT
                     VDATFNKENNQFAEKYEIKGSPASQQMKDFMAGVNDKLQFIFTNDKLLDSLEKGGAKD
                     SLLTSVRNQSLQAAFDIKALLDTTVSNSKNPALTMIVLGYYQSMSGNPAFRLEPLSKD
                     DVREIVNNTAEKFPDHKGIAAIKQSLEGWIGKPAPEISLPDPDGKEVKLSSYKGKFVL
                     VDFWASWCGPCRAENPNVVNAYTKFKDKNFDILGVSLDRPGEKDKWLKAVKDDKLTWT
                     HVSDLQYWNSAVVPLYKIEGIPYNVLVDPSGKIIAENLRGESLDKKLSELLK"
     gene            complement(6094..7554)
                     /gene="gatB"
                     /locus_tag="PK977_07885"
     CDS             complement(6094..7554)
                     /gene="gatB"
                     /locus_tag="PK977_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRF18073.1"
                     /translation="MPTCLMTDYSAYEIVVGLEIHAQLLTQSKLFCGDSIAFGAEPNT
                     HVSPITLGHPGTLPKMNRKAIEYAIKMGLACNCTIQQYNYFARKNYFYPDLPKGYQVS
                     QHTIPICTGGFVKIKTNSGEKQVRLNRIHLEEDAGKSLHDIDPDNTCVDYNRAGTPLI
                     EIVSEPDLRSSDEVFAYVTEVRKLVRYLEICDGNMEEGSLRCDANISVRKKGDTKLGT
                     RVEVKNLNSIRNVKRAVDAEAKRLIDMLEKGGIINQQTRSFDANTGTTFAIRDKEDAD
                     DYRYFAEPDLTPFQLQDEFIDRIRKSLPALAEQRIQQYISSYQLSEYDAAVLTEEKAF
                     ADYFESIIKHTNNFKAAANWMLGPVKTWMNEEVKEIGSFPLQPLKLAELIGLIDSGKV
                     SFSAAAGKLFQHLLKEPLADPGQLASQLNLLQESDASTIEPIVEKVLEKLADKVADYK
                     KGKKGLLALFVGEVMKQSKGKADPKLTNQLLLEKLK"
     gene            complement(7660..8700)
                     /locus_tag="PK977_07890"
     CDS             complement(7660..8700)
                     /locus_tag="PK977_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF18074.1"
                     /translation="MRKISVKNAVLAAAIALFAPATLLAQKDEKDKTVKEERKDVQQI
                     IITKKSGADDKVVVEINGDKITINGKPFEDYKDKGGDIKVKLNRLKDVEALTLERAPR
                     GGVWNFNDNNDNFRFYSIDENRAMLGVTTQKVTEGAEVQSITPESAAEKAGLKENDII
                     TKVDDKKIASPDDLSEAIKAHKPGDKVTITYLREGKEKKATVELTKWKGAKNWSMGDN
                     FKIDLGDMDFESIMPKIKEIPIPKAPFGQNWTWTGGGPKLGLSVQDTDDGKGVKVIEV
                     DDESNAEKAGIKNNDIITEVDGKAINNADDMVRVVKESKDKVSVMIKLKRDGKTQNVE
                     VKMPRKLKTADL"
     gene            complement(8815..9333)
                     /locus_tag="PK977_07895"
     CDS             complement(8815..9333)
                     /locus_tag="PK977_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF18075.1"
                     /translation="MNIVIETERLILRTFTEDDGMLIYELNKDPDVTKYTGDPVRDIE
                     HACQILEQVILPQYALYNHGRWAVHVKSGSEFIGWCGLKARPERNEIDLGYRFMKSAW
                     GKGFATEAAFACLQYGFEKLNLPRIVGRAMPQNTPSLKVLEKCGMKYIGEEIVDDHPA
                     VTYEAINPFIRS"
     gene            9430..9582
                     /locus_tag="PK977_07900"
     CDS             9430..9582
                     /locus_tag="PK977_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18076.1"
                     /translation="MVYIILVLFAIVCIFLTIYAIRIYKEDSHIRAERKRILEEEEED
                     LFGGKL"
     gene            complement(9577..>10721)
                     /locus_tag="PK977_07905"
     CDS             complement(9577..>10721)
                     /locus_tag="PK977_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRF18077.1"
                     /translation="LDTTPFYAESGGQVGDKGWLQIGSETVEVTDTKKDNDLIIHFTE
                     RFPEEVSGEIIAKVDAVKRKNTALHHSATHLLHAALRKVLGTHVAQKGSLVNEKHLRF
                     DFSHFAKVTDEEIVKIEAIVNEKIRENIPVVIKSLPKDEALKLGAMALFGEKYGDVVR
                     VVIIDPSYSVELCGGTHVGSTGELGLFKLTSESAVAAGVRRVEAVCGKQAEEYVNENL
                     LLLDKLKEALKNPKDIQKAIENLQVENAELKKHLEAMEARQLVAIRNELLQKDEIING
                     VNFVGDIIEVSSADALKKLCYDLKNHLRDHVAVLCVNLGGKPFVAVGISETVVTAKNL
                     DAGKIIKETIAPLIKGGGGGQKTLATAGGQDAGKLGEVIEAVKKLL"
BASE COUNT         3029 a         2407 c         2027 g         3238 t
ORIGIN      
        1 gcttttgcag ttttttgcgc tgtaatgctg tttttagata aaaacgacct tttcacccaa
       61 atggaaaggc aaaaagagct tcgtgacctg cagcaaagca agaaacatta caccacccaa
      121 attgctacag aacgtaaaga attagaggca cttaagaata accccgccac cctggaaaaa
      181 tatgcccggg aaaaatacct gatgaaaagg gataacgagg aacttttcat tatatcggaa
      241 aaacccgata ataccaataa ttagcattct ccacacaata aggcaaacca gcccccgtta
      301 ataagacgga cgttcacaaa atcagttatc aaggacaaat taatttttgg acatttaatc
      361 ggtttgccaa tgaccatttt tactcctgag gatcttttat tgtatttgta caaggaaacc
      421 tccgacgatc agacgaaggc tattgaagag gctttacaaa aagactggac acttcgtgaa
      481 aagctgaccg ttcttaaagc ttcgatgcaa agacttgaca aaatcgttgt ttcgccccgc
      541 acagaagtag ttcttaatgt actgaactat gcaagggaaa aatcagctga ggaagtacaa
      601 taaccaatta accaacgtaa aagcatttaa tcctggccgc aagccgggat ttttttattc
      661 tatacctccg cttgtttgcc ggttattttc tattcgcttt atttgcagca tgacccggaa
      721 agaacgttac actttcgtaa ttgattattt tgagaagaat atgcccgttg ctgaaaccga
      781 attggaattc agtgatccgt accagttatt agtggcagta attctttctg cccaatgtac
      841 cgataagagg gtgaatatga ccacccccgc tatattcgaa aaatacccaa ctgttcaatc
      901 gcttagcaaa gcaacatttg aggacttgct ccccctggta aaaagtattt cctatcccaa
      961 caacaaaaca aagcacctta ttggaatggc caataaggta gtgaatcaat ttcacggagc
     1021 cataccgatg acagtggaag aactggtgca attaccggga gtaggaagaa aaaccgccaa
     1081 tgttattaca tcggtggtag acaagcagcc taatatggcc gttgataccc atgtgttcag
     1141 ggttagcaaa cggattggtt tagtgccaca gacagcctct actccattag ctgttgaaaa
     1201 gatactgatc aaaaatatcc ctggccacct tattcataaa gcccaccact ggctgatctt
     1261 acacggccga tatacctgcc tggcaagaac ccccaaatgt gagaaatgcg ggttacaaac
     1321 agtttgcagg tatttttcaa cattacaata aattaaactt caccggtatc gtttcttcct
     1381 atttttattt ttcctctcat caatccactc cctttgtaaa atcaacgttt aacctgaaaa
     1441 agattgagga tgagaaaata tttcgtactg gcgattttaa gcagtgtttt ttatagtaca
     1501 accctgaaag ctcagtatta ttattacaat aataaatatt acgataatgc tgtagtgatg
     1561 gagttgggcg tttctggtgg tattatgaat gcactaacag atttaggcgg caaaaaaggt
     1621 gtaggaaaaa actttataaa agaccttcgt tgggctactg ccaggccaag ctatggtttc
     1681 tatgcaacag ccatgtataa aaacatggct ggcctccggc tggaagggac gtttggcgaa
     1741 gtaaccggtt atgacagcat cttgaaaaat gttgcgggtt caacttttgg ccgctacgaa
     1801 cggaacctga gtttcaaaag caagatcagc gaaatacaac tggcctttga agtgcaccct
     1861 ttgttcttca aggattatga tgaggatgaa gcgccacgca tttcgcctta tgcaattgtt
     1921 ggtggtggta tttatacatt cgacccgcag gcagaactta acggacaatg gtatgccctt
     1981 caaccattaa gactggaagg acaaggattt aatgagtaca aggacagaaa gacttatgag
     2041 ttgacccagt tcaatctgct gggcggttta ggcctgaaat acgagatcaa ttcatttctg
     2101 aatgcccggc ttgaatttgt gcacagaaaa ctctttaccg attatcttga tgacgtaagt
     2161 accgaatatg tagacccaac tctttttacc aattatttgc ctgcaaacct cgctgctgtt
     2221 gcgcaacagt tatacagccg cagacaagaa ctggtgccga gcgatgttcc tgttgccgga
     2281 gaacaacgtg gcgatccaaa ggacgatgac gccttcttca ctattcagct taagatcggc
     2341 gtaactcttg gcagactaag aagatagtac caaaagcttc ggtgaaaacc ggtaatcagc
     2401 ggttttttta tggtcttggt gattgtttat ttcaaaagag tcactgcgaa acaaataaca
     2461 tatttgttaa ttttaccgga gccccgcaac agtaattaaa acttctgcta tgcaaaccga
     2521 taagcaaccc cctgctgaag taaaattcaa cagcaatctt tgtttcaggc ccctggtaat
     2581 gtatctgaag aagaatattg ctgaaggtaa ccccggcatg ctgaaacttt atggccaggt
     2641 ggtagaagag tttgaatctc atcccgaatt actggaaccc attactgata tcaacgacct
     2701 tcgttctcac agtgaactga tcgaagaatt attatcagcc gtctttcccc ctaccacctc
     2761 caattttatg tacggcgttt ccatcccttt taaaccggaa gccgtttatg tatcgccgct
     2821 gtttaaaaaa caactgttga aaccaaagac aaatatcatt actatacctg agtttgatct
     2881 aaatacagcc cttaccccac aacggttaca atttgcttat ggacttatct tgaaaaaata
     2941 ctatggactt gaaagttcac aaacatacag atctgtttat tctaacccgg atgcagatac
     3001 aggcctggtt cgttatatgg aattgcgggt agatggtcgt tttattgagg taagacccgt
     3061 tggtgaactg cccaaactac ctgaaagtgt tattgaatca cagaccaacc ggttgatgaa
     3121 tattactgag atgatgcagc atattccgct ggagaaattc gtatttgatg gactttttgt
     3181 tttacgcatc aatgacatga cagaacagga agtgattgct aaaatgaaaa accgcttcct
     3241 tgacaataat gcgttttcgg attcttctat ttacaacgag ttggaagaac aggtacagtg
     3301 cctcataggc atgaaggatg tttcaattgg cattactcct tttttcaaaa tcaataatca
     3361 ctacgtatac tctgacttac acaatacaaa tagtattctg ttcaggcatt tcaaatccat
     3421 acctgataaa gatgaaataa gtgattattg caaactgctc ttcagggata ataaccagcc
     3481 tcttctcttc gaaacactga cagaacaatc catcaatgaa gtacagtgcc tgcactacta
     3541 tcacctggaa ggtggccgga gccttattct ttgcccactg aaattaaaaa aagacctgat
     3601 tggcatgctg gagatcactt ctacaatacc cgggcaattg aaacctcagc atatcagtaa
     3661 aattgagccg gccataccat tatttacgct tggattagag aaaagccttn nnnnnnnnng
     3721 aacagctgaa ctcacaggtt gacagggtta tcaaaaagaa atttaccgcc gtgcagccag
     3781 ccgtagaatg gaagtttact gaagcagctc ttaattatat agttaacctg tctacaaaag
     3841 aagatgtaaa aattgaacgc attgcctttg atgaggtgtt cccgctgtac agcgcaattg
     3901 atatccgtaa ttcatctacc gaaagatcac attctatcca actcgatatc gttgaacaac
     3961 tggaactggc attaagagtt gtaaagaaag cacaggctga gatatcattc cccttattgc
     4021 aggaaataga atttaagatc aacaagtata ttatcgcggc ttcagatgtg ttgcagtcag
     4081 aagaagaaat gagcatcaat gagttttttc atggccagat aacctctgtt ttcaatcatt
     4141 taagatcggt ggagccatca ttgaaaaatg taattgagga gtatttcagt gctctcgacc
     4201 cccaggtggg catgctatac aaacacagaa aagactatga gaacagtgtg acccgtatca
     4261 acgaaacatt atcgagattt attgacaaag aacaaatagc tgcgcaaaaa gtatatccgc
     4321 attattttga acgttatgtg accgatgggc tggaattcaa tatttacatg ggccagtcac
     4381 ttacgccgag gaagaagttt gatgaaattt atttacggaa tctgaaaatg tggcaactga
     4441 ccgtactggc aaaagcctca cgacttactc acaacctgga aagtgaattg acgattcctc
     4501 ttcgtacaac acagcttatt ctttcacaca accaggcgct ggcaatactg ttccgtactg
     4561 aagaaagaaa atttgatgtg gacggagcct acaatgttcg ttacgagatc ataaagaagc
     4621 gtattgacaa ggtaaggatc aaagaaacaa acgaaagact tacccagccc ggaaaaattg
     4681 cgatcgtata tacacnnnnn nnnnngatgc agaagagtat actgagtaca ttgagtttct
     4741 tcaaaacaaa aatctgctca agcctggtat cgaaaagcat gacctggaag aactgcaggg
     4801 tgtttcagga ttgaaggcct tgcgggtgga tgtaaacttt gatgatctgg caccggcaca
     4861 aaaaaaggtt gagctatccg ctacaacctc ccaggaactt ataggtaagt aaattatttc
     4921 agcaactcac taagcttttt atccagcgat tctccccgca ggttttcagc aatgattttt
     4981 cctgaaggat caaccagtac attatagggg ataccttcaa tcttgtacaa tgggacaacg
     5041 gcgctgttcc agtattgcaa gtcgctaaca tgagtccagg tgagtttgtc atctttaacg
     5101 gctttcagcc atttatcctt ttcacccggc ctgtcaagtg aaacgcccaa tatatcaaaa
     5161 ttcttatcct tgaatttagt atacgcattt accacgttgg gattttctgc acggcagggt
     5221 ccgcaccaac tggcccagaa gtcaaccaat acaaattttc ctttgtaaga actgagtttc
     5281 acttcttttc catctggatc aggcaggctg atttcaggtg ccggctttcc aatccatcct
     5341 tctaatgact gtttaatggc agctattcct ttgtgatcgg gaaatttttc agcagtattg
     5401 tttacgattt ctcttacatc atccttagag agcggctcca gcctgaatgc gggattacct
     5461 gacatgctct ggtaatatcc caggacaatc atggttaaag ccgggttttt tgaattacta
     5521 acagtagtgt ccagcagggc tttgatatca aaagcagcct gcaaggactg attcctgaca
     5581 gaggttagca gactgtcttt tgctccacct ttttccaaac tatccagcaa cttatcattt
     5641 gtaaaaatga actgaagttt atcatttact cccgccataa aatccttcat ctgctggctg
     5701 gcaggcgacc ctttgatttc atatttctcg gcaaactggt tattctcttt gttgaatgta
     5761 gcatcaactg ttattttatc agcatcatta ataactcctg ccagcggata ggcgctccct
     5821 tccaggcgaa tattatacac actggcttca gttgcctttg ctttcaatga atattttcct
     5881 ttggcatcta ccgcagagga atcaaccagc atgggctgca tggtcgtcat gggaatttcc
     5941 tccaggtaaa ccattttcac agggctattg gtgatagtac cactgacact gaaaccggct
     6001 tttcctgttg attttttacc acaggatgca atggcagttg ccagcactat catgactact
     6061 aatcgacgtt tcatttttaa agattaagat tgtttatttt aatttttcca gtaataattg
     6121 gttagtaagt tttggatctg ccttgccttt tgattgtttc attacttcgc ctacaaataa
     6181 cgccaaaagc cctttcttcc cctttttata atctgccact ttatcggcca gtttttccag
     6241 caccttttct actatcggct caatcgttga tgcatcagac tcctgcaaaa gattcaactg
     6301 gctggctaat tggccaggat ccgccagagg ttctttaagc aggtgctgaa atagtttacc
     6361 cgcagcagcc gaaaagctca ctttaccgct gtcaattaat ccaatcagct cggctaattt
     6421 caaaggttgc aaaggaaaac tgccaatctc tttaacttct tcattcatcc atgtcttaac
     6481 aggacccagc atccaatttg cagcagcctt aaaattattt gtatgcttta tgatactttc
     6541 aaaataatct gcaaatgctt tttcttctgt aagaacagct gcatcatatt cggagagctg
     6601 gtaagaagaa atatattgct gtattctttg ttctgccaaa gcaggaagac tttttcttat
     6661 cctgtcaatg aattcatcct gtaactgaaa cggtgtaaga tctggttcag caaaatagcg
     6721 gtaatcatct gcatcttctt tatcacgaat ggcaaaagtg gtaccggtat tggcatcaaa
     6781 acttctggtt tgctgattta ttattccccc tttttccagc atatcaatca gcctttttgc
     6841 ttctgcatca accgctctct ttacattccg tatggaattc aggtttttta cctcaaccct
     6901 ggttcccagt tttgtatcgc cttttttacg tacagaaata ttagcatcgc aacgcaggct
     6961 gccttcttcc atattaccat cgcatatctc caggtagcgg accagttttc taacttccgt
     7021 tacatacgca aacacttcat cgctggatcg caggtcaggc tcacttacta tctcaattaa
     7081 gggagtgcct gccctgttgt aatcaacaca ggtgttatcc gggtcaatgt catggaggct
     7141 tttccctgca tcttcttcca gatgaatccg gtttaatctt acttgctttt ccccggagtt
     7201 ggttttaatc tttacaaaac ctccggtaca gatcggtatg gtatgttgcg acacctggta
     7261 gcccttagga agatcggggt agaaataatt tttccgggca aaataattat actgctgaat
     7321 ggtacaatta caggcaagac ccatttttat cgcatactct atcgccttcc ggttcatttt
     7381 gggcaatgtg ccgggatgtc ctaaagtaat cgggctgaca tgggtattgg gttcggcccc
     7441 gaaggcaatg ctgtcaccgc aaaacaattt gctctgcgtt agcaactggg catgtatctc
     7501 caatcccact acaatttcgt atgcactgta atctgtcatt aaacaagtgg gcaaaaatag
     7561 cctatttaag gcagtagaac aggtcaaaag ttaaagcccc gccgtgttca ttgacagcag
     7621 cggggctttg aaaccacaca ccaaaactca aaaaagtatc tacagatcag ctgtttttaa
     7681 tttgcggggc atttttactt ccacattctg tgtttttccg tctcttttca atttgatcat
     7741 tacagaaact ttatccttgc tttccttaac tactctcacc atatcatccg cattattgat
     7801 agccttacca tctacttcgg taattatatc attgttctta atacctgcct tttcagcatt
     7861 actttcatca tccacttcaa tcacttttac acctttgcca tcatcggtat cctgtacgga
     7921 caatccgagt tttggaccac cccctgtcca tgtccaattc tggccaaatg gagcttttgg
     7981 aataggaatc tctttgatct ttggcattat actttcaaaa tccatatcgc caaggtctat
     8041 tttgaaattg tctcccattg accagttttt agcccctttc catttggtaa gctcaacggt
     8101 tgcttttttc tcctttcctt cacggaggta ggtaatcgtt actttatcgc ccggcttatg
     8161 tgctttaatt gcttctgaga ggtcatcagg gcttgcaatc tttttatcat caactttagt
     8221 aataatatca ttttccttta atcctgcttt ttctgctgca ctctccggtg ttatgctctg
     8281 tacttctgcg ccttccgtta ctttctgagt agttacaccc agcatagccc tgttttcatc
     8341 aatactgtaa aaacggaaat tatcgttatt gtcattaaaa ttccaaacac cgccacgggg
     8401 ggctctttct aatgtcaggg cttccacatc tttcaacctg ttcagtttta ccttgatatc
     8461 tccacctttg tctttgtagt cttcaaatgg tttcccgttg atggtaattt tatcaccatt
     8521 aatctcaact actactttat catcagcacc gcttttttta gtgatgatga tctgctgcac
     8581 atcttttctt tcttctttta ctgttttatc cttttcatct ttttgagcca acaaagttgc
     8641 aggggcaaag agggcaatcg ctgcagccaa cacagcattc tttacactta tctttctcat
     8701 acagaaaatg ttttttattt tatctacgtt gaatttcgta gtcaaatata aagacaaaaa
     8761 agaaaatacg aaatgtttcg gaaatgttaa atagccctct tatgccagat ggtattatga
     8821 acggatgaac gggttaatgg cttcataagt aacggccggg tggtcatcga ctatttcttc
     8881 acctatatat ttcataccgc atttttcaag cactttcaga gaaggagtat tttgtggcat
     8941 agccctgccc acaatccttg gcaggttcag tttttcaaat ccatattgca gacaggcaaa
     9001 ggctgcttct gttgcaaaac ccttacccca ggctgatttc ataaaccggt atcccagatc
     9061 tatttcattt ctttccggcc tggctttcaa tccacaccaa ccaataaact cagatcctga
     9121 cttaacatgc acagcccaac ggccatgatt ataaagtgca tattgaggca atatgacctg
     9181 ttccaaaatt tggcaagcat gttcaatatc ccgaaccggg tcgcctgtat actttgttac
     9241 atccggatct ttatttaact cgtaaataag catcccgtca tcctccgtga atgtgcgaag
     9301 aatcagtctt tctgtttcta tgacaatatt catggttctt agcttgcgta tttttactaa
     9361 ccggaaagtg gtattgtctg ggaataataa aacctgaaat ttggcaaata ccttattggt
     9421 taatcattta tggtctatat tattctagta ctgtttgcca ttgtatgcat ctttcttacc
     9481 atatatgcca ttcggatata taaagaagac agtcatatca gggcagaacg caaaagaatt
     9541 ctcgaagaag aagaggaaga cttgtttggt ggcaaactat aaaagctttt tgacggcttc
     9601 aattacttcg cccagttttc ctgcatcctg tccgccagca gtcgccaaag ttttttgtcc
     9661 acccccgccg cctttgatca atggtgcaat tgtttctttg atgatctttc cggcatccag
     9721 gtttttggct gtgaccaccg tttctgaaat accaaccgct acaaatggtt ttcccccaag
     9781 attgacgcag agcacagcca catggtcacg aagatgattt ttcaaatcat agcagagttt
     9841 tttcaaagca tctgcgctgc tcacctcgat aatatcccca acaaagttta ccccgttgat
     9901 gatttcgtct ttttgtaaaa gctcattacg aatagccacc aactgccttg cttccatggc
     9961 ctcaaggtgc tttttcagtt ctgcattctc cacttgcagg ttctcaattg ctttttgaat
    10021 atcctttgga ttcttcaatg cttctttgag cttatccagc agcagcaagt tttcatttac
    10081 atattcctcc gcctgtttgc cacagactgc ttccaccctt cttacacctg ctgctaccgc
    10141 tgattcgctg gtcagtttga aaaggcctaa ctccccggta gagccgacat gggtgccacc
    10201 gcataattcc acagaatatg atgggtcaat tatgaccacc cggactacat cgccatactt
    10261 ctcgccgaac aaggccatag cgccgagctt caatgcctcg tctttaggta atgattttat
    10321 gacgacagga atattttccc tgatcttctc atttacaata gcctcaattt tcactatttc
    10381 ttcatctgtt actttggcaa aatgagaaaa gtcaaaccga agatgtttct cattcaccaa
    10441 tgagcccttt tgcgcaacat gggttcccag cactttcctt aacgctgcat gcaataagtg
    10501 agtagccgaa tgatgcaagg ctgtattctt tcttttgacc gcatcaactt ttgcgattat
    10561 ttcaccactt acttcctcag gaaatctttc ggtgaagtgg ataataaggt cattgtcctt
    10621 tttagtatca gttacttcaa ccgtttcaga gccgatctgc aaccaaccct tatcgccaac
    10681 ctgtccgccg ctttcggcat aaaaaggcgt agtatccagt a
//
LOCUS       DAORLS010000082        10721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3311_length_10721_cov_2.018751, whole genome shotgun sequence.
ACCESSION   DAORLS010000082 DAORLS010000000
VERSION     DAORLS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_10721_cov_2.018751"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..>238
                     /locus_tag="PK977_07910"
     CDS             50..>238
                     /locus_tag="PK977_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18078.1"
                     /translation="MYKSWWKIAAVLLLIYTVIAGFLGPVPKLQLLGETIRNLYFHVC
                     MWFAMMILFTVSVVNAIRY"
     assembly_gap    239..396
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <397..748
                     /locus_tag="PK977_07915"
     CDS             <397..748
                     /locus_tag="PK977_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18079.1"
                     /translation="ARDPKLIGAAIALLVYFAYLILRDSIQDIDKKARISAVYNIFAY
                     AILFPSIWILPRILPSLHPGNEDNPALDVKNDISGNMRIVFYPAVIGWTLLAVWITTL
                     KIRLTLLKEKKMMP"
     gene            745..951
                     /locus_tag="PK977_07920"
     CDS             745..951
                     /locus_tag="PK977_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HRF18080.1"
                     /translation="MMKYCKKITALFTMILLSTALFAQDKDVSVADTMRSNGRIYVVV
                     AVVLTILIGLILYIIRLDRKITKL"
     assembly_gap    1052..1143
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1144..2433
                     /locus_tag="PK977_07925"
     CDS             <1144..2433
                     /locus_tag="PK977_07925"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRF18081.1"
                     /translation="FPIKRDDGSIEVIEAYRVQHSHHKTPCKGGIRFAAEVNQDEVMA
                     LAALMTYKCTIVDVPFGGAKGGIKIDPKKYSPFELEKITRRYTSELIKKKFIGPGTDV
                     PAPDYGTGEREMSWIMDTYSAMHPGEIDAAGCVTGKPISQGGVRGRREATGLGVFFGL
                     REVCNMPDVMGKLGLTTGVEGKKVIVQGLGNVGYHSAKFFQDAGSKIIALAEYEGAIW
                     SDKGLDVDQVFQHRKKTGSILNFPGANNFAKSSDALEMECDILIPAALENVIDGENAP
                     RVKAKIIGEAANGPLTPEADEVFTQKGILVVPDMYLNAGGVTVSYFEWLKNLSHVRYG
                     RLEKRFTENLNTRILSQMEELSGKKVSDNERELIMHGPDEVDLVYSGLEETMINATRE
                     IMKTWREIPEIPDMRTAAYVVAINKVATAYAELGIFP"
     assembly_gap    2738..2750
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2751..3335
                     /locus_tag="PK977_07930"
     CDS             <2751..3335
                     /locus_tag="PK977_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18082.1"
                     /translation="KSAKMTGIATLQGMDLPITIQVINGKAFRIDVEVMGQSVVNSYK
                     DGKGWKINPFGGAETATDVEGAELLDYKAQASLSNSLMDYKARGHQVELLGQEDVDGK
                     KAFKIKLTSKDDGKVTTFFINSEDNMLLKSVSSREMQGQTVDVDTYYTDFKEFGGIKI
                     AMTRSQKIMGQDFQTITLNNVELNVPVDEKVFDK"
     assembly_gap    3538..3579
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3580..3829)
                     /locus_tag="PK977_07935"
     CDS             complement(<3580..3829)
                     /locus_tag="PK977_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRF18083.1"
                     /translation="MVVINYPEPAFRLRKKEETEEIFDSQRKKWIALTPEEWVRQNFV
                     QFMVSVKKYPASLIAVEKELKLGELKKRFDILVYNSRHE"
     assembly_gap    3915..3964
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3965..4640
                     /locus_tag="PK977_07940"
     CDS             <3965..4640
                     /locus_tag="PK977_07940"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRF18084.1"
                     /translation="SNYVKMFAEWNNVPVIGEGKPMQCATAQNITIINFGMGSPTAAT
                     IMDLLSAIQPKAVLFLGKCGGLKKRNKLGDLILPIAAIRGEGTSNDYFPAEVPALPAF
                     ALQKAVSTTIRGYEVDYWTGTVYTTNRRVWEHDEPFKEYLQKIRAYAIDMETATVFTV
                     GFYNKIPTGALLLVSDQPMIPEGVKTEESDKTVTAMFVERHLKIGIDSLKELINDGQT
                     VRHLKF"
     gene            4653..5027
                     /locus_tag="PK977_07945"
     CDS             4653..5027
                     /locus_tag="PK977_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18085.1"
                     /translation="MKLFLLLAGLSVLTLSVSDCNDKKNTEKYKGKLEIAGICMNYTI
                     RITEGEIDTAKISANWTDEVTNKNYINVFRLGSPCDFPSTIKQGDEFYFIIDTAKPKE
                     CMVCMAYYPTPPRSLNIKVVEK"
     gene            5027..>5293
                     /locus_tag="PK977_07950"
     CDS             5027..>5293
                     /locus_tag="PK977_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF18086.1"
                     /translation="MQPLLSINDLSVDFIAESGITNALKNISFAVNRGEVVALVGESG
                     SGKSVTSLSILQLLPSPPVRYSSGEILFSEDGEQVINLLHRKRNE"
     assembly_gap    5294..5324
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5358..>5714
                     /locus_tag="PK977_07955"
     CDS             5358..>5714
                     /locus_tag="PK977_07955"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF18087.1"
                     /translation="MTSLNPVLTCGDQVMEAILLHKKIPKETAKQKTIEWFEKVKLPD
                     PKAMFNGYPHQLSGGQKQRVMIAMAMSCEPALLICDEPTTALDVTVQKTILQLIKELQ
                     KQSGMGVIFITHDLGVV"
     assembly_gap    5715..5726
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5727..6755
                     /locus_tag="PK977_07960"
     CDS             <5727..6755
                     /locus_tag="PK977_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF18088.1"
                     /translation="ADRAVVMYKGEIVEQNMVKNIFTNPQHPYTKALLACRPVNHERG
                     KRLPVVSDFMDDLRTETVKKDIATTILSTVSTKTDLLLSVQQLSVWYPARRTLFGRAH
                     EFTKAVDSVSFDVYKGETLGLVGESGCGKTTLGRTLLRLVEPTNGKIIYNGADLTARN
                     KDELHSLRKEIQIIFQDPYSSLNPRLTIGSAISEPMKVHGLFTSDKQRKEKVTALLEK
                     VNLRPDHFNRYPHEFSGGQRQRIVIARALALNPSFIVCDESVSALDVSVQAQVLNLIN
                     DLKREFGFTVIFISHDLSVVRYISDRIMVMNKGRIEESGPADNIYFNPQSVYTKNLIA
                     AIPKGIIA"
     gene            complement(6752..10591)
                     /locus_tag="PK977_07965"
     CDS             complement(6752..10591)
                     /locus_tag="PK977_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRF18089.1"
                     /translation="MRIIPIIFLLALSVCSVNAQPVIKAKKYPSLLWEISGKGLKKPS
                     YLIGTMHVSSKLAFNLPDSFYYAIRKAEVVALETNPETWQDDLNKYDLSGGKSYNDGY
                     KYSLYSSMPSDYLSINTLKFFKYDNKIEKALYSNPSTINSLLYRTYGNESSDFEEDTY
                     LDMYIFQCGKKWGKKVTGVEDYGESMKLMAEAYRDGAKDKNRKERSYGDAGDEYSEEK
                     LEEAYRTGNLDLLDSINKYNSESAAFDEKFLYQRNEIQANSIDSIIASGSILFVGVGA
                     AHLPGERGVIEILRRKGYKLRPVKMGERASKEKELVDKIRVPVTFRTASAADGAYRVD
                     IPGTFYKMGDDVAQDQRQYADMANGSYYMVTRIMTNAWMWNHSSEDVYKKVDSLLYEN
                     IPGKIISKTAIVKNGYKGFDITNRTRRGDLQRYNIFITPFEVIFFKMSGNGDYVKLGD
                     EAKRFFGSIQLKEYKNGGPYKKYSPAYGGFAVDLPHEPYIGNDGSWIYDAEDKAAGIN
                     YRVIRSDIHNYRFAEEDSFDLGLMDESFMASEFMDTQLLRKQTSYNGYPALDGKYKDK
                     AGKIYWARFIIQGPHYYSLIAYGKEENLAMQQFFNSFELKPLVYGEAKIQKDTSLYYT
                     VTTPYFPKDEKIKIDVKEYNYFGGEDDEDEESEDDLLESGAYRNKIIANDSTGEKIFI
                     SFYRSPRYYYVKDSAKLDLENQLSVEGSVQGDSNWVVRWKNKSELPNKFKVWEAIVTE
                     KGSSRIVHSKAFYKDGIGFSLMTQSDTLSAPSEFVKHFFNSFTPADTLKGINPLEKKS
                     KLFFEDFMSTDTVLRKRAVKHIGDIDLDSSDFPMLKKAISLLNWNDKKYLDTKKEFIN
                     KLGGIKTIASAQYLKELYYALDDTVQLQYAVLENLLQHRTSYAYTIFRDIVNAEPPVL
                     DFATSDYSPYEALKYLNLYRTGGFSYTNGRFLDELSDSLKLTKTILPDLLPLLNLEDY
                     KSSIMKLLGQMVDSSLVKPGDYEMYFSKFLIEAKQELKKQAIAEKKKAIQKAEESKTD
                     KKPISYFRSGDEDDYGNDDLNLYATLLLPFWETNISVQPFIQQMLRSNDKRLKYNTML
                     LLLDKNKPVPDTLFGYFSKLEEYRYELYTDLKKRKKLNKFPPASLNHLDLGKSALLKS
                     ESYDKPDSIVYIDRLQAEYKEKKGFIYFFKYKAKKDDLTWKLATVGLVPLDPAQFEFE
                     DSSNIKIPFFYSPLLPSYGFNYKKYDFTELTDTKLKDDEPVNKQLNKELKKMLYSRRK
                     SAKEFYGKDNNRNEYAY"
BASE COUNT         3069 a         2174 c         2058 g         3022 t
ORIGIN      
        1 aggccggtaa tctttaccag ccaccccgat gactgatcga aaaaactata tgtacaagtc
       61 ctggtggaaa attgcagcag tattgttgct catttacact gtaatagctg gcttcctcgg
      121 tccggttccc aaattgcaat tacttggaga aacaatcagg aacctctact tccatgtttg
      181 catgtggttc gccatgatga tacttttcac tgtttccgtg gtgaatgcca tccggtatnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc agggatccta aattgatagg
      421 tgccgccatt gcccttttgg tctatttcgc ttacctcata ctgagagatt caatacagga
      481 tatagacaaa aaagccagga ttagtgctgt atataacatt tttgcctatg ctatactgtt
      541 cccttccatt tggatattgc caagaatact ccccagcctg catcccggca atgaggacaa
      601 tcctgccctt gatgtgaaaa atgatatcag cggcaatatg cgaattgtat tctatcctgc
      661 tgttatcgga tggaccttgc tggctgtttg gatcaccaca ctcaagatca ggctgacttt
      721 gttaaaagaa aaaaagatga tgccatgatg aaatattgta aaaaaataac tgcattattt
      781 acaatgatcc tgctctcaac agcattattt gcccaggaca aagatgtatc tgttgcagac
      841 actatgcgca gtaatggccg tatctatgtg gtggtagctg ttgtgctaac aattttgatt
      901 ggcctgattt tatacataat aagacttgac agaaagatta caaaactgtg aagagaaagt
      961 aactaaagag gttgattatt acaataacga ttgctaaaac aaaaacagaa ctcatgtcaa
     1021 acacaaacta cagtttcttt ggcgctgttg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnttcccga tcaaaagaga tgatggaagc attgaagtaa tagaagccta ccgtgtgcag
     1201 cactcacacc ataaaactcc ctgcaagggc ggtatccgtt ttgctgccga agtgaaccag
     1261 gatgaagtaa tggcacttgc agcgttaatg acgtataagt gtaccattgt tgatgttcct
     1321 tttggtggag ctaagggtgg tatcaagatt gaccccaaaa aatattctcc gtttgaactg
     1381 gaaaaaatca cccgccgtta tacatctgaa ctgatcaaaa agaaattcat cggacccggc
     1441 actgatgttc ctgctcctga ttatggaaca ggggaaagag aaatgtcatg gatcatggat
     1501 acttactctg ctatgcatcc cggagagatt gatgccgcag gatgtgtaac cggcaagcca
     1561 atttcgcaag gtggtgttcg tggccgacgt gaagccacag gactgggagt attctttggt
     1621 cttcgtgaag tatgcaatat gcctgatgta atgggcaaat tgggacttac taccggagtc
     1681 gaaggcaaaa aagtaatcgt acagggtttg ggtaatgttg gatatcactc tgctaagttc
     1741 ttccaggacg caggttcaaa gatcattgct cttgctgaat acgaaggtgc catatggagt
     1801 gataaaggac tggatgtaga tcaggttttc caacacagaa agaaaacagg atctatccta
     1861 aatttccccg gtgccaacaa ttttgccaaa agctctgacg cattggaaat ggaatgtgac
     1921 atcctgatac ccgctgcatt agaaaatgta atagacggcg agaatgcgcc ccgtgtaaaa
     1981 gctaaaatca taggcgaagc cgccaacggg cccctgacac cggaagcgga tgaagtgttt
     2041 actcaaaagg gaatactggt tgtaccggat atgtacctga atgcaggtgg tgttaccgtt
     2101 tcttattttg agtggttgaa aaatcttagc catgtacgct atggaaggct ggaaaaacgc
     2161 tttacggaaa accttaatac ccgtatcctt tcccaaatgg aagaattaag cggcaaaaaa
     2221 gtgagtgata atgaaagaga actgatcatg cacggaccag acgaagtaga cctggtatac
     2281 agtggcctgg aagagacaat gatcaatgct acccgtgaga tcatgaaaac atggagagaa
     2341 atacctgaaa tacctgatat gcgtacggca gcttatgtag ttgctattaa taaagtggct
     2401 actgcttacg ctgagctggg tattttccca taatcaagcc aattgcttac tatttcaaaa
     2461 tcccatttgc aaatgcaggt gggatttttt ttacagtggt actaaaaaat ccattaaaag
     2521 ggcagttcaa aaagggtgtt cagcctgcca ttaagggcta aatcactact cttcctaatt
     2581 ctcctacctt tgcctatcaa aaaatcaaaa aaaccatgaa acgattctta acagccgtac
     2641 ttggcttcgc ttttctttct gtacaggcgc agacggccga tgaagtgatc agtaaataca
     2701 gtgctgctat gggcggactg gatgctttca acaatgtnnn nnnnnnnnnn aaatccgcta
     2761 aaatgaccgg aattgcaacc ctgcagggga tggacttgcc catcaccatc caggtgatca
     2821 atggcaaagc tttccggatt gacgtagaag taatgggcca atcagtagtg aacagttaca
     2881 aagacggaaa aggctggaaa atcaatccgt ttggcggcgc cgaaaccgct accgatgtag
     2941 agggggctga attactggat tataaagcac aggccagtct tagcaacagc ctcatggatt
     3001 ataaagcaag aggtcaccag gttgaactcc tgggccagga agatgtggac ggtaagaaag
     3061 cttttaaaat taaactaacc agcaaagacg atggaaaggt gacaaccttt ttcattaatt
     3121 cagaagacaa catgctattg aaatcagtta gtagcaggga aatgcaaggg caaaccgtag
     3181 atgtggatac ttattacacc gactttaaag agtttggtgg aataaaaatc gccatgaccc
     3241 gttcacaaaa gataatgggc caggattttc aaaccattac cctcaataat gttgaactga
     3301 atgtccctgt agatgaaaaa gtgtttgata aataaatttg accagcaata ttctaaaagc
     3361 catttgaatt caaatggctt ttttattcat gatcaggtaa tatactgatt tcgtgaaggc
     3421 ctgtttctcc tctttgccaa cccatagtat aattgccgtt tgtaatgata ataaaagaaa
     3481 caggtatact tatattgtat cttaaagcct gttgcaatac ttcttatcca gtagaacnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttcatgccgg ctgttgtata
     3601 caaggatatc aaatcgcttt ttcagctctc cgagttttaa ctctttttct actgctatca
     3661 atgaggctgg atatttttta actgaaacca tgaactgaac aaaattctgc ctgacccatt
     3721 cttctggtgt aagagcaatc cattttttac gctgggagtc aaatatttcc tccgtttctt
     3781 cctttttgcg taaacgaaaa gccggctcgg gatagttaat tacaaccatg cttcaaatgt
     3841 aattgattaa atttgtcagc atgaagacaa aagaagaaat agtacaaaac tggttacccc
     3901 gttatacggg agaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnncagtaa ttatgtaaaa atgtttgccg agtggaataa tgtaccggtt attggtgaag
     4021 gcaaacccat gcaatgcgcc acagcgcaga atattactat cataaacttt ggaatgggca
     4081 gccctactgc cgcaaccata atggatttgt tgtcagcaat acaacccaaa gctgttttat
     4141 tccttggtaa atgcggcggc cttaaaaaaa ggaataaact tggagacctc atacttccga
     4201 tcgctgccat aagaggtgaa ggtacatcca atgattactt ccctgcagaa gttcctgcac
     4261 ttcctgcctt tgctttacaa aaggcagttt ctactactat tcggggttat gaagtcgatt
     4321 attggaccgg caccgtatac actactaacc gccgggtttg ggaacatgat gaacctttca
     4381 aagaatacct gcaaaaaatc agggcttatg ccattgatat ggaaacggct acagtcttta
     4441 ccgttggctt ttataataaa atacctaccg gcgctttatt attggtaagc gatcagccca
     4501 tgatacccga aggcgttaaa accgaggaaa gtgacaaaac cgttactgca atgtttgtgg
     4561 aaagacatct taagattggc attgactcac tgaaagaact tatcaatgac ggccaaacag
     4621 taaggcacct gaaattttaa agtagttatt aaatgaaact attcctgctt ttagctggct
     4681 tatctgtact gaccctttcg gtcagcgatt gtaatgacaa aaaaaatact gaaaagtata
     4741 aaggcaaact ggaaattgca ggcatctgca tgaactacac tatccgcatt accgaaggag
     4801 aaattgatac agctaagatc tcagcgaact ggacagacga ggtaacgaat aaaaattata
     4861 tcaatgtatt caggcttggt agcccatgcg attttccttc cactattaaa cagggtgatg
     4921 aattctactt tatcattgat actgccaaac ccaaagaatg tatggtctgc atggcatatt
     4981 accctacgcc accgaggtca ttaaatatta aagtggttga aaaataatgc agcccctgtt
     5041 aagcataaat gatctgtcgg ttgattttat cgctgaatca ggtattacaa acgcattaaa
     5101 gaatatttcc tttgctgtaa atcgtggaga agtagtcgct ttggttggcg aatccggctc
     5161 cgggaaatca gtcacttctc tttccatttt acaactattg ccctcgccac ctgtccgtta
     5221 cagcagcgga gaaatattat tttctgaaga tggggagcag gttattaacc tgctccaccg
     5281 aaaaagaaat gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggacat aagatcgcca
     5341 tgatattcca ggaaccgatg acctcgctca acccggttct aacctgtgga gaccaggtta
     5401 tggaagccat actactgcat aaaaaaatac caaaggaaac agcaaaacaa aaaaccatcg
     5461 agtggtttga aaaggttaag ctgcccgacc ccaaggccat gttcaacggg taccctcacc
     5521 aactcagtgg cggacaaaag cagcgagtaa tgattgcaat ggctatgagt tgtgagccag
     5581 ctcttcttat ctgtgatgaa cccaccactg ctcttgatgt tacagttcaa aaaacaatac
     5641 tccagctcat caaagaacta cagaagcaat ccggcatggg tgtcatcttt ataacacatg
     5701 atttgggagt agtgnnnnnn nnnnnngccg accgtgccgt agtgatgtat aaaggtgaaa
     5761 ttgtagagca gaatatggtt aagaatattt tcaccaatcc acagcatcca tatacaaaag
     5821 ctttgctggc gtgcaggccg gtcaaccatg aaagaggaaa aaggcttccg gttgtcagcg
     5881 actttatgga tgatctgagg acagagacag taaaaaagga tatagcaaca actatcttat
     5941 caactgtatc aacaaaaaca gatctacttc tatccgtaca gcaactctca gtttggtatc
     6001 cagcccgaag aactttattt gggagagctc atgaatttac caaagccgtt gatagtgtca
     6061 gttttgatgt ttacaaagga gaaacccttg gcttagtggg tgaaagtggt tgtggcaaaa
     6121 ctactcttgg cagaacactg cttcgcctgg tagagcctac caacggaaag atcatctaca
     6181 atggtgccga ccttacagca agaaacaagg acgagcttca ctctctcagg aaagagatcc
     6241 agattatttt ccaggatcct tattcttcat taaatcccag gctaactatc ggttcagcca
     6301 tttcagaacc aatgaaagta catggattat ttaccagtga taaacaacga aaagaaaaag
     6361 taaccgcatt actggaaaaa gtgaacctga ggccagatca cttcaaccgc tacccgcatg
     6421 aattcagtgg agggcaacgc caacggattg ttattgcccg tgctttagca ttaaatccat
     6481 cctttatcgt ttgtgatgaa tctgtttcag cacttgacgt aagtgttcag gcacaggtgc
     6541 taaacctgat caatgatctt aaaagagagt ttggctttac tgtgatcttt atctctcatg
     6601 acttgtcagt tgtacgctac atcagcgatc gcataatggt catgaacaaa ggaaggattg
     6661 aagaaagcgg tcctgctgat aacatttatt tcaaccctca atccgtttat acaaaaaatc
     6721 tcattgccgc aatccccaaa ggcattattg cttaataagc atattcgttt cggttgttgt
     6781 ctttaccata aaactcttta gcactttttc tgcgggaata aagcattttt tttaactcct
     6841 tgttcagttg cttgttcacc ggttcatcgt cttttaattt ggtgtcggtg agctctgtaa
     6901 aatcatactt tttatagtta aaaccgtacg atgggagtaa tggcgaataa aagaaaggta
     6961 tcttaatgtt actgctgtct tcaaactcaa attgggccgg gtctaatggc accaggccta
     7021 cagttgccag cttccaggtc agatcatctt ttttagcctt gtatttaaag aaatagatga
     7081 agcctttctt ctccttgtac tctgcctgta acctgtcaat gtatacaatt gaatctggtt
     7141 tatcataaga ttcacttttc aacaatgcac tttttccaag gtcaagatgg ttgagacttg
     7201 caggaggaaa tttgttgagc tttttccgtt tttttaaatc ggtatataat tcatacctgt
     7261 attcctccag cttactgaaa tacccaaata atgtatctgg cactggttta tttttatcaa
     7321 gcagtaaaag catggtatta tacttcaacc ttttgtcatt cgatcttaac atttgctgga
     7381 tgaacggttg aacagaaata tttgtttccc aaaaaggcag cagcagcgtt gcatacaggt
     7441 tcaggtcatc atttccataa tcatcttcat ccccagacct gaaataagaa ataggctttt
     7501 tatcagtttt actttcctcg gccttttgaa ttgctttttt cttctctgca atagcctgtt
     7561 tttttaattc ttgtttggct tcaattagaa atttgctaaa atacatttca taatctccgg
     7621 gttttaccag actactatcc accatctgtc ccagcagctt catgatactg cttttataat
     7681 cttccaggtt taacagtggc aaaagatcgg gcagaatagt cttcgtcagc tttaaagaat
     7741 ctgaaagctc atcaagaaat ctgccatttg tataactaaa gcccccggtc ctgtaaagat
     7801 ttaaatattt taaagcctca tatggagaat aatctgaagt agcaaaatca agcacgggcg
     7861 gctctgcatt cacaatatcc ctgaaaatag tatacgcata gctggttctg tgctgtaaca
     7921 ggttttccaa cactgcatac tgtaactgaa cggtatcgtc cagcgcataa taaagttcct
     7981 tcagatattg ggctgaggca atcgttttta tgcctcccag tttattgata aactctttct
     8041 tcgtatctag atatttcttg tcgttccagt taagcaagct gatcgctttt tttaacatag
     8101 gaaaatcaga tgaatccaaa tcgatatccc cgatatgttt taccgccctt ttgcgtaata
     8161 ccgtatcagt gctcataaaa tcttcgaaga acagtttaga ttttttctcc aatggattga
     8221 tgcctttcag tgtatcggca ggtgtgaaag aattaaaaaa atgttttaca aattcgcttg
     8281 gggcagaaag ggtatcagat tgcgtcatca atgaaaaccc gataccgtcc ttataaaatg
     8341 ctttagaatg aacaatcctg ctgctgccct tctccgttac gatagcttcc cagactttaa
     8401 acttattggg caactctgat ttattcttcc acctcactac ccagttagaa tcgccctgaa
     8461 ctgaaccttc tacggaaagc tggttttcca ggtcaagctt tgcgctatct tttacataat
     8521 aataacgggg agaccggtag aatgaaataa aaattttctc accggtagag tcattggcaa
     8581 taatcttatt tcgatacgca ccgctttcaa gtaaatcatc ttcagactcc tcatcttcgt
     8641 catcttcgcc accaaaatag ttgtactctt ttacatcaat ttttattttt tcatctttgg
     8701 ggaaataagg agtagtaacg gtataataca atgaagtatc cttctggatt tttgcttctc
     8761 cgtaaaccaa cggtttcagt tcgaatgaat tgaaaaactg ctgcatcgcc agattttctt
     8821 ctttgccata tgctatcagt gaatagtaat gtggcccctg gatgataaat cttgcccaat
     8881 agattttacc tgctttgtct ttatacttac catccagcgc cgggtagcca ttatagcttg
     8941 tttgtttacg caacaactgg gtatccataa actcagatgc cataaaactc tcatccatca
     9001 ggccaaggtc aaaactatct tcctctgcaa agcgatagtt atgaatatct gaacgaatga
     9061 cccggtagtt tattccggcc gctttatctt ctgcatcata gatccagctc ccatcgttgc
     9121 ctatatatgg ttcatgtggc agatcaactg caaacccgcc atatgcgggg gaatattttt
     9181 tatacggccc gccgttttta tactccttca actgaatgct gccaaagaac ctttttgctt
     9241 catcacccag ttttacataa tcgccattac cactcatctt gaaaaagatg acttcaaaag
     9301 gtgtaatgaa aatattataa cgctgcaaat cacctctgcg ggtccggttg gtgatatcaa
     9361 aacccttata accattttta acaatggctg ttttggaaat gatcttcccg ggtatatttt
     9421 catataacag gctgtctact tttttataaa catcctctga actatgattc cacatccagg
     9481 cattggtcat tatcctggtt accatgtagt aacttccgtt agccatatct gcatactgcc
     9541 gctgatcctg ggctacatca tctcccattt tataaaacgt tccgggtata tccaccctgt
     9601 aagcaccatc tgctgctgac gctgtgcgaa aagtaacggg cactcttatt ttatcaacca
     9661 gttctttttc tttactggcc ctctctccca ttttgacagg acgtagtttg taacccttgc
     9721 ggcgcagtat ttcaatcacc cctctttccc cgggaagatg tgcagccccg acaccgacaa
     9781 acagtatcga accgctggct ataatggaat caatcgaatt tgcctgtatt tcgtttcgct
     9841 ggtacaaaaa tttttcatca aatgccgccg attcgctgtt gtatttgttt attgaatcga
     9901 gcaaatcgag attgccggtg cgataggctt cttccaattt ttcctcgctg tattcatctc
     9961 ctgcatcgcc atagcttctt tcctttctgt tcttatcttt agctccgtcc ctataagcct
    10021 ctgccatcaa tttcatgctc tcaccataat cttctacacc tgtaactttt ttaccccatt
    10081 tcttaccaca ctgaaatata tacatatcca aataagtgtc ttcttcgaaa tcactggact
    10141 cattgccgta agtccggtat aacaaactgt tgatggttga aggattacta tataaagcct
    10201 tttcaatctt attgtcgtat ttgaaaaact taagggtatt gatcgacaga taatcggacg
    10261 gcatggatga atataaactg tatttatatc cgtcattata tgatttaccg cccgagaggt
    10321 catacttatt caggtcatcc tgccatgtct ctgggtttgt ttccaaagca actacttcgg
    10381 cttttctgat agcataataa aacgagtcag gcaggttaaa tgcgagttta ctgcttacat
    10441 gcatggtgcc aatcaggtat gaaggctttt ttagcccctt gccggaaatt tcccataata
    10501 gactggggta ttttttagct tttattacgg gctgggcatt tactgaacaa acggataagg
    10561 ccagaaggaa aataatcggg ataattcgca tcataaaggg ttatagaccg gcaattcccg
    10621 ggttttgatt tgatgtaaac aaatttaaga tgccggaggc cattgaaaag cataaaatta
    10681 ttttgaaatt cagcgactta acccccgaat ccttttgtga a
//
LOCUS       DAORLS010000083        10619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3352_length_10619_cov_2.194529, whole genome shotgun sequence.
ACCESSION   DAORLS010000083 DAORLS010000000
VERSION     DAORLS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3352_length_10619_cov_2.194529"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PK977_07970"
     CDS             <1..1511
                     /locus_tag="PK977_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18090.1"
                     /translation="IKLDQPSLTVQKNGINGGIVFQWNGNENELNAIQLNDISINKSK
                     VSISSAFFSASGIKFRPPNGTLFNSGNATSSGTITDVQLQYDISKKLQWSSLLSVFNC
                     NNLALNNVGKKEGKLALHSISLKNLKLNSDFLKNLKNLTEFSKDFEVSQFTGSYADTA
                     ITVKWFNAGLERTIKTFKLDSFSYQPALPRDSFLAMQPYQVDYITAKSGAVEISPLDI
                     NQFINKNELSAGKIIVNDFYFTDYKDKRLPFNAGIIKPLPVGLLKKLRWLISIDTVFL
                     NNAAVEYTEIIPPANTALYVPVTRMNARLLNLKNYQITPNDSLDIQSTGYLLDTLWVK
                     LKVRQSYTDSLEGFWMTLQAKPADLRQLNPIFIPAASMKLESGNLDTMILEVIGREYL
                     AQGEMQLLYHDLKISLLKDGDTSKRSLGSRIKNFIANTFVIRKRNKSRTSPVFFIRNR
                     DKSAVNYLVKIVLTGFQASIGGKNPKKEMKKFRLELKKRQLPPIEPEESGRE"
     gene            1574..>1783
                     /locus_tag="PK977_07975"
     CDS             1574..>1783
                     /locus_tag="PK977_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18091.1"
                     /translation="MPIKTTFLCGLLCLASVLTYCQTQDDFNTKLSDIYSNAADKKKA
                     LSIAKELYNIVERKKELQTYSNYYLL"
     assembly_gap    1784..1849
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1850..>3562
                     /locus_tag="PK977_07980"
     CDS             <1850..>3562
                     /locus_tag="PK977_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18092.1"
                     /translation="MNAMVGLNNEMKADTSNPSLAWATLYFPALFKNEDPENASKALA
                     FINRNPDLKNFTNYTYIAYSFERNNDFTKARENYEEALKFVTDEKKDYHSYLFYTNFL
                     TRTGDYLKADEFIRKMETLSREATDFFKDGYKSEAMSARVVYYLNIGDYQSYVQASEK
                     NYEYFSIQWQKTNKTACDPYPGIKLVNAAFGKEMLREYDAAEKLWKSRDSANYVWVNC
                     YNKTYPNAKYYPISMYPVYLIKRGKNKLLPKPASYFIKETEDHYNSYKQYADLSATVA
                     KATQLGFLGSSGYPSLFSEIMNKVKHSHDFRESTTPFSNYAYFSMRDRNLQESQKTYN
                     ELFSLNIDWINDIIFTFGEKAFVTFYNAKLKEGYDNYHSFVKLVKDKSTVFYGNAAIQ
                     AYNNLLFTKSISLKGTQKRKEAFLKTNDATVTKLYDEWIDKKQQLIRQYMKKDNPSEA
                     DTSNRFNETVLKSLQEDVNRLENELTIKAKDFKKLLKITPTGWNDVKTQLKEGEAAIE
                     IVRFQWRDQVYYSDTAYYAAYIITKNSQYPEVVYLPDAAADLDNKFYKLYKNNIRLKL
                     DDNES"
     assembly_gap    3563..3625
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3626..4517
                     /locus_tag="PK977_07985"
     CDS             <3626..4517
                     /locus_tag="PK977_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF18093.1"
                     /translation="KIYFSPDGIYHLININTLKNPATKNFVIDELEIHSTTSTADIAF
                     IKPAEKKISDAVLVGRPAYKTNNEIAIAATKDETTRSFVNSFRDNSVPDLPGTEAEVL
                     AIKQELELKKITTKQFVKEQATEDKMYQLKSPGILHIATHGYWSGTGEKATDGYRLFN
                     AMVNSGLLLTGVVNYYSSSSYPDTYDGILTAYEAQNLDLDNTSLVVLSACETSLGYLD
                     AGEGVYGLQRAFRAAGAGSVITSLWKVDDNATKDFMITFYQHFLQTKDKLAAYRLAQK
                     NIKEKYKYPYYWGAFVMVGE"
     gene            4599..>5944
                     /locus_tag="PK977_07990"
     CDS             4599..>5944
                     /locus_tag="PK977_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRF18094.1"
                     /translation="MSKRVIKKVAVLGSGVMGSRIACHFAGVGVQVLLLDIVPKDIAA
                     DAKPAERNKIVNDALTAAIKSNPSPVYHKDVVKKITTGNFEDNMKDIAGCDWVIEVVI
                     ERLDIKQKVFEQVEKYRKPGTLITSNTSGIPIHMMAEGRSDDFKKHFCGTHFFNPPRY
                     LRLLEIIPTPYTDPGIVDFLMNYGDLYLGKTTVLCKDTPAFIANRIGVFGMMAIMNVK
                     EKLKLSVDEIDALTGPVIGRPKSATFRTGDVVGLDTLVKVAKGVADNCPDDEAKDQFA
                     IPAWLEKMIANNWLGDKTGQGFFKKTKGAGGEKEILTLNLDTMEYGPRVKPKFATLET
                     AKPVDDLYTRLKMLVAGTDKAGEFYRLFHYGLFSYISHRIPEISDELYRVDDAMMAGF
                     GWEIGAFESWDVLGVAKTTEAMKAAGYKVAAWVDEMLAAGNKSFYKVEGGKKYCYDP"
     assembly_gap    5945..6016
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6017..>6308
                     /locus_tag="PK977_07995"
     CDS             <6017..>6308
                     /locus_tag="PK977_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRF18095.1"
                     /translation="KQLWKNGSCRLYDLGNDVLGLQWFTKMGSIGGDVLSGIQTAIDK
                     TEKGYKGLVIANEGANFSAGANVGMIFMLAIDQEYDELDMAIRLFQNTMMRAR"
     assembly_gap    6309..6335
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6336..6991
                     /locus_tag="PK977_08000"
     CDS             <6336..6991
                     /locus_tag="PK977_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRF18096.1"
                     /translation="APHGMTLGGGCEMSLHADKVVAAAETYTGLVELGVGLIPGGGGT
                     KEFVLRASDEMHEDEPETITLKNRFLSIATAKVATSAHEGFGLGVYRKGLDEVVPNIN
                     RRIAEAKKSVIEIYDSGYTAPVQRTDIKVLGQSALGALYSGIHAMKTANYATEHDALV
                     AKKLAYVMCGGDLSEPTLVTEQYLLDLEREAFLSLCGEKKTLERIQSVLKSGRPIRN"
     gene            7025..7381
                     /locus_tag="PK977_08005"
     CDS             7025..7381
                     /locus_tag="PK977_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18097.1"
                     /translation="MQVSVVDFAKDRVVYNITLCTREELMNKLNLFFTSEGYKQGSTE
                     GDTTYYTKGNRVLRLLFGAFVKYHKQSLNVKQEGELFSVMLHKDSSGISGGLIGMNQV
                     KKEFARLAEAFKVYFS"
     gene            7404..>8039
                     /locus_tag="PK977_08010"
     CDS             7404..>8039
                     /locus_tag="PK977_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18098.1"
                     /translation="MKKKLFLPVLLAVGMIVQAQTNFSGAYGYSFRPQGDPPATEKNR
                     GPKGSLVLLKMDGDKYRFWLDVETGWPRYHVGETDGTITFVNDTASFDNTFEDATNPC
                     ILSFKITGNIITINSHSTSFNCGFGQGVHADGEYAKLKVQPVFNNDWLKKEYPQSPVA
                     LVTNNRAEIFQDETCLHSFSPKRYFVKGDKFMSIAETEKCIYTEYIPSPGKF"
     assembly_gap    8040..8213
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8214..>8510
                     /locus_tag="PK977_08015"
     CDS             <8214..>8510
                     /locus_tag="PK977_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HRF18099.1"
                     /translation="ETMWNEPSGSLQLAGIMYNVIFRISADYKPGIPDIDIYQNLDPR
                     NNYFRIEEFAHGNISFVDGLGCNSGYFKLENLYKGSTTAAHEYGHTLGLEHPEQ"
     assembly_gap    8511..8671
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8851..9423)
                     /locus_tag="PK977_08020"
     CDS             complement(8851..9423)
                     /locus_tag="PK977_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18100.1"
                     /translation="MNLRITLLLGVTFLWLLSFGQVQSLMPIDTTQKSVEHWQKWLTD
                     LNELGVEKKNDSFFVKQEVLLLLRDSDYRKSVYPGAYSWQRVTELMNTMELKKAFWHL
                     INLYQTDTSRRNMVVGTFVLYDSLMDMDKILVSTFYTYAFTDPQVCRIINGKPDIYRP
                     DLLEKKLRTTREIINYIWMNRKNKQNGSKK"
     gene            complement(9420..>10222)
                     /locus_tag="PK977_08025"
     CDS             complement(9420..>10222)
                     /locus_tag="PK977_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18101.1"
                     /translation="VFDDVNNSLKKTNEFVSFVDKDNRKLGAIRAQSIQDFSYDYFDG
                     QKMLDLAAEFIGIDIVDDFMSIVSGISEMVTDYNNIGVEYSSGNGDYAEWLERIDPRE
                     EISYGDIVAVKGGKITKDLKDAQQIMAVSKQPIVLGNVPDAAKTELGNNIAFMGQIPV
                     KVMGPVMSGDYIVAKSDIPGYGIAVHPKDMQIDDFKLAVGRSWDTNKKEGPKMVNTVV
                     GVHNHDFLNIISGLQQKVSQTDDRLKAIESALNIKTTTTKEPVKKAFK"
     assembly_gap    10223..10232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10233..>10619)
                     /locus_tag="PK977_08030"
     CDS             complement(<10233..>10619)
                     /locus_tag="PK977_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18102.1"
                     /translation="GNAYANVPNPVTQGVETQVNQIRDWIYGNDSFSWDDLINLMPSQ
                     YLTGTVCNLTNYITQRINNAIGLPVGVPPVDVGYQMLSALNSVADVLIDDDWVTDIQG
                     DFVIPGFQFPAIPQLNCTGLPSLSFPV"
BASE COUNT         3235 a         2026 c         2004 g         2781 t
ORIGIN      
        1 aaataaaact ggatcaaccg tcacttactg ttcaaaagaa tggcataaac ggaggcattg
       61 tttttcaatg gaacggaaat gaaaatgagt tgaacgcaat ccaattaaat gatattagca
      121 taaataaaag taaggtttcc attagcagtg cctttttctc cgcttctggt attaagttta
      181 ggccccctaa tggtacctta tttaattcag gaaacgccac tagctccgga accattactg
      241 atgttcagtt gcagtatgac ataagcaaaa aattacaatg gtcttccctg ttatccgtat
      301 tcaattgcaa taaccttgcg ctgaataatg ttggaaaaaa agaagggaag ttggctttgc
      361 attccatttc cctgaagaac ctgaagctta actctgattt cttgaaaaat ttgaaaaatc
      421 taacagagtt tagtaaagat tttgaagtat cccaatttac aggcagctat gctgataccg
      481 ctataactgt aaaatggttt aatgctggat tagaaagaac aataaaaact ttcaagcttg
      541 actctttttc atatcaacct gcattaccaa gagactcatt cctggcaatg caaccttacc
      601 aggtagatta tataaccgca aaatcaggtg ctgttgaaat aagtccgtta gatatcaatc
      661 agtttatcaa taaaaacgag ttatcagcgg gcaaaatcat tgtaaacgat ttttacttta
      721 cagattataa agacaaacgc ctgccgttca acgccggtat tatcaagccg ctacccgttg
      781 gattattaaa aaaattgcgt tggttgattt ctattgatac tgtttttctg aataacgcag
      841 cagtagagta cacagaaata atacctcctg caaatactgc tttgtatgtg ccggttaccc
      901 gaatgaatgc aagactactg aacctgaaaa attatcagat aacaccaaat gatagtcttg
      961 acatacaatc tacagggtac ttactggata ctttatgggt aaagcttaaa gtaagacaat
     1021 cttataccga ttcattggaa ggcttctgga tgaccctgca ggccaaacct gctgacctcc
     1081 gccagctcaa cccgattttt atacctgctg cttctatgaa attagagtca ggaaatcttg
     1141 acaccatgat tctggaggta atcggccgtg aatatcttgc ccagggagaa atgcagttac
     1201 tttatcatga cctgaagata agtctgctaa aggatggcga tacatcaaaa agatctttgg
     1261 gaagtcgcat aaaaaatttt attgcgaata cctttgttat tagaaaaaga aacaaatcaa
     1321 gaacctctcc ggtctttttt attcgcaacc gtgacaaatc cgctgttaac taccttgtaa
     1381 aaattgtatt aaccgggttt caggccagca tcggcggcaa gaatccaaag aaagaaatga
     1441 agaaattccg gcttgagtta aagaaaaggc aattaccgcc aatagaacca gaagaatccg
     1501 gaagagaatg aaaagataaa tctgtagcca gatttatgta tcttaaatgg ccaattacac
     1561 cttatcgttt aatatgccaa tcaaaactac attcctatgc gggttgctat gcctcgccag
     1621 tgttttaact tattgccaga cacaggatga cttcaataca aagctttctg atatttattc
     1681 aaacgcagca gataaaaaaa aggcgctgag tatagcaaag gaactttaca atatagtaga
     1741 aaggaagaaa gaattgcaga cctattccaa ctattactta ctannnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgaatgcaat
     1861 ggtgggtttg aacaatgaaa tgaaggcaga caccagtaac ccctccttgg catgggccac
     1921 actttatttc ccggcattgt tcaaaaatga agatccggag aatgccagca aagctcttgc
     1981 attcataaac cgaaaccctg atttaaaaaa cttcaccaat tacacttata tcgcttactc
     2041 atttgaaaga aataatgatt ttacgaaagc cagggaaaac tatgaggaag cgctgaaatt
     2101 tgtaacagat gaaaaaaagg actatcactc ctatcttttt tataccaact ttctcacccg
     2161 taccggcgat tacctgaaag cagatgaatt catccgtaaa atggaaacgc tttcccggga
     2221 agctacagat tttttcaagg atggatacaa gtctgaagcc atgtctgcaa gagtagttta
     2281 ttacctgaat atcggcgatt atcaatcata tgttcaggct tcagaaaaga attacgaata
     2341 cttctcaatc caatggcaaa agacaaacaa aacagcctgt gacccctacc ctggtatcaa
     2401 acttgtaaat gctgctttcg gtaaagaaat gctgagagaa tacgatgcag ctgaaaaact
     2461 ttggaagagc cgggattccg caaactatgt atgggtgaat tgttataaca aaacataccc
     2521 aaatgcaaaa tattatccta tatccatgta tcctgtttat cttatcaaac ggggtaaaaa
     2581 caagttatta ccaaaaccgg cttcttattt cattaaagaa acagaagatc attataacag
     2641 ttataaacag tatgctgacc tgagtgctac agttgccaaa gcaacacaac ttggtttcct
     2701 gggaagttca ggctatccat cgcttttcag cgaaatcatg aataaagtaa agcattcaca
     2761 tgatttcagg gaatcaacca ctcccttttc caactatgct tatttcagta tgcgagacag
     2821 aaatttacag gaatctcaaa aaacatacaa tgaattattt tctttgaata ttgactggat
     2881 caatgatatt attttcacat ttggagaaaa agcatttgtt actttctata atgccaaact
     2941 caaagaagga tatgataatt accacagctt cgtaaaatta gtgaaagata aatccacagt
     3001 attttatggc aacgctgcca ttcaggccta caacaacctg ctttttacaa aatccatctc
     3061 cttaaaaggg acacaaaaaa gaaaagaagc ctttctgaag acaaatgatg caacggtaac
     3121 aaaactttac gacgaatgga ttgacaaaaa acagcaattg atcaggcaat acatgaaaaa
     3181 agataatcca tctgaagcag acaccagcaa caggttcaat gaaacagttt taaaatcact
     3241 gcaggaggat gtgaacaggc tggagaatga gttaaccata aaagcaaaag acttcaaaaa
     3301 actattgaaa ataacgccaa ccggctggaa tgatgtgaaa acccaattga aagaaggaga
     3361 agccgccata gaaatcgtcc gcttccaatg gcgtgaccag gtctattatt ccgacacagc
     3421 atattatgct gcctatatta ttaccaaaaa cagccagtac ccggaagtag tttaccttcc
     3481 ggatgcggct gctgacctag acaacaaatt ctacaaatta tataaaaata atatccgcct
     3541 gaaactggat gacaatgaat ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnaaaaa tttatttctc gccggatggc atttatcatc
     3661 ttatcaatat aaatacatta aaaaacccgg ctacaaaaaa tttcgttatt gatgaattgg
     3721 aaattcactc cactacatca acagctgata ttgcatttat aaagcctgct gaaaaaaaaa
     3781 tcagtgatgc tgtattagta ggaaggccag cctataaaac aaacaacgaa atagccattg
     3841 ctgcaacaaa agatgaaacc acccgttctt ttgtcaatag ctttcgtgat aatagtgtac
     3901 cagacctgcc gggcacggaa gcagaagttc ttgccattaa acaggaactg gagctaaaaa
     3961 aaatcactac aaagcaattt gtaaaagaac aggcgacaga agataaaatg taccagttga
     4021 aaagcccggg aatattacat attgccaccc atggttattg gtcaggcacc ggtgaaaaag
     4081 cgacggatgg ctatcgtctt ttcaacgcca tggttaattc cggtttatta ttaacaggag
     4141 ttgtcaatta ttatagcagc agcagttacc cggataccta tgatggtata ttaactgcct
     4201 acgaggcaca aaaccttgac ttggataata cttccctggt tgttctctcc gcctgcgaaa
     4261 ccagtttggg ctaccttgac gcaggggaag gcgtttacgg cctgcaaagg gcctttcggg
     4321 cagctggtgc cggcagtgtc attaccagcc tgtggaaagt ggatgacaat gccaccaagg
     4381 attttatgat caccttttac cagcatttct tgcaaacaaa agataaactg gcggcttacc
     4441 ggctggctca aaaaaatatc aaggaaaagt ataaatatcc ttattactgg ggggcttttg
     4501 taatggtggg ggaatgatac tttacttctc ttttcctctt tcacaaaaac gcttatcttc
     4561 gattctaaaa tagttgcata actaactaat tttgtcttat gtcaaaaaga gtcatcaaaa
     4621 aggttgcagt tttaggaagc ggtgttatgg gatcgaggat tgcctgtcat tttgcaggtg
     4681 ttggtgtaca ggtgttgtta ctggatattg taccaaaaga tatagccgct gatgccaaac
     4741 ctgctgaacg aaacaaaatt gttaatgatg cattaacagc tgcgataaaa tcaaatcctt
     4801 cccctgttta tcataaagac gtagtaaaga aaataactac cggcaatttt gaagataaca
     4861 tgaaggatat tgccggatgc gactgggtga ttgaagtagt gatagaaaga ttagacataa
     4921 aacaaaaagt atttgagcag gtagaaaaat accgtaaacc cggaacactg attacttcca
     4981 atacatccgg aatccccatt cacatgatgg cggaaggaag aagtgatgat ttcaaaaaac
     5041 atttttgcgg aacccacttt tttaacccac cccgttatct gcgcttactg gaaataattc
     5101 cgactcccta tacagatccc gggatcgttg acttcctgat gaattacggt gatctttacc
     5161 tgggtaaaac aacagtgcta tgcaaagaca ctcctgcctt tatcgccaat cgtattggtg
     5221 tatttggcat gatggctata atgaatgtaa aagaaaagct gaaactgagt gttgatgaaa
     5281 tagatgcatt gaccggtccg gtcattggcc ggccaaaatc tgccactttc cgcactggtg
     5341 atgtagtagg tttagataca ttggtaaaag tggcaaaagg tgtggccgat aattgcccgg
     5401 atgatgaagc aaaagatcag tttgcaattc ctgcctggtt agaaaaaatg attgccaaca
     5461 actggctggg tgataaaacc gggcaggggt tctttaaaaa gactaaaggt gccggtggag
     5521 aaaaagaaat tcttaccctg aaccttgata ccatggagta tggcccaagg gtaaaaccaa
     5581 aattcgccac cctggaaaca gcaaagccgg ttgatgacct gtatacccgg ttaaaaatgc
     5641 tggttgcggg aacagataaa gccggtgagt tctatcgctt gtttcattat ggtttgttct
     5701 cctatatctc acaccgcatt cctgagatca gtgatgaatt gtaccgggta gatgatgcga
     5761 tgatggcagg ctttggttgg gaaattggtg ccttcgagag ctgggatgtt ttgggtgtag
     5821 ccaaaactac ggaagctatg aaggccgccg gttataaggt tgctgcctgg gtggatgaaa
     5881 tgctggcagc aggtaataaa tcattttata aagtagaagg tggtaaaaaa tattgttatg
     5941 atccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnntaag cagctttgga aaaatggaag ctgccgcctc tacgatttgg
     6061 gaaatgatgt tctcggtttg caatggttca ccaaaatggg aagtatcggc ggtgatgtgt
     6121 tgagtggaat tcagacagcc attgataaaa cagagaaagg ctataaaggg ctggttattg
     6181 ctaatgaagg tgccaacttt tctgccggcg ccaacgtagg tatgatcttc atgctggcca
     6241 tcgaccagga atacgatgaa ctggatatgg ccatccgcct gttccagaat accatgatgc
     6301 gggcccgtnn nnnnnnnnnn nnnnnnnnnn nnnnntagca ccacacggaa tgacgctcgg
     6361 tggcggttgt gaaatgagtt tacacgctga taaagtagtt gccgcagctg aaacctatac
     6421 cggtttggtt gaattgggtg ttggtctaat tcccggtggc ggtggtacaa aagaatttgt
     6481 actcagggca tctgatgaaa tgcatgaaga cgaaccggaa acaatcacat taaagaaccg
     6541 gtttctttca attgcaactg caaaggttgc cacatctgcc cacgaaggtt ttggactggg
     6601 tgtctaccga aaaggattgg acgaagtggt acctaacatc aatcgcagga ttgcagaagc
     6661 aaagaaatcg gtgattgaaa tatatgacag cggatacaca gcccctgttc aaagaactga
     6721 tataaaagta ctgggtcagt ctgcactcgg tgccttgtat tccggtattc atgccatgaa
     6781 aactgctaat tacgcaacag aacatgatgc attagtggcc aaaaagctgg catatgtaat
     6841 gtgcggtggc gatttgagtg aacctacttt agttacagag caatatttac tcgatcttga
     6901 aagagaagca tttctttctc tttgtggcga gaagaagaca ttggaaagga tacagagtgt
     6961 gctgaagagc ggcaggccga ttcggaatta atgctatctt tagaacaacc aaaaaccttt
     7021 caccatgcaa gtatccgttg ttgattttgc aaaagaccgt gttgtttata atattacgct
     7081 atgtacccgt gaagagttga tgaataagct caacctgttc tttacttccg aaggatataa
     7141 acaaggatcc acagaaggtg ataccaccta ctatacaaaa ggcaaccggg tgcttcgcct
     7201 tctttttggc gcatttgtaa aataccataa gcaaagttta aatgtgaagc aggaaggtga
     7261 actgttctct gtaatgctgc acaaagattc ctcgggtatt tccggcggat tgatcggcat
     7321 gaaccaggtg aaaaaagaat ttgcccgcct ggctgaagca ttcaaagtct atttttcctg
     7381 aaattgtttt aaacttatcc cgtatgaaaa agaaactttt tttaccggtg ctcctggctg
     7441 ttggtatgat cgtacaggca caaacaaatt tttccggtgc ctatggatat tccttcagac
     7501 cacaaggcga tccaccggct acagaaaaaa acagagggcc aaagggaagc cttgtattgt
     7561 taaaaatgga tggcgataaa taccgcttct ggctggatgt agaaaccgga tggccccggt
     7621 atcatgtagg tgaaacagat ggaacaatca cctttgtaaa tgatacagct tcttttgaca
     7681 atacgtttga agatgcaacc aacccctgta tcctgagttt taaaataacg ggcaacatta
     7741 tcaccattaa tagccactcc acttctttta attgcggatt tggacaagga gttcatgcag
     7801 atggagaata tgccaaatta aaagtacaac ccgtttttaa taatgattgg ctgaagaaag
     7861 aatacccgca atcacccgtt gcactggtaa caaataacag ggctgaaata ttccaggacg
     7921 aaacctgtct gcattcattt tctcccaaac gatattttgt gaagggtgat aaattcatga
     7981 gcattgccga aacagaaaaa tgtatttata ctgaatacat cccttcaccc ggaaaattcn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagaaaca
     8221 atgtggaatg aacccagcgg cagtttacaa ttagctggta taatgtataa tgtaattttt
     8281 agaatcagcg ctgattataa gcccggtatt ccggatattg acatttacca aaatcttgat
     8341 ccccgcaata attatttccg aatcgaagaa tttgcacatg gtaatatttc ctttgtcgat
     8401 ggcctaggct gtaacagcgg ctatttcaaa ctcgaaaacc tctataaggg ttccaccacc
     8461 gcagcccatg aatatggaca tacattaggg ctggaacatc cggaacaatt nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagatattga caaccttaaa ttgcataaac
     8701 tcagctttgc gaacaacaaa gctatcctgg gtgaatttac caatatctgg catccggatc
     8761 atgccacaat caatcccgga aattgggtat aaaaaagcct cactgctaaa gtgaggccga
     8821 taacatctgc tcaaggttta gactgtgatg ttattttttt gatccgtttt gtttattttt
     8881 tctgttcatc catatataat tgataatctc tcttgttgtt cttaattttt tctccagcag
     8941 gtctgggcga taaatatccg gcttgccatt aataattctg catacctgcg gatcggtaaa
     9001 cgcataagtg tagaaagtac tgaccaatat tttatccatg tccatcaggc tgtcgtagag
     9061 gacaaaagtt cctacaacca tatttcttct ggacgtatca gtttgataaa gattaatcag
     9121 gtgccaaaaa gctttcttta actccattgt attcatcaat tccgtaaccc tctgccagct
     9181 gtaagctccg ggatatactg atttcctgta atcagaatcc ctgagtaaaa gcagaacttc
     9241 ctgttttaca aagaaagaat cattcttctt ttcgactccc aattcattca ggtcagtcag
     9301 ccatttttgc caatgctcaa cacttttttg ggtggtgtca attggcataa ggctctgcac
     9361 ctggccaaaa gacagcagcc acaggaacgt tacacctaga agaagagtta tacgaaggtt
     9421 catttgaatg cttttttaac gggttcttta gttgttgtag tcttgatatt caatgctgac
     9481 tcaatggctt tcaacctgtc atccgtttga ctgacctttt gctgtaatcc tgaaataata
     9541 ttcaggaaat catgattatg aacacctact actgtattta ccatctttgg cccttctttc
     9601 ttattagtat cccatgaacg gccaacagcc agtttaaagt catcaatttg catatccttc
     9661 ggatgaacag caattccata accaggaatg tcactctttg ctacgatata atcaccggac
     9721 ataactggcc ccatcacttt aacaggaatc tgacccataa aagcgatatt attaccaagt
     9781 tcagttttag ctgcatcggg aacattaccc agtacgatcg gttgttttga tacagccata
     9841 atctgctgtg catcttttag atccttagta atcttaccgc cctttacagc tacaatatca
     9901 ccataactga tttcttccct tggatcaatc ctttcaagcc attccgcata gtcgccatta
     9961 ccagaagaat attcaacgcc gatattgtta taatctgtta ccatttcaga aattcctgaa
    10021 acaatactca taaaatcatc cacaatatct atcccgatga attctgctgc caggtccagc
    10081 atcttctgac cgtcaaaata atcatatgaa aaatcctgga ttgattgtgc cctgatagcg
    10141 ccaagcttac ggttgtcttt gtcaacgaat gaaacaaatt catttgtctt tttcaaagag
    10201 ttgtttacat catcaaaaac aannnnnnnn nngaccggaa aagaaaggct gggtaaccct
    10261 gtacagttaa gctgtggtat agcaggaaac tggaacccgg gaataacaaa atctccttgt
    10321 atatcagtta cccagtcatc atctatcagc acatctgcca cactattcag tgcacttaac
    10381 atttgataac ccacatcaac cgggggcaca cctaccggaa gaccaatggc attattaatc
    10441 ctctgcgtta tataatttgt aaggttacac actgtacctg tcagatattg agaaggcatc
    10501 aggtttataa ggtcatccca gctgaagcta tcatttccat atatccagtc ccttatctgg
    10561 ttaacttgtg tttctactcc ttgagttacc gggttgggca cattggcata ggcattacc
//
LOCUS       DAORLS010000084        10360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3453_length_10360_cov_2.084425, whole genome shotgun sequence.
ACCESSION   DAORLS010000084 DAORLS010000000
VERSION     DAORLS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3453_length_10360_cov_2.084425"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK977_08035"
     CDS             complement(<1..368)
                     /locus_tag="PK977_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRF18103.1"
                     /translation="MRFFFSIAVLLCSVLLQAQVSFKTVVPQQPLVAGESFQVQYILE
                     DADNASNLKPPIFKNFRYVAGPNLYTGSVPSVHGSKPLRNFVYTLEATRPGRFMIPGT
                     TIVADGQLLQSSDVWVKVIT"
     gene            complement(492..1190)
                     /locus_tag="PK977_08040"
     CDS             complement(492..1190)
                     /locus_tag="PK977_08040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF18104.1"
                     /translation="MNVVITGASKGIGKAIAEIFAAYGHHLFLCSRGEVALYKTMEEL
                     MTKYPAAIIKAKPFDLSRKEEAKAFGAWCLEYCVPDVLVNNAGLFEPGSVHNEPEGTL
                     ESQLAVNLHSAYHVTRSVLPWMMEKRSGHIFNMCSIASLDAYANGGAYSISKFALYGF
                     SKNLRQEMKPYNIKVTAVHPGAVMTDSWGDFDNSQQRIMEAADIAKLVYAASHLSPQA
                     CVEDIVVRPQLGDL"
     gene            1382..1969
                     /locus_tag="PK977_08045"
     CDS             1382..1969
                     /locus_tag="PK977_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRF18105.1"
                     /translation="MILSYFTKSISYTTISWDRYRNGYIKVDELRWKRMWLTLLDFKI
                     ADEPLAREMGIRFLDLLPTRKILFPYTFEILDYLSANNYELHLITNGFEKTQHSKLKY
                     SGLDKYFRHVITSEGSNSLKPHKEIFDYAFQKTGATPGQSIMIGDSMDVDILGAMNAG
                     IDQVHVNHLSNKLELINDQKPTYTVYSLKELEAIF"
     gene            complement(2050..>2673)
                     /locus_tag="PK977_08050"
     CDS             complement(2050..>2673)
                     /locus_tag="PK977_08050"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRF18106.1"
                     /translation="YSTIVFGVINLASFYFLSSSAPYISLLLFIVTLALLLFLISFFR
                     IPKRQHTIQENAIVAPADGKVVVIEEVQADEYFTDRRIQVSIFMSPLNVHVNRNPVSG
                     EVLYSQYHKGKYLVAWHPKSSTENERHSVVYRSTNGKEILVKQIAGALAKRIVNYLQP
                     GMQVKQTEEMGFIKLGSRVDLLLPLDAKINVKIGDKPQGGVTVIATW"
     assembly_gap    2674..2767
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2771..3697)
                     /locus_tag="PK977_08055"
     CDS             complement(2771..3697)
                     /locus_tag="PK977_08055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRF18107.1"
                     /translation="MAFNWSVFKTRALTAIVFVAVMLAGLLWNKWSFFILFSIIHFGC
                     WWEYLKLTEKIYSTSFQINAKLGFMLAGYGLMLWFCKDNYQIAGYSLSENFSMPVSGA
                     GFLLLIIGIFLTNPVHIHAFGMGLLGLLYISLSWGLMMDLTGFIDSFFYGSDIVFSAG
                     SLKIFFVIIPIVIIATIWINDTMAYIVGSLIGKTPLSKISPKKTWEGTIGGIILAIAV
                     VGLLAKFFIPLQYHLPGFVLEIANYHWFIIAAIAAIAGTFGDLLESKLKRMAGVKDSG
                     QIMPGHGGFMDRFDSLLLATPAVWLYVKLMML"
     gene            complement(3712..4665)
                     /locus_tag="PK977_08060"
     CDS             complement(3712..4665)
                     /locus_tag="PK977_08060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRF18108.1"
                     /translation="MYDSHSKGISYTAGFFMLIAFAVAGSILAGILSIPVWTNMTGLS
                     ITSMENAISDPANSNAVKVIQCISAIFGFFLPTLLTAKMMSRKPFRLLGFTGNSSFTE
                     AALVIGIMLTALFVSGGFAWLNEQIPVTDSLKKTFDKMEGDYNKQAEAILSLNSTVDY
                     IVAILVMAFLPALCEETLFRGGLQNFLTRATRNVWLSVIIVSILFSIAHFSYYGFLSR
                     VFLGVMLGLIYYFSGKLWLSILAHFVNNALAITVVYVYKLQGKPMAEAIKEGNDTAYW
                     GFIALPVVILLLVLFKKYARKPEQELPPADKNEELRNTPFY"
     gene            4705..5745
                     /gene="dusB"
                     /locus_tag="PK977_08065"
     CDS             4705..5745
                     /gene="dusB"
                     /locus_tag="PK977_08065"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRF18109.1"
                     /translation="MVKIGNIQLPDFPLLLAPMEDVSDPPFRAVCKDNGADLMYTEFI
                     SSEGLIRDAIKSRKKLDIFDYERPVGIQIFGGDEESLSLAAKIVEVTNPDLLDINFGC
                     PVKKVALKGAGAGVLRDIDLMVRLTDAVVKSTTLPVTVKTRLGWDDHTKNIEEVAERL
                     QDVGIKALAIHGRTRTQMYKGEADWTLIGKVKNNPRISIPIFGNGDIDSPEKALEYKN
                     RYGVDGIMIGRAAIGYPWIFNEIKHFIKTGEHLPAPTIEDRVAVCKKHLHKSVEWKNP
                     VVGVNEMRRHYANYLKGLPNIKEYRNKLVTLKSVEEINAVLNEIEKVYTGYTFEKTPI
                     ELVNYHEKCPVN"
     gene            5774..6691
                     /locus_tag="PK977_08070"
     CDS             5774..6691
                     /locus_tag="PK977_08070"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF18110.1"
                     /translation="MQTILGASGIIGTELAKALPAYSSSIRLVSRTPKKVNETDELVA
                     ADLLNAAQTETAVQGSEIVYLAAGLVYKTTVWREQWPIVMRNVIDACKKQGAKLVFFD
                     NIYMYGPVTGVITEETPFNPSSEKGKIRAAIANMLLNEVKSGNLTAMICRAPEFYGPG
                     KTLSVVNAMVFDNIRKGKKAQWLITDQSKRTFIYTPDAGKATALLANSPGTWNQTWHL
                     PCDESFPTGKEFMQLINEVTGQEINHSVLSRFMINMAGLFVPSIREIKELLYQFEQDY
                     IFSSDKFKKAFPSFSITSYREGIAAILNR"
     gene            complement(6681..6932)
                     /locus_tag="PK977_08075"
     CDS             complement(6681..6932)
                     /locus_tag="PK977_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18111.1"
                     /translation="MNYNDIIGTLGVSLILLAYFLNVFGWLSNKNKLFYLLNIVGAGL
                     ACYASWLIRYWPFVILEGVWCLVSVIGLLKSTLNKKPTG"
     gene            complement(6939..7619)
                     /locus_tag="PK977_08080"
     CDS             complement(6939..7619)
                     /locus_tag="PK977_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRF18112.1"
                     /translation="MRKLKRLAIVILLLIAVFVVYVEIVNRNSKNMTYRQKVLKAVYP
                     AWMWITKLTGKNTKKMNNESAKTPFQSFYDLSATQIDGKEIKFADFKGKKLLLVNTAS
                     DCGYTNQYADLQKLYEDFSNKLVVIGFPANDFKEQEKGTDKEIAQFCQKNYGVTFPIM
                     KKSVVIKSEQQNPVYQWLTDSSKNGWCNQQPSWNFSKYLLDENGVLTNYFDPSVSPLS
                     KEITDKLR"
     gene            complement(7616..>8390)
                     /locus_tag="PK977_08085"
     CDS             complement(7616..>8390)
                     /locus_tag="PK977_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF18113.1"
                     /translation="AAERLVPVTLELGGKSPCVVERDANIKVAARRIAVTKFSNAGQM
                     CVAPDYVLVHSSVKDKLLSELKATLPKFFSEKPEESYNYGKIINERQFNRIAGYLSSG
                     SIEFGGRTDKEKLFIEPTILTNIALDAPVMNDEIFGPVLPVISFETMEEAKSIIDRHP
                     NPLSFYIYTSSSRKEEEWLQAIPAGGACVNNSSWHLTNHHLPFGGRGFSGMGCYHGKY
                     SFETFSHKKAVMKTPTWFDPAIKYPPFKGKLRLFKWVIK"
     assembly_gap    8391..8482
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8483..9002)
                     /locus_tag="PK977_08090"
     CDS             complement(<8483..9002)
                     /locus_tag="PK977_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF18114.1"
                     /translation="MPDIASLEKMHQYFNRGETKPYSFRKEQLKKLKSSIINHEEDLY
                     NALYADLKKSKEETWVTETGMVISELNAAIKDLRNWMEPERVSTNLLNLPSGSRVMKE
                     PLGVVLIIGPWNYPFQLLINPLIGALAAGNCVVLKPSEFAPATDAVMKKIIEDVFPPE
                     YILYVQGEGQTVI"
     assembly_gap    9052..9288
                     /estimated_length=237
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9289..9486
                     /locus_tag="PK977_08095"
     CDS             <9289..9486
                     /locus_tag="PK977_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRF18115.1"
                     /translation="PCFMCAGAAYWSKIGKVVYGADDEKNGYKKTAGTNWPFHPKTEL
                     IRGVMKEECAQLMKDFFKSKR"
     assembly_gap    9650..9662
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9964..10046
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10047..>10360
                     /locus_tag="PK977_08100"
     CDS             <10047..>10360
                     /locus_tag="PK977_08100"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRF18116.1"
                     /translation="TPHENKSLEELVAAAGSISPAVRNNGGGHWNHTFFWESLGPNAG
                     GTPTGKLADAINEAFGSFDAFKEKFANAGMTRFGSGWAWLIVIEGKLEITSTPNQDNP
                     LM"
BASE COUNT         2959 a         2157 c         2061 g         2664 t
ORIGIN      
        1 tgggtgatca ctttcaccca tacatcactg ctttgcagta attgcccgtc agcaacaatg
       61 gtagtaccgg gaatcataaa ccggcccggc cgggtagctt ccagggtgta cacaaaattc
      121 cgcagtggtt ttgagccatg aacggatggt acagagccgg tatagagatt gggccccgcc
      181 acatagcgaa agtttttaaa aatcggcggc ttcaggttac tggcattatc agcatcctcc
      241 agtatgtact gcacctgaaa agattcgccg gccaccaacg gctgctgagg tactacggtt
      301 ttaaagctta cctgtgcctg caataaaacc gaacataaca aaaccgctat agagaaaaaa
      361 aaccgcataa ttacccggct aaagtaaaga tgcttttgaa aaagggaatg taaaaattat
      421 gacaggacta tgaaagagat taacggttga ttttaatagt aaataaaaaa taagttccaa
      481 aagattgatt tctacaaatc ccccaactgc ggtcgcacca caatatcctc cacgcaggcc
      541 tgcggtgata agtgtgaagc agcatacact aatttggcaa tatctgctgc ttccatgatg
      601 cgttgctggc tgttgtcaaa atcgccccat gaatccgtca ttaccgctcc ggggtgcaca
      661 gcagttactt taatattata cggcttcatc tcttgccgca ggtttttgct gaatccatac
      721 agggcaaatt tgctgatgct gtacgcaccg ccattagcat aggcatccag tgaggcaatg
      781 gaacacatgt taaaaatatg gcctgatctt ttttccatca tccatggcag aacggaccgg
      841 gttacatgat aagcgctgtg caggtttacg gccagctgac tttccagtgt tccctccggt
      901 tcattgtgca cactgccagg ttcaaaaaga cccgcattgt ttaccaatac atccggcaca
      961 caatattcca ggcaccaggc gccaaaagcc ttggcctctt ccttacggct gagatcaaaa
     1021 ggttttgctt tgatgatagc agcaggatat ttggtcatca actcttccat ggttttatac
     1081 aaagccactt cgcctcttga acagaggaac aaatgatggc catatgcggc aaaaatttct
     1141 gctatggctt ttccgattcc ttttgatgcg ccggtgataa ctacattcat aatccaaagt
     1201 tagaaatacg aagtacgaac atttttaccg tacttcttac tttgtacttc gtactttgca
     1261 ggtatgagat accgacacct tttttttgat cttgaccata ccctttggga cttcgaagcc
     1321 aacagccgtc aaaccctgga tgaattgtac cattcactcg gcctggccga aagaggtatt
     1381 catgattttg agttatttca caaaaagtat ctcgtacaca acgataagct gggaccgcta
     1441 ccgcaacggc tatatcaaag tagatgaact gcgctggaag agaatgtggc tgaccttact
     1501 ggatttcaaa attgcagacg aaccactggc cagggaaatg gggatccgct tcctcgactt
     1561 gctgcctacc cggaaaatac tttttcctta tacttttgaa atactggatt acctgtcggc
     1621 caacaattat gagctgcacc tgatcaccaa tggttttgaa aaaacacagc acagcaagct
     1681 gaaatattct ggactcgata aatatttccg gcatgtgatc acatcagaag gcagcaacag
     1741 cctgaaaccg cataaggaga tctttgatta tgcttttcag aaaacgggag ctaccccggg
     1801 acaaagcatt atgatcggcg acagcatgga tgtagatatt ttaggagcta tgaatgcagg
     1861 tatagaccag gtacatgtaa accacctttc gaataagctt gaattgatca atgatcaaaa
     1921 gccgacttat acggtttatt cgttgaagga gttagaagca atcttttagt gtcccgtaaa
     1981 gacataaaag acgctgagta tactaggtaa attaaactat ttgagttatt ggtggcttag
     2041 tgggagtcct taccaggttg caatcaccgt cacaccgccc tgcggcttgt caccgatctt
     2101 tacattgatc tttgcatcca gcggcagcag caaatctacc cttgagccta atttgataaa
     2161 gcccatttct tcggtttgct tcacctgcat accgggttgc aggtaattaa cgatgcgttt
     2221 ggcgagggcc ccggcaattt gctttaccag tatttctttt ccgttggtgc tgcggtacac
     2281 taccgaatgc ctttcgtttt ctgttgatga cttgggatgc caggctacca ggtatttacc
     2341 tttgtggtac tggctgtaca atacttcacc gctgacggga ttgcggttca catgcacatt
     2401 caacggactc atgaaaatag aaacctggat gcgccggtct gtaaagtatt cgtcggcctg
     2461 cacttcttct atcaccacta ctttaccatc tgcaggagca acgatggcat tttcctgaat
     2521 ggtatgctgc cgtttaggaa ttctgaaaaa tgaaatgaga aacaataata atgctaacgt
     2581 gacaataaaa agcagaaggc tgatatacgg ggctgaagaa cttaaaaaat aaaacgaagc
     2641 caggttgata acaccaaaca caatggtgct gtannnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnttc ttataacatc atcaacttta catacaacca aacagcagga gtggcaagca
     2821 gcaacgaatc aaacctgtcc ataaaaccac catgacccgg cataatctgg ccgctgtctt
     2881 ttacaccagc cattcgtttt aatttggatt cgagcagatc gccgaatgtt ccggcaatgg
     2941 cggcgatggc agcaataata aaccaatggt aattggcgat ttccaaaaca aatcccggca
     3001 aatgatactg aaggggaata aaaaatttag ccagtaaccc caccactgca attgccagga
     3061 taatccctcc gatggttccc tcccaggttt tcttgggtga tatttttgac agtggtgttt
     3121 tgccaatcag ggaaccaaca atataggcca ttgtatcatt gatccagatg gtggcaataa
     3181 tcacaatcgg aatgatgaca aagaaaattt ttaaactgcc ggccgaaaaa acaatatcac
     3241 ttccataaaa gaaagaatca ataaatccgg tcaaatccat catcaatccc cagcttaatg
     3301 agatatagag caggcctagt aatcccatcc cgaatgcatg tatatgcacc gggttggtca
     3361 gaaatatgcc aatgatcaat aacaggaatc cagcacctga aacgggcatg gaaaaatttt
     3421 cggaaaggct gtaaccggca atttgataat tatccttaca aaaccacagc atcagtccat
     3481 aaccggccag catgaagccg agtttggcat taatttgaaa ggaagtgctg tatatttttt
     3541 ccgtcagctt aaggtactcc caccagcagc caaaatgaat gatggaaaac aggatgaaaa
     3601 agctccattt attccaaagc agcccagcca gcatgacggc gacaaataca atagctgtaa
     3661 gagcccgggt tttaaaaact gaccagttaa atgccatagc aggatgatac tttaataaaa
     3721 tggtgtattt cggagctctt catttttatc agccgggggc aactcctgtt cgggttttct
     3781 cgcatacttt ttaaacaata ccaacaaaag tattactaca ggcaaggcga taaatcccca
     3841 ataagccgtg tcatttcctt ccttaatggc ttctgccatt ggttttccct gtagtttata
     3901 tacatatacc actgttatgg ccagtgcatt attgacaaaa tgcgccagta tactcaacca
     3961 tagtttgccg gagaaatagt agatcaaccc cagcattact cccaggaaga cccttgataa
     4021 aaatccatag taagaaaaat gggcaatgct aaaaagtata ctgacaataa taacagacag
     4081 ccaaacattg cgggtggcac gggtcagaaa gttttgtaac ccgccgcgga ataaggtttc
     4141 ctcgcaaagt gccggcaaaa aagccatgac gaggatagcg actatataat caacggtgct
     4201 gttgaggctc aatatcgctt ctgcttgttt gttataatca ccttccattt tatcaaaagt
     4261 cttcttcagc gaatcagtaa caggtatttg ttcatttagc caggcaaaac cgccggaaac
     4321 aaacagagcg gtgagcatga taccaattac caatgctgct tccgtaaaac tgctgttgcc
     4381 ggtgaaaccc agtaaccgaa atggcttgcg gctcatcatc ttggcagtaa gcagtgtggg
     4441 caggaagaaa ccaaatatcg ccgagatgca ttgaatcact ttcaccgcat tactgttggc
     4501 cggatcgctg atggcatttt ccatgctggt gatggaaaga cccgtcatat tggtccatac
     4561 cggtatactt agtatgcctg caaggatgga accggccact gcaaaagcta tcagcataaa
     4621 aaaacctgct gtataggata tcccttttga atgactgtcg tacataaact ctggtgatta
     4681 aactgtaaat ttgccgtttc ttaaatggta aagataggca acatacaact tcctgatttc
     4741 ccgctgttgc tggcgccaat ggaggatgtc agtgatccgc cgttccgtgc cgtttgcaag
     4801 gacaacgggg ctgatttaat gtacaccgag ttcatcagca gcgaaggatt gatcagggat
     4861 gctatcaaaa gccgtaagaa acttgacata tttgactatg aacgtccggt aggcatacag
     4921 atctttggcg gagatgaaga aagcctttcg ctggctgcca aaatcgtgga ggtaacgaat
     4981 cccgatttac tcgatataaa ttttggctgc ccggtaaaga aagtagcatt gaaaggagcc
     5041 ggtgccggtg tattaaggga tatcgattta atggtaagac ttacagacgc cgttgtaaaa
     5101 tctaccacat tgccggttac agtaaaaaca aggctgggct gggatgatca tacgaagaac
     5161 attgaagaag ttgccgaacg tttgcaggat gtgggcatca aagcactagc tattcacggc
     5221 cgtacaagaa cgcagatgta taaaggcgaa gccgactgga cattaatcgg taaagtaaag
     5281 aacaatcccc gcatcagcat tcccattttt ggcaacggcg atattgattc tcctgaaaaa
     5341 gcattggaat acaaaaaccg gtatggcgtt gatggcatta tgattggccg ggcggctatc
     5401 ggctatccat ggatattcaa tgagatcaaa catttcatca aaacaggaga acatttaccg
     5461 gctcctacga ttgaagacag ggtggctgta tgtaaaaaac acttacataa atctgtagaa
     5521 tggaaaaacc cggtagtagg agtaaatgaa atgagaagac attatgccaa ctacctgaag
     5581 ggcctgccga atatcaaaga ataccgcaac aaactggtaa cccttaaatc agttgaagaa
     5641 attaatgccg ttctgaacga aattgaaaag gtgtacacag gttatacttt tgaaaaaact
     5701 cccatcgaac tggtgaacta tcatgaaaag tgcccggtta attaaggctg gtgtaacttc
     5761 acacaaaaat tttatgcaaa ctatactcgg agccagtggt ataattggca ctgaactggc
     5821 aaaagcctta cctgcttatt cttcttctat tcgtttggtc agccgtactc caaaaaaagt
     5881 aaatgaaacc gatgaacttg ttgctgccga tttgctgaat gcagcacaaa cggaaactgc
     5941 agtacaaggt tctgaaattg tttacctcgc tgcaggactg gtttacaaaa caacggtgtg
     6001 gcgggaacaa tggcctattg taatgcgtaa tgtgattgat gcctgcaaaa aacagggagc
     6061 caaacttgtt ttctttgaca atatttatat gtatgggccg gtcacggggg ttataacaga
     6121 agaaacacca tttaatccct ccagtgaaaa gggaaaaatc agggctgcta ttgcaaatat
     6181 gctgctgaat gaggtaaaaa gcgggaatct taccgccatg atctgccggg cacctgaatt
     6241 ttatggtccg ggtaaaacat tgagtgtagt caatgccatg gtatttgata atatccggaa
     6301 aggcaagaaa gcgcaatggt tgattacgga ccaatccaaa cgaactttta tttatacccc
     6361 agatgccggt aaagccactg cattgctggc caattctccc ggtacatgga accagacctg
     6421 gcatttacct tgcgatgaaa gttttcccac cggaaaagaa tttatgcaat taattaacga
     6481 agtaacaggc caagaaatta atcatagcgt cctttcccgt tttatgataa atatggcggg
     6541 attatttgta cctagtatca gggaaataaa ggaattgctg taccagtttg aacaggatta
     6601 tattttcagc agtgacaaat ttaaaaaggc cttcccttct ttcagcatca ccagctacag
     6661 ggaaggtatt gcggcaatcc ttaaccggta ggcttcttat tcagtgttga tttcaataaa
     6721 ccgattactg aaacaaggca ccaaacccct tccagtatta caaaaggcca gtaccgtatc
     6781 aaccaggatg cataacaggc taatcctgca ccaacaatat taagcaggta gaataattta
     6841 ttcttattgc tgagccagcc aaaaacattg agaaaatagg caagcaaaat gaggcttacg
     6901 ccaagagtac caatgatatc gttatagttc atcattgctt acctgagctt atcagttatc
     6961 tctttactta gaggagaaac tgaaggatcg aaataattgg tgagcactcc gttttcatcc
     7021 aacaggtact tggaaaaatt ccagctgggt tgctggttac accatccgtt tttggatgaa
     7081 tctgtgagcc actgatacac cgggttttgt tgttctgatt ttatcaccac tgattttttc
     7141 ataatgggga atgtaacccc atagtttttt tggcagaact gtgctatttc tttatctgtt
     7201 cccttttcct gttccttgaa atcatttgcg ggaaaaccga ttaccaccag tttattactg
     7261 aaatcttcat ataatttttg caggtcagca tattgattag tgtagccgca atcagaagcc
     7321 gtattcacca gcaacaactt tttcccttta aagtcagcaa attttatttc tttcccatcg
     7381 atttgagtag cagacaaatc ataaaatgat tgaaaggggg ttttagccga ttcgttattc
     7441 atttttttgg tattttttcc ggtaagctta gtgatccaca tccaggcagg gtaaacggct
     7501 tttaatactt tctggcggta cgtcatattc tttgaattac ggttgacaat ttctacatac
     7561 accacaaata cagcgatgag cagtaaaata acaattgcca gcctttttag ttttctcatt
     7621 ttatcaccca tttaaataat ctcagttttc ccttaaatgg cggatacttt atagcggggt
     7681 caaaccaggt gggtgttttc attacagctt ttttatggct gaaggtttcg aatgaatatt
     7741 tgccgtgata acaacccatc ccgctaaatc ccctgccgcc gaaaggcagg tgatgattgg
     7801 tcaggtgcca gctggagttg ttgacacagg caccgccagc aggtatcgcc tgcaaccatt
     7861 cttcttcttt cctggaactg gatgtataaa tataaaacga taagggattg ggatgccggt
     7921 caataataga ctttgcctct tccattgtct caaaggagat aacaggaagt acagggccga
     7981 atatttcatc attcatcact ggtgcatcaa gagcaatatt tgtcagtatg gtgggttcaa
     8041 taaacagttt ttctttatct gttctgccgc caaattcgat ggaaccgctt gacaagtatc
     8101 cggcaatcct gttaaactgt ctctcattaa tgatctttcc gtagttataa ctttcttccg
     8161 gcttttcact gaaaaatttc ggcagggttg ctttcaactc actcagtaat ttatctttta
     8221 ccgaactatg caccagcaca taatccggcg ccacacacat ttgtccggca ttggaaaatt
     8281 tggtgacagc aatacggcgg gccgctactt ttatatttgc atccctttcc accacacaag
     8341 ggcttttacc acccagctct aatgtaaccg gtactaatct ttccgctgcc nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nngaataacg gtttgtcctt ctccctgcac atataaaata
     8521 tattccggcg gaaaaacatc ttcaatgatc tttttcatga cagcatctgt agccggagca
     8581 aattcactgg gttttaacac cacacaattg ccggcggcta atgcccctat cagcggattg
     8641 ataagcaact gaaatggata attccacgga ccgatgatca atactacacc caatggctct
     8701 ttcatcaccc tgctgcccga tggaaggttg agtaaattag tggatactct ttccggctcc
     8761 atccagttgc gcaggtcttt aatagcagca ttcagctcag aaatcaccat acctgtttct
     8821 gtcacccagg tttcttcttt gctttttttc agatcagcat acaaagcatt atacagatct
     8881 tcttcatgat tgataatgga ggattttagt ttctttagtt gttctttgcg aaacgaataa
     8941 ggctttgttt cccctctgtt gaaatattgg tgcatttttt ccaggctggc tatatctggc
     9001 attagttcta attttataac aaggtacaca aatatgacgg acgaatattt cnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ttgttttatg
     9301 tgtgctggtg ctgcctactg gagcaagatc ggcaaagtgg tttacggtgc tgatgatgaa
     9361 aagaacggct acaaaaaaac agccggcacc aactggccct ttcaccccaa aacagaattg
     9421 ataagagggg tgatgaaaga ggaatgtgcg cagctgatga aagatttctt taaatctaaa
     9481 agataatata tggcaaaagc ggctacaaaa aagaaggtga ctactacaca tccccgcacc
     9541 ggcagccagt ttaccattgc cgctgaaatc tatgaaccat ttaaagcagc gattttacaa
     9601 agtttgaagg ggagcaaggg caaaaccttt actgaactga cagacaatgn nnnnnnnnnn
     9661 nncatcaaaa agaaaatgcc cggcttcaag aaatctatac cctggtacac tatatccgtc
     9721 cgactcgacc ttgaatcaag aggcatagtg gaaactttta ccgaaaaagg caaaaagctt
     9781 aaccgtctaa cccaatagtt ttcgagatag gaaacaattc aaacaggagc ctgatttatt
     9841 aaactccaac tgcctacttt tgttatccaa ttaaccaatt tacattctaa catttaaaat
     9901 actttactat gtcatttaca ctagcacccc ttccttatgc acatgatgcg ctggaaccgc
     9961 atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnacac cacatgaaaa taaatctctg gaagaactgg
    10081 tagcagctgc aggatcaatt tcaccagctg taagaaacaa tggcggcgga cactggaacc
    10141 acactttctt ctgggaaagc cttggcccta atgccggcgg cacaccgacc ggaaaattag
    10201 ctgatgccat caatgaagct tttggttctt ttgatgcatt caaagaaaaa tttgccaatg
    10261 ccggcatgac aaggtttggc agcggatggg catggctgat cgttatagag ggtaagttag
    10321 aaatcacttc tacccctaac caggataatc ctttgatgga
//
LOCUS       DAORLS010000085        10360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3454_length_10360_cov_1.831053, whole genome shotgun sequence.
ACCESSION   DAORLS010000085 DAORLS010000000
VERSION     DAORLS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_10360_cov_1.831053"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK977_08105"
     CDS             <1..456
                     /locus_tag="PK977_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRF18117.1"
                     /translation="KLSVNLSKRTNITEDDIENYIGVSKDYNVFELQAALAAKDLARS
                     IRIIQYFEANPKAGPIQLVLPSIYSFFSKVFMIFGTGTTDEKTIATAIGVNPYFMKEY
                     MQAARLYTYPGVERALLLLHSYNLKSIGVGSSTTEDASLMKEMVVKMMA"
     assembly_gap    728..747
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <748..1345
                     /locus_tag="PK977_08110"
     CDS             <748..1345
                     /locus_tag="PK977_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18118.1"
                     /translation="AVDLEKAGDMAKDTVISMAQGADSIPNLTPEEKEILRKGSLRVN
                     MNIKDEKFLTSLSLPFSKPSDITMISKLSGKAMSEAMKEQMADGSPVGGNNEIPETSS
                     FDDYYKYEFSEGELTRKLDKDKYAGVESDEFLKGMKEASAMGLSMKATYVINLPRPAQ
                     KAEGKGVKLSDDKKKVTITADLDDFFADPSKLEYKIKY"
     gene            complement(1407..>2170)
                     /gene="ribF"
                     /locus_tag="PK977_08115"
     CDS             complement(1407..>2170)
                     /gene="ribF"
                     /locus_tag="PK977_08115"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRF18119.1"
                     /translation="PRKVVSSAILGIRLINTLEERIELLEGLGIDHLVIVPFTDAFAN
                     QPAEDYVKNFLIDKFHPSTIIIGYDHRFGRDRLGDYRLLEKMANIFDYKLKEIPKHIL
                     ENISISSTNIREAILHSDITTANRLLGYEFFFSGEVVHGDKLGRKLGYPTANLRIQNE
                     EKIIPGNGIYAVYAKSEETAQTLKGMMSIGFRPTVDGKKRVVEVNLFDFDKEIYGQTL
                     KVYVKSFLREEVKYDSLEALVKQIDQDKINSLKVL"
     assembly_gap    2171..2191
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2192..2267)
                     /locus_tag="PK977_08120"
     CDS             complement(<2192..2267)
                     /locus_tag="PK977_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18120.1"
                     /translation="MGHRQIIDKLKSEAKAINGETVIIT"
     gene            2430..>3165
                     /locus_tag="PK977_08125"
     CDS             2430..>3165
                     /locus_tag="PK977_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRF18121.1"
                     /translation="MSLYSKRGVSAQKEEVHEATKKLDKGLFPKAFCKIYPDVLCGDD
                     AWVNVMHADGAGTKSILAYLYWKETGDISVWKGIAQDAIVMNLDDLLCVGIFDNILFS
                     STIDRNKNVIPAEVLEAVINGTQDFFNTMKDFGVNISFMGGETADVGDVVRTIAVNGT
                     MTARWPKDKLITNEKIKPGNVIVGLAGFGKANYESSYNSGIASNGLTSARHDVLHKTY
                     GARFPESYDTSLDESVVYIGQNRVTDM"
     assembly_gap    3166..3252
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3253..>3547
                     /locus_tag="PK977_08130"
     CDS             <3253..>3547
                     /locus_tag="PK977_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRF18122.1"
                     /translation="EDHFETINGLVHCSGGGQTKCLKYVPENVRIVKDNLFAPPVIFR
                     LIQEASKADDREMYQVFNMGTRLEIYTNEGNADSIISVSKSFGVDAQVIGRVEK"
     assembly_gap    3548..3749
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3750..>3957)
                     /locus_tag="PK977_08135"
     CDS             complement(<3750..>3957)
                     /locus_tag="PK977_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18123.1"
                     /translation="VEIEARFPGGDAIWRRYLERNLDGSTPTKNGAPEGTYTVVVQFI
                     VDKEGNISDVKALTNHGYGMEAEVV"
     assembly_gap    3958..4139
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4273..5238
                     /locus_tag="PK977_08140"
     CDS             4273..5238
                     /locus_tag="PK977_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRF18124.1"
                     /translation="MQSFELLSRKFALHFEQQHFPAEPASLYQPNEYFLKLGGKRIRP
                     VLCLMGNELFDDIKTDAWEVGSAIELFHNFTLIHDDIMDKAPLRRGMETIHAKYGENV
                     ALLAGDVMLVKAYQQLEKVQLEFQHPILHLFNKTAAEVCEGQQWDMDFEKKETVKLDD
                     YLRMIELKTSVLLAASLKMGAILGGAGERNQNLLYEFGKKLGIAFQVQDDYLDAFGDP
                     QKFGKQVGGDILANKKTFLLIHAFETVNDPQKDELNELLAANAPDKVEKILKIFRDCK
                     VDEWALELKNKYLDEAFSHLEDIAVVSKRKQPLKELGQFLVKREH"
     gene            5308..6690
                     /locus_tag="PK977_08145"
                     /pseudo
     CDS             5308..6690
                     /locus_tag="PK977_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6772..6948
                     /locus_tag="PK977_08150"
     CDS             6772..6948
                     /locus_tag="PK977_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HRF18125.1"
                     /translation="MAFVKRFSLIPILGMMSCFYLMAQETHKVWMRFLIWLAIGLCVY
                     FLYSYRNSKLAKGK"
     gene            7013..>7434
                     /locus_tag="PK977_08155"
     CDS             7013..>7434
                     /locus_tag="PK977_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18126.1"
                     /translation="MKYQIGDIVLILHSNEEAEVVDIINDKMLMVDVKGVSFPVYKDQ
                     VDFPYFKRFTEKKPVSAKKERTYIDDVRKEKRQEQNRVEDGVWLTFLPVMDIDEFGDV
                     IVEELKLHLVNHTSLPYNFIYKLHFFGKPDFELKNTVQP"
     assembly_gap    7435..7518
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7519..8041
                     /locus_tag="PK977_08160"
     CDS             <7519..8041
                     /locus_tag="PK977_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRF18127.1"
                     /translation="LLIPDKKKAGHFEASVKLKPKQLFAKIEELKQKNLATFSQLLFE
                     KYPDKAVEDVVEMGRMAAKGYKVYDASKARQHLESPRSVIDLHIEKLTDDWKHMSNYE
                     ILSLQLKTFEKYYHLSVIHHQPSLIVIHGVGEGVLRDEIHDILRLKKEVKSFVNQFHP
                     AYGYGATEIFFQY"
     gene            8066..8444
                     /gene="rnpB"
                     /locus_tag="PK977_08165"
     ncRNA           8066..8444
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK977_08165"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(8445..9344)
                     /locus_tag="PK977_08170"
     CDS             complement(8445..9344)
                     /locus_tag="PK977_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18128.1"
                     /translation="MKEIIYYIKSYYKETDRTILLLISLFTAFLVFCNYYFGLDTFIR
                     KKNLFVVKLFCWYVVFAAAFIVPYLFYGFARKKNHLKNKNFYILLVVTPLIFAWKISV
                     SLVISFSENQTNNHFWNQVGYWPALAIVIFLLLYFIWKLYHEDQPLYGLRTGNMAWKP
                     YLLMLLIMFPLITLAATQNDFLSVYPKLNSVSSEAIWWQKLLFELSYGSDFFTIEFFF
                     RGFLVLGFAKWVGKDAILPMDCFYCTIHFGKPLGECISSYFGGLLLGIVVYNTRSIWG
                     GLIVHLGIAWMMEMTGFIGNQLK"
     gene            9446..>10360
                     /locus_tag="PK977_08175"
     CDS             9446..>10360
                     /locus_tag="PK977_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18129.1"
                     /translation="MKTITLNRMMIAMFAVLLSIFVISCQKESSLTEPTTLTEEEAAT
                     YSEESTQAEASFDDAEDIGFTAAEEEGNAGGFGLEGRSASEQGRVFLPNFAELRGRIG
                     DCATITVTPNDSTYPKTVTIDFGDSCRGRDGKVRSGKIVLHFTGPIRRPGSVVTMTFV
                     NYYVNRVHIEGTKIFKNLSEPPMLHKWSVEVLDGKVTFPNGRGYTYDGIKTVKQIAGM
                     LTNVVRDDVYKVTGRSKTVFRNGTTVNINTEDPLIKKVACHWISEGTLKIKINSRILF
                     LDFGFPNNGGCDNKALLTWNNGNNQRIII"
BASE COUNT         2977 a         1807 c         2117 g         2863 t
ORIGIN      
        1 aaactatcag taaacctgag taaaagaaca aatattacag aagatgatat tgagaactat
       61 ataggggtaa gtaaggatta taatgtattt gagcttcaag ccgcactggc ggcaaaagat
      121 ctggcgagaa gtattcgcat tatccagtat tttgaagcta accccaaggc cggcccgatt
      181 caactggtac ttccctctat ttacagtttt ttcagtaagg tatttatgat atttggtaca
      241 ggtactactg atgaaaaaac cattgctacg gctattggag tgaatccata ctttatgaag
      301 gaatatatgc aggcagcccg gctttataca tacccaggtg tagaaagagc tcttctctta
      361 ctgcatagct acaaccttaa aagtattgga gtgggcagtt cgactactga agacgcaagt
      421 ctgatgaagg aaatggtcgt aaaaatgatg gcctgaacaa tttgtgacct accaccaaaa
      481 gccctttctt tgtgcaactt ttcaaattta acgtacatct tcttgaacaa atactaaaaa
      541 catttgaatt caataagcaa taaaaaacaa atcatgaaaa aattactctt ctcctttttt
      601 gcttttactt ttttacttgc cggctgcctt gaaacaacac aggaaattac catcaatgag
      661 gacggaagcg gcacggttgt aaacaccaat gattttagtg ctctcattgg tctggccaaa
      721 caaatggnnn nnnnnnnnnn nnnnnnnagc tgtagatctt gaaaaagcag gtgacatggc
      781 caaggatacc gttatttcca tggcacaggg tgctgatagc attcctaacc tgactcctga
      841 agagaaggaa atactgcgca aaggatccct gagagtaaac atgaatatta aggatgaaaa
      901 attcctaacc agcctctctc ttccttttag caagccctca gacattacaa tgatcagcaa
      961 gctttctggt aaagccatgt ctgaagcgat gaaggaacaa atggccgatg gctcaccggt
     1021 gggtggtaat aatgaaatac ctgaaacaag cagtttcgat gattattata aatatgagtt
     1081 ctccgaaggt gaacttaccc gtaaattgga taaggataaa tatgcaggag tagagagtga
     1141 tgagtttctg aaggggatga aggaagcatc ggctatgggt ctttccatga aagcaaccta
     1201 tgtgattaac ctgccaagac cggctcaaaa agctgaaggg aaaggtgtga aattatcgga
     1261 tgacaaaaag aaggtaacaa ttactgctga ccttgatgat ttttttgcag atccttcaaa
     1321 actggaatat aaaatcaaat actagtatag aaaaaatata aagtaagaaa aggcaactct
     1381 gaaaggttgc cttttcttat atccttttac agtactttca aactgttaat cttgtcctgg
     1441 tcaatttgct ttaccagtgc ttcgaggcta tcatacttta cttcttctct taaaaacgat
     1501 ttaacatata ccttcagcgt ttgcccgtaa atttctttat caaaatcaaa caggttaacc
     1561 tcaactactc tttttttacc atcaaccgta gggcggaacc cgatactcat catgcctttt
     1621 agcgtttgtg cagtttcttc ggattttgcg tatacagcat aaatgccatt tccaggtatt
     1681 attttttcct cattttgaat tctcagatta gccgttggat atcccagttt acgtcccaat
     1741 ttatcaccat gcactacctc tccggaaaaa aagaactcat accccagtaa cctgttggct
     1801 gtagtgatat cgctgtgcag aatggcctct ctgatattgg tggaacttat ggaaatgttc
     1861 tccaggatat gtttgggaat ctctttcaat ttataatcaa agatgttggc cattttttcc
     1921 agcaagcgat aatctcccag ccggtccctg ccaaaccggt gatcataacc tataataata
     1981 gtgctgggat gaaacttgtc aataaggaag ttttttacat aatcctctgc aggctggttg
     2041 gcaaaagcat ctgtaaaggg aacaattaca aggtggtcga tgcccaaccc ttccagcagt
     2101 tcaattcttt cttccagggt attaattaaa cggataccta agatagcaga cgatacaact
     2161 tttctgggat nnnnnnnnnn nnnnnnnnnn naggtgataa taacggtttc gccattaatg
     2221 gcttttgctt ctgacttcag tttatcgatt atctgcctgt gtcccatatg tacaccgtca
     2281 aaagtgccga tggtaataac tgcgttttga aaaagcggaa gttgctctat atcacgatgt
     2341 attttcataa ttgagtgggc aaaactactt gataaattgg taactgcgta ttgggatttg
     2401 aacattagat ttgccgggta tttacaatta tgagtttata tagtaaaaga ggggtttctg
     2461 cacagaaaga agaggtgcat gaggctacca aaaagttaga caaaggcctt ttccccaaag
     2521 ctttttgtaa aatttatccc gatgttttat gtggagatga tgcatgggtg aatgtaatgc
     2581 atgctgatgg ggctggcacc aagagtattc tggcttacct gtattggaaa gaaactggtg
     2641 atatttcagt atggaaaggc attgcacagg atgctattgt gatgaacctg gatgaccttc
     2701 tttgcgtggg catttttgac aacatccttt tttcttcaac tatagacagg aataagaatg
     2761 ttataccggc agaggtattg gaagcagtga tcaacggtac gcaggatttt ttcaatacca
     2821 tgaaggattt tggtgtaaac atcagcttta tgggcggcga aactgctgat gtaggtgatg
     2881 tggtacgtac tattgctgtt aacggaacaa tgacggcaag atggccgaaa gataaattga
     2941 tcaccaatga gaaaattaag cccggcaatg tgattgtggg cctggcaggc tttggtaagg
     3001 ctaattacga atcttcctat aacagtggta tcgccagtaa cgggcttacc agtgcaaggc
     3061 atgatgtatt acataaaaca tatggagccc ggtttccaga gagctatgac acctcgctgg
     3121 acgaatcagt agtttatatt ggtcagaaca gagtgacaga tatggnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nntgaagatc attttgaaac cattaacggg ttggttcatt gcagcggtgg
     3301 cggacaaacc aaatgcctga aatatgtgcc ggagaatgtt cgtatagtaa aagataattt
     3361 atttgctccg ccggtaattt tcagattgat acaagaagcc agcaaagctg atgaccggga
     3421 aatgtaccag gtatttaata tgggtaccag gctggaaatt tataccaatg aaggaaacgc
     3481 tgactcgatt atcagcgtct caaaatcttt tggtgtggat gcacaggtaa ttggaagggt
     3541 ggaaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acaacctcag cttccatgcc ataaccatga
     3781 ttggtcaatg cttttacatc tgaaatattt ccctctttat ctacaataaa ctgaactact
     3841 actgtgtaag tgccctcggg tgcaccattt tttgtgggtg ttgagccatc aaggtttctt
     3901 tctaaatacc ttctccaaat ggcatcacca cctggaaatc tggcttcaat ctccaccnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4141 acaactatgc ttaaaaagtg gttcattaaa ttgtaattgg ttatttatgg cagtaaatat
     4201 ctgcaaaatt gatttacagg cattcttttt cttactttgt acacaacatt ttttcggaat
     4261 atatttacac ctatgcaatc cttcgaactg ctatccagga aatttgctct tcattttgag
     4321 cagcagcatt tccctgctga gcctgccagc ctgtatcaac ctaatgaata ttttttaaag
     4381 ttaggtggca aacgaatccg cccggtgctg tgtttgatgg gcaacgaatt gtttgatgat
     4441 ataaaaactg atgcgtggga ggtgggctcg gctattgagt tatttcacaa ttttaccctg
     4501 atacatgatg atataatgga taaagctcct ttgagaaggg gaatggaaac aattcatgca
     4561 aaatatgggg agaatgtcgc cctgctggcc ggtgatgtga tgctggtgaa agcttaccag
     4621 caactggaaa aggttcaact ggagtttcag catcctatac ttcatttgtt taataaaaca
     4681 gctgctgaag tttgtgaagg ccagcaatgg gatatggatt ttgagaaaaa agaaacagta
     4741 aaactggatg attacctgcg tatgattgaa ctaaaaactt ctgtattatt agcagccagt
     4801 cttaagatgg gggccatatt aggtggggca ggagaaagaa atcaaaacct gctgtatgag
     4861 tttggaaaaa aactgggtat cgctttccag gtacaggatg attatctcga tgcttttggt
     4921 gaccctcaaa aatttggtaa acaggttgga ggtgatatac tagccaataa aaaaacattc
     4981 ttgctgatac atgcttttga gactgtaaat gatccgcaga aagatgaact aaatgaattg
     5041 ctcgcagcaa atgccccaga taaggtggaa aagatattaa agattttcag ggattgtaaa
     5101 gtggatgaat gggcactgga gttaaagaac aaatacctgg atgaagcatt ttcacatctc
     5161 gaagatattg ctgtagtttc aaaaagaaaa caaccgctga aggaattggg gcagttcctg
     5221 gtaaaaaggg aacattaagt ttgtttacaa taacctgtaa ctgctttcaa ttttttccta
     5281 ttttgaagca ttaaaaccaa ctgaagaatg ttaaatcaac ttttccgtaa gaaatctata
     5341 gccaaaattc tgcatgaagc agaggccggc ctgggcgacg ggcactctac aggtttaaag
     5401 aaagtgttag gggttagaga cctgactttc tttggagtag cggccataat aggcgcaggt
     5461 attttcagtg gtattggctc cgctgcttcc agcggtgggc cgggggtcgt gtttctctac
     5521 atttttacgg ccattgcctg cggctttgca gcactgtgtt atgctgagtt tgcgtccact
     5581 gttcccgttt ccggtagcgc ctacacttac tcttatgttg cttttggtga aattttcgca
     5641 tggatcattg ggtgggattt attaatggaa tatgccatag gtaatattgc tgttgccatt
     5701 tcctggagtg attattttac ccgcctgctt cactctgccg gaatgcatat tccggactgg
     5761 atgacgatgg attacatgac tgccaaagca ggtgcaacgg ctgaagcggt atctgcctga
     5821 acaaatgcac ctaatttatt tggcttaaag atagtaatgg atattcctgc attactgatt
     5881 gtggcactta taacttatat cgtttttgtt ggtattaagg agtcccgttc tgccagtaac
     5941 gttatggtta ttataaagct ggcagtaatc ttctttgtta ttgtacttgg ttctgtctat
     6001 gttaatcctg gtaactggtc accgtttact cccgaaggct ttggagggat aatgaaaggt
     6061 gtttctgctg tattctttgc ttacattggt tttgatgcaa tttctaccac ggcagaggag
     6121 tgtaagaacc cgcaaagaga ccttccaaag ggtatgatca attctttgat aatttgtaca
     6181 gtagtatatg ttttattggc tttggtgctt accggaatgg taaactataa gtcattaaat
     6241 gttggcgatc ccctggcaat ggtctttgat gcaaaaggtc tgaaatttat ttccggggtt
     6301 gttgctgtaa gtgccatcat cgctactgcc ggcgtattgt tagttttcca gctgggtcag
     6361 cccagaattt ggatgagtat gagccgtgat ggccttttgc ccaaaatttt ctcccgcatt
     6421 catcccaggc acggtactcc ttctttttct acgatcctca caggtatagt agtggcagtt
     6481 cctgccttat ttctgaacct tggagaagtg ctatcactga caagtatcgg tactttgttt
     6541 gcatttgtgc tggtatgcgg aggtgttctg gcgttagaac agcaaaagga caggcctgaa
     6601 agcaagttta aagtaccata tatcaattct cagtttatat accctgtact tttattggca
     6661 ggaataatcc tgatatgggt aaatgttccc agccatttta cagaggatat ctggacaaaa
     6721 gatacctggc caatggttgt tttttggctc attgcaatta ttatggccgt gatggccttt
     6781 gttaagcgat tttcgttgat cccgattttg ggtatgatga gttgttttta cctgatggcc
     6841 caggaaacac ataaggtctg gatgcggttc cttatctggc tggctatagg cttatgtgtt
     6901 tatttcctgt atagttacag aaatagcaag ctggcaaagg gaaaataatg tctcactatt
     6961 tcatttatag aatggcacag tgaagaagct gtgccatttg tatttttgca atatgaaata
     7021 ccagatcggc gatattgtgt tgattttaca ttccaatgag gaggcggaag tagtggacat
     7081 catcaatgat aaaatgctga tggtagatgt gaaaggggta tcttttccgg tgtataaaga
     7141 ccaggttgat tttccttatt tcaagcgatt tacagaaaag aaacctgtat ctgccaaaaa
     7201 ggaaaggaca tatatagatg atgtgcgaaa agaaaaaaga caggagcaaa acagggtaga
     7261 agatggagta tggctgacgt ttttaccggt aatggatatc gatgaattcg gagatgtgat
     7321 cgttgaggaa ctgaaacttc acctggttaa tcatacatca ttaccttata attttattta
     7381 taagctgcat ttctttggta agcccgattt tgagctaaaa aatacagtac aaccnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnat tgttaatacc tgataagaaa aaagcaggtc actttgaagc
     7561 atcagttaaa ttaaaaccaa agcaactctt tgccaaaatt gaagagttaa agcaaaagaa
     7621 ccttgctacc ttctcccagc ttttgtttga aaaatatccg gataaagctg tggaagatgt
     7681 tgtggagatg ggacgtatgg cggcaaaagg ctataaagtt tatgatgcct caaaggcccg
     7741 gcagcattta gaatctcccc gcagtgtaat tgatttgcat atagaaaaac ttaccgatga
     7801 ctggaagcat atgagtaatt atgaaatact cagcctgcaa ttgaaaacat ttgaaaaata
     7861 ttatcatctt tcagttatac atcatcaacc ttcactaata gtcatacatg gagtaggaga
     7921 aggggtgctt cgggatgaga ttcatgatat attaaggctt aaaaaagagg tcaaatcatt
     7981 tgtgaatcag tttcatccgg cttacggata cggagccact gaaatattct ttcaatactg
     8041 agttttgcag gctaatttta cccttgctac aagccaggct atcgcttctc cgattccagg
     8101 agaggaggaa agtccggaca gcacagagca atgcaccggt gaatagccgg gctccgggaa
     8161 accggtgaca gacagtgcca cagaaaataa cctcacccct caacccattt ctgcatctgg
     8221 agtgggttga ggggtgaaag ggtgaaaatg tgaggtaaga gctcacagct tttgatggca
     8281 acatcaattg cgggtaaacc ttgcatgctg aaatgccacg tataggatcg tttgagaact
     8341 gctcgttcaa gatccagggt aggcagattg atcctgcaaa gtaatttgcg ggacagataa
     8401 atgatagccc aagacagaat ccggcttaca ggcttgtagc ttttttattt aagttgatta
     8461 ccaataaacc cggtcatttc catcatccag gctattccta aatgaactat aagtcctccc
     8521 cagatagatc gggtattata tacaacgatt cccaatagca gcccgccgaa ataagaactg
     8581 atacattcgc ccaacggctt gccaaaatga atggtacaat aaaaacagtc cataggcagt
     8641 atggcatctt tacccaccca tttggcaaaa ccaagtacga ggaacccccg gaaaaaaaat
     8701 tcaatggtga agaaatcact tccataagat agttcgaata ataacttttg ccaccatatt
     8761 gcctctgagg aaacggaatt caattttgga tatactgaaa gaaaatcatt ttgtgttgcc
     8821 gccaaagtga ttaatggaaa catgattagt aacatcaaca aataaggttt ccatgccatg
     8881 tttccggttc ttaggccata aagtggctga tcttcgtgat acaatttcca tataaaataa
     8941 agtaagagaa aaataacaat cgcaagtgcc ggccaataac ctacctggtt ccaaaagtga
     9001 ttgtttgttt gattttctga aaatgatatt actaacgaga cgcttatttt ccacgcaaag
     9061 attaatggag taacgacaag taaaatgtag aagtttttgt tttttagatg gttctttttc
     9121 cttgcgaaac cataaaataa ataggggaca ataaatgctg cggcaaagac tacataccag
     9181 caaaatagtt taactacaaa taaatttttt tttctgatga atgtgtcaag tccaaaataa
     9241 taattacaaa agaccagaaa tgctgtaaac aaactgatca ataaaaggat agttctgtct
     9301 gtttccttgt aatagctctt aatatagtat atgatctcct tcatctcaaa cgaaaaaata
     9361 aaattttacg ccggtcttta aactttcttg ctccatcctt ttcaaaacat acgttaaaaa
     9421 tatttgaaac cccaaaagta tagccatgaa aactatcaca ctaaaccgga tgatgattgc
     9481 gatgttcgct gttttattat ccatctttgt tatctcctgt caaaaggaat ccagtctaac
     9541 cgaacctaca actttaactg aagaagaagc cgccacttat tcagaagaaa gtacccaggc
     9601 tgaagccagt tttgatgatg ctgaagatat tggttttaca gccgccgaag aagaaggtaa
     9661 tgcaggcggt tttggcctgg agggccgttc tgcatctgag cagggaagag ttttcctgcc
     9721 aaattttgct gaattgcgag gccgtattgg cgactgtgct acgattactg ttacaccaaa
     9781 cgacagcact tatcccaaaa cagtcaccat tgactttggc gacagttgcc gcggtcgtga
     9841 tggaaaagta agaagtggca agatcgtatt gcacttcaca gggcctatcc gtcgcccagg
     9901 ttctgtagta actatgacct ttgttaacta ttatgtaaac agggtacata ttgaaggaac
     9961 gaaaatattc aaaaacctga gtgaaccgcc aatgctgcat aaatggagtg tggaagtatt
    10021 ggatggtaag gtgactttcc ccaacggcag aggatacact tatgatggca ttaaaacagt
    10081 aaaacaaatt gccggtatgc ttaccaatgt tgtaagagat gatgtgtaca aggttaccgg
    10141 ccgttctaaa acagttttta gaaatgggac tacagtaaat atcaatactg aagatccgct
    10201 gatcaaaaag gtagcctgtc actggataag cgaaggcact ctgaagatta agatcaatag
    10261 cagaatttta ttccttgatt ttggtttccc caataacgga ggctgtgata ataaagccct
    10321 gttaacatgg aataatggaa ataaccagcg aatcattatc
//
LOCUS       DAORLS010000086        10355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3458_length_10355_cov_1.990971, whole genome shotgun sequence.
ACCESSION   DAORLS010000086 DAORLS010000000
VERSION     DAORLS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_10355_cov_1.990971"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK977_08180"
     CDS             complement(<1..394)
                     /locus_tag="PK977_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRF18130.1"
                     /translation="MSTTRRNFLRRMAIGTGAFAAGLPSFAKVAGATDEQMDRSTVLE
                     SNGQRFNMCGYAAPKIETVRIGIVGLGMRGSDAVERLSYIDGLEIVALCDKYPDRVAA
                     SQKILEKMGRPKAKEYSGEEGWKALCESN"
     gene            complement(498..2012)
                     /locus_tag="PK977_08185"
     CDS             complement(498..2012)
                     /locus_tag="PK977_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRF18131.1"
                     /translation="MLCHFIRVIAVSSILLLIVACSTTQKTTTTSTQKVTGSVMKEFR
                     AAWIATVANINWPSKPGLSTEEQKKEAIDLLDFLQKHHFNAAILQVRPQCDALYKSDL
                     EPWSYYLTGKQGQAPSPYYDPLEFWVEEAHNRGIELHVWLNPYRAHHKDGKEISDYSI
                     VKKRPELALYLKEGYWWLDPAQKGTQDHSTAVVMDLVDRYDIDGVHFDDYFYPYPSYN
                     LGADFPDSVGWNAYVQSGGKLSRGDWRREAVNVFIENLYKKIKVAKPHVKFGLSPFGI
                     WRPGYPESVSGFDQYEQLYADAKLWLNKGWIDYFTPQLYWPINRQNASFPVLLGWWES
                     ENTKKRHLWPGMSVGRDTSVKSVNETLNQIMITRGMVPESKGAVHWSISSVTKNASLA
                     KALLESTYRNDAIVPPSPWLDNKAPEAPKVTTAKNEKLTINWTHSDEKDVFRWVVYYK
                     YGNSWSHTILNRNDRSFSLSNSIEANKTTVPLKQIAVTAIDRTGNESSRTEITL"
     gene            complement(2073..>2765)
                     /gene="trmD"
                     /locus_tag="PK977_08190"
     CDS             complement(2073..>2765)
                     /gene="trmD"
                     /locus_tag="PK977_08190"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRF18132.1"
                     /translation="SYLCRMHIDILTVLPELVESPFQHSMMKRAQEKGLLTINVHQLR
                     KWAVNDYGQVDDYQYGGGAGMVMMCEPLAKAIDELSSERKYDEIIYMTPDGLTFNQKM
                     ANAFSLKENLLIICGHYKGIDERIRQHYITKEISIGDFVLSGGEIPAALLVDAIGRLI
                     PGVLNDETSALTDSFQDSLLAPPVYTRPVDFRGWKVPDILMSGNHKLIEEWRHEQALQ
                     RTKERRPDLLKD"
     assembly_gap    2766..2793
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2794..3158)
                     /locus_tag="PK977_08195"
     CDS             complement(<2794..3158)
                     /locus_tag="PK977_08195"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF18133.1"
                     /translation="MKAGYTYLFTALILVACSKNKESERDRVFPVITINTPVNGQSFT
                     AGQPISISGTITDNEYIAEVHIHVSNNNTGALLMDVHIYPGSNSTSFNESITASNGIN
                     YKIQVIAKDRTVNESRSVV"
     assembly_gap    3204..3214
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3409..>3601
                     /locus_tag="PK977_08200"
     CDS             3409..>3601
                     /locus_tag="PK977_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18134.1"
                     /translation="MKRAESIPVLIVSGLLLVYVLLVFRGKASGFAAFIFTFSPFLVI
                     WLVYSVIRHGEYKGRELESD"
     assembly_gap    3602..3618
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3653..4366)
                     /locus_tag="PK977_08205"
     CDS             complement(3653..4366)
                     /locus_tag="PK977_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF18135.1"
                     /translation="MLWPVLISGFTLGAAGSLHCVGMCGPLSLALPVSHLSKAQKFFT
                     LLLYQFGRVITYSLIGLVFGFAGRRIYISGYQQWFSIGLGIAVLILATLYFFRRFTVH
                     LGFLNRFYAAVQKMISRILKNAGSPFSFLLMGLANGLLPCGLVYVAVAATLSFTEVFH
                     SIGFMAMFGLGTLPAMMLVAYAGQKLKVETRELLRKAVPYFVVAMGLLLIMRGLNLGI
                     PFISPELPQSPGEAVVCHP"
     gene            complement(4366..4803)
                     /locus_tag="PK977_08210"
     CDS             complement(4366..4803)
                     /locus_tag="PK977_08210"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRF18136.1"
                     /translation="MNWGNKLLVTFIVFGLGMGYLVYRSMSTNFELVEKDYYKSELRY
                     QQVIDASNRTNELSTAVSLSQSENGITLQLPEEMKNKSITGDILFYCAYDKKKDKKFT
                     LRVNEDALQQFPLTDISSGNYTVKISWVHDGKTYYSEKSLTVL"
     gene            complement(4820..>6097)
                     /gene="ccoG"
                     /locus_tag="PK977_08215"
     CDS             complement(4820..>6097)
                     /gene="ccoG"
                     /locus_tag="PK977_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRF18137.1"
                     /translation="ALFFALPFIKLNGRPLFLFNIPDAKFIIFGKIFWPQDFFIFGLT
                     MVTFIIFIVLFTAAFGRLFCGWVCPQTIFMEMLFRKVEYLIEGDAAKQKILNKSSWTA
                     EKIRKKLTKHVAFFLLAFIIANFFLAYIIGIDELKKIISEPVSEHFVGFMAILVFSAV
                     FYGVYAFFREQVCTVVCPYGRLQGVLLDRNSMIVAYDYKRGEPRSKYKKGQTAELNTG
                     DCIDCFQCVKVCPTGIDIRNGTQMECVGCTACIDACDKMMDAVDRPRGLIRYASENGI
                     AEGKKLRYTGRMKFYTIILFVLTGLLSALLLSRKDIDGTIVRTRGQLYQERGTDSISN
                     LYNIKIINKTIKNIPVTLKLEGDFGKTGYIQLIGTENILLKEEDQATGSFFVVLPRNV
                     IDKRKLKLKIGLYEGDKKVTSISTNFMGPFSKL"
     assembly_gap    6098..6143
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6286..>6918)
                     /locus_tag="PK977_08220"
     CDS             complement(6286..>6918)
                     /locus_tag="PK977_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRF18138.1"
                     /translation="EEADLDLGHDYDGIRELDNRLPPWWLYGFYLTIIFAGIYLWRYH
                     VSHTAPSSKEEYERSVAKAEARIQEYMKKKGDAVDENTVKLLTSADDIAAGKAIFTDP
                     SKCVVCHGPEGGGNAIGPNLTDDYWIYGGNIKDLFKTIKYGAKNGMKSWKDDLSPKQM
                     AQVASYIKTLRGTKPANAREPQGELYKEEAAPSTPAADSTATKNNSVTMK"
     assembly_gap    6919..6928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6929..7474)
                     /locus_tag="PK977_08225"
     CDS             complement(<6929..7474)
                     /locus_tag="PK977_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18139.1"
                     /translation="MRKNLKYILGLFSGILLWAMQPVWAAGPPAPSPFSNPMIIMLVT
                     LMLLLLIVIGILANILIGAADVKLKRKKAMEKGQSAAALLVIFMLLSSTGVFAQGTTP
                     ETTATAAKTIAGMSASTFYIMTGVLFIELMIIIGLLINIKFLLKTEKEKFVTETATPE
                     QIAEIKRNKLSWWDRFNKLKPV"
     gene            complement(7505..7666)
                     /locus_tag="PK977_08230"
     CDS             complement(7505..7666)
                     /locus_tag="PK977_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HRF18140.1"
                     /translation="MKFINYLEKVSGVEIMGLVSLLIFVVFFSVMLTMVFRTKKKELQ
                     EISRIPLDN"
     gene            complement(7678..>8035)
                     /locus_tag="PK977_08235"
     CDS             complement(7678..>8035)
                     /locus_tag="PK977_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HRF18141.1"
                     /translation="DKNKRPDSWHYTHMYDPRLTSPGSLMPRYIWLLDNKIDTASTPA
                     KIRAMKTLGVPYPDGYDKIANRDLMHQADSIASNLKKDKIETASNVEVIALIAYLQRL
                     GRDIQGEAKKPIAENK"
     assembly_gap    8036..8057
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8058..9827)
                     /gene="ccoN"
                     /locus_tag="PK977_08240"
     CDS             complement(<8058..9827)
                     /gene="ccoN"
                     /locus_tag="PK977_08240"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRF18142.1"
                     /translation="MQVEKFYYDNKIVKAFGLATILFGIVGMLAGLWVAIQIYYPESS
                     LNLAATTFGRLRPLHTNAIIFAFVGNASFAGIYYSLQRLCKARMFSDKLSWIHFWGWQ
                     LIIAAAAITLLMGKTTGKEYAELEWPIDIAITLVWVIFGINMFGTIIKRRERHLYVAI
                     WFYIATWVTVAMLHIVNSIEIPVSALKSYSWYAGVQDALVQWWYGHNAVAFFLTTPVL
                     GLMYYFLPKAANRPVYSYRLSIIHFWALIFIYIWAGPHHLLYTALPDWAQSLGVVFSV
                     MLIAPSWGGMLNGLFTLRGAWDKVREDPVLKFMVVAITCYGMSTFEGPMLSLKNVNAI
                     SHFSDWTIAHVHIGGLGWNGFLAFAILYYMIPKMWGTNLYSKKLATNHFWLGTIGIIL
                     YAVPLYWAGFEQSLMWKQFTPEGQIKYDFLQTVTNIKPMYIARSVGGSLYLIGALMMV
                     YNIYKTIVSGKFIANEAAEAAPLVKEAHPKKEYWHRVIERKPVTMLVLSLILVAIGGL
                     VQMIPTFLVKSNVPTIASVKPYTPLELQGRDIYVREGCYTCHSQMIRPFRDEVARYGE
                     YSKAGEFVYDHPFQWGSKRTGPDL"
     gene            complement(9847..10017)
                     /gene="ccoS"
                     /locus_tag="PK977_08245"
     CDS             complement(9847..10017)
                     /gene="ccoS"
                     /locus_tag="PK977_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRF18143.1"
                     /translation="MSVILILLIASISVAGLFLAAFLWGVRSGQFEDDYSPASRILFD
                     DKPSSKTKTQSQ"
     gene            complement(10172..>10355)
                     /locus_tag="PK977_08250"
     CDS             complement(10172..>10355)
                     /locus_tag="PK977_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18144.1"
                     /translation="KNIVIASFVLSIVYNIIGLSFAVQGTLSPLIAAILMPASSLSIL
                     LLTFGSSSWLARQMKL"
BASE COUNT         2864 a         2214 c         2046 g         3097 t
ORIGIN      
        1 tattactttc acagagtgct ttccagcctt cttcaccgct atattctttt gccttaggcc
       61 ttcccatttt ctccaatatt ttttgagatg ctgctacccg gtctggatat ttatcacaaa
      121 gggctactat ctctaatcca tctatatagc ttaatctttc caccgcatct gaaccccgca
      181 ttcctaatcc aactatacca atgcggacag tttcaatttt tggtgcggcg taaccacaca
      241 tattaaagcg ctgaccatta ctttcaagta ctgtggagcg gtccatttgc tcatcagtag
      301 cgccggctac tttggcaaaa gatgggaggc cggcagcgaa agcgccggta ccaatagcca
      361 tacggcggag gaagtttctg cgggtagtac tcatacggga agcatttgtg taaacgcaag
      421 ttcttgaatt aatcagaata aaagcggcga tcaaaaaaat gaaacaatcg tttgctgagt
      481 actgttgttt gtatttttta aagggttatt tctgttctgc tgctttcatt tcctgtacgg
      541 tcaatagcag tgacagctat ttgctttaat ggaactgttg ttttattcgc ttcaatactg
      601 ttgcttaaac taaatgacct gtcattcctg ttaagaatgg tatggctcca gctgtttcca
      661 tatttgtaat aaaccaccca tctgaaaaca tctttttcat cggaatgggt ccagttgatt
      721 gttaattttt cattttttgc agttgttacc ttaggagctt ccggggcttt attatccagc
      781 caggggcttg gaggaacgat tgcatcattt ctgtatgtgc tttcaagtaa tgctttggca
      841 agacttgcgt tttttgttac agatgatatg ctccagtgta cagccccttt gctttcagga
      901 accatacctc tggttatcat aatctggttc agtgtttcat ttactgattt tacacttgta
      961 tctctcccta cactcattcc cggccataaa tgtctttttt tcgtgttttc agattcccac
     1021 caccctaata gtacagggaa acttgcattc tgcctgttga taggccagta taactgggga
     1081 gtgaaataat caatccatcc tttgttcagc caaagctttg catcagcata taattgctcg
     1141 tactggtcaa acccgctgac tgattcaggg taacccggac gccagatccc aaaaggactc
     1201 aaaccaaatt ttacatgtgg ctttgccact tttatttttt tatacaaatt ttcaataaat
     1261 acattcaccg cttcacgacg ccagtcgcct cttgatagct tacctccact ttggacataa
     1321 gcattccagc ctactgaatc ggggaaatca gcccccaggt tgtaagaagg atatgggtag
     1381 aagtaatcat caaaatgaac accatcaata tcatatctgt caacaaggtc catgacaact
     1441 gcagtggagt ggtcttgtgt gcctttttgt gccggatcga gccaccagta gccttctttt
     1501 aaatacaggg ccagttccgg tcgttttttc acaatggaat agtcgcttat ttcttttcca
     1561 tccttatgat gtgccctgta gggattcagc caaacatgca gttcgattcc acggttgtgc
     1621 gcctcctcaa cccaaaactc aagtggatca taatagggcg atggagcctg tccttgtttg
     1681 cctgtcaggt aataggacca tggttcaaga tcgcttttgt atagggcatc gcattgaggt
     1741 cttacctgca ggatggcagc attgaaatga tgtttttgta aaaaatcaag taaatctatt
     1801 gcttcttttt tttgttcttc cgtactcaaa cccggcttgc tgggccagtt gatattggct
     1861 acagtggcaa tccatgctgc cctgaactcc ttcattacac tgccggtaac tttttgtgtt
     1921 gaggttgtag ttgttttctg tgttgtgctg caggcaacta tcagtaacaa tattgaactg
     1981 acagcaataa cccgaatgaa atgacataac attttgctta ctttaagatg atgttattga
     2041 aggttcttta aaagtatggt aaaatggcag gattaatcct taagaaggtc gggtcttctt
     2101 tcttttgttc tttgcaaagc ctgctcatgc cgccactctt caatcagttt atgatttccg
     2161 ctcattaaga tatcgggaac tttccatcca cgaaaatcca caggacgggt gtatacgggg
     2221 ggtgccaata agctatcctg gaatgaatct gtaagggcag aggtttcatc attcaataca
     2281 cccggtataa gtcttccaat tgcatccacc agcaaagcag caggtatttc gccgccgctt
     2341 agtacaaaat ctccaataga gatttctttg gttatataat gctgacggat acgttcatca
     2401 attcctttat aatgaccgca aatgatcagc aggttttctt tcagggagaa agcattggcc
     2461 attttctgat tgaatgttaa tccatcggga gtcatataaa tgatctcgtc gtattttctt
     2521 tctgaggata attcatcaat agcttttgcc aggggttcac acatcattac catacctgcg
     2581 ccaccgccat attggtaatc atccacctgt ccgtaatcat tcacggccca tttgcgtagt
     2641 tgatgaacgt taatagtaag cagtcccttt tcctgggccc ttttcatcat gctgtgctga
     2701 aaaggacttt ccaccagctc aggaagtaca gtcagaatat cgatgtgcat gcggcaaaga
     2761 taggannnnn nnnnnnnnnn nnnnnnnnnn nnntctacaa cggatcggct ttcattcacc
     2821 gtcctgtctt tggctatcac ctgtatttta tagttgatgc cgtttgaagc agtaattgac
     2881 tcattaaaac tagttgagtt cgacccggga tagatatgta catccatcaa taatgccccc
     2941 gtattgttat tgctgacatg tatatgtact tcggcaatat attcattgtc agtaatggtg
     3001 ccgctgatag atataggctg gccagccgta aatgattgcc cgtttacagg agtattgatg
     3061 gtgataaccg gaaagactct gtctctttca ctttctttgt tcttagaaca agcaactaaa
     3121 attaacgctg taaataaata ggtatagccg gccttcattc ttcaaagtta tactaaaaga
     3181 aacccccgga gagccggggg tcgnnnnnnn nnnnccaaca ataactcatt ttcatgagtt
     3241 acataagtgt atgtaatact ttcacctgga aacaaagcta atatgaggta tctcgtaaaa
     3301 aactgattat cgtcaattaa agtattgatt gcggtcaggt tataaataca agaagaatta
     3361 ctaatgtgat tgttccgaac tttaattctg caaagatgtt gatgctatat gaaaagagct
     3421 gaatcaatac ctgttctgat cgttagtggt ttacttcttg tctatgttct gctggttttt
     3481 cggggtaagg cctctggttt tgcagctttt atttttacat tctcaccatt tttagtgatt
     3541 tggctggtat attctgtaat ccgacatggt gaatacaaag gcagggagct agaatctgat
     3601 gnnnnnnnnn nnnnnnnntg atgaggagtt tggctatacg gataaaccgt gatcagggat
     3661 ggcaaacaac cgcttctccc ggtgattgtg gtaattcagg gcttataaat gggattccga
     3721 ggttcagtcc ccgcattata agtagcagtc ccatagccac tacaaaatac ggcacagctt
     3781 ttctcaatag ctctctcgtt tccaccttca gtttttgacc ggcataggcc actaacatca
     3841 tggcaggcag agtacccagg ccaaacattg ccataaaacc aatgctgtga aaaacttctg
     3901 tgaaagacag ggtagccgct acagcaacat atacaaggcc gcagggtaat aatccattgg
     3961 ccagccccat taaaagaaaa ctaaaagggc tgccggcatt tttcaatatt ctgctgatca
     4021 ttttttgtac agcagcataa aacctgttca ggaaaccaag atgaacagtg aaccggcgaa
     4081 aaaagtataa agttgccaat atcagaactg caattcccag cccaatagaa aaccattgtt
     4141 gatacccgga tatataaatt cttctgccgg caaagccgaa tacaagcccg atcagggaat
     4201 aagtaataac tctgccaaac tggtagagta gtaaggtgaa aaatttttgt gcttttgaaa
     4261 gatgactcac aggtaatgcc aggctaagcg ggccgcacat acctacacaa tgcaggctgc
     4321 ctgctgcccc taatgtgaat cctgatatta ataccggcca tagcattata aaacggtcag
     4381 gcttttttct gaatagtatg ttttaccatc atgtacccaa ctgatcttta ctgtatagtt
     4441 gcctgaagaa atgtcagtta atgggaattg ctgtaacgca tcctcattaa cccgcaaggt
     4501 gaatttttta tcttttttct tgtcataagc gcaatagaat aagatatcac cggtgatact
     4561 cttgttcttc atttcttcag gaagttgtag tgtaatgccg ttttctgatt gcgacaagga
     4621 tacggctgtg ctgagttcgt tggttcggtt agaggcatca atcacctgct ggtagcgtaa
     4681 ttcgctcttg tagtaatctt tttcaaccag ttcaaaattc gtactcatag agcgatacac
     4741 caggtagccc ataccaagtc cgaaaacgat aaatgttacc agtaatttat ttccccagtt
     4801 catattgtat tgttggtttt tatagtttgc tgaaaggtcc catgaagttt gtgctgatag
     4861 aagttacctt cttatcacct tcatataagc ctattttcag ttttagtttc cttttgtcaa
     4921 taacatttct cggtagaaca acaaaaaatg aacccgttgc ctggtcttct tccttcagca
     4981 gtatattctc tgtaccgatc aattgtatat acccggtctt gccgaaatca ccctccagtt
     5041 ttaatgtaac gggaatattt ttaattgtct tgttgatgat tttaatgttg tacaggttag
     5101 aaatactgtc ggtacctctt tcctggtaaa gctggcctct tgtgcgaacg atagtgccat
     5161 ctatatcttt acggcttaac aacagcgcag aaagtaatcc agtaagcaca aacagaataa
     5221 tcgtatagaa tttcattctg ccagtatacc ggagtttctt tccttcggcg ataccatttt
     5281 cagacgcata gcgtatcagg cctcttggcc tgtctactgc atccatcatt ttgtcacagg
     5341 catcaataca ggcagtacag cctacgcatt ccatttgtgt gccattacgg atatcaatac
     5401 ccgtaggaca aacttttaca cattgaaaac agtcgatgca atctccggta ttcagttccg
     5461 cagtttgtcc ctttttatat ttactcctgg gttcgccccg cttataatcg tatgcaacaa
     5521 tcattgaatt cctgtcaaga agtacaccct gtaatcgtcc gtagggacaa acaacggtac
     5581 aaacctgttc cctgaaaaag gcataaacac cataaaaaac agcagaaaaa accagtattg
     5641 ccatgaagcc aacaaaatgt tcgcttacag gttcacttat gatctttttt aattcatcta
     5701 tgccaataat ataggcaagg aagaaattgg caatgatgaa tgccagcagg aaaaaggcaa
     5761 catgttttgt tagctttttc cggatctttt cagctgtcca tgatgatttg ttgaggattt
     5821 tttgtttggc agcatcacct tcaatgagat attccacttt tcggaatagc atttccataa
     5881 aaatggtttg cgggcagacc cacccgcaaa ataaccttcc aaaagctgct gtaaaaagta
     5941 caatgaaaat gatgaaagtt accatcgtca gcccaaatat gaaaaaatcc tggggccaga
     6001 aaattttccc aaagatgata aacttggcat cggggatatt gaacagaaaa agcggtcgtc
     6061 cgttcaactt aataaacgga agtgcaaaga acaaagcnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnntagaatt ttcctttagg cttttgggca aagacccatt
     6181 tcctttttcc tttctcatca accgttgcta ttctgtccct gaaggactcg ctggatgtat
     6241 tctcagtatc taaattattc tttgcctgct tttccatttt tcctcttatt tcatcgtcac
     6301 tgaattgttc ttagtggctg tagagtcagc agcaggcgtt gacggtgctg cttcttcttt
     6361 gtataattcg ccttgtggtt cccttgcatt ggcaggtttt gtacctctca gcgtcttaat
     6421 atagctggct acctgtgcca tttgttttgg agacagatca tctttccagc ttttcatacc
     6481 attctttgct ccatacttaa tcgttttaaa cagatccttg atattgccac catatatcca
     6541 gtaatcgtcg gtaagattag ggccaattgc attgccacca ccttcagggc catggcaaac
     6601 aacacattta gacggatcag taaaaattgc ttttcctgca gcgatgtcat cagcactggt
     6661 taataattta actgtattct catcaacggc atcacctttt ttcttcatat actcctgtat
     6721 ccgggcttca gctttggcca cagatctttc gtattcttct ttactggaag gagcagtgtg
     6781 agagacatga tagcgccaca ggtagatgcc tgcaaaaatg atggttaaat aaaaaccgta
     6841 caaccaccag ggtggaagac ggttgtctag ctcacggata ccatcataat catgtccgag
     6901 gtccaggtcg gcttcttcnn nnnnnnnnaa cgggtttcaa tttattgaac ctgtcccacc
     6961 agctaagttt atttcttttg atttcagcga tctgttcagg agttgcagtc tcggtaacaa
     7021 atttctcttt ctctgtttta agaagaaatt taatatttat taataacccg attatgatca
     7081 tcaattcaat aaaaagtaca ccggtcatga tgtaaaatgt acttgctgac atgcctgcta
     7141 ttgtcttggc agcagtagca gtggtttctg gagttgtacc ctgtgcaaaa acgcctgtac
     7201 tgctgagtag cataaaaatc accagcaaag cagcagctga ctggcctttt tccatggctt
     7261 tcttcctttt tagttttaca tcagccgcac cgatcaggat gttagccagt ataccaatga
     7321 caattagtaa aagcaacatc aatgttacga gcatgatgat catgggatta ctaaaaggag
     7381 atggtgctgg aggccctgca gcccaaacgg gttgcatagc ccagagcaat atccctgaga
     7441 aaagaccgag tatgtatttt aaattctttc gcataattat aagcttattg gttagtttaa
     7501 ttgcttagtt gtctaatgga atacggctta tttcctgtaa ctccttcttt ttggttctga
     7561 ataccatcgt cagcattacc gaaaagaata cgacaaatat taataaggac acaagcccca
     7621 ttatttccac accgcttact ttttctaaat aattaatgaa tttcatgatt cagtttttta
     7681 tttgttttca gcaatgggtt ttttagcttc tccttgtatg tcccttccta aacgctgcag
     7741 gtaagcaata agagctatca cttcaacatt ggaagctgtt tcgatcttat cttttttcag
     7801 gttggaagcg atagaatctg cctggtgcat caggtcgcgg ttggcaattt tgtcatagcc
     7861 atccgggtaa ggcacaccga gtgttttcat tgctctgatc ttggccggtg tagaagctgt
     7921 atcaattttg ttatccagga gccagatata tcttggcatt aaggagccgg gtgatgtaag
     7981 tcttggatca tacatgtgtg tgtaatgcca ggagtcaggc ctcttgtttt tatcgnnnnn
     8041 nnnnnnnnnn nnnnnnncaa atccggaccg gtacgcttac ttccccactg gaacgggtga
     8101 tcataaacaa actcaccggc cttggaatac tcaccgtatc tcgccacttc atcacggaac
     8161 ggacgaatca tttgagagtg gcaggtgtaa caaccctctc tcacataaat atcacgacct
     8221 tgcagctcaa gtggtgtgta tggttttaca ctggctatgg tcggcacatt tgattttacc
     8281 aggaatgtcg ggatcatttg taccaggccg cctattgcca caaggatcag gctcaatacc
     8341 agcatggtaa caggttttct ttcaattacc ctgtgccagt attctttctt aggatgagct
     8401 tcttttacca atggtgctgc ttctgcggct tcattggcaa taaacttacc gctgacgata
     8461 gttttataaa tattataaac catcattaat gctccgatca ggtataatga accacccaca
     8521 ctacgggcaa tatacatggg cttgatattg gttaccgttt gcaggaagtc atacttgatc
     8581 tggccttccg gggtaaattg tttccacata aggctctgtt caaaaccagc ccagtacaag
     8641 ggaacagcgt ataaaatgat accgatagta cccagccaga agtgattagt ggctaatttc
     8701 tttgaataaa gattggtgcc ccacattttc ggaatcatgt aataaagaat agcaaaagca
     8761 agaaatccgt tccagcctaa tcctccaatg tgtacgtggg ctatggtcca gtcgctaaag
     8821 tgagaaatgg catttacatt tttcaaactc agcatcgggc cttcaaaagt gctcatacca
     8881 taacaggtaa tcgctaccac cataaatttt aaaacaggat cttctcttac cttatcccag
     8941 gcgccacgga gagtgaacaa gccattcagc ataccgcccc agctcggtgc aatcagcatt
     9001 acggagaata ccacacccag tgattgtgcc caatcaggca aagctgtata taaaaggtgg
     9061 tgagggccgg cccagatata aataaatatc agtgcccaga agtgaatgat agaaagtctg
     9121 taagagtata ccggcctgtt ggctgctttg ggaagaaagt agtacatcaa acctaaaacc
     9181 ggggtagtaa gaaagaaggc cacagcatta tgtccgtacc accattgtac caacgcatct
     9241 tgcacaccgg cataccagct gtaacttttc aatgctgaaa caggaatctc aatagagtta
     9301 acaatatgca gcatggctac agtaacccag gtagcaatgt aaaaccagat agctacatac
     9361 aggtggcgtt cccttctttt aataatggtg ccaaacatat tgataccgaa gattacccat
     9421 acaagggtaa tagcgatatc aataggccat tccagttcgg catattcctt accggttgtt
     9481 ttacccatca gtaatgtaat ggcagcagca gcaataataa gctgccagcc ccagaaatga
     9541 atccagctca gcttgtcact gaacattctg gctttacaaa gacgttgtaa ggaataataa
     9601 atgccggcaa acgatgcatt acctacaaag gcaaaaatga tggcattagt atgcaacgga
     9661 cggagacgtc caaatgtagt agcggcaaga ttgaggctgc tctccggata gtagatctgg
     9721 atagcaaccc ataagccggc cagcatacct acaataccga ataagatcgt tgccaaacca
     9781 aaggctttaa caatcttatt gtcataataa aatttttcta cttgcataag ctggattgtt
     9841 tttaatttat tgtgattgcg ttttggtttt actggaaggt ttatcatcaa acaggatacg
     9901 ggaagcaggc gaataatcat cttcaaactg accactgcgt actccccaca ggaaggcagc
     9961 aaggaaaagg ccggcaacag aaatgctggc gataagaagt attaagatta cactcatgtc
    10021 ctgttttttg gacccgatcc ttcgactact gctcaagact atcgggattt gtaaaagtat
    10081 tttgagggta tagggtgtcg ggtaataatt atcactcagg gaactgattt ttatcatttc
    10141 cctgttcaaa tagcctgtaa tacagtgatt tctaaagttt catctgcctt gctaaccaac
    10201 tgctgctacc aaaagtcagc aacaaaatgc ttaatgaact tgccggcatc agtatggcag
    10261 ctatcaaggg cgacaaagtg ccctgtacag caaatgataa accgataatg ttgtaaacaa
    10321 tagaaagaac aaaactggct atcacgatat tctta
//
LOCUS       DAORLS010000087        10342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3464_length_10342_cov_1.828327, whole genome shotgun sequence.
ACCESSION   DAORLS010000087 DAORLS010000000
VERSION     DAORLS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_10342_cov_1.828327"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK977_08255"
     CDS             <1..527
                     /locus_tag="PK977_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18145.1"
                     /translation="NYKVTYYNLCGDGPASNIVSFAVNSVPQTINFPSIPNKTYGDSA
                     FAIIATASSGLPVSLALISGPGSLSGNTYTITTTGTATIRAIQPGDDVYDTAAIVLQQ
                     IIINKAAQNINFPIIPDQDINSTPFTLTATASSGLPVSYSLISGPGVLSGNQLTYTGV
                     GTISITALQPGDTN"
     gene            571..5421
                     /locus_tag="PK977_08260"
     CDS             571..5421
                     /locus_tag="PK977_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRF18146.1"
                     /translation="MINGPQYVCPGQTATYRINKVTGLTYSWRLSDGTTYPSTADSVN
                     ITWGSSGVKTLIVSASGPCGAASANDSLVVNIITAQTPGAVSNMLPADGATEQILPLN
                     LSWQPGSNALSYDLFVWDSSATEPSTPYAANLTNVSYIIPQGALPYNKAYKWKVVSKN
                     ACLETSGPVQYFRLKKLPDLLVSNVQAPTTAFSGQTITINWRVANAGPGNTATNQGWT
                     DAVFLSFDSLPNFSISPNTSPAAWNQLELPIRPLLIGTRNNISALDSGQHYDNNINFT
                     LPINYNQPLYVYVITNYPANTSLQQMTVINDTARAPQAIAVSLSPTADLRVDTVFTPS
                     SVFSGSTIDITYKVKNYGVMTPAGSVWTDKLYISQSPIFNINNATQLKLPRINGTYYP
                     NAENANITNNTQLLADSSYTRTVQAVIPNFISGQWFIYVHTNATTSLYEGALANNNIN
                     NKLLHVFLTPTPLLTINSLSVPLTVASTTQPIGINWNIFNTGFNDNLEKNKGHYYRHT
                     GFCQSNLLQLTDSAGFGSSYWVDRVYLSTDSTGINGNAILIGEKPQGVINSGIHADNA
                     PSYKCVHPGTNPASENINTFNVIRPGSNHPGEFNFTIPADLLPGNYYVYVRANATQTV
                     YEYPGTPQIRRSALPINIRRPDLAVSATVPASASAGQPVTITYSILNNGPGTVFNQLR
                     NDKIYFSTSPVFDGAAQLINTQSFTESIPAGTPVSHTFSYTIPNGVSGNRYFYVHTNF
                     DSTFRETNYSNNISSAAPTSVINPTPADLVVTSVPLADSLFTIYNSKIKYTVTNNGPG
                     STYGTWTDSIFVSCNPVYSSANSYFVAKRNHSGTLNTGTSYTDSVNLNMQFSWTYNSC
                     FPEIAATTAYFFVKTNASGDVYEGANSNNNVNGSGARILVNPLVDHIVTSISGSDTAT
                     AARPYSINWTVKNIGRNPGSVYYQLWNDGVYFSPDSLENGNEILASQFLMTTRLESNQ
                     SYSETRPAIVPNIPTGDYYVFLKTNVTNYIPGEKQLNNNSNFIRNITGAARKIHVIQP
                     LLPDFVDTIFSAPATAAAGQPATVIHKITNSGAGPVYPNNWSTKLWLSTDFTPGNSGD
                     IELFSRNMNAALQPGQSLNDTITFTLNLNTVPGNYILIAHTDANSSVFESNDTNNLAF
                     SYVTVFRTQPVDLVVENIMKPDTVLLGYSIDTAKWVIANLSANTATGVSSDGIYLSSN
                     TVLDSTAVLVGIKNKNINMPPLDRDTISMQPLVTSLVEGQYKVLVKTDLLNNINETNK
                     NNNTGISAGSIYVKAKELKLDIPELNTLHTLTRYYKLVIPDSLNGSTILVSLNTSDSL
                     SMRNEMYIGLGYVPSSARFDYKFNTPNYGNQQIVMTSVVDSVYYIAVRCVSSNPLIQN
                     ITLTAVRLPFTILNVQSSTGGNIGNVTVKLSGSLFTNNMLARLSRPGTTIYSSAVYFI
                     NSTTVFATFNLQGRPVGLYDVSLHKQDSSVAVLAGSFTIATGNNGGILTGGGNNTGQT
                     GSGNQPGCDPGTPGGLNSLLVTEVVVPEKVFVGWPFVIQINYANPANFDIPAQTRTLY
                     NDKNVKMAMTQAALANGSNSLHLELTEPGGPPGIIRAGASGTITVYGKTPPDTPGHTL
                     INFTLR"
     assembly_gap    5453..5514
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5564..>8096
                     /locus_tag="PK977_08265"
     CDS             5564..>8096
                     /locus_tag="PK977_08265"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HRF18147.1"
                     /translation="MGLEVNTLTGNLYFTRNDLYIPARGFDLNITFHYNSFNYDKNDG
                     YGKGWSFNYNIKYKNDTLGSKTIIWGDGREDNYLPEGAGFKAPRGFFSTLSEYQPGKY
                     RITETDGTKYFFDNNIHKRITRMEELNGNFINFNYSDTLLTALTNGAGQTISLAYTNG
                     RLTSITDAMTVPTRVISYIYDGAGNLVEVKDPLNNATKYAYLVNGPMKAVTDKNNNKV
                     DIIYFNNFSISELIGCNKRLSLSYDSATHTTMVTDYLETGSNQVTKYEFKKQEDKKWL
                     SGMSGNCCGFNMKFEFDAAGNKIKETDANGNVTTFTYDNRGNMLTMTDALNNTIKYTY
                     TTDFNRVASFTDEKGYTTTMTYDVKGNLTQLTEPGNHVYTASYAANGDIQSSTDPKGN
                     SFSYNYDTYGNPVNVTGPNGYSAQLVFDARGNLLSVTDARGNTSNLEYDILGRLKKIT
                     DPINNNIQLNYDAEGNPVSLVNENNETSLSAFDASNRLVEFTDPTGGKTTLTYDAMDN
                     IKSIRNTLGNALSFEHDSRNRLKKITDPEGNVLSSDYDANGNLTSVNMPNGQVFSYSY
                     DALNRIIAANDINGQVISLTYDKNSNVTSFTNGSGATYSAEYDSLNRVKKITDPLSNT
                     YTLLYDKNSNIVAVTDRNGFTSTYTYDSLDRVKTTTDNNGNTITVNYDFQGNITALTD
                     QNNNTTSYTYDNLNRLKRTTYPDGKYTENSYDNKSNIIAKRITDGTTITFTYDTLGRV
                     ISKTLPDGHVYTYTYDALGRLLTATNNAGTVTLTYDALNRLTSENFGNRTVNYTYNIA
                     GRTQSTIYPDSTVITKSYDTRNRLISIIKNNSVLVSYQYNNANQLI"
     assembly_gap    8097..8237
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8238..>9319
                     /locus_tag="PK977_08270"
     CDS             <8238..>9319
                     /locus_tag="PK977_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF18148.1"
                     /translation="KTSINRLNDPARSEQFSYDNGRRLTNYKRGAIGGVPVVENTYNY
                     DALGNRTSASLNGTNTTYTSNNLNQLTSSNNGTQNINFTYDNNGNLTYDGQFYKFYDP
                     EKKLLKDSASPSQVYVYHYDALNRRFRKNLNGTNRYYTFSGITPIEERDGTGNTILNK
                     TIYAGWLSPVVNEKNNNQFFYHQNELNSVEAITNQQGRLIEKYNYDAYGKMSIYDSLN
                     NALPGSLTGNRFGFTGQEYDSTTGKYKFFFREYNPETGLFSQRDLIGYADGMGMYQYV
                     HNNPANGVDVLGLEDCPPDKAKNTQVTLRQSVEELETLGSNYTTVLSLINQFRIEFNI
                     AKNSKLDVLIDGLIDAGDFVKANELA"
     assembly_gap    9320..9345
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9346..>10342
                     /locus_tag="PK977_08275"
     CDS             <9346..>10342
                     /locus_tag="PK977_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18149.1"
                     /translation="ANELKGLKGLSSGLGKLSFGLTGIDALFKGNTLGNVLNDPNSGT
                     TEIAKASGDLAQSLLSFSGIGSVYGLFDFAQERLITGKSMNALSAEYGENLGDNSVDQ
                     TMDEEEQEFHRKLGPEHFKKWLKFRNQTNMREARKLRNINCPQNSSGGTQKPASIIAG
                     GISDIAEAISSWDPNEIIGPDGVPDKSWVSINDRLPYTVTFENDKSASAPAKYVKVVV
                     PVHEKMDAASFQLGSFGFNSLTFTVPPAAASYYQRLDCRDSLGLYVDVIAGYDVVNNH
                     AFWEFQSIDPVTFLPPADPLKGFLLLQDSSNLTYGHGFVNFSIKPVSTAQTLDSIL"
BASE COUNT         3241 a         2179 c         2014 g         2679 t
ORIGIN      
        1 gaaattataa ggttacttat tataatcttt gtggtgatgg tcctgcatcc aatatcgttt
       61 catttgcagt aaactctgtg ccgcaaacca tcaatttccc gtctataccc aataaaacgt
      121 atggcgactc tgcttttgcc attattgcaa cagccagttc cggacttcct gtatcattgg
      181 cgctgatcag cggaccggga tctttatccg gcaacactta tacaataaca acaacaggca
      241 cagccaccat tcgggctata caaccgggcg atgatgtata tgatacagcc gcaattgtat
      301 tacagcaaat aattattaac aaagctgctc aaaacatcaa ttttcctata atacctgacc
      361 aggatattaa ctccacacct tttaccttaa cggctacagc aagttctggc ttgccggtta
      421 gttattcgtt gatctcaggt ccgggagtat tatcaggaaa ccagcttacc tatacaggag
      481 tcggcacaat cagtattacg gcgcttcaac cgggtgatac aaactaattc agtaaacaga
      541 acattctgtg ttagggtagc tgaactcaat atgattaacg ggccacaata cgtttgccct
      601 ggtcagaccg ctacttatcg aattaataaa gtgaccgggc ttacctatag ctggcgtctt
      661 tcggatggca ccacttaccc atctactgct gattcagtga atataacctg gggatcttca
      721 ggcgttaaaa cactgattgt ttcagcatcc ggtccatgtg gtgcagcatc agccaacgat
      781 tcactggttg taaatattat tactgcacaa acacccggtg cggtttctaa tatgcttccg
      841 gctgatggag caacagaaca aatattgccg ctgaaccttt catggcagcc gggcagcaat
      901 gcgcttagtt atgacttgtt tgtgtgggat tcatcagcta cagagccatc aacaccttat
      961 gcagccaatc ttaccaatgt atcttatata attccccagg gagcattgcc ctataataaa
     1021 gcctacaaat ggaaggttgt atcaaaaaat gcttgtcttg aaacgagtgg cccagtccaa
     1081 tacttcaggc tgaaaaaatt gccagatctg ctggtaagca atgtacaggc gccaacaact
     1141 gccttttcag gacaaaccat aactatcaac tggagagtag ctaatgcagg accagggaat
     1201 acagccacta accagggttg gactgatgct gtttttcttt catttgacag cctgcccaac
     1261 tttagtattt cgccaaacac atcaccagcc gcatggaacc aactggagct tccaataagg
     1321 cctttgctta ttggtacccg aaataatata tcagcgctgg atagtggcca gcattatgat
     1381 aacaatatca atttcacatt gccgataaat tataaccagc cgctttacgt ttatgtgata
     1441 acaaattatc ccgcaaacac cagcctgcaa caaatgactg tgataaatga cactgcaaga
     1501 gcaccgcagg caatcgcagt atcactttca cctacggcgg atctgagagt tgacaccgta
     1561 tttactccat cttccgtatt ctcaggcagc actattgata ttacttataa agtaaagaac
     1621 tatggagtga tgactcctgc cggcagtgtg tggactgata aactttatat ctcacaaagc
     1681 cccattttca acatcaataa tgccactcag ttgaaactgc caaggattaa tggaacttat
     1741 tatcccaatg ctgaaaatgc gaacattaca aataacacac aattgctggc tgacagttca
     1801 tataccagga cagtacaggc agtgattccc aattttattt cgggtcagtg gtttatctat
     1861 gtccatacga atgctactac aagtttatat gaaggtgccc tggccaataa taatatcaat
     1921 aataaattgt tgcatgtgtt tctgactcct acacccctgc ttactattaa tagcctttct
     1981 gttcctttga ccgttgcaag cactactcag ccaattggaa taaactggaa catatttaac
     2041 actggtttta atgacaacct tgagaaaaat aagggtcatt actacaggca tactggtttc
     2101 tgccaatcaa atttgctaca gctgaccgac agtgccggtt ttggttccag ttattgggta
     2161 gatcgggtat acctcagcac cgactctacc ggtattaatg gaaacgccat cctcatcgga
     2221 gaaaaaccac aaggagtaat aaacagtgga attcacgcag acaatgcccc ctcttataaa
     2281 tgtgtgcatc cgggaactaa cccggccagt gaaaacatca atacatttaa tgtaataaga
     2341 ccaggttcta accacccagg tgaatttaat tttactatac cggctgacct gctacctgga
     2401 aattattatg tttatgtaag agccaatgcc acacaaacgg tatatgaata tccgggtaca
     2461 cctcaaatca gaagaagtgc attacctatc aatatccgga ggcctgattt agctgtatca
     2521 gctactgtcc ctgcttctgc atctgctgga caacctgtca cgataaccta tagtatcctg
     2581 aataatgggc cgggtactgt attcaatcaa ttacgaaatg acaagatcta tttcagcacc
     2641 tcgcctgttt ttgatggtgc agcccagctt ataaacactc aaagctttac ggaaagcata
     2701 ccggcaggca ccccggttag tcatacattt agttatacca ttccaaacgg agtaagcggt
     2761 aaccgttatt tctatgttca tacaaatttt gacagtactt tccgggaaac caattatagc
     2821 aataatatca gcagtgccgc tcctacgtca gttattaatc ctacaccggc tgacctggtg
     2881 gtaacatctg tgccattggc agattcattg tttaccatct ataattcgaa aataaaatat
     2941 accgttacga ataatggccc cggcagcacc tatggtacat ggactgacag cattttcgtc
     3001 agttgtaacc ctgtgtacag ttcagctaac agttattttg ttgctaaaag gaatcatagt
     3061 ggcactttga atacgggtac ttcttacact gattcagtaa acctaaatat gcagttcagc
     3121 tggacttata atagctgctt tccggaaata gcagcaacaa cagcttattt ctttgtaaaa
     3181 accaatgcat caggtgatgt ttatgaagga gcgaacagca ataataatgt aaatggttct
     3241 ggtgccagaa tacttgtaaa tccattagtt gatcatatag ttacttctat atcaggatca
     3301 gataccgcca cagcagccag gccgtatagc attaactgga cagtaaagaa tattgggcgt
     3361 aatcctggat cagtgtacta tcaattatgg aatgacggtg tatatttctc gccagattct
     3421 ttagaaaatg gaaatgaaat tctggccagc cagtttctga tgactacccg gttggaaagt
     3481 aaccagagct attcagaaac aagacctgca attgttccca atattccgac gggtgattat
     3541 tatgtctttc tgaaaactaa tgtcaccaac tatatccccg gagaaaaaca actgaataac
     3601 aacagcaatt ttatacgaaa tattacaggt gctgccagaa agatacatgt cattcagcca
     3661 ttgctcccgg actttgttga cacaatcttt tctgcacctg ccacagcggc agccgggcaa
     3721 ccagccactg taatacataa aataaccaat tcaggtgcag ggccagttta tcctaataac
     3781 tggagtacca aactctggct ttctacagat tttactcctg gtaatagtgg tgatatagaa
     3841 ctattctccc gcaatatgaa tgctgcgctg cagcctggcc agtcattgaa tgacactatc
     3901 acttttacac tgaacctgaa taccgtaccg ggcaattata tccttattgc acatacagat
     3961 gccaatagct cagtttttga atcaaatgat accaacaatc ttgctttcag ttatgtaaca
     4021 gtattcagga cacagcctgt agatctggtt gttgaaaata taatgaagcc tgatactgtc
     4081 ttgttaggct atagtataga tactgccaaa tgggtcattg ctaacctgtc agccaatacg
     4141 gctacaggag tatcctctga tggaatctat ctttcctcca atacagttct tgattcaact
     4201 gctgtattag ttggaataaa aaacaagaac ataaacatgc caccgcttga cagggacacc
     4261 attagcatgc agccgttggt gaccagcctg gttgaagggc aatacaaggt gctggtgaaa
     4321 acagatttgc tgaataatat taatgaaacg aataagaata ataacacagg tatatcggcg
     4381 ggcagcattt atgtaaaagc aaaggaactg aagttagata taccggaatt gaatacactg
     4441 catacgctga caaggtatta caaactggta ataccggatt cgctgaatgg atctactatc
     4501 cttgtttcac tgaatacaag tgattccctg tccatgcgga atgaaatgta tattggattg
     4561 ggttatgttc cctcttcagc ccgttttgac tacaaattca atacacccaa ttacggtaat
     4621 caacagattg taatgacttc tgttgttgat tcggtgtatt acatcgccgt acgctgtgtg
     4681 tcatcaaatc ctttaattca aaatataaca ctaactgcag tacggcttcc gttcacaatt
     4741 ttaaatgtgc agtccagcac cggtggaaat atcggtaatg taaccgttaa attaagcggg
     4801 tcgttattca ctaataatat gttggcccgt ctcagcaggc cgggtactac tatttactct
     4861 tctgccgttt actttatcaa cagcaccacc gtctttgcca ccttcaacct gcagggaaga
     4921 cctgtagggc tttatgatgt gtcattgcat aagcaagatt catcagtagc agtactagcc
     4981 ggcagcttta caattgccac cggaaataat ggaggcatcc tcaccggagg tggaaataat
     5041 acaggccaga caggaagcgg aaatcagccg ggctgtgatc cgggcacacc aggcggatta
     5101 aactccctgc tggtaacaga agtagtggta cctgaaaaag tatttgtagg ctggccattt
     5161 gtaatacaga tcaactatgc caatcctgcc aattttgata ttccggcaca aacaagaacg
     5221 ctttataatg ataagaatgt gaagatggct atgacacagg cagcccttgc caatggttcc
     5281 aattcattac acctggaact gactgaaccc ggcggtcctc cgggcattat cagggccggt
     5341 gccagcggaa caataactgt ctatggcaaa acaccaccgg atacaccggg tcatacccta
     5401 atcaatttta cactcaggta aaaggcagca aaatgaatcg ttcactttca aannnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagtggg
     5521 tacatctgcc cagaatatca acatacctaa taaaacaggc ccgatgggtt tagaagtaaa
     5581 tacgcttaca ggtaacctct acttcacccg caatgatctg tatattcctg caaggggttt
     5641 cgatttaaat attacttttc attacaatag cttcaattac gacaaaaatg atggttatgg
     5701 taagggatgg agttttaatt ataatataaa atacaaaaac gatacactgg ggagtaaaac
     5761 aataatttgg ggagacggac gggaagataa ttacttacca gaaggagccg gttttaaagc
     5821 accaagggga ttctttagta ccctgagcga ataccagccc ggcaaataca ggattacaga
     5881 aacggatggc acaaagtatt tctttgataa caatatccat aagcgaatca cccggatgga
     5941 agaactcaat ggaaatttta tcaactttaa ctattccgat acacttctta cagcactaac
     6001 aaatggagca ggtcaaacta tttcgcttgc ctatacaaac ggcaggctta ccagcataac
     6061 agatgcaatg actgttccga caagggttat cagctatata tacgatggtg caggcaacct
     6121 ggtggaagta aaagacccgc tgaataatgc aacaaaatat gcttacctcg ttaatggccc
     6181 tatgaaagct gtcaccgaca aaaacaataa taaagttgac ataatctact tcaataattt
     6241 ttccatcagt gaactgatag gatgtaataa aagactgagt ttgtcttatg attctgccac
     6301 ccataccaca atggttactg actacctgga aaccggaagt aaccaggtaa cgaaatatga
     6361 atttaaaaaa caggaggata aaaaatggct tagcggaatg tcagggaact gttgtggttt
     6421 caatatgaaa ttcgaatttg atgccgctgg taataagata aaagaaacgg atgccaatgg
     6481 caatgttacc acatttacgt atgacaaccg gggaaatatg ctcaccatga ctgatgccct
     6541 gaataatact atcaaataca catatacgac agattttaac agggtagcca gttttactga
     6601 tgaaaaaggt tatactacaa caatgacata tgatgtaaaa ggcaatctta cacagttaac
     6661 tgaaccggga aaccatgttt atacagcatc ttatgctgct aatggtgata ttcaaagcag
     6721 cacagacccc aaaggcaatt cgtttagcta taactatgac acctacggaa acccggtaaa
     6781 tgtgaccggc cctaacggat actctgcaca actggtattt gatgcaaggg gaaatcttct
     6841 ttctgtaaca gatgccaggg gaaacacgag taacctggag tatgatatat tgggaaggct
     6901 aaaaaagatt actgacccta tcaataacaa cattcaactt aactatgatg ctgaaggcaa
     6961 ccctgtttcg cttgtaaatg aaaacaatga aaccagcctg tcagcatttg atgccagtaa
     7021 caggctggtt gaatttactg atccgactgg cggaaaaaca acactaacat acgatgcaat
     7081 ggataatata aaaagcatca gaaatacatt aggaaatgca ctgagctttg aacacgatag
     7141 cagaaacagg ttaaaaaaaa taactgatcc tgagggcaat gtactttcct ccgattatga
     7201 tgcaaacggt aatctgactt ccgtaaatat gccgaatggc caggtcttta gttatagcta
     7261 tgatgctctg aacaggatca tagcagcaaa tgatattaac gggcaggtta tcagcctgac
     7321 ctacgataag aacagcaatg tgaccagttt tacgaatggc agcggcgcaa cgtattcagc
     7381 agaatacgac agccttaaca gagttaagaa aattacagac cctttaagca atacatatac
     7441 actactatat gataaaaaca gcaatatagt tgccgtaact gacagaaacg gctttacctc
     7501 cacatatact tatgatagcc ttgaccgggt aaaaaccacg acagataata acggaaatac
     7561 aattacagta aactacgatt tccagggtaa tataactgcg ctgacagatc agaacaataa
     7621 tacgaccagc tacacttatg ataacctgaa ccgacttaaa agaactactt accctgatgg
     7681 caaatacaca gaaaacagct atgataacaa atcaaatatc attgctaaaa gaatcaccga
     7741 tggcactacc attactttta catacgatac actcggcagg gtcatatcaa aaacattgcc
     7801 cgatggccat gtttatactt atacctacga tgcactaggt aggctgctga cagcgaccaa
     7861 taatgccggc actgtaacac taacctatga tgcactgaac cggctgactt ctgaaaattt
     7921 tggcaacaga acagtaaact atacttataa tattgccggc agaacacaat caactattta
     7981 ccccgacagt actgtcatta caaaaagcta tgatacccgc aaccgattga tcagcattat
     8041 caaaaataat tcggtgctgg tatcttacca gtacaacaat gccaaccagc taatagnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnaaa acatccatca acaggctgaa tgatccggcc aggtcggaac
     8281 aattttccta tgataacggc cgcagactaa ctaactacaa aagaggagcc attggcggag
     8341 ttccagttgt agagaacact tataattatg atgcattagg caacaggaca tctgccagtt
     8401 taaatggcac aaatacaacc tacacttcca ataacctgaa ccagcttacc agcagcaata
     8461 acggaactca gaatattaac ttcacctatg acaataatgg caatcttacc tatgacgggc
     8521 agttttataa attttatgat cccgaaaaga agttactgaa agactctgcc tcaccttccc
     8581 aggtttatgt ttaccattat gatgccctca acaggcgatt cagaaaaaat ctgaacggca
     8641 cgaaccgata ttatacattc tcgggcataa cgcctattga agaacgggat gggaccggca
     8701 atacaatcct caataaaaca atttatgcag gctggctgag cccggtggtg aatgaaaaaa
     8761 acaataacca gtttttttat catcagaatg aactgaactc agtggaagcc atcactaacc
     8821 agcaaggaag actgattgaa aaatataatt acgatgcata tggcaaaatg agtatttatg
     8881 attcactgaa taatgcactg cctggctccc ttacaggcaa ccgttttggt tttaccgggc
     8941 aggaatatga cagtacaaca ggcaaataca aatttttctt ccgggaatat aatcctgaaa
     9001 ccggcctgtt cagtcagcgg gacttaattg gttatgcaga cggcatgggt atgtaccagt
     9061 atgtgcataa caacccggcg aatggagtgg atgtcctggg gcttgaagat tgtccacctg
     9121 ataaagcaaa aaatacacag gtaacactaa ggcagtcggt ggaagagctt gaaacactcg
     9181 gatcaaatta tactaccgtt ttgagtttaa taaaccagtt tcggattgaa tttaacattg
     9241 ctaagaatag caaattagat gtgctaatag atggcctgat tgatgcagga gattttgtta
     9301 aagctaatga gcttgcaaan nnnnnnnnnn nnnnnnnnnn nnnnngctaa cgaacttaaa
     9361 gggcttaaag ggctgtcatc aggcttgggc aaacttagct ttggcttaac tggtattgat
     9421 gcattattca aaggcaacac tttgggtaat gttcttaatg atcctaactc cggaactact
     9481 gaaattgcaa aagcttcggg tgatttggca caaagtcttt tgtcattcag cggtataggt
     9541 tcagtatatg gattgtttga ctttgcccaa gagaggttaa taaccggaaa aagcatgaat
     9601 gctctttcag cggaatatgg agaaaatctt ggagataata gcgttgacca aacaatggac
     9661 gaagaagagc aagagtttca tcggaaatta ggtccggagc attttaaaaa atggctaaag
     9721 tttcgcaacc aaactaatat gcgagaagct aggaaattaa gaaatatcaa ttgtccccaa
     9781 aacagttccg gcggcaccca aaaaccggca agtattattg caggtggaat ttccgatatt
     9841 gctgaagcca tctcttcctg ggaccccaac gaaatcatcg ggccggatgg tgtacctgat
     9901 aaaagctggg taagcatcaa tgacaggttg ccttatacgg taacattcga aaatgataag
     9961 tctgcttcag cgcctgccaa gtatgtaaaa gtagtagtgc cggtacatga gaaaatggat
    10021 gctgcaagtt ttcagctcgg tagtttcggt tttaacagtc ttacattcac cgttccgcct
    10081 gctgccgctt cttattacca gcgattggat tgcagggatt cactcggatt atatgtggat
    10141 gtgatagccg ggtatgatgt ggtgaacaat catgccttct gggaatttca atccatcgac
    10201 ccggttacct tcctgccacc tgctgatccg ctaaaaggct tcttattatt acaagatagc
    10261 agtaacctta cctatggaca tggttttgta aacttcagca tcaaaccagt ttcgactgct
    10321 caaactcttg atagtatcct gg
//
LOCUS       DAORLS010000088        10331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3475_length_10331_cov_2.057318, whole genome shotgun sequence.
ACCESSION   DAORLS010000088 DAORLS010000000
VERSION     DAORLS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3475_length_10331_cov_2.057318"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PK977_08280"
     CDS             <1..1038
                     /locus_tag="PK977_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRF18150.1"
                     /translation="KVKLFETEQKVDWATGELMAYGCLLLEGKDVRMSGQDVRRGTFS
                     HRHAVLRDENTDKPYNRLTHIPGATGKFRIYNSLLSEYGVLGFEYGYALANPNALVLW
                     EAQFGDFANGAQTMIDQFISAGEQKWNRMNGVTMLLPHGYEGQGPEHSSARLERFLQL
                     CAELNMVVTNITTAANFFHALRRQLAWPFRKPMINFSPKANLRHMGSYSRVEEFLSGG
                     FKEVIDDANADTNKVKKVLFCSGKIYFDLAERQQKDGGQDIAIIRLEQIYPLPYKQLE
                     ALHKKYKSATWFWVQEEPLNMGAAGFLQMNLKSINFGVVSRQPSASTATGYNKVHMQE
                     QAELIETAFSI"
     gene            1057..>1660
                     /locus_tag="PK977_08285"
     CDS             1057..>1660
                     /locus_tag="PK977_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRF18151.1"
                     /translation="MIDIKVPTVGESISEVTLLKWLKQDGEYVERDEVIAELESEKAT
                     FEVNAERAGVIKLGPLQEGDTLKIGDLLASIDETAAKPETSTKPAEAEKKTASDSRLS
                     TSTAPVINVPNDIRASPVASAIIADKKVDPKTVIPSGPSGKILKDDVLAALSNPGKKA
                     FAGNDLFSRNARTEKMSNLRKTISRRLVESKNTTAMLTTFN"
     assembly_gap    1661..1679
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1680..2291
                     /locus_tag="PK977_08290"
     CDS             <1680..2291
                     /locus_tag="PK977_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRF18152.1"
                     /translation="RIMDIRNKYKDKFKEIHGVGLGFMSFFAKACSVALAEWPSVNAY
                     IDGDQIVYHDYADISIAVSTPRGLTVPVIRNVESLGMADIEKKVVELATKARDSKLTT
                     EELTGGTFTITNGGVFCSLMSTPIINIPQSAILGMHKIQDRAMVVNGQVVVRPMMYLA
                     LSYDHRIIDGRESVSFLVRVKELLENPEQLLIGKDPVRTLLEL"
     gene            2346..3512
                     /locus_tag="PK977_08295"
     CDS             2346..3512
                     /locus_tag="PK977_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fmu (Sun) domain-containing protein"
                     /protein_id="HRF18153.1"
                     /translation="MSIVQSHLNSAVAIIEQYRGEEPLASFLRKYFSRHKKYGSKDRK
                     QIAHLCYCYFRLGKALMNIPTNQRILAALFLCSSTPDLLLDALKPEWSDKVLLPVEEK
                     WAIAEGKNGVSNIFPWSELLSAGLDHLQFCKSLLIQPDLFLRIRPGKTEQVQLKLQQA
                     GIIFSRPDATCIALDNRTKIDELIILDKEAVVQDYNSQKVGDFLSLLPVQNKRRVWDC
                     CAASGGKSIMAVDMLGDIKLTVSDLRESILINLRKRFATAGIKEYKSFVSDLSVKRLP
                     ESDDKFDIVIADVPCSGSGTWGRTPEQLFYFKEEMVEQYARLQKQIMSNVLHHVSPGG
                     YILYITCSVFKAENEEVVNWYLKQNQLNLIKMELLKGYSFKADTMFAALMQKPL"
     gene            complement(3507..>3869)
                     /locus_tag="PK977_08300"
     CDS             complement(3507..>3869)
                     /locus_tag="PK977_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18154.1"
                     /translation="DTASSSYTAVYIDTTTINISTGCIVTSTTNPQTIDESVSIIPNP
                     VTGSSFTLIVETNYVVSNMPVLIYDGKGSLVMQLKETKGTGKKTIDLPAGKLLKGSYF
                     IRVMNGSKIIGTAELLRL"
     assembly_gap    3870..3919
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3920..>4466)
                     /locus_tag="PK977_08305"
     CDS             complement(<3920..>4466)
                     /locus_tag="PK977_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF18155.1"
                     /translation="GDSVLAVGAVNTSGVVGSFSSYGPSGDGQIKPDIASVGVAAMVQ
                     STSNTVITGNGTSYACPKMAGLGTCLWQGFPEYNNMKIVRVLQQSGSKATNPDDRIGY
                     GIPDVKKAFGFLLTEFATSTSTVNGCRVTINWSSKDVGAMKYEIERKAPGETSFSKIA
                     EVNPQAGTLLANRSYQYNNDL"
     assembly_gap    4467..4569
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4570..5132)
                     /locus_tag="PK977_08310"
     CDS             complement(<4570..5132)
                     /locus_tag="PK977_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF18156.1"
                     /translation="MEEIFNPYIAAGRTEQLTADYFNYGTGSYNEIHLHNGEFLHNIG
                     LRGQGMQIGMLDNGFNNYTTLRAFDSVNANGQVLGTWDFVAREANVANDGSHGMSCMS
                     AIAANIPGQFIGTAPKAGFWLYQTEDNASEYPIEEFNWACGVERADSSGADAISSSLG
                     YGYGFDGGIPDYPYSTLDGNTTMAAIAG"
     gene            complement(5180..5530)
                     /locus_tag="PK977_08315"
     CDS             complement(5180..5530)
                     /locus_tag="PK977_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18157.1"
                     /translation="MKKSCFFLFVLVFVAIESNAQFTRYVVRFKNKGGSPYSLSNPSV
                     YLSLRAIERRTRYGIAIDSTDLPVTPSYISQISAIPNVTLLNISKWLNAVTVQTSSSA
                     AITTINALPFVQQE"
     gene            complement(5568..>5789)
                     /locus_tag="PK977_08320"
     CDS             complement(5568..>5789)
                     /locus_tag="PK977_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18158.1"
                     /translation="NVLTVEQIDESLNVPITSPTSYASKSRGVEKYSKNIGLVYKEYE
                     LWEYQPNTGNPAGPYKIGFGVTMWMIDHN"
     assembly_gap    5790..5804
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5805..>6050)
                     /locus_tag="PK977_08325"
     CDS             complement(<5805..>6050)
                     /locus_tag="PK977_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18159.1"
                     /translation="KTYTVTPLSDQVEVFGEDNRRYIKLHMPVKNNFTWKGNKFVPDN
                     TYNPLYPFSNDDNMQDWDFYYDGEPGTFSYRGNNYSNV"
     assembly_gap    6051..6204
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6536..7639)
                     /gene="mnmA"
                     /locus_tag="PK977_08330"
     CDS             complement(6536..7639)
                     /gene="mnmA"
                     /locus_tag="PK977_08330"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRF18160.1"
                     /translation="MSRKGKVLVAMSGGIDSTVTALMLHEQGYEVVGITMKTWDYAAS
                     GGSKKETGCCNLDSFNDARQAAVEHGFAHYILDIREEFGDFVIENFVEEYLAGRTPNP
                     CVLCNTHIKWRALLKRADALNCEFIATGHYAKIRQHENGRYVVSKAIDDTKDQSYVLW
                     GLQQDLLSRTLLPLGPYRKTEIRQMAFDYGYPELAKKHESYEICFVPDNDYRGFLKRR
                     VDGLEEKVAGGDFIDKHGKILGKHKGYPFYTIGQRKGLDIALGKPVFVTKIDPEHNTV
                     MLGDEDDLEQNEMLVGKVNMIKYETITPGIEACTKIRYKDKGALSNLYPENGSMKVRF
                     YEHVKGIAPGQSAVFYEGDDVMGGGIIQRGELV"
     gene            complement(7850..8335)
                     /locus_tag="PK977_08335"
     CDS             complement(7850..8335)
                     /locus_tag="PK977_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18161.1"
                     /translation="MRKQLFAVVMAAALLTSCGSKKAAFQFSQDIVKKERSLITDIEK
                     TEDAVERYNAAEQFDSIAIVGEKMEKLIDEKMQEIKAMKAPKAKGAEEFKSAAVRYFE
                     FMKSLYTGYKNYGKAPSAEERDKVIQDVMDIVNKKDEAIKDMQTAQKKYADDNGFKLE
                     N"
     gene            8453..>8922
                     /locus_tag="PK977_08340"
     CDS             8453..>8922
                     /locus_tag="PK977_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRF18162.1"
                     /translation="MKKILIGFWLLLSVQFTSAQDISYFLPDSVSYNPAIPLPKDVIY
                     HEVGEWHITHDRLVNYMKAIAAAAPDRVKLETMGLTYENRPQVLLIITSKKNHANLEQ
                     IRQQHVQLTDPAKSASLNIEKMPAVVWIGHSIHGNEPSGANASLLSAYYLAAAQG"
     assembly_gap    8923..8940
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8941..>10331
                     /locus_tag="PK977_08345"
     CDS             <8941..>10331
                     /locus_tag="PK977_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRF18163.1"
                     /translation="IDELLENVIILFDPSFNPDGLQRFSTWANQHKSKNLVSDPNSRE
                     FNEVWPGGRFNHYWFDLNRDWLPAVHVESQNRLKWFHLWKPNILTDHHEQGSNATFFF
                     QPGVPSRVNPLTPEKNKDLTAKLGKFHAQFLDRIGSLYFTKEAYDDFYYGKGSTYPDI
                     NGAIGILFEQASSRGHLQETANGLLSFPATIKNQFVTTLSTLEGAKVLRKEFLEYQRS
                     FYKDAAARATAFAVKGFVFGDKNDPAKTFHFAEMLKRHQIQIHTLPDNWNDATFDKSN
                     AYMVSLNQPQHSLVRAIFDKTFEYRDSLFYDITSWTMPLAFGLPYKEITTQGFAMGEM
                     IEEFKLPKHSISGGRSQYAYLLQWEDLYAPAALNELLNAGIMAKVATNDFEMPVGGVM
                     KKFNYGTILIPVKLQRDDIETLFTKLSAVTEKYRIKTFAVSSGNSSSGSDLGSSRFVT
                     ITKPSVAMITGTG"
BASE COUNT         2871 a         2034 c         2151 g         2916 t
ORIGIN      
        1 aaagtgaagt tgtttgaaac agaacaaaag gtagactggg ctacaggaga actgatggct
       61 tatggctgtt tgttattaga aggtaaagat gtgcgaatga gcggacagga tgtgagaaga
      121 ggcacttttt cacacaggca tgcagtattg agagatgaga atacagataa gccatataac
      181 cgcctcactc atatacccgg ggctacagga aaattcagga tatataattc tttgctgagt
      241 gaatatggtg tacttggttt tgagtatggg tatgccctcg caaatcctaa tgcacttgtt
      301 ttatgggagg cacagtttgg cgattttgcc aatggtgcac aaaccatgat cgaccagttc
      361 atcagcgctg gtgagcaaaa gtggaacagg atgaatggtg ttaccatgct gctcccgcat
      421 ggttatgaag ggcaagggcc tgagcatagc agtgcaagat tagaacggtt tctgcagctg
      481 tgtgcagagt tgaacatggt tgttacgaat attacaacag ctgctaactt cttccatgca
      541 ttaagaagac aacttgcatg gcctttccgt aagccaatga ttaatttttc gccaaaagcc
      601 aacctgcgtc atatgggcag ttattcaagg gttgaagaat ttctctccgg tggctttaaa
      661 gaggtaattg acgacgctaa tgcggataca aataaagtaa agaaggtatt attctgcagc
      721 ggtaagattt attttgatct ggctgaaaga caacaaaagg atggagggca ggatattgcg
      781 attatcaggc tggaacagat ttatcctttg ccttataagc aactggaagc attgcacaag
      841 aaatacaaaa gtgctacctg gttttgggta caggaagaac cactaaatat gggagcggca
      901 ggtttcttgc aaatgaatct gaagagtata aattttggtg tggtaagccg ccaaccatcg
      961 gcttctaccg ccacagggta taacaaagta catatgcaag agcaggcaga gctgatagaa
     1021 acagctttta gtatttaact tttgaaacag aaatgtatga ttgatataaa agttcctacg
     1081 gttggtgaat cgatcagcga agtaacattg ctgaaatggt tgaagcaaga tggtgagtat
     1141 gtggaaagag atgaagtaat agcagaactg gaaagtgaga aggcaacctt tgaggtaaat
     1201 gcagagcggg caggtgttat caaactgggg ccacttcagg aaggagatac cctgaagata
     1261 ggcgacctgt tggcaagtat cgatgaaact gctgcaaaac ccgaaaccag tacgaagccg
     1321 gctgaagcag aaaagaaaac agcatccgat agcaggctat caacttcaac agcaccagtt
     1381 attaatgttc ccaatgacat tagagctagt cctgttgcgt cggcaataat tgcagataaa
     1441 aaagttgatc ctaaaacagt aattccttca ggaccttcag gtaaaattct taaagatgat
     1501 gtactggcag ccctttctaa tcccggcaaa aaggcttttg ccggtaatga tctctttagc
     1561 cgtaatgcaa gaactgagaa gatgagtaat ctgcgaaaaa cgatcagccg caggttagtg
     1621 gaatcaaaaa atacaactgc catgcttacc actttcaatg nnnnnnnnnn nnnnnnnnnc
     1681 gaattatgga tatacgtaac aagtataagg acaaattcaa ggagattcat ggagttgggc
     1741 ttggatttat gagctttttt gctaaagcct gttcggtagc tttagcagaa tggccttccg
     1801 taaatgccta tattgacggc gaccagatag tgtatcatga ttatgcagat ataagtatcg
     1861 cagtaagtac acccagggga ttaactgttc ctgtaattcg aaatgtggaa agtcttggca
     1921 tggctgatat cgaaaagaaa gtagttgaac tggctacaaa agcaagagac agcaaactaa
     1981 ctaccgaaga attgacaggt ggtactttca ccattaccaa cggcggtgta ttttgttcac
     2041 taatgagtac gccgatcatc aatataccac agagtgccat tctgggaatg cataagatac
     2101 aggaccgtgc aatggttgtt aatggccagg tggtggttcg gcccatgatg tacctggcat
     2161 taagttatga tcaccgcatc atcgatggca gggagtccgt aagtttcctg gtaagggtaa
     2221 aagaattgct tgaaaatccg gagcagttgt tgattggaaa agatccggtc agaacactac
     2281 ttgagctctg atttttggat ttaattcttc tttgcctata acgtcaagct gacttacttt
     2341 gcagggtgag cattgtacaa tctcatctta attcggctgt tgcaattatt gagcagtaca
     2401 ggggagaaga gccattggct tcttttctgc gaaaatattt ttccaggcat aaaaagtatg
     2461 gatcgaaaga ccgtaagcaa attgcgcatt tgtgttactg ttatttcagg ctgggtaagg
     2521 ccttgatgaa tatacccacc aaccagcgaa ttctggccgc tttgttttta tgcagcagca
     2581 cccctgatct cctcttagat gcgctgaaac cggagtggag cgataaggtt ttgctaccgg
     2641 tggaggaaaa atgggctatt gcagaaggaa agaatggggt gagcaatata tttccatgga
     2701 gtgaactgct gtctgcaggt ctggaccatc tgcaattttg caaatcatta ttaatacagc
     2761 ctgatttgtt tttaagaatt cgaccaggga aaacagaaca ggtgcaatta aagcttcagc
     2821 aggcaggtat tattttcagc agaccggatg caacatgcat cgcattggac aacagaacta
     2881 aaattgatga attgattatt ctggataaag aggcagttgt tcaggattat aattcgcaaa
     2941 aagtcggaga ttttctcagc ttattgcctg tgcaaaacaa aagaagggtg tgggattgct
     3001 gtgcagcaag cggtggtaaa tccatcatgg ctgtggacat gttgggagat ataaagctta
     3061 ctgtttcaga tttaagggaa agtattttga ttaatctcag aaagcgattt gctacagctg
     3121 gtataaagga atacaaaagt tttgtatctg atctctctgt caaaaggctg ccagaaagtg
     3181 atgataaatt tgacattgtt attgcagatg taccttgtag cggtagcgga acatgggggc
     3241 gtacaccaga gcagcttttt tattttaaag aagaaatggt tgaacaatat gcaagattgc
     3301 aaaagcaaat catgtcaaac gttttgcatc atgtaagtcc aggcggatat attttgtata
     3361 tcacctgctc agtatttaaa gctgaaaacg aggaggtggt taattggtat ttgaaacaaa
     3421 accagttaaa tcttattaaa atggaattat tgaagggcta ttctttcaaa gcagacacaa
     3481 tgttcgctgc cttgatgcaa aagcctttat aagcggagta gttctgctgt accaattatt
     3541 ttgcttccgt tcattactcg aataaaataa ctgcccttaa gaagttttcc ggcgggcagg
     3601 tcaattgttt ttttacctgt tcctttcgtt tctttcagtt gcattaccaa tgatccctta
     3661 ccgtcataaa taagaaccgg catattgctt acaacatagt ttgtttctac gattagtgta
     3721 aacgaactac cggtaaccgg gttgggtatt attgaaactg attcatcaat tgtctgaggg
     3781 tttgttgtgc tggtaactat gcagcctgta gaaatattga tagttgttgt atcaatataa
     3841 actgctgtgt aagatgagga agccgtatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnna taagatcatt gttatactgg taactgcggt tggccagtag
     3961 tgtgcctgcc tgcgggttta cttcagctat tttagaaaag cttgtctcac cgggggcctt
     4021 acgttcaatc tcgtatttca tcgcaccgac atctttacta ctccagttaa tagtgactct
     4081 acaaccatta actgttgaag tagaagtggc aaattcagta aggaggaagc caaaagcttt
     4141 ttttacatct ggtatgccat aaccaattct gtcgtccggg ttggttgctt tgcttcctga
     4201 ttgttgtaag acacggacaa ttttcatatt gttatactca gggaatccct gccaaagaca
     4261 ggtgccaagg cctgccattt tcggacaagc gtaagaagtt ccgttgccgg taattactgt
     4321 attagaagtg gattgcacca tggccgccac acccactgag gctatatctg gttttatttg
     4381 cccgtcacca gaagggccat agctggagaa actgcctact acaccgctgg tgtttacggc
     4441 accgacagca agcacactat caccatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnc ctgcaatagc agccatagtt gtattgccat ccaatgtgct gtatgggtaa
     4621 tctggaattc caccatcaaa accatagcca taacccaaag atgaggatat ggcatcagca
     4681 ccggagctat ccgctctttc tacaccacag gcccaattga attcttcaat aggatattcg
     4741 ctggcgttat cttctgtttg atatagccag aaaccggctt tgggagcggt gccaataaat
     4801 tgtcccggaa tatttgcagc aatggctgac atgcaactca taccgtgact gccgtcattg
     4861 gcgacattag cttctctcgc cacaaaatcc catgtgccta acacctggcc gtttgcattt
     4921 acactgtcaa atgctcttaa ggttgtatag ttattgaatc cattatccag cattccgatt
     4981 tgcattccct ggcctcttaa accaatattg tgcaggaatt caccattgtg aagatgaatt
     5041 tcattataag aacctgttcc gtaattgaaa tagtcagctg taagttgttc tgttctgcca
     5101 gcagcaatgt aagggttaaa gatttcttcc aattcaaatt tattcctttc atccgaggga
     5161 cgacccgatg gccgggctgc tactcctgtt gtacaaatgg aagagcattg atagttgtta
     5221 tggctgctga actgctggtt tgcacagtca cggcattcag ccatttagat atattcaata
     5281 aagtcacatt tggtatagca gatatttggg aaatataaga aggggtaacc ggaaggtcag
     5341 tactatctat tgcgattccg taacgggttc ttcgttcaat tgcccttagt gacaaataga
     5401 ctgaaggatt cgaaagcgaa taaggagatc ctcctttatt tttaaatctt accacataac
     5461 gggtaaactg tgcattgctt tcaatcgcta caaatactaa aacaaaaagg aaaaaacagc
     5521 tctttttcat atttaccggc catttcagca cttatcaaaa atacttatta gttgtggtcg
     5581 atcatccaca tagtaacacc aaaacctatc ttgtatggac cggcaggatt accggtatta
     5641 ggctgatatt cccacaactc atattctttg taaacaagac caatattctt agaatatttt
     5701 tcaacacctc tggactttga ggcatacgaa gtggggctgg tgatcgggac atttagtgat
     5761 tcatcgattt gctctactgt cagcacattn nnnnnnnnnn nnnncacatt actgtaatta
     5821 tttccacggt agctaaacgt accgggttct ccatcataat aaaaatccca gtcctgcata
     5881 ttatcatcat tgctgaacgg gtacaacgga ttataggtgt tgtccggaac aaacttgttt
     5941 cctttccagg taaaattgtt ctttaccggc atatgcagtt tgatataccg gcgattgtct
     6001 tcgccaaaaa cttctacctg gtctgaaagg ggagtaaccg tatatgtttt nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnaagacc attgaatcaa gcctgtaagg gatgtatttg
     6241 ccaacagctg ctggccggta gtcgttcaca ttatcggtga caaaggtttc tttttcatca
     6301 tcacatgata caagaaacaa agcagccata gctgccggta atagacggga gaagatcggg
     6361 aatttcataa gcggtttttc cagattcagt gaattaagtt taagatattg acagtattca
     6421 cagggactta gctgccttat gccggcaaca taaacattta aagctacaaa caaaaagcgg
     6481 ggaaacataa tttccccgct ttataacgcc aatttttctt ctttattgcc tgattttaaa
     6541 cgagttcacc tctttgaatg attccgccac ccatcacatc atccccctca taaaatacag
     6601 cactctggcc cggggcaatg ccttttacat gttcataaaa ccggaccttc atggagccat
     6661 tttcagggta caagttggat agagccccct tgtctttata gcggatttta gtacaggctt
     6721 ctattcccgg tgtaatcgtt tcatatttga tcatattaac ctttcctaca agcatttcgt
     6781 tctgttccag gtcatcttca tctcccagca ttacggtgtt gtgttccggg tcaatttttg
     6841 taacaaatac gggtttgcct agtgcaatat caaggccttt ccgttgtccg atagtataga
     6901 acggatagcc tttatgttta cctaaaatct ttccatgctt atcaataaaa tcaccaccag
     6961 ctactttttc ttccaatcca tccacccttc ttttcaggaa accgcggtaa tcattatccg
     7021 gcacaaagca tatttcatag ctctcatgtt ttttagcaag ctcaggataa ccatagtcga
     7081 atgccatttg cctgatctcg gttttgcgat agggccccag tggtaacaat gtccggctca
     7141 gcaggtcctg ctgcaggccc cacagcacat agctttgatc tttggtatca tcaatagctt
     7201 tactaaccac ataacggcca ttttcatgtt gccttatttt ggcatagtga ccagtggcaa
     7261 tgaattcgca attcagggca tcagctcttt tcagcaaagc tctccactta atatgggtat
     7321 tacacagcac acagggattt ggggtgcgac ctgccaggta ttcttccacg aaattctcta
     7381 taacaaaatc gccaaactcc tcccgtatat ccagaatata atgtgcaaag ccatgctcca
     7441 ctgctgcctg tctggcatcg ttgaaggaat cgaggttgca gcatcctgtt tccttcttgc
     7501 tgcccccgct ggcggcatag tcccatgttt tcatagtaat gcccactact tcatagccct
     7561 gctcatgcag catcaatgcc gtaacggtac tatcgatacc gccgctcatc gccactaaaa
     7621 cttttccttt acggctcatg attactaata aaatttagcc tgcaaatata cgtttaacca
     7681 gtgggttgct ttgttacgca aaaggataaa aaacatttaa ggggcaggca aacaatgaca
     7741 gttatggaat gattagcaat ttgttatgga taatttgaag aactgaattc agctaactca
     7801 taaaaaatgc caccggttgg tggcattcat tataaacaca aacaaaaaac tagttttcca
     7861 gtttaaagcc attatcatct gcatactttt tctgtgccgt ttgcatatcc tttatagctt
     7921 cgtccttttt gtttacaata tccattacat cttgtattac cttatctctt tcttcggctg
     7981 atggggcttt accataattt ttatagccgg tataaaggct tttcataaat tcaaaatacc
     8041 ggacagcagc agacttgaat tcttccgccc ctttagcctt tggagctttc atagccttga
     8101 tttcttgcat tttttcatcg atgagctttt ccattttctc gccaacgatt gcgatactgt
     8161 caaattgttc ggcagcatta tatcgctcaa cggcatcttc ggttttttca atatccgtaa
     8221 tcaggcttct ttcttttttt acaatatcct ggctgaactg gaaggcggcc tttttagaac
     8281 cacaggaagt gagtaaagcg gccgccatca ctactgcaaa aagttgcttt cgcataaaca
     8341 ggtttttggt tgataatttt gattaatgtt gggcaacaaa ttaaggatta tttcttgttc
     8401 ggcaacaatt tgctccctat tctgtaattt cctcgttcaa tcacatcact ttatgaagaa
     8461 aattttaatt ggtttctggc tactgttatc ggtacaattt acttcggcac aggatatcag
     8521 ttatttcctg cccgattcgg ttagttataa tcccgctatc cctctgccta aagacgttat
     8581 ttatcatgag gtaggtgagt ggcatattac ccacgacagg ctggtcaatt atatgaaagc
     8641 cattgctgct gctgctcccg atcgggtaaa acttgaaacg atggggctta cctatgaaaa
     8701 tcgtccacag gttttactga tcattacatc taaaaaaaat catgcaaacc tcgagcagat
     8761 caggcagcaa catgtacagc tgactgaccc ggcaaaatca gcttcactga atattgaaaa
     8821 aatgcctgct gtcgtctgga taggccactc cattcacggt aatgaaccca gcggtgctaa
     8881 tgcctcattg cttagtgctt attacttagc tgccgcacaa ggnnnnnnnn nnnnnnnnnn
     8941 agattgatga gttgcttgaa aatgtgatta tcctttttga tccttcattt aatcctgacg
     9001 gcttgcagcg tttctctacc tgggcaaacc agcacaagag taaaaacctg gtaagcgatc
     9061 ccaattcaag ggagtttaat gaggtatggc ccggcggccg tttcaatcat tattggttcg
     9121 acctgaaccg tgactggtta ccggcagtgc atgtcgaaag ccagaaccgg ttgaaatggt
     9181 ttcatctctg gaaaccgaat atccttactg atcatcatga gcagggtagt aatgctactt
     9241 tcttttttca gccgggtgtt ccttcaaggg tcaacccatt aaccccggaa aaaaataaag
     9301 acctaacggc caagctggga aaatttcatg cgcagttcct tgatcgtatc gggtctttgt
     9361 atttcaccaa agaagcctat gatgattttt attatggcaa aggatccacc tacccggata
     9421 ttaatggcgc tattggtatt ttgtttgagc aggcttcttc cagaggccat ttgcaggaaa
     9481 cggccaacgg cttgctgagt tttccggcga ctatcaaaaa tcaatttgta actaccttat
     9541 ctactctcga aggggctaaa gtattacgaa aggaatttct cgaatatcaa agaagtttct
     9601 ataaagatgc tgctgccagg gccactgcct ttgctgtcaa aggatttgtt tttggtgata
     9661 agaatgaccc ggcaaaaacg tttcattttg ctgaaatgct gaaacggcac cagatacaga
     9721 tacatacatt gcccgataat tggaacgatg caacttttga taaaagcaat gcctatatgg
     9781 tttcgcttaa ccagcctcag cacagtctgg taagggccat ttttgataaa acattcgagt
     9841 acagggacag tttgttctat gatattacat catggactat gcccctggct tttggtctgc
     9901 cctataaaga aattacaaca cagggatttg ctatgggtga aatgattgaa gaatttaaac
     9961 tgccaaaaca ctctatcagc ggaggccgca gccagtatgc ctatttatta caatgggaag
    10021 atctgtatgc gccagcggcc ttgaatgagt tgttgaatgc tgggatcatg gcaaaagtag
    10081 ctaccaatga ttttgaaatg ccagttgggg gtgttatgaa aaagtttaat tatggtacaa
    10141 tacttattcc ggttaagctt caaagagatg acatcgaaac gctgtttacc aaattgagtg
    10201 cagttacaga aaagtatcgt atcaaaacat ttgccgtaag cagtggcaat tcatcaagcg
    10261 gaagtgatct gggcagtagc cggtttgtta ccattaccaa accatctgtg gcaatgatta
    10321 ccggtacagg a
//
LOCUS       DAORLS010000089        10167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3543_length_10167_cov_1.698378, whole genome shotgun sequence.
ACCESSION   DAORLS010000089 DAORLS010000000
VERSION     DAORLS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3543_length_10167_cov_1.698378"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK977_08350"
     CDS             <1..969
                     /locus_tag="PK977_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF18164.1"
                     /translation="KVLAYSTVSQLGFMFLALGTGAYVAAVFHVMTHAFFKALLFLGS
                     GSVIHAMSGEQDIRNMGGLSKKLKITFITFLVGCIAIAGIPPFSGFFSKDAILLSAYI
                     KSPLLYGIALFAALLTAFYMFRLLFITFTGTFRGTDDQKHHLHESPVSMTIPLVVLAI
                     LSIIGGFVGIPEAIMHGGDKLTAFLSPVIAQHGEHTVSHTTEYMLMGLSTALVVVMII
                     AAWFVFRNYQRSEATGFGKVLENKWYVDELYEAAFVKPLKRFGSFLNNVFEKSVVDGL
                     VNGVGKAVNYSSRQLRLLQSGQVGSYVLLMVLSMVVFFVIQFFIRK"
     assembly_gap    1104..1338
                     /estimated_length=235
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1339..>1943
                     /locus_tag="PK977_08355"
     CDS             <1339..>1943
                     /locus_tag="PK977_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF18165.1"
                     /translation="MGVFLAMDALLFYFFWELALIPMYFLCSQWGGEKRIAVTIKFFI
                     YTFVGSLLMLIAILFVYSKTTDRSFSLQSFYDVAVTLNSKQQNLVFWLMFAAFAIKMP
                     IFPLHTWQPDTYEQAPTATTMALSGVMVKMGVFGIIRWLFPIVSGASFQWGDFITPMA
                     IIGMIYASLIALRQDDLKRLVAYSSIAHIGLLCAGLFTQNT"
     assembly_gap    1944..1989
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1990..2483
                     /locus_tag="PK977_08360"
     CDS             <1990..2483
                     /locus_tag="PK977_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF18166.1"
                     /translation="INIIGLWIVVEWIERNTGTRKISELGGLAQTAPALAIFFVIIAL
                     ANIALPLTNAFVGEFLIFNGILSSYTQYYVWFTVMAGVCIILAAAYTLRMVRKVAYGN
                     VNDITSKVTDIRFNEKLALGIIVILIFWIGVFPQPMLDLTNGVTESIVNKFEVIRYPT
                     VPK"
     assembly_gap    2561..2599
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2600..3898
                     /locus_tag="PK977_08365"
     CDS             <2600..3898
                     /locus_tag="PK977_08365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRF18167.1"
                     /translation="IRNVAIGGMVLLIVVNLLEFSGIKFFEINTRGMIAFERFALVFN
                     TIIFASTLIYFLLSARDMEKVGVNYAEYFALIFFILCGVTLTSSFKSLLILFLGIEII
                     SIPLYILTGSDKRNLKSNEASLKYFLMGSFSTGIMLMGIALVYGAKGTFDTTAIDSYS
                     QAAPSALLLGGMLLLIFSMGFKVSVAPFHFWTPDVYDGAPTVFTSFMATIVKAAVFIG
                     FIRLFDQTFGNLQAQWQLFFAVITAVTLFIGNITAVFQQSVKRMLAYSSISQAGFMLL
                     AVYAMNTDAKEGLLLYTAAYSLATIGIFAVLIKMSDYTFEGFNGLAKHQPLLAATTTI
                     FLLSLAGIPLTAGFLSKFYMLKAALATGKNLWLVIFAVLMAAVSVYYYFRVIQAMYFK
                     DGEPQSIQVTSAFKWTLVALAALVILLGIYPNLIIGWLYF"
     gene            3979..>4789
                     /locus_tag="PK977_08370"
     CDS             3979..>4789
                     /locus_tag="PK977_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18168.1"
                     /translation="MNFTDIFSLAFRTIRGNKLRTGLTVAIIAFGIMALVGIITAIKA
                     MNQKFTESFSTMGANSFTVRFKERQIRFGGGGNQDVKLTKKGGRKEKKSNLGKLITQD
                     EAELFVQRFNFPSVKSISLFGNRNNIVSHENRKTSPNVMMFGGDENYLMLNGFNLLAG
                     RNINRLDVQTSRNVCILGYDVAKKLFKQGADRAVNAVVRINNVPYRVLGVLESRGSTF
                     GFSRDNVIITSYKNVDRNFPSGFSYVVAVMTNDLLKVNEAMGEAEGIFRAVR"
     assembly_gap    4790..4802
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4803..5258
                     /locus_tag="PK977_08375"
     CDS             <4803..5258
                     /locus_tag="PK977_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF18169.1"
                     /translation="TEESNFVLDRSDSVAEKAMNSLRFLTVSATVIGLITLIGAAIGL
                     MNIMLVSVSERTREVGLVKAIGGKSSMVSRQFLLEAIIISLLGALLGIVLGIMVGNLF
                     SIVLKTGFVVPWNWVIYGIMLCTAVGLLAGLYPALKAGKLNPIEALRYE"
     gene            5481..6341
                     /locus_tag="PK977_08380"
     CDS             5481..6341
                     /locus_tag="PK977_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRF18170.1"
                     /translation="MAEIKPTATASVKATSVQPKKSSNAISWIAPVVCIIGGYAIWRF
                     MMGNPSGFEQPDASGGFWPHHLKPKDAFHRIYEGGIIVPLLIGMFLMVIVFSIERFLT
                     IRKALGKGSISDFIRKVQYHLANRNVDAALSECDKQRGSVGNVMKAGLRRYKEMINEG
                     NLNTDQKVIAIQKEVEEATALELPMLQKNLVFLSTITSVGTLIALLGTVMGMIRSFAA
                     LGEEGGAGAAADLAVGISEALYNTALGIGTSAIALIMYNVFTTKIDSITYGIDESGFT
                     LTQSFASLYK"
     gene            6403..>6532
                     /locus_tag="PK977_08385"
     CDS             6403..>6532
                     /locus_tag="PK977_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF18171.1"
                     /translation="MPSVKIPRKSTDTDMTPFVDIAFLILSFFIMATKFKPPEPVEI"
     assembly_gap    6533..6556
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6602..>6883
                     /locus_tag="PK977_08390"
     CDS             6602..>6883
                     /locus_tag="PK977_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF18172.1"
                     /translation="MVIIDSSNKVYFTVLSDKDKSKFDAIIENINTTRNLGLTPAEKA
                     NYRQTFAVGVPFGNLKQLLATPADQQKSIKQPGIPVMDSANNELVWWIAA"
     assembly_gap    6884..6946
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7222..>7269
                     /locus_tag="PK977_08395"
     CDS             7222..>7269
                     /locus_tag="PK977_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18173.1"
                     /translation="MVDLGFLLITFFVFTA"
     assembly_gap    7270..7279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7280..7766
                     /locus_tag="PK977_08400"
     CDS             <7280..7766
                     /locus_tag="PK977_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF18174.1"
                     /translation="TTPTTMDLNMPKDVKDEKQQTEAKESSVLTIMLGKANQVYYYEG
                     KLVVDATGNNFKSTTFKGIREVIINKKKDVISRHQHDETCKKTQDKAKEKGDPDWEKA
                     CLDRDFIVVIKPDADATYSNTVDILDEMTINQVKTYAMVKILPQEVDLIKATEATNGI
                     K"
     gene            7825..>8335
                     /locus_tag="PK977_08405"
     CDS             7825..>8335
                     /locus_tag="PK977_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF18175.1"
                     /translation="MEANKILSADILDLIFEDRNKDYGAYELRKTYNRRIPRALIITA
                     SIAALALAGSVLSSSLKSKKDGKVKMQEVTLQDIKQEEEKKVVPPPPPPPKVEPPKVE
                     MKQFTPPVIKKDEEVEKPPPPQEELKDVKIAEIDQEGIKDDNIATPVAIDEGKQIVEE
                     KKEDPDENKI"
     assembly_gap    8336..8360
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8361..8657
                     /locus_tag="PK977_08410"
     CDS             <8361..8657
                     /locus_tag="PK977_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF18176.1"
                     /translation="AFPGGDSKWRRYLETNCNGQVATDNGAPEGTYTTIIQFVVDKEG
                     NISDVRPMTNHGYGMEEEAMRVIKKGPKWTPAVQNGRSVKAYRKQPITFQVLGE"
     gene            8746..10128
                     /gene="mnmE"
                     /locus_tag="PK977_08415"
     CDS             8746..10128
                     /gene="mnmE"
                     /locus_tag="PK977_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRF18177.1"
                     /translation="MMTKLSGWDDTIVAIATPQGVGAIGVIRLSGNKAFEIINHLFPS
                     KDLDHQPTHTLHVGYLKEGDKVLDEVVVSLFKAPKSYTGEDVIEISAHGSPFIHQQIV
                     GACVKEGARLAKPGEFTQRAFLNGKLDLAQAEAVADLIASNTAASQKAALHTIRGGFS
                     ANLKDLRDRLLKFSALIELELDFSQEDVEFADRKQFNELINEIQSSTGKLINSFQLGN
                     VIRNGVSVAIVGKPNAGKSTLLNNLLNENRAIVSDIPGTTRDTIEEVINIDGILFRLI
                     DTAGIREHTTDVIESAGIERSLEKIKQADLVLYVFDINEGIAELKTWQLQAEKDGVRF
                     LLVANKIDSADEKVAREKFAAFDHIVFISAKNNMHTEVLKEKMVDMILEGKVQPENTI
                     ITNARHFHALKEVARSLNDIKEGLDKKIPGDLLALDIRRSLHYLGEITGEITNEDQLD
                     YIFSKFCIGK"
BASE COUNT         2821 a         1749 c         2209 g         2933 t
ORIGIN      
        1 aaagtattgg cttattctac tgtaagccag ttaggtttta tgtttcttgc gttaggtaca
       61 ggggcttatg tggcggctgt tttccatgtg atgacacatg cctttttcaa agcattactg
      121 ttcctgggta gtggcagtgt tattcatgcc atgagcggag aacaggatat ccgtaatatg
      181 ggagggttga gtaaaaagct taaaattact tttattactt tcctggtcgg ttgtattgct
      241 attgccggca ttccgccttt tagcggattc ttttcaaagg atgcgatctt attgagtgca
      301 tatatcaaaa gtcccctgct atatggaata gcactgtttg ctgctttgtt gacagctttc
      361 tatatgttcc gtttgttgtt catcactttc actggtacat tcaggggaac tgatgatcag
      421 aagcatcatt tgcatgaaag tcctgtatca atgacgattc cactggttgt attagccata
      481 ctatctataa ttggcggctt tgtaggtatt ccggaagcaa ttatgcacgg gggagataaa
      541 ctcacagctt ttctttctcc tgttattgct cagcatggtg aacatactgt ttctcataca
      601 acggagtaca tgctgatggg attatcaact gctttagtag ttgtaatgat tatagcagcc
      661 tggtttgtat ttagaaacta tcagcgtagc gaagcaactg gatttgggaa agtactggag
      721 aataaatggt atgtggatga gctatatgaa gcggcctttg taaaaccgtt aaagagattt
      781 ggttcatttt taaataatgt atttgagaaa tctgtggtag atgggttggt gaacggggta
      841 ggaaaggcag tgaactacag cagccgccag ttacgtctgt tgcagagtgg ccaggtaggc
      901 agctatgtgc tgctgatggt attaagtatg gtggtgtttt ttgtcatcca gttttttatt
      961 agaaaataac cgggtttatt agaaatagat tatgattcca gttttattga tattggttcc
     1021 gttgctgacc ggcttagctg ctttcattct gaagaatgag aaaacggttc gctcctgggc
     1081 attattttca tcattgataa catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnat gggtgtgttt ttggcaatgg atgcgttgtt gttttacttc
     1381 ttctgggaat tggcgcttat ccccatgtat tttctctgtt cacaatgggg tggtgaaaaa
     1441 agaattgccg ttacgatcaa attcttcatt tatacatttg ttggctcatt gctgatgctg
     1501 atcgccattt tatttgtgta cagcaaaaca actgacaggt cttttagtct tcagtcattt
     1561 tatgatgttg ctgttacatt gaacagtaag caacagaacc tggtattctg gctcatgttt
     1621 gcagcatttg caatcaaaat gcccattttt ccgctgcata cgtggcaacc cgatacttac
     1681 gaacaggcgc ctactgccac tactatggcc ctgagtggtg tgatggtgaa aatgggggtt
     1741 tttggaatta tccgctggtt attccccatt gtttccgggg cctcttttca gtggggagat
     1801 ttcatcactc caatggcaat cataggtatg atctatgctt cactgattgc ccttcgacag
     1861 gatgatctga agaggttagt cgcttattca tcaattgccc atataggttt attatgcgca
     1921 ggtttgttta cacaaaatac aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnng cattaatatc attggcttgt ggatcgtggt ggaatggatt gaaagaaata
     2041 cgggcacccg taagatatct gaattgggag gtttggcaca aaccgctcct gccctggcca
     2101 ttttctttgt gatcattgcc ctggccaata ttgccttgcc actgacaaac gcttttgttg
     2161 gggagttcct gatttttaat ggcattctta gttcgtatac acaatactat gtttggttta
     2221 cggtgatggc tggtgtctgt ataatattag ctgctgcgta tactttgcga atggtaagaa
     2281 aagtggccta tgggaatgta aatgacatta cgtcaaaagt gacagatatc cggttcaatg
     2341 aaaaactggc attagggata attgtgatcc tgattttctg gataggagta tttccgcagc
     2401 caatgcttga cctgaccaac ggggtgacag aatcgattgt aaacaagttt gaagtaataa
     2461 ggtatcccac agtacctaag tgataaataa agtatgaacg cattaatatt atcggctatt
     2521 ctcggggtta tgatgatgtt cggcagtttt ctgctgaaat nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnna ttcgtaatgt agccattggt ggtatggttc ttttgatcgt
     2641 agtaaacctg ctggaatttt cagggattaa gttttttgag atcaataccc ggggaatgat
     2701 cgcttttgaa cggtttgccc tggtatttaa cactattatt tttgcttcta cattgattta
     2761 tttcctgcta tcagcaaggg atatggaaaa agtaggggta aattatgctg aatattttgc
     2821 gctgatattc ttcatacttt gtggtgtaac attaacatcc tcatttaaat cactgctgat
     2881 attgtttctc ggaattgaaa ttatttccat tccgctctat atccttacag gtagtgacaa
     2941 gagaaactta aaaagtaatg aagcctcatt gaaatatttc ctgatgggct cgttttctac
     3001 cggaattatg ctcatgggta ttgcattggt atatggagct aagggcactt ttgatacaac
     3061 tgcaattgat tcgtattcac aggcagcccc ttcggcgttg ctccttggcg gaatgctcct
     3121 gttgattttt tccatggggt tcaaggtttc ggtagcaccc tttcatttct ggacaccaga
     3181 tgtgtatgac ggggctccta ccgtattcac ttcgttcatg gctactattg taaaagcagc
     3241 cgttttcatt ggatttatcc gtttgttcga tcagaccttt ggtaatcttc aggcacaatg
     3301 gcagctcttc tttgcagtaa taactgctgt tactctgttt attggaaata tcacagcggt
     3361 attccagcag agtgtaaaaa gaatgctggc atattccagt atttcccagg ctggttttat
     3421 gttgctggct gtatatgcca tgaatacaga tgccaaggaa ggtttattgc tttatacagc
     3481 tgcttatagc ctggccacaa ttggcatttt tgcagttctc atcaagatga gcgattacac
     3541 atttgaaggt ttcaacggcc ttgcaaagca tcaaccgctg ctagcagcaa ctactaccat
     3601 cttcctgctt tcattggcgg gtattccgct tactgcaggg tttctgtcaa agttctatat
     3661 gcttaaagct gccctggcaa ccggtaagaa cctttggttg gtaatcttcg cggtactgat
     3721 ggctgcagtt agtgtttatt attatttccg ggttatacag gccatgtatt tcaaagatgg
     3781 cgaaccgcaa tctatacaag taacatcagc ctttaaatgg accctggttg cactggctgc
     3841 tttggtgata ttgctgggta tttaccctaa tcttattatt ggctggctat atttctaaga
     3901 aaaggaaata ccagctacct ccacctgtca aaaaacagtt tttctacggc ttaaaggatt
     3961 tacctttgaa aggccgttat gaattttaca gatatatttt ctctagcatt ccgcactatc
     4021 aggggaaata agctcagaac agggcttacg gtggcaatca ttgctttcgg catcatggcg
     4081 ttggtaggta tcatcactgc tataaaagcc atgaaccaga aatttactga gagtttttct
     4141 acaatgggtg ccaatagttt tacagttcga tttaaggaaa gacaaattcg ttttggcggc
     4201 ggcggcaacc aggatgtaaa gctcaccaag aaaggtggcc gcaaagaaaa aaaatcaaat
     4261 cttggcaagc ttattacaca agatgaagct gagctatttg ttcagcgatt caattttcct
     4321 tcagtaaaaa gtatctctct ttttggtaat cgcaataata tcgtttccca tgagaacaga
     4381 aagacaagtc ccaatgtaat gatgttcgga ggtgatgaga attatttaat gctgaatgga
     4441 ttcaaccttt tggcaggaag aaatatcaac aggctggatg tgcaaacgag ccgcaatgtt
     4501 tgtatacttg gttatgacgt agcaaagaaa ttattcaagc agggagcaga tagagctgtt
     4561 aatgctgttg tcaggattaa taacgttcct tatcgggtgt tgggtgtgct ggagagcagg
     4621 ggttctacat ttggctttag ccgggataat gtgattatta cttcctataa gaatgttgac
     4681 agaaattttc cttctggttt ttcatatgta gttgctgtta tgaccaatga cctgctgaaa
     4741 gtaaatgaag ctatggggga ggctgagggt attttcaggg cggttcgtan nnnnnnnnnn
     4801 nnaccgaaga gagcaatttt gtgctggacc ggagtgatag tgttgctgaa aaagcaatga
     4861 atagcctgcg gtttcttacg gtttcagcta cggttatagg tcttataaca ctgattggtg
     4921 ctgccatcgg gctcatgaat attatgcttg tatctgtatc ggaaagaacc cgggaggttg
     4981 gcctggtaaa agcaataggg ggcaaaagca gcatggtaag ccggcagttt ttactggagg
     5041 cgatcattat tagtttactg ggggcattgc tgggcatcgt cttaggaatc atggtcggaa
     5101 acctattttc tatagtgctt aaaacaggct ttgtggtgcc ctggaactgg gttatctatg
     5161 gcataatgtt atgcacggct gtcggtttat tggccggact ttatcctgct ttaaaagcag
     5221 gaaagctcaa cccgattgaa gccctgcgct acgaataaaa aagcacaaaa ttgaccagca
     5281 tccagttcta aaaaaaagag gtgaaattct catcaaattc cctgattcta agttgttccg
     5341 tttgaaaaaa tccttaactt gacccccctt accaaaaaaa tgaggaaagg agaacattgt
     5401 ttaaactttt ttcttagcat tgtacgactg aaaaacggat tagtacattt cattaaaaac
     5461 aaaaattaaa aattaagatc atggcagaga ttaaaccaac tgcaacggct tcagttaagg
     5521 ccacatcggt tcaacccaaa aaaagcagta acgctatttc atggattgcg cctgtcgttt
     5581 gtattattgg cggttatgct atttggcgtt ttatgatggg taatcctagc ggatttgaac
     5641 aaccagatgc atctggtgga ttctggccgc atcacttaaa gccaaaagat gccttccaca
     5701 gaatttatga gggcggtatc attgtaccgt tgttgattgg aatgttcctg atggtaattg
     5761 ttttctcaat tgaaaggttt ttaactatca gaaaagcatt aggtaaaggt tctatttctg
     5821 attttatcag aaaagtacag taccacctgg caaatcgtaa tgttgatgca gctttatctg
     5881 agtgtgacaa acaaagaggt tctgtaggta atgtaatgaa agcaggactt cgccgctaca
     5941 aggaaatgat caacgaaggc aatctgaaca ctgatcagaa agttattgct attcaaaaag
     6001 aagtagaaga agcaactgca ttggaattgc caatgttgca aaagaacctt gtatttcttt
     6061 ctactattac ttcagttggt acactgatcg ccctgttagg tacggtaatg ggtatgatcc
     6121 gttcattcgc tgccttgggt gaagaaggtg gtgctggtgc tgctgctgac ctggcggttg
     6181 gtatctctga ggccctctat aatacggctt taggtatcgg tacttctgcg attgccctta
     6241 tcatgtataa cgttttcact accaagatcg actcaataac ttacggcatt gatgagtctg
     6301 gttttacact tacacaaagc tttgcttctc tttacaaata agaggatctt ttttgtggaa
     6361 aaatgaaccc tattcatctc atttctaaaa tatttgtaaa caatgcctag tgttaaaata
     6421 ccaaggaaga gtacagatac ggatatgaca ccttttgtgg acatagcctt cctgattctc
     6481 tctttcttta tcatggcgac caaattcaag ccacctgagc cggtagaaat tannnnnnnn
     6541 nnnnnnnnnn nnnnnnccgg gatcggtatt gtctcaaaaa ttgcctgaga ataacgctgt
     6601 tatggtaata atagactctt caaataaggt ttattttacc gtattatctg ataaggataa
     6661 aagcaagttt gatgctatta ttgagaatat caatactacc cggaatctgg gtttaactcc
     6721 tgcagaaaag gctaattaca ggcaaacttt tgcagtaggt gtgcctttcg gcaaccttaa
     6781 gcagctgctt gcaacgcctg cagaccagca aaaatcaatt aagcaacccg gcataccagt
     6841 tatggactct gccaataatg aactggtttg gtggattgcg gctnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg tgatggacac
     6961 tctaagtatc ctgcttttga agctgtagtt agtgccttga agcgcaatga tgaattcaaa
     7021 tataacttag taacagctct ggatgacatt ccgtccaatt ctgaattgga cttgtataac
     7081 aggagagtta ctaaataatt taaaaaacaa aatcagtaaa tcatggcaag tatagatacc
     7141 ggtggtggcg atagtggcca caaaaaagga ccaggggtaa agaaggccaa aaaactttct
     7201 acccgggtag acatgacgcc gatggtggat cttggcttcc tgctgattac gttcttcgtt
     7261 tttacggcan nnnnnnnnng acgactccta caacaatgga tctgaatatg ccgaaagatg
     7321 ttaaagacga aaaacagcag acagaggcta aagaatccag cgtactgacc attatgcttg
     7381 gaaaagcaaa ccaggtgtat tactatgaag gaaaactcgt tgtggatgca actggtaata
     7441 attttaaatc aacaacattc aagggaatcc gtgaggtgat catcaataaa aagaaagatg
     7501 tgattagccg tcaccagcat gatgagacct gtaaaaaaac acaggataaa gccaaagaaa
     7561 aaggagatcc tgattgggaa aaggcttgtc ttgacaggga tttcatcgtt gtgatcaagc
     7621 ccgatgcaga tgctacttac agcaatactg tagatatact tgatgaaatg accattaacc
     7681 aggtgaaaac ctatgcaatg gttaagatat taccacagga agttgatttg ataaaagcta
     7741 cagaagcaac taatggtata aaatagtttg ccggcagtta tggaaagaca aagctgtgtg
     7801 catctaataa aaaacgttta actcatggaa gccaacaaga ttttatccgc cgacattctt
     7861 gacctgatct ttgaagacag gaacaaggac tatggtgctt atgagttgag aaaaacttat
     7921 aaccgccgca ttccccgggc tttaattatc actgcatcta ttgctgctct cgccttagcc
     7981 ggatcagttc tttccagctc tcttaaatcc aagaaagatg gtaaagtaaa aatgcaggaa
     8041 gttacccttc aggatatcaa gcaggaagaa gagaagaaag ttgttcctcc tcctcctcct
     8101 cctcctaaag tggaaccccc gaaagtagaa atgaaacagt ttactcctcc tgtgatcaaa
     8161 aaggacgaag aggttgaaaa accccctcca ccccaggaag aattgaaaga tgtaaagatc
     8221 gctgagatcg accaggaagg tattaaagat gataatattg ccactcctgt agcaattgat
     8281 gaaggaaagc agatcgtgga agagaaaaaa gaagatccgg atgagaacaa gatctnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn gcctttccgg gtggtgatag caaatggcgt cgttatcttg
     8401 aaacaaactg taatggacag gtagctacag ataatggagc accggagggc acttacacca
     8461 ccattatcca gttcgtggtt gacaaagaag gtaatatcag tgatgtgaga cctatgacca
     8521 atcacggtta tggaatggaa gaagaggcaa tgagggttat taagaaaggt cccaaatgga
     8581 cacctgccgt tcagaatggt cgttcagtaa aagcctatcg taagcaacct attacattcc
     8641 aggttttggg tgaataagta taatctgatt caataaaaat cctttctggt aacagaaagg
     8701 attttttgtt ttcgggtgtt aagtaatcaa atctaatttt gtggaatgat gaccaaacta
     8761 tcgggttggg atgataccat tgtggcaata gccactccac agggtgtggg tgcaataggt
     8821 gtaatcaggt taagcggtaa caaagctttt gagattatta atcacttgtt tccgtcaaaa
     8881 gacctggatc atcaacccac acatacatta catgtagggt atctgaagga gggggataag
     8941 gtgctcgatg aggttgtagt gtctcttttt aaagccccaa aatcttatac tggtgaagat
     9001 gtaatagaga taagtgctca tggttcacct tttattcatc agcaaatagt gggggcctgt
     9061 gtaaaagaag gagccaggtt ggcaaaaccg ggggagttta cacaacgggc atttcttaac
     9121 ggaaaactgg atctggccca ggctgaagcc gtagctgacc tgattgcatc taatacagca
     9181 gcctctcaaa aagcagccct ccatactatt cggggtggtt tttcagctaa tctaaaggat
     9241 ttgagagata ggttgcttaa gttttctgcc ttaattgaac tggaacttga cttctcccag
     9301 gaggatgtag agtttgctga tcgcaagcag ttcaatgaat taattaatga aattcaatct
     9361 tctactggca aactgatcaa ttcttttcag ttagggaatg tgataagaaa tggtgtaagt
     9421 gtagccattg taggtaaacc caatgctggg aaatctactt tgcttaataa tcttctcaat
     9481 gagaacaggg caattgtcag tgatataccc ggaacaacaa gggatactat agaagaggtt
     9541 ataaatattg acggaatcct tttcaggctt attgatactg cgggtatcag ggagcacaca
     9601 actgatgtta ttgaatcagc aggtattgaa agaagccttg aaaaaattaa gcaagctgac
     9661 cttgttcttt atgtatttga tataaatgaa gggattgctg aactaaagac gtggcagctg
     9721 caggcagaaa aggatggggt aagattctta ttagtggcca acaaaattga cagtgctgac
     9781 gaaaaagtag cccgggaaaa attcgcagcc tttgaccaca ttgtttttat ctctgcaaag
     9841 aataatatgc acactgaggt attgaaggaa aagatggttg atatgatttt ggaaggaaag
     9901 gttcaacctg agaatacaat tatcaccaat gcgaggcact ttcatgcttt aaaagaagtg
     9961 gcccgttcat taaatgatat taaagaaggg cttgacaaaa aaataccagg agacttactg
    10021 gcattagata tcaggcgtag cctccattat cttggcgaga taacagggga gataaccaat
    10081 gaagatcagt tggattatat tttttccaaa ttttgtattg gaaagtaatt atctttcctt
    10141 tgtttgttaa gtacctgttt ttcatag
//
LOCUS       DAORLS010000090        10156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3547_length_10156_cov_2.081576, whole genome shotgun sequence.
ACCESSION   DAORLS010000090 DAORLS010000000
VERSION     DAORLS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_10156_cov_2.081576"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..>1387)
                     /locus_tag="PK977_08420"
     CDS             complement(138..>1387)
                     /locus_tag="PK977_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF18178.1"
                     /translation="MPKLLQAAGYQTAIIGKWHLHSYPTGFDYWKILPGQGLYVNPRL
                     IEMSGDTITQQGYATDVITDETIKWLERRDKSKPFLLLLHHKAPHRYFFPNLKHLEAY
                     HNKTFPEPSTLYVDTFNRGSAWRMQTMSILRDMELCSDLKVDPAFLIDIPEYKPDSAQ
                     IAYYKAIMNRVPEEQRKRYKEIYAERGKLLRELRPKGNELLKYKYQWYMQDFLACVAS
                     IDESVGRILDYLDKEKLASNTAVMYTSDQGFFLGENGWFDKRFAYDVSMQVPLLMKWP
                     GQIKTGTTINTMVQNIDYAPTILDIAGEKIPGWMQGVSLKPLVTGKQKTLNRNYLYYH
                     YYEYVRDHTVIPHLAVRGNRYKLIYFYTVNEWELYDLKNDPQEQKNLVNSPAHQKIRQ
                     QIKNELLKLRDKYDDHEKAGELN"
     assembly_gap    1388..1444
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1445..1702)
                     /locus_tag="PK977_08425"
     CDS             complement(<1445..1702)
                     /locus_tag="PK977_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF18179.1"
                     /translation="MQKLLSLLLLASGSLVAQQRPNIIYIMSDDHDADAISAYNKTLI
                     QTPNIDRLVKEGVKFTNAFVGNSICGPARATLLTGQHSHKNG"
     gene            complement(1734..2471)
                     /locus_tag="PK977_08430"
     CDS             complement(1734..2471)
                     /locus_tag="PK977_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRF18180.1"
                     /translation="MCYDMSFFSNIRLISDYLQIPASDSISFTPTYHQVAQAFGKWPV
                     VINEDGYKIKLFEWGVIADYMNTPEKIKEYRSSMANARSEKVLEDKRSVWHRIRHQRC
                     LVFTTGFFEHRDIKAKKKLPYFIKVKDEKLFCFAGFYNYSPLPDAETGEVIGTFAIIT
                     RPANAMLTKIHNAGPNSHRMPLVLTNELAKKWLDANLDDNEMKKILDFEFPDSELEAW
                     PVNTIRTRKEDNETVIAKIDTTLFPSL"
     gene            complement(2456..2719)
                     /locus_tag="PK977_08435"
     CDS             complement(2456..2719)
                     /locus_tag="PK977_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18181.1"
                     /translation="MAASQPEGFRIFINLMPAKWKASDSLKMKISQYVRFKLPWWNYS
                     PSDKLKVTLKERYGQLFISLLWRGQQTEVLLDDIEIFSVCAMT"
     gene            complement(2779..2967)
                     /locus_tag="PK977_08440"
     CDS             complement(2779..2967)
                     /locus_tag="PK977_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18182.1"
                     /translation="MNNPYSILTPEILNAMLKQPMYFVRQQYERGKDIFYNDQVPLLL
                     THYGHDETDRERAERHMR"
     gene            complement(2969..>3160)
                     /locus_tag="PK977_08445"
     CDS             complement(2969..>3160)
                     /locus_tag="PK977_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HRF18183.1"
                     /translation="SLFTRDVDHKKRYLMDAVDNINFSHRDDMVKYLASGLKRNWKMR
                     QEMRSKRYTTRWDELYEIM"
     assembly_gap    3161..3172
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3173..4254)
                     /locus_tag="PK977_08450"
     CDS             complement(<3173..4254)
                     /locus_tag="PK977_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRF18184.1"
                     /translation="MKAIVDCNSFYCSCERLFKPELKDKPVVVLSNNDGCIISRTDEA
                     KKLGVGMAAPYYQNKDIIEKNDVAVFSSNYHLYGDLSRRVMDTLRWLVGEENVEVYSV
                     DEAFLSLDTIPPDQLQAAAVRIKKTVEEWTGIAVSVGVAPTKVLSKVANRLSKKDKRG
                     TKGVMVLNDTSAIKQALEMTLVDDIWGVGRRYAMKLHQTGIDNGWQLSKMPEEWARKN
                     LGGVVGVRLIKELNSIACIPMKDPLETKKMIATTRMFGKPVFEVTEIKEAVATYTARA
                     AEKLRRQFSAATMLQVFVVTNGSRNEKYEYSPQSRYQYTTFPKATADTGELIRYALPL
                     AEKLYQPGLKYLKAGVILSGLVPDSS"
     gene            complement(4251..4685)
                     /gene="umuD"
                     /locus_tag="PK977_08455"
     CDS             complement(4251..4685)
                     /gene="umuD"
                     /locus_tag="PK977_08455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HRF18185.1"
                     /translation="MDGEDLFFGAAYKGSKQFTQQEVKTANATGFGAAADDYAERGID
                     LNEQLIRNKPATFFFRMKGDAMKNAGIFDGDILIVDRSLKLVNGKIIVAILNGELLVR
                     RFHKNFSFAFLIAENERYKNINLAEFSNFQVWGMVTYVIHTV"
     gene            4843..5475
                     /locus_tag="PK977_08460"
     CDS             4843..5475
                     /locus_tag="PK977_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRF18186.1"
                     /translation="METIINSIPGYRVYEYLLVLSPHEELRSRIIKVREEFAEKYKTD
                     NAGRSNPYITLVNFSQYAMMEERIISRLHTITMGFPPFKVELKDYGSFPSHTIYINVT
                     SKLPVQNLVKRIRTESQRLMKLNDDNKPHFIMEPHMTIGRKLKPWQYEKGWLEYSHKH
                     FTGRFIANEMTLLKRLAIESTDGYRMKYQVAATFQFQNLPVVTKQGELFG"
     gene            5616..6722
                     /locus_tag="PK977_08465"
     CDS             5616..6722
                     /locus_tag="PK977_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRF18187.1"
                     /translation="MPEKLKQDHFKVAVKKQEGLTYLTGRGAQFNTSNRFLKNETTKE
                     HIEGIDDWEESNVPTLYIEQESKTIVNKVESPDVGMSYSMNPYAGCEHGCIYCYARNV
                     HEYWGYSAGLDFERKIVVKKNAPELLRKFLMNKKWDATPIMLSGNTDCYQPAEQKYRL
                     TRGLLEVCNEFNQPVGILTKNSWILKDKDVLQEMAKKRIVSAMVSITSLNEDLRRVME
                     PRTTTAQQKLKVINELSSAGVRMGIMMGPMIPGLNEHEIQRIMKEARDNGATFTAYTF
                     IRLNGAIKLLFHDWLYKNFPDRADKVWHMVEQSHNGKVNDSRWGVRMRGEGSIAQLVA
                     QQYKKYGKLYGMNAEEWSLNRKLFRRPGEQGKLF"
     gene            complement(6714..>7089)
                     /locus_tag="PK977_08470"
     CDS             complement(6714..>7089)
                     /locus_tag="PK977_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRF18188.1"
                     /translation="LSAFIMKKDNGQQQSLYETKWSLKKIHTKQGTEEVNTKAFIKFN
                     QQKQSAGGNGSCNTFGSSITISGDKISFKDIFSTKMYCEGVQQVEDKFFQQLNKINRF
                     EIKDNTLLMLRDNEVLLEFQSE"
     assembly_gap    7090..7117
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7362..>9653
                     /locus_tag="PK977_08475"
     CDS             7362..>9653
                     /locus_tag="PK977_08475"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRF18189.1"
                     /translation="MRTFFLLLSGLIAFLPGFSQNDGRLLDWPMKLNYLSVKVNADAF
                     TATTFIEMEFCNPNDKEIEGLYLFQLEPGQAITAFQLELNGKYRDGSIEEKWKARNAY
                     NTIVGKRVDPALVQMDYANHYRLNIYPIPAKGCRRVTMTIQQQLKPVNGKLVYQLPLN
                     FKDTVKQIQITLRTANIKALPAIDKGLLKGQSFTINNNTYDLNWTGTNKRVDIPLSFS
                     LPLLLDKPALCIKQVKEKTFFALRFIPRINRAYAFNADNVLVFWDISASGRLRDIRKE
                     INFLKQYVSKHAVSRMTIITFNQQVQDTAIFFLNNNYQRWVGYLEALQYEGAVQYGSL
                     DFSAVKADAILLFSHGRNSFGADMPVPGQVHTWCINSATDKNQNHLENIIGFTGGQYI
                     DLANRNIEDAVALTTSTENILLRVKAGETTIDLNEKISVIKNDTVLLTGTLPAGTSSL
                     TLQYGNNSKAREEEIIEIATQGHCDISAIDRLEMLNRFEKYYGNNYYWYDVLNFGKAE
                     KVVTSSTSYIVLERIEDYIKFNILPPADLQPECDMSIFVKADEERRRQFNISNEFDVL
                     KSVVSAYNERIGRADKHGHSIILTDEKTNQIAGANNNQHLNTPAVANGLTGRVAGLAI
                     TSGGENAVAMSEVVVTAMGVTRQSKEVGYSVTRIHASELTQAKPVNLQQGLTGKVSGL
                     NVQTVNNGVFGDTRITLRGIRSLTGNNQPMLILDGSPVSLSLLNAINPNDVNNVTVLK
                     SAAATAIYGPDGVNGAVVVSTKKG"
     assembly_gap    9654..9729
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9750..>10156
                     /locus_tag="PK977_08480"
     CDS             9750..>10156
                     /locus_tag="PK977_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18190.1"
                     /translation="MKNLAARYEELREEYGREAAFYFDAAQVFYKAGLQKDAINILYS
                     ASAVTNGNVQVQEAIAYFLESWQQYDEAIKVYNCLLNTNPKELLYYRGLALTHYQRKD
                     YTNAVKQYYAGITLNTGEYEFYYKEYKAMLLQE"
BASE COUNT         2820 a         2032 c         2150 g         2981 t
ORIGIN      
        1 atgccagccg ggatggttct ccatcgcaag tagtagctac attattggtt actaaagcat
       61 aacgaagttt ttttgagtaa tgattcgatt gcaaaaaagc atcgattcca aatagcactt
      121 tagtcattcc ggtgtttcta atttagttct cccgcttttt catggtcatc gtatttatca
      181 cggagtttca gcaactcgtt tttgatttgt tgccggatct tttgatgagc aggcgaatta
      241 acgaggttct tttgttcttg cggatcattt ttcaggtcgt agagttccca ttcatttacc
      301 gtatagaaat aaatcagctt ataacggttg cctcttactg ctaaatgcgg aattacagta
      361 tggtctctta cgtactcata gtaatggtag tagagataat tcctgttcaa tgttttttgt
      421 ttacctgtaa ccaacggctt caggcttaca ccctgcatcc agccgggaat tttttcgcca
      481 gctatatcca gtattgtagg ggcataatct atgttttgaa ccattgtatt gatggtagtg
      541 cctgtcttga tctgaccagg ccatttcatc agcaacggaa cctgcataga aacatcatag
      601 gcgaaacgtt tatcaaacca gccattttct ccaagaaaaa agccctgatc actggtgtac
      661 atcactgctg tattcgatgc cagcttttct ttatccaggt agtccagaat gcgccctaca
      721 ctttcatcta tagaggcaac acaggccagg aagtcctgca tgtaccattg gtatttatat
      781 ttcagcagtt cattgccttt tggtcttaat tccctgagta attttcctct ttcagcataa
      841 atttctttgt accgttttct ttgttcctcc ggcacccggt tcatgatggc tttgtaataa
      901 gcgatttgtg ctgagtctgg cttatactca ggaatatcta ttagaaatgc aggatccact
      961 ttcaggtcgc tgcataattc catatcccgg agaatactca tggtttgcat gcgccatgca
     1021 cttccccggt taaatgtgtc gacataaagt gtagaaggtt cggggaatgt tttattgtga
     1081 tatgcttcca ggtgcttcag gttaggaaag aaatagcgat gaggggcttt atggtgaagt
     1141 aataaaagga aaggttttga tttatctctt ctttccagcc attttattgt ttcatctgta
     1201 attacatccg ttgcataacc ctgttgcgta atggtatcgc cgctcatttc aatcagtctc
     1261 gggtttacat agagaccctg tcccggcaat attttccagt aatcaaaacc tgttggatag
     1321 gagtgaaggt gccatttgcc aatgatagct gtttggtagc ctgcggcctg taacaacttt
     1381 ggcattgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnntccatt tttatgcgaa tgttgtcctg tgagcagggt ggccctggcc ggtccgcaaa
     1501 tggaattacc tacaaaagca ttggtaaatt tgacaccttc ctttaccagc cggtcaatat
     1561 tcggtgtctg aatcaacgtt ttattatagg cactgatggc atccgcatca tggtcatcgc
     1621 tcatgatgta aatgatgttg ggtctttgtt gtgccaccag gctacccgaa gccagaagca
     1681 ataaagacag aagcttttgc atgtcttatt ttttttaagt taatgcaatt ttattaaagc
     1741 gaggggaaca atgttgtatc aatttttgca ataacagttt cattatcttc ttttcttgtc
     1801 ctgattgtat ttacaggcca tgcttcaagt tctgaatcag gaaattcaaa gtcaagtatc
     1861 tttttcattt cattatcgtc cagatttgca tccagccatt tttttgccag ttcattagtt
     1921 aagactaatg gcatacgatg actgttgggt ccggcattgt gaatttttgt caacatcgca
     1981 ttagcaggcc tggtaataat ggcgaatgtg ccaattacct ctcctgtttc agcatcagga
     2041 agtggggaat aattatagaa acccgcaaag caaaatagtt tttcgtcttt tactttgata
     2101 aagtagggaa gtttcttttt agcctttata tcccggtgct caaaaaaacc agttgtaaat
     2161 accaggcatc tctggtgcct gatgcgatgc cagactgaac gtttatcttc cagcactttt
     2221 tcacttctgg cattagccat actgctgcgg tattccttta ttttctccgg agtgttcata
     2281 taatccgcta ttacacccca ttcaaataat ttgattttat atccgtcttc atttattact
     2341 accggccatt tgccaaatgc ctgtgccacc tggtgataag taggtgtaaa agaaatgcta
     2401 tctgatgctg ggatttgcag gtaatcactt atcagcctga tgttgctgaa aaaactcatg
     2461 tcatagcaca tacactgaag atttcgatgt catccaaaag tacttcagtt tgctggcctc
     2521 tccacaaaag ggagatgaat aattggccgt atcgttcttt aagggtcact ttcagtttat
     2581 cggaaggtga ataattccac caagggagct tgaatcttac atactggctg attttcattt
     2641 ttaaactatc actggctttc catttagccg gcatcaggtt gataaatatc ctgaaacctt
     2701 ctggttgcga tgctgccatg gttaactttt ctatatgttc cgggttgatg ctgtcatata
     2761 aaaacctgta ggggtctctt aacgcatatg tctctctgct ctttccctgt cggtttcatc
     2821 atgaccataa tgtgtaagta agagcggcac ctggtcgttg taaaaaatat ctttccctct
     2881 ttcgtactgc tggcgcacaa aatacatcgg ttgtttcagc attgcattca atatctcagg
     2941 tgtcaatatg gagtaggggt tattcatctc acattatttc atataattca tcccatcttg
     3001 tcgtatagcg tttgctgcgc atttcctgcc gcattttcca gtttcgtttc agaccggagg
     3061 caagatattt aaccatatca tcccggtggc tgaagttgat attatctact gcatccatca
     3121 ggtaccgttt tttatgatcc acatcccggg taaagagact nnnnnnnnnn nnattgagct
     3181 gtcaggcaca aggccactga gaataacgcc tgctttcagg tatttgagtc cgggctggta
     3241 aagcttttcg gccagcggca atgcataccg gatcagttca cctgtatcag cagtagcttt
     3301 tgggaaagta gtatactggt atcgtgactg cgggctgtat tcatactttt cattccggct
     3361 gccattcgtt accacaaata cctgcagcat cgtagcagca gaaaactgcc gtcgtagctt
     3421 ttcagctgct cttgctgtat atgttgccac agcttctttt atttcggtta cttcaaaaac
     3481 cggtttgcca aacattcttg tggtggcgat catttttttt gtttccagcg gatctttcat
     3541 aggtatacag gcgatgctgt ttagttcttt tatcagtctt accccgacga cgcctcccag
     3601 gttcttccgg gcccattctt cgggcatttt actcaattgc catccattat caattcctgt
     3661 ttggtgcagt ttcatagcgt atcggcggcc taccccccat atatcatcaa ccagtgtcat
     3721 ttccagcgcc tgctttattg cggaagtatc attcagtacc attacgcctt tggtgcctct
     3781 tttatctttt ttcgaaagac ggttggcaac ttttgataaa actttcgtag gtgctacacc
     3841 aaccgatacg gcaatgcctg tccattcttc tacggttttt ttaatacgga cggcggcagc
     3901 ttgtaattga tcaggtggta ttgtgtccag gctcagaaaa gcttcatcaa ctgagtatac
     3961 ttctacattt tcctctccca ccagccagcg taatgtatcc attactctcc tgctgagatc
     4021 gccatagagg tgataattac tcgaaaaaac agccacatca tttttttcaa ttatgtcttt
     4081 gttctgatag taaggcgccg ccattcctac gccgagtttt tttgcttcat cggttcggga
     4141 aatgatgcag ccatcgttgt tgctgagcac cacaacgggt ttatctttca gctccggttt
     4201 aaataaccgt tcgcaggaac aatagaaact gttacaatcg acaatggctt tcatactgta
     4261 tgaataacat acgtcaccat tccccatacc tggaaattgc tgaactctgc caggttgatg
     4321 tttttgtacc tttcgttttc agcaatcagg aaggcgaatg aaaagttttt atgaaaccgg
     4381 cgtaccagca gttccccgtt gagtatggct actattattt tcccgtttac cagtttgagt
     4441 gagcggtcta ctattaatat atcgccatca aagatgccgg cgtttttcat agcatcgcct
     4501 ttcatgcgga aaaaaaatgt agcaggtttg tttcgtatca gctgttcatt caggtcgatg
     4561 ccccgttcgg cataatcgtc tgctgcggcg ccaaagccgg tggcattggc tgtcttaacc
     4621 tcctgttgtg tgaattgttt tgagccttta taggcggccc caaaaaataa atcttctcca
     4681 tccatagcta gtcgttttac ccttgccggc aggtaccggt aaggaaatga tcaataatat
     4741 ttcaaaaatt taatggggtt attttttgta aagctaaata atttagtaaa ttagtactaa
     4801 gaaaatgggt aagtattttt tggtttttta aatggatgga tgatggaaac aattatcaac
     4861 agcatcccgg gttaccgggt gtatgagtat ttactggtac tcagtccgca tgaagagttg
     4921 cggagcagga tcataaaagt gagggaggaa tttgcggaaa agtataagac tgataatgca
     4981 gggaggagta atccctatat tactttggtt aatttttcac agtatgccat gatggaggaa
     5041 cgaattatca gccggctgca cactataact atgggttttc ctccattcaa agtagagtta
     5101 aaagattacg gcagttttcc ttctcatact atttatatca acgtaaccag caaactgccg
     5161 gtacagaacc tggtaaagcg tattcgaaca gaaagtcagc ggctgatgaa gctcaatgat
     5221 gataataagc cgcattttat catggagcca catatgacca ttggccgcaa actaaaaccc
     5281 tggcaatatg agaagggatg gcttgagtac agccacaaac attttaccgg ccggttcatt
     5341 gcgaatgaga tgacattgct aaaaagactg gcgattgaat caaccgatgg ctaccggatg
     5401 aagtaccagg tggcggcaac atttcagttt caaaacctgc cggtggtaac gaagcaggga
     5461 gaattgtttg gataataatc attccgttta cagtaacaaa acagtgtgat ggattagtgg
     5521 gtaaggagaa taaggagtaa gaaatgtgtg tatgattatg agagaaaact ctttattctc
     5581 cttttttatt tagaaaacgc aaaccaacca tgtgtatgcc tgaaaaatta aagcaagacc
     5641 actttaaggt ggcagtgaaa aaacaagagg gattaactta tttgaccggc aggggtgccc
     5701 agttcaatac cagcaatcgt ttcttaaaaa atgaaacgac caaagagcat atagagggga
     5761 ttgacgactg ggaagagagt aatgtgccaa ccctatatat agagcaggaa agcaagacca
     5821 ttgtcaataa agtggagagc ccggatgtag gtatgagtta cagtatgaat ccctatgccg
     5881 gatgcgaaca tggctgtatc tattgctatg cgagaaatgt gcatgaatac tggggctata
     5941 gcgccgggct ggattttgaa cggaaaattg ttgtaaaaaa gaatgcacca gaactactgc
     6001 gaaaattcct gatgaacaaa aagtgggatg ccacacctat catgctcagc ggtaataccg
     6061 attgctatca gcctgctgag caaaagtacc ggctgaccag gggtttgctt gaagtatgca
     6121 atgaattcaa ccagccggtt ggtatactta ccaaaaattc ctggatacta aaagataaag
     6181 atgtgctaca ggaaatggcg aagaaaagaa ttgtttcagc gatggtttcc atcacttcac
     6241 ttaacgaaga ccttcgaaga gtaatggaac caagaactac gacagcccaa caaaagctga
     6301 aagtaattaa tgagctcagc agcgcaggtg tgcggatggg catcatgatg ggaccgatga
     6361 tacccggact gaatgaacat gagattcaac gtatcatgaa agaagcaaga gataacggag
     6421 ctacttttac ggcttatacc tttatcaggc taaacggggc aatcaaactc ctgtttcatg
     6481 actggttata taagaatttt cccgacaggg cagataaagt atggcacatg gtggaacaaa
     6541 gtcataatgg taaagtgaac gatagtcgct ggggtgtgcg gatgcggggc gagggcagca
     6601 ttgcccagct agtggcgcag caatataaaa agtacggcaa gctttatggc atgaatgcag
     6661 aggagtggag cctgaaccga aaacttttca gaagacccgg agaacaagga aagttattct
     6721 gactgaaact ccagcagtac ttcattgtcc ctcagcatta ataatgtgtt gtcttttatt
     6781 tcaaaacggt ttattttatt tagttgctga aagaatttgt cttcaacctg ctgtactcct
     6841 tcgcaataca ttttagtgga gaaaatatcc ttaaagctga tcttatcacc gctgatggta
     6901 atgctgctgc caaatgtatt gcaactgcca ttgcctccgg cactttgctt ttgctggttg
     6961 aatttaataa atgcttttgt gtttacttct tctgtgccct gctttgtatg tatcttcttt
     7021 aaagaccatt tagtttcata aagagactgt tgctgaccat tgtccttctt catgataaat
     7081 gcggataaan nnnnnnnnnn nnnnnnnnnn nnnnnnngta ctcatataag ttatcagttt
     7141 catgtggtta ttttttaggt cgccccatcg gggcttattt atttgtcacc tgttatgcct
     7201 tcttaaacat tccctggtga tttgcagctt ttgttcatag gcatttcatt gtattatgtt
     7261 ttgaacagca taatgatgcc gccagtttgg ttcttacacg gctgaaaaat aatttatgca
     7321 attctttaac atttcgcatc ctgctggtaa atcactgcta tatgcgtaca tttttcctac
     7381 tgctgtcagg gcttattgcc ttcctgcctg gtttttcaca aaatgatggc cgcctgctcg
     7441 attggccgat gaaactgaat tacctgtctg ttaaagtgaa tgcagatgca tttacggcca
     7501 ccacttttat agaaatggaa ttttgtaacc ccaacgataa agaaatagaa gggctttacc
     7561 tgtttcaatt ggagccgggg caggccatta cggctttcca gttagaactg aacggcaaat
     7621 accgtgacgg gtccattgaa gagaaatgga aagcaagaaa tgcctataat accattgttg
     7681 gaaaacgggt agacccggca ctggtgcaaa tggattatgc caatcattac aggcttaata
     7741 tttatcctat accagcgaaa ggatgtcgaa gggtaacgat gacaattcaa caacagttaa
     7801 aaccagttaa cggcaaattg gtgtatcagc ttccactgaa tttcaaggat actgtaaagc
     7861 agattcaaat aaccctgaga acagcaaaca tcaaagcatt gccagctatt gacaagggtt
     7921 tactcaaagg acaaagtttt actattaaca ataatactta cgatttgaac tggactggta
     7981 ctaacaaacg ggttgatata ccgctttcat tttcactacc ccttttactt gacaagccag
     8041 ccttgtgtat aaaacaggta aaggaaaaaa cattctttgc ccttcgcttc ataccccgta
     8101 tcaacagggc ttatgcattc aatgcagata atgtattagt gttttgggat atttccgcca
     8161 gcggacggtt gagggacata cgaaaggaaa tcaattttct gaaacagtat gtatcaaaac
     8221 atgctgtatc ccgtatgact atcattacat tcaaccaaca ggtgcaagat acagccattt
     8281 ttttcctgaa caacaattat cagcgatggg tgggctacct ggaagcgcta caatatgaag
     8341 gcgccgtgca atacggcagt cttgatttct ctgctgtaaa agccgatgcc atactcctgt
     8401 tcagccacgg aaggaattct tttggcgcag atatgcctgt gccgggacag gtgcatacct
     8461 ggtgtatcaa ctcagcaact gacaaaaatc agaaccatct tgaaaatatt atcggcttta
     8521 ctggtggtca gtatattgat cttgccaaca ggaatattga agatgctgta gctttaacta
     8581 cttctacaga aaatatattg ctgagggtta aagcaggtga aacgactatt gaccttaatg
     8641 aaaaaataag tgtcatcaaa aatgatacag tactactgac gggtaccctg cctgccggaa
     8701 cttcatctct tacgctgcaa tatggaaata acagcaaagc aagggaagag gaaataatag
     8761 aaatagcaac tcaaggccat tgtgacatat cggccattga caggctggaa atgcttaacc
     8821 gttttgagaa gtattacggt aataattatt attggtacga cgtattaaat tttggcaaag
     8881 cagaaaaagt ggtcacttcc agcacttctt acattgtatt agagcgaata gaagattata
     8941 tcaagttcaa tatactgcca cccgcagatc ttcagccgga atgtgacatg agcatttttg
     9001 tgaaagctga tgaagaaaga agaagacaat ttaacatatc aaatgaattt gatgtgctga
     9061 aatctgttgt atcagcctat aatgaaagaa ttggccgggc tgataaacac ggacattcga
     9121 taatacttac tgatgaaaaa acaaaccaga tagcgggagc gaacaataac caacatttaa
     9181 ataccccggc ggttgccaac ggcttaacag gaagggttgc agggctggcg ataacaagtg
     9241 gaggagaaaa tgccgtagcc atgagcgaag tagtagtaac tgcgatggga gtaacccgtc
     9301 aatccaaaga agtaggctat tccgttacga gaattcatgc cagtgagtta acacaagcca
     9361 aaccggtaaa cctgcaacag ggattaaccg ggaaggtttc cgggctaaat gtgcaaaccg
     9421 tcaacaatgg tgtattcggc gatacccgaa taacgctgcg gggaatacga tcactcaccg
     9481 gtaataatca gcccatgctg atccttgatg gatcaccagt ttcgctgagt ttattaaatg
     9541 ccattaatcc caatgatgtg aacaatgtaa ccgttttgaa atctgctgcg gccactgcca
     9601 tttacgggcc agatggtgtt aacggtgccg tggtggtaag taccaaaaaa ggcnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnna ggaacttaaa caggcttcgg tgaaaaatct cgctgcccgt tatgaggagc
     9781 tgcgggaaga atatggccgg gaagctgcct tttattttga tgcggcacag gtgttctata
     9841 aagcaggact gcaaaaggat gcaatcaata tactttattc tgcttctgct gttactaatg
     9901 gcaatgtgca ggtgcaggaa gcgatcgcct acttcctgga atcatggcaa cagtatgatg
     9961 aagccatcaa ggtatataat tgtttattga acactaatcc caaagaactg ctttattaca
    10021 ggggccttgc tcttactcat tatcaaagga aggattatac caatgcggtg aagcaatatt
    10081 atgccggtat taccctgaat acaggtgaat atgagttcta ttacaaagaa tataaagcca
    10141 tgttgttgca ggaaat
//
LOCUS       DAORLS010000091        10058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3587_length_10058_cov_2.100570, whole genome shotgun sequence.
ACCESSION   DAORLS010000091 DAORLS010000000
VERSION     DAORLS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3587_length_10058_cov_2.100570"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>243
                     /locus_tag="PK977_08485"
     CDS             <1..>243
                     /locus_tag="PK977_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18191.1"
                     /translation="VPKDKIISSTTELSACEEAFGFPLVIKGKFYDAIIAYTMEQAQK
                     AFYKIAAKWGLPIIVQEFITGTEINIAVLGDEKGNAV"
     assembly_gap    244..367
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <368..637
                     /locus_tag="PK977_08490"
     CDS             <368..637
                     /locus_tag="PK977_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF18192.1"
                     /translation="NWKGGCELEIMRTSKDELYIMEINPRFPAWIYLTAAAGQNQPAA
                     LARMALGESQPVYTEYEVGKIFVRYSWDHITDIKEFHQVSGMGEL"
     gene            648..>1030
                     /locus_tag="PK977_08495"
     CDS             648..>1030
                     /locus_tag="PK977_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18193.1"
                     /translation="MEKLVYESPSIQKMNAGLMNKFGTRTDYEPVKQIDGVSVHSMMA
                     QYGSPLFVLSERTMRNNYQNAVRAFTTRYPKVHFAWSYKTNYLNAVCRIFHQEGSWAE
                     VVSGFEYRKALGNGVPGNKIIFNGP"
     assembly_gap    1031..1183
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1184..2022
                     /locus_tag="PK977_08500"
     CDS             <1184..2022
                     /locus_tag="PK977_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18194.1"
                     /translation="DTGVYPMWDRFGFNFENGQAWSALNKIVASGKMDLTGLHCHIGT
                     FMLSANAYATAAKKLGDLAWKCKTQLNTAIQYIDLGGGFPSTNTLRGSFLPGTDAVPS
                     VDEFAEAITKELLLLGFPPAELPLLVLESGRVLVDDAGYLLSTVLANKRLSDGRRATI
                     LDAGVNVLFTSFWYDHKITPAQDFTQHTEDMALYGPLCMSIDVVRQSVNLPLLNRGDR
                     VVIHKVGAYNMTQWMQFIQMRPNVILIDEQGQTHLIREAEKLEYVEQLEKVPHHLQQI
                     NI"
     gene            2047..>3094
                     /locus_tag="PK977_08505"
     CDS             2047..>3094
                     /locus_tag="PK977_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HRF18195.1"
                     /translation="MKKATIISFFDYITTTILNSYAMLFFSNKKFFAIIVLLSSFINP
                     YTGLAGLIATLIAALSAYFIGFSREQVKTGLFTYSGLLAGLGMGTFYEFSLGFWILLL
                     LASVLSVMLSAAFIAKLGRSGLPALSLAFIFTLWVVILSSREFAAIGLTQRNIYWLNE
                     MYATGGPDLIRFVQWLEDLQLPDAVRGFLLSLSAILFQSNIAAGILLTIGLLFFSRIA
                     ITLMVSGYAIAILFIQLMDGYAGSVNYYNLGTNFMLVSVALGGFYIIPSARSFIWSLI
                     TVPISYILVIALWKITYTWGLPVFSLPFCITVILFLFVLQLRKAGGKMVLTPVQYYSP
                     EENLYRYINSRERVM"
     assembly_gap    3095..3254
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3255..>4059
                     /locus_tag="PK977_08510"
     CDS             <3255..>4059
                     /locus_tag="PK977_08510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF18196.1"
                     /translation="YQGSGSTPEEYYCYNKPVLSPADGVVAEIIDHIEDNPIGGNNVQ
                     QNWGNTIVIKHAEGLYSKLSHLKKDSFKVAKGSFVKKGKIVAACGNSGRSPEPHLHFQ
                     VQATPYFDSKTMEYPLAYFIELKEGIAVLKSFAVPEQGTIAGNVVVNEQLQQAFSFQP
                     GMIMKVFSEGYKDEEWEVMVSAYNETYFYCRRHNDYAYFINNGTMFYFTHFFGRRRSL
                     LFLFYQAAFNVLMSTDKRITINDTFPRNTEGRHPLRWIQDLISPFYLFM"
     assembly_gap    4060..4129
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4248..5426
                     /locus_tag="PK977_08515"
     CDS             4248..5426
                     /locus_tag="PK977_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18197.1"
                     /translation="MRKLIITISLVFLMAILNVNAQHDFSFAAVDSQSYALYQKADWR
                     GLLAYGKLAIANKQDFLLLRLRTGYAAFMVENYSEAIKHYEAVLQEDKCNNTAHYFIY
                     WSRVNLGQPELAMAEAKFIAKDAIDEKKLKPSAVTGVETEFSIKRTSTRLRKNPFYAR
                     IGLANRLSHGFHMQQSVATYQQLINEPSLTAVSHNEKININQFEYYNRLMLNLDRHWQ
                     VKGAYHYLNTPFNNYTYNNHLLLLGVKYNGNYFDLQADAVIGKLTDTAIKQYNLQLGL
                     YPSGNLNLYSFSTVMLRQQGSTAFNFRQVIGTKLVKEVWLEGNITLGEFRNLVENDAL
                     YVYHAIDPNTMKAGVTSYILFGGKTTLQIGYTYEKRRLFNTTNEFQQHSITGGLSWKF
                     "
     gene            5438..6037
                     /locus_tag="PK977_08520"
     CDS             5438..6037
                     /locus_tag="PK977_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18198.1"
                     /translation="MAALLLATSLYRLNAQNEATLQAAFKESYTMESIRKFPEAIAAL
                     TKVYEEKSYELNLRIGWLYYLNKDYQQSQVYYQRAVNLKPYAIEAKLGLSQPLAALQY
                     TDKLLQLYEDVLKIDALHSVANYWAGVIHYNRLKYEDASKHFEKVVNLYPFDYDGNHM
                     LAWTYLNLGRSNEAKILFNKALLAKPGDTSCLDGLSKIK"
     assembly_gap    6042..6135
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6136..6469)
                     /locus_tag="PK977_08525"
     CDS             complement(<6136..6469)
                     /locus_tag="PK977_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18199.1"
                     /translation="MPTKIFVNLPVKDLNRSIKFFTHLGFSFNPQFTDEKAACLVINE
                     GSIYAMLLTEPFFAGFTKKQIADATKTTEVLIAIDVATKDQVTEIVNKAVEAGGSIYS
                     TPADHGWLY"
     gene            complement(6484..6780)
                     /locus_tag="PK977_08530"
     CDS             complement(6484..6780)
                     /locus_tag="PK977_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase alpha subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRF18200.1"
                     /translation="MRTGTKRKCEAGHIYYKSSDCPVCPVCEKKKSAVADFYSKLSAP
                     ARRALEGKGIFLIEQLARFTEKEILQLHGIGPASLPILRKALADEGLSFRNQKP"
     gene            complement(6783..7634)
                     /locus_tag="PK977_08535"
     CDS             complement(6783..7634)
                     /locus_tag="PK977_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF18201.1"
                     /translation="MIKLTFSATIYAPKQRVWYVLWEKSYYEQWTSLFAEGSTVKTDG
                     WKEGTKVLFVDPNNSGMLSVVAANKPYEFMSFRHIGMVNNGVEDTESEAVKSWAGATE
                     NYTLREESGFITLTVEMEITEEYKDYFNTTWPKALDKIKELSEVKTMITVSSTVNSPI
                     EKVWDSWTNPVHIVNWNNASDDWHTPMATNDLRKDGRFSFTMAAKDGSFSFDFGGTYT
                     SVEPEQKISYIMDDGRIAEILFEQDGSNIKITEIFQAENIHSLEMQEAGWQAILNNFK
                     KYTESLS"
     gene            complement(7631..8050)
                     /locus_tag="PK977_08540"
     CDS             complement(7631..8050)
                     /locus_tag="PK977_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF18202.1"
                     /translation="MKKITFTSVDEYIAGFQPAVRKLLTAMRKTIIAAAPNAEELISY
                     QMPAYKYKGVLVYFAAFEKHIGFYATPTGNSAFKKELSKYKTGKGSVQFPFTEPIPLD
                     LVKKIVLFRVKENETKALKKTAPARNKKSGLILKTKK"
     gene            complement(8047..8478)
                     /locus_tag="PK977_08545"
     CDS             complement(8047..8478)
                     /locus_tag="PK977_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF18203.1"
                     /translation="MKTASLHVYLTFNGNCREAMEFYRNCLGGQLTLQTVGDSPLCDQ
                     LPAQMKESILHASLVKDQLVLMGTDMVGEEGLKRGNTVSLMLDCCSEEEIRTCYDKLA
                     DGGVTTHPLHTSFWGALFGNLVDRFGNQWLLHFGKTIKPVS"
     gene            complement(8475..8855)
                     /locus_tag="PK977_08550"
     CDS             complement(8475..8855)
                     /locus_tag="PK977_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF18204.1"
                     /translation="MKKNTVNILYWVTTLLFAGFMIWSSVPGVSPKGQTLQVLHDYLG
                     YPVYFISFISVAKLVGCAAILVPGFDKIKEWAYAGLFFDLVGAIFSVVYVSKKFDPGM
                     LFILLPVALGVLSYYLWNRKKAMA"
     gene            complement(8913..9758)
                     /locus_tag="PK977_08555"
     CDS             complement(8913..9758)
                     /locus_tag="PK977_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF18205.1"
                     /translation="MKNNIHPCIWYNGNAKAAAEFYCSLFPESKITVDTPLVVNFELF
                     GQKFMDLNGGPMFKPNPSVSFFLISESDEEIDEWWAKLSKGGLIMMPLNKYEWAERYG
                     FLQDQFGLSWQIMKTAYSNVNQHITPCFLFVGNNYGKAAAAVHFYTKIFPASSITGIK
                     LYQEEEGERLAGKVKHSQFILDDLVFMAMDGFGPHEFAFNEGLSLVVECKDQATIDYY
                     WHKLIEGGGEESMCGWLKDPFGISWQIIPANIDRLIMDTEKGQRAMQALLKMRKIDIA
                     AMENA"
     gene            complement(9845..>10058)
                     /locus_tag="PK977_08560"
     CDS             complement(9845..>10058)
                     /locus_tag="PK977_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18206.1"
                     /translation="PEQMGFTVSYGNNIHISLHPDTRTETKRLFDALSAGGKVTYDLQ
                     DAFWGAYFGSCTDKYGVYWMFNCAEK"
BASE COUNT         2668 a         1955 c         2029 g         2805 t
ORIGIN      
        1 gttccgaaag ataagattat cagcagtaca actgaattga gtgcctgtga agaagcattt
       61 ggttttccac tggtgataaa agggaaattc tatgatgcca tcattgcgta tacaatggaa
      121 caggcacaaa aagcttttta taaaatagct gcaaaatggg gtctgcccat cattgtacag
      181 gagtttatta caggtacaga aatcaatatt gctgtgctgg gagatgaaaa gggtaatgcg
      241 gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnaac tggaaaggcg gttgtgaact ggagatcatg cggacgtcaa aagatgagtt
      421 gtacattatg gagatcaatc cccgcttccc ggcctggatc tacctgactg cagcagcggg
      481 tcaaaaccag ccggcagcat tggcaagaat ggcccttgga gaatcacaac ctgtttatac
      541 agagtatgaa gttggaaaaa tatttgttcg ctattcctgg gatcacatta ctgatatcaa
      601 agagtttcac caggtaagcg gaatgggaga attataaaaa aagaagtatg gaaaaactgg
      661 tatatgaaag cccttcgatc caaaagatga atgcagggct gatgaacaaa ttcggcacca
      721 gaacagacta tgagcctgta aagcaaattg acggagtatc tgtacatagt atgatggcgc
      781 aatatggatc tccgttattt gtattgagtg aaagaacgat gcgcaacaat tatcaaaacg
      841 ccgtcagggc atttactaca cgatatccca aagtgcactt tgcctggagt tataagacca
      901 attacctgaa tgccgtttgc agaatatttc accaggaggg cagctgggcc gaagtggtga
      961 gtggttttga gtatagaaaa gcgttaggta atggtgtgcc cggcaataaa ataattttca
     1021 atggtccgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggacac aggagtttac
     1201 cctatgtggg atcgttttgg ttttaatttt gagaacggcc aggcgtggag tgcattaaat
     1261 aaaatagtgg cctccggaaa aatggatctt accggcttgc attgccatat cggaacattt
     1321 atgttatctg ctaatgcgta tgcaacggct gcaaaaaaat taggtgacct tgcctggaaa
     1381 tgcaagacac aactaaatac cgctattcaa tatattgacc ttggcggcgg tttcccatcc
     1441 accaacacat taagaggttc atttttgcca ggaacagatg cggtgccttc ggtagatgaa
     1501 tttgcagagg caattacaaa agaattattg ctgcttggtt ttcctcccgc tgaattaccc
     1561 ttgcttgttt tagaaagcgg ccgtgtactg gtagatgatg ccggatatct gttatcaaca
     1621 gtactggcca ataagcggtt gagcgatggt cgccgtgcca cgatactgga tgccggtgta
     1681 aatgtcttgt ttacttcctt ttggtatgat cataagataa cccccgccca ggattttacg
     1741 caacatacag aagacatggc gctttacgga ccactatgta tgagtataga tgtagtgaga
     1801 cagagtgtaa accttccatt attaaacagg ggtgaccggg tggtgataca taaggtgggt
     1861 gcttataata tgacgcagtg gatgcaattt atccagatgc ggcccaatgt gatattgata
     1921 gacgaacagg ggcaaacaca tcttataaga gaagcagaaa aactggagta tgtagaacag
     1981 ttggaaaagg ttccgcatca tcttcagcaa atcaatatat gataaaatag caactggttt
     2041 cttcaggtga aaaaggcaac gatcatatca ttctttgatt atatcacaac aacaatattg
     2101 aacagctatg ccatgttgtt ctttagcaat aaaaaatttt ttgcaataat tgtattgctg
     2161 agttctttca ttaatcctta caccggcctg gccggcctta tcgcaacact tattgcagcg
     2221 ttatccgctt atttcatcgg gtttagcagg gagcaggtaa aaacggggtt atttacctac
     2281 agtggattac tagcagggct tggcatgggt actttttatg agtttagttt gggcttttgg
     2341 attttgttat tactggcatc cgttctttct gtaatgctat ctgctgcatt cattgccaaa
     2401 ctaggcaggt ctggtcttcc ggctttatcg cttgctttta tatttacatt gtgggtggtc
     2461 atattatctt ctcgggaatt tgcagctatc gggcttacgc agcgaaatat ttactggttg
     2521 aatgaaatgt atgccaccgg cggcccggac ctgatccgct tcgtgcagtg gttggaagac
     2581 ttgcagttac ctgatgcggt aaggggcttc ctgttgtcac ttagtgccat ccttttccag
     2641 agcaatattg cagcaggaat actcttgact attggtttac tttttttctc ccggatcgca
     2701 ataacattaa tggtatccgg gtatgcaata gccattttgt tcattcagct gatggacggc
     2761 tatgccggca gtgtgaatta ttataacctg ggcaccaact tcatgctggt atcagtagca
     2821 ctgggcggat tttatattat tccctctgcc cgttccttca tttggtcatt aattaccgta
     2881 cccatctcgt atatattggt cattgcgtta tggaagatca cctacacatg gggactgccc
     2941 gttttttctt tacccttttg tattactgta attcttttcc tgtttgtact gcaactacgc
     3001 aaagccggcg gtaaaatggt tttgacgcct gtacaatatt acagtccgga agaaaacctg
     3061 tatcggtata tcaacagcag ggaaagggtg atgannnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnncctatc aaggcagtgg aagcaccccg gaagagtatt actgctacaa
     3301 taaaccggta ctaagccctg ccgatggtgt agtggctgaa atcatagacc atattgagga
     3361 taatcccata ggaggaaaca atgtgcagca gaactggggc aacaccattg taattaaaca
     3421 tgcagaaggc ctctacagca aactttctca tttaaaaaaa gattctttca aagttgctaa
     3481 aggatcattt gtaaaaaaag gaaagatcgt tgcagcatgt ggaaattccg gccgttcacc
     3541 agagcctcat cttcattttc aggtacaggc tactccctat tttgattcga agacgatgga
     3601 gtatccactg gcttacttta ttgagttaaa agaaggcatt gcagtactga aaagttttgc
     3661 ggtgccggaa caagggacta ttgcaggaaa tgttgttgta aacgaacagc tgcagcaagc
     3721 attttctttt cagccgggta tgataatgaa agtattttct gaaggatata aagacgaaga
     3781 gtgggaagtg atggtgagtg cttataacga aacatatttc tactgccgcc gacacaacga
     3841 ttatgcttac ttcattaaca acggaacgat gttttatttc acccattttt ttggccgacg
     3901 ccgttcatta ttgttcctct tttaccaggc ggcttttaat gtgctgatga gtacggataa
     3961 aagaattaca attaacgata cgtttccacg gaacacagaa ggaagacatc cgcttcgatg
     4021 gatacaggac cttatttcac ctttttacct ttttatgagn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gatgcagcaa
     4141 cagcttggca gagcaataca agagaaaatg aatacccgga ttgatatcag aaggggaaaa
     4201 ataaaaatgt tccgggcagt atttaataac aaacaaatgg aagtgaaatg cgaaagttaa
     4261 taataacaat cagcctggtc tttttgatgg ccatactgaa tgtaaatgca caacatgatt
     4321 tttcttttgc agcagttgac agccagtctt atgccctgta ccaaaaagcc gactggagag
     4381 gccttcttgc atatggaaaa ttggcgattg ccaataaaca agattttctg ctgctacggc
     4441 tccgtaccgg gtacgcagct tttatggttg aaaactacag cgaggcgata aagcattatg
     4501 aggccgtatt gcaggaggat aaatgcaata acacagcgca ttattttatt tattggagca
     4561 gggtcaattt ggggcagccc gaattggcaa tggcggaagc taagttcatt gcaaaggatg
     4621 ctattgatga aaagaaactg aaaccttcgg cggttaccgg agttgaaact gaattcagca
     4681 ttaaacggac ttcaacccgg ctgcgaaaaa atccatttta tgcaagaatt ggtttggcta
     4741 atcgcctcag tcatggcttt catatgcagc aatctgtggc tacctaccag caactgatta
     4801 atgaaccttc attgaccgca gtatcgcaca atgaaaagat aaatatcaac cagtttgaat
     4861 attataatcg gcttatgctg aacctcgaca ggcactggca ggtaaaaggg gcctatcatt
     4921 accttaatac accatttaat aattatactt ataataatca cctgttgctt ttaggtgtaa
     4981 agtataatgg caattatttt gatttgcagg cagatgctgt cattggaaaa ttaactgata
     5041 ctgcaataaa acagtataac ctgcaactgg gcctgtatcc ttcgggtaat ctcaatcttt
     5101 acagcttttc aacagttatg cttcgccagc agggaagtac tgctttcaac ttcaggcagg
     5161 taataggcac aaaactggtg aaggaagtct ggcttgaagg gaacattacg ctgggtgagt
     5221 ttcgaaacct tgttgaaaat gatgccctgt acgtttatca tgccattgat cccaatacaa
     5281 tgaaggcagg cgttaccagt tatattttgt ttggcgggaa aactactttg cagattggct
     5341 atacttatga aaagcgccgg ctatttaata caacaaacga atttcaacaa cattcaataa
     5401 caggaggatt atcatggaag ttctgaaaag atcaattttg gcagccctgc tgcttgctac
     5461 ttctttatac aggctgaacg cacagaacga agcaacatta caggctgcgt tcaaagaaag
     5521 ttataccatg gaaagcatcc ggaagtttcc tgaggcaatt gcagcactca caaaagtgta
     5581 tgaagaaaaa agttatgagc tcaatttacg gatcggctgg ctgtattacc tgaacaagga
     5641 ttaccagcaa tcacaggttt attaccaacg ggcagtgaac ttaaagccat atgctataga
     5701 agcaaagctt ggactgtctc agccccttgc tgcattgcaa tacactgata agcttttgca
     5761 gctgtatgaa gatgttttaa agatcgatgc gctgcattct gttgccaact attgggccgg
     5821 ggttattcac tataaccgcc tcaagtatga ggatgcgtct aagcattttg aaaaagtggt
     5881 caacctgtat ccgtttgatt atgatggtaa ccacatgctg gcatggacct atttgaacct
     5941 tggcagaagc aatgaggcaa agattttgtt caacaaagcc ctgctggcca aacctggtga
     6001 tacatcctgc ctggatggac ttagcaagat taaataagat tnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnggtac agccagccgt gatcagccgg agtgctgtag atgctgccgc
     6181 cggcttctac ggccttgttc acaatttctg ttacctgatc tttagtggct acgtctatcg
     6241 ctattaatac ctcggtggtt ttagtagcat cggcaatttg tttttttgta aaacctgcaa
     6301 aaaagggctc tgtcagcagc atggcgtata tacttccttc gttgataacg aggcaggctg
     6361 ctttttcgtc ggtgaactgt ggattaaaag aaaagccaag atgagtgaag aacttaattg
     6421 agcggtttaa atcctttacc ggaaggttta caaagatttt tgttggcata aaaatcttat
     6481 tttttatggt ttttgatttc taaaagagag accttcatca gccagtgctt ttcttagtat
     6541 gggcagagat gcagggccta tcccgtgcaa ttgaagaatt tctttttcag tgaaacgggc
     6601 aagctgctca attaagaaaa taccctttcc ttccagggca cggcgtgccg gtgcactcag
     6661 ctttgaataa aaatcggcca ctgcactttt ctttttttca caaaccgggc atacagggca
     6721 atcactgctt ttgtaataga tatgcccggc ctcacatttt cttttcgttc cggttcgcat
     6781 gatcaggaca gactttctgt atattttttg aaattattca gtatggcctg ccagcctgct
     6841 tcctgcattt ctaatgaatg aatgttttcg gcctggaaaa tttctgtgat cttgatatta
     6901 ctgccatctt gttcaaacaa aatctccgct attcttccat catccatgat atagctgatc
     6961 ttttgttccg gctcaactga ggtgtaagtg ccgccaaaat caaaactgaa gctgccatcc
     7021 tttgccgcca ttgtaaatga gaaccggccg tctttgcgca gatcgtttgt ggccatagga
     7081 gtatgccagt catcagaagc attgttccag tttacaatat gtacaggatt ggtccagctg
     7141 tcccaaactt tttcaatcgg cgagttgaca gtagaggaaa cagtaatcat tgtttttact
     7201 tcagacagtt cttttatttt atccaatgct ttgggccagg tggtgttgaa gtaatctttg
     7261 tactcttccg ttatttccat ttcaactgta agtgtgataa aaccactttc ttctcttaac
     7321 gtataatttt cagttgcccc ggcccaggat ttcacggctt cactttcggt atcttcgaca
     7381 ccattattta ccataccgat gtgccggaag gacataaatt cataaggctt attggcggcc
     7441 acaacactta acatgccgct attattcgga tcaacaaaca gcactttggt accttctttc
     7501 catccatcgg tttttacggt cgagccttct gcaaaaaggc tggtccattg ctcatagtat
     7561 gatttttccc acaacacata ccaaactctt tgtttgggtg catagattgt agcagaaaat
     7621 gtaagtttta tcattttttt gtttttaaaa tgagacctga ttttttattt cttgccgggg
     7681 ctgttttttt caaagctttt gtttcatttt cttttactct gaatagcaca atctttttta
     7741 caagatcaag cggaatgggt tcggtaaatg gaaattgtac tgagcctttt cctgttttat
     7801 acttagagag ctctttttta aatgcactat taccagttgg cgtggcatag aagccgatat
     7861 gtttttcaaa ggcagcaaaa tataccaata cacccttgta tttatacgcc ggcatctggt
     7921 agctgatcag ttcttcagca tttggtgctg cagcaataat tgtcttgcgc atagccgtta
     7981 atagttttct cacagccggt tgaaaaccgg caatatattc atcaacacta gtgaatgtta
     8041 tttttttcat gaaaccggtt tgatggtttt accaaaatgc agcagccatt ggttgccaaa
     8101 tctatccaca aggttgccga acagggctcc ccaaaaactg gtgtgcaacg ggtgtgtggt
     8161 tacgccgcca tcagccaatt tatcataaca ggtcctgatc tcttcttcgc tgcagcaatc
     8221 cagcatcagt gatacagtat ttcctctttt taatccttct tctcctacca tatcagtgcc
     8281 cattagaacc aactgatctt ttaccaggga tgcatgcagg atgctttctt tcatttgtgc
     8341 gggtagctga tcacaaagcg gcgaatcacc aacggtctgc agcgttaatt gtcccccaag
     8401 acaatttctg taaaactcca tggcttcccg gcaatttccg ttaaaggtca ggtatacatg
     8461 aagagatgcg gtcttcatgc cattgctttt ttcctgttcc ataaataata cgataaaact
     8521 cccagcgcca ctggcaacaa tatgaacaac atccccgggt caaatttctt tgacacatac
     8581 actactgaaa agatagcccc gaccaggtcg aagaacaagc cggcgtaggc ccactctttt
     8641 attttatcaa agcccggtac cagtatagcg gcacagccaa ccagcttggc tacactgatg
     8701 aatgaaataa agtaaacagg ataccctaag taatcatgca gcacctgcaa ggtttgccct
     8761 ttgggtgaaa ctcctggcac ggatgaccag atcataaaac ctgcaaacag caaggtggtt
     8821 acccagtaaa gaatgttgac agtatttttc ttcatgttaa atgccgtttt tttaaataat
     8881 cagtgccttt tgtgttttag tgggaaatac cattatgcat tttccattgc agctatatca
     8941 atttttctca ttttcagcaa tgcctgcata gcccgttgtc ctttttccgt atccattatt
     9001 agccggtcaa tatttgccgg aatgatttgc caggaaatac cgaaaggatc tttcagccag
     9061 ccacacatac tttcctctcc gcccccttcg atcagtttat gccagtaata atctatagtt
     9121 gcctggtctt tacattctac tactaaagaa agtccttcat tgaaagcaaa ttcatgcggg
     9181 ccaaaaccat ccatggccat gaatactaaa tcatccagga taaactggga gtgtttcact
     9241 tttcctgcca gccgttctcc ttcttcttcc tggtatagct tgatgccggt gatggaggaa
     9301 gccggaaaaa tttttgtgta aaaatgtacc gcagctgctg ctttaccata attgttgcct
     9361 acaaataaaa agcagggggt gatgtgttgg tttacattgc tgtatgctgt tttcatgatc
     9421 tgccaggaaa gtccaaattg gtcctgtaag aaaccatatc gttctgccca ttcatattta
     9481 ttcagtggca tcataataag accgccttta gaaagtttgg cccaccattc atcgatctct
     9541 tcgtctgatt cactgatcag aaaaaacgaa accgatggat taggcttaaa catcggcccg
     9601 ccatttaggt ccatgaattt ttgtccaaac agctcaaagt ttacaaccaa tggcgtatcc
     9661 accgtgatct ttgattcagg gaacagtgag caataaaatt cagcagctgc tttggcattg
     9721 ccattgtacc agatgcaggg gtgtatattg tttttcattg tagttgataa gaagatgaat
     9781 atttttttct actaatagca tcacccttct gcccttcgac aggctcaggg tgacagcaat
     9841 gcaattattt ctcagcacaa ttaaacatcc agtacactcc atatttatca gtacaactgc
     9901 cgaagtaagc accccagaaa gcatcctgca gatcataagt caccttgccg cctgctgaaa
     9961 gcgcatcaaa taatcgtttg gtttctgttc ttgtatccgg gtgcaggctg atatgaatat
    10021 tgtttccgta gctcactgta aatcccattt gttccggt
//
LOCUS       DAORLS010000092        10028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3601_length_10028_cov_2.411010, whole genome shotgun sequence.
ACCESSION   DAORLS010000092 DAORLS010000000
VERSION     DAORLS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3601_length_10028_cov_2.411010"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1813)
                     /locus_tag="PK977_08565"
     CDS             complement(77..1813)
                     /locus_tag="PK977_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18207.1"
                     /translation="MKQAFLKWLSLLLLPVTLHSQQFDGNYNGQFSGDLNGHFTFTID
                     HKDYNNLEGTFIIKGGPTKIIVGMVKPDGSIDAYLYDKKTDITSALWKGKFTGRINNG
                     QTNGNYEVYDASAAKPTTSKGTWNKTATAIKPQGPPLSYSYITGKDLPFNEDKPIALR
                     FHLKLNDSWQKEYSIQFITIGARNPLQGRFAPDVDITDGFYVYTTESGLEITPAPGAK
                     EVDFNFEYAHFGSKEKLLLPKTVNAIFYVTLHNPQTGETRKEKLEAAASITSLCAILV
                     NKCNLGDCPSLNGKKIAKGSSVNAVSGDQLRIPLLAELWVKFLDGTVGYFSNKSQTPW
                     TLTMRGGHFSSTQQWEYTENTISIQSIAEKGIEKAADEISDKAMEAGLQLLLKKSASV
                     SPGLIVQLMEFMGASKAGGDLVAIKLRSKVEVSIYRNGTYRIRNFEGSPEIWQKNSKP
                     LLLPVGQQVFIDKKGIAGKPVSITDKPLAPEKTVTTQWAGTWQTKWGKMTITQNGNSI
                     TGTYEHDKGKISGTINGNKLSGNWSEAATYQPPGDAGEFEFTLSADGKSFTGRWRYGN
                     NGAWQTGWDGHR"
     gene            2075..2296
                     /locus_tag="PK977_08570"
     CDS             2075..2296
                     /locus_tag="PK977_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18208.1"
                     /translation="MQNLPPKDLVVYPCTHTALAKAYGISRKMLYKKLKPHAAEIGPR
                     KGYKYDLEQLLFIFEMLGWPPNPIPEYGE"
     gene            complement(2299..2814)
                     /locus_tag="PK977_08575"
     CDS             complement(2299..2814)
                     /locus_tag="PK977_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18209.1"
                     /translation="MKIQLILFSLLLSIIANAQNLKTFSATMRPDSLCYLSIKSGKAY
                     TKDAAGEVKTTLDLGLFETKNDKSSIIEWYNLKPDNEKVPEALCGTKTKVAAIGFDRE
                     QFDKCKTAADLKRMTSYISTNSFSHFAVVRNSNDYYQRCFIIEREDGKRGLIFMTATG
                     GNAFKAEVKAE"
     gene            complement(2972..4816)
                     /locus_tag="PK977_08580"
     CDS             complement(2972..4816)
                     /locus_tag="PK977_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18210.1"
                     /translation="MKKLLLLFSLVPVLVKVNAQNVGIGTTTPAAKLDVYSTSGFTSK
                     FNSASNQMYVSFHENNSLRGYIGSYAGAAEDFDIGTGSGNPLGKLHFTIQAGPKMTID
                     NAGNVGIGTTSPSSSSLLDLNSTTKGMLLPRMTSAQRNDIVYPATGLLVFDTDTKTIW
                     AFDGDNWKNLYNTGGGLILPYSQTVNTAAHAFHITNQGTGAGLAGSSTNEFGIGINAS
                     ATGGFGWGLNAFTNRPGAVSVRSVADTGMAFYGENIYAGNTNTLMYLLNRGLEKTLRL
                     QLSNSSNTMPNMQISGNNLGEQLKIFQTNAANTTPAVSIDNAGSGEGLKSVAANSGTG
                     VLGISSTGYGVKGESNSSSGFGGVRGDNTGSAGSGVIGVSNAANTQGVYGTSTNGIGV
                     RAFSNTYRGLQGTSASGTGVYASSTSGLALETVGNIKISGGNTNPADGAILTSDANGN
                     AVWKKNKIAFKTEHVAVDYYVIPSESEWKLMFSTELYDYGNNFEPYTGGVPGANDATF
                     IAPVNGLYHFDIKIGFEGAIYPNEIEDTYIILRRQRGASITDLAKSHGSVIYNWSLID
                     HADYNISTDVQLQAGDKLWVILWHDSDLGGDGKLQVYETFSGHLIFEH"
     gene            complement(4841..5173)
                     /locus_tag="PK977_08585"
     CDS             complement(4841..5173)
                     /locus_tag="PK977_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF18211.1"
                     /translation="MTQQPTITLTTSEGRFFFTPEEIIRLEASSNYTKIFFRNKTKMV
                     AAKVLKEFVTVLEPLGFVRTHRTHLVNRQHILCVTTGGNIIMRDASVAEISRRMKSGV
                     MKVLKNAA"
     gene            5538..>5805
                     /locus_tag="PK977_08590"
     CDS             5538..>5805
                     /locus_tag="PK977_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF18212.1"
                     /translation="MTGKVLSQSPGFIFSHLNTKEGLSDNVVTCFLKDSQGRLWIGTI
                     NGLNRYDGAHFYVFKKSADNHSLFNGNITALCEDKAGNIWGSTEA"
     assembly_gap    5806..5829
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5830..8530
                     /locus_tag="PK977_08595"
     CDS             <5830..8530
                     /locus_tag="PK977_08595"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF18213.1"
                     /translation="NPLTGRFRKIATPNRSNDKSFTNVLCDQEGNIWATGLWTLLKYN
                     PQIDKFEEQLVLDEHPQQAPDIGTRSNGLLIDTAEKEIWITTRAGLVCYEMANGKKYD
                     FSNQPGKKLFDRQFISALARSPGGLFWCFNNSTREIILFDPAKKEIVRTIGVSHIIPG
                     AGGVSLYEDKNHRLWFSSWTGDCLVIDYLHGNKMEKLTNRVDDKMSIANGYFTSVLED
                     EDGILWFGTVSGISKCSPDKNLYKVHYLRDKIPDLKNTSISVISEDPADKTWWMATID
                     RKIINYSPATGNYRTYIPDKAVPDKKNQLPGGVFNVTPVGNLVVIATATGAWQINKTT
                     HTITPFAYYPAAFSDFTGKYATALGDSVCFLTDGKQILRINLQTGKFIHYAYPGLENE
                     MGSIGFMFTDNEGRIWANSNLGRIVYTTADNQLATVKLVKDEENEFSGYFNTLDPDNK
                     GNIWVVSTGFGLYRYHPSTGKIKYWDQTIGLLSPYIQKNKTDRDGNVWLLTYSNVSVF
                     LPATNDFYHFTIPFAENTINYFNSINRLENGNIIASILNEVFEFFPGRINLTPVKRRP
                     EISLVNASGKDIMITQQEKLLFQPQENTLRFRFGLLTDASQFPYQFEYRLEGAEEGWT
                     KSAGINEAIYNSLPAGDYKFRVRAIGMNNPWHTGEAVVSFTIKAPFYKTTWFFVLVAL
                     LVSGILFFLYRYRMAQKEKLMLLENKAQMLEKEKAMVMYESLKQQLNPHFLFNSLTSL
                     SGLIETNQQVAGDFLEQMSGIYRYILKNGNNETVSLKDEIEFVQLYINLQQTRFKKGL
                     QVNIRVPDEYLHYKIAPVTLQNLIENAIKHNVIDIGSPLVVDIFIEGDYLVVKNNLQR
                     KKVVETSNMKGLAQFTSLYRYLSELPVIIEETEREFIIKIPLI"
     gene            8543..9313
                     /locus_tag="PK977_08600"
     CDS             8543..9313
                     /locus_tag="PK977_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF18214.1"
                     /translation="MKAVIIEDEDIIAEVLENKIKKVAPDIEVIGRLTSLKTARRWLA
                     ENAEPDLFFMDIQLSDGVSFDIFNDFILKSPVIFTTAYDEYAIRAFKVNGVDYLLKPV
                     KETELAAAIDKCRKLADKKERSAADITALIQSLANPSAATKYKEKFIVNIRNQWMPVN
                     TKDIACFTKEVLNYIYLFNGEKYMVDYVTLDEVEELLNPGQFYRANRQFIINIDAIQT
                     VKPVENSKLIIRLKEPNHKLEIDMSRLKSPEFKKWMDR"
     gene            9417..9734
                     /locus_tag="PK977_08605"
     CDS             9417..9734
                     /locus_tag="PK977_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18215.1"
                     /translation="MNLLRRIQSIFLNKDKQKQAMEQLQWQSYLLQSGIPARAEVLEL
                     SVKDSVLHDYLVVYMWLKFKYQDRVIYQQVGTQINRKMLPKKNQLIHIKFLPENISQV
                     LVL"
BASE COUNT         2779 a         2055 c         2312 g         2858 t
ORIGIN      
        1 gggtaattgg ggcgtactcc ggtgatgcga tgaaaactat tatcccgttt tccgatagca
       61 gtattaataa aaaatattag cgatgaccgt cccagccggt ttgccaggcg ccgttgttgc
      121 cataacgcca gcgaccggta aaactttttc catcggctga aagagtgaat tcaaattcgc
      181 cggcatcacc gggtggctgg taagtggcgg cttcagacca gttgccgctg agcttgtttc
      241 cattgatggt gccgcttatt tttcccttat catgttcata agtgccggta atactattgc
      301 cgttttgagt gatggtcatt ttaccccatt tggtttgcca ggtgcctgcc cattgggtgg
      361 ttactgtttt ttcaggtgct aagggtttgt cggttatgga aacaggtttg ccggctattc
      421 cttttttatc gatgaaaacc tgctggccca ccggcagtaa taaaggcttg ctgttttttt
      481 gccagatctc cggcgagcct tcgaagttgc ggatgcggta agtaccatta cggtatatac
      541 ttacttccac ttttgaacga agtttgatgg ctaccaggtc gcccccggct ttggaggcac
      601 ccatgaattc catcaactgt acaataagcc ccggtgatac ggaagcactt tttttgagca
      661 gcaattgtaa gccggcttcc atggctttat cacttatttc atcggccgct ttttctattc
      721 ctttttcagc aatgctctga atgctgatgg tattttcggt gtattcccat tgctgggtgc
      781 tgctgaaatg cccgccccgc atggtcagcg tccacggtgt ttgcgattta ttggaaaagt
      841 agccaacggt gccatcgaga aactttaccc agagttcggc aagcaggggt atgcgcagct
      901 gatcgccgga tacggcattg accgaacttc cttttgcaat tttttttccg ttcagtgaag
      961 ggcagtcgcc taaattacat ttgttgacca gtatggcaca tagcgaagtg atgctggcgg
     1021 cggcttctag tttttctttc cgggtttcac cggtttgcgg gttgtgcagg gttacataaa
     1081 agatggcgtt tactgttttg ggcagcagta gtttttcttt actgccgaaa tgggcatatt
     1141 caaaattgaa atccacttct ttggcgccgg gtgcaggggt gatttcgagt ccgctttcgg
     1201 tagtatatac atagaagcca tccgtgatat ctacatcggg tgcaaaacgg ccctgcagtg
     1261 ggttgcgggc accgatggtg atgaactgga tggaatactc tttttgccag ctatcgttca
     1321 gcttcaggtg aaaacggagt gcaatgggtt tgtcttcatt gaagggaagg tctttaccag
     1381 tgatatagct ataggataaa ggagggccct gtggttttat agctgttgct gttttgttcc
     1441 atgttccttt ggaagtggtg ggctttgcgg cggatgcatc atacacttca tagttaccat
     1501 tggtctgccc gttattgatc ctgccggtaa attttccttt ccataatgca gaagtgatat
     1561 cagttttttt atcgtacaaa taagcatcaa tgctgccatc aggctttacc atgccgacaa
     1621 tgattttcgt gggcccgcct tttatgataa aggtgccttc caggttattg taatccttgt
     1681 ggtcgatggt gaatgtaaaa tgaccattaa ggtcgccgga gaattgtccg ttgtagttgc
     1741 cgtcaaattg ctgggagtgt aacgtaacgg gcagcaggag aagagaaagc catttcagga
     1801 atgcctgttt catagtcaat gctttggtaa ataaaagaac tataacaggc aagctaccta
     1861 ctgcggagaa aattatgaat gatgtatgtc agtgctggtt attcttcctg caggaacgta
     1921 acggactggc gtgcagccga aataatatcg gccctcgtta tccgtagata ctcggggcta
     1981 aagctatggc aactccctgt taacgccgta gtaaagccgt agataaagca tcagtctgtt
     2041 cccggtcggc taactttaca ggaccttttc agctatgcaa aacctgcctc ccaaagattt
     2101 agtagtgtac ccctgcaccc acacggcgct ggccaaagcc tatggcataa gccgaaaaat
     2161 gctgtataaa aagctaaagc cccatgcggc ggaaattggc ccgagaaagg gttataaata
     2221 tgacctggag cagttgctgt tcatattcga aatgctgggc tggccgccca accccattcc
     2281 ggaatatggt gaataatatt actcagcctt tacttctgct ttgaaggcat ttccacctgt
     2341 ggcagtcata aagatcagtc cccgtttgcc gtcttctctt tcaatgatga agcagcgctg
     2401 gtaataatcg ttgctgttgc gcaccacggc aaaatgcgaa aatgaattag tactgatata
     2461 gcttgtcatg cgtttgaggt cggcagcggt tttgcattta tcaaactgct cccggtcgaa
     2521 gccgatggcg gcaactttgg ttttggtacc gcagagtgct tcgggtactt tttcattatc
     2581 gggtttaagg ttgtaccatt cgataatgga ggacttatcg tttttggttt cgaagaggcc
     2641 gaggtcaagg gttgttttta cttcgcctgc ggcatcttta gtataagcct ttccgctttt
     2701 gatactgaga tagcagagac tgtcgggccg catagtggcg gaaaaggttt tgagattttg
     2761 ggcgttggca ataatgctga gcaacaggga aaacaggatg agctgaattt tcatatagct
     2821 ggttttgatg ctgaaagata aaagaatttg gataaggttg aacggtgagt ggttgaccag
     2881 ggttgccagg ccactccgga atccgctgtt ccggagtggc ggaatgtgaa ggcagtatta
     2941 ataatcctgt tctttaatta acaggcatcg tttaatgttc aaaaatgaga tgaccgctga
     3001 atgtttcata aacctgcagt tttccatcac cgcctaaatc cgaatcgtgc cataaaatga
     3061 cccaaagctt gtctcctgcc tgcaattgca catctgttga aatgttataa tcggcatggt
     3121 ctatcaatga ccagttataa ataacagagc catgtgattt tgcaaggtct gttatagagg
     3181 cgcccctctg tcttctgagg atgatgtatg tgtcttctat ttcattggga taaatagctc
     3241 cttcgaaccc gattttaata tcaaaatgat aaaggccgtt tacgggggca ataaaagtag
     3301 catcgttggc gccgggcaca cctcctgtat aaggttcaaa attgtttcca taatcataaa
     3361 gctcggtgct gaacatcagc ttccattctg attcggatgg tattacatag taatcgacag
     3421 caacatgctc cgttttaaat gcaattttat ttttcttcca gacagcgtta ccgtttgcat
     3481 cactggtcag tatagcgcca tccgccggat tggtattgcc tcctgatatc ttgatattac
     3541 ctactgtttc caatgcaagt ccgctggtac tgctggcata tacacccgta ccacttgcag
     3601 atgttccctg taaaccacgg tacgtattgc tgaaagccct taccccgatt ccgtttgttg
     3661 aggtgccata aacgccttgt gtattggcgg cattcgaaac accaataaca ccgctgcctg
     3721 cggatcctgt attatctccc cgcacacctc caaacccgga agaagaattg gattcacctt
     3781 ttacaccata gcctgtcgag ctgataccca gcaccccggt tccgctgttg gctgcaacag
     3841 atttcagtcc ctcaccggaa cctgcgttat caattgatac agcaggggtg gtattggcag
     3901 cattggtctg gaaaattttt aattgttctc ccaggttatt tccgctgatc tgcatatttg
     3961 gcattgtatt actgctgttg gataattgca ggcggagtgt tttttccagg cctctgttga
     4021 gtaaatacat taatgtattg gtattgcctg catatatatt ctccccatag aatgccatac
     4081 ctgtatcggc cacggagcga accgaaactg cacccggtct gttggtaaaa gcgttcaaac
     4141 cccaaccaaa tccaccggta gcactggcat tgatgccaat tccaaattcg ttggttgatg
     4201 agccggcaag cccggcgcct gttccctggt tggtaatatg gaatgcatgt gctgctgtat
     4261 ttactgtttg tgaataggga aggattaacc cgccgccggt attgtataaa tttttccagt
     4321 tatcaccgtc aaaagcccag atggttttgg tgtctgtatc aaataccagc aggcctgttg
     4381 cagggtaaac aatatcattg cgctgtgctg aggtcattct cggcagcagc atccctttgg
     4441 tggtactgtt caggtcaagt aaggaacttg aggaaggtga agtagtacca atacctacat
     4501 taccggcatt atcaattgtc attttggggc cggcctgtat ggtgaagtgc agtttgccca
     4561 aagggtttcc gctgccggta ccaatatcaa agtcctctgc tgcaccagca tagctgccga
     4621 tatatcctct cagggaatta ttttcgtgaa aactcacata catctggtta ctggcagaat
     4681 tgaactttga tgtaaaacct gaagttgagt atacatcaag ttttgctgcc ggtgtcgttg
     4741 ttccaatacc tacattttgt gcatttactt taacaagaac cggaaccagg gagaagagaa
     4801 gaagtaattt tttcataatt gtggtttatt gttggggtag ttaggctgca ttttttaaga
     4861 ctttcatgac tccgcttttc atgcggcggc ttatttcagc aacagaggca tctctcatga
     4921 tgatattgcc gcccgttgtc acacaaagaa tatgctgacg gttgaccagg tgggtacggt
     4981 gcgttctgac aaaacccaat ggctccagta cggttacaaa ttccttcagc actttggccg
     5041 ctaccatttt tgttttatta cggaaaaaaa tcttggtgta gttactgctg gcttccaggc
     5101 ggatgatttc ttccggagtg aaaaagaacc tgccctctga agtggtaagg gtgatggttg
     5161 gttgttgtgt catacacatt ttgttttgac agcgtaaaaa tgataatcct ttttacccgg
     5221 taaaaaaatt acaggctcat atgcataatt ttccggttta tatgcgcagc gtattaatta
     5281 aaatgtaaaa aggggatggg gtggccacag catcggtaac aacgggtaca gggatgcggc
     5341 tcagttttaa tttattattt atgcacttcg attgaagttt tatgcagttc aatacatagt
     5401 tgcgcagtag caggggttaa acttgcagag gccggctgtt ttttttagct tgccggtagt
     5461 gcaacaacaa aatcatttca caaaagccaa tcctgttaac ggtgtttaga ctgacaggta
     5521 tattattctt tctattattg accgggaagg ttttatccca gtcaccgggt ttcatctttt
     5581 ctcacctgaa tacaaaagaa gggctcagcg ataatgtggt aacctgtttc ctgaaagaca
     5641 gccaaggaag gttatggata ggcactatca acggattgaa ccgttatgac ggggctcatt
     5701 tttatgtgtt caaaaaatct gctgataacc attccctgtt caacggaaat attacagccc
     5761 tgtgcgaaga taaagcgggg aatatctggg gaagtactga agctgnnnnn nnnnnnnnnn
     5821 nnnnnnnnnt aatccattga ccggccgctt ccgaaaaata gcaacaccca atagaagtaa
     5881 tgataaatcc tttaccaatg ttctttgcga ccaggaagga aatatatggg ctaccggatt
     5941 gtggacactt ttaaaatata acccgcaaat agacaaattt gaggaacaat tagtgctgga
     6001 tgaacatccg caacaggcac ctgatattgg caccaggtcc aacggactgc tgattgatac
     6061 agctgagaaa gaaatatgga ttacaacaag ggctgggttg gtatgttatg aaatggccaa
     6121 cggaaaaaaa tatgatttca gcaaccagcc cgggaaaaaa ttatttgaca gacaatttat
     6181 atccgcactg gcaagatctc ccggcggatt attctggtgt tttaataaca gcacaaggga
     6241 aattatctta tttgacccgg cgaaaaagga gatagttcgt acaatcggtg taagccacat
     6301 tattcctggc gccggcggtg tcagtttata tgaagacaaa aaccaccgcc tctggttcag
     6361 cagctggacc ggcgactgcc ttgtaattga ttatttgcac ggcaataaga tggaaaagtt
     6421 aacgaaccgt gttgatgata aaatgtccat tgctaacggt tatttcacat cggtactgga
     6481 agacgaagac ggcatcctgt ggtttggcac cgtgagtgga atttcgaagt gtagcccgga
     6541 taagaatctt tacaaagtcc attacttgcg ggataaaatt cctgatctta aaaatacatc
     6601 tatttctgtg atatcagaag atcctgcaga taaaacctgg tggatggcca ccatagacag
     6661 gaagattatt aattactcac cggcaaccgg taactacagg acctatatcc cggataaagc
     6721 agtgccggat aaaaagaacc agcttcccgg cggggttttt aatgttactc ccgtaggaaa
     6781 cctggtggtg attgctaccg ctactggtgc ctggcaaata aataagacta ctcatactat
     6841 tacccccttt gcctattatc ctgcagcgtt cagcgatttt accggtaaat atgcgacagc
     6901 gctgggcgac tcggtttgtt ttcttacaga tggtaaacaa atactcagaa tcaatttgca
     6961 gaccgggaaa tttatccact atgcttatcc gggcttagaa aatgaaatgg gcagtattgg
     7021 ttttatgttt actgacaatg aaggaaggat atgggctaac tcaaatcttg gaagaatagt
     7081 atatacaacc gctgataatc agctggcaac tgttaaactg gtaaaagatg aagagaacga
     7141 attcagcggg tattttaata cccttgatcc cgataataaa ggaaacattt gggtggtgag
     7201 caccggtttc ggcttgtaca ggtatcatcc atcaacaggc aaaataaaat actgggatca
     7261 gacaatcggc ctgctgtcac cctatattca aaaaaataaa acagaccggg atggcaatgt
     7321 ctggttgctt acatacagca acgtttctgt ctttttacct gctacaaacg atttttatca
     7381 ttttaccata ccatttgccg agaatacgat taattacttc aactcaatta accggctgga
     7441 aaacggtaat atcatagctt ccattttaaa tgaggttttt gaattttttc ccggcagaat
     7501 aaatcttaca ccggtaaaaa gaagacccga gataagcctg gtaaacgcaa gcgggaagga
     7561 tatcatgatc acacaacagg aaaaactatt attccagccg caggaaaata cccttcgttt
     7621 caggtttgga ttactaaccg atgccagcca gtttccttat caatttgagt accgcctgga
     7681 aggagctgaa gagggctgga caaaatcagc cggtataaat gaagccattt ataatagcct
     7741 gccagccggc gattacaaat tcagggttag ggcaattgga atgaataatc catggcatac
     7801 cggggaagca gtagtgagct ttaccattaa agctcctttt tataaaacta cctggttctt
     7861 tgttttagtt gccttgctgg tttcaggaat tttatttttc ctgtaccgct accggatggc
     7921 acaaaaagaa aaactgatgc ttttggaaaa caaggcgcag atgctggaaa aagaaaaagc
     7981 aatggtgatg tatgaaagcc tcaagcagca gctcaacccg cacttcctct ttaactcgct
     8041 gacgtccttg tctggtctta ttgaaaccaa ccagcaggtg gccggtgatt ttcttgaaca
     8101 gatgtccggt atataccggt atatcctgaa gaatggtaat aatgaaacag tatcattaaa
     8161 agatgaaatt gaatttgtac aactgtatat caacctgcag caaacaaggt ttaaaaaagg
     8221 gctgcaggtt aacatccgtg taccggacga atacctgcat tataaaattg caccggtaac
     8281 cttgcaaaac ctgattgaga atgccattaa gcataatgta atagatattg gctcaccgct
     8341 ggtggtagat atattcattg agggagacta cctggtggta aaaaacaacc tgcaaagaaa
     8401 gaaggtggta gaaacatcca atatgaaggg gctggcacag tttacttctc tttaccggta
     8461 cctgagtgag ctgcctgtta tcatagaaga aacggaaaga gagtttataa taaagattcc
     8521 attgatataa acaaacaagc atatgaaagc ggtaattatc gaagatgaag acattattgc
     8581 ggaagtgctg gaaaataaga ttaagaaagt ggcgccggat atagaagtga ttggccggct
     8641 gaccagcctg aagacagcac ggcgatggct ggccgaaaat gcggagcccg atcttttttt
     8701 tatggatata cagctaagtg atggtgtgag ttttgacatt ttcaatgatt tcatcctgaa
     8761 aagccctgta atatttacaa cggcttatga tgaatatgcc atccgtgctt ttaaagtaaa
     8821 cggggtggat tatttattga agccggtaaa ggaaacggaa ctggctgctg ctatcgacaa
     8881 atgccggaaa ctggcggata aaaaagaaag atcagctgca gatataactg cgttaataca
     8941 atctcttgcc aacccttctg ctgcaacaaa gtacaaggag aaatttattg taaacatccg
     9001 caaccagtgg atgccggtga atacaaaaga tattgcctgc tttaccaaag aagtattaaa
     9061 ctatatctat cttttcaacg gggagaaata catggttgac tatgttaccc ttgatgaagt
     9121 ggaagagttg ctgaaccctg ggcagtttta ccgggctaac cgtcaattca tcataaatat
     9181 agatgctata cagacagtaa agccggttga aaattctaaa ctcattattc ggttaaaaga
     9241 accgaatcat aaactggaaa tagatatgag ccgcctgaag agcccggagt ttaagaagtg
     9301 gatggaccgc taagtacact tgaatgtata aagtatacgt tttatatatc ctaatctgca
     9361 gttgacctgt ataaatttca gcgccttgtt gatctggaag aattttgttg gttagaatga
     9421 atttactcag gcgcatacaa tctatattcc tcaataaaga caagcaaaag caggcaatgg
     9481 agcagctgca atggcaaagc tatcttttgc aatcaggaat acctgcccgg gcagaagtac
     9541 ttgagttgtc cgtaaaagat tcggtgctgc acgactacct ggttgtttat atgtggctga
     9601 aatttaaata ccaggacagg gtgatttatc aacaagtcgg cacacagatc aacaggaaga
     9661 tgcttccgaa aaaaaaccag ctcatccaca ttaagttcct gccggagaat atttcacagg
     9721 tgctggtatt atagtttgtt tttcagcttc gcagtatttc gggctcttgt ttacaggaaa
     9781 ctgcccggca agatgctatg ttttgtggca ttcaatcttt gatttcatta ctactgtcaa
     9841 taccggcagg aacacaaaaa tcctttgcag gtacatttta attaaaaaat caggcaggtg
     9901 gagctataaa actttttttt gggtgctgct cttcacagtt ttacaccgta atcaaaaatc
     9961 aaaaaacgaa atcaacaagt atgaaaaatt aatcttagca attatgtcag ctgcaatttt
    10021 tgcatcct
//
LOCUS       DAORLS010000093        10012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3619_length_10012_cov_1.826353, whole genome shotgun sequence.
ACCESSION   DAORLS010000093 DAORLS010000000
VERSION     DAORLS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3619_length_10012_cov_1.826353"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PK977_08610"
     CDS             complement(<1..162)
                     /locus_tag="PK977_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRF18216.1"
                     /translation="MILPIVAYGHPVLRKVAEDITPEYPQLDKFIEDMWETMYASNGV
                     GLAAPQVNRS"
     gene            complement(206..619)
                     /gene="ruvX"
                     /locus_tag="PK977_08615"
     CDS             complement(206..619)
                     /gene="ruvX"
                     /locus_tag="PK977_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRF18217.1"
                     /translation="MPRILCIDYGLKRTGIAVTDPLQIIATGLTTIHPKDLITFLKDY
                     FAREQVELILIGWPTNWDDSATHATAHVEKCIKELQKHFPQLPVKKVDERYTSKLAKD
                     AMLEMGLKKMQRRNKALVDEIAATIMLQEYLRVNG"
     gene            complement(635..1627)
                     /locus_tag="PK977_08620"
     CDS             complement(635..1627)
                     /locus_tag="PK977_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRF18218.1"
                     /translation="MSKVKSYLSLIKFSHTIFAMPFALIGFFVGLFTFINSRYNDGQW
                     NLNKAISLKWDFESDSLPFLENTLAGKLAAGDATILTVGKYLLLVILCMVFARSAAMA
                     FNRYLDRQYDAKNPRTAVREIPSGVISAKNALVFTIVSCVLFLVSCYFINRLCFYLSP
                     VALAVVLGYSYTKRFTPLCHLILGLGLSLAPIGAYLAVTGQFAFLPILFSLAVIFWVS
                     GFDIIYALQDEEFDKSQQLYSIPAWLGKAKALRVSEVLHLLSAAAVIYAGVYGHFGWL
                     YWIGTVVFAGMLVYQHSVVKPHDLKRVNLAFMTANGIASVVFAIFVIADLFLNY"
     gene            complement(1635..2180)
                     /locus_tag="PK977_08625"
     CDS             complement(1635..2180)
                     /locus_tag="PK977_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRF18219.1"
                     /translation="MRKLSMQELGRKSVEEFKASDKIPIIIVLENIRSAYNVGSVFRT
                     SDAFLIEAIYIIGYSAKPPHKEIKKTALGAEETVNWKYFKTTAEAIEDLRSLGYNVYA
                     AEQAEGSYKLNAIGFEPEEKIAVVFGNEVTGVEQSTIALCDGCLEIPQLGMKHSLNIA
                     TAAGVVLWELVRSRIMLNDKF"
     gene            complement(2283..>3410)
                     /locus_tag="PK977_08630"
     CDS             complement(2283..>3410)
                     /locus_tag="PK977_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRF18220.1"
                     /translation="KRINLINAVTPVKQKPYGQVFLQVMCKRAGEIPVELSYYTNNAG
                     WTAMYDVRVNSKTNKVKLVYKASLTQTTGIDWKKTKLTLSTGTPNFGVTAPVLTPWYL
                     QLYVPGLYSDLQRRAMQGNAARNIIQSYGDDEKLAEVVVTDAAGQYKVKTQKDQTIDP
                     STLDQYTTLNSGQLNINYEIDLPYDIASDGQLNSINIKEQQIDCVLKNYAVPRVDKEA
                     YLLAEVADWQNLDLLPGDANIIMDDTYLGKSSIDPNTTADTLNLSLGRDKRVAVKRSL
                     VKEQSSLKTSGKDAKQVFTYEILVKNNKVSDVNLLLKDQFPLSTIKEVEVKLEDDGAA
                     MVNTETGVLTWKIDLKPGESKKVRFSYSVKYPKDKKIVNLK"
     assembly_gap    3411..3459
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3460..3995)
                     /locus_tag="PK977_08635"
     CDS             complement(<3460..3995)
                     /locus_tag="PK977_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4140 domain-containing protein"
                     /protein_id="HRF18221.1"
                     /translation="MKKLFLLTGIVLSCAHSFAQNNPAEKPDTVRADATISNATVYFG
                     YGAEMTHASKVTVGTNTRIIVISQLSTSIDANSLQISVPEDVALMSQRYAVFYPTPPP
                     VVKLKEVERLEDSINLLQKDISRLDNLIVIDQEILTKTGLLIESTVNNSGNKTITSDE
                     LLKLLEYYNGKIEKSKTN"
     gene            4067..>5156
                     /locus_tag="PK977_08640"
     CDS             4067..>5156
                     /locus_tag="PK977_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF18222.1"
                     /translation="MAKTASSDTPMMQQHRAIKQKYPDAILLFRVGDFYETFGQDAVL
                     ASQILGITLTKRNNGAAASSELAGFPHHALDTYLHKLVKAGHRVAICDQLEDPKLAKG
                     IVKRGVTDMVTPGTTINDKLLEHHSNNFLAALHFADNDQYGLAFLDISTGEFLIAEGD
                     REYADKLLQSFKPAEVVFQRHQQKKFKEYFSSKVYTYTLDEWIFDAAYAEETLLKHFQ
                     THSLKGFGVEELKNGLTAAGAIIHYLRDTEHPNLQHIVSLQRIDRDDFLWMDRFTIRN
                     LELVYGNSEGNHTLLNVLDNTVSPMGARLLKRWIVFPLKDIQKINERLDTVEYLIRET
                     ELRNKIAQHIKQCGDIERLVSKIPMKKIN"
     assembly_gap    5157..5172
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5173..>6409
                     /gene="mutS"
                     /locus_tag="PK977_08645"
     CDS             <5173..>6409
                     /gene="mutS"
                     /locus_tag="PK977_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF18223.1"
                     /translation="LKQVEVIKQTCLGSANEYLKRLGDALNPCPYIADKIFKEIVENP
                     PALAAKGGMMNSGVNAELDELRKIAFSGKEYLAQLQLKEAERTGIASLKIGFNNVFGY
                     YLEVTNSQKNKVPAEWMRKQTLANAERYITPELKEYEEKITGAEEKILRIEAELYEAL
                     LNELHDYLAPVQTNGNLLAIMDCLCCFAQNAIHYQYKKPQLHTGDEWIVTEGRHPVIE
                     RNLPIGESYISNNVELNKTDQQIIILTGPNMSGKSALLRQTALITLMAHMGSFVPASE
                     AKISLTDKIFTRVGASDNLSGGESTFMVEMNETASIINNITSRSLVLLDEIGRGTSTY
                     DGISIAWSIAEHLHNSPHQPKTLFATHYHELNELEEKMPRVKNYHVTNKEVGNKIIFL
                     RKLARGGSTHSFGIHVAKMA"
     assembly_gap    6410..6466
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6467..6695
                     /locus_tag="PK977_08650"
     CDS             <6467..6695
                     /locus_tag="PK977_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18224.1"
                     /translation="EVRSQQSEVGSPNTGNVLKGISSPKSDGQRMQLSIFDAHSETFD
                     TIRKMLEGIDINRLTPVEALLKLQEIKNSLK"
     gene            6783..7268
                     /locus_tag="PK977_08655"
     CDS             6783..7268
                     /locus_tag="PK977_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HRF18225.1"
                     /translation="MKLLKLTALPSLLLALLFFVASCEPDAELKKTTDFQKRDIPMTG
                     AQVVPTSASPALGKLNVFYTRETRILTYDFTWSGLSGNPTVGIGVYGLAPEGYAVSPT
                     TPIQTISTTGTTTTGSRSGTLLCDGVVIKEQDLLNGMYYIMLRTAAYPAGEIRAQIKF
                     Q"
     gene            complement(7318..7662)
                     /locus_tag="PK977_08660"
     CDS             complement(7318..7662)
                     /locus_tag="PK977_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRF18226.1"
                     /translation="MGRGVFTAENIEAGTVVEVSPVIIMSSEERKLLDQTLLHDYIFE
                     WGDDKRRSCMALGNVPIYNHSYESNCEYEMDYEREEIIIRTVRPVKAGEELFINYNGD
                     WNDSKPVWFDAQ"
     gene            7881..8291
                     /locus_tag="PK977_08665"
     CDS             7881..8291
                     /locus_tag="PK977_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18227.1"
                     /translation="MSLELEQPTLQEQFEAIIQTEDKLQIREFLDDQNISDVALLVEE
                     NPEHESQIIANMSVHRAASVFKIIDLSTQKRIIHELPPNTTASLLNELPADDRTDFLE
                     ELPSNVVRELIKLLDPDEKSHFHYWVTRKTVSAD"
     gene            8291..9265
                     /gene="mgtE"
                     /locus_tag="PK977_08670"
     CDS             8291..9265
                     /gene="mgtE"
                     /locus_tag="PK977_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRF18228.1"
                     /translation="MTPDYVYVYPWNTIEEVFTTIRKFGKDSETINVIYIINEKGELL
                     DDIRIREFILNPPDKKVEELMDERFVALHPEDDQETASEVFKMNNRVALPVVSNNNQL
                     LGIVTIDDILWVAEEEFSEDMQKMGGTEALEEPYLDVPIFKLYKKRIIWLFILFVGET
                     LTIAAMANFQETLEQVLVLSTFIPLIISSGGNSGSQAATLIIQALALGEITVQDWWRI
                     ARREILSGLFMGVTLGIVGFFIIYGGHQFFGLFGEHFIRIGFAIGTAIIGVVLWGTMM
                     GSMLPLLLKKLGADPATSSTPFIATLVDVTGLLIYFGTAFLLLKGVLL"
     assembly_gap    9565..9615
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9616..>10012
                     /locus_tag="PK977_08675"
     CDS             <9616..>10012
                     /locus_tag="PK977_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HRF18229.1"
                     /translation="RWATHGEPSDRNAHPHQSASGKLAMIHNGIIENYNQLKQELINK
                     GYTFHSDTDTEVLLNFIEEIRNNNQCDLEEAVRVALKRVTGAYVILLLDEDKPDTIIA
                     ARKGSPLVIGVGKGEHFLGSDASPMLEYTK"
BASE COUNT         2880 a         2246 c         2041 g         2672 t
ORIGIN      
        1 agaccggttc acctggggtg ctgccagccc aactccatta ctggcataca tggtctccca
       61 catatcctca atgaatttat ccaactgggg atattcaggt gtaatatctt ctgccacttt
      121 tcgtaaaacc gggtgtccgt atgccactat tggtaaaatc attgtgcaaa tttaccccgg
      181 agcccctaat aaaccaatta actaatcaac catttaccct aagatactcc tgcaacatta
      241 tcgttgcggc tatttcatcc accagcgcct tattgcggcg ctgcattttt ttaaggccca
      301 tttcgagcat cgcatctttg gccaatttgg aagtataacg ttcatccact tttttaacag
      361 gcagctgggg gaaatgtttc tgcagttctt tgatacattt ctccacatgt gcagtggcat
      421 gggtggcaga gtcatcccaa ttggtaggcc agccaatcaa aataagttca acttgttccc
      481 ttgcaaaata atctttcaga aatgtgatca ggtctttcgg gtgtatggta gtgaggccag
      541 tcgcaatgat ctgcaaagga tccgtaacgg cgatgccggt gcgtttgaga ccataatcga
      601 tgcagaggat tcttggcaat ggaatatttt ggggttaata attaagaaaa aggtctgcaa
      661 taacaaagat ggcgaaaaca acggaagcaa tgccattggc tgtcataaag gccaggttca
      721 ctctcttcag gtcatgaggc tttacaacgg aatgctggta caccaacata ccagcaaaga
      781 caactgtacc gatccagtat aaccagccga aatgaccata aacaccggca taaataaccg
      841 ctgcagcact caacaaatgc aatacctctg aaacccgcag cgccttcgct tttccaagcc
      901 aggcaggaat ggaatacaac tgctgtgatt tatcaaactc ttcatcctgc aaagcataaa
      961 tgatatcaaa accactgacc cagaaaataa cagctaatga aaaaagaatg ggcaaaaaag
     1021 caaactggcc tgtaacagcc aggtaagcac caattggcgc caatgataaa ccaagaccaa
     1081 ggattaaatg acataacggg gtaaagcgtt tggtataact atatcccagt accactgcca
     1141 atgctaccgg tgacaggtaa aaacaaagtc tgttgatgaa ataacaagaa acaagaaaca
     1201 aaacacaaga aacaatggta aaaaccaaag cattctttgc tgatattact cctgacggta
     1261 tttcccttac tgccgttctg gggttttttg catcatattg cctgtcaagg taacgattga
     1321 aagccatagc agcactgcgg gcaaatacca tgcaaaggat gactagcaac aagtacttcc
     1381 ctactgttaa aatggttgca tctcctgccg caagtttacc agccaatgtg ttttccaaaa
     1441 atggcagact atctgattca aaatcccact tcaaactgat tgccttgttc aaattccatt
     1501 gaccgtcatt atatctgctg ttaataaaag tgaataatcc aacaaaaaag ccaatcaacg
     1561 caaagggcat tgcgaaaatg gtgtgagaga attttataag gcttaaataa cttttgactt
     1621 tactcattca acatttaaaa tttatcattc aacataatcc tgctgcgaac cagttcccac
     1681 aaaacaaccc ccgctgccgt tgcaatattc agcgaatgct tcatacccaa ttgcggaatt
     1741 tctaaacagc catcacatag cgcaatagtt gattgttcca cacccgtcac ttcattgccg
     1801 aatacaacag ctattttttc ttccggttca aatccaatgg catttaattt ataactccct
     1861 tcggcctgct ctgcagcata gacattatat cccaggcttc ttaaatcttc gatagcttcg
     1921 gcagtcgttt taaaatattt ccagttcact gtttcctcag cgccaagggc cgtcttctta
     1981 atttctttat gaggtggttt ggctgaatag ccaataatat agatcgcttc aatgaggaaa
     2041 gcatcagatg tgcggaatac actgcctaca ttataggcgc tgcggatatt ctccagcaca
     2101 atgatgatcg ggattttatc agaggcctta aactcttcta ctgattttcg ccccagttcc
     2161 tgcatgctca gttttcgcat ggcgcaaaag tagggattag ttaaaaagtt aacaggttaa
     2221 taaagggcaa tatatcacaa ccctttatca acctgtcacc ttataaatct atcaaacaac
     2281 ctctacttca aattcacaat ctttttatcc ttcggatact tcacactata actgaatcgt
     2341 acttttttac tttcaccggg cttcaggtca atcttccatg tcagcacacc cgtttctgtg
     2401 ttgaccattg cagctccatc atcttccagt tttacttcca cttctttgat cgtactcaac
     2461 ggaaactgat cttttagcaa caggtttaca tcagacactt tattattttt taccaatatt
     2521 tcgtaagtga agacctgctt cgcatcctta ccggaggttt taaggctgct ttgctcttta
     2581 accaatgaac gcttaacagc cactcttttg tccctgccca aagaaaggtt caatgtatca
     2641 gccgttgtat taggatcaat agatgatttg ccaagataag tatcatccat aataatattg
     2701 gcatcaccgg gcaacaagtc aaggttttgc cagtcggcta cttctgccag caggtaggct
     2761 tctttatcca ccctgggcac cgcatagttc ttcagcacac aatcaatctg ctgttctttt
     2821 atattgatac tattgagctg accgtcgctg gcaatatcgt aaggcaggtc aatttcataa
     2881 ttgatattaa gctgaccaga attcagcgtg gtgtattgat ccagtgtact gggatcaatg
     2941 gtctggtctt tttgtgtttt tactttatac tgtcctgcag catctgtcac cactacttca
     3001 gccagttttt catcatcccc atatgattga atgatgtttc ttgcggcatt gccctgcatg
     3061 gctcttcgtt gaagatctga atacaaaccg ggtacataaa gctgtaggta ccagggagtc
     3121 aataccggtg ccgtgacacc gaaattaggg gtgccggtac tgagtgtaag ttttgttttc
     3181 ttccagtcaa tgccggtagt ttgtgtcagt gaagccttgt acaccagctt cactttattg
     3241 gtttttgaat tcactcttac atcatacata gctgtccagc ccgcattatt agtataatag
     3301 gatagctcta ccggaatctc tcccgccctt ttgcacatca cctgcaggaa cacctgaccg
     3361 tatggctttt gtttaaccgg cgtaacggcg ttgatcaggt tgatcctttt nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tattggtctt cgatttttca
     3481 atttttccgt tgtaatattc cagcagctta agcaattcat cactggtgat ggttttattg
     3541 ccgctgttat taactgtact ttcgatcagc aaaccggtct tggtcagtat ttcctggtcg
     3601 atgacgatca ggttatccag ccggctgatg tctttttgta aaaggttgat gctgtcttcc
     3661 agtctttcca cttcctttaa cttaaccaca ggtggcggcg tgggataaaa aacagcatat
     3721 cgctgcgaca tgagtgccac atcttccggt acacttatct gcaggctgtt ggcatcaata
     3781 ctggtactaa gctggctgat cactatgatc cgggtatttg ttccgacagt aactttcgaa
     3841 gcatgggtca tttcagcgcc gtaaccaaaa taaacggtgg cattgctgat ggtagcatca
     3901 gcccgtacgg tatcaggttt ttctgcagga ttattttggg caaaagagtg ggcacaggat
     3961 aatactatcc cggtcagcag gaataatttc ttcatggcaa atatgttttt ctacctgatg
     4021 caaaaagaaa gagtgttaca taaacacttc cttatttttg tcgcccatgg cgaaaacagc
     4081 aagttccgat acacccatga tgcagcagca ccgggccatt aagcagaaat accccgatgc
     4141 cattttgctg ttccgtgtcg gtgatttcta tgaaactttc ggccaggatg cggtactggc
     4201 ttcgcaaatc ctgggcatca cacttacgaa aagaaataac ggggcagccg cctcttctga
     4261 actggccggg tttccgcacc atgcattgga tacttatttg cacaaactgg taaaggcggg
     4321 tcaccgggta gccatctgcg accaactgga agatcccaaa ctggccaagg gtattgttaa
     4381 aagaggggtt accgatatgg taacacccgg caccaccatt aatgataaac tgctcgaaca
     4441 ccacagcaat aattttttag cagcccttca ttttgcagat aatgatcaat acggactggc
     4501 cttcctcgat atttctaccg gtgaattttt gattgcggaa ggcgacaggg aatatgcgga
     4561 taagctttta caaagtttca aaccggctga agttgtattt cagcgtcacc agcaaaagaa
     4621 attcaaagaa tacttcagca gtaaagtata cacctataca ctggacgaat ggatatttga
     4681 tgcggcttat gcagaagaaa ccttgctgaa acattttcaa acccattcac taaagggatt
     4741 tggcgttgaa gaactaaaaa atggactgac agctgccggc gccatcatac attatttaag
     4801 agataccgaa catcccaacc tgcagcatat tgtttcgttg caacggatag acagggatga
     4861 ttttttgtgg atggaccgtt ttaccatccg aaacctggaa ctggtttacg gcaactcaga
     4921 aggcaatcat accttattga atgtactgga caataccgtg tcacccatgg gggcaaggct
     4981 attgaaacgc tggatcgttt tccctttaaa ggatattcag aagatcaatg aaagattgga
     5041 cactgttgaa tacctgatca gggaaacaga actgcgcaat aaaatagccc agcacatcaa
     5101 acagtgcggc gatatagaaa ggctggtatc caagataccc atgaaaaaaa tcaaccnnnn
     5161 nnnnnnnnnn nngttgaaac aggtggaggt aataaaacaa acctgccttg gttccgccaa
     5221 tgaatattta aaaagactgg gtgatgcact gaacccctgt ccctatatag ctgataagat
     5281 atttaaagag attgttgaga atccccctgc ccttgctgcc aaaggcggca tgatgaacag
     5341 cggcgtaaat gcagaactgg atgaattgcg aaaaattgct tttagcggga aagaatacct
     5401 ggcacagcta cagctaaaag aagcggaaag aacgggtatc gcttcgttga aaattggctt
     5461 caataatgta tttggttatt acctggaagt aaccaattca caaaaaaata aggttcctgc
     5521 cgagtggatg cgcaagcaaa cactggccaa tgccgaacgt tacatcacac ctgagctaaa
     5581 agaatatgaa gaaaagataa ccggcgccga agaaaaaatc ttacgcattg aagcagaact
     5641 gtatgaagca ttgctaaatg agttgcatga ttatttagcc cctgtacaaa caaatggcaa
     5701 cctgctggcc attatggatt gcctctgctg ttttgcacag aatgccatcc attaccagta
     5761 caaaaaacca caactgcata caggagatga atggatcgtt acagaaggcc gtcaccccgt
     5821 aattgaaaga aacctgccga taggtgaaag ttatatcagc aacaatgtag aactgaacaa
     5881 gaccgatcag cagatcatca tccttaccgg tccgaatatg agtggtaaat ctgctttgtt
     5941 acggcaaaca gccctcatta ccctgatggc acatatgggt agttttgtac cggcgtccga
     6001 agccaaaatt tccttaacag ataaaatctt caccagggtc ggtgcctccg ataatttatc
     6061 aggcggtgaa tcaaccttca tggtagaaat gaatgaaaca gccagcatca tcaataatat
     6121 cacttcccgc agcctggtgc tgctcgacga gatcggaagg ggcacttcta cctatgacgg
     6181 tatttccatt gcctggagta ttgccgaaca cctgcataac tcgccgcatc agccgaaaac
     6241 attatttgct acacactatc atgagttgaa tgaactggaa gaaaaaatgc cccgggttaa
     6301 aaactaccat gttaccaata aggaagtggg caacaaaatc atcttcctgc gcaagctggc
     6361 aaggggcggc agtacccata gcttcggtat acatgtagcc aaaatggccn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggaa gtcagaagtc
     6481 agcagtcgga agtaggaagc ccgaatacag gaaatgtttt aaaaggaata agtagcccga
     6541 aatccgatgg tcagcggatg cagctttcca tctttgatgc ccacagcgaa accttcgata
     6601 cgataaggaa aatgctggaa ggaattgaca tcaaccggct gacacccgta gaagccctgc
     6661 taaaactcca ggagatcaaa aacagcctga aataaacagt tttcccacag ggttttaggc
     6721 tggttttata aaatattgcc aattcaaaaa acttcttatt tttaccacaa atctgtttca
     6781 tcatgaaatt gctaaaacta actgctttac cttctttatt attggcttta ttgttcttcg
     6841 ttgcatcctg cgaacccgat gcagaactga aaaagacaac tgatttccag aaaagagata
     6901 taccaatgac cggcgcacag gtggttccta ccagcgcatc accggcttta ggaaaactca
     6961 atgtttttta tacaagggaa acaaggatcc tgacttacga ttttacctgg tcaggcctta
     7021 gcggaaatcc aacggtaggt attggtgttt acggactggc acctgaggga tatgcagttt
     7081 ctcctacaac tcccatccag accatcagca ctacaggtac aacaactacc ggttcaaggt
     7141 caggtacatt gctttgtgat ggtgtggtga tcaaagaaca ggatctgctg aatggaatgt
     7201 attacattat gcttcgcacc gccgcctatc ctgccggtga aatcagggca cagattaagt
     7261 ttcaataata gtttttaacc gaatagattc aatggccttc attttggagg ccatttttta
     7321 ttgggcatca aaccaaacag gtttgctgtc gttccaatcg ccgttataat tgataaataa
     7381 ttcttcgccg gcctttactg gtcttactgt tctgataatg atctcttctc tctcataatc
     7441 catttcatac tcgcagttgc tttcataaga atgattataa ataggtacat tacccaaggc
     7501 catacagctg cgtcttttat catctcccca ttcaaaaata taatcatgca gcagggtctg
     7561 atcaagcaac ttcctttctt cactgctcat gataattaca ggtgaaacct ccaccaccgt
     7621 tccggcctct atgttttcgg ctgtaaaaac accccggccc atgtgatccg tttgtgcgat
     7681 atagagaaaa ggtaaaacca ttttaagtaa gaagttatac gtacgaggta cgaagtaagg
     7741 aaatcatccc ttaaatcgaa actttacaaa cgaagaaaac cacgaacttc taagtcacga
     7801 ctcacggttc ccgactaccg gttcattact cttcacttct tccctcgtac tgcatacttc
     7861 gtatttcgta tttttgacgc atgtcactgg aactggaaca acccacctta caggaacaat
     7921 ttgaagccat cattcaaaca gaagataagc ttcagatcag ggaattcctg gatgaccaga
     7981 acatcagtga tgtagccctg ctggtagaag aaaatcctga acatgaaagc cagatcatcg
     8041 ctaatatgtc ggtgcaccgg gcagccagtg ttttcaagat cattgatctg tctacccaga
     8101 aaagaatcat tcatgaactg ccaccgaata ctaccgcctc cctgctgaat gaactgcctg
     8161 cggatgaccg taccgacttc ctggaagaac tccccagcaa tgtggtaagg gaactaataa
     8221 agctgctgga cccggatgaa aaatcacact ttcactattg ggttacccgg aaaacagtat
     8281 cggccgattg atgacgcctg attatgtgta tgtgtatccc tggaatacca tcgaagaagt
     8341 attcaccacc atccgcaaat ttggcaaaga cagtgaaacg atcaacgtta tctatatcat
     8401 caacgaaaaa ggagaactgc tggacgatat ccgcatccgg gaattcattc tgaatccgcc
     8461 ggataaaaaa gtggaggaac tgatggatga gcgattcgta gccctgcatc ccgaggatga
     8521 ccaggaaact gccagcgaag tctttaagat gaataaccgg gtggcgctgc ccgtggtaag
     8581 caacaataac cagttgctgg gaattgtaac catcgatgat attctctggg ttgccgaaga
     8641 agaattcagt gaagacatgc agaaaatggg aggtaccgaa gccctggaag aaccttacct
     8701 ggatgtcccc atttttaaac tgtataagaa aaggattatc tggcttttca ttttgtttgt
     8761 tggtgaaacg ctgaccattg ccgccatggc caatttccag gaaacactgg aacaggtttt
     8821 ggtactttct acttttattc cgctgatcat ctccagcggg ggtaacagtg gttcgcaggc
     8881 agccacactg atcatacagg cattggcact gggtgaaata accgtgcagg actggtggcg
     8941 tattgcccgc agggaaattt tatccggcct gttcatgggc gttactttgg gtattgttgg
     9001 cttctttatt atttatgggg gtcaccagtt cttcggcctg tttggtgaac attttatccg
     9061 tatcggtttt gccattggta ccgccatcat cggtgtggtg ctgtggggaa cgatgatggg
     9121 atccatgctt cctttattac tcaaaaaatt gggcgccgac cctgccactt cttctactcc
     9181 atttattgcc acactggtag atgtgaccgg cttgctcatt tatttcggca cagctttcct
     9241 gctattgaaa ggtgtcttgc tttaaacgct gggccaaaaa tctaagtcaa tcaacgagat
     9301 aaataaactt tgcggtttct tgcaggtttg tttttgtcct ataccgaaat caatattttt
     9361 gtccgcaatt caaaatccat caatcaaaac cagtagtcat ttatgtgtgg aatagttggt
     9421 tacaccggac cccgggaagc ttatcctatc attatcaaag gccttaaacg gttagaatac
     9481 cgcggctacg acagtaccgg cgttgcttta cagaacggag gtctaaaagt ttacaagaaa
     9541 aaaggaaagg ttgcagaact cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnccgct gggccactca tggagagccc agcgaccgca atgcccatcc
     9661 gcatcaatcc gccagtggta aactggccat gatccataac ggtattattg aaaattacaa
     9721 ccagcttaaa caggaactga tcaataaagg ttacactttt cacagtgata ctgatacaga
     9781 agtattactc aactttattg aagagatacg caacaataac caatgcgacc tggaagaagc
     9841 tgttcgtgtg gcgctgaaaa gagttaccgg tgcttatgtt attttattac ttgatgaaga
     9901 taaacccgac accatcattg ctgcccgcaa gggaagtccg ctggtgattg gtgtaggtaa
     9961 aggtgaacat ttcctcggct ctgatgcatc acccatgctc gaatacacca aa
//
LOCUS       DAORLS010000094         9965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3645_length_9965_cov_1.634511, whole genome shotgun sequence.
ACCESSION   DAORLS010000094 DAORLS010000000
VERSION     DAORLS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_9965_cov_1.634511"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..609
                     /gene="nusG"
                     /locus_tag="PK977_08680"
     CDS             28..609
                     /gene="nusG"
                     /locus_tag="PK977_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRF18230.1"
                     /translation="MDAATANTENVQQQDTKWYVLRVVSGKEKKVKEYLDKEVSRGGW
                     DQVKQVFLPVEKVYKVANGKKVMRERNYYPGYVMIEIANGKLTDDLRDIIKNTTNVIH
                     FLGKENPIALRKAEVNKMLGKMDEMAEVGGISMSEPFIVGETIKIIEGPFNDFNGVIE
                     EVNDEKKKLKVTVKIFGRSTPVELNYMQVEKIS"
     gene            834..1277
                     /gene="rplK"
                     /locus_tag="PK977_08685"
     CDS             834..1277
                     /gene="rplK"
                     /locus_tag="PK977_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRF18231.1"
                     /translation="MAKEISGFVKLQCKGGQANPAPPIGPALGSKGINIMEFCKQFNA
                     RTQDKPGKVLPVLITVYSDKSFDFIIKTPPAAVQLMEAAKIQKGSKESNRSKVGKVTW
                     DQVKAIAEDKMPDLNCFTVESAMKMVAGTARSMGLNVEGKAPWEN"
     gene            1304..>1609
                     /locus_tag="PK977_08690"
     CDS             1304..>1609
                     /locus_tag="PK977_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRF18232.1"
                     /translation="MAFISKKRKVADTKVDANKAYSLKEASALVKQITTCKFDASVDL
                     HIRLGVDPKKADQAIRGTVTLPHGTGKTKRVLVLCTPDKEAEAKGAGADYVGLDEFVT
                     "
     assembly_gap    1610..1655
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1656..2013
                     /locus_tag="PK977_08695"
     CDS             <1656..2013
                     /locus_tag="PK977_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRF18233.1"
                     /translation="IIATPSVMPKIGKLGKVLGPRNLMPNPKTGTVTNDVANAINDVK
                     GGKIAFKVDKVGIIHASIGRVSFTPEKIAENSQELLNAIIKAKPSSAKGTYLKGISMA
                     STMSPGIAIDTKAFVH"
     gene            2041..2565
                     /gene="rplJ"
                     /locus_tag="PK977_08700"
     CDS             2041..2565
                     /gene="rplJ"
                     /locus_tag="PK977_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRF18234.1"
                     /translation="MTKDQKNEVIELLKGKFSQYNNFYVADTESLTVEQVGKLRRACF
                     NKQVEMKVAKNTLIRKALESLDNEKYAGMYDSLHKVTALMFSENPKEPALIISSFRKE
                     SNGEKPVLKAAFINGDVYMGDNNLKALTQIKTKNELIGEVIGLLQSPAKRVLAALLHH
                     HEQKAGGAEAAAAE"
     gene            2693..>3029
                     /gene="rplL"
                     /locus_tag="PK977_08705"
     CDS             2693..>3029
                     /gene="rplL"
                     /locus_tag="PK977_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRF18235.1"
                     /translation="MADVKSLAESLVGLTVKEVQELADFLKSEYGIEPAAAAVVVAAG
                     DGGGAAAAEEKTSFNVVLKSGGASKLNVVKIVKDLTGLGLKEAKDLVDGAPKNVKEGV
                     SKAEAEDIAA"
     assembly_gap    3030..3189
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3190..3895)
                     /locus_tag="PK977_08710"
     CDS             complement(<3190..3895)
                     /locus_tag="PK977_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF18236.1"
                     /translation="MKRLEIRMDDNERNPGAADYDDNNSFQYDRRSGHNNAGLKGELF
                     YFDPNSLSVEGWKKLGFREKIINTIRNYLSKGGKFKKPEDLKKIYGLFPDEYERIAAY
                     IRIADNNIPTHQEQEFRPIANNNSSNIKTGYAVIDINIADTTALIALPGIGSKLAARI
                     VNFRDKLGGFYSINQVAETYGLPDSTFQKIRQYLKLETNALRKININSATVDELKAHP
                     YIRYSLANPIVAYRNEH"
     assembly_gap    3951..3999
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4332..>4704
                     /locus_tag="PK977_08715"
     CDS             4332..>4704
                     /locus_tag="PK977_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18237.1"
                     /translation="MSSTKVNSRVDFGKIKHLAETPDLLEVQIQSFKEFFQLETTPDK
                     RNIEGLFRVFKENFPITDTRNIFVLEFLDYFIDPPRYTIEECMERGLTYAVPLKAKLR
                     LSCNDEEHVDFQTIVQDVFLGN"
     assembly_gap    4705..4764
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4765..>5092
                     /locus_tag="PK977_08720"
     CDS             <4765..>5092
                     /locus_tag="PK977_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18238.1"
                     /translation="SQLHRSPGVFFGQSVHPNGTKIYSARVIPFKGAWMEFATDINNV
                     MYAYIDRKKKFPVTTLLRSIGYETDKDILELFGMADEVKTDKKTLGKYVGKRLAARVL
                     RTWVEDF"
     assembly_gap    5093..5191
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5192..>8000
                     /gene="rpoB"
                     /locus_tag="PK977_08725"
     CDS             <5192..>8000
                     /gene="rpoB"
                     /locus_tag="PK977_08725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRF18239.1"
                     /translation="TIAEMDVKSVFIQREDVGGDYAIIYNTLNKDTSNSELEAVQFIY
                     RQLRGADAPDNETARGIIDKLFFSDKRYDLGEVGRYKINRKLGIDQPLEKKTLTKEDI
                     ILIIKYLVKLTNGKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRD
                     NEVFTPVDLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRE
                     RAGFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINDMGFIETPYRKVENGKV
                     DMKKLSFLSAEEEDLAKIAQANSGLNDKGEFVEEKVVSRQTGDFPILDKSEVEYMDVA
                     PNQIVGLSASLIPFLEHDDANRALMGSNMQRQAVPLIRPDVPIVGTGLEGKAARDARI
                     QIHAEGNGVVEFVDANEIHVRYERDEEEKLVSFEDDLRVYRLTKFIKTNQETCINLNP
                     AVKKGQKVKKGDFLTEGYATKNGELALGKNLKVAFMPWKGYNFEDAIVISERVVKEDM
                     FTSVHISEYELEVRDTKLGEEELTPDIPNVSEEATKDLDENGIIRIGAAVKEGDILIG
                     KITPKGESDPTPEEKLLRAIFGDKAGDAKDASLKAPNGVEGVVIGKKLFQRAKKDKNA
                     KVREKAAMEKLERIHEKNENDLMEVLLEKLTTLLANHVSAGVSNNFGEVQIGKGAKFT
                     EKNLRGLDYANINPLGWTGDKKVDDQINTLLHNYNIKFNEELGRYKREKFNISIGDEL
                     PAGVLKLAKVYLAVKRKLKVGDKMAGRHGNKGIVAKIVRQEDMPFLEDGTPVDVVLNP
                     LGVPSRMNLGQIYETILGWTGEKLGLKFSTPIFDGASVDEIADYCKQANIPMYGHTYL
                     YDGETGEKFHQKATVGIIYVIKLHHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRF
                     GEMEVWALEAYGASNILQELLT"
     assembly_gap    8001..8099
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8258..>8977
                     /locus_tag="PK977_08730"
     CDS             8258..>8977
                     /locus_tag="PK977_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRF18240.1"
                     /translation="MAIRKENRPKAAFSQITIGLASPDTILERSYGEILKPETINYRT
                     YKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYLLGVSSKKLETIVYYERFVVIQPGIRVDKGQNVGDLLT
                     EEEYLDILDTLPKDNQYLPDDDPNKFIAKMGAEAVHDMLARIDLDQLSFDLRNAAATE
                     TSQQRKADALKRLSVVEAFRDA"
     assembly_gap    8978..9168
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9169..>9965
                     /locus_tag="PK977_08735"
     CDS             <9169..>9965
                     /locus_tag="PK977_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRF18241.1"
                     /translation="KRMLQEAFDSLFDNSRKSNAVKAEGGRALKSLSDVLKGKQGRFR
                     QNLLGKRVDYSGRSVIVVGPELKLHECGLPKDMAAELFKPFIIRKLIERGIVKTVKSA
                     RKLVDKKEAVVWDILENILKGHPVMLNRAPTLHRLSIQAFQPKLIEGKAIQLHPLVCT
                     AFNADFDGDQMAVHVPLSHAAILEAQLLMLASHNILNPQNGTPITLPSQDMVLGLYYI
                     TKGKRTTATETVRGEGKIFYSSEEVIIAYNERVVDLHAWIKVKANIR"
BASE COUNT         2856 a         1724 c         2206 g         2475 t
ORIGIN      
        1 tttatttaca aacaactttt ctaaacaatg gatgcagcta cagcaaatac agaaaatgta
       61 caacagcagg ataccaaatg gtatgtattg cgggttgtaa gtggcaagga gaaaaaagtt
      121 aaggaatacc ttgataaaga agtgagccgt ggcggctggg atcaggtaaa acaggttttt
      181 ttacccgttg agaaagttta caaagttgcc aatgggaaaa aggtgatgcg ggaaagaaat
      241 tattatcccg gctatgtaat gatcgaaatt gccaatggta agcttactga cgacctccgg
      301 gatatcatta aaaatacaac caacgtaatt cattttcttg gaaaggaaaa tcccattgcc
      361 cttcgtaagg ctgaagtgaa caagatgctg ggcaaaatgg atgagatggc agaagtgggt
      421 ggaattagca tgagtgagcc gtttattgtg ggtgaaacga taaagattat tgaaggacca
      481 tttaatgatt ttaatggtgt tatcgaagaa gtaaacgatg aaaaaaagaa gctgaaagtg
      541 accgtaaaaa tatttggccg ttctacgcct gtggagctta attatatgca ggtagaaaaa
      601 attagctaag taattgactt ataatggcta aaagctaccg gttgccccgg tagctttttt
      661 gttggccgaa atgtaaaaaa atgtgcgtta aagccattgc tcatctgagt tttactcata
      721 cttttgcacc cccaattagt tctttagaaa tatccgttta ggcggattat gaggaatttg
      781 ggagaccgag taatctaaat gaaaggtcgt tagaaccaat aaatcaaaag aaaatggcaa
      841 aagaaatctc tggctttgtt aagctacagt gcaagggtgg ccaggccaac cccgcacctc
      901 cgatcggtcc tgcgctgggt tccaagggta ttaatatcat ggaattctgt aagcaattca
      961 atgcaagaac acaggacaaa cccggaaaag ttctccccgt attgattact gtttattcag
     1021 acaagagttt tgattttatc atcaaaacgc ctcccgcagc cgttcaactg atggaagccg
     1081 ctaagattca gaagggttcc aaagagagca accgcagcaa agtaggtaag gtaacatggg
     1141 accaggtaaa ggcaattgca gaagacaaga tgcctgacct gaactgtttt actgtggaaa
     1201 gcgccatgaa aatggtagcc ggcacagccc gcagcatggg tcttaatgta gaaggtaaag
     1261 ctccatggga aaattaattt tttgaatcca aaaacgaatt aaaatggcat tcattagtaa
     1321 aaaaagaaaa gttgctgata cgaaggttga cgcaaataaa gcatattcac tgaaggaggc
     1381 atctgcactt gtaaagcaaa tcaccacctg caagtttgat gcatcagttg atctgcacat
     1441 ccgtttgggt gtggatccta aaaaggctga ccaggccatc cggggtacgg tgacacttcc
     1501 tcatggtacc ggtaaaacga aaagagtatt ggttctttgt actcctgata aagaggctga
     1561 agcaaaaggt gctggtgcag attatgtagg tcttgatgaa tttgtaactn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatca tcgctactcc atctgtaatg
     1681 cctaagattg gtaaattggg taaagtgttg ggacctcgta acctgatgcc gaatcctaaa
     1741 acgggtactg ttactaacga tgtagcaaat gctatcaatg atgtaaaagg cggtaagata
     1801 gctttcaaag ttgataaagt aggtatcatc catgcctcaa tcggccgtgt aagttttaca
     1861 cctgagaaaa ttgcagaaaa cagccaggag ctgcttaatg ccattatcaa ggctaaacca
     1921 tcttcagcaa aaggtacata cttgaaagga attagcatgg ctagcactat gagtcccggt
     1981 attgcaattg acactaaagc atttgttcac taatctataa agtgattaaa actaattatc
     2041 atgacaaaag atcaaaaaaa cgaagtgatt gagctgctga aaggcaagtt ctctcaatat
     2101 aataacttct atgttgccga tacagaatca ctgactgtag agcaggtggg caaattgcgc
     2161 cgtgcatgct ttaacaaaca ggttgaaatg aaagtggcaa agaataccct tatcaggaag
     2221 gcgcttgaat cattggataa tgagaaatat gcaggtatgt atgattcgct gcataaagta
     2281 acagccctga tgttttcaga aaatccgaaa gagccggcgc tgatcatcag ctctttccgt
     2341 aaagaaagca atggtgaaaa accggtgctg aaagctgcat tcatcaatgg tgatgtatac
     2401 atgggcgata acaaccttaa ggctcttact caaatcaaga cgaagaatga gcttatcggt
     2461 gaagttattg gtctgttgca gtctcctgcc aagagggtat tggctgcatt attgcaccac
     2521 cacgaacaga aagccggcgg tgctgaagct gcggcagcgg agtaattgaa ttatttattg
     2581 catttgcaat caaaataacc acccaggcct gagggtcaac aaaggcaaat ttttttaaac
     2641 cgccgtcgga gcaactaaaa caagcagtga agacggtaaa aatttatatc aaatggcaga
     2701 cgtaaaatca ttagcagaaa gcctggtagg cttaacagta aaagaagtac aagaactggc
     2761 tgatttcctg aaatctgagt atggcatcga acctgctgca gcagcagtag tagtagctgc
     2821 cggcgatggc ggtggcgcag ctgctgctga agaaaaaaca tctttcaacg tagtgttgaa
     2881 gagcggcggt gcttcaaaac tgaacgttgt aaagatcgtt aaggacctga ctggtcttgg
     2941 actgaaagaa gctaaagacc tggtagacgg agctcctaag aatgtaaaag aaggtgtttc
     3001 taaagctgaa gcagaagata tcgcagctan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnc atgttcatta cgataagcta caattgggtt agcaaggcta taacggatat
     3241 atggatgggc ttttaattca tccacagtcg ccgagttgat atttatcttt ctcaatgcat
     3301 tggtttccag ctttaggtat tgcctgattt tttggaaggt agaatctggc aaaccataag
     3361 tttcagctac ctgattaatc gagtagaagc cacctagttt atccctgaag tttacaatcc
     3421 ttgcagccaa tttacttcca attcccggaa gggctattag ggctgtagta tcagctatat
     3481 tgatatcgat tacagcatat ccagttttga tgttagaaga attgttgttt gcgattggcc
     3541 gaaattcctg ttcttggtgg gtggggatat tgttgtccgc aattcggata taggctgcta
     3601 ttctttcata ttcatcaggg aacaagccgt aaatcttttt caggtcttct ggctttttga
     3661 atttgcctcc tttgctcagg taattgcgga tagtatttat tattttctcc cggaagccta
     3721 gctttttcca gccttcaact gataaactgt taggatcaaa ataaaaaagt tcccctttta
     3781 agccagcatt attatggccg ctacgcctgt catattggaa gctgttattg tcatcgtaat
     3841 cagctgcgcc gggatttctt tcattgtcat ccattctgat ttccagcctt ttcatcgctg
     3901 caatccaggc agtatctggt tgttgaattt ggaccttttg ttttgtagta nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taatggctat tctgtccctg
     4021 cgggtgaaag tgagataatc cgcaacaaat ttcggccagc tcatgaatgc ctctttaaag
     4081 gcaaaatagt aaaggatgaa acaccggaaa ataaccactt agtggtattt tggccgggtt
     4141 attgtgagca aacttttctt ggctgagaat tataaatcct tacctttgcc atcctttatt
     4201 ttggccaaat atattttgac atcccaaatc atgtgtaagt agttgacttc catgggtttt
     4261 cattttttct caaaatgaga gcctgtgagt gatttgtatg tcctcaacgt tgtaaaaccg
     4321 gtattacgca tatgtcttca acaaaagtga actcaagagt ggatttcggt aaaatcaagc
     4381 acctggctga aacccccgat cttcttgaag tacagatcca gtcattcaaa gaatttttcc
     4441 agttagaaac tacaccggac aaacgtaaca tcgaaggttt gttccgtgtg tttaaagaaa
     4501 attttccgat cactgatacc cggaatattt tcgttttgga gtttctggat tactttattg
     4561 atcctccccg ctataccatt gaagagtgta tggaaagggg gcttacttac gctgttccgc
     4621 ttaaggccaa actccgtttg agctgtaatg acgaggaaca tgtggacttc cagacaattg
     4681 tacaagacgt atttctgggt aatannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnngtccca gttgcaccgt tcacctggtg tattctttgg
     4801 tcagtcagtt cacccgaacg gaacgaagat ctactctgca agggtaatac cttttaaagg
     4861 agcctggatg gagtttgcga cagatatcaa caatgtaatg tacgcttaca ttgaccgtaa
     4921 gaaaaaattc cctgtaacta ctttgttgcg ttctatcggc tatgaaacag ataaggatat
     4981 cctcgagctg tttggcatgg ctgatgaggt aaaaacagat aaaaagacac ttggaaaata
     5041 tgttggaaaa cgtcttgctg caagggtatt gagaacatgg gtagaggact ttnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntactattgc tgaaatggat gtgaagagtg
     5221 tgttcattca gcgggaagat gtgggcggcg attatgccat tatctataat accctgaata
     5281 aggatacttc caacagtgaa ctggaggccg tacaattcat ctatcgccag ttgcgtggtg
     5341 cagatgctcc agataatgaa actgcccgtg gtatcatcga caagctgttc tttagcgaca
     5401 agcgttacga tcttggcgaa gtaggtcgtt acaagatcaa tcgcaaactt ggtatagatc
     5461 agccgcttga aaaaaagaca ttaacaaaag aggatattat cctcatcatt aaatacctgg
     5521 ttaaacttac aaacggtaaa gctgagatcg atgatattga tcacctgagc aaccgtcgtg
     5581 tacgtaccgt aggagagcag ttgtatgctc aatttggtgt gggtcttgcc cgtatggccc
     5641 gaactatccg tgaaaggatg aacgtaaggg ataacgaggt gtttaccccg gttgatctga
     5701 tcaatgccag aacgctttct tccgttatca attcattctt cggaacttca cagttatcac
     5761 aattccttga ccagacaaac ccattgagtg agataacaca caagcgtcgt atctcagcac
     5821 tcggtcccgg cggtttaagc cgtgaaagag caggcttcga ggttcgtgac gtacactatt
     5881 ctcactatgg ccgtttgtgt accattgaaa caccggaagg accaaacatt ggtttgatct
     5941 ccacactgtg tgttcatgcc aagatcaatg atatgggctt tattgagact ccctatcgta
     6001 aagtggaaaa tggcaaggtg gatatgaaaa aattgtcatt cctgagtgca gaagaagagg
     6061 acctggcaaa aattgcccag gccaactcag ggttgaatga caaaggggag tttgttgaag
     6121 agaaagtagt aagccgccag accggcgact tcccaatact ggataaaagc gaagtggaat
     6181 acatggatgt ggcgcctaac cagatcgttg gtttgagtgc ctctttgatt cccttccttg
     6241 agcatgatga tgctaaccgt gccctgatgg gatctaacat gcaacgacag gctgtacccc
     6301 tgattcgtcc tgatgtgccg attgtaggta ccgggctaga aggaaaagct gcccgtgatg
     6361 caaggattca gatccatgcg gaaggtaatg gtgtggttga atttgtggat gcaaacgaaa
     6421 ttcatgttcg ttatgaaaga gatgaagaag agaaattggt aagctttgaa gacgatctga
     6481 gagtttatag acttaccaag ttcattaaaa cgaaccagga aacttgtatc aacctcaatc
     6541 ctgcggttaa aaaaggtcag aaagttaaga agggagactt ccttaccgaa ggatatgcta
     6601 ccaagaatgg tgaactggca cttggtaaaa acctgaaagt ggcgttcatg ccatggaagg
     6661 ggtacaactt cgaggatgcg attgtaatca gcgaaagagt agttaaagaa gatatgttta
     6721 cttcagttca tatttccgag tatgaactcg aagtaagaga tacaaaattg ggagaagaag
     6781 agcttactcc tgatattccc aacgtttctg aggaagccac caaagacctt gatgagaacg
     6841 gtatcataag gattggtgca gcagttaagg aaggggatat cctgattggt aagattacac
     6901 caaaaggaga aagcgaccct acaccagaag aaaaacttct tcgtgccatc ttcggtgata
     6961 aagccggtga tgcaaaagat gcttcattga aagctccgaa tggtgtagaa ggtgtagtaa
     7021 ttggtaagaa attattccag agggctaaga aagacaagaa tgcaaaagtg agagaaaagg
     7081 cggctatgga gaagctggaa agaattcatg agaagaatga aaatgatctc atggaagtgc
     7141 tgctggaaaa gcttactacc ctgctggcaa atcatgtttc tgcaggtgtt agcaataact
     7201 ttggagaagt tcagattggt aaaggcgcca agttcactga gaaaaatctt cgtggccttg
     7261 attatgcaaa tatcaatccg ctgggatgga ccggtgataa gaaagttgac gaccagatta
     7321 ataccttgct tcacaactat aacatcaagt ttaatgaaga gcttggccgt tataagagag
     7381 agaaattcaa tatttcaatc ggtgatgaat tacctgcagg tgtattgaaa ttggcaaaag
     7441 tttatctcgc agttaagcgt aagctgaaag tgggtgataa gatggccggt cgtcacggta
     7501 acaaaggtat tgtagctaag attgttcgcc aggaggatat gccattcctg gaagatggaa
     7561 cacccgttga cgttgtattg aacccgttag gtgtacccag tcgtatgaac cttggtcaga
     7621 tctacgaaac gattctcggc tggacaggtg aaaaattagg attgaagttc tctacaccca
     7681 tttttgatgg agcttctgtt gatgaaattg ccgactactg taaacaggct aacataccaa
     7741 tgtatggcca cacttacctg tatgatggtg aaacgggaga gaaattccac cagaaagcaa
     7801 ctgtcggtat catttatgtt atcaaactgc atcacatggt tgatgacaag atgcatgccc
     7861 gttcaatagg gccttacagc ttgatcacac agcagccgct gggtggtaag gcacagttcg
     7921 gtggacagcg ttttggtgag atggaggtat gggcacttga agcttatggt gcatctaaca
     7981 ttctccagga attgttgaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     8101 aatgtattgg tacatgaatt gagaggtctg ggccttgacc tgaaatttga ataaagctca
     8161 gcagcgaaca gaaagctgaa gagtgcgacg caacgaagat gagtattgtt gatgtcgccg
     8221 gtaacaaaaa acataaacga caaacgataa acgtaacatg gcaatcagaa aagaaaatcg
     8281 tcccaaagcg gctttttcac agatcaccat cggtcttgct tctcctgata caattttgga
     8341 aagaagctat ggtgaaattt taaagcctga aactatcaac tatcgtacgt acaagcctga
     8401 aagagatggt ttgttttgcg aaaggatttt tggaccggtt aaagattacg aatgtgcttg
     8461 cggtaaatat aagcgtatcc ggtacaaagg catagtttgt gaccgatgcg gtgtggaggt
     8521 aacggagaag aaagttcgcc gtgaaagaat gggccatatt aaactggtag tacctgttgt
     8581 tcatatatgg tattttaaat cgcttcctaa taaaattggt tacctgttgg gtgtcagctc
     8641 caaaaaactg gagacaattg tttattacga aagatttgta gtcattcagc cgggcattcg
     8701 tgtcgacaaa ggacaaaatg ttggcgacct gctgactgaa gaagagtatc tcgatatcct
     8761 ggatacattg ccaaaagata accagtactt gccggatgat gatccaaaca agttcatcgc
     8821 taaaatgggt gctgaagcgg tacatgatat gctggcaagg attgaccttg accaattatc
     8881 attcgacttg cgtaatgcag cagctactga aacatcacag cagcgtaaag ctgatgctct
     8941 taagcgtttg agtgtggtgg aagctttccg tgacgccnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aaaaggatgc
     9181 tgcaggaggc ctttgactcc ctgtttgaca acagccgtaa atcaaatgct gtaaaagcag
     9241 aaggtggaag ggcactaaag tctcttagtg atgtgttgaa aggtaagcaa ggacgtttcc
     9301 gtcagaacct gttaggtaag agggttgact attctggtcg ttctgttatc gtggtaggtc
     9361 ctgaattgaa actgcatgag tgtggcctgc caaaagatat ggctgctgaa ttattcaagc
     9421 catttatcat ccgtaaatta attgaaaggg gtatcgtaaa aacagtgaag tctgcccgta
     9481 aactggtaga taagaaggaa gctgtggttt gggatatcct tgagaatatt ctgaagggac
     9541 acccggtaat gttgaaccgt gccccaacgc ttcaccgttt gtcaatacag gctttccagc
     9601 caaaactgat agaaggaaaa gcgatacagt tgcacccgtt ggtgtgtacg gctttcaacg
     9661 ccgactttga cggtgaccag atggccgttc atgtgccatt aagccatgct gctattctgg
     9721 aagcacagtt gctgatgctg gcttcacata atatcctgaa cccgcagaat ggtacaccta
     9781 tcacccttcc ttcccaggac atggtgttgg gtctttacta tataacaaaa ggtaaaagga
     9841 ctactgcaac agaaacagta agaggggaag gaaaaatatt ttattcaagt gaagaggtta
     9901 ttatcgctta caatgaaagg gtagttgacc tgcatgcatg gataaaggtg aaagccaata
     9961 tccgc
//
LOCUS       DAORLS010000095         9916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3670_length_9916_cov_1.838049, whole genome shotgun sequence.
ACCESSION   DAORLS010000095 DAORLS010000000
VERSION     DAORLS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3670_length_9916_cov_1.838049"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>224)
                     /locus_tag="PK977_08740"
     CDS             complement(<1..>224)
                     /locus_tag="PK977_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18242.1"
                     /translation="AYKIRVTGTAPPDTSINTKALTVKTIPSVNFSIEGPGIGCISAG
                     IHKYYPSQQEAGVNYNWSVSGGGTFTTNQDT"
     assembly_gap    225..332
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<333..3172)
                     /locus_tag="PK977_08745"
     CDS             complement(<333..3172)
                     /locus_tag="PK977_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18243.1"
                     /translation="MTRLIYFLILNALVLSQPALSQRKTTQKVNMSELENKVIIPQWA
                     SAHTIYSRSNLNRNMNAPEASCDTIILTRQTQIDSFKILYPGCTSVQILEINGLGASP
                     AITQLDSLDEIESVSQKLNISYTAITSLSDFAALTSLGDTLELQYNFLQTSIGLNNLT
                     NLGTIIINKLPALNTLSGLSNGITNIGGVLIDSTALTSLTGLESVEKINGYLDIRFSP
                     ILSLSSLSNLDSINGYLRLERLPSLTSLSLNNLEYVWGFLFSGLDNLTSLSGLSNNLL
                     NTNLGTIWMINTGLTSLSGLDNVTGCVNFYFWLNPNLTTLNGFNQVSGDIPGGFSLWA
                     NDVLTDISALSNITSISNGDLAFHGNNQLTDLTGLGNIITIGRRLRVYENPNITSLAF
                     LNNSLDIQDNNTEGIQIESNPQLSVCSFDPVCNYLNNGGFGIVQNNAPGCSSITEIQA
                     GCGPGCTSGIVKTWTGINGTDWNDPDNWSPVGVPGNCDQVYIPNGVPDNPIANTNLSI
                     GGLIIESGSFLDMNGYNLICKDTLYIDDGDISNANSIQFNKINDPYIFDASIQANTAI
                     TFDKYFGRLYFAYNTFIGNVILIDSIGRSGLAELSNNHFNNDLTIFGASQVPGAHTII
                     SGSGDDYISGSFGIAYTSVANLQVGTGGTLHIGGDLNLNTDVNPALINLNAVNFNGGN
                     NSYIRQFGTVPVSFNNLYTDKFSKAQLIIPEQPIYLRNVACLSGGLIKTSSAALLIAE
                     DNASINCHSSESWVWGPIKKIGNDQFTFVVGDSLKKALLTMATAPAQVTDAFTAQYFR
                     TNPSNAGYDTAQHVSSLTTVSGKEYWLFTRDNGNSNVRINLQYDSTRSQQISSIYSLR
                     ASQWNGSQWLNNGALSITGNLHEAYVTSQDVLSSFGPLTLGYVLPPRIPVITVTNIDT
                     IACRGSSFKVHFTVDTLMFPTNTFTAQLSDSTG"
     gene            3378..5348
                     /locus_tag="PK977_08750"
     CDS             3378..5348
                     /locus_tag="PK977_08750"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF18244.1"
                     /translation="MRKLHLLLLLLMISCPFIGLTQTVLADSLRHSVYAAKTQEDRLV
                     ALIDMFEEYHSINRDTLDTYGPVVKELAAASKNKRLKSLAELAYANWYYRWGWSDSSL
                     AFIEPEIARNPVDDPATRDIYFKLDRAKAMYLGSKSRFAEALDVLYGILPKAEQYNDT
                     LTIGLTCNTIGSIAIARQQLNEALKWINRAIVVAGNKGRHLQVLAPAYINAGYAYASL
                     GKMDSASYFIEMGLPICRQIQNLNYVATALRIQTNIYTATKQYKLAEKALLEMISTRA
                     KLTSANFIVEDNLQLADFYANSGQIDKAIEICKNNLKKGNIESTTNESNEVVSNDPKL
                     RVTFLEKLAGYYKQAGRLDEYRLSLEELVSAKDSLYAANSAEAIAELQTKYDVQKKEN
                     TILQQKYDLQQKNFLFYGSIILTGLLAVIAIALFRKYRKRQKAKVQLMMEEERKQAEE
                     AIKKAEEKERVRIASDLHDNLGAYAASMASNLSYIHLPDADERTNNALMQLANNSNAI
                     ISQLNDTIWVLKKDELSLTAISDRIKNFISRLRKSYPAVNIDVQENITIDHKLPSSQA
                     FHLYRIMQEAVNNALKHSSGENILINIAGGNKWEVTIADDGKGFNINEVKPNAGNGLS
                     NMMVRSSEAGWSVSWERSFPRGMVVKITSTTN"
     gene            5390..>6001
                     /locus_tag="PK977_08755"
     CDS             5390..>6001
                     /locus_tag="PK977_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18245.1"
                     /translation="MNFIVALCEDNNVNRNTFLQKIQVLGNMQVAFVAANGHDCLEQL
                     KGLPKAKLPQVIFMDLEMPHMNGIETIRLAKSLYPSVHFIVLTVFDDDEKIFEAIKSG
                     ASGYLLKHEPAGILQEAVMNVLEYGGAPMSPAIARKALQLMSKSILQGSEVKGTLPEM
                     ITSREEGVLKFMVNGWDAKRIASELDISVLTVRKHIANIYEKLH"
     assembly_gap    6002..6016
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6059..6508)
                     /locus_tag="PK977_08760"
     CDS             complement(6059..6508)
                     /locus_tag="PK977_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF18246.1"
                     /translation="MSIIVRRAVKEDCPRLMELVKELAIYEKAPNDVTVTLEHFTKSG
                     FGDNPVWWAFVAEENGYIYGFALYYIRYSTWKGQAMYLEDIIVTESSRGKGIGKLLMD
                     RLIIEARERELKRIVWQVLEWNEPAINFYKKYNADFDAEWVNCSISV"
     gene            complement(6515..>7159)
                     /locus_tag="PK977_08765"
     CDS             complement(6515..>7159)
                     /locus_tag="PK977_08765"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HRF18247.1"
                     /translation="AKARKDAKEAEAKAESNQKKGTQPSHAMKDYDGLYHHPGYGTAE
                     VMTTNDSLFMLVPGKKMWLRHYHYDVFQTLFIDNKEGIDTTYKDPFKIQFTMNEAGDI
                     NAIQMQFEDELKPMEFARKPRPKELSKDELKKYEGSYELGPGVEAKFYIKGEKTLYAF
                     IEGQPEYELVPTDKNTFVIKVLSGYSVKFEENEKGEVTAASFIQPNGTFKAKKK"
     assembly_gap    7160..7169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7170..8342)
                     /locus_tag="PK977_08770"
     CDS             complement(<7170..8342)
                     /locus_tag="PK977_08770"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF18248.1"
                     /translation="MRFTLVLLCSLTVSTLFGQKKTAVSSDPLAGIDTLLERILKERK
                     GAGFAVAVVNQNKILYAKGFGYRDYEKKLPVTPNTLFAIGSCSKPFTSLLIGLLQKDG
                     KVEYDKPVRDYLPELKFFNNEMNNNVTLRDMMSHRTGLPRHDYSWYLFNTSSRDSMLQ
                     RIQYQEPTAPLRQRWQYNNFMFLAQASVAEKLTGQSWEKNIKEKILVPLGMTASNVSI
                     AELKQSAEPSLGYTVYKDSIIKKTDYYDINAMSPAGSINSSVTDMAKWVMCWINGGKV
                     NGKEVFPAVYMKEAMSSQMITSAALPTKERQDVFISNYGFGWFLASYRGHYRVEHGGN
                     IDGFSASTSFFPTDSIGIIVLSNQNASAITGLVRNSIADRLLKEKPIDWLGERMAAD"
     assembly_gap    8355..8364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8365..9596)
                     /locus_tag="PK977_08775"
     CDS             complement(<8365..9596)
                     /locus_tag="PK977_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRF18249.1"
                     /translation="MNKSYPRQEDNLNNSLPLTGWGSAAVHAGHKKDPMYAHQVPIYA
                     SSTYVFDEAEQGMRRFSGKEEGYIYSRWGNPTITEAEEKIVALETFGLNMQAKGILHS
                     SGMAAITTLMLATLKPGDKILTHYSLYGGTNELVNKVLPDLNITTVIADLRELNKAAE
                     ILKTEPAIKMIYLETPANPTIQCVDIEELTRLGKQYKCIVACDNTFATPYLQQPFQYD
                     VDFIVHSTTKFLNGHGTAIGGTLLGKDLDFMNSHCTKVHRLLGGNSNSFDAFLLIQGM
                     KTLEVRMERHCHNAMEVANFLASHPAVAKVNYTGLSEHPDHHTAMKQMRHPGAMLSFE
                     LKSGLQGGINFMNKLKMCVRTVSLGTCDTLLSHPASMTHYSVPAAEKERYGITDGLIR
                     MNVGIENIQDIIADLKQA"
     gene            9665..>9916
                     /locus_tag="PK977_08780"
     CDS             9665..>9916
                     /locus_tag="PK977_08780"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF18250.1"
                     /translation="MKDLLKQYASYNVWANHRISEIILSLPEEKQQAEVVSSFPSLYK
                     TALHIWDAESGWWQRIKMQERVIFPRDSFSGSMQDLFTGL"
BASE COUNT         2848 a         1940 c         2062 g         2923 t
ORIGIN      
        1 gtatcctggt tggtggtaaa tgtaccgcca ccactcactg accagttata attaacacca
       61 gcttcttgct ggctgggata gtatttatgt atacctgcac tgatgcagcc gatgcccgga
      121 ccttcaatag aaaaattaac tgatggaatt gttttaacgg ttagtgcttt tgtattaatg
      181 cttgtatcag gtggtgcggt gccagttact ctaatcttat aagcnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccggtact gtctgatagc tgagcagtaa
      361 aagtatttgt agggaacatt aaagtgtcta cagtaaaatg tactttgaag gatgagccac
      421 gacaggcaat tgtatcaata ttggtgactg taattactgg tatccggggt ggtaatacat
      481 atcctaatgt aagtggtcca aatgatgata aaacatcctg tgaagtgaca taagcttcat
      541 gtagattgcc ggtaatggat aaagctccgt tgtttaacca ttgcgatcca ttccattggc
      601 tggccctcaa actataaatc gaagagatct gctggctgcg tgtactgtcg tattgaaggt
      661 ttatacggac attgctgtta ccattgtccc gggtaaatag ccaatattct tttccggaaa
      721 ctgttgtcag cgatgaaaca tgttgtgctg tatcataccc ggcattagac ggattggttc
      781 tgaagtattg tgctgtaaaa gcatcagtta cctgtgcagg tgctgtggcc atcgtcagta
      841 atgccttttt caggctatca cctacaacaa acgtaaactg gtcattcccg atttttttga
      901 taggccccca cacccagctt tcagaactat ggcaattaat ggaagcatta tcttctgcta
      961 tcagcaatgc agcagagctt gtttttatca accctcctga caggcacgcc acattacgca
     1021 ggtaaatggg ttgttcgggt ataattagtt gtgcttttga gaatttatcg gtatacagat
     1081 tgttaaacga aaccggtact gtaccaaact ggcgaatata ggagttattg cctccgttga
     1141 aattaacagc atttaagtta atcaatgcgg gattgacatc tgtattcagg ttcagatcac
     1201 caccgatatg cagtgtgcct cctgttccca cttgtaagtt ggcaaccgat gtataggcaa
     1261 tgccaaaaga tccagaaata taatcatcac ctgaccctga aataatggta tgggcaccgg
     1321 gaacctggct ggcaccaaag atggtgaggt cattgttaaa atgattattg cttaactcag
     1381 ccaggccgga tcttccgata ctgtctatta aaataacatt tccaatgaat gtattgtatg
     1441 caaaatacaa tctgccaaaa tatttgtcga aggtaattgc agtgttggcc tggatgctgg
     1501 catcaaaaat ataagggtca ttgattttat tgaattggat ggaatttgca ttggaaatat
     1561 ctccatcatc aatataaagt gtgtctttac aaataagatt atacccgttc atgtcaagaa
     1621 aacttccgct ttctatgata agtccgccaa tggaaagatt cgtgtttgca attgggttat
     1681 caggtacacc gttgggaata taaacctggt cacaatttcc aggaacacca acaggactcc
     1741 agttatcagg gtcgttccag tctgtgccat ttatacctgt ccatgttttt acaataccgc
     1801 ttgtacaacc aggtccgcag ccagcctgga tttcagtgat gcttgagcaa cccggtgcat
     1861 tgttttgaac aatgccaaaa cctccattat taagatagtt acataccgga tcaaatgaac
     1921 atacagataa ttgtggattg ctttcaattt gtattccttc agtattgttg tcctgaatat
     1981 caagactatt attcagaaaa gccagggatg taatattcgg attttcataa acccgtaaac
     2041 gtcttccaat cgtaatgata ttgcccagtc ccgtgagatc agtaagctgg ttgtttccat
     2101 ggaaggcaag atcgccgtta cttatggaag taatgttgct taaagcggat atatcagtca
     2161 gtacatcatt tgcccagagt gaaaagccgc cagggatatc tcctgaaacc tggttaaatc
     2221 cgtttaatgt ggtaagattt gggttgagcc agaaatagaa attaacacat ccggtaacat
     2281 tatcaagccc ggaaagagag gtaagaccgg tattgatcat ccatattgtt ccaaggttgg
     2341 tatttaggag gttattgctt aagcctgaaa gagaggtaag gttatccaat ccggaaaata
     2401 gaaatcccca tacatattcg aggttattaa ggcttaaaga tgttaatgaa ggaagccgtt
     2461 caagtcttaa atagccattg attgaatcaa gatttgaaag actgcttaaa ctaagaatcg
     2521 gtgagaatcg tatatccagg taaccgttta tcttttctac actttcaaga ccagttaaac
     2581 ttgttaaagc agtactgtca atcaacactc cgccaatatt ggtgatcccg ttgcttaagc
     2641 ccgacagggt atttaatgca ggaagtttat taatgatgat tgtacccagg tttgtcaggt
     2701 tatttaagcc aatgctggtt tgcaggaaat tatattgcag ttccagtgta tctcctaaag
     2761 aagtcagagc tgcaaaatcg ctcagtgagg taatagcagt atatgagata ttaagttttt
     2821 gcgaaacact ttctatttcg tctagtgaat caagctgtgt gatagccggg cttgctccta
     2881 gaccgttgat ctcgagaatc tgtacggaag tacaaccggg ataaagaatt ttgaagctgt
     2941 caatttgcgt ttgccgggtc agaataattg tatcacaaga tgcttccgga gcattcatat
     3001 tccggtttag gtttgaacga ctgtaaattg tatgggctga ggcccattgg ggtataatca
     3061 ccttgttctc cagttcactc atgttcactt tctgtgttgt ctttctttgt gacaaagcag
     3121 gttgtgaaag cactaaggca ttcagaatga gaaagtagat aagtctggtc atcagtcagt
     3181 ttttagtgtc gttttaactg caaaggcagt tttataatca gaaaatctgc aagatttaag
     3241 gatacgccgg cttaaaacgg gatggatttg acactataaa aattgcattt agcaggcctc
     3301 cggccaatac tcaatttgta gtactcactg gaaaatctgc cggaattgga atattttaga
     3361 agtatcaagt ccagggaatg agaaaacttc atcttttatt attgttatta atgatttcct
     3421 gtccttttat agggcttacc caaactgtat tggctgacag tctgcgacat tcagtttatg
     3481 cggcaaaaac acaagaagac aggctagtag cattaataga tatgtttgaa gagtatcata
     3541 gtattaaccg tgatacactg gatacgtatg gtccggtggt gaaagaactg gctgctgcca
     3601 gtaaaaacaa acgattaaaa agccttgctg aactggctta tgccaactgg tattaccggt
     3661 ggggctggag tgacagttct ctcgcattta tcgaacctga gattgcccgg aatcctgttg
     3721 atgacccggc aacaagagat atttacttca agcttgacag ggccaaggct atgtatcttg
     3781 gctcaaaatc aagatttgct gaggccctgg atgttttata cggtattttg cctaaagcag
     3841 aacaatataa cgataccctc accattggac tgacctgtaa tacaattggt tcaattgcaa
     3901 ttgccaggca acagctaaat gaagctttga aatggatcaa ccgtgctata gtggttgctg
     3961 gtaataaggg caggcattta caggtgttgg cgccggccta tatcaatgca ggttatgcct
     4021 atgcgtcatt gggcaaaatg gattctgcca gctatttcat tgaaatgggg ttgccaatat
     4081 gccggcaaat tcagaacctg aattatgttg ccaccgctct tagaatacaa acaaatattt
     4141 atacagcaac aaagcaatat aaacttgctg aaaaagcgct gctcgaaatg atcagtacga
     4201 gggcaaaact tacatcagca aattttattg tggaagataa tctgcagtta gccgatttct
     4261 acgcaaattc agggcaaata gataaggcca ttgagatatg caaaaacaat ttaaaaaaag
     4321 gtaacattga gtcgacaact aatgagtcaa atgaagtagt aagtaatgat ccaaaattga
     4381 gggtgacttt tcttgaaaaa ttggctggtt attataaaca ggcaggaaga ctggatgaat
     4441 acaggttgtc attagaagaa ttagtaagtg ccaaagattc tttatatgct gctaattcag
     4501 cagaagcaat agctgaattg caaacgaaat atgatgtaca aaagaaagaa aataccatac
     4561 ttcagcagaa atatgacctg cagcaaaaga attttctgtt ttacggttca attatcctaa
     4621 ctggtctttt agccgttatt gctattgctt tattcagaaa gtacaggaag aggcaaaaag
     4681 caaaagtgca actgatgatg gaagaggaaa gaaaacaagc tgaagaggcg ataaaaaaag
     4741 cagaagaaaa ggaaagggta cgcatcgcct cagatttgca tgataacctt ggtgcttatg
     4801 ctgcctccat ggcttccaat ctcagttata tacaccttcc ggatgctgat gaaagaacaa
     4861 ataatgcgtt gatgcagctg gctaataact caaatgctat tatctcacag ttaaatgata
     4921 ctatctgggt attaaagaag gatgaactga gcctgacagc cattagtgac agaataaaaa
     4981 atttcataag tcgcttgcga aaaagttacc ctgctgtaaa tatagacgta caggaaaata
     5041 tcaccattga tcataagctg ccttcatcac aggcttttca tttgtaccgt attatgcagg
     5101 aagctgtaaa taatgcactg aagcatagca gtggagaaaa tattctgatc aatatagccg
     5161 gggggaataa gtgggaagtc actatcgctg atgatggtaa agggtttaat ataaatgagg
     5221 ttaagccaaa cgcaggtaac ggattgtcaa atatgatggt aagaagtagt gaagcaggct
     5281 ggagcgtcag ttgggaaaga tcattcccaa ggggaatggt ggtaaaaatt acttctacta
     5341 caaattgatt attttttgga acagctgaat taataatttt tactcttata tgaattttat
     5401 tgtagccctt tgtgaggata ataatgtgaa ccgcaatacc tttttgcaaa aaattcaggt
     5461 gttgggcaat atgcaggtag cgtttgtagc agccaacggg catgattgcc tggaacagtt
     5521 gaaaggtttg ccaaaggcaa aactgcctca ggtgattttc atggacctgg aaatgccgca
     5581 tatgaatggt attgaaacaa taagactggc aaaatcactt tatccttcag ttcattttat
     5641 cgttttaacc gtatttgatg atgatgaaaa aatttttgaa gcaatcaaat ctggtgcatc
     5701 tggttacctc ttaaaacatg agcctgcagg tatattacag gaagcggtga tgaatgtact
     5761 ggaatacgga ggtgcaccca tgagccctgc tattgcccgt aaggcacttc aattaatgag
     5821 taagtcaata ttgcagggta gtgaggtaaa gggaacttta ccggaaatga ttacaagcag
     5881 agaagaaggt gttttgaagt ttatggtaaa cggctgggat gccaaaagaa tagcttcaga
     5941 attggatata agtgtcctta ctgtacgaaa acacatcgct aatatttatg aaaagctcca
     6001 tnnnnnnnnn nnnnnntgaa attatcagta tggcacatca gcaaaaatgg ttcaatcgct
     6061 aaacagatat tgaacagttc acccactctg catcaaaatc ggcattatat tttttgtaaa
     6121 agttaatagc aggttcgttc cactccagaa cctgccatac tattcgtttt aattctcttt
     6181 ccctggcttc aatgattaaa cggtccatta acagctttcc gatgccttta ccccttgatg
     6241 attcagtaac aataatatct tcaaggtaca ttgcctgccc tttccaggtg gagtagcgga
     6301 tatagtagag agcaaatccg tatatgtagc cgttctcttc tgctacaaag gcccaccaaa
     6361 ctgggttatc gccaaaacca ctttttgtaa agtgctccag tgtaactgtc acatcatttg
     6421 gagccttctc ataaatagcc agttccttaa ccagctccat taacctcgga cagtcctctt
     6481 ttactgcccg cctgacaatg atgctcatat agtattattt cttttttgct ttaaatgttc
     6541 cgtttggctg aataaagctt gccgcagtca cttcaccttt ctcattctct tcaaatttta
     6601 cactgtagcc ggaaaggact tttatcacaa atgtattttt atcggtaggc accaattcat
     6661 attctggctg gccttcaata aaagcataca atgtcttttc ccctttaata taaaatttag
     6721 cttcaacacc ggggcccagt tcatagctgc cttcgtattt tttcagttcg tctttgctta
     6781 attcttttgg ccgaggcttg cgggcaaact ccattggctt cagttcatct tcaaactgca
     6841 tttgtatggc attaatatcc ccggcttcat tcatagtaaa ctgaattttg aacgggtctt
     6901 tatatgtggt gtcaatccct tctttattat ctataaataa tgtttgaaaa acatcataat
     6961 gataatgccg gagccacatt ttttttccgg gtaccagcat aaaaagagaa tcattagtag
     7021 tcattacctc cgctgttcca tatccggggt gatgatacag gccatcataa tctttcattg
     7081 catgagatgg ttgtgtgcct ttcttctgat tgctctctgc tttggcttca gcttctttag
     7141 catcctttct ggccttggcn nnnnnnnnna tcagccgcca ttctttcacc caaccagtca
     7201 ataggttttt ctttcagcag cctgtcggca atactgttgc gcaccagccc ggtaatggct
     7261 gatgcattct ggttagaaag tacaataata ccaatgctgt cggttgggaa aaatgaagtg
     7321 ctggcggaaa aaccgtcaat attaccacca tgctctaccc ggtaatgtcc acgataggat
     7381 gccaggaacc atccaaagcc atagttggag ataaacacat cctgtctttc tttggtgggc
     7441 aaagcagcgc ttgttatcat ttgtgaactc atagcctcct tcatataaac tgcaggaaaa
     7501 acttcttttc catttacttt ccctccatta atccagcaca tgacccattt tgccatatct
     7561 gtcacactgc tgttgatgct gcccgcggga ctcatagcgt tgatgtcgta ataatcggtt
     7621 tttttaatga tgctgtcttt gtaaacggta taacccaaag agggctcagc tgattgtttt
     7681 agttctgcaa tagatacatt agaagctgtc atgcccagcg gtactaaaat tttctctttt
     7741 atatttttct cccagctctg gccggtaagt ttttctgcta ccgaggcctg tgccaggaac
     7801 ataaaattat tatactgcca gcgctgacgc aatggcgcag tgggctcctg gtattgtatt
     7861 cgctgcagca tactgtccct tgaactggta ttgaataaat accaggaata atcatgccga
     7921 ggcaggccgg tgcggtggct catcatatcc cgcagggtaa cgttattatt catctcgtta
     7981 ttaaaaaact ttagctccgg caagtaatct cttactggtt tatcatactc aacctttcca
     8041 tctttttgca gcaacccgat aagcaaggag gtaaagggtt tgctgcagga gccaatagca
     8101 aataatgtat tcggcgttac cggtaatttc ttttcataat cccggtaacc aaaacctttt
     8161 gcgtagagta ttttgttttg attaactacg gccaccgcaa agccagcgcc ttttctttct
     8221 tttaaaattc gttcaagtaa tgtatcaata ccggccagtg ggtcggaaga aacggcggtt
     8281 tttttctgtc caaacaaggt ggagaccgtt aagctgcaaa gcagtaccaa agtaaatcgc
     8341 atagttagtt atttnnnnnn nnnnaaggcc tgtttcagat cagcaataat atcctgtata
     8401 ttttcaattc ccacattcat ccgtatcagc ccgtcggtaa taccatatct ttctttttct
     8461 gctgcaggca cactgtaatg tgtcatagaa gccggatgag aaagcagtgt atcacaggtt
     8521 cccaatgaaa ctgttcttac gcacattttc agtttattca tgaagtttat gcctccctgt
     8581 aatccactct ttaattcaaa gctaagcata gcaccgggat gccgcatttg tttcatggcg
     8641 gtatgatgat cgggatgttc agaaagaccg gtgtaattga cttttgcaac tgccggatgt
     8701 gaagcgagaa agttggccac ttccatcgca ttatggcaat gtctttccat tcttacttcc
     8761 agtgttttca taccctgaat caacaggaag gcatcaaagg aatttgaatt gccccccaac
     8821 aagcgatgca cttttgtaca atgcgagttc ataaaatcga ggtctttacc gagaagggtt
     8881 ccaccaatag ccgttccgtg accgttcaaa aatttagtag tagagtgaac aataaaatca
     8941 acatcatatt gaaacggctg ttgcaggtac ggcgtggcaa atgtattatc gcaggctact
     9001 atacatttgt attgcttgcc cagccttgta agctcttcta tatcgacaca ctgtatggtt
     9061 ggattggcag gcgtttctaa atatatcatt ttaatggccg gctccgtttt caatatttca
     9121 gctgctttgt tcagttcccg caggtcggca attacggtag ttatgttcag gtctggcaga
     9181 actttattca ccagttcatt ggtgcccccg taaagtgaat aatgagtaag aattttatca
     9241 ccgggtttta gtgtagccag catcagtgtg gtaattgcag ccatccctga agaatggaga
     9301 atgccttttg cctgcatatt caatccaaag gtttcaaggg caacgatttt ttcttccgct
     9361 tcagttattg tcggattgcc ccaacggctg tagatataac cttcttcctt cccactgaac
     9421 cggcgcatac cctgttctgc ttcatcaaaa acataagtac tactggcata gatgggcacc
     9481 tggtgtgcat acatcgggtc ttttttgtgc ccggcatgta cagcagcaga cccccagcct
     9541 gttaagggca atgagttatt cagattgtcc tcctgtcggg gataactttt attcatgcaa
     9601 aaaggttgta tttaagaaat ttggaaagat tcaaaaatac tgttttcaac cgcaccaacc
     9661 gccgatgaaa gatctgttaa aacaatatgc ttcgtataat gtatgggcca accaccgcat
     9721 cagcgaaata atactttcct tgccggaaga aaagcagcag gctgaagtag tttccagttt
     9781 tcccagtttg tataaaaccg cattgcatat atgggatgcc gaaagtggtt ggtggcagcg
     9841 tataaaaatg caggaaaggg tcatttttcc aagagatagc ttttccggct ccatgcagga
     9901 cctttttaca gggctt
//
LOCUS       DAORLS010000096         9900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3674_length_9900_cov_2.414017, whole genome shotgun sequence.
ACCESSION   DAORLS010000096 DAORLS010000000
VERSION     DAORLS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3674_length_9900_cov_2.414017"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="PK977_08785"
     CDS             <1..1519
                     /locus_tag="PK977_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18251.1"
                     /translation="QDIFFAKYDASGNYVYAKTLGGTSSDFGYGIAVDGSSNVYVTGV
                     FFGTGDFDPGAGTANLISSGNQDIFFAKYDASGNYVYAKALGGTSTDFSLAMALDGSG
                     NLYVTGYFSGTADFDPSTGVASLAANNPEDIFFTKYTGCSTPQVATVNRTASPAAISG
                     TTTFTDGGCSLVAVVQPNGASPVSGTVNAQVWIESSVPTVATQPFVARHYEITPATNP
                     STATGRVTLYFTQQEFTDFNNDPGSILDLPTGPADAAGKANLRIEKRNGTSSNGTGLP
                     NTYSGSITTIDPADADIVWNAALSRWEVSFDVTGFSGFFVKTTPSVLPLTLLEFNGRL
                     QNGDALLNWKTENEVNTDKFLVERSTDGRNFSSIGTVAARNQPGANLYNYTDAGVNTL
                     AASMVYYRLKQTDIDSRFTYSRIITLPLGKQNQLLLYPNPASDQLMITVSAERVEKVQ
                     VRIIDNAGRILRQQHWNIAAGSATIQLEVSTLAKGLYYLEVKGETTDQRKTFVKQ"
     gene            complement(1593..3266)
                     /locus_tag="PK977_08790"
     CDS             complement(1593..3266)
                     /locus_tag="PK977_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF18252.1"
                     /translation="MRSTKSIYLVTLQKESISPFAKESDEEKVKEPADSSKTKTVTDK
                     KESGLVIDWDGIQNRIVDLPVPAGNYSSLGMGKEGELLYIRNNPGGPGMMMKYDLKKR
                     KESEVSAMNGYILSADGKKMLYNAGGSWFISGSGEKGEPGKGMINLNDVQVKMDPKDE
                     WPQIFDEAWRINRDYFYDPGMHGANWAAMKKKYEVFLPHLSCRRDLNVLIQWLCSELA
                     VGHHRITSPGDRLNNPARVGGGLLGADYSVDKNRYRIKKIYGGLNWNPNLRSPLTEPG
                     VNAKEGDYILAVNGQDVTAADNFFSFFENTAGKIVELTIGPNADKTNSRVVKVVPVAN
                     ESALRNRDWVEGNLKKVTEATKGQVAYVYVPNTAALGHEYFKRYFFPQANRKAIIVDE
                     RFNGGGQLADYYIDLLQRPHQAHWNMRYGKDLKSPSASIQGPKVMLIDETAGSGGDML
                     PWMFRKFNVGTLVGKRTWGGLVGILGFPEFMDGGGVTAPNVAIWTKDGFVVENAGVPP
                     DVEIEQTPADVIKGRDPQLEKAIEIALKELEKNPQPDPKRPPYPNRVKN"
     gene            complement(3296..4840)
                     /locus_tag="PK977_08795"
     CDS             complement(3296..4840)
                     /locus_tag="PK977_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18253.1"
                     /translation="MQLSKKNLLSISFLSIVTFINASPIDPEDTRMLSQPAVSNSHIC
                     FMYAEDLWVANRDGSNPRRLTVDEGTESNPVFSPDGKWIAFTGDYDGNTDVFIVSADG
                     GIPTRLTWHPSPDLVRGFSPDGKSVLFLSPRNAFNNRYAQLYTVPVGGGIETQLVIPN
                     ANWAAYSPDGKQMAYTPLSDAFTQWKHYRGGTIATIIVFSFADNSSYKIPQPAGGCND
                     VNPVWAGNMIYFRSDRNGEFNIFSFNTATKEVKQVTTFKDFPVLYLSGNGNSLIFEQA
                     GYLHSLTPGENSKRLKVGIATDLQELRSRYAKGGSYVRSADISPTGQRIVVDFRGDII
                     TLPAEKGDERNITQTTSVHEKYPAWSPDGKNIAYFSDASGEYQLHIKAQDGKGDAKAY
                     KLNGTGFYANTKWSPDSKKITFTDNGRNLYLLDVASGAIDKIDADELYVPGAFRNQFG
                     DWSSDSKWMAYTKVTETQFKKIYLYSVEQKKSFALTDGLSDATDPVFDKGNKYLFFFA
                     STDAGL"
     gene            complement(4948..5571)
                     /locus_tag="PK977_08800"
     CDS             complement(4948..5571)
                     /locus_tag="PK977_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18254.1"
                     /translation="MDKIKVLITDDHPMVLEGMKSMLQQIRFVEITGMASNAYECMDQ
                     IKNNTPHIVITDINMPEISGIELTQKIRKEFPQVKVIAMSTFKERSYISQMIQNGASG
                     YLVKSASKEEIEEAILSVYEGKLYMSLDINLSTAEKNELNNIPVLSSREKEVLLLIAD
                     GFTNPQIAEKLFISLHTVDSHRKNLLTKFGVNNTAGLIKLATRFNMV"
     gene            complement(5564..7501)
                     /locus_tag="PK977_08805"
     CDS             complement(5564..7501)
                     /locus_tag="PK977_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF18255.1"
                     /translation="MWKAFLISAAMLTCFVQGRSQPGQVTDSLLNQLRSAKEDTNKVL
                     LLISIGQQTENSNPQVAKNYYLQAKALSKKLNYAAGEIKFYLNYTAVLNLQSHFDSSL
                     VLNLESVELAKKSGSPLDIAKCIQNVGVSWYYLQDYEKSTEHYLQAVPYFEKAKDTFR
                     LSILYSNIGLVFREAKLFDKALEYHQKALKLSRQLNDPYETGLTLTNIGIVYSDLKQY
                     GKAEAVLTEALQLARQTNDDYLMEQVLLNITNLYLKKREYPQVKNYAEGALQYSKKLG
                     DSLVIAVSLYSLAQEAFHRSDFSAASSFASEGLAIAAQKNFKEQQEILYEFMASLALV
                     KKDIAAYTRHKGVADSLSEILLAETVQKNVQQLDKKFETEKKNNEIIQLARDKKVKAL
                     WNYLLAGIAGSLIIISLFAYRTYRQRQKLQEQQIAELEKEKQLLATQSLLKGQEEERS
                     RMAKDLHDGLGGLLSGVKLQLGAMKGNLILSEEHGRIFTNALNKLDASIHEMRRVAHN
                     MMPEALMKLGLQQALQDYCDGLAEGQPFTINSEFYGLENRMDPSTEVVIYRIVQELVN
                     NAVKHSGASSILAQVMRHNNNLTITVEDNGKGFDKEAMAHGSCLKNVRSRVDYLKGNL
                     DIKTMPGQGASIHIDCIISNG"
     gene            7633..>8904
                     /locus_tag="PK977_08810"
     CDS             7633..>8904
                     /locus_tag="PK977_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18256.1"
                     /translation="MKRLFTPLLFVIAFAVNGNAQCWQQVSSGPLFSLGIKTDGTLWA
                     WGLNTDGQLGVGNTTSYNYPVQVGTSNDWLLVSAGYDRVHAIKTDFTLWVWGNNNNGK
                     LGTGSYGFVYSPVQIGTGNDWQSVAAGGSHSMAVKWDGTLWGWGDNFYGQVGITGAPN
                     YTTPQQVGTDNDWRMVSAGFGHTMAIKSNNSLWACGYNEFGQLGTGDNNNKNILTQVG
                     VAEWQTAEASYLSSHLIKTNNTIWSCGRNDFGRLGNGNNTDSNVPVQEITLATDWASV
                     SSGQTLVLAQKNDGSLWSWGYGPNGASYINQNTPAQAGTETDWTQVSVTWNHTLAIKT
                     GNTAWAWGDPTEAGELGNGTNTTSTSPVQVSCLAILPVTWLFVNGQLQNGNAIIKWAT
                     ASESNTDKFEIEHSSNGINYNKVGTRAATGNS"
     assembly_gap    8905..8950
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8951..>9160
                     /locus_tag="PK977_08815"
     CDS             <8951..>9160
                     /locus_tag="PK977_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18257.1"
                     /translation="VNGKNFYRIKQIDLDGGFTYSSIIVLQNNDTRTKAIIAPNPVIN
                     TLSLFFSETGTKTIQLVSMDGRLLLT"
     assembly_gap    9161..9184
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9359..>9900
                     /locus_tag="PK977_08820"
     CDS             9359..>9900
                     /locus_tag="PK977_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18258.1"
                     /translation="MKKTITIPFALFFSITMLGQKINNYDIFSFTPPKGYKIMEQGKR
                     VLYQQKDDSTFCQIHLWPAQQGSSDPQANFKTDWDYFAGNQYKIGDPANKQNEKMGGW
                     DVVTGVGVAAVEGMQFMVSVSTFTQKDLSWCIVTLFNDEKYTSDIDAFVGSIVTDVTK
                     FKRTNTTQQQPAHTAQNQQN"
BASE COUNT         2656 a         2245 c         2171 g         2758 t
ORIGIN      
        1 tcaggatatc ttttttgcca aatacgatgc cagcggcaat tatgtatatg ccaaaaccct
       61 gggcggaaca agcagtgatt ttggctatgg tatagcggta gatggcagca gcaatgtata
      121 tgttactggt gttttctttg gcaccgggga ctttgatccc ggtgctggta cagccaatct
      181 tattagttca ggaaatcagg atatattttt tgccaaatac gatgccagtg gtaattatgt
      241 atatgccaaa gccctgggcg gaacaagcac tgatttttcc ttagctatgg cactggatgg
      301 cagcggcaat ttgtatgtta caggatattt tagcggaaca gcagactttg accccagtac
      361 aggcgtagct tctcttgctg caaataatcc agaagatatt ttctttacca aatacaccgg
      421 ctgcagtacc ccccaggtgg caacggtaaa ccgtacggcc agcccggctg ccatatccgg
      481 cactaccacc tttaccgatg gtggttgcag cctggtggct gtggtgcaac ccaacggtgc
      541 aagcccggtc agcggaacag tcaatgcaca ggtgtggatt gaaagcagtg tacccaccgt
      601 tgccactcag ccctttgtag ccaggcacta tgaaataaca ccagctacca atccttctac
      661 cgctactggc agggtcactt tatatttcac gcaacaggag tttactgatt tcaataacga
      721 tcccggcagt atactggatc tgccaacggg tcctgccgat gcagcgggta aagccaatct
      781 tcgtattgag aaaagaaacg gaaccagcag caatggtacc ggtctgccca atacctacag
      841 tggcagcatt actaccatcg accctgccga tgccgatatt gtatggaatg cagcccttag
      901 ccgctgggag gtaagctttg atgtgacagg ctttagcggc ttctttgtaa aaacaacgcc
      961 ttctgtatta ccactcaccc tgctggagtt taatggcagg ctgcaaaatg gtgatgctct
     1021 gctgaactgg aaaacagaaa atgaagtgaa caccgataaa ttccttgttg aaagaagcac
     1081 tgacggtaga aacttctcaa gtataggtac tgtagctgcc agaaaccagc ccggcgccaa
     1141 tctgtacaac tataccgatg caggtgtaaa tacattagca gcatccatgg tttactaccg
     1201 gctgaagcaa acggatatag acagccgctt tacctattcc cgcattatta cgttgccgct
     1261 gggcaagcaa aaccagttat tgctttatcc caaccctgcc agcgatcagc tgatgattac
     1321 cgtatctgca gaacgggtag agaaagtaca ggtaaggata atagacaatg ccggaagaat
     1381 attgcggcag caacattgga atattgcagc aggcagcgct actatacaat tagaagtaag
     1441 cactcttgcc aaaggattgt actacctgga agtaaaaggt gaaactactg accagcgaaa
     1501 gacctttgta aaacaataag cgatcatgaa aagcccttcc ataaaaaagc ccctgcactg
     1561 caggggcttt tactattacc attttattct tatcaatttt tcaccctgtt cggatagggt
     1621 ggccgctttg gatctggctg cgggttcttc tctaattctt tcagggcaat ttcaattgct
     1681 ttttccagtt gcgggtctct tcccttaatt acatcagcag gtgtttgttc tatttccaca
     1741 tcgggtggca cacctgcatt ctctactaca aaaccatctt ttgtccagat agctacgtta
     1801 ggcgctgtta caccgccacc atccatgaac tcgggaaaac cgagtatgcc caccaggccg
     1861 ccccatgttc gtttgcctac cagtgtacct acattgaatt tgcggaacat ccatggcagc
     1921 atatcaccac cggagccggc agtttcatcg atcagcatca ccttcggtcc ctgtatagag
     1981 gcgctgggcg atttcaggtc tttgccatac cgcatattcc agtgtgcctg gtgcggacgc
     2041 tgcaacaaat caatatagta atcggctaac tggccgcctc cgttgaagcg ttcatctacg
     2101 atgatggctt tgcggttggc ctgcgggaag aaataacgtt tgaaatattc atgacccagt
     2161 gccgcagtgt tgggcacata cacataggcc acttgtccct tggtggcttc ggtcactttt
     2221 ttcaggttgc cttccaccca gtcacggttg cgcagcgccg attcattggc cacaggcact
     2281 accttcacta ctcttgagtt ggttttatct gcattggggc cgatcgttaa ctctacaatt
     2341 ttaccagcgg tgttttcaaa gaagctgaag aaattatctg cagccgttac atcctggcca
     2401 tttacagcaa ggatataatc accttccttt gcattcacac cgggctcggt caatggtgaa
     2461 cgcaggttag gattccagtt cagcccgccg tatatttttt taatgcggta acgatttttg
     2521 tcaacgctgt aatcggcgcc gagcaaacca ccgcccactc ttgcaggatt gttcagcctg
     2581 tcgccgggag aggtgatgcg gtgatgacct acggccagtt cactgcatag ccattgtatc
     2641 agaacgttca ggtcacggcg gcaggaaaga tgcggcagaa acacttcata ctttttcttc
     2701 attgctgccc agttggcgcc gtgcataccg ggatcataga agtaatccct gttgatgcgc
     2761 catgcttcat caaatatctg cggccattcg tctttcgggt ccatcttcac ctgtacatca
     2821 tttaagttga tcatgccctt gcccggttct cctttttcac cggagccgct gatgaaccag
     2881 ctgcccccgg cattgtacag catcttcttg ccatcggcag aaagtatata accattcatc
     2941 gcagatacct cactctcctt tctcttcttc aggtcgtact tcatcatcat acccggaccg
     3001 cccgggttgt tgcggatata gagcagctct ccttctttac ccataccgag tgaagaatag
     3061 ttgccggccg gaacgggcag atcaacaatt cggttttgaa taccgtccca gtcaatcacc
     3121 agtccgctct ctttcttatc agtaacagtt ttggttttag atgaatcagc cggttctttt
     3181 actttttctt catcgctttc cttggcaaaa ggtgaaatgg attctttctg cagtgttacc
     3241 aggtaaatgg atttggttga ccgcatatca ttgttcgact ggtcgaacca gttcactaca
     3301 ggcccgcatc ggtagaagca aagaagaaaa ggtatttatt gcccttgtca aataccgggt
     3361 cggtagcatc actcagcccg tcggtaagag caaaggattt tttctgctcc accgagtaga
     3421 gatatatttt cttgaactgt gtttccgtca ctttcgtata cgccatccat ttcgaatcag
     3481 aagaccagtc gccaaactgg ttgcggaagg cgccgggtac atacagttca tcggcgtcta
     3541 tcttatcaat ggcgccgctc gccacatcaa gtaaataaag atttcggcca ttgtctgtaa
     3601 aagttatttt tttactgtca ggcgaccatt ttgtattggc ataaaaacca gtaccgttca
     3661 gtttgtatgc tttggcatca ccctttccat cctgtgcttt gatgtgcagc tggtattcac
     3721 cgctggcatc agagaagtaa gcaatatttt ttccatctgg tgaccaggca ggatattttt
     3781 catgtacaga agtggtttgg gtaatgttgc gttcatctcc tttttcggca ggcagggtaa
     3841 tgatgtcgcc gcggaaatcc accacgatac gctgaccggt gggagaaata tcagcactgc
     3901 gtacataact gccgcccttt gcatagcggg aacgcagttc ctgcagatcg gtggcgatgc
     3961 ccactttcag ccgcttgcta ttttcgccgg gagtaaggct gtgcaggtag cccgcctgtt
     4021 cgaagatgag gctgttgcca ttgccggaca ggtatagcac ggggaagtct ttaaacgttg
     4081 taacctgttt tacttctttg gtagccgtgt taaaagaaaa gatattgaac tcaccgttgc
     4141 ggtcgctgcg gaagtagatc atattgcctg cccataccgg gttcacatca ttacaaccac
     4201 cggcaggctg tggtatttta taactgctgt tgtcggcaaa actgaaaacg atgatagtag
     4261 caatagtgcc gccgcggtaa tgcttccact gggtgaaggc atcgctcagc ggagtataag
     4321 ccatctgctt gccatcgggt gagtaagccg cccagttggc attgggtatt accaattgtg
     4381 tttcgatgcc gccgcctaca ggaacggtat atagttgtgc atagcggttg ttaaaagcat
     4441 tgcggggaga aagaaataac actgatttgc catccggtga aaagccacga acaagatccg
     4501 gcgatggatg ccaggtaagc cttgtgggaa taccaccatc cgcggaaaca atgaatacat
     4561 ctgtgttgcc atcataatca ccggtgaagg cgatccattt gccatcggga gaaaaaacag
     4621 gattgctttc tgtgccttca tctacggtaa ggcggcgggg attagatcca tcccggttgg
     4681 ccacccacag gtcttcggca tacatgaagc agatatggct gttgctgaca gccggctgtg
     4741 acaacatacg ggtatcttcc gggtcaatcg gtgaggcatt aataaaagtt acgattgaca
     4801 aaaaagaaat agataataaa tttttttttg atagttgcat aacgaatgtt ttgcagatga
     4861 aatgaatggc gggacggaaa agaaagagta agttagtgca tttcgaggag acctaaacag
     4921 ggtgtccctg gtgattttct gcagtgatta caccatattg aaacgggtag ccagtttaat
     4981 gagacccgcc gtgttgttaa caccaaattt ggtcagcagg tttttacggt ggctatccac
     5041 tgtatgcagg ctgataaaaa gtttctctgc aatttgtgga ttcgtaaatc cgtcagcaat
     5101 caaaagcagc acttcttttt cccggctgct gagtaccggg atattattga gttcattttt
     5161 ttcagcggta gaaagattga tgtcaagact catgtacagt ttcccttcat acacactcaa
     5221 tatggcttct tcaatttctt cttttgaagc actctttacc aaataaccag aagctccgtt
     5281 ttgtatcatt tgtgaaatat agctccgctc tttaaaagtg ctcatggcaa ttacttttac
     5341 ctgcgggaac tcttttctta ttttctgtgt aagctctatt ccgcttattt ccggcatatt
     5401 gatatcggtg ataacaatat gcggggtatt attttttatt tgatccatgc actcgtaagc
     5461 attgcttgcc ataccggtaa tttcaacaaa acgtatttgc tgcagcatac tcttcatgcc
     5521 ttcaagcacc atcgggtggt catctgttat caatactttt attttatcca ttgctgataa
     5581 tacaatcaat atgtatggat gctccctggc ccggcatggt ctttatatct aaattccctt
     5641 tcagataatc tacccgggag cggacatttt ttaaacacga gccatgtgcc attgcttcct
     5701 tgtcaaaacc ctttccattg tcttcaacag tgatggttaa attattatta tgccgcatca
     5761 cctgtgccag tatggatgaa gcccccgaat gttttactgc attatttact aattcttgta
     5821 caatacgata gatcactact tcggtactgg ggtccatccg gttttccagt ccataaaact
     5881 cgctgttaat ggtaaagggc tggccttcag caaggccatc acaataatcc tgtagcgcct
     5941 gctgcaagcc cagtttcatt aacgcttcag gcatcatatt atgggctacc cttcgcattt
     6001 catgaataga agcatccagc ttgttcagcg cattggtaaa aatgcggcca tgttcttccg
     6061 ataaaataag gtttcctttc atagctccta attgaagttt aacaccactt aataatccgc
     6121 cgaggccgtc atgcaggtct ttggccatac ggcttcgttc ttcttcctgt cctttaagca
     6181 gtgattgggt ggcaagtaat tgtttttctt tttccagttc ggcaatttgt tgttcttgca
     6241 gtttttgccg ctgccggtaa gtccggtaag caaacaacga aataatgatc agcgaaccag
     6301 caataccggc aagaagataa ttccagagag cttttacttt tttatccctg gccagttgaa
     6361 ttatttcatt gttctttttt tctgtttcaa attttttatc cagttgttgt acattttttt
     6421 gaacagtttc agccagtaat atttcactca aagagtcagc caccccttta tgacgggtat
     6481 aggcagcaat atcttttttc acaagagcaa gagaggccat aaattcatac agaatctcct
     6541 gctgctcttt aaagttcttc tgagctgcaa tggcaagtcc ttcactggca aagctgctgg
     6601 cagcagaaaa atcacttctg tgaaaagctt cctgcgcaag agagtacaga gaaacagcaa
     6661 tcacgaggct gtcgcccagt tttttgctgt attgtaatgc tccttcagca taatttttta
     6721 cttgcggata ctctctcttt ttcaagtaca ggtttgtaat attgagcaac acctgctcca
     6781 tcagataatc atcatttgtt tgccgggcca gttgcagcgc ttcggttaat acagcctctg
     6841 ctttaccata ctgcttcagg tcagagtata caatcccgat attggttaac gtaagccctg
     6901 tttcatacgg atcattcagt tggcgtgata gtttcaaagc tttttgatga tactccagtg
     6961 ccttatcaaa taatttagct tcacggaata caagcccgat attgctgtac aagatagaca
     7021 gccgaaaagt atcctttgct ttttcaaaat agggaacagc ctgcagataa tgttccgtac
     7081 ttttttcata atcctgcaaa taataccagg atacacctac attctgaata cacttggcaa
     7141 tatccagggg gcttcctgat ttttttgcca gttcaaccga ttcgaggtta agtaccaggc
     7201 tggaatcaaa atggctctgc aggtttaata cagcagtata attgagataa aactttattt
     7261 ctccggcagc ataattaagc tttttactta atgcctttgc ctgcaggtaa taattctttg
     7321 caacctgcgg attactgttc tctgtttgct gaccgatact gatcagcagc aatactttat
     7381 tggtatcttc tttggcgctc cgcaattgat ttagcaagct gtcggttact tgccccggct
     7441 gagaccggcc ctgaacaaag caggtaagca tagcggctga tataagaaat gctttccaca
     7501 tcactttatg caaatatgat aaaaatacct gcaagacctc atccccagaa caggggattc
     7561 taaaagcagg tattcggggg attgatccac ctgcttatta tttgaagttt tgtattcata
     7621 aaatcagcat ttatgaaaag attatttact ccactgctct ttgtcatagc atttgcagta
     7681 aatggtaatg cacaatgctg gcaacaagtt tcctccggac ccttgttttc tttaggcatc
     7741 aaaacagatg gcactctctg ggcctggggg ctgaacaccg acgggcaatt gggcgttgga
     7801 aacacaacga gctataacta tccagttcag gtaggtacca gtaatgactg gctgttggta
     7861 tcagcaggat acgatagggt gcatgctata aaaacagatt ttacattgtg ggtctgggga
     7921 aataataata acggcaaact gggcaccgga agctatggct ttgtttattc tccggtacaa
     7981 attggaactg gtaacgactg gcaatcggtg gccgcaggcg gaagccattc aatggctgtt
     8041 aaatgggatg gtactttgtg gggctggggc gataactttt atgggcaggt tggtattacc
     8101 ggggcaccca attacactac tccgcagcag gtaggtacag acaacgactg gcgaatggtt
     8161 tctgccggat tcgggcatac aatggctata aaatcaaata acagcctatg ggcctgcggc
     8221 tataatgaat ttggacagct gggtacaggc gataacaaca acaaaaatat ccttacacaa
     8281 gtaggcgttg ctgaatggca aacggcagaa gcaagttatt taagttcaca cctgataaaa
     8341 acaaataaca ctatctggag ttgtggcaga aacgattttg gcaggctggg caacggtaat
     8401 aatacagaca gcaatgtacc tgtacaggaa attacattgg ctacagactg ggcctctgtc
     8461 tcaagtggcc agacattagt gcttgcacaa aaaaatgacg gaagtctctg gagctggggc
     8521 tatgggccta atggagcatc ttatattaat caaaatacac ctgctcaggc aggtactgaa
     8581 acagactgga cacaggtgtc agtaacctgg aaccatacac ttgccataaa aacaggtaat
     8641 acagcctggg catggggaga cccgactgaa gcaggcgaat tgggtaacgg aacaaatacc
     8701 acttctacta gtcctgttca ggtaagttgc ctggcaattc ttcctgttac ctggctcttt
     8761 gtaaacggtc aattgcaaaa tggcaatgca attatcaaat gggccactgc atctgaaagc
     8821 aataccgata aatttgaaat tgaacacagc agcaatggaa ttaattataa caaagttgga
     8881 accagggctg ccacgggtaa cagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn gtaaacggaa aaaactttta ccgcataaaa caaattgacc tggacggagg
     9001 atttacttat tcctccataa ttgtattaca aaacaatgat accagaacaa aggccatcat
     9061 agcgcctaat ccagtgataa acaccctgtc gttgttcttt agtgaaacag gcactaaaac
     9121 cattcaactg gtaagtatgg atggccgttt attgcttaca nnnnnnnnnn nnnnnnnnnn
     9181 nnnncagtac ccacaccatc aatatgcagc cactgcctgc tggtatgtat gtgctgcgct
     9241 tgattaccag caaaggaaca gatacttata aaatcaataa acaataaacg ctattaatat
     9301 gaaaagcaat aagccgttat tcatcatttt tgtaaaataa ttttttaact aacctataat
     9361 gaaaaaaaca ataaccatac ccttcgcctt atttttttca ataacaatgt tggggcaaaa
     9421 gataaataat tacgacatat tttctttcac tccgcccaaa ggatataaga taatggagca
     9481 gggcaaacgg gttttgtacc agcaaaaaga tgacagtacc ttttgtcaaa ttcatctttg
     9541 gcctgcacaa caaggctcat cagacccgca ggcaaatttt aaaaccgact gggattattt
     9601 tgccggtaat caatataaaa tcggcgaccc ggccaataag caaaatgaaa aaatgggagg
     9661 atgggatgtg gttaccggtg tgggtgtggc cgcggtggag ggaatgcagt tcatggtctc
     9721 tgtttctact ttcacacaaa aagatttgag ctggtgcatt gtaaccctgt ttaatgatga
     9781 gaaatacact tcggatattg atgcttttgt tggttccatt gtaacagatg taacaaaatt
     9841 caaaagaacg aataccactc agcagcaacc agcccatact gcacaaaacc agcaaaacaa
//
LOCUS       DAORLS010000097         9862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3692_length_9862_cov_1.665851, whole genome shotgun sequence.
ACCESSION   DAORLS010000097 DAORLS010000000
VERSION     DAORLS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3692_length_9862_cov_1.665851"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK977_08825"
     CDS             complement(<1..249)
                     /locus_tag="PK977_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HRF18259.1"
                     /translation="MTKEQILDALRNVQEPDLGKDLVTLNMVQDIEINGNDVSFTIVL
                     TTPACPMKDMMGNASTNAIKLLVNKEANVKVNFTSNTTT"
     gene            351..992
                     /locus_tag="PK977_08830"
     CDS             351..992
                     /locus_tag="PK977_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF18260.1"
                     /translation="MNKFLLYTGLLILLTTQTAKAQFETSRDSVVQLYGVVMTADSLV
                     GIPAVSIMVKGQNRGTITNAQGVFSIVVLKGDQVEFSHVTYKSKMVTIPRTLEGNQYS
                     MVQLMVVDTVYLPATIIKPRPTPEQFARDFVNVKVPDDDIEIARRNTNAANRRAWLRT
                     IPGDGGEATRQQFNKIANRAVYQGQTPPQNIFNPAAWAEFIQAWKRGDFKNKK"
     gene            1281..>1733
                     /locus_tag="PK977_08835"
     CDS             1281..>1733
                     /locus_tag="PK977_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRF18261.1"
                     /translation="MNIKGGISVIGAGTMGNGIAHVFAQKGYKVTLIDISQAQLDKAI
                     ATIGKNLDRMVAKETITSEAKEETLKNISTNTSIEEGVKDAALVVEAATENVTLKLQI
                     IQQIDKAAPADCILATNTSSISITKIAAATKRPEKVIGMHFMNPVPVMK"
     assembly_gap    1734..1870
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1871..2182
                     /locus_tag="PK977_08840"
     CDS             <1871..2182
                     /locus_tag="PK977_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRF18262.1"
                     /translation="MPMINEAIYSLYEGVAGVEEIDTVMKLGMAHPMGPLQLADFIGL
                     DVCLAILNVLHDGFGNPKYAPCPLLVNMVTAGKLGAKSGEGFYLHTPGSKELVVSGRF
                     K"
     gene            2199..>2575
                     /locus_tag="PK977_08845"
     CDS             2199..>2575
                     /locus_tag="PK977_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18263.1"
                     /translation="MKRCLITLVIVTSTIIACKNNPQKTTIEQNVSNDSTSISIDWDS
                     SYIITSSQAFRTDNGFELQIDGLNIDFQNRENRWFASINQVALADCLFVSSQFRMLNQ
                     SIKLNKDQVDIGDKLWGFIELLTL"
     assembly_gap    2576..2678
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2888..2897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2898..3372)
                     /locus_tag="PK977_08850"
     CDS             complement(<2898..3372)
                     /locus_tag="PK977_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRF18264.1"
                     /translation="MAAAVNQPEYFKTIETTAMAEFRDKGSKFIAFTFPVSDVNDFKA
                     RLADIKKEHPKATHHCFAYRIGLDGTNFRVSDDGEPSGSAGRPILGQIDSKQLTNVLI
                     IVVRYFGGTLLGVPGLINAYKSAAAMALQVTPVLQKPVLVNYRIQFDYTQMNEVMK"
     gene            complement(3347..3592)
                     /locus_tag="PK977_08855"
     CDS             complement(3347..3592)
                     /locus_tag="PK977_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRF18265.1"
                     /translation="MNWIVWIGYMAAALVVISFLVSKNMRLLRTINMLGAALFILYGV
                     LLNVNLPVIIPNAVIVGIQVYYLFIKREESGRSSQPA"
     gene            complement(3598..4674)
                     /gene="ribD"
                     /locus_tag="PK977_08860"
     CDS             complement(3598..4674)
                     /gene="ribD"
                     /locus_tag="PK977_08860"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRF18266.1"
                     /translation="MTIDQSFMHRCLELAKLGAGHVAPNPMVGAVLVHEGRIIGEGWH
                     KEYGKAHAEVNCINAVKESDRQFISQSTLYVSLEPCAHYGKTPPCTDLIIRYNIHRVV
                     IGCRDPFKEVDGKGIEKLKVAGIEVETAVLEQECTELNKRFFTFHTEQRPYIILKWAQ
                     TSDGYMAPLIPPKGGTSEPQTQRLFISNEYSNILVHQWRSEEAAIMVGTNTALLDNPE
                     LTTRLWPGPSPLRLVIDMNLRLPGSLKVFDCSVRTIVFNTIREDEKPNLSFIKLDKEK
                     QFIPQLMNALYRLQLQSVLVEGGAALLQTFIESGVWDEARVIVNGELSIGNGLQAPVL
                     KNEQLVKEEKISTDTISYFRNSKK"
     gene            4769..5626
                     /gene="prmC"
                     /locus_tag="PK977_08865"
     CDS             4769..5626
                     /gene="prmC"
                     /locus_tag="PK977_08865"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRF18267.1"
                     /translation="MTVQQATYFLLNQLRSIYSEGEAASMTDWVMEKITGSMKTERML
                     YKNELLQPDEVKKLEEITSRLLNHEPVQYVLNEAWFCGLKFYVDQNVLIPRPETEELV
                     EWIISNCKFPIDTLSILDIGTGSGCIPVSLKRRIRKAEVWSCDVSSPALAVAKKNAES
                     LGTEIKLLQLDFLDQNSWSQLPSFDIIVSNPPYVPEKDKNEMQPNVLNYEPHTALFVP
                     DNDPLRFYKAIAAFGKTHLNSGGTIFLEIHENLGEAVSNLLESQGYLTELKKDMQGKN
                     RMVRAYLSL"
     gene            complement(5741..6142)
                     /locus_tag="PK977_08870"
     CDS             complement(5741..6142)
                     /locus_tag="PK977_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF18268.1"
                     /translation="MGFRRKHKEESEVFTDSLNDILFILLMFFLIVATLANPNVVKVG
                     LPRGSKDTKAKQNIMVSVDKDQQYYIGTKKIDPAIFDSLLRLEIRKYRAFVDTPTVVI
                     NADTSAYYGEVFRIMKIAKKDTAKVVARIDQ"
     assembly_gap    6346..6479
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6480..6881)
                     /locus_tag="PK977_08875"
     CDS             complement(<6480..6881)
                     /locus_tag="PK977_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRF18269.1"
                     /translation="MIDLLQISDSVQKATQFAAGDTNHDGHLSYFELLKMGGCIMIPL
                     ALMMLLAVYVFAERTIAIRNAGKIDSNFMNIIRDNIVSGNITAARNFAKNNNTPVARI
                     VDKGIQRIGKPIDTIQNSMDNVGELEMYKLEK"
     assembly_gap    7059..7257
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7258..8077)
                     /locus_tag="PK977_08880"
     CDS             complement(<7258..8077)
                     /locus_tag="PK977_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRF18270.1"
                     /translation="MDFSKFLSEYWWAIVLVIIFFSGLFTINQGYIGVITMFGKYRRV
                     ARPGLNFKIPLLENVFRKVSIQNRSVELEFQAVTADQANVYFKSMLLYSVQNSEEETI
                     KKVAFKFISDKDLMQALIRTIEGNIRAYVATKKQAEILGLRREIVEFVKEHVDQSLEG
                     WGYHLQDLQINDITFDKMILDSMSKVVASNNLKAAAENEGQALLITKTKQAEADGNAI
                     KIAAEAEKEAARLRGQGVALFRREVAQGMTEAAEQMKQANLDTNVILFSMWTEAI"
     gene            complement(8109..9356)
                     /gene="pepT"
                     /locus_tag="PK977_08885"
     CDS             complement(8109..9356)
                     /gene="pepT"
                     /locus_tag="PK977_08885"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRF18271.1"
                     /translation="MFQNYKFTAAERFMRYVQIDTQSDPQSDTYPSTDKQKNLSSLLA
                     EELKAMGIVDAHMDEYGYVYATIPATTDKKVPVICFCAHVDTAPDCSGTGVKPLLHKN
                     YKGQDFILPDDKTQVLKISEYPYLKTQLGNDIITASGTTLLGADDKAGVAEIMDLANF
                     LMSHRELKHGAIKILFTPDEEVGKGTAKVDLKKLGADFGYTLDGGDAGSLEDETFSAD
                     GVKVIIHGVIAHPGYAKGKMINALKIAGEILAALPKDRLSPESTDGKRGFIHPVRVEG
                     IAEKCTIEFIIRDFETDGLKKKEDYLRTQIEERMRTYPKASFEFIVTEQYRNMKEVLD
                     LNPHVVDYAKEAINRAGLILKMESIRGGTDGSRLSFMGLPCPNLFAGEQGIHSKLEHI
                     SVQDMNKAVETMVHLVMICEERS"
     gene            9468..>9862
                     /locus_tag="PK977_08890"
     CDS             9468..>9862
                     /locus_tag="PK977_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18272.1"
                     /translation="MKKWLIGSLVGAVLLFGWQFLSWTMLKLHEAEAKYNSGQDSVLN
                     YLSSALKEDGIYMLPTVPPGTPEKEAMELGKKMDGKPWATVMYKTAYKHDMARPMIRG
                     FLVDLFLVFSLIYILTRGGTPKGMRIFAGA"
BASE COUNT         2592 a         2073 c         1870 g         2744 t
ORIGIN      
        1 ggttgtggtg ttggaagtaa aattcacttt tacattcgct tctttattca ccagcagctt
       61 gatggcattg gtacttgcat tgcccatcat atccttcatg gggcaggccg gtgttgttag
      121 cacaatcgta aatgacacat cattgccatt gatttcgata tcctgtacca tattcaacgt
      181 caccaggtct tttccaagat caggttcctg tacatttctc aacgcatcga gtatttgctc
      241 tttagtcatt ttccaagtat ttgttaaaag tgaaaaagaa aggcaaagtt aaacattgtg
      301 ccgggcaatt tgttttacga tatcggcaac ttgtctttat ttttggggca gtgaataaat
      361 ttctacttta tactggttta ctgatacttc tgactaccca aacggctaag gcccagtttg
      421 aaacttcccg tgattctgtt gtgcaattat atggtgtggt gatgaccgct gacagcctgg
      481 ttggtattcc ggccgtaagt attatggtaa agggacaaaa ccggggaact atcaccaatg
      541 cacagggtgt tttctccatc gtggtcctga aaggcgacca ggtggaattt tcgcatgtta
      601 cttataaatc caaaatggta acaattcccc gtacgctgga gggcaaccag tatagtatgg
      661 tacaactgat ggtggtggat acagtttacc tgccggccac tatcattaaa ccaaggccca
      721 ctcctgaaca atttgcccgg gactttgtga atgtaaaagt gcccgatgat gatattgaaa
      781 tagcccgccg caataccaat gctgctaacc gccgtgcttg gctgcgaacc attccgggtg
      841 atggaggtga agccacccgt cagcagttca ataaaattgc caaccgtgcg gtttaccagg
      901 gacaaacgcc tccgcaaaac atcttcaacc ctgctgcatg ggccgaattt atacaagcat
      961 ggaaacgggg agactttaaa aacaaaaagt agccacctgt cccctaaaga agagacttag
     1021 ttccaacgat cgcttctatt tctcaatttt cacaaagaag tttgcttatt aacttaacca
     1081 gctctggtat atcatggcat cgttctttcc acctcaagcg gacactttat cttgatatca
     1141 ttattcatct tatagtaaaa gcctatctca ttacaatgaa agccaaattc atatttttgc
     1201 tctgcaacta attcaatcac tcttccccaa aggagattta ccaaactccc ctttagggac
     1261 ataagaaaaa ttcaacaatc atgaatataa aagggggtat ctccgtcatc ggcgccggca
     1321 ctatgggcaa tgggattgct catgtatttg cacaaaaagg ttataaggtt actttaattg
     1381 atatttcaca ggcacagtta gataaagcca ttgcgaccat tggtaaaaat cttgaccgca
     1441 tggtggccaa agagactatc accagtgaag cgaaagaaga aaccctgaag aatatcagta
     1501 ccaatacctc tattgaagaa ggcgtgaaag atgcggcgct ggtagtagaa gccgccaccg
     1561 aaaatgtaac actgaagctg cagatcattc agcaaataga taaagctgct cctgccgatt
     1621 gcattttggc caccaatact tcttctatct ctattactaa aattgctgca gctaccaaaa
     1681 gaccggagaa agtgatcggg atgcatttta tgaacccggt accggtgatg aaannnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn atgccgatga tcaatgaagc aatatacagc ctgtacgaag gcgtggcagg
     1921 tgtggaagag attgatacgg tgatgaagct gggcatggcc cacccgatgg ggccattgca
     1981 attggcagac tttatcggcc tggatgtttg cctggcgata ttaaatgtac tgcatgatgg
     2041 ttttggcaat cccaagtatg ccccctgtcc cttgctggta aacatggtaa ctgccggtaa
     2101 actcggcgcc aaaagcggcg aagggtttta tttacacaca ccggggagta aggagttggt
     2161 ggtgagtggg agatttaaat agcaagtcta aaacagctat gaagagatgc ttgattacac
     2221 tggtgattgt tacatcaacc ataattgcat gtaaaaataa tcctcaaaag actacaatag
     2281 aacagaatgt ttcaaacgat agtacaagta ttagtattga ctgggattcc tcctatatta
     2341 taacttcatc tcaagcattt cgcactgata acggatttga attgcaaatt gacgggctta
     2401 atatagattt tcaaaatagg gagaatcgat ggtttgcctc aataaatcaa gttgccttag
     2461 ctgattgctt gttcgtttca tctcagttca gaatgctaaa ccagtcaata aaacttaaca
     2521 aagatcaagt tgacataggt gacaaattgt ggggctttat tgaattgctt acactnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt taaataaaat taagtattgc
     2701 aagtcttccc tttctactgc tgaaaatttt tgcatatcta tattttcata atagcgagat
     2761 tagcatttct ccatctccac ccctctcaac tccttcaacc ggaacaagac ttcttccagc
     2821 ctgaatttgg ggataccgat ttccagcgaa cagaataatt gtgtttcctg cttcagtacc
     2881 gggcagtnnn nnnnnnnctt tcatcacttc gttcatctgt gtatagtcga actgtatgcg
     2941 gtaattcacc agcaccggtt tttgcagcac cggtgttacc tgcagggcca ttgccgctgc
     3001 tgatttatag gcattgatga ggccgggcac tcccagcaaa gtgccgccaa aatagcggac
     3061 cacgatgatg agtacattgg tgagctgctt gctgtctatc tgtcccagta tcggccgccc
     3121 ggcagagccg gagggttcac catcatcact cacccggaag ttggtaccat ccaggcctat
     3181 gcgataggcg aaacaatgat gcgtagcctt tggatgttct tttttaatat cggccagcct
     3241 tgctttaaaa tcatttacat ctgaaacagg gaaggtaaag gcaatgaact tgctgccctt
     3301 gtcccgaaat tcggccatgg cagttgtttc aattgttttg aaatactcag gctggttgac
     3361 tgctgcggcc actttcttct cttttgatga ataaataata tacctgtatg ccgacaataa
     3421 ccgcattggg tatgataacc ggaaggttta cattcagcaa taccccatat aggatgaaca
     3481 aagccgctcc cagcatatta atagttcgca gcagccgcat attcttgcta acaaggaaac
     3541 ttataaccac caaggccgct gccatatagc caatccaaac tatccaattc atagttgtta
     3601 ctttttacta ttcctgaaat aactgatcgt atccgtactg attttttctt ccttaactaa
     3661 ttgttcattt ttcagaaccg gggcctgaag cccattacca atcgacaatt caccattcac
     3721 tattaccctt gcttcatccc aaacaccact ttcaataaag gtttggagga gcgcagcgcc
     3781 gccttctacc agcacacttt gcaattgtaa cctgtataat gcattcatca attgcgggat
     3841 aaactgcttt tccttgtcaa gtttaataaa agaaagattt ggtttttcat cttccctgat
     3901 cgtattaaaa acaattgtcc ttactgaaca atcaaaaacc tttaatgagc cgggtagtcg
     3961 caggttcata tctatcacca gcctcaatgg tgatggcccg ggccataatc ttgtggtgag
     4021 ttcgggatta tccagcaaag ccgtatttgt tccgaccata atagctgctt cctcactacg
     4081 ccattgatgt accagtatat tggaatattc gttgctaata aacaatctct gcgtttgagg
     4141 ttctgaagtc ccccctttag gggggattag gggggccata tatccatcag aagtttgggc
     4201 ccacttaaga ataatgtaag gacgctgttc cgtatgaaaa gtaaaaaacc gtttattcaa
     4261 ttccgtgcat tcctgttcca acactgctgt ctccacttct atacctgcca ccttcagttt
     4321 ttcaataccc tttccgtcta cttctttaaa agggtcacgg cacccgatga ccaccctgtg
     4381 aatattataa cgtatgatca ggtctgtaca gggtggtgtt ttgccgtaat gagcacaggg
     4441 ctccagcgac acatacagag ttgactgtga aatgaattgc ctgtcacttt ccttcactgc
     4501 attgatacag tttacttctg catgggcttt tccatattcc ttatgccagc cctcccctat
     4561 tatcctgcct tcatgcacca gcacagcgcc aaccatcggg ttgggggcca catggccggc
     4621 acccagtttg gccagttcga ggcaacgatg cataaatgat tggtcgatag tcaatgctga
     4681 ataatgaatt agaggtcaaa tataagctga ctgcgattgt cggggtatat tcgcatgcat
     4741 tgattattga caattcacta ttgacctgat gacagtacaa caggctacct attttctgct
     4801 caaccagctt cgcagcattt attccgaagg tgaagccgct tctatgactg actgggtaat
     4861 ggagaagatt accggctcca tgaaaacaga gcgaatgcta tataagaatg agctgctgca
     4921 gccggatgaa gtaaaaaaac tggaagaaat aacaagcagg ttactaaatc atgaaccagt
     4981 tcaatatgta ctgaatgaag cctggttttg cggtcttaag ttttatgtag accaaaatgt
     5041 tctcattcca aggcctgaaa cagaagaact ggtagaatgg atcatcagta attgcaaatt
     5101 tcctattgat acactcagca tactggatat aggaaccggc agcggatgta ttcccgtttc
     5161 attaaaacga aggatacgaa aagcggaagt ctggagttgc gatgtgagtt cacctgccct
     5221 ggcagttgca aaaaagaatg cagaaagctt aggcacggaa ataaaattgc tgcaacttga
     5281 tttcctggat caaaacagct ggtcacaatt accatccttt gatatcattg tcagcaatcc
     5341 gccttacgta ccggaaaagg ataaaaatga aatgcagcct aatgtattaa actacgaacc
     5401 gcatacagca ttatttgttc ctgacaatga cccgctgcgt ttttacaagg ccattgcagc
     5461 atttggaaaa acacatttga attccggcgg cactattttc ctggaaattc atgaaaacct
     5521 gggagaagct gtaagtaatt tgctagagtc acagggatac ttaacagaat taaaaaaaga
     5581 tatgcagggt aaaaacagga tggtaagggc atatttgagt ctgtagtcta tagtcaataa
     5641 tctttagtca acacatataa ctgcataagc ttggtattca ttctccacct attcaatact
     5701 atagactgtg aacttttgac tattgactat caactgccac ctactgatca atccttgcca
     5761 caacttttgc agtgtctttc ttggcgatct tcatgatccg gaacacttct ccataataag
     5821 cggaagtatc cgcattgata accacggttg gtgtatccac aaaagccctg tatttcctga
     5881 tctcaaggcg gagcaatgaa tcaaaaatag cggggtctat ctttttggtg ccgatatagt
     5941 attgctggtc tttatctacc gataccataa tattttgttt cgccttggtg tcctttgaac
     6001 ctcttggcaa accaaccttt accacattgg gattggccag cgttgccacg atgaggaaga
     6061 acatcagcag gatgaacaag atatcgttca aggagtcggt aaagacctct gattcttctt
     6121 tatgttttct tctgaaaccc attttacttg cttgaagtga taaaattgat tttacacaaa
     6181 cccattagcg ggttggctcc tgcaacacat caaggaaatc agcttctgct gcttccattt
     6241 tatttgctgt cttatcaatt tgagttttca ggtaactata ggccaggtag gcacccaaac
     6301 caatgatcaa accagtgata gaggtgatca gtttggtata gatacnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     6481 ttctccagtt tgtacatttc cagttcgccc acattatcca tactgttttg aatggtatca
     6541 atgggtttcc cgatccgctg aattccttta tctacaatcc gggcaaccgg tgtattatta
     6601 tttttggcaa aatttcttgc ggcggtgatg ttgccgctta cgatgttatc ccggataata
     6661 ttcatgaaat tactgtcgat ctttccggca ttgcggatag caatggttct ttcagcaaag
     6721 acataaactg ccagcagcat cattagggcc aggggaatca tgatacagcc tcccattttc
     6781 aataattcaa aataacttaa atgtccgtcg tgattggtat ctcctgctgc aaattgtgtg
     6841 gctttttgta ctgaatcaga aatctgtaaa aggtcgatca tagctttttt tctttaggtg
     6901 taaaagtagg gtgttgacta ctaaataata ttcagccttt atcaacagtt ctgaataaca
     6961 tcacccagaa cgagaaaaat aatgccacgt tgtactgtgg atggaagttt tagagttatt
     7021 taacaggatg gggaattagt tgactggttt atttgttgnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa
     7261 tagcttctgt ccacatactg aaaaggatca cattggtatc gaggttggcc tgcttcattt
     7321 gctctgcagc ttcagtcata ccctgtgcca cttcccttct gaataatgcc acaccctggc
     7381 cacggaggcg ggccgcttct ttctcagctt ctgcagcaat cttgatcgca ttaccatctg
     7441 cctctgcttg ttttgtttta gtgatcagta atgcctgtcc ttcattctcc gcagcagctt
     7501 tcaggttatt agatgccacc accttactca tagagtcaag gatcatctta tcgaaggtaa
     7561 tatcattgat ctgcagatcc tgcaggtggt aaccccagcc ttccaggctt tgatcaacat
     7621 gttcttttac aaactccaca atttcccttc tgagacctaa tatctcagcc tgttttttgg
     7681 ttgccacata agccctgata tttccttcaa tagtcctgat aagagcctgc atcaggtcct
     7741 tatcactaat gaatttaaat gccacttttt ttatcgtctc ttcctccgaa ttctgtactg
     7801 aatataaaag catgcttttg aaatacacat tagcctggtc agccgttact gcctgaaact
     7861 ccagttcaac agaacggttc tgtatagaaa cttttctgaa aacattttcc agcagcggta
     7921 ttttaaaatt caggcccggt cttgccaccc tgcgatattt accgaacatg gtaattacac
     7981 caatataacc ctggttgatg gtaaataaac cactgaaaaa aatgatgacc agtacaatgg
     8041 cccaccagta ttctgataaa aattttgaaa agtccataat atgtgttttt gattaatgca
     8101 atttacgatt aacttctttc ctcgcagatc ataacaaggt gtaccattgt ttctacggct
     8161 ttattcatat cctgtacact gatatgctcc agtttggaat gtataccctg ctcaccggca
     8221 aaaagattcg ggcaaggcaa acccataaag gagagacggc tgccatctgt accaccacgg
     8281 atgctttcca tcttcaatat taggccggca cggttaatag cttcctttgc ataatccaca
     8341 acatgcgggt ttagatccag cacttccttc atattgcggt attgttccgt aacaataaat
     8401 tcaaatgatg ccttgggata agttcgcatc ctttcctcta tctgtgtccg caggtaatct
     8461 tccttctttt ttagaccatc tgtttcaaaa tcccggatga tgaactcgat ggtgcatttt
     8521 tctgctattc cctccaccct taccgggtga ataaaacctc ttttgccgtc tgttgattct
     8581 ggtgaaagcc tgtccttggg caaagcagcc agtatctcac cggcaatttt taaggcattt
     8641 atcattttcc cttttgcata tcccgggtgg gcaatcaccc catggatgat cacttttacc
     8701 ccgtctgcgc tgaatgtttc atcctccagc gatccggcat cacctccatc caatgtgtag
     8761 ccaaaatcag cacctagttt tttcaggtca acttttgccg tacccttacc cacttcttca
     8821 tcaggtgtaa acaatatttt gatagccccg tgttttaact cccggtggct catcagaaaa
     8881 ttagccaaat ccatgatctc tgccacacct gctttatcat cagctcccag taatgttgtt
     8941 cccgaggcag taatgatatc attacccaac tgtgttttca ggtaaggata ctcacttatt
     9001 ttaagtacct gtgttttatc atcgggcaaa atgaaatcct gtcctttgta atttttatgc
     9061 agcaatggtt ttacacccgt accgctgcaa tcaggtgctg tgtccacatg ggcgcaaaaa
     9121 catattaccg gtactttctt atcagtagtt gccggaatgg tggcatatac ataaccatat
     9181 tcatccatgt gtgcatctac aatacccatg gccttcagct cttcagccag taaactgctg
     9241 aggttttttt gtttatcagt agaaggataa gtatcagatt gcggatcgct ttgtgtatct
     9301 atttgtacat accgcataaa tctttcggcg gctgtgaact tatagttttg aaacataact
     9361 atttattagg ggcatcgaat atacgactaa aagagggtca gtgaaaaaca cttgaccaaa
     9421 agaaggaata tcactacctt ttattcctca ccaaaaaacc aactacaatg aaaaaatggt
     9481 taatcggctc cctggtaggg gctgttctgc tctttggatg gcaatttctg tcatggacaa
     9541 tgctaaaact gcatgaagct gaagcaaaat acaattccgg tcaggatagt gtgttgaact
     9601 acctgagttc cgcattaaaa gaagatggta tttatatgtt gcctactgtt ccacccggga
     9661 ctccggagaa agaagcaatg gaactgggca aaaaaatgga tggcaagcca tgggcaaccg
     9721 tcatgtataa aacagcctat aagcatgata tggcaaggcc gatgatacgg ggtttcctgg
     9781 tcgatctttt cctagtcttt tccctgatct acattttgac ccgtggcgga actcctaagg
     9841 gtatgcgaat ttttgcagga gc
//
LOCUS       DAORLS010000098         9779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3725_length_9779_cov_2.461538, whole genome shotgun sequence.
ACCESSION   DAORLS010000098 DAORLS010000000
VERSION     DAORLS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3725_length_9779_cov_2.461538"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>831
                     /locus_tag="PK977_08895"
     CDS             <1..>831
                     /locus_tag="PK977_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF18273.1"
                     /translation="AKLGIEIKGEYPLLTDKSHIHLVEAYHPLLYLYNQKAGKSTIPV
                     SVTLDDNKRILVISGPNAGGKTVTMKTVGLLQMMVQSGLLVPVHPSSQFGIFKQLMIH
                     IGDTQSLEFELSTYSSHLLHMKYFMENANGRTLFFIDELGSGSDPSLGGAFAEVILQE
                     LAKKHAIGIVTTHYLNLKVMAGKTAGILNGAMAFDEKNLQPQYKLIIGKPGSSYTFSI
                     AERIGLDKNLINRARQLVDEDQYRLDKLLNRTEQDLRDLEKKERGLQQLLKENEKLKK
                     "
     assembly_gap    832..930
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <931..1264
                     /locus_tag="PK977_08900"
     CDS             <931..1264
                     /locus_tag="PK977_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF18274.1"
                     /translation="KDMERKLKQMLIEWRKEENKNKVIKQMEALLFKKDEKKVVSKMQ
                     KKIDAKFSEVGGEVKVGDKVKMKRNHQVGQVLELRGKRAVVKVGLLPMQVEVKDLVVV
                     KEKSTEEK"
     gene            complement(1317..1745)
                     /locus_tag="PK977_08905"
     CDS             complement(1317..1745)
                     /locus_tag="PK977_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF18275.1"
                     /translation="MNTVSLVLNRKNFSPVTITSDTTVLDALRIMAEKNIGSLVVMDG
                     EDYKGIVTERDYSRKVILKGKNSTDTRVADIMSTDLPRVSPSDKIEYCMELMSNNNIR
                     YLPVFENNRLTGIISMSDVVKETILAQKETINQLQNYIHS"
     gene            1927..2943
                     /locus_tag="PK977_08910"
     CDS             1927..2943
                     /locus_tag="PK977_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRF18276.1"
                     /translation="MSHKPATIKEIAKRLNVSISTVSRALHDHPSIGLRTKMQVQKLA
                     AEMNYEPNLTAVSFKQGKTMTLGVILPNLGEEFFSIAINGIEDVATKNKYTVLIGQSH
                     DDVEREKQIVDTMRRHRVDGIIVSLAKTTKDYNHFQQLKNYNIPVVFFDRIPDLPDAY
                     SVMCNLENSSVELVDWLVNKGHKQIGFIKGPDTIVQSAHRLQGYQQALKKHKIKPEAS
                     YIVTTDLTRKKTQDAMHKLLALKIRPTAVIAFNDYVALDAMQYARLQGIHINKDISFV
                     SYANLPLTNYLDEPPVASVEQFPYQQAEKATSVLLQLINSRNNDPDIPQKTILESKVV
                     VNKK"
     assembly_gap    2978..3060
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3159..5000
                     /locus_tag="PK977_08915"
     CDS             3159..5000
                     /locus_tag="PK977_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF18277.1"
                     /translation="MKHLKALNKYFWKYRIRLGVGVIFVFLSNYFNVLSPQVTGFVID
                     FVQRSLQLPGYKAPARPPDYDRLVEYFISWMEGIGSTGRVVALCGVTILFLALIRGFF
                     LFLMRQTIIVMSRYIEYDQKNEVYSHYQKLDTAFYKKNSTGDLMNRISEDVSRVRMFT
                     GPAIMYLANLVAIISLSVFFMLKRDTELTMYVLAPLPILAVTIYFVNNTINKKSERIQ
                     ESLSALTTNAQESYSGIRVIKSFVQEKAMLGFFTRNSEEYKKNAISLARVEAIYFPSI
                     TLLIGLSTLMTIMIGGLYYIHGGHNIGINTIVEFVMYINMLTFPVSAIGLTASMIQRA
                     AASQKRINEFLKTEPVIQNDKGAIDIIINGNISFQNVSFIYPHTGIRALSNFNLEIKK
                     GQKIAIVGRTGSGKSTIAQLLLRMYDINEGTISFEGVDIRKIDMHGLRRQISYVPQDV
                     FLFSDTVSNNISFGVDKAKQEAVLDASRQASVDREIAGFSSKFETLIGERGVTLSGGQ
                     KQRISIARALLKNPGLVIFDDCLSAVDARTEKEIISNLNQYLQDKTAIIITHRIFSLL
                     HFDKIVVLDDGKMAEEGTHEELLALNGQYASLYNRQQEQEKVLMNPQ"
     gene            5119..5535
                     /locus_tag="PK977_08920"
     CDS             5119..5535
                     /locus_tag="PK977_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRF18278.1"
                     /translation="MAYENNDKKMESIYSKRIRAGKRRTYFFDVRATRGNDYYLTITE
                     SRKRFDDNGYDRHKIFLYKEDFNKFIKALGEAVDYVKTDLMPDFDFDAFNHDENPEDT
                     IAESGHEAENTVAPDTAAAPVAEAPQQHSSEEADKW"
     gene            5968..9720
                     /locus_tag="PK977_08925"
     CDS             5968..9720
                     /locus_tag="PK977_08925"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRF18279.1"
                     /translation="MGIVETRTPASAENANRGSKAIANRTEAVCATFTGSLGAGDLTM
                     PQRLNRPGGAPSGNCAGGWVFPGTFGTGPYYYDVHTYTNNTGSTQCGTFTLSSTDVTN
                     ANIAFGIWNGSFDPNNMAANSIAAPNVSTGTPAANIVLNVTINAGQTIVIPVWSANVN
                     SSPAGTASNYTVTVEFPTCAPCVPVAAEAPIISPLPASVCAGSSVTLSVIGGNLNGAA
                     NWKWYTGSCGGTLVGTGSSITVSPAATTTYYVRGEDGCAPAPGPCATRVVTVSACTCV
                     SPESATICAGQIQRLKVSGTGAPASSTTPSGAITVSIPDASATGATHAIPVALPAGAT
                     ITSMSVNFNITHTWDADLVINLRAPNGNVLNLVNQRGGSGDNFTNTTISSASATSLAT
                     GAPPFTGTFAADAALGQGPTGNVSNVNNWAALYTGVVSGNWTLAMRDLAGGDVGTLTS
                     WSITINYTADPTATWTGPAGTIFSDPAATVPYVAGTQANTVYVQPTATSTYTANSIVG
                     GPCAGQTINIPITVNPLPTVAVSPNPGGCAPVTLTASGATVYTWSPSAGLSATTGASV
                     VATPSTNTVYTVTGVSATTGCSNTTTVSVNGTSTAAVISAPPAFISLVSEGFNSVEPT
                     LPAGWAAQNLSAPVGITGWFQGNAGVFPSQSGAPTAYIGANFNNTAGTGTISNWLFAP
                     NVTLQNGDEIEFWTRKVSPDAFPDRLELRWSANGASTNAGATATSVGDFTTLLLSVNP
                     SLITGVYPTSWTKFTATVSGLAGPTSGRVAFRYFVTNGGPSGANSDYIGIDEFSIRRP
                     LSGVCPNTVSTISVNITGGVSPYTLVYTNGTTNYTFNGYTSGTPIQVTPAVTTGYTIV
                     SVTGANGCPGVGNTGVATINVVQPPAITSQPSTTTVCAGGNATFSVGATPAIATTTFQ
                     WQVSTNGGVTWTNLTNVAPYSGVTLSTLTITGVTAGMHNNRYRVIVGGQCPPQPLTSS
                     AAVLNVNVPPAITTNPTSISRCLGTSASFTAAASVSSGNASFSYQWQVSVDGGVTFSN
                     ISGATSATYTIASVTQAMNGNRYRCVVTVAPCATTATTTAATLTVIPLPNVTISSPVT
                     QLVPGRTTTITASSTPAAAAGGWSWTLNGGSIPGTTNTQTVNIDGVGSYQATVTDVNG
                     CVNKSNVLVIGTEASDRLWIYPNPTTGQFQVRLYYGSDVAEKRAVHIYDELGQIIMTK
                     EFNLVTETPDYLQMDFDLSKQARGVYVVKVVHKYSGKIVSGLVIKQ"
BASE COUNT         2842 a         1954 c         2154 g         2647 t
ORIGIN      
        1 aggcaaaact gggaatagag ataaaggggg aatacccttt gctgacggat aaatcacata
       61 ttcacctggt agaagcatat caccccctgt tgtatttgta caaccaaaaa gcaggaaagt
      121 caacgattcc tgtttctgtt acccttgatg ataataaaag aatattagtc atcagcggac
      181 ccaatgccgg tggtaaaact gtaaccatga agacagtggg attgctgcag atgatggtgc
      241 aaagtggttt gctggtgccg gtgcatccat cctcacagtt tggtatcttc aagcagttga
      301 tgattcatat cggtgataca caaagcctgg agtttgagct aagtacttac agcagccatc
      361 ttttgcatat gaaatatttt atggaaaatg caaatggcag gacattattt tttatcgatg
      421 aactgggaag tggaagtgac ccgagtttgg gtggtgcttt tgcggaagta atattacagg
      481 aattggccaa aaaacatgcc attggaattg taaccacgca ttacctgaac cttaaagtaa
      541 tggctggaaa aactgccggt attttaaacg gagcgatggc ttttgatgaa aaaaacctgc
      601 aaccccaata taaattgatt attggcaagc ccggaagttc atataccttt tccattgcgg
      661 aaagaattgg attggataag aatcttatca acagggcaag gcagctggta gatgaagacc
      721 agtaccgcct ggacaaactg cttaaccgga cagagcagga tcttcgtgac cttgaaaaaa
      781 aggaaagagg tctgcagcaa ttgctaaaag agaatgagaa gctgaaaaag gnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caaggatatg gaacgtaagc tgaagcaaat
      961 gcttattgag tggagaaaag aagaaaataa aaacaaggta ataaagcaaa tggaggctct
     1021 tcttttcaag aaagatgaaa aaaaagtggt cagtaaaatg caaaagaaga ttgatgctaa
     1081 gtttagcgag gtcggcggtg aggtcaaagt aggggataag gtaaaaatga aacgaaatca
     1141 ccaggttggc caggtgctgg aattaagagg taaaagggct gttgtaaaag ttggattgct
     1201 gcccatgcag gtagaagtaa aagacctggt agttgtaaaa gaaaaaagta cagaagaaaa
     1261 ataaaaagcg aagccttacg gggcttcgca ggtatttaat gagtatgtaa gccgttttaa
     1321 ctgtggatgt agttttgtaa ctggttgatc gtttccttct gtgccaggat ggtttctttt
     1381 actacatcac tcatcgaaat gattccggtt agccggttat tttcaaatac aggcaggtag
     1441 cgaatattgt tattgctcat tagctccata cagtattcta ttttatcact tgggctaacc
     1501 cggggcaaat cagttgacat aatatcggca accctggtat ctgtcgagtt ctttcccttt
     1561 aaaattactt ttcgggagta gtctctttcc gttactattc ctttataatc ttcaccatcc
     1621 atcaccacca gtgagcctat atttttttct gccattattc gtaaagcatc caataccgtt
     1681 gtgtcagaag taatggtaac cggtgaaaaa ttctttctgt ttaatacaag tgaaacggta
     1741 ttcatgcagc atttctttta gtaattgaaa agtacgaaaa cttattgccc gttcaaaaac
     1801 tattttataa cagccatttt atttagtgaa catgccaatt atcctttttt agcacttaat
     1861 tgaataaatc atattccgga ttcggttgct ttttatacct tgtatcttca aagcaagatt
     1921 gcggacatgt cgcacaagcc ggccaccata aaagaaattg caaagaggtt aaatgtatcc
     1981 atttcaacag tgtcaagggc attacatgat cacccaagta ttggcctcag gacaaaaatg
     2041 caggtgcaaa aactggctgc agaaatgaac tatgagccca acctgacagc ggtgtctttt
     2101 aagcagggca aaacaatgac actgggtgtt attttaccca acttaggcga agaatttttc
     2161 tccatcgcca ttaatgggat agaggatgtt gcaacaaaaa ataaatatac agtgttgatc
     2221 gggcagtcgc atgatgatgt ggaaagagaa aaacagatcg ttgacacgat gcgccgtcac
     2281 cgggtagacg gtatcattgt ttcactggcc aagacaacaa aagactataa tcattttcag
     2341 cagctaaaga attataatat tccggtggtt ttttttgatc gtatacctga tttgccggat
     2401 gcttattccg taatgtgtaa cctggagaat agctcagtgg aactggtgga ctggcttgta
     2461 aacaaaggcc acaagcaaat tggatttatt aagggtcctg atacgatagt tcaatcagcc
     2521 caccgcttgc aaggttatca gcaggcttta aaaaagcaca agataaaacc tgaggcatcc
     2581 tatattgtaa caacggatct tacaagaaag aaaacacagg atgccatgca taaactgctg
     2641 gcattaaaaa tcagacccac agcagttatt gcttttaatg attacgtagc ccttgatgct
     2701 atgcagtatg caaggttgca gggtattcat atcaataagg atatttcttt tgtcagttat
     2761 gcaaacctgc cattaacaaa ttacctggac gaaccgcctg ttgcatctgt tgaacagttt
     2821 ccttaccagc aggcagaaaa ggcaacatct gttttactgc aattgatcaa tagcaggaat
     2881 aacgatccgg atattcctca aaaaacaatt ttagaaagta aagtggtagt gaacaagaaa
     2941 taatacttca ctctttctaa aaaaaatcaa aatcaatnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 aaaaccgttg acccaaaagg tccgggggtt cgaatccctc tctctccgct gcaaggtccc
     3121 ccaataaggg ggatttttgg tttattttca tcccatcaat gaaacatctc aaggccctta
     3181 ataagtattt ctggaaatac aggatccggc tcggtgtggg ggtgattttt gtcttccttt
     3241 ccaactattt caacgttctt tctccccagg taaccggttt tgtgatcgac tttgtgcagc
     3301 gatccttaca gttacccgga tataaggccc ctgccaggcc ccctgactat gacaggctgg
     3361 tagaatattt tatttcatgg atggaaggaa ttggttctac aggcagggta gtggcactgt
     3421 gtggcgtcac tattttattc ctggcattaa taagaggttt cttcctgttt ttaatgcgtc
     3481 agactattat tgtgatgagc cgttacattg agtatgacca gaaaaacgaa gtttatagcc
     3541 attaccagaa attggataca gcattctata aaaaaaacag tacgggtgac ctgatgaaca
     3601 ggatatcaga agatgtgagc cgggtacgaa tgtttacagg gccggccatt atgtacctgg
     3661 ccaaccttgt tgccattatt tcacttagtg ttttttttat gcttaagaga gatactgagc
     3721 ttacaatgta tgtactggca cctttgccta ttcttgcggt aactatttat tttgtcaata
     3781 atacgatcaa taaaaagagt gagagaatac aggagtcttt atcagcgctt accaccaatg
     3841 cccaggagtc ttattctggt atcagggtta taaagtcctt tgttcaggaa aaagcgatgc
     3901 tgggattttt taccaggaac agcgaagagt ataaaaagaa tgccattagc cttgccaggg
     3961 ttgaagcaat ttactttccc tcgattacac tgcttatcgg tcttagcaca ctgatgacaa
     4021 taatgatcgg aggtttgtat tatattcatg gggggcacaa tattggcatt aatactatag
     4081 tagagtttgt gatgtatata aatatgctca catttcctgt aagtgctatc gggctaacag
     4141 ccagcatgat tcaaagggct gcagcttctc aaaaaaggat aaatgaattt ctgaaaactg
     4201 aaccggtcat acaaaatgat aagggagcaa ttgatattat catcaatgga aatatcagtt
     4261 tccaaaacgt aagtttcatc tatcctcata ccggtatcag ggctttgagc aatttcaatc
     4321 tcgagattaa aaaaggccaa aaaattgcca ttgttggcag aaccggtagt ggcaaatcaa
     4381 cgatagctca gttgttgctc aggatgtatg atatcaatga aggcacaatt tcgtttgaag
     4441 gtgttgatat caggaaaata gatatgcatg ggcttagaag gcagatcagt tatgttccgc
     4501 aggatgtttt tttattcagc gatactgttt ctaataatat ctcatttggt gtggataaag
     4561 caaagcaaga agctgtgctg gatgcatcac ggcaggccag tgtagacaga gaaatagcag
     4621 gattttccag caagtttgaa accttgatcg gggagagagg tgttacgtta agcggtggac
     4681 agaaacagcg gatttcgatt gccagggcac tgcttaagaa tccagggctg gtcatcttcg
     4741 atgattgcct gagcgccgta gatgcccgca cggagaaaga gatcatttca aaccttaacc
     4801 agtacttaca ggataaaact gccatcataa ttacccaccg cattttttct cttttacact
     4861 ttgataagat agtggtgctg gatgatggta aaatggcaga agaaggtacc catgaggaac
     4921 tattggcatt gaatggccaa tatgcctcac tatataaccg gcagcaggaa caggaaaaag
     4981 tattgatgaa tcctcaatga agctgcaata acttttaggc tgatttggct gtttcaaaaa
     5041 catctctata tatttgtgaa ttcctgtttt aagacaggtt aaaccaaagc aattcattaa
     5101 ctgcaaaaat ttacaactgt ggcgtacgag aacaatgaca agaaaatgga gagcatttac
     5161 agcaaacgaa ttcgggctgg taaaagaaga acttattttt ttgatgtaag agctaccagg
     5221 ggaaatgact actacctgac cattacagaa agtcgcaaga gatttgacga caatggttat
     5281 gacaggcaca agattttttt gtacaaagaa gatttcaaca aattcattaa agcattgggc
     5341 gaagcagtgg attatgtgaa aactgattta atgcctgatt ttgattttga cgcctttaac
     5401 cacgatgaaa atccagagga tacaatagct gaaagcggac atgaagcaga aaatactgtt
     5461 gctccggata cggcagccgc tcctgtagcg gaggcgcccc agcaacacag ctcagaggag
     5521 gccgataagt ggtaaaatgt ccaaaaaata tttgaaaccc gcaaaaggcg ggttttttta
     5581 tgccagcagg catgggatat aaaaaaatca gatttatgca gcatttcaga aaaaggaatt
     5641 gtcataacca attggttatt aacattgggg ggaggaatta atttttacat tttttttaca
     5701 ggtaaataaa aaaaactatc tttcacgtca gattctggac aatacctgcc agattgatag
     5761 taatcaatca tttaaaaact acattatgca accaaaattt acgcaaaagg ctttcatgcc
     5821 aattttttcc tgtctggttg ttgttgcatt agtaagtctg tacaagcgca gacaaaaggt
     5881 aaccgaacgg caaccgggga aaaagtacaa aatccaaaag aattacttta tcaaaagcaa
     5941 caacttgagt taaagtcaaa caatgatatg ggaattgttg agactcgtac tcctgcttcc
     6001 gcagaaaatg caaatcgtgg atccaaagct atcgctaaca gaactgaagc ggtatgtgct
     6061 acttttacag gttctcttgg agctggtgat ttaactatgc ctcagcgtct taacaggccc
     6121 ggtggtgctc cgtcaggaaa ctgtgcaggt ggctgggtat tccccggaac gtttggtacc
     6181 ggtccttatt attatgatgt acatacttac actaataata caggttctac tcagtgtggt
     6241 acattcactt tgtcttctac tgacgtaaca aacgccaaca ttgcatttgg tatttggaat
     6301 ggcagcttcg accctaataa tatggcggca aatagtatag ccgcaccaaa cgtttcaaca
     6361 ggtactcctg cagcaaacat agtattgaat gtaacgatta atgctggtca aactattgtt
     6421 attccggttt ggtctgcaaa tgttaattca tcacccgcag gaactgcttc aaactatact
     6481 gtcactgtag agtttcctac ctgtgctcct tgtgtacctg tagcagcaga ggctccaata
     6541 atttctcctt tgcccgccag cgtatgtgca ggttcatcgg ttacccttag tgtaattggc
     6601 ggtaacctga atggtgctgc taactggaag tggtatactg gttcatgcgg tggtactctc
     6661 gtaggtactg gttcttccat tactgtcagc ccggcagcta caactactta ttatgtacgt
     6721 ggtgaagatg gttgtgctcc tgcgcctggt ccttgtgcaa caagagtggt tacagttagt
     6781 gcctgtacct gcgttagtcc tgaatcagct actatatgtg ccggccagat tcaaaggctg
     6841 aaagtgagtg gtacaggtgc tccggcatcc agtacaacac catcaggtgc tatcacagtt
     6901 tccatacccg atgccagtgc gacaggtgct acacatgcca ttccagtagc tcttcctgct
     6961 ggtgctacga ttacttcaat gagtgtaaac tttaatatca cccatacatg ggatgctgac
     7021 ctggtgatca acctgagggc tcccaatggt aatgtattaa acctggtgaa tcagcgtggt
     7081 ggcagtggtg acaactttac caataccact atcagttctg cttcagctac atccttagca
     7141 acaggagctc caccgttcac aggtaccttt gctgctgatg ctgctttggg tcaaggccca
     7201 accggtaatg tgtctaacgt aaataactgg gctgcacttt acacaggtgt agttagtggt
     7261 aactggacac tggctatgag ggatcttgct ggcggagatg ttggtactct tacatcatgg
     7321 tcaatcacta ttaattatac agctgatcct actgctacct ggaccggccc tgcaggtact
     7381 attttcagtg atcctgctgc aacagttccc tatgttgccg gtacacaggc caatacagtt
     7441 tatgtacagc caactgcaac ctcaacgtat acagctaatt caattgtagg tggtccttgt
     7501 gctggtcaaa cgatcaatat tccaattacg gtgaacccgc tgcctacagt ggctgtttca
     7561 cctaatccag gtggttgtgc tcctgtaaca cttactgcat ctggcgccac tgtttatacc
     7621 tggtctccaa gtgcaggctt aagcgcaaca acaggtgcca gtgtagtggc aacgccttca
     7681 acaaataccg tttatactgt aactggagta tcagctacta ccggttgttc taatacaacc
     7741 acggtatctg taaatggtac atccaccgct gcggtaattt cagcacctcc tgcctttatt
     7801 agcctggtga gtgaaggatt taatagtgtt gaaccaacgc tacctgctgg ctgggctgca
     7861 caaaacctta gtgcaccggt tggaattaca ggatggttcc agggcaatgc cggagtattc
     7921 ccttcacaga gcggtgctcc aacagcttac ataggagcca acttcaacaa tacagcaggt
     7981 actggtacaa tcagtaactg gctgtttgcc ccgaatgtaa cattgcagaa tggtgatgaa
     8041 atcgagttct ggacaagaaa agtatctccg gatgctttcc ctgacagact tgagctgcgc
     8101 tggagtgcaa atggtgcaag taccaatgca ggtgcaactg ctaccagtgt aggagatttc
     8161 actactttgt tattatcagt gaatccgagt cttattaccg gagtttaccc aacaagctgg
     8221 actaagttca cagctactgt atcaggtctt gccggtccta catctggacg agttgccttc
     8281 cgttattttg taaccaatgg cggaccttca ggtgctaatt cagactacat cggaatagat
     8341 gagttttcaa tacgtcgtcc gttatccggc gtatgtccca atacagtatc tacaatttca
     8401 gttaatatta cgggtggagt atctccatat accttagtat atacaaacgg aacgaccaac
     8461 tataccttta atggttacac aagtggtaca ccaatccagg ttacccctgc tgtaacaaca
     8521 ggctatacga ttgtttccgt aacaggtgcc aatggatgtc ctggtgtagg taatacaggt
     8581 gttgctacta ttaatgtggt tcagcctcct gctatcacca gccagccgag tactacaaca
     8641 gtatgtgctg gtggcaatgc tacctttagt gtaggagcca caccggcgat agcgactaca
     8701 acgttccagt ggcaggtaag taccaatggt ggtgttacct ggaccaacct gaccaatgtg
     8761 gctccttatt caggagtaac attatctact cttactatta ctggtgttac agccggtatg
     8821 cataacaaca ggtatcgtgt aattgtggga ggtcagtgtc ctcctcagcc gctgaccagc
     8881 agcgctgctg ttttgaatgt aaatgttcct ccggctatta caaccaaccc gaccagcatc
     8941 agcaggtgtt tgggtacatc agccagcttc actgctgcgg ccagcgtatc aagcggtaac
     9001 gcttcattca gctaccagtg gcaggtgagt gttgacggtg gtgttacatt ctccaacatc
     9061 agtggtgcta cttcagctac ctataccatt gccagtgtaa cacaggccat gaatggtaac
     9121 aggtaccgtt gtgttgtaac cgtagcacct tgtgctacaa cagcgactac cactgcagca
     9181 acgctgacag tgataccgct gcctaatgtt acgatttcat caccggttac tcagctggtt
     9241 ccgggtcgta ctaccactat cacagcatcc agtactccgg ctgcagcagc aggcggatgg
     9301 tcctggacat taaatggtgg ttctataccg ggtacaacta acacccagac tgttaatata
     9361 gacggtgttg gttcatacca ggctacagta acagatgtaa acggatgtgt gaacaagtca
     9421 aatgttctgg taattggtac ggaagcatca gatcgtctgt ggatctatcc taacccgact
     9481 accggtcagt tccaggtaag attgtactat ggaagcgatg tagcggagaa gagagccgta
     9541 catatctacg atgaactggg ccagatcatc atgaccaagg agttcaacct ggtaaccgag
     9601 acaccggatt acctgcagat ggacttcgat ctgagcaagc aggcccgtgg tgtgtatgtg
     9661 gtgaaagtgg tacacaagta ttcaggtaag attgtatcag gacttgtgat caagcaataa
     9721 ccgaaataca acgaataaca gatatcgcaa aacgcccctc tcaaagaggg gcgtttttt
//
LOCUS       DAORLS010000099         9747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3740_length_9747_cov_1.788382, whole genome shotgun sequence.
ACCESSION   DAORLS010000099 DAORLS010000000
VERSION     DAORLS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_9747_cov_1.788382"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK977_08930"
     CDS             complement(<1..192)
                     /locus_tag="PK977_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRF18280.1"
                     /translation="MGEYSISEAMQQFLNSSRIKGGIQALQIEDVWEEIMGKTIARYT
                     DKLQIIGDKLIITTSVAPLK"
     assembly_gap    227..254
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<255..>519)
                     /locus_tag="PK977_08935"
     CDS             complement(<255..>519)
                     /locus_tag="PK977_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRF18281.1"
                     /translation="DDLDMHLGSQPFKNVASQGQRKSLLFSLKLAEMEVLKKEKGFAP
                     LLLLDDVFEKLDEERVNNLLNKVCVENDGQVFITDTNGERLSSH"
     assembly_gap    520..577
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<578..1297)
                     /gene="recF"
                     /locus_tag="PK977_08940"
     CDS             complement(<578..1297)
                     /gene="recF"
                     /locus_tag="PK977_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626633.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRF18282.1"
                     /translation="MLQLQQITLYQFKNYRSSSFDFGERIVGICGKNGVGKTNLLDAI
                     HYLCFTKSYFTRHDNQNVQHGSDGFRLEGRFALYDKPEKTVCILRETGKKEFSVNDAP
                     YDKFSKHIGRYPCVVIAPDDAELITGDSKERRIFIDALLSQVDEEYLRQLITYKKVLD
                     QRNSLLKSFAETGKTDLALLDVLDEQLAKAGVYIFEKRKFFMVSFLPVVKRLYQDISR
                     QPEDVNLFYESELLQATIAELL"
     assembly_gap    1358..1391
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1469..2464
                     /gene="pdhA"
                     /locus_tag="PK977_08945"
     CDS             1469..2464
                     /gene="pdhA"
                     /locus_tag="PK977_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRF18283.1"
                     /translation="MATKFSKETYLYWYELMQLIRQFELMAEEKYKMDGKIRGFFHAY
                     VGQEAIAAGCMTATRPEDLFITAYRDHGLAIAKGVSVNSCMAELYGKATGCAKGKGGS
                     MHFFGKEANFYGGHGIVGAQIGTGAGLAFAEQYRGTDNVVLTYFGDGAARQGMLHETF
                     NLAMLWKLPVIFICENNNYAMGTSVERTSNVIDIYKLADAYEMPADTIDGMSAEAVHE
                     GVSRAVKRAREKGGPSLLEIKTYRYKGHSISDPQKYRTKEEVDEYKSKDPIQQVLRTI
                     LENKFATQEEIDVINKRVDDIVVESVKFAEESPWPDDSEVLKDVYVDQNYPFIVN"
     gene            2499..3191
                     /locus_tag="PK977_08950"
     CDS             2499..3191
                     /locus_tag="PK977_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18284.1"
                     /translation="MSEKKVVQDQDSSEVVIAKAKDFWTRYGKVLTIASIVVILGAGG
                     WYAYQNFFKKPKETKASEAMFKAEEYFRLDSVNLALNGDGQHLGFLKVIDKHSGTDAA
                     NLANYYAGVCYIKLNENEKAVKHLKKFSSNSKPVQARAYKLIADAYGDMGKNKEAFDY
                     YKKAGYHFPEDEFNSAESLFMAAYLAQRVLNDTKSAIELYKEIKEKYPTSDQGRDADN
                     YLAQMGVYSTEK"
     gene            3195..3692
                     /gene="ribH"
                     /locus_tag="PK977_08955"
     CDS             3195..3692
                     /gene="ribH"
                     /locus_tag="PK977_08955"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRF18285.1"
                     /translation="MAEIANSKLLSNSTGILKKDACIVLVKTEWNAAIVDQLERGAIA
                     ELEKQGIKKTIVLTVPGAVEIPFSIKAFWENSGKKARPDAFIALGCVIKGDTPHFDYV
                     CKAVTDGVLQLNLQLPVPVIFGVLTVNNEEQAMERIGGKHGHKGEEAAITAVKMISLV
                     RSFKK"
     gene            3697..4416
                     /locus_tag="PK977_08960"
     CDS             3697..4416
                     /locus_tag="PK977_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRF18286.1"
                     /translation="MNVQLFIPCFIDQLNPDTAFNMIKVLEKAGCTVSYNESQTCCGQ
                     PAFNAGYWNEAKSVCSKFIKDFSGTDYIVGPSASCVGFVRNYYAKLFDNSSVHNEVKE
                     LGKRIFEFSEFLVNVLNIENVGASLNGKATYHDSCAGLRECKIKKEPRKLLANVKGLE
                     LVEMNDVETCCGFGGTFAVKFEPISIGMGEQKVENALATGAEYLISTDYSCLMHLQGY
                     IGQKGYNLKTMHLADVLATGW"
     gene            complement(4477..4680)
                     /locus_tag="PK977_08965"
     CDS             complement(4477..4680)
                     /locus_tag="PK977_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF18287.1"
                     /translation="MELDEWIKKGSGTAELTTVAGGKLWVMKKDGKYMLKDENGGMAT
                     VTIGNVYQSNGVIHVIDHVVLPK"
     gene            complement(4698..5183)
                     /locus_tag="PK977_08970"
     CDS             complement(4698..5183)
                     /locus_tag="PK977_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF18288.1"
                     /translation="MPYVRSLNLVFKNTGLWNNKVYTILKPEIAVYRISFVSIFLNYK
                     AEEMKSIKSICLMLVASVFFHASFAQNMMNEKTVEVGGAPMYPSKNIVENAVNSKDHT
                     TLVAAVKAAGLVETLQGTGPFTVFAPTNAAFDMLPKGTVENLVKPENKAKLTSILTTM
                     S"
     gene            5177..5587
                     /locus_tag="PK977_08975"
     CDS             5177..5587
                     /locus_tag="PK977_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRF18289.1"
                     /translation="MAYWLIKSEPFKYSWEQFEKDKQTFWDGVRNYAARNNLRAMKKG
                     DLAFFYHSNEGTEIVGIAKVVKEAYQDPTTNETAWVAVDFKPYKKLKKPVSLAQIKAD
                     KRLANMALVRLGRLSVQPVTDQEWEVVMEMADEQ"
     assembly_gap    5694..5801
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5802..6119
                     /locus_tag="PK977_08980"
     CDS             <5802..6119
                     /locus_tag="PK977_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18290.1"
                     /translation="GTEPVIEIKKDLIIGKWKTEAQPGNDSTFKDYQYEFSKKGNIIR
                     SLNDSTTADSVFYTWDKENNLEWKKNAADTTGVLYKVVQLTADSLQLLSKDSSVILFT
                     RLK"
     gene            6170..>6269
                     /locus_tag="PK977_08985"
     CDS             6170..>6269
                     /locus_tag="PK977_08985"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRF18291.1"
                     /translation="MSKKKLVILTGAGISAESGLKTFRDSDGLWEGY"
     assembly_gap    6270..6291
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6292..6875
                     /locus_tag="PK977_08990"
     CDS             <6292..6875
                     /locus_tag="PK977_08990"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRF18292.1"
                     /translation="VATPRAWRMNPQLVLDFYNMRRKNVADAQPNDAHYGMAELETEF
                     DVTVITQNIDDLHERAGSTKILHLHGEIFKMRSEINESLIYEIRDDIKLGDVAEDGAQ
                     LRPHIVWFEEPVPMIEKAIPVVRDAEIFVVAGTSLVVYPAAGLVNYTQPGIPVYVLDK
                     RIPYVSSNGNLVTIEKPATEGIKELISLLKNNF"
     gene            6875..7339
                     /locus_tag="PK977_08995"
     CDS             6875..7339
                     /locus_tag="PK977_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18293.1"
                     /translation="MDDLSFGKLLIPILVVFILLVIPYIFFLLAQQSILKAIQPQNRL
                     MQPGEVWFQLIPLFGWIWQFAVVSRIADSICNEYKSQQEISFLGMGDGEIQEDIKNRA
                     TYTTGYWYCVLVCTTIIPILGILTGLGALVLWITYWQQLNKHRQKILDSNLA"
     gene            complement(7336..>7530)
                     /locus_tag="PK977_09000"
     CDS             complement(7336..>7530)
                     /locus_tag="PK977_09000"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRF18294.1"
                     /translation="VKGENSLHTAMAKTVGLPLGIAAKLILNGKIKMKGLHIPTAKAI
                     YEPVLAELEANDIYFVEKRS"
     assembly_gap    7531..7577
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7578..>7934)
                     /locus_tag="PK977_09005"
     CDS             complement(<7578..>7934)
                     /locus_tag="PK977_09005"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18295.1"
                     /translation="GLLENLMKLVDAETEAENEGEKIPSSFLAADDKGNLQEVEIDEV
                     KNRAAAYLAHKMHEANLTLKQLFFLGLDDNETLVNKGLCSPADILQFAVEKKLALRPY
                     DKDMIVMMHEIEYNIT"
     assembly_gap    7935..8021
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8022..>8312)
                     /locus_tag="PK977_09010"
     CDS             complement(<8022..>8312)
                     /locus_tag="PK977_09010"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRF18296.1"
                     /translation="AGKAGAHFREAGQEVKIPYEQLFTGERMVEIADVGYLSWYANRD
                     SLSYTSLYDLENTSTFIRTTLRHPDFMYGWKNIIELKLTEETPQYETDGKSLQ"
     assembly_gap    8313..8333
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8334..8848)
                     /locus_tag="PK977_09015"
     CDS             complement(<8334..8848)
                     /locus_tag="PK977_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRF18297.1"
                     /translation="MKTILLFGAGKSATVLIDFLTKNAAEENWKIIVADADLQLAESK
                     IGGSPYASAVAFDIKSQAEREKYIRKADIVISLLPPFLHILVAKDCIKHHKNLLTASY
                     LDEDIRSLQPEIEKNNLFFLYEMGLDPGIDHMSAMKLIDEIRQIGGHITSFISHCGGL
                     VAPESDDNPWH"
     gene            9099..>9747
                     /locus_tag="PK977_09020"
     CDS             9099..>9747
                     /locus_tag="PK977_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRF18298.1"
                     /translation="MEENIEPLETNDSNRIIPVNIEEQMKTAYIDYSMSVIVGRALPD
                     VRDGLKPVHRRVLFAMNELGIHYNKPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQ
                     EWNMRYTMIDGQGNFGNQDGDGPAAMRYTEARLERLAEHMLDDIEKDTVDFQLNFDDS
                     EKEPSVLPTRVPQLLINGSSGIAVGMATNMMPHNLSEVIDGCIAYIDNREITIEEL"
BASE COUNT         2837 a         1804 c         2016 g         2685 t
ORIGIN      
        1 cttcagggga gctactgatg tagtgatgat cagtttatct ccaattattt gaagcttatc
       61 ggtataccta gcaatggttt tgcccattat ttcttcccaa acatcttcta tttgcaaggc
      121 ctgtatgccg cctttgatcc ggctgctgtt cagaaattgc tgcatagctt cactgatgga
      181 atattcaccc ataattcaaa gttacaaact gatcaaatgg tattttnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnggtgag aagaaagtct ttctccgttg gtgtctgtaa taaatacctg
      301 gccatcattt tctacgcaaa ctttattcag cagattgttt accctctcct catccagttt
      361 ttcaaaaaca tcatccagta agaggagggg agcaaatcct ttttcttttt ttaagacttc
      421 catttcggcc agtttcaggg agaacaaaag gcttttacgc tgtccttgcg aagccacatt
      481 cttaaatggc tgacttccca gatgcatatc caaatcatcn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa tagttctgca attgtcgcct
      601 gcaacaattc gctttcataa aaaaggttta catcttctgg ttgacgggaa atatcctggt
      661 acagacgttt cacaacaggt aagaatgaaa ccatgaaaaa tttacgtttt tcaaaaatat
      721 atactccggc ttttgctaac tgttcgtcta acacatccag taaagcaagg tctgtttttc
      781 cagtttcagc aaagcttttt aacagagagt tgcgctggtc aagcactttt ttataagtga
      841 taagttgcct caggtattct tcatccacct ggctcaacaa ggcatcaata aatattcttc
      901 tttctttgct gtcgcctgta atcagttctg catcatccgg cgcaattact acacatggat
      961 aacggccgat atgctttgaa aacttatcat acggggcatc attgacagaa aattcttttt
     1021 taccggtttc tctgagtatg cataccgttt tttctggttt gtcatataaa gcaaaccggc
     1081 cttccagcct gaatccgtcg ctgccatgct gtacattttg gttgtcatga cgggtgaaat
     1141 agctttttgt aaaacagagg tagtgaatag cgtccagtaa gttggttttt ccaacaccgt
     1201 ttttaccaca aattccgaca atcctttcgc cgaaatcgaa tgagctactg cggtaatttt
     1261 taaactggta aagggttatt tgctgtagct gtagcattgc tgcaagataa tggggatacc
     1321 ccaacacaca agcgacaacc ccagagagga tataaacnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn ntcaaatttc tgtaatccgg ccccgaatat gataaattga catctttccc
     1441 ttatttttgc ggctcaaatt tttagccggt ggctaccaaa ttttctaaag aaacctacct
     1501 gtactggtac gaattaatgc aattgatccg tcagtttgaa ctgatggccg aagaaaagta
     1561 caaaatggac ggcaagatcc gtggcttctt ccatgcttat gttggacagg aagccattgc
     1621 agccggttgc atgacggcca cccgcccgga agatcttttt attactgctt accgtgatca
     1681 tgggctggct attgcaaaag gcgtttccgt taatagctgc atggctgaat tgtatggcaa
     1741 agccacaggt tgtgccaagg gaaagggtgg aagtatgcac ttttttggta aggaagccaa
     1801 cttttatgga ggtcatggta tagtgggtgc ccagattggt accggagccg gtttagcttt
     1861 tgctgagcaa tataggggta cagataatgt ggtacttact tactttggcg acggtgctgc
     1921 aagacaaggt atgttgcatg aaacctttaa cctggccatg ctctggaaac tacccgtgat
     1981 ttttatctgt gagaataaca attacgccat gggtacttct gttgaaagga cctctaacgt
     2041 tattgatatt tataaattgg cagatgccta cgaaatgcca gcagacacga tcgatggtat
     2101 gagtgcggaa gccgttcatg aaggagtaag cagggctgtg aaaagagcta gagaaaaggg
     2161 cggaccttct ttattggaaa ttaaaacata ccgctataaa ggccactcaa tcagtgatcc
     2221 gcagaagtac agaactaagg aggaagtgga tgagtacaag agcaaagacc ctattcagca
     2281 ggtattaaga accatcctgg aaaacaaatt tgccacccag gaagaaattg atgtgatcaa
     2341 taaaagagta gatgatattg tagtggaatc tgtaaaattt gctgaggaaa gcccatggcc
     2401 ggatgacagc gaagtactta aagacgttta tgtggatcaa aactaccctt tcattgtaaa
     2461 ttaattttta aatcattttg taaaatagaa tttgaattat gtcagaaaag aaagttgtgc
     2521 aggaccagga cagtagcgaa gtggtgattg ccaaagccaa agatttctgg acccgttatg
     2581 gtaaagtact tacaatcgcc agtatagtag ttattctggg tgctggtggg tggtatgcgt
     2641 accagaattt ctttaaaaaa ccgaaagaaa ctaaagcgtc agaagcaatg ttcaaagcgg
     2701 aagagtattt caggctggac tctgttaacc tggcactgaa cggtgatggc cagcatttag
     2761 gtttcctgaa agttattgat aagcacagtg gcactgatgc ggctaatctt gctaattact
     2821 atgctggtgt ttgttacatt aaactgaatg aaaatgaaaa ggcagttaag cacctgaaaa
     2881 agtttagcag caattcaaag ccagttcagg caagagccta taaacttatt gctgatgcat
     2941 atggtgatat gggcaagaat aaagaagctt ttgattatta taaaaaggct ggctatcatt
     3001 tccccgagga tgagttcaat tcagccgaga gcctattcat ggccgcttat ttagcacaaa
     3061 gagttttgaa tgataccaaa agcgccattg aactttacaa agagataaaa gaaaaatacc
     3121 ctacttctga tcagggccgt gatgcagata actacctggc ccagatggga gtttacagca
     3181 ctgaaaagta atctatggct gaaatagcta acagtaaatt attaagcaat agcacaggca
     3241 ttctaaaaaa ggatgcctgt attgttttgg tgaaaacgga atggaatgct gctattgttg
     3301 atcagttgga acggggcgcc attgctgagc tcgaaaagca aggcataaaa aagacaatcg
     3361 ttctgactgt tccgggtgca gtggaaattc ctttctcaat aaaagcattt tgggaaaaca
     3421 gtggtaaaaa ggcaagacct gatgctttta ttgcattggg ttgtgtgatc aaaggtgata
     3481 ctccccattt cgattatgta tgtaaagcag taacagatgg agtgctacaa ttaaacctac
     3541 aattgccagt gcctgttatt tttggtgttc tgacagtaaa caatgaagaa caggccatgg
     3601 aacgtatagg tggtaaacac ggacataaag gggaggaagc tgctattact gctgtaaaaa
     3661 tgatttcttt agtaaggtct ttcaaaaaat aacccattga acgttcagct tttcatacca
     3721 tgttttattg accagctaaa cccggatacg gcatttaata tgatcaaagt gctggaaaaa
     3781 gctggctgta ctgtcagcta taatgaatca caaacctgtt gtggccaacc agcatttaat
     3841 gccggatatt ggaatgaagc taaatctgta tgctctaaat tcatcaaaga tttcagtgga
     3901 accgattaca ttgttggacc cagtgccagt tgtgtgggtt ttgtaagaaa ttattacgcc
     3961 aaattattcg ataattcatc tgtccataat gaggtgaaag agttgggcaa acgcattttt
     4021 gaattttcag agttcttagt caatgtattg aatatagaaa atgttggcgc atcattgaat
     4081 ggcaaagcga cttatcatga tagttgcgcc ggactcagag aatgtaagat taagaaggaa
     4141 ccaagaaagt tattagctaa tgttaaggga ttagaacttg ttgaaatgaa tgatgtagaa
     4201 acctgctgtg gctttggcgg aacctttgca gtgaaatttg aacccatttc aattggaatg
     4261 ggggagcaaa aagtagagaa tgcattggct accggtgcag aataccttat ttcaactgat
     4321 tattcctgcc ttatgcattt gcagggctat attggtcaaa agggatataa tctaaaaacc
     4381 atgcacctgg cagatgtatt ggccaccggc tggtaaaata aagctggtat gtaaacagcc
     4441 agcttcattt cattacaaaa acaagtaacc cgattgttat ttcggcagta ctacatgatc
     4501 aatcacatgt attacgccat tgctctggta tacattaccg atagttactg tggccatacc
     4561 gccgttctcg tctttcagca tatattttcc gtcttttttc attacccaaa gtttaccacc
     4621 tgcaactgtt gttaattcag cagtcccgct tcctttttta atccactcat ccaactccat
     4681 ggtgccaagg cgtcctgcta cgacatggta gttagaatac tggtaagttt ggccttgttt
     4741 tccggtttaa caaggttctc aaccgttccc tttggcagca tgtcaaatgc agcattagta
     4801 ggagcaaaaa cagtaaatgg cccggtaccc tgtaatgttt ctaccaatcc ggcagctttt
     4861 acagcagcca ccagggtagt atgatctttt gaattaacgg cattctcaac gatgtttttc
     4921 gatggataca tcggtgcacc gcctacctct actgtttttt cgttcatcat gttctgtgca
     4981 aatgatgcat ggaagaacac agaagcaacc agcatcaggc aaatggactt aattgatttc
     5041 atttcttcag ctttatagtt taaaaaaatt gatacgaaag agatacggta aactgcgatt
     5101 tccggtttta aaatggtata aactttatta ttccacaacc ctgtattttt gaaaactaaa
     5161 ttcaaacttc ttacgtatgg catactggct tattaagtct gaaccgttta aatattcctg
     5221 ggaacaattc gaaaaagata aacagacctt ctgggacggt gtaagaaact atgcagcccg
     5281 aaataaccta agagctatga aaaaagggga ccttgctttt ttttatcata gtaatgaagg
     5341 gacagagatc gttggtattg ccaaagtggt taaagaagcc taccaggatc caactaccaa
     5401 tgaaacagcc tgggtagccg ttgactttaa accgtataaa aaattaaaga aacccgttag
     5461 tcttgcacaa ataaaagcag ataaacgttt ggccaatatg gctcttgtta ggttgggccg
     5521 cttatctgtg caaccggtta ctgatcaaga atgggaagtg gtaatggaaa tggccgatga
     5581 gcaataataa acttctccaa caaatggatt acttgtcaca tcctccgaaa gatttcgttt
     5641 aacatttcat ggaggttacg tttttgggtt agctggaata aacagaagat tctnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcactgaa cctgtcattg
     5821 aaatcaaaaa ggatcttatt attggcaaat ggaagacgga agcacaaccg ggtaacgatt
     5881 ccacttttaa agattaccag tatgaatttt caaaaaaagg gaatattatc cggtcattaa
     5941 atgattctac aactgcggac agcgtctttt atacttggga taaggaaaat aatcttgaat
     6001 ggaaaaagaa cgcagcagat acaacaggtg tgttgtacaa agtcgtacaa ctgactgctg
     6061 acagcttaca attattgagc aaagacagtt ctgttattct gtttacccgg ttgaaataat
     6121 cagccctgat actatgccac aagtttaagt ggcatagtat ctttgataaa tgtcaaaaaa
     6181 gaaattagta atacttaccg gtgccggtat cagcgctgaa agcggattaa aaacatttcg
     6241 tgacagtgat ggactatggg aaggatatgn nnnnnnnnnn nnnnnnnnnn naagttgcta
     6301 caccaagagc ctggagaatg aacccgcaac tggttctgga tttctacaat atgcgccgaa
     6361 aaaatgtagc tgatgcacaa ccaaatgatg cgcattatgg catggcagaa ctggaaacag
     6421 aatttgatgt caccgttatt acccagaata ttgatgatct gcatgaaaga gccggttcaa
     6481 ccaaaattct tcatctgcac ggagaaatct ttaaaatgcg gagtgaaata aatgaatcac
     6541 tgatttatga aataagagat gatataaaac tgggtgatgt agcagaggat ggggcacagc
     6601 tccggccgca tatcgtttgg tttgaggaac cggtacccat gatcgaaaaa gccattcctg
     6661 tggttcggga tgctgaaata tttgttgtag cagggacatc tctggtagtt tacccggctg
     6721 caggtttggt aaattatacc cagccgggaa ttcctgttta tgtattagat aaaaggatac
     6781 catacgtttc ttcaaatggg aacctggtaa cgatagagaa accggccaca gaagggataa
     6841 aagaattaat ttcccttctt aaaaacaatt tctgatggat gatttaagtt ttgggaaact
     6901 attgattcca atactggttg tctttatcct cttggttatt ccttacatat tttttctgct
     6961 ggcgcagcaa agtatattga aagccataca gccacaaaac aggctgatgc agcccggtga
     7021 agtgtggttt cagcttatac cattatttgg ctggatatgg cagtttgcag tggtgagccg
     7081 aattgcagat tctatttgta atgaatacaa atcacagcag gaaatttcat ttctcggaat
     7141 gggtgatggc gaaatacaag aagacattaa gaaccgtgca acgtatacga ctggttactg
     7201 gtactgtgta ttggtatgta caacaataat cccaatcctt ggaatactca caggattagg
     7261 ggccctggta ctttggataa cctattggca acagctcaac aaacaccggc aaaaaatact
     7321 ggattccaac ctggcttagc ttcttttttc gacaaagtaa atatcattag cttccagctc
     7381 agccaaaacg ggctcgtaga ttgctttggc tgtcgggata tgtaaaccct tcattttgat
     7441 tttaccattg agtatcagct ttgcagctat ccctaatggt aatccaactg ttttagccat
     7501 ggctgtatgc aaactattct cacccttcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnngag taatattata ctcaatttca tgcatcatta cgatcatgtc
     7621 tttatcgtag gggcgaagag ccaatttttt ctcgactgca aactgaagga tgtcggctgg
     7681 actacaaagt cctttgttca ccaatgtttc gttatcatca agtccgagga agaataattg
     7741 ttttagagtc aaattggctt catgcatttt atgggccaga taggctgcag ccctgttttt
     7801 tacttcatca atttctactt cctgcaggtt gcctttatca tctgccgcca aaaatgaaga
     7861 aggaattttt tcaccctcgt tttctgcttc ggtttccgca tccacaagct tcatcaggtt
     7921 ttccagcagc ccttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgtaacga cttaccgtcc
     8041 gtttcgtatt gtggtgtttc ctctgtaagc tttagctcaa tgatattttt ccagccatac
     8101 ataaaatccg gatggcgaag agtagttctg ataaaggtcg aagtgttttc taagtcatat
     8161 agtgaagtat agctcagcga atccctgttg gcgtaccagc tcaggtaacc aacatcggct
     8221 atttccacca ttctttcgcc agtaaaaagc tgttcgtagg gaatcttcac ttcttgtcct
     8281 gcttcacgaa aatgggcacc ggctttacct gcnnnnnnnn nnnnnnnnnn nnntaatgcc
     8341 agggattatc gtcactttca ggagcaacta atccgccaca atgagaaata aacgaggtaa
     8401 tatgcccgcc tatttgccgg atttcatcaa taagtttcat ggcactcata tgatcaatac
     8461 ccggatcaag gcccatttcg tatagaaaaa acaggttatt cttttcaatc tctggctgaa
     8521 gcgaacggat atcttcatca aggtatgatg cagtcagtaa atttttatgg tgctttatac
     8581 aatcctttgc taccaggatg tgcaggaaag gaggaagcag ggaaataaca atatcagcct
     8641 tacggatgta tttttctctt tcagcctggc ttttaatgtc aaaagcaacg gcactggcgt
     8701 aaggcgatcc tccaatcttg gactcggcca attgcaaatc agcatccgcc acaatgattt
     8761 tccagttttc ttcagctgca tttttagtca ggaaatctat caaaactgta gccgatttgc
     8821 cagcgccgaa caataaaatc gtcttcataa aaattccggt gttgaaaaag cgaaaattag
     8881 aactaaaatc ccagaaaatg actgaaaaat cacaggtgaa actcctaaag aatcaacttt
     8941 tattttaatt cacaagggtg gaaaaaatag gttaaaaaac actggttttt atcaggtttt
     9001 gagggctgaa aaaagtagat ttgcagacct taaaaatagg cccataacag ggttattttg
     9061 aggacgaaaa aaaacaaaac tgaataggaa acctagcgat ggaagagaat attgagcctt
     9121 tagaaacgaa cgacagcaac cggatcattc ccgttaatat tgaagaacaa atgaagactg
     9181 cctacattga ctattcaatg tcggtaattg tgggccgtgc ccttccggat gttcgggatg
     9241 gtttaaaacc agttcaccgc cgtgtgttat tcgccatgaa tgagctggga attcattaca
     9301 acaaacctta taaaaaatcg gcccgtatag tgggggaggt attgggtaaa taccatcctc
     9361 atggtgatag ctccgtttac gatgccatgg taaggatggc acaggaatgg aatatgcggt
     9421 ataccatgat tgacggccag ggtaactttg gcaaccagga tggagatggg cccgcggcca
     9481 tgcgatatac agaggctaga ttggaaaggc ttgcagagca catgctggat gatattgaaa
     9541 aagacacagt tgatttccag ctaaattttg atgactcgga aaaagagcct tcagttcttc
     9601 ctacccgggt tccgcaatta ctgataaatg gatcaagtgg cattgcggta gggatggcaa
     9661 ccaacatgat gccgcacaac cttagcgagg tgattgatgg ctgtattgcc tatatcgata
     9721 accgggagat taccattgag gaactga
//
LOCUS       DAORLS010000100         9721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3754_length_9721_cov_2.232154, whole genome shotgun sequence.
ACCESSION   DAORLS010000100 DAORLS010000000
VERSION     DAORLS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3754_length_9721_cov_2.232154"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..>1136
                     /locus_tag="PK977_09025"
     CDS             38..>1136
                     /locus_tag="PK977_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18299.1"
                     /translation="METTAQVNTVEFGKNRVQYQKFKWKYYQTDNFNTYFSQDGLGLG
                     KYVAQVAELELPSIEEFVEYGLQRRINIVVYNNYDEMQQSNIGLGIDWQNTGGVTKLV
                     NNKMIVYFDGNHDNLRRQIRQGIAKNLVDNLLFGDDLGEFAANQALLDLPKWLTDGYI
                     AYAAENWSTTLDDDLRGVMLAGDYKNFYQFAFAKPMLAGHAFWKYIADKYGKNKTTYF
                     LYLARIYRNLNNASNKIAKKKFKKILEDFMTDVQQQYFKDIRGRRNTPRGQLSVSESI
                     GKKDFIRFNANPLPRSFTYAVVEFKQGRYQVVLMENFVNRRVLLKLGVRTRENEKHPN
                     YPLLAWDGKGTRLAVLYNDEGKTKFFVYDVVN"
     assembly_gap    1137..1151
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1152..3400
                     /locus_tag="PK977_09030"
     CDS             <1152..3400
                     /locus_tag="PK977_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18300.1"
                     /translation="KLWKHEIQKFDQIQDMKYMLDNNTLLFSGVRSGQSDIYVYKIDK
                     QSFEQITNDVYDDLDPSFVAFPNKTGILFSSNRPSAQAASSDDSLPCPRFNIYLVDNW
                     NKSDFKQISRLTDLKMGNARFPSQYNQSHFTFVSDENGINNRYAGFFTTERAGLDTLV
                     FIGDEVLRNPPKKDVDSLLREWNKTDIDSVGFVSITNDSSYVFPLTNYQSSMLETRTA
                     GDNQLVSEVVKLGDVKLLYRLKVDENTLRRRNVTAKATEYRRKLIEEEKKALRKEANT
                     EQTKKDTLTKKQQDFFNTGFEEEKTDENKTKLGDVVKSEEIVKESVLKKAKVYEYRPP
                     KFFNDYLVSGFNNNVLITRYQPYQGGYGPIQLSNGDPFNGIIRVGTSDLFEDWKFAGG
                     FRISPDLKNNEYVLTTQYLKKRIDYGLTYYRNVINNPPLYNDSAAFAKLFTNLYQASV
                     TYPFNRIKSLRFNVAYRSDKYVSLSTDPLSLKATDNRQTYGLAHIEYVHDDAVSPAMN
                     IWHGLRWKVFMDFNARLTTSGAKGPKNHPTTFNFGFDARHYLPIYRNIIWAVRGAGDF
                     SWGNQKIIYYLGGVDNWLMFGQNQKVDDVTYRYFDPSNVPDPDNDYAYQSLAVNLRGF
                     RQNVANGNNALVINSEIRVPVFTSFFNKPINNAFLRNFQIVQFFDLGTAWNGAYDKLE
                     RPSVTYGAPPVLVKIKAGGIGPFAGGYGFGARSTLLGYFLRFDAAWEMNGVFQGKPRY
                     YFAMGLDF"
     gene            3497..5974
                     /locus_tag="PK977_09035"
     CDS             3497..5974
                     /locus_tag="PK977_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF18301.1"
                     /translation="MSSCCRNVLLFCLLLTTLSATSQRKTISGIIKDAHSEEPVPFAS
                     VSFKNTTTGNQSDSAGVFRIVVNDWPSDSLEISCVGYQTFYYKVDISQTNINITITLE
                     RGTFSEGANLKVKVNKGLLVWRKIVQNKPRNDRYRFDNFSYELYNKLELDLKNINFER
                     FGRFKPLRPISELINQNIDTAEGLKYLPTYLSEVLSDYYYQKNPKKRREIIKAVNPNG
                     VKNESVVKMLGGMDQVVNVYNNFIPVFDRQFISPISDNGDYYYNYRVVDTQLIANQRY
                     FHLVFTPRRKGGDTFEGDCWVHVGSFAVQRMNLRLGKEANVNFVENLSLIQEYQLIND
                     STWFLSKDKFVADISPTGKDKPGFIGRKTTTYRNVVVNDSSVIQELSKNKILEEIITL
                     PGSLEKGKEYWQSVRHEPLSKTESSIIKMIDTLTNAPVFQRFTKTMSFIGTGYKDIGN
                     YQIGPWMNWISSNGWEGLRVRFDLGTNKFFDKKIRLHGYLAYGFGDQKLKGMGEIFYL
                     PNKDPRMYLYASFTNDLDWGQNYYGEISQDNVFALAVRKQGVPVKNIKVREKRLEFFK
                     ELFPWMSNKIIVTHKSNLPLRNLVPLDSFRTSASDNPLTSFEVSFRLRFAYLERFLEN
                     HFYRTSLGSIYPIGEIYITRGFPGVFKSSYSYTKISASISDYFKIPPFGNLSWLVYAG
                     KTFGTLPYTLLDVAPGNELYYYNKYAFNMMNRWEFIHDQYAGLNVEHNIGNGIFRLFP
                     KLRLRQFWTAKALWGSLSSENKAYNFKQGHSFQSLDGNTYLELGTGVDNIFKVFRLDF
                     VWRAFPSTLKKEGDKTFGVFGSFRVVF"
     gene            complement(5958..6725)
                     /locus_tag="PK977_09040"
     CDS             complement(5958..6725)
                     /locus_tag="PK977_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18302.1"
                     /translation="MNLSVVILVSKDDAEGTEKTLKSTGGLNAEIILYDTTDTDFAEQ
                     ASRNYNAKLYKGKWEGYDRVRYNAAARAAYDWVLMLHTGEELDEELKSSIRQLELKEP
                     AKAYRIRFRNFYKNKWLRHGEWGKEFHIRLANKRSIRFERQQINENLFYRQNIPVENL
                     HGFISHTIYSDSGQLSVRFKKDALFLAAKYHLQGKKSGVFKMLFSPVCAFLKNYLFKL
                     GILDGGPGYLLSRMHAWHNFLKYARLRKLNKMLRTPL"
     gene            6830..8317
                     /gene="glpK"
                     /locus_tag="PK977_09045"
     CDS             6830..8317
                     /gene="glpK"
                     /locus_tag="PK977_09045"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRF18303.1"
                     /translation="MPHYILSLDQGTTSSRSLVFDKQGNIVSQAQKEFRQYYPQPGWV
                     EHDANEIWSTQFGTMAEAGINMKQIAGIGITNQRETTVVWDKKTGQPVYNAIVWQDRR
                     TAALCDELKSAGKAAMIRQKTGLVIDAYFSATKLKWILDNVSGTRDRAVKGELAFGTI
                     DSWLTWKLTNGEVHVTDVSNASRTMLFNIHTLQWDEELLKLFDIPAAILPEVKPSSKI
                     YGVTGNIIPDSRIPIAGIAGDQQAALFGQLCTQPGMVKNTYGTGCFMLMNTGEKAIAS
                     SNNLLTTIAWQIDGKTEYALEGSVFIAGAVVQWLRDELKIIRTSAEVEKLSAQVKDAE
                     GVYIVPAFAGLGAPHWNQYARGCIFGLTRGSNNAHIARAALDSIAYQTFDVLKAMEAD
                     AGINIAELRVDGGATVNNQLMQFQSDILNCKVVRPVITETTALGAAYLAGLAVGYWKN
                     IAEIKEQWQAEKTFAPALNDEKRKDLVKGWQRAVKASITWADNNE"
     gene            8327..>8771
                     /locus_tag="PK977_09050"
     CDS             8327..>8771
                     /locus_tag="PK977_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18304.1"
                     /translation="MNRSLFIQQLETNSVFDICIIGGGATGLGTALDAAARGLKVALF
                     EQHDFAKGTSSRSTKLVHGGVRYLQQGNIKLVMEALRERGLLLKNAPHLVHNQKFVVP
                     NYKWWEKPYYGIGLKIYDWMAGRLGLGPSQFLSKEETLQLIPALDP"
     assembly_gap    8772..8911
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8939..>9170
                     /locus_tag="PK977_09055"
     CDS             8939..>9170
                     /locus_tag="PK977_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18305.1"
                     /translation="MKDSLTGNEYEVRSKVVINATGVFTDRLLRMDDPKHENIINPSQ
                     GIHLVVDKEFLLSDTAIMIPRTDDGRVLFAVPW"
     assembly_gap    9171..9340
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9383..>9721
                     /locus_tag="PK977_09060"
     CDS             9383..>9721
                     /locus_tag="PK977_09060"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRF18306.1"
                     /translation="MSRDHLIQVAESGLITITGGKWTTYRKMAEEVVDIAIEKNGLEN
                     KPSVTAGMKLSGYDQPVLPADITRESEERLEQLVQHSARTEMCMTVEDFLARRTRQLL
                     LDTNAAIKAAP"
BASE COUNT         2881 a         1935 c         2074 g         2506 t
ORIGIN      
        1 atttagcctg cttgcctctc tttccatttt tgcagccatg gaaactacgg cacaagtaaa
       61 cactgtggag tttgggaaaa accgtgtaca ataccagaaa ttcaaatgga agtattacca
      121 gacagataat ttcaacacct acttcagcca ggatggcctg ggattgggca aatatgtagc
      181 ccaggtggcc gaactggaac taccctccat tgaagaattt gtagaatacg gtttacagcg
      241 ccgcatcaac attgtggtgt acaataacta cgatgaaatg cagcaatcca atatcggcct
      301 ggggattgac tggcaaaaca ccggcggggt taccaaactg gtcaacaata aaatgattgt
      361 ttacttcgac ggcaatcatg ataacctgcg ccgccagata agacagggca ttgccaaaaa
      421 cttagttgac aatttgttat ttggtgacga cttgggagaa tttgcagcca accaggcttt
      481 actggattta cctaaatggc ttaccgacgg ctatattgcc tatgctgcgg aaaattggag
      541 cactacattg gatgatgatc tccgcggagt gatgctggcg ggtgactata aaaatttcta
      601 ccagtttgct tttgcaaagc ccatgctggc gggccatgcc ttctggaagt atatcgccga
      661 caaatacggc aagaacaaaa caacttattt tctttacctg gcccgtattt accgcaatct
      721 gaataacgct tcgaataaaa ttgccaaaaa gaaattcaaa aaaatactgg aagacttcat
      781 gacggatgta cagcaacagt atttcaagga tatccgtgga agaagaaaca caccaagggg
      841 ccagctatca gtaagtgagt ccattggcaa aaaagatttt atccgtttca atgccaaccc
      901 gcttccccgc agttttacct atgccgtagt tgaattcaaa caaggccgct accaggtggt
      961 actcatggag aattttgtta accgccgtgt actgctgaaa ctgggagtaa ggacaagaga
     1021 aaacgaaaaa caccctaact atcctttact ggcttgggat ggcaaaggca caagactggc
     1081 agttttgtac aacgatgaag gaaaaacgaa attctttgtg tatgacgtgg tgaaccnnnn
     1141 nnnnnnnnnn nccaaactct ggaaacatga aattcaaaaa tttgaccaga tccaggatat
     1201 gaaatacatg ctggataata atactctctt attcagcggg gtgaggagcg gccagtcgga
     1261 tatttatgtt tacaaaattg ataagcaaag tttcgaacag atcaccaatg atgtatacga
     1321 tgatctcgat ccttcttttg tagcatttcc caataaaaca ggtatcctgt tttccagcaa
     1381 ccgcccttcg gcacaggcag ccagcagtga tgattcttta ccctgccccc gttttaatat
     1441 ttacctggtc gataactgga ataaatcaga ctttaaacaa atatccaggc tcactgacct
     1501 gaaaatgggt aatgcccgtt tcccctcaca atacaaccag tcgcatttca catttgtaag
     1561 cgacgaaaat ggtatcaata accgctatgc cggtttcttt actacagaaa gagctggttt
     1621 agatacactg gtatttattg gtgatgaggt attgcgtaac cctcccaaaa aagatgtgga
     1681 cagcctcctg agagagtgga ataaaacaga tattgactct gttgggtttg tttctattac
     1741 caacgactcc tcttatgtgt ttccgctgac caattaccag agcagtatgc tcgaaacaag
     1801 aacagcaggc gacaaccagt tggtaagtga agtggtaaaa ctgggtgacg taaaactatt
     1861 atatcgcctg aaagtagacg agaacacact ccgtcgcagg aatgtaacag caaaagcaac
     1921 tgaataccgg cgcaaattga tagaagaaga gaaaaaagcg ttgcgaaaag aagccaacac
     1981 ggaacaaacc aagaaagaca cacttacaaa aaaacaacag gactttttca ataccggctt
     2041 tgaagaggaa aaaacagatg aaaataaaac caagctgggt gatgtggtta agtcggaaga
     2101 gatcgtcaag gaatccgtat tgaaaaaagc caaagtatat gaataccggc ctcctaaatt
     2161 cttcaatgat tatttagtga gtggtttcaa caataacgtt ttgattaccc gctaccagcc
     2221 ttaccagggc ggatacggac ctattcagtt aagcaatggt gatccgttca atggtattat
     2281 ccgtgtcgga acttctgatt tgtttgaaga ctggaaattt gccggtggtt tccgtatcag
     2341 ccccgaccta aagaataatg aatacgttct aactacccaa tacctgaaaa aaagaattga
     2401 ttacggactt acctactatc gtaatgtaat caataatccg ccattatata atgattcagc
     2461 cgcatttgcc aagttgttta ccaaccttta ccaggcaagc gttacctatc cttttaacag
     2521 gatcaaaagc cttcgtttca acgtggcgta cagaagcgat aaatatgttt cactttcaac
     2581 cgacccgtta tccttaaaag caactgataa caggcagact tacgggctgg ctcatatcga
     2641 atatgtacac gatgatgccg tttccccggc catgaatatc tggcacggtc tgaggtggaa
     2701 agtatttatg gatttcaatg cgaggctcac tacttctggt gctaagggac ctaagaacca
     2761 tccgactact tttaatttcg gctttgatgc aaggcattac ctgcccattt accgtaatat
     2821 tatctgggct gttcgtggag ccggtgattt ttcctggggc aatcaaaaga tcatttatta
     2881 tttaggcggg gtcgacaact ggctgatgtt tggccaaaac caaaaagtag atgatgttac
     2941 ctaccgctac tttgatccat ctaatgtacc tgaccctgac aatgattatg cctaccaatc
     3001 cctggccgta aacctgagag gcttcagaca aaatgttgcc aatggtaata atgcactggt
     3061 gattaacagt gagattaggg ttcctgtctt tacctctttt ttcaataagc ctatcaataa
     3121 tgcatttctg agaaatttcc agatagtgca gttctttgac ctgggtactg cctggaacgg
     3181 tgcgtatgat aagctggaga ggccatctgt cacttacggt gctcctcctg tattggtgaa
     3241 aatcaaagca ggcggtattg gcccgtttgc cggcggttat ggttttggtg ccaggagtac
     3301 attattgggc tacttcctcc gcttcgatgc cgcatgggaa atgaatgggg tattccaggg
     3361 caaaccgagg tactattttg ctatggggct ggatttctaa acagataata actttttata
     3421 cccgaatccc ctctaatgag gggatttttt ttctctggcc aacattcaat aactcaagat
     3481 tgtaatttta ccgccgatga gttcgtgttg tagaaacgta ttactttttt gcctgctgct
     3541 gacaacctta tccgctactt ctcaacgaaa aacaatttcg gggataatta aggatgctca
     3601 cagtgaagaa cctgttcctt ttgcttctgt aagttttaaa aataccacta caggtaatca
     3661 atctgattct gcaggtgtat tccgcattgt tgtgaatgat tggccttccg atagtcttga
     3721 aatttcctgt gtgggctatc aaactttcta ctataaagta gacatttccc aaacaaacat
     3781 aaacataact attacccttg aaagaggaac tttcagtgaa ggcgccaacc tgaaagtaaa
     3841 ggtgaataaa ggtttgctgg tatggcgaaa aattgtccaa aacaaacccc ggaacgaccg
     3901 ctatcgcttc gataatttct cctacgaatt atataacaaa ctggaactag acctgaaaaa
     3961 tatcaatttc gaaaggtttg ggaggttcaa acccctgcga cctatcagtg aacttattaa
     4021 ccaaaatatt gatacggcag agggcctgaa atatcttcct acctatttat cagaagtatt
     4081 atccgactat tactatcaga aaaatccaaa gaaaagacgg gagatcatca aagctgttaa
     4141 tcccaacggt gtaaagaatg aaagtgtagt aaaaatgctg gggggaatgg accaggttgt
     4201 aaatgtatac aacaacttta tacccgtatt tgatagacag tttatcagcc cgatcagtga
     4261 taacggtgat tattattaca attaccgggt ggtggacaca cagttgattg ccaaccagcg
     4321 gtactttcat cttgtcttca cacctcgacg gaagggaggt gatacttttg aaggcgactg
     4381 ctgggtgcat gtaggcagct tcgctgttca gcggatgaac cttcggctgg gaaaagaagc
     4441 aaatgtaaat tttgtggaga accttagcct gatacaggaa taccagctca tcaatgactc
     4501 cacctggttt ttaagcaagg acaaatttgt ggctgatatt tcacccaccg gaaaagataa
     4561 gcccggtttt atcggccgca aaacaaccac ttatcggaat gtagtagtaa atgatagctc
     4621 tgtcatacaa gaactttcaa aaaataaaat actggaagag atcattacgc ttcctggttc
     4681 attggaaaaa ggaaaagaat actggcaatc tgtacggcat gaaccgctca gcaaaacaga
     4741 aagcagtatt atcaaaatga ttgatacatt aacgaatgca ccggtatttc agcgcttcac
     4801 aaaaaccatg agctttattg gtacagggta caaagacatt ggcaactacc agatcgggcc
     4861 atggatgaat tggattagtt ccaacgggtg ggaaggactg agggttcgct ttgacctggg
     4921 taccaataaa ttcttcgaca aaaagataag gcttcatggt tatctcgcct atggattcgg
     4981 cgatcaaaaa ctaaaaggaa tgggtgagat tttttatttg cccaacaaag atccaaggat
     5041 gtatttatat gcttcattta ccaacgatct ggattgggga caaaattatt atggcgaaat
     5101 ctcacaggac aatgtgttcg cattggctgt tcgtaaacaa ggtgtgccgg taaaaaacat
     5161 caaggtgagg gagaagcggc tggaattttt taaagagctg tttccgtgga tgtcaaataa
     5221 aatcattgta acgcataaat ccaatctgcc actgcgaaac ctggttccgc tggattcttt
     5281 cagaacttca gcctcagata acccgttgac cagttttgag gtctcttttc gattgcgttt
     5341 tgcttactta gaaagattcc ttgaaaatca tttttaccga accagtctcg gcagcatcta
     5401 ccctattggt gaaatttata ttacccgtgg cttcccgggt gtattcaaaa gcagttacag
     5461 ttacacaaaa atttcagcca gtatttccga ctattttaaa atcccgcctt ttggcaacct
     5521 ctcctggctc gtatatgcag gaaaaacatt cggcaccctg ccttatacac tgctcgatgt
     5581 tgcaccggga aatgaattgt attactacaa taaatatgca ttcaatatga tgaaccggtg
     5641 ggagtttatc catgatcagt atgcaggtct taatgtggag cacaatattg gcaacggcat
     5701 attccgtctt tttcctaaac taagactccg ccagttctgg acggccaaag cattatgggg
     5761 aagtctgtca tcagaaaaca aggcttataa ttttaaacag gggcacagtt ttcagtcact
     5821 ggatggaaat acttacctgg aactgggtac cggggtagat aatatcttca aggtatttcg
     5881 tcttgatttt gtctggaggg cattcccttc tacattgaaa aaggaaggcg ataaaacatt
     5941 tggtgtgttt ggaagcttca gagtggtgtt ctgagcattt tgttcagctt cctgagcctt
     6001 gcatatttaa ggaaattatg ccaggcatgc attcttgata acaaatagcc cgggccgcca
     6061 tcaagaatgc ccagtttaaa aagataattt tttagaaagg cacaaaccgg tgaaaacaac
     6121 attttaaaaa caccggactt cttgccctgc agatgatatt tagccgcaag aaaaagggca
     6181 tcttttttga atctaacgga cagttggccg ctatcgctgt aaatagtatg agaaataaat
     6241 ccgtgcagat tttcaactgg aatattttgc cggtaaaaca agttttcatt tatctgttgc
     6301 ctttcaaacc tgatgcttcg tttattcgcc agcctgatat gaaactcttt gccccattca
     6361 ccgtgccgca gccatttatt cttataaaaa tttctgaacc ggatacgata cgctttagca
     6421 ggttctttta attcaagttg cctgatggaa gattttagtt cttcgtccag ttcttcgccg
     6481 gtatgcaaca tcaataccca gtcatatgct gcccttgcag cagcattgta ccttacacgg
     6541 tcatatccct cccatttccc tttgtaaagc ttcgcattgt aattacgaga tgcctgctcc
     6601 gcgaagtcag tatcagttgt atcatataaa attatttctg cgttaagtcc gccagtactc
     6661 ttcagcgtct tttcggtgcc ttccgcatca tctttagaaa ccaggatcac aactgacaag
     6721 ttcatattga ggctttcaat acaagttaat atggcattct tcaaaaacac ttgacatttg
     6781 tcaagttgat actattttca gtaattttct catcataata aaataagcca tgccacatta
     6841 cattctttct ttagatcagg gcaccaccag cagccgtagt cttgtttttg ataaacaggg
     6901 taacatcgta agccaggcgc agaaagagtt caggcagtac tatccgcaac cgggctgggt
     6961 agaacatgat gccaatgaaa tatggagtac gcaatttggc actatggcag aagccggcat
     7021 taatatgaaa caaattgcgg gtattggcat taccaaccaa cgggaaacaa ccgtagtatg
     7081 ggacaaaaaa accgggcaac ctgtctataa tgcaatcgta tggcaggaca gaagaacagc
     7141 cgcattatgt gatgaattaa aatcagcagg aaaggctgca atgattcgcc aaaaaacggg
     7201 gctggttatt gatgcctatt tttctgcgac aaagcttaaa tggattctgg ataatgtatc
     7261 cggtacaaga gacagggctg taaaaggcga attggctttc ggtactattg atagctggct
     7321 gacatggaaa cttaccaatg gagaagttca tgtaaccgat gtatccaacg cctcaagaac
     7381 aatgctcttt aatattcata ctttacaatg ggatgaagaa ttgctgaaat tatttgacat
     7441 cccggctgct atactgccgg aagtaaaacc aagcagcaag atctatgggg taaccggtaa
     7501 tatcattcct gactcaagaa tacccattgc aggaatcgca ggcgaccagc aggcagcatt
     7561 attcggtcag ctttgtactc aaccgggcat ggtaaagaat acgtatggca caggttgttt
     7621 tatgctgatg aatacaggag aaaaagcgat cgcttccagt aataatttac ttaccactat
     7681 agcctggcaa attgacggaa agacagagta tgccctggaa ggaagcgtat tcatagcagg
     7741 agcagtagtg caatggctgc gggatgaact gaagatcatc cgcacttcgg cagaagtaga
     7801 aaaactatct gcacaggtaa aagatgccga aggcgtatat attgttcctg catttgcagg
     7861 cttgggtgca ccacattgga atcaatatgc aagaggctgt atattcggtc tgacccgggg
     7921 aagtaacaat gcacatattg cccgggcagc tttagacagc atcgcctatc aaaccttcga
     7981 tgtgttgaaa gcaatggaag ccgatgccgg tatcaatatt gctgaactaa gagttgacgg
     8041 tggtgcaaca gtcaataacc agttgatgca gtttcaaagt gacattctga attgtaaagt
     8101 ggtgcggcct gtcataacag aaaccacggc attgggggct gcctatcttg ctggactggc
     8161 tgtgggctat tggaaaaaca ttgcagaaat taaggaacaa tggcaggctg aaaaaacctt
     8221 tgcacctgca ctgaatgatg aaaaaagaaa agatctggtg aaaggctggc aaagggcggt
     8281 aaaagcttct attacctggg ctgataataa tgaataatac ggccccatga accgttcctt
     8341 attcatacaa caactggaga cgaattcagt attcgacatc tgtatcattg gtggtggtgc
     8401 cacaggcttg ggaactgcat tggatgcggc agcaagggga ttaaaggttg ctctctttga
     8461 gcaacatgac tttgccaaag gcacttcttc ccgaagtacc aaactggtac atggcggagt
     8521 ccgttacctg caacaaggca atatcaaact ggtgatggaa gcactcaggg aaagaggtct
     8581 tctgttaaag aatgcaccgc acctggtgca taatcagaag tttgttgttc ccaactataa
     8641 atggtgggag aaaccatact atggcatcgg gctcaaaatc tatgactgga tggctgggcg
     8701 gctgggtctg gggccctctc aattcttatc aaaagaagaa acgctgcaat taattcctgc
     8761 cctggatcct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgcaaaaa aagatttgcg gggcttgggt
     8941 gaaggattcc ctaaccggca atgaatatga agtaagaagt aaagtggtca tcaatgccac
     9001 cggtgtgttt acagaccggc tgctgagaat ggatgatcca aaacatgaaa acattattaa
     9061 cccgagccag ggtattcatt tggtagtgga caaggaattt ttgttgagcg atacagcaat
     9121 catgataccg agaacagatg acggcagagt cttgtttgct gtcccctggc nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgaggccat tagtaaaagg
     9361 aaaggctaaa aaaacagcct ccttgtctag agatcatttg atacaggtcg ctgaatccgg
     9421 gctgataacc attacaggtg gcaaatggac aacttatcgt aaaatggcag aagaggtagt
     9481 ggatattgcc atagaaaaaa acggacttga aaacaaacca tctgtaacgg caggaatgaa
     9541 actatcagga tatgatcaac cggttttgcc ggcagacatt accagggaaa gtgaagaaag
     9601 actggaacaa ctggttcaac attcggccag gactgaaatg tgtatgactg tagaagattt
     9661 tttagcaaga agaacaagac aattgttgct cgatacaaat gctgccatca aagcagctcc
     9721 t
//
LOCUS       DAORLS010000101         9716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3758_length_9716_cov_1.964393, whole genome shotgun sequence.
ACCESSION   DAORLS010000101 DAORLS010000000
VERSION     DAORLS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3758_length_9716_cov_1.964393"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..213
                     /locus_tag="PK977_09065"
     CDS             43..213
                     /locus_tag="PK977_09065"
                     /inference="COORDINATES: protein motif:HMM:NF023786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18307.1"
                     /translation="MQCDECAAKYEGCCSKECQDTIHLPSERRKELRKGIDKGQHIFN
                     KSRQRLFPRGHS"
     gene            complement(219..1331)
                     /locus_tag="PK977_09070"
     CDS             complement(219..1331)
                     /locus_tag="PK977_09070"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HRF18308.1"
                     /translation="MKILVLLCVFFLSKPSVCQEFYLLIGTYDSPGSEGIYIYRFNAD
                     NGTAKPVRHIKTSNPSYLTISNDERFVYSVNENGKNGNGGEVTSFSFDKKNGRLSYVN
                     TQSSGGDHPCYIEMDKTGKWVFAGNYSSGNLAVFPVRKDGGLSSADTLIQHTGQGINE
                     KRQKGPHVHCTVLSPDNKWLLVPDLGIDKTMIYRFDAGTGKLSTGKQKYIASDPGAGP
                     RHFVFHPNNKVGYLIEELSGTVAAHKFKQGRFKRIQRVSTLVEGDSSFAGSADLHISP
                     DGKFLYASNRGNINNIAIFSLNNKGKLNLIGHQPTLGNTPRHFNFDPTGTYLLVCNQN
                     SNEIVIFKRDINTGQLTDSGKRIPIGKPVCIKWIMP"
     gene            1466..1894
                     /locus_tag="PK977_09075"
     CDS             1466..1894
                     /locus_tag="PK977_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18309.1"
                     /translation="MKKIFTLPVLSLLALVILLSSCFRDTTIVSSNENYWLSKEQGEV
                     VYSDSYCNYYVVETYYGYNIVRSYGGYKPFEGSIVYGNFSSQGTRDMYNYSTGVVFTG
                     TVTDYWLTYNEAQDALDYYCPLYGKGTKREFKTSTVIKKK"
     gene            complement(1941..>2472)
                     /locus_tag="PK977_09080"
     CDS             complement(1941..>2472)
                     /locus_tag="PK977_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRF18310.1"
                     /translation="PIITPSTKASEGHDEDISPEEIIDKGLATREEWEVLSNYALRLF
                     ERGKEIAAKRGLILVDTKYEFGKLENEIVLMDEIHTPDSSRYFYAEGFEDRQKKGERQ
                     KQLSKEFVREWLIANNFMGKEGQTVPEMTDDWVETISKRYIELYEKVIGKPFIPEKIS
                     DDDLYNRVTNCLSTLI"
     assembly_gap    2473..2500
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2501..2880)
                     /locus_tag="PK977_09085"
     CDS             complement(<2501..2880)
                     /locus_tag="PK977_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRF18311.1"
                     /translation="MSQFSFPGQTGFYRGKVRDVYSIGDQWLVMVASDRISAFDVILP
                     RPVPFKGQVLNQIAAYMLNATKDICPNWLVSVPAPNVSVGKKCVPYKIEMVVRGNLTG
                     HAWRTYASGKRILCGAVMQEGMKE"
     gene            3030..>4790
                     /locus_tag="PK977_09090"
     CDS             3030..>4790
                     /locus_tag="PK977_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF18312.1"
                     /translation="MRKSYSFLAAWLMACLISFSAYAQSITISGTVKNSTTKEGVPAV
                     SVAVKGTSRGTYTNSDGEFTLTVPKLPVVLVFTSVGFDSQEVTVSDASQPVAVDFKVN
                     TALGQEVVVAAARAPQRLLESPVTVERLSTTALRNMPAPNYYEAIGNLKGVDVHTASL
                     TFRTITTSGFVSSGNLRLNQLIDGMDNQAPGLNFSVGSVVGPSDLDVDNVEVLSGASS
                     ALYGSGGMNGTVLVTTKNPFKYQGLSFNVKQGIMHVDGKQRNAAPFYDWNFRWAKAYK
                     NKFGIKLAAQLVKGSDWEAEDYRNKQQIGILSKVVGGNRQNDPNFNGINIYGDETSAD
                     MNGFAYFVQDQTRRQILAASSVIIPGGFDVVNAANTYFTAIGNPQYPTNAQISTFITS
                     LAALGPTAQAAVQQMMPFYIGLRNNYFNKASVSRTGYQEDQLVDYNTMNFKLTAGLHY
                     KITNNLEVSLNSYLGTGTTVYTGADRYSLRNLKMAQHKLEFRSKNWFIRGYTTQENAG
                     DSYNASALGAFLNESYRASSAWFPLYIGTFSEGRRLNGVAGQFPSDFQLHQAARASAD
                     VGRWVPGTKAFDSVAAVIKKT"
     assembly_gap    4791..4806
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4807..>5186
                     /locus_tag="PK977_09095"
     CDS             <4807..>5186
                     /locus_tag="PK977_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18313.1"
                     /translation="ALFLDKSDLWASDAQFNISEIFKFSKVIDVVAGVSWKQWVMNSQ
                     GTIFADTNQVIRINEYGAFIQLKKSLFNDYLTLSASGRFDKQTNFDPRFTPRFTAVLK
                     VVKDNFIRLSYQSAYRFPSHQNQY"
     assembly_gap    5187..5282
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5283..>5566
                     /locus_tag="PK977_09100"
     CDS             <5283..>5566
                     /locus_tag="PK977_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18314.1"
                     /translation="GYTAASVLAYRSGTLADSSRLVRAQYNPVTPETVKSYELGYKGV
                     IGKKLLVDAYVYYSRYANFLVSEAVVQSRNGANFELYSPYSSTNVSYVQN"
     assembly_gap    5567..5579
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5580..>5922
                     /locus_tag="PK977_09105"
     CDS             <5580..>5922
                     /locus_tag="PK977_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18315.1"
                     /translation="VIKSQGWGIGVEYQAIKNYFFYGNVFSDYLKNVDPSVVTFFNAP
                     KYRFNVGLRNDNAYKNIGFNVVFKWQDNNYYEGTFVSGTLPYFGWWDAQVTYRPQGTK
                     SVFRIGGTNLGN"
     assembly_gap    5923..5960
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6116..7042)
                     /locus_tag="PK977_09110"
     CDS             complement(6116..7042)
                     /locus_tag="PK977_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18316.1"
                     /translation="MKDTKSLLLLMLSVGLTGTWVYHLYDKTQYTKIRREIFVKDSLA
                     VAQGVQDSLHKIYSQTIDELDARLDSSRTSAGLLKGELSNKLAEIYRLRTEIAAILKK
                     NDVKREDLALARKKTIELQQLVQELQSKNYTIEEEKQQISATLDKVNIQVKSLEGNMQ
                     ELTRENKALAEKVSLASTFVTTELNLVPVTLKRDKELETNSASKTSKLVVSFGVKNNI
                     SDYDNAELYIVVNQPDGNVMTIDAWESSSTIDTRNEGKKRYTRKIKFEYQKGENKKLS
                     FSLNPEDYQKGTYTVQLYHNGLMIGQTSKTLN"
     gene            complement(7057..7536)
                     /locus_tag="PK977_09115"
     CDS             complement(7057..7536)
                     /locus_tag="PK977_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18317.1"
                     /translation="MIRAWQQLFRVLAAAENSEIIYAVNASGAYPLSDNIAVSGGIAW
                     AIATLTHPELIDNPVVSVTFDEIRKFSEKLYTNFPVYSASFLAGNINRANEKDAVIKE
                     VTRVHQVFDQKSLMAGCGLLLKIMRQFESAFDTKHFYLVKNGQVGWVSAYVDQQLTK"
     gene            complement(7463..8191)
                     /locus_tag="PK977_09120"
     CDS             complement(7463..8191)
                     /locus_tag="PK977_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18318.1"
                     /translation="MIISKFCRQLGTVSFLLICTTAFTQSSIKTKYSNSTVYSGIEVG
                     SKGVKMSVIEVGKNAKSSGAIAILKDSSVNTDFISFTDETFSATLNGLTGLFNKAVKD
                     YQIPSSKIFTVLSSGVKMQAEKDQKTAWMQRLIDSFRIRINEPQRQVEVVDVLQEARL
                     SHLGIVPDSRRFTTFLIDIGSGNTKGGFFPYGNTKDFKLFQVNWGTKSTTNETDKRCE
                     NDKSLAAIIQGTGGSRKFRNYLCC"
     gene            complement(8350..>9390)
                     /locus_tag="PK977_09125"
     CDS             complement(8350..>9390)
                     /locus_tag="PK977_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18319.1"
                     /translation="KYLAAGFSNPYKIIYKKSKFLNMAETNYPSATGSTPVQPAKNNN
                     SKNIIIGALAAVVLGSWGYFLWDKNKSSEKISNQQTQITSLDSAKTEIQMEFDNALAR
                     LDSVTGANNNLQGQLADRQKEIDAKKAEIRKILNDKNATQSQLAKAKSMINELNEKIS
                     TLEAEVARLTGENEQLTADNTTLKQEKSDLEQNLTLTKTEKENLEKTVDVGSTFSASN
                     IQVTPVNEKKGGKEKNTTTAKRVDKLVVSFDVENRIAKSGPADMYLIVTAPDGKIISE
                     NGNVLNTRTDGDKNFTAKIPVNYEQGTRKSVQFPIQQNDFMTGNYKVEIYHNGFKIGE
                     GVRSLKKGGLFG"
     assembly_gap    9391..9424
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2633 a         1954 c         1897 g         3007 t
ORIGIN      
        1 acccatacca attgtaaaaa cgacggttgc cacctgcttt tcattcaatg tgatgaatgt
       61 gctgcaaaat atgaaggctg ttgcagtaag gaatgccagg ataccattca tcttccgtcc
      121 gaaagaagga aagaactccg gaaggggatt gataaagggc aacatatttt taacaaatca
      181 aggcaacggc tgtttccccg aggccatagt tgatctcatc agggcattat ccatttgatg
      241 caaaccggtt taccaatagg tattctttta cctgaatcag taagttgccc tgtatttata
      301 tctcttttaa agatgacaat ctcattactg ttttgattac aaaccaaaag gtatgtacca
      361 gtcgggtcaa aattgaaatg acggggagtg tttcccaggg ttggctggtg tcctatcaga
      421 ttgagcttac ctttattatt taaagagaaa atcgcaatat tatttatgtt tccccggttg
      481 gaggcgtata aaaatttccc atctggtgaa atgtgtaaat cagcgcttcc ggcaaagcta
      541 ctgtcgcctt caaccaaagt ggaaaccctt tgtattcttt taaatcttcc ttgtttaaat
      601 ttgtgtgctg ctacagtacc gcttaactct tcaataaggt atcccacttt attattggga
      661 tgaaaaacaa aatgccttgg tccggctccc ggatccgatg caatatattt ttgtttaccg
      721 gttgacaatt tacctgtgcc ggcatcaaaa cgataaatca tggttttatc aataccaaga
      781 tctggtacca gcaaccattt attatccggc gaaaggaccg tacaatgaac atgtggccct
      841 ttttgccgct tttcatttat gccttgccct gtatgttgaa tgagagtgtc tgcggaactt
      901 aatccgccat cttttcttac cggaaagaca gcgagattgc cagagctata gttgccggcg
      961 aacacccatt tcccggtttt atccatttca atgtaacaag ggtggtcacc tccagatgac
     1021 tgtgtattaa cataagatag ccggccattc tttttatcaa atgaaaagga tgtgacttcg
     1081 cctccgtttc cattctttcc gttttcgttc acagaataaa caaatctttc gtcatttgat
     1141 atagtgagat aggaggggtt agaagtcttt atatggcgga ctggtttagc ggttccatta
     1201 tcagcattga agcgataaat atatatgcct tcgctgccgg gtgaatcata tgtgcctatc
     1261 agcaggtaaa actcctggca tactgaaggt tttgataaaa agaaaacaca gagtaatact
     1321 agaatcttca taacatttct ttcagaaagt gaatatctct tattaaaatg attttaacaa
     1381 acctgtgcat gctttggcaa ggtctggcac aatcttatca ttgtagtaat cggaaacaga
     1441 tttttaaact atcaaatcaa aagccatgaa aaagatattt actcttccgg ttctttcatt
     1501 attagctctt gtaattttac tcagcagttg ttttagggat acaactattg taagcagtaa
     1561 tgaaaactat tggttgtcta aagaacaagg cgaagttgtg tacagtgaca gctactgcaa
     1621 ctattatgta gttgaaacct attatgggta caatattgtt cgcagctacg gcggttataa
     1681 gccttttgaa ggatcaatag tttatggaaa cttcagcagc cagggaactc gtgacatgta
     1741 caactattca acaggggtgg tctttaccgg tacagtaacc gattattggt taacatataa
     1801 tgaagcccag gatgccctgg attactattg ccctctctac ggaaaaggca ccaaaagaga
     1861 attcaagaca tcaacagtta ttaagaaaaa ataactgaca gagataaact taaaggagcg
     1921 gtttaacaaa ccgctccttt ttatattaaa gtactaaggc aattagtcac tctattatat
     1981 aaatcgtcat cactgatttt ttcaggtata aaaggtttgc caattacttt ctcataaagc
     2041 tcaatatacc ttttggaaat agtttccacc cagtcatcag tcatctccgg aacagtttgt
     2101 ccttctttgc ccataaagtt gttagctatc agccattccc tgacgaactc tttacttaac
     2161 tgtttttggc gttctccctt tttctgacgg tcttcaaaac cttctgcata aaaatacctc
     2221 gaggaatcgg gggtgtgtat ctcatccatc aatacaattt cattttccag cttcccaaat
     2281 tcgtatttgg tatctaccaa tatgagcccc cgttttgctg cgatttcttt gcctcgttca
     2341 aagagccgga gagcatagtt gcttaatacc tcccattcct ccctggttgc aagtccctta
     2401 tcaatgattt cctccggtga aatatcttca tcatgcccct cagaggcctt ggtgctgggg
     2461 gtaatgatag ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttctttca ttccttcctg
     2521 cataaccgca ccacaaagta ttctcttgcc tgaagcatat gttcgccagg catgtcccgt
     2581 cagatttccc cgaactacca tttctatttt ataaggcaca catttttttc cgacggaaac
     2641 atttggggcg ggaacgctaa ccaaccagtt gggacaaata tcctttgttg catttagcat
     2701 atatgcagca atctggttta aaacctgtcc tttaaagggt acaggacggg gaaggataac
     2761 atcaaaagct gagatcctgt cagaagcgac cattaccagc cattggtcac cgatactata
     2821 cacatcccgg accttgccgc ggtaaaaacc ggtttggcca ggaaaggaaa attgactcat
     2881 aaaccgaata taggacgggc ctaaaaaccg gccaatctgg gggtgtttaa gatttcaaca
     2941 atttatcctt tccccttaaa aattaaattt ttagataaac tttacaatgc ttatttttcc
     3001 gccaaattcc aaaactaaaa atccctatta tgagaaaaag ctatagcttt ttggctgcat
     3061 ggttgatggc ttgccttatt tcttttagtg cttatgcaca atccataacc atttcaggta
     3121 cagtaaaaaa cagcaccacc aaagagggtg tacctgctgt atctgtagcc gtaaaaggta
     3181 ccagccgggg tacttacaca aattccgatg gtgaattcac ccttacagta cccaaacttc
     3241 ccgtagtatt agtatttact tctgttggtt ttgacagcca ggaagtaacc gtatccgatg
     3301 cctctcagcc tgttgcggtt gattttaaag tgaatactgc gcttggacaa gaagtagttg
     3361 ttgctgctgc ccgtgctcca cagcgtttac tggagtctcc cgttacggta gaaagactca
     3421 gtacaactgc tctccgtaat atgccggcgc ccaactatta tgaggcaatc ggaaacctga
     3481 aaggtgtaga tgtgcatact gccagtttaa ctttccgaac aattactacc agtggatttg
     3541 ttagttcagg taaccttcgt ttgaatcagt tgattgatgg aatggataac caggctcccg
     3601 gtttgaactt ttctgtaggt agtgtagtag gaccctctga tctggatgta gacaacgtag
     3661 aagttctctc cggcgcctct tcagcattgt atggctctgg tggtatgaac ggaactgtat
     3721 tggttacgac aaagaatcca ttcaaatacc agggtctaag cttcaatgtc aagcaaggta
     3781 ttatgcatgt tgacggaaag cagcgtaacg cagctccttt ctatgactgg aatttccgtt
     3841 gggcaaaagc ttacaaaaac aaatttggta taaaactcgc agcccagtta gtaaaaggta
     3901 gcgattggga agctgaggat tacagaaata aacaacagat cggtatttta agtaaagtgg
     3961 tgggcggcaa ccgccaaaat gaccccaact ttaatggtat caacatctat ggagatgaga
     4021 ccagtgctga tatgaacggt tttgcctatt tcgtacagga tcaaaccaga aggcaaatac
     4081 ttgctgcctc ttccgttatc atacctggag gttttgatgt cgttaatgct gctaatacat
     4141 atttcacagc tattggcaat cctcaatacc ctacaaatgc gcaaatctcc actttcataa
     4201 cttctcttgc tgcgttgggc cctacagcac aggctgccgt acagcaaatg atgcctttct
     4261 atattggttt aagaaataat tactttaata aagctagtgt ttcaagaact ggttaccagg
     4321 aagatcaact ggttgattac aatacaatga acttcaagct gacagctggt ttacattata
     4381 aaataaccaa taacctggag gtttctttga acagctacct cggtaccggt acaactgttt
     4441 acacaggtgc tgaccgttat tctctgcgta accttaagat ggcacagcat aaattggagt
     4501 ttcgttctaa aaactggttt atccgtggtt acacaaccca ggaaaatgca ggtgattcat
     4561 acaatgcatc agcattgggc gcattcctta atgaatcata cagagccagc tctgcctggt
     4621 tcccgcttta tattggtact ttctcagagg gacgcaggtt gaatggtgtt gctggtcaat
     4681 tcccaagcga tttccaactg catcaagccg caagggcttc tgctgacgtt ggtcgctggg
     4741 tgcccggaac aaaggccttt gattctgtag ctgccgtgat taagaaaacc nnnnnnnnnn
     4801 nnnnnngagc cttattcctg gataaatcag atttatgggc ttctgatgca cagttcaata
     4861 tttctgaaat attcaaattc tctaaggtaa ttgatgtagt agctggtgtt agctggaaac
     4921 aatgggtaat gaattctcag ggtaccattt ttgctgatac taaccaggtt atcaggataa
     4981 acgaatatgg tgcatttatt cagttaaaga aaagcctgtt caacgattat cttacattga
     5041 gtgcatcagg ccgtttcgat aagcaaacaa attttgatcc acggtttact ccaaggttta
     5101 ctgcggtgtt aaaggttgta aaagacaact ttatccgtct ttcttatcag tcagcttacc
     5161 gcttcccatc ccaccaaaac caatatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnccggctat actgcagcaa gtgtattagc ttaccgttcc ggtacactgg cagattcaag
     5341 ccgcctggtt agagcacagt acaaccctgt aacaccggaa actgtgaaat catatgaatt
     5401 gggttataaa ggtgtaattg gtaagaaatt actggtggat gcttatgtat actacagccg
     5461 ttatgctaat ttccttgtta gcgaagcagt ggtacagtct cgtaacggag ccaattttga
     5521 attgtactct ccatacagca gtaccaacgt ttcttatgta caaaacnnnn nnnnnnnnng
     5581 ttataaaatc ccagggctgg ggtataggcg tagaatacca ggcgattaag aattactttt
     5641 tctatggaaa cgtattctct gattacctta aaaatgttga tccaagtgtt gttacattct
     5701 tcaatgcacc taaataccgt ttcaacgtag gtttgaggaa cgataatgct tataagaata
     5761 taggctttaa cgtggtattc aaatggcagg ataataacta ctatgaagga accttcgtta
     5821 gtggtacact tccttacttc ggatggtggg atgcccaggt tacttaccgt ccccagggta
     5881 caaagagtgt atttcggata ggtggaacta accttggtaa ctnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn ggggcttata ctatatgagc tatggttaca acctgtttta
     6001 ataattgata ttgggttata agtataacta atcccccgca tcagcggggg attcttattt
     6061 gtattaaatt cttagactaa tgggtgagag tggctaagaa gagaaaagta caagcttagt
     6121 ttagtgtttt tgaagtttgc cctatcataa gcccattatg atataactga actgtatatg
     6181 tccctttttg gtaatcttcg gggttcagtg agaaggagag ttttttgttt tccccctttt
     6241 gatattcgaa ttttatcttc cgggtgtacc gctttttccc ttcattgcgg gtatctattg
     6301 tagaagaaga ttcccatgca tcaatagtca ttacattgcc atcaggctga ttaaccacaa
     6361 tataaagctc tgcattgtca taatcgctga tgttattttt tacgccaaat gataccacca
     6421 atttgcttgt tttggaggca gaatttgttt caagttcctt atctcttttt aaagtaactg
     6481 gcacaaggtt gagttcggta gtgacaaagg tggaggctag gctcactttt tctgccagcg
     6541 ctttgttttc ccgggttaat tcctgcatat tcccttccag gctttttacc tggatattta
     6601 ctttatcaag ggtggcagaa atttgctgct tttcttcttc aattgtataa tttttacttt
     6661 gtaactcctg aaccagttgt tgcagttcaa tagttttctt tcgggccaat gccaggtctt
     6721 ctcgttttac gtcattcttt ttcaggattg ccgctatctc tgttcttaat cggtaaattt
     6781 cagccagttt gttacttagc tcgcctttaa gtagacctgc tgatgttcgg gaagaatcaa
     6841 gccgggcatc aagttcatca atagtttgtg aatagatttt atgaagggaa tcctgcacac
     6901 cctgtgccac agccagagaa tcttttacaa aaatctccct cctgattttg gtgtattggg
     6961 tcttgtcgta aagatgataa acccaagtgc ccgttaaccc aactgacaac ataagcagca
     7021 aaaggctctt ggtatctttc attacaatta aactacttat ttggttaact gctggtcaac
     7081 atacgccgaa acccatccaa cctgcccatt ttttacgaga tagaaatgtt ttgtgtcaaa
     7141 ggcagattca aattgcctca ttatcttgag caaaaggcca caacctgcca tcagtgattt
     7201 ctgatcaaaa acctggtgaa ccctagtcac ctcttttatc actgcatctt tttcattggc
     7261 acggttaatg tttcctgcaa ggaaagaagc agaatatacc gggaagttag tgtaaagttt
     7321 ttcggagaat tttctgatct catcgaacgt tacagatact acagggttgt ctattaattc
     7381 aggatgggta agagtggcaa ttgcccaagc tatgcctcca ctaacagcaa tattatcact
     7441 caaaggataa gcgccgctgg cattaacagc ataaataatt tctgaatttt ctgctgccgc
     7501 cagtaccctg aataattgct gccaggctct tatcattttc gcatctttta tccgtttcat
     7561 tggttgtgct cttagtgccc caattaacct ggaagagttt aaaatctttt gtattcccgt
     7621 aagggaaaaa gcctcctttg gtattaccac tgccaatatc gatcaaaaaa gtagtaaacc
     7681 tgcggctatc aggtacaatg ccaagatgtg aaaggcgagc ctcctgtaat acatcaacta
     7741 cctctacttg acgctggggt tcatttatcc ttatcctgaa tgaatcgata agtctttgca
     7801 tccaggcagt tttctggtct ttttcagctt gcattttaac accgctgctg agaactgtaa
     7861 atattttact ggaaggtatt tgatagtcct taaccgcttt attaaacaac ccggtaagac
     7921 cattaagggt ggctgaaaat gtttcgtcgg taaaactgat gaaatcggta ttaacggaac
     7981 tatctttcag aatggcgata gccccgctgc ttttggcgtt cttacctact tcaattacac
     8041 tcatcttcac tcctttagag ccgacttcaa taccagaata aacagttgaa ttactgtatt
     8101 tggtcttaat actgctttgt gtaaaagcag tcgtacagat aagcaaaaaa ctaaccgttc
     8161 ccagctgtct gcaaaacttt gatattatca tggtgagttt attggttgaa taaaattact
     8221 gcattggtaa gcagtaatta tgccttcttg tctcaccgga gtaatatgtg taaaagatgt
     8281 gtgtttcact gtaaaacaaa aagccgtctg atgaatcagg acggcttttg ttgcaggagt
     8341 taactgcttt taaccgaata aaccaccttt tttcaggctt ctcacacctt cgccgatttt
     8401 gaagccatta tgataaattt caactttata attacctgtc atgaagtcat tttgctgaat
     8461 cgggaactga actgatttac gggtgccttg ttcataattc accggtatct tagcagtaaa
     8521 gtttttatcg ccgtctgtac gggtattgag cacattgcca ttttcagaaa ttattttacc
     8581 gtcaggggct gttacgataa gatacatatc cgccggcccg gatttggcta tacggttctc
     8641 cacgtcaaaa gaaacaacaa gcttgtctac tcttttagcg gtagtggtat ttttctcttt
     8701 accacctttt ttctcattaa caggagtaac ctggatgttg gatgcgctaa atgtagaacc
     8761 tacatcaact gttttctcga gattctcttt ttcggtcttt gttaaggtga ggttttgctc
     8821 aaggtctgat ttttcttgct ttaatgtagt attatcagct gtcaactgct cattttcacc
     8881 ggtcagccgt gctacttctg cttccaatgt agagattttt tcattcagct cattaatcat
     8941 cgatttggcc ttggccaatt gtgattgagt ggcgtttttg tcatttaaaa ttttacgtat
     9001 ctcagccttt ttagcatcaa tttctttttg cctgtcagcc aactggccct ggagattgtt
     9061 gttggcgcca gtcacagaat caaggcgtgc cagcgcatta tcaaactcca tttgaatctc
     9121 tgtttttgct gaatcaagag atgttatctg tgtttgctga ttggaaatct tttcactgga
     9181 cttattcttg tcccataaaa agtaacccca tgatcccagt accactgcag ccagggctcc
     9241 aatgatgatg tttttggaat tgttgttctt tgccggctga accggtgtac ttcctgttgc
     9301 ggaagggtag tttgtctctg ccatattaag gaatttagat tttttgtaaa tgattttata
     9361 aggattactg aatccggctg caaggtactt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnncatccg ttttttttat ttcctgttaa aaaaacttat ctgggcactg aaatttctaa
     9481 aaccgtgcca gaaaatccgg cacctttttc acaaccgtgg atttttattg ctatatccaa
     9541 gcctgaaagc ctgtaattta gtggcttatg agtgtgcaaa agatattaga tgattggaaa
     9601 aagaactctt ttaagccaat ttactggttg gaaggggaag aggagtattt tattgacaag
     9661 gtcatcgatt atgctgaaca taatattctg ccggaaagtg aggcttcatt caatct
//
LOCUS       DAORLS010000102         9611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3814_length_9611_cov_1.662934, whole genome shotgun sequence.
ACCESSION   DAORLS010000102 DAORLS010000000
VERSION     DAORLS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3814_length_9611_cov_1.662934"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            677..1891
                     /locus_tag="PK977_09130"
     CDS             677..1891
                     /locus_tag="PK977_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18320.1"
                     /translation="MLERANPSIAADLFAPGRMTFEDLYTRYRKGMDIAQQQKASFDV
                     YRDKINTQIAYLNQQKELLDSGIQKQLISLKSKMSYLDSTVSQEEAVESFIKERKREL
                     IDKSVQVLGRNKHLQKITKESFYYVETIRNYKELFSDPSKAEVVAKDLLAKVPAFREF
                     FQRNSMLASLFNRPPGQAMNVSGLQTRSSIQDELLSRIGGSGPNASSFVSQQMASAQA
                     EMNKLKQRVLANGSGSGDADMPNFKPNNQKTKTFGQRLEYGFNVQFNRSNRFVPSAVN
                     VAANIGFKLNDKASTGVGMAYRAGIMTNSKFRLSHEGLGLRSYFDWKLKRQFFISGGY
                     ELNQYPAVQDLLQPGNVSVWQKSALIGLSKKVKSSSKFTKNAKLQLLYDLLHRRGISS
                     GPALIYRIGYEF"
     gene            1881..>2842
                     /locus_tag="PK977_09135"
     CDS             1881..>2842
                     /locus_tag="PK977_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18321.1"
                     /translation="MSFKPENVTEMGMLQKLLMIVICCSILNNATAQGVGDVGTPSLL
                     STGALGASKMENTPVNLFTGIPSISIPIFQFSHHSGIETSVSISYYAGGIRVNETPGI
                     AGLGWNLNAGGVIVRNLRGMPDDFPVYGFLNASSIPDDYNTLASQYYNDSIDAQQDVF
                     QFNINGRSGKFLIGKNNQIVQIPETKLRISYRTSTSSYGFGRIIGFTLTAENGVKYLF
                     NDYDTTKTWSDDISYRHKDSGFVNAWYLTDIVSPFGNDTIHFYYQKVNNEVRETLLNY
                     QKLIGEFDTLEMPKVKIGQAAFQRKLSGIKFPDNRKLSFVYDPNR"
     assembly_gap    2843..2924
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2953..>5013
                     /locus_tag="PK977_09140"
     CDS             2953..>5013
                     /locus_tag="PK977_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18322.1"
                     /translation="MAGIRTYTSKGLNPGYHFDYYSPIFPIDSIYNKRDHWGFYNGAP
                     NDTSKHFPAVPYVFPNGANREPNDSAVAGTLKSITDPAGGVTYYDFECNDVYRPYELI
                     PDYQVFNGMVHFSDTISLHHVYRNVDTFKLSYVSLRKLNNISPISVNAELICRITNFD
                     SSVVFGTHTVNLYDLYQSGEQYLAFSNLPDGQVLFKASLSTGSAITARNMFMKLEWDK
                     EVPNTADRIKSNGIRIRQIRHYDPVTGITDTIKTFRYVMNNGRSSGFLGVKPVYTHPY
                     QVNFDNGSIYMTNSYTALNREPVNHFDFSEGSQVSYGRVEVIRGSIYNNLGKEVHEFT
                     TLADFQDYVKPVSFPYVPQFQRDWLLALPKKVSIYNKDGRLIQTTTNEFNYRTSSYDN
                     QYFRSLKLGKLSSTYSGYEIIPANLLSESFEGRFYYPETGRADIKKTTEVFYYEDQST
                     RQNVQEIFYDDQFNVTRLLSDYDKSRNLKIEKRIYYPYNYTLVGPVGRLRDSLIFASI
                     SSEDWIFGDGNPRLIGIQISEFQELPQGYIKPRSSYALRSNKPIPLSQIGEFNPAVLV
                     RNSTLIKEASRNTIFDEKGNLVEVLNPQTGISNVVIMGYSGNLPVATVTQALKSDVAY
                     SSFEASDHGGWELGSAVDFVYEGITGKRAYSLAEGEIVKSGLNSGKTYILTYWKHGSG
                     TVSVS"
     assembly_gap    5014..5060
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5061..5521
                     /locus_tag="PK977_09145"
     CDS             <5061..5521
                     /locus_tag="PK977_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18323.1"
                     /translation="NGWTLVMHKMSQSSTVTISGNATIDELRLYPEESNMTTKTYEPM
                     LGVTSECDANNSIVYQEYDSLLRIKIVRDKDKNILKKHEYPDVITPRNMQNPAKLNYG
                     AFELNPTTGRYDWKCIAQWAYPDGTMSEAFLDPDQQVCPPKPNVTWLPVE"
     gene            5556..>8753
                     /locus_tag="PK977_09150"
     CDS             5556..>8753
                     /locus_tag="PK977_09150"
                     /inference="COORDINATES: protein motif:HMM:NF039454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HRF18324.1"
                     /translation="MKKSLTLYKLLLILCFIISSAINTIAQTLATPEETAGGLRITSE
                     VIERSIARRDSIKNNSIEELELPEEEEETEGYIISGPTMVSRNSIQSYFVRPYGENDM
                     YWYVSGEAVVLDYYDELIQVKFDGEDSVYFIKLFSKDDVLLAEYVVHIYTNSDNFSPG
                     ELITGDQSFLSNQSYSHIDVSEPSGGNCNGNYQYLWQRSYDREIFENINGSTSESLNL
                     SSFVTDMIEHEILTQFFRRRVVCGIDTLFSGIAGVTILPDLQAGVINSTIRYYTIGQS
                     NESIKPEPATGGNCLQNYSYQWQYSYNNTTFTNITGATNDSLIIDNSLDQNIYFRRRV
                     QCGEQVAFTNSVQVMLTEPTLRPLAANTGNQSPVINARKLPPSYASQPISFLRVLAPT
                     IPIQDSAAVNITLPAEHLAVSTTYQDSYGRAIQSVTRQQSPDKKDLVSAATFDAFGRP
                     VVQYMPFVAVSGNRDDGGFKQNAFEQDSIFYRQLFPHEKVIYAEKQYDESPLNIPVRE
                     LAPGNSWGGSGRGVEYFFRANDHEDSVRRWTIAINNENDVPQSTGYYQPGILQVTSVK
                     DESRNWVMKYTDDQGRTVMTKTLLRNRIKYGAKGWLVTYYVYDEMGLLRAVIPPKASE
                     LLCSSAANWNVNYLNTFENLCFAYFYDDWGRAIMKRIPGKGKTYIAYDLLGRVVMTQD
                     AKLRTTQQWAFVKYDNMSRPVKSGLITSSLTKDQIISQASASNDYPSLSGDFTVTSET
                     FYDNYDWISAQSAPFASTIDSSDVTSERFITTYNTSPDYAQPVRSSKRIRGMATGTKT
                     RILHSNTFLFAVNYYDRNGRSVQTQQTNITGGKDVSTVQYAYSGLVLRTHLRHQKQGN
                     NSKEYSNLTKYEYDHAGRLMKLTKNFNSLGDKVITTNSYNELGMLTQKKLGDEMEKQE
                     FDFNIRGWITGINKDFVTSSSAPLPGTIGARYFGEMISYDSGYARALYNGNISGIRWK
                     AVGDRIARSYGFRYDNAGRLMYADFSQQNEGSTAWTKDKVDYTVSGLDYDANGNILSM
                     SQRALKIGASAIVDSLRYEYFSNSNLLRKVTDLVAD"
     assembly_gap    8754..8950
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9271..9289
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9290..>9611
                     /locus_tag="PK977_09155"
     CDS             <9290..>9611
                     /locus_tag="PK977_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18325.1"
                     /translation="PINTVNNKPAGFDQEAENTKVSRLNASPGTNKKTGPSLVLKVMA
                     GDRVQINTWSFYNTAAQQPSSGTNLATELLSILPGAIIGNSGGKLEAANISSVGGALS
                     PNLL"
BASE COUNT         2794 a         1848 c         2082 g         2542 t
ORIGIN      
        1 ggttcgatcc cgccccacac cacagaaaaa caagacaaaa gcctgtaaat caaacgatta
       61 caggcttttt tgtttgtgat cggagcggat ttgggagcgg attatatggt ttattcaact
      121 ctgtcttcat aagcttgatt agatgaaaag cgactatcat catgttgtgc cacttacttt
      181 attctgacgg cttctttttc aatgcagccg gggttaaccc aatcggaaac tatcatttgg
      241 tatatcaggt gagtagattg tgattttttc gttaacagat aaattctttc gtacaataat
      301 ttacggtaca ctaatttcat actaacagat tcccttccgt cccccaacgt taaaaccccg
      361 ctcgccgagg agaaaaacac ctaagatttt ccgttaatta acctgaacgg cacctgtttt
      421 ctttatccat ctttgattta attaataacc aatgtgctat atgtcatcca tacggctgac
      481 catattgctg actttgtgtt tctttcaact tggattgaag gggcaaatag actcatcctc
      541 ttatttgatt tctcagattc catcaagata ttttgaaacg gtagataaaa agacatcaaa
      601 gtacacctcc agaattcaga aaaggacaga gaaaacgctt tccaaactag ccagatggga
      661 gcggaaggtt aagggtatgc tggaaagagc caatccttca atcgcggctg atttgtttgc
      721 tccgggaaga atgacctttg aagacctgta tacgaggtac agaaaaggga tggatattgc
      781 ccagcagcaa aaggcaagtt ttgatgtgta ccgtgataag ataaatacac agattgcata
      841 tctcaaccag cagaaagaac ttctagactc cggtatacaa aagcagttga tttccttgaa
      901 atcaaaaatg agctatctgg acagcacagt aagccaggaa gaggctgtcg aatcattcat
      961 aaaggaaagg aagcgtgaat taattgataa atctgtgcag gttcttggga ggaataagca
     1021 cttgcagaag attacaaaag agtcttttta ctatgttgag acaatcagaa actacaagga
     1081 attgttctct gatccttcta aagcggaagt cgttgctaaa gacctgttag caaaagttcc
     1141 tgcatttcgg gagtttttcc aacgaaacag catgctcgcg tcattgttta accgtcctcc
     1201 cggccaggcc atgaatgtaa gcggtctgca aacacgctca tccattcagg acgagctact
     1261 gtccaggatt ggcggctccg gacccaacgc ctcttcgttt gtctctcagc aaatggccag
     1321 tgcacaagct gaaatgaata agctgaaaca aagggttttg gcaaatggct caggttccgg
     1381 cgatgccgat atgcctaatt tcaaaccaaa taatcaaaag acaaagacct tcggacaaag
     1441 gcttgagtat ggatttaatg tccagtttaa cagatcaaat cgttttgtgc cttcggcagt
     1501 taatgttgct gcaaacatcg ggtttaaact aaatgacaag gcttcaactg gtgtcggcat
     1561 ggcatacagg gcagggataa tgaccaacag taaattccgt ttgagtcatg agggacttgg
     1621 actcagaagt tattttgact ggaaactcaa gaggcagttt tttattagtg gagggtatga
     1681 attaaaccag tatccagctg tacaggattt gctgcagcca gggaacgtaa gtgtttggca
     1741 aaaatctgca ttgattggtc tttccaagaa agtgaaatcc tcttcaaaat tcaccaaaaa
     1801 tgcaaaactc cagttactat atgatttgct gcacaggcgt gggatttcgt ccgggccggc
     1861 actgatttat cgtattggat atgagtttta aacctgaaaa tgttactgaa atgggtatgc
     1921 tacagaaact cttaatgatt gtaatttgct gttccatcct taacaacgcc actgctcagg
     1981 gagtaggcga tgtcggaacg ccgtctctgc tgagcactgg tgctttgggt gcgtcaaaaa
     2041 tggagaatac gcctgtgaat ctgttcacag ggattccatc tatttccatt ccgatattcc
     2101 agttttcaca tcatagcggc atcgaaacca gcgtttccat cagctattat gccggcggta
     2161 tccgggttaa cgaaacccca ggtatcgcgg gtttaggctg gaacctgaat gccgggggag
     2221 tcatagtgag aaacttaagg ggtatgccgg atgactttcc ggtctatggc ttccttaacg
     2281 cgtcgtcaat acccgatgat tacaacacac ttgcatctca gtattataat gacagcatcg
     2341 atgcccagca ggatgtattt cagtttaaca taaacggtcg ctctggaaag tttcttatag
     2401 gaaagaataa ccagattgtt cagataccgg aaaccaagtt gcgcataagt taccgcacat
     2461 ctacttcgtc atatggtttt ggtagaatta ttggctttac cctaactgct gaaaatgggg
     2521 ttaaatactt atttaatgac tatgatacaa ccaagacctg gagcgatgat atcagttacc
     2581 gtcataaaga ttcaggcttt gtgaacgcct ggtatctgac agacattgta tctccgtttg
     2641 gcaacgatac aattcatttt tactatcaga aggtgaataa tgaagttcgg gaaacattgc
     2701 tcaattatca aaagctaatc ggtgaatttg atacacttga aatgccaaaa gtcaagattg
     2761 gccaggcggc atttcaacgg aaattgtcag gaatcaaatt tcctgataat cgaaaattga
     2821 gttttgttta cgacccaaat cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaatg gaggccgctc
     2941 tcaaccgctg ttttggcagg tatcagaaca tatacttcaa aaggcctgaa tcccggttat
     3001 catttcgatt attactcacc aatatttccc atagacagca tctacaataa acgagaccat
     3061 tgggggtttt ataatggggc gcctaacgat acatctaagc attttcctgc tgttccttac
     3121 gttttcccta acggcgcgaa cagagaaccc aacgacagtg cagttgccgg tacccttaaa
     3181 tcaattacag atccggctgg gggtgttaca tattatgact ttgaatgtaa tgatgtttac
     3241 cgtccgtatg aattaattcc ggattatcag gtgtttaatg gcatggtaca tttttcagat
     3301 accatctctt tacatcatgt ttatagaaat gtggatacat tcaaattgtc atatgtatca
     3361 cttcgcaagc tgaataatat atcacctatt tctgtcaacg ctgaacttat ttgccggatc
     3421 accaattttg acagctcagt tgttttcgga actcacacgg ttaatctata tgatctctat
     3481 caatctggcg aacagtatct ggccttttct aacctacccg atggccaggt attgtttaaa
     3541 gctagtttat caacggggtc tgctattact gcaagaaata tgtttatgaa attggaatgg
     3601 gataaggaag tgccaaatac agccgacaga atcaaatcta atggcattcg aatcaggcag
     3661 attcggcatt atgatccggt tacaggaata acagatacaa tcaagacatt caggtacgtc
     3721 atgaataatg gtcgtagttc tggttttctt ggggtaaagc ctgtttacac gcatccatat
     3781 caagtgaatt ttgataatgg gtctatttat atgacaaata gctacacggc attgaataga
     3841 gagccggtca atcattttga cttttcagag ggaagccagg tttcatatgg aagagtggaa
     3901 gttattcgcg gaagcattta caataatctt ggaaaagaag tccatgaatt cacgacactg
     3961 gcggattttc aggattatgt taagcctgtc agtttcccat atgtaccaca gtttcaacgc
     4021 gactggctgc tggcattgcc taaaaaagtc agtatttaca ataaagatgg acgtttgata
     4081 caaacaacaa caaatgagtt taactatcgc acatcgagct atgacaatca atatttccgg
     4141 tcattgaagt taggtaagct ttcttcaacc tattcagggt atgaaataat acctgcaaac
     4201 cttttatcag agagttttga aggaaggttc tattatcctg agacgggcag ggctgatatc
     4261 aaaaagacaa ctgaagtttt ttactatgag gatcaatcaa ccaggcaaaa tgtacaagag
     4321 atcttctatg acgaccagtt taatgtgacc aggttactgt cggattatga caaaagtcga
     4381 aacttaaaaa ttgagaagag aatctattat ccttacaatt atacccttgt tggtccagtt
     4441 ggaagactaa gggacagcct aatttttgca tcaatctcat ctgaagattg gatatttggc
     4501 gatggaaatc ccaggttgat tggcattcag atttcggaat tccaggagtt gccacaggga
     4561 tatattaaac cgcgttcttc ctacgcatta agatcaaata agcctattcc tctttctcaa
     4621 atcggcgagt ttaatccggc tgtcctggta agaaacagca cattgatcaa agaagccagc
     4681 aggaacacga tattcgacga gaagggtaac ctggtagaag tgcttaaccc acaaacaggc
     4741 atctctaatg tagtcatcat gggatatagt ggaaatttgc cggtggctac cgtaacacag
     4801 gctttgaaaa gcgatgtagc atacagcagt tttgaggcct ctgaccacgg aggctgggaa
     4861 ctcggttctg ctgtggattt tgtttacgaa ggcattacag ggaaaagagc gtattcctta
     4921 gccgaaggag agattgtcaa atcaggcctt aacagtggaa agacgtatat cctgacttat
     4981 tggaagcatg gatctggaac ggtgtctgtt tcannnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn aaaacggttg gaccctggtc atgcataaga tgtcacagtc
     5101 ttcaactgtt acaatttcag gaaacgccac catagatgaa ctcaggcttt atcccgaaga
     5161 gtctaatatg actacaaaga cctatgagcc tatgctggga gtgaccagtg agtgcgatgc
     5221 caacaatagc attgtatatc aagagtatga tagtttactg cgaattaaga tcgtgaggga
     5281 taaggataag aacattctga aaaagcatga atatccggat gtaatcactc ccagaaatat
     5341 gcagaatcca gccaagttga attatggtgc ttttgaattg aatccaacta caggcagata
     5401 tgattggaaa tgtattgcac agtgggcgta tccggatggc acaatgagtg aagcattttt
     5461 ggatcccgac caacaagttt gtccgccaaa gccgaatgtt acctggctgc ccgttgagta
     5521 aaaaccccat tactatctgt ttaaatcatg aatacatgaa aaagtcactc acattgtata
     5581 agttattatt gatactgtgt ttcattattt cctctgcaat aaacaccatc gcacaaaccc
     5641 tagctactcc cgaagaaaca gctggtggat taagaattac atcggaagtt attgagcgtt
     5701 caattgccag aagggattca attaagaata actcaataga ggaacttgag cttcctgaag
     5761 aggaagaaga aacagagggt tatataatat ccgggccaac catggtttca agaaattcaa
     5821 tacaatcata ttttgtaaga ccatacggag agaatgatat gtattggtat gtgagtggag
     5881 aagctgtagt tttagattac tatgatgaat taattcaggt taaatttgac ggggaagact
     5941 cagtttactt tattaaatta ttttcaaaag atgatgtctt gcttgcggaa tatgtagttc
     6001 acatttatac aaattcagat aatttttcac caggagaact aataaccgga gatcagtcgt
     6061 ttttatcaaa tcagtcatat tcacatatcg atgtttctga gccttcaggt ggtaattgca
     6121 atggtaatta tcagtatttg tggcaaagaa gttatgatag agagattttt gagaatatca
     6181 atggatcaac atctgaatcc ctcaatttga gcagctttgt aacggacatg atcgaacatg
     6241 aaatcctaac tcagtttttc agaagaaggg ttgtatgtgg gatcgatact ttattttccg
     6301 gaattgctgg agttaccata ctacccgatt tacaagcagg tgtgattaat tcaaccattc
     6361 ggtattatac tattggtcaa agtaatgaat cgatcaaacc agaaccagct accgggggca
     6421 attgtttaca gaattatagt tatcaatggc aatattctta caataacaca acgtttacca
     6481 atattaccgg cgccacaaat gattctttaa tcatcgacaa ttctcttgat cagaacattt
     6541 atttccgcag aagggttcaa tgtggtgagc aggttgcttt taccaacagt gttcaagtaa
     6601 tgttaacaga gccaactctc cgccctttag ccgctaatac gggaaaccaa tcaccggtta
     6661 ttaatgccag gaagttacct ccatcttatg ccagtcaacc catcagtttt ttaagggtac
     6721 ttgcaccgac cattcccata caggatagtg ccgcggtaaa tatcacccta ccggcagaac
     6781 acctggcggt atccaccact taccaggatt catatggacg ggcgattcaa tcggttaccc
     6841 ggcaacaatc tccggataaa aaagaccttg tatctgccgc tacctttgat gcctttggaa
     6901 ggccggttgt ccagtatatg ccttttgtgg ctgtttcagg aaatagggat gacggtggat
     6961 ttaagcaaaa cgcttttgaa caggactcca ttttttaccg ccagctattc ccacacgaga
     7021 aagtgattta tgccgaaaag cagtatgatg aatcaccctt gaatattccg gtacgcgaac
     7081 ttgcccccgg caatagttgg ggaggctcag gaaggggtgt agaatatttc tttagggcaa
     7141 acgatcacga agacagtgtg cgcaggtgga caatagctat caataatgag aatgatgtgc
     7201 cccaatcaac agggtattat cagcccggta tcttgcaggt taccagtgta aaggatgaga
     7261 gcagaaactg ggtaatgaaa tacaccgatg accagggcag aactgttatg actaagacat
     7321 tattgcgtaa caggatcaag tatggtgcca agggctggct tgttacttat tacgtgtatg
     7381 atgaaatggg cctgcttagg gcagtgattc caccgaaggc ttctgaattg ctttgttctt
     7441 cggctgccaa ttggaacgta aattacctta acacattcga gaacctgtgt tttgcttatt
     7501 tctatgatga ctggggccgg gcaatcatga agcgcattcc cggaaagggc aaaacctata
     7561 ttgcgtatga tctgctgggc agggtggtaa tgacacagga tgctaaactg aggactacac
     7621 agcaatgggc ttttgtcaag tatgataata tgtcaagacc ggttaagtct ggtttgatta
     7681 cttcttcact taccaaagac cagatcatca gccaggcatc ggccagcaac gattatccat
     7741 ctttaagtgg cgacttcacc gttacttcag agactttcta cgataattat gactggattt
     7801 cggcccagtc tgctcctttt gcctcaacga ttgatagttc agatgtgacg tcagagcgat
     7861 ttatcacgac gtacaataca tccccagatt atgcacagcc agtcaggagt tccaaaagga
     7921 taaggggtat ggcgactggt accaagacaa gaatactaca tagcaataca ttcctgtttg
     7981 ctgtaaacta ctacgacagg aacggaaggt ctgttcagac ccagcaaacc aatattacag
     8041 gcggaaagga tgtatctact gttcaatatg cttacagcgg gctcgtatta cgaactcacc
     8101 tgcggcacca gaaacaagga aataattcga aggagtatag taacctgacc aagtatgagt
     8161 atgatcatgc gggcaggctg atgaaactga ccaagaactt taactccctg ggcgataaag
     8221 tcatcaccac aaacagctat aacgaattgg gcatgttgac ccagaagaag ctgggtgatg
     8281 agatggagaa acaggaattt gactttaata tcaggggttg gataaccggt atcaataaag
     8341 attttgtgac cagtagttca gcgccgttac ccggaacaat cggtgccagg tattttgggg
     8401 aaatgatctc gtatgattcc ggatatgcca gggccctgta caatggcaat atttcaggga
     8461 tacggtggaa ggcagttgga gatcgtattg cccggtccta tggattcaga tacgacaatg
     8521 caggccggct gatgtatgcg gatttcagcc agcagaatga aggcagcacc gcctggacca
     8581 aagacaaagt ggattatact gtatcaggac ttgattatga tgccaatggt aacatcctgt
     8641 caatgagcca gcgggctttg aaaatcggtg cttccgcgat tgtagactca ttgcgctatg
     8701 agtatttctc aaacagcaac ctgctaagaa aggtaactga ccttgtagca gacnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn gatgctgcgg gtaatctgct taggaagaag atttcagtac aggaaggagg
     9001 acaaacagtg cagaaggagt acgtatacgt ggcgggattt gtttatttaa atgatacttt
     9061 acagtattcc ttgatggaag aaggttgcgt gcggcgtgtc atgcggagaa gtttccagac
     9121 gggtgagatg tatgaggctt ttgattacca gtattatgta aaagaccggc agggaaatat
     9181 cagggcggtc ttaacggagg gcaaagacac atctttctac caggcgacca tggaaccggc
     9241 caatgaaaca ctggagaatg ccctgttttc nnnnnnnnnn nnnnnnnnna cccgattaat
     9301 acggtcaaca ataaaccagc cgggtttgac caggaagccg agaacacaaa ggtttcaagg
     9361 ctaaatgcat caccaggaac caataagaaa accggtccat cgctggtgct gaaagttatg
     9421 gcgggcgaca gggtgcaaat caacacctgg tctttttaca atacggcggc acaacagcct
     9481 tcatccggta ccaatctggc aacggagctg ttgtctattc ttcccggtgc cattattggt
     9541 aacagtggag gaaagctgga agcggcaaat atctcgtccg ttggtggggc tctgagtccg
     9601 aatttattgc a
//
LOCUS       DAORLS010000103         9568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3843_length_9568_cov_2.275833, whole genome shotgun sequence.
ACCESSION   DAORLS010000103 DAORLS010000000
VERSION     DAORLS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_9568_cov_2.275833"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK977_09160"
     CDS             complement(<1..448)
                     /locus_tag="PK977_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRF18326.1"
                     /translation="MRKLIIPISAGMMCLSFTIIPPKSQQPASAATITTSSVSDAVSA
                     KEMASQLYNDICLQEYGLSKTAFEYAWKGYQYLVEKGSIKRSEYLTICDFSQSSRQKR
                     LYIIDVANAKLVTNTWVAHGRNSGNEYATKFSNIPESLQSSLGFYVT"
     gene            complement(657..1703)
                     /locus_tag="PK977_09165"
     CDS             complement(657..1703)
                     /locus_tag="PK977_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRF18327.1"
                     /translation="MSNNVIDQNRVRQFFFIVLILLLGILLSFQLYSFLPAMLGAITL
                     YILMHRWMFYLTETKKWRKGLAALLLMFISFIVILLPVGLLVNMLSSKVSYAIQHSAE
                     LTTALKKVVTDIETQYNIVIASDENINKLGNAIAESLPKILGATFNTLTTIFFMYFIL
                     YFMLVNARRMEDALYEYIPLRDENVSKIGKEIHTMVYSNAIGIPLIAFVQGVVGLIGY
                     LIIGVKEPFFWFGVTCIAAMLPVVGAALAYVPLSIIFFANGQSGQGIALLIFGFGIIG
                     LVDNVLRFTLLKRMANVHQLVTVFGVIIGLNLFGFLGLIFGPLLISMFILLLKIYTTE
                     FVTKQRDIHKIMEN"
     gene            complement(1783..3204)
                     /locus_tag="PK977_09170"
     CDS             complement(1783..3204)
                     /locus_tag="PK977_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRF18328.1"
                     /translation="MSRQTKTNKLTYFFSLVRQSLKGGEHDYTQGSIRGAIVLLAIPM
                     ILELSLESVFAVVDMYFVSHLPDNEIAIATVGLTESVIAIIYTLAIGLSTGATAVVAR
                     RIGEKNPEAAAHAGAQSILLAVAAAVLLSITGAIFAPEILRLMKAEERVVQEGAVFTR
                     IMLGGSTVIVLLFLINGIFRGAGDAAIAMRSLWLASIINIFLCPMLINGYGPFPELGL
                     KGAAIATTIGRGAGVVYQCYYLFHSKGNIRIKWPHFKWDFPVIKHVYEVAWPATLQFF
                     IQSGSWIVLAWLVSDTGSTNASAGYQVAIRNVVFFILPAWGLSNAAATLVGQNLGAKQ
                     PDRAEKSVLLTTKYNALFMAGVMLLFLLFASPIISIFTNNPEVHAYGVLSLQIIGSGY
                     IFYGIGMVMIQALNGAGDTRTPTWINFFGFWLFQIPFAFLLAKVFKLGPLGAFIAIPV
                     AETVMALVAYYYFRKGKWKQVAV"
     assembly_gap    3470..3497
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3498..>3852)
                     /locus_tag="PK977_09175"
     CDS             complement(<3498..>3852)
                     /locus_tag="PK977_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRF18329.1"
                     /translation="LLDKFGDSIRFLRDPTRGGPASVLNEIAGLTRLGYQLHQSALPI
                     DPQVEGACEMLGLDPLYIANEGVFLAIVKADVADEFLSALQQDSNGIHAAIIGEVTNT
                     NTGKVILNSSIGGQRV"
     assembly_gap    3853..3877
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3878..>4161)
                     /locus_tag="PK977_09180"
     CDS             complement(<3878..>4161)
                     /locus_tag="PK977_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRF18330.1"
                     /translation="GLTMEEFWTILVSIKEAANKADVKIVTGDTKVVEKGKGDKVFLN
                     TSGIGIMHPKAKIHHNNIQTGDKIIISGQLANHGITIMSLRKGLEFETTI"
     assembly_gap    4162..4228
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4229..4477)
                     /locus_tag="PK977_09185"
     CDS             complement(<4229..4477)
                     /locus_tag="PK977_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRF18331.1"
                     /translation="MMQLNCPMPKFDFDVITLGHGSGGLLTHKLLESGVFDLFKNDLL
                     DQQHDGASFSLSGDVSFSTDSYVVTPIFFPGGNIGDLAV"
     gene            complement(4487..6733)
                     /locus_tag="PK977_09190"
     CDS             complement(4487..6733)
                     /locus_tag="PK977_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF18332.1"
                     /translation="MKKGILFFTMLVFNTSFIFSQSLPKGISIYNQNSKTPIPDVSVY
                     TSDFNFAEVSDENGFVSLKNLPASSKSIIISAIGFETKNILLSEITNDIIWLLPKITT
                     LTDVVVRASAKTGIFKTISDLDIHLRPIINSQEVLRMVPGLFIGQHAGGGKAEQIFLR
                     GFDLDHGTDIHLTVDGLPVNMVSHAHGQGYADLHFVIPELIEKVNFSKGPYYSDKGNL
                     ATAGYVAFKTKNYLEKNFFKLEGGQYNTVRAITGINLLPKGSSEKKQSLYIAGEGSFT
                     KGYFDSPQNFSRFNGMIKYHGKTGDNSTLTALLSGLTSKWNASGQIPDRAVADGTIGF
                     FGAIDDNEGGNTNRYNASAELMHNLSNGAVLKNQLFYSRYLFELYSNFTFFKEDPVNG
                     DQIRQKEKRNILGYNLSYQRDNSIGKKKGQFTAGTQLRYDDVNDIELTRTKNRNVNTR
                     DIMLGDINEFNAGFFTQQKVVLSSQWDIATGVRLDYFTNKYNDKLAATVQSSNSVIVS
                     PKLNINYRMNDKVQLYWYSGQGFHSNDTRVAVVQNGREVVTPAWGSDIGGIFKLGRKA
                     MLQTALWYLWMKQEFVYVGDEGVVEPGGKTQRIGWDLSLRYELSKNLYADADFTVTNP
                     RALEVDKAESYLPLAPRFTSVGGITYRKEKGWNGSLRYRYMANRPANEDNSVVAKGYF
                     VTDAAINYTKKNWEAGISIQNLFNTKWKETQFDTESRLQNETDPVSEIHFTPGTPFFA
                     RLSFSLFF"
     gene            complement(6812..>7862)
                     /gene="hypD"
                     /locus_tag="PK977_09195"
     CDS             complement(6812..>7862)
                     /gene="hypD"
                     /locus_tag="PK977_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRF18333.1"
                     /translation="QYFEEIRRVTTKPWTLMEICGGQTHSLVRNGILDLLPDKITMVH
                     GPGCPVCVTSISVIDEAIHLAQMENVILCSFGDMLRVPGSSKSLLEVKSAGADVRILY
                     SPLEAVQLAQQSPEKEVVFFAVGFETTAPANALSVIQADKLGVTNYSILASHVLVPPA
                     MEALLSDPENKIDGFLAAGHVCTIMGMEEYYPIAEKYKTPIVVTGFEPLDLLQGILMT
                     VMQLEKGEYKVENQYARSVQREGNRLAKDSIVKVFTIGDRQWRGIGSIPQSGYEVNER
                     YKKYNARLKFDLNIPLAEENKACISGDIMKGLKKPVQCPNFGTLCKPEHPLGAPMVSS
                     EGACAAYYHYSDKNY"
     assembly_gap    7863..7878
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7911..8162)
                     /locus_tag="PK977_09200"
     CDS             complement(7911..8162)
                     /locus_tag="PK977_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRF18334.1"
                     /translation="MCLAIPGKILSIIPKTDETFRMGKVSFGGITKEVNLSMVPDAAV
                     NDYVLVHVGVAISRIDEEEAKLTFEYLKQIGETNELNDE"
     gene            complement(8177..>9568)
                     /locus_tag="PK977_09205"
     CDS             complement(8177..>9568)
                     /locus_tag="PK977_09205"
                     /inference="COORDINATES: protein motif:HMM:NF036810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRF18335.1"
                     /translation="PCAPLLQLLVNDFGKPLIATSANISGSPIIYNDEEALEKLSGIA
                     DYIITYEREIVVPQDDSVLQLTDKGEKIILRRSRGMAPNYFPNPFEATDECILATGGE
                     LKNAFAIQERHRLYISQFLGNQESLASQEAFTHTSNHLFDLLQTKPSVILADKHPGYF
                     VSQRANELSLENNIPLFSVQHHEAHFGAVLAENGLLSAQEPVLGIIWDGAGYGDDQQV
                     WGGEFFVFEYGEINTTVHFDYFPQLLGDKMSREPRLSALSLLKQLPAKQSVLEPYFSV
                     TEWQYYQQLIKQPATLLTSSVGRLLDAVACILGICSINTYEGEAAMKLEAVARSYRGE
                     MTDYYPLPVKNNRIELLLMLQDMMHDREAGMPVNQVAAKIFYSLALTVGRMSDHLGID
                     KIAFSGGVFQNALLTNMIDSLYAGKKQLYRHKQLSPNDECIGFGQLACYHLSKKRQME
                     MQLPASMIISEMN"
BASE COUNT         2714 a         2191 c         1829 g         2698 t
ORIGIN      
        1 aagtaacata aaagccaaga ctgctttgaa gggattctgg tatgttggaa aacttggtgg
       61 catattcatt gccgctgttc ctgccatgtg ccacccaggt attggtaacc agtttagcat
      121 tggcaacatc gattatatac aatctctttt gacgggaaga ctgactaaaa tcgcagatag
      181 ttaaatactc agatcttttg atgctaccct tctctaccag gtattgatat cctttccatg
      241 catattcaaa agctgttttt gaaagaccat attcctgcag gcaaatatca ttgtataact
      301 gacttgccat ttccttcgca gaaaccgcat cactcacaga agaggtagtg atagtcgcag
      361 ccgatgcagg ctgctgtgat tttggtggaa tgatggtaaa tgacaggcac atcattcctg
      421 cggatattgg aataataagt tttctcactc aggtacggtt tttctctgaa cacccttttg
      481 ggtcttgttg ttcagactgg gttctttaaa cggaagccga tgctaaaata ttattcaatc
      541 attaaaaatt ctaacaataa ctacttgttt ttgatgtatt tgcgaagaag tacgctgagt
      601 gaagtaaata tttgcaaggg aaaatacgag tgcataaata gatatactta gctgaattag
      661 ttctccatta tcttatgtat atctctttgc ttggttacaa attcagtggt atatatttta
      721 agcaacaata taaacattga aattagcaac ggaccaaaaa tcaatcctaa aaagccaaac
      781 aggttcaacc cgataatgac accgaataca gtgaccagct ggtgtacatt ggccatcctc
      841 tttaataagg taaagcgaag cacattgtca accaggccaa tgataccaaa tccaaagatc
      901 aacaaggcaa ttccctgccc cgattggccg ttggcaaaaa atataatcga aaggggaaca
      961 taggccaatg cagccccgac caccggcaac atagcagcaa tacaggtgac gccaaaccag
     1021 aaaaaaggct ccttcactcc aatgataaga taaccgatca atcccaccac cccttgcaca
     1081 aaagcgatca gcggtatacc aatggcattg gaataaacca tcgtatgaat ttctttacca
     1141 atcttggaca cattttcatc tctcaacgga atatattcat acaacgcatc ctccatcctg
     1201 cgggcattca ccagcataaa atatagaatg aaatacatga agaatatggt cgtaagtgta
     1261 ttaaatgtgg cccctaatat tttaggaagg ctttctgcaa tagcattgcc cagcttattt
     1321 atattttcat cactggctat aacaatatta tattgtgttt cgatatctgt taccactttc
     1381 ttcaatgcag ttgtaagttc tgctgaatgt tgaatggcgt aactaacctt cgaagaaagc
     1441 atgtttacca gcagtcccac aggtagcaga atcacaataa aggaaataaa catcagcagt
     1501 aaagcagcaa ggcccttcct ccatttcttt gtctcggtca ggtaaaacat ccaccggtgc
     1561 atgagaatat acagcgttat agcgccaagc attgccggta aaaaagaata caactgaaac
     1621 gagagcagga taccgagtaa taatatcagc actataaaga aaaactggcg gaccctgttc
     1681 tgatcaatta cattattact catataccaa aaataagaag taggaaatac gaagtatgga
     1741 atgcagacca aagactcatt ccacacttgg taaaaatatt gattaaacag cgacctgttt
     1801 ccattttcct ttcctaaagt aatagtaagc cacaagagcc atgaccgttt ctgctacagg
     1861 aatagcaatg aaagcaccca gggggcccag tttaaatacc ttggccagca ggaaagcaaa
     1921 cggtatctgg aacaaccaga agccaaaaaa attgatccag gtcggcgtac gggtatcgcc
     1981 ggctccattt agtgcctgta tcatcaccat tccgattccg taaaaaatat agccgctgcc
     2041 aatgatctgc aatgacaaaa ccccataggc atgcacttcc gggttattcg taaaaatgct
     2101 gatgatgggt gaggcaaaga ggagaaacag caacattaca ccggccataa atagggcatt
     2161 atactttgta gtaagcaaga cacttttttc tgcccggtct ggttgtttcg cacccaggtt
     2221 ttgacctacc agcgtagctg cggcattact caatccccat gcgggcagga taaagaaaac
     2281 cacgttacgg atcgctacct ggtagcctgc actggcattg gtactgcctg tatcagaaac
     2341 cagccaggcc agaacgatcc agctgccgct ttgaataaaa aactgcaggg tagcaggcca
     2401 tgccacttca tatacatgtt tgataactgg aaaatcccat ttaaaatgtg gccattttat
     2461 ccggatattt cccttactgt gaaaaagata atagcattga tacactactc cggctcctct
     2521 tccaatagtg gtagcaatcg ccgcaccttt caatcccagt tccgggaacg gtccatatcc
     2581 attgatcagc atggggcata gaaaaatatt gatgatactg gccagccaca aacttcgcat
     2641 ggcaatggcc gcatcaccgg ccccgcggaa aataccattt atcaaaaaaa gcaaaacgat
     2701 taccgtgctg ccacccagca tgatacgggt aaacacagcg ccttcctgca ctactctttc
     2761 ttctgctttc attaatcgca agatttcggg tgcaaaaatg gcacctgtaa tactcagcaa
     2821 tacagcagca gcaacagcca gtaaaataga ctgtgcgccg gcatgggcag cagcttcagg
     2881 atttttctcg ccaatccgtc gggctaccac tgccgtggcg ccggtgctca ggcctatggc
     2941 cagtgtgtaa atgatagcta ttacagactc agtaaggccg actgttgcaa tagctatttc
     3001 attatcgggt aaatggctga caaagtacat atctactact gcgaatactg attcaagact
     3061 taattcaagt atcatgggta ttgccagcag tacaatggcg ccgcggatac ttccctgcgt
     3121 atagtcatgc tccccaccct ttagtgattg cctgactaat gaaaagaaat aggttagttt
     3181 attggttttt gtttgtctcg acatggttct tggtttgccc aggacatagc acaaactata
     3241 acctggtata tgaaaataaa ttaaatgaaa ttgaaaaaca gcagctaatg ctgttgattg
     3301 acgagcggtc ttattatttc aggttctgtc ccgggatacc cgggattaca atgggatcga
     3361 accttagcag attttcatat tcgttacttg aaaggaatgc gaagataaaa gaaaccggta
     3421 aatggctaat cacataaata tgtgaatgaa aaatatcagc agatacgggn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnncca cccgctgtcc gccaatactg ctgttgagga ttacctttcc
     3541 cgtatttgta ttggttactt ctccgataat ggcggcgtga atgccattac tgtcttgttg
     3601 aagagcagac aaaaattcat cagccacatc agccttaacg atagcgagga atactccttc
     3661 atttgcaatg tacaaaggat caagtcccag catctcacag gccccttcca cctgcggatc
     3721 aatcggcaac gcagactgat gaagctggta tccgagacgg gtaagaccgg caatttcatt
     3781 taacacagaa gccgggcctc ctcttgtcgg gtcacggaga aaccggatac tatcaccaaa
     3841 tttatccagc agnnnnnnnn nnnnnnnnnn nnnnnnntgt atggttgttt caaattccag
     3901 tccctttctc aggctcataa tggtgatgcc atgattagcc agctggccgc tgataatgat
     3961 tttatcgccc gtctgtatat tattatggtg aatctttgct ttagggtgca tgatgccaat
     4021 accagacgta ttaagaaaca ctttatctcc tttacctttt tctactactt tggtatcccc
     4081 tgttacaatt tttacgtccg ccttgttggc tgcctctttt atgcttacca gtattgtcca
     4141 gaattcctcc atcgtcaatc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ctgccaggtc accaatatta ccacccggaa
     4261 agaaaatggg agtaaccaca taactgtctg tactaaatga cacgtcaccg gacaaagaaa
     4321 aagaggcccc gtcatgctgc tgatcaagca ggtcattttt gaataaatca aatacaccgc
     4381 tctccagtaa tttatgcgtt aataacccgc cgctgccatg acctaaagta atgacatcaa
     4441 aatcgaattt gggcatgggg caatttaatt gcatcattta cggggtttaa aaaaacaaac
     4501 taaaactcag tcttgcaaag aaaggtgttc cgggtgtaaa gtgaatctct gatacagggt
     4561 cagtttcatt ttgcaacctg ctttcggtat caaactgtgt ctccttccat tttgtattaa
     4621 agaggttttg tattgaaatg cctgcttccc agtttttctt tgtgtagtta attgcagcat
     4681 cagtaacaaa atatccctta gccactacac tgttgtcttc attggccggt cggttggcca
     4741 tataacggta acgcaagctt ccattccatc ctttttcttt tctgtatgta atgccaccca
     4801 cactggtaaa tcttggagcc agcggcaggt aactttctgc tttgtcaact tccagcgccc
     4861 ttggattcgt gacagtgaag tctgcatctg cataaaggtt tttgcttagt tcgtagcgca
     4921 gcgataaatc ccagccgatg cgttgcgttt tgccaccggg ctccactaca ccttcatcac
     4981 caacgtacac aaactcctgc ttcatccata aataccataa agcagtctga agcatggcct
     5041 tcctgcctaa tttaaaaata ccaccgatat cactgcccca ggcaggagta actacttccc
     5101 ggccattttg gacaacagcc accctggtat cattgctgtg aaaaccctga ccgctgtacc
     5161 agtaaagctg aaccttatca ttcatccggt aattaatatt gagttttgga ctaacaatta
     5221 cagaattgga tgattgaacc gttgcagcaa gtttatcgtt gtatttattc gtgaaataat
     5281 ccagcctgac tccggttgca atatcccatt ggctgctgag tactactttc tgctgtgtaa
     5341 aaaagccggc attaaactca ttgatatcgc ccagcataat atcccgggtg ttgacattcc
     5401 tgttttttgt tcttgttaat tctatatcat taacatcatc atacctgagt tgtgtaccgg
     5461 cggtgaattg ccctttcttc tttccaatac tattatcccg ctggtaggat aaattataac
     5521 caagaatatt tcttttttcc ttttgtctta tctggtcacc attaaccggg tcctctttaa
     5581 aaaaagtgaa attggaatac agttcaaata aatagcggga ataaaaaagc tgattcttta
     5641 aaacagcgcc gtttgataaa ttatgcatca gctcggcaga agcattgtag cggttggtgt
     5701 ttcctccttc gttatcatca atggcgccaa aaaaaccaat agtgccatcc gcaactgcac
     5761 ggtcaggtat ctgaccactg gcattccact tgctggtaag gccgcttagc aatgctgtaa
     5821 gtgtactgtt gtcacccgtt ttgccatggt acttaatcat cccgttgaac cgtgaaaaat
     5881 tctgcggact gtcaaaatac cctttggtaa atgagccctc gccggctata taaagactct
     5941 gctttttctc gctgcttcct ttgggcagca ggttaatgcc agtaatagcc cttacagtgt
     6001 tgtattgacc gccctcaagt ttgaagaaat ttttttccag gtaatttttt gttttgaagg
     6061 ccacatatcc cgcagtggca aggtttccct tgtcggagta ataaggacct ttgctgaagt
     6121 ttactttttc aatcaactcg ggaatcacaa agtgcagatc ggcatatccc tgcccgtgtg
     6181 catggctgac catatttacg ggcagtccat caacggttaa gtgaatatcg gtgccatggt
     6241 caaggtcaaa accacgaaga aaaatttgtt cggctttgcc accgcccgca tgttgcccga
     6301 taaataaacc gggaaccatc cttaatactt cctgtgaatt aataatagga cgcaaatgaa
     6361 tatcaagatc gctgatggtc ttaaatattc ctgtttttgc cgaagccctc accaccacat
     6421 cggttagtgt tgtgattttg ggcagcagcc agatgatgtc attcgttatt tctgacagca
     6481 gaatattttt tgtttcaaaa ccaatggctg atataataat ggattttgag ctggcaggaa
     6541 gatttttcag cgaaacaaaa ccgttttcat cactcacttc agcaaagttg aaatcggatg
     6601 tgtatacaga cacatccgga atgggagttt tgctgttttg attgtaaata ctgattcctt
     6661 ttggcaacga ttgtgagaag atgaaactgg tgttaaaaac caacatggta aaaaataaaa
     6721 ttcctttttt catacaccca tttatttgaa acagtaagaa attaagtttc attaagaatt
     6781 gtttcttcat ttccttaatg ccttaatggt tttaatagtt tttatctgaa taatgataat
     6841 atgctgcaca ggctccttcg ctgctcacca tgggtgcacc cagcggatgc tctggtttgc
     6901 ataaagtgcc aaaattgggg cattgaaccg gctttttcaa acctttcata atatctccgc
     6961 tgatacaggc tttgttttct tcagccaaag gaatattgag atcaaacttc agacgggcat
     7021 tatacttttt ataccgttca ttcacttcat aaccgctttg cggaatggag ccaatgcccc
     7081 tccattgcct gtcaccgatt gtaaaaactt tcacgatcga atcctttgcc agccggttcc
     7141 cttctctttg cactgacctt gcatattgat tttctacctt gtactctccc ttttccagct
     7201 gcatcaccgt catcagtatc ccttgtagta aatccaacgg ttcaaaaccg gtaacaacga
     7261 tgggtgtttt atatttttca gcgatgggat aatactcttc catccccatg atagtacaga
     7321 catgccccgc cgccaaaaac ccgtctattt tattttccgg atcacttaat aaagcttcca
     7381 tggctggagg caccaaaaca tgtgaagcaa gaatgctgta gttggttact ccaagtttat
     7441 ccgcctgtat cacactgagt gcattagccg gagcagtggt ttcaaaacct actgcaaaaa
     7501 aaacaacttc tttctcgggg ctttgttgag ccagctgaac agcttcaaga ggagaatata
     7561 aaatccttac atcagcacct gcagatttta cttcaagtaa actttttgat gaacccggta
     7621 cccgtaacat atctccaaaa gagcagagaa tgacattttc catttgtgcc aaatgaatgg
     7681 cctcatcaat tacactgata ctcgtcacac aaaccgggca gcccggccca tgcaccatgg
     7741 taattttatc cggtaacaga tcaaggattc cgtttctgac gaggctatgc gtttgcccgc
     7801 cacaaatttc catcagcgtc catggcttag tggtaaccct gcgtatctct tcaaaatact
     7861 gcnnnnnnnn nnnnnnnnca gctccggatc cctgtattct gataaaaatt tcattcgtca
     7921 ttcaattcat ttgtttcacc aatctgcttc aggtactcaa atgtcagttt tgcttcttcc
     7981 tcatcaatac ggctgatggc tacccccaca tgcaccagca cataatcatt caccgcagca
     8041 tccggcacca tactaaggtt tacttctttg gtaataccgc caaaagaaac ctttcccata
     8101 cggaaagttt catccgtttt tggaatgatc gataatatct ttcctggtat tgctaaacac
     8161 ataattcaag atacatttaa ttcatttcac taattatcat agatgccggt aattgcattt
     8221 ccatctgtct tttcttcgat aaatgataac atgccagttg tccaaatcct atacactcgt
     8281 cattcggact taattgcttg tggcgataca actgcttctt acctgcatat aagctatcaa
     8341 tcatattggt cagcagtgca ttttgaaaaa caccgccgct gaaggcaatc ttatctatac
     8401 ccaggtgatc actcatcctg ccaactgtta atgccagcga gtaaaatatt ttagcggcaa
     8461 cctggttaac gggcattcct gcttccctat catgcatcat atcctgcaac atcagcaata
     8521 gttcaatacg gttattcttt acaggcaatg gataataatc agtcatttca ccccggtaac
     8581 ttcttgcaac tgcctccagt ttcatggctg cttcgccttc ataagtattg atggagcaga
     8641 ttcccagtat gcaggcaacg gcatcaagca ggcggcccac actgcttgtg agaagtgtgg
     8701 ccggttgttt tatcaattgc tgataatact gccactcggt tactgaaaaa tagggttcaa
     8761 gtacagactg tttggcgggc aactgtttca gcagacttaa tgcagacaat cttggttcac
     8821 ggctcatttt gtcaccgagc aattgcggga aataatcaaa gtgtaccgtc gtgttgatct
     8881 caccatattc aaaaacaaaa aactcgccgc cccatacctg ctgatcatca ccatagccgg
     8941 ctccgtccca aataattccc agcacaggtt cttgtgcaga caacagacca ttctctgcca
     9001 gcacagcccc gaaatgtgct tcgtggtgct gcactgaaaa gagtggaata ttattttcaa
     9061 gcgatagttc atttgcccgc tgcgaaacaa aatagcccgg gtgtttatct gcaagaataa
     9121 cagaaggctt agtttgcaac aaatcaaata aatgattact ggtatgcgta aaggcctcct
     9181 gtgatgcaag gctttcctgg tttcccaaaa actggcttat ataaagccgg tgccgttcct
     9241 gaatggcaaa agcatttttc aattcaccgc cagtagccaa tatgcactca tcagttgctt
     9301 caaaagggtt cggaaaataa ttaggcgcca ttccacggct tctccgtaaa ataattttct
     9361 ctcctttatc agtcagttgc aatacgctgt catcctgcgg cacaacaatt tctctttcat
     9421 atgtgatgat ataatcagct atgccggaaa gtttttcgag tgcctcttca tcattataga
     9481 tgattggcga accacttata ttggcactgg tggcgatcaa tggttttcca aaatcattaa
     9541 ccagcagttg caacagcggt gcacaagg
//
LOCUS       DAORLS010000104         9513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3875_length_9513_cov_2.230916, whole genome shotgun sequence.
ACCESSION   DAORLS010000104 DAORLS010000000
VERSION     DAORLS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_9513_cov_2.230916"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PK977_09210"
     CDS             complement(<1..578)
                     /locus_tag="PK977_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18336.1"
                     /translation="MTKRIKAILVLLLCSLFLNAQNIKLVSGMKISRSGKVLKKEYKL
                     DGLSDNDKPVVLIEGENITIDFNQAVLQGSNQKLRPDEFFGVAILIRNGRNITIKNLV
                     ARGYKVALLAKNVEKLVLENCDFSYNYRQHLNSTQEKEDISDWMSYHHNENDEWLRYG
                     AAMYLRNCINTIVKNCKVTGGQNALMMMECNGG"
     gene            613..1500
                     /locus_tag="PK977_09215"
     CDS             613..1500
                     /locus_tag="PK977_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRF18337.1"
                     /translation="MQEQSKQPSRVSKWLKLLLKIAVMTVCLWYVSGKIDFERSKTVL
                     QHSNWLILFIALLLFVVSKFVSAIRLNIYFKNIGIILSEVENIRLYLLGMFYNLFLPG
                     SVSGDAYKVVLLTKRYSIPYKKTTAAVLLDRFSGLLGLGILLTTCSYIVLNFIYCLIL
                     TAITVGAIIGFYYIIKRWFKDFMSGFWPTLLLGILVQAVQVICIYMIIQSLGVEKNNA
                     VYIFIFLVSSAVSVLPLTIGGLGIREVVFLEGAAYFSLDNETSVIISLLFYLITLVVS
                     ATGLGYIFKDSLKKKERTQ"
     gene            complement(1705..>2846)
                     /locus_tag="PK977_09220"
     CDS             complement(1705..>2846)
                     /locus_tag="PK977_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRF18338.1"
                     /translation="LAFFRAYSGHLDAGSYVLNVRSGKKERISRIMKMFANKQNPIDF
                     IEAGDIGAAVGFKEIKTGDTLCDENHPITLENMFIPEPVIAVAVEPKTQADVDKMGMA
                     IAKLVEEDPTLRVNTDEETGQTILRGMGELHLEIIIDRMRREFKVEVNQGAPQVAYKE
                     AFTSTVEHRETLKKQTGGRGKFADIVFNLGPVDAEWQAENPDKHYQFVNDLFGGSIPR
                     EFVPAIQKGFEQAMTSGVLANYPVANMKIRVFDGSFHAVDSDSMSFELCAKQGFREAA
                     RKAKPVLLEPIMKVEVVTPDQYMGDVTGDLNRRRGMMEGMDSRAGAQVIKAKVPLSEM
                     FGYVTQLRSLSSGRASSTMEFSHYAPAPNNIAEEVIAKVKGKVSA"
     assembly_gap    2847..3020
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3021..3850)
                     /locus_tag="PK977_09225"
     CDS             complement(<3021..3850)
                     /locus_tag="PK977_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF18339.1"
                     /translation="MADLKFQRNFGIAAHIDAGKTTTTERILRYTGMIHRIGEVHDGA
                     ATTDWMEQEKERGITITSAAVSCKWNFPTVLGKNTADTKEYYFNIIDTPGHVDFTVEV
                     ERSMRVLDGLIALFSAVDGVEPQSETVWRQANRYGVPRIGFVNKMDRAGADFLNVVKQ
                     VKEMLGSKAVPLQLPIGAEDNFKGVVDLIKMKGIIWHMETEGMTFDEVDIPADMVEEA
                     NEWRANLVEAVAEYDDTLMEKFFEDPNSISEGEIHEAIRKATIDLSIVPMMCGSSFKN
                     "
     gene            complement(4404..5801)
                     /locus_tag="PK977_09230"
     CDS             complement(4404..5801)
                     /locus_tag="PK977_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF18340.1"
                     /translation="MKKIISALFALYLLLPASYGQNDEIRPQALGLSFFLNDFVTPAR
                     IRTTSLNTVIRDKSFARMRDMSPGLAVNYFRGVAKHVDIAASLGGSFVRYPMPNRNFS
                     SNNLLLEANASINLKMTTEKYWVQPYISVGVGGHKYRSYYGAFLPVGLGMKVNILDEA
                     HMFITSTYRVPVTTETANYHLQHAIGIAGSLKKKKVEPVILDRDKDGIPDMADACPDD
                     PGPAEFQGCPDRDGDGIPDKDDKCPDVKGLAKYQGCPIPDTDKDGINDEEDKCPTVPG
                     LARYQGCPIPDTDKDGINDEEDKCPTVPGVARYQGCPIPDTDGDGVNDEEDKCVTIPG
                     PKENFGCPIISEEVKKRVDFAAKNILFVTGSFKLQSKSFKGLNDVAKIMTENPGMSLD
                     IAGHTDNVGADDKNMILSQNRANAVKNYLVSKGVDASRISATGYGETMPIATNATAAG
                     RQQNRRSELKLSYYK"
     gene            complement(5958..>6722)
                     /locus_tag="PK977_09235"
     CDS             complement(5958..>6722)
                     /locus_tag="PK977_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRF18341.1"
                     /translation="IAISLDKYKQLPGQMPGNYIWVNAAANNLELVVNGKVELSSKVI
                     TGKPKTRTPMLTSAINSLITYPQWVPPPSIVSKEILPAVKKNPGYLQKKGFSLLDKDG
                     NEVDPYTVEWTKYSKGVPYRIVQGSGDANALGIMKFVFDNKYSVYLHDTNQRYLFANA
                     MRSLSHGCVRVQQWQDLAFWILRNDSGNPAGGSSRIDSVKTWLQNKQKRNIPLRNKVP
                     VFIRYITCEGKDGRVVFYDDVYGDDKRLREKYFAAK"
     assembly_gap    6723..6775
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6776..7660)
                     /locus_tag="PK977_09240"
     CDS             complement(<6776..7660)
                     /locus_tag="PK977_09240"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRF18342.1"
                     /translation="MSRNRYQSIYPSLTALLLVIFVFFACKSNKNTSDTIADTPAEMQ
                     QKAADLIKEYLVKTSADSGRSIDSSVLLRQSRLSRLLYEKQSYAPFWSKEEKWLPWGD
                     SMFLFIKESKLFGLFPEDYHFEQLQNIRERIFADSLSKGDRKDALLWSKADIMLTDAF
                     FQVVKDIKLGRLPNDSVTLRKDTVLTDEFYWQQLEKLKQNETIHEVAQKLEPSHLGYH
                     RLKNSIPHFLGSAENKQYTLVPYPEKDAVKFQRALQKRLYEGGYIAFDSAAADSAQLA
                     QAVKKFQQEKGITVDGRAG"
     gene            7723..8451
                     /locus_tag="PK977_09245"
     CDS             7723..8451
                     /locus_tag="PK977_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRF18343.1"
                     /translation="MNNNIRNRFRISRSLLVIFLITASLVACGQAGGYTRPAVTENYD
                     DYKKQVKADPGKKMIELRKLIPNIIYDLRYASVNNFMKRKMYPAGTNITFLRTPAAKA
                     LMQAQDELNQKGLGLKIFDAYRPYSVTVKFWELVKDERYVANPSKGSGHNRGTTVDIT
                     IINLQTKKELNMGTDFDNFSDTAHHGFTALPEDVLQNRILLKTTMEKNGFKPYNDEWW
                     HYSFSDGKQYEILDIDFKKLKKDL"
     gene            complement(8446..8745)
                     /locus_tag="PK977_09250"
     CDS             complement(8446..8745)
                     /locus_tag="PK977_09250"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HRF18344.1"
                     /translation="MPIIYHITTAADWNNAKMKGLYDHSSLKEEGFIHCSQESQIAGV
                     LERYFAGQTDLVKLVIDTDKLTSKYVFDWSPSTQDTFPHVYGPINIDAVIDVVPL"
     gene            complement(8745..>9513)
                     /locus_tag="PK977_09255"
     CDS             complement(8745..>9513)
                     /locus_tag="PK977_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRF18345.1"
                     /translation="GFPITILFENNNTRSEDYDKQRSIPRPGHADWVAHQKFGGNEDY
                     RGGGHFSARLTTGIVAAGAIAKKIMNGVSIKAEVTEIGGEKDLEKGLQKAIDAKDSVG
                     GIVECRVNGLPVGLGEPYFDSVESVLAHLVFAIPAVRGIEFGTGFAAAKMFGSEHNDA
                     IENPQGKTRTNHAGGVVGGISNGNELIFRLAVKPTSSTPKEQNSLNWDTGKTEDFSIK
                     GRHDLCVALRAPVIVEAVTAIVLADFMLLEQKIKRVF"
BASE COUNT         2585 a         2092 c         1747 g         2862 t
ORIGIN      
        1 ccgccattgc attccatcat catcagtgca ttttgtcctc cggttacctt acagttcttt
       61 actattgtat taatgcaatt acgcaaatac atggcagcgc cataacgaag ccactcatca
      121 ttttcattat gatggtaact catccagtca gaaatatctt ctttttcctg ggtgctgtta
      181 agatgctggc gatagttata actaaaatca cagttctcca gaacaagttt ctctacattt
      241 ttggccagca atgcaacttt ataacctctt gccaccaggt tttttatggt aatgttgcga
      301 ccattacgga tcaatattgc gacgccaaag aactcatcag gtcgaagctt ttgattactt
      361 ccctgtaata cagcctggtt aaaatcaatc gtaatattct ccccttctat caggacaaca
      421 ggtttatcat tgtcagaaag cccatccagt ttatactcct tttttagcac ttttccggat
      481 cgggatattt tcatgcccga aaccagtttt atattctgag catttaaaaa caatgaacaa
      541 agaagaagta ccaggatagc ttttattctt tttgtcatca atttgaattt acttgcaaca
      601 atttactgca ttatgcaaga acagtcaaag caaccttcaa gagtttcaaa atggcttaag
      661 ctgctattga aaattgcggt aatgactgta tgcctatggt atgtatccgg aaaaattgat
      721 tttgaaagat caaaaacggt tctgcaacat tcaaactggt taatactttt tattgctctc
      781 ttattatttg ttgtttccaa attcgtctct gctatccgcc tgaacattta ttttaagaac
      841 atagggatta tactatctga ggttgagaat atccggctct acctgctggg aatgttttac
      901 aacttgtttc ttcccggttc tgtaagtgga gatgcgtata aagttgtttt actgacaaaa
      961 cgatattcta tcccttataa aaagacgact gcagctgttt tactggatcg cttcagtggc
     1021 ttactggggc ttggaatatt attgacaacc tgcagttaca ttgtcctcaa tttcatttac
     1081 tgcctgattc taactgcaat aactgttgga gccattatag gtttctatta cattattaaa
     1141 cgatggttca aagattttat gtctggtttt tggccaacgc ttctcttagg gatattagta
     1201 caggcagtac aggtgatttg tatatatatg atcattcagt cccttggcgt tgaaaaaaac
     1261 aatgcagttt atattttcat tttcctggtt tcttctgctg tatccgtatt gccacttaca
     1321 attggcggcc ttggtattag ggaagtagtg ttcctggaag gagcggccta tttcagtttg
     1381 gataacgaaa catcagttat aatcagcctg ttattttatc ttattacatt ggtagtttca
     1441 gctaccggcc tgggatatat ttttaaagat tctcttaaaa aaaaagaaag gactcaataa
     1501 tgagtccttt accttgaaac cttgagcaaa tgattttcac aaccttaacc aggaagcctt
     1561 gaatcttaaa aacggggaaa tcgttaattg aactaacaat tttagtgcca ttcattcaat
     1621 cttgacttga cctggattaa atttcaaaaa taacccgttc tggctaatcc ggaacgggtt
     1681 attcgagatg ttattttttt tcaattaagc actaaccttt cctttcacct tagctatcac
     1741 ttcctcagcg atattgttag gtgcaggagc gtaatgagag aactccattg ttgaagaggc
     1801 acggccagaa ctcaatgaac gaagctgggt tacataacca aacatttcgc tcaatggtac
     1861 tttagccttg attacctggg caccggcacg gctatccatt ccttccatca taccacgacg
     1921 acggttaagg tctcctgtta catcacccat atactgatct ggagtaacta cttccacttt
     1981 catgataggc tccagtaata ctggcttggc ctttctggct gcttcacgga aaccttgttt
     2041 ggcacacaat tcaaatgaca tagaatcaga gtcaactgcg tggaagcttc cgtcaaatac
     2101 acggattttc atattcgcaa cagggtagtt agccaacact ccacttgtca tagcctgctc
     2161 aaaacctttt tgaatagcag gaacaaattc ccttggaatt gatccaccga acaagtcatt
     2221 tacaaactgg taatgtttat cgggattctc tgcctgccat tcagcatcca caggtccgag
     2281 attgaaaaca atatcggcaa acttaccacg accaccggtt tgtttcttca atgtctcacg
     2341 gtgctctaca gttgaagtaa acgcttcttt ataagctacc tggggtgcac cctggtttac
     2401 ttctacttta aattcacgtc gcatacggtc aataatgatt tccaggtgta actcacccat
     2461 accacgaagg atagtctgac ctgtttcctc atctgtattt acacgcagtg taggatcttc
     2521 ttctaccagt ttggcaatag ccatacccat tttgtcgacg tctgcctgtg ttttaggctc
     2581 aaccgcaaca gcaattaccg gctcaggtat gaacatattt tcaagggtga ttggatggtt
     2641 ctcatcacaa agtgtatcac cagttttgat ctctttaaat cctacagcag cgccaatatc
     2701 accagcttcg atgaaatcaa tagggttttg cttgttagcg aacatcttca taatacggct
     2761 gatcctttcc ttctttccgc ttcttacatt taatacgtaa gaaccggcat ccaggtgccc
     2821 ggaataggca cggaagaagg ccaggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn ttgttcttaa atgaagaacc acacatcatt ggtacgatgc
     3061 tgagatcaat ggttgctttg cggatcgctt catgaatttc tccttcactg atactgttgg
     3121 gatcctcaaa gaatttttcc atcaatgtat catcatattc agccacggct tctacaaggt
     3181 tagccctcca ctcatttgct tcttctacca tgtccgctgg aatatccact tcatcaaaag
     3241 tcattccctc tgtttccatg tgccagatga tccctttcat tttgataaga tcaactacac
     3301 ctttgaaatt atcttcagcg ccaataggca actgcagagg aacagcttta gagccaagca
     3361 tttcttttac ctgctttacc acattcagga agtcggcacc ggcacggtcc attttgttta
     3421 caaacccgat acggggaaca ccgtaacggt tcgcctggcg ccatactgtc tcagactgcg
     3481 gttcaacacc gtcaacggca gaaaacaggg cgatcaggcc atccagtacc cgcatggaac
     3541 gttctacctc tacagtaaag tcaacgtggc caggagtatc aattatattg aaatagtatt
     3601 cttttgtatc agctgtattc tttcctaaca cagtaggaaa gttccattta caacttaccg
     3661 cagccgaagt aatagtgata cctctttctt tctcctgctc catccagtct gtagtagcgg
     3721 cgccatcatg cacctcacca atgcggtgaa tcatacctgt gtagcggagt atacgctccg
     3781 ttgtagtggt tttaccagca tcgatatgag ctgcaatacc aaagtttctt tgaaatttca
     3841 gatctgccat gactatttga tttgtttttt gtgaggctca gcatctgcga tcgcctcctt
     3901 agtttttgat ttttattttc tgtttactta tttgatttat tttatcatcg cccggtttcc
     3961 tgccttcatt tgttctattg ctctcgaaat ttacccgtcg tctccccgaa caatgcgttg
     4021 cgttattttc acttaggaag atcagctaaa gaaagatgtg gttccatgac tggaaacatt
     4081 agctgatatg tagattagac tctcatagac cgccacttga agaaaaccac cttcaccccc
     4141 ttatcaaacc acctgtagct tttcttaatg gcggcgcaaa ggtagtggaa ttttctaaaa
     4201 aacaaataga caaatcgaga taatatcaac aggaaaccat ctaaataaac agattggcag
     4261 acttagtatc tcatttattt acaggcattt gctacttttt acaattcaaa gttcttcttt
     4321 gattaggata cacttcttat aataataaat gaaaatgtcc cgggctccgg gacattttca
     4381 tttattaact actaatattc aaattatttg tagtaactga gttttaactc agaacggcgg
     4441 ttttgttgac ggccggctgc tgtagcatta gtggctattg gcattgtctc accataaccc
     4501 gtagcagaaa tgcggctggc atccacacct ttacttacca ggtagttctt aactgcatta
     4561 gccctgttct ggcttaatat catattctta tcatcagcac ctacattatc agtgtggcct
     4621 gcaatgtcca ggctcatacc gggattctca gtcatgatct tagctacatc attcaggcct
     4681 ttaaacgatt ttgactggag tttaaaactt ccggtaacaa agagaatatt tttagcagca
     4741 aaatctactc ttttcttcac ttcttcagaa ataatcgggc aaccaaaatt ctccttagga
     4801 ccaggaatgg ttacacactt atcttcttca tcatttacac catcaccatc tgtatccggt
     4861 atcgggcatc cctggtaacg ggcaacaccc ggaacggtag ggcatttatc ttcttcatca
     4921 ttgataccgt ctttatctgt atccggtatc gggcatccct ggtaacgggc caaacctgga
     4981 acagtaggac atttatcttc ttcatcattg ataccgtctt tatctgtatc cggtatcgga
     5041 catccctggt atttggcgag gccttttaca tctggacatt tatcatcttt atcaggaata
     5101 ccgtctccat ccctgtcagg acatccctgg aactctgcag gtcccggatc atcgggacaa
     5161 gcgtctgcca tatcaggtat tccatcttta tctctgtcaa gtattacagg ttctactttt
     5221 ttcttcttca aacttccggc aataccgatt gcatgctgta aatgatagtt tgctgtttca
     5281 gtcgtaacag gcaccctgta tgtagaagta atgaacatgt gagcctcatc aagaatattc
     5341 actttcattc ccaaacctac cggcagaaat gcaccataat aggaacgata tttatgacca
     5401 ccaacaccga ctgatatata aggctgcacc caatattttt cagtcgtcat tttcaggttg
     5461 attgaagcat tggcttccag cagaagatta ttactagaga aatttctgtt gggcattgga
     5521 taacgaacga acgaaccacc caatgaagct gctatatcaa catgcttggc aacaccacgg
     5581 aagtaattca cagccaaacc cggagacata tctctcatcc tggcaaagct tttatctctg
     5641 ataacggtgt tgagggatgt tgtacggata cgggcaggag taacgaaatc atttagaaaa
     5701 aaactaagac ccagagcctg gggcctgatt tcatcgtttt gcccgtaact ggccggtaaa
     5761 agcagataca gcgcaaacaa tgctgatatt atcttcttca taaagagata gtttggtttt
     5821 aaatctggac aaaaataacg gctaagttca atatccgaaa ataaattatt tacttaatat
     5881 tcaccgtttt gggaggcttt ctttttaggt aaaaaggggc ttttaggggc aaacccgaac
     5941 aacatgtgaa taaagcatca tttggcagca aaatatttct ctctcaacct cttgtcgtcc
     6001 ccgtacacat catcataaaa taccacccgc ccatccttcc cttcacaggt tatataccgg
     6061 ataaaaacag ggaccttgtt gcgaagtggt atatttcttt tttgcttgtt ctgcaaccag
     6121 gtttttacgg aatcaattct ggaagagccg ccggcaggat tgccgctgtc atttcgtaaa
     6181 atccagaaag caaggtcctg ccattgctgc accctgacac aaccgtggct cagtgaccgc
     6241 atggcattgg caaaaagata ccgctggttg gtatcgtgca agtaaacgga atacttattg
     6301 tcaaatacaa atttcatgat accgagggca ttggcatcgc cgctgccctg tacaatccgg
     6361 tagggcaccc ctttgctgta tttcgtccat tcaactgtat aaggatctac ctcatttcca
     6421 tctttatcaa gcaggctaaa gccttttttt tgcaaatagc cgggattctt tttaaccgcc
     6481 ggcagaattt cttttgaaac aatactggga ggaggtaccc attgaggata agttatcagg
     6541 gaattgatgg cactggtcag catcggtgtt cttgtctttg gcttcccggt aatcacttta
     6601 gaagaaagct ccactttacc gttcactacc agttccaggt tattggcagc agcatttacc
     6661 caaatatagt tcccaggcat ctgcccgggc agttgcttgt atttgtccag gctaatggca
     6721 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgg
     6781 cacggccatc aacagttatt cctttttcct gttgaaattt tttaacggcc tgggccaatt
     6841 gggccgaatc cgccgctgca ctgtcaaaag caatataacc tccttcatat aatcgctttt
     6901 gcaaagccct ttgaaacttt acagcatctt tttccgggta aggcactaga gtatactgtt
     6961 tgttttcagc cgaaccaagg aaatgaggaa tcgaattttt caaccggtga taacctaaat
     7021 gggaaggctc cagcttttgg gcaacctcat ggattgtttc attttgtttc aacttttcca
     7081 gttgctgcca ataaaattca tctgtcagaa cggtatcctt acgaagggtc acactatcat
     7141 tgggcaaacg gcccagctta atatctttta ccacctggaa aaatgcatcg gtcagcataa
     7201 tatcggcttt tgaccacagc agggcatctt ttctgtctcc ttttgaaagg ctatctgcaa
     7261 aaatcctttc cctgatattt tgtaattgtt caaaatgata atcctctgga aacagaccaa
     7321 acaattttga ttctttaatg aacaggaaca tagaatctcc ccatggcaac catttttctt
     7381 ctttgctcca aaaaggggca taagactgct tttcatacag caaacgggat aaccgtgatt
     7441 gacgaagcaa aacagatgag tcgatagatc tgcctgaatc agcagatgtt tttaccaggt
     7501 actcttttat caggtcagca gctttttgtt gcatttctgc aggtgtatca gcaattgtat
     7561 cactggtatt tttattgctt ttacaggcaa aaaatacaaa tatgaccagc aacaaagcgg
     7621 tgagagacgg atagattgat tggtatcgat tcctgctcat agtggctgta acgaacctaa
     7681 tccggcattt tatattaact taccagggta acagtaccgg atatgaataa caatataaga
     7741 aatcgtttca ggattagcag aagcctgcta gttatttttt tgattacggc atcattagtt
     7801 gcctgcggac aagcaggtgg ttatacccga ccggctgtaa cggaaaatta cgatgattat
     7861 aaaaaacaag taaaggctga cccgggcaaa aaaatgattg agctgcgcaa gctgatccca
     7921 aatatcatct acgatttacg atatgcctct gtcaataatt ttatgaaacg gaagatgtac
     7981 ccggcaggca ccaatattac ttttctgaga acacctgctg ccaaagccct gatgcaggcg
     8041 caagatgaac tgaatcaaaa agggcttggt ttaaaaattt ttgatgcata ccgcccttat
     8101 tctgttaccg taaaattctg ggaactggta aaagatgaac gttatgttgc caatccttcc
     8161 aaaggaagcg gacacaacag ggggaccact gttgatataa cgatcatcaa ccttcagaca
     8221 aaaaaagaat taaacatggg aaccgatttt gataattttt ctgatacagc tcaccatgga
     8281 tttactgcat tacccgaaga tgttttacaa aaccgcatat tgcttaaaac cactatggaa
     8341 aaaaatggtt ttaaaccgta taatgacgaa tggtggcatt attcattttc cgacgggaaa
     8401 caatatgaaa tccttgatat tgattttaaa aagctgaaga aggatttata acggaactac
     8461 atcgataaca gcatcaatat taatcggccc atatacatgt ggaaatgtat cctgtgtgga
     8521 tggagaccag tcaaatacat acttgctggt cagcttatct gtatcaatta ccagtttcac
     8581 caaatcagtt tggcccgcaa aatatctttc cagcacaccg gctatctgac tctcctgtga
     8641 gcaatgaata aagccttctt cttttaaaga agaatggtca tacagtccct tcatttttgc
     8701 gttgttccag tccgccgcag tagtaatatg ataaataata ggcattagaa tactcttttt
     8761 attttttgtt ctaataacat aaaatcagcc agcacaatgg cagtaacggc ctctacaatc
     8821 acaggggccc tgagtgccac acacaagtca tgacgtcctt tgatagaaaa gtcctctgtt
     8881 ttacccgtat cccagttcag gctgttttgt tctttgggtg tagaggatgt tggttttacc
     8941 gccaggcgga aaatcaactc atttccatta ctgataccac ctactactcc accggcatga
     9001 ttggttcttg tttttccttg cgggttttca atagcatcat tatgttcgct gccaaacatt
     9061 tttgcggcgg cgaaaccggt gccaaattca atacctctta ctgcaggaat agcaaatact
     9121 agatgtgcca gtactgattc aacggaatca aaatacggct cgccaagacc aacaggtaaa
     9181 ccattaaccc tgcattcaac aatgcctcct acagaatctt tcgcatcaat ggctttttgt
     9241 aatccctttt ccaaatcttt ttcgcctcct atttcggtca cttcagcttt tatacttacg
     9301 ccgttcatta tttttttagc tatcgcccct gcagctacaa tgccagttgt gagtctcgca
     9361 ctgaaatggc ctccgccccg atagtcttca ttacctccaa acttttgatg tgccacccag
     9421 tcggcgtggc cggggcgggg aatacttctt tgtttatcat aatcttcgct gcgggtatta
     9481 ttattttcaa ataaaatggt gatggggaaa ccg
//
LOCUS       DAORLS010000105         9493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3886_length_9493_cov_1.839267, whole genome shotgun sequence.
ACCESSION   DAORLS010000105 DAORLS010000000
VERSION     DAORLS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3886_length_9493_cov_1.839267"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>983
                     /locus_tag="PK977_09260"
     CDS             <1..>983
                     /locus_tag="PK977_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF18346.1"
                     /translation="DYPWMDEGINSYFEDKYIKANYGEADKSQQIAFESIAIEKIDQP
                     VATVSGQFSEINYNLVAYYKTAEWFRYLESEIGKEVLANAIKEYYRRWQFRHPQPEDF
                     KKVIEEVSGRKLDNEFSLLDKKGILPNQQRKGTKIFVLLKPKGLNSYLKKPSKELITF
                     GPIIGANSYDKFMAGAFITNIKLPPSRLQYMLSALYATGSKKMTGTGFITQSFYPDRL
                     FRKIDVGISGSTFTVDQFTSSDGEKLFLGMKKIVPGIRFTFKEKSPRSSFHRYIQFKT
                     FLFNEDGLRFYRDTLITGNDTTITNRFGKTSKSRTLNQLKLVIENNRVLYP"
     assembly_gap    984..1048
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1049..>1351
                     /locus_tag="PK977_09265"
     CDS             <1049..>1351
                     /locus_tag="PK977_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18347.1"
                     /translation="YFFNYANVGGLNVRLFAGKFFYTSAKTFATQFATDRYHLNLTGA
                     NGYEDYTYSDYFIGRNKFEGLASQQIMNRDGGFKVRTDLLADKVGKTDDWLIAANF"
     assembly_gap    1352..1397
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1398..1685
                     /locus_tag="PK977_09270"
     CDS             <1398..1685
                     /locus_tag="PK977_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18348.1"
                     /translation="VLPVKIPLKLFFDIGTYAESWKEEAETDRFLFDAGIQIPLVKET
                     INIYLPLIYSSVFKDYIQSTIPKKGRLWKTISFTIDISNFSFRKFDRNLNF"
     gene            1685..1894
                     /locus_tag="PK977_09275"
     CDS             1685..1894
                     /locus_tag="PK977_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18349.1"
                     /translation="MGEQYNIRYLTHREIDKAKWDYRVSSSSNGLIYAYSYYLDTMSK
                     NWDALVLNDYEAIMPLVWNKKNRGY"
     assembly_gap    1912..2052
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2053..2615
                     /locus_tag="PK977_09280"
     CDS             <2053..2615
                     /locus_tag="PK977_09280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF18350.1"
                     /translation="SLYQRSNFVLSLESTYEELFRNYRENIKRNIKKAIQVGCSAETG
                     FEEEKVIQLAMQQMKNQSKETTDNINRFRKLYKILYEKGMATTYGILSVQKELLASAI
                     FFYSHNRAYYILVGNHPNGKTIGASHFLIDSFIKDHAGKKIVLDFEGSDIRNLAFFYS
                     SFGAVEEKFAAIRLNKLPFYLKWLKK"
     gene            complement(2617..3285)
                     /locus_tag="PK977_09285"
     CDS             complement(2617..3285)
                     /locus_tag="PK977_09285"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRF18351.1"
                     /translation="MKKYAIVVAGGSGTRMGTSLPKQFMLLRDKPVLYYTLKVFLEAY
                     DDLEIVLVLPVDYTDMGQEIIDAFFDKERIRITAGGDTRFQSVKNGLSLIDEESIIFV
                     HDGVRCLVTKELIQRCYLQAVETGTAIPAVPSKDSIRLITEEGNEAFDRNKVMLVQTP
                     QTFHSKILLPAFQIDYKDKFTDEATVVEAYGMKVSLVEGEVNNIKITRPVDLLIAESI
                     LSGK"
     gene            complement(3290..4261)
                     /locus_tag="PK977_09290"
     CDS             complement(3290..4261)
                     /locus_tag="PK977_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRF18352.1"
                     /translation="MSHYTLVIHGGAGTISKSNINAEQESAYCDALGKALEAGFSCLK
                     NDGTALDAVRAAVVSLEDCILFNAGKGSVFGKDGRQEMDASIMDGKTLMAGAVAAVRN
                     IRNPVELAYTIMTKSQHVMLNGSGAYEFAVQQGLAIEPDDYFFSQFRYDQWKEIQGTN
                     NAALDHNVSVRDKKFGTVGAVALDKHGNIAAATSTGGMTNKQFGRIGDSPIIGAGTYA
                     NNKTCAISCTGHGEPFIRAVTAYDVSCLMEYKEFSLSEAMHVVVHDKLLKIDGEGGMI
                     GVDAKGNATRVGRRRNTDGNAAMVFNSEGMYRGMMNAEGKKEILIYK"
     gene            4324..4998
                     /locus_tag="PK977_09295"
     CDS             4324..4998
                     /locus_tag="PK977_09295"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HRF18353.1"
                     /translation="MFRKKLTLADWILIVANMVPVFGVWFLGWDAVEAFIVYAMETMV
                     VGILTVGKLLVATFARGGDEWPANGTVSRQSGFFFIIFFILHFGIFALVQTTIFSQTA
                     GITPPGSHTLHFFFKWYTYINRDIAYMLAAFIISYLAKSFIPYILNDEYRTTSFMIIM
                     FQPYGRIFIQQFTVILGSMFLTLGAGKIFVLVFSAIKIAFEIFIDYDKMLNKTMENVR
                     NRSTKQ"
     gene            complement(4984..5973)
                     /locus_tag="PK977_09300"
     CDS             complement(4984..5973)
                     /locus_tag="PK977_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRF18354.1"
                     /translation="MIDFQQLFSSFSSLKVGVIGDVMLDTYMWGKVDRVSPEAPVPVV
                     SLQRKEYRVGGAGNVALNCRSLAAQVTMLSVAGNDEEAVILKKLLDESEIDTSYMLFS
                     DRRITTNKTRIISRNQQMMRLDAETTDDLDKELQEQFIEKIILYISNEEPDIIIFEDY
                     NKGVLTEKLITDIIQVCKNKTVITSVDPKKKNFFAYKGVDIFKPNLKEVKEALNFQVD
                     ELSVPVLENIHSELLRFLDHRISFITLSEKGVFYQQEKGGHIIPSHMRNIADVSGAGD
                     TVIAVASLVYAATKDVKLMADIANIAGGLVCEEVGTVTINKEKLLKECQSLLG"
     gene            complement(6052..6657)
                     /locus_tag="PK977_09305"
     CDS             complement(6052..6657)
                     /locus_tag="PK977_09305"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF18355.1"
                     /translation="MKNGILIWNVVLTLVAGYLLYSHFDSKKTKPSSYSGKGNNTADN
                     TAFKMAYFEMDSVAANFELVKEVKAELNKKEDEIATEMDRLARKIQDKYAYYQNLAQA
                     GNLSETQSEAASLEIKKMDDEMKARKQQLDQDYFDLKTRRETDIKTKIEGFCKEYNSK
                     HNYTYIISYEQGLFFYRDTAYNITAELVKGLNEKYKPEKQK"
     gene            complement(6807..>7870)
                     /locus_tag="PK977_09310"
     CDS             complement(6807..>7870)
                     /locus_tag="PK977_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRF18356.1"
                     /translation="RDKTKIPSIVVEGKEKKSYNLPTGSHIIMEEGDDVKAGQVIVKI
                     PRVLGKLRDITGGLPRVTELFEARNPSNPAVVSEIDGVVMMGAIKRGNREIIIEAKDG
                     VQKKYLVPLTRQILAQDGDFVKAGASLSDGQIAPFDILSIKGPFAVQEYVVNEIQEVY
                     RLQGVKINDKHIEVIVRQMMRKVNIVDPGDTRFLEDDLVDKFEFVEENDYIFDKKVVT
                     DVGDSTKLRAGQIVTLREVREENSILRRNDKKPVEYRDAKPATSSPTLLGITKASLGV
                     QSWISAASFQETTKVLSSAAINGKTDEMLGLKENVITGHPIPAGTGLREFENMIVGSK
                     EEYELLQTTKEAMAFDEEE"
     assembly_gap    7871..7941
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7942..>8332)
                     /locus_tag="PK977_09315"
     CDS             complement(<7942..>8332)
                     /locus_tag="PK977_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRF18357.1"
                     /translation="QSIGEPGTQLTLRTFHVGGVAGSASVESTLTAKFDGTIQFDGLR
                     TVAIENNEGEKTQVVIGRTGEVRIIDTKNDRLLITNNIPYGATLNVKDGQKVNKGDVI
                     CTWDPFNNVIMAEINGTVKFENVIDGVT"
     assembly_gap    8333..8348
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8349..>9493)
                     /locus_tag="PK977_09320"
     CDS             complement(<8349..>9493)
                     /locus_tag="PK977_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRF18358.1"
                     /translation="FYSSEEVIIAYNERVVDLHAWIKVKANIRNADGLLEHKLIETTV
                     GRVIFNQHVPAEVGFVNALLTKKNLREIIGDIIKITNVPKTAKFLDDIKQLGFRTAFQ
                     GGLSFNINDLIIPDVKEELLENAKGEVDEVWDSYNMGLITNNERYNQIVDIWSRVDTR
                     ITETLIKELSSDKQGFNSVYMMLDSGARGSKQQIKQLAGIRGLMAKPRKSGSTGSEII
                     ENPILSNFKGGLNVLDYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVVITEED
                     CGTLRGIATTALKDNEDIIEPLSDRIAGRTSLHDVYDPISDMLIVSAGEEITADIARK
                     IEDAGIETVEIRSVLTCESKRGVCVKCYGKNLASGNLAQKGDAVGII"
BASE COUNT         2644 a         2078 c         1626 g         2806 t
ORIGIN      
        1 gagactaccc ctggatggat gagggcataa acagttattt cgaagataaa tatattaaag
       61 ctaactatgg cgaagcagac aaatctcagc aaattgcgtt tgaatcaata gcaatagaaa
      121 aaatcgatca acccgttgct actgtttccg ggcagtttag tgagattaat tataaccttg
      181 ttgcctatta taaaaccgcc gagtggtttc gttacctgga atccgaaata ggaaaagaag
      241 tactggcaaa tgcaataaaa gaatactatc gccgctggca gttcaggcac ccccaaccgg
      301 aagactttaa aaaagtaatt gaagaagtaa gcgggagaaa acttgataac gagttttctc
      361 tccttgataa aaaaggaatt ttacccaacc agcaaagaaa aggcaccaaa atatttgttt
      421 tactcaaacc aaaagggctt aattcatatc tgaagaagcc ttctaaagaa ctaataactt
      481 ttggtcctat cattggtgca aatagttatg ataaattcat ggccggggcc tttataacca
      541 atataaagct gccaccatcc aggttacaat acatgctgtc tgcgctatat gctaccggtt
      601 ccaaaaaaat gaccggcaca ggctttatca cacaaagctt ttatcctgat cggttattcc
      661 ggaaaataga tgtaggcatt agtggctcca cttttactgt tgatcagttt accagttcag
      721 atggagaaaa attgtttctg ggaatgaaaa aaattgtacc tggcattcgc tttacattta
      781 aagaaaaaag tccacgaagc agttttcacc gatatattca attcaagact tttttattta
      841 atgaagacgg actacggttc tacagggaca cccttattac aggaaatgat accactatta
      901 caaatagatt tgggaaaacc agcaaaagca gaacattaaa ccagcttaaa ctggtgattg
      961 agaataacag ggtactatat cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt actttttcaa ctatgcaaat gtaggagggc
     1081 taaatgtgag gctgtttgca ggaaaattct tttatacttc tgcaaaaact tttgccacac
     1141 aatttgctac cgatcgttac cacctcaatc ttacaggggc aaacggttat gaggattata
     1201 cttatagtga ttattttatc ggtcgaaaca aatttgaagg gcttgctagc caacagatca
     1261 tgaacaggga cggcggcttc aaagtaagaa ctgatctgct ggcagataaa gttggtaaaa
     1321 cagatgactg gctgatagct gccaatttca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnngtc ttaccggtta aaattccgct taagttattc tttgatatag
     1441 gcacttatgc tgaatcatgg aaggaagaag cggaaacaga ccgtttcctg tttgatgcgg
     1501 gcatacaaat tcccttagtt aaagaaacaa tcaatatcta tctgccattg atctatagca
     1561 gtgtatttaa agactatata caatctacta tcccaaaaaa gggaagactg tggaaaacca
     1621 tttcttttac aatagatata tccaatttca gcttcaggaa atttgaccgt aaccttaact
     1681 tttgatgggt gaacaataca acatacgata tcttacccac agagaaatag ataaagcaaa
     1741 atgggactac cgggtatcga gttcatccaa tggactgatt tatgcctatt cttactatct
     1801 cgataccatg tcaaaaaact gggatgcatt ggtcttaaac gactatgagg caataatgcc
     1861 cctggtatgg aataaaaaaa acaggggata ttaactgtac cagccttttt tnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nntctctctt taccagagat caaactttgt actcagcctt gaaagcactt
     2101 acgaagagct attcaggaac taccgggaga atattaagcg aaatataaag aaagctatac
     2161 aagtaggctg ttccgctgaa acaggatttg aggaagaaaa agtgattcaa ctggcaatgc
     2221 agcaaatgaa aaatcaatcg aaagaaacaa ctgataatat aaaccgattc agaaagcttt
     2281 acaaaatact ttatgaaaaa ggcatggcaa ctacctacgg aattttatca gtacaaaaag
     2341 aattactcgc atccgctatt tttttctatt cgcataaccg tgcttactat atattagttg
     2401 gtaaccatcc gaatggcaag accatcggcg cctcccattt ccttattgat tcttttatta
     2461 aggatcatgc cggcaaaaaa atagtactgg actttgaagg atccgacatt cgaaaccttg
     2521 cattttttta tagcagtttc ggagcggtgg aagaaaaatt tgctgcaatc aggctaaaca
     2581 agcttccttt ttatctgaag tggcttaaaa aataagttat tttccgctca atatactttc
     2641 tgctatcaaa aggtcaacag gtcttgtaat tttaatatta tttacttcac cttcaacaag
     2701 cgacactttc atgccatagg cttctacaac agtagcctca tcagtaaact tgtctttata
     2761 atcaatctga aaagctggaa gcaatatttt gctatgaaaa gtttgtggcg tttgcaccag
     2821 catcacttta tttctgtcaa aagcctcatt accctcttct gttatcaaac gtatgctgtc
     2881 tttagaagga actgccggaa tagcagtacc ggtttccacc gcctgcaggt aacatcgctg
     2941 tatcagttct ttcgtaacca ggcacctcac tccatcatgt acaaaaatga ttgattcttc
     3001 gtcaataagt gagagaccgt tctttaccga ttgaaaccgg gtatccccac cagccgtgat
     3061 cctgatcctc tctttatcaa agaaagcatc aataatttcc tgccccatgt cagtgtaatc
     3121 aacaggcaat accagtacta tctcaagatc atcgtaagcc tccaaaaaca cttttaatgt
     3181 ataataaagt acaggcttat ccctgagcag cataaactgt tttggcagac tggtccccat
     3241 tcttgtacct gaacctccgg caacaactat agcgtacttt ttcatttctt tatttataaa
     3301 tcaaaatttc cttttttcct tcagcattca tcatacccct gtacattccc tcactgttga
     3361 ataccatagc agcgtttccg tctgtgtttc ttctgcggcc cacgcgtgtt gcgtttcctt
     3421 ttgcatccac tccaatcatt ccaccctcac catctatttt cagcaatttg tcatgtacta
     3481 ctacatgcat tgcctcactg agagaaaact ctttgtattc cattaggcaa ctcacatcat
     3541 atgctgttac tgcccgtatg aatggctctc catgtcctgt acaactgata gcacaggttt
     3601 tgttattggc ataggttcct gcaccaatta taggactgtc accgatcctg ccaaactgtt
     3661 tattggtcat accccctgta ctggtagcgg ctgctatatt accatgttta tccaacgcca
     3721 cagctccgac agttccgaat tttttgtctc taaccgaaac gttatgatca agtgcagcgt
     3781 tgtttgttcc ctgtatttct ttccactggt catatctgaa ctgtgaaaaa aaataatcat
     3841 ctggctctat ggccaatccc tgctgtacag caaactcata agcgccgctg ccgttaagca
     3901 ttacatgctg gctttttgtc atgatcgtat aagccagttc tacaggatta cggatatttc
     3961 ttacggctgc aacagctcct gccatcagtg tttttccatc catgatcgaa gcatccattt
     4021 cctggcggcc atcttttcca aaaacagatc ctttaccggc attaaacaaa atacaatctt
     4081 ccagtgagac aacagcagcc ctgacagcat caagagctgt tccatcattc ttcaaacagc
     4141 tgaacccggc ctccagtgct tttcccaatg catcacagta tgccgattcc tgttcggcat
     4201 tgatgtttga tttcgaaatg gtaccggctc ctccatgaat gacgagggtg tagtgagaca
     4261 tattgtaatt acgttaatta acgaacgaaa ttaggtattt tacagccgaa actgtttaac
     4321 ctgatgttca gaaaaaaact aacccttgcc gattggatat tgatagttgc aaacatggtt
     4381 cctgtatttg gcgtatggtt cctggggtgg gatgcagtag aagcatttat agtttacgcc
     4441 atggaaacga tggtagtagg aatacttact gtaggaaaat tactggtagc tacctttgcc
     4501 aggggcgggg atgagtggcc tgcaaacggt actgttagca gacaaagcgg attttttttt
     4561 attatcttct ttatactcca ctttggaata tttgcactgg tacaaaccac tattttttca
     4621 caaacagccg gcataacacc gcctggcagt catacattac atttcttctt caaatggtac
     4681 acttatataa accgggatat agcttacatg cttgccgcat ttattatcag ctaccttgcg
     4741 aaaagtttta tcccgtacat cctaaatgat gagtatagga ccacctcttt tatgattata
     4801 atgtttcagc cctatggcag gattttcatc cagcaattca ccgtaattct cggcagtatg
     4861 tttcttacac tgggagcagg aaaaatattc gttcttgttt tttctgcaat taaaattgcc
     4921 ttcgagatat tcatcgatta tgataagatg ctaaacaaaa caatggaaaa cgtaagaaac
     4981 aggtcaacca agcaatgatt gacattcttt aagcaatttt tctttgttga tcgtgacggt
     5041 gcccacttct tcacaaacca gcccaccggc tatattcgca atatcggcca tcagctttac
     5101 atctttcgta gcggcgtaaa caagagatgc tacagcaatg acagtatcac ctgcccctga
     5161 aacatctgcg atattccgca tatgcgaagg aataatatgc cccccctttt cctgctggta
     5221 aaaaactcct ttttctgaga gtgtgataaa agatattcta tgatctagaa atctcaaaag
     5281 ctctgaatga atattctcaa gcaccggaac agatagctca tctacctgaa aattaagtgc
     5341 ttcttttact tctttaaggt taggcttgaa aatatcaact cccttatagg caaaaaagtt
     5401 tttctttttg ggatctaccg aggtaataac agtcttgttc ttacatacct ggattatgtc
     5461 tgtgatcagt ttttctgtta gtacgccttt gttgtaatct tcaaaaatta tgatatcagg
     5521 ctcttcattg cttatgtata aaataatttt ctcaataaac tgctcctgca gctctttatc
     5581 caggtcatca gtagtttctg catccagccg catcatctgc tggttgcggc ttattattct
     5641 tgtcttattc gttgtaattc gcctgtcaga aaaaagcatg taggacgtat caatttcact
     5701 ttcatctaac agtttcttca gtattacagc ctcttcatca ttgcctgcta cgcttaacat
     5761 ggttacctgt gcagccaaag agcggcaatt tagtgcgaca ttaccggcac cccctaccct
     5821 gtattctttt ctttgtaatg aaacaacagg tactggtgct tcaggggaaa ctctatcaac
     5881 cttgccccac atataagtat ccagcatcac atcacctata acgcctacct taagtgatga
     5941 aaaggaagaa aaaagttgct gaaaatctat cataccccaa agaaagaatt atttcctttg
     6001 cttaccaaac gaaaacgtcc cattatggga cgtcctgttt ctgaattact attatttctg
     6061 cttctctggt ttgtattttt catttagccc tttaaccagt tccgctgtaa tgttataggc
     6121 tgtatccctg taaaaaaata aaccttgttc gtatgaaatg atgtaagtat aattatgctt
     6181 tgaattgtac tctttgcaaa agccttcaat tttcgtctta atatccgttt cccgtctagt
     6241 tttgaggtca aaataatcct gatcaagctg ctgtttgcgg gccttcattt catcatccat
     6301 tttcttgatc tccaggcttg cagcttcaga ctgtgtttca gaaagattgc ctgcctgggc
     6361 caggttttgg taatacgcat atttatcctg gattttccgt gcaagcctgt ccatttctgt
     6421 cgctatctca tcttcctttt tgtttagttc agcttttact tctttaacca gctcaaaatt
     6481 ggccgcaact gaatccattt caaaataagc cattttaaat gcagtattat cggcagtatt
     6541 attcccttta cccgaataag aggaaggctt tgttttcttt gaatcaaaat gggaatacaa
     6601 cagataacca gccacaaggg tcagcaccac gttccagatt aaaatgccat ttttcattca
     6661 gttatctttt tacaaaacta agtatgtaca ggccggcaaa tataagcaat cggttcactt
     6721 taggaaaccc ttaacagcca ccaaattgag taaaaaagga gtaagaaaat cttactcctt
     6781 cataatatca aaaagaccta atttaattat tcttcttcgt caaatgccat tgcttccttg
     6841 gtagtctgca gcaattcata ttcttccttg cttcctacga tcatgttctc aaactccctg
     6901 agacccgtac ctgcaggtat cggatggcca gttatcacat tctccttcag gccgagcatt
     6961 tcatcagttt taccgttaat ggccgcagat gaaagcacct tagttgtttc ctggaacgaa
     7021 gccgcagata tccagctttg cacacccaat gaagccttgg taatacccaa caatgtaggt
     7081 gaggaagtag caggcttggc atcacgatac tcaacaggtt tcttatcgtt tctgcgcagt
     7141 atagagtttt cctctcttac ttcccgcaac gttacgattt ggcctgcacg aagttttgta
     7201 ctgtcaccca catccgttac taccttttta tcaaagatgt aatcattctc ttccacaaat
     7261 tcgaacttat caaccaggtc atcttcaagg aaccttgtat ctcccggatc aacaatattt
     7321 actttacgca tcatctggcg aacgataact tcaatgtgtt tgtcattgat ctttacaccc
     7381 tgcaaacgat aaacttcctg gatttcattc accacatact cttgtacagc gaaaggtcct
     7441 ttgattgaaa ggatatcgaa tggtgcgatc tgaccatcac ttaacgaagc gccggctttt
     7501 acaaaatccc cgtcctgtgc aagaatctgt ctggtaagcg gaacgaggta cttcttctgt
     7561 acaccatctt tagcctcgat aatgatctca cggttaccac gtttaatggc tcccatcatc
     7621 acaacaccat caatctcaga aaccactgca ggattgcttg gatttcttgc ttcgaacaac
     7681 tcagttacac gaggaagacc tccggtaata tctctcagct tgccgagcac ccttggaatc
     7741 ttaacaatca cctgtcctgc ttttacatca tcaccctctt ccattataat atggcttccg
     7801 gtaggcaggt tatatgattt cttttccttg ccttctacta caattgaagg aatcttggtt
     7861 ttatctcttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn naggtaacgc catcaatcac attttcgaac ttcacagtac
     7981 cattgatttc tgccatgatt acgttgttga acggatccca tgtacagatc acatcaccct
     8041 tatttacttt ctggccatct tttacattca gcgtagcacc atacggtata ttattggtga
     8101 tcagcaggcg gtcattttta gtatcgatga tacgaacttc accggtacgt ccgattacta
     8161 cctgtgtctt ttctccttca ttattctcaa tggcaacagt acgcagacca tcgaactgga
     8221 tagtgccgtc aaactttgca gtcagtgttg attctacaga tgcagaacct gccacaccac
     8281 ctacgtggaa tgtacggagt gtcagctgtg taccaggctc accgatagac tgnnnnnnnn
     8341 nnnnnnnnca atgataccta ctgcatcacc tttttgtgca agattaccag atgcaagatt
     8401 tttaccataa cacttcacac atacacctct cttgctttcg caggtaagta ctgaacgtat
     8461 ttcaactgtt tcaattcctg catcttcgat cttccttgcg atgtcagcgg taatttcttc
     8521 gccagctgaa acaatcagca tatcgctgat agggtcatat acatcatgta aagatgtacg
     8581 tcctgcaatc ctgtcgctca gtggctcaat gatatcctca ttatctttta atgcagttgt
     8641 ggcaatacca cgcaatgttc cgcaatcctc ttcagtgatt acaacatcct gggcaacgtc
     8701 taccagtcga cgggtaaggt aaccagcatc ggctgtttta agggccgtat cggccagacc
     8761 cttacgggca ccgtgggtag aaatgaagta atccaatacg ttcaaacctc ccttaaagtt
     8821 tgaaagaatg gggttctcaa tgatctcaga acctgtagag ccggactttc tcggcttggc
     8881 catcaatccc ctgatacctg ccaactgctt gatctgctgc ttagatcccc ttgcacctga
     8941 atcaagcatc atatatacag agttgaagcc ttgcttatca ctgctcaatt ccttgatcag
     9001 cgtttcagta attcttgtat ccacacggct ccagatatct actatttggt tataacgttc
     9061 gttgtttgta atcagaccca tgttatagct atcccatacc tcatcaactt caccttttgc
     9121 attctccagc aattcttctt tcacatccgg gataataagg tcattgatat taaacgaaag
     9181 accaccctgg aatgctgtac ggaaacctaa ttgtttaata tcatccagga actttgcagt
     9241 cttaggaaca ttggtaatct tgatgatgtc gccaatgatc tcccgcaagt tcttcttggt
     9301 cagaagggca tttacaaatc ctacttcagc aggcacatgc tggttgaaaa taacacggcc
     9361 tactgttgtt tcgataagct tatgctccaa caatccgtca gcattgcgga tattggcttt
     9421 cacctttatc catgcatgca ggtcaactac cctttcatta taagcgataa taacctcttc
     9481 acttgaataa aat
//
LOCUS       DAORLS010000106         9456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3903_length_9456_cov_1.891714, whole genome shotgun sequence.
ACCESSION   DAORLS010000106 DAORLS010000000
VERSION     DAORLS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_9456_cov_1.891714"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK977_09325"
     CDS             <1..211
                     /locus_tag="PK977_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF18359.1"
                     /translation="IVISKNLSTYEDLLNSYGFIRTHHSYLVNPSKIKMYDKTDGGTL
                     VLISGHTVPVSHRKKDYVMTLLENR"
     gene            complement(214..1521)
                     /gene="rimO"
                     /locus_tag="PK977_09330"
     CDS             complement(214..1521)
                     /gene="rimO"
                     /locus_tag="PK977_09330"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRF18360.1"
                     /translation="MKTKKLHKDKVNIITLGCSKNMVDSEVLSGQLKANDIDVVHENK
                     KLDHNIVVVNTCGFIDKAKEESINTILDQVELKRKGKLDKVYVTGCLSERYKNNLEAE
                     IPEVDAYFGTMELPLILKQFEADYKTELVGERLLATPQHYAYMKISEGCNRTCSFCAI
                     PLMRGQHVSKSIEDLVKEAEGLVKKGVKEVMLIAQELTYYGLDIYKKRMLGDLLHRLA
                     DVKGLEWIRLHYAYPSKFPLEVLDVMRERENICNYLDMPLQHAANNMLKAMKRQITRE
                     EMEELTIAIREKVPGICLRTTLIAGFPGETEDDVEELKGFLQRQRFDRVGIFTYSHEE
                     GTSAHELVDDVPAEEKERRAQEIMEVQQEISYELNQEKVGKIFKTIIDKKEAGRYLGR
                     TEFDSVEVDNEVVIHSSKKLPIGEFVNVKITKAYDYDLEGEVV"
     gene            complement(1521..2492)
                     /gene="ftsY"
                     /locus_tag="PK977_09335"
     CDS             complement(1521..2492)
                     /gene="ftsY"
                     /locus_tag="PK977_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRF18361.1"
                     /translation="MGFFNKLFGKKEKESLDEGLKKTKQGFFDKITKAIAGKSTVDEE
                     VLDNLEEALVSADVGIETTIKIIDRIEKRVAKDKYLNSSELNKMLQEEIETLLVDTPE
                     SNSYAFDSELPSTPYVILVVGVNGVGKTTTIGKLAYNFKKAGKEVLLGAADTFRAAAV
                     DQLTIWSERVGVPIVKQAMGSDPAAVAFDTVQSAVSKKSDVVLIDTAGRLHNKAHLMD
                     ELSKIKRVIQKTIPDAPHEVLLVLDGSTGQNALEQAKHFTAATDVTALAITKLDGTAK
                     GGVVLAIANQFKIPVKFIGVGEKMEDLLVFDKHEFVDSLFNLERKEA"
     gene            complement(2570..2743)
                     /locus_tag="PK977_09340"
     CDS             complement(2570..2743)
                     /locus_tag="PK977_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRF18362.1"
                     /translation="MAKAASKNAKVKDLKASAEAKNWTKVIKAVRSPKTGAYTFKEQI
                     IHKDKVKDFLAQK"
     gene            complement(2746..2928)
                     /gene="rpmG"
                     /locus_tag="PK977_09345"
     CDS             complement(2746..2928)
                     /gene="rpmG"
                     /locus_tag="PK977_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRF18363.1"
                     /translation="MAKKGNRVQVILECTEHKNSGQPGTSRYITVKNKKNNPERLELK
                     KFNPILKKVTVHKEIK"
     gene            complement(2949..3185)
                     /gene="rpmB"
                     /locus_tag="PK977_09350"
     CDS             complement(2949..3185)
                     /gene="rpmB"
                     /locus_tag="PK977_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRF18364.1"
                     /translation="MARVCQVTGKTPIGGHKVSHSNIKTKRRFLPNLQKKRFYLAEED
                     KWITLKLSTEAIRTINKNGLYSVVKQLRAKGEKI"
     assembly_gap    3386..3450
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3451..>3860)
                     /locus_tag="PK977_09355"
     CDS             complement(<3451..>3860)
                     /locus_tag="PK977_09355"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF18365.1"
                     /translation="TRGNVGSFHNLGATATLQLPVSKWWNLVSVAVYNYKIIKGVVWA
                     PIEARVHQLNISLNNQFQFKKGWAAELSGYYQTNSQIDLQESLTPQGEMGFGFSKQVL
                     KGSGSLRLTVRDLFYTQNYSGYSLFQNSDEPFRV"
     assembly_gap    3861..3870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3871..>4789)
                     /locus_tag="PK977_09360"
     CDS             complement(<3871..>4789)
                     /locus_tag="PK977_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF18366.1"
                     /translation="FTGSYLDRETKSYSTIDWMSPSYLIDSTINTWGTRHVDFSRSGV
                     NFNAKHVLQNKDEFTADVDFIRFDIKGGQFFQTQLNTPGSMVQATRGNVPSALEIFTA
                     KLDFSKRFKIYLWEAGVKTARTKTDNLADYFFNDGQNWYDDLSRSNHFLYDENISSVY
                     SSINADKEKWQWQVGLRYENTSYKANQLGNAVIKDSAFRKNYGSLFPSGFVSYKLDSN
                     HTLTFRLGRRIDRPQFQNLNPFIITINKYTFEAGNPYIRPQYTWNFELMHSYRQMLTT
                     SISYSYLKDYFSQIFIIDSNSSNVNKNTMI"
     assembly_gap    4790..4821
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4822..5676)
                     /locus_tag="PK977_09365"
     CDS             complement(<4822..5676)
                     /locus_tag="PK977_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18367.1"
                     /translation="MAGVTVQLLKADSSLIKVQITDKSGIAEFSNLPGNEYIVLATHL
                     GFQPAARDIKNLQQQPEINITITLQAGVATLADVVVSSRKPFVQFAPDKTVVNVDAGI
                     TNAGTSIMDVLEKSPGVTVGRDGTISMKGKAQVMVLIDGKQTQLSGADLQAYLTGISA
                     SQVDVIELIDNPGAKYDAAGNAGIINIRTKKLRQKGFNGSLSVGLGQGVYPKSNSSLN
                     LNYRNGKVNVFLNYGNRIGKEMMEMYALRKYFDKNGNDSLLLQQPNFTKTTIDAHNIK
                     TGFDFFASS"
     gene            5996..>6100
                     /locus_tag="PK977_09370"
     CDS             5996..>6100
                     /locus_tag="PK977_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF18368.1"
                     /translation="MAYDVVVLGSGPGGYVAAIRASQLGFKTAVIEKES"
     assembly_gap    6101..6114
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6115..6517
                     /locus_tag="PK977_09375"
     CDS             <6115..6517
                     /locus_tag="PK977_09375"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18369.1"
                     /translation="GICLNWGCIPTKALLKSAQVFEYIKHAKDFGIEASGTPQFDSVI
                     KRSRGVADKMSKGVQFLMKKNKIDVIMGFGKLVAKGKLEVTAQDGSKQTVEAKHIIIA
                     TGGRSRELPSMKIDGKKIIGKLWYCHKCPNQ"
     gene            6502..7401
                     /locus_tag="PK977_09380"
     CDS             6502..7401
                     /locus_tag="PK977_09380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18370.1"
                     /translation="MPKSMIIVGSGAIGVEFAYFYNSMGTKVTIVEFMPRIVPVEDED
                     ISKELEKQYKKQGISIMTSSEVTAVDTSGDGVKAKVKTATGEVTLEADVLLSAVGVAA
                     NIEGIGLETLGVKTDKGKILVDKYGQTNIPGVYAIGDCSPGQALAHVASKEGIICVEA
                     IAYNENKYHHQPEAIDYNNIPGCTYCVPEIASVGYTEKQAKEAGYEVKVGKFPLSASG
                     KASAAGHPEGFIKVIFDAKYGEWLGTHMIGYNVTEMIAETVTARKLETTYHEVLNAIH
                     PHPTISESVKDAIEVAYGEAIHI"
     gene            complement(7538..>8550)
                     /locus_tag="PK977_09385"
     CDS             complement(7538..>8550)
                     /locus_tag="PK977_09385"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF18371.1"
                     /translation="YPSEKISDEQVYSQIDALNKAFRRKNADTINTPAWFRPLAADCE
                     IEFQLAISDPRRRSTTGIIRKYTPIKSWEANDKMKFSSEMGSDAWDSKSYLNIWVCNL
                     ESVAGYSSVMGGPAEKDGIVIGFSAFGTNTGMTGYEMGKTAVHETGHWLGLKHLWGDE
                     YCGDDGITDTPRQAGNNVGCPTGIRGSCNNGPNGDMYMNYMDFTSDACMNLFTKGQKE
                     KMRALFASGGLRNPLLSSKGLQMPLIMESPLPEQDPKWLEVKMYPNPASTELYLDLAY
                     DTRWIGKNIFVTNLQGQHLMNVTITSKNQRIDISRLQPGIYFIAAKKDDGESIKMRFV
                     KL"
     assembly_gap    8551..8571
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8572..8795)
                     /locus_tag="PK977_09390"
     CDS             complement(<8572..8795)
                     /locus_tag="PK977_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18372.1"
                     /translation="MKAGLIGIIFILAALQLAAQKNCVSHSYQQQQFTYDPTLQDRNN
                     TVESFIRNQLIHANSLRIEGTETVIKIPVVV"
     gene            9094..>9456
                     /locus_tag="PK977_09395"
     CDS             9094..>9456
                     /locus_tag="PK977_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HRF18373.1"
                     /translation="MKKILFAVAVLGIIGCKNKDNDDPNQPDPNAPKGMSYSIVGTFP
                     HDTSSYTQGLQIYNGELYEGTGNYGFSKLKKVDLQTGKTLRETALDKKYFGEGITILN
                     DTVYQLTWKEKKVFVYTLN"
BASE COUNT         2568 a         2043 c         1835 g         2868 t
ORIGIN      
        1 aattgtaatc tctaaaaacc tgagtacata tgaagacctg ctgaattctt atggttttat
       61 cagaacacat cactcttacc ttgtaaaccc atccaaaatt aaaatgtatg ataaaacgga
      121 tggcggtaca ttggttctga tcagcggaca tacggtacct gtttctcacc gtaaaaaaga
      181 ttatgtaatg acactacttg aaaacaggta ggattaaacc acctcacctt caaggtcata
      241 gtcataggcc tttgtgattt ttacattcac aaactcacca atggggagtt ttttggaaga
      301 gtgtatcact acctcgttat ccacttctac agaatcaaat tcagtgcggc ccaggtagcg
      361 gccggcttcc ttcttatcaa ttatggtttt gaagatcttt cccacttttt cctggttcag
      421 ttcatatgaa atttcctgtt gcacttccat gatctcctgc gcccttctct ccttctcttc
      481 tgcgggtaca tcatctacca gttcatgtgc agaagtacct tcttcatggg agtaggtgaa
      541 tatgccgaca cgatcaaaac gctgacgctg taaaaaacct ttcagttctt ccacatcatc
      601 ttccgtttcg ccgggaaagc cggcaatcaa tgtggtgcgt aaacaaatgc cgggaacttt
      661 ttccctgata gcaatggtca attcctccat ctcttctctt gttatctgcc ttttcatggc
      721 tttcagcata ttgttggctg catgctgcaa aggcatatcc aggtaattgc agatattctc
      781 tctttcacgc atcacatcca gcacttccaa tggaaatttt gacgggtagg cataatgcaa
      841 acggatccac tccaggcctt ttacatcggc aagccggtgc agcaggtctc ccaacattct
      901 ctttttgtaa atatcaaggc cgtagtaagt taactcttga gctatcagca tcacctcctt
      961 tactcccttt tttacaaggc cctctgcttc ttttaccaaa tcctctatgc ttttgcttac
     1021 atgttgtccc ctcatcagcg ggatagcaca aaaagaacaa gtgcggttac agccttccga
     1081 tattttcatg taggcataat gctgaggagt agccagcaac ctttcaccta ccaattccgt
     1141 tttgtaatct gcctcaaatt gttttaatat caacggaagt tccatagtac cgaaataggc
     1201 atccacctcc ggtatttcag cttccaggtt gtttttgtag cgttcactca ggcaaccggt
     1261 gacatacact ttatccaact tccctttcct tttcagttca acctggtcca aaatggtatt
     1321 gatactttcc tcttttgcct tgtcaataaa cccacaggta ttcacaacta caatattgtg
     1381 gtccagcttt ttattttcat gcaccacgtc aatatcgttc gccttcaact ggccactcaa
     1441 tacctcactg tccaccatgt tcttgctgca acccagtgtg atgatgttga ccttgtcttt
     1501 atgcagtttt tttgttttca ctaagcttct tttctttcca ggttaaataa actgtcaaca
     1561 aactcatgct tgtcaaatac cagcaggtct tccatttttt ctcccacacc tatgaatttt
     1621 accggtatct taaactggtt ggctatcgcc agcacaactc cgccctttgc cgttccatca
     1681 agcttcgtaa tggcaagagc tgttacatct gttgcagcgg taaaatgttt agcctgttcc
     1741 agtgcatttt gcccggtact gccatccagc accagcaaca cttcatgtgg cgcatcgggt
     1801 attgtctttt gaattaccct tttgatcttg ctgagttcat ccatcaggtg ggctttgttg
     1861 tgcagccttc cggccgtatc aatcagtacc acatcggatt ttttggaaac agcgctttgc
     1921 actgtatcaa atgccacagc ggccgggtca ctacccattg cctgttttac aatgggaacg
     1981 ccaacccttt cactccaaat ggtcagttga tccacagcag ctgccctaaa tgtatctgca
     2041 gcacccagta aaacttcttt acctgccttt ttaaaattat aagccagttt gccaattgta
     2101 gttgtttttc ccacaccatt tacacctacc accaatatga cataaggcgt ggaggggagt
     2161 tcagaatcaa aagcataact atttgattca ggggtatcca ccaacaaagt ctcaatttct
     2221 tcctgcagca tcttgttcag ctcagatgaa ttaagatact tgtctttggc cacccttttt
     2281 tcaatccggt cgatgatctt tatggtcgtt tcaatgccaa catcggcact tacaagggct
     2341 tcctccaggt tgtccagcac ttcttcatct acggtgcttt taccggcaat ggccttggtt
     2401 attttatcaa aaaagccttg tttggttttc ttcaaacctt catccaggct ttctttttcc
     2461 tttttaccga acagcttatt aaaaaatccc atttctgaca attttcatta aacaacaaaa
     2521 gccatccgtt ttttacgaat agcttttgaa tacattttaa atagctcaat tatttctggg
     2581 ccaggaaatc cttcaccttg tctttgtgga tgatctgctc tttgaaagtg taagcaccgg
     2641 tcttagggct gcgtacagct ttgatcacct tagtccagtt tttagcctca gcagaggcct
     2701 taaggtcttt tactttagcg tttttagaag ctgcttttgc catgattatt taatttcttt
     2761 atgaacagtt acttttttca gaatcgggtt aaacttcttc aactccagac gctccgggtt
     2821 gttctttttg ttttttaccg tgatataacg gctggtgccg ggctgaccac tgtttttgtg
     2881 ttcagtgcat tccaggatta cttgtacacg gttgcctttc tttgccatga ttattaatct
     2941 ttaaaaagtt agattttttc tccttttgcc ctcaactgct tcactacgct atataaacca
     3001 ttcttgttga tggtacggat agcttcagta gatagcttca gggtgatcca tttatcctct
     3061 tcggccaggt agaacctctt tttctgcaaa ttgggcagaa aacgacgctt ggtcttgata
     3121 ttggagtgag aaaccttatg tcctccgatc ggcgttttac ctgtaacctg acatactctt
     3181 gccatgattg atacaatttt tgggacggca aaggtaaggg aaagatgcca gaattttgcg
     3241 cctaaactaa gtttttttta accgcttgtt gaaaacctct gatctcactt ttgtcctgat
     3301 tttcaaaggc aatgaatgat taattacctg ctcctaccct ctctgtctct tccgcggcac
     3361 ctccggcaga tcgcctgatg ggtttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caccctgaat ggctcgtccg aattttggaa
     3481 caaggaataa cccgaataat tctgagtata gaaaaggtcc ctgacagtaa gccgaagtga
     3541 cccactgccc ttcaaaacct gctttgaaaa accaaagccc atttctccct gtggagtcag
     3601 gctttcctgt aaatctattt gactattggt ctggtaataa ccactcaatt cagcagccca
     3661 acctttttta aactggaact ggttattgag actgatattt aactggtgaa cccttgcctc
     3721 aataggagcc catactaccc cttttattat tttgtaatta tagacagcta cgctgacaag
     3781 gttccaccat tttgaaaccg gcagttgcaa ggtggcagta gcaccgaggt tatgaaaact
     3841 cccgacatta ccccgggtgt nnnnnnnnnn gatcatggta tttttattta cattactgct
     3901 gtttgaatca atgataaata tctgggagaa ataatctttg agatagctgt aagaaatgct
     3961 ggtagttagc atttgccggt aactgtgcat taactcaaaa ttccaggtgt attggggtct
     4021 tatataggga ttaccagctt caaatgtgta cttattgatg gtgataataa aaggattcag
     4081 gttctgaaac tgaggccgat caattcggcg gcccaatcga aatgtaagtg tatgattact
     4141 gtcaagttta tagctaacaa atcctgacgg gaaaaggcta ccgtaatttt tccggaaagc
     4201 tgaatctttt attactgcat ttcctaactg attagccttg tagcttgtat tttcataacg
     4261 aagccccacc tgccattgcc atttctcttt atcggcattt atactgctgt agactgaact
     4321 gatattctca tcatataaaa aatgattact cctgctcaga tcatcgtacc agttttgccc
     4381 atcgttgaag aaataatccg ccaggttatc tgttttcgta cgagccgtct tcactccagc
     4441 ttcccataaa tagattttga acctttttga aaaatccagt tttgccgtaa aaatttcaag
     4501 tgcagatggt acattacccc gtgtggcctg aaccatacta ccgggtgtgt ttaattgagt
     4561 ttgaaaaaac tgtccgcctt taatatcaaa ccggataaaa tcaacatcag cggtaaattc
     4621 atccttgttt tgtaaaacat gttttgcatt gaaattaact cctgaacggc tgaaatcaac
     4681 atgacgggtg ccccaggtat taatagtgga atcgataaga taagatggcg acatccagtc
     4741 aatagtacta tagctttttg tctccctgtc aagatatgag cctgtgaatn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nggatgatgc aaaaaaatcg aatcctgttt ttatattatg
     4861 tgcatcaatg gtggttttgg taaaattggg ctgttgtagg agtaacgaat cattaccatt
     4921 tttgtcgaaa tatttccgaa gggcatacat ttccatcatt tctttaccaa tccggttgcc
     4981 ataattcaga aatacattca cttttccatt ccggtaattc aggttcaggc tgctgttcga
     5041 ttttggataa accccctgtc ccagccccac actcaaggaa ccattaaagc ccttttgcct
     5101 tagctttttt gtccggatat taatgatccc ggcgttacca gctgcatcgt atttggcacc
     5161 cggattatcg atcagctcaa taacatccac ctgtgaagcg ctaatgcctg taagataggc
     5221 ctgcaggtca gccccgctaa gttgtgtctg tttgccatcg atcaatacca tgacctgcgc
     5281 cttacccttc atactgatgg tcccatctct gcctactgtt acaccgggag atttttccaa
     5341 aacatccata atggaagtgc ctgcattcgt aatacctgca tctacattca ctacggtttt
     5401 atccggagca aactgtacaa atggtttcct tgaacttact accacatccg ccagcgtagc
     5461 aaccccggct tgcaacgtaa tggtaatatt aatttcaggc tgctgttgca ggttttttat
     5521 atctctggcg gctggctgaa aaccaagatg ggtggccaaa acgatgtact cattaccagg
     5581 gagattagaa aattcagcga tacccgattt atcagttatc tgtactttta ttaatgaaga
     5641 atcagccttt agcaattgaa ctgttacacc cgccaacggc tcttgtgcgt catttacaat
     5701 aagtacctta acaggttttg aaaatgactt ttctgcgaaa caggaggttt ggcattgaaa
     5761 gaaacagata ataaaaacaa atagcattct taataacatg ctccgaaaat aaacgacttc
     5821 cttaaataga ttataaaaat gggtttagcg gttttatttg aagatagatg aggttttcgt
     5881 ttaactgctt aacactggtg acgttggcct tggccgtttt gcccgtttgg gggttttcaa
     5941 ataacttcgc accgcttttg tactacaatt ttataaatca actaatacac taaatatggc
     6001 atacgatgta gtggttcttg gtagcggtcc cggtggttat gtggctgcta tccgtgcatc
     6061 ccaattagga tttaaaacag cagttattga aaaagaaagc nnnnnnnnnn nnnntggcat
     6121 ctgcctcaac tggggttgta tcccgaccaa agcgctttta aaaagtgccc aggtttttga
     6181 atatataaaa catgcaaaag actttggaat tgaagccagc ggtacgccac agtttgattc
     6241 agtgattaaa agaagccgtg gtgtagctga taaaatgagc aaaggcgttc agttcctgat
     6301 gaagaaaaac aagatcgatg tgataatggg ctttggtaaa ctggtggcta aaggcaaact
     6361 ggaagtaact gcccaggatg gcagcaagca aactgtggaa gccaaacata ttatcattgc
     6421 taccggtggc cgcagccgtg aactgccgag tatgaaaatt gacggaaaga agatcatcgg
     6481 gaagctatgg tactgccaca aatgcccaaa tcaatgatca ttgtaggtag cggtgccata
     6541 ggtgttgaat ttgcctactt ctataacagc atgggaacca aagtaacgat cgttgagttc
     6601 atgccacgca ttgtacccgt ggaagatgag gatatttcaa aagaactgga aaaacaatac
     6661 aaaaagcaag gtattagtat catgacaagc agcgaggtta ccgctgttga taccagcgga
     6721 gatggggtga aagcaaaagt aaaaacagct accggtgagg tcacactgga agctgatgta
     6781 ttgttgagtg cagtaggtgt agcagccaat atcgaaggaa ttggactgga aacgctgggt
     6841 gtaaaaacgg ataaaggaaa aattctggtt gacaagtatg gtcagactaa tattcccggt
     6901 gtatatgcca tcggtgactg ttcacctggc caggctttag cgcatgtggc cagcaaagaa
     6961 ggtattatat gtgttgaagc tatcgcttac aacgagaaca aataccatca tcagccggaa
     7021 gcgattgatt ataataatat tccaggttgt acttactgtg ttccggaaat agcttctgta
     7081 gggtatactg aaaaacaagc taaagaagcc ggctatgaag taaaagtcgg caagttccca
     7141 ctaagtgcca gtggtaaagc ttctgcagcc ggtcatccgg aaggcttcat caaagtaatc
     7201 tttgatgcca agtacggcga atggttaggc actcatatga tcggttacaa tgtaacagaa
     7261 atgattgccg aaacagtaac agcccgcaag ctggaaacaa cttaccatga agtcctgaat
     7321 gccattcacc cccacccaac cataagtgag agtgtgaaag atgcgattga agtggcatac
     7381 ggcgaagcaa tacatattta ataaataatt gcatccaaag gaataaaagg attatcgtta
     7441 acccggtaat ccttttttga ttattagagg taattgaaaa gccccggatt tctccggggc
     7501 catttgcttg ccgttagtat tattttggtt gcggccacta cagttttaca aacctcatct
     7561 taatagactc tccgtcatct tttttcgctg caatgaaata aataccgggc tgcaggcggc
     7621 taatgtcaat acgttgattc tttgacgtaa ttgtcacatt cataaggtgt tgcccctgca
     7681 ggttagtcac aaaaatattt tttccgatcc agcgggtatc ataggctaga tccaggtata
     7741 attcagttga agcaggattg ggatacattt tcacttccag ccattttgga tcctgctcag
     7801 gaagcggaga ctccattatt aatggcattt gcaacccttt cgaactaagc aatggatttc
     7861 ttaaccctcc ggacgcaaat aatgctctca ttttttcttt ctgacctttt gtaaataagt
     7921 tcatacaagc gtcactggta aaatccatgt agttcatgta catgtcgcca ttcggtccat
     7981 tattgcatga gcccctgatg cctgtcgggc agccaacatt attaccagct tgtctgggtg
     8041 tgtcagtaat tccgtcatca ccacaatatt catcacccca aaggtgcttc agaccaagcc
     8101 aatgtcctgt ttcatgaaca gccgttttgc ccatttcgta acctgtcatt ccggtgtttg
     8161 tgccaaaagc tgaaaatcct atgacaatgc catccttttc tgcaggacca cccatcacac
     8221 tggaatatcc ggcaactgat tcaagattgc atacccagat atttaaatag cttttactgt
     8281 cccatgcatc actacccatc tctgaggaaa acttcatttt gtcattagct tcccaacttt
     8341 ttatgggggt gtacttacga ataataccag ttgtagacct ccgccttggg tctgaaatgg
     8401 ctaattggaa ttcaatttca caatcagctg ctaaaggcct gaaccaggcc ggggtgttga
     8461 ttgtgtcagc attttttctg cgaaatgcct tattcaaagc atcaatctgg ctgtaaacct
     8521 gctcatcact tattttctcc gatggataat nnnnnnnnnn nnnnnnnnnn nacaacaaca
     8581 ggaattttaa ttaccgtttc agttccttca attcgtaaac tgtttgcatg tattaattga
     8641 ttcctgataa aagactcgac tgtattattc cggtcttgta atgtcggatc atatgtgaat
     8701 tgttgctgct gatagctgtg agaaacacag tttttttgtg cagccagttg cagtgctgcc
     8761 aggatgaaga taatgccaat taatcccgcc ttcatagttt ccaagtagtt tgtggttacg
     8821 gcatggcata aattttttag tgccatgagt tgttcatgtg gaaatggaaa taataaagga
     8881 gtattgggaa atttataaga ggcctctcag ggatacgatt ttgaaggaga attttccggt
     8941 ataaggacga atgaagatca aatataatct attaaatgaa ttgtgcaaga agtttttgta
     9001 tcgtattttt ccgattctgg cctattaatg caaattcttt aacagcaggg ataaaagccg
     9061 ccaattaatt ttgtgatttt ataagtgagt tatatgaaaa agatactttt tgcagttgca
     9121 gtacttggaa ttattggttg caaaaacaaa gacaatgatg atcccaatca acccgacccc
     9181 aatgccccca aaggcatgag ctactccatt gtgggcactt ttccgcatga tacatcttct
     9241 tatacgcaag gcttacagat atacaatggt gaattgtatg aaggcaccgg taattatggc
     9301 ttctcaaagc taaaaaaggt tgatctccaa acaggcaaga cactccggga aacagcactt
     9361 gacaaaaaat attttggtga gggaattaca attttaaatg atacggttta ccaactgaca
     9421 tggaaagaaa agaaagtgtt tgtttataca ctgaat
//
LOCUS       DAORLS010000107         9421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3934_length_9421_cov_1.693359, whole genome shotgun sequence.
ACCESSION   DAORLS010000107 DAORLS010000000
VERSION     DAORLS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3934_length_9421_cov_1.693359"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>674
                     /gene="ccsA"
                     /locus_tag="PK977_09400"
     CDS             19..>674
                     /gene="ccsA"
                     /locus_tag="PK977_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRF18374.1"
                     /translation="MVIHPPVLFLGFATTIVPFAFAIAGLWKKDFGEWTKQTLPWSLF
                     SAAVLGLGIMMGAIWAYESLSFGGYWAWDPVENASLVPWMILISGIHTLLIYKHTGNA
                     LRASYLFMILSFGFILYSTFLTRSGILGEASVHAFTDLGMNWQLLTFLLAFVIPALVL
                     FAIRYKEIPAIQKEEHTSSREFWMFIGSLIFFLSAILIIGQTSLPVINKVFGTNIAPP
                     "
     assembly_gap    675..688
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <689..1845
                     /locus_tag="PK977_09405"
     CDS             <689..1845
                     /locus_tag="PK977_09405"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRF18375.1"
                     /translation="IQVYVAIIMALLTAISQYFKYRRTDSKFLWKKILIPTILAVVAA
                     AAFILFGSINYLKKGPVFTAALWLAAICSMYSIIANAGYIWLGLKGRLKLSGGSIAHV
                     GFGMVLLGIVISASNKEVLTNNRNGIPAPLGDGENPMENLTLVQGLTVDMGKYNLTYE
                     GDSTHPKKPLSFYKIRFKSKDGKEEFVLKPNSFVNYKGNEGLMSNPASRHYWDHDVFT
                     YITSISNPDAAKDTTSFRTNKLKQGDTLFYSSGFLILQEVKKKDSLPEELFGKEGSLY
                     EAPIKVYSKTGSVYSVTSRLANAKGEALALPDTITAESLVLQLQNVYPDNSIDLGVKE
                     SNAVMKYVTIKAYRFPFILMLWLGVLVTTIGIIISMVRRMQLNRSGEATS"
     gene            1912..2622
                     /locus_tag="PK977_09410"
     CDS             1912..2622
                     /locus_tag="PK977_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRF18376.1"
                     /translation="MKSFKKYWRPALLVLLVFVVENKKAIYCQVYTDSTYKDSTYYAI
                     LAMKEQVKKWGPNDTIIVPAIFYQNEITNYKEMEMAWVSKLSPDKLAKFIEEWTRLRN
                     AVYVTYPYARIAGATINDINKNLEKVSNKKERKAYIKSRETELRKQFEEPLTNLSVYQ
                     GKVLMKLINRQTGNNCYEIIKEYKGGLNARVYQTVAWFVGGNLKQDWNLAKNKMDRQI
                     ESIVKEIDGGWYNNPYRR"
     gene            2672..3409
                     /gene="bshB1"
                     /locus_tag="PK977_09415"
     CDS             2672..3409
                     /gene="bshB1"
                     /locus_tag="PK977_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HRF18377.1"
                     /translation="MKVDILAIGVHPDDVELGCSGTIINEIKWGKKVGIIDLTEGELG
                     TRGTVQTRYQEAAAAAMIMGVHARENLQMRDGFFENDEAHRLKLITAIRKYQPDIIIG
                     NVLHDRHPDHGRAGKMIADAAFLAGLSKIVTKDEYGNDQPRWRPSYVLHYIQDWYHEP
                     DLLIDISDVFDQRMKSIEAYSTQFHTSEAGGEGPQTYISTPDFLESVIARARMFGKRI
                     GVKYAEGFISEKKIGIRNLDALIQNET"
     gene            3538..>3790
                     /locus_tag="PK977_09420"
     CDS             3538..>3790
                     /locus_tag="PK977_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRF18378.1"
                     /translation="MASPKFSHTSQSFHTELKKRINDYFKQAGKSSTGNFNLYLKAVL
                     LVIAYLFVYIHLVFYTPATWLALVECIILGGLTAAIGFNI"
     assembly_gap    3791..3811
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3812..>4265
                     /locus_tag="PK977_09425"
     CDS             <3812..>4265
                     /locus_tag="PK977_09425"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF18379.1"
                     /translation="NIMHDGMHGSFSRYKWVNNLAGLSLNFLGANNFMWKTKHNIIHH
                     TYTNVEGIDDDIEAKPLLRLCETQKYYKIHRFQHWYFWAAYSLLYIWWVFVTDYKKYF
                     LKRIGPTPLKPMSLLDHISFWGFKALHAFLFVALPIYMVGFIPWLIGFL"
     assembly_gap    4266..4301
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4302..4640
                     /locus_tag="PK977_09430"
     CDS             <4302..4640
                     /locus_tag="PK977_09430"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF18380.1"
                     /translation="SIVFQLAHTVEHTHFPQANPETHKMEDEWAIHQLKTTANFATSN
                     KLISWWVGGLNFQIEHHLFPKISHVHYPAISKIIKKACKDFNIPYIEYPKMRLAVASH
                     VSHLKNLSRK"
     gene            4761..5804
                     /locus_tag="PK977_09435"
     CDS             4761..5804
                     /locus_tag="PK977_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRF18381.1"
                     /translation="MIDIANYDPNSVGNPNNNIFGLPSTEDNSLLVILPVPWEVTVSY
                     AAGTARAPEAILKASLQVDLFDPEMPDAWKQGFYMRPVDKKILMKSDYLRKEAELYID
                     YISKGEEVQANQFMCKTLKDVNEGSSFMNNWVYEQTKALLDKGKLVALLGGDHSTPLG
                     FFKAMAEKHGEFGILQIDAHCDLRTAYEGFNYSHASIMYNALNEIKNLQRIVQLGVRD
                     YSCDEWEYIRNSNFRVVTYFDKEIKHRMFEGQTWKQIADEIVSKLPEKVHISFDVDGL
                     DPKLCPHTGTPVHGGFETEQVFYLFSKIQQSGKKIIGFDLVETGVSESDWDANVGARI
                     LFKLCNLLVTSNS"
     gene            5806..>6043
                     /locus_tag="PK977_09440"
     CDS             5806..>6043
                     /locus_tag="PK977_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18382.1"
                     /translation="MQEFVLILKHDKIVSYKRSILAIIGLNIIYFVAQAIITNETMAV
                     NRCLFLVIISFSLFITEYLLSKKKKKIFSAAIVVC"
     assembly_gap    6044..6131
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6132..6382
                     /locus_tag="PK977_09445"
     CDS             <6132..6382
                     /locus_tag="PK977_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18383.1"
                     /translation="ILLLYLFAIQVPIVHISADGIKLNILGKRFIKWEQLNNLVLKDG
                     LVTIDLKNNKLIQQYLDENSMPVNEKEFNDFCKKQLNK"
     gene            6379..>6560
                     /locus_tag="PK977_09450"
     CDS             6379..>6560
                     /locus_tag="PK977_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF18384.1"
                     /translation="MSLLQSPYILYSDGKGNIFEDTSLYVTGRSGWDAFEVPADEWIE
                     LPDGGSLYELPGRRGIG"
     assembly_gap    6561..6579
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6580..>7388
                     /locus_tag="PK977_09455"
     CDS             <6580..>7388
                     /locus_tag="PK977_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF18385.1"
                     /translation="TGEMRLCEKGWAVAAFIPPAHTGFYLAAYETAAEAPTLPLFCYT
                     AAGWHNDKFYVPATRIEPDIRQDCAGYDQQKVNQGAAYLLKHYPNNRLVDHLMNNCAL
                     TNQCPAARNFALGRWECPVPASPACNANCVGCISLQPDEEPIVSTQDRLTFKPTPEEI
                     VEFTVPHLETAPYPIISFGQGCEGEPLLMWETIRDAIIEIRKHTKKGSININTNGSRP
                     DAVDELCKVGLDSIRVSTNSARREIYMPYYRPNNYDFDDIVESLKVVNRYGG"
     assembly_gap    7389..7453
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7663..7674
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7833..8708
                     /locus_tag="PK977_09460"
     CDS             7833..8708
                     /locus_tag="PK977_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18386.1"
                     /translation="MNNAYAMSASLEARNRSQAYMITAGFTGLMLLLMFLLKWDMPSF
                     ERTVQEPGIEVELNLPEEPETLADGGGGGGNPVQAAGDPGIAPYTPPAPGEEQDSKDI
                     EEDNDKDAPEVTKPVVAKPKATTITNTSVAKTEPKPVVETPAPRVPKAVLGKTTTGTG
                     RGGGAEDYDRAGGSGTGSGVGNGSGTGGGSGSGNGGGNGSGTGSGNGPKVTRGDRKIV
                     RSYSFEGDLDRATIYANVNVSPEGVGTFVSIAKGSSNSGSAYKNAIIQYLQRIRFDKS
                     DHESMITVQFNFRVN"
     gene            8747..>9045
                     /locus_tag="PK977_09465"
     CDS             8747..>9045
                     /locus_tag="PK977_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18387.1"
                     /translation="MASLFIFLYNMNYQETIDYLFTRLPMFSRIGAAAYKKDLTNTVE
                     LCTFLGNPQKKFKSIHIAGTNGKGSVSHMLAAILQTAGYKTGLYTSPHLKDFRER"
     assembly_gap    9046..9136
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9156..>9421
                     /locus_tag="PK977_09470"
     CDS             9156..>9421
                     /locus_tag="PK977_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF18388.1"
                     /translation="MAFDYFAHEEVDIAVIETGLGGRLDSTNVIFPVLSVITNIGWDH
                     MNMLGDTLEKIAFEKAGIIKEGVPVVIGEVLAETLPVFENAAKE"
BASE COUNT         2779 a         1798 c         1934 g         2564 t
ORIGIN      
        1 cttttgcaaa attactggat ggtcatccac cctcctgttt tgtttcttgg ttttgctact
       61 actattgttc catttgcatt tgccattgcc ggattatgga aaaaagattt tggcgaatgg
      121 acaaaacaaa cgctgccatg gtcgctattt tccgcagccg ttttgggatt gggcatcatg
      181 atgggagcaa tctgggctta tgagtcgtta agctttggtg gttattgggc atgggatcct
      241 gtagagaacg cctcactggt tccctggatg attctaattt caggtatcca tacgctgctg
      301 atctataagc atacaggtaa tgcgttaagg gcctcctacc tgtttatgat actttcattt
      361 ggattcattt tgtattcgac cttccttaca agaagcggta tactgggtga agcctctgta
      421 catgctttta cagatttggg catgaactgg cagctactga catttttact ggcatttgta
      481 attccggccc tggtgctttt tgccattcgg tataaagaaa ttcccgccat tcaaaaagaa
      541 gaacatactt cctcaagaga attctggatg ttcattggct ctctaatttt cttcttatct
      601 gccattctta taatcggcca gacctcgttg cctgttatca ataaagtatt tggcaccaat
      661 atagcccctc cggannnnnn nnnnnnnngc atacaggtat atgtagctat tatcatggca
      721 ttattaaccg ctatttcaca atattttaaa tacaggcgaa cggatagtaa atttttatgg
      781 aaaaaaatat taatcccaac catcctggct gttgttgctg cagctgcttt tatcctattc
      841 ggcagcatca attatttaaa aaagggccct gttttcacag ctgcgttatg gctggcagcc
      901 atttgcagta tgtacagtat tattgccaat gccggatata tttggctggg tttgaaagga
      961 aggcttaagt tgtccggagg ctctattgcc catgtgggat ttgggatggt attattgggc
     1021 attgtcattt ctgcttctaa taaagaggta cttaccaata acaggaatgg tattcccgcc
     1081 cctctcggtg atggtgaaaa cccgatggaa aatcttacgc tggttcaagg attaactgtc
     1141 gatatgggta aatataatct tacttatgaa ggagattcca cacaccccaa aaagccactt
     1201 tccttttaca agattcgttt caaaagcaag gatggcaagg aagaatttgt actgaaaccc
     1261 aattcctttg taaattataa aggaaatgag ggcctgatgt caaatcctgc atcacggcat
     1321 tattgggatc atgatgtatt cacctatatc acttctattt ctaacccgga tgctgcaaag
     1381 gataccacta gtttcaggac taataaactg aaacagggcg ataccctttt ctattcatcc
     1441 ggctttttga ttttacagga ggtaaagaaa aaagacagcc ttccggaaga gttatttgga
     1501 aaagaaggaa gcctttacga ggcacccatt aaagtttatt caaaaacagg atctgtttat
     1561 tcagttactt caagactggc caatgcaaaa ggagaggcgt tggccttgcc tgataccatc
     1621 acagctgaaa gccttgtact gcaattacag aatgtgtacc cggacaacag tattgattta
     1681 ggtgtgaagg agtccaatgc agtaatgaag tatgttacca tcaaagcata ccgctttccg
     1741 tttatactaa tgctttggct cggtgtgttg gtaaccacta ttggaatcat catcagtatg
     1801 gtacgccgga tgcagttaaa ccgttccggg gaagcaacgt cctaattttg gcttttttaa
     1861 aacagcatct tgtgtagctt tactgtctgt tttagaatgc ctgtacggta aatgaagtct
     1921 ttcaaaaaat actggcggcc tgcattgctg gttttgcttg ttttcgttgt ggaaaacaaa
     1981 aaggcaattt attgccaggt atacaccgac tctacctaca aagattccac ctattacgcc
     2041 atcctggcta tgaaagagca ggtaaaaaaa tggggaccaa acgatacgat cattgtaccg
     2101 gcaatttttt accaaaacga aatcactaac tataaggaga tggaaatggc ctgggtgtca
     2161 aaactatctc ctgataaact ggctaagttt attgaagaat ggacaagact tagaaatgcg
     2221 gtttatgtaa cctatcctta tgcaaggatt gcaggcgcca ctatcaatga catcaataaa
     2281 aacctggaaa aagtaagcaa taaaaaagaa aggaaagctt atataaagag cagggaaaca
     2341 gagcttcgaa aacaatttga agaaccattg accaatcttt cagtttacca gggaaaagta
     2401 ctgatgaaac tgattaaccg ccagactggt aataactgtt acgagatcat taaagaatat
     2461 aaaggcggat taaatgcaag ggtgtaccag acagtagcct ggtttgtagg tggtaacctg
     2521 aaacaggact ggaaccttgc caaaaataaa atggaccgcc agatagaatc tattgtaaaa
     2581 gaaattgacg gtggctggta taataatcct tatcgccgct aattacttca cttgcttttt
     2641 tccatttact tttgatactt aaacagctga catgaaagtt gatattttag ccattggtgt
     2701 tcacccggat gatgtggaat tgggctgctc cggtaccatt atcaatgaga taaaatgggg
     2761 aaaaaaagtc gggatcattg acctcacaga aggcgaactg ggcacaaggg gaacagtaca
     2821 aacccgctac caggaagcag cagcagcggc aatgattatg ggagttcatg ccagggaaaa
     2881 cttgcaaatg agagatggat tcttcgaaaa cgatgaggct caccgtttaa aattaattac
     2941 cgcaatccgt aaataccagc ctgatatcat catcggtaac gtattacatg atcgtcaccc
     3001 ggaccatggc agagccggaa aaatgatcgc cgatgcagca tttcttgccg gtctttccaa
     3061 aattgttaca aaagatgaat atggtaatga ccagccaaga tggcgacctt cttatgtact
     3121 tcattatatt caggactggt atcatgaacc agacctgctt attgacatca gtgatgtttt
     3181 tgaccaaaga atgaaatcaa ttgaagctta ttccacacag tttcatacca gcgaagcggg
     3241 aggtgagggg cctcaaacct atatttccac tcctgatttc ctggaaagtg tcatagcaag
     3301 agcccggatg tttggcaaac gtattggcgt aaaatatgca gaaggattta tatctgagaa
     3361 gaaaattggc atacgcaacc tcgatgcgtt gattcaaaat gaaacctgag caatcgtttg
     3421 attaatgttt tcttgatccc ggttaaacaa atcaggccac tttgccaaat caaattgagt
     3481 tttaaccaat ttattttccg gatttgattt atctttacca atcactaatc tctgataatg
     3541 gcatctccga aattctcaca cacttcgcag tcttttcaca ctgaattaaa gaaaaggatt
     3601 aacgactatt tcaagcaggc tggcaaatct tccacaggta attttaattt gtacctgaaa
     3661 gcagttttgc tggtaattgc ctatttgttt gtttacatcc acctggtatt ttataccccc
     3721 gcaacctggc ttgcactggt tgaatgtatt attttagggg gacttaccgc agcaattggc
     3781 tttaatatca nnnnnnnnnn nnnnnnnnnn naatatcatg catgatggca tgcacggtag
     3841 tttcagccgt tacaaatggg taaataacct ggccgggctg tctttaaatt ttttaggggc
     3901 aaataacttc atgtggaaga ccaagcataa tatcattcac cacacctaca caaacgttga
     3961 aggtattgat gatgatattg aagcaaaacc gttattacgt ctctgcgaaa cacaaaaata
     4021 ttataagatc caccgctttc agcactggta tttctgggcc gcatattccc ttttatatat
     4081 atggtgggtg tttgtaacag attataaaaa atattttttg aagcgtattg ggcctactcc
     4141 attaaaacca atgagtctac tggaccatat ttcgttttgg ggttttaaag ctttacatgc
     4201 atttcttttt gtagctctgc ccatatatat ggtcggtttt ataccctggc ttataggatt
     4261 tcttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtattgta tttcagttgg
     4321 cacatacggt ggaacatact cattttcctc aggcaaatcc agagactcat aaaatggaag
     4381 atgaatgggc tattcatcaa ttgaagacaa cagccaattt tgccacaagt aataaactta
     4441 tttcctggtg ggtaggcgga ctcaatttcc agattgagca ccacctgttt cccaaaatat
     4501 cccatgtaca ttaccctgct atcagcaaga tcattaaaaa ggcatgcaag gatttcaata
     4561 ttccttatat agaataccca aaaatgagac tagcagtagc ctcacatgtg tctcacctga
     4621 aaaatctgag tcgcaaataa aagaattgct agataaacga ataaataggc aaggacttat
     4681 ccttgctttt tttatttgca tcagtatatt ttgccgctat ggtaaacggg tttaacttgc
     4741 acagctttta atacttcaaa atgattgata tagccaatta tgacccgaat agtgtaggca
     4801 atcctaacaa caatattttt ggattgcctt caacagaaga caactccctc cttgtcatat
     4861 taccagtgcc atgggaggta acagtaagct atgctgccgg cacggcaagg gccccggagg
     4921 ccattctgaa agctagccta caggtggatc tttttgaccc cgaaatgcca gacgcctgga
     4981 aacaaggctt ttatatgcgt ccagttgata agaagatcct gatgaaaagc gactatctgc
     5041 gaaaagaagc ggaattatac atcgattata tttcaaaagg cgaagaagta caggctaacc
     5101 agtttatgtg caaaaccctc aaagatgtaa atgagggaag ttcttttatg aacaactggg
     5161 tgtatgaaca aacaaaagcg ctacttgaca aaggcaaact cgttgcactc ttaggaggtg
     5221 accatagtac acctctgggc tttttcaaag ccatggcaga aaaacatggt gaatttggta
     5281 tactgcagat tgatgcccac tgtgacctcc gaacagcata cgaaggtttt aactattccc
     5341 atgcaagcat aatgtataat gcccttaatg aaattaaaaa tttacagcgc atcgttcagc
     5401 tgggtgtaag agattacagc tgtgatgaat gggaatatat cagaaacagc aatttcaggg
     5461 tagttaccta tttcgacaaa gagataaaac atcgtatgtt cgaaggacag acctggaaac
     5521 agattgccga tgagatcgtc agtaaacttc ccgaaaaagt ccatatcagt tttgacgtag
     5581 atggtctgga tcctaaatta tgcccgcata caggtacccc tgtacatggt ggatttgaaa
     5641 cggaacaagt attttatctt ttctccaaaa tacagcaaag tggcaagaag atcattggct
     5701 ttgaccttgt agaaaccggc gtatcagaat ccgactggga tgccaatgtg ggcgcccgga
     5761 ttctatttaa actttgcaat ctgctggtaa caagcaatag ttaacatgca ggaatttgta
     5821 ctgatcttaa agcatgataa aatcgtctct tataaaagat cgatactggc aattatcggt
     5881 ttgaatatta tctatttcgt tgcacaagca ataattacca atgaaacaat ggcagtcaac
     5941 agatgccttt tccttgttat catttctttt tctcttttta ttaccgaata cctgctgagc
     6001 aaaaaaaaga agaaaatttt cagtgctgca atcgtcgttt gtgnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn ntaattttgc ttctctattt gtttgccata caggttccca ttgtacatat
     6181 ttcagctgac ggtataaaac ttaacatttt gggaaagcgg tttataaaat gggagcaatt
     6241 aaacaacctg gtattaaaag acggattagt gacgattgat ctaaaaaaca ataaactgat
     6301 ccagcaatac cttgatgaaa attcaatgcc tgttaatgaa aaagaattta acgacttttg
     6361 caaaaagcaa ctgaacaaat gagcctttta caatctccct atatactcta ttccgatggc
     6421 aaaggcaata tttttgaaga cacatcattg tatgtaacag gtagaagtgg ttgggatgct
     6481 tttgaagttc cggctgatga atggattgaa ttaccggatg gtggttcttt gtacgaactg
     6541 cccggcagaa gaggtatcgg nnnnnnnnnn nnnnnnnnna ccggtgaaat gcgtctttgt
     6601 gaaaaaggct gggctgtggc agcttttatt cccccggcac ataccgggtt ttacctggct
     6661 gcttatgaaa cggcagcgga ggctcctacc cttcctttgt tttgctatac tgctgccggc
     6721 tggcataacg ataaatttta tgtgcctgca acaaggatag aaccagatat aaggcaagat
     6781 tgtgctggct atgaccagca aaaagtaaat caaggggctg cttacctgct aaagcattac
     6841 cccaataaca ggctggtgga tcacctgatg aacaactgtg cattaaccaa tcagtgtccg
     6901 gccgcaagaa actttgcatt aggcagatgg gaatgccctg taccggcctc tccggcctgc
     6961 aatgcgaatt gtgttgggtg tatatcactg cagccggacg aagaacctat tgtttctaca
     7021 caggacaggc tgacttttaa gcctacacca gaagaaattg tggagttcac ggttccccat
     7081 cttgaaacag caccctaccc tatcattagt tttggccagg gctgtgaagg tgagccgctg
     7141 cttatgtggg agaccattcg tgatgctata attgaaataa gaaaacatac taaaaaaggg
     7201 agtattaata tcaacaccaa tggatcaaga cctgatgctg tagatgaact atgcaaagtc
     7261 gggttggatt ctataagggt gagcaccaat tctgccagaa gggaaattta tatgccctac
     7321 tatcgaccta ataattatga ttttgatgat atcgttgaaa gtctcaaagt tgttaacagg
     7381 tatggcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnntaataaa aataggaaag accgacggaa aaataggttt gaaaatgatc
     7501 caatggcgca atttcaatat tgaccccgac tggtacctgg gtaaaattgg tattacggat
     7561 accggtgaat ctatgggtgt taaacaacta caggaattga tcaaagaaga atttcccgat
     7621 ttgaaatatg gttatttcaa tcctccgata gagagaataa agnnnnnnnn nnnncaatgg
     7681 attttgctca ttaaatacca taaggtcttg tcttttcaaa atcttaacat ttaaaatata
     7741 gctgtttatg caagttgcat ggtgtttgaa tcgttactga caggtgtgaa caataatgat
     7801 ttgcgccacc aatcaccaaa atgtagattt ggatgaataa cgcctacgct atgtctgcat
     7861 cccttgaagc cagaaaccgt tcccaggctt atatgatcac agctggtttt accggcctga
     7921 tgctactgct gatgttctta ctcaaatggg atatgccctc atttgaaaga acagttcagg
     7981 aaccaggcat agaagtagaa ctaaacctgc ctgaagaacc agaaacactg gccgatggcg
     8041 gtggtggtgg cggtaatcca gtacaggcag ctggcgatcc cggtatagcg ccttatactc
     8101 ctccagcccc tggagaagaa caagattcaa aagacattga ggaagacaac gataaagatg
     8161 cacccgaagt aaccaaaccg gttgtagcaa agcccaaagc aacaaccata accaacacat
     8221 cagttgccaa aactgaacct aaacctgttg tagaaacccc tgccccaaga gttcccaaag
     8281 ctgttttggg caaaacgact actggaaccg gccgtggcgg tggagccgaa gattacgaca
     8341 gggctggtgg aagtggaacc ggtagcggtg ttggcaatgg atcaggcaca ggcggtggta
     8401 gtggttcagg taatgggggt ggcaatggct ccggtactgg cagcggcaat ggtcctaaag
     8461 taacacgggg agaccgaaaa attgttcgca gctattcatt tgaaggagat ctcgaccgag
     8521 ctaccatata tgctaatgtc aacgtttctc ccgagggagt gggcacattt gtcagcattg
     8581 ccaaaggttc ttcaaactct ggcagcgcct ataagaatgc cattatacaa tatctgcaac
     8641 gtattcgctt cgacaaatct gaccatgaat caatgatcac ggtacagttc aatttcaggg
     8701 taaactaatc tttcaataca atttcagcga agccacgata attatcgtgg cttcgctttt
     8761 tatatttttg tataacatga attaccagga aacaattgat tacttgttta cccgtctccc
     8821 tatgttcagc aggattggtg cagctgctta taaaaaagac ctgactaata ctgttgagtt
     8881 atgcactttt ttaggtaacc ctcaaaaaaa gttcaaatca atacatattg caggaaccaa
     8941 cggcaaaggc tctgtaagcc acatgctggc agctatattg caaacagcgg gatacaaaac
     9001 aggactttat acatccccac atttgaaaga tttcagggag agaatnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnntttt gagataactg tggcaatggc ttttgattat tttgctcatg
     9181 aagaagttga tattgccgtg attgaaacag ggctgggcgg aagactggac agcacaaatg
     9241 tcatcttccc ggtactttct gtcatcacca atattggatg ggatcacatg aatatgctcg
     9301 gggataccct tgaaaaaatt gcattcgaaa aagccggaat tatcaaagaa ggagttccgg
     9361 ttgttattgg tgaagtctta gcggaaacac tcccggtatt tgaaaatgca gctaaagaaa
     9421 a
//
LOCUS       DAORLS010000108         9384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3956_length_9384_cov_2.205595, whole genome shotgun sequence.
ACCESSION   DAORLS010000108 DAORLS010000000
VERSION     DAORLS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3956_length_9384_cov_2.205595"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK977_09475"
     CDS             complement(<1..434)
                     /locus_tag="PK977_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18389.1"
                     /translation="MEKTIKRRLRLAGIMIMAGVAAFSQAKSGLENYNYMSSGQAYSW
                     MPVAHYQAKKGFYAELRYNYEDVKTVSFYSGKTFYTGKERQISIIPMIGISTGTFTGI
                     SAACKAEAEEGLFFFSTEFQYSKDLKNTDGSFMFSWSEAGVSV"
     gene            complement(406..990)
                     /locus_tag="PK977_09480"
     CDS             complement(406..990)
                     /locus_tag="PK977_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18390.1"
                     /translation="MSGPSKEKEYNIYVRLDEQTVHSYFNQHDPAPLYKRQLGHEFEQ
                     YIYNSLLTSSRDAVFKYQFICLDYADKRFIAPVTQAIRSHFNLKIYLKQEEFRKFKIR
                     TFRLMVVSLIIVLSFQAVLSYIGGLMDERMSGALHNTLDVFSWVILWKPIDRLIFYWN
                     PFKKDLHLLERLANAEVIILEKTKIIGKDNKKAS"
     gene            complement(1173..1607)
                     /locus_tag="PK977_09485"
     CDS             complement(1173..1607)
                     /locus_tag="PK977_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF18391.1"
                     /translation="MNEIQILLVEDNEGDILLTLEAFKELKVNNSVVVVRDGAEAIEF
                     LKSEGQFAGTKLPHLILLDINMPRLNGIEVLDFIKKDEKLKRIPVVMLTTSSSESDIS
                     ECYEKSANCFITKPLDFGSFLGVVKSIESFWFNVAQLPRPAA"
     gene            complement(1647..>2045)
                     /locus_tag="PK977_09490"
     CDS             complement(1647..>2045)
                     /locus_tag="PK977_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF18392.1"
                     /translation="EDNEGDIILTTEAFKDMEMENRLTVVKNGEEALRYLKKQGQYEG
                     VPSPQLVLLDINLPGIDGKQLLGLIKKDEELKDIPVVMLTCSEDDSDISDCYDNQVNW
                     YITKPIDYDKYTKVMHEIEAFYVSFVAYNK"
     assembly_gap    2046..2088
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2100..>2411)
                     /locus_tag="PK977_09495"
     CDS             complement(2100..>2411)
                     /locus_tag="PK977_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF18393.1"
                     /translation="THLLDNAIKFRSADRPVIHIDAARQNHQWQISISDNGIGIDPAF
                     HERIFDIFKQLHPEEAGYKGTGTGLTVCKKIAELHDGRIDVFSEAGRGSMFVVSIPEK
                     I"
     assembly_gap    2412..2422
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2423..2880)
                     /locus_tag="PK977_09500"
     CDS             complement(<2423..2880)
                     /locus_tag="PK977_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF18394.1"
                     /translation="MSNRQFLHTGLVDTSTVLKDTGDELILLQEQLANLVKETNQFTY
                     IITHDLQAPLRMVTGFLELLERRYADKLDAGAKQYIEYSVKGANRMKSLIFDLLEYSR
                     LSSVKHEFDVVDMREVLEEVIGKLSVDISSSGADVIIGALPGVWADRKMMV"
     assembly_gap    3067..3076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3077..3413)
                     /locus_tag="PK977_09505"
     CDS             complement(<3077..3413)
                     /locus_tag="PK977_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF18395.1"
                     /translation="MSILIRDLLEFSRSGNKAAKKEPVNLESVIDLVQKDMSIVMQDT
                     SAGLYVPEQMPVVEGTHSALYRLFLNLVSNGIKFRKKDTSPQVSILLNEQNDCWEFTV
                     QDNGIGVPEK"
     assembly_gap    3450..3680
                     /estimated_length=231
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3681..4985)
                     /locus_tag="PK977_09510"
     CDS             complement(<3681..4985)
                     /locus_tag="PK977_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF18396.1"
                     /translation="MKRYKRKILVFLIVSLLIVVGSFTILFLNFRKQLSLQDRILVLQ
                     ANLTHTDSIITNLLQLESDKRAFQITSDISYLRNFYAIRTGCNGSIDRLKSNVSDKSI
                     ISIISEVDRLMELRLANMDSGLAVFRKNGPEAAILFMQDPSKRSIRERLAIQLHSLKS
                     SFQEALQSTTAGINKKSEQNIIGLLVLVLVFISLMLIATRTFRRAQHRVIMNHLKFKE
                     AQRIAKIGSWEWNFKTNRLKWSQEQFRIFGETRGEFELTYKGYLNHFPLQEQQKIIDL
                     MHNAIETKTEFIIEHDIYRRDGTSLTVLERGSLILDKDNNPIGIFGTTQDISERKDYE
                     KQLLQAELKYKAIIDNSADGIYQSTPDGKFVMVNPSMAGIFGYASPAEMINQVQNIGI
                     QIYADPEDRQNMAEIITRQGHIKNYEMQVLKKDGSRIWVSANI"
     gene            complement(4986..6149)
                     /locus_tag="PK977_09515"
     CDS             complement(4986..6149)
                     /locus_tag="PK977_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF18397.1"
                     /translation="MLQESIRRYAYSDTEGKVRKLINSLHVGILVFNSQEEVLFSNKF
                     AHDMLGLSEKQLRMKGPYHPEWSVVYEDGSPFSCEMSPVLVALKTQKPVTNLIVGINR
                     PLTGDRAWLLVNAEPILTDSGEVSEIVCSFSDITQRKAIEDKLTSLYQSLESRAFDLA
                     CSNADLERFVYVATHDLQEPLRLITSFLQLLKKKYDSQLDEQASEYINYAVDGSRRIK
                     KLILDLLEYSKFSIDRDGFKLLDPGKVIEDVKHSLCSLLKQHHAIIDVKPLPVIYADP
                     SLLHQLFKNLLTNSVKYRGESTPQISIDCITQGDDYLFSVSDNGIGIDPGYSEKIFDL
                     FQRLHKNEAFEGTGVGLAICEKIIRLHRGSIWVESMPGKGSTFYFTIPQKQQW"
     gene            complement(6568..8757)
                     /locus_tag="PK977_09520"
     CDS             complement(6568..8757)
                     /locus_tag="PK977_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF18398.1"
                     /translation="MKTQWFAVKQLISSLEAEKPYLSVISSTGKIFKANSRMKKEFAL
                     PDPSHAEVNFLDLVHPVNQDGFRKLLESTSKGISVSTELYLKNGVFHPMKWAINTIEI
                     KGQKAFLCGGRKLLSNDRLELFNQLGKENYQVLIESLNAAVIFHDINGELIASNQKTA
                     ELFSTTLERLYQLTDIRSLWDNMWHVTTETGEIVKFDETPFMRSLKSGKRESQLLVVS
                     LANGEKRWMHFTSQPLINKQTSIAYTAVSNVIDLTEEKRALLELVNRKAIFGAFMKQS
                     PNLAWVVDEDATLLEANNIFYSFFGLKESETKNKNILELVPDSVSKALYEKHLQVIES
                     GKASGFTEKVKWANGTEFAFFINIFLIEGLSGKKILGGEAINLTDKYESEKRLKEAND
                     RLLLLTRATTDAIWEWDMQTGKVFRNDALMDMIGYEADQHKGLSWWLRSIHPEDRNRV
                     SDKVKQSTDSGRQSWEDEYRFKCADGNYKHMRDRGYIVYENGLPVKMIGSLQDISELK
                     KMEDELFVEKLRQQKELSETIVRVQEKERTRIGHELHDNVNQLLSTTKLFVDMINPST
                     EDEKMFKSKSLEYILTAIEEIRKLSKELVVPQLKGNSLVGNIKHLIEDIELSGTLQIK
                     FTHDHENDILSPGKKVTIFRILQEQMKNILKYSKASQVDILLQCKDKFAELTIIDNGI
                     GFDPQKTRKGIGLSSIYERTRFYNGKMDINSSPGNGCKLVVGIPCYE"
     gene            complement(8787..>9090)
                     /locus_tag="PK977_09525"
     CDS             complement(8787..>9090)
                     /locus_tag="PK977_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF18399.1"
                     /translation="ETDSPRQIHSANNYDEARRLLAELNPDIVLLDINLPGKNGIEIL
                     KMIRSENKFSTVLMLTNHADAYYRQQCMELGADYFLDKSEDFGLVPGIINQLTLQH"
     assembly_gap    9091..9122
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2624 a         1955 c         1494 g         2984 t
ORIGIN      
        1 acacttactc ctgcttctga ccagctgaac ataaaacttc cgtcagtatt tttaagatcc
       61 ttgctgtatt gaaactctgt actgaaaaaa aataatcctt cttctgcctc agctttgcac
      121 gcagcggaaa ttccagtaaa agttccggtt gaaatcccaa tcattggtat aatacttatt
      181 tgcctttctt taccggtata aaatgtttta ccactataga atgaaactgt ttttacatct
      241 tcataattgt aacgtaattc ggcataaaat ccttttttag cctggtaatg agccaccggc
      301 atccacgaat aagcctgccc gctactcata taattataat tctccaatcc gcttttagcc
      361 tgagaaaaag cagcaacacc ggccataatc ataatccccg ccagcctaag acgccttttt
      421 attgtctttt ccaatgattt tggttttttc aaggattatt acttcagcgt ttgcaagtct
      481 ttccagtaaa tgaagatctt tcttaaaagg attccagtaa aatatcaacc tgtcaattgg
      541 tttccacaaa atgacccagc taaaaacatc caaagtattg tgcaaagcac cagacatgcg
      601 ttcatccatt aatccaccta tataagacag aactgcctgg aatgataata caataatcag
      661 actgacaacc atcagccgga atgttctgat cttaaatttt ctgaactcct cctgcttgag
      721 atatattttg agattgaaat gagatcgtat agcctgggtc accggtgcaa taaaacgctt
      781 atctgcataa tcaaggcaaa taaactgata tttaaacacc gcatcccggc ttgaggtgag
      841 aagtgagtta taaatgtact gttcgaactc atgtcccaac tggcgtttgt aaaggggagc
      901 agggtcatgc tgattaaaat aactatgtac cgtctgttca tctaaacgaa catagatatt
      961 atattctttt tcttttgacg gaccggacat aatagttttt tgtaaggatt ggattattca
     1021 tgccatagca tatattctat accaaggcat tattactcta caacgcaata gtattattat
     1081 taattctttt ttaagatagg aaaaatacca gtttagggat aaacataatt atatataaga
     1141 actactactt aaatttttgt aacctgtctg tgtcaggctg caggccgggg caactgtgca
     1201 acattaaacc agaatgattc aatacttttt acaacaccca ggaagcttcc aaaatcaaga
     1261 ggcttagtaa taaaacaatt ggctgatttt tcgtaacatt cactaatgtc agactctgat
     1321 gaagatgttg taagcatcac aactggtatt cgttttagct tctcatcctt tttaataaaa
     1381 tcaagtacct caatgccatt tagcctgggc atattaatat ccagcaagat caggtgcggc
     1441 agcttagtac cggcaaactg tccctcactc tttagaaact ctatagcctc tgctccgtct
     1501 ctcacaacta caacactatt gtttactttt aactctttaa atgcttcgag ggtcaataga
     1561 atatctcctt cattgtcttc aactaacaaa atttggattt cattcatagt aatggtttta
     1621 tagttagcag gggctgaata gtaattttat ttattatatg caacaaatga aacataaaag
     1681 gcctcaatct catgcatcac ttttgtgtat ttatcatagt ctataggctt ggtaatatac
     1741 caattcacct gattatcata acaatcacta atatcactgt catcctctga gcaggtaagc
     1801 attactacag gtatatcttt aagctcttcg tccttcttta tcagtccaag aagctgtttt
     1861 ccatctatac caggcaggtt aatatcaagt aataccagtt gcggagatgg cacaccttca
     1921 tactggccct gctttttcag gtatcgaagt gcttcttctc cattttttac aactgtaagc
     1981 cggttttcca tttccatatc tttgaaagcc tcagtggtaa gaataatgtc accctcatta
     2041 tcctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ttatagatat
     2101 tagatttttt ctggtattga aacaacaaac atactccctc tgccagcttc ggaaaacaca
     2161 tctattcgac catcatgcag ttctgcaatt ttcttacaga cggtaagtcc tgtcccggtt
     2221 cctttatatc ccgcttcctc gggatgcaac tgtttaaata tgtcaaatat tctttcgtga
     2281 aatgccggat ctattccaat tccgttgtcc gaaatactaa tttgccattg atgattctgc
     2341 cgggcagcat caatatgaat cacaggcctg tcagcacttc ggaatttgat tgcattgtcc
     2401 agaagatgag tnnnnnnnnn nnaccatcat ttttctgtca gcccatactc cgggaagtgc
     2461 cccaattatc acatcagcac cagaagaaga aatatcaaca gaaagcttac caattacttc
     2521 ttcaagcact tctctcatat ccactacgtc aaattcatgc ttcacagaac taagccttga
     2581 atactccagc aaatcaaaaa tgagactctt catccgattg gcccctttta cactatattc
     2641 aatatattgc tttgcaccag catctaattt atcagcatac ctcctttcca atagttcaag
     2701 gaaaccagtt accatacgta gcggggcctg caggtcatgt gtgattatat aagtaaactg
     2761 gtttgtttcc tttaccaggt tagccaactg ttcttgcaag aggattaact cgtctccagt
     2821 atcttttaaa accgtagacg tatcaactaa acctgtatgc aaaaattgcc tgttgctcat
     2881 atagattgtt taagctctca tttttggaag ggtaaaatga aatgccgttc ctttaccgaa
     2941 ttctgatgtc atccagattt taccaccgtg tgtttcgact atcttcttac aggtaactaa
     3001 tccaagacct gttccggggt aatcttcttt tctgtgaagt cgctgaaaag cctggaagag
     3061 tttgggnnnn nnnnnncctt ctcaggtaca cctattccat tatcctgcac cgtaaattcc
     3121 cagcaatcat tttgttcatt cagaagaatc gaaacctgtg gcgatgtatc ttttttcctg
     3181 aactttattc cattactaac caaattcaga aatagcctgt atagtgcact atgtgtacct
     3241 tctaccactg gcatttgttc cggaacatac aatcctgctg aagtatcctg catcacaata
     3301 ctcatatcct tttgaacaag atcaataact gactcaagat taacaggttc ctttttggcc
     3361 gctttatttc cagaccttga aaactccaaa agatcccgga tgagtatact cattctgtca
     3421 gcgccatcct ttgccctaaa tatgtacccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn aatattggca ctaacccaaa ttctgctacc gtcttttttt
     3721 aatacttgca tttcataatt cttaatatgc ccctgtcgtg taattatttc agccatattc
     3781 tgccggtctt caggatccgc atatatctgt atgcctatat tctgtacctg atttatcatc
     3841 tctgctggcg atgcatatcc aaaaataccg gccatagaag ggttcaccat tacaaatttt
     3901 ccatcaggag tactctggta gattccatct gctgaattat caataatcgc tttatatttt
     3961 agctctgcct gtagcaattg tttttcataa tccttccttt cagaaatatc ctgtgtagtc
     4021 ccgaatatgc caatagggtt attatcctta tctagaatga gactccctct ttccaataca
     4081 gttaaagaag taccgtccct tctgtatata tcatgttcaa taataaattc ggttttcgtc
     4141 tctattgcat tatgcataag atctataatc ttctgttgct cttgtaaagg gaaatgattg
     4201 agatacccct tgtaggttaa ttcaaactca cctcttgtct ctccaaaaat tctaaactgc
     4261 tcctggctcc acttaagcct gttggtttta aaattccatt cccagctacc gattttggca
     4321 atccgttgtg cttctttaaa cttcaggtgg ttcatgatca cccgatgttg agccctccta
     4381 aatgttctgg tggctattaa catcaaagaa ataaatacca gtaccagtac aagcagccca
     4441 attatatttt gttcactttt tttatttatt ccagcagtag tagattgtaa cgcttcctga
     4501 aatgaactct ttaaagagtg tagctgtatc gctaatcttt cccgaatact tcgcttacta
     4561 ggatcctgca taaatagtat tgcagcctct gggccatttt tacgaaatac tgctagccca
     4621 gaatccatat tcgccaatcg tagctccata aggcggtcaa cttcactgat tatactaatg
     4681 attgatttgt ctgatacgtt ggattttaag cggtcaatac ttccattaca tcctgtcctg
     4741 attgcataaa aatttctcaa atagcttata tctgatgtta tctggaatgc ccttttatca
     4801 gattcaagct gaagaagatt agttataatg gaatcagtat gagtaagatt agcctgtaaa
     4861 acaagtatcc tatcctgtaa actaagctgc tttctgaaat ttaaaaaaag gatagtaaag
     4921 ctacccacta cgataagcag tgaaacaata aggaatacta atatttttct tttatatctt
     4981 ttcatctacc attgttgttt ttggggaatg gtaaaataaa aagtgctgcc cttacccggc
     5041 atagactcaa cccaaattga gcctcggtgc agcctgatga ttttttcaca aattgccagg
     5101 ccaacacccg ttccttcaaa ggcctcgttt ttatgcaggc gttgaaacag gtcaaagatt
     5161 ttttctgaat agccggggtc gataccgatt ccattatcgc ttacagaaaa aagatagtca
     5221 tctccttgtg taatacaatc aatacttatt tgtggagttg attccccacg atattttacg
     5281 gaatttgtca gcaggttttt gaataactgg tgcagcaatg acggatcggc gtaaataaca
     5341 ggtaaaggct tcacatctat aatggcatga tgctgtttta ggagagaaca caggctatgc
     5401 ttcacatcct caattacttt acctggatca agcagtttaa atccatcccg gtcaatgctg
     5461 aactttgaat attcaagcaa atcaagaatg agttttttaa ttctccggga accatctacc
     5521 gcatagttta tgtattcgga agcctgctca tcaagctggc tgtcatattt cttttttaat
     5581 aattgaagga aacttgtgat cagacgtaaa ggctcctgga ggtcatgtgt tgcaacataa
     5641 acaaaccgct caagatctgc gttggagcag gcaagatcaa aagctcttga ttcaagactt
     5701 tgatataaag aagtaagctt atcctcaatc gcttttctct gtgttatatc actgaaactg
     5761 cagactattt cacttacttc gccggagtca gttaaaattg gttctgcatt cacaaggagc
     5821 catgccctgt ctccggttag tggcctgttg attccaacga tcaggttggt tacaggtttt
     5881 tgtgttttta gtgcaactaa aacagggctc atttcacagg aaaacggaga gccatcctca
     5941 taaacaacac tccattccgg gtgatacgga cctttcatcc ggagttgttt ctctgaaagg
     6001 ccgagcatat catgagcaaa cttattactg aacaaaactt cttcctgaga gttaaaaaca
     6061 agtataccta catgcagact gttgatcagc tttctgactt ttccttccgt atctgaatag
     6121 gcatatcttc taatactttc ctgcaacatt taattgatgt ttaataaaat gttgaaatac
     6181 attatcagtc aggacatatg acctccttaa ttgatcgtat gtatgaacat caatcaaact
     6241 gcaaaaggga gttgaaaaaa gcagcgctaa tttgccggta attgcttgaa tcataataca
     6301 acttatcggt gaatgaactc ccgatatatc tattccggca acaggcacaa gccaaggcaa
     6361 agagttggtc ttttccatgc ggttttaaga tattggataa actgaactac aggcaactca
     6421 gtaattttca aaatgattac aggttgccgt actattctta cagtatgaaa taacggagaa
     6481 atatttgcta acacataaca accattaatt tattttgaca attaggtgtt attgcttaaa
     6541 gcaaatttgg tatagccatt aaattattta ttcgtaacaa gggataccca ctacaagttt
     6601 acagccattt cccggcgagg aattaatatc cattttgccg ttataaaaac gggttctttc
     6661 ataaatgctc gataagccaa tcccttttct tgttttctga ggatcaaatc cgataccatt
     6721 atctattatt gtcagttcag caaacttatc cttgcactga agaaggatat ctacctgtga
     6781 tgctttactg tacttgagaa tgtttttcat ctgctcctgt agaatacgaa atattgtaac
     6841 ctttttaccc ggacttaata tatcattctc atggtcatgt gtaaacttta tctgaagtgt
     6901 acccgacaac tctatatcct ctataagatg tttgatgttt ccaacaagac tattaccctt
     6961 taattgtgga accacaagct ccttggataa ttttcttatt tcttcaatag cagtaagtat
     7021 atattctaaa ctttttgatt taaacatttt ttcatcttct gttgacgggt ttatcatgtc
     7081 aacaaacaac ttggtagttg ataacagctg gttcacatta tcatgcagct catgcccaat
     7141 gcgggttctc tccttttcct gtacccgtac gattgtttca gataattctt tttgctgtct
     7201 taatttttct acaaaaagct catcctccat ttttttgagt tcagaaatat cctgtaatga
     7261 gccaatcatt tttaccggta atccattttc ataaacaata taacccctgt ctctcatgtg
     7321 cttatagttt ccatccgcac acttgaaacg gtattcatct tcccaagact gtcttccgct
     7381 gtcagtactt tgctttacct tatcacttac ccggtttctg tcttccggat gtatactcct
     7441 caaccaccag gatagcccct tgtgctggtc tgcttcatac ccaatcatat ccatcaaagc
     7501 atcattacga aacactttgc ctgtttgcat atcccactcc catattgcat cagtagtagc
     7561 tcttgttagc aataaaagac ggtcgtttgc ctctttcaat cttttttcag actcgtactt
     7621 atccgtaaga ttaatagcct cgccaccgag tatttttttc cctgataagc cttcaataag
     7681 aaaaatatta ataaaaaaag caaactcggt tccatttgcc catttcactt tctcagtaaa
     7741 gccagatgct tttcccgatt caattacctg taaatgcttc tcatacagtg cctttgaaac
     7801 tgaatcaggc acaagctcca gaatattctt gttttttgtt tcactctctt taagaccaaa
     7861 aaatgaataa aatatattgt ttgcctctaa tagggtggca tcttcgtcta ctacccatgc
     7921 aagattgggc gattgcttca tgaaggcccc gaaaatagct tttcgattta ccagttcaag
     7981 taaagctctt ttttcctcag ttaaatctat gacatttgaa accgccgtat atgcaattga
     8041 tgtttgctta ttaattaatg gctgagaggt gaaatgcatc cagcgttttt cgccatttgc
     8101 aagactaacc accagtaact gtgattccct ttttccgctt tttaatgaac gcatgaaggg
     8161 ggtttcatca aatttcacaa tttctcctgt ttctgtagtg acatgccaca tgttgtccca
     8221 caggcttctg atatctgtta attgatataa tcgctcaagt gttgtagaaa aaagctcagc
     8281 agttttttga ttggaagcaa ttagctcacc attaatatca tgaaagatca ctgctgcatt
     8341 gagactttca ataagaactt gatagttttc ttttcccagt tgattgaaca attcaagtct
     8401 gtcatttgaa agtaacttcc ttcccccaca caaaaatgct ttctggcctt ttatctcaat
     8461 cgtattaatg gcccatttca ttggatggaa aaccccgttc ttaaggtaca actctgtaga
     8521 aacggatata cccttactag ttgattccag taacttcctg aacccatcct ggttaacagg
     8581 atggacaaga tccaggaaat taacttccgc atgagacgga tcaggcaaag caaactcctt
     8641 tttcattcgg gagttagctt taaaaatttt accagtactg ctaatgactg acaggtaggg
     8701 cttttcagcc tcaagagaac tgattagttg tttaaccgca aaccactggg ttttcatact
     8761 tcactcttta tttttactaa tctatattaa tgctgcaggg taagctggtt tataatacct
     8821 ggcaccaggc caaagtcttc tgacttatca agaaaataat cggcccctaa ctccatacat
     8881 tgctgacgat agtaagcatc tgcatgatta gtgagcatca gtactgttga aaatttgttt
     8941 tccgacctga tcatttttaa aatctcaatg ccatttttac caggaagatt tatatcgagc
     9001 aagactatat ccgggtttag ctctgccagt agtcttctgg cttcatcata attattggct
     9061 gaatgtatct ggcgcggaga gtcagtttct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnaacaaagt tcatattatc gtcaactatg agtatgacag tatctttttt catgaggata
     9181 gtagcgtaat tttccacaaa ataatgtgga aagcccatcc tgcttgtacg ggtattgagt
     9241 aaaagtatgt agacggatta ctacaaaaaa agaggaatct tgcttgagct aaatcaactt
     9301 gttctcaact gcatacctgg ccaactctgc attgcttttc atttgcatct tttccagtat
     9361 tctggacctg taggtactta ccgt
//
LOCUS       DAORLS010000109         9380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_3960_length_9380_cov_2.095228, whole genome shotgun sequence.
ACCESSION   DAORLS010000109 DAORLS010000000
VERSION     DAORLS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_9380_cov_2.095228"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PK977_09530"
     CDS             <1..1027
                     /locus_tag="PK977_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18400.1"
                     /translation="FFRAQFTEAENYLTKYIDSKLPETDIQDQFLYAQLCWAKKDFSC
                     AVSKAEIVYVAMGDVTKPKVFRLLADAYFQKGDFANAKKYSDLFFLKKNPDDYISGDH
                     KIRADILSKTGGTTDEIFVNYLQGSVLDTVLSSKIDFLKQGAEFLKARGDSISRVREG
                     DLRLEIVKLKADKAGQRDFFDAGFAYYQGKNYEKADSIFDVFTDRYPDEVYGPMMQYN
                     IHRALDSTMEKGMAVPWAEKYLTLLEKDTAKNKKTIIGVASYLASYHANIVKDREKAL
                     EYLRKMLVLDPTNEIIKNNISVLEKAPATKPPSPSKGGAAPAPAKAGSPKPSAKTITH
                     KEIPIKT"
     assembly_gap    1081..1179
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1192..1587
                     /locus_tag="PK977_09535"
     CDS             1192..1587
                     /locus_tag="PK977_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRF18401.1"
                     /translation="MIHLLQTGLNTTDAGSYLPVALQVLIAVGLVAFLMAVTHWLGPK
                     RKTSDKLQNFTSGIESHGDARQPMAIKYFLTAILFVLFDVEVIFFYPYAVNFKGLGWD
                     GFWAVLMFVGFFLCGFIYIVKRGALKWED"
     gene            1688..2272
                     /locus_tag="PK977_09540"
     CDS             1688..2272
                     /locus_tag="PK977_09540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HRF18402.1"
                     /translation="MSRPVSYNLIQKPLVADISMAEMPEGHQGEGFFATSLDKVIGLA
                     RKNSIWPLPFATSCCGIEFMATMGSHYDLARFGSERVGFSPRQCDLLMVMGTIAKKMG
                     PVVKQVYLQMAEPRWVLAVGACASSGGIFDTYSVLQGIDQVIPVDVYVPGCPPRPEAI
                     IDGVIRIQDLVGKENLRRRNTEQYKALLESYGIQ"
     gene            2275..2781
                     /locus_tag="PK977_09545"
     CDS             2275..2781
                     /locus_tag="PK977_09545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRF18403.1"
                     /translation="MALSNEHIKQRLVEKFGDQVSGFEEPYGMLTFEAPREMNLKVLN
                     FLYDDAELKFRFLTDITGVHYPDKTGQELAVVYHLHNLEDNVRIRFKVFANVNQPDVY
                     TATGLFSAANWMERETFDFFGINFVGHPNLKRILNVEEMDYFPLRKEFPLEDQTRIDK
                     DDEMFGRG"
     gene            2812..4035
                     /locus_tag="PK977_09550"
     CDS             2812..4035
                     /locus_tag="PK977_09550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRF18404.1"
                     /translation="MSEHHIKLPEGSIEKTTTTLNLGPTHPATHGVMQNILELDGERI
                     VSTEQTIGYIHRAFEKLAERRPLAQITTLTDRLNYCSSPLNNMGWHMTCEKLLGVKTP
                     KRVDYLRVIIMELARIADHLICNSIVGVDAGAYTGFLYVMQYRELIYEIYEEVCGARL
                     TTNIGRIGGFERNFNDIAFQKLEKFLKEYPAVLKEFENLFQRNRIFMERTMGTGAISA
                     ERALNYGFTGPNLRAAGVDFDLRVHSPYSSYEDFQFNIPVGTTGDNYDRWLVRNQEMW
                     QSLSIIEQAYQKVQEFKGAEAEVYHADVPEYYLPPKADVYTKMEALIWHFKIIMGETE
                     IPAGEVYHSIEAANGELGFYLISDGGRSPYRLHFRRPCFVYYQAFEELIKGSMLSDAI
                     MTMSSLNLIAGELDA"
     gene            4091..4594
                     /locus_tag="PK977_09555"
     CDS             4091..4594
                     /locus_tag="PK977_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRF18405.1"
                     /translation="MSLSFSDEKLKEVQRIVDRYPQGKQKSALIPVLHLAQEEFGGWL
                     STETMDYVASVLNLKPIEVYEVATFYSMFNLKPVGKYMFEVCQTGPCMLNGSDDIVKY
                     IHEKLGIKPGETTSDGMFTLKTVECLGACGYAPMMQQGKYYKEHLTKEKIDAIIAECR
                     STAAAKN"
     gene            4598..5014
                     /locus_tag="PK977_09560"
     CDS             4598..5014
                     /locus_tag="PK977_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF18406.1"
                     /translation="MKKLLVIVLLLAAVVAGAQTEETKLATTIKEFHQALVNRNTVSI
                     NQQTDKVLSYGHSNGWVETKNELIKNLETGYMKYNSYNEDSLQVVINGNVAYARFVAD
                     ITATLNGKEGNFRLKVLEVWVKKGKRWLLFARQAVK"
     gene            5060..>6122
                     /locus_tag="PK977_09565"
     CDS             5060..>6122
                     /locus_tag="PK977_09565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HRF18407.1"
                     /translation="MGRKLLLDKANIEGIRYYDVYRREGGYRSVEKALKSMTPDQVTD
                     EVKKSGLRGRGGAGFPTGMKWSFLAKPEGEPRYLVVNADESEPGTFKDRYLMEFIPHL
                     LIEGMITSSYALGSNRSYIYIRGEYAWIVDILEQAIAEAKNNGWLGKNILGTGFDLEI
                     YVQRGAGAYICGEETALLESLEGKRGNPRIKPPFPAVKGLWDCPTVVNNVETITAVVP
                     IVNEGGEEYAKIGVGKSTGTKLISACGNINKPGVYEIDMTISVEEFIYSDEYCGGISN
                     GRKLKACIPGGSSVPILPANLLLKTAKGETRYMNYESLADGGFATGSMMGSGGFIVMD
                     DSQCVVRHTYTLARFYRHES"
     assembly_gap    6123..6275
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6433..>6631
                     /locus_tag="PK977_09570"
     CDS             6433..>6631
                     /locus_tag="PK977_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF18408.1"
                     /translation="MAEEKKLFKVTIDNITIDVEPGTTILNAARQIGGDVTPPAMCYY
                     SKLQGSGGKCRTCLVEVAAGSA"
     assembly_gap    6632..6641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6642..7545
                     /locus_tag="PK977_09575"
     CDS             <6642..7545
                     /locus_tag="PK977_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HRF18409.1"
                     /translation="DPRPMPKLVASCRTTVMDGMVVKNITSERVLDARAGVVEFLLLN
                     HPLDCPICDQAGECHLQDLSYEHGKEGTRYEFKRRTFEKEDIGPYIQLHMTRCILCYR
                     CTYVADQITNNRVHGILDRGDHAEISTYISKAIDNDFSGNMIDVCPVGALTDKTFRFK
                     NRVWFTKPVDAHRDCEKCCGKVTLWQRGDEVLRVTARKDQWGEVQSYDSKPGWICNTC
                     RFDKKHVNDWVIEGPTKISRHSVISQGHYVGMQKPKETMPEVMGGRAPKLLMDIHEIS
                     EVNSVDISALPGPATSKTFEKKEQ"
     gene            7555..8613
                     /gene="nuoH"
                     /locus_tag="PK977_09580"
     CDS             7555..8613
                     /gene="nuoH"
                     /locus_tag="PK977_09580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRF18410.1"
                     /translation="MYLLALDLWLIVEKAALILCIIGLSFFIAMYTTLAERKVAAWMQ
                     DRRGPSRAGPWGIFQPLADGGKLFFKEEIIPLASSKFLFILGPAMAMTTALLTSAVIP
                     WGGNLHLFGRDISLQIADINIGILYIFGVVSLGVYGIMIGGWASNNKFSLLAAIRGAS
                     QMISYELAMGLALIAVLMLTGTLQMSGIVGNQMTGNNLWHIFLQPLGFIIFFVCALAE
                     CNRTPFDLPEAENELNFGYHQEYSSMKLGFYLFAEYINMFISSAVMATLFFGGYDILP
                     FVDETKWGLSENLLTILGFGALMAKTFFFLFVFIWIRWTIPRFRYDQLMGLGWKKLIP
                     LALANMIITAAVILWLKK"
     gene            8664..9209
                     /gene="nuoI"
                     /locus_tag="PK977_09585"
     CDS             8664..9209
                     /gene="nuoI"
                     /locus_tag="PK977_09585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRF18411.1"
                     /translation="MQLTKRATAVDRRPMNALEKIYLWNILKGMAVTFKHMFKKRPTI
                     NYPEQKRPFSQVFRGLHVLNRDAEGRENCTACGLCAVACPAEAITMEAAERLPGEEHL
                     YREEKYAAKYEINMLRCIFCGLCEEACPKDAIYLSQTFAPANYGRKGFIYKKEDLLIP
                     HPINEPEAYKKALGEKAALKN"
BASE COUNT         2725 a         1668 c         2252 g         2473 t
ORIGIN      
        1 ttttttcagg gcacagttta ctgaagcgga aaattacctg actaagtata tagacagtaa
       61 attacctgaa actgatatcc aggatcagtt cctgtatgca cagctttgct gggctaaaaa
      121 agattttagc tgcgcagtca gtaaagcgga aatagtatat gtggcgatgg gggatgtaac
      181 caagcccaaa gtgttccgcc tgctggcaga tgcctatttc cagaaaggtg actttgctaa
      241 tgccaaaaaa tacagcgacc tgtttttcct gaaaaagaac ccggatgact atatctcagg
      301 tgatcataag ataagagcag acattttatc gaaaacaggt ggtactactg atgagatatt
      361 tgttaactac ctgcagggtt ctgttttaga tacagtatta agctcaaaga tcgattttct
      421 aaaacaaggg gcagagtttt tgaaggcaag gggcgatagt attagcaggg taagggaagg
      481 cgatctcaga cttgaaattg taaagttaaa agctgacaaa gcaggtcaaa gagatttctt
      541 tgatgccggg tttgcttact atcagggtaa gaattatgaa aaagcagaca gcatctttga
      601 tgtgttcact gacagatacc cggatgaagt ctacggtccg atgatgcagt ataatattca
      661 cagggctctt gacagtacca tggaaaaagg catggcagta ccctgggctg aaaagtacct
      721 gactcttctt gaaaaagata ctgccaaaaa caaaaaaaca ataatcggtg tggccagtta
      781 cctggcgtct tatcatgcca atattgtaaa agaccgtgaa aaggcattgg aataccttag
      841 aaagatgctg gtgcttgatc ccacaaatga gataataaaa aataatatta gtgtgctgga
      901 aaaagccccg gctacaaaac caccttcccc ttctaaaggc ggagcagcac cggctccggc
      961 caaggcaggt agtccaaagc catctgctaa gacaataaca cataaagaaa ttcctataaa
     1021 gacttagaac tcggttttct tcttttacaa aagagaatcc cttgctgtaa tggcggggga
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt caaaagagct tatgattcac
     1201 ctccttcaga ccggtttgaa cacaacagat gccggatcct atcttccggt agcgctacaa
     1261 gtgcttatag cagtgggttt agtagcgttt ttgatggcag taacacattg gctgggaccc
     1321 aagcgtaaaa cttcagacaa gttgcagaac tttaccagtg gaatcgagtc acatggtgat
     1381 gcccgccagc ccatggcaat caaatacttt ctaacggcta tcctttttgt gttatttgat
     1441 gtggaagtga tctttttcta tccctatgca gtgaatttca aagggctcgg gtgggatgga
     1501 ttttgggctg tattgatgtt tgtaggattt ttcctatgtg gattcatata tatagtaaaa
     1561 agaggagcat tgaagtggga agactgagta attgaatatt tgtttatgga atattgaaga
     1621 tgggggtgaa ccccgggtgt taagagttgt tttacatgtg aatcttcaat attaaaatgg
     1681 taataacatg tctcgtccgg tatcatacaa ccttatacaa aagccgctgg tagctgatat
     1741 cagtatggct gaaatgcctg agggccacca gggtgagggt ttttttgcca cctcgctgga
     1801 taaagtaatc gggctggcaa ggaaaaactc tatatggcct ttaccttttg ccacatcctg
     1861 ctgcggcatt gagttcatgg ctactatggg ctctcattac gaccttgccc gttttggctc
     1921 cgaaagggtt ggcttttctc cccgtcagtg tgatctttta atggttatgg gcactattgc
     1981 taagaaaatg ggtccggtgg taaaacaggt ttatctgcaa atggcagaac cccgttgggt
     2041 actggctgta ggtgcctgtg ccagcagtgg gggtattttc gatacctata gtgttttaca
     2101 gggaattgac caggtgatac cggtggatgt ttatgtgccc ggttgtcccc caagacctga
     2161 agccattatt gatggtgtta taagaataca agatcttgtt ggtaaggaaa acctcagaag
     2221 aagaaatact gaacaataca aagcattact agaaagctac ggaatacaat aataatggcg
     2281 ttatcgaacg aacatattaa acagaggctg gttgagaaat tcggggatca ggtttctggg
     2341 tttgaagagc cctatggcat gctgactttt gaagcaccca gggaaatgaa cctgaaagta
     2401 ctcaacttct tatatgacga tgccgaattg aaatttcgct tccttacaga tattactgga
     2461 gtgcattacc cggataaaac cgggcaagaa ctggcagttg tatatcacct gcacaacctg
     2521 gaagataatg tgcgaatccg gtttaaagtt tttgccaatg tcaatcagcc tgatgtttac
     2581 acagcgacag gtctgttctc tgcagctaac tggatggaaa gggaaacttt tgacttcttc
     2641 gggataaact tcgtggggca tccgaatctt aagaggatat tgaatgtgga ggaaatggac
     2701 tatttcccgc tgagaaaaga atttcccctg gaagatcaga ctagaattga taaggatgat
     2761 gaaatgtttg gaagaggttg attagtttat gtgtcaattt gaaaattaat aatgtcagaa
     2821 catcatatca aattacccga aggctcaatt gagaaaacaa ctaccacgct gaacctgggt
     2881 cctacccacc cggctaccca tggtgtgatg cagaatatcc tggaactgga tggggaaagg
     2941 atcgtttcta cggaacaaac catcggttac atacaccggg catttgaaaa gctggctgaa
     3001 cgccgtccgc tggcacaaat cactacatta accgacaggt taaactactg ttcttctccg
     3061 ctgaataata tgggctggca catgacctgt gagaaattgc tgggagtgaa aacgccaaag
     3121 agggttgact atctcagggt cattattatg gaacttgcca ggattgccga tcacctgatc
     3181 tgcaattcta tagtaggggt ggatgcgggt gcttataccg gcttcctgta tgtgatgcag
     3241 taccgtgaac tgatttatga aatatacgaa gaggtttgcg gagccagact tactaccaat
     3301 attggccgca tcggcggttt tgaaagaaac tttaatgata tcgcttttca gaaattggag
     3361 aaattcctga aagaatatcc ggcagttctg aaagagtttg aaaacctgtt tcagcgcaac
     3421 aggattttca tggaaagaac catgggaacc ggtgcgatca gtgcagaaag ggcattgaat
     3481 tatgggttta ccggaccaaa cctccgtgca gcaggtgttg actttgattt gcgggttcat
     3541 tcgccatatt ccagctacga agattttcaa ttcaatatac cggtaggtac taccggcgat
     3601 aattatgacc gctggctggt gcgtaaccag gaaatgtggc agagcctgag cattattgaa
     3661 caggcttacc aaaaagtaca ggagtttaaa ggagcagaag cagaggtata tcatgctgat
     3721 gtgcctgagt attacctgcc tccgaaagct gatgtatata cgaaaatgga agcgttgatc
     3781 tggcatttca aaatcatcat gggagaaaca gaaattccgg caggagaggt gtatcactcg
     3841 atagaagcgg ctaacggaga gttaggattt tacctgatca gtgatggcgg acgttcaccg
     3901 tatcgtttgc atttccgcag accttgtttt gtgtattacc aggcttttga agagttgata
     3961 aagggttcga tgttgagcga tgcaattatg acgatgagta gcttgaacct gattgcagga
     4021 gaattggatg cataaaaaat ttatatgtca tgctgagcct gccgaagcat gactaaaatg
     4081 aataaagaaa atgagtttga gtttttcaga cgaaaaatta aaagaagtac aacgcattgt
     4141 tgaccgctat ccgcaaggaa agcagaagag tgctttgata cctgttttgc acctggcaca
     4201 ggaggaattt ggtggctggc tcagtacaga aaccatggat tatgtggcat cagtattaaa
     4261 cctaaagcca atcgaagtat atgaagtggc taccttctat agcatgttca accttaaacc
     4321 agttggtaaa tacatgtttg aagtttgtca aacaggtccc tgtatgctaa atggcagcga
     4381 tgatatcgtg aaatacattc acgaaaagct gggaatcaaa ccaggtgaaa caacgtctga
     4441 cggcatgttt acccttaaaa cggtagaatg tctcggtgcc tgtggttatg cacccatgat
     4501 gcagcaggga aagtattata aagagcatct gaccaaagaa aagatagatg ctatcattgc
     4561 tgaatgcagg agcactgcag cagctaaaaa ctaatacttg aaaaagttat tagtcatagt
     4621 tcttttatta gcggcagttg tggccggtgc acaaaccgaa gagacaaaac tggcaacaac
     4681 gataaaagag tttcaccagg cattggttaa tagaaatacg gtttccatta accagcaaac
     4741 ggataaagta ctgagttacg gacatagcaa tggctgggtg gaaactaaaa atgagctgat
     4801 caaaaacctg gaaacaggtt atatgaaata taacagctat aatgaagaca gcctgcaggt
     4861 tgtgatcaat ggaaatgtgg cgtatgcaag gtttgtggca gatattaccg ctacattgaa
     4921 tggtaaagaa ggaaacttcc gtttgaaagt actggaagtg tgggtaaaga aaggaaaaag
     4981 atggttgttg tttgcgaggc aggcagtaaa ataaaaaaag cttatagcta ttagctattg
     5041 gctaatagct tttctaaata tgggaagaaa attattatta gacaaagcaa acattgaagg
     5101 catcaggtat tatgatgtat accgccgtga aggaggctat cgcagtgtgg aaaaggcgtt
     5161 gaagtcaatg acgccggacc aggtgacgga tgaagtaaag aaaagtggcc ttcgtggtcg
     5221 tggcggcgca ggatttccaa caggaatgaa gtggagcttt ttggcaaaac cggaaggaga
     5281 accccgctac ctggtggtaa atgcggatga atctgaaccg ggaactttca aagaccggta
     5341 cctgatggaa ttcattcctc atttactgat tgagggaatg atcacatctt cttatgcatt
     5401 aggatctaac cgttcctata tctatatccg cggagagtac gcttggatcg ttgatatttt
     5461 agaacaagcc atcgctgaag caaaaaacaa tggctggtta ggtaaaaata ttttaggtac
     5521 cggctttgac ctggagatat atgttcagcg gggagcaggt gcttacatct gtggcgagga
     5581 aacagccttg ctggaatcat tggaaggaaa aagaggaaat ccacggatta aaccaccatt
     5641 ccctgcagta aaaggattgt gggattgccc tactgtggtc aacaatgtgg agacgatcac
     5701 tgcagttgtt cccattgtga acgaaggcgg agaagaatat gccaaaattg gtgtaggtaa
     5761 atccactggt acaaaactga tttctgcctg cggcaatatt aataagcccg gtgtgtacga
     5821 gatcgacatg accatttcag tagaagagtt tatttacagt gatgaatatt gcggcggaat
     5881 ttccaacgga agaaaactca aagcttgtat tccaggtggt tcttcagtac ctattttgcc
     5941 agctaatctt ttgcttaaaa cagcaaaggg tgaaacaagg tacatgaatt acgagagcct
     6001 tgccgacggt ggattcgcta ccggttcgat gatgggttca ggtggtttca tcgtgatgga
     6061 tgacagtcag tgtgtggttc ggcataccta tacgttggcc agattttatc gtcacgaaag
     6121 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgatg cagcagcatg gccggtggcc
     6301 gcagcgatcc gtcatttccg tgatgaattt gaatggcatg ttaccaaccc ggatgatgca
     6361 cagcgaagaa attatggact ggcacattat gctgatccga taaaggtggc aacacctgca
     6421 taaatgaatt gtatggctga agagaaaaaa ttatttaaag taaccattga caacatcact
     6481 atagatgttg aacccggtac taccatcctt aatgcagccc ggcaaattgg tggtgatgtc
     6541 acacctcctg ccatgtgcta ttacagtaag ctgcagggaa gtggtggtaa gtgcagaacc
     6601 tgtttagtag aagtggcagc tggttctgct gnnnnnnnnn ncgatccgag accaatgcca
     6661 aaactggtgg cgagttgccg cactacagta atggatggca tggtggtgaa aaatattacc
     6721 agtgaaagag tgctggatgc gagagccggt gttgtagaat ttttgttatt gaaccatccc
     6781 ctggattgtc ctatttgcga ccaggctggt gaatgtcacc tgcaggatct cagttatgaa
     6841 catggaaagg aaggcacccg ttacgagttc aagagaagaa catttgaaaa agaagatatt
     6901 ggtccataca tacagttaca catgacaagg tgcattcttt gttatcgttg tacatatgta
     6961 gccgaccaga ttaccaacaa ccgggttcat ggtatattag acaggggcga tcatgccgag
     7021 atatcaactt atatctctaa agcaattgat aatgatttca gcggaaatat gatcgatgtt
     7081 tgtcctgtgg gagcattaac ggataaaaca ttccgcttta aaaacagggt atggtttacc
     7141 aagccggtgg atgcacaccg tgattgcgaa aagtgctgtg gtaaagtaac cttatggcag
     7201 cgtggagatg aagtattgcg ggtaacagcc agaaaagacc agtggggtga ggtgcagagt
     7261 tatgacagta agccgggctg gatttgcaat acctgccgtt ttgataagaa gcatgtaaat
     7321 gattgggtga ttgaggggcc aactaagatc agccgtcact ctgtaatatc acaagggcat
     7381 tatgttggca tgcaaaaacc taaagaaaca atgccggaag tgatgggcgg aagggcgccg
     7441 aagctgctga tggatattca tgaaatcagt gaagtgaaca gtgttgacat cagtgctttg
     7501 cccggaccgg caacaagtaa aacatttgaa aagaaagaac aataatagca gaccatgtat
     7561 ttactggcat tagacctgtg gttgatcgta gagaaagcag cattgatact atgtatcatt
     7621 ggcctctcct tttttattgc tatgtacacc actttggctg agcgtaaagt ggctgcatgg
     7681 atgcaggatc gccgtggtcc cagccgtgca ggtccatggg gaatttttca gccgcttgcg
     7741 gatggcggca agttgttctt caaagaagaa attatcccgc tggcgtcttc aaaattcctt
     7801 ttcatattgg gtcctgccat ggccatgaca actgccctgt tgacaagtgc agtaattcca
     7861 tggggaggaa accttcattt gtttggccgt gatatttccc tgcagatagc agatattaat
     7921 attggtatcc tttacatttt tggtgtggtt agtttgggtg tttacggtat aatgatcggg
     7981 ggatgggcat ctaataacaa attttcttta ttggcagcta ttcgtggtgc atcacaaatg
     8041 atctcatacg aattggctat gggtcttgca ctgatcgctg tacttatgct aacgggaacc
     8101 ttgcaaatga gcggcattgt tggaaaccag atgacaggca ataatctctg gcatattttt
     8161 ttgcagccac ttggttttat catctttttt gtctgtgcgt tagcagaatg taatcgtaca
     8221 ccatttgatc tgcctgaagc agaaaacgaa ctgaactttg gttaccacca ggaatattca
     8281 tccatgaaac tgggctttta cctctttgca gaatatatta atatgtttat cagcagtgcg
     8341 gtaatggcaa ccttattctt tggtgggtat gatattttgc cttttgtgga tgaaacgaaa
     8401 tggggattat ctgaaaacct gctgacaata cttggttttg gtgctttgat ggctaaaacg
     8461 tttttcttcc tctttgtctt catctggatt cgctggacga taccccgttt ccggtatgac
     8521 cagctgatgg gattgggatg gaagaaactg ataccattgg cgcttgccaa tatgatcatt
     8581 acagcagcag tgatattgtg gttgaaaaag taagtgaaga cataaatata tatttgcgaa
     8641 aattttaaaa gctgaaaagc tttatgcaat taacaaaaag agcaacagca gtagacagga
     8701 ggccaatgaa tgcattggaa aagatttacc tgtggaatat tctgaaggga atggctgtta
     8761 cttttaagca catgtttaaa aaaaggccta ctattaatta cccggagcag aaacgtcctt
     8821 tttcacaggt attccgtggc ctgcatgttt tgaaccgtga tgcggaagga agagaaaatt
     8881 gtacagcctg tggtttatgt gctgtagcct gcccggctga agctattacc atggaagctg
     8941 cagaaagatt acctggtgaa gagcatttgt acagggaaga aaaatatgca gcaaaatatg
     9001 aaataaatat gctgcgctgt attttttgtg gtttatgtga ggaggcctgc ccgaaggatg
     9061 ctatctatct ttcccagaca ttcgcaccgg ccaattatgg aagaaaagga tttatatata
     9121 aaaaggaaga cctgctgatc cctcatccaa tcaatgagcc ggaggcttat aaaaaggcgt
     9181 tgggtgaaaa agcagctttg aaaaattaat tgaaaaaact aattgctgtc cgctttggcg
     9241 gttgagatat gaacattacg caaatactat tctggtttct ttctgttatg gccatcttca
     9301 gtggcctgat gatgattacc agtaaaaatc cagttcacag tgtgctgtgg ctgatagtca
     9361 ctttttttgc tatttccggt
//
LOCUS       DAORLS010000110         9260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4019_length_9260_cov_2.021293, whole genome shotgun sequence.
ACCESSION   DAORLS010000110 DAORLS010000000
VERSION     DAORLS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_9260_cov_2.021293"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..698)
                     /locus_tag="PK977_09590"
                     /pseudo
     CDS             complement(1..698)
                     /locus_tag="PK977_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(695..1525)
                     /locus_tag="PK977_09595"
     CDS             complement(695..1525)
                     /locus_tag="PK977_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRF18412.1"
                     /translation="MNTRVTIQGYEGSFHQVAAQMYHGKNVQVIPCATFRDVVKMGSN
                     KKESDGAVMAIENSIAGSILPNYNLLQKSNLRITGEIYLQIRQNLLVNRGVRLEEIKE
                     VHSHTMALQQCYEFLDKYKWKLVETDDTALSAKHIHQHKSKHIAAIASKLAADLFNLD
                     VIASNIHTMKNNYTRFLMLQREDMALPVNEADKASVTFHTDHTRGSLAKVLTKIAEGG
                     INLSKLQSFPIPGSNFKYAFHADMEFGTISQFEDVIQEIRPLTESIKIYGTYKRGLWK
                     "
     gene            complement(1728..>2085)
                     /locus_tag="PK977_09600"
     CDS             complement(1728..>2085)
                     /locus_tag="PK977_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRF18413.1"
                     /translation="ADLNMREERRGDRRGEKIEGRQARDTKGRSFTGGANYQRLFVNL
                     GTKDGFYKASFLQFILDMSDLRKDVLGRIDMKEMNSWVEVDASAAKKMIKAIDGKNYR
                     GRKIRMNDAESGGRRK"
     assembly_gap    2086..2102
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2103..3388)
                     /locus_tag="PK977_09605"
     CDS             complement(<2103..3388)
                     /locus_tag="PK977_09605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF18414.1"
                     /translation="MNTFESLGIEQGLLNSIHALGFTQPTPIQEKAIPVLLQGTKDFI
                     GLAQTGTGKTAAFGLPLLQLINKEDRYPQALVICPTRELCLRITNDMDKFKNKADRIA
                     VTAVYGGASIGDQIRSLKKGTHIIVATPGRLIDLIERKAVNLEQVSYIILDEADEMLN
                     MGFKDDIEFVLKNTPNRESTWLFSATMPPEIRQVSKRYMEQPFEITIGKVNSGNVNID
                     HQYYLTQHVNRYETLKRIIDFNPGLYGIVFTRTKADAQDITESLIREGYDIEALHGDL
                     TQAQRDKVMARFREKSIQLLIATDVAARGIDVQGITHVINYELPDDTEVYTHRSGRTG
                     RAGRSGIAVSIVTPKEVYRLRQIEKLVKTQFHKMDIPSGKDVCRKQFFHFIDKMLQAD
                     ISHGEYETYLPVLKEKFAHVEKEEILQKVAALEFDR"
     gene            complement(3584..3775)
                     /locus_tag="PK977_09610"
     CDS             complement(3584..3775)
                     /locus_tag="PK977_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18415.1"
                     /translation="MKKFFAIAFIAATMVACNNSGEKKEETKDTTAAPAPAPVQDTTV
                     TPAPAQDTTAAPAADTTKK"
     gene            3957..4538
                     /locus_tag="PK977_09615"
     CDS             3957..4538
                     /locus_tag="PK977_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF18416.1"
                     /translation="MKSLIDEKELLTGLAASDKKAVETIYQENFNMVQALIINNNGSA
                     DDAKDIFQEAMVVLYEKVRSGSFELNCQIRTFVYSVSRRLWLKRLQQQNRYSDPGDSM
                     ESVVAVEEDLEAHEQRNAEFEMMEKAINNLGEPCKSLLEAYYLQKQNMQVIASRFGYT
                     NADNAKNQKYKCLVRLKKIFFTHYKNGNSDRGH"
     gene            4522..5628
                     /locus_tag="PK977_09620"
     CDS             4522..5628
                     /locus_tag="PK977_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRF18417.1"
                     /translation="MIEDIKILDTVERYIRGEMKPDERLQFENLRKTNTEIDLLVVEH
                     TLFLQQLNHFGERKTFRSSLNDVHIDLTEQGKIDSMKLKGKAKVVYIWKRYRRVAAIA
                     AVFAGVTTLTITALVNAVSPKADNAKITELSRDIQDIKRVQDNQGKEVNNLRKKITTV
                     PAPAFKAGGSGFLIDGKGYLVTNAHIIRNSKNIVVINNKGEQFKAIVAKVFTDKDIAI
                     LKIDDEAYKSVGSLPYGIRKSSTDVAEPIYTLGYPRNEIVYSEGYLSAKTGFNGDTLS
                     CQLGIAANRGNSGGPVFNQDGEVIGVISTKEMEAEGVAFAIQSKFIYNAIDELKKDTS
                     YQSLKLPAKSSVRGLNKKDQVKKIQDYVFMVKGD"
     gene            complement(5708..>6541)
                     /locus_tag="PK977_09625"
     CDS             complement(5708..>6541)
                     /locus_tag="PK977_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF18418.1"
                     /translation="VEGFGPSGETIMDYSIFDAIRAGFGKVVFIIRKEYAENFKAIFE
                     PKLRGRIQTEYVFQELSFFTEGYSFPKERAKPWGTAHAVLCAKDAVNEPFAVINADDF
                     YGHNAFNNAAAFLNVGCNPENYAIVGYDLLKTLSEHGTVNRGVCSLDHLGNLASVTER
                     INIAKKGNVIVCDDGLEPAELPLNTRVSMNFWCFDPSFFNYTQQLFTEFLKENGQELK
                     SEFFIPIVADRFIREGGKIEIIPTSSSWFGVTYKEDAPYVKQSLDELIKNGEYPTSLW
                     S"
     assembly_gap    6542..6719
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6720..7566
                     /locus_tag="PK977_09630"
     CDS             <6720..7566
                     /locus_tag="PK977_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRF18419.1"
                     /translation="SVPAYKEKNLEVDILRIDKIHPVVSGNKWFKLRYYLEEAQQQKK
                     STIVTFGGAWSNHIVATAAACRLNNLSSIGIIRGEESETPSSTLLEARQLGMQLVFIS
                     RDAYKSKELPASLRNRDDCYIISEGGYGIKGAKGAATILDHAIEKNYTHICCAAGTGT
                     MTAGLLVHPSQADTISVSVLKNNFQLEDDIRHLVNNKKKPVIIHDYHFGGYAKHTPAL
                     LSFMNEFYKQTGIPSDFVYTGKLFFTINDLVSNNYFKSGSKLLAIHSGGLQGNRSLSK
                     GTLIF"
     gene            7635..8429
                     /locus_tag="PK977_09635"
     CDS             7635..8429
                     /locus_tag="PK977_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18420.1"
                     /translation="MKKVGLVYLAFTLLSGLLAAQDTLPNFTVKNMGNNRIIIGWVNN
                     LPDVKQISIQRSFDSLTGYKTILTVPDPAAVQNGYADMKAINDHMFYRLYIQQDKGMY
                     RFSKAKRPFKDTSKVAEPVAKTDPTIKTENISSSDSVRIMDSVTKAKPFVVKIDGFPG
                     TDSIASPNPVTVKNKPTAFVPSLYVYTNRDGYVRVSLPDEEKPKKYSIKFFDEDNKLL
                     FELKDIKQKNFKLDKTNFYHAGWFRFELYEDGKLLEKHKFYLEKDF"
     gene            complement(8412..9137)
                     /gene="lipB"
                     /locus_tag="PK977_09640"
     CDS             complement(8412..9137)
                     /gene="lipB"
                     /locus_tag="PK977_09640"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRF18421.1"
                     /translation="MKQQEVIFRDLGRMDYQSAWDYQEQLLKENVRLKTEAREKGIDP
                     SLVSTSNYFLFVEHLPVYTLGKSGNKENVLLDDEGLKERNIQFFTTNRGGDITFHGPD
                     QIVGYPIIDLEKFYTDIGRYLRNLEEVIILTLANYGLKGERSPGETGVWIEPEVPGKE
                     RKICAMGVRCSRWITMHGFALNVNTDLSYFNHIIPCGIVNKQVTSLEKELGKKITVAE
                     VKERIKENFEKVFETKLVSEVLL"
BASE COUNT         2643 a         2023 c         1744 g         2655 t
ORIGIN      
        1 taatgagttt aactcaatta caaaatcctt tgctccttta acagtcagca aactcttcgg
       61 gctatcattg agtataaaac tgtatggatt atcatgtaca agcagtatct tatttttcct
      121 cgcaaacctt accagttttt caaataattt cacggtaggc aaatgcccgg tcggcatttg
      181 cggatagttc acaaacatca gcttgacctt cttcagtttt ctttcttcca gtttatcaaa
      241 gtcaggctgg taataatttt tcgccgtcag tttatattca acacatttac cccctgccag
      301 tttcacagca ctggtatagg taggataccc gggattagga accagtcatc acccctatca
      361 agatatgcca tgcagatatg tagaatacct tctttgctgc caatcagcgg taatatttct
      421 gtatcagggt tcagctcaac atcataccat tttttatacc agtcactcat cgcttttctc
      481 agaacaggcg aacctttata agactggtaa gcatgtacag tgggcttagc agcttcctcc
      541 tgcaacgttt ttataacatc cgggtgcggc ggtaaatcag ggcttccaat gccgagatta
      601 ataatattct ttccggcttt attcagctct tcaatctccc gcaacttatg cgagaagtaa
      661 tattcaccga ttccttttag tcgtttactt gttctcattt ccataatccc cttttataag
      721 taccataaat tttaattgac tccgttaagg gccggatttc ctgaatgacg tcttcaaatt
      781 gactgattgt gccaaattcc atatcggcat gaaaagcata tttaaaattg gatccgggaa
      841 tcggaaaact ctgcagcttg ctcaggttga tacctccttc ggcaatcttt gtcagcactt
      901 ttgccaggct tccccgggtg tggtcggtat gaaatgtaac ggacgcttta tccgcttcat
      961 taactggtaa agccatatcc tctctttgca gcatcaggaa cctggtgtaa ttatttttca
     1021 tcgtatgaat gttagatgct attacatcca ggttgaataa atcagcagcc agtttactgg
     1081 caatcgcagc aatatgttta cttttatgct gatgaatatg tttagcactc aacgcagtat
     1141 catcagtttc taccagtttc catttatact tatctaaaaa ttcataacac tgctgcaatg
     1201 ccatagtgtg tgaatgcact tctttaattt cttccagcct tacgccccta tttaccagga
     1261 ggttttgccg gatctgtaag tagatctcac ccgttatcct gagattactt ttctgtaaaa
     1321 gattataatt aggtaaaatg ctgcctgcaa tcgaattttc tatggccatt acggccccgt
     1381 cactttcttt cttgttagaa cccattttaa cgacatcccg gaatgtggca caagggatca
     1441 cttgtacatt tttcccatga tacatctgag cagctacctg gtggaagctg ccctcgtagc
     1501 cctgtattgt aactcttgta ttcatatact aaaacaaaaa tcccggcctg ttctggccgg
     1561 gactctttat gttgtgtttt ttagttgttc actttatttt caacaaaagc attcccggcc
     1621 tctttgcaaa aaagaagtaa taaaaataaa agctccagta tgcttttgtg atggtcatga
     1681 aacaaatgta gtttttgatc agtgtacttc caacaggaat tgaaaaatta ttttcgcctg
     1741 ccccccgatt cggcatcatt catccggatc tttctgcccc tgtaattctt accatctatt
     1801 gctttaatca tttttttggc agcagaggca tctacctcca cccagctatt catctccttc
     1861 atgtcaatcc gtcccaatac atcttttcgc aaatcactca tatcaagaat gaattgcaaa
     1921 aagcttgctt tgtaaaaacc gtcctttgtg cccaggttga cgaacaagcg ttggtaatta
     1981 gctccgcctg taaaacttct tccctttgta tccctggcct gtcttccttc aattttttcc
     2041 ccgcggcgat cgcctcttct ttcttcccgc atattaaggt ctgcannnnn nnnnnnnnnn
     2101 nnaagcggtc gaactccaat gccgccactt tctgcaatat ctcttctttc tctacatgag
     2161 cgaacttctc tttcaataca ggcaggtatg tttcatattc accgtggctg atatcggcct
     2221 gcagcatctt gtcaataaaa tggaaaaact gtttgcggca aacatctttg ccactgggta
     2281 tatccatttt atgaaactgt gtttttacta atttttcaat ctggcggagc ctgtacactt
     2341 ctttgggtgt aacaatagaa acagcaatac cgctgcggcc tgcccttccg gttctgccgc
     2401 tgcgatgggt gtacacttcg gtatcatctg gcaattcata attgataaca tgtgtaatac
     2461 cctgcacatc aatccccctt gcagctacat ccgttgcaat taacaattgt atactctttt
     2521 cacggaagcg ggccattact ttgtcccgct gagcctgtgt aagatcaccg tgcaacgctt
     2581 caatatcgta tccttccctg atcagcgatt ctgttatatc ctgggcatcg gctttagtac
     2641 gggtaaaaac aattccatat aagccgggat tgaagtcaat gatgcgtttc agcgtttcat
     2701 aacggttcac atgctgggta agataatact ggtggtcaat attgacattc ccggaattca
     2761 cttttccaat agtgatttca aaaggctgtt ccatgtacct tttgcttact tgccggattt
     2821 ccggaggcat tgtagcgctg aaaagccagg tactctcccg gttaggggta tttttcagaa
     2881 caaactcaat atcatcctta aaacccatat tcagcatttc atcggcctca tctaatatga
     2941 tatagcttac ctgctccagg ttaactgcct ttctttctat cagatcaatc aaacggcccg
     3001 gtgtagcaac aataatatgg gtgccttttt tcaggctcct tatctgatca ccaatggaag
     3061 caccgccata aactgccgta acagcaatac ggtcggcttt atttttaaac ttatccatat
     3121 cattcgttat ccgcaagcaa agttcccggg tgggacaaat aaccaatgcc tgcggatagc
     3181 ggtcttcttt attgataagc tgaagaagcg gcagaccaaa ggcagccgtt tttccagtac
     3241 ctgtttgtgc aagtccaata aaatcttttg ttccctgcaa caaaacaggg attgcttttt
     3301 cctggatagg tgtgggctgg gtaaaaccca atgcgtggat agagttcaat aaaccctgct
     3361 ctatgcccag ggactcaaat gtgttcactt aataaatttt aaattgataa aaaccggctt
     3421 tagtttaacc ggttggccca gctgagcctt tcagatggag attaaccggg ccgcaaaggt
     3481 aacctttacc gggcgaaaag gttaatgtat tttgaatcat ctgtttaaaa tgaagaaggc
     3541 ccttccggtt ggaaaggcct tcgacgattg cttgccaatg aaattacttc ttagtagtat
     3601 cagcagcagg agcagctgta gtatcctgtg caggagcagg tgttactgta gtgtcctgaa
     3661 caggagcagg tgccggagca gctgtagtgt ctttagtttc ttctttcttt tcgccagaat
     3721 tgttacaagc taccattgta gcagcgatga atgcaattgc aaaaaacttc ttcattttgt
     3781 tgtgcttttt taaaggggtt tgttgaaata tttcgttgtt aatacccggt atgaaaaaag
     3841 gtaacccggc tttttaaatt ttttttacgt catgtcaggc accctgccct tattatttaa
     3901 tattttgcag tcaaacaggg ttacaatttg cagaatcctg tattaaataa cggattgtga
     3961 aatcactgat agacgaaaaa gagcttctga caggattagc cgccagcgac aaaaaagccg
     4021 ttgagaccat ttaccaggag aactttaata tggttcaagc cctgataatc aacaacaacg
     4081 gctcggcaga cgatgccaag gatatttttc aggaagcaat ggtagttctt tatgaaaagg
     4141 ttagatcagg gtcttttgag ctgaattgcc agatcagaac atttgtatat tctgtatcca
     4201 ggagattgtg gctcaaaaga ttacagcagc aaaaccgcta ttccgacccg ggtgacagta
     4261 tggagtcggt agtggcagta gaagaagatc tggaagccca tgagcagaga aacgccgaat
     4321 ttgaaatgat ggaaaaagcc atcaacaacc tgggcgaacc ttgtaaaagc ctgctggaag
     4381 cttattacct gcaaaagcaa aatatgcagg tcattgcctc ccgcttcggt tataccaatg
     4441 ctgataatgc caaaaaccag aaatataaat gcctggtaag gttaaaaaag atatttttta
     4501 ctcactataa aaacgggaac agtgatagag gacattaaga tattagatac cgttgaacga
     4561 tatatccggg gtgaaatgaa accggatgaa aggctccagt ttgaaaatct tcgtaaaaca
     4621 aatactgaga ttgacctgct ggtggtagaa catacccttt tcctgcagca gcttaaccat
     4681 tttggagaac gcaaaacctt ccgcagttca ttgaacgatg tgcatattga tcttacggaa
     4741 cagggcaaga ttgattcaat gaaactgaag ggtaaagcta aagttgttta tatctggaaa
     4801 agatacagaa gagtagccgc tatagccgct gtgtttgccg gcgtcactac ccttactatt
     4861 accgcactgg tcaatgcagt gtctcccaaa gcagataatg ccaaaatcac tgaattgagc
     4921 cgcgacatac aggatattaa aagagtccag gacaaccagg ggaaagaagt aaataatctt
     4981 agaaaaaaaa ttacaacagt acctgcacct gcctttaaag ccggcggctc aggattcctg
     5041 attgatggaa aaggatacct ggtcaccaat gcgcatatta tccgcaattc aaaaaatatt
     5101 gtggtcatca ataacaaggg agagcaattc aaagcaatag ttgccaaagt atttactgat
     5161 aaagacattg ctatccttaa aattgatgac gaggcttaca aatctgtagg atcactgccc
     5221 tatggcatcc gcaaatcatc cactgatgta gcagagccga tttataccct gggttatccc
     5281 agaaatgaaa tcgtttacag cgagggttac ctgagtgcca agacaggttt caatggtgac
     5341 accctcagtt gccagctggg tattgctgcc aaccgtggta acagcggtgg accggttttc
     5401 aatcaggatg gtgaagtgat aggcgttatc agtaccaaag aaatggaagc tgaaggagtg
     5461 gcttttgcca ttcaatctaa gttcatttat aacgctatcg acgaacttaa aaaagacacc
     5521 agctaccaat cattgaaact accagcgaaa tcatctgttc gtggcctgaa taaaaaagac
     5581 caggttaaaa aaatacagga ttatgtgttc atggtaaaag gagattaatc agcgcattcc
     5641 ctataaaagc aaaagcgatc aaccgtcagg atgatcgctt tttttatgaa acgatgtcaa
     5701 tggcagttca gctccaaagg cttgttggat actcaccatt ctttattaat tcatccagac
     5761 tctgcttcac atagggtgca tcctctttat aagtaacacc aaaccaggat gaacttgttg
     5821 gtatgatttc aatttttccg ccttccctga taaacctgtc tgctactatg ggaataaaaa
     5881 attcagattt taattcctgg ccattctcct tcaaaaattc agtgaaaagt tgctgcgtat
     5941 aattaaagaa agacggatca aaacaccaga agttcataga cacccttgta ttcagtggaa
     6001 gctcggctgg ttctaaacca tcatcacaaa caataacatt gccttttttg gcaatattga
     6061 ttcgttctgt aaccgatgcc agattcccta aatgatcaag gctgcacact cccctgttta
     6121 ccgttccatg ctctgacaag gtttttaaaa gatcataacc gactatggca taattctccg
     6181 gattgcaacc cacattcaaa aaagcagcag cattattaaa agcattatgc ccataaaaat
     6241 catcggcatt aatcacagca aaaggttcat taacggcatc ttttgcacac aaaaccgcat
     6301 gtgccgtacc ccatggcttg gctctttctt taggaaaact gtatccttct gtaaaaaagg
     6361 ataattcctg gaatacatat tctgtttgaa tgcggcctct cagcttaggt tcaaatattg
     6421 ccttgaaatt ttcagcatac tctttccgga taatgaaaac tacttttcca aagcccgcac
     6481 gaatagcatc aaaaatggaa taatccatga tggtttctcc cgaaggccca aaaccttcta
     6541 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     6721 tcagtacctg catacaagga aaagaatctg gaagtggata tactacgcat tgacaaaata
     6781 catcctgttg tttccggcaa caaatggttc aaacttcgtt attacctgga ggaagcccaa
     6841 caacaaaaaa aatcaacgat cgtaaccttt gggggagcat ggtccaacca tattgtagct
     6901 acagcagcag cctgccggtt aaacaatctc agcagcattg gcatcatacg gggtgaggaa
     6961 tctgaaaccc cctcatcaac attgctggaa gcccggcagt tgggaatgca attggttttc
     7021 atcagcaggg acgcctacaa atcaaaagaa ttaccagcat cattaagaaa cagggatgat
     7081 tgttacatca ttagcgaagg tggctatgga ataaaaggtg caaaaggggc cgctactata
     7141 cttgatcatg ccatagaaaa aaactacact cacatttgct gcgctgcagg aacaggaaca
     7201 atgaccgcgg gtctgctggt tcatccttct caagcggata caatcagcgt cagtgtacta
     7261 aagaataact ttcagctgga ggatgatatt cgccacctcg ttaacaataa aaagaaaccg
     7321 gttatcattc acgactacca ttttggcggg tatgccaaac atacccctgc actattatca
     7381 tttatgaatg aattttataa acaaaccggc atcccatctg attttgtgta cactggtaaa
     7441 cttttcttca caatcaatga ccttgtcagc aataactatt ttaaatcagg aagcaaattg
     7501 cttgctattc acagtggcgg attacaaggc aaccggtcac tcagtaaagg aacgttaata
     7561 ttttagtttt tcattccggc catcctatct ttgtttggtt caactaaatc ctgtcccgta
     7621 ttggcaaaac agatatgaag aaagtcggcc tggtatatct tgctttcacc ttgctatccg
     7681 gtttgcttgc agcacaggat acactcccca attttacagt aaagaacatg ggcaataacc
     7741 gtattatcat cggctgggtg aataatttgc ccgatgtaaa acaaatcagc attcagcgtt
     7801 ctttcgacag ccttaccggt tataaaacca tcctcacagt tcccgatcct gccgccgttc
     7861 aaaatggcta tgccgatatg aaagccatta atgatcacat gttttacagg ttatacattc
     7921 agcaggataa aggcatgtac cggtttagca aagcaaagag accttttaag gatacttcaa
     7981 aagtagcaga acctgttgca aaaacagatc ccacaataaa aacagaaaat atctcatcgt
     8041 cagattctgt gaggattatg gactctgtga caaaagcaaa gccctttgta gtaaaaatcg
     8101 atggctttcc gggtacagac tccattgctt cccccaaccc ggtaactgta aaaaacaaac
     8161 ctacagcatt tgtaccctct ttatatgttt ataccaaccg ggatggttat gtaagggtga
     8221 gtttgccgga tgaagaaaaa cccaaaaaat actccatcaa gttttttgac gaagacaata
     8281 aattgctttt tgaattaaaa gacatcaagc aaaaaaactt taaactggac aagactaatt
     8341 tttaccatgc cggctggttc aggttcgaat tgtatgagga cggtaagtta cttgagaaac
     8401 acaaattcta tttagagaag gacttctgac accagttttg tctcaaatac tttttcaaaa
     8461 ttttctttga tcctttcttt tacttctgca acagttattt tctttcccaa ctctttttcc
     8521 agggaggtca cctgcttgtt gacaataccg caggggatga tatgattgaa gtaagaaagg
     8581 tctgtattca cattcagggc aaaaccatgc atggtaatcc agcgactgca acgaactccc
     8641 atcgcacaaa tttttctctc cttaccgggc acttccggct caatccaaac cccggtttca
     8701 ccgggtgaac gttctccttt caggccgtag tttgcaagtg ttaaaatgat cacttcttcc
     8761 aggttgcgga gataacggcc aatatcggta taaaattttt ccaggtcaat gatcgggtaa
     8821 cctacaatct ggtcggggcc atgaaaagta atatcccctc ccctgttggt agtaaagaac
     8881 tggatattcc gctccttcaa accttcatca tccagcaata cattttcttt attaccgctt
     8941 ttgccaagtg tataaacggg cagatgctcc acgaacagga aatagttact ggttgaaacc
     9001 aatgacgggt caattccttt ttctcttgct tctgttttta gccgcacatt ttccttcagt
     9061 agttgctcct ggtagtccca tgccgactgg tagtccatcc tgccaagatc cctgaatatt
     9121 acttcctgtt gcttcatgat aaaatacaaa tttaaactaa agtggcttaa atgtttctta
     9181 tggctagtcc tccaaaaacc caggtatatt cactttagca ttcctgacaa aaaaccatcc
     9241 tttacctttg ccggctatgc
//
LOCUS       DAORLS010000111         9125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4109_length_9125_cov_2.627122, whole genome shotgun sequence.
ACCESSION   DAORLS010000111 DAORLS010000000
VERSION     DAORLS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_9125_cov_2.627122"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PK977_09645"
     CDS             complement(<1..1069)
                     /locus_tag="PK977_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18422.1"
                     /translation="MSNDQSFQAAAWRRLKKNKGALAGLVVIIIAFVIAVFAYFIAPD
                     PSPYANRIILEIGGEKPGFRQTFIKVKKDKDVAEASFFQQLISGKEDVYYYVPISRHE
                     KKGDSIVVEKFIDIGLSERQAYSISQTAPDPVVTKKFLLGTDKYGRDILSRIIVGTRV
                     SLSVGFITVLISLSIGLMLGSLAGYFRGRTDDIIMWFINVIWSIPTLLLVFAITLALG
                     KGFWQVFIAVGLTMWVNVARLVRGQVLSVRELEYIEATRALGFSHTRTIVRHIWPNIM
                     GPVMVIAASNFASAIVIEAGLSFLGVGVQPPQPSWGLMIKENYNFIITQNPMLALAPG
                     FAIMILVLAFNLLGNGLRDALN"
     gene            complement(1083..2198)
                     /locus_tag="PK977_09650"
     CDS             complement(1083..2198)
                     /locus_tag="PK977_09650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF18423.1"
                     /translation="MAILSFVILLFVCYSILIIYYWQSWKAIPEFKASVPATDITISV
                     IIPARNEKENIGLLLNALQKQNYPEYLTEIIVVDDHSIDGTASVVEQFSFAKLVQLKE
                     DSINSYKKKAIETGIAAATNELIVTTDADCIPGAGWLSVLAAFKKEKNSVFIAAPVVL
                     ANNNSILQIFQTLDFLVLQGITAASVHKNIHSMCNGANLAYERNSFYRVNGFSGIDTI
                     ASGDDMLLMHKIANQYPGQIHYLKAEDAIVTTSPMKTWREFLNQRIRWASKAKYYDDK
                     RITAVLILVYLFNLSFLVLWVAGFWCYHYWLYFTGLWIAKTIIEWPFVSSVAAFYNKK
                     SLMKYFFFFQPLHIFYTIFSGLLGQFGRYEWKGRKVK"
     gene            2265..2837
                     /locus_tag="PK977_09655"
     CDS             2265..2837
                     /locus_tag="PK977_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18424.1"
                     /translation="MSKILKRIILSFICPLICQVSFSQTNSGSDGILFANLVMGKHLS
                     SINDSLLCAEGSNSLYSPVSETSCKSFMVKKINGERFQIGNMIFSYTFVFTNIYDSSG
                     IIDHVSFMNTYSKAIMFNYQESFSSDYKEILKYFVNLFGKKGKTGEVYRNKNYYHHNL
                     VWQDENYKYILTRDIQKNFLGISCSVQAKK"
     gene            complement(2838..>3512)
                     /locus_tag="PK977_09660"
     CDS             complement(2838..>3512)
                     /locus_tag="PK977_09660"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF18425.1"
                     /translation="YAGTQFSFYAIDLPFHGKTDWKEGLNFTIQDLEAISLSVAGTGS
                     LPLTVMGFSLGGRMALSLYEVLPQKINKLILLAPDGLKVNFWYWLSTQTWMGNKFFAF
                     TMKHPGWFFGLLQLMNKLKLVNASIFKFVKHYIDDKQVRQLLYQRWTTLRKIKPGISN
                     IKAHIKQNNTKVRLVYGIHDRIILSSVGEKFRRGMEDYCDVNIIHSGHQVLHEKHARE
                     IIESLL"
     assembly_gap    3513..3573
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3737..4177
                     /locus_tag="PK977_09665"
     CDS             3737..4177
                     /locus_tag="PK977_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRF18426.1"
                     /translation="MPNNQFKKGELYSFITGKASTAIARRLQKKFNAAGLHITIEQWS
                     VLYHLWKQEGISQQELCNATFRDKPSITRLVDNLEKLNLVKRVASDNDRRINLIYLTR
                     QAQKLQEEAMTLAEETLNEALETVPPDKVDVCKEVLQIVYDNLK"
     gene            4212..6005
                     /locus_tag="PK977_09670"
     CDS             4212..6005
                     /locus_tag="PK977_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF18427.1"
                     /translation="MSTAAAAKTVLKGGEWLIRESSPEDTFTPENFSEEQVMIRDMCN
                     QFLDAEIFPILDPIDNLEPGLMKSLLTKAGDQGMLSASFPEEYGGLGKDFVTATIVNE
                     YLGAGHSFSVAIAAHTGIGTLPILYFGTPEQKQKYIPKLITGEWAGAYGLTEPNSGSD
                     ALSAKTTAKLSDDGKHYILNGQKCWITNGGFADVYTVFAKVDGKDFTGFIVERGTEGF
                     TQGPEEHKMGIKGSSTVQLYFQDCKVPVENLLGEIGKGHKIAFNILNIGRLKLAAAAI
                     GGSKRSIGNSIEYAKTREQFKQPIANFGAIKHKLAQMAIRVFACESALYRTAQWIGDK
                     EHELLAAGKPFGDALLGAAEEYAIECAMLKVDGSEVLDYVVDEGVQIHGGNGFSAEYN
                     ASRAYRDSRINRIYEGTNEINRLLTLDMTLKRAMQGRLNLMGPAMEVSKELMSIPDFG
                     ATDEAPFASERKLIANLKKAILLTAGAAVQKMMMKIENEQEVLMNIADMAIETFNAES
                     ALLRVMKMVDQNGEAASQYHLDMMRTYLYDAADKVNKSGKDAVNSFAEGDEQRMILMG
                     LKRFTKAEPFNTKEARRRVADKLIAEGRYCF"
     gene            complement(6002..6754)
                     /locus_tag="PK977_09675"
     CDS             complement(6002..6754)
                     /locus_tag="PK977_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18428.1"
                     /translation="MTWNSVMGFVSSFALFLPIALILALRLYNYKSFPALVVYYSSVF
                     IYNLMTEGYIPMSKQIVYNWGLINNLLDAPLMMLFLTYFSPSFLFTKRLKIAVAAFIL
                     FELVVIALRGLNVESITIILGPGIIMIAALCLHFFVRHAKMAVEHGKSTGKALIAGSL
                     LFAYGCFGLIYLMYYVFKTPHVADTFLVYFMAATVSALMMSAGIIYERKLVKKIKEVQ
                     ITRRELSEIYKDTKMAAPVRTAMLDFDKESWN"
     gene            6945..8123
                     /locus_tag="PK977_09680"
     CDS             6945..8123
                     /locus_tag="PK977_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF18429.1"
                     /translation="MIRLPFFLGLLIAVQGLSQTTGNNFLTGRTNGKLPYLEYGLGSD
                     RLGGAKMTFLDTGVVVKVVDSTVINYRVQLSGNHFAYLPKANFKRDSSVSIKPYYLTG
                     SWLVNGDDKYDYVSIALDEKLPYRSMQLINPSKLVVDIFGVTSNTNWITQRTSVKEVK
                     NVYHEQIEDDVFRMIIELNHSQHWGYSIYYQGNRLFIKIKRQPDELQVSKMKIAVDAG
                     HGGDNSGAKGITSGILEKHYTLMIAKEVEKMLTQAGAAVFMTRKEDVSVGMTERIEML
                     KNEDPDFLISIHLNSSVRDSVKGVSTYYRYIGFRPLTQYIQKAMLEMGMADFGNIGSF
                     NFSLNGPTDYPNCLLEVAFLSNREDEALILDPEFHKAVATQVLCGIQEWLRACEKDKP
                     "
     gene            complement(8120..8491)
                     /locus_tag="PK977_09685"
     CDS             complement(8120..8491)
                     /locus_tag="PK977_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF18430.1"
                     /translation="MLVLTAFVISGVAAVKAPQQKERNLKVLPIDISDEKLDSIMQTY
                     NKALGVKCEFCHVKKKVYPGDMDYASDAEPKKEKAREMMRMTIDINQKYFPYQPGERP
                     EYLNTITCKTCHRGETFPPED"
     gene            complement(8562..>9125)
                     /locus_tag="PK977_09690"
     CDS             complement(8562..>9125)
                     /locus_tag="PK977_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF18431.1"
                     /translation="YGINFLRGNINDWGAGPTKDVLTALDKTIAEGWADTSKLLVTGG
                     SYAGYLVAWIIAHDQRFAAACSQRGVYDLATFFGEGNAWRLVPNYFGGYPWDASAKAT
                     LERESPINYVQNIKTPYIIFHGDNDRRTGFVQSEMLYKSLKVLGRPVEYVRHPNATHE
                     LTRSGNNRQRIDQMLRTWEFFERWLRN"
BASE COUNT         2783 a         1784 c         1950 g         2547 t
ORIGIN      
        1 catttagggc atctcttaat ccatttccaa gcaaattaaa agcaagtacc aaaatcatta
       61 tcgcaaaccc cggcgctaaa gcaagcatgg gattttgagt gatgatgaaa ttgtaatttt
      121 ctttgatcat taatccccag ctgggttgcg gtggttgtac acctactcct aaaaaactca
      181 aaccggcttc aataacaatg gcagaagcaa aattgctggc ggcaatcacc attaccggtc
      241 ccatgatatt gggccagata tgtcttacaa tagtcctggt atgagaaaac ccaagggctc
      301 gggtggcttc aatgtattcc agttctctta ctgacagcac ctgccctctc accagcctgg
      361 ccacatttac ccacatggta agacctacag caataaagac ctgccagaag cctttgccca
      421 atgccagtgt aatggcgaaa accaatagta acgttggaat actccagata acattgatga
      481 accacatgat gatatcatct gttctgccac gaaaatatcc tgccagcgat ccgagcataa
      541 ggccaatgct cagcgagatg agtaccgtaa taaaaccaac gctcaggctt acccttgttc
      601 ctacaatgat tcggctaaga atatctcttc catatttatc ggtgcccagt aaaaatttct
      661 ttgttaccac cgggtcgggt gcagtttgtg aaatcgaata tgcctgtcgt tcagaaagac
      721 caatgtcaat gaatttttca acaacgatac tgtcaccttt tttttcatgc cgggaaatgg
      781 gaacataata gtatacatcc tctttgccgg aaattaattg ctgaaaaaaa cttgcttcag
      841 caacatcctt gtcttttttt actttaataa aagtctgtcg gaacccgggt ttttcaccgc
      901 caatctccag gatgatgcgg ttggcataag gagagggatc cggtgcaatg aaataagcaa
      961 agacagcaat aacaaaggca ataatgataa cgaccagtcc ggctaaggcg cccttattct
     1021 tttttagcct ccgccatgca gcggcctgga atgactggtc attgctcatg cggggaagat
     1081 acttatttta cttttcttcc tttccattca tacctgccaa actgtcccag caaaccggag
     1141 aaaatagtat aaaaaatatg cagtggctga aagaagaaga aatatttcat cagcgatttt
     1201 ttattataaa aggcagcaac ggatgaaacg aaaggccatt caatgattgt tttagctatc
     1261 cataacccgg taaagtaaag ccagtaatga taacaccaaa aaccggctac ccataaaacg
     1321 agaaaagaaa gattgaacag gtataccaat ataagaacgg ctgttattct tttgtcatcg
     1381 taatatttag ccttgctggc ccagcggata cgctggttaa gaaattctct ccatgtcttc
     1441 atgggagaag tggttacaat agcatcttct gctttcaggt aatgaatttg ccccggatat
     1501 tggttggcga ttttatgcat cagcagcata tcatcgccgg aagcgatagt gtcaatacca
     1561 gaaaacccat ttactctata aaaagagttt ctttcgtagg caaggttcgc tccgttgcac
     1621 atagagtgga tgtttttatg aacggaagct gctgtaattc cctggagcac taagaagtcc
     1681 agggtctgga aaatttgtag tatcgagttg ttgttggcta atacaacagg ggctgcaata
     1741 aaaacagagt ttttttcttt tttaaatgcc gccagcactg atagccaccc ggcaccggga
     1801 atacaatctg catctgtggt gacaatcagc tcatttgttg ccgcagcaat tcctgtttca
     1861 atcgctttct ttttatatga attaatactg tcttctttca gttgtacaag cttagcaaaa
     1921 gagaattgct caacaacact tgccgttcca tctatagaat ggtcatctac tactattatt
     1981 tcagttaagt attctgggta attttgtttt tgtaaagcgt ttagcaacag acctatattt
     2041 tctttttcat ttctggccgg aataataact gagatggtaa tatcagtagc aggaacactt
     2101 gctttaaatt ctgggatggc tttccagctt tgccagtaat aaataatgag aatgctgtag
     2161 caaacaaaaa gaagtatgac gaatgaaagg atagccatgc aggataaaaa acaaatctaa
     2221 ctgaaaagtt tttatataag atttgtaaaa actaacttgt ctgtatgagc aaaattttga
     2281 agagaataat tcttagtttc atttgcccgt taatttgcca ggtgagtttt tcccaaacaa
     2341 attccgggtc tgatgggatt ttgtttgcaa accttgtaat gggcaaacat ttatcttcta
     2401 ttaatgactc actattgtgt gccgaaggca gtaattcgct ttattcaccg gtatcagaaa
     2461 ctagttgcaa aagttttatg gttaaaaaaa taaatggaga gagatttcaa atcggcaaca
     2521 tgattttctc ttatactttt gtttttacca atatctatga ctcttccgga ataattgacc
     2581 atgtatcgtt catgaatact tatagtaaag cgattatgtt taattaccag gaaagttttt
     2641 caagtgacta taaagaaatt ctgaagtatt ttgttaatct atttgggaaa aagggtaaaa
     2701 ctggagaagt ttataggaat aaaaactatt accatcacaa tttagtatgg caggacgaaa
     2761 actataaata cattcttacc agggatattc aaaagaactt tcttggtatt tcctgttcag
     2821 tacaggcaaa aaaataatca caataaactt tctataatct cccgggcatg tttttcatgc
     2881 aatacctggt ggccggaatg aataatgttg acatcgcagt agtcctccat ccctcttctg
     2941 aacttttcgc caacagaaga aagtataata cggtcgtgaa tgccataaac cagccgcact
     3001 ttagtgttgt tctgttttat atgtgcttta atattgctta ttcctggttt tatcttccgc
     3061 aaggttgtcc atcgctggta aagcagttgc cgcacctgtt tatcatcaat atagtgcttc
     3121 acaaatttga agatgcttgc attaacaagt ttcagcttat tcattagttg caataaacca
     3181 aagaaccagc ccgggtgctt catagtaaaa gcaaagaatt tgtttcccat ccatgtttgt
     3241 gtagaaagcc agtaccagaa attgactttt aagccatcag gcgccagcag gattagttta
     3301 ttaatttttt gtggaaggac ttcatacaac gaaagtgcca ttctaccacc caaactgaaa
     3361 cccatcactg tcaggggtaa actgccagta cctgctacgg acaaactgat ggcttcaaga
     3421 tcctgtatag taaaattcag cccttctttc caatccgttt ttccgtgaaa gggcaggtca
     3481 atggcgtaaa aactaaattg agtaccagcg tannnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccatgaa aacagataac cggcctttct
     3601 ccgctaccat accggtagta tctgatacag gaatttttgt atgtgaggct tctctcttcc
     3661 agggcaatta tttagaaaat taaaattgga gtaatcaaat atagtgttat ttttggatag
     3721 ttgtataaac aactatatgc caaacaacca atttaagaaa ggagagctgt acagctttat
     3781 caccggtaaa gcttctactg ccatagcaag aaggctgcaa aagaaattca atgcagccgg
     3841 tctgcatatt actattgaac aatggagtgt attgtatcat ctctggaaac aagaaggtat
     3901 tagtcaacag gagttgtgca atgctacttt tcgggataag ccgagtataa cccgcctggt
     3961 ggataacctg gaaaaactga acctggtaaa aagggtggct tcagataatg accggaggat
     4021 aaacctgata tacctgacca ggcaggcgca aaaattgcag gaggaagcga tgacactggc
     4081 agaagaaaca ttgaatgagg cgctggaaac agttcctccg gataaagtgg atgtatgtaa
     4141 agaagtatta caaatcgttt acgataacct gaaataacta acgatatact ttaaactcaa
     4201 aatttttaac catgagtacc gcagcagcag caaaaacagt attaaaaggt ggcgaatggc
     4261 tgatcaggga atcctcacct gaggatacat tcactcccga aaatttttca gaggagcaag
     4321 tgatgatccg ggatatgtgt aaccagttcc tggatgcaga gatattccca atactggatc
     4381 caattgataa cctggagccc ggtttaatga aaagcctgtt aacaaaagca ggagaccagg
     4441 gaatgttgtc tgcttctttt cctgaagagt atggcggact ggggaaggat tttgtaacgg
     4501 ccactattgt gaatgaatac ctgggtgcag gccactcttt ttctgtggcc attgctgccc
     4561 ataccggcat cggcacacta ccgattcttt attttggaac tcctgagcaa aaacagaaat
     4621 acattcccaa acttattacc ggcgagtggg caggtgccta tggtcttacc gaaccaaata
     4681 gcggaagtga tgccttaagt gccaagacaa cggctaaact aagtgatgat ggtaaacatt
     4741 atatcctgaa cggacagaaa tgctggatta ccaatggtgg ttttgctgat gtgtacaccg
     4801 tgtttgccaa agtagacgga aaagatttta ccggctttat tgtagaaaga ggaacagaag
     4861 gatttaccca gggacccgaa gaacataaaa tgggtatcaa aggttcttcc actgttcagt
     4921 tgtatttcca ggactgtaaa gtaccggtag aaaacctgct gggtgaaatt ggtaaaggac
     4981 ataagatcgc tttcaacatt ctgaatattg gccgtttgaa actagcagct gctgccattg
     5041 gtggttccaa acgttcgatt ggcaattcca tcgagtatgc aaaaacaaga gaacagttca
     5101 aacaaccgat tgccaatttc ggcgctatta aacataagct ggcgcaaatg gccatccggg
     5161 tctttgcctg cgaaagtgct ttatatagaa cagcgcaatg gatcggggat aaggaacatg
     5221 aattattggc cgcaggaaag ccattcgggg acgcattgct gggtgctgct gaagaatatg
     5281 ccattgaatg tgccatgctg aaagtagacg gaagtgaagt actcgactat gtggtggatg
     5341 aaggagtgca aatacatggc ggtaatggct tcagcgctga gtataatgct tcaagagcct
     5401 atcgtgacag ccgcattaac cgtatctatg aaggaacaaa tgagatcaat cggctgctca
     5461 cattagatat gacattgaag agagcaatgc agggccgttt gaacctgatg ggcccggcca
     5521 tggaagtgtc aaaagaactg atgagtattc ctgatttcgg cgcaacagat gaagctcctt
     5581 ttgccagcga aagaaagcta attgctaacc tgaagaaagc catcttgctt acagccggag
     5641 cagctgtaca aaagatgatg atgaagattg agaacgagca ggaggtactg atgaatattg
     5701 ccgatatggc tattgaaact tttaacgccg aaagtgcttt actccgggta atgaaaatgg
     5761 ttgatcagaa tggggaggcc gccagccagt accatctgga catgatgaga acttacctgt
     5821 atgatgcagc ggataaagta aacaagtccg gcaaagatgc ggttaactct tttgcagaag
     5881 gtgatgagca gcggatgatc ttaatgggcc tgaaacgttt tacaaaagca gaaccattca
     5941 acactaaaga agcaagaaga agagttgctg ataaactgat tgcagaaggc aggtattgtt
     6001 tctagttcca gctttcttta tcaaagtcaa gcatggctgt tcttaccggg gcagccattt
     6061 ttgtatcctt ataaatttct gatagttccc ggcgggttat ctgcacttct tttatttttt
     6121 taaccagttt tctttcataa ataattccgg cactcatcat cagtgcagaa actgtggctg
     6181 ccataaagta gaccaggaaa gtatctgcta catgcggcgt tttgaataca taatacataa
     6241 ggtaaataag gccaaaacat ccgtaagcaa ataataacga tcccgctatt aaagctttgc
     6301 ctgttgattt gccgtgctca acagccattt ttgcatgcct gacgaagaaa tgcaggcata
     6361 atgctgcaat cataataatg ccgggcccca gtatgattgt aatgctttca acattcaaac
     6421 ctcgtagggc aattacaact aactcaaata aaatgaatgc tgctacagcg atcttcagtc
     6481 tttttgtaaa aaggaaagaa gggctgaaat aagtcaggaa aagcatcatc agtggtgcat
     6541 ccagcaggtt gtttatcaac ccccagttat aaacgatttg tttactcata ggaatataac
     6601 cttcggtcat taggttataa ataaaaacag aggaatagta aacaactaaa gcaggaaagc
     6661 ttttataatt atacagccgc agggccagta tcagcgctat gggcagaaaa agggcaaagg
     6721 atgaaacaaa gcccattaca gaattccagg tcatagtcta ttggttttac aaggattgga
     6781 ttgggtggca aagcctggtt gcaatgcctt ctgcaaaatt aacgggggtt aaggtcgaaa
     6841 agtgttttat aataacggtt tatacgaaaa ttcagtaaaa gcgaaatact gtttcgtaat
     6901 tctacggaaa atatggttga gggtttttat gttatctttc tgtgatgata cgattaccat
     6961 tttttttagg attgttgatt gctgttcagg ggctttctca aacaacaggt aacaactttc
     7021 ttaccggcag gacaaacgga aaactgcctt atctcgagta tggattaggc tcagataggc
     7081 tgggcggagc gaaaatgacc ttcctggata ccggtgtagt cgtaaaagtt gtagacagca
     7141 ccgtaatcaa ttaccgggtg cagttatcag gaaatcattt tgcttatttg ccaaaggcca
     7201 actttaaaag ggacagctca gtaagtataa agccttatta cctgaccgga agctggctgg
     7261 taaacggtga tgataaatat gactacgtaa gcatagccct tgatgaaaaa ctaccatacc
     7321 ggagtatgca gcttatcaat ccttccaaac ttgtggttga tattttcgga gtcaccagca
     7381 ataccaactg gatcactcaa cgaacaagtg ttaaggaagt aaagaatgtt tatcatgagc
     7441 aaatagagga cgatgtgttc cggatgataa tagaactcaa tcattcacag cactggggat
     7501 atagtattta ttaccagggt aacaggctgt tcattaaaat aaaaagacag ccggatgagt
     7561 tgcaggtatc taaaatgaaa attgctgtag atgccggaca tggtggtgat aatagtggag
     7621 ccaaaggaat tacctcaggc attttggaga aacattacac attgatgatt gcaaaagaag
     7681 tagagaaaat gctgacacag gccggcgctg cagttttcat gacaagaaaa gaagatgtgt
     7741 cagtaggaat gacagaaagg atagagatgc tgaagaatga agaccccgat tttttgataa
     7801 gtatacattt aaactcgtca gtcagagaca gtgtaaaggg ggtaagcact tattatcgtt
     7861 atatcggctt caggccgttg acgcaatata ttcagaaagc catgctggaa atgggaatgg
     7921 ctgattttgg caacattggc agttttaatt tttcgttgaa cggacctaca gattatccta
     7981 actgtttact ggaagtggcc tttctcagca atagagaaga cgaagccctg atattggatc
     8041 ctgaatttca caaggccgtt gcaactcaag tactctgcgg catacaggaa tggctgaggg
     8101 cctgtgaaaa agacaaacct taatcttccg gcggaaatgt ttcgccgcga tggcaggttt
     8161 tacaggtgat ggtattcagg tattcgggtc tttctccggg ttggtaagga aaatattttt
     8221 ggttaatatc tatcgtcatc cgcatcattt cccttgcttt ttctttttta ggttcagcat
     8281 cggaagcata atccatatcg ccgggataga cttttttctt tacatggcag aactcgcatt
     8341 ttacacccag tgctttatta taggtctgca tgatactgtc cagtttttca tcgctgatat
     8401 ctatgggcag cactttcagg tttctttctt tttgttgtgg cgctttcacg gcagcaacgc
     8461 ctgatatcac aaatgcagtg agtaccagca aaaccaatat tttatttttg taacgcatat
     8521 tattactgac gtttttctat ttaattaggt ggcatacctt tttagttgcg gagccatcgt
     8581 tcaaaaaact cccatgttct cagcatctgg tcaatgcgct gccggttatt gccgctgcgg
     8641 gttaattcat gtgtggcatt gggatgccgt acatattcaa caggccttcc cagcaccttt
     8701 aaacttttat acagcatttc actttgtaca aaaccggtgc gccggtcatt gtcgccatga
     8761 aaaatgatgt aaggcgtttt aatattttgt acataattaa tcggcgattc tctttccagt
     8821 gttgcctttg cagaagcatc ccagggataa cctccgaaat aattaggtac taatcgccag
     8881 gcattgcctt caccaaaaaa agtagcgaga tcatacacac ctcgctggga gcaagccgct
     8941 gcaaatcgct ggtcatgggc aatgatccag gccaccaaat aaccggcata ggatccgccc
     9001 gttaccagca gtttggaagt atcagcccag ccttcagcaa ttgttttgtc cagtgcagtt
     9061 aatacatctt tggttggccc ggctccccag tcattgatat ttccccttag aaaatttatc
     9121 ccata
//
LOCUS       DAORLS010000112         9076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4136_length_9076_cov_1.953885, whole genome shotgun sequence.
ACCESSION   DAORLS010000112 DAORLS010000000
VERSION     DAORLS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4136_length_9076_cov_1.953885"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1315
                     /locus_tag="PK977_09695"
     CDS             158..1315
                     /locus_tag="PK977_09695"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRF18432.1"
                     /translation="MKAKSIKGNSADEIRAALQQSMADDFKPTLAIVFISVKQDRAVI
                     SDLLFHHNIDVFGCTSCGEFINGHQSEGETAILLLDVCRDYYFTLIEEVENAGVEKAA
                     SLLAAKALHHFKSPTFLLSCNGVYENGQYFDGDTLVKTLVRELGDGCVFFGGMAGDDW
                     GIMNSYVFTHQNESGNAIAALVFDAEKISLHGMATHGWKPLGIARKVTKSGGNKVYSI
                     DGKPAAELYLKYLGMADKTGDESFDIFKDLSVHYPFIAKRKDGETIIKSPRSIDKQSG
                     ALIMDIEMEEGAEFHFTTPPDFEISEEIISEATALKNSLQGKPDALLIFSCAGRPPVL
                     GPLTSVENEGLAKVWQTPMAGFYTYGEFGRTKNGKQHFHSGMCCWVTITEK"
     gene            1335..1646
                     /locus_tag="PK977_09700"
     CDS             1335..1646
                     /locus_tag="PK977_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HRF18433.1"
                     /translation="MIKATLLYGHPTDPEAFEKYYAETHLPLAAKMQGVNRLELTKFI
                     SAPDGSKAAYYRQADLFFSSPGQLQQTMSSPEGLAAAADLANFATGGVTMIIGTVENS
                     F"
     gene            1675..>2198
                     /locus_tag="PK977_09705"
     CDS             1675..>2198
                     /locus_tag="PK977_09705"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HRF18434.1"
                     /translation="MVLIQKQLRNWLVFFVIIQFGIMSLNAQTGFHKEIKIYADSLYH
                     SFSKLEKRNALLSFSDTARLKWNNLPLGLRARAGISIGNMNNDQRRLVHRILSVSFSS
                     QGYLKATGIMHLDNLINGYYDSLYLQKKFSDTVYERIKALQWSHKNFFFAFFGSPQDK
                     NWGYKLEGHHLSVN"
     assembly_gap    2199..2248
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2249..2779
                     /locus_tag="PK977_09710"
     CDS             <2249..2779
                     /locus_tag="PK977_09710"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HRF18435.1"
                     /translation="GTDPAEYMISEYAGWRVLGQEQDLGLRFINMLTPELRKKATKDT
                     EVPADIITGAESGKRLIDYWGIKGSELTAIQKTALEHIIREFVFNLEYDKAIVEYDKI
                     KKAGIDNIWFGWIGAYNEEKQHYFVINGPTFLIEFDDRSGGPNREANHIHAIWREKGN
                     EFGEDVLRQHYLKEKH"
     gene            2787..>3196
                     /locus_tag="PK977_09715"
     CDS             2787..>3196
                     /locus_tag="PK977_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRF18436.1"
                     /translation="MKAITIKGKSSLDIQSALEQSMADGFNPTLAVVFISLKQDREAI
                     CSLLNNHRIQIFGTTTSGEFINSEISEGGIAIMLLDLDPAFFKMVFLETNAGSAYEVS
                     KQLGETGRTSFDQAAFIVASGWLHTDGEQIIRGI"
     assembly_gap    3197..3206
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3207..3942
                     /locus_tag="PK977_09720"
     CDS             <3207..3942
                     /locus_tag="PK977_09720"
                     /inference="COORDINATES: protein motif:HMM:NF021926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HRF18437.1"
                     /translation="RCAGVWRLGRSGGMAGDDLALSGPVVFTYGKSSITGLVALIFDS
                     DKVEVTGIATCGWKPIGITKTVTKSEGNVVYTIDDQPAFDLVIKYLGLNTDEMNAINN
                     TVVNLGAYYPLQLERDNAPPVMRTAMLGNKEDRSLICAGNVPQGAKVRFSLPPDFDVI
                     DTVVEECTGLKTDTQGNADAVIMFSCISRYLSFGVMTSEEIERVMQVWNAPFIGYFSY
                     GEFGKSKRGKHEFHNNTCCVVVLKEK"
     assembly_gap    4016..4025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4026..4486
                     /locus_tag="PK977_09725"
     CDS             <4026..4486
                     /locus_tag="PK977_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRF18438.1"
                     /translation="KAQLAGMYKLYIAENADSNGVWHEDPWTKGGTGYIVYDGLGHMA
                     VHITRKGYNDYNWLPEEQSLRDEYINQKLDSMSTDELKDAVRAFASSYVYAGNYNIED
                     TADIVQHHRISTSIHSPVGSTVRRAYSFSGDTIILHVLNGNRRLKWIKQP"
     assembly_gap    4501..4620
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4621..5641
                     /locus_tag="PK977_09730"
     CDS             <4621..5641
                     /locus_tag="PK977_09730"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRF18439.1"
                     /translation="RQAINELLDKEGITIFGCTTNGEFIDENPEKASSAILLLDINKN
                     HFQIYFEEFQENKYRQVAKSIAQNAKSHFSVPAFIIGISNAAADGEEVLRGIEEIAGP
                     EVNAFGGAAGDDYSFSETFVFTNGKDSNNAMVCIALNEAKVEVKGIATCGWKAVGTEK
                     VVTKSEGNHVYTVEDIPVLDLTAKYGGIDDPTPENNNLLVEIAANFPLQLQREKGDPV
                     MRPGLVVDWNDRSYYTSGSVPQGSKVRFSLPPDFDVMEKVVKGVQNLKNTIMPEADAL
                     VVFSCAGRILSLGPLMSQELDGIKKVWNVPMAGMFSNAELGRATGGNLEMHNLTTCCI
                     ALKEK"
     gene            5642..6676
                     /locus_tag="PK977_09735"
     CDS             5642..6676
                     /locus_tag="PK977_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF18440.1"
                     /translation="MNPQFQYILDLIQQSQLSDADRTALENAVKEADKQYEIINFKLD
                     RTEKVKRTTAILLEETIAELELKRKAVEESNDALQKSLEELKAAQQQLIQSEKMASLG
                     ELTAGIAHEIQNPLNFVNNFSDVSKELLDEMKVELEKGNMEDAREIANDVIQNLEKIL
                     HHGKRADSIVKGMLQHSRSGSGEKELTDLNNLVDECLRLSYHGMRAKDKSFNAKTDSS
                     LDAGLPQIKIVSQDIGRVLLNLFTNAFYSVLQKKKNADGNYEPLVSAKTILESGKVKI
                     IVEDNGIGIPQRVIEKIFQPFFTTKPTGEGTGLGLSMSYDIITKGHNGELQVETKEGE
                     FARFTIILPI"
     gene            6711..>7048
                     /locus_tag="PK977_09740"
     CDS             6711..>7048
                     /locus_tag="PK977_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF18441.1"
                     /translation="MKILVVDDERDVQTLFEQRFRKEMKSGEMQFNFAFSGEEALQFL
                     TDHNQEAVLILSDINMPGMSGLELLKRIKQEHHEPPPVVIPTLPAITPPPLPAPTALT
                     PNSSRAFRKPP"
     assembly_gap    7049..7058
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7059..8138
                     /locus_tag="PK977_09745"
     CDS             <7059..8138
                     /locus_tag="PK977_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF18442.1"
                     /translation="AGRFYSIKRKIKSNTLMAKILVVDDEADLETLIRQKFRQKIREN
                     QYEFVFAMNGVKALEQLEQHQDVDVVLSDINMPEMDGFTLLSRLNEQNSLLKSVIVSA
                     YGDMENIRTAMNRGAFDFITKPVNFEDLETTMQKTLRHVEQMKETMRAIKENNILRMY
                     VDETVLNFMGGKEFEKSLLENETVEATVAFIDICGFTAISENEKPDTVVKLLNNYFDV
                     MVKEIIAQKGLVDKFIGDCVMAVFKGEYHLDRAIDASLAIRNKIAALPATEGFTPKVS
                     IGINSGEMISGNIGSGTLRRLDYTVIGDTVNTAQRLQTVAGPNQIVISESCYEKIKQS
                     FSCNKIGEVSLKNKSNPVMIYEVLD"
     gene            8150..>8458
                     /locus_tag="PK977_09750"
     CDS             8150..>8458
                     /locus_tag="PK977_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF18443.1"
                     /translation="MQYQLVYNFLMPKLEEELPEYYTYHNAAHTKSVIAAAEYLGTLE
                     KISEEEMLLLKTAALFHDAGFLQQADGHEEISCILARDHLPEFGYSEKHIGEISRIIM
                     A"
     assembly_gap    8459..8470
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8471..>8800
                     /locus_tag="PK977_09755"
     CDS             <8471..>8800
                     /locus_tag="PK977_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18444.1"
                     /translation="TRLPQTPVNHLGQILCDADLFYIGKDGYAANAEKLFQEFKYRGF
                     VKTEAEWQLMQVEFLSNHKFFTKSAISELEAAKQQIIKELTSGVEHSLASHHEDSAGQ
                     LIQDLVL"
     assembly_gap    8801..8833
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8834..>9076
                     /locus_tag="PK977_09760"
     CDS             <8834..>9076
                     /locus_tag="PK977_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRF18445.1"
                     /translation="LRKSNSPYFRDFFDGGVTGISLLVHEVYGFNLGLVILLLNLPLI
                     IVSHFTIGKRFALRTLLSVILLGVALLLIPNYAMTA"
BASE COUNT         2773 a         1621 c         2070 g         2367 t
ORIGIN      
        1 ggttgtttgg aacatttaaa tacaagccgc cagagcaaat taattttggt ttaaaagaat
       61 ttgaagagga cagaaaacaa accttctggt atcttatccg aagcccattc attagcatgg
      121 gaagaataac ttccggtgat ttgaaaaagg aaaagaaatg aaagcaaaat caataaaggg
      181 aaattccgca gatgaaatca gggctgcact acagcaaagt atggcagatg actttaagcc
      241 aacactggct attgtattta tttctgtcaa acaggacagg gcagtcatat ccgatttatt
      301 atttcatcat aatatagatg tattcggttg tacctcctgt ggtgaattta tcaatggcca
      361 tcaaagcgaa ggtgaaacgg caatactttt actggatgta tgccgggatt attattttac
      421 cttaattgaa gaggtagaaa atgccggtgt ggaaaaagcg gcttcactcc ttgccgcaaa
      481 agcgcttcat cattttaaat ccccgacatt tttattgagc tgtaatggcg tctatgaaaa
      541 tggtcaatat tttgatggcg atacactggt aaaaacattg gtaagggaac tgggtgatgg
      601 gtgtgttttc tttggcggaa tggccggtga tgactgggga attatgaaca gctatgtatt
      661 tactcatcaa aatgaatccg gaaatgccat tgccgctttg gtttttgatg ctgaaaaaat
      721 ttctttacat gggatggcta cccacggttg gaagccgttg ggcattgcca ggaaggtaac
      781 aaaaagcgga ggtaataaag tttacagcat tgatggaaaa cctgctgcag agttgtattt
      841 aaaatatctg ggtatggcag ataaaaccgg ggatgaaagt tttgatatct ttaaagacct
      901 gtctgtccat taccctttta ttgctaaacg taaagacgga gaaactatta ttaaatcgcc
      961 ccggtcgata gataagcaat ctggcgcttt gatcatggat atagaaatgg aagaaggtgc
     1021 cgagtttcac tttacaaccc cgcctgattt tgaaatttca gaagaaatta tttcagaagc
     1081 aacagctctc aaaaattcac ttcagggcaa acctgatgcc ctgctcattt tctcctgtgc
     1141 cggaaggcca cccgttttag ggccgttgac ttcggtggaa aatgaaggcc tggcgaaagt
     1201 atggcagacg cctatggccg gcttttatac ctatggtgag tttggaagaa caaaaaacgg
     1261 gaagcagcat tttcattcgg gtatgtgctg ctgggttaca ataaccgaaa aataaaaatt
     1321 gaaaaccagc aaaaatgata aaagcaacat tactctacgg tcatcccaca gacccggaag
     1381 catttgagaa gtattatgca gaaacacatt tgccccttgc agctaaaatg cagggtgtaa
     1441 acaggttgga gctgacaaaa ttcatttcag ctcctgatgg aagtaaagca gcctattatc
     1501 gacaggctga tttatttttt tcaagccccg gacaattgca gcaaaccatg agctcacccg
     1561 aaggactggc tgctgctgct gacctggcca attttgctac cggcggagtt acaatgatta
     1621 ttggcaccgt tgaaaattca ttttaaccaa acagaaatat tttcaattat tattatggtt
     1681 ctaatacaaa agcagttaag aaattggtta gtcttttttg tcatcatcca gtttgggata
     1741 atgtctttga atgcacaaac aggatttcat aaagagataa agatttatgc agactcattg
     1801 tatcattcat tcagcaagtt agaaaagaga aatgcccttc tttctttttc ggatactgcc
     1861 aggttaaagt ggaataatct tccgttgggt ttaagagcaa gagctggtat cagcattggt
     1921 aatatgaata atgaccagcg cagactggtt catcgtattc tttctgtttc attcagctct
     1981 cagggttact tgaaggctac cggcatcatg catctggata atttaattaa cggttattat
     2041 gacagccttt atttacaaaa aaaatttagt gatactgttt acgaaaggat aaaagcattg
     2101 cagtggtcgc ataagaattt cttttttgcc ttttttggca gcccacagga caagaactgg
     2161 gggtataaac tggaggggca tcacttatcg gtcaacttnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tactgacccg gctgaataca tgatatctga
     2281 atatgcaggc tggcgggtgc tgggccagga acaagatctt ggccttcgtt tcatcaacat
     2341 gcttactcct gaactcagga agaaagctac gaaggataca gaagtgccgg cagacattat
     2401 caccggagca gaaagcggca agcggttgat tgattactgg ggaataaaag gatctgagct
     2461 gacagccata caaaaaactg cactggagca tatcatcagg gaatttgttt ttaacctcga
     2521 gtatgataaa gcaattgttg agtatgataa aataaaaaaa gcagggattg ataatatttg
     2581 gtttggttgg attggtgcct acaacgagga aaagcagcat tatttcgtta tcaacgggcc
     2641 tacctttctg attgagtttg atgacaggag cggtggcccc aacagggagg caaatcatat
     2701 ccatgccatc tggcgggaaa aaggtaacga attcggcgaa gatgttttga gacagcacta
     2761 cctcaaagaa aaacactaaa aggttcatga aagctataac aataaaagga aagtcgtcgc
     2821 ttgacattca gtctgctttg gagcaaagta tggctgatgg tttcaatccc acattggctg
     2881 ttgttttcat ttcactaaag caggatagag aagctatttg ctcattactt aacaatcaca
     2941 gaatacaaat ttttggtacc accacttctg gcgaattcat aaactccgag atcagtgaag
     3001 ggggcattgc tattatgttg cttgacctgg atcctgcatt tttcaaaatg gtttttttag
     3061 aaacaaatgc tggttcggct tatgaagttt caaaacaatt aggtgaaaca gggagaacca
     3121 gttttgacca ggctgcgttc atagtagctt ccggctggct gcatacggat ggagaacaga
     3181 ttataagggg aattgannnn nnnnnnccgc tgcgctggtg tatggcggct tggtcgcagc
     3241 ggcggcatgg ctggcgatga cctggcatta agcgggccag tcgtctttac atacggcaaa
     3301 agcagtatta caggactggt agctctcata tttgattcag ataaagtgga agttactgga
     3361 attgctactt gtggatggaa gccaatcggt attactaaaa cggtgaccaa aagtgagggt
     3421 aatgttgttt ataccattga tgatcagcct gcatttgatc ttgttatcaa atatcttgga
     3481 ttgaatacag atgaaatgaa tgccattaac aatacagtgg tcaacctcgg tgcttattat
     3541 ccgctgcaat tggaaaggga taatgcgcca ccggttatgc gtactgctat gttgggtaat
     3601 aaagaagaca ggtcactgat ctgcgctggc aacgtaccgc aaggggctaa agtccgcttt
     3661 tcacttcccc cggattttga tgtaattgat acggtggttg aagaatgtac aggtttgaaa
     3721 actgatacac aaggtaatgc agatgcagtt attatgtttt cctgtattag ccgttatctg
     3781 tcatttggcg taatgacgag tgaagaaatt gagagagtga tgcaagtctg gaatgcacct
     3841 ttcataggtt atttcagtta tggcgaattt ggaaaatcaa aaagaggcaa acatgaattt
     3901 cataacaata catgttgtgt ggtggtatta aaagaaaaat aaattcaata agaatgaaaa
     3961 ttgttacact cgttctcttg tcaattattt tattttcttg caagagaaaa aaggannnnn
     4021 nnnnngtaaa gcacaactgg caggcatgta taagctctat attgctgaaa atgctgactc
     4081 aaacggagtg tggcatgagg acccatggac caaaggcggc acaggatata ttgtttatga
     4141 tggattagga catatggccg ttcatatcac ccgcaaagga tataacgatt acaattggct
     4201 gcccgaagaa caaagcttgc gtgatgaata tatcaaccag aaactggata gtatgtcaac
     4261 cgatgaactg aaggatgcag tccgggcatt tgcttccagt tatgtatatg ccggtaatta
     4321 caatattgaa gacacggccg atatagtgca gcatcatcgc atctccactt ctattcattc
     4381 accggtaggc agtactgtaa gaagagcata ctcattcagc ggcgatacta ttatacttca
     4441 tgtattaaac gggaaccgta gattgaaatg gataaagcaa ccataaaata atttcatgaa
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 cagacaagcc attaacgaat tattggataa agagggtata accatattcg gctgtaccac
     4681 taatggtgaa ttcattgatg aaaacccgga gaaagcatca tctgccatcc tgttgctgga
     4741 tataaataaa aaccattttc agatttattt cgaagaattc caagagaata aatacaggca
     4801 agtcgcaaaa agtattgcac aaaatgccaa atctcatttt tctgtacctg cattcattat
     4861 cggtatcagc aacgcagcag cagatggtga agaggtttta cgtggaatag aagaaattgc
     4921 cggaccggaa gtaaatgcat ttggcggagc cgccggcgat gattattcat tttcggagac
     4981 gtttgttttt accaatggta aagacagtaa taatgccatg gtatgcattg cgcttaatga
     5041 agcaaaagtt gaagtaaaag gtattgctac ctgcggatgg aaagcagtag gaacagaaaa
     5101 agtagtgacc aaaagtgagg gcaaccatgt gtacacggta gaagatattc cggtgcttga
     5161 cctgacagct aaatatggcg ggatagatga cccaaccccg gagaacaaca atttactggt
     5221 ggaaattgct gccaacttcc ctttgcaatt gcagcgggaa aaaggcgatc cggtgatgcg
     5281 gcctggcctg gtggttgact ggaatgaccg ttcttattat accagcgggt cagtgccgca
     5341 gggatcaaaa gtccggtttt cattacctcc tgattttgat gtaatggaaa aagtggtgaa
     5401 aggtgttcaa aatctgaaaa acacaataat gccggaagcg gatgcactgg tggtattcag
     5461 ttgtgcagga agaatattat cattaggtcc gttaatgtcg caggagttgg atggaataaa
     5521 aaaggtgtgg aatgtgccaa tggctggaat gttttccaat gcagaattgg gaagagcaac
     5581 aggtggaaac ctggaaatgc ataatctcac cacttgttgt attgcattaa aagaaaagta
     5641 aatgaatcct caatttcaat acatcctcga tttaattcag caatcgcaac taagcgatgc
     5701 tgatagaact gctttggaaa acgcagtaaa ggaggcagac aagcaatatg aaataataaa
     5761 ctttaaactc gaccgtaccg aaaaagtaaa acgtaccaca gccattttgc ttgaggaaac
     5821 catcgctgaa ctggagttga aaagaaaagc tgtggaagaa tcaaatgatg cattacaaaa
     5881 atcactggaa gagctgaagg cagcacaaca gcaactgata caatcagaga aaatggccag
     5941 cctcggtgaa ctcactgccg gtattgccca tgaaattcag aacccgttga atttcgtcaa
     6001 taatttttct gatgtaagta aagaattact ggatgagatg aaggtggaac tggaaaaagg
     6061 aaacatggag gatgccaggg agatagctaa tgatgttatt cagaacctgg agaaaatatt
     6121 gcaccatggc aaacgggcgg attctatcgt aaagggaatg ctgcagcaca gccgtagtgg
     6181 cagcggtgaa aaagagttga ccgatctgaa taacctggta gatgaatgtc tgcgcctgag
     6241 ctatcatggt atgcgggcca aagacaaaag ttttaatgcc aaaaccgata gttcgctgga
     6301 tgccggcctg ccacagataa aaatagtttc ccaggacata ggcagggtac tgctcaatct
     6361 ttttaccaat gctttttatt ctgtgctgca gaaaaaaaag aatgctgatg gtaattacga
     6421 acctcttgtt tcagctaaaa caatattgga atcagggaag gtgaaaataa ttgtagaaga
     6481 taacggcata ggtatacctc aaagagtaat tgaaaaaata tttcaaccct tctttaccac
     6541 caaacctact ggtgaaggaa ccggtttggg tcttagtatg agttatgata tcattaccaa
     6601 aggtcataat ggtgagttgc aggtggaaac aaaagaagga gaatttgccc ggttcaccat
     6661 tattttacct atataaacct tattttcaat tactagtaaa ccaccacagc atgaagatct
     6721 tagttgtaga tgacgagagg gatgtccaaa cacttttcga gcagcgtttt cgcaaagaaa
     6781 tgaaaagcgg ggagatgcaa tttaactttg ctttctcagg ggaagaagcc ttacaatttc
     6841 tgacagatca taaccaggag gctgtactaa ttttatctga tatcaatatg ccgggtatga
     6901 gcggactgga attattgaaa cgcatcaaac aggagcacca cgagccgccg ccggtggtga
     6961 ttccgacgct gcccgccatc accccgccgc ccctgcccgc gccgacggcg ctgaccccga
     7021 actcgtcacg cgccttcagg aaaccaccnn nnnnnnnngc cggtagattt tacagtatta
     7081 aaagaaaaat taaaagcaat acactgatgg caaagatcct tgtagtagat gatgaggccg
     7141 atctggaaac attgatacgg cagaaattca ggcaaaagat acgggagaac cagtatgagt
     7201 ttgtttttgc catgaatggg gtaaaggcgc tggaacaact ggaacagcac caggatgtgg
     7261 atgtggtact cagtgacata aacatgccgg agatggatgg ctttacctta ctatctagac
     7321 tgaacgaaca aaacagtttg ctgaaatctg tcattgtttc agcctatggc gatatggaaa
     7381 atatccgcac tgctatgaac cgcggtgctt ttgattttat taccaagccg gtgaattttg
     7441 aggacctgga aactaccatg caaaaaaccc tgcggcatgt agagcagatg aaagaaacca
     7501 tgagggctat caaagaaaat aatattctgc ggatgtatgt ggatgaaaca gtgctgaatt
     7561 ttatgggtgg aaaagaattc gaaaaatcac tgctggagaa tgaaacagta gaagctactg
     7621 ttgcatttat tgatatttgc ggctttacgg cgatcagtga aaacgaaaaa cctgacacag
     7681 tcgtgaaatt gctgaacaat tattttgatg taatggtgaa agagatcatt gcacaaaaag
     7741 gactggtgga taaattcata ggcgattgtg tgatggcagt tttcaaaggt gagtatcatc
     7801 ttgacagggc gattgatgca agtcttgcta ttcggaataa gattgctgcc cttcctgcta
     7861 ctgagggctt caccccaaag gtttccatag gtatcaatag cggtgaaatg atctccggta
     7921 atataggctc gggtacatta agacgcctgg attatacggt gatcggtgat acggttaata
     7981 cagcccagcg gttgcagact gttgcgggtc ctaaccagat cgttatttca gaaagctgct
     8041 atgaaaagat caagcaatct ttcagttgca ataaaattgg ggaagtgagc ctgaagaata
     8101 aatcaaaccc ggtcatgatc tacgaagtat tggattaaag aaaaaggaaa tgcagtacca
     8161 gctagtttat aattttctaa tgcctaaact ggaagaagag cttccggaat attatactta
     8221 tcataatgca gcgcatacca aaagcgttat tgcggcagcg gaataccttg ggactttgga
     8281 aaaaataagt gaagaggaaa tgctattgtt gaaaactgct gctttatttc atgacgccgg
     8341 ctttctgcaa caggcggacg gacacgaaga gatttcctgc attttggcga gagaccatct
     8401 gcctgaattt ggatacagtg aaaagcatat cggggaaata tcccgtatca tcatggctnn
     8461 nnnnnnnnnn ctacccgttt accacaaaca cctgtgaacc atctcggtca gatactttgc
     8521 gatgctgatt tattttatat cgggaaagat ggctatgccg ctaatgcaga aaaattattc
     8581 caggaattca aataccgggg ctttgtgaaa acagaagccg agtggcaact gatgcaggta
     8641 gagtttcttt ccaatcataa attttttacg aagtcggcta tttcagaact ggaggcggcc
     8701 aagcagcaaa ttatcaaaga acttacttct ggagtggagc acagtcttgc ttcccaccac
     8761 gaagattcag caggccaact gatccaggac ctggtactga nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnntattacg gaaaagcaat tctccttatt tccgtgattt ttttgatggt
     8881 ggtgttaccg gaatctctct actggtgcat gaagtgtatg gcttcaacct gggactggtt
     8941 atattgttgc tcaaccttcc cttgatcatt gtaagccatt ttacgattgg aaaacgtttt
     9001 gccctccgta cattgttgag tgtcattttg ttgggagttg ccctgttgct gattcctaat
     9061 tatgctatga cagccg
//
LOCUS       DAORLS010000113         9047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4153_length_9047_cov_1.836966, whole genome shotgun sequence.
ACCESSION   DAORLS010000113 DAORLS010000000
VERSION     DAORLS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4153_length_9047_cov_1.836966"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>662
                     /locus_tag="PK977_09765"
     CDS             <1..>662
                     /locus_tag="PK977_09765"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRF18446.1"
                     /translation="DKKTFELYQHGATIGTFQFESAGMQKYLRELKPDKFDDLIAMNA
                     LYRPGPLAYIPNFIDRKHGREAITYDLPEMEEYLKDTYGITVYQEQVMLLAQKLAGFS
                     KGDADVLRKAMGKKQKTVLDKMKAQFISGATEKLFPPEKLEKIWTDWEAFAQYAFNKS
                     HSTCYAYVAYQTAYLKAHYPAEYMAAVLNNAGSIEKITFFMEECKRMGIKVLGPDINE
                     SL"
     assembly_gap    663..696
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <697..>1512
                     /locus_tag="PK977_09770"
     CDS             <697..>1512
                     /locus_tag="PK977_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRF18447.1"
                     /translation="RFGLGGLKGVGEAAVESIISERQKNGSFVSLFDFIKRINQRTVN
                     KKTLESLAYAGGFDCFTEYHRAQYFVVPENEMINGLEKIIKYGQVIASQNANTANTLF
                     GDLPATMEIPPPKLADCPHWPLTVQLDHEKEVTGMFLSGHPLDHYRFEMKHYGVTPIA
                     DFNEFKEAIKMHPNPGRPFRIVGLVADAQHRIARSGNKYGNFVIEDYSGKTEIVLFSE
                     DYLRLTPVLQQGTTVFITGYFKQRYNRDEFEFKVNSVSLAETMKKNLTKQVTI"
     assembly_gap    1513..1522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1523..1741
                     /locus_tag="PK977_09775"
     CDS             <1523..1741
                     /locus_tag="PK977_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18448.1"
                     /translation="VHPQDITKDMVNFVEKNLQNFPGKSTLKFNISEPKNRMRISLVT
                     MNSGFEMNEELIQFLEKSSELDVQVLTV"
     gene            1886..>2177
                     /gene="trxA"
                     /locus_tag="PK977_09780"
     CDS             1886..>2177
                     /gene="trxA"
                     /locus_tag="PK977_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRF18449.1"
                     /translation="MAREFTDANFQSDVLASDKLTVVDFWAEWCGPCRAIGPVIEELS
                     KEYDGKVNVGKVNVDNNPNISMNYGITSIPAILFIQNGQVIDKLVGAQPKANF"
     assembly_gap    2178..2272
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2273..>2929)
                     /locus_tag="PK977_09785"
     CDS             complement(<2273..>2929)
                     /locus_tag="PK977_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF18450.1"
                     /translation="AFKLFAKYKMALFFIFSMFLGAALQLTNMYGDTFLDDFKKVPEY
                     GPESFVVKYSTIIMSISQISETVFILTIPFFLRKFGIKKVMLFSMIAWVLRFGLFAYG
                     NPADGLWMIILSCIVYGMAFDFFNISGSLFVETTTDSSIRSSAQGLFMMMTNGVGAFL
                     GSKVSGYLIDKYFTVNGGDKDWHNIWLAFAAYAAVIAVLFLVLFKHKHNPKELETVHH
                     "
     assembly_gap    2930..2997
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2998..3218)
                     /locus_tag="PK977_09790"
     CDS             complement(<2998..3218)
                     /locus_tag="PK977_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF18451.1"
                     /translation="MPTISLSNSISYTILKKNAFDVVKVFPPIRVWGTIGFIVAMWTT
                     NLTDSKANTNQFLIGGVAAILLGIYAFTLP"
     assembly_gap    3262..3313
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3314..3555)
                     /locus_tag="PK977_09795"
     CDS             complement(<3314..3555)
                     /locus_tag="PK977_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF18452.1"
                     /translation="MNIKIRLTILSFFQFFVWGAWLITIATYFFTNNMGSGAQFGAIF
                     STLALSSLFMPALTGIIADKWMNAERLYGILHILYGA"
     gene            3677..>3945
                     /locus_tag="PK977_09800"
     CDS             3677..>3945
                     /locus_tag="PK977_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HRF18453.1"
                     /translation="MITTTDISPELKAIADKVSAGHRISDEDCLYLFEHASLGFVGSL
                     ANAIKERLHGDKVFFNRNFHIEPTNVCVFSCNFCSYSRLYAKREDG"
     assembly_gap    3946..4014
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4015..4830
                     /locus_tag="PK977_09805"
     CDS             <4015..4830
                     /locus_tag="PK977_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRF18454.1"
                     /translation="ITEVHIVGGVHPKMDLQFFADLLKAIKAHRPDLHIKAFTAVEYD
                     YMFRKAKVSVEEGLKFLIEAGLDSIPGGGAEIFHPEIREKICADKVDADGWLLIHETA
                     HKLGLPSNATMLYGHIEKFEHRIDHMRWLRELQDRTKGFNTFIPLKFRNKDNDMSNVP
                     ETSVVEDMKMYAIARIYLDNFPHLKAYWPMLGRQNAQLTLSFGVDDIDGTIDDTTKIY
                     SMAGSEEQNPAMSTTELVNLIKQVKRVPVERDTVYNEIKNYSGTVNEPNPALN"
     gene            4827..5510
                     /locus_tag="PK977_09810"
     CDS             4827..5510
                     /locus_tag="PK977_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRF18455.1"
                     /translation="MTRLSLLLIFILLNIAGKSFAQQKYTITYENFSFYIFDSTGNPV
                     KPAWDKKITTQIVINDQQSYYYTVFNGNKPIEPGKVLGKNFLPHTNYVHLQNKILVSQ
                     FGDKYRVREPIIDFKWQLLTGKKIVAGFECKMAYCIYQGDSLVASYSEKLPVLFGPLT
                     YTGLPGTILETDHYRKENVYRTTALKVENTANPIVEPKDGKLISWEQWKKIIANRRSD
                     NGVIRVVRW"
     gene            complement(5542..6042)
                     /locus_tag="PK977_09815"
     CDS             complement(5542..6042)
                     /locus_tag="PK977_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18456.1"
                     /translation="MATKKKAKKLAKPAAKKKAVAKKKVKVVKKKAAKKPVKAKAKSK
                     KAAKKVVRRPAKPVAKKKAKVVKKKAVKKVAKKKVVAKKPAPKKKAPVKKVKPAPVAV
                     PPPVMEETPVLVEEITETFVTPDGTLEVTETILTFEEEPEEDNGFAEDDLPDAEEEND
                     EEGPVM"
     gene            6183..6656
                     /locus_tag="PK977_09820"
     CDS             6183..6656
                     /locus_tag="PK977_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRF18457.1"
                     /translation="MAEDLQIIRGSKTVQYESLVPQIKGLLEGETDLVANLANVAAAL
                     KEQFGWFWVGFYLVKDNELVLGPFQGPVACTRIRKGRGVCGSSWEQQKTLIVPDVEKF
                     PGHIACSSLSKSEIVVPVIRNNEVLGVLDVDSDQYDQFDSTDQQYLEQIVSLIQF"
     gene            6660..>6883
                     /locus_tag="PK977_09825"
     CDS             6660..>6883
                     /locus_tag="PK977_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRF18458.1"
                     /translation="MLKTLSIIVTGKVQGVWYRQSAKEKATELGVTGNVRNQPDGSVA
                     IIATGLAKQLDQFIEWCRQGPPRALVTNVEV"
     assembly_gap    6884..6929
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6948..7310)
                     /locus_tag="PK977_09830"
     CDS             complement(6948..7310)
                     /locus_tag="PK977_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18459.1"
                     /translation="MTRYFIFTTMLFALLMAKPITTLAGTQQQSGHIATNHHSDDPGK
                     AANTGHLPMGAKQHPGGEKAHSHVPSWDELAHIHHFHKNRLKKVRRHYSKCVFFSKLL
                     LIICHAAILFVSYLHVTH"
     assembly_gap    7562..7571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7609..>7868
                     /locus_tag="PK977_09835"
     CDS             7609..>7868
                     /locus_tag="PK977_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18460.1"
                     /translation="MLLLLAITAIVYLLLRPKPGLTIEEPRPQMNINNCFTITRDDMV
                     NSWLGPNWNVKGDPNYISGIMFEAIDTDPNNIKIVAYPVDNS"
     assembly_gap    7869..7961
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7962..8204
                     /locus_tag="PK977_09840"
     CDS             <7962..8204
                     /locus_tag="PK977_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18461.1"
                     /translation="NSLKAQKTRYDFASSDIDPFGDLIHFNFMRLIPKPDATNPANLS
                     FVVEYVKIKGGSETVLSRGDTKPCPPYCPTDPDLIK"
     assembly_gap    8384..8403
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8404..8880
                     /locus_tag="PK977_09845"
     CDS             <8404..8880
                     /locus_tag="PK977_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18462.1"
                     /translation="LRNNTIFYNLHSIARVSFFSWYIITIRKYYRPQLYKILLAAYFV
                     FLAFNFIFNDSITVYNNDLATAETITLLVLCIFFFLRSIQDDSEVNWLKHPAFLFCAG
                     IFLYESVSFFINLFFYKMAEKNLSFGSLMLDIQNLIFLILCLMISMGLYSNRTKNE"
BASE COUNT         2613 a         1754 c         1756 g         2427 t
ORIGIN      
        1 atgataaaaa gacatttgaa ctataccagc atggagcaac tattggcact ttccagtttg
       61 aaagtgccgg tatgcagaaa tacctccggg aactgaagcc ggataaattc gatgacctta
      121 tcgccatgaa tgccttatac cggcccggcc ctctggcata tattcccaat ttcatcgatc
      181 gtaaacatgg ccgggaagcc attacttatg acttgccaga aatggaagaa tacctgaaag
      241 atacatatgg tataaccgtg taccaggagc aggtgatgtt gctggcccaa aagttggccg
      301 gcttcagtaa aggagatgcc gatgtgcttc gtaaagcaat gggtaaaaag caaaagacgg
      361 tactcgataa aatgaaggca cagtttatat ccggtgcaac tgaaaaactc tttcccccgg
      421 agaaattaga aaaaatatgg actgactggg aagcctttgc ccaatatgca ttcaataaat
      481 cacactccac ttgttatgcc tatgtggctt accagacagc atacctgaaa gcccattatc
      541 ctgcggaata catggctgct gtactgaaca acgcaggcag cattgaaaag atcactttct
      601 tcatggaaga atgtaagcgg atgggtataa aagtgctggg accggatatc aatgaatcat
      661 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgt tttggtttag gtggcttaaa
      721 aggtgtgggt gaagcagctg tagaaagcat tatttctgag agacagaaga acggttcttt
      781 tgtcagcctt tttgatttca tcaaacgtat taaccagcgt accgtcaata aaaaaacact
      841 ggaaagcctt gcctatgcag gtggttttga ctgcttcaca gaatatcacc gggcgcaata
      901 tttcgttgtc ccggaaaatg aaatgatcaa tggcctggag aaaataatca aatacgggca
      961 ggttattgct tcgcaaaatg ccaatacggc caatacactt ttcggcgatt taccggcaac
     1021 catggagata ccaccgccca aattggccga ttgcccacac tggccgttga cagtacaatt
     1081 ggatcatgaa aaagaagtga ccggtatgtt cctgagtggc catccgctgg accactaccg
     1141 gtttgaaatg aaacattatg gcgttacccc cattgctgat ttcaatgaat tcaaggaagc
     1201 tatcaaaatg catcccaatc ccggaaggcc tttccggatt gtcggcctgg tagctgatgc
     1261 ccagcaccgg attgcccgaa gtggaaataa gtatggcaat tttgtgatcg aagactactc
     1321 cggtaaaaca gagatagttc ttttcagcga agattacctg cggctcaccc ccgtattgca
     1381 gcaaggcaca acggttttca tcaccggcta tttcaagcaa cggtacaaca gggatgaatt
     1441 cgagttcaaa gtaaacagtg tgtcactggc agaaaccatg aaaaaaaacc tgaccaaaca
     1501 ggtaacgata gannnnnnnn nngtgcatcc tcaggatatt accaaagaca tggtgaactt
     1561 cgttgaaaag aacctacaaa attttccagg taaatcgact ttaaaattta acatcagtga
     1621 gccgaagaac agaatgagaa tcagccttgt gacaatgaac agtggttttg aaatgaatga
     1681 ggaactgata caattcctgg agaaaagctc cgaactcgat gtgcaggtgt taacagtttg
     1741 ataaacaaca ggacttttta gcagtgtcaa tgacaagttt tacacaactt tatgccatag
     1801 ccgcatgtta attatatcgg gcttaaattt gcagtccttc tgagcaaaac cgaaatagta
     1861 atctttaaaa agcaaaactt ataaaatggc aagagaattt acagatgcaa actttcagtc
     1921 ggatgtactg gcttctgata aactgacagt agttgacttt tgggcggaat ggtgtgggcc
     1981 ctgccgggct atcgggccgg ttattgaaga actgagcaaa gagtatgatg gtaaagtgaa
     2041 tgtaggtaaa gtaaatgttg acaataatcc caacatttct atgaactatg gcatcacatc
     2101 tatccccgct atcctgttta ttcaaaatgg ccaggtaatt gacaagttag tgggtgcaca
     2161 gccaaaggcc aactttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaatgatg
     2281 tactgtttcc agttctttgg gattgtgctt atgtttgaaa agcacaagga atagcactgc
     2341 aattacagca gcataggctg caaaggctaa ccagatatta tgccagtcct tatccccgcc
     2401 attcactgta aaatatttat ctatcaaata gccgctaacc ttacttccta aaaaggcccc
     2461 taccccgttc gtcatcatca taaataatcc ctgggcactg gaccgaatgg aagaatctgt
     2521 agttgtttcc acaaacaaag aaccggatat attgaagaaa tcaaatgcca tcccataaac
     2581 aatacaggag agaatgatca tccataagcc gtccgcagga ttaccatagg caaacaggcc
     2641 gaaccgcagc acccaggcta tcatggaaaa caacatcacc ttcttgatac caaattttct
     2701 aagaaagaac ggaatggtca atatgaatac tgtttccgag atctgggaaa ttgacatgat
     2761 gatcgttgaa tattttacca caaatgattc aggaccgtat tccggtactt ttttgaaatc
     2821 gtccaggaaa gtatcaccat acatattggt cagttgcagc gcagcgccta aaaacataga
     2881 aaagataaag aacaaagcca ttttgtattt ggcaaacaac ttaaatgcgn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc
     3001 aaggtaaatg cataaatacc aagtaatata gctgcgacgc ctccaatcaa aaactggttg
     3061 gtatttgcct tgctgtcagt caggttggtt gtccacatcg ctacaataaa tccaattgtt
     3121 ccccacaccc tgataggcgg aaatactttc accacatcaa aggcattctt ttttaaaatg
     3181 gtgtacgaga tcgaattgga aagagagata gtaggcatgt aaaatatcat tgcaatgaaa
     3241 atcacatagt aaagtgtatc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnngcaccat aaagaatatg tagaattcca tataatcttt ctgcattcat
     3361 ccatttatca gcaataatgc cggtaagtgc cggcataaaa agagaagaga gagccagtgt
     3421 tgaaaaaatc gccccgaatt gtgctccgga tcccatattg tttgtgaaaa aataggtagc
     3481 aatagtgatc agccatgctc cccaaacaaa aaattgaaaa aagctgagaa tcgtcaacct
     3541 gattttgata ttcatctgcc tgattttaag tttagacttt taaagattga aagataagga
     3601 tttttaatgc tgccggattg gggttttgcg gcaactgtat ccaccggagc tttgttgttt
     3661 tattgattta acgactatga ttactactac cgatatttct ccagaattaa aagcaattgc
     3721 cgataaggtc agtgcaggcc atcgtatttc tgacgaagat tgtttgtacc tgttcgaaca
     3781 tgccagcctt ggttttgtgg gcagtcttgc caatgcaatc aaggaaagat tacacggtga
     3841 taaagttttc ttcaaccgaa attttcatat cgagcctacc aatgtttgtg tattcagttg
     3901 caacttctgt tcatacagcc ggctgtatgc caaacgggaa gatggnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcacg
     4021 gaagtgcata ttgtgggcgg tgttcatcct aaaatggacc tgcaattctt tgccgacctg
     4081 ctgaaagcca tcaaggctca tcgtcctgac cttcacatca aggcatttac agcagttgaa
     4141 tatgattata tgtttcgcaa agccaaagta agtgtggaag aaggattgaa atttttgatt
     4201 gaagcaggac tggacagcat acccggtggt ggcgctgaaa tctttcaccc ggaaataaga
     4261 gagaaaatat gtgctgacaa agtagatgct gacggctggc tgctcattca tgaaacagcc
     4321 cataaactgg gactaccttc caacgctacc atgctctatg gtcatatcga aaaatttgaa
     4381 caccgcattg accacatgag atggctcagg gaattacagg acagaacaaa gggcttcaat
     4441 accttcatcc cgctgaaatt ccgcaataag gataatgaca tgagtaatgt tccagaaacc
     4501 agtgtggtgg aagacatgaa gatgtatgcg attgcccgaa tctacctgga taattttccg
     4561 cacctgaaag cttattggcc gatgctcggc aggcagaatg cccaactgac tttatctttt
     4621 ggtgtagatg acattgacgg tactattgat gacaccacaa aaatatacag catggcagga
     4681 tcggaagagc aaaaccctgc tatgtcaaca acagagctgg taaatcttat taaacaggta
     4741 aaaagggtgc cggtagaaag agatacggtg tacaacgaga taaaaaatta ttcaggcact
     4801 gttaacgaac caaacccggc attgaattga ccaggctatc tcttttattg atctttattc
     4861 ttttgaatat agcaggcaaa agttttgccc agcagaaata cactattact tacgaaaatt
     4921 tctcctttta tatttttgac agtactggta atccggtgaa acctgcatgg gacaagaaaa
     4981 tcaccacgca aattgtcatc aacgaccagc aatcctatta ctacaccgta tttaatggaa
     5041 acaaaccgat tgaacccggc aaagtacttg gaaaaaattt tttacctcat accaattatg
     5101 tacatctgca aaacaaaatc ctggtatccc aatttggtga taaatatagg gtaagagagc
     5161 ctataatcga ttttaaatgg caattactta cggggaagaa gatagttgcg ggatttgaat
     5221 gtaaaatggc ctattgtatt taccagggtg attctttggt agcaagctat tcagaaaaat
     5281 tgcctgtgct atttggtccc cttacttata ccggtttacc tggcaccata ttagaaacgg
     5341 atcattatcg taaagagaac gtttaccgga caactgcatt aaaagtggaa aacactgcaa
     5401 acccgattgt agaacccaaa gatggcaaac taatctcctg ggagcaatgg aaaaagatta
     5461 ttgccaacag aagatctgat aatggagtaa taagagtagt cagatggtga tttgactact
     5521 cttattttac gtctttaaaa actacattac cggcccttcc tcatcgtttt cttcctctgc
     5581 atctggtaag tcatcctccg caaatccatt atcctcttcc ggttcttctt cgaaggtcag
     5641 aatggtttct gttacttcca aagtgccatc tggtgttaca aatgtttcag tgatttcttc
     5701 taccagaaca ggtgtttctt ccattacagg tggcggcact gctaccggtg ccggcttaac
     5761 ttttttaacc ggcgcctttt tcttaggagc cggcttttta gccaccactt ttttctttgc
     5821 aacttttttc accgcttttt tcttcactac tttggctttc tttttggcaa caggttttgc
     5881 agggcggcga acaaccttct tagccgcttt ttttgatttg gcttttgcct ttactggttt
     5941 ctttgctgct ttcttcttaa caacctttac cttttttttg gcaaccgctt ttttcttcgc
     6001 tgctggtttt gccaatttct ttgctttctt tttagtagcc atggctgata gttttttata
     6061 tgttgaatat gcgggatagt aagcggataa taaaattaca tattcatagt aatcaaatca
     6121 tgcaatggtc aaaatttaaa cagccttaac ttagcaacaa cggttctttt acctttgctg
     6181 ccatggcaga agatttacag ataatacggg gtagtaaaac agtccaatat gaatccctgg
     6241 taccgcaaat aaaaggtttg ctcgaaggag aaaccgacct ggtggccaac ctggccaatg
     6301 ttgccgctgc actgaaagaa cagtttggct ggttttgggt cggattttat ctcgtaaaag
     6361 acaatgaact ggtactcggc cccttccagg gaccggtcgc ctgtacccgt atccgcaaag
     6421 gccggggtgt ttgtggcagc agttgggaac agcaaaaaac cctgatcgtt ccggatgttg
     6481 aaaagttccc cggacatatt gcctgcagca gcctttctaa atctgagatc gttgttccgg
     6541 ttatccgcaa taatgaagta ctgggtgtgc tggatgtgga cagcgaccaa tacgatcagt
     6601 ttgacagcac tgatcagcaa tacctggaac agatcgtcag cctgattcaa ttctaaccaa
     6661 tgctgaaaac cctttccatc atcgtaacgg gcaaagtgca aggtgtttgg taccgccaat
     6721 ccgccaaaga aaaagcaacc gaactgggtg tcaccggtaa tgtccgtaat caacccgatg
     6781 gttctgttgc tattattgcc actggtttgg caaagcagct ggatcaattc attgagtggt
     6841 gccgccaggg acctccccgt gcccttgtaa caaatgtgga agtnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcgaacgata acacctgtca atgggtgaca
     6961 tgcaggtaac tcacgaagag tatagccgca tggcaaataa tcagcagcaa tttgctaaag
     7021 aacacacatt tgctgtaatg ccttcttacc tttttcagtc tgtttttatg gaaatgatga
     7081 atatgggcaa gctcatccca ggaaggcaca tgactatgcg ccttttctcc gccaggatgt
     7141 tgtttggctc ccatcggtaa atgaccggta ttggcagctt taccagggtc atcgctatga
     7201 tggttagtag caatatgccc ggactgctgt tgtgtacccg ccagggtagt gattggtttt
     7261 gccatcagta aggcaaataa cattgtggtg aagatgaaat atcgtgtcat aacatttggt
     7321 tttatgaccc aaattttcct tcaccgatgg ggcgaaatca aggtaggaaa tgcggaaaaa
     7381 ataaatgccg tagttcaacg gggaaaagga ctttgcaact gcgtatgaat gctcattcaa
     7441 aacaggtacg tagtagtacg caagaacaat atgttactac gcattagggc ttctttaact
     7501 tgcaaattcg caaaactgaa cttatattag gagaacattc ctgccctctt taagctccct
     7561 gnnnnnnnnn naattatgaa tttcttgaaa tctaaaacat ttctcctatt gcttttacta
     7621 ttagcaatca cagcaatagt ttatctatta ttaaggccaa aacctggctt aacaatagaa
     7681 gaaccaagac cccagatgaa tattaataat tgctttacaa taaccagaga tgacatggtt
     7741 aactcatggt taggtcctaa ctggaatgtc aaaggagatc ccaactatat ttccggtatc
     7801 atgtttgaag caattgatac tgaccccaat aacattaaaa tagttgccta cccggttgat
     7861 aactcatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatagttta aaagcgcaaa
     7981 aaacccgtta tgactttgca tctagtgaca ttgatccatt tggagacctt attcacttca
     8041 attttatgag acttattcca aaaccagatg ctacaaaccc tgctaatctt agctttgttg
     8101 ttgaatatgt aaaaattaaa ggaggaagtg aaactgtttt gtcaagaggt gataccaaac
     8161 catgtcctcc ttattgtcca actgatcctg acttgattaa ataaagtgaa tcccatcgtt
     8221 gaatcaataa tgacatggtc agaggtctgg gcattgctca tacctctgat cttttttttg
     8281 atttataaac aaaaagataa taccacaaga cctataattt actatatatt catcggtctt
     8341 attcttaact tattagccac tctggtaata gtattctttt ttannnnnnn nnnnnnnnnn
     8401 nnnttgagaa acaatactat cttttataac cttcattcaa ttgcaagagt atcattcttt
     8461 agttggtaca ttattacaat aagaaaatat tatcgccccc agctatataa gattttgctg
     8521 gctgcatatt ttgtattcct ggcattcaac tttattttca atgactcaat tactgtatat
     8581 aacaatgatt tagcaactgc tgaaacaatt acgctacttg tattatgcat cttctttttt
     8641 cttcgctcaa ttcaagacga tagtgaagta aactggttga aacatccggc ctttcttttt
     8701 tgtgcgggta tatttctata tgaatcagtt agctttttca ttaacctctt tttctataaa
     8761 atggcagaaa aaaacttgtc ttttggatct ttgatgctcg atattcaaaa cctgattttc
     8821 cttattcttt gtttaatgat ttcaatgggt ctttatagta atcgaactaa aaatgagtga
     8881 tgtatcaact atagtcatag tgggaattgc ggtcatgctg ctcatggtgg tgagcatctt
     8941 gctggctgtt atttacaacc agcgtaaaaa agatcagcac aggaaggccg tagaacaact
     9001 ccgtgaacaa cagcaaaacc agctcatcga agcggcggta cgcagcg
//
LOCUS       DAORLS010000114         8960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4204_length_8960_cov_2.029197, whole genome shotgun sequence.
ACCESSION   DAORLS010000114 DAORLS010000000
VERSION     DAORLS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4204_length_8960_cov_2.029197"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PK977_09850"
     CDS             <1..686
                     /locus_tag="PK977_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18463.1"
                     /translation="VIFAPVYPYCMRYKTILFLLIVFANTLSAQNEMLVTAGKNCGKL
                     KIGMTEKQVRDLLGNDLLVKTYKEEMTAFWYHNRTLRMDSITQFVLGFDKCLELSDSL
                     NKQWPVFKLFLLNDRVNCMIVTSYLDDSLLVKQVLVNKKIRFYDSRDKCVKLFGNTYI
                     NTPYPGYEEYVYYKNGIQLLFDEGILKTFKIFKPDPNFLRRIAARSEIIKSEFMAIDK
                     EKGGIDWPD"
     gene            734..2092
                     /locus_tag="PK977_09855"
     CDS             734..2092
                     /locus_tag="PK977_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRF18464.1"
                     /translation="MDIKKNILETIGNTPLIMLNKVTKDVPCPVLAKVDYFNPGNSIK
                     DRMALKMVKVAEQEGKLKPGGTIIEGTSGNTGMGLALAAVVKGYKCIFVTTDKQSKEK
                     ADILKAVGAEVIVCPTNVMPEDPRSYYSVAARLAKEIPNSYHMNQYDNLANRLAHFET
                     TGPEIWNQTDGKITHLVCTAGTGGTITDTAMYLKEKNPNIQVWAIDVYGSLLTKYFRT
                     GEIDMNEVHPYISEGFGEDFVPKNYDMSVIDHFEQVTDKDGAVMARKLAKEEGLFCGY
                     SAGSCIQGLLQLKDRLKKDDLVVAIFHDHGSRYVGKIYNDQWMIERGFIDVKTFRDVV
                     SARGVKKLITVEPAQTVAEAVELMKKYDIEHIPVMNGTGPVGAISEGGLFQKIFDNPE
                     LKHAKVEEVLEPAFPIVAFDTPVEKLSHLINKENGAVLAKDEAGSFHIITKYDVIQSL
                     GK"
     gene            2533..3318
                     /locus_tag="PK977_09860"
     CDS             2533..3318
                     /locus_tag="PK977_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRF18465.1"
                     /translation="MLSFIDQTNQKVVLPDTPNRIISLVPSQTELLFDLGLDKEVVGI
                     TKFCIHPEIWFRSKPRVGGTKQVKMEIIHQLEPDLIIANKEENVKAQIEELAGKYPVW
                     TSDIHNLEDALVMIRDIGLITGKQAMANRIAEQIAEGFTTLTVPGHRLRTAYLIWKDP
                     YMTVGNDTFIHCMLETAGFDNVFSERKRYPAINIDDLKKSGCELLLLSSEPYPFQEKH
                     MDELQQQLPDTRILLADGEMFSWYGSRLLKAPAYFTSLRNQLK"
     gene            3376..4491
                     /locus_tag="PK977_09865"
     CDS             3376..4491
                     /locus_tag="PK977_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18466.1"
                     /translation="MRFIDNQNGRETKEGDKPSRKKPMYPVNEKLRSYLKNRGREVKL
                     PVTYNNLLNFTWSTPLKDKHGKDTYWEKTSYDSREWDFIREGLVKIYAILKTEGDFSY
                     TSHLDVARVDYCAFGNSNPFRIRIVNKFNDNYDHYYIKIADASRIYGLELEHILSPNR
                     ITFYTCDNTLVEEHIPGIPGDVFVQQMMEAPETNKIRLAKEFVKFNERCFVRLLGDMR
                     SYNFVIGITPDIEDYQYRIRCIDFDQQSYEGRKSLYLPQFFKENYPFVDLCLSHLNRE
                     SIEQYQAEERTMITFRLVSSRYRIKDLLDIMTQDQISTTEKVNQLKKELCEYMHTNSY
                     EKCQSMGQIVKTHLKETLRKNLLLIQKNLGKTQRSLL"
     gene            4594..5661
                     /gene="fbaA"
                     /locus_tag="PK977_09870"
     CDS             4594..5661
                     /gene="fbaA"
                     /locus_tag="PK977_09870"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRF18467.1"
                     /translation="MKKYRAGVLFGDELEALYNDAKENQFALPAVNTIGTNTINATLE
                     TAAKVNSPVIIQFSNGGAQFIAGKGMPNDNLQGNIAGGISGALHIHNVAKYYGVPVVL
                     HTDHAAKKWLPWVSGLIDAGEQFYKEKGQPLFSSHMLDLSEEPIEENIHTSVEFYKRM
                     SPLGMSIEIELGVTGGEEDGVDNSDVENSKLYTQPEDVAYAYRELSKVGKLFTVAAAF
                     GNVHGVYKPGNVELRPVILHNSQVHIQKEFNTGEKPVYFVFHGGSGSPQHQIREAIGY
                     GAIKMNIDTDLQWAFWEGVLNNYKKHEGYLQGQLGNPEGEDKPNKKYYEPRVWLRKGE
                     ETFIQRLEVAFEDLNCINRNS"
     gene            5892..6767
                     /locus_tag="PK977_09875"
     CDS             5892..6767
                     /locus_tag="PK977_09875"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRF18468.1"
                     /translation="MPAKKIAELLGDKAEFLLNHQSKTISKDQLHLPGPDFVDRIFAA
                     SDRNPQVLRNLQAMYGTGRLANSGYLSILPVDQGIEHSGGASFAKNPIYFDPENIIKL
                     AIEGGCNAVATTFGGLGFLSRKYAHKIPFIVKINHNEFLTYPNKFDQVMFASIKASWN
                     LGAAAVGATIYFGSDEATRQIQEVSKAFEMAHELGMATILWCYLRNPAFKTKEKDYHV
                     SADLTGQANHLGVTIEADIIKQKLPENNGGYLALNTKESAYGKNDKRIYEELTTDHPI
                     DLCRYQVANCYMVPV"
     gene            6764..6961
                     /locus_tag="PK977_09880"
     CDS             6764..6961
                     /locus_tag="PK977_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18469.1"
                     /translation="MINSGGASSGASDMAEAVTTAVVNKRAGGMGLISGRKAFQRPMK
                     EGVEILNAIQDVYLDKEVTIA"
     assembly_gap    7357..7370
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7371..>7658
                     /locus_tag="PK977_09885"
     CDS             <7371..>7658
                     /locus_tag="PK977_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18470.1"
                     /translation="LIDSEPLWTEAGVETLQHYGKSLTQEQYHSSTGLRTEEWIEHWF
                     RHFEIDMSHAPAAIDVIIQKAIDKIGIHGQPLPGIDNIFRFFKTKDYRMGLA"
     assembly_gap    7659..7676
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7677..7989
                     /locus_tag="PK977_09890"
     CDS             <7677..7989
                     /locus_tag="PK977_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRF18471.1"
                     /translation="LVEVVVKKLGIENVFDTVASAGSLPYGKPHPQVYLDCAAQLGCP
                     AVQCIAFEDSFNGMIAAKAARMKCVVVPAAADFDHPKWNAADLKLKSLAEFDEGKLTE
                     L"
     gene            complement(8047..>8677)
                     /locus_tag="PK977_09895"
     CDS             complement(8047..>8677)
                     /locus_tag="PK977_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRF18472.1"
                     /translation="IFHNRQLSEPSLTNSFVFVVNFLTFYFLKPTNMYKKLALIFVAA
                     GSLFFLACNSGGGGMSATAKKNKEVNDAIMKAYEAGDFSKMGDYIAADAVDHGGEKGD
                     VKGLDNIVSEMKRYREMMPDMKSTVIKEMVDDEYVFYWASVSGTMQGKAMTMTSLDVT
                     KFKDGKAVEHWVFMDPKEMMAMMQESGAGGTEQTAPADSTGVQPQPKTE"
     assembly_gap    8678..8750
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8751..>8960
                     /locus_tag="PK977_09900"
     CDS             <8751..>8960
                     /locus_tag="PK977_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18473.1"
                     /translation="LAGLLLIVFIVLTSGDYKGRYDGGYKDMVDELYSLAVKQNENLE
                     TIEDDIEKFYKKKSEALEKYNSYTA"
BASE COUNT         2723 a         1864 c         1924 g         2344 t
ORIGIN      
        1 gggttatctt cgcccctgtt tacccctatt gtatgcgata taaaaccatt cttttccttc
       61 tgattgtttt tgctaatacc ctgtcagcac aaaacgagat gctggttaca gccggaaaaa
      121 attgcggaaa actaaaaatc ggcatgaccg aaaaacaagt ccgtgacttg ttgggcaatg
      181 acttgctggt aaaaacatac aaagaagaaa tgactgcctt ctggtatcac aaccgcactc
      241 tccgtatgga tagtatcaca cagttcgtat tagggtttga caaatgcctt gaattaagtg
      301 acagcctcaa taaacaatgg ccggtgttta aactgttttt actaaatgat agagtgaatt
      361 gcatgattgt aacctcttat ctcgatgaca gcctattggt aaagcaggtg ctcgttaata
      421 aaaaaattcg tttttatgat agccgggaca aatgtgtgaa gttatttggc aacacttata
      481 ttaatacgcc ttaccctgga tatgaagagt atgtatatta taaaaatgga atacagttat
      541 tatttgatga ggggatattg aaaacattca aaatctttaa acctgatcct aatttcctca
      601 ggcgaattgc cgccaggtca gagattataa aatctgaatt catggccatt gataaagaaa
      661 agggcggcat agactggcct gactgaaaaa tcaagataac tgtattttgt acctttgctt
      721 ttcaaataaa aatatggata taaagaaaaa tatactggag acaattggta acactcccct
      781 gattatgctg aataaagtta ccaaagatgt accctgcccg gtgctggcta aagtggatta
      841 tttcaacccc ggcaactcta tcaaagaccg catggcgctg aagatggtaa aagtagcaga
      901 acaggaaggt aaattaaagc ccggaggcac catcattgaa ggaaccagtg gcaatactgg
      961 tatgggtttg gcgctggctg ccgttgtaaa aggttataaa tgcatcttcg ttaccacgga
     1021 taaacaatcg aaagaaaaag cagatatact aaaagctgtt ggcgctgagg taatagtctg
     1081 tccaacgaat gtgatgcctg aagatccaag aagctattac agtgtagcag caagactggc
     1141 caaagagata cccaactcct atcatatgaa ccagtatgat aacctggcaa accgcctggc
     1201 tcattttgaa accaccggcc cagaaatttg gaatcaaacc gatggtaaaa tcacccatct
     1261 tgtttgcaca gcaggtacgg gtggaactat aacggatacg gctatgtacc tgaaagaaaa
     1321 gaatcccaac atacaggtat gggctattga tgtgtatgga tcacttctta caaaatattt
     1381 ccgtaccggt gagattgata tgaatgaagt gcatccttac atcagcgagg ggtttggtga
     1441 agattttgta ccgaagaatt atgacatgag tgtaattgat cactttgagc aggtaacgga
     1501 taaggacggc gctgtgatgg caagaaaact ggcaaaagag gaaggtttgt tctgtggtta
     1561 cagtgcggga agttgtatac agggattatt gcaattaaaa gaccgtttga aaaaagatga
     1621 tcttgtggtt gctatcttcc atgatcatgg cagccggtat gtaggaaaaa tttacaacga
     1681 tcaatggatg attgaaagag gatttatcga tgtcaaaacc tttcgtgatg tagtaagtgc
     1741 aagaggagtt aaaaaattga tcactgttga gcctgcacaa actgtagcgg aagccgttga
     1801 actgatgaag aaatatgata tcgaacatat tcctgtcatg aatggtacag gcccggttgg
     1861 tgccataagt gaaggtggtt tattccagaa aatttttgat aaccctgagt taaaacatgc
     1921 taaagtggaa gaagtgctgg aaccagcttt cccaatcgta gcatttgata cacctgttga
     1981 aaaactcagc caccttatta ataaagaaaa tggtgcagtg ctggcaaaag atgaagcagg
     2041 cagtttccac attattacta aatacgatgt tatacaatcg ttgggcaaat aaaaaccaac
     2101 atttgtaaat cactgaaagc cattgtaaaa taagcaatgg cttttttgtt tttcgtaata
     2161 ttacgattag atacaccggt attgccgcaa aaaagaacag tcagtttact attaaaaatc
     2221 gggcagtttt gctcttgtgt aactatgata gcaggtatat ttttgtgtca gattaatcca
     2281 atatccaatt cagaaaaaac cgaaaccaat tccttagaaa aacccgaaaa tccaacgtcc
     2341 agatcgaaaa accgatcgat ccaagatcct tgaagaacga cccaaagtgt caccgtgtcc
     2401 aaatccaatg aaagaaattc aacgtccgga accctcctgc taaatcacat ttagcaggag
     2461 gcgcggtgaa cctcaattct cctctccttt tctcagactt ttttgattca gtactttgca
     2521 gcaaattgtt gcatgttgtc gtttattgac caaaccaacc agaaagtagt ccttccggac
     2581 acgccaaacc ggataatttc tctcgttcct tcacaaacag aattactatt tgatctcggg
     2641 ctggataaag aagttgtcgg catcactaaa ttctgcattc acccggagat atggtttcga
     2701 agcaaaccaa gggttggcgg caccaagcag gtgaagatgg aaatcattca ccaactagag
     2761 ccagacttga ttatagccaa caaagaagaa aatgtaaagg cacagatcga agaactggcc
     2821 ggcaaatatc ccgtctggac aagtgatatc cacaaccttg aagatgccct ggtgatgatt
     2881 cgggatattg gccttattac cgggaaacag gcaatggcaa atagaattgc agaacagata
     2941 gcagaaggct ttacaacatt gacagttccc ggccaccgtt taagaacagc ttatcttatc
     3001 tggaaagatc cctacatgac ggttggcaac gacacattca ttcattgtat gctggagact
     3061 gctggttttg acaatgtatt cagcgaaaga aaaagatatc ctgcaatcaa catagatgac
     3121 ttaaaaaaat caggctgtga attactgctg ctctcttcag aaccctatcc cttccaagaa
     3181 aagcatatgg atgagttgca gcaacaatta cccgatacca ggattcttct ggcagatggt
     3241 gaaatgttca gctggtatgg cagccgtctt ttaaaagctc ccgcctattt tacttcacta
     3301 cggaatcagt tgaaataata cgaacttagc atggctcaga ttcactattg acttttatta
     3361 tacactattc acctaatgag atttatcgat aaccagaatg gcagggaaac aaaggaaggt
     3421 gataaacctt cccgcaagaa acctatgtat cccgtaaacg aaaaacttcg gtcttacctg
     3481 aaaaaccgtg gtcgtgaagt gaaactgcct gtcacctata acaacctgct caactttacc
     3541 tggtccacac cgttgaaaga caagcacggg aaagacactt attgggaaaa aacttcctac
     3601 gatagccgtg agtgggattt tatcagggaa gggctggtaa aaatttatgc cattttaaaa
     3661 acagagggag actttagtta caccagtcac ctggatgtgg caagggttga ttactgtgcc
     3721 ttcggcaact ccaacccttt ccgtatacgc attgtcaata agttcaacga caactacgac
     3781 cattattata ttaagattgc cgatgcttca aggatatatg gattggaact ggagcatata
     3841 ctctctccca atcgcatcac tttttacacc tgtgataata ccctggtgga agagcacatt
     3901 cctggtattc ccggtgatgt atttgtgcag caaatgatgg aagcaccgga aaccaataag
     3961 atcaggctgg ccaaagagtt tgtgaagttt aatgaacgat gctttgtacg gttactgggc
     4021 gatatgcgca gctacaactt tgtgataggg atcacccctg acattgaaga ttaccaatac
     4081 cgcatacgct gcatcgattt tgaccagcaa tcatacgaag gaagaaaaag cctttacctg
     4141 ccacagttct tcaaagagaa ttatcctttt gttgatcttt gcctttcaca cctcaacaga
     4201 gaatctattg aacaatacca ggctgaagaa agaaccatga tcactttccg gctggtaagt
     4261 tcccgctatc gcatcaagga tttactggat attatgacgc aggatcaaat ttcaacaacg
     4321 gaaaaagtaa atcagctgaa aaaagaatta tgcgaataca tgcataccaa ctcctatgaa
     4381 aaatgccagt caatgggaca aattgtgaaa acacacctga aagaaacgct tcgcaaaaac
     4441 ctgctgctga tacaaaagaa tctgggtaaa acacaacgtt cacttttgtg agtaaactca
     4501 atccatctaa ttcaataaaa agtctatatt agcagcccta tagcgattgc ctgcctgccg
     4561 aaaagtattt tctactttta aaattacaaa ctcatgaaaa agtatagagc cggtgtactg
     4621 ttcggtgacg aactggaagc actctacaac gatgccaaag aaaaccagtt tgccctgcca
     4681 gcggttaata caattggcac caacaccatt aacgctacgc tggaaacagc tgccaaagtc
     4741 aactctcccg ttattattca gttctcaaat ggtggcgcac agtttattgc cggtaaagga
     4801 atgcctaatg ataacctgca gggtaatatt gccggcggta tctccggcgc cttgcatatt
     4861 cacaacgtgg caaagtacta tggagtgccg gtggtattgc atacagacca tgccgccaag
     4921 aagtggttac cctgggtaag cggattgatt gatgccggcg aacagtttta taaagaaaaa
     4981 ggccagcctc ttttcagttc gcatatgctt gacctgagtg aagagccgat tgaggaaaac
     5041 attcacacct cagttgaatt ttataaaaga atgtctcccc tgggtatgag catcgagatt
     5101 gaattgggtg ttaccggtgg tgaagaagat ggagtggata acagcgatgt ggaaaattcc
     5161 aagctctata cacagccgga ggatgtagct tatgcctaca gggaattaag caaggttgga
     5221 aagctgttca ccgtggcagc ggcatttggt aatgtgcatg gtgtgtataa accgggtaat
     5281 gtagaactgc gtcctgttat tctgcacaac agccaggtgc atattcaaaa ggaatttaac
     5341 accggggaaa agcctgttta ttttgtattt catggaggca gcggctcacc ccagcaccag
     5401 atccgggaag caatcggcta cggcgctatc aaaatgaata ttgacaccga cctgcaatgg
     5461 gctttctggg aaggtgtgct gaacaattat aagaaacatg aaggttacct gcagggccag
     5521 cttggcaatc cagaaggtga agacaagccg aataaaaaat actatgaacc aagggtatgg
     5581 ctgcgcaaag gcgaagagac tttcatccag cgccttgaag tggcttttga agatttgaat
     5641 tgtatcaacc ggaacagtta agttttttac cacaggttac tcagatacac ttttacaaat
     5701 tacacagtat ttttctgcgt aacaatctgc gtaatctgtg gtcaattttc tcatctctac
     5761 tgtcgactcc agactaacga ctattgacgc aagactccca actttccccc cgcctcccta
     5821 cctttgccga acatctgtta gtatactttt attaacaaat caacctgtaa tcttataaac
     5881 ccaacattgc tatgcctgct aaaaaaattg ctgaactcct cggtgataag gccgagtttt
     5941 tactcaatca ccagtcgaaa accatttcaa aagatcagct gcacctgccc gggcctgatt
     6001 ttgtagatcg catcttcgct gccagtgacc gtaacccgca agtactgcgt aacctgcagg
     6061 ccatgtatgg taccggccga cttgccaaca gtggttatct ttccatacta cctgttgacc
     6121 agggtattga acacagcggt ggtgcttctt ttgccaagaa cccgatttac ttcgatccag
     6181 agaacattat caaactcgct attgaaggtg gctgcaatgc agtggctaca acatttggag
     6241 gccttggctt tttgtcccgc aagtatgcac ataagattcc tttcatcgtt aagatcaatc
     6301 ataacgaatt cctcacttac cccaataagt tcgaccaggt aatgtttgct tccattaaag
     6361 ccagctggaa ccttggcgct gctgcagtgg gcgccactat ttattttggc agtgatgaag
     6421 ccacccgcca gatacaggaa gtaagtaagg cctttgaaat ggctcatgaa ttaggtatgg
     6481 ccaccattct ctggtgctac ctgcgcaacc cggctttcaa aacaaaggaa aaagattacc
     6541 atgtatcggc tgatcttacc ggccaggcca atcatctcgg tgttaccatc gaagcggata
     6601 ttatcaaaca aaaactgcct gaaaacaatg gcggttacct cgctttgaat accaaagaat
     6661 ctgcctatgg taagaatgat aaacggattt atgaagaact cactaccgat cacccgatcg
     6721 atctttgccg ctaccaggtg gccaactgct acatggtgcc ggtttgatca attcaggtgg
     6781 tgcttcatcc ggtgccagtg acatggcaga agcagtaacg actgccgtag tgaataaacg
     6841 agccggtggt atgggactga tcagcggccg taaagctttc cagcgaccca tgaaagaagg
     6901 tgtagaaata ttgaatgcca tacaggatgt ttatctcgat aaagaagtga cgatcgcata
     6961 aataaaaaag ccgggatcaa ttaatagagc caatcagaac cgattctgtt tttgataaat
     7021 tttgaaacgg cttttatgtg tttgaacata aagaaaactg acaccttgct taaaagagta
     7081 tttgaacaaa acaatatcta cacccggcat tttgccattg atacagatac cttcataggc
     7141 ccctatgaaa agtcggatgt ttctgtaaaa tagattacac tttgtatgaa tggtgccggt
     7201 acatcgttgt gcctggctat actgttatca caagagctga ctgggttacc acttgagacg
     7261 aagctgggta tttcgttcaa aaacaaattt ggaagaatgc ccttcctgtt gaaagtttgc
     7321 agcatgcagt ataaagcagt gatttttgat atggacnnnn nnnnnnnnnn ctgatcgaca
     7381 gcgaacccct atggacagaa gcaggtgtgg aaaccctgca gcattatgga aaaagcctga
     7441 cacaggagca atatcacagc agcacagggc tgcgaactga agaatggatc gaacattggt
     7501 tccgccattt tgaaatagat atgtcccatg cacccgccgc catcgatgtt attattcaaa
     7561 aagcaattga taagataggt atacacggac aaccgctgcc gggtattgat aatatattcc
     7621 ggtttttcaa aacaaaagat taccggatgg ggctggccnn nnnnnnnnnn nnnnnnactg
     7681 gtggaggtgg tggtaaaaaa gttgggtatt gaaaatgtat ttgatactgt agcttctgca
     7741 ggcagcctgc cctacggcaa accgcacccg caggtgtacc tggattgtgc ggcacaattg
     7801 ggttgccctg ctgtgcagtg tattgccttt gaagattctt ttaatggcat gatagcggcc
     7861 aaagccgccc gtatgaaatg cgtagtggtg cctgctgcgg cagactttga tcacccaaaa
     7921 tggaatgctg ctgatttgaa attgaaaagc ctcgctgaat ttgatgaagg gaaactcacc
     7981 gaattatgaa tgaaaatgcc ggctcatcag agccggcatt tttcaaacag ttttaataaa
     8041 gctctattac tccgttttag gctgaggctg tacacctgtt gagtccgcag gggcggtttg
     8101 ctctgtgccg cctgcaccag attcctgcat catggccatc atttctttag ggtccataaa
     8161 tacccaatgc tctacggctt tgccatcttt gaatttggta acatcaaggc tggtcatcgt
     8221 catagccttt ccctgcatgg tgccggatac acttgcccag tagaaaacat attcatcatc
     8281 caccatttct ttaatgacgg ttgatttcat gtcgggcatc atctcccggt agcgcttcat
     8341 ttcgctcact atattatcca gtccttttac atcccctttc tcaccgccgt ggtctacagc
     8401 atcggcagca atatagtcgc ccatcttgct gaagtcacct gcctcgtagg ctttcatgat
     8461 ggcatcattt acttctttgt tttttttagc tgtggccgac ataccaccac caccgctgtt
     8521 gcaggcaagg aagaacaggc ttccagctgc aacgaaaata agtgcaagtt ttttgtacat
     8581 attggttggt tttagaaaat aaaaggtaag aaaatttact acgaagacaa aagaatttgt
     8641 taaggatggt tcgctcagct gccggttatg gaatatcnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccttgcgggc
     8761 ctgttgctga ttgtttttat tgtacttacc tccggcgatt ataaaggccg ctatgatggt
     8821 gggtataaag acatggtaga tgaactctac agcctggcgg taaaacaaaa tgagaacctg
     8881 gaaactattg aagatgatat tgaaaagttt tacaagaaaa aatcggaagc ccttgagaaa
     8941 tacaacagct acacggctta
//
LOCUS       DAORLS010000115         8926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4226_length_8926_cov_2.167174, whole genome shotgun sequence.
ACCESSION   DAORLS010000115 DAORLS010000000
VERSION     DAORLS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_8926_cov_2.167174"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="PK977_09905"
     CDS             <1..1891
                     /locus_tag="PK977_09905"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRF18474.1"
                     /translation="KYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGM
                     IAIGVGGADACDVMAGLSWELLMPKLIGVKLTGKLNGWTAPKDVILKVAGILTVKGGT
                     NAIVEYFGEGATSMSCTGKGTICNMGAEIGATTSTFGYDESMSRYLKATGRAEVAEAA
                     DKIKEYLTGDAEAYANPEKYFDQVIEINLSELEPHLNGPFTPDLATPVSQMKEAAAKN
                     GWPTKVEVGLIGSCTNSSYEDISRAVSLAKQVKEKGLKLKSEFTITPGSEQVRYTIER
                     DGYLDTFANIGATVFANACGPCIGMWDRMGAEKQEKNTIVHSFNRNFAKRADGNPNTM
                     AFVASPELVTALAIAGDLTFNPMTDTLTNDKGEQVKLDPPSGDELPVKGFAVEDAGYQ
                     APAEDGSRVVVDVKPDSKRLQLLYPFAAWEGTDIKGLKLLIKAKGKCTTDHISMAGPW
                     LKFRGHLDNISNNLLIGATNFFNEKTDSVKNQLTGEYGTVPNTQRAYKAAGIGTIVVG
                     DENYGEGSSREHAAMEPRHLGVRVVLVKSFARIHETNLKKQGMLAITFADKEDYNKIQ
                     EDDTIDVIGLTSFQPGKQLQLVLNHNDGSSETINVNHSYNEQQIEWFKAGGALNVIRA
                     EFAKG"
     gene            complement(1944..>2274)
                     /locus_tag="PK977_09910"
     CDS             complement(1944..>2274)
                     /locus_tag="PK977_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18475.1"
                     /translation="GNQLNSPQSIQLSDASANDEITIKYHHCGRVGKKRVLTIKDGQE
                     KVLKEIRFADVNNPVSGMSCKVKDIISLKKANNTVLKLYYTSSELPNGRLLASIITGS
                     KTSAGRP"
     assembly_gap    2275..2284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2677..2734
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2735..4007)
                     /locus_tag="PK977_09915"
     CDS             complement(<2735..4007)
                     /locus_tag="PK977_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18476.1"
                     /translation="MKKIILIIAMVALGGSINNVKADPCYFGNIGIDVKTTYSDQLGR
                     CVIVFDFYFDIQHNPGGKYFWVHIWPTSLYTGNFSYDKPPTFSTSGNGNGYLINSLTT
                     FGFFHQGSALEPQLTYLPDPTTPGFQSSYTITETVGTPDRYTIRGLTITLPQGCSIPQ
                     SLTADAWESQADQSQKVGCSSTNIPFYVNDPGLRGFLFCQTPRTYAFEVYTISNTDMN
                     INYKVAIDNGDGIYNAISDNIIVNSGTTLLDATNSYKYQSGIQGYLPYSNQKPEADRS
                     LWVVVTTPSRPNEFYALITNSCTPLPVQFASFTAWRNKENVMLKWVTATELNNKGFYI
                     ERKSGNGEWQVMGYIASQAANGNSNENLFYNYTDFNNLKAVSQYRIRQVDLDNKASYS
                     DIRLVKGLEQTGNVVVFPNPSPDGQLNIVLDN"
     gene            4320..4745
                     /locus_tag="PK977_09920"
     CDS             4320..4745
                     /locus_tag="PK977_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18477.1"
                     /translation="MQRLKPGVDIVLDILNAVAEAQPHSEFAQSILRQYQERGGLSKK
                     QLQGLYGKAQKLQSIPPGKLATLEAIILKKHTKERSGIPATSPLYQKDSITGQLIDSI
                     LKQFPSHKRVLYLKSKYDNNEVLSSVELSELNRFSKLLK"
     gene            4799..5404
                     /locus_tag="PK977_09925"
     CDS             4799..5404
                     /locus_tag="PK977_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF18478.1"
                     /translation="MTKQYTQLLSAEELTFHVEACSRNSRESQKIIYGSFYGYAMAIC
                     DRYTNKQEDAVEILNDGFLKVFKDIHNFKPAYADLISSFKGWLRKIMIYTAIDHFRKH
                     HKHQLVSKLDDVTYQLPPVYDDAVDKLSYEEIIRAVQELSPAYRAVFNLFVIEGFSHE
                     EIAEHLGISTGTSKSNLFKARLQLQKILFKNYHIQVAKNAV"
     gene            5394..6800
                     /locus_tag="PK977_09930"
     CDS             5394..6800
                     /locus_tag="PK977_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18479.1"
                     /translation="MQSEEFDKKVREAASHHHPAYDEQAWDKMHKLLDKHLPEEKDDR
                     RRIIFYLLFFLLLGGGIWLLLGKLGEQPQKPISTQARQKETHTSSGGNNVTGEIEGRN
                     NQPRQTTTVSAKEVTIAGTPANTTKTTSQNQHSNFLSNKKMNNPVPVTDKIIAVLSGK
                     ESNKNINAGKEVQKNSDKDELSPDQQQKVKEELANRKESILPGVITNKKTENQPSANE
                     KADTTILNKSIVQDSVAVNSVNKTAKKQADKKDKDKKTKNKKSNFFFVSLSAGPDVSY
                     TTGGQLGTTKLTGGIGLGYTYKNRISVRTGFFSGRKVYAASPDAYNPPAIFWNYYPYL
                     EKVDANCKVYEIPVLASYHFGKPGKQHWFVSAGLSSLIMKQEDYHYAYKYTPNGATLY
                     RDWTVKNENKHFFSVATVSGGYQKSIGKSITFTAEPYLKLPLGGVGYGRVKLNSTGIL
                     FSVSVKPFQQNKQTDKKP"
     gene            6857..8200
                     /locus_tag="PK977_09935"
     CDS             6857..8200
                     /locus_tag="PK977_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF18480.1"
                     /translation="MKKHFILLQLLFVIASGILFNSSCSKGGGNTPPPANPCTGVTVV
                     VNGTVANPTAPGASDGSIIVTATGGSGFTFSLNNGAFQSSATFSGLAAGAYTVTAKNS
                     NGCTGSANFTLTAANPCSGVTITVNATVNNPTAPGATNGSIAATAGGGATPYTYSLNG
                     GAFQSSGTFSNLAAGAYTITAKDNNGCQGSANFTLTAPNPCNGVTITVNGTVTNPSSA
                     GASNGSIVATASGGTSPYMFSLNGGTFQSSGTFGNLVAGSYSITARDNNNCTGSANFT
                     LTNPNPCAGVTISVSNTVVQVTPCVSPVNGSITATASGGTAPYTYSLNGGAFQSSNVF
                     SNLNTGVYAVTAKDVNGCTGTSSNSVVSSLPAGPLFSAVKNVLQANCVTCHNASTPNG
                     GMDWTIDCNIVNNRERIKARAVDANPSAMPPTGLLPASERQKITDWINAGGRYTD"
     gene            complement(8255..8812)
                     /locus_tag="PK977_09940"
     CDS             complement(8255..8812)
                     /locus_tag="PK977_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRF18481.1"
                     /translation="MRKIVVAITGASGSIYAKNLLDKLVHIKEQWAELSVVMTENAKD
                     VWKTELDNDVYLHYPVRYFGTTDFMAPFASGSGQYDTMIIVPCSMGTLGRIASGVSND
                     LITRAADVVLKERRKLICVVRDTPYNLIHIRNMETVTLAGGIICPATPSFYSRPQTIE
                     DVAATVVDRVIDLAGLDIKTFRWGK"
BASE COUNT         2698 a         1775 c         1959 g         2426 t
ORIGIN      
        1 taaatacgga ataggtttct ggaaacccgg tgccggtatc attcaccagg tagtgctgga
       61 aaattatgca tttcccggcg gaatgatgat cggtacagat tcccacacgg taaatgcggg
      121 tggtttgggc atgattgcca ttggtgttgg cggagctgat gcctgtgatg tgatggcggg
      181 cctgagttgg gaattgctga tgccaaaatt gatcggagtg aagcttacag gaaaattaaa
      241 cggctggact gctccaaaag atgtaatcct gaaagtagca ggtatcctta ccgttaaggg
      301 aggaaccaat gccatcgttg aatattttgg agaaggtgca accagcatga gttgtaccgg
      361 aaaaggaacc atttgtaata tgggtgcgga gattggcgcc actacttcta ctttcggata
      421 tgatgaaagc atgagccgct acctgaaagc aaccggccgt gccgaagtgg ctgaagcagc
      481 cgataagata aaagaatacc tgacaggtga cgctgaagcc tatgccaacc ctgaaaaata
      541 ttttgaccag gttatagaga ttaaccttag tgaactggaa ccacacctga atggtccttt
      601 caccccagac ctggcaacgc cagtttctca aatgaaagag gctgctgcca aaaatggctg
      661 gcctaccaaa gtagaagtag gtctgattgg tagctgtacc aactcttctt atgaagatat
      721 cagccgtgcg gttagcctgg ctaaacaggt aaaagaaaaa ggactcaaac taaaatctga
      781 attcaccatt accccgggaa gtgaacaggt aaggtatacc attgaaagag acggctacct
      841 tgatacgttt gccaatatcg gtgctaccgt atttgccaat gcatgtggtc catgtatcgg
      901 tatgtgggac aggatgggtg cagaaaagca ggagaagaat actattgtac attctttcaa
      961 caggaatttt gcgaagagag cggatggtaa tcctaatact atggcctttg ttgccagtcc
     1021 tgaattggtc actgccctcg ccattgccgg ggaccttact tttaacccta tgacagatac
     1081 cttaaccaac gataaaggtg agcaggttaa gttagatcca ccatcaggtg atgaattacc
     1141 ggtgaaaggt tttgctgtag aggatgccgg ttaccaggct ccggctgaag atggcagcag
     1201 agtggtggta gatgtgaaac ccgacagtaa gcgtcttcag ttactttatc cgtttgcagc
     1261 atgggaaggc accgatatca aaggattgaa attactcata aaggccaaag gaaaatgtac
     1321 aactgaccat atcagtatgg ccggtccctg gctgaagttc cgtggtcacc tggataatat
     1381 cagcaataac ctgctgattg gtgctaccaa cttcttcaat gaaaagacag atagtgtgaa
     1441 gaaccagctg accggtgagt atggtactgt tcctaatacg caaagagcct acaaagctgc
     1501 tggtatcgga actatcgtag ttggtgatga aaactatgga gaaggttcca gcagggaaca
     1561 tgcagccatg gagccccgtc atttaggcgt aagagtggtg ttggtaaaat cgtttgcccg
     1621 cattcatgaa actaacctga aaaaacaggg aatgttggct atcacttttg cggataaaga
     1681 agattataac aaaatacagg aggatgatac gatcgatgtt attggtctga cttcattcca
     1741 gccgggtaag caattgcaat tagtgctgaa tcacaatgac ggcagcagtg aaaccattaa
     1801 tgtgaatcat agttataatg agcagcagat cgagtggttc aaagcaggag gggcattgaa
     1861 tgttatccgt gcagaatttg caaaaggtta atgaattaag atttcaaaat aaaggccggt
     1921 cattagcgac cggcctttat tttttagggt cttcctgcag aagttttgct gccggttata
     1981 atggaggcca gcagtctgcc atttggcagt tcagaagaag tataataaag tttcaaaact
     2041 gtattatttg cttttttaag actgatgata tctttgacct tacagctcat tcctgataca
     2101 ggattgttta catctgcaaa acgaatttct ttcagcactt tttcctggcc gtctttaatg
     2161 gtcagtaccc tttttttgcc aactcttccg caatggtgat acttaatagt gatttcatca
     2221 ttggcagagg catcggataa ttgaatgctc tgcggactgt tcagctggtt gccgnnnnnn
     2281 nnnntgcatg atcattttgt tgttcaggaa aacttcaaac ccttctccgc caggaggggt
     2341 ggagaaagaa aataaggtga atgccagcat cagcaataga gctgcttttg gaaatcttgc
     2401 tttcatggct gataatttta agattgaata aataatttat caggttaaaa ttagggctat
     2461 gccgggaggc atgctgttta ctttgacata agggttgttt cacctgataa aatgcaaaaa
     2521 gggagtgaac aatcactccc tttataagac ttaatggcta ctgttattat ttactcacca
     2581 tcacttttac attcagtggc tcattagtcc ctttcatcca taccctcagt gtatacatgc
     2641 cagaagaaac atagttgatg ttgagcttat tagtggnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgttgt ccaatacgat gttcagttga
     2761 ccgtcaggag acgggttagg gaataccacc acatttccgg tttgttccag tccttttacc
     2821 agcctgatat cgctatagct ggctttgtta tcaaggtcca cctggcggat gcggtattgg
     2881 cttaccgctt taagattatt aaaatctgta tagttgtaaa ataggttctc attactgttg
     2941 ccattagctg cctggctggc tatatatccc attacctgcc actcaccatt tcctgatttt
     3001 ctttcaatgt aaaatccctt gttgttcaat tcagttgcgg ttacccattt cagcattacg
     3061 ttttccttat tgcgccatgc agtgaatgag gcgaattgga ctggtaatgg tgtacaagaa
     3121 tttgtaatca gggcatagaa ttcattgggt cttgatggtg ttgttaccac aacccacaaa
     3181 gatctgtctg cctcaggctt ttgattggaa tatggcaaat agccctgaat accggactgg
     3241 tatttgtaag agtttgttgc atcgagtaaa gttgtcccgc tgttaacaat tatgttatct
     3301 gaaattgcat tgtagattcc atcaccatta tctattgcta ctttgtaatt gatgttcatg
     3361 tctgtattac taatagtata aacctcaaaa gcatacgttc ttggagtttg acagaataag
     3421 aatcctctca gaccgggatc gttcacataa aaaggaatat ttgtactaga gcaacctact
     3481 ttttgagatt ggtcagcttg actttcccac gcatccgctg tcagtgattg aggaatacta
     3541 catccttgtg gtaatgttat tgtaagtcct cttatggtgt atctgtcagg tgttccaaca
     3601 gtttcagtta ttgtatagct gctttggaag cctggcgttg taggatctgg taaatatgta
     3661 agctgaggct ccagtgcact accctggtga aagaaaccaa atgttgtcaa tgagtttatc
     3721 aggtatccat taccattacc acttgttgaa aaggttggtg gtttgtcata actaaaattt
     3781 cctgtatata aacttgttgg ccagatatgt acccaaaaat actttccacc gggattatgt
     3841 tgaatgtcaa aatagaaatc aaaaactata acacaccgtc ctaattgatc tgaatatgtt
     3901 gttttaacat ctattccaat attgccaaaa taacaaggat cggcttttac attgttaatt
     3961 gaacctccca acgccaccat tgcgattatg agtataatct ttttcatgac tgaggatttt
     4021 agggtgacga atcattagct gattcaaaat ttattgacct ttttcaggat aaaggatttc
     4081 aatgtccttt ggtcattgag gagaggaaat atagaatgtc agctaaatgg ccaggatctt
     4141 atagtagaat tacttccatg ggttgtgatc ctttaagaat gaacctggtt ctaaattaat
     4201 atcatctact tacctgctat tatgatttat gagaaagtaa acaatgatat tcgtcatgta
     4261 ccacaatctt ttttccctaa gtaaaatgtg atgaaattga ttactatttt tacgatctta
     4321 tgcagcgatt aaaaccgggt gtagatatag tactggatat cctaaatgca gttgcagaag
     4381 cgcagcccca ttctgagttt gcccaaagta tcctgcggca gtaccaggaa aggggtggat
     4441 taagtaaaaa acaactgcaa ggtctttatg ggaaagctca gaaactacaa tccataccac
     4501 cgggcaaact ggctacactc gaagcgataa ttctaaaaaa gcataccaaa gaacgttccg
     4561 ggatacctgc caccagtccg ctttatcaaa aagatagtat taccggtcaa ttaattgatt
     4621 cgatcttaaa gcaattccct tcccataaaa gagtgttgta tctcaaatca aaatatgata
     4681 ataatgaggt acttagctct gttgaacttt ctgaactgaa tagattcagt aaacttttga
     4741 aataaatagt tactttatat ctgctggtgg caaccaattc atgcctgaac ccgattaatt
     4801 gaccaaacag tacacacaat tgctatctgc tgaggaacta acctttcatg tggaggcttg
     4861 cagccgaaac agcagggaga gccaaaagat catttatggt tctttctatg gctatgctat
     4921 ggcaatttgc gaccgatata ccaataaaca ggaggatgct gttgaaatac tgaatgatgg
     4981 attcctgaag gtcttcaagg acattcataa tttcaagccg gcatatgccg atttgatcag
     5041 ttcatttaag ggatggctcc gtaagatcat gatctatacg gctatagacc atttccggaa
     5101 acaccataaa catcagttgg tcagtaaact ggatgatgtc acttaccagt taccgcctgt
     5161 ctatgatgat gcggttgata aattatccta cgaggagatc ataagagcgg tgcaggaatt
     5221 gtcgcctgct tacagagccg ttttcaatct ttttgttata gaagggttta gccatgaaga
     5281 aatagctgaa cacctgggca tttcaacagg tacatcaaaa tcaaacctgt tcaaagcaag
     5341 gttgcagctg caaaaaatat tatttaagaa ctatcatata caagttgcca aaaatgcagt
     5401 ctgaagaatt tgataaaaag gtaagagagg cagccagcca tcaccatccc gcctacgatg
     5461 aacaggcatg ggataagatg cacaaactgc ttgacaagca tcttccagaa gagaaagatg
     5521 accgcagaag gataatattc tacctgttat ttttcctttt attgggtggc ggcatatggt
     5581 tacttttggg aaaactggga gaacaaccac aaaagcccat ttccacccag gccaggcaaa
     5641 aagagaccca tacaagttcc ggcggcaata atgtaacagg tgaaattgaa ggcagaaata
     5701 atcaaccccg gcaaaccacc acagtttcag ctaaggaagt aacaatagca ggtacacctg
     5761 ccaatacaac aaaaacgacc agtcaaaatc agcattcaaa ctttttgtca aataaaaaga
     5821 tgaataatcc ggtacctgtt acggataaga ttattgctgt gctttcaggc aaagaatcaa
     5881 ataagaatat aaatgcaggt aaagaagttc aaaagaacag cgataaagat gagctgtcgc
     5941 ctgatcagca gcaaaaggtc aaagaagagt tggcaaacag gaaggaaagc atcttacccg
     6001 gtgtaatcac aaataaaaaa acagaaaatc agccttcagc aaatgaaaaa gcggatacaa
     6061 ctattctgaa caaatccata gtgcaggata gtgttgctgt taattctgtg aataaaaccg
     6121 ctaagaagca ggcggacaaa aaggacaaag acaaaaagac aaagaataaa aaaagcaatt
     6181 tcttttttgt ttcactttca gcaggaccag atgtgagtta caccaccggg ggtcagttag
     6241 gcactacaaa attgacaggg ggcatagggt taggatatac ctataagaac aggatatcag
     6301 tccgaaccgg gtttttcagt ggcaggaaag tatatgctgc ttcaccggat gcctataacc
     6361 ccccggcaat tttttggaat tattatcctt acctggaaaa agttgatgcc aactgtaaag
     6421 tgtatgaaat tcctgtattg gccagttatc attttggtaa accgggtaag cagcattggt
     6481 ttgtttcagc gggattgtct tcacttataa tgaaacagga agattatcac tatgcatata
     6541 agtatacacc caacggagcc acactttaca gggactggac agtgaaaaat gaaaacaaac
     6601 atttcttctc tgttgctact gtttccgggg gttaccagaa aagtatcggt aagtcaatta
     6661 catttacggc tgagccatat ttaaaacttc cgctaggtgg cgttggttat ggcagagtaa
     6721 aactcaacag taccggaatt ttattcagtg tatctgtaaa accatttcag caaaacaagc
     6781 agacggataa aaagccataa acagagtagc aggcaacctt gtacagtgtt gcagcgattc
     6841 aaataaaaaa ctgtttatga agaaacactt tattctgctg cagttgttgt ttgtaatcgc
     6901 atctggaatt ttattcaaca gttcctgtag caaaggaggt ggcaatactc ctcctccggc
     6961 taatccctgc actggcgtca cggtagttgt caatggaacc gtagcaaatc caactgctcc
     7021 cggtgccagc gatggaagta tcattgtaac agcaaccggt ggttcgggat ttaccttcag
     7081 cttaaataac ggtgcatttc aaagttcagc cacattttcc gggctagcag caggagctta
     7141 tacggttacc gcaaagaata gcaatggttg taccggatcg gccaatttta cattgaccgc
     7201 cgccaacccc tgttcaggcg ttacaattac tgtgaatgcc acagtgaata atcctacagc
     7261 accgggagct accaacggaa gtattgccgc aacagccggt ggtggcgcta caccatatac
     7321 atacagttta aacggtgggg cttttcagtc atccggaaca ttcagcaacc tggcagcagg
     7381 agcatatacc attactgcaa aagataacaa tggttgccag ggttcggcca actttacatt
     7441 aacagctccc aatccttgta atggagtaac tattacagtt aacggaacag tcacaaaccc
     7501 ttcatcagcc ggggccagca atggaagtat tgtggccact gccagtggcg gaacttctcc
     7561 ttacatgttt agtttgaacg gaggtacatt tcaatcatcc gggacattcg gtaatcttgt
     7621 tgcaggatct tatagcatca cagcaaggga taataataac tgtactggtt cggcaaattt
     7681 tacattgaca aatccaaatc cctgtgctgg tgttacgatc agtgttagca atacagtagt
     7741 acaggttact ccctgtgtaa gcccggttaa cgggtctatc accgccactg ccagtggtgg
     7801 aacagcaccc tacacatata gcctgaatgg cggcgctttc cagtcatcca atgttttttc
     7861 caatctcaat accggggtat atgctgtgac ggcaaaagat gtgaatggct gtaccggcac
     7921 atcttccaac agtgtggtaa gttcattacc ggcagggcct ttattttcag cagtaaaaaa
     7981 tgtgttgcag gcgaattgcg tcacttgtca taatgcttct acgccaaacg ggggaatgga
     8041 ctggacgatt gactgcaaca ttgtaaacaa cagggaaaga attaaagcaa gagcagtaga
     8101 cgcaaatcct tctgccatgc cgcctacagg attgcttcct gcatcagaaa gacaaaaaat
     8161 cactgactgg atcaatgcag gtggccggta tacagattag aatggtggat aataatgtgt
     8221 gtaatgtaaa agaggctctc caatgggggc ctctttattt tccccagcgg aaagttttta
     8281 tgtcaagccc agcaaggtca attacccggt caacaacggt ggcagcaaca tcttcaatgg
     8341 tttgcgggcg gctgtagaag gatggtgtgg caggacaaat aatgccacca gccaatgtca
     8401 ctgtttccat attgcgaata tgaatgaggt tatacggagt atcacgaaca acacagatca
     8461 gtttccttct ttcctttaat accacatctg cagcccgggt aataagatca tttgaaacac
     8521 cggaggcaat tcttcccaaa gtccccatgg agcagggaac aataatcatt gtatcatact
     8581 gcccggagcc ggaagcaaaa ggcgccataa aatcagtggt gccgaaatag cgaaccgggt
     8641 agtggaggta aacatcattg tccagttctg tcttccagac atcttttgca ttttctgtca
     8701 ttactacaga cagttctgcc cattgttctt tgatatgaac cagcttgtcc aataaatttt
     8761 ttgcatatat cgaaccactg gctccggtga tggcaacaac aatttttctc atagtttgat
     8821 aacctgctaa agaagcaaaa gttgccggaa gttagaagaa tttgtgcaaa cgtttgcaat
     8881 gaaattgtag tagaacggct acacggtaaa aaatcgacaa actaca
//
LOCUS       DAORLS010000116         8870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4268_length_8870_cov_2.347136, whole genome shotgun sequence.
ACCESSION   DAORLS010000116 DAORLS010000000
VERSION     DAORLS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4268_length_8870_cov_2.347136"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PK977_09945"
     CDS             <1..281
                     /locus_tag="PK977_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18482.1"
                     /translation="NGTKLLRSYADENKVEIVLENRKYRLEILAHRDNATELASPILG
                     FMNGRIEESMTAALDVTLTDKKAGNVLLQDTGKNAGLEVAGMINEIMV"
     gene            complement(282..2021)
                     /locus_tag="PK977_09950"
     CDS             complement(282..2021)
                     /locus_tag="PK977_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18483.1"
                     /translation="MKRQFFTVLAALITFSVSAQTYRITWGEEIKLKKGTADLDIVAA
                     DNTGLFFTETRRKMTGYFVIGATYGNAIKLMKLDKNFGEVFDKEYKKELKGLEYHSFQ
                     PLENDLYMFASDYEKKSRQFKILGAKVDKNSGDIPGDFVELGSYELESKRDDYEMKVT
                     RVQNGKAFLMVSNISGKDRVSLGISILDKSFKKKENTVINLSFNPDHYQLQDVQYTKS
                     NKIVLLGKEYEEAQYGKKKRKRLVFKQYVLGIYNMNGGKEKEVVINSDDRYVISGKLI
                     ETPAGEMLLAGFYSNVAKKDDLNGFFINKVDPEKGEMTLSSFKEINAGMLGKSFEDEA
                     DEDDEIKANKKQAQKAKAEDDEEEFPNKFIIKSVDINPTDNSIVITSEVSRYSYYFFT
                     DSYYNNTTRTWTRSTTHVHRFTNQDILIINADKDGSIKWLNALPKSQLEEIRTSNNTS
                     GNGIFFGGNDQGSYFAAAGGMPFYSSYSSLINNNKLVIIMNDHTSNNVNPEYGNRVKT
                     VSNFRKRSNVYGISIDLATGAMTRKIIASNNEETILMPRHAFVVKNELFVPSWRQKIL
                     AKTELKFAKISVK"
     gene            complement(2081..2425)
                     /locus_tag="PK977_09955"
     CDS             complement(2081..2425)
                     /locus_tag="PK977_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRF18484.1"
                     /translation="MNIETLREYCLSKPGAEETLPFGPDTLVYKVGGKAFLLMGLDND
                     DFRFNVKCDPDKAIELREEYGCVLPGYHMNKKHWNTIVVDGSVSSQLLKDWIDHSYDL
                     VVASLPGKKKKQ"
     gene            complement(2435..>2897)
                     /locus_tag="PK977_09960"
     CDS             complement(2435..>2897)
                     /locus_tag="PK977_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18485.1"
                     /translation="IYGEQNVKDERICGPECIDSLDVTQVYPGTSKAFTVYWKEKQYH
                     KKIEMIECYTEEAPYRTASDIGIGTSLQYLLELNGKKITFYGFGWDYGGGIVSFNGGK
                     LEKSNLHYTLDMTYDTTGNSIMGDAEFNTDMPEVQKHIGKIFVDKIVLSFD"
     assembly_gap    2898..2915
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2916..3103)
                     /locus_tag="PK977_09965"
     CDS             complement(<2916..3103)
                     /locus_tag="PK977_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18486.1"
                     /translation="MRKLILLIPALALLLSCQSKNEAKKTDTSNVDSAGKETHYYKID
                     LIQITDSAWGSVTRNTNF"
     gene            complement(3129..3518)
                     /locus_tag="PK977_09970"
     CDS             complement(3129..3518)
                     /locus_tag="PK977_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF18487.1"
                     /translation="MSRTNYSSGAKWEDIVGYSRAVRVGNIIEVTGTVAVDENNQLVG
                     KDNAYAQTRFIIEKIERVLKRAGSSLKDVVRTRMYVTDIARWEEYGKAHGEFFKDIKP
                     CTTMVEVKGLIAPEYLIEIEATAIISE"
     gene            complement(3520..4242)
                     /locus_tag="PK977_09975"
     CDS             complement(3520..4242)
                     /locus_tag="PK977_09975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF18488.1"
                     /translation="MASFPVSNILFLDIETAPQYPDYNDMPEEWKQLWDTKSASLLKY
                     HEGQTNESLYPRAGIYAEFGKIICISCGVLQGADGHKKIVLKSFYGDDEKLLLHQFSE
                     MLNKWSAGEQKFLCAHNGKEFDFPYLCRRMIINNLPIPSLLNIAGKKPWEVTHLDTLE
                     LWKFGDFKSFTSLNLLAHTLGIPTPKDDIDGSMVWAVYWKEKNLERIVTYCQKDVVTV
                     AQIYLRMNGDPLIKQENIEIKN"
     gene            complement(4311..5393)
                     /locus_tag="PK977_09980"
     CDS             complement(4311..5393)
                     /locus_tag="PK977_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRF18489.1"
                     /translation="MNKHHYVAIMAGGIGSRFWPMSRTNFPKQFLDILGTGRTLIQQT
                     WDRYSKLVNKENIFIITAQEYVNIVKKQVPDIPEENILAEPSRKNTAPCIAYIAFKLL
                     KKDPRALMIAAPADNLILETDEFLKTAKKAFSFVDHINALLTIGVKPSYPNTGYGYIQ
                     HDTTEAAPDIFKVKTFTEKPNLELAKAFIASGDFLWNAGIFTWKVKNIITALEKHEPE
                     LYELFAAEKDKFNTDAEREAVENIYPQCTNISIDFAVMEKADNVFVIPASFGWSDLGT
                     WNSAWENKDKDYFGNAVVGKHVMVVDANNCMVHVPDNKLVLLQGLKDYIIIDTKDVLL
                     ICKKDKEQEIKQYVAEVKRNMGDKYL"
     gene            complement(5472..6434)
                     /locus_tag="PK977_09985"
     CDS             complement(5472..6434)
                     /locus_tag="PK977_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRF18490.1"
                     /translation="MPIDIKHIALRTIEMEARSIAGLSVSVNEDFVKAVQVIASCTGR
                     VVVSGIGKSAIIAQKIVATFNSTGTPSLFMHAADAIHGDLGMVQQDDVVMVISKSGES
                     PEIKVLVPLIKNFGNTLISIAGNKNSYLAKNANINLDTTVDQEACPNNLAPTTSTTAQ
                     LVIGDALAVCLMEMRGFQADDFAKFHPGGMLGKKLYLRVSDLADGNEKPRVLSTQSLK
                     EVIVEMTAKRLGLTAVVDEDNNLLGIITDGDLRRMLEKNVAIDAVKAGDIMTKHPKAI
                     GPDELAIEALDMLRKNEISQLAVVQDKKYLGIIHLHDLIREGLI"
     gene            6866..7495
                     /locus_tag="PK977_09990"
     CDS             6866..7495
                     /locus_tag="PK977_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF18491.1"
                     /translation="MGTTVKKAATKTTKSANGKAATVKEAKTKTPKNGSVKTKFDLHK
                     ALQEYFGFDKFKGTQEKAIESLLAGHDTFVIMPTGGGKSLCYQLPAMVSEGVAIIVSP
                     LIALMKNQVDLVRGYSSNDEVAHFLNSTLTKKEIKEVHDDLLTGKTKMLYVAPETLTK
                     QENLEFFSDLTISFFAVDEAHCISEWGHDFRPEYRRLREMMIQIILMHR"
     gene            7492..>7889
                     /locus_tag="PK977_09995"
     CDS             7492..>7889
                     /locus_tag="PK977_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRF18492.1"
                     /translation="MIALTATATPKVQSDIIKNLDLRNPNVFISSFNRPNLYYEVLPK
                     IKKAQTDEHIVRFIKGTKGKSGIIYTLNRKTTEELADILMANGIKAVAYHAGLDSKLR
                     ADRQDQFLNEDVQVICATIAFGMGIDKPDIR"
     assembly_gap    7890..7903
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7904..>8870
                     /locus_tag="PK977_10000"
     CDS             <7904..>8870
                     /locus_tag="PK977_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HRF18493.1"
                     /translation="HYNIPKSIENYYQETGRAGRDGLEGKCILYYSHKDVSKLEHLMR
                     DKPLSEREVGAQLINETVAFAETGVCRRKVLMSYFGEEYEKENCGQCDNCKHPKERIE
                     AKDETVIVLKAIKELDERFATDYVVQIVSGKLTPQIQMFRHDGLNAFASGNDRDAHFW
                     NSLIRQLLLEGLLSKDIEEYGVLKFTKKGEAFLKKPKSFQIVLNKLYDDANADDEEGT
                     ETTTGAASDERLFEMLKDLRQKEAKKKGLPPFVIFLESSLQDMSTFYPITLEGLEKCQ
                     GVSKGKALKYGRPFVDLIAKYVEENNIERPDDFVMKSVVNKSGNKV"
BASE COUNT         2412 a         1985 c         1802 g         2639 t
ORIGIN      
        1 ataatggtac aaaacttctc cgttcgtatg ctgatgaaaa taaagttgaa atagttctgg
       61 agaaccgaaa ataccggctg gaaatcctgg cgcatcgaga caacgccact gaactggctt
      121 ctcccatact tggtttcatg aatggccgta tcgaagaaag tatgacggcg gctcttgatg
      181 tgacactcac cgataaaaaa gctggaaatg tattactgca ggataccggt aaaaatgccg
      241 gccttgaagt ggccggcatg atcaatgaaa ttatggtctg attacttcac agaaatcttc
      301 gcaaacttca actcagtttt tgctaatatt ttttggcgcc aggatggaac aaacaactca
      361 ttctttacca caaatgcatg tcttggcatc aggattgttt cttcattatt ggatgcaatg
      421 atctttcttg tcatagcgcc tgtggccagg tcaattgaaa ttccataaac attactccgc
      481 ttcctgaaat tggaaacagt ctttaccctg ttcccgtatt ccgggtttac attattgctg
      541 gtatggtcat tcataatgat caccagttta ttattattaa taagactgga ataggaagaa
      601 taaaacggca ttccgccggc agcggcaaaa taacttccct ggtcatttcc cccaaagaaa
      661 ataccattgc cggatgtatt gttgctggta cggatctctt ccagctgtga ttttggcaaa
      721 gcgttcagcc atttgatact tccgtccttg tctgcattaa tgatgaggat gtcctggttg
      781 gtaaaccggt gaacatgggt agttgaacgg gtccatgttc gggtagtatt attgtaatag
      841 gaatctgtga aaaaataata ggaatacctg gatacctcac tggtaataac gatagagtta
      901 tctgttggat taatatcaac agactttata ataaacttgt tgggaaactc ttcctcatcg
      961 tcttcggctt tggctttttg agcctgcttt ttattagctt ttatttcgtc atcctcgtca
     1021 gcttcatcct caaaggattt tcccaacata ccggcgttga tctctttgaa agagcttagc
     1081 gtcatttcgc ctttttcagg atccacttta ttgatgaaga agccattgag atcatctttt
     1141 ttggcgacat tactataaaa gcctgccagt aacatttctc cggcaggtgt ttcaatcagc
     1201 ttcccgctga tgacatagcg gtcatcagag ttaatcacca cctctttctc ttttcctccg
     1261 ttcatattgt aaatacccag cacatactgt ttgaacacca gcctttttct ttttttcttg
     1321 ccatattgcg cctcttcata ttcttttccc agcaagacaa tcttattgct tttggtatac
     1381 tgcacatcct gcaactggta atgatcggga ttaaaggaga gattgataac tgtgttttct
     1441 tttttcttaa aacttttatc aagaatgctg atacccagtg aaaccctgtc ctttcccgaa
     1501 atattactga ccattaaaaa tgcctttcca ttctgtaccc gggtaacttt catttcgtaa
     1561 tcatctcttt tgctctccag ttcatagctg cccagctcca caaaatcacc gggtatgtca
     1621 ccgctattct tatccacttt tgcccctaat attttgaatt ggcggctctt tttctcatag
     1681 tcagaagcaa acatgtacag gtcattttcc agcggctgaa agctgtgata ctccaggcct
     1741 ttcagttcct ttttgtattc tttgtcaaaa acctccccga agttcttatc cagtttcatt
     1801 agtttaatag cattgccata ggtagcgcca ataacaaaat accccgtcat cttcctcctg
     1861 gtctcagtaa aaaacaggcc ggtattatca gccgctacta tatccaggtc agcagttcct
     1921 tttttcagtt tgatctcttc tccccaggtg attcggtagg tttgggctga aacagaaaaa
     1981 gtgatcaatg cggccagtac ggtaaaaaat tgtcttttca ttaagtataa ttaggccgct
     2041 aaaataggag aattgatatt gatgcgattg caaaattttg ttactgtttc ttctttttgc
     2101 cgggcaagga tgccactaca agatcataag aatgatcgat ccagtctttt aatagctgag
     2161 aagaaactga gccgtctact acaatggtgt tccagtgttt cttattcatg tggtatccgg
     2221 gcagcacaca gccatattct tcccttaatt caattgcttt gtccggatca cattttacat
     2281 tgaagcgaaa gtcatcgtta tccaggccca ttagcagaaa ggctttccct cccactttat
     2341 acaccagggt atccggacca aaaggaagtg tttcttccgc tcccggtttg gagagacaat
     2401 attccctgag ggtttcaata ttcattttct aaaatcagtc aaaggatagc acaatcttat
     2461 ccacgaatat tttaccgata tgtttctgta cctccggcat gtccgtattg aattctgcat
     2521 cacccataat ggagttaccc gtcgtatcat acgtcatgtc aagggtataa tgcaggtttg
     2581 atttctccaa tttacctccg ttgaaagaaa cgataccgcc gccatagtcc cagccaaaac
     2641 cgtaaaaagt aattttctta ccattcagct ccagcaggta ctgtaatgaa gtgccgatgc
     2701 caatatccga agcagtcctg tatggcgctt cttccgtgta acattcaatc atttcgatct
     2761 ttttgtggta ttgcttttct ttccagtaca cggtaaaggc tttgctggta cccgggtaca
     2821 cttgtgttac atccagcgag tcgatacact cgggaccaca aatacgttca tctttcacat
     2881 tttgctctcc atagatcnnn nnnnnnnnnn nnnnntcgaa attggtattc cttgttacag
     2941 aaccccaggc agagtcagta atctgaatta gatcgatttt gtaataatga gtttctttgc
     3001 cagcactgtc cacattgctt gtatctgttt tcttcgcttc attcttactt tggcaactta
     3061 aaaggagtgc cagggccgga ataagcagaa tcagctttct cataataaaa attttttgca
     3121 ccttaaagtt actcactaat gatagcggtt gcttcaattt ctatcagata ttcgggggct
     3181 atcagtcctt ttacttccac catcgtggta cagggtttta tgtctttgaa aaattcgcca
     3241 tgtgctttgc catattcttc ccaacgggca atatctgtaa catacattct tgtacgcacc
     3301 acatccttta atgaagaacc ggctcttttt aaaacccgtt caattttttc aatgatgaac
     3361 cttgtttgag catacgcatt atcttttcca accagctggt tgttttcatc tactgctact
     3421 gttccggtca cttcaataat attgccgacc ctcactgcac ggctgtagcc gacaatatct
     3481 tcccatttgg ccccggaaga ataattggtt ctgctcatat cagtttttta tttcaatatt
     3541 ctcttgtttg atcagcggat caccattcat ccggaggtat atctgcgcca cggtcaccac
     3601 atctttctgg caataggtaa caattctttc caggttcttc tccttccagt aaacagccca
     3661 gaccatgctg ccgtcaatat catctttggg tgttgggatg cccagcgtat gggctaacaa
     3721 gttgagggaa gtaaaacttt taaagtcacc aaacttccaa agctccagtg tatccaggtg
     3781 cgtaacttcc cagggctttt ttccggcaat attcagcaaa gagggtatcg gcaagttatt
     3841 aatgatcatc cggcggcaga ggtaaggaaa atcaaactct ttaccattat gtgcacacaa
     3901 aaatttttgt tcgcctgccg accatttatt cagcatttcg ctgaactggt gtaacaataa
     3961 tttttcatca tcgccataaa agctttttaa aacaatcttt ttatgaccat cggcaccctg
     4021 cagtacacca caactgatac aaatgatctt gccaaactct gcataaatac cggcacgggg
     4081 gtacaatgat tcgttggttt gtccctcgtg atattttagt agtgaggctg atttagtgtc
     4141 ccacaattgt ttccattcct ccggcatatc attataatcg gggtattgcg gtgctgtttc
     4201 tatatccaaa aacaggatgt tacttaccgg aaagctggcc atggttaatg tttatgtcct
     4261 aaaaataaaa aatcccgctg tagcgggact accactttgt ggtaattctt ttacaggtat
     4321 ttgtcaccca tatttcgttt cacttctgca acgtattgct ttatctcctg ttccttgtcc
     4381 tttttgcaga tgagcaatac atctttggta tcaatgatga tgtaatcttt taatccctgc
     4441 aataatacca gtttgttatc gggtacatgt accatgcaat tattagcatc taccaccatt
     4501 acatgcttgc ctaccaccgc attgccaaaa taatctttgt ccttgttttc ccaggcgcta
     4561 ttccatgtgc caaggtcgct ccagccaaaa gatgcaggaa tcacaaatac attatctgct
     4621 ttttccatca ctgcaaaatc aatagaaata ttagtgcact gggggtaaat attttcaaca
     4681 gcctctcttt ctgcgtctgt attaaacttg tctttctctg cagcaaacaa ctcatacaac
     4741 tctggttcat gtttttccag cgctgtaata atattcttga ccttccaggt aaatatgccg
     4801 gcattccaca aaaaatctcc gctggcaata aaggctttgg ccagctccag gttgggtttt
     4861 tccgtaaacg ttttcacttt gaaaatatcc ggcgctgctt ctgttgtatc atgctgaatg
     4921 taaccataac ccgtattagg ataagagggt tttacgccta tagtgagtaa tgcatttatg
     4981 tgatcaacaa agctgaaggc cttttttgcc gtctttaaaa actcatccgt ctccaggata
     5041 aggttatccg ccggtgcggc tatcatcagt gccctgggat cttttttcaa cagcttgaat
     5101 gcaatgtagg ctatacatgg ggctgtattt tttcgggaag gttctgccag gatattttct
     5161 tccggaatat cgggtacttg tttttttaca atatttacat attcctgtgc tgtgatgatg
     5221 aagatatttt ctttattcac cagtttgctg tagcggtccc atgtttgttg aatcagcgtt
     5281 cggccggttc cgagaatatc gagaaactgc ttgggaaaat tggtacggct catcggccag
     5341 aaacgactac cgattccccc ggccattatt gctacatagt ggtgtttgtt catctgtaat
     5401 agttgaaatg ctgataagtt gggtttgaca tgctgctatc ttgttaacct gtcaaccttt
     5461 taacttgtca actatattaa tccttccctg atcaggtcgt gtaaatgtat aattcctaaa
     5521 tactttttat cctgtacaac tgccagttgt gaaatctcat ttttgcgaag catatccagt
     5581 gcctctatcg ccagttcatc cgggccaatt gcttttggat gcttcgtcat aatatctcca
     5641 gcctttacag catcaatcgc aacgtttttt tccagcattc gccttaaatc tccatcggtg
     5701 atgataccta aaagattatt gtcttcatcc accacagcag ttaaacccaa tcgctttgct
     5761 gtcatttcaa caatcacttc ttttaatgat tgggttgaca gcaccctggg cttttcatta
     5821 ccatccgcca ggtctgatac cctcaggtaa agctttttac ccagcattcc tcccggatgg
     5881 aatttggcaa aatcgtccgc ctgaaatcct cgcatttcca tcaggcaaac agccagcgca
     5941 tctccaatta ccaattgtgc tgtagtgctg gtagtggggg ccaggttatt cggacatgct
     6001 tcctggtcta ctgttgtgtc caaattaata ttggcgtttt tggccaggta agagtttttg
     6061 ttcccggcga tactgatcag tgtattgcca aagtttttga tcagcggcac cagcactttt
     6121 atctcagggc tttcaccgct cttgctgatc accattacca catcatcctg ctgcaccatt
     6181 cccaggtcgc cgtgaatggc atcggccgca tgcataaaaa gggaaggagt gcctgttgag
     6241 ttgaacgttg ccactatttt ctgcgcaata atggcactct tgccaatgcc gctcaccacc
     6301 acccggcctg tacaggatgc aataacctgt acagccttca caaaatcctc gttgacagat
     6361 accgacaggc cagctattga ccgtgcttcc atctctatgg ttctgagggc tatgtgttta
     6421 atgtcaattg gcattggctt gaatatcgcc ttaatacccc tgcgataagg ccgcaaattt
     6481 agccttttta gtatggaata accatgcgat tggccggctg ttgaatttta acggttttgc
     6541 ccggaaaaac acataattca gttgtaaatt cgcaaccctt ccgccaaaag cggaaacacc
     6601 tcaacaacta aaaaggggaa agaagttgtg cgactctgct gatgcggagc ccggtaaaag
     6661 aaatcaaccc gctgaatccc cggttatagg ctgagaaatt actcaacgat gttgtttgcc
     6721 tttgatcaat ttaaaggcag gctgtgcaaa aaataaggca ggcctgtccc ggcattcggc
     6781 taaaattgag tatcttaaac aactgacgtt ataccatatt ggtatccata gtccggaagg
     6841 actcctatga agaaaaactt gaaaaatggg aacaacagta aaaaaggctg caaccaaaac
     6901 caccaaatca gctaacggaa aagcagccac ggttaaggaa gcaaagacaa aaacgcctaa
     6961 aaacggatca gtcaaaacta aattcgacct gcataaagca ttgcaggaat attttggctt
     7021 tgataaattc aagggcacac aggaaaaagc gattgaatca ttgctggccg gtcatgatac
     7081 atttgtcatt atgccaaccg gcgggggcaa aagcctgtgc taccagttgc ccgccatggt
     7141 gagtgagggg gtggccatca ttgtatcacc gcttatcgcc ttgatgaaaa accaggtgga
     7201 cctggttcgg ggttacagca gcaatgatga agtagcccat tttctcaact ctacactgac
     7261 caagaaagaa ataaaagaag tacatgatga tttgcttacc ggcaaaacca aaatgcttta
     7321 tgtggcaccg gaaacgctga ccaagcagga gaaccttgaa ttcttcagcg atttgaccat
     7381 ttctttcttc gcagtggatg aagcacactg tatctccgaa tggggacatg atttccggcc
     7441 tgaataccga cgcttgcggg agatgatgat acagataatc ctgatgcacc ggtgattgca
     7501 ttaactgcaa ctgccactcc caaagtgcag agcgatatca tcaagaacct ggacctgcgc
     7561 aatccgaatg tttttatttc ctcctttaac cgccctaatc tttattacga agtattgccc
     7621 aaaataaaga aggcgcaaac ggatgaacac atcgttcgtt tcataaaagg cacaaaaggt
     7681 aaaagtggta ttatatatac acttaaccgg aagaccactg aagaactggc tgatatttta
     7741 atggccaacg gcattaaagc cgttgcctac cacgccgggc ttgacagtaa actgagggca
     7801 gacaggcagg accagttcct gaatgaagat gtgcaggtga tctgtgctac tattgccttt
     7861 ggtatgggta ttgacaagcc ggatattcgn nnnnnnnnnn nnncactaca atatcccgaa
     7921 gtccattgaa aattattacc aggaaaccgg ccgtgcaggc cgtgatggac tggaaggaaa
     7981 gtgtattctt tattattcac ataaagatgt aagcaaactc gaacacctga tgcgggataa
     8041 gccactgagt gaaagagagg taggggcgca gctcatcaac gaaacagtag catttgcgga
     8101 aaccggtgtt tgccgccgca aagtgctgat gagttatttt ggtgaagagt atgaaaagga
     8161 aaactgcgga cagtgtgata actgcaagca tccgaaagaa aggatagagg ctaaagacga
     8221 aacggtgatc gtactaaaag ccatcaaaga actggatgaa cgatttgcta cggattatgt
     8281 agtgcagatc gtcagcggaa aactgacccc tcagatacag atgttccgcc atgacgggtt
     8341 gaatgcattt gccagcggta acgacaggga tgcccatttc tggaattcgc ttattcgcca
     8401 gctattgctc gaaggtcttt tgtcaaaaga tattgaagaa tatggtgtgc tgaagtttac
     8461 caagaagggg gaagcctttt tgaaaaaacc gaaatcattc cagatcgtac tgaacaagct
     8521 gtacgatgat gccaatgctg atgatgagga aggaacagaa actaccaccg gcgctgcatc
     8581 agatgagcgg ctgtttgaaa tgctgaaaga cctgcggcaa aaagaggcga agaaaaaagg
     8641 attacctccc tttgtgattt tcctggaatc ctcgttgcag gatatgtcta ctttctatcc
     8701 catcacactg gaaggactgg aaaaatgcca gggggtgagt aaaggaaagg ctttgaaata
     8761 cggaagacct tttgtggacc tgatagccaa gtatgtggaa gaaaacaata ttgaaaggcc
     8821 ggatgatttt gtgatgaaga gtgtggtgaa caagagcggc aacaaagtgt
//
LOCUS       DAORLS010000117         8813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4297_length_8813_cov_2.212606, whole genome shotgun sequence.
ACCESSION   DAORLS010000117 DAORLS010000000
VERSION     DAORLS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4297_length_8813_cov_2.212606"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PK977_10005"
     CDS             <1..637
                     /locus_tag="PK977_10005"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRF18494.1"
                     /translation="KVNGELVTEPGHKVSTKDDIRVNGKKIFLAKNLVYILLNKPKDY
                     ITTTDDPQGRKTVLDIIGRATNERVYPVGRLDRNTSGVLLLTNDGELAQRLTHPSNEV
                     KKVYHVTLNKPLEKKDFDQILNGVVLEDGLANVDTLAYADSKDKTQIGVEIHSGRNRI
                     VRRIFEHLGYDVKGLDRVMFAGLTKKNVDRGKWRFLTEKEVRDLKHFKKGK"
     gene            668..1393
                     /locus_tag="PK977_10010"
     CDS             668..1393
                     /locus_tag="PK977_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRF18495.1"
                     /translation="MKTTNWVIEETENWVAVNKPSGLLSIPDREGKEVSLKAILKEKY
                     GDIFTVHRLDKDTSGLILFAKNEEAHKHLSKQFEERRTKKIYQGLVIGCPEKQQGSID
                     SPIAEHPAQNGIMIIHRKGKESLTDYEVLEDFGIYSFLQFRIHTGRTHQIRVHMKDIG
                     HPIVCDNLYGDGKPILVSSLKSKFKLSKNEEEERPILNRVALHAYQLSLIDINGSLVE
                     LEAPLHKDMQASLQQLHKRKKGK"
     gene            complement(1432..2070)
                     /locus_tag="PK977_10015"
     CDS             complement(1432..2070)
                     /locus_tag="PK977_10015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRF18496.1"
                     /translation="MGLRLGDIAPNFKAKTTAGEIDFYEYLGDGWGILFSHPADFTPV
                     CTTELGKTALLAEEFAKRNVKVLAVSVDPLEKHQGWVKDINETQHCNVTFPIIADDDR
                     NVATLYDMIHPNASETFTVRSLFVIGPDKKVKLMITYPASTGRNFYEVLRVVDSLQLT
                     AGYSVATPADWKSGEDVIVVPAISTEDAVKKFPKGIKVIKPYLRYTPQPDLK"
     gene            complement(2121..2528)
                     /locus_tag="PK977_10020"
     CDS             complement(2121..2528)
                     /locus_tag="PK977_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRF18497.1"
                     /translation="MLSKKSQYAFKALTYLTERYKKGPVLIAEISKKKKIPLKFLENI
                     LLELKKADILDSKKGKGGGYFLKKDPAKVKMATVIRLVNGPIAMLPCVSLYFYERCKN
                     CDEQHCGLHDMMVEVRDATLSILENRTLQDLVV"
     gene            complement(2541..>2815)
                     /locus_tag="PK977_10025"
     CDS             complement(2541..>2815)
                     /locus_tag="PK977_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRF18498.1"
                     /translation="GLAGCSGIDVVDILEKMRVKFSHFEIETEATQTDDHPKVFRDVL
                     ITYRINTDKENEEKVKKAIDLSLEKYCGVSAMLRKNSPVNYKLVIE"
     assembly_gap    2816..2828
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2940..3905)
                     /locus_tag="PK977_10030"
     CDS             complement(2940..3905)
                     /locus_tag="PK977_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRF18499.1"
                     /translation="MNIQKWVAAVSVILLSVKFLAYYSTHSVSILTDALESIVNVAAG
                     LIGLYSLYVAAKPRDKNHPYGHGKAEFLSAAVEGTLIGSAGAIILYKAIQTLINPVEL
                     QKIDYGIWLIAATALVNYIMGYFCLRTGNRNSSLALIASGRHLQSDTLSTVGIIAGLI
                     LLYFTKLIWIDSVVAILFGVIIMITGYRILRKSIAGIMDEADTVLLDRFVGILNANRK
                     ENWIDLHNLRVIKYGSILHVDCHLTVPWYLNVHEAHKEIDALAELVRKEFGESLELFV
                     HSDGCLPFSCKICSKAGCPERKQEFEKRINWTLENISNNKKHELA"
     gene            complement(3937..4713)
                     /locus_tag="PK977_10035"
     CDS             complement(3937..4713)
                     /locus_tag="PK977_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18500.1"
                     /translation="MRMLFVSFVLAALISCNSSGEKDKDPLVTDTTKTDKTGQQVAPI
                     EPVKAAASDVPATIKITGNIHEVWKWNDKMGDNLLITSYVAPYEDKNKNEYGEEGFTG
                     ELYATHYAGTNGKFQQIWYISDAEKSCPFDVTCQFIRDAISITDLDKDEVAEITVQYK
                     LACRSDVSPAIMKLAMYEAGTKYGLKGLMWLAASPEDKFAVTENNANLEKLPGYKGTE
                     EEYMKTFGRYESEKEFSQAPPEFIMHARGRWMRFVKESFD"
     gene            complement(4806..>6184)
                     /locus_tag="PK977_10040"
     CDS             complement(4806..>6184)
                     /locus_tag="PK977_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18501.1"
                     /translation="GTIRMSQNGASGTADWVDNTVTGYNYGTGKVIFNGNGGHLINSK
                     STFGQIDIDASGHATLGSDVQANKWYLKNGRLNTTGSYKAIVLSSDQLAVEADPSNTN
                     FSNSWVNGNLRRYVLPASVSTYIFPVGDAVKANRAVLDNLIASPLNNLTYIDASFGPK
                     PGNDIGLAVTENGQPYISVNTGGVWYLTPDAAPTSGTYDLLLNFNGFTALADNSFAIL
                     KRPNSSSSAADWQSPAGSVLPANNQPGRILSAGYARRNNINSFSQFGIGQLSGALPVT
                     LVDFDAKRLTRQKVQVNWQTVFEQNNLGFDVERRLDGESVFTTIGFAPTKALNGNSTS
                     QIDYSYTDANGYSGISYYRLKQIDRDTRYTYTHIKAVKGMGETQVSVLLFPNPNYGQF
                     SIRLDGVNRSFDAYITDMSGKVVKQLRLTNNNSINISGLTSGTYLIKIPDVFGAGESF
                     TEKVLVIK"
     assembly_gap    6185..6219
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6592..7008)
                     /locus_tag="PK977_10045"
     CDS             complement(6592..7008)
                     /locus_tag="PK977_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF18502.1"
                     /translation="MAASVRLSTESLINTANDKDRTLRRHKIEWHRKIVLSLACLVLF
                     LVGAPLGSIIRKGGLGTPLIFAIIFFMVFYFSSTTGEKFAKENTFTPFTGMWMATFVL
                     TPVGIFLTYKAMRDSQLFNKEFYYRAARFIKGLFSR"
     gene            complement(7074..>7325)
                     /locus_tag="PK977_10050"
     CDS             complement(7074..>7325)
                     /locus_tag="PK977_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18503.1"
                     /translation="GFKEYKKQFDLSTLGFTNRTADSVNKNNEKMLSMRQLQKAIDSL
                     QKENRRIKDQMTKDMLLQFHFSSRPDSFWLQPALNLPEK"
     assembly_gap    7326..7463
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7464..>7958)
                     /locus_tag="PK977_10055"
     CDS             complement(<7464..>7958)
                     /locus_tag="PK977_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF18504.1"
                     /translation="LVLIMQFFWLYIDDFVSKGLGTGIILEFILYLSATLVPLALPLA
                     ILLSSLMTFGNLGESFELVAIKSAGISLLRFMRPLFFVTILISGIAFVFANNVIPIAN
                     LKQRTLLSDIVFAKPAFDLKEGVFYDKIPGFAIKIGKKEENDSVVRDVIIYEQGNPVQ
                     DNFIV"
     assembly_gap    7959..8014
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8056..8667
                     /locus_tag="PK977_10060"
     CDS             8056..8667
                     /locus_tag="PK977_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18505.1"
                     /translation="MNQAKIRLSSTEIELIKNADWILTKNAIIEKIVQFFSSLQEEQK
                     NELQLYKNKLPSRFFTASPKISKGENYSGLPYVVLDYPRFFNESGFGAIRTMFWWGNF
                     FSITLHMAGGNKKAFEEKLINAYPLLWQEKFYSCISENEWIHHFSPENYISFSEMTEA
                     EYRKRIIESQFVKIGTKINLDRWETAEDFLLSRFKTLLEILAG"
     gene            complement(8664..>8813)
                     /locus_tag="PK977_10065"
     CDS             complement(8664..>8813)
                     /locus_tag="PK977_10065"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRF18506.1"
                     /translation="EYFEFRIDKSEITNQTILVVKDFAEKKDIKDQSMLWDYQVKELF
                     HRLGN"
BASE COUNT         2572 a         1822 c         1625 g         2552 t
ORIGIN      
        1 aaaggtcaat ggtgaattag tgacagaacc cggccataaa gtttccacaa aagatgatat
       61 cagggttaat ggaaaaaaaa tctttctggc aaaaaacctt gtttacattc tcctcaataa
      121 acctaaagat tatattacca caacggatga tccccaggga aggaaaaccg tactcgacat
      181 cattggcagg gctaccaacg aaagagttta cccggtaggc agacttgaca gaaacacttc
      241 aggtgtttta ctgctgacaa acgacgggga actggctcaa agactaactc atcccagcaa
      301 tgaagtgaag aaagtatatc atgttacgct gaataagcca ctcgaaaaga aagattttga
      361 tcaaatattg aatggtgttg tattagaaga cggactggct aacgtagata cactggctta
      421 tgctgattca aaagataaaa cacaaattgg tgtggagata catagtggcc gaaaccggat
      481 tgtccgccgc atttttgaac atttgggtta tgacgtaaaa ggactcgaca gggttatgtt
      541 tgcgggttta accaagaaaa atgtagaccg gggtaaatgg agatttttaa ctgaaaagga
      601 agtaagggat ttaaaacact ttaaaaaagg aaagtaactt ctgactacac tgattactat
      661 gatttcgatg aaaacaacca attgggtaat tgaagaaact gagaattggg tagctgtaaa
      721 taagccttca ggtcttttat ccattccaga cagagaaggg aaagaagttt cattaaaagc
      781 aattctaaaa gaaaaatatg gtgacatttt tactgtccac cgtcttgaca aagataccag
      841 cggcctcata ctatttgcta aaaatgaaga agcacataaa cacttatcaa agcaatttga
      901 ggaaaggcgg accaaaaaaa tctaccaggg attagtaatc ggatgccctg aaaaacagca
      961 aggcagtatt gatagcccga tagcagagca ccccgcccaa aatggcatta tgatcataca
     1021 ccgaaaagga aaagaatcat taacagacta tgaagtactg gaagactttg gaatttattc
     1081 tttcctgcaa ttcagaatac ataccggacg cacccaccag atccgggtac atatgaaaga
     1141 tattggtcat cctattgttt gcgacaatct ttatggtgac ggaaaaccaa tcctggtttc
     1201 atcgttaaaa tcaaagttta aattatcaaa aaacgaagaa gaagaaaggc ccattttaaa
     1261 cagggtggcg ctgcatgcct accaattatc tcttattgat attaatggga gcctggttga
     1321 actggaggcc cctttacata aggacatgca ggcttcatta caacagctgc ataaaagaaa
     1381 aaagggcaag taattattat tgcccttaaa attactataa tgtaacaaag gttatttcag
     1441 atcaggctgc ggtgtatatc gcaaatacgg ttttatcact ttaattcctt tgggaaattt
     1501 tttaacagca tcctctgtag atattgcagg aactacaata acatcttctc cgcttttcca
     1561 gtctgccggt gtggccacac tgtatcctgc tgttaactgc aaggagtcga caacccgcaa
     1621 tacctcataa aaatttcttc ccgttgaagc cggataggta atcatcaatt tcaccttttt
     1681 atcaggccca ataacgaaaa gtgaacgtac cgtaaacgtt tcagaagcat taggatgaat
     1741 catatcgtaa agggtagcca cattcctgtc atcatcggca ataatcggaa atgtaacatt
     1801 acaatgctga gtttcattaa tatcctttac ccaaccctgg tgtttctcca gtggatcgac
     1861 acttactgca agcactttta cattccgctt tgcaaattct tccgcaagca atgcggtttt
     1921 acctaactct gttgtacaca ctggtgtaaa gtcggcagga tgtgaaaaca aaattcccca
     1981 accatctccg agatattcgt aaaaatcaat ttccccggct gtagtttttg ctttaaagtt
     2041 gggagcaata tcccctaaac gtagtcccat aaaaatggtt taggaaacaa aaataatata
     2101 ttcctaccag attagtagac ttatacaacc aggtcctgaa gcgttctgtt ttcaaggatg
     2161 ctgagggtag catcccttac ttctaccatc atatcatgaa ggccgcagtg ttgctcatcg
     2221 cagttcttgc atcgctcata aaaatagagg ctaacacatg ggagcatggc aatagggccg
     2281 tttacaagcc ggatgacagt tgccatttta acttttgcag gatccttctt gagaaaataa
     2341 ccgcctccct tacccttttt actgtccagg atatctgctt tcttcaactc aagaagaata
     2401 ttctccaaaa attttaatgg aatttttttc ttttttgaaa tctctgcaat caaaactggt
     2461 ccctttttat accgttcagt caggtaggtc aatgccttaa aagcatattg cgattttttg
     2521 gagagcattt caacaatttt ttattcaatt accaatttat aatttactgg tgagtttttg
     2581 cgaagcattg ctgatacacc gcaatacttt tccagcgaca agtcaatagc ttttttcacc
     2641 ttttcttcat tttctttatc cgtatttatc ctataggtaa tgaggacatc cctgaaaact
     2701 ttggggtgat catcggtttg tgtggcctcc gtttctattt caaaatgtga aaattttacc
     2761 cgcatctttt ctaagatatc cactacatct attcctgagc aaccggcgag cccggnnnnn
     2821 nnnnnnnngc aagagtgcct tagggctaaa tccatgctcc ttgcttccat ccaatttgat
     2881 gtaattatct ccttgccggc tttcaaattc atgctttttt ttccaaatgg tgatcgtttt
     2941 catgccagtt catgtttttt attattggaa atattttcaa gtgtccagtt aatccttttc
     3001 tcaaactctt gctttctttc cgggcaacct gccttgctgc aaattttgca ggaaaaaggg
     3061 agacaaccat ctgaatgaac gaaaagttcc aatgattccc caaattcctt tcttaccagt
     3121 tcagccagcg catcaatttc tttgtgggct tcatgcacat tcagatacca gggcacggta
     3181 agatgacagt ctacatgtaa tatactaccg tatttaatta ctcttaaatt gtgcaggtca
     3241 atccagtttt cttttcggtt cgcattcaat atccctacaa acctgtccag caaaaccgta
     3301 tcagcctcat ccattatacc agcaatcgat tttcgaagta tccgataccc ggttatcata
     3361 attataacac cgaacaaaat tgccactaca ctatcgatcc atataagttt agtaaaatat
     3421 agtaagatca agccggctat aatgcccaca gttgataagg tatcgctttg taaatgcctg
     3481 ccactggcaa tcagagccag tgaactgttt ctattacccg ttcttaagca gaagtatccc
     3541 attatataat tgaccagtgc agttgctgca atcagccaaa taccgtaatc tattttttgt
     3601 agctcaaccg ggttgatcag ggtttgaatt gccttgtata atataatggc ccctgcgctg
     3661 ccaatcagtg taccttctac cgcagcagaa agaaattcag ctttgccatg tccgtacgga
     3721 tggtttttat cccggggttt tgcagctaca tataagctat acaggccaat caggccagct
     3781 gcaacattca ctatgctctc caatgcatca gtaagaatcg atacagagtg agtggaatag
     3841 taggcaagaa actttacgga aagcaatatt accgatacag ctgccaccca cttctgtata
     3901 ttcaaatttt gttttgatct gtccaagtga tttcatttaa tcaaaagatt cctttacgaa
     3961 ccgcatccac ctgcccctcg catgcataat aaactcagga ggtgcttgtg aaaattcttt
     4021 ctccgattca tagcggccaa aagttttcat atattcttct tctgttcctt tgtaaccagg
     4081 cagcttttcg agattagcat tattttctgt aacagcaaac ttatcctccg ggcttgcggc
     4141 taaccacatc aaccctttca ggccatattt tgtaccagct tcatacatgg ccagtttcat
     4201 gattgccggt gacacatcac ttcggcatgc cagtttatac tgaaccgtta tttctgcaac
     4261 ctcatcttta tcaagatctg taatactaat agcgtccctg ataaactgac atgtcacatc
     4321 aaagggacat gatttttctg catctgagat ataccaaatt tgctgaaact taccattcgt
     4381 tccggcatag tgtgtggcat aaagctcgcc tgtaaatcct tcttcaccat actcgttctt
     4441 gtttttatcc tcatacggag ctacataaga agtaataagc aggttatctc ccattttatc
     4501 attccatttc cacacttcat gaatgttgcc ggtaattttt atcgttgcag gaacgtctga
     4561 tgctgctgct tttactggtt caattggtgc aacttgttgt cctgttttat ctgttttggt
     4621 ggtatcagtt accaatggat ccttatcttt ttctccggat gaattgcagc taattaatgc
     4681 agccagtacg aaagaaacaa ataacatccg catagtaatt tgttttggtc tgccgaaatt
     4741 actgaaataa aattgccctt gctgtaaaca caacaaaggc aatccaggtt aaggttggtt
     4801 aaaggttatt tgatcactag aaccttttcg gtaaaagact caccggctcc aaatacatcg
     4861 gggatcttta tgagatatgt tcctgaagta agtccggata tatttatact gttattattg
     4921 gttagcctta attgttttac aactttaccg ctcatatctg ttatgtacgc atcaaacgat
     4981 ctgttgacgc catccagcct gatagaaaac tgtccgtaat tgggattggg aaacaacagc
     5041 acactaacct gtgtttcacc catgcctttt acagctttga tatgagtata agtgtaccgg
     5101 gtatccctgt ctatttgctt aaggcgataa taactgattc cggaatagcc gttggcatct
     5161 gtataggagt aatcaatttg tgatgtactg ttgccattta atgcttttgt tggagcaaag
     5221 ccgatagtag taaatacgga ttctccatcc agccttcttt ccacatcaaa tccaagattg
     5281 ttttgctcaa aaactgtttg ccagttcacc tgtacttttt ggcgggtcag cctttttgca
     5341 tcaaaatcta ccagtgtaac aggcaaggct cctgaaagct ggccaattcc aaactgactg
     5401 aaactattaa tgttgttcct cctggcatat cctgcggaaa gaattcgtcc tggttggtta
     5461 ttcgcaggca atacacttcc ggcaggagat tgccaatctg ccgcactgct tgatgaatta
     5521 ggtctcttaa gaatagcaaa tgaattgtct gcaagagctg taaacccgtt aaaattaagc
     5581 aacagatcat aagttccgga ggttggtgcc gcatctggtg ttaaatacca aacaccacca
     5641 gtattaactg aaatataagg ctgcccgttt tctgttactg ctaatccaat atcattaccc
     5701 ggcttaggtc caaatgatgc atctatataa gtaagattat ttaacggaga agcgattaag
     5761 ttgtctaaga cagccctgtt cgcttttact gcatcaccaa ctggaaatat ataagtactt
     5821 actgatgcag gcaatacgta ccttctaagg tttccattta cccaactatt ggaaaaattg
     5881 gtgttggaag gatcagcctc tactgccaat tgatctgaac taagaacgat ggccttataa
     5941 gaccctgttg tatttagcct cccatttttc aggtaccact tattggcctg cacatcactg
     6001 cccaatgtag catggccaga agcatcaata tctatctgtc caaaagtgct tttggagttg
     6061 atcaaatgac caccattccc gttgaaaatg actttacctg ttccatagtt atacccggta
     6121 accgtgttat ctacccaatc tgctgtgccg gatgccccgt tttgagacat acggatggtg
     6181 cctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aaactccccc ttttactaca
     6241 agctctgctc cattttgcac cgtaataacg gcacctgatt gcaccttcat aacatcctgc
     6301 gccaacatta cttttcctgt aaaaaacaag gacagaatta gtaaagattg ctttctcatg
     6361 gttgcagcaa gatttttggt tgattgcata atctttattt ttcggttcaa tggttttttc
     6421 aatgattccc tgaaattccg acattgccgg caggttttca ttacggaatt agtaaacgga
     6481 agtcttgatt gagtaagcgg atatatgctg gactaaaatt gaagaatttt cggttaccat
     6541 cctataccac aaacaggtga ttttttggat aaaactggcc aaaaaaggga ctcagcggct
     6601 aaaaagccct ttaatgaacc gggcagcccg gtagtagaac tctttattga ataactggga
     6661 atcccgcatt gccttatagg ttaaaaagat acctacaggg gtaaggacaa aagtagccat
     6721 ccacatacct gtgaagggag taaaggtatt ttccttggca aacttttcac cggtggtact
     6781 tgaaaaataa aaaaccataa agaaaatgat ggcaaaaatc aaaggtgtcc ccagtcctcc
     6841 ttttcggata atggacccca ggggagcacc caccaggaat aaaaccaggc aagcgaggga
     6901 aagaacgatt ttacgatgcc attcaatttt atgcctgcgc aatgtcctgt ccttatcgtt
     6961 tgccgtgttg ataagggatt cagtgctaag cctgactgag gctgccatat tttgcacatt
     7021 ctggtttacg ttaccttccg ctgaatccgg taacaagaga tcaaaccgct ttactacttc
     7081 tccggcagat tcaatgccgg ctgaagccaa aaagaatcgg ggcggctgga gaaatgaaac
     7141 tggagcaaca tgtcttttgt catctggtcc ttgatgcgcc ggttttcttt ctgcaaggag
     7201 tcaattgctt tttgcagctg ccgcatgctg agcattttct cattgttttt gttgacgctg
     7261 tcagccgtac ggttcgtaaa acccagcgta cttagatcaa attgtttctt atattcttta
     7321 aacccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnacgataa aattatcctg caccggattg ccttgctcgt
     7501 agatgatcac atccctgacc acactgtcat tttcttcttt ctttccaatc ttaatagcga
     7561 acccgggtat cttatcatag aatactcctt ctttaaggtc aaaagctggt ttggcaaaaa
     7621 caatatccga cagtaaggtc ctctgcttca ggttagcgat cggaattaca ttgttggcga
     7681 aaacaaaggc aattcctgaa attagaatcg ttacaaaaaa aagcggccgc ataaaccgca
     7741 ggagtgagat ccctgccgat ttgatagcaa ccagttcaaa actttctcca agattgccaa
     7801 aagtcatcag tgatgaaagt aagatggcca gtggtaaagc cagcggaacc agtgttgcac
     7861 tgaggtataa tatgaactca agaatgatac ccgtacccag tcctttgctt acaaaatcat
     7921 caatatacaa ccagaaaaac tgcatgataa gtaccagcnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgatg agcttatcta gttttttgaa
     8041 cacttatctt taagtatgaa tcaggcaaaa ataaggcttt catcaaccga gatagaactg
     8101 ataaaaaatg ctgactggat tttaacaaag aatgccatta ttgaaaagat cgtacaattc
     8161 ttttcctcat tgcaagagga gcagaaaaat gaactgcagt tatataaaaa caaactccct
     8221 tcccggttct ttactgcctc acccaagata tctaaaggtg aaaattattc aggtcttccg
     8281 tatgtagtgc tggattatcc ccgttttttt aatgagtccg gtttcggagc aatcagaacg
     8341 atgttttggt ggggaaattt ctttagtatc accctgcata tggccggagg caataaaaaa
     8401 gcatttgagg aaaagctaat taatgcctat cctttgctat ggcaagaaaa attttacagc
     8461 tgtatcagtg agaatgaatg gatacatcat ttctcccctg aaaactatat ttctttttca
     8521 gaaatgacag aagctgaata caggaaacga ataatagaaa gtcaattcgt taaaatagga
     8581 acaaaaatta atttagacag atgggaaacc gctgaagact ttttgctcag ccgctttaaa
     8641 actctgctgg agatactggc tggttaattg cctaaacggt gaaagagttc cttaacctgg
     8701 taatcccaca acatactctg gtctttgata tcttttttct ctgcaaagtc ctttacaacc
     8761 agaattgttt gattggtgat ctcagattta tcaatgcgga attcaaaata ttc
//
LOCUS       DAORLS010000118         8789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4318_length_8789_cov_2.174262, whole genome shotgun sequence.
ACCESSION   DAORLS010000118 DAORLS010000000
VERSION     DAORLS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4318_length_8789_cov_2.174262"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..>775)
                     /locus_tag="PK977_10070"
     CDS             complement(161..>775)
                     /locus_tag="PK977_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF18507.1"
                     /translation="TSDKSPKDLEGVQERLLSRFRWGLSADLQVPEYETRIEILEKKM
                     KNDGLEMPKEVVKYIAYNISNNVRELEGALISLLAQSSLNRREIDLDLAKKVLRNFVK
                     SSSKEITIETIQKMVCEYFDVPYDKLLQKTRKREIVQARQITMYLAKAFTKNSLKTIG
                     EHFGGRDHTTVIHSCQTVKDLMDTDGVFRENVLELQQKVQLAAM"
     assembly_gap    776..794
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<795..>1577)
                     /locus_tag="PK977_10075"
     CDS             complement(<795..>1577)
                     /locus_tag="PK977_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRF18508.1"
                     /translation="TNCLKIIKDIVEWQHYKTWFEPIKPVSLKNNVLVIQVPSQFFFE
                     YLEEHYVNLLAKTLKRELGKEARLEYRIMVDSGNSKNKPVTMDVSGQGFKTFSNNEMD
                     FPLVINNPVKNPFVIPGLKKMQIDTQLNHIYTFESLIEGDCNRVARRAGKTVAEKPGA
                     NSFNPLVIYGGVGLGKTHLAQAIGNEVKRLHPNKVVLYVSSEKFINQFMDHSRNNAIN
                     DFIHFYQLIDVLIIDDVQFFAKAEKSQDAFFAIFNHLHQSHK"
     assembly_gap    1578..1652
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1738..>2641)
                     /locus_tag="PK977_10080"
     CDS             complement(1738..>2641)
                     /locus_tag="PK977_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRF18509.1"
                     /translation="AMSAGAIMAGPALTQTADYFSPLKLTILHTNDTHSRLEPFPMDG
                     GRNQGLGGIAGRMQLINQIRSEEEHVLVFDAGDIFQGTPYFNIYKGEPEIKAMSAMQY
                     DACTIGNHDFDAGMENLATQLTNHAKFPMLVCNYDFTGTPMEFKTQPYKVYRKGKLKI
                     GVFGVSIEGKGLIPDNLFGATKYLDPIQKANEVADKLRKQEDCDMIICLSHLGHQYKD
                     NKVSDEVLAKESENIDLIIGGHTHTFLDNPVKYTNKKGDDVIINQVGWAGIILGRLDF
                     EFSKFSGKKLTKNHSITVSQKSGG"
     assembly_gap    2642..2796
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2797..3425)
                     /locus_tag="PK977_10085"
     CDS             complement(<2797..3425)
                     /locus_tag="PK977_10085"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRF18510.1"
                     /translation="MRKNPLMALAGLLVFIASLSCNTTYQSQSLQYKSYRITDTISKN
                     TAALSLLQPYSDNVNKKMNEVVGIAEISLEKKQPECTLGNFLSDAFLVMAQEKYNTRA
                     DVALINFGGMRLTQLPKGEVTNGKIFELMPFDNLLILQKVRGDILQQFLDLIAAKGGW
                     PVAGMTMQIKDKKAINVMIGGKPLDPKATYTIVNSDFIANGGDNADMLRP"
     gene            3547..5109
                     /locus_tag="PK977_10090"
     CDS             3547..5109
                     /locus_tag="PK977_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF18511.1"
                     /translation="MQKNLTLKLLPSEAASDSIIKNYIASTEVVNPSSVTGFIINKHS
                     IDARGKQVWINLSITAFINEPFRQRELVSFTFQQVEKAEKKVIIIGAGPAGLFAALQL
                     IEKGIKPIILERGKDVRARRRDLAILNKQGEVNPESNYCFGEGGAGTYSDGKLYTRSS
                     KRGDINRILNLFVHFGAEEKILFESHPHIGTNKLPHIITAMRHKIIECGGEFLFEKKV
                     VDFTITNEEITRVRTGDGDLFQADAFILATGHSARDIFELLNNKKIWIETKPFALGVR
                     VEHPQSLIDSAQYHCAVRSEYLPPAAYSWVQQVNGKGVFSFCMCPGGIIAPAATSPGE
                     LVVNGWSPSKRNNSFANSGIVVSIEAKDIKAFEKFGPLAAMYFQQSVEKKAFEKGGGK
                     FVAPAQRLVDFTENKISSSLPDCSYLPGISSVSLKEVLPGFIHQRLQQGFKEYGKKMK
                     GYLTNDAIVVATESRTSSPVRIPRNPDTLQHPQISNLYPGGEGAGYAGGIVSAAMDGE
                     RIATVLSNKLTN"
     gene            5214..6149
                     /locus_tag="PK977_10095"
     CDS             5214..6149
                     /locus_tag="PK977_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF18512.1"
                     /translation="MPILELQNLKKYFATQKAVDDISLNVEKGQIFGLLGPNGAGKTT
                     LIRMITGIFYPDEGNIIFDGKKFDPINDVAYIGYMPEERGLYKKMKIGEQAMYLAQLK
                     GLSHKEAMEKIKKWFIRFEMETWWNKKVEDLSKGMGQKLQFVTTVLHEPKLIILDEPF
                     SGLDPVNSNLIKDEIFHLAQAGSTIIFSTHRMEQVEEICDHIVLVNKGKKILDGTVKQ
                     VKQDFKENLFSIGTDQTTISADANAPFEIVGAKDHSYVVRIKSGNSPNDVLRHLLQQN
                     ISIHSFNEILPSLNDIFIKLVEGTPTARQFQKVTA"
     gene            6174..>7338
                     /locus_tag="PK977_10100"
     CDS             6174..>7338
                     /locus_tag="PK977_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18513.1"
                     /translation="MKKTWIIIKREYLTRVRKKTFIISTLLFPLLYILLIFGMGYIAK
                     ESVQNLKVAIIDSSGYFTAEKLAFQNSIDKSSTLLLVKEEAQKVIADHQAEGYDGYII
                     IPAMNWQTGTDSLFFKANKSYGSSSTTLVESKLNRIWNEIKNDSLQIDGEKQKILAGS
                     HLGLSSKNIKDERANVKIAEGIGYVSGLLIYFILLIYGSQVMMGVMEEKTNRIAEVLV
                     SSVKPFQMMLGKILGIGLVALTQFFLWIAFVLIVYNIGKATGGTDGITGQVVGNMQNL
                     FSSVDVPLILFCFGFYFLAGFFFYSSLYGAIGSAVNEDMREAQSLSFPITMLVILSIA
                     MMSAAIANPTSPLAVWASIIPFSSPIVMMARIPFGVPNTVPWWQLGLSMALLIL"
     assembly_gap    7339..7359
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7625..8461
                     /locus_tag="PK977_10105"
     CDS             7625..8461
                     /locus_tag="PK977_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18514.1"
                     /translation="MKPTNFMNLRLLFAVAAIGLTIGLVSWDRQQSPGKYEQSINDTT
                     PKKVKDREKKVRDLDDVLDELNEAELDIDMNKIRQEINESLKNVNAEKIKQDIEKAFK
                     EIDLDKIKAEVEASLSKVDFDNIKKQMQEAMKQLDEVKIQKEIQESMAKVDWEKIKAE
                     MEKVKDVDMKNLEKEMKKVQEEMKDLGPKLEKEMAKAKEGLEKAKAEVKEFKTFVDGL
                     EKDGLISKKEGYILKHKDGELLINGKKASEQTYNKYRSFLEKHKKFNIEKTDDDFDMN
                     LD"
     gene            complement(8540..>8789)
                     /locus_tag="PK977_10110"
     CDS             complement(8540..>8789)
                     /locus_tag="PK977_10110"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF18515.1"
                     /translation="TITAYLVSKKEKISATNDIGEFSFDELKPGTYKFVFEKAGFRKV
                     TKEKVVVKTDEAFQMNIEMIEAGDYDIMPSPFNIGGGI"
BASE COUNT         2603 a         1732 c         1725 g         2459 t
ORIGIN      
        1 aagcccgtac aacggtttag caaaccggcc ctttcggcca ctcaggcacc tctccatttg
       61 ggggaagcaa aaataaagat taatgctttc aaatcccaaa aaaaacaaat cccaaattcc
      121 ttatatgctg gaatttggga tttgatactt gaatatttat ttacatggca gccagttgca
      181 ctttttgctg taattccagc acattctctc tgaaaacacc atcagtatcc atcaggtctt
      241 ttaccgtttg acaggaatgg atgacagttg tatgatcccg gccaccaaaa tgttctccga
      301 tggtcttcag tgaatttttg gtaaatgcct tggccaggta catggttatc tggcgggcct
      361 gtactatctc tcttttacgg gttttctgta agagtttatc gtacggcaca tcgaaatatt
      421 cgcaaaccat tttttggatg gtttcgatcg taatttcctt actggatgat ttgacaaaat
      481 tgcgtaacac tttctttgcg aggtctagat caatttccct cctgttaagc gaagattgtg
      541 ccaatagcga tatcagggcc ccttctaatt cccttacgtt attactaata ttataagcaa
      601 tatacttaac tacctcttta ggcatttcga gcccgtcgtt cttcatcttt ttctccagaa
      661 tttctatgcg ggtctcgtat tccggcactt gcaggtcggc actgagcccc cagcggaaac
      721 ggctgagtaa tctttcctga acaccttcca ggtctttggg tgatttgtcg gaagtnnnnn
      781 nnnnnnnnnn nnnngcttat gtgactgatg caggtgattg aaaatagcga agaatgcgtc
      841 ctgcgatttt tcagcctttg caaagaattg tacatcatcg atgatcaata catcgatcag
      901 ctggtaaaaa tggataaagt cattaatagc attattccgg ctatgatcca tgaactgatt
      961 gatgaacttt tcggaactca catacaacac tactttattt ggatgaagcc tttttacttc
     1021 gtttccgata gcctgtgcca ggtgtgtttt tcccaaacct acgccgccgt atattaccaa
     1081 cgggttgaat gagttagcac cgggtttttc tgctactgtt ttaccggccc tgcgggctac
     1141 ccggttgcaa tctccttcta ttaaagattc aaaggtatag atatgattaa gctgggtatc
     1201 aatctgcatt tttttgagcc cagggataac gaagggattc ttaacaggat tatttatgac
     1261 taaaggaaaa tccatttcgt tatttgaaaa agttttaaaa ccctgaccgc ttacatccat
     1321 ggtaacaggt ttgttcttac tgttcccgct atcaaccatg atgcgatatt caagcctcgc
     1381 ctccttaccc aattcccgct tcaatgtctt cgctaataaa tttacgtaat gttcttcgag
     1441 atattcgaaa aagaattggc tgggaacctg gatgacgaga acattatttt ttagtgatac
     1501 aggtttgatg ggctcaaacc aggttttgta atgctgccat tcaacaatgt ctttgatgat
     1561 ctttaaacaa ttggtccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtccaaaa aaaatcttca cactatgtca
     1681 ataaaaaaag ttggacagga cggcgaatat caccatttct tttctaaaaa aaaattttta
     1741 cccacctgat ttttgtgaaa cagtaatgga atgatttttt gtcaattttt tacctgaaaa
     1801 tttggaaaat tcgaaatcaa gtctacctaa aatgatgcct gcccagccta cctgatttat
     1861 tattacatca tctccctttt tatttgtata cttgacgggg ttgtccaaaa aagtatgtgt
     1921 atgccctccg atgatgagat caatattctc tgattctttt gccagtactt catcgcttac
     1981 tttattgtct ttgtattgat ggcccaggtg agataaacag atgatcatat cacaatcttc
     2041 ctgtttcctc agtttatccg ctacttcatt tgccttttgt ataggatcga ggtactttgt
     2101 agccccaaac aagttatcag gtattaaccc ctttccctcg atgcttacgc caaatacccc
     2161 gattttcaac tttcctttcc tgtatacctt gtatggttga gttttaaact ccattggcgt
     2221 accggtaaaa tcataattac aaaccagcat cggaaatttc gcatggttgg tcagttgcgt
     2281 ggcaagattc tccataccgg catcaaaatc atgattaccg atcgtacagg catcatattg
     2341 catcgccgac attgccttta tttccggttc acccttataa atattgaaat aaggcgtgcc
     2401 ctggaaaata tcgccggcat caaaaaccaa tacatgttct tcttcactcc ggatttggtt
     2461 gatcagttgc atacgtcctg caattccacc taatccctgg ttgcgtccgc catccatagg
     2521 aaaaggctcc agccggctat gtgtatcgtt ggtatgcaga atggtcaatt tcaaagggct
     2581 gaaataatct gccgtctgcg tcaaagcagg tccggccatt atggcacctg ccgacatagc
     2641 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtc ttagcatatc cgcattatct
     2821 cccccattcg caataaaatc agaatttaca atagtatagg tagcctttgg atcaagaggt
     2881 tttccaccaa tcattacatt gatggctttt ttgtctttga tttgcatcgt cataccagct
     2941 accggccagc ctcccttggc agctataagg tccaaaaact gttgtagtat atctcccctg
     3001 accttttgta aaataaggag attgtcaaag ggcatcaatt caaatatctt tccattggta
     3061 acttctccct ttggcaattg ggtcaaccgc atacccccga agttgatcag tgctacatcg
     3121 gccctggtat tgtatttttc ctgtgccatc actaaaaatg catctgataa aaaattgccc
     3181 aatgtgcatt ccggctgttt tttttccagg ctgatctccg caatgcctac cacttcattc
     3241 atttttttat ttacattatc actgtaaggt tgtaacaatg ataatgcagc agtattcttt
     3301 gaaatggtat cagtgattcg atatgacttg tactgtaagg attggctttg gtaagtggta
     3361 ttgcaggata aacttgcaat aaaaactaaa aggccggcta gtgccattaa cggattcttt
     3421 ctcatataaa gcagtctgat ttgaaggtat acaattttca aatgcaacaa aaaatcctga
     3481 gccatttgaa gccaaatttt ctttgcatct ttgttttata tttttttcag gataaatacc
     3541 tgaccaatgc aaaaaaacct tacattaaag ctgcttccct ccgaagctgc cagcgactca
     3601 attataaaaa attatatcgc cagcacagaa gtggttaacc catcttcggt cacaggtttt
     3661 attatcaaca aacactccat tgatgccagg ggaaagcaag tatggattaa tctttcaatt
     3721 acagctttta tcaatgagcc attccgccaa agggagctgg tttcatttac ctttcagcag
     3781 gtagaaaagg cagagaaaaa agttataatt attggtgccg ggcctgcagg cttgtttgcc
     3841 gcattgcaat tgattgaaaa aggtattaaa ccaataatac tggaacgggg taaagatgta
     3901 agggccagaa gaagagacct ggccatactt aataagcaag gagaggtaaa cccggaaagt
     3961 aattattgct ttggtgaagg aggcgcaggc acttacagtg atggtaaact atatacccgc
     4021 agcagcaaac ggggagatat taacagaatc ctgaaccttt ttgtccattt tggagctgaa
     4081 gaaaaaatat tatttgaatc acatccgcat attggtacta ataaactgcc tcacatcata
     4141 acagctatgc gccacaagat catagaatgt ggcggtgagt ttctctttga gaaaaaagtg
     4201 gttgacttca ctattactaa tgaagaaatt acccgagtaa gaacaggaga tggcgattta
     4261 tttcaagctg atgcttttat attggctacc ggccattcag cccgggatat ttttgaattg
     4321 ttgaacaata agaaaatttg gatcgaaaca aaaccttttg cattaggggt acgggtagag
     4381 catccgcaat cactgattga cagtgcccaa tatcattgcg ctgtcagaag tgaatacctc
     4441 cctcctgctg cctactcctg ggtgcagcag gtaaacggaa agggtgtttt ttctttttgt
     4501 atgtgccctg gcggtatcat agcgccggca gccacatctc ccggtgaatt ggtagtaaat
     4561 ggctggagcc cttccaaacg caataattca ttcgccaaca gcgggatagt ggtgtccata
     4621 gaagcaaaag atattaaggc ttttgaaaaa ttcggtccgt tggcagccat gtattttcag
     4681 caatcagttg aaaaaaaggc ttttgaaaaa ggaggaggaa agttcgtagc cccggcacaa
     4741 cgcctggttg attttacaga aaataaaata tcctcttcct tacccgattg ttcgtatttg
     4801 cccggtatca gctctgtttc gttaaaggaa gtactacccg gatttattca ccaacgactt
     4861 caacagggat ttaaagaata tgggaaaaaa atgaagggtt atttaacgaa tgatgccatt
     4921 gttgtggcta cagagtcaag aacatcttcc ccggttcgga taccccgtaa tcccgacacc
     4981 cttcagcatc cccagataag caacctttat cctggtggtg agggtgccgg ctatgccggt
     5041 ggtatcgtaa gcgccgccat ggacggagaa agaattgcca cagtactttc aaataaatta
     5101 acaaactgat gaccacaggc cgcagagtga ttttctcact actaataacc actcaccaaa
     5161 tttcccggcc tgtaaagtaa tcaggagtaa ttttgcaccc acaaaccata actatgccta
     5221 tactcgaact ccagaacctg aaaaaatact tcgctaccca aaaagctgta gatgacataa
     5281 gccttaatgt agaaaaggga caaatttttg gtttgctggg tcccaatgga gccggcaaaa
     5341 caacccttat ccgcatgatt accggaatat tctacccgga tgaaggaaat atcattttcg
     5401 acggaaagaa gtttgaccct ataaatgatg tggcttatat cggttatatg ccggaagaaa
     5461 gaggcttata caagaaaatg aaaattggcg aacaggccat gtaccttgcc cagctcaaag
     5521 gcctcagtca taaggaagcc atggaaaaga tcaagaagtg gtttatccgt tttgaaatgg
     5581 aaacctggtg gaataaaaaa gtggaagacc tttcaaaagg gatggggcaa aaattgcagt
     5641 ttgtaacaac cgtattgcat gaacccaaat taatcatatt ggatgaacca ttcagcggac
     5701 ttgacccggt gaacagcaac ctgattaaag atgaaatctt tcatttggca caggctggtt
     5761 ctaccattat tttcagtacc caccgtatgg aacaagtgga agaaatatgc gaccatattg
     5821 tgctggtaaa taaagggaag aaaatattgg atggtaccgt taaacaagtc aagcaggact
     5881 ttaaagaaaa cctatttagc attggtacag atcaaactac gatatcagct gatgcaaatg
     5941 caccttttga aattgtaggg gctaaagatc attcatatgt cgttcggatt aaaagcggta
     6001 atagcccgaa tgatgtacta aggcacttgc tgcagcaaaa catttccatt cactcattca
     6061 atgaaatatt gccttccctt aacgacattt ttataaaact agtggaggga acaccaactg
     6121 cccgccagtt tcaaaaagta actgcataac cattacacca aaagattttc attatgaaaa
     6181 agacctggat catcataaaa agagaatacc tgacaagggt gagaaagaaa acattcatca
     6241 tttctacctt gcttttcccg ctgctttata ttctcctcat tttcggcatg ggatatattg
     6301 caaaagaaag tgtacaaaac ctgaaagtgg ccatcattga ctcatcagga tatttcactg
     6361 ctgaaaaact ggcttttcaa aattcaattg acaaaagttc tacgctgtta ctggtaaaag
     6421 aggaggcgca aaaagtgata gcagatcacc aggctgaagg atatgatggt tatattatca
     6481 tccccgccat gaattggcaa accggtactg acagcctttt tttcaaagcc aacaaatcat
     6541 atggcagcag ttctacaact ttggttgaat caaaacttaa ccggatttgg aatgaaataa
     6601 aaaatgacag ccttcagata gatggtgaaa aacaaaaaat tctggctggc agccatctcg
     6661 gactcagttc caagaatatc aaagatgaaa gagccaatgt aaaaattgct gagggtattg
     6721 gttatgtaag tggcttgctg atctatttca ttctgcttat ttatggttca caggtgatga
     6781 tgggtgtgat ggaagaaaag acaaaccgga ttgctgaagt actggtatca tctgtcaaac
     6841 cttttcagat gatgctggga aagattctgg gaatcggctt ggtagcctta acgcaattct
     6901 ttttgtggat tgcctttgtt ttaattgttt ataatattgg taaggctaca ggcgggacag
     6961 acggaatcac cggccaggta gtgggcaata tgcaaaacct tttctccagt gtggatgtgc
     7021 cgctgatatt gttctgcttt gggttttatt tcctggcagg cttctttttt tattcttcac
     7081 tttatggagc cattggtagc gcagtaaatg aagatatgcg ggaggcccag tcacttagtt
     7141 tccctatcac aatgctcgtc attctttcca tcgccatgat gagtgcggct attgccaatc
     7201 ccaccagccc cctcgctgta tgggcaagta ttattccctt tagctctcca attgttatga
     7261 tggcccgtat accttttgga gtacccaata cggttccctg gtggcaactg ggactttcga
     7321 tggcattatt gatacttgnn nnnnnnnnnn nnnnnnnnnt ttaccgtctg gtttgccggc
     7381 aagatatacc ggacggggat ccttatgtat ggcaaaaagc caacatggaa ggaaatgctc
     7441 cgttgggctt tccgaaaaaa ctaaactgga ttaacttttt tgaaaagccg ccctggttta
     7501 ccgggcggct tttttgttaa aatttccctt aaggataata gaaattagct aaatacgaaa
     7561 tcaatcgtat atttgatacg aaatatttcg tgaatcaaat taattagcca ttaaaaacaa
     7621 ttgtatgaag ccaacaaact ttatgaatct gcgccttttg tttgcagtag cggccattgg
     7681 tctaactatc gggctggtat cctgggaccg tcagcaatca ccggggaaat atgaacagtc
     7741 aataaatgat accaccccca aaaaagtaaa agacagggaa aagaaagttc gtgacctgga
     7801 tgatgtgctg gatgaactaa acgaagcaga actggatatt gatatgaaca agatccggca
     7861 ggaaataaac gaatctttga aaaatgtcaa tgccgagaaa ataaaacaag atatcgagaa
     7921 ggcatttaaa gagattgatc ttgataaaat aaaagctgaa gtagaagctt ctttatcgaa
     7981 agttgatttt gacaacatca aaaaacaaat gcaggaggcg atgaaacaac tggatgaggt
     8041 aaaaattcaa aaagaaatcc aggaatcgat ggcaaaagta gattgggaaa agataaaggc
     8101 cgaaatggaa aaggtgaaag atgtggacat gaaaaacctt gaaaaagaaa tgaagaaagt
     8161 gcaggaagaa atgaaagacc tgggacccaa actggaaaag gaaatggcaa aagccaagga
     8221 aggtcttgag aaggcaaaag cagaggttaa agaattcaaa acttttgttg atggtcttga
     8281 gaaagatgga ttgatcagca aaaaagaagg atatatccta aaacacaaag acggtgaatt
     8341 actgattaac ggaaagaaag catctgagca aacctacaac aagtaccgtt cgttcctgga
     8401 aaagcacaaa aagttcaata ttgaaaagac cgatgatgat ttcgatatga acctcgacta
     8461 gttatagccc aaaaccagcc atgaaaaatg aaaagggccg tcttaacaga cggcccttta
     8521 tcttaacata gatacttttt taaattcctc ctccaatgtt aaaaggagat ggcattatat
     8581 cataatcccc agcttctatc atctcgatat tcatctggaa tgcttcgtcg gttttcacca
     8641 ccaccttctc ttttgtaact ttcctgaaac cggctttttc aaacacaaac ttgtacgtac
     8701 ctggctttaa ttcatcgaaa gaaaattctc caatgtcatt ggtagcactg attttttctt
     8761 tttttgaaac caggtaagca gtaatggtt
//
LOCUS       DAORLS010000119         8723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4370_length_8723_cov_2.087217, whole genome shotgun sequence.
ACCESSION   DAORLS010000119 DAORLS010000000
VERSION     DAORLS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4370_length_8723_cov_2.087217"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>344)
                     /locus_tag="PK977_10115"
     CDS             complement(<1..>344)
                     /locus_tag="PK977_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18516.1"
                     /translation="LCVNTFAQDNKVDSVIVPVKEVPLIDTTIDYDALFRDFDAFMDS
                     ILSPNSYFLSSLSIGKGYYNFESKSAATITTTKKLTYSPMLGYYHKSGFGLTATGYLV
                     DDGQKMNLFQGG"
     assembly_gap    345..391
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(568..1839)
                     /locus_tag="PK977_10120"
     CDS             complement(568..1839)
                     /locus_tag="PK977_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF18517.1"
                     /translation="MRNTPFWWILIGFMALLDIYFFQALKVVAHSASPRTRTILYTSY
                     WVLSVAAIIVLMILPYIHFEKQARFARTTIFSIIAGLFFAKLIAAFFFVLDDLRRGVQ
                     WVAGKLFFQRTEGADLQSGENITRSAFLSWAGMMMGGTLFGTLVYGFGNKYRYQVKKV
                     KLSFDNLPSSFKGLRIVHISDIHSGSFTDKQAVMRGVEKILKEKPDLILFTGDLVNNV
                     AEEMNDYINVFDKLKAPLGVYSTLGNHDYGEYVNWDSEEDKKANLERLKQVHAAMGWR
                     LLMNEHVVLEKDGDKIALLGIENWSAKARFPKYGDLEKAYAGTENYPFKILMSHDPSH
                     WKEEVVPSYPDVNLVLSGHTHGMQFGVEIPGIKWSPVQYVYREWAGLYEKEANEGPTQ
                     AQKLYVNRGFGFIGYPGRVGILPEITVLELS"
     gene            1928..2608
                     /locus_tag="PK977_10125"
     CDS             1928..2608
                     /locus_tag="PK977_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRF18518.1"
                     /translation="MKYTYYGHSCFLVETNGKKLLFDPFISGNELAKNINIAGIEADY
                     ILISHGHVDHIADCVAIAQSTGAKVVCSWEIYEWLGKKGVTKAHPMNTGGQWHFEGFS
                     VRCVVAQHSSGLPDGSYGGNPMGFIISNEEGSFYYSGDTALTLDMQLIPRWAKLNFAV
                     LPIGDNFTMGYEDAVEAARMIGCNTIIGVHYDTFGFIKIDHTKATVTFEKAGCKLHLV
                     EIGKSITL"
     gene            2627..>3259
                     /locus_tag="PK977_10130"
     CDS             2627..>3259
                     /locus_tag="PK977_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF18519.1"
                     /translation="MGRIIGVANQKGGVGKTTTAINLAASFAVLEYKTLLVDGDPQAN
                     STTGVGFDLHNITQSLYDCMVNHAKAKDVILKTEIPNLDLIPSHIDLVGAEIEMINYP
                     NREMVLKNILEAIRNEYDFIIIDCSPSLGLITVNALTAADSVVIPVQAEFFALEGLGK
                     LLNTIKIVQSRLNTSLVIEGILMTMYDGRLRLCNQVVSEVRRHFDDMVFST"
     assembly_gap    3260..3326
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3495..4424
                     /locus_tag="PK977_10135"
     CDS             3495..4424
                     /locus_tag="PK977_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRF18520.1"
                     /translation="MTNPKQNKELLGKGIRSLLQSIDSDLKTSSGHLKSNVVENVTGI
                     NRIPIADIEANPKQPRRDFDEKALQETVYYFASNIGKMHDIIQPVTVSKLPSGKYRLI
                     SGERRWRASKLAGIQDIPAYVRQANDAQLLELALLENLQREDLNAMEIALSYKRMMEE
                     LDFTQEQVAERMGKDRSTVANFIRLLKLPPDIQLAVRNGELSMGHARALINVDTIDKQ
                     LYIFKEIKNKGLSVRQTEALVRNLYKDGAVKKTSKSQLHGPFQRIEDKLASHFSTRVK
                     LRHSRNGSGQITIDYYSTEELNKILRQMNAPLD"
     gene            4427..5056
                     /locus_tag="PK977_10140"
     CDS             4427..5056
                     /locus_tag="PK977_10140"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRF18521.1"
                     /translation="MRIASVIIFSLIILTSSCLYAQEKVPVAVSAKDSIPVTGKKKNI
                     GSRYDSLSKAHSPRKAAIRSALLPGLGQIYNKKYWKLPIVYGALGTCAGIFIYNLGNY
                     KDTKFAYRVKYNMRVFRTDSASFVNIKDNLKPLSEESLKFYRNQFRRDIDYSALVFIL
                     LWGLNVVDATVDAHLKSFDVSPDLSLRIKPGYSDLARTNGLSLILKIGK"
     gene            5148..5864
                     /gene="dapB"
                     /locus_tag="PK977_10145"
     CDS             5148..5864
                     /gene="dapB"
                     /locus_tag="PK977_10145"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRF18522.1"
                     /translation="MKFVLIGFGKMGKAIEEVALQRGHQVILKIDQPNLHEFTKEKIA
                     LADAAIEFTSPHSAYENVKKCIDFDIPVVCGSTGWTEKLNEMKKHATEKNVGFVYSSN
                     YSVGVNIFFEVNKKLAELMAPHKEYEVILEETHHTQKKDAPSGTAITLAEQILEKVKR
                     KKQWVNELSDNPEDLEIISQRVDPAPGTHSVKYSSVIDNIEIIHTAHSRTGFATGAVL
                     AAEFIKDKKGFFTMKDVLNL"
     gene            5923..7668
                     /locus_tag="PK977_10150"
     CDS             5923..7668
                     /locus_tag="PK977_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF18523.1"
                     /translation="MAQPDTDSLRRVMQSNRPYEQQINAAIQLVESYHLKNFDSTIIV
                     GDKALQMARKKADSSSVAKLKHQIGVASYFSGKYDVAAKNFIESISIFEKNDDKKKLA
                     PVYNDLAKLYRKTKDLDKALSNYNKADAIYRQLNDTAGIAMILNESGVVFEYRNDYKE
                     AINRYTSSMKLAEKAGDSLSVSYSLSNIAGVYVIEKKYDLAIENLLRALNIRQLLKDS
                     FAIALTYSDLGVAMNGKGDYSKAIEYLTLSNKMAEKLKYPELQSNNYNELSSVAQKQG
                     DFQKAFEYFNKRSALRDSLFTLEKTKEIQKLNSQYESARKEQQIQEQKNRLRMQNYLF
                     IGIAGLILLLALLAHSQYKRYKLKKESQLQAELMKQQEEAARAILEAEEKERQRIAKD
                     LHDGVGQMMSAAKMNLSAFESEMKYTNEEQKKALEKVIQLVDESCREVRTVSHIMMPN
                     ALLKNNLATAIQEFVNKLSSKTLQVDVYTEGLEERLDSNVETVLYRVVQECVHNALKH
                     AGATTLDISLIRDKDGISGTIEDNGKGFDPNDKENFEGIGLRNIITRIEYLKGTVDFD
                     SAPGRGTVIGIHVPL"
     gene            7837..8526
                     /locus_tag="PK977_10155"
     CDS             7837..8526
                     /locus_tag="PK977_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18524.1"
                     /translation="MAAKTKFIVFFKPEPLMKNGKITLALVDDHQIVIDGLTSLLKGH
                     DKFNFAFATTNSGEVIEKMADTPVDVLLTDVMMPKLPGNQLAKQVKEKYPKVKILALS
                     MSGQGDLVNEMINDADISGYVLKNIGKQELVTALEKIANGGIYFSEEVIDELQRASNR
                     RKQKEEAHLTGREVEIIQLIEKEYNNKQIAETLYISERTVETHRKNIFRKTNTNSVIG
                     LVKYAYEHKLI"
BASE COUNT         2716 a         1976 c         1774 g         2143 t
ORIGIN      
        1 ttcctccctg gaataaattc atcttctgcc cgtcatctac caaataaccc gtcgctgtca
       61 gtccaaaacc gcttttatga taatagccca acatcggtga gtaagtgagc tttttagtag
      121 tggtaattgt tgctgccgat tttgattcaa agttgtaata ccccttccct attgacaaac
      181 tgctcagaaa ataactgttg ggagaaagaa tagagtccat aaaggcatca aaatccctga
      241 aaagggcatc ataatcaatt gtcgtatcaa ttagtggaac ctctttgact ggcacaatta
      301 cggaatcgac cttattgtct tgtgcaaatg tatttacaca caacnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nactttatta gcatcaggca gttacgtcgg
      421 tcttccggat attggcggct tttcctaaaa tgatctggaa gcggctttta tgactaatta
      481 gttaaaaaat ggtttaatgc gttttctgat gaatttgaaa acgaaaaaaa cacccaaaat
      541 attgagagag aatcgataaa aaaacgatca ggacagctcc aaaactgtga tctcaggtaa
      601 aattcccacc cggccgggat agccaataaa accaaaacca cggttcacat agagcttttg
      661 agcctgagtc ggaccttcat ttgcctcttt ttcatacaaa cctgcccatt cgcggtaaac
      721 atattgtacc gggctccatt ttatgccggg aatctctacc ccaaactgca tgccgtgggt
      781 atggcctgaa agcacaaggt taacgtcagg gtaagaggga accacttctt ccttccaatg
      841 agaaggatca tggctcatta agattttaaa gggatagttt tcagttccgg catatgcttt
      901 ttccagatca ccatacttgg gaaacctcgc ttttgcactc cagttttcaa tacccaataa
      961 ggcaatcttg tccccatctt tctctaaaac aacatgctca ttcatcaaca acctccaccc
     1021 catggccgca tgtacttgtt tcagccgttc caggttggct tttttatctt cctcactgtc
     1081 ccaattcaca tattcaccgt aatcatggtt gccaagggta gaataaaccc caagcggagc
     1141 ctttagttta tcgaagacat taatgtaatc attcatttcc tcggccacat tatttaccag
     1201 gtcgccggtg aaaagaatca aatctggttt ctctttcagg attttttcca ccccacgcat
     1261 cacagcctgt ttatccgtaa aactgccgga atgaatatcg gagatatgaa caattcttaa
     1321 tcctttaaaa gacgaaggca ggttatcaaa tgacagcttc acttttttta cctggtaccg
     1381 gtatttgtta ccaaaaccat ataccaatgt gccaaataaa gtacctccca tcatcatacc
     1441 tgcccagcta agaaaagccg accgggtaat attctcgcca gactgcaaat ctgctccttc
     1501 cgttctttga aaaaaaagct tgccggctac ccattgcacc ccccttcgaa ggtcgtcgag
     1561 tacaaaaaag aaagcggcta ttaatttggc gaaaaacaaa ccggcaataa tggagaaaat
     1621 agtagtacgg gcaaaccggg cctgcttttc aaaatggata taaggcagaa tcatcagtac
     1681 aatgatggcc gcaactgata aaacccagta actagtataa agaatggttc gggtacgggg
     1741 agaggcagaa tgtgctacca ccttcagtgc ctggaaaaaa taaatatcca gcaaggccat
     1801 gaaaccgatc aaaatccacc aaaaaggagt attacgcata aggaataaac atcaaattta
     1861 agctggccta tgttatgggc attgtttggc ttaacactta tttttgccat ccttcaaatt
     1921 tcacagtatg aagtacacct attatggcca ttcctgcttc ctggtggaaa caaatggtaa
     1981 aaagcttttg ttcgacccat tcatcagtgg taatgaactg gcaaagaata tcaacattgc
     2041 cggtatagaa gctgattata tacttatatc acatggtcat gtcgatcata ttgcagactg
     2101 tgtagccatt gcacagtcta ccggcgccaa agttgtatgc agttgggaga tttacgaatg
     2161 gctgggcaaa aagggagtaa ctaaagctca cccgatgaat accggaggcc aatggcattt
     2221 tgagggtttc agcgttcgtt gcgtagtagc ccagcattcc agcgggttac ccgatggaag
     2281 ctacggtggt aaccccatgg gttttatcat ctcaaatgaa gaaggaagct tttattatag
     2341 cggagataca gccctgacac ttgatatgca actgatccca aggtgggcaa aactaaattt
     2401 tgcggtctta cccattggtg acaactttac catgggctat gaggatgcgg tggaagctgc
     2461 ccggatgatt ggctgcaata ccatcattgg tgtacattac gatacatttg gctttataaa
     2521 aattgaccat acaaaagcaa ccgttacgtt tgaaaaagct ggttgcaaac tgcatttagt
     2581 agaaatcgga aagagcatta cgttataaaa tacaggatta ctgaaaatgg gaagaatcat
     2641 tggcgttgca aatcagaaag gaggtgttgg caaaaccact accgctatta acctggcagc
     2701 aagttttgcc gtactggagt ataaaacatt attggtggat ggtgatccac aggccaacag
     2761 tacgacaggg gtcggctttg atctccataa tatcactcag agcctttatg actgtatggt
     2821 taaccacgca aaggcaaaag atgtgatcct gaaaacggaa atccctaacc tggaccttat
     2881 tccttcgcat atcgaccttg ttggtgcgga aattgaaatg atcaactacc ccaacaggga
     2941 aatggtgctc aagaatattc tggaagctat ccgcaatgaa tacgatttta tcatcattga
     3001 ctgctctcct tctcttggat tgataaccgt aaatgcactt actgctgctg actcagttgt
     3061 aattcctgtg caggcagaat tttttgcact ggaaggtttg gggaaattac tcaacaccat
     3121 caagatcgta caaagccgtt tgaatacatc gctggttatt gaaggcatat taatgacaat
     3181 gtacgatgga cgtctccgcc tttgtaacca ggtagtaagc gaggttcgcc gtcactttga
     3241 tgacatggta ttcagtaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnaagg gttcggttaa ctacctgaac cttgccaagg
     3361 agatcctgca aaaaaacgat atgaccaaaa ttaaaaacga ggaaaggatt ttagagctgt
     3421 aacaagttta aagtttaatg ttaattacct aatgcagaaa ctcaacctta aactctaaac
     3481 cctaaaccaa caacatgacc aaccccaaac aaaataaaga actacttggt aaaggcatcc
     3541 gttctttgct gcagagtatc gactcagacc tgaaaacaag cagcgggcac ctgaagtcaa
     3601 atgtggtgga aaatgtaacc gggattaatc gtatcccgat tgcagatatc gaagccaatc
     3661 ccaaacagcc ccgtcgtgat tttgatgaaa aggcattgca ggaaaccgtc tactattttg
     3721 cgagcaatat aggcaaaatg catgacatca ttcagccggt cactgtttca aaactgcctt
     3781 ccgggaaata tcggctgatc tccggcgaaa gaagatggag agcatccaaa ctggccggca
     3841 tccaggatat tccggcttat gttcggcaag ccaatgatgc acaattactg gaactggccc
     3901 tgctagaaaa cctgcaaagg gaagacctga atgctatgga aattgccctt agttacaaac
     3961 gaatgatgga ggaactggac ttcacacaag agcaagtagc agaaagaatg ggaaaagacc
     4021 gaagtacggt agccaacttc atccgcctgc tgaaactgcc acctgatatt caactggccg
     4081 tcagaaacgg agaattgagc atgggccatg ccagggcttt gataaatgtt gatacaattg
     4141 acaaacaact ttacattttc aaagaaataa aaaacaaagg gctttctgta cggcaaacag
     4201 aggccttagt aagaaacctt tataaagatg gcgctgttaa aaaaacttca aaaagccagt
     4261 tacatggccc ctttcagcga attgaagata aattagcatc gcatttcagt acccgggtaa
     4321 aactcaggca cagccggaac ggcagcggac agatcaccat tgattattat tcaacagaag
     4381 agttgaacaa gattctccgg caaatgaatg caccgcttga ttaatcatga gaatagcttc
     4441 agtaatcatt ttttcgttaa tcattctcac ttcttcttgt ctatatgcac aggaaaaagt
     4501 acctgttgct gtttctgcca aagactcaat tcctgtaact ggcaaaaaga aaaatatagg
     4561 cagtcgttac gattctttga gcaaggcaca tagtccccgc aaagcagcta ttcgttctgc
     4621 cttattgccg gggcttgggc agatctataa taagaaatac tggaaactgc caatcgttta
     4681 cggtgcattg ggcacctgcg ccggtatttt tatttacaat ctcggtaact ataaagacac
     4741 caagttcgct taccgtgtca aatacaatat gcgggttttc cgtaccgact ctgcttcttt
     4801 tgtcaatatc aaagacaacc tgaaaccgct cagtgaggaa tcactcaagt tttacagaaa
     4861 ccagtttcgt cgtgatattg attattctgc acttgttttt attttgttat ggggattgaa
     4921 tgtagtggat gccaccgtag acgctcatct taaaagtttt gatgttagcc ccgaccttag
     4981 tctccggata aagcccggct atagtgacct ggccaggaca aatggcttga gtttgatcct
     5041 gaagattggc aaataaaatc cgtactggca cgggtacctt cgccgttatt aataattaca
     5101 tttgtccaac gacattagct gttgtacatc aacattaaac ttgaaacatg aaatttgtat
     5161 taattggttt cggcaaaatg ggtaaggcca ttgaagaagt ggccctgcaa cgtggccacc
     5221 aggtaatatt gaaaatcgat cagcccaacc tgcatgaatt cactaaagaa aaaatagccc
     5281 ttgctgatgc agccattgaa ttcaccagtc cgcacagcgc ctatgagaat gtgaaaaagt
     5341 gtattgattt tgatataccg gttgtctgcg gctccaccgg ctggacggaa aaactgaacg
     5401 aaatgaaaaa acatgctaca gaaaaaaacg ttggctttgt ttactccagc aattacagtg
     5461 ttggtgtcaa catttttttt gaagtgaata aaaagctggc tgaattaatg gccccgcaca
     5521 aagaatacga agtgatactg gaagaaacgc atcacaccca aaaaaaagat gcgcccagtg
     5581 gcacagctat tacgctggct gagcagatcc tggaaaaagt gaaaaggaaa aagcaatggg
     5641 taaacgaact cagtgataat ccagaggacc tcgagatcat cagtcagcgg gtagaccctg
     5701 cccccggcac acatagcgta aaatactcct ctgttattga taatatcgag atcatacaca
     5761 ctgcccatag ccgcaccggc tttgccacag gtgccgtact ggctgcagaa ttcatcaaag
     5821 acaaaaaagg tttcttcacc atgaaagatg tattaaacct ctgatctttt catgcagcga
     5881 ttcagccttt tcatatcatt ccttatcctt tccttgtgca gcatggctca gcctgatacc
     5941 gacagcttgc gcagggtgat gcagtccaac aggccatatg aacagcagat caacgcagct
     6001 attcagctgg tagaaagtta ccatctgaaa aattttgact ctactattat tgtaggcgat
     6061 aaggcgctgc aaatggcccg caaaaaggct gattcatcca gtgtagccaa actcaaacac
     6121 cagatcgggg tagccagcta cttcagcggc aagtatgatg tagccgccaa aaatttcatc
     6181 gagtccattt ccatctttga aaaaaatgat gacaaaaaga aactggcccc tgtttacaac
     6241 gatctcgcca aattgtatcg caaaacaaaa gaccttgata aggcacttag caattacaat
     6301 aaagccgatg ccatctaccg gcaactgaac gacaccgccg gcatcgctat gatattaaat
     6361 gaatctggag tagtatttga ataccggaac gactataaag aagctatcaa ccgctatact
     6421 tcttccatga agctggcaga aaaagccggc gactctctca gcgtttctta ttcgctcagc
     6481 aatattgccg gtgtatatgt gatcgaaaaa aaatacgatc tcgccattga aaatctgctc
     6541 agggctctca acattcgcca attgctaaaa gatagttttg ccattgccct cacctactcc
     6601 gacctcggtg tggctatgaa tggcaaaggc gattatagca aagccattga atatttgacg
     6661 ctgagcaata agatggcaga aaaactgaag taccccgaac tgcagtccaa caattacaac
     6721 gaattatcct ccgtagcaca aaagcagggt gattttcaaa aagcctttga gtacttcaac
     6781 aaacgatctg ccctccgtga cagccttttt actcttgaaa aaacaaaaga gattcaaaag
     6841 ctgaattcgc aatatgagtc tgcccgtaaa gagcaacaga tacaggaaca aaagaaccgg
     6901 ctgcggatgc agaattattt gttcatagga attgcaggcc tgattctctt attggcttta
     6961 ctggcacatt cgcaatacaa acgctacaag ctgaagaaag aaagccagtt gcaggccgag
     7021 ttgatgaaac aacaggaaga agccgcccgg gccatcctgg aagcagaaga aaaggaaaga
     7081 cagcgcatcg ccaaagacct gcatgatggt gtgggccaaa tgatgagtgc cgccaaaatg
     7141 aatctgtcgg cctttgaatc cgaaatgaag tataccaatg aagagcaaaa aaaggccctg
     7201 gaaaaagtga tacagctggt tgacgaaagc tgccgcgaag tccgcactgt ttcccacatc
     7261 atgatgccta atgccctgct caaaaacaac ctcgccaccg ccatacagga atttgtgaac
     7321 aagctcagca gtaaaaccct gcaggtagat gtgtacaccg aagggctgga agaaaggctt
     7381 gattccaatg tggaaactgt cctttaccgg gtggtacagg aatgtgtgca taatgccctg
     7441 aaacatgccg gcgccaccac cctcgacatt tccctgatcc gcgacaaaga cggcatcagc
     7501 ggtactattg aggacaatgg taaaggcttc gatcccaacg acaaagagaa ctttgaaggc
     7561 atcggcctcc gcaatatcat tacccgtatt gaatacctca aaggcaccgt cgatttcgac
     7621 tccgcccccg gccgcggcac cgtcatcggc atccatgtcc ccctataacc cgaatcccgt
     7681 atcccgaaac ccgcatcccg catcccgtat cccgtatccc gttgtcaccc tgagcttgtc
     7741 gaagggccac tctccatttt cctgtccttt aacatttccc caactgcaat ctccccaacc
     7801 cctgcatcca tattggcgtc agtcagaaaa aacagaatgg ctgcaaaaac caaattcatc
     7861 gtatttttca aaccagaacc actcatgaaa aacggaaaga tcacactagc cctggtagat
     7921 gaccaccaga ttgttatcga cggactaacc tcgttactga aaggtcatga caaattcaat
     7981 tttgcctttg ccaccaccaa ctccggtgag gtaatagaaa aaatggccga tacaccggta
     8041 gatgtactgc tcaccgatgt aatgatgccc aaactgcccg gcaatcagct ggccaaacag
     8101 gtaaaagaaa aatatcccaa agtaaaaata ctggccctct ccatgagcgg acagggcgac
     8161 ctggtcaatg aaatgatcaa cgatgccgac atatccggtt atgttctcaa aaatatcggc
     8221 aagcaggaac tggtaaccgc actggaaaaa attgccaatg gcggtatcta cttcagcgaa
     8281 gaagtaatag atgaactgca gcgggccagc aaccgccgca agcaaaaaga agaagcccac
     8341 ctaaccggcc gtgaagtaga aatcatacaa ctgatagaaa aagaatacaa caataaacaa
     8401 atagccgaaa cactatacat ctccgaacgc accgtagaga cccaccgcaa aaacatcttc
     8461 cgcaaaacaa acaccaactc cgttatcggc ctggttaagt atgcttatga gcataagctg
     8521 atataaattt tctacgaaac ccttctgcaa tacaaattgc gaacggtagt tgtattgcca
     8581 gaattgcaat gaaaattgcg aattgcaata acttgatttt caagtgatgt gatttaaaat
     8641 aatttcggag agtgtcgtat atttatgtgt tgcctgctat attatgtaac ccctctactc
     8701 caacatcttt tcataatact ata
//
LOCUS       DAORLS010000120         8685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4396_length_8685_cov_2.256895, whole genome shotgun sequence.
ACCESSION   DAORLS010000120 DAORLS010000000
VERSION     DAORLS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4396_length_8685_cov_2.256895"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="PK977_10160"
     CDS             complement(<1..1819)
                     /locus_tag="PK977_10160"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18525.1"
                     /translation="MKILFSVLLLLFSVLAQAQILNRVKDRVTNKAKGEVNNAKNNAK
                     YKARESARNELNEVKAEFDSTDVDYAILLSDNSGLFGGKGKGEFGAKFLRLGGIANSL
                     YRDVDLDDDETATLNLQMGQSAYAMGKYVFAEKRFKAAQRYFENSYRTSELGYLKTIA
                     SQGLLYTTTGRFAQAENYTAEALKLRQEKLGKNNMAVAASLNNYAVLHYNIGLYNESE
                     REFASAIAVIKENKQDKAMAYAIVLNNKAMLFQSIGRYEEAVSLLKEALQLAGQLEVS
                     KARNHLKFFSNLALLYQQMGKYPEAEEIYRGLEKRLERGKPEFANLLNNVAILALLMK
                     KEDKVELMLKQSADIYKSTLGENSPAYAKVISDLGNFYRYKARYAEAEPLLEKALQIR
                     EQSLGDVHPLYVQSKEDMALLLWKKGEKEKAVPLYKEVMTKTIDFINRYFPPMSEAEK
                     TKYWDMLSPRFERFYNFVLDASASDKQLLTDMLEYRIATKGLLLSSSRKVSESILNSG
                     NEKLITDYNEWIDSKEQLTALYAYSKEELKEQAVNIDSLESAVNQMEKRLSQNSKDFA
                     DFFFTSKVKFSDIQKELKADEALVEIIRLRKYEQVLSTDS"
     gene            1912..2370
                     /locus_tag="PK977_10165"
     CDS             1912..2370
                     /locus_tag="PK977_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18526.1"
                     /translation="MKKYLLFIALIFVFQSSFSQTCEEREDKLLSAVGGFSAGLLYNT
                     YGLVGSVADAFGKDAYTAETVTDLMNAQKNMADNIIKMLDGLIKDNALKDENDKNYVI
                     ATKEIIAGLKKQAELLADYAKTNSSQQQRAYEQQRQKNWKDISKLMGIEE"
     gene            complement(2373..>3341)
                     /locus_tag="PK977_10170"
     CDS             complement(2373..>3341)
                     /locus_tag="PK977_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF18527.1"
                     /translation="RNNDNAELTSSKIQNTKVNLAGQVKEPVLCFLVIGNGNPVEVYV
                     EPGKISVKGKKENPSSYEISGSNSHKEFTGFTSSFVPKVQQLSATAAVINRTMLGKER
                     DSLMNIYTGLQGQLQDEIDRFVKQKPGSSVTPFILSATYGFNEDINRLESRYNQLTAD
                     AKNSEAGQQLAGFIAKEKVGAVGTEAMDFTQPDTLGNPVSLSSFRGKYVLIDFWASWC
                     GPCRQENPTVVENFNYFKDKNFTVLGVSLDRPGQKEKWMEAIHADNLTWTQVSDLLFW
                     NNAAAQLYNVKGIPQNFLIDPNGKIIAKNLRGPALREKLCEVLGCN"
     assembly_gap    3342..3379
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3550..>3773)
                     /locus_tag="PK977_10175"
     CDS             complement(3550..>3773)
                     /locus_tag="PK977_10175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRF18528.1"
                     /translation="ASGSIESYLERLRGLQLKNPVLVGFGIKDKQSFDAVCQLAEGGI
                     IGSAYIKALENSADVNASTKSFLSSILGR"
     assembly_gap    3774..3802
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3803..4321)
                     /gene="trpA"
                     /locus_tag="PK977_10180"
     CDS             complement(<3803..4321)
                     /gene="trpA"
                     /locus_tag="PK977_10180"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRF18529.1"
                     /translation="MKRIQELFRNKTSNVLNIYCTAGYPRLHSTLEVMRALQENGADI
                     IELGMPYSDPLADGPVIQHSSSVALANGMTIAILFEQLRDFRNSIHVPVVLMGYMNTV
                     LQYGFERFCADAAAAGVDGLILPDLPEYEFEIEYGAIIKKYGLDFIFLVTPETTDERV
                     KKLDSLSSGFLYA"
     gene            complement(4346..5545)
                     /gene="trpB"
                     /locus_tag="PK977_10185"
     CDS             complement(4346..5545)
                     /gene="trpB"
                     /locus_tag="PK977_10185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRF18530.1"
                     /translation="MTSQQTYRQPNEQGYYGQFGGAYIPEMLHRNVEELRTRYLEIME
                     EEGFQKEFQQLLRDYVGRPTPLYFAERLSNRYKANIYLKREDLCHTGAHKINNTIGQI
                     LLAQRLGKKRIIAETGAGQHGVATATVCALKGVECIVYMGEKDIERQAPNVARMKMLG
                     ATVIPATSGSKTLKDATNEAIRDWINNPNDTHYIIGSVVGPHPYPDMVARFQSVISEE
                     MRGQLLEKTGSELPKYVIACVGGGSNAAGAFYHFLDEPSVQLIAVEAAGEGINSGKSA
                     ATTALGKPGILHGSKSLLMQTHDGQVVEPHSISAGLDYPGIGPLHAYLFETGRATFMS
                     ATDKKALNAAFELAKYEGIIPALESAHAMHALKMINFNPKDVVVICLSGRGDKDMSAY
                     LSEINKL"
     gene            complement(5761..6174)
                     /locus_tag="PK977_10190"
     CDS             complement(5761..6174)
                     /locus_tag="PK977_10190"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HRF18531.1"
                     /translation="MKRRIILYNPKLKELARKLRQNMTFSEVKLWNELKNGQLLGYDF
                     DRQRPIGNYIVDFFCKDLQLAIEVDGITHLDEKVIEKDEIRQEELESKGICFLRFDAL
                     LVVDKVEVAVREIREWIEAYEKQYGASEFALKKRK"
     gene            complement(6296..>6632)
                     /locus_tag="PK977_10195"
     CDS             complement(6296..>6632)
                     /locus_tag="PK977_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRF18532.1"
                     /translation="EVIKAFRITGNEDIDKLVAPYDAVCDYYLFDTGGLKESFGGTGQ
                     QFDWNILSNAKIEKPFFLSGGIGVEDAAKVKAFKHPDLFGVDINSKFESAPGVKDMKL
                     ILQFKQAMK"
     assembly_gap    6633..6679
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6680..6925)
                     /locus_tag="PK977_10200"
     CDS             complement(<6680..6925)
                     /locus_tag="PK977_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRF18533.1"
                     /translation="MNIKICGITEMKQLQQLDGLDIDFAGLIFYPDSPRYVGDKLNKK
                     EIKKADFDLKKVGVFVNPEMIDVLDAIDDYGLEVVQLH"
     assembly_gap    7034..7082
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7083..7831)
                     /locus_tag="PK977_10205"
     CDS             complement(<7083..7831)
                     /locus_tag="PK977_10205"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRF18534.1"
                     /translation="MSTILETIVATKRKEIEPFKTRSPIERFEREGFFWEIRNRSQVQ
                     SLLLPGSTGIIAEFKRKSPSKGWFKTKELEVEPVVAAYNKSAAGISVLTDDEFFGGDL
                     DDLIQTKVVTDIPVLRKDFIIDKWQIAESKAFGADVILLIAACLSPAQVKEFAVYAAS
                     IGLESILEVHNEDELGHYCDEVSMIGVNNRNLNTFEVDIKTSLELIDKIPGGKPAIAE
                     SGISSVDTILTLRDAGFKGFLIGENFMKEEQP"
     gene            complement(7828..>8685)
                     /gene="trpD"
                     /locus_tag="PK977_10210"
     CDS             complement(7828..>8685)
                     /gene="trpD"
                     /locus_tag="PK977_10210"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRF18535.1"
                     /translation="RSITIEELQGFRDALLELCVKTELGEYETLDIVGTGGDGKNTFN
                     ISTLACFIVAGAGQKVAKHGNYGASSISGASNVMEQLGYQFSHDNGKLKRELNEANIC
                     FLHAPLFHPALKTVGPIRKNLAMRTFFNMLGPMANPANPAYQLVGVYNLEMARIYNYL
                     LQSGGKAFTIIHSLDGYDEISLTNDTKVITNNGEKIMTPEQLGKRMVSAADINGGTTV
                     EEAAKIFKTIMKGEGTWAQNAVVLANAAMALYCTGKYANYEDAYKSAVESLESGAANN
                     CLNKLISLQ"
BASE COUNT         2247 a         2101 c         1538 g         2636 t
ORIGIN      
        1 ggctgtcggt actcagcacc tgctcgtatt tgcgcagccg gattatttct accaatgctt
       61 cgtcggcttt cagttccttt tgtatatcac tgaactttac tttggaggta aaaaagaaat
      121 cagcgaagtc ctttgaattt tgcgacaatc ttttctccat ctggtttacg gctgattcga
      181 gtgagtcaat atttacagcc tgttctttca gttcttcttt tgaatatgca tacagggccg
      241 tcagttgttc cttgctgtcg atccattcat tataatcagt aattaatttc tcattcccgc
      301 tgttaagaat actttcactc actttccggc tggaacttaa caacaatcct ttggtggcga
      361 tccggtattc cagcatatca gtcagcagtt gtttgtcgct ggcagaagca tccaatacaa
      421 aattgtaaaa ccgttcaaag cggggcgaca acatatccca atatttcgtt ttctccgctt
      481 cgctcattgg cggaaaatac cggttgatga aatcgattgt tttagtcatc acttctttgt
      541 aaagtggcac ggccttttct ttttctccct ttttccagag aagcagcgcc atatcttctt
      601 ttgattgtac atataacgga tgcacatcac ccaatgactg ttctcttatt tgcagggctt
      661 tctcaagcag tggttctgct tcagcatatc ttgccttata gcggtaaaaa tttcccaggt
      721 cgctgatcac tttggcataa gccggtgagt tttctcccaa agttgattta taaatatctg
      781 ctgattgttt cagcatcagt tctactttat cttccttctt catcagcaaa gccagtatcg
      841 ccacattgtt caacaggttg gcaaactcag gtttgcctct ttctaatctt ttctctaatc
      901 cacgataaat ttcttccgct tccgggtact ttcccatttg ctggtacagc aatgccaggt
      961 tggagaaaaa cttcaggtga tttcttgcct tgcttacttc taattgaccg gccagttgca
     1021 gggcttcttt cagcaaactc actgcctctt catagcggcc gatcgactga aacaacatgg
     1081 ccttattgtt cagcacaata gcgtaggcca tggccttatc ctgcttattt tctttgatca
     1141 cagcaatggc agatgcgaat tccctctctg attcattgta caggccaata ttataatgca
     1201 gcaccgcata gttatttagt gaagcagcca cagccatatt attctttccc aatttctctt
     1261 gccggagttt caatgcttct gctgtgtaat tctcagcctg tgcaaatctt ccagtggtgg
     1321 tgtagagcaa tccctggctg gcgatcgttt ttaaataacc cagttcactg gtacggtacg
     1381 agttttcaaa ataccgctgt gctgctttaa atcttttttc agcaaacaca tacttaccca
     1441 tcgcataggc tgattgcccc atttgaagat tcagtgtggc agtttcatca tcatcgaggt
     1501 ccacatcccg gtataatgaa ttggctatac cacccaatcg cagaaatttg gcaccaaact
     1561 cacctttgcc ttttccgcca aacaaaccgg aattatcact cagtaagatg gcataatcca
     1621 catcagtaga atcaaactcc gcttttactt cattcagttc attccgggcc gattcccttg
     1681 ctttatactt agcattattc ttggcgttat ttacttcacc cttggctttg ttggttaccc
     1741 ggtcttttac ccggttgagt atctgggcct gtgccagcac tgagaataaa aggagtaaaa
     1801 cagaaaagag aattttcata cacgaatttt aggtttcagg gtcaaaaact tccctaaatt
     1861 acattttatt ccatgtactt attatataac aatcttgtta aaaccggaat tatgaaaaaa
     1921 tacctgttat ttattgcgct gatatttgtt tttcagagca gtttttctca aacctgtgag
     1981 gaaagagaag acaaactgct ttcagcagtg ggaggattct ctgccggctt gttatacaat
     2041 acgtatggcc tggtaggaag tgttgccgat gctttcggca aagacgcata cactgcggaa
     2101 accgttactg acctgatgaa tgcgcaaaag aatatggccg acaatatcat taaaatgctc
     2161 gacggactga tcaaagacaa tgccctgaaa gatgaaaatg ataagaatta tgttatcgcc
     2221 acaaaagaaa ttattgccgg gttaaaaaaa caagccgaat tactggcaga ttatgctaaa
     2281 acgaacagca gccagcaaca gagggcttac gaacaacaac gccagaaaaa ctggaaagac
     2341 attagcaaac tgatgggaat tgaagagtaa gatcagttac aacccagtac ctcacaaagc
     2401 ttttccctta atgcaggccc ccgcaggttc ttagcaatga tttttccgtt gggatctatc
     2461 aaaaaattct gcggtatccc ttttacatta tacaactgtg ctgcggcatt attccagaat
     2521 aacaagtcgc ttacctgtgt ccatgtaaga ttatcggcat ggatagcttc catccatttc
     2581 tctttctggc cgggacgatc cagcgatacg ccgagcactg taaaattctt atccttgaaa
     2641 taattgaaat tctctaccac tgtcgggttt tcctggcggc aggggccgca ccagcttgcc
     2701 cagaaatcta tcaatacata tttaccgcga aaagaagaaa gcgaaacagg gtttcccaac
     2761 gtatccggct gggtgaaatc cattgcctcg gtacctacag cgcctacttt ttctttggca
     2821 ataaatcctg ccagttgctg accagcttct gaatttttgg catcagcagt cagctggtta
     2881 tacctgctct ccagcctgtt gatatcttca ttgaacccat aagtggcgct gaggataaaa
     2941 ggggtaacag aagagccggg cttttgctta acgaaccggt ctatttcatc ctgcagttgg
     3001 ccctgcaatc ctgtataaat attcatcagg ctgtctcttt ctttacccaa catggtcctg
     3061 ttgatcacgg cagctgtagc agatagttgc tgcactttcg gaacgaaaga agaagtgaac
     3121 cctgtaaact ctttgtgtga attggaaccg gatatctcgt aggaagaagg attttctttt
     3181 ttccctttta cggatatttt accgggttct acatatacct ccaccgggtt gccgttgcca
     3241 atcaccagga aacacagtac cggctctttc acttgccctg ccaggttaac ttttgtgttc
     3301 tgaatttttg aagaagttag ttccgcatta tcattattac gnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnna gccgtcaaac tttccttcaa tgacaaagga tttgttctgc
     3421 gcaaaggcag cgatcatgaa atttgataag agtactgcta aaaataattt cttcatacag
     3481 gcgcctgttt tcctttttgt aatattggac aagttgcaaa tttaaccaaa gccgttgaat
     3541 gaaaataccc taacggccga gtattgacga aaggaaggat tttgtggatg cattaacatc
     3601 tgcgctgttt tccaatgctt taatataggc gctgccgata ataccaccct cggccagttg
     3661 acaaacggca tcaaacgatt gcttgtcctt aataccaaaa ccaaccagca ccgggttttt
     3721 aagttgcaac cccctcagcc gttccaggta agattcaatg ctgccggatg cttnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnagcataga ggaaaccgga actgaggctg tccagttttt
     3841 ttacccgttc gtccgttgtt tcaggagtga cgaggaaaat aaaatcaagg ccgtattttt
     3901 ttatgatagc cccatattca atttcaaatt catactccgg taaatctggc aatatcagcc
     3961 cgtcaacacc tgctgcagcc gcatcggcgc aaaaccgttc gaagccatat tgtaaaacgg
     4021 tgttcatata acccatcagc accaccggca catgaatgct atttctaaaa tctcttagct
     4081 gttcaaaaag gatggctatt gtcatgccat tggccagtgc aacactgctg ctgtgctgaa
     4141 ttaccgggcc atctgccaat gggtcgctgt acggcattcc cagttcgatg atatcagcac
     4201 cgttttcctg caaagctctc attacttcaa gtgtggagtg caaacgggga taacctgcag
     4261 tgcagtaaat attcaaaaca ttggatgttt tattcctgaa taattcttgt atccttttca
     4321 ttcagcattc atcatttaac attaatcaca atttgttaat ttcggacaag taagctgaca
     4381 tatctttatc tccccttccg cttaaacaaa tcactaccac atcttttgga ttaaaattga
     4441 tcattttcag ggcatgcatt gcatgggctg attcgagtgc aggtataatg ccttcatatt
     4501 ttgccagttc aaaagcagca ttcaaggctt tcttatcggt ggcactcata aaagtagccc
     4561 tgccggtttc aaataaatat gcatgtaagg gaccgatgcc gggataatcc aaccctgctg
     4621 aaatactatg aggctccacc acctggccat cgtgagtttg catcagcaaa cttttgcttc
     4681 catgtaaaat acccggctta cccaatgcag ttgtcgcagc gcttttacca ctgttgatcc
     4741 cttctccggc tgcttccaca gcaatcaatt gtacagaagg ctcatctaaa aaatgataaa
     4801 aggccccggc agcattgctg ccaccgccta cacaggctat aacatatttc ggtaattcac
     4861 tgcccgtttt ttccagtaac tgtccccgca tttcttcact gatcacactc tgaaacctgg
     4921 ccaccatatc aggataagga tgcggaccta ccaccgaacc aataatataa tgtgtatcat
     4981 tcggattatt gatccagtct cttatcgctt cattcgttgc atctttcaac gttttgctgc
     5041 cgctggtagc aggaataacg gtggctccca gcattttcat cctggctaca ttcggagcct
     5101 gccgttcaat atccttttca cccatataca caatgcactc aacccctttc aatgcgcaaa
     5161 cagtagcagt ggccactcca tgctggccgg caccagtttc agcaatgatc ctttttttac
     5221 ctaaccgctg tgccagcaaa atctgtccta tagtattatt gatcttatgg gcgccggtat
     5281 gacaaaggtc ttcccgcttc aggtagatat ttgctttata tcggttactt aatctttcag
     5341 caaagtataa aggtgtcggc ctccccacat aatctctcag caattgctga aactcttttt
     5401 gaaaaccttc ctcctccatg atctccaggt acctggttcg cagttcctcc acatttcggt
     5461 gcagcatctc gggaatgtag gcgccgccaa actggccgta gtatccctgt tcattgggct
     5521 gccggtatgt ttgttgacta gtcattttac ttatagtttt tcctgattca cttttttatg
     5581 agattaacac acccaaattt ctcaaagcta aaaactcatt tagcttatct ctcctgcaaa
     5641 gggatgtttg ctaaaacaca gctttttcag tacttcggat agcagcggcc ttcatcccct
     5701 ccccggaggg gccgcagtct ttactcataa agaaatcttc aaatggggtg ggtcaaccaa
     5761 tcacttcctt ttcttcaatg caaactcact tgctccatac tgcttttcat acgcttcgat
     5821 ccattctctt atctctctca ccgctacctc cactttatcc acaaccaata aggcatcgaa
     5881 tcgtaaaaaa caaataccct ttgattctaa ttcctcttgc ctaatttcat ctttctcaat
     5941 aaccttttca tctaagtgtg tgatgccatc tacttctata gccagttgca aatctttaca
     6001 aaagaagtca acaatataat ttccaatcgg cctttgcctg tcaaaatcgt accccaataa
     6061 ttgtccgttc tttaattcat tccatagttt aacttctgaa aatgtcatat tctgccttag
     6121 ctttctcgca agctctttta actttgggtt gtatagaata attcgccttt tcattttgac
     6181 ccaccccggc taattaaagc agaaagttta ccaacttcac ccctccgggg aggggatgtg
     6241 aactaaagca caaagatcac tgccaattaa ctttcttcat ttttgaactc gacccctatt
     6301 tcattgcctg tttaaactgt aatatcaatt tcatatcctt cacccccggt gctgattcaa
     6361 acttactgtt gatgtcgacc ccgaaaagat cgggatgttt aaaagccttc acttttgctg
     6421 catcttctac accaataccc ccactcagga aaaaaggttt ttcaattttt gcattgctta
     6481 aaatattcca gtcgaactgc tgaccagtgc cgccgaaaga ttctttcaaa ccaccggtat
     6541 caaacaggta ataatcacaa acagcatcat aaggtgcaac cagtttatcg atatcctcat
     6601 tacctgttat gcgaaacgct tttatcactt ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnng tgcaactgta ccacttccag tccgtaatca tctattgcat
     6721 ccagcacatc gatcatctcc gggttgacaa acacacccac cttctttaaa tcaaaatctg
     6781 cttttttgat ttctttttta ttcaatttat cacccacata gcggggagag tcaggataga
     6841 agatcaatcc tgcaaagtca atatccagcc cgtcgagctg ctgtagttgt ttcatttcgg
     6901 tgataccgca aattttaata ttcatagttt ttcttttgta cacagataaa cacaaattga
     6961 ctcatataaa tatcaactta agttacagtg catgttattt ctaaattatt attcagtaat
     7021 tgtgttcatt tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnggttgctc ttctttcata aaattctcac cgatcaagaa tcctttgaaa cccgcatccc
     7141 gcaaagttag tattgtatcc acgctgctga taccgctttc ggcaatagca ggtttaccgc
     7201 cgggtatttt atcaataagt tctaatgacg ttttgatatc tacttcaaac gtattaagat
     7261 tccggttgtt caccccaatc atactcactt catcgcagta atgtcccagt tcgtcttcat
     7321 tatgcacttc aagtatcgac tcaaggccta tacttgccgc atatacagca aactctttta
     7381 cctgtgccgg tgacaggcag gctgcaatta acagaattac atccgcacca aaagcttttg
     7441 attcagcaat ctgccattta tcaatgataa aatctttacg cagcaccggt atatcggtca
     7501 ccactttcgt ttgtatcaaa tcatccaggt caccgccaaa gaattcatca tcggtcagta
     7561 cggaaatacc tgccgcactt ttattatacg cagcaacaac cggctccact tccagttctt
     7621 tggttttaaa ccagcccttg ctcggacttt ttcttttgaa ctcggcaatg atacccgtac
     7681 tgccgggtaa caaaaggctt tgcacctgtg accggtttct tatttcccag aaaaaccctt
     7741 cccgttcaaa ccgttcaata ggacttctgg tcttgaatgg ttcaatctct tttctcttgg
     7801 ttgctactat tgtttctaaa atagtactca ttgaaggcta atcaatttat tcaggcaatt
     7861 attggcagca ccactttcca ggctttctac tgcagacttg taagcatcct cataattagc
     7921 atactttcct gtgcaataca atgccattgc ggcattcgcc agcaccactg cattttgcgc
     7981 ccatgttcct tctcctttca ttatagtttt gaaaatcttt gcagcttctt ccacagtagt
     8041 ccccccattg atatccgctg ctgataccat ccgcttacca agttgctccg gggtcattat
     8101 cttttctcca ttgttcgtta tcaccttggt atcattggtc agcgatattt catcatatcc
     8161 gtctaagctg tgaataatag taaatgcttt accgcctgat tgtaaaagat aattgtagat
     8221 tctcgccatt tccaggttat atactccaac taactggtac gccgggtttg ccggattggc
     8281 cattggcccc agcatattga aaaaagtacg catggccagg tttttccgta tcgggcccac
     8341 tgtctttaat gcagggtgaa ataaaggtgc atgcaaaaaa caaatattcg cctcgttcag
     8401 ttctcttttc agtttgccgt tatcatggct gaactgatat cccaactgct ccatcacatt
     8461 actggcgccg ctaatggaag acgccccgta attaccatgc ttggccactt tttgtcccgc
     8521 acccgccact ataaaacagg ccagcgtaga aatattgaaa gtattttttc cgtcaccgcc
     8581 ggtacctaca atatccagtg tttcatattc acccagctct gtcttcacac ataattcaag
     8641 caatgcatcc cggaagccct gcaactcttc aatggtaata ctccg
//
LOCUS       DAORLS010000121         8611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4445_length_8611_cov_2.339060, whole genome shotgun sequence.
ACCESSION   DAORLS010000121 DAORLS010000000
VERSION     DAORLS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4445_length_8611_cov_2.339060"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK977_10215"
     CDS             <1..546
                     /locus_tag="PK977_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRF18536.1"
                     /translation="QPWPWYVAGPLIGLMVPALLIIGNKSFGISSSLRHVCAACVPAK
                     IPFFQYDWKKEIWNLFFVAGIVGGGFLAVSLLGNPDPIQVNPKLQAELATYGVTNYDS
                     FVPADLFNWQALGSIKGIIMMIVGGFLVGFGTRYAGGCTSGHAIMGLSNLQWPSLVAT
                     ISFMAGGFIVANLILPLILKL"
     gene            553..1086
                     /locus_tag="PK977_10220"
     CDS             553..1086
                     /locus_tag="PK977_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRF18537.1"
                     /translation="MSVNETLQEKKHHEFLTQNTDFEVRSLDAVCFNESELKHPWWYN
                     LKYAVAGILFGIILIKAEVISWFRIQEMFRLQSFHMFGVIGSAVAVGIISVWLIKKFK
                     VKTIYGEPIEFVDKKFNKGNIYGALIFGFGWAITGACPGPLFAQIGTGALVIIVTLFS
                     AIVGTWVYGKFRGKLPH"
     gene            1220..1693
                     /locus_tag="PK977_10225"
     CDS             1220..1693
                     /locus_tag="PK977_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HRF18538.1"
                     /translation="MKSNRIGLDAAKARKLAEKLNSLLANFQLFYINSRGFHWNIKGE
                     KFFELHAKFEELYTDTQLKIDEVAERILTLGHIPGHSYSDYLKLATIKERRNVHDGKE
                     AIKSVIDSFSILLEIERGLLELSADANDEGTNALMSDYIRQQEKLVWMYSAYLDK"
     gene            1706..3019
                     /locus_tag="PK977_10230"
     CDS             1706..3019
                     /locus_tag="PK977_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18539.1"
                     /translation="MKNFIIIITLLLSLNVSSQTNFPYKISLEPIIPEGFSGLQSYAW
                     AKSGNKILLVGGRTDGLHRRQPFASFNKKYNNTDLIVLDVKTEKVWKKSVTGLPSLLA
                     EQLQSTNMEFYQQQNDLIVVGGYGYSETKREHITHPAVLKIKVKEIIDGIINNKDITS
                     FIAQLTDERMAVTGGHLNKLDNLFYLVGGQRFDGRYNPHGPDHGPGFSQQYTNQIRKF
                     SITEKDRRLSIENYTAVTDSALLHRRDYNLLNQYDENGREMLTIYSGVFQYEKDLPYT
                     TLIDITANNHSEVENFNQQFSHYHSATLPVYDKNKKTLYTIFFGGIAQKYIDQSGVVK
                     TDNDVPFVKHISVVERNTNGIKEYLLPEQLSGYFGAAAEFIPADETLFSNNGIFQLEK
                     TGTKPLLAGYIIGGIDSRAPNIFWSNEAEPSRASPVIWKVYLSKR"
     gene            3016..4275
                     /locus_tag="PK977_10235"
     CDS             3016..4275
                     /locus_tag="PK977_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF18540.1"
                     /translation="MKSSDNIQTGLKENWQQFSLLVLVNAFVGAMIGLERAVLPGLAK
                     NIFGLDANTAILSFIMAFGITKAISNYSVAKLSKRFNRKNILITGWLFALPVPVLLMY
                     AETWTWVIIANILLGINQGLAWSTTVIMKVDIVGQKNRGLAMGINEFAGYLSVGLASY
                     LATSIATSNGYAYYPFVPGLFFAVSGLLISFFLVKDTTHFVHSESTVSKLALMKNVWK
                     ETTWKHKNIGTVTLNGLVNNMNDAVVWGLLPVLLLEKSFTIKEIGIIAGIYPVVWGVM
                     QLFTGKMGDVYCKKQIITAGMLLQATAIIILAAASVLWLLIIASILLGLGTALVYPNF
                     LTVVAESSHPSQRAETLSIFRFWRDSGYVIGALIAGVLADFIGLSAAIIVFAFITGIA
                     GLVAHFRMCCTLKKIYPSEDCFHPAVY"
     gene            4292..4864
                     /locus_tag="PK977_10240"
     CDS             4292..4864
                     /locus_tag="PK977_10240"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRF18541.1"
                     /translation="MLKSLFIITGLMTFLFSCKQSKNTEEFTDWKSKGDSLTAQTFDT
                     LRKTLQKAISERGLPGAVEFCNTAALDLTKIYSGKEIQIKRTSDKIRNPVNTPDEMEK
                     DVIMTYQEQKEENKQLTTIVKQDSKGNQHYFKPILIQAMCLNCHGAKETQIKPDTWDM
                     IQKKYPKDAAFNYKEGDLRGVWHIVFTKSK"
     gene            complement(4883..5476)
                     /locus_tag="PK977_10245"
     CDS             complement(4883..5476)
                     /locus_tag="PK977_10245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF18542.1"
                     /translation="MQQFWDQRYAENETVYGYEPNKFFKLFIDLHKPGSILLPAEGEG
                     RNAVYAASKGWQVDAFDFSEVAREKALDFARGENVLVNYDLKNITAFKAEKLYDAVGL
                     IYVHLPEEQRKKFHQEIYNSIKPGGFMVLEAFAKEQAQFESGGPRDTALLYDAPSLCN
                     DFPFLHLMSCEQKEINLNEGDYHKGKAAVLRIIGQRL"
     assembly_gap    5691..5798
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5799..>6064
                     /locus_tag="PK977_10250"
     CDS             <5799..>6064
                     /locus_tag="PK977_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF18543.1"
                     /translation="SQFKGKKVFVNLWATWCPPCRAEIPSIESLASKTDRSKVEFVML
                     SLDDDFVKAKIYAANNKMKLPVYYPTGNLPQLFNVEGIPTTFIF"
     assembly_gap    6065..6166
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6228..>6448)
                     /locus_tag="PK977_10255"
     CDS             complement(6228..>6448)
                     /locus_tag="PK977_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRF18544.1"
                     /translation="ELSKEYEGRLVIYKVDTDVEQELSAVFGIQSIPTLLFIDSEGEP
                     MMQPGAFPKHIFKKIIEEKLLAPVKTEE"
     assembly_gap    6449..6509
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6854..6867
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6868..8137)
                     /locus_tag="PK977_10260"
     CDS             complement(<6868..8137)
                     /locus_tag="PK977_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF18545.1"
                     /translation="MKVEQIYTGCLAEAAYYIESNGEAAIIDPLRETKPYIDKAERNG
                     AKIKYILETHFHADFVSGHIDLAKKTGATIVFGPTAAPAYDVHVAREGEVLQLGDVKI
                     KVLHTPGHTMESTTYLLIDEKGKDHAIFTGDTLFIGDVGRPDLVQKVKAEITPQILAG
                     YLYDSLRNQIMPLADEVIVYPGHGAGSACGKNMSKETTDTLGHQKKVNYALRSDMSKE
                     EFIKEVTEGLVEPPQYFPYNVLMNLKGGIESIDDIIARGKRPLNVKEFQTVWESMEAL
                     VLDTRAKEAFVEEHIPGSIFIGIDDNFAPWSGTLIMDLKQPILFLADEGREEEVVVRL
                     SRVGYDNAVGFLQGGIKAWKEAGLETDSIKEISANDFASLYQMDTEHINLLDARRKSE
                     FDSEHLMGAENFPLDFINQNMSQLSRDKKYF"
     gene            8457..>8611
                     /locus_tag="PK977_10265"
     CDS             8457..>8611
                     /locus_tag="PK977_10265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRF18546.1"
                     /translation="MQTKHQILIVGGGNAGISTASQLLRKNKNLDIAIIEPSDKHYYQ
                     PAWTLVGA"
BASE COUNT         2497 a         1679 c         1643 g         2507 t
ORIGIN      
        1 caaccctggc cctggtatgt agccggtccg cttataggac taatggtgcc cgcactgctg
       61 attataggta acaaatcctt tggtatttcg tcctcattga ggcatgtatg cgccgcttgt
      121 gtacctgcaa agattccctt ctttcaatac gactggaaaa aggaaatatg gaacctattt
      181 tttgttgcag gaatcgttgg tggcggattt cttgcggtta gtttattagg aaaccccgat
      241 cctatacaag ttaatccaaa attacaggca gaactggcaa cctatggtgt tacaaattat
      301 gactcctttg taccagcaga cctttttaac tggcaggcac tcggcagcat caaaggcatt
      361 atcatgatga ttgtgggcgg attccttgta ggttttggaa cccggtatgc tggtggatgt
      421 accagcgggc atgcaattat ggggttgtca aacctgcaat ggccttcact ggttgccacc
      481 atcagtttta tggctggtgg cttcatagta gcaaacctta ttcttccttt aattttaaaa
      541 ttataatata gcatgagtgt aaatgaaaca ttgcaggaaa agaaacatca tgaatttctt
      601 acacagaata cagattttga agttcgttcg ttagatgctg tttgttttaa cgagtcagaa
      661 ttaaaacatc cctggtggta taatcttaaa tatgccgtag cgggaatatt gtttggaata
      721 attcttatta aagcagaagt tatctcctgg tttaggattc aggaaatgtt caggttacag
      781 agtttccaca tgtttggagt aatcggttct gctgtagcag tgggaattat ctctgtgtgg
      841 ctgataaaaa aattcaaagt caaaaccatt tatggcgaac ccattgagtt tgtagataag
      901 aagtttaata agggtaatat ttatggtgca ttgatcttcg gatttggctg ggcaattacc
      961 ggcgcctgcc ctggcccgct gtttgcacaa atcggaacag gtgcattggt tattattgtc
     1021 acacttttta gtgccattgt ggggacatgg gtatatggaa aattcagggg taaattacct
     1081 cattaaaaat tagtttttat taatatttag cagtaagccg ggggattttt cccctggctt
     1141 tttcattcat cttaccgtga tgaaaatcac atctcaacca ggtgcaatag tttaatttgc
     1201 atgactaaaa cgtaaggcta tgaaatcaaa caggataggg ctggatgcag caaaagcaag
     1261 gaaacttgca gaaaaattga actctttatt ggccaacttc cagcttttct acattaattc
     1321 aagaggattc cattggaata ttaagggtga aaaatttttt gagctacatg ccaaatttga
     1381 agaactatac acagacaccc agttgaaaat tgatgaagta gctgaacgaa ttttgactct
     1441 cggccatata cctggtcact cctattctga ttatctaaag ctggcaacaa ttaaagagag
     1501 aaggaatgtg catgacggca aagaagcgat aaaatcagta attgactctt ttagcatact
     1561 gctggaaata gaaagaggat tacttgaact atccgccgat gcaaatgatg agggaactaa
     1621 tgccctgatg agtgactata tccgtcaaca ggaaaaatta gtctggatgt attcggctta
     1681 cctggacaaa taaataacca ttagaatgaa aaactttatt attataatta cactactact
     1741 aagtttgaac gtaagttcac agactaattt tccttataag atttctctcg aacccataat
     1801 ccccgaaggg ttctccggcc tccagtccta tgcctgggct aaatcaggga ataaaatact
     1861 gctggtcggt ggaagaacgg atggcctgca tcgaagacag ccttttgctt cctttaataa
     1921 aaagtataat aatactgatc ttattgttct ggatgtaaaa acagaaaaag tctggaaaaa
     1981 atctgtaacc gggcttcctt ccctccttgc tgaacagtta caaagtacca atatggagtt
     2041 ttaccagcag caaaatgatc tgatcgttgt tggcggctac ggttatagtg aaacaaaacg
     2101 ggagcatatc acacatcctg ctgttttaaa gattaaagtg aaagagatta tagacggcat
     2161 cattaataac aaggatataa cctcatttat cgcacaactg acagatgaac gtatggccgt
     2221 aacaggcggc catttgaata agttggacaa tttgttttac ctggtaggag gacaaagatt
     2281 cgatggccgc tataatccgc atggccccga tcacggaccc ggtttctcac agcaatacac
     2341 taaccagatc agaaaatttt caattacgga aaaagacaga aggctatcga ttgaaaatta
     2401 cacagcagtt actgattctg cattgttgca ccgccgagat tataacctgc tgaatcagta
     2461 tgatgaaaat ggcagggaaa tgctgaccat ttactccgga gtatttcaat atgaaaaaga
     2521 ccttccctat actacgctga ttgatatcac agccaacaat catagtgagg ttgaaaattt
     2581 caatcaacaa ttcagtcatt accattcggc tacactgccc gtatacgata aaaacaagaa
     2641 aacgctgtat actattttct ttggtggtat tgcacagaaa tatattgatc aatccggggt
     2701 agtaaaaaca gataacgatg tgccttttgt aaagcatatc agtgtagttg aaagaaatac
     2761 aaatggcata aaggaatacc tgttgccgga gcaattatcc ggttactttg gggctgctgc
     2821 ggaatttatc cctgcggacg agactttatt tagcaacaat ggcatttttc agttagaaaa
     2881 aacagggact aagccattac tggccggtta tattattggt ggaatagaca gcagggctcc
     2941 taatatcttc tggagtaatg aggctgagcc aagccgggct tcaccagtga tatggaaagt
     3001 ttatcttagt aaaaggtgaa aagcagtgac aatatacaga ccgggttaaa agaaaactgg
     3061 cagcagtttt ctctattagt gctggttaat gcatttgttg gtgcgatgat aggcctggaa
     3121 agagccgtac tacccggact tgctaaaaat atttttggac tagatgccaa tactgcgatt
     3181 ctaagtttta tcatggcgtt tggcattacc aaagccatca gtaactatag cgttgcaaaa
     3241 ctgagcaaac gattcaatcg aaaaaacata ctgattacag gctggttgtt tgccttacct
     3301 gtgccagtac ttcttatgta tgccgaaacc tggacatggg ttatcattgc caatatatta
     3361 cttggcatta accagggatt ggcctggtca actaccgtta tcatgaaagt ggatatcgtg
     3421 ggtcaaaaga accgcggact tgccatgggc atcaatgaat ttgccggtta cctttctgta
     3481 ggcctagcat cttacttagc cacttctatc gcaacaagca atggctatgc ctattatcct
     3541 tttgtaccag gtttattttt tgctgtttcg gggttgctta tttccttttt tcttgtaaag
     3601 gataccactc attttgtaca ttctgaaagc actgtcagta aactggcgct gatgaaaaat
     3661 gtatggaaag aaacaacgtg gaaacataaa aatattggta cagttacttt gaatggttta
     3721 gtaaataata tgaatgatgc agttgtttgg ggactgttgc ctgttctttt gctggaaaaa
     3781 tcgtttacca taaaggaaat tgggatcatt gcaggtatct accctgttgt atggggagta
     3841 atgcaactct tcaccggtaa gatgggtgat gtgtattgca aaaaacagat catcacagcg
     3901 ggtatgttgt tgcaggctac agcaattatt attcttgctg cagcatctgt tctatggctg
     3961 ctcattattg cttctatact tctagggctg ggaacagcat tggtttatcc taactttcta
     4021 acagtggtag cggaaagtag ccacccctcc caacgggcag aaacactgag cattttccgc
     4081 ttttggagag acagcggata tgtaatcggg gcattaatcg ctggtgttct ggcagatttt
     4141 ataggactaa gcgcagcaat aattgttttt gcatttatta ccgggattgc tggtcttgta
     4201 gcacacttca gaatgtgctg tacgttgaag aaaatctatc cctctgaaga ttgtttccat
     4261 cctgccgtat actaacttca aaaactggtc tatgctcaaa tctttattta tcattaccgg
     4321 cttaatgact ttcctttttt cttgcaaaca atcgaaaaac acggaagaat ttacagattg
     4381 gaaaagcaaa ggagattcat tgacggcgca aacatttgac actctcagaa aaacattgca
     4441 aaaagccatc agcgaaagag gcttacccgg tgcagtagaa ttttgtaata ctgcagcatt
     4501 agatctcaca aaaatttatt caggtaaaga aattcaaata aaacgaacct ctgataaaat
     4561 ccgtaatcca gtcaataccc cggatgagat ggaaaaggat gtgattatga cctaccagga
     4621 gcaaaaagaa gaaaacaaac aactaacaac aattgtaaag caagactcaa agggaaacca
     4681 acactacttc aaacccatac ttattcaggc aatgtgcctg aactgtcatg gagcaaaaga
     4741 aacgcaaata aaaccggata catgggatat gatccaaaaa aaatatccaa aagatgctgc
     4801 ctttaattac aaagaagggg acttaagagg cgtatggcat attgttttca ccaaatcgaa
     4861 atagtatacg ataattacaa tttcataacc gctggccaat tattcgaagt acggctgctt
     4921 ttcctttatg gtaatctcct tcattgagat taatttcctt ttgctcacaa ctcatcaggt
     4981 gcagaaaagg aaagtcgttg cacaatgatg gcgcatcgta caaaagtgcc gtatctctcg
     5041 gtccgccgct ctcaaactgt gcttgttctt tggcaaatgc ttccaatacc ataaagccgc
     5101 ctggtttgat tgaattatat atttcctggt gaaacttttt tctctgctct tccggcagat
     5161 gtacataaat caggccgaca gcatcgtaca gcttttccgc tttaaaggct gtaatatttt
     5221 ttaaatcata gtttaccaga acattttctc cccttgcaaa atcaagtgcc ttttcacggg
     5281 ccacctcact gaagtcaaaa gcatctacct gccagccctt tgatgcagca tacacagcat
     5341 tgcgcccttc accctctgct ggcaacagta tggagcccgg cttatgcagg tcaataaaca
     5401 gcttaaaaaa cttattaggc tcgtaaccat acactgtttc gttctctgcg tatcgttgat
     5461 cccagaattg ctgcatttta tctttctttt ggcaaatttg aaggctgtgt ttgacgaaat
     5521 tggtgatatt tatcaccgca tttgcccgca catcgaagca tattgcacaa taaaattatt
     5581 atataatgaa atatttcttt ttaccagttg taatgacagc cttattaatt agctgtagta
     5641 attcaacggc cgaaaaacct gctgcagaaa aaacagatac cgttgtttct nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc tcaatttaaa ggaaagaaag
     5821 tctttgtaaa tctttgggcc acatggtgcc ctccctgcag ggcagaaatc ccttctatag
     5881 aaagccttgc atcaaaaaca gacaggtcaa aggttgagtt tgtaatgctt tctttagatg
     5941 acgattttgt aaaagcaaag atttatgcag caaataataa gatgaaactg cctgtttact
     6001 acccgactgg aaacttgccc caattattca atgtggaggg gatacccact acttttatct
     6061 ttaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcaa cggaaataaa
     6181 aaagccacat gattaatgtg gctcttttgt gctacaacca aattcactta ctcttctgtt
     6241 ttcacaggtg ccaataattt ttcttcaatt atctttttga aaatatgttt gggaaatgca
     6301 ccaggctgca tcataggttc accttctgag tcaataaaaa gcagtgtagg aatactttga
     6361 attccaaata cggcagaaag ttcctgctct acatccgtat ctactttata aataaccaag
     6421 cggccttcat actcctttga aagttcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gccggaagtt gtccctggta tttccagtct
     6541 ttctccgtct cgtagttaaa caccttattc ttaaaatcct gagtggtcat ttttattgtt
     6601 gccatatctg ataatttgta caaaagtaac caacttacat gtggtgatgc gtgacgaata
     6661 tcacaaaaaa gccagccatt ataaggctgg ctttcagtat actaagcaat catcagagca
     6721 tggtagtggg ctctacataa tgtgtcttct taagacctgt ctggcttaag gatttgtagc
     6781 ccccctgaat gttgaccaat ttatcaaacc cccttgcttt tagaattgaa cacataatta
     6841 cagaacgata tccnnnnnnn nnnnnnntaa agtatttttt atccctgctc aactgcgaca
     6901 tgttttggtt tatgaaatca agcggaaagt tttctgctcc catcaggtgc tcagaatcaa
     6961 attcactttt acgtctcgca tccagcaggt ttatatgttc ggtatccatc tggtaaaggc
     7021 tggcaaagtc attagcactt atttctttta tagaatctgt ttctaagcct gcttccttcc
     7081 aggctttaat gcccccttgc agaaaaccta ccgcattatc gtaccctact cttgacaaac
     7141 gaaccactac ttcctcttcc cttccttcat ccgccaggaa taatatgggt tgcttcagat
     7201 ccattatcaa agtgcctgac cagggagcga aattgtcatc aataccaata aatattgatc
     7261 cgggaatatg ttcttctaca aaagcttctt tggcccgggt atccaataca agagcttcca
     7321 tactttccca aacggtctga aactctttga cattaagagg tcgtttacct cttgcaatga
     7381 tatcatcaat gctttcaata ccacctttca ggttcattag tacattgtag gggaaatact
     7441 gcggtggttc tactaatcct tctgttactt ctttaatgaa ttcctctttt gacatatcag
     7501 atctaagggc ataattgact tttttctgat gccctaatgt atcagtggtc tctttactca
     7561 tatttttacc acaggcactg ccggcaccat gaccaggata tacaattact tcgtctgcca
     7621 aaggcatgat ctggttcctg agcgaatcat atagataccc tgcaagtatc tgcggagtga
     7681 tctccgcctt cactttttgt actaaatcag gacggcccac atcaccaata aataatgtat
     7741 cgcctgtgaa tatcgcatga tcctttcctt tttcatctat cagcaagtaa gtggtggatt
     7801 ccatggtatg acccggtgta tgtaacactt taatcttgac atctcccaac tgaagtacct
     7861 cgccttccct ggccacatgt acatcatatg ccggtgcagc ggtaggccca aaaacaattg
     7921 ttgcccctgt ttttttagcc aggtcaatat gaccgctgac aaagtcagca tggaaatggg
     7981 tttccagtat gtatttgatc tttgccccgt ttctttctgc cttatcaata taaggtttgg
     8041 tttcccgtaa tgggtcaatt atggcagctt caccgttgct ctcaatataa taggctgctt
     8101 ctgccaggca accggtataa atttgttcta ctttcataat aaaaaatttg attttggtaa
     8161 tgagtaataa tgaaatatcc ttccaacatt aaaagagaaa tgactttttt attaagggca
     8221 acgggtggtg cataagtatt ccattggtct agggtttagt ttataaaatt gattaacaaa
     8281 acaaatttct ctgtggaggg taccgggcaa aatgacaaaa gtcacagaaa atgctgactg
     8341 taatcaggta tagaaataga ctttatatga ttatcgttca aaatgctgag ggtgtgacta
     8401 ttgtcactac atccagctgt aaccctgcac tactttagtg ttctaaattg atttatatgc
     8461 aaaccaaaca tcaaatttta atcgtaggcg gaggtaatgc cggtatctcc accgcttctc
     8521 agctattacg taaaaacaaa aaccttgata ttgctatcat cgaaccatct gacaaacatt
     8581 actatcagcc agcatggaca ctggttggcg c
//
LOCUS       DAORLS010000122         8587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4456_length_8587_cov_1.986873, whole genome shotgun sequence.
ACCESSION   DAORLS010000122 DAORLS010000000
VERSION     DAORLS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4456_length_8587_cov_1.986873"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="PK977_10270"
     CDS             <1..1324
                     /locus_tag="PK977_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF18547.1"
                     /translation="IAGKENPVTVKAYTADNAISSRGIIINEDKTQEKTTTPPNLYAV
                     MVGVSDYKDEELDLKYAAKDANDISAAVAASARKLLNTDGKEHVFVYDLTTSEKRYQL
                     PEKNSIKKVLEEIGNKATANDILLLFFAGHGVMSARSGEQQKFYFLTADASKTTAASA
                     VADVGISTAELTEWMKPQNIRAQKRILIFDACNSGQAIRDLVKVGNERQGYMAARSDE
                     QSQQIKAIDKLNEQSGFFILSASASNQSAYEMGRYSQGLLTYSLLKAIKQQPDILADG
                     KYLDVSRWFNAAEKTVTELTKETGARQQPQVISNTNFTIGIVDEEVMAKIVLPQEKPL
                     FAASNFQNNDENIADDDLELSKLINLQLNDMASRGASSKIVYVTATNSPDAYSLTGRY
                     TINNKVISVQVNLKQNKLIKARFELRGTSDKLNELAAAVALKAAELIK"
     gene            complement(1418..>1676)
                     /locus_tag="PK977_10275"
     CDS             complement(1418..>1676)
                     /locus_tag="PK977_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRF18548.1"
                     /translation="KMSRSKAIASVTDKQFRDELEKHGVKLLGGGLDESPFAYKDIHV
                     VMQSQKALVDIVGKFTPKIVKMDGAAHRSWRKKNKDEMSGE"
     assembly_gap    1677..1698
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1699..>2022)
                     /locus_tag="PK977_10280"
     CDS             complement(<1699..>2022)
                     /locus_tag="PK977_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRF18549.1"
                     /translation="TLDEEEGIEYWKAMNLAGDYASACHHIIHAKIAKQLGRQPLTRV
                     ENHHNFAWKEIYEGKEVIVHRKGATPAGKNVLGIIPGSMTADGFIVKGKGETAAVNSA
                     AHGAG"
     assembly_gap    2023..2032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2033..2893)
                     /locus_tag="PK977_10285"
     CDS             complement(<2033..2893)
                     /locus_tag="PK977_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRF18550.1"
                     /translation="MNKLKITGKELRAIGFPEGPVISIAMNVMQKKYRHHTKEDVMEL
                     LKKILAAPVEYENDAVLGLIATQLLPKKNTEGAEISLNQNGIPFNVFGQEHIEEGAMH
                     QMYQAAKLPVSVAGALMPDAHSGYGLPIGGVLATANAVIPYGVGVDIGCRMCLSIFDI
                     HPKELTSRESFFARELGEAALFGSGAQFDRAADHEVMEHELFYQLPLLKNLHGRAWKQ
                     LGSSGSGNHFVEFGVVEIAEKDEVLGVEAGSYVGLLSHSGSRALGANIANHYTKLAIS
                     KRRLPQEAKNL"
     gene            complement(2965..3102)
                     /locus_tag="PK977_10290"
     CDS             complement(2965..3102)
                     /locus_tag="PK977_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18551.1"
                     /translation="MKFLIAVLLIRFVAELVFRKDNKEEINATAGTSSKVFHPAEYEL
                     R"
     assembly_gap    3373..3502
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4009..>4219
                     /locus_tag="PK977_10295"
     CDS             4009..>4219
                     /locus_tag="PK977_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRF18552.1"
                     /translation="MKPQTGMLLKATAALNDTNFENAVILITEYNEKGAVGFVVNKLF
                     SRQLNELEEFKHSPAFPLYEGGPVDT"
     assembly_gap    4220..4230
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4231..4462
                     /locus_tag="PK977_10300"
     CDS             <4231..4462
                     /locus_tag="PK977_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRF18553.1"
                     /translation="LFFIHQRPDIIQEGKAIGDDVYYGGNFKQAITAINNKSLTVSDI
                     KIFIGYCGWNAGELEAEMAEGSWEPAEEEVFA"
     gene            4560..>5373
                     /locus_tag="PK977_10305"
     CDS             4560..>5373
                     /locus_tag="PK977_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18554.1"
                     /translation="MKKPGSPTASGPISNGPQSKSRRKLLKLLGLGTGLSATPVFAGS
                     TEEWADSLFSTADLLNDKTELVLFRPQDLLELKMTFTGYKKSPDNKSLIKTAAPYLLL
                     VEFHPQSLAEQAWEEAGGEDGQANLDAAAKKDLKWNKPNEPVITSMIIGNPSINPGLK
                     TIIPPAKTYIAGKTRLVFDTKGKDNIVLSPSSLLDWGSFQLVVNKRATCSPVFSAADI
                     NTAPYNNINIKAPQKVDVIKTEPVKTEPVKQPPANNNNPGRRIIQPATKDTLR"
     assembly_gap    5374..5594
                     /estimated_length=221
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5595..8115
                     /locus_tag="PK977_10310"
     CDS             <5595..8115
                     /locus_tag="PK977_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18555.1"
                     /translation="FAAWKQETKLKNRPDLQGTQLSTFELWHSRLASTNCRGALDNTR
                     ATDAIKTVRALWATDIDGDWKNKPERDYSPDGKMDSAGLNKFITSLYNDDRHCIVHQT
                     SNFGITGFRPKPVQVNNLMLSTLGAWLDAIVEFKRPELSSVLSDLNMLKWKHIATLAR
                     DHYVEVVYAGNMYPFGHEAALVRITERKPKNGFAANWQRYFVAITEEEKKYNPVNREN
                     KHNAFPFSTIKIVTAATPTIKTPTKFTGTISGNDDHQFVPTMPNGQPFLFKLVGYDYD
                     GGDVSFEMPLVFVSTNVSVNDSQAFHEGNINKLNNAYNGNAINTVSLRNQKLALARSV
                     NKPGETHYETANIVFNGRFDKNEAPGFVPTTASLDIFASAVENITGQRKAIKVQLEDD
                     RNAGGVFAKLHAASSKLDVNFNGSGNKTGGSLSPNFSITGLSKAYGVISGKLEDAKNK
                     LMDPSQFFNTDAKLFGIIPLGDIIKKTADLLNGESPIPALKNIETEEAHITQYNWKGA
                     RLVERKFFGDVLTFKPGGDARLIIETSLYRYKANKPNALIVDSHISNFDVILMGLAKV
                     GFKKVGFKTGANTKTDFTVDMKNPALEFLGPLTFINVLQKFIPADGFSDPPFLDITTS
                     GITSGYTLALPDVQLGAFTLRNINLGASVSLSFTGGPLIMRFNFCEKHQPFTLTVSAL
                     GGGGFFAMELDMKGVRTIEAALEFGAAASINLGVASGAVSIMGGVYFKLTKKEDDGKE
                     ITIEGYLRINGSLSILGIITASLEFLLQLIANIETVNGKDKVTKVWGQATLKIKIEIF
                     MFSKTVTLKTSREFAGAGADPTFAMLISEGEWQQYCDAFAA"
     gene            8155..>8587
                     /locus_tag="PK977_10315"
     CDS             8155..>8587
                     /locus_tag="PK977_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18556.1"
                     /translation="MSQKIIVTTCPWKDYKTGKWMLSAMMNIQLETAAETTLAAFPDI
                     LKWMEKIQQAVFYVQWNSGTPAELKPVTSKWDPALYEKLFHSGIKVKGFAPVNMATIK
                     IKSYPVKHISNFIFDTYMEVGNLKMTELPKASFFTKEYTRLN"
BASE COUNT         2322 a         1837 c         1837 g         2197 t
ORIGIN      
        1 tatcgcaggt aaagaaaacc cggttacggt aaaagcctat acggccgata atgctatttc
       61 atccaggggc atcattatca atgaagataa gacacaggaa aagacaacaa ctccacccaa
      121 cctctatgct gtaatggtgg gagtaagtga ttataaagat gaagagctgg atttaaaata
      181 tgcggccaaa gatgccaatg atatatccgc tgccgtggct gcttcagccc gtaaactgct
      241 gaataccgat ggcaaagaac atgtttttgt atatgatctt actacttcag agaaaaggta
      301 ccagttgccg gaaaagaaca gtatcaaaaa agtgctggaa gaaattggca ataaggcaac
      361 agccaatgac attttgttat tgttttttgc cggtcatggg gtgatgtccg cccgttccgg
      421 agagcaacaa aaattttatt tcttaacggc cgatgcttcc aaaaccacgg ctgcttctgc
      481 tgtggccgat gtgggcatca gtaccgccga gctcaccgaa tggatgaagc cgcaaaacat
      541 cagagcacaa aaacgcatcc tcatctttga tgcctgcaac agcggccagg ccatccgtga
      601 cctggtaaaa gtgggtaatg aacgacaggg ttatatggca gcccgcagtg atgaacaaag
      661 ccagcagata aaagctatag ataaactgaa tgagcaatct ggctttttca ttctttcggc
      721 atcggccagc aaccagagtg cttatgagat gggcaggtat tcgcaaggcc tgcttactta
      781 ctccttatta aaagcgatca agcagcagcc ggatatttta gcagacggaa aatacctgga
      841 tgtaagccgc tggtttaatg ccgctgaaaa aactgttact gaacttacca aggaaaccgg
      901 tgccagacag cagccgcagg tgatcagcaa taccaatttt actatcggca ttgtagatga
      961 agaagtaatg gccaagattg tgctgccaca ggaaaagcca ttgtttgctg ccagtaattt
     1021 tcagaataat gatgagaata ttgcagatga tgatttggaa ttaagcaaat tgattaacct
     1081 gcaattaaac gatatggctt ccaggggagc tagcagcaag atcgtttatg taacggctac
     1141 caattcaccc gatgcctatt cgctgaccgg ccgctatact atcaataata aagttatttc
     1201 ggtgcaggta aacctgaaac agaacaagct gatcaaagcc cgttttgaac tgaggggtac
     1261 aagcgataaa cttaatgaac tggctgcagc agttgcttta aaggcggcag agttgataaa
     1321 atgaaatgac aaatcatgac ctcatggagg agtgtttgaa agaggggccc ggtgttgaag
     1381 tttgccaggc cctttcttcc ataatcagat gatgcgttta ttcaccgctc atttcatcct
     1441 tgtttttctt tctccagctt ctatgtgcgg cgccgtccat cttcacaatc ttcggcgtga
     1501 actttccgac tatatccacc aatgcttttt gtgactgcat cactacatgg atgtctttgt
     1561 aggcaaaagg tgattcatcc agtccgccgc caagcagttt aacgccatgc ttttccagtt
     1621 catccctgaa ctgtttatca gttaccgatg caatagcttt agaccggctc atttttnnnn
     1681 nnnnnnnnnn nnnnnnnnct tcctgcgccg tgtgcagcag aattgacagc agctgtttcc
     1741 cctttgcctt tcacaataaa tccgtcggct gtcatggaac ccggaatgat acccagcaca
     1801 tttttgccgg caggggtggc acctttccgg tgaacgatta cttcctttcc ctcatagatt
     1861 tccttccatg caaaattgtg gtgattctcc accctggtca aaggttgacg acccaattgc
     1921 tttgcgatct tggcatgtat gatatggtga caggcggatg cataatctcc cgcaaggttc
     1981 atggccttcc agtattcgat gccttcttct tcatccaatg tcnnnnnnnn nnaaggttct
     2041 ttgcttcctg cggtaacctt cttttgctga tggccagttt ggtgtagtga ttggcaatat
     2101 tggcacccaa agcccttgaa ccggagtgag acaataaacc cacatagctg cctgcttcaa
     2161 cgcccagcac ttcatctttc tcagcgatct ctactactcc aaactccaca aagtgattac
     2221 ctgaaccgga agaacctaac tgtttccatg cacggccatg caggtttttc aacaagggta
     2281 actgataaaa caactcatgc tccatcactt catgatctgc cgcccggtca aactgggcac
     2341 cgcttccaaa caaggcagct tcacctaatt ctcttgcaaa aaaagattcc ctgctggtca
     2401 gctcttttgg atggatatca aaaatgctca ggcacatcct gcagccaatg tccaccccca
     2461 caccataagg aataacggca ttggctgtag ccagtacacc accaatcggc aatccatagc
     2521 cgctgtgcgc atcgggcatt aatgccccgg ccacactgac cggcagttta gctgcctggt
     2581 acatctggtg catggcgcct tcttcaatat gttcctgtcc gaaaacattg aagggaattc
     2641 cattttgatt caatgaaatc tctgcccctt ctgtattttt cttcggcaat aattgtgttg
     2701 cgatcaatcc caacaccgca tcattttcat attccaccgg cgctgccaat attttcttca
     2761 atagctccat cacatcctcc tttgtatgat gcctgtattt tttttgcatc acattcatgg
     2821 ctatgctgat caccggtcct tccgggaagc cgatagccct tagttctttt cctgttattt
     2881 ttaatttgtt catatctgtc ccatacgggt gtttaaagtt catcaatttt agtcagccgg
     2941 ataagcaccc ggctgacgtt ttgattatct taattcatat tctgccgggt gaaacacttt
     3001 gctgctggtt ccggcagttg cgttgatttc ttctttgtta tcttttctga ataccagttc
     3061 cgcaacaaaa cggatcagta atacagcgat taaaaatttc atagttacct ccttttttac
     3121 cggctattaa ccgccgccag ggcttgcaac cgctgacggg gttagggtat aaaaaaaccc
     3181 ggtcgtttag agcgaccggg ttttccttca caggagtagt tactcctatg tatggacatg
     3241 atccggccgc agataccttt tatctttttc attgccaatg ggggctgcac ttggtagtaa
     3301 ctgcgcatac ccatagcacc tgcgtgataa acatgtatca ttcatactcc atgtaaatcg
     3361 tttatgtttt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnaaaaaccc cgacgattct gccggggcct gtattgattg
     3541 aattcgttat tacacttatt cagcagcact acatgccccg caacctgcat agtctccttt
     3601 aggactaaac ctgttcactg attcaatatg tgtatttctg cgaagcattt tttatttttt
     3661 gcgaatgcaa aggtgaaact atttttttga tgttgccaaa ttttatcaat tatttttttt
     3721 gtttgtcatg cagtttgcag gtcgatgatt gctatttcat ggaatgagct gatgatttag
     3781 tgtggttgaa tatagaggaa ggttgttgcc cccgacaatc ccgaaaacta tcgggacagg
     3841 gtgacagcgg tttagttact tgtctttcaa caataccaat aactatcagg acagggtagc
     3901 ggggctataa aaaagtcatc acaatactgc ctgaaatgag tttcatcatt tccttccgca
     3961 acggtacggc gtaacttcat gttacaatct tcacttaaag caattgacat gaaaccgcaa
     4021 actggtatgt tgctcaaggc tactgcagca ttgaacgata ctaattttga aaatgcagtt
     4081 atactgatca cagaatataa tgagaaagga gcagttggat ttgttgtcaa caaactcttt
     4141 agccgccaac tgaatgagct ggaagaattt aaacacagtc ctgcttttcc gttgtatgaa
     4201 ggagggccgg ttgatactgn nnnnnnnnnn tttgtttttt atccaccaaa ggcctgatat
     4261 aatacaggaa ggaaaggcta ttggggatga tgtttattac ggcggcaatt ttaaacaggc
     4321 cattacggca atcaacaata aaagcctcac cgtatcggat attaagatct ttatcggcta
     4381 ctgcggctgg aatgccgggg agctcgaagc agaaatggcc gaaggaagct gggagccggc
     4441 agaagaagaa gtgtttgcat aaaggctgag ccaaaaaagt tattgacaat tttatttttt
     4501 tcagttggaa ttgcaggtaa aaaagttaag tttggttaat aaccatcaca cctttcttta
     4561 tgaaaaaacc tggcagccca actgcaagtg gtcccatttc caacggacca caaagtaaat
     4621 cccgccgtaa gcttctaaaa ttactgggac ttggcaccgg tctcagtgct actcctgttt
     4681 ttgcgggcag cacagaagag tgggctgatt ctttattcag cactgctgat ttactcaatg
     4741 acaaaaccga gctggtactc tttcgtccgc aggacctgct ggaactgaag atgaccttta
     4801 ccgggtataa aaaatcacca gacaataagt cattgatcaa aacagcggcg ccttacctgc
     4861 tgctggtgga gtttcatccg caaagtcttg ctgaacaggc atgggaagaa gcaggtggtg
     4921 aagatggcca ggctaactta gatgcagccg ccaaaaagga cctgaaatgg aataagccta
     4981 acgaacctgt gatcacttca atgatcattg gtaatccctc tatcaatccc gggctgaaga
     5041 ctataattcc cccggctaaa acttatatag ccggtaaaac aagattagtg tttgacacaa
     5101 aaggaaaaga caatattgtt ctttctcctt cgtcattact ggactggggc agttttcagt
     5161 tagtggtcaa caaacgggcc acttgctcac cggtattcag tgcggctgat attaatacag
     5221 cgccttataa taatatcaat atcaaagcgc cgcagaaggt tgatgtaata aaaacagagc
     5281 cggtaaaaac ggaaccggta aagcagccgc cggcgaacaa taataatccc ggccgcagga
     5341 taatacaacc ggccacaaaa gatacgcttc gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnngtttgc tgcctggaaa caggaaacga aactgaagaa ccgtcccgac
     5641 ctgcagggca cacaactttc tacatttgaa ttgtggcatt caagactggc cagcaccaat
     5701 tgccgaggag cacttgataa cacaagggct accgatgcca ttaaaacggt gagggcatta
     5761 tgggctactg atattgacgg tgactggaaa aacaaaccgg aacgggatta ttcgccggat
     5821 ggcaaaatgg attctgccgg gctgaataag tttatcactt ctctctataa tgacgaccgt
     5881 cactgtattg tacaccaaac cagcaatttt ggtatcaccg gattcaggcc taagccggtg
     5941 caggtaaata acctgatgct gagtacactt ggcgcctggc tggatgctat tgtcgaattt
     6001 aaaagacccg aactaagcag tgtgctcagc gacctgaaca tgctgaagtg gaaacatatt
     6061 gccacattgg caagagatca ttatgtggaa gtggtgtatg ccggcaacat gtatcctttc
     6121 ggtcatgaag cagcattagt acgtattaca gaacgcaaac cgaagaacgg gtttgcggct
     6181 aactggcagc gttattttgt ggcgattaca gaagaggaaa aaaaatataa ccctgtcaac
     6241 cgggagaaca agcacaatgc ttttcctttt tccaccataa agattgtaac ggcagctacg
     6301 cctactatta aaactccgac aaaatttacc ggtaccatca gtggtaatga tgaccaccag
     6361 tttgtgccca ctatgccaaa cggccagccc tttcttttca aattagtggg ttatgattat
     6421 gatggtggtg atgttagttt tgaaatgccc ctcgtatttg tcagtaccaa tgtgtcggtc
     6481 aatgacagcc aggcatttca tgaaggcaat atcaacaaac tgaataatgc ttacaatggc
     6541 aatgccatca ataccgtttc gctacgcaac cagaaactgg cccttgcccg cagcgtaaac
     6601 aaaccgggcg aaacacatta tgaaacagcg aacattgtct ttaacggccg ctttgataaa
     6661 aatgaagcgc cgggttttgt cccgactact gcttcgctgg atattttcgc cagtgccgtg
     6721 gagaatatca cggggcagcg taaggctatc aaagtgcaat tggaagatga ccgcaatgcc
     6781 ggtggtgtat ttgcgaaact gcatgctgcc agctctaaac tggatgtaaa ttttaacggc
     6841 agcggtaata aaacgggggg cagtctttcg cccaattttt ctattaccgg tttatcaaaa
     6901 gcatacggcg ttatcagtgg taagcttgaa gatgcgaaga acaaactgat ggacccgtcg
     6961 cagttcttta atacagatgc caaactattt ggcattatac ccctcggtga tattattaag
     7021 aaaacggctg acctgttaaa tggcgaatcg cccattcctg cattaaagaa tatagaaaca
     7081 gaagaagcac atattactca atacaactgg aaaggtgcca ggctggttga acgaaagttt
     7141 tttggtgatg tgctcacttt caaacccggt ggtgatgccc gcctgatcat tgaaacaagc
     7201 ctttaccggt acaaggccaa taagccgaat gcgctgattg tggattcgca tatcagcaat
     7261 tttgatgtaa tcctgatggg gcttgccaaa gtgggcttta agaaagtagg gtttaaaacc
     7321 ggcgctaata cgaagacgga ttttacagta gacatgaaga acccggcgct ggagttttta
     7381 gggccgctca ccttcatcaa tgtattgcag aagtttattc cggcagatgg tttcagtgat
     7441 ccgccattct tagatataac aaccagcggc attacttctg gttatacact ggccttaccg
     7501 gatgtgcagc ttggcgcttt cactttgcgg aatatcaatc tcggtgcatc ggtgagttta
     7561 tcctttaccg gcggcccgct gatcatgcgg tttaatttct gtgaaaagca tcagcccttt
     7621 acactgactg tttctgcatt gggtggaggt ggtttctttg cgatggagct ggacatgaag
     7681 ggtgttcgca ccattgaagc agcgctggag tttggcgctg ccgcctctat caatctcgga
     7741 gtagccagtg gtgccgtaag tattatgggt ggtgtatact ttaaactcac caaaaaagaa
     7801 gatgatggca aggaaataac gatcgaaggt tatttgcgta tcaacggctc attaagcatt
     7861 ttgggaatca tcacggccag cctggaattc ctgctgcagc ttattgcgaa tattgaaact
     7921 gtaaacggta aggataaagt aacgaaagtg tggggccagg caacattgaa gatcaagatc
     7981 gagatattca tgttcagcaa aaccgttacg ctgaagacct cgagagagtt tgccggcgcc
     8041 ggtgccgatc ccacatttgc catgcttatc agcgaaggag aatggcagca gtattgtgat
     8101 gcattcgccg cttaaaacca gtaaccagca accagaaaca agaaacttta gcatatgtct
     8161 caaaaaatca ttgtcaccac ctgtccgtgg aaagattaca aaacaggtaa atggatgctc
     8221 agcgccatga tgaatattca gctggaaaca gcagcagaaa ccacattggc agcttttccc
     8281 gatatactaa agtggatgga aaaaattcag caggctgttt tctatgtgca atggaatagt
     8341 ggtacacctg ccgaattaaa accggtaaca tcgaagtggg atccggcctt gtatgagaaa
     8401 ttatttcaca gcggtattaa agtaaaagga tttgcaccgg tgaacatggc tacgatcaag
     8461 atcaaatcat acccggtaaa gcatatcagc aattttattt ttgatactta tatggaagtg
     8521 ggtaacctga aaatgacaga gctgcctaag gccagtttct ttaccaaaga atacaccagg
     8581 ctgaacc
//
LOCUS       DAORLS010000123         8492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4515_length_8492_cov_1.721821, whole genome shotgun sequence.
ACCESSION   DAORLS010000123 DAORLS010000000
VERSION     DAORLS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4515_length_8492_cov_1.721821"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK977_10320"
     CDS             complement(<1..264)
                     /locus_tag="PK977_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18557.1"
                     /translation="MKPTILLLSIVTLALSISVKAQFRKYSNEFLNIGAGARGLSMGS
                     AQVASVNDGTAGYWNPAGLVGINEYPQVNLMHAEYFAGIGKYDY"
     assembly_gap    421..498
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <499..>837
                     /gene="tyrS"
                     /locus_tag="PK977_10325"
     CDS             <499..>837
                     /gene="tyrS"
                     /locus_tag="PK977_10325"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRF18558.1"
                     /translation="EELRWRGMIQHIMPGTEEQLNKEMTTGYIGFDPTADSLHIGSLV
                     PILLLVHFQKAGHKPIALVGGATGMVGDPSGKSEERNLLSEETLQKNVAGVKAQLEKF
                     LDFNPTLPNAA"
     assembly_gap    838..850
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <851..1785
                     /gene="tyrS"
                     /locus_tag="PK977_10330"
     CDS             <851..1785
                     /gene="tyrS"
                     /locus_tag="PK977_10330"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRF18559.1"
                     /translation="AAVMANNYDWFKTISFIDFLRDAGKHITVNYMMAKDSVKKRIEG
                     ETGISYTEFAYQLMQGYDFYWLYNYKNCKLQMGGSDQWGNMTTGTELIRRKCGGEAFV
                     FTNPLITKADGGKFGKTESGNVWLDANRTTPYQFYQFWLNASDTDAEKWIKIFTFLSR
                     DAIEMLIHEHKMDPSARVLQKTLAQEITCFVHGKEEFEMAVETTQKLFANQNAPAESL
                     SIDDLEGMEGVVKSDYSKEKISAGIDVVSFLAETEILSSKGEARKLVQAGGISINRKK
                     VNAVDFKIESSLLLHDTYILVQKGKKNYYLVKAV"
     assembly_gap    1936..2033
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2034..3780)
                     /locus_tag="PK977_10335"
     CDS             complement(<2034..3780)
                     /locus_tag="PK977_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF18560.1"
                     /translation="MKKKQIVFVVLLVSLAFSFRKPVKEQNRYIPPAPSVEVVEMQPS
                     DARGNLYYMVCAAKTSADKPTAQLSVKFTIKNKEGKKLVLKTIAYSFLQKGSPVSKTM
                     VMDIDDNAVKDFSIAAGAQFSWQNSRDYHDVDNAVFFDNPFPASLQIKLTFEGYSTPV
                     IITRNLRGYDNAAPGGAYGFPGRLSHLKEGEYWYAYGGHGGGGQFYAYDFKVEGWDPK
                     AKKWSNTFPGKDGTKNEHYRAYGKAVIAAADGVVIDFKDGVKENEGNGGGGSGGGNWF
                     KINNGKETLCYYHMQPGTLTTAFLKKGAKVKKGDKLGLLGNSGNSSEPHGHVHAITDP
                     DADGEGQWVPMNFSDIYIIDKNELGAGPDPDASWTKVEKKGLPFLQDRRCMIWPSATK
                     PCLYPGGWAEIGRHAIPENQYQQEITRIWGCGYYPVWVDAYDVSGKTYFNTIFRYNSG
                     NYPVVVRHDMTKESYQQEYNTWIKQKDYRLLQLDNYNDNGKLKFAAIFIKKQNQPAAQ
                     PAYHAQSPEEHQQLFETYTGQGYVPVNVSVVSVGGKKYYSAFYEKRNVGGSVLKSSLS
                     QQEYQDMFEDMKQKKW"
     gene            complement(3918..4592)
                     /locus_tag="PK977_10340"
     CDS             complement(3918..4592)
                     /locus_tag="PK977_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRF18561.1"
                     /translation="MKNLLVISVVLLISCAFTAQKKTKVIFFGDSITELGVKKDVGYI
                     VKMDSMLKAEKKDDQYELIGSGISGNKVYDLYLRLEEDVLVKNPDVVVIYIGVNDVWH
                     KTLLGTGTDADKFEKFYQAIINKLSAKGIKAILCTPAVVGEKTDMSNQLDGDLNRYSN
                     IIRDLAKKNNLPLVDLRKKFLDYLKDNNPRNEEKNILTYDRVHMNDKGNQFIADQMWK
                     EIKDIK"
     gene            complement(4654..5550)
                     /locus_tag="PK977_10345"
     CDS             complement(4654..5550)
                     /locus_tag="PK977_10345"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRF18562.1"
                     /translation="MSNPKGKLIAIGGAEDKGTDLEIGEIHRNNLNFFELGILRRVVE
                     EAGGVNSRIEVITTASTIPYEVGENYLNAFGKIGCTNIGLLHIRNRQDAMNDEYIERI
                     RHCDAVMFSGGNQLRLSVTDGGTEFLAILKKRYQEEEQFLIAGTSAGAMAMSNTMIYE
                     GNATRAHLKGEVKITTGLGFINSVIIDSHFEKRGRFGRLAQAVAANPGCIGIGLGEDT
                     GMLITEGNKMEAIGSGLVVIVDGHEIMHSNIADIPEGNPISIENLKVHFCEKGNGYLI
                     RERKYLPEASSGSVIPKQVDVE"
     assembly_gap    5609..5812
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5813..>6072)
                     /locus_tag="PK977_10350"
     CDS             complement(<5813..>6072)
                     /locus_tag="PK977_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18563.1"
                     /translation="ATSDNPLKDDIRKFEALGTRPFYIEQAIGKAIEFHDMIGIERKQ
                     KRLHYLKNYWMEKVRDIHGVKLHTSFDAKWGCAIGNVGIAGK"
     assembly_gap    6073..6153
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6154..>6763)
                     /locus_tag="PK977_10355"
     CDS             complement(<6154..>6763)
                     /locus_tag="PK977_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF18564.1"
                     /translation="VSPALINLNNGGVSPAPKTVQDAMKRYYDLSNEAPSYYMWRILD
                     QGREPLRKNLARLAGCSPEEIAINRNSSEGLETIIFGLQLKAGDEVVAAKQDYPNMIN
                     AYKQRELRDGIKMAWINLDLPSEDEDYLVRKYVNAFTPKTKLVHITHIINWNGQILPV
                     KKIAAEAHKRGIEVVVDGAHSFAHFDFKIPDLDCDYFAASLH"
     assembly_gap    6764..6918
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7103..>8492
                     /locus_tag="PK977_10360"
     CDS             7103..>8492
                     /locus_tag="PK977_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF18565.1"
                     /translation="MKILEIKVLKGPNYWSVRRNKLIQMKLDLEEKENMPTNKIPGFR
                     ERLEKLLPSLYEHRCSVGKPGGFFQRVDEGTWMGHVVEHIALEIQTLAGMDTGFGRTR
                     TPEGEKEGIYYVVFSYMEEDAGVYAAKAAVRIAQALIDGQEYDMTDDIQKMREIREDT
                     RLGPSTGCIVDEAAKRGIPYIRLNKQSLVQLGYGVNQKRIRATIASTTSNIAVDIACD
                     KEETKMLLEAAEIPVPRGTVIRTEVGLDEAIEKFGYPLVIKPIDGNHGKGNTTNITNK
                     EQALKAFAAAKEYSRSVIVERFITGYDFRCLVINYKFICAALRTPASVVGDGIHNIQW
                     LIDETNKDPRRGYGHEKVLTQITVDQFTQKMLDDKGYTLETVPVKGERVLLKPTANLS
                     TGGTSTDVTDEVHPANIFMFERIAKIIGLDICGIDVMANDLRTPINENGGAILEVNAA
                     PGFRMHIEPAEGL"
BASE COUNT         2342 a         1752 c         1521 g         2248 t
ORIGIN      
        1 atagtcgtac tttccaattc ccgcaaaata ctctgcatgc atcaggttta cctgtgggta
       61 ttcattaata cccacaagtc cggcaggatt ccaataaccg gcagtaccat cgtttacaga
      121 agctacctgg gcacttccca tagataaccc cctggcgcct gcacctatat ttaaaaattc
      181 attggaatac ttcctgaatt gggccttaac tgaaatggac aaggctaatg tcacaatgga
      241 caatagaagg atggttggtt tcattcggac aattttcgat aagcgtttga attagacaag
      301 ctttcatacg tacgggctgc ttgtaaaatt agtctttaag cctgtaaaat catagttccg
      361 ttgagcaaaa agactattta ttgaaaacga acggtttaga aaaatattgc ctgcattatc
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnncg aagaattaag atggagaggt atgatccagc acatcatgcc
      541 aggtaccgaa gaacagttaa acaaagaaat gactaccggc tatatcggct tcgaccccac
      601 agccgacagc cttcatatag gcagtttagt cccaattctg ttactggtac attttcaaaa
      661 ggcaggtcat aaacctattg ccctggtcgg cggagctacc gggatggtgg gtgaccccag
      721 tggcaaaagt gaggaaagaa atcttttaag cgaagaaaca ttgcaaaaaa acgtagcagg
      781 agttaaagca caattggaaa aatttcttga ttttaatcca actcttccga atgctgcnnn
      841 nnnnnnnnnn atgctgctgt aatggctaat aactatgatt ggttcaaaac catttcattt
      901 attgattttc tgagagatgc cggcaaacat attaccgtca attatatgat ggcgaaagac
      961 agcgtaaaaa agagaattga aggggaaacc ggtatcagct atactgaatt tgcttaccag
     1021 ctaatgcagg gatacgactt ttactggctt tacaactata aaaactgcaa actgcaaatg
     1081 ggcggcagcg atcaatgggg caatatgacc acaggtacag aactgatacg gagaaaatgt
     1141 ggtggggaag catttgtatt cactaacccc cttattacta aagcagatgg cggaaaattt
     1201 ggtaaaactg aaagcggcaa tgtatggcta gatgccaaca gaacaacacc ttatcagttt
     1261 taccagttct ggttaaacgc aagtgatact gatgctgaga aatggatcaa aatattcacc
     1321 tttctatcaa gagatgccat agaaatgctg atacatgaac acaaaatgga tccttcggca
     1381 agggtgctac agaaaacact ggcacaggaa ataacctgct ttgttcatgg caaagaagaa
     1441 tttgaaatgg ctgttgaaac gacccaaaaa ctatttgcca atcaaaatgc acctgccgaa
     1501 agcctgagta ttgacgacct ggaaggaatg gaaggagtag taaagtctga ttattcaaaa
     1561 gaaaaaattt ctgcaggtat tgatgttgtc tcatttcttg cagaaactga aatactctcc
     1621 agcaaaggag aagccagaaa attggttcag gcaggaggca tcagcataaa caggaaaaaa
     1681 gtaaatgctg ttgattttaa aattgaatct tcgctactat tgcacgacac ttacattctt
     1741 gtacaaaaag gaaagaagaa ttactacctg gtgaaagcag tatagtaatc cttcttcaat
     1801 attatcatca tttagaccaa tgcccggcaa accaatgttt gccgccttcc tcataacctg
     1861 ttacgcaacg ggtaagataa ccagcttcca ggctgctttc atatttatcc tggtaaccgt
     1921 ctttgtctga ctttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccattt
     2041 cttttgtttc atatcctcaa acatatcctg gtattcttgc tgagataaag aacttttcag
     2101 cacactaccc ccaacatttc gcttttcgta gaatgcagaa taatatttct tgcctccaac
     2161 agaaacaacc gaaacattca ccgggacata gccctggccc gtatatgttt caaatagctg
     2221 ctggtgctcc tcaggtgact gagcatgata ggcaggctgc gcagcaggct gattttgctt
     2281 cttgataaaa attgcagcaa atttcagctt cccgttatca ttgtaattat ccaactgcag
     2341 caagcgataa tctttttgct ttatccaggt attatattcc tgctggtaac tttcttttgt
     2401 catatcatgt cgaactacga cagggtaatt gccggagtta tacctgaaaa tggtattgaa
     2461 ataagtttta cctgaaacat catacgcatc tacccaaacc gggtaatagc cacatcccca
     2521 tatccttgta atttcctgct ggtactgatt ttcgggtata gcatgtcttc cgatttccgc
     2581 ccaacctccc ggatataggc aaggttttgt agcagaaggc catatcatac aacggcgatc
     2641 ttgcagaaat ggcaagcctt tcttttccac ttttgtccag gacgcatccg gatcaggacc
     2701 tgcacccagc tcatttttat ctattatata gatatcagaa aaattcatcg gcacccattg
     2761 gccttcacca tccgcatcag ggtcagtaat agcatgtaca tgaccatgcg gttcgctgga
     2821 attaccggag ttacccaaca accccaattt atctcctttt ttaacttttg cacctttctt
     2881 taaaaaagct gttgttaaag tgccaggctg catatgatag taacaaagtg tttctttacc
     2941 attgtttatt ttaaaccagt taccgccacc actgcccccg cctccgttac cttcattttc
     3001 tttaacccca tccttaaaat caatcactac accatcggct gcagcaatta ccgctttccc
     3061 ataggccctg taatgttcat tctttgttcc gtctttaccg ggaaaagtgt ttgaccattt
     3121 ctttgctttg gggtcccatc cttccacttt aaaatcatat gcataaaact gacctcctcc
     3181 accatgaccg ccataggcat accaatattc cccttctttc agatgtgaca agcgaccagg
     3241 aaagccataa gcaccgccgg gagctgcatt gtcgtatcct ctgagattcc ttgtaattat
     3301 aaccggggta ctatatcctt caaaagtcag tttgatttgg agactggcag gaaaagggtt
     3361 gtcaaagaaa acagcattgt ctacatcgtg ataatcacgg ctattctgcc agctaaattg
     3421 tgcacctgca gcaatcgaaa aatctttaac tgcattgtca tcaatatcca taaccatcgt
     3481 ttttgaaacc gggctaccct tttgtaaaaa ggaataagca attgtcttta ataccagttt
     3541 ttttccttct ttgttcttta ttgtgaattt gactgaaagc tgggccgttg gtttgtctgc
     3601 cgaagttttg gccgcacaaa ccatataata caggtttccg cgggcatctg aaggttgcat
     3661 ttcaaccacc tctaccgaag gcgccggagg aatatatctg ttttgttcct tgaccggttt
     3721 tctaaaggaa aaagccaaag aaaccagcaa aaccacaaat acaatttgct tctttttcat
     3781 gtcttaaagg tttaatgatg aatttagccg taagctaccc acctaaaact ttaaaaaatg
     3841 agattgctga gtttgcggtc agattgaatt attctccggt aaaagaaata aagagaggtt
     3901 ggttaatggg gaagtgatta tttgatatcc tttatctctt tccacatctg gtctgctatg
     3961 aactggttgc ctttatcatt catatgcacc ctgtcgtaag taaggatatt tttctcctca
     4021 tttctgggat tgttatcctt caggtaatca agaaatttct tacgaaggtc aacaaggggt
     4081 aagttattct tctttgccag atcacggatg atattgctgt atcggttcaa atctccgtca
     4141 agttggttgc tcatgtcagt tttttcaccc actactgccg gagtgcacag gatggccttt
     4201 atgccttttg cagaaagttt attgataata gcctggtaaa atttttcaaa tttatcagcg
     4261 tcagtacctg tgcccagcaa agtcttatgc cacacatcat tcacaccaat atagatcacc
     4321 accacatccg ggtttttaac cagaacatct tcttccaatc gcagatacag gtcatacact
     4381 ttgttgccac tgataccaga tccgatcaac tcatactggt catccttttt ttcagctttc
     4441 agcatgctgt ccatttttac aatgtagcca acatcctttt tcactcctaa ctcagtaata
     4501 gaatcaccaa agaaaataac ttttgttttc ttttgagcag taaacgcaca ggagattagc
     4561 agaacaactg aaatgaccaa taaatttttc atattaataa ttttcaaacg taagagatga
     4621 aacaaataat aaaaaagttg ctgagcatca ttgctactcc acatcaacct gcttgggaat
     4681 aactgatccg ctgctggcct caggcaagta ctttctttcc ctgatcaggt aaccgttacc
     4741 tttttcgcaa aaatgcactt tcaggttttc aatgctaatg ggattcccct ccggtatatc
     4801 agcaatattg gaatgcatga tttcatgtcc atctacaatt accactaacc cgctacctat
     4861 ggcttccatt ttattcccct ctgtaatcag catacccgta tcctctccca atccgattcc
     4921 gatgcaaccg ggatttgccg ccacggcctg cgccaaacgg ccgaaacggc ctctcttttc
     4981 aaaatgagag tcaattatca cagaattaat aaatccaagg ccagtagtaa tttttacttc
     5041 accttttaaa tgggcccttg tggcattacc ctcataaatc attgtgttac tcattgccat
     5101 agcaccggca gaagtacctg cgattaaaaa ttgttcttcc tcctggtatc gcttcttgag
     5161 tatggcaagg aactcagtgc ccccatctgt tacacttagc cggagctggt taccacctga
     5221 aaacattact gcatcacaat gacgaatcct ttcgatgtat tcatcattca tggcatcctg
     5281 ccggttgcgg atgtgcagga gcccaatatt tgtacaaccg attttaccaa atgcattcag
     5341 gtaattctct cctacttcat atggaatagt agatgctgta gtaattacct caatacggga
     5401 attaacccct ccggcctctt ctactactct acgaagtatg cctaattcaa aaaaattcag
     5461 attattgcgg tgaatctcac ctatctcaag gtctgttccc ttgtcttcag ctccgccaat
     5521 agcgatcagt tttcctttag gattactcat tcaagagggt ttgattaaga caaaataaat
     5581 gaaaaaaaac agaacgtaaa ttctcccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttgccc
     5821 gctataccca cattgccaat agcacatccc catttagcat cgaatgatgt atgcaatttg
     5881 acaccatgga tgtctctgac tttttccatc caataatttt taaggtagtg cagtcgtttt
     5941 tgttttcgct caatcccgat catatcgtga aattcgatgg ctttgccaat ggcctgctca
     6001 atataaaaag gtcttgtccc caacgcttca aatttgcgta tatcatcctt caacgggtta
     6061 tcacttgtag cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttatgca ggctggctgc aaaatagtcg
     6181 caatcaagat caggtatttt aaaatcaaaa tgagcaaaac tatgtgcacc gtccaccacc
     6241 acttctattc ctcttttatg tgcctcagct gcaatctttt ttacaggtaa tatctgtccg
     6301 ttccagttga taatatgtgt gatatgaacc agttttgtct ttggtgtaaa agcatttacg
     6361 tacttcctga caaggtaatc ttcatcttca cttggcaggt ccagattgat ccaggccatt
     6421 ttaataccat ccctaagctc ccgctgttta tatgcattga tcatattggg ataatcctgc
     6481 ttagctgcca ccacttcatc accagccttt aactgtaagc caaaaatgat cgtctccaaa
     6541 ccttcagaag aattcctgtt tattgcaatc tcttccggcg agcagccagc caacctagcc
     6601 aggtttttcc ttaagggttc ccttccctgg tccaaaatac gccacatgta atagctcggc
     6661 gcttcattac taagatcata atatcgcttc atcgcatcct gaacagtttt aggggcaggg
     6721 gaaactcctc cattattcag gttgatcaat gcaggggaaa cagnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnag aaatctgcga cggttgcttg aagacatgtc agttatttta
     6961 ataattaaat gtactgaaaa cgttccctta aactgtaaaa gactctaaat gaaaaaggag
     7021 gttaatattg aatcaggcct cccaagtaca agtattggtc accttatgaa aaaaaactat
     7081 cttccaaact caactattgt gtatgaaaat acttgagatc aaagtgctga aaggtcccaa
     7141 ctactggagt gtaaggcgta acaaacttat ccagatgaag ctggaccttg aagagaaaga
     7201 gaatatgcct accaataaga ttccgggttt ccgggaaaga ttggaaaaac tcctgccttc
     7261 cttatatgag catcgttgca gtgtcggtaa acccggcggt ttttttcaac gggtggatga
     7321 aggaacctgg atgggccatg ttgtagaaca tattgcctta gagattcaga cccttgccgg
     7381 gatggatacc ggtttcggca gaacccggac acctgaagga gaaaaagaag gtatctatta
     7441 tgttgtattc agctatatgg aagaggatgc cggtgtatat gcagccaaag cagctgtacg
     7501 catagcccag gcattgatcg atggacagga atacgacatg acagatgata tccaaaaaat
     7561 gagagagata agagaagaca cccgtctcgg cccatccacc ggctgtattg tagatgaagc
     7621 ggccaagaga ggaattccct atatcaggct taacaaacaa agtcttgttc aattgggata
     7681 tggagtcaat caaaaaagaa taagagcaac cattgcctcc accacttcta atatagctgt
     7741 agacattgcc tgtgataaag aagaaaccaa aatgctgctg gaggctgctg aaattcccgt
     7801 gccaaggggc actgtaattc gaacagaagt tggtcttgat gaagcgattg aaaaatttgg
     7861 ctatccactg gttataaaac ctattgatgg aaaccatggt aagggtaata ccactaatat
     7921 caccaataag gaacaggcgt taaaagcttt cgctgccgca aaagaataca gccggagcgt
     7981 cattgtggaa cgattcatta ccggctatga tttccgttgt cttgttatca actataaatt
     8041 catttgtgca gcactacgaa caccagcttc tgttgttgga gatggcatac acaacattca
     8101 atggctgatt gatgagacca acaaggaccc ccgccgtggc tatggacatg aaaaagtgct
     8161 gacgcaaata accgttgacc agtttacgca aaaaatgctg gacgacaaag gttatacatt
     8221 ggaaacagtg cctgtaaaag gagaaagggt tttattaaag ccaactgcca acctctctac
     8281 cggaggaacc tctacggatg ttactgatga agtgcatccc gcaaatattt tcatgtttga
     8341 aagaatagcc aaaatcatcg gattggatat ttgcggaatt gatgtgatgg ccaatgatct
     8401 gcgaacaccc attaatgaaa atggcggtgc catactggaa gtaaatgcag cacccggatt
     8461 ccgcatgcat attgaaccgg cagaagggtt gc
//
LOCUS       DAORLS010000124         8414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4569_length_8414_cov_2.089724, whole genome shotgun sequence.
ACCESSION   DAORLS010000124 DAORLS010000000
VERSION     DAORLS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_8414_cov_2.089724"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>483)
                     /locus_tag="PK977_10365"
     CDS             complement(<1..>483)
                     /locus_tag="PK977_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18566.1"
                     /translation="NAITVFEKLLAVNPNNIEAIYWLGQTKLETDEIMGARLKDVRSI
                     YEKALQATNGAPLIKVGMGHIDLLTNKNEDARQHFESALTMTRTKKGDDPVISTAIGR
                     AITDTKNGDFAYAIRLLEDAASKDPKNTETLIQLGNAYRKARPGEGGGLAFQTYKKAI
                     E"
     assembly_gap    484..502
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(647..1024)
                     /locus_tag="PK977_10370"
     CDS             complement(647..1024)
                     /locus_tag="PK977_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRF18567.1"
                     /translation="MAARSSEGVIDYVAKTPGVIGFIGVSWIGNPEDSSHLSFLKKVK
                     VVSLESTDIPDAYVKAYQANIYIKRYPMVRDLVYILKENHKGLGNGFANFMSGEIGQM
                     IFRRAYLAPAQKNFGIRPIRLNE"
     gene            complement(1093..1584)
                     /locus_tag="PK977_10375"
     CDS             complement(1093..1584)
                     /locus_tag="PK977_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRF18568.1"
                     /translation="MSCRTIMKIPGLFILLVMILVSSVGCKSFKEQEDELPDNRRKGV
                     IYVSADESFKPIIDEHVKVYESNYPGTKINVEYKPEAECLKDMLIDSIRLVITTRTYH
                     QDEKDFIVDSLRKSPRSMVVARDAIAVIVHPDSHDTLFTMSEIREILTGKYKKKLIPV
                     LTA"
     gene            complement(1581..1823)
                     /locus_tag="PK977_10380"
     CDS             complement(1581..1823)
                     /locus_tag="PK977_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18569.1"
                     /translation="MFEDYEKRKRNQVSLMKSLMDYGMGLIILLLGVFFFFRNQFKLS
                     FNERFPPDDIDKILGVMCIIYGIWRIYRGYKKNYFK"
     gene            complement(1860..2687)
                     /locus_tag="PK977_10385"
     CDS             complement(1860..2687)
                     /locus_tag="PK977_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF18570.1"
                     /translation="MTNKEILKADLLDIIFENRNKDYGAYALRRGYNVRMLTALGAGL
                     SVILLFALINSWKKETSNAGHQVSKERNEVVIRSIQMPQEKPKEPEQPKQTSKPKPAP
                     KVAEIKFTNPKITEDNKVKEPLPPVEDLSGKLTSTQNVSGIPDDGKEKPVTKLNETVG
                     TGTAGPSQPVADFIIQERDPEFPGGAEALKKFLARYLSTPDELEAGEKKVVKVKFKVD
                     KDGSVNTFEIVTSGGDEFDGEVLRVVKRMPRWVPAIQNGINVPVSYVLPVTFIGMEE"
     gene            2804..>3155
                     /locus_tag="PK977_10390"
     CDS             2804..>3155
                     /locus_tag="PK977_10390"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRF18571.1"
                     /translation="MKILMVCLGNICRSPLAEGILQDKAFKAGLTWSIESAGTNNYHT
                     GEPPHHLSQKVAKINGIDISHQRARRFSASDFATYDKIYALAGDVLDDIKRIAKDKFD
                     PSKVDLLLNEIHPGK"
     assembly_gap    3156..3245
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3306..>3911
                     /locus_tag="PK977_10395"
     CDS             3306..>3911
                     /locus_tag="PK977_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRF18572.1"
                     /translation="MSKPTLPQGTRDFGPEVVRKRNYILNTIKSVFETYGFQPLETPA
                     MENIDTLMGKYGEEGDKLIFKILNNGLDNPEKEKQIREDFEKIIQGKNTKGITERALK
                     YDLTIPFARYVAMNHNQLTFPFKRYQVQPVWRADRPQKGRYREFYQCDADVVGSNSLL
                     NEVELALMYHTVFNNLQLSGSELRVNHRKILAALAAICGGAD"
     assembly_gap    3912..3930
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3931..>4176
                     /locus_tag="PK977_10400"
     CDS             <3931..>4176
                     /locus_tag="PK977_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRF18573.1"
                     /translation="ITIAIDKLDKIGLDKVKEELAQRGLTNEQVSTIEKYLSVTGSNK
                     EILKGLEALIGNTETGKMGIEELQFLLTQLSTHNVDLI"
     assembly_gap    4177..4340
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4341..>4659
                     /locus_tag="PK977_10405"
     CDS             <4341..>4659
                     /locus_tag="PK977_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF18574.1"
                     /translation="VDRIYDVMEELQLFPKDVHIGTQVLFFNLGEKESRYSFELMQQL
                     RDKGIRCELYHENSKFDKQFKYAEKKNIPYAIIIGEAEMMENKCKIKRLATGEQETIP
                     SGFL"
     assembly_gap    4660..4695
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4754..5350)
                     /locus_tag="PK977_10410"
     CDS             complement(4754..5350)
                     /locus_tag="PK977_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18575.1"
                     /translation="MKNKLIYTITGLMTVLLFSSCIKQLDKTFQGNTVVEIDPTPLNS
                     ANATAGYPVLTRIPAFGIPLRTSDSTLRRLNGTVRIRVNLVGPQSDQSQTVGYKIMAA
                     SPLTSIAFPATASGQTPSASAATLAVQNAVSGTHYTALSGTCTIPPQSSFGYIDVAII
                     NNGPTAGQARFLGIQLDSSGTLKPNPNYNKIGLVIDQR"
     gene            complement(5371..6882)
                     /locus_tag="PK977_10415"
     CDS             complement(5371..6882)
                     /locus_tag="PK977_10415"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF18576.1"
                     /translation="MKIKTFKILSLAVCSALLISSCKKQLDIPSRNSLDAGVALTTKN
                     GIEYAINSVYSILKRESHYGRDLFSVTDAMADIAFANGRSSRLLAENRNTALANVIIW
                     AGSYAAINEINLTLAAYPAISDATAAEKARWEGELKFLRAFYYFDLVRNYAYIPTYVV
                     SNQDKGGVVINLQGFNSAAAATGYFPSRSSISECYSQIWSDLYRSMELLSNANKGRNY
                     ASKHAALALGSRIALYEGNWARCDSFATAAITLSGGIGSLTNTSNYVTGWRAADHPEA
                     IFQVWYATLAENLGVNTSLAATHTTLSAPGAFAGVRQGQGDLVPNAFLLTQLGISGFP
                     TNLAQSPPPPALTYSNDIRNRLFEWGVNASGHYVECTKWMGKNGGPNWDNTVVLRWAE
                     LYLNRAEARFRLANEAGAWSDLNVIRSNRYVGFTVPPVPLTGQALLDEIWRQRMLEFA
                     FEGHRFYDLKRNGLSIVKTNPAVNMPATDYRLLPRIPTSEVDGNPNLVQNFGY"
     gene            complement(6901..>7413)
                     /locus_tag="PK977_10420"
     CDS             complement(6901..>7413)
                     /locus_tag="PK977_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18577.1"
                     /translation="RKIIGDIYPSKFGGWNNTLSYKGFSLDAFFQYEYGRVRLDGQYQ
                     QMMRMAGATVNTLSYGFITRWQNPGDIVPTPRAFNGLADYNSAGWGTGSRYIFRTDYI
                     RLKQVTLGYDIPANFAKKYRLEGARVYVQGINLWTYTEWPSYDPEFTGDNFGIIPQSK
                     NITVGVQVRF"
     assembly_gap    7414..7423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7424..>8414)
                     /locus_tag="PK977_10425"
     CDS             complement(<7424..>8414)
                     /locus_tag="PK977_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18578.1"
                     /translation="RLSDQSDWNTNLISTTTANFNKTFKDVHNVTALAGIEYRRDQNQ
                     WIQADAQGFPSYLLQYLSAASTPVAVSGQWTASATFSQFGKLGYSYNSRYIFNYTFRR
                     DGSSRFGANNKYGIFQSAQFAWNVKEENFLKKVNAISDFKLRYSFGQAGNDQIGNVLY
                     AQLYSATRLYGNASAIFPSQLGNPDLTWENREESNIGLDLGFFKNRITLTVDAYKRIN
                     KDLLLARPLYSTTGFTSIQQNLGKMENKGLELMLSVTPFDGKFKWTSTFNIAFQKNKV
                     LELYDGLQSLPSDASIRVGSPIGSFFTAEWAGVNPATGRGMWYDINGNLTYNPT"
BASE COUNT         2323 a         1745 c         1599 g         2409 t
ORIGIN      
        1 ctcgatagct tttttatagg tctgaaaggc caaacctcca ccttcaccgg gtcttgcttt
       61 gcgataagca ttcccaagtt gtattaatgt ttcagtattt tttgggtctt ttgatgccgc
      121 atcttccagc aaccttatag cataggcgaa atcaccattt tttgtatctg taatagcccg
      181 gccaattgct gtgctgataa caggatcatc gccctttttg gtacgggtca tggttagtgc
      241 agattcaaaa tgctgacggg catcttcatt tttattagtc agcaagtcta tatgacccat
      301 gcccactttg ataagtggtg ccccattagt tgcctgcagc gctttttcat aaatgcttct
      361 gacatccttt aacctggctc ccataatctc atctgtttcc agttttgtct gacccaacca
      421 gtaaatagcc tcaatattat tggggtttac agcaagcaat ttttcaaaaa ctgtgatggc
      481 attnnnnnnn nnnnnnnnnn nncggcatag aggttattta ttccctcctg cagtgtctgt
      541 gcctgcaccc caaccgatag cagtcctgat gcaagtagtg taattaaggt tttcttcatt
      601 ttacttcttc ttttaaaaag tttgtaaata taactaactc cttcttttac tcattcagcc
      661 ttattggccg gattccaaaa tttttttgtg ccggtgccaa ataggccctt ctgaatatca
      721 tctgtccgat ctctccgctc ataaaattcg caaatccatt tcctaagcct ttgtggtttt
      781 ctttcaatat ataaaccaaa tcccgtacca ttggataccg cttaatgtaa atgtttgcct
      841 ggtaagcttt aacatatgca tccggtatat ctgtactttc cagtgatacc acttttacct
      901 ttttcaaaaa actaagatga cttgaatcct ccgggtttcc tatccagctt acaccaatga
      961 aaccaatcac ccccggggtt ttcgccacat aatcaatcac accttcacta ctgcgtgccg
     1021 ccattgcttc cggtgtcaat gaatcacccc ttaacactga atcgatgaca aaacgtactg
     1081 tactggttgc ttttacgccg tcaaaacagg aattaacttt ttcttgtatt taccggtaag
     1141 tatttccctt atttcagaca tggtaaataa tgtatcgtgt gaatcggggt gaacaattac
     1201 agcaatggca tcccttgcca caaccatgct cctcggcgat ttccgcaatg agtcaactat
     1261 gaagtccttt tcatcctgat gataagtcct tgtcgtaata accagacgga tgctgtcaat
     1321 cagcatgtct ttcaggcatt cagcttccgg tttatattcc acattgattt ttgtgccggg
     1381 ataattggat tcatatacct taacatgctc atcgatgatg ggtttaaatg attcatcagc
     1441 gctgacatat attacgcctt ttctcctgtt atccggcagt tcatcttcct gctccttaaa
     1501 cgatttacaa ccaactgatg atacaagtat cataaccagg agaataaata aaccaggtat
     1561 cttcataatc gtacggcaac tcatttaaaa tagttttttt tatacccccg gtaaatcctc
     1621 cagataccat agattatgca cattacaccc aaaatcttat caatatcatc cggcggaaat
     1681 ctttcattaa aagaaagctt aaactggtta cggaagaaga aaaacacacc taacaaaagg
     1741 atgatcagtc ccatcccata atccatcagg gatttcatca acgaaacctg gtttcgtttt
     1801 ctcttttcat aatcttcaaa cattgccatt tctttacggg gcaggcaaat tattaaaatt
     1861 tattcttcca tccctataaa tgttacaggc agtacatagc ttaccggcac attaatgcca
     1921 ttctggatgg ccggcaccca acggggcatc ctttttacca ctctaagtac ttcgccatca
     1981 aactcatcac caccgctggt gacaatttca aaagtattta cactaccatc cttatctact
     2041 ttaaatttca ctttcaccac cttcttttct cctgcctcca gttcatccgg cgtagaaagg
     2101 taccgggcaa ggaatttttt taaagcttcc gcaccacccg gaaattcagg atctctttcc
     2161 tggataatga agtcagcaac aggctgggaa ggccctgcag tgccagtgcc gacagtctca
     2221 ttcaatttag ttacaggttt ttcctttcca tcatcgggaa ttccgcttac attttgtgtg
     2281 gaagttaatt ttccgctcag gtcttcaacc gggggcaatg gctcctttac tttattatcc
     2341 tctgttattt ttggattggt aaattttatc tctgccactt tgggtgctgg ttttggtttc
     2401 gaagtctgct tcggttgttc aggttctttt ggcttttcct gtggcatttg aatagatcgt
     2461 attactactt catttctttc ctttgacacc tggtgaccag cattgctggt ttcctttttc
     2521 caagaattga tcagggcaaa taataaaatg acagataaac cggcacctaa tgcggtaagc
     2581 atacgaacat tatatcccct tcgtaatgca taagcaccat aatccttatt cctgttctcg
     2641 aagatgatat cgagtaaatc ggcttttaaa atttctttgt ttgtcatagg tgtgtgtttg
     2701 agaaatagat gcagtgatca attaaaatcc ataacaagga ttgtcatcat ttcttcatac
     2761 aaattgactt ggtgcataca gcacgaatct tacctttgtc cttatgaaaa ttttaatggt
     2821 ttgcctcggt aatatttgcc gaagtccgct ggcagaagga atattgcagg ataaagcttt
     2881 taaggcaggt ttaacatgga gtatcgaaag tgccgggacc aataactatc atactggcga
     2941 accgcctcat cacctctcac aaaaagttgc aaagatcaac ggtatagata tcagtcatca
     3001 aagagcaagg agattttcag ccagtgattt tgcaacttat gacaagatat atgctttagc
     3061 cggcgatgta ctggatgata taaagcgaat tgcaaaggat aaatttgatc cttccaaagt
     3121 agatctgctg ttgaatgaaa ttcatccggg gaaaannnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnacaat aatcagcaaa tacagattaa ggatacctga agcaaatctt tgacctttca
     3301 atgatatgag caaaccaaca ctaccacagg gaacaagaga ttttggacca gaagttgtcc
     3361 gcaaaaggaa ttatattttg aatacaataa aatctgtatt tgaaacatat ggatttcagc
     3421 cattggaaac accggcgatg gaaaacattg atacgctgat gggcaaatat ggcgaagagg
     3481 gtgataagct catttttaaa atactgaata acggattgga taatcccgag aaagaaaagc
     3541 aaataaggga agatttcgaa aagataatac agggtaagaa tacaaaaggc attacagaaa
     3601 gagcattgaa gtatgacctt actattcctt ttgcccgtta tgtggccatg aaccataatc
     3661 agctgacttt cccttttaaa cgttaccagg tacaaccagt atggcgggcc gacaggcctc
     3721 agaagggacg ttaccgtgaa ttttatcaat gtgatgccga tgtggtggga agtaattccc
     3781 tactcaacga agtggaattg gctctcatgt atcatacggt ttttaataat ctgcagctca
     3841 gcgggtctga actaagagtc aaccatcgaa aaattttggc agcactagca gcaatttgtg
     3901 gcggagctga tnnnnnnnnn nnnnnnnnnn atcacaatag ccatcgacaa acttgataaa
     3961 attggattgg ataaagtaaa agaggaactg gcacaacgtg ggttaacaaa tgaacaggta
     4021 agcaccatag aaaaatatct ttctgtaacc ggcagtaata aagaaatact taaagggctg
     4081 gaagcattga tcggaaatac agaaaccggc aaaatgggaa ttgaagaact gcaattttta
     4141 ttgacacaac ttagcactca caatgttgat ttgatcnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn gtgttgaccg catttatgat gtaatggagg aactccaatt
     4381 gttcccgaaa gatgtacata tcggtacaca ggtattattt ttcaatcttg gagaaaaaga
     4441 aagtcgttat tcctttgagc tgatgcaaca attaagggac aaaggaatcc gctgtgaact
     4501 gtatcatgag aacagcaaat ttgacaagca gttcaaatat gcagaaaaga aaaatatacc
     4561 ttatgctatt attattggtg aagctgagat gatggagaac aaatgcaaga taaaaagatt
     4621 ggctaccggc gagcaggaaa ctattccttc aggatttctn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnngacct agtatgaaag aaaaagggct gctaatgcag ccctttttct
     4741 tttcagcaat tttctaacgc tgatctatta caagccctat tttattgtaa ttaggattgg
     4801 gctttagtgt tccggatgaa tccagttgta ttcccaggaa ccttgcctgt cctgcagtgg
     4861 ggccattatt aatgatggcc acatcaatat acccgaaaga actctgcgga ggaattgtac
     4921 aggtaccact cagcgcagta taatgtgtgc ctgaaacagc attctgcaca gctaatgttg
     4981 ccgctgaagc agaaggagtt tgccctgaag ctgttgcggg aaaggcaata ctggtaagcg
     5041 gagatgctgc cataatcttg taacctacag tttgtgactg atctgactgt ggtcctacaa
     5101 ggttcacccg aattcttaca gtaccattta acctgcgtaa ggtagagtca gacgttctta
     5161 aaggaatacc aaatgcaggt attcttgtta ataccgggta gccggcagtt gcattagcag
     5221 agtttaacgg agtgggatct atttccacaa ccgtatttcc ctggaatgtt ttatcaagct
     5281 gtttgatgca ggaactgaat aacaatacgg tcatcagtcc tgtaattgta tatataagct
     5341 tattcttcat tttcttttgt tttatgggta ttaatatcca aaattttgaa caaggtttgg
     5401 attaccatcc acttcgcttg tcggaatccg gggcaatagc ctgtaatctg tagcaggcat
     5461 atttacagca ggattagtct ttacaattga caaaccattt ctttttagat catagaaacg
     5521 atgtccttca aaagcaaatt ccagcattct ttgtctccat atctcatcca gtaatgcctg
     5581 gccggttaat ggaacaggtg gtacagtaaa gccaacgtac ctgttactac ggatcacatt
     5641 cagatcgctc caggcacctg cttcgtttgc cagtctgaac cttgcctctg ccctgttcag
     5701 gtataattcg gcccagcgga ggacgactgt gttatcccag ttcgggccgc catttttacc
     5761 catccacttt gtacactcca cgtagtggcc tgatgcattt actccccatt caaacagcct
     5821 gtttcggata tcattgctat aagtaagtgc cggaggtggc ggagattgtg ccaggttggt
     5881 tggaaaacct gaaataccca attgagttaa aaggaatgca ttgggtacta aatcaccttg
     5941 tccttgcctt acaccggcaa atgcacccgg tgcggataaa gttgtgtggg tcgctgccaa
     6001 agaagtattt acgcccaggt tttccgccag tgtagcgtac catacctgga aaattgcttc
     6061 cggatgatca gcagcccgcc agccggtaac atagttactt gtattggtca gagaaccaat
     6121 tcctccggac aaagtgattg ctgcagtagc aaatgaatca caacgggccc agttgccttc
     6181 atacaatgct attcgggaac ccaatgccaa agcagcatgc ttgctggcat agttacgtcc
     6241 cttgttcgca ttggacaaaa gctccattga cctgtagagg tctgaccata tctgggaata
     6301 acactcactt atggatgacc tcgaaggaaa ataacctgta gcagcagcag cagaattaaa
     6361 accttggaga ttgataacta cgccaccttt atcctggttg ctcactacat aagtagggat
     6421 ataggcataa ttccgaacca ggtcgaaata atagaatgca cggagaaact ttagctctcc
     6481 ttcccatctt gctttctctg cagctgtggc atcgctaata gcagggtaag ctgccaacgt
     6541 caggttaatt tcattaatag ccgcataaga acctgcccat attataacat tggccaacgc
     6601 cgtgttacgg ttttctgcca gcagtcggga tgaacggcca ttcgcaaaag caatatctgc
     6661 catcgcatcc gttacagaaa aaaggtcacg gccgtagtgg ctttctcgtt taagaatgga
     6721 ataaaccgaa ttgatagcat actcgattcc attttttgta gtaagcgcta caccagcatc
     6781 aagagagttc ctggaaggga tatcgagttg ctttttgcag gatgatatca gcaaagctga
     6841 acatactgca agcgataata tcttaaatgt tttgattttc atgatgttca tttttttatt
     6901 ttagaaacga acctgtacac ctacagttat gtttttggat tgagggataa taccaaaatt
     6961 gtcgccggta aactccgggt cataacttgg ccattctgta taagtccaca ggttaatacc
     7021 ctgaacatat acccttgcac cttccagcct gtattttttg gcaaaattgg caggtatatc
     7081 atatcccaat gttacttgct tcaaccttat ataatctgtt ctgaagatat agcggctacc
     7141 ggttccccag cctgcactat tgtaatcagc cagaccattg aaagcacggg gtgtgggaac
     7201 gatatcacca ggattttgcc agcgggtaat aaagccatat gaaagcgtat taacggttgc
     7261 acctgccata cgcatcattt gctgatactg accatctagt cttacacgac catattcata
     7321 ctggaaaaag gcatccagcg aaaatccttt atagcttaat gtattattcc atccgccaaa
     7381 cttgctcgga tagatatcac caataatctt acgnnnnnnn nnncagttgg attatatgta
     7441 agattaccat tgatatcata ccacatgccg cggccagtag ccgggtttac acctgcccat
     7501 tctgctgtaa agaaagatcc aattggcgaa cctacacgaa tggaagcatc agaaggtaat
     7561 gactgcaggc catcatatag ttcaagtact ttattttttt ggaaggcaat attaaatgta
     7621 ctggtccact tgaatttgcc atcaaaagga gtaacgctga gcattaattc cagtccttta
     7681 ttctccatct tacccaggtt ttgctgtatg gaagtaaaac cggtggttga gtacaatggc
     7741 ctggctaata ggagatcttt gtttattctt ttgtaagcat caactgttaa agtgatcctg
     7801 ttcttgaaga aaccaaggtc taatccgata ttactttctt ccctgttttc ccatgtcaga
     7861 tcggggttac ctaactgact ggggaaaata gctgaagcat taccatataa tcttgttgca
     7921 ctgtacaact gcgcataaag cacatttccg atctggtcat tacctgcttg gccaaatgaa
     7981 tatctcaatt tgaaatctga aattgcattc acctttttca ggaagttttc ttctttcaca
     8041 ttccatgcaa actgtgcaga ctggaaaata ccatacttgt tgttggcccc aaaccggctt
     8101 gagccgtcac gacggaatgt ataattgaaa atataacggc tattataaga ataccccagt
     8161 tttccaaatt gagagaaagt agcactagct gtccactgtc ctgatactgc cactggtgtg
     8221 gaagcagcac tcaggtattg taataggtag gatgggaaac cttgtgcatc agcctgtatc
     8281 cactggttct ggtcacgacg gtattcaata ccggccaatg cagttacatt atgaacatct
     8341 ttgaaagttt tattaaagtt ggctgttgtt gtgctaatga ggtttgtatt ccagtcactc
     8401 tgatctgaca aacg
//
LOCUS       DAORLS010000125         8411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4572_length_8411_cov_1.897319, whole genome shotgun sequence.
ACCESSION   DAORLS010000125 DAORLS010000000
VERSION     DAORLS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4572_length_8411_cov_1.897319"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK977_10430"
     CDS             complement(<1..615)
                     /locus_tag="PK977_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRF18579.1"
                     /translation="MNTEFPIKRIAIFGSTGSIGTQALEVIAANPEKFSADVLTAHHN
                     DELLIEQALKFNPNVVVIGDEKKYSKVKEALAATDVKVFAGEKALEEVAAMNCYDLML
                     AAIVGYAGLKPTLKAIENGKAIALANKETLVVAGDIIMKKAVENRVPIIPVDSEHSAI
                     FQCLVGETRNRIEKIILTASGGPFIGRKPNYLVNVKREHALQHPN"
     gene            complement(620..>1348)
                     /locus_tag="PK977_10435"
     CDS             complement(620..>1348)
                     /locus_tag="PK977_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF18580.1"
                     /translation="IGRKIDTIELFPASEGFFAFQDSQETEGLLLNTNSGIFFEFVPA
                     GEIFNEHPTRLSLKDVKVGENYALIINNNAGLWGYNIGDTVKFVSTNPYRLVVTGRTK
                     HFISAFGEHVIGEEVEYSMLKAAEEENIRVTEFTVAPFVSTDKGKSYHEWFVEFEKQP
                     ADMEAFSSKLDHYLREKNVYYDDLIAGNILKPLKITPVRKNGFIDYMKSIGKLGGQNK
                     VPRLSNDRKIADELIRFSESSILV"
     assembly_gap    1349..1370
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1371..2136)
                     /locus_tag="PK977_10440"
     CDS             complement(<1371..2136)
                     /locus_tag="PK977_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF18581.1"
                     /translation="MKIKSFLARPFASYIYKGIRKGMATAVADQENIFRQMLKVGRNT
                     DFGKEAGLEKVTDYEGFKQAVPIRDYEQFKPWIERIKEGKHNVLWKGKPIYFAKTSGT
                     TSGTKYIPITKDSIPNHINTARNALLCYMAETGNTKFADGKLIFLSGSPELERVGGIP
                     TGRLSGIVNHHVPKYLRTNQMPSYETNCIEDWENKLAKIVEETIHQNMTLISGIPPWM
                     QMYFDELIKRSGKKVGDLFPNFSVMVQGGVNFEPYKA"
     gene            2282..2581
                     /gene="rplU"
                     /locus_tag="PK977_10445"
     CDS             2282..2581
                     /gene="rplU"
                     /locus_tag="PK977_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRF18582.1"
                     /translation="MIAVVKVAGQQFKVEKDQTLYVPHVEGNTGDKLDLEVLLVDADG
                     KLSVGSSVNTKVQAEIVDQVKGDTVIAYKQKRRKGFHKKKGHRTAYTKIKVTNIA"
     gene            2624..2884
                     /gene="rpmA"
                     /locus_tag="PK977_10450"
     CDS             2624..2884
                     /gene="rpmA"
                     /locus_tag="PK977_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRF18583.1"
                     /translation="MAHKKGEGSVKNGRDSQSKRLGVKIFGGQPAASGNIIVRQRGTV
                     YHPGKNVGVGKDYSLFALADGVVEFRKGRNNRTYVSVTTAEA"
     gene            3165..4838
                     /locus_tag="PK977_10455"
     CDS             3165..4838
                     /locus_tag="PK977_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF18584.1"
                     /translation="MENAAIADNFSLLSKLMDIHGENSFKSKTYSIAAFNIEKLPVEL
                     KETPREKLFSIKGIGDSIGRKVIEMLDTGRLQVLDEIIANTPPGVIEMLNIKGIGPKK
                     IHTIWKEMGIESIGELLYACNENRLTLYKGFGEKTQQNVQESIEFYFQNQGHFLYAQL
                     NEIFPQINNYLKKIFTPGKVEVTGRYRRQELTIEELEFVILEPNEIVKPKFQTAQPPD
                     LLEENERSLLFKLKNGLKLRLYTGGSSMAHELFTTTGSGEFLQAFKSMFPELKYDELE
                     TDDDRSVFEKAGIPYIHSCLRETSTIIEKAQQNKLPQLIQTSDIRSIIHSHSNWSDGT
                     NSLEEMAQECIKRGYEYLVISDHSQTAAYANGLKEDRILAQHKQIDELNKRLTPFKVF
                     KSIESDILNDGSLDYPETILKSFDLVIASVHSNLKMTEEKALSRLIKAIENPYTTILG
                     HMTGRLLLSRNGYPIDHKKIIDACAVNRVVIEINAHPRRLDMDWRWISYAMEKNVLLS
                     INPDAHFTEGFDDVKFGVLAAQKGGLTKENNLSSFSLTEFESWLINRKK"
     gene            complement(4941..5195)
                     /locus_tag="PK977_10460"
     CDS             complement(4941..5195)
                     /locus_tag="PK977_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRF18585.1"
                     /translation="MKKGIHPESYRLVVFKDMSNGHSFLSRSTAASKETIKWEDGNEY
                     PLVKLEISNTSHPFFTGKNVLVDTAGRIDKFKKKYAKKAE"
     assembly_gap    5486..5523
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5649..6134)
                     /locus_tag="PK977_10465"
     CDS             complement(5649..6134)
                     /locus_tag="PK977_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRF18586.1"
                     /translation="MPSFDIVSKVDAQALDNAVNVTTKEITNRFDFKGSHVVIDLDKK
                     EFNIKLETEDDMKMRQLIDVLISRAHKQGIAPEAFDTSKQGAQSGKVWRKEVKVRNVQ
                     EDAKKIVKLIKDSGLKVQASINDDLVRVTGKKIDDLQSVIQLCKTSNMGIPLQYVNMR
                     D"
     gene            complement(6235..>8411)
                     /locus_tag="PK977_10470"
     CDS             complement(6235..>8411)
                     /locus_tag="PK977_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF18587.1"
                     /translation="NELKVQPIILTEAIKDLAGVTITAKKPFIETKIDKTVVNVDASP
                     TSAGATALEVLEKSPGIMVNNDGAISLRGKAGVIVMMDGKPTFLSPTDLANLLKNMPA
                     SAIDQIEIMTNPSAKYDAAGNSGVIKIKTKKGRAAGFNGSIMAGITTSFFETNNTLYV
                     IPKSQNSINFNYRKNKINFFGNYNPNFFRGRSTLVISRRKIDENKTVLGYNDVLTQFK
                     FGNNNHTLKLGLDYSPNKKNTFGIVVSGFMFSGHPTPKTVTSTHDADYKLLSQMVSQT
                     DNRLRFRNFTSNFNYRHQFDSTGRELTADFDFIAYDNTSRMTLTTDFYGGSGQPIGDA
                     LLLKGHLPSSINIYSLKSDYVHPFKKGGRIEAGIKSSFVKNNNLVNYERWDNSKWIAD
                     GRSNHFIYDENINAIYLNANKQIKKWSFQGGLRLENTSAKGYQVTNDSTFKRNFTNLF
                     PSAFISYAIDKSNQVTVSYSRRITRPNYQDLNPFIYFLDSLSYRKGNPFLLPQFTHNI
                     ELSHSFKGKLITTLNYNNTKNVISQLVKPDGDLLYLTSDNVAKFRNIGIAITAPIPVT
                     KWWNSNFFVNVFNNHYEGVYENKPLDLSYTSFMVNMTQTFTLKQGFTLEMSGFYRARG
                     VDQLNINEPMYVLSFGGQKQVLKGKGTVRLNLRDPFWMQKYKGKTQYDIVDSRVQSRW
                     DNRQVTATFTYRFGKNGQQNPPPRRRNSASQDEQNRVGQGGQ"
BASE COUNT         2487 a         1738 c         1609 g         2517 t
ORIGIN      
        1 gttggggtgc tgcaacgcat gctctcgttt tacattcacc aggtagttgg gtttgcgacc
       61 gatgaacggg ccgccggatg ctgtcaatat aattttttca atcctgtttc ttgtttctcc
      121 aaccaggcat tgaaaaatag ccgaatgctc tgaatctact ggtatgatag gtacccggtt
      181 ttctacagcc tttttcatga tgatatcgcc ggctaccacc agggtctctt tattggctaa
      241 tgcaatagct ttaccatttt caatagcctt aagggtgggt ttcaggccgg cataacccac
      301 aatggcagcc agcattaggt catagcagtt cattgctgct acttcttcca gggctttttc
      361 tccggcaaag acttttacgt cagtagccgc caacgcttct tttacttttg aatacttctt
      421 ctcatccccg atcaccacca cattaggatt aaatttcagg gcttgctcaa tcagtaattc
      481 atcattatga tgtgcagtaa gtacatcagc ggaaaatttt tcaggattag cagctatcac
      541 ttccagtgcc tgtgttccta tggaaccggt ggacccgaat atagcaatgc gtttgatggg
      601 gaattcagta ttcattgatt tatactaata tgctgctttc actgaatctg atcagctcat
      661 cagcaatctt tctgtcgtta ctcagccttg gcactttatt ttgtccgcct aatttaccaa
      721 ttgatttcat gtaatcaata aatccgtttt tgcggaccgg agttattttc aaaggcttaa
      781 gaatattacc agcaataaga tcgtcataat agacattctt ttcccgaaga taatgatcaa
      841 gtttgctgga aaaggcttcc atatcggcag gctgtttttc aaactctaca aaccattcgt
      901 gatatgattt ccctttgtca gtacttacaa acggggcaac agtaaattca gttacacgga
      961 tattttcttc ctcggctgcc tttagcatac tgtattctac ttcttctcca attacatgct
     1021 cgccaaaagc agatataaaa tgcttggttc tgcctgtcac caccagcctg tacggatttg
     1081 ttgaaacaaa tttcaccgta tccccgatat tatagcccca caaaccggca ttattattta
     1141 taatcaatgc ataattctct cccaccttaa catcttttaa cgacagccgg gtcggatgct
     1201 cattaaatat ctcaccggca ggcacaaatt caaaaaagat gccgctgttg gtatttaata
     1261 agagaccttc tgtctcctgg gaatcctgga aagcaaaaaa accctcactg gcaggaaaga
     1321 gctcaatcgt atcaatcttt ctgcctatnn nnnnnnnnnn nnnnnnnnnn tggctttata
     1381 aggttcaaaa tttacaccac cctgaaccat cacactaaaa ttgggaaaca agtctccgac
     1441 cttcttaccg cttcttttta taagttcatc aaaatacatt tgcatccatg gtggaatgcc
     1501 gctgataagt gtcatatttt gatggatagt ctcttctacg attttagcaa gcttgttttc
     1561 ccagtcttct atgcaattgg tctcataaga aggcatttgg ttggtacgca gatatttcgg
     1621 gacatgatgg ttaactattc cgcttaaccg gccagtggga atgcctccca ccctttccag
     1681 ttcaggagac ccggataaaa agatcagttt cccgtcagca aactttgtgt ttcccgtttc
     1741 cgccatgtaa cataataagg cattacgagc cgtattgata tggttgggga tagaatcttt
     1801 tgtgatagga atatatttgg ttccactggt ggtaccggat gttttggcga aataaatggg
     1861 cttccccttc caaagcacat tgtgcttacc ttctttgatt cgttcaatcc agggcttaaa
     1921 ctgttcatag tcacggatag gcactgcctg cttaaacccc tcgtaatcag ttactttttc
     1981 aagtcctgcc tcctttccaa aatcagtatt acggcccact tttagcattt gccggaaaat
     2041 attctcctgg tcagctacag ctgtagccat gcctttacga atacctttat atatataaga
     2101 agcaaacggc ctggctaaaa aagacttgat tttcatacac tatgcgctgt gaacgttcaa
     2161 aggtcagcaa atttaacggt aggcaggtca aaaaacgggc atttaattgt tcataaatct
     2221 ttgatttccc gcttgcggct tcaaattttc cccttacctt tgccgtccta atttttatag
     2281 tatgatagcc gtagtaaaag tagccggaca gcagttcaaa gtagaaaaag accagacttt
     2341 gtatgtacct catgtagaag gtaataccgg tgataaactg gaccttgaag tattgctggt
     2401 tgatgccgat ggaaaattat ccgttggatc ttccgtaaat acaaaagttc aggcagagat
     2461 cgttgaccag gtaaaaggtg ataccgtaat tgcatacaag caaaaaagaa gaaaaggttt
     2521 tcacaaaaag aaaggtcatc gtactgctta taccaaaatc aaagtaacaa atattgcata
     2581 agcaataagc taactaagta ttcaataaaa aaatcttcga gatatggcac ataagaaagg
     2641 tgaaggtagt gtaaagaacg gccgtgattc gcaaagcaaa cgccttggag taaaaatctt
     2701 cggtggtcag ccagcagcat ccggtaacat tatcgttcgt cagcgtggta ccgtttacca
     2761 ccccggtaaa aatgttggtg ttggtaaaga ctattcattg tttgcattag cagatggtgt
     2821 tgttgaattt cgcaaaggaa gaaacaacag aacgtatgtt tctgtaacaa ctgcagaagc
     2881 ataatcccaa cagccgatca gaattttgtg gaaaaaaaat ttgggtagaa agaaataatt
     2941 gttattttta ctatcgttaa cccattttta ctaacattaa attctaacaa aatggctact
     3001 aagaaaaaag ctgctaaaaa agctgctcct aagaagaaag ctgctaagaa agctgctaag
     3061 aaaaagaaat aagtttgatt gtttcttaaa agaaatagaa agtcctccga aattcggggg
     3121 acttttttta ttcccctgcc ttcaataaat agtaatttgc gtcaatggaa aatgccgcca
     3181 tagctgataa tttctctctc ctttccaaac tgatggatat acatggcgaa aactccttca
     3241 aatcgaaaac ctatagtata gctgcattca atatcgaaaa actgccggta gagctaaaag
     3301 aaacaccgag agaaaaactt tttagtatca aaggaatcgg tgatagtatt ggccgcaaag
     3361 tgatagagat gctcgatacc ggccggctgc aggtactgga tgagatcatt gccaacaccc
     3421 ccccgggtgt aatagagatg ctgaatataa aaggaattgg cccaaagaag atccatacta
     3481 tctggaaaga aatgggaatt gagtcaatcg gagaattgct ttatgcctgc aatgagaacc
     3541 ggcttaccct ttacaaaggg ttcggggaaa agacacaaca aaacgtacag gagtccattg
     3601 aattctattt tcagaaccag ggacattttt tgtatgccca gttgaatgaa atatttccgc
     3661 aaataaataa ctacctgaaa aaaatattta ctcccggtaa agtagaagta acaggtcgct
     3721 accgcagaca agaacttact attgaagaat tagaatttgt tattctggaa cccaatgaaa
     3781 tagttaaacc aaaatttcaa actgcacaac cgcctgattt attggaagaa aatgaaagaa
     3841 gccttctgtt taaattgaag aacggactta agcttcgttt atatacaggt ggcagttcta
     3901 tggctcatga actattcacc acaaccggaa gcggggaatt tctccaggca ttcaagagta
     3961 tgtttcctga acttaagtat gatgagttgg aaactgatga tgacagatcg gtatttgaaa
     4021 aagctggcat cccatacatt cattcctgtc ttagagaaac ttcaacaatc attgaaaaag
     4081 cccaacaaaa caagcttcca caattaattc aaaccagcga tatccgatcc attatacata
     4141 gccatagcaa ctggagtgac ggaaccaatt cattggagga gatggcgcag gaatgtataa
     4201 aacggggata cgaatacctg gtaatttcag atcattccca aacagctgcc tatgcaaacg
     4261 gattgaaaga agacagaatt ctggcacagc acaaacaaat tgatgaactt aataaacgat
     4321 taacaccttt caaagtcttt aaaagtattg aaagtgacat cctgaacgat ggaagcctcg
     4381 actatcctga aactatactt aaaagctttg acttggttat tgcttctgtt catagtaatc
     4441 tgaagatgac cgaagaaaag gcattgagca ggttaataaa agctattgaa aacccttata
     4501 caaccatcct gggtcacatg accggaaggt tattgctaag caggaacggc taccccattg
     4561 atcataaaaa gataatagac gcctgtgctg ttaaccgggt ggtgatagaa ataaatgccc
     4621 atccccgccg cctggatatg gattggagat ggatttcata tgcaatggaa aaaaatgttt
     4681 tattatccat aaacccggat gcgcatttta ctgaaggttt tgatgatgtg aagtttggtg
     4741 tactggcagc acagaaagga ggccttacca aagagaataa cctgagcagt ttttcgctga
     4801 cagaatttga atcatggctg ataaaccgca aaaagtaagg ttttaaaaaa aatgaccccc
     4861 cgaagaatcg ggaggtctta aagtatagcc atgaaaaaca aaactccctg ctttcgcaaa
     4921 gagctctgca caagttaata ttattctgcc ttttttgcgt acttcttctt gaatttgtcg
     4981 atacgtcctg cagtgtcaac caatacattt ttacctgtaa agaaaggatg tgaggtattt
     5041 gaaatctcca gctttaccag gggatattca ttgccatctt cccactttat ggtttcttta
     5101 gaagcagcgg ttgatctgct caggaaactg tgaccgttac tcatgtcttt gaaaactact
     5161 aagcggtagc tttccggatg gattcctttt ttcataactc tatgattttt aaaggctgca
     5221 cggattttgc cgtacagatg aaaagataaa atttgggggg caaagatagg ccggataggt
     5281 cttttgacca aatgtgattt aggggcaaaa tggccaaaaa gatgaaagcg ccaagtgagc
     5341 ttatgtcaga ctttcgcctt ccatttactt acccgacgct ttctggagaa acatatgcct
     5401 tttgaggggc ttctgatatc tccttcggat ccaactttca ccttttgagg ggtttccatt
     5461 ttctccggca cttcctttta ttgctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnntcaaata tactttctgc aattcccgtt ttccaaattt ctggccatca tgatttacac
     5581 tcgttatgca ccctgttatc caccaaaaaa tacccgattc cggcacttac aaacagaata
     5641 actgcaaatc agtcccgcat attcacatat tgcaagggta tgcccatgtt tgacgttttg
     5701 caaagctgaa tgaccgactg taagtcatca atttttttcc cggtcactct taccaggtca
     5761 tcgttaatgg aggcctgtac cttcaaacct gaatccttta tcagctttac gatctttttc
     5821 gcatcctcct gtacgtttct cacttttact tccttcctcc aaactttgcc gctttgagct
     5881 ccctgttttg atgtatcaaa agcttcggga gcaatccctt gtttatgggc acggctgatc
     5941 aatacatcaa tcaactgccg catcttcata tcatcttcag tttccagttt gatgttgaat
     6001 tcttttttat caaggtcaat taccacatgt gatcctttaa aatcaaaacg attggtaatc
     6061 tccttagtgg ttacattcac agcattatcc aatgcctgcg catctacttt actgacaata
     6121 tcaaaagaag gcatagcagt tagttttagt tgagtgacaa atatagaaaa gtccctctta
     6181 gaaaagagag actttcatta tcacatactg agaaaactaa cgactaatta cagcttattg
     6241 gccaccttgt cccaccctgt tctgttcatc ctgcgaagca ctgttgcggc gacgtggcgg
     6301 cggattttgc tgaccgttct tgccaaaacg ataagtgaaa gtggcagtaa cctgcctgtt
     6361 gtcccagcgg ctctgaaccc tggaatcaac tatatcatac tgtgttttac ccttatactt
     6421 ttgcatccag aacggatccc tgaggttaag acgaactgtg ccttttcctt tcaatacctg
     6481 cttttggcca ccaaaactca acacatacat tggctcattt atatttaact ggtctacccc
     6541 cctggcccga taaaatccag acatctccag cgtaaagccc tgcttaagcg taaatgtctg
     6601 tgtcatatta accatgaacg aggtgtatga caaatcaagc ggtttgtttt cgtacactcc
     6661 ttcgtaatga ttattaaata cattaacgaa gaagttggaa ttccaccatt tggttacagg
     6721 gataggtgct gtaatcgcaa taccaatatt ccggaattta gccacattat cagatgtgag
     6781 gtacaacaag tctccatcag gcttcacaag ttgcgagata acatttttag tgttattata
     6841 gtttaaggtg gtaatgagtt ttcctttaaa tgaatggctc aattcaatat tgtgagtaaa
     6901 ctgtggcaac aggaatggat ttcctttcct gtaagataat gaatcaagga agtagataaa
     6961 aggattcaga tcctggtaat tgggccttgt aattcttctg ctatacgaaa ccgtaacttg
     7021 attactctta tcaattgcat aactaataaa ggcacttggg aaaaggttag tgaagtttct
     7081 tttaaaagta gaatcattcg ttacctggta acccttggca gaagtatttt cgagcctcaa
     7141 gcctccctgg aaagaccact tcttgatctg tttattcgca ttcaggtaaa tggcgttgat
     7201 attctcatca tagatgaaat gattggaacg gccatcggca atccatttac tattgtccca
     7261 tctttcataa ttaacgagat tgttattttt tacaaagctg cttttaatcc cggcttcaat
     7321 tcgtcctccc tttttaaacg gatgaacata atcagattta agggagtaaa tattaataga
     7381 ggatggcaaa tgccctttca gcaataatgc atcaccgatt ggctggcctg agccaccata
     7441 aaaatcggtt gtcaaagtca tccttgatgt attatcatag gcaatgaagt cgaagtcggc
     7501 agtaagttct ctgcctgtag aatcaaactg gtgccggtaa ttaaaatttg aagtgaaatt
     7561 gcggaacctg agacggttat ccgtttgaga taccatttgg gatagcagtt tatagtctgc
     7621 atcatgtgta ctggtaactg ttttaggtgt cggatggcca gaaaacataa agccgctaac
     7681 tacaatacca aaagtatttt ttttattcgg tgaataatca agacccagct tcagggtatg
     7741 attattattg ccaaacttaa attgagtcag tacatcatta taacctaata cagttttatt
     7801 ctcatcaatt ttccttcggg atatcaccag tgtacttctg cctcgaaaaa aattagggtt
     7861 ataattacca aaaaaattga tcttgttctt gcggtaatta aaattgatac tgttttgtga
     7921 tttaggtatc acatacagcg tattatttgt ttcaaaaaag ctggtcgtta tccctgccat
     7981 gatagaacca ttgaaccctg ctgcccttcc ctttttcgtt ttaatcttaa taactcctga
     8041 attgccggca gcatcatatt tagcggaagg attcgtcata atctcaatct ggtcaatggc
     8101 agatgcaggc atatttttta acaggttggc tagatctgtt ggtgaaagaa aggttggttt
     8161 tccgtccatc ataacgatta cacctgcttt acctctcagg cttatagccc cgtcattgtt
     8221 gaccattata ccaggagatt tctccagtac ttctaaagcg gtggccccgg cacttgtggg
     8281 cgatgcatca acattcacta cagttttatc aatctttgtt tcaataaatg gttttttagc
     8341 tgtaatagta actcccgcca gatctttgat ggcttctgtc agtattatgg gctgaacttt
     8401 taattcattg t
//
LOCUS       DAORLS010000126         8275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4667_length_8275_cov_1.712044, whole genome shotgun sequence.
ACCESSION   DAORLS010000126 DAORLS010000000
VERSION     DAORLS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4667_length_8275_cov_1.712044"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK977_10475"
     CDS             complement(<1..278)
                     /locus_tag="PK977_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18588.1"
                     /translation="MIRILIADDHAIVRRGMKQILLEQYPSADVAEAGDAETLVAKVV
                     EGGWDIVITDMNMPGRSGLDALHQIKQIDPALPVLIMSMYPEDQYALRV"
     gene            complement(304..>533)
                     /locus_tag="PK977_10480"
     CDS             complement(304..>533)
                     /locus_tag="PK977_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF18589.1"
                     /translation="ANATKVIVKAEVKDKVLRLSIADNGTGFDTSKVKSKKTLGILGM
                     QERSSMMGGDYSITTTPGKGTTIVVSVPINL"
     assembly_gap    534..552
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<553..>1365)
                     /locus_tag="PK977_10485"
     CDS             complement(<553..>1365)
                     /locus_tag="PK977_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF18590.1"
                     /translation="DKKQTEEELIRNERKYRTLVEQAIDAIALYDANGKLLDVNTGAI
                     NLLGYTREELQHMSLKDVLTDDEIKVRPVQYDVLKTGKSTVKQRSMRKKDGTIVQTEV
                     RSQQLPDGRFLSVIRDLTDRIKAEEELKESYKAIRTLTDHLQKVREEERAHIAREIHD
                     ELGQQLTVLKMDIAWLNKKIDASDESPLKIKMKELLTMLDGTVRTVRRISSELRPSLL
                     DDMGLFAAMEWHLEDFSKRTGLETSLVAGKEPEKLPDNIKTGLYRIFQESLTN"
     assembly_gap    1366..1519
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1520..5112)
                     /locus_tag="PK977_10490"
     CDS             complement(<1520..5112)
                     /locus_tag="PK977_10490"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF18591.1"
                     /translation="MRRIYYTRNITLGLIISFVVITFFGWLGLVNLKMAKKETAIVDE
                     SLQSLRILEVILDDMQDIETATRGFVISGKEEFLAPYFVAIKRIANDTLDIKSLSALY
                     PERQSMYDTLQRLISQKLNLAELSIYHMKNQNKEAANRHVQDGEGRIVMDKIRQIIYS
                     FEDKDIKKLHESNDSRQKAANNLVLTILVIGLVFILMFVMLYRRVRIEMKKREEDERE
                     ILYLANLVQRTGDAIISLDMEGVILTWNPAAEELFGYQAKEAVGANFRDFTSTDFLQS
                     IAEEVKSELRLKGTSEAEISFSAKDGKEIKTLISVKHLYDKEDKPAGYVCVIRDVTQR
                     KLASDLLLKFNKELADKIKEKTEEIKKEEEKLKQVLDSAAGEFYVINKDYEIILISKP
                     SEFNLARAWGKPVKAGNKILDCIPPEKLDYIKSNFDKVFQGNEVEYESQIIVGGEPKW
                     IQVNYLPVKGSKNEITGSCVITRDITERKLADNQQRESLERFSLIGKATSDSVWEWNF
                     QTGKMWANENHQLMYGLTLNDPVPSTDEWLARIHPDERERVKLSQAEALESDSNVFIT
                     EYRFRFADNKYRYIYDRCYIVRNEQGKPVKMMGSMMDITDRIRAEEAIRVNEETQRLI
                     MNSALDAIVCIDTKSVITIWTPQAEKIFGWKAEEVIGLSLTDTIIPLRHRSSHKNAVN
                     NYLKTGQQKVLGRLIEISATNKSGNEFPVELSIVHIKQGSNEFFCAFIRDISQRKQAE
                     LEIRSSNERFELIAKTTNDAIWEWNLETGHLWGSDAHQKLYGLTTKDPVPDQSEWVKR
                     IHPEERETVLNDLRKAIADPGTNAWITEYRFLSGDQYLAIYDRSYIVRDGSGKAIRIM
                     GAMMDITERKQAEEELIKSRTRFRNLVENISGVYWVNDITRRQTLYISPSYELIWGRS
                     CEDLYKNPADFINSVHPDDKQYLFEEYEAIAQKRKLSIVYRIIRPDKTIRWIAAKVNV
                     VTDENGNDIEYGYGDDITEQKRAEEEMLKSTERFNIVSKATSDIVWDWFMAENTFWWN
                     DNYYSTIGVPRSPGIATIDEWFQRLHPEDYERVRKKFLGAIGGDQHFWSDEYRYRKND
                     GSYLNMYDRGYIIRNSSGIAIRMIGSMIDMTQMLKVQKEVEESENRLRTIFNSEPECI
                     KLLDINGQLLDMNPAGLSMIEADSLEQVKNKSVLGIINEDYRIPFASLCQDV"
     gene            complement(5164..>5392)
                     /locus_tag="PK977_10495"
     CDS             complement(5164..>5392)
                     /locus_tag="PK977_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRF18592.1"
                     /translation="QQELEDENGKPDLNALSRRELDIVKLIKEGLSSREIAVRLDISL
                     KTVEVHRYNILKKLNLKNTAALVNFINAQGL"
     assembly_gap    5393..5418
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5419..5806)
                     /locus_tag="PK977_10500"
     CDS             complement(<5419..5806)
                     /locus_tag="PK977_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18593.1"
                     /translation="MDKITILLVDDHKLIRDSWSFILNSDDRFRVVGETSDAEELVSL
                     AKEKKPRIVLMDINMSPVNGFEATKLVRQYSPGSKVIGISMHSMPAYARKMMQIGAMG
                     YVTKNSSKDELMNAIVEVNGGKKYICE"
     gene            complement(6046..6524)
                     /gene="ssrA"
                     /locus_tag="PK977_10505"
     tmRNA           complement(6046..6524)
                     /gene="ssrA"
                     /locus_tag="PK977_10505"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     assembly_gap    6314..6414
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6495..>7599)
                     /locus_tag="PK977_10510"
     CDS             complement(6495..>7599)
                     /locus_tag="PK977_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRF18594.1"
                     /translation="KEAMKQPAQPWEFRSKPAWQRLIVMLGGVIMNVLTAFVIYAMIL
                     MVWGEKKTPMSSLKYGIWTTDSLMNEIGLQNGDKVITVNGDTVRNYEDLNAKILLGKT
                     ISLERNGVKKDIELPVNLIEKLIESKRKRGVLLSPRIPSYVGKYDKKDTSFGKKAGLQ
                     EMDRIVAIDSTPIQFYDEIAAVLQQHKNGKVSLSVERNGQIIRLDSRVTEEGKIGIPY
                     LEDAQYAALGVYEINKRKYAFFEAFPAGVRKAGEKLQFYIDQFKKILNPKTGAYKGVG
                     GFKAMGSIFPTSWSWEAFWNITAFLSIVLAFMNLLPIPALDGGHVVFTLYEMITRRKP
                     NEKFLEYAQLVGMVLLLGLMLYANGNDWFGWGK"
     assembly_gap    7600..7679
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7680..7878)
                     /locus_tag="PK977_10515"
     CDS             complement(<7680..7878)
                     /locus_tag="PK977_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRF18595.1"
                     /translation="MSLLAINWSNVGLQAGQLLLALSLLVILHEFGHYITARWFKCRV
                     EKFFLFFDPWFALVKKKVGDTV"
     gene            complement(7934..>8275)
                     /locus_tag="PK977_10520"
     CDS             complement(7934..>8275)
                     /locus_tag="PK977_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRF18596.1"
                     /translation="ALAFPKRIPNNFPRYDFKKVNTLTFEEPDVKTFRNLALAQDALF
                     KGGNLPCIMNAANEIAVFAFLRNRINFLDMTNVIEKTMEQVPFIEHPTLEEYFDSDGE
                     ARNFAADIIKL"
BASE COUNT         2160 a         1835 c         1307 g         2593 t
ORIGIN      
        1 acacgaaggg catactggtc ctcaggatac atgctcatga tcagaacagg aagtgcggga
       61 tctatttgct taatctggtg taaggcatcc aatccactcc ggcccggcat attcatatcg
      121 gttatcacaa tatcccatcc gccttctact acttttgcga ccagggtctc cgcatcgcct
      181 gcctctgcca cgtcggctga gggatattgc tccagcaata tctgcttcat tcctcttcta
      241 acaatagcat ggtcatcggc aattaatatc ctgatcatac attacttatt cgttgacgct
      301 aaattacaag ttgattggaa cactcacaac tattgtagtt ccttttccgg gagtagtagt
      361 gatggaataa tctcctccca tcatggaaga acgttcctgc ataccaagaa ttcccagtgt
      421 cttctttgat ttcactttag aggtatcaaa tccggtccca ttatcagcaa tacttaacct
      481 aagcactttg tccttgactt ctgcttttac aatgacctta gtggcatttg catnnnnnnn
      541 nnnnnnnnnn nnattggtta atgattcctg gaaaatccgg tataaaccgg tcttaatatt
      601 atcaggtaac ttttctggtt ctttgcctgc taccaaagag gtttccaatc ctgttctttt
      661 gctaaaatcc tccaaatgcc attccattgc agcaaataat cccatatcat ccagtaaact
      721 tggtctcaat tctgacgaaa ttctccttac agtccttact gttccatcca gcatagtcaa
      781 aagttctttc attttaattt taagcggact ttcgtcggaa gcatctatct tcttatttaa
      841 ccaggctata tccattttga gtacagtcag ttgctgacct aattcatcat gtatttccct
      901 ggcgatatgg gccctttcct cttccctgac tttttgcaag tggtccgtca aggtacgaat
      961 ggctttatat gactccttca actcctcctc tgctttgata cgatcagtta aatctctgat
     1021 tacagacaga aagcggccat ctggtaactg ctgagaacgt acttcggttt gtacaatagt
     1081 gccatccttt ttgcgcatag atctttgctt aacagtagac ttaccagtct tcagaacatc
     1141 atattgaacc gggcgaactt taatttcatc atctgtaaga acatctttta aggacatatg
     1201 ctgcaattcc tctcttgtat aacccaatag gtttatggcc cctgtgttaa catccaataa
     1261 ttttccattt gcatcatata aggcaatagc atctattgcc tgttctacta gtgtccggta
     1321 tttcctttcg tttcttatta actcttcttc tgtttgtttc ttatcnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnna catcctggca aaggctggca aagggaatac ggtaatcctc
     1561 atttataatc cccagaacag atttattttt tacctgttct aaagaatcag cttcaatcat
     1621 tgataatccg gcggggttca tatcaagcaa ttggccatta atatcaagca gcttaataca
     1681 ctcaggctca ctgttgaata ttgtcctcaa tctgttttca ctttcctcta cttctttttg
     1741 cacttttaac atctgtgtca tatctatcat ggaaccgatc attcttatag ctatacctga
     1801 actatttctt attatatagc ccctgtcgta catgttgagg tagcttccgt catttttacg
     1861 atacctgtat tcatctgacc aaaaatgctg gtctcctccg atggcaccaa gaaatttttt
     1921 cctgactcgt tcgtaatctt ccggatgaag ccgctgaaac cattcatcaa tagtagcaat
     1981 gccgggagac ctgggcacac ctattgtaga ataataatta tcattccacc aaaaggtgtt
     2041 ctcagccatg aaccagtccc atactatatc gcttgtggcc tttgaaacga tgttaaatct
     2101 ctctgtagat ttcaacattt cttcttcagc ccttttctgc tcagtgatat catcaccata
     2161 accatactca atatcattac cattttcatc agttactaca ttcacttttg cagcaatcca
     2221 gcgtatagtt ttgtctggtc tgataatcct gtatacaata ctcagctttc gcttttgcgc
     2281 aatagcttca tattcctcaa acagatattg tttatcatcc ggatgaacag aattaatgaa
     2341 atcggcagga tttttatata aatcctcaca tgatcttccc caaatcaatt cgtaagatgg
     2401 actgatatat aaagtctggc ggcgagtaat atcatttacc cagtatacac cggatatatt
     2461 ctcaaccagg ttcctgaacc gggttcttga ctttattagt tcttcttcag cttgcttacg
     2521 ttctgtaata tccatcatag ctcccataat ccttatggcc ttgccagaac catctctgac
     2581 aatgtagctt ctgtcataaa ttgccaggta ctggtcaccg gataaaaacc tgtattcggt
     2641 aatccaggca ttggtaccag gatccgcaat tgcttttctc aggtcattta gtacagtttc
     2701 cctttcttct ggatgtattc ttttaaccca ttctgattga tccggaacag gatccttcgt
     2761 tgtaagtccg tataatttct gatgagcatc acttccccac aaatgtcccg tttcaagatt
     2821 ccattcccaa atggcatcat tggtagtttt ggcgatcagc tcaaatcgct catttgaact
     2881 tcttatttcc agctctgctt gtttcctttg actgatatcc ctgatgaaag cacagaaaaa
     2941 ttcatttgat ccctgcttaa tatgaacgat acttaattct accggaaact cattaccact
     3001 tttgttggtg gcagatattt ctataagcct gcccagcact ttttgctgac ctgttttaag
     3061 ataattatta accgcatttt tgtgtgagga acgatgacga agaggtataa tcgtatctgt
     3121 aagtgacaag ccaataactt cctccgcttt ccagccaaat attttctccg cttgtggtgt
     3181 ccagatagta ataacacttt ttgtatcaat acacacaatg gcatccagag cagaattcat
     3241 tatcagtcgc tgggtttcct catttactct tatagcctcc tctgcccgaa ttctatcggt
     3301 gatatccatc atacttccca tcattttgac gggcttaccc tgttcgttac ggactatata
     3361 acaacggtca taaatgtatc tgtacttatt atcagcaaag cgaaacctat attccgtaat
     3421 gaatacatta ctatctgatt ctaacgcctc agcctggctt agcttcaccc tttccctttc
     3481 atcagggtgt attcttgcaa gccattcatc agtggaagga acagggtcgt tcagggtcaa
     3541 cccatacatt aactggtggt tctcatttgc ccacatctta cctgtctgaa aattccattc
     3601 ccaaactgaa tcgctggtag ctttaccaat caggctaaat ctttcaagtg attctctttg
     3661 ctggttatca gctaattttc tttctgtaat atctcttgta ataacgcatg aacctgttat
     3721 ttcattcttt gagcctttta caggaagata attaacctgt atccatttgg gctcaccccc
     3781 gacgattatt tgtgactcat actccacctc attaccctga aatactttat cgaaattact
     3841 tttaatgtaa tccaattttt ctggagggat acagtcaagt attttattac ccgcctttac
     3901 aggtttaccc caggcccggg caaggttgaa ctctgatggc ttactgatta gtattatttc
     3961 ataatcttta ttgataacat agaattcccc cgcagcactg tccaacactt gttttaattt
     4021 ttcttcttcc tttttgattt cctctgtctt ctcttttatt ttatccgcca gttccttatt
     4081 aaatttcaac aaaaggtcag aggcaagttt tctttgagta acatccctta tcacacatac
     4141 ataaccggca ggtttatcct ctttatcata taaatgcttt accgatatca gtgtttttat
     4201 ctctttgcca tctttagcag aaaagctgat ctctgcctca gaagtgccct ttagacgaag
     4261 ttcacttttc acttcctcag cgatcgattg caaaaaatct gtagatgtaa aatcccggaa
     4321 gttcgctcca acggcttctt tggcctgata gccaaatagt tcctctgctg ccggattcca
     4381 agttaaaata actccttcca tgtcaagaga gataatggca tcccccgttc tttgaaccag
     4441 attcgcaagg tatagtatct ccctttcatc ttcttctctt ttcttcatct ctatacgcac
     4501 ccgcctatat aacattacaa acatcagaat gaataccaag ccaattacaa gaattgtaag
     4561 taccagatta ttggctgctt tttgcctgct atcatttgat tcgtgtagtt ttttgatgtc
     4621 tttatcttca aaagaataaa tgatctgcct gattttgtcc atcactattc ttccctcccc
     4681 atcctgcaca tgtctgttag cggcttcctt gttctgattc ttcatatgat aaatggacag
     4741 ctctgcgaga ttgagttttt gggaaataag ccgctggagt gtatcataca tggattgcct
     4801 ttcaggatat aaagcggata atgatttgat atcaagtgta tcgttagcaa ttctttttat
     4861 agctacaaag taaggagcaa gaaattcttc ctttccggat ataacaaatc ctcttgttgc
     4921 agtttcaata tcctgcatat catcaagaat cacttcaagt atacgaagtg attgtagtga
     4981 ctcatcaacg atggctgtct ccttttttgc cattttcaga ttgacaagac ccagccatcc
     5041 aaaaaatgta attacgacaa atgaaataat aagaccaagg gtaatatttc tggtatagta
     5101 aatacggcgc atttcattcc tttttataag gatgaatggt taatcactaa caggaaaacg
     5161 aattcagagt ccctgagcat taataaaatt aaccaaagct gctgtatttt tcaggtttag
     5221 ttttttaagt atattatacc ggtgtacttc aaccgttttg agtgaaatat cgagtcgtac
     5281 agcaatttcc cgggatgaca acccttcttt tatcaatttt acaatatcca gctcacggcg
     5341 ggacaatgcg ttaagatctg gcttaccatt ctcgtcttca agttcctgct gannnnnnnn
     5401 nnnnnnnnnn nnnnnnnncc tcacagatgt atttcttacc gccattaact tctacaatag
     5461 cattcatcaa ttcatctttg gatgagtttt tcgttacgta gcccatagca ccaatctgca
     5521 tcatttttct ggcataggca ggcatactgt gcatggaaat tccgattact tttgaaccgg
     5581 gtgaatactg gcgtaccagt ttagtggctt caaatccatt gaccggagac atattaatgt
     5641 ccattaaaac aattcggggc tttttttctt ttgctaatga aacaagctct tctgcatcgc
     5701 tggtttcacc tacaaccctg aagcgatcat cgctgtttag aataaaagac catgaatccc
     5761 tgataagttt atggtcgtcg acgaggagga tggttatttt gtccatagga ataattattg
     5821 gtgaattaat ctggataggt tcgtatagaa aattacgaat aaatccttat tcagtaacaa
     5881 aggaaaaaag caatttgcat acgagtaaac gaagaaaatc gagattaagt acttaccctt
     5941 agtaaaagac ctaaaaagat actttctatg acattgcctg tgattattca actacaaggg
     6001 taataaaaaa gcacagccat acttgataca tgactgtgcc aaaaaaagtg gaggcgaccg
     6061 gaatcgaacc ggtgtccaaa catattctct aaaagctttc tacatgttta tcctgttttg
     6121 tattgtcggg aagaaactgg aaaccggcaa ccgattactt ccttagctga atattacttg
     6181 atcaggtggc acagcctata cccgctgcat cttgttttgt tttgagtcgg cggcggggca
     6241 tggaaacaag cgaaccggcc cggcggccct aatgactact taatcactga ttaggcagcc
     6301 atggcatact gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccct
     6421 ttccaattct gaatcctttg tacctgccca ggctgtttac taattattat tgactagcaa
     6481 ctgttgacct gtcattattt cccccatcca aaccaatcgt ttccgttggc atataacatt
     6541 aatcccagta ataataccat acctaccagt tgtgcatact ccagaaactt ctcattaggt
     6601 tttctcctgg ttatcatttc atataaagtg aacaccacat ggccaccatc cagggcaggt
     6661 ataggaagca gattcataaa ggcaagaaca atggacaaaa atgccgtaat gttccagaaa
     6721 gcctcccagc tccatgaagt agggaatatc gatcccatag ctttaaatcc acccacaccc
     6781 ttataagctc ctgttttagg gtttaatatt tttttaaact gatcgatata aaactgcagc
     6841 ttctctcctg cctttcttac accagccgga aaagcttcaa aaaatgcata ctttctttta
     6901 tttatttcat atacacccaa tgctgcatat tgcgcatctt ccaggtaagg tatcccaatc
     6961 tttccttctt cagtaaccct ggaatccagt cttattattt gtccgttccg ttcaacagaa
     7021 agtgaaactt ttccattttt atgctgctgc aatactgcgg ctatttcatc ataaaactga
     7081 ataggtgtac tatcaatagc cacaatcctg tccatttcct gcaaaccggc ttttttacca
     7141 aaagaagtat cttttttatc atatttacca acatacgaag gaatccttgg tgataggagc
     7201 actcctcttt tccttttact ctcaatcagc ttttcgataa gattaacagg aagctcaata
     7261 tcttttttaa caccatttct ttcaaggctt atcgtctttc ctagtaatat tttagcattc
     7321 aaatcttcat agttcctgac agtatcacca ttgacagtga taaccttatc gccattttgc
     7381 aaaccaattt cattcatcag gctatccgtt gtccatatgc catatttcaa tgaagacatc
     7441 ggcgttttct tttctcccca taccatcaaa atcatcgcat aaataacaaa ggccgtcagt
     7501 acattcataa tcacacctcc cagcattaca atcaggcgct gccaggccgg tttgcttctg
     7561 aactcccatg gctgtgccgg ctgtttcatc gcttctttgn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     7681 cactgtatca cctactttct tttttaccaa agcgaaccag gggtcaaaga acaagaagaa
     7741 tttttcaacc ctgcacttaa accatctggc agtaatataa tgcccgaatt catgcaatat
     7801 taccagcaat gacaaagcca gtaataactg ccctgcctgc aagcccacat tagaccaatt
     7861 gatagcgagt aaactcatat ttctatataa aataaaaaat cctgaagctg taagcaggtt
     7921 cagaattccg gttttacaat ttgattatat cggcggcaaa gttacgggcc tccccgtcgc
     7981 tgtcaaaata ttcctccagc gttgggtgtt caataaaggg aacctgctcc attgttttct
     8041 caatcacatt ggtcatatcc aggaagttga tacggttccg cagaaaggca aatacagcta
     8101 tttcattggc agcattcatt atacagggaa gattcccccc cttgaagagg gcatcctgcg
     8161 ccaatgccaa atttctgaat gttttaacat caggctcctc aaatgtgagg gtattcactt
     8221 ttttaaaatc gtatctcgga aaattattgg gtatcctttt tggaaatgcc agtgc
//
LOCUS       DAORLS010000127         8240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4700_length_8240_cov_1.873427, whole genome shotgun sequence.
ACCESSION   DAORLS010000127 DAORLS010000000
VERSION     DAORLS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4700_length_8240_cov_1.873427"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2437
                     /locus_tag="PK977_10525"
     CDS             <1..2437
                     /locus_tag="PK977_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF18597.1"
                     /translation="IVMGVLKPGSNNRIWLSADAVPELSANGKVDHVVLSFTDISEKI
                     AAAFEANTLNRLYKFIGNINELILRDNDRNEIFQQACHTAVQDGGFRMAWFGLYDETT
                     QKVSPFTWAGYEEGYLSSINITADEEATGQGPTGKCIRSKKYQYCDDIATDSDMLPWR
                     DEALKRNYRSSISLPVMVNGKLEAVFTLYMSEPYFFGHPQEIELLLKVTNDIAYALDK
                     LRLAELQMKMEKELHESELMNRAILNAFPDRIFRIKEDGMIIEVQAGVRDQLRPISEL
                     TGKKISEVITKETAEKLMIALHKALVVRKLVSVEYEIAEHGIHFYFEGRIIALSDKEV
                     LFIARDITETKETNATLEKKVKEVSDLRYALDEGTIVDIADQNGVITHVNDNFCAISK
                     YSREELIGQNHRLLNSGHHPSSFFKELWTTVQAGKIWRGEVMEKSRDGSYFWLDTTIV
                     PFINDKGKPYQFVSIRKDITENKMAEEKLIESETKFRSLVEQATVGVFILQGGRFKYT
                     NPGFEKIFGYSREEFLKEVTVEMIVHPQDLEIVKTAIAQKLTGEKISTNYSTRIIRKN
                     GSTAFIEVISSSLIYQGEPASIGTVIDITDKIEEEKRIGKAVNDAQENERLQIGMELH
                     DNVKQIMAASLMSLGFAKDSMNENPVVAAQTIDNVRDYIREAIHELRRLSHQLAPSAD
                     TNLPLSEKIKMLAENMNNANSLDIHINVEDFDEPLRTDIQLAIYRILQEQLSNVLKYA
                     KAKIAEIVIERKGNVIQLRVKDDGKGFDTSVKKDGIGLENIKRRVSVLGGETKIISSP
                     GQGCEIIVRIPLR"
     gene            2589..3962
                     /locus_tag="PK977_10530"
     CDS             2589..3962
                     /locus_tag="PK977_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HRF18598.1"
                     /translation="MISVIIPALNEEKTVGQVVRLAKSSPHVTEVIVVDDKSLDNTIA
                     EAQKEGATVITSTRLGKGASMKDGVLYASNPLIVFLDADITTYQKNIISLLTAPLISD
                     GADFVKSCFSRQAGRVTELVAKPLLSILYPDFPVFWQPLSGMIAGKKEMLEKIEFEEG
                     YGVDIGILIDMYHMGARIEEVSIGHIENDMQPLEQLGKMSREVAAVILKKSRHKEANN
                     FETLEYIQTIREQMEFAIRESSRQLKKVAIFDMDNTLLRASFIHTAAEKFGFKKELLE
                     IVTTQSNPFIRTKQIARLLKGRNISELLKLADEIEVTRGAAEVIELLRLNGFIVGLVS
                     DSYDCITNHFKNRLGFDFSLGNELEFSKSIATGEVKIPSFFLLQEKSMCNHEYCKSHA
                     LAQVCSNFGVPVSDSLVIGDSENDICIIKKAGIGISFCSTNPLLDSIADYVIKEPDFN
                     LLAPVAG"
     gene            4048..5505
                     /locus_tag="PK977_10535"
     CDS             4048..5505
                     /locus_tag="PK977_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRF18599.1"
                     /translation="MGGVFKEFVIRKWLCTLLALTCISWMAGAQLCTGSLGDPVVNIT
                     FGTGGGNTSVYTPPGYSYDASACPNDGYYTITGYTSGCFGNAWHTVNNDHTGGGNFML
                     VNASYQPADFFLYTVRGLCPNTTYEFSAWIMNVLISPTGIQPNLTFRIETPAGAVLNT
                     FNTGDIPVTPVPQWDQYGFYFTTTAGNPDVVLRITNNAPGGIGNDLALDDISFRPCGP
                     VLSSSILGNSNEIDICYYDQPGYTFNAVISPGFLNPVFQWQLSTDSAATWKDIAGATN
                     LVYQRQPTVAGHYWYRLTVAEDGNSGIQSCRIASNVLRVNVRPKPQVNGGPDRIIIKG
                     GSVQLSATATGENIIHTWLPPDGISDVTVLDPVASPAGDTYYTLRATSAYGCVSEDYV
                     MVKVVAGIFVPTAFTPNNDGRNDYWRIPYLDPLQGATVSVFNRYGQLVYQAAGTAVSW
                     DGTFKGIPQNTGTYVYLIQFKDGTPDMKGTFTLVR"
     gene            complement(5561..5989)
                     /locus_tag="PK977_10540"
     CDS             complement(5561..5989)
                     /locus_tag="PK977_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF18600.1"
                     /translation="MKKITLLATAFLFTLAVTAQTKADDVIKVNTVKHDFGKIKQGVP
                     VDFVFEVKNISDKPVVVESTNASCGCTTPEKPTEPIMPGESGKIKVQYNAAAVAPFTK
                     DVYIKLVGIDQPKTVQITGEVLTVEAYEAYLKEKSSKSKN"
     gene            complement(6001..6438)
                     /locus_tag="PK977_10545"
     CDS             complement(6001..6438)
                     /locus_tag="PK977_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF18601.1"
                     /translation="MKKILTLLLLTGCFFLANGQQVQPPDNLQLKESEHDFAQIPQGK
                     PVYYSFEITNTGTAPLKLDDVHASCGCTTPEWSRDPIAPGGTTKIKVGYNAAAEGFFE
                     KFITLTYNGNQTKLIKIKGTVWKAPEGSAPANASINFLKKQTF"
     gene            6561..>7322
                     /locus_tag="PK977_10550"
     CDS             6561..>7322
                     /locus_tag="PK977_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF18602.1"
                     /translation="MENTSPEMIAEAAKLSFDRFREEVLKDFRIACISREASLLGRKE
                     VLSGKAKFGIFGDGKEVAQVAMAKFFKPGDFRSGYYRDQTFMFATGLANVQQFFAQLY
                     ADPDPVNEPFSAGRQMNAHFATRMVDEEGNWLDLAHLKNISSDIAPTAGQMPRGLGLA
                     FASKAFRNVPQLKDFKNLSDNGNEVCFTTIGDSSTSEGHFWETINAAGVMQVPLAVFV
                     WDNGYGISVPKEYQTTKASVSEALSGMQKTPHTNGF"
     assembly_gap    7323..7462
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7463..>7847
                     /locus_tag="PK977_10555"
     CDS             <7463..>7847
                     /locus_tag="PK977_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRF18603.1"
                     /translation="GSHERYKSAERLQWERDWDGIKKMKEWIIANALATEEELNSVAE
                     EAKHHVRECKAAAWEKYITPVKNQVSRAADLINNMAAAIPEQKEVLHKMVVALTAIRE
                     PLRRDVMRTLHAAITLAGNKDAAYWT"
     assembly_gap    7848..7903
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7904..>8240
                     /locus_tag="PK977_10560"
     CDS             <7904..>8240
                     /locus_tag="PK977_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRF18604.1"
                     /translation="SCLYNEGPKSALKVTEVKAHIPVDAPVVNGFEVLNRFFDELFST
                     NPKVMAFGEDLGYIGDVNQGFSGLQQKHGHERIFDTGIRELTIMGQGIGLALRGLRPI
                     AEIQYLDYLL"
BASE COUNT         2420 a         1510 c         1878 g         2236 t
ORIGIN      
        1 gatagtgatg ggagttttga aacccggtag taataacagg atatggcttt cagcagacgc
       61 tgtacctgaa ttgtcggcca acggtaaggt tgatcatgta gtactgtcat ttaccgatat
      121 atcagaaaaa attgctgctg cctttgaagc caatacatta aacaggcttt ataaatttat
      181 tggcaacatc aatgaattaa ttctcaggga taacgacagg aatgaaattt tccaacaggc
      241 ctgtcatact gccgttcagg atggcggctt caggatggca tggtttggtt tgtatgatga
      301 gacaacacaa aaagtaagtc ccttcacctg ggcgggttat gaagagggct atctcagcag
      361 tattaacata accgcggatg aagaagctac aggacaggga cctacaggta aatgtatacg
      421 atctaaaaaa taccagtatt gtgatgatat agccactgac tcggatatgc tgccctggcg
      481 ggatgaagcg ttaaaacgaa attatcgatc ctccatttct cttccggtaa tggtcaatgg
      541 aaagttagag gctgtcttta cgctatatat gtcagagcca tacttctttg gacatccgca
      601 ggaaatagaa ttgttgctca aggttaccaa tgatattgct tacgctttag ataaattaag
      661 gctggcagaa ttgcagatga agatggaaaa agaactgcat gaaagtgagt taatgaaccg
      721 tgccatactg aatgcttttc ctgaccgaat tttcagaatt aaagaggatg ggatgataat
      781 tgaagttcag gcaggggtaa gagatcagtt aaggccaatc agtgagctta ccggaaagaa
      841 aatcagtgag gttataacaa aagaaacagc ggaaaaacta atgattgctc tccacaaagc
      901 acttgttgtg agaaaattgg ttagtgtaga atatgaaatt gcggaacatg gtatccattt
      961 ttatttcgag ggaaggatta ttgccctttc agacaaggaa gttttgttta ttgccaggga
     1021 tattacagaa acaaaagaaa ccaatgcgac actggagaaa aaagtaaaag aggtttctga
     1081 tttgcgctac gcattagatg aaggtactat cgtagacatt gctgatcaga atggcgttat
     1141 tacccacgtc aatgataact tctgcgccat ttcaaaatat agccgtgaag aattgattgg
     1201 gcagaatcac cgtttactta attcagggca tcacccatct tcctttttta aggaattatg
     1261 gacaacagtg caggctggaa aaatctggag aggtgaggtt atggaaaaat caagagatgg
     1321 ttcatatttc tggcttgata caacgatagt gccattcatc aatgataaag gtaagcctta
     1381 ccagtttgtc agtattcgta aggatattac agagaataag atggccgagg agaaattaat
     1441 tgaaagcgaa acaaaattca ggagccttgt tgagcaggcc acagttggag tttttatact
     1501 gcagggtggc cgatttaagt atacaaaccc tggatttgag aaaatatttg gttattcaag
     1561 ggaggagttt cttaaggaag tcacagttga aatgatagta catccgcaag atcttgaaat
     1621 tgttaaaact gctatcgcac aaaagttaac cggtgaaaaa ataagtacta attatagcac
     1681 tcggatcatt cgaaaaaatg gcagtaccgc ttttatcgaa gtaatctcat caagtcttat
     1741 ctatcaggga gaaccagcca gtattggcac tgtgattgat attactgata aaattgaaga
     1801 ggaaaaaaga attggtaaag ctgtaaacga tgcccaggaa aacgaaaggt tacagatagg
     1861 aatggagctg catgataatg taaaacagat tatggctgct tcactcatga gtcttggttt
     1921 tgccaaggac agcatgaatg aaaatcctgt tgtggcagca caaacaatcg ataatgtcag
     1981 agattatatc cgggaagcca tccacgaact gcgaagattg tcacaccagt tggcaccttc
     2041 ggcagatact aatcttccgc tgtcagaaaa gataaagatg ctggcagaaa atatgaataa
     2101 cgcaaattcg ctggatattc atatcaatgt agaagatttt gacgaacctc ttcgcactga
     2161 tatacagttg gctatttatc gtatccttca ggaacaattg tcaaatgttc ttaaatatgc
     2221 aaaggctaaa atagccgaga ttgtaattga aagaaagggt aatgttatac agctcagagt
     2281 aaaggatgac ggtaaaggat ttgatacttc tgtaaagaaa gatggtatag gtctcgaaaa
     2341 tatcaaacgt cgtgtcagtg tactgggagg agaaacaaag atcatttcgt ctccgggcca
     2401 gggatgcgag ataattgttc gaatacctct ccgctaatac tgttgtacct atcgggtatt
     2461 caggataatt cgcaatactt tttgcttgtg atacagctaa ttcattttca gtcagcaaac
     2521 atctaatcag attctaattc ttatcatttc tttttgccgc taactttaat aagttagaat
     2581 accagtgtat gatcagtgtc attataccgg cattaaacga ggaaaaaaca gtaggccagg
     2641 tagtaaggct tgccaaaagt tcaccgcatg ttaccgaagt aatcgtggtg gatgataaat
     2701 ctttggataa tactattgct gaagcacaaa aagaaggagc tactgtaatc acaagcacaa
     2761 gactgggcaa aggggcgtcg atgaaagacg gtgttttata tgcttccaac ccattgattg
     2821 tttttttaga tgccgacata acaacctatc aaaagaacat tatatcatta ctaacagctc
     2881 cacttatcag tgacggggca gattttgtga aatcatgctt ttcaagacag gctggtcggg
     2941 ttaccgagct ggtggcaaag ccattgcttt ccattcttta tccagatttc ccagttttct
     3001 ggcagccgtt aagtggtatg attgccggga aaaaggaaat gcttgagaaa attgaatttg
     3061 aagaagggta tggagttgat atagggattt tgatcgacat gtaccacatg ggtgcaagaa
     3121 ttgaagaagt aagtattggc catattgaaa atgatatgca gccattggag cagttaggaa
     3181 aaatgtcaag agaagtggca gctgttattt taaaaaaatc gagacataaa gaggctaata
     3241 atttcgaaac actggagtat atacaaacca taagagaaca aatggaattt gctataaggg
     3301 aaagctcccg gcaattgaag aaggttgcca ttttcgacat ggacaataca ttgcttaggg
     3361 caagttttat tcatactgcc gcagaaaaat ttgggtttaa aaaagaactt ctggagatag
     3421 ttaccacaca aagcaaccct ttcatccgta caaagcagat agcaagattg ctaaagggca
     3481 gaaacatcag tgagttattg aaactggcag acgaaatcga agtaacaagg ggtgcagcag
     3541 aggtgattga gcttcttcgc ctcaacgggt ttattgttgg cctggtgtcc gacagttatg
     3601 attgtataac caatcatttc aaaaaccggc ttggctttga tttttcatta ggcaacgagc
     3661 tggaattctc aaagagtatt gctaccggcg aagttaaaat cccttctttt ttcctgttac
     3721 aggaaaaaag tatgtgcaat catgaatatt gcaagtccca tgctctggca caggtatgtt
     3781 ccaattttgg agtgccggtt tccgattcgc tggtgatagg cgacagcgaa aatgatattt
     3841 gcattataaa gaaggccggc attggtattt ccttttgctc cactaatcct ttattggatt
     3901 ctatagcaga ttatgtcata aaagaacctg attttaatct gctggcacct gtagccggtt
     3961 gatttttttc atctgttctt aaatacagct catttaaatg gcttgccgaa atggattaac
     4021 ttgcatagct ataattaaaa caacaggatg gggggtgttt ttaaggaatt cgttataagg
     4081 aaatggcttt gtacgttatt ggcattgacc tgtatatcat ggatggccgg tgcacaactt
     4141 tgcaccggca gtctcggaga tcctgtagtg aatattactt ttggaaccgg cggaggaaat
     4201 acttcagttt atacaccgcc ggggtatagt tatgatgcat ctgcctgccc caatgatggt
     4261 tattatacaa ttacaggtta tacttcaggc tgttttggca atgcctggca cacagtcaat
     4321 aacgatcata ccggcggcgg gaactttatg cttgttaatg cttcctatca gccggcagat
     4381 ttttttcttt atacggtacg ggggctttgt cccaatacca cctatgagtt ttctgcctgg
     4441 ataatgaatg tgcttatttc tcccacaggt attcagccca atcttacttt caggattgaa
     4501 acacctgccg gcgctgtact gaatactttc aacacaggcg atataccggt tacgccggta
     4561 ccacaatggg atcaatatgg cttttacttt actactactg caggtaaccc ggatgtggtt
     4621 ttacgaatca ctaacaatgc accgggaggc atcggtaatg atttggccct cgatgatatc
     4681 agtttccggc cttgtggacc ggtactgagt tcatcaatac tgggcaactc taacgaaata
     4741 gatatttgtt attatgacca gcccggttat acttttaatg cagttatttc acccggtttt
     4801 ttgaatcctg tatttcaatg gcagctgagt accgattctg cggctacatg gaaggatatt
     4861 gccggtgcta caaaccttgt atatcaacgc cagccaactg ttgcaggcca ctactggtat
     4921 cggcttacgg tagcagaaga tggaaacagc gggatacaaa gctgtcgcat tgcatcaaat
     4981 gttttacggg taaacgtaag gcccaaacca caggtgaatg gggggcctga caggataata
     5041 ataaaaggag gcagcgtaca gctttctgca acggctacag gcgaaaacat catacatacc
     5101 tggttgcccc cggatggcat tagtgatgtc acagtattag atccggtagc ttcaccggca
     5161 ggcgatactt attacacctt gagggctaca tcggcatacg gatgtgtaag tgaagattat
     5221 gtaatggtaa aagtggtagc aggaatattt gtgcctacgg cttttacacc caataatgat
     5281 ggaagaaatg attactggcg tatcccttac cttgatccat tacagggtgc taccgtcagt
     5341 gtattcaacc gctatggtca gttggtgtac caggctgctg gtacggcagt cagttgggat
     5401 ggaacgttta agggaatacc gcaaaacacc ggaacttatg tttacctgat acagtttaaa
     5461 gacggaaccc cggatatgaa aggaactttt acattggtca ggtaaaaaat aaaaaacctc
     5521 ctgtaaagga ggcttcgcta ttcggttaaa aacgggtagt ttaatttttc gatttactgc
     5581 ttttttcttt caggtaagct tcataggctt ctacggtcaa aacttcacca gtaatctgaa
     5641 cagttttagg ctgatcaata cccaccagct tgatgtaaac atctttggta aaaggagcaa
     5701 cagcagcagc attatactga actttgatct ttccgctttc gccgggcata atgggttcag
     5761 taggtttttc cggtgttgtg caaccgcaag atgcattggt gctttccacc accacaggtt
     5821 tgtcgctgat attctttact tcaaaaacaa aatcaacagg gacgccttgc ttgattttgc
     5881 caaagtcatg cttgacagta tttactttaa taacatcatc tgcttttgtc tgggctgtta
     5941 cagctaaagt aaagaggaat gcagttgcga gtaaggtaat cttcttcatg gtttgttatt
     6001 ttaaaaggtt tgttttttta agaaattaat agatgcattg gcaggggcag agccttcagg
     6061 ggccttccac accgtacctt ttattttgat gagtttggtt tggtttccgt tgtaggtaag
     6121 ggtaatgaat ttttcaaaga acccttccgc ggcagcattg taacccactt tgattttggt
     6181 agtgccaccc ggtgcaatgg ggtcgcggct ccattcagga gtagtgcacc cgcaactggc
     6241 atgtacatca tccagcttta aaggggccgt tcctgtatta gtgatttcaa aactgtaata
     6301 aacgggtttg ccctgtggta tttgggcaaa atcatgttcc gattctttta actgcaggtt
     6361 atccgggggc tgtacttgct ggccatttgc cagaaaaaaa cagccagtta ataataacag
     6421 ggttaaaatt ttcttcatat taccttttga agcttccaaa attagcaaaa agatggcaca
     6481 atacacaggt gggttgcaag gggttacagg tatttttccc cgggggtaat accgcatggc
     6541 aatctcccta tttttgctgc atggaaaata ccagcccgga aatgattgcc gaagccgcca
     6601 aactctcctt cgaccggttc agggaagagg tgctaaaaga ttttcgaatt gcttgtatca
     6661 gcagggaagc cagcctttta ggacgaaaag aagtcctaag tggtaaagcc aaatttggca
     6721 tttttggcga cggtaaagaa gtagcccagg tggctatggc caagttcttt aagcccggcg
     6781 atttccgcag cggttattac cgcgaccaga ccttcatgtt cgcaaccggt ctggccaatg
     6841 ttcagcagtt ttttgcccaa ttatacgcag atcccgatcc cgtcaatgag ccttttagtg
     6901 ccggacggca gatgaatgcc cattttgcca cccgaatggt ggacgaagag ggcaactggc
     6961 tcgacctggc acatttgaaa aatatttcca gtgatattgc tccaacagcc ggccagatgc
     7021 ccaggggact gggacttgcc tttgcttcca aagcttttcg caatgtcccg caactgaaag
     7081 attttaaaaa tctatcagat aatggcaacg aggtttgctt tacaaccatt ggtgactcat
     7141 ccaccagtga gggtcacttt tgggaaacca tcaatgctgc cggggtaatg caggtgcctt
     7201 tagcggtttt tgtctgggat aatggttatg ggatttctgt tcctaaagaa taccagacaa
     7261 ctaaagcgtc cgtttcagaa gctttaagcg gtatgcagaa aacaccccat acaaatgggt
     7321 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnggcagcca cgaaaggtat aaatcagcag aaagattgca
     7501 gtgggaaaga gactgggatg gtattaagaa aatgaaagag tggatcattg ccaatgcgct
     7561 tgccacagaa gaggaattaa attccgtggc ggaagaggcc aagcatcatg taagggaatg
     7621 taaagcagca gcatgggaaa aatatataac gccggttaaa aaccaggtaa gtcgtgctgc
     7681 tgatctgata aataacatgg cggcagccat acctgaacaa aaagaggtat tgcataaaat
     7741 ggtcgttgca ttaactgcca tcagggagcc gttgcgcagg gatgtaatga gaacattgca
     7801 tgcggctatt actttggccg gtaataaaga tgccgcatac tggaccannn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcttgtt tatacaatga
     7921 agggcctaaa agtgccctga aggtaacaga agtaaaagcc catattccgg tggatgcacc
     7981 ggtggttaac gggtttgaag tactgaatcg tttctttgat gaattattca gcacaaatcc
     8041 aaaagtgatg gcttttggtg aagacctcgg ttatattgga gatgtaaacc agggtttcag
     8101 tggtttgcaa caaaaacatg gacatgaaag aattttcgat accggtatcc gtgaactgac
     8161 aatcatggga cagggtattg gactggcgct tcgtggcctg agaccgatcg ctgaaataca
     8221 atatctcgat tatttattgt
//
LOCUS       DAORLS010000128         8139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4771_length_8139_cov_1.821128, whole genome shotgun sequence.
ACCESSION   DAORLS010000128 DAORLS010000000
VERSION     DAORLS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_8139_cov_1.821128"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PK977_10565"
     CDS             complement(<1..865)
                     /locus_tag="PK977_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRF18605.1"
                     /translation="MGIPFDGITGTNNAITDVPGIEVGYTTIIKDSGKLIAGKGPVRT
                     GVTVILPKGKKEESYPAAWFSLNGDGEMTGISYIEDYGMGYGPIGITNTNSVGIVKDA
                     IGQWCFKKFSKGDFVDFSFGLPVAAETWDGMLNDINGFHIKMEHVWKALDSAKGGKIA
                     EGNVGGGTGMVLYEFKGGSGTASRTFKIDTNTYTLGVFVQSNFGGRKELTISGVPVGK
                     EISGYEPVIHEPKRKDGSIIVIIATDAPLMPLQLKQVAKRATHGIARTGTYSHNSSGE
                     IFLALSTAAPSY"
     gene            complement(1005..>1760)
                     /locus_tag="PK977_10570"
     CDS             complement(1005..>1760)
                     /locus_tag="PK977_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF18606.1"
                     /translation="QKYNVLYMHDGQMLFDSATTWNKQSWEVDDVITGLMQQQKIKDV
                     IVVAIWNGGTTRHADYFPQKPFEALSQAEKDTVYSAARSNGVSVFNNYKIQSDNYLKF
                     IVKELKPFIDKKYSVYKDRSHTFITGSSMGGLISMYAICEYPKVFGGAACLSTHWPGI
                     FTMENNPVPNAFFSYLKKHLPSPKTHKIYFDYGDATLDALYPPLQQKADATLKQRGFT
                     SANWLTKYFPGEEHSEKAWHKRLAVPLTFLLGR"
     assembly_gap    1761..1791
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1959..>2728)
                     /locus_tag="PK977_10575"
     CDS             complement(1959..>2728)
                     /locus_tag="PK977_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HRF18607.1"
                     /translation="GYRCDVAGFIPVDFWENARKELDAIKPVFMLAEWESRDLYKRSF
                     DMTYSWSLWDKMHQATVGKKGAGILTEYMAHDVGSVPKDAYRMTFTDNHDKNSWEGTQ
                     YSNFGDGLQACMVFCTVVNGMPMVYGGQEAGLNKSLKFFDKDTIEWKDHPNAALYKSL
                     FKLKHENQALWNGGWGGEMVRIFNDKPNEVISFAREKNEDKIISVINFSDNPVTVKLN
                     SKFQKGNYTDWFTNTAFEIKGDDVFTLQPWGYFVLVK"
     assembly_gap    2729..2746
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2747..3355)
                     /locus_tag="PK977_10580"
     CDS             complement(<2747..3355)
                     /locus_tag="PK977_10580"
                     /inference="COORDINATES: protein motif:HMM:NF026052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF18608.1"
                     /translation="MKKIYPVIAAAILFSALISCNTDKSTKESTADPYTPKSYVELKH
                     PEWSKNATIYEVNIRQFTPEGTFKAFEAHLPRIKDMGIDIIWLMPIHPIGEKNRKGTL
                     GSEYSVKDYLGVNPEYGTMDDFKALVKKIHDMGMYVILDWVANHSAWDNQLATEHPDW
                     YTKTPEGEFQPTPWYDWSDVIDFDYNVPGMRKYMTDALKFWVK"
     gene            complement(3431..>3782)
                     /locus_tag="PK977_10585"
     CDS             complement(3431..>3782)
                     /locus_tag="PK977_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18609.1"
                     /translation="STDLLIGVSIGLLISIYFIVQNNFKDEFKITHHKKHETEVYEIK
                     LHSNVTFLNKVRLRNTLDKIPEYSILTIDGRDCSFIDYDILEIISEYANKAHDRHIEL
                     HLEGIEKVNITAVH"
     assembly_gap    3783..3807
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3808..>4792)
                     /locus_tag="PK977_10590"
     CDS             complement(<3808..>4792)
                     /locus_tag="PK977_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF18610.1"
                     /translation="VSGPAAGLTTLVAASIISLGGYPVFLLAVIVAGLFQLLLGVLKA
                     GIIANYFPSSVIKGMLAAIGILLISKQIPLALGYDQPDFWTSGFLQLFSSGKISSNFQ
                     TLNQHITKGAIIIALVSLAILIIWQRPYAKKLRLIPAPLAVVVAGVLLHFIFLSWMPG
                     FALKTNQLVNIPENVFASIRFPDFTKLFSNIEIWKAGVLIGLLASLETLLCVEAIDKL
                     DRHNRITPVNRELVAQGVGNIACGLLGAIPMTAVVVRGAANADAGARTKLSAFTHGVF
                     ILLAVLLIPFLLNYIPLASLAAILIVTGYNLTKPKLYRNMWSLGLKQLLPF"
     assembly_gap    4793..4817
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5268..5277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5278..5729
                     /locus_tag="PK977_10595"
     CDS             <5278..5729
                     /locus_tag="PK977_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF18611.1"
                     /translation="TAMGLLHNKEDAEEITQDVFLKVYDSLSSFNQRATLSTWIYRVT
                     INTCLNFLKKKKGNRLLVGLSALLGLASKEKQCEKLVTDKEEKDSIRKAMQELPGKQR
                     LAFVLTKYEELPQRQVAEIMQISEGAVEQLVIRARNNLRKKLEKIVG"
     gene            5781..6089
                     /locus_tag="PK977_10600"
     CDS             5781..6089
                     /locus_tag="PK977_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18612.1"
                     /translation="MKQKMNIDEMIDELIKEERNEEFNPFLATRVMAAVQERQYQKEK
                     KINPAWKTVMVAAGITVAFFLGISIGSLYESESRNDGIMLSNDGAMENFGFYSQIGEE
                     "
     gene            6095..6619
                     /locus_tag="PK977_10605"
     CDS             6095..6619
                     /locus_tag="PK977_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18613.1"
                     /translation="MKRKTQILTWAVVLLLITNSVTIATILYHNYRERKNQENITITS
                     SGGTNMINGRFLRQELGFDQTQMDVFRQANQEFRPTAMFITDNIDSLKNEMFVELREI
                     KPDTVKLDNLSEMIGQLHGQLKQETYTFYLRLKNICSPEQQAELEKAFLPLFKNERMT
                     TLPGQHRRKGWNRD"
     gene            6647..6931
                     /locus_tag="PK977_10610"
     CDS             6647..6931
                     /locus_tag="PK977_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18614.1"
                     /translation="MKKMIFTAIVMIAMSGLLSAQTTRPGNGNPGTNKNNPAYVDANK
                     NNVCDNYENGNRSCVRNGQGQGRRVGNGYGMGNRQGRQFGQCRRGGGRRS"
     gene            7018..7524
                     /locus_tag="PK977_10615"
     CDS             7018..7524
                     /locus_tag="PK977_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF18615.1"
                     /translation="METVIRQWKNEDLQELVSQANNINVWNNLRNYFPHPYTEEHGKA
                     WLEKVVDALPAINMAIEADGKLAGGIGLILNGDVYIKSAEIGYWLGEQYWGKGIATEA
                     VRQMTEYAFYYFDLVRLYAEVFETNKTSMRVLEKNGYYLEGVRRKAVFKNDVLMDDYI
                     WVKLKTIA"
     gene            7548..7790
                     /locus_tag="PK977_10620"
     CDS             7548..7790
                     /locus_tag="PK977_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRF18616.1"
                     /translation="MYLKKNIATSESGFIFNPSTGDSYAANQVATDILQLLKQGTALS
                     LVKEQLLEKYRVDPLRLEQDWDDFINQLKQANLLES"
     assembly_gap    7858..7867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7868..>8139
                     /locus_tag="PK977_10625"
     CDS             <7868..>8139
                     /locus_tag="PK977_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18617.1"
                     /translation="DSPGPGIPVIRGLREGLKRPLRIIGLSYETLEPGIYMHDVVDKT
                     YQVPYPAAGSESLLKRLVYIHEKENLQLIIPNFDAELHNFIKLAPV"
BASE COUNT         2450 a         1629 c         1681 g         2260 t
ORIGIN      
        1 tgtaggatgg agcagcagtt gacaaggcca ggaaaatttc gccggaactg ttatgagagt
       61 aggttcctgt tcttgcaata ccatgggtag cccttttcgc aacctgcttt aattgtaatg
      121 gcattaaagg agcgtcagtt gcaattataa caataataga gccatccttt cttttgggtt
      181 catgtattac cggttcataa ccactgatct cttttccaac cggcacaccc gaaatagtca
      241 gctcttttct tccgccgaaa ttggactgca caaatacgcc tagtgtataa gtgtttgtat
      301 caattttaaa tgtcctggag gctgtgccac tcccaccttt aaactcatat aataccattc
      361 cggtgccacc accaacattt ccttcggcta ttttgccccc ttttgcactg tcaagtgctt
      421 tccatacatg ttccatttta atatgaaatc cattgatatc attcagcatg ccatcccagg
      481 tttctgcagc aacaggtagt ccaaatgaaa agtcgacaaa atcacctttt gaaaattttt
      541 tgaaacacca ttgtccgata gcatctttta ctataccaac actgtttgta ttggttatgc
      601 caatgggtcc ataacccatc ccataatctt ctatatagga tattccggtc atttcaccgt
      661 caccattcag tgaaaaccat gctgcaggat agctttcctc ttttttccct ttgggcagta
      721 taactgttac cccggttctt accggtcctt tgccagctat cagtttgccc gaatctttga
      781 taatcgtagt atagcctact tcgatacccg gaacatcagt aatagcattg tttgttccgg
      841 ttattccgtc aaatggtatc cccaaatccc gggctctctt ctttataact gtttgcgcag
      901 acgaagaaat gccgaggcat aagatacaca gccaggtaaa aattgttttg caaaattcat
      961 cttggtagta aataggtatt aataaaggta caaaatgaaa aagcctacct gcccaataaa
     1021 aacgttaaag gaacagccag ccgcttatgc catgcttttt cactatgttc ttcaccggga
     1081 aaatattttg tcagccagtt ggcagaagta aatccccgtt gtttcaaagt agcatcagct
     1141 ttttgctgaa ggggaggata taaggcatcc agtgtagcat ccccgtagtc gaaatatatc
     1201 ttgtgtgttt ttggactggg caaatgtttt tttagataac tgaaaaaagc attgggcacg
     1261 ggattatttt ccatggtgaa gatacccggc caatgtgtcg agagacaggc tgcgccacca
     1321 aatactttgg ggtattcgca aatagcatac attgagatta gtccgcccat gctgctgccg
     1381 gtaataaaag tatgcgaacg gtctttgtat acactgtatt tcttgtcaat aaaaggtttt
     1441 agttctttta ctataaactt cagataattg tcagactgaa tcttgtaatt attgaaaact
     1501 gaaacaccgt tgctccgggc agctgaatac acagtgtcct tttctgcctg tgataatgcc
     1561 tcaaatggtt tttggggaaa ataatcagca tgtcttgttg tgcccccgtt ccagatggcg
     1621 acgactatga catcttttat tttctgttgt tgcataagtc cggtgataac atcatccacc
     1681 tcccatgatt gtttgttcca ggtagtggcg gagtcaaaca acatctggcc atcatgcata
     1741 tacagcacat tgtacttttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagccgtcag
     1801 gcaaccaaac atccacatga cgggctgtta catattttga tggaaagttt tcatgtcgtt
     1861 gcaggctgcc actggcaaca acaggtaact gtgctgcagc aagtagcccc aaaaagcaaa
     1921 tgaataatgt gattaaaaaa tgtttcatta aaacggcttt actttactaa cacaaagtag
     1981 ccccatggtt gaagagtgaa tacatcatct cctttgattt caaaggctgt gttggtaaac
     2041 cagtcggtat agtttccttt ctggaatttt gaattcaatt taacagtgac agggttatcg
     2101 ctaaagttta tcactgaaat gatcttgtct tcattctttt ctcttgcaaa cgaaatcacc
     2161 tcattgggct tatcattaaa tatccgtacc atttcacctc cccatccgcc gttccataaa
     2221 gcctggtttt cgtgttttaa tttgaacagg gatttgtaca aagccgcatt gggatgatct
     2281 ttccattcaa tagtgtcttt atcaaaaaac ttaagcgatt tatttagtcc tgcttcctgg
     2341 ccaccatata ccatcggcat gccatttact acagtacaaa ataccatgca ggcttgcaga
     2401 ccatcaccaa aattggaata ttgtgtgcct tcccatgaat ttttatcatg attatctgtg
     2461 aaggtcatcc ggtatgcatc cttaggtaca gagcctacat catgtgccat atattccgta
     2521 agaataccag ctcctttttt tccgactgtg gcctggtgca ttttatccca caggctccat
     2581 gaataagtca tatcaaagga tcttttgtac agatctcttg attcccattc agccagcatg
     2641 aatacaggtt tgatcgcatc cagttctttg cgggcatttt cccaaaaatc aacgggaata
     2701 aaaccggcca cgtcacaacg ataaccgtnn nnnnnnnnnn nnnnnncttt acccaaaact
     2761 tcaatgcatc ggtcatgtat tttctcatgc ccggaacatt gtaatcgaaa tctattacat
     2821 cgctccaatc gtaccagggt gtcggctgaa attcaccttc cggtgttttg gtataccagt
     2881 cgggatgttc cgtagctaac tgattatccc aggctgaatg atttgccacc cagtcaagga
     2941 tcacatacat acccatatcg tgtattttct tcaccagtgc tttaaaatca tccattgttc
     3001 cgtattcggg atttacaccg aggtaatctt tcaccgagta ttcacttcca agtgttccct
     3061 tcctgttttt ctcaccaata ggatggatag gcatcagcca gataatatca atgcccatat
     3121 cttttattcg gggaaggtgt gcttcaaaag ctttgaacgt tccttccggg gtaaactggc
     3181 ggatattcac ttcatagatg gtagcattct tgctccactc aggatgtttc agctctacat
     3241 aggatttagg agtataaggg tcagcagtac tttcttttgt tgatttatcc gtattgcaac
     3301 tgatcagtgc actgaaaagg atcgccgcag ctattacagg gtatattttt ttcataccgg
     3361 agttttaatg caacggaagg tacactgttt cgattattca gtaaatgact gtttaaacaa
     3421 aagatgagcc ttagtgaact gcagttatat ttactttttc tattccttca aggtgtagtt
     3481 caatatgccg gtcatgggcc ttgttggcat attcgctgat gatctccagt atatcgtagt
     3541 caatgaaact acagtcacgg ccatcaatag tcagtatgct gtattcgggt attttatcca
     3601 gtgtattacg aagcctcact ttgttcagaa atgtaacatt gctgtgcagt ttgatctcat
     3661 acacttcagt ttcatgtttt ttgtgatgag tgattttaaa ctcgtctttg aaattattct
     3721 gcacaataaa ataaatcgat atcagcaggc caatgctgac accaatcaat aaatcggtag
     3781 aannnnnnnn nnnnnnnnnn nnnnnnnatg aaaggcagca attgtttcag tcccaggctc
     3841 cacatgttac gatagagttt gggtttggtc aggttataac ctgttacaat cagtatggcg
     3901 gccaatgaag ccaggggaat atagttaagt aaaaaaggta tcagtagcac ggccagtaat
     3961 atgaatacac catgtgtaaa tgcagaaagt ttggttctgg ctccggcatc tgcattagca
     4021 gcacctctta ctaccacagc tgtcataggg atggcgccca ataaaccgca ggcaatatta
     4081 cctacaccct gtgctaccag ttcacggttc accggtgtga tgcgattgtg ccggtccagt
     4141 ttgtcaatag cctctacaca caataatgtt tcaaggctgg ccagtaatcc tatcaagaca
     4201 ccggccttcc agatctcaat attggaaaag agttttgtaa aatcaggaaa ccggatactc
     4261 gcaaaaacat tttcaggaat attaaccagt tggtttgttt ttaaggcaaa gcccggcatc
     4321 cagctaagga aaataaagtg aagtaaaaca cctgcaacaa caacagccag cggcgcaggt
     4381 atcaatctta atttctttgc atacggacgt tgccatataa ttaaaatggc caatgaaacc
     4441 aaagcgatga taattgctcc tttggtgatg tgctggttca atgtttgaaa attgctgctg
     4501 atctttccgg aagaaaataa ttgtaaaaaa ccgctggtcc agaaatcggg ctgatcatag
     4561 cccaatgcca gcggaatctg tttggagata agcagtatac cgatagcagc cagcattcct
     4621 tttattactg atgaagggaa atagttggca ataatacctg ctttcaatac gcctaatagc
     4681 aactggaaca ggcctgctac aataacggcc aataaaaaaa caggataacc acccaatgaa
     4741 ataatagaag ctgctaccaa tgttgttaat cctgcggcag ggcccgatac acnnnnnnnn
     4801 nnnnnnnnnn nnnnnnncaa caaggccacc aataataccg gaaagaagac cggcatatac
     4861 cggcgcaccg gatgccagtg caatacccag acaaagggga agggctacca aaaagacaga
     4921 gaggcccgca ggcagatcat gcctgaacca catcagccgg tagtacttca tcgttcgcct
     4981 cacaataaat aaagagttgc aggagacgca aagataaatt tttaatatcg tttaaatgcc
     5041 agtagacacc gattaaagtg ataaaagtta cagatcagca tgatataagt tttatctttt
     5101 acaagtgtcg tcatagcaac tgagcctgct attgtataaa attactcccc caatagctgt
     5161 aataattatc gccggtgatg gacgagtttt cgttgataca aaatattctt gacggtagtc
     5221 aggctgactt tgagcagctg gtaaagatgt atcagccgaa cgtatttnnn nnnnnnngga
     5281 cggctatggg tcttctgcac aataaagaag atgcagagga gatcacacaa gatgtttttc
     5341 tgaaagtata tgactctctt tcttctttca atcaaagggc aacattatct acatggattt
     5401 accgggttac aataaataca tgtctcaatt tcttgaaaaa aaagaaagga aacaggttat
     5461 tggttggctt atctgcttta ttagggctgg cttccaaaga gaagcaatgt gaaaaactag
     5521 ttaccgataa agaagaaaaa gattcaatac gaaaagctat gcaggagctt ccgggaaaac
     5581 aaagactggc atttgtattg acaaaatatg aagagcttcc tcaaagacaa gtagctgaaa
     5641 ttatgcaaat aagtgaagga gctgttgagc aactggtgat cagggcacgg aacaacctta
     5701 gaaaaaaact ggaaaaaata gtgggttagc ccataggatt atttattaac ctgttgtcta
     5761 attaataaag cagtcaaaga atgaaacaaa agatgaacat agatgaaatg attgacgagc
     5821 tgataaagga agagagaaat gaggagttca acccattttt agctacaaga gtgatggctg
     5881 ctgtacagga aaggcaatat caaaaagaga aaaaaatcaa cccggcctgg aaaacggtaa
     5941 tggttgctgc cggaattaca gtggcctttt tcctgggtat ttctattggc agcttatatg
     6001 aatcagaaag cagaaatgat gggataatgc tgagtaatga cggagccatg gagaattttg
     6061 gattttattc tcaaatagga gaagaatgat aacaatgaaa cggaaaacac aaattttgac
     6121 atgggcagtg gtgttgctgc taataaccaa ttcagttaca atagctacga ttctgtatca
     6181 caattacagg gaaagaaaga atcaagagaa catcactatt acatcttccg gaggcactaa
     6241 tatgattaac ggaaggtttc ttaggcagga attgggcttt gatcaaacgc aaatggatgt
     6301 atttcgccag gctaatcagg aatttcgccc cacagcaatg tttattacag ataatattga
     6361 ttctttaaag aatgagatgt ttgttgaact cagagagatt aaacctgata cagtaaaact
     6421 tgataacctg tccgaaatga tcgggcaatt acatggacaa ttgaaacagg agacctatac
     6481 tttctaccta cgattgaaaa atatctgttc tccggaacag caggcagagc tggaaaaagc
     6541 atttctgccc ctatttaaaa atgaaagaat gactactctc ccgggccagc accggagaaa
     6601 aggctggaac agggactagc aattattaac agttaaatat tgaccaatga aaaagatgat
     6661 tttcacagca atcgtcatga ttgctatgag cggcttattg tctgctcaaa ctacaaggcc
     6721 cggaaacggt aatcccggca ctaacaaaaa caatcccgcc tatgtagatg ctaataagaa
     6781 caatgtgtgt gataactatg aaaatggaaa caggtcatgt gtccgcaacg gacaaggcca
     6841 gggccgcagg gttggaaatg gctatggaat gggaaacagg cagggccgcc aatttgggca
     6901 atgccgcaga ggcggtggaa gaagatcata agctggtcgc tgaagttttc tgacagtaac
     6961 agttaatagc cggcaaatga agaaaaaccg ggtgaggaac agttcaattc ttcttttatg
     7021 gaaacagtaa tacgacaatg gaaaaatgaa gacttgcagg agctggtatc ccaggcaaac
     7081 aatataaatg tgtggaataa ccttcggaac tactttccgc atccctacac agaagaacat
     7141 ggcaaagcct ggctggaaaa agtggtggat gctttgccgg ctattaatat ggctattgaa
     7201 gcggatggaa aactggctgg cggtatcggc cttatcctga atggtgatgt gtacatcaaa
     7261 tcagctgaaa ttggttattg gctgggagaa caatactggg gtaagggtat cgccacggaa
     7321 gctgtacggc aaatgacaga gtatgctttc tattatttcg atcttgttcg tttatatgca
     7381 gaagtgtttg aaacgaataa aacgtcaatg cgggtactgg aaaaaaatgg ttattaccta
     7441 gagggtgtca ggcgcaaagc cgttttcaaa aacgatgtac tgatggatga ttatatctgg
     7501 gtaaagctga aaactatcgc atgagtaatt ctttttaaaa ctaagttatg tatctcaaaa
     7561 aaaatattgc caccagtgag tcagggttca tcttcaatcc ttcaaccggc gattcttatg
     7621 ctgctaacca ggtagccaca gacattcttc aattgttgaa gcaaggaaca gctctttcat
     7681 tggtaaaaga gcagctgctt gaaaaatatc gggttgaccc gctgcgcctt gaacaagatt
     7741 gggatgattt tattaaccag ctaaagcagg ccaacctgct tgaaagctga tcaaatagtt
     7801 ttgtatgaaa aagcctgatg acctgttaac cattgctgtt acgggtctga atgcaatnnn
     7861 nnnnnnngat agtcccggtc ccgggatacc ggtgatacgg ggattaagag aaggacttaa
     7921 gcggccacta agaataattg ggttgtctta tgaaacgctt gaaccaggga tctatatgca
     7981 tgatgttgta gataaaacct accaggttcc ttatccggca gcaggatcag agtcattgct
     8041 aaaaaggctc gtttatattc atgaaaaaga gaatttgcaa ctcatcattc ccaattttga
     8101 tgcagagctg cacaacttta tcaaactggc cccggtatt
//
LOCUS       DAORLS010000129         8067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4818_length_8067_cov_1.682227, whole genome shotgun sequence.
ACCESSION   DAORLS010000129 DAORLS010000000
VERSION     DAORLS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4818_length_8067_cov_1.682227"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PK977_10630"
     CDS             <1..373
                     /locus_tag="PK977_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18618.1"
                     /translation="IYNTLTDHVKRVTKANDLSEPNGDSPVGQGERIGISYDGSWIAY
                     NTNATNLGVPKGNIVIQSTQDGRIIPITTSTYGSTARPMLSRYGNYVTAGCSEKYDQR
                     FPSSGIFVIYTDKGPCISCNE"
     gene            416..>881
                     /locus_tag="PK977_10635"
     CDS             416..>881
                     /locus_tag="PK977_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18619.1"
                     /translation="MISKKIVLYQSLLLILLPSLVFAQSETYDLVTFTPPAGWKKESK
                     ETIITYSQINQQTGSWCMISVVKSTVSKGTINADFSSEWNELIVKPYQASASPENNEV
                     TEADGWKIKAGGGKFTFNKADAIALLTTFSGYNRCASIISLTNSQEYLQTIQD"
     assembly_gap    882..1067
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1068..>1350
                     /locus_tag="PK977_10640"
     CDS             <1068..>1350
                     /locus_tag="PK977_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18620.1"
                     /translation="HYPKEGTIFPADPEPLTNAAWNILVAPRYSNLKNYKTAYISTYN
                     RPYLGFGNATENASGKDVFIVFFRQGETGWIEFVIPDKSTFIQSFGFDPY"
     assembly_gap    1351..1377
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1378..1809
                     /locus_tag="PK977_10645"
     CDS             <1378..1809
                     /locus_tag="PK977_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18621.1"
                     /translation="NTDLMNALISMSNYNKFAIAASDFTGTWTSDFSGVQQLYSVYTG
                     QYAGMNINQSNETFQFGTGNTYNWKLLAVNGMVGNMKYAEVKSAGKFTVLNNWQISFS
                     KIENKAKKYHAYFKCIKGARLLMLLDADYPGSGIYTAYGKK"
     gene            1840..2784
                     /gene="fmt"
                     /locus_tag="PK977_10650"
     CDS             1840..2784
                     /gene="fmt"
                     /locus_tag="PK977_10650"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRF18622.1"
                     /translation="MRDYRSLRIVFMGTPEFAVASLNALVEAGCNIVAVITAPDKPAG
                     RGMKLTESVVKKYAVENGLKVLQPEKLKNPEFLTALKSLNADLQIVVAFRMLPEAVWN
                     MSPMGTVNVHGSLLPQYRGAAPINWAIINGEKETGVTTFKLKHEIDTGDILLQERFPI
                     GDDETAGEVHDRMKEIGAKILVETVKGLAEGSLTEQPQTLELKPQTLVHAPKIHTDTC
                     IIHWDRPVETVHNLIRGLSPYPGAFTILNEKMLKVIRSKKEITYPKSTEGDYETDGRT
                     FLKFACSDGYIHLLEVQLEGKKRMSITDFLKGYRFNGN"
     gene            complement(2781..3758)
                     /locus_tag="PK977_10655"
     CDS             complement(2781..3758)
                     /locus_tag="PK977_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF18623.1"
                     /translation="MKKLFLFVLSFVLCAWHLAAQKGDLMIKKGDKGLYLEHKVAPKE
                     SFFAIGRLYNVHPRFIASYNKLDISKGLQIEQKIRIPLTDTNFTQNGGKGTPVYYRTG
                     EKEGLMSVSNKYNKVSLANLRWWNNLSSDELKKDTKLIIGFLLSSEMKSITVSGSPKN
                     DQPVAEEKKEKQPDVTEEADLTKKEVKKVAEDEEKAEKKEEKKDPPPVVKEVRKVTIE
                     GQGYFKNHFEQQSKLSAPSKDETVTASIFKTLSGWEDGKYYLLIDNVQPGTIVRIINP
                     SNNKAIYAKVLGEMSGIRQNQGLDIRISNAGAAALDITEQDKFIVKVNY"
     assembly_gap    3877..3969
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3970..>4319)
                     /locus_tag="PK977_10660"
     CDS             complement(<3970..>4319)
                     /locus_tag="PK977_10660"
                     /inference="COORDINATES: protein motif:HMM:NF028436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HRF18624.1"
                     /translation="IACFQKKEGDVYVAPKIKEKVESITIKEKAIVNNWVKADGLSFI
                     KHEDTVYAWPEILYNDFNLLLAHLRVVYSGVLAGELMREKLIPDHALAMSGLVADSVG
                     KIELTYDDAIKYLQ"
     assembly_gap    4320..4347
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4348..>4706)
                     /locus_tag="PK977_10665"
     CDS             complement(<4348..>4706)
                     /locus_tag="PK977_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18625.1"
                     /translation="NNDPKEFARLSNYFDVIIVDAPCSGSGLFRKDPEAIAEWSENNV
                     ALCSQRQQRILADVLPALKKDGILIYSTCSYSRQEDEEIVDWLNKEFSVAPVALSVDP
                     GWGITISDGGYRFWPDKV"
     assembly_gap    4707..4795
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5066..5208
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5209..5985)
                     /locus_tag="PK977_10670"
     CDS             complement(<5209..5985)
                     /locus_tag="PK977_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRF18626.1"
                     /translation="MFVMPIGGADMPVVISLLNSFTGVAAATGGFLYNNKAMLTGGIL
                     VGAAGTLLTILMCKAMNRSLTNVLIGSFGGSKAGPAGQKQQGAVKEITLSDAAVVMAY
                     ANKVMIVPGYGLAVAQAQHACHELEKLLESKGVNVRYAIHPVAGRMPGHMNVLLAESD
                     VPYEKLLEMEQANEEFATTDVVLILGANDVVNPAAKTDPASPIYGMPILDVELAKNVV
                     VNKRSMKPGYAGIENDLFFRPKTSMLFGDAKKVLQDLVSEI"
     assembly_gap    6057..6066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6067..6629)
                     /locus_tag="PK977_10675"
     CDS             complement(<6067..6629)
                     /locus_tag="PK977_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRF18627.1"
                     /translation="MELNILTLCYIIASVTFIVGLKMLSNPAKARNGNLVAAAGMALA
                     IFATIFLYEDGGEKLGNYAWIFGGIIIGSVIGTLAAKKVKMTAMPEMVSLFNGMGGAC
                     AALISAAEFNHLYQHYKGPMDIPYSSVMPVGEYITIIAGAIIGSVSFAGSVIAWGKLN
                     GRIKDFAFKGQHLVNIFILLLVLAAAV"
     gene            complement(6639..6950)
                     /locus_tag="PK977_10680"
     CDS             complement(6639..6950)
                     /locus_tag="PK977_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRF18628.1"
                     /translation="MDSLLNWITANQQIIYIVILMIFVGIEVIGHVPSVLHTPLMSGA
                     NAIHGVVIIGAIIVMGKAESDNYLALIIGFLAVILGTLNVVGGFVVTDRMLEMFKKKK
                     K"
     gene            complement(6955..>8067)
                     /locus_tag="PK977_10685"
     CDS             complement(6955..>8067)
                     /locus_tag="PK977_10685"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRF18629.1"
                     /translation="IMTIGLLKEPAPETRVSLLAEAVGTLTKKGINVLIEDGAGEKAF
                     CNNTEYKKAGAQIKSRNEVLASSDIILSIHQPGLSEVTNLQSKVLIGVYQPLFHVELM
                     KQWASAGVTTFSLDMLPRTTRAQAMDVLSSQANIAGYKAVLLAANTYGRYFPMFMTAA
                     GSIAPAKVLILGAGVAGLQAIATARRLGAVVEVFDTRPAVKEEVMSLGAKFIEVEGAA
                     DASKAGGYAVEQSEEFMQRQKAKIAESAAKADIIITTAQIPGKKAPLLITEEMIGAMR
                     NGSVIIDLAAATGGNTPFTKNNETVVVNGVSIVGNSFLQSTMPSDASKMYGKNVLNFL
                     QLIITKEGAINLNWDDDLVKGCCITHNAEIIHERLK"
BASE COUNT         2235 a         1669 c         1417 g         2170 t
ORIGIN      
        1 tatttataat accttgaccg atcatgtaaa acgggtaacc aaagccaatg acctttctga
       61 accgaatggt gacagcccag tcgggcaggg tgaacgtatt ggcatcagtt acgatggcag
      121 ctggattgct tataatacca atgccaccaa tcttggtgtt ccaaaaggaa atattgtaat
      181 tcaaagtaca caggacggga gaataatacc aataacaaca tcaacttacg gaagtacagc
      241 cagaccaatg ttatcaagat atggaaatta tgtaacagca ggctgcagtg aaaaatacga
      301 tcagcggttt ccttcgtccg gcatttttgt gatttatacc gataaaggtc cttgtattag
      361 ttgtaatgaa tagttttcac aaccctgtaa aattgaatat ttaaaccttc aaactatgat
      421 ttcaaagaaa atagtgttgt atcaatcatt attactgata ttactcccat cattggtatt
      481 tgctcaatcg gaaacttatg acctggtaac ttttacacca ccggcaggct ggaaaaaaga
      541 atctaaagaa actatcatca catacagtca aataaaccag caaaccggca gttggtgtat
      601 gatatctgtt gtaaaaagca ctgtaagcaa aggcactata aatgcagatt tttcaagcga
      661 gtggaatgaa ttgattgtaa aaccttacca ggcctccgcc tcacccgaaa ataacgaagt
      721 aacagaagct gatggctgga agataaaagc gggtggcgga aaatttacat ttaataaagc
      781 tgatgctatt gcattgctta cgacattcag tggttataat cgttgtgcaa gtatcatttc
      841 gcttaccaac agccaggaat acttacagac tatacaggat tnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca ctatccgaaa
     1081 gaaggaacaa tttttccagc agatcctgaa ccgctgacaa atgctgcctg gaatattttg
     1141 gtagctccca ggtacagtaa tctgaaaaat tacaaaacag catatatcag tacctataac
     1201 aggccttatc ttggatttgg gaatgctaca gagaatgcct ctggtaaaga tgtttttatt
     1261 gttttcttcc ggcagggtga aaccggatgg attgagtttg taatacccga taagagcaca
     1321 tttatacaaa gctttgggtt cgatccttat nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
     1381 actgatttaa tgaacgcatt gattagtatg agtaattaca acaaatttgc tattgctgct
     1441 tctgatttca ccggtacatg gaccagtgat ttttccggtg tacaacaact ttacagtgtt
     1501 tacacggggc aatatgccgg catgaatatt aaccagtcta acgaaacctt tcaatttggc
     1561 acaggcaata cctacaattg gaaactgctg gctgtaaacg gaatggtagg aaatatgaaa
     1621 tacgctgaag taaaatctgc cggaaaattt acggtgctta acaactggca aataagcttt
     1681 tcaaaaattg aaaataaggc aaaaaaatac catgcctatt ttaaatgtat taaaggagcc
     1741 aggttattga tgctgctgga tgctgactac ccgggcagcg gaatttatac agcctatggt
     1801 aaaaaataaa aaaagggttt agttaccttc gcagtaagta tgagagatta ccggtcactg
     1861 cgaattgttt ttatgggcac tcccgagttt gctgtagcat cattaaatgc actggtggaa
     1921 gccggatgca acattgtcgc agtgattact gcacccgata agcctgctgg cagagggatg
     1981 aaactgaccg aaagtgttgt aaaaaaatac gctgttgaga acggactaaa agtgctgcaa
     2041 cctgaaaaat taaagaaccc ggaattttta actgccttaa aatctttaaa tgccgatctg
     2101 cagatagtcg ttgcttttcg catgttacct gaagcagttt ggaatatgtc cccaatgggt
     2161 accgttaatg tacacggaag cctgttacct caatatcgcg gcgctgctcc catcaactgg
     2221 gccataatca acggagaaaa agaaacaggg gtaaccactt tcaaactaaa acatgaaatt
     2281 gataccggag atatcttgtt acaggaacgt tttcctatcg gagacgatga aactgcagga
     2341 gaagtgcatg accgcatgaa agaaattgga gcaaagattt tagtggaaac ggtgaaaggc
     2401 ctggctgagg gcagtcttac tgaacaacct caaaccttag aactaaagcc tcaaacttta
     2461 gttcatgcgc caaaaattca tacggatacc tgcattattc attgggacag gccggttgaa
     2521 acagtccaca atctcatacg gggccttagc ccttatccgg gtgcatttac cattcttaat
     2581 gaaaaaatgc tcaaggttat ccgttctaaa aaagaaataa cctaccctaa aagtactgaa
     2641 ggtgattatg aaacagatgg aaggactttt ttaaagtttg cctgctcaga tggttatatt
     2701 cacttattag aagtgcagct cgaaggcaaa aagcgtatga gtattaccga ttttctgaaa
     2761 ggctaccgtt ttaatggcaa ttagtaattc actttgacaa taaacttatc ctgctcagta
     2821 atatcaagag cggctgcacc tgcattactg atacgtatat ccagtccctg gttttgacgg
     2881 ataccactca tttctcccaa aacctttgcg taaatagctt tattattgga aggattaatg
     2941 atgcggacaa tcgttccagg ttgaacattg tcaatgagta aataatattt cccatcctcc
     3001 cagccactga gtgttttaaa aatactggct gttacggttt catcttttga aggagcagac
     3061 aactttgatt gctgttcaaa atggttttta aaataccctt gcccttcaat ggtcactttt
     3121 cttacctctt ttacaacagg cggaggatct tttttctctt ctttcttctc agctttttct
     3181 tcatcttctg ccactttctt cacttccttt tttgtcaggt cagcttcttc cgttacatca
     3241 ggctgctttt ctttcttttc ctcagcaaca ggctggtcat ttttgggact gcctgaaaca
     3301 gtaatcgact tcatttcact gctcagtaaa aaaccaatga tcagctttgt atctttcttt
     3361 aactcatcag aagaaaggtt attccaccag cggaggttag ctaatgacac tttgttgtac
     3421 ttattactga cagacatcaa tccttctttc tcacccgttc tgtaataaac tggagttcct
     3481 ttaccgccat tttgtgtaaa attggtatca gtcaggggaa ttcttatttt ctgttcgatc
     3541 tgcagccctt tgctgatatc aagtttattg taagaagcaa tgaaccgggg atgaacattg
     3601 tataaccttc ctattgcaaa aaagctttct tttggagcaa ctttatgttc aagatacagg
     3661 cctttgtcgc ctttctttat catcaggtca cccttttgtg cggccagatg ccaggcgcat
     3721 aaaacaaatg acaaaacaaa taagaacaac ttcttcataa cgacaagatt tgggtgcaaa
     3781 gatgtgattt ttttgccgtt gatgacctta aaaatctttc aaaatccgca gttccttggg
     3841 gtaatagtta tttatcctgt taggtaagat attgacnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnng ctgaagatat tttatagcat cgtcataggt caactcgatt ttaccaaccg
     4021 aatcagctac taaaccactc atcgccaatg catgatccgg aatcaatttt tcccgcatca
     4081 gttcacctgc taaaacaccg gaataaacta ccctgagatg agcaagcagc agattaaaat
     4141 cattgtacag aatttctggc caggcataca cagtatcttc atgcttgatg aatgacaggc
     4201 catctgcctt cacccagtta ttgacaatag ccttttcctt aattgtaatt gattcaacct
     4261 tttctttgat tttaggagca acataaacat ctccctcctt tttttgaaag caggcaatgn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnact ttatctggcc aaaaccggta gcctccatca
     4381 gaaattgtaa ttccccaccc gggatcaacc gataaagcaa caggagccac tgaaaattct
     4441 ttattcagcc aatcaactat ttcttcgtct tcctgcctgg aataggaaca ggtagaataa
     4501 attaaaatgc catctttctt caaagcgggc aagacatcag ccagtatcct ttgctgacgc
     4561 tgtgaacata acgctacatt attctcactc cattcagcga tggcttccgg atctttacga
     4621 aataatccac taccactgca tggtgcatcc actataataa catcaaagta gttggacaac
     4681 ctggcaaatt ctttaggatc attattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcct
     4801 gtgtgattag tgactggata tgagtagatt tcccgccagg ggccgcacaa agatcaagca
     4861 ccctaaacgg tcttgacata tctgttgttt gccttaaagc ctgctccaaa aacatagacg
     4921 aggcttcctg tacataataa caaccagcat gaaacaaagg atcaaaggta aaggatgggc
     4981 gggattcgag ataatagcct tgctccgtcc agggtattcc ggacttttga ttttgaattg
     5041 ctgcttgcct taagggattc agccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta tctctgaaac
     5221 gaggtcttgc aaaacctttt tggcatcgcc aaataacatg gatgtttttg gccggaaaaa
     5281 aagatcattt tcaattcctg catacccggg cttcatgctg cgtttattaa caacaacatt
     5341 cttggcaagt tcaacatcga gaattggcat tccgtatatc ggggaagccg gatcagtttt
     5401 agcagcagga ttaacaacgt catttgcacc cagtataagt accacatcag ttgtagcaaa
     5461 ttcttcattg gcctgctcca tttccagcaa tttctcataa ggcacatcgg actcagcaag
     5521 taaaacgttc atgtggccgg gcatacggcc agccacgggg tgaatggcat aacgtacatt
     5581 aacccctttg ctctccagca atttttccag ttcatgacag gcatgctgtg cctgtgccac
     5641 tgctaatcca taaccgggga caatcatcac tttattggca taagccatga ccacagcagc
     5701 atcacttaat gtaatttcct ttacagcacc ttgttgcttt tgtccggcgg gtcctgcctt
     5761 actgcctcca aaagaaccaa tcaaaacatt tgttaatgac cggttcatcg ccttgcacat
     5821 cagtatggta agcaatgtac ctgccgcacc taccaggata ccgcctgtta gcatcgcctt
     5881 attattatat aaaaaacctc ctgttgcagc agctacaccg gtgaaggagt tcaacaaaga
     5941 aattacgacc ggcatatcgg ctccgccaat tggcataaca aacatgaccc catataaaaa
     6001 agcaaaaaag cccaacagca aaaatggtaa cattatattt ttaaactgtg actggtnnnn
     6061 nnnnnnaata cagctgcggc caatacaagt aacaggataa aaatatttac gagatgctga
     6121 cctttaaatg caaaatcctt gattctgccg tttaattttc cccatgctat cacactgccg
     6181 gcaaatgaaa cagatccaat aattgcccct gctattattg ttatatactc tcccaccggc
     6241 attacagatg aataaggaat atccattggc cccttgtaat gctggtacag atggttgaac
     6301 tctgcagcag aaatcaatgc cgcacatgca cctcccatac cattaaaaag actcaccatc
     6361 tcaggcatgg ccgtcatttt cactttcttg gctgccaatg tacctattac tgacccgata
     6421 atgatccctc caaagatcca ggcgtaattt ccgagttttt cacctccatc ctcatacaga
     6481 aaaattgtag caaatatggc aagagccatt cctgcggcag ccaccaggtt gccgtttctt
     6541 gccttggccg gattggaaag cattttcaga cctacaataa atgtaactga agcgatgata
     6601 taacaaagag taagtatatt aagttccata aagtcctttt actttttctt ctttttaaac
     6661 atttccagca tcctgtcagt aactacaaaa ccaccaacca catttagtgt tccgagtata
     6721 accgccagga agcctatgat aagggccagg tagttgtcac tttcggcttt acccattaca
     6781 atgatcgccc caattatgac aaccccatgt atggcattgg ctccactcat gagcggcgta
     6841 tgcaaaacac ttggcacatg accaatcact tctattccta caaaaatcat tagaatgact
     6901 atgtagatga tttgctggtt agccgtaatc cagtttagca atgaatccat atttttattt
     6961 taaacgttca tgtattattt cagcattatg agtaatacaa cagcctttca ccaggtcgtc
     7021 atcccagttc aggttaatag ctccttcttt cgtaataatc aactgtaaga aattaagcac
     7081 attttttcca tacatcttac ttgcatcaga gggcatggta gattgtaaga aggaattacc
     7141 aacaatactg actccattaa caacaactgt ctcattgttt ttagtaaacg gagtatttcc
     7201 acctgttgct gccgccaggt caattattac tgaaccattg cgcatagctc cgatcatttc
     7261 ttcagtaata agcaacgggg ccttttttcc gggaatttgc gcagtagtaa taataatatc
     7321 agcttttgct gcactttctg ctattttggc cttctgacgc tgcataaact cttctgactg
     7381 ttcaacagcg tatccgcccg ccttacttgc atcagctgcg ccttccactt ctataaattt
     7441 tgcaccaagg ctcattactt cttcctttac agcaggtctt gtatcaaaaa cctccactac
     7501 tgcaccaagc cttcttgcag ttgcaatagc ctgcagccct gcaacaccag cacctaagat
     7561 cagtactttg gcaggtgcta tactaccggc agctgtcatg aacatgggaa aatatcttcc
     7621 atatgtattg gcagcaagta aaacggcttt atacccggca atatttgcct gtgagctcaa
     7681 cacatccatg gcttgtgcac gggtggtgcg gggcagcata tccagggaaa acgtagtaac
     7741 ccctgcactt gcccattgtt tcattaactc aacgtgaaat aaaggttggt aaacgcctat
     7801 cagcaccttt gattggagat tagtgacttc actgagcccc ggctgatgaa tggacagtat
     7861 aatatcagaa gaagccaata cctcgttccg gctttttatt tgtgcccctg ctttcttata
     7921 ctccgtgtta ttacaaaatg ctttttcacc ggccccgtct tctataagaa catttattcc
     7981 tttttttgtc agcgtaccaa cagcttctgc caataaggat actcttgtct cgggggcagg
     8041 ttctttcaaa aggccaatcg tcataat
//
LOCUS       DAORLS010000130         8050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4837_length_8050_cov_1.671044, whole genome shotgun sequence.
ACCESSION   DAORLS010000130 DAORLS010000000
VERSION     DAORLS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4837_length_8050_cov_1.671044"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PK977_10690"
     CDS             complement(<1..574)
                     /locus_tag="PK977_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF18630.1"
                     /translation="MKNNNTVIDAHNLVRVYDEKTVPIYALNRVHLHIERGEFTAIKG
                     PSGSGKTTLLNMLGGLDSPTEGFVEINGTNITKLKGNKLIDFRLNNIGFVFQSYNLVP
                     VLTARENVEFIMLLQKRPTSEIKKRVNELLQQVGLSEKAGKRPSELSGGQQQRIAVAR
                     ALAPKPQFILADEPTANLDSVSAGNLLDIMA"
     gene            complement(571..>1388)
                     /locus_tag="PK977_10695"
     CDS             complement(571..>1388)
                     /locus_tag="PK977_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF18631.1"
                     /translation="RAGSYFNPQKENSILISEKLIKKLKLGIGKKTILTFQDSSGNMM
                     AMAYRITGVFKTSNTPFDENNVFVNISDIDSLAGLHHQYNEIAILLHSGSSLKVVQTK
                     LKSLFPEYEILNWQELAPEIALTVSVGEQMVLIFMGIILLALAFGIINTMLMAILERT
                     REIGMLLALGMNRLRIFQMVLWETVLLVLAGCPFGIGIALAAIGITHQTGIRFKKFSE
                     VYASFGYNDVIFPELNLRQFLIIMILVILTAVLSSLFPAWRALRLKPAVAMRK"
     assembly_gap    1389..1470
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1471..1783)
                     /locus_tag="PK977_10700"
     CDS             complement(<1471..1783)
                     /locus_tag="PK977_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18632.1"
                     /translation="MLLRIAWRNIGRNKKRSGIIIGAVTLGLAAGIFLMAFYSGMIEQ
                     RVRLAIQNETSHLQVHHPDFRKDYDIKYYLVNGPASQKQIQSQVEVKQAAGRIIIPGM
                     IA"
     assembly_gap    1953..1962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1963..>2503)
                     /locus_tag="PK977_10705"
     CDS             complement(<1963..>2503)
                     /locus_tag="PK977_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF18633.1"
                     /translation="QGYQGTLAAGKYRVKGIARFGSPDLNNRIIYLPLAATQAFLNAE
                     GLLTSLSFGIEQPEKMIPIKEQIDLMAGMSYEVMTWKELMPEIANHIRADGSSFYIFT
                     GILYLIIGFGIFGTILMMATERRYEFGMLIAIGMPKLRLGITLLIETIWITITGVLAG
                     VLISWPVVSWFSKNPIRISG"
     assembly_gap    2504..2544
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2545..3020)
                     /locus_tag="PK977_10710"
     CDS             complement(<2545..3020)
                     /locus_tag="PK977_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18634.1"
                     /translation="MILKLTWRNLWRNKRRTFITMASVTFAVVLAVAMQSFQRGIFDN
                     LVKNIVSFYYGYIQLHNKGYWDERIIDNGFISDDSLLLRLQKIKGVTKVAPRLETFVL
                     ASAGNTTKGAMLVGTDPKRENDFSQLQAKLVSGEYFNSNEESAILGEGLSERLNIKT"
     gene            complement(3017..3760)
                     /locus_tag="PK977_10715"
     CDS             complement(3017..3760)
                     /locus_tag="PK977_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRF18635.1"
                     /translation="MLPLRIIWLLSLIAPATFAQDATAIIRKADERAKGKSSFATFTI
                     QVIRPNWKREIKMKSWTKGNDLAMLLVTSPVKDKGIVYLKRKKEVWNWIPSIERTIKL
                     PPSMMSQSWMGTDFTNDDLVKEASIAEDYRHSIVGDSVIDERDSYKILLLPKPESAVV
                     WGKVLMWVDKKDNIIMYAEYYDEDGGMVNSLRTYDIKMLGGRLLPARMEMIPAAKKNN
                     KTVFIYNELQFDKPIDDNFFTSQNMPRLQ"
     gene            complement(3929..5908)
                     /gene="gyrB"
                     /locus_tag="PK977_10720"
     CDS             complement(3929..5908)
                     /gene="gyrB"
                     /locus_tag="PK977_10720"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRF18636.1"
                     /translation="MSTETTEIKPTVPGTYGADSIQVLEGLEAVRKRPAMYIGDIGVK
                     GLHHLVYEVVDNSIDEALAGYCKNITVNIHEDNSISVEDDGRGIPTAMHTKEKRSALE
                     VVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSTKLHVTVQREGKVFEQEYHIGVP
                     QYPVREIGSSDKTGTRVQFWPDGSIFITTTYNKDILEGRLRELAYLNRGVRITLNDLR
                     EKEEDGTNYSKSFYSEGGIVEFVEMLDQSAGRNSLIPKVIAVEGRDETTNVAVEVALS
                     YNDSYNEHIYSYVNNINTIEGGTHVSGFRRALTRVFKSYGDKNGLFEKAKVEIEGDDF
                     REGLSAIISVKVPEPQFEGQTKTKLGNNEVMGVVDSTVSKVLEAYLEENPKEAKNIVS
                     KVILAAQARAAARKARELVQRKSVLTGGGLPGKLADCSDRDPERCELFLVEGDSAGGT
                     AKQGRDRSFQAILPLRGKILNVEKAMEHKIYDNEEIRNMFTALGVKIGTPEDPKALDL
                     SKLRYHKLIIMTDADVDGSHIATLILTFVYRYMTELVEQGYVYIAQPPLYLVKKGKEQ
                     SYAWNEEQRKMWVEKLGAGKDESVTVQRYKGLGEMNAEQLWETTMNPQSRTLKQVTIQ
                     SAAEADRIFSMLMGDEVAPRREFIESHAKYAKLDV"
     gene            6059..>6319
                     /locus_tag="PK977_10725"
     CDS             6059..>6319
                     /locus_tag="PK977_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18637.1"
                     /translation="MKVRTCLLLLLLFVLSCRKNEENQPSPPPPVAYTAPKIDSSSFR
                     LGNLEKGTLDTFVLHYNRAVSVNFIRLLSAFCLPDMRWSVSAN"
     assembly_gap    6320..6334
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6335..>6563
                     /locus_tag="PK977_10730"
     CDS             <6335..>6563
                     /locus_tag="PK977_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18638.1"
                     /translation="SANGKTVKFYNLLCGRLAEDFTFEISVKDSTQQIKTDSVTFSYY
                     FKKIAVAAQMQQMYISPDNKFCWAILYDSAKL"
     assembly_gap    6564..6590
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6591..7457
                     /locus_tag="PK977_10735"
     CDS             <6591..7457
                     /locus_tag="PK977_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18639.1"
                     /translation="TSYRRTYNLPFRPWRFAFNLQNQKIYFVPYPFTGTEMNKVYVFN
                     PANGAIDKTITLIKDTYDDPLKRVLIYNIGFGSNGYGVLNTGTMEAGFSRWRIIDSRI
                     NDSVYAHPAWIASISGGNSNFREFASIQPNYNGTKIYMQAINAYPRAGLLDWVTQNLT
                     ELTLASGNPSHYLIPSKSHDKLFVASFSFQSILTSNTLWSNYSTFDNRYSETADFSYK
                     PGEQDVIYYRGRSGSYDFSVLDYANSRILTRINVRPDFHNINATTDGKYIIVSSGGGI
                     YFLKTAMIYQSF"
     gene            complement(7467..>8050)
                     /gene="fabZ"
                     /locus_tag="PK977_10740"
     CDS             complement(7467..>8050)
                     /gene="fabZ"
                     /locus_tag="PK977_10740"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRF18640.1"
                     /translation="LALIGYPIKGRIIANRPGHSTNVEFAKKIKQYIKKNKHTKGVPV
                     YDPNQPPVYDQQYIEKTLPHRFPFALVDKIIELSDTHIVGIKNVTFNEWFFQGHFPGN
                     PVMPGVLQIEALAQCGGILAINLSGGGQHDTYFLKIDNCKFKNMVRPGDTLILKMELS
                     APIRRGICEMKGTTYVGNKVCAEADLVAQIVKR"
BASE COUNT         2090 a         1762 c         1637 g         2386 t
ORIGIN      
        1 tggccataat atccagaaga ttacccgcag atactgaatc gaggttggca gtgggctcat
       61 ctgccaggat gaattgtggt ttaggagcca atgctcttgc cacagctatc cgttgttgct
      121 gacctccgga aagttcagag ggtcttttac ctgctttttc gctcagccct acctgctgca
      181 gaagttcatt cactcttttt tttatttcac ttgtgggtct cttttgcaat agcatgatga
      241 attcaacatt ttctcttgca gtgagcaccg gcaccaggtt gtacgattga aatacaaaac
      301 cgatattatt caggcggaaa tcaatgagtt tattgccttt tagttttgta atatttgtac
      361 cgttaatttc tacaaatcct tcggtggggc tatcaagtcc tcccagcata ttaagaagag
      421 tggttttgcc cgaaccagat ggccctttaa tagcagtaaa ttctcctctt tcgatatgca
      481 ggtgcacccg gttgagtgca taaatgggga ccgttttctc atcgtacacc cgtacgagat
      541 tatgcgcatc tatgacggtg ttattatttt tcattttcgc atggctactg ccggttttaa
      601 acgtaatgcc cgccaggcgg gaaataatga tgacaagaca gccgtgagta ttacaaggat
      661 catgatgatg agaaactgac gcaaattcag ttctgggaaa atgacgtcat tgtaaccaaa
      721 gcttgcatat acttctgaaa actttttaaa acgtatgccg gtttgatgag taatgccaat
      781 tgctgctaag gcaatgccaa ttccaaacgg acaacctgcc aacactagta atacggtttc
      841 ccacaacacc atttgaaaaa ttcttagccg gttcataccg agagccagta gcatgcctat
      901 ttcccttgta cgttcaagta tagccatgag catggtatta atgataccaa atgccagtgc
      961 aaggagaata atacccataa agatgagtac catctgttcg cctacagaaa ctgttagtgc
     1021 aatttcaggg gccagttcct gccagttcag gatttcatat tctgggaata ggctttttaa
     1081 cttcgtttgt acgactttta aagaagagcc tgaatggagc aaaatcgcta tttcattgta
     1141 ttgatgatga agtccggcca gggaatcaat atcagaaatg tttacaaaaa cgttattctc
     1201 gtcaaaagga gtgttgctgg ttttaaaaac accagtaatg cggtaagcca tagccatcat
     1261 attgccgctt gagtcctgga aggtcagaat ggtttttttg ccaatgccca gtttgagttt
     1321 tttaattagc ttttcactga tcagaatact attttctttt tgcgggttga aataagatcc
     1381 ggctctgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aagcgatcat tcccgggatg atgatacggc
     1501 cagcggcctg ctttacttca acctgtgatt gtatttgttt ttgactagca ggcccgttca
     1561 ccaggtaata tttgatgtca taatcttttc ggaaatcggg gtggtgcact tgaagatgtg
     1621 atgtttcgtt ttgaatagcc aggcgaaccc gttgttcaat catgccggaa taaaatgcca
     1681 tcaggaaaat accagcagca agtcccaggg taactgcacc gatgatgata ccgcttcttt
     1741 ttttatttct tcctatgttc cgccaggcga tccttagtaa catgttatta ttttttcatt
     1801 gccgttaccg ggtggagttt ataaacatac cataccgggt aaagtcccag caggaaggcg
     1861 atgattaata cagttaatga ctgcataatg aaaatgggca gatgtaccga agcagggaat
     1921 aatgcttcga agccgaatcg ttcatacaca tcnnnnnnnn nntgccgcta atacgaattg
     1981 gatttttact aaaccaggac acaacaggcc aactaatcaa tacaccggcc aatactcctg
     2041 ttatagtaat ccatatggtt tcgataagca gggtaatgcc gagcctgagt ttaggcatac
     2101 cgattgctat cagcatacca aattcatagc gtctttcagt agccatcatt agtatggtgc
     2161 caaaaatgcc aaaaccgata atgaggtata aaatgccggt gaaaatatag aagctgctgc
     2221 catcagccct gatatgattg gcaatttccg gcatcagttc tttccatgtc attacttcgt
     2281 atgacatacc tgccatcaga tcgatctgct ccttaatggg aatcattttt tctggttgtt
     2341 caataccaaa tgatagagag gtgagcaaac cttctgcatt gagaaatgcc tgggtggctg
     2401 ccagcggcag atagattatc ctattgttta ggtcaggaga accaaaacgg gcaatgcctt
     2461 ttacccggta tttgccagct gccaaagtac cctggtagcc ctgnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnngtttta atattcaatc tttcagataa accctcgcct
     2581 aaaatagcag actcttcgtt actgttgaag tattcaccgc taaccagctt agcctgcagt
     2641 tggctgaaat cattttccct tttcggatct gtgcctacca acatggctcc tttggtagta
     2701 ttaccagcag aggccagtac aaaggtttca aggcggggcg ctacttttgt tacacctttt
     2761 attttttgta acctgagcaa taaagaatca tccgaaatga aaccattgtc aataattcgt
     2821 tcatcccagt accctttatt atgtagttgg atatagccat agtaaaaact aactatattt
     2881 tttaccaggt tatcgaaaat tcccctttga aaagactgca tcgccactgc cagcaccaca
     2941 gcaaacgtaa cggaagccat agtgataaag gtgcgtcgtt tattacgcca taggtttcgc
     3001 caggtaagtt ttaaaatcat tgtagccggg gcatgttctg agaagtgaaa aaattatcgt
     3061 ctatcggttt gtcaaactga agttcattgt agataaatac tgtcttattg ttttttttgg
     3121 ctgccggtat catttccatt ctggcaggca ggagcctgcc tccaagcatt ttaatatcgt
     3181 aagtgcggag tgagttgacc atgccgccat cttcatcata gtattcagcg tacatgataa
     3241 tattgtcttt tttatctacc cacatcagaa ctttacccca taccacagcg gattctggtt
     3301 tgggcaagag caaaatctta taactatccc gttcgtcaat tacagaatct ccaactatac
     3361 tgtgccggta atcttctgca atagaagctt cttttaccag gtcatcgttt gtgaaatcgg
     3421 tgcccatcca gctttggctc atcatggaag gaggtaactt aatggtacgt tctatggaag
     3481 gtatccagtt ccatacttct ttttttcttt tcaggtaaac aatgcctttg tcttttacgg
     3541 gagaagtaac cagcagcatc gcaaggtcgt tacccttggt ccagcttttc atttttattt
     3601 ctcttttcca gttgggacga atgacctgaa tggtgaaagt ggcaaatgaa gatttccctt
     3661 tagccctttc atctgcctta cgaatgatgg cagtggcatc ctgtgcgaat gttgccgggg
     3721 ctatcaatga taacagccat atgatacgca gtggtaacat actgaactgt atgattacaa
     3781 agtaagctca ttacggcctg aaatcctttg atagtaatca taggctgata tgatcataag
     3841 gatggggaga aaaatagcgg ttcccaaaag caaaaaaccc cgaattggta ttggggtctt
     3901 ttgcttttga tcaatatctt gttactactt aaacatccag ctttgcatat ttagcatggc
     3961 tttcaataaa ctccctgcga ggggctacct cgtcacccat cagcatacta aaaatgcggt
     4021 ctgcctcagc tgcactctgg atggttacct gcttcaaggt ccggctctgg ggattcatcg
     4081 ttgtttccca taattgttca gcattcattt cacccagacc tttataccgc tgaacggtta
     4141 ctgactcatc tttaccggct cccagttttt ccacccacat tttacgctgt tcttcattcc
     4201 aggcatagga ttgctctttt cctttcttta ccaggtaaag tgggggctgg gcaatgtata
     4261 catagccttg ttctaccagt tcggtcatat aacggtaaac aaaagtcagg atcagcgtag
     4321 caatgtgaga accgtccaca tcggcatcgg tcatgatgat gagtttgtgg taacggagtt
     4381 tacttaagtc aagcgccttg ggatcttccg gagtaccaat ttttacgccg agggcggtaa
     4441 acatatttct gatctcttca ttgtcgtaga tcttatgctc catggctttt tcaacgttca
     4501 ggattttacc tcttaatggc aaaatggcct ggaaacttct gtcacggccc tgcttggcag
     4561 taccgccggc tgaatcaccc tcgaccagga atagttcaca acgctccggg tcacggtcgg
     4621 agcaatcagc aagtttgccc ggcaagccgc caccagtcag cacagattta cgctgtacga
     4681 gttctctggc ttttcttgca gcagcccttg cctgtgctgc aagtatcact ttggaaacaa
     4741 tattcttggc ttcctttgga ttttcttcta aataggcttc cagtactttg gatacggtag
     4801 aatcaaccac acccatcacc tcattgttgc ctagtttggt tttggtttga ccttcaaact
     4861 gaggttccgg cactttaaca gagatgatag cactgagccc ttcgcggaaa tcatcgcctt
     4921 ctatttctac tttggctttt tcaaacaagc cgtttttgtc accgtagctt ttgaacaccc
     4981 ttgttaatgc cctgcggaaa ccggaaacat gggtgccacc ttcgatagta ttaatgttgt
     5041 ttacataaga gtagatatgc tcattgtaac tgtcattgta gctcagtgcc acttccacag
     5101 ctacattggt agtttcatct cggccttcaa cggctattac ttttggtata agcgaattgc
     5161 gcccggcact ttggtccagc atttccacaa actccacgat accgccttca ctgtaaaaac
     5221 tcttgctgta gtttgtacca tcttcttctt tttccctcag gtcattaaga gttatacgga
     5281 caccccggtt caggtaggcc agttctctta ggcggccttc aagaatgtct ttgttgtaag
     5341 ttgtggtaat aaagattgaa ccatcgggcc agaactgaac ccgggttcct gttttgtcgc
     5401 tgctgcctat ttcccttaca gggtattgcg gaacgccaat atgatattcc tgttcaaata
     5461 ctttcccttc ccgctgaacg gtaacatgca gtttggtgct caatgcattc acacagctta
     5521 cacccacacc atgcaaacca cccgatactt tataggagcc tttgtcgaat ttaccccctg
     5581 catgcagtac ggtcattacc acttcaaggg cagaacgttt ttctttggta tgcatcgcag
     5641 tgggtatacc ccggccatca tcttcaacgg aaatagaatt gtcttcatgt atgttgacag
     5701 taatattctt gcaataaccg gccaatgctt catcaataga gttgtctacc acttcgtata
     5761 ccaggtgatg caaaccttta acaccaatgt cgccgatgta catggcagga cgtttgcgaa
     5821 cggcctctaa gccctctaat acctggatac tgtcagcccc atatgtgccg ggaactgttg
     5881 gttttatttc tgttgtttct gtgctcataa atggggtaaa atcctctggt taaaacggga
     5941 aaaaatataa ctcacaaatg tactgaaaca ggctggttag actagtattt tagcgctgtt
     6001 ttttgagaaa atttaccccg gttttagggt ttaaatagat tacttttaaa gataattttt
     6061 gaaagtgaga acctgcctgt tgttattgct attattcgtt ttaagctgcc ggaaaaatga
     6121 ggaaaaccag ccttcaccgc ctccgcctgt tgcctataca gcgcccaaga ttgatagcag
     6181 cagtttcaga ctgggaaacc tggaaaaagg caccttggat acttttgtgt tgcattataa
     6241 cagggcagtt tcggtgaatt ttatccgttt actttctgca ttttgcctgc ccgatatgcg
     6301 ttggtcagtt tctgctaacn nnnnnnnnnn nnnntctgct aacgggaaga cagtaaaatt
     6361 ttacaactta ttatgcggcc ggctcgccga agattttacg tttgaaatat cggtgaaaga
     6421 cagtacacag cagattaaaa cagacagtgt tactttctct tactacttca aaaaaattgc
     6481 cgtagcggca caaatgcaac aaatgtatat cagccccgat aataagtttt gctgggccat
     6541 cttatacgac tcggcgaaac tctnnnnnnn nnnnnnnnnn nnnnnnnnnn acctcctatc
     6601 gccgtacata caatctccct ttcaggccat ggcgatttgc attcaaccta caaaaccaaa
     6661 aaatttattt tgtgccatac ccgtttaccg gcaccgaaat gaacaaagtg tatgttttta
     6721 atcctgcgaa tggtgctatt gacaaaacaa taacattaat caaagacacc tatgatgacc
     6781 cgcttaaacg ggtattgatt tacaacattg gattcgggtc aaatgggtat ggcgtactca
     6841 atactggaac aatggaagcc ggtttttccc gttggagaat tatagattcc cgaataaatg
     6901 attctgtgta tgcccatccc gcatggattg cctccataag cggcggcaac agcaatttca
     6961 gggaatttgc atcgattcag cccaactata atggcaccaa aatatatatg caggccatca
     7021 acgcttatcc aagggcaggc ttgttggatt gggtgacaca aaatttgacg gaattaaccc
     7081 ttgcatccgg aaatcccagt cattatttaa taccaagtaa aagccatgat aaattgtttg
     7141 ttgccagctt ttcatttcaa agtattttga ccagtaatac tttgtggagc aactactcaa
     7201 cctttgacaa cagatattct gaaacggctg atttcagtta taagccaggt gagcaggatg
     7261 tgatttatta cagggggaga agtggttcct acgatttttc tgttcttgat tatgccaatt
     7321 cacgcatact tacaagaatc aatgtaagac ccgactttca caacattaac gcaacgaccg
     7381 acggaaaata cataatcgta tcttctggcg ggggcatcta ttttctgaaa accgccatga
     7441 tttatcaatc tttttagccc gtactactac cgcttcacta tttgtgctac caggtcagct
     7501 tctgcacaaa ctttgttacc tacataagtt gtgcctttca tctcacagat accgcggcgt
     7561 ataggtgctg aaagctccat tttcagaatc agtgtatcgc cggggcgtac catgtttttg
     7621 aatttgcaat tatcaatctt caggaagtag gtatcatgtt gtccgccgcc actcagattg
     7681 atggccaaaa tacctccgca ttgtgccagt gcttcaatct gtaatacacc gggcataacc
     7741 gggttgccgg ggaaatgccc ctggaaaaac cattcattga aggtcacatt tttgattcct
     7801 acgatatggg tatcgcttag ctcaataatc ttatctacca gtgcaaaagg aaaacgatgg
     7861 ggcagggttt tctcaatgta ctgctggtcg taaaccggcg gctggttggg gtcgtaaacc
     7921 ggaacacctt ttgtgtgctt gttctttttg atatattgtt tgatcttttt ggcaaactcc
     7981 acattggtgc tatgcccggg gcggttggct atgatgcgtc cttttatggg ataaccaatc
     8041 agtgccaggt
//
LOCUS       DAORLS010000131         8027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4860_length_8027_cov_2.074887, whole genome shotgun sequence.
ACCESSION   DAORLS010000131 DAORLS010000000
VERSION     DAORLS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4860_length_8027_cov_2.074887"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK977_10745"
     CDS             <1..541
                     /locus_tag="PK977_10745"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18641.1"
                     /translation="DMKALGSNNAEITVKGNGRLYYFWQSEGISSSGAYKEEDSYLKV
                     RKRFFDRTGKPITGITFKQNDLIIVGISLERSFNTAIENVVITDLLPAGFEIENPRTK
                     EIPGMDWIKDASTPTALDVRDDRIHFFVDAFNNKQTYYYAVRAVSPGNYKMGPVSADA
                     MYNGEYHSYNGAGVIRVVQ"
     assembly_gap    671..879
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <880..>1147
                     /locus_tag="PK977_10750"
     CDS             <880..>1147
                     /locus_tag="PK977_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18642.1"
                     /translation="YGSKTIEEQVEIAMYAEPRPIDAGRCNERYFINGVGIGFEGEVA
                     KALTGKKKLSGKTSFLLTILKKIFSYRSKHYEVKTESKNLEGKKL"
     assembly_gap    1148..1223
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1224..1458
                     /locus_tag="PK977_10755"
     CDS             <1224..1458
                     /locus_tag="PK977_10755"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18643.1"
                     /translation="GLLDVVIANALTPLQRLRYLPVIEKGKHLALPFINHFTSRNIKV
                     TSGAIIQYHLDGEYFEATQLDVEIMSKKFLFRY"
     assembly_gap    1528..1590
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1591..1918)
                     /locus_tag="PK977_10760"
     CDS             complement(<1591..1918)
                     /locus_tag="PK977_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRF18644.1"
                     /translation="MNIYVGNLSWNLKDQDLSNLFATHGAVTSAKIVTDKFTNRSKGF
                     GFVEMPNDDQAQAAIAALNGTEVDGRNIVVNESRPKPEGERGGGGGGFKKRSFGGGGG
                     GYKGGGG"
     gene            complement(2184..2630)
                     /gene="rplI"
                     /locus_tag="PK977_10765"
     CDS             complement(2184..2630)
                     /gene="rplI"
                     /locus_tag="PK977_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRF18645.1"
                     /translation="MEIILIQDVDNLGAKNEVVKVRNGYARNFLLPRQLAVENSPSNR
                     KQLEERLKQVRKKEEKMLAEINSVIAKLKEAPLKLGAKTGTSGKIFGSVTSLQISRAI
                     REQKGYEIDRKKISITDDIKELGTYKAVIDFGNGHNTEVEFEVVAE"
     gene            complement(2700..2963)
                     /gene="rpsR"
                     /locus_tag="PK977_10770"
     CDS             complement(2700..2963)
                     /gene="rpsR"
                     /locus_tag="PK977_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRF18646.1"
                     /translation="MAKNEIKYLTAIKSDKRVKKFCRFKKYGIRYIDYKDVEFLKKFL
                     NEQGKLLPRRLSGNSLKYQRKVSDAVKKARQMALLPYVTDLLK"
     gene            complement(2968..3324)
                     /gene="rpsF"
                     /locus_tag="PK977_10775"
     CDS             complement(2968..3324)
                     /gene="rpsF"
                     /locus_tag="PK977_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRF18647.1"
                     /translation="MNNYELMVIFTPVLSDDEFKAEQKKYAKLVTDAGGTVVAENPWG
                     LKSLAYPIQKKTTGLYWVMEYTAPTDFNEKLKIQMLRDENVLRHLCTKLDKYAVEYNA
                     KKRSGVKTGTEKAVEA"
     gene            complement(3564..4622)
                     /locus_tag="PK977_10780"
     CDS             complement(3564..4622)
                     /locus_tag="PK977_10780"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HRF18648.1"
                     /translation="MRKIIGILLFSLLSTSVLAQKKDYKAAVISFYNLENLYDTVDNT
                     MTNDEEFLPNGVRNYNSSIYFDKLGKLATVISQIGIEINPDGPAILGVAEVENDTVLN
                     DLIKQPLIAKRNYKIVHYDSRDIRGVDVGLLYNPKYFVVDASDKLFVQLPGGSKDAYF
                     TRDILWVKGKLGGETIHIYVNHWPSRSGGEQRSAPARNAAAQVCRNHMDSIAKLEPNA
                     KVIVMGDLNDDPTNESVEGIIKAKGKITDVRAGGMFNPWMDLYKKGIGTLAYQDAWGL
                     FDQILISHAWLNKDQDGFFFYQQHIFNKEYLVENKGRYKGYPMRTWDGNTYRGGYSDH
                     FPTYLVMLKEVKKEKKAF"
     assembly_gap    4858..4971
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4972..7364
                     /locus_tag="PK977_10785"
     CDS             <4972..7364
                     /locus_tag="PK977_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18649.1"
                     /translation="AGRDPFYSAASFNFSAVRFRIRGYDNDHFSTFMNGIPMDNLDNG
                     FTPFGLWGGLNDVMRNRDISWGLRYNTFTFGDIGGNTSIDSRASKQRAQTSVSYAASN
                     RNYNNRIMITHSTGMSKKGWAFSVSGSRRWADEGYVPGTYYNGWGYFLGVDKRIGQNH
                     LLSLTAFGAPTENGRQGPAVQEMMDLAGTNYYNPNWGYQNGKKRNASVGKTHQPIAIL
                     THDFRINNNTTLTTALGYSFGDRSVTALDWYNAADPRPDYYRYLPSYTSVWASTPDPV
                     QGQILYDLMKNDINTRQLNWQRLYDVNRASFATINDANGIAGNTVSGRRSRYIIEERI
                     INTTRFNANSVLNTKFGDHIDFTAGASYQQQNNHYYKKVDDLLGGEFYVNVNQFAERD
                     FPVDNVANQFDLDVPNRILKEGDKFGYDYNINISRIAAWAQGVFKFNKVDFFLAGTIS
                     QTEFYREGNKRNGLFPDNSKGKGIVNEFTNYAGKAGVTYKIDGRNYIFANGSYQTRAP
                     YFENVYVSPRTRNSQQDVVESETIQTVEAGYIMNAPKLKIRVGGYYTRFENAMDVMTF
                     YHDAYQNFVNYAINGIDKLHFGGEVGIEAKVTPTITLNAAAALGRYYFDSKQNAVITV
                     DNSASVLGKQVIYMENFKVPSTPMEAYSFGINYRSPKFWFVSLTGNYFDQSYLSMNPL
                     RRTWDALQYVAPKSDAYNEIFNQTKFDAQYTDFFGGYSWKLPKDFEVNKRPTFLVFNV
                     GVNNLLNNKEIITGGFEQLRYDAQASASDPVTVGKFPPKYFYAYGLNFFASMTLRF"
     gene            7416..>8027
                     /locus_tag="PK977_10790"
     CDS             7416..>8027
                     /locus_tag="PK977_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18650.1"
                     /translation="MKTLFLSFAASVLLFSCDKEPIGPTGPDPLGPGDYMSFTAFRAL
                     YPGSGEYLIPTGTKRIRGQVISNNTNEASGVYRLQDESGSGIYLYTVIGSPVYPMGTV
                     LDIDATGGGKLLLYNGDLELMSVPQANITQVSGTINITPRVATVAEIITNKNTWASSL
                     VKINNVTSISQASSNSTGVTYNVTDATGTLSMFVRNASGITVNT"
BASE COUNT         2165 a         1542 c         1579 g         2279 t
ORIGIN      
        1 tgacatgaaa gctttaggaa gtaacaatgc agaaataacg gtaaaaggaa acgggcggct
       61 gtattacttc tggcagagtg aaggtatcag cagcagcgga gcgtataaag aggaagacag
      121 ttacctgaaa gtgcggaagc gtttctttga ccgtacaggt aaacccatta ccggtattac
      181 ctttaaacaa aacgatttga tcattgtggg catcagcctt gaaagatcat ttaatactgc
      241 cattgaaaat gtggtgatca ctgacctttt accggctgga tttgaaattg agaacccaag
      301 aaccaaggaa ataccaggta tggactggat caaggatgct tccacaccta ctgcgttgga
      361 tgtaagagat gatcgcatac atttctttgt agatgctttt aacaacaagc aaacctatta
      421 ttatgcggta agggcagttt cacccggcaa ttataaaatg ggaccggtaa gtgctgatgc
      481 catgtataac ggcgagtatc attcatataa tggtgccggg gtaataagag tagtacagtg
      541 aaaataaaat gagtagtttt aaagccttcc gttatcggag ggctttttta tgcaagaagg
      601 tggccataga aatattgcaa ttgtgtgtaa tccgctggcg ggttcgggac gggcatcagg
      661 tattggcagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atatggtagt aaaacaatcg
      901 aagagcaagt tgagattgcc atgtatgctg agcccaggcc tattgatgcc ggccgatgca
      961 atgaacggta ctttatcaat ggagtgggca ttgggtttga aggtgaagtg gcaaaagcat
     1021 tgaccggaaa aaagaaacta tcgggaaaaa catcattctt gcttactatt ctgaagaaga
     1081 tttttagtta ccggtcaaaa cattatgaag taaaaacaga aagtaaaaac ctggaaggga
     1141 aaaagctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnntggctta ttggatgtag ttattgccaa tgcattaact
     1261 ccgctccaaa gattacgtta cttgcctgtt attgaaaaag gtaaacacct tgctttgcca
     1321 tttattaatc attttaccag tcgtaatatc aaggtcactt ctggtgcaat tattcagtat
     1381 caccttgatg gagaatattt tgaagctact caacttgatg tagaaataat gagcaaaaaa
     1441 tttttattca ggtactaaaa caaaaaagcc cgactctttc gaaccgagct tttaaagcga
     1501 gataaatatc gttattaatt aataatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgccgccgcc acctttgtag ccgccgccac
     1621 cgccgccgaa gcttcttttc ttgaagcccc cgccaccgcc gcctctttcg ccttctggct
     1681 taggacggct ttcgtttaca acgatgttac ggccatcaac ttcagtacca ttcaacgctg
     1741 caatagcagc ctgggcctgg tcgtcgttgg gcatttctac aaatccaaaa cctttgctgc
     1801 ggttagtgaa tttgtcggta acaattttag ctgaagttac tgcgccgtgt gttgcgaaaa
     1861 ggttggacaa atcctggtct ttcaggttcc aactaaggtt tcctacgtaa atgttcattt
     1921 tgagaggttt ttaaaaaata aaagtgtcaa cagtaaacag tacccaatga acattctacg
     1981 gtttttcgaa tcgttcagga cttgtgaaac tgaaagagca ccaaaataca gaacaaatta
     2041 acggattttt cccatacata ttgccaattt gtttaactgt aactggggtt aatcgttaat
     2101 ttattgattt tgagcaaata aaatattgaa caataaaaaa gtcctccatc agagaggact
     2161 tttatacgtt agattttaga cacttattca gcaaccactt caaattcaac ttcggtattg
     2221 tgaccgttac caaaatcgat tactgcttta taagttccca gttctttaat atcatcagtg
     2281 atgctgatct tcttgcggtc aatttcataa cctttttgtt cacggatagc acggctgatc
     2341 tgtaaagatg ttacgctgcc gaagatctta ccactggttc cggttttagc acccagtttc
     2401 aacggagctt ctttcagttt agcgattacg ctattgatct cagccagcat tttctcttct
     2461 ttcttacgaa cctgtttcag tctttcttcc agttgctttc tgttgctggg actgttttct
     2521 acagccagct gacggggtaa cagaaagtta cgggcataac cgtttcttac ttttaccacc
     2581 tcatttttgg caccgagatt atctacatcc tgaattaaaa tgatttccat atactttgtt
     2641 tttttcccaa tgaattacag gcttcattgt ctccgccggc tggcggatta gggacaggcc
     2701 tatttcaata aatctgttac gtaaggcagt aatgccatct gacgggcttt tttcactgca
     2761 tcagaaactt tgcgctgata tttcaggctg tttccgctga ggcggcgtgg caacaatttg
     2821 ccctgctcgt tcagaaattt tttcaggaac tccacatctt tataatcgat gtagcggatg
     2881 ccgtactttt tgaagcggca gaattttttt acccttttgt cgctttttat cgcggtgagg
     2941 tatttgattt cgttttttgc catggtatta agcctccact gctttttcgg ttcctgtttt
     3001 tacgccactt ctcttttttg cattgtactc gacggcgtat ttatcgagtt tggtgcacag
     3061 atgacgaagt acattctcgt cacgaagcat ttgaattttt agcttctcgt tgaagtcggt
     3121 tggcgcagta tattccatta cccagtataa accagtggtt tttttctgga tggggtacgc
     3181 cagtgatttc aatccccagg ggttttcagc tacaacagtt ccacctgcat cagtaaccaa
     3241 tttggcatac ttcttttgct cggctttgaa ctcgtcatcg cttaaaacag gggtaaaaat
     3301 caccatcaat tcgtaattgt tcatttacct gttttttatc cgccagctgg cggaatggtt
     3361 taaaaaaatt attatcccat tgggcaaccc cgaaaacaat cagggaagaa tcccgcaacc
     3421 atgcaggatt tggggcggca aagatagggg aatgtggcca caagtagcgg tctggatata
     3481 ggaaaattta gcttagccct ccataaagaa tcgcaatagg gttaaattaa caatctgagt
     3541 tgtaaggcat tgaaaagcag tgcctaaaac gctttctttt ccttctttac ctcttttagc
     3601 atcaccaggt aagtagggaa atggtcgctg taaccgcccc ggtaagtatt gccatcccag
     3661 gtacgcatcg ggtatccttt atagcgacct ttattttcta ccaggtattc cttattaaaa
     3721 atgtgttgct gataaaagaa aaacccatcc tggtctttgt tcagccatgc atgagagatc
     3781 agtatctggt caaataaacc ccaggcatcc tggtaagcca atgtgccaat gcccttttta
     3841 tataaatcca tccatgggtt gaacattccg cccgccctta catctgtgat ttttcctttg
     3901 gcttttatta tgccttccac actttcgtta gtgggatcat cattcaggtc gcccataaca
     3961 ataacctttg cattgggttc cagctttgcg atggaatcca tatgatttct gcaaacctga
     4021 gctgctgcat tccttgccgg tgcactgcgc tgttcgcctc cgcttcggct gggccagtga
     4081 tttacataaa tatgtattgt ttcacctccc aattttcctt tcacccataa gatatcacgg
     4141 gtaaaatagg catccttaga gccacccgga agttgaacga ataacttatc gctggcatcc
     4201 accacaaaat attttggatt atacaagaga cctacgtcta cgcctcttat atctcttgag
     4261 tcgtagtgaa cgattttata atttctttta gcaataagtg gttgctttat cagatcattt
     4321 aataccgtat cattttctac ttcggctaca cccaatattg ccggcccatc ggggtttatt
     4381 tcaattccaa tttgagaaat gacagtagcc agtttgccca gtttgtcaaa atagatggaa
     4441 gagttatagt ttctaactcc gtttggaaga aattcctcgt cattggtcat ggtattatcc
     4501 actgtatcgt agaggttctc cagattataa aaagatataa cggcggcttt ataatccttt
     4561 ttctgtgcta agactgaagt acttaacaat gaaaaaagta aaattcctat gatctttctc
     4621 atgttgaaaa aataataagg cgcaaactta gtgaaacaat agacgaatgc aacaaataaa
     4681 aggatgacat cagttaataa aatattatgt gtttatcttt ttttcggtct gtacttgtat
     4741 catcagtaat tttgcactct tgtttaaaaa ttaaatcatg cttaaaacca gactgttact
     4801 gctgctctta tgcgccggtc tttttacttc ggcgcaggtt gattccacag caaagaannn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggcgggaa
     4981 gagatccttt ttactcagca gcttctttta atttcagcgc tgtacgattc cgtatccgtg
     5041 gatacgacaa tgaccatttc agcactttta tgaatggcat tcccatggat aaccttgata
     5101 atggatttac gccatttggg ttgtggggcg gcttaaatga tgtaatgcgc aacagggata
     5161 tatcctgggg tcttcgctac aatactttca catttggcga tattggaggg aatacaagta
     5221 tcgactcccg tgcaagtaaa caaagggcac aaacaagtgt cagttatgca gcctccaatc
     5281 gtaactacaa taacaggatc atgattactc attccaccgg aatgagcaaa aagggatggg
     5341 ctttctctgt aagtggcagc cgccgctggg cagatgaagg atatgtgcca ggtacctatt
     5401 ataacggatg gggttatttt ctcggtgttg ataaaagaat aggccaaaat cacctgcttt
     5461 ctttaactgc atttggtgcc ccaacggaaa atggccgcca gggtcctgca gttcaggaaa
     5521 tgatggacct tgcaggtact aattattata atcccaactg gggttaccag aatggtaaaa
     5581 aaagaaatgc cagtgtgggc aaaacgcatc agcctattgc tatactgaca catgattttc
     5641 gaatcaataa taacactacc ctgacaacag cgcttggtta ttcttttggt gacagaagtg
     5701 ttacagctct tgactggtac aatgcagccg acccaaggcc tgattattac cgatacctgc
     5761 caagctatac atccgtttgg gccagcacac ctgatccggt acaggggcag attttatatg
     5821 atttgatgaa aaacgacatc aatacccgac agcttaactg gcagcggtta tatgatgtaa
     5881 acagggccag ttttgcaacc atcaatgatg caaatggaat tgcaggtaat actgtcagtg
     5941 gtcgccgttc caggtatatc attgaagaaa gaatcattaa tactacccgc tttaacgcca
     6001 actcagtact gaatacaaag tttggtgatc atattgactt tacagccggc gcttcttacc
     6061 agcagcaaaa caatcactat tataaaaaag tagatgactt attgggcgga gaattttatg
     6121 tgaatgtgaa ccagtttgct gaaagggatt ttcccgttga caatgttgcc aaccagtttg
     6181 acctggatgt gcctaaccgg attttgaaag aaggagataa gttcggttac gattataata
     6241 tcaatatctc aagaatagct gcatgggcac aaggcgtttt taaattcaac aaagtagatt
     6301 tcttcctggc cggtactatt tctcaaacgg aattctaccg ggagggaaat aagcgtaatg
     6361 gtcttttccc ggataattca aaaggtaaag gaattgtgaa tgagtttacc aattatgccg
     6421 gtaaagcagg ggttacttat aaaatcgatg gaaggaatta catttttgcc aatggctcct
     6481 accagacaag ggcaccttat ttcgaaaatg tgtacgtatc gccccgtacc cgcaacagcc
     6541 agcaggatgt tgttgaaagt gaaactatac agactgttga agccggctac atcatgaatg
     6601 caccaaaact gaaaatcagg gtgggtggtt actatacccg ttttgaaaac gcaatggatg
     6661 taatgacttt ctatcatgat gcttaccaga attttgtgaa ctatgctatc aatggtattg
     6721 ataaacttca ttttggtggt gaagttggta ttgaagcaaa agttactccg actattacgc
     6781 ttaatgctgc cgcagcatta ggccgttact attttgacag caagcagaat gcagtcataa
     6841 cagtagataa cagcgcatct gtattaggaa agcaggtgat ttatatggaa aatttcaagg
     6901 tgccatcaac accaatggaa gcttatagtt ttggtattaa ctatcgttca cccaagttct
     6961 ggtttgtaag tcttaccgga aactattttg accagtcata ccttagtatg aatcccctgc
     7021 gccgcacctg ggatgcttta caatacgtag ctccaaaaag tgatgcctac aacgaaatat
     7081 tcaaccagac aaagtttgat gcgcaatata cggacttctt tggcggctat tcctggaaat
     7141 tgccaaaaga tttcgaggtt aacaaaagac caactttcct ggtatttaat gtaggtgtaa
     7201 ataacctttt gaataacaaa gaaattatta ccggcggttt tgagcaactt cgttatgatg
     7261 cacaggcatc tgcttctgat cctgtaactg ttggcaaatt tcctccaaag tacttttatg
     7321 catatggcct gaactttttt gccagcatga cacttcggtt ttaatcaatg aataaaagtg
     7381 aaaaatttat gcgactaaat caattaaata aaattatgaa aacactgttt ctctcatttg
     7441 cggcaagtgt tttactgttc tcttgcgata aggagcctat aggcccgaca ggcccggatc
     7501 cacttggccc cggtgattat atgagtttta cagctttcag ggcactatat cccggcagcg
     7561 gtgagtatct gattcctact ggtaccaaac gtatcagggg acaggttatt tccaataata
     7621 ccaatgaagc ttctggtgtt tatcgactgc aggatgaaag tggttctggt atttatcttt
     7681 atacagtaat tggttcaccg gtttacccaa tggggactgt gcttgatatt gatgctacag
     7741 gtggtggtaa acttttgttg tataacggtg acctggaact gatgagtgtg ccacaagcta
     7801 atattacaca ggtaagtggt accattaaca ttactccaag ggttgccaca gttgcagaga
     7861 taattacgaa taaaaatacc tgggcttcat cgttggtcaa gatcaataat gttacatcta
     7921 tctcgcaggc atcttccaac tcaaccggtg tgacttataa cgttactgat gccaccggta
     7981 ccctgtccat gtttgtaaga aatgcatccg gcattacggt aaataca
//
LOCUS       DAORLS010000132         7998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4888_length_7998_cov_2.034244, whole genome shotgun sequence.
ACCESSION   DAORLS010000132 DAORLS010000000
VERSION     DAORLS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4888_length_7998_cov_2.034244"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK977_10795"
     CDS             complement(<1..258)
                     /locus_tag="PK977_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18651.1"
                     /translation="MLKRFTILLLLVVITASANAQNMRFSVATDLGGQRSFKKGQQFW
                     ALGHTVHALFNLTPKDGIYVWFAYYTNGKFTNELTAAAKSPL"
     gene            complement(360..>2440)
                     /locus_tag="PK977_10800"
     CDS             complement(360..>2440)
                     /locus_tag="PK977_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF18652.1"
                     /translation="QVYNDMVKKGLKLTKEQLYNVNKASLKDAIIIFGGGCTGEIVSA
                     EGLIFTNHHCGYDAIASASTMDHNYLKDGFYAKNKAEEIRTNLSVQFLLRIDDVTKEV
                     MDSLKGLSGAERVQKQNAVLAAINKRMSDADKNIETRVSALFKGNQFLAFVYQRYFDV
                     RLVGTPPESVGKFGGDTDNWEWPRHTGDFSVFRVYTKPDGSPAKRDEATEGNIPMKPK
                     WYLPVSLKPLKDGDFSMIWGYPGGTNRYESSYGIKLATDINNPTLVSLRDVRLKYMFE
                     EMKKDPAIKLQLASSYAGIANYWKFFDGETKQLLKYDIYGQKKAAEEKFNAWAKGKPE
                     YENLFADWGKAYDAWRPYAKVRMYMNEGIFGSPLIGFAASLMQLENLLLKPGTPQADI
                     KKAMDAATNQRKGFLEDENKASDQNIVAAVTRMFYENVDKEQHPIGFYGAVKDKYGPL
                     NDDATYKKYAADLFSNTMIFDDAKWAAFAANPDALKLQDDPAFSHASAFLKNYNSKYA
                     PYFQQFNAKNAEFGRLYLKGIMEMDPVKAKMMYPDATFTMRVSYGNVKSYRPRDAVFY
                     DYVTTSKGLLEKYKAGDYEFDLPAKQIELLKKKDFGQYMDPTRKDLVIGFITTNDITG
                     GNSGSPVLNNKGELIGLAFDGNYEALSHKLAFDKDLNRTICVDIRYVLWCIDKLGGGS
                     NIIKELKLMK"
     assembly_gap    2441..2450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2792..2878
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2879..4007
                     /locus_tag="PK977_10805"
     CDS             <2879..4007
                     /locus_tag="PK977_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HRF18653.1"
                     /translation="SPSTIIASNVTATSYSYSVPVTATSTTYYWYVQPKNATGGATGC
                     ATTATSFTYTVIQTPPAFGYYVVGYLPSYRSVTSIPDVKFRMTNVVVYAFYAVNTAGT
                     LSAPSSPASTLVAVRDKARANNAKVLLGINDGSGDGKTNFKNMASTAAGRNNFIKDVM
                     NVVRANNLDGVDMDWEFPSTSDGTDATFTLLMKELSDSLHRDGRYYLSAAITAGKYAG
                     GIRDAIRAEIFPYVDFFNIMAYDDFSTTVPYRHHSDYTLATVCLNYWLNTRGMPASKA
                     VLGIPAYGRPSGITQSNTILTYNGILGQGGNPQYDSAVVTASGYPSPYTIYYNGQYTT
                     KRKAKLAKDIANGVMFWEKGQDAHDVNSLMKAACDTVGRAY"
     gene            complement(4254..5255)
                     /gene="obgE"
                     /locus_tag="PK977_10810"
     CDS             complement(4254..5255)
                     /gene="obgE"
                     /locus_tag="PK977_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRF18654.1"
                     /translation="MEKSNFVDQIRIFCRSGHGGAGNKHFMRNKFTALGGPDGGDGGR
                     GAHIILKGNRNLWTLLHLRYYKNVLAEDGEKGGANNCHGRDGKDIIIEVPLGTVARDE
                     ETGIQEVEILEDGQEVIWLPGGKGGLGNSHFATPTNQAPEHAQPGLPGIEGWKILELK
                     VLADVGLVGFPNAGKSTLLSVITAAKPKIADYAFTTITPNLGIVEYRDGKSFCIADLP
                     GIIEGAAEGKGLGHRFLRHIERNSVLLFLIPADSNDHKKEFDILVNELEQYNPELLHK
                     QFIIAISKSDMLDDELKEEIKKQLPDNIPHLFISSVTNKGLTELKDLLWKVLNQPVE"
     gene            5315..5695
                     /locus_tag="PK977_10815"
     CDS             5315..5695
                     /locus_tag="PK977_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18655.1"
                     /translation="MKYLLLMFAIPFLSASDCGSKKDKEAAAGNSDTMALPACVQKLI
                     DDAKKEEPPMPPVQVDEYVYKGKKVYLFTAQCCDFYNMVYDDSCKTLCAPSGGFTGKG
                     DGKCPDFDSTAKLVRTIWKNPANK"
     gene            complement(5764..7023)
                     /locus_tag="PK977_10820"
     CDS             complement(5764..7023)
                     /locus_tag="PK977_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18656.1"
                     /translation="MRKCLPFFSLFSGALLLTSTLTAQQADRFAYAVTDIQQGGANWS
                     FLRKINLQNGEYSQVLLSGNDATLLAYDATTRKQFEAPLQDSRFGNYVNAAFATGVAA
                     MAYDKKNNRLYYTPMFIDQLRYIDLKTMKVYYVTDQAFTGKPVKSSDQGNIVTRMVIA
                     SDGYGYAMTNDATQLIRFTVGKKLSITDLGTIADDQANKGVSIHNSCSSYGGDIIADD
                     AGNLYVFSARNQVFKINLETKVATHLGTISGLPNGFTVNGAAVTNDNKVLVSSAMLAS
                     SYFTVDMKSLSATPYTIAGTVWQSSDLANGNLLNTGTQAKGNTVELISRNTEPNSGDG
                     KISIFPNPVTNNQFAIQFNELEAGQYTIQVTDVMGRQVLQQIVSVAGEKQIQTVRLNA
                     SSSKGVYLVKVTDHANRSVYNTKIVVQ"
     gene            complement(7116..7700)
                     /locus_tag="PK977_10825"
     CDS             complement(7116..7700)
                     /locus_tag="PK977_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HRF18657.1"
                     /translation="MIYRFKEYIPVIDESSFVHPQAAVTGNVIIGKNCYIGPGAALRG
                     DWGQIILEDGCNVQENCTIHMFPGVTVVLKEGAHIGHGAIIHGAAIGRNCLVGMNAVV
                     MDNAELGDECIVGALCFIKQDEKIPRRSLVAGNPGKIIKEVSDEMIAWKTEGTKLYQQ
                     LPAECFQYLEPAEPLTVVPADRQVQTGMYKPFKK"
     gene            complement(7728..>7998)
                     /locus_tag="PK977_10830"
     CDS             complement(7728..>7998)
                     /locus_tag="PK977_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRF18658.1"
                     /translation="IHGGIAFSLADSAFAFACNNRNVLSVALDTSINFIKPVHVGDVL
                     TAEAKEMHNGKSTGLYHIAIFNQHDHVVAQFKGLCYRTDKKLISV"
BASE COUNT         2079 a         1744 c         1755 g         2323 t
ORIGIN      
        1 gagtggtgat ttggcagctg ctgtcagttc gttggtaaac tttccgttgg tgtagtaagc
       61 aaaccagaca tagatgccgt ctttcggtgt aaggttgaac agggcatgta ccgtgtggcc
      121 gagtgcccag aactgctgtc cctttttgaa actgcgttgc ccgcccaggt ctgtggccac
      181 actgaaccgc atgttttgtg cattagcgga tgcagtgata acaaccagca gcaggagtat
      241 ggtaaatcgt ttcagcaaga aggataacgt tgttgcaatg atactgaaat aattgcttgc
      301 ttaaaacaga aaagccaccc tgtattacag gatggctttt acagaagagg agatgataat
      361 tatttcatca gcttcagctc tttgatgata ttgctgccgc cgcctaattt gtcgatacac
      421 cacaatacgt agcgaatatc cacacagata gtacggttga ggtctttatc aaaagccagc
      481 ttgtggctca gcgcttcgta gttgccatcg aaagcaagac cgatgagttc acctttattg
      541 ttcagaacag gtgaaccgga attaccaccg gtgatatcat tggtagttat gaaaccgata
      601 accaggtctt tacgggtggg gtccatatac tggccgaaat cttttttctt cagcaattcg
      661 atctgctttg caggcaggtc aaattcataa tcacctgctt tgtatttttc cagcaaacct
      721 tttgatgtgg taacatagtc gtagaacaca gcatctctcg gcctgtaaga ttttacatta
      781 ccatagctca ctctcatggt gaacgtagca tcaggataca tcattttcgc ttttacggga
      841 tccatttcca tgatgccttt caggtacagc ctgccaaact cagcattctt cgcattgaac
      901 tgctggaagt aaggagcata cttgctgtta taattcttta agaaagcaga tgcatggctg
      961 aatgcagggt catcctgcag tttcagcgca tcaggattcg cagcaaaagc agcccatttg
     1021 gcatcgtcga atatcatagt gttgctgaac aggtcagctg catatttctt ataagtagca
     1081 tcatcattca gcgggccata tttatctttt acggcaccat agaaaccaat cggatgttgt
     1141 tctttgtcca cattttcgta gaacatacgg gtaacagcag ccacaatgtt ctggtcagat
     1201 gctttgttct catcttcgag gaaacctttg cgctggttag tggcagcatc cattgctttt
     1261 ttgatatctg cctgtggggt gcctggtttt aataacaaat tttctagttg catcagcgaa
     1321 gcggcaaaac caatcagtgg tgatccgaaa attccttcat tcatgtacat tctcactttg
     1381 gcataaggtc tccatgcatc ataggcctta ccccagtcag caaagaggtt ttcgtattcg
     1441 ggtttgcctt ttgcccaggc attgaatttt tcttctgcgg ctttcttttg accgtatata
     1501 tcatacttaa ggagctgttt ggtttcgcca tcaaagaatt tccagtagtt ggcgataccg
     1561 gcataagaag aagccagctg cagtttgata gccgggtctt tcttcatttc ttcaaacata
     1621 tatttcaggc gaacatctct caatgatacc agtgttggat tgttaatatc agtagccagt
     1681 ttgataccat aagaactttc ataccggttg gtaccgccgg gataacccca gatcatagag
     1741 aagtcaccgt ctttcagcgg ctttaatgaa acgggcaggt accatttggg tttcatcgga
     1801 atgttgcctt cagtagcttc atctcttttg gcagggcttc cgtcaggttt tgtgtacaca
     1861 cggaatacag agaagtcgcc ggtgtggcgt ggccattccc agttgtcagt gtcgccgcca
     1921 aacttaccta cgctttcagg aggagttcct acaaggcgaa catcaaaata acgctggtat
     1981 acgaaagcaa ggaactggtt gcctttgaac agggcactta cccttgtttc aatgttttta
     2041 tctgcatcac tcattctttt attgatagca gcgagtacag cattttgttt ttgtaccctt
     2101 tcagcaccgc taaggccttt cagagagtcc atcacttctt tggtcacatc gtcaatacgt
     2161 aacagaaatt gtactgacag gttagttctg atctcttcag ctttgttttt tgcataaaaa
     2221 ccatctttca ggtagttatg atccatggtg ctggcagaag caatggcatc gtaaccacag
     2281 tggtggttgg taaagatcag gccttcggcg cttacgatct caccggtaca acctccgcca
     2341 aagatgatga tcgcatcttt cagcgaggct ttattgacat tatacaattg ttctttggtg
     2401 agctttaatc cttttttcac catgtcgtta tacacctgct nnnnnnnnnn cagcagcagt
     2461 ggcagccaca tgccttcgtc ggcaaatgac cgtacggcaa ggctgataaa taatacaatc
     2521 gttaacagga acttcttcat actagaaatt ttagggggca aacctacgta aaaacagcca
     2581 gcgaagtgct gagtttcgtc cggatttact attttgaaga cccaatcatt tgcttatgag
     2641 attcaatcgc ttgtcctttg ttttgttggt attttctgtc gtactgatcc attcctgtag
     2701 taagaaaagc ggaggagggg gcggtaaccc gccgccaccg aatgttccag gctgtgcttc
     2761 caacacggct cctgcaaacg gaactatgct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     2881 gcccctccac catcattgcc agcaacgtta ctgcgacgag ttatagttat tctgttcctg
     2941 ttacagctac aagtactacc tattactggt atgtgcagcc aaagaatgcc actggcggtg
     3001 ctaccggctg tgctacaacg gcaacatcat ttacctatac agtaatacag acaccaccgg
     3061 cttttggtta ctatgtggta ggctacctgc cttcctacag aagtgtgacc agtataccag
     3121 atgtaaaatt cagaatgacg aatgtggtgg tgtatgcctt ttatgcagtt aatactgccg
     3181 gcactttgtc tgcaccttcc tcaccggctt ctacattagt ggcggttaga gataaggcaa
     3241 gggccaataa tgccaaagtg cttttaggga tcaatgatgg cagcggcgat ggcaaaacga
     3301 attttaaaaa catggcgtct actgcagccg gtcgcaataa ttttattaaa gatgtaatga
     3361 atgtggtaag ggccaataac ctggatggtg tggatatgga ctgggaattt cccagcacat
     3421 ccgacggaac agacgccaca tttaccctgc tgatgaaaga actaagtgat tctttgcaca
     3481 gggatggccg ttattattta tcagcagcta ttacggctgg taaatatgcg ggtggaatcc
     3541 gtgatgcaat ccgtgccgag attttcccct atgttgattt cttcaatatc atggcttatg
     3601 atgattttag cacaacggtt ccttaccgtc atcatagtga ttatacattg gctacagttt
     3661 gcctgaacta ttggctgaat acccgtggca tgcctgcctc gaaagccgta ttaggtattc
     3721 cggcttatgg aaggccttca gggattacgc aaagcaatac aattctaaca tacaatggta
     3781 ttttaggaca gggtggtaat ccgcagtatg attctgctgt tgtaactgca tcagggtatc
     3841 cttcacctta taccatctat tacaatggtc agtacacaac aaagcgtaaa gccaaactgg
     3901 caaaagacat cgctaatggt gtgatgtttt gggaaaaagg ccaggatgcc catgatgtca
     3961 actcgttaat gaaggcagcc tgtgatacgg tgggaagagc ttattaattg cagatttata
     4021 tttcagattt tagattgaca tctttgccca gatctggaag ttcttttgtt tgccgggagt
     4081 gcttggttgt atttatatgt tgtatcacgg aggtcagtaa aaggttggtg tcacagttat
     4141 ctccatcaca acagggaagg gcattggtgt ggcatacggg acattgataa tgtccatgta
     4201 catgaacgag tgatacaata tggccgcaga agtgacagcg ctggagttgc atattattct
     4261 acgggttgat taagtacttt ccacagcagg tctttcagtt ctgtcaatcc tttattggtg
     4321 acagaggaaa tgaaaagatg cggaatatta tcgggtaatt gttttttgat ctcttctttc
     4381 aattcatcat ccagcatatc gcttttgctg atagcgataa tgaattgttt atgcaaaagt
     4441 tccgggttgt attgctccag ttcatttacc aatatatcaa attccttttt atggtcatta
     4501 ctgtctgcag gtatcaggaa taacaacacg gaatttcttt cgatgtgccg gagaaacctg
     4561 tgccccagtc cctttccttc agcagcgcct tcaatgatac cgggcagatc ggctatacaa
     4621 aaacttttac catccctgta ttctacaata ccgagattgg gggtgatggt ggtgaatgca
     4681 tagtcggcaa ttttgggttt ggccgcagta atcactgata acaatgttga tttacctgca
     4741 ttgggaaaac ccactaaacc cacatcggcc agcactttta gttccagaat tttccatcct
     4801 tctataccgg gcaggccggg ttgagcatgt tcaggcgcct ggttggtagg tgtggcaaaa
     4861 tgagaattac ccaaaccgcc tttacctccc ggcagccaga tcacttcctg gccgtcttcc
     4921 aaaatctcga cttcctgaat tccggtttcc tcgtctcttg caacagtgcc caagggtact
     4981 tctatgataa tatcctttcc gtccctgcca tggcaattat tggcgccacc tttttcacca
     5041 tcttcagcca gtacattttt ataataccgt aaatgaagca atgtccagag attcctgttt
     5101 cctttcagaa taatatgagc cccgcggcca ccatcgccgc catcgggtcc gcccaatgct
     5161 gtaaacttat tgcgcatgaa atgtttgttg ccagccccgc catgcccgct gcgacaaaat
     5221 atccgtatct ggtcaacaaa attgcttttt tccaaaaccg tatttttatg atgtgctgca
     5281 aaaatatact ttagtggtca ctaacaacca acttatgaag tacttgcttt taatgtttgc
     5341 gataccgttt ttatcggcca gtgactgtgg cagtaaaaag gataaagaag ccgctgccgg
     5401 taacagtgat acaatggcct tgccggcctg tgtgcaaaag ctgatagatg atgccaaaaa
     5461 agaagaacca cccatgccac ctgtacaggt agatgaatat gtttataaag gcaaaaaggt
     5521 atacctgttt accgcccagt gctgcgattt ttataatatg gtatatgatg acagctgtaa
     5581 aaccttgtgt gcaccatcag gcggtttcac cgggaagggt gatggcaaat gtcccgactt
     5641 tgattctact gcgaaactgg taaggacaat ctggaagaat ccagctaata aataattgat
     5701 aaagtattta aacgaaaaat gccccgtcag agccgacagg gcatttttac ttcagggaca
     5761 ttgttattga accacaatct tagtattgta aactgacctg ttggcatgat cagttacttt
     5821 aaccaaatat actccttttg atgaagaagc attcagtctt actgtttgta tttgtttttc
     5881 tcctgctaca cttacgatct gctgcaacac ctgcctgccc attacatcag taacctgtat
     5941 ggtatactga cctgcttcca gttcattgaa ctgaatagca aactggttat tggtaacagg
     6001 gttggggaaa atggaaattt tgccatctcc tgaattaggt tctgtattgc ggctgatgag
     6061 ttccactgta ttgcctttgg cctgggtgcc ggtgttcaac aggttgccat tggccaggtc
     6121 tgaactttgc caaacggttc ctgcaatggt ataaggcgta gcagacagcg atttcatatc
     6181 cactgtaaaa taggaactcg ccagcatggc gctgcttact aatactttgt tgtcattggt
     6241 aacggcagca ccgttcacgg taaaaccatt gggtaaaccg gaaatagtgc caagatgggt
     6301 ggccactttt gtttccaggt tgattttgaa tacctggttg cgggcagaga atacataaag
     6361 gttgccagca tcatcagcaa taatgtcgcc accatagctg ctgcagctgt tatggataga
     6421 tactccttta ttggcctggt cgtcagctat agtgcccaaa tcggtgatgc tgagtttttt
     6481 tcccacagtg aaacgaatca gttgagtggc atcattggtc atggcataac catagccatc
     6541 agaagcgatc accattcttg ttacgatatt gccctggtca gatgatttta ccggtttgcc
     6601 tgtaaaagcc tggtcagtta cgtaatacac tttcattgtc ttcaggtcaa tgtagcgtaa
     6661 ctggtcaatg aacatgggag tgtaataaag ccggttgttt ttcttgtcat atgccatggc
     6721 tgctacgccg gtagcaaaag ctgcatttac ataatttcca aagcggctgt cctgcaatgg
     6781 tgcttcaaat tgtttgcggg tggtggcatc atatgccagt aacgtggcat cattaccact
     6841 tagcagtacc tggctgtatt caccattctg caggtttatt ttccgtaaaa aactccagtt
     6901 ggcaccacct tgttgtatat ccgtaacagc ataggcaaaa cgatcagcct gctgggcggt
     6961 aagggtagaa gtcagtaaaa gggctcccga aaaaagggag aaaaagggta gacattttct
     7021 catacacaac ggtttaagag tttacaatcg gataaagtga aatttaaatt acgatttttt
     7081 ccaaaccggt aaaagaaaat tttatgggcg gcagcttatt ttttgaaggg tttatacatg
     7141 cccgtctgca cctgcctgtc tgccggtaca acggtcaaag gttctgcagg ctccaggtac
     7201 tgaaaacatt cagcgggtaa ctgctggtac agtttggttc cttctgtctt ccaggctatc
     7261 atttcatcag atacttcttt gataatctta ccggggttac cggctaccag gctgcggcgg
     7321 gggatttttt catcttgttt gataaagcaa agtgcaccaa caatgcattc atcacccagt
     7381 tctgcattat ccattacaac ggcattcatt cccaccaaac aatttctgcc aatcgcagca
     7441 ccgtgtatga tggcgccatg accaatatgg gcgccttcct ttaatacaac agtcactccc
     7501 ggaaacatat gaatggtgca attctcctgt acattgcaac catcttcaag gatgatttgt
     7561 ccccagtcgc cacgcagtgc agcccccgga ccgatataac agtttttacc aataataaca
     7621 ttgcctgtta cggctgcctg cggatgtaca aatgaagatt cgtcaataac agggatatat
     7681 tctttgaaac ggtagatcat agaatcaagg tttatgcgat gtgccgttta gactgaaata
     7741 agttttttgt cagtgcggta acaaagtccc ttaaactggg caacaacatg atcatgctga
     7801 ttgaagatag caatgtgata aagacctgtt gattttccgt tatgcatttc tttggcctca
     7861 gctgtcagta catctcccac atgtaccggc ttaatgaaat taatggaagt atctaatgct
     7921 actgataaaa cattccggtt gttgcaggca aaggcaaagg cgctgtcggc cagtgaaaag
     7981 gcaatgcccc catgaata
//
LOCUS       DAORLS010000133         7960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4931_length_7960_cov_1.558001, whole genome shotgun sequence.
ACCESSION   DAORLS010000133 DAORLS010000000
VERSION     DAORLS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_7960_cov_1.558001"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK977_10835"
     CDS             <1..480
                     /locus_tag="PK977_10835"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF18659.1"
                     /translation="TAYPQYALEGFEVNALDYLVKPFSFDRFLKAAMKAKEFYEVRET
                     NQQEGKGSDFFFIKADQKLVKIFFDDILDAEALQNYVVIHTTTNKYITYLTFKSVEDY
                     LSSDKFLKVHKSYIVAASKIDSIEAGDIRIGQAHIPISRNQKEEVMDKLLKNRFLKR"
     gene            593..1573
                     /locus_tag="PK977_10840"
     CDS             593..1573
                     /locus_tag="PK977_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRF18660.1"
                     /translation="MLSSYFCAKAQNSPAELSAILTKNCTTDKQKVSAIFNWITENIS
                     YKIRPFQKMPVIGNSHLRWARDYQTIFPDSVELEPLNERVSREVLWSRQAVCDGYAKL
                     FTTLCTYAGIRSEIIVGYARGSLNKPTVKFGVNHYWNAVFFDNDWHLLDVTWASGYID
                     PKKKEFVKELDQNYFLSNPEKFIKDHYPDDPRWTLLPDTKIPDEFRYSPFRQRSFNKY
                     HITAYYPSAGVINVTQGDTILIELEMNFSEDRKISPSELTDSTLFSYSASWIFLNPVK
                     TDRRENGVKRCFYYYPVKSTQVNWLFLVYNNDLVLRYRVKVSDIRSTASL"
     gene            complement(1556..1705)
                     /locus_tag="PK977_10845"
     CDS             complement(1556..1705)
                     /locus_tag="PK977_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18661.1"
                     /translation="MSLLNQNKKGKKKDDKKSKAASGQSSKFIQKPKSGFVSKQQNTG
                     SQRGS"
     assembly_gap    1814..1978
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1979..>2282)
                     /locus_tag="PK977_10850"
     CDS             complement(<1979..>2282)
                     /locus_tag="PK977_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18662.1"
                     /translation="GQEWKTFSDTSVLFTAKYPANWTTKMKEGKRVFFTSPADSPSDD
                     FLENINISVTTNPGYGTEIKIKDLFPSVTNSLKPAFNEFKEERKIEFIFVDEKNEAL"
     assembly_gap    2283..2379
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2502..2984
                     /locus_tag="PK977_10855"
     CDS             2502..2984
                     /locus_tag="PK977_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18663.1"
                     /translation="MKKILLCLTCIYALASSGQQGFDNQQYPVKGNLVKVKDWGSRAL
                     MILSDNYFAATQDSIIFQVGQQEFDELKSRCSASGWPKGLYVSGLSDEEDAAFDQKLN
                     DLKMYQIAVYTHIYNGKTFDRYMILRVPYEENKNWDPEVKWVGNVYFILKEADVEIMK
                     "
     gene            3047..>3459
                     /locus_tag="PK977_10860"
     CDS             3047..>3459
                     /locus_tag="PK977_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF18664.1"
                     /translation="MADATNKEEKSLNFLEEIIEEHLNNGTYTSIVTRFPPEPNGYLH
                     IGHASSICLNFGLTQKYPGYTNLRFDDTNPVTEETEYVESIKDDVRWLGFEWKYERYA
                     SDYFDTLYEYALKLIDKGLAYVDDSTSEEMAAMKGT"
     assembly_gap    3460..3470
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3471..>3849
                     /locus_tag="PK977_10865"
     CDS             <3471..>3849
                     /locus_tag="PK977_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF18665.1"
                     /translation="TEPGKDSPYRNRSVEENRDLFVRMKNGEFQDGARTLRAKIDMAH
                     INMLMRDPILYRIKHAHHHRTGDIWCIYPMYDFAHGQSDSIEEVTHSICTMEFVPHRE
                     LYDWLIEKLEIYPSHQYEFARRNI"
     assembly_gap    3850..3929
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3930..>4219
                     /locus_tag="PK977_10870"
     CDS             <3930..>4219
                     /locus_tag="PK977_10870"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582245.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRF18666.1"
                     /translation="PTISGLRRRGYTPESIREFCDRIGVAKRDNMVDVGLLEFCVREH
                     LNKIALRRMVVFDPIKVVITNYNGSGEVLQSENNPEDANAGLREIPFSREIF"
     assembly_gap    4220..4306
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4307..4732
                     /locus_tag="PK977_10875"
     CDS             <4307..4732
                     /locus_tag="PK977_10875"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRF18667.1"
                     /translation="YIIKCEQVIKAADGSIVEIHCTYMPESKSGSDTSGINVKGTLHW
                     VSAASALEVEVREYDRLFRVEYPTDEEGDFKDYINPGSLKVVKGYAEPALAAAKPEDR
                     FQFLRKGYYCLDKDATDEKLIFNRTVTLKDGWAKEVKKG"
     gene            4853..>5221
                     /locus_tag="PK977_10880"
     CDS             4853..>5221
                     /locus_tag="PK977_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18668.1"
                     /translation="MSYISITTIGTRHSDAIRGLNFYREEFKYMKIRLDEVASKNTSF
                     EARQGLEHFQNQFDIQEKNIHDLKHKIDEHVQSMGYDAQSHQGRVDQEHKETERELMD
                     EYAQLEKVINEVRHEFNNFLS"
     assembly_gap    5222..5327
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5328..>5862)
                     /locus_tag="PK977_10885"
     CDS             complement(<5328..>5862)
                     /locus_tag="PK977_10885"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HRF18669.1"
                     /translation="YGLGVYSTDYAGKQVYWHTGGAFGHVTNVCFVPEENLGITILTN
                     NDNQNFFEALRYQLLDAYLGVPYTDRSKFQWGFFEQNKKLKDAELVALKERVDRKNQP
                     SISLEDFTGDYFNTVYGRVTISKSGNMLICRLQHHPELTGFMEYMDNNEFRLTYSNIG
                     YGVHAVKFSFRDGKPVGI"
     assembly_gap    5863..5924
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5925..6192)
                     /locus_tag="PK977_10890"
     CDS             complement(<5925..6192)
                     /locus_tag="PK977_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF18670.1"
                     /translation="MPLEMKNTYMNTAGLAKRNNVAFPHNNLYSSITKIPFDNVDNLG
                     PATSMVSNVRDLTKWLLLQLDSGRYQGKQVLPWPVLQKTRDINIL"
     assembly_gap    6245..6267
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6268..6814)
                     /locus_tag="PK977_10895"
     CDS             complement(<6268..6814)
                     /locus_tag="PK977_10895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF18671.1"
                     /translation="MRSIQLIVLTLLFSGKVCLAQNPSFITDSLDSYIKRGMSQWQIP
                     GLAIAIVKDGKVILEKGYGIREIGKEDKVDENTLFIIASNSKLFTGTAIAKLDHEKKL
                     SLNDRITQYIPWFRLYDSTSTKLANVRDMLCHRLGTKTFQGDFTFWDSNLQKDSIIWK
                     MRYLKPSGEFRQDYGYCNSAFL"
     gene            complement(6899..>7960)
                     /locus_tag="PK977_10900"
     CDS             complement(6899..>7960)
                     /locus_tag="PK977_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18672.1"
                     /translation="KFVFSDRYTNTNSLGNDFPVDAFNGKYTFKSTASQRTDPSLQDG
                     AVANIVYEVDNAASGTIYTATSSSVIMNPKAFQKADQLAGFSNTAIHAGNDSVQLVIG
                     IANIARNSGNGRSGSVIGIQNVAIQNGNNNNTGELIGFRNIVGRAGATGGRVTGNVYG
                     WFGSFTGLANNVDGNIYGVFLSNVTGAAGSKNFAFYSNRGVNRFGDSVVITTGPATVA
                     RAVLDVNATSAMIVPTGTTAQRPASAVAGMVRYNTDEGGRLETYNGAAWGGIISGGIG
                     IDPPNIPVNSGTTVTFAFTGATVGSVVAISPSSALPNGIIIAWARVSAANTIQVRFEN
                     NAGAAVNPPSIGYNIRVIQ"
BASE COUNT         2196 a         1377 c         1566 g         2190 t
ORIGIN      
        1 acagcatatc cacaatatgc actggaagga tttgaggtaa atgccctgga ttacctggta
       61 aaaccttttt cttttgacag gtttttaaaa gcagctatga aggctaagga gttttatgaa
      121 gtgagggaaa caaaccagca ggagggaaaa ggctcagatt tctttttcat taaagcagac
      181 caaaaactag ttaaaatttt ctttgatgac atattagatg ccgaagcgct tcagaattat
      241 gttgttattc atacaacaac caataagtat attacttacc tgacgtttaa atctgtggaa
      301 gactatcttt cctccgacaa gtttttgaaa gtacacaaat catacattgt tgctgcttct
      361 aaaattgata gtattgaagc cggcgatatc cggattggcc aggcacatat accgataagc
      421 aggaatcaga aggaagaagt aatggataag ctgcttaaaa acaggtttct aaagcggtga
      481 gttgaatagt ttataattta tatcatgttt gcgatctaaa taattcagta ttcatttgaa
      541 ttaattttaa atttaattga cttgaagaat gaggactttt ttaataatat taatgctatc
      601 gtcttatttc tgtgctaaag cccagaatag tccggcagaa ctttctgcta ttttgacaaa
      661 aaactgcaca acagataaac aaaaagtgtc agctattttc aactggatca ctgaaaatat
      721 ttcttataaa ataaggccct ttcaaaaaat gccggtaata ggtaattctc atttaagatg
      781 ggcaagggat tatcaaacaa tctttccaga ctcagttgaa ttggaaccct taaatgaaag
      841 ggtgtcaaga gaagtgttat ggagccgaca ggctgtatgt gacgggtatg ctaaactgtt
      901 tacgacttta tgtacatatg ctggtatcag gtctgagatt attgtagggt atgcccgggg
      961 tagcctaaat aagccaacgg taaagtttgg ggtaaatcat tactggaatg cagttttttt
     1021 tgataacgac tggcatttgc ttgatgtgac ttgggccagt ggttacattg atcctaaaaa
     1081 gaaagaattt gtaaaagaac ttgatcaaaa ctatttttta agcaacccag aaaaatttat
     1141 caaggatcat tatccagatg acccaagatg gacgttgctg ccggatacca aaattccgga
     1201 tgagttcagg tattcacctt tcaggcaacg gtcttttaat aagtaccaca taacagctta
     1261 ttatccttca gcaggcgtaa ttaatgtaac gcagggagat actattttaa tagagctgga
     1321 aatgaacttt tcagaggacc ggaaaatttc accaagtgaa cttactgatt caaccctttt
     1381 cagttattct gcctcatgga tatttcttaa tcctgtaaaa acagatcgaa gagaaaacgg
     1441 ggtcaaacgt tgtttttatt attatcctgt caagtcaacc caggtaaatt ggttgtttct
     1501 tgtctataat aacgatcttg ttttgcgcta tagggtaaaa gtcagcgata tcaggtcaac
     1561 tgcctctttg tgatcctgtg ttttgctgct tacttacaaa accggattta ggtttttgta
     1621 tgaatttgga gctttgtcct gaggcggctt tactcttctt atcgtctttc ttttttccct
     1681 ttttgttttg attcaggagc gacatattta ttgttttata tctttaaaat ttgaagttat
     1741 tttcagctta tgcagctatc tgtcaaattt atgattttct atttttaaaa ttaatccggc
     1801 agtttcaatt cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     1981 aggcttcatt tttttcatca acaaatataa attcaatttt tctttcttct ttaaattcat
     2041 taaaggcggg ttttaaacta ttggttacgg aagggaacag atctttaatt tttatttctg
     2101 taccataacc aggattggtt gtaacgctga tatttatgtt ctcgagaaag tcatctgaag
     2161 ggctgtcggc aggtgatgta aagaaaaccc ttttcccttc tttcattttt gtggtccagt
     2221 tggctgggta ctttgccgtg aataagacag aggtatcaga aaatgtcttc cattcctgtc
     2281 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaaacggtaa taaagataca
     2401 catgaaagca ccttgttttg agttcagaac tgcaactaac caaaaatacc cgccaatggg
     2461 tacaggaaat gcgtttatta gggcgatttt taaaacaaaa aatgaaaaag atacttcttt
     2521 gcctcacatg tatatatgcg ctggcttctt caggacagca gggctttgat aaccagcagt
     2581 atcctgttaa gggcaatttg gtcaaggtga aggattgggg tagccgggcc ttaatgattc
     2641 tgtcagacaa ctattttgct gccacacagg atagtatcat tttccaggtg gggcagcagg
     2701 agtttgatga attaaaatcc aggtgttcgg ccagcggatg gccaaaaggc ctttatgtgt
     2761 ctgggctttc cgatgaagaa gacgctgcat ttgaccaaaa gctgaatgat cttaaaatgt
     2821 accaaatagc cgtttatact catatctata atggtaagac gtttgaccgg tatatgatcc
     2881 tgagggttcc ttatgaagaa aataagaatt gggacccgga ggttaaatgg gtcggtaatg
     2941 tttattttat attaaaggag gcagatgttg aaataatgaa ataaggcgac cccgtactgt
     3001 aacggaatgc cgaggcctgc ggcataatta ttatctttgc aggcttatgg cagacgcaac
     3061 aaacaaagaa gaaaagagcc ttaattttct cgaagaaata atagaagaac atcttaacaa
     3121 cgggacgtat acaagtatcg ttactcgttt tcccccagaa cccaatggtt acctccatat
     3181 tggtcatgct tccagcattt gcctcaattt tgggctaaca cagaaatatc ccggctatac
     3241 taatctacgg tttgacgata ccaacccggt tactgaagaa acggaatatg tggaaagcat
     3301 caaggatgat gttcgctggc ttggctttga atggaagtat gaacgttatg ccagtgatta
     3361 ttttgataca ttgtatgagt atgccctgaa gttaattgac aaaggacttg cctatgttga
     3421 tgacagtact tccgaagaga tggctgccat gaagggcacn nnnnnnnnnn tactgaaccg
     3481 ggtaaagaca gcccttatcg taacaggtcg gtggaagaga acagggatct ttttgtaaga
     3541 atgaagaatg gcgagttcca ggatggggca agaacgctcc gggcaaagat tgatatggca
     3601 catatcaata tgctgatgag agatccgatt ctctaccgta taaagcatgc acatcatcac
     3661 cgcactggtg atatatggtg tatttacccg atgtatgatt ttgcacacgg ccaaagtgat
     3721 tcgatagagg aagtgacaca ttccatatgt accatggagt ttgtaccgca tcgtgaatta
     3781 tatgactggc tgattgaaaa gctggaaatt tacccttctc atcaatatga atttgccagg
     3841 agaaacattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccgacaatta gtggtttgcg cagaagagga
     3961 tacacaccgg aaagtatccg tgaattctgc gacaggattg gtgtggccaa acgggataat
     4021 atggtggatg tgggattact ggaattctgc gttagggagc atttgaacaa aattgctctt
     4081 cgcaggatgg tcgtttttga tccaatcaaa gtagttatta caaattataa tggtagtggt
     4141 gaggttttgc aaagcgaaaa caacccggaa gatgcaaatg ccggtctgcg ggaaattccg
     4201 ttcagccgtg agattttcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntata ttattaagtg
     4321 tgaacaggtg ataaaagctg cagatggaag tattgtggaa attcactgca cctatatgcc
     4381 agaaagcaag agtggttcag acaccagtgg tatcaatgta aagggaacgc tgcattgggt
     4441 gagtgctgct tcagctcttg aggtagaagt aagggaatac gatcgtcttt tcagagtaga
     4501 gtatcctacc gatgaggaag gagattttaa agattatatt aatcccggga gtttaaaagt
     4561 ggtgaaggga tatgcagagc ctgcattagc tgctgccaag ccggaagaca gattccagtt
     4621 cttacgtaag ggatattatt gccttgataa agacgccaca gatgagaaac taatctttaa
     4681 caggacagtt accttaaaag atggttgggc taaagaagta aagaaagggt aggtgccgcg
     4741 attctttaat gaccaatttt cagtttaaac ggcagccaaa tcagccccca aactgattta
     4801 tatcagatga tttgccttag ctgcttaata gcttcggtta aaacttaata ccatgagcta
     4861 cataagcatt accactattg gtacccgcca ttccgatgca attagaggtt tgaatttcta
     4921 cagggaggaa ttcaagtaca tgaagattcg tttggacgaa gtcgcatcaa agaatacaag
     4981 ttttgaagcc cgccagggcc ttgaacattt tcagaaccag tttgatatcc aggaaaagaa
     5041 tatccatgac ctgaaacata agattgacga acatgttcag tctatgggtt atgatgccca
     5101 atcacaccag ggcagggtag accaggagca taaagaaaca gaaagagagc ttatggatga
     5161 atatgcgcaa ctggaaaaag taataaatga agtaaggcat gagtttaata atttcctgag
     5221 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa ttccaactgg
     5341 tttaccatcc cggaaagaga atttgaccgc atgtacacca tagccaatgt ttgaataggt
     5401 taacctgaat tcgttgttgt ccatgtactc catgaagcct gtcaattcag gatggtgttg
     5461 taagcggcaa atcagcatat tgccggattt ggagatagtt actctgccgt aaacagtatt
     5521 aaaataatcg ccggtaaagt cttcaagact aatggacggt tgattttttc tgtcaactct
     5581 ttcttttaat gctaccaatt ctgcatcctt tagttttttg ttttgctcaa agaagcccca
     5641 ttgaaatttg ctcctgtctg tataaggaac gccaaggtag gcatcaagca gctggtagcg
     5701 taatgcctca aagaagtttt gattatcgtt attggtcagg atagtaatgc ccaggttttc
     5761 ttccgggaca aaacatacat tcgtgacatg cccaaacgcc ccgcctgtat gccagtacac
     5821 ctgttttccg gcataatcgg ttgaatatac acccaatcca tannnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcagga tattaatatc
     5941 tcttgttttt tgtaaaacgg gccagggtaa aacctgtttt ccctggtacc gtccgctgtc
     6001 taattgtaag agcagccatt tggtaagatc tcgtacattg cttaccatac tggtagctgg
     6061 accaaggttg tctacattat caaagggaat ctttgtgatg gagctataaa gattgttatg
     6121 ggggaaggca acattgtttc tctttgccag ccccgctgta ttcatatagg tatttttcat
     6181 ctctaagggc ataagaatat tttcttccac atatttttcc catgtctgcc ctgtgacttt
     6241 ttctnnnnnn nnnnnnnnnn nnnnnnncca ggaaggcgga gttgcaataa ccatagtcct
     6301 ggcggaactc accggagggt tttaaataac gcattttcca gatgatagaa tctttttgga
     6361 ggttagaatc ccagaaagtg aaatctcctt gaaatgtttt agtacctagc cggtggcaaa
     6421 gcatatctct tacattggcc agttttgtag aagtactgtc atataacctg aaccagggta
     6481 tatattgtgt tatcctgtca ttcagagaca gttttttctc atgatccagt ttggcaatag
     6541 ctgttcctgt aaaaagtttt gagttagatg caatgatgaa caaggtgttt tcatctactt
     6601 tatcctcttt gcctatttca cggataccat atcccttttc gagtatgacc tttccatcct
     6661 ttacaatagc aatggctaat ccaggtattt gccattgact cattcctctt ttaatataac
     6721 tgtcaaggct atcagtaata aaggaaggat tttgtgcaag acaaactttg ccagagaaaa
     6781 gcaatgtcaa aacgattaat tgaattgatc tcatataatt gaattgaaat ctgaaagtac
     6841 atataaaatg aaaaatgccg gcttaaaggc cggcatttaa caagtaatag ctgaaacatt
     6901 attgtatcac cctgatatta tatccaatcg agggcgggtt gaccgctgcg ccggcgttat
     6961 tttcaaacct aacctggatt gtattagctg cactcactct tgcccatgca atgataattc
     7021 cattgggtaa agcacttgaa ggtgaaatag ctacaacact tcctactgtt gcaccggtaa
     7081 aggcaaaagt cactgttgtt cctgaattga ccggaatatt aggagggtca attcctatac
     7141 caccgctaat aataccaccc caggcagcac cgttgtatgt ttcgaggcga ccgccttcat
     7201 cggtattgta acgaaccata cctgctacag cgctggcagg cctttgggct gttgttccgg
     7261 tgggaacgat catggcactg gtagcattca catctaatac agccctggca accgtggcag
     7321 gcccggtggt tataactacg gaatcaccaa aacgattaac tccccggttt gaataaaaag
     7381 caaagttttt gctgccagct gctccggtta cgttagaaag aaatacacca taaatatttc
     7441 catcaacgtt gttggcaagc ccagtaaagc tgccaaacca gccatataca ttgcccgtaa
     7501 cccgtccacc ggtagcacct gctcttccaa cgatattacg gaaaccaata agttcacctg
     7561 tattattatt gttgccgttt tgtatggcaa cattctgaat tccgatcaca gaaccggatc
     7621 tgccattacc actgttcctg gcgatgttag caatgccgat tacgagttgt acagaatcat
     7681 ttcctgcatg aatggcagta ttgctaaaac cagctaactg atctgctttc tgaaatgctt
     7741 ttggattcat tatgacactt gacgaggttg ctgtataaat ggtacctgaa gctgcattgt
     7801 caacttcata tacaatattg gcaacagcac catcctgcaa cgagggatct gtcctttgcg
     7861 aagccgttga tttgaaagta tacttgccgt tgaatgcatc tacaggaaaa tcgtttccaa
     7921 gactattggt attggtgtag cggtcgctaa atacgaactt
//
LOCUS       DAORLS010000134         7948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4937_length_7948_cov_2.154441, whole genome shotgun sequence.
ACCESSION   DAORLS010000134 DAORLS010000000
VERSION     DAORLS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4937_length_7948_cov_2.154441"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK977_10905"
     CDS             complement(<1..280)
                     /locus_tag="PK977_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF18673.1"
                     /translation="MNKIFWFLLIIPFFAAAQKNDRRLESTIREAINGYKGDIGIYVK
                     NLRTGKTVSLNADTIFPTASIVKVPILVGIMDKMERKELNYDSVLVYKD"
     gene            complement(300..1601)
                     /locus_tag="PK977_10910"
     CDS             complement(300..1601)
                     /locus_tag="PK977_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF18674.1"
                     /translation="MQKSLAASLLMVCSFLASAQSVADKLKTKAFLAADKIEPKCIEW
                     RRDFHQNPELGNREFRTARIIAAHLKSLGIETTENVAKTGVIGILKGGKPGPCIALRA
                     DIDALPVTERVNIPFASKQKTTYNGQEVGVMHACGHDTHTAILMSVAEILSGMKNELA
                     GTIKFVFQPAEEGPPEGEEGGAALMVKEGVMDNPKVDAIFGLHIMSDIEAGKIEYKPG
                     AYMASADWFTIKVKGKGSHGSKPWKGVDPIQTAAQIIEGLQNIVSRQMELTKAPVVIT
                     VGKINGGVRQNIISEECEMSGTIRTLDSKMQSDVHQRIKWTAEKIAEANGATADVTID
                     TKTLVTYNTPELVKKMLPSFESAAGAGNVKEGEWTTGAEDFSYYGTKAPALFFFLGGS
                     PKGKDPKSAPPHHTADFFVDEVGMKTGIRAFCSLVIDYMYN"
     gene            1688..2929
                     /locus_tag="PK977_10915"
     CDS             1688..2929
                     /locus_tag="PK977_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRF18675.1"
                     /translation="MLLRHLPFLRAVLLHSVTAFGGPQAHLGMMMKTFVHCKPYVTEQ
                     ELMEYNAFCQLLPGASSTQTLTLIGYKRGGVPLAVVTLIIWILPASILMGGLSFLLQY
                     IDQRELGTDIFKFIAPMAVGFLAFAAFTAFPLAVNNFITWVIMLTGAAITYFLFGQAW
                     VIPAVIIASGIVTNISRKRIPQTEQRPRKIKWWNFWLFGIIFITAGIVSEMARKNDWP
                     NRRPINLFENTYRMGSLVFGGGQVLIPMMYEQWSVRPEVIKNKNPNAVQIDENDLYTG
                     MGIVRAMPGPVFSIAAFTGGMALKDMGTEMQVLGCLIGMIAIFLPSALLVLFFFPIWN
                     NLKKYAAVYRSLEGINAAVVGIMVASTIYITKDISITELKTVSLLNVGVMLGTFLLLN
                     FTRIAPPFIVLACLLLGFISF"
     gene            complement(2930..3502)
                     /gene="nadD"
                     /locus_tag="PK977_10920"
     CDS             complement(2930..3502)
                     /gene="nadD"
                     /locus_tag="PK977_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRF18676.1"
                     /translation="MKLGLYFGSFNPVHVGHLIIANHFLNETDLEKVWFIVSPHNPLK
                     NEKGLLNEFHRLQLVRLATEDDNRIKVSDIEFSLPKPSYTINTLAYLTEKNPEHEFCI
                     IMGSDSFQNIDKWKNYEAIIKNYPVYVYPRPGFEISNPIKADLKIVTAPLLEISATLI
                     RNNIRSGKSVRYLLPDKVLEEIEKGGYYKK"
     gene            complement(3518..3748)
                     /locus_tag="PK977_10925"
     CDS             complement(3518..3748)
                     /locus_tag="PK977_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRF18677.1"
                     /translation="MIVKLSELKPGQEGTIKEFNSHDIFLKLMEMGCVPGEKILMEQI
                     APLGDPVSVQVAGYHLSLRLNEADQILIELNN"
     gene            3810..4241
                     /gene="ybeY"
                     /locus_tag="PK977_10930"
     CDS             3810..4241
                     /gene="ybeY"
                     /locus_tag="PK977_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRF18678.1"
                     /translation="MAYVGTIHFFFPLKTNLTNRTKLKVFLERIFRKEGKALEKLNYI
                     FCSDKELLRINQDYLGHDYYTDIITFDLSGNNKATMAEIYISVERVKDNAGQLGVPFK
                     EELHRVIFHGVLHLCGYGDKNENLARIMREKENFYLNQYFK"
     gene            4353..>4548
                     /locus_tag="PK977_10935"
     CDS             4353..>4548
                     /locus_tag="PK977_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18679.1"
                     /translation="MFPEYDVIVVGAGHAGCEAAAAAANLGSKTLLVTMNMQTIAQMS
                     CNPAMGGIAKGQIVREIDAIG"
     assembly_gap    4549..4559
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4560..6223
                     /gene="mnmG"
                     /locus_tag="PK977_10940"
     CDS             <4560..6223
                     /gene="mnmG"
                     /locus_tag="PK977_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRF18680.1"
                     /translation="SGIVTDLSTIQFRMLNRSKGPAMWSPRAQNDRMLFHLKWREMLE
                     KTPNLEFYQDMVKGLLIKDNRCCGVITGMGHEIRSKSVVLTNGTFLNGIVHIGEKNFG
                     GGRMAEKAATGITEQLVSLGFESDRLKTGTPPRIDGRSLDYSKMEEQPGDDEVLGFSF
                     LDIEKPKTQRSCWITYTNQSVHDILKTGFDRSPMFAGRIDGVGPRYCPSIEDKINRFA
                     ERERHQLFVEPEGWNTIEIYLNGFSTSLPEETQYEALRNIPGFENARMFRPGYAIEYD
                     FFPPTQLNYSLETKLIQNLFFAGQINGTTGYEEAACQGLMAGINAHQKAKELEPVILK
                     RSDAYIGVLIDDLISKGTQEPYRMFTSRAEFRTLLRQDNADLRLTELSYRMGLASQER
                     MDRVKNKRESVEKIKTVLKEMAIEPDEINGWFGQINSSPIIEKQKAQKILLRPDVELQ
                     EFAVAIPRLKEALSPFTREAIEQAAIQVKYDVYIEKEKELVHRMSQLEELEIPETFDY
                     KKIASLGNEAREKLTRIKPRTLGQASRISGINPSDVQILMVFMGR"
     gene            6331..>6846
                     /locus_tag="PK977_10945"
     CDS             6331..>6846
                     /locus_tag="PK977_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18681.1"
                     /translation="MKKNIFLFAIVSLGFLFSNTKTEAQQYKFRQVTNMMGMKMETTI
                     YVKGMRKRTEPGSMMGMPASPITIEQCDLQRTIKINDKKKLYYIEPFAKDDEEIIDED
                     VKTVPKTKTPPKPTTTQKGGIIYISYSITDTGERKKMYGFNARHVWTSNKMVPSPESC
                     TMKDSLLIKTDG"
     assembly_gap    6847..6856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6857..7662
                     /locus_tag="PK977_10950"
     CDS             <6857..7662
                     /locus_tag="PK977_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18682.1"
                     /translation="YIDLPQFNCPVNYKRYNNPQQTDRQKPECTDKFVTRRRGKGKLG
                     FPLIETTTIIMGDGKKSTSQFETNLETLELTTAKLDSMLFEIPIGYTETKNEQDLQDK
                     LDINDIMNQYKKNNNNNDNDKPAANDPKGEGKIRIGVLVPTGDEQVNAADLQQYLAGI
                     LISGTVEAVAVSSEEEAKSSQCDYSLATTFVRVKSGSKVGGLLKAVKNADPNAASSFT
                     IEANMLLKSLGDGSVKAQPKIDGKFEGKIDDAAKKAMDDGSRQVLRAIK"
     gene            complement(7718..>7948)
                     /locus_tag="PK977_10955"
     CDS             complement(7718..>7948)
                     /locus_tag="PK977_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18683.1"
                     /translation="LVMVVIGVSLLITNKTAGAGIEAGSLVGIYSYIQRFVSGLDTIP
                     YTVQRLSSLNDITRRIELQEEDLTSPPLKEVA"
BASE COUNT         2375 a         1562 c         1742 g         2248 t
ORIGIN      
        1 aatctttata aaccagtaca gaatcatagt tcagttcttt tctttccatt ttatccataa
       61 tgccaacgag gatcggcact tttacaatac tggcagtcgg aaatatagta tcagcattca
      121 atgaaactgt ttttcctgta cgaagatttt ttacataaat gccaatatca cccttgtagc
      181 cattaatagc ttcacggatt gttgattcca ggcgacgatc atttttttga gcagcagcaa
      241 agaatggaat gatcagtaaa aaccagaaaa tcttgttcat attaccaaag ggaagattgt
      301 cagttgtaca tataatcgat tactaaactg caaaaggccc gtatacccgt tttcatcccc
      361 acttcatcca caaaaaaatc agcggtatga tgtggtggtg ccgatttagg gtcttttccc
      421 tttgggctgc ccccgagaaa gaaaaaaaga gcaggtgctt ttgtgccata gtaggaaaaa
      481 tcttcggccc cggtggtcca ttcgccttcc tttacattac cggctcctgc agcggactcg
      541 aacgagggaa gcattttctt taccagctcg ggtgtgttat aggttaccag cgttttagtg
      601 tcaatcgtta catcggcagt agcgccattg gcttcggcaa ttttttcggc cgtccatttt
      661 attcgctgat gtacgtctga ttgcatttta ctgtccaatg tccggatggt tccgctcatt
      721 tcacattctt ctgatatgat attctggcga acaccaccgt ttatttttcc gactgtaatt
      781 acaaccgggg ctttggtcaa ttccatctgg cggcttacaa tattttgcaa gccttcgatt
      841 atctgtgcag ctgtttgtat ggggtcaaca cctttccagg gttttgaacc atgacttcct
      901 ttgcccttta ctttaatagt aaaccagtcg gcagatgcca tataagcccc gggtttatac
      961 tcaatttttc ctgcttctat atccgacatg atatgcaacc cgaaaatagc atccacttta
     1021 ggattgtcca ttacaccttc tttgaccatc agtgcggcgc ctccttcttc accttcgggg
     1081 ggaccctctt cagctggttg aaaaacaaac ttaattgtac cggccagttc gtttttcata
     1141 cccgataaaa tttctgcgac agacatcagt atagcggtat gtgtgtcgtg cccgcaggca
     1201 tgcattacgc caacctcttg cccgttgtaa gttgtttttt gtttggatgc aaacggaata
     1261 ttaactcttt cggttactgg taacgcatct atatctgcac gcaaggcgat gcagggccct
     1321 ggcttacccc cttttaatat acctattaca ccagtttttg ctacattctc agttgtttct
     1381 atgcccagac tttttaggtg tgcagctatg atcctcgctg tgcgaaactc cctgttgcct
     1441 aattcggggt tttgatgaaa atcccttcgc cattctatac atttaggttc aattttatct
     1501 gcggccaaaa aggcttttgt ttttagttta tcggcgaccg attgagcaga cgccagaaaa
     1561 gagcaaacca ttaacaaaga agcagcgaga cttttttgca taagtaaatt tttatgaatt
     1621 tactgtaatt gagcttttgt cagtgattaa tactgccttt catgaatttt tttatattta
     1681 aagcccaatg ctgctgcgac atctaccatt tctaagggct gtgttgttac acagtgttac
     1741 tgcttttggc gggccacagg cacatttggg tatgatgatg aaaacctttg ttcactgtaa
     1801 gccctatgtt acagagcagg aacttatgga gtacaatgca ttttgccagc tattacccgg
     1861 ggcatcctct acacagacac tgaccctgat agggtacaaa agaggagggg tgccgcttgc
     1921 cgtcgttaca ttgataatct ggatattgcc agccagtatt ttgatgggag gactttcgtt
     1981 cttgttacag tatatcgatc agcgggagct tggcacagat attttcaagt ttattgcgcc
     2041 catggctgtc gggtttcttg cttttgctgc attcacagcc tttccccttg cggtaaataa
     2101 tttcattaca tgggtgataa tgctgacagg agcggccatt acctattttc tatttggaca
     2161 ggcatgggtt atacctgccg tgataattgc cagcggaatt gttaccaata tcagccgtaa
     2221 gcgaattccg caaacagaac aaaggccaag aaaaataaaa tggtggaatt tttggctttt
     2281 cgggatcatt ttcatcacag cgggaattgt gagtgaaatg gcaaggaaaa atgattggcc
     2341 caacagaaga cccatcaatt tgtttgaaaa tacgtatcgt atgggaagcc ttgttttcgg
     2401 aggaggacaa gttttgatac cgatgatgta tgagcagtgg agtgttcgtc cggaggtgat
     2461 taaaaataaa aatcccaatg cggtacagat cgatgaaaat gatctttata cagggatggg
     2521 aattgtacgg gcaatgccag gccccgtctt ttccatcgca gcttttaccg gcggcatggc
     2581 tctgaaggat atgggaaccg agatgcaggt attgggttgc ctgattggaa tgatagcgat
     2641 ctttcttccc agtgcactgc tggttttgtt tttctttccg atctggaaca acttaaaaaa
     2701 atacgcagct gtttaccggt ctttagaggg aatcaatgcg gctgtggtag gcatcatggt
     2761 ggcgtccact atttatataa caaaggatat ttcaattact gaactgaaaa cggtcagcct
     2821 gcttaatgtc ggggttatgc tgggcacatt tctattactg aattttacca ggattgctcc
     2881 gccgtttatt gtattggcct gtctgctgct gggttttatt tctttctgat catttcttgt
     2941 aataaccacc tttttcaatt tcttccagca ctttatccgg aagtaaatag cgtacagatt
     3001 ttcctgatcg tatattatta cgtattagtg tagccgaaat ttccaataac ggggccgtta
     3061 ctattttcag atcagctttt atgggattac ttatctcaaa tccggggcgg ggataaacat
     3121 aaaccgggta gttttttatt atcgcctcat agtttttcca tttatcaata ttctggaagc
     3181 tgtcgctgcc catgataatg caaaattcat gttccgggtt tttttccgtg agatatgcca
     3241 gggtgtttat tgtataggag ggcttgggca acgagaattc aatatccgaa accttaattc
     3301 tgttgtcatc ttctgtagcc agccttacca actgcaggcg gtgaaattca ttaagcagac
     3361 ctttttcgtt ctttagcgga ttgtgcggtg aaacaataaa ccaaactttt tcaaggtctg
     3421 tttcgttcag gaaatgattg gctattatca ggtggccaac atgtacagga ttaaaagaac
     3481 caaaatataa ccctaatttc atttaaatat ttgataatca attgtttaat tcgattaata
     3541 tttgatcagc ctcattaagc cgaaggctaa gatggtatcc tgccacttga acagataccg
     3601 gatctccgag aggtgcaatt tgttccatta atatcttttc accaggtaca catcccattt
     3661 ccatcagttt taaaaaaata tcatggctgt taaactcctt gatagttccc tcttgacctg
     3721 gttttaattc ggataacttg acaatcattt ttccattatt accacaaagc taatcttgct
     3781 gatttacatt tgcttacgaa atttgaaaaa tggcatacgt tggcactatt catttctttt
     3841 ttcctttaaa gacaaacctg actaacagaa cgaaactcaa agtctttcta gaaagaatat
     3901 ttcgaaagga gggcaaagca cttgagaaat tgaattatat tttttgttct gacaaggagc
     3961 tgttgcgtat caatcaggat tacctcgggc atgattatta caccgatatt ataacttttg
     4021 acttatcagg taataataag gcaacaatgg ctgagatata tataagtgta gaacgggtta
     4081 aggataatgc cgggcaattg ggagttcctt ttaaggaaga gttgcaccgg gtaatttttc
     4141 atggagtatt gcacctctgt gggtatggag ataaaaatga aaatctggca aggataatga
     4201 gagagaagga gaacttttat ctaaatcaat attttaagta acgtttcacg gggaacgttt
     4261 taggatatac aggttcatgc gtttcccgtg gaacataggt ttgttccaaa ataagctttt
     4321 aaagaggggg ttgaggcgta catttgcacc ccatgtttcc tgaatacgac gtaattgtag
     4381 taggcgccgg tcatgccgga tgtgaagcag ctgcggctgc agcaaatctt ggttccaaaa
     4441 cccttttggt gaccatgaat atgcaaacta ttgcccaaat gagctgtaat ccggccatgg
     4501 gcggtattgc gaagggtcag attgtgagag agatagatgc catcggcgnn nnnnnnnnna
     4561 ttcgggaatc gtaacggatc tttcaactat ccaattcaga atgctgaaca ggagcaaagg
     4621 gcctgctatg tggagtcccc gggcgcagaa tgaccggatg ctctttcacc tgaagtggag
     4681 ggaaatgctg gaaaaaacac ccaatcttga gttttaccaa gatatggtaa aggggctttt
     4741 aataaaggat aacagatgtt gtggagttat taccggcatg ggacatgaaa tcaggagcaa
     4801 atctgtagtg ttgaccaacg gaacctttct caatggaatt gtacatatcg gcgaaaagaa
     4861 ttttgggggt ggaagaatgg ctgaaaaggc tgcaacaggt attaccgagc aattggtttc
     4921 tttaggattt gagagtgacc gattgaaaac cggtacgccg ccccgaatag atggccgaag
     4981 cctggattat agcaagatgg aagaacaacc gggagatgat gaggtgcttg gcttctcttt
     5041 cctggatata gaaaagccaa aaacacaaag aagttgctgg attacctaca ctaaccagtc
     5101 agtacatgat attcttaaaa caggctttga ccgtagcccg atgttcgcag gcaggataga
     5161 cggagtgggc ccccggtatt gtccaagcat agaagataag atcaaccgtt ttgcagaaag
     5221 ggaaaggcac caactttttg tagaaccaga gggatggaat accatagaaa tatatttaaa
     5281 tggcttctcc acatccttgc cggaagaaac tcaatacgaa gcgcttcgta atatacctgg
     5341 ttttgagaat gcccgtatgt tcaggcctgg ttacgctatt gaatatgatt tctttccgcc
     5401 cacacagctt aattattcat tggaaaccaa gctgatacag aatctttttt ttgcgggaca
     5461 aataaacggc accaccgggt acgaagaagc ggcctgccag gggctaatgg ccggaatcaa
     5521 tgcccaccaa aaggcgaaag agttggagcc ggtcatttta aaaaggagcg atgcctatat
     5581 cggagtattg atagacgacc tgatcagcaa gggcacccag gaaccttacc ggatgtttac
     5641 atcaagggct gaattcagga cattgctgag gcaagacaat gcagacctgc gtttgacgga
     5701 attaagctac aggatgggcc ttgcatcgca ggaaagaatg gaccgggtga aaaataagcg
     5761 agaatcggtt gagaaaataa aaactgtttt aaaagaaatg gcgatagagc cggatgagat
     5821 aaacggatgg tttgggcaaa tcaattcttc tccgataatt gaaaaacaaa aagcacaaaa
     5881 gatacttctg cgtcctgatg tggaactcca ggagttcgcc gtagcaattc caagattaaa
     5941 agaagcacta tcgccattta ccagagaggc cattgaacag gctgccatcc aggtaaaata
     6001 tgatgtttat atagaaaagg aaaaagagct ggtacatcga atgtctcaat tggaggaact
     6061 ggaaattccg gaaacattcg attataaaaa aatagcttct ttaggtaatg aagcaaggga
     6121 aaagctcacc cgtataaaac cccgcaccct cggacaggcc agccgcatat ccggtatcaa
     6181 tcccagtgat gtacagatat tgatggtgtt tatgggaaga taaaggcatc aaaaatttcc
     6241 tgtcaattta gttctccctc agtatataag ttatactgaa tagctacctt tagtttatca
     6301 ttattaaaac cattaaacct tcaataaaaa atgaaaaaga atattttcct ttttgcaatt
     6361 gtttcccttg gatttttgtt ttcaaatacc aaaacagaag cacagcaata caaattccgc
     6421 caggtaacga atatgatggg tatgaaaatg gagacaacca tctatgtgaa aggaatgagg
     6481 aaacgaacag aacccggaag tatgatgggc atgccggcca gtcccattac aatagaacaa
     6541 tgcgacctgc aaagaaccat taagatcaat gataaaaaga agctttatta tatagagcct
     6601 tttgcaaaag atgatgagga aattattgat gaggatgtaa agactgtacc gaaaacaaaa
     6661 acgcctccca agcctaccac tacacaaaag ggaggtatca tttatatttc atatagtatt
     6721 actgatactg gtgagagaaa aaaaatgtac ggctttaatg cccgccatgt atggaccagc
     6781 aataaaatgg taccgtcccc cgaatcctgt actatgaagg acagcctgct tataaaaaca
     6841 gatggcnnnn nnnnnnggta cattgacctt ccacaattca actgtccggt taattacaaa
     6901 agatataaca atccgcagca aactgaccga caaaagccgg agtgtacaga caaatttgta
     6961 acccgtagac ggggtaaagg aaaattaggc tttccgctta ttgagacaac caccattata
     7021 atgggtgatg gtaaaaaaag taccagccag tttgaaacca atctggaaac attagaattg
     7081 acaaccgcca aactggattc aatgttattt gaaattccta ttggatatac agaaacaaaa
     7141 aacgagcagg acctgcagga taagctggac attaatgata tcatgaacca gtacaagaaa
     7201 aacaataata ataatgataa cgataagcca gcggcgaatg atccgaaggg ggaagggaaa
     7261 atcaggatag gagtattggt tccaaccgga gacgaacagg tgaatgctgc tgatttgcaa
     7321 caatatcttg ctggtatatt aatcagcggt actgtggaag ccgtagccgt ttcttcagaa
     7381 gaagaagcca aaagttcaca atgtgattat tcacttgcta caacctttgt aagggtgaaa
     7441 agcggcagca aagtaggtgg tctgctgaaa gcagtaaaaa atgcagaccc taatgcagcc
     7501 tcttccttta ctatagaagc aaatatgttg ttgaaaagcc tgggagacgg atcggtaaaa
     7561 gcacagccta aaattgacgg aaagtttgaa ggaaagattg atgatgctgc caaaaaagca
     7621 atggatgacg gaagccggca ggtgctgaga gccattaagt aaaaaatttt gtaataaaaa
     7681 aggaaagaga actctttcct tttttattgt tatgctttca ggcaacctct ttgagaggcg
     7741 gacttgtaag gtcttcttcc tgcagttcaa tccttcttgt aatgtcatta agtgatgaaa
     7801 gacgctgaac agtatatgga attgtatcaa gcccggatac aaagcgttga atataactat
     7861 agataccaac aaggctaccg gcttcaatac cagccccggc tgttttatta gtaataagca
     7921 gagaaacacc aatgacaacc attaccaa
//
LOCUS       DAORLS010000135         7912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4962_length_7912_cov_2.089729, whole genome shotgun sequence.
ACCESSION   DAORLS010000135 DAORLS010000000
VERSION     DAORLS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4962_length_7912_cov_2.089729"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1149
                     /locus_tag="PK977_10960"
     CDS             88..1149
                     /locus_tag="PK977_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRF18684.1"
                     /translation="MGNELVQRSSRPIAIWLLVGVGMIIIQILLGGITRLTGSGLSIT
                     EWNVVTGTLPPMSEQEWLTEFNKYKATPQFNLLNFDFTLKDFKFIFFWEWFHRFWARL
                     IGVVFFIPFVIFLVQKRFSPSMVKPMIILFLLGALQGAVGWIMVVSGLTGDAIYVKPT
                     RLALHFIFALGLLAYTFWFAMELLVPKKELVINKKARQLNIAIIVVLVIQLIYGALMA
                     GNKAATAAPTWPDINGYAFPPNMMGHGEGLLNLLDNKITIHFIHRGLAYLLLIMVLVL
                     TYRLYKTGATSFLKKLRWWPALLVIIQVVLGILSVLTSLQIVPNQWGVFEWMAQLHQL
                     VAMFLLLSLIALLYSIKSK"
     gene            1593..3188
                     /locus_tag="PK977_10965"
     CDS             1593..3188
                     /locus_tag="PK977_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRF18685.1"
                     /translation="MWFSYAPEVKAVIRFIELLKVRVTRSTISETLQAHSDWPSLLCI
                     SDSLKQWHIPNAAAKIDNADLNQLPVPFIAYLPGQPVPITVVTEIADNTITTYNDNFT
                     RRVALPKEKFLSLWTGIYLLAEPTVKSGEKNYLQKKKRAFVASLTPVLLLILIIGCCI
                     FWLDKNLQIINLSQQAGVWFQYGVVLTGVAVCILLLWYEMDQNNPLLNKVCTGVARGN
                     CSAILTGKASRLFSWLSWSEVGFFFFAGSLFLIVLTPYNPVPYALNLVALPYIFFSVY
                     YQWQVAKQWCVLCLAVQVLLLAGAVNVMIHFLPLLGLFTTKNILQSFAAYLLMPLLWY
                     SIKPLFLNLQGLKGDKRQYLRMKFNTEVFETLLVRQKKITAPTADLGIVIGNPHAQHE
                     IVKVCNPYCGPCAASHPKLEKLVQEYPNIKARIIFNATTEEKDFRKDVVAHLLAVEAG
                     RDAEKTKAALDTWYGMPQKKLSILAAAYPVNGLAEAQHEKIVAMDTWCRKVGIEYTPT
                     FFINGNQLPGAYSLGDIGYFLAE"
     gene            3316..>3527
                     /locus_tag="PK977_10970"
     CDS             3316..>3527
                     /locus_tag="PK977_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18686.1"
                     /translation="MQTKKMRLDAMQGKLSRAEMKNINGGVTNGGGCMQEFEYDCHMI
                     RTPFPCCEGLVCADNATNTGTICVSAS"
     assembly_gap    3528..3547
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3617..>3907
                     /locus_tag="PK977_10975"
     CDS             3617..>3907
                     /locus_tag="PK977_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HRF18687.1"
                     /translation="MYRDFIQLNCCILFFGLSLFSQQTEGKFFLKGKIEGVDNRLIYL
                     SYYDDNSSIGRLDSSIIKSGYFSFTGKINQPAVAFIKLNRKEAIGLNATNIFL"
     assembly_gap    3908..3934
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3935..>4747
                     /locus_tag="PK977_10980"
     CDS             <3935..>4747
                     /locus_tag="PK977_10980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF18688.1"
                     /translation="DLKLNDFRNAHLTGSNTQTDYEELRCKGDKIIKNHPLLFDSIGY
                     VQKNLDQSLYEKLSIVNDSLDMIDYEYFDQHPKSYLTAYLLQTHARKLSPDSLAIFYN
                     RLNDNLKQHPVTQTIRTRIINSKAGSVGSVAPSFIAKDTSGNDFYFSLLKGEYILLDF
                     WASWCVPCRENNPYLIELFNKYKSKGLKIVGIADERNLSQWKEAIKKEKIYIWIHVRR
                     QADIALKSMGLTDPGDINEQFNVSLLPAYILIDKYGVIIDRFESNEDSIKKL"
     assembly_gap    4748..4766
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4946..5194
                     /locus_tag="PK977_10985"
     CDS             4946..5194
                     /locus_tag="PK977_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18689.1"
                     /translation="MKKKISNLENKLSRKQMKQLSGGLFEGGEIGIELCAGPGNSVQR
                     CVCVGSTAVFYCCYNTLQACVTENGCGNSYVCSKNTSA"
     gene            5170..5667
                     /locus_tag="PK977_10990"
     CDS             5170..5667
                     /locus_tag="PK977_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18690.1"
                     /translation="MFKKYIRLIMIRSWLLFFGTCALFQFFLITPSSGQNQNCPQFSS
                     DLAKYYDSLANNIDSNIILEEKNITGRSLVLGIYSGRVLTTLDKFFLIEIKRSFPVVD
                     TIVLYVEGTSIFLLKYQHQTIYINNNVSYDKNRFECIKSEEILELIKELTLIVKGLNT
                     LIDNK"
     gene            5776..>7010
                     /locus_tag="PK977_10995"
     CDS             5776..>7010
                     /locus_tag="PK977_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HRF18691.1"
                     /translation="MDCGPTCLRMIAGYYGKKVSLDQVRRIAQLGKAGVSLLGLSEAA
                     EALGFKTVGAKISFNQLVQDAPLPAILHWGQAHFVVVHPRSFSKKNSIEIADPASGMM
                     RLSKETFLQKWAPFTENGETKGVVLLLEPAPGFNETSFAEDESEETPAIGWRYFSSFF
                     RYNRAYLFQVILGLVIASLLQLLFPFLTQSIVDKGINNRDIGFIQLVLLAQLMLLFSL
                     TVVEFIRSRLLLHISTRLNFSLLSGFWSKLLRLPMPFFDSRHPGDIMQRIGDHQRIEA
                     FLTGTAVNTLFSVINLLVFSFVLLRYNNTIFFIFAGGSLLYLLWIRLFLRLRRQLDYK
                     RFVIAGKENNATVQLVYGIQEIKLNNAENHFRWAWEGLQAGLFKLKFKSLALSQYQQA
                     GAFFINQGKNLLITFVVAR"
     assembly_gap    7011..7021
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7022..>7405
                     /locus_tag="PK977_11000"
     CDS             <7022..>7405
                     /locus_tag="PK977_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF18692.1"
                     /translation="DGQLSLGMMLAIQYIIGQMNSPLEQPIGFTQQAQDAKISLERLN
                     DVHTVEEEAPATSTSTGGLVCLPADGTIDLQNVSFTYPGAGNEPVLKNISLQIPEGKV
                     TAIVGISGSGKTTLLKLLLRFYENYT"
     assembly_gap    7406..7415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7416..>7912
                     /locus_tag="PK977_11005"
     CDS             <7416..>7912
                     /locus_tag="PK977_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF18693.1"
                     /translation="EIKLGPAKFSAIHPRYWRSVCGAVMQDPYIFNDNFRKNITVTDD
                     PVDEEKLYSACRTSNILSFIESLPLGFYTKLGAEGTAISGGQKQRLAIARAVYKSPHY
                     LFLDEATNSLDANNEKMILENLQTFFTGRTVVVVAHRLSTVMNADKIIVLENGRVAEE
                     GTHQQL"
BASE COUNT         2367 a         1424 c         1679 g         2355 t
ORIGIN      
        1 gggtttcagc atcggcatgc aatttcataa ataatgttca acaaataggg atttagcctg
       61 tggctcttag ctaactttgc agccgcaatg ggaaatgaat tagtacagcg ttcttccagg
      121 ccaatagcca tctggctgct ggtgggtgtg ggtatgatca ttattcagat tttattgggt
      181 ggaatcaccc gactcaccgg ttccggactt tctattacag agtggaatgt tgtaaccggt
      241 acactgcccc cgatgagtga gcaggaatgg ttgacagagt tcaataaata caaagcaaca
      301 ccacagttca acctgctgaa ctttgatttt accttaaaag atttcaaatt tattttcttc
      361 tgggaatggt ttcaccgttt ctgggcccgg ctgatcggcg tggttttctt tatacccttt
      421 gtcattttcc tggtgcaaaa gaggttcagt ccatctatgg tgaagccgat gatcattttg
      481 tttttactgg gggctttgca gggtgcggtg ggctggatca tggtagtcag cggcctaacc
      541 ggtgatgcca tctatgtaaa acccacccgg ctggctctgc atttcatttt tgcattgggt
      601 ttattggcct atactttttg gtttgcaatg gaactgctgg tgccaaaaaa agaactggtg
      661 atcaataaaa aagcaaggca actcaatatt gccattatag ttgttttggt cattcaatta
      721 atttatggcg ctttgatggc agggaataaa gccgctacgg cagcgccaac ctggccggat
      781 atcaatgggt atgcatttcc gcccaatatg atggggcatg gcgagggatt gttgaacttg
      841 ttggataata agatcactat tcattttata caccgggggc ttgcttacct tcttttaata
      901 atggttttgg tgcttacgta tcgtttgtac aaaacaggcg caacttcttt cctaaaaaaa
      961 ttgcgatggt ggccggcttt attggttatc atccaggtgg tgctgggaat attatctgtc
     1021 ctgaccagtt tacaaatcgt tcctaaccaa tggggtgttt ttgaatggat ggcacaacta
     1081 caccagttag tggctatgtt ccttctgttg tcattgattg ctttgcttta tagcataaag
     1141 agtaaataga atttaatgta gattcaaaaa gtatttaata ctccaaaaat tactcaagaa
     1201 tttcgggggg agtaaagcat ccaaaaactt aatggctgta tctgcagtat atcccaatac
     1261 tttcaatatt tcctctatcc tgtttgcttg aaaccgtgga tggttttcca actccgagta
     1321 ccgctgtttg gtaatgccca gtttacgggc cacctactct tgtttcattt gacgtgacaa
     1381 acgtaaaagc ctgatttgtt catttgtaaa ctggtgcata aatcgtaagg gtcattgatt
     1441 ttaataaaat tatatatact gccttgtatt tccatgtatt atatacaaaa taccactttc
     1501 gtggtattga ctttgtcaag gctgccaaat ttggatgatt atttaactag tccgaaattc
     1561 agttatccta aaaaattttg tattcttttt tgatgtggtt tagttacgca ccagaagtaa
     1621 aggcagtaat acgctttata gaattactga aagtaagagt tacccgttct actatcagcg
     1681 aaacactgca agcccactcc gattggccaa gtttgctttg cattagcgac agtctgaagc
     1741 aatggcatat acccaatgcc gctgctaaaa tagataatgc agatttgaat cagttgcccg
     1801 ttccctttat agcatatctg cccggccaac ccgtacccat tactgtagta actgaaatag
     1861 cagataatac gattacgact tacaatgata attttacccg aagagtggcc ctgcctaaag
     1921 aaaaattttt aagtctttgg acgggcattt atcttctggc tgagcctaca gttaaaagcg
     1981 gcgaaaaaaa ctaccttcaa aaaaagaaaa gagcatttgt tgcctcactg acgccggtat
     2041 tattgctgat attgataata ggatgctgta tcttttggct tgataagaat ttacaaataa
     2101 tcaatctttc acaacaggcc ggtgtatggt ttcaatatgg agtagtattg accggggtag
     2161 cggtatgtat attattgctg tggtacgaaa tggaccagaa caacccgctg ttaaataaag
     2221 tatgcaccgg tgtagcccgt ggtaactgca gcgccatact tacgggtaag gcatcgaggc
     2281 tatttagctg gcttagctgg agcgaagtgg gttttttctt ttttgcagga agcctgttct
     2341 tgatagtgct gaccccttat aaccctgtac catatgcttt aaaccttgtt gcgctacctt
     2401 atattttctt ttcggtgtat taccaatggc aggtggcaaa gcagtggtgt gtgctgtgcc
     2461 tcgctgtgca ggtattactt ctggccgggg cggtaaatgt aatgatccat tttttgccct
     2521 tgttagggtt gtttactaca aaaaatattt tacaaagttt tgctgcatac ctgctaatgc
     2581 ccttgttgtg gtattctata aagccgctgt ttcttaattt acaggggcta aaaggcgaca
     2641 agcggcaata cctgcggatg aagtttaata cagaagtatt tgaaacattg ctggtcaggc
     2701 aaaagaaaat tactgctccg acagcagact tgggtatagt tataggcaat ccccatgcac
     2761 agcatgaaat agtaaaagtg tgcaatccct attgtgggcc ctgtgctgca tctcacccta
     2821 aattagaaaa gctggtacag gagtacccaa atattaaggc aaggataatt tttaatgcca
     2881 caacagagga gaaggacttt agaaaagatg tggtggccca cctgcttgcc gtggaagcag
     2941 ggcgagatgc cgaaaaaaca aaagcggcgc tggatacatg gtatggcatg ccacaaaaaa
     3001 agctgagtat attggctgct gcttacccgg taaacgggct ggctgaagca cagcacgaaa
     3061 aaattgtggc catggataca tggtgccgca aggtgggtat agaatatacc cccacctttt
     3121 ttataaacgg aaatcaattg cccggggcct acagcctcgg ggatatagga tattttcttg
     3181 cagaatagtt ccctgtaaga aaagccccgg cggggctaat cccgcatgcg caggcgggaa
     3241 gccgggcggc caatgccttt gatgccgaaa gctaaggcaa caaaccattt ttttaaaact
     3301 ttaaatacaa aaacaatgca aacaaaaaaa atgaggcttg atgccatgca gggaaaattg
     3361 agcagggcgg aaatgaaaaa tatcaacgga ggagtgacca atggaggagg ctgcatgcaa
     3421 gaatttgagt atgattgtca tatgataaga acaccctttc catgttgtga aggactagtt
     3481 tgtgctgaca atgcaacaaa cacaggaact atttgtgttt ctgcctcnnn nnnnnnnnnn
     3541 nnnnnnntat tgataagcgg aaagcttcag atatctaata gatcagctgc cggaatcaat
     3601 cgaaatagtc aatctgatgt acagagattt tatacagtta aattgttgca ttttattttt
     3661 tggtcttagt ttgttttcac aacaaacaga gggaaagttt tttttaaaag gtaaaataga
     3721 aggagttgat aacaggttga tttatttgag ctattatgat gataattcta gtatagggag
     3781 gctggatagt tcgataatta aaagcggtta tttttccttt accggaaaaa ttaaccagcc
     3841 ggcagttgca tttattaaac taaatagaaa agaagctatt ggtttaaatg caactaatat
     3901 ttttttannn nnnnnnnnnn nnnnnnnnnn nnnnttgatc taaagctgaa cgattttcgc
     3961 aatgctcatc tcacaggttc caatacacaa acggattatg aagaattgag gtgtaaaggg
     4021 gataaaatta ttaaaaatca tcccttgctt tttgatagca ttggatatgt tcaaaaaaac
     4081 ttggatcaat ccctatatga gaaactttct attgtaaacg actcccttga catgattgat
     4141 tacgaatatt ttgaccaaca tccaaaatcg tatttaactg catacttatt acaaacccat
     4201 gcaagaaaat tatcaccaga ttcattggca atattttata accgattaaa cgataaccta
     4261 aaacaacatc ccgttaccca gactattcgc acaagaataa taaactctaa ggctggttcc
     4321 gtaggatcag tagcaccaag ttttatagct aaagacacca gcgggaacga tttttatttt
     4381 agcttattaa agggggaata cattttactt gatttctggg caagttggtg tgttccatgc
     4441 agggaaaata atccttactt aattgagcta tttaataaat ataaaagcaa aggactaaaa
     4501 atagttggga ttgcagatga aaggaacctg tctcagtgga aggaagcaat caaaaaagaa
     4561 aaaatatata tatggataca tgttcgcagg caggcagata ttgcgttaaa aagtatggga
     4621 ttgactgatc ctggtgatat taatgagcag tttaatgttt cattattacc tgcttatatt
     4681 ttaattgaca agtatggagt aattattgac aggtttgaat caaacgaaga ttctattaaa
     4741 aaactacnnn nnnnnnnnnn nnnnnnggaa ctcttgttta aagaaaaata gtgtgtataa
     4801 ataaatattt tcttgcagaa cagctccctg taagaaaggc ccggcggagc caagtggcga
     4861 agtacagccg cagctgggcg gccaatgcct ttgatgccaa aatctaaagc aacaaaccaa
     4921 tttttttaaa ctaaaccaga atgaaatgaa aaaaaaaatt agcaatcttg aaaacaaatt
     4981 aagtagaaag cagatgaaac agttatctgg cggtcttttt gaaggtggtg aaatcggaat
     5041 cgaattgtgt gctggccccg gtaatagtgt gcagagatgt gtttgtgtag gcagtactgc
     5101 ggttttttat tgctgctaca acactcttca ggcatgtgta actgaaaatg gatgtggaaa
     5161 cagttatgta tgttcaaaaa atacatccgc ctgataatga ttagatcatg gttattattt
     5221 tttggaactt gtgccctttt tcaatttttt ttaataacac caagttcagg gcaaaatcaa
     5281 aactgcccgc aattttcaag tgatcttgca aagtattatg attctttggc caataatatt
     5341 gacagcaata ttattttgga agaaaaaaac attacaggaa gatccctggt gcttggcata
     5401 tattcaggca gagttttaac cactttagat aagttttttc ttatcgaaat aaaacgatcg
     5461 ttcccggtcg ttgatacaat agttctttat gtagagggga ctagtatttt tttattgaag
     5521 tatcaacatc aaacaattta catcaataat aatgtgagct atgataaaaa taggtttgaa
     5581 tgtataaaat cagaagaaat attagaattg atcaaggaat tgactttgat tgtaaaggga
     5641 ttaaatacat taattgataa taaataaaat cctaaagtga tacacataag gagataaaac
     5701 agatttttcc ttctttattt attcgttaat tctacattta atttgaagtt ccggtttcat
     5761 aaacaaccta acgctatgga ctgtggcccc acctgcctgc gcatgatagc tggctattat
     5821 ggcaagaagg taagtttaga tcaggtgcgc cggattgcac agttaggcaa agcaggcgta
     5881 agcttattgg gtttatcaga ggcagcagaa gcactcgggt ttaaaacagt aggagcaaaa
     5941 atttctttta atcagttagt acaggatgcg ccacttcctg cgatactgca ttggggacag
     6001 gcacattttg tagtggtaca tccccgctcc ttctctaaga aaaattcaat tgaaattgcc
     6061 gatccggcaa gtggcatgat gcgcctttcc aaagaaacat ttttgcaaaa atgggcacct
     6121 tttactgaaa atggagaaac aaagggggtc gtcctacttt tagaacctgc acccggtttt
     6181 aatgaaactt catttgcaga agatgaaagt gaagaaacac cagcaatcgg gtggcgttat
     6241 ttttcttctt tttttaggta caacagggct tatcttttcc aggttatttt gggtttagtt
     6301 attgcaagtc ttctgcaact gctttttcca tttcttaccc aaagtatagt ggataaagga
     6361 attaataaca gggacattgg ttttatacaa ttagtgcttc ttgcacaact aatgctgctt
     6421 ttttcattaa ccgtggtgga gtttatacgc agtcgtttgt tactgcatat cagtacaagg
     6481 cttaattttt ccttgctatc tggtttttgg tcaaagttgc ttcgcctgcc tatgccgttt
     6541 ttcgacagcc gacacccggg tgatattatg caacgcattg gcgaccatca gcgtatcgaa
     6601 gcctttctta ccggtactgc tgttaatacg ttattctctg ttattaattt gcttgttttc
     6661 agttttgtat tacttcgtta caacaatact atatttttca tatttgccgg gggcagcttg
     6721 ttatacctgc tttggataag attgttttta aggctccgtc gtcagctgga ttataaacgg
     6781 tttgtcattg caggtaaaga aaacaacgct actgttcaat tggtatatgg catacaggaa
     6841 ataaaactaa acaatgccga aaatcatttt cgctgggcat gggaaggttt gcaggcaggg
     6901 ctttttaaat taaagtttaa atcattggcc ttaagccagt accagcaggc cggggccttt
     6961 tttataaatc agggtaaaaa cctgctcatc acttttgtag tggccaggtt nnnnnnnnnn
     7021 nagatggcca gttgagcctt ggaatgatgc tggccattca atatattatt ggacagatga
     7081 acagcccctt agaacagccg atagggttta cacaacaggc gcaggatgct aaaatatctt
     7141 tggagcgatt gaatgatgtg catacagtgg aggaagaagc tccggctact tccacttcaa
     7201 ctggaggatt ggtttgcttg cctgctgatg gtactattga tttgcaaaat gtatctttca
     7261 cctatccagg tgcgggcaat gagccagttt tgaaaaatat tagtttacaa atacccgaag
     7321 gaaaagttac agccatagtt ggcatcagtg gtagcggaaa aacgacattg ctgaaactgt
     7381 tgctacgctt ttacgaaaac tatacnnnnn nnnnngaaat aaaattaggc cccgctaaat
     7441 ttagtgctat ccatccaagg tactggcgaa gtgtatgcgg tgctgtgatg caagacccct
     7501 atatctttaa tgataacttc cgaaaaaaca taacggtaac cgatgatccg gtggatgaag
     7561 agaaactata tagcgcctgc cgcaccagta atatactttc atttattgaa tcattaccgc
     7621 ttggctttta tacaaaacta ggagccgaag gtacggccat cagcggcgga caaaaacaac
     7681 ggcttgccat tgcaagagca gtatataaaa gcccacacta tcttttctta gatgaagcca
     7741 ctaactcttt agatgccaat aatgagaaaa tgatattgga aaacctgcaa acatttttta
     7801 ccggccggac agtagtagtt gtagcacacc ggctcagtac agttatgaat gcggacaaaa
     7861 taattgtgct cgaaaacgga agggtagccg aagaaggtac ccatcagcaa ct
//
LOCUS       DAORLS010000136         7878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_4987_length_7878_cov_1.898632, whole genome shotgun sequence.
ACCESSION   DAORLS010000136 DAORLS010000000
VERSION     DAORLS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_7878_cov_1.898632"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PK977_11010"
     CDS             <1..574
                     /locus_tag="PK977_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18694.1"
                     /translation="NVPVTNGTFSLTVLRCSSGTVNFTVLGVDYTSIQQSVPVSGSGT
                     TGTINVGTVQACGTSSAQFLELIIDGTPYNYVSPPDNFNYSDSTIAGVYTNKTWIFAF
                     RQNAGTTTYSSFSFNNNAAAATGLPLNNLNVYLGAALNSQTVTTASPVVNVTGFGPVG
                     TGFVEGNFNVQMDFAGTIRNVVCSFRVRRM"
     gene            779..>1315
                     /locus_tag="PK977_11015"
     CDS             779..>1315
                     /locus_tag="PK977_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18695.1"
                     /translation="MKRIFKMTALVLAIVLGSSIRQTASAQYYDDRGEVTYQTFYDEL
                     SPHGRWIEYPEYGYVWVPDADPDFRPYSTNGHWVWTDDYEWMWVSDYDWGWAPFHYGR
                     WLEDDYYGWVWVPGYEWSPAWVAWRDGGDYYGWAPLRPGINISINFNIGSYSPPYSYW
                     NFAPRRYLAYRNIGSYCID"
     assembly_gap    1316..1368
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1369..2140
                     /locus_tag="PK977_11020"
     CDS             <1369..2140
                     /locus_tag="PK977_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18696.1"
                     /translation="SYNRNVFRTGPRRYDAERYIGRINPVRFRDTYTPGRTFYRNNEV
                     SVYRPNVRRDDNRNFTPRQFDRYDKRDAANRNGRSDNGFRRNENGGRNDITERRNNNL
                     PQRREFERRDDNNVRQPQNNYDRRRFDRNDNNSRIPDPDDRADNRRVNRNMERPDRNV
                     YQNPGNNGNDRRFERPNNDNRNYNPPSREPRVRENNNGGYQRPAPQQMERRQETRQQP
                     QQQPGRQFERRDNGNRGGGQPNGGNNGNNGSGRGRSRF"
     gene            complement(2218..>3933)
                     /gene="gcvP"
                     /locus_tag="PK977_11025"
     CDS             complement(2218..>3933)
                     /gene="gcvP"
                     /locus_tag="PK977_11025"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRF18697.1"
                     /translation="ENFFDSLTVKVPDINVIRQKVEKQQINLRYISNDLIGISLDETT
                     EVADLFDLINCFENDTDPVAFDIPQDAELNRLPAGLVRTSAYLTHPVFNTYHSESQMM
                     RYIKHLENKDLSLNTSMISLGSCNMKLNAASEMMPLSWNHWFRMHPFAPASQVEGYKF
                     IIDELSKYLCEITGFDACSLQPNSGAQGEYAGLLTIRNYHEANGDHHRKVMLIPISAH
                     GTNPASAVMAGMKVVVVKALDNGYIDVVDLKTKAAQYSNELAGIMITYPSTYGVYEET
                     VKEITEIIHLHGGQVYMDGANMNAQVGLTAPGLIGADVCHLNLHKTFAIPHGGGGPGM
                     GPICVKAHLAKHLPGHVTGGTSNAVSAAPFGSASILLISYGYIRMLGPDGLKSTTEYA
                     ILNANYMRARLQGAYDILYLGTNGTCAHEFIVDLRPFKKSAEVEAEDVAKRLIDYGFH
                     APTMSFPVPGTIMIEPTESEDKAELDRFCDALLSIREEIKEIEEARADKKDNPLKNSP
                     HTQLVVTADEWKHSYTRQTAAFPLYYITQNKFWPSVARVNNTHGDRNLICTCEPVEAY
                     MEAEA"
     assembly_gap    3934..3967
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3968..5095)
                     /locus_tag="PK977_11030"
     CDS             complement(<3968..5095)
                     /locus_tag="PK977_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRF18698.1"
                     /translation="MTLFEQQANEFATRHIGPNEHFTRQMLRTIGVKSMDELIEKTVP
                     ASIRMEKALNVPAAMTENEYLKHIKEVSLKNKVFTNYIGQGYYDTITPSVILRNIFEN
                     PGWYTQYTPYQAEISQGRLESLLNFQTMVSDLTGLPIANASLLDEATAAAEAMTMFFN
                     TLNKQDHIERPKFFVDKEIFPQTKDVLLTRAVPVGIEIIEGDYKTATINDSFFGAIVQ
                     YPNDKGSIEDYQDFISTVHGAGAFIVMATDLLALTLLTPPGELGADVAVGSAQRFGVP
                     LGYGGPHAAFFAAKDDFKRSMPGRIIGISIDAQGNRALRMALQTREQHIKREKATSNI
                     CTAQALLANMAAMYAVYHGPTGLKNIAKRIALLTQTVADAIE"
     gene            5295..5780
                     /locus_tag="PK977_11035"
     CDS             5295..5780
                     /locus_tag="PK977_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRF18699.1"
                     /translation="MQKDILNNWQKKSGERQKLYKQFLQRAKKNDVLRQLPDLHDEAV
                     SKIDCLQCANCCKNYSPRFKTPDVKRISKHLGMRESEFIDVYLKVDEEGDFVAKTLPC
                     PFLGADNYCSIYDQRPSDCQRFPYTDEDVVIKRPALTLKNSTFCPITYYVLEGLLAEK
                     K"
     gene            5839..6354
                     /locus_tag="PK977_11040"
     CDS             5839..6354
                     /locus_tag="PK977_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRF18700.1"
                     /translation="MKKSGTIKKSREYTLKQEEITSMAEEPAVKYKSLNVILGGKQSI
                     TQPVSGDFDLIALTREGIKKSSLKSLAAHLGVTMEVMSRLLHSSHRNMQRKDEDELLD
                     TLKTEKVLELAAFAQRGIDVVGSKQAFIEWLHSPLVSLDNKSPLEFLDTSFGIQLLQK
                     ILGRLEQGVYS"
     gene            6384..6851
                     /locus_tag="PK977_11045"
     CDS             6384..6851
                     /locus_tag="PK977_11045"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRF18701.1"
                     /translation="MELYRIAQEKYAEDLSGNGARLFGGRWNSEGQFALYTSSTRSLA
                     LLETLAHAPAKLLQAKTYQLITLFVPDTAAIQTLEAKKLPHGWGDVEISPFTQKTGDQ
                     FLMAKKNLVLAVPSVLIPEELNYVLNPLHTDFKKVKILNKRRILFDKRVQGNL"
     gene            complement(6854..7750)
                     /locus_tag="PK977_11050"
     CDS             complement(6854..7750)
                     /locus_tag="PK977_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF18702.1"
                     /translation="MRRRQFIQNTAFAFGALAIADQKILKALFEDPWKIKMLTDKVGI
                     FTERGGTIAFFLSDEGIIIVDSQFPDQSKHLIDELKKRSEKPFRLLINTHHHGDHSGG
                     NISFKGLVENVLAHNNSLINQEKSAKQNKAEDKQLYPDLTYNYTWARTFGKEKVRLHY
                     FGPGHTNGDSFIHFEDANIVHCGDLVFNRRHPYVDRGAGANMKSWINVLDEALNKFDS
                     KTTFVYGHAGTGYEITGKADDLRAFSDYLGNVLKYVEGEIKAGKTKEEILKSTAIPGA
                     DQWKGDGIQRPLTAAWEELTTK"
BASE COUNT         2217 a         1604 c         1677 g         2293 t
ORIGIN      
        1 caatgtgccg gtaacgaatg gcacattcag ccttactgtt ctgcgatgca gcagtggcac
       61 tgtgaatttt accgtacttg gtgtagatta tacttctatt cagcaaagtg ttcccgtaag
      121 cggaagcggc acaaccggta ctattaatgt cggaactgtt caggcatgtg gtacctcatc
      181 agcccagttt ctggaattaa ttattgacgg cacaccttat aactatgttt ccccgcctga
      241 taattttaat tattcagact ctacaatagc cggtgtttac actaataaaa cctggatatt
      301 cgctttcaga caaaatgctg gtacaacgac atatagttcc ttttcattta ataacaatgc
      361 agccgctgct accggattac cacttaacaa cctaaatgtt tatttgggag ctgctttgaa
      421 ttcccagaca gttactacag caagccccgt agttaatgtt accgggtttg gccctgtagg
      481 cactggtttt gttgagggta atttcaatgt acaaatggac tttgccggta ctatccggaa
      541 tgtggtctgt agtttcaggg ttcgtcgaat gtgacaaatt caatattttt ttacaagggg
      601 tgtgctaaat gcacaccttt tttattgtta acagtaatac aatgttttgc taaaacaaca
      661 tcctgtattt tccagggcaa ccatcatggc atagttatag cgtcatatgg atgtaataaa
      721 gaagaaaacc acttaacaat tttccgtatg catcaattta ttacaaaaaa gtatgtccat
      781 gaaacgcatc tttaaaatga ctgcattggt actggcgata gtactgggca gttctattcg
      841 acaaaccgct tccgcccagt attacgatga cagaggcgaa gtaacttacc agacctttta
      901 tgatgaatta agccctcatg ggcgatggat cgagtacccg gagtacggct atgtttgggt
      961 gcccgatgcc gatcctgatt ttcgtcctta tagtaccaac ggtcattggg tatggacgga
     1021 tgattatgaa tggatgtggg tgtcagatta tgattgggga tgggcgccat tccattacgg
     1081 ccggtggttg gaggatgatt attacggctg ggtatgggta cccggttatg aatggtctcc
     1141 ggcctgggta gcctggaggg atggtggcga ctattatggc tgggcaccat tgcgcccggg
     1201 aatcaacatt tccataaact tcaacatcgg cagctattca ccaccttata gttactggaa
     1261 ttttgctcct agacgttatt tggcatacag aaatatcggg tcttactgta ttgatnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gttacaatcg
     1381 taacgttttc cgcacaggcc cccgcaggta tgatgctgaa aggtatattg gcagaattaa
     1441 ccctgttaga ttccgtgaca cttatactcc tggcaggacg ttttacagaa ataacgaagt
     1501 aagtgtttat cgtccaaatg ttcggagaga tgataaccgg aactttaccc caaggcagtt
     1561 cgatcgctac gacaaaagag atgctgccaa cagaaatggc aggtctgata atgggttccg
     1621 caggaatgaa aacggtggca gaaacgacat tacagaacgt cgaaacaata atctgccaca
     1681 gcgccgtgaa tttgaaagaa gagacgataa taatgtaaga caaccccaga acaattatga
     1741 ccggagaaga tttgacagaa atgataataa ttcccgcata cctgatcctg atgacagagc
     1801 cgataacagg agggtaaaca gaaatatgga acggccagat agaaacgttt atcaaaaccc
     1861 gggtaataat ggtaatgata gaagatttga aaggcctaac aatgataaca gaaattataa
     1921 tccaccttca agagaaccaa gagtgagaga aaataataat ggaggttatc agcgacctgc
     1981 tccacaacaa atggaaagaa gacaggagac caggcagcag cctcagcaac agccgggtcg
     2041 ccagtttgag agaagagata atggcaaccg gggtggaggt cagcctaatg gaggaaataa
     2101 cggaaataat ggaagtggga ggggccggtc aagattttaa ttttaaaatt agtccttgtg
     2161 taaatattaa aggccgttta atacaaacgg cctttagtat tttattagat ctaaacttta
     2221 tgcctctgct tccatatacg cctcaaccgg ttcacaagta caaatcaagt ttctgtcacc
     2281 atgggtatta tttactcttg ctacagaggg ccagaattta ttttgggtta tatagtaaag
     2341 agggaatgca gctgtttgtc ttgtataact atgtttccat tcatctgcag taactaccaa
     2401 ttgtgtatgt ggtgaatttt tcaggggatt atcttttttg tcagctcttg cctcctcgat
     2461 ttcttttatt tcttcccgta tagataataa ggcatcgcag aatctgtcca gttctgcctt
     2521 gtcttcactt tctgttggtt caatcataat agttccggga acaggaaaac tcattgtggg
     2581 tgcatgaaag ccatagtcaa tcagtcgttt tgccacatct tcagcttcta cttccgctga
     2641 ttttttgaat ggcctcagat caacgatgaa ttcatgagca caggtaccat ttgtcccgag
     2701 gtaaagaata tcgtaagctc cttgcaatct tgcccgcata tagttggcat tcaggatagc
     2761 atattcagta gttgacttca aaccatcagg ccctaacata cggatgtatc cgtagctgat
     2821 aagcaggata gatgcagaac caaaaggtgc tgctgatact gcattgctgg ttcctcctgt
     2881 cacatggcct ggcaaatgtt tcgccagatg tgcctttaca caaataggac ccatacccgg
     2941 accaccacca ccatgcggaa tagcgaatgt tttatgcagg ttcaggtggc atacatccgc
     3001 accaattaaa ccgggagcag tcaggcctac ctgggcgttc atattggctc catccatgta
     3061 aacctgtccg ccatgcagat gaataatttc agtgatctct tttaccgttt cttcatacac
     3121 tccataggta ctgggatatg tgatcataat gcctgccagt tcgttgctgt attgagcagc
     3181 tttcgttttc aggtctacaa catcaatgta tccgttatcc aatgctttca ctactactac
     3241 tttcatacca gccattacag ctgaggcagg gttggtgcca tgtgctgaaa tgggtatcag
     3301 cattacttta cgatgatgat caccattggc ctcatggtaa ttacgaatgg ttaaaagacc
     3361 tgcatattct ccctgtgcgc cactattggg ctgaaggcta caggcatcaa atccagtgat
     3421 ctcgcagaga tatttgctta attcatcaat gatgaattta tatccttcaa cctgactagc
     3481 aggggcaaaa ggatgcattc tgaaccagtg attccagctt agcggcatca tttcagacgc
     3541 tgcattcagt ttcatgttac agctgcccag tgaaatcatt gaagtattta aggaaagatc
     3601 tttattttcc aggtgcttga tatacctcat catctggctt tcactgtgat aggtattgaa
     3661 aactggatgt gttaggtaag ctgaagttct gacaagacct gccggcaggc ggtttagttc
     3721 agcgtcctgt ggaatatcaa aagcaacagg gtctgtgtca ttttcaaaac aattgatcag
     3781 gtcaaaaaga tcggctacct ctgtggtttc atccagggag attccaatca ggtcattgct
     3841 tatatagcgt agattgattt gttgtttttc tactttttgc ctgataacat taatatcagg
     3901 cacttttacg gtaaggctat caaagaaatt ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnttc aatggcgtcg gccacggttt gagtcaacaa tgctattctc tttgcaatgt
     4021 ttttaagacc ggtagggcca tgatagaccg catacatggc agccatgttt gccagcaaag
     4081 cctgcgcagt acaaatattt gaagtcgcct tctccctttt gatatgctgc tctcttgttt
     4141 gcagcgccat tcttaaggcc cggttacctt gtgcatcaat gctaattccg atgattctgc
     4201 ccggcatgct tctcttaaaa tcatcttttg cagcaaagaa agctgcatgt ggtccaccat
     4261 atccaagcgg tacaccaaag cgttgtgcag aaccaacagc aacgtctgcc ccaagttcac
     4321 caggcggtgt gagtaatgtt aatgcgagca ggtcagttgc cattacaata aaggcccctg
     4381 ctccgtgaac tgtacttata aaatcctggt aatcttcaat agatcccttg tcattgggat
     4441 attgaacaat tgcaccaaaa aagctatcgt ttatggttgc tgttttataa tctccctcta
     4501 ttatttctat tcctacaggt acggctcttg tcagtaatac atcctttgtt tgaggaaata
     4561 tttccttatc aacaaaaaat ttaggtcttt ctatgtggtc ttgtttgttc aacgtattaa
     4621 aaaacatggt cattgcctcg gcagcagcag tagcttcatc gagcagggag gcatttgcta
     4681 tcggcaatcc ggtaagatcg ctgaccattg tctgaaaatt caacaggctt tccagtcgtc
     4741 cctgtgatat ctctgcctgg tagggtgtat actgtgtata ccaacccgga ttttcgaata
     4801 tgtttcgaag gatcacagat ggagtaattg tatcatagta tccttgtccg atatagttcg
     4861 taaagacttt atttttcagg gagacttcct ttatatgctt taggtattca ttttctgtca
     4921 tcgcagcagg aacattcaat gctttttcca tccggattga agcaggtacg gttttttcga
     4981 taagctcatc catactcttt acaccaatag ttcttaacat ctggcgggta aaatgttcat
     5041 tgggaccgat gtggcgggtg gcaaattcgt tagcctgttg ttcaaagaga gtcataaaag
     5101 gtaatgtttt ttatagggtt gatggtacat gaacttttgg ggaatcatgg ccgatcaaca
     5161 aaatcttctt ttgcgcagca aagttacggg gtggtattca gaaaacaggt gagttgttga
     5221 ggatggtgga taataaatag ccgatttggc caactgtcct gttattaagg ggagacgaaa
     5281 ttgtaaattg cgccatgcaa aaggatatac ttaacaattg gcaaaaaaaa tcaggtgagc
     5341 ggcaaaagtt gtacaagcaa tttttgcaaa gggctaaaaa gaatgatgta ctgcgtcagc
     5401 ttcctgacct gcatgatgaa gctgtttcaa aaatagattg ccttcagtgt gccaattgct
     5461 gtaaaaatta ttcccccagg tttaaaaccc ctgatgtcaa gcgcatcagc aaacaccttg
     5521 ggatgcggga gagtgagttt attgatgttt acctgaaagt ggatgaagaa ggagattttg
     5581 tagcaaaaac gcttccttgc ccctttctgg gagcagacaa ctattgctcc atttatgacc
     5641 agcgaccatc tgattgccag cggtttccct acacggatga agatgttgtg atcaaacgtc
     5701 ctgcacttac cttaaagaat tctacttttt gccctattac ctattatgta ctggagggtt
     5761 tattggcaga gaaaaaataa tacgacaaaa tggatattta ttttgcaaaa tgtcgtattt
     5821 ttggaaaaag cacaaaggat gaaaaaatcc ggtacaataa aaaaaagcag ggagtacact
     5881 ttaaaacaag aagaaatcac ttcaatggca gaagaaccag ctgttaagta taaatcattg
     5941 aatgtaattc tcggcggtaa acaatcaatc acccaacctg tttccggaga ttttgacctt
     6001 attgccctta ccagggaagg tatcaagaaa tcatcattaa aatcattggc cgctcacctg
     6061 ggggttacta tggaagtgat gagccgtttg ttgcatagtt cccaccgaaa tatgcaacga
     6121 aaggatgagg atgaattgct cgatacacta aaaaccgaaa aagtactgga actggctgcc
     6181 tttgcccagc ggggcataga tgttgtcgga tcaaaacaag cattcattga atggctccat
     6241 agcccccttg tttcccttga caataaaagc cctcttgaat ttcttgatac atcgtttggt
     6301 atacaattac tccaaaagat tctgggaaga ctagagcagg gagtttattc ataacaataa
     6361 ggtgggaata ttttacagca gtcatggaat tatatcggat agcacaggag aagtatgcag
     6421 aggatctgtc gggtaatggt gcccgtttgt tcgggggccg gtggaatagc gaaggacagt
     6481 ttgcccttta tacttccagt acccgttcac ttgccctcct ggaaactctg gcgcatgcac
     6541 cagctaaact tttgcaggct aagacttacc aactaattac tttgtttgta cctgatacag
     6601 cagcgataca gacgttagag gctaaaaaac tgcctcatgg atggggtgat gttgaaataa
     6661 gtccctttac tcaaaaaaca ggcgatcagt ttttaatggc aaaaaaaaac ctagtacttg
     6721 cagtacccag tgtgctaatt ccggaagaac tgaattatgt tttaaatccc ctgcatactg
     6781 attttaaaaa agtaaaaatc ttaaataaaa gaaggattct tttcgataaa agggtgcagg
     6841 gtaatttata atattacttt gttgtcaatt cttcccaggc agctgttaag ggcctttgta
     6901 ttccgtcacc tttccactga tcagcaccgg gaatagctgt ggattttaga atctcctctt
     6961 ttgttttccc ggcttttatc tcgccttcca catatttcaa tacgttgccc aggtaatcac
     7021 tgaatgcccg tagatcatcg gcttttccgg taatctcgta gccagttccg gcatgcccgt
     7081 aaacaaatgt cgtcttgcta tcgaatttat taagggcttc gtccagcaca tttatccagc
     7141 ttttcatatt agcacctgca cccctgtcca cataagggtg acggcggtta aatacaaggt
     7201 caccacagtg tacaatatta gcatcctcaa aatgaataaa gctatcgcca ttcgtatgtc
     7261 ccgggccaaa ataatgtaaa cggactttct ctttgccaaa tgttcttgcc caggtgtaat
     7321 tataagtcag gtcagggtat agctgtttgt cttctgcttt attttgttta gctgattttt
     7381 cctggttgat caggctattg ttatgcgcaa gcacattttc aacaagacct ttaaatgaga
     7441 tattaccgcc gctatgatcg ccgtgatgat gcgtattgat caaaagcctg aaaggttttt
     7501 cgcttctctt ttttagttca tcaatcaggt gcttgctctg atcagggaat tgcgaatcaa
     7561 ctattataat accttcatca gacaaaaaaa atgcaatagt tcctcctctt tcggtgaata
     7621 tgccaacctt gtcagtcagc atttttattt tccaaggatc ttcaaaaagt gcttttaaaa
     7681 ttttctgatc agctattgcc agtgctccaa aggcaaaagc agtattttga atgaactgtc
     7741 ggcggcgcat atcgtttaat tgaaagggtt gaaaatggag tagtctttca atactcgaat
     7801 ttacgtaatc ccggcgcttt aaaccaggta actattacta cgagcaaagt catacacccg
     7861 ccaaaaatca cagagggg
//
LOCUS       DAORLS010000137         7846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5026_length_7846_cov_2.232191, whole genome shotgun sequence.
ACCESSION   DAORLS010000137 DAORLS010000000
VERSION     DAORLS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5026_length_7846_cov_2.232191"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PK977_11055"
     CDS             complement(<1..865)
                     /locus_tag="PK977_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HRF18703.1"
                     /translation="MQLRPVATYDHIEPQVFKKEFYEPAIPVVIKNLSKDWPAYTKWN
                     WEYFKQLVGDKKVPLYNNVKSDAYTPINKADDYKTFGEYIDMISKGPAGWRIFLFNIF
                     DHAPQLINDFTWPEHLAKGFVKRYPMLFTGGAGSITHMHFDIDMSHIFHTQFGGRKRV
                     LMFPFKEQYKLYRKPWEVLSLADFSNYYQEGKVDYEKFPALKLAQGLDFILEPGDTLF
                     MPSGYWHHMEYLDSGFAMSLRAMQPSLAGKLKGLWNIVGMRNIDTVMKKTAPHWWYKR
                     KEKKIFEQAERE"
     assembly_gap    919..932
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            981..1592
                     /locus_tag="PK977_11060"
     CDS             981..1592
                     /locus_tag="PK977_11060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRF18704.1"
                     /translation="MILRIHPKDPQPRLIKQVVAKLQEGGIIIYPTDTVYGLGCDILQ
                     QKAVERICRIKKVEPRKAQLSFVCSDLSHLSDYAKPLSNPVYRLLKEHLPGPYTFILP
                     ASKMVPKIMQSKKDTIGLRIPDNRIAQAIIEELGRPILSASLPGEMVEDYTDPEVMFA
                     NFHNEVDFVIDGGIGGMIPSTVVDCTSDEPVVTRMGLGEWEEK"
     gene            complement(1738..3027)
                     /locus_tag="PK977_11065"
     CDS             complement(1738..3027)
                     /locus_tag="PK977_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18705.1"
                     /translation="MKKFIIYFLILVNSGFLQAQNVGIGTNSPSARLEGVGTTSSASG
                     NVLMLRNSNADTLLRMNNDGKMGIGYNGSYGRTLNIGGNGINFFDENNEFSGSIYPTN
                     SNALWIRSDDKMLILQPFAQNVGIGVTNPTSKLHVNGDIKGTGVIEGNGISSTGILFV
                     NQTSLLQGAVTGFSSAQFTGNIGSNTGITINNSSAILQMQSGGNNKGFVQLSGDNFRF
                     GTNSGNNNGNVVLRMDGTDMIEFQKTGSGGTFMQMNLNGTSTGVLQTTSTGNVSLTAV
                     NANAQVQLGGEVFINNTTNTTGIGTSSPGERLHVNGNMIVNGSAVIDDGRITGTATGS
                     AYNLLPVCYGRVNENGTKAGGTLNFTVSKTSLGSYEIVSSQITSSSIIMVNAWGANSW
                     CRGIGYYLSANTMRVSIVDILNTDPRDCAFHFIIYNP"
     gene            complement(3038..3328)
                     /locus_tag="PK977_11070"
     CDS             complement(3038..3328)
                     /locus_tag="PK977_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HRF18706.1"
                     /translation="MIAQKIDEIKDEMRKAGTWKKEMPEWVKDFEKRQIDNSDDFSEW
                     LQFVYLPNLLHTSRSVITTDERKFIAPQAIRYFGDDLKKGKLLQLLIELDSL"
     gene            3519..6509
                     /locus_tag="PK977_11075"
     CDS             3519..6509
                     /locus_tag="PK977_11075"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF18707.1"
                     /translation="MPALATIRIIICLLLLQQPVIAQQSGYRLQTLGTRDGLLSSKVY
                     ALKQASDRRLWIGTELGVSVYDGYGFDNFQYTITNETIGRVLCITEDAQKGIWVGGDK
                     GLFYSDGRNIRKVEKQGAVQLVVEALHTDENGNTWIGDISALYKITKEQSSVVKNNRG
                     KAINLSPVASFTQRVFSISSDRHQNIYIGANKGVFSIRPGADKYETVWENPDPARPVL
                     SVAAFSPDSVFWNCLNSHPAEMIRGKVKSTFTEDFIGRNVFKVHDKIFALTTSGVGVF
                     ANGIVKSLFSFGKITNNALAALVDAENNIWVGSWEGLQKFRKTGFRQYSLQHNDQKET
                     FSFLETKKGDLLFGSNRGLLFHKKEEAIVFNNGIAPLFPLAEVMCMYESEDESIWAGS
                     GYQGISRFKNGKLSNWNKTGWLKDNNCEALVPTPDGKLYACTENGVTLIDPAAAVPMV
                     AHYPFQKKLSRAPELFGGFHGGNSVYWFYGSRGLYCLKNGQLVNDSILGMPVTDLYIN
                     KIISDKKGNTWVATLGKGLLCCRQENGRLVLKKQYDNHNGLPSNNALSVLADKNDNIW
                     CGDYMSLSLLMHPGMNEHLLSFTEKDGLLESYYQTLKLEQQRNGTIWALTSMGVVSFH
                     PDSIILNDKAPVLLLNRIVSADGQYEHTSQHLSFKIGAVQIQYTAVSLTDPSATRYAY
                     RVTEQDSNWVYTNNRVADFSFLPPGTYTFELKASNNNNVWTTEPLRLNFTILPAFWQT
                     WWFMLVVVLIIAAFVYLFFRQRIRDIKRKAAIKQQVAELEAKAIRAQMNPHFIFNSLN
                     AIQESIVTEKVDVAYDYLSRFSKLLRMVLDNSEKTMIPLSSELETVRLYLSLEAMRFS
                     QSFTYTIEAGENLEKDDIPSLLLQPFAENAIWHGLINKEGEKKLLIRFEEKNDVLECV
                     IEDNGVGRTKAAAIKQQKLGSARFASKGTRLAMQRIEILNRERPGSASIETIDLLDEA
                     GNAAGTKVVIRLATEQLHKNEWRP"
     gene            6506..7255
                     /locus_tag="PK977_11080"
     CDS             6506..7255
                     /locus_tag="PK977_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF18708.1"
                     /translation="MIKILIIDDEQSAGRILKVLIEKHIPGEKQIEVSNDPAEALQIL
                     KEYQPTLVMLDIEMPGMNGFDFLNRASEWDFDVIFTTAYDKYAIKAIRFSALDYLLKP
                     VDIVELQNAVNRHIVKQQAPLKENRQLVNNLIYNLQQKDQQQFKLALSTSEGIFLYDP
                     KDIIALEGSSNYTRFFFAEHKPLIVAKTLKEYEDILTEHQFLRVHKSYLVNKLHVLKV
                     DRDGILEMSNQQHIPISRRRKAEILQWFGKK"
     gene            complement(7319..7624)
                     /locus_tag="PK977_11085"
     CDS             complement(7319..7624)
                     /locus_tag="PK977_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18709.1"
                     /translation="MKKFLVLSLVLVFTTITVSAQRGPGDRFRHLRIQQGFRSGEITR
                     LERMHLRKNAVRLNMVQRNARRDGIVTPAERVRIHRLKADPRRDMFRFRHNGRQRVI"
BASE COUNT         2281 a         1569 c         1703 g         2279 t
ORIGIN      
        1 attctctttc tgcctgctca aaaatctttt tctcttttcg tttataccac caatgcggcg
       61 ctgttttttt cattacggta tcgatattac gcataccaac gatgttccaa agccctttta
      121 gtttaccggc taaagaaggc tgcatggccc gcaggctcat ggcaaaacca ctgtcgaggt
      181 attccatatg gtgccaatag cccgagggca tgaaaagtgt atcgccgggt tccaatataa
      241 aatctaaacc ctgcgccagt ttcagtgccg ggaacttttc ataatctact tttccttcct
      301 ggtaatagtt ggaaaaatcg gcgaggctca atacttccca tggtttgcga tagagtttgt
      361 attgttcttt gaaagggaac atcagcactc gttttcttcc gccgaattgt gtatggaaga
      421 tatgcgacat atcaatgtcg aaatgcatat gcgtgatgct gccggcgcca ccggtaaaca
      481 gcatggggta tctttttaca aaaccttttg ccaggtgttc cggccaggta aaatcattga
      541 tcagttgcgg ggcatggtca aaaatattga acaggaaaat ccgccagccg gcagggcctt
      601 tgctgatcat gtcaatgtat tcgccaaagg tcttgtaatc atccgcttta ttgatgggtg
      661 tgtaagcatc actctttaca ttgttgtaaa gcggcacttt tttatcacct accagttgct
      721 tgaagtattc ccagttccac ttggtatagg caggccagtc tttggaaagg tttttgatca
      781 ccaccggtat agctggttca taaaattcct tcttgaacac ttgtggttca atgtgatcgt
      841 aagtggcaac cggccttaac tgcatgtttg taggttgggt ttttacaaag atggggtgaa
      901 aatactaatt gcagggtann nnnnnnnnnn nncatgggcg gaatagcaaa aaccaagtaa
      961 ttttatagat acctgacact atgatactac gcatacatcc gaaagacccg cagccccgcc
     1021 tgatcaagca ggttgttgcc aaattacagg aaggaggcat tattatctat cccactgata
     1081 cagtttacgg tttgggttgt gatattctgc agcagaaagc agtagaaagg atctgccgca
     1141 tcaaaaaggt agagccacgc aaagcacagt tgtcctttgt atgcagtgat ttgtcgcatc
     1201 tgagtgatta cgccaaaccg ctttccaacc cggtgtaccg cttgctgaaa gagcatttgc
     1261 ccggtcccta cacttttata ttaccggcca gtaagatggt gcccaaaata atgcagagca
     1321 aaaaggatac tatcggctta cgaatacccg ataaccggat tgcccaggct attattgaag
     1381 aactgggaag gcccatcctc agcgcatcgt taccgggaga aatggtagaa gattacaccg
     1441 acccggaagt gatgtttgca aattttcaca acgaggtaga ctttgttatt gacggtggta
     1501 taggcggcat gattccttct accgtggtgg actgcactag tgatgaaccg gtggttaccc
     1561 ggatggggct tggggagtgg gaggaaaagt aaaatgaggt tcttcacttt ttatttactt
     1621 ataaaatcca acctcattat tattcctgat tagttctttt actaggaaat caaatatttt
     1681 caccttacag taaccccttt tcataaaatt cagtttaatg gttaatgaaa caggttgtta
     1741 tgggttataa ataataaaat gaaaagcaca atccctcgga tcagtgttca aaatgtccac
     1801 aatagacact cgcattgtat tagctgataa atagtagcca attcctctgc accatgaatt
     1861 agccccccag gcatttacca taattattga agaagaggtt atttgggatg aaactatttc
     1921 ataactcccc aacgaagttt tggaaacagt aaaatttaaa gtacctcctg cctttgttcc
     1981 attttcattt acacggccat agcatacagg taacaggtta tatgctgacc ccgtggctgt
     2041 gcctgttatc ctgccatcat caataacagc acttccgttc acaatcatat taccgttcac
     2101 atgcaatctc tctccgggag atgaagtgcc gatacctgta gtgtttgttg tattattaat
     2161 gaatacttct cctcccaact gtacctgtgc attggcatta acagcagtga gtgaaacatt
     2221 tccggtgctg gtagtttgca atactccggt tgaggtacca ttgaggttca tttgcataaa
     2281 agtgcctccg ctgccggttt tctggaattc aatcatatcc gttccgtcca tacgcagcac
     2341 tacatttcca ttgttattgc cactattggt gccaaaacga aaattatcac cactcaactg
     2401 tacaaatcct ttattatttc caccactttg catttgaaga atagctgaac tgttattaat
     2461 ggtaataccc gtattgctgc caatatttcc ggtgaattgt gcagaggaaa aaccggtaac
     2521 cgctccctgc agtagggagg tttgattcac aaaaagaatt cctgttgaac taataccatt
     2581 gccttctatt acacctgtac ctttaatatc tccatttaca tgcagtttgg aagtaggatt
     2641 agtgacacca atacctacgt tttgagcaaa tggctgaaga atcagcattt tatcatcgga
     2701 ccgtatccat aatgcgtttg aatttgtagg ataaatagat ccggaaaact cattgttttc
     2761 atcaaaaaaa ttaataccat ttccgccaat attcaatgta cgaccatatg aaccgttata
     2821 accaatgccc attttaccgt cattattcat tcttaaaagg gtgtctgcgt tactgtttct
     2881 caacataagt acattacctg atgcactgct ggttgtacct acaccttcca gccgtgcaga
     2941 gggagaatta gtacctattc ccacattttg tgcctgtaaa aaaccgctgt ttaccaggat
     3001 caggaaataa atgataaatt ttttcataac tatatattta taatgaatcc aattcaataa
     3061 gtaactgtaa aagctttcct ttttttaaat catctccgaa ataccgaatg gcctgtggtg
     3121 caatgaattt tcgttcatcg gttgtgatta cggacctgct ggtgtgcagc aggttgggca
     3181 ggtacacaaa ctgcagccat tcggaaaaat catcactgtt atcaatttgc cttttttcaa
     3241 agtcttttac ccattccggc atttcctttt tccaggtgcc ggcttttctc atttcatctt
     3301 ttatctcatc aattttttgg gctatcatgg tggttatcgt catgtcgtac ttgctggtat
     3361 aaaactatca gcagtttttg ttcaggagaa ccgtcaagaa tagaatggcg gtaatcattt
     3421 agtatatggc agcaaacggc cgttcagtta tcaaacggca gattcgttga gccaacggcc
     3481 atacaataga aaaaaggatg tatattttta taccgttaat gccagcatta gctacgatac
     3541 ggattattat ctgccttcta ctgcttcagc aaccagttat tgcacagcag agtggttacc
     3601 ggctgcaaac attgggcacc cgggatggac tattgtcatc caaggtttat gcattaaaac
     3661 aggccagtga ccggcgtttg tggatcggaa ccgaactcgg tgtcagtgtt tatgatggat
     3721 atggctttga caattttcaa tacacaataa caaatgaaac tatcggccgg gtgctttgta
     3781 ttacagaaga tgcacaaaaa ggcatctggg tgggaggtga taagggtttg ttttattctg
     3841 atggtaggaa tatcaggaag gtggaaaagc aaggggccgt acaattagtg gtagaggctt
     3901 tgcataccga cgaaaacgga aatacatgga tcggtgatat cagtgcgtta tataagataa
     3961 caaaagaaca aagcagtgtt gttaaaaata accggggcaa ggctatcaat ctttcacccg
     4021 ttgcttcctt tacacaaaga gtgtttagta tatcctctga ccgccaccaa aatatttaca
     4081 tcggtgctaa taaaggagtt ttcagcataa gacctggtgc tgataaatat gaaactgttt
     4141 gggaaaatcc cgatccggcc cgccctgttc tttcagtggc agctttttcg ccagacagtg
     4201 ttttttggaa ttgtttaaac agtcatccag ccgaaatgat caggggaaaa gtaaagagca
     4261 cattcaccga agattttatt ggcaggaatg tttttaaagt tcatgataaa atttttgcac
     4321 taactacgag tggagtgggg gtatttgcga atggaatagt gaagtcgttg ttttcatttg
     4381 gaaaaattac caataatgcc ctggcagcat tggtggatgc agaaaataat atctgggtgg
     4441 gttcctggga gggtcttcaa aaattcagga aaacaggctt tcgtcaatat tccctgcagc
     4501 acaatgatca gaaagaaact ttttcttttc ttgaaactaa aaaaggggat ctcttattcg
     4561 gcagtaaccg tggactgttg tttcataaaa aagaagaagc gattgtattt aataacggga
     4621 ttgccccgct ttttccgctg gctgaagtaa tgtgtatgta tgaaagtgaa gatgaaagca
     4681 tttgggcggg cagcggttac cagggtataa gccggtttaa aaacggtaag ctttcaaact
     4741 ggaataaaac agggtggctg aaggataata actgtgaggc gctggtaccc acaccggatg
     4801 gaaaactata cgcctgcacc gaaaatggcg tcaccctgat tgatcccgca gctgctgtgc
     4861 caatggttgc ccattaccct tttcaaaaaa agctttcgag ggcgccagaa ctatttggcg
     4921 gttttcatgg tggcaattca gtgtattggt tttacgggag ccgtggattg tattgtctga
     4981 agaatggaca actggtcaat gattctatcc tgggaatgcc ggtaacagat ctttatatca
     5041 ataagatcat tagtgacaaa aaggggaata cctgggtggc tacactggga aaaggattgc
     5101 tctgctgcag gcaggagaat ggccggctgg ttttgaaaaa acaatatgac aaccacaatg
     5161 ggttaccttc caataatgcc ctatcagtac tggctgataa aaatgacaat atctggtgcg
     5221 gggattatat gagcctttct ttgttgatgc atcccggaat gaatgaacat cttttatcgt
     5281 ttactgaaaa agacggtttg ctggagtctt actaccagac attgaaactt gaacagcaaa
     5341 ggaatggtac tatctgggct cttacctcaa tgggtgttgt ttcttttcat cctgatagca
     5401 tcatcctgaa tgataaagca cctgttcttt tgttgaacag aatagtttca gccgatgggc
     5461 aatatgagca tacttcacag catctttctt ttaaaatagg cgctgttcag attcaatata
     5521 ccgctgtttc attaacggat ccttctgcta ccaggtatgc ttaccgggta acagaacagg
     5581 atagtaactg ggtctatacc aataacaggg ttgccgattt tagttttttg cctccgggta
     5641 catatacttt cgaactaaag gccagtaaca ataacaacgt atggacaact gagccgctgc
     5701 ggctcaactt taccattctt ccggcctttt ggcaaacctg gtggttcatg ctggtggtcg
     5761 ttttaattat tgcagccttt gtttatttat ttttcaggca acgaataaga gacataaaaa
     5821 ggaaagcggc aataaaacaa caggtggctg aactggaagc caaagctatc cgggcacaaa
     5881 tgaaccctca ttttattttc aattcactaa atgccataca ggaaagtatt gttacagaaa
     5941 aggtggatgt ggcctatgat tacctttccc gtttttccaa attgctacgg atggtattgg
     6001 ataattcaga aaaaaccatg ataccgctta gcagcgaact ggaaacggtc cgtttgtatt
     6061 tatcgctgga agcaatgcga tttagccagt catttacata cactattgaa gcaggagaga
     6121 atctcgaaaa ggatgatatt ccgtcattat tgcttcagcc ttttgccgaa aatgcgatct
     6181 ggcatggact aattaataaa gagggagaaa agaaactgct tattcggttt gaagaaaaga
     6241 atgatgtgct ggaatgtgta attgaagata atggagttgg aaggacaaaa gcggcagcaa
     6301 tcaaacagca gaagcttgga tctgcaaggt ttgcctcgaa aggcacaaga cttgcgatgc
     6361 aaagaataga gatattgaac agggagcggc cgggttctgc cagtattgaa acaattgacc
     6421 ttcttgatga agcaggaaac gctgcaggaa caaaagttgt tattagatta gcaactgaac
     6481 agctgcataa aaatgaatgg cgaccatgat taagatattg atcattgatg atgagcaatc
     6541 tgcaggacgc atcctaaaag tgctgatcga aaagcatatc ccgggagaaa agcagattga
     6601 ggtatcgaat gatcctgctg aggcattgca aattctgaaa gaataccaac caaccctggt
     6661 aatgctggat attgaaatgc cgggtatgaa cggctttgat tttttaaaca gggccagcga
     6721 atgggatttt gatgtcattt ttacaactgc ctatgacaaa tatgccatca aggctatccg
     6781 cttcagtgct ttggattacc tgttgaaacc ggttgatatt gttgagcttc aaaatgcagt
     6841 caacaggcat attgtcaagc aacaggcacc gctcaaagaa aacaggcaac tggtcaataa
     6901 tttaatatat aacctgcagc aaaaagatca gcagcagttc aaacttgcct tatcaaccag
     6961 tgaaggcata ttcttatatg atcctaaaga catcattgca ctcgaaggaa gcagtaatta
     7021 cacccggttc ttttttgcag agcataagcc gcttattgta gccaaaacac ttaaggagta
     7081 tgaggatata ttgacggaac accagttttt gagagtacac aaatcttacc tggtcaataa
     7141 actacatgtg ctgaaagtag acagggatgg cattctggaa atgagtaacc agcagcatat
     7201 acccatatcc cgccgtcgta aagcagaaat attgcaatgg tttggaaaaa aataagcacc
     7261 attaaaaatg gtgctttcct gcctcacgat tcttttgata tatggtggtt tactattttc
     7321 aaatcacccg ttgacggcca ttgtgccgga aacggaacat gtcgcggcgg ggatctgctt
     7381 tcaggcggtg aattcttact ctttctgccg gtgtcactat tccatctctg cgggcattgc
     7441 gttgtaccat attgaggcga acagcatttt ttctcaggtg catacgttct aatcttgtta
     7501 tttcgccgct acggaaaccc tgttgtattc tcaggtgacg gaaacgatcg ccgggacccc
     7561 gctgtgctga aacggtaata gttgtaaaaa ctaaaaccaa gctaaggact aaaaactttt
     7621 tcattttttt cttatttgtc tatatgacaa aaaaggagct tccaggttta atagaaaaaa
     7681 atgtatcaga ataaatcagc ctggatactt tttaccagga atgttttccg gtggtatttg
     7741 cactggccat actgttccat ggctttaata tgattggctg tgccataccc tttattattc
     7801 aaccagtcgt aatgcggaaa ttcttcatgc agctgcagca tgtatt
//
LOCUS       DAORLS010000138         7795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5062_length_7795_cov_2.042248, whole genome shotgun sequence.
ACCESSION   DAORLS010000138 DAORLS010000000
VERSION     DAORLS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_7795_cov_2.042248"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>229)
                     /locus_tag="PK977_11090"
     CDS             complement(<1..>229)
                     /locus_tag="PK977_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18710.1"
                     /translation="VKSAVWDINTQKLSLIYNTAVITLEKIQCRIADRGHDNDFELAK
                     DAVYNALPACCRYRELNASPANSETGESKEGE"
     assembly_gap    230..253
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(422..1252)
                     /locus_tag="PK977_11095"
     CDS             complement(422..1252)
                     /locus_tag="PK977_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18711.1"
                     /translation="MKKLFILLLLFTAFRMSGQELYVFSEPASNVPAHSLSVKLTNHF
                     VTSDKIYGRFSNRLMPQVYLGVSKKFMVAAGGTFANMHTPNFRYESVNLYAKYRFLSK
                     DELHKHFRMAVFAEASSTRAPFHYDEITLMGDKSGVELGVIATQLWHKLALSGTVAHT
                     QVLHESRTNKVLYIPARNYQSMNYAISAGYLVLPKEYKDYRQTNLNLYAELLSQQTLD
                     RSTYFVDLATAMQLIFNSNAKLNLGYRVQLGGNMQRMTSNSWLISFERTFLGALKKKK
                     "
     gene            complement(1265..1756)
                     /locus_tag="PK977_11100"
     CDS             complement(1265..1756)
                     /locus_tag="PK977_11100"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF18712.1"
                     /translation="MTGLQKQWFVLLIALCPLLAKAQIRYGDDAQEIALPAVNGDTLK
                     LSSLKGKVVLLDFWASWCGPCRVANKGLVKLYGKYKSKGFEIYGVSFDKDKSDWKKAI
                     AKDKITWLQVNDDGGWESPTAISWGIDAIPTSYLIDQNGKLVAMDLTGKELEKKLKEI
                     LED"
     gene            complement(1813..2358)
                     /locus_tag="PK977_11105"
     CDS             complement(1813..2358)
                     /locus_tag="PK977_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRF18713.1"
                     /translation="MKKLFIIGIFTLFIQRADAQFSKADLQATGLTCAMCSNAINKAL
                     QKVSFVESVESDIKNSSFAIVFKKDAVVNIDALKEAVEDAGFSVGGLRLTGLFNEVKI
                     ASDQHIKIGNSHFHFISVKDQVLNGEKTVTVVDKNFISEKQFKKYSSATKMDCIRTGK
                     AGTCCRNEGLAEGERVFHVTI"
     gene            complement(2379..>2847)
                     /locus_tag="PK977_11110"
     CDS             complement(2379..>2847)
                     /locus_tag="PK977_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRF18714.1"
                     /translation="IYGNCEMCKTTIEKAGNVKKVASVDWNKDTKELTVKYSSSSTNA
                     AKIQQKIAEVGYDNVGFKATDEAYNKLHGCCKYDRGAATEEVKECCKGGKCDKEGHEG
                     KDCCKKDGEKMSCDKEGHSGKDCCKKDDKKMDCCKDGKCSKEGHDGKDCCKKS"
     assembly_gap    2848..2954
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3053..3445)
                     /locus_tag="PK977_11115"
     CDS             complement(3053..3445)
                     /locus_tag="PK977_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18715.1"
                     /translation="MKKGLAAISLLLYFAVTSGIVINSHYCMKRLVSIHLFETKAKVC
                     GLCGMDIHDSEGCCRDEVKVIKLVQDQVKIPVTLYEIPSVEAVVPVMTDYLLASLRKI
                     DGQRHFNNHSPPILSAQDTYLQNNVFRI"
     gene            complement(3517..>3955)
                     /locus_tag="PK977_11120"
     CDS             complement(3517..>3955)
                     /locus_tag="PK977_11120"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18716.1"
                     /translation="IRIGKRWNWHADIYFQQKIGDAPVNLPVIYTRNRIAYEGNLGFK
                     NLDFAGGIEVRYRATYKADGYSPVLGKFFYQDSIRIRNELPDISAYIHFRIRPFKAFI
                     RFENLNTARNLDGFSFTRNNLVAPGYALPGLQIRTGVYWSFVN"
     assembly_gap    3956..3971
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3972..>4661)
                     /locus_tag="PK977_11125"
     CDS             complement(<3972..>4661)
                     /locus_tag="PK977_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18717.1"
                     /translation="PRLRFEHTFRLDQNKYIFQDYLADSVYYKTYYDTALRRPVDTFL
                     VREVWKVITNDFSIYQFPDIKNLKQFIKLGLTVQNFTGEFASGKNTFYNLSGHAEYRN
                     KSKNQQWDIEANGRLYFTGFNAGDYEAHISLQRFAGKKLGYIQLGFENVNRTPSFFFD
                     SRSSFYLMKTVQDFKKENTTHLFASYYLPSFKFRLTGHYYLLTNYSYLEDFYLLKQES
                     TLFNILQVTLQ"
     assembly_gap    4662..4680
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4681..>5124)
                     /locus_tag="PK977_11130"
     CDS             complement(<4681..>5124)
                     /locus_tag="PK977_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18718.1"
                     /translation="YQLGSRVEQVIDLVHTQNLKPNWNAAFRYRLINSPGIFKNQKTN
                     HNNYLLNSFFQSNNKRYSNYFVLLGNKMQSAENGGIQDTADFLNDPIFKDRFNIYTKI
                     GGDDPFSSNFFTTKINTGNKYNEFSVLLRQQYDLGKKDSIVTDSTV"
     assembly_gap    5125..5139
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5140..>5462)
                     /locus_tag="PK977_11135"
     CDS             complement(<5140..>5462)
                     /locus_tag="PK977_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18719.1"
                     /translation="PPDWRRWQNWWWYDSLKQRNKNEDSITIYYRYLDSAGTYKLDSS
                     IGDFTRRFPLPATHIILGNLGNASRSLLFSPVMSSGFNPGFTAFDIYQFKMENVRFYN
                     TTRPYS"
     assembly_gap    5463..5501
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5635..6459)
                     /locus_tag="PK977_11140"
     CDS             complement(5635..6459)
                     /locus_tag="PK977_11140"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRF18720.1"
                     /translation="MGKKAMKQLNEAAKFLKKQYAGVPTVGIVLGSGLGNFTEEIEVE
                     KEVAYEDIPHFPVSTVSGHKSKLVFGKLSGKTVVAMAGRFHFYEGYKAHDIVFPIRVM
                     KLLGVETLLLSNAAGSVNPDYKVGDLMIIKDHISFFTPNPLIGKNMEELGPRFPDMSE
                     PYKKSLIDKAKAISVAHNYNVKEGVYVAVTGPTFETRAEYVFIKVAGGDVVGMSTVQE
                     CIAANHMGMQVFAMSVVTDVGLQEVHAVISHEEVLEAAKRAEPKLATIFKELVAAI"
     gene            complement(6521..>6784)
                     /locus_tag="PK977_11145"
     CDS             complement(6521..>6784)
                     /locus_tag="PK977_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18721.1"
                     /translation="ISGKINRDGRPNRVAIYGWHKTDGKPIQPLYTGHINWWVDYSQG
                     IRLVWRKIKVGNKWMDYTAVLKDPVLQKLLCDETFCDFYRYDY"
     assembly_gap    6785..6845
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6846..7276)
                     /locus_tag="PK977_11150"
     CDS             complement(<6846..7276)
                     /locus_tag="PK977_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18722.1"
                     /translation="MKKIQWPERSSSLGGTAFYQQAAAMKWAARDSCVLKEILAGNAP
                     SFLKAFVPVRVAVLDSATGKTIHATFYVAPDYLAVGTNDDWARVNITPMAAQQIADKL
                     DCFLPTRKMVDDIYKAAKLKLEPVPMYAFRDSTPTMWHHHL"
     gene            7406..>7795
                     /gene="sucC"
                     /locus_tag="PK977_11155"
     CDS             7406..>7795
                     /gene="sucC"
                     /locus_tag="PK977_11155"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HRF18723.1"
                     /translation="MNLHEYQAKELLKKYNVPVQEGFACSTVHEAEEAYRQIKTQTGS
                     KFAVIKAQIHAGGRGKGTIKENGINGVKVAKSLEDVQDFASKILGGTLVTLQTGPAGK
                     VVNKIFVAADMYYDGPSERKEFYLSILL"
BASE COUNT         2116 a         1757 c         1458 g         2183 t
ORIGIN      
        1 ttcaccttcc ttgctttctc ctgtttcact atttgccgga gaagcattta gttcacgata
       61 acgacagcag gcaggcaatg cattatagac tgcatctttt gccagttcaa aatcattgtc
      121 atggcccctg tccgcaatcc tgcattgtat cttttccagt gtaatgaccg cagtattata
      181 aatgagagaa agcttttgtg tattgatatc ccagacagca gacttcaccn nnnnnnnnnn
      241 nnnnnnnnnn nnntttctca attctttcct tgcacatttc gcaggcgccg gccacaaaaa
      301 tcactgccgt ggaatctttt gatgactgtg ccatcacttt cccaaaagaa agcatactga
      361 tagccacaag ggctacactt atatatttaa ttgacatttt ttttgtttta aatgaactac
      421 atcatttctt cttcttcaat gcacccagga atgttcgttc aaaactgatc aaccagctat
      481 tggaagtcat acgttgcata ttaccgccca attgtacacg gtaacccaaa ttcaatttgg
      541 cattgctgtt aaagatcagc tgcatggcag tggcgagatc gacaaaataa gtactcctgt
      601 ccagtgtttg ctgtgacagc agttcggcat acaaattcag gttggtctga cgataatctt
      661 tatactcttt cggcaaaact aaatagcctg cagatatagc gtaattcatc gattgataat
      721 tacgggcagg tatatatagt actttgtttg tcctcgattc gtgaagcacc tgtgtatgtg
      781 ctactgtacc ggaaagagct aatttatgcc atagctgagt agcaataaca cccagttcaa
      841 caccactctt atctcccatc agggttattt catcataatg aaaaggagcc cttgtggagg
      901 aagcctctgc aaacaccgcc atccggaaat gtttatgcaa ctcatctttg gaaagaaagc
      961 gatatttcgc ataaagattc actgattcat accggaaatt aggagtatgc atattcgcaa
     1021 acgtaccacc ggctgccacc atgaatttct tgcttactcc taagtaaacc tgtggcataa
     1081 gcctgttaga aaaccgcccg tatatcttat cactggttac aaagtgattg gtgagcttta
     1141 cactcagtga atgggccggc acattactcg ccggttcaga aaatacatac agttcctgtc
     1201 cgctcattcg aaaagcagtg aacaataata gcagtatgaa cagttttttc atgcttacta
     1261 tctgttagtc ttccaaaatt tctttaagct ttttttccag ctctttcccg gtcagatcca
     1321 tcgctaccag ttttccattt tgatctatga ggtaagaagt aggaattgca tcaatacccc
     1381 aactgatggc tgttggcgat tcccatcctc catcatcgtt cacctgcaac caggtgatct
     1441 tatctttagc tattgccttt ttccagtctg atttgtcttt atcgaacgac acgccgtata
     1501 tctcgaaacc tttggattta tacttgccat agagcttaac aagtccttta ttggccaccc
     1561 ggcatggccc gcaccaggat gcccagaaat ccaacagcac cacttttccc ttcagtgaag
     1621 acaatttcaa tgtatcgccg ttcacggcag gtaaagcaat ctcctgcgca tcatcgccat
     1681 acctgatttg tgcttttgcc agtaaagggc aaagagctat cagcaaaaca aaccattgtt
     1741 tttgtaagcc tgtcatagcc ttcagttgaa aaaagtttct ccctcctctt tgaagaggga
     1801 gaaacaaaaa gatcagatgg ttacatgaaa tactctttcg ccttcagcaa ggccctcatt
     1861 cctgcagcaa gttcctgcct tacctgtacg aatacaatcc atcttagtgg ctgaactgta
     1921 ttttttgaac tgtttttctg aaataaagtt cttatcaact acggtaacgg tcttttctcc
     1981 atttaatacc tggtctttca ccgagataaa atgaaaatga ctattgccga tcttaatatg
     2041 ctgatcagat gcaatcttta cttcattaaa caacccggta agccggaggc cgcccacaga
     2101 aaagcctgca tcttctactg cctctttcaa tgcgtcgata ttaaccaccg catctttttt
     2161 gaaaacaata gcaaaagagg aatttttgat atctgattca acagactcaa caaaacttac
     2221 tttttgcagg gctttattaa tagcattact gcacatggca caggtaagtc ctgtggcctg
     2281 caggtcagct ttgctgaact gagcatcggc ccgctgaata aaaagagtga atattccaat
     2341 gatgaataac ttcttcattc ctgttgctta tagtttgctt atgacttctt gcagcaatct
     2401 ttaccatcat ggccttcttt actgcacttg ccatctttac agcaatccat tttcttatca
     2461 tcttttttgc aacaatcttt accgctgtgg ccttctttat cgcagctcat tttctcacca
     2521 tcttttttgc agcaatcttt accttcatgg ccttctttgt cacatttacc gcccttgcag
     2581 cattctttca cttcttcagt agcagctccc ctgtcgtact tgcagcagcc atgtaattta
     2641 ttgtaagctt catctgttgc tttaaaccca acattatcgt aacctacttc agctatcttt
     2701 tgctgtattt tggcagcatt ggtagaactg ctactgtatt taacggtcaa ttctttggtg
     2761 tctttattcc agtccacgct ggctaccttc tttacattac ctgctttttc gatggtggtt
     2821 ttacacattt cacaattacc gtagatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnntttgag ttgtaataaa tatttgaatg aataagatgg taacgaccat
     3001 tatagtatag tcactcatcc agtaggatag aatggaaaat tattaccggt tctcaaatcc
     3061 ggaaaacgtt attctgcaag taggtatcct gtgcagataa tataggagga gaatgattat
     3121 taaaatgacg ctggccgtca atttttctga gagatgcaag caaatagtct gtcatgacag
     3181 gtacaactgc ttctacagat gggatctcat aaagcgtaac aggtatcttc acctggtctt
     3241 gcaccagttt gatcactttt acttcatccc ggcagcagcc ttcagaatca tgaatatcca
     3301 ttccgcagag accacaaact tttgccttcg tctcaaacag gtgaatggaa accagccgct
     3361 tcatgcagta atgcgaattg atcacaatac cgctggtaac agcaaagtac aaaagtaatg
     3421 atatagcggc gaggcctttt ttcatgaaca gtacaaaagt aatattaatc ccggatttga
     3481 aaacaaaccg ggctaatgat ctttatcatt tgataatcaa ttaacaaaac tccagtaaac
     3541 tcccgttctg atctgtagcc cgggcagggc ataccccggg gccaccaggt tattacgggt
     3601 aaaactgaac ccatcaaggt tgcgggccgt attcaggttt tcaaaccgga taaacgcttt
     3661 gaacggcctg atgcgaaagt gaatgtaggc tgatatatct ggcagctcat tacggattct
     3721 aatgctgtct tggtaaaaga atttacccag caccggcgaa tacccgtctg ccttataagt
     3781 tgcccggtaa cgaacctcga taccaccggc aaaatcaaga tttttaaatc caaggtttcc
     3841 ttcgtaagct atccggttgc gggtataaat gaccggcagg ttcaccgggg catcgccgat
     3901 cttttgctga aaataaatat ctgcatgcca gttccagcgt ttgccgatcc tgatcnnnnn
     3961 nnnnnnnnnn ntctgtaatg ttacctgcag gatgttaaac aaagtgcttt cctgcttcag
     4021 taaataaaaa tcttcgagat agctgtaatt ggtgagcagg taataatgac cggtaagcct
     4081 gaacttaaac gacggtaaat aatatgaagc aaataaatgt gtagtgtttt ccttcttgaa
     4141 atcctgaact gtcttcatta aataaaaact gctgcggctg tcaaaaaaga aagagggtgt
     4201 acggtttaca ttttcaaaac ctaattgtat ataacctaat ttcttaccgg caaatctttg
     4261 taaactaata tgcgcctcgt agtccccggc attgaaaccg gtgaaataga gccggccatt
     4321 ggcttcaata tcccattgct ggtttttact tttattccgg tattcagcat gaccggacaa
     4381 gttatagaaa gtgtttttac cactggcaaa ctccccggta aagttttgaa cggtgaggcc
     4441 cagtttaata aactgtttca ggttcttgat atccggaaac tggtaaattg aaaaatcatt
     4501 ggtgatcact ttccacactt cccttaccag aaaagtatca acaggccggc gcaatgccgt
     4561 atcgtaatac gttttataat acacagagtc cgccaggtaa tcctggaaaa tatatttgtt
     4621 ctgatcgaga cggaaagtat gttcaaaacg aagacgggga tnnnnnnnnn nnnnnnnnnn
     4681 taccgttgag tccgtaacga ttgaatcttt ttttcccagg tcatattgct gccggagcag
     4741 tacactgaac tcattatact tgtttcccgt attgatcttt gtggtaaaaa agttagaaga
     4801 gaaaggatca tcaccaccga tcttagtata aatattaaaa cggtctttaa aaatagggtc
     4861 attcaaaaag tcagctgtat cctgtatgcc gccgttttcg gccgactgca tcttatttcc
     4921 caacaaaaca aaataattac tgtatcgttt gttgtttgac tggaagaaag aattgagcag
     4981 gtaattatta tgattggtct tctggttttt aaaaataccc ggtgagttga tcagccggta
     5041 acggaaagct gcattccagt tcggcttcag gttctgcgta tgcaccaggt caataacctg
     5101 ctctaccctg ctacccagct ggtannnnnn nnnnnnnnng aatacggcct tgtggtatta
     5161 taaaaccgga cattctccat tttaaactgg taaatatcaa aggccgtaaa gcctggatta
     5221 aatcccgaag acatgaccgg tgaaaacaac aacgaacggg atgcattgcc cagattacct
     5281 aaaattatat gcgtggccgg taaagggaat ctccgggtaa aatcgccaat agatgaatcc
     5341 agtttgtaag tacctgctga atcaagatac cggtaatata tagttatgga atcttcattc
     5401 ttgtttcgct gcttcaggct gtcgtaccac caccagttct gccagcgccg ccaatctggc
     5461 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgacaaca gcaagatggc
     5521 aactatgaat aaatatgtaa acttttttac cagatagtga ttttgaacgc agtgaataaa
     5581 aaaacctttg caagataaac gctgcaaagg tataaaccgg cagtaaactg ttaattaaat
     5641 agctgccacc agttctttaa atatcgttgc cagtttaggt tctgcccgtt tagctgcttc
     5701 cagcacttct tcatgcgaaa taacggcatg tacttcctgc aagcctacat cggtaaccac
     5761 actcatagca aacacctgca tgcccatatg atttgcggcg atacattcct gcacggtact
     5821 catccccacc acatctcctc ctgctacttt gatgaacaca tattctgctc ttgtttcaaa
     5881 cgtaggaccg gtcaccgcta catacactcc ttcttttaca ttataattat gtgcaacgga
     5941 aatagctttt gctttatcaa tcaaactttt tttataaggt tcactcatat ccgggaaccg
     6001 gggtcccagc tcttccatat tctttccgat cagcgggttg ggggtgaaga aactgatatg
     6061 atctttgatg atcatcaggt cgcctacttt gtaatccggg ttgactgacc ccgctgcatt
     6121 ggacaataac aaggtctcca cacccagcag tttcatcacc cgtatgggaa acacgatatc
     6181 atgggcctta tatccttcgt aaaaatgaaa gcggcctgcc atagccacca ccgttttgcc
     6241 gctgagtttt ccaaaaacca gtttgctctt atgaccagaa acagttgata ccgggaaatg
     6301 agggatatct tcataggcca cttccttttc cacctctatc tcttctgtaa aattgcccag
     6361 cccgctgccg agaacgattc ccacagtggg aacacctgca tattgttttt tcagaaactt
     6421 agccgcttcg ttcagttgtt tcattgcttt ttttcccata gccattcact cccgattttg
     6481 aaaaaaggga agcagcaaac gtaagaaaaa aatccggggg ctaataatca taccggtaga
     6541 aatcacaaaa ggtttcatcg cataacaact tttgcaatac cgggtctttc agcacggctg
     6601 tataatccat ccatttattt ccaactttta tttttcgcca gaccaatcgt attccctggc
     6661 tgtaatctac ccaccagttg atgtgaccgg tatacaaagg ctgaataggc ttaccatccg
     6721 ttttatgcca gccataaata gctacacggt tgggccggcc atcccggttt atctttccgc
     6781 tgatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnatcag gtgatggtgc cacatggtgg gtgtactgtc cctgaaagca tacattggca
     6901 ccggctctaa cttcagtttg gccgctttgt aaatatcatc caccattttc cgggtaggca
     6961 gaaaacaatc cagtttgtct gctatctgtt gtgcggccat cggggtgatg ttgactcttg
     7021 cccaatcgtc attcgttccc accgccaggt aatcaggcgc aacataaaaa gtggcatgga
     7081 tcgttttgcc ggtagcagaa tccagaacag ctacccgtac cggaacaaac gcttttaaaa
     7141 aagaaggagc attgcctgcc agtatttctt tcagtacaca agaatccctt gctgcccatt
     7201 tcatcgccgc agcctgctgg taaaaggccg ttccgccaag ggatgaactt ctttccggcc
     7261 actgtatttt cttcaccggc tggcaggaga taataaggac aaatagcaga ggcaacagac
     7321 gaatcattcc acaaggtaaa aaaaccataa accagaaacc agaaactagc aacttagatt
     7381 tatcttcgcc gaaatttttc cagatatgaa cctccacgaa taccaggcca aggaattatt
     7441 aaagaaatac aatgttcccg tgcaggaagg atttgcctgc agcaccgtac atgaagccga
     7501 agaagcttat cgccagatca aaacgcaaac cggcagcaag tttgccgtta tcaaagctca
     7561 gatccatgcc ggcggccgcg gaaaaggcac catcaaagaa aatggcatca atggcgtaaa
     7621 agtggccaag tcgctggaag atgttcagga ttttgccagc aagatattag gcggcacact
     7681 ggttacgctg caaaccggcc ctgccggcaa agtggtcaac aagatattcg tggcggcgga
     7741 tatgtattat gatggcccca gcgaacgcaa agaattttat ctctccatat tgctc
//
LOCUS       DAORLS010000139         7742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5110_length_7742_cov_1.834526, whole genome shotgun sequence.
ACCESSION   DAORLS010000139 DAORLS010000000
VERSION     DAORLS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5110_length_7742_cov_1.834526"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1269)
                     /locus_tag="PK977_11160"
                     /pseudo
     CDS             complement(1..1269)
                     /locus_tag="PK977_11160"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
     gene            complement(1450..>1902)
                     /locus_tag="PK977_11165"
     CDS             complement(1450..>1902)
                     /locus_tag="PK977_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRF18724.1"
                     /translation="NTTYLTRVMGEAVNKLINGESIKSIGVADVKYVEKKALKPMLDN
                     NLIEGQIIFIDSFENVIVNITHEQFEEQRKGRGFRIVFKRDEVIDRISDTYADVPEGE
                     KLALFNSAGYLEIAINKGNAAGLFGLKGYNDKAMQSQLSYQTVKVFFE"
     assembly_gap    1903..1912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1913..2262)
                     /locus_tag="PK977_11170"
     CDS             complement(<1913..2262)
                     /locus_tag="PK977_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRF18725.1"
                     /translation="MPLITLTSDIGSPDYLVGAIKAQLLHLNPEFRIVDITHNVPPFN
                     YSQAAYVCRASLRNFPDFSYHLILVNLFEKKPEQLLLAFHNNQYILCADNGLLTMMLE
                     ETPEIVIGLPLDDTA"
     gene            complement(2284..>2968)
                     /locus_tag="PK977_11175"
     CDS             complement(2284..>2968)
                     /locus_tag="PK977_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF18726.1"
                     /translation="KLYSLQTEYPSSPLHTMVADVSSENDCRRFIEVTIKVFGGIDVL
                     INNAGVSMRALIKEAEVDVIRKVMDINFYGTVYCTKYALQSIIERKGVIVGVSSIAGY
                     RGLPGRSGYSASKFAVQGWLEAIKTELVADGVHVMWVCPGFTTSNIRNAALNKEGSSH
                     GETPMDESKMMTAEECAVHILKAVRKRKRTLVLTFTGKRTVFMQKFFPKLADNLVRKF
                     FFKDGNLVK"
     assembly_gap    2969..2997
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3184..3256)
                     /locus_tag="PK977_11180"
     tRNA            complement(3184..3256)
                     /locus_tag="PK977_11180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3221..3223),aa:Lys,seq:ttt)
     gene            complement(3348..4181)
                     /locus_tag="PK977_11185"
     CDS             complement(3348..4181)
                     /locus_tag="PK977_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18727.1"
                     /translation="MRLLLSIYTLLLIGRLQAQPVNEVNLASNAFYIPAEKTILYKLG
                     DKTIPLLISQYGNSSEIVCINLHDNEFTSVQAARSVLEQKGGLLIKLDNSRQRVIRFR
                     LRGRNYAFDPNRIFSRVGIEQTLRENSTADPYAIEEIEKFATRILNLIPEETICVVAL
                     HNNTENAYSIKSYLPGNEREKDAREVYANENQDIDDIVLTTDSMLYKKMADCGYNSVW
                     QHNERAKKDGSLSVFYGEKNKRYINIETQHGRLSQYIEMFGKLMDILNDETKGGQFSP
                     D"
     gene            complement(4253..5191)
                     /gene="plsX"
                     /locus_tag="PK977_11190"
     CDS             complement(4253..5191)
                     /gene="plsX"
                     /locus_tag="PK977_11190"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRF18728.1"
                     /translation="MTIGLDMMGGDYAPMEAVKGVKYWLSGNDKPVNLLLIGDEPAIQ
                     KLLTEHDVPSDHITIVHAEEIIDMHEHPTKALREKQNSSIAIGFHLLASGKMDAFISA
                     GNTGAMLVGTLFSIKALEGVIRPTISTIIPKENGGTGLLLDVGLNADCKPEQLNQFAI
                     MGSVYAQLVLGIEKPKVGLINVGEEEGKGNLLAQATYPLLKENKHIHFIGNIEGRDVL
                     MDKADVMVCEGFTGNIILKLAESLYEIGQRKKIDDEYFDRFNFEIYGGTPVLGVAKPV
                     IIGHGISGAKAFMNMIKLSQLMLEKDVMQKMKEELA"
     gene            complement(5274..>5466)
                     /gene="rpmF"
                     /locus_tag="PK977_11195"
     CDS             complement(5274..>5466)
                     /gene="rpmF"
                     /locus_tag="PK977_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRF18729.1"
                     /translation="PNPKRRHSQQRSAKRRTHYKATSATLSTDSTTGETHLRHRAHVS
                     EGKLYYKGKVVAEKSPVNK"
     assembly_gap    5467..5498
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5506..6051)
                     /locus_tag="PK977_11200"
     CDS             complement(5506..6051)
                     /locus_tag="PK977_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRF18730.1"
                     /translation="MSRRREFEIAFVGLKPGVHEYNYSIDDKFFEPFQQQDFHNCKAN
                     IRLLLDKKSSFMMLKFEIGGTLEVTCDRCNNNLPLELWDEFNITVKMVEEPELMNDQE
                     DDPDVYYISRGESHLDVANWIYEFINLTIPMQKVCEFEKMDGPHCNPAALDVLKKMEP
                     NEKESKENPIWKGLEKFKDLD"
     gene            6190..>6546
                     /locus_tag="PK977_11205"
     CDS             6190..>6546
                     /locus_tag="PK977_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HRF18731.1"
                     /translation="MQQFLTRLFARLMKSWSWVVLIIFTILIKWASWYPGWVERNYSY
                     GIYPLISKIQRILFGWIPFSVGDIFYGFLVLVIIFRTFKFFKYLFQRRLTRQYFVIAM
                     QQFIFFVLFVYVFFNLL"
     assembly_gap    6547..6662
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6663..7303
                     /locus_tag="PK977_11210"
     CDS             <6663..7303
                     /locus_tag="PK977_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HRF18732.1"
                     /translation="YAAFVTPAQRDSFNRKRDLFASAHKAYESAASNHKFLQYSSRSV
                     KPSLFSYLGNYLGFQGYYNPFSGEGQVNTTIPRFMEPFVTSHEIAHQLGYAKENEANF
                     AAYLACRSYENNAFRYSMYYDMYNYAIVEVFRRDTTLGKLIQELAHPQVKKDQKEFRD
                     FYKKYNNPIEDLVMWGYGHFLKANNQPAGKRSYNEVVAWLIAYYKKYGIEAL"
     gene            7334..>7742
                     /locus_tag="PK977_11215"
     CDS             7334..>7742
                     /locus_tag="PK977_11215"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRF18733.1"
                     /translation="MSNIVKGIVAIAIPLLVGGASGFFTITGVDSWYQTINKPSWNPP
                     NWVFGPVWTTLYIMMGIALFLVWKEDTSEELKKIAIALFSVQLILNFFWSFIFFNLQQ
                     PGWALVEIIAMWFFILLTIFAFVQVNKTAAWLLV"
BASE COUNT         2089 a         1640 c         1514 g         2312 t
ORIGIN      
        1 tttcattttc acatagccgt tatcatcata gtcgtaatcg gcagaaaaat gcggcccgta
       61 aaccaatgga gcttcaccat attgttcacg gctcagatag taaacaaggt tgattgggtt
      121 gtcaacatta ttcatatcta ctgccgggtt agcattggag cgtatcatgg tggtgaggta
      181 catcatgtaa cccagcatca taaagagata acaccagaga gacagtttta caatttttat
      241 tgcactggct ttaataaagt atcctgctgc tgctgccacg attaatagca ataaaagttt
      301 tcctatgctg agacctgcac ctggtttaat gacaaacgga agagcagaca agaaaaggaa
      361 taaagcaaac caaatgatca ttacagtagg cgaaaccttt gtttctttta aacgcaatgc
      421 ccaaataata accgccgcca atgccaggaa gtaaatggca aacccggcaa agaaggggag
      481 gccgaaagag ttaacaaaga atacatcaaa ctgtcctgct gctttcatgg agtattgaat
      541 gacacctacc tgaaccaggc ctgtaatcaa gcagccaata ataaatgcgg tgatagcccc
      601 tttagtagta ggctgataac gacggtaata ataaatcatt acaattgccg gtataaccag
      661 caagttcaac aagtgtaccc cgatagataa acccatcatg aagaaaagga aaacaatcca
      721 ccggtcgctc cttgcccttg catgaggatc gttaccggca tgttcatctg catgttccca
      781 tttcagcata gcctagaaca ctaatgcggt aaagaaagat gataatgcat atacttcacc
      841 ttctaccgca ctaaaccaga acgaatcact gaaggtgtag gcaagtccgc caactacacc
      901 ggcggccatg gttgtaaagg tttgctgtga tgtcaattct tcaccaacac tgacaaacat
      961 tttacgggca aaatgagtga tactccagaa aaggaaaagt atagtggcag cacttgccaa
     1021 agcactcatg aagtttacag cattggcagc tgtttgtccg ttatctccaa acaggatgat
     1081 aaagaaacgt cccaataaaa caaacaatgg agcacccggc gggtgtggca attgaacttt
     1141 gtctgcactg gcaacgaatt caccgcaatc ccagaggctg cctcttgctt cacgggtcaa
     1201 aaagtaagtg gcaaaggcaa caaagaaaac tgcccagcct gtaaggttat tgacttttct
     1261 gaaattcata aataaacaat tggttttaac tcctttgcaa actgtggttg gcccacagac
     1321 cgaaggaaag caaaaatagg gtttcagtgg tagatttcag ttgcgtcggg gtagttttag
     1381 gaatttaggt taccaccggt tatggttgga gaaaaaagga ttttattagt gctggaaagc
     1441 agtgaaattc tattcaaaaa acaccttcac tgtttgataa gatagctggc tttgcatggc
     1501 tttatcgtta taacctttaa gcccaaaaag tccggcagca ttgcctttat tgattgcaat
     1561 ttccaggtac cctgcactat tgaacagggc cagcttttca ccttccggta catctgcata
     1621 ggtatcactg atacggtcaa tcacttcatc tcttttgaat acaatcctga atcctcttcc
     1681 tttgcgctgt tcttcaaatt gctcatgggt gatattgact attacatttt caaagctgtc
     1741 aatgaaaatg atctggccct caatcaggtt attatccagc attggtttaa gggctttttt
     1801 ctccacatat tttacatcag caacgccaat actttttatt gactctccat ttatcagttt
     1861 atttacagct tctcccataa ccctggtgag gtaggtggta ttnnnnnnnn nngcagtatc
     1921 atccagcggc aggcctatta ctatttccgg tgtttcttcc agcatcattg taagcaagcc
     1981 attgtcggcg cagagtatat attggttgtt atgaaaagcc agcagcagtt gctccggttt
     2041 tttctcaaaa agattaacca gaatcaggtg atagctaaaa tcaggaaaat tgcggaggct
     2101 ggcacgacaa acataagccg cctgtgaata gttaaatggc ggtacattat gtgtaatgtc
     2161 tacgatcctg aattccgggt taaggtgaag caattgtgcc tttatagcgc ctaccaggta
     2221 gtcggggctt ccaatatctg atgtcagggt tattaaaggc aagggttaat gatttatata
     2281 ctcctattta acaaggttcc catctttaaa gaagaacttt ctgaccaaat tgtctgctaa
     2341 ttttggaaag aatttttgca tgaataccgt tcttttccct gtaaaagtga gtacaagagt
     2401 tctttttctt tttctgacag cctttaaaat atgcactgca cattcctctg ctgtcatcat
     2461 tttactttca tccataggtg tttctccgtg agaactccct tctttgttca gggctgcatt
     2521 ccggatatta gaagtggtga aaccaggaca cacccacatg acatgcaccc catcggctac
     2581 caactctgtt ttaatggctt ccagccatcc ttgtacagca aacttgctgg ctgagtatcc
     2641 gctcctgccc ggaaggcccc gatagcctgc gatggaagaa acccccacaa tgactccttt
     2701 tctttcgatg atagactgca aagcatactt ggtgcaatag acagttccat aaaagttaat
     2761 gtccattact ttccggatca catcaacttc tgcttcttta ataagggctc gcatagaaac
     2821 accggcatta ttgatcagta catcaatacc accgaaaacc ttaatcgtca cttcaatgaa
     2881 tcggcggcag tcattttcac tacttacatc agctaccatt gtatgcagag gcgaagatgg
     2941 gtattctgtt tgaaggctgt aaagtttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca
     3001 cttttgcacc cgatgccagt aaggcatcta ccagtgcctt gccaataccg tctgtaccac
     3061 cggttactac caccacttta tctttaaaaa aggatgacat ataagattta atattatgta
     3121 atacaaacgt actccaaaag gtcaaaaaga aaaagaggct gtccttttta gacagcctct
     3181 ttgtgatccc gctgggactc gaacccaggg ccccaacatt aaaagtgttg tgctctacca
     3241 actgagctac gagatcaacc tttccttttt agggagtgca aaaatacggt ctgcaacgtt
     3301 ttgcgccaaa actttttcag gtaattatca acatcttttt tgaaaaatca gtcaggagag
     3361 aactgaccgc cttttgtttc atcatttaaa atgtccatta gtttgccaaa catttctatg
     3421 tattgcgata aacggccatg ctgtgtttca atgttgatat atcgtttgtt cttttcaccg
     3481 tagaatacac tcagggagcc gtctttcttt gcccgttcat tgtgctgcca gacgctgtta
     3541 tatccgcagt ctgccatttt tttgtacagc atgctgtcgg tagtaagtac aatatcatca
     3601 atgtcctggt tttcgttggc ataaacttca cgggcatcct tttccctttc attgcccggc
     3661 aaatagcttt ttatagaata agcattttct gtattgttat gaagtgccac aacacaaata
     3721 gtttcttccg ggataagatt cagtatacgg gtcgcaaatt tttcaatttc ttcaattgca
     3781 taaggatcag ctgtactgtt ttctcttaaa gtttgttcaa taccaacccg ggaaaaaatc
     3841 cggttggggt cgaatgcata gtttctgcct cttaagcgga aacgaatgac ccgctgcctg
     3901 ctgttatcta acttaataag taagccgcct ttttgttcca gcactgatct tgccgcttgt
     3961 actgacgtaa actcattatc gtgaaggttg atgcaaacaa tttctgatga attaccatac
     4021 tggctgatga gcagaggaat agttttatcg cccaatttgt aaagaattgt tttttcggcc
     4081 ggaatataaa aggcattact tgccaggtta acctcgttga caggctgggc ctgaagacgg
     4141 cctatgagca ataatgtata tatagaaagt aatagtcgca attattgtgt ttccctaaag
     4201 ataatttgct aggaggggga gggcataccg gtaaagcggt atttttcaac aatcaggcaa
     4261 gttcctcttt cattttctgc atcacatctt tttccagcat aagttgagac agttttatca
     4321 tgttcataaa cgctttggct ccagaaatac catggccaat gataactggt ttcgctacac
     4381 ctaaaactgg tgttcctcca tatatttcaa agttgaaccg gtcgaagtat tcgtcatcta
     4441 tcttttttct ttgaccgatt tcgtataaag attcagccaa tttaagtatg atattgccgg
     4501 taaaaccttc gcagaccatt acatcggctt tatccatcag tacatcacgg ccctcaatat
     4561 tgccaatgaa gtgaatgtgt ttattttctt ttaacagggg ataggtagcc tgtgccagca
     4621 aatttccttt tccttcctct tcacccacat tgatcaggcc tactttgggt ttttcaattc
     4681 ccaataccag ttgagcatat acggacccca taatggcaaa ctggttcagt tgttcaggtt
     4741 tgcagtcagc gttcaatcct acatccaata acaatccggt gccgccattt tcctttggaa
     4801 tgatggttga gatggttggc ctaatcaccc cttccagcgc ttttatgcta aataatgtgc
     4861 ccaccagcat ggcgcctgta ttaccagcgc taataaaagc atccatctta ccggatgcca
     4921 gcaggtgaaa gccaatggca atagatgaat tttgtttttc cctaagggct ttggtagggt
     4981 gttcatgcat atcgatgatt tcttctgcat gaacaatagt gatatgatct gatgggacat
     5041 catgctcggt cagcaatttt tgaatagctg gttcatcacc aattaatagg agatttaccg
     5101 gcttatcatt acccgataac cagtatttta ctccttttac tgcttccatc ggggcataat
     5161 cgccccccat catatcaagg ccgatcgtca taaaattcta aaatagcaaa ttccaaatcc
     5221 ctgcagaatt aaaagatccg ccggatttgg aatttgaaca gtattcgttg aatttatttg
     5281 ttaaccggag atttttctgc taccactttt cctttgtagt agagctttcc ctcactaaca
     5341 tgtgcacggt gacgcaggtg tgtttcacct gttgtgctgt cggtcgataa tgttgcagaa
     5401 gtagccttat aatgcgttct gcgctttgca cttctttgct gactatgtct gcgtttcgga
     5461 tttggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta atacgttaat ctaaatcttt
     5521 aaacttttct aaccccttcc agatggggtt ttctttgctt tccttttcat ttggctccat
     5581 ttttttcaat acatccagcg ctgcagggtt acaatgaggg ccatccattt tttcaaattc
     5641 acaaaccttt tgcattggaa tggtcaggtt aatgaattcg tagatccagt tggctacatc
     5701 caggtggctt tcgccgcggc tgatataata cacatccgga tcatcttcct ggtcattcat
     5761 tagttctggt tcttccacca tctttaccgt gatgttgaac tcatcccata attccagcgg
     5821 caggttattg ttgcaccggt cacaggttac ttccagggtg ccaccaattt caaatttgag
     5881 catcatgaag ctgctttttt tatccagcag cagcctgata ttagctttgc agttgtggaa
     5941 atcctgttgt tgaaaaggct caaagaactt atcgtcaatc gaatagttgt actcatgaac
     6001 accgggcttt aatcccacaa aagcaatctc aaactcccgg cgacggctca tttgtcaact
     6061 tttaaagtcc cgactttttc gggagtgcaa aggtagggta aaatacataa acagaagtgt
     6121 tgaatttgag attttttatc gaaaattggg tactttagcc gccattctta caccctaata
     6181 actaatcact tgcagcaatt tcttacccga ttgttcgccc gactgatgaa aagctggagc
     6241 tgggtagtcc ttattatctt tacaatcctg atcaaatggg catcctggta tcccggctgg
     6301 gtagaaagaa actacagcta cggtatttac ccgcttattt cgaaaataca gcggatttta
     6361 tttggctgga taccttttag tgtgggggat attttttatg ggttccttgt attggttatt
     6421 attttccgaa cctttaaatt tttcaaatac ctttttcagc gccggttgac aaggcagtat
     6481 ttcgttatcg ccatgcagca gttcatcttc tttgtgctgt ttgtgtatgt ttttttcaac
     6541 cttttannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnattatgct gcatttgtaa ccccggcaca gcgggattca tttaaccgta aaagggacct
     6721 gttcgccagt gcccacaagg cttacgaatc agccgcatcc aaccataaat tccttcaata
     6781 cagttcccgg tctgtgaaac cctcgctttt tagttacctg ggcaactatc ttggattcca
     6841 gggatactac aacccgtttt ccggagaggg acaggttaat accacaatac caaggttcat
     6901 ggagccattt gttacctcac atgaaatcgc ccatcagctg ggctatgcca aagaaaacga
     6961 agcaaatttt gcagcttatc ttgcctgccg ctcatacgaa aacaatgctt ttcgttattc
     7021 gatgtattat gacatgtata actatgcaat cgtggaagtt tttaggcggg atacaaccct
     7081 gggaaaatta atccaggaac tagcccatcc gcaggtaaaa aaagatcaaa aggaattcag
     7141 agatttctac aaaaaataca acaatcccat tgaagatctt gtgatgtggg gctacgggca
     7201 ttttcttaag gccaacaatc agcctgccgg caaaagaagt tataatgaag tagtggcctg
     7261 gctgattgca tattacaaga aatacggcat tgaagcgctt taaacatttc aaagcccaat
     7321 ttgttattgt ttaatgagca atattgtaaa gggaatagta gctattgcaa taccgttgct
     7381 ggttggagga gcaagcggat tctttactat taccggcgtt gacagctggt atcaaactat
     7441 taataaacct tcatggaacc cgcccaactg ggtatttggc ccggtgtgga ctactttata
     7501 tataatgatg ggcattgctc tttttcttgt gtggaaagaa gataccagtg aagagcttaa
     7561 aaaaattgct atcgccttat tttccgttca gctgattttg aattttttct ggtcttttat
     7621 ctttttcaac ctgcagcagc cgggttgggc gttggttgaa ataatagcta tgtggttttt
     7681 tattttgctg accatttttg cttttgtgca ggtgaataaa acggcggcct ggttgctggt
     7741 gc
//
LOCUS       DAORLS010000140         7737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5117_length_7737_cov_1.782218, whole genome shotgun sequence.
ACCESSION   DAORLS010000140 DAORLS010000000
VERSION     DAORLS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5117_length_7737_cov_1.782218"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /gene="rpsM"
                     /locus_tag="PK977_11220"
     CDS             <1..181
                     /gene="rpsM"
                     /locus_tag="PK977_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRF18734.1"
                     /translation="GQLRSEVQMSIKRLLDIACYRGLRHRKGLPVRGQRTKTNSRTRK
                     GKRKTVAGKKKAAKK"
     gene            358..768
                     /gene="rpsK"
                     /locus_tag="PK977_11225"
     CDS             358..768
                     /gene="rpsK"
                     /locus_tag="PK977_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRF18735.1"
                     /translation="MAKAQQQAQSAKAAAKKRVVKVDAYGDAHISATFNNIIISLTNK
                     TGQVISWSSAGKMGFKGSKKNTPYAAQMAASDAAKTAFDAGLKRVDVYVKGPGAGRES
                     AIRSISQSGIEVVMIKDVTPLPHNGCRPPKKRRV"
     gene            908..1513
                     /gene="rpsD"
                     /locus_tag="PK977_11230"
                     /pseudo
     CDS             908..1513
                     /gene="rpsD"
                     /locus_tag="PK977_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
     gene            1575..2576
                     /locus_tag="PK977_11235"
     CDS             1575..2576
                     /locus_tag="PK977_11235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRF18736.1"
                     /translation="MAILNFQKPDKIILQKATDFEAQFEFRPLEPGYGVTIGNALRRV
                     LLSSLEGYAIIGVKIEGVDHEFATMKGISEDVVEIFLNLKQVRFKKVIDHEVANEKII
                     LSIKNRTEFTAGMIGEGTHSFQIMNPDLLICTLDSSARMDIELTIGKGRGYVPAEDNK
                     PKDAPMGYIPIDAIFTPIKNVKYTIENTRVEQRTDFEKLIMEVATDGTIHPEEAVKQA
                     SRILIQHLMIITDENITFDNKEEKKEDLVDEQTLQLRKVLKTPLEDLDLSVRAFNCLK
                     AAKINSLSELVQYEQEDLMKFRNFGQKSLAEIEQVLTERGLHFGMDLVKLGIDLSEF"
     gene            2668..3162
                     /gene="rplQ"
                     /locus_tag="PK977_11240"
     CDS             2668..3162
                     /gene="rplQ"
                     /locus_tag="PK977_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRF18737.1"
                     /translation="MRHGDKVKNLGRKKAHRDALLSNLACQLIQHKRIVTTTAKAKAL
                     RGYVEPLITKGKDNTTHQRRVVFSYLQDKEAVKELFGTVAEKVAGRPGGYTRIIKLGF
                     RPGDNADTAMIELVDFNEIYGKGKGEAKAPAKKTRRSGGAKKKAEATDKTETPATTEE
                     KAAE"
     gene            3304..>3629
                     /locus_tag="PK977_11245"
     CDS             3304..>3629
                     /locus_tag="PK977_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRF18738.1"
                     /translation="MDHQSGRALQQFKSEVGIRFQLYNSLFTSLPFHRIEKTGILLSL
                     LSNNCEEEYKRKLSPADIIDDFFTRHTVYTKTEDRNDLLFRFVQYIERQVVLFDALED
                     AAFKYV"
     assembly_gap    3630..3677
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3678..>3896
                     /locus_tag="PK977_11250"
     CDS             <3678..>3896
                     /locus_tag="PK977_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18739.1"
                     /translation="KENELALKLNDFAIRLVLTAHPTQFYPGSVLGIINDLAKALADN
                     NTNLVNTYLQQLGKTPFFKKKKPTPYDEA"
     assembly_gap    3897..3910
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3911..>4210
                     /locus_tag="PK977_11255"
     CDS             <3911..>4210
                     /locus_tag="PK977_11255"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRF18740.1"
                     /translation="EAVSLIWYLENVFYPAAGKIISSLKNQFPDAVSKENSIIKMGFW
                     PGGDRDGNPFVTSEITLQVANALRGAIVKCYYMDVRKLRRRLTFEGVETLLQQLE"
     assembly_gap    4211..4280
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4281..>5317
                     /locus_tag="PK977_11260"
     CDS             <4281..>5317
                     /locus_tag="PK977_11260"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRF18741.1"
                     /translation="TLYRIKEVIIHQHNGLFLHLVNNLISKIELFGLHFASLDIRQDS
                     SVHGKLLDELSSQQSVLPLAYRDLAETEKMELLVSINSVIDISPVNDPVLQDTLLTIR
                     SIKEIQQFNGTEGCSHYIISQCNSALNAIEVYGLFLLSGWKPEEMNIDIVPLFETISD
                     LNNAATVMQSLYELPAYRQHLERRGKRQTIMLGFSDGTKDGGYLMANWSIYKAKEELT
                     RVSRKYNIDVIFFDGRGGPPARGGGKTHKFYASMGKNISNREIQLTIQGQTVSSNFGT
                     VDAAQFNIEQLLHAGITNDLFSTKETTLSNSEENLLQELSVAGFDAYKGLRDHPYLAD
                     YLLQVSPLRFY"
     assembly_gap    5318..5331
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5363..>5669
                     /locus_tag="PK977_11265"
     CDS             5363..>5669
                     /locus_tag="PK977_11265"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRF18742.1"
                     /translation="MSGLSLKDLRAIPFAGSWSQLKQNVTGYYGVGTALQQMEKKGKL
                     PELQKLYHQSLFFRTLLDNCEMAMMKSFFPLTAYLAGHKQFGELYQMIHDEYELTKQY
                     "
     assembly_gap    5670..5709
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5710..5902
                     /locus_tag="PK977_11270"
     CDS             <5710..5902
                     /locus_tag="PK977_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18743.1"
                     /translation="PVEQLSIQMREKIVLPLVTIQQYAMTRIREMEEQSAPVELRETF
                     EKLAMRCSFGIINAGRNSA"
     gene            complement(5918..7237)
                     /locus_tag="PK977_11275"
     CDS             complement(5918..7237)
                     /locus_tag="PK977_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRF18744.1"
                     /translation="MKKLTAVIPFALFSLFALLALFAPLYPDFDGTGKYNNTDIGWML
                     IASALVLLMTPGLSFFYGGMVSKKNVISTMLQSFIATGLITIFWVVIGFSLAFGSSFK
                     GIIGNPADFFLFKGINSQGPWSLAPTIPLLLFALFQLKFAIITPALVVGGTAERIRFT
                     SYILFMVLFSIFIYSPLAHWTWHPEGILFKMGVLDFAGGTVVHISAGCAALMGAIVLK
                     RRKTHILKEEVKPARIPYVLLGTGLLWFGWFGFNAGSALAVNSLAVSAFGTTNIASAA
                     AGITWIFFDVLRGQKPSALGFCIGAVVGLVAITPAAGFVGLPQSIFVGFFAAIVSNVA
                     VHWKGKTTIDDTLDVFPCHGLGGITGMILTGVFASKKINPAGNDGLFYGNTDFFLTQL
                     YGCLIVVAFATIMSFIIFKVVNFIHPLRVSDEEEEKGLDITQHNEKL"
     gene            complement(7257..>7737)
                     /locus_tag="PK977_11280"
     CDS             complement(7257..>7737)
                     /locus_tag="PK977_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18745.1"
                     /translation="SYPMLLKKTSSHEEGAARQWAVATKEFIGDTNGNLKALKIVDLE
                     WKVVDGRPASFVEIAGSERKIPCELALLAMGFVHPQHADLINDLGIDLDERGNVKATE
                     KDFRTNISKVFTAGDMRRGQSLVVWAISEGRECAKAVDEYLVGHSVLESKNERLAFI"
BASE COUNT         2360 a         1548 c         1693 g         1950 t
ORIGIN      
        1 aggtcagctt cgttcagaag tgcagatgag cattaagcgt ttgctggata tagcttgcta
       61 ccgtggtctt cgtcatcgta aaggtctgcc ggttcgtggg cagcgtacca agactaacag
      121 ccgcaccaga aaaggtaagc gtaaaacagt tgccggaaag aaaaaagcag ctaagaaata
      181 attcttcgcc tttggctcag aatggcaaat tgaattgtca tcctgagcaa agtgaaggag
      241 acttacattt tatgaatcag cgccggatag cttcgtaagt cagcaggagg gttgattcag
      301 gctttgggaa accaaagctt acattttaaa aataaagact acctcagaac atcaattatg
      361 gcaaaagcac aacagcaggc ccaaagcgca aaagcagcgg ccaagaaaag agtggtgaaa
      421 gtggatgctt atggagatgc ccacatttca gctacattca acaacattat catcagcctt
      481 acgaacaaaa ccggccaggt tatctcctgg agcagtgcag gtaaaatggg ttttaagggt
      541 tctaagaaga acacaccata tgctgcgcag atggctgctt ctgatgctgc aaaaacagca
      601 ttcgatgctg gtttgaaaag ggtagatgtg tatgtaaaag gacccggtgc cggtcgtgag
      661 agtgccatcc gttcaatttc ccagagtgga attgaagtag taatgatcaa ggatgtgacg
      721 cctttgccgc acaatggttg ccgtcctcct aagaaaagaa gagtataaga aagcttatag
      781 ctcattgcta tcagctcaga catattgaag catagtgctt tcatttaatt aaagggtgac
      841 tgattaattt ttaccgcttt caacgatcag ttcaccggtt tttaaaaagc ggaaatgaat
      901 aaaaactatg gcaaggtaca acggtccaaa gaccaagatt tcccgcattt ttggtgagcc
      961 tatcttaggc aatggcaagt ggttgggtaa aaacagcaac cctcccggtc agcacggtga
     1021 aaagcgtaag cgtaagacat gaggtgaata tgcttgacag ctccgtgaaa aacaaaaagc
     1081 taaatactct tacggagtat tggaaaaaca attccgcaaa acattcgaag aagcagcccg
     1141 ccgtaaaggt gtaacaggtg agaacctgat caaactgctg gaagcccgcc tggataacac
     1201 tgttttccgt atgggtatcg ctcccagccg tccggccgcc cgccagttag taagccacaa
     1261 gcatgtggaa gtgaacggcg aagtagtgaa tgttccttct taccagctta agccgggtga
     1321 tgtgatcact atcaaggaaa gcagcaaagg aagaacatcc attaccagtg ttgttcgtcc
     1381 aaagaacccc aaattcagct ggctcgactg gaatgaaaca gaactaaaag gtactttcat
     1441 tacctatcct gaaagggaaa gtgttccgga gaatattaaa gagcagctga tcgtggaatt
     1501 gtattctaag taagcttgaa tggctgtaaa tgttcagcca accaaaattg taaatcctaa
     1561 atctaaattt caatatggca attttaaatt ttcagaagcc tgataaaatc attctgcaga
     1621 aagcaactga ttttgaagct cagtttgagt ttcgtccgtt ggaacccggc tacggtgtaa
     1681 ccattggtaa tgcacttcgc cgtgtgttgc tcagttcact ggaaggctat gctattattg
     1741 gtgtgaaaat agaaggtgta gatcatgaat tcgcaaccat gaaaggtatc agcgaagatg
     1801 tggtggaaat tttcctgaac ctgaagcagg ttcgcttcaa aaaagtaatt gaccacgaag
     1861 tggccaatga aaaaatcatt ctttcaataa agaacagaac agagttcaca gccggtatga
     1921 tcggagaagg aacacattct ttccagatca tgaacccgga tctcctgatc tgtaccctgg
     1981 attcttctgc aagaatggat attgaactta ctatcggtaa aggccgtggt tatgtacctg
     2041 ctgaagataa caaaccgaaa gatgcgccga tggggtacat tccgattgat gccatcttta
     2101 cgccgatcaa gaatgtgaag tacaccatcg agaatacccg tgtggagcaa agaacagact
     2161 ttgaaaaact gatcatggaa gtggctaccg atggtaccat ccacccggaa gaagcggtga
     2221 agcaggcttc aagaatcctg atccagcacc tgatgatcat cactgatgag aacatcactt
     2281 ttgacaataa ggaagaaaag aaagaagacc tggtggatga gcaaacttta caattgcgta
     2341 aagtattgaa gactccgctg gaagatcttg atctttctgt tcgtgccttc aattgcttaa
     2401 aggctgccaa gatcaactca ctgagtgaac tggtgcagta cgagcaggaa gacctgatga
     2461 aattccgcaa cttcggacag aaatcactgg ctgaaattga gcaggtgctg acagaaagag
     2521 gtctgcactt cggaatggac ctggtgaaac tgggtatcga tctgagcgaa ttctaattgt
     2581 atgcagtttg tcttattggc aaactgctat tttttataat gtaggctgta attcctgaca
     2641 cggtacagct gaataaaact aaacgtcatg cgtcacggag acaaagtaaa aaatctcggc
     2701 agaaagaaag cccacagaga tgcgttgtta agcaacctgg cctgccagct gatccagcac
     2761 aaaaggatcg ttactacgac cgccaaagca aaagctttga gagggtatgt tgagccattg
     2821 atcacaaaag gaaaggataa taccacacat cagcgtcgtg ttgtatttag ctatttgcag
     2881 gacaaagaag ccgtaaagga gttgttcggt actgtggctg aaaaagtggc cggtcgtccc
     2941 ggtggttata cccgcatcat taaattagga tttcgtcctg gtgataatgc tgatacagct
     3001 atgattgagc tggttgattt caacgagatc tatggcaagg gtaaaggcga agccaaagct
     3061 ccggccaaaa agacccgccg ttctggtggt gctaaaaaga aagcagaagc aactgataaa
     3121 acagaaactc ctgctacaac agaagaaaaa gctgctgaat aatagaagcc aacatctgtt
     3181 tgatataatg cccccggagt ttcgggggca ttttttattg gttgatctta tggcgaatta
     3241 ttgaataaat aaactatttt cactttactg cttgctgttt ttgaaataca ttaaattacg
     3301 aacatggacc atcaatccgg cagagcattg cagcagttta aaagcgaagt gggtattcgc
     3361 ttccagctat acaatagttt atttacctca ctgccttttc accgaattga aaaaacaggg
     3421 attttgcttt cgcttttgtc aaataattgc gaggaagagt ataaaagaaa actaagtcct
     3481 gccgatatta ttgatgactt ctttacccgg catactgttt atacaaagac agaggacagg
     3541 aatgatcttc tatttcgttt tgtacaatat atcgaaagac aggttgtgct ttttgatgca
     3601 ctggaggatg cggcttttaa gtatgtaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnaag gaaaacgagc tggcgctaaa gctaaatgat tttgccatca
     3721 ggctggtgct tacagctcat cctacccagt tttatcccgg atcagtgctg gggatcatca
     3781 atgacctggc aaaagcattg gctgataata acactaacct tgttaatacc tacttacaac
     3841 agctgggaaa aacacctttc tttaaaaaga aaaagcctac tccttatgat gaggctnnnn
     3901 nnnnnnnnnn atgaggctgt cagtcttatc tggtacctgg aaaatgtatt ttatccggcg
     3961 gcaggcaaaa tcatctcatc cttaaagaac cagttcccgg atgccgttag caaagaaaac
     4021 tccattatca aaatgggctt ctggcctggt ggcgacaggg atggtaaccc ttttgtaaca
     4081 agtgagataa cattacaggt ggccaatgca ctcaggggtg ccatcgtcaa gtgttattac
     4141 atggatgtcc gtaagttaag aagaaggctg acatttgaag gagtagaaac gttgctgcaa
     4201 cagcttgaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn acattgtaca ggataaaaga agtgatcatt caccagcata
     4321 atggtttgtt cctgcatctg gtgaacaacc tgatcagtaa aattgaatta ttcggtttgc
     4381 attttgcttc tctggacata cggcaggata gttcagtaca cggaaagtta ctcgatgaac
     4441 tttcatcaca gcagtcagta ttacctctgg cttatagaga tcttgctgaa actgaaaaaa
     4501 tggaattgct ggtttcaatt aattcggtaa ttgatattag tccggtaaat gatccggtgc
     4561 tgcaggacac attactgact attcgcagca taaaagaaat tcagcagttt aacggaaccg
     4621 aaggctgcag tcattacatt atcagccaat gcaacagtgc tttgaatgca atagaagtct
     4681 acgggttatt tcttttgtct ggctggaagc cagaggaaat gaacatagat atagtgccac
     4741 tttttgaaac catcagtgat cttaataatg ccgccaccgt gatgcagtca ttatacgaac
     4801 tgcctgcata ccgtcagcac ctggaacgaa ggggaaagcg ccaaacgata atgctcggtt
     4861 tttcggatgg taccaaagac ggcggctacc tgatggctaa ctggagcatc tataaagcca
     4921 aggaggaact gacccgggta tcaaggaaat ataatattga tgttatattc ttcgatggtc
     4981 gcggaggccc gcctgcaaga gggggaggga agacgcataa attttatgca tcaatgggaa
     5041 agaatatttc caacagggag atacaactga ctatacaagg acagaccgtt agttccaact
     5101 tcggtacagt ggatgctgca caattcaaca ttgagcaatt actgcatgcg gggatcacta
     5161 acgatttgtt ttctaccaaa gagactacac tttccaatag tgaggagaat ctattgcagg
     5221 aactgtctgt tgctggtttt gatgcgtaca aagggctcag ggatcatccc taccttgcag
     5281 attatttatt acaggttagc cccttgcgat tttacagnnn nnnnnnnnnn ntattggaag
     5341 ccgaccggca aaaagaggaa ccttgtcagg attatcttta aaagacctca gggcaattcc
     5401 ttttgccggt tcatggagcc agctgaaaca aaacgtaacg ggttattatg gcgtaggtac
     5461 tgcattacaa caaatggaaa agaagggaaa attgccggag ctgcaaaaat tatatcacca
     5521 gtcattgttt ttccggacac tgctggataa ttgtgagatg gccatgatga aatcattttt
     5581 cccgctgaca gcgtatctcg ccgggcataa gcaatttgga gagctatatc aaatgatcca
     5641 tgatgaatat gaactgacca aacagtattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnt ccagttgagc agttgtcgat acaaatgcgg gaaaagattg tgttaccgct
     5761 ggtaaccatt cagcaatatg ctatgacccg tatcagagaa atggaagaac agtcggcacc
     5821 tgttgaatta cgggagacct ttgaaaaact ggctatgcga tgttctttcg gtattatcaa
     5881 tgccggaagg aattcggcat aatgattaat ttcattctta tagcttttca ttgtgctgtg
     5941 taatgtctaa gcctttttct tcttcctcat cagacactct taaaggatga atgaagttta
     6001 ccactttgaa aatgataaaa ctcataattg tagcaaacgc caccacaata agacaaccat
     6061 acagttgtgt caggaaaaaa tcagtatttc catagaataa accgtcatta ccggcagggt
     6121 taattttttt cgatgcaaaa accccggtga gaatcatgcc tgttatgcct cctaatccat
     6181 gacaagggaa tacatccaat gtatcatcaa tggttgtttt tcccttccaa tgcacggcaa
     6241 cgttagagac aatggctgca aagaagccta caaatatact ttgcggcaaa ccaacaaaac
     6301 ctgctgccgg tgttatagcc actaatccca ctacagcgcc aatacaaaaa ccaagagcag
     6361 agggtttctg acctctcaat acatcaaaga agatccaggt aatacctgca gcagcagatg
     6421 caatattggt ggtgccaaaa gctgacacag ccaggctgtt tactgccaag gctgaaccgg
     6481 cattaaaacc aaaccagcca aaccataaaa gaccggtgcc taacaaaacg taaggaatac
     6541 gggcaggctt tacctcttct tttaagatat gcgtctttct tcttttcaac acaatagcac
     6601 ccataagtgc tgcacaaccg gcggatatat gtactactgt gccacctgcg aaatccaata
     6661 ctcccatttt aaaaagtatt ccttccgggt gccaggtcca gtgagcaagc ggtgagtaaa
     6721 tgaaaatact gaacagcacc atgaatagta tgtaagaagt aaagcggatt ctttctgcag
     6781 tgccgccaac cacaagggca ggagtgatga tggcaaattt caactggaat aaagcaaata
     6841 aaagcagggg aatagtgggt gcaagactcc atgggccttg tgaattgatt cctttaaaaa
     6901 gaaaaaaatc cgcaggattg ccaattattc ccttaaagga acttccgaat gcgaggctga
     6961 acccgataac tacccaaaat atggttataa gaccagttgc aataaagctt tgcaacatgg
     7021 tggagattac attcttttta ctgaccatgc cgccatagaa gaatgacaga cctggagtca
     7081 ttagtaaaac aagtgcagag gcaatcagca tccagccaat atcagtattg ttgtatttcc
     7141 cggttccgtc aaagtcggga tacaggggag caaaaagagc taagagggca aacaggctaa
     7201 ataatgcaaa tggaataaca gcagttagtt ttttcataaa gtgtaaacta ttttatttat
     7261 ataaatgcaa gtctttcatt tttggactcc agtactgaat ggccaaccaa atactcatct
     7321 actgcctttg cacattcccg tccctcactg atggcccaaa ccactaggct ttgcccgcgg
     7381 cgcatatccc cggctgtaaa aactttagaa atatttgttc ggaaatcttt ttcagtggct
     7441 ttcacatttc ctctttcatc caggtcaatt ccaagatcat tgataaggtc tgcatgttgc
     7501 ggatgaacaa agcccatggc cagtaaggca agttcacagg gtatctttct ttcactgccc
     7561 gctatttcaa caaaactcgc aggtcgccca tcgacgacct tccattcaag gtcaacaatt
     7621 ttgagagcct tcaggtttcc gtttgtatct ccaatgaact cttttgtagc aactgcccat
     7681 tgtcttgctg ctccttcttc atgtgagctg gtttttttta agagcatggg ataagaa
//
LOCUS       DAORLS010000141         7727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5127_length_7727_cov_1.940824, whole genome shotgun sequence.
ACCESSION   DAORLS010000141 DAORLS010000000
VERSION     DAORLS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5127_length_7727_cov_1.940824"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PK977_11285"
     CDS             complement(<1..983)
                     /locus_tag="PK977_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF18746.1"
                     /translation="MRFIPFVLVFAALFLVSCNKKKKEAANAKPAAGAKAPPMKVDGY
                     LVKTEPFQENIEVPGTIVANEVAEIHPEVSGRIVQLNVAEGKYVSKGTLLAKLYDGDL
                     RAQLNKLQIQLALAEKTEERQAQLLKIQGISQQDYDISLLQVNSLKADIGILQTSIAK
                     TVIRAPFSGKLGLKAISPGAYVTPATVIAVINQTDQLKLDFSIPEKYTSQIKIGQLVT
                     FTIEGSKKQLGAKIIATESNVMENTRSLTVRAAVMGKDEALLPGAFAKVQLSFDPDPN
                     AILVPTQAVLPQARGKKVILYKGGTAIFADVTTGVRDSARVQILEGLNAGDT"
     gene            complement(1118..>1389)
                     /locus_tag="PK977_11290"
     CDS             complement(1118..>1389)
                     /locus_tag="PK977_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRF18747.1"
                     /translation="VILGYPLGNTDAILPSIMVNIIGAEGFSGTVKYEGLEETLRIEN
                     AFVHLYGKKQTKPGRKMGHVTILSKEKQELLHRSNKVKQLLQVKA"
     assembly_gap    1390..1404
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1405..2241)
                     /locus_tag="PK977_11295"
     CDS             complement(<1405..2241)
                     /locus_tag="PK977_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF18748.1"
                     /translation="MPKSGILGGGQLGRMLLQAAANYPVETYVLENDAECPAAHLCHH
                     FTKGDIKNFDAVYNFGKGLDAITIEIENVNVEALERLENEGVKVYPKPSVLRIIKNKI
                     LQKEFYRNHNIPSAEFIITNNLVELQEAENFLPAVHKIGEGGYDGRGVQLLRSKQDIA
                     KGFDAPSVLEKMVPVYKEIAQMVAVNEKGETALYPPVEMLFDPVLNLLDYQLCPAEID
                     MKTLWKVEAISLTVARSLNSPGIFAVEMFIDKNGEVLVNETAPRVHNSGHHTIEAHYS
                     SQF"
     gene            2463..>2624
                     /locus_tag="PK977_11300"
     CDS             2463..>2624
                     /locus_tag="PK977_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRF18749.1"
                     /translation="MNTVTATTDKKQTIETDFLPLEGTDYVEFYIGNAKQAAHYYMSA
                     FGFQALAYAG"
     assembly_gap    2625..2678
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2679..3617
                     /gene="hppD"
                     /locus_tag="PK977_11305"
     CDS             <2679..3617
                     /gene="hppD"
                     /locus_tag="PK977_11305"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRF18750.1"
                     /translation="VRQHKLTFVLTTPLRADNPIADHIYKHGDGVKALSLRVQDAARS
                     WEETTKRGAKSYMEPTRLTDEHGEVVMSGIHTYGDTVHLFIERKNYTGAFMPGYRPWS
                     NPYFKPSDTGLLYVDHCVGNVGWNQMNPWVKFYEEVMGFKNILSFDDNDISTEYSALM
                     SKVMSSGNGYVKFPINEPAEGKKKSQVEEYLDFYNGEGCQHVAMATNDIVKTVTALRD
                     RGVEFLRVPNTYYDDLLDRVGHIDEDLEPLKELGILVDRDDEGYLLQLFSKPVEDRPT
                     LFFEIIQRKGAKSFGKGNFKALFEALEREQDLRGNL"
     gene            3756..4949
                     /locus_tag="PK977_11310"
     CDS             3756..4949
                     /locus_tag="PK977_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRF18751.1"
                     /translation="MKSLLTLITLTFFSAAIVAQPPSSKSGGPSYSFVDYQRAFPRPG
                     EAMQRKLDTLQKQFEAKKLQWPAKYMYIRSFKYDSQLEVWVKNEINAPFQLFKAYKVC
                     ALAGTLGPKRMEGDYQVPEGFYYINEFNPKSNYYLSLGINYPNASDKILSDSLRPGSA
                     IYIHGSCVTVGCIPITDQQIDELYILAAHAKDQGQDFIPVHIFPVRFDKEKSVSYLEG
                     LTKDDPVLKKFAERMEDAFDYFEKYKQLPVVMVGEKGEYIINEVPPKKAKLIIEDKQP
                     VKKPPMARRTRNIIGLADAVHQWPQFPGGTDAFMKYLEQTGREAGSFLPKGVKKAYIQ
                     LEFIIDKDGVPVNFKILKSIKDADELEDELISRLENMGTWKPAILHDKPVAKKMIQTI
                     TIETP"
     gene            5099..>5333
                     /locus_tag="PK977_11315"
     CDS             5099..>5333
                     /locus_tag="PK977_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18752.1"
                     /translation="MRTIKIFATTLLFTAFVIAGCQKNAKELTGTDSNSVEKQVNQNG
                     PCNSNAYQVLLESKTFSNGNWVWIWSVKNPNPGN"
     assembly_gap    5334..5358
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5359..5709
                     /locus_tag="PK977_11320"
     CDS             <5359..5709
                     /locus_tag="PK977_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18753.1"
                     /translation="QDLSHWGMQFGTCFNWADVVSAAYSSNGNSWVSFNPSYSVDPSQ
                     SCLTTPVLKFDFGTSGSNKSYYRLVLSRDYVVNQSAIAYYKSGRRMPCCTFSFSGVGC
                     NNTIPTRQAQVLAN"
     gene            complement(5706..>6025)
                     /locus_tag="PK977_11325"
     CDS             complement(5706..>6025)
                     /locus_tag="PK977_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRF18754.1"
                     /translation="EQHVIHLYHLMEKMHIKPNLTQNGAISFLCVIDEWPEKIEKLAL
                     EASSFLDVQVTKGLSLLTIRHYNKEIFEYLTQKKTILLRQQTPETIQVLLQQNRMDEI
                     QNN"
     assembly_gap    6026..6102
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6103..>6378)
                     /locus_tag="PK977_11330"
     CDS             complement(<6103..>6378)
                     /locus_tag="PK977_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRF18755.1"
                     /translation="AAVFANMLDAESQTIWKDVESVMNADPKQFPGAVAIEELNYNEV
                     IEMAYYGAQVIHPKTIKPLQNKGIPLYVKCFLNTSLKGPIINDRPVHG"
     assembly_gap    6379..6587
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6588..6994)
                     /locus_tag="PK977_11335"
     CDS             complement(<6588..6994)
                     /locus_tag="PK977_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRF18756.1"
                     /translation="MKVFKFGGASVNSVERIKNMAEIMRQYNGEKLMVIVSAMGKTTN
                     ALEKVTKAFCDGHKDEALFLFEQLKQQHLTTAKYLLVTNYLSCEVQLKDFFTEVEWLL
                     HDKPVREYDYYYDQVVCAGELFSTSIVSHYLNE"
     gene            7115..>7727
                     /locus_tag="PK977_11340"
     CDS             7115..>7727
                     /locus_tag="PK977_11340"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRF18757.1"
                     /translation="MNVNRRVQTLDEFTIQQLRDFPQATGELSHLLRNIGLAGKRVHV
                     EVNKAGLVDILGDAGTINVQGEEVKKLDVYANDQFTRVLQHGIACAGIASEELDDFVP
                     FDDAKSVKSKYVCLFDPLDGSSNIDVNVSIGTIFSIYRRVTPIGTVVTKEDFLQPGNQ
                     QVAAGYIVYSSSTMLVYATRRGVNGFTLDPSIGEWTLSHPDIKC"
BASE COUNT         2083 a         1463 c         1561 g         2240 t
ORIGIN      
        1 gtgtcgccgg catttaatcc ttccagtatt tgtactcttg ctgagtcccg gacacctgtt
       61 gtaacatcgg caaaaatggc ggtaccgcct ttgtacagta ttaccttttt ccctcttgcc
      121 tgcggtagca cggcttgtgt gggaaccaat attgcattag gatcagggtc aaaacttagc
      181 tgcaccttgg caaaagcgcc gggcaaaagg gcttcatctt tacccattac ggcagctctt
      241 actgtcaggc ttctggtatt ttccattacg ttcgattcgg tagcaataat ttttgctcct
      301 aattgcttct tagaaccttc gattgtgaat gttaccagct gaccgatttt gatctggctg
      361 gtatatttct caggaatact gaaatcaagt tttagctggt cagtttggtt aatcacggca
      421 ataacagtag ccggtgtaac ataagcgccg gggctgatgg cttttaatcc caatttaccg
      481 ctaaaagggg ccctgatgac agtcttagcg atgcttgttt gtaatattcc aatatctgct
      541 ttaaggctgt ttacctgtaa aaggctgata tcataatcct gctggctgat accctgaatt
      601 tttagtagct gcgcctgcct ttcttctgtt ttttcagcga gggccagttg tatctgtagt
      661 ttattgagct gggcccgcag gtccccgtca tacagtttgg ccagcaaagt ccctttgctt
      721 acatattttc cttcagcaac attcaactgt actattctgc cggatacttc ggggtggatt
      781 tcagctactt cattggccac aatagttccg ggtacttcaa tattttcctg gaaaggctct
      841 gtttttacga ggtatccatc tactttcatg ggaggtgcct tggcgcctgc ggctggtttt
      901 gcattcgctg cctctttttt ctttttgtta caggatacca ggaataaagc agcaaaaact
      961 aaaacaaaag gtataaatct catttggttt gggtgtaatc gtaaaaaagt aaataaaggt
     1021 aacggattat cagtagcatt tagacgggga aaggagcaga taccttcacc tgaatcccca
     1081 tgcattataa gtggtttata tgttggataa gaggaactta tgccttgact tgtaataatt
     1141 gtttcacctt attggagcgg tgcagcagtt cctgtttttc tttactaagg atggtcacat
     1201 gtcccatctt tcgtccgggc ttagtttgct ttttaccata aagatgaaca aaggcatttt
     1261 caatccttaa tgtctcttcc aacccttcat attttacggt tccgctgaaa ccttccgccc
     1321 caataatatt taccataata gatggcagaa tagcatctgt attacccaaa ggatagccca
     1381 atattacccn nnnnnnnnnn nnnnaaactg cgaagagtaa tgagcctcta tggtatgatg
     1441 cccgctgttg tgcaccctgg gtgctgtttc gttgaccagt acttccccat ttttgtcaat
     1501 aaacatttcc actgcaaaaa tgccgggtga attcaggctt ctggcaacag taagtgatat
     1561 tgcttctact ttccatagag ttttcatatc aatctcggcg gggcacaact gatagtcgag
     1621 caggttcaat accggatcaa ataacatttc caccggaggg tacagcgctg tttcgccttt
     1681 ttcgttcacg gctaccatct gtgcaatttc cttataaaca ggcaccattt tttccagcac
     1741 agaaggggca tcaaatcctt tggcaatgtc ttgttttgat cttaagagtt gaacaccacg
     1801 accatcatat ccgccttcac cgattttatg tactgcgggg agaaaatttt cagcttcctg
     1861 taattctacc aggttgttcg taataatgaa ttctgcagat ggaatattat gattgcggta
     1921 aaactctttc tgtaatattt tgttctttat aattcttaaa acagaaggtt taggatatac
     1981 tttaacaccc tcattttcca gccgttcaag cgcctcaaca tttacatttt caatttcgat
     2041 agttatggca tcaagacctt ttccaaaatt gtaaacagca tcaaaattct tgatatctcc
     2101 tttggtaaaa tggtgacaaa gatgggcagc cggacattct gcatcgttct ccagcacata
     2161 ggtttcaaca gggtaattgg cagctgcctg taataacata cggcctaatt gaccgcctcc
     2221 aagaattccg ctttttggca tggggcaaaa gtaattaaga agaggcagag gatgagtaat
     2281 tcttgtacaa tgatttttct aactttgacc catgactccg gattatcctc ataagttatt
     2341 ttctgacgaa agaagaggct tcaacctctt gatggtgaca ttagccatgg attcttcctg
     2401 ctgttaagcg ggaagtattt tgcttccctg tgtttatact ttcactttta aattaactaa
     2461 tcatgaatac agtaacagca actacggata aaaaacaaac tattgaaaca gattttcttc
     2521 cgctggaagg aaccgattat gtggaattct atataggaaa tgccaagcag gcagcccact
     2581 attatatgag tgcattcggt ttccaggcac ttgcttatgc cggtnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tcgtcagcac aaactgactt
     2701 ttgttttaac cactccatta cgggcagata acccaatagc tgatcatatc tataaacacg
     2761 gagatggtgt aaaggcatta tcactcaggg tacaggatgc tgccaggtca tgggaagaaa
     2821 cgactaaacg gggtgcaaaa agctacatgg aacctacaag actaactgat gaacatggtg
     2881 aggtggttat gagtggtatt catacttatg gcgatactgt acatcttttt attgaaagaa
     2941 aaaactatac tggtgcattt atgcccggtt accggccttg gagtaatcct tattttaaac
     3001 catcggatac aggtcttttg tatgtcgacc attgtgtggg taatgttggg tggaaccaga
     3061 tgaatccctg ggtaaaattt tacgaggagg taatgggctt taaaaatatc ctctcttttg
     3121 atgataatga tatttctacc gagtattcag cattgatgag taaagtgatg agcagtggca
     3181 atggatatgt gaagtttcct atcaatgaac cggcagaagg caaaaagaaa agccaggtag
     3241 aagaatacct tgatttttat aatggtgaag gctgccagca tgtggctatg gctaccaatg
     3301 atattgtaaa aacggtgact gcactccggg acaggggagt tgaattttta agagtaccca
     3361 atacatatta tgatgacctg ctcgacaggg taggacatat agatgaagac ctggagcctt
     3421 tgaaagagtt gggtatcctt gttgaccgtg atgatgaagg ttacttacta caattattca
     3481 gtaagccggt ggaagacagg ccaacccttt tctttgaaat cattcagcga aaaggagcca
     3541 aaagttttgg caaggggaac ttcaaggcgt tatttgaagc actggagaga gagcaggatc
     3601 tcaggggaaa cctataaacg aacaatgtat caatgaaaat gggtcaggag cagccgcttt
     3661 tgacctattt atctttagtt cttatcttta taaacagttt agcgaatcag cagaaataaa
     3721 atccgtttcc cgccgttaag ctaaaattca ggacaatgaa aagccttctt acgctaataa
     3781 cacttacttt tttctcagca gccattgtgg cgcaaccacc ttcgtctaaa agcggggggc
     3841 cctcttactc atttgttgat tatcagagag catttccccg gcccggcgag gccatgcagc
     3901 gaaaactgga taccttacaa aagcagtttg aagcaaagaa attgcaatgg cctgctaaat
     3961 acatgtatat ccgttctttc aagtatgaca gccaactgga agtctgggta aagaatgaaa
     4021 tcaatgcacc ctttcagctt ttcaaagcat ataaagtttg tgccctggcc gggacccttg
     4081 gccctaaaag aatggaaggt gattaccagg tgcctgaagg attttattat atcaatgaat
     4141 ttaaccccaa aagcaattat tatctctcac tcggtattaa ttatcccaat gcttctgata
     4201 agatactgag cgactcctta agacctggca gtgccattta tattcatggt agctgtgtta
     4261 ccgttggctg catacccatc acggaccagc agatagacga gctatatatt ctggcggcgc
     4321 atgcaaagga tcagggccag gattttatac ctgttcatat tttccccgtc cgctttgata
     4381 aagaaaaaag tgttagctac ctcgaagggc tcaccaagga tgaccctgta cttaaaaaat
     4441 ttgctgaaag gatggaagat gcctttgact atttcgaaaa atacaagcaa ttgcctgtag
     4501 taatggtagg ggagaaaggc gaatatatca ttaatgaagt gcctcccaaa aaggcaaaac
     4561 tgatcattga agacaaacag ccggtgaaaa agcctcctat ggccagaaga acaagaaata
     4621 ttatcgggct ggctgatgcg gtacatcaat ggcctcaatt tcccggtgga acggatgctt
     4681 tcatgaagta cctggaacaa accggaagag aagccgggag ttttttaccc aaaggtgtaa
     4741 aaaaggcata catacaatta gagttcatca tagataaaga tggagtgcca gtaaatttta
     4801 aaattctcaa gagtatcaag gatgcggatg aactggaaga tgaacttatc agccgccttg
     4861 agaatatggg tacctggaag ccggcgatat tacatgataa acctgtagcc aagaagatga
     4921 tacagacaat aacaattgaa acaccctaat tggttaattg tgagttttag taattttcct
     4981 attttgattt gacttatcaa tcttttttat attttgtgaa taaatagttt attcaatcct
     5041 ttgaatttcc ggttgattaa taacaaaaca tcattattca ccttaatcgg aaatttacat
     5101 gagaacaatt aagatcttcg ccaccacttt actttttacg gcatttgtta ttgccggttg
     5161 tcagaaaaat gctaaagagt taaccggtac tgacagtaac tcggttgaaa aacaagttaa
     5221 tcagaacgga ccgtgcaaca gtaatgctta ccaggtttta ctggaaagca aaacgttttc
     5281 aaatggtaac tgggtatgga tatggagtgt aaaaaatccc aaccccggta atgnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnca ggacttgagt cattggggaa tgcagtttgg aacatgtttc
     5401 aactgggctg atgttgtttc tgcagcctat agctccaacg gaaatagctg ggtttctttt
     5461 aatccatcct attctgtgga cccgagccag tcttgcctta caacccccgt gttaaagttt
     5521 gattttggta catcaggcag caataagtca tattatcgcc tggtactaag ccgtgattat
     5581 gtagttaatc aatctgctat tgcgtattat aaatcaggaa gacgtatgcc ttgctgtacc
     5641 tttagtttta gtggtgttgg ttgcaacaat accattccaa caaggcaggc ccaggtatta
     5701 gctaattaat tgttttgtat ttcatccatc ctgttttgct gcaatagtac ctgaattgtt
     5761 tcaggtgttt gttggcgaag caggatggtt tttttttgcg tgaggtactc aaaaatttct
     5821 ttattataat gccggatggt taataaagat aaccctttgg taacctgtac atccaagaat
     5881 gaagaagcct ccaatgccag tttttctatt ttctccggcc attcatctat tacacaaagg
     5941 aaactgattg cgccgttctg tgttaggtta ggttttatat gcatcttttc catcaggtgg
     6001 taaagatgga ttacatgttg ttcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccatgaac gggtctatca
     6121 ttaattattg gtccctttaa ggaagtatta aggaaacatt ttacatataa cggtatgcct
     6181 ttgttttgta agggctttat tgtttttggg tgaatgacct gtgcaccata ataggccatt
     6241 tcaattactt cattgtaatt taattcctca attgcaactg cacccggaaa ttgttttggg
     6301 tctgcattca ttacactttc cacatctttc cagatggtct ggctttctgc gtccagcata
     6361 ttggcaaata cggctgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt tcgttgaggt
     6601 aatgacttac aatagaagtt gagaaaagtt caccggcaca aactacctgg tcataataat
     6661 agtcatactc ccttactggt ttatcgtgca gcagccattc tacttcggta aaaaagtctt
     6721 ttaattgtac ttcgcaggaa agatagttgg taactagcag gtacttcgca gtggtcaggt
     6781 gttgttgttt aagctgttca aacaaaaaga gggcttcatc tttatggcca tcacagaaag
     6841 cctttgtcac tttctccaat gcgttagtgg ttttgcccat ggcagaaaca atgaccatta
     6901 gtttttcacc gttatattgc cgcatgatct cggccatatt ttttatccgc tccacactgt
     6961 taacagaagc acctccaaat ttgaatactt tcatgtaaaa gccttttgtt gataagttgg
     7021 caaaattcgg gatatttctc ttctgacagc tatctctttc ttcttttcaa ctcctccaca
     7081 ataccttaca tttgccagag tcaaatcagc cattatgaat gtcaaccgga gagttcagac
     7141 tcttgatgaa tttactatac agcagctgag agatttccct caggctaccg gagaattgtc
     7201 tcatttgctg cgaaatatcg gccttgctgg caaaagggta catgtagagg tgaacaaagc
     7261 aggtctggtg gatattttag gagatgccgg aactatcaat gtgcaaggtg aggaagtaaa
     7321 aaaacttgat gtgtatgcta atgatcagtt tactcgggtg ttacaacatg gaatagcctg
     7381 tgccggtatc gccagtgagg aactggatga ttttgtacct tttgacgatg caaaaagtgt
     7441 aaaatcaaaa tacgtttgcc tgtttgatcc gctggatgga agcagcaata tagatgtcaa
     7501 tgtttcaatc ggcaccatct ttagtatcta tcggagagta accccgatag gaactgtagt
     7561 gacaaaagaa gattttttac agccggggaa ccagcaggta gctgcgggct atattgtgta
     7621 tagctcatcc acaatgctgg tatatgcaac gagaagagga gtgaatggtt ttacattaga
     7681 tccatccatt ggtgagtgga cacttagtca tccggatatt aagtgcc
//
LOCUS       DAORLS010000142         7716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5138_length_7716_cov_1.964626, whole genome shotgun sequence.
ACCESSION   DAORLS010000142 DAORLS010000000
VERSION     DAORLS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5138_length_7716_cov_1.964626"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..490)
                     /gene="iscU"
                     /locus_tag="PK977_11345"
     CDS             complement(92..490)
                     /gene="iscU"
                     /locus_tag="PK977_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRF18758.1"
                     /translation="MAYSEKVIDHYQNPKNVGTLDKSKNNVGTGLVGAPECGDVMRLQ
                     IEVDEATGVIKDAKFKTFGCGSAIASSSLATEWLKGKTVDEAMAIDNMDIVEELALPP
                     VKIHCSVLAEDAIKSAIADYKTKNTIPAEA"
     gene            complement(523..>810)
                     /locus_tag="PK977_11350"
     CDS             complement(523..>810)
                     /locus_tag="PK977_11350"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="catalyzes the removal of elemental sulfur from
                     cysteine to produce alanine; involved in NAD biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRF18759.1"
                     /translation="EGLLMGFNKNIALSSGSACTSASLEPSYVLKALGLGDDLAHSSL
                     RFGLGRFTTEEQIDFTIEQVTNTVNKLRDMSPLWEMYKEGIDLNTIEWAHH"
     assembly_gap    811..837
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<838..>1184)
                     /locus_tag="PK977_11355"
     CDS             complement(<838..>1184)
                     /locus_tag="PK977_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF18760.1"
                     /translation="NKDGIDLMAFTAHKMYGPKGVGALYVRRKNPRVKVTAQMDGGGH
                     ERGMRSGTLNVPGIVGFGKACELAMNEMKQDAELLSKLRDKLENALLSIEEAYLNGDK
                     EHRLPHVSNISFK"
     assembly_gap    1185..1263
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1264..1761)
                     /locus_tag="PK977_11360"
     CDS             complement(<1264..1761)
                     /locus_tag="PK977_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF18761.1"
                     /translation="MLQLPVYLDHNATTPCDPRVVEAMIPYFTQNFGNAASRNHPFGW
                     QAEEAVDYAREQVAKLVGADPKEIIFTSGATEGDNLAIKGVFEMYASKGNHIITCNIE
                     HKAVLDTCKHIEKEGGEVTYLKVKPNGLIDLAELEAAIKPTTILIAIMYANNEIGTVN
                     PVKEIS"
     gene            complement(1894..>4678)
                     /locus_tag="PK977_11365"
     CDS             complement(1894..>4678)
                     /locus_tag="PK977_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRF18762.1"
                     /translation="KTIAAVQQLIKFATENKTTLIPRAAGTSLAGQVVGNGIVVDISR
                     YFTHILEVNKEERWVRVQPGVIRDDLNHHLKQFGLMFGPETSTANRAMIGGMLGNNSC
                     GLHSIVWGSARDHVLEVKALLSDGSEVTFGKDDIRANGKDSFKSNIYHQLDALLSDEK
                     NQQLIRNKFPQEKVHRRNTGYALDYLLKMQPYNPQGESFNLCKLLAGSEGTLAFTTEI
                     RLNLIDLPPKEIAVVCIHCNSIVEAMRANIVALKLKPMAGELVDKYIMDFTIGHPEYS
                     KNRFFIEGDPAAILMVEFMEYSHDAAKEKAEQLVSELKQQQLGFAWPILYNEQTKYAW
                     DVRKAGLGLLRNIKGDAQPVNLIEDCAVTTDDLPAYVTDLQKVLEKHEVKASYYAHAA
                     AGELHVEPIINLKSPEGLKKFRSILADTVQLVKKYNGSLSGEHGDGRLRGEYIAEVVG
                     EETYRLFEEVKNIFDPNHVFNAGKITATPAMDTHLRVVQKPVQRKLQTTFSFSEQDGI
                     LRLAEKCSGSGDCRKMPVSGGVMCPSYMATRLEKDTTRARANILRQFLSNELDDQPFN
                     HKEIKEIMDLCLSCKGCKTECPSAVDVAKMKAEFLQQYYDRNGVPFRAKLIANFSKQM
                     KLVSKFPRAFNWFYGVPFFGRMAHTMVGFHPERTMPKMAKQSLQAWYKKRQKPKIQNP
                     GYKSVFLFCDEFTNYNDAEIGKKAILLLEALGYEVLIPQHVESGRTYLSKGLVKKAKE
                     IANRNIELLSGVISSETPLIGIEPSGILTFRDEYIDLANDTNYANAKALAAVAFTIEE
                     FLAKEYKAGRIERTLFTTEAKNILIHGHCYQKALSSQQYISTFLEIPVNYKTSLIPSG
                     CCGMAGGFGYEKEHYTISQQVGELVLFPAVRAAGKSDIIAASGTSCRHQVKDGTGKMA
                     LHPVEILFDALLP"
     assembly_gap    4679..4707
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4956..5360
                     /gene="mce"
                     /locus_tag="PK977_11370"
     CDS             4956..5360
                     /gene="mce"
                     /locus_tag="PK977_11370"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRF18763.1"
                     /translation="MQKIEHIGIAVKSLEISIPVFEKILNTPCYKTERVESEKVSTAF
                     FQNGPNKIELLESTDPDGVIAKYIEKKGEGIHHIAFAVEDIEAEMERLKKEGFVLLNE
                     KPKQGADNKLVCFLHPKHTNGVLIEICMERKD"
     gene            complement(5361..5903)
                     /locus_tag="PK977_11375"
     CDS             complement(5361..5903)
                     /locus_tag="PK977_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18764.1"
                     /translation="MKKSLPFLVFILVLIACNKPVKGPNGVTYKTAVAYNDYIVGRQS
                     TLIKNVLKFGEAAQVNLDSAEALLNKYVPETEKMITELKGMPAWKGDTALRNAAVKSF
                     TFYKRVFDQDYRRILEIRKNENATDEDIVEMNDIVDKISKEEEKFDKTFRNAQKDFAQ
                     KNKMKLIENDLQKKIDEARQ"
     assembly_gap    6021..6117
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6118..6692)
                     /locus_tag="PK977_11380"
     CDS             complement(<6118..6692)
                     /locus_tag="PK977_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRF18765.1"
                     /translation="MDFFKNLFTKHSGSTFKKELKNILGFKPGNLSLYKTALTHRSVK
                     ESIDENNERLEYLGDAVLSALIADYLFKRYPYKEEGFLTEMRSKMVNRQQLNDIAVRM
                     GLKKVTLYNKHDGSLKVSQIFGNTLEALVGAIYLDHGYKKASKWVFEQIIQPHMFMDD
                     LEILEINHKNKLYGWANKNGKVLEFETLNEK"
     gene            complement(6788..>7716)
                     /locus_tag="PK977_11385"
     CDS             complement(6788..>7716)
                     /locus_tag="PK977_11385"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRF18766.1"
                     /translation="IGGLITFQHEVTDFARGDGTPRFNPFFIPKMILDIAAGQISMRH
                     NLRGPNFAVVSACASSTNAIMDAFNLIRLGKSDIILTGGSEAVISEAGVGGFNAMKAM
                     SERNDDPKTASRPYDKDRDGFVMGEACGILVLEELEHAKARGAKIYCEIAGCGATADA
                     HHITAPHPEGLGAKNVMLAALDDAGMPDEVDYINTHGTSTPIGDGAEVKAITQVFGEH
                     AYKLNISATKSMTGHCLGAAGVIEAIASIQAVIHDMVPPTINHFTDDPELDPNLNFTF
                     NKAQQRTVRAALSNTFGFGGHNACVIVKKFGS"
BASE COUNT         2045 a         1716 c         1412 g         2311 t
ORIGIN      
        1 caacatggta aaattattta atttgtataa gaaaaatagt gaatacatac catttcatac
       61 tgaaactaaa atggtatgca ttctcttata attaggcctc agcgggaatg gtattcttgg
      121 tcttgtagtc ggcaatagcg ctttttattg catcttctgc caagacagaa cagtgaattt
      181 tgaccggcgg taaagctaat tcttctacga tgtccatatt gtctatggcc atggcttcat
      241 ctactgtctt cccttttagc cattcagtag ccaatgaaga agatgctata gcagaaccac
      301 agccaaatgt tttgaatttg gcatctttaa tgacgccggt agcttcatct acctcgatct
      361 gcaggcgcat gacatcaccg cattcgggtg cacccaccag gccggtaccc acattgtttt
      421 tacttttatc cagagttccc acattcttgg ggttctgata atgatcgatt actttttcac
      481 tatatgccat gactattagt ttttgtttta attaattgat gtttaatgat gtgcccactc
      541 aatggtattc aaatctattc cttccttata catttcccaa agcggactca tatctctaag
      601 tttattcact gtatttgtta cctgctcaat ggtaaagtca atctgctcct ctgttgtgaa
      661 acggccaagg ccgaatcgaa gcgaactgtg tgccaggtca tcacccaatc ctaatgcctt
      721 cagtacataa gatggctcga gagaagcaga agtacaggcc gacccagatg acaaagcaat
      781 atttttattg aagcccatca acaaaccttc nnnnnnnnnn nnnnnnnnnn nnnnnnnatt
      841 tgaaagaaat attggataca tggggcaaac ggtgttcctt atccccgttc aggtaggctt
      901 cttcaatact taataaagca ttttctaatt tatccctgag tttgctaagg agttcagcat
      961 cctgcttcat ttcgttcatt gccaactcac aggcttttcc aaagcctaca ataccgggaa
     1021 cattcaaagt accgcttctc atgcctcttt catggccacc accatccatc tgagcagtta
     1081 ctttaaccct tggattctta cggcgaacat acaaagcacc aacacctttt ggaccataca
     1141 ttttatgagc tgtaaaagcc atcaggtcaa taccatcctt atttnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnngctgatc tctttcacgg ggttcacagt accaatttca ttattggcat acatgatggc
     1321 tattagaata gtggtaggtt taattgccgc ttccagttct gccaggtcaa tcaaaccatt
     1381 aggttttact ttcaggtagg ttacttctcc gccttctttt tcaatatgct tgcaggtatc
     1441 gagcacagct ttgtgctcga tattgcaggt gatgatatga ttgcctttgc tggcatacat
     1501 ttcaaataca cctttaatcg ccaggttgtc accttctgta gccccggaag tgaaaatgat
     1561 ctccttagga tcagcaccaa ctaatttggc cacctgctcc ctggcataat caacagcttc
     1621 ttctgcctgc cagccaaagg ggtgattgcg gctggccgca tttccaaaat tttgggtgaa
     1681 ataaggaatc atagcttcca caacccttgg gtcacaggga gttgtggcat tatggtctaa
     1741 ataaacgggt aattgcaaca taaaataaag ggtaaaatat tagtttctac aacaacacaa
     1801 aagtacctca aaaggttcta aaagaaagga ttaaagctgc acgggagctt gaattttcgg
     1861 atttcgaaaa ggccctaact gccaccatga aaatcagggt aaaagagcat caaaaagtat
     1921 ctcaacagga tgtagcgcca tctttcctgt tccatccttt acctggtgac gacaggatgt
     1981 gccggatgca gcaataatgt ctgatttgcc ggctgcccgt actgctggaa acaaaactaa
     2041 ttctcccact tgctgagaaa tggtataatg ctctttctca tatccaaatc ctccggccat
     2101 accacaacaa ccggaaggaa tgagactcgt cttataatta acaggtattt ccaggaatgt
     2161 gctgatatac tgttgagatg aaagtgcttt ctggtaacaa tgtccgtgta tgagaatgtt
     2221 tttggcttcc gttgtaaata acgtcctctc aatcctgcct gctttatact cttttgctaa
     2281 aaactcttcg atggtgaatg caactgcagc caatgctttg gcattagcat aattagtatc
     2341 gtttgcaagg tctatatact catctctgaa agtaagtata ccactaggtt caattccaat
     2401 caaaggagtt tcagatgaaa taacacctga aagcaattca atattcctgt tggcaatctc
     2461 ttttgctttt tttactaacc ccttggacaa gtatgttctt ccgctttcaa catgttgtgg
     2521 aatcagcact tcatagccaa gcgcctctaa cagcaggatg gcttttttcc cgatttctgc
     2581 atcattatag ttggtgaatt catcgcaaaa cagaaagaca gatttgtaac ctggattttg
     2641 aatttttggt ttttgccgtt ttttgtacca agcttgcaag gattgtttcg ccatttttgg
     2701 catcgtccgt tctggatgaa atcctaccat agtatgtgcc attctcccaa aaaaaggaac
     2761 accgtaaaac cagttgaatg ccctgggaaa ttttgaaaca agcttcattt gcttactaaa
     2821 atttgcaata agctttgccc ggaatgggac tccgttcctg tcatagtact gctgcaaaaa
     2881 ttctgctttc attttggcta catccacagc tgaagggcat tcagttttgc atcctttgca
     2941 actcaaacag agatccatga tctcttttat ttctttatga ttgaaaggtt gatcatccag
     3001 ttcattgctt aaaaactggc gaagaatatt agccctggcc ctggtggtgt ctttttctaa
     3061 acgggtggcc atataactcg gacacatcac gccacctgaa acaggcatct tgcggcaatc
     3121 accggagcct gaacattttt cggcaagccg cagaatacca tcctgctctg aaaaactgaa
     3181 ggtagtttgc aattttcgct gaaccggttt ttgcaccact cttaaatgag tatccatagc
     3241 cggggtggcc gtaatttttc ctgcattaaa aacatggttg ggatcgaaga tattcttcac
     3301 ctcctcaaat aacctgtacg tttcttctcc cactacttct gcaatatact ctccccgcaa
     3361 tctgccatcg ccatgctcac ctgataagga accgttgtat tttttaacca attgtactgt
     3421 atcggccagt atgctcctga atttttttaa cccttcaggc gattttaaat taatgattgg
     3481 ctcaacatgc agttcgccgg ctgctgcatg ggcatagtaa gaggccttta cctcatgctt
     3541 ttctaatact ttctgcaggt ctgtgacata agctggtaag tcatcagtgg taacggcaca
     3601 atcttcaatc aggttcaccg gttgagcatc gcctttaata ttgcgcaaca accccaatcc
     3661 cgcctttcgt acatcccagg catattttgt ttgttcattg tataaaattg gccatgcaaa
     3721 tcccaattgc tgctgtttca gttcacttac taattgctct gctttttcct ttgctgcatc
     3781 atgactgtac tccataaact ccaccattaa gatggcagcc gggtcgccct caataaagaa
     3841 ccggttttta ctatactcag ggtgaccgat ggtgaagtcc ataatgtatt tatccaccaa
     3901 ctcaccggcc ataggtttaa gtttcaatgc cacaatatta gcccgcatcg cttctacaat
     3961 gctgttgcag tggatacaga caacagcaat ttcttttggg ggtaaatcaa tcaggttgag
     4021 tctaatttcc gttgtaaacg ccaatgtacc ctctgaccct gccaatagtt tacacaggtt
     4081 aaaagattcc ccttgcggat tataaggctg cattttgagt aaatagtcca gcgcataccc
     4141 tgtattacgc cgatgtactt tttcctgcgg gaatttattt cggattaatt gctggttttt
     4201 ttcatcggac aacaatgcat caagctgatg gtaaatgtta ctcttaaatg aatcctttcc
     4261 gtttgccctg atatcatctt ttccgaaagt cacctcagac ccatcactta ataatgcctt
     4321 tacctccagt acatgatctc ttgcagaacc ccagacaata ctatgcaatc cacaagaatt
     4381 attacctaac ataccaccga tcatggccct gttggcggtg gaggtttcag gaccaaacat
     4441 caacccaaac tgtttcaggt gatggttcag gtcgtcacgg atgacgccgg gctgtaccct
     4501 tacccacctc tcttctttat tgacctcaag aatatgtgtg aaatacctgg aaatatcgac
     4561 cacaatgcca ttgccaacta cctgtccggc caatgaagta ccggctgccc tggggattag
     4621 cgttgtttta ttttcagtag caaattttat tagttgttga acagccgcaa ttgtttttnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnttt cctgatacac cgatgcgtct gtagaataga
     4741 tcctcaactg tgcctggtga atgggtgaat catcataaaa aagaagtcca tcaaaatttc
     4801 tttccagttg ttttaattgc tgcagcattt tgtattctga ttggtcgttt acaaatctac
     4861 caacttccgt catgtgaaaa actgataaaa gtttatttac cggtcataaa atagaaatag
     4921 caaagcgggt aactttgaag agcaaatcat caatcatgca aaaaatagaa catatcggca
     4981 tcgcagtaaa aagccttgaa atatccatcc cggtcttcga gaaaatactg aatacaccct
     5041 gttacaaaac cgaaagggtg gaaagtgaaa aagtaagcac tgcctttttc caaaacggtc
     5101 ccaataaaat tgaattactg gaaagcactg accctgatgg tgtgattgcc aagtacattg
     5161 aaaaaaaagg agagggtata caccatattg cttttgcggt tgaagatata gaagccgaaa
     5221 tggaacgact aaaaaaagag ggatttgttt tattaaatga aaaacccaaa caaggcgccg
     5281 ataataaact agtttgcttt cttcacccca aacacactaa cggagtcctt atcgaaattt
     5341 gcatggaacg aaaagattga tcattgcctg gcctcatcga tcttcttctg caggtcattc
     5401 tcgatcagct tcattttatt cttttgagca aaatctttct gtgcatttcg aaaagtcttg
     5461 tcaaattttt cttcttcctt gctgatctta tcaacaatat cattcatttc aacgatatct
     5521 tcatcagttg cattttcatt tttacggatc tcaagaatgc ggcggtagtc ctggtcaaaa
     5581 acccttttgt aaaaagtaaa tgattttacg gctgcattgc gtaaagccgt gtcccctttc
     5641 caggcaggca tacccttcaa ctctgtgatc attttttcag tttccggaac atacttattc
     5701 agcagggctt ccgcagaatc caggtttacc tgtgcagctt ctccgaactt caatacgttt
     5761 ttaataaggg tactttgtct gccaacgata taatcgttat aagcaacagc agtcttgtaa
     5821 gtaactccgt tcggaccttt aaccggctta ttacaggcaa ttagcaccag tatgaaaact
     5881 aaaaaaggca ggcttttttt catctgcttt atttgaatgg ttcttttcaa tcaatattct
     5941 tcccccactg aatcagcatt aatgataccc agtttttcta cggctacctg tgcagcaatt
     6001 tgtgaagcat cttttttatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
     6121 ttttcgttca gcgtttcaaa ttcaagcact tttccgttct tgttggccca tccgtatagt
     6181 ttgtttttat gattgatctc cagaatctcc aggtcatcca tgaacatatg tggttgaatg
     6241 atctgctcga atacccattt actggccttt ttgtagccat gatccaggta aatggccccc
     6301 accagtgctt ccagagtgtt accgaagatc tggctgacct ttaatgagcc atcatgctta
     6361 ttataaagtg tgaccttttt caaccccatc cttacagcaa tatcattcag ttgctgccgg
     6421 ttcaccattt tgctgcgcat ttccgtcaga aagccttctt ctttgtaggg atatcgttta
     6481 aaaagataat cggctatcag ggcactgaga acagcatcgc ctaaatactc gaggcgttca
     6541 ttgttctcat caatgctttc ttttacagag cgatgggtaa gggctgtttt atatagcgac
     6601 aaattcccgg gcttaaaacc aaggatattt ttaagctctt tcttaaaagt tgaaccggag
     6661 tgtttggtaa ataggttctt gaaaaaatcc acggccgtta agctacaaaa aagttgacag
     6721 ctaataaagt ttaagcattg aaaacttaca atgttagctt caggtctgtc cttttcccgg
     6781 ttaaaaatta gcttccaaac ttcttaacta taacacaggc attatgtccg ccaaatccaa
     6841 atgtattgct taatgccgcc ctaactgttc gctgttgtgc tttattgaaa gtaaaattga
     6901 gattgggatc cagttccgga tcatctgtga agtgattgat cgtgggcggt accatatcat
     6961 gtatcactgc ctgtatactg gcaatagctt ctataacgcc ggccgcacca agacaatgtc
     7021 ctgtcataga tttggtagcg ctgatattca gtttataagc atgttcacca aagacctggg
     7081 ttatcgcttt cacttcagct ccgtcaccta tcggtgttga tgttccgtga gtgttgatat
     7141 aatcaacttc atcaggcata ccggcatcgt ccagtgccgc cagcattaca ttctttgcgc
     7201 ccagcccttc gggatgtggt gcggtgatat gatgtgcatc agcagtggct ccgcagcctg
     7261 caatttcaca ataaatttta gcacctcttg cttttgcatg ttcaagttcc tccagtacga
     7321 gaataccaca tgcttcgccc atcacaaagc catccctgtc tttatcataa ggacggctgg
     7381 ctgttttggg atcatcattc cgctcactca tggccttcat ggcattgaag ccacccacac
     7441 cagcttcgct gattacagct tcgcttcctc cggttaaaat aatatcagat tttcccagcc
     7501 tgatcaggtt gaaagcatcc atgattgcat tggtactgct ggcgcaggca cttaccacgg
     7561 caaaattggg tccgcggaga ttatgacgca tactgatctg tccagcagct atatctaata
     7621 tcattttggg aatgaaaaag ggattgaaac gcggagtacc atcacctctt gcaaaatccg
     7681 tcacttcatg ctgaaatgtg ataagaccac cgatac
//
LOCUS       DAORLS010000143         7674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5170_length_7674_cov_2.134270, whole genome shotgun sequence.
ACCESSION   DAORLS010000143 DAORLS010000000
VERSION     DAORLS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_7674_cov_2.134270"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..3213
                     /locus_tag="PK977_11390"
     CDS             220..3213
                     /locus_tag="PK977_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF18767.1"
                     /translation="MIKKIAALLFICLPFLICVNAAAQTQQNPLLFNQLTTRDGLLSN
                     ITTTLYQDSRYFLWIGTNHGLIRYDGKFFTNYNQIGEGGITDRVITCITEDADGNIWI
                     GTESGLNKLNPKNNQITQYRSGSGPGTIPFHWCNYLFTDSKKQLWLTTEKGIALYDKK
                     TDSFENIPISVYGKDEKINKFINQVAEDEEGNFWLSTSFGLKKFNPSTKQFYSYHLEE
                     PNNSLRKENVLYGLCVAKPGSIWAVSFEGHLLHYESKNNRLKIVSISSKEGPRLLSIC
                     TIKQEKQKFLLIATSDGIFKVNPDNPESPPQKLLSGKSLNKVFTDKQNNLWISSENGL
                     FRQIHVNIALQWHDVPDKDKPLINHIIRSTLKENIYFLTSRSGWFTYNSNNDTVTFHT
                     LPADDRGLLTQINRHVGDENGYWFTSTNGFGYYDLKKNRLIDLTRLITKVSSQQSTGF
                     IEEPLKGQLWITMRRSGILVYNTLTGKDTVLFADKQKADNIYGKTVFDFKKGPDNNIW
                     FTTDEKLYVVNPVTYSYKTYKADNNIFQNNKTNPYRILFTKDGRALINSQSNIYEFKN
                     QTLRVIHPKKGLFNFELQRLLEDETGNIWAVTDNGIYKTNNQFSYWKNISNAQGFENE
                     TAGYEINLSVPGKVMITSVGKIGVLDNKLMEQSRVPNPSIISRIRLGNTEAHFPVPAL
                     KYKCDYKDAVEIELTSPDFSNESENSIYYRLEGWDDEWRRLSTTNTIRYEQLPPGNYL
                     FSTKSVNTESIESRIAFISFKVVPPFYRSWWFIVLSFIIIISIAYLFYRYHLHKAIEL
                     EKIRTRIATDLHDDIGATLSSISMYSDALKLQVKDNMPQLESVLTKMGESSREMVTGM
                     SDIVWAINPDNDSGEKLLNRMENYVTDMCAVKNIKLFFKADEKLKQIRLPLEKRKNIY
                     LVFKEAVNNAVKYSMADHLWVTVNIGAEFSLLIKDDGAGFTMTDVKKGNGLKNFKTRA
                     DEIKGKIQINSMPGAGTSVKLICQL"
     gene            3266..3892
                     /locus_tag="PK977_11395"
     CDS             3266..3892
                     /locus_tag="PK977_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18768.1"
                     /translation="MKIVIFEDNKKFRESLEFLIVTSTDMELVGSFEDTNRLLSRIEA
                     LQPDVVLMDINIPGKNGIEAVKEIKDKFPDVQVCMQTVFEDQDKIFASLCAGASGYIL
                     KNTSSEKILQAIRDVAEGGSFFTPSVAKKVLQSFQQQPEKAEYIQLTDKEKEVLKYLV
                     EGLSYKMIAEKVFLSFHTVHTHLRHIYEKLHVNSKSEAVAKAIKNKLV"
     gene            3959..>4255
                     /locus_tag="PK977_11400"
     CDS             3959..>4255
                     /locus_tag="PK977_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18769.1"
                     /translation="MKKLVLVLLLTILFLPVTFAQNVGIGTTTPVTRLHVSGTPSNIA
                     TFNGGGQMWITLSESSIDRGYIGSFTGNPEDVDFGTYLSNTTGKVHLTTSNQPRL"
     assembly_gap    4256..4406
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4407..5195
                     /locus_tag="PK977_11405"
     CDS             <4407..5195
                     /locus_tag="PK977_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18770.1"
                     /translation="NNGPLSLSSNAYWNGSAWVYPNTGNNSLYLLLHQPNQAFEFRVR
                     PDGGSQSTAMVVKVNGNVGIGTNNPAAKLDINGDVNIQNRVLLNNAAGNAGQALISNG
                     NASSPTWQNIAYSNNDRFMFISSNLNSKSGAFLDTILFNTAYALSGAVSYNSNGLFTI
                     NKSGLYSIKGNFSSNISCTAAGLAYSAVLFYPSGSITYLGNQSKQLVANGTNFIDAET
                     ISIDMQIYFSAGSTFFFYALLSSTSAITNSSFYQSPISINLISE"
     assembly_gap    5553..5562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5805..>6404
                     /locus_tag="PK977_11410"
     CDS             5805..>6404
                     /locus_tag="PK977_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18771.1"
                     /translation="MKTSSAFLWQLIRSMTANEKLFFKRNYALNGYGSKPLYLKLFDA
                     IAAQKKYNEEAILKKFSPELTKKNITFQKHYLQQQVSDAIAQYDNRNSAGHDIYKQIL
                     LIRVYRKKGLLDEAHALWKKAVVKARTTESYAKLNLLKTEFEKMILFSSVHTSYDDLH
                     SVFKGNIITYTEYAEMITLRDIYTEVLLLKRKAHFDLDDE"
     assembly_gap    6405..6428
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6429..7356
                     /locus_tag="PK977_11415"
     CDS             <6429..7356
                     /locus_tag="PK977_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18772.1"
                     /translation="LLERVNATNTTPNRRSFWFRHYFGMSKATLLYLLQDISTAFALL
                     KDAWQDWRENQNFIGTDGEFYIEVLYMINYAGILRGDYSYVQNTFNDQVNELITDPVQ
                     RANFEVIKFLAFNKIYNKTAKYDEVEKLNRFMKSRYQQWEPVLNADLNRTTNLSLGIG
                     SFVLEQYDEALYYIKRGITYFKEGVREEHEAVAQILLLLTSYCMDNPKLFDAQYRTTY
                     NYFYKRKKKQPFETALVQCLHRTFYMKEMKGRVEEYRKTLAVFEANQDNVVQQLNLAI
                     FNYFGWLESRAQRIGYRQYVEQKVKKESARQS"
     gene            complement(7419..>7674)
                     /locus_tag="PK977_11420"
     CDS             complement(7419..>7674)
                     /locus_tag="PK977_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18773.1"
                     /translation="ISLCQASVEPAQKKMTEMNMPFRRPYRYLFVNEKKKKAITANKH
                     TDNMISRADRGGLLCHWKIMPAASASSNLKKGVFFQKSAP"
BASE COUNT         2495 a         1418 c         1451 g         2125 t
ORIGIN      
        1 gccataaaag agccggtatt cagctttaat aaatttcccg gggtaaataa agaactggga
       61 cctgaaatga aatcaaccgg agaagctatt cgcttcatca aaaacctgag ggacccgtat
      121 ttcaggcaac tatataaaga caggagtatg tacctgagta aatagacatc actactttgt
      181 ggtatatcta tcatttcgtt ttgccgctat ctttataata tgataaaaaa gatagcggct
      241 ttgcttttta tctgtcttcc atttctaatc tgtgtaaatg cagcagcgca aacacagcaa
      301 aatccgcttt tgttcaacca acttaccacc agggatggat tgctgagcaa cattaccacc
      361 accctttacc aggatagccg ttacttctta tggattggca ctaatcatgg tttaataaga
      421 tacgacggaa aattttttac aaactataac cagatcggag aaggtggcat taccgaccgg
      481 gttataacct gtattactga agatgctgac gggaacatct ggatcggcac tgaaagtggg
      541 ctaaataaac tcaatcctaa aaacaaccag attacccaat acaggtcggg aagcggcccg
      601 ggtaccattc ctttccactg gtgtaactat ctttttaccg actcaaaaaa acagctttgg
      661 cttactactg aaaaaggtat tgccctctat gacaaaaaaa ctgacagttt tgagaatatt
      721 ccgatatctg tttacggaaa agatgaaaag ataaataagt ttattaacca ggtagctgaa
      781 gatgaagagg ggaacttctg gttaagtact tcatttggtc ttaaaaagtt caatccctcc
      841 acaaaacaat tctattctta tcatttagaa gagcctaata attctttacg gaaagaaaat
      901 gttttgtacg gattatgcgt ggcaaagccg ggaagcatct gggctgtatc attcgaaggt
      961 catttgttgc attatgaaag taaaaacaac aggttgaaga ttgtctccat cagcagtaaa
     1021 gaaggccccc ggctactctc aatctgcaca atcaaacaag aaaaacaaaa atttctgctt
     1081 atagcaacat ctgatgggat ttttaaagta aatccggata atccagaatc accacctcaa
     1141 aaattgctga gcggaaaatc actcaacaaa gtgtttactg acaagcaaaa caatctctgg
     1201 atatcttcag aaaatggcct attcaggcaa atacatgtga atattgcact gcaatggcat
     1261 gatgttcccg ataaggataa accacttatt aaccatatca tacgatcaac gcttaaagag
     1321 aatatttatt ttttgaccag caggtcaggc tggtttactt ataatagtaa taatgatact
     1381 gtaaccttcc atactttgcc tgcagatgac agggggttgc tcacacaaat aaaccgccat
     1441 gtgggagatg aaaacggata ttggttcact tccacaaatg gctttggcta ctatgatctt
     1501 aaaaaaaacc ggctgattga tttaacaagg ctgattacaa aagtatcaag ccagcaatcc
     1561 actggcttca ttgaagagcc tttaaagggc cagctttgga taaccatgcg gcgaagcggt
     1621 atccttgtct ataacacact taccgggaaa gacacagtat tgtttgccga caaacaaaaa
     1681 gcagataata tttacggaaa aacagttttt gattttaaaa aaggacctga taacaacata
     1741 tggtttacaa ccgacgaaaa actatatgtt gtaaacccgg taacatacag ctataaaaca
     1801 tataaagccg acaataatat ctttcaaaac aataagacaa atccttatcg gatacttttt
     1861 acaaaagatg gcagagcttt gataaatagc cagtcaaaca tttatgaatt caaaaatcaa
     1921 acacttcggg taattcatcc gaaaaagggg ctttttaatt ttgaactgca aaggctcctg
     1981 gaagatgaaa ccggtaatat ctgggccgtt accgataatg gtatttataa aactaacaac
     2041 caattttctt actggaaaaa catcagcaat gcacagggat tcgaaaatga aacagccggt
     2101 tatgaaatca atctttctgt cccgggcaag gtgatgatta cctctgttgg aaaaattggt
     2161 gtgttggata ataaactaat ggaacaaagc agggtgccga atccatccat tatcagccga
     2221 atccgcctgg gcaacacaga agctcatttc cctgttcctg cacttaaata caaatgcgat
     2281 tataaagacg ctgtagaaat agagttaacc tcacctgact ttagtaatga atcagaaaac
     2341 agtatttact acaggctgga aggctgggac gacgaatgga gaaggctaag cactaccaat
     2401 acaatcaggt acgaacagtt acccccgggt aattatttat tcagtacaaa atctgttaat
     2461 acagaaagca tagaaagcag gattgcattt atttctttta aagttgtgcc gccgttttat
     2521 cgaagctggt ggttcattgt cttgtctttt ataattataa tcagcattgc ctacctgttt
     2581 tacaggtacc atctgcataa agccattgaa ttggaaaaaa tacgaacgag gattgctact
     2641 gacctgcatg atgatatcgg agccactctg agttctatat ccatgtacag cgatgccctc
     2701 aaattgcagg tgaaggataa tatgccacaa ctggaatctg tactcaccaa aatgggagaa
     2761 agcagccggg aaatggtaac tgggatgagt gatattgtat gggcaataaa tccggacaat
     2821 gacagcggtg aaaaattact taacaggatg gaaaattatg taactgacat gtgtgcagta
     2881 aaaaatataa aactattttt taaagctgat gaaaaactaa aacaaatcag attgccatta
     2941 gaaaaaagaa aaaacattta cctggtattt aaagaagcgg taaataatgc ggtaaagtat
     3001 tctatggccg atcatttatg ggtgactgtg aatatagggg cagagtttag ccttttaata
     3061 aaggatgatg gagcagggtt tactatgaca gatgtaaaaa aaggtaacgg tttaaaaaat
     3121 ttcaagacca gggctgatga aataaaagga aaaattcaga ttaactcaat gcccggtgct
     3181 ggcacatccg ttaaattaat ctgccagctc tgataccaca aattggcgat gggtacatgt
     3241 tttttttatg atcaaatttg ctaaaatgaa aattgtaatt tttgaagaca ataagaaatt
     3301 cagggaaagc cttgaatttt taattgtaac gagtacggat atggaactgg tgggttcgtt
     3361 tgaagataca aaccggctct tatcaagaat tgaagcattg caacccgatg tggtattgat
     3421 ggatatcaat atccctggta aaaatggaat tgaagcagta aaagaaatca aagacaaatt
     3481 tccggatgtt caggtgtgta tgcaaaccgt atttgaagac caggataaga tatttgcctc
     3541 cctttgtgcc ggtgcctcgg gttatatatt aaaaaacaca tcgtctgaaa aaattttaca
     3601 ggctatccgc gatgttgccg aaggcggaag tttcttcaca ccatccgttg ccaaaaaagt
     3661 tttacaaagc tttcagcagc aacctgaaaa agcagaatac atacagctga ctgataagga
     3721 aaaagaagta ttaaaatacc tggttgaagg gcttagttac aaaatgatag ccgaaaaagt
     3781 tttcctcagt tttcataccg ttcacactca tcttcgccat atttatgaaa agctacatgt
     3841 aaacagcaaa agcgaagcag tggccaaagc cataaagaat aaactggtgt aatcatcacc
     3901 aattttggcg atatagggat gtttatatct ttttcatatt tgaaacaaaa tacaggatat
     3961 gaaaaagctg gttttggttt tacttcttac aattttattt ctccccgtaa ctttcgcaca
     4021 aaatgttggt atcggtacta ctacacctgt tacccggtta cacgtttccg gtacgccaag
     4081 caatattgct acttttaacg gcggtggcca aatgtggatc acattatccg agagtagtat
     4141 tgacaggggt tatatcggtt cttttacggg taatccggaa gatgtggatt ttggcaccta
     4201 tttatcaaat actacaggaa aagttcacct taccacttct aaccagcccc gcctcnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnaata atggtcccct cagtctcagc agtaatgctt
     4441 attggaatgg ctcggcatgg gtatatccca ataccggtaa taactcttta tacctgctat
     4501 tgcaccagcc caaccaggct tttgaattca gggtaaggcc cgatggcggc tcacaaagta
     4561 cagccatggt agtgaaagta aatggtaatg tggggatagg aaccaataat cccgctgcaa
     4621 aattggatat taacggagat gtaaatatcc agaaccgggt attattaaac aatgctgcag
     4681 gcaatgcagg tcaagccttg ataagtaatg gaaatgccag cagcccaacc tggcaaaata
     4741 ttgcctatag caataatgac agatttatgt ttatttcgtc aaaccttaac agcaaaagcg
     4801 gtgccttttt ggataccatt ttatttaaca ccgcctatgc attgtccggt gctgtcagtt
     4861 ataattccaa cggattgttc acaataaata aaagcggatt atactctatt aaaggaaatt
     4921 tttcaagtaa tataagctgt acagccgctg gacttgcata ttcggctgtt ttattttatc
     4981 cttcaggtag tattacatac cttggcaacc aaagcaagca actagttgca aatggaacaa
     5041 attttataga tgccgaaacg atttccattg atatgcaaat ctatttttca gcgggatcta
     5101 cattcttctt ttacgcattg ctgtcttcca cctccgcaat taccaattct agtttttatc
     5161 aaagtccgat aagtattaat ttgatttccg aataagttac aggttatgta aaagcaatcc
     5221 ctagttttac ttcttaccat tttgtttctt cgaaaaatca gtaccaatca gttttaatta
     5281 ttacttacca gctggttccc agtttgtttt agaatattct gttgcatcag ccggagctat
     5341 ttcttcaata attgtacatt cattacctct tgaaatacac ttaataaatg aataggactt
     5401 cgtaatttaa aatacgaatc aatacattta actatcagcg atactttcgg ttgtatttac
     5461 agctcaaaca ttgacaagca tgtattaata ataaagccca tcttttcaga tgggctttag
     5521 tttaatggtt aatggttcag actagtcccc gcnnnnnnnn nnggttttgg ttagcataaa
     5581 tatttttttg ctcagggata tattcttgta tgaaaataga cgactactcg ttgagtgcaa
     5641 ttccttattt atcctattat ttgtatcact tcgtatataa ttatcaccaa tattacactt
     5701 aaatatatat taatcagcca cttaactata attaaatact ggtttagatt tgacacatat
     5761 agccgaattt atgtacttta gtttgtaagt aaatcaacct ttttgtgaaa acatcttccg
     5821 catttctgtg gcaattgatc cgttcaatga cggcaaatga aaaattgttt ttcaaaagaa
     5881 actacgcatt gaacgggtac ggatcaaaac ctttatacct gaaactgttt gatgcaattg
     5941 ctgcacaaaa aaaatacaac gaagaagcta tcctgaaaaa attcagtcct gaactgacaa
     6001 agaaaaatat tacctttcag aaacattacc tgcagcaaca ggtaagtgat gccattgcac
     6061 aatatgataa ccgcaacagt gccggccatg atatttacaa acaaatactg ctgataaggg
     6121 tgtaccgaaa aaaaggacta cttgacgaag cacatgccct ttggaaaaaa gcagtagtaa
     6181 aagcaaggac tacggaatca tatgcaaagc tcaacctgct gaagactgaa tttgaaaaaa
     6241 tgatcttgtt cagcagcgta cacaccagtt acgatgacct gcactccgtg ttcaaaggca
     6301 atattatcac ctacactgag tatgcagaaa tgatcaccct gcgggatata tacactgaag
     6361 ttttactgct gaaacgtaaa gcacattttg acctggatga tgagnnnnnn nnnnnnnnnn
     6421 nnnnnnnngc tgctggaaag agttaatgca accaacacaa ctccaaacag aagatcattt
     6481 tggtttcgtc attattttgg catgagcaaa gccaccctgt tatacctgct tcaggatatt
     6541 tctacagcat ttgctttatt aaaagatgca tggcaggact ggagggaaaa ccaaaatttc
     6601 attggaacag atggagaatt ttatattgaa gtattgtaca tgatcaatta tgcagggatt
     6661 ttgagaggcg attattccta tgttcaaaat acatttaatg accaggtaaa cgaattgatt
     6721 accgaccctg tacagagggc caattttgaa gtgataaaat tcctggcttt taataagata
     6781 tataataaaa cagcgaagta tgatgaagtg gagaagctaa accgctttat gaaaagccgc
     6841 taccagcaat gggaaccggt actgaatgcc gatctaaacc gtacaactaa tctgtcgctg
     6901 ggaattggct cctttgtgct ggaacaatat gacgaagcgc tgtattatat aaagagggga
     6961 attacctact ttaaagaagg agtaagggag gaacatgaag cagtggccca aatactgctg
     7021 ctgctaacat cttattgcat ggacaaccca aagctgtttg atgctcaata ccggaccacc
     7081 tataattatt tttataaacg aaaaaagaag cagccctttg aaacagcact ggttcagtgc
     7141 ctgcaccgca ctttttatat gaaagagatg aaagggcggg tggaagagta ccgtaaaaca
     7201 cttgctgtat ttgaagccaa ccaggataat gtagttcagc aactgaacct ggctattttt
     7261 aattattttg gctggttaga aagccgggcc caacggatag gttaccggca atatgtggag
     7321 caaaaagtaa aaaaggaatc agcacggcaa tcctgacgaa tacccggaaa gtagtattgc
     7381 cgggtatttc gggagttatt acttttcagt atgcaggtct atggcgctga tttctggaag
     7441 aaaacccctt tcttaaggtt actgctggca ctggcagcag gcattatctt ccaatggcat
     7501 aataaaccac ctctatctgc cctgctgatc atgttatctg tatgcttatt ggctgtaatg
     7561 gcctttttct tcttctcatt aacaaaaaga taccggtatg gcctcctgaa cggcatattc
     7621 atttccgtca tcttcttttg tgccggttcc acacttgcct ggcataaaga tatc
//
LOCUS       DAORLS010000144         7651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5185_length_7651_cov_2.289758, whole genome shotgun sequence.
ACCESSION   DAORLS010000144 DAORLS010000000
VERSION     DAORLS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5185_length_7651_cov_2.289758"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>653
                     /locus_tag="PK977_11425"
     CDS             37..>653
                     /locus_tag="PK977_11425"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF18774.1"
                     /translation="MAGWLIIMRPRYALISGVALLFSIKSILVSFAFNSPGSFEYKRT
                     PGTIRVVTWNVARFIELKKVNNKGSATRLKMLDLLKQQEADVLCLQEFHTSTKADYYD
                     NITAIKEELGYPYHYFNFDTDGDEMFYSSIIFSKHPIVDSGTVRYPRPTLPDVLIHAD
                     IKFNSDTFRVYTTRLQSLRFNKMDYKNLDKIKSGDYSLISNSKTI"
     assembly_gap    654..710
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <711..984
                     /locus_tag="PK977_11430"
     CDS             <711..984
                     /locus_tag="PK977_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HRF18775.1"
                     /translation="ITGISPHPVIICADLNETPNSYTYRKVKGEMQDAFLKKGFGIGR
                     TFSGLSPTLRIDYIFADKRFKVRQFNRVVKNLSDHYMLVADVELRK"
     gene            1095..>1826
                     /locus_tag="PK977_11435"
     CDS             1095..>1826
                     /locus_tag="PK977_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF18776.1"
                     /translation="MGDLKVYNSYSRQKEIFTSITPGHVGMYVCGPTVSGESHLGHAR
                     PYITFDVIYRYLQYLGYKVRYVRNITDAGHFEEEGREAVDKIATKAVLEKLEPMELVT
                     KYTNLFHWAMEQFNTLKPSIEPTATGHIVEQIEMIKQIMEAGYAYEVNGSVYFDVKKY
                     AAGHDYGKLSGRVIEDLLETTRELEGQEEKRDTADFALWKNAAPEHIMRWQSPWGVGY
                     PGWHIECSAMATKYLGAQFDIHGGGM"
     assembly_gap    1827..1857
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1858..>2472
                     /locus_tag="PK977_11440"
     CDS             <1858..>2472
                     /locus_tag="PK977_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRF18777.1"
                     /translation="EIAQSTICNHSAPVRYWIHNNMITINGRKMGKSYNNVIKLTELF
                     SGDHPILEKAYHPMTVRFFILQTHYRSTLDFSNEALQASEKGLKRLWDAYEKLGNLSI
                     DDSQQAIDAELDEKIRKQIAEFDEFMNDDFSTAKVLANMFEIVPVINSMKDKIIPVSA
                     LSKETFELLQTSFKTWLEDIFGLQSVSEADNAKLQGVMQLLIDIR"
     assembly_gap    2473..2519
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2792..>3333
                     /locus_tag="PK977_11445"
     CDS             2792..>3333
                     /locus_tag="PK977_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF18778.1"
                     /translation="MKLKNLISRTSLFLLLSAPVVLISCDKDDDTATSNTITDIVVAN
                     GDFSTLEAAVVRANLQTTLSGSGPFTVFAPDNAAFSAAGITTSVLNSLSPAQVQSILL
                     YHTLGSRVNAANVPAGPNAKVTTASGDSVFVTRDARGVFVNGIQVTTADISADNGVIH
                     RIGRVLNPPVGNIVETAVASG"
     assembly_gap    3334..3344
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3345..3741
                     /locus_tag="PK977_11450"
     CDS             <3345..3741
                     /locus_tag="PK977_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF18779.1"
                     /translation="LVKAVTRATTAPGGDPTLAGTLGSATLTVFAPTNAAFTQLLGAL
                     SLTDINQIPIGTLLAVLRYHVVGGRAFSSDLTNGNLAMLAGGNTTVSLTTGATIRGNG
                     NGGNASNITATNIVCRNGVVHLIDRVLLP"
     gene            3764..4855
                     /locus_tag="PK977_11455"
     CDS             3764..4855
                     /locus_tag="PK977_11455"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF18780.1"
                     /translation="MKNLVGPVSAVAGPIYQLGSKMFNHRYRYLFIGLLALYTFLNTE
                     LCNVYHYFDIEIEWYYAFLTILLITGFTWEGNRLLEPFIQQKVQPQRNKWRYLGIFFI
                     AGNIVASLAAIAVVYFIGNVLHGYSWQQNLNPLKLNIIYVSLVNLFFHLVNAIFFFFR
                     EYRTQWMEAEELRRSSTQAELEMVKSQINPHFLFNNLNVLSGMVIKDNPEANKFIEEF
                     SKVYRYILSNQQKELVELRSELDFIQPYIFLLKKRFDKGLVIDINVADRYKNLYVVPA
                     ALQMLIENAIKHNVVSQHKPLHIDIHSNGNETLVVKNNLQPRTVNEPSSRIGLQNIRR
                     RYEIVSGREVEIKKSTTDFEVTLPLLNLN"
     gene            4862..5614
                     /locus_tag="PK977_11460"
     CDS             4862..5614
                     /locus_tag="PK977_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF18781.1"
                     /translation="MKLIIVEDEKLAAERLKTLVKEYDPSIEVLACLESVEETVQYLR
                     HHAAPDLLLLDIHLSDGHSFEIFSQVYYKGPVIFTTAYDQYALEAFKMFSIDYILKPV
                     TLEALATAMNKFRNLSASFASADYRQLMPQLSSREYKKRFLGKVGQRLFFVDTLDIAY
                     FQADNKVVYLVDKEGNRYVVEHTMEKLMAMVNPSEFFRLNRRYIVRINAIQQVKPYFN
                     GRLKLSVKGSQQPEEMVISRERVSEFKQWAEA"
     assembly_gap    5694..5747
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5748..>6210)
                     /locus_tag="PK977_11465"
     CDS             complement(<5748..>6210)
                     /locus_tag="PK977_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HRF18782.1"
                     /translation="SGIFDYIGVHQVLEIGFGTGLNALLTLIEADRHKNRIYYTAIEP
                     FPLDEPLIAQLNYCEQLNLSQYQQLFEKMHQCGRGEMYEITQYFRLTKHNTTLQEFST
                     DEVYSIVYYDAFAPAAQPELWTKEIFENLYSMMAEGGLLVTYCSKGDVQRA"
     assembly_gap    6211..6359
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6401..7018
                     /locus_tag="PK977_11470"
     CDS             6401..7018
                     /locus_tag="PK977_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRF18783.1"
                     /translation="MEYVAHLSKDKKLKRLLESSELHQLKKRKNICTYLCASIMSQQL
                     STKVATVIHNRFLALYGGKEPLPQQILDTPFEKLRSIGLSNAKANYVKNVAQFEIDFG
                     MDAKRLHKMDNEEVIAYLTQIKGVGRWTTEMLLMFALGREDVFAVDDLGIQNAMISLY
                     KLDNSHKKKLREDMLRISQKWSPYRTYACLHLWQWKDNTPVKKKQ"
     gene            7076..7303
                     /locus_tag="PK977_11475"
     CDS             7076..7303
                     /locus_tag="PK977_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HRF18784.1"
                     /translation="MKQIKSFTVVFSVMLLIFSGSCQKSSDECIDESRMSNNPCTLEY
                     NPVCGCDGVTYSNPCFADRAGVTRTTPGVCP"
     gene            7334..>7651
                     /locus_tag="PK977_11480"
     CDS             7334..>7651
                     /locus_tag="PK977_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRF18785.1"
                     /translation="MHKWLPALLLPVMFSFCSGKQRSDEWISLFDGKTLHGWKVGANA
                     SAFNVENGSIVVNGNVSHLYYDGKVQQHQFKDFEFKAEVMTTPGSNSGIYFHTQYQEK
                     DWPK"
BASE COUNT         2133 a         1530 c         1650 g         1989 t
ORIGIN      
        1 cggaattgaa agccgatacg ctgccattct gctttcatgg ccggctggct cattatcatg
       61 cggccacggt atgcactgat ctcaggagtg gccttgctgt tcagcattaa aagcatcctg
      121 gtttcttttg cgtttaatag cccgggtagt tttgaataca aacgaactcc cggcaccatc
      181 agggtagtta cctggaatgt ggcccgtttt attgagttga aaaaagtgaa caataaaggc
      241 agcgctaccc ggctgaaaat gctggatctg cttaaacagc aagaagcgga tgtgctctgc
      301 ctccaggaat tccatactag tactaaagct gattattacg ataatataac tgctattaag
      361 gaagagctgg gttatcccta ccattacttc aattttgaca ccgatgggga tgaaatgttt
      421 tacagctcaa ttattttttc gaaacacccg atcgttgaca gcggtacggt gcggtaccca
      481 cggcccacat tgcccgatgt actgatacat gccgatataa aattcaatag cgatactttc
      541 cgggtctata ccacgaggct tcagtcctta cgtttcaata aaatggacta caaaaacctg
      601 gataagataa aaagcggcga ctacagcctg atcagcaatt caaaaaccat tttnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gattacgggt
      721 atcagtccgc atccggttat catctgcgct gacctgaatg aaactcctaa ttcatataca
      781 tacagaaaag tgaaaggcga gatgcaggat gcctttttga aaaaaggatt tggtatcggc
      841 agaacgtttt ccgggctttc acccaccctt cgtattgatt atatttttgc tgacaaacga
      901 tttaaggtcc gccagttcaa ccgggtagta aaaaatttat cagatcacta tatgctggtt
      961 gcggatgtgg agttgagaaa atagcttcag gttacaaata gtagtgggtg agcaacaaat
     1021 gcttttttat ttcatgaata agctcaataa ataatgtgct tgtagctcgc agcctgaagc
     1081 ttaaatttgc agctatgggt gacttaaaag tttataattc ctattcaaga caaaaagaaa
     1141 tctttacctc cattactccc ggtcatgtgg gcatgtatgt gtgcggacct actgtaagcg
     1201 gtgagagcca tcttggtcat gcccggccgt atattacgtt tgatgtgatc tatcgttacc
     1261 tgcaatacct cggttacaag gttcgctatg taaggaatat taccgatgca ggtcattttg
     1321 aggaagaagg aagggaagca gtagataaga ttgcaacaaa agccgtattg gaaaaactgg
     1381 aacccatgga acttgtgacc aaatacacca atctctttca ttgggcaatg gaacaattca
     1441 acacattgaa gccttctatt gaaccaaccg ctaccgggca tattgtagag cagattgaaa
     1501 tgatcaaaca gattatggag gcgggttatg cttatgaagt gaatggctcg gtatacttcg
     1561 atgtaaaaaa atatgcagcg ggccatgact atggcaaact aagcggaaga gtgatcgaag
     1621 accttcttga aacaaccagg gaactggaag gacaggagga aaaaagagac actgctgatt
     1681 ttgccctttg gaaaaatgca gcaccggaac atattatgcg ctggcaaagc ccctggggag
     1741 taggttatcc cggctggcat attgaatgct ctgccatggc caccaaatat ctcggggcgc
     1801 aatttgacat acatggcggc ggcatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga
     1861 aattgcgcag agtactatct gcaatcattc agcgccggtt cgttactgga tccataataa
     1921 catgatcacc attaacggcc gcaaaatggg taagagctat aacaatgtga tcaaactgac
     1981 agaactgttc agcggagacc acccgatact ggaaaaagcc tatcacccga tgacggtgcg
     2041 tttcttcata ttgcaaacac attaccgcag tactcttgat ttcagtaatg aagcattgca
     2101 ggcatcagag aaaggtttga aaagattgtg ggatgcatat gaaaagctgg ggaatttgtc
     2161 aatagatgac agtcaacagg ccatagatgc tgaactggat gaaaaaattc gaaaacaaat
     2221 tgccgagttt gatgaattta tgaacgatga tttcagtacg gctaaagtgc tggccaatat
     2281 gtttgagatc gttccggtga tcaactcaat gaaggataag atcattcctg tatcagccct
     2341 ttcgaaagaa acatttgaat tgctgcaaac cagttttaaa acatggttgg aagatatttt
     2401 tggattacaa tctgtcagcg aagcggataa tgccaaattg cagggagtga tgcagttgct
     2461 tattgacatc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2521 ctacgtccga taagattaga aaccaattgg ctgccctcgg cattacgctg aaggatgaga
     2581 agggagggga aatgagctgg agcgttgagt aatgaatcca gcaaagtatt ttgtcagccc
     2641 ggcagaggaa actgccgggc ttttcttttt ctcttaaaca aggtgacacc cgttttatca
     2701 caattcactc cccaaaatca acagttcact ccctgctgct tacccgcaag ctgtttggtg
     2761 ctgttatatt cttaaacaaa atcaaacaag tatgaaactc aaaaatctta tcagcaggac
     2821 cagtcttttc ctgttattgt cagcaccggt tgttttaatc agctgcgaca aagacgatga
     2881 taccgctact tctaacacta ttacagacat tgttgtggcc aatggtgatt tcagcaccct
     2941 ggaagcagca gtagtaaggg ccaatttgca gacaacatta agcggttcgg gaccattcac
     3001 tgtttttgca ccggataatg ctgccttttc agcagccgga attaccacat ctgtattaaa
     3061 ctcattgtct cctgcacagg tacaaagtat cctgctgtat cacacccttg gttcaagggt
     3121 aaatgcggct aatgtacccg caggacctaa tgctaaagta actaccgcca gcggagactc
     3181 tgtatttgta acccgtgatg caagaggtgt ttttgtaaat ggtatacagg ttacaacggc
     3241 tgatatcagc gctgataatg gggtgataca ccgtattggc cgtgtactga accctccggt
     3301 aggtaatatc gtggagactg ccgttgccag cggnnnnnnn nnnnattggt aaaagcagtt
     3361 acaagagcta ccacagcccc gggcggtgat ccaacgctgg ctggtactct gggttcagct
     3421 acccttacag tatttgctcc cactaatgca gcctttacac aattacttgg cgccctgagc
     3481 cttacagata ttaatcagat tcctatcggt acattactgg cagtactccg ctatcatgtg
     3541 gtaggcggaa gggccttctc ttctgatctt accaacggaa acctggccat gctggctggt
     3601 ggaaacacta ctgtaagcct gaccaccgga gctactatcc gtggcaacgg taatggcgga
     3661 aatgcctcaa atattaccgc gacgaacatt gtgtgccgga atggtgttgt acacctgatc
     3721 gacagggtgt tgttgccata actatatacc tggcgtaccc tgtatgaaaa acctcgtcgg
     3781 tcccgtctca gcagtggcgg gaccgattta tcaattaggg tcaaaaatgt tcaaccaccg
     3841 ctacagatat ctttttattg gcttgctggc cctgtatact ttcctgaata cagagctctg
     3901 caatgtttac cactattttg atattgagat cgagtggtat tatgcatttc tgaccatcct
     3961 gcttataaca ggatttacct gggaaggcaa ccgcttactg gagcctttta tacagcaaaa
     4021 agtacagcca caacgaaaca agtggagata cctcggcatc ttttttatag cgggtaacat
     4081 tgttgcttct ctcgccgcaa ttgctgtcgt atattttatt ggtaatgtat tgcatggtta
     4141 ctcctggcaa caaaatctaa atccgctgaa gctgaatata atttatgtca gtcttgtcaa
     4201 tttattcttc catcttgtga atgcgatatt ctttttcttc cgggaatacc gaacccagtg
     4261 gatggaagct gaagaactga gacgcagttc tacacaagcc gaactggaaa tggtaaaaag
     4321 ccagattaat cctcactttt tattcaataa cctcaatgtg ctttccggga tggtgataaa
     4381 agacaatccc gaagccaata aattcattga ggaattttcc aaagtgtacc gctatatcct
     4441 gagcaaccag caaaaagaac tggtggagct tcgctcagaa ttagacttta tccaacctta
     4501 tatatttctg ctgaaaaaac gatttgataa gggccttgta attgatatca atgtggcgga
     4561 ccggtacaaa aatttatatg ttgtgcctgc cgccctgcaa atgctgattg aaaatgcaat
     4621 caagcataat gtggtatcgc agcacaagcc tttgcatatt gatatacatt ccaacggtaa
     4681 tgaaacactg gtggtgaaga ataacctgca gccaagaact gtaaatgaac catcgagtcg
     4741 tattggtctg caaaatatac gccggcgtta tgagattgtt agcggccgtg aggtcgaaat
     4801 caaaaaatca acgaccgact ttgaagttac cttaccatta ttaaatctaa actgacagac
     4861 tatgaagctc atcattgtgg aagacgaaaa actggcagca gaacggttga aaacactggt
     4921 gaaggaatat gatccttcta ttgaagtgct ggcctgcctg gaaagtgtag aagaaaccgt
     4981 acagtatttg cgtcatcatg cagcgcctga cctgttgttg ctggatattc atctgtcaga
     5041 tggacatagt tttgaaatat tcagccaggt atattataaa ggaccggtaa tctttacaac
     5101 tgcctatgat caatatgctc ttgaggcctt caaaatgttc agcattgatt atatcctgaa
     5161 gccggttacc ctggaagcat tagcaacagc catgaataaa ttcagaaacc tgtcggccag
     5221 ttttgcctcg gctgattacc gtcaactaat gccccaactc agcagcaggg aatacaagaa
     5281 aagatttttg ggtaaggttg gccagcgttt gttttttgta gatactttag atattgccta
     5341 cttccaggca gataataaag tagtgtacct ggtggataag gaaggtaacc gctacgtggt
     5401 ggagcatact atggaaaagc taatggccat ggtaaaccct tcggaatttt tccggctaaa
     5461 ccggcggtat attgtccgta tcaatgctat acaacaggtg aagccttatt tcaacggaag
     5521 gctgaaactt tcagtaaaag gatcgcagca gccggaagag atggtgatca gccgcgaaag
     5581 ggtatctgag tttaagcaat gggccgaagc atgaagagac aggttgtaat attgtacaac
     5641 ctgtgaccac agccttagtt ttatctcctg accgccctta gcatttctct cttnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag gcccgctgca
     5761 catcaccttt gctgcaatag gtgaccaata atccgccttc tgccatcata ctgtacaggt
     5821 tttcaaaaat ttcttttgtc cataattcag gttgagctgc aggggcaaaa gcatcgtaat
     5881 aaactatact gtatacttcg tcggttgaga attcctgtaa agtggtatta tgttttgtta
     5941 gcctgaaata ctgggtgatt tcatacatct ctccccggcc gcactggtgc attttctcaa
     6001 acaactgctg gtattgcgac aggttcagtt gttcgcaata gttcagttgt gctattagag
     6061 gttcatccag cggaaaaggt tcaatggctg tatagtagat ccggttctta tgtctgtccg
     6121 cttcaatcaa tgtaagcaat gcattcagcc cggtgccaaa acctatttcc agcacctggt
     6181 gtacaccgat gtaatcaaaa atgccggaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     6361 ctgcaaaatt ctatatttat tattcaatct gaacacaagg atggaatacg tagctcattt
     6421 gtcgaaggac aaaaaactaa agagactgct ggaaagcagt gaactgcacc agttaaaaaa
     6481 gcggaagaat atttgcacct atctctgtgc ctccatcatg agccagcaac tcagtactaa
     6541 agtagctaca gtgatacata accgtttcct tgcattgtat ggtggtaaag agcctttgcc
     6601 gcaacagata ttggatactc cttttgagaa gttgaggagt atcggtttga gtaatgccaa
     6661 agcgaattat gtaaagaatg tagcacagtt tgaaattgat tttggcatgg atgcaaagag
     6721 gctgcacaaa atggacaatg aagaagtgat tgcttacctc acacagataa aaggtgtggg
     6781 ccggtggacc accgaaatgc tgctgatgtt tgcccttggc agggaagatg tatttgcggt
     6841 ggatgatctc ggcattcaga atgccatgat cagtttatac aaactggata acagccataa
     6901 aaagaaactg cgggaagata tgctccgcat atcacaaaaa tggagcccct accgcactta
     6961 tgcctgcctg cacctgtggc aatggaagga taatacacct gttaaaaaaa agcagtaata
     7021 aaaaacgaag cagcaattgt attccgcagg ttgtctgcac caataaaata aaggcatgaa
     7081 gcagatcaaa agttttacag tggtcttttc agtaatgttg ttgattttct ccggctcctg
     7141 ccagaaaagc agcgatgaat gtattgatga aagccggatg tctaacaatc cctgtaccct
     7201 ggagtacaat cctgtttgtg gttgtgatgg ggtaacttat tctaacccct gctttgcaga
     7261 cagggctggt gtaacccgaa ccacacccgg cgtttgtcct taaaatattg tagctttctc
     7321 acaaatcttt tctatgcaca aatggttgcc ggccctgctg ctgcctgtta tgttttcgtt
     7381 ttgctccggt aaacaaaggt ccgatgaatg gatttccctg tttgatggca aaacactcca
     7441 tggctggaag gtgggcgcca atgcttctgc atttaatgta gagaatggca gtattgttgt
     7501 caatggtaat gtgtcgcatt tgtattatga tggtaaagtg caacagcacc agtttaaaga
     7561 ctttgaattt aaagcggagg tgatgaccac tcccggttct aattccggga tctattttca
     7621 tacacaatac caggaaaaag actggccaaa g
//
LOCUS       DAORLS010000145         7618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5214_length_7618_cov_1.850192, whole genome shotgun sequence.
ACCESSION   DAORLS010000145 DAORLS010000000
VERSION     DAORLS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_7618_cov_1.850192"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK977_11485"
     CDS             <1..314
                     /locus_tag="PK977_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18786.1"
                     /translation="YKDHIRFTPQLSFTSGTQKFGFNQSSSTYATTVRTGTNVLFNSE
                     NVYLDDQIKFQPLSLTLFLRGEYSIGKFFIQPQLMLDYYFPANADNFNAIFQMNLGFI
                     F"
     gene            490..>689
                     /locus_tag="PK977_11490"
     CDS             490..>689
                     /locus_tag="PK977_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HRF18787.1"
                     /translation="MKTKFLLLFAAFFVSAASMAQFHLGVKGGANITKVDGKSFKDEF
                     RYGYHLGGFAEIRMGNKFVLQP"
     assembly_gap    690..722
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <723..1088
                     /locus_tag="PK977_11495"
     CDS             <723..1088
                     /locus_tag="PK977_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF18788.1"
                     /translation="ATKLDSSFKNVYQDVFDGNSNIKLNYISIPIVINYKLIGSFLSL
                     QAGPQFGILIDQSRTTLQNGADAFKKGDFSMLAGLNFKVGPMRINGRYAIGLNNISDI
                     TDDNKWNSQGFQVSVGLAL"
     gene            1308..>2426
                     /locus_tag="PK977_11500"
     CDS             1308..>2426
                     /locus_tag="PK977_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRF18789.1"
                     /translation="MRSEDEMDFIPIIPLNESDHDGVDGIEVPAEIPLLPLRNTVLFP
                     GVVLPITVGRDKSIKAVNEAYKGDKLVGVIAQKDTNIEDPSTKDLEDIGTVARIVKLI
                     KMPDGGTTIIIQGKSRFQVESILMDEPYFKAKIKKLEEEEAPKDEDFKAYVANIKDLA
                     ADIVQLSPNIPTEASIILRNIENPSFLIHFVSSNLNTDIKDKQRLLELNQIRQRADLL
                     MQLLQKELQFAELKNKVTTKTRTEIDKQQREYFLQQQLKSIKEELGGDSNSQEIKEMQ
                     KKAEAKKWPAAAKEMFKKGVEKLERMHPSTPDFSVVYNHLDLMLELPWEDYTEDIYDL
                     KKAKKTLDADHYGMQKIKERILEYLAVLKLKGDMKSPIL"
     assembly_gap    2427..2466
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2467..>2808
                     /locus_tag="PK977_11505"
     CDS             <2467..>2808
                     /locus_tag="PK977_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF18790.1"
                     /translation="LGKSIASAIGRKYVRLSLGGLHDESEIRGHRKTYIGAMPGRILQ
                     SIRKVKSSNPVMILDEIDKVGSDFRGDPSSALLEVLDPEQNNTFYDNYLELEYDLSKV
                     LFIATANNIQN"
     assembly_gap    2809..2829
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2830..>3129
                     /locus_tag="PK977_11510"
     CDS             <2830..>3129
                     /locus_tag="PK977_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRF18791.1"
                     /translation="IDLSGYAVEEKIEIAKRHLLPKQKEMHGLKSSHFKISDKMLEKI
                     IQDYTRESGVRELDRQLASVMRFQAKELALKGKLKANITANDIEKILGKPRYSND"
     assembly_gap    3130..3278
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3279..3679
                     /locus_tag="PK977_11515"
     CDS             <3279..3679
                     /locus_tag="PK977_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRF18792.1"
                     /translation="LTYLQANAKRYGINHTLFEKKNIHIHVPEGAVPKDGPSAGITMM
                     TAIASALTGKKVKPFLAMTGEITLRGQVLPVGGIKEKVLAAKRAGLKEIILCWQNEKD
                     VQEIESAFIKGIRFHFVKNMSQVLELALVE"
     gene            3818..4879
                     /gene="porQ"
                     /locus_tag="PK977_11520"
     CDS             3818..4879
                     /gene="porQ"
                     /locus_tag="PK977_11520"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HRF18793.1"
                     /translation="MKLIFLILTIFLNTSVFSQTLGGNSVFNFLKLANTPQLSALGGV
                     NVSGVSNDIGMSFNNPALLKSSMHSQMNAVFNSFYGDVKVYHLSLGYRNDKLNTNFSW
                     GLNYLAYGNTVQTDASGNELGSFRPVDWVMQLSASRKYLEKWNYGLTIKFINSNYGQF
                     RSSGIAADLGLLFHDTVNLFSASIVAKNMGTQLKRYNGTTADDLPFDLQIGFTKRLAK
                     APLAFSLTAQRIHRFDILYNDTLFNNENGFENARQNKFTIDKLINHLIIGTTIYAGDR
                     VEVQAGYNFLRRKELNIGNLSNGLNGFSIGAGVLFGKLQVRYARAYYQSNTAYNQFGL
                     NMQLNQYFGLGNFGKRIGW"
     gene            complement(4882..5514)
                     /locus_tag="PK977_11525"
     CDS             complement(4882..5514)
                     /locus_tag="PK977_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRF18794.1"
                     /translation="MLTVNKILVLAPHTDDGELGCGGTIAKFCNEGKEVFYAAFCLCS
                     KSLPESLSANTLETECKNATSRLGIPASNLILFNYEVRELPASRQQILEELLQLNKQI
                     RPDLVLLPAASDVHQDHQVIHQEGLRAFKNTSFAGYELPWNNYSFRTNFFMKLTEQEI
                     DKKIEALSQYRSQSHRNYMNPDFIRSLAKVRGVQCDNSYAEAFEIYKLIS"
     assembly_gap    5670..5702
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5703..6646)
                     /locus_tag="PK977_11530"
     CDS             complement(<5703..6646)
                     /locus_tag="PK977_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18795.1"
                     /translation="MTIYLNTLFFVLITLDSFSLAISRPKEQNTNAVFNFTPCDSCQK
                     PDIYLIVADGYPGNRQLRDLFDFDNSNFENALKQKGFFIVDSSMSNYNFTQYSIASML
                     SLNYLKGIKHINSNKDDIQISHNALKNSPVISYFQNEGYTFFNHSIFDFKGQPSITIP
                     TFLTRKLEPITRQTLTYRIMKDLGYHFVTTLKIPFLVKRQLTADKVNNQKIIAATQKT
                     IHQKNNKQKFVYTHLMMPHHPFFYDSTGKEVPLELLKDEFFFDKGARISYLKYTNNVL
                     LKLIDEILSYNKEKPVIFLISDHGIREIRDKFDPHYQF"
     assembly_gap    6671..6760
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6761..7041)
                     /locus_tag="PK977_11535"
     CDS             complement(<6761..7041)
                     /locus_tag="PK977_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18796.1"
                     /translation="MNTSLKQFLERNVLFVILLPILWVLHGYTNNLGLVPKKDALLLA
                     LFYCSVALILTGILWLFYRNIFKAALLTFLLLSLQFLFGPVHDFLKGSFP"
     gene            complement(7038..>7618)
                     /locus_tag="PK977_11540"
     CDS             complement(7038..>7618)
                     /locus_tag="PK977_11540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF18797.1"
                     /translation="LAEKKRITTEWLDKMDSVKTAADLGANNGEFSQMLAERRISTVA
                     ADFDPACINSLYNNIKNKEIQNIIPLAVDLSNPSPSIGFNNEERPSFINRSAFDLTIA
                     LALIHHLAISKNIPIQKLAELFKRITNKYLIIEFVPKEDEKVQLLLNNRKDIFFNYNA
                     NNFELSFETNFRIVAKELIGESGRILYLMEKK"
BASE COUNT         2260 a         1300 c         1501 g         2191 t
ORIGIN      
        1 cctataagga ccatatccga tttacgcctc aactttcttt tacaagcggt acccagaaat
       61 tcggtttcaa ccagtcctcc agtacctatg ctactactgt cagaaccggc accaacgttc
      121 tttttaacag tgaaaacgtt tacctggatg atcaaataaa gtttcagcca ctttcgctca
      181 cactgtttct gaggggtgaa tactcaattg gaaaattttt cattcagccc cagctaatgc
      241 tggactatta tttcccggca aatgcagata atttcaatgc catttttcag atgaaccttg
      301 ggtttatttt ttagcagcag attccttaat aaaatcttca cagatcttat acaatagccg
      361 gttacaattt ttgtttttga aattacctat tagtcgttaa tggaatttaa caagccattg
      421 gtttgtatgg cattatactt gaaacttttc ggggtcaaat ttcactaacc atttttaaaa
      481 agtatacaca tgaaaacaaa attccttttg ctcttcgcag ctttttttgt atccgccgcc
      541 agtatggccc aattccatct tggggttaaa gggggcgcca atattaccaa ggtggatggt
      601 aaatcgttta aagatgagtt ccgttacggc taccacctgg gtggctttgc agaaataaga
      661 atgggaaata aattcgtatt gcagccggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nngccacaaa actggacagc agctttaaga atgtttacca ggatgttttt gatggaaaca
      781 gcaatatcaa actgaattat atttctattc cgatcgtaat taactacaaa ctgataggca
      841 gttttctcag tttgcaggca ggccctcagt ttggcatcct tatcgatcag agcaggacta
      901 cactacagaa tggagctgat gctttcaaga aaggggattt ctccatgctg gcaggcctga
      961 acttcaaagt gggccctatg cgcatcaacg gccgctatgc aatcggcctg aataacatca
     1021 gtgatataac cgatgataat aaatggaata gccagggatt ccaggtttcc gttggattag
     1081 ctctttaaac ataatctcag ccatgaataa ctaaggccct ccggacagga gggctttttt
     1141 atgctatcta ttcatcctca attacataat ttggcagttt ttacggccgg gcatgaatct
     1201 tggcaatact gttttcccga tttactttta acccgcatcc tgttacagta gtggcagggt
     1261 gacaatatgt ctaaagcagc attgaaatga gtatagaaaa atatttaatg agatctgagg
     1321 atgagatgga ctttattcct ataattccct tgaatgaaag tgatcatgat ggagtagatg
     1381 gcatagaagt ccctgcagaa attccgcttt tgcctttaag aaatactgtt ctttttcccg
     1441 gtgttgtatt gccgataacc gtaggcaggg ataaaagcat aaaagccgta aatgaggctt
     1501 ataaaggcga taaactggtt ggtgttattg cacaaaaaga taccaatata gaagacccct
     1561 cgaccaaaga ccttgaggat attggcacag tggcacggat tgttaagctg attaagatgc
     1621 cagatggagg gactactatt attatccagg gcaaaagtcg cttccaggtt gaatccatat
     1681 tgatggatga accttatttc aaagcaaaaa ttaaaaaact ggaagaggaa gaagcgccaa
     1741 aagacgaaga tttcaaagct tatgtagcca atatcaaaga tctggctgca gatattgtac
     1801 agttgtcacc taatattccc acggaagctt caattatact tcgcaatatt gaaaaccctt
     1861 ctttcctgat tcattttgtt tccagcaacc tgaatactga cattaaggac aaacaacgac
     1921 tgctggaact gaaccagatc aggcaacgag ctgatctgct gatgcagcta ttacagaaag
     1981 aattacaatt cgctgagctt aagaataaag taactaccaa aacaaggaca gaaattgaca
     2041 agcagcaaag agagtatttc ctgcagcagc aactgaaaag tataaaagaa gaattaggcg
     2101 gtgattcgaa ttctcaggaa ataaaagaaa tgcagaaaaa ggcggaagca aaaaaatggc
     2161 ctgctgcagc aaaggagatg tttaagaaag gagtagagaa actggagcga atgcatccca
     2221 gcacaccgga tttttcggtg gtatataatc acctggacct gatgcttgaa ctgccatggg
     2281 aagattatac agaagatatt tacgatctga aaaaagcaaa gaagacatta gatgccgatc
     2341 attatggcat gcaaaaaatt aaagaacgaa tattggaata cctggcagta ctgaagctaa
     2401 aaggtgacat gaagagcccg atacttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnatta ggaaaaagta ttgccagtgc tatcggcaga aaatatgtaa ggctgagtct
     2521 tggaggtctg catgatgaaa gcgaaataag ggggcataga aagacttata taggtgcaat
     2581 gcctggcagg attttgcagt caatacgtaa agtaaaatct tcaaacccgg taatgatact
     2641 ggatgaaata gataaagtgg gcagtgactt tcgcggagat ccatcctctg ctttgctgga
     2701 agtattggat ccggaacaga acaatacttt ctatgacaat tacctcgaac tggagtatga
     2761 cctgagcaaa gtattgttta ttgcaacagc taataatatt cagaatatnn nnnnnnnnnn
     2821 nnnnnnnnna atcgatctca gtggttatgc cgttgaagaa aaaattgaaa ttgccaagcg
     2881 ccatctttta ccaaagcaga aagagatgca tggtctgaag agcagccatt tcaaaatctc
     2941 ggataaaatg ctggagaaga ttatccagga ttatacccgg gaaagtggtg taagagaact
     3001 ggacaggcaa ttggcatcgg taatgagatt ccaggccaaa gagctggcac taaagggaaa
     3061 gcttaaagca aacattacgg ctaacgatat tgaaaaaata ttaggcaagc cccgttatag
     3121 taatgatttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ttaacttatt tgcaggccaa
     3301 tgctaagcgt tatggcatta atcatacttt gtttgagaaa aagaatattc acattcatgt
     3361 gcctgaaggg gcagttccca aagatggtcc cagtgcaggt ataaccatga tgacagccat
     3421 cgcctccgca ctgactggca aaaaggtcaa gcctttcctg gcgatgacag gagaaataac
     3481 cctcaggggt caggtattgc ccgttggcgg aattaaggag aaagtattgg ctgctaaaag
     3541 agccggactc aaggagataa ttctttgctg gcaaaatgaa aaggatgtcc aggaaataga
     3601 gtctgccttt ataaagggaa tccggtttca ctttgtaaaa aacatgagcc aggtgctgga
     3661 attggcgctt gtagaataaa tattaatgtt attcaaccaa ctagcaggaa tcttgttata
     3721 tctgtacatg ttggttgttt actaattacc gttgaggtga gaagatcccc ctgttgctgg
     3781 gggattttct tttggtaaag gcctaatttc gttgcctgtg aagcttatct ttctaatact
     3841 gactattttc ctaaacacaa gtgtcttttc acaaacactt ggaggcaatt ctgtatttaa
     3901 ttttctaaaa ctagccaata cacctcaact atcagcatta ggaggagtga atgtctccgg
     3961 tgtgtccaat gatattggaa tgagttttaa taacccggcc ttgttaaaat cttccatgca
     4021 ttcacagatg aatgccgtat ttaatagttt ctatggagac gtaaaggttt atcatttgtc
     4081 acttgggtat cgcaatgata agttgaatac taattttagc tggggtctga attatctagc
     4141 ttatgggaat actgttcaga cagatgcttc gggtaatgaa ttgggtagtt tcagaccggt
     4201 agattgggta atgcaattat ctgcatcaag aaaatacctt gaaaaatgga actacggtct
     4261 taccattaag tttatcaatt caaactacgg tcaattcagg tcaagtggca ttgcggctga
     4321 cttgggtttg ctttttcatg ataccgtaaa tttattttct gcatccatcg tagcaaaaaa
     4381 tatgggtact cagttgaagc gatacaatgg cactacggcg gatgatctgc cttttgacct
     4441 tcagattggg tttacaaaaa ggttagctaa agcgccgtta gctttttctc ttacggctca
     4501 gcgaatacac cggtttgata tcctttataa tgatacctta ttcaataatg aaaacgggtt
     4561 tgagaatgcg aggcaaaata aatttactat agataaactg atcaatcatt taatcattgg
     4621 caccaccatt tatgccggtg acagggtaga agtacaagcc ggatataact tcttacgaag
     4681 aaaggagttg aatatcggta atctatcgaa tggactaaac ggtttttcca tcggtgcagg
     4741 ggtgctcttt ggtaaattac aggttcgcta tgcaagggct tattaccaga gtaacactgc
     4801 ttataatcaa tttgggttga atatgcagtt aaaccaatat tttggtctgg gaaattttgg
     4861 taaacgaatt ggctggtagg cttagctgat cagtttatat atttcaaatg cttctgcata
     4921 agaattgtcg cattgaactc ctcttacttt tgccaatgag cggataaaat ccgggttcat
     4981 ataatttcta tgtgattgcg aacgatattg actcagcgct tcaattttct tatcaatttc
     5041 ctgttcggta agtttcataa aaaagttggt ccggaaactg taattattcc agggtagttc
     5101 atagcctgca aaactggtat tcttaaatgc cctcaagccc tcctggtgaa tgacctggtg
     5161 gtcctggtgt acatcggatg cggcgggtaa taaaaccaga tccggccgga tttgtttatt
     5221 tagttgtagt aattcctcca gtatttgttg ccttgatgct ggaagttccc ttacttcata
     5281 attaaacaaa atcaggttgg aggctggaat gccaagtctt gaagtagcat ttttgcattc
     5341 ggtttccaat gtatttgctg ataacgattc aggcagggat ttggagcaaa ggcaaaaggc
     5401 agcataaaac acttcttttc cttcattgca gaattttgca attgttccac cgcaacccaa
     5461 ttcaccatcg tcagtgtgtg gggcaagaac cagtatttta tttactgtaa gcatttctca
     5521 aaaataaact tatgttttca tattcttaat ccgtaataaa tattgtagag tctttcagca
     5581 taggtaaatt ttgccggaac tgtttattaa ggaagaatct gaaaaaatta atgtttgaca
     5641 ttcctttgta gagcccatca taatctctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnatgaattg ataatgcggg tcaaatttgt cccggatctc tctgatgccg tgatcgctaa
     5761 taagaaaaat aactggcttc tctttattgt atgatagtat ttcatcaatt agctttaata
     5821 atacattgtt ggtgtatttc aggtagctga tcctggctcc tttatcaaag aagaattcat
     5881 cttttagtaa ttccagtggt acttctttgc cagtactatc ataaaaaaag ggatgatgcg
     5941 gcatcatcag gtgagtgtat acaaactttt gtttattatt tttttgatga atcgtttttt
     6001 gagttgcagc aattattttt tgattgttca ccttgtcagc agttaactgc ctttttacca
     6061 ggaagggtat ctttaaagta gtgacaaaat ggtagccaag atctttcatt attctataag
     6121 tgagagtttg cctggtgata ggttcgagtt tccgggtaag aaatgtagga atggtaatcg
     6181 agggttgtcc tttaaaatca aaaattgaat ggttaaaaaa agtataccct tcgttttgga
     6241 aataggaaat gaccgggctg ttttttaaag cattatggct tatttgaata tcatccttgt
     6301 tgctgttaat atgtttgatc cctttcaaat agtttagcga taacattgag gcaattgagt
     6361 attgtgtgaa attataattg ctcatactgc tatcaacaat aaaaaatcct ttttgcttta
     6421 atgcattctc aaaattgcta ttgtcaaaat caaataaatc cctcagttgt ctgttgccag
     6481 gatagccatc tgcaacaatc aagtaaatat caggtttctg gcacgaatca catggggtaa
     6541 aattgaatac tgcgtttgta ttttgttcct tgggcctgct gatcgcaaga gaaaaactat
     6601 ccaaagttat cagtacaaaa aaaagagtat tcagatagat agtcatccgg tgaaaattat
     6661 tctttgacct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaaatgatc ctttcagaaa
     6781 atcatgaacc ggcccaaaaa ggaactgtaa ggataaaagt aagaacgtca gcaaagcggc
     6841 tttaaagatg ttcctgtaaa ataaccaaag aattcctgtc aggattagcg ctacactgca
     6901 gtaaaacagg gcaagcagca atgcatcctt tttggggact aatcctaaat tatttgtata
     6961 gccatgtaaa acccacagaa tagggagaag aattacaaat aacacattcc tttccaggaa
     7021 ttgttttaga gatgtattca ttttttctcc attaggtata atattcgccc agattctcca
     7081 atcagctctt tagcaacaat acgaaaatta gtttcaaaag acagttcaaa attattagcg
     7141 ttatagttga agaagatatc ttttctgttg ttaagcaaca actgcacttt ctcatcctct
     7201 tttggtacaa actcaataat aaggtacttg tttgttatac gtttgaaaag ttcagccaat
     7261 ttttggattg ggatattctt tgaaatggca agatggtgaa taagggctag tgcaatcgta
     7321 aggtcaaatg cactgcgatt tataaacgat ggccgctctt cattattaaa tcctatcgaa
     7381 ggcgaaggat ttgataaatc aactgccaaa ggaatgatat tttgaatttc tttgtttttg
     7441 atattgttat agagactgtt gatacaagcc ggatcgaaat cagctgcaac ggtacttatt
     7501 cttctttccg ccaacatttg tgaaaattca ccattgtttg ccccaagatc agccgctgtc
     7561 ttaactgaat ccattttatc gagccattcg gtggttattc ttttcttttc agccaggt
//
LOCUS       DAORLS010000146         7614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5221_length_7614_cov_1.999339, whole genome shotgun sequence.
ACCESSION   DAORLS010000146 DAORLS010000000
VERSION     DAORLS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5221_length_7614_cov_1.999339"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PK977_11545"
     CDS             complement(<1..587)
                     /locus_tag="PK977_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF18798.1"
                     /translation="MRNIIVVLLAISLFACSSKKTETAAKEEEPADKITLTDEQLKNA
                     GVETGLVQKQLLNSELKVNGLVDVPPQNIVSVSFPLGGYLKTTRLLPGMHVNKGEIIG
                     IMEDQSLVQLQQDYLVAVVRLQYLEKEFERQKLLNENNVNADKVYQQAQADYTSQKVL
                     VKGYSEKLQLININPGKLTENNISRSVPVYSPING"
     gene            complement(594..>1288)
                     /locus_tag="PK977_11550"
     CDS             complement(594..>1288)
                     /locus_tag="PK977_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF18799.1"
                     /translation="QQTDADILIAQQYLQWILNINELILPSYVTVKRQASAGLADTSA
                     VSQHPQVQYIRLLEKTTAAQTAVEKSRLSPDVPIGYNNMSIIGYQSSDGVNQKYYGAG
                     DRFNIFSLSLGLPIFNKATKARIKAGQLNEQVSKLVTESATLQLKTKLQQAAEEFRKQ
                     ERNLHYYEQTGIQQSELIIKNAKLAFEKGEISYLEWSILMNNAVNIQLGHLQTLFQYN
                     IALIELEYLTGK"
     assembly_gap    1289..1447
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1448..1795)
                     /locus_tag="PK977_11555"
     CDS             complement(<1448..1795)
                     /locus_tag="PK977_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18800.1"
                     /translation="MLLSQVSVAQRKVTLEEVLKMATEKNLSLQSARMETGYWKQLQS
                     GVFDPAKTVIGTEYGNINSFNNDNRFFISQTFGMPVVYRRQKDWYLSNEIAQQQMVNW
                     KEAELRREVKKVFY"
     assembly_gap    1825..1853
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1854..>2847)
                     /locus_tag="PK977_11560"
     CDS             complement(<1854..>2847)
                     /locus_tag="PK977_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF18801.1"
                     /translation="ANKMAGIINTVQGAKDIYVEVVTGLPQIVVKYNREAMAHYQLNI
                     ADVNQVIRTAFAGESAGLVYENERRYDLVVRMKDEVRQDLTDVQQLLIPTPNGLQVPL
                     YQVATVEIKEGPNQIQREDAKRRIIVGFNVRGRDVESVVKELSEKEKKQLGLPSGYYV
                     KYGGQFENLVEAKQRLSLAVPAALLLILVMLYFAFGSLKYGILIFTAIPLSAIGGVLA
                     LWTRGMPFSISAGVGFIALFGVAVLNGIVLIAEFNRLKQHGLNDIKHIVMEGTQIRLR
                     PVLMTAAVASLGFMPMALSNGPGAEVQRPLATVVIGGLITATFLTLVVLPILYTW"
     assembly_gap    2848..2889
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2890..3091)
                     /locus_tag="PK977_11565"
     CDS             complement(<2890..3091)
                     /locus_tag="PK977_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF18802.1"
                     /translation="MTDIIITLKPKGQWTSASSYDELANKMSKALQEIPGMTSGFQFP
                     VQMRFNELISGARQDIVCKIFGE"
     gene            complement(3108..>4214)
                     /locus_tag="PK977_11570"
     CDS             complement(3108..>4214)
                     /locus_tag="PK977_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF18803.1"
                     /translation="RYTDAGIPLLIKDVAEVRIGHAIRYGAMTFSDKGEVAGAVVLML
                     KGANAEQVVHNVKQRIEQIKKTLPEGVILETFYDRTKMVDNAIGTVKTNLIEGALIVI
                     FVLVIFLGNLRAGFIVASVIPLSMLFAVILMNVFGVSGNLMSLGALDFGLIVDGAVII
                     VEAVLHRLSHNKHFAGLTHITQPQMNDEVESSAGKMMNSAVFGQIIILIVYLPILTLT
                     GIEGKMFKPMAQTVSFALIGAFILSITYVPMMSSLFLSKRLSHKDTWADKMMKFLQKL
                     YKPALELALRFPKMVVGSVLLLFAVALLIMSRLGGEFIPKLQEGDFAVDARILTGSSL
                     TETIKTTQKAVAILEARFPEVEDCYQGRCQRNSN"
     assembly_gap    4215..4229
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4230..4713)
                     /locus_tag="PK977_11575"
     CDS             complement(<4230..4713)
                     /locus_tag="PK977_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF18804.1"
                     /translation="MVTVVFNDHTDIYWARQQVSERLGQVKDQIPATAGRPELAPATT
                     GLGEIYQYVLRPKKGYETKYDLTELRSIQDWIVRRQLLGTPGVADVSSFGGYLKQYEI
                     SVLPERLKSMNVTIADVFLALEKNNQNSGGAYIEKGPTSLFIRTEGLATTTEEIGKIP
                     I"
     assembly_gap    4737..4823
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4824..4982)
                     /locus_tag="PK977_11580"
     CDS             complement(<4824..4982)
                     /locus_tag="PK977_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF18805.1"
                     /translation="MLNTTIRFSVRNKLIIGLLTIGLIIWGAIDLRKLPIDALPDITS
                     NQVQVITVS"
     gene            complement(5022..5405)
                     /locus_tag="PK977_11585"
     CDS             complement(5022..5405)
                     /locus_tag="PK977_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18806.1"
                     /translation="MRKLTGILLISVYMLSFAELHNLLKIPVLLEHFKEHKQEDPSIS
                     FWSFIKLHYFDPIVVDDDYQRDQQLPFRDTDCCIVTTASVCECLQVSVEIQPLSEQSK
                     EFYLFNEINKPQFTSFDIFQPPRCA"
     gene            complement(5458..>6055)
                     /locus_tag="PK977_11590"
     CDS             complement(5458..>6055)
                     /locus_tag="PK977_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18807.1"
                     /translation="NKIIYKNLDGFSGSKGVSLNIELNHANRLKGLFGITLQEVKKTE
                     NDPRGNKTTIRPVLTERWSGTWSITYSIPSIAFTIDYTGNVYGPMRLPLLSATDPRPE
                     FSPVWSIQNIQFSKIINRQFELFAGLKNLLNWTPAKNVPFLIARSHDPFDKNVAYNTD
                     GTVKATAENPFALTFDPSYVFAPNQGIRLFAGMRLTIK"
     assembly_gap    6056..6075
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6076..>7614)
                     /locus_tag="PK977_11595"
     CDS             complement(<6076..>7614)
                     /locus_tag="PK977_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF18808.1"
                     /translation="SVMAEDSSVYTISDINGNFSIATKEGAVLQISSTGFERKKVKVG
                     ENDFILVPVSPLQVELDAIVVTGTMKAVRRSESPVAVEVYTPHFLKKNPSPSIFESLQ
                     NVNGVRPQLNCSVCNTGDIHINGLEGPYTMITIDGMPIVSSLASVYGLFGIPTQLIER
                     VEIVKGPASGLYGSEAIGGLINIITKSPDKAPRFTANIMTTSWLEHNFDAGIRFKAGK
                     KINSLMGVNYFNYQHPFDRNNDQFTDIALQHRVSVFSKTNFSRKYNRSASLAGRYFYE
                     DRWGGDMNWNRSFRGTDSVYGESIYTSRWELIGNYQLPLSEKIIASFSATGHQQNSYY
                     GTTPYMGDQKIAFAQLIWDKVVGNTHSFLAGITNRYNYYDDNSTATLDTLTGINRPDK
                     TFIPGIFLQDEWKPGAKHLFLMGLRYDHHPVHNGIFTPRFAWKWSVKDGQVFRLNAGT
                     GFRVVSLFTEEHAALTGARAVDVQESLRPERSYNVNLNYTWYKKWKQLGLNLDASVWY
                     SHFMN"
BASE COUNT         1985 a         1687 c         1361 g         2229 t
ORIGIN      
        1 aatccattga tgggcgaata cactggtaca ctacgggaaa tattattctc agtcagtttg
       61 ccaggattaa tatttatcag ttgtaatttt tcactgtaac ccttgaccaa tactttttgc
      121 gaagtatagt cggcctgtgc ctgctggtag accttatctg cgttcacatt gttttcatta
      181 agcaattttt gcctttcgaa ttctttttcc aggtattgta atctcactac tgccaccagg
      241 taatcctgct gtaattgtac cagtgactga tcttccatta ttcctattat ttcgccttta
      301 ttaacatgca tacccggcaa caatcttgtc gttttcaggt aaccacccaa aggaaaactg
      361 acagacacaa tattctgcgg aggaacatct accagtccgt ttactttgag ttcactgtta
      421 agtaactgtt tttgcacaag tccggtctct acaccggcat ttttcaactg ttcatctgtg
      481 agtgttatct tatctgccgg ttcttcttct ttcgctgcag tttctgtctt tttggaagag
      541 caggcaaaca acgaaattgc cagcagtact actatgatat tgcgcataat attttatttt
      601 ccggttaaat attctaattc aatcagggct atattgtact gaaataaagt ttgcagatga
      661 cccaactgta tattgactgc attgttcatc agtattgacc attccaggta gctgatctct
      721 cctttttcaa aagcaagctt tgcatttttt atgatcagtt ccgattgctg aattcctgtc
      781 tgttcataat aatgcaggtt tctttcctgc tttctgaatt cttcagcagc ctgctgcaat
      841 tttgttttca actgtaatgt ggcagactcc gtcaccagtt tactcacctg ctcattgagt
      901 tgccccgcct taatccttgc ttttgttgct ttattgaaaa tgggaagtcc taatgataaa
      961 ctgaaaatat tgaatctgtc gccggcccca tagtatttct ggtttactcc atccgaactt
     1021 tggtaaccta taatcgacat attgttataa ccaattggta catcggggct caatcttgat
     1081 ttctcaactg ctgtttgagc cgctgtagtt ttctccagca accggatgta ttgaacctgc
     1141 ggatgctgac taacggctga tgtatccgca agtcctgccg atgcttgtct tttgacagtt
     1201 acataagacg gaagtatcaa ttcgtttata ttcaatatcc attgcaggta ctgttgggca
     1261 atcaagatat ctgcatctgt ttgctgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnata gaatactttt tttacttccc tgcgtaactc tgcttctttc cagtttacca
     1501 tttgctgctg tgctatttca ttcgacaggt accagtcttt ctgcctgcga taaacaaccg
     1561 gcataccaaa agtctggctg ataaagaaac ggttatcgtt attaaaactg ttgatattac
     1621 cgtattcagt accgatcact gttttagcag ggtcaaaaac tccgctttgt aattgtttcc
     1681 agtatccggt ttccatcctg gcactttgta gtgaaaggtt cttttccgtt gccattttca
     1741 gtacttcttc caatgtcact ttccgttgtg caactgaaac ctgtgaaagc agcatgctta
     1801 gaatcaaaat tgttgcagct ggttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaccatgt
     1861 gtatagtata ggaagtacta ccagggttaa aaaagttgct gttatcaatc ccccgattac
     1921 aacagtagcc aatggccttt gcacttcagc accgggacca ttactcaatg ccatcggcat
     1981 gaagcctaaa gatgcaacgg cagctgtcat cagcaccggc ctcaaacgaa tttgtgttcc
     2041 ttccataaca atgtgtttaa tatcatttaa tccatgttgt tttaaccggt taaattctgc
     2101 tatcaataca ataccgttta atacagccac accaaataag gcgataaatc caacacctgc
     2161 tgatatacta aagggcattc ctcttgtcca tagtgctaac acaccaccaa tagcggacaa
     2221 aggaatggcg gtgaaaatga ggataccata tttcaaagaa ccaaaagcga aatacagcat
     2281 aaccaatatc agcaacaagg cggcaggaac agcgagtgat aaacgctgct tcgcttctac
     2341 caggttttca aattgtcctc catactttac ataatagcca gaaggcagac ccagttgttt
     2401 cttttctttt tcacttaact cttttacgac cgattcaaca tcccttcccc ttacgttgaa
     2461 gccgacaata atccttcgtt ttgcatcctc ccgttgtatc tggttgggac cttcctttat
     2521 ttccactgta gccacctggt acaaaggaac ctgcaaacca ttcggcgttg gtatcagcaa
     2581 ttgctgcaca tcggttaaat cctgccttac ttcatctttc atccttacca caaggtcata
     2641 tctcctttca ttttcgtata cgaggccggc actttcaccg gcgaaagcag ttcggataac
     2701 ctgattgaca tctgcaatat tcaactggta atgcgccatg gcctccctgt tatatttcac
     2761 tacgatctgg ggcagtcccg ttaccacttc tacataaata tctttagccc cctgcacagt
     2821 attgatgata cctgccatct tattagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnc ttctccgaaa atcttacaaa cgatatcctg tctcgcaccg gatatcagtt
     2941 cattaaaccg catctgcacc gggaactgaa aaccacttgt catacccgga atttcctgta
     3001 aggccttact catcttgttc gccagttcat catagctgga agcagatgtc cattgacctt
     3061 tgggttttaa agtgataatg atatctgtca tttccaccgg catggggtca gttggaattt
     3121 cgctggcacc taccctggta acaatcttcg acttcaggaa aacgagcttc caaaattgcg
     3181 actgcttttt gtgtggtctt tattgtttcc gttaatgaac tgcccgtaag tattcttgca
     3241 tccactgcaa aatccccttc ctgtagtttg ggaataaact ctccccctaa acggctcata
     3301 ataagaagtg caactgcaaa taaaagcaga accgatccaa ccaccatttt aggaaaccta
     3361 agtgccagct ccagtgccgg tttgtaaagc ttttgcagga atttcatcat cttgtcggcc
     3421 catgtgtctt tatggctcag tcttttactc aggaacagtg atgacatcat tggtacataa
     3481 gtaatggata aaatgaaagc accaatcagt gcaaatgata ctgtttgtgc catcggctta
     3541 aacatttttc cttcaatacc tgtcagtgtc aggataggca ggtacacaat gagtataatg
     3601 atctggccaa atactgccga gttcatcatc ttacctgctg agctttctac ttcatcattc
     3661 atttgcggtt gtgtgatatg tgtaagacca gcaaaatgtt tattgtgcga aagccggtgc
     3721 aacactgctt ccacgataat gacagcgccg tctacgatca atccgaaatc cagtgcgcca
     3781 aggctcatca ggtttccgga tacaccaaat acattcatca gtatcactgc aaaaagcatg
     3841 gaaagcggaa taacagaagc tacaatgaat cctgcccgca ggtttcccag gaaaatcacc
     3901 aatacaaaga tgacaatgag tgccccttca attaaattgg tcttcactgt accaatagca
     3961 ttatccacca ttttggtacg gtcatagaac gtttccagga taactccttc cggtaatgtt
     4021 tttttgatct gctcaatgcg ttgcttgaca ttatgcacta cctgctcagc attagctcct
     4081 ttcagcatta aaactacagc gccagctact tctcctttat cgctaaaggt cattgcaccg
     4141 taacgaatgg catgaccaat ccgaacctca gccacatctt ttatcaggag cggaatgcct
     4201 gcatcggtat aacgnnnnnn nnnnnnnnng tatgggtatt ttcccaattt cttcagtagt
     4261 agtggctaaa ccttccgtac gtataaaaag agatgtcggc cctttttcaa tataagcgcc
     4321 accggaattc tggttatttt tttccaatgc caaaaaaaca tcagcaatgg taacgttcat
     4381 actctttagc ctctccggta aaactgatat ttcatattgt ttcagatagc cgccaaaact
     4441 gctgacatcg gccactcccg gagtgcccaa taattgtctt cttactatcc agtcctgtat
     4501 cgacctgagt tcagtcagat catactttgt ttcatatccc ttttttgggc gaagtacata
     4561 ttgatatatt tctcccaaac cagttgtggc cggcgccagt tctggtcttc ctgctgtagc
     4621 cggaatctgg tcttttacct ggcccagcct ttctgacacc tgttgtcttg cccaatagat
     4681 atcagtgtga tcattaaaaa ctacagtaac cactgataac ccaaaacggg atattgnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnncgagaca gttattacct gtacctggtt actggtaata
     4861 tcaggtaaag cgtctatagg taattttctg aggtctattg ctccccatat aatcaatcct
     4921 atggtcaata aaccgatgat aagcttgttc cttacggaaa agcgaatagt tgtatttagc
     4981 atgaaaataa tttgtgcctt aaaaaatcag atcatatcgt ttcaggcaca acggggaggc
     5041 tggaaaatat cgaaagaagt gaattgaggt ttattaatct cattaaagag gtaaaactcc
     5101 ttcgactgtt cagataatgg ctgaatttct actgacactt gcaggcactc acaaacactg
     5161 gcagtagtga caatacaaca atctgtatcc ctgaagggca attgctgatc ccgctgataa
     5221 tcgtcatcta ccacaatagg gtcgaagtaa tgcagcttaa taaaagacca aaaactgatg
     5281 gaaggatctt cctgcttgtg ctctttaaaa tgctccagca atacgggtat cttcagcagg
     5341 ttatgcagct ctgcaaaaga cagcatgtaa acagaaatca gcaatatgcc ggttaatttt
     5401 cgcaccgggt aaagatacaa aactgatttt ttatcccggt gacaaatatc acggccctta
     5461 tttaatggtc aatctcatcc ctgcaaacaa cctgattccc tggttaggtg caaacacata
     5521 tgatggatca aatgtaagag caaatggatt ctccgcagtc gccttaactg tgccatcagt
     5581 attatacgct acgtttttat caaaaggatc atgtgaacgg gcaatcagga aaggtacatt
     5641 ctttgccggt gtccagttca gcagattttt taacccggca aacaactcaa attgcctgtt
     5701 gatgattttg ctgaactgaa tattctgtat gctccagaca ggagaaaact ccggtcttgg
     5761 atcagtggca gataataggg gcagccgcat ggggccatac acattacctg tgtagtcaat
     5821 tgtaaaagca atagaaggaa tagaataggt aattgaccat gtgccactcc atcgttctgt
     5881 caacacaggc cggatagttg ttttattccc ccgggggtca ttctctgttt tctttacttc
     5941 ctgcaaggtt ataccgaata aacctttcag ccggttggca tggtttagct cgatattcaa
     6001 ggaaacacct tttgaaccgg aaaatccatc caggttttta taaatgattt tattgnnnnn
     6061 nnnnnnnnnn nnnnnattca taaaatgtga ataccaaacc gatgcatcaa gattaagtcc
     6121 taattgtttc cactttttat accatgtgta attaaggttc acattatagc ttctttccgg
     6181 ccttaatgat tcctgtacat ctacggccct tgcaccagtt agtgctgcat gttcttctgt
     6241 aaataagctt accaccctga aacctgttcc tgcattcagc cgaaaaacct gtccgtcttt
     6301 gacagaccat ttccatgcaa aacggggagt aaagatgcca ttatgaacag gatgatggtc
     6361 ataacgaagc cccatcagaa acaggtgctt tgctccgggt ttccattcgt cctgcaaaaa
     6421 aatgcccgga ataaaggttt tatcgggcct gttgattccg gtcagcgtat ccaacgtggc
     6481 ggtcgaattg tcatcataat aattataccg gtttgtaatg ccggctaaaa aggaatgagt
     6541 attcccaaca accttatccc atatcagttg tgcaaaagca atcttctgat cacccatata
     6601 tggcgtagtg ccatagtatg aattttgctg atgtccggtt gcagagaaag atgctattat
     6661 tttttccgaa agtggcaatt gataatttcc gatcagttcc caacggctgg tgtatatact
     6721 ttctccatat acactgtcag tgcccctgaa actcctattc caattcatat ctccgcccca
     6781 ccggtcttca tagaaatatc ttcctgccag tgaagcacta cggttgtact ttcttgaaaa
     6841 atttgtttta ctaaaaacag aaaccctgtg ttgaagtgct atgtccgtaa actggtcatt
     6901 attcctgtca aacggatgtt ggtaattaaa ataatttact cccatcagag aattaatctt
     6961 tttcccagcc ttgaaacgaa ttccggcatc aaaattatgt tccagccagc ttgttgtcat
     7021 aatgtttgct gtaaaacggg gagccttatc cggcgacttt gtaataatat tgatcagccc
     7081 gccgattgcc tcagaaccat acaaccctga agctggacct ttgactatct ctactctttc
     7141 tattaattgt gttgggatac caaacaagcc gtaaaccgat gccaggctgc tgacaatggg
     7201 cataccgtca atggtgatca ttgtataagg cccttcaagg ccattgatat ggatatcgcc
     7261 ggtattgcaa acactgcagt tgagttgcgg acgaacacca tttacgttct gcaaagattc
     7321 aaatatactg ggcgacgggt ttttctttaa aaaatggggg gtgtacactt ctacagcaac
     7381 ggggctttcc gaccgcctga cagctttcat agttccggtc acgactattg catccagttc
     7441 tacctgaagc ggtgataccg gcaccagaat gaaatcattt tctccaactt ttactttctt
     7501 tctttcaaaa cctgtactgc taatctgcaa aactgcacct tcttttgtag caattgagaa
     7561 atttccattg atatcactta tggtataaac agatgaatcc tctgccatta ccga
//
LOCUS       DAORLS010000147         7479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5348_length_7479_cov_1.866245, whole genome shotgun sequence.
ACCESSION   DAORLS010000147 DAORLS010000000
VERSION     DAORLS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5348_length_7479_cov_1.866245"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>260)
                     /locus_tag="PK977_11600"
     CDS             complement(<1..>260)
                     /locus_tag="PK977_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRF18809.1"
                     /translation="ELGIPAEMIDRHPFPGPGLAIRILGEITEEKVQLLQEADHLYIK
                     ALKDNNLYATVWQAGAILLPVKSVGVMGDERTYEYTVALRAV"
     assembly_gap    261..295
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<296..1394)
                     /gene="guaA"
                     /locus_tag="PK977_11605"
     CDS             complement(<296..1394)
                     /gene="guaA"
                     /locus_tag="PK977_11605"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRF18810.1"
                     /translation="MAEKILILDFGSQYTQLIARAVREANVYCEIIPFHKSFEFEPGL
                     KGIILSGSPCSVNEENAPDVDIQDFISKVPVLGICYGAQLTAKRFGGLVAKSNKREYG
                     RAILQRKKEDVLVEGVSSQSQVWMSHSDTIKELPAGFELLADTESIPVAAFRKTNENL
                     YGVQFHPEVYHSKEGKRVINNFLVRICGCTQDWTPAHFITDTVETLKKQIGDRKVIMA
                     LSGGVDSTVAATLIHRAIGKNLFGIFVDNGVLRKDEFQNVLDTYAKIGLNVKGIDARE
                     HFYTKLAGKTDPEEKRKIIGSSFIDVFQEESKKIEGVELLGQGTIYPDVIESVSVHGP
                     SVTIKSHHNVGGLPDHLHLELVEPLRSLFKDE"
     gene            1577..2296
                     /locus_tag="PK977_11610"
     CDS             1577..2296
                     /locus_tag="PK977_11610"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRF18811.1"
                     /translation="MKRVLFFLVLIVLVFSSCRQILGKRIRGNGTVKTETRSIGEFKG
                     IEVNGAIDVYYKQDSVISVKVETDENLLEYVEVYVDNNTVVVEPKDGSNLRPSKSVKV
                     YISSPTVESLEASGASSIVTESKITVGELTVSLSGASDAEINAKSPKVNVDLSGASTA
                     ILSGETKDLKIECSGASKAKCYELLSENVTADLSGASDAQVFASVKIDAHVSGASDLK
                     YKGNATVSQETSGAASVKKAE"
     gene            2365..3729
                     /locus_tag="PK977_11615"
     CDS             2365..3729
                     /locus_tag="PK977_11615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF18812.1"
                     /translation="MYSRRKFIKDGSLAAGGLLLASSSFSNFFIPRAPKVVIIGAGFA
                     GLSAAYYLYKKKIDFVILEARNRIGGRVFSHIMDQKENLVIELGAEWVGNSHERLQNL
                     CNVFGLELQKNQFDSHLIYQGRYYKNGEWDYSEGWKKKFQSIIDSYSKLTHADKLKLD
                     KMDWWRFLVNNGCEGRDLDLRELLDSTDFGETIRSVSAFAALAEYAESSEKNEMDLKI
                     KGGNSMLAKKIAAAIGEEKIKLQHKVNRITQNAKTGVTVYCENGQVFKADKIICTAPT
                     FAVKNIKWEPGLPADQINAMNELQYARINKNPLLFKKRFWKDESFDMVTDQSPHYFYH
                     ATKNQNSKKGVLISYTIGEKAAVVANQTDDWRENMVMQTLKPHFNDAKEEFERMTNYY
                     WGNDMYSHGAYAVYGKGQWFRVMPILKRSHIHTHFAGEHLAEWQGFMEGAINTGEEAA
                     AKIR"
     gene            complement(3751..>4487)
                     /locus_tag="PK977_11620"
     CDS             complement(3751..>4487)
                     /locus_tag="PK977_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRF18813.1"
                     /translation="SIGYNKNYTVGVWCGNFSGAGSADLSGANTATPLLFKIFNTIDY
                     DSDEEWFTQPQDCDIRQICSETGLLPSAHCSSITTDYFIPLISSTKTCENRQEIMISP
                     DEKIAYCKSCAPASGYKKKWYKITEPDMQAWLEENRIAYEKIPQHNPECELIFKGAAP
                     VITSPVNGTEYLINKRDPEPLQLTCKTANDVIKVYWYINNKFFKSCNAGEKQFFVPEE
                     GPVKISCTDDKGRNRNIKILVKYVSL"
     assembly_gap    4488..4590
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4591..6140)
                     /gene="pbpC"
                     /locus_tag="PK977_11625"
     CDS             complement(<4591..6140)
                     /gene="pbpC"
                     /locus_tag="PK977_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRF18814.1"
                     /translation="MKKRLHLQFPWFPKKEQVPARKYFTRRGILRFLKRTGIGIISLI
                     LLFFILNWIFPLPDKVEYSTIITDDKGEVINAFLTNDQKWRMKTELEEISPLLQKTIV
                     AKEDRHFYSHPGVNPFAVIRAFFSNIFHMRRMSGASTITMQVAKMLEPGKRNIWSKTR
                     EMFRALQLELKYSKKEILQLYLNLVPYGGNIEGVKAASLLYFKKNPDHLSLAEITALS
                     IIPNKPGVMVPGRNNDFIVKERNRWLERFADRKVFSQKEIEDALAEPLTATRGTVPHY
                     IPHLSYKLKKTGSDIIKTNIILNTQLKTEKLVEDYIRIQRLKNIRNAAVVIIDNKTHK
                     VITYVGSSGFYDTTDGGQVNGAAAVRQPGSTLKPLLYGMCFDEGLLTPKTVMTDVAVN
                     FDGYAPENYDDKFNGYVSVEYSLEHSLNIPAVKGLRFLGHEKMIQKLSNCNFRQVQKD
                     RRKLGLSMILGGCGTTLEELTALFSSFANNGIYVKPTFIQTDTVQQEVIVISPAATFM
                     INEILSKV"
     gene            6484..>7479
                     /locus_tag="PK977_11630"
     CDS             6484..>7479
                     /locus_tag="PK977_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18815.1"
                     /translation="MRIKFLIVPLIAAGAIFLSSCKKNSVSLSFTNAKNEVPELGNLV
                     FRFSHSLATDSMLNVWDSTEYISFEPAIPGRFRWQSPDELVFSPSQPLNPATNYKAKI
                     KSAVLRFSKYNSVKNGDKISFHTPELTLNNYQVLWVGESSTSAIPQIDLFFNYRINPE
                     ILKDRLKLEVEGKKADFNMITISPDNKISVRINGLKSEDKDSEVKVVIEKGLKPEKGN
                     MSTSESITSTMTIPSPYVLTVQNLESEYDGTEGVVRITTSQQLTGEGIKSYLKFDPEV
                     EFAVEPNDFGFTLRSDKFDAEKSYALVINKGLRGKLGGVMKEEYNGSVAFGELEAN"
BASE COUNT         2115 a         1470 c         1542 g         2214 t
ORIGIN      
        1 taacagcccg taaagccact gtgtactcgt atgttctttc gtctcccata acaccaacac
       61 tttttactgg cagtaagata gcacctgcct gccaaaccgt tgcatataaa ttgttatcct
      121 taagggcttt tatatacagg tgatctgctt cctgcaacaa ctgtaccttt tcctctgtaa
      181 tctctcctaa aatacgaatg gccaggcccg gcccggggaa gggatgacgg tcgatcattt
      241 cagcaggaat tcccaattct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncttca
      301 tctttgaaga gagaacgtaa tggttctacc agttcaagat gcaaatgatc cggaagacca
      361 cccacattat ggtgtgattt gatggttacc gaagggccgt gtactgacac actttctatt
      421 acatccgggt aaatcgttcc ctggcccaat aactcaacac cctcaatttt tttagattct
      481 tcctggaata catcaatgaa agagcttcct ataatctttc ttttttcttc agggtctgtt
      541 ttgccagcta atttggtata aaaatgttcc cgggcatcaa tacctttaac attcaatcca
      601 atttttgcat aggtatccag tacgttttga aactcgtctt ttcgcagtac tccattatct
      661 acaaaaattc caaagagatt tttgccgatt gccctatgta tcaatgttgc tgcaacagtt
      721 gagtcaacac cgccacttaa tgccatgatc actttccggt ctccgatctg tttttttaat
      781 gtttcaacag tatcagtgat gaaatgagcc ggggtccagt cctgtgtaca gccacagatt
      841 ctcaccagga aattattaat gaccctttta ccttctttgg agtgatagac ttctggatga
      901 aattgtactc cataaagatt ttcattggtt tttctgaatg ccgcaacagg tatactttcc
      961 gtgtcggcca ataattcaaa acctgccggt aattctttga tggtgtcgct atggctcatc
     1021 catacctggc tttgtgatga aacaccttcc actaatacat cttctttttt tcgttgcaat
     1081 atggcccggc catattctct tttattgctt tttgccacca gcccgccaaa gcgtttcgct
     1141 gtcagctgcg caccatagca aatacccagt accggtactt tgctgataaa gtcctgtata
     1201 tcaacatccg gtgcattttc ctcatttaca gaacagggcg aaccggataa aataatgcct
     1261 ttgaggccgg gttcaaattc aaaagattta tgaaaaggaa tgatctcgca ataaacatta
     1321 gcctctctga ctgcacgggc tatcaattga gtatattggg aaccaaaatc gagaatgagt
     1381 attttttctg ccatattcct gcgaaggtaa aaaaagccgg ttagtttact gataattggc
     1441 taaaaggcaa gcctggtttg aattttagga tcattcagat tgttccctat tcctgtaacc
     1501 cgaaatgaaa gaatagcgtc aaagctggta aatcatcgat ttttgaactt aattttaaat
     1561 agtaaaaatt taccacatga aacgggtatt gtttttttta gtactgatag ttttggtttt
     1621 tagttcctgc cgccagattt tgggcaaaag aatccgcgga aatggtacgg taaaaacaga
     1681 aacaaggagt attggcgaat tcaagggaat agaagtaaat ggcgctatag atgtttatta
     1741 taaacaagat tctgttatct ctgtaaaagt ggaaacagat gagaacctcc tggagtatgt
     1801 tgaagtgtac gttgataata atactgtagt ggtagaacct aaggatggat caaacttaag
     1861 gccatcaaaa tcggtaaaag tatatattag cagccccaca gttgaatcat tagaagccag
     1921 tggtgccagc agcatcgtta cagaaagcaa aataaccgtg ggcgaactta ctgtaagtct
     1981 cagtggtgcc agtgatgcgg aaataaatgc aaaatcaccc aaggtgaatg ttgatctttc
     2041 cggtgcaagt actgcaatcc tttcgggtga aacaaaggat ttaaaaatag aatgcagtgg
     2101 tgcatcaaaa gcaaagtgct atgaactgct ctctgaaaat gtcactgctg atctatccgg
     2161 ggcaagtgat gcacaggttt ttgccagtgt aaaaattgat gcccatgtat caggtgcatc
     2221 tgatctgaag tataaaggaa atgcaacggt cagccaggag acaagcggag cagcaagtgt
     2281 aaaaaaggcc gaataactaa cctctttgtg gtattttttt ctacgggaaa ttcctgaatt
     2341 tcacaggaac caaaaaccaa ctatatgtac tcccgtagaa aatttattaa agacggctct
     2401 ctggcagccg gtggattatt attggcttct tcttctttca gtaatttctt tattccaagg
     2461 gcacccaaag ttgtaataat tggtgcaggg tttgccggtt tatctgccgc ttattatttg
     2521 tataaaaaga aaatagattt tgtgatactg gaggcccgca accgtattgg tggcagggtt
     2581 ttttcccata taatggatca aaaggaaaac ctggtgatag aattaggcgc tgaatgggtg
     2641 ggtaattctc atgaaagact gcaaaacctc tgtaatgtgt ttggcctaga acttcagaaa
     2701 aaccagtttg attcccacct gatttaccag ggcaggtatt acaaaaacgg tgaatgggat
     2761 tatagtgaag gctggaagaa aaaatttcag tcaattattg atagctattc taaactgaca
     2821 catgcggata agctgaagct agataaaatg gattggtggc gcttcctggt gaataatggt
     2881 tgcgaaggca gagatcttga tctcagggaa ttgcttgaca gtaccgattt tggggaaact
     2941 attcgcagtg tttccgcatt tgctgctctg gcagagtatg cggaaagcag tgagaaaaat
     3001 gaaatggatt tgaaaattaa aggtggcaac agtatgctgg caaaaaagat agcagcagca
     3061 attggagagg agaaaataaa acttcagcat aaagtaaacc gtatcacaca gaatgcaaaa
     3121 actggtgtta cggtttattg cgagaacggc caggtattta aagccgacaa gatcatttgt
     3181 acagcgccga cttttgcggt gaaaaatatt aagtgggagc caggcttgcc ggcagaccag
     3241 ataaacgcca tgaatgaatt acaatatgcc cgcatcaata aaaatcctct attgttcaaa
     3301 aaacggtttt ggaaagatga gagttttgat atggtgactg atcagtcacc ccattatttt
     3361 tatcatgcta ccaagaatca aaattcgaag aagggggtac ttatctctta tacaattggt
     3421 gaaaaagcgg ctgtggtggc caaccagacc gatgactggc gggaaaacat ggtgatgcaa
     3481 accctcaagc cgcattttaa tgatgctaaa gaagaatttg agcgaatgac caattattac
     3541 tggggcaatg atatgtattc gcatggtgct tatgcggtgt atggcaaagg tcaatggttc
     3601 cgagtgatgc ctatattaaa gcgttcccat attcataccc attttgcagg tgaacacctg
     3661 gcagaatggc aaggttttat ggaaggtgca atcaacaccg gggaagaagc agccgcaaag
     3721 ataagataag ctaaacagca gttgcgggaa ttaaagggaa acgtatttaa caagtatttt
     3781 aatattcctg tttcttcctt tatcatcagt gcaggaaatt tttactggtc cttcttccgg
     3841 tacaaagaat tgtttctcgc cggcattaca gcttttaaag aatttattgt tgatgtacca
     3901 gtacaccttg attacatcat tggcagtttt acatgtcagc tgcagtggtt cagggtctct
     3961 tttattgata aggtattcag ttccgtttac aggcgaagtt ataaccggag ctgcgccttt
     4021 gaatattagt tcacactcag gattgtgttg tgggatcttt tcataagcaa ttctgttttc
     4081 ttcaagccat gcctgcatat caggctctgt tattttatac catttctttt tatagccact
     4141 tgccggtgca caacttttgc aataagctat tttctcatcc gggctgatca ttatttcctg
     4201 cctgttttca caggttttgg tagaggatat caagggaatg aaataatcag tagtaatact
     4261 gctgcaatgg gcagatggta gtagacctgt ttcgctgcat atttgcctga tgtcgcagtc
     4321 ctggggttgt gtaaaccatt cttcatcgct gtcgtaatca atagtgttga agattttaaa
     4381 aagaagcggt gtggcggtat tggcaccgct aagatctgct gatcctgcac cagaaaaatt
     4441 tccacaccaa acacctactg tgtagttttt attgtagcca atgctccnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttactttcg acaagatttc atttatcatg
     4621 aatgtggcag caggacttat tacgatcact tcttgctgta ctgtgtcagt ttgaatgaaa
     4681 gtaggcttga cataaattcc gttgttagca aacgaagaaa acaaggcagt aagttcttcg
     4741 agggtggtac cacagccgcc tagaatcatt gaaagaccca gctttcgcct gtctttttga
     4801 acctgcctga aattgcagtt ggataatttc tgaatcattt tttcatgacc tagaaagcga
     4861 agacctttta cagcaggaat gttcaatgaa tgttccagcg agtattcaac tgatacataa
     4921 ccattgaatt tatcatcata attttcaggg gcataaccat caaagttgac tgccacatct
     4981 gtcatcactg tttttggtgt aagcaaacct tcatcgaagc acatgccata aagcagtggt
     5041 ttcagcgtac tccctggctg ccggactgca gcggcaccat ttacctgtcc tccgtcagta
     5101 gtatcataaa aacctgatga acccacatag gtaattactt tatgcgtttt attatcaatg
     5161 atcactacgg cagcgtttct gatatttttc aaccgctgga tgcggatata atcctccacc
     5221 agtttttctg ttttcaattg tgtattcaga atgatattcg ttttgattat atcagatcct
     5281 gtctttttga gtttataaga aagatgcggg atgtaatgag gcactgttcc tctggtggcc
     5341 gttaatggtt cagccagggc atcttctatt tctttttggg aaaatacttt tctgtccgca
     5401 aatctttcga gccagcggtt tctttctttt acaatgaagt cattgtttct gcccggcacc
     5461 atcacccccg gtttgttggg gataatgctc agcgctgtta tttcagccag tgaaagatga
     5521 tccgggtttt tcttgaagta aagcagcgat gctgctttta ccccttcaat attaccacca
     5581 taaggaacga ggttcaggta tagttgtaaa atttctttct tactgtattt aagttccagt
     5641 tgtaatgccc tgaacatctc ccttgtcttg ctccagatat ttcttttgcc cggttccagc
     5701 attttggcta cctgcatggt aatggtgctg gcgccggaca tcctccgcat atgaaagata
     5761 ttgctgaaaa aagccctgat aacagcgaac ggatttacac cgggatgaga atagaaatgc
     5821 ctgtcttctt tagctacaat tgtcttttgc aacaaagggg aaatctcctc cagctctgtt
     5881 ttcatacgcc atttttgatc gttggtcagg aaggcattaa ttacttcacc tttatcatcg
     5941 gttattatgg tagaatattc cactttgtcg ggtaagggaa atatccagtt caggataaaa
     6001 aagaggagga tcaaacttat aatccctatg ccagttcttt tcagaaaacg aagtatgccc
     6061 cggcgggtaa aatactttcg ggccggcacc tgttcttttt ttgggaacca gggaaattgc
     6121 aaatggaggc gtttcttcat cagataggga tgcttgatgg ctccgttgcc acttaacaaa
     6181 tatttatctt ctttaaactt gacattctga attgctgtgc tttaccttta ctatgcaatt
     6241 attcacatcc attatggatt cttcagaccc atcctcttcc tcacgggcag ataggcgttc
     6301 cacccgggat attccccctt agctttccat acgctaattt cagttatttc cgtcattacg
     6361 gctaagccaa ttgtgcataa caaagcaata gcttaatttt ttaactaatc aatcttttct
     6421 aacttctcac tcataaccac actgccggcc ctgacagccg gctagcctta aacctacatc
     6481 tttatgcgta tcaagtttct tatagttccg ctaatagcag ccggagctat ttttttatct
     6541 tcctgtaaga agaattccgt cagcctcagt tttaccaatg caaaaaatga agtacctgaa
     6601 ctgggaaatc tagttttccg tttcagtcat tccctggcca ctgattccat gctgaatgta
     6661 tgggattcta ccgagtacat ttcatttgaa ccggctatac ccggccgctt tcgctggcaa
     6721 agcccggatg aattggtttt ctctccctca cagccgctta acccggccac taattacaag
     6781 gcaaagatca aaagtgctgt gcttcgtttc agtaaatata attctgtaaa gaacggagat
     6841 aagatcagtt ttcacacacc agaacttacg ttaaacaatt accaggtgtt atgggtggga
     6901 gaaagcagta catcagctat tccacagatt gatctgtttt tcaactacag gataaatccg
     6961 gaaatactaa aagataggtt gaagttagaa gtagaaggaa agaaagcaga ttttaatatg
     7021 atcaccatct caccggacaa taagatatct gtccgcatca acggattgaa atcagaggat
     7081 aaagacagtg aggttaaagt ggtaattgaa aaaggactga agcccgaaaa agggaatatg
     7141 tccacttcag aatcgattac cagcacaatg acaattccct cgccttatgt gttgacagtt
     7201 caaaatcttg aatctgagta tgatggtaca gaaggggtag taagaattac taccagtcag
     7261 caactcacag gagaggggat caaatcctat ttgaaatttg atcctgaagt tgagtttgca
     7321 gttgaaccca atgactttgg atttacttta cgaagcgaca aatttgacgc agaaaaaagc
     7381 tatgctttgg ttattaacaa gggtcttcgt ggtaagctgg gtggtgttat gaaagaagag
     7441 tataatggga gtgttgcttt tggcgaatta gaggctaat
//
LOCUS       DAORLS010000148         7464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5362_length_7464_cov_2.087596, whole genome shotgun sequence.
ACCESSION   DAORLS010000148 DAORLS010000000
VERSION     DAORLS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5362_length_7464_cov_2.087596"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..84)
                     /locus_tag="PK977_11635"
     tRNA            complement(11..84)
                     /locus_tag="PK977_11635"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Asn,seq:gtt)
     gene            complement(161..520)
                     /locus_tag="PK977_11640"
     CDS             complement(161..520)
                     /locus_tag="PK977_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRF18816.1"
                     /translation="MKKSLTWHKIADHINELGFAENNIALAWLNEKKICIGRYKDQVF
                     AFAYKCPHAGGLMTDGFIDALGNVVCPLHRYKFDMRNGRNVSGEGYYLKNWPVEIREE
                     GVFVGVEETGGFWNIFR"
     gene            complement(522..1712)
                     /gene="hflX"
                     /locus_tag="PK977_11645"
     CDS             complement(522..1712)
                     /gene="hflX"
                     /locus_tag="PK977_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRF18817.1"
                     /translation="MLEKKNIIQQEERAVLVGLVHKDQTEQQVHEYLDELAFLAETAG
                     AITVKRFTQKLPHPDSRTFIGKGKLEEIRQYIQGKNIRIVIFDDELSGAQINNIEKAL
                     EVKTIDRSDLILDIFARRAKTAQAKAQVELAQYQYILPRLRGMWKHLERLGGGIGTRG
                     PGESEIETDRRIVRDKISLLRKRLAEIDKQAFTQRKDRGELIRVALVGYTNVGKSTIM
                     NLLSKRDVFAENKLFATLDTTTSKVVFENTPFLLSDTVGFIRKLPHHLVESFKSTLDE
                     VREADILLHVVDISHPAYEDQLGVVNKTLQELDAFDKPTLTIFNKMDLYEKNTFDEWL
                     EESTKKEILEDLYERWQRETNGNAVFVAAIEKRNLDMLRKTILDKVRELYRIRYPYKT
                     EFIY"
     gene            complement(1823..2542)
                     /locus_tag="PK977_11650"
     CDS             complement(1823..2542)
                     /locus_tag="PK977_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF18818.1"
                     /translation="MKKFLAAAFCALFSFTLVAQEKPAPKKKVIDMSNRANDHFLLQF
                     GYTSWAGKPDSINTKGLSKSINAYFMFDFPFKTNPKLSMAFGPGIATDHILFTKTYVG
                     IKDQTNSIYFTDQADTNHFKKTKLATAYLEAPIELRFSANPETGKGFKAAIGVKIGTL
                     LNAHTRNTKFEDKNGNALNDYVMKESSKRFLNKNRISAQARFGYGHFTLYGSYQLTTL
                     FRDGAGPEVRPFSIGLTLSGL"
     gene            2651..2724
                     /locus_tag="PK977_11655"
     tRNA            2651..2724
                     /locus_tag="PK977_11655"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Ile,seq:cat)
     gene            2906..3496
                     /locus_tag="PK977_11660"
     CDS             2906..3496
                     /locus_tag="PK977_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRF18819.1"
                     /translation="MTENEQIKRLLTLIAVNNDQAAYKELFVLLYYRLKQFAFTILKS
                     HEEAEELVSDVFIKIWEKRSQLTTVESPRLYLYTTTKNLAITRINKQKKQISMAPDDW
                     LVQLNSIYFDPEKLMITEEVFRQIRQAVNDLPPRCRLIFKLIKEDGLRYKEVADLLHL
                     SVKTVEAQMAIALRRVGNCMQMNIKAPVTPVSPKKS"
     gene            3561..4664
                     /locus_tag="PK977_11665"
     CDS             3561..4664
                     /locus_tag="PK977_11665"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRF18820.1"
                     /translation="MSQEKFWILLSKKLANEATLPDLKELELLIQQHPEWQHAVQTIG
                     DVWTSRSANDFMEAEDAFMLHQQRMREMNIDLGDGIVEVEKGKTKRIRWYWAAAAAAI
                     IGGFFLFNSMTGLRGGEESGRGIAREVNEISTRPGSKSKIELPDGSIVWLNAGSKLTY
                     DKNFGKDLREVTLTGEGYFDVMKMIEKPFIIHTSSINIKVLGTVFNVKAYPEDKHTET
                     SLIRGSVEVTIKNRPNDKIILSPSEKLVVENDAIVKKGKVAESKYSTSAAAIKTLMSV
                     NKLKYSPADSTVAETEWIKNRLVFRDESLAELAVRMERWYDVSIDITDEGLQQKRVTG
                     IFENETIDQALGALKIFIPFKYEQTGNKIIIHR"
     gene            4806..>7464
                     /locus_tag="PK977_11670"
     CDS             4806..>7464
                     /locus_tag="PK977_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF18821.1"
                     /translation="MKKTETAAVSQKALLFLKIARIMKLTIALLLFACLQVSAKGLGQ
                     ERITLKMNEAEIKKVLFAIEKKSDYRFLFSEESVKGKPKVNVDVTNATLNEVLDKILV
                     NTGIAYKVLGTNLVVLKEGITSAEMISPEIRITGKVTSATGEPLAGVSVSVKGSRAGT
                     TTDANGNFSITVLDDAVLVFSSVGYESTEVSVKGKTSLTVVLKVSEKVQDAVVVIGYG
                     TAAKRDLTGSIVKVKGDEIAAQPNANPLASLQSKVAGLQIVNTAIPGSAPDVRIRGTI
                     SVGSIRPVYIIDGIFSDNMDFVNPSEIESVEVLKDPSSLAIFGIKGAAGAILVTTKKA
                     KTGQVNINFNTSYGTKKLVDKIQLANGAEFRQLLEFEANNRVADDPSNNSLLNFVNNV
                     GTPGLGAYTGNTDWIDAVTRTAKFSTTNLSVDGSTEKNRFHFGLGYTYDEGLVKHVKY
                     DRLTINVNDEFRVNKKWKLGFGLIGSKEKLPYNSGALENARRALPIIPSETKQFYTKN
                     PYGVDSGYYNLYATTPIIQNSETNPLATLENNWDNRIDDRYRFVANAFVDINITKDLN
                     FRSTWYGDLSFRNAREYTSLYDMYDPTKAAGSEVFPFRSLTAVRQDLINTKAFQQDYI
                     ATYKKKFGDHSITATAGLTTYYFNFEHVAGTVSQRTPGVNIIPDNKRFWYLNTGFGDI
                     KSVIPTGQSEYATASGLARVLYNYKNKYFFNASFRRDGSSQINTDYSKKWQNFWALGA
                     AWEITKESFMDNQKIFNYLKLKASTGVLGNFTAQGKAYPAYPTVNPTASAVFGENLVP
                     VLEPDYLFDPNLKWETVNSTEVGIEAEMLKGKLRFEANYYYKKTDNLIVLLKGSGSLQ
                     TLTNNGQIENRGFELSANWNHKINEDLSLS"
BASE COUNT         2183 a         1482 c         1651 g         2148 t
ORIGIN      
        1 ttcttcttcg ctccccctgt tggacttgaa ccaacgaccc tctgattaac agtcagatgc
       61 tctaaccaac tgagctaagg aggaatacct ttccgcttgt tttgcgggca gcaaaaatag
      121 ggattttaag tttttccaca aggtaattca gcatcaattt ttaacggaaa atattccaga
      181 atccccctgt ttcttccacg cctacaaaga caccttcttc cctgatctct actggccagt
      241 ttttcaggta atagccttca ccgcttacat tacggccgtt ccgcatatcg aatttgtacc
      301 ggtgcagggg gcaaaccaca ttgcccagtg catcaataaa gccatcggtc attaaacccc
      361 cggcatgcgg acatttatag gcaaaggcaa aaacctgatc tttataccgg ccgatgcaaa
      421 tctttttttc attcagccag gcaagggcga tattgttttc cgcaaacccg agttcattga
      481 tatggtctgc aatcttgtgc caggtgaggg atttcttcat atcaatatat gaactcggtt
      541 ttgtatggat aacggatacg atagagttct cttactttgt cgaggatcgt tttgcggagc
      601 atatccagat tgcgtttttc aatagctgct acaaagactg catttccgtt ggtttctctt
      661 tgccatctct catagaggtc ttccaaaatt tctttttttg tgctttcttc cagccactcg
      721 tcaaatgtat ttttttcgta gaggtccatt ttattgaaaa tggtgagggt tggtttatca
      781 aaagcatcta actcctgcaa tgttttatta actaccccca gctgatcttc gtaggcagga
      841 tgcgagatgt ctaccacatg caatagtata tcagcctctc ttacttcgtc cagggtgctt
      901 ttgaaacttt ctaccaggtg atggggtagt ttgcgtataa aacctactgt atcactgagc
      961 aggaatgggg tattttcaaa aaccacttta ctggtagtgg tatccaatgt tgcaaacaat
     1021 ttattttctg caaagacatc tcttttactc agcaggttca tgatggttga cttgcctaca
     1081 ttggtatatc ccactaaagc cactcgtatt aactcacccc tgtctttgcg ctgggtaaag
     1141 gcttgcttat ctatttctgc cagtcgctta cgtagcaatg atattttgtc tctcacaata
     1201 cgacggtcgg tttctatctc actttcacca gggcctcttg ttccgatacc gccacctagt
     1261 ctttcaaggt gtttccacat acctctcagc cggggtaaga tatattggta ttgcgccagt
     1321 tcaacctgtg cttttgcttg tgctgttttg gctctgcggg caaaaatgtc gagaattaaa
     1381 tcgctcctgt ctatcgtttt tacttccagg gctttttcaa tgttgttgat ctgtgcaccg
     1441 gaaagctcat catcaaaaat gacaatccgg atatttttcc cctgaatata ctggcggatt
     1501 tcttccagtt ttccctttcc aataaaagta cggctgtcgg gatggggtaa tttttgggta
     1561 aaccgtttta cggtaatggc acctgctgtt tcagccagga aagctaattc atcaaggtat
     1621 tcatgcacct gctgctctgt ctggtctttg tgtacgagac ccaccagtac cgctctttct
     1681 tcctgttgaa tgatattttt cttttcgagc atatagaata aaagccccgc agcagcggag
     1741 caggtgcaaa gataaacagg gttatttact taaaaggaaa agggctttac cagttggcaa
     1801 agcccttttt caaaaatctt atttatagtc cgctaagggt aagtccgatt gagaagggcc
     1861 ttacttcggg gccggcacca tcccggaaga gtgtagtaag ctgatagctt ccgtaaagtg
     1921 tgaaatggcc atagccaaat cttgcctgtg cggaaatgcg gtttttgttc aggaatcttt
     1981 tgctggattc cttcattaca tagtcattta aagcatttcc gtttttgtct tcaaactttg
     2041 tgttgcgggt atgggcattt aataatgtac ctattttaac gccaattgca gctttgaatc
     2101 ctttgcctgt ttccgggttg gctgaaaagc gcaactcgat tggagcttcc aggtaggccg
     2161 ttgccagttt tgttttctta aaatgattgg tatcagcctg atcagtgaaa taaatggaat
     2221 ttgtctggtc tttgatacca acgtatgttt tcgtgaaaag aatatgatcg gttgctattc
     2281 cggggccaaa agccatgctc agcttagggt ttgttttgaa agggaagtca aacatgaagt
     2341 aagcattaat acttttggat aatcctttgg tattgatact gtctggtttt ccggcccagc
     2401 tggtgtagcc aaactgaagc aggaaatgat cattggcacg gttgctcatg tctataacct
     2461 tctttttagg ggcaggtttt tcctgggcaa ctagagtgaa actgaatagg gcacagaaag
     2521 cagcagcgag aaatttcttc ataagagtta ttaaggtcgc aaatatattt ttctttcggt
     2581 taaagattgg ttaagaagga gtaataatta ttatttgtta cttttgcagc tctttgagca
     2641 aaaatgctca ggacccttag ctcagacggt tagagcatct gactcataat cagagggtcg
     2701 ctggttcgat cccagcaggg tccacccagc cattgtgatt gtttcgcaat ggctttttta
     2761 ttactatcca tcggggtaat tacgaggaat aaatgtgttg tgcaaaatca ataaataata
     2821 taatttctcc ctgaaaaaat atttctgttt ggaagtcact ctttttccaa tttcaattcc
     2881 ttagattgag ccatcttttg ctcatatgac tgaaaacgaa caaataaagc gattgcttac
     2941 gctaattgca gtaaacaacg accaggccgc atacaaagag ttatttgtac tgctttacta
     3001 ccggctgaag caatttgcat tcactattct gaaatctcat gaagaggcag aagagctggt
     3061 gtctgatgtt ttcataaaaa tatgggagaa aagaagccag ttgaccaccg ttgaatctcc
     3121 ccgcttatat ttatatacca ctaccaagaa cctggcgatt acccggatca acaagcaaaa
     3181 aaagcagatc agcatggcgc cggacgactg gctggtgcag ctgaacagta tttatttcga
     3241 tcccgaaaag cttatgatca ccgaagaggt ttttcgtcaa atccggcagg ccgtaaatga
     3301 ccttcctccc cgttgtaggt tgattttcaa gcttataaaa gaagatggat tgcgttataa
     3361 ggaggtggcc gatttactgc acctttctgt aaaaactgtt gaggcacaaa tggccattgc
     3421 tcttcgaagg gtcggtaatt gtatgcagat gaatataaaa gcccctgtta cccccgtttc
     3481 tccaaaaaaa agttaaaatt cttttagggg tattgcctgt tttttagtgt cttaatggtt
     3541 tattctaatc aactcgttga atgagccagg agaaattttg gattttactg tcgaaaaaat
     3601 tagcgaacga agccaccctg ccggatctga aggaactgga actgctcatt cagcaacacc
     3661 cggaatggca gcatgcggta cagactattg gggatgtttg gacaagcaga tctgccaatg
     3721 attttatgga agctgaagat gcttttatgc ttcatcagca gcggatgagg gaaatgaata
     3781 tcgatctggg agatggtatt gtggaggttg agaaaggaaa aacaaaaagg atcaggtggt
     3841 attgggcggc tgcagcagct gccatcattg gaggtttttt cctgttcaat agcatgacag
     3901 gactgcgagg cggtgaagaa agcggacggg gtatagcgag agaagtaaac gaaatcagca
     3961 caaggccagg ctccaaatca aaaattgaat taccggacgg atcaattgta tggctgaatg
     4021 ccggaagcaa gcttacctat gataaaaatt tcggaaagga cctgcgggaa gtgacactaa
     4081 caggagaagg ttatttcgat gtgatgaaga tgatagaaaa gcccttcatt attcatacat
     4141 caagcatcaa tataaaagta ttgggtactg tattcaatgt aaaggcttac ccggaagata
     4201 aacatacgga gaccagcctg ataagaggaa gtgtggaggt gacaatcaaa aacaggccga
     4261 atgacaagat cattctttcc cccagcgaaa aactggtagt agagaatgat gcgattgtta
     4321 aaaagggaaa agtagcggag agtaagtaca gcacttcggc tgctgctatc aaaacactga
     4381 tgtcggtaaa taaactgaag tatagcccgg cagacagtac ggtagcagaa acagaatgga
     4441 tcaaaaacag gcttgtgttc agggatgaat cattggcaga actggcagtg agaatggaaa
     4501 ggtggtacga tgtaagtatt gatattacag atgagggcct gcagcaaaaa agagtgacag
     4561 gaatttttga aaacgaaacg attgaccagg cactgggagc tttaaaaatt tttataccat
     4621 ttaaatacga acaaaccgga aataaaataa ttattcaccg ctaatgctta aggccatatg
     4681 attgctgaca acacatcctg agcgagggaa taaaaaagcg gaaccccgct gcaacggaat
     4741 tccgccgaaa tgattgcaaa cgagacactt aagtgtctgc attcttaacc ctccaactgt
     4801 aaattatgaa aaaaactgaa acagctgctg tttcgcaaaa ggctctgtta tttttaaaga
     4861 ttgccaggat tatgaaatta acgattgcac tattgctttt tgcctgtcta caggtatctg
     4921 caaagggtct tggacaagag agaattaccc ttaagatgaa tgaggcagag atcaagaaag
     4981 tgctttttgc gattgagaaa aaaagtgatt accgctttct cttttctgaa gaatcagtaa
     5041 aaggcaaacc caaagtaaat gtagatgtaa ccaatgctac cctcaacgag gtgcttgata
     5101 aaatactcgt taacaccggt attgcttaca aagtacttgg cactaacctg gttgtactta
     5161 aagaaggaat tacttcagcg gaaatgattt ctcctgagat caggattacc ggtaaagtta
     5221 cttctgctac cggtgagcct ttggcaggtg tatccgtatc tgtaaaagga tcaagggcgg
     5281 gtacgacaac tgatgccaat ggtaactttt ctattaccgt tctggatgat gctgttcttg
     5341 tattcagctc tgttggatac gaaagcactg aggtttctgt aaaaggaaaa acatctttga
     5401 ctgttgtact gaaagtttct gaaaaagttc aggatgccgt ggtggtaatt ggttatggta
     5461 ctgctgccaa aagagacctt accggttcaa tcgtaaaagt aaaaggcgat gagatcgctg
     5521 ctcagcccaa cgctaaccca ttggcttctt tacaatcaaa agtggctggt ctgcagattg
     5581 tgaatactgc cattccgggt tcagctccgg atgtaaggat caggggtact atcagtgtgg
     5641 gttccatcag gcctgtttat atcattgacg gtattttcag tgataacatg gattttgtaa
     5701 accccagcga gattgaaagt gtggaagtat taaaagatcc ttcttcactg gccatctttg
     5761 gtatcaaagg tgctgccggt gctattttgg taacaaccaa gaaagccaag actggccagg
     5821 ttaacattaa cttcaacaca agctatggta ctaaaaaact ggttgataaa attcaactgg
     5881 ccaatggtgc agaattccgc cagttattgg agtttgaagc aaataacagg gtagctgatg
     5941 atccttccaa taattcattg ctcaactttg taaataatgt aggaacaccg ggtctgggtg
     6001 cttataccgg caatacagac tggattgatg cggtaacccg tactgccaag ttcagcacta
     6061 ctaacctgag tgtagacgga agcacagaaa agaaccgctt ccactttggt ttaggatata
     6121 cttatgatga aggccttgtc aagcatgtaa agtatgatcg tttgaccatt aacgtaaatg
     6181 atgaattcag ggtaaataag aaatggaaac tgggctttgg cctgattggt tctaaagaaa
     6241 agcttcccta taatagtggt gccctggaaa atgcaagaag ggcattaccc atcattcctt
     6301 ctgaaacaaa acagttctat actaaaaacc cttatggtgt tgattccggg tattataatt
     6361 tatatgcgac tactcctatt atccagaatt ctgaaacaaa tccattggca acattggaaa
     6421 acaactggga caacaggata gatgaccgtt accgatttgt agccaatgca tttgtggata
     6481 tcaatattac caaagacctg aacttcagaa gtacctggta cggagatctg agtttccgta
     6541 atgcaagaga atatacctct ttgtatgata tgtatgatcc taccaaggca gctggttcag
     6601 aggtattccc cttcaggagc cttactgccg taagacagga cctgatcaat acaaaggctt
     6661 tccagcaaga ttatattgca acgtataaga agaaatttgg cgatcactcc atcactgcta
     6721 ctgcaggttt aacaacttac tacttcaact tcgagcatgt agccggcact gttagccagc
     6781 gtacacctgg tgtaaacatt ataccggata ataagcgttt ctggtatctt aacacaggtt
     6841 tcggtgatat caagagtgtg atacctaccg gccaaagtga atatgctaca gcatcaggtt
     6901 tggcaagggt tctatataat tataagaaca agtatttctt taatgccagc ttcagaagag
     6961 atggttccag ccagatcaat acggactatt ctaagaaatg gcaaaatttc tgggcactgg
     7021 gtgctgcctg ggaaatcacc aaggaaagct ttatggacaa ccagaagatc ttcaattacc
     7081 tgaaattgaa agcttcaacc ggtgtgcttg gaaactttac tgctcaaggc aaggcttatc
     7141 cggcttatcc aactgttaac ccaacagcct ccgctgtatt tggtgaaaac ctggtaccgg
     7201 tattggagcc agattacctg tttgacccta acctgaaatg ggaaactgtt aattcaactg
     7261 aggtaggtat cgaagcagaa atgctgaaag gaaaacttcg tttcgaagcc aattattact
     7321 ataagaagac ggataacctg atcgtattgc tgaaaggttc tggttcatta cagacactga
     7381 caaataatgg acagattgag aacagaggat ttgaattatc tgctaactgg aatcataaaa
     7441 taaatgaaga tctcagtctt tctg
//
LOCUS       DAORLS010000149         7418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5398_length_7418_cov_1.546109, whole genome shotgun sequence.
ACCESSION   DAORLS010000149 DAORLS010000000
VERSION     DAORLS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5398_length_7418_cov_1.546109"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK977_11675"
     CDS             <1..181
                     /locus_tag="PK977_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TilS substrate C-terminal domain-containing
                     protein"
                     /protein_id="HRF18822.1"
                     /translation="KKKKLSRFFIDQKLSKIEKENTWVLESNKRIIWVVGFRIDNRFK
                     ITDQTKQVLKVTLEV"
     gene            complement(170..823)
                     /locus_tag="PK977_11680"
     CDS             complement(170..823)
                     /locus_tag="PK977_11680"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRF18823.1"
                     /translation="MELSITLIIIIITGLISFSAFSNHKIIDDLIFYPPSVTERGQWY
                     RFFTCGLIHADIGHLLFNMISLYMFGEFVERSFSTPILFGEKGKFLYLGMYVLALFVC
                     LVPTFIKHKNDYYYRSLGASGAVSAVVFAGILFDPTNKIGFFFIPPVIPGYIFGPLYL
                     IASTYLEKQAKDNVNHSAHIWGALFGIGFIIAAAAILKSDYQPAEQFWLKIKYSLNL"
     gene            complement(867..>1115)
                     /locus_tag="PK977_11685"
     CDS             complement(867..>1115)
                     /locus_tag="PK977_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRF18824.1"
                     /translation="RGKQLSSEGLATFIQARANESTKRIVFLIGGAFGIDDAVIKRAN
                     FKWSLSQLVFPHQLVRLILSEQVYRACTILKNEKYHHK"
     assembly_gap    1116..1243
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1493..>1984)
                     /locus_tag="PK977_11690"
     CDS             complement(1493..>1984)
                     /locus_tag="PK977_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18825.1"
                     /translation="IWVDPRLDTVNFSSGDSFPLSWKSYAEAKNFEFFLYSSDNISQP
                     IFKTTVSKLKIPIADFINKLKPGNSYFWTAAIKGEENEELKIFNYVSKEAFAQIEKEM
                     KDQGAAFEAPAEQAYRLAFMLEDAHYLAEAYQYYTKAATLDPANALYRATLMSFKKDY
                     EIK"
     assembly_gap    1985..2100
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2101..2396)
                     /locus_tag="PK977_11695"
     CDS             complement(<2101..2396)
                     /locus_tag="PK977_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18826.1"
                     /translation="MKKLTFLILASCFTIAAVAQNNFLLYSFKGNVSVVENNVETKAK
                     VGKPLNSNATIKLTAGAAATLICNEAAMFTLTKPGTYALKNFGDSCKVSSNSVS"
     gene            complement(2419..3795)
                     /locus_tag="PK977_11700"
     CDS             complement(2419..3795)
                     /locus_tag="PK977_11700"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRF18827.1"
                     /translation="MKKAVKKFGSGAKKVSKHLTKYLYERDTIFSTLWVFIFIVALSQ
                     IPINFYFLNPLKLAMKDFDFNDLAYSKLGKGKLGSSEDSKFDKNIVIVNIADLDREGL
                     AMLIDKTASYNPKAIGLDVWMEDAREPHKDSLMREVVRRTPNLVLASRFKPSTDPERD
                     EGDYHSNYFKDSTAGTEGYVNFFNEDIATIRIWNPTTKVKIDSVTKIRYPSFAYSLIK
                     LFDSTALEHLPSKKKEIINYTRRTLQYQIVEPDALFTDNVDSAAIRGKIALLGYINID
                     PNNIDDKKFTPMNEKFYGKAVPDMNGIVVHANIISMVLAKNYIKKLPLWVVWLVAIFI
                     GWLHMSFFIRYYLENHIWFHLVAKIAQLASAIFFTYVGIYLFDRYRIKLDMKLSLIVI
                     VMAVDVIYFYEAWAVWMHRKFGYQTVFKPHHHGHGDEHGGHDSGHEKHDEEVPEEGAT
                     KKEEESSH"
     gene            3976..>5112
                     /locus_tag="PK977_11705"
     CDS             3976..>5112
                     /locus_tag="PK977_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF18828.1"
                     /translation="MRKSLSIIICSFLFCLTAAINASAQQPAPPKPRDTTIQGPQTFA
                     MIMGISDYKHVRKLNYADKDAEMFKEFLKSPAGGSLSEDNIYMLLNNQALAANFFIAG
                     FKWLKAKNLQKGDKLFIYMAGHGDAIDEDQFFYLAYDCNPAGDKNSYLVSGAIRMADV
                     KIKIAKETAKGVEVFLIMDACRSNELPGGSEGQGFFNSAISEKRAGEIVMLATGAGQE
                     SLEDANIGNGHGLFTYYLVDGLNGMADGDGDKDNKVTVEEIQKYVQKNVPAVAQQQFR
                     RKQDPYFCCTEHSSKVVSIVDQEYLKRWVEKKKQWNGGNSFGNGSWAMYKSPADTALA
                     ELYNSFYTAVKESKLTGKNSAEFYYDRMEKKYPGNSYTIDAQSTL"
     assembly_gap    5113..5128
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5129..7316
                     /locus_tag="PK977_11710"
     CDS             <5129..7316
                     /locus_tag="PK977_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18829.1"
                     /translation="FINFAQSKINLYLDCKDPASIQKIRAQIDEDEKTDEIDASLDRM
                     EKVARQEFYEVGNMLEKAISFIMPDDPDFAKSLMGRMYFFKARGYFTSGRRLVDINKA
                     FQYAYSANSAEKNAAYVLHTLASLHLDNNRIDSAINYSRWAIAVAPKWRYPYVTLAYC
                     YKMQNKPDSAIKYYRKSIDVNPENADAYVDLGHYFYSQSKTDSAIANYEKALGIEPRN
                     TYASNNIGWIYHDRKQYEKAISYFKQSIASDPKFINAYNGLGKTFFEAGQADSAKIYY
                     TKAFANYQDKSIVNVYLGNFFRELKQYDSAKTYYRIAAELDPTYEEAFNNLGKASFAL
                     KQLDSANIYYQKALVANPYSAFALINIGLVFKEMKQPDSTYKYFQQAVKTEPWNPSIL
                     NSLGVIYGQEKNYDSAKKYFRKALNVRPDYKLASNNLLRIFRELNQLDSVTNFLKGSS
                     LMDPASLGFLDEMARTFLDQKRYDSARKYYRLALSKDPVNPQLLNNMGMVFQGMKRYD
                     SAKLYIQKAMRLDPNNSIFWGNLAGIFRQLKEYDSASYYYQKQVFNKADATAQAFYTV
                     GSIMQDMKLYDSAIAYFKRAAAISPEYVEALSNICAAYIQLEQLDSAITYGRRAVALT
                     STYENAYQNLGLAFHAKQQYDSAIVYLKKSIELSPTKGKNYFQLACSYALNKQTDQAI
                     LHLKLAYELGYNNKDALFSDPDLAGLRDIKAYQELLDKYVPNWRQR"
BASE COUNT         2126 a         1488 c         1532 g         2012 t
ORIGIN      
        1 caaaaagaaa aagctgtccc gcttttttat tgatcagaaa ttatccaaaa tcgaaaaaga
       61 aaatacctgg gtactggaaa gtaacaagcg gatcatttgg gtagtaggct tccgcataga
      121 caacaggttt aagattaccg accagacaaa acaggtattg aaagtaacct tagaggttta
      181 agctgtattt gatcttcaac cagaattgtt ctgcaggctg gtaatccgat ttaagaattg
      241 cggccgcggc aataataaaa ccaataccaa acagggctcc ccatatatgg gcagagtggt
      301 tcacattgtc ttttgcctgt ttttccaaat aggtggatgc gatgaggtac aaaggaccga
      361 aaatataacc cggtattacc ggcggaataa agaagaaacc aatcttatta gtcgggtcga
      421 acaggatgcc ggcaaacact accgcactta ccgcaccgga ggcgccaagg cttcggtagt
      481 agtaatcgtt tttatgtttg ataaaagtgg gaaccaggca tacaaaaagg gccagtacat
      541 acatgccgag gtacagaaat tttcctttct ctccaaagag gataggggta gagaaacttc
      601 tttcaacaaa ctctccgaac atatacaagg agatcatatt gaaaagcagg tgaccaatat
      661 cagcatgtat cagtccgcag gtaaagaacc ggtaccattg ccctctttca gttaccgaag
      721 gcggataaaa aatcaggtca tcaatgatct tatggttgct gaaagcactg aaagatataa
      781 gcccggtgat gattatgatg ataagggtaa ttgataattc catcggctaa atgtaaagtc
      841 aaaaatacag aacggcaaat acccggtcat ttatgatgat atttttcatt ctttaatatt
      901 gtacaggccc tgtacacttg ttcggacagg ataagccgta ctaactggtg cggaaaaacc
      961 aactgagata aactccattt gaaattagcc cttttaataa cggcatcatc tataccaaaa
     1021 gcaccaccaa tcaggaagac aatgcgtttg gtactctcat tggcccttgc ctggataaat
     1081 gtagccaggc cttctgagct cagttgtttc cccctnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtccact ttatagtatt
     1261 tggagatgcg gtaagtgaat tcttccacac cggcttttac atatgcctca tgattttttc
     1321 caactgacca gaaactgaat cgcatagagg gtaaaaataa agcattcacc acttctgctt
     1381 tatttttcct tgcaatgaca ccgctacaga aatggaaagg tgccggctac ttattgccgc
     1441 aaaaaaaagt cctgcacagt ggcaggacct gtttaataag caatttgcct ggctatttta
     1501 tttcgtaatc ttttttgaac gacatcaaag tggcacggta taaggcattg gccggatcta
     1561 atgtagctgc ctttgtataa tactggtaag cctcggccag gtaatgggca tcttccagca
     1621 taaaggcgag acggtaagcc tgctcggcgg gtgcttcaaa tgcagctccc tggtctttca
     1681 tctctttttc aatctgggca aaggcctctt tgctcacata attaaagatt ttcaattctt
     1741 cgttttcttc acctttgata gcagcggtcc agaaataact attgccgggt tttagcttat
     1801 taatgaagtc agcgatcggt attttcagtt tgcttacagt tgttttgaaa attggctgac
     1861 tgatattatc gctgctgtaa aggaagaatt caaaattctt tgcctctgca tacgatttcc
     1921 atgaaagggg gaaactgtcg ccggaagaga aattgactgt gtccagcctt ggatctaccc
     1981 aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 ctgcttacag aattactgct cactttacag gagtcaccaa aattcttcag ggcataagtg
     2161 ccgggtttgg taagagtgaa catggcggct tcattacaaa tgagggtggc ggcagcgcct
     2221 gctgttaatt ttatggtagc attgctgtta agtggtttgc ccactttggc ttttgtttcc
     2281 acgttgtttt caacaactga tacgttgcct ttgaaactgt aaagcagaaa attattctgt
     2341 gctacagcgg cgatcgtaaa acaacttgcc aggataagga aggttaactt tttcatgtgc
     2401 ttctttttta tatcgaattt aatgtgatga ttcttcttct tttttggttg ctccttcttc
     2461 aggaacttct tcatcatgtt tctcatgtcc gctatcgtgg ccaccatgtt cgtcaccatg
     2521 gccatggtgg tgcggcttaa atacggtctg gtaaccaaat ttccggtgca tccacaccgc
     2581 ccaggcctca taaaaatata taacatcaac agccatgaca attacgatca gcgagagctt
     2641 catatccagt tttatccggt acctgtcgaa taaatagata cctacataag tgaagaaaat
     2701 cgccgatgct aactgtgcaa tctttgctac taaatggaac cagatatggt tttctaggta
     2761 ataacggatg aagaacgaca tatggagcca cccgataaaa atggctacca gccaaacgac
     2821 ccacaaaggt agctttttta tatagttttt ggccaaaacc attgaaataa tattggcatg
     2881 caccactatc ccgttcatat cgggtacggc ctttccgtag aacttttcgt tcattggcgt
     2941 gaacttctta tcatcaatgt tattggggtc gatattgata taccccagca gggcaatttt
     3001 gccccttata gcagcagaat ccacattatc tgtgaaaaga gcatccggtt ctacaatttg
     3061 atactgcagc gtacgtcttg tgtagttgat aatttccttc tttttgcttg gcaggtgttc
     3121 taaagcagtt gaatcgaaca atttgatgag ggaataggca aagctcgggt atcttatttt
     3181 ggtaacactg tctattttta ctttggttgt cggattccat atgcggatgg tagcaatgtc
     3241 ttcattaaaa aaattaacat acccttctgt acctgcagta ctgtctttga aataattact
     3301 gtgataatca ccttcgtctc tttcaggatc agttgaaggt ttgaagcggc tggccagcac
     3361 aagattaggt gtgcgcctga ccacttcccg catcaatgag tctttatgcg gctcccttgc
     3421 gtcttccatc catacatcta ggcctattgc ttttggatta tacgaagctg ttttgtctat
     3481 gagcattgcc aaaccttccc gatccaggtc agcaatattg acaatgacaa tgttcttatc
     3541 aaatttagaa tcctcactgc tgcctagttt cccttttcca agcttggaat aagcaaggtc
     3601 attgaaatca aagtccttca tcgccagttt cagcgggttc agaaaataaa agttgatggg
     3661 tatttggcta agtgcaacaa tgaaaataaa cacccataaa gtggaaaaaa tggtgtccct
     3721 ttcgtacagg tacttggtta agtgcttgga tactttcttg gcccctgaac caaatttctt
     3781 gaccgctttt ttcatcagaa tgatttgctg ctgatcagca gttgattaac aatgatttgc
     3841 taaagataga cagggttgca actaccttcc cctttctcat catctgtttc tcgttgtttt
     3901 ttgcccggca accggctaaa acaagcgaca ttgcaggtat tcacctgtaa atccgtaaat
     3961 tagtcggtcg aatttatgcg aaaatccctt tccataatca tttgctcctt tttgttttgc
     4021 ctgacagcgg ctatcaacgc ttctgcacag cagccggcgc cacccaaacc aagagatacc
     4081 accattcagg ggccccaaac ttttgccatg attatgggca tctcagatta caagcatgta
     4141 cgtaaactaa actatgccga taaggatgcg gaaatgttca aagagtttct caaatcaccg
     4201 gcaggtggca gtttgagtga ggataatatt tacatgttgc tcaataacca ggctctggca
     4261 gctaacttct ttatagccgg atttaaatgg ctgaaagcaa agaacctgca aaagggagat
     4321 aaacttttta tttacatggc aggccatggt gatgcgattg atgaagacca gtttttttac
     4381 ctggcctatg actgtaatcc tgccggtgat aagaacagct atctcgttag cggggccatt
     4441 cgtatggctg atgttaagat taagatcgcc aaggaaacag ccaaaggggt agaggtattc
     4501 ctgatcatgg atgcctgccg gtccaatgaa ttgccgggag gttcggaagg gcagggtttt
     4561 ttcaattctg cgatttctga aaaaagggcc ggggagattg tgatgcttgc taccggtgca
     4621 ggccaggaat ctctcgagga tgccaatatt ggtaacggcc atggattatt tacttattac
     4681 ctggttgatg gactaaatgg aatggcagac ggagatggtg acaaggataa taaagtgacg
     4741 gtggaggaaa tacagaaata tgtacagaaa aatgtacctg ccgtagcgca acagcagttt
     4801 cgcagaaaac aggatcctta tttctgttgt actgagcaca gcagtaaagt ggtaagtatt
     4861 gtagaccagg aatacctgaa acgatgggta gaaaagaaaa aacagtggaa tggcggtaat
     4921 tcctttggta atggctcatg ggccatgtat aagagccctg ctgatacggc gttagctgaa
     4981 ttatataact ccttttatac cgcagtaaaa gagagtaagc ttacgggtaa aaattcagca
     5041 gaattctact acgaccggat ggagaaaaaa tatccgggaa acagttatac tattgatgcg
     5101 caatcaacac tgnnnnnnnn nnnnnnnngt tcatcaactt tgcccaaagt aagatcaatc
     5161 tgtaccttga ttgtaaagac ccggcttcca tccaaaagat aagagcacag atagacgagg
     5221 atgaaaagac agatgaaata gatgccagcc tggacagaat ggaaaaagtg gcccggcagg
     5281 aattttatga agtaggtaac atgctggaga aagccatcag tttcatcatg ccggatgatc
     5341 cggattttgc gaaatcactc atgggccgta tgtacttttt taaagcaagg ggctatttca
     5401 ccagtggaag aaggctggtg gatataaaca aagctttcca gtatgcctat tctgccaaca
     5461 gcgcagaaaa gaatgctgcc tatgtgttac atacccttgc gtccctgcac ctggataata
     5521 accgtattga cagtgcgatc aattattccc gctgggcaat tgccgttgct cccaaatggc
     5581 gctatcctta tgtaacactg gcttattgct ataaaatgca aaacaagccg gattcggcaa
     5641 ttaaatacta ccgaaagtct attgatgtta accctgaaaa tgcagatgcc tatgtagatc
     5701 tgggccatta tttctattcg caatcaaaaa ctgattccgc cattgctaat tatgaaaagg
     5761 cattgggaat tgaaccccgg aacacttatg ccagcaataa tattggctgg atataccatg
     5821 acaggaaaca atatgaaaaa gccatcagct attttaaaca aagcatagca tcagatccta
     5881 aattcattaa tgcctataat ggattaggta aaactttttt tgaagccggg caagcagatt
     5941 ctgcaaagat ctattacaca aaagctttcg ccaattacca ggataaatcg attgtaaatg
     6001 tctacctcgg taattttttc cgggaactaa aacaatacga ttcagccaaa acgtattatc
     6061 gtattgctgc tgaattagac cctacctatg aggaagcatt caataattta ggtaaggcaa
     6121 gttttgccct gaaacagctc gactctgcca atatctatta tcaaaaggca ttagtggcta
     6181 atccatattc agcgtttgcc cttatcaata ttggactggt gtttaaggaa atgaagcagc
     6241 cggactcaac gtataaatat ttccagcagg cggtaaaaac agagccctgg aacccttcta
     6301 tcctgaacag cctgggagtt atctatggac aggaaaaaaa ttatgactct gctaaaaagt
     6361 atttcaggaa ggcattaaat gtaagacctg attacaagct agcttctaat aacctgctgc
     6421 gcatattcag ggaattgaat cagctggatt cggtaaccaa tttccttaaa ggttcttccc
     6481 tgatggatcc tgccagcctt ggtttcctcg acgaaatggc ccgtacattc ctcgaccaga
     6541 agcgttatga ttctgcccgg aaatattatc gcctggcatt gagcaaagat cctgtcaacc
     6601 cgcagttact taataatatg ggcatggttt tccagggaat gaagcgttac gattctgcca
     6661 agctgtatat tcaaaaggct atgcggcttg atccgaacaa tagtatattc tggggcaacc
     6721 tcgctggtat attccggcaa ttgaaagaat atgattccgc ttcttattat tatcaaaagc
     6781 aagtattcaa taaagcagat gccacagccc aggcatttta tacagtaggt agtattatgc
     6841 aggatatgaa actgtatgat tcagccattg cttatttcaa acgggcagca gcaattagcc
     6901 ccgagtatgt cgaagcgtta agtaatatat gcgcagcgta tattcaactg gagcaattgg
     6961 attcagccat tacatacgga cgcagagcag tagcattaac aagtacatac gaaaatgctt
     7021 atcaaaacct tgggctggca ttccatgcca aacaacaata tgattcagcc atcgtatatc
     7081 tcaaaaaatc cattgagctg agtccgacaa aaggcaagaa ttatttccag ctggcctgca
     7141 gctatgcatt gaataagcaa accgaccagg ctatcctgca cctgaagctg gcttacgaac
     7201 tggggtataa taataaagat gctttgttta gcgatcctga tttggcgggc ctgagggata
     7261 tcaaagctta ccaggaactg ctggataagt atgtgcccaa ctggcgacaa cgataatcgc
     7321 tgccttgttt ttcaagtggt tatataaaaa gaaaagcctc cgtgcggagg cttttgtgat
     7381 cccgtcagga ttataacccc ttttacatac ttttacac
//
LOCUS       DAORLS010000150         7348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5470_length_7348_cov_1.592212, whole genome shotgun sequence.
ACCESSION   DAORLS010000150 DAORLS010000000
VERSION     DAORLS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5470_length_7348_cov_1.592212"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK977_11715"
     CDS             complement(<1..515)
                     /locus_tag="PK977_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF18830.1"
                     /translation="MKTALVTGASGNLGQAVIQKFLDEGYHVTGTVVPADPVKLDVSH
                     PNLQLVEADLLNETAAAQVSQKVIESYGHIDTAVLTVGGFAMGTVAETTAADLLRQYK
                     LNVETAYNVARPVFLQMMKQGSGRIFMIGARPGIDMKNSKGMIAYGMAKSLIFRLAEL
                     MNEEAKGTNVVV"
     gene            624..2633
                     /locus_tag="PK977_11720"
     CDS             624..2633
                     /locus_tag="PK977_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF18831.1"
                     /translation="MKRSILLLLILISFYDKASAQLCTTPGQTPVTAVLVCGTESFYA
                     STPVYCGTIPIPVPCPAGFTYYNKNPNFFRMNCFASGTLGFVITPDNPTSDYSWQLFD
                     ITNTNPNEVFTNTSLFVACNWSSEPGETGASIDGTDTIVCGGGGMPLFSRMPMIREGR
                     TYLLMVTNQNNAFEGYNITFTGGTASITDPVEPHLLSARASCEGNTIVLRGNKKLQCS
                     SLAGDGSDFSISGGAVIYSAYPGDCTSPLGTDSIMIDLVCPLPIGTYTLTMNTGNDGN
                     TLMDVCNRSIPENETISFEVTALQPITMDSLQNPGGCSPAFVDLVFDKGIQCSSIAPD
                     GSDFLFTGPQPISAGFVPGACNTGSVTNRIRLLFNTPITVRGTYALSLVTGNDGNSII
                     DECGLVVNPGSPLTFDVVSPLSPVFTINNPLSCKQDSIRFFHDGNNNTNSWNWIFGDG
                     QNSSSQNPVHYYSNPGNYTATLIVKNNLCTDSSKQTIKVNGNLEAGFTVSPAICPGDT
                     LQLVNTSTGAIDRWQWDLGDGTRSTLKTPSGHRYPAVSRDVFFTITLIASSDLLQCSD
                     TLKQTVRLLSSCLIKVPTAFTPNGDGKNDFLYPLNAVKAMNLEFKVFNRYGQLVFVSR
                     DWTRKWDGTINGLPQSTGVYAWQLSYTHADTGEKVYEKGTTLLLR"
     gene            complement(2622..>3876)
                     /locus_tag="PK977_11725"
     CDS             complement(2622..>3876)
                     /locus_tag="PK977_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRF18832.1"
                     /translation="YAELGAMMPETGGIYVYFRKMFGDFFAYLYGWAAFSVINTTGVA
                     AIAFVCAQYADYFLHLPRFDTATEQSVIWHLPFIGDLRPLENFGVKSLAIAIIGGLTW
                     LNVRSVKAGSSFQVISTIIKVAVIAALVIGIFFFGNGSVDNFFYSDNAKHGTALFSGI
                     IAALTGAFFAYDGWINITFIAGEIKQPQRNIPRSLFTGVIACIIVYVLVNQAYLYVLP
                     VEKIAGSSLVASDAISLAIGNAGGAAVAAMIVICTLGAVNGNLMATTRVTYAMGKDKV
                     FLPWSGREHKKYQTPGNALWLHGGWIAVLIISGSFDMLADMMVFISWIAYGLGAVGIF
                     MLRKKMPLAERPYKIWMHPFVTLLFIIFTSLFLVITVYNDITNYLNDRQPVVNSLLGI
                     LIVTLGIPFYFYYRSKNKKEQSSAE"
     assembly_gap    3877..3909
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4288..4444
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4445..4627)
                     /locus_tag="PK977_11730"
     CDS             complement(<4445..4627)
                     /locus_tag="PK977_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HRF18833.1"
                     /translation="MISRMMLQNPNVILLDQPTNHLDLESIQSFNEQCSDYKGIVLLT
                     SHDHTFMQTVANRIIEL"
     assembly_gap    4656..4700
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4701..>5336)
                     /locus_tag="PK977_11735"
     CDS             complement(<4701..>5336)
                     /locus_tag="PK977_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HRF18834.1"
                     /translation="AVCTHVADVDRQKIKIFTGNYTFWYQSSQLMARQINDKNKKVEE
                     KRQALLDFIARFSANASKSKQATSRKKALEKLTIEEIEPSYRKYPGIIFQQLREVGNQ
                     ILNTQKLQKSVDGRLLFSDVTFTVNKGDKIALLSRDPLAVTSFFNIITGEIMADSGSY
                     EWGSTVTHAYLPQENNQYFDKDLNLMDWLRQYVPDHVKDVDEPFLRGFLGK"
     assembly_gap    5337..5349
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5350..>5819)
                     /locus_tag="PK977_11740"
     CDS             complement(<5350..>5819)
                     /locus_tag="PK977_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF18835.1"
                     /translation="ITPGERMAVLKQNQFQFDEETVLNTVMMGHTKLWSVAKERESIY
                     ALADFTEEDGIRAGELEHEFGEMGGYTAESDAATLLSELGVKEEFHQSLMKDIPSNLK
                     VRVLLAQALFGNPDILLLDEPTNGLDIETISWLENFLADYENIVLVVSHDRHFL"
     assembly_gap    5820..5906
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6157..6711
                     /locus_tag="PK977_11745"
     CDS             6157..6711
                     /locus_tag="PK977_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF18836.1"
                     /translation="MLVIFNSHAQNSKSVFVHFAFNKSVLDATARARLDSLTDSLDVS
                     DRIELHGHCDAIGTNDYNDVLSQKRVQSVKNYLLNIGWESGDILIAAGHGKRMPVTSN
                     DNPEQRSLNRRVEIKIIYGNPAASNASPPATKPPTLTEKIADSTVKSGTNLVLRNINF
                     AGGLHRFLPESYPMLEELLEAMRT"
     gene            6711..6965
                     /locus_tag="PK977_11750"
     CDS             6711..6965
                     /locus_tag="PK977_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF18837.1"
                     /translation="MIRVEGHICCNPTPADGLDNETGQFNLSEARAKAVKDYLVKNGI
                     ETKRISYKGFGHSMPLYPYPEKSIEEEKLNRRVEIRIISK"
     gene            complement(7037..>7348)
                     /locus_tag="PK977_11755"
     CDS             complement(7037..>7348)
                     /locus_tag="PK977_11755"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18838.1"
                     /translation="IQVNECADRHLLLDAFQAVFVYPNPNNTGNFNIRLNTDLYNNIG
                     VRVFNAMGQEVHSQFFAGLTYGSVVTLNIGRLTPGAYQLYIYNNQNGFIKKAVSVLYV
                     K"
BASE COUNT         1998 a         1645 c         1460 g         1910 t
ORIGIN      
        1 accactacat tagtgccctt tgcttcttcg ttcatcagtt cggccaatcg gaagatgagt
       61 gatttggcca tgccatatgc tatcatgcct ttgctgttct tcatatcaat acctggccgg
      121 gcgccaatca taaaaatgcg gccactgccc tgcttcatca tttgcagaaa aacaggtcgg
      181 gcaacattat aggctgtttc tacattcagt ttatattgcc tgagcaggtc agcagctgtg
      241 gtttccgcaa cagtgcccat ggcaaatcct cccacagtaa gaacagccgt atctatatgt
      301 ccgtaggact caatcacctt ttgcgatact tgtgcggctg cagtttcatt taaaagatct
      361 gcttcaacga gctgaaggtt ggggtgacta acatccagct ttaccggatc ggcaggaacc
      421 accgtacccg taacatgata tccttcatcc aggaacttct gaataacggc ctggccaaga
      481 ttgccggaag cgccggttac tagtgcagtt ttcatcaacc gaaaatacaa aacttcaggt
      541 ccttgctttc aacgatgttt gttttgtaac ttgtaaggtc attgaccggc aacctgtaac
      601 acctgtgttc cgtttgacgc attatgaaaa gaagcatact gctgctgctc atccttataa
      661 gcttttacga taaagcttcg gcacagttat gcaccacacc aggacaaaca ccggtaacgg
      721 cggtgctggt ttgcggtaca gagagttttt atgccagtac cccggtttat tgcggcacca
      781 ttcccatacc ggtaccctgc cctgccgggt ttacttatta caataagaat cctaattttt
      841 tcaggatgaa ttgttttgcc tccggtacac tgggatttgt catcaccccg gataacccga
      901 cttccgatta cagctggcag ttatttgata ttaccaatac caacccaaat gaggttttca
      961 ccaatacctc cctgtttgtt gcgtgcaact ggtcatcaga accgggagaa accggcgcat
     1021 caattgacgg tacggatacg atcgtttgcg gcggcggcgg tatgccttta ttcagccgga
     1081 tgccgatgat tagggaggga cgaacctacc tgctgatggt taccaaccaa aacaatgcct
     1141 ttgaaggata caatatcact tttaccggcg gcactgccag cattaccgac ccggtggaac
     1201 cacacctgct gagtgcaagg gccagttgtg aaggcaatac cattgtattg agaggtaaca
     1261 aaaaattgca atgcagctca ctggctggtg atggaagtga ttttagtata tcaggtggcg
     1321 cagtgatata tagtgcctat cccggcgact gcacatcacc gctgggtacc gactctatca
     1381 tgattgatct cgtttgtcca ttaccaatag gcacctacac acttacaatg aatacaggca
     1441 acgatggtaa tacgctgatg gatgtttgta accggagtat tccggagaat gaaacaataa
     1501 gcttcgaggt aacggccctg caaccgatta ccatggatag tttgcaaaac ccgggcggct
     1561 gctctcctgc atttgttgac ctcgtttttg ataaaggtat ccagtgcagt tccattgctc
     1621 cggatggcag tgattttttg tttaccggtc cgcaacccat cagtgctggc tttgtacccg
     1681 gcgcttgcaa caccggttca gttaccaatc gtatacgatt attattcaat acaccgatca
     1741 ccgtgcgtgg tacttatgcg ttatccctcg ttaccggcaa tgatggcaac tcaatcattg
     1801 atgaatgtgg cctggttgtt aaccccggat cgccgctgac attcgatgtc gtatcaccat
     1861 tatcaccagt atttactatc aataatccgc ttagctgcaa acaggacagc attcgttttt
     1921 ttcatgacgg aaacaacaat acaaatagct ggaactggat ttttggcgat ggccaaaaca
     1981 gttcatctca aaatccggtt cattattaca gcaacccagg caattatacc gccacattga
     2041 ttgtaaaaaa caatctctgt acagactcaa gcaaacagac tataaaagtg aacggcaacc
     2101 tggaagcagg gtttacagta tctcctgcta tttgccccgg cgatacacta caactggtga
     2161 acaccagcac cggtgctatt gaccgatggc aatgggacct gggtgatgga acaagatcaa
     2221 ctctgaaaac accttccggc catcgttatc ctgctgtcag ccgggatgta ttttttacca
     2281 ttacattgat tgccagcagc gatttattac aatgcagcga tacgttaaaa caaacagtta
     2341 ggttattaag cagttgcctt atcaaagttc caactgcctt tacacccaat ggcgatggta
     2401 aaaacgattt tctttacccg ttgaatgcgg taaaagcaat gaaccttgaa ttcaaagtgt
     2461 tcaaccgcta cgggcaactt gttttcgtca gccgtgactg gacccgcaaa tgggatggca
     2521 ctattaatgg cctgccacaa tcaacaggcg tctatgcatg gcagcttagc tatactcatg
     2581 cagataccgg tgagaaagtg tatgaaaaag gtacaaccct tttactccgc tgatgattgt
     2641 tcctttttat tttttgaacg atagtagaag taaaatggta tgccaagggt aacaattaaa
     2701 atacccagta atgaattcac aaccggctgc cggtcattga ggtaattagt aatatcgttg
     2761 tatacagtta ttaccaaaaa aaggctggta aagatgataa acagcaatgt cacaaacgga
     2821 tgcatccaaa tcttataggg tcgttcggcc aatggcattt tttttcgcag cataaaaata
     2881 cccacagcac ccagaccata ggcgatccag ctgatgaaca ccatcatatc tgccagcata
     2941 tcaaatgagc cggatatgat cagtaccgca atccagccac cgtgcaacca caaagcatta
     3001 cccggtgtct ggtacttctt gtgctctctg cctgaccatg gtaagaatac tttatccttt
     3061 cccatagcat aggtaacacg ggtagtagcc attaaattac cattgacggc acccaatgta
     3121 caaataacaa tcatagcagc tacagcagct cctcctgcat tcccgattgc taatgaaata
     3181 gcatcggaag ctaccagcga tgaacctgca attttttcta ccggcagtac atagagatag
     3241 gcctggttga ccagaacata cacaatgatg caggcaataa caccggtaaa taaacttcgg
     3301 ggaatatttc gctgcggctg tttgatctca ccagcaataa atgtgatatt gatccagcca
     3361 tcgtatgcaa agaaagcccc ggtcaatgct gcaatgatac cactgaacaa tgccgttcca
     3421 tgttttgcgt tgtctgaata aaagaaatta tctactgagc catttccaaa aaagaaaata
     3481 ccaataacca atgctgcaat caccgccacc ttaataatgg tagaaataac ctggaatgaa
     3541 cttccggcct ttacgcttcg tacgttcaac caggttaaac caccaataat cgcaatagcc
     3601 agcgatttta ctccaaagtt ctccaacgga cggaggtcac cgataaatgg cagatgccaa
     3661 atgacagatt gttccgtggc tgtatcaaac cttggcaggt gcaaaaaata atcagcatac
     3721 tgtgcacata caaatgctat tgctgctacc ccggttgtat ttatcactga aaaagcagcc
     3781 cacccataga ggtaagcgaa aaagtcgccg aacattttac ggaagtatac atagatgcca
     3841 cccgtttccg gcatcatggc acccaactct gcatatnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnc agggagaaga gtcctgctat tatccatacc aatgtcaacc atatcggtga
     3961 acctaactga gccgccatgc tggccggctt cataaataca ccggaaccga tgatgctgcc
     4021 gataatgata gaagtggcag accagaaacc tatcttcttg gcaagtgatg aggtggtgga
     4081 cataaaaggt aagataacaa aaagccccgt tttagaacgg ggcttttaaa atcatttatc
     4141 tcatttggct tcaacccgaa tctcccgtat tactatcggg acataccctc cggccggata
     4201 caagtttttc ccggaaggat taacaggtac ggtaggtgtt ggattccggt tataaaagaa
     4261 cagttggctt atgacagcta aagtagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnncagttc aatgatacgg ttagctaccg tttgcatgaa tgtatggtca tggctcgtta
     4501 gtaaaacaat gcccttataa tccgaacact gttcgttgaa actttgaata ctttccaggt
     4561 caagatgatt ggtgggctga tccagtaata tcacattcgg attttgcaac atcatccggc
     4621 tgatcatgca tcgaactttt tcacctccac ttaatnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn ttttacccag aaaacctctc aggaaaggct catctacatc
     4741 tttcacatga tcgggaacgt actggcgcag ccagtccatc aggttcaggt ctttatcaaa
     4801 atactggttg ttttcctgcg gcagataagc atgggttacg gtgcttcccc attcgtaaga
     4861 accactgtct gccattattt ccccggtaat gatgttgaaa aaactggtga cagccagtgg
     4921 gtcacggctc agcaaggcta ttttatctcc cttattaacc gtaaaggtta catcgctaaa
     4981 aagcagacgg ccatcaaccg atttttgcag cttttgtgta tttagtatct ggttacccac
     5041 ttcccttaat tgctggaaga tgataccggg atatttccgg taactgggtt ctatttcctc
     5101 aatagttaac ttttccaatg ccttcttcct agatgtcgcc tgtttactct tagaagcatt
     5161 agcactaaac cgggcaatga agtccaacag ggcctgccgc ttctcttcta ctttcttatt
     5221 cttatcattg atctggcggg ccatcaactg agatgactgg taccagaagg tatagttgcc
     5281 ggtaaatatt ttgatctttt gcctgtccac atccgctaca tgtgtacaca ctgcatnnnn
     5341 nnnnnnnnng aggaagtgac ggtcgtggct caccactaat acgatgttct cataatccgc
     5401 caggaaattt tccagccagc tgatggtttc aatatccaga ccgttggtag gctcatccag
     5461 caatagtata tcagggtttc caaaaagtgc ctgtgccagc aatacccgaa ccttcaggtt
     5521 ggatgggatg tctttcatca gcgattggtg aaattcttcc ttaactccga gctcacttaa
     5581 caaagttgcc gcatcacttt ccgcggtgta gccacccatt tcaccaaatt catgttccag
     5641 ttcccctgcc cggataccgt cttcttcggt aaagtcagcc agcgcataaa tagattctct
     5701 ttctttagct actgaccaca gtttggtatg ccccatcata accgtattca gcacggtttc
     5761 ctcatcaaac tgaaactggt tctgtttcaa tacagccata cgttcgccgg gtgtaatctn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnngttg cctttggtaa aattcaggtt cacttcttcg
     5941 aacaacaccc gtttgccata ggccagtttc aggtctttta cactgatcat gagtcaaatt
     6001 ttgaggccgc aaaggtaggt taaaacaggc ggctattttc ctctattaca agaaaaatga
     6061 tctaatcccg ttattagttt gatcctttct aattaactga taatgactgc cacccggaca
     6121 ttaaaatttt gcaggccctt cattttgctt ttgtttttgc tggttatttt taacagccat
     6181 gcacagaaca gcaaatctgt tttcgttcat tttgcattta ataaatccgt gctggatgcc
     6241 actgcaaggg caaggctaga cagcctgaca gattcattgg atgtaagtga ccgcatagaa
     6301 ttacacggtc attgtgatgc aatcggtaca aacgattata atgatgtatt atcacaaaaa
     6361 agagtacaat ctgtaaaaaa ctacctgctg aatattggtt gggaatccgg ggatatactg
     6421 atagcagcag ggcatggtaa acgtatgccg gttaccagca atgacaaccc ggaacaaaga
     6481 agtctgaacc gaagagtgga aataaagatc atttacggaa accctgctgc ttcaaacgct
     6541 tctccaccgg ccacaaaacc tcctacctta acagagaaga ttgcagattc aacagtgaag
     6601 tccggaacta accttgtact aagaaatatc aattttgccg gaggccttca tcgttttttg
     6661 cctgagtctt acccgatgct ggaagaattg ttagaggcta tgcggactta gtgatacggg
     6721 tagagggaca tatttgctgc aatcccactc ctgctgatgg gctggataat gaaacaggcc
     6781 aatttaatct ctcagaagca agggcgaaag ctgtaaaaga ttacctggta aaaaatggta
     6841 ttgaaacaaa aaggataagt tataaaggat ttggtcattc aatgccactg tatccttacc
     6901 cggaaaaatc aatagaagaa gaaaaattaa acaggcgggt agagatcagg attatcagca
     6961 aataatatca gaggtaaaaa ataaaaaacc gcccacttta tcggggcggt ttttttaatg
     7021 tataacaaaa cggttattat ttcacataaa gaacgctgac tgctttctta atgaaaccat
     7081 tttggttatt atagatataa agctggtaag ctcctggtgt cagtctgcct atattcagcg
     7141 taactacgct tccataagtc aaacccgcaa agaactggct atgcacttcc tggcccattg
     7201 cattaaatac ccttacacct atgttgttat acagatcagt attcaacctg atgttgaaat
     7261 tgcctgtatt gttaggattc gggtaaacaa acactgcctg gaaggcatcc agtaacaagt
     7321 ggcggtctgc acactcattc acctggat
//
LOCUS       DAORLS010000151         7339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5480_length_7339_cov_2.070566, whole genome shotgun sequence.
ACCESSION   DAORLS010000151 DAORLS010000000
VERSION     DAORLS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_7339_cov_2.070566"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /gene="infB"
                     /locus_tag="PK977_11760"
     CDS             <1..1849
                     /gene="infB"
                     /locus_tag="PK977_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRF18839.1"
                     /translation="KGKNLKAKLRREKRQELAEAGAAAEDNKLQVTEFISVSELANLM
                     DVSFAEVISKCMSLGIMVSINQRLDAEVIELVASEFGFDVEFIDMEKQMEMEEEIEED
                     DEENLKFRSPIVTIMGHVDHGKTSLLDYIRNANVVAGEAGGITQHIGAYQVELANGKE
                     ITFLDTPGHEAFTAMRARGAKVTDIAVIVIAADDAVMPQTREAISHAQAAGVPMIFAI
                     NKIDKDGANSQKIYEQLSQMNLLVEEWGGKYQSQEISAKKGLNVDKLLEKILLEAELL
                     DLKANPDREATGTIIEASLDKGRGYVATLLVENGTLRQGDLIVSGQYFGRVKAMFNER
                     NKKEDEAGPSAPAVILGLNGAPQAGEKFKVYEDEAEAKEIAYRRAQILREQGIRTKKH
                     ITLDEIGRRLALGSFKELKVIIKGDVDGSVEALSDSLQKLSTQEILVSVIHKGVGQIN
                     ESDIVLAEASDAIVIAFNVRPSLQASRLAENAGIEIKMYSIIYNAIEEVKSAMEGMLE
                     PKVQEKIVANVEIREVFKFDKATVAGCYVLDGKIKRDSKIRLIRDGIVIYPTGEGATA
                     ELASLKRFKDDAKEVQSGMECGLTIKNYSDIKVGDVVEAYEVEEVKRTL"
     gene            1906..3327
                     /locus_tag="PK977_11765"
     CDS             1906..3327
                     /locus_tag="PK977_11765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF18840.1"
                     /translation="MGGFLVKREATFGFILIMKKIITAVFCLFFALVTANAQPQSAKV
                     VADKIAAIVGDRIILQSDIRNSILDAMRQGQQVPPNAECLMMEQALISKVLMLQAEKD
                     SLPVTDEEVEAELDQKVRFYINQFGSQEALEEVAGKTIYQIKDDARESVKEQKLAAAM
                     QRKIVDNVRITPNEVKAFFERIPKDSLPFYETELEIGQIIVYPKASRDLEMYIFDEMQ
                     NYKKQIEGKKTTFEQLAKQVSEDPGSKDRGGQYQVNRNEKTWDPIFLSTAFRLKEGEI
                     SPPVKSKFGYHIIQMVQRNGDEAIVRHILRIPPITQTEIKQAITKLDSVRAKVIAGTL
                     TFNEAASKYSDDESAKFAGPFIMNRDGSTFVTIDMLDKEMVTMLDKMKIGEISQPVPF
                     EAEQGKKGVRIIFLKSKSEPHRMNLQDDYSRISQFALEEKKSKTLDKWIKGKLPTYYI
                     MVDAGTSKECPQLEKYSTDKKSF"
     gene            3493..>4081
                     /locus_tag="PK977_11770"
     CDS             3493..>4081
                     /locus_tag="PK977_11770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRF18841.1"
                     /translation="MNTSATKITEQDLHNNPLFYKNLISESLDCIILATEEGVVDFAT
                     SSISTILGFTPNEIIGKTVFDFVHPDDQLLASTTFMDEVQMNPRVKFISIRLRNKNNQ
                     YTWCLVRGHNMLNNQYVGKMVIYFSDDSLRKAAEDALVEKEKKLRMQATILNNVTDLI
                     VTTDMNRMITSWNKVIERLSGITEEEAIGRRFREVI"
     assembly_gap    4082..4091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4092..>4570
                     /locus_tag="PK977_11775"
     CDS             <4092..>4570
                     /locus_tag="PK977_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF18842.1"
                     /translation="DYFPFTHDQVAEIVFKEGIWKGEVSFTGSDKEKKYLLHTISLLR
                     DEHGVAIGLLGVGKDITERKKAEASLQKSELFYRNLISNSLDGIIMTNAEGVITYSGP
                     SVTHVSGFKPENLLGRYVLEFVHPDDKDQAKKAFAEEASKEDRGNYFEFRILNAQGN"
     assembly_gap    4571..4607
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4608..5362
                     /locus_tag="PK977_11780"
     CDS             <4608..5362
                     /locus_tag="PK977_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF18843.1"
                     /translation="DPVFKSMVIYFSDITERRKLSQQLIDQEIQKQKLLTEATITGQE
                     KERQQIGKELHDNINQHLTTARLYLEVANEKATGEMQELIRLSLKNISGMIKEIRTLS
                     QSLVPPTLGDIGLVESIQELCDSLKRAHTFSIDFVSRHFEESAVTENMKLMLFRIIQE
                     QINNIIRHSHATEVHIKLQSDAESVILFIQDNGTGFDQEKTRKGMGLNNIASRAVLLG
                     GKADIKAEPGQGCILTIELPLSDGRRKNDIKL"
     gene            5381..7228
                     /locus_tag="PK977_11785"
     CDS             5381..7228
                     /locus_tag="PK977_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRF18844.1"
                     /translation="MSDPIKHECGLAFIRLRKPFSYYQQQYGTVLWGLNKLYLLMEKQ
                     HNRGQDGAGVAAVKLNVEPGYPFLHRFRSAAPQPIADVFRKIGSEVDELAKFNPDIKN
                     HPGLMKGHISFLGELLLGHLRYGTQGKNNAEFCHPFLKRDTIPARNLALAGNFNLVNT
                     EELFSLVNIDPGEFQRQSDLAAMMEVIHHFLIKADEASPHHMDIKAVLKKAILLFDGG
                     FHVGGMTGNGIGFVFRDAHGIRPSYYYINDEVIVAASERAAIRTAFNVGENEVQELMP
                     GQALIVNESGEVDISQIVEAKERKACSFERIYFSRGSDEKIYRERIALGYNLSEQVLH
                     AIDHDLKNTIFSFIPNTAETAFYGMLKGMEDYLNKIKIERILSWNKDFDTEKLSEMIN
                     RRIRIEKIAIKDVKMRTFITEDVSRNEMVQHVYDITYGTVRPGQDTLVVIDDSIVRGT
                     TLKESIIRMLSRLKPKRIVIVSSSPQIRYPDCYGIDMSKMGDFIAFNAAVELIKEKGK
                     TDVLKTLFDKCKELQRNNQLHTENVVKQLYKQFPIEEISAKIAQLITPPGLNIPVDVI
                     FQSIEDLHKSCPNNLGDWYFTGNYPTPGGNKVVNKAFMNYMEGRNIRGY"
BASE COUNT         2317 a         1311 c         1753 g         1911 t
ORIGIN      
        1 aaagggaaag aacctgaagg ccaaattgcg ccgggaaaaa agacaagaac ttgcagaagc
       61 cggagctgca gcagaagata acaagctgca ggtaaccgaa ttcatcagtg taagtgaatt
      121 ggctaacctg atggatgtaa gctttgctga agtgatcagc aagtgtatga gtttgggtat
      181 aatggtttca attaaccagc gattggatgc cgaagtaatt gaactggtgg caagtgagtt
      241 tggttttgat gtggagttta ttgacatgga gaagcaaatg gagatggaag aggagataga
      301 agaggatgat gaagaaaatc ttaagttccg ttctccgatt gtaactatca tgggccacgt
      361 tgaccatggt aaaacttcat tacttgacta catccgtaat gccaatgtag tagccggtga
      421 ggcaggtggt attacacagc atattggcgc ctaccaggtt gaattggcaa atggaaaaga
      481 aataactttc ctcgataccc ccggtcacga agcctttacg gccatgaggg caaggggtgc
      541 taaggttaca gatatagctg ttatcgttat tgcggctgat gatgctgtga tgcctcaaac
      601 gagggaggct atcagccatg cacaggctgc cggtgtacca atgatattcg ctataaacaa
      661 aatcgataaa gacggggcga actctcagaa gatttatgag cagctgtcac aaatgaactt
      721 gctggtagaa gaatggggcg gtaagtatca aagccaggaa atatctgcta aaaaaggatt
      781 gaacgttgat aagttgctgg agaaaatatt gctagaagcg gaactgcttg atctgaaagc
      841 aaaccccgac cgtgaagcta ccggaactat cattgaagct tcactggata aaggtcgtgg
      901 ttatgtggcc acactgcttg tagaaaacgg aacattgcgc cagggtgatt tgattgtcag
      961 tggccagtac ttcggaagag tgaaagccat gttcaatgaa cgtaataaaa aagaagatga
     1021 agccggtcct tctgcaccgg cagtaatatt gggactgaac ggagctccac aggccggtga
     1081 aaagttcaag gtttacgaag atgaagctga agccaaagaa atagcctacc gccgtgccca
     1141 gattttaaga gagcagggta tccgtaccaa gaagcatatt acactggatg aaatcggccg
     1201 tcgtttagca ttaggcagct tcaaagagtt gaaagttatt atcaaaggtg atgtggacgg
     1261 atcggtagag gctttgagcg actctctgca aaaactttct acccaggaaa tattggtaag
     1321 tgtaattcat aaaggggttg gacagatcaa tgaaagtgat attgtattgg ctgaagcatc
     1381 tgatgccatt gtaatagcgt tcaacgttcg tccttcgctg caggcctcaa gacttgccga
     1441 gaatgcaggt attgagatca agatgtattc gatcatttac aatgctatcg aagaagtgaa
     1501 aagtgcgatg gaaggtatgc tggagccgaa agtacaggag aagattgttg ccaacgtgga
     1561 aattagagaa gtattcaaat ttgataaggc cactgtagcc ggatgttatg tactggatgg
     1621 aaaaatcaag agagattcca agatccgcct gatccgtgat ggtattgtaa tttatcctac
     1681 aggtgaagga gctactgctg aattagcttc tctcaaacga tttaaggatg atgcgaaaga
     1741 agtacagtct ggtatggaat gtggattgac catcaagaac tatagcgaca taaaagttgg
     1801 tgatgtagtc gaagcctacg aagtagaaga agtaaagaga acattgtaag cttacagcta
     1861 ttagctgaga gcttcctgta atcaaacttt tgttaaatag acaacatggg cggcttcctt
     1921 gttaaaaggg aagctacttt tggatttata cttattatga agaaaatcat tactgctgtt
     1981 ttttgtttgt tttttgcctt agtaacggcg aatgcccagc cacagtcggc aaaagtggta
     2041 gctgataaaa tagctgctat agttggcgac agaattattc tgcaatcaga tatccgaaac
     2101 tctatattgg atgccatgcg gcagggacag caggttccgc ccaatgcaga atgcctgatg
     2161 atggagcaag cattgatctc caaagtgctg atgttgcagg cagaaaaaga ttcattgccg
     2221 gtaacagatg aagaagtaga agctgagctg gatcagaagg tgagatttta tataaaccag
     2281 tttggcagcc aggaggcact ggaagaagta gccggaaaaa ccatttacca gattaaagat
     2341 gatgccaggg aatcggtcaa agaacaaaaa ctggcagctg ccatgcagcg gaagattgta
     2401 gacaacgtac gtataacgcc caatgaagta aaagcttttt ttgagcgaat tccaaaagac
     2461 agcttgccat tttatgaaac ggaactcgaa ataggacaga ttatagttta ccctaaagca
     2521 tcccgtgacc tggaaatgta catttttgac gagatgcaga attacaaaaa gcagatagag
     2581 ggtaagaaga ccacattcga gcagcttgcc aagcaggtat ctgaggatcc gggaagtaaa
     2641 gaccgcggtg gccagtacca ggtgaacagg aatgagaaaa cctgggaccc tatttttctt
     2701 tccacggcct ttcgtttaaa agaaggtgaa atatctcccc cggtaaaatc aaaattcggt
     2761 tatcacatca ttcagatggt gcaaagaaat ggtgatgaag ctatcgtccg tcatattctg
     2821 agaataccac cgattactca aactgaaatc aaacaagcta ttacaaaact tgatagtgtt
     2881 agggcaaaag tgatagcagg cactttgacc tttaatgaag cagcctccaa atacagtgat
     2941 gatgagtcgg ccaagtttgc tggacccttt atcatgaacc gggatggttc aacattcgta
     3001 acaattgata tgctggataa ggaaatggtg actatgctcg ataaaatgaa aataggcgaa
     3061 atatcacagc ctgttccttt tgaagctgag cagggcaaga agggagtaag gatcattttc
     3121 ctgaaatcca agtcagaacc ccaccgtatg aacctgcagg atgattatag caggatatct
     3181 cagtttgcac tcgaagagaa gaaaagcaag acacttgaca aatggataaa agggaaactg
     3241 cccacttatt atatcatggt tgatgcaggt acttctaaag aatgtcctca actggaaaag
     3301 tatagtacag ataagaaatc cttttgatgt ttaataaata ttacatgtga aagccaatac
     3361 tcaacggtat tggctttcac ttatttaagg gtagttttgt ttttaatatc tgataaatcc
     3421 ttaattcccc caattattat taataatttt agctgactaa ttggtcattt ttaatatcat
     3481 cattactgcg ctttgaatac ttctgctacc aaaatcacag aacaggattt gcacaacaat
     3541 cctttgttct ataaaaatct gatcagcgaa tcactggatt gtataatact tgctactgaa
     3601 gaaggggtag ttgattttgc tacttcatct atttcaacga ttctagggtt tactccgaat
     3661 gaaattatcg gtaaaacggt atttgatttt gtacatccgg atgatcagtt gcttgccagc
     3721 actactttta tggacgaagt gcagatgaac cccagagtta agttcatcag tatccggctt
     3781 cggaataaaa ataatcaata cacctggtgc ctggttcggg gacacaacat gctcaacaat
     3841 cagtatgtag gtaaaatggt gatttatttt tccgatgatt ctctacgtaa agcagcagaa
     3901 gatgcattgg ttgaaaaaga gaagaagtta cggatgcagg ccactattct gaataacgta
     3961 actgatttga ttgtaaccac ggatatgaac aggatgatta catcctggaa taaagtgatc
     4021 gaaagactaa gcggtatcac agaggaggag gcaataggcc ggcgtttcag agaggtcatt
     4081 gnnnnnnnnn nagactattt tcctttcact catgaccagg tagcagagat tgtctttaaa
     4141 gagggcattt ggaagggtga agtgtctttt accggaagtg ataaggagaa aaaatatctt
     4201 cttcatacta tatctctttt acgggatgag catggggttg caattggttt gctgggtgta
     4261 gggaaggata ttacagagcg aaaaaaagca gaggcaagcc tgcaaaaaag cgaattgttc
     4321 tatcgtaatc ttatctccaa ttcactggat ggtattatta tgacaaatgc agaaggagtc
     4381 attacttaca gtggcccgtc tgtaacacat gtatctggat ttaagcctga gaacctgctg
     4441 gggcgttacg ttctcgaatt tgtgcacccg gatgataaag atcaggcaaa gaaagccttt
     4501 gcagaagaag cttctaagga agacaggggt aattatttcg agtttaggat tttaaatgcc
     4561 cagggtaatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg accctgtttt
     4621 taagtcaatg gttatttact tttctgacat tacggaacga agaaagctgt cccagcaatt
     4681 gattgaccag gaaatacaaa agcaaaaact actgacagag gctaccatta caggccagga
     4741 aaaagaaagg cagcaaattg gaaaagagct gcacgataat atcaaccagc acctgactac
     4801 tgcgaggctg tatctcgaag ttgccaatga aaaagcaaca ggggaaatgc aggaattgat
     4861 caggctttct cttaaaaata ttagcgggat gattaaagag atcaggacac tttcccaatc
     4921 acttgtacct cccactcttg gtgatatcgg gcttgtggaa tcgatacagg aactatgtga
     4981 ttcactaaaa agggctcata ctttttctat tgattttgtt tcccggcatt tcgaggaatc
     5041 ggccgtaaca gaaaatatga aactcatgtt gttccggatt atccaggagc agatcaataa
     5101 tatcattcgc cattcccatg ctacggaagt tcatatcaaa ttacagtccg atgcagaatc
     5161 agtcattctc tttattcagg ataatggcac tgggtttgac caggaaaaga cccgtaaagg
     5221 catgggtttg aataatattg ccagccgggc agtcctgctg ggtggtaagg ctgatatcaa
     5281 ggcagagcct ggtcagggct gtatactcac tattgagttg ccgctatcag atggtaggag
     5341 gaaaaatgat ataaaattat agcctacctt tgcacactcc atgagcgatc caattaaaca
     5401 tgaatgcggt cttgcattca tccggttaag aaagcccttt tcttattatc agcaacagta
     5461 tggaacagtg ctgtgggggc tcaataagct ttacttgttg atggaaaaac agcataaccg
     5521 cggacaggat ggtgcggggg tggctgctgt caagctgaat gtagaaccgg gttatccttt
     5581 cctgcatcgt ttcagaagtg cagcaccgca accgatagcg gatgttttcc ggaaaatcgg
     5641 aagcgaggta gatgaactgg caaaatttaa cccggatatt aagaaccacc ctggtctcat
     5701 gaagggacat atttcctttt tgggtgaatt attacttggc catcttcgct acggcacaca
     5761 gggaaaaaac aacgcagagt tttgtcatcc ttttttaaaa agggatacaa taccggcaag
     5821 aaacctggca ttggccggaa atttcaatct tgtaaatacg gaggagcttt tttcattagt
     5881 taatatagac ccgggagagt ttcaacgaca aagcgacctg gcggctatga tggaagtgat
     5941 ccatcatttt ctgatcaagg cagatgaggc ctctcctcat catatggata taaaagcggt
     6001 gcttaaaaag gcaatactgt tgtttgatgg tgggtttcat gtgggtggaa tgacaggtaa
     6061 tggtattggc tttgtctttc gtgatgcgca tggcataagg ccgtcttact actacatcaa
     6121 cgatgaagtg atagtggctg cttctgaaag agcagctata cgtaccgctt ttaatgtagg
     6181 ggaaaatgaa gtgcaggagc tgatgccggg ccaggcactg atcgtaaacg aaagcggtga
     6241 agttgatatt tcacagattg tagaagctaa ggaaagaaag gcctgcagtt ttgaacgcat
     6301 ttacttttca agaggaagtg atgaaaagat ctacagggaa agaatagcat tgggttataa
     6361 cctgagtgaa caggtactgc atgctattga tcatgatctg aagaatacca ttttctcttt
     6421 catacccaat acagcagaaa cagcttttta tggaatgctg aaaggtatgg aggactatct
     6481 gaacaagata aaaatagaaa gaattcttag ctggaataaa gacttcgata cggagaagct
     6541 tagtgaaatg atcaaccggc gtatccggat tgaaaagatc gctatcaagg atgtaaaaat
     6601 gagaacattc attacggaag atgtaagccg taatgaaatg gtgcagcatg tgtatgatat
     6661 cacctatgga acggttcgac ccgggcagga caccctggta gtaattgacg attcaatcgt
     6721 aaggggcacc acgctgaaag aaagtattat tcgtatgctt tcacggctaa aaccgaaaag
     6781 gattgttatc gtttcttcct cgcctcaaat acgctaccct gattgttatg ggatcgatat
     6841 gagcaagatg ggtgatttta ttgcatttaa tgctgcagta gagctgataa aggaaaaagg
     6901 gaagactgat gtactgaaaa ccctttttga taaatgtaaa gagctgcagc gcaacaatca
     6961 attacataca gagaatgtgg tgaagcagct ctataaacag tttcctattg aagaaatttc
     7021 tgcaaagatt gcccagctga ttaccccgcc gggattgaat attcccgttg atgttatttt
     7081 ccaaagtatt gaagacctcc ataaatcctg tcctaataat cttggggatt ggtattttac
     7141 cggtaactat ccaactccgg gcggaaacaa ggtggtcaac aaggccttta tgaactacat
     7201 ggaaggccgt aatattagag ggtattaagc ccatattcat ttatctttca gcaaattgtc
     7261 gcacatgaaa acattgctac ttgtacgcca tgccaaaagc gattgggaaa acccggcgtt
     7321 agaagatttt gatagaccg
//
LOCUS       DAORLS010000152         7194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5630_length_7194_cov_1.870010, whole genome shotgun sequence.
ACCESSION   DAORLS010000152 DAORLS010000000
VERSION     DAORLS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_7194_cov_1.870010"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PK977_11790"
     CDS             complement(<1..469)
                     /locus_tag="PK977_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRF18845.1"
                     /translation="MKVAVVGATGLVGTKMLQVLAERNFPVTELVPVASEKSVGKEVE
                     FRGNKYKVVSMTDAIAARPAVAIFSAGGGTSLEWAPKFAESGITVIDNSSAWRMDPTK
                     PLVVPEINADVLTVNDKIIANPNCSTIQMVVALNPLYKKYGIKRIVVSTYQSVT"
     assembly_gap    819..1035
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1036..>1259
                     /locus_tag="PK977_11795"
     CDS             <1036..>1259
                     /locus_tag="PK977_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18846.1"
                     /translation="AAMSVFGTVISVTSFPSLDNDGDQLFLRAANGRTIHAVAYTSAW
                     YQNELKKEGGWTLEMADTKSPCAGISNWKA"
     assembly_gap    1260..1281
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1282..2470
                     /locus_tag="PK977_11800"
     CDS             <1282..2470
                     /locus_tag="PK977_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18847.1"
                     /translation="GSPGKKNSVDAVNNDAAAPQLKRAYTTDNTTVILVFDEPVDSLI
                     GATLSNYSIDGGLTLISATTLAPLFNTVQIRTATAMSANTVYTITASNIKDCKGNSIG
                     SANKARVGLPVDAAPGEWIINEILFNPRSNAFDYVEFYNNSNKIFDASKLFIANRNSS
                     GVVSSIKALSPVPFYVFPGDYIVETENADNLSMQYLVKNPDNVLIISSPPSFPDDEGT
                     VVALNFQGNVIDEVKYKDDWHFKLIDIAEGVALERIDPNGASQDAANWTSAASTAGYG
                     TPTYQNSQYKRIQIINATIEVTPKVFSPDNDGRDDIATIQYAVTEPGYVANITVFDPS
                     GRPVNLVRNGTMGLNGYWNWDGLDDKGLKLPVGSYVVFTEIFNLQGKKSSFKNVVVLA
                     RKL"
     gene            2543..2887
                     /locus_tag="PK977_11805"
     CDS             2543..2887
                     /locus_tag="PK977_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF18848.1"
                     /translation="MNKPGYFYIMSNAHNKVLYCGATDDLYRRVNEHKKGVYTNSFTS
                     RYNINKLVYYEVFSLTADAFKREKQVKGGSRLKKIKLIESINPDWTDLTEKIFQNKGE
                     ELNRIISLLRPR"
     gene            complement(2929..3852)
                     /locus_tag="PK977_11810"
     CDS             complement(2929..3852)
                     /locus_tag="PK977_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRF18849.1"
                     /translation="MSLITVGTMAFDAIETPFGKTDRIVGGSATYVAYAASNFVKPVQ
                     QISIIGNDFPEEELAELRSRGVWLDGVEVVPDKKSFFWSGRYHEDMNSRDTLVTDLNV
                     LADFDPKIPDSYQGAEFLMLGNLAPKIQLNVIQELKQRPKLIAMDTMNFWMESAMSEL
                     KIVLKYVDMLIVNDAEARQLTGLFSLVKAAKAILQMGPKYLIIKKGEHGALLFQDDEV
                     FFAPALPLEDVFDPTGAGDTFAGGFIGHLAKTGDISFDNMKRGIIVGSAMASFCVEKF
                     GPTRLKEVTAEDISGRIQQFKDLVSFDIELV"
     gene            4066..4659
                     /locus_tag="PK977_11815"
     CDS             4066..4659
                     /locus_tag="PK977_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF18850.1"
                     /translation="MAKAQRQKASKKGLILKKAAAMFREKGFAATSMRDLAEAVGIEA
                     ASLYNHIRSKNEILEAICFDVANVYNTTIEAIESSSHKSITKIEQLLRFHIKQMTGNY
                     EAVYVSDREWKHLEEPYLSNFQNQRRSYRKKFASIIEDGIAKNEIRKIDAPTAVLIML
                     HAVSGIESWHRSKARISAQELEDNMVMIMIDGLRKQS"
     gene            4663..5742
                     /gene="paaK"
                     /locus_tag="PK977_11820"
     CDS             4663..5742
                     /gene="paaK"
                     /locus_tag="PK977_11820"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRF18851.1"
                     /translation="MSIHFHPVPVKEIKKETSDCVSVVFDVPEELKEVFAFRQGQSLA
                     MRTKINGHEVRRTYSICSSPLDNQLRVAIKKVKGGIFSSYANDRLKKGTVIELMPPVG
                     KFYTELNPANKKNYTAFAAGSGITPVLSIIKTTLATEPESSFTLVYGNRNRSSIIFFE
                     ELEALKNKYMNRFNLVHILSRERTDTKINFGRINTDKLNELSGLLDYTNTDEFFICGP
                     EEMIFSVKDYLEAKGIDKKKIHFELFTSPGQKKHTVTDDDSWLITGPVSNITVKLDGR
                     TFDFELSLNGNATILDAALKQGADLPYACKGGMCCTCKAKLVEGEVAMDVHWGLEEEE
                     VEKGYILTCQSHPKTEKVVVDFDAK"
     gene            complement(5810..>6839)
                     /locus_tag="PK977_11825"
     CDS             complement(5810..>6839)
                     /locus_tag="PK977_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18852.1"
                     /translation="PEDPMAGFVFVQDTLNPEYGHGFVNFTIKPKTNAQTLDTIDARA
                     FIVFDDNDTIPTNLHKNTIDALPPVSQVNAITGNGTNPITLTWTGADDVNGCGIDYYT
                     VYVSTDLVNYNVLFPEIRRTDTTFSLPTGFNYCFFVLATDRVGNKEILRQGVTQCSTI
                     GGPLPVTWLYFNGTNEGKNNLLKWATGTEQNSKEFRLERSLNGTDYSQIAVIPAAGNS
                     TTERRYDYTDQRIDRLNSRVMFYRLKQVDLDDRFRYSNIVRLNYNKEGNTPTIVYPNP
                     TRGVINILVGDRSVIGTEAIVSDVNGRIMKKVIITAANQQIDLSAYSNGLYLIRLSND
                     EVLKVMKQ"
     assembly_gap    6840..6849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6850..>7194)
                     /locus_tag="PK977_11830"
     CDS             complement(<6850..>7194)
                     /locus_tag="PK977_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18853.1"
                     /translation="DPNEIKGPDGQPDKAWISVKNKIPYTIFFENDTTATAPVKYLKI
                     TSPVEPKQDAATLELGNVGFNNFSFDIPSGNSFYYQRLDARDSAGLFIDMTAGYDIVT
                     NNIFWEFQAIDPI"
BASE COUNT         2045 a         1519 c         1433 g         1948 t
ORIGIN      
        1 ccgttacact ctggtaagta cttactacaa ttcgtttgat accatatttt ttgtacagtg
       61 ggtttaaagc cacaaccatt tgaatagtgg aacaattcgg attggcgatg atcttatcat
      121 ttacagtcaa tacatcggca ttgatctcag gtactaccaa tggttttgtg ggatccattc
      181 tccacgccga tgaattatcg ataaccgtaa tcccagattc agcaaatttg ggtgcccatt
      241 ccagcgaagt gcctcctccc gcagaaaaaa ttgccacagc aggtctggca gcaatagcat
      301 ctgtcatgct caccacttta tatttatttc ccctgaactc tacttcctta ccaactgatt
      361 tttcagaagc taccggcacc agttctgtaa ccggaaaatt tctttcagcc agcacctgta
      421 acatttttgt acctacgagg ccggtagccc ctactactgc cactttcatt aatctcagtt
      481 ttattcactt ccattctgta cttagcaatt agaagtgagg ttaaaaaata aggccctccg
      541 tctggaaggc ctgctaaact tttatgttga cacatacata acgctgcaaa ccttccccta
      601 ggaatgtttc ttattgcgtt ttgtatgttt cagtatcatc attgcggtgc aaaaataacc
      661 gggcagccgg ttacaaccaa aaaatcatca aaaattgtgg cattcagaat tttgaaatca
      721 ttatttatct tgacataatg aaaccaacct tatccattct gctgctgatc atacttacac
      781 ctgtttgttt actctcccaa aacaggtatg atgtagtgnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnngcagc catgtccgta ttcggtactg ttatttcagt aaccagtttt
     1081 ccatcgctgg ataatgacgg agaccagtta tttctgagag ctgccaacgg ccgaacgata
     1141 catgctgtag cttatacttc tgcctggtac cagaatgaac taaaaaagga aggtggctgg
     1201 acactggaaa tggctgatac caaaagcccc tgcgccggaa tcagcaactg gaaagccagn
     1261 nnnnnnnnnn nnnnnnnnnn ncggctcacc aggaaagaaa aattccgttg acgcagtaaa
     1321 taatgacgca gccgctccgc aattaaaaag agcttacact actgataaca caaccgtcat
     1381 acttgtattt gatgaacccg tcgatagttt gattggggct accctatcca attattccat
     1441 tgatggtggc cttaccttga tcagtgccac tacccttgcg ccgcttttta atacagtaca
     1501 aattcgaact gcaacggcta tgtctgcaaa cacagtttac accattactg cttccaatat
     1561 taaggattgc aaaggcaata gtattggcag tgccaataaa gcccgggttg gtttacctgt
     1621 tgatgctgcg cccggagaat ggatcatcaa tgaaatatta ttcaaccccc ggagcaatgc
     1681 gtttgattat gttgagttct ataataacag taacaaaata tttgacgcat caaagctttt
     1741 tatcgccaac cgcaacagca gtggtgtagt cagttcgata aaagcattaa gccctgtacc
     1801 tttttatgtt ttcccgggtg attatattgt cgaaacggaa aatgccgata atctttccat
     1861 gcaatacctg gtaaaaaatc ctgataatgt attgatcata tcttcccctc cttcttttcc
     1921 ggatgatgaa ggaacagtag tggcactaaa cttccagggc aatgttattg atgaagtgaa
     1981 gtataaagat gactggcatt tcaaactgat cgatattgcc gaaggtgttg cgttggaaag
     2041 aattgatcct aatggcgctt cacaagatgc ggccaactgg accagcgccg cctcaacagc
     2101 aggttacggc acacccactt atcaaaattc ccaatacaaa aggatacaaa ttatcaatgc
     2161 aacgattgaa gtaactccca aagttttctc acccgataat gacggcaggg atgatatcgc
     2221 caccattcaa tatgctgtaa ccgaaccggg gtatgtggcc aacatcaccg tattcgatcc
     2281 gtccggacgg ccggttaatt tggttcgtaa cggaacaatg ggattaaatg gttactggaa
     2341 ctgggacggg ctggatgata agggtctaaa attaccagtg ggttcttacg ttgtatttac
     2401 agagatattt aatcttcagg gaaagaaatc ttcttttaaa aatgtggtag tacttgcgag
     2461 gaaattataa gacgtaagcc gcaaatcttc gaatttagtg agtacttata atttccgaag
     2521 caatctccta ttatctgtca gtatgaacaa acctggatac ttttatatta tgtctaatgc
     2581 tcataataaa gttttgtatt gcggagcaac tgacgatttg tacaggagag ttaatgagca
     2641 caaaaaagga gtttatacaa atagcttcac ctccaggtat aatatcaata aactggttta
     2701 ttatgaagtt ttttcgctta cagctgatgc gtttaagcgg gagaagcagg ttaaaggagg
     2761 ctcaaggctt aaaaaaatca agcttataga aagtataaat cctgattgga cagatttaac
     2821 tgagaaaatt tttcagaaca aaggcgaaga gctgaacagg ataattagtt tgcttcgccc
     2881 ccggtaaatt ccttttagag gaaaaaataa ttcggggggc tcgcaagttt acaccagttc
     2941 tatatcaaaa ctcaccaaat ctttaaactg ctgaatgcgg ccggaaatat cttcagcagt
     3001 aacttctttc aatcttgtcg ggccaaattt ttctacacag aaactggcca tagccgagcc
     3061 cacaatgatt ccccgtttca tattatcaaa agaaatatca cctgtcttgg ccaggtgacc
     3121 gataaagcct ccggcaaacg tatcaccggc gccagtgggg tcgaacacat cttctaaagg
     3181 caatgcaggc gcaaagaaca cttcatcatc ctggaacagt aaagcgccat gctccccttt
     3241 tttaatgatc aggtatttag ggcccatttg caaaatggct ttggctgctt ttactaatga
     3301 aaaaaggcct gtcaattgtc ttgcctccgc atcgttcaca atcagcatat ctacatactt
     3361 caaaacgatc tttagttcag acatggctga ttccatccag aaattcatcg tgtccatagc
     3421 aatcagcttg ggccgttgct tcaattcctg tattacattc agctggatct ttggcgccag
     3481 gttacccagc atcaggaatt cagcaccctg gtagctgtcg ggtattttgg ggtcaaaatc
     3541 agccagtaca ttcaaatcag taacgagtgt atccctgctg ttcatgtcct catgataacg
     3601 gccgctccag aagaaagatt tcttatcagg caccacttcc accccatcca gccatacacc
     3661 cctgctgcgt agttcagcca gttcttcttc gggaaaatca tttccgataa ttgatatctg
     3721 ctgaaccggt tttacaaaat tgctggctgc ataggctaca taggtagcag atccgcccac
     3781 aattctatcg gtttttccaa aaggagtttc gatcgcatca aatgccatgg ttccaacggt
     3841 gataagtgac atgattctta ctattaattt ttacttgaaa tttgaaaagc cgggtcagca
     3901 aatctggcta atggactcac aactattgac taccggctgc ccataaggcg ccgcaaacct
     3961 acctaaattt tagctacctg aaaaaagggc ttctgcgtat tatgaatgcc caaattgata
     4021 taactcatat tttccaataa aacatggttc gttatctttg aaactatggc aaaagcacaa
     4081 cgacaaaagg cttccaaaaa agggctgatc ctgaagaaag cagctgctat gttcagggaa
     4141 aaaggcttcg ccgccacttc tatgcgggat ttggcggaag cagtaggtat tgaagcagcc
     4201 agcctctata accatatccg gtcgaaaaac gagatattgg aagccatctg ttttgatgtg
     4261 gctaatgttt ataatacaac gattgaagcc attgaaagca gttcccataa atcaattacc
     4321 aagattgaac agctgctccg ttttcatatc aaacagatga caggcaatta tgaggctgtc
     4381 tatgtaagcg acagggaatg gaagcacctg gaagagcctt atctgtccaa ttttcagaac
     4441 cagcgcagga gttaccgcaa aaaatttgcc tctatcattg aggatggcat tgcaaaaaat
     4501 gagatcagaa aaattgatgc gccaactgca gttctcatca tgctgcatgc tgttagcggt
     4561 attgaaagct ggcatcgcag caaggcccgt atcagtgccc aggaacttga agacaatatg
     4621 gtaatgatca tgatcgatgg cttaagaaaa caatcctgat ttatgtcaat tcattttcat
     4681 ccggtaccgg taaaagaaat caaaaaagaa accagtgatt gtgtttctgt agtatttgat
     4741 gtgcctgaag aattaaaaga agtttttgct ttccgccagg gacagagcct ggctatgagg
     4801 acaaaaatca acgggcatga agtaagaaga acttattcca tttgctcttc acctttagac
     4861 aaccagttaa gggttgctat aaaaaaagtc aaaggtggta tcttctccag ctatgccaat
     4921 gacaggctga aaaaaggaac ggtgattgaa ctgatgccgc ctgttggtaa attttataca
     4981 gaattgaacc ctgccaataa aaaaaattat actgcttttg ctgccggcag tggtatcact
     5041 cctgttttgt ccattatcaa aacaacattg gccacggagc cggaaagcag tttcaccctg
     5101 gtgtatggca acagaaaccg cagctccatt attttctttg aagaactgga ggcattgaag
     5161 aacaaataca tgaaccggtt caacctcgtt catattttaa gccgggaaag aacggatact
     5221 aaaatcaatt ttggccgcat taatactgat aaactgaatg aactgtccgg cttgctggat
     5281 tataccaata ccgatgaatt ctttatttgt ggccctgaag aaatgatctt ctctgtaaaa
     5341 gattatttag aagcaaaggg cattgataag aaaaaaatac attttgagct gttcacctca
     5401 cccggtcaga aaaaacatac ggtaactgac gatgattcct ggctgataac cggccccgtc
     5461 agcaatatca cggtaaaatt agatggccgc acttttgatt ttgaattgtc tttaaatgga
     5521 aatgccacca tccttgatgc agcgctgaaa caaggcgctg accttcccta tgcctgcaaa
     5581 ggcggtatgt gctgcacctg caaagccaaa ttagtagaag gagaagtagc tatggatgtg
     5641 cattggggat tagaagagga ggaagtagaa aaaggttata tcctcacctg ccagagccac
     5701 ccgaaaacgg aaaaggtagt ggtggacttt gatgcgaaat aaaaaggccg gcaggagaac
     5761 ctggcggcct gtaaaaggag aattatcatt tcccctggca tgaaaattat tactgtttca
     5821 tcactttaag tacttcatca ttactaagcc tgatcaagta cagcccgttg gaataagcgc
     5881 tgagatcaat ttgctggtta gcagctgtta taattacttt cttcattatt cttccattca
     5941 catcactaac aatagcctcc gtaccgataa ctgacctgtc gccaaccaat atatttataa
     6001 cgcctcttgt cgggttgggg taaacaatcg tcggtgtatt gccctcctta ttataattaa
     6061 gccgtacaat attggaatac ctgaaccggt catcgaggtc tacctgtttc aaccggtaga
     6121 acataacacg gctgttcaat cggtcaatac gctgatcggt gtaatcgtaa cgccgctctg
     6181 tagtactgtt acctgcggcc ggtatcacag cgatctggct gtaatccgtt ccgttcaatg
     6241 accgctccag cctgaactct ttactgtttt gctcggtacc ggtagcccat ttcagcaggt
     6301 tgttcttacc ctcattggta ccattgaaat aaagccaggt taccggcagc gggccaccaa
     6361 tggttgagca ttgcgttact ccctgcctta atatttcttt attgcccacc ctgtctgttg
     6421 ccagtacaaa gaagcagtaa ttaaacccgg ttggcaaact gaatgtagta tctgttctgc
     6481 gtatctccgg gaacaaaaca ttgtaattca ccaggtcagt tgatacataa actgtataat
     6541 aatctattcc gcatccgttc acatcatcag cgccggtcca tgtaagggta atggggttcg
     6601 taccgttacc ggtgatggca tttacctggc tgactggtgg taacgcatct atcgtattct
     6661 tatgtaaatt ggtgggaatg gtatcattat catcaaatac gataaaggcc cttgcatcta
     6721 tagtatccaa tgtctgtgca tttgtttttg gtttgatcgt aaagttgaca aagccatggc
     6781 catactccgg gttaagcgta tcctgtacaa atacaaaacc ggccatgggg tcttcaggtn
     6841 nnnnnnnnna atgggatcta ttgcctggaa ctcccagaag atattattgg taacaatatc
     6901 ataacccgcc gtcatgtcaa taaacaaacc tgctgaatct cttgcatcta aacgctggta
     6961 gtaaaatgaa ttacccgacg gaatatcaaa ggagaaatta ttgaatccta cgttacccaa
     7021 ttctaatgtt gcagcatctt gcttaggctc caccggtgaa gttatcttca ggtattttac
     7081 cggtgcggta gcggtagtat cattttcaaa aaagatggta tagggtatct tgttttttac
     7141 acttatccag gctttgtccg gctggccgtc ggggcctttg atctcgttgg ggtc
//
LOCUS       DAORLS010000153         7072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5745_length_7072_cov_1.827989, whole genome shotgun sequence.
ACCESSION   DAORLS010000153 DAORLS010000000
VERSION     DAORLS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_7072_cov_1.827989"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>1070)
                     /locus_tag="PK977_11835"
     CDS             complement(41..>1070)
                     /locus_tag="PK977_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRF18854.1"
                     /translation="VKDIREVIETAKSQSGSVLFIDEIHRFNKSQQDALLGAVEKGTI
                     TLIGATTENPSFEVNSALLSRCQVYVLKPLEEIDLVKLMHTAIEKDEFLQKKKVELNE
                     TAALINISGGDGRKLLNLLEIVCDSSGEKVVITDKFVMEVAQRRVALYDKTGEQHYDI
                     ISAFIKSIRGSDPNAAVYWLARMIEGGEDVKFIARRMVILASEDIGNANPTALVMANA
                     TFDAVNKIGNPEARIILSQCAIYLASTPKSNASYAAIDAALAEVRKRGDLSVPLHIRN
                     APTALMKNLDYGKNYEYSHSYENNFSPQEYLPKELAGTKFYEPGKNAREEELRKFLKN
                     LWKDKYNY"
     assembly_gap    1071..1115
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1116..1312)
                     /locus_tag="PK977_11840"
     CDS             complement(<1116..1312)
                     /locus_tag="PK977_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF18855.1"
                     /translation="MNKHIPLAERVRPVTLDELLGQQHLVGKGSILRTSIENSKIPSM
                     IFWGPPGTGKTTIANIIAHTL"
     assembly_gap    1468..1482
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1483..2240)
                     /locus_tag="PK977_11845"
     CDS             complement(<1483..2240)
                     /locus_tag="PK977_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF18856.1"
                     /translation="MKHWPLIVFSALILASCQKDNDSAPDPPLPEKTELNVAYGADAA
                     QKMDVYLPAGRSTNTKVMIMIHGGGWTQGDKSEFTAYVDTLKRRLPGYAIFNINYRLA
                     TGSVNFFPTQENDVKAALDFIYSKRNEYSISDKYVLLGASAGGHLSLLQAYKYTSPVK
                     IKAVVDFFGPTELVNMYNNPPNPLIPLLLLQVTGGNPSTHAALYQQSSPLNFVTSQSP
                     PTIILHGGVDIVVAPSQSVLLRNQLQALGVVHQYV"
     gene            2420..3808
                     /locus_tag="PK977_11850"
     CDS             2420..3808
                     /locus_tag="PK977_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18857.1"
                     /translation="MKRSFVFIAVIFSVLQVCAQDKYALLFSRPVRTNETKQDEFNKQ
                     INRNNIRVISNGLSIQRFNPSKIFKDTAGNIRQNILKAVTDLTLRIRQGDFVFLYFDV
                     PVFINKTTQEAGFGYSEDDFVSFQEMNELFKKIGTKNNDPSLFFVLVDADIPKETKIG
                     SRLYSHYLFASQPGQFGALSDNTTVFALAVEKAMKSISGLNASYEGFWGSVKSNILFF
                     TTKQTPLLLSPDMATGFFNNLSLKFPRYYQIVEKKSDNTVIINAGIRMNIAKGLKVQF
                     YPPYSDTSGQKPFVTGTVLDVSSATANVSLSRPVKEEVNSYWAFIPGYDFSMLTPVIR
                     FNKKANGIDNSSKEIFKQVLAECKKDQNNSFIQLTDQAGDIGVGSIISLPGKRYEITL
                     LNPQTSQVLTTMVINGIEELHKVSGFCKKLAQYEYISNLTNYIPELAVDFQMTDKQGN
                     PLTAKENGYDIL"
     gene            3795..5603
                     /locus_tag="PK977_11855"
     CDS             3795..5603
                     /locus_tag="PK977_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF18858.1"
                     /translation="MIFYEGDEVIIKIRNTNPHKLYYSLVDLTQDKNFTVISGRDMED
                     HFILPNSELKLPIMISAPFGKERIKLFTSNVPFDLGDFQNATTRSVKNNVGFADINIQ
                     DYDFESRSSLYARNAKQNGELLSIMVKDLPKANSRTIAIRNPSSERIYFNVYLQKEDG
                     GYTTIFPGKGLKDKDCYVDFGVTRDFSFDEQLKAFDQLITVYADRPFDLEGLISGERK
                     LNGLFAEIAKNGRIRGTALNKIGLTQALFQPGTTTTMRDGETIFIKLITPKVSNDRGI
                     TIPTPSQQYEINGFAMSVDNKPVRSLKINNEPVNYDANLKFFEHTVNLSFGVNKVIIE
                     ATDEKGFTAVRTLDFELKNNNAVMAGGKGKNYFLGIGIDNYKTWPVLNNARNDVVKFA
                     ELLRSKYGFDSSFLLLDEQATRKNIINKIREFLKKAGPNDNVVIYLSGHGNEDQLADG
                     DYYFIPQEAEADDVTGAVKSTDIVDNFKKIRAKRCLLIVDACYSGMITNSVNPSGIPL
                     TSGTDLESAENAPCKWIVTSGRATKVSDGEKGKNSPFATVLINYLREHEDDASLKMPR
                     LIDFLKEKVKELSKQQEPFGMSIEGRGEWIFRIPGK"
     assembly_gap    5946..5955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5956..6250
                     /locus_tag="PK977_11860"
     CDS             <5956..6250
                     /locus_tag="PK977_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18859.1"
                     /translation="KAVNQKNNPVQEDKSRGNVYGPDYSDVVVDNWTGYYIDIYVNGN
                     YRGTVSPWDKRVTWAIPGTNTLYAKAVFDDGSYLYWGPKVTYTGYQYTWKLNP"
     assembly_gap    6713..6748
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6749..>7072)
                     /locus_tag="PK977_11865"
     CDS             complement(<6749..>7072)
                     /locus_tag="PK977_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18860.1"
                     /translation="IAVIRKFDPSNENYALKDLDFKLALYVDKDPAKATSLLISYLKD
                     NQKPVSVEAIGNWFINYASRFEEENFKTMSTELDLFKLRAIDKDKEYWLYLMQMLCSA
                     QAGNEV"
BASE COUNT         2129 a         1287 c         1472 g         2078 t
ORIGIN      
        1 gccagttttt tcctttgtta ttcgatccgg ctgtaataag tcaataattg tacttgtctt
       61 tccagagatt ttttaaaaac ttacgcaatt cttcttctct tgcatttttg ccgggttcat
      121 agaatttggt gccggctaat tctttgggta aatattcctg gggagagaaa ttgttctcat
      181 agctatggga gtattcataa ttttttccat agtctaaatt tttcattaat gcagtaggcg
      241 cattgcggat atgcagcggc acagaaaggt cgcctctttt tcttacttct gcaagtgctg
      301 cgtctatggc tgcataagaa gcattactct ttggtgtgga ggccaggtaa atggcacatt
      361 gagaaagaat gatccttgct tccgggttac caattttatt gacagcatca aacgtagcat
      421 tggccatcac taatgcggtt gggttagcat tgccaatatc ctcactggcc agtatcacca
      481 ttctccgggc aatgaatttt acatcttcac caccttctat catgcgggcc aaccagtaca
      541 ccgcagcatt gggatcgctg cccctgatgg atttgatgaa agcagagata atatcataat
      601 gctgttcgcc ggttttatca tacaaagcaa ctcttctttg cgctacctcc attacaaact
      661 tgtcagtaat aacaactttt tcaccggagg aatcacagac tatttccagc aggtttaaaa
      721 gttttctgcc atcaccaccg gagatattga taagtgcagc cgtttcgttt aattcaactt
      781 ttttcttttg gagaaactca tctttctcaa tagcggtatg catcaatttc accaggtcaa
      841 tttcctccag gggctttaat acatatacct ggcaacggct tagcaaggca gaattgactt
      901 cgaacgaagg gttttctgtg gtagcaccaa tcaaagtgat agtgcctttt tctactgccc
      961 ccagcaaggc atcctgctgg cttttattga agcgatgaat ctcatcaata aataaaacag
     1021 atccggattg tgatttagcg gtttcaatga cttccctgat gtcttttaca nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaag tgtatgagca attatattgg
     1141 caatagttgt tttgcctgta cccggcggcc cccagaaaat catggagggt attttactat
     1201 tttcaataga tgttcgtagg atactgcctt tgccaacaag gtgttgctgc ccgagtaatt
     1261 catccagtgt taccggtcgt acccgttctg ccaatggtat atgcttattc acccttcaaa
     1321 gtaacaaaaa agcattcctt gatggggaat gctttcaata attatttgta caggtcagtt
     1381 tacattggcc gtaataaagg cctgtatctt atcaaatgaa tcggccaggt tggtaccggt
     1441 ccaaccatgg ttttcagttg gataaaannn nnnnnnnnnn nnacatattg atgaaccaca
     1501 cccaatgcct gtaactggtt tcgaagtaac acggattgag aaggggctac aactatatcc
     1561 acaccgccgt gtaatataat ggtaggaggg ctttgcgacg tgacgaaatt taaagggctt
     1621 gattgctgat acaatgcagc atgggtactt ggattaccac cggtcacctg tagcagcagt
     1681 aaggggatca gcgggttggg cggattatta tacatattca ccagctcagt tgggccgaaa
     1741 aagtcaacaa ccgctttaat ttttaccggt gatgtatatt tataagcttg cagcaatgac
     1801 aggtgtccgc cggcactggc tccgagcaaa acatatttat cgctgatgga atattcattt
     1861 cttttactat aaataaaatc caatgcagct tttacatcat tttcctgggt gggaaagaaa
     1921 ttaaccgatc cggtagccag ccggtagtta atattgaata tggcataacc gggcagtctt
     1981 cttttcagcg tatctacata cgcagtaaac tctgatttat ctccctgtgt ccatccgccg
     2041 ccatggatca tgatcatgac tttggtgttg gtacttctgc cagccggtag atatacatcc
     2101 attttttgag ccgcatcagc gccatatgct acatttagct ctgttttttc aggtaatggc
     2161 ggatcaggcg ccgagtcatt atctttttga caggaggcaa gtatcaaagc actaaaaacg
     2221 atcagtggcc aatgcttcat acagtgattt tttagtaagt taaagaaaag ttggcgcttt
     2281 tattgccgga aacagattca gtatataccg gctatgatgt gtcaatttta cccggagttg
     2341 cttgagaagc aggcttattt gaagaaaaat accttttggc cacaggctta tgtaatcagt
     2401 aagaggttaa attcggggta tgaaaaggtc ttttgtcttt atagctgtaa ttttttctgt
     2461 cttacaggta tgtgcacagg ataaatatgc tttattgttt tcaaggccgg tcagaacaaa
     2521 cgagaccaaa caggatgaat tcaataagca gatcaatcgt aataacatca gggtaattag
     2581 taatgggctt tccatacaac gatttaaccc atccaaaata ttcaaagata cggctggcaa
     2641 tatccgccaa aacattttga aagctgttac agatcttact ttaagaatac ggcaaggaga
     2701 ttttgtgttt ttgtattttg atgttccggt ttttatcaat aaaactactc aagaggcagg
     2761 ttttggttat tcggaagatg attttgtttc attccaggaa atgaatgagt tatttaaaaa
     2821 aatcggtact aagaataatg atccttcatt gttttttgtt ttggtggatg cagatattcc
     2881 aaaagaaact aaaattggca gtagacttta cagtcattat ttgtttgcca gtcagcctgg
     2941 tcagttcggg gctttgagcg ataacactac agtatttgct ttagcagtcg agaaagcaat
     3001 gaaatcaatt tccggtctga atgcctctta tgagggtttt tggggcagtg taaaatcaaa
     3061 tattttattt tttaccacga agcaaacgcc tttattatta tcgcccgata tggcaacagg
     3121 cttttttaat aatctgtcat tgaagtttcc caggtattat caaatcgttg aaaaaaagag
     3181 tgataataca gtcatcataa atgcgggaat cagaatgaat attgccaagg gtttgaaagt
     3241 acagttttat ccgccttact cagatacatc cggtcaaaag ccatttgtaa caggtacagt
     3301 attagatgtt tcatcagcta ctgcgaatgt aagtttaagc aggccggtaa aggaagaagt
     3361 aaatagttac tgggcgttta tccccggtta tgatttttca atgcttaccc ctgtaatcag
     3421 gttcaataaa aaggccaatg gtattgataa cagttcgaaa gaaatattta aacaagtatt
     3481 ggccgaatgc aagaaagacc agaacaactc atttatccaa ctaacagatc aggctggcga
     3541 tatcggggtt ggaagtatca tcagtctgcc cggaaagaga tatgaaataa cattattaaa
     3601 tccacagacc agccaggtgc tcactacaat ggtaattaac ggaatagagg agttgcataa
     3661 ggtatccggc ttttgcaaaa agctggcaca atatgagtat atatcaaacc ttactaatta
     3721 tatacctgaa ctggcagttg attttcagat gacagataaa cagggaaatc ccctgacggc
     3781 gaaggaaaat ggatatgata ttctatgaag gggatgaggt tatcattaaa atccgtaaca
     3841 caaatccaca caaactttat tatagcctgg tggatcttac ccaggataaa aattttaccg
     3901 ttatatcggg aagagatatg gaagatcatt ttattttgcc aaactccgag ctaaaacttc
     3961 ccattatgat cagtgcccct tttggaaagg agaggataaa attgttcaca agcaatgttc
     4021 cttttgacct tggtgatttt caaaatgcta ctacccgttc agtaaagaat aatgttggtt
     4081 ttgcagatat taacatacag gattatgatt ttgaatcaag aagttctttg tatgcgagga
     4141 atgcaaaaca aaatggtgag ttgctttcta ttatggttaa ggatttacca aaagcaaata
     4201 gcaggacaat agctatcagg aacccaagtt ccgaaaggat atattttaat gtgtacctgc
     4261 agaaagagga tggaggctat acaacgattt ttcccggcaa aggattaaag gataaggatt
     4321 gctatgtgga ttttggagtt acccgggatt tctcatttga tgagcaattg aaggcttttg
     4381 accagttaat tacagtgtat gctgaccggc cttttgatct tgaggggcta atttccggtg
     4441 aaaggaaact caatggcctt tttgctgaga ttgccaaaaa tggccgcatt cgcggaacgg
     4501 cattgaataa aattggatta acgcaagcat tgtttcagcc cggaactact acaaccatga
     4561 gagatggtga aactatattt attaaactca taacccctaa agtctccaat gacaggggaa
     4621 taaccattcc gacaccctct cagcagtacg aaataaacgg gtttgcaatg tccgtggata
     4681 ataaaccggt aaggtcctta aagatcaata atgaaccggt taattatgat gccaatctca
     4741 aattttttga acatacggtt aatctttctt ttggtgtaaa caaagtaata attgaggcta
     4801 ccgacgagaa agggtttacg gccgtccgta ctcttgattt tgaactaaaa aataacaatg
     4861 ctgtaatggc tggaggtaag ggtaagaact attttcttgg aattggcatt gataattata
     4921 aaacatggcc cgtacttaat aatgccagaa acgatgtggt taagtttgct gagctgctga
     4981 ggtcaaaata tggttttgac agcagtttcc tgctactgga tgagcaggcc acccggaaaa
     5041 atatcattaa taagatcagg gagtttttga agaaagcagg acccaatgat aatgtggtca
     5101 tttatttatc tggtcatggt aacgaagacc agctggctga tggtgattat tattttattc
     5161 cgcaggaagc cgaagcagat gatgttacag gagcagtgaa atcaaccgat atagtggata
     5221 atttcaaaaa gataagggca aagcgttgcc tgctgattgt ggatgcctgt tactcgggga
     5281 tgataactaa tagtgtaaac ccttccggta ttccccttac ttccggaaca gaccttgaat
     5341 cagccgaaaa tgcaccgtgt aaatggatag tgacatcggg cagggccacc aaagtttctg
     5401 atggggagaa gggcaagaac agcccttttg ccaccgtgct gatcaactac ctcagggaac
     5461 atgaggatga tgccagcctt aaaatgccga ggctaataga ctttctcaaa gaaaaggtga
     5521 aagaactgag taaacagcag gaaccctttg gtatgtcaat tgagggccgg ggagagtgga
     5581 tattcaggat accaggtaag tagcatacgc ttaatattgt ttctttgaaa atacccgctt
     5641 aacggtagtt gaaacaggta attacagtag tatcttagta acatcaaatt atcggtgtac
     5701 aggataatat aaattaagat attttttaaa gtccatgcat ccttaagcaa aacttttgta
     5761 tcattgattt ctaaaacaaa aaacattaaa actcacacat tatgaaaaag catcttcttc
     5821 ttttagccac cttttttgtt gttggctttg gaatcacggc caatgctcag gtaagaggta
     5881 agtacaaaaa agaaacaatt gtatccaatg ccatttctaa ccccgatcca aaagtggacg
     5941 gattgnnnnn nnnnnaaagg cggtaaacca aaaaaacaac ccggtacagg aagacaaatc
     6001 caggggtaat gtttacgggc ctgattatag cgatgtagta gttgacaact ggactggtta
     6061 ttacatcgac atctatgtaa atggtaacta tcgtggtact gtttctccgt gggataaaag
     6121 agtaacatgg gcaattccag gtactaacac tttgtatgca aaagcagttt ttgatgatgg
     6181 aagctacctt tactggggtc ccaaagtaac ttacaccggt taccagtaca catggaaact
     6241 gaatccataa tctctatttc taaataagca atatttcgaa ggtcgcattt atgcggcctt
     6301 tttattgtag ataccagtcg aggataaaag aataagaaat tgtagcatgc tactttcaaa
     6361 atgtaaaaga atctctgacc atggtaaggg tacgaattaa tatcaaaacc gccagataga
     6421 taataagcta aatcatgcga gggatagcat aagattttac gatcagatca gcaggacaga
     6481 caaagcttct caaagagaat aatactggtc gtaaagcccg atacattgac ttaaccaaag
     6541 tttagttatg attgcccggg tgatatcagg gagcaactaa aaagccgcaa tagtgatgta
     6601 actattgcgg ctttttaaac aacttaatag cgtttaatcg gtaagactat aggtttcttt
     6661 taaataggat ttgtattctg caggaataag tggatgttta taagccttta tannnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta cttcgttacc agcctgagca gaacaaagca
     6781 tttgcatcag gtatagccaa tattccttgt ctttgtcaat agctcttaat ttgaacagat
     6841 ccagttctgt actcatggtt ttaaaatttt cttcttcgaa tcggctggcg tagtttataa
     6901 accagttgcc aatagcttcc acacttacgg gtttttgatt gtctttgagg tagctgatca
     6961 gaaggctggt ggcttttgcc ggatccttat caacataaag agccagttta aaatcgaggt
     7021 ctttaagagc atagttttca ttcgaaggat caaatttcct gattactgca at
//
LOCUS       DAORLS010000154         7052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5769_length_7052_cov_2.023724, whole genome shotgun sequence.
ACCESSION   DAORLS010000154 DAORLS010000000
VERSION     DAORLS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5769_length_7052_cov_2.023724"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>914
                     /locus_tag="PK977_11870"
     CDS             39..>914
                     /locus_tag="PK977_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18861.1"
                     /translation="MKRIFLSTFVGLLFCFTHCSGQDLAAILNSGKTAAEKAELITAQ
                     ARKFFQTMKWNEAAQWQEEGIKLAVTTGIDTLVGKLYLDRGNLAIMQGMPKQALDYLS
                     KGNTHLGRTTAWYHMGSCLLLTAKSYARLEDTDSALFYFRRAEETYNKVNPYNNWATY
                     IEMGTFFGSAENHDEAKKYFEKAYQLTKQGTNRINHGLAMYNLASFYYKRNMTAEFAR
                     LYTEQQEYYNTSKKDFSKDPVHSFLKMDWGDKTLEQKVAFLLSVKKTLLAQNLVTNGS
                     SINEEIALLYEKENRF"
     assembly_gap    915..1027
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1028..2165
                     /locus_tag="PK977_11875"
     CDS             <1028..2165
                     /locus_tag="PK977_11875"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRF18862.1"
                     /translation="EAIAMADRMIALKDSVTRMQNLQLAMELDARYLSEKKGKEIELL
                     NANNEIARKEIALLNSQKKLTDIELLHQFELQKALARENDLMDSVVAQEKRNNELLSR
                     ENFLKQSELQKETALKASLDRENKLKAVQLEKERNTRTGLISAAGLLTLAVGVILFQY
                     RKQRLKNKVIQKQSDDMQVLMKEIHHRVKNNLQVISSLLDLQSMTIADNQASEAVKEG
                     KNRVQSMALIHQNLYSEGNIKGIRTKEYINNLLQSLCDSYNVTNDKVKVKTDIDDLNL
                     DVDTMIPLGLVLNELVSNTLKYAFRNGTKGELNVVLRKEPEHLLLRVSDNGAGFPEGV
                     NIKEGKSFGMKMIRAFAQKLKANLSIYNNNGAVTEMQITKYNLA"
     gene            2162..2785
                     /locus_tag="PK977_11880"
     CDS             2162..2785
                     /locus_tag="PK977_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF18863.1"
                     /translation="MSKLRILIVEDEPVIAENISMYLDNADFEVSGIAYDSLEAKDQL
                     ITNTPDAAILDVNLESEEDGIDIAGYINEKFQLPFLFLTSYSDKETLERAKKVKPSGY
                     IVKPFNEKTLLASLEIAISNFASEKNHSLPPLSLDKINSFLLSPISDREFELLQLLYE
                     GITNTQIAEKLFISINTVKTHLKSIYLKLDAGTRIDVIKKLRGIMLK"
     gene            2911..3684
                     /locus_tag="PK977_11885"
     CDS             2911..3684
                     /locus_tag="PK977_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18864.1"
                     /translation="MKRVLFLFLLFAAAICSFAQVATVNFSGSTKITPGTSNRITVSV
                     KNTQKLDIVKTRTPWLVTMIYKGSTQAGQAFNKTVDLGPSLGAGQTKNYVFTVEGPTL
                     PGEYDVDIVLKRGSDVESNVEKVTFVVAASYEVSIVPKVTSYSIDRGRTQWIALKFTL
                     VNTGNTAWPEGKYSLDFTSLSSPGGASATDKKVFDNDPKEVEFWDLEPNGSAEFDIPK
                     FTPPYSGGAYSFKVTLLLNGRPFDADGNPKTVTLNFNVR"
     gene            3700..4245
                     /locus_tag="PK977_11890"
     CDS             3700..4245
                     /locus_tag="PK977_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18865.1"
                     /translation="MKKVLFPILVILLAVSHVNAQWPSNVTSIESKANDSVIVSGDLA
                     SGALMEDLSWAANSSNACFPATQNLKFRGNHVLYATSIPPRSILTISVNPADANGDLS
                     IYGYMMGNTEYRVVPNLPSCISCEADHKWDGKWKGKVQTSERKIEFQNPTQNTYNIVI
                     GVAAPKDVITGKFNLKLKLKS"
     gene            4270..4776
                     /locus_tag="PK977_11895"
     CDS             4270..4776
                     /locus_tag="PK977_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18866.1"
                     /translation="MKYLLFLSSLFLAINTFSQGNTGSRKKSSQVATSANAPKTRKKA
                     SGNEVAMEELHLNKPTSSKEQARPEAPVSQKTKLDDLKDPFDSTKKLKGNQPATVQSS
                     SHDRYANIETNYKQDNKQVQQSTASQNVEKTKLDDLKNPFDSTKKGNPGVQSGQVPAS
                     QKGRANRQ"
     gene            4996..5376
                     /locus_tag="PK977_11900"
     CDS             4996..5376
                     /locus_tag="PK977_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18867.1"
                     /translation="MVQVDISQLSSECNVWREKLRMYREELNNDETKLRQVAGKELTK
                     EQLQDVEHLHNQFHIQLINIHDLKQAIKAHDRRMNFEKVAFNGHVNEDSFSRHESLFS
                     EYQVLEQTLQDLRQEFAGFLERSK"
     gene            5503..>6278
                     /locus_tag="PK977_11905"
     CDS             5503..>6278
                     /locus_tag="PK977_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF18868.1"
                     /translation="MPEFDTSHVKNIVLLGHAGSGKTTLAECMLYEAGIITRRGSIAE
                     RNTTGDYHELEQERGNSIFSKLLHTKWRGYKINIIDTPGYDDFVGEVISALRVADTGV
                     MLLNAVMGVEVGTDIIWEYTEQFKTPMIFAVNKLDDDNADFDKTIREAKSHFGSKVVM
                     VQYPRQIGAGFHEVIDVLNMVMYKFKEGGGKPDKLPIPDDEKSRADELHKELIEAIAS
                     NDEGLMEKYFDKGELDEDEMKVGLKKAMINHDLFPVFCISA"
     assembly_gap    6279..6311
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6312..>6673
                     /locus_tag="PK977_11910"
     CDS             <6312..>6673
                     /locus_tag="PK977_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRF18869.1"
                     /translation="GRLMGYIDNVCPSANEMPPQKTKRGEDLPCDANGPACIFIYKTI
                     SEPHVGELSFFKVYSGTIKVGMELENETTGVTEKINQLYLVEGNKRTSTNELVAGDIG
                     STLKLKNSHVNNTLHAKG"
     assembly_gap    6674..6734
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6735..>7052
                     /locus_tag="PK977_11915"
     CDS             <6735..>7052
                     /locus_tag="PK977_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRF18870.1"
                     /translation="ETLKKGEEEKLSQALHQLKEEDPTLLVEVSSELKQTLIHCQGDM
                     HLAVAKWKIEHNFKLEVKFVKPKIAYRETIRKAADSSYRHKKQSGGAGQFGEVFMRIE
                     PWY"
BASE COUNT         2287 a         1289 c         1511 g         1758 t
ORIGIN      
        1 tttttaaatt acgatataaa cactattgcc ccgactgcat gaaacggata ttcttatcga
       61 cttttgtagg cttacttttc tgttttactc attgcagtgg ccaggatctg gctgctattt
      121 taaacagtgg taaaactgct gctgaaaaag cggagcttat aactgcccag gcccgtaagt
      181 tcttccaaac catgaaatgg aatgaagcag cgcaatggca ggaagaagga attaaacttg
      241 cagtcacaac cggcattgat acccttgttg gtaaactcta tttagacaga ggtaatctcg
      301 ccatcatgca gggtatgccg aagcaggctc ttgattattt atccaaaggg aacacacatc
      361 ttggcagaac taccgcctgg tatcatatgg gtagttgttt attgttgacc gcaaaaagtt
      421 atgcaagact tgaggatact gattcagccc ttttttattt cagaagggca gaagagactt
      481 ataacaaagt caatccttat aataattggg ctacttatat cgaaatggga actttttttg
      541 gttctgccga aaatcatgat gaagcaaaga agtattttga aaaagcttac cagttgacaa
      601 agcagggaac taacagaata aatcatggat tagccatgta taaccttgcc agtttttatt
      661 acaaacgtaa tatgactgct gagtttgccc gtctttatac tgagcagcag gagtattaca
      721 ataccagtaa aaaagatttt tccaaagatc cagtacatag ttttctaaaa atggattggg
      781 gcgataaaac gttggaacag aaagtggctt tcctcttgtc ggttaaaaaa actctgctgg
      841 cacaaaacct ggtcaccaac ggaagtagca taaatgagga aattgccctc ctttatgaaa
      901 aggaaaaccg gtttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnaga agctattgcc atggctgaca gaatgattgc attgaaggac agtgtaaccc
     1081 gtatgcaaaa ccttcaactg gcaatggagc tggatgcccg ttacctttct gagaaaaagg
     1141 gaaaggaaat tgaattgctg aatgctaata atgaaattgc ccgcaaagag atagccttgc
     1201 tgaattcaca gaagaaactt actgatatag aattattgca ccagtttgaa ctgcagaaag
     1261 cactagcaag ggaaaacgac ctgatggatt cggtagtagc gcaggaaaaa agaaataatg
     1321 aattattgtc tagagaaaat tttttgaaac aatcagaact gcaaaaggaa acagccctga
     1381 aagcatcgct tgaccgggaa aataaactca aggctgttca gttagagaaa gaaaggaata
     1441 cccgtacagg acttatttca gcagccggct tactgacatt agcggtaggt gtcatattat
     1501 ttcaataccg gaaacaacgg ctgaagaata aggtcatcca gaagcagtcg gatgatatgc
     1561 aggtgctgat gaaggagatt catcatcggg taaaaaacaa cctgcaggta atcagcagcc
     1621 tgctggattt acaaagcatg accattgccg ataaccaggc cagtgaggcg gtaaaagaag
     1681 gaaaaaaccg ggtgcagagc atggcattga ttcatcaaaa cctgtacagc gagggaaata
     1741 ttaaaggtat caggacaaag gagtatatca ataacctgct gcaaagtctc tgcgactctt
     1801 ataatgtcac taacgataag gtgaaagtaa aaactgatat agatgacctg aacctggatg
     1861 tggataccat gataccgctt ggactggtat tgaacgaact ggtaagcaat acgcttaaat
     1921 atgcattcag aaatggtacg aagggagaat taaatgtagt actcaggaaa gagccggaac
     1981 acctgttgct gcgggtaagt gataatggtg ccggattccc cgaaggagtc aatataaaag
     2041 aagggaaatc gtttggtatg aaaatgattc gggcatttgc gcagaaacta aaagccaacc
     2101 tgagtattta taataacaat ggtgcagtaa cggaaatgca gatcaccaaa tacaacctgg
     2161 catgagtaaa cttcgaatcc tgatagtgga agatgagccg gttattgcgg agaatatttc
     2221 tatgtacctc gataatgcgg attttgaggt gagcggtatt gcctacgaca gtttggaagc
     2281 aaaagatcag ttaataacca atacacctga tgcagcaatt ctcgatgtaa acctggaaag
     2341 cgaggaagat ggtattgaca ttgcaggcta cattaatgag aaatttcaat tgccttttct
     2401 ttttctgact tcttattctg ataaagaaac actggaacgg gcaaagaaag taaagcccag
     2461 cggatatatc gttaagcctt ttaatgaaaa aacattactg gcatcacttg aaattgccat
     2521 ttccaatttt gcatctgaaa aaaatcattc tttgcctccc ctaagccttg acaaaatcaa
     2581 cagttttttg ctgtcaccta taagtgacag ggagtttgaa ctgctacagc ttctctatga
     2641 gggtataaca aacacccaga ttgcagaaaa acttttcatt tctatcaata ctgtcaagac
     2701 ccacctgaaa agtatctatt tgaaattaga tgcaggaact cgtattgacg tgattaaaaa
     2761 acttcggggc attatgctga aataaaatca cccaaagggg tgagggctta ggatatctgc
     2821 tatctaattt tatacagcca ttgcaacgta taatttttta aagaaacctt tcacctatag
     2881 tcaaaaacct taatcattaa acttctctgt atgaaaagag tattatttct ttttttactg
     2941 tttgcggcag caatttgttc attcgcccag gtggcaactg taaatttttc cggcagtacc
     3001 aaaataacgc ctggcaccag taatcgtatt acagtttcgg taaaaaatac acagaaactt
     3061 gatattgtca agacaagaac tccctggctg gttactatga tctataaagg ctcgacgcag
     3121 gctggtcagg cttttaataa aacggttgac ctggggccat cattaggagc cggacaaact
     3181 aaaaattatg tttttacagt ggaaggcccc acattgccag gagaatatga tgtagatatt
     3241 gttttgaaaa gaggaagcga tgtggaaagt aatgttgaaa aagtaacatt tgttgttgct
     3301 gccagttatg aagtgtcaat agtgcctaag gttaccagct atagtatcga caggggtcgt
     3361 acacaatgga tcgcattgaa atttacacta gttaatacag gcaatacggc ctggccggaa
     3421 ggaaagtaca gccttgattt tacttcacta tcttctcccg gcggggcaag tgctacagac
     3481 aagaaagtat ttgataacga tccaaaagaa gttgaattct gggacctgga accaaatgga
     3541 agtgcagaat tcgatattcc aaaatttact cctccatatt caggaggggc ctattctttc
     3601 aaggtcactc ttttgctaaa tggaagacca tttgatgctg atggcaaccc aaaaacagtt
     3661 acgttaaact ttaatgtcag ataacctaaa acctccaata tgaaaaaagt acttttcccg
     3721 attttagtaa tcttactggc tgtatcacat gtaaatgcac aatggccatc caatgtaaca
     3781 agtattgaaa gcaaagcaaa tgactctgtc attgtatcag gcgacctggc aagcggagca
     3841 ttaatggaag acctcagctg ggcagccaac agttccaatg cttgtttccc ggctacacaa
     3901 aatcttaaat tcaggggcaa tcatgtgttg tatgccacca gtattccgcc aagatctatt
     3961 ctgacaattt cagtaaaccc tgcagatgcc aatggtgatc tgagtattta cgggtatatg
     4021 atggggaata cagaatacag ggtagttccg aaccttccca gttgcatttc ctgtgaggct
     4081 gaccataaat gggacgggaa atggaaagga aaagtgcaga ccagtgaaag aaaaattgag
     4141 tttcaaaacc ctactcagaa tacttacaat atagtaatag gtgtagctgc accaaaagat
     4201 gtaataacag gaaagtttaa cctgaaatta aaattaaaat cataataagc ttaaacaagc
     4261 ggaacggtca tgaaatattt attgttttta agcagtttgt ttctggcgat aaatactttt
     4321 tcgcaaggaa atacagggag ccggaaaaaa agcagccagg tggcaacatc tgccaatgca
     4381 cccaaaacaa gaaaaaaggc atcaggcaat gaagtggcaa tggaagaatt acatttgaat
     4441 aaaccgacgt cttcaaaaga acaggcacgg cccgaggcac cagtatctca aaaaacaaaa
     4501 ttggatgatt tgaaagatcc ttttgacagt actaaaaaat taaagggtaa tcagcctgca
     4561 acagtacaaa gtagttccca tgatcgttat gccaatatcg aaaccaacta taaacaagat
     4621 aacaaacagg tccaacagtc cactgcttcc caaaatgtgg aaaaaacaaa actggatgac
     4681 cttaagaacc catttgacag taccaaaaag gggaatccgg gtgtgcaatc cggccaggta
     4741 cctgcgagcc aaaagggaag ggcaaaccgg cagtagcggg gactgttacg aggatatcga
     4801 aaaatcaata gattacaaat tactaacaat gcacaggcag ctgcctgtgc atttcttttt
     4861 cgcaaaatat cctccacaaa tatttgaatt tttgtaaaat cgcattacct tactcatgag
     4921 catttaaccc accaggcaat ccgaaacccc acgaatccgt tagcatagat ttattcaatt
     4981 taaaatttta ttgccatggt tcaggtagac atctcacagc tctcttcaga atgcaatgtt
     5041 tggagagaaa aactccgcat gtacagggaa gaattgaaca acgatgaaac caaactccgc
     5101 caggttgccg gaaaagaact tacaaaagag caattacagg atgtagagca tttgcacaac
     5161 cagtttcaca ttcaactgat caatattcat gatctgaaac aggctatcaa ggctcatgac
     5221 cggagaatga actttgaaaa agtagctttc aacggacatg taaatgaaga tagtttttca
     5281 cggcatgaaa gcctgttttc agaataccag gtgctggaac aaaccttgca ggacctgaga
     5341 caggagtttg cgggttttct agaaaggtcg aaatagccat cctgaattat ctaccattga
     5401 ttttaatcag cttataacct aacagggttc atgagttggt attttattct ggtgaagatt
     5461 tgtgcttgtt ctttcatttt ctcacacata aaatttcttg ccatgcctga atttgatacc
     5521 tcacatgtaa aaaatattgt cttgctgggc catgccggtt ccggtaagac cacattagcg
     5581 gaatgtatgt tgtatgaggc cggcataatt acccgccgcg gcagcatagc cgagagaaac
     5641 actaccggag attaccacga actggaacag gaacgtggta attctatttt tagcaagctc
     5701 ctgcacacca aatggagggg gtataagatc aacattattg ataccccagg atatgatgac
     5761 tttgtaggcg aggttatttc tgcccttaga gtagcggata cgggagtgat gctgctcaat
     5821 gccgtgatgg gtgtggaagt gggtacagat attatctggg aatatacgga gcagttcaaa
     5881 accccgatga tttttgccgt gaacaagctg gatgatgata atgcagattt tgataaaacg
     5941 ataagggagg ccaaatctca ttttggcagt aaagtagtca tggtgcaata tccccgccag
     6001 attggtgcag ggttccatga agtgatcgat gtactgaata tggtgatgta taaattcaaa
     6061 gagggtggtg gcaagccgga taaattgccg attccggatg atgaaaaatc aagagctgat
     6121 gagctgcata aggaactgat agaggccatt gcaagtaacg atgaaggcct gatggaaaaa
     6181 tattttgata aaggcgaact ggatgaggat gaaatgaaag tgggcttgaa aaaagccatg
     6241 atcaatcatg acctttttcc tgttttctgt atttcggcnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nggccgcttg atgggttata ttgacaacgt atgccccagc gccaatgaaa
     6361 tgcctccgca gaaaacgaaa aggggagaag accttccttg cgacgccaat gggcctgcct
     6421 gtatttttat ttataaaacc atttccgagc cgcatgttgg cgaactgtct ttctttaaag
     6481 tttattccgg aacgatcaaa gtaggaatgg agctggaaaa tgagacgacc ggggttacgg
     6541 aaaagatcaa ccagctttat ttggttgagg gcaataagcg tacgtccacc aatgaactgg
     6601 tggccggaga tattggctca acattgaaat tgaagaattc ccatgtaaat aataccctgc
     6661 atgcaaaggg taannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnntgaaac actgaaaaaa ggagaggagg aaaagttatc tcaggcattg
     6781 caccagctga aagaagaaga tcccactctg ctggtagaag tttcatcaga gttaaaacaa
     6841 acgctaattc attgccaggg cgacatgcac cttgctgttg ccaaatggaa gatcgagcat
     6901 aatttcaaac tggaagtgaa atttgtgaaa ccaaaaattg cctacagaga aacaatccgc
     6961 aaagcagcag attccagtta ccgccataaa aaacaaagtg gtggtgccgg ccagtttggt
     7021 gaagtattca tgcggataga accctggtat ga
//
LOCUS       DAORLS010000155         7043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5783_length_7043_cov_2.242988, whole genome shotgun sequence.
ACCESSION   DAORLS010000155 DAORLS010000000
VERSION     DAORLS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5783_length_7043_cov_2.242988"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2070)
                     /gene="sprA"
                     /locus_tag="PK977_11920"
     CDS             complement(<1..>2070)
                     /gene="sprA"
                     /locus_tag="PK977_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF18871.1"
                     /translation="NYNTLANFNFENQLKLDFRGKDDDIVKIFQAGNTNFASKGTLIP
                     GAQSLFGVKTQLQFGKLFVTTVLANQQSQRQTQSLQGGSSTQSFSLKADEYDENRHFL
                     MGQYFRNNYNTAMKQLPVVNSKVQILRVEVWVTNRNGTTTDTRDVVAFMDLGENNPYN
                     SGVITGTGDPLPKNEANNLYSTLRSNPNYRNSSTVQNQLTTLGLLPVQDFEKTFARKL
                     QPTDYYFNDRIGFISLNQPLQPDEVLGIAFQYTYNGRVYQVGEFSQDVPPDTTGTSQR
                     VLFLKLLKATSQRTNLPIWDLMMKNVYSVGFGQLERQDFRLDLTYEEPSLGEKRYLPP
                     TDVINTYEGAPIISLVNLDRLNNQNDPQPDGVFDFVEGFTVISSQSRIVFPVLEPFGH
                     DLDYIYANQTLSNKYLYYPLYDTIKAIAQTFANLNRFKIVGRSKSSGVAPGFGGNSFA
                     GSANNPASGEYQLGFNIPRGSVTVTANGQVLQENVDYEINYDLGTLRVTNSAILASGV
                     PVNIAYENNQAFGFQQKNFLGVRFDYLASKKLSLGGTIVRLGERPFFIKQTYGEDPIR
                     NTMYGIDVDYRNNLPRLTKWLDKLPFYSTKSMSSITAYAEAALLDPGHAKQINPSRGD
                     RGGQVYIDDFEGTRANIDLRFPIISWTLASVPQGNGLFPESSLMNDLRSGYNRAKIAW
                     YNIEPVLQ"
     assembly_gap    2071..2207
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2208..2728)
                     /locus_tag="PK977_11925"
     CDS             complement(<2208..2728)
                     /locus_tag="PK977_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18872.1"
                     /translation="MNLIAAHIFRTLVVIAFVLVCNLPAFAYPGGITNNNNTGFFSDT
                     TRYPLLDRYGDPYSNPNRNTFDFRDTSFIKRTVEYDPRTNKYYIVEKIGNQYYRTPTS
                     FTMEEFVRLKGRQDEQEYFKKRSALLSNMNRRLFKPKFKPSGDWFNRIMGAGPDGKVK
                     IDIRPTGYVDFMAG"
     gene            complement(2769..3350)
                     /gene="ruvA"
                     /locus_tag="PK977_11930"
     CDS             complement(2769..3350)
                     /gene="ruvA"
                     /locus_tag="PK977_11930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRF18873.1"
                     /translation="MLAFLKGNYVYKSPAVVHMDVNGVGYEVQISLNTYTKIQHLDEG
                     TLFTSLLIREDAHILFGFFDLAEKEMFLHLVSVSGIGASTARVMLSYMKPEELSRAII
                     NGDTRTLEGIKGIGKKTAERMVLELRDKLAKNPLESNIPALKNNTLQQDALNALTALG
                     ISRQAAGQALEKVLAANPALSVEELIKLALRTL"
     gene            3499..4500
                     /locus_tag="PK977_11935"
     CDS             3499..4500
                     /locus_tag="PK977_11935"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRF18874.1"
                     /translation="MPHSQKKITAAITAVGGFVPEDKLTNFDLEKMVDTNDEWIRTRT
                     GISERRILKGEGLATSDMVVPAVKQLCEKRGIHPEEIDCLIVGTVTPDHVFPATANIA
                     CDKLGAKNAWGFDISAACSGFLYSLTVGASLIESGRYKKVVVCGADKMSAIVDYQDRA
                     TCIIFGDGAGAVLLEPNHEGYGVLDSILKSDGSGCQFLHMKAGGSKKPASIETVMAKE
                     HYAYQEGQAVFKFAVKGMADVSAELLEKNNLTGEDIAWLVPHQANLRIIDATASRMGL
                     PKEKVMINIQKYGNTTAATIPLCLWEWRHQLNKGDNIVLAAFGGGFTWGATLVKWAY"
     assembly_gap    4663..4679
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4680..4939)
                     /locus_tag="PK977_11940"
     CDS             complement(<4680..4939)
                     /locus_tag="PK977_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18875.1"
                     /translation="MLSSVRSLLLSATIFVACSSCRDNRPHTIKDLDENMLDLKIYQE
                     NMGDELKAGRLQDAVWLLEGMDSIMLEVSRKFDEHRKLKDPFS"
     gene            complement(4948..6630)
                     /locus_tag="PK977_11945"
     CDS             complement(4948..6630)
                     /locus_tag="PK977_11945"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRF18876.1"
                     /translation="MPAVFPSDLEIAQSAKMKHIKEMAASIGVNADDIEYYGKYKAKL
                     PLKLIDKEKIAQHHLVLVTAITPTPYGEGKTTVSIGLTDGLNVIGKKAMAVLREPSLG
                     PVFGIKGGAAGGGYAQVVPMEDINLHFTGDFSAVEKANNLLSALIDNNLQNKTRNLHI
                     DPRTIVWKRVMDMNDRALRHILIGLGGINQGIPREDGFNITPASEIMAILCLSEDIDD
                     LKRKLGNIYIGMNDKGEPVFARDLNAVGAMAVLLKDAMKPNLVQTLENNPVIMHGGPF
                     ASIAQGTNTVNATKMGLSLADYVVTEAGFGADLGAEKFFDIKCAGAGLRPEAAVLVVT
                     IKALRHHGGAKKDELQTANIDKLLKGIPNMEKHIENLMKFGMKPVVAINYFPNDSQEE
                     IELVQQACKKYGVKAIVCKCFAEGGKGSTELAAEVVRIIENGESDFKPLYEADMTIEE
                     KMHKIATEIYGARGVEFSVKAKTHLKHIKELGFDKIPVCMAKTPKSLSDDETKRGRPE
                     NFVVTVREFEFASGAGFVIPILGEMMRMPGLPAIPAAENIDIDNNGKVTGLF"
     gene            complement(6708..>7043)
                     /locus_tag="PK977_11950"
     CDS             complement(6708..>7043)
                     /locus_tag="PK977_11950"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF18877.1"
                     /translation="SGWWQRIKMQERVIFPRDSFSGSMQDLFTGLINQSKQWEDWVQN
                     ASELSLRHVFHFHNSKKELFKQPVYQVLLHVFNHSTYHRGQLVTILRQLGIDKLPPLD
                     FIIWVRQQK"
BASE COUNT         1896 a         1523 c         1411 g         2059 t
ORIGIN      
        1 ctgcaatacc ggttcaatat tataccaggc aatctttgcc ctgttataac cactgcgcag
       61 atcattcatc agggatgatt cagggaataa tccatttccc tgcggcactg aagccagtgt
      121 ccagctaatg attggaaaac gaagatcaat attggcccgg gtaccttcaa agtcgtcaat
      181 atatacctgg ccaccacggt caccacggct gggattgatt tgtttggcat gaccgggatc
      241 cagcaatgca gcttcggcat atgctgtaat ggacgacatt gatttggtag aataaaacgg
      301 tagtttatcg agccactttg ttaatctggg taagttatta cgataatcca catcaatacc
      361 atacatcgta ttgcgaattg gatcttcacc ataagtctgc ttgatgaaga aaggcctttc
      421 tcctaatcgt actattgttc cgccgagtga aagtttttta ctggccagat aatcgaaacg
      481 tacgcccagg aaattctttt gctgaaagcc aaaggcctgg ttgttctcat aagcaatatt
      541 tacgggtaca cccgatgcga gaatggcaga atttgttacc cgcaatgtcc ctaggtcgta
      601 gttgatttca tagtcaacgt tttcctgtaa tacttgaccg ttggctgtta ccgtaacaga
      661 gccacgggga atattaaaac ctaactggta ctcacctgat gcagggttat tggcagaacc
      721 ggcaaaactg ttgccgccaa agcccggagc aacaccagat gatttggagc gacctacaat
      781 tttgaaacgg ttcagattgg caaaggtctg ggcaatcgct ttaatcgtat cataaagcgg
      841 atagtacaaa tatttgttac ttaatgtctg gttggcgtaa atataatcga ggtcgtgacc
      901 aaaaggttca agcaccggga atacaattcg gctttgcgat gaaataacag tgaagccctc
      961 tacaaagtca aacacaccat cgggctgcgg gtcattttga ttgttcagcc tgtcaagatt
     1021 caccaacgaa atgattggag ccccttcata tgtgttgatc acatcagttg gaggtaaata
     1081 tcttttttcg ccaaggctgg gttcttcata agtaagatcc aaacggaagt cctgtctttc
     1141 caattggccg aagcctactg aatagacatt cttcatcatc aggtcccaga taggaagatt
     1201 ggtacgctga gatgtagctt ttaataattt taagaataaa acccgttgtg aagtacctgt
     1261 tgtatctggc ggtacatcct gcgaaaattc acccacctgg tacactctgc cattataggt
     1321 atactgaaaa gctataccca atacttcatc aggctgcagt ggctggttta aagaaataaa
     1381 accgatacgg tcattgaaat aataatctgt aggctgcagt ttacgggcaa atgttttttc
     1441 aaaatcctgc accggcaaca agcccagggt agttaactgg ttttgaactg tggatgagtt
     1501 tcgataattg gggttgcttc ttagtgttga ataaagatta ttcgcttcat tttttggcaa
     1561 gggatctccg gttccggtaa tcacacctga attgtaagga ttgttttcac caagatccat
     1621 gaaggccacc acatccctgg tatcggtagt agtaccattt ctgttggtca cccaaacttc
     1681 tactctcagt atctgcactt ttgaatttac aaccggcagt tgcttcattg ccgtgttgta
     1741 attattgcgg aaatattgcc ccatcaggaa gtgcctgttc tcatcatatt catccgcttt
     1801 tagtgaaaag ctttgagtgg aagagccccc ctgtaagctt tgtgtttgcc tttgcgactg
     1861 ttggttggcc aacacagtgg ttacaaataa ttttccaaac tgcaactgtg tctttacacc
     1921 aaagagggat tgtgccccgg gaatcaaggt tcctttggaa gcgaagttgg tattacctgc
     1981 ctggaagatt tttacaatat catcatcttt tccacgaaaa tcgagtttga gttggttttc
     2041 aaaattgaaa ttggccagtg tattataatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc gccataaaat
     2221 caacataacc ggttggccgt atatcgattt ttactttacc atccggtccg gcacccatga
     2281 tacggttgaa ccaatcgcct gatggtttga attttggctt gaaaagccta cggttcatat
     2341 tggaaagtaa agccgatctt tttttgaagt attcttgctc atcctggcgt cccttcaatc
     2401 gcacaaactc ttccatggtg aaagaggtcg gagtacgata atactggttg ccgatttttt
     2461 ctactatgta atatttgttt gtcctgggat cgtattcaac cgttcttttg ataaaagaag
     2521 tatcccgaaa atcaaacgta ttcctgttgg ggttggaata agggtcaccg tatcggtcaa
     2581 gcaagggata acgggtggtg tcagaaaaga atccggtatt attattattt gtaatgcctc
     2641 cgggataagc aaaggcaggt aaattacata caagcacaaa ggcaataaca accaatgtac
     2701 ggaaaatatg agcagctata aggttcaatg ttttttctcc ttcagttagt ttcctgaaaa
     2761 ataatctgtt acagggtacg aagtgctaat ttaatcagtt cctcaacgga tagagccgga
     2821 ttggccgcca atactttttc cagggcttgc ccggcagctt gccggctgat gcccaaagcg
     2881 gttaaagcat ttaacgcatc ttgttgcaaa gtattgtttt tcagcgccgg aatatttgat
     2941 tccaacggat ttttagccag tttgtccctc agttccaata ccatccgttc ggctgttttt
     3001 ttaccaattc ctttgatgcc ttccagtgtc cgggtatcac cgtttattat agcccttgaa
     3061 agttcttcgg gtttcatgta ggacagcatt acccttgcag ttgatgctcc gataccagaa
     3121 acacttacca ggtgtaaaaa catctctttc tcggcgaggt caaaaaaacc aaaaaggata
     3181 tgtgcatctt ccctgatcag caggctggta aacagtgttc cctcatccaa atgctgaatt
     3241 ttggtataag tattcaggct tatctgcact tcatagccca caccattcac atccatatga
     3301 acaaccgccg gacttttata cacataattg cctttaagaa atgcaagcat aggttgcgaa
     3361 aataagggaa atgaaagagg agaaagcagt ttactaacaa ctgtttttat tccgcttctt
     3421 gtttattaat cagcagcgga atactggttc tatactcact gtttccatta actttgtcaa
     3481 cttataattt ttttaaatat gccacacagc caaaaaaaaa taacagcggc tattactgcc
     3541 gttggcggat ttgtgccgga agataaactg accaattttg atttggagaa aatggtagat
     3601 accaatgatg agtggatccg tacccgtacc ggaatttctg aaagaagaat tcttaaaggc
     3661 gaaggcttag ccacatcgga tatggtggta ccggcagtta aacaactttg tgagaaaaga
     3721 ggcatccacc cggaagaaat tgattgcctg atagtgggca ctgtaacacc tgatcatgta
     3781 ttcccggcta ctgctaatat tgcctgtgac aagctcggcg caaaaaatgc atggggtttt
     3841 gatatctctg cagcctgttc cgggtttctt tactcactaa cagttggagc ttcgctgatt
     3901 gaaagcggac ggtataaaaa agtggtagtg tgcggagcag ataaaatgag tgctattgtt
     3961 gattaccagg acagggctac ctgtatcatt ttcggcgatg gtgccggagc cgtgttactg
     4021 gaaccaaatc atgaaggcta tggtgtgctt gacagtattt taaaaagtga tggaagtggt
     4081 tgccagtttt tacacatgaa agccggcggt tctaaaaaac ctgccagtat tgaaacggtt
     4141 atggctaaag aacattatgc ttaccaggag ggacaggcag ttttcaaatt tgcagtaaaa
     4201 ggcatggccg atgtaagtgc agaattgctg gaaaaaaaca atcttaccgg cgaggatata
     4261 gcctggctgg taccgcacca ggccaatttg cggatcatag atgctacggc cagcaggatg
     4321 ggattgccca aagaaaaagt aatgatcaat attcagaaat acgggaatac tactgcagcc
     4381 actatcccac tctgtctttg ggaatggagg caccagctaa ataaaggtga taatattgtt
     4441 cttgctgcat ttggaggtgg atttacctgg ggagccacac tggtgaagtg ggcttactga
     4501 tttatcttaa tattttacct gcttatcaat gtccaggtca atatgacaac tgttgcagtt
     4561 cttaaccagc aggcgatatc ctttaatcgc tatcaccgta tcatttttcc ttataccgga
     4621 acgtatcagc ttaacaggtt ctttcatcct gtctttgtaa tannnnnnnn nnnnnnnnng
     4681 aaaaggggtc tttcagttta cggtgttcat caaattttcg gctcacctcc agcatgatgc
     4741 tatccatacc ttccagtaac caaaccgcat cctgcaaacg gcccgccttt aactcatctc
     4801 ccatattttc ctggtagatt ttcagatcaa gcatgttttc atcaaggtcc ttgatagtat
     4861 gcggacgatt atcccggcaa ctgctgcaag caacgaatat cgtcgcagat aataacaatg
     4921 atcttacaga tgaaagcatt caccaaatta aaacaacccg gttacttttc cattattatc
     4981 gatatcaata ttctccgccg caggaatagc cggcaagccc ggcatccgca tcatttcacc
     5041 taaaatcgga atgacaaacc ctgcgccgga tgcaaattca aattcccgaa cagttaccac
     5101 aaaattttcc ggcctgcccc gctttgtttc atcatcagat aatgatttgg gtgtttttgc
     5161 catacaaaca ggaatcttat caaatcccaa ttcctttatg tgcttcagat gtgttttggc
     5221 ttttacagaa aactccactc cccttgctcc gtaaatttcg gttgcaattt tgtgcatttt
     5281 ttcttcgata gtcatgtcag cctcgtaaag cggtttaaaa tcactttcac cattctctat
     5341 tatccgtacc acttctgctg ctaactctgt actgcctttt ccaccttcag caaaacattt
     5401 acaaacaatg gcttttactc catacttttt acaggcctgt tgcaccagtt ctatttcttc
     5461 ctggctatca ttgggaaaat aattgatcgc cacaactggc ttcattccga acttcatcag
     5521 gttctcaata tgtttttcca tattcggaat tcctttcagc agcttgtcaa tatttgccgt
     5581 ttgcaactca tcttttttag cgccaccatg atgacgtaat gcttttatcg taacaaccaa
     5641 tactgcagcc tccggcctta aaccggcgcc ggcacatttg atatcaaaga atttttcagc
     5701 acccaggtcg gcaccaaagc cggcttctgt aaccacataa tctgccaggg ataaacccat
     5761 tttggtagca ttcacagtat tcgttccctg tgcaatgctg gcgaaaggtc ctccgtgcat
     5821 aatgacaggg ttattctcca gtgtctgaac caggttgggt ttcatagcat ctttcagtaa
     5881 tactgccatt gccccgacag cattcaggtc tcttgcaaaa acaggctcac ccttatcatt
     5941 cataccgatg taaatattac ctaattttct tttcaggtca tcaatatctt ctgacaaaca
     6001 aagaatggcc atgatctccg aagcaggtgt gatgttgaat ccatcttctc tcggaatacc
     6061 ctggttgatt cctcctaatc caattaaaat atgacgcagt gcccggtcat tcatatccat
     6121 cacccgcttc cacacaattg ttctcggatc aatgtgcagg ttccttgttt tattctgcag
     6181 gttattatct ataagggctg ataaaagatt gtttgctttt tctacggcag aaaaatcgcc
     6241 ggtaaaatgc aggttgatgt cttccatggg cactacctgg gcataccctc cgcctgctgc
     6301 accgcctttt ataccgaata caggacccaa agatggttct cgtaatacag ccatggcttt
     6361 cttgccgatt acattcaacc catcagttag cccaatggaa acagtcgttt taccctcacc
     6421 atacggagtg ggtgtaatag cagttaccag caccaaatgg tgttgtgcaa tcttttcttt
     6481 atctattagc tttaatggaa gcttcgcttt gtattttcca tagtactcaa tatcatctgc
     6541 attaacacct atggaagcgg ccatttcttt tatatgcttc atttttgccg attgggctat
     6601 ctccaggtca gaagggaata ctgcaggcat aatcgttagt ttttaacact gcaacttagt
     6661 tgatagtact tttttggagc ttgataaata tcagaactgg atatgattta cttctgctgc
     6721 cgtacccaga taataaaatc cagtggaggt aacttatcaa ttcccagttg ccgaagtatg
     6781 gttaccaact ggcccctgtg gtaagtggaa tgattaaaaa catgcagcaa tacctggtat
     6841 acaggctgct taaacaactc ttttttggag ttatgaaaat gaaatacatg ccttagtgaa
     6901 agttctgaag cattctgtac ccagtcttcc cattgttttg actggttgat aagccctgta
     6961 aaaaggtcct gcatggagcc ggaaaagcta tctcttggaa aaataaccct ttcctgcatt
     7021 tttatacgct gccaccaacc act
//
LOCUS       DAORLS010000156         6884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5934_length_6884_cov_2.183775, whole genome shotgun sequence.
ACCESSION   DAORLS010000156 DAORLS010000000
VERSION     DAORLS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_6884_cov_2.183775"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PK977_11955"
     CDS             complement(<1..728)
                     /locus_tag="PK977_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRF18878.1"
                     /translation="MKETVSSFMAKATIKAADKEHRRKINFNIGKYNAVVPQGKQQFL
                     DVHLAREKAKNIKWKAIETLDKQLETFEAIITKRGAKVIWAEDAQQALEAIGKICEEK
                     NCKTLVKSKSMVTEEIHLNDYLEKQGIESVETDLGEYIQQLDGEPPYHIVTPAMHKSK
                     EDVARLFADKLGTDPKSTPEQLTLTARKVLREKYVQAEVGVTGANFIIADIGGIAVTE
                     NEGNARLSCSMPKTHIVIAGIEKV"
     gene            complement(736..1341)
                     /locus_tag="PK977_11960"
     CDS             complement(736..1341)
                     /locus_tag="PK977_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRF18879.1"
                     /translation="MIVIDNTLISDDVVEKQFVCDLTKCKGGCCEEGDAGAPLDEEEL
                     DIILAHYEKVKPYLTEASVKEIERKGKYVYHKEFGWVTPTLGNDKEICVYGRRDEKGV
                     IMCAFEQAYYDGVIPWKKPISCHLFPIIITRGKRGDYDKVNYEPREKLCNPACVPGKK
                     LQVPTYEFLKEPLVRKYGEEFYYAIDKVAKMKAAKKEKAVK"
     gene            complement(1373..>2046)
                     /locus_tag="PK977_11965"
     CDS             complement(1373..>2046)
                     /locus_tag="PK977_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18880.1"
                     /translation="GQLASCYNKSGDKKKAIEYFLKVKEISERNGMLENIKRSAKYLD
                     TLYKETGNLALSGTYNALYYQYKDSIETLKREKELTQVEAADEQQRQLRAEKEKEEAK
                     KRRNNIQYMAITIGIVGLFIALVVLGMFKVSRGLIKAIGFFVFLLFFEFIFLVFKKNI
                     YSITNGEPWKDLAFMIGLAAILVPLHHWLEHEVLKYLTSHNRLTSAGSHLKNKILSRI
                     KKEGK"
     assembly_gap    2047..2068
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2069..3194)
                     /locus_tag="PK977_11970"
     CDS             complement(<2069..3194)
                     /locus_tag="PK977_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF18881.1"
                     /translation="MRILSPFIILLLFAFSFSSLYGQEAAAVDSLKQALKEAKTVDEK
                     VTWLDELSRTMMNVNPKEADEYGRQLITVAEESRNRKLMFKAYLSNGLRCSYFATRKE
                     YSDKSIDFYNKAYDLARQNRMEKEAGIAQMHLAGIYLVIPDKEKALSYITQAFSLITT
                     LGDDSLKAEAHNMYGQVYMGKNENILALRNFLSALRIAEEMKDPGLMQSCYSKLSGFY
                     SSIEDYDKAIDYMMKSYEQFDGIKLKNTPYNRVIYINAIGNLYAYKKNYDIAISYFER
                     SIRMADSLKFSTLKVPGYVSLLNQYLRIDEPKKALDYLNSPAGGNLKQYLINFGMASI
                     IDQAYGVVYSEMGQYDSARIYLERAKPMFENSTNELSKVSF"
     assembly_gap    3408..3454
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3455..>3850
                     /locus_tag="PK977_11975"
     CDS             <3455..>3850
                     /locus_tag="PK977_11975"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRF18882.1"
                     /translation="SKNCDFIEELSFLFQSNLYNASFYIHIAAGALCIGTALIQFSRY
                     ILKKSKAIHRISGKIYVFVVLFLGAPTGLYMSFFAKGSFWERALFMFMAGWWFITTLY
                     GLSTIHKRNVVAHKIWMIRSYAMAMTAVTF"
     assembly_gap    3851..3875
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4048..>4234
                     /locus_tag="PK977_11980"
     CDS             4048..>4234
                     /locus_tag="PK977_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18883.1"
                     /translation="MKSFLYAGAALMIGASICGFVDYKNPSHKKEFNDMYAEKKAAKS
                     VVVTEEKVTTPVVEEKTE"
     assembly_gap    4235..4284
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4285..4490
                     /locus_tag="PK977_11985"
     CDS             <4285..4490
                     /locus_tag="PK977_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18884.1"
                     /translation="ARKEVITDEKKNPEEVTVAAVTEKKKEVKKKKRKISTEIFSRAP
                     LREEEEEILHPAKTDKKTVNKEL"
     gene            complement(4570..5193)
                     /locus_tag="PK977_11990"
     CDS             complement(4570..5193)
                     /locus_tag="PK977_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18885.1"
                     /translation="MFKKINSNRLAVIILILMSSCQLMVSCNSPYSAGKKKGYFHIDF
                     PEKKYKLFDQADYPYTFEYPVYANVIKDTSFFEDKPENPWWINIDVPQFAGRIHISYK
                     QIGGRNNFDSLVRDGFKMAYKQHVDVSTGINDSLMQTPNGIEGIYFSLGGNTATANQF
                     FLTDSTKHFLRGALYFDAAPNADSLGIVNDFLKQDLKHLINTLQWRK"
     gene            complement(5195..6541)
                     /gene="gldE"
                     /locus_tag="PK977_11995"
     CDS             complement(5195..6541)
                     /gene="gldE"
                     /locus_tag="PK977_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HRF18886.1"
                     /translation="METHPALYFLNDLFTLHFLAANPQGTTVLIVSLLLLLTLTFIIS
                     GAEVALFSLGTKDINVLKTKQHSAAKRIVNLLEEPKEVYASLLIAGTFVNISIIVLSN
                     FLIDQFISFKGMSTFIEIILKVLVIAFVLVFIGRMLPKVWATQKNFQFAYETSFVVEA
                     LHLLLRRISRWVVSLAERIGKKAGADKSAALSMQELDEAIDIKTDEEASQEEKNIMKG
                     IVKFGNITVKQIMRSRLEVSGVDHKLSFGELVKRVEELHYSRLPVYKGSLDEVVGMIN
                     TKDIIPFLGEPDNFDWHKLMRQPYFVPEPKLIEDLLKEFQQKRIHFAVVVDEFGGTSG
                     IVTMEDILEEVIGDIRDEFDDEESGNRKLDDNNYLFEGRTMIHDMCKAMKLPMDTFDD
                     VREESESLAGLVLELAGEIPAVNTVISAGDFEFTVIQADNNRVQQVKVTIKPRTKE"
     gene            complement(6653..>6884)
                     /locus_tag="PK977_12000"
     CDS             complement(6653..>6884)
                     /locus_tag="PK977_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRF18887.1"
                     /translation="AQKYLHKSSLVYIEGRLRTRSWDDKDGNRKFATEVVGDNLIMLD
                     KRMDGGGSTDGGMDNIGSPDMPMGDPSENLPF"
BASE COUNT         1805 a         1559 c         1344 g         2032 t
ORIGIN      
        1 attactttct caataccagc aatcacaata tgggtttttg gcatcgagca actcagtctt
       61 gcattgcctt cattctctgt caccgcaata ccgccaatat ctgcgatgat gaaattggca
      121 ccggtgacac ccacttccgc ctgtacatat ttttctctta gtacttttct tgctgtcagt
      181 gtcagttgtt ctggtgtaga cttcggatca gttcctaatt tatcagcaaa taacctggct
      241 acatcttcct tgctcttatg catggcagga gtaacgatat ggtaaggagg ctcgccatct
      301 agttgctgaa tatattcccc caggtcagtc tccacacttt ctattccttg cttctccaag
      361 taatcattca ggtgaatttc ttccgtcacc atgctcttgc tcttcaccag tgttttgcag
      421 ttcttttcct cacaaatttt tccaatagct tccaatgctt gttgtgcatc ttctgcccag
      481 atgactttgg cgccacgttt tgtaatgatg gcttcgaatg tttccagttg tttatccaat
      541 gtttcaattg ccttccactt gatattcttg gctttctccc ttgccaggtg aacatccaga
      601 aactgctgtt taccctgagg cactaccgca ttgtatttac caatattgaa attgatcttg
      661 cggcgatgtt ctttgtccgc tgccttgatg gtggctttcg ccatgaaact ggaaactgtc
      721 tctttcactg gtttactatt taaccgcttt ttctttttta gccgccttca tctttgccac
      781 tttgtcgatg gcatagtaaa attcttcgcc atatttacgg accaggggct cttttaaaaa
      841 ttcatatgtt ggtacctgca atttcttgcc gggtacacag gctggattgc aaagcttttc
      901 ccttggttca taattcactt tatcataatc acctcgttta cccctggtga taatgatggg
      961 aaagagatgg cagctgatgg gctttttcca cggaataaca ccgtcataat atgcctgctc
     1021 aaaagcacac atgataacac ccttttcatc ccgacggcca tacacacaaa tttccttatc
     1081 attacccagg gtgggtgtta cccagccaaa ttccttgtga taaacatatt tgccctttcg
     1141 ttcaatttct ttgacagatg cttccgtcag gtagggcttt accttttcgt aatgcgccag
     1201 aatgatatcc agttcttctt catcgagcgg ggcaccggca tcaccttctt cgcagcagcc
     1261 gcctttacat ttggtaaggt cgcaaacaaa ctgcttctcc accacatcat cactaatcag
     1321 ggtattatca ataacaatca aggggtaaaa ttttagcaaa ggtaagcgaa ggttatttcc
     1381 cttccttttt aatacgactg agtattttgt ttttcaggtg agaacctgct gaagtaagcc
     1441 tgttatgcga tgtgaggtac ttcagcactt catgctccag ccagtgatgc agtggtacca
     1501 atatagccgc caggccgatc ataaaagcaa ggtctttcca cggttcacca ttggtaatag
     1561 aataaatatt tttcttgaat acaaggaaga tgaattcaaa gaaaagcagg aatacaaaga
     1621 acccgatagc tttaatgagt cccctcgaca ctttgaacat acccagcacc accagggcga
     1681 taaacaaacc aacaattcct atcgtgatag ccatgtactg gatattattg cggcgttttt
     1741 tggcttcttc tttttctttt tcagccctca gctgtctttg ttgctcatcg gccgcttcta
     1801 cctgtgtcag ttctttctct ctttttagcg tttcaatact gtctttatac tggtagtaca
     1861 gggcattata agtacctgac aaggccaggt tgcccgtttc tttatataac gtatcaagat
     1921 atttggcaga ccgctttatg ttttccagca taccgtttct ttcactgatc tcctttactt
     1981 tcaggaaata ttcaatggct ttctttttat cgcccgattt attgtaacat gatgccagct
     2041 ggccatnnnn nnnnnnnnnn nnnnnnnnag aaactcactt ttgacaattc gttggtgcta
     2101 ttttcaaaca tgggcttagc cctttccagg tatatacggg ccgaatcata ttgccccatc
     2161 tctgaataga ccacgccgta ggcctgatct ataatcgaag ccataccaaa attgatcagg
     2221 tattgtttaa gattacctcc tgcaggcgaa ttaagataat ccaatgcctt tttgggctca
     2281 tctatccgca ggtactggtt cagcaggctt acatatccag gcactttaag ggtagaaaac
     2341 tttagcgaat cagccatccg gatagaccgt tcaaaatagc tgatggcaat atcatagttc
     2401 ttcttgtagg catacaggtt gccaatagca ttgatataaa tgaccctgtt atagggtgtg
     2461 ttttttaatt taataccgtc aaactgctca taggacttca tcatataatc aatggcctta
     2521 tcataatcct cgatcgaact gtaaaagcca gaaagtttgg aatagcaact ctgcatcaaa
     2581 cccgggtctt tcatttcctc tgcaatacga agtgcactta gaaaattacg cagggcaagg
     2641 atattctcat ttttgcccat gtaaacctgt ccatacatgt tgtgggcctc ggctttaagg
     2701 ctgtcgtcac ccagggtagt aataagcgaa aaagcctgcg tgatataaga gagggctttt
     2761 tctttatctg gtatcaccag gtaaatgccg gccaggtgca tctgggcgat gccggcttct
     2821 ttttccatcc ggttttgcct ggccaggtcg taagctttgt tataaaaatc aattgactta
     2881 tcggaatatt cttttcgggt ggcaaaataa ctgcagcgaa gaccgttgga aagataggcc
     2941 ttgaacatca gcttacggtt acgggattct tcggctactg tgatcagttg ccggccatat
     3001 tcatccgcct ctttgggatt cacattcatc atcgtccggc tgagctcatc cagccaggta
     3061 accttttcat ccacagtttt tgcttctttc aaagcctgtt tcagggaatc aactgcagca
     3121 gcttcctgtc catacaatga gctgaagctg aaagcaaaaa gtaaaagaat aatgaacggt
     3181 gaaagaatcc tcatggcgtt gatttattcc tcaatttagt gaatcggtga aagggagtaa
     3241 aattccttta acaaacatgt aacagtggtc gtttatggac agttttagtc accagcatct
     3301 ctcccctatc acaaagccgc tgtatgcaaa acacaagatt gctcacattt cagaaatcca
     3361 tccgggtttt ctcacaaatg ctcttctggg ttcctgttgc cgcatttnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcaag aattgtgatt tcattgaaga
     3481 actcagcttt ctttttcaaa gtaaccttta taatgcaagt ttctatatac atatcgctgc
     3541 cggtgccttg tgtattggaa cggcactgat acaattctcc cgttatatat tgaagaagtc
     3601 taaagccatt caccgtattt caggaaagat atatgttttc gttgtgttgt tcctgggggc
     3661 gcctactggt ctgtacatgt cgttttttgc caagggtagt ttttgggaaa gggctttatt
     3721 catgttcatg gcaggctggt ggttcattac tacactgtac ggattaagta ctattcataa
     3781 aagaaacgta gtggcacata aaatatggat gatacgcagc tatgccatgg caatgactgc
     3841 cgttacattc nnnnnnnnnn nnnnnnnnnn nnnnnacctg ctgggatggg gccacctgga
     3901 aaattatgaa gtatcactct ggatcagtgt aattggcaat atgctttttg cagaatgggt
     3961 gatctggcgg caaagcaaac aataccttaa gtcatttgcg acataaacta agcaagtaat
     4021 caagttaaac attaaaacaa acaaccaatg aaatcattct tgtacgccgg cgcagcgtta
     4081 atgatcggcg ccagtatctg cgggtttgta gattacaaaa atcccagcca caaaaaagag
     4141 ttcaacgaca tgtatgcaga gaagaaagcc gcaaaatctg ttgtggtgac agaagaaaaa
     4201 gtaaccaccc ccgttgtgga agaaaagacg gaacnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnttgccc ggaaagaggt aataaccgat gaaaagaaaa
     4321 atcctgaaga agtaactgtt gctgctgtaa ctgagaaaaa gaaagaggta aagaagaaaa
     4381 agagaaagat cagtaccgag attttcagcc gggccccatt gagagaagag gaggaagaaa
     4441 tccttcatcc tgctaaaact gataagaaga cggtgaacaa agaattataa tcagctcaca
     4501 ggatcacaca gcattaatcc accctcagca tacggtgatt tgacccccgg ttagcgatag
     4561 cagccggggt tattttctcc actgcaacgt gttgatgaga tgttttagat cctgtttcaa
     4621 aaaatcattg acaattccga gtgagtctgc gtttggtgcc gcatcaaagt ataaagcccc
     4681 tcttaaaaaa tgcttagtag agtcagtcag gaagaactgg ttggctgttg ctgtattccc
     4741 gcctagcgag aaataaatac cttctattcc attgggtgtc tgcatcaggc tgtcattgat
     4801 accggtggaa acatccacat gttgtttata agccatcttg aaaccatctc ttaccagtga
     4861 atcaaaatta ttcctgccac caatttgttt atagctaata tgaattcttc cggcaaactg
     4921 cggtacatca atattgatcc accaggggtt ttctggcttg tcttcaaaaa aagaagtgtc
     4981 tttgatcaca ttagcataaa cagggtattc aaatgtataa ggataatccg cctgatcgaa
     5041 taacttgtat tttttttcag ggaagtcaat atgaaagtat cccttctttt tacctgccga
     5101 ataggggcta ttgcaactta ccattaactg gcaggatgac atcagtatta aaattattac
     5161 tgccagccgg ttgctattta tctttttaaa catgctattc tttcgttctg ggtttgatag
     5221 ttactttaac ctgctgcacc cggttattat ctgcctgtat tacggtgaac tcaaaatcac
     5281 ctgctgaaat cacagtattc acggccggta tttcacctgc cagttccagt accaggcccg
     5341 ccagtgattc actttcttcc cgcacatcat caaacgtatc catcggaagt ttcattgctt
     5401 tgcacatatc atgtatcata gtgcggcctt caaataaata attgttgtca tccagtttgc
     5461 ggtttccgct ttcctcgtca tcaaattcat cccgaatatc accaatcacc tcttccagta
     5521 tatcttccat cgtaacaata ccgctggtgc cgccaaactc atccaccacc acagcaaaat
     5581 gtatccgctt ttgctgaaac tctttcagca aatcttcgat cagctttggt tcgggtacaa
     5641 aataaggttg ccgcatcagc ttatgccagt caaaattatc cggctcacca aggaagggaa
     5701 taatgtcctt ggtattgatc atgccgacta cttcatccag gcttccttta tacaccggca
     5761 gccgggagta atgcaattcc tccacccttt tcaccagttc gccgaaagag agcttgtgat
     5821 ccacaccact gacttcgaga cggctccgca tgatctgttt caccgtgata ttgccaaatt
     5881 ttacaattcc tttcatgatg ttcttctctt cctgggatgc ttcttcatct gttttgatat
     5941 caatcgcttc atccagctcc tgcatgctaa gggccgcaga tttatcagcc cctgcctttt
     6001 tacctatccg ctctgccaat gacacgaccc atcggctgat gcgacggagt aacaagtgca
     6061 atgcctctac tacaaaagaa gtttcataag caaactgaaa gttcttttgg gtggcccaca
     6121 cttttggcag cattctgccg ataaatacca atacaaaagc aatcaccagc actttcagga
     6181 tgatctctat aaaagtgctc atgcctttaa aagatataaa ctggtctatc aggaaattgg
     6241 acagcactat gatactgata ttgacaaaag ttccggcaat aagcagtgag gcatatactt
     6301 cttttggctc ttccagcagg tttactatgc gtttggcagc gctgtgctgt tttgtcttta
     6361 gtacattgat atccttggta ccgagagaga acagagccac ttcagcgccg gaaatgataa
     6421 aagtaagtgt cagcagcagt agtaatgata cgatcaatac tgtggttccc tgcgggttgg
     6481 ccgccaggaa atgaagcgta aacagatcat tgagaaaata cagtgccggg tgagtttcca
     6541 aaactggttt tttggttcaa taaaaaggca ggtcgtatgg cccgccagca aagcgacaaa
     6601 gttattgtat ttggaattgt tacccgcaat gggatccttt cttccacaaa ctctaaaacg
     6661 gcaaattctc tgaagggtcc cccatgggca tatcaggact accaatgtta tccattcccc
     6721 catcggtgct gccaccacca tccatccgtt tgtcgagcat aatgaggtta tcacccacca
     6781 cttcggtggc aaacttgcga ttgccgtcct tatcgtccca gctgcgggta cgcaggcggc
     6841 cctcgatata caccaggctg cttttgtgca ggtatttttg tgcc
//
LOCUS       DAORLS010000157         6884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5935_length_6884_cov_1.915215, whole genome shotgun sequence.
ACCESSION   DAORLS010000157 DAORLS010000000
VERSION     DAORLS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5935_length_6884_cov_1.915215"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1204)
                     /locus_tag="PK977_12005"
     CDS             complement(<1..>1204)
                     /locus_tag="PK977_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HRF18888.1"
                     /translation="FKFNFGAPSCVPATSFETAGAVLDSTDVEHLLQREEIKYLSEMM
                     NFPGVLFKDEEVMRKIAAAHRLGKPVDGHAPGLRGKQAKKYIDAGIYTDHECFTSEEA
                     LDKLRYGMKIIIREGSAAKNFEALIGLLNDWPDKMMFCSDDKHPDSLVLGHINQLCAR
                     AVAKGINIFKILKAACVNPVEHYKLDVGLLREGYPADFIIVKDLVKFEVVKTYINGEL
                     VAENGKSLIRTQESGVINNFTCSKRNVEDFAVVWNGEKEIPAIEALDGQLITNKLFFE
                     PKVVDGKIVSDTDRDILKIVVVNRYSDAPVAKAFIKNFGLKQGALASSVAHDSHNIVA
                     VGVDDDSICRAVNKVIEKKGGVSVATNYQSPDGKLSMQAESVVALAVAGLMSNEDGYI
                     VAEAYTEID"
     assembly_gap    1205..1275
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1276..1522)
                     /locus_tag="PK977_12010"
     CDS             complement(<1276..1522)
                     /locus_tag="PK977_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF18889.1"
                     /translation="MSSFSVSGNFVDILHEKIYPATIKVENGKIISIQPNNQLTNKPL
                     NYILPGFIDSHVHIESSMLVPSQFARLAVVHGTVATVS"
     gene            1595..>3721
                     /locus_tag="PK977_12015"
     CDS             1595..>3721
                     /locus_tag="PK977_12015"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18890.1"
                     /translation="MRKIILLAFAITPFFDSVAQKTKTSPPINTDSKDDVLLKNVKYR
                     LIGPFRGGRSAAVAGSYSNKTTFYFGGTGGGVWKTVDGGSNWINISDKYFGGSIGAVA
                     VAPSDETILYAGEGENTMRGNVSEGLGGMWRSDDGGRSWKNIGLKDSRHILRIIIHPK
                     NPDIVWVAAMGHLFGPNEERGIFKTADGGKTWKKTLFVNNQTGCSDLVMEPGNPSVFY
                     AGTWRLIRTPYSLESGGEGSGLWKSTDGGETWTNISAKKGLPKNIWGIVGVAVAPSNT
                     DKVYAIIENKDGGLFMSADGGETWSLTSNDNNIRQRAWYYTKVFVDPKNENKVYCPNV
                     DFMVSTDGGKTFKGISTPHGDHHDLWIDPQDGNRMVVADDGGAQVSFEGGANWSTYLN
                     QPTAQVYRVSTDNAFPYRIMGGQQDNSAFRIRSRTYGSAITAADMETAAGSESGYVVA
                     DPLNPDITYGGNYMGMLQRLDHRTGESRLINVWPVDNMGAGAEAAKYRFQWNYPIFFS
                     PHNPKRLYAAGNHLFVTENEGKSWEQISPDLTTNDKSKQASSGGPVTQDNTSVEYYCT
                     IFTAAESPYEKDLLWTGSDDGLIHVSRDGGKNWENVTPKDCPQWMMWNCIEADPFKKG
                     AAYFVGTRYKADDFRPYIYKTEDYGKTWKLITTGIPSLHFTRAMRADRVKQGLLYAGT
                     EYGMYISYDGGNNWKPFQLNLPVTPIT"
     assembly_gap    3722..3753
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3754..4750
                     /locus_tag="PK977_12020"
     CDS             <3754..4750
                     /locus_tag="PK977_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18891.1"
                     /translation="IVATQGRSLYILDDLSVIQQKSESVLSKNLHVFEVNPAYRMPGG
                     GRRGGGAMASSSVRNAGINPSNGVVINYYLKNATDSTKLSIEILDKKKQKIKTFSTSS
                     KEDKIAVVNGMNQFEWDMNYPVAERMDGLVLWNGFVRGPKAAPGEYLAKFKSGSDSVE
                     VKFTILADPNYKTTQTEYEEQVNFLLTVRDKFSDVIKALKNIKELKQQMTDLTARAGK
                     GMPKEIKQKIDTITKQMTAVEEALHQTKAKSSQDVLNYPIRLDDKLASTYNAAAAGNT
                     APSKQVKQAYAELEMLINAELAKLKRVMDEDVIQLNKMIYEKEVPVIGIKKEEKE"
     gene            complement(4787..>5671)
                     /locus_tag="PK977_12025"
     CDS             complement(4787..>5671)
                     /locus_tag="PK977_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF18892.1"
                     /translation="VLLYTPVLQGWYWWLVYVPYFYISQMYFKGRFGLMLHCICHRKM
                     FKKAYTWMFHYIIWIVCPFFGHTPETYFAHHMGMHHVENNMLDDASSTLPYQRDSFRG
                     FLSYVGRFLILGFRDTFMYFFSRKRKKFYMRLTIGEMAFYLFCIGMSFVNFHATLFIF
                     IIPFVFARIVMMLGNWTQHAFVDLENLEDNTINCINTKYNKACWNDGYHAVHHVRPAL
                     HYTEIPGEFLKNKEFFVKQKTLTFDGIHYLHIFFWLMTKRYDKLAKHVVNVDNMFSSE
                     EEVIALMKERTRKYIPSK"
     assembly_gap    5672..5698
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5988..>6884)
                     /locus_tag="PK977_12030"
     CDS             complement(5988..>6884)
                     /locus_tag="PK977_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylgalactosaminidase"
                     /protein_id="HRF18893.1"
                     /translation="HCMMLENCCYDFFELLTLNMTRQGMFGEIVHAEGAYIHDLSKDW
                     LFNKKAYADMWRLKENIGHNGNLYPTHGLGPIAQCMDINRGDKFDHLVSMSTNDFTLN
                     NMAKEMAAKDEFFKPYVDQPYRGNMNTTLIRTHKGKTVMVQHDVSTIRPYSRIHLVSG
                     TKGAAQKWPGPQRIAFGHSWIKTEELKQLEEKYAPPIVKHIGNIAKEVGGHGGMDFIM
                     DWRLIDCLRNGLPLDQDVYDAAAWSCLMPLSERSVAKKSRTIDIPDFTRGAWQSNRPV
                     DITLNGGATTGVRNIKPELKMK"
BASE COUNT         1987 a         1373 c         1497 g         1897 t
ORIGIN      
        1 gtcgatctcc gtataagctt ctgctacaat atagccatct tcgttactca tcagcccggc
       61 tactgccagt gctactactg attcagcttg catacttagt tttccatccg gcgactgata
      121 attggtggct acacttacac cgcctttttt ttcaatcacc ttgtttacag ccctgcaaat
      181 actgtcatca tccactccaa cagctacaat attgtgactg tcatgtgcca cagaagaagc
      241 taatgcaccc tgcttcaatc cgaagttttt aataaaagct ttggctaccg gggcatctga
      301 ataccggttc accacaacga tctttaatat atctctgtca gtgtcgctca ctatctttcc
      361 gtccacaact tttggttcaa agaataattt attagtaatg agctgaccat ctaatgcttc
      421 aatagccggt atctcttttt caccattcca tacaacggca aagtcctcca cattcctttt
      481 actgcaggtg aagttgttta ttactcccga ctcctgagtc ctgattaagg actttccatt
      541 ctcagctacc agttcaccat tgatataggt ttttactact tcaaatttta ccaggtcttt
      601 tactataata aaatctgcag gatatccttc tcttaacaag cccacatcca atttataatg
      661 ttcaaccggg ttcacacagg cagctttcag tattttaaaa atattaatgc ccttggctac
      721 tgctcttgca caaagctggt tgatatggcc gagtaccagg ctgtcggggt gtttatcatc
      781 gctgcagaac atcattttat ccggccaatc attcagcagt ccaatcaatg cctcaaaatt
      841 tttggcggcg ctgccctccc ggatgatgat cttcataccg tatcggagtt tatccaatgc
      901 ttcttcggaa gtaaaacact catgatcagt atagatacct gcgtctatat atttttttgc
      961 ctgttttcct cttaagcctg gtgcatgacc gtctactggt tttcctaacc ggtgagcagc
     1021 ggctattttt ctcatcacct cttcatcttt aaatagcact ccggggaaat tcatcatctc
     1081 actcaggtac tttatctctt ctctttgcag cagatgttct acatcggttg aatcaagtac
     1141 tgccccggcc gtttcaaaag atgtggcagg cacgcaactg ggtgctccga aattgaattt
     1201 gaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnncacta acagtggcta ctgtgccgtg tacaaccgcc agccttgcaa
     1321 attgggaagg aaccagcatt gagctttcga tgtggacatg gctgtcgata aaaccgggga
     1381 gaatatagtt gagaggttta ttggttaatt gattattggg ctgaatggaa atgattttcc
     1441 cattctcaac ttttattgtt gccgggtaaa tcttttcatg tagtatatct acaaaatttc
     1501 cactgacaga aaacgatgac ataaggataa tttggttaaa gttaaatcag taacttcaaa
     1561 cggaatttaa attggttaag actaactaag ttatatgaga aaaataatcc tgctcgcctt
     1621 tgcgattact cccttctttg attctgttgc acaaaaaaca aaaacgtctc ctccgattaa
     1681 tactgacagc aaggatgatg ttctgttgaa gaatgtaaag taccgtctga tcggtccatt
     1741 ccgtggtggc agaagtgcag cggttgccgg cagttattcc aacaaaacaa ctttttattt
     1801 tggcggcact ggtggcggtg tatggaaaac agttgacgga ggcagcaact ggataaatat
     1861 ttcggataag tattttggcg gatctatcgg ggctgtggca gtagcaccct cagatgaaac
     1921 cattttgtat gcaggtgaag gtgagaatac catgcgggga aatgttagcg aaggattggg
     1981 cggtatgtgg cgaagtgatg atggtggccg tagctggaaa aatattggcc tgaaagacag
     2041 ccgtcatatt cttagaatca ttatccatcc caaaaacccg gacattgttt gggtagcagc
     2101 gatgggccac ctgttcggac ctaatgaaga aagaggaata tttaaaacag ctgatggcgg
     2161 taaaacatgg aagaaaacgc tttttgtaaa taaccaaacc ggctgcagtg acctggtgat
     2221 ggagccgggc aacccttctg ttttttatgc tggcacctgg cggcttatcc gaacacctta
     2281 ttcattggag agcggaggcg aaggaagcgg attatggaaa agtaccgatg gtggtgaaac
     2341 ctggacaaat atttctgcaa agaaaggatt gcccaaaaat atctggggta ttgtaggggt
     2401 agctgttgcg ccttccaata cggataaagt gtatgccata atagaaaata aagatggtgg
     2461 attgttcatg agtgctgatg gtggcgaaac ctggtcactt accagcaatg ataataatat
     2521 ccgccaacgg gcatggtatt acactaaagt ctttgtagat ccaaagaatg agaataaagt
     2581 gtattgtcct aacgtggatt ttatggtaag tacagacgga ggcaagacgt ttaagggaat
     2641 atctactccg catggtgacc accatgatct ctggatagat ccgcaggatg gaaaccggat
     2701 ggttgtggcg gatgacgggg gagcacaagt gtcattcgag ggtggtgcca actggagtac
     2761 ttaccttaat cagcctactg cacaagtgta cagggtaagc acagataatg catttccata
     2821 ccgcattatg ggcggccagc aggataatag tgcctttcgc atccggagca gaacttacgg
     2881 atcagctatt acagcagctg atatggaaac agctgcagga agcgaaagtg gttatgtggt
     2941 agccgatcct ttaaatcctg atattaccta tggaggtaat tatatgggta tgttgcagcg
     3001 gctcgatcac cggaccgggg aaagcaggct gatcaatgtg tggccggtag ataatatggg
     3061 cgccggtgca gaagcagcga aatatcgctt tcaatggaac tatccgattt tcttttctcc
     3121 gcataatcct aaacgtcttt atgcagcggg taaccattta tttgtaactg aaaatgaagg
     3181 aaagagctgg gagcagattt cacctgatct taccacgaat gataaaagca agcaagcatc
     3241 cagcggcggc ccggtaacgc aggataacac atcggtagaa tattactgta ccatttttac
     3301 tgctgccgag tctccctatg agaaggatct cttgtggacc ggaagtgatg acggtttgat
     3361 acatgtaagc cgggatggag gaaagaactg ggagaatgtt actcccaaag attgccctca
     3421 atggatgatg tggaattgca tagaagccga tccttttaaa aagggggctg cttattttgt
     3481 aggtacccgt tacaaagcag atgattttcg tccctatatc tacaaaacgg aagactatgg
     3541 taaaacatgg aaattaatca ctactggtat tccgtctttg cacttcaccc gggctatgcg
     3601 tgcagacagg gttaaacaag gtttattata tgccggtaca gagtatggta tgtatatttc
     3661 atatgatggc ggtaataact ggaaaccttt tcagttgaac ttgccggtaa caccaatcac
     3721 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatcgtt gctacgcagg gtcgtagcct
     3781 ttacatcctg gatgacctgt cggttataca acagaaaagt gagtctgtac tttcaaaaaa
     3841 cctgcatgtg tttgaagtaa acccggctta tcgtatgcct ggaggcggac gaagaggtgg
     3901 gggtgcaatg gcatcatcaa gtgttcgaaa tgcaggtata aacccttcca atggtgttgt
     3961 tattaattat tacctaaaga atgcaactga ttcaacaaag ctttctattg aaatccttga
     4021 taagaaaaag cagaagatca aaacattctc tacttcttca aaagaagata aaatagctgt
     4081 tgtaaatggt atgaaccagt ttgaatggga tatgaattac ccggtagcag aaagaatgga
     4141 tggccttgtt ttatggaacg gttttgtgag gggacccaag gcagcgccgg gtgagtattt
     4201 agcaaaattc aagtcaggat cagattctgt agaggtaaaa tttacgatcc tggccgatcc
     4261 gaattataaa acaacccaga cggaatatga agagcaggta aacttcctgc taaccgtgag
     4321 agataaattt tctgatgtaa tcaaagcatt aaagaacatc aaagaactaa aacagcagat
     4381 gaccgatctt actgcaaggg cggggaaggg aatgccaaag gaaatcaaac agaaaataga
     4441 tacaattacc aagcaaatga ctgcagtgga agaagccctt caccagacaa aagcaaagag
     4501 cagccaggat gtattgaact atcccatccg gttagatgat aagctggcca gtacttataa
     4561 tgcagcggca gcgggtaaca ctgctccttc caaacaagta aaacaggctt atgcagaact
     4621 ggaaatgcta atcaatgccg aattagcaaa gctaaaaagg gtgatggacg aagatgtgat
     4681 acagcttaat aaaatgattt atgaaaagga agtacctgta ataggcataa agaaagaaga
     4741 aaaagaataa aataaaaggg cttcatttga agccctttta ttttagttat ttagacggga
     4801 tatattttct tgttctttcc ttcatcagag cgatgacttc ttcttcgctg gaaaacatgt
     4861 tgtcaacgtt tactacatgt ttggctagtt tgtcataacg ctttgtcatc agccagaaga
     4921 aaatatggag gtagtgaatg ccgtcgaaag tcagtgtctt ttgttttaca aaaaattctt
     4981 tgttcttcag aaactcaccc ggtatctcgg tatagtgtaa tgccggacgg acgtgatgta
     5041 ctgcatgata gccgtcattc cagcaggcct tgttatactt ggtgttgatg cagttgatgg
     5101 tattgtcttc taaattctca aggtcaacaa aagcatgttg tgtccagttt cccagcatca
     5161 ttacaatcct ggcgaataca aacggaataa taaaaataaa caaggtagca tggaaattaa
     5221 caaagctcat cccgatgcag aacaggtaaa atgccatctc accgattgta aggcgcatgt
     5281 agaatttttt tctcttcctg ctgaagaaat acatgaaagt gtctcggaag ccaaggatga
     5341 gaaaccgccc gacataggac aaaaagccgc ggaagctgtc cctttgataa ggtaatgtgc
     5401 tgctggcatc atccagcata ttgttttcaa cgtggtgcat acccatgtga tgggcgaaat
     5461 aggtttcagg ggtatgacca aaaaaagggc aaacgatcca gatgatataa tggaacatcc
     5521 aggtgtaggc ctttttaaac atcttgcgat gacagataca atgcagcatt aatccaaaac
     5581 ggcccttaaa atacatttgc gaaatgtaga aataagggac gtaaaccaac caccaatacc
     5641 agccctgtaa aacgggtgta tataaaagaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     5701 tgtgaatagc ggtcagcaaa tgaataaaag gtagatccct tttatcaagc atgaatttga
     5761 gccaaaacct gtcataagca gagaattctt tcggttcagt gtagacggga tcggtaatta
     5821 ctgtcagtgc cttcatttat agctaattat aaaaaccaga tgatagtgct tataaaaatt
     5881 ctgccagggg gcaaatgaat taaaacgggc taaaattaag ctattatcga ttatctgccg
     5941 gaattgttta attaaaaaca aggatgattc tgatcagtaa aacagcctta tttcatcttt
     6001 aactccggct ttatgttcct tactccggta gttgcacctc catttagtgt tatgtctaca
     6061 ggcctattgg actgccatgc tcccctggta aaatcaggga tatcgatagt tcttgatttt
     6121 ttggctactg acctttcact cagtggcatc aggcaactcc aggcggccgc atcgtatacg
     6181 tcctggtcaa gaggaaggcc attgcgaagg caatcaataa gccgccagtc cataataaaa
     6241 tccataccgc catggccgcc tacttctttg gcgatattgc caatatgttt cacaataggc
     6301 ggggcatatt tttcttcgag ttgtttcaat tcttctgttt ttatccagct atgaccaaag
     6361 gcaatgcgct gtggaccagg ccatttttgt gctgcacctt ttgtgccgct caccaggtga
     6421 attcttgaat aaggccggat agttgaaaca tcatgctgta ccatcaccgt ttttccttta
     6481 tgggtacgga ttagagtggt gttcatattg cctctgtatg gttgatcaac atatggcttg
     6541 aagaattcat ctttcgccgc catttctttt gccatattgt tcagggtaaa atcattggta
     6601 ctcatactta ccaggtgatc aaatttatcc cccctgttaa tatccataca ttgggcaatg
     6661 ggtccgaggc catgcgttgg atagagattt ccattgtgcc cgatattttc tttcagccgc
     6721 cacatatcag cgtaggcttt tttattaaat agccaatcct tactcaggtc atgaatatag
     6781 gcgccttcgg catgaacaat ttctccaaac attccttgtc ttgtcatgtt cagtgtaagg
     6841 agttcaaaaa aatcatagca gcaattctca agcatcatac aatg
//
LOCUS       DAORLS010000158         6851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5972_length_6851_cov_2.210712, whole genome shotgun sequence.
ACCESSION   DAORLS010000158 DAORLS010000000
VERSION     DAORLS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5972_length_6851_cov_2.210712"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    208..243
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            492..573
                     /locus_tag="PK977_12035"
     tRNA            492..573
                     /locus_tag="PK977_12035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:526..528,aa:Leu,seq:tag)
     gene            655..>1256
                     /locus_tag="PK977_12040"
     CDS             655..>1256
                     /locus_tag="PK977_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HRF18894.1"
                     /translation="MATVTKENIGLLHEKLTVKLEKTDYLPSFEKALKEYSKKANIPG
                     FRKGMVPAGLIKKMYGPSLFTDEVLKSVDRELINYLQNDNLDIFAQPLPMESDFRQLD
                     VNNPVDYTFHFEIGMKPSFQLPDLGKAKITRHVVKVTEEMIDNEVTRLQNRYGNMKDE
                     DSINSDDNVLNIVLTETDAEGNETEGGIKKDNSVLLKYFA"
     assembly_gap    1257..1269
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1270..2044
                     /locus_tag="PK977_12045"
     CDS             <1270..2044
                     /locus_tag="PK977_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRF18895.1"
                     /translation="ENFRNKWMGKKVGDAEVAQLKSAFDDKEREWIISDLGLKDDISA
                     ADKYFKIEITKIGIQEKRELNEEFFNQLFPNQSVVTEGEFRSKVKDEIQAYWNGQAMN
                     QIHDQVFHELVDHTNIEFPEGFLKKWVKSQGETQKSDEEVEAEFPKFLSQLKWTLISD
                     KIVQDNGIQVNPDEIRNFAKQQLFSYMGGANLSDDQPWVNDYVEKMMKDRKYVEDSYN
                     RIQTQKIFEWAAGQVNPADKEISAEDFTKMVEEHQHHHH"
     gene            2304..2996
                     /locus_tag="PK977_12050"
     CDS             2304..2996
                     /locus_tag="PK977_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRF18896.1"
                     /translation="MSFNSEFEKYAVKHHGISSNKLNSYASHLVTAMTPNIIEERSLN
                     VAVMDVYSRLMMDRIIFLGYPINDEIANIVTAQLLFLDSTDRSRDINMYINSPGGSVY
                     SGLGVYDTMQYVSPDIATICIGVAASMACVLLGAGTKGKRAALKHSRIMMHQPSGAIG
                     GQASDIEITVNEIRKLKRELYEVVNIHTGKPIDQIEKDFDRDNWMTASEAKDYGLIDE
                     VLLTNPRKQKKD"
     gene            3024..3653
                     /locus_tag="PK977_12055"
     CDS             3024..3653
                     /locus_tag="PK977_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRF18897.1"
                     /translation="MNRKQFIYNDWKAEDEEEKEEESKSDIGMFGKKLEKLFFEKRAI
                     YLWGVVDDKSAKDVVSKLLLLDADKPGEEIKFFINSPGGVVTSGMVMYDTMQMIKSPV
                     STICMGLAASMGSILLSGGVKGKRYIYPHGEVMIHQPSLGGYIQGVSADLEIQARQTL
                     RVKEIGASILAKNCGKTVDQIMKDFARDYWMDAKQAIEYGIVDGIVESL"
     gene            3792..5030
                     /gene="clpX"
                     /locus_tag="PK977_12060"
     CDS             3792..5030
                     /gene="clpX"
                     /locus_tag="PK977_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRF18898.1"
                     /translation="MAKNPLHCSFCGRSRDEVKILIAGQEGHICENCVEHAREIIDQE
                     LAIKDDRSNSSFKLTVKKPVEIKKFLDEYAIGQDEAKKVLAVAVYNHYKRLQQKTADN
                     GNDIEIEKSNIIMVGETGTGKTLLAKSIARMLNVPFAIVDATVFTEAGYVGEDVESIL
                     TRLLQVCNYDVAAAERGIVYIDEIDKIARKGDNPSITRDVSGEGVQQGMLKLLEGTDV
                     LVPPQGGRKHPEQKLIKINTQNILFICGGAFDGIDKIIARRVQTNTIGFNVDKEMQET
                     MKKNLLRYVNATDLKAFGLIPELLGRLPVVTHLDPLDATTLRAILTEPKNALVKQYKK
                     LFELEGIQLTIEEDVLDFMVEKAMEYKLGARGLRSICEGILIDAMYELPSSKETQFTL
                     DLEYAKRKFDKSKLSLLKVA"
     assembly_gap    5223..5241
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5652..>6851)
                     /locus_tag="PK977_12065"
     CDS             complement(5652..>6851)
                     /locus_tag="PK977_12065"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRF18899.1"
                     /translation="AANDYVCGTMMLEGAPGLKEEHLPVFDCANKCGQKGQRYIHAHG
                     HIRMMGAVQPFISGAISKTINLPHEAEVGEIAEAYHLSWKLGLKANALYRDGSKLSQP
                     LSNKSDKKKKTGDEKSTVDSQQSAANEQLASANESNIVDLGKLTVEELLEEVQKRVQA
                     SPDTQLKRKLASIVERRTLPAKRRGFVQKAKINGQAVFLRTGEYSDGTLGEIFIDMAK
                     EGATMRSMMNCFAISISIGLQYGVPLEEFIDKFAFTRFEPSGFVEHPNIRTTTSIVDF
                     IFRVLGYEYLGRTDLVHVLDKPEVMNTGSDDWDEIPSNGLEYAKDPELSSVRITPSSG
                     TVAPQPVRNQRTAQTVKADAGTGAMNEALKGMQSDAPACNVCGNIMMRSGACYKCLNC
                     GNQGGCG"
BASE COUNT         2092 a         1358 c         1527 g         1806 t
ORIGIN      
        1 attacataac cgttggtttg cttaatatgc ggcaccttgg cagtgatatt tacacagcct
       61 gtgccacatg gcagtgtgat gttccggttg gcgatggtaa ataccgtggc cgggttttgt
      121 gctgtgacaa gcaaggccat agcacacccc gtgagggata aaaatagttt tctcatcagc
      181 agttggtcct gtcagccgac aggcaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnntatttct gcatcggtta tgttacaggt tatgggattc tatcagagat gggcaattgt
      301 tgaaaatttc aacagatagg ttaacgaggt cgccaaacgt acaaatttta tatagttaga
      361 aaaaacattt ttgcaggttt ttgccaccca taaagcatta aaagactttt aatggttcca
      421 ataaggcaaa atgacctaca tttgcaaccc caaaacagga taatagctta taagtatgag
      481 ctgttgaata accggatgtg gtgaaattgg tagacacgtc agacttagga tctgatgccg
      541 caaggtgtgg gggttcgagt ccctccatcc ggactgctta ggaaatggtt gattggtgaa
      601 ttggttttag gctggtttcc aatcaaccaa ttaactaata cccaattaac aacaatggct
      661 acagttacca aagaaaacat cggcctgctg catgaaaagc tgaccgtgaa actggaaaag
      721 accgattatc tcccctcttt tgaaaaagca ctgaaagaat acagcaagaa ggccaatatc
      781 cccggatttc gtaaaggaat ggtacctgcc ggattaatta aaaaaatgta cggtccttct
      841 ttgtttaccg atgaagtatt gaagtcggta gaccgggaac tgatcaacta tctgcaaaat
      901 gacaacctgg atatatttgc ccagcctttg ccaatggaat ctgatttccg ccagctggat
      961 gtaaataatc cagttgatta tacatttcat tttgagatag gaatgaaacc ttctttccag
     1021 ctacctgatc tgggtaaggc aaaaattact cgccatgtgg taaaggttac tgaagaaatg
     1081 atcgataatg aagttacccg tctgcagaac cggtatggca atatgaaaga tgaagacagc
     1141 atcaattcag atgataatgt gctgaatatt gttttaactg aaacagacgc tgagggtaat
     1201 gaaacggaag gcggtatcaa gaaagataat tctgttctgc ttaaatattt tgctgannnn
     1261 nnnnnnnnnt gaaaatttcc gcaataaatg gatgggcaaa aaggtaggtg atgcagaagt
     1321 ggcacaactg aaatccgctt ttgacgataa agaaagggaa tggatcatca gcgatcttgg
     1381 cttaaaagat gacatttcag cagctgataa atatttcaag atagaaatca ctaaaatcgg
     1441 catccaggaa aaaagagaac tgaatgaaga attcttcaac caattgttcc ctaaccagag
     1501 tgtggttacg gaaggcgaat tccgaagcaa agtaaaagac gagatacagg cttactggaa
     1561 cgggcaggcg atgaatcaga tacacgacca ggtatttcat gaactggttg atcataccaa
     1621 cattgaattt ccggaaggtt tcctgaaaaa atgggtaaag tcacagggtg agacccaaaa
     1681 gtcagatgaa gaggtagagg cagaattccc taaattcctc agccagttaa aatggacact
     1741 gatttctgat aaaattgttc aggacaacgg catccaggtt aaccccgacg agatcagaaa
     1801 ttttgccaaa cagcaactgt ttagttacat gggcggtgcc aacctcagcg atgaccaacc
     1861 atgggtaaat gactatgtag agaaaatgat gaaggacaga aaatatgtgg aagattctta
     1921 caatcgtatt caaactcaga aaatatttga atgggctgcc ggccaggtaa accctgcaga
     1981 taaagaaatt tcagcagaag atttcaccaa aatggtggaa gagcatcagc accaccatca
     2041 ttaattaagc ttaaaagtaa actggttaaa aggttgatta ggataataac taatcaacct
     2101 tttttttata ctgtcaaaca aaaacagtca acttgtcact gattttgtgg ttttgctatt
     2161 tggacggata tttggggtgt tcatgtttaa agtttaaagt tcaacgttgg acccccgaat
     2221 ttggaaaata tacttttccg tcagaaaact ccaacaataa aaagctttaa accttaaacc
     2281 cgtaaacttt aaacgatttt ttcatgagtt ttaattctga atttgaaaaa tacgcagtaa
     2341 aacatcacgg catttcaagt aataagctga atagttatgc ttctcatctt gtaactgcca
     2401 tgacgcctaa tatcatcgaa gaacgttccc tgaatgtggc agtgatggat gtatatagtc
     2461 gattaatgat ggaccgtatc atctttttag gctatccgat aaatgatgaa attgccaata
     2521 ttgtaacggc tcagttgctg ttccttgatt ctaccgatcg ttcaagagac atcaacatgt
     2581 acataaacag tccgggcggc agtgtatact ccgggctggg agtatatgat accatgcaat
     2641 atgtaagtcc tgacattgcc accatttgta ttggtgtagc tgcttctatg gcttgtgtgc
     2701 tgctgggtgc cggaacaaaa ggcaaacggg ctgcactgaa gcattcacgc atcatgatgc
     2761 atcagcccag cggggcaatt ggcggccagg caagcgatat tgaaatcacc gtcaatgaaa
     2821 tccgcaaact gaaaagagaa ttgtatgaag tagtgaatat tcatacaggt aaacctattg
     2881 atcagattga aaaagatttt gacagggaca actggatgac tgcttcagaa gctaaagact
     2941 acggtcttat tgatgaggta ttattaacaa acccaagaaa acaaaagaaa gactaaagaa
     3001 acttaaccct ttaaaagtat tacatgaacc gcaaacaatt tatatacaac gactggaaag
     3061 cagaagatga agaagagaag gaagaggaat ccaaaagtga catcggcatg tttggaaaaa
     3121 aattagaaaa actcttcttt gaaaaaagag ctatatatct ctggggtgtt gtggatgata
     3181 aaagcgccaa agatgtggtt agcaaattgc tgttactgga tgctgataaa cccggagaag
     3241 aaattaagtt tttcattaat agcccgggcg gtgtggttac cagtggcatg gtaatgtatg
     3301 ataccatgca aatgattaaa agccccgtga gtacgatatg catgggcctc gcagcatcta
     3361 tgggaagcat cctgctgagc ggcggtgtaa aagggaaacg ctacatctac ccccatggtg
     3421 aggtaatgat acaccagcct tctttaggcg gatatataca gggagtaagt gccgacctgg
     3481 aaattcaggc ccgtcaaacc ttgcgggtaa aagagatcgg tgcgtcgatt ttggcaaaaa
     3541 actgcggcaa aacagttgat cagatcatga aagattttgc ccgtgactac tggatggatg
     3601 ccaaacaagc cattgaatat ggtatagttg acgggattgt agagagcttg taacaggctg
     3661 aactcaagca tatatatggc ccacttgcag gtttttgcaa ggtgcatata aaataaaacc
     3721 gcctcaatcg gggcggtttt ataattttgt gagttacgaa ttcgttggta attcgtttta
     3781 ccttataaaa aatggcaaaa aatccactac actgttcttt ttgcggccgc agccgtgatg
     3841 aagtaaagat tctcatcgcc ggccaggaag gacatatttg tgaaaactgc gtagaacatg
     3901 cccgggagat cattgaccag gaactggcca ttaaagacga ccgcagtaat tcttccttca
     3961 aactcacagt taagaaaccc gttgaaataa agaaatttct cgacgaatac gccatcggcc
     4021 aggatgaagc caaaaaagtg ctggcggtag ctgtttacaa tcactataaa cgattacagc
     4081 aaaaaacggc cgataacggt aatgatattg aaattgaaaa gagcaatatc ataatggtgg
     4141 gagaaactgg tacaggtaaa actttattag caaaaagcat tgcccggatg cttaatgtac
     4201 catttgcgat tgttgatgca acggtattta ccgaagccgg ttatgtgggc gaagacgttg
     4261 aaagtattct taccaggtta ttacaggtat gtaactatga tgtagctgcc gctgaacggg
     4321 gcattgtata tattgacgag attgataaaa tagcccgcaa gggtgacaac ccgtctatta
     4381 cccgtgatgt tagcggtgaa ggtgtgcagc agggaatgct aaaactgctg gaaggaacag
     4441 atgtgctggt accaccacag ggcggacgca aacacccgga gcaaaagctt atcaagatca
     4501 acacacagaa tattctcttc atctgcggtg gcgcatttga cggcattgac aagatcattg
     4561 cccgcagggt acaaaccaac accattggtt ttaatgtgga caaggaaatg caggaaacca
     4621 tgaagaaaaa cctgttgcgt tatgtaaatg ctaccgacct gaaggcattt ggtcttatcc
     4681 ctgaattgct gggacgatta ccggtggtaa cacatcttga tccgctggat gcaactaccc
     4741 tgcgagccat tttgactgaa cccaaaaacg cattggttaa gcagtacaag aaattatttg
     4801 aactggaagg catacaactc accattgaag aagatgtgct tgacttcatg gtagaaaaag
     4861 ccatggaata caaactcgga gcaagaggcc tgagaagtat ttgcgaagga atattgattg
     4921 atgcgatgta tgagcttccg tcatcaaaag aaacccagtt taccctcgac ctggaatatg
     4981 ccaaacgcaa atttgataag agcaagctga gtttgttgaa agtggcgtaa gtaccttcgt
     5041 tgatcggatt taaaaagata attaggatat ttggtcagac aatttagtct gaccatttct
     5101 tttaatataa tactatgcaa gaattaattg aaaaactgaa aaccgaagca ggacttactg
     5161 atgagcaggc caaacaagcc attgccacga taaaaaattt cgtagtagag aaattcccta
     5221 tgnnnnnnnn nnnnnnnnnn ngtgcggtta acaatatttt cggggcagaa taaccgcttg
     5281 tcacccttcg acaagctcag ggtgacaaag cctcatttta tatctctgca atgctttccc
     5341 cgtgggttat tggcggcctc atagggactc atagccctgt tacaggcctg gaataaaaaa
     5401 ctggctaaca gcaaaaccat aacgatcctt gtagcattct tcatgggtat cagattttgg
     5461 ttgtaaaata aaaaagctgc tgataaaaca agaagggaat tactactgaa aacttctttt
     5521 ttcggatagc atttttacct aataaaacat cccggctcaa tggccgggat ggaattaatg
     5581 ggtactttct ttatttggtt tttctaaaaa tccgccgata ttactagtaa aggcggaccg
     5641 tccttgacaa tttaaccaca acctccctgg ttgccacagt tcaggcattt gtagcaggca
     5701 ccactgcgca tcatgatatt gccgcataca ttacaggcag gtgcatcact ctgcatgccc
     5761 ttcaatgctt cattcatagc gccggttccc gcatccgcct ttaccgtttg tgctgtacgc
     5821 tggtttctta ccggctgggg agctaccgtt cccgatgaag gagtaatcct tacactggat
     5881 aattcagggt cctttgcata ttccagtccg ttggagggga tctcatccca gtcatctgaa
     5941 ccggtattca tcacttcggg tttatccaat acatgtacca ggtcggtgcg gcccaggtat
     6001 tcatatccga gtacacggaa gatgaaatct actattgaag tagttgtcct gatgttcggg
     6061 tgttctacaa atccggatgg ttcgaaacgg gtaaaggcaa atttatcaat gaactcttcc
     6121 agcggtacac catattgcaa accgattgag atagaaatgg caaagcagtt catcatgctg
     6181 cgcatcgtag ctccttcttt tgccatatca ataaatatct ctcccagcgt accatcactg
     6241 tattcaccgg tacggaggaa tacagcctga ccgttgatct ttgctttttg tacaaagccg
     6301 cggcgcttgg caggcaatgt tcttctttct acaatgctgg ccagtttacg tttcagttgc
     6361 gtatcaggtg aagcctgtac ccttttctgt acttcttcca gcagctcttc tacagttaat
     6421 ttgcccagat caacgatatt cgattcgttg gccgacgcca gttgctcgtt ggctgcggac
     6481 tgctgactgt caactgttga tttttcatct ccggttttct tcttcttatc actcttatta
     6541 ctgagcggtt gactcagttt agaaccatct ctgtaaaggg cattggcttt caggcccagc
     6601 ttccagctca gatgatacgc ttctgctatc tcccccactt ctgcttcatg cggcaggttg
     6661 atagtcttgg aaatggcacc gctgataaag ggctgtacag ctcccatcat acggatatga
     6721 ccatgggcat ggatataacg ctggcctttc tggccgcatt tgttggcaca gtcaaaaacg
     6781 ggcaggtgtt cttctttcag ccccggtgca ccttccagca tcatagtacc gcatacataa
     6841 tcattggcgg c
//
LOCUS       DAORLS010000159         6847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_5978_length_6847_cov_2.020024, whole genome shotgun sequence.
ACCESSION   DAORLS010000159 DAORLS010000000
VERSION     DAORLS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_6847_cov_2.020024"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..>2285
                     /locus_tag="PK977_12070"
     CDS             304..>2285
                     /locus_tag="PK977_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF18900.1"
                     /translation="MRKTMMLMLTVLVLSVQSLLAQNKTLTGKVTDNNGAPIAGASVM
                     EKGTNNGTFTGQDGTFSLSVSDKAKALVVSAVGFTSQELIISGTTFQVSMKSGEAQSL
                     DEVVVVGFGTKIKKDLTGNIARVKGSEVANTPVPNFNQALQGRAAGVFVESNNGKVGE
                     GVKVRIRGAGSLSASNSPLYVVDGVPLNGDGISGNSLADINFNEVESFEILKDASAAA
                     IYGSRAANGVVLITTKKGKAGKTTLNVNAQYGVNKPTGYRGFLNAKEYVDLLREAAIN
                     SDNLEGVDPLDPAQYPGSWLQFAENRLTRYSGYSNWRNLETNTDWEKQAFNDESRTKI
                     IDINASGGNDKTRFFVSGGYTNQDGILVGNNFQRFSGRINLEHDASNKLKLGFNLGLS
                     RSVGNRVALDNEFSTPLQLVALAPITPIRDLNGNLYDRPVTTYYNGLIELENSSYVST
                     TYRNIGSLFLNYKIFTDLTFRTEFGIDLQTQNDEQFYGSRTINGQSTNGYAQSDWFRT
                     FNYNTNNYFNYVKRFGGIHNIDATLGMSYQKYTTNFTNAFGEDFPVDALRTLASAGRI
                     TGGTSTLNESSFLSYFARANYKLNEKYLLTLSGRIDGSSRFGKDNKYGFFPAASLGWI
                     LSEEEFLKSVNWLSFLKLRGSFGLTGNADGFGNFAQL"
     assembly_gap    2286..2383
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2384..3355
                     /locus_tag="PK977_12075"
     CDS             <2384..3355
                     /locus_tag="PK977_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF18901.1"
                     /translation="QVDIGIDFGLFNNRLTGEIDYYVRNTNDLIYNVPVPGTSGFATQ
                     TVNVGSMQNKGVEFVLNSTNFNGRDFKWNTSFNLSKNKNKITKLDGEQTTIPGNDGRY
                     LNSLIVGQPIGVFYGPKFAGADPANGDALYYKEDGKTTTNDYNEAGSFVVGDPNPDWI
                     GGLNNTFSYKGFELNVLLQGVFGNQIQNGAGGFMSASFDWFDNQTRDQLNRWQKPGDV
                     TNVPQLRLGYGNGIGASSRYVEDGGYVRLKNVTLAYNIPAKLLTKLKIRSARFYVTGI
                     NLATFTDYTGWDPEVNTDYRAGNRNQGSDFYAAPQIKSVTFGVNLGF"
     gene            3373..4719
                     /locus_tag="PK977_12080"
     CDS             3373..4719
                     /locus_tag="PK977_12080"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF18902.1"
                     /translation="MKRNIIAITLLSVIVASCGKKLDLLPQQSVAEEVALNSDANVKK
                     VLNGAYDAVSDGDLYGGNLMLFAELLAANDEIRWEGTFNQPREVWLKAMLTTNAYVRD
                     TWLEAYDVINICNNIISAIGVVNSADQNRVKGEALFLRGAMYFELVKLFAKPYSAGNV
                     STNKGLPLILAPTRGIDQSSYVQRSTVEQTYTQIIADLTEAESLLPNNNGVYASKYAA
                     AGMLSRVYLQMENYPSARDAADRAITVATGAGKSLAGTYAAAFNNSSNSAEYLFAMQV
                     SAQDGTNNMHLFWSIPAYGGRDGDVSIQASHLALYTASDARLALFYTGAGDVRSGKWQ
                     QQYRILPIMRLAEMYLTRAEANFRAGTSVGATPAQDVSRTRQRAGLSAVGTVTLAEIL
                     AERKLELAHEGQGVHDLKRLKQSADGFAYDANEMVFPIPQREVDASRGVIAQNPGY"
     gene            complement(4804..6357)
                     /locus_tag="PK977_12085"
     CDS             complement(4804..6357)
                     /locus_tag="PK977_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRF18903.1"
                     /translation="MNKELIINAAPQGVEIALLEDKKLVELHSEKSDARFAVGDLYLG
                     KVKKLIPGLNAAFVDVGFEKDAFLHYTDLSPYAKSLLKFTTMCMNDKSEQGLDFSKFT
                     IEPEIVKTGKINEVLGGKPNILVQILKEPIAAKGPRLSCEISLPGRFVVLTPFNDIVA
                     VSRKIHSSEERKRLQKIVEAIKSKNFGVIVRTAAEGKNTAELHEDLSALVEMWKAIQQ
                     NLKGAVAPTRILSEQTKTTSILRDLLNADFNRIVVNDKNIYSDTKNYIQRIAPEKAEI
                     VSFYNNGSPIFDSFGITKQVKSSFGKTVNLDSGAYLIIEHTEALHVIDVNSGYKSVSN
                     NQEQNALETNLEAAEEIARQLRLRDLGGIIVVDFIDMKLPENKRKLMEKMEEFMSPDR
                     AKHAVLPISKFGIMQITRQRMKPEVNINTQEVCPSCNGTGKISSTLLLEDEIEKNLSY
                     LVTHKNANLKLVVHPILYAYLTKGWPWNTKMSQWKRKYGQKTRLEKDSNYHLTEYKFF
                     DKNDEEIKF"
     assembly_gap    6501..6621
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1832 a         1368 c         1463 g         1965 t
ORIGIN      
        1 caagttgaaa aactggtaaa aacgcaggcg ggaacaattc ttattgcgaa tgtttcatta
       61 acaatcggct gatccggatt tctacggacg taccgggacc tgcagcacgg gaacctctcc
      121 ggagctttgg ccgctgtggt aaccctgatt caatatataa tgctttacct gggcagcagg
      181 gatagaagtt agcctgactt tttgaccaga atcaatattt agagccgtgc agcaacctgc
      241 atggtttttt ttgtccttaa gaaatcgtta aatattttcg ccgacattaa aaaaatcaca
      301 cacatgagaa aaactatgat gcttatgttg acggtgcttg tgttatcggt ccaatcccta
      361 ttggcccaaa acaagacgtt gacaggaaaa gtaacagaca acaacggggc gcctattgcc
      421 ggagcttccg ttatggaaaa aggaactaac aatggaacat tcaccggcca ggatggtact
      481 tttagcttat ctgtttcgga taaagcgaaa gccctggttg tgtctgctgt cggtttcaca
      541 tcacaggagc tgattatttc cggtaccaca ttccaggttt caatgaaatc gggtgaagca
      601 caaagcctgg atgaagtagt tgtagtgggt tttggtacta agatcaaaaa agaccttact
      661 ggtaatattg cccgggtaaa aggatctgaa gtagctaaca caccagttcc caattttaac
      721 caggccctgc agggtagagc cgcaggtgtt tttgtggaat ctaataatgg taaagtgggt
      781 gaaggtgtga aagtaagaat caggggtgct ggttctttaa gtgcatctaa ctctcctttg
      841 tatgtagtag atggtgttcc actaaacggt gatgggatat ccggcaattc tcttgctgat
      901 ataaatttta atgaagtaga gtcatttgaa attttaaaag atgcctctgc tgctgcaatc
      961 tatggatccc gtgctgccaa tggtgtagta ttgattacaa ccaaaaaagg taaagctggt
     1021 aaaacaacac tgaatgttaa tgcacaatat ggtgtaaaca agccaaccgg ttatagaggt
     1081 ttccttaatg caaaagagta tgttgacctg ttaagagaag ctgctattaa cagtgataac
     1141 ctggaaggtg ttgaccccct ggatcctgca caatatcctg gttcatggct tcagtttgca
     1201 gagaaccggc tcacccgtta ctctggttac tctaactggc gcaacctgga aacaaatact
     1261 gactgggaaa agcaagcatt caacgatgaa tcccgtacaa agattattga tatcaatgcc
     1321 tctggcggta atgataaaac aaggttcttt gtaagcggtg gctatactaa ccaggacggt
     1381 attcttgttg gtaataattt tcagcgtttt tcaggtcgta tcaacctgga gcatgatgca
     1441 agtaataaac ttaaactagg gttcaacctt ggtctttcaa gaagtgtagg taacagggtg
     1501 gccctggata atgaattttc tacgccgttg caattagtgg ccctggcgcc aattacaccg
     1561 attagggatc ttaatggcaa tctatacgac aggccggtta ctacttatta taacggcctg
     1621 atcgaattag agaactcaag ttatgtatct actacctaca gaaatattgg tagcctcttt
     1681 ctgaactaca agatattcac tgatcttact ttcagaactg aatttggcat tgatctgcaa
     1741 actcaaaatg atgagcagtt ttatggttcc agaaccatca atgggcagtc tacaaatggt
     1801 tatgcccagt ctgattggtt caggacattc aactataata ccaacaacta tttcaattat
     1861 gtaaaacgtt ttggtggtat tcataacata gatgccacat taggtatgtc atatcagaag
     1921 tatacaacca atttcactaa tgcttttggt gaagactttc ctgtagatgc acttcgtact
     1981 ctggctagtg ccggacgaat taccggaggt acttccactc tcaatgaatc atcgttttta
     2041 tcttatttcg caagggccaa ctataagctt aacgaaaaat acctgttaac cctgagtggc
     2101 cgtatcgatg gttcttccag gtttggtaag gacaataaat atggtttctt cccagcagct
     2161 tccctgggat ggatattgag tgaagaagaa tttctgaagt cagtaaactg gttaagcttt
     2221 cttaagctgc gtggtagttt tggtcttact ggtaatgctg atggtttcgg aaactttgct
     2281 cagctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaggtag atattggtat
     2401 tgactttggt ctgtttaaca acaggcttac tggtgaaatt gattattatg taagaaatac
     2461 caatgacctt atttacaatg tacctgttcc cggtacttct ggttttgcta cacaaacagt
     2521 aaacgttggt tctatgcaga ataagggtgt tgaatttgtt cttaattcaa ccaacttcaa
     2581 tggaagggat tttaagtgga ataccagctt taacctttct aagaacaaga ataagatcac
     2641 aaaactggat ggtgagcaaa ctaccattcc tggtaatgat ggacgttacc tgaactcact
     2701 catcgttgga cagcccattg gtgttttcta tggtcctaag tttgcaggtg cagatcctgc
     2761 aaatggtgat gcactttatt ataaagaaga tggcaaaaca accaccaacg actataatga
     2821 agccggtagc tttgttgtag gtgatcccaa cccggattgg atcggtggtt tgaacaacac
     2881 atttagctat aaaggttttg aactgaacgt actgctgcag ggtgtatttg gcaaccaaat
     2941 acagaatgga gccggtggat ttatgtcagc cagtttcgac tggttcgata accagacccg
     3001 tgaccagtta aaccgctggc aaaagcccgg tgatgttact aacgtacccc agttaagact
     3061 tggttatgga aatggtatcg gtgcttccag ccgctatgtg gaagatggcg gttatgttcg
     3121 tctcaaaaat gtaacgctgg cctataatat ccctgccaaa ttgctgacta aattaaagat
     3181 ccgttctgca agattctatg ttacaggtat caatctggcc acatttaccg attataccgg
     3241 ctgggatcct gaagtaaata ccgattaccg tgccggtaac cgtaaccagg gcagcgactt
     3301 ctacgctgct ccgcagatca agtctgtgac atttggcgta aacctcggat tttaattttt
     3361 aaaatgaaaa aaatgaaacg taatataatt gcaataacac ttctttcagt aattgttgct
     3421 tcttgcggca agaaactgga ccttcttccg cagcaaagtg ttgcagaaga agtggcattg
     3481 aactctgatg ccaatgttaa aaaggtcctt aacggtgcct atgacgctgt aagtgatggt
     3541 gatctctatg gtggtaacct tatgcttttt gctgaactgc tggcagctaa tgatgagatc
     3601 aggtgggaag gaacattcaa tcaaccccgt gaggtttggt tgaaagccat gcttacaact
     3661 aatgcatatg taagagatac ctggctggag gcatatgatg taattaatat ctgtaacaat
     3721 atcatcagtg ccattggagt agtgaatagt gctgaccaga acagggtgaa aggcgaagcc
     3781 ctatttctca gaggagctat gtactttgag ttggtaaagc tttttgccaa accatacagt
     3841 gcaggtaatg taagtacgaa caagggcctt ccgttgattc tggctccgac aagaggtatt
     3901 gatcagtcat cttatgtaca acgcagcacc gtagagcaga catatacaca gataattgcc
     3961 gaccttactg aggccgaaag tcttttgcct aataacaacg gagtgtatgc cagcaagtac
     4021 gcagcggcag gcatgctgtc ccgtgtctac cttcagatgg agaactatcc atcagcaaga
     4081 gatgcagctg accgtgctat tacagtagct actggtgcag gaaaaagcct tgcaggtacc
     4141 tatgcagcag cttttaacaa tagcagcaat tcagcagagt atcttttcgc tatgcaggta
     4201 tctgcacagg atggaacaaa taacatgcac ctgttctggt ccatacctgc ctatggagga
     4261 cgtgatggtg atgtttcaat acaagcaagt catctggcac tttatactgc ttctgatgca
     4321 aggctggcat tgttctatac cggagccggc gatgtgcgca gcggtaaatg gcaacagcag
     4381 tacagaatac ttccaataat gcgtttggct gaaatgtacc tgacccgtgc tgaagctaac
     4441 ttcagagccg gtacttctgt tggtgctaca cctgcacagg atgtaagccg aacaagacaa
     4501 agagccggtc tttctgccgt aggcactgtt acgctggctg aaattcttgc ggaaagaaaa
     4561 ttagaactgg cacacgaagg acagggagta catgacctta agcgtttaaa acaaagtgca
     4621 gatggctttg catatgatgc taatgaaatg gtatttccaa ttcctcaaag agaagttgat
     4681 gcatcccggg gagttattgc tcaaaaccca gggtactaag agttatattt ctgcttagaa
     4741 caaaatgaaa gccccgtctg atgacggggc tttcttatga tgtaatttta gaaaagagtt
     4801 ttattaaaac tttatctctt catcgttttt atcgaagaat ttgtattcgg taaggtggta
     4861 gttactgtct ttttccagcc ttgttttctg accatacttt cgcttccact gcgacatttt
     4921 tgtattccag ggccaaccct ttgtcaggta agcgtataaa atgggatgta caaccagttt
     4981 aaggttggca tttttatgtg ttaccaggta acttaagttc ttttctattt catcttccag
     5041 cagcagggta gaagaaatct ttccggtgcc attacagctt ggacacactt cctgagtatt
     5101 gatatttact tctggcttca tccgctggcg ggtgatctgc atgatgccga atttggaaat
     5161 aggaagcaca gcatgcttgg ccctgtctgg tgacatgaat tcttccatct tttccatcag
     5221 cttccgctta ttttccggca gcttcatgtc aataaaatca accacaatga tgccgccaag
     5281 atcccgcagg cgcaattgcc tggcaatttc ttccgctgct tcaaggtttg tttccagggc
     5341 attttgctcc tggttattgc ttacactttt gtaaccgctg ttcacatcta ttacatgcaa
     5401 agcttcggta tgctcaatga taaggtaagc cccgctatca aggtttactg tttttccaaa
     5461 agaagatttc acctgcttgg tgataccaaa tgaatcaaaa atgggtgaac cgttattgta
     5521 aaacgaaacg atctctgctt tttcaggtgc aatgcgttga atataattct tggtgtcgct
     5581 gtaaatattt ttatcgttca ctacaatgcg gttgaaatca gcatttagca agtcacgcag
     5641 tatactggta gttttagttt gttcactcag tatccgtgtg ggtgctactg cccctttcag
     5701 gttttgctga atggccttcc acatttctac aagggccgaa agatcttcat gcagttcggc
     5761 tgtattcttt ccttctgcag ccgtacgtac aatgacacca aagtttttac tcttgatcgc
     5821 ttccacaatc ttttgcaaac gtttcctttc ctctgatgag tggatctttc tcgatactgc
     5881 tacaatatca ttgaagggag taagtaccac gaaacggcca ggcaaggata tttcacagct
     5941 aagcctgggc cctttggcag caataggttc tttcaaaatc tgcaccaata tgttgggctt
     6001 gcccccgagc acttcattga ttttaccggt ttttacaatt tccggttcaa tagtgaactt
     6061 ggaaaaatca agaccttgct cactcttatc attcatacac atggtagtga acttcaggag
     6121 cgatttggcg taagggctca gatcggtata gtgcaggaaa gcatccttct caaacccaac
     6181 atccacgaag gcagcattca gcccgggaat cagtttcttg acttttccca ggtacaagtc
     6241 gcccacagca aaacgggcat cgcttttttc gctatgcagt tctaccagtt tcttgtcttc
     6301 cagcaaggca atttcaacgc cttgcggagc cgcattgata ataagttctt tgttcattac
     6361 agcttacata aatgaatcac cttagtgctg taaacaccag ttgccaatat tccgtaagga
     6421 atgcaggttg tatctgcgga tattttgcgg aggagtgggt gagatgctgg gagttcagga
     6481 cgggattgac ctgtattttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nctttttacg cttgtgcgtc gcaattttat gacgcttcct
     6661 ttttttacca caaggcatac tcaaataatt ttttaatgtt ctgtttataa taagtttcgc
     6721 aacttctatt tcttcaactc ttcaattctt ttttgaagag ctgttttcag ctctgcctca
     6781 ctcaccaggt tcaggcattg ctggtaccat ttcaccgcac tcttcttatc accggtacgt
     6841 tcatagg
//
LOCUS       DAORLS010000160         6817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6016_length_6817_cov_2.236321, whole genome shotgun sequence.
ACCESSION   DAORLS010000160 DAORLS010000000
VERSION     DAORLS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6016_length_6817_cov_2.236321"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..>798
                     /locus_tag="PK977_12090"
     CDS             265..>798
                     /locus_tag="PK977_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRF18904.1"
                     /translation="MPGSFNSQGWINWLEVFARRNISLNGNESLFFRDWNSVGINNGE
                     FVVRNATVSTQVWDITDFNNPVRIQGTFSNSEFRFVNTCERLREYVAFNTTTGLFKTP
                     VSAGKISNQDLHGAPAADFLIITHPLFFSQSQRLAQLHQQNDNLRSLVVTTEQVYHEF
                     SSGSPDPVAIRDFVKMFY"
     assembly_gap    799..808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <809..2845
                     /gene="porU"
                     /locus_tag="PK977_12095"
     CDS             <809..2845
                     /gene="porU"
                     /locus_tag="PK977_12095"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRF18905.1"
                     /translation="DKAGSNQVNRPKYLLLFGDASYDYKDRLVNNTNLVPAWQNNFSL
                     DPLSTYASDDFFGFLDDNEDINSGLVTNLLDIGIGRVPAKNAEEAKNFVDKVAVYLSP
                     QSLGSWRNNLTFIADDEDNNLHLQDAEIITGTAGNVGPVFDQQKIYLDAFLQESGAGG
                     SRYPQANEASNNQVYNGTLIWNYNGHGGSSRLAEENILDQPTVNNWNNPERLPLFITA
                     TCDFAPYDNPFVNSLGENILLRPKTGAIALMTTTRLVFAFSNRIINNNYLQFALQPDA
                     SGKYRSLGDAVKDAKNFTYQNSGDIANNRKFTLLGDPALTLAFPKMKSRISRVNGIDI
                     SIADTLNALEKITMEGEITDWQGNLLSDFNGNVYPSVFDKPKFINTLGNDPGSQVTSF
                     TSQGNFLFKGKATVTNGRFSFTFKVPKDINYQFGNGRVSLYAENGISDASGFLTNLVV
                     GGSSSGTDADKEGPRIKAYLNDELFVNGGITNQNPVLIANLADTSGINTAGTGIGHDI
                     IATLDNDNNRYFILNDFYQGELNSYQQGTVRFQLPVLDPGPHTLKIKAWDVVNNSNEV
                     LLDFIVVNDTELELNHVLNYPNPFTNATTFWFEHNKPGLPLAVQLQVMTVTGRVIKTL
                     EQVVITEGNRSADIRWDGRDEYGDKPGRGVYLYKLRVIAPGRKQKEVLGKLVML"
     gene            3175..4329
                     /gene="porV"
                     /locus_tag="PK977_12100"
     CDS             3175..4329
                     /gene="porV"
                     /locus_tag="PK977_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRF18906.1"
                     /translation="MRPTALKLTAGFLLLCGLSSTVKAQVEPLNVVTTAVPFLRISPD
                     ARSGGMGDLGVATSPDAYSGLWNVGKVAFNTGKGGVGATYTPWLKDLVNDVYLASLAG
                     YYKFADNQAIHGAVRYFSLGNITFTDNLGNDFGSFRPREFGIDIGYSRKLNAKNGIGI
                     TLKYINSNLAGNTTVGSTTYKAGNAVAADLGWFHNGVGATGSGWNWGVVLSNLGSKIS
                     YTDNADAKDFIPANLGFGTNYTKKFNAQNKISFGVELNKLLVPTPPADPATAQELADY
                     RSKSVIGSWFSSFGDAPDGFGEEIKEFQASLGAEYTYNDQFMLRAGYFYENKTKGNRR
                     YFTAGLGVKYNIFGFNFAYLVPTGSGVSRNPLSNTLRFSILFDFNGPAAK"
     gene            4412..4894
                     /gene="ispF"
                     /locus_tag="PK977_12105"
     CDS             4412..4894
                     /gene="ispF"
                     /locus_tag="PK977_12105"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRF18907.1"
                     /translation="MSFRIGSGVDFHQLTEGRELWIGGVKIPHHKGALGHSDADVLLH
                     AICDALLGALSLGDIGLHFPNNDPAYKNIDSKLLLKKTIEMVRDKGYRIVNIDSTLCL
                     QEPKIKPYVSAMQKVIADISGIQTSDVSVKATTTENMGFVGREEGLMAYATVLLERKA
                     "
     gene            4939..5382
                     /gene="dut"
                     /locus_tag="PK977_12110"
     CDS             4939..5382
                     /gene="dut"
                     /locus_tag="PK977_12110"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRF18908.1"
                     /translation="MPAIEINVINKSTNPLPEYATAGSSGMDLRASLQEPVSFRPLQR
                     LLIPTGLFIELPAGYEAQVRPRSGLAIKQGITCLNTPGTIDADYRGEIKIILINLSGE
                     EQVINHGDRIAQLVIQKVEKACWKETDELAITARNEGGFGHTGHQ"
     gene            5394..6212
                     /locus_tag="PK977_12115"
     CDS             5394..6212
                     /locus_tag="PK977_12115"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRF18909.1"
                     /translation="MNQLSVSLIAITCFLYACRPARNIQTTPPVADTISIAVTPTDNP
                     KEDSARVIKENYDRILAGQIQFTTFNAKIDVEYRDADGKDINANAHLRMYKDSVIWLS
                     LTGPLGIEGIRAYITKDSVKLLNKQDKIYTARSVSYLQEVTELPLDLSSLQNLLAGNP
                     IFFDSTITGYSLSPGSISLFSAGEFFNNLLTIGESDKYLQSCKLNDLDSTRARTCYLT
                     YSAYENKRGYNFSTRRSITVTDKKKLDIELDFRQYDFNETLSFPFSIPKNYKRN"
     gene            6294..>6817
                     /locus_tag="PK977_12120"
     CDS             6294..>6817
                     /locus_tag="PK977_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18910.1"
                     /translation="MRKISLLIFFILFAGITVFAQDKTQLEKERQQIQKELQEIQTLY
                     NKVKGQTKQTLGQLNVLNRKISLYEQYISSINKEIKSIDDDIYLSNLEIYRLQKQLDT
                     LKTQYAKTIVYAYKNRSSYDYLNFIFSANSFNDAVKRISYLKSYKDYRERQVSTILET
                     QQLIAARKKQQLSR"
BASE COUNT         2069 a         1325 c         1431 g         1982 t
ORIGIN      
        1 ttgccaggga gatcactatc aaggaatttc tctttcaata ttatcggcct tgttcaaaat
       61 tcagctcttg ccttaaagac agagtgtata gctagatctt caggggtttc cagccggttt
      121 gatattgcta ttaataacca accggctggt caaataacta tcccccagat tggtagtggt
      181 ttatatgatc tttttgcaca gtcatcttca tcggttttaa gtgccaatgt cctatcggaa
      241 gcagttacta taaactatac ttatgtgccc ggaagtttta attcgcaggg atggatcaac
      301 tggctggaag tttttgcccg cagaaatatt agtctcaatg gcaatgagtc actgtttttc
      361 cgggactgga acagtgtagg aattaataat ggtgaatttg ttgtaaggaa tgccacagtc
      421 tctactcaag tttgggatat tacggatttc aataaccccg ttcgtataca gggaactttt
      481 tctaatagtg aattcaggtt tgtaaatacc tgcgaaagat taagagagta tgttgctttt
      541 aataccacaa ccggtttgtt taaaacaccg gtctctgcag ggaagatcag caaccaggat
      601 cttcacggcg cacctgctgc agatttttta atcatcacac atccgctttt ttttagccag
      661 tcacaacggc ttgcccaatt acaccagcaa aatgataatt tacgttcatt ggttgttaca
      721 acggaacagg tctaccacga attttcttcc ggtagtcctg atccggtggc tatccgggat
      781 tttgtaaaaa tgttttatnn nnnnnnnnga caaagcagga tccaaccagg taaacaggcc
      841 taaatattta ttgctttttg gggatgcttc ttatgattat aaagacaggt tggtcaataa
      901 taccaacctg gttccggcat ggcagaacaa tttttcatta gatcctcttt ctacttatgc
      961 atctgatgat ttttttggct ttttggatga caatgaagat atcaattcgg gcctggtaac
     1021 taaccttctg gatattggta ttggcagagt tcctgcaaag aatgcagaag aggctaaaaa
     1081 tttcgttgat aaagtggcgg tctatctttc accacaaagt cttggctcgt ggcgaaataa
     1141 tctcactttt attgctgatg atgaggacaa caaccttcat ctgcaggatg cagagatcat
     1201 taccggcact gccggtaatg taggtcctgt ttttgaccag caaaagattt acctggatgc
     1261 ttttttacaa gaaagtggtg ctgggggcag tcgctatcct caggcaaatg aagccagtaa
     1321 caaccaggtg tacaacggca cattaatatg gaattataat ggtcatgggg gatcttcccg
     1381 tttggcagaa gaaaatatcc tggatcagcc gacagtaaat aactggaata atccggagcg
     1441 gttgcccttg tttatcactg ctacctgtga ttttgcacct tacgataacc cgtttgtcaa
     1501 ttcattaggc gaaaacatac ttttaaggcc aaaaacagga gctatcgcat taatgacaac
     1561 tacccggctg gtgtttgctt ttagtaacag gattatcaat aataattacc tgcaatttgc
     1621 tttacaaccg gatgccagtg gaaaataccg gtcattgggt gatgccgtaa aagatgccaa
     1681 aaattttacc taccagaatt ctggcgacat tgccaataac aggaaattta ccttactggg
     1741 agacccggct ttgacgttag catttcctaa aatgaagtca agaatttccc gggtgaacgg
     1801 aattgatata tcaattgccg ataccttaaa tgccctggag aaaataacga tggaaggtga
     1861 gattaccgac tggcagggta atctattaag tgattttaat ggcaatgtat acccatctgt
     1921 ttttgacaaa cccaaattca ttaatacgct tggtaatgac cccggcagcc aggttacttc
     1981 gtttacttca cagggtaact ttttgtttaa ggggaaggct acggtaacca atggccgctt
     2041 ttctttcact tttaaagtac caaaagatat caactaccag tttgggaatg gaagggtaag
     2101 tctatatgca gaaaacggaa tatctgatgc cagtggattt ttaaccaacc ttgttgtagg
     2161 tggaagtagt agcggaacag atgctgataa agaaggtccc cgaataaaag catacttaaa
     2221 cgatgagcta ttcgtaaatg gaggaataac caatcagaat ccggttttga ttgctaatct
     2281 ggccgataca tccggaatca atacggcagg tacaggaatc gggcatgata ttattgccac
     2341 attagacaat gacaataacc gttattttat tctcaatgat ttctaccagg gtgaacttaa
     2401 tagttaccag cagggcacgg tccgttttca acttcctgtc ctcgatcccg gcccccatac
     2461 attaaaaata aaggcgtggg atgtagtgaa taattcaaac gaggttttgc tggattttat
     2521 agtggtaaat gatactgaat tggagcttaa tcatgtgctt aattatccca acccttttac
     2581 aaatgctacc acattctggt ttgaacataa caaacccggc ctgcctttag ctgttcaatt
     2641 gcaggttatg actgttaccg gaagagtaat aaaaacattg gaacaggtag tgattacaga
     2701 gggaaacagg tcagcggata ttagatggga tggcagagac gaatatgggg acaaaccggg
     2761 aagaggggtg tatttgtaca aattgcgggt tattgcgccc ggcaggaagc aaaaagaggt
     2821 gttgggtaaa ttagttatgc tctgaagaga aacttgcaaa atgtgattaa tcagaattat
     2881 ttgaggtttg ggcagcgaaa tcataaaata aattgtcttc ggattcgaaa ccgaaaacct
     2941 gttgaccggg catgtaggaa tgattttcgt cagctaaaat aaaatttgaa caataaaagc
     3001 cggaaagaat acgttttttc tcgaaatttg cgaccaaatc cggagctgcg attatcgcta
     3061 caaagcccca aagccattaa aaaccagttt tttttggttt cggataaaat tctctatttt
     3121 agttttgtat ttaatcaaaa gctctatatt tacaccatta ttaaaaactt actaatgaga
     3181 ccaactgctt taaaactaac tgccggattc ctgctcttat gtggtttatc ttccacagta
     3241 aaagctcagg ttgaacccct aaacgttgtt actaccgccg ttccttttct gcgtatttca
     3301 cccgatgccc gttcaggtgg tatgggagat cttggcgtag ccacttctcc tgatgcttat
     3361 tctggattgt ggaatgtggg taaagtagct ttcaatacag gtaaaggagg tgttggtgca
     3421 acttacactc catggttgaa agacctggta aatgatgttt accttgcttc acttgcagga
     3481 tattataaat ttgctgacaa tcaggccatt catggtgctg ttcgttattt cagcctcggt
     3541 aatatcacat ttaccgataa cctgggtaac gatttcggaa gcttccgccc ccgtgaattc
     3601 ggtattgata tcggatattc ccgtaagctg aatgcaaaaa atggtattgg tattacttta
     3661 aagtatatca actctaatct tgctggaaat actacagttg gtagcacaac ttacaaagct
     3721 ggtaatgctg ttgctgctga cctcggttgg ttccacaatg gtgttggtgc tacaggaagt
     3781 ggctggaact ggggtgttgt tttatctaac ctcggctcta agatctctta tactgataat
     3841 gctgatgcaa aagatttcat tcctgctaat ctcggtttcg gtactaacta tactaaaaaa
     3901 ttcaatgctc agaataaaat ttcattcggc gtagaattga acaaactgtt ggtgcctaca
     3961 cctcctgctg atcctgcaac tgcacaggaa ttggctgatt acagaagcaa gagtgtaatc
     4021 ggtagctggt tcagttcttt cggtgatgct cctgatggat ttggcgaaga aatcaaagaa
     4081 ttccaggcat ctctgggcgc tgaatatacg tacaacgacc agttcatgct ccgtgcaggt
     4141 tatttctacg agaacaaaac aaaaggtaac cgtcgttact tcacagctgg tctgggtgtt
     4201 aaatacaaca ttttcggttt caactttgct tacctggttc ctacaggaag tggtgttagc
     4261 cgcaatccat tgtcaaacac actgcgtttc tctattctgt ttgatttcaa cggacctgct
     4321 gccaaataat atatatagct gattatatac taagcgtcct gccaaatggc aggacgcttt
     4381 attttctgga tatttgcaaa aaaaaaacaa gatgtctttc aggataggct ctggagttga
     4441 ttttcaccaa ttgaccgaag gaagggagtt atggattggg ggtgtaaaaa ttccgcatca
     4501 taagggtgcc cttggccata gtgatgctga tgtattgttg catgctattt gtgatgcact
     4561 gcttggggca ttaagccttg gcgatattgg cttacatttt cctaataatg atcctgctta
     4621 caaaaatatt gacagtaagt tattgttgaa gaagaccatt gaaatggtca gggataaagg
     4681 gtaccgcatt gtaaatattg attcaacttt atgcctgcaa gagccaaaaa ttaaacccta
     4741 tgtatctgcc atgcagaaag taattgcaga tatttccggt attcagactt cagacgtatc
     4801 tgtaaaagcc acaacgacag aaaatatggg ttttgttggc cgtgaagaag gcctgatggc
     4861 ctatgctact gtactgctgg aaagaaaagc ctgacagaat cgaaattttt tccgtctgcc
     4921 gataactatc tttgccgcat gcccgcaata gagataaatg tcattaataa atcaacgaac
     4981 ccactccctg agtatgccac cgctggttct tccgggatgg acctcagggc aagtttgcag
     5041 gaacctgttt cgttcaggcc cctgcagagg ctgctcatac ctacgggcct ttttatagaa
     5101 ttaccggccg gctacgaggc ccaggtgagg cccagaagcg gattagctat taagcagggg
     5161 attacttgcc ttaatacacc tggcaccatt gatgctgact accggggaga aattaagata
     5221 atattgatca acctttctgg tgaggagcaa gtcataaacc atggtgaccg aattgcacaa
     5281 ttagtaatac aaaaagtgga aaaagcatgc tggaaagaaa cagacgaact ggccataact
     5341 gccagaaatg aagggggatt tggtcataca ggtcatcaat aacttaaatc ggcatgaatc
     5401 aattatcagt ttcacttatc gcaataactt gctttttata tgcctgccgg cctgcaagaa
     5461 atattcaaac tacccccccc gttgccgata caatttcaat agctgttact ccaaccgata
     5521 atccgaaaga agactctgcc agagttatta aagaaaatta tgatagaata ctagccggcc
     5581 agattcaatt cacaactttt aatgctaaga tcgatgtaga atacagggat gccgatggca
     5641 aagacatcaa tgccaatgct catttaagaa tgtataaaga cagcgtaata tggctttccc
     5701 ttactggtcc gttaggcatt gagggtatca gggcatatat aacaaaggat tctgtaaaac
     5761 tcctgaacaa acaggataaa atttatacgg cccgtagcgt ttcttattta caggaagtga
     5821 ctgaattgcc ccttgatctt tcctcactgc aaaacttgct ggccggtaat cccatttttt
     5881 ttgactcaac tattactgga tacagtcttt ctcccggaag catttcttta ttcagcgccg
     5941 gggagttctt taataacctt ttaaccatcg gtgagtctga taaatatctt caaagctgta
     6001 aactcaatga cctggactca acaagagcca gaacttgtta tctcacttat tcggcttacg
     6061 aaaataaaag gggatataat ttttcaacac ggcgctcaat tacggttaca gacaagaaaa
     6121 aactggatat tgaactggat ttcaggcaat atgactttaa tgaaacgtta agtttcccct
     6181 tttctattcc gaaaaattac aaacgtaatt aggtaataaa atttaaattg catctcatcg
     6241 ttattcagga attattccgc accctgtttt tataaaaccc atatcccttt agcatgagaa
     6301 aaataagctt actgatattc ttcattcttt ttgccggtat aacggtattt gcacaggata
     6361 aaactcaact ggaaaaagaa aggcagcaaa ttcagaagga gttacaagag atacagaccc
     6421 tttacaataa ggtaaaaggg caaaccaagc agacccttgg acagttgaat gtactgaaca
     6481 ggaagatcag tctttatgaa cagtatatca gcagtattaa taaagaaatc aagtctatag
     6541 atgatgatat ttatctgagt aacctggaaa tatatcgact gcaaaagcaa ctggatactt
     6601 tgaaaacaca gtatgcaaaa acaatagtct atgcttataa aaacaggagt agctatgatt
     6661 atctaaattt tatcttttcg gccaatagtt ttaatgatgc tgtaaaacga atttcttacc
     6721 tgaaaagcta caaagattac agggaaagac aggtaagtac aatccttgaa acccagcaat
     6781 tgattgcggc cagaaagaag caacaactgt caagaaa
//
LOCUS       DAORLS010000161         6806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6032_length_6806_cov_1.986372, whole genome shotgun sequence.
ACCESSION   DAORLS010000161 DAORLS010000000
VERSION     DAORLS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_6806_cov_1.986372"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK977_12125"
     CDS             complement(<1..218)
                     /locus_tag="PK977_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18911.1"
                     /translation="MKPLINHILSKLIALIAVCFFVNNLQAQVPTANFSGTPLSGCSP
                     LIVHFQDLSTGNPTAWNWNFGNGNTSVL"
     gene            complement(408..827)
                     /locus_tag="PK977_12130"
     CDS             complement(408..827)
                     /locus_tag="PK977_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18912.1"
                     /translation="MKTIQYLLVICLLLAGMTSCKKTIEQIQEDLVIKAMTDGQWKIT
                     NFVYNGNNITSDFDTYRFKYYSNKTVDAINNGTVEKTGNWDGNSSNMTTWANFTGAVH
                     PLVLINGNWQITRNSWTYVEASQTVGSDTKIMRLDKQ"
     assembly_gap    948..1121
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1122..>1412
                     /locus_tag="PK977_12135"
     CDS             <1122..>1412
                     /locus_tag="PK977_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18913.1"
                     /translation="NYLAGETAWFKAYLQSAFLPDTISTVLYVELLNRSSSVLTRKIL
                     PVLLGVTNGQIEIPDTLITGNYLVSAYTPTMLNNGSDFIFRKSVFIYGKKNE"
     assembly_gap    1413..1422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1423..>2257
                     /locus_tag="PK977_12140"
     CDS             <1423..>2257
                     /locus_tag="PK977_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18914.1"
                     /translation="GMTPRSVKLHFFPEGGNLVQGLTHTTAFKATDESGRPVGVNGEI
                     RDGKNNKITELNTFHDGMGLFDLSPVAGEKYHAVINGIADNYYLPDVAEKGISLTVIP
                     HPQGSFFEIKQQKGNTDFSAAYILGQMQHQLVFKKEFTAGREEIQGVIDTKKLNSGIL
                     QLTVFNKDHVPLAERLVFVDNKEYIQQAELIADSLSFQPKGRNRFSIKMRDTVQGSFS
                     VSVTDAGFLAYTKREENIFSQLLFTSDLKGYIHNPAYYFSAESDSVKTAMDLVMMTHG
                     WR"
     assembly_gap    2258..2393
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2394..3361
                     /locus_tag="PK977_12145"
     CDS             <2394..3361
                     /locus_tag="PK977_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18915.1"
                     /translation="ADSSKSAQIVLTDKKGDFRLDSLIVYGKARIFFSDTRGKKSLYI
                     DVELAGDTLSRNYDVVVDTSMIMNYNELTAGSLHHMSVDYAAILNEKGKMLEGVTISV
                     KKKTPLQELEEKYAKGPFEGGDAKTFDLVNTDENLIYNDLFDYLNAKGLMPGRRNQPM
                     LSSLGSFAVDYYLDEIQVDADVLQTIPFTHIAMIKVYNTFAGGWGNSPGGAVAVYMKK
                     GEDLLNAMSSHGRVITYNGFSVIKEFFAPDYKTNPADKNKTDNRITLDWRPSIFINNV
                     NPKIPLTFYNSDRTKDFKIIVEGMTTSGKMVCIEKTFSKADYIKD"
     gene            3403..5817
                     /locus_tag="PK977_12150"
     CDS             3403..5817
                     /locus_tag="PK977_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18916.1"
                     /translation="MKFFLISVLLLVVYSVKCQQPEYFINKWADRSPLEKVYLQTDRD
                     NYSGGETIWFKAYLSSDYFPDTISTTLLTDLVNINGQLISRKAWPVVYGAAKGQFEIP
                     DSISSGSYILRAYSPTMLNHDHDFLFSRTIGIVGKSPVIKETGDKKVRMELFPESGNF
                     IAGFPNTVSFKITDENGLPLNLSGHIMNSKGDTITTFNTYHDGMGFFDVIPLANVRYY
                     AVINEDPGQNKYYLPPANTEGIVFRLLPAEKGNDFEIYQRWNDTTFRAAYIVGLMQHR
                     TVLRKKLEKSRNDITGFIDTRQLRSGVLHLTVFSENGLPLAERLVFVNNKEYLQTATL
                     VTDTINLIKRGKNRFTLSFPDSVAGNFSLAVIDADYETGEKQAPNIITSLLLTSDIRG
                     NIHNPAWYFTAAPDSAKNGLDLVMMVNGWRRFKWTEVEKLATATPVYRDPGFITVSGQ
                     IKIRDTKKALSDKELLLMMSSLQDSVSSSMQMINTDNTGSFRLDSMIFSGTARFLFSD
                     ISEKKKKWLDVKADVDSVRTAYIISPADRNKFFSGSSLLSDKIKNKLAFDYENILKAE
                     GQLLTGITVKVRKKSPLQELEEKYVTGMFSGISAKTIDLVNTNEKIYQNNIFDYIQGR
                     VAGVSVRKDGLNYTLYYRQRFSLTGGAIPMTLYLDEMQVDGRLLATIPASQVAMIKVY
                     SSFFGAEGNGVGGVLAVYTKKGAEFSGYSAVSSDAIIFKGYAVVKEFYSPDYSVKAAD
                     TKEDKRITLLWQPDIIAEGKDVQIPVVFFNNDRAKSIKIVAEGMTADGKLLYLEKIIP
                     GIQKVF"
     gene            complement(5885..6508)
                     /locus_tag="PK977_12155"
     CDS             complement(5885..6508)
                     /locus_tag="PK977_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF18917.1"
                     /translation="MIKRKMGRYLLTLLVVTGLAGTIEDNTISKSERKYSITLMKETY
                     KEAVKATKGLSEAQLNFKAAPDKWSVKDCIYHIAAVEKMLWGMFEGAMKAPANPEKRS
                     EIKLSDEQVVNMVEDRSRKVQAPEPAQPRNTGYSSLDDALADFKKTRGEHIKYMKTST
                     EDMRNHVVQTPVGWLDCYQVYLFIAAHSNRHTQQINEVKKDPSFPSK"
     gene            complement(6623..>6806)
                     /locus_tag="PK977_12160"
     CDS             complement(6623..>6806)
                     /locus_tag="PK977_12160"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRF18918.1"
                     /translation="GFGKRGESYTNTDIIFTVVTRLEISRLTTEVEKIDPHAFVVMQS
                     IRDTKGGMVKKKPLAH"
BASE COUNT         1949 a         1203 c         1397 g         1937 t
ORIGIN      
        1 tgcagtacag aagtgttgcc gttcccaaaa ttccagttcc atgctgtagg attgccggtt
       61 gacagatcct ggaaatgaac tattaacgga gaacaacctg aaagtggtgt accggaaaaa
      121 ttggccgtgg gaacctgtgc ctgcaaattg ttcacaaaaa agcaaactgc aatcagtgca
      181 attagtttgg agaggatatg gtttatcaaa ggtttcaaca ggtggctgtt ttggttttca
      241 acttcgaagg tagcaagggg tgcaaaaact gttcctgatt actgcttgtt ctcagaatta
      301 ttttttggaa aataaaacat gcacttctgt gaccttcaga cctctttttt gtttaaaagg
      361 cagggaacgg atcgattatt attattaacg aaaaatctgt gtgattatta ttgcttgtca
      421 agccgcataa tcttggtatc gctacccacg gtttgactgg cttctacgta ggtccaactg
      481 ttacgggtta tctgccagtt tccgttgata agtaccagag gatgaacagc tccggtaaaa
      541 ttagcccagg tagtcatatt gctggaattg ccatcccaat tcccggtttt ttcaactgta
      601 ccgttattaa tggcatcaac tgttttatta ctgtaatact tgaacctgta ggtgtcaaaa
      661 tctgaagtaa tattattacc gttatagaca aagttggtga tcttccattg accatcggtc
      721 atggctttta tcaccaggtc ttcctggatc tgctctatcg tttttttaca actggtcatt
      781 cctgcaagca gcaggcaaat aaccaggaga tattgtattg ttttcacaag cacaaaataa
      841 gtaatctgta tcgcttatcc aacagtagaa gaacgcagtt tctttactta aagatttgtt
      901 taaaaagcag ttaggccttt tggcattaat tagtttggct ttaccttnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacaactacc tggcgggtga
     1141 aactgcctgg tttaaagctt acctgcaatc tgctttttta cctgatacca tatccactgt
     1201 tttgtatgtg gaattactaa acaggtcatc atctgtctta acaagaaaga ttttacctgt
     1261 tcttttaggt gttaccaatg ggcagataga aattcctgat accctgatta ccgggaacta
     1321 tttggtcagt gcttatacac ctacaatgct caataacgga tctgacttta tttttcgtaa
     1381 gtcagtattt atttatggta aaaaaaatga gcnnnnnnnn nntggtatga caccccgatc
     1441 agtcaaactt catttttttc ctgagggagg aaatttagtt cagggactta cacatacaac
     1501 cgcatttaaa gctacagatg aatcaggaag gcctgttgga gttaacggag agatcaggga
     1561 tggtaaaaat aacaagataa cagagctgaa tacttttcac gatggtatgg ggcttttcga
     1621 tctttcacct gttgccggtg aaaaatatca tgcggtaata aatggcattg ctgataatta
     1681 ttacttgcca gatgtggctg aaaaaggaat cagtttgact gttatacctc atccgcaggg
     1741 aagctttttt gaaataaaac agcaaaaagg gaatactgat tttagtgcag cttacatttt
     1801 ggggcaaatg cagcaccagt tggttttcaa aaaagaattt acagcaggaa gggaggaaat
     1861 acagggagta attgatacca aaaagctaaa ctccgggata cttcaattaa cggttttcaa
     1921 taaagatcat gttcctcttg ctgaaagact ggtgtttgta gacaataaag aatatataca
     1981 gcaggctgaa cttatagctg attcgttaag ttttcagccc aaaggaagaa atcgcttttc
     2041 cataaagatg agggatactg tgcagggtag tttctctgtt tcagtaaccg atgcaggatt
     2101 tttggcatat actaaaagag aagaaaacat cttttctcaa ttattattta cctccgatct
     2161 gaaagggtat atccataacc cggcatacta tttttctgcg gaatctgatt ctgttaaaac
     2221 tgctatggat ttagtgatga tgactcatgg atggaggnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgcgga
     2401 ttcatcaaaa agtgcacaga ttgttttgac ggataaaaaa ggagacttcc ggcttgattc
     2461 attgattgtt tacggtaaag ccagaatctt cttcagcgat acaagaggaa agaagagttt
     2521 gtatattgat gtggaactgg ctggtgatac attgagtcgc aattatgatg ttgtggtgga
     2581 taccagcatg atcatgaatt acaatgaact gactgcaggt agtctgcacc acatgtcagt
     2641 tgattacgca gcgatattaa atgaaaaagg gaaaatgctg gagggggtga ccatcagcgt
     2701 aaagaaaaaa acaccgctgc aggaactgga agagaaatat gcaaaagggc cttttgaagg
     2761 gggagatgct aaaacctttg atcttgttaa tacggacgaa aatcttattt ataatgacct
     2821 ttttgattac ctcaatgcaa aaggtttgat gcccggtcgc agaaatcagc cgatgcttag
     2881 ttcgctgggt tcatttgcag ttgactatta cctggacgaa atacaggtgg atgcagatgt
     2941 tcttcaaaca ataccattta cccatattgc tatgatcaaa gtatacaaca catttgctgg
     3001 cggatggggc aattctcccg gaggggcagt ggctgtgtat atgaaaaagg gggaggattt
     3061 gctcaatgcc atgtcctctc atggcagggt aataacatac aacggctttt ctgttattaa
     3121 ggaatttttc gcccctgact ataaaactaa tcctgcggat aagaataaga cagacaaccg
     3181 tatcaccctc gactggagac cttctatatt tatcaacaac gtaaatccta aaattccact
     3241 tactttttat aacagcgacc gcacaaagga tttcaaaatt attgtagaag ggatgaccac
     3301 ttccggaaaa atggtttgta ttgagaaaac cttctcaaaa gctgattata tcaaagatta
     3361 aaaactaaaa gcataattat taattagcct ccttgtctta ttatgaaatt ctttttaatc
     3421 agcgtattgt tactggtagt atattccgtt aagtgccagc aaccagaata ttttattaat
     3481 aaatgggctg acagatcgcc tcttgagaaa gtatacctgc agactgacag ggataattat
     3541 tccggtggcg aaacgatttg gttcaaagcc taccttagtt cagattattt tccggatact
     3601 atcagtacca cattgcttac cgatctggta aacatcaacg ggcagttgat ttcccggaaa
     3661 gcatggccag ttgtatatgg tgcggcaaaa ggccagtttg aaataccgga ttccatttct
     3721 tccggttcat acattttaag ggcttattcg cccaccatgc ttaaccatga tcatgatttt
     3781 cttttcagca gaaccatagg aattgtgggt aaaagcccag tgataaagga aacaggtgat
     3841 aaaaaagtca gaatggaatt gtttcctgaa agcggcaact ttattgcagg cttcccgaat
     3901 acagtatctt ttaaaattac tgacgagaat ggattgccgc tgaatctgtc gggtcatata
     3961 atgaatagca aaggggatac cattactact tttaatacgt atcatgacgg aatgggattt
     4021 tttgatgtaa ttccattggc taatgttcgg tactatgcgg taatcaatga ggatccgggg
     4081 caaaataaat attatctgcc accggctaat accgagggaa tcgttttcag gttattgccg
     4141 gcagaaaagg gtaatgactt tgaaatatat cagcgatgga acgatacaac tttcagggca
     4201 gcatatatcg ttggactgat gcaacaccgt acggttctgc gaaagaaact ggaaaagtcg
     4261 agaaatgata taacgggctt tatcgacact cgtcagcttc gctcgggggt attgcatctt
     4321 accgttttca gcgaaaacgg gttaccgctt gccgaacgtc ttgtatttgt gaataacaaa
     4381 gaatatttgc aaaccgcaac actagttaca gataccataa atcttattaa gcggggaaaa
     4441 aacaggttca ccctgtcttt tcctgattct gtggctggta atttttccct tgcagttatt
     4501 gatgcagatt acgagacagg cgaaaagcag gcaccaaata ttattacttc cctcttgctt
     4561 acttctgata taagaggtaa catacataac cctgcctggt attttactgc agcaccagat
     4621 tctgcaaaaa atggacttga ccttgtaatg atggtgaatg gctggagaag gtttaagtgg
     4681 acagaagtcg aaaaattagc aacagcaacc cctgtttaca gggatccggg atttataaca
     4741 gttagcgggc aaatcaaaat acgggatacc aaaaaggccc tctcagataa agaacttttg
     4801 cttatgatgt ccagcttaca ggattcggta agcagcagta tgcaaatgat caatacagac
     4861 aatacgggaa gttttcggct ggactcaatg attttttccg gcaccgcaag attccttttc
     4921 agtgatataa gcgagaagaa aaaaaaatgg cttgatgtta aagccgacgt tgactctgtc
     4981 cgtacagcat atataataag cccagcagac aggaataagt ttttttctgg cagtagcctt
     5041 ctttctgaca agataaaaaa caaacttgct tttgattatg agaatatttt aaaagcagaa
     5101 ggacaacttc ttaccggaat tactgtaaaa gtccgaaaaa aatcaccatt acaggaactg
     5161 gaagaaaaat atgtaaccgg tatgttctca ggaatttctg caaagacaat tgatctggta
     5221 aatacaaatg aaaagattta ccagaataat atcttcgatt atatacaagg gcgggtggca
     5281 ggcgtttctg taagaaaaga cgggctaaat tatacattgt attaccgtca gcgtttctcg
     5341 ctgacaggag gtgccatacc tatgacttta tacctggacg aaatgcaggt agacggcagg
     5401 cttctggcaa ctatacctgc cagccaggta gcaatgataa aagtatacag ttcatttttt
     5461 ggtgccgaag gaaatggggt gggtggtgta ttggctgttt acacaaaaaa aggggcagaa
     5521 ttttctggtt actcagctgt atcatctgat gcaataatat tcaagggata tgctgttgtg
     5581 aaagagtttt attcaccgga ttattctgta aaagcagcag acacaaaaga ggataaaaga
     5641 ataacgctgc tatggcagcc tgacattatt gcggaaggca aggatgtaca aatcccggtt
     5701 gtttttttta ataatgacag agcaaaatcc attaaaatcg ttgctgaagg aatgactgcg
     5761 gatggcaaat tgctttacct ggaaaaaatt attccgggaa ttcagaaagt attttaaaac
     5821 taagaccccc tgttcttacg ggaacaaggg gccaggtttt tcaactgttg aaaaagagtt
     5881 gaagtcactt agaagggaag gaggggtcct ttttcacttc atttatttgt tgtgtatgac
     5941 ggttactgtg tgcagcaatg aacaggtata cctggtagca atcaagccat cccacaggag
     6001 tttgcactac atgattacgc atatcctctg tggaggtctt catgtatttg atatgctccc
     6061 ctcttgtttt cttaaaatct gccaatgcgt cgtcaagtga actgtaaccg gtattcctgg
     6121 gttgtgctgg ttcaggtgcc tgtacttttc ggcttctgtc ctctaccata ttcactacct
     6181 gctcatctga taattttatt tcagaccgtt tctcagggtt ggctggggcc ttcatagctc
     6241 cttcaaacat tccccacagc attttttcta cagcagcaat atggtaaatg cagtctttaa
     6301 ctgaccattt atccggcgct gctttaaagt tgagttgtgc ttccgacaga ccttttgtgg
     6361 ctttcacagc ttctttatat gtttctttca tcagggtgat ggaatatttc ctttctgatt
     6421 tggaaattgt attatcttct atagtgcctg ccaggccggt aacaaccagt aaggtgagaa
     6481 gatagcggcc cattttacgt ttgatcataa caactgtttt gtcggttaat gggatatgga
     6541 agctatccag agggcgggaa aggaaagtta caaagtattt ccattcagac aatatcaatt
     6601 ttgtaacatg tatgctgaat tatcaatgtg ccaggggctt tttctttacc ataccacctt
     6661 tagtgtccct gatactttgc atcaccacaa aggcatgcgg gtcaattttt tccacttcgg
     6721 ttgtcagccg gcttatttcc agtcgtgtta ccacagtaaa aatgatgtct gtgttggtat
     6781 agctttcacc tcttttgcca aagcct
//
LOCUS       DAORLS010000162         6795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6043_length_6795_cov_2.020623, whole genome shotgun sequence.
ACCESSION   DAORLS010000162 DAORLS010000000
VERSION     DAORLS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_6795_cov_2.020623"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PK977_12165"
     CDS             <1..529
                     /locus_tag="PK977_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF18919.1"
                     /translation="EDMYVILLDNGRTNILANEKQRESLYCIRCGACLNACPVYKNIG
                     GHTYAATYSGPIGSVITPHLKDMGEWKHLSYASSLCGNCTEVCAVKINLHELLLENRH
                     ESVESGYGSFTEKMTWKMWKQAMLRRKWMNMANGGMKNRVVNSMMKSWTAHRGKLEFP
                     QKSFNQLWREKNSHK"
     gene            535..1263
                     /locus_tag="PK977_12170"
     CDS             535..1263
                     /locus_tag="PK977_12170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF18920.1"
                     /translation="MPGISSPAFFMSAQIKYYNQFGNAYRQSILSCPEPELWTTDYAE
                     KGRVYNEMKSRIEQQQQLIDNYFSVQWPVLDYGCGFGRQAFILAKKGYEVVGCDSSEV
                     FISIAKELFEKSQFKGEFLVGGIDHLQGRHFSQAVLFDVIEHIPPGKKKELVTQLASV
                     LIHDGVLLMSLPHVKKRLSSQLNNSIRRRITGLFAYFRNREEHPYYIPQQKDILQLFG
                     KQFSLDSFTAADETDYYVFRRKKE"
     gene            1341..>1817
                     /locus_tag="PK977_12175"
     CDS             1341..>1817
                     /locus_tag="PK977_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18921.1"
                     /translation="MSVTDNVIQYFDHPGPRINYSNEPLSQQEFWIKPQGLLHEAGIR
                     QFSIECFEYNGNIAFFRTEGDFPFDILAASFFLLSRYEEYLPHTKDTYGRYAHENSLA
                     YKVGFLNKPLINYWLIEFKKSLQLKFTSQTPDSFWDHHSPFTFLPTYDIDEAYSYKY"
     assembly_gap    1818..2009
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2010..2583
                     /locus_tag="PK977_12180"
     CDS             <2010..2583
                     /locus_tag="PK977_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18922.1"
                     /translation="TKKYDRTILPSEAALQTLIRDHAEKYDLGIHPSWQSGDDQSLVK
                     KEIQTLEKISKQKITASRQHFIRFTLPQTYWQLIGAGIKEDFSMGYGSINGFRASVAS
                     PFYWYDLEKEEQTYLLLYPFCYMEANSFFEQKQTPQQALEEMLQYYQTVKSVNGTLIT
                     IWHNTFLGTDELYKGWRDAYYQFFNHVMKG"
     gene            complement(2584..3594)
                     /locus_tag="PK977_12185"
     CDS             complement(2584..3594)
                     /locus_tag="PK977_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRF18923.1"
                     /translation="MNIFDFHKSIIQTSADYFKKLAAFNPLTIRKKGTRAKALFALAL
                     VCFFWGTTWIASKEGVRHMPALQLAGIRQLIAGLLYVAFFLYKKAPLPKGKEWTPILI
                     LSFLNFILSNGLSTWGVKYISAGLGSIMGAIFPLWLVVIGLFVSKEQLPRNAIIGLLL
                     GFGGVCVIFYEHLLDFGNPEFRFGIILSLIATWTWAFATLYTKQQAVHFNPYFSLGLQ
                     MLIAGTSLTVFTNVTGNAVSMTVIPWQSWSAIAYLIIFGSLIAFIAYLYALQNLPTEQ
                     ASIYAYINPIVAVLCGWLVFHEKVTIFITVGGLVTLLGVYLVNKAFKAVPPPEQPETE
                     GM"
     gene            complement(3678..5183)
                     /gene="purH"
                     /locus_tag="PK977_12190"
     CDS             complement(3678..5183)
                     /gene="purH"
                     /locus_tag="PK977_12190"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRF18924.1"
                     /translation="MKKKIQSALISVFYKDGLETLVQTLATQGVQIYSTGGTQAFIEK
                     LGVAVTPVESLTNYPSILGGRVKTLHPLVFGGILGKRDDTQHVQDMQQYKIPEIDLVI
                     VDLYPFEETVKSTTDEIAIIEKIDIGGPSMLRAAAKNHKDVVVIADKKDYAVLNQLLN
                     DQQGATTYDQRRAFAIKAFDVCTAYDTAISNYFHGTAIESPFNKPEKSLRYGENPHQK
                     AVFYGDLELLFTQLNGKELSFNNLVDVDAALQLIREFNDTTFAIIKHTNVCGVAQRPS
                     VKESWDAALAGDPESAFGGVLVCNGTIDKVTADAINEIFFEVLIAPSFDEDALATLKS
                     KKNRILLQLNEQPQGKLQYKCVLNGMLVQDTDEGNFIDWTETVKRKVTETEKQDLLFA
                     NIICKHLKSNAIALVKDKQLVGKGCGQTSRIDSLRQALDKAKQFEFDLNGAVMASDAF
                     FPFDDCVRLGHQAGITAFIQPGGSIRDNDSIEYCKEHNLALVMTGMRHFKH"
     gene            complement(5257..>6795)
                     /locus_tag="PK977_12195"
     CDS             complement(5257..>6795)
                     /locus_tag="PK977_12195"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRF18925.1"
                     /translation="LFQGITHQVYLKPEEFETLSGKNEKWFRKFIYSSREKVLNILRQ
                     NITEEKELGLAEALLIGYKNDLEQSLVQSYTNTGVVHIIAISGLHLGLIYWLLSLLLK
                     PLQKAKRMKWLRPVLTITGLWLFSLLAGAQPSILRSALMFTCIVLGESLSRRTSIYNT
                     MAVSAFLLLCINPFWLWDVGFQLSYAAVLSIIIFMRPVYNWFYIPNKLLDFIWKLNAV
                     TIAAQILTVPIGIYHFHQFPNLFLLTNFVAVPLSSLILLGEILLCCISFIPPVAVFIG
                     KLLSFLIRTMNRYIEEIETIRFSLWDGLHISITQAVLLLIFTTGISYWLMEKQKKGLI
                     TGLVALLSFFSLRSYSFAESERQQKIVVYNVPQKRAIDFINGRNYYFAGDSDLLADDF
                     ARNFHLKPSRIRFRIKEYRQLDNLTIYDNYVRFLNKNVLLLDQSVQFKSVEKKPVVDL
                     LVISKNPRLYIGKLSKTLDIKQVVFDGSVPSWKSRYWKKDCDSLQIPWHDVAIQGAFV
                     MKLN"
BASE COUNT         1989 a         1353 c         1382 g         1879 t
ORIGIN      
        1 ggaagatatg tatgtgatat tgctggataa tggcagaaca aatattcttg ccaatgaaaa
       61 acaacgggaa agtttgtatt gtattcgttg cggcgcctgc ctgaatgcct gtcccgttta
      121 taagaatatc ggcggtcata cgtatgctgc cacttacagc ggacctatag gttcagtcat
      181 tacaccgcat ttaaaagata tgggtgaatg gaaacacctg agttatgctt cttcgctgtg
      241 tggtaattgc actgaagtgt gtgccgtaaa aattaacctg catgaactgt tgctggaaaa
      301 tcgccatgaa tcagtggaga gtggctatgg ctctttcaca gaaaaaatga cctggaagat
      361 gtggaagcag gctatgctgc gccgcaaatg gatgaatatg gccaatggtg gtatgaagaa
      421 cagggtagta aacagtatga tgaaaagctg gacagctcac cgtggcaaac tggagtttcc
      481 ccaaaaatca tttaaccagc tatggagaga gaaaaatagt cacaaataaa gcatatgccg
      541 ggtatttcat caccggcttt ttttatgtct gcccaaataa aatactataa ccagtttggc
      601 aatgcttacc ggcagagtat tctttcctgc ccggaacctg agctatggac gactgattat
      661 gctgaaaaag gaagggtcta caacgaaatg aaatcaagga ttgaacagca acaacaattg
      721 atagacaact atttttctgt tcagtggccg gtgctggatt acggctgtgg ctttggcagg
      781 caggcattca ttttggcaaa aaaaggatat gaagttgtgg gctgtgacag tagcgaagtg
      841 tttatcagta ttgctaaaga gttgtttgaa aaatctcaat ttaaaggaga gtttttggta
      901 ggaggtattg atcacttgca aggcaggcat ttcagccagg ctgtgctttt tgatgttatt
      961 gagcacattc cccctggtaa aaaaaaagaa ctggtaactc agctggcctc tgtccttatt
     1021 catgatggtg ttttgctcat gtcactaccc catgtgaaaa aacgactcag ttcccagcta
     1081 aacaattcca tcagaagacg cataaccggg ttattcgctt atttccgcaa ccgggaagag
     1141 catccgtatt atatcccgca gcaaaaagat atacttcagc ttttcggcaa acagtttagt
     1201 ttggatagtt ttacggcagc ggatgaaaca gattattatg ttttccgcag gaaaaaggaa
     1261 tgatcattta cagccataac attacccatc gattaaagta catggcctat tttttgggtc
     1321 aaaatttgac aggtgagcca ttgagtgtca ctgacaatgt cattcaatac tttgatcatc
     1381 ccggaccaag aattaattat agtaatgaac ccctatctca acaggagttt tggataaagc
     1441 cacagggcct gttgcatgaa gcaggcatcc ggcagttctc catcgaatgt tttgaatata
     1501 atggcaatat tgcttttttc cgcacagaag gtgatttccc ttttgatatt ttagcagctt
     1561 cctttttcct gctcagccga tacgaagaat atttaccgca tacaaaagat acgtatggac
     1621 ggtatgcaca tgaaaactca ttggcatata aagtaggatt tttgaataag ccgttaatca
     1681 actattggct tattgaattc aagaaaagtt tgcaactgaa gttcacttca caaactcccg
     1741 atagtttttg ggatcaccac tcacctttca cctttctccc tacttacgat attgatgaag
     1801 catattccta taagtacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna acaaaaaaat atgaccggac cattcttcct
     2041 tcagaagcag ctttacaaac attgatccgg gatcatgcag aaaaatatga tctgggtatt
     2101 cacccttcct ggcagagtgg tgatgatcaa tcattggtta aaaaagaaat acagacactg
     2161 gaaaaaatca gcaaacaaaa aatcactgct tcccgccagc attttatccg tttcacacta
     2221 cctcaaacct actggcagct gattggggct ggtatcaaag aagatttttc catgggctat
     2281 ggaagtatca acggctttcg ggcatcggtg gcttcaccat tttattggta tgacctggaa
     2341 aaagaagaac aaacatatct gttgctctat cctttttgct acatggaggc taattctttc
     2401 tttgaacaaa aacaaacccc gcagcaggca ctggaagaga tgttacagta ctatcaaacc
     2461 gtcaaatcgg taaatggcac attgatcact atatggcaca atactttttt aggaacggat
     2521 gaattgtata aaggctggag agatgcctat taccagtttt ttaaccatgt aatgaagggt
     2581 tgatcacatg ccttctgttt ctggttgctc aggcggtggt actgctttaa aggctttgtt
     2641 taccaggtat acacccagca atgttaccaa tcctccaact gtgatgaata tggttacttt
     2701 ttcatgaaat actaaccacc cgcaaagcac cgctacaatg ggattgatat aagcataaat
     2761 agaagcttgt tccgttggca aattttgcaa agcatataaa taagcaatga aggcaatcaa
     2821 cgaaccaaaa atgataaggt aggcaatagc agaccatgat tgccatggaa taaccgtcat
     2881 ggatacggca ttaccggtaa cattggtaaa tacagttaat gaagtgccgg ctatcagcat
     2941 ctgcaaaccc aaactgaaat agggattaaa atgtacagct tgttgcttag tgtatagcgt
     3001 agcaaaagcc caggtccagg tagcaatcag tgagaggatg atgccaaacc gaaattccgg
     3061 attgccaaaa tccaataagt gctcatagaa aataacacat acaccaccaa aacccagcaa
     3121 taaaccgatg attgcattgc ggggcagttg ctcttttgaa acaaataaac cgatcaccac
     3181 gagccagagc ggaaaaatgg cgcccataat ggagccaagg ccggcagaaa tatattttac
     3241 tccccatgta ctcagtccat tgctgaggat gaaattgagg aagctcagaa taagtatcgg
     3301 tgtccattct tttcctttcg gtaaaggagc ttttttgtaa agaaagaagg ctacatacag
     3361 caatccggcg attagctgac gtataccggc cagttgtaat gccggcatat gccgaacacc
     3421 ttcctttgaa gcgatccagg tagtgcccca aaaaaagcag actaatgcca gcgcaaataa
     3481 agctttggcc cttgttccct ttttccggat ggtaagggga ttaaaagcgg ctaacttttt
     3541 gaaataatca gcagaagttt gaataataga tttatggaag tcgaaaatgt tcatgactgc
     3601 tgacgctgct ttatgctcgg taatatattt agtttgcccg aatgaaaata aagaaaaccc
     3661 cttgaggggg tcagggctta atgtttaaaa tgcctcattc ctgtcatcac taaagccagg
     3721 ttatgttctt tgcaatactc aattgaatca ttatctctga ttgatccgcc gggttgaata
     3781 aaggcagtaa tgcctgcctg gtgtcccagt cgcacacaat catcaaacgg gaagaaagca
     3841 tcgctggcca ttacagcgcc attcaggtca aattcaaatt gctttgcttt atccaatgcc
     3901 tggcgcaagg aatcaatacg gctggtttgt ccgcaaccct tgcctaccaa ttgcttgtcc
     3961 ttcactaaag caatggcgtt tgatttaaga tgtttacaaa tgatgttggc aaacaagagg
     4021 tcttgttttt ctgtttctgt cacttttctt tttacagttt cggtccagtc aataaagtta
     4081 ccttcatcag tatcctgtac cagcataccg ttgagtacac atttgtattg cagttttcct
     4141 tgtggttgtt cgttcagctg cagcaatatg cggttcttct ttgacttcag agtggccagt
     4201 gcatcttcat caaatgaagg tgcaatcagc acttcgaaga atatctcgtt gatagcatcg
     4261 gcagttactt tatcaatggt gccattgcat accagcacac cgccgaaagc actttccggg
     4321 tcgccggcca gggctgcatc ccagctttct tttacagatg gacgttgcgc tacaccacac
     4381 acatttgtat gtttgattat cgcaaaggtt gtatcgttaa attctcttat cagttgtaag
     4441 gctgcatcca catccaccag gttattaaag gaaagttctt taccattcaa ttgagtgaac
     4501 agtagctcaa gatctccgta aaacactgcc ttttgatggg ggttttcgcc gtaacgtaaa
     4561 gacttctcag gtttattgaa aggcgattca attgctgttc catgaaaata attggaaata
     4621 gcagtatcat aggctgtaca cacatcaaat gcctttatgg caaaagctct gcgctgatca
     4681 taggtagtgg ctccctgctg gtcattcagc agctggttca gtacagcata atctttttta
     4741 tcagctatca caacaacatc tttatgattt tttgctgctg cccgtaacat tgaaggtccg
     4801 ccaatatcaa ttttttcaat aatagcaatc tcgtcagtcg tagattttac tgtttcttca
     4861 aaggggtaca ggtcaactat cacaagatca atttcgggta ttttatattg ctgcatatcc
     4921 tgcacatgct gtgtatcatc ccttttgcca agtatgcccc caaatacaag agggtgaagt
     4981 gtttttaccc tgccgccaag aatagatggg tagttggtca gcgattctac aggtgttaca
     5041 gctacaccca atttttctat aaaagcctgg gtgcctcctg ttgaatagat ctgcacacct
     5101 tgtgtagcca atgtttgcac cagtgtttcc agtccgtcct tgtaaaaaac cgagattagt
     5161 gccgattgta tcttcttttt catagctgtt gggggttgaa agcagcaaat cgttgagcag
     5221 gccatgcatt gccggcatgg caggcgcaaa actaacctaa ttcagcttca tcacaaaagc
     5281 accttgtata gctacatcat gccagggaat ttgcagcgaa tcgcaatcct ttttccaata
     5341 acggctcttc caggagggca cagagccatc aaataccact tgttttatat ccaatgtttt
     5401 ggaaagtttg ccgatatata accttgggtt tttagaaatg accagaaggt caacaaccgg
     5461 tttcttttct acggacttaa attgaacaga ttgatctaat aacaacacat tcttattcag
     5521 aaaacggaca taattatcat aaatggtcag gttgtcaagt tgccggtatt ccttgatcct
     5581 gaaacgaatt ctagatggtt tcaggtgaaa attccgggca aaatcatctg ccagcaagtc
     5641 agagtcgccg gcgaagtaat agtttcttcc attaataaaa tcaatcgccc ttttttgagg
     5701 tacattgtag acaacaattt tttgttgcct ctcactttct gcgaatgaat aggagcgcag
     5761 ggaaaaaaag cttagtaatg ccactaaacc tgtgatcaat ccttttttct gtttttccat
     5821 tagccagtaa ctaataccag tggtaaaaat taataaaagg acagcctggg tgatgctaat
     5881 atgtaaccca tcccacaatg aaaatcggat ggtttcaatc tcttcaatat agcggttcat
     5941 tgtcctaata aggaatgata acagcttacc aataaagacg gctacaggcg ggataaatga
     6001 aatacagcat agcaatattt cccccaatag tatcaaactg gaaagcggta ctgcaacgaa
     6061 attggtcagc aagaaaagat tggggaactg gtgaaaatga taaatgccaa tgggtaccgt
     6121 cagtatttga gcagcaatcg ttacggcatt tagtttccag atgaaatcga ggagtttgtt
     6181 ggggatataa aaccagttat agaccggccg catgaagatg atgatactaa gtaccgctgc
     6241 ataagacaat tgaaagccta catcccaaag ccagaatgga ttgatgcaaa gcagcagaaa
     6301 ggcagatact gccatcgtat tataaatgga agttcttctt gataatgatt cacccagcac
     6361 aatgcaggtg aacatgagtg ctgaacgaag aatggatggc tgtgcacctg ccagcaggct
     6421 gaatagccat aaaccggtta tggtcaaaac tggtctgagc catttcattc ttttcgcttt
     6481 ttgcaacggt tttagtaaca ggctgagcag ccaataaata agaccaaggt gcaaacccga
     6541 tatggcaatg atatgtacaa ccccggtatt ggtataagat tgtaccaatg attgttcaag
     6601 atcattttta taaccaatca ataaagcctc tgcaaggcca agttctttct cttctgtaat
     6661 attttgtctc agaatattca gcactttttc ccgggatgaa taaataaatt ttctgaacca
     6721 tttttcattt ttgccggaca gtgtttcaaa ttcttccggt tttaggtata cctggtgagt
     6781 aataccctgg aaaag
//
LOCUS       DAORLS010000163         6771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6080_length_6771_cov_1.641900, whole genome shotgun sequence.
ACCESSION   DAORLS010000163 DAORLS010000000
VERSION     DAORLS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6080_length_6771_cov_1.641900"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK977_12200"
     CDS             complement(<1..538)
                     /locus_tag="PK977_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18926.1"
                     /translation="MKKNILFLLFFTCARLSFGQIRVFPEIINEIPRAYSDGKFVDTL
                     ENIYNSLISEQSDSIFLLYTYIEGYNFATIFWKKNGLTNSSAVYQINPKKGSEIKVEI
                     LNNRVFNSINIRSVYAIMQDSFVRMIDTLVYLTHDDPAYTYFFFDKRLSLYAGFSRMI
                     FGRIGLEFWNAHIKEEIRI"
     gene            complement(577..>760)
                     /locus_tag="PK977_12205"
     CDS             complement(577..>760)
                     /locus_tag="PK977_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18927.1"
                     /translation="VVFIPHGKHTSIRATNAKLTLNKKVNQKGQVFLGNISFQVTENL
                     KNKEYLFKGAFLSTLE"
     assembly_gap    761..916
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<917..1135)
                     /locus_tag="PK977_12210"
     CDS             complement(<917..1135)
                     /locus_tag="PK977_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18928.1"
                     /translation="MKYFTVILLICFIACDAQIPKEKQEFSIDKTLENKFSEGPLPGV
                     RGGAYKTYLDGVLVDSSNEDGIQYLYNAS"
     gene            1228..2043
                     /gene="prmA"
                     /locus_tag="PK977_12215"
     CDS             1228..2043
                     /gene="prmA"
                     /locus_tag="PK977_12215"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRF18929.1"
                     /translation="MPNHIQITFHELQPEQQEVLIAHLAEAGYEGFEQSDTELKAFIH
                     DSHYDKALLHELAFKYQVQYTEEHITAQNWNALWESNFQPVVVNDFVGIRADFHEPLQ
                     NVRHEIVITPKMSFGTGHHATTYMMIEQMGEIDFKGKTIFDFGTGTGVLAILAEKLGA
                     AYVLAIDNDEWSISNATENCQRNGCQHIQLQLADTAATGESYDIILANINKNVILDNL
                     VSLTSQLKKGGVLLLSGLLAEDENDILAAMQPYGLKPGQKSEKNNWIALRFNG"
     gene            2144..2815
                     /gene="plsY"
                     /locus_tag="PK977_12220"
     CDS             2144..2815
                     /gene="plsY"
                     /locus_tag="PK977_12220"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRF18930.1"
                     /translation="MNELLLIILAYLIGSVPTSVWVSKFFFDIDIRDYGSGNAGATNT
                     YRVLGPKWGTIVMVTDMLKGIIAVKLALLLPEYADNEVNLQNLQTGLGLAAVIGHIFP
                     IWADFRGGKGVATLFGLVLGISPWTALSCVGIFMLVLYLTRFVSLSSILASIAFPIFI
                     LVIFNVDNPAYRVFAIAVALMVLLTHQKNIGRLLRGSESKVPILKGRDRRRQRRRDAA
                     EHQDH"
     gene            2905..3723
                     /locus_tag="PK977_12225"
     CDS             2905..3723
                     /locus_tag="PK977_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF18931.1"
                     /translation="MIRIISSLFLLSAFLISCSKNPLSGKNQLTLLPESELQTMATGE
                     YQKFLSTNKVVAVSNNRDAAMVNRVGDRIVRAVETYYADKGMSDKLNGFKWETNLVED
                     KAVNAWCMPGGKIVVYTGILPITQNEAALAAVMGHEVSHALLQHGNQRMSQGIIQQLG
                     GVALQVALANKPQETQNLFLTAYGVGSTVGVMLPFSRKHELEADRYGLIWTAMAGYNP
                     QEAIGLWERMEKASNGQTPPEFLSTHPSEGRRIEQLKQYMPEALQYYKPVNGGK"
     gene            3900..4349
                     /locus_tag="PK977_12230"
     CDS             3900..4349
                     /locus_tag="PK977_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18932.1"
                     /translation="MSQTLFEKLQLADEKNLLIQGLPSSIEKQFSKLSFCKNMTPLLK
                     SRKIDFALVFAVSENQLNGILNDVMPSLKEGSKFWVAYPKSTSKIVTDLNRERSWNRL
                     TTVGYESIERVELDHVWSAMRFVKGQDNPRSAEVAHTRKREAAAMAE"
     gene            4412..4933
                     /locus_tag="PK977_12235"
     CDS             4412..4933
                     /locus_tag="PK977_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRF18933.1"
                     /translation="MPYLNVEIKARCTNPAFIRNYLLSNKAEYRGTDEQTDTYFHVPH
                     GRLKLREGNIENNLIYYERSNQAGPKDSHFQLVKIDDAAGLKEVLAKANGIKTVVKKK
                     REIYYIGNVKFHIDEVPGLGSFVEIEAGNILADLTKDQLKEQCDYYLAAFNIKTEDLI
                     EVSYSDMPIENLT"
     gene            complement(4926..>6771)
                     /gene="dnaG"
                     /locus_tag="PK977_12240"
     CDS             complement(4926..>6771)
                     /gene="dnaG"
                     /locus_tag="PK977_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRF18934.1"
                     /translation="SGNAIRFVMEHEKYSYVEALKWLANRYGIEIEETFQTDEQRQTQ
                     LAADSLYIINQFAQQFFTQQLFETEEGRDIGLAYFKERGFREDIIKKFQLGYSPEQRD
                     AFTREALDKQYNTELLLKTGLVALRNEQLTDNYRGRVIFPVHNHSGKVLGFGARILKN
                     NDKAPKYINTPENEIYIKSKILYGSYFARQAIDKADECLLVEGYTDVLSLHQAGIENV
                     VASGGTSLTPDQLRLIKKYTNNLTIIYDGDAAGVKAAMRGLDLALEEGLNVKLVLIPD
                     NDDPDSYVNKLGAGAFTQFVQKNKKDVILFQLEVALKDAGNDSVKKSEVVNRIAETIS
                     RINKVEDFTKQQDYIKQCAEILKIEESGLHALVNKFIRDKISTLEKKLPFEEAKTHEE
                     NIRQAEATDYDDATFNLLFKDELQEREVARILLEYGIRKWDDQKLVADYLFEEMVDES
                     LIDNADIIRLLAAFKEALQSNPESVNRNFFIYHPDTKLSTLAVSLLNFPYEESDHWRQ
                     EFSQSTGFQQNLFEQSYEDFIRTISPGNKEELMGYLKMDEDRTNEEVESAINYLKLRK
                     IKRMLLENQLDLEKEHSPDEFAILHQTHEHLKKMEMELTKKLGTVIIR"
BASE COUNT         1845 a         1388 c         1419 g         1963 t
ORIGIN      
        1 taattctaat ttcttccttt atatgagcat tccaaaattc aagaccaatg cgtccaaaaa
       61 tcattctgct aaatcctgca tataatgata atcttttgtc aaagaaaaaa taagtatatg
      121 ccggatcatc atgagtgagg tatactaacg tatctatcat tcgcacgaaa ctgtcctgca
      181 taatagcata aactgatctt atgttaatag aattaaaaac acggttattc aaaatttcaa
      241 cttttatttc gctgcctttt tttggattta tttgataaac tgcactggaa ttggttaagc
      301 catttttttt ccaaaaaatt gtagcaaaat tataaccctc aatgtaggta taaagtaaaa
      361 aaatagagtc agattgttct gagattaatg agttgtagat attttctaat gtatcaacaa
      421 atttaccatc cgaatatgct cttggaattt cgtttattat ctcaggaaaa actctgatct
      481 ggccaaaaga gagcctggca caagtaaaaa ataacagaaa taaaatattt tttttcattt
      541 cactttacac ttgaattgcc ctaaacaaac ttttatttat tctaatgtgc ttaagaatgc
      601 ccctttaaac aggtattctt tattttttaa attttcagtt acctgaaaag agatatttcc
      661 cagaaaaact tgtccttttt ggttgacttt cttgtttagt gtcagctttg catttgtcgc
      721 tctgattgag gtatgcttac catgagggat aaaaaccaca nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnngctg gcattatata aatattggat tccatcctca ttagatgaat
      961 caaccagcac tccatctaaa tacgttttgt aagcccctcc tctcacaccg ggaagtggac
     1021 cttctgaaaa tttattttcc agagttttat ctatactaaa ctcctgtttt tcttttggaa
     1081 tctgggcatc gcaggcaata aaacaaatca acaggataac tgtgaaatat ttcatttttg
     1141 gtagggtatt aaagtactgt aagtgaagga tggtgtaaat tagtcattaa ttccaaaacg
     1201 taacgttaac agcggtagct ttgcctcatg cccaatcaca ttcaaatcac ttttcatgaa
     1261 ctgcagcctg agcagcagga ggtgctgatt gcccatttgg cagaggccgg ctatgagggc
     1321 tttgagcaaa gcgataccga actcaaagca tttatccatg acagccatta cgataaggcc
     1381 ttgctgcatg aactggcttt taaataccag gtgcagtaca ccgaagagca tatcacagcg
     1441 caaaactgga atgctttatg ggaatctaat tttcagccgg tagtggtcaa tgattttgtt
     1501 ggtatcaggg cagattttca tgaaccattg caaaatgtaa gacatgagat tgtcatcacc
     1561 cctaagatga gttttggcac gggtcaccac gccactacct atatgatgat tgagcaaatg
     1621 ggggagattg atttcaaggg taaaaccata tttgattttg gtacaggtac cggggtgctg
     1681 gccatactgg ctgaaaaact aggggctgcc tatgtgctgg cgatagataa tgacgaatgg
     1741 agtatcagca atgccactga aaactgtcag cggaatgggt gccagcacat ccaattgcag
     1801 ctggcagata ccgcggccac cggcgagagc tatgatatta ttttggccaa catcaataag
     1861 aatgtaatcc tcgataacct cgtttcgctt accagccagc ttaaaaaggg aggggtgttg
     1921 ctgctgagtg gcttgctggc ggaagatgag aacgatatac tggctgccat gcagccctat
     1981 ggactaaagc ccggccaaaa atcggagaaa aacaactgga tagcattaag gttcaacggt
     2041 taagctaaaa tgaaccgtgg gttaaattaa tgttgccaga tagcattttt acctattttt
     2101 gttctccggt taagttaaaa tgattggtta ggagacgatt tcaatgaacg aacttttatt
     2161 gatcatcctt gcatacctga ttggcagcgt tcccacctcg gtttgggtca gcaaattctt
     2221 ttttgatata gacatccgtg actatggcag cggcaacgcc ggcgccacca atacgtacag
     2281 ggtactgggc cccaagtggg gtaccattgt gatggttacg gatatgctca agggtattat
     2341 tgcggtaaaa cttgctttat tattacctga atatgccgac aacgaagtga atctgcagaa
     2401 cctgcagaca ggtttgggtc tcgctgcagt tatcgggcat atcttcccga tctgggccga
     2461 ttttcgtggc ggtaaagggg tggccacatt gtttggcctg gtgctgggta tttcgccgtg
     2521 gacggcgctt agttgtgtgg gtatttttat gctggtgctg tatcttaccc gttttgtatc
     2581 cctcagttct atcctggcaa gcattgcttt tcccattttc atcctggtca tattcaatgt
     2641 agataatcct gcttaccgtg tatttgccat cgcagttgcc ttaatggtat tgctcacgca
     2701 ccagaaaaat atcggccgcc tgctgcgtgg cagtgaaagc aaagttccta ttctgaaggg
     2761 ccgtgaccgc agacgccagc gtcgtcgtga tgctgccgaa catcaggatc attgatgcaa
     2821 aaaaatctta ccttgatatt gaaataaaat gatccgtgcc aaccatggta cgatcattgt
     2881 ccgtcaattg ctaaacacat cgttatgata cgaatcattt cttccttatt ccttttatca
     2941 gcttttttaa tttcctgttc taaaaatccg ttgagcggta aaaaccagct aacgctgtta
     3001 ccagagtctg aattacagac tatggccaca ggcgaatacc agaaattttt atccaccaat
     3061 aaagtggttg ccgtttccaa caacagggat gctgccatgg tcaacagggt tggcgacagg
     3121 attgtgagag ccgttgaaac atattatgca gacaaaggca tgagtgataa attgaacggc
     3181 tttaaatggg aaaccaattt agtagaagac aaagccgtga atgcctggtg tatgcccggc
     3241 ggaaagatcg ttgtttacac cggtatactt cccatcaccc aaaatgaagc agctttggcg
     3301 gctgtaatgg ggcatgaggt atctcatgca ttacttcaac atggtaacca acgcatgagc
     3361 cagggcatta ttcagcaatt gggaggggta gccttgcagg tggcattggc caacaaaccc
     3421 caggaaacac agaacctgtt tctaacagct tatggtgtgg ggtctactgt tggggtgatg
     3481 ttgccattct cccgcaagca tgagctggaa gctgaccgat atggcctgat atggactgcc
     3541 atggcagggt ataatccaca agaagccatc ggtttatggg aaagaatgga aaaagcttcc
     3601 aatggacaaa cgccacctga gttcctgagt acacaccctt ctgaagggcg ccggatcgag
     3661 caattaaagc aatatatgcc cgaggcatta cagtattata agcctgtaaa tggcggaaaa
     3721 taaacactta atgctgatca aaaacccgcc cgaaacaacc cgggcgggtt ttttgtttac
     3781 ttaaaaccct tttaaacggg ggatttttgt tacctttgca ctccctactg cttgagaacg
     3841 ctgtaaaatg gcctcacaag gaggctgttg acaggaaccc ttaaaattag ttaccaggta
     3901 tgtcacaaac gctgtttgaa aaattacaac tggcagacga aaagaatctt ttaatccagg
     3961 gattgccctc atcgatcgaa aaacagttca gcaaattatc tttctgcaaa aacatgactc
     4021 cgttgcttaa aagccggaag atcgattttg cgttagtatt cgccgtaagc gagaaccagc
     4081 tgaatggcat tttgaacgat gtgatgccct cactgaagga aggttcaaaa ttctgggtag
     4141 cttatccaaa atccacttcc aaaatcgtta ccgacctgaa cagggaaaga agctggaacc
     4201 ggctcaccac tgtaggttat gaaagtatcg aaagagtgga attggaccat gtatggagcg
     4261 ccatgcgttt cgttaaaggt caggataacc caaggagtgc cgaagtagcc catacccgca
     4321 aacgggaggc tgctgccatg gctgaataag ccttcattcg tattcaaaat ttgttaggtt
     4381 tgttgttccc ccggaacaac aaactttttt tatgccctac ctgaatgttg agatcaaggc
     4441 ccgatgcact aatcctgctt ttatccgcaa ctatttatta tcaaataagg ccgagtacag
     4501 aggaacagat gaacaaacgg acacttattt tcatgtgccc catggacgcc tgaagctaag
     4561 ggaaggaaat attgagaaca atcttattta ctatgaacga agcaaccagg caggtccgaa
     4621 agattcacat tttcaattag tgaagatcga cgatgccgca gggctgaaag aagttttggc
     4681 aaaagccaat ggcataaaaa cggtggtaaa aaagaaaagg gagatctatt atatcggcaa
     4741 tgtaaaattt catattgatg aagtgccggg gctgggttct tttgttgaaa ttgaagcagg
     4801 taatatactg gctgatttaa ccaaggacca attaaaagag caatgtgatt attatctggc
     4861 agcatttaat ataaagactg aagatcttat tgaagtgtca tatagtgata tgcctataga
     4921 gaatcttacc tgatgataac agtgcctagc ttttttgtta gttccatctc cattttcttc
     4981 aggtgttcat gcgtctggtg cagtatggca aattcatccg ggctgtgttc tttttccagg
     5041 tcaagctggt tttccagtaa cattcgtttg atcttacgca acttgaggta gttaatagcc
     5101 gattctactt cttcattagt gcggtcttca tccattttca aataacccat cagttcttct
     5161 ttgttgccgg gagagatagt ccgtataaaa tcttcgtagg attgttcgaa aagattctgt
     5221 tgaaagccag tggactggct aaattcctgc cgccagtggt cgctttcttc ataagggaaa
     5281 ttaagcaatg ataccgcaag ggtgctcagt ttggtatccg ggtgatagat gaagaagttc
     5341 ctgtttactg actcaggatt tgattgcagc gcctctttaa atgcagccag caaacggatg
     5401 atgtcagcat tatcaataag gctttcatcc accatttctt caaacaaata gtcagccacc
     5461 agtttttgat catcccattt ccggatgccg tattccaaca gtatcctggc cacttctctt
     5521 tcctgtagtt catctttgaa caagaggttg aaagtagcat catcataatc ggtggcttct
     5581 gcctgccgga tattttcttc atgggttttt gcttcttcaa aaggcaattt tttctccaga
     5641 gttgatatct tgtcccgtat gaatttattc accagggcat gcaggccgct ttcttcgatc
     5701 tttaatatct ctgcacattg cttgatgtaa tcctgctgtt tcgtaaagtc ttctacctta
     5761 ttgatccggg aaatggtttc cgcaatccgg tttaccactt cagatttttt taccgaatca
     5821 tttcctgcat cttttaaggc tacctccaac tgaaacagga taacatcctt tttattcttt
     5881 tgtacaaatt gggtgaaggc accagctccc aatttgttta catagctgtc cgggtcatca
     5941 ttatccggta tcaggacgag ttttacattc aatccttctt ccaatgcaag gtcaagaccc
     6001 cgcatggctg cttttacacc ggcagcatcc ccatcgtaaa tgatagtaag gttattggta
     6061 tattttttaa tcagccgaag ctggtcaggt gtcagcgaag taccgccact ggctacaaca
     6121 ttttcaattc cggcttggtg cagggagagt acatccgtat atccttctac gagcaggcat
     6181 tcatctgcct tatcgattgc ctgtcgggca aaataagaac cataaagaat tttgctcttt
     6241 atataaatct cgttctccgg tgtattaatg tacttggggg ctttgtcatt gttcttcagt
     6301 atcctggcac caaagcccag cactttacca ctgtgattgt gtacgggaaa aatgaccctt
     6361 ccccggtagt tgtcggtcaa ctgttcattg cgcaaggcga caagcccggt tttcagcaag
     6421 agttctgtat tatattgttt atccaatgct tccctcgtga aagcatctct ttgttccggg
     6481 gagtaaccca actggaattt tttgataatg tcttccctga agcctctttc tttaaaatag
     6541 gccagcccga tatccctgcc ttcctctgtt tcaaacagct gctgtgtaaa aaactgctgc
     6601 gcaaactggt tgatgatata caggctgtcg gcagccagtt gtgtttgtcg ctgttcgtct
     6661 gtctggaaag tttcttcgat ctcgatacca tagcggtttg ccagccattt cagcgcttct
     6721 acatagctat atttttcatg ctccatgacg aatctgatag cattgccgct g
//
LOCUS       DAORLS010000164         6768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6085_length_6768_cov_2.213764, whole genome shotgun sequence.
ACCESSION   DAORLS010000164 DAORLS010000000
VERSION     DAORLS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_6768_cov_2.213764"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..634)
                     /locus_tag="PK977_12245"
     CDS             complement(218..634)
                     /locus_tag="PK977_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HRF18935.1"
                     /translation="MYPEEIVIPMKEELTENGFTELLSPAEVEAQLAKEGTTLVMINS
                     VCGCSAGTARPGVLMAVHNTPKKPDYLTTSFAGFDVDAVKALRQHLMPYPPSSPAIAL
                     FKNGQLVHFIERHQIEGRPDQVIAQNLIGAFEQHCN"
     gene            856..1302
                     /locus_tag="PK977_12250"
     CDS             856..1302
                     /locus_tag="PK977_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF18936.1"
                     /translation="MAYVKFSRKPFEGTFSNLVDDLFTELPVLLSSGFNNTVRKDSVP
                     VNVKETEKSFQLDVIAPGFDKTDFKVNIEQNILTVSAEKTNEANEESDKQIRKEYSFR
                     SFKRSFTLDEKIDATNIEASYVNGVLRLNLPKKEAVKQSATEIVIK"
     gene            complement(1394..1846)
                     /locus_tag="PK977_12255"
     CDS             complement(1394..1846)
                     /locus_tag="PK977_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF18937.1"
                     /translation="MRYFLIVLTMISCGSSANSQTAEDSVKSVINKMFVGMREADTAM
                     IKTCFADSMIMQTIFRNKEGNLIVRNESPSGFISFIAKEAKGNADERISFETVKVDGP
                     LAMAWTPYIFYYKGQFSHCGVNSFQLVRFNGEWKIQYLVDTRRRQGCK"
     gene            1930..>2126
                     /locus_tag="PK977_12260"
     CDS             1930..>2126
                     /locus_tag="PK977_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18938.1"
                     /translation="MQTARLVQAVIMMLIVALAASCAATKEYSSKLFAPRTTMQKDSQ
                     SLALRFLEIDNNEKNTEDWVTT"
     assembly_gap    2127..2136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2137..2358
                     /locus_tag="PK977_12265"
     CDS             <2137..2358
                     /locus_tag="PK977_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18939.1"
                     /translation="IINGADTAISTASLDNLAKILPSETKKDSLQAKNSEKTETPIAA
                     ETKTNKPTDEPVARTTNTNGIRSKKTREE"
     gene            2410..2796
                     /locus_tag="PK977_12270"
     CDS             2410..2796
                     /locus_tag="PK977_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18940.1"
                     /translation="MKRTFILTTVTITILALVLTSCKSSRVWETRDRTERTSRKKHPP
                     PPPAAAPRYYSSVALIINPTPGFTMNRYHDGRYFHRSPGGLLYWKGYDNRFFLDGSYL
                     NRISYSKWQYDEWRRYKRASESNRRR"
     gene            complement(2856..4256)
                     /locus_tag="PK977_12275"
     CDS             complement(2856..4256)
                     /locus_tag="PK977_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRF18941.1"
                     /translation="MTNSYHDLVKQTFNFPQEGIEVKEGDLFFNGLDLNALIEKYGTP
                     MKLTYLPKIGMQIKKAKQMFAAAFKKHKYEGKYFYCYCTKSSHFSFVVEEALKNDIHL
                     ETSYAYDIEIIKKLYQKKKITKDIHIICNGFKQKGYTRRIANLINSGFSNVIPVLDNK
                     EELKDYSSTVKVPFKLGIRVAAEEEPSFPFYTSRLGIRAKDILEFYVDRIEGYESKFQ
                     LKMLHIFLNKGIKDDIYYWSELNKVINLYCQLKKICPELDSINIGGGFPIKHSLGFEY
                     DYQFMINEIVRNIKTACKKAKVPMPNIFTEFGSYTVGESMAHIYKVIAEKVQNDRETW
                     YMIDSSFITTLPDTWGIGEKFLMLPVNKWDHDYQRVVLGGITCDSHDYYDSEEHINEV
                     FLPKTNGDKEPLYVGFFHTGAYQDQISGYGGIKHCLIPSPKHVIVGYDKNGKLVDWVY
                     AKEQSAQSMLKILGYK"
     gene            complement(4357..5262)
                     /locus_tag="PK977_12280"
     CDS             complement(4357..5262)
                     /locus_tag="PK977_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRF18942.1"
                     /translation="MKKFGAGLLFSLLTLAAWSQKDSDVNTLLWKISGNGLERPSYLY
                     GTIHMICAEDAGLSNNFKKIIKECDEVYFEVDMDNLFEMIGAMGKMKMKGDTTLRDLL
                     NENDYQKVKDYFEKKGSMLPFSMLETYKPILAASTLEQRSLDCDNTAVMEQVIMEEAQ
                     KSNKKINGLETMSYQAGVLDSIPYKLQAMQLVSYIDSATSGDSDNQQLNELFQAYKSQ
                     DLKKLEELMVESDAGMSGFTDILLYHRNLNWVKKLNQLLPDKSLVIAVGAGHLPGEKG
                     VINLLRKNGYKVTPVENKITRIREI"
     gene            complement(5389..>6014)
                     /locus_tag="PK977_12285"
     CDS             complement(5389..>6014)
                     /locus_tag="PK977_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF18943.1"
                     /translation="VEQFQNAVAELGHPSKQVCFKPSVSNGSRGFRIISDQFNEADIL
                     FNQKPNNVFIRLNDVLRVLSSAPFPELLVSQYLPGDEFSVDCLCNHGKALLVLPRLRK
                     KMVNGISVKGEFVKKDQIIDYCTRLIKELQLHGNIGIQVKADQEGEFLVLEINPRVQG
                     TISAALGAGVNLPLLAVKQELGLPIMPYELFVKWGVRFSRYWEEVFY"
     assembly_gap    6015..6037
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6038..6444)
                     /locus_tag="PK977_12290"
     CDS             complement(<6038..6444)
                     /locus_tag="PK977_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18944.1"
                     /translation="MSRELINVLMSGAGAPGAAGILKCLQQDKSVHVICADADPDATG
                     KYLANDFVSIPAAGKSDFTDKVLSICREKNIHVLMPLVTKELIPLSQHIKEFELAGTK
                     LLVSSTASLEIANNKSRLYEFLQWRGLKIPNFRV"
     gene            complement(6480..>6768)
                     /locus_tag="PK977_12295"
     CDS             complement(6480..>6768)
                     /locus_tag="PK977_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HRF18945.1"
                     /translation="ATALWRFCNSLDPKRDFYLYKSAQLPYTACMGLDGTRKTKEFDN
                     FQRDWPNVIVADNETIKSVDEKWSQLGLGAFIPSPSLKFMNQIYGPEAVVN"
BASE COUNT         2053 a         1450 c         1207 g         2025 t
ORIGIN      
        1 aaagccgaat gaattaacga agcgaagaaa ggtccattct acgccaaaaa ggtccttaaa
       61 agcatagtaa agattaggat acatgaatac ttaattggtt gggggcgaat atactaaaga
      121 aagccccgag ttatcatttt tatcctttaa gatttgtatt aaaaaaagcc ccgaataata
      181 tccggggcca tcaacaaatt attttgaaat gtaggtatta gttgcaatgt tgctcaaatg
      241 ctccaatcaa gttctgggct attacctggt ccggtctgcc ttcaatctgg tgcctctcga
      301 taaaatgtac cagttgtccg ttcttaaaaa gagcgatggc cggtgatgaa ggaggatatg
      361 gcataagatg ctggcgcaac gctttgactg catctacatc aaaaccagca aaactggtgg
      421 taagataatc aggcttcttg ggagtattat gcaccgccat taaaacaccg gggcgggcag
      481 taccggctga acatccgcaa acggaattga tcataacaag ggttgttccc tctttagcga
      541 gttgtgcctc tacttctgca ggcgaaagaa gttctgtaaa tccattttca gtcagttctt
      601 ctttcatggg gattactatt tcctctggat acataattta tctgtttgat ttttaataaa
      661 acacaaagct atacaaaatg ttggggtgtt attattgata cctcgggcag aagtaattct
      721 ggcagcaaaa atttctgatt tcggaaatta tgtccccttt tattcatccg tactgccagc
      781 tatgacagaa aaagaggtca tggtatgggg tttgtcgaac tctgttcgta ttcattaatc
      841 taaaaactat acactatggc atacgtaaaa ttcagcagaa aaccttttga aggcacattt
      901 agcaatcttg tcgatgacct ctttacagaa ctgccggtct tgttaagcag cggctttaac
      961 aatactgtaa gaaaagattc agttcctgta aacgtcaaag aaacagaaaa gagttttcaa
     1021 cttgatgtga tagcaccggg ttttgacaaa actgatttta aagtaaacat cgaacaaaat
     1081 atcctgacgg tctctgccga aaagacaaat gaggcaaatg aagaaagtga taaacaaata
     1141 aggaaggagt acagcttccg ttcttttaag agatctttca cattggacga aaaaattgat
     1201 gcaacgaata ttgaagcatc ctacgtcaat ggtgtgttga ggctaaacct tccaaaaaag
     1261 gaagctgtca aacaatcggc gactgaaatt gtaataaaat aagttcttac ataaaagttt
     1321 tctcataagc agttggtttt ggtttacacc ctccgtttct acggagggtt tcttttttta
     1381 tacctggcaa ctattattta caaccttgtc tccttctggt atcaaccagg tattgaattt
     1441 tccattctcc attaaatctt accaactgaa aagaatttac gccgcaatgg ctgaactgtc
     1501 ctttgtagta aaaaatgtac ggagtccagg ccattgctaa agggccatca acttttactg
     1561 tttcaaaact tatcctttca tcggcattcc cttttgcttc tttggcaata aaactgatga
     1621 acccagaggg tgattcattt cgcacaatca aattcccttc tttgttcctg aatatcgtct
     1681 gcataatcat actgtccgca aaacaggttt ttatcattgc agtatcggct tcccgcatac
     1741 caacaaacat cttattgatt acactcttca cagaatcttc tgctgtctgt gaatttgctg
     1801 agcttccaca agaaatcatt gttaagacta tcaaaaagta tcgcataaaa aaaggtttta
     1861 agactttatg gcaaatgaat tggttgataa atacgtttag taacctttcc aaatataaaa
     1921 ctacatgcca tgcaaacagc cagattggta caagcggtaa taatgatgtt gattgtagca
     1981 ttggccgcca gttgcgccgc tacaaaagaa tatagcagta agctatttgc accaagaaca
     2041 acaatgcaaa aagatagtca atcacttgcc ctccgatttc ttgaaattga caacaatgaa
     2101 aaaaatacgg aagattgggt aacaacnnnn nnnnnnatca ttaatggcgc cgacactgca
     2161 ataagtactg cttcacttga caacctggca aaaattcttc catctgaaac caaaaaagat
     2221 tccctacagg ctaaaaactc agagaagaca gaaacgccta tcgcagcaga aacaaaaaca
     2281 aataaaccta cagatgagcc tgtggccagg actaccaata ctaatggaat aagatcaaaa
     2341 aagacaagag aagaataacc aatttatcaa ataaataaac cgtatctcct aaccatcaaa
     2401 caaatagtca tgaaaagaac atttattctt acaacggtaa caattactat tcttgctttg
     2461 gttttaacca gttgtaaatc cagccgggtt tgggaaacca gggacagaac agaaagaact
     2521 tccagaaaaa agcacccgcc acctcctcct gcagcagcac cccgctatta cagttctgta
     2581 gccttgatca taaatccaac tcccgggttc acaatgaacc gatatcatga cggaaggtat
     2641 tttcatcgta gtccgggtgg cttactttat tggaaaggtt acgacaatcg tttcttcctt
     2701 gacggatctt atctgaaccg gatcagctat tcaaaatggc agtatgacga gtggagacga
     2761 tataagagag catctgaaag taatcgtaga agatagtaaa caaacaatag taggccccat
     2821 tgccggcaga cataaaatac agcgggcaat ggggtctatt tatatccaag gattttcaac
     2881 atactttgtg cagattgttc tttggcatat acccagtcaa cgagtttgcc gtttttatca
     2941 tagcctacta tcacatgctt gggcgatgga ataagacaat gtttaatacc tccataaccg
     3001 ctgatctgat cctggtaggc gccagtatga aaaaagccaa cgtataacgg ctctttatct
     3061 ccattcgttt taggcagaaa tacttcgtta atatgttctt ccgagtcata ataatcatga
     3121 ctatcacagg taataccacc taataccacc cgttggtaat catgatccca tttattaacc
     3181 ggcagcatca ggaatttctc tcctatcccc catgtatccg gtaaagtagt aataaaagaa
     3241 gaatcgatca tataccaggt ttctctgtcg ttttgaactt tctctgcaat tactttataa
     3301 atgtgagcca tactctcccc tacggtatag ctgccaaatt ctgtaaagat attcggcatc
     3361 ggcactttgg cctttttaca tgctgttttg atatttctta caatttcatt gatcatgaac
     3421 tggtaatcat attcaaaccc gagagaatgc tttatcggga acccccctcc tatatttatt
     3481 gaatccagtt cagggcagat tttttttagc tggcaataaa ggttaataac cttgttcagt
     3541 tcactccagt aataaatatc atctttaatc cctttattca ggaaaatatg cagcatcttc
     3601 aattgaaact tgctttcata tccttcgatc ctatccacat aaaactcgag tatatcctta
     3661 gccctgatcc ccagcctgga tgtataaaaa ggaaaacttg gttcttcctc agcagccacc
     3721 cttatcccta acttaaaggg aacctttaca gtggaagaat agtcttttaa ttcttcctta
     3781 ttatccaaca ccggtataac attagaaaag cctgagttaa tcaggttagc tattctcctt
     3841 gtgtaacctt tttgtttaaa tccattacaa atgatgtgta tatcctttgt tattttcttt
     3901 ttctggtata gctttttgat gatctcaatg tcataagcat aggatgtttc aagatgtatg
     3961 tcgtttttaa gggcctcctc aactacaaag ctgaaatgag aacttttcgt acagtagcag
     4021 tagaaatatt ttccctcgta tttatgtttt ttaaatgctg cagcaaacat ttgtttggcc
     4081 ttctttatct gcatccctat tttgggcagg taagtaagtt tcattggggt gccatacttc
     4141 tcaatcaggg cattcaggtc aagcccgtta aagaacaaat caccctcttt tacttcaata
     4201 ccttcctgtg gaaaattaaa tgtttgctta acgaggtcgt ggtaagaatt agtcatgtaa
     4261 gccagaatgg gttaggccat aaattatttt gacggcacaa atgtataaga ttggtgaaat
     4321 tgaaggccaa aaatttatgc ccccggatct accagatcag atttccctta tcctggtaat
     4381 cttgttttca acgggtgtta ccttgtagcc attcttacga agcaggttta ttaccccctt
     4441 ttcacccggt aaatgcccgg ctccaactgc aatgaccagt gatttatcgg gtaataattg
     4501 attcagcttt ttaacccaat tgaggtttcg gtggtaaagt aagatatctg taaacccaga
     4561 catacctgca tctgattcta ccataagttc ctccagtttt ttcaggtcct ggcttttgta
     4621 agcctgaaaa agctcattaa gctgctggtt atcgctatca ccagacgtag cactgtcaat
     4681 ataactcacc agctgcattg cctgtaattt gtacggaata ctatcgagta ctccagcctg
     4741 gtacgacatc gtctcaaggc cgtttatttt cttattgctt ttttgggcct cttccattat
     4801 tacctgctcc ataacagccg tattatcaca atccaatgaa cgctgctcca atgttgaagc
     4861 agccagaatg ggtttataag tctccagcat ggagaaaggg agcattgatc cttttttctc
     4921 aaagtaatct tttacttttt ggtagtcatt ttcgtttagc aagtctctta atgtagtatc
     4981 acctttcatt ttcatcttgc ccatagcgcc aatcatctca aacaggttat ccatatccac
     5041 ttcgaaataa acctcatcac attccttaat tattttttta aaattattac taaggccagc
     5101 atcctccgca caaatcatat ggatggttcc atacaggtaa gaaggtctct caagcccatt
     5161 gccgcttatc ttccacaaaa gtgtattcac atcgctgtct ttctgactcc aagccgctag
     5221 tgtcagaagg ctaaaaagta gtccggctcc aaattttttc attcaatttt ttttaagcaa
     5281 attaaaagag tatctccgta tttccggggt tgcatgactg tcccgccagc gaaatgaata
     5341 aacaagaatc tttccggatt cctgattatg tctttgtgac agaaaaattc aataaaaaac
     5401 ttcttcccaa taccttgaaa acctgacgcc ccattttaca aatagttcat atggcataat
     5461 tggcagcccc agttcctgct taacagccag caaaggcagg tttactccag cccccaaagc
     5521 ggcagaaatg gttccttgta cccggggatt tatttccagc accagaaatt caccctcttg
     5581 atcagctttc acctggattc caatgtttcc atgaagttgc aactctttaa tgagccgggt
     5641 gcaatagtca attatctgat ctttttttac aaattcaccc tttacagaaa tgccattaac
     5701 catttttttt cgaagccgtg gtaacaccag caaagctttc ccgtgattgc aaaggcaatc
     5761 aaccgaaaat tcatcaccgg gcagatattg actaaccagt aattccggaa aaggggcaga
     5821 tgagagaact ctcaatacat catttagcct tatgaaaaca ttattgggct tttgattaaa
     5881 taagatatct gcttcattga attgatctga gatgatccga aagcccctgc taccgtttga
     5941 aacagatggc ttaaaacaaa cttgttttga agggtgaccc aattctgcta cagcattttg
     6001 gaattgttca acatnnnnnn nnnnnnnnnn nnnnnnnacc ctgaaatttg gaattttcaa
     6061 tccccgccat tgaagaaact cgtatagccg gcttttatta tttgcgatct ccagcgaagc
     6121 agtagatgaa acaagcaatt ttgtccctgc cagttcaaac tcttttatat gctgtgatag
     6181 tggtattagt tctttcgtca ccaacggcat caggacatga atgttttttt ccctacaaat
     6241 agaaagaacc ttatccgtaa aatcagactt acccgctgcc gggatgctaa caaaatcatt
     6301 tgccaggtat ttgcctgtcg catcaggatc agcatcggca catataacat gaacgctttt
     6361 atcttgctga agacatttta aaatacctgc agcaccgggt gcgccggcac ctgacatcaa
     6421 aacattgatt aattctctgc tcacttgcta aaagtaaata atatccggga tgaatcagct
     6481 taattaacca ctgcttccgg gccatatatt tgattcatga actttagaga cggcgatgga
     6541 ataaaagccc ccaatcccaa ttggctccat ttctcatcca cggatttaat cgtttcatta
     6601 tctgcaacaa taacattggg ccaatccctt tgaaaattgt caaactcttt tgtctttctg
     6661 gttccgtcaa gccccataca agcggtgtag ggcaattgcg ccgatttgta taaataaaag
     6721 tcacgtttag gatcaaggct attacaaaac ctccataagg ccgtagcc
//
LOCUS       DAORLS010000165         6739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6120_length_6739_cov_2.581837, whole genome shotgun sequence.
ACCESSION   DAORLS010000165 DAORLS010000000
VERSION     DAORLS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_6739_cov_2.581837"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PK977_12300"
     CDS             <1..983
                     /locus_tag="PK977_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF18946.1"
                     /translation="LNFIFSANSFNDAVKRISYLKSYKDYRERQVNTIQETQQLIAAR
                     KKQQLSRKDQKNVALQNQAKQVQELSVQKKEKDVVVAQLKSKQKDLEKQIAAKKKKDR
                     DLQNAILAIVRREIKAAEDAAKAKAKAEADAKKKDEELAKKNNTVVKPETNPAPTGTT
                     PTTTTAVTKRNEPTSRPASYLDLNAKDVALNSSFAANRAKLPWPVDNGVVTLRFGSNK
                     IENTLLTFDNPGITISTPSTGVAVKAVFDGEVSGVYNLGDGMVVTIRHGKYFTTYSNL
                     SGVTVTKGSVVKTGQQIGKAGKDDEGSGGQVDFILMIETKNVDPLPWLRR"
     gene            complement(1035..2156)
                     /gene="bshA"
                     /locus_tag="PK977_12305"
     CDS             complement(1035..2156)
                     /gene="bshA"
                     /locus_tag="PK977_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRF18947.1"
                     /translation="MKIGIVCYPTFGGSGVLATELGKALAQKGHMVHFITYQQPVRLN
                     EFMPNIFYHEVQVPTYPLFDYPPYETALASTMVDVIKNNNLDLLHVHYAIPHASAAYM
                     AKQILKKEGKNIPVITTLHGTDITLVGRDKTYAPVVTFSINESDGITAVSQNLRDETY
                     KHFQIEKNIEVIVNFVDVSRFSRKPLDAFKKVIAPNGERILLHASNFRKIKRVQDVVK
                     IFYEVQKQIPSKLLFVGDGPERSTAEDLSRELGVCDEIRFVGKQEQMEDILAIGDLFL
                     LTSEYESFGLAALEAMAAGVPVVSTNAGGLKEIIVQGETGFMSDVGDIDSMSKQALSI
                     LSDNKTLQSFKEKAAAQARKFDIHNIIPVYEKLYERFLK"
     gene            2271..3674
                     /locus_tag="PK977_12310"
     CDS             2271..3674
                     /locus_tag="PK977_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF18948.1"
                     /translation="MRKFTFLLLAVISSTTIFSQNEDSIQIKRISDEILSNGKAYENL
                     RYLTKKIGPRLAGSQGMVKSEQWGLKMMQESGADMAWMQECKVPHWVRGGKDAANAAY
                     IPSRSKVKKGPITATIKKELDIVALGNSVGTGNKPLTAEVLLVHSFDELERRKDEVKG
                     KIVFYNYKFNPTYVNTFLSYRDAGQYRGQGPSRASKYGAKAVIVRSLSHAADNHPHTG
                     ATRYDSAYAKIPAVAIGLRDADWLSEQLQEGTIKVTLITNGHFLPDTIGHNIIGELKG
                     TEFPDEIITVGGHLDSWDNCEGAHDDGAGCVQTIEILRAFKAIGYKPKRTIRFVLFAN
                     EENGLRGGNKYAEEAKAKNEKHLLAMESDAGGFTPRAIGFSGSDEQYAKVLSWKSLIA
                     PYGCTEINKGGGGADIGPLNRALKTPTASLNPDTQRYFDIHHARSDVFEAVNKRELEL
                     GAVNMAAFIYLVDKYGL"
     gene            complement(3678..>4454)
                     /locus_tag="PK977_12315"
     CDS             complement(3678..>4454)
                     /locus_tag="PK977_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRF18949.1"
                     /translation="YDVVNKNPLGSAAGYGSSFPINRTLTTQLLGFEDLNYNVVYAQM
                     GRGKAERIVAQVLTNVADTLSKLSMDACLYLNQNFGFISFPAELTTGSSIMPHKKNPD
                     VFELIRSHCNRIKALPNEIMLITTNLPSGYHRDLQLLKEHLFPAFKTIKDCIEMAGLM
                     ISNIDVKENILADEKYKYLFSVEEVNKLVNKGIPFRDAYKKIGLDIEAEKFTYDTSIN
                     HSHEGSIGNLCTDEIKKQMQKVINSFPFTSVHQAITSLLK"
     assembly_gap    4455..4464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4465..>4842)
                     /locus_tag="PK977_12320"
     CDS             complement(<4465..>4842)
                     /locus_tag="PK977_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRF18950.1"
                     /translation="LQTELRSIYRQIQKEEFVLQENVEDIHSQVELLLTQKLGDTGKK
                     IHSARSRNDQVLVDIRLFLRNELEELVKVIQPFFELLQSQSEKYKNHLLPGYTHLQLA
                     MPSSFGLWFGAYAESLVDDVITI"
     assembly_gap    4843..4860
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5012..>5103)
                     /locus_tag="PK977_12325"
     CDS             complement(5012..>5103)
                     /locus_tag="PK977_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18951.1"
                     /translation="RSHTADEYIYIEEIRTGIEIYIQLLNNVV"
     assembly_gap    5104..5113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5114..6091)
                     /locus_tag="PK977_12330"
     CDS             complement(<5114..6091)
                     /locus_tag="PK977_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRF18952.1"
                     /translation="MNSDLNILYIDAIGLLKELIATPSFSKEEEKTAGIIARFFASRD
                     IAATWVGNNIFVLNRYFDEKKPTILLNSHHDTVKPNPQYTKDPFSPIEEEGKLFGLGS
                     NDAGGCLVSLIATFLYFYDRPDLKYNIIIAATAEEEISGTGGIEYTLKYLPKIDSAIV
                     GEPTLMQMAVAERGLMVLDCISHGKAGHAARNEGENAIYKALKDIEWFRNYQFTKVSE
                     LLGPSRMNVTVIETENKAHNVVPAVCKFIVDTRINELYSFEEVLDIIKANVNCEVKPR
                     STRLRSTSIPLDHPLVKAGLDLGRSYYGSPTTSDKALMPFPALKIGPGDS"
     gene            complement(6084..>6739)
                     /gene="argB"
                     /locus_tag="PK977_12335"
     CDS             complement(6084..>6739)
                     /gene="argB"
                     /locus_tag="PK977_12335"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRF18953.1"
                     /translation="TKMAEKLGIEQQLVDGRRITDAETLKIVTMVYAGFINKNIVAAL
                     QSKNCNAIGICGADGDVILAHKRQHPVLDYGFVGDVDAINTDLISTLLNKNIAIVFAP
                     ITHDQQGQLLNTNADTIAQEVAKGMSSIFDVDLIYSFEKTGVLLDANDDSTVIPEINS
                     SYYQQLKAKNLIFAGMIPKLDNSFAALKSGVGKVIIGKAENLPQLLDGSAGTSIVNE"
BASE COUNT         2015 a         1306 c         1376 g         2004 t
ORIGIN      
        1 atctcaattt tatcttttca gccaatagtt tcaatgatgc ggtaaagagg atatcatacc
       61 ttaaaagcta taaagattac agggaaaggc aggtgaatac aatacaggaa acccagcagt
      121 tgattgcggc aagaaaaaaa cagcaactgt caagaaagga tcagaagaat gttgcgttgc
      181 aaaaccaggc taagcaggtg caggaactgt ccgttcaaaa gaaagaaaaa gatgtggtag
      241 ttgcccaatt gaagtcaaag caaaaagacc tggaaaagca aattgcagca aaaaagaaaa
      301 aggacaggga ccttcagaat gctattctgg ccatagtaag aagagagata aaagcggctg
      361 aagatgcagc aaaagccaag gctaaggcag aagcggatgc caaaaagaag gatgaggagc
      421 tggctaagaa aaacaataca gttgtaaaac ccgaaacaaa tcctgcacct acgggaacaa
      481 cgccaactac tacgactgcc gttacaaaaa gaaatgaacc tactagcaga ccggcaagct
      541 atcttgattt gaatgcaaaa gatgttgcac tcaacagtag ttttgcagcc aacagagcta
      601 agttgccgtg gccagttgat aatggtgtag ttactcttcg ttttggctcc aataagattg
      661 aaaatacact gctgactttc gataatcccg gtattacaat ttcaacgcct tctaccggtg
      721 tggctgtgaa agctgttttt gacggagaag tcagtggcgt ttataacctt ggagacggaa
      781 tggtggtaac catcaggcat ggtaaatatt ttacaaccta tagtaactta tctggtgtaa
      841 ctgttacaaa gggttctgtt gttaaaacag ggcaacagat aggtaaggct ggtaaagatg
      901 atgaaggtag tggtggccag gtggatttta ttttgatgat tgaaacgaaa aatgttgatc
      961 ctttgccttg gttgagaaga taagtatttt gatattatac aactaaaaag acccggttta
     1021 accaggtctt ttttttattt cagaaatcgt tcatatagct tttcgtaaac cggaataata
     1081 ttatgtatgt cgaatttcct ggcttgtgcc gcagcttttt ctttaaaaga ctgaagggtt
     1141 ttgttgtcac ttaatatact aagggcctgc ttactcatag agtctatatc tccaacatca
     1201 ctcatgaaac ccgtttctcc ctgtacaatt atctctttca agccaccggc atttgtagaa
     1261 acgactggaa ctccggcggc cattgcttcc aaagctgcta aaccgaagct ttcatattca
     1321 gaggtaagca gaaacagatc gccaatagcc aaaatgtctt ccatttgctc ttgtttacct
     1381 acaaatctta tttcatcaca tacacctaat tcacggctca ggtcttctgc agtacttctc
     1441 tccgggccgt cgcccacaaa taaaagttta gaggggattt gcttttgtac ttcatagaag
     1501 atcttaacaa cgtcctgcac tctctttatt tttcggaaat tagaggcatg taataaaatc
     1561 cgttcaccgt ttggagcaat aactttttta aaagcatcca gtggctttct gctgaaacgg
     1621 ctgacatcaa caaaattgac aattacctca atattttttt ctatctggaa atgcttatat
     1681 gtctcatccc gcaggttttg tgatactgca gttatgccat cactttcgtt tatagaaaag
     1741 gttaccacag gtgcataggt tttatccctg cctaccagtg ttatatctgt tccgtgcaat
     1801 gttgtgatta caggaatatt ttttccctct ttctttaata tctgtttggc catatacgcc
     1861 gcagaggcat gaggaatagc gtaatgtaca tgtagcagat ccaggttatt attctttatc
     1921 acatcgacca tggtactggc taatgcggtt tcgtaagggg gataatcaaa taatggatag
     1981 gttgggactt gcacctcatg ataaaatata ttcggcatga attcattaag ccgaaccggc
     2041 tgctgataag taatgaaatg aaccatatgc cctttctggg ccagggcttt acccagttct
     2101 gtagcaagta ctcccgatcc gccaaatgta ggataacaaa caattccgat tttcatacta
     2161 ttcctttaag ggacatctaa ggtaaaggat ttgtatgggt ttggtttgcc gcttatctta
     2221 aataattatc ttcatttttt atttctggta attttagcaa aattcggtac atgagaaaat
     2281 ttacatttct attactggca gttatttcct caactacgat tttttcacaa aatgaggatt
     2341 caattcagat caagcgaatt tcagatgaga tactcagtaa tggtaaggca tatgagaatc
     2401 tgcgttacct gacaaaaaag atcggtccga ggctggccgg atcacaggga atggtaaaat
     2461 cggaacaatg gggtttaaaa atgatgcagg aaagcggtgc tgacatggcc tggatgcagg
     2521 aatgtaaggt gccacactgg gtcagaggag gaaaagatgc tgccaatgcc gcatacatac
     2581 catccaggtc caaagtaaag aaaggcccca ttacagcaac gattaaaaaa gaactggata
     2641 tagttgccct cggtaactct gttggcacgg gtaacaaacc gcttacagca gaggtattgt
     2701 tagttcattc atttgatgaa ttggagagaa gaaaagatga ggtaaagggc aaaatagttt
     2761 tttataacta taagtttaac ccaacctacg taaatacttt tctttcttac cgtgatgccg
     2821 gtcaataccg ggggcaaggc cccagccggg catcaaaata tggagcaaag gcagttatcg
     2881 taagaagtct aagtcatgca gcagataatc atccgcatac cggtgcaaca agatatgata
     2941 gtgcttatgc aaaaattccg gcagtagcta ttggcttaag agatgcagac tggctgagtg
     3001 aacagcttca agagggaact ataaaagtta cattaataac aaacggacat tttttacctg
     3061 atacaattgg ccataacata attggggagt taaaagggac agagtttccg gatgaaatta
     3121 taactgtggg tggacacctt gatagttggg ataactgcga aggtgcacat gatgacggag
     3181 ccggatgtgt acagacaatt gaaatattaa gagcctttaa ggctatcggt tataaaccta
     3241 aaagaacaat ccgtttcgtt ttgtttgcca atgaagaaaa tggtttaagg ggaggtaata
     3301 aatatgctga agaagccaag gcaaaaaacg aaaagcattt gctggctatg gaaagtgatg
     3361 caggcggttt tactccaagg gcaattggtt tttcaggtag tgatgagcag tatgcaaaag
     3421 tattatcctg gaaatccctg attgctcctt atggctgcac agaaatcaat aaaggcggtg
     3481 gaggggcaga catcggacca ttaaaccggg cattgaaaac accaacagcc agtttaaatc
     3541 ctgatacgca gcgttatttt gatattcatc atgccagaag tgatgtgttt gaggcagtaa
     3601 ataagagaga gttagaactc ggagctgtga atatggcggc gtttatctac ctggtagata
     3661 aatacggctt gtaactccta tttaaggagg ctggtaattg cctgatgaac agatgtaaaa
     3721 ggaaatgagt taatcacttt ttgcatctgt ttttttattt catctgtaca aagattgcca
     3781 atactgccct catgggaatg gttaatagaa gtatcataag tgaatttttc tgcttctata
     3841 tcaagtccaa tttttttata agcatccctg aaaggaattc ctttatttac cagtttattt
     3901 acctcttcta cactaaagag atatttatac ttttcgtctg ctagaatatt ttctttcacg
     3961 tcaatattgg aaatcattag ccctgccatt tcaatacaat cttttatggt tttgaaagcc
     4021 ggaaataaat gctccttcag caactgcaaa tcccggtgat aaccagatgg caagttagtt
     4081 gtaatcagca ttatttcatt gggcagtgct ttgattcggt tacaatgaga acggattaat
     4141 tcaaatacat ccgggttctt tttatgtggc attatgctgc tgcctgttgt cagttcagcc
     4201 ggaaaagata tgaaaccaaa gttctggttc aggtacaaac aggcatccat acttagtttt
     4261 gataaagtat ctgcaacatt ggtaagtact tgtgctacga tgcgttctgc ttttcctctt
     4321 cccatttgtg catagactac gttatagttc aggtcttcaa aacccagcaa ttgagtggtc
     4381 agtgttctgt tgatagggaa ggaggagcca tagccggcgg cagatcccaa cgggttctta
     4441 tttaccacat catannnnnn nnnntgatgg tgataacatc atcaaccagg ctttctgcat
     4501 aagcaccgaa ccaaagtccg aaagaagagg gcatagccaa ttgtaaatgt gtgtaaccag
     4561 gtaataaatg atttttatat ttttcactct ggctttgcag caattcaaag aatggctgaa
     4621 ttacttttac caattcttcc agctcattcc ggagaaataa cctgatatca accaatacct
     4681 ggtcgttgcg gcttcgggct gaatggattt tctttcctgt atctcccagt ttttgagtca
     4741 gtaacaattc tacctgcgaa tgaatatctt ctacattttc ctgcagtaca aattcttctt
     4801 tttgtatttg ccggtatatt gatcttaatt ccgtttgtaa ttnnnnnnnn nnnnnnnnnn
     4861 tttgtcaata atccaactga ctccagcatt tcaatatgcg ccaaagatcc taatacatca
     4921 aaagcagcca ggtacatatc catttcacgg tctttgccga cagtgaattc ttctacctgc
     4981 ttcaatgatg atttgtcttt ttgccaaagc ttcatactac gttatttaat agctgaatgt
     5041 aaatttcgat accggtcctt atttcctcaa tataaatata ctcatcggct gtgtggctgc
     5101 gggnnnnnnn nnnagaatct ccaggtccta tttttagtgc tggaaaaggc attaatgcct
     5161 tatcagaagt ggtaggagag ccatagtaac ttcttcccag gtccaagcca gccttaacca
     5221 gcgggtgatc caatggaata gaagtggacc taagccgggt actcctgggt tttacttcac
     5281 aattaacatt cgctttaatt atgtcaagta cctcttcaaa actgtacagt tcattgatcc
     5341 ttgtatcaac aataaattta caaacagccg gaacaacatt gtgtgctttg ttctctgttt
     5401 caataactgt tacattcatc ctggaaggtc ccagcagttc tgaaacttta gtaaactggt
     5461 aattcctgaa ccattcaata tctttcagtg ctttataaat ggcattctct ccttcatttc
     5521 ttgctgcatg accagcttta ccatgactga tacaatcaag caccatcagg cccctttcag
     5581 caaccgccat ttgcattagt gtgggttcac ctacaatggc agaatcaatt ttaggcaagt
     5641 actttaatgt atactcaatt cccccggtgc cgctaatttc ttcttcggct gtagcagcaa
     5701 ttataatgtt atattttaaa tcaggccggt catagaaata caaaaaagtt gctataaggg
     5761 atacgaggca accacctgca tcattactac cgaggccaaa cagttttccc tcttcttcaa
     5821 tcggtgaaaa aggatctttg gtgtactgcg gattaggctt taccgtatca tggtgtgaat
     5881 taaggagaat ggttggtttt ttctcatcga agtacctgtt caatacaaat atgttattac
     5941 caacccaggt agcagctata tctcttgagg caaaaaatct ggcgataatg cctgcagtct
     6001 tttcttcttc cttactgaaa gaaggagttg caattaattc ttttaaaagc ccaatagcat
     6061 ctatgtataa tatgttcagg tcgctattca tttacaatac ttgttcctgc gcttccgtcc
     6121 aatagctgtg gaagattttc tgctttgcca atgattactt ttcctacacc acttttaagt
     6181 gcagcaaagg aattgtcaag ctttggtatc ataccggcaa agattagatt ttttgctttt
     6241 agttgctggt agtaagatga gttgatttct ggaattacgg ttgagtcatc gtttgcatcc
     6301 agcaagacac cagttttttc aaaggagtaa attaaatcaa catcaaaaat gctgctcatt
     6361 cctttggcaa cttcctgggc aatagtgtca gcattggtgt ttaataactg cccttgctga
     6421 tcatgagtga tgggagcaaa tacgattgcg atgtttttgt tcagcaaagt gctgattaga
     6481 tctgtattaa tagcatccac atcccccaca aaaccataat caagaacagg gtgttgtcgt
     6541 ttgtgagcta aaattacatc accatcagca ccacaaattc cgatggcatt acaatttttg
     6601 gactgaagtg cggcaacaat atttttatta atgaatcccg catagaccat tgtaacaatc
     6661 tttagcgttt cagcatcagt gatgcggcgg ccatccacca gttgctgttc gatacccaat
     6721 ttctcagcca tcttggtgg
//
LOCUS       DAORLS010000166         6690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6178_length_6690_cov_1.943783, whole genome shotgun sequence.
ACCESSION   DAORLS010000166 DAORLS010000000
VERSION     DAORLS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_6690_cov_1.943783"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="PK977_12340"
     CDS             complement(<1..1465)
                     /locus_tag="PK977_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRF18954.1"
                     /translation="MPDNQYDAIVIGSGISGGWAAKELTEKGLKVIMLERGRNIEHIK
                     GYVNAMKESWEFPHRGSRTQQMIKDYPVLKRDYPLNETVLDYWCKDSEHPYTEVKRFD
                     WYRGYHVGGRSLMWGRQSYRFNKWDFEANAREGIAVDWPIRYDDLAPWYSYVERFAGI
                     QGSKEGLEVLPDGEFMPAMDLNCVEKEVKKRMEAHYKGSRHLIIGRSANITQPHYART
                     NCQYRNRCWRGCPFGGYFSTQSSTLPAAMNTGKLTLRPFSIVTKILYDKDKKKATGVE
                     VLDAETNKTYEYKAKIVFVCASALNSTWILMNSATDIWPDGLGSSSGELGHNVMDHHF
                     RCGASGTVEGFSDKYVYGRRPTGIYIPRFRNIYNDKRDYLRGFGSQGGAGRGRGVEVA
                     ELTIGADLKEALSVPSDNWGFGITAFGEMLPYHENKVTLDKDKKDKWGLPVLSFDVEI
                     KENEKKMRIDMINDAKEMLEAAGVKNVKANDWGYHVGM"
     gene            complement(1509..>1738)
                     /locus_tag="PK977_12345"
     CDS             complement(1509..>1738)
                     /locus_tag="PK977_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HRF18955.1"
                     /translation="RTIKDNNLYFGHYHTAGVPGRHEIDESQELFYPAIMRAIAATGY
                     TGYVAQEFIPSAKENKDKVAALKKAIRICDI"
     assembly_gap    1739..1799
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1800..2411)
                     /locus_tag="PK977_12350"
     CDS             complement(<1800..2411)
                     /locus_tag="PK977_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HRF18956.1"
                     /translation="MASNKKSRRDMLKQLAAGSAAVIAMPAMSSFDTLNEDLKLKGNI
                     NHSVCAWTFSSLSLEELCLTVKKIGFSAIDLLTPKDWPTVQKHGISCSMCYTAGKISL
                     TEGWNRKENHSWLIKDFEEAIPLVSKAGYKNLICFSGNRKGMDDETGLKNCTEGLKQV
                     IALAEKQNVIIQMELFNSKVDHKDYMADKSTWGVELCKRIGSPN"
     gene            complement(2422..3627)
                     /locus_tag="PK977_12355"
     CDS             complement(2422..3627)
                     /locus_tag="PK977_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HRF18957.1"
                     /translation="MKLTTRFQLSAMMFMNFFIWGIWFVTMGTYLGSKLAADGVQIGL
                     AYGTQSLGAIIAPFIIGLIADRYFSAQKILGVLHIAGAGLMYCLSTIDSFSSFYPVLL
                     VYMVIYMPTLALVNAISFKQMHNPEKEFSFIRVWGTIGWIVAGLLIGWLALEKKGSLQ
                     NTFLISAIVSAALGLYSFSLPDTPPPKAGTKASFGEILGLDALGLLKDRNFLVFFLAS
                     MLICIPLAFYYQETNIFLNEAGVENAAGKMTMGQMSETLFLFLMPLFFTRLGVKKMLL
                     IGMLAWVIRYLCFGFGDAGSGGWLLYAGIILHGICYDFFFVTGQIYTDQRAGEKIRSS
                     AQGMITLATYGVGMLIGFWFAGYIAEYFKTAEGHNWKNIWMVPAAIAGVIMLLFLLLF
                     RDPKNNQSK"
     gene            3767..4927
                     /locus_tag="PK977_12360"
     CDS             3767..4927
                     /locus_tag="PK977_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF18958.1"
                     /translation="MQNWKLRMGMIGGGPGAFIGAVHRIAAGMDGEIELVCGAFSSDP
                     EKSKQAGEMLYLNSSRVYGSYREMIKREAELPAAEKMDFVSIVTPNYLHFEPAKLAIE
                     NGFHVVLDKPMTFDLSEALQLKELVAKKGAIFCLTHTYTGYPMVKEARQQIASGKFGK
                     IRKVYVEYPQGWLSTYLEGTEQKQASWRTDPAKSGIAGAMGDIGTHAFNLAEYITGLK
                     VVKICADVNSVVDGRKLDDDGAVLLKFENGASGVLLATQVAAGEENNIKIRVFGEKGG
                     IEWKQEDANSLIVKWLDKPTEIWRTGTGYVSSFASHNTRVPAGHPEGYLEAFANHYRN
                     FALCVKARMNATAAKQEWKDFPGIEEGVRGMLFIEKVIESGRSNKKWIDFNQ"
     assembly_gap    4966..5004
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5005..>5658
                     /locus_tag="PK977_12365"
     CDS             <5005..>5658
                     /locus_tag="PK977_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRF18959.1"
                     /translation="NAPFNNLRSICLWAEALGYKAVQIPSWDSRCIDLKKAAESKTYA
                     DEIKGIVNECGLEISELSTHLQGQLVAVHPAYDTMFDGFAPENVRGNAKARTDWAIQQ
                     VKYGAAASKNLGLAAHPTFSGALLWHTFHPWPQRPAGLVEEGFKELAKRWLPILNYFD
                     ECGVDVCYEIHPGEDLFDGITYEMFLKEVNNHPRACLLYDPSHFVLQQLDYIQYIDFY
                     "
     assembly_gap    5659..5668
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5669..6019
                     /locus_tag="PK977_12370"
     CDS             <5669..6019
                     /locus_tag="PK977_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRF18960.1"
                     /translation="HERIKAFHVKDAEFNPSGKQGTFGGYQGWAERAGRYRSPGDGQV
                     DFKTIFSKLTQYNYSGWAVMEWECCIKHPEDGAREGAAFINNHIIRVTDKAFDDFAAT
                     AKNDKLNRNILGLD"
     gene            6075..6690
                     /locus_tag="PK977_12375"
                     /pseudo
     CDS             6075..6690
                     /locus_tag="PK977_12375"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
BASE COUNT         1985 a         1346 c         1367 g         1882 t
ORIGIN      
        1 ccatccctac atgataaccc cagtcattgg cctttacatt tttaaccccg gcagcttcca
       61 gcatttcttt ggcatcattt atcatgtcta tcctcatttt cttttcattc tcttttattt
      121 caacgtcaaa agatagaaca ggcaagcccc acttatcttt tttgtctttg tccagagtta
      181 ccttgttttc atgataaggc aacatttcac caaaagccgt aattccaaaa ccccaattat
      241 ccgaaggaac gctaagggct tctttcaggt cagcaccaat tgtaagttct gctacttcta
      301 caccgcggcc acgaccggca ccaccctggg aaccaaatcc cctcaggtaa tctcttttat
      361 cgttataaat attacggaaa cggggaatat aaataccagt aggccttcgt ccataaacat
      421 acttgtcgct aaatccctca acagttccgc tggctccaca acgaaaatga tgatccatta
      481 cattatgccc taattcacca ctgctgctgc ccaatccgtc tggccatata tctgttgcag
      541 aattcatcaa tatccatgtg gaatttaaag cagatgcgca tacaaataca atctttgcct
      601 tgtactcata cgttttattg gtctcagcat ccagtacttc tactccggta gctttctttt
      661 tgtctttatc gtaaagaatt tttgttacga ttgagaaagg tcttagtgta agtttacctg
      721 tattcatcgc agccggcagg gtagaagact gcgtactgaa ataacctcca aacgggcatc
      781 cccgccagca acggttccgg tactgacagt tcgttcttgc atagtgtggt tgagtaatat
      841 tagcacttcg gccaatgatc aggtgacggc ttcccttgta atgagcttcc attcgtttct
      901 ttacttcttt ctctacgcaa ttcagatcca tagcaggcat aaattcacca tccggcaata
      961 cttctaatcc ttctttgctt ccctggatac cggcgaatcg ttctacataa ctgtaccagg
     1021 gagcaaggtc atcatacctg attggccagt caactgcaat accctccctg gcattggctt
     1081 caaaatccca tttattaaaa cgataactct ggcgacccca catcagagaa cgtcctccca
     1141 catgataacc acgataccag tcaaaccgct tgacttcagt gtaaggatgc tctgaatcct
     1201 tgcaccaata atctaaaaca gtttcgttta acggatagtc tctttttaaa acagggtaat
     1261 ctttgatcat ttgttgtgta cggctacccc ggtgtggaaa ctcccatgat tctttcatgg
     1321 catttacgta acctttgata tgctctatat ttcttcctct ttccagcatg atcactttaa
     1381 ggcctttttc agtcaattct ttggcagccc atccgccact gataccggaa ccaattacaa
     1441 ttgcatcgta ctgattatct ggcataatat atttcctgat ttaatattta atactgagta
     1501 tcactttttt atatatcaca aattctaatt gcttttttta aagctgcaac cttgtctttg
     1561 ttttccttag ctgaaggaat aaattcctgt gctacataac ccgtgtatcc tgttgctgca
     1621 atggcccgca taatggcagg gtagaaaagc tcctgtgact catctatttc atgtcttccc
     1681 ggtacaccag cagtgtgata atgtccaaaa taaaggttgt tatcttttat tgtccttann
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1801 ttcggcgaac cgattctctt gcataattct acgccccagg tacttttatc agccatataa
     1861 tctttgtgat caactttgct gttaaataat tccatttgaa taatgacatt ttgcttttct
     1921 gccagtgcta ttacctgttt taacccctct gtacaatttt tcaatcctgt ttcatcatcc
     1981 atgcctttac ggttaccgct aaagcaaatc aggtttttat agcctgcctt gcttacaagc
     2041 ggaatagctt cttcaaaatc ctttatcagc caggagtggt tttcctttcg gttccatcct
     2101 tccgttaagc tgatctttcc ggcagtataa cacatggagc aactaattcc atgtttctgc
     2161 acagttggcc aatctttggg agtaagcaaa tcaatagcag aaaagccgat tttcttcacg
     2221 gtcaaacata attcttctaa ggaaagtgaa ctaaaggtcc atgcacaaac agaatggttg
     2281 atattgccct tcagttttaa atcttcgttt agggtatcga aagaactcat agcaggcatt
     2341 gcaataacag ctgctgaacc agccgctaat tgtttcaaca tatcccgtct tgatttctta
     2401 tttgaagcca tttttcacaa attactttga ttgattgttt ttgggatccc tgaaaagcaa
     2461 caagaacagg agcatgatta caccagcgat tgccgcaggc accatccata tatttttcca
     2521 gttatggcct tcagctgttt taaaatattc agctatataa cctgcaaacc agaagccaat
     2581 caacattccc acaccatatg tagccaatgt gatcattcct tgtgcagaag aacgtatctt
     2641 ttcacctgcc cgctggtcgg tgtaaatctg tccggtgaca aagaagaaat cgtagcagat
     2701 cccatgtaaa ataatccctg catacaaaag ccatcctccg ctgccggcat caccaaatcc
     2761 aaaacacaga taacggatta cccaggccag cattcctatc agcagcatct tttttactcc
     2821 cagccttgtg aagaataatg gcatcaggaa caagaacagc gtttcgctca tttgtcccat
     2881 tgtcatttta cctgccgcat tttcaacacc agcttcattc agaaaaatat ttgtttcctg
     2941 gtagtagaaa gccagcggaa tacaaataag cattgaagca agaaagaata caagaaagtt
     3001 cctgtctttc aataatccaa gggcatcaag ccctaaaatt tctccgaaac ttgcttttgt
     3061 acctgctttg ggaggcgggg tgtcaggtaa agaaaaacta tatagaccga gtgctgctga
     3121 aacaatcgct gatatcagaa aagtattttg aagtgagccc tttttttcta atgccagcca
     3181 gccgatcaat aaacctgcca caatccatcc aatagtaccc caaacacgga taaaagagaa
     3241 ctccttttcc gggttgtgca tctgcttgaa ggagatggca tttaccaatg ccagtgtagg
     3301 catgtatatt accatataga ccaataacac aggatagaag gatgaaaaac tatcaattgt
     3361 tgacaggcaa tacatcaacc cggcaccggc tatatgcagt actcctaata ttttttgagc
     3421 agaaaaataa cggtcagcta tgagtccgat tataaaaggc gcaataatgg ctcccagtga
     3481 ttgtgttcca taagctaatc caatctgtac accatctgct gccaatttag aacctaggta
     3541 agttcccatg gttacgaacc agatgcccca gataaaaaaa ttcatgaaca tcatggcgga
     3601 taattggaag cgggtagtca gtttcataca caagctgttg attgattcgt tagttttgaa
     3661 aaatacgttg aaaaactaaa tgtactattt ttaaatgcac atttttgtgg tatagataat
     3721 tttgcggaaa tagtattaat gttgttagta atatcaaacg tttgcaatgc aaaactggaa
     3781 attaaggatg ggaatgatag gtggagggcc gggtgctttt ataggtgcag tgcatcgtat
     3841 tgccgccggt atggatggcg aaattgaatt ggtatgcggg gcttttagca gtgatccgga
     3901 aaaatcaaaa caagcaggag agatgctgta tctgaatagc agcagggttt acggttcata
     3961 tagagaaatg ataaaaaggg aagcagaact tccggctgct gaaaaaatgg attttgtcag
     4021 catagtaacc cccaattacc ttcattttga acctgcaaag cttgcaatag aaaatgggtt
     4081 tcatgtggtg ctggataaac ctatgacctt tgacctctca gaagcgttac aactaaaaga
     4141 actggttgcc aaaaaaggag cgatattttg tcttacacat acttacacag gttacccgat
     4201 ggtaaaagag gcaagacaac aaatagccag tggtaaattt ggcaaaatcc gaaaagtata
     4261 tgtggaatac ccgcaaggat ggctcagcac atatcttgaa ggaacagagc aaaagcaagc
     4321 ttcgtggcgc acagatcctg ctaaaagtgg tattgcaggt gctatgggtg atataggtac
     4381 gcatgctttc aatcttgccg aatatataac cgggctgaaa gtggtaaaga tttgcgctga
     4441 tgtgaactca gtcgtagacg gaagaaagct cgatgatgac ggagctgttt tactgaagtt
     4501 tgaaaatggc gcaagcggag tattgttggc tacacaggtg gctgccggtg aagaaaataa
     4561 tatcaagatc agagtatttg gagaaaaggg tggtatagaa tggaaacagg aagatgcaaa
     4621 tagccttata gtaaaatggc tggataaacc aactgagata tggaggaccg gtacagggta
     4681 tgtcagcagc tttgcttcac ataatacaag agtgcctgct ggtcatccgg aaggatattt
     4741 agaggcattt gctaatcact atcgaaactt tgcattatgt gtaaaggccc ggatgaacgc
     4801 cactgccgca aaacaggaat ggaaagattt tcccggcatt gaagaaggag tcaggggaat
     4861 gttattcatt gaaaaagtga ttgaaagtgg cagaagtaat aaaaaatgga ttgattttaa
     4921 ccagtaacca atagcatgac aacaataaaa ggacctggaa tatttnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnaacgcc cctttcaata atcttcgttc aatttgttta
     5041 tgggcagaag ctttaggata caaggcagtg caaattcctt catgggattc ccgttgcatt
     5101 gatttgaaaa aagctgctga aagtaaaacg tatgcagatg aaattaaagg gatagtaaat
     5161 gaatgcggtt tagaaatatc tgagctgtca acacatttgc aaggtcagct ggtagcagtg
     5221 catccggctt atgacaccat gtttgacggt tttgcacccg aaaatgtcag gggaaatgca
     5281 aaagcaagaa ctgattgggc catacagcag gtgaaatatg gagccgcagc ttcaaagaac
     5341 ctgggcttag cagcccaccc cactttcagt ggtgctttgt tgtggcacac ttttcatccc
     5401 tggccgcaac ggcctgcggg attggtggaa gaaggtttca aagaattggc aaaaaggtgg
     5461 ctccctattc tgaattattt tgatgagtgt ggtgtggatg tctgctacga aatacatccg
     5521 ggtgaggatc tctttgatgg tattacttat gaaatgtttt taaaagaggt aaataatcat
     5581 ccaagggcat gtttgcttta tgatccttca cattttgtac tgcagcaact ggattatatt
     5641 cagtacatcg atttttatnn nnnnnnnnca tgaacgtata aaagcttttc atgtaaagga
     5701 tgcggagttc aacccaagtg gcaagcaagg tacatttgga ggataccagg gttgggctga
     5761 aagggcaggc cgctatcgtt ctcccggtga cgggcaagtg gatttcaaaa caattttcag
     5821 taaactaacg cagtataatt attccggctg ggcagtaatg gagtgggagt gttgtattaa
     5881 acacccggaa gacggtgcca gagagggtgc agcctttatt aataatcata ttatccgggt
     5941 tacagataag gcttttgatg attttgcagc tactgcaaag aatgataaac ttaacaggaa
     6001 tattttagga cttgattaat taaaagtaaa tgaataaaag gattcagttt tcggtgctgg
     6061 taacaataac ggttatgctt ttagcctgct ctggttcaaa attttctacc ggcagcgaaa
     6121 atattttaac cggcgatgaa aagaaagagg ggtggcaatt actctttgat gggaaaacaa
     6181 caaaaggctg gcacaaatat ggcgggcaaa ctgttggttc agcatggaaa gttagagatg
     6241 gttatttata tctcgatact tcggaaaaag aaaactggca gataaagaat ggaggagata
     6301 ttgtttcaga tgatgaattt gaaaactttc acctcaaact ggaaaattgc aaaaaatggt
     6361 aacagcggga taatctttta cattcatgag gataaagcaa agtataactg gccgtgggaa
     6421 acaggcccgg aaatgcaggt acttgataat aatggccacc cggatgcaaa aattattaag
     6481 catcgttccg gagatcttta tgatctgata tcctgttcaa aagaaacaac caagccctat
     6541 ggtgaatgga acttagcaga agtaaaatgt gtaaatggca ggcttgatct ctatctcaat
     6601 ggtgaaaatg ttgtgacaac aaagctgtgg gatgacaatt ggcgaaaaat ggttgccggc
     6661 agtaagttca aaaacatgcc tggctttggt
//
LOCUS       DAORLS010000167         6682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6192_length_6682_cov_1.910216, whole genome shotgun sequence.
ACCESSION   DAORLS010000167 DAORLS010000000
VERSION     DAORLS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6192_length_6682_cov_1.910216"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    314..386
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<387..613)
                     /locus_tag="PK977_12380"
     CDS             complement(<387..613)
                     /locus_tag="PK977_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRF18961.1"
                     /translation="MGNRTFTMIKPDAFKNGHAGAILDRILQEGYNIRALKMTKLTPE
                     KAAEFYAIHKERPFFKELVEFMCQGPIIAAI"
     assembly_gap    725..778
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            792..>1078
                     /locus_tag="PK977_12385"
     CDS             792..>1078
                     /locus_tag="PK977_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRF18962.1"
                     /translation="MLTEPKKVVITTHQKPDADAMGSSLALRLLLLDLGHSVTVISPT
                     NWAGWVDWMPGVKEVLDYEMNRQKANAILDEAEWLFCLDFNIFRRTKTMTE"
     assembly_gap    1079..1119
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1120..1795
                     /locus_tag="PK977_12390"
     CDS             <1120..1795
                     /locus_tag="PK977_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRF18963.1"
                     /translation="TKILIDHHQEPDVASFDYGKSDVSKSSTCEMIYDFITESGYGDK
                     INEEISECLYAGIVGDTGSFRFSSAHQGVHKLVADLKSKGLNHGPVHDRLFDNFLENR
                     LRFVGHVLLHRMEVFYEYNTVLIAIPKSDLIKYQIKTGDTEGLVNFPQSIQGIKLVAL
                     VIDRDEERKWSFRSKGDFDCNTFARKYFEGGGHYNAAGGRSSDTLEMTVKRFMEALKE
                     NEHILQ"
     gene            1905..2759
                     /locus_tag="PK977_12395"
     CDS             1905..2759
                     /locus_tag="PK977_12395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF18964.1"
                     /translation="MPYKIYRSGDTQRIVHGNFVKVSFTRTIKDSVAFTTDGGIPAYI
                     PITQYLEPYDISELWTKLNLGDSLVTIQMVDTFLKRSPMGVPPSLKKGDRIVTTVKIL
                     KIFQTDSAAKADEQKENEAFVTKEINFLDKYLKEKGITAEKTPSGAWVQIINPGNGNP
                     IDSGKFVSVDYTGTSFSGKKFDSNTDPAFNHVQPLQFVVGSGQMIKGFDEGVKFLKPG
                     GEAKVYIPSMLAYGPSPDPRSGIKPYEHLIFDIKVTKVDDKAPTRAEMDELRKQQEQK
                     VDTTQGKK"
     assembly_gap    2795..2804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3022..>3696)
                     /gene="folP"
                     /locus_tag="PK977_12400"
     CDS             complement(3022..>3696)
                     /gene="folP"
                     /locus_tag="PK977_12400"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRF18965.1"
                     /translation="ASILDIGGQSSRPGSKPVSADEEITRVIRPIEAITKRFPESYIS
                     VDTYYSIVAKEAVDAGAVIINDISAGKLDDLMIPVVSSLQVPYVLMHMKGTPQTMQEK
                     LHYENISREILDFFINKVQEIREAGITDIIIDPGFGFGKAIGHNFQLLKNFSLFKMLG
                     FPLLAGLSRKSTIYKTLKISPEDSMNGTTVLNTISLLNGASILRVHDVKEAAEAIKLF
                     DAYQKA"
     assembly_gap    3697..3812
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3813..4479
                     /locus_tag="PK977_12405"
     CDS             <3813..4479
                     /locus_tag="PK977_12405"
                     /inference="COORDINATES: protein motif:HMM:NF019282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRF18966.1"
                     /translation="GFSSNNNLPLQFRVNISLSSLFLMLRYIDRNKSHQAINYRNFIF
                     IMTTTNQQYDQVVKDCKEIFLKKAKDYGTAWRVLRTISIVDQIFIKAQRIRTIQEKGE
                     QKVSDGIATEFKGIINYAVIGLIQLQLPSDAPEEMNIDEVSALYDKHIITARSLMQDK
                     NHDYGEAWRSMSQESFTDLILMKLQRIRQILSNDGKTIISEGIDANYHDIINYAVFAL
                     ILM"
     gene            4481..5674
                     /locus_tag="PK977_12410"
     CDS             4481..5674
                     /locus_tag="PK977_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRF18967.1"
                     /translation="MKGTITFFRIITGFLFIFSGLVKANDPHGLSYKMQEFFEVWGIH
                     GFNEWTLLMSVLMNAFEIIAGFALLLGWRIKLFSWLLLLLIIFFTFLTGYTYVTGKPT
                     NCGCFGDCLPITSKTSFLKDVVLTIMIGFLFWKRNEIKPLLGNRVGTGFMLAITIFSF
                     GFQWYTLTYLPVVDCLPFKKGNNIPEKMKMPANAVPDSTVVTFVYRKEGNEVEFTADK
                     FPADFSKDKYEFIRRYDKVVRKGKNNEPPIKGFALSGDSNIDSAQYVLEQPYAVLLFI
                     EKADEAGSGWKKKFEKIYTVAKSKNIPAYIITAASGDKNKLTDGTAFNDITVYKCDYK
                     AIQTAARSNPCIYLLKKGTVLNKYSGQSMGMIKDELNKLPVQPPPVTVPAPEPTVIDS
                     TIIQN"
     gene            5680..>6682
                     /locus_tag="PK977_12415"
     CDS             5680..>6682
                     /locus_tag="PK977_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF18968.1"
                     /translation="MLQFIIKKTLYGIGVLLGVVMLVFFLFQGFGDPSRLVMGQSGDS
                     TTQANIRKELYLDQPKWKQFVFYLNDVSPVCLYSKKEITSKKLRGFFIGGENKFGIKI
                     PYLRKSYQSKREVWTILMQALPGTLMLAIAAMFIAMLIGIPLGVMAAVKQNTWLDTSA
                     IFSSIVGISAPSFFMGIIMVYLFGFVLSDLTGLQIAGSWFVIDEEGNRRLTLQNLLLP
                     AFTLGIRPLAIITQLTRSAMLDVLDQDYIRTAYAKGLGKRTVIWKHGLRNALNPVITA
                     ITGWFAELLAGAFFVEYIFGWQGIGKVTVDALEKLDYPVVMGSVLISATFFILINILA
                     "
BASE COUNT         1967 a         1185 c         1369 g         1867 t
ORIGIN      
        1 aacgggctgg gtataccaaa agaaggttat ggcttctcca ataacgttac aggtacattc
       61 gggcctcctt ctttcaagag agccagtttt aagtataacg gggatttggt ttccgtggta
      121 ataagaacca ggtattaaaa acaaaaccag ctcgaggaac tggctttgtt tgtatttaat
      181 aatgcttaga attgttctaa aactgagaac ataaaattcc cttcaattac tgcattctca
      241 tcactgtcgc ttccgtgcac tgcattttca cctactgaag cggcgtataa tttacggata
      301 gtgccttctt cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa tcgcagcaat aattggcccc tggcacatga
      421 actcaaccaa ctccttaaag aaaggccttt ctttatgaat tgcataaaac tctgcagcct
      481 tttcaggggt aagcttagtc atttttaatg ccctgatatt gtatccttcc tgtaaaatcc
      541 tgtcaagaat agcaccggca tgcccgtttt taaaagcatc cggtttgatc attgtaaatg
      601 tacgattgcc cataatttgt ttttttcggc cgcaaaggta acggaaactt ggtcagtatt
      661 taaagactaa ttgttaatta gtaaagaaag cgattacgga aagcttcaat tacattgaat
      721 accannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
      781 aattattccc tctgctgact gaacctaaaa aagtggtgat aactacccac cagaagccgg
      841 atgctgatgc aatgggttct tcgctggcct tgcgccttct cctgcttgat ttgggtcact
      901 ctgttactgt tatttctccc accaactggg ccggctgggt cgattggatg ccgggtgtga
      961 aggaagtcct ggactacgaa atgaacaggc aaaaagcaaa tgccattttg gatgaggctg
     1021 aatggttatt ctgtcttgat ttcaacattt tccgccgcac aaaaacgatg acagaaaann
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt acgaaaatcc ttattgacca
     1141 tcaccaggaa cccgatgtag catcatttga ttatgggaaa agtgacgtat caaagagttc
     1201 tacctgtgaa atgatctatg attttattac tgaatcaggc tatggagata agattaatga
     1261 ggaaataagt gagtgcttgt atgcgggtat agttggggat acaggctctt ttcgcttctc
     1321 atcagctcac cagggagtac ataaactggt ggcagatctt aaatcgaagg gtttaaatca
     1381 tggcccggtt catgaccggt tgtttgataa ttttttagaa aaccgtcttc gttttgttgg
     1441 tcatgtgctt ttgcacagaa tggaagtatt ctatgaatac aataccgtac tgatagcaat
     1501 tcccaaaagc gaccttatca aataccagat taaaacagga gatactgaag gattggtcaa
     1561 ctttccacaa agtatccagg gcataaagtt ggtggcactt gttattgaca gggatgaaga
     1621 aagaaaatgg agcttccgga gcaagggaga ttttgactgt aatacttttg caagaaaata
     1681 ttttgaaggt ggggggcatt ataatgcagc cggtggccgc agcagtgata cacttgaaat
     1741 gactgtaaag cgttttatgg aagcattaaa agaaaacgaa catattttac aataaaagga
     1801 taaatacaaa taaaaatatg aagactggaa tttcaattgt tgttttaata accgtattaa
     1861 ttgcggggtg tggaagaacc aattaccaga aaactcccgg aggcatgccc tacaagattt
     1921 atagaagcgg ggatacccag cgcattgttc atggtaattt tgtgaaagta agttttacca
     1981 gaacaataaa ggatagtgtg gcatttacta ccgacggtgg tattcctgcc tacataccta
     2041 ttacacaata tttggagccc tatgatatat ctgaactgtg gactaaactc aatctcggtg
     2101 acagtcttgt aactatccag atggttgata cttttttgaa aagatcgcca atgggggtac
     2161 ctccgagtct taagaaaggt gacaggatag ttaccacagt taaaatcctg aagatattcc
     2221 agacagattc tgctgctaaa gcagatgaac aaaaagaaaa tgaagcattt gttactaaag
     2281 aaatcaattt ccttgataaa taccttaaag aaaaaggcat aacggctgaa aagactcctt
     2341 ctggggcatg ggtacagatc attaatccag gcaacggaaa tccaattgat tcaggcaagt
     2401 ttgtatccgt agattataca ggcaccagtt tctccggaaa aaaattcgac agtaataccg
     2461 atccggcatt caaccatgtg cagccacttc aatttgtagt tggctcggga caaatgatca
     2521 aggggtttga tgaaggagta aagtttttga agcccggagg cgaagcaaag gtttatatac
     2581 cttcaatgct ggcctatggt ccaagtcccg acccccgttc aggtattaag ccttatgagc
     2641 acctgatctt cgatataaaa gttacaaaag tggatgacaa agctccgaca agggcagaaa
     2701 tggatgaatt aagaaagcaa caggagcaaa aagttgatac gactcaagga aaaaaatgat
     2761 gcaaataatg agataatttc tcaacttcgt attcnnnnnn nnnnaataga tgggtaattt
     2821 gagtggcccc gtttttagtc caggatctga ttaaacccgt ccgcaccctg aaaacaaaat
     2881 accctagaaa tcataccgta ctttttgctt tgcctcatat tagtccgtag aattatgaaa
     2941 cgttcaatgc aattattagg ttaaggagat gattgcaatg tcttgcaaag aagctttgcc
     3001 cgagggcaag gcttcttcgt ttcatgcctt ctggtatgca tcgaatagct taatggcttc
     3061 cgccgcttct tttacatcat gcactctcag aatacttgcc ccgtttagca aagaaatcgt
     3121 attgagtaca gttgtgccat tcatagagtc ttcaggagaa atcttgagtg ttttgtatat
     3181 agtggacttt ctggaaaggc cggccaacaa aggaaagccg agcattttaa aaagggaaaa
     3241 gtttttgagc aactggaaat tgtgaccgat agctttgcca aatccaaaac ccgggtcaat
     3301 gattatatca gtaatgccgg cttccctgat ttcttgtact ttgttaataa aaaagtcgag
     3361 tatttcccgg ctaatatttt catagtgcag tttttcttgc atcgtttgag gagtaccttt
     3421 catatgcatc agtacatagg gaacctgtaa ggatgaaacg acaggtatca tcaggtcatc
     3481 tagttttccg gcgctgatat catttatgat tactgcaccg gcatctaccg cttcctttgc
     3541 aacaattgaa taataggtat caacagaaat atacgattca ggaaatctct ttgtgatagc
     3601 ttcaatgggt ctgatcactc tagttatttc ttcatccgca gataccggct tactgcccgg
     3661 cctgctgctt tgtcccccga tgtctaaaat actggcnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaggttttt ccagcaacaa taatcttcct
     3841 ttacaattca gggtaaacat tagccttagt tcgttatttt tgatgctaag atacattgac
     3901 cgcaataaaa gccatcaggc aataaattac aggaatttca tcttcattat gacgacgacc
     3961 aaccagcaat atgaccaggt agtaaaagac tgcaaagaga tttttttaaa aaaagctaaa
     4021 gactatggaa ctgcatggcg ggtactcaga acaatttcta tcgttgacca gatctttatc
     4081 aaagcacaac gaatccgcac catccaggaa aaaggagagc agaaagtttc tgatggtatt
     4141 gctactgaat ttaaaggcat cattaattac gctgtgatag gcttaattca gctgcagtta
     4201 ccgtcagatg caccggaaga aatgaatatt gatgaagtat ctgctttata cgacaagcat
     4261 attatcactg ccaggtcatt gatgcaggat aagaaccatg actatggcga agcatggaga
     4321 agcatgagcc aggaaagctt caccgatctt atcctgatga agttgcagcg tatccggcaa
     4381 attctgagca atgacgggaa aactatcatt agcgaaggaa tcgatgccaa ttaccatgac
     4441 atcattaact atgcggtttt tgcattaata ttaatgtgat atgaagggaa caattacttt
     4501 cttccgaatt attaccggct ttttattcat cttttccgga ctggtaaaag ccaatgatcc
     4561 gcatggtctc agctataaaa tgcaggagtt ctttgaagta tggggtatcc atggttttaa
     4621 tgaatggaca ttattaatgt ctgttcttat gaatgcattt gaaatcatag cggggtttgc
     4681 tttgttattg ggctggagga ttaaactttt cagctggtta ttattactat tgatcatctt
     4741 ttttactttt cttacggggt atacgtatgt gacaggtaag ccaacgaatt gcggatgttt
     4801 tggtgattgt cttccgatta cttcaaaaac atcttttttg aaagatgtag tgcttaccat
     4861 catgattggc ttcctattct ggaaacgaaa tgaaataaaa cctctcctgg gaaatagggt
     4921 gggtaccggt ttcatgctgg caataaccat ctttagtttt gggttccaat ggtacacatt
     4981 aacttattta cccgtggtgg attgcctgcc ctttaaaaaa ggaaataaca ttcccgaaaa
     5041 gatgaaaatg ccggccaatg cggtacccga cagcactgta gtaacttttg tgtacaggaa
     5101 ggaaggaaac gaggttgaat tcactgcaga taaattcccc gctgatttta gtaaagataa
     5161 atacgaattc atcagacgtt atgataaggt agtaagaaaa ggaaaaaata acgaaccccc
     5221 gataaaagga tttgctttat ccggtgattc taatatcgac tccgcccaat atgtattaga
     5281 acagccttat gctgtgttgt tatttattga aaaagcagat gaggctggtt ccggctggaa
     5341 aaagaaattt gaaaaaatat atactgtagc gaagagtaaa aatatcccgg catatataat
     5401 tacagcagct tcaggagata aaaataaatt gactgatgga acggcattta atgatattac
     5461 agtttataaa tgtgattata aagctatcca gacagctgcc agaagcaatc cctgcattta
     5521 tttattaaaa aaagggacgg tactgaataa atacagcggg caaagtatgg gtatgattaa
     5581 agatgaactc aataaactgc ctgtacagcc gccaccggta actgtcccag cgcctgagcc
     5641 aactgtcatt gattcaacta ttattcaaaa ctgacagaga tgctgcaatt catcatcaaa
     5701 aaaacactgt atggtatagg agtgctttta ggtgtggtga tgcttgtgtt cttcctcttc
     5761 cagggctttg gtgatccgtc acgattagta atgggacaat cgggtgattc aactactcag
     5821 gcgaatatcc gtaaagaact atatcttgat caaccaaagt ggaagcaatt tgttttttat
     5881 ctcaatgatg tttcgcctgt ttgtctctac agcaaaaaag agataacatc taaaaaacta
     5941 aggggttttt ttataggagg tgaaaataag tttggtataa aaattcctta cctgcgaaag
     6001 tcctaccaaa gtaaaagaga ggtttggacc atactaatgc aggcacttcc ggggacactg
     6061 atgctggcca tagcagctat gtttattgct atgctcatcg gaattccttt gggtgtaatg
     6121 gcagcagtaa aacaaaatac atggttagat acctcagcga tattttcaag cattgtgggt
     6181 atttccgcac catcattttt tatgggtatt ataatggtat acctctttgg gtttgtatta
     6241 agtgatctta caggtctgca aattgccggc agctggtttg taatagatga agaaggaaat
     6301 agaaggctga ccttgcaaaa ccttcttttg cctgctttca cattgggtat tcgtccgctg
     6361 gcaataatca ctcagttgac ccgaagcgcc atgctggatg tacttgacca ggattatatt
     6421 cgtacagcat atgcaaaagg cctgggcaaa agaacagtga tctggaagca tggattacgt
     6481 aatgcattga acccggttat tacggccatt acaggctggt ttgccgaatt attggcggga
     6541 gctttctttg tagagtatat ttttggttgg cagggtatag gtaaagtcac agtggatgcg
     6601 ttggaaaaat tggattatcc ggtggttatg ggatctgttt tgatttctgc taccttcttc
     6661 attctgataa atatactggc tg
//
LOCUS       DAORLS010000168         6654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6224_length_6654_cov_1.732232, whole genome shotgun sequence.
ACCESSION   DAORLS010000168 DAORLS010000000
VERSION     DAORLS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6224_length_6654_cov_1.732232"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>1351
                     /locus_tag="PK977_12420"
     CDS             20..>1351
                     /locus_tag="PK977_12420"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF18969.1"
                     /translation="MQQATVNLFADMTVQPGSLQPGLVLATASSDNIAPVSVITSPVH
                     NASVLKGIPVTVTGTATDNNTVAGIEISVDGGTTWHVASGTSQWSYTFTPTTTGNITV
                     KCRGFDDSGNMEIAGTVPSSNALQLNVINTTPPEDGPGGPILVISKSSNPFSRYPVEM
                     LRAEGLNEFTAMDISAVNPTVLNNYDVILLGEMPLQASEVSMLSDWVNAGGTLIAMRP
                     DVQLSSLLGITSAGISISDKYLLVNTTAGPGKGIVNQSIQFHGTGDLYNLNGASTLAT
                     FYSDATTPTVYPAVTSNAVGINGGSAVAFLYDLAKSVVLTRQGNPSWSGDERDGTSPI
                     RSNDLFFGNKAGDSQPDWIDLNKVAIPQADEQMRLLTNIIISNNLHRKPLPRFWFLPK
                     GLKAAIVMTGDDHGNNGTTERFNQYVSLSSSNTAEAVADWTAIRGTSYIYPN"
     assembly_gap    1352..1369
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1370..>2642
                     /locus_tag="PK977_12425"
     CDS             <1370..>2642
                     /locus_tag="PK977_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HRF18970.1"
                     /translation="AAAFESLGFEIGLHLNTGCNNWTPPSFQADLTNQSVQFSSAFPG
                     LTAPLTNRTHCIAWSDWSSAAEIQAANGIRLDVNYYYWPGTWVLNRPGMFTGSGLPMR
                     FAKTDGSLIDCYQVATQMTDESGITYSSFCNALLDKAIGPEGYYGVFCANMHTDAGTS
                     AGSDDIIASAQARQIPVISAKQMLNWLDGRNNSSFGSISWNGNQLSFTITNNAGQHNL
                     KAMLPVQGPNNSVLSGITHNGNPVNKTIETIKGIDYAFFDALGGSYTANYINCTSPTA
                     VISSNSPVCYGQPINLTLQSATGQEPFDLVVNCVTYNNVNPGQTFATINTSEISIWGN
                     TGSPTSPSVTDNQPIEIGTKFRSTISGYITGIRFYKGFTNTGTHIGSMWTASGTQLAT
                     ATFTSETASGWQEVRFTNPVAIQPNTTYIASY"
     assembly_gap    2643..2652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2653..>3005
                     /locus_tag="PK977_12430"
     CDS             <2653..>3005
                     /locus_tag="PK977_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HRF18971.1"
                     /translation="GYFAISPGFFSTSGVSNGPITALQAGVDGPNGVFKYGGGFPDGG
                     NTANYWVDVLFSETLTTATSLNYVLTSVTDDNNCNTTAPNISTASVTINPLPVGVLSA
                     QPPFCAGQNVNLVFN"
     assembly_gap    3006..3071
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3072..>3373
                     /locus_tag="PK977_12435"
     CDS             <3072..>3373
                     /locus_tag="PK977_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HRF18972.1"
                     /translation="NSNIQAPATTPVSIWNNSVMGGEPPVNDFDAVELGVKFRSSMSG
                     TISGVRFYKRVQQPNNATYTGRLWKADGTLLATCTFTNITNSGWQEASFSSPVAINA"
     assembly_gap    3374..3465
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3466..4260
                     /locus_tag="PK977_12440"
     CDS             <3466..4260
                     /locus_tag="PK977_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF18973.1"
                     /translation="NGPLTALQSGTDGLNGVFKYGAGGIFPNDSYNNANYWVDVVFNG
                     AGLTQFNLTSITDANLCTSSGNLQTLNVTSSQCSPLPVTMTSLSASAAAKKVTLNWST
                     LTEIDNRGFEIYRSYDAVTWQYIGFIQGAGNSTNRVNYNYDDNNLDPGRYYYRLKQID
                     FNGASKFSNIAAATLYDKGRYFLGQNYPNPFTNTTMIEFALPSRQMVSISLTDVHGRV
                     IKTLLSEVRNAGSHTISFDNQGLSRGIYFYKLEAGGFSDTKKMILQ"
     gene            4354..4842
                     /locus_tag="PK977_12445"
     CDS             4354..4842
                     /locus_tag="PK977_12445"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF18974.1"
                     /translation="MLTIRHTTTADIPLIRELTFKVWPQTYRDILTSEQIDYMLEMMY
                     SPSSLEKQMQDGCSFIIVYDNEQPVGFASYQLTNEEVAKLHKIYVLPSQQGKGTGNFL
                     LNYTIEKVKKLGAVSLQLQVNRNNTAKAFYEKKGFVVIEEADFDIGNGYYMNDYVMEK
                     KL"
     assembly_gap    4897..4906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4907..5312)
                     /locus_tag="PK977_12450"
     CDS             complement(<4907..5312)
                     /locus_tag="PK977_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18975.1"
                     /translation="MKDKVKLFLVSLLFVAPAFVYGQKDSVWLKCPLDEAIIVPPPKN
                     VMKFDEPDLCVGLVSVPDTTVKACITGRVSNVIQNEDEKWDVVFYYRDYYFWYSGLSK
                     VIVKKNDNLKVSQPLGYITPGDKLELSLYKFET"
     gene            complement(5322..5621)
                     /locus_tag="PK977_12455"
     CDS             complement(5322..5621)
                     /locus_tag="PK977_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18976.1"
                     /translation="MYSYDTLSEAVNDLRKRGFIVDFNLQENCLVCHSDKFNIEDFEV
                     AEVYRFEGNTDPADEAIVFGIESKTGVKGVLVSGYGVSAEGMSAEMARKLSLHNR"
     gene            5803..>6654
                     /locus_tag="PK977_12460"
     CDS             5803..>6654
                     /locus_tag="PK977_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRF18977.1"
                     /translation="MSTVSKSIDFSLPYKVADISLAEWGRKEIRLAEAEMPGLMALRQ
                     EYGASQPLKGARVAGCLHMTIQTAVLIETLVALGAEVKWSSCNIFSTQDHAAAAIAAA
                     GIGVFAWKGQTIEEADWCIEQTLFFGGADRPLNMILDDGGDLTNMVLDKYPELVQHVK
                     GISEETTTGVHRLYERVAKGTLPMPAINVNDSVTKSKFDNKYGCKESLVDAIRRATDV
                     MLAGKVAVVGGYGDVGKGSAASLAGAGCRVIVTEIDPICALQAAMDGFEVKKMIDAVK
                     EAYIVVTA"
BASE COUNT         1924 a         1401 c         1329 g         1804 t
ORIGIN      
        1 ttctgctgaa gacatacgaa tgcaacaagc tactgttaat ctctttgcag atatgacggt
       61 tcagccaggc tctctgcaac ccggattagt cttggccact gcctcctcag acaacatagc
      121 ccctgtttca gtcattacaa gtcctgtaca taatgcctcc gtactcaaag gaattcctgt
      181 tactgtaact ggcactgcaa ctgataataa taccgtagca ggcatcgaaa tttcagtaga
      241 tgggggaact acctggcatg tagccagtgg aaccagtcaa tggagttata ctttcacacc
      301 aacaactacc ggaaatatta ctgtaaagtg ccggggcttt gatgatagcg ggaatatgga
      361 aattgcagga acggtcccat catcaaatgc tttacaactt aatgttatca atacaactcc
      421 gccggaagat ggtccgggag gacctatact ggtcattagc aaatcatcca acccctttag
      481 cagatatcca gttgaaatgc tgagagcaga aggccttaat gaattcacag caatggatat
      541 tagtgccgtg aatccaacag ttttaaataa ctatgatgtg attttgttgg gtgaaatgcc
      601 attacaggca tctgaagttt ctatgctctc tgactgggtt aatgccgggg gcacattgat
      661 tgccatgagg ccagatgttc aattatcatc attacttgga ataacttctg caggcatcag
      721 tatttcggat aaatacctgt tggtaaatac tactgctggt cctggaaaag gtatcgtgaa
      781 ccagagcata cagtttcatg gaacgggtga cctgtacaac cttaatgggg ccagcactct
      841 tgcaacattc tattctgatg ctacaactcc tacagtttat ccagcagtca cttcaaatgc
      901 tgttggcatc aacggtggtt cggctgttgc attcctatat gaccttgcca aatcagttgt
      961 actaacaaga caaggcaatc cctcatggtc tggtgacgaa agagacggaa cttcaccaat
     1021 ccgctccaat gatctgttct tcggaaataa agcaggagat tcgcagcctg actggatcga
     1081 tttgaataaa gttgccatac cacaggcaga cgaacaaatg cgattactaa ccaacataat
     1141 tatttcgaac aacctgcatc gtaaacctct gccccgtttc tggttcctcc caaaaggact
     1201 aaaagctgct attgttatga caggagatga tcatggtaat aacggaacaa cagaacgctt
     1261 taatcaatat gtatcattaa gttcttctaa tacagctgaa gcagtagcag actggacagc
     1321 aatcagaggc acctcttaca tttatccgaa cnnnnnnnnn nnnnnnnnng gctgcggctt
     1381 ttgaatcatt aggttttgaa atcggtttac atttaaatac cgggtgcaac aactggactc
     1441 ctccttcttt ccaggctgat cttacaaacc aatcagttca attcagttct gctttcccgg
     1501 gtttaactgc accattaaca aaccgaactc attgcattgc atggagcgac tggtcctctg
     1561 ctgcagaaat acaagccgca aatggtatcc ggcttgatgt taattattat tactggccgg
     1621 gtacatgggt gcttaaccgg cccggaatgt ttactggctc tggcttaccg atgcgatttg
     1681 ccaaaacaga tggttcattg attgactgtt accaagtagc aacacaaatg acagatgaat
     1741 caggtatcac ttatagcagt ttttgtaatg ctttgcttga taaagcaatc ggccctgaag
     1801 gttattatgg tgttttctgt gccaatatgc atacagatgc cggaacatca gccggttctg
     1861 atgacataat tgcatcagcg caggcaagac aaatacccgt aatttctgcc aaacagatgc
     1921 tgaactggct ggatggaaga aacaattctt cctttggttc aatatcttgg aatggtaatc
     1981 agttgtcgtt caccattaca aataatgcag ggcagcataa tcttaaagca atgctacctg
     2041 tgcaaggccc caataatagt gtgttatccg gtattactca caacggaaac ccggttaaca
     2101 aaacaattga gacaataaag ggaattgact atgcattctt cgatgcatta ggcggaagct
     2161 atactgcaaa ttatattaat tgcacatcgc ctactgctgt aatcagttct aattcgccgg
     2221 tttgttatgg acaaccaatt aatctaacgc ttcaatctgc aaccggccag gaaccattcg
     2281 acttagtagt aaattgtgta acttataata atgtcaaccc tggccaaacc tttgcaacaa
     2341 ttaatacttc tgagataagt atatggggca atactggttc accgaccagc cccagtgtaa
     2401 ccgataatca acccattgag atcggaacaa aattccgatc aactataagt ggttacataa
     2461 ctggaataag gttttataaa gggtttacca atacaggtac tcatattggc tctatgtgga
     2521 cagccagcgg aacgcaactt gccactgcaa cctttacctc agaaacagcc agtggatggc
     2581 aagaagtaag gtttaccaat ccggtagcaa ttcagcccaa tactacctac attgcgtctt
     2641 atnnnnnnnn nnaggttatt ttgcaatcag ccccgggttt ttcagtactt caggggtaag
     2701 caacggtccg attactgcat tacaggcagg agttgacggt cctaatggtg tattcaaata
     2761 tggtggtggc ttcccggatg gaggaaatac agctaattat tgggttgatg tgttatttag
     2821 tgagactctg acaacagcta cttcgcttaa ttatgttctt accagtgtaa ctgatgataa
     2881 taactgtaat accacagcac ctaatattag cacagcttct gtaaccatca atccattacc
     2941 agttggtgtt ttatcagctc aacctccatt ctgtgctggt caaaatgtaa accttgtttt
     3001 taacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn naactcaaat atccaggccc cggccaccac accagtaagc atctggaaca
     3121 atagtgtaat gggtggcgaa cctcccgtta atgatttcga tgctgttgaa ctgggcgtta
     3181 aattccgttc ctccatgtct ggcacaataa gcggtgtacg gttttataaa agggtacaac
     3241 aaccaaataa cgctacttat actggccgtc tttggaaagc agatggtaca ctgctggcta
     3301 cttgtacatt taccaatatt acaaacagcg gatggcagga ggccagcttt tccagtccgg
     3361 ttgcaatcaa tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacgg cccactcact
     3481 gctcttcagt cgggaacgga cggtttaaat ggtgtattca aatacggagc cggtggtatt
     3541 ttccccaatg attcatataa taatgccaat tactgggtgg atgtggtgtt caatggcgct
     3601 ggtcttactc aattcaattt aacaagtatt actgacgcaa atctttgcac ctcttcgggt
     3661 aacctgcaaa cattaaacgt tacttcttcc cagtgcagcc ccttaccggt cacaatgaca
     3721 agcctgtcag cctccgctgc agctaaaaaa gtaacattaa attggtccac actgacagag
     3781 attgataaca ggggatttga aatatacaga agttatgatg ctgtcacatg gcaatacatt
     3841 ggttttatac aaggtgcggg aaacagtaca aacagggtta attataacta tgatgacaat
     3901 aacctggatc cgggccgtta ttattaccgg ttaaaacaaa tcgatttcaa cggggcttca
     3961 aaattttcaa atattgcagc agcgactctt tatgataaag gccgatattt ccttggccag
     4021 aactatccaa acccctttac caatactacc atgattgaat ttgcgcttcc gtcaaggcaa
     4081 atggtttcaa taagtctaac ggatgtacat ggccgggtaa taaagacgtt attgtccgag
     4141 gtaagaaatg caggttcaca taccatcagt ttcgataacc aggggcttag cagaggaata
     4201 tacttttata agctggaagc aggtggattc agtgatacaa aaaagatgat tttacaatag
     4261 tcagaattca gcccccaata agatatctaa aaaggttttg ccatggcaaa acctttttag
     4321 tttatatctt ggtcaaaaga atcgataatt accgtgctta caattcgtca tacaactaca
     4381 gcagatatac ctcttatcag ggagctaact tttaaagttt ggccccaaac ttaccgtgac
     4441 atacttacca gcgaacagat cgattacatg ctggagatga tgtacagccc ttcttcactc
     4501 gaaaaacaaa tgcaggacgg atgcagcttt attatcgttt atgataatga gcagcccgtt
     4561 ggttttgctt cctaccaact tacaaatgaa gaggtagcga aacttcataa aatttacgtt
     4621 ttaccctcac agcagggtaa aggaacagga aactttctgc tcaattatac cattgagaag
     4681 gtaaaaaagc tgggagccgt ttcactgcag ttacaggtca acaggaataa cacagcaaaa
     4741 gcattttatg aaaaaaaagg ctttgtagta attgaagaag ccgattttga tatcggtaac
     4801 ggttattaca tgaatgacta tgtaatggaa aagaagttgt gaaccttcgt caatctgtct
     4861 tcatcaccac cttgcaatcc aggaatcttg tgggatnnnn nnnnnngtgt ttcgaattta
     4921 taaagggaca actccagttt atctcccggg gtgatatagc ctaatggctg acttactttc
     4981 aggttatcat tcttttttac gatcaccttt gataaacccg aataccagaa ataataatcc
     5041 ctgtaataaa aaaccacatc ccatttttca tcctcattct gtatcacatt ggaaaccctg
     5101 ccggtaatac aggcttttac agtagtgtcg ggaacactta ccagtccgac acatagatca
     5161 ggttcatcaa acttcatcac attctttgga ggaggaacga tgatcgcttc atctaacggg
     5221 catttcaacc agacactatc tttttgcccg tatacaaatg caggagcaac aaacaacaga
     5281 ctaaccagaa aaagcttaac cttatctttc atacagacaa tttatcggtt gtgcaaactc
     5341 aatttccttg ccatttcagc actcatcccc tcggccgaaa caccgtaacc acttaccagc
     5401 acacctttta cccctgtttt ggactcaata ccaaaaacga tggcttcatc agccgggtca
     5461 gtgttgcctt caaaacggta tacttctgcc acttcaaaat cttcgatatt aaacttatcg
     5521 ctgtgacaga cgaggcaatt ctcctgaagg ttgaaatcaa cgataaaacc tcgtttacga
     5581 agatcattta ccgcctcaga aagagtgtcg taactgtaca tcatttcaac tttatgtaac
     5641 caaatttatt cttatttgtt caaaaccagc gactcatatg gctaatttcc taaacaatat
     5701 cctgctttta gttgtgtata acaagtggca attgaagcag attcacaagg ctttatacgg
     5761 taataaatat tacctttgcg cccatcataa aaagcaatta aaatgtcaac agtatctaaa
     5821 tccattgatt tcagtcttcc ctacaaggtt gctgatatat cccttgctga atggggacgt
     5881 aaagaaattc gccttgccga agcagaaatg cccggtttaa tggctcttcg ccaggaatat
     5941 ggcgcttccc agcccctgaa aggagcccgg gtggcaggtt gcctgcatat gactattcaa
     6001 actgccgtgt tgattgaaac actggtggcc ctgggagctg aagtgaaatg gagttcttgt
     6061 aatattttct ctacccagga tcatgcagct gctgctattg ctgcggctgg tatcggagtg
     6121 tttgcctgga aaggccagac cattgaagaa gctgactggt gtattgaaca aactttgttc
     6181 tttggcggcg ctgaccgtcc cctgaatatg attcttgacg atggtggcga cctgaccaat
     6241 atggttttgg ataaatatcc tgaactggta caacatgtaa aaggtatcag tgaagaaaca
     6301 actaccggtg tacaccgctt gtatgaaaga gtggcaaaag gcactttacc gatgcctgct
     6361 atcaatgtga acgactcagt aactaaatca aagtttgaca ataaatacgg ttgtaaagaa
     6421 tcactggtgg atgctatccg ccgtgccacc gatgtaatgc tggcaggtaa agtagccgtt
     6481 gttggtggtt atggtgatgt gggtaaaggt tctgctgctt cactggcagg tgctggttgc
     6541 cgagttatcg ttactgaaat tgatcctatc tgtgctttac aggcagcgat ggatggtttt
     6601 gaggtaaaga aaatgattga tgcagtaaaa gaagcttata tcgttgtaac agct
//
LOCUS       DAORLS010000169         6642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6237_length_6642_cov_2.046000, whole genome shotgun sequence.
ACCESSION   DAORLS010000169 DAORLS010000000
VERSION     DAORLS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6237_length_6642_cov_2.046000"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK977_12465"
     CDS             <1..213
                     /locus_tag="PK977_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18978.1"
                     /translation="SMQEIANEAALYAKPESPADIAEKMMLLYKDEKLRSQLIEKGRV
                     VVKQYSWEQSAERLWQSIEKAIQQNN"
     gene            238..585
                     /gene="gldC"
                     /locus_tag="PK977_12470"
     CDS             238..585
                     /gene="gldC"
                     /locus_tag="PK977_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HRF18979.1"
                     /translation="MNKSTITIEVATDENKIPDTITWSATDSSAENGQKAKAMMISFW
                     DGAEKAALRIDLWTKDMMVDEMADFFYQTLMTMADTYGRATKYQEDVAEMKDFAKTFY
                     EKFRAKQLKENKA"
     gene            660..1112
                     /locus_tag="PK977_12475"
     CDS             660..1112
                     /locus_tag="PK977_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRF18980.1"
                     /translation="MSLEQKIMTELKAAMLSKDEAALRSLRAVKAAILLAKTSEGGGG
                     ELKEEDEIKLLQKLVKSRKDSLEIYQQQNRPDLAKKEEEEIAVIEKFLPKQLSPEEIK
                     TALASIIAETGASSPADMGKVMGVATKQLAGKADGKTISALVKELLSK"
     gene            1193..>1722
                     /locus_tag="PK977_12480"
     CDS             1193..>1722
                     /locus_tag="PK977_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRF18981.1"
                     /translation="MLLDIVFAVVMVLAIIKGYQRGLVVGLFSLVSVIIGLAAAVKLS
                     AAVAGYIGEAVKVSEEWLPVIAFAVVFIVVVILIRLGANAIEKAVEVVMLGWLNKLAG
                     ILLYVTIYTIVFSVLLFYAEQIKLIQPATIEKSLTYSYIQPWGPKVIDGLGSLIPFFK
                     NMFDELSTFFSGVADN"
     assembly_gap    1723..1740
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1951..2727
                     /locus_tag="PK977_12485"
     CDS             1951..2727
                     /locus_tag="PK977_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF18982.1"
                     /translation="MEFEIKQQDKFKFIEEGNGEPLVLLHGLFGALSNFSSLIQYFRQ
                     HNRVIVPMLPLLEMDILHTSVGGLAKFVHKFLEARDLRNVHLLGNSLGGHVALVHTLK
                     HPERIKSLILTGSSGLFENGMGDSYPKRGDYEYIKRKTELTFYDPNTASKELVDEVYS
                     IVNNRIKAIKIIALAKSAIRNNLGEELNQIKQPTLLVWGNNDTITPPFVAKEFNKLIP
                     NSELYFVDKCGHAPMMEVPDEFNAILHKFLKKLNEPATVA"
     gene            2763..3425
                     /locus_tag="PK977_12490"
     CDS             2763..3425
                     /locus_tag="PK977_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF18983.1"
                     /translation="MLTGELHSQSLPYLHLHDKVYQALQLMNDNHVTHLPIVEGEKYI
                     GLVSEEDLMQADNDNSPLEELQQSFSGISVKTNEHFLKALQIAAENGLSVVPLVNDEN
                     ELTGTVVYNDLLKYASGFMSLNEPGALIVLEIPSNQYSFNEISKLVETNDAQITQLNT
                     SNDIESGMMQVTIRINKPEVSDIIATFQRYDYNVKYFFGEELYTNELRSNYDNLMNYL
                     RI"
     gene            3489..>4426
                     /locus_tag="PK977_12495"
     CDS             3489..>4426
                     /locus_tag="PK977_12495"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRF18984.1"
                     /translation="MLQVRKYRPLLIVALCWCCQLPAQQFTDQSLQADTTVITGEKDA
                     AVFTIGNIIISGNKKTKDFIILREIPFRSGEEYKLQDLVKKFEDARKQLMNTTLFHTV
                     VVAAQNFEGDKVNVSVTVRERWYLFPLPYLKPVDRNLNQWIVEQKASLSRVNYGVKLL
                     YNNASGRNDKLRLWLINGYTKQVSFSYDRLYIDKALKWGMKVSFAAGKNREVNYNTIK
                     DKQVFLKDNENYVRSFVNTSAELTYRRAIKTRHSFGIGYIKEEVGDTIVALNPSYFKS
                     GRNSIHFPELYYTMVYYDLDYIPYPTKGYAAMVSVG"
     assembly_gap    4427..4474
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4475..4927
                     /locus_tag="PK977_12500"
     CDS             <4475..4927
                     /locus_tag="PK977_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18985.1"
                     /translation="SWHFSPKTFFNLNVYGGLKLPFKQPYFNQRFLGYGDVFMQGYEY
                     YVIDGVAGGYIKTTFTREILNFNIKTPNIKGKRAERIPVRIFAKAYGNAGYVHNPQPG
                     DNLLSNKMLYSGGFGIDILTFYDVTFRFEYSFNQLGQSGLFIHRKTIF"
     gene            4931..5809
                     /locus_tag="PK977_12505"
     CDS             4931..5809
                     /locus_tag="PK977_12505"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRF18986.1"
                     /translation="MKAAVYSRVFEDEQQKDVQLFFNELAIQKIEPVIYQDFFEQIKN
                     KIDLPAATTIFSDSDDLTEETEFIISLGGDGTLLDTITMVKGKPVAIMGINFGRLGFL
                     ASIGREQVQIAVKAIANRTYVVDNRTLIHVDASIPMFGNVPYALNEFSIHKRDVASMI
                     KIHTYLNGEFLNTYWADGLLVATPTGSTGYSLSCNGPIVFPDSGSFVITPVAPHNLNV
                     RPIVVPDNNIISFEIESRSDQIICALDARREIVSKNVQLAVRKESFDVHLVRLSENNF
                     LQTLRNKLTWGLDKRN"
     gene            5878..>6276
                     /locus_tag="PK977_12510"
     CDS             5878..>6276
                     /locus_tag="PK977_12510"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF18987.1"
                     /translation="MKKIIAVLIAGIVIVNTGSLRAQESVVQEGEFGIGVGAGHYFGD
                     LNTRARLNRVKMAGTVFFRKNLGNYIAVRVGASFAQLGYSDRYNTHNKYMFSRNLSFN
                     TKVWELTLQGDFNFFRFMPGDPAFSFTPYIT"
     assembly_gap    6277..6392
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6409..>6642
                     /locus_tag="PK977_12515"
     CDS             6409..>6642
                     /locus_tag="PK977_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF18988.1"
                     /translation="MALCFPIGGGFKYALNDRINIGFEVLHRITNTDYIDDVSTTYVE
                     SSLFPANPDGSTSNGLLLSDRSYELGTPIGIPGR"
BASE COUNT         1949 a         1245 c         1444 g         1822 t
ORIGIN      
        1 tccatgcagg agatagctaa tgaggcagcg ttatatgcta aaccggaaag tcctgccgat
       61 attgcggaga aaatgatgct gttgtataaa gatgaaaaac tccggagcca gctgatcgaa
      121 aaaggaagag tggttgtcaa gcaatatagc tgggaacaaa gtgctgaacg cctctggcag
      181 agcattgaaa aagccataca gcaaaacaat taactttgcc acctcttaac gctttatatg
      241 aataaatcaa ctataacaat agaagtagca acggatgaaa acaaaattcc tgataccata
      301 acctggagtg ctactgattc ctctgcagaa aacggacaga aggccaaggc aatgatgatc
      361 tccttttggg atggcgccga gaaggcggct ttacgtattg acctctggac aaaagatatg
      421 atggtagatg aaatggcgga tttcttttac cagacattga tgacgatggc cgatacctat
      481 ggccgtgcta ccaagtacca ggaagatgtg gcggagatga aagattttgc caaaacgttt
      541 tatgaaaagt tcagggctaa gcaactgaag gaaaataaag catagaaaaa gtcctgaggt
      601 tcaggtccta tttgcgacta caacaactaa agaacaatat caatctctaa atgaatatta
      661 tgagtctcga gcaaaagatc atgacggagt taaaggctgc gatgctttct aaagatgaag
      721 cagccttgcg cagtctccgt gctgtaaagg cagcgatatt attagctaaa acctctgaag
      781 gtggcggcgg tgagctgaaa gaagaagacg aaataaagct tttgcaaaaa ctggttaagt
      841 cgagaaagga ctctctcgaa atctatcagc aacaaaaccg tcctgatctg gccaaaaaag
      901 aagaagagga aatagctgtg atagagaagt ttttgcccaa acaactttcc ccggaagaaa
      961 tcaaaacagc attggcttca attattgcag aaacaggcgc atcttctcct gctgatatgg
     1021 gtaaagtaat gggtgtggcc accaaacaac tggctggtaa agcggacgga aagacaattt
     1081 ctgctttggt aaaggaatta ctttcaaagt agtttactat ccggtttaag gatactttaa
     1141 gacaaataat ctcgaataac gtaatttttg acgtttaagc taaaaccttt tcatgctgct
     1201 tgatattgtt tttgctgttg taatggtgtt ggctatcatc aaaggttatc agcggggcct
     1261 tgttgtaggc cttttttccc ttgtatctgt aattatcggc cttgctgctg cagtcaaact
     1321 ttctgcagca gtagcaggat atattggtga ggctgtaaag gtatccgaag agtggttgcc
     1381 ggtcattgct tttgccgtag ttttcatagt ggtagtgatt ttaatacgac ttggtgccaa
     1441 tgctatagag aaagcggtag aggtagtaat gctgggatgg ttgaataaac tggcaggtat
     1501 tctactatat gttactatat atacgatcgt attcagtgtg cttctttttt atgctgaaca
     1561 aatcaaactc attcagcccg ccacaataga aaaatctctt acctattcct acatacagcc
     1621 ttgggggccg aaagtgattg atggattagg ttctctcatt ccctttttta agaatatgtt
     1681 cgacgaactg agtactttct tcagcggagt agcagataat atnnnnnnnn nnnnnnnnnn
     1741 agataatatt tcgaaataaa taagacggat tctggttttt gtataattat ttaatgctga
     1801 aaaatgcaag tcagcgatta ttggtattta aaacccgtca cttgccccta tatttgcctg
     1861 tatcagccct atgttatggg actaaatcgc caattttttg ctgtaaaacg actattttag
     1921 caaccgggtt taagattaga ttgactgctc atggagttcg aaatcaaaca acaagataag
     1981 ttcaagttta ttgaggaagg caatggcgag ccattggtgc ttttgcatgg cctttttggg
     2041 gcgttgagca atttttcaag cctgattcag tatttccgcc agcataacag ggttatcgta
     2101 cccatgcttc cattgctgga aatggatatt ctgcatacat cggttggtgg gcttgccaag
     2161 tttgttcaca aattcctgga agcaagagat cttaggaatg ttcacctgct tggtaattcg
     2221 ttaggtggtc atgtggccct ggtgcataca ttgaaacacc cggagcgcat caaatcgcta
     2281 attcttaccg gcagttctgg tctgtttgaa aatggtatgg gtgacagtta tcccaagcgg
     2341 ggtgactatg aatatatcaa acggaaaaca gagctgacct tttatgatcc caatacagcc
     2401 tccaaggaac ttgtggatga agtatacagt atagtaaaca accgcatcaa ggcgatcaag
     2461 atcatagccc ttgcaaaaag tgctatccgt aataatttag gcgaagaact gaatcagatc
     2521 aagcaaccga ccttgctggt ttggggaaat aatgacacta ttacgccgcc gtttgttgcc
     2581 aaagagttca acaaacttat acccaacagc gaattgtatt tcgtagataa atgtggccat
     2641 gcgcccatga tggaagtgcc ggatgagttc aatgctatcc tgcataaatt tttaaaaaag
     2701 ctgaatgagc ctgcaacagt tgcctgatcg ctgttgtctt gttctgaaaa acgccgctat
     2761 ccatgttaac cggagaacta cattctcaat cactccctta cctgcatctt catgacaagg
     2821 tgtaccaggc attgcaacta atgaatgaca accatgttac ccacttacct atagtagaag
     2881 gagagaagta tatcggatta gtgagtgaag aggacctgat gcaggcggat aatgataatt
     2941 cacctctgga agagcttcag cagtcattct ccggcatttc tgtaaaaaca aatgaacact
     3001 ttttgaaggc tttacaaata gctgcggaaa acgggttgag tgtagtgccg ctggtgaatg
     3061 atgaaaacga actcaccggt acggtggtgt ataatgactt gcttaagtat gcgtccggct
     3121 ttatgagtct aaatgaaccc ggcgcattga ttgtactgga gattcctagc aaccagtatt
     3181 cattcaacga gatcagcaaa ctggtagaaa ccaatgatgc acagattaca cagctaaaca
     3241 cttctaatga tattgaatcg ggtatgatgc aggttaccat ccggatcaat aaaccagaag
     3301 tgtcagatat cattgctact tttcagcgat atgattacaa cgtaaaatat ttttttggag
     3361 aggagttgta taccaacgaa ctgcgcagca attacgataa cctcatgaat tatctccgga
     3421 tctgattgat ttcgctaaga aaagcggata aaactggtta atttagctac ctccgtccat
     3481 ttatggtcat gttgcaggtc aggaaatatc gtcccttact gatagttgcc ctatgctggt
     3541 gctgccagct gcctgcacag caatttactg atcaatcatt gcaggcagac actacagtaa
     3601 taacagggga aaaagatgct gctgtcttca ctattggcaa catcattatc tctggcaata
     3661 aaaagacaaa ggattttatc atactgcggg aaattccttt ccgttcgggt gaagaataca
     3721 aactgcagga cctcgtaaaa aagtttgagg atgcccgtaa gcaactgatg aatacaaccc
     3781 tctttcacac agtagtggta gcggcacaaa actttgaagg tgataaggtg aatgtttccg
     3841 ttacagtcag ggaacggtgg tacttgtttc ccttacctta tttaaagccc gtcgacagga
     3901 acctgaacca gtggattgtt gaacaaaagg ccagcctgag ccgggtgaat tatggagtaa
     3961 agctattgta taataatgcc agcggacgaa atgacaaact aagactgtgg ctgatcaacg
     4021 gttataccaa gcaggtttca tttagttatg acaggctgta tattgataaa gcattgaagt
     4081 ggggaatgaa agtttctttt gccgcaggga aaaacaggga agtaaactat aatacaataa
     4141 aggataaaca ggttttttta aaggataatg agaattatgt ccgaagcttt gttaatactt
     4201 ctgcagagct tacctatcgc agggcgataa aaacccgtca cagttttggt attgggtaca
     4261 taaaagaaga ggtaggtgat actatcgtag cattaaatcc ttcttatttt aaatccggaa
     4321 gaaacagtat acattttccc gagttgtatt atacgatggt ttattatgac cttgattaca
     4381 taccctatcc aacaaagggc tatgcagcga tggtatcagt tgggaannnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagctgg catttttcac ccaaaacatt
     4501 ttttaacctg aatgtctatg gtggtttgaa gctgcctttc aaacagcctt attttaacca
     4561 acggtttctc ggttatggag atgtttttat gcagggctat gaatattatg taattgatgg
     4621 cgtggccgga ggttacatta aaactacatt cacaagggaa atactgaatt ttaatatcaa
     4681 aacaccaaac attaaaggta agagagccga acgcataccc gttcgcatat ttgcaaaagc
     4741 atatggcaat gcgggatatg tccacaatcc tcagcccggc gataacctgc tgagtaataa
     4801 aatgctttat tccggaggtt ttggtattga tatattgacc ttttacgatg ttacattccg
     4861 ctttgaatat agttttaacc agttgggcca aagcggccta tttatacacc gcaagaccat
     4921 attttaattc atgaaagcag ccgtttacag cagggttttt gaagatgaac agcaaaaaga
     4981 cgttcagtta ttttttaatg aactggcaat tcagaaaatt gaaccggtta tataccaaga
     5041 tttttttgaa cagataaaga ataagattga tctccccgca gccaccacaa tattttccga
     5101 ctcagatgac cttactgaag aaacagagtt cattatcagc cttggtggcg atggtacttt
     5161 attagatacc attacgatgg taaaaggcaa accggttgct ataatgggca tcaacttcgg
     5221 ccggcttggg ttcctggcca gtattggcag ggagcaggta caaattgcag taaaggctat
     5281 tgcaaacagg acctatgttg ttgacaaccg aacattaatt catgtagatg caagcatccc
     5341 tatgtttggt aatgtgccgt atgcgctgaa tgagttttcc atccataaaa gagatgtggc
     5401 atctatgatc aagattcaca cctacctgaa cggtgagttc ctgaatacgt attgggcaga
     5461 tggattactg gtagcaaccc ccaccggaag taccggctat tcacttagct gtaatggtcc
     5521 catcgtattt ccggattcgg gtagctttgt cattacacca gtagcgcctc ataacctcaa
     5581 tgtgcggccg atcgttgttc cggataataa tatcatttca tttgagatcg aaagccggtc
     5641 tgaccagatt atttgcgccc tggatgcccg gcgggaaatt gtaagcaaga atgtgcaatt
     5701 ggctgtaagg aaagagagtt ttgatgtgca cctggtacgg ctgagtgaga ataatttcct
     5761 ccaaaccctt cggaacaagc ttacctgggg gctggataaa cgaaactaaa atggcttgtg
     5821 ataaaagaaa atagctactt ttatcgttct atttattccg accatatttt tcgacttatg
     5881 aagaaaatta tcgctgtctt aatagcaggt attgtaattg tgaatacagg tagccttcgg
     5941 gcacaagagt ctgtagttca ggagggtgag tttggtattg gcgtaggtgc cggccattat
     6001 tttggcgacc tgaatacccg ggcccgtctc aacagggtaa aaatggcagg cactgttttt
     6061 ttccgtaaaa acctgggcaa ttatatagcc gtaagggtag gtgccagctt tgcacaatta
     6121 ggttattctg accggtataa tacgcataac aagtatatgt tcagccgcaa cctgagcttt
     6181 aatactaaag tatgggagtt aactcttcag ggagatttta acttctttcg ttttatgccc
     6241 ggcgatccgg catttagctt tacaccatac attactnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaacctt acaaatccat ggccctttgt
     6421 ttcccgatcg gaggtggttt caaatatgct ctcaacgatc gcatcaatat tggctttgaa
     6481 gtactgcatc gtattaccaa taccgactac attgatgatg taagtactac ttatgtagaa
     6541 tcgtcattgt ttcctgccaa cccggatgga agcacttcaa acggcttgtt gctgagcgac
     6601 cgttcttatg aattaggaac acccattggt attcccggtc gc
//
LOCUS       DAORLS010000170         6640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6242_length_6640_cov_1.965680, whole genome shotgun sequence.
ACCESSION   DAORLS010000170 DAORLS010000000
VERSION     DAORLS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6242_length_6640_cov_1.965680"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..2275
                     /locus_tag="PK977_12520"
     CDS             110..2275
                     /locus_tag="PK977_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF18989.1"
                     /translation="MKKLLLILSITVLLFANSTTAQTPLKPRDPSITGPQTFAIVMGI
                     SKYKYVRPLTYADKDAELFRDFLKSPGGGSVKEDNIFCLLNDQALSANFWTKGFQWLK
                     YKQLQKGDRLFIYLAGHGDAIDEDQYFFLGYDCNPAGDKNNYLVGGAIQLYNLKKKIA
                     AETTKGVEVFFVMDACRSNELPGGAEGLSFLNTAVTEKKVGEIMMLATAAGQESLEDA
                     SIGNGHGLFTWYLVDGLSGLADAEGSPDNKITYSEIRKYVDKNVPSVAQQRFRKKQQP
                     YFCCDANSENVISQVDAAYLQKWMQQKKQNRAGDSFEGMLNNAFDFYGADTTLQETYN
                     LFYKAIREKRLTGNASAEYYYQQLDKKYKGNPYTLDAQSTLAAEFINDAQQRVNRYLD
                     CSDEPSAKEKKENYEAGQRLEKAINLVRQDDPDFANALLGRMYLLKSNSDDMAFQNAY
                     AALRIDPNGAYIQNKLALLHLANNKTDSALFYASKAAKTAPKWICALTTLALVQKATG
                     QKPNDVKKNNTPLRKASIGFTAGGGLSQSNPTFSGNVNTGFIGVDAATAPGGNLGIIY
                     QVNLGGSISIRPSATVSFGNTDIDFQRRNVTGGPITTETVSVKSSSVNVSLPLIIRLG
                     NKNVAPYIALGPSFSYMLNGAASADEILPVKKSLFLGDGGLGVDINLLKSGIILSPEL
                     KYSAGLSEWRDDAGTSNYASALSSLKKNGFTLSLYLRKR"
     gene            complement(2282..>3525)
                     /gene="bshC"
                     /locus_tag="PK977_12525"
     CDS             complement(2282..>3525)
                     /gene="bshC"
                     /locus_tag="PK977_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HRF18990.1"
                     /translation="LKTSLPDYNFVPIFYIGSEDADLDELNHIHLGGEKLVWQTKQSG
                     AVGRMKADKDLVKLIGLMEGQLAVLPYGKEIIAMLKEHYKEGASIQQATFQFVNNLFA
                     EYGLVIILPDNPELKRQMIPLFRDELLHQHSATVIEKSAAQLETAGYKVQASGRDINL
                     FYLEDGIRNRIEFKNGQYQVVDTKLVFTSDEILQELESHPERFSPNVILRGLYEETIL
                     PNIVFIGGGGETAYWLQLKDLFAHYAIPFPVLVLRNSFLVVEKKWQELIGKLNLTIED
                     FFLPEQELLNKLVLRDSRNDVKLNGSLDEVEKLYESFKKQAAAIDSTLEKHVDSLKSK
                     TVYRLQELEKKMLRAEKRKFSDQQRQIQTIKSHLFPGNGLQERVDNIAYYYARWGATF
                     LKTLYDNSLGLEQEFAILSEQ"
     assembly_gap    3526..3577
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3578..3897)
                     /gene="bshC"
                     /locus_tag="PK977_12530"
     CDS             complement(<3578..3897)
                     /gene="bshC"
                     /locus_tag="PK977_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HRF18991.1"
                     /translation="MNCTSTRLPYRQTGAFSKIAMDYIDQADALRPFFALPPTLQGLQ
                     KAIENRKQFATNRDELVKELKKQYENVSTTEKVKANIELLSSADTFTITTAHQNNIFT
                     GPLY"
     gene            complement(3963..>4251)
                     /locus_tag="PK977_12535"
     CDS             complement(3963..>4251)
                     /locus_tag="PK977_12535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRF18992.1"
                     /translation="MNRVQGILDAVKRYYPEFDVPLPPKEKIWYGYRPCSADGLPYIG
                     RVKKYSNLTIATGHAMLGLSLGAGTGKLVNEIVNHSALSMDVRPFTVERFS"
     assembly_gap    4252..4320
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4321..>4648)
                     /locus_tag="PK977_12540"
     CDS             complement(<4321..>4648)
                     /locus_tag="PK977_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18993.1"
                     /translation="DAHCYPNKLMSDLIAYLQSSGVQLLSGNEVNGFESCSGRINKVV
                     TSKGTLTADEIVMATGSWGRQTAKLLGINIPLMPGRGYSVTLQDSPYKINYPSVLVEG
                     RVALTP"
     assembly_gap    4649..4666
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4667..>4890)
                     /locus_tag="PK977_12545"
     CDS             complement(<4667..>4890)
                     /locus_tag="PK977_12545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18994.1"
                     /translation="EDWAKMPGFDFAYERKGLLEIFQTQEGADHAHHTMEKAHQLGLK
                     DTVVLSKAELDKLEPQTRINALGALFFKCD"
     assembly_gap    4891..5050
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5051..5246)
                     /locus_tag="PK977_12550"
     CDS             complement(<5051..5246)
                     /locus_tag="PK977_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF18995.1"
                     /translation="MKAIVIGGGIIGLSSALYLRKSGWEVIVLDKGDFSDNCSYGNAG
                     YICPSHFIPLATPGIVKQGFK"
     gene            complement(5262..6194)
                     /locus_tag="PK977_12555"
     CDS             complement(5262..6194)
                     /locus_tag="PK977_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF18996.1"
                     /translation="MSASVAGCGQLSKDNASPKNKNLPELFLDVLGIAQDAGFPQAGC
                     EKECCAAYYSGKEEKKLVTCLALVDRATNKYWLIEATPDIATQLKNLQAFLPATDYSP
                     EGIFITHAHIGHYTGLMQLGREAMGVKDIPVYVMPRLDSFIRNNGPWSQLVSLQNISL
                     RSLNADSTVHLNASFKITPVKVPHRDEYSETVGFIIQSDKKKVLFIPDIDKWEKWARD
                     IVAQIGEVDFALLDGTFYKNGELPGRDMSEVPHPFVEESLQKFSALSSVDKSKIIFIH
                     FNHTNPLLKNKSAEKENVIKAGFAVAEEGMIIEL"
     gene            complement(6230..>6640)
                     /locus_tag="PK977_12560"
     CDS             complement(6230..>6640)
                     /locus_tag="PK977_12560"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HRF18997.1"
                     /translation="GRTYRYFSGEVQHPFGFGLSYTSFSYSGLKVITEKNGFSIQLDV
                     QNTGKYAGDEVVQLYIRRVSAGDNEPLKQLKDFKRVSLAKGETKKVNLHLNRQDIAWY
                     DESVKKWIVYPGAYELVIGGSSEDKKMVYHLTIK"
BASE COUNT         1800 a         1459 c         1272 g         1810 t
ORIGIN      
        1 acaatggaag cagcaccctt ttccgggtca ttgtccagaa ggattaagca ggctgtcaga
       61 aattctgtat tttactgagc aatcaaccta accaaaaccg tccggtaata tgaaaaagct
      121 attgctcatt ctgtcaataa ctgttttgct gtttgcaaat tccactactg cacagacacc
      181 actaaaaccc cgtgatcctt ctataaccgg accgcaaact tttgctattg taatgggtat
      241 ttccaaatac aaatatgtcc ggccacttac ctatgcagat aaagatgccg agttgttcag
      301 ggacttttta aaatctcccg gtggcggtag tgtaaaagag gacaatattt tttgcctgct
      361 gaatgaccag gctttaagcg ccaatttctg gacaaaggga tttcagtggc tgaaatacaa
      421 acaacttcaa aaaggtgacc ggctttttat ttacctcgcc ggtcatggtg atgcgattga
      481 tgaagaccag tatttcttct tgggttatga ttgtaatcct gccggcgaca aaaacaatta
      541 ccttgttggc ggcgctattc aactatataa cctgaaaaag aaaatagctg ctgaaactac
      601 taaaggagta gaagtgtttt ttgtgatgga tgcctgccgc tccaacgaat tgcccggcgg
      661 tgctgaagga ttgagctttt taaacaccgc tgttacagag aaaaaagttg gcgagatcat
      721 gatgctggca accgcagccg ggcaggaatc gctggaagat gcctccattg gtaacgggca
      781 tggtttgttt acctggtatt tggtggatgg tttaagcgga ctggctgatg ctgaaggaag
      841 cccggataac aagatcactt attcagagat caggaaatat gtagacaaga atgtaccttc
      901 tgtggcgcag cagcgtttca ggaagaaaca acaaccttat ttctgctgtg atgccaatag
      961 cgaaaatgtc atcagccagg tggatgccgc ttatctgcag aaatggatgc aacaaaaaaa
     1021 acagaaccgg gcaggagatt cattcgaagg aatgctgaat aacgcatttg acttttacgg
     1081 ggcagatact accctgcagg aaacatataa cctgttctat aaagccatca gggaaaagag
     1141 attgactggc aatgcatcag cagagtatta ctaccagcaa ttagataaaa aatacaaagg
     1201 caatccttac acactggatg cacaatcaac gctggcagct gagtttatca atgatgcaca
     1261 gcaaagagtg aatcgttacc tggattgcag cgatgaacct tctgccaaag agaaaaaaga
     1321 aaactatgaa gcaggacaac gactcgaaaa agcaattaac ctggtaagac aagatgaccc
     1381 ggattttgcc aatgccctgc ttggcagaat gtacctgctc aaatcaaaca gcgatgacat
     1441 ggcttttcaa aatgcatatg ctgcgctgag gatcgatcca aatggtgctt atatacaaaa
     1501 caagctggcg ctgctgcacc ttgctaataa taaaacagat agtgccttgt tttatgccag
     1561 taaagccgcc aaaactgctc ccaaatggat ttgtgcatta accaccctgg cattagtgca
     1621 aaaagcaaca ggtcaaaagc caaacgatgt aaaaaagaat aatacaccgt taagaaaagc
     1681 cagtattgga ttcactgcag gcggtggatt aagccagtca aacccgacat tctctggcaa
     1741 tgtgaatacc ggctttatcg gtgttgatgc agctaccgcc cccggcggca acctcggcat
     1801 catttaccag gttaatcttg gtggcagtat ttctatcagg ccgtctgcaa cggtaagttt
     1861 tggcaatacc gatatcgatt ttcagcgccg gaatgtaacc ggcgggccca ttacaacaga
     1921 aacagttagt gtgaaaagca gttcagtcaa tgtttcgctt ccattgatta taaggctggg
     1981 caataagaat gttgctcctt atatcgctct gggtccttca ttcagttata tgcttaatgg
     2041 tgcagcctca gcagatgaga tactgccggt aaaaaaatca ttattcctcg gcgatggcgg
     2101 attgggtgtt gatatcaact tgctgaaatc aggaatcata ttatcaccgg aattaaaata
     2161 ttcagccggc ctgtcagaat ggagagatga tgctggtacc agcaattatg ccagtgccct
     2221 gtcatcattg aaaaagaatg gatttacatt gagcctttac ttaagaaagc ggtaataatt
     2281 tctactgctc agataagata gcaaactcct gttcaagacc aagggagtta tcatagaggg
     2341 ttttgagaaa tgttgcaccc cagcgggcat agtagtaagc tatattatct accctttcct
     2401 gcaaaccatt accaggaaac aggtgcgatt taattgtttg tatctgccgc tgctggtcac
     2461 tgaactttct tttttcagcc cgcagcattt tcttttccag ttcctgcagg cgataaactg
     2521 ttttggattt caaagaatcc acatgttttt ccaacgtaga atcaatagct gctgcctgtt
     2581 tcttgaatga ttcatatagt ttttctactt catccagtga gccgtttagt ttcacatcgt
     2641 ttctgctgtc acgaagcacc agtttattga gtaattcctg ctcgggcaaa aagaaatcct
     2701 caatcgtcaa attcaattta ccaatcagct cctgccattt tttctctacc actaaaaagg
     2761 aattgcgaag aaccaataca ggaaaaggaa tagcataatg ggcaaacaag tctttcagtt
     2821 gcagccagta ggccgtctcg cctcctccgc cgataaacac aatattgggt aaaatagttt
     2881 cctcatacaa tccacgcagt attacgttag ggctaaatcg ttccgggtgc gattccagtt
     2941 cctgtaaaat ttcatcgcta gtaaagacaa gtttcgtatc cactacttga tattgtccat
     3001 tcttaaactc tatccggttt cggattccat cttccaggta aaataaattt atatcccggc
     3061 cactggcctg cactttataa ccggctgttt caagttgcgc cgccgatttc tcaatgactg
     3121 ttgctgaatg ctggtgcagg agttcatccc tgaaaagagg gatcatttgt cttttcaatt
     3181 ccgggttatc aggcagtatt atcaccaacc catattcagc aaataaatta tttacaaact
     3241 gaaaagtcgc ctgctgaata cttgctccct ctttgtaatg ctctttgagc atcgcaatga
     3301 tctctttacc atacggcaat actgccagtt gcccttccat cagcccaatc agctttacca
     3361 ggtccttgtc cgctttcatc cggcccacag cgccgctttg tttggtttgc cataccaatt
     3421 tctcgccgcc aagatgaatg tgatttaact catccaggtc tgcatcttcc gagccgatat
     3481 aaaaaatggg tacaaagttg taatcaggca aacttgtttt taatgnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa tacagcggcc cggtaaaaat
     3601 attattctgg tgagccgtgg tgatggtaaa tgtatcagcc gatgataaca attcaatatt
     3661 tgcctttact ttttcagtag tacttacatt ctcatactgc ttctttaatt ccttgaccag
     3721 ctcatcccgg ttggtggcaa attgttttcg gttttcgatt gctttttgca agccctgcaa
     3781 ggttggcggc aacgcaaaga aaggcctcaa agcatcagcc tggtcaatat aatccatagc
     3841 aatcttcgag aaagctcctg tctggcggta aggcaagcgg gtagaggtac aattcatacg
     3901 aggctgtaaa agtaacccac agattggaat gattgaaatg atattaactg ctgacgggta
     3961 ttttatgaaa acctttcaac agtaaaaggc cgaacatcca tactcaaagc ggaatggttg
     4021 acaatttcat tcaccagctt accggttccg gcgcccaaac tcaggcccag catggcatgc
     4081 ccggtggcaa tagtaaggtt gctatatttt tttactctcc cgatatacgg caaaccatct
     4141 gcagaacagg gccggtagcc ataccatatt ttttcttttg gaggcagcgg tacatcaaat
     4201 tcaggataat atctcttcac tgcatccagt attccctgaa cccggttcat cnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 atcggggtaa gtgccacccg cccttccacc agcactgatg gatagttgat tttataagga
     4381 gaatcttgca acgtaacaga ataacccctg cccggcatca gcggaatatt tatgcccagc
     4441 agttttgcag tttgtcttcc ccatgaaccg gtagccatca ctatttcatc agctgttaaa
     4501 gtaccttttg atgtaactac tttatttatt cttcctgaac aggattcaaa gccatttact
     4561 tcattgccac tcaataactg cacaccggat gactgaaggt atgcgatgag atcactcatc
     4621 agtttattgg gataacaatg tgcatcacnn nnnnnnnnnn nnnnnnatca catttaaaaa
     4681 ataaagcacc aagagcattg atccttgtct gtggttccag cttatccaat tcagctttac
     4741 ttaataccac tgtatccttc aagcctagct gatgtgcttt ctccattgta tgatgtgcat
     4801 ggtctgctcc ttcctgtgtc tggaaaattt caagcaggcc tttccgctca tacgcaaaat
     4861 caaacccggg cattttggcc cagtcctcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn atttaaaacc ctgctttaca atgccaggtg ttgctaaggg gatgaagtga
     5101 ctggggcata tataaccggc gttgccataa gagcagttgt cggaaaaatc acccttatca
     5161 agcacaataa cttcccagcc tgattttcgt aaatacaaag ccgaacttaa accaataata
     5221 cctccgccga ttacgatcgc tttcatgatt aatgagcaac tttatagttc tatgatcata
     5281 ccttcttctg caactgcaaa accggcttta atcacatttt ctttctctgc agacttattc
     5341 ttcagcaacg gattggtatg attgaaatga atgaatatga tttttgattt atccaccgaa
     5401 gatagggccg aaaatttttg caggctctct tccacaaaag gatgaggcac ttcactcata
     5461 tcccttccgg gtagttcgcc gttcttataa aatgttccat caagcagggc aaaatccact
     5521 tctccgattt gtgcaacaat atccctcgcc cacttttccc atttgtcgat atccggaata
     5581 aataacacct tctttttatc actctggatt atgaagccaa ctgtttccga gtattcgtca
     5641 cgatgcggca cttttactgg tgttatttta aaagaagcat tcaaatgaac agtgctgtct
     5701 gcattcaaac ttcttaaact aatgttttgt aatgagacta actggctcca tggaccattg
     5761 ttacggataa aagaatcaag ccggggcatt acatacaccg gaatgtcctt tactcccatt
     5821 gcttcccttc ccagttgcat gagtcctgtg taatgaccaa tatgtgcatg agtgataaag
     5881 atcccttctg gagaataatc agttgccgga agaaaggcct gaagattctt caactgcgta
     5941 gcaatatcag gagtagcttc tatcagccag tatttgttgg ttgccctgtc caccaacgcc
     6001 aggcaggtaa ccagtttctt ttcttccttc cccgagtaat aggcagcaca acattctttc
     6061 tcacagcctg cctgcggaaa accggcatcc tgtgcaatcc ctaatacatc caaaaagagc
     6121 tccggtaaat ttttattctt cggtgaagca ttatccttac tcagttgccc gcagcctgct
     6181 actgatgctg acacagccaa taataatatc caaattgttt ttttcaggat catttgattg
     6241 ttaagtgata taccattttt ttatcttcag aagaaccacc gatcaccagc tcgtacgcac
     6301 cggggtagac aatccatttt ttgactgact catcatacca ggcaatatcc tgccggttca
     6361 aatgcaggtt tacttttttt gtttcccctt ttgccaggct cacccttttg aaatctttta
     6421 attgcttcag cggttcatta tcgcctgcac tcacccggcg aatgtataac tgcaccactt
     6481 catcgcctgc atacttaccg gtgttttgca catccagctg aatggaaaaa ccattctttt
     6541 cggttattac tttcagtccg ctataagaaa agctggtata acttaagccg aatccaaaag
     6601 gatgctgcac ttctccgcta aaatagcgat aggttcttcc
//
LOCUS       DAORLS010000171         6488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6434_length_6488_cov_1.894295, whole genome shotgun sequence.
ACCESSION   DAORLS010000171 DAORLS010000000
VERSION     DAORLS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_6488_cov_1.894295"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK977_12565"
     CDS             <1..195
                     /locus_tag="PK977_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRF18998.1"
                     /translation="LVGPAYRDVANKYELTNDNVNMLAEKVVKGGSGVWGEVAMPANT
                     VISPEDAKAAVRYVLMLRNK"
     assembly_gap    211..241
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            368..1030
                     /locus_tag="PK977_12570"
     CDS             368..1030
                     /locus_tag="PK977_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRF18999.1"
                     /translation="MKTVLIAFSISLIFLNGTGCRPMQQETALDNPVKQLLDGNNRFA
                     NMHALHPHETAARLREIAEGQHPFAAVVCCSDSRVPPEVVFDQGLGDLFVVRTAGNLM
                     GGLEIGSIEYAVEHLGVKQVVVMGHKECGAIKAFAEGGEVPGHIKDIVDSIKAEKEIQ
                     LIPANDQHLLDDCIRANILHGIHQLKQQSPIIAEKIRKGELQICGACYDIRRGIVELV
                     KE"
     gene            1177..>1802
                     /locus_tag="PK977_12575"
     CDS             1177..>1802
                     /locus_tag="PK977_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HRF19000.1"
                     /translation="MIHPFSYVSLEDAVNIIQPGHRVFVHGSACTPLPVLHEMARQHE
                     RLKGVEVVSITFRGDIMIDKPEYYDSFKINSLFVSASVRKAVNEGRADFIPVFLSDIP
                     DLFKRKLLELDVAIVQVSPPDVHGYCSLSLSIDIARSAVDSAKYIIALVNPRLPRTHG
                     DGMIHTDRFTKMVFHESEPPEENYGDKTGEAELTIGQNIAGLIDDGST"
     assembly_gap    1803..1847
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1848..2477
                     /locus_tag="PK977_12580"
     CDS             <1848..2477
                     /locus_tag="PK977_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRF19001.1"
                     /translation="KCLGNHKNLGVHTEMLSDGIIDLVEKDVINNKLKRVHPNKTVTS
                     FAVGTRKLYNYIDDNPSFAFLDIDYVNDPHVIRRNPRVIGINSAIEVDITGQVCSDSI
                     GEFQYSGIGGQMDFMRGAALSEGGKPIIALTSRTAKGIPRIVPTLKQGAGVVTTRGHV
                     HYVATEYGIAFLFGKNFRQRAKALINIAHPDDRENLERYCHERFKIFSL"
     gene            complement(2546..2956)
                     /locus_tag="PK977_12585"
     CDS             complement(2546..2956)
                     /locus_tag="PK977_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19002.1"
                     /translation="MKKIFRNNRIIAIAFMTFFSASAFATPKAGEPEKDVPVSLSYAG
                     NIQEQPLFQLSFFGNKENDDFTIIISDTWGNRLYRENIKAENFSKKFLLNTEEIGDET
                     LTFEVISNKTGKKVQYEINRFTRTGAIALAMVSK"
     gene            3556..>3917
                     /locus_tag="PK977_12590"
     CDS             3556..>3917
                     /locus_tag="PK977_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRF19003.1"
                     /translation="MAFKKDDIIIIEPKEVFVGKKDAAVTLMEFGEYESEECAKANEI
                     VKQLLEDYEGKIRFQFRHFPLTLIHQRSLKASESAVAAAQEGKFWEMHNVLFHNRRNL
                     GTTSLKLYSKEAGVKNKK"
     assembly_gap    3918..4039
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4217..4510)
                     /locus_tag="PK977_12595"
     CDS             complement(4217..4510)
                     /locus_tag="PK977_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRF19004.1"
                     /translation="MNIYVSNLSFAVVDEDLRSYFAEYGEVTSAKVIMDKFTNRSKGF
                     GFVEMSDDAAAQKAIAELDGATVDGRSIKVSVAKPREERPARSSRPTYSNNRW"
     gene            complement(4723..>5358)
                     /locus_tag="PK977_12600"
     CDS             complement(4723..>5358)
                     /locus_tag="PK977_12600"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF19005.1"
                     /translation="YEANISVDERPILAAGINETKDIFTNVIYQADSSRSQAYRTYDN
                     LGRNKEWYLRGLGAIPPGGRYFFVIGAQYNHNFYQGFYEGKPLSFKRGTWTFFTYQTF
                     KVDKRSVITLNGFYRLKGQQQFYELSEFGALNASINRKFLKDKLILTLSINDMFYTNK
                     NDFSIQQGSVNAAGTRLADTRRWGINIRYNFGIKKKEENNNLFNAEPPVSN"
     assembly_gap    5359..5474
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5475..>6488)
                     /locus_tag="PK977_12605"
     CDS             complement(<5475..>6488)
                     /locus_tag="PK977_12605"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF19006.1"
                     /translation="GVVNVVLKKGVRIGMTGSVNAGMQQGTYGNQFIGFNINNNDGRK
                     NSNFNFNYSRRNSFEQIITDRIFASDSMLSQDAYTKYPGSNLYGSFGITYDLTKKWEL
                     TYDATASYNQFDNNSENINNLFKISTGEAFSNSVNKVNNDGSNFSIGTGFETKYKIDS
                     LGSEWTTDTWYNHTINKSNQLFSTRYDSPIPVLFGGDGIANSDRDYFNFRSDLKLKMK
                     NKFTFEAGLQSYINNFYNRAKYFREANGTRTDDKSRTNSFRSNENINALYLQGSKTIG
                     KDFVAKFGTRLENTNLRGRQLIPSDTSFAIHRSDLFPYIYLSKNLMKIAGFDLRAYLI
                     YRRS"
BASE COUNT         1842 a         1178 c         1294 g         1860 t
ORIGIN      
        1 ctcgtagggc ccgcatacag ggatgtggca aataagtatg aactaaccaa tgataatgtg
       61 aatatgcttg ctgagaaagt ggtgaaaggc ggatcaggtg tatggggtga ggtggccatg
      121 cctgccaata cggtaatatc tcctgaagat gcaaaagcag cagttcgcta tgtactgatg
      181 ctgagaaata aataaagctg atttagctgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 natccatgaa atccggcttt tttattgctg acgaaactca tttttcatcc tgagcagctt
      301 catcagaccc gggatcagca ggcaataatt tcgccggcaa ctgtttctga actaaatccc
      361 ccgcatcatg aaaacagtct taatagcttt ttctatttcg ttgattttcc tgaatgggac
      421 aggctgtcgc cctatgcaac aggaaactgc attagataac ccggtaaaac aattactgga
      481 tggaaataat cgcttcgcca atatgcatgc attgcatccg catgaaacgg cagcaagact
      541 cagggaaatt gctgaaggtc agcatccctt tgcagctgtg gtttgttgtt cagattcaag
      601 agtacctccc gaagtagtat ttgaccaggg acttggcgat ttgtttgttg ttcgtacagc
      661 agggaacctg atgggaggcc ttgagattgg cagtattgaa tatgcggttg aacacctggg
      721 tgtaaaacaa gtggtggtaa tgggacataa agaatgcggc gctatcaaag catttgcaga
      781 aggaggtgag gtgcccggtc atatcaagga tatcgttgac agcattaaag cagagaaaga
      841 aattcaactt attccggcga atgatcagca tctgttggac gattgtatcc gggcaaatat
      901 tttacatgga attcatcagt taaagcaaca atcacccatt attgctgaga agatcagaaa
      961 aggtgaatta caaatatgtg gtgcatgcta tgatatccgc cgcggtattg tagaactcgt
     1021 aaaagaatag cctcattttt ttggcgagaa aaattaatct cataagctgt catttattct
     1081 gactttcgct gtacaaaatc atactatggg ctgatattaa tcttagctct catttcagaa
     1141 atttcggtac ttcgcatcat aaataatctt acgaaaatga ttcatccgtt tagttacgtt
     1201 tccctggaag atgctgttaa tattattcag cccggtcacc gtgtatttgt tcatggaagt
     1261 gcctgtacgc cattgcctgt tttgcatgaa atggcccggc agcatgaacg cctaaagggt
     1321 gttgaagtag tctccataac ctttcggggt gatattatga tcgacaagcc tgagtattat
     1381 gattctttca agataaactc actgtttgta tctgcttctg tccgcaaggc agtaaatgaa
     1441 ggaagggcag attttatacc tgttttttta agcgatattc ccgatctttt taaaagaaag
     1501 ttgcttgaac tggatgtcgc tattgttcag gtttctcctc ctgatgtgca tggttactgt
     1561 tcactgagct tgtccattga tattgcccgt tcagcggttg atagtgctaa gtacatcatt
     1621 gccctggtta atcccaggct tccacgtaca cacggggatg gtatgataca cactgaccgt
     1681 tttaccaaga tggtattcca tgaatcagaa ccacctgaag aaaattacgg tgataaaaca
     1741 ggtgaagcag agttgaccat cggtcagaat atcgcagggc taatagatga tggtagtacc
     1801 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa tgccttggta
     1861 atcataaaaa cctgggggta catacagaaa tgctgagcga tggcatcatt gacctggtag
     1921 agaaagatgt gatcaataat aaattgaaaa gggtacatcc caacaaaacc gtgacttctt
     1981 ttgcagtggg tacccgtaag ctgtataatt atattgacga caacccttca tttgccttcc
     2041 tggatatcga ttatgtaaat gacccgcatg taatacgccg caatcccaga gtgattggta
     2101 ttaacagcgc tattgaagtg gatataaccg ggcaggtttg ttcagacagt attggcgaat
     2161 ttcaatacag tggtattggc gggcaaatgg actttatgcg tggtgctgcc ttaagtgaag
     2221 gtggaaaacc gataatcgca ttgaccagtc gaactgcaaa gggtattccc cgtatcgttc
     2281 cgacattaaa gcagggcgcc ggtgttgtaa ccaccagggg acatgtgcat tatgtggcta
     2341 cagaatatgg tattgcgttt ctttttggta aaaacttccg ccagagagca aaggcgctga
     2401 tcaatattgc gcatcccgat gacagggaaa acctggagcg gtactgccac gaaaggttta
     2461 aaatattcag tctctgataa tagtagtaag acaaaaggag atgctgtaag caaattacag
     2521 catctccttt tttttggtgt gtaaattatt ttgaaaccat agcaagggct atggcacctg
     2581 tacgggtaaa acggtttatc tcgtactgta ctttcttgcc agttttattg ctgataactt
     2641 caaatgttag cgtttcatca ccaatctctt ctgtattcag cagaaatttt ttactgaagt
     2701 tttcagcttt tatgttttca cgataaagcc tgtttcccca tgtatcacta atgatgatgg
     2761 taaaatcatc attttcctta tttccaaaaa agcttaattg gaaaagaggc tgttcctgta
     2821 tgttgcctgc ataactcagc gaaacaggaa catctttttc tggttcacct gcttttggcg
     2881 ttgcaaaagc agaggccgag aaaaatgtca taaatgcgat cgcaatgatg cggttgttac
     2941 ggaagatctt tttcatggtt atattatttt agttacaaaa aacaattcat tatttcctat
     3001 gcgcaggcaa tctggcaagc aataaaaatt gggcaaacaa aagggcaagt gcagttatcg
     3061 gatcatagcc agatttggcg aaagcaatta aatattgctt tgatgatgta aaagtagggc
     3121 ggctcacttt tacgctaaaa ccaggttttt agttgtctta ggatttccaa agtaattcga
     3181 ccttgcttaa agactttaaa atcagtcaat tttgtaaaca aagcggcgta cggccaacat
     3241 acattcattt actaatgcta cataccatcg attcaatttc cagcaaccgg agtttgataa
     3301 aatgtcattt tagcattttg caaaagatcc ttttaattga tgtctaaaca tttgataatc
     3361 aggactcctg tttctttttg gtaaattaat taacagtgga tttggccagg tacaaaaaaa
     3421 tattatcttc aagcgtttac gatacatgtc tgaatggcat aaccgctttt gtttcaacga
     3481 aatggcggaa aatttgttaa gcataataca ggtccatgcc cacataggga tgtggatgaa
     3541 aaaaggagca ttaaaatggc atttaaaaaa gatgatataa ttattattga accaaaagag
     3601 gtttttgttg gtaaaaaaga cgccgctgtc acattaatgg aatttggcga atacgaaagt
     3661 gaggaatgtg cgaaagccaa tgagatagta aagcaactat tggaagatta tgagggaaaa
     3721 atcaggtttc aattccgtca tttcccgctt acgcttattc accagcgcag cctgaaagcc
     3781 agtgaatcag cggttgcagc tgcacaggaa ggaaagtttt gggaaatgca taatgtatta
     3841 tttcataacc gtcgtaatct tggtaccaca agcctgaaat tatacagtaa agaagctggt
     3901 gtcaagaata agaaattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnc gacttatgcc aatatcagcg ctgctattga tgcggcgctg
     4081 aaaaaaagta aatccaaagc agctcctgcc aagaaagctg ctgccaagca aaagcaaaga
     4141 gcttaaaact tagttgtaat aaataaaaaa aaccggttca attgaaccgg ttttttattg
     4201 atcattggtt attttattac cagcgattgt tggagtaagt gggtcttgaa gaccttgcag
     4261 gtctttcttc ccttggtttt gcaacgctaa ctttgataga acgaccatca acggtagcac
     4321 catccagttc agcgattgcc ttttgggcag ctgcatcatc actcatttcc acaaaaccga
     4381 agcccttgct gcggttagtg aatttgtcca taataacttt tgcagaagta acttctccgt
     4441 attcggcaaa ataacttctc aggtcttcgt ctacaacagc gaagctcagg tttgaaacat
     4501 aaatgttcat gtaaaaaaat tgacttttaa aaatacttga gaataacgca agggtaaaga
     4561 agactaacta ttagcagatc cttcgaaaaa cgcaggatta aaagcgtagc agaaaaaatc
     4621 gttcacttac aaaatgctct ataactcaaa ttaacaggac aaagataagg ggattaatcc
     4681 agacgcactg gtttaatttg ttatacttgt ttaacaggtt gattagttgg atacaggagg
     4741 ctcagcgttg aacaaattat tgttttcttc cttctttttt atgccaaaat tgtagcggat
     4801 attaattccc catcttcttg tatcagccaa ccgggttcct gctgcattta cagaaccctg
     4861 ttgaatactg aaatcatttt tattggtata aaacatatcg ttgatgctaa gggtgagtat
     4921 cagtttgtct ttcagaaatt tcctgttaat acttgcatta agagcgccaa attcggagag
     4981 ttcataaaac tgctgctgcc cttttagacg gtaaaagcca ttcaatgtta taacagatcg
     5041 tttatcaact ttgaaagtct gataagtaaa aaatgtccag gttcctcttt tgaatgataa
     5101 tggcttccct tcataaaatc cctgataaaa attatgatta tactgagcgc cgatcacaaa
     5161 gaaataacga cccccgggag ggatggcgcc aagccctctc aagtaccact ctttatttct
     5221 gcctagattg tcataggtac ggtaagcctg gctgcggctg ctgtctgcct ggtaaattac
     5281 attggtgaaa atatcttttg tttcattaat tcctgcagcc agtatcggtc tttcatccac
     5341 acttatatta gcttcatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnngaacgg cggtagatca ggtaagctct cagatcaaag ccggcaatct
     5521 tcatcaggtt tttgctcagg tagatatatg gaaaaaggtc actgcggtga atggcaaagg
     5581 aagtatcgga aggtatcagt tgccgccctc tcaggtttgt attctccaaa cgggttccaa
     5641 attttgctac aaaatccttt cctatcgttt tcgaaccctg caggtagagc gcattgatat
     5701 tttcattgga acgaaaggaa tttgtacggc ttttgtcatc agttctggtg ccatttgctt
     5761 cccggaaata tttagccctg ttgtaaaaat tattaatata ggattgcaaa ccggcctcaa
     5821 acgtaaactt attcttcatt ttcagcttca ggtctgagcg aaagttgaaa taatccctgt
     5881 cgctgttagc tataccgtct ccgccaaata aaacaggtat ggggctgtca tatcttgtag
     5941 aaaatagctg gttggattta ttgatggtat gattgtacca ggtatcagtt gtccattctg
     6001 atcccaacga atcaatctta tattttgttt caaaccctgt tcctattgaa aagttgctgc
     6061 catcattgtt tactttatta acactgttac tgaaagcttc accggtactt attttaaaca
     6121 agttatttat attttctgaa ttattgtcga actggttata gctggcagta gcatcataag
     6181 ttaactccca tttttttgtc agatcatagg tgataccaaa agatccataa aggttgctgc
     6241 cgggatattt tgtataagca tcctggctca gcatggagtc actggcaaaa attctgtcgg
     6301 taatgatctg ttcaaaactg tttcggcgac tgtagttgaa attaaaattt gaatttttcc
     6361 tgccgtcatt attattgata ttgaagccaa taaactggtt gccataggta ccttgttgca
     6421 tacccgcatt tacactaccg gtcataccaa tacgaacacc cttctttaaa actacattca
     6481 ccactccg
//
LOCUS       DAORLS010000172         6400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6555_length_6400_cov_1.693932, whole genome shotgun sequence.
ACCESSION   DAORLS010000172 DAORLS010000000
VERSION     DAORLS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6555_length_6400_cov_1.693932"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PK977_12610"
     CDS             <1..260
                     /locus_tag="PK977_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRF19007.1"
                     /translation="DVKNLVLILKETVSIDIAAAEKLVKIQQQFYENGASFVICELQK
                     QVEDFLDQQELLELMNVTPTESEAWDIVQMEEIERDLMGDE"
     gene            330..1232
                     /locus_tag="PK977_12615"
     CDS             330..1232
                     /locus_tag="PK977_12615"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRF19008.1"
                     /translation="MFAVTILGNNSAVPAFDRHPTSQVVTLDGTNYLVDCGEGTQIQL
                     INYKIRRSKISHIFISHLHGDHYFGLIGLINTLSLLSHQQEMHLFGPAPLKEIIELQL
                     KVADTQLCFPLHIHHITEAATLVDNEKLTVKCFRTNHRIECYGFSFTQKKQPRKLNPE
                     KAKEFEIPSSFYERLTQGEDYTRKDGTVIKNELVTEPGTAGKRYAFCADTKYDESLIP
                     HIEGFDLIYHETTYLDNLRERAEARFHSTSKQAAMIAKKAGVKKLLIGHFSSKYDTLE
                     EFEKEAREVFPDTELALEGVAYTI"
     gene            complement(1233..>2184)
                     /locus_tag="PK977_12620"
     CDS             complement(1233..>2184)
                     /locus_tag="PK977_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF19009.1"
                     /translation="STDQKGNYFLILELPAGMYEYKITRGGWDKVECKRGGENIANRF
                     LKLESDTSITIEIEDWQDMSAMKQKKSTASSNVLIINTSFYIPQLKRTRRVWVYLPAD
                     YATSKQRYPVLYMHDGQNVFEDTTAYAGEWGVDEFLDTSKTKKCIVVAIDHGGDKRLN
                     EYSPYDMERFGKGEGKLYVDFLVKKLKKYIDRKFRTERGRGSTFVAGSSMGGLISMYA
                     LLKYPKKIGGAGIFSPAFWVAPAIYDDIRSKGSKVRGRVFFYAGKKEDETMVPLMVKA
                     FGELAAVSHAQLETVIRDEGKHNESTWRKEFPLFYQWLFR"
     assembly_gap    2185..2240
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2380..>2599)
                     /locus_tag="PK977_12625"
     CDS             complement(2380..>2599)
                     /locus_tag="PK977_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRF19010.1"
                     /translation="RLCLKGDYDKAKLLHFKYTDIIASMFAEGSPSGVKAYLIEMGLC
                     KNTFRLPVVAVSDKHLGKIKELMKQAED"
     assembly_gap    2600..2620
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2621..3266)
                     /gene="dapA"
                     /locus_tag="PK977_12630"
     CDS             complement(<2621..3266)
                     /gene="dapA"
                     /locus_tag="PK977_12630"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRF19011.1"
                     /translation="MSLREKFTGTGIAIVTPFHEDGKIDWASFGQLIEFWIKGEVEYL
                     VVLGTTGESATVHGDEKQEVFSFVAGQVAGRVPLVAGIGGNDTHEVLDGFKKFDLKSY
                     DAILSVSPYYNKPNQEGIFQHYKALDAATPLPIIMYNVPGRTGMNVTADTQLRIAHEC
                     KSIFATKEASGNFDQINQILKYKPADFMVISGDDPITLPMIALGAEGVISVVANA"
     gene            complement(3300..4271)
                     /locus_tag="PK977_12635"
     CDS             complement(3300..4271)
                     /locus_tag="PK977_12635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRF19012.1"
                     /translation="MPTDANRQFLDFEKPIKDLIDEIQTMKHRQEKTKIDFTDSIQKL
                     EKGILDKRKEITQNLTSWQRVQLSRHPDRPYTMKYIDKMTENFVELYGDRNVKDDKAM
                     VGGFASIDGETVMIIGQQKGINTKMRQMRNFGMANPEGYRKALRLMKLAEKFNKPIIT
                     LIDTPGAFPGLEAEERGQGEAIARNIFEMIRLKVPVICVIIGEGASGGALGIGVGDKV
                     FMMENTWYTVISPESCSSILWRSWDKKEVAAEQLKLTADKMLGFGLIDGIIPEPPGGA
                     HWDYNESGQILKNYLKPVLQELKQIPAEQRVNNRIEKFGKMGFWEEF"
     gene            4445..4518
                     /locus_tag="PK977_12640"
     tRNA            4445..4518
                     /locus_tag="PK977_12640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4479..4481,aa:Asp,seq:gtc)
     gene            4710..5462
                     /locus_tag="PK977_12645"
     CDS             4710..5462
                     /locus_tag="PK977_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRF19013.1"
                     /translation="MKKKFKSFYLLASSVIISILHLPFAFAKSASGSKLFFTPPSDSA
                     AKNAVDSMQFMPVMKSAYDSLHLNLRGLSLQAYEYAKMGFDKLLLEGKLVNDSIISII
                     DFSLPSNKKRMFVIDLKNYKVLHNTLVAHGKNTGTEWASSFSNQHSSHKSSPGFYLTR
                     ETYQGKHGLSLRLEGLEAGINDHAYERGIVVHGADYVSESLANARGFIGRSHGCPAVP
                     DQLSRPIINTIKEGTCLFIYHPSYVDKSVVLN"
     gene            5606..>5998
                     /locus_tag="PK977_12650"
     CDS             5606..>5998
                     /locus_tag="PK977_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF19014.1"
                     /translation="MLKIGVFGVGHLGKFHLNNWKEIAGTELVGFYDPNDEVAKEVAD
                     KYQLPRFLDADVLIDACDAIDVVAPTNYHFELCEKAIKKGKHVFVEKPLANTMDEARQ
                     LVKLAAESGIKFQVGHVERFNPAFLAIKD"
     assembly_gap    5999..6089
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6090..>6400
                     /locus_tag="PK977_12655"
     CDS             <6090..>6400
                     /locus_tag="PK977_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF19015.1"
                     /translation="MIHDIDIILSIVKSDVKSISASGVGVMTETPDIANVRIEFHNGC
                     VANLTSSRISMKKMRKIRLFQKDSYIGIDFLNKKTEIIKLKEPQDANVFAFDIETPKG
                     K"
BASE COUNT         1830 a         1375 c         1218 g         1809 t
ORIGIN      
        1 atgacgttaa aaacctggtg ctcattttaa aggaaacggt ttccattgat atagccgcag
       61 cagagaagct ggttaagata caacagcagt tttacgagaa cggggcatcc tttgtcatat
      121 gtgaattaca aaaacaggtg gaagatttcc tggatcagca ggaactgctg gaattgatga
      181 atgtaacccc tactgaaagt gaggcatggg atatagtgca gatggaagaa atagaacgag
      241 acctgatggg cgatgaataa ccatgcagca ttagtaccta aacattagtc taaagaaaaa
      301 ccattacaca cccaattact aaccagttca tgtttgcagt tacgatcctt ggcaacaatt
      361 ctgctgtacc ggcatttgac cgtcatccaa ccagccaggt ggtgacactc gacggcacca
      421 attacctcgt cgattgcggg gaaggtaccc agatacagct gatcaattat aaaattcgcc
      481 ggagtaagat ctcacatata ttcatttccc accttcatgg cgatcattat ttcggtttaa
      541 taggattaat aaatacactc agtttactga gccatcagca ggaaatgcac ctgtttggcc
      601 cggcgccttt gaaagagatc atcgaactgc aactgaaagt agctgatacg caattgtgct
      661 tccccttaca tatccaccac attacagaag cggccacact ggtagataat gagaaactta
      721 cggttaagtg ttttcgtacc aatcatcgta ttgaatgtta cggtttttct tttacccaaa
      781 agaaacagcc ccgcaaacta aacccggaaa aagcaaaaga atttgagata ccctcctctt
      841 tctatgaaag gttgacacag ggggaagatt atacccgaaa agacggtacg gttataaaaa
      901 atgaactagt aacagagccc ggtaccgccg gaaaacgata tgctttctgc gccgatacaa
      961 agtatgatga atccctgatt ccgcacattg agggatttga tcttatttat catgaaacaa
     1021 cctacctgga taaccttcgg gaaagagctg aagccagatt tcactccacc agcaaacagg
     1081 cagccatgat cgctaaaaaa gctggcgtaa aaaagttgct gatcgggcat ttcagcagca
     1141 agtatgatac actggaagaa tttgaaaaag aagcaagaga agtttttcct gatacagagc
     1201 ttgcgttaga aggagttgct tacactatct gatcaacgga atagccattg gtaaaacaaa
     1261 ggaaattcct ttcgccatgt tgattcatta tgttttccct catcccggat cacggtttcg
     1321 agctgtgcat gtgacactgc cgccagttca ccaaatgctt tcaccataag cggcaccatg
     1381 gtttcatctt ctttcttacc ggcataaaag aataccctgc ctcttacttt actccctttt
     1441 gatctgatat catcataaat agcgggagcc acccaaaaag ccggggagaa gattccggca
     1501 ccaccaattt tttttggata cttaagcaag gcatacatcg agatcagccc tcccatcgaa
     1561 ctgccggcaa caaaagttga ccccctgccc cgctctgtcc tgaattttcg gtctatatat
     1621 ttctttagtt ttttcaccaa aaagtcgaca tataatttgc cttctccttt gccaaatcgt
     1681 tccatatcat aaggtgaata ttcatttaac cgtttatcac ctccatgatc aatagcaaca
     1741 acaatgcact ttttggtttt gcttgtatcc aaaaattcat ctactcccca ttctccggca
     1801 taagcggtgg tgtcctcaaa cacattttgc ccgtcatgca tatacaaaac aggataacgt
     1861 tgctttgaag tggcataatc agcaggaaga tatacccaaa cccttcttgt tcgttttagc
     1921 tgagggatat aaaaagaagt attaataatg agaacatttg agctggctgt acttttcttt
     1981 tgtttcatgg cagacatgtc ttgccagtcc tctatttcta ttgtgatgga agtgtcactt
     2041 tccagcttca aaaaccggtt ggcaatattt tctccgcctc ttttgcattc cactttatcc
     2101 cagccacccc gggtgatctt atactcgtac atgccagcag gtaattcaag aatgagaaaa
     2161 tagtttcctt tctgatcagt actgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn gtaaatattg ccggtttgct tatttacccc tggtaatgac
     2281 tgaataaaaa agtgaacttt atgctgagct gaaagcaact gcaattgcaa taagaaaaag
     2341 gatattgtga aaatcctgta catcagcatt taacgatgat taatcttctg cctgtttcat
     2401 caactctttt atttttccta gatgtttgtc actgacagca actaccggaa ggcgaaaagt
     2461 atttttacaa agccccattt cgatcaaata agctttaaca ccgcttgggc ttccctcggc
     2521 aaacatagaa gcaataatat cagtgtattt aaaatgaagc agctttgctt tatcataatc
     2581 acctttcagg caaaggcgtn nnnnnnnnnn nnnnnnnnnn acgcattggc cactaccgaa
     2641 atcacacctt cagcacccag ggctatcatg ggtaaggtta tgggatcatc gccgctgata
     2701 accataaaat cagccggttt atatttcagt atctggttga tctggtcaaa attgccactt
     2761 gcttctttgg tcgcaaatat gcttttacat tcatgtgcaa ttcttaactg tgtatctgca
     2821 gtaacattca ttccggtacg ccccggtaca ttgtacataa tgatgggaag cggcgttgcg
     2881 gcgtccagtg ctttgtaatg ctgaaaaata ccttcctggt tgggcttatt gtaatatggt
     2941 gaaacagaaa gtatggcatc ataacttttc aggtcaaatt ttttgaaacc atcaagtacc
     3001 tcatgtgtat cattacctcc gatacccgcc accaatggta cacggccggc cacttgtccg
     3061 gccacaaaag aaaaaacctc ttgcttttca tccccatgta ccgtagcact ttccccggtg
     3121 gtacctaata ctaccaggta ctcaacttct cctttgatcc agaattcaat caattgacca
     3181 aaacttgccc agtcaatttt accatcctca tgaaaaggag taacaatggc aattcccgtt
     3241 ccggtgaact tttctctcag tgacattgtg taattattgt aaagtgaaaa aacgatcctt
     3301 tagaactctt cccaaaaacc catttttccg aatttttcga tcctgttgtt tactctttgt
     3361 tcggcaggta tttgctttaa ttcttgcaat accggtttca aataattctt gagtatttgg
     3421 cctgactcat tataatccca atgagcgccg cctggtggtt caggaatgat gccgtcaatt
     3481 aggccaaagc ccaacatttt gtcagctgtt aactttagtt gttcagctgc cacttctttt
     3541 ttatcccagc tacgccaaag aatggatgag cagctttccg ggctgattac agtataccat
     3601 gtattttcca tcataaatac cttatccccc acaccaatac ctaaagctcc gccactggcc
     3661 ccttccccta ttatgacgca aataaccggt actttcagac ggatcatttc aaaaatattg
     3721 cgggcaatag cttcaccctg gcctctttct tcagcctcca ggccgggaaa agcacccggt
     3781 gtatcaatta atgtaatgat aggcttattg aatttctcag ccagcttcat caggcgcagc
     3841 gccttcctgt atccctccgg attggccatc ccaaaattcc gcatttgcct cattttggta
     3901 ttgattcctt tttgctgacc aatgatcatc accgtttcac catcaattga tgcaaaacct
     3961 cctaccatgg ctttatcatc ttttacattc cggtcgccat ataactccac gaagttttcc
     4021 gtcatcttat cgatatactt catcgtatat ggcctgtccg ggtgacggct tagttgtacc
     4081 cgttgccagc tggtgaggtt ttgcgtaatt tcttttcgct tatctaaaat ccctttttca
     4141 agcttttgta tagaatctgt aaaatcaatt ttggttttct cctggcgatg cttcatcgtc
     4201 tggatctcat caatcaggtc cttgatgggt ttttcgaagt ctaaaaactg tctgttagca
     4261 tcggttggca taggctaaaa atgtggcggc taaattaagg attaaataat aataaggtga
     4321 tgaggtcaaa atgctgaatt acaagtggat aattaatcta aaaacttttg gctgaaaata
     4381 attacaggac tatctttgcc gtcctgaaaa atgaaaccgg taacggttga ttttgaaagg
     4441 taacggatcg gtagttcagt tggttagaat gccgccctgt cacggcggag gtcgcgggtt
     4501 cgagtcccgt ccggtccgca ataattgacg agtgatttga ttcaaatcaa ctcaaactga
     4561 aacaaaaact gcccggagat tgatctcggg catttttatt ggtagttagc aaggttttaa
     4621 catcccaggc aggcattgca taaaagaaga acccggtttc ctcgtttaat gtggtaaggt
     4681 ttttattggt taacggttaa atagtagcaa tgaagaaaaa gttcaaaagc ttttatctgc
     4741 tggcatcgtc tgtaattatc agtatcctgc atctcccttt tgcttttgca aaatcagcca
     4801 gcggttccaa attatttttt acaccccctt ccgactctgc cgcaaagaat gctgtggatt
     4861 caatgcagtt tatgcctgta atgaaatcag catacgacag tctgcatctg aacctacgtg
     4921 gactcagcct gcaggcatac gagtatgcta aaatgggttt cgacaaatta ttactggagg
     4981 gtaaactggt gaatgattct atcatttcca ttattgattt cagtcttccc agtaataaaa
     5041 aaaggatgtt tgttattgac ctgaaaaatt acaaggtcct acacaacaca ttagtggcac
     5101 acggtaaaaa tacaggtaca gaatgggcta gttcgttttc caaccagcac tcctcacaca
     5161 aaagcagtcc cggtttttac ctgaccagag aaacatacca gggaaaacat gggctttctc
     5221 tcaggttaga aggacttgaa gccggcatta atgaccacgc ctatgaaagg ggaattgtag
     5281 tgcatggcgc tgactatgtt tcagaaagtc ttgccaatgc aagaggtttc attggccgca
     5341 gtcatggttg tcccgctgtt ccagatcagt tgtctagacc aatcataaat acaatcaaag
     5401 agggtacctg ccttttcatc tatcaccctt cctatgtaga taaatcagta gttcttaatt
     5461 aaactttcta aataatacaa cagcctcccg gtataaaagg acaagtttcc agtaaccggg
     5521 aggctttctt tttgcctgca attttgtttg tatcccggaa ttaactgcct gaccaatctt
     5581 tatctttgag gattaattaa ctcttatgct caaaattggt gtttttggag taggccacct
     5641 cggtaagttt cacttaaaca actggaaaga aattgcaggt acagaactgg ttggctttta
     5701 tgacccaaac gatgaagtag caaaagaagt ggcagacaaa taccagttac cccgtttctt
     5761 agatgcagat gtactgattg atgcctgtga tgccattgac gtggtagccc ccaccaatta
     5821 tcattttgaa ttgtgcgaaa aggcaatcaa aaaaggcaag catgtatttg tggagaaacc
     5881 attggccaac acaatggatg aggcaagaca actggtgaaa ctggctgcag aatcaggtat
     5941 aaaattccag gtaggacatg tagaaagatt caatcctgca tttttagcca ttaaggatnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng atgatacatg atattgatat cattttaagt
     6121 attgtaaaaa gtgatgtaaa aagtatttcg gccagcggtg taggtgttat gacagagacc
     6181 ccggatatcg ccaacgtacg cattgaattt cataatggtt gtgtagccaa ccttacaagc
     6241 agcaggattt caatgaaaaa aatgcgaaag atacgcctgt tccaaaaaga ctcttatata
     6301 ggaattgact tcctcaacaa gaaaaccgaa atcatcaaat taaaagaacc acaggatgct
     6361 aatgtttttg cgtttgacat cgaaacacca aaaggcaaaa
//
LOCUS       DAORLS010000173         6345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6646_length_6345_cov_2.422893, whole genome shotgun sequence.
ACCESSION   DAORLS010000173 DAORLS010000000
VERSION     DAORLS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_6345_cov_2.422893"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2185
                     /locus_tag="PK977_12660"
     CDS             <1..2185
                     /locus_tag="PK977_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF19016.1"
                     /translation="IPFSIKSSLLNTNQHFTSLWMLEDNSFLAGTYFNGIYRISSGGT
                     EIEHISTENGMPNNTVRCLFADNFSNAWAGLDNGLAFFTYNNAVRHINPVSFNNGAGY
                     DAKVLNGDLYFALSTGLQWISAKSASDLSAITYEPKTIIGGLTWNLSVINNQLMAGRD
                     DGLYTVNNYLAKPISQTTGFWGCRPLLQTNPLKIIAGNYLGVHFFEMDNGNLIDAGSL
                     KGFTESSRYIETDEGSIWISHPYRGIYRINLVDRSVRQFAQKEGLPANLDNHVFKIKG
                     KIVFATSRGIYEYNKTTDKIIKSSAYADLFGELPIRYMKEDEKGNIWFVQEKMTGVAD
                     YSTGQPVIHYIPELKNKILSGFENILPYDSRNILVGGEAGFYHINYEKYQEKIKPFAA
                     YVTQIKTTGASDSILFGGYIFDSTGLQKTITIPYKLNSLLFSYAASAYGQHNLSEFSY
                     YLQGFDKGWSSWSDNIQKEYTNLPAGTYTFNIKSRQSPSHESSVYQITFTINPPWYQT
                     MWAYALYFILIVGSLYALLRYQSRRHRTKQEARRLADQLKFEEEQKQMAYQHQLELAK
                     SEKELIHLQNEKLEAEIKHKNAELASATMNLVQKKEFILKLKSELQQLQKNAKVGDDN
                     PELKKLLKVLSEEEKLDEEWNSFSQHFNSVHGDFLTILKSKFPSLKPHELRLCAYLRM
                     NLSSKEIAPLMSISIRGVEISRYRLRKKLALPTEVNLVQFLMDLK"
     gene            2297..3346
                     /locus_tag="PK977_12665"
     CDS             2297..3346
                     /locus_tag="PK977_12665"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRF19017.1"
                     /translation="MFKSKIAGIGMYVPERVVTNNDLLKYMDTSDEWIQERTGIKERR
                     YAHRTEETTTTMGVEAAKIAIERAGITPQDIDFIVFATLSPDYYFPGCGVLLQRVMKM
                     KEIGALDVRNQCSGFVYALSVADQFIKTGMYKNILVVGAEKHSFGLDFSTRGRNVSVI
                     FGDGAGAVVLQPTNEENRGILSTHLHSDGESAEILAMYNPGTHANHWKEEKYADFDEA
                     EIAQMFFSHAMIDKAQVFPYMDGPAVFKKAIVKFPEVIIESLQANGLQTSDLKLLIPH
                     QANLRISQFVQQKLQLRDDQVYNNIQKYGNTTAASVPIALCEAWENGKVKEGDLICLA
                     AFGSGFTWASALLRW"
     gene            3393..3815
                     /locus_tag="PK977_12670"
     CDS             3393..3815
                     /locus_tag="PK977_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19018.1"
                     /translation="MKKLIFTVLSLAFFTMVQSQESWTIGYGKKTVLKKVTEDRVKNS
                     FTLSKSSVSGNSKLTIQLHAVDTANNITLMANLDNGNTVKEWEYSDKTLSIPAGELKT
                     LFGTESKLHFYYRSLPKDPEKAAVVRIRPVHVCTVTLK"
     gene            3860..4762
                     /locus_tag="PK977_12675"
     CDS             3860..4762
                     /locus_tag="PK977_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRF19019.1"
                     /translation="MSRHILYIINPISGTRKKKDLQAFIEKETTERNIPFSIFPSVAN
                     GDYTFLHGIIKEKQVTDIVIAGGDGTVSQVVNSLMDFDLNFGIIPCGSGNGLAYAAKI
                     SKDPVNALQTIFNGTAAATDGFFINGHFACMLCGLGFDAKVAHDFAQQLKRGLSTYIK
                     QVVKNFFAAHTYSFEITVNKEIFSIEAYFISIANSNQFGNNFTIAPKASLSDGLLDIV
                     IVTSQSKLSVLLQTLRQVSGRNKLQPETAVDKNKAVIYFQTDRIIITNISNAPLHIDG
                     EPAETPEKINIQVKPGCFRLIQPL"
     gene            complement(4746..>5199)
                     /locus_tag="PK977_12680"
     CDS             complement(4746..>5199)
                     /locus_tag="PK977_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19020.1"
                     /translation="LRDNYGQETTLRFLSHFDEWIKQNTSGVLVLQIRDRKAGGWEMP
                     YLSDDITDHVTKPFLLLQHNWFKMMEFFQNDMLNYYVTKSGIPVHKIAFQYAADKEEN
                     KAALNFHLTKREEKDIISSIHSTINRQSFQQLISLLSKKPAPDTTRVE"
     assembly_gap    5200..5240
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5241..>6345)
                     /locus_tag="PK977_12685"
     CDS             complement(<5241..>6345)
                     /locus_tag="PK977_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19021.1"
                     /translation="SHYTNDFIDSIFKRHHFAAIISVFAAFMFLVIIGFLLDNPFFQL
                     PAAASITIFFSILIGVAAAITYFLQSWSIPYLIGLVLLLNIFYKLDWIDPRNKAYGID
                     YWNKKERPAYSREGLLALCSPENIASDKKNMISVLDAWKKKQDSEKPLLIIVNTSGGG
                     HRSATFTMSVLQQLDSITGGSFMKKTFLINGASGGMIGAAYFRELYLRKQQGINIHLQ
                     DKKYVDDISGDLLNPLFTSFFARDLIAPAQKFSVGPYRYVKDRGYSFEEKLNDNTRSF
                     LDKQLKDYVADEKAAKIPLMFFSSVVTRDSRKMIISTQPVRFMMQAPMDTTNIPRMDP
                     DAVDFVSFFSKQDPYNLRMLSELRMNATFPIVLH"
BASE COUNT         1990 a         1322 c         1285 g         1707 t
ORIGIN      
        1 gataccgttc agtattaaat cttcattact gaacaccaat cagcacttca cttcactctg
       61 gatgctggag gataacagtt ttttggcggg tacttacttc aatggcatat accgtatatc
      121 aagcggaggt acagaaattg aacatatctc caccgaaaac ggaatgccca ataatacggt
      181 aagatgcctt tttgctgata atttcagtaa tgcatgggct ggcctggata atgggctggc
      241 cttttttacg tacaacaatg ccgtcaggca tataaacccg gtttcgttca ataacggcgc
      301 tggctatgat gccaaggttt taaacggaga tttatatttt gccctttcca caggactgca
      361 gtggatttct gctaaatccg ccagcgacct tagcgccatt acttatgaac ccaaaacaat
      421 aattggcgga ctgacatgga acctatctgt tatcaataat caattgatgg ccggacggga
      481 tgatggcctt tatactgtta acaattacct cgcaaaacca atttcacaaa caaccggctt
      541 ttggggctgc cgccctttgt tgcaaacaaa tcctcttaaa attattgcag gaaattacct
      601 gggtgttcat ttttttgaaa tggacaatgg aaatcttatt gatgccggat cattgaaagg
      661 atttaccgaa tcaagccgtt atattgaaac tgatgaaggc agcatctgga tatcacatcc
      721 atacaggggt atttacagaa taaaccttgt tgaccggtct gtacgacaat ttgcacaaaa
      781 agaaggactg ccggcgaatc tcgataacca tgtttttaaa ataaaaggaa aaattgtatt
      841 cgctacttcc cgtggtatct atgaatacaa taaaaccaca gataaaatca ttaaatcatc
      901 agcttatgca gacctgttcg gcgagctacc catacgttac atgaaggaag atgaaaaagg
      961 gaatatctgg tttgtacagg agaaaatgac cggcgtggcc gattacagta ccgggcagcc
     1021 ggttattcat tatataccgg agctgaaaaa caaaattcta agtggctttg aaaatatttt
     1081 accctatgat agccgcaata tactggtggg tggcgaggca ggtttctatc acatcaatta
     1141 tgaaaaatac caggaaaaaa taaagccctt tgctgcctac gttacacaaa taaaaacaac
     1201 cggggccagc gacagcattt tgtttggtgg atatattttt gacagcacag gtttacaaaa
     1261 aacaattacc attccttata aacttaattc acttctcttc tcttatgcgg cctcagccta
     1321 tggccagcac aatctttcgg aatttagtta ttacctgcag ggttttgata agggctggag
     1381 cagctggtcc gacaatatac agaaagaata caccaatctt ccggccggta cttacacatt
     1441 taacatcaag tcaagacaaa gcccctcaca tgaatcttca gtttaccaga ttacatttac
     1501 catcaatcca ccctggtacc aaacaatgtg ggcttatgca ctctacttta tattgatagt
     1561 tggctctttg tatgcattac tgagatatca gtcacgaagg catagaacaa aacaagaggc
     1621 aagaagattg gccgatcagc taaaattcga agaagaacaa aaacaaatgg cttaccagca
     1681 ccagctggaa ctggcaaaat cagaaaaaga actcatccat ttgcaaaatg aaaaactgga
     1741 agcagaaatt aaacataaaa atgcagaact ggccagcgcc accatgaacc tggtacagaa
     1801 aaaagagttt atcctgaaat taaaatcaga attgcagcaa ctgcaaaaaa atgccaaagt
     1861 tggagacgat aatcctgaac tgaaaaagtt gcttaaagtc ttatccgaag aggaaaaact
     1921 ggatgaagaa tggaacagct tttcccagca ttttaatagt gtacacggag attttttaac
     1981 gattttaaaa agtaaattcc cttccttaaa accacatgag ctgcggctct gtgcctatct
     2041 gcgtatgaac ctgagcagca aagaaattgc cccgcttatg agtatctcta tccggggagt
     2101 agagataagc aggtacaggc tcagaaaaaa actggcattg cccacagagg ttaaccttgt
     2161 acaattctta atggatttaa aataaacaaa cactaataaa acattcgtgg ccgaagtatc
     2221 atctatttac aactgtttcg cttgaaacag tttcatttcc ttcacatttc ctaacttccc
     2281 cgtttaaatg cttgctatgt tcaaaagtaa aattgccggt atcggcatgt atgtacctga
     2341 aagagtagta accaataacg acctgctgaa gtatatggac accagtgatg aatggataca
     2401 ggaacgtacc ggtatcaaag aacgccgata tgcccaccgt actgaagaaa ccaccaccac
     2461 gatgggagtg gaagcggcaa aaatcgccat agaaagagca ggtatcaccc cgcaggatat
     2521 agacttcatt gtatttgcaa cactcagtcc tgactattat ttcccgggtt gtggtgtatt
     2581 gctgcaacgg gtcatgaaaa tgaaagaaat cggtgcattg gatgtacgaa atcagtgcag
     2641 cggctttgtg tatgccctta gtgtggccga ccagttcatc aaaacaggta tgtacaaaaa
     2701 catcctggta gtgggagcag aaaagcattc ttttggtctt gatttcagta cgagggggag
     2761 aaatgtatca gtgatatttg gcgatggtgc aggtgccgta gtattacaac ccactaatga
     2821 ggaaaacaga ggtatactaa gcactcattt acatagcgat ggtgaaagtg ccgagatact
     2881 ggctatgtac aacccgggta cacatgccaa ccactggaaa gaagaaaaat atgcagactt
     2941 tgatgaggct gagattgccc agatgttttt cagtcatgcc atgatagata aggcgcaggt
     3001 atttccttat atggatggcc cggccgtttt taaaaaggcc attgttaaat tcccggaggt
     3061 gatcattgaa tcgttgcaag ctaatgggct tcagacttca gaccttaaat tactgatccc
     3121 ccaccaggcc aacctgcgca tctcacagtt tgtacaacaa aaattgcagt tgcgtgatga
     3181 ccaggtatac aacaacattc agaagtatgg caacactacc gctgcttccg tgcccattgc
     3241 actttgcgaa gcctgggaaa acggcaaggt gaaagagggt gatcttattt gcctcgctgc
     3301 ctttggaagt ggttttacct gggccagcgc tttgctaagg tggtagcata caagctatgc
     3361 aacgcagcat caaacagcat catctttata gcatgaaaaa attaatattt acagtactat
     3421 ccttagcttt ttttaccatg gtacaatcac aggaaagctg gacaatcgga tatggtaaaa
     3481 aaactgtcct gaaaaaagta acagaagacc gggtaaagaa ttctttcacc ttaagtaaat
     3541 cttctgtttc cggcaattca aaactgacaa tacaattaca tgcggttgat acagcaaaca
     3601 atattaccct gatggctaat cttgataatg gcaacactgt aaaagaatgg gaatacagcg
     3661 ataaaacttt aagcatcccg gccggcgaac ttaaaactct atttggcact gaaagcaaac
     3721 ttcattttta ttatcgctcc ctgccaaaag atccggaaaa ggcagcagtt gtacgaatac
     3781 ggcctgtaca tgtttgtact gttacgctaa agtgatggaa tatctttttt agcgccggct
     3841 cttcttacat ttgctttgca tgagccgaca cattctttat attattaatc caatttcagg
     3901 cacccggaaa aaaaaagacc tgcaggcctt tattgaaaaa gaaaccaccg aaagaaacat
     3961 tccctttagc atatttccat ctgttgcgaa tggcgattat acatttctgc atggtatcat
     4021 taaggaaaaa caagttacgg atatagtaat cgccggtggt gatggaactg tcagccaggt
     4081 agtcaatagc ctgatggact ttgatctgaa ttttggtatc atcccctgcg gctccggtaa
     4141 cggacttgcc tatgccgcaa aaatttcaaa agatcccgtt aatgcactac aaacaatatt
     4201 caacggcacc gctgcagcta ctgatggctt tttcatcaac gggcatttcg cctgcatgct
     4261 ttgcggtttg ggttttgatg ccaaagtagc ccacgatttt gctcagcaac tgaaaagggg
     4321 tttatctact tacattaaac aggttgtcaa aaacttcttt gctgcacata cttattcttt
     4381 tgaaataacc gtaaacaaag aaattttttc aattgaggcc tacttcatca gtattgccaa
     4441 tagcaaccag tttggaaaca actttaccat tgcacccaaa gccagtctta gcgacgggtt
     4501 gctggatatt gtgattgtga ccagtcaaag caaacttagt gtgctgctac aaacccttag
     4561 gcaggttagc ggaagaaata agctgcaacc ggaaactgca gtggataaaa acaaagcagt
     4621 gatctatttt caaactgaca ggataattat aacaaacata tcaaatgctc ccctgcatat
     4681 tgacggagaa cctgcagaaa cacctgagaa aattaatata caggtaaaac ccggctgctt
     4741 caggcttatt caacccttgt agtatctggt gcaggtttct ttgacaataa actaataagc
     4801 tgctgaaaac tttgcctgtt aatcgttgaa tggatagatg aaataatatc tttttcttcc
     4861 cttttagtca aatgaaaatt gagtgcagct ttattctctt ctttatcagc cgcatactga
     4921 aaagcgattt tatgaacggg aatgccactt tttgtaacat aatagttcag catatcattc
     4981 tgaaagaatt ccatcatctt aaaccagttg tgctgcaata ataaaaatgg cttggtgaca
     5041 tggtctgtaa tatcatctga gagataaggc atttcccatc cgcctgcttt gcggtccctg
     5101 atctgcagca ccagcacacc gctggtattt tgttttatcc attcgtcgaa atgcgaaaga
     5161 aaacgaaggg tggtttcctg accgtaatta tctctcaacn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn gtgcaacacg attgggaaag ttgcattcat gcggagttca
     5281 ctcagcatcc gaagattata ggggtcctgt ttggaaaaga aagaaacaaa atcaaccgca
     5341 tcgggatcca tgcggggaat attggtggta tccatcggcg cctgcatcat aaatcgcact
     5401 ggctgcgtgg agatgatcat cttgcggcta tctcttgtca ccaccgagct gaaaaacatc
     5461 aacggaattt tagccgcttt ctcatcagca acataatcct ttaactgctt atctaaaaaa
     5521 cttctcgtat tatcattgag cttttcttca aatgaataac ccctgtcttt tacatagcga
     5581 taaggtccta cagaaaattt ttgcgccggc gcaatcaggt ccctcgcaaa aaaggaggtg
     5641 aataaagggt ttagcaaatc acccgaaatg tcgtctacat attttttatc ctgcaaatga
     5701 atatttatcc cttgctgctt tcgcaggtat aattccctga aataagcagc gccgatcatg
     5761 cctcccgaag caccgttaat aagaaatgtc tttttcatga agctcccacc ggtgatactg
     5821 tctaactgtt gcagtacact catggtaaac gtggcactgc ggtgaccacc tccgctggtg
     5881 tttacaatga tcagcaatgg tttttcgcta tcctgtttct tcttccaggc atccagcact
     5941 gaaatcatat tctttttgtc tgatgctata ttttcagggc tgcataatgc gagaagacct
     6001 tctcttgaat aagcaggcct ttctttttta ttccagtaat ctatgccgta ggctttattg
     6061 cggggatcga tccagtctaa cttgtaaaaa atattcagaa gcaaaaccag gccgataaga
     6121 tatggtatgc tccagctttg taaaaaataa gtgatggctg cagcaacacc gatcagtata
     6181 gagaaaaaga tagtgatgct ggcagcagca ggtaactgga aaaaaggatt gtcaagcagg
     6241 aaaccaataa tgaccaggaa cataaaagca gcaaacacag atatgatggc cgcaaagtga
     6301 tgccgcttaa aaatagaatc aataaaatcg ttggtatagt gcgaa
//
LOCUS       DAORLS010000174         6301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6710_length_6301_cov_1.767371, whole genome shotgun sequence.
ACCESSION   DAORLS010000174 DAORLS010000000
VERSION     DAORLS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6710_length_6301_cov_1.767371"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK977_12690"
     CDS             complement(<1..213)
                     /locus_tag="PK977_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19022.1"
                     /translation="MKKIILFISAIFFTISLPGQTKTFRGAWFDIKYPSAFKVNPSLK
                     SATNTNGYESVFFISPDRLVEFYVFSP"
     gene            complement(340..1401)
                     /locus_tag="PK977_12695"
     CDS             complement(340..1401)
                     /locus_tag="PK977_12695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF19023.1"
                     /translation="MKYIFYLTVLLTLFSCQKDQQPAPAEETMYYPPAGSSTWETKSV
                     SSLGWNTAAVQPLKDYLQQKNTKSFMILVNGRIVMEEYFNGHTSNTSWQWNSAGKTLT
                     GTMTGIAQQEGLLQINNKVSDYIGTGWTSAPINKENLITNRHLLTMTSGLNDANNLVI
                     PSNLTYIADAGTRWSYHNVFQKLMDVVAAASSQSYDAYFNAKLRDRIGMDGFWNNGII
                     FKIYHSTTRSMARFGLLALNKGKWNNEQIINQSFFEESINTSQSINPSYGYFWWLNGK
                     SSAMLPGSQTVYPGTLIPNAPAEMYAAMGAEDQRIYVVPSKKMVVIRMGNASDPQNPV
                     FALSGFDNALWEKINAVIN"
     gene            complement(1499..1777)
                     /locus_tag="PK977_12700"
     CDS             complement(1499..1777)
                     /locus_tag="PK977_12700"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRF19024.1"
                     /translation="MEKEIVWTSVAQNDFWEIAEYLKAEWPEKVLNQFQKALALKTAL
                     LKKLPNLGFKSHKYSQFRKTLVTRHYMVIYSVSKEHIIIHRIKHTRMR"
     gene            complement(1762..1989)
                     /locus_tag="PK977_12705"
     CDS             complement(1762..1989)
                     /locus_tag="PK977_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19025.1"
                     /translation="MNETIKQELHQLVDQCNNEQILEDVRELLQSNKDWWDELSETDQ
                     QLVKESEAEYNKGNFISHEELMQRFEQWKKK"
     assembly_gap    2234..2337
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2338..>2653)
                     /locus_tag="PK977_12710"
     CDS             complement(<2338..>2653)
                     /locus_tag="PK977_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRF19026.1"
                     /translation="NGSSGGVGHFAVQVAKAYGAKVTAVCSSKNMEFVKSLGADEVLA
                     YDKENIHRHNGKYDLVVDTNGNLTFNDYTRMGKRGVLTGFTTMGHMISVLLKKAFSKF
                     PLV"
     assembly_gap    2654..2812
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2813..3087)
                     /locus_tag="PK977_12715"
     CDS             complement(<2813..3087)
                     /locus_tag="PK977_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRF19027.1"
                     /translation="MYGGPEVLNLEVVEKPELKDDHILVKVMANSANPVDWHIIRGKP
                     FFARFTFGLFKPKDKIPGADFAGIVEAVGKNITRFKTGDRVFGESLKG"
     assembly_gap    3092..3110
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3131..3688)
                     /locus_tag="PK977_12720"
     CDS             complement(3131..3688)
                     /locus_tag="PK977_12720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRF19028.1"
                     /translation="MEKLILHTGRLLIRNLNLSDLSNFHAYRSNPEVTKYQGFDVFTM
                     EQAESFIISQKDKTFGNAGEWVQYGIENKTTGKLIGDCAIKLDQYDTRLAEIGITIAP
                     EEQKKGYAKEVLNAILNFLFSIEDFHRVTETVDAENTASIQLLKSIGFRQEGHFIENI
                     FFKGKWGSEYQFAMLKREWIQLNLS"
     assembly_gap    3731..3793
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3794..4600)
                     /locus_tag="PK977_12725"
     CDS             complement(<3794..4600)
                     /locus_tag="PK977_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF19029.1"
                     /translation="MKQEKKYDIIIVGGSYAGLAAAMALGRALRKVLVIDSGNPCNQQ
                     TPHSHNFLTNDGKTPKEIAGIARQQVQQYKTVDFLNGFVSSGRKTETGFVVETETKET
                     YAAKKIVFATGIKDSMPAIPGFAECWGISVLHCPYCHGYEVRHQKTGILANGNDAVEL
                     ATLISNWTDDLTVYTNGKSTLSEEQVQKLQQQNIHINQNEIEKLEHNKGYVERILFKD
                     GAHATVKAIYARLPFVQHSSVPQMLGCELTPEGYIKIDPAQRTTVPGIFAC"
     assembly_gap    4679..4817
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5034..>5381)
                     /locus_tag="PK977_12730"
     CDS             complement(5034..>5381)
                     /locus_tag="PK977_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF19030.1"
                     /translation="EITTTQSTYTQDRFEELLRLLETNISHMKNVVQYAGLLNLSPYQ
                     LNAIAKAAVGKTVSALINEQIILEAKRYLLATSGQVKDIADQLGYEDVSYFIRFFKKQ
                     TGHSPEAFRKNFK"
     assembly_gap    5382..5609
                     /estimated_length=228
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5610..>5909)
                     /locus_tag="PK977_12735"
     CDS             complement(<5610..>5909)
                     /locus_tag="PK977_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRF19031.1"
                     /translation="KPIPVRHITSTPNEQDITGRFSFRKLEELMNGKNLVHDLHKHNF
                     YFVLAIQKGKGLHEIDFVNYQVKDNTVFMLRPGQVHRLELKAGATGFLLEFDLAF"
     assembly_gap    5910..5977
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6114..>6301)
                     /locus_tag="PK977_12740"
     CDS             complement(6114..>6301)
                     /locus_tag="PK977_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19032.1"
                     /translation="ITDNPRTSINSLTNFKKTTDLIRNSRIFSTMINRDEALKELEEI
                     ERDLLKEGKENYLIEDF"
BASE COUNT         1426 a         1114 c         1171 g         1810 t
ORIGIN      
        1 gggagagaat acataaaact caaccaggcg atcaggcgaa ataaaaaaga cgctttcata
       61 gccattggta tttgtcgctg attttaaaga tgggtttact ttaaatgcag aagggtactt
      121 tatatcaaac catgctcctc taaatgtttt ggtttgtccc ggcaggctga ttgtaaaaaa
      181 tattgctgat atgaagagga ttatcttttt cataaactac aatttggaga gtgaggtaat
      241 atacatcctc aaagagctgt ctttctttat ataaagatac atccatgcct ccgggttgag
      301 aaagcgaaag caacttagtt ctttaatccc tggaagtatt taattaatta ccgcatttat
      361 tttttcccat agtgcattat caaagccgga tagggcaaaa acagggtttt gcggatcgga
      421 ggcattgccc atgcggatga ctaccatttt tttggaaggc actacgtaga tccgctggtc
      481 ttcggcaccc atagcggcat acatttcagc aggtgcattg ggaatgagtg taccgggata
      541 aacagtttga ctgccgggaa gcatagcgct gcttttacca ttcagccacc agaaatagcc
      601 ataggatgga ttgatggact gcgaagtatt gatactttct tcaaaaaagg actggttgat
      661 gatctgttca ttgttccatt tgcctttatt gagtgccagt aagccgaaac gggccatgct
      721 gcgggtggtg ctgtggtaaa ttttaaaaat gataccgttg ttccaaaagc catccatgcc
      781 aatccggtct ctcagtttgg cgttgaaata agcatcataa ctctgtgatg aggcggcggc
      841 cactacatcc atcagttttt gaaatacatt atggtaagac cagcgggtgc ccgcatcagc
      901 tatataagta agattggagg gaataacgag attatttgca tcgttaagcc cggatgtcat
      961 agtaagcagg tgccggttag tgatcagatt ttctttattg atgggtgcac tggtccagcc
     1021 ggtgccgata tagtcggata ctttattatt gatctgcaac aggccttcct gctgcgcaat
     1081 acctgtcatg gttcctgtaa gtgttttacc ggcactgttc cattgccagc tggtattgct
     1141 ggtgtgcccg ttgaaatatt cctccatcac aattcgtccg ttcaccagta tcataaatga
     1201 tttggtgttt ttctgctgca ggtaatcttt cagcggctgt acggcggcag tgttccagcc
     1261 gagtgaagag acggattttg tttcccaggt gctgctgccg gcaggagggt agtacatcgt
     1321 ttcttcggca ggtgccggtt gttggtcttt ttgacaagaa aaaagagtaa gcagtacggt
     1381 taggtaaaaa atatatttca tagttggaag attagcggcg ggttggatgg gttattgggt
     1441 taaaggttta atgggtcttt gatcctcttt atatgtttga atcaatctaa gtattgcttt
     1501 aacgcatccg ggtatgtttt atcctgtgta taatgatgtg ttctttcgaa acggagtaaa
     1561 taaccatgta atggcgggtg accagtgttt ttctaaactg cgagtattta tgacttttaa
     1621 aaccaagatt aggcagtttt ttcaaaaggg ctgtctttaa ggctaatgct ttttgaaact
     1681 ggttaagtac tttttctggc cattcagctt tgaggtactc agctatttcc cagaaatcat
     1741 tttgggcaac ggatgtccag actatttctt tttccattgc tcaaaacgtt gcattaattc
     1801 ttcatgactg ataaagttgc ctttattgta ttcggcttct gactctttaa caagttgctg
     1861 gtcggtttcg gataattcat cccaccagtc tttatttgat tgcagcaatt cccttacatc
     1921 ttccaatatc tgttcattgt tacattggtc taccagttga tgaagctctt gttttatggt
     1981 ctcgttcata tcaattcgtt tgattataaa attagcaaaa taatttctcc taattaagcc
     2041 tgaattggtt ttgagaagct caactgatgg agatagttat aagaatagtt aaaggtttta
     2101 ttgctcatta aaactattaa tgaacgatag gggagtataa ccaatcactc agcggcatcc
     2161 tcccaaacca tcactacttt tcctcttgtt cgcatggcct cgatataacc gacagcctcc
     2221 gggatttgtt tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa
     2341 cgagcgggaa tttgctaaaa gcttttttta ataaaacgga aatcatgtgt cccatggttg
     2401 tgaatcctgt caatacaccc cgctttccca ttcgagtata atcgttaaac gtaagattgc
     2461 cattggtatc tacaacaaga tcatattttc cattatgccg gtgaatgttt tctttatcat
     2521 aggcaagtac ctcatcagcg cctaatgatt ttacgaactc catgtttttg gaagaacaaa
     2581 ctgctgttac tttagcgcca tatgctttag ctacctgtac tgcaaaatga ccaacaccgc
     2641 ctgaagaacc attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccttttaa
     2821 gctttcacca aatacccggt caccggtttt gaaccgggtt atatttttcc caactgcttc
     2881 tacaatacct gcaaaatcgg ccccgggtat tttgtctttg ggtttgaata acccgaaagt
     2941 aaacctggcg aaaaagggtt tgccccgaat gatatgccag tcaacgggat tggccgagtt
     3001 ggccatcact ttcaccagta tatgatcatc ttttagttcc ggtttttcca ctacttccag
     3061 attcagtact tccgggccgc cgtatattgt tnnnnnnnnn nnnnnnnnnn ttcattttgt
     3121 tttgttacgg ttaagaaagg tttaattgta tccattccct tttcagcatg gcaaactggt
     3181 attcgcttcc ccatttgccc ttgaaaaaaa tattttctat aaaatgtcct tcctgtctga
     3241 aaccaatgct tttcagcaat tgaatggaag cagtgttttc tgcatcaacg gtttctgtta
     3301 ccctgtgaaa atcttcaatt gaaaacagga aattcagaat tgcatttaat acttctttgg
     3361 catatccctt tttttgctct tccggtgcaa tggtaatgcc gatctcagca agcctcgtat
     3421 catattgatc aagctttatt gcacaatcgc caataagttt accggttgtt ttgttttcaa
     3481 tgccatactg cacccattcg ccggcatttc caaaagtttt atctttttga gaaatgataa
     3541 atgattccgc ctgctccatt gtaaacacat cgaagccctg gtatttggtc acttcgggat
     3601 tggaacgata agcatgaaaa ttgctcaggt ctgataagtt caggttcctg atcagcaaac
     3661 ggccggtatg taaaatcaat ttttccattg gcagatttaa aaagtttctt caatcaattc
     3721 tttattcagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnngcaggcg aagatacccg gaacggttgt tctttgtgcc ggatcaattt
     3841 ttatataacc ttctggcgtc aactcgcagc cgagcatttg cggtaccgat gaatgctgta
     3901 caaaaggaag ccttgcataa atggctttta cggttgcatg ggccccgtct ttaaacagga
     3961 ttctctccac atagcctttg ttatgttcca gcttttcaat ttcgttttga ttgatatgga
     4021 tattttgctg ctgaagtttt tgtacctgtt cctcagaaag ggttgatttt ccgttggtgt
     4081 aaaccgtcag gtcatctgtc cagttggaaa taagtgtggc cagttccact gcatcgttgc
     4141 cattggccag tattccggtc ttttgatgac gtacttcata accatgacag taaggacaat
     4201 gcagcacact gatgccccag cattcggcaa agccgggtat ggcgggcata ctatctttaa
     4261 tgccggtggc aaacactatt tttttagcag cgtatgtttc ttttgtttct gtctccacga
     4321 caaagccggt ctccgttttt ctgccagagc taacgaaccc gttcagaaaa tcaaccgttt
     4381 tatattgctg cacctgctgg cgggcaatac cggcaatttc ctttggcgtt ttaccatcat
     4441 tggtcaggaa attgtgtgaa tgcggagttt gctgattgca gggattaccg ctgtcaatca
     4501 ccagcacttt gcgtaatgcc ctgcccaatg ccatggcagc ggccagtcct gcatagctgc
     4561 cgcccactat tattatatcg tatttttttt cctgtttcat gttgtcaagt agtgatttaa
     4621 agggcagcgg gaaactcatt ccgcctaaca aggtaactgt ttgttttata aaatgtcgnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnatg ttctctggta taaaggcaga cccgataact gctgaccgaa
     4861 gagaaaaaga aagagagtaa tggaaggata gatcaccgcc cagattttca gtgacattat
     4921 taatctcatt tttgttttca tgctgtttag ttttttgatg aattaatttg tcactacaaa
     4981 agtagatggg ataataggag cggtgacagg acgattatgc agttgaacag gacttattta
     5041 aaattcttac ggaaagcctc cggtgaatgc cctgtttgtt ttttaaagaa acggataaag
     5101 taggagacat cttcatatcc cagctggtcg gctatatctt ttacctggcc ggaagtggcg
     5161 agtaaatatc tttttgcttc tagtatgatc tgttcattaa tgagtgcgga cactgttttg
     5221 cccacggctg ctttggcaat ggcattcagc tggtatggcg aaaggtttag caggccggca
     5281 tattgtacca catttttcat gtggctgata ttggtttcca gcaaccgaag cagttcttca
     5341 aaacgatcct gggtataagt gctttgcgta gtggttattt cnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aaaaggctag gtcgaactcc agcaggaaac
     5641 ctgtagcgcc ggcttttaac tccagccggt gtacctgccc gggtcgcagc atgaaaactg
     5701 tattgtcctt tacctggtag ttaacaaaat ctatttcgtg gaggcctttg cctttttgta
     5761 tggccagtac aaagtagaag ttgtgtttat gcaggtcatg taccaggttc tttccgttca
     5821 tcagttcttc cagcttgcgg aagctgaacc ggccggttat atcctgttcg tttggggtgg
     5881 aagtaatatg tctgacaggg atgggtttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac tactacctct tcccgggact
     6001 ttccctgaaa ccaaaaactg catgaactat aattatgacc tccacttcga ttaagtgtag
     6061 tgaaaatttt tataagaaat aaaaatttta atgtatgtga taaaagtgat acgtcaaaag
     6121 tcttcaatta aataattttc ttttccttct tttaacaagt ctctttctat ttcttctagc
     6181 tctttcagtg cttcgtctcg atttatcatg gtactaaaaa ttctagaatt ccttatcaga
     6241 tcagttgttt ttttaaagtt ggttaaagaa tttatactag ttcttggatt atctgtaatc
     6301 t
//
LOCUS       DAORLS010000175         6298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6715_length_6298_cov_1.724011, whole genome shotgun sequence.
ACCESSION   DAORLS010000175 DAORLS010000000
VERSION     DAORLS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_6298_cov_1.724011"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>302
                     /locus_tag="PK977_12745"
     CDS             <1..>302
                     /locus_tag="PK977_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19033.1"
                     /translation="TGIIVQLAISWLIIWMYEKGNLSFLGFYPAKSRLLDFILFFSIT
                     AICCSSGFLLRMYFGNEEWKLNESLTLLTALKATWWNIKSVLFEELIFRGVLFYIL"
     assembly_gap    303..390
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <391..721
                     /locus_tag="PK977_12750"
     CDS             <391..721
                     /locus_tag="PK977_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19034.1"
                     /translation="QMIITFLLTGIMGALYAYGYTKSFSLFIPCGIHLGWNLTQGFIF
                     SQGPIGNGLLIPAKEQPVVTVSYFVYFTILLTPILSTWIINFLVLKKRRQITFTTKPP
                     AQILPAE"
     gene            755..1192
                     /locus_tag="PK977_12755"
     CDS             755..1192
                     /locus_tag="PK977_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF19035.1"
                     /translation="MPQINPHINFNGNAEEAFTFYKSVFGGEFAKLVRFKDIASDEFP
                     VAEKEENKIMHIALPVGKNLLMANDVPESMGKTNENENRSKIAIRAESKEEAVRLFNG
                     LSSGGQIEMPFTESFGDSYFGMFRDKYGIEWMVEFDPNNKGQI"
     gene            1242..1703
                     /locus_tag="PK977_12760"
     CDS             1242..1703
                     /locus_tag="PK977_12760"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRF19036.1"
                     /translation="MLTEEIKKYIDKSVLCWLATCNKDGEPNVSPKEMFTWQDEQTLL
                     IAHIASPNSVANIRSNPNVCVSFVDVFVQKGFKLKGRARIIEKEHAAFPEKVRPLTDL
                     FSDTFPIKDVIEVTVTKAEPIVAPSYFLFPETTEERQIAAAHKTYKVRAAE"
     assembly_gap    1727..1736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1742..>1979)
                     /locus_tag="PK977_12765"
     CDS             complement(1742..>1979)
                     /locus_tag="PK977_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19037.1"
                     /translation="GTAATMNVGTVYTFEGDKLTFGKDGFNNPGKTEITESTFSFQAE
                     GNSYKFIYDYSFNGDTLVASMQNSNGQMLYMVKK"
     assembly_gap    1980..2075
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2243..3280)
                     /locus_tag="PK977_12770"
     CDS             complement(2243..3280)
                     /locus_tag="PK977_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HRF19038.1"
                     /translation="MRNYLLLLTATAFVISCNQPGKKNATGAAMPVSEYFNYGDSGVQ
                     SAGVRMIPISTPVGNFKVWTKRFGNNPRIKILLLHGGPAMTHEYMECFESFFPKEGFE
                     FYEYDQLGSYYSDQPKDSSLWTTERFVEEVEQVRKAIGADSTNFYVLGNSWGGILGME
                     YALKYQQHMKALIVANMMASAPEYGKYSADVLAKQIDPAVLAEIRAIEAKGDVANPRY
                     MELLIPNFYKKHLCRLEEWPDGFNRAMKHVNGEIYTMMQGPSEFGIAGRLAKWDIKNR
                     LKEIKVPALMIGAKHDTMDPKAMEEQSRLVQKGRYLYCPDGSHLAMWDDQQVFMNGVI
                     QFIKDVDQGKF"
     assembly_gap    3505..3627
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3628..4997
                     /locus_tag="PK977_12775"
     CDS             <3628..4997
                     /locus_tag="PK977_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRF19039.1"
                     /translation="DIPNQTQLPANRDELAFYSIPQLAALLINKRISSVELTKFFIER
                     LKKYGDTLECVISLTEDIAMQQAMQADEEIARGVYRGPLHGIPYGLKDLFAVKGTQTT
                     WGSTPYKDQVINEDAFVYKQLRGAGAVLCAKLSLGALAYDNKWFGGATKNPWNLKQGS
                     SGSSAGSAAATSAGLLPFAIGTETWGSIVSPSTRCGVTGLRPTFGTVSRNGGMVLSWS
                     LDKIGPICRSAEDAAIVYAFIHGTDNNDPGSIRHTFNYTGKIEDFKKLRVAYAGNYFK
                     RLAADAPEWKVLDVYRSLGAEVKNVVFPDSTIYPFDIMNVVISAESAAAFDELTRNNR
                     DELIERQDKNFWPNIFRTARTIPAVEYINANRWRSKLCKELRGFMSNFDVVIVPTFAG
                     NQSAMTNLTGHPVVCMPIGFNKNGAPVSITLIGNLYDEATILAAAKAFQDKTDHHQQH
                     PPLFK"
     gene            5014..>5269
                     /locus_tag="PK977_12780"
     CDS             5014..>5269
                     /locus_tag="PK977_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19040.1"
                     /translation="MKYLIPATLLFVLLSCNQKEKADLLVYNATIYTVDSSFSTAEAM
                     AIKDGKIVATGKTADLEKKYDYKEKLDAESKFIYPGFIDAH"
     assembly_gap    5270..5354
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5355..>6298
                     /locus_tag="PK977_12785"
     CDS             <5355..>6298
                     /locus_tag="PK977_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19041.1"
                     /translation="ERVKAFAAENKEGWITGRGWDQNDWDLKEFPNNEMLDTLFPDRP
                     VLLTRIDGHAAIANAKALELAGINPGDTLTGGSIEEMEGSLTGILIDNAIDLVSAKIP
                     NATKEQFKEALKDAEQNCFAMGLTTIDDCGLSYSNVEWIKELHSNGDLKMRLYVMLSD
                     AKENYDYAAKNGMIKTDRLNVRSFKVYGDGALGSRGACLLEPYSDKAGHYGFLLSKPE
                     HFDSVANWLSEKQWQMCTHAIGDSGNRTILNIYAKYLKEKNDLRWRIEHAQVVNQNDF
                     ALFGKYSIIPSVQPTHATSDMYWAGDRLGAERVKGAYA"
BASE COUNT         1692 a         1292 c         1338 g         1574 t
ORIGIN      
        1 acaggcatta ttgttcaact ggccatttcc tggttaatca tttggatgta tgaaaaagga
       61 aacctgtcat tcctgggttt ttatccagcc aaaagcaggc ttttagattt tatactcttc
      121 ttttccatta ctgccatttg ctgctccagc ggcttccttt tgcgaatgta ctttggaaat
      181 gaggaatgga aattaaatga atccttaaca ctgctaaccg ccttaaaagc cacctggtgg
      241 aatataaagt ctgtattgtt cgaagaattg atttttcgcg gagtgctttt ttatattctc
      301 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcaaatgata attacatttt tgctaaccgg
      421 gattatgggt gcactttatg catatggtta cacaaaatca ttctcattat ttattccctg
      481 cggaatacat ttaggctgga acttaacaca ggggttcata ttttcccagg gacctatagg
      541 aaacgggctc ttaataccgg ctaaggaaca gcctgtcgtt actgtgtcat attttgttta
      601 ctttactatc ttattgacac caatattgag tacctggatt attaactttt tagttctgaa
      661 aaaacgaaga caaataactt ttactactaa accgccagcg caaattttac cagctgagtg
      721 aacaagcaat aacaccaatt taaaaattaa accaatgcca caaatcaatc cccacattaa
      781 cttcaacggc aatgccgaag aagcatttac tttttacaaa tcagtatttg gcggggagtt
      841 tgccaaacta gttcgcttta aagacattgc aagcgatgaa tttcctgtag cagaaaaaga
      901 agaaaataaa atcatgcata ttgctttgcc tgtcggcaaa aacttattaa tggccaatga
      961 tgttcctgaa agcatgggca aaacaaatga aaacgagaac aggtctaaaa tagccataag
     1021 ggcagaaagc aaagaagaag ccgtcaggct gtttaacggg ctttcatcag ggggacaaat
     1081 tgaaatgccg tttaccgaga gctttggaga ctcctacttt ggtatgttca gagacaaata
     1141 tggaattgaa tggatggtag aatttgaccc aaacaataaa ggacaaattt aactgatgga
     1201 aaacaacgat tgccttacat gaactgtgca aaaaaccaat catgcttacc gaagaaataa
     1261 agaaatatat tgacaagagt gtgctgtgct ggctggctac ctgcaataag gatggtgagc
     1321 cgaatgtttc gcccaaggaa atgtttacct ggcaggatga gcaaaccctg ctgatcgccc
     1381 atattgcgtc gcccaacagt gtggccaata tccgaagcaa cccgaatgta tgtgtgagtt
     1441 ttgtagatgt gtttgtacag aaaggattta agctgaaagg cagggccagg atcatagaaa
     1501 aagaacatgc tgcattccct gagaaagtaa gacccctgac cgaccttttt tctgacacat
     1561 tcccgatcaa agatgtgata gaagtgacgg tgaccaaggc agagcctata gttgcaccca
     1621 gctatttcct gtttccggaa acaacagaag aaagacaaat agcagcagcc cataaaacct
     1681 ataaggtgag ggctgctgag tagttattaa tctgatctat ttcttcnnnn nnnnnnagta
     1741 gttatttctt caccatatac agcatctgcc cgttgctgtt ttgcatagac gctacaaggg
     1801 tgtcgccgtt gaatgaataa tcgtagatga acttgtagct gtttccttcg gcctggaaac
     1861 taaaggtgct ctcggttatt tctgttttgc cgggattatt gaaaccgtct tttccaaacg
     1921 taagcttgtc gccttcgaaa gtgtacacgg tgcctacatt cattgtggct gccgtgccgn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttac atccccggta ctacccgctt
     2101 ctgtagtagt ttcatttttt gtttccgtac cggtggtttc ttttttgtct ttgctgccgc
     2161 agctgacgaa tacaaaagtt gcgcagccaa tgataaggat gtttttaaag tgcatgggta
     2221 atggttttat gaataaatat aatcagaatt ttccctggtc cacatctttg ataaactgaa
     2281 tgacgccgtt cataaatacc tgctgatcgt cccacatggc gaggtggctg ccatcggggc
     2341 aatagaggta acggcctttc tgcaccagcc tgctttgttc ttccatggct ttggggtcca
     2401 tggtatcatg cttcgcccct atcatcaacg ccggaacttt tatttctttc aaccggtttt
     2461 taatgtccca tttagccagc cggccagcga ttccaaattc agaagggccc tgcatcatag
     2521 tgtatatttc tccgttaaca tgcttcatgg cacggttaaa tccatccggc cattcttcca
     2581 gccggcacaa atgtttttta tagaagttag ggatcagcag ctccatgtag cggggattgg
     2641 caacatcgcc tttggcctca atggccctga tttctgccag cacagcagga tctatttgtt
     2701 tggccagtac atctgctgaa tactttccat actccggtgc gctggccatc atgttggcaa
     2761 cgatcagcgc cttcatgtgc tgctggtact tcagggcata ttccattccg agaataccgc
     2821 cccatgaatt gcccagcaca taaaaattgg tggaatcagc gccgatggcc ttacgtacct
     2881 gctccacttc ttccacaaac cgttcggtgg tccagagact gctgtctttg ggctgatcgc
     2941 tgtaataaga gccgagctga tcgtattcgt aaaactcaaa gccctctttg gggaaaaagc
     3001 tttcgaaaca ttccatgtat tcatgcgtca tggcagggcc accatggagt aataaaattt
     3061 tgatgcgggg attattgccg aatcgtttgg tccatacttt gaaattaccc accggtgtac
     3121 tgatgggaat catgcggaca ccggctgatt gtacgccgct gtcaccataa ttgaaatact
     3181 ccgataccgg catagcggca ccggttgcgt tttttttacc cggctggtta caggagataa
     3241 caaaagcagt ggccgtcagc aggaggaggt aatttctcat agcagctatt ttacaggaag
     3301 ataataattt acttaactta ctaccctcaa agaccggcaa tatgcgatac ttttttctcc
     3361 ttgctttcag cattagctgc atggcagcct catcacaaac caaatcagat tccatcaaat
     3421 tagtacagga tgcattaaag ctctacgatc tttcattcac cgaagccgaa gtggactcga
     3481 tgctggacgg cctgaaagaa aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnggg atataccgaa ccagacccaa ctgcctgcca
     3661 accgggatga actggctttt tattccattc cgcaattagc tgccctgctt atcaataaac
     3721 gcatcagctc agtggagctg acaaaatttt ttattgaacg attaaaaaaa tacggcgaca
     3781 ccttagaatg tgtaatcagc ctgacagaag atattgcgat gcaacaagcc atgcaggccg
     3841 atgaagaaat tgccaggggt gtgtaccgcg gacctttgca tggcatacct tacggactga
     3901 aagacctgtt tgctgtaaaa ggtacacaga ctacctgggg cagcactccc tacaaagacc
     3961 aggtgattaa tgaagatgct tttgtttata aacaactgag aggagccggc gctgtactct
     4021 gcgccaaact ttcactcggt gcattagcct acgataacaa atggtttggc ggcgccacca
     4081 aaaacccctg gaacctgaaa cagggatcaa gcggttcatc ggccggatcg gcagcagcta
     4141 cctctgcagg tttattgcct tttgccattg gtacggaaac atggggttcg attgtttcac
     4201 cttctacccg ctgcggtgta acgggcttac ggcccacctt cggcactgtg agcaggaacg
     4261 gtggtatggt gctgagctgg agtttggata aaatcggtcc tatttgccgc agtgcggaag
     4321 atgcagcgat agtatatgca tttattcacg gaacggataa taatgatcct ggaagtatac
     4381 gccatacctt caactatacg ggcaagatag aggactttaa aaaactgcgg gtagcttatg
     4441 ccggtaatta tttcaaacgg ctggcagctg atgcgccgga gtggaaagtg ctggacgtat
     4501 accgttcctt aggcgctgaa gtgaagaatg tggtttttcc cgattccact atttatccct
     4561 ttgacatcat gaatgtggtg atcagcgctg aatcggctgc cgcatttgat gaactgacaa
     4621 gaaataaccg ggatgaactg attgaaagac aggataaaaa tttctggccg aatatattcc
     4681 gcactgccag gaccatacct gcggtggaat atatcaatgc caaccgctgg aggtcaaagc
     4741 tgtgcaagga actaagaggg tttatgagca attttgatgt ggtgatcgtt cctacatttg
     4801 ccggcaatca aagcgccatg accaacctta ccggccatcc tgttgtttgt atgccgattg
     4861 gattcaataa aaacggagca cctgtcagca ttacgctgat tggtaacctg tatgatgaag
     4921 cgacgatcct ggctgctgcg aaagccttcc aggacaagac tgaccatcat cagcaacatc
     4981 cgccactttt caagtaagat ataatattca agaatgaagt atttaattcc tgccacatta
     5041 ttgttcgtac tcctctcctg caatcagaaa gaaaaagcag acctgttggt atacaatgcc
     5101 actatctata ccgtggactc ctctttctct actgccgaag ccatggcaat aaaagacgga
     5161 aagatagtag caaccggtaa aactgctgac ctggaaaaga aatatgacta taaagaaaaa
     5221 ttagatgccg aaagcaaatt catctatcct ggttttattg atgcccatgn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnngaaaga gtaaaggcgt tcgctgccga aaacaaagaa ggctggatta
     5401 caggccgcgg ctgggaccag aacgactggg atttaaaaga atttcccaac aatgaaatgc
     5461 tggatacttt atttcccgac aggccggttt tactgacgag gattgatggt catgccgcta
     5521 ttgccaatgc caaagctctg gaactggcgg gcattaaccc cggtgatacg ctgaccggcg
     5581 gatcaataga agaaatggaa ggatcactga caggtatact gatcgataat gccatcgacc
     5641 ttgtaagtgc aaaaattccc aacgccacaa aagagcaatt caaagaagca ctgaaagatg
     5701 cagagcaaaa ctgttttgcc atgggcctta ctactattga tgactgcggc cttagttaca
     5761 gtaacgtgga gtggataaag gagcttcaca gtaatggcga tttgaaaatg cggttatatg
     5821 tgatgctgag cgatgcaaaa gagaattatg attatgctgc taaaaacgga atgataaaaa
     5881 cagacaggct aaatgtaaga agctttaaag tgtatggtga cggagcactt ggttcaaggg
     5941 gtgcctgcct gctggaacct tacagcgata aagccgggca ttatggtttc ttattaagca
     6001 agccggaaca ttttgattcg gtggccaact ggctgagtga aaagcagtgg caaatgtgca
     6061 cccatgccat tggcgacagc ggcaaccgca ccattctgaa tatttatgcc aaatacctga
     6121 aagaaaagaa tgatctgcgc tggcgcattg aacatgcaca ggtggtgaat caaaatgatt
     6181 ttgccttgtt tgggaaatac tccatcattc cttcggtaca acctacccat gccaccagcg
     6241 atatgtactg ggccggcgac cggctgggcg ctgaaagagt aaaaggtgcc tatgcata
//
LOCUS       DAORLS010000176         6290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6725_length_6290_cov_2.017322, whole genome shotgun sequence.
ACCESSION   DAORLS010000176 DAORLS010000000
VERSION     DAORLS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6725_length_6290_cov_2.017322"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>427
                     /locus_tag="PK977_12790"
     CDS             <1..>427
                     /locus_tag="PK977_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRF19042.1"
                     /translation="SGTNWMQYPTVAPSSIDHYGFTNIPGWHQSLLVPTLRRGTLYRY
                     KLNSTRNGIVNDSVPYFFRTDRYRDLTLKNGNIIFTVTDSIGSTSGPSGGGTNVLSRP
                     GTILKYTFLGYQNNGANNKSTLPTTIDVTDGPVNTPNSGS"
     assembly_gap    428..487
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <488..1438
                     /locus_tag="PK977_12795"
     CDS             <488..1438
                     /locus_tag="PK977_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF19043.1"
                     /translation="LAEIFANGQTLGTVTSCFYKHSGAIRTQGSVRYLDRNITITPQF
                     QPASPVRIRLYFSKAEFDALDADPLSGITTISDIKILKNQDACGSSVSSTTSLINPVY
                     AEAHGTDGYVVQANVSSFSSFYFGSSNLTLPLHLITFTANFFNGSVLLKWETEQERNT
                     DYFVIERNTGAGFVNIGTIAAGGNTSTRSFYDYTDNKAAGLGFQSAQYRLKTIDKDGS
                     FVYSSVVFISFADLLTRLQINPNPVADGIAKVSITSPAEQQANWKIIDNSGRIVLKGI
                     VQLSRGLNGFSIMTGSLSTGIYYLEVSTRVHNNKIKLQKL"
     gene            1511..>1917
                     /locus_tag="PK977_12800"
     CDS             1511..>1917
                     /locus_tag="PK977_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRF19044.1"
                     /translation="MYTLKKSLGQHFLKDENICRKIVSSLQEHPFKQLLEVGPGGGAL
                     TKYLLKLEDVDFKAVELDEEKVTYLLQTFPELEGKIINESFLDINQPFTGRFTVVGNF
                     PYNISTQILFKILEWRENVEAVVGMFQKEVAQRV"
     assembly_gap    1918..1929
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1930..2282
                     /locus_tag="PK977_12805"
     CDS             <1930..2282
                     /locus_tag="PK977_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRF19045.1"
                     /translation="RVAAKEGSKIYGVLSVLVQAFYKVEYLFDVHENCFQPPPKVKSG
                     VIRLLPVKAVPQMKSEQYFFQLVKTAFNQRRKTLRNAVKSLFDAADLQDELFNKRAEQ
                     LTVQQFAELTFKMK"
     assembly_gap    2301..2312
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2337..>2677
                     /locus_tag="PK977_12810"
     CDS             2337..>2677
                     /locus_tag="PK977_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRF19046.1"
                     /translation="MKDRLEEKGHEVLYLPSITYDELAEIIPSATGIVVTTRITVDRN
                     LIDKGTSLEWVGRLGSGMELIDVVYAESKGIRCVSSPEGNRNAVAEHTLGLLLNLLNN
                     INASSEDIKKG"
     assembly_gap    2678..2687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2688..3243
                     /locus_tag="PK977_12815"
     CDS             <2688..3243
                     /locus_tag="PK977_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF19047.1"
                     /translation="WLRDENRGTELSGKTIGIIGYGNTGSSLAKLLSSFDVTVLAYDK
                     YKTGFAKDYIKEASFEQICRYADVISFHLPLTTETHHLANGSFFSSLQRKPWILNTSR
                     GKVVAIPDLIEALKDNRISGAGLDVLENEKLATYSSSEKEQLDWLLSQHNVIITPHIA
                     GYTHEASYKMAAVLLTKLGLIGYQ"
     gene            3313..3762
                     /locus_tag="PK977_12820"
     CDS             3313..3762
                     /locus_tag="PK977_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19048.1"
                     /translation="MRRNLLLIIFVLVLVITSCIKPLEDRITGNWKLTDSFRRGFFDR
                     DYFQTGYESGIFSFHEGGSATYISNTDTLTGSWKVRRYSDNYFNSSTGQWESKQLKSL
                     RITLVKFSQNRFIDWFFDDFNFRNNSHRIRAEEFIPGGDRVYEFEKR"
     gene            3886..4359
                     /gene="greA"
                     /locus_tag="PK977_12825"
     CDS             3886..4359
                     /gene="greA"
                     /locus_tag="PK977_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRF19049.1"
                     /translation="MADIQYVTKETLEQMKAELQRMKTVDRPAASKAIAEAREKGDLK
                     ENAEYDAAKEAQGLLEAKIAAMEGSMANIRVIDESSIDTSKVSILTKVTLTNLGTKKQ
                     VTYKIVSEKEADLKAGKISVTSPIGKGILGKTVGETAEVQAPAGILKFKIEEITA"
     gene            4499..4888
                     /locus_tag="PK977_12830"
     CDS             4499..4888
                     /locus_tag="PK977_12830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRF19050.1"
                     /translation="MTIFSKIIAGEIPSYKIAENEKFFAFLDIFPLREGHVLVVPKTE
                     TDKLFDLSDDYLSEMLVFAKPIAHAIEKAFHCNRCGISVIGLEVPHAHMHLIPINSSN
                     DLNFTQHKPQASAERLKKAQEKILAVL"
     gene            complement(4892..5455)
                     /gene="ruvC"
                     /locus_tag="PK977_12835"
     CDS             complement(4892..5455)
                     /gene="ruvC"
                     /locus_tag="PK977_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRF19051.1"
                     /translation="MQKPSKIILGIDPGTLVMGYGLITVKDNKVSLLELGVLKPGKVK
                     DNYKKLQLIFNTVSGLITKYQPTDFAIEAPFFGKNVQSMLKLGRAQGVAIAAAMRHGL
                     EVTEYSPKKVKQSITGNGNADKEQVMKMLQTLLSFKENPRHYDATDALAVALCHHFQN
                     SSPINKAVKGVKGWEDFIKKNPGRIKK"
     gene            5497..5697
                     /locus_tag="PK977_12840"
     CDS             5497..5697
                     /locus_tag="PK977_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19052.1"
                     /translation="MNETKQKYKTLHQLLPWAFVVHLVVLVNLQANTTAAGIGAGLAT
                     NESINERTDALELIAGVYIDDR"
     assembly_gap    5899..5953
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6010..>6290
                     /locus_tag="PK977_12845"
     CDS             6010..>6290
                     /locus_tag="PK977_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19053.1"
                     /translation="MKVILYGVWGVFLVLTLFYFRLSWIIKWVDRLPGSKRFAYLVRA
                     LEDFNATILLKLMSLSLLRFFVFIIQYYLLFVLFDVNVSGWQAFWTVSVS"
BASE COUNT         1863 a         1078 c         1342 g         1858 t
ORIGIN      
        1 atcggggact aactggatgc aatatcccac agtagcacca tcaagtattg atcattatgg
       61 atttacaaat attcctggct ggcaccaatc actgcttgta ccgactttgc gaagaggtac
      121 tttgtacagg tataaactaa acagcacaag aaatggtatt gttaacgatt ctgttcctta
      181 tttcttcagg actgatcgtt accgtgatct cacattgaag aatggaaata tcatatttac
      241 tgttacagat agtattggtt ccacttctgg tccctcaggc ggaggaacca atgttctttc
      301 gaggcccgga accattttga aatatacttt tcttggctac cagaataatg gcgcaaacaa
      361 taagagtaca ttaccaacaa ccatagatgt tacagatgga ccggtgaaca ctccaaacag
      421 cggctcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnctg gctgaaatat ttgccaacgg acaaacgctg ggtaccgtta cctcttgttt
      541 ttacaagcac tcaggtgcca tcagaacgca gggctccgtt aggtaccttg accgtaacat
      601 aacgatcacc cctcaatttc agcccgcatc cccggtacgg atcaggttgt atttttccaa
      661 agctgagttt gacgcattag atgctgatcc attgagtggc attactacta tctctgatat
      721 taaaatatta aaaaaccagg atgcatgcgg gagcagtgta tcttcaacaa catcactgat
      781 caaccctgta tatgccgagg cacatggaac ggatggttat gttgtgcagg caaatgtttc
      841 ttccttttca tctttttatt ttggttcatc aaaccttact cttcccttgc atctcataac
      901 atttacagca aactttttta atggttctgt attacttaaa tgggaaacag agcaagaaag
      961 aaatacagat tattttgtaa ttgagagaaa tacgggagcc gggtttgtaa acattggaac
     1021 tattgctgca ggtggtaata cttcaaccag atctttctat gattatactg ataataaagc
     1081 agcaggcctg ggatttcagt ctgcacaata taggttgaag actattgata aagacggaag
     1141 ttttgtttat tcatcagttg tgtttatttc atttgcagac cttttgacca ggttacaaat
     1201 taaccccaat cctgtggcag atgggatagc caaagtttct ataacttctc ctgcagaaca
     1261 gcaggcaaat tggaagatta tcgataattc tggcagaatt gtacttaagg gtattgtgca
     1321 attatcaagg gggttgaatg gtttttcaat tatgacaggt agtttatcaa ccggcattta
     1381 ttatttggaa gttagcaccc gggtgcataa taataagatc aaacttcaaa aactataatg
     1441 tatacataaa tcacaggcag agtgtctgac ataaggcccg aagtaggaat aatcggctac
     1501 ttttgtatca atgtatacat taaaaaaatc actgggacag cactttctga aggatgagaa
     1561 catatgccgg aagattgttt cttcactaca ggagcatcct tttaagcagc tattagaagt
     1621 tggtcccggc ggaggtgctc taacaaaata cctgctgaaa ttagaggatg ttgattttaa
     1681 agctgtggag ctggatgaag aaaaagtgac ttacctgctt cagacatttc cagagttgga
     1741 agggaaaata atcaatgaaa gctttctgga tataaatcaa ccttttaccg gaagatttac
     1801 tgtcgttggt aattttccct ataatatttc aacccagatt ttgtttaaaa tactggaatg
     1861 gagagaaaat gttgaagcag tggtggggat gtttcaaaaa gaagtagcac aaagggtnnn
     1921 nnnnnnnnna aagggtggca gcaaaagaag gcagtaaaat atacggagta ctcagtgtat
     1981 tagtgcaggc tttttataaa gttgaatact tgtttgatgt gcatgagaat tgttttcaac
     2041 cgccgccgaa ggtgaaaagc ggggtcataa gacttcttcc ggttaaagca gttccccaga
     2101 tgaaaagtga gcaatatttt tttcaattag tcaagacagc tttcaatcag cgaagaaaaa
     2161 cattgagaaa tgcagtgaaa agcctttttg atgcagcaga tttgcaggat gagttgttta
     2221 ataaaagagc agagcaattg acagtgcaac aatttgcgga gcttactttc aaaatgaaat
     2281 gaaaaaggta attattacgg nnnnnnnnnn nnattacggg aaaatcacat ccgatattga
     2341 aggaccgcct ggaggaaaaa ggccatgagg tgctttatct tccttcaatt acatacgatg
     2401 agctggcgga aataattcct tcagcaacgg gtatagtggt aaccactcgt atcacagttg
     2461 atagaaatct tatagataag ggcacttctt tagaatgggt tggcagactg ggcagtggta
     2521 tggaactgat agatgttgtt tatgctgaaa gcaaaggaat ccgctgtgta agcagccctg
     2581 aaggtaaccg taatgcagta gcagaacata cattggggct tttgttaaac ctgcttaata
     2641 atattaacgc ttcatcagag gatataaaaa aaggtttnnn nnnnnnnatg gctgcgggat
     2701 gaaaacaggg gaacggagct aagcggcaaa acaattggaa ttattggcta tgggaataca
     2761 ggaagtagct tagcaaagct gctttcgtct tttgatgtaa cagttcttgc ttacgataag
     2821 tataaaacag gttttgccaa agactatatt aaggaggcaa gctttgaaca aatttgccgg
     2881 tatgccgatg ttatcagctt tcacctgcct ctgacgacag aaacacatca tctggcgaat
     2941 ggtagttttt ttagttccct gcaaagaaag ccatggatat tgaacacatc acggggaaaa
     3001 gtagtggcca ttccggattt aattgaagcg ttaaaagaca atcgtatctc aggagctggc
     3061 ctggatgtgc ttgagaatga aaaattggcc acctattctt cttcagaaaa agaacaattg
     3121 gattggttac tgtctcagca caatgtgatc ataacaccac acatcgccgg ttatacgcat
     3181 gaggcttctt ataaaatggc tgctgttctg ttaaccaaac tgggcttaat tggttaccag
     3241 taatcttttg ttaatatttc ccaggataat tacagtggta tatttttttc ggcaattgag
     3301 gaaacaattg ttatgagaag aaatttactc ttaatcattt ttgttttagt ccttgtcatc
     3361 acttcctgta taaagccatt ggaagatcgt ataacgggta attggaagtt aaccgattct
     3421 tttcgtcgtg gattttttga ccgggactat tttcaaaccg ggtacgaatc cggaattttt
     3481 tcttttcatg aaggcggttc tgccacctac atcagtaata cggatacatt gacaggttca
     3541 tggaaggtga ggcggtatag cgataattat tttaacagtt ccaccgggca gtgggaatca
     3601 aagcaattga aatctttaag gattacttta gtcaagttct cacagaacag gtttattgac
     3661 tggttttttg atgatttcaa cttcagaaat aacagccata gaatcagggc agaagagttt
     3721 atacccggtg gcgacagggt atacgaattt gaaaaaaggt aactgtaaac tgataaagat
     3781 gttttgcttt ttcaaatagt tgtgtatctt tacaactcaa tattgcaacg cttcagccca
     3841 agaactgagg cgttgttatt tttttattta tcaaaggagg tgactatggc agatattcag
     3901 tatgtgacga aagagacgct ggaacaaatg aaagcagaat tgcagcggat gaaaactgtt
     3961 gacaggcctg cagccagtaa agctattgca gaagccaggg aaaaaggcga tctaaaagag
     4021 aatgcggagt atgatgccgc taaagaggcg caagggcttt tggaagctaa aattgcagca
     4081 atggaaggat caatggccaa catcagggtg attgatgaat caagtattga taccagtaaa
     4141 gtttcaatac ttacaaaagt aacgttgact aacctgggaa ctaaaaagca ggtaacttat
     4201 aagatcgtaa gtgaaaaaga agctgatctg aaagcaggca aaatttcagt tacgtctcct
     4261 ataggaaaag gtattttagg taaaacagtt ggagaaactg ctgaggtgca ggccccggca
     4321 ggaatactga aattcaagat agaagaaatt actgcttaaa aacccttgag cattagcagt
     4381 agcccggctt ttttgccggg ttttttatgc attatagttt tagctttgga atcaatatca
     4441 gatgatcagg cgtatcagat attatctttt atcattaaaa aataaaatct ttggtggtat
     4501 gactattttt tcaaaaatca ttgccggtga aattccttct tataaaattg cggagaatga
     4561 aaaattcttt gccttcctgg atatttttcc gctgagggaa ggacatgtgc tggtagtgcc
     4621 taagacagaa accgataaac tgtttgatct ttcagatgat tacttatcag aaatgcttgt
     4681 ttttgccaaa ccaattgcgc atgccattga aaaggctttt cattgtaatc gttgtggcat
     4741 aagcgtcatt ggactggaag tgccacatgc acatatgcac cttattccca ttaattcttc
     4801 caatgatctc aattttacac agcataaacc acaagccagt gcagagcgtt tgaaaaaggc
     4861 acaagagaaa atactggcag tgctataatt atcatttttt aatcctgcca ggattttttt
     4921 tgataaaatc ttcccagcct tttacacctt taaccgcttt gtttattggg gaactgttct
     4981 gaaagtgatg gcataatgct accgctaatg catcggttgc atcataatgc ctgggatttt
     5041 ctttaaaaga caacagcgtt tgaagcatct tcataacctg ttccttgtct gcattgccat
     5101 ttcctgtaat agattgtttc acttttttag gtgagtattc tgttacttcc agtccatggc
     5161 gcattgctgc agcaatagca actccctgtg ctcttcccaa tttcagcatg ctttgaacgt
     5221 ttttaccgaa aaagggtgct tcaatggcaa agtcggttgg ctggtatttg gtgatcaggc
     5281 cactgacagt attgaaaata agttgcagct ttttataatt gtctttcact ttgcctggtt
     5341 taagtacacc gagttcaagc aggctcactt tattatcctt tactgtaata agaccataac
     5401 ccatgaccag agtgccggga tcaataccaa ggattatttt agaaggcttt tgcaagcagg
     5461 ctgtattttt acaaaatccc actaattgcg ggaggggtga atgaaactaa acaaaaatat
     5521 aaaactcttc atcaattact tccttgggcc tttgttgttc atttggttgt cctggtcaat
     5581 ttacaggcaa atacaacagc agccgggatt ggagcaggcc tggcaacgaa tgaaagcatc
     5641 aatgaacgga ccgatgctct ggaacttatt gctggtgttt atattgatga tcgctaattg
     5701 gtcaattgag gctgtgaaat ggcagttgtc tgtaaaacaa attcaaaaag taagtttcgc
     5761 caggtcattg aaggctgtat tatccggtgt ttctttctcg gtaacaacgc caaaccgtgt
     5821 aggtgaatac ctggggaggg ttttgtacat ggatgaggga aacaggttga agacaatttc
     5881 gctgaccatt gttggaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnngccttat cattttacgc cccatattag aggagcagca aatcatttct
     6001 gtagtatgga tgaaagtaat cttgtatggt gtgtggggtg tttttcttgt tttaacacta
     6061 ttttattttc gattatcatg gataataaaa tgggtagatc gtttaccggg tagtaagcgg
     6121 tttgcttacc ttgtcagggc tttagaggat ttcaatgcaa caatactgct taaattaatg
     6181 tcattatctc tgttaaggtt ttttgtattt attattcagt attatctgct ctttgttttg
     6241 tttgatgtga acgtttcggg gtggcaggct ttttggacag tcagtgtttc
//
LOCUS       DAORLS010000177         6248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6770_length_6248_cov_1.843856, whole genome shotgun sequence.
ACCESSION   DAORLS010000177 DAORLS010000000
VERSION     DAORLS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6770_length_6248_cov_1.843856"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK977_12850"
     CDS             complement(<1..270)
                     /locus_tag="PK977_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRF19054.1"
                     /translation="MNVITNNSIRCTIALEALEQVVDPEIGLNVVDLGLIYQLDFDEE
                     HKKIFVTMTLTTQFCPMGESIVDGVTQALQQTFPDDTIQVDLTFDP"
     gene            complement(267..>1100)
                     /locus_tag="PK977_12855"
     CDS             complement(267..>1100)
                     /locus_tag="PK977_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HRF19055.1"
                     /translation="ILTASLWLFLTAFLGLALVYNFTFPFLPKETLQYLPLHAHVGII
                     GWFLLLIIGVGSRLIPMFLISKYANSKLLWVIYFLINAGLLSFMLSFLYFPKREYYNF
                     SIAAVVGALLLFGYYCYCAVKERIRRQVDEQMKTSLLSVMMMLLPLLFLVVITGWLVA
                     DQISARMVLIYGFAIFFGWITAIIFGMTFKTLPFIVWNKVYHDKAGLGKTPNPKDLFS
                     NKLFILMVLIYLGGFILFIAGAYLADTYLLNFAAAILLLAAVLYNWNVMKVLFHKPKK
                     L"
     assembly_gap    1101..1110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1111..1561)
                     /locus_tag="PK977_12860"
     CDS             complement(<1111..1561)
                     /locus_tag="PK977_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HRF19056.1"
                     /translation="MFGSGFNNAAGRNTTHKIVLPFYAYAAVSLLVSTVLLFFSAEAF
                     TQHYFHPHTLAITHMMALGWGTMIILGASHQLVPVLIEKNLYSVRLGYASFFMAAVGI
                     PLLVYGFYVFDMGWPAKWGGRFVLLSVICYIINLAASMSEGRKVNVHA"
     gene            complement(1563..>1789)
                     /locus_tag="PK977_12865"
     CDS             complement(1563..>1789)
                     /locus_tag="PK977_12865"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRF19057.1"
                     /translation="YNGKMNVVDVRNLEMPKPMMTILESLNKLEAGKALFVQHKRIPV
                     FLLPELADRGLDYRINEVREGDVQLLIFKP"
     assembly_gap    1790..1809
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1810..2137)
                     /locus_tag="PK977_12870"
     CDS             complement(<1810..2137)
                     /locus_tag="PK977_12870"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRF19058.1"
                     /translation="MKAMPSLLQDINPQRLVSLDVRPILAAGADPLTLILGKIKELQT
                     GQVLEIVNTFEPRPLMGLLKKQGFDSYAAIIHDDLVKTYFYRETETPVNNSEGNEDKA
                     DGWNDLM"
     assembly_gap    2188..2205
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2206..2404)
                     /locus_tag="PK977_12875"
     CDS             complement(<2206..2404)
                     /locus_tag="PK977_12875"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRF19059.1"
                     /translation="MIINANTKIATVLKAHPGALEAIISISPKFQKLRNPILRKLMAG
                     RTSLAMASKVGGCTINEFFEKL"
     gene            complement(2404..>2606)
                     /locus_tag="PK977_12880"
     CDS             complement(2404..>2606)
                     /locus_tag="PK977_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19060.1"
                     /translation="ATLQSGLDKMISECRMKGPDHDALHKWLEPLIGQVKKLNETEKP
                     EESAQQLTAITQQIQLYFQFFE"
     assembly_gap    2607..2618
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2619..2860)
                     /locus_tag="PK977_12885"
     CDS             complement(<2619..2860)
                     /locus_tag="PK977_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19061.1"
                     /translation="MKLFFISLGLLLIAACNNQQSAPAADEHKAADQHEHNLPAESLR
                     LNNGVKWKADSSTNNNVKALQAIVEQFTSTEKTPGT"
     gene            complement(2863..3831)
                     /gene="ric"
                     /locus_tag="PK977_12890"
     CDS             complement(2863..3831)
                     /gene="ric"
                     /locus_tag="PK977_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRF19062.1"
                     /translation="MQPVAENILNVTLLEPRLKHPTIFNRFDELAEGDSLTIHNDHDP
                     KPLYYQMLGERGNIFTWEYLEQGPEWWKVRISKKISGEQDETLGQIAAKDLRKAQIFK
                     KYGLDFCCGGKKTVKEACKEKGIDVTKIEQELQQTDKLAATARPLPYNDWNLDFLCDY
                     IVNTHHSYVLKTLPDISAYADKVNRVHGGRHPELGPISLLVQNTYNEMMSHMVKEEKV
                     LFPYIKALVAATNGVQPLQASHFGTVQNPINMMEMEHEVVGNNLAEIRNLTNDYTLPE
                     DACASYSLLYRLLEEFEEDLHLHVHLENNILFPKALKLEKDHLKNN"
     gene            complement(3896..4339)
                     /locus_tag="PK977_12895"
     CDS             complement(3896..4339)
                     /locus_tag="PK977_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRF19063.1"
                     /translation="MNEKIIAFITKQTCGNLCCVTAKGKPWCFSFFYAFNKEEGLLYY
                     KSSEDARHSAIIKANPFVAGTILPDKLNLLQIKGVQFEGTVIPFEHSLAKGAYSAYHK
                     KFPFSIAMPGQIWTIRLDEIKFTDNSLGFGTKLKWERNEMSLNSK"
     gene            complement(4336..4557)
                     /locus_tag="PK977_12900"
     CDS             complement(4336..4557)
                     /locus_tag="PK977_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRF19064.1"
                     /translation="MHKIIHYRNKCIGCGVCYEQQPELWRMSKKDGKATLIKGIGKKD
                     VFVLSISNDAVHRSQEVAVACPVRIIKIT"
     gene            complement(4550..>5615)
                     /locus_tag="PK977_12905"
     CDS             complement(4550..>5615)
                     /locus_tag="PK977_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRF19065.1"
                     /translation="GIRCYLTLNTVMYEHDMQLLQAILKEVKKCKIDAVIASDFAVIE
                     YCSQHGIPLHISTQANVSNIESVKFFSRFANVIVLARELTLKQVQHITDEIKRKKIKG
                     PSGELIKIEIFVHGALCMAVSGKCYMSLHTQNASANRGACVQNCRRPYIVTDAETGEE
                     LKIEGEYIMSPKDLCTIDILDQVIASGADVLKIEGRTKGADYVQVVTKCYREAIDEIA
                     DGTYSKEKIEEWKGELTKVYNRGFWEGYYLGRQLGEWTPQPGSAATEKKLYIGKGSKY
                     YPKIGVGEFIIEAGMLKVGDTLMITGPKCGMVKEKLGELTVNGEKAMEAVKGDKITFP
                     FVTKVTPADKLYKIVEAAHA"
     assembly_gap    5616..5661
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5815..>6248)
                     /locus_tag="PK977_12910"
     CDS             complement(5815..>6248)
                     /locus_tag="PK977_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19066.1"
                     /translation="KNSGADALVLFNRFYQPDFDINELVVKTDLHYSESSEIRLPLMW
                     IALLYGKVKLSLAATTGVQSAVEVVKYLLAGADAVMTASSLYKNGIAYLKTMNKELQD
                     WMYMMGFDSIDKFRGIMSQQNVSDPTAFERANYIRILEGVK"
BASE COUNT         1738 a         1442 c         1127 g         1835 t
ORIGIN      
        1 cgggtcaaag gtaagatcaa cctgtatggt atcatcggga aaagtttgct gcaaggcctg
       61 tgttacaccg tctacgatgg attcacccat aggacaaaac tgggttgtca gggtcattgt
      121 tacaaatatt tttttatgtt cctcatcaaa atccagctgg tagatcaacc cgagatccac
      181 cacattcagt ccgatctccg gatcaactac ctgttccaat gcctccagtg caatggtaca
      241 cctgatagaa ttattagtga tgacattcat aatttttttg gtttatgaaa taatactttc
      301 atcacattcc agttgtataa aactgcagct aagagcaaaa ttgccgccgc aaaattcaaa
      361 agataagtat ctgccaggta agcccctgct ataaatagaa tgaaaccgcc caggtagata
      421 agcaccatca atataaatag cttattactg aaaaggtctt tgggattggg ggttttacct
      481 aaccctgcct tgtcatgata cactttattc cagacaataa atggcagtgt tttaaacgtc
      541 atcccaaaaa taatcgccgt gatccagccg aagaaaatgg caaagccata tatcagcacc
      601 atcctggcac ttatctggtc tgctaccagc cagccggtta taactactaa gaaaagcagc
      661 ggcagcaaca tcatcatcac agaaagcagg gatgttttca tttgttcatc cacctgtctt
      721 cttatcctct ctttcactgc acaataacag tagtacccaa agagcaacaa agcccctact
      781 acggcagcaa tggaaaaatt gtaatactcc cttttcggga aataaagaaa actgagcata
      841 aagctgagta accctgcatt gatgaggaaa taaataaccc agagcaattt gctgttagca
      901 tattttgaaa tcaggaacat gggaataagc cttgaaccca ctccgattat caataaaaga
      961 aaccagccaa tgatacccac atgggcatgc agcggaaggt attgcagtgt ttcttttggt
     1021 aaaaacggaa aagtgaaatt atacaccaat gccagtccca gaaaagcagt gaggaataac
     1081 cacaaagaag ccgtgagaat nnnnnnnnnn ccgcatgcac atttactttt cgtccctccg
     1141 acatacttgc tgccaggtta atgatatagc agatcactga aagcagtaca aaccggccac
     1201 cccattttgc cggccagccc atgtcaaaca cataaaagcc atatactaat aaaggtatgc
     1261 ccacagcagc catgaaaaag gaagcatagc ctaatctgac actgtacaaa tttttctcaa
     1321 tcagcaccgg taccaactgg tgactggcac caagtataat catggtaccc cagcccaacg
     1381 ccatcatatg agtgatagct aacgtatgcg gatgaaaata atgctgtgtg aatgcctctg
     1441 cagaaaaaaa taaaagtacg gtagaaacaa gcagcgaaac agcggcataa gcatagaagg
     1501 gcagtactat tttatgtgtg gtattcctgc ctgctgcatt attaaagccg cttccaaaca
     1561 tttcagggtt taaaaattaa aagttgaaca tcgccttctc tcacttcatt gatgcgataa
     1621 tcaagccccc ggtctgccag ctccggcaat aaaaatacgg gaatacgttt atgctgtaca
     1681 aataatgcct taccagcctc aagcttattg agcgactcca gtatagtcat cattggcttc
     1741 ggcatttcca ggttcctcac atctacaaca ttcatcttgc cgttatatcn nnnnnnnnnn
     1801 nnnnnnnnnc catcaggtca ttccatccat cagccttatc ctcatttcct tcactattat
     1861 tcacaggcgt ctcagtttcc ctgtagaaat aagtcttgac caaatcatca tgaatgatag
     1921 cagcatagga atcaaagccc tgctttttta ataatcccat taatggcctc ggctcaaacg
     1981 tattgacgat ctccaatacc tgcccggtct gcaattcttt tatctttcct aaaataagtg
     2041 tcagcggatc agcacccgct gcaagtatag ggcgcacatc taatgagacg agtcgctgcg
     2101 ggttaatatc ctgcagtagt gatggcattg ctttcatatc ctttatttta gatggttgaa
     2161 cagattcatc aattttaaac cccagtgnnn nnnnnnnnnn nnnnngaagt ttttcaaaaa
     2221 actcatttat ggtacaccct cccacttttg atgccatggc caggctggtt cttcctgcca
     2281 tcagtttgcg aagaatcggg tttctcagct tttgaaactt cggactgatg ctaataatcg
     2341 cctccagtgc acccggatga gctttcagta ctgtagcaat cttagtatta gcatttatga
     2401 tcattactca aaaaattgaa aataaagttg gatttgctga gtgatagcag taagctgttg
     2461 agccgactct tccggttttt cagtttcgtt taacttttta acctggccaa ttaaaggttc
     2521 cagccattta tgaagtgcat catgatcagg ccctttcatc cggcattcac tgatcatttt
     2581 gtcgagcccg gactgcagtg ttgctcnnnn nnnnnnnnta agtccctggt gttttttcag
     2641 tactggtaaa ttgctcaacg atggcctgca aagctttcac attattattt gtactgctgt
     2701 cagccttcca tttcactcca ttattaagac gtaatgattc tgccggcaaa ttgtgttcat
     2761 gctgatcagc cgctttatgc tcatctgcag ccggtgctga ttgctgatta ttacaggcgg
     2821 caattaacaa gagcccaaga gaaataaaaa aaagtttcat ggtcaattat tttttagatg
     2881 atctttctcc agtttcagcg ccttcggaaa gagaatattg ttttccaaat ggacatgcag
     2941 gtgaagatct tcctcaaact cttccaataa cctgtacaac aaactgtaac tggcacaggc
     3001 atcctccggt aatgtgtaat cattagtgag gtttctgatc tctgcaaggt tatttcctac
     3061 aacttcatgc tccatttcca tcatattgat gggattttgt accgtaccaa aatgagatgc
     3121 ctgcaatggc tgcactccat tagtagctgc taccaacgct ttgatatagg gaaacaatac
     3181 tttctcttct tttaccatat ggctcatcat ttcattgtaa gtattttgca ccaggaggct
     3241 gataggtccc agttcgggat gacggccacc atgcacacgg ttcaccttgt ctgcataagc
     3301 actgatatcg ggaagcgtct tcaatacata gctgtgatgt gtatttacaa tataatcgca
     3361 aagaaaatcg aggttccagt cattataggg taagggccgg gcagtagctg ccagtttatc
     3421 cgtttgttgt aattcctgtt caatttttgt tacatcaata cctttttcct tacaagcctc
     3481 tttcaccgtt ttcttaccac cacaacaaaa atcgagaccg tattttttaa aaatttgtgc
     3541 cttccgaaga tccttggcgg caatctgtcc caatgtttca tcctgttcgc cactgatttt
     3601 tttgctgatc cttactttcc accattctgg tccttgttcc aggtattccc aggtaaaaat
     3661 atttcccctt tcacccagca tctggtaata gagtggtttg ggatcatgat cattatgaat
     3721 ggtaaggcta tctccctcag ccagttcatc aaagcggtta aaaatagtag ggtgcttaag
     3781 gcgtggttcc agcaaggtta cattgaggat attctctgct actggttgca tatcggttgt
     3841 tttaatgtga aaacaaagat atgcaaaatt caataataaa agtatttaaa tacttttatt
     3901 tactgtttaa tgacatttca ttccgctccc acttgagttt ggtgccaaaa cccagtgagt
     3961 tatcagtaaa ttttatttcg tccagcctga tagtccagat ttgccccggc attgcaatgg
     4021 agaacggaaa ttttttatga taggctgaat aggcaccttt tgccagggag tgctcaaaag
     4081 gaatgacggt tccctcgaac tgaactcctt taatctgcaa aagattaagt ttatccggca
     4141 gaatggtacc cgcaacaaaa gggtttgcct ttatgatagc tgaatgcctt gcatcttctg
     4201 atgatttgta gtacaacaag ccttcttcct tattaaatgc ataaaaaaaa ctgaagcacc
     4261 agggcttccc ttttgcagta acacaacaca ggttaccaca ggtctgtttg gtgataaatg
     4321 caatgatctt ttcgttcatg tgatcttaat tattcttaca gggcaggcaa ccgctacttc
     4381 ctgcgagcgg tgtacagcat catttgaaat ggacaacaca aatacatctt tctttccaat
     4441 tcctttgatc aaagtagcct tgccatcttt ttttgacatc cgccacaact ctggttgttg
     4501 ttcataacat acaccacacc cgatacattt attccggtaa tgaatgatct tatgcatgag
     4561 cggcctctac tattttatag agtttatcgg caggtgttac ttttgtaaca aaaggaaaag
     4621 taatcttatc tcctttcaca gcctccattg ccttctcacc atttacggta agctccccta
     4681 gtttttcttt gaccattccg catttaggac cggttatcat taatgtatcg ccaaccttca
     4741 gcatacctgc ttcaatgatg aattcaccta cacctatctt gggataatat ttactcccct
     4801 taccgatgta caatttcttc tccgtagcag cagaaccagg ctgcggagtc cattcgccta
     4861 attgacggcc gagataatat ccctcccaaa aacctcggtt ataaactttt gtcagctccc
     4921 ccttccattc ctcaattttt tcctttgaat aagtgccatc agcgatttca tcaattgctt
     4981 cccggtagca tttcgtcacc acctgtacat aatcagcacc ttttgttctt ccttctatct
     5041 tcaatacgtc tgcaccgctt gctattacct ggtccaaaat atcaattgta cacaggtctt
     5101 tgggcgacat gatatactca ccctcaattt ttagttcttc tccggtttct gcatcagtaa
     5161 cgatatatgg cctgcggcaa ttctgcacac aggcacccct gttggccgaa gcattttgtg
     5221 tatgcaggct catgtagcat ttaccggaaa cagccatgca taaagccccg tgaacaaata
     5281 tttctatttt gatcagctca ccggaaggac cttttatttt cttacgcttt atttcgtcag
     5341 ttatgtgctg cacctgcttt aaggttagct cccttgccaa aacaatcaca ttagcgaaac
     5401 gggaaaagaa tttgactgac tcgatattgc ttacattggc ttgcgtagaa atatgcagcg
     5461 gaatgccatg ctgactgcaa tattcaatca cagcaaagtc agatgcaatg actgcatcta
     5521 ttttacactt cttaacctct ttcaggattg cctgcagtaa ttgcatatca tgttcataca
     5581 tcaccgtgtt taaggtgaga tagcagcgaa tacccnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn ngatatataa aatgactgtg ccgacctggt acgcatattc
     5701 agttgctcta ccccaaaata gatggtatca gcccctccgt tgatagcggc ttgtaaactg
     5761 tcaaaagagc cgaccggtgc cagcaattct actttttgtt tcttcatacc gaatctattt
     5821 cacaccttcc agtatcctta tataatttgc cctttcaaaa gcagtaggat cagatacatt
     5881 ttgctggctc ataattcccc tgaacttatc aatggaatca aagcccatca tatacatcca
     5941 gtcctgcagc tctttattca tcgtctttaa gtaagcaata ccgtttttgt acagtgaaga
     6001 agctgtcatt acagcatcag cgccggccag caggtatttc actacttcta cagcgctttg
     6061 cacacctgta gtagcagcca atgataattt tacttttcca tagagcaagg cgatccacat
     6121 caatggcaac cttatttcgc tggattcact gtaatgcaga tcagtcttaa ctaccagttc
     6181 attgatatca aagtccggct ggtaaaaacg gttaaacaag accaatgcat cggccccgga
     6241 attcttca
//
LOCUS       DAORLS010000178         6239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6780_length_6239_cov_2.166397, whole genome shotgun sequence.
ACCESSION   DAORLS010000178 DAORLS010000000
VERSION     DAORLS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_6239_cov_2.166397"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>335)
                     /gene="mreC"
                     /locus_tag="PK977_12915"
     CDS             complement(<1..>335)
                     /gene="mreC"
                     /locus_tag="PK977_12915"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRF19067.1"
                     /translation="GIKDNMAVLGSDGSAVGVVVNVSPNFSQAMSLLHVQHKVNASLK
                     KSGNFGTVEWDGKDPRYLTLRGIPQSVEVNKGDTVLTSTYSFNFPPGHMVGYIDEVIK
                     DKSTNFYVL"
     assembly_gap    336..403
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<404..751)
                     /locus_tag="PK977_12920"
     CDS             complement(<404..751)
                     /locus_tag="PK977_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRF19068.1"
                     /translation="MRNVLLFIRRYRTFFAFLILQGVALWFLFSYNRFHRAKFLGVAN
                     EVTGVINSQYNKVEDYFTLREENKRVNRLNDSLLNLLPSHYGRRDTTVSAVQDSVPYD
                     TLGHYRRYFSRPAT"
     assembly_gap    758..812
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(846..1970)
                     /locus_tag="PK977_12925"
     CDS             complement(846..1970)
                     /locus_tag="PK977_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRF19069.1"
                     /translation="MKIVKNLCQTRVSALLIQIISSTFAPTKSKEILMGLFNWFTQEI
                     AIDLGTANTLIIHNDEVVVNEPSIVALNRNNPKEVLAVGKKALMMHEKTHESIRTVRP
                     LKDGVIADFNAAELMIRELIKLVYPKKPLFPPSWRMMICIPSSITEVEKRAVRDSAEQ
                     AGAKEVYLIHEPMAAALGIGIDVEEPVGNMIIDIGGGTTGITVIALAGIVCDQSIRIA
                     GDEFTADIMEALRRYHSLLIGERTAEQIKIQVGAAMKEIDNPPDDFPVNGRDLVTGIP
                     KQIMVSYQEIAEALDKSIFKIEEAILKALEQTPPELAADIYRRGLYITGGGALLRGLD
                     KRLSNKIKLPVHVADDPLKSVVRGTGIALKNYDRYPFVMR"
     gene            2067..3266
                     /locus_tag="PK977_12930"
     CDS             2067..3266
                     /locus_tag="PK977_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF19070.1"
                     /translation="MRIINRTVLILSLVSLFADVASEMLYPVIPVYLKEIGFSILLIG
                     ILEGVVNFTAGISKGYFGKLSDEKGLRLPFVKLGYLFSAVSKPLMAVFVYPAWIFFVR
                     SIDRLGKGIRTASRDALLSQQATKETKARVFGFHRSMDTVGASIGPVIALLFLYFSPG
                     SYTTLFYIAFIPGIISVLLIFLLKEKKQPVSTLGKGNFFSYFSYWKIASPEFKKIVPV
                     LLLFALFNSSDVFLLLITKESIGSSNVTIMGATFNSDTITIAAYIFYNLIYALSSYPI
                     GVMADRTGFRKMIASGLILFALVYTGFAFNPSVSVIFLLFAVYGVYAAATEGVVKAWI
                     TNLSHEQNTATAIGFYTSAESVCSLFASIIAGAIWTGIGSTYTFLISGLVAFVVAIYF
                     TLIFRNK"
     gene            3356..3823
                     /locus_tag="PK977_12935"
     CDS             3356..3823
                     /locus_tag="PK977_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19071.1"
                     /translation="MRILCLNVVFILLVSSLQAQSKSSGSSGYQTALGVKVWDGGGIS
                     LKHFVNGNNALEFIGYFWRQGVRITGLYEIHGPINGAAGLQWYIGPGAHIGFYNTRNG
                     DRSFIGIDGVLGLDYKFKGAPINMSLDWQPSFEFGDNRGFVGSWGGLGIRYTF"
     gene            complement(3881..4872)
                     /locus_tag="PK977_12940"
                     /pseudo
     CDS             complement(3881..4872)
                     /locus_tag="PK977_12940"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
     gene            complement(4922..>5262)
                     /locus_tag="PK977_12945"
     CDS             complement(4922..>5262)
                     /locus_tag="PK977_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRF19072.1"
                     /translation="LLKAAANQQLDKVNVDTDPRTAVTIVAVSGGYPGDYKKGIEIKG
                     LSDINPEDSILFHMGTAEQAGKIVTSGGRVFCITSYGRSVFDAAEISKEELGKVSFTG
                     MEYRSDIGFD"
     assembly_gap    5263..5304
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5305..6198)
                     /gene="purD"
                     /locus_tag="PK977_12950"
     CDS             complement(<5305..6198)
                     /gene="purD"
                     /locus_tag="PK977_12950"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRF19073.1"
                     /translation="MRILLLGSGGRENALAWKIAQSTHCEKLFIAPGNPGTAKYGTNL
                     PISLLDFEAIKKACINEKIDLVVVGPEEPLVKGITDYLISTPALKNLDVIGPGKNGAQ
                     LEGSKAFAKAFMMRHNIPTAAYKEFTLGNYAEGVQYLQQHPLPIVLKADGLAAGKGVV
                     ICENHLEAIAEFELMIQRAKFGEAGKKVVVEQFLKGIEMSVFVLTDGKHYVILPEAKD
                     YKRVGEGDTGPNTGGMGAISPVPFADDQLMKKIEEKVIKPTVDGLQKEGLEYRGFIFL
                     GLMISGDEPYMIEYNCRMGDPE"
BASE COUNT         1573 a         1375 c         1229 g         1897 t
ORIGIN      
        1 tcaacacata aaaattggtg cttttgtctt ttatcacctc atcaatatat cctaccatat
       61 gaccgggtgg gaaattaaaa gaataagtac ttgtgagcac tgtatctcct ttgttcacct
      121 ctacactttg aggtataccc cttaacgtca ggtagcgggg atctttacca tcccattcca
      181 cggtcccgaa gttgccactt tttttcagcg aagcatttac cttatgctga acatgcaaca
      241 agctcattgc ctggctgaag ttagggctaa cattgaccac tacccctacc gcactgccat
      301 cagaacccag tacggccata ttgtctttta tccccnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtagcc gggcgggaaa
      421 aatagcggcg gtaatgcccc aatgtgtcat aaggtacgga atcctgcact gctgaaactg
      481 ttgtatccct tcttccatag tgagaaggca acaggttcag caaagaatca ttcaggcgat
      541 ttacccgctt attctcttcc cggagagtga aatagtcctc taccttatta tactgtgagt
      601 tgatgacacc tgtcacttca ttggctactc caaggaattt agcccggtga aagcggttat
      661 agctgaataa aaaccataac gcaactccct gtaaaataag gaaagcgaag aaagttctgt
      721 accggcgaat aaaaagaagg acgttacgca taaggtgnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnataacccg agattgcagt tgaaaaaatt
      841 caattttatc tcattacaaa aggataccgg tcataatttt tcagagcaat tcctgttccc
      901 cttaccacac tcttcagcgg atcgtcagcc acatgaacag gcaatttgat cttattgctg
      961 agtcgcttgt cgagccccct taataaggca ccacctccgg taatgtacaa tcccctgcga
     1021 tatatatcag cggccagctc aggcggtgtt tgctccagtg cctttagaat tgcttcttca
     1081 attttaaaaa tgcttttatc caatgcttct gcaatttcct ggtagctcac catgatttgc
     1141 ttgggaatac cggttaccag gtcacggcca tttacgggaa aatcatccgg cggattgtca
     1201 atctccttca tagcagcgcc tacttgtatc ttgatctgct cggccgtacg ttcaccaatc
     1261 agcaaactat ggtaacggcg caaagcttcc ataatatcag cggtaaattc atccccggcg
     1321 atacggatag actggtcgca gacaataccg gctaaggcaa taacagtaat acctgtagtt
     1381 ccaccaccaa tatcaatgat catgttacca accggctctt ctacatcgat acctataccc
     1441 aatgcagcag ccataggctc atgaataaga tatacttctt tcgcccctgc ctgctccgca
     1501 ctgtcacgaa cagccctttt ttccacttca gtaattgaag aaggaataca gatcatcatc
     1561 cgccagctgg ggggaaacag gggttttttg gggtatacca atttgatcag ttcccttatc
     1621 atcaattcgg cggcattgaa atcggcaatt acaccgtctt tcaacggccg aaccgtacgg
     1681 atactttcgt gggttttttc atgcatcatc agcgcctttt taccaaccgc cagcacttct
     1741 ttcgggttgt tgcggttcag cgctacaatg ctcggttcgt tcaccactac ttcgtcgttg
     1801 tgtataatga gggtattggc tgtacccagg tcgatggcta tttcttgtgt aaaccagtta
     1861 aaaagtccca ttaatatttc cttggatttt gtaggggcaa aggtggagga aataatttga
     1921 attaacaaag cggaaaccct tgtctggcaa agatttttta cgattttcat gactcagaaa
     1981 ttctggttaa aatatttttt ttaatagcat aaccccgaaa ttttcagtaa tagtttcagg
     2041 acaatttttt cttctacctt catcacatgc gaatcataaa ccggactgtt cttattcttt
     2101 cgctggtgag tttgtttgct gatgtagcaa gtgaaatgct gtacccggtc atccctgttt
     2161 acctgaaaga aattggcttc agcattttac tgatcgggat actggaaggt gttgtgaatt
     2221 tcacagcagg tatcagcaaa ggctattttg gcaaactgag tgatgaaaag ggtttgcggt
     2281 taccttttgt aaagctgggt tatttgttca gtgctgtttc aaaacccctg atggccgttt
     2341 ttgtatatcc tgcctggata ttttttgttc gctccattga ccggcttgga aaaggtatca
     2401 ggaccgcctc aagagacgct ttgctttcac aacaggcaac caaagagacc aaggccaggg
     2461 tttttggatt tcaccgaagt atggatactg taggtgcatc catcggacct gtcattgcat
     2521 tgctgttttt atacttcagt cccggaagct atacaacttt attctacatt gcctttattc
     2581 cgggcatcat ctcggtgcta ttaattttcc tgctgaaaga aaagaagcaa ccagtttcta
     2641 ctttgggcaa aggcaatttt ttttcttatt tcagctactg gaaaattgct tcaccggaat
     2701 ttaaaaaaat tgttccggta ttattgcttt ttgccttatt caacagttct gatgtttttc
     2761 tgttacttat cacaaaagaa agtatcggca gcagcaatgt aactatcatg ggagctacat
     2821 ttaattcgga taccatcacc atagctgcct atatttttta caatctcatt tatgccctga
     2881 gttcttaccc tatcggagtt atggccgacc gaaccgggtt tcggaaaatg attgcatccg
     2941 gtcttattct ttttgccctg gtctatactg gttttgcatt taatccttcc gtctcagtaa
     3001 ttttcttgtt gttcgctgtt tatggcgttt atgcagccgc caccgaagga gtagtgaaag
     3061 catggattac taatctgtca cacgagcaaa ataccgctac ggctattggt ttttatactt
     3121 ctgcagaaag tgtatgttcc ctgtttgcca gtatcattgc cggggctata tggacaggta
     3181 ttggcagtac ttatacattc ctcatttccg gtttagtagc ctttgttgtt gccatttatt
     3241 ttacactgat tttcaggaac aagtaatagt taaatctgaa atcttcgttg ttattccttt
     3301 tttggcacag aaatggaaag ggtggcgtgg cgaatcttcc gcaaaattta cagatatgag
     3361 aatactgtgt cttaatgttg tattcattct tttggtttct tccttacagg cccaaagcaa
     3421 aagctccggc agttcaggct atcaaacagc attaggtgta aaagtgtggg atggcggtgg
     3481 catttcactc aaacactttg taaatggtaa taatgcactg gaatttattg gatacttctg
     3541 gcggcaaggc gtaaggataa ccgggcttta cgagatccat ggtccgatta acggtgccgc
     3601 gggattacaa tggtatattg gccccggcgc acatattggt ttttataaca cccgaaacgg
     3661 agatcgcagt tttatcggaa ttgatggtgt attgggtctt gattacaaat tcaaaggtgc
     3721 cccgattaat atgtcattag actggcagcc ttcatttgaa tttggagata acaggggttt
     3781 tgttggcagt tggggcggac taggtattcg gtatactttt tagtgcgatt atcacacata
     3841 cagggaaaac gggccgcagt cattacggcc ttttctttgc ttatgaaaca aagcccagtt
     3901 ttttttctgt agccgcatcc aatgcaggca gctttcttcg ttcaatcagg aagctgttca
     3961 actgggcaat ttctttaaaa atgtaatgct tatctgctgc agccttcaag taatctttgc
     4021 cggtacttcc gcctgtgccg atacgggtac cgatcatgcg atgcaccata ttcatgtggc
     4081 ggtacctcca tgaactgagt tgttcatcta tttctaaaag gcagtttaat aattgaaagg
     4141 gtaattgtaa cagggggtaa cccctataga gcataataaa caatgctgcc ctgcaggcag
     4201 ccggtgataa ggtgcggctt tcattgccaa ccggtttctc aaatatttct tcaaaagcaa
     4261 caaggttatt tttttccgca gcagccaggc tttcttcata tacgctttta taattctccc
     4321 agaaaccggc atcagcccct tcttttaaaa atggcattct ttcgagccac gcatttaata
     4381 attgcaataa tgattttgcg ccctctgctt ttttaataac atctacatgt tctggtttta
     4441 actgggaagt ataatattct ttaccatgac gatgttcaaa tttcagtccc agcttggctt
     4501 ccaattcctt gaactgccag ctttgaaaac ctgatgcagg ccttagcata tccctgaaat
     4561 caagaaaatc cgtgtcatag tttccatgat atctatctgg tgaaccaaca ccctgagaat
     4621 agtaacacag cgattcaacc ggtgtacaat cgtctgcagt tcgggggagt tatcgttgag
     4681 cgccggtttg ttcatgatag caacaactga atctgcttca tgatgcagtt gtttgaacca
     4741 aagttcatag gcctggtgaa tgatgatgaa taacatttca tcatgtgccg ggagcttgtg
     4801 cttatcgctt tcaggaaatt gtgcattcag gatcttatcc agctgcaggt attcatggta
     4861 atgtacgtcc atagcaggct tcgtggtgag caacgaaagt aactaaatag ccattataat
     4921 atcaatcaaa tccgatatcg ctcctgtatt ccattcctgt aaatgaaacc ttacctaatt
     4981 cttcctttga aatttctgct gcatcaaaaa cagaacggcc atatgaagtg atgcaaaata
     5041 ccctgccgcc actggtaact attttgccgg cctgttcagc agtacccata tgaaataaga
     5101 tgctgtcttc cgggtttata tctgagagtc cttttatttc gatgcctttc ttataatcac
     5161 cgggataacc tccgcttact gccacaatgg ttacggctgt tctgggatct gtatctacat
     5221 ttaccttatc cagctgttga ttagctgcag cctttagtaa tannnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnttcagg atcgcccatt cgacagttgt attcgatcat
     5341 atagggttcg tcccctgaga tcatcaaacc taaaaagatg aatcccctgt actccaaacc
     5401 ctctttttgt aatccatcaa cggttggctt aattactttc tcctcgattt ttttcattaa
     5461 ctgatcatct gcaaacggta ccgggctgat agcccccatg ccgccggtat tcggtccggt
     5521 atctccttca cccactcttt tataatcctt ggcctcaggt aaaatgacat aatgctttcc
     5581 gtcagtcaat acaaaaacag acatttcaat acctttcaga aattgttcca ccactacctt
     5641 cttacctgct tcaccgaact ttgcccgctg gatcattaat tcaaattcag caatagcctc
     5701 taaatgattc tcgcagatca ctacgccttt tccggcagcc agaccgtctg cttttaatac
     5761 aatcggcaaa ggatgctgct gcagatattg aaccccttcc gcatagttac ccagtgtgaa
     5821 ttctttgtat gctgcagtag gaatattatg ccgcatcata aaggccttgg caaaggcctt
     5881 actcccttcc agttgtgcac cattctttcc gggtccgatt acatcgaggt tttttagtgc
     5941 tggtgtactt attaaataat cagtaatgcc tttaacgagt ggttcctctg ggcctactac
     6001 taccaggtca atcttttcat ttatgcaggc tttttttatt gcttcaaaat caagtaagga
     6061 aatcgggaga ttggttccgt attttgccgt gccggggttt ccgggagcga taaacagttt
     6121 ttcacagtgg gtactttggg caattttcca ggccagtgca ttttccctgc caccggagcc
     6181 cagtaataat attcgcatgt tagttagtaa ttgctggatt ttaaacgcct taagaataa
//
LOCUS       DAORLS010000179         6220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6819_length_6220_cov_2.349716, whole genome shotgun sequence.
ACCESSION   DAORLS010000179 DAORLS010000000
VERSION     DAORLS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6819_length_6220_cov_2.349716"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PK977_12955"
     CDS             complement(<1..1120)
                     /locus_tag="PK977_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRF19074.1"
                     /translation="MAELKLPRLLAAAKEFNVGQDTIIDFLIGKGFPKDELKPTSKLS
                     EDMYRALQQEFQSDKAAKMKSDQVDLPKGAVSEAKKKKEEEEILFKKEEKAPAKAKKE
                     VPAPAIEEPKVAAEEEKPAKVKTPKEEQPKEEEVIKLEAPEIEGPKIVKKIDLSAIDS
                     STRPKKIVKAKKVEEAKPEAEETPKAKSKKTKKEEVVEEVAPVKEEVTPAVTEEEQPP
                     VIENIEVEKLTGPKILGKIELPVDSDTRPVKDEKKKRKRIPIEKKDTHREIFINKDAD
                     KKPGGGGGFNRDNRGRTGAGGNRRDNRGRREEKLIDEKEIQEKIRETQAKLAGGGGKG
                     KSLKAKLRREKRQELAEAGDAAEDNKLQVTEFISVSELA"
     gene            complement(1209..2456)
                     /gene="nusA"
                     /locus_tag="PK977_12960"
     CDS             complement(1209..2456)
                     /gene="nusA"
                     /locus_tag="PK977_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRF19075.1"
                     /translation="MASINLIEAFQDFKEAENIDRPTMMKVVEDVFKTLLRKKYGSDD
                     NFDVIVNAEKGDLEIIRRRMIVEDGQVEDPLAQIAYADAVKIEPDFEVGEELYEEIDL
                     LDFGRRAILAAKQTLASRISDLKKNVLAKKYGDRIGEIISAEVYQVWKKEILLLDEEG
                     NELILPKSEQIPQDYFKKGESIRAVVKKVDMKNNNPVIILSRTSPDFLAKLLEIEVPE
                     IFDGLIVIKKIVRDPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INWTSNIQLLIQRSLTPARITSMELDNENKHANVYLKPDQVSLAIGRRGVNIKLASEL
                     TGYELDVYRDNEGEEEGFDIDLDEFSDEIETWVIDELKRVGCDTARSVLDLTAEELER
                     RTDLEKETIAEVRKVLKEEFEKE"
     gene            complement(2520..2990)
                     /locus_tag="PK977_12965"
     CDS             complement(2520..2990)
                     /locus_tag="PK977_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HRF19076.1"
                     /translation="MSTDPQITAIEQKITNLISTEPELFLVEVKIKPTNNIKVYIDGD
                     QGVSIEKLVNYNRKLYKRIEEEGMYPDGDFSLEVSSPGLDEPLKMYRQYQKNIGRFAE
                     VIDKEGQRRDGKLVGVGENEVVLEETRGKGKKQEVVTHTIPFEHIKSTKILIKF"
     gene            3099..3401
                     /locus_tag="PK977_12970"
     CDS             3099..3401
                     /locus_tag="PK977_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19077.1"
                     /translation="MVNKSVKHYLQVKHQPHRYYLYTVKYVLLALLLYIAYQFIFKLV
                     VPVYKTTKQVKQKFREMHSQMEEQQKQQQGYSPGYAEPKTGKKENIGDCIDFEEVK"
     assembly_gap    3419..3430
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3431..>4158)
                     /locus_tag="PK977_12975"
     CDS             complement(<3431..>4158)
                     /locus_tag="PK977_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRF19078.1"
                     /translation="SRSNIHLPGVQEELIRAIHATGKPVIVMINAGRPLVFNWTADNV
                     PAILYNWWLGTEAGNAIADVLFGDYNPSAKLPMTLPRSEGQIPIYYNYYTTGRPAKND
                     SDRFYRSAYVDESIYPKYPFGYGLSYTQFQYSDIRLTKNQYRANDKIEVIITLTNTGN
                     YDGEEVVQLYIRDVAASVVRPVKELKGFQKVSLKKGEAKEVRFMLTADDLRFYNDRLQ
                     FIYGPGEFKLYIGGSSNDVKETTF"
     assembly_gap    4159..4188
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4189..>4561)
                     /locus_tag="PK977_12980"
     CDS             complement(<4189..>4561)
                     /locus_tag="PK977_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRF19079.1"
                     /translation="NPEREQKQLNNPVHRAAARDMAKKSIVLLKNQEQLLPLSKETKS
                     IALIGPFIKAVRDNLGFWSMEWPDDSSTIVTQWQGIKDRVSGLTKLLYAKGCNINDSS
                     KAGFAEAIEAAMQAEVVIMSMG"
     assembly_gap    4562..4581
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4582..5673)
                     /locus_tag="PK977_12985"
     CDS             complement(<4582..5673)
                     /locus_tag="PK977_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF19080.1"
                     /translation="MKLNLSINLLFCFVLAWAVSASAQKISYQQIGKTIDQRVDSVLR
                     LMTLDEKVGQLNQYSGDWSHTGPITKDGDKQNQVRDGQLGSMLNVIGVQHTRELQEMA
                     MQSRLKIPLLFGQDVIHGFKTTFPIPLAEAASWDTAAIRLSARIAATEAAAAGVHWTF
                     APMVDISRDPRWGRVMEGAGEDPFLGSVIAAARVKGFQGNGLGSVDAVMACAKHFAAY
                     GAAVGGRDYNTVDMSLRQLHETYLPPFKAAVDAGAATLMNSFNDLNGIPATGNKYLQR
                     DILKGQWKFSGFVVSDWGSVGEMVNHGYAKDNYEAALLAITAGSDMDMESRSYKDHLA
                     QLVKEGKVKIQLVDDAVKRILRKKFELGLF"
     gene            complement(5685..>6220)
                     /locus_tag="PK977_12990"
     CDS             complement(5685..>6220)
                     /locus_tag="PK977_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRF19081.1"
                     /translation="TSFAELSAFKLSNGEKLPTLREYLLTGLANNQQTRLVLEIKPSG
                     ISKERGKLIAGKVVSLVRELKAEPMIVYISFDYDILKRIIEIDSKAITQYLNGDKSPK
                     ELKTDKINGADYHLSVFQKKTEWIEQAKKNKIELNAWTVNNAEDMQWLLSKGFDYITT
                     NEPELLFEITRTNSRKK"
BASE COUNT         1479 a         1544 c         1080 g         2055 t
ORIGIN      
        1 tagccaattc acttacactg atgaattcgg ttacctgcag cttgttgtct tctgctgcgt
       61 caccagcttc tgccagttcc tgtctttttt cacggcgcaa tttggccttc aggctcttac
      121 cttttccgcc cccgccggcc agtttggcct gcgtttccct gatcttttcc tgtatctctt
      181 tttcatcaat gagtttttct tccctgcgac ctctgttgtc acggcggttg ccccctgcgc
      241 cagtccgtcc tctattatct ctgttgaatc cgccgccacc gccaggtttc ttatcagcat
      301 ctttgtttat aaaaatctct ctatgtgtat cttttttctc aataggaata cgtttcctct
      361 ttttcttttc atcctttacg gggcgggtat cactatctac aggcaattca attttaccca
      421 ggatctttgg accagtcagt ttttctactt caatattctc aattacggga ggctgctctt
      481 cttccgttac tgcgggggta acttcttctt taaccggcgc aacttcttca actacttctt
      541 cctttttggt cttctttgat tttgctttgg gcgtctcttc tgcttccggt tttgcctcct
      601 ctaccttttt cgcttttaca attttcttag gcctggttga agaatcgatt gcagaaagat
      661 caattttctt tacaatcttg ggaccttcaa tttccggggc ttccagtttt attacctctt
      721 cttctttggg ttgctcttct tttggagttt ttacttttgc cggcttctct tcctcagcag
      781 caactttggg ctcttcgatt gctggtgctg gcacttcttt ttttgctttt gcaggcgcct
      841 tctcttcctt tttgaaaagg atttcctctt cttctttctt ctttttggct tcagaaacag
      901 cgcctttagg cagatccacc tggtcgcttt tcatttttgc tgccttatca ctctggaatt
      961 cctgctgcaa agcccggtac atgtcctcag atagtttcga agtgggcttc agttcatctt
     1021 taggaaatcc tttccctatc aggaaatcaa taatggtatc ctgtccaacg ttgaactctt
     1081 tagctgccgc taataatctc ggaagtttta attctgccat tcaaaagttt taaagtcaat
     1141 agtgaatggt caatagtgaa taggaaaagt catgttgaat gactattcac tattcagcat
     1201 tcaccttatt attctttttc aaattcttct ttcaatactt ttctgacctc agcaattgtt
     1261 tctttttcaa ggtcagttct tctttccagt tcttcagctg tcagatccag cacactgcgg
     1321 gctgtatcac aacctacccg cttcaattca tcaataaccc atgtttctat ttcatcgctg
     1381 aattcatcca ggtcaatatc aaatccttct tcttcacctt cgttatcacg gtaaacatcg
     1441 agttcataac cggtcagttc tgaagcaagt ttaatattta caccacgtct gccaatcgcc
     1501 aacgaaacct ggtcaggctt caggtacaca ttggcatgtt tgttttcatt atccagctcc
     1561 atgctggtga tacgggcagg agttaatgaa cgctgtatca gcaactgaat attactggtc
     1621 cagttgatca catcaatatt ctcgttcttc aattctctta cgatgccgtg aatacggctt
     1681 cccttcatac ccacacaggc gcctacgggg tcaatgcggt catcgtaaga ttctacggct
     1741 accttagccc tttctcccgg atctcttaca atctttttga taacaatcag gccatcaaat
     1801 atttccggca cttcaatttc gagcaattta gcaagaaaat caggggaagt acgggaaagt
     1861 ataatcaccg gattattatt tttcatgtct acctttttca ccacggccct gatagattcg
     1921 cccttcttga aataatcctg cgggatctgc tcactcttgg gcaatatcaa ttcatttcct
     1981 tcttcatcga gcagtaatat ttctttcttc caaacctggt acacttctgc gctaatgatc
     2041 tcaccgatcc tgtcgccata tttcttggcc agtacatttt tcttcagatc gctgatacgg
     2101 ctggccaggg tttgcttggc agccaggata gccctgcggc caaagtcaag caggtcaatt
     2161 tcttcatata attcctcacc tacttcaaaa tcaggctcga tcttcaccgc atctgcgtag
     2221 gcaatttgtg ccagcgggtc ttctacctgt ccatcttcca caatcatccg gcggcggatg
     2281 atctccaggt cacctttttc agcatttacg atcacatcaa aattgtcatc gctgccatat
     2341 tttttacgaa gcagtgtttt gaaaacgtct tccaccactt tcatcatggt aggacggtca
     2401 atattttcgg cttctttaaa gtcctggaac gcctcaatca ggttgatact tgccatagca
     2461 aaacaatttt cgatttttga ctgcagattt cagatttatt gtttgcaaca aacccgcaat
     2521 cagaatttga ttagaatttt agttgatttt atatgctcaa aaggaatggt atgtgtaacc
     2581 acttcctgtt ttttgccctt gcccctggtt tcctccagta ctacctcgtt ttcaccaaca
     2641 cccaccagct ttccgtccct tctttgtcct tccttatcta ttacttccgc aaaacggcca
     2701 atattttttt gatactggcg atacattttt agcggttcgt caaggccggg tgaagacact
     2761 tccagtgaga aatcaccatc aggatacatt ccctcctctt cgatccgttt gtatagtttt
     2821 cggttataat tcaccagttt ttcaatgcta acaccttgat caccatcaat ataaaccttt
     2881 atattgttgg taggcttgat ttttacctct accaaaaaaa gctccggttc tgtactgatc
     2941 aggttcgtga tcttttgttc aattgccgtt atttgtgggt ctgtactcat gttatataaa
     3001 agaagaaggg aacggcctcg ttcccttctt tcgtctattt ccgctgcaaa tgtaaggcat
     3061 ggcgttattc cagccaaatt cctgttgcaa aaaaccgcat ggttaataaa tcggtaaagc
     3121 attacctgca ggttaagcat caacctcacc gttattatct ttacaccgtg aagtacgttt
     3181 tattagccct cctgttgtat atcgcttacc agttcatttt taaattggtg gttcctgtgt
     3241 acaaaactac caaacaggta aagcaaaaat tccgtgaaat gcactctcag atggaagaac
     3301 agcaaaaaca gcaacaaggc tattcaccgg gttatgctga acctaaaaca ggaaaaaaag
     3361 aaaatatcgg ggattgcatc gactttgaag aggtgaaata agtttttatt tctttagtnn
     3421 nnnnnnnnnn aaaggtcgtt tctttcacat cattgctgct gcctccaatg taaagcttga
     3481 attcgcccgg cccataaatg aattgaagac ggtcattata aaaacgaagg tcatcagctg
     3541 tcagcatgaa acgaacttct tttgcttctc cttttttcag agaaactttt tgaaatccct
     3601 ttaattcttt cacaggccgt actacggaag cagcaacatc tcttatatat agctgaacca
     3661 cttcctctcc gtcataattc ccggtattcg ttaaggttat tatgacttca attttatcat
     3721 tggcccggta ttgattcttt gttaagcgta tatcgctgta ctgaaactgt gtatagctta
     3781 atccataccc aaatggatat tttggataaa tagattcatc tacataagct gaacgataaa
     3841 acctgtcgct gtcattctta gccggacgac ctgtagtata atagttatag taaatgggaa
     3901 tctgcccttc acttctggga agcgtcatgg gaagtttggc tgaaggatta taatcgccaa
     3961 acaaaacatc tgcaatagca ttaccagctt ccgttcccaa ccaccagttg tacaggatcg
     4021 ccggtacatt gtctgccgtc cagttaaata ccaaaggccg gccggcgttt atcatcacaa
     4081 ttactggctt gcccgttgca tgaattgccc ttattagctc ttcctgcacc cccggtaaat
     4141 gaatattact cctgctttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc catactcata
     4201 atcactactt ccgcctgcat ggctgcttca attgcttcgg caaaacctgc ttttgaactg
     4261 tcattaatat tacagccttt tgcatataat aattttgtta atccactaac acggtcttta
     4321 atgccctgcc actgcgtaac aatagtggag gaatcatccg gccattccat gctccagaaa
     4381 cccaggttat ccctcacggc tttgatgaaa gggccgatca aagcaatgga ctttgtttct
     4441 ttgctgagtg gcaacagttg ttcctgattt ttcagcaaca caatactttt ctttgccata
     4501 tccctggcgg cagccctatg aaccggattg ttcagttgct tttgctccct ttcaggatta
     4561 cnnnnnnnnn nnnnnnnnnn naaacaaacc cagttcgaat ttctttctta gaattctttt
     4621 cacggcatca tctaccagct gtatttttac tttgccttct ttcaccagtt gagccaggtg
     4681 atctttgtaa ctccggcttt ccatatccat atcactgccg gcggtaatag ccagcaatgc
     4741 tgcttcataa ttatcttttg catagccgtg atttaccatt tctccaacag agccccagtc
     4801 actcactaca aacccgctga atttccattg gccttttaaa atatcccgtt gtaagtattt
     4861 atttcccgtt gccggaattc cattcagatc attaaacgag ttcatcaggg ttgctgcacc
     4921 tgcatcaaca gccgctttaa aaggaggtaa atacgtttca tgtaactgac gtaaactcat
     4981 gtcaacagtg ttatagtccc ttccgcccac agctgcccca tacgctgcaa aatgtttagc
     5041 acaagccatg acggcatcaa cacttcccaa tccatttccc tggaaacctt tcactcttgc
     5101 tgctgcgatt acagatccta aaaacggatc ctcaccggcg ccttccatta ctcttcccca
     5161 tcgcggatcc cgggaaatat ctaccattgg tgcaaatgtc cagtgcacac cggctgccgc
     5221 tgcttctgta gccgctatcc tcgcagacaa acgaatggct gcggtatccc agctggcagc
     5281 ttcggccagt ggaataggaa aagttgtttt gaatccatga atcacatcct gcccgaacaa
     5341 cagtggaatc tttaatctag actgcatggc catttcctgc aactctcttg tatgctgcac
     5401 accaattaca ttcagcatgg agccaagctg accatctctc acctgatttt gtttatcacc
     5461 gtctttggtt atcggcccgg tatgcgacca gtcaccactg tattggttta gctgtccgac
     5521 tttttcatcc agtgtcatca gcctcagtac tgaatcaacc ctctggtcaa tagtttttcc
     5581 gatttgttgg taagaaattt tttgtgcact ggcagagaca gcccaggcaa gcacaaagca
     5641 aaacaacaaa ttgattgaaa gattcagctt cattatttcg gtacttattt cttccttgag
     5701 tttgttctgg ttatttcaaa aagcaattcc ggctcgtttg tagtgatata atcaaatcct
     5761 tttgacagca gccattgcat atcttctgca ttatttaccg tccacgcatt caactcaatt
     5821 ttatttttct tagcctgttc gatccattcc gtttttttct gaaaaacgga caaatgataa
     5881 tccgcaccat tgattttatc cgtcttcagt tctttgggtg atttatcccc attaaggtat
     5941 tgtgttatgg ctttactatc aatctcaata attctcttca ggatatcata atcaaaactg
     6001 atatatacaa tcatcggttc tgccttcaat tcccttacaa gactgactac tttcccggca
     6061 atcagctttc ccctttcctt gctaattccg gatggtttga tctccaatac cagccttgtc
     6121 tgctgattat tggctaaccc cgtcaataaa tattcccgga gggtaggcag cttttcgcca
     6181 ttagaaagtt tgaatgctga taattcagca aaagaggtct
//
LOCUS       DAORLS010000180         6212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6834_length_6212_cov_1.622543, whole genome shotgun sequence.
ACCESSION   DAORLS010000180 DAORLS010000000
VERSION     DAORLS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6834_length_6212_cov_1.622543"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..403)
                     /locus_tag="PK977_12995"
     CDS             complement(158..403)
                     /locus_tag="PK977_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19082.1"
                     /translation="MARSHHRKKHKEHVRQYRNTKDELVTEPKGKTFAVFTVIGVLTG
                     MAIGYFATESSLLWMGVGAVAVGIIGYYIGRKIDGDA"
     gene            538..1014
                     /locus_tag="PK977_13000"
     CDS             538..1014
                     /locus_tag="PK977_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HRF19083.1"
                     /translation="MKNSIIATFIAAFTLVACSKSSVDENSNSNNSGTATAIPSSNVP
                     EAVRNSFNSSFSGATEVEWLRGSNNFISQFNLSNQRHDARFDDNGHRSSHSVISLAAV
                     PAAVLEAFRQRFPTDNVYEWKLRNDGTWKAHFMRGSVKYEATYSAEGSLLKFEQAS"
     gene            complement(1078..1437)
                     /locus_tag="PK977_13005"
     CDS             complement(1078..1437)
                     /locus_tag="PK977_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRF19084.1"
                     /translation="MKLFVAGLPYDMDDAELEEFFEKFGTVTSVKVAIDRETGKSKGF
                     AFVEMPLEAEAREAIEGLNNLSIGRKTLVVKQAEDKPGGGGYGGSGGGGGYRGGGNRG
                     SGGGYNKYGNDRDRRRY"
     gene            1592..>2372
                     /locus_tag="PK977_13010"
     CDS             1592..>2372
                     /locus_tag="PK977_13010"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRF19085.1"
                     /translation="MKEVYIISAVRTPMGSFGGSLKSLSAAQLGAVAIKGALAKAGVK
                     PDQVQDVLMGCVMQANLGQAPARQAAKFAGLPNEVNCTTVNKVCASGMKAIAQAAQSI
                     LLGDADIIIAGGMESMSNVPFYADSVRWGNKYGNVSLIDGLAKDGLTDVYDGKAMGNA
                     AELCASTCGISRSDQDAFAIESYKRSQAAWEKGLFDNEVVPVEIPQRKGDPVVFAKDE
                     EPWNVKFDKIPTLNPAFIKDGTVTAANASTMNDGAAALVLMS"
     assembly_gap    2373..2391
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2392..>2668
                     /locus_tag="PK977_13015"
     CDS             <2392..>2668
                     /locus_tag="PK977_13015"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217866.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRF19086.1"
                     /translation="KADELGLKPIAKIRSYADAEQAPEWFTTTPSLAVPKAVAKAGLK
                     MEDITYWELNEAFAIVGIENSKRMKLDPARVNVHGGAVSLGHPLGCSG"
     assembly_gap    2669..2825
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2826..3329
                     /locus_tag="PK977_13020"
     CDS             <2826..3329
                     /locus_tag="PK977_13020"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF19087.1"
                     /translation="EDDAALIADLSRRTFYETFAAQNTKEDMEKFMEEQFSRELLMEQ
                     VCEPGNTFLLAFVEDLPAGYARLKESENPSSLGHVDAMEISRIYVVNTAISTGIGKAL
                     MEECIQVAIQKNKEVIWLGVWEHNTRAISFYKKFGFIQFGTHLFMLGDDAQTDWLMKK
                     ELINLEK"
     assembly_gap    3414..3503
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3504..4206
                     /locus_tag="PK977_13025"
     CDS             <3504..4206
                     /locus_tag="PK977_13025"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRF19088.1"
                     /translation="FGEYHNNAIAHWLQLEVTKDLKQSRNLVLGAEMFEADNQEPLDL
                     YLQGKLSAKGLDSNARLWKNYPTDYAPLVNFAKENKLEFAATNIPRKFASLVSKGGFE
                     KLDSLSTKEKSWIAPLPIAYDAELPGYKKMLEMMGGHGGPNLPKAQAIKDATMAHFIL
                     QYYKSGSLFIHYNGAYHSDNYEGILWYLKRQRPELRYATISTVSQKDIHSLEAVNKGK
                     ADFIICVDEDMTNTY"
     gene            4283..4888
                     /locus_tag="PK977_13030"
     CDS             4283..4888
                     /locus_tag="PK977_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19089.1"
                     /translation="MSLSRTIFIFLYLLLSPAVFFAQQKDVSIRIVQDDEAHQLNDFE
                     TRLALEKEGFKIQVLLSKTEGVYVFASFSDSVYKTGPSDPVPGFKDLPNLAMAEEEFN
                     NNKELIISNEGWSYWFYDPELNWHRFNKKLVFLDSNKVVGVKSIKQYYLLDKKEEVKV
                     KDNNRPLYIFFVAVSETDDKGRPVKEFMRRKLKIEWKAEDD"
     assembly_gap    5109..5118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5119..5551
                     /locus_tag="PK977_13035"
     CDS             <5119..5551
                     /locus_tag="PK977_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19090.1"
                     /translation="VMLQNVQGVYVFASIRDSIYRFTETSPIRDFPYLPLLQLREDEF
                     NENKEMNISETGWSYWFYDTAYEWHPFNHKLTRIDKDRIVGTKNIRQFFDVNEGRVIK
                     IREINTPLYLFFVAVEEYDKDGKPLKELVRRKVKVEWKNEE"
     gene            5732..>6212
                     /locus_tag="PK977_13040"
     CDS             5732..>6212
                     /locus_tag="PK977_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19091.1"
                     /translation="MKARILYGAVLLTFFSLSFIACQKDQSDNSDYTMETARHSDDQS
                     RFSNEVDAAANDVNITVESAAGFAGRIENIQSFICDATVAVDTMSNPRTITITYNGTN
                     CLGNRIRTGVIVVSMAQVVRWKNAGAVINVTYQNFKVTRLSDNKSITLNGTQSFTNVS
                     "
BASE COUNT         1765 a         1176 c         1323 g         1672 t
ORIGIN      
        1 aaaaaacagt aaataccggt aaggaaaaat ggacttcagt tgtgaaggat aatcgttttg
       61 aaaagaaaga taacctgact ggtacaatta aattgaaacc ctacagctgc actattctct
      121 atcaataaaa ctaaataaca aacaagtata ttagctgtca ggcatcacca tcaatttttc
      181 tgccgatata gtagccgatt atgccaactg ctactgcacc gacccccatc cacaataatg
      241 aactttcggt ggcgaaataa ccgattgcca taccggttaa tacaccaatg acggtaaata
      301 cggcaaaggt ttttcctttt ggttcggtta ccaattcatc ctttgtattc cggtactggc
      361 gaacatgctc cttatgtttc ttgcggtgat gtgatctggc cataaacgta ggtttctata
      421 aaattagaag gtcattttct tcattacaaa tgttttttat taaaaccggt tacatcatga
      481 cttccggcat ttacctactt gatgtatatc atttcctcat ttaaaattaa atagcacatg
      541 aaaaacagca tcattgccac atttattgca gcattcacat tggttgcctg cagcaaatca
      601 tctgtagatg aaaacagtaa cagtaataat tccgggacag ccactgccat tccttcttcc
      661 aatgtgccgg aagcagtaag aaattccttc aatagcagtt tcagcggtgc cacagaagtg
      721 gagtggttgc ggggtagcaa taattttatc agccagttca acctttctaa ccagcggcat
      781 gatgcccggt ttgatgacaa cgggcaccgt tcttcccatt cagtaatttc attggctgcc
      841 gtaccggctg ctgttctgga ggcctttcgt caacgttttc ctacggataa tgtatatgag
      901 tggaaattga gaaatgatgg cacctggaaa gcacatttta tgagagggtc agttaagtat
      961 gaagctactt atagcgctga aggcagtttg ctgaaatttg aacaagccag ttaggtctct
     1021 gcttataaaa caaacagtcc gccgattggc ggactgtttc ataatttaat gtataggtta
     1081 atatctgcgt ctgtccctgt catttccgta tttattataa ccaccgccac tacctctgtt
     1141 tccgccaccc cggtagccac caccgccacc gcttccacca tatccaccac cacctggttt
     1201 atcttcagct tgttttacta ccaaagtttt ccggccgata gagaggttgt tcaggccttc
     1261 aattgcttcc cgggcttctg cttcgagggg catttcaaca aatgcaaatc ccttactctt
     1321 gccagtttcc ctgtcaatag ccacttttac agaggtcact gtgccaaatt tttcgaaaaa
     1381 ttcttccagc tctgcatcat ccatatcata gggcaggccg gcaacaaaaa gcttcatttc
     1441 aaattttttg taaaagtaat tgcttctggc cgtaatcagg caggattgat ctgatattta
     1501 ttaacctgta tatcaacctg tcagtagcag caacactaac aatcgttaac tttggcaccc
     1561 aaacctgatt tttcaatttc aaacaagccg tatgaaggaa gtatatatta tctcagctgt
     1621 ccgcaccccg atgggaagtt ttggaggttc attaaagagt ttatcagctg ctcagttggg
     1681 ggctgtggcc atcaaaggag ccctggcaaa agccggagta aaacccgatc aggtacagga
     1741 tgtattgatg ggatgtgtga tgcaggccaa cctgggccag gcccctgcaa ggcaggcggc
     1801 taagtttgcc ggattaccaa acgaggtgaa ttgtaccaca gttaataaag tttgtgccag
     1861 cggtatgaaa gccattgcac aggctgcaca aagtatttta ttaggagatg ccgatatcat
     1921 tatagccggt ggtatggaaa gtatgagtaa tgttcctttt tatgctgata gtgtgagatg
     1981 gggcaataaa tatgggaatg tctcattaat tgacggattg gccaaagatg gccttactga
     2041 tgtatatgac ggaaaagcca tgggcaatgc cgcagaacta tgtgcctcca cctgtggtat
     2101 cagccggtca gatcaggatg cttttgccat tgaaagctat aaacgttcgc aggcggcatg
     2161 ggaaaaaggt ttatttgaca atgaggtagt gccggtagaa attccccaac gtaagggtga
     2221 tcctgttgtg tttgccaaag acgaagaacc ctggaatgta aaattcgata agataccaac
     2281 attaaatccg gcctttatca aagatggcac tgttacggct gccaacgctt ccacgatgaa
     2341 tgacggtgct gccgcattag tgctgatgag cannnnnnnn nnnnnnnnnn naaagcggat
     2401 gaactggggc tgaaacccat tgcgaagatc aggagctatg cggatgccga gcaagccccg
     2461 gaatggttta ctaccactcc ctcgctggct gtgccgaaag ctgttgccaa ggcaggattg
     2521 aaaatggagg atattactta ttgggaattg aatgaagctt ttgccattgt aggtatcgaa
     2581 aacagcaaac gtatgaaact tgatcctgcc agggtgaatg tccacggcgg tgctgtttca
     2641 ttagggcatc cgttgggttg cagcggtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnngaaga tgatgcagca ctgatagcag acctcagccg ccggacattt tatgaaacct
     2881 ttgcggctca aaacacaaag gaagatatgg aaaagttcat ggaagagcaa ttcagccgtg
     2941 aattattgat ggaacaggtg tgtgaaccgg gtaatacttt tttacttgcc tttgtggaag
     3001 atctgcccgc tggttatgca agattgaaag agtccgaaaa cccttcatcg ttaggccatg
     3061 tggatgcaat ggagatttca agaatatatg tggtgaacac agcgataagc acaggtatcg
     3121 gtaaagcact gatggaagaa tgtattcagg ttgccataca aaagaataaa gaggtgattt
     3181 ggctgggagt gtgggagcat aatacccgtg ccatttcatt ttataaaaaa tttgggttca
     3241 tccaatttgg cactcatttg tttatgctgg gtgatgatgc acagacggac tggctgatga
     3301 aaaaagagct aataaactta gaaaagtaag aacatgaaaa aattactgct tttaacagct
     3361 tttgtactct ctcatctggt aagtaataca caagataaaa gcccgtatgt gctnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnngtttggc gagtatcata acaatgccat tgcgcactgg
     3541 ctgcagttag aagtaacaaa agaccttaaa caaagccgta accttgtgct gggagctgaa
     3601 atgtttgaag ctgataacca ggagcccctg gatttgtacc tgcaaggcaa attaagtgcc
     3661 aaaggacttg attccaatgc ccgtttgtgg aaaaactacc cgacagatta tgcaccgctg
     3721 gtgaactttg ctaaggagaa caaactggaa tttgccgcca ccaatatccc ccgcaaattt
     3781 gcttcgctgg tttcaaaagg aggctttgaa aaattggaca gtctttcaac caaagaaaaa
     3841 agctggattg cgccgctccc tatcgcttat gatgccgaat tgccggggta taaaaaaatg
     3901 ctggagatga tgggcgggca tggcgggcca aatttgccaa aggcccaggc cattaaagat
     3961 gctacaatgg ctcatttcat tttacagtac tacaaaagtg ggagtctgtt tattcattac
     4021 aacggggctt atcattccga taactacgag ggcatactct ggtacctgaa acggcaaaga
     4081 ccagaactgc gctatgccac tatcagcacg gtgtcgcaaa aggacattca ttcgctggag
     4141 gcggtcaata aaggcaaagc tgatttcata atttgtgtgg atgaagatat gaccaatacg
     4201 tactagattt ctacacaatc ccgttcggat aaagtttctt aggcaattaa acattaattt
     4261 gtgctcaaag agcatttgtt ctatgtcttt atcccggaca attttcatct ttttatactt
     4321 gctgctttca ccggcagttt tttttgcaca gcaaaaagat gtttccataa ggatagtaca
     4381 ggatgatgaa gcacaccagt taaatgattt tgaaacccgc ctggccctgg aaaaagaagg
     4441 attcaaaata caggtgttgt tatccaaaac agaaggggta tatgtttttg ccagtttcag
     4501 tgattctgtt tacaaaacag gtccttcaga tcctgtacct gggtttaaag atcttccaaa
     4561 cctggccatg gctgaagaag agttcaataa taacaaagaa ctgatcatca gcaatgaggg
     4621 ttggtcctac tggttttatg acccggaact aaactggcac cggttcaata aaaaattagt
     4681 gtttcttgac agtaataaag tggtaggggt aaagagtatc aaacaatatt atcttttgga
     4741 taaaaaagaa gaagttaaag tgaaggataa taacaggcct ttgtatattt tctttgtagc
     4801 tgtatcagaa accgatgata aaggaaggcc ggtaaaggaa tttatgcgta gaaaattgaa
     4861 gatcgaatgg aaagccgaag atgactgagt gcctaaaaaa gtagatatca taattgctat
     4921 taatttcata ttttgagaat aatgagatca tattttaaaa aggctgcatt aattgttagt
     4981 ttgattacca tacatggttt tctttttgca caggaaaagg aagttagtat acgtatttct
     5041 caagatgagt cggtgaagct gagtgaattc aatactacag tgcaattgga acgcaaacca
     5101 tttaaaatnn nnnnnnnngg tgatgcttca gaatgtgcaa ggggtttatg tatttgccag
     5161 catccgtgat tctatttacc ggtttacaga aaccagccct atccgtgatt tcccttacct
     5221 gccccttttg caactcaggg aagatgaatt caatgaaaat aaggagatga atatttccga
     5281 gaccggctgg tcatactggt tctatgatac tgcttatgaa tggcacccct tcaatcacaa
     5341 gcttacccgg atcgataaag acaggattgt tggtaccaag aacatccgtc agttctttga
     5401 tgtaaatgaa gggagggtga ttaagatccg ggagatcaat acacccctct atctcttttt
     5461 tgtggcggtg gaagaatacg acaaagatgg taaaccgtta aaagaactgg ttcgtcgtaa
     5521 agtgaaggtg gagtggaaaa atgaggagta acagcatcgt aaattttccg tggtttatcg
     5581 tagtttttct ttttataaca tacactaaat gcgctgctgg ggcgtttaac ttctgtttat
     5641 ttattttacc gggtttattt tttgttgctt taaaccaaat ccctgtcaat gacgtctgaa
     5701 tgatgtcttt tcatttttta aaaccgttta tatgaaagcc aggatcctat atggagctgt
     5761 tttgctcacc tttttttcat tgtcctttat agcctgtcaa aaagatcaat cagataactc
     5821 tgattatacc atggaaacag ccaggcattc tgatgatcag tcaagatttt ccaatgaagt
     5881 ggatgcggca gccaatgatg taaatattac tgttgaatct gcagcaggat ttgcgggcag
     5941 aatagaaaat atccagtcat ttatctgcga tgccaccgta gcggtagata ccatgagtaa
     6001 tccgagaacc attaccatca cttacaatgg caccaattgt ttgggcaacc gcattcgcac
     6061 cggtgtaatt gttgtttcga tggcacaggt tgtccgctgg aagaatgcag gggcagtaat
     6121 aaatgttact taccagaatt ttaaagttac ccgcctgagt gataataaga gcattaccct
     6181 taacggaacg caaagcttta ccaatgtcag cg
//
LOCUS       DAORLS010000181         6189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6863_length_6189_cov_1.664167, whole genome shotgun sequence.
ACCESSION   DAORLS010000181 DAORLS010000000
VERSION     DAORLS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6863_length_6189_cov_1.664167"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PK977_13045"
     CDS             complement(<1..782)
                     /locus_tag="PK977_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRF19092.1"
                     /translation="MTNLSTSQLKTIIITGGAGFIGSHVVRRMVTNYPSYTIVNLDAL
                     TYAGNLENLRDIESAPNYRFEKADILDTAALERIFNQYQPDGVIHLAAESHVDRSILS
                     PLDFVYTNVIGTVNLLNAAKKLWNGNYTGKRFHHVSTDEVFGALGDTGFFTEETKYDP
                     HSPYSASKASSDHFVRAYGDTFGLPYVITNCSNNYGPNHFPEKLIPLFINNIIHKKPL
                     PVYGDGLYTRDWLYVIDHAAAIDLVFHEGKNHSSYNVGGFNE"
     gene            complement(784..1152)
                     /locus_tag="PK977_13050"
     CDS             complement(784..1152)
                     /locus_tag="PK977_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF19093.1"
                     /translation="MYTYPFEKLDVWQLAKQLVVKIYSLTKAFPSDEKFGIVNQMRRA
                     AVSVSSNIAEGSSRNSKKDQANFYGMAYSSLMELLNQLIISNELSWISSEELSIIRKD
                     IEVVSFKINALRKSILKNTN"
     gene            complement(1189..2151)
                     /locus_tag="PK977_13055"
     CDS             complement(1189..2151)
                     /locus_tag="PK977_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF19094.1"
                     /translation="MHILVTGGAGFIGSNLAEALLADDRVSFVTVLDNLATGSLDNVV
                     TFNGHPNFRFIEGDIRNYTDCLNACEGVDLISHQAALGSVPRSINDPLTTNEVNITGT
                     LNIFTAAKEKGIKRIVYAASSSTYGDHPGLPKQEDVIGKPLSPYAVTKYVNELYARVY
                     ADLYGMEFIGLRYFNIFGPRQNPKGPYAAVIPLFAEAILKGVPPTINGDGTHSRDFTY
                     VSNAVQANILSLFTDNAAAINQVYNIACGQQTSLNELFEGLKKEAGSDLQAIHGPERK
                     GDVKHSLADIAKAEKLLGYKVIVSVEEGLSRTFKWYRQVEELRS"
     gene            complement(2173..>3219)
                     /locus_tag="PK977_13060"
     CDS             complement(2173..>3219)
                     /locus_tag="PK977_13060"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRF19095.1"
                     /translation="ILQEKREQTAISRAANVANSQVVENAYVSFKPIKPNRRSIQLMA
                     ILIGLAIPALFVFVSEMLNDKVTTRYDVEKITSAPILGEIGHSYSETVLLVGKTNRSM
                     VAEQFRIIRSNLQYVIGKAEKFVLLITSSYSGEGKSFISTNVGGVMALAGRKTIILEF
                     DIRKPKLISGLNMPKGPGITNFLIGNSALPDLIKLVPGHENLFVLPCGPIPPNPSELL
                     LGDKTDELFDWLRKEFDVVIIDTAPVGMVSDAQTLSKYADATLYIVRQGHTFKKQITL
                     IEEFYQFKKLPKVSVVINDVKMKAGYGYYGYGRYGYGYGYGQDSSYYEEETAPDNFFE
                     RLLHKLSKLNPFRK"
     assembly_gap    3220..3235
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3236..>3940)
                     /locus_tag="PK977_13065"
     CDS             complement(<3236..>3940)
                     /locus_tag="PK977_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19096.1"
                     /translation="VAPKVAGTGILSISMQNTNPNLAADVVNQLMVEYGDYTKELKNQ
                     TSDQTIAFIDSRMEVLGRELDSVQNNLLRYAQDNNLIDAEAQTSAYFDNLSSSDKAIN
                     EQQLQLNTAEYIEIYLRDKKNSYGPVIVPSSLGLNDPTLNELVNAYNTMQIERQQLLD
                     GNVPAENPAVKEASEQIEKLRISLLENLSNIKNSMRKTIASLRQSGGLAEGQLKAMPY
                     KLKGYLELKKQVDNKQA"
     assembly_gap    3941..4130
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4131..>4467)
                     /locus_tag="PK977_13070"
     CDS             complement(<4131..>4467)
                     /locus_tag="PK977_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19097.1"
                     /translation="LALFGAYAYLRYATPIYSTNATMVISSDQAPGARNDKFEEIFGE
                     GKKVNIQSEIEVLKSKALMARVVQKQDLQFSYAVKGKIKTINIYKYGPFLIEADQLRD
                     SNSSFILKI"
     assembly_gap    4468..4536
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4639..5412)
                     /locus_tag="PK977_13075"
     CDS             complement(4639..5412)
                     /locus_tag="PK977_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRF19098.1"
                     /translation="MRFSRLFLLIVVPLYLISCKPVQQLPYYLEKVNDSTGKGEVKVP
                     ELRIQKNDLLSIQISSLSTKPELSDAIYNQVSAAGSTSAGYLVDAQGNIEHHRLGVIH
                     AEGLTKQELAAEIKKRLTVPVELLKDPTVIIRFMNFKVTVLGQVGQQGPVTVPGERLT
                     VLEAIGLAGGITDYGKKDKVKVVREINGQRETGIIDLSSKDIFDSPYYNLVQNDLLIV
                     GETNEKMKDAQQAKVMQRISFAFTIVTVAATLTNIFIRN"
     gene            complement(5461..5955)
                     /locus_tag="PK977_13080"
     CDS             complement(5461..5955)
                     /locus_tag="PK977_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRF19099.1"
                     /translation="MKKWLAVYTRPRWEKKVNQLLTEKGIESYCPLNKIRRKWSDRYK
                     IIEEPLFKSYVFVKVSEDERKDVRLTNGAINFVYWNGKPAVIKEKEIAAIRRFLDEYD
                     HVEARQVEIKVSQRVRITNGTLMDQQGTVLDIRKKTVKVAIDSLGYILIAYIDKTKLT
                     SAQA"
BASE COUNT         1610 a         1355 c         1117 g         1832 t
ORIGIN      
        1 cattcattga aaccacccac attataactg ctatgatttt ttccttcatg aaaaacaagg
       61 tcaatcgccg ccgcatggtc aataacgtac aaccagtctc ttgtatacaa accatccccg
      121 tataccggta acggcttttt atggatgata ttattgataa aaaggggaat cagtttttca
      181 ggaaaatgat tgggaccata attattggaa caattggtga taacataggg taaaccgaag
      241 gtatctccgt aggcccgaac aaaatgatcg ctgctggctt tagaggcgct gtaaggtgag
      301 tgcgggtcat acttagtttc ttctgtaaag aatcccgtat cacctaatgc accaaacacc
      361 tcatcggtgg aaacatgatg aaaacgtttg ccggtgtaat tcccattcca tagtttcttc
      421 gcagcgttaa gcaggtttac cgtaccgatc acattcgtat aaacaaaatc cagcggtgat
      481 aaaatggaac ggtctacatg gctttcagcc gccaaatgta ttacgccgtc tggttggtat
      541 tggttaaata ttcgttcaag tgctgcagta tctaaaatat cagctttctc aaaacggtaa
      601 ttaggagcac tctctatatc ccgcaagttt tccaggttac ctgcataagt caacgcatca
      661 aggttgacga ttgtatacga aggataattc gtcaccatcc tccgcaccac atgtgaacca
      721 ataaaccctg ccccgccagt aataattatg gtttttagtt gagaagttga aaggtttgtc
      781 atatcagttc gtattcttaa gtattgattt tcgcaaggca ttaattttaa aggaaactac
      841 ctcaatatct ttccttatta ttgatagttc ttcactgcta atccaactta attcattact
      901 gataataagc tgattcaaca gctccataag gctgctatag gccattccgt aaaaattcgc
      961 ctggtctttt ttactgtttc tgctgcttcc ctccgcaata tttgaactta cagatactgc
     1021 agctctgcgc atctggttta caatcccaaa tttttcatcg gaagggaatg cttttgttag
     1081 tgaataaatc ttaacaacaa gttgcttggc caattgccaa acatcaagct tttcaaatgg
     1141 ataagtatac attgaaaaaa attgttgatt tctcaaccca tcaacctctc aactcctcaa
     1201 ctcctcaact tgcctatacc atttaaacgt tctgctcaac ccctcctcta ccgaaacaat
     1261 cactttgtat cccaataatt tctctgcctt ggcaatgtcc gccaaactat gcttcacatc
     1321 accctttctt tcaggaccgt gtatagcttg caaatcactg cctgcttctt tctttaaccc
     1381 ctcaaacaat tcattcagcg atgtctgctg cccgcaggca atattataaa cctggttgat
     1441 ggcagcagca ttatcagtga acagtgaaag tatattcgcc tgcacggcgt ttgacacata
     1501 agtaaagtca cggctatgcg taccatcacc attaatagtt ggtggaaccc ctttcaaaat
     1561 tgcttctgca aataaaggta tcacggccgc ataaggccct tttggatttt gccttggccc
     1621 gaaaatatta aagtacctga gcccgatgaa ctccattcca tacaggtcag catacaccct
     1681 tgcatacagt tcattcacat acttggtcac tgcataagga gaaagtggtt taccaattac
     1741 atcttcctgt ttgggcaaac caggatgatc cccgtaggta gaagaactgg ctgcatagac
     1801 aatccgtttt atcccctttt cttttgcagc cgtaaaaatg ttcagtgtgc cggtgatatt
     1861 tacttcatta gtcgttagcg gatcattaat ggagcggggt acagatccca gtgctgcctg
     1921 gtgactgatc aaatcaaccc cctcacaggc gtttaagcaa tccgtataat ttcggatatc
     1981 accttcaatg aaacgaaaat taggatgacc gttaaaagta acaacattat ctaatgaccc
     2041 cgttgccagg ttatccagta ctgttacaaa agaaacccgg tcatcagcca gtaaggcttc
     2101 tgccaggttg gaaccaataa aaccggcgcc gccggtcacc aaaatatgca tcaggaaaat
     2161 ttatttcgcc ttctactttc tgaagggatt cagtttactg agtttatgca gtaatcgttc
     2221 aaaaaaatta tcaggggcag tttcttcctc ataataactg ctatcctgcc cgtaaccata
     2281 tccataaccg taacggccat aaccataata accataaccg gccttcattt ttacatcatt
     2341 gatcacaaca cttactttgg gcagcttctt gaattgataa aattcttcta ttagtgttat
     2401 ttgctttttg aaagtatggc cctgccttac aatatacagt gtggcatctg catacttact
     2461 cagcgtttgg gcatcactca ccatacccac aggtgctgta tctattatca ctacatcaaa
     2521 ctctttcctt aaccagtcaa acaattcatc tgtcttatca ccaagcaaca attctgatgg
     2581 attaggtgga atggggccgc aaggcaatac aaaaagattt tcatgtccgg gtacaagctt
     2641 aataaggtct ggtaatgcag agttgccaat gagaaaattg gtaatgcccg ggcctttcgg
     2701 catatttagt cctgaaatca atttgggttt acggatatca aactcaagaa taatagtctt
     2761 tcttcccgca agtgccatta ccccgccaac attggtacta ataaaagatt taccttctcc
     2821 gctataagaa gaggtgatca ggagaacaaa tttttcagct ttacctatta catattgcag
     2881 attcgaccgg ataatccgga attgttcagc taccatgctt cggttggttt tgccaaccag
     2941 caaaactgtt tctgaatagg aatgacctat ctctcccaat atcggtgcag atgttatttt
     3001 ttctacatca taacgggtag tcaccttatc attcagcatt tcactgacaa atacaaacag
     3061 ggcaggtatg gcaagtccta tcaggatagc cattaattga atggatctcc tgttaggttt
     3121 tataggcttg aaagaaacgt aggcattttc cactacctgg gaatttgcca cattggctgc
     3181 acgggaaata gctgtttgtt ctcttttttc ctgcaggatn nnnnnnnnnn nnnnngcttg
     3241 tttattatct acttgctttt tcagttcaag ataacccttg agcttataag gcatggcctt
     3301 taactgcccc tctgctaatc ccccgctttg acgaagagaa gcaatggttt tccgcatgga
     3361 atttttaata ttcgaaagat tttcgagcag gctgatacgc agtttctcta tttgttcact
     3421 ggcttctttt actgccgggt tttcggcggg cacattaccg tccagcaact gctgtcgctc
     3481 tatttgcatc gtgttgtaag cattcactaa ctcattcagg gtggggtcat taaggccgag
     3541 tgaagaggga acaataacag gcccgtagct gtttttcttg tcccgaaggt atatttcaat
     3601 atactcagca gtattcagtt gaagctgctg ttcattaata gctttgtcag atgaactaag
     3661 attatcaaaa taggccgatg tctgggcttc agcatcaatc aggttattat cctgcgcata
     3721 tcttaagaga ttattttgta cactgtcaag ttcccgtccc agtacttcca ttctgctgtc
     3781 aataaatgca atggtttgat cagatgtctg atttttcagt tctttggtat aatcaccata
     3841 ctcaaccatc agttgattga caacatcagc ggcaagattt gggttggtat tttgcatact
     3901 gatggaaaga ataccggtac cggctacttt tggcgccacc nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctaattttta
     4141 gaatgaatga tgaattagag tctcttaact gatcagcttc gatgagaaaa ggtccatatt
     4201 tgtaaatatt tattgtcttg attttaccct ttacggcata gctgaactgc agatcttgtt
     4261 tctgaaccac tctcgccatc agggcttttg atttcaaaac ttcaatctca ctttgaatgt
     4321 tcaccttttt accttctcca aaaatctctt cgaacttgtc atttctcgca cccggagcct
     4381 ggtcagacga aatgaccatt gtagcattgg tgctgtagat tggggtggca tatcgcaggt
     4441 aagcatatgc gccaaataat gctaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttag gttccacaac tcgctatggg
     4561 cctggttgtt atttgaagga ttggtatttg tattgacagt ttcttgcatc aggttattta
     4621 ctcgatgtat tgatcaggtt agtttctgat aaaaatgttg gtaagggtgg ctgcaacggt
     4681 aacaatagta aacgcaaaag aaatacgctg cataaccttg gcctgctgcg catccttcat
     4741 cttctcattg gtttctccca cgatgagcag gtcattttgc accaggttat aataaggaga
     4801 atcaaaaata tctttggaag aaagatcaat gattcctgtt tctctttgtc cgtttatttc
     4861 ccgcaccact ttcactttgt ctttcttgcc ataatcggtg atacccccgg caagacctat
     4921 cgcttcaagc accgtcagtc gttcacccgg cactgttacc ggcccttgct ggcctacctg
     4981 tcccagcacc gtcactttga aattcatgaa ccggataatt actgtcggat ccttcagcag
     5041 ctctaccggc actgttaagc gttttttgat ctcagcagcc agttcctgct tggtaagccc
     5101 ctcagcatgt atcacaccca aacggtgatg ttcaatatta ccttgtgcat ccaccaggta
     5161 cccggcactg gttgatccag ctgctgaaac ctggttatat atagcatcag aaagttcggg
     5221 tttggtactt aaactggaaa tctggataga gagcaaatcg ttcttttgaa tccgtaactc
     5281 cggcactttc acttctccct tacccgtact gtcattcact ttttcaaggt aatagggcaa
     5341 ttgctgtaca ggtttacacg aaatcaggta caaaggaaca acgatcaata aaaacaggcg
     5401 ggaaaatcgc atcggttact ccgttttttt aaagcggggt tggataatag gttttgatga
     5461 tcaggcttgc gccgaagtta atttagtttt atcaatatag gcgatcaaaa tatatcccag
     5521 gctgtcaatc gccaccttta ctgttttctt tcttatatcc agcacagtac cctgttggtc
     5581 catcaatgta ccattggtta tgcgtacccg ctggcttact tttatttcta cctgtcttgc
     5641 ttctacatgg tcatactcat ccaaaaaacg gcgaattgca gctatctctt tctctttgat
     5701 aacagcaggc ttgccattcc agtacacaaa attgatagct ccattggtga gtcgtacatc
     5761 cttgcgttca tcctcactca cttttacaaa tacatacgac ttgaataaag gctcctcaat
     5821 gatcttatac cggtcactcc acttacggcg tatcttattt aacgggcaat agctctcgat
     5881 tcctttctcc gtcaggagtt ggttcacttt cttctcccag cggggtcggg tataaaccgc
     5941 cagccatttt ttcacagtgc cattattttt cattgcacag cttcgccatc tcagtcacat
     6001 cattattttt ttacatgact ggtttacagg ctattagtaa acacttagcg ccaaaaaggc
     6061 ctgaatagtg tttaatgcct gcaaatatag gcgaacctgt gactgagaaa agaataaaat
     6121 gcacggattt tgagtgattt acccccactg gttgataact ttcgggtaat aatcctatgc
     6181 tggcatagt
//
LOCUS       DAORLS010000182         6154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6913_length_6154_cov_2.058534, whole genome shotgun sequence.
ACCESSION   DAORLS010000182 DAORLS010000000
VERSION     DAORLS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6913_length_6154_cov_2.058534"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK977_13085"
     CDS             complement(<1..443)
                     /locus_tag="PK977_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19100.1"
                     /translation="MKINRHNYEEFFLLYVDNELSSEDRHMVEDFVQLHPDLKEELEL
                     LSQFKFTPDDDIVFDNKEELLKVNGETPVSLNNYEESLLSYIDNELTEQQRIAVEQFV
                     NTNPAAKKELELLQQTKLQPETILFANKESLYRKEEKVRSLPVRW"
     gene            complement(444..929)
                     /locus_tag="PK977_13090"
     CDS             complement(444..929)
                     /locus_tag="PK977_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF19101.1"
                     /translation="MTEKEYNDCVNQYADNVYRFIVKNLRHEEDARDVVQTAFEKMWR
                     NRDGVDAAKSKSYLFTVAYHQMIDHIRKVKRIQLREEFSAETRVEDRPVSNLKKVLDE
                     ALNRLSETQKSLVLLKDYEGYSYEEIGQIMNLNPSQVKVYLHRARVQLKEYLVKMENV
                     I"
     gene            complement(1053..1286)
                     /locus_tag="PK977_13095"
     CDS             complement(1053..1286)
                     /locus_tag="PK977_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19102.1"
                     /translation="MNTKNRILTGIAILMAAAGMLVLTSSAPKSSSRNEVTEPAPSCS
                     KVKGNECAGTNSNGEFIHESLSRQFLFIAPVIF"
     gene            1487..2674
                     /gene="kbl"
                     /locus_tag="PK977_13100"
     CDS             1487..2674
                     /gene="kbl"
                     /locus_tag="PK977_13100"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRF19103.1"
                     /translation="MNEKLVQRIAKELDEIKSSGLYKTERIIESPQGAEIVVNGKTVL
                     NFCANNYLGLSSHPKVIEAAHKAIDHRGYGMSSVRFICGTQDIHKELEEKISKFLGTE
                     DTILYAAAFDANGGVFEPLFNEQDAIISDALNHASIIDGVRLCKAQRYRYEHNDMNDL
                     EAKLKEAAGARSRIIVTDGSFSMDGTIARLDEIVKLAEQYDACIMIDECHSSGFLGKT
                     GRGTHEYRGVMGKIDIITGTLGKALGGASGGFTSGRKEIIDMLRQKSRPYLFSNTLAP
                     SIVGASIAVLDMLTETTQLRDKLEYNTKYFRSKMTEAGFDIKPGDHPIVPIMLYDAVL
                     AQTMAAKLLEEGIYVIGFFFPVVAKGQARIRVQLSAAHEQQHLDKAIAAFTKIGKELG
                     VLK"
     gene            2918..3997
                     /locus_tag="PK977_13105"
     CDS             2918..3997
                     /locus_tag="PK977_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF19104.1"
                     /translation="MLLNKIFLIFLLLVSLYGLKAQERIGDKSGFTSAAFSPDGEKVV
                     TTSDDNTAKIWNAVSGELLASLSGHSDQVNSAIFSPDGNKIVTASQDYTAKLWDAASG
                     QLLADLRLHRDEVESAVFSPDGKKIVTSAGSQAKVWDAASGQLLTDLDGHSSWVKSAV
                     FSPDGKKIITTSNDLSAKLWDTEIGKLLADLRGHDDWVTSAFFSHDGQKIATASNDNT
                     AKIWDVASGKLLADLEGHSDIVNSAVFSPDGKKIITASRDNTAKIWDVASGKLLTDLE
                     GHSDVVNSAAFSPDGKKILTAGHYTVIIWDTSSDKPLIYLKGHTWAVNSATFSPDGKK
                     IVTASVDETAKIWDAKTGKLLVELK"
     gene            complement(4305..4904)
                     /locus_tag="PK977_13110"
     CDS             complement(4305..4904)
                     /locus_tag="PK977_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19105.1"
                     /translation="MKRISSILLAAIVLLAACNSDKKPGEATITSDDGKEKVTIDVNQ
                     MQQAADAMEKKKEELSKLTPLTNDQLKALLPETLMGAKRTEYNVTSAMGTSVGTADYQ
                     VNDSVNVSLTIYDCAGSGGAGIYSMQYLAMMNMEQESEEEYTKTVEFKGGKAFEHCEK
                     ATNDCTFTFFAGERFLVTLQGDHTGADALKQAAVSLNLK"
     gene            complement(5018..>5264)
                     /locus_tag="PK977_13115"
     CDS             complement(5018..>5264)
                     /locus_tag="PK977_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19106.1"
                     /translation="LHIKATFLLVVLYTLAISFGSSARAEHKRNCSRQNKAQKKSFHA
                     LILNWVNKLIKENQRNWAGRVSIIVDKTKIQSAYCIS"
     assembly_gap    5265..5279
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5461..>5664
                     /locus_tag="PK977_13120"
     CDS             5461..>5664
                     /locus_tag="PK977_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19107.1"
                     /translation="MKSLIVALLLVSACSSVSAQSSFPESWMGNWKGELQWFRTGTTE
                     PQKVNMELRIQKGDSAGNYTWHLV"
     assembly_gap    5665..5824
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1693 a         1196 c         1330 g         1760 t
ORIGIN      
        1 caccagcgaa caggcagtga acggactttt tcttctttgc ggtataatga ttctttgttg
       61 gcaaatagaa ttgtctcagg ctggagctta gtttgctgca acagttccag ctctttttta
      121 gcggcagggt tagtattaac aaactgctca acagctattc tttgctgttc tgtcagctca
      181 ttatcgatat aggaaaggag tgattcttcg taattattga gagaaacagg tgtttcgcca
      241 tttaccttca gcaattcttc tttattatcg aaaacaatat catcatcagg tgtgaacttg
      301 aactgggata ataattccag ctcttctttc agatcgggat gtaactgtac aaaatcttcc
      361 accatgtggc ggtcttcgct gctcagttcg ttatcaacgt acagcaggaa aaactcttcg
      421 taattatggc ggttgatctt catttatatt acattttcca ttttcaccaa atactctttt
      481 agttgcactc ttgcccggtg caggtaaact tttacctggc tggggttcag gttcatgatc
      541 tgtcctattt cttcataact atacccttca taatctttca gcagtaccaa tgatttttgt
      601 gtttcgctca gccggttcag cgcctcgtcc agtacttttt tcaggttgct taccggcctg
      661 tcttccaccc tggtttccgc actgaattct tcccgcagtt gtatccgctt cacttttcgt
      721 atgtgatcga tcatctggtg gtaggccacg gtaaacaggt atgacttgct tttagcggca
      781 tccactccat cccggttgcg ccacattttt tcaaaggctg tttgtaccac gtcccgggca
      841 tcctcttcgt ggcggagatt tttaacgatg aagcggtaca cattatcagc gtactggttc
      901 acacaatcat tgtattcctt ttcagtcata aacagccggg ggtttggcag gttaaaaata
      961 ggttttatga cctgcacagg aatgtaacgt cctatgcagt aaaatagtta caggcacggg
     1021 aaatattttc ttcaggcaca ggcccttgaa aatcaaaaga taacgggagc tataaacaga
     1081 aactggcggg aaaggctttc gtggatgaac tcgccgttac tgttcgttcc ggcacattca
     1141 tttcctttta ccttagagca ggaaggggca ggttctgtta cctcgttcct ggagctgctt
     1201 ttgggggctg aagaagttaa taccagcatt cctgctgcag ccatcagtat ggctatgcct
     1261 gtcagtatgc ggtttttggt gttcatgtca gctggtttgg gagtgtgacg aaactatttt
     1321 caaaaatgtt accgcaaact taaaatttct ttttacccgg tcatatcgca tattcatgtg
     1381 aaataaagga caaaaagctg ttgccatttg cgaacagatt tcaaatgctg ccggggcaac
     1441 atccatacct ggtgcataca ctatatttgc ctataaacca tgctatatga atgaaaaact
     1501 ggtacaacgc attgccaaag aactagatga gataaaatcc tcgggtcttt ataaaacaga
     1561 acgcattatt gaaagtccgc agggggcaga aattgtagtg aacggaaaaa cggtgctgaa
     1621 tttttgtgcc aacaattacc tcggcctttc cagtcacccg aaagtgattg aggcagccca
     1681 caaagcgatt gaccaccgtg gttatggcat gagcagtgtg cggttcatct gcggtacgca
     1741 ggatattcat aaagaactgg aagaaaagat ctcgaaattc ctcggtacgg aggatactat
     1801 attatatgcc gccgcttttg atgccaatgg cggggtgttt gaacccttgt tcaacgagca
     1861 ggatgccatc atctctgatg cactgaacca tgcaagtatt attgatggtg ttcggttgtg
     1921 caaagcccag cgctaccgtt atgagcacaa cgatatgaac gacctggagg ccaaactgaa
     1981 agaagcagcc ggtgcccgca gccggattat tgtaaccgat ggttcgttta gtatggatgg
     2041 tactatagca agactcgacg aaattgtgaa gctggccgag cagtacgatg cctgcatcat
     2101 gattgatgaa tgtcactctt ccggcttttt gggaaaaaca ggaaggggta cccatgaata
     2161 ccgtggggtg atgggcaaga tcgatattat caccggtaca ttaggcaagg ctttaggcgg
     2221 agcatccggt ggtttcactt ccggaagaaa agaaatcatt gatatgcttc ggcagaagtc
     2281 aaggccttat ttattcagta atacattggc cccctccata gtcggcgctt ccattgccgt
     2341 actcgatatg ctgacagaaa ccacgcagct gcgggataaa ttagaataca acactaaata
     2401 cttccgcagc aaaatgactg aagccggctt cgatatcaag cccggtgatc atcctattgt
     2461 tcccatcatg ctctatgatg cggtgctggc gcaaaccatg gctgctaaat tattagaaga
     2521 aggcatttat gtgatcggct tcttcttccc ggtagtggcc aaagggcagg caaggataag
     2581 ggtgcagctg agtgcggcgc atgagcagca gcacctggat aaagccattg ctgcgtttac
     2641 gaagattgga aaggagttgg gggtgttgaa atagttattc aactaatttg ttagaaaaaa
     2701 gacgcttcat cccgaagcat ctttttttac tatccaatta gtaaacttat ttcaaattca
     2761 aacttccggc cgcctgcttt aatgcatcag ctcctgtatg atcgccttgc agggttacca
     2821 aaaaacgttc gccggtaaag aaatgtgtgt taaagtaaca tcaaaaagtg gtattgtcct
     2881 tttgatggtt ttgctataga tttattgaaa aaataatatg cttttaaaca agatttttct
     2941 tattttttta ctgttagttt ctttatatgg cctcaaagca caagagcgga tcggggataa
     3001 aagtggtttc acttcagccg ccttcagtcc cgatggggaa aaggtagtca ctacatcaga
     3061 tgacaacact gctaaaatat ggaatgcggt tagcggtgaa ttgctcgcta gtttgtccgg
     3121 gcatagtgat caagtcaatt cagctatttt tagtcccgat ggaaataaaa tagtaactgc
     3181 atcccaagac tacaccgcta aactatggga tgctgctagc gggcaattgc tggccgattt
     3241 gagattgcat agggatgaag tagaatcagc ggttttcagc ccagatggaa aaaagattgt
     3301 tacttccgca ggcagtcaag ctaaagtatg ggatgctgct agcgggcaat tgctgaccga
     3361 tttggacggg catagtagtt gggtaaaatc agcagttttc agcccagatg gaaaaaagat
     3421 aatcactacg tctaatgatt taagtgccaa actatgggat acagaaatcg gaaaactttt
     3481 ggctgatttg agggggcatg atgattgggt tacttcggcc ttctttagcc acgatgggca
     3541 aaagatagcc actgcatcta atgacaatac tgccaaaata tgggatgtag caagcgggaa
     3601 gttgctggct gatttggaag gtcatagtga tattgtgaat tcagccgtct tcagccccga
     3661 tgggaaaaag ataatcactg catccagaga caatactgcc aaaatatggg atgtagcaag
     3721 cgggaagttg ctgactgatt tggaaggtca tagtgatgtt gtgaattctg ccgccttcag
     3781 tcccgatggg aaaaagatac tcacggctgg gcactataca gtcataatat gggacacatc
     3841 gagtgataaa ccactaattt atttgaaagg acatacctgg gctgtaaatt cagctacctt
     3901 tagcccagat ggaaaaaaga tagttactgc atcagtggac gaaacagcca aaatatggga
     3961 tgccaaaact ggaaaattac ttgttgaatt aaagtaaagt agtgacgcaa ataaatcatc
     4021 tttgattaga agatcaactc tgtttatcca atctggctga aagaatgata aatgaaaaaa
     4081 atcagtagca aaagtgtaaa tatgatcagt ttatccaaat taggaataac caatgcagtg
     4141 gacagatgta tttttttaga ataatcctgc acagatactt gtagatcgaa agattggata
     4201 tcaaaaaaca atttatatat aaaatagcgg tgaattggag tttgtggtaa ataaaaaaga
     4261 cgcttcatca caaagcgtct tttttactat ccgattaata gcacttattt caaattcaaa
     4321 ctcactgctg cctgcttcaa tgcatcagct cctgtatgat cgccttgcag ggttaccagg
     4381 aaacgttctc cggcaaaaaa tgtgaaagtg cagtcgttgg tggctttttc gcaatgctca
     4441 aaagctttgc cacctttgaa ttcaacagtc ttggtatatt cttcttcact ttcctgttcc
     4501 atgttcatca tagcaagata ttgcatactg taaatacctg ctccgccgga accggcacag
     4561 tcatatattg tgagacttac attcacactg tcattcacct ggtagtcggc ggtacctaca
     4621 ctggtgccca tagcgctggt aacattatat tcggtacgct tggcgcccat tagtgtttcg
     4681 ggtaataagg cttttagttg atcatttgta agcggggtga gcttacttaa ttcttctttt
     4741 ttcttttcca tcgcatcagc ggcctgctgc atctggttta catcgatggt cactttttct
     4801 tttccgtcat cactggtaat ggtggcttca cccggttttt tatcgctgtt acaagctgcc
     4861 agtaatacga tggctgccag caaaatggag gaaattcgtt tcataattaa tgatttagat
     4921 ttataaggtg tgtaaaagta cagtaattag ggattttacg atatgagttt tgacatcgta
     4981 gatttccgat ggtggcaaag aaaaattccg gagaaaacta tgaaatacaa tatgcggatt
     5041 gtattttcgt tttgtctacg atgattgaaa ctctacccgc ccaattcctt tgattttcct
     5101 taattaattt gttgacccaa tttaagatca aagcatgaaa agattttttc tgagccttgt
     5161 tttggcggct acagttgcgt ttatgttcag ctcttgcgct gctaccaaaa gagattgcca
     5221 gggtgtaaag cactacaagc aaaaaggtgg cttttatatg tagtnnnnnn nnnnnnnnnc
     5281 agattattta tcccggcttt tttagccggg atttttattt ttgataaaat atcttttatg
     5341 aaatgagcca tcataaaata ttcattgctt tacctacttt aaatgaatat tttattttgg
     5401 cgaacctgcc tgccggcaag caggtaacat gagacaaatt ccaaaaacta aaatatactc
     5461 atgaaatcgc ttatagtcgc tttgttactg gtctccgctt gttcttcagt ttctgcccaa
     5521 tcctcctttc cggaaagctg gatgggcaac tggaagggag agttgcagtg gttcagaaca
     5581 ggtacaacag aaccgcaaaa agtaaacatg gaactgcgta tacaaaaagg cgacagcgcc
     5641 ggtaactata cctggcacct tgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnaataat tattggttgg aagatggcaa gctgatgatt gaattttaca ctctcgctgc
     5881 caaaccgctt atcactaccg gtttgggcac ggaagaaagt cccaaagtgg acagttaccg
     5941 ggtaggcggt tatcaaaaag caattcttag caggataaat taatgcccgt tacctttgcg
     6001 gaaattttga ttgatgataa gaagtgtcgt ttcctttctt ttagtaattg cattactgtc
     6061 agcatgttct tccaacaatg taaaacaaga caagagcctc caaaaatatt ttgatgaaaa
     6121 taaagtagaa ggctgctttg ccataatgga taat
//
LOCUS       DAORLS010000183         6141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6929_length_6141_cov_2.172691, whole genome shotgun sequence.
ACCESSION   DAORLS010000183 DAORLS010000000
VERSION     DAORLS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_6141_cov_2.172691"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK977_13125"
     CDS             complement(<1..436)
                     /locus_tag="PK977_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRF19108.1"
                     /translation="MHALIIKELMEYSKLVAVTGLPGLFELLNSKTDGAIVRSLEDKS
                     TRFVSSRIHNFSHLESIEVYTIRDNVNLVDVFNAMDKAGGSLPSDKDADAVKKYFEKV
                     YPDMDFERVYASDMKKMVKWFDILKKNNVEIKLSEQPEEEAVT"
     gene            556..963
                     /locus_tag="PK977_13130"
     CDS             556..963
                     /locus_tag="PK977_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19109.1"
                     /translation="MKKILICLLALFGTAAVVSAQPGGGMRRTPEERAAVIHQKLDSA
                     FKLEASKLAMLDTALVALYKAQDAKRQEMMAGGSFDRDAFMEEMKKFNDAQDEMLKAV
                     LSKDQYEIWKEKIQPAMRPQRQGGSGRPGGGNR"
     gene            1109..1624
                     /locus_tag="PK977_13135"
     CDS             1109..1624
                     /locus_tag="PK977_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19110.1"
                     /translation="MKNLLFTICISFFTIPLFAQKPCSQPVFRQFDFWIGEWEAFGPK
                     GAKAGDSKITVLLDSCVILEEWTSANAQQGLIYSGKSFNSYNAATKQWQQTWTDNTGN
                     TTEFLRGEGSDGKIVFYADKVTDPKGKTFMRRLTFTKISEDKVRQFGERSNDGGNTWT
                     VEYDLEYRRKK"
     gene            1621..>2184
                     /locus_tag="PK977_13140"
     CDS             1621..>2184
                     /locus_tag="PK977_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19111.1"
                     /translation="MRYLSYITIVLIAVLVACSNKKRTNYFSGQISYTYSYTSDSLNT
                     DSISAIRPSQGVFWYDTSNYQSRFIGADTFTYYYSGKLNKCLFEPGSSKEYECEDYSK
                     ETDSVVYVKDYPTDEKILGYPCRILELQKKSSWVKYYYATDLQIALATYKNHKAYNWD
                     VYGIKTNGGLILKLEHRFNKFVMHGLAN"
     assembly_gap    2185..2313
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2314..2618
                     /locus_tag="PK977_13145"
     CDS             <2314..2618
                     /locus_tag="PK977_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19112.1"
                     /translation="LLGNCFALYKMFTAKQEFFDQFPKLTEKAFNIFRLLPMINLIAL
                     AGMWWMKPWAAYLIITCSIAVIAFDIFFGIWYHLYVAIPSTFIFLFFVIKYWNGFK"
     gene            2690..3805
                     /locus_tag="PK977_13150"
     CDS             2690..3805
                     /locus_tag="PK977_13150"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF19113.1"
                     /translation="MRKLILSLIASLLISSIHAQMDIPPSGNNPRATISEEVGITSIT
                     LKYSRPDVNKREGKVYGDGNLVPYGFSTTNFITGKNTSPWRAGANEATTISFEHDVKV
                     EGKDIVAGTYAIFMAMGPDDITLIFSKQVDAWGSFYYKEEDDVLRVKVKPVLLDKSVE
                     WLKYEFIEHKEKSCVIAMQWEKVSVPFKVEVDVDNIVLARLRQQVTSQKGFNSNNMLQ
                     AAQWCLNKNINLEEALQWSIRAINGFQGQKSFITLRNLANAYDKLSRTVQADSTMDEA
                     LTIATANQYTAYGRQLITQKRVDRAMDIFRASEKRYGDMYGVNNGLMSGYSAKGDFKY
                     AIKHAEKAMAQAPGEAAKKQIEEYIKKLKDGKDINQP"
     gene            3780..4238
                     /locus_tag="PK977_13155"
     CDS             3780..4238
                     /locus_tag="PK977_13155"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF19114.1"
                     /translation="MAKTSTNPKKKLAELKTKENTGDVGAFIAAAGSDEKVKDSFALL
                     KMFSSITRQKPMMWGSSIVGFGKYIYTSPKTGRSGEWMMTGFSPRKQNLTIYFMTGFS
                     NYGELMKKLGKYKISGGSCLYINKLADIDMKVLKEMITASWKHMKEKYQN"
     gene            4250..4633
                     /locus_tag="PK977_13160"
     CDS             4250..4633
                     /locus_tag="PK977_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF19115.1"
                     /translation="MQHAISWFEIGTTDLERATKFYETIFGIQLIPMDLPNIKMRMFP
                     LDDMMTQVGGALVDSGGFHKPSSTDGPLVYLNGNPDLQKVLDKVEAAGGKIMVPKTEI
                     SPEYGFMAVIIDTEGNRIGLHSVPA"
     gene            4676..5203
                     /locus_tag="PK977_13165"
     CDS             4676..5203
                     /locus_tag="PK977_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF19116.1"
                     /translation="MIVLYILLGIIAALLLAGLVISKDIKATKEIVINKPVNEVFNYI
                     KYLKNQQHYSKWATLDPNMKNEFRGTDGQPGFVNSWSGNKKVGQGEQEITAIEEGRAL
                     HTDLRFIKPFKSFARAKMTTEAIDAGSTRVKWGFESKMNYPMNVMKLFMNMSEMVGKD
                     FSTGLSNLKNVMEKQ"
     gene            5200..5700
                     /locus_tag="PK977_13170"
     CDS             5200..5700
                     /locus_tag="PK977_13170"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRF19117.1"
                     /translation="MMKYGFVLFIAAVLIVACNNNNEKRDDFQSRQNVTIFSWLNGQW
                     AMTEKEGTITEEWKPVNDSLMEGKSDLVTGDSVIPFETIRIFREDTEFYYEAKAAGQN
                     KEQPVVFKVTSFSDTGFVAENPQHDFPKRISYTLINKDSIHAFVDGGAAMPNKKSDYY
                     YSRVKN"
     gene            5704..>6141
                     /locus_tag="PK977_13175"
     CDS             5704..>6141
                     /locus_tag="PK977_13175"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF19118.1"
                     /translation="MSEANWSSFIIRIPVNATTEKLYWCWATKEGMEYWFLRLSEYKR
                     PDGSLLNNNEAVQPGDMYKWLWYGWPDDTVENGKILDCNGRDMLKFSFGHAGNCTVTI
                     KKENGQSIVELIQDNIPDDEHGKYYWHVGCKTGWTFYFTNLKSL"
BASE COUNT         1992 a         1051 c         1324 g         1645 t
ORIGIN      
        1 cagttacggc ttcttcctca ggttgttcag aaagtttaat ttccacattg ttctttttca
       61 gaatatcaaa ccatttcacc atcttcttca tatcactggc atataccctt tcaaaatcca
      121 tatcagggta tactttttca aaatattttt ttactgcatc agcatcttta tcagatggca
      181 ggcttcctcc tgccttgtcc atagcattaa aaacatctac caggttcaca ttatcacgga
      241 tagtgtatac ttcaatactc tccaggtgtg agaaattgtg aatacggctg gatacaaacc
      301 gggtagactt gtcttcaaga gagcggacaa tggctccatc ggttttgctg ttgagcagtt
      361 caaacaaacc cggcaacccg gttacggcaa ctagtttact gtattccata agttctttga
      421 ttattagggc atgcaagata cttgagttcg ggcaaaaagg gtgttttgaa aaaataaatc
      481 tgaccttccc gaacggtttt tatactttca tcgtcatata aaaagttcca tataaacact
      541 tgtaaataaa tatatatgaa gaaaatactt atttgcctgc tggctctctt tggtactgca
      601 gctgttgttt cggctcagcc cggtggtggt atgcgccgca ccccggaaga aagagctgct
      661 gttattcacc aaaaattaga tagcgccttt aaattggagg cttccaaact ggccatgctg
      721 gatacagcac tggtagcttt atataaagca caggatgcca aaagacagga aatgatggcc
      781 gggggcagtt ttgaccggga tgcattcatg gaagaaatga aaaaattcaa tgatgcccag
      841 gatgaaatgc tgaaggcagt gctttccaag gaccaatatg aaatctggaa agaaaagatc
      901 cagcctgcca tgcgaccgca acgacaaggc ggcagcggcc gtccgggtgg cggaaatcga
      961 taaatctttt acgattagta agttattaag ccgttctgaa caaacagaac ggcttaattt
     1021 tttttaactt gttgtttggc caaataaaat gacaggcgga atgagacagg cggctgaaca
     1081 tttgtctgta aacgttaaac tggttaaaat gaaaaattta ttattcacca tttgtatctc
     1141 attctttact ataccacttt ttgcacaaaa gccatgctct cagccagtgt tccgccagtt
     1201 tgatttctgg attggcgaat gggaggcatt tggtccaaaa ggcgccaagg caggagacag
     1261 caaaataacc gtgctgttgg acagttgtgt tattctggaa gaatggacca gtgccaacgc
     1321 ccaacagggc cttatatata gtggtaaaag cttcaattcc tacaatgctg ctaccaaaca
     1381 atggcagcaa acctggaccg ataataccgg caacaccaca gaatttttaa gaggtgaagg
     1441 cagcgatgga aaaattgttt tttatgctga taaagtaact gatccgaagg gaaaaacatt
     1501 tatgcgccgc cttaccttta ccaaaatcag tgaagacaaa gtaagacagt ttggcgaaag
     1561 aagtaatgac ggaggaaaca catggactgt agaatatgac ctggaataca ggagaaaaaa
     1621 atgagatatt tatcatacat aactattgtt ttaatagctg tattggtggc ttgcagtaat
     1681 aagaaacgca caaattattt ttccggccaa atcagttata catattctta caccagtgat
     1741 tcgcttaata ccgactcaat atctgcaata aggccttccc agggtgtatt ctggtatgat
     1801 accagtaatt atcaaagcag gtttattgga gcagacacat ttacttacta ttactcaggg
     1861 aaattgaata aatgcctctt tgaacctgga tcttcaaaag aatatgaatg tgaagattac
     1921 agtaaggaaa ccgactccgt agtttatgta aaagactatc caactgatga aaaaatattg
     1981 ggttatccct gccgaatcct agagttgcaa aagaaaagct catgggtaaa atactattat
     2041 gccactgacc ttcaaattgc gctggctacc tataaaaatc ataaagctta caattgggat
     2101 gtttatggta taaaaaccaa cggtgggctg atacttaaac tggaacatcg gttcaataag
     2161 tttgtaatgc atgggctggc aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgctgct gggcaattgt tttgccctgt
     2341 ataaaatgtt caccgcaaag caggagtttt ttgatcagtt cccgaaactg actgaaaaag
     2401 ctttcaatat atttaggcta ttacccatga taaacttaat agccctggca ggtatgtggt
     2461 ggatgaagcc ctgggcagca tacctgatta taacatgcag cattgctgtt attgcctttg
     2521 atattttttt cggtatttgg tatcacctgt atgtggccat tccctccacc tttatctttt
     2581 tgttttttgt tattaagtac tggaacgggt ttaaatgagc atttgccgtg ataagcggca
     2641 actgcaacac gatatttttt ggtaatttaa atgtatcaaa actcgattta tgagaaaatt
     2701 aattctttct cttatagcct cactattgat aagcagtatt catgcacaaa tggacatacc
     2761 gccaagtggc aataatccaa gggcaaccat ttctgaagag gtgggcatta cttccattac
     2821 tttaaaatat agccgcccgg atgtaaataa aagagaagga aaagtttatg gggatggcaa
     2881 cctggtacct tatgggttta gcaccaccaa ttttataaca ggaaaaaaca cttctccgtg
     2941 gcgtgccggc gccaatgaag ctactactat cagttttgaa catgatgtaa aagtggaagg
     3001 aaaagatatt gtggcaggta cttatgctat attcatggct atgggccctg atgatattac
     3061 acttatattt tctaaacagg ttgatgcctg gggcagtttt tattataagg aagaagacga
     3121 tgtattaagg gtaaaggtaa aacctgtact actggacaaa agtgtagagt ggctgaaata
     3181 tgaatttata gaacataaag aaaaaagttg tgtaattgcc atgcagtggg aaaaagtttc
     3241 tgttcctttt aaagtggaag tagatgtaga caatattgtg ctggcaagat tacggcaaca
     3301 ggtaaccagc caaaagggat ttaatagtaa taatatgcta caggcagcgc aatggtgttt
     3361 aaataaaaac atcaatcttg aggaagcctt gcagtggagt ataagggcta tcaacggctt
     3421 ccagggacaa aaaagcttta taacgcttcg caacctggca aatgcttacg ataagttaag
     3481 cagaactgtg caggctgatt ccactatgga tgaggcatta acgattgcaa cagccaatca
     3541 atacactgcc tatggccgcc agttaattac gcaaaaaaga gtggaccggg cgatggatat
     3601 ttttagagcc agtgaaaaaa gatatggaga tatgtatggc gtaaataacg gattaatgag
     3661 tggttactca gccaaaggtg attttaagta tgccattaag catgcagaaa aggcaatggc
     3721 gcaggcacca ggtgaggcgg caaaaaagca aattgaagag tatatcaaaa aactgaaaga
     3781 tggcaaagac atcaaccaac cctaaaaaga aattagctga gcttaaaaca aaagaaaaca
     3841 caggtgatgt gggggcattt atagcggcag caggcagcga tgaaaaagta aaggatagct
     3901 ttgcgttatt aaaaatgttt tcatcaataa ccaggcaaaa gccgatgatg tggggcagca
     3961 gtatagttgg ttttggtaaa tacatttata cttctcctaa aacaggccgt agtggtgaat
     4021 ggatgatgac aggtttttca ccacgtaaac aaaatctgac tatttatttt atgacaggat
     4081 tctcaaacta tggtgaattg atgaaaaaat taggaaaata taaaatcagt ggtggaagct
     4141 gtctatatat aaataaactg gctgatatag atatgaaagt gctgaaggaa atgataactg
     4201 cttcgtggaa acacatgaag gaaaaatatc aaaactaaaa tcaatcaata tgcaacatgc
     4261 tatcagctgg ttcgaaatag gaaccacaga cctcgaaaga gccactaagt tttacgaaac
     4321 tatttttggt attcagttaa tacccatgga cctccccaat ataaaaatgc ggatgtttcc
     4381 gctggatgat atgatgacac aggtaggcgg tgcattagtt gacagcggcg gatttcataa
     4441 gccttcgtcg acagacgggc cattggtata tctgaatggt aatcctgatc tacaaaaagt
     4501 gttagataaa gtagaagccg ccggaggaaa aattatggtg ccaaaaacag aaatcagtcc
     4561 tgaatatggt tttatggccg ttataataga tacggaaggc aacaggatcg gattacatag
     4621 tgtgcctgca tagaacaata ttgtataaca tttttaaaat accaaaacta acacaatgat
     4681 agtattatac atcctacttg gcatcattgc ggcattactg ctcgccgggc tggttatcag
     4741 taaggacata aaagctacca aggaaatagt gattaacaag cctgtcaatg aagtttttaa
     4801 ttatataaaa tatttaaaga atcagcagca ctacagcaag tgggcaacgc ttgaccctaa
     4861 tatgaaaaat gaatttcggg gaacagacgg gcagcccggt tttgtaaatt cctggtcagg
     4921 taacaagaaa gtcggacaag gagaacagga aatcaccgcc attgaagagg gaagggcttt
     4981 acataccgat ttgcgattca ttaaaccatt taagtcattt gccagggcaa aaatgacaac
     5041 tgaggcaatc gatgccggca gtaccagggt caaatggggt tttgagagta aaatgaatta
     5101 cccgatgaac gttatgaagc tctttatgaa tatgagtgaa atggtgggta aggattttag
     5161 tactggcctt tccaatttaa aaaacgtaat ggaaaagcaa tgatgaagta tggcttcgtt
     5221 ttatttatag ctgcagtact tattgtagcc tgtaataaca ataatgaaaa acgggatgat
     5281 tttcagtctc ggcagaatgt taccatcttt agctggttaa atggccaatg ggctatgacg
     5341 gaaaaagaag ggacaattac tgaagaatgg aagcctgtaa atgacagcct gatggaaggc
     5401 aaatctgatc ttgtgacggg cgactctgtc atcccttttg aaacaatcag gatattcagg
     5461 gaggatactg agttttatta tgaagccaaa gcagcaggcc agaataaaga acagccggtt
     5521 gtttttaaag tcacttcatt ttctgataca ggatttgttg cagaaaaccc acagcatgat
     5581 tttcccaagc gaattagtta tacattgata aataaggatt ctattcatgc atttgttgat
     5641 gggggagcgg cgatgccaaa caaaaaatct gactattatt attcaagagt aaaaaactaa
     5701 actatgtcag aagctaattg gtccagtttt ataatacgaa tacctgtaaa tgcaacaacc
     5761 gaaaagctat attggtgctg ggctacaaaa gagggaatgg aatattggtt tctccggcta
     5821 agcgaatata aaagacccga tggctcgcta ctgaataata atgaagcggt tcagccaggc
     5881 gatatgtaca aatggctgtg gtatggttgg cctgatgata cggtggagaa tggcaaaatc
     5941 ctggattgta acggaagaga tatgctgaaa ttcagttttg gccatgcagg taactgtaca
     6001 gttactatta aaaaggaaaa tgggcaaagt attgtggagc ttattcaaga caatatcccg
     6061 gatgatgaac atggcaaata ttactggcat gtgggatgta aaaccggctg gacgttttat
     6121 tttacgaatc tgaaatcttt g
//
LOCUS       DAORLS010000184         6141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6930_length_6141_cov_1.937562, whole genome shotgun sequence.
ACCESSION   DAORLS010000184 DAORLS010000000
VERSION     DAORLS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_6141_cov_1.937562"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>245
                     /locus_tag="PK977_13180"
     CDS             <1..>245
                     /locus_tag="PK977_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRF19119.1"
                     /translation="KWFFDQGCSLVILACNTASAKALRTIQQNDLPKIAPGKRVLGVI
                     RPTAEVIGSYSETKEVGVLATNGTVLSESYPMEIGKF"
     assembly_gap    246..389
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <390..>644
                     /locus_tag="PK977_13185"
     CDS             <390..>644
                     /locus_tag="PK977_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRF19120.1"
                     /translation="GCTHYPLLKDKIEEYLPVGVKLISQGEIVANSLANYLVRHPEIE
                     TRCARNGKRLFYTTDSTGDFDSHGFLFYGAPVQSTHVDLG"
     assembly_gap    645..666
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            831..986
                     /gene="rpmH"
                     /locus_tag="PK977_13190"
     CDS             831..986
                     /gene="rpmH"
                     /locus_tag="PK977_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRF19121.1"
                     /translation="MKRTFQPHRKRRKTVHGFRKRMQTANGRKVLASRRAKGRKKLTV
                     SDERKLK"
     gene            1056..1454
                     /gene="rnpA"
                     /locus_tag="PK977_13195"
     CDS             1056..1454
                     /gene="rnpA"
                     /locus_tag="PK977_13195"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRF19122.1"
                     /translation="MAKQFTFGKQERLKSRKAIELLFQSGKRFSVGSLRVFYLEQPAP
                     GLVAGVGVSSKNFKKATDRNKVKRLTREVYRLQKLHLQEKLAAAGIGLHVFFIYTSKD
                     LPVFTDLFETMTVMLNRLVKYFDENTAANT"
     gene            1429..1659
                     /gene="yidD"
                     /locus_tag="PK977_13200"
     CDS             1429..1659
                     /gene="yidD"
                     /locus_tag="PK977_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRF19123.1"
                     /translation="MKTLLQILSLPFIALIKIYQWVISPWLGPKCRFTPTCSHYAAEA
                     LKKHGVFKGMWLAIKRISRCHPWGGSGYDPVP"
     gene            1736..2353
                     /locus_tag="PK977_13205"
     CDS             1736..2353
                     /locus_tag="PK977_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF19124.1"
                     /translation="MISVSIIEDDKHYREGLETLIGNSPDFVVLHSYPSAEIALPHIV
                     THPPRIAVVDIKLPGRNGIDLIGAIKKASPDIQCMVCSFYDDDEYVFNALKNGACGYI
                     LKDSMPNDILISLKELHEGGAPMSRYIARKVINTFQAEPAQPRLTELTDRENEILQLI
                     ATGMIVKEISTRLFLSAHTVTKHLKNIYTKLHVNNRIEAVNRLKQ"
     gene            complement(2355..3023)
                     /locus_tag="PK977_13210"
     CDS             complement(2355..3023)
                     /locus_tag="PK977_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRF19125.1"
                     /translation="MLQPATIKFLKDLKKNNNKPWFDKNRKIYEAAKADFASFIQQVI
                     DLHSKKDNTIKNLLAKDCMFRINRDIRFSKNKSPYKTNFGASINKAGRKAMNAAGYYF
                     HLEPGGSFMGGGLYMPMPGELKNVRQEIDYNFAAFQKIIGAKKFKSIYGDLHKSKEFL
                     LSRLPRGYEPGNPADEYIRLKSYIAMTTISDKDLGSKDLLKKTVAAFDALHPLIEFIN
                     KSFE"
     gene            complement(3102..3662)
                     /locus_tag="PK977_13215"
     CDS             complement(3102..3662)
                     /locus_tag="PK977_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HRF19126.1"
                     /translation="MIKKLAGEIWKPLNFSGWRHLRNRYAVSSFGRIASYKQNVLEDG
                     KLLSGSLTTGYRTLNLHRPGNKGTLYIHRELAKLFLPKSSPQHKYVIHRNHNKLDNHI
                     KNLRWATLEQMVSHQQGSPAKIAYKEKQANKTEGLKLTAADVKKIKKILNSKNRSITI
                     RQLAKNYGVSEMTMYRIKSGENWGRI"
     gene            complement(3776..5878)
                     /gene="ligA"
                     /locus_tag="PK977_13220"
     CDS             complement(3776..5878)
                     /gene="ligA"
                     /locus_tag="PK977_13220"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRF19127.1"
                     /translation="MYSKETTHQLQKLTNELLTKLKAGQIKSKNVQELGDVLRFHEYR
                     YYILNDPLIADYEYDQLFKCLEKIEQETPTLKMPDSPTQRVAKGLTKDFPTVQHLVPM
                     LSLDNSYNSDDLLDFDRKARELTGLKEIEYCVEPKFDGSSISLIYENDLLVRGATRGD
                     GVEGDDITINIKQIRSIPLSAKFSEYGITQVEIRGEVLINKKNFAKYNEQLAEQGLPP
                     LANPRNAAAGTLRIKDTVEVGKRNLEAFVYHVSFYTTEKGIKTPVELSTHSDSLALLW
                     NLGFRSPEKEKKVFKGIEKVIKYCNEFEEGRDDLPYEIDGMVIKVNDTALQDKMGMTS
                     HHPRWAIAYKFKARQATTELLGVEFQVGRTGAVTPVAKLKPVAIGGVTVSSISVHNED
                     YIKEKDLRIGDSVLIERAGDVIPQIVKPLADTRTGHEKKIHFPKTCPVCNSKLFKEEE
                     EAVWRCINIDCPAQVVERIIHFVSKDAMDIRGFGEANVRKFYELGLLKDIPGIYTLDF
                     AALSGMEGFGQKSIENLHAAIENSKKQPLHRLIYALGIRFAGETTAKTLAQAVNHLLD
                     FKKFSIEDLQNLEDVGPKVAGSIHHFFSNRSNIEMLEELEKLGLQLKNTKKEMTTTGN
                     LSGQTFLFTGTLPTLKRSEAEALAEENGGQILSGVSAKLNYLVVGEDAGSKLEKAKKI
                     NSVKIISEEEFLQLVGKK"
BASE COUNT         1669 a         1249 c         1177 g         1880 t
ORIGIN      
        1 taaatggttt tttgatcagg gctgttcgct ggttattctt gcctgcaata cagcttccgc
       61 aaaagcatta cgcaccatac aacaaaatga tctgccaaaa atagcgccag gtaaaagggt
      121 gttgggagta atacgaccca cagcggaggt gattggttcg tattctgaaa caaaggaagt
      181 aggagtactg gcaaccaacg gtaccgtatt gtccgagtct taccccatgg agattggcaa
      241 gttttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aggatgcaca cactaccctc tactaaaaga
      421 taagatcgag gaatatttac cggtaggtgt caaacttatt tcgcagggag aaattgtagc
      481 aaacagcctt gccaattacc ttgtccgcca cccggaaata gaaacccgct gtgccagaaa
      541 tggtaaacgc cttttttata caacagattc taccggtgat tttgacagtc atggatttct
      601 attctacgga gcaccagtac aatctaccca tgtggatctc ggtannnnnn nnnnnnnnnn
      661 nnnnnnagac ttaatgctta aaaaagcttc atttttgatc ccgggttctg atctcattcc
      721 cttacctttg ccgtcctttc acaaagcagc ggatgtggtg atctgcgcta aactgaaacc
      781 cggaccgttc tcccggtcta catttcaaat agtgaaaaag agataaaaca atgaaacgta
      841 catttcaacc tcatcgtaaa cgccgcaaaa ccgtacatgg ttttcgtaaa cgtatgcaaa
      901 ctgccaatgg ccgtaaagta ctggcaagcc gccgtgcaaa aggccgtaag aagctgacag
      961 tttctgatga aagaaaatta aaatagcaat atagttaccc taaacggggt acaagcttat
     1021 tatatttata gctccggaaa cggagctttt ttattttggc aaaacaattc acattcggaa
     1081 agcaggaacg gctgaaaagc cgcaaagcga ttgaactgtt gtttcaatca ggtaaacgtt
     1141 tttctgtagg tagccttcgt gttttttacc tggaacagcc ggcgccgggc cttgtagccg
     1201 gggttggggt atcatccaaa aattttaaaa aggcaacgga ccgaaacaag gtgaaaagac
     1261 ttacacggga agtgtaccgc ttgcagaaat tacacttgca ggagaaactt gctgctgcag
     1321 ggataggctt gcatgttttt ttcatttaca catctaaaga cctgcccgta tttactgatt
     1381 tattcgaaac aatgacggta atgctgaacc gcctggtaaa atacttcgat gaaaacactg
     1441 ctgcaaatac ttagtctgcc tttcattgca ctgataaaaa tttaccagtg ggtaatttct
     1501 ccctggttgg gtcctaagtg ccggtttacc cccacctgtt cacattatgc agctgaggca
     1561 ttgaagaagc atggagtttt taaaggtatg tggctggcta ttaaaagaat ttccaggtgt
     1621 catccctggg gtggcagcgg gtatgatcct gtaccctaac ggtaacatac agaggtattt
     1681 tgctctgaca aacttcagcg aatattctgt atcttgtttg taagaaataa agaccttgat
     1741 atcagtatca attattgaag atgataagca ttaccgggaa ggattagaaa cgctaatcgg
     1801 gaattccccg gattttgttg tactccacag ttatccgtcc gcagaaattg ccttaccaca
     1861 tattgtcaca catcctcccc gtattgcggt tgtagatata aagctgcccg gaagaaacgg
     1921 tatagacctg ataggagcca ttaaaaaagc atcacccgat atacaatgta tggtttgttc
     1981 attttacgac gatgatgaat atgtttttaa tgcgctgaag aacggggcat gcgggtatat
     2041 tttgaaagat tcaatgccca atgacattct tatttccctg aaggaacttc acgaaggggg
     2101 tgcacccatg agccgctata ttgcccgtaa ggtgatcaat acttttcagg ctgaacccgc
     2161 ccaacccagg ctgactgaac tgacagacag agagaatgaa attttgcaac taattgctac
     2221 aggcatgatc gtaaaagaaa tatcgacccg tcttttcctg agtgcccata ccgtaaccaa
     2281 gcatctcaag aatatttata caaaactaca tgtgaacaat aggatagaag ctgtaaacag
     2341 gctgaaacag taagctattc aaaactttta ttgataaact caataagggg atgtaaagca
     2401 tcgaaagcag caacagtttt cttcaagagg tcttttgagc ccaggtcttt atctgaaatc
     2461 gttgtcattg cgatatagct tttcaacctg atgtactcat cagcaggatt accaggctca
     2521 tatcccctgg gtagccggct cagtaaaaac tccttactct tatgcaggtc gccatagatt
     2581 gatttgaact ttttagcacc aattattttt tggaatgcag caaaattgta gtcaatctct
     2641 tgtcttacat tctttagctc tcccggcatt ggcatataca atcccccgcc cataaaagaa
     2701 ccgcctggtt caagatgaaa ataataaccg gcagcattca tagcttttcg gcctgcttta
     2761 ttaatactgg caccgaagtt tgtcttataa ggggatttgt tttttgaaaa gcggatgtca
     2821 cggtttatac gaaacataca atccttagca agcaagtttt taattgtatt atcctttttg
     2881 ctatgcaggt caattacctg ttgaataaaa ctggcaaaat ctgctttagc agcttcataa
     2941 atttttcggt tcttatcaaa ccagggtttg ttattattct tctttagatc ttttagaaat
     3001 ttaatggtag caggctggag catagagcag taattaaaca gaattattga gataaagata
     3061 cctaaccttc cagctaataa tgaaatttag tatctgtcct tctaaattct tccccaattt
     3121 tcgccacttt taatgcggta cattgtcatt tcgctaacac catagttttt ggcaagctga
     3181 cgaatggtaa tactccggtt tttgctgtta agtattttct tgattttttt tacatcagcc
     3241 gctgtcagtt ttagtccttc cgttttgtta gcctgttttt ctttgtaagc tatcttggcg
     3301 ggggatcctt gttggtgact gaccatttgt tccaatgtag cccagcgcag attttttata
     3361 tggttatcca gtttattatg attacgatga attacatatt tatgctgggg tgatgatttg
     3421 ggtaaaaaaa gtttagccag ttccctgtga atgtataatg ttcctttatt cccggggcga
     3481 tgaagattta atgtacgata gcctgtcgtt aatgagccac ttaacaattt tccgtcttcc
     3541 aaaacattct gtttataact ggcaatacgg ccaaaactgg aaacagcata cctgttgcgg
     3601 agatgcctcc atccggaaaa attgagcggc ttccagatct caccggctaa tttttttatc
     3661 ataacaaatg tttttagcca ggcacaggat gcattgaata acaatggtac gcagaagtta
     3721 tccttgcaaa aaaggaactc ctactaagtt agaacaaata cccttgctac caattttatt
     3781 tttttccaac cagttgcagg aattcttctt cagaaatgat tttgacagag ttgatctttt
     3841 ttgctttttc cagtttgctg ccggcatcct caccaactac gaggtaattt agtttagcgc
     3901 ttacaccact cagtatttgt ccgccatttt cttctgccag ggcttccgcc tcgcttcttt
     3961 tcagggtggg tagtgtacca gtgaatagaa aagtttgacc tgacaagtta ccagtagtag
     4021 tcatttcttt cttggtattc tttaattgca agccaagttt ttccagctcc tccagcattt
     4081 ctatattact tctgttgcta aaaaaatgat ggatactacc tgcaaccttt ggtcccacat
     4141 cttccaggtt ttgcagatct tctatagaaa actttttaaa atcaagtaaa tggttaaccg
     4201 cctgtgccag tgtttttgcg gtagtttctc ctgcaaagcg tatacctagc gcataaataa
     4261 gccggtgaag cggttgcttt tttgaattct caatggctgc atgtaaattt tcaattgatt
     4321 tttggccaaa accttccatg ccgctgagag cggcaaaatc cagtgtataa attccgggaa
     4381 tgtcttttag taatcccagc tcataaaatt tacggacatt agcttcgcca aaaccacgaa
     4441 tatccatggc atctttactt acaaagtgaa tgatcctttc aacaacctgt gcagggcaat
     4501 caatattaat gcagcgccat acggcctctt cctcttcttt aaatagtttg ctgttgcata
     4561 caggacatgt tttaggaaag tgaatctttt tttcatgccc ggttctggta tctgccagtg
     4621 gtttcacaat ttgcggaata acatcaccgg cccgttcaat taacacggaa tcacctatgc
     4681 gcagatcttt ttctttaatg taatcttcat tgtgaacaga tatactgctg acggtgacac
     4741 ctcctatggc taccggtttc agttttgcaa ccggggttac ggctcccgtt ctccccacct
     4801 ggaactcaac gcccaataat tccgtggttg cctgtcttgc tttgaattta taagcaatgg
     4861 cccaacgggg atggtgtgat gtcatgccca ttttatcctg caatgctgtg tcgtttactt
     4921 tgatcaccat gccgtcaatt tcataaggca agtcatctct tccttcttca aattcattac
     4981 agtacttaat tactttttca attcctttga agaccttttt ttccttttcg gggcttcgga
     5041 acccaagatt ccatagtaag gccaatgagt cagaatgggt tgaaagctca accggtgtct
     5101 ttattccttt ttcagttgta tagaaactaa cgtggtaaac gaatgcttcc aggtttcttt
     5161 tgcctacttc aacagtgtct ttaatacgaa gtgttcccgc agcagcgttc cgtggattgg
     5221 caagcggcgg taaaccttgt tcagcaagtt gctcgttata ttttgcaaaa ttctttttat
     5281 tgatcaatac ttcccctcgt atttcaacct gtgttatacc atattctgaa aattttgccg
     5341 agaggggtat acttctgatc tgtttaatat taatagtgat atcatctccc tccacaccat
     5401 cgccccttgt ggcacccctt actaaaagat cattttcgta aataagcgaa atgctgctgc
     5461 catcaaattt tggttcaaca caatattcta tttcttttaa gcccgtcaat tctcttgcct
     5521 tcctgtcgaa atcaagaagg tcatcgctat tataggagtt atccagtgat aacataggta
     5581 caaggtgctg gaccgtagga aagtcttttg taagtccctt tgccactctt tgagttggcg
     5641 aatccggcat tttcagggta ggagtttcct gttcaatttt ttccaggcat ttgaataact
     5701 ggtcgtattc gtaatcagcg atcaaaggat cattaagtat atagtaccgg tattcatgga
     5761 accgtaatac atcgccaagt tcctgtacat tcttgctttt tatttgacct gccttcagtt
     5821 tagtaagcaa ttcattagta agcttttgaa gttggtgagt tgtttccttt gaatacataa
     5881 cagagaattt gaatactgtg ccagtaaagg taggaagcct gcttaaactt aagtagagac
     5941 aaaaacagac aaaaatttaa tgtgttataa gtacatatta cgtatcttcg aaaacaggtt
     6001 gctttacaaa acgatttgtg ttaaaataaa tgcttgctat gaaaaaagtt tttattgtct
     6061 gttttttaat gctgttattt tccttttcag gagatgcaca agtgcttacc tggactccgg
     6121 cattcccaaa ggataatgat a
//
LOCUS       DAORLS010000185         6110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_6995_length_6110_cov_2.242609, whole genome shotgun sequence.
ACCESSION   DAORLS010000185 DAORLS010000000
VERSION     DAORLS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6995_length_6110_cov_2.242609"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK977_13225"
     CDS             <1..700
                     /locus_tag="PK977_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRF19128.1"
                     /translation="LVNAGKKVGVICVDPSSPFNLGALLGDRIRMNEWYTHPGVFIRS
                     LATRGSMGGLHPKIIEISDLLKAFPFDHIIVETVGVGQSEIEIAGLADVTVVVVVPEA
                     GDEVQTMKAGLMEIADIFVVNKADRPDADLFVKNLRQMLAPSFSKHYHEVPVVKTVAS
                     QNSGVKELNEILLKLLQKSHLTDKTFWLLAERAFYLIEQKRMKDIDKAVLKKEIEKLY
                     PKGNFNLYKYISDK"
     gene            706..996
                     /locus_tag="PK977_13230"
     CDS             706..996
                     /locus_tag="PK977_13230"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRF19129.1"
                     /translation="METIPNEGFPRVAKCEPAGIMVEPGKVYSWCSCGLSEKEPFCDS
                     AHKKIEGMPFRSVKVTFDKAEEVWFCQCKQTKTPPFCDGSHKKVQSSINKGG"
     gene            996..1868
                     /locus_tag="PK977_13235"
     CDS             996..1868
                     /locus_tag="PK977_13235"
                     /inference="COORDINATES: protein motif:HMM:NF016602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19130.1"
                     /translation="MKIFDCFTFFNELDLLEFRLKFLDKHIDYFVIAESNITHSGQSK
                     PYYFPEAKDRFRQWEGKIIYIPVRQSTEGLVFEKQNKYNPGSAAWKLENDQRNALLQA
                     MHLMSDNDLVLLSDLDEIPLPAAIKKAKHHNKPVAFSLLFHYYFLNCQNTGESRWWKG
                     CIASTAKQFKEITPQGLRDKRDSYPSLPHAGWHFSFLGGAEKIRQKLLAFAHTEFNKD
                     EFTNEEHIKEAILKGEDILKREGVSFRYVPLSYYPATLQRIMKEYPELLYPKKKNFLA
                     DCYFTARRIVKGTF"
     gene            1870..2811
                     /locus_tag="PK977_13240"
     CDS             1870..2811
                     /locus_tag="PK977_13240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19131.1"
                     /translation="MTNTETIQPLVSIALCTYNGAAYIKQQLDSITDQTYTNLEIIIC
                     DDESVDDTIKIIEEYRAKEPRIKFFRNDKNIGYNKNFEKAFTLCSGDYIAISDQDDVW
                     EKEKIELMMKNWPSGSLFVYSLSGNFMNDDFSGRTEAPKVRYTAIDDLHKLVFNSPVH
                     GHACMFKKELLQLCLPFPPDIYYDWWMSMHAAAAGTIGCIPLTLTWHRNHSQNSSRTL
                     TTIKDREERDKQLRNQMIHFIETFCHSNRTNNKEKDSLLTYTRILKRMDGKKFNTDMF
                     FYVLKKRKLIFHYKKNNAFAFLSHLKHALRMGYRGLL"
     assembly_gap    2857..3027
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3262..3295
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3296..4242)
                     /locus_tag="PK977_13245"
     CDS             complement(<3296..4242)
                     /locus_tag="PK977_13245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF19132.1"
                     /translation="MKIGFAGRWSPLDKKSWSGTYYFSYNEIKKYGEVEIFHFPYPKW
                     LSTYLINVYKKPQNLLRRNVAVEFLKDYARHYSHKLEKELLRRKVDILFVASAPQLFA
                     YADIDIPVIFLTDGTFYQIQGYYDTWKNFAPFSIRQGIEVDKRAFLKATHIMAASDWA
                     KESAIRDYSVASENISVVPFGANLDYIPAADQLDFQASQQCRLLFFGVEWTRKGGDIA
                     LEAYWRLKQGGVNAKLLIVGCVPPAPIDDPDVTVIPYLDKNNPTDFERINDILLNTDF
                     MLLPTRAECAGIVFCEASSYGIPSVTTDTGGVSTYIKNGV"
     gene            complement(4260..>4894)
                     /locus_tag="PK977_13250"
     CDS             complement(4260..>4894)
                     /locus_tag="PK977_13250"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19133.1"
                     /translation="LIAPDSFMGQPLYHVAHKADVIRLQMLKETGGIYLDLDTISVKP
                     LADLLSHSFVIGQELKPAYVPKNWRQRLKLKMGLKKKPSAGATGLCNAVLLSEKNSDF
                     VNHWLREYKTFRSKGRDKYWNEHSVMVPERLAALYPASISKLSPYAFHYPLYDAAGLR
                     AMFEEKREFPDAYLHHLWESFSWNDYLSKLTPDIVQQKETTYNLIARRFL"
     assembly_gap    4895..4905
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4906..5090)
                     /locus_tag="PK977_13255"
     CDS             complement(<4906..5090)
                     /locus_tag="PK977_13255"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19134.1"
                     /translation="MIPNILHFIFGMAEDFGGKSFSFAHYLSVKSAVELNKSDVAYFH
                     YQFEPEGEWWERAKPLLT"
     gene            complement(5087..5884)
                     /locus_tag="PK977_13260"
     CDS             complement(5087..5884)
                     /locus_tag="PK977_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HRF19135.1"
                     /translation="MPDIASETNDLLHQYFDKVLVMTVPRFKERHERVKERLAGINFE
                     FFFGADKNELDEKSIDHVYHYKPENTLAVRQVFPALNKGEIACSLSHRMIYQAMLDNN
                     WQRVLILEDDVVPDYTTLPMLANCLKELPADWELFYLGYLKNEKATFGKKLKIFWYKI
                     MAMFGMSRMPASMLRNVLPRPFSSCLMKAGFHDCTHAYAISLEGAKKLLEAQTPVQFR
                     ADNLLSALVLKRKLNAFISKSNLFNQEIFSDITNKSYVRKDPKLNTE"
BASE COUNT         1815 a         1235 c         1193 g         1651 t
ORIGIN      
        1 cctggtcaat gcaggaaaaa aagtgggtgt tatttgtgta gacccttcat caccatttaa
       61 cctgggtgct ttgctgggcg accggatccg gatgaatgaa tggtacaccc atcccggggt
      121 ttttatccgt tcgctggcaa cccgcggttc catgggtggc ctgcatccca agatcattga
      181 aataagtgat ctgctaaaag catttccgtt tgatcatatc attgttgaaa cagttggggt
      241 tggacagagc gaaattgaaa ttgcagggct ggccgatgta actgtcgtag ttgtagtacc
      301 agaagctggt gacgaagtgc aaactatgaa agcggggcta atggaaatag ccgacatatt
      361 tgtagtgaac aaagctgaca ggcctgatgc agacctcttt gttaaaaacc tgcggcaaat
      421 gctggcacca tcgttcagta aacattatca tgaagtacct gttgtaaaaa ccgtagcgtc
      481 tcaaaacagc ggcgtgaaag aactaaacga aatacttctg aaattgctcc agaaatcaca
      541 tttaaccgat aaaaccttct ggctgctggc agaaagggct ttctacctga tcgaacaaaa
      601 aaggatgaaa gacattgata aggctgttct gaaaaaagaa attgaaaaat tatatccaaa
      661 aggaaatttc aatctatata aatacatatc agataaataa caactatgga aacaataccg
      721 aacgaaggat ttccgagagt ggcaaaatgt gaaccagccg gcattatggt agaacctggc
      781 aaagtgtatt cctggtgcag ttgcggtctt tctgaaaaag aacctttctg cgacagtgcc
      841 cacaaaaaaa ttgaagggat gccttttaga agtgtaaagg tgacatttga caaggcagaa
      901 gaagtgtggt tttgccagtg caagcaaaca aagacaccgc cattttgtga tggaagccat
      961 aaaaaagttc aatccagtat aaacaagggc ggctaatgaa aatatttgac tgctttactt
     1021 tcttcaatga gcttgacctg ctggaatttc gattaaaatt cctcgataag catattgact
     1081 actttgtcat tgcagaatca aatattaccc attcaggaca aagcaaacct tattattttc
     1141 cggaagcaaa agaccggttc cggcagtggg agggtaagat catttacatt cctgtaaggc
     1201 aaagtacgga gggccttgtg tttgaaaaac agaataaata caacccgggt agcgctgcat
     1261 ggaagctgga aaatgatcag cgtaatgcac tgctgcaagc aatgcacctg atgagtgaca
     1321 atgacctggt gctgctcagt gatctcgatg agataccatt accggctgct ataaagaaag
     1381 caaaacacca taacaagccg gttgctttct ccctgctttt tcattattac tttttaaact
     1441 gccaaaacac cggtgaaagc cgttggtgga aaggttgtat tgcgtcaact gcaaagcagt
     1501 tcaaagaaat aacaccacag ggtttaaggg ataaacggga cagctatcca tcactcccgc
     1561 atgccggctg gcatttttct tttttgggcg gggcggaaaa gatccgacaa aaattattgg
     1621 catttgcaca tacagaattt aacaaagacg agtttaccaa tgaggaacat ataaaagaag
     1681 ccatccttaa aggtgaagat atactgaaaa gggagggagt ttctttcagg tatgttcctt
     1741 taagctatta cccggccacc ctgcaaagaa taatgaaaga atatccggaa ttgctgtatc
     1801 caaaaaaaaa gaactttctg gcagattgtt attttactgc ccgtcgaatt gtaaaaggaa
     1861 ctttctgaat tgacaaatac agaaacgata caacccctcg tatcaatagc gctttgtaca
     1921 tacaatggtg cggcctatat aaaacagcaa ctagattcaa tcactgatca aacttatact
     1981 aatcttgaaa taatcatctg cgatgacgaa tctgttgatg acacaataaa gattattgag
     2041 gaataccggg caaaagagcc tcgtataaag tttttcagga atgataagaa tataggctac
     2101 aataaaaatt tcgaaaaagc atttacgctt tgcagcggag attatatcgc catcagcgac
     2161 caggatgatg tgtgggaaaa agaaaagatt gaactgatga tgaaaaactg gccttccggt
     2221 tcattgttcg tttattctct ttccggcaat tttatgaatg atgatttttc aggaagaaca
     2281 gaagcgccaa aggtccggta tacagctatt gacgatcttc ataaactggt tttcaatagc
     2341 ccggttcatg gtcatgcctg tatgttcaaa aaagagctgc tgcagctttg tttaccattt
     2401 cctcctgata tttattacga ctggtggatg agtatgcatg cagcggctgc tggtactata
     2461 ggttgtattc ctcttacatt aacatggcac agaaatcatt cacagaacag ttcccgtacc
     2521 cttacaacaa tcaaagacag ggaagaacgg gacaagcagc ttcgaaacca gatgatccat
     2581 ttcattgaaa ctttctgtca ttccaatcgg acaaataata aagaaaagga ttccttactt
     2641 acctatacca ggatattaaa aagaatggac ggaaagaagt ttaacaccga catgttcttt
     2701 tatgtgctga aaaaaaggaa actcattttt cactataaaa aaaataatgc ttttgctttc
     2761 ctgtcgcacc tgaagcatgc cttgagaatg ggttacaggg gattactata gattattagg
     2821 caggctaatc aaccttgtta tttttccacc agcgaannnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa aactgatttc aaaacccacc ttctcattgc
     3061 ctgaaaaatt tcagcaaatg tacagttctt ttagggatca gacagcagcc tgtttaaggc
     3121 aggcctgggc aattttataa aaactttcac cccatgcctc ccaggaaaga gtctcatcgt
     3181 aataagccct tgtactgtgc ttgaggtcaa tcaataattg aggattactt atgatttttt
     3241 ttatgcaatc cgcatattcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactcc
     3301 attttttata taggtactta cgccccccgt atcagtagta acagaaggta tcccgtagga
     3361 agaggcttca caaaaaacta ttccggcaca ttccgcccgg gtaggaagca gcatgaaatc
     3421 ggtgtttaat aaaatatcat taatccgttc aaagtctgtc gggttatttt tatccaggta
     3481 tggtataaca gttacatcag gatcatcaat tggggccgga ggcacacaac caacaatcaa
     3541 cagctttgca ttaactcccc cctgttttaa cctccagtag gcctcaagag caatatctcc
     3601 gccttttctt gtccactcta caccaaaaaa cagtaagcgg cattgttggc tggcttgaaa
     3661 gtcgagttga tcagcagccg gaatgtaatc aagattggcc ccaaaaggta ccactgaaat
     3721 attctcggaa gcaacgctat aatccctgat cgcagattct tttgcccagt ctgaagccgc
     3781 catgatatgt gtagctttaa gaaatgctct tttatctact tcaatcccct gcctgatact
     3841 gaagggtgcg aaatttttcc aggtatcata gtatccctga atctggtaaa aagtaccatc
     3901 ggtcagaaaa ataacaggga tatcaatatc cgcataagca aaaagctgcg gtgcagaagc
     3961 cacaaaaagt atatctactt tacggcgaag caattccttt tccagtttat ggctgtaatg
     4021 ccgggcatag tctttcagaa attcaacagc cacatttctt cgcaaaagat tctgcggctt
     4081 tttataaaca ttgatcaggt aagttgacag ccacttagga tagggaaagt gaaatatttc
     4141 aacttcaccg tattttttta tttcgttgta gctgaaataa taggttccgc tccaggattt
     4201 tttgtccagg ggactccatc tgcctgcaaa acctattttc atttgaggtt atttactttc
     4261 taaagaaacc ttctggctat aagattataa gttgtttcct tttgctgcac aatatcggga
     4321 gttagtttcg aaaggtaatc attccatgaa aaagattccc agagatggtg caggtatgca
     4381 tcgggaaatt cccttttttc ttcaaacatg gcccgaagac ctgctgcatc atacaaagga
     4441 taatgaaagg cataggggct aagtttggag atactcgccg ggtatagagc ggccagcctt
     4501 tccggcacca tcactgagtg ctcattccag tacttatccc ttcccttgga ccgaaacgtc
     4561 ttgtattccc tcagccagtg attaacaaaa tcactgttct tttcactcag cagtacggca
     4621 ttacataggc cggtggcccc cgctgaaggt ttctttttca gtcccatttt caatttcaat
     4681 cgctggcgcc agtttttagg aacatatgcc ggttttaatt cctgtccaat cacaaatgaa
     4741 tgactaagaa gatcagccag gggtttaacg ctgatagtgt cgaggtcaag ataaatcccc
     4801 ccggtttctt tgagcatttg caaacgtatt acatctgcct tatgggcaac atggtacaaa
     4861 ggctgaccca taaaagaatc aggggctatt aattnnnnnn nnnnngtcag taatggtttg
     4921 gcccgttccc accattctcc ttctggttca aactggtagt ggaaataagc tacatcggat
     4981 ttattcagct ctacagcaga cttaacagac aaataatgag caaacgaaaa agacttacct
     5041 ccgaaatctt cggccattcc aaaaataaaa tgaagaatat tgggaatcat tcggtattaa
     5101 gctttggatc tttacgaacg taggatttgt tggtaatatc ggagaaaatt tcctgattga
     5161 acaaattaga tttactgata aaggcattca gctttctctt cagcactaat gccgacaaca
     5221 ggttatcggc cctgaattga accggggttt gtgcttccag tagttttttg gcaccttcca
     5281 aactgattgc atatgcatgg gtgcaatcat gaaagccagc cttcatgaga caactgctga
     5341 atggccttgg taatacatta cggagcattg aagcaggcat ccggctcata ccaaacatcg
     5401 ccattatctt ataccagaat attttcagct ttttaccgaa ggttgccttt tcatttttta
     5461 agtagccgag gtaaaagagc tcccagtcag ccggtaattc ctttaagcaa ttggccagca
     5521 tgggcagtgt tgtataatcc ggcactacat catcttccaa tatcagcacc cgctgccagt
     5581 tattatccag catagcctgg tagatcatcc ggtgagaaag tgaacaggct atttctcctt
     5641 tgttcagcgc tggaaaaacc tgcctgacag ctaatgtgtt ttcaggtttg taatgataaa
     5701 catgatcaat agatttttca tccagctcat ttttatcagc accaaaaaag aattcgaaat
     5761 tgattccggc cagtctttct ttaacccttt catgcctttc tttaaaacgg gggacagtca
     5821 tcaccagcac tttatcaaag tattgatgta aaagatcgtt ggtttcagaa gctatgtccg
     5881 gcatgaagac aaatgtaaaa aaacagcaaa tacccgaaag tatttgctgt aataaataaa
     5941 ttaatccttc taataattaa accgcatccg cctccatctt cttctgcact ttctttctct
     6001 cctcctcatt cagtatcact ttacgcatcc tgataaaatt gggggttact tcaatacact
     6061 catcctgctg gatatattcc atgcattcct ccagtgtcat cagtgtcttg
//
LOCUS       DAORLS010000186         6102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7009_length_6102_cov_1.476104, whole genome shotgun sequence.
ACCESSION   DAORLS010000186 DAORLS010000000
VERSION     DAORLS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7009_length_6102_cov_1.476104"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>978
                     /locus_tag="PK977_13265"
     CDS             <1..>978
                     /locus_tag="PK977_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19136.1"
                     /translation="TAQLASVPYSLYSNLSGDLVLPFNKTQADAGTLFKITNSNTTSA
                     GATALEGLTNSTAGNANAIIGTVTSASPGGFSAGVRGINNGTGGLGIGVYGSQAGSGW
                     GVYGFTPGGIGVNGAATTGTGLWGSSNSGTGVFGTSSTGSAGVFSNTNAANNLPTVAI
                     STNSTNEPALRVTGPNIWGSSIAINNSVSGMEWRMNVQGTTWQVTKVPGSTFTPFRLF
                     QNGNVDFPNTAGTSRVLLLDNGDMGVGTTTPFARLTVVQNNSGVNLGGGANTGSEIKF
                     LGFGTSHISIYNRGNNALTFANTSALAQTDVLGSPLMTLTTAGRLGIGTT"
     assembly_gap    979..1141
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1142..>1443
                     /locus_tag="PK977_13270"
     CDS             <1142..>1443
                     /locus_tag="PK977_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19137.1"
                     /translation="TLNFVPKWTPNGTNLGNSLLFDDGTRMGIATTTPAYKLDVDATT
                     GGGNGIRVRNTGSFATVDIIGAGNDQALRFGKAGSLYSGINTLPDGDHINFFKFGAG"
     assembly_gap    1444..1453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1460..>2615
                     /locus_tag="PK977_13275"
     CDS             1460..>2615
                     /locus_tag="PK977_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19138.1"
                     /translation="MSLNVNTGNLGVGGNPSAINAVGKVEVRGFNEANNNYTVASGGY
                     QGVTGLVSRDETSAGMPIKIGSYSYADGATSFNFGYNAETGSSAQFNYGAYLRVSSAP
                     PPANTAVSLYASDLVGAVNTYAAWFNGKLRYQDGNQGAGKILVSDAVGDASWSTTSGA
                     GIVGGSGTLNFVPKWTPDGNTLGNSQMFDDGQTTNIGGGALGNRPSTLTLWQSPGTTQ
                     ASLLFRSPIGELNGIHNEDDGKLYFTANTSDPLTANKIMTIDDGILGVGIGTSNPTTR
                     LNVVQTDATAIFSEVNYTAGVPSIFDDRAANMINSTNSNFGLAAFNNHSNALIPGWKA
                     AVMGIDSSNDIFSSGVAGLYKGNSAGIGVYGASAQNRAFESIATTSALGYV"
     assembly_gap    2616..2778
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2779..3318
                     /locus_tag="PK977_13280"
     CDS             <2779..3318
                     /locus_tag="PK977_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19139.1"
                     /translation="GFFQGSIAVVDGTEGAGRVLTSDASGNASWQDLITPAVGISLAD
                     LTVPQSIPSGVGTDVNTWAAALYEDGGANFNPVTGEYTITVTGLYTINGSIGWSSFGA
                     AASEVLTRVLVNGVVVARGFSNQGIVGEYPSTSQATYTTRLNAGDIVKLQAFQNSGSP
                     VTLSSSNRETRFTVTLLRR"
     gene            3346..3702
                     /locus_tag="PK977_13285"
     CDS             3346..3702
                     /locus_tag="PK977_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19140.1"
                     /translation="MKKILILFVAAVISSVICIGQEKENAATTNSRATELMINGKPYS
                     QYKAEQEALKQIKANQAAATKVATTNLSNADVTKTPMAESPTKPAVKEVNQASDDQRE
                     AAPGGFNTTCKNLYFS"
     gene            complement(3981..4709)
                     /locus_tag="PK977_13290"
     CDS             complement(3981..4709)
                     /locus_tag="PK977_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF19141.1"
                     /translation="MKKTISFLIGIVCVTAVTAQDTEAMKKHYLKMYDNALVYNDARA
                     AINALHGYVAIDSGAAYKDTLSMLYFSTKAYVSALILAEEVYKKVPANTEAMARAAEC
                     YDELGDPKTAVGLYEQVVPKTKNPYHIYKLGVGQYQLKRTLECEVSARAVLADTTSKR
                     IGVNFTSIDGSQQAVPVNAAAANLLGVLKMDAKNYAGAKTDFQEALKMYPDFAGAKQN
                     LEACEKALKGGTKAPAKTPTKPKG"
     gene            4816..>5135
                     /locus_tag="PK977_13295"
     CDS             4816..>5135
                     /locus_tag="PK977_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19142.1"
                     /translation="MRCISVFLLSICLFCTLTPEAQQDSIDTERLAKMVSLSEVIIRS
                     DMNVARFIDQVKKDTTFYKAFKNLRVLGFTSWNDIRIKDKKGAIKAALLSKTRQLRSS
                     GCRS"
     assembly_gap    5136..5291
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5292..>5673
                     /locus_tag="PK977_13300"
     CDS             <5292..>5673
                     /locus_tag="PK977_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19143.1"
                     /translation="LAKHKEQLKMLFFNPGKKIPGIPFIGDKINIFDPDKAVLYDFLV
                     DFGDYEGQSCYVFTIRSKEGLSNSERNDVVIDSMVTYFNTKTMEVMARRYDMSFDAGV
                     YDFNVHMEVEMTKFGNYLVPRLLRY"
     assembly_gap    5674..5759
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5770..>6102)
                     /locus_tag="PK977_13305"
     CDS             complement(5770..>6102)
                     /locus_tag="PK977_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRF19144.1"
                     /translation="RFGYVTLVECILETGRTHQIRVHMKHIGHPLFNDDFYGGDKIVK
                     GTVYSKYKQFVENCFQVCRRQALHAKTLGFVHPSTGENMFFDTALPEDMAEVIEKWRK
                     YTEATGNR"
BASE COUNT         1547 a         1110 c         1246 g         1621 t
ORIGIN      
        1 aacagcccaa ctggcttctg ttccttattc tttatacagc aatctttccg gagatctggt
       61 gttgccgttt aataaaacac aggcggatgc cggtacattg tttaaaataa caaatagcaa
      121 cacaacatca gcaggcgcca ctgcacttga aggtttaacc aacagtacag ccggcaatgc
      181 caatgccatc attggtacgg taacctctgc atcaccgggt ggcttttctg caggtgtaag
      241 aggtatcaat aacggtaccg gcggtttagg aattggtgta tatggttcac aagccggcag
      301 cggctggggt gtatatggat ttaccccggg cggtatcggt gtaaatggtg ctgctactac
      361 cggaactggt ttatggggta gctctaatag tggtactggt gtatttggaa ctagcagtac
      421 gggtagtgcc ggtgtattta gtaataccaa tgctgccaat aatcttccta ctgtagctat
      481 tagtaccaat tcaaccaatg agccggccct cagggtaact ggccctaata tctggggtag
      541 ttcaattgct attaataact cagtttcagg catggagtgg agaatgaatg tacagggtac
      601 aacctggcaa gtaaccaaag tacccggttc cacatttact cctttcagat tgtttcaaaa
      661 tggaaatgta gattttccaa atactgcagg tacatcaaga gttttgttgc ttgataatgg
      721 agacatgggt gtaggtacta caacaccatt tgctagatta acagttgtac aaaataactc
      781 tggtgttaat cttggtggcg gagcaaacac cggctcagaa attaaattcc ttggttttgg
      841 aacaagtcat atcagtattt acaaccgggg aaataatgca ttgacatttg ccaataccag
      901 tgctttagcg caaacagatg tgttaggctc cccgttaatg accttaacta ctgcaggcag
      961 gttaggtata gggacaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nactttgaat tttgttccta aatggacgcc gaatggtact aaccttggta attcattgct
     1201 gtttgatgat ggtaccagga tgggtattgc tactaccacg ccggcttata agctggatgt
     1261 ggacgctact accggtggtg gtaatgggat aagggtacgc aataccggca gttttgctac
     1321 tgtggacatt ataggtgctg gaaatgacca ggctttacgt tttggtaaag cagggtcact
     1381 ttattctggt attaacacat tgcctgatgg tgaccatatt aattttttca aatttggtgc
     1441 cggnnnnnnn nnnaatttga tgtctctgaa tgtaaacaca ggtaaccttg gagtgggtgg
     1501 aaacccttct gctatcaatg ctgtgggcaa ggtagaggtt agaggtttta atgaagcaaa
     1561 taataattat acagtcgcca gtggtggata ccagggtgtt actggtttag tttcccgtga
     1621 tgaaacatca gcaggtatgc ctataaaaat cggttcctat tcctatgccg atggtgccac
     1681 tagtttcaat tttgggtata atgcagaaac aggttcatct gcgcaattca actacggggc
     1741 ctatttacgg gtaagttctg ctcctcctcc ggccaatact gcagtgtctt tgtatgcttc
     1801 tgatctcgtt ggcgctgtta atacttacgc agcctggttc aatggaaaat tgcgttacca
     1861 ggatggcaac cagggtgccg gaaaaatact tgtttcagat gctgttggtg atgccagttg
     1921 gagtacaact tccggagcag gtattgtggg tggaagcggt acattaaatt ttgttccaaa
     1981 gtggacaccg gatggaaata ccttaggtaa ttcccaaatg tttgacgatg gtcaaactac
     2041 caatataggt ggaggtgcac ttggaaacag gcccagcaca ttgactttgt ggcagagtcc
     2101 cggtacaaca caggcatctc ttttgttccg ttcaccgatt ggggagttaa atggtataca
     2161 taacgaggat gatggcaaac tctattttac tgcaaacaca agtgatccat taacagcaaa
     2221 caaaatcatg accattgatg atggtattct tggtgttggt attggtacta gtaacccaac
     2281 aacaagatta aatgtagtac aaacagatgc tactgctatt ttttctgagg ttaattatac
     2341 agcgggtgta ccaagtatat ttgatgacag ggcagccaat atgattaatt caacaaattc
     2401 caattttgga ttggctgcat ttaataatca ctcaaatgcc ctgatacctg gctggaaggc
     2461 agcagtaatg ggtattgact caagtaatga tatcttctca agcggtgtag caggccttta
     2521 caaagggaat agtgcaggta ttggtgttta tggtgcttct gctcaaaaca gggcgtttga
     2581 atcaattgca acgacttctg cattaggata tgttgnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnngg tttcttccag ggtagtattg ctgttgtaga tggtactgaa
     2821 ggagcaggta gggtactaac cagtgatgca agtggtaatg caagctggca ggatttgatt
     2881 acacctgcgg tcggcattag tttagctgat ctaactgttc ctcaaagtat tccttcagga
     2941 gtaggcacag atgtgaatac atgggctgct gcattgtatg aagatggcgg agctaatttt
     3001 aatcctgtga ccggtgaata cactattact gttactggtt tgtatactat taatggatca
     3061 ataggctgga gttcttttgg tgctgccgca tctgaagtat taacaagagt tcttgtgaat
     3121 ggagttgtgg tagcaagggg gttctcaaat cagggtatag taggggagta tccatccacc
     3181 agccaagcta catatacaac aaggcttaac gctggagata ttgtaaaact tcaggctttc
     3241 caaaattctg gttcacccgt tacccttagt tcatcaaata gagaaaccag atttactgtg
     3301 accttgttaa gaagataatt tttgacatat ttaaaattaa ttattatgaa aaaaatatta
     3361 atactgtttg ttgctgcggt gatttcctcg gtgatttgta taggccagga aaaagaaaat
     3421 gctgccacaa caaattcaag agcaacagag ttgatgataa acggcaagcc ctacagccag
     3481 tataaagctg aacaagaggc tttaaaacaa ataaaggcca accaggcagc agcgacaaaa
     3541 gttgcaacaa ccaatctttc caatgcagat gtaacaaaga caccgatggc agaatctcca
     3601 acaaaacctg ccgtaaaaga agtaaaccag gctagtgacg atcagcggga agcggcccca
     3661 ggtggtttca acacaacctg taaaaacctc tacttctcct gaaaataaag ttgtaccatc
     3721 tgcagcaaaa aacagctctg ctgacccaga tgcaaaatct gttgcaccag caaagcaaac
     3781 atctatttcg gctgcggttc cggctcaaaa ctccgggaac accggtcctg cagcagagaa
     3841 acctgcaacc aaagattaat tctgtttcta acagaaaaaa atgagaaggc tttcgggcct
     3901 tctttttttt gcctggtact acaaaaaaga aagaagacca ttttggtctt ctttcaatat
     3961 acttattgac ttattaaata ctatcccttt ggcttagtcg gcgttttggc tggtgccttt
     4021 gttccacctt ttaatgcttt ttcacaagct tccaggtttt gtttggctcc tgcaaaatca
     4081 ggatacattt ttaatgcttc ctggaaatct gttttagcac ctgcataatt tttggcatcc
     4141 atttttaata caccaagtaa attagccgcc gctgcattta ccggcaccgc ctgctggctg
     4201 ccatcaatag aagtaaagtt tacaccaatt cgtttgcttg tcgtatcagc cagtactgcc
     4261 ctggcagata cctcacattc caatgttctt tttaactggt attggccaac ccccaattta
     4321 taaatgtgat aggggttttt tgttttaggt acaacctgtt catacaagcc aacagccgtt
     4381 ttgggatcgc ccagttcatc atagcattcg gcggcacggg ccatggcttc tgtattggcg
     4441 ggtacttttt tatacacttc ttctgccagt atcaaagcac ttacataggc ttttgttgaa
     4501 aaatagagca tcgataaagt atccttgtag gctgctccgc tgtcaatggc cacatacccg
     4561 tgcaaggcat tgatagccgc tcttgcatcg ttatacacaa gtgcattatc gtacattttc
     4621 agatagtgct ttttcatggc ttctgtatcc tgtgctgtta cagcagtaac gcaaactata
     4681 ccaattagaa atgagattgt ttttttcatc attaaaggtt tttgattagc acctgacttc
     4741 aagcaggcgg ggttaaaaat aagcgttccg gttgaaataa aggccgattt acagttcaaa
     4801 aaactaattt gcggtatgcg ttgtatatca gtgttcctgc tcagcatctg cctgttttgt
     4861 acactcacac cggaggcaca gcaggattcg attgacacag aacgcctggc aaaaatggtc
     4921 agcctttcag aagtgattat ccgctctgat atgaatgtgg cccggtttat tgaccaggtt
     4981 aaaaaggaca ccacttttta caaagctttc aaaaatctca gggtattggg ttttacttcc
     5041 tggaatgata tccgcattaa ggacaaaaaa ggggctatta aggctgcatt attgagtaag
     5101 accaggcagc ttcgttcgag cggctgtcgt tcaatnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nccttgccaa acataaggag caattgaaga tgctgttctt taaccccgga
     5341 aaaaagatac cgggtattcc gtttatagga gataagatca acatcttcga tccggacaaa
     5401 gctgtgctgt atgatttcct tgttgacttt ggcgactatg aaggacagtc ttgttatgtg
     5461 tttactatcc ggtcaaaaga agggttaagt aacagtgaaa ggaatgatgt tgttattgac
     5521 agcatggtca cttatttcaa cacgaagaca atggaagtga tggcccgtcg ctacgatatg
     5581 agttttgatg ccggtgtata cgattttaat gtacacatgg aagtggaaat gacaaagttt
     5641 ggcaattacc tggtgcccag gctccttcgt tatnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5761 caattaagct tacctgttcc ctgttgcttc ggtgtatttt ctccattttt caataacctc
     5821 tgccatgtct tccggcagtg ctgtatcaaa aaacatgttt tccccggttg aaggatgtac
     5881 aaagcccagt gttttggcat gcagtgcctg ccgccggcat acctggaagc aattttctac
     5941 aaactgtttg tatttactat acacggtacc ttttacaatt ttatctccgc cataaaaatc
     6001 atcattgaac aggggatgac ctatatgttt catgtgcacc cttatctggt gggtacgacc
     6061 ggtttctagg atacattcta ccaaagtaac atagccaaac ct
//
LOCUS       DAORLS010000187         6094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7019_length_6094_cov_2.259977, whole genome shotgun sequence.
ACCESSION   DAORLS010000187 DAORLS010000000
VERSION     DAORLS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_6094_cov_2.259977"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PK977_13310"
     CDS             <1..352
                     /locus_tag="PK977_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRF19145.1"
                     /translation="GSITFAGVDTGLNHFIRPMFYGAYHEIENISNPSGEPKLYNVVG
                     NICETDTFADNRSLPEIREGDFLVFSNAGAYGFEMASNYNSRYRPAEVLFKNGLPQLI
                     RRRETFEDLLSNQI"
     gene            complement(361..1224)
                     /locus_tag="PK977_13315"
     CDS             complement(361..1224)
                     /locus_tag="PK977_13315"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HRF19146.1"
                     /translation="MKKNWITLACLLSATFAMGQKVAGKIKFEQGQSLNVTMQMKTTI
                     AQQAMGQAIDFTVDATGIHEYKVTNTTDDNSTLNHQMRSVNFAFDGMGQKRNFDSNNE
                     KDISGPFGKPIKDLLDKKYDIIIDPSGTTMMALPEKIQLTENDSRMAIISNMMKDVFD
                     LVQPPKQGRASFFKVLPESEVGIGDTWTESYDLEGGKYSATYKLAEVTDSLIVIDFTA
                     NSVTVSKAEMMGNETTTTMNNKSTGKILVDKNTGIIREKTINTESTGNTETSFGNLPV
                     TSRTSTIITVK"
     assembly_gap    1451..1460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1461..>1842
                     /locus_tag="PK977_13320"
     CDS             <1461..>1842
                     /locus_tag="PK977_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C1A papain"
                     /protein_id="HRF19147.1"
                     /translation="PGGGGGGLMSLLPMLMGLFKGKGIIFLLVLAAGAYFLLGRSGGC
                     NVADVVKKFSESGYNFNPAEFNKASVYEGLEDDAGKNPLPEAVSLLRFAPDRLNQGQQ
                     GSCVAWSCAYSAQTILTAAATGADP"
     assembly_gap    1843..1879
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1880..>2480
                     /locus_tag="PK977_13325"
     CDS             <1880..>2480
                     /locus_tag="PK977_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF19148.1"
                     /translation="LYNQIKLDGCQGSYLQRAMEAMSRNGGVPLSQYPYNDQDCDRDP
                     SSLYSAGRQNVIHGFTRITNGDNINEISVRGIKEHLAKNAPVAIGMMVGQSFMQDMMG
                     QELWTPQGMDESQMGMGGHAMTVIGYDDRKFGGAFQIMNSWGPEWGQNGVAWVRYGDF
                     KNYVREAYGIDPLPKRSNVANIPLECAIGLVNNEDKQNIT"
     assembly_gap    2481..2490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2491..2973
                     /locus_tag="PK977_13330"
     CDS             <2491..2973
                     /locus_tag="PK977_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRF19149.1"
                     /translation="AGGNLFETASPIKVGTRFKMSIENATECYIYVFGQEVDGSSFVL
                     FPYLKPGETVSKHSPYCGITGYRLFPKAQSMEADSIGTKDYIAIVVSKDELDYNSINN
                     AISQSRQTTYAAKVNEALRPVLISSAKFNNTNDGRIYFKVDANSNKAVAAIVAFDKRP
                     "
     gene            2993..3487
                     /locus_tag="PK977_13335"
     CDS             2993..3487
                     /locus_tag="PK977_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF19150.1"
                     /translation="MKRIIVVTLISFSSAMVYSQDMTKFRLYNPGDNAEKQIEAAVEK
                     AKDSGKHVMLQIGGNWCVWCARFNDFVTNDKSLDSLLNANYVVYHLNYSKENKNLDIL
                     KKYSFPQRFGFPVFLVLNEKGQLLHTQTSWYLEAGKGYDKEKVTAFFNDWGKKALDPA
                     QYND"
     gene            3490..3978
                     /locus_tag="PK977_13340"
     CDS             3490..3978
                     /locus_tag="PK977_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HRF19151.1"
                     /translation="MNSHTQTFIKLMKHPVKFRMFLFAKLPAAYMAGVRVREVDEKKC
                     VATVPYKWLSQNPFRSTYFACLSMAAEMSTGALAMAHLYRIHPPVSMLVVKVESEYFK
                     KATGRTSFICEDGELFQKAIEETISTGETRIVKAKSVGTNANGEIVAEFLITWSFRVK
                     TK"
     gene            4043..>4302
                     /locus_tag="PK977_13345"
     CDS             4043..>4302
                     /locus_tag="PK977_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF19152.1"
                     /translation="MKISFHGAARTVTGSKHLLTLKNGKKILLDCGMFQGMGKETDML
                     NRQFGFNPREIDVMVLSHAHIDHSGLIPKLIAEGFEGKIFCTP"
     assembly_gap    4303..4402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4403..5058
                     /locus_tag="PK977_13350"
     CDS             <4403..5058
                     /locus_tag="PK977_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF19153.1"
                     /translation="YLKPLYSVEDAAKAEEHFVVRAYNEWFDVTDGVQAMYTDAGHII
                     GSGCVHLRIKEKEEKEQRLTFSGDVGRYRDAILKSPETFPQADYIILESTYGNSLHDN
                     NVTTPDMLLRWIEKACLQKKGKLIMPAFSVGRTQELLYSLNQLEIENRLPPVNYYIDS
                     PLSVEATEIVKRYPQYFNKTIQRIMQNDQDPFSFEGLHYIKTVDQSKHEWPDGDHFG"
     gene            5027..5437
                     /locus_tag="PK977_13355"
     CDS             5027..5437
                     /locus_tag="PK977_13355"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HRF19154.1"
                     /translation="MNGPMVIISASGMADAGRVKHHISNSIENSHNTILLTGYCEPRS
                     LGGRLLAGEKEVGIFGVQHEVHAEIGVIRSMSAHGDYEDLCQWLACQDARQVKKLFLV
                     HGEYEVQQNFKQRLIKKGFLDVEIPEMHYEVGLG"
     gene            5554..>6094
                     /locus_tag="PK977_13360"
     CDS             5554..>6094
                     /locus_tag="PK977_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HRF19155.1"
                     /translation="MKKFRKLWSLPVAIVFLLASCTSTAHIEKDDNTDFSRYKTFAWI
                     EKDGQGNSNKIKNNNLTEQKIRQAVNAELIKTAGWREVKHKPDVMLSYDVLVERSSRE
                     INSPVYSRPFTRLIYNPYTRRYTTIYYPSQFVGYERDERAIREGTVTITMIDSRTDKA
                     VWQGWTTEEVNSKNLTTKEI"
BASE COUNT         1797 a         1089 c         1398 g         1653 t
ORIGIN      
        1 cggaagtata acttttgccg gtgtggatac cggtcttaat cattttattc gacctatgtt
       61 ttatggtgct taccatgaaa ttgaaaatat ctccaaccca tcaggcgaac ctaagttgta
      121 caatgtagta ggcaatattt gcgaaaccga cacatttgcc gataacaggt ctttgcccga
      181 gatcagggaa ggtgattttc ttgttttcag caatgcaggt gcctatggct ttgaaatggc
      241 atcaaattat aattcaaggt acaggccagc agaagtatta ttcaaaaatg gattaccgca
      301 actgatcaga aggcgggaaa catttgaaga ccttctttca aatcaaatat aaccgggcat
      361 ctattttacg gtgataatgg tactggtcct tgaggtgact ggtaaatttc caaaacttgt
      421 ttctgtattt ccggttgatt ctgtgttgat tgttttttca cggataatgc cggtattttt
      481 atctaccagg attttaccag tagatttatt gttcatcgtc gtagtagttt cattgcccat
      541 catttccgct ttacttaccg ttacagaatt ggccgtaaaa tcaatcacaa taaggctatc
      601 ggttacttca gcgagtttat aagtggcact gtacttcccg ccttcaaggt cgtatgattc
      661 tgtccaggta tcgccaatac caacttccga ttcaggcagc actttaaaga aactggctct
      721 tccttgtttg ggtggttgta ccaggtcaaa aacatctttc atcatattac tgatgattgc
      781 catccggctg tcattttcgg tcagttgaat tttctcagga agggccatca tagtagtgcc
      841 ggacggatca atgattatgt catatttctt atccagaagg tctttgatag gttttccgaa
      901 tggaccgctg atatcctttt cattgttgga atcgaaattt cttttttgcc ccatgccatc
      961 aaaagcaaaa ttgacggatc tcatctgatg gttgagtgta ctgttatcat cggtggtatt
     1021 ggtcactttg tattcatgaa tgcctgtggc atcgacagta aagtcaatgg cctggcccat
     1081 tgcctgctga gcaatagtgg ttttcatttg catggtcaca ttaagtgact ggccttgctc
     1141 aaattttatt tttcctgcca ctttttgtcc catagcaaaa gtggctgata acaagcaggc
     1201 taaagtgatc cagttttttt tcataatttt ttaaaataag aagcacaaaa atgacgatag
     1261 ttatttataa acgttgcagc atattaaata ttaacaacaa agattgtatt actgtttatt
     1321 cggctgaact tgtttatttt taatacaaat tactattaaa aaagtttact atgcctatca
     1381 gaatgacaga tgatcctgtg gatcctaacc agcaggatga cagcggtgga ggtggaggaa
     1441 gaagaccctc nnnnnnnnnn ttcctggcgg gggtggtggt ggtttgatgt ctttgcttcc
     1501 tatgctaatg ggattgttta aggggaaagg aattattttc cttttagtgc ttgctgccgg
     1561 ggcatatttc ctgcttggca ggagcggagg ctgcaatgtg gcggatgttg taaagaaatt
     1621 ttcagaaagc ggatacaatt ttaatcctgc tgaattcaat aaggcatctg tttatgaagg
     1681 actggaagat gatgccggga aaaatccttt accggaggca gtatcattat tacgatttgc
     1741 tccagaccgg ctaaaccagg gacagcaagg cagttgtgta gcgtggagct gtgcttatag
     1801 tgcacaaaca attttaacag cagcagcaac aggtgccgat ccnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnna tttatataac cagatcaaat tggatggctg ccagggctcc
     1921 tacctgcaaa gagccatgga ggcgatgagc agaaatggcg gggtgcccct aagtcaatat
     1981 ccctataatg accaggattg tgatagagat ccaagcagtt tatattcagc aggcagacaa
     2041 aatgtaatac acggatttac ccgcatcacc aacggtgata atatcaatga aattagtgtc
     2101 cgtggtatta aagagcattt ggctaaaaat gcgccggtgg ctattggcat gatggtgggc
     2161 caaagcttta tgcaggatat gatggggcag gaattgtgga ctcctcaggg aatggatgag
     2221 tcgcaaatgg gaatgggcgg tcatgcgatg accgttatcg gttatgatga tcgcaaattt
     2281 ggcggtgctt tccagatcat gaatagctgg ggacccgaat ggggacaaaa cggtgtggca
     2341 tgggttcgct acggtgattt taaaaactac gtaagggaag cgtatggtat cgacccgctt
     2401 ccaaaaagaa gcaatgtggc aaatattcca cttgagtgtg ctatcggtct ggtgaataat
     2461 gaagataagc agaacattac nnnnnnnnnn gctggtggaa atttatttga aactgcatct
     2521 cccataaaag tgggcacccg gtttaaaatg tcaatcgaga atgctacaga atgctacatc
     2581 tatgtattcg gacaggaagt ggatggtagt agttttgtgt tgtttcctta cctgaaaccc
     2641 ggcgaaacag tttcaaaaca ttcgccctat tgcggtatta ccggttatcg tttgttcccc
     2701 aaagcacaat ccatggaagc agacagcatt ggtactaaag attatattgc catcgtagtg
     2761 agtaaagatg aactggatta taacagtatt aataatgcga tcagccaaag ccgccaaacc
     2821 acttatgctg caaaagtgaa cgaagcgctt cggcctgttt tgatttcttc tgcaaagttc
     2881 aacaatacga atgatggcag gatttatttt aaagtagatg caaacagtaa caaggcagta
     2941 gcggctattg ttgcgtttga taaaagaccc taaaagtttt atataaagaa agatgaaaag
     3001 aattattgtt gttacgttga tatccttttc ttctgcgatg gtttattcgc aggacatgac
     3061 aaagttcagg ctgtacaacc ccggagataa tgcagaaaag caaattgaag cggcggtaga
     3121 aaaggcaaaa gattcaggaa agcatgttat gctgcagatc ggcggcaact ggtgtgtttg
     3181 gtgtgcccgg ttcaatgatt ttgttacaaa tgataagtca ttggactcct tgctgaatgc
     3241 aaactatgta gtgtatcacc tcaattacag caaggaaaac aagaaccttg atatactgaa
     3301 aaaatatagc tttccccagc gatttgggtt tcccgttttt ttggtgttaa atgaaaaagg
     3361 gcaattgctg catacacaga cttcctggta tttggaggca ggaaaaggtt acgataaaga
     3421 aaaagtgaca gcctttttta atgactgggg aaagaaagct ttagacccgg cacaatacaa
     3481 tgactgatca tgaattcaca tacgcaaaca tttattaagc tgatgaaaca tcctgttaag
     3541 ttcaggatgt ttctgtttgc caaattacct gctgcgtata tggccggtgt tagggtaaga
     3601 gaagtggatg aaaaaaaatg tgtggcaacg gtgccctata aatggctctc acaaaaccct
     3661 tttcggtcta cttattttgc atgtctcagc atggctgcag aaatgagtac aggagcactg
     3721 gcgatggctc atttatacag gatacatccg ccggtatcga tgctggtagt aaaggtggag
     3781 tcggaatatt tcaagaaagc cacaggcaga acaagtttta tttgtgagga cggtgagctg
     3841 tttcagaaag ccattgaaga aacaatttca acaggagaaa cccgaatcgt gaaagccaaa
     3901 tcggttggaa caaatgcaaa tggtgagata gtggcagaat ttttaataac ctggtcattc
     3961 agggtaaaga caaagtaatt ttttaatcaa aaaatccctt gggaggaacc atgaagttca
     4021 tccaaaggga ggaggagtgg gtatgaaaat ttcatttcat ggtgccgcca ggaccgttac
     4081 tggttctaag cacctgctga cattaaaaaa tggcaagaaa atattgcttg actgtggtat
     4141 gttccagggc atgggtaaag aaaccgatat gctcaacagg caatttggtt tcaaccctag
     4201 agaaatagat gtgatggtgc tatcccatgc acatatagac catagcggtt tgataccaaa
     4261 attgattgct gagggttttg aagggaagat attttgtacg ccnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nncctatttg aaaccacttt attctgttga agatgctgca
     4441 aaagccgaag aacattttgt agtaagagct tacaacgaat ggtttgatgt aacagatggg
     4501 gtgcaggcta tgtacactga tgcaggccat attatcggca gcggttgtgt gcatttgcgg
     4561 atcaaagaaa aagaggagaa agaacaaagg ctaactttca gtggagatgt gggacgttac
     4621 agggatgcaa tactgaagtc accagaaact tttccgcagg cagactatat tattcttgag
     4681 tccacttatg gcaatagtct tcacgataat aatgtaacaa caccggatat gctgctgcgc
     4741 tggattgaaa aggcctgcct gcaaaaaaaa gggaaactga tcatgccagc ctttagtgta
     4801 ggaagaacgc aggaactgct atactcattg aaccagttgg agatagagaa caggctgccg
     4861 cctgtgaatt actatatcga cagcccttta agtgtagaag ctacagagat tgtaaaacgc
     4921 tacccgcaat attttaataa gaccatacag cggatcatgc aaaatgacca ggatccattt
     4981 tcatttgaag gattgcatta tatcaaaaca gtagatcagt ccaaacatga atggcccgat
     5041 ggtgatcatt tcggctagtg gtatggctga tgcaggcagg gtgaagcacc atatcagcaa
     5101 cagtatagaa aacagtcata atacaatctt gctgactgga tattgtgagc cccgttccct
     5161 gggtggtcgt ttgctggccg gtgaaaaaga agtaggcatc ttcggtgtcc agcatgaagt
     5221 acatgcggag atcggtgtta tccgtagcat gagtgcccat ggcgactatg aagacctgtg
     5281 ccagtggctg gcctgccagg atgcccgcca ggtaaaaaaa ctatttctcg tacacggaga
     5341 atacgaagtg cagcaaaact ttaaacagcg gctaatcaaa aaaggattcc tggatgtgga
     5401 aatacccgaa atgcactacg aagttggtct tggctgacaa attgcagaca ttatgcttta
     5461 attctaaccg gattttcaca tcttgttagt aatctcagca cattttttgg cgtttataat
     5521 gtggttttca actatcacat taaaacgatc agtatgaaga agtttagaaa gctgtggagc
     5581 ttacctgttg cgatcgtctt cttgcttgca agttgtactt ccactgctca tattgaaaaa
     5641 gacgataaca ctgatttcag ccgttataaa acttttgcct ggatagagaa agacggacaa
     5701 ggtaacagta ataaaatcaa gaataacaac ctgactgaac aaaaaatacg gcaggcagta
     5761 aacgctgagc tgataaagac ggcaggttgg agagaagtga agcataagcc ggatgtgatg
     5821 ctcagttatg atgtgctggt agaaagatcc agcagggaaa ttaacagccc ggtttattca
     5881 agacctttta ccaggcttat ttataatcct tatacccgcc ggtatactac tatctattat
     5941 ccatcacagt ttgtagggta cgaaagagat gaaagagcca tacgggaagg aaccgttaca
     6001 ataacaatga tcgattccag aacagataag gcagtatggc agggatggac tacagaggag
     6061 gtgaatagta agaatcttac cacaaaagaa attc
//
LOCUS       DAORLS010000188         6088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7028_length_6088_cov_1.551301, whole genome shotgun sequence.
ACCESSION   DAORLS010000188 DAORLS010000000
VERSION     DAORLS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_6088_cov_1.551301"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    316..373
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <374..>621
                     /locus_tag="PK977_13365"
     CDS             <374..>621
                     /locus_tag="PK977_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRF19156.1"
                     /translation="ITPLLALLAERWHRPHNISLGQWMRFFGIQAFIHLFIDGMNVYG
                     VGWFEPFSHHRVSYNWIYVADPFYSVWLGIAFLMLLIL"
     assembly_gap    622..734
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <735..1183
                     /locus_tag="PK977_13370"
     CDS             <735..1183
                     /locus_tag="PK977_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRF19157.1"
                     /translation="VFKKQNISYTNYFTTPTPLNNWLWFVVASNDSGHYIGYRSLFDS
                     ERKIDFHFVPKNDSLLKQVQDHEDLQKLKRFSKGYYAIQNRNDTLIFNDLRFGQMIGW
                     MNPNAPFAFYYYLQHPSENNLVVQRGRFAGWNKEAFLALLKRIRGN"
     gene            complement(1185..>1894)
                     /locus_tag="PK977_13375"
     CDS             complement(1185..>1894)
                     /locus_tag="PK977_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRF19158.1"
                     /translation="EGEASFALHHICRDMWGDKNLSSIDTSHIETFCDNIPEDEQIMV
                     RVRRNDWVNRKNQIWKTYYDIFSKAEKKITIMCSYFLPGRLFRKKISQAVKRGVHVKV
                     ILAGRSDIMMAKHAERYLYDWLLRNKIEVYEYQETVLHAKLAVYDERKVTIGSYNVNN
                     ISAYASLEMNMDIENHSFAKYVESELEQISTDHCKLITKENYISSTNIFKRIWQRFCY
                     SFINNVLNLFTFYFRQE"
     assembly_gap    1895..1963
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1964..2437)
                     /locus_tag="PK977_13380"
     CDS             complement(<1964..2437)
                     /locus_tag="PK977_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRF19159.1"
                     /translation="MSPISNIYECSQRSIFDIGVFSIYSMSLGRPDSFAAYTIRNRVQ
                     LVYGGKDYFNTLLEIINNAKQCIHLQFYIYENDETGWLITHALIEAAQRKVDVYLQVD
                     GYASQGISKKMSTELKAAGVNLKWFEPLFRSKHFYFGRRLHHKVVVADGLYSLVGG"
     gene            2474..2860
                     /locus_tag="PK977_13385"
     CDS             2474..2860
                     /locus_tag="PK977_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19160.1"
                     /translation="MKTLLFILLSFIAITATVSGILMISQPDGSILQLKPELLAGSPF
                     SNYLVPGVLLACVVGAVNLVAVFLNMMRHRQRYNAAIAGGAVIITWILVQVMMIGTIH
                     WLHILYFSAGILVVLGAYQLKGRWAV"
     gene            2925..3974
                     /locus_tag="PK977_13390"
     CDS             2925..3974
                     /locus_tag="PK977_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19161.1"
                     /translation="MGYTKAFGLANIQQLTKEISRPAGIFWLICTLSFIAVAIFFFFK
                     REWWYFLAVPAIMVSQVLIIQSWPDAKFGTFPNFLILLIIVSAIAQTRFNKMVNFEIK
                     MLLEQPTVRKEMITDDKLNNLPAVVHTWLRKSGIIGKPVIQFVRLKQMGEMRTKQKSK
                     WMRFSARQYFAVQKPQFVWQTTVQVLPMITLSGRDKFKNGEGHLLIKLLSLFTVAKSG
                     NDEKMNSATMIRYLAEISWFPSAALSQFIRWEPVDSFSAKAIMDFQGVRAEGVFHFNP
                     AGDFVRFTADRFMSNGKNAKTEKWLVEVTEYKEFHGYRIPNKNKVTWQLQTGDFNWAN
                     IEITEMDFNFPEIYR"
     gene            4235..4483
                     /locus_tag="PK977_13395"
     CDS             4235..4483
                     /locus_tag="PK977_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRF19162.1"
                     /translation="MKKDMNEELLHEVKAISGFKTHVSIYVVIVGLLWVIWLIKGGLD
                     IHPWPVYPTVAWGIGLFFHYMSAYGIYRKVEKEQDELT"
     gene            4500..4799
                     /locus_tag="PK977_13400"
     CDS             4500..4799
                     /locus_tag="PK977_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF19163.1"
                     /translation="MNFFDGYIGGDKPVVADFFAGWSEPCKLMDPVLMELKEQLGDRA
                     NVVRIDIDKEPSFAEIFKIWSVPTVVIFKQGHEVWRKTGIIPAHEILTNLNYYIR"
     gene            5006..5488
                     /locus_tag="PK977_13405"
     CDS             5006..5488
                     /locus_tag="PK977_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19164.1"
                     /translation="MKKIRSPEKKVGIWIDQEKALIVRIEGEGPPVLEKMKSEVESRI
                     RIRGEGKVSARFGQSFIDDKEKKQHRQHNQREKFFKEIIKEIKNADYLYLMGPGPAKE
                     GLYNALENDQTARIKVILTEPADRMTQQQLIVKIESFFNGKLFIMLKKKLKKDMLEHA
                     "
     gene            complement(5573..>6088)
                     /locus_tag="PK977_13410"
     CDS             complement(5573..>6088)
                     /locus_tag="PK977_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRF19165.1"
                     /translation="LDCGIVVTPLKDSSIKEDILFYEELFVYVSKNNALHNKKYVLPG
                     DIDPTQLWLLEEGHCFRSQILNLCELQKHSGIQFRYETGNIETLKRMVDNSDGITILP
                     ELAVMEFSQPQLKLVKRLKEPSPAREVSLVTHRDHLKTKLIQTLKEEILNIVPKAMQK
                     LNKKKVVDINY"
BASE COUNT         1728 a         1057 c         1235 g         1828 t
ORIGIN      
        1 aacctgcacc ggaagtttga tttgctaatg actgaacaga tgaagaccct ttttgaaatt
       61 caacaggcac aaatggatat gatggaagaa ataaaaacaa ccttaaacta caattcagaa
      121 aacaaataga taatattgtg ctagcaaaaa aaatcattgg attccataac tcatatcgct
      181 ttaggtgcct gcatggggga tgcatttgca ggaaaacaat tgggaaaacg ggctatgttt
      241 ttaggtgcaa tagcacaaag cctgccagat attgattttg ttgcggctgc ctggacaaat
      301 acagcggaga atttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnntaatcac acctttattg gcattactcg cagaaagatg gcatcgtccc
      421 cacaatatct cacttggcca atggatgcgt ttttttggta tacaggcttt tattcattta
      481 tttattgatg ggatgaatgt ttatggggtt ggctggtttg agccttttag tcatcacagg
      541 gtatcttaca actggatata tgtagctgat ccgttttatt ccgtatggtt aggaattgcc
      601 tttttgatgt tgctaatact tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnatgttt ttaaaaagca aaacatatct tatacaaatt attttactac
      781 accgacaccc cttaataact ggctgtggtt tgtagtggcc tctaatgact cggggcatta
      841 tatcggttat cggtcgttat ttgattcaga aagaaagatc gattttcatt ttgtgcctaa
      901 aaatgattct ctactgaaac aggtgcagga tcatgaagac ctgcaaaaat tgaaacggtt
      961 ttcaaaaggt tattatgcta ttcagaaccg gaatgacaca ctcattttta atgatcttcg
     1021 ttttggtcaa atgatcgggt ggatgaatcc aaatgcccct tttgcttttt attattactt
     1081 gcaacaccct tctgaaaata acctggtcgt tcagcgggga cgttttgccg ggtggaataa
     1141 agaggccttt cttgcattac taaaaagaat ccgcggtaac tagtttattc ctgcctgaaa
     1201 tagaaggtga ataaattcag cacattattt atgaaactgt agcagaaacg ctgccagata
     1261 cgtttaaaga tattggtaga agaaatgtaa ttttctttgg taatcaactt gcagtgatcc
     1321 gtgcttattt gttccagttc actttccaca tatttggcaa atgagtggtt ttctatatcc
     1381 atattcatct caaggctggc ataagcactg atattattta cgttgtacga accaatggta
     1441 acttttcttt catcataaac agccagcttg gcatgtagta cggtttcctg gtactcgtaa
     1501 acttctatct tatttcgtaa tagccagtca tataaataac gctccgcatg tttggccatc
     1561 ataatatcag aacggcccgc cagaatcacc ttgacatgta cacctctttt tacagcctgg
     1621 ctgatttttt tccggaatag cctgccaggc agaaaatagc tgcacatgat ggttatcttc
     1681 ttttctgcct tactgaaaat gtcataatag gttttccata tctggttttt ccggtttacc
     1741 cagtcatttc tccttaccct taccatgatc tgttcatctt ccggaatatt gtcacaaaat
     1801 gtttctatat ggctggtatc aatagatgac agatttttat cgccccacat atccctgcag
     1861 atatgatgaa gagcaaatga agcttccccc tcacnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgccc actaatgagt
     1981 aaaggccatc ggctactact actttgtggt gcagtcttct tccaaaataa aaatgtttgc
     2041 tgcggaaaag gggttcaaac cactttaagt tgacgcctgc tgcttttaat tccgttgaca
     2101 ttttttttga tatcccctgt gaagcataac catcaacctg caggtataca tctacttttc
     2161 tttgagcagc ttcaataagt gcatgggtaa tcagccagcc ggtttcgtca ttttcatata
     2221 tataaaactg aagatggata cattgttttg cattgttgat tatttccaaa agggtgttga
     2281 aatagtcctt tccaccgtaa actaattgaa ccctgttacg gatagtataa gcagcaaaac
     2341 tatcaggcct tcccaatgac atagaataaa ttgaaaatac gccaatatca aaaatagatc
     2401 gctgactgca ctcataaatg ttactgatgg ggctcatccg aactggatag taatcataat
     2461 agctttgaat attatgaaaa ccctcttatt catactttta agtttcatag caataacggc
     2521 tactgtttca ggtatattaa tgatcagtca gccagacggg agtattctac aacttaagcc
     2581 ggaattactg gccgggagcc cattcagcaa ttacctggtg ccgggagttt tgttggcatg
     2641 tgttgtaggc gctgtgaacc tggttgctgt tttcctcaat atgatgcgcc acaggcagcg
     2701 ttataatgca gccatagcgg gtggtgctgt tattattacc tggatattgg tgcaggtcat
     2761 gatgattgga actatacatt ggctgcatat cctttatttc agtgctggta tcctcgtagt
     2821 tttaggtgct taccagttaa aaggacgatg ggctgtttaa ttttttcttt gtaatgagaa
     2881 ttttttttac cctattcgtg actgcccacg gcttaataca cctgatgggt tatacaaagg
     2941 ctttcggact tgctaacatc caacaactga caaaggaaat ttcaagacct gcaggtattt
     3001 tttggttgat atgtacactc tcattcattg ctgtggctat ctttttcttt tttaaaaggg
     3061 aatggtggta ctttctggca gttcctgcta tcatggtttc tcaggtattg atcatccagt
     3121 cgtggccgga tgcaaagttt ggcaccttcc caaacttcct gatcctactg ataatagttt
     3181 cagctattgc tcaaaccagg tttaataaaa tggtcaattt tgaaatcaaa atgttactcg
     3241 aacagccaac tgtaagaaag gaaatgatta cagatgataa attgaacaat ctgccagcgg
     3301 ttgtacatac atggttgaga aaatcaggaa taattggaaa accggtaata caatttgtca
     3361 ggttgaaaca aatgggtgag atgaggacca aacagaaaag taagtggatg aggttttctg
     3421 cccggcagta ttttgcagtt caaaagcccc agtttgtatg gcaaactaca gtacaagtat
     3481 tgccgatgat tacgcttagc gggagagata aatttaagaa tggggagggg catttattga
     3541 ttaagttgct ttcgctgttt acagtagcta aatccggtaa tgatgaaaaa atgaattcgg
     3601 caacaatgat ccgctatttg gctgagatta gttggtttcc atctgccgca ttgagtcagt
     3661 tcattcgatg ggagcctgtt gattcgtttt ctgcaaaagc aattatggat tttcagggtg
     3721 tcagggcaga aggagttttc cattttaatc ctgcaggtga ttttgtacgg tttactgctg
     3781 acaggttcat gagtaatgga aagaacgcca aaaccgagaa gtggctggtt gaagtcacag
     3841 aatacaaaga gtttcacgga tatcgcattc caaataaaaa taaagtcacc tggcagcttc
     3901 aaacgggaga ttttaactgg gctaacattg aaataaccga aatggacttc aattttcctg
     3961 aaatatatcg gtaatatcct gtaatcatct ctttgattga cggcaatcat ttttcccggt
     4021 gcaatagcaa ggtaaattcg tactggctca tacataaggt acaatgacat tagttggaca
     4081 aatagtaagc tttaatttga tggatggctg cattaattgt tttggcattg tttttctctc
     4141 atatacgccg ggcggtaggc tggctattaa tgaaattgta cagctttttt attagtgata
     4201 aggaccatgt aacaccagtt taaaagaaat cattatgaaa aaagatatga acgaggaatt
     4261 gttgcatgag gtcaaggcta tttcggggtt caagactcat gtgagtatat acgttgtaat
     4321 agttggattg ctttgggtta tctggctgat taaagggggt ctggatattc atccatggcc
     4381 ggtatatcca actgtagcat ggggcatagg cttgtttttt cattacatgt cagcttacgg
     4441 catttatcgt aaggtagaaa aagagcagga tgaattaact taaataagcc gatgagtata
     4501 tgaatttttt cgatggttat ataggaggtg ataaacccgt ggttgctgat ttttttgcag
     4561 ggtggagtga gccatgcaaa ctgatggacc cggtattgat ggagttaaaa gagcagttgg
     4621 gagatagagc taatgtggtt cggatcgaca tagacaagga gccgtctttt gcagaaatat
     4681 ttaagatatg gtcggtgcca actgtagtca tttttaagca aggacatgaa gtatggcgaa
     4741 aaaccggaat tattccggcc catgagatac tcacaaatct gaattattac atcaggtgaa
     4801 agaagaacca caagtagtat gatggggcaa tacaactgta ttgccccatt ctttataggg
     4861 tcatttcgtt gatcaaaagg tccgccccat cttcatgtgc tggtgatgag aaacatcatt
     4921 ctttcacatg ataaccgtta ttggtatcag gatatttaca ggtgtacatt caatcgtaaa
     4981 gattttatca cctaaatcta ttaccatgaa aaagattaga tctccagaaa agaaagtagg
     5041 tatttggatc gatcaggaga aagctttaat agtccggatc gagggtgaag gcccccctgt
     5101 tctggaaaaa atgaagtctg aagtagagtc aaggatcaga attcgggggg aaggaaaagt
     5161 gtcagccagg tttggtcagt catttattga tgataaagaa aaaaagcagc acaggcagca
     5221 caatcaaaga gaaaagtttt ttaaagagat aatcaaggag ataaaaaatg cggattatct
     5281 ctacctgatg gggcccggtc ctgcaaaaga aggtctttat aatgctttgg aaaatgatca
     5341 aactgccagg ataaaggtga ttttgacaga gccggccgac aggatgacgc aacagcaact
     5401 gatcgtaaaa attgaatcct tctttaacgg caagcttttc atcatgctga agaaaaagct
     5461 aaagaaagac atgctggagc atgcctgagc tacttttaaa ggcgatctta aggtcgcctt
     5521 tttatataaa taagttcttt gaattaagaa tacaggaaac cgctcgataa tatcaataat
     5581 tgatatcgac cactttcttt ttatttagtt tttgcattgc tttgggtact atattcagaa
     5641 tttcttcctt tagcgtttgt ataagctttg ttttcaggtg gtcacgatgt gtcaccaggc
     5701 tcacttccct tgcaggactt ggttctttta accgtttaac gagttttaat tgaggttgcg
     5761 aaaattccat aacagcaagt tctggcagaa tagtaatacc atcgctattg tcgaccatcc
     5821 ttttcaatgt ttcaatattg cctgtttcat acctgaattg aatacctgag tgtttctgga
     5881 gttcacacag gttcagtatt tgtgaccgga agcaatgtcc ttcttccagc aaccagagct
     5941 gggtgggatc aatatcaccc ggcaatacat actttttatt atgcaatgca ttgtttttcg
     6001 atacataaac aaatagctct tcgtagaaaa gtatatcttc tttaatggaa gaatctttaa
     6061 gcggagtgac cacaattccg caatccag
//
LOCUS       DAORLS010000189         6060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7073_length_6060_cov_1.838801, whole genome shotgun sequence.
ACCESSION   DAORLS010000189 DAORLS010000000
VERSION     DAORLS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7073_length_6060_cov_1.838801"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PK977_13415"
     CDS             <1..259
                     /locus_tag="PK977_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19166.1"
                     /translation="NWTFLLELINVSKEENPKISYPSTARTEGIAPSQYGTKGLLSEK
                     FADIEEKYDLTAVGEGFGEKDEEGEDIGFAEEGSAEGEEEA"
     assembly_gap    383..392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <393..1271
                     /gene="miaA"
                     /locus_tag="PK977_13420"
     CDS             <393..1271
                     /gene="miaA"
                     /locus_tag="PK977_13420"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRF19167.1"
                     /translation="GKTAVAIQLAKHFNTAIVSADSRQCYKELNIGVARPSSEELREV
                     NHHFIASHSIHETVTAAGFEQYALQKVQELFQQHDVVIMVGGTGLYIKAFCEGLDSIP
                     EIDPAIRQSIITAYEEKGIGWLQEQLHAKDPAFAAAGEMQTPQRMMRALEVKESTGQS
                     ILSFRKGEKAQRDFNIIKIGLELPKEELHRNIHRRVDQMMQAGLLEEVKQLLPYKQLN
                     ALQTVGYAELFDYFDGKWTLEEAVERIKINTRQYAKRQLTWFRKDKEIQWFAPGDVDA
                     MKKWLDERVSKSMQDY"
     gene            complement(1470..>1966)
                     /locus_tag="PK977_13425"
     CDS             complement(1470..>1966)
                     /locus_tag="PK977_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19168.1"
                     /translation="VFVFLTTFLSVNGQIQNLILSREENGKWLDSLKTLSLDQKLLMI
                     SERLLTDTNVFVRQPYNDRIKVDEKVGNRVYGDGKPTLIIGGYPMIIDNKTATNKILN
                     LTKYLTTECIKEISILSPSDPATTAIYGSPGLSGIIVITLTKKKYLKKFKRLKLQSND
                     YRYT"
     assembly_gap    1967..2030
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2175..>3092)
                     /locus_tag="PK977_13430"
     CDS             complement(2175..>3092)
                     /locus_tag="PK977_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HRF19169.1"
                     /translation="NVVTNKGGITELALAFAGQRNDKFYFGFTLGVPFLRYDRESTFL
                     EADASTDPNNKFDYASITENLQTKGTGVNLKMGIIFKPQEYVRLGLAVHTPTFYRLTD
                     QYSASVTANTENYKGELSQSSSLFTNGEDAAFSYWHFTPYRIMASASYVLREIEDVRK
                     QKGFLTADIEYVNYKSSSYTTDPEGDDSQGTRDYLKSLNKAIDRAYKGAFNFKAGGEL
                     KFTTIMARLGLAYYGNPYKNLAGEKGNRFQLSGGLGYRNKGIFVDLTYVHTMGKDVHY
                     AYRLQNTSFAGAALRQTGGNVLLTLGFKI"
     assembly_gap    3093..3114
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3115..>3374)
                     /locus_tag="PK977_13435"
     CDS             complement(<3115..>3374)
                     /locus_tag="PK977_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19170.1"
                     /translation="TGGGAFAFAINRSGSFSSNVLYRGQNTQNSYSQKFLEEIQNNND
                     RDANSVAQNYPFGTSLAFNTYWIDTIAGGTSGNYQFQTRATV"
     assembly_gap    3375..3384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3385..3734)
                     /locus_tag="PK977_13440"
     CDS             complement(<3385..3734)
                     /locus_tag="PK977_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HRF19171.1"
                     /translation="MKKLFLLLMGSMAATYLPAQEPADALRYAWLTPSGTARQQAVGG
                     AMTSLGGDLSAAYVNPAGLAFYKTGDFVITPGFELWKNKATYYGRTEQDKKNTLSFGT
                     TGIVLGHEENYGKR"
     gene            complement(3807..>4232)
                     /locus_tag="PK977_13445"
     CDS             complement(3807..>4232)
                     /locus_tag="PK977_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19172.1"
                     /translation="FRYNVYTGTCFCNDPWNPYTYWNSYYNPYYSPYYHNYGYVSIKT
                     PSYTKPRTFNLNTYTSNQPGNTKTYGNYNYTPVRSGNTNNTYTPARNSSSNGSVLRNI
                     FNGNNSSSGTKTNTSSGSSNNNSSSGSGSGGGSAPVRRF"
     assembly_gap    4233..4273
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4274..4501)
                     /locus_tag="PK977_13450"
     CDS             complement(<4274..4501)
                     /locus_tag="PK977_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19173.1"
                     /translation="MKLQILLLAVIVAALSSCTTAYKTGQTPDDVYFSKSRPINEYVR
                     VEEENDQYYRYDDEYYDDRYLRMKVRNRNRWN"
     assembly_gap    4636..4829
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4830..>5671
                     /locus_tag="PK977_13455"
     CDS             <4830..>5671
                     /locus_tag="PK977_13455"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRF19174.1"
                     /translation="DALDKMFKDTGHVNAYFPLFIPKNFLSKEAAHVEGFAKECAVVT
                     HYRLKNDPNGGGVVVDPEAKLEEELIVRPTSETIIWNTYKDWIQSYRDLPLLINQWAN
                     VVRWEMRTRLFLRTAEFLWQEGHTAHATKEEAIEETEKMLDVYATFAEEFMAVPVIKG
                     VKTESERFAGAENTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFSNKENQLEYVW
                     ATSWGVSTRLVGALVMAHSDDQGLILPPKIAPLQVVIVPIYKGDEQKAQIGVKVDEII
                     KELK"
     assembly_gap    5672..5727
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5728..>6060
                     /locus_tag="PK977_13460"
     CDS             <5728..>6060
                     /locus_tag="PK977_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF19175.1"
                     /translation="GWKFAEYEMKGVPVRLAIGARDLEKNVVEVARRDTKEKNTVSLD
                     GIAGYVQQLLADIQQNMFNKAKLYRDEHITKADSWDEFEKLLDEKGGFISAHWDGTAE
                     TEEAIKEKT"
BASE COUNT         1661 a         1089 c         1345 g         1568 t
ORIGIN      
        1 gaactggacc ttcctgctgg aactgatcaa tgtctcaaaa gaagaaaatc ccaagatcag
       61 ttacccgtct acagcaagaa cagaaggtat agcgccaagc cagtacggca ccaaagggtt
      121 gctgagcgaa aaatttgcgg atatagaaga gaagtatgac ctgaccgccg ttggcgaagg
      181 tttcggtgaa aaggatgaag aaggagagga tattggtttt gcagaagaag gaagtgcgga
      241 aggagaggag gaagcctgaa aaaagtaaga agtacaaggt acgaagtaag aagcaggagg
      301 tcagaaatcc gggaacggat gtcggaatgc atattgatca tttcttgaaa aaaactttca
      361 caaaaacagt tattgttatt gcnnnnnnnn nnggtaaaac ggctgtggca atacaactgg
      421 caaaacattt taatacagcc atcgtttcgg cagacagccg gcaatgttat aaagaactca
      481 atattggtgt tgcaaggcca tcttcagaag aattgcggga agtaaaccat catttcattg
      541 cttcccattc catacatgaa acagtaacag cagccggttt tgaacaatat gctttacaaa
      601 aggttcagga attgtttcag cagcatgatg tggtgatcat ggtgggagga acaggattat
      661 atataaaagc gttttgcgaa ggactggata gtatccccga aattgatcca gctatacggc
      721 aaagcattat tactgcttac gaagaaaaag gaatcggctg gctgcaggaa caattgcacg
      781 caaaggaccc cgcctttgct gctgccggtg aaatgcaaac cccgcagcgg atgatgcggg
      841 cactggaagt gaaagaaagt acggggcagt ctatcctgtc ttttcgcaaa ggagaaaaag
      901 cacaaagaga tttcaatatc atcaaaattg gcctggaact gcccaaagaa gagctgcacc
      961 gtaatataca cagaagggta gaccaaatga tgcaggccgg cttattagaa gaagtgaaac
     1021 aactgctgcc gtataaacag ttaaatgcac tgcaaacggt aggttatgca gaactatttg
     1081 attacttcga tggaaaatgg acgctggaag aggctgtgga aagaattaaa atcaataccc
     1141 ggcagtatgc caagcggcaa ctgacctggt ttagaaaaga caaagagata cagtggtttg
     1201 cgccgggaga tgtagatgca atgaagaaat ggttggatga aagggtgtct aaatcaatgc
     1261 aggattatta acgagcaggg ctttatgcag gttgggaatt agtattccct ttgccaataa
     1321 ccgcagctaa ttgaaaatat tttgttgaag ttatgtacaa aagcatatag ttattcgtct
     1381 gctggaactg aagctgggat ttgcaattag ctgatgttac aatgtcaggc ccaacttgca
     1441 taaaaaccaa tgttgtatgc cgttcttcat caagtgtacc tataatcatt tgactgtaat
     1501 tttagtcgct taaatttctt caagtacttt ttctttgtca atgttattac aattattcca
     1561 ctaagtcctg gtgaaccata aattgctgtt gttgctgggt cgctggggct tagaatacta
     1621 atttccttga tgcattcagt ggtcaaatat tttgtcaagt tcaatatctt atttgttgct
     1681 gttttgttgt cgataatcat aggatagccg ccaattatta aagtcggttt tccgtcacca
     1741 taaactctgt taccgacttt ctcatcaacc ttaattcggt cattgtaagg ctgtctaaca
     1801 aaaacatttg tgtcagtaag aagccgttca ctaatcatca gtaatttttg gtcaaggctt
     1861 aaagttttca aactgtcaag ccatttacca ttttcttctc ttgataatat taagttttgt
     1921 atctgcccat ttacagataa aaaagttgtg agaaaaacaa aaacaannnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaagtaatt
     2041 tttgcatagc ggtgtgtcag cgtatggctt acaacgtttg tattgtcgaa tatacgaagt
     2101 ggtactttct ttcttaagtc aaaaaaaggc atccgccaac agcagacgcc tttaagcagc
     2161 ggatttgatt gcgactatat tttgaatcct aatgtcagca gtacattgcc accggtttgt
     2221 cttaatgcag cgccggcaaa agaggtgttt tgcaaacgat aggcataatg tacatccttg
     2281 cccatagtat gtacataggt aagatcaaca aaaatgcctt tgttgcgata acccaggccg
     2341 ccgctcagct gaaaacggtt tcctttttca ccagccaggt ttttataagg attgccataa
     2401 taagcaagac ccagcctggc cataatagta gtaaacttca actcaccgcc cgccttaaaa
     2461 ttgaaggccc ctttataagc cctgtcaatg gctttgttca gtgatttaag gtagtccctg
     2521 gttccctggc tgtcatcacc ctccggatca gtagtgtagg aagatgattt gtagtttaca
     2581 tactcaatat ctgcagttaa aaatcctttt tgttttctta catcttcaat ctctcgcagc
     2641 acatacgaag cgctggccat aatgcggtat ggcgtgaagt gccagtagct gaaagccgca
     2701 tcctcaccat tggtgaagag cgaagaactt tgtgataatt cgcctttata attttccgta
     2761 ttagccgtta cggaagcgct gtattggtcg gtcaaccggt agaatgtagg agtatgtact
     2821 gccaaaccga gacgaacata ttcctgcggt ttgaaaataa tacccatttt caggttcaca
     2881 cctgttcctt tggtttgcag gttttcggta atagaggcat agtcaaactt attattggga
     2941 tcagtgcttg catccgcttc gaggaaagtg gattccctgt cgtaacgcag gaagggaact
     3001 cccagcgtaa agccaaagta gaatttatca tttcgctgcc ctgcaaaagc aagggccagt
     3061 tctgttatac cccctttgtt agtaactaca ttnnnnnnnn nnnnnnnnnn nnnnaactgt
     3121 tgcccttgtt tgaaactggt aattaccgct ggtgcccccg gcaatagtat ctatccagta
     3181 cgtattgaac gccaggctgg taccaaaggg atagttttgg gctacactgt tggcatcccg
     3241 gtcattgttg ttttgtattt cttccaggaa tttttgtgaa taggagtttt gggtattttg
     3301 tccgcggtag agcacattgc tgctgaaact acccgatctg tttatggcaa aggcaaaggc
     3361 gcctcccccg gtacnnnnnn nnnntttctt tttccatagt tttcttcatg tcccaataca
     3421 atcccagtgg tgccaaaaga cagtgtattc tttttatcct gctcggtacg gccgtagtaa
     3481 gtggctttgt ttttccatag ctcaaaaccg ggagtgatga caaagtcccc cgttttataa
     3541 aatgcaaggc cggccggatt tacgtaggca gccgaaagat cacctcctaa ggatgtcata
     3601 gcaccaccta ccgcttgttg ccgggcagtg ccgctgggag taagccaggc ataacgaagg
     3661 gcatctgcag gttcttgtgc gggcaaatag gttgctgcca tactgcccat gagcagtaaa
     3721 aatagtttct tcataaaaga agtttagggt agtgagaaag ggttcgtttg atcgaacgga
     3781 atggcatcag ttgacaaggt ttatcgttaa aaccttctca caggtgcact gccgccacca
     3841 cttccgctgc cggaactgct attgttatta ctgcttccgg aagaggtatt ggtcttggtg
     3901 cctgatgatg aattgttgcc gttgaagata ttcctcagca cacttccatt actgctgctg
     3961 tttcttgccg gcgtataggt attgttagta ttgccactcc ttaccggggt atagttataa
     4021 ttaccatagg tcttggtatt tcccggctgg ttactggtat aagtgttcag gttgaaagtt
     4081 cttggtttag tgtagctggg tgtttttata cttacatagc cataattgtg atagtagggg
     4141 ctatagtagg gattgtagta gctgttccag taagtataag gattccaggg gtcattgcag
     4201 aaacaagtgc cggtatatac attataacga aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnngttccag cggttacggt ttcgcacttt catccggaga tagcggtcat
     4321 cataatattc gtcatcgtaa cggtaatact ggtcgttttc ttcttctacc ctaacatact
     4381 catttatcgg gcgggatttg gaaaaataca catcatcagg tgtttgaccg gttttataag
     4441 ccgtagtaca actgctgagt gctgcaacaa taactgccag gagtaaaatt tggagtttca
     4501 tggcaaaagg tttagtcttg ttaagagtga agttactact tttgtgccac caaaaaactg
     4561 agcacaatcg tttgcaattc agcagcaaag attcagccga agcagaaaaa aattgtgttg
     4621 cggcctgtta aattannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gatgcattag acaagatgtt caaagacaca
     4861 gggcatgtga atgcttattt tcccctcttc atccctaaaa attttctaag taaagaggct
     4921 gcccatgtgg agggttttgc caaagaatgt gcagtagtaa cccattaccg cctgaagaat
     4981 gatcccaacg gtggtggagt ggtggttgat cccgaagcaa aactggaaga agaattgatc
     5041 gttcggccaa catctgaaac aattatctgg aatacgtata aggactggat tcagtcttac
     5101 cgggaccttc ctttactcat caaccaatgg gcgaatgtgg tccgctggga aatgcgtacc
     5161 cgtttgtttt tacgcacagc ggaatttttg tggcaggaag gtcatacagc ccatgctacg
     5221 aaagaagaag ccattgaaga aacagaaaaa atgctggatg tatatgccac ttttgccgaa
     5281 gagtttatgg ctgtgccggt gatcaaaggg gtaaaaacag aaagtgaacg atttgccggc
     5341 gctgaaaaca cttactgtat tgaagcctta atgcaggatg gtaaggcatt acaggcaggt
     5401 acttcacact tcctcggtca gaattttgcc aaagcatttg acgttaagtt ttccaataaa
     5461 gaaaaccagt tggaatacgt atgggctacc agttggggag ttagcacaag attagtaggt
     5521 gccttggtga tggcacacag tgatgaccag ggattgatat tgcctccgaa aattgcaccg
     5581 ctccaggttg ttattgttcc gatctataaa ggggatgagc aaaaagcaca gattggggtg
     5641 aaggttgatg aaatcattaa agaattaaaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnggg ctggaaattt gccgagtatg aaatgaaagg
     5761 ggtgccggta aggttggcca ttggtgcaag agacctggag aaaaacgtag tggaagtagc
     5821 ccgtcgtgat acaaaagaaa agaatacggt tagcttggat ggaatagccg gttatgtaca
     5881 gcaattgctg gcagatatcc agcaaaatat gttcaataag gcaaagctgt acagggatga
     5941 acatattacg aaggctgaca gctgggatga atttgaaaaa ttgctcgatg aaaaaggcgg
     6001 ttttatttca gcacattggg atggtactgc cgaaacagag gaggctatta aagaaaaaac
//
LOCUS       DAORLS010000190         6052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7085_length_6052_cov_1.756878, whole genome shotgun sequence.
ACCESSION   DAORLS010000190 DAORLS010000000
VERSION     DAORLS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_6052_cov_1.756878"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK977_13465"
     CDS             complement(<1..415)
                     /locus_tag="PK977_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRF19176.1"
                     /translation="MKKKPTHNFGKFASKKSNAAIKEQFRQEKRKVKKEREDYFEKKK
                     AEARSRSTGAENQQAIRKGHAAAPATHNKPETAGSMPLNKFIAHAGIAARREAAELVK
                     KGLVKVNGELVTEPGHKVSAKDDIRVNGKKIFLAKN"
     gene            complement(536..1228)
                     /locus_tag="PK977_13470"
     CDS             complement(536..1228)
                     /locus_tag="PK977_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19177.1"
                     /translation="MKKIVAAVLAGAVATFSVQAQEIPERKRDGKGIHERHGNMHHRP
                     GGMEFQKLNLTDAQKEQMKAQRENFRKQLDDLKKNENITVKEWKTRMENLRKDQKTKM
                     ESILTSEQKAQLERMKTERKAIHQVDAKARMEKMKIHLGLTEEQAAKIEKNRKEVAEK
                     MKAIRENKSLSDEKKREEMKELMKKQKENMKSVLTEEQLKKMQEGKKPGHRKPGGPGK
                     PHGQKPADKEVI"
     gene            complement(1377..2429)
                     /gene="rlmN"
                     /locus_tag="PK977_13475"
     CDS             complement(1377..2429)
                     /gene="rlmN"
                     /locus_tag="PK977_13475"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRF19178.1"
                     /translation="MMKKTNRNIRHLSLQDIELYFEQMGEKKFRARQVYEWLWQKHAM
                     SFADMTNLSKDLRQKLGESFTLPALTTDATQYSADGTVKSRFKTHDGHLVEGVLIPTT
                     SRYTACVSSQIGCSLSCKFCATGYIDRKRNLDFDEIYDEVVLINQQCERIHGKKLSNI
                     VFMGMGEPLLNYKNVLKSIERITAPDGLAMSPRRITVSTAGVAKGIRQLGEDKVKFKL
                     ALSLHAANDAKRHEIMPINDTNNIKTLIDALNYFYKQTGNEITFEYILFKDFNDSLKD
                     ADELIKIYRQVPADLINIIEYNPIDFARFEKPEEAKVQAFMEYLQKHRVNVRLRRSRG
                     KDIDAACGQLANKDSH"
     gene            2497..3693
                     /locus_tag="PK977_13480"
     CDS             2497..3693
                     /locus_tag="PK977_13480"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRF19179.1"
                     /translation="MNPVYIIDTLRPPVGKYGGILSSVRPDDLLAIVIKALVERNKSL
                     DVNEIEDVIAGAANQAGEDNRNVARMAALLAGLPVTVAGNTVNRLCASGLQAIMDASR
                     QIICGDAELVIAGGVESMTRAPYVLAKNSEAFSRSAEIFDTTMGWRFINKELAVMYHP
                     FTMGETAENVAKQWNISRVEQDEFALASQQRYASAKAAGKWNEELISVTVKKGKETEI
                     ADADEHPRETSLEKLTSLKPAFIKDGTVTAGNSSGINDGAAAMLLASEKSVKKFGLKP
                     MARVISMAVAGVDPSVMGIGPVPAVNKALKRAGLKIDDIGLIELNEAFASQSIACIRD
                     LGLDIGKVNVNGGAIAIGHPLGCSGVRISTTLLYEMKRRGSRFGLATMCVGVGQGAAI
                     IYENML"
     gene            3746..4129
                     /locus_tag="PK977_13485"
     CDS             3746..4129
                     /locus_tag="PK977_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19180.1"
                     /translation="MKKFLLSLLLIIGSVSLNAQTDSLEQYTGKYKFPDGSPVTEIGI
                     VVENGILMATSAMGNSEFRRTDTKDVFEVVAYAGTATFRRNDQNKVVGVLIQVQDITM
                     EGTKSDLFKEFSLISAKASTIRVCN"
     gene            4349..>5489
                     /locus_tag="PK977_13490"
     CDS             4349..>5489
                     /locus_tag="PK977_13490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF19181.1"
                     /translation="MKKVYLLLLGMLCITLQLLAQRTVTGKVTDDKGNPLPNVTVQVK
                     GTTAGTSSGNDGSYSIVVPANGRVLVFSSVDMETTEVTIGNQSVINTSLKTAEKALQE
                     VVVTGYQTVRKKDVTAAISKISAADIDNLPMPNFAQAMQGRAAGVIVAAANGVPGGSL
                     SVIIRGVGSINAGTTPLYVVDGIQLNTGVGSINTQNNPLNFLNPDDIESIEILKDAAA
                     ASIYGARAGNGVILVTTKKGKSGKPKFTFNSYIGRSTPLGTVDVLSTQNWLRLRIEAL
                     VNSGVAPATARSTALSNLGLPASTSDEKIDSLPTYDWQDAAFGTGTISNVEMSMNGGS
                     QNINYYLSASYSKQTAFIAPTNFQRGALLSKTSFKINDKVTIDNSL"
     assembly_gap    5490..5521
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5522..>6052
                     /locus_tag="PK977_13495"
     CDS             <5522..>6052
                     /locus_tag="PK977_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19182.1"
                     /translation="QNAPYSLGNTGFGNPAYASAMILPINPIYNPDGTYFGLPGSGQN
                     MVGTFNHNVMAVGDYVKYFTRTNQFIGSASVTYKPIPSLTLKSLIGIDYRLTQDHRYQ
                     DPRVNDGFAVNGRLSDQSDWNTNLISTTTANFNKTFKDVHNVTALAGVEYRRDQNQWI
                     QADAQGFPSYLLQYLSA"
BASE COUNT         1599 a         1193 c         1313 g         1915 t
ORIGIN      
        1 ggttttttgc cagaaagatt tttttcccat taaccctgat atcatctttt gcagaaactt
       61 tatggccggg ttctgtcact aattcaccat tgacctttac aagtcctttt ttaaccaatt
      121 cggcagcttc acgccgggct gctataccgg catgggcaat aaatttattc agtggcatac
      181 tgcctgctgt ttcgggttta ttatgcgtag ctggtgcggc agcatggcct ttgcgtatcg
      241 cctgttgatt ttcagcacct gtacttcttg acctggcttc agctttcttc ttttcaaagt
      301 aatcttcccg ttcctttttt acttttcttt tttcctgcct gaactgttct ttaatggcgg
      361 cattactttt tttgcttgca aacttgccaa aattgtgagt gggtttcttt ttcatgatga
      421 aaattttctg ttttacaaca gtagtagggt gtaaagatag tctttagccg gtcaaccatg
      481 gaaaaagaaa agccccggaa tacggggctt taatgtcttg tacgggtatc cccgtttata
      541 tcacttcttt atcagcaggt ttttgtccgt gaggtttgcc tggaccacct ggttttctgt
      601 gaccaggttt tttaccttcc tgcatttttt taagttgttc ttctgtaaga acagatttca
      661 tattctcttt ctgctttttc atcagctctt tcatttcttc ccttttcttt tcatcactaa
      721 gagatttatt ttcacggatc gctttcattt tttcagccac ctcctttcgg ttcttttcaa
      781 ttttagctgc ttgttcttca gtaaggccca ggtgtatttt catcttttcc atcctggctt
      841 tggcgtctac ctgatgaatt gcttttcgtt cggtcttcat tctttccaac tgagcttttt
      901 gctcactggt tagaatgctt tccatttttg ttttttgatc cttgcgaagg ttttccatcc
      961 tggttttcca ttcttttaca gtgatgttct catttttttt cagatcatcc agttgcttac
     1021 ggaaattttc ccgttgtgct ttcatctgtt ctttctgagc atcggtgaga ttcaattttt
     1081 ggaattccat tccaccggga cggtgatgca tgtttccgtg gcgttcatga attcctttcc
     1141 cgtcacgttt acgttccggg atttcctgtg cttgtacaga gaaggtggct acagcacctg
     1201 ctaaaactgc tgctactatt tttttcatgg ttatcatttt tagtttggac gccgtcttgt
     1261 tcttaaaact ttcggcacaa cttgttataa aatttgtgga cgaaataaag accttaacca
     1321 atatagcagg tttaaagaga actatgaatt tcttcgtaag tgggtaaaaa cagggattaa
     1381 tggctgtcct tattagccaa ctggccacag gcggcatcaa tatcctttcc ccggctacgg
     1441 cgcaaacgta cattgacacg gtgtttttgc aggtactcca taaaggcctg tactttggct
     1501 tcttcgggtt tttcaaaccg ggcaaaatcg attggattgt attcaattat attaattaga
     1561 tcggcgggaa cctgcctgta tatcttaatc agttcgtctg catctttcaa tgaatcgttg
     1621 aaatctttaa aaaggatata ctcaaaagtg atttcgttac cagtctgttt atagaaatag
     1681 ttgagggcat caatgagtgt tttaatgtta ttggtgtcat taataggcat tatctcatgc
     1741 cgctttgcat cgttggctgc atgcagggat aatgccagtt taaattttac cttgtcttca
     1801 cccaattgcc ttattccttt ggccacaccg gcggtggaga ctgtaatacg gcgtgggctc
     1861 atagccagcc cgtccggagc agtgatgcgt tcaatggatt taagtacatt tttataatta
     1921 agcaggggtt cacccatacc catgaaaacg atattactga gtttttttcc atgaattctt
     1981 tcacattgct ggttgatgag taccacttca tcgtatatct catcaaaatc aaggtttctt
     2041 ttgcggtcta tgtatcctgt ggcacaaaat ttacaactga gtgagcagcc gatttgtgaa
     2101 gaaacacagg ctgtataacg ggatgtagtg ggtataagca caccttccac caggtgtccg
     2161 tcatgtgtct tgaaacggga tttaactgtt ccgtcagctg agtattgcgt agcatcggta
     2221 gttaatgcag gaagtgtaaa ggattcccct agtttttgtc gcagatcctt gctaaggttg
     2281 gtcatatcgg caaagctcat agcatgtttt tgccaaagcc actcataaac ctgcctggct
     2341 ctgaactttt tctcgcccat ttgctcaaaa tacagttcaa tgtcctgtag ggataaatgc
     2401 cgtatgttcc tgttcgtttt cttcatcatc tgcaaagata gtctgcaatc aaggcattca
     2461 tcggatttca cagatatttg ctttcaaata ttttaaatga atccagttta tataatagat
     2521 accttaagac ccccggttgg caagtatgga ggtatactca gttccgtcag gcctgatgat
     2581 ttactggcca ttgttatcaa agccctggtt gaaagaaata aatctttaga tgtaaatgaa
     2641 atagaagatg taatcgccgg ggccgctaac caggccggcg aggacaaccg gaatgtagca
     2701 agaatggcgg cattgttggc agggttgccg gtaacggtgg ctggaaatac ggtcaaccgg
     2761 ctttgtgctt cgggattaca ggctattatg gatgcctcga ggcagatcat atgcggagat
     2821 gcagaactcg ttattgccgg cggagttgag agtatgacaa gggctcccta tgtattggca
     2881 aaaaacagtg aggccttcag ccgttcggca gaaatatttg atacaacgat gggatggcgg
     2941 ttcattaata aagaactggc ggtgatgtac catccattta caatggggga gactgcggag
     3001 aatgttgcta aacaatggaa cataagccgg gttgaacaag atgagtttgc attagcttct
     3061 caacagcgtt atgcttcagc aaaagcagcg ggcaagtgga atgaggaact aatttccgta
     3121 acagtaaaga aaggaaaaga aacagaaata gcagatgctg atgagcatcc acgggaaact
     3181 tctttggaaa aattgacttc gttgaagccg gcatttatca aggacggaac agttacagct
     3241 ggtaattctt cggggattaa tgatggagcg gcagctatgt tgctggccag cgaaaaatca
     3301 gtcaaaaaat ttggattaaa gcccatggca agggttattt cgatggctgt ggcaggtgta
     3361 gatccatcag ttatgggtat cggcccggtc cctgctgtaa ataaggcttt gaaaagggca
     3421 ggtttaaaaa ttgatgatat tggtcttatt gagttaaacg aggcatttgc atcgcaatcc
     3481 attgcttgta taagggatct tggccttgat ataggaaaag taaatgtaaa cggcggagca
     3541 atcgccatag gtcatcccct gggttgcagc ggggttcgca tttcaactac tttattatac
     3601 gagatgaaaa gaaggggttc ccggtttggc ttagccacta tgtgtgttgg ggtaggtcag
     3661 ggagcagcaa ttatttatga gaatatgtta tgaattgata acttttctta tttaaattcg
     3721 ttaagtcacc tcaaatacga aatgcatgaa aaaattcctt ttatcacttt tgctgatcat
     3781 aggcagtgta tctctcaatg cccaaactga ttcattggag cagtatactg gcaaatacaa
     3841 atttcccgat ggaagcccgg ttacggaaat tggtattgtc gttgaaaacg ggatattaat
     3901 ggccacatct gctatgggta attcagaatt caggagaact gatacaaaag atgtatttga
     3961 agttgtagct tatgccggta cggcaacttt tcgaagaaac gatcagaata aagtagtggg
     4021 ggttttgata caggtacagg atattacgat ggaaggcaca aagtcagatt tgttcaaaga
     4081 attctcttta atttccgcta aagcctcgac aatccgggtc tgtaattaac ctctttatgt
     4141 accaaataca tggttatcag cctaaaatat ttattttttt taattattat ttctttcatt
     4201 ctctaaattc gtgcagcaaa ttttgaacat agattgtcaa aatttgtgaa ggctctgaaa
     4261 tgcaactgct gtaatacttc actgtattca agcaagattc atttcttatt tactgtctgc
     4321 ttatttttta acaactaaaa ctaactgcat gaagaaagtt tatctgcttc ttctgggcat
     4381 gctgtgtatt accctgcagc ttcttgccca acgaacagtc accggtaaag tgactgatga
     4441 caaggggaat ccacttccca atgttaccgt tcaggtaaag ggaactactg ccggaacatc
     4501 aagcggtaat gatggcagct attccatcgt tgttcctgct aatgggcggg ttcttgtttt
     4561 ttcatctgtc gatatggaga cgactgaagt gacaatcggc aatcaatcgg tcatcaacac
     4621 ttcattaaaa acagctgaaa aggctttaca ggaagtggta gtaaccggtt atcaaacggt
     4681 aaggaagaaa gatgttacgg ccgctatttc taagatcagt gcagctgata ttgacaatct
     4741 tccgatgccc aactttgccc aggcaatgca aggccgtgct gccggtgtga ttgtagccgc
     4801 agcgaatggt gtgccgggtg gttcattaag tgtaattatt cgtggtgttg gttctattaa
     4861 tgccggtact actcctttgt atgttgttga cggaattcag ttaaatactg gtgttggttc
     4921 aatcaacaca cagaataatc cgctgaactt tttaaaccca gatgatattg agtcaataga
     4981 aatattgaaa gatgcggcgg ctgcttctat ctatggtgcc cgggctggta atggtgtaat
     5041 attggttact actaaaaagg ggaaatcagg taagcctaag tttactttca attcgtatat
     5101 aggcagatct acacctctgg gaacagttga tgtattgtca acgcaaaact ggttaagatt
     5161 gagaattgaa gccttggtaa actccggtgt agctcctgca acagccagga gtactgcatt
     5221 atccaatctt ggtttgccag ccagtacttc agatgagaaa attgacagtc ttcccactta
     5281 cgactggcaa gatgcagcat ttggcacagg caccatttca aacgtggaaa tgtcaatgaa
     5341 cggaggctct caaaatatta attattattt gtcagcctcc tattcaaagc aaacagcttt
     5401 cattgcgcca acaaactttc aaagaggtgc tttattgtca aagacaagtt ttaagattaa
     5461 tgataaggtt acaatcgata attctttaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 ncagaatgcg ccatatagtt tgggtaacac cggatttggc aaccctgcct atgcatctgc
     5581 catgatctta ccgataaatc cgatttataa cccggacgga acatattttg gattgccagg
     5641 ttctggtcaa aatatggttg gtacgtttaa ccataatgtg atggcagtag gtgattatgt
     5701 gaaatacttc acccgtacaa accagtttat cggaagcgct tctgttactt ataaacctat
     5761 acccagcctt accttaaagt cattgattgg tattgattac aggctgacac aagaccaccg
     5821 ctaccaggat ccaagggtca atgatggatt tgctgtaaat ggacgtttgt cagatcagag
     5881 cgattggaat acaaacctga tcagtactac aacagctaac tttaataaga ctttcaaaga
     5941 tgttcacaac gtaactgcac tggcaggtgt tgaataccgc cgtgatcaga accagtggat
     6001 tcaggctgat gctcagggct tcccatccta cctattacaa tacctgagtg ct
//
LOCUS       DAORLS010000191         6037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7113_length_6037_cov_1.876797, whole genome shotgun sequence.
ACCESSION   DAORLS010000191 DAORLS010000000
VERSION     DAORLS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7113_length_6037_cov_1.876797"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PK977_13500"
     CDS             <1..844
                     /locus_tag="PK977_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF19183.1"
                     /translation="DSEIVNAICDHPGIEAVSFVGSTKVAKIVYQRATQHYKRALALG
                     GAKNHLMVLPDAKPEMTAQNVAASMSGCAGQRCMAASAMVAVGDVDHIISKICDEARK
                     IVPGENLGAVISKESKERIEGYITEAEKQGAKILVDGRNAKVKGKENGTYVGPTVIDY
                     VKPDMAVAKEEIFGPVISIMRTKTVDEALAIENANPYGNAASVFTQNGGMSRYIIDRA
                     SAGMIGVNVGVPVPREPFSFGGWNESKFGVGDITGKSSIEFWTKLKKSTTKWNPEAGV
                     NWMS"
     gene            889..2232
                     /locus_tag="PK977_13505"
     CDS             889..2232
                     /locus_tag="PK977_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF19184.1"
                     /translation="MIKTVSEAEEILQENLDYTLFSWSKQKGIAPIAVKHAEGVYLYD
                     YDGKRYIDFSSGLMNVNIGHGNQRVTDAVVKQMQEVAYVTPSCVTKVRGELGKKLAEI
                     CPGDLNKAFFTLCGATSIENGIKLARLYTGRHKILTRYQSYHGASYGAMSASGDPRRL
                     PMDTQQAPNFVHFDLPYLYRWEYGEENLLKESVASLERVIAYEGPANIAAILLEGESG
                     SSGCLKYPVGYLKAIREICNRHGILLIIDEVMSGFGRTGKWFGFENHGIVPDMICMAK
                     GLTAGYLPFGCLMVSDKIAAKYDDTVLSLGLTYSAHPVSCAAALETIKIYEDEDLIEN
                     CVTMGQYVNEQVELLKQKHPSIGDFRNTGLLGCIELVKNRKTKEPMAPFNAKPDEMTV
                     MNKVAAKIKDLGMYTFVRWSYIFIAPPLCVTKEQIDEGLKIISEAIAIADQHYVE"
     gene            2302..>3111
                     /locus_tag="PK977_13510"
     CDS             2302..>3111
                     /locus_tag="PK977_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRF19185.1"
                     /translation="MNGNQSIYDNSLINEDLAPVSPEKRTWGTWNYAALWISMSLCIP
                     TYMLASSLIGGGMNWWQAILTIFLGNTIVLIPMILNGHAGAKYGIPFPVFARASFGTK
                     GANIPAILRAIVACGWFGIQTWIGGDALYHILKAWNPSLAEVDTQSLFPQAVPMICFL
                     VFWSLNMYIVYLGVESIRKLLVFKAIFLPVAALALLAWAITAAKGLGPILEQPSKLHG
                     NDFWKYFFPALTGMVGFWATLSLNIPDFTRYAKSQKAQISGQAIGLPPSMTL"
     assembly_gap    3112..3170
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3171..3754
                     /locus_tag="PK977_13515"
     CDS             <3171..3754
                     /locus_tag="PK977_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRF19186.1"
                     /translation="DPVQLAGKFENKLMVSFAMLAVIISTLATNIAANIVSPANDFAN
                     LAPKKINFKTGGYITGIIGILIFPWKLVADPSGYIFTWLVGYSSLLGPVGGIMIADYY
                     FIRKQELVLDELYRSSGRYSFSKGFNTNAIIALLTGILPNIPGFLVTIKVIAADTVPY
                     WISNLYNYAWFVGFFISGFVYWLLMKRKNYVEF"
     gene            3829..>4759
                     /locus_tag="PK977_13520"
     CDS             3829..>4759
                     /locus_tag="PK977_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19187.1"
                     /translation="MSILIKNGRIVTATDDYVADIFIEGEQVKAIGKNLAVKADKEID
                     ASGKLVFPGGIDPHVHLDMPFMGTFSSDNYETGTRAALFGGTTMVIDFILQKQGNSLQ
                     AALDEWRGRSDGNCLGDYSFHMAVTDYNENSKKEIQGLIEKEGITSFKTFMAYKGALM
                     IDDRQMVGLMQEVKKHGGLINVHATNGDMIDYLVAKHRAEGKLSPLYHYLSQPEVTEA
                     EASGRFADLANYTGCPGYIVHLTCEGALNAVRNATRRNQKVFVETCIQYLIIDASLYE
                     REDGAKWVMSPPLRQKKDQETLWAGINQGLVQVV"
     assembly_gap    4760..4867
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4868..5197
                     /locus_tag="PK977_13525"
     CDS             <4868..5197
                     /locus_tag="PK977_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19188.1"
                     /translation="VNKGRITLNKYVEVACTNPAKIFGMFPRKGTIAVGSDADIVIFD
                     PTEKHTLSAKTHHMNVDYSGYEGWEVTGKVKTVLLRGKVVIENNQCLVDKGYGKFIKR
                     SQVSQVI"
     gene            5282..>6037
                     /locus_tag="PK977_13530"
     CDS             5282..>6037
                     /locus_tag="PK977_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRF19189.1"
                     /translation="MPRKIKSGLIQMSLPKTEGEGTIEEIKEAMVQKHIPYIEEAGKK
                     GVQILCLQEIFNTPYFCPGQDKAWYESAETIPGPTVERMQEYAKKYSMVMIVPMYEKE
                     QAGVLYNTAAVIDADGTYLGKYRKNHIPHTSGFWEKFFFKPGNLGYPVFQTKYAKVGV
                     YICYDRHFPDGARILGLNGAEIVYNPSATVAGLSQYLWKLEQPAHAAANGYFMGCINR
                     VGTEKPWNLGKFYGSSYFVDPRGQIFAQASEDND"
BASE COUNT         1787 a         1209 c         1351 g         1523 t
ORIGIN      
        1 tgacagcgaa attgtcaatg ccatctgcga tcacccgggt attgaagcag tttcttttgt
       61 tggctctacc aaagtggcaa agattgttta ccaaagggca actcagcatt ataaaagagc
      121 tttggcactc ggtggcgcca aaaatcattt gatggttttg ccggatgcaa aaccggaaat
      181 gactgcacag aatgttgccg caagtatgag tggctgcgcc ggtcagcgtt gcatggcagc
      241 gtcagccatg gttgcagtgg gtgatgtgga tcatattatc agcaagattt gcgatgaagc
      301 aagaaagatt gtacccggag aaaacctcgg agctgtaatc agcaaagaat caaaagaaag
      361 aattgaaggt tatatcactg aagcggaaaa gcaaggtgca aaaattttag tagatggccg
      421 caacgccaaa gtaaaaggaa aagagaacgg cacttatgtg ggaccgacag taatagatta
      481 tgtaaaacca gacatggcag ttgccaaaga agaaattttt ggccctgtca tttctataat
      541 gagaacaaaa acagttgacg aagcattggc tattgaaaat gccaatcctt atggtaatgc
      601 agcttccgta tttacacaga atggcggcat gtcccgctac ataatcgacc gtgccagcgc
      661 cggcatgatc ggcgtaaatg taggcgtgcc ggttccaaga gaacccttct cctttggcgg
      721 ctggaatgaa agtaagtttg gggtaggcga tattaccggt aaaagttcga ttgagttctg
      781 gactaaactt aagaagagta caacgaagtg gaaccctgag gcgggagtga actggatgag
      841 ctaaacctat tttgaatgta ttcattcaaa atttaaaatt caaaataaat gattaagaca
      901 gtttctgaag ccgaagaaat tctccaggaa aatttagact acacactctt ttcctggagc
      961 aaacaaaaag gcattgcccc aatcgccgtt aaacatgccg aaggcgttta tctctacgac
     1021 tacgatggca aacgctatat tgatttctcc tcgggactga tgaatgtaaa tattggtcat
     1081 ggcaatcaaa gagtaactga tgcagtggta aaacaaatgc aggaggtcgc ttatgtaacg
     1141 ccgagttgcg taacaaaagt tcgtggtgaa ttaggaaaaa aactcgctga gatttgtccc
     1201 ggcgatctga acaaagcttt ctttacactt tgtggtgcga cttctattga aaacggaatt
     1261 aaacttgcaa gattatatac cggtcgtcat aaaatattaa cacggtacca gtcctatcac
     1321 ggcgcctcct acggagctat gagtgcaagc ggagatccaa ggcgtttacc catggataca
     1381 caacaggccc ccaactttgt tcattttgat ttgccttatc tctaccgctg ggaatatgga
     1441 gaagaaaatc tattgaaaga gtctgttgca tcgttggaaa gagtgatcgc ttacgaaggc
     1501 cctgccaata tcgcagcaat tttactggaa ggtgaaagcg gttcatccgg ttgtttgaaa
     1561 tacccggttg gttatttaaa agcaataaga gaaatctgca accggcatgg gatcttatta
     1621 ataatagatg aagtaatgag tggttttggc cgtaccggca aatggtttgg ttttgaaaac
     1681 catggtatcg ttccggatat gatctgcatg gcaaaaggat tgacagccgg ttacctcccc
     1741 ttcggttgtt taatggtaag tgataagatc gctgccaaat atgatgatac tgttctatcc
     1801 cttggcctca cctactctgc ccacccggtc agttgtgctg ctgcactgga aacaattaaa
     1861 atctatgagg atgaagacct gattgaaaac tgtgtgacaa tgggacaata tgtaaatgaa
     1921 caggtggaat tattaaagca aaaacatccc agcatcggcg atttcaggaa tacaggctta
     1981 ttaggttgta ttgaactggt gaagaacagg aagacaaaag aaccaatggc tccttttaat
     2041 gccaagcctg atgaaatgac cgtgatgaat aaagtggcag ctaagatcaa agacctggga
     2101 atgtacacat tcgttagatg gagttatatt ttcattgccc cgccgctttg tgttaccaaa
     2161 gaacagatcg atgaggggtt gaagatcatt agtgaggcaa ttgcaatagc agatcagcat
     2221 tatgttgaat gataaatttt gaatgctaaa tgaatggctg tttttcattc agcatttaac
     2281 attcaacatt caaaataaat aatgaacgga aatcaaagca tatacgacaa ctccctaatc
     2341 aacgaagacc tcgcccctgt ttcaccggaa aaacgaacct ggggcacctg gaactatgcg
     2401 gcattatgga tcagcatgag cctttgtatc ccgacttata tgcttgccag ttcgctgatt
     2461 ggtggcggca tgaattggtg gcaggccata ctcactattt ttctcggcaa tacaattgta
     2521 ttaataccca tgatcctgaa cggtcatgcc ggcgcaaaat atggcatccc cttccctgtt
     2581 tttgcaaggg ccagctttgg cacaaaaggc gccaatatac cggccatact cagggctatc
     2641 gtcgcctgcg gctggtttgg catacaaaca tggatcggcg gagatgcatt gtaccatatt
     2701 cttaaagcat ggaatccatc cttagcagaa gtggatacac aatcattatt tccgcaggca
     2761 gtaccaatga tctgtttcct tgtgttttgg tcgctgaaca tgtacatcgt ttatcttggc
     2821 gtcgaaagca tccggaaatt attagttttc aaagcgatct ttttgcctgt tgcagcattg
     2881 gctttattgg catgggccat tacggccgca aaaggattgg ggccgatatt ggagcagcct
     2941 tcaaaattac acggtaacga tttttggaag tactttttcc ctgcactcac cggcatggtt
     3001 ggcttttggg caacactatc tttgaacatt cccgatttta cgagatacgc taaatctcaa
     3061 aaagcacaga tcagcgggca ggctattggc cttccgccat caatgacatt gnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggatcctgt
     3181 tcagctcgca ggtaaatttg aaaacaaact catggttagt ttcgcaatgc tggccgtgat
     3241 catttctaca ctggccacca atattgctgc gaatatcgta agcccggcca atgactttgc
     3301 aaacctggct cccaaaaaga tcaatttcaa aaccggtggt tacatcacgg gcatcatcgg
     3361 catcctcatc tttccctgga aattagtagc cgatcccagt ggttatattt ttacctggct
     3421 ggtgggctat tcaagtttac tggggcccgt aggcggcatc atgatcgccg attattattt
     3481 tatacgcaaa caggaactgg tgcttgacga gttgtaccgg tcatccggcc gatatagttt
     3541 cagcaaagga tttaatacaa atgcaattat cgcattgctg accggtattc tgcccaacat
     3601 tcctggcttc ctggtaacga tcaaagtaat agcggcagat actgttcctt actggataag
     3661 caacctttat aactacgcat ggttcgttgg gttctttatc agcggttttg tatattggtt
     3721 attgatgaag agaaaaaatt atgttgaatt ttaaatgctg aattttgaat gacgaagcat
     3781 tcgctttcat tcaaaattta aaattcaaca ttcaaaataa taatacaaat gagtatatta
     3841 attaaaaacg gaagaattgt tactgccact gatgattatg ttgccgacat cttcattgaa
     3901 ggcgagcagg taaaagctat tggtaaaaat ctggcagtga aagccgataa agaaattgac
     3961 gcctccggta aactcgtttt ccccggtggt atagacccgc atgtacacct cgatatgccg
     4021 ttcatgggaa ctttctcttc cgacaattac gaaacaggaa ccagggcagc cttgtttggc
     4081 ggcaccacca tggtgattga ttttatcctg cagaaacaag gtaattcatt acaggctgct
     4141 ttggacgaat ggagaggccg cagcgatggc aactgtctcg gtgattacag tttccacatg
     4201 gcggtgaccg actacaacga aaacagcaaa aaagaaatac agggcctcat cgaaaaagaa
     4261 ggcatcactt ctttcaaaac attcatggcc tataagggag cattgatgat tgatgaccgc
     4321 cagatggtgg gactgatgca ggaagtaaaa aaacacggtg gcctgatcaa tgtacatgcc
     4381 accaacggcg atatgatcga ttacctcgta gccaaacacc gggccgaagg aaagctgtca
     4441 ccgctgtatc attatctttc acaacctgaa gtaaccgaag cagaagccag cggacgtttt
     4501 gctgacctcg ccaactatac cggttgtccc ggttatatcg ttcacctcac ttgtgaaggc
     4561 gcattaaatg cagttcgtaa tgccacaaga agaaaccaga aagtatttgt ggaaacctgt
     4621 attcaatatc tcatcattga tgcttcttta tacgaaagag aagatggcgc caaatgggtg
     4681 atgagccctc ccctgcggca aaagaaagac caggaaacat tatgggcggg aattaatcaa
     4741 ggcttggtac aggtagtggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnngtc aataaaggca ggatcacatt gaacaaatat gtggaagtgg cctgcaccaa
     4921 cccggcaaag attttcggca tgttcccccg caaaggaacc atcgcagtag gcagtgatgc
     4981 cgacatcgtc attttcgatc caactgaaaa acataccctt tccgccaaaa cacatcacat
     5041 gaatgtagac tacagtggtt acgaaggatg ggaagtaacg ggtaaagtaa aaacagtgtt
     5101 actgagaggt aaagtagtta tcgaaaataa tcagtgcctc gtggataaag gatacggtaa
     5161 attcattaaa cgttctcaag tatcgcaagt gatttaaatt attttaaatt ttgaatttta
     5221 actgttgaat cagttgcctt tcacttaaaa ttcaaaattc aacattcaaa ataacaatat
     5281 aatgccaagg aaaattaaat ccggactaat ccagatgagc cttcccaaaa ccgaaggcga
     5341 aggaaccatt gaagaaataa aagaagcgat ggtgcaaaag cacatccctt atattgaaga
     5401 agcaggaaaa aaaggtgttc agatactttg cctgcaggag atctttaaca ctccttactt
     5461 ctgccccgga caggataagg cctggtatga aagtgctgaa acgattcccg ggccaaccgt
     5521 agagcggatg caggaatacg ccaaaaaata cagcatggtg atgatcgtcc cgatgtacga
     5581 gaaggaacag gccggtgtgc tgtacaatac tgctgccgtg attgatgcgg acggcactta
     5641 tttaggcaag taccggaaga atcatattcc gcataccagc ggtttctggg aaaaattttt
     5701 cttcaaaccc ggcaacctcg gttatcctgt ttttcaaaca aaatatgcta aggtgggtgt
     5761 ttacatctgt tacgaccgtc actttcccga tggggcaaga atattaggtt taaatggtgc
     5821 agagatcgtt tacaatcctt ccgcaacagt agcaggtctg tcgcaatact tatggaaact
     5881 ggaacaaccg gcccatgcag ccgccaatgg ttatttcatg ggttgcatca accgggtggg
     5941 aacggaaaaa ccctggaatc tcggtaagtt ctatggcagc agttatttcg ttgatccaag
     6001 aggacaaata tttgcacaag ccagtgaaga caatgat
//
LOCUS       DAORLS010000192         6007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7164_length_6007_cov_1.820901, whole genome shotgun sequence.
ACCESSION   DAORLS010000192 DAORLS010000000
VERSION     DAORLS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_6007_cov_1.820901"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..536)
                     /locus_tag="PK977_13535"
     CDS             complement(54..536)
                     /locus_tag="PK977_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRF19190.1"
                     /translation="MVQLDNTDINILQLLQHDARLTNKEIADKLGKSVTPVYERIKWL
                     EQEGYIQRYVAVLDKNKIDKNLVAFTNVQLKEHSHPMLKAFERDIVKLSEVMECHHMT
                     GIYDYLLKIVVKDMNEYQDFIVNKLAKLPNIGTVQSGFEMTEVKYETAYTITLPEKKK
                     "
     assembly_gap    834..850
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <851..1985
                     /locus_tag="PK977_13540"
     CDS             <851..1985
                     /locus_tag="PK977_13540"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF19191.1"
                     /translation="HRKKKKHEKIGLIYSRINNPDMEILEDRLTLWDGAEACAVFESG
                     MSAISTTMLEFLKPGDLLLYSNPIYGGTHHFIHHVLTKFGIHIMGFRAGETMDEIEMR
                     LKGSGLSDKLGMVYLETPANPTNDLIDIEMCVALARKYSVADKNVIVSVDNTYIGPIW
                     QHPLKNGADLVLYSATKYIGGHSDVIAGAVLGNKELIGRVKVLRTFLGNMISPYTGWL
                     LLRSLETLKVRMDKQAENAQKVVDWLKQHPLVEKVYYLGCLEHSNPSQHAIYRKQCLG
                     NGAMLSFDITGGEKEAFTFLNNLKLIKLAVSLGGTESLAEHPATMTHVDIPDEERAHY
                     NITSKLIRLSIGVENPQDLIWDIDQALEKVAQLDHIYNKEVAF"
     assembly_gap    2183..2222
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2223..>3355
                     /locus_tag="PK977_13545"
     CDS             <2223..>3355
                     /locus_tag="PK977_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF19192.1"
                     /translation="LLTHAEGTGPKRSRRPVYMDFMPPCNSACPAGENIQAWMAHAQS
                     GNYFEAFQTILEDNPFPAIMGRVCVKPCETGCNRNHIESTVNIHAVERFIGDEAIRQK
                     WPVQYMYEETGKRILVIGAGPGGLSAAYHLARMGHSVEIFDAGKHPGGLLWTGVPEYR
                     LPKEILDAEVERIVKMGVKIRLNYKVKDVLTEKHAGNFDAVYLSIGAQLIHDEGFVHD
                     SSVYITDAFSFFSESKLNNSPFVQKKVVVYGGGKLALYLSRILKRYGSEVSVYYPGDK
                     KMMPAYDYETEDALAEGVDVQLYRSISKIEKKKITLEKMKVEKNKAVGTGEFETVDAD
                     VLINAYRQETDSGFLRSVSAIKVNEDGTVVIDAQRMTGHEGIFA"
     assembly_gap    3356..3365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3366..>3692
                     /locus_tag="PK977_13550"
     CDS             <3366..>3692
                     /locus_tag="PK977_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217545.1"
                     /note="unknown function; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HRF19193.1"
                     /translation="GDMLPGENRSSTIAIGHGKKAAKYINGYLMSNPYQKPDKHPTAS
                     FRKLHMWYKTEAPQREQNKLAPAVAVKNFEEIVSGLSEPEARFEAQRCLSCGNCFECD
                     GCFGACP"
     assembly_gap    3693..3736
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3737..3856
                     /locus_tag="PK977_13555"
     CDS             <3737..3856
                     /locus_tag="PK977_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF19194.1"
                     /translation="NRYKFNYDACTGCAVCYEQCPCHAIEMIPEPVNSTTDVK"
     gene            3846..>4307
                     /locus_tag="PK977_13560"
     CDS             3846..>4307
                     /locus_tag="PK977_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRF19195.1"
                     /translation="MSNKNKLVATIDGNEAAAYVAYRINEVCAIYPITPSSTMAELAD
                     EWASKGVKNIWGNVPDVIEMQSEGGAAGTVHGALQTGSLTTTFTASQGLMLMLPNMYK
                     IAGELTPAVFHVAARSLAAQGLSIFGDHQDVMAARTTGFAMLSSASVQEAHD"
     assembly_gap    4308..4323
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4324..>4877
                     /locus_tag="PK977_13565"
     CDS             <4324..>4877
                     /locus_tag="PK977_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRF19196.1"
                     /translation="QVASLKSRIPVVHFFDGFRTSHEVNKLELLSDEQISSMVPDELV
                     IAHRKRALNPENPFIRGTAQNPDVYFQGRETVNTFYNAMPGIMAEVMKDFEKLTGRKY
                     ELFEYTGAAEAEHITVIMGSGSETVEETVKALNKDGAKTGVIVVRLYRPFSLEHFVKV
                     LPATVKSIAVLDRTKESGAAGEPM"
     assembly_gap    4878..4915
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4916..>6007
                     /locus_tag="PK977_13570"
     CDS             <4916..>6007
                     /locus_tag="PK977_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRF19197.1"
                     /translation="QQGEIKSLPKIFGGRYGLSSKEFTPAMVKAVYDNLKKDNPKNGF
                     TVGINDDVTFTSLNYDPAYKLDESEWRQGLFFGLGADGTVGANKNTIKIIGENTDLYA
                     QGYFVYDSKKSGARTVSHLRFGPKPIRAPYLISKADFIACHQFNFVEKVEMLEFASEG
                     ATFLLNSPYSKEGVWDKLPGQVQKQLIDKKIKLYVIDATTVARETGMSGRINTIMQTC
                     YFALSGVLPREDAIKEIKKAIEKTYYKKGQKVIDQNFKAVDATLEHLYEVNYPKKVSA
                     KDVNLLTVSDKAPAFVKEVTAMMMAGHGDDIPVSKMTIDGTYPSGTTQWEKRNISDLV
                     PVWEPDVCIQCGNCAYVCPHSVIRAKFYHK"
BASE COUNT         1736 a         1105 c         1387 g         1614 t
ORIGIN      
        1 cttcaggagc aatcagtgtg ggttattgtt tttaggtaat atcaggtggg gatctatttc
       61 tttttttcag gaagggtaat agtataggca gtttcatatt ttacctcggt catttcgaaa
      121 ccgctttgca cagtgccgat attaggcagc ttggctagtt tgtttacaat gaagtcctgg
      181 tattcattca tgtcttttac aacgattttc agcaggtaat cataaatgcc tgtcatgtga
      241 tgacattcca tcacttcgga aagttttaca atatcccttt caaatgcttt cagcataggg
      301 tgtgaatgtt ctttcagctg cacattggtg aatgctacga ggtttttatc aatcttgttt
      361 ttatctaata cagcaacata ccgctgtata tatccttcct gttccagcca cttgatccgc
      421 tcataaacgg gggtgacgga tttgccaagc ttatcagcaa tttctttatt ggtcagtctt
      481 gcatcatgtt gcagtagctg caggatgttt atgtctgtat tatcgagctg taccatggca
      541 gaaaaatttt ctgtttagaa gcctttatga cttaaataat atctttatat tccaattagt
      601 gctataaaaa tagatttatt ttctaataaa attggaatca tgagaaaatt aatccatgtc
      661 ttcgattttt gcagtgtaaa acaaactgta tggattactc ccgtttttct cccgaaagcc
      721 tgatgatgag ttatggttat aaccctcatc tcagcgaagg tgccattaag tgccccattt
      781 ttcagacatc tacttttgtc tttcatagcg cagaagaagg aaaagctttt tttnnnnnnn
      841 nnnnnnnnnn ccatcgtaaa aagaaaaaac atgaaaagat agggctaatc tatagccgga
      901 tcaataaccc ggatatggag atactggaag accggttaac gctttgggat ggtgctgagg
      961 cctgcgccgt ttttgaaagc ggaatgagtg ctatttcaac taccatgctg gaatttttaa
     1021 aaccgggtga tttgttatta tacagcaacc ctatttatgg tggcacacac catttcattc
     1081 accatgtgct gaccaaattc ggcattcaca ttatgggatt cagggcagga gaaacaatgg
     1141 atgaaattga aatgcggtta aaaggatccg gtttgtctga taaactggga atggtttacc
     1201 tggaaacacc tgccaatcca actaatgatc ttatcgacat tgaaatgtgt gtggcattgg
     1261 cccgcaaata ttctgttgct gataaaaatg taatcgttag tgttgataat acttacattg
     1321 gtccaatttg gcagcatccg ctgaagaacg gagccgacct ggttttgtat tcagctacca
     1381 agtacattgg aggccacagt gatgtgatag ccggagctgt tttaggcaac aaagagttga
     1441 ttggccgggt aaaagtgctt cgaacattcc ttgggaatat gatcagtcct tatacaggct
     1501 ggttattgct gcgcagtctt gaaacgctga aagtgagaat ggacaaacag gcagaaaatg
     1561 cgcaaaaagt agtggattgg ctcaaacagc atccgctggt tgaaaaagta tattatctcg
     1621 gttgcctgga gcacagtaat ccctctcaac atgccattta cagaaagcag tgccttggta
     1681 acggagcgat gctaagcttt gatataaccg gaggtgaaaa ggaggcattt acttttctca
     1741 ataacctgaa actgattaaa ctggcagtta gccttggcgg cactgaaagc ctggcggagc
     1801 atccagctac gatgacccat gttgacattc ctgatgagga aagggcacac tataatatta
     1861 cttcaaaact tattcgttta tcaattggtg tagaaaatcc acaggatctg atatgggata
     1921 tcgatcaggc cctggaaaaa gtggctcagc tggatcatat atacaataag gaagtagcct
     1981 tctagcagtt ataaaatgag ttaagtgaaa gacagtcgct gccaaccagc ggctgttttg
     2041 cttaatatga tatgaatcag tggttttgct gaattaggtc acctgtaacc aactcattca
     2101 tttctaattt tcaccacttt ttgataccta ccatgtgtca aaaaccaaca ttatttaccg
     2161 ttgaaataaa cgaacatgtc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nncttcttac ccatgctgaa ggtacaggac ccaaacgttc ccgccgcccc gtttatatgg
     2281 atttcatgcc gccttgtaac agtgcctgcc ctgcaggtga aaacatacag gcatggatgg
     2341 cgcatgctca atcaggaaat tattttgaag cctttcaaac cattttggaa gacaatccct
     2401 ttccggccat tatgggccgt gtttgtgtaa aaccctgcga aacgggctgc aaccggaatc
     2461 acatcgagag tacagtgaac attcatgcag tggaacgctt catcggcgat gaggccatcc
     2521 gccaaaaatg gccggtgcag tatatgtatg aagaaacagg aaaaagaata ctggtgatcg
     2581 gtgcgggtcc gggtggatta tcagcagctt accatctggc gaggatggga cattctgtag
     2641 aaatatttga tgcaggaaaa catcccggcg gattattgtg gacaggtgtt cctgagtatc
     2701 ggcttcccaa agaaatactg gatgccgaag tggaacgtat cgtaaaaatg ggagtaaaga
     2761 tcaggctcaa ttataaagta aaggatgtat taacagaaaa gcatgccgga aattttgatg
     2821 ccgtttattt atcaatcggg gcacagctga tacatgatga agggtttgta catgatagct
     2881 cagtgtatat cacagatgct ttttctttct tcagcgaatc caaactgaac aactctcctt
     2941 ttgtacaaaa gaaagtggtt gtgtatggtg gaggtaaact ggcactgtac ctctcccgga
     3001 tattgaaacg ttacggttct gaagtatctg tttattatcc cggcgataag aaaatgatgc
     3061 cggcatatga ttacgaaact gaagatgcat tggccgaagg tgtggatgta cagttgtacc
     3121 gaagtatcag caagatcgaa aagaaaaaga tcaccctgga gaaaatgaaa gtggagaaga
     3181 ataaagctgt aggaacaggt gagtttgaaa cggtcgatgc agatgtactt attaatgctt
     3241 accggcagga gacagattct ggtttccttc gttcagtgtc agcaattaaa gtaaatgaag
     3301 acggaacagt ggtcattgat gcacaacgga tgacaggtca tgaaggaatt tttgcnnnnn
     3361 nnnnnaggag atatgcttcc aggggaaaac aggagttcta ccattgccat cgggcatggt
     3421 aagaaagctg caaaatatat caatggttac ctgatgagca atccttatca gaagccggac
     3481 aaacatccta cggccagctt tcgtaagctg cacatgtggt ataaaacaga ggcaccacag
     3541 agggaacaga ataaactggc gcctgcagta gcagtaaaaa attttgaaga aatagttagc
     3601 ggattgtcag agcccgaagc aagatttgaa gcgcagcgtt gtttaagctg cggaaactgc
     3661 tttgaatgtg atggatgttt tggcgcttgc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnaacc gttataaatt caactacgat gcctgcactg gttgtgcagt
     3781 gtgctatgaa cagtgcccct gtcatgctat agaaatgatt ccagaacctg ttaattcaac
     3841 tactgatgtc aaataaaaat aaattagtcg caaccataga tggcaacgaa gctgcagcat
     3901 atgtagcgta tcgtatcaat gaagtatgtg ccatttatcc catcactcca tcctctacta
     3961 tggcagagct ggcagatgag tgggcatcaa agggagtaaa gaatatctgg ggaaatgtac
     4021 ctgatgtgat agaaatgcag agtgaaggcg gcgcagccgg tacggtgcat ggcgctctgc
     4081 aaaccggttc gctgaccacc acttttactg cttcacaggg tttgatgctg atgttgccca
     4141 atatgtataa gattgccggt gaattgacac ctgcagtatt tcatgtagca gcccgttctc
     4201 tggctgcaca gggtctttcc atttttggtg atcaccagga tgtgatggcg gcaagaacga
     4261 cagggtttgc tatgctaagt tctgcttctg tacaggaagc acatgatnnn nnnnnnnnnn
     4321 nnncacaggt agcttcgctt aagtccagga ttccggtagt acacttcttt gatggtttcc
     4381 gcacttcgca tgaggtaaat aaactggagt tactgagtga tgaacagatc agttcgatgg
     4441 tgccggatga actggtgatt gcccatcgca aaagagcgtt gaacccggaa aatcctttca
     4501 tccgtggtac agcccaaaat cccgatgttt atttccaggg tagagagact gttaatactt
     4561 tttataatgc tatgccgggc attatggctg aagtgatgaa agattttgag aaacttaccg
     4621 gaagaaaata tgaactattt gaatatacag gcgctgcaga agccgaacac attacggtaa
     4681 ttatgggttc cggaagtgaa acagtggaag agacggtgaa agcgttgaat aaagatggtg
     4741 caaaaacagg tgtgatcgtt gtaaggttgt acaggccatt ctcgctggaa cattttgtaa
     4801 aggttttacc tgcaactgta aaatcaattg ctgtattaga cagaaccaaa gaatccggtg
     4861 cagcaggtga accaatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagca
     4921 gggcgagatc aaatcgttgc caaagatatt tggtggccgt tatggtcttt cttccaaaga
     4981 atttacaccg gcaatggtaa aagcggtata cgataacctg aaaaaggaca acccgaaaaa
     5041 tggcttcact gttggaataa acgatgatgt gacctttacc agtcttaact atgaccctgc
     5101 ttataagctg gatgaaagcg aatggagaca aggtctattc tttgggttag gcgctgacgg
     5161 tacagtaggt gccaataaaa atacgatcaa aataatcggt gagaatactg acttatatgc
     5221 gcaaggttat tttgtttatg attctaaaaa atcaggtgca agaaccgttt ctcatctgcg
     5281 gtttggcccc aaaccaatca gggctcccta tcttatctcc aaagcagatt ttattgcctg
     5341 ccaccagttc aattttgtag aaaaagttga gatgctggag tttgccagtg aaggagccac
     5401 tttcctgttg aacagccctt acagcaaaga gggggtgtgg gataagttgc cgggtcaggt
     5461 acagaagcag cttattgaca agaaaattaa attgtatgtt attgatgcta ctacggtggc
     5521 aagagaaaca ggtatgagcg gacgtatcaa tactattatg cagacctgtt attttgcttt
     5581 atccggagtg ttgccaagag aggatgccat caaagagatc aagaaggcca ttgaaaagac
     5641 ctattataag aaagggcaga aagtaattga tcagaacttt aaagcagtag atgccacact
     5701 ggagcatttg tatgaagtca attatccgaa aaaggtttct gcaaaagatg taaacctgct
     5761 cacggtttct gataaagctc ctgcatttgt gaaagaagta acggctatga tgatggctgg
     5821 tcacggggat gatattccgg tgagcaagat gacgattgac ggcacttatc caagcggaac
     5881 tacacaatgg gagaagagaa atatttctga cctggtgcct gtatgggagc ctgatgtatg
     5941 tatccagtgc ggtaattgtg cttatgtatg tccgcatagc gttatcaggg ccaagtttta
     6001 tcataag
//
LOCUS       DAORLS010000193         5903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7324_length_5903_cov_1.768810, whole genome shotgun sequence.
ACCESSION   DAORLS010000193 DAORLS010000000
VERSION     DAORLS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7324_length_5903_cov_1.768810"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK977_13575"
     CDS             complement(<1..200)
                     /locus_tag="PK977_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19198.1"
                     /translation="MEKDTLTLTPAVTDATEGNYNLFAKTDLLNNIIELDKTNNTGIS
                     AIPIYVKAKELKLGIPELNTLQT"
     assembly_gap    210..381
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<382..3806)
                     /locus_tag="PK977_13580"
     CDS             complement(<382..3806)
                     /locus_tag="PK977_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRF19199.1"
                     /translation="MANSRNAYVKRNSICDPVYTNEQQLSITTSNNTTIGPVTGMLPA
                     NGIIDQQLPLRLSWIPGINTVNYDLYVWDSAAAEPLIPYISNIAAISYTLPKNAPFPY
                     NKTYKWRVVSKNPCAQIPGPVQTFRLIPLPDLVISDIQAPATATSGQTITISWKVTNI
                     GPGKTLLNQSWEDGIYFALDTVPNVNLGNPFPFPGSWSQLTAAGRPLLLGKKDRPAAL
                     DSGQFYTNSIDFTLPLGYNFPVYVYGIAANNSSIYRILQVSNLNDTARAPNQIIITQP
                     PVPDLRVDSVFTPASIFSGSTMNITYKVKNYGVVTPAGGQWVDSFFISQNPLFDRNQC
                     IPLNAPKPNGSYYPNAAGATVYNTIQLNTDSSTTKNQQVVIPNFIFGTWFIYVKTNAG
                     TTTGNYIYEGALNNNNVNQAQLQVYLTPTPRLTINSLIVPVTTASTTQPIGVNWNIKN
                     EGFQDNIERNRGHYITMSTCRVPCPPGSQNNNSICIGPSVIRDSVVFGSSYWVDRVYL
                     STDPGGLNTANAVLVKEVKHGTENSGVYADAPAPTYSFVSCPALASGNINISNVIQPA
                     ANFPKAENFTIPSNLPPGNYYVYVYTNPTKKVFEYPGTPQIKRSDLPIVIQRPDAIVS
                     SISCPSSASGGQTIQVNYAITNNGPGAVFNHQRSDRFYISSNPVFDGSATVITTQTFT
                     ENLPVGTAVPHTFSYTIPPATTGTKYFYVVTNYDSLKETNQTNNTSAAAAIAISAAPP
                     ADLMVSSVHLQDSVLIQSPALFKYTVINSGTGSTIGNWTDSIFLSCSPVFNASTAKFI
                     AKRTQTRLVLPSGNYTDSFTLVIPKMSYEINNCFPQQLYAQGYFYVKANADSGAYEGA
                     ALNNNINGSGGRVVINPLVDHVLVAASVNRDTVSVGTPFSASWKVKNTGYKPIEAYYY
                     FYDDGIYFSPDSLVNTNDVQAFGFRNYNTINRNDSLVTTKSVMPPDLPTGDYYVIAKT
                     NYSDRIPAEKVFGNNYDFVRTISGEAKKVHLVRPPLPDLVDTILSAPASVAMGQPFSV
                     VYKITNIGSAVTFPGAWRNDLYLSADFQLQVGDRLLTGKTRTTPLAPGASFIDTVTCT
                     MPLNLAPGNYVLIAKANSNFAVTESDLTNNLGFGVIEAQLPDSTDLLVENVMRP"
     gene            complement(3731..>5903)
                     /locus_tag="PK977_13585"
     CDS             complement(3731..>5903)
                     /locus_tag="PK977_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19200.1"
                     /translation="TGSSSSSPTLLTAAGNLLFFVATDGTTGNELWKSDGTAAGTVLV
                     ADIVPGLGTPNIQTPVNVNGTLFFRATVSGFGTELWKSDGTAAGTVLVKDIIAGTGSP
                     GIDDMIAYNGKLFFSANDGVNGNEPWVSDGTAAGTILFVNIATSGTFPNSNPTNFAVA
                     NNLLYFTAFTASTQFEPWVSDGTPGGTYMITEIVPGGSGGGGFGFVYMNGYVYFDANG
                     PLGHELWRTDNTAAGTTLVKDINTGSGDGNPQQLTVVNNTLFFRATTSTGGVEFYRSD
                     GTAAGTVSYDLYPGTTASNPDYLTALNSKLIFTATHPTLNREIWRAIAPPHSSFTVVG
                     DTTPCVGGTAVYAAANLVNDGYTFNWSLPLGGGTIISAADTVVVQWDTAGSRSVQLVL
                     SNIAGSSAPKQRELVVSGTAPDRVPVIFNFARNLSVDSVLQSASLQWFRNDTAISGAT
                     SPSYYAGLAGSYTLKYKNNCGYGPSSNAFSFPADTLSQTITFPHTDTIAMSPAAKIVL
                     PATASSGLPVFYQKMSGPGNVVNDTLFISGVGTIIIKALQPGDDVYSAAAPVYDTIIV
                     KKGSQYISFDSIPPKIYPEAAFPLTAISSVGLPVSFSVVSGPASVANGNLTVLGAGLI
                     TVRATQSGDSNYNAAVPVDRTFCAGVRTLSAITGITAPCFATYQYNTQKIIGANYTWT
                     LSGGGILTTSNDTAWVQWQTAGTHTLSVTASATRCTQMNSN"
BASE COUNT         1561 a         1209 c         1259 g         1702 t
ORIGIN      
        1 gtttgaaggg tattcagctc cggtataccc agcttcagtt cttttgcttt gacataaatg
       61 ggaatggctg aaatgccggt attatttgtt ttatccagtt caataatatt attcagcaag
      121 tctgtttttg caaacaggtt ataattacct tctgtagcat cagttactgc cggtgtcagc
      181 gtcagtgtat ctttttccat aggcaataan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nggcctcatt acattttcaa caagcaggtc agtagaatcg
      421 ggcaactgtg cttctattac accgaagcca agattgtttg ttaaatcact ctccgtaaca
      481 gcaaagttgg aatttgcctt agctataagt acgtagtttc ctggggctaa gttcaaaggc
      541 atggtacatg ttactgtatc aataaaggat gcccccggtg caagcggagt tgtcctggtt
      601 ttaccggtaa gcagcctgtc gccaacctgt aactggaaat ctgcggataa atacagatcg
      661 tttctccatg cacccggaaa tgtaacagca gatccaatat tggttatttt ataaacgaca
      721 ctaaagggtt gtcccattgc cactgatgca ggggctgaca ggatggtatc aactagatca
      781 ggtagcggtg gtcttaccag gtgtactttc tttgcttcac cgcttatggt tcttacaaaa
      841 tcgtaattgt tgccaaatac tttttctgcc gggatacggt ctgaataatt tgttttagca
      901 ataacatagt agtcgcctgt tggcaaatct ggcggcataa cagattttgt agttaccagg
      961 ctgtcattac ggtttatggt attataattt ctgaatccaa atgcctgtac atcgttggta
     1021 ttaaccaggg aatcgggtga aaaatatata ccatcatcat aaaaataata ataggcctcg
     1081 attggcttgt acccggtatt tttaactttc caggaagcgc tgaaaggcgt cccaacagat
     1141 actgtatccc ggtttactga ggcagcaacc aatacatgat ctaccaatgg atttattacc
     1201 accctgccgc cacttccatt aatattattg ttgagagctg ctccttcata tgcgccgcta
     1261 tcggcatttg cctttacata aaaataacct tgtgcataga gttgttgcgg gaaacagtta
     1321 ttaatctcat aactcatttt aggaatcaca agagtaaatg agtctgtata atttcctgaa
     1381 ggcagaacca atcttgtttg cgtacgcttc gctatgaatt ttgctgtgga agcattgaaa
     1441 accgggctgc aactgaggaa tatactatct gtccagttac cgatcgttga cccggtgcca
     1501 ctgtttataa cagtatattt aaacagtgcc ggtgattgta tcagtacaga atcctgaaga
     1561 tgtacagagg ataccatcaa atctgcggga ggcgctgcag atatagcaat tgctgctgca
     1621 gcactggtat tattggtttg attggtttct ttaaggctgt catagttcgt aacaacataa
     1681 aaatattttg tgcctgttgt agccggggga atagtataac tgaatgtgtg gggcacagca
     1741 gtaccaacgg gcaggttttc cgtaaatgtt tgtgtagtta tgactgtggc agaaccatcg
     1801 aagaccggat tactactgat atagaatcgg tcactacgct gatgattgaa tacagcgccg
     1861 gggccattgt tcgtaatagc atagttaacc tggatggttt gtccgcccga agctgaagat
     1921 ggacaactga tggatgatac aatagcgtcc ggtctttgta taacgattgg caaatcgctt
     1981 cttttgattt gcggtgtacc cggatattca aatacctttt tggtaggatt agtataaaca
     2041 tacacgtagt agtttcctgg cggaaggttg gaaggaatag taaagttctc tgcctttggg
     2101 aagttggccg ccggttgtat cacattagat atgttgatat tgccacttgc caatgccggg
     2161 cagctaacaa aactgtaggt aggcgctggc gcatcggcat aaacacccga attctctgtg
     2221 ccatgcttta cctcctttac caatactgca ttggctgtat ttaatcctcc cggatctgta
     2281 ctgaggtaaa ctctgtcaac ccagtagctg ctgccgaata caacactatc cctgattaca
     2341 gaaggaccta tacatatact gttattgttc tggcttccgg gcggacatgg taccctgcat
     2401 gtactcattg ttatataatg ccccctgttc ctttctatat tatcctgaaa gccttcattc
     2461 tttatattcc agtttacccc aataggctgt gtggtgctgg cagtagtgac aggaacaatt
     2521 aacgaattaa tagtcagcct gggcgtaggt gtaaggtaaa cctgcaactg tgcctggttc
     2581 acattattgt tattcaatgc cccctcataa atataattgc ctgttgttgt acctgcattt
     2641 gtttttacat aaataaacca ggtgccgaat ataaaattgg gtattaccac ctgttgattt
     2701 ttggtagtac tactgtccgt atttaattga atagtattat acaccgtagc acctgcagca
     2761 ttaggataat aagaaccatt gggcttgggt gcattcagcg ggatacattg atttctgtca
     2821 aataatggat tttgtgaaat aaagaatgaa tcaacccatt gccctcctgc aggtgttacc
     2881 actccatagt ttttcacttt ataggtaata ttcatggtac tgcctgaaaa aatggatgct
     2941 ggagtaaata cagaatcaac cctaaggtca gggacaggtg gttgtgtgat aataatctgg
     3001 ttgggcgccc tggctgtatc attcagatta ctgacttgta aaattctata aatggagcta
     3061 ttattggctg caatgccata tacatagacc gggaagttgt aacccaatgg caatgtaaaa
     3121 tctatgctgt tggtataaaa ttgtccgctg tccaatgcag caggcctgtc ttttttacca
     3181 agcaataacg gccttccggc agcagttaat tggctccagg atcctggaaa agggaaagga
     3241 ttaccaaggt taacattagg aacagtatcc aatgcaaaat aaattccatc ttcccatgat
     3301 tggttcagca atgttttacc ggggccaata tttgttactt tccagctaat ggttatcgtc
     3361 tgcccggacg tagctgttgc aggtgcctga atatcgctga tcaccagatc aggtaatggt
     3421 atcaacctga atgtttgtac aggacctggt atttgtgcac aaggattttt tgataccact
     3481 ctccatttat acgttttatt atacgggaac ggggcgtttt taggaagggt atatgaaatc
     3541 gcagctatat tggatatgta tggtataagc ggttcagccg ctgcagaatc ccatacatac
     3601 aggtcatagt ttactgtatt tatgccaggt atccatgata agcgtaatgg caattgctga
     3661 tcaataatac catttgcagg taacatgcct gttaccgggc cgatagtagt gttattggat
     3721 gtggtaatac ttagttgctg ttcatttgtg tacaccgggt cgcagatgct gttacgctta
     3781 acgtatgcgt tcctgctgtt tgccattgta cccacgcagt atcattgctg gttgtcaaaa
     3841 ttccgccacc actcagggtc catgtataat tggctcctat aattttttgt gtgttgtact
     3901 ggtaagtggc gaaacaaggg gctgtaattc ctgtgatggc actcaatgtt cttacacctg
     3961 cacagaaggt tctgtccacg ggtaccgctg cattataatt tgaatcaccg ctttgtgttg
     4021 cccttactgt tattaatccc gcaccgagta cggtaaggtt tccattggca acagaagccg
     4081 ggccggatac tactgaaaag cttactggca agccgacaga tgaaatggct gttagcggga
     4141 atgctgcttc aggatagatt ttgggcggaa tagaatcaaa gctgatatac tgacttcctt
     4201 ttttaacaat gatcgtatca tacacaggtg cagcagcact atatacatca tcaccgggtt
     4261 gtaaagcttt tataatgata gtaccgacac ctgatataaa caatgtatca ttcactacat
     4321 taccgggtcc actcattttc tgatagaaaa caggaagacc ggagctagct gtagccggca
     4381 aaactatttt ggctgctggc gacatggcaa ttgtatcagt atgagggaaa gttatcgttt
     4441 gtgaaagggt atcagccgga aagctaaaag cattcgaaga ggggccataa ccgcaattat
     4501 tcttgtattt aagtgtatag ctaccggcga ggcctgcata ataggaaggg ctggtagctc
     4561 cactgatagc tgtatcattc ctgaaccatt gcagcgaggc gctttgcaac acagaatcca
     4621 cactaagatt gcgggcaaaa ttgaatatta ccggaacccg gtcaggtgca gtaccgctta
     4681 ccactaattc tctttgtttt ggagcggaac ttccggcaat atttgaaagc accagctgca
     4741 cacttctgct acctgctgta tcccattgta ccactacagt atcggctgca ctgataatag
     4801 tacctccgcc caatggaagg ctccagttaa aagtataacc atcattcaca agattagctg
     4861 cagcataaac agccgttcct ccaacacaag gcgttgtatc acccacaaca gtaaaagaag
     4921 aatgaggagg tgcaatcgct ctccagattt ccctgttcag ggttgggtgg gtagctgtaa
     4981 aaattaattt actgttcagc gccgttaaat aatcaggatt agaagctgtt gttcccggat
     5041 atagatcata tgataccgtg cctgctgctg taccatcact tctataaaac tctacaccgc
     5101 cggtggatgt agttgctcga aagaataatg tattgtttac tacggtaagc tgttgcggat
     5161 ttccatcacc tgaaccggta ttaatatctt ttaccaaagt ggttccggct gcggtattat
     5221 ctgttcgcca gagttcatgt cctaaagggc cattggcatc aaaatacaca tacccattca
     5281 tgtatacaaa accaaagcca cctcctccgc ttccgccagg tacaatttca gtaatcatat
     5341 atgtcccccc gggtgtgcca tcgcttaccc atggctcaaa ctgggtgctt gcagtaaaag
     5401 cagtgaaata caataaatta ttggctaccg caaaattggt tggattggaa ttagggaaag
     5461 ttccgctggt tgcaatattt acaaacagaa tagtacctgc tgctgttcca tcacttaccc
     5521 agggttcatt accgttcact ccgtcgttgg cagagaaaaa taattttcca ttatatgcaa
     5581 tcatatcatc tattcctggt gaaccagttc cggcaatgat atcttttacc agtactgtac
     5641 ctgctgcagt tccgtcgctt ttccataatt cagtaccaaa gccagatact gttgcacgga
     5701 aaaataatgt cccgtttaca ttcaccgggg tttgaatatt gggagtacct aagcctggta
     5761 cgatatcagc aaccaagact gttcccgcag cagtaccatc gcttttccat agttcattgc
     5821 cagtagtgcc atcggtagca acaaaaaata gcaggttgcc tgcagccgta agtagtgttg
     5881 gcgaagaact gcttgagcct gta
//
LOCUS       DAORLS010000194         5902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7328_length_5902_cov_2.240465, whole genome shotgun sequence.
ACCESSION   DAORLS010000194 DAORLS010000000
VERSION     DAORLS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7328_length_5902_cov_2.240465"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>2336
                     /locus_tag="PK977_13590"
     CDS             259..>2336
                     /locus_tag="PK977_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF19201.1"
                     /translation="MKFLKQSLLAFLLLAFSVAKADEGMWLPQLLAQLNEKQMKSMGM
                     KISAADIYNINKGSLKDAIVSFGGFCTGEVISSKGLVLTNHHCGFDAIQNHSTIQNNY
                     IRDGFWARNNAEEIPNPGLFVTFIIRIDDVTKQVLNGVTKNMDEKERQSAIDKNIAEL
                     RKNVKKESYQDSFIRAFFEGNQYFLFVTETYKDVRLVGAPPSSIGNFGKDTDNWMWPR
                     HTGDFSMFRIYAGKDNKPAEYSVDNVPYVPKRSLSISLDGVAENDFTMVFGFPGRTTQ
                     YLHSAAVEQIMKVNDPVKIAIRDKALAVIDGFMRKDEQIKIQYAAKYAGIANSWKKWQ
                     GEVLGLTSTNALGKKQAYEAEFQKRVNANTQWKEAYGNLLLDLAKTYNEFEKYSLARD
                     FFNETMSRIEAFAIVNQLNSLRNAKEKNGEKGFNNALPGVKERLAEIYKEYSPVVDKQ
                     LFIALMDKFVKDQPNEFVAPYLLNKLKENNNDISKLADELYNNVDLFQNGNKVLQQLS
                     SNPEVVMAANDMNHANQLIAELSNYYNSTVSKNITGLQSRINQLNRTYMQAQMEVMKE
                     KKFYPDANSTLRVTYGNVKGYNPRDAVQYDYYTYLDGVMDKYKPGDYEFDLPAKLRDL
                     YKNKDYGQYGSNGKMPVCFIARNHTTGGNSGSPALDAYGNLIGLNFDRVWEGTMSDIN
                     YDPSICRNIMV"
     assembly_gap    2337..2376
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2608..4770
                     /locus_tag="PK977_13595"
     CDS             2608..4770
                     /locus_tag="PK977_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRF19202.1"
                     /translation="MSIIQTIRDKGAKVSVVLIALALVGFILTDYFSGRGRGGFSGSN
                     TVGKVNGKSIGFEDFNKRVEQMEANMKQQYGANIDQAILNSQAVEETWNQEINRVLLY
                     GEIDKLGFQIGKKELGDILYGPNAPADLKRQFSDEAGNYNAVLAKQNIDQMLKKGTAE
                     QKENINNYINELEQIRKSEKFISLLTNSVNFPRWFVEKQNADNSQIANISMVRQTYAT
                     VPDSTIKISDKEISDYISKHKDDFKQEESRSISYVTFSAAPSVADSTETRNSLLSKKD
                     AFAAATDMEQFLMSEGATNYYNGYISGKTIQVAVKDSIFRTPVGGVYGPYLDGNSYVL
                     AKLEGVRTMPDTVNLRHILVATMQRDQQSGAMYPVRDSATAKNLIDSIRNAINTGSNF
                     DTVCAKLSDDPGSKDKGGVYENVYSGQMVAAFNDFIFLNPVGSKGVVKTEFGFHYIEI
                     LSQKGSGAAYKVAYLPKEIIASQETDSKAQNDANMFAADSRDVKSFDEGFEKNWKPKG
                     YVKGIATNIPRIGAEIRGVGYSRNFVRSIYDAKRGEVLQPERIDDNYVVAVVTEVIEK
                     GTMGVAQARSSVEPLLRNKKKAEILKQKIGKVTTLEAAAQVMGNAPIEPVDSLRMSSR
                     NINPKLGYEPKVVGAAFNPANKGKVVPEVLDGAYAVYVIRVDNVSATPSEGTTVADQR
                     KLLADQVKQYINNQQSPANPVNALKKAATIVDKRADRY"
     gene            4887..5399
                     /locus_tag="PK977_13600"
     CDS             4887..5399
                     /locus_tag="PK977_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HRF19203.1"
                     /translation="MSEPFVNKVAESGLITLDLEKYYPKGETAVFDLKDHLFMGLILK
                     EKDFREALKNLDWSVYQDKNVALTCSADAIIPVWAWMLVVSYLQPVAKEVVMGDEKEL
                     HRSLYLKNIDAIDVNEFSGQRVVIKGCGETPIADYVYMEITKKLRPVVKSIMYGEPCS
                     TVPLYKAKKD"
     assembly_gap    5516..5641
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5642..>5902)
                     /locus_tag="PK977_13605"
     CDS             complement(<5642..>5902)
                     /locus_tag="PK977_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19204.1"
                     /translation="SVGSIIHPLYLYKNYFGFGAFAGYPSCPFPYDLGLACKWTNNMK
                     EFQSCTYVLALANGCFFKLDSCNYWNNCGGINELSAIKKVTLS"
BASE COUNT         1873 a         1213 c         1250 g         1400 t
ORIGIN      
        1 acaccagaac acttttaacc ggtccgatga aaacttcatt cctaaacttt cagtttcata
       61 tccacgtttc acatcaggat atccacataa aattatcttc attcacaacc cggtggggag
      121 aaaaaacgat cagtcagcct tctattgact gtttaaaaaa caatcaacat gactaaagtc
      181 atacacttta ctaaatatat aagcggaata agctttattg atgaatgacc taattttaca
      241 gctactaaaa ttttccatat gaagtttctt aaacaatcat tgcttgcttt tttactcctt
      301 gctttttctg tagctaaggc cgatgaagga atgtggcttc cgcaactgct ggcccagctc
      361 aatgaaaaac aaatgaaaag catgggtatg aagatcagcg ccgcagatat atataatata
      421 aataaaggaa gcctgaaaga tgcaatagtc agctttggcg ggttctgcac cggtgaagtg
      481 atttcttcca aaggactggt actaacgaat catcactgcg gttttgatgc tatccaaaat
      541 cattctacga tacagaataa ctatataaga gatggtttct gggcccgcaa taatgctgaa
      601 gaaattccca accccggttt atttgtcact ttcatcatcc gtattgatga tgtaaccaaa
      661 caggtgctga atggtgtgac aaagaatatg gatgaaaaag aaagacagtc tgctattgac
      721 aagaacatag ctgaactcag aaagaatgta aaaaaagaaa gctaccagga tagttttatc
      781 agggctttct ttgaaggcaa ccaatacttc ttgttcgtaa cagaaactta caaagatgtt
      841 cgcctggtag gtgcaccacc atcttctatt ggtaatttcg gaaaggatac tgacaactgg
      901 atgtggcccc gccacaccgg cgatttttct atgttccgca tctatgccgg taaggataat
      961 aaacctgccg agtattcggt agataatgta ccctatgttc ccaaacgttc tttatccatt
     1021 tcacttgatg gtgtggcaga gaatgatttc accatggtat ttggttttcc cggaagaacc
     1081 acacagtatt tacattctgc agcggtagag cagatcatga aagtgaatga ccccgttaaa
     1141 atagccatcc gtgataaagc attggcagtt atcgatggtt ttatgcgtaa agacgagcag
     1201 atcaaaattc agtatgctgc caagtatgcc ggtattgcca actcctggaa aaaatggcag
     1261 ggcgaagtat taggacttac cagcaccaat gcactcggaa aaaaacaagc ctacgaagca
     1321 gaattccaga aaagagtaaa cgcaaacacg caatggaaag aggcctacgg aaatttattg
     1381 ctggacctgg ccaaaaccta caacgaattt gaaaaatatt cgctggcccg tgatttcttt
     1441 aacgaaacca tgagccgtat agaagccttt gccattgtca accagcttaa cagcctgcgc
     1501 aatgctaaag agaaaaacgg tgaaaaaggg ttcaacaatg cattaccggg tgtaaaagaa
     1561 aggctggcag aaatctataa agaatacagc ccggtggtag ataaacaact cttcattgcg
     1621 ttgatggata aattcgtaaa agatcagccc aatgaatttg tagcacctta cctgctgaac
     1681 aagctgaaag aaaacaacaa cgacatcagt aagctggcgg atgaattata taataatgta
     1741 gatctgtttc agaatgggaa taaagtattg cagcaacttt cttccaatcc ggaagtggtg
     1801 atggctgcca acgatatgaa ccatgccaac cagttaattg ctgagttgag taattattat
     1861 aacagcacgg tttcaaaaaa catcaccggg cttcaaagca ggatcaacca gttgaaccgt
     1921 acttatatgc aggcgcagat ggaagtgatg aaagagaaga aattttatcc cgatgccaac
     1981 agcaccctgc gggtcactta cggaaacgta aaaggctata acccgagaga tgctgtacaa
     2041 tatgattatt acacctacct cgacggggta atggacaaat acaaacccgg cgattatgaa
     2101 tttgatttgc cggccaagct cagggatttg tacaaaaaca aagactatgg ccagtacggc
     2161 agcaacggca aaatgcccgt ttgtttcata gcccggaacc acactaccgg cggcaactcc
     2221 ggtagccccg cccttgatgc ctatggcaat ctcatcggcc tcaactttga ccgggtatgg
     2281 gaaggaacca tgagtgatat taactacgac ccttctatat gccggaatat catggtnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngata aatatgccgg ggccggccac
     2401 ctggttaagg aaatgaacct ggtacacccc aaaaagaaga aataagcccc aaccagctac
     2461 taaacaacgg ttttaccaaa tggaaaagct cccggcaggg ggcttttttt agcgattttc
     2521 cacagttttt cccctatttt tgcccgcccg tatcgagcct taaattttga acgggtcatt
     2581 ccttttataa aagaataata ctgtattatg tctatcattc aaacgatcag ggacaaagga
     2641 gccaaagtat ctgtggtatt gattgccctg gccttagtgg gattcattct taccgactat
     2701 ttctccgggc ggggccgtgg aggattttca ggttccaaca ccgtaggtaa agtaaatggt
     2761 aaaagcattg gcttcgaaga cttcaacaag agggtggagc agatggaagc caacatgaaa
     2821 caacagtatg gtgccaatat cgaccaggca attctgaaca gccaggccgt ggaagaaacc
     2881 tggaaccagg agatcaaccg ggtattgctt tacggagaga ttgataaact cggcttccag
     2941 atcggtaaaa aagaactggg cgacatttta tacggaccca atgcacctgc cgatctgaaa
     3001 agacaattct ctgatgaggc aggtaactat aatgcagtac tggccaaaca aaatattgac
     3061 cagatgctga aaaaaggaac agctgaacaa aaagaaaata tcaataatta tatcaacgaa
     3121 ctggagcaga tccgcaagtc tgaaaaattc atttcactgc ttaccaatag tgtgaacttt
     3181 ccccgctggt ttgtagaaaa acaaaatgca gacaacagcc agatagcgaa catctctatg
     3241 gttaggcaga catatgctac cgtaccggat agcactatta agatcagcga caaagaaatt
     3301 tcagattata tcagcaaaca caaagacgat ttcaaacagg aagaaagccg cagcatcagc
     3361 tacgttactt tcagtgctgc acccagtgtt gctgacagca ctgaaacaag aaacagcctg
     3421 ctgtctaaaa aagatgcatt tgctgctgca acagatatgg agcagttcct gatgagcgaa
     3481 ggcgctacta attactacaa cggctacatc agtggaaaaa caatacaggt agctgtaaag
     3541 gattctatct tcagaacccc ggttggcggc gtctatggtc cttacctcga tggtaacagc
     3601 tatgtactgg caaaacttga aggtgttcgc accatgcccg atacagtaaa cctccgtcac
     3661 atcctggtgg ccaccatgca gcgggatcag caaagtggtg cgatgtatcc ggttagagat
     3721 tctgctacgg ctaaaaactt aatcgacagt attcgtaatg ccatcaacac cggctccaac
     3781 ttcgatactg tttgtgcaaa actgtctgac gatccgggca gcaaggataa aggtggtgtg
     3841 tacgaaaatg tttattccgg ccagatggta gctgctttca acgatttcat tttcctgaat
     3901 cctgtaggca gtaaaggtgt tgtaaaaaca gaatttggtt ttcactacat cgaaatatta
     3961 tcacagaaag gaagcggcgc tgcttacaaa gtggcctacc tgcctaagga aattatcgcc
     4021 agccaggaaa cagacagcaa agcgcaaaac gatgccaaca tgtttgctgc tgacagccgt
     4081 gatgtaaaat cttttgatga aggttttgaa aagaactgga aacccaaagg ctatgtaaaa
     4141 ggaatcgcta caaacattcc ccgcattggt gccgagataa gaggtgtggg ttactccaga
     4201 aattttgtaa gaagcatcta tgacgcaaag agaggcgaag tactgcaacc ggaaagaatt
     4261 gatgataact acgtagtggc agtagtaaca gaagtgatcg aaaaaggaac aatgggtgtg
     4321 gcacaggccc gcagttctgt agaaccattg ttgcgcaata aaaagaaagc cgagatactg
     4381 aaacaaaaaa tcggtaaagt aacaacactc gaagcagcag cacaggtaat gggcaatgca
     4441 ccgatagaac cggttgacag cctgcgcatg agctcaagga atattaaccc caaactgggc
     4501 tatgaaccca aagtggtagg tgctgcgttc aacccggcca acaaaggcaa agtggtgccc
     4561 gaggtactgg atggtgctta tgctgtatat gtaattcgtg tagataatgt aagcgctact
     4621 ccttcagagg gtacaactgt agctgatcag cgtaaattgc tggctgacca ggtgaagcaa
     4681 tacatcaaca accagcaaag cccggccaac ccggtgaatg cgctgaagaa ggccgcaacg
     4741 atcgtagata aaagagcaga ccgctactaa acttctatac aaccaagacc aactgcaaga
     4801 gccctgtagc gaaagctgca gggtttcttt tttacgggat gcgcaaacaa cctcccgaac
     4861 ctatttgtta cttttacaat tgaatcatga gtgaaccatt tgtaaataag gtagccgaaa
     4921 gcggactcat caccctcgac ctggaaaaat attatccaaa gggagaaacg gccgtctttg
     4981 atttaaaaga ccatctcttc atgggcctta tcctgaaaga aaaagatttt cgggaagcac
     5041 tgaagaacct ggactggtct gtttaccagg acaagaatgt agcactcacc tgttcagctg
     5101 atgcgatcat tccggtatgg gcatggatgc tggtggtatc ttacttgcag cctgttgcca
     5161 aagaagtagt gatgggtgat gaaaaagaat tgcaccgcag tttatacctg aagaatattg
     5221 atgccattga tgtaaacgag ttttccggtc agcgggtggt tatcaaaggc tgtggcgaaa
     5281 cacccatcgc tgattatgta tacatggaga tcaccaaaaa actccgcccc gttgtaaaaa
     5341 gcatcatgta cggcgaacct tgcagtaccg ttcccttgta taaagcgaaa aaagattaat
     5401 gagtaatcgt tagtggttga gtaattttaa agttatctcc cgttaaagaa ataaagtata
     5461 aaccactttc caaactctcc gtttccaatc gaatttcgtt ttcgccaaga ataatnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 ngataaagta acttttttaa tggcgcttaa ttcatttatt ccaccacagt tattccaata
     5701 attacaggag tctaatttaa agaagcatcc gtttgctaga gcaagcacat aagtacaact
     5761 ttgaaattcc ttcatgttat tcgtccattt acaagctaat cccaaatcgt atggaaaagg
     5821 acagctagga tagcctgcaa aagcaccaaa gccaaaatag tttttgtata aatagagcgg
     5881 atgaataata cttccaacac tt
//
LOCUS       DAORLS010000195         5876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7380_length_5876_cov_2.188456, whole genome shotgun sequence.
ACCESSION   DAORLS010000195 DAORLS010000000
VERSION     DAORLS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7380_length_5876_cov_2.188456"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..>393)
                     /locus_tag="PK977_13610"
     CDS             complement(31..>393)
                     /locus_tag="PK977_13610"
                     /inference="COORDINATES: protein motif:HMM:NF036789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HRF19205.1"
                     /translation="GCNIDISKVAESGKVFLVKNSEIIDREVVNQFFNKTLFLRQKSS
                     DAKGWILDIMKCVDEIKTDIFNLDEIYKFEQKLKLKYPNNNFIKDKIRQQLQILRDKG
                     IIEFVGRGKYKKIKYGNI"
     assembly_gap    394..431
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<432..>781)
                     /locus_tag="PK977_13615"
     CDS             complement(<432..>781)
                     /locus_tag="PK977_13615"
                     /inference="COORDINATES: protein motif:HMM:NF017822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HRF19206.1"
                     /translation="NSQIARILTENWVLNNSYCPSCGYLPLNEFENNRPVADFYCKEC
                     SEEFELKSKGGKLTNTITDGAYSTMIERINSENNPNFFFLTYSKSWCVNDFLIIPKQF
                     FTEEIIIKRPPLAPT"
     assembly_gap    782..817
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1440..1832
                     /locus_tag="PK977_13620"
     CDS             1440..1832
                     /locus_tag="PK977_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HRF19207.1"
                     /translation="MWLPFLNIFFFIFHTALTLFNMSGWAFRKTRKWHLLTILLTAAS
                     WFILGIWYGWGYCACTDWHWQVREAMGINDRSNSYIHFLLLQLTGKDFDTNLVEKVTL
                     YVFLAASVLSIWLNLLDRRKAARPRKIV"
     gene            1870..3018
                     /locus_tag="PK977_13625"
     CDS             1870..3018
                     /locus_tag="PK977_13625"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF19208.1"
                     /translation="MRRFFLMSLLAASLVTLSLHSVAQPSRAEDSIATIMQQHEVMGL
                     AVAVVKKGKLVYTHSFGLKNKELNQPLQEENLFRIASISKSFSATAIMQLAEAKKLSL
                     DDDVSNLVGFTVRHPKFPNTIITLRMILSHRSAINDSQGYFTLDAINPAKNANWQKCY
                     SEYEPGTGYRYCNLNYNMAGTIIEKISGERFDKYIQKHILEPLGLYGGYWVDGLDSTR
                     FASIYEYDDSLKTLVAQPMAYAPRRQEIGSYVMGYSTPIFSPTGGMKISAPDLARYMI
                     MHMKMGKANGKRIIKKKSAITMQTPLSEKEGYGLAIATTDKLIAGKTLKGHTGSAYGL
                     YSAMYFDPNEKWGIVVITNGCRPSYTEGYNTVIRKTVNVLYNHIVSEK"
     gene            3477..3617
                     /locus_tag="PK977_13630"
     CDS             3477..3617
                     /locus_tag="PK977_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19209.1"
                     /translation="MRTRLLQASLLLFAGIIVFTSCVSQRKTGCDTTGKIKREIKFKG
                     FL"
     gene            3938..4435
                     /locus_tag="PK977_13635"
     CDS             3938..4435
                     /locus_tag="PK977_13635"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF19210.1"
                     /translation="MSLAQQFIAEIQQEAAATKRLLSIVPWEKGDYKPHEKSMTLKRL
                     ASHVAEINGWWKECLVQDELDFAKDSGERKEHADTGAMVAWHDELVQKAVAILQNTPD
                     EEFAKPWTMRAGEQIFFTLPKGEVVRTWCLNHLYHHRGQLTVYLRLLDIPLPGTYGPS
                     ADFQG"
     gene            complement(4448..>5506)
                     /locus_tag="PK977_13640"
     CDS             complement(4448..>5506)
                     /locus_tag="PK977_13640"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19211.1"
                     /translation="LALVKAAIDEAHKNNKRVAVHATERITAQLAVEAGADFLVHSVD
                     DEIVPDDFVKLLKEKKTVLCPTLVVAGNYGKVLGDTYDFTTDELKITHPELAGTIIDY
                     PLPDTALAGMYINFLKNPAQHQKQRQTDSVMAVNLKKLIDGGVIIATGTDAGNTGTQH
                     AASYFEELKAMQAAGLNTWQLLEASTINGAKAMGQEKEWGSIAQNKLANMLLLTANPL
                     DSLSNWRKVEWVMNRGAMFYPDSLITISPVDLVQQQLNAYNAHNLEAFLAPYAEDVEI
                     YQFPNTLLSKGKEQMRKDYLFITKVPKLFCRLQNRVVQGNMVIDHEEVSGFGPKPLHA
                     VAIYLIEKGKISKVYFQQ"
     assembly_gap    5507..5670
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5671..>5876)
                     /locus_tag="PK977_13645"
     CDS             complement(<5671..>5876)
                     /locus_tag="PK977_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF19212.1"
                     /translation="YRPYAEEIQWVHNHMEDFLRRYLSVGITSVIDVGATYNFLRQRD
                     SFTTKNYAPLIRITGPLLTTYVPEV"
BASE COUNT         1536 a         1304 c         1172 g         1626 t
ORIGIN      
        1 attcttgata ataactcttg aatttcaatt ttaaatgttg ccatatttaa tttttttgta
       61 tttaccacga ccaacaaatt caataatccc tttatcacgg agaatttgaa gttgttgcct
      121 tattttatct ttaatgaaat tattgttcgg gtattttaat ttgagctttt gctcaaactt
      181 atagatttca tcaagattga aaatatcagt tttgatttca tctacacact tcataatatc
      241 taaaatccaa ccctttgcat cacttgactt ctgcctaaga aacaaagttt tattaaaaaa
      301 ttgatttacc acttcacggt caataatctc agagtttttt actaagaaaa ctttgcctga
      361 ctctgccact ttggaaatgt caatattaca cccnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn ngttggagct aatggtggtc ttttaatgat aatttcttct gtaaaaaatt
      481 gtttaggaat aatcaaaaag tcgttaacac accaactctt tgaatatgta agaaagaaaa
      541 aatttggatt gttttcagag tttattcttt caatcattgt tgaataagct ccatccgtaa
      601 ttgtattcgt gagttttcca cccttactct ttaattcaaa ttcttctgaa cattctttgc
      661 aataaaagtc agcaacaggt ctattgtttt caaattcatt taagggtaaa tatccgcaac
      721 taggacaata cgaattgttt agaacccaat tttctgttag aattctcgct atttgagaat
      781 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt aaaggatagg ttcattttta
      841 tcaatggtaa ggcttcacct gcaccccttt ggtcatatgg aagtgaattg aatacagtat
      901 catgtctgta gtctttttct caaattgtat tattcatgat taatcattta ttgctgattt
      961 tattactaat tcgattgtgt tactaataac tgaaaattac ctatcatgct aatttcaaat
     1021 ttagtaagtt tcaatttata atttaacatt gggctgacat gtgatgttag actagctcag
     1081 tagacttacc gaacgtagat gagtgcactt cactgcgttc agtgtaaact tgcaaccaca
     1141 aaacatagta gtactacagt cccgacgcta acggtcggga tctgcgaccg gatatgctat
     1201 gctgcacaag ggctgccgga tggaagcgga aagcccgcag ccccgctttg attaaagatt
     1261 cctccttcgt cggaatgaca aaacggggcg aggacttgca gcggaaaatg atagtagtac
     1321 tacagtcccg acgctgaagg tcgggatctg cgatgctaaa gcatcttcga ccagagggga
     1381 ctaaagctga acagagaggc ggatgatgag agccccataa cccacttatc tttataccca
     1441 tgtggctgcc cttcctcaat atttttttct tcatattcca tactgccctt actcttttta
     1501 atatgagcgg ctgggctttt cgcaaaacaa gaaaatggca cctgcttacc attttactta
     1561 cagctgcttc ctggtttatc cttggcatct ggtacggctg gggttattgt gcctgcaccg
     1621 actggcactg gcaggtaaga gaagccatgg gcattaacga ccggtcgaat tcctatatcc
     1681 atttcctttt attgcagctt accgggaaag attttgacac caacctggtg gagaaggtga
     1741 cactgtatgt atttcttgct gcttcggtac tgagtatttg gcttaatctc cttgatcgca
     1801 gaaaagctgc ccggccccgg aaaattgtat agctccatag ttttatattt atagctaaat
     1861 cattcctcta tgcgccgctt ttttcttatg tctttgttgg cagcctcact tgttacttta
     1921 tccctgcata gtgttgcgca accttcaagg gcggaagaca gtattgccac catcatgcag
     1981 cagcatgaag tgatgggact ggctgtggct gtagtgaaga aaggaaaact ggtgtacact
     2041 cattccttcg gattaaagaa taaagaactg aaccagccat tgcaggagga gaacctcttc
     2101 cgcattgctt ctatttccaa atctttttct gctacagcta ttatgcaatt ggcagaagca
     2161 aaaaaattat cactggatga tgatgtaagc aacctcgtgg gctttacggt tcggcatccg
     2221 aagtttccca ataccatcat caccctgcgg atgatattat cgcaccgctc tgccatcaac
     2281 gacagccagg gctacttcac cctcgatgcc atcaacccgg ccaaaaatgc caactggcaa
     2341 aaatgctaca gtgagtatga accgggcacc ggctaccgct actgcaatct caattacaat
     2401 atggcgggta ccatcattga aaaaatatcc ggcgaacgtt ttgataagta tatacaaaaa
     2461 cacatcctcg aaccgctggg cctctacggc ggctattggg tagacgggct ggacagtacc
     2521 cgttttgcca gcatctatga gtatgatgat tcgctgaaga cgctggtggc acagccaatg
     2581 gcctatgcac cccgccggca ggagattggc agttatgtaa tggggtacag cactcccatc
     2641 ttctctccca ccggtggtat gaagatatcg gctccggacc tggcccgtta tatgattatg
     2701 cacatgaaaa tgggcaaggc caatggtaaa cggatcatta aaaagaaatc tgctatcacc
     2761 atgcaaacac ccttgtcaga aaaagaaggt tatggactgg ctattgctac tacggataaa
     2821 ctgatcgccg gcaaaacact aaaaggccat accggttcag cctatggctt gtacagcgcc
     2881 atgtacttcg accccaatga aaaatggggc atcgtggtca tcaccaatgg ctgccgccca
     2941 agttatacag aggggtataa tacggtgata cggaaaacgg tgaatgtcct ctacaaccat
     3001 atagtgagtg aaaaatagtg accgcattca ttccgtttca gcggaatcat ctgtgttcat
     3061 ctgtggccaa acacccattc cgctccagcg gaataatctg tgctaatctg tgttaatccg
     3121 tggccaaata cctattcacc gcaggtgaat tatcattctc tcttaattta ttcgtgacat
     3181 tagtgtcctg agcttgtcga aggattcgtg gctaaccaca ttccgcttca gcggaatctt
     3241 agtgtccttt gtgtcttcgt gggaaatccc tcttcgcttc agcgaaatct tctgtgtcct
     3301 ttgtgttttc gtgggaaaca catttcagcg gcagtcaaat cacccctgtt ggttattttg
     3361 tttggctggt ttcatgaaat gcaataacat tgacagatca atccaccccg caatccgcct
     3421 ccgctctgaa agcagcaaat ccttacctgc attagatcac tgtttaaaac caccgaatgc
     3481 gaaccagact gttgcaggca tctcttctgc tttttgcagg catcatcgtt tttaccagct
     3541 gtgtttccca aagaaagacg ggttgtgata ctaccggaaa gatcaaaagg gaaataaaat
     3601 tcaaaggctt tctttaataa tggcgggata tatatggtag caggcttcga caagctcagc
     3661 ctgacatgct gctttattta aacacttttc tcctttacta ccgggtaatt tgtagagatt
     3721 gtcaggctgt cccgatagca atcgggattg tcgaagcctg tatcaatgca cttttcttct
     3781 ttaccaccat gtaatattga aactgtgaac cactgtgaca ccccggttca acccaataat
     3841 taccgggctt accgcctgac acttatcatc gcggtaaata ctgcctatac attaccttta
     3901 accgaaacaa ttttgcttca tcgaataatc aataaccatg agccttgccc aacaattcat
     3961 tgctgaaata cagcaggaag ctgccgctac caaacgatta ctgtctattg ttccctggga
     4021 aaaaggagac tataagcccc acgaaaaatc catgacactg aaacgccttg catctcatgt
     4081 agccgagatc aatggctggt ggaaagaatg cctggtgcag gacgaactgg actttgccaa
     4141 agacagtggc gaaagaaaag aacatgccga caccggagcg atggtggcct ggcatgatga
     4201 actggtacag aaagcagtgg ctatacttca aaatactccc gatgaagaat ttgccaaacc
     4261 ctggaccatg cgggccggtg agcaaatctt ttttacactg cccaaaggag aagtggtgcg
     4321 cacctggtgc ctcaatcatt tgtatcatca ccgtggacag ctgaccgtct atctccgctt
     4381 gctggatatt ccgctgccgg gcacttacgg cccttcggca gatttccagg gataaacata
     4441 gctggcatca ctgctgaaaa tacactttgc tgatcttgcc tttttcaata aggtaaatgg
     4501 caacagcatg caaaggcttc gggccaaaac cggatacttc ttcatgatca atgaccatat
     4561 tgccctgcac taccctgttc tgcaggcggc agaaaagttt cggcactttg gtaatgaaga
     4621 gatagtcttt ccgcatctgc tcctttcctt ttgacaaaag ggtattggga aactggtaga
     4681 tctcaacatc ctctgcatac ggcgctaaaa aagcttcgag gttgtgggca ttataggcat
     4741 tcagttgctg ctgcaccaaa tcaacaggcg aaattgtaat caatgaatca ggataaaaca
     4801 tcgctcccct gttcatcacc cattctacct ttctccagtt gctcaaacta tccagcgggt
     4861 tggcggtgag caataacata ttggccaatt tgttttgggc gatacttccc cattcctttt
     4921 cctgtcccat agcctttgcg ccattaatgg tagacgcttc gagcagttgc catgtattta
     4981 gtcctgcagc ctgcatggct ttcagttctt caaaataaga agcggcatgc tgtgtgccgg
     5041 tattgccggc atcggtaccg gtagcaatga tgacgccgcc atcaattaac ttcttcaggt
     5101 taacggccat cacagaatct gtctgccgct gcttttgatg ctgcgccggg tttttcagga
     5161 aattgatgta catgccggca agggcagtat ccggcagcgg gtagtcaatg atggtgcctg
     5221 ccagttcagg atgggtgatc ttcagttcat ccgtcgtaaa atcataggta tcacccagca
     5281 ccttgccata gttgccggct accactaaag tggggcagag tactgttttc ttttccttca
     5341 gcagttttac aaaatcatcc ggcactattt catcatccac actgtgtacc aaaaaatcgg
     5401 cgccggcttc cacggccagt tgtgccgtaa tgcgttccgt agcatgcaca gccacccgtt
     5461 tattgttttt atgtgcttca tcaatagctg cttttaccag cgccagnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acttcaggaa cataagtggt taacaacgga
     5701 ccggtgatgc ggatcagcgg tgcatagttt tttgtagtaa aagaatcccg ctgccgcaaa
     5761 aaattatagg tagcgcctac atcaatcacc gaagtaatgc cgactgataa ataacggcgc
     5821 aaaaaatctt ccatatggtt atgcacccat tggatctctt ctgcataggg ccggta
//
LOCUS       DAORLS010000196         5841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7463_length_5841_cov_1.985655, whole genome shotgun sequence.
ACCESSION   DAORLS010000196 DAORLS010000000
VERSION     DAORLS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_5841_cov_1.985655"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PK977_13650"
     CDS             <1..905
                     /locus_tag="PK977_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRF19213.1"
                     /translation="IGLGGIHSVEAMAKVKAVVPETVFPHGYAVLNADDDLVYKMKYD
                     LKCNIALFSMDENNPRIKEHCADHGLACVFENGFITIMKGNWKIRVLAARDIPITYEG
                     KAVHNINNCLPAILSAYLFRDITIEDIRTGLQTFIPSEKLTPGRLNFFHFKNFTMLAD
                     FAHNPHGMRLLCDFVSKLDYPYKVGVISGTGDRRDEDIRELGEISAQHFDEIIIRCDK
                     NLRGRTADEIIGLLKEGIEKVNPTIPVMTIPNEDQALDYIYENHRPGSLYTIMCDVVA
                     RALDKIKELKVREETEGIKSQQPV"
     gene            971..1041
                     /locus_tag="PK977_13655"
     tRNA            971..1041
                     /locus_tag="PK977_13655"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1003..1005,aa:Gln,seq:ttg)
     gene            complement(1071..1934)
                     /gene="rfbD"
                     /locus_tag="PK977_13660"
     CDS             complement(1071..1934)
                     /gene="rfbD"
                     /locus_tag="PK977_13660"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRF19214.1"
                     /translation="MEAKPKILVTGANGQLGKELKQLESQYPQFEFIFLSREDLPIHH
                     FELVRNFFKGYHPQYLINCAAYTAVDRAETEKDLAFQVNGEAVGVLAAVCKEYGTKFL
                     HISTDYVFDGTASAPYKEDATTNPQSVYGASKLEGEKEALQFNSESIIIRTSWVYSEY
                     GRNFVKTMIKLMKEKDQLYVVNDQIGSPTYAADLADAIMHIISSAKWSPGIYHYSNEG
                     VISWFDFAVAIRDLTESNCKVTAIPTTSYPTPAKRPVYSVLDKSKIEETYGISLKDWK
                     DSLRDCLNRLK"
     gene            complement(1934..>2319)
                     /gene="rfbC"
                     /locus_tag="PK977_13665"
     CDS             complement(1934..>2319)
                     /gene="rfbC"
                     /locus_tag="PK977_13665"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRF19215.1"
                     /translation="LHYQLPPYAQSKLVRVLRGKILDVVVDIRKGSPTYGKSYSKVLS
                     AKNKRQLLIPKGFAHGFSVLSEKAEVLYKCDEFYNKQSEAGIFYNDPELNIDWRIPAE
                     YAVVSDKDKILPVFAECNNSFVFEG"
     assembly_gap    2320..2329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2330..2500)
                     /locus_tag="PK977_13670"
     CDS             complement(<2330..2500)
                     /locus_tag="PK977_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRF19216.1"
                     /translation="MPFIKTDFPGLLVFEPTVFEDSRGYFFESFNEKIFQHEGISTHW
                     VQDNQSSSTYGVI"
     gene            complement(2503..3792)
                     /locus_tag="PK977_13675"
     CDS             complement(2503..3792)
                     /locus_tag="PK977_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF19217.1"
                     /translation="MYQELIDKKAKLAVIGLGYVGLPIALEFARKVSVIGFDISSKRI
                     EMMKQGIDPSNELGKEAFEGCDIVFTDSLDVLREAKFLIVAVPTPVDEHNVPDLTLVL
                     RASETVGKVIKKGDYVVFESTVYPGCTEEDCLPVIEKLSGLKNVIDFKLGYSPERINP
                     GDKQHTLATIVKVVSGCDAESLEEIAKTYELVVKAGVHRASSIKVAEAAKIIENTQRD
                     LNIALMNELSVIFDKMNINTYEVLEAAGTKWNFLKFQPGLVGGHCIGVDPYYLTYKAK
                     ELGYHSEVILAGRNINDNMGKYVAKKVIQHIIKNNGDVKSAKVLVKGATFKEDVSDIR
                     NSKVSDVVNALKEFYVNVEVEDPHADSDELIHEYGFGLVDKVANDYDAVIIPVPHKQY
                     KELDENYFVSITKPHGLIADLKGIYRSKITNRPYWSL"
     gene            complement(3802..4785)
                     /locus_tag="PK977_13680"
     CDS             complement(3802..4785)
                     /locus_tag="PK977_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF19218.1"
                     /translation="MERKRVLITGAAGFLGSHLCDRFINEGYHVIGMDNLITGDLKNI
                     EHLFKLEQFEFYHHDVTKFIHVPGKLDYILHFASPASPIDYLKIPIQTLKVGAMGTHN
                     CLGLAKAKGARILVASTSEVYGDPLVHPQTEEYWGNVNPVGPRGVYDEAKRYMESITM
                     AYHTFHGVDTRIVRIFNTYGPRMRLNDGRALPAFIGQALRGEDLTVFGDGSQTRSFCY
                     VDDLIDGIYRLLLSDYIMPVNIGNPIEISLKDFAEEILALTGNKVKIVYKPLPVDDPK
                     QRQPDITKAKTILGWEPKVSRAEGLKITYDYFKSLPHEELTKQPKEFISLK"
     assembly_gap    4813..4849
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4850..>5841)
                     /locus_tag="PK977_13685"
     CDS             complement(<4850..>5841)
                     /locus_tag="PK977_13685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRF19219.1"
                     /translation="SADLKYVLGVASDLGKLLPADGYTVIVDKSTVPVGTAQKVHAAI
                     AANYKGEFDVVSNPEFLREGVAVEDFMKPDRVVIGTTSERARKLMGDLYAPFVRQGNP
                     VIYMDERSAELTKYAANSFLATKISFMNEIARLCEKLGADVDMVRRGIGSDDRIGKRF
                     LFPGIGYGGSCFPKDVQALVKSSSEVNYEFEILNAVMKVNEQQKLHLIPAINKYFNND
                     LKGKHFALWGLAFKPNTDDIREAPALYMIEELTKAGATVCAFDPEAMNNVRSTVGDKI
                     QYGQSQYDTLKNADALIIATEWNEFRTPDFGKIAASLKNKAIFDGRNLFDLSAI"
BASE COUNT         1606 a         1340 c         1078 g         1770 t
ORIGIN      
        1 atattggtct cggaggtata cattctgttg aggcaatggc aaaagtgaaa gcagtagttc
       61 cggaaaccgt cttcccccat ggatatgcgg tattaaatgc ggatgatgac ctggtatata
      121 aaatgaaata tgaccttaag tgcaatattg ccttatttag catggacgaa aataatccac
      181 gcatcaaaga gcattgcgct gatcatggcc ttgcctgtgt tttcgaaaat ggttttatca
      241 ccatcatgaa aggcaactgg aagataaggg tcctggcagc cagggatata ccgattacct
      301 acgaaggaaa agctgttcat aatattaata actgcctgcc ggcaatatta tccgcttacc
      361 ttttcagaga tattacgatt gaagatatcc ggaccgggct gcaaacattt atccccagtg
      421 aaaaactgac accgggaaga ttgaatttct tccatttcaa aaatttcaca atgctggccg
      481 actttgcaca caatccacat ggtatgcggc ttttgtgtga ctttgtcagc aaactggatt
      541 atccttacaa agtaggtgtc atcagtggca ctggcgaccg tcgtgatgaa gacatccggg
      601 aactgggcga aatatctgct cagcattttg atgagatcat tatccgttgc gataaaaatc
      661 tcaggggcag gactgccgat gagatcatag ggctgctaaa agaaggtata gaaaaagtaa
      721 acccaactat tccggtaatg acaatcccta acgaagacca ggctcttgat tatatatatg
      781 aaaatcatag acccggttca ttatatacca ttatgtgtga tgtggtggct agggccctgg
      841 acaagatcaa agagctgaaa gtgagggaag aaacagaagg cataaagtcc cagcaacccg
      901 tctaaaatct ttcaaattcc ttttgggaga aaaggctgat agccctattt ttgcactccc
      961 aaaacaggat tgagctatgg tgtaacggta gcactacaga ttttggttct gtctgtctag
     1021 gttcgaatcc tagtagctca acagaggtcc cggtcaaaag ccgggatttt tcattttaac
     1081 cggtttaaac agtcccttaa gctatctttc cagtctttta gggatatacc ataagtctcc
     1141 tcaattttcg atttatccag aaccgaatac acaggccttt tagcgggggt aggataagat
     1201 gtggtaggta tggcagtcac tttacaattg ctttcggtca aatcacggat tgcgacagca
     1261 aaatcaaacc agctgattac tccttcattg ctgtaatgat aaataccggg ggaccatttg
     1321 gccgatgaaa tgatatgcat tattgcatct gcaaggtctg ctgcataggt aggcgaaccg
     1381 atctgatcat tgactacata caactggtct ttctccttca tcagttttat cattgtcttc
     1441 acgaaattcc tcccatactc agaataaacc caggaagttc ttataataat agattcagaa
     1501 ttaaattgta atgcttcttt ctctccttcc agttttgaag caccatatac gctttgggga
     1561 tttgtagttg catcttcctt gtaaggcgct gaagcagtgc catcaaatac atagtcagta
     1621 gaaatatgca gaaatttcgt gccgtactcc ttacatacgg ccgccagtac tcccactgct
     1681 tcgccattta cctggaaggc aaggtccttt tccgtttctg ccctgtccac ggctgtatag
     1741 gcggcacaat tgatgagata ttggggatga tagcctttga aaaaattcct gaccagttca
     1801 aaatgatgaa tgggtaaatc ttctctggat agaaagataa attcaaactg agggtactgt
     1861 gactccagtt gttttagctc ttttcccaac tgaccgttgg ctcctgtaac caatattttt
     1921 ggttttgctt ccattatcct tcaaaaacaa aactgttatt acattctgca aatactggca
     1981 gtattttgtc tttatccgaa accactgcgt attcagccgg tattcgccag tcaatattca
     2041 gttcgggatc gttgtagaaa atgccagcct cactttgttt gttgtaaaac tcatcacatt
     2101 tatacagcac ttctgccttt tcacttaaca cagaaaatcc gtgggcaaac ccttttggta
     2161 tcagtaattg tcttttattt tttgcagata acactttact gtagctcttg ccataagttg
     2221 gtgaaccttt acggatatcc accaccacat caaggatttt gccacgaagc accctgacca
     2281 gtttactctg ggcataaggc ggcaactggt aatgcaggcn nnnnnnnnng attacaccat
     2341 aggtggaaga tgactggtta tcctgtaccc aatgggtgct gattccctcg tgctgaaata
     2401 ttttttcatt aaatgattca aaaaaatagc ccctgctgtc ttcgaaaaca gtcggttcaa
     2461 aaaccaataa acccggaaaa tcagttttaa taaatggcat agttataaac tccagtatgg
     2521 gcggttagta atttttgatc tgtaaattcc tttcaggtcg gcgatgagcc catgcggctt
     2581 tgtgatgctt acaaaatagt tttcatccag ctctttatat tgtttatgag gcaccggaat
     2641 aatgactgca tcatagtcgt tggccacttt gtccaccaac ccaaatccat actcgtggat
     2701 gagctcatca gaatcggcat gcggatcttc cacctccaca tttacataaa attctttcag
     2761 tgcattcacc acatcactta ctttggaatt ccggatgtca cttacatcct ctttaaaagt
     2821 agcgccttta accaatactt tggctgattt cacatcgccg ttattcttga ttatatgctg
     2881 aatcactttc ttggctacat atttccccat attatcattg atattccgac ctgccagtat
     2941 cacttctgaa tgataaccca gctctttagc tttatacgta agataataag gatcaacacc
     3001 gatacaatgc ccacctacaa ggcccggctg aaacttcaag aaattccatt tggtaccggc
     3061 agcctccaat acttcgtagg tattgatatt cattttatca aaaatcacag agagttcatt
     3121 catcagagca atgtttaggt ctctttgtgt attctcaatg atcttggctg cttctgccac
     3181 ttttatagac gaagccctgt gcacaccagc cttcactaca agttcatatg ttttggcaat
     3241 ttcttccagc gattcagcat cgcagcctga aactactttt acgatggttg ctaaagtatg
     3301 ttgcttatct cccgggttaa tcctttcagg tgaataaccc aatttaaaat caattacgtt
     3361 tttaagtcct gataattttt caatgaccgg caaacagtct tcctctgtac agccaggata
     3421 aactgttgat tcgaatacca cataatctcc tttcttaatc acttttccta ccgtttcaga
     3481 agccctgagt acaagagtca ggtccggtac attatgttcg tcaaccggtg taggaactgc
     3541 cacaattaaa aacttagcct ccctcagtac atccagtgaa tcggtgaaaa caatatcaca
     3601 tccttcaaaa gcttcttttc ccagttcatt gctaggatca ataccctgtt tcatcatttc
     3661 aatccgcttt gaactgatgt caaatcctat cacagatact ttgcgggcaa actccaatgc
     3721 aatcggaagt cctacataac ccaatcctat tacggccagt tttgctttct tgtcaataag
     3781 ttcctggtac atgattattt tttatttcag gcttataaat tctttgggtt gcttggtcag
     3841 ttcttcatgc ggaagtgact taaaataatc gtaagtgatt ttcaaacctt ctgcacggct
     3901 cacctttggc tcccatccca gtatggtttt cgcttttgtg atatctggct gacgctgttt
     3961 gggatcatcc accggcaatg gcttgtatac tatcttcact ttattgccag tgagtgccaa
     4021 aatttcttcc gcaaaatctt tcaatgaaat ctcaatagga tttccaatat ttaccggcat
     4081 tatgtaatca cttaagagca acctgtagat tccatcaatt agatcatcca cataacagaa
     4141 actacgggtt tgtgaaccat ccccaaaaac agtaaggtct tctcctctca acgcctggcc
     4201 aataaaagcc ggtaaggccc ttccatcatt cagacgcatt ctcgggccgt aggtattgaa
     4261 tattctgacg atccgggtat ccacgccatg aaaggtatga taggccatag tgatagactc
     4321 catataccgc ttcgcctcat catacacccc tctcggaccc acagggttta catttcccca
     4381 gtattcttcc gtttgcggat gcaccaaagg atctccgtat acttcactgg tagacgctac
     4441 caatattctt gcccccttcg ctttagctaa ccccaagcag ttatgtgttc ccattgcacc
     4501 cactttcagt gtctgaatag gaattttcag gtaatcgata gggctggccg gagaagcaaa
     4561 gtgcaaaata taatcaagtt tgcccgggac atggataaac tttgttacat catggtggta
     4621 aaactcaaat tgttccagtt tgaataaatg ctcgatattc ttcaaatctc cggtaatgag
     4681 gttatccatg ccaataacat gataaccttc atttatgaag cgatcacaaa gatgagatcc
     4741 caggaacccg gcagcaccgg ttattaatac cctttttctt tccatctgtt ttctaattta
     4801 ctgtctctct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tatggcagaa
     4861 agatcaaaca gatttcggcc gtcaaaaatg gctttatttt tcaggctggc ggcaattttg
     4921 ccaaagtcag gtgttctgaa ttcattccat tccgtagcaa tgatgagtgc atctgcattc
     4981 ttcaacgtat catattggct ctggccatac tgaattttat caccaactgt tgatctgaca
     5041 ttattcatcg cctccggatc gaatgcacaa actgttgcac ccgcctttgt caattcttca
     5101 atcatataca aagccggagc ttctcttata tcgtctgtat ttggcttgaa cgctaatccc
     5161 caaagtgcaa aatgtttgcc ttttaaatca ttattaaaat atttattgat cgccggaatc
     5221 aggtgcagct tttgctgttc gtttaccttc attacagcat tcagtatctc aaactcataa
     5281 ttcacttctg aagatgattt aactagtgcc tgtacatcct ttgggaaaca actgccgcca
     5341 taaccaattc cgggaaataa aaatcttttt ccaatcctgt catcactacc gataccacgg
     5401 cgcaccatat ctacatccgc ccccagcttt tcacatagac gggctatttc attcatgaaa
     5461 gatattttgg tcgccaggaa tgaattggct gcatatttgg tcagctctgc ggaacgttcg
     5521 tccatgtaaa tgacaggatt tccctgtctt acaaaaggag catacaaatc acccatcagc
     5581 ttcctagctc tttctgaagt agttccgata actaccctgt ctggtttcat aaaatcttca
     5641 actgctactc cctcccttag aaattccgga ttgctaacta catcaaattc gcctttataa
     5701 tttgctgcaa tggcagcatg tactttttga gctgttccaa ccggaacggt acttttatca
     5761 acgatcactg tatagccgtc tgccggcaac agtttgccaa gatcgctggc tacacccagt
     5821 acatatttta aatcggcact g
//
LOCUS       DAORLS010000197         5819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7498_length_5819_cov_2.200555, whole genome shotgun sequence.
ACCESSION   DAORLS010000197 DAORLS010000000
VERSION     DAORLS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7498_length_5819_cov_2.200555"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>645)
                     /locus_tag="PK977_13690"
     CDS             complement(<1..>645)
                     /locus_tag="PK977_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19220.1"
                     /translation="PGNKRKIPKAIKPDLEAAAITVSGQLLLLGSGSKSPQRNVGWFI
                     DPTSKNADSIRLDTFYQRLQLNGIKELNVEGATAIPGSFILASRGSKGYPKNHLIFTD
                     TRFWVKQSLAQITTILTSSGTDSSFFSGVSGLAYAKSDRLLLTVSTEDTRNSMDDGAI
                     GKSYLWIINNISSKKRWKAINPDKIIELESLDPGFKGQKIESVCITKETSDFLHL"
     assembly_gap    646..704
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(892..1404)
                     /gene="purE"
                     /locus_tag="PK977_13695"
     CDS             complement(892..1404)
                     /gene="purE"
                     /locus_tag="PK977_13695"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRF19221.1"
                     /translation="MNPLVGIIMGSDSDLNVMQAASDILKQFGIPHEVTVVSAHRTPH
                     RMIEYAQKAKERGMKVIIAGAGGAAHLPGMVASVTILPVIGVPIKSSNSIDGWDSVLS
                     ILQMPNGVPVGTVALNAAKNAGILAAEILAIADESISQKVADYKNSLNNEVLVKVEKL
                     KKEGWENKFD"
     gene            complement(1459..>1796)
                     /locus_tag="PK977_13700"
     CDS             complement(1459..>1796)
                     /locus_tag="PK977_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF19222.1"
                     /translation="KRLIRQQELNIQYQRMFFDNQKSLLNLSVINAFLEYEQQKKALA
                     LEEENILLAKENVNIVFQVYKLNSTTLIQLKEAEKSLQDAYTRLITARYNTKLAETEL
                     LRLKGDLVK"
     assembly_gap    1797..1830
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1831..2779)
                     /locus_tag="PK977_13705"
     CDS             complement(<1831..2779)
                     /locus_tag="PK977_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF19223.1"
                     /translation="MKRSLLFILCSITLQAGAQRLLTLEEAIATALKQNFDIQLSKND
                     SAVAALNYSYRNAAFLPRLNANAGTTWNNNKQTQEFSNNTKREGKVKTNNLNSSVSLN
                     WTLFDGLRMFATRDKAEELVKLGELGIKQQIINTVANVITTYYNIVRQKQQLKAIEEQ
                     ILLSQERVKLAQYKLDIGVGAKPDVLQSKVDNNAQKALQLEQLTLISQLKEQLNQAMN
                     VLDAPPYDVSDSIPLNTKLSLGDIQNGLESTNPGLLITRKNIDIAGLTLKELKADRFP
                     VVSFNSAYNFSRNNNNLALNPALPVFSQNKGFNYGLTATI"
     gene            complement(2795..4672)
                     /locus_tag="PK977_13710"
     CDS             complement(2795..4672)
                     /locus_tag="PK977_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF19224.1"
                     /translation="MEKGMNKWQAAVEGSKEIYFAVIATSITLAVVFLPIIFLQGFVG
                     RLFREFGIVVAGAVLISAFVSLTLTPVLNVKLTKKDVHKHSWFYLKTEPFFRWMENTY
                     QKALRSFMKVRRLAFVLVGLCIAAIFFIGRNLQSELAPMEDRSQFRLQLTAPEGTSFD
                     AMDRFVNQLTNFMLDSVPEKDMVLSVTSPGFTGSGNANTGFVRVTLTPPNERKRSQKE
                     IVAMVNRQLPKYNEGRAFAIEEQTISVNRRGGQPVSFVIQNNNFDKLTAILPKILEEA
                     RQSKVLMNADVDLKFNKPELRVEIDRLKAAQLGVSVNDISQTLQLAYSNRRLGYFTKD
                     GKQYQVMGQVARSDRDDPNDLKMLFVRNSRGEMISLDNLVTTYESNTPPTIYHFNRYK
                     SATISAGLQPGFTIGDGIEEMNKIADKLLDETFTTSLSGSSRDFAESSSNTSFAFLLA
                     LGLIFLILAAQFESFIDPLIIMFTVPLAIAGAVLSLWLFGHTLNIFSQIGMIMLIGLV
                     TKNGILIVEFANQNRMKGMEKREAAIYSATQRFRPILMTSLAMALGALPLALSLGAAA
                     TSRIPLGVVIVGGILFSLVLTLFVIPAMYSFMSGKKKKSEIDQFIHKETKVPDTVTEH
                     S"
     gene            complement(4657..>5512)
                     /locus_tag="PK977_13715"
     CDS             complement(4657..>5512)
                     /locus_tag="PK977_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF19225.1"
                     /translation="ASSDAILSMTVQSNTRNQLQMTEYANNVLVERLQTIPGVSGVQI
                     WGEKRYAMRIWIDPAKLVALNVTAADVQAALLRENVELPSGKISGNTTELTVRTFGRL
                     NTEEEFNNLIVRNVGGADIRLKDVGEAVLGPENEETVLRGNGTPMIALAVVPQPGSNY
                     VSISDEFFRRLEQIKKDVPEDIKINIALDQTKFIKKSILEVEETLMIAFLLVVMIIYA
                     FFREWIIAIRPLIDIPVSLIGAFFIMYLMGFTINVLSLLAIVLATGLVVDDGIVVTEN
                     IFKRWRRE"
     assembly_gap    5513..5547
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5548..>5819)
                     /locus_tag="PK977_13720"
     CDS             complement(<5548..>5819)
                     /locus_tag="PK977_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF19226.1"
                     /translation="LGVRDYPAIDPPNISVRTSYPGANSDIIETQITEPLEKAINGIA
                     GIKNITSSSSNGSSNINVEFDLSIDLEAAANDVRDKVSQAQRSLPS"
BASE COUNT         1525 a         1236 c         1215 g         1715 t
ORIGIN      
        1 aaggtgcaga aagtcggatg tttcttttgt aatacaaacg gattcaattt tttgtccttt
       61 aaatccggga tccagtgatt ccagttcaat gatcttgtca gggttgatgg ccttccatct
      121 ttttttactg cttatattat ttatgatcca gagataactt ttgccaatag ccccatcgtc
      181 catactatta cgggtatctt cggtagaaac tgtcagaagg agccggtcgc tttttgcata
      241 agcaaggccg gaaacaccgc tgaaaaagct gctgtctgtt ccggaacttg tgagtatagt
      301 ggtgatttga gccagtgatt gttttaccca aaatctggta tcggtaaaga ttagatggtt
      361 ctttggataa cctttgcttc ctcttgaagc aagaataaaa gagccgggaa tggctgttgc
      421 accttcaacg ttgagttcct tgatgccatt cagttgtaat cgctgataaa aagtgtcaag
      481 acgaatgctg tcggcatttt ttgaagtggg atcaataaac caccctacat ttcgttgtgg
      541 tgatttagaa cctgaaccta ataacaacaa ctggccgcta accgttatgg ctgccgcttc
      601 caaatccggc ttaatcgctt taggaatttt tcttttatta ccgggnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgagt acatgggtag
      721 catcgtcacc aataacaaag aattttttat tttgatattc tattccggat gctgaaggat
      781 aggaggggaa ttcaacaact tcttcgagta attcattagt gccacaagaa gttatgaaaa
      841 gtagcaaacc gattaaggaa aaacggaaat acatgctggc taacagaaag gttaatcaaa
      901 tttattctcc catccttctt ttttcagttt ctccactttt accaacactt cattgttcag
      961 cgaattctta tagtctgcta ctttttgtga aatgctttcg tccgcaatag caagaatttc
     1021 tgctgcaagg atacctgcgt ttttagccgc attcagtgca acagtgccca ccggaacacc
     1081 attgggcatt tgcagtatag acaaaacaga atcccaccca tctattgaat tggaagattt
     1141 gatgggaaca ccaattaccg gcagtatggt aacggacgcc accatgcccg gaagatgcgc
     1201 tgctccacca gcacctgcaa taatgacttt catgcctctt tcctttgcct tttgagcata
     1261 ctcgatcatg cggtgtggag ttcgatgagc tgatacgact gttacttcgt gggggatgcc
     1321 aaattgtttc aggatgtctg aggcagcctg cattacattc aggtcgctat cgctgcccat
     1381 gatgatgcca accaatggat tcatgcggat aaatttgatt tcaaaagtaa cccgaaacaa
     1441 tcaaccagca accagaatct atttcacgag gtcacctttt aatcgcaaca attcagtttc
     1501 tgccagtttc gtattataac gggccgttat cagccttgta taagcatcct gtaagctttt
     1561 ttcagcctct ttcaattgaa ttagagtggt agagttcagt ttatagacct ggaaaacaat
     1621 attgacattt tcttttgcca ataaaatgtt ttcttcttcc aaagccaatg cttttttctg
     1681 ttgttcatat tccaggaagg cattgatcac agaaagattg agtaacgact tctggttgtc
     1741 aaaaaacatc cgctggtatt ggatgttcag ttcctgctgc ctgataagcc gtttggnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtattgtagc agtgagacca taattgaaac
     1861 ctttgttttg gctgaaaacc ggcagggcag ggttcagagc aaggttatta ttgttcctcg
     1921 aaaagttata ggctgaattg aatgaaacca ccgggaacct gtcagctttt aattctttta
     1981 gcgtaagacc tgctatgtca atattcttcc gtgtaatcaa taagccgggg tttgtggatt
     2041 caagtccgtt ctgaatgtcg cctaatgaaa gttttgtatt caagggaata gagtcactaa
     2101 catcatatgg aggggcatcc agcacattca tagcctggtt aagttgttct ttcagctggc
     2161 taattaaagt aagctgctcc agttgcaatg ctttttgtgc attattgtcc actttacttt
     2221 gcagcacatc aggcttggcg ccaacaccaa tgtccagttt gtattgcgcc agttttactc
     2281 tttcctggct cagaaggatc tgttcttcaa tcgccttcag ttgttgtttt tgtctgacta
     2341 tgttatagta agtagtaatt acattggcca cggtattaat aatctgctgc ttgatgccca
     2401 gttcgcccag ttttaccagt tcttctgcct tgtctcttgt ggcaaacatt cgcagtccgt
     2461 caaataatgt ccagttaaga cttacactgc tgttcaggtt atttgttttt actttacctt
     2521 ctcttttggt attgttgctg aactcctgag tctgtttgtt attgttccat gttgtaccag
     2581 cattggcatt cagtcgtggt aagaaagctg cgttacgata tgaataatta agtgcagcta
     2641 ctgcagaatc attttttgac aattgtatat caaaattctg tttcaatgca gtggcaatgg
     2701 cttcttcgag agtaagcaaa cgctgtgcac cggcttgcag agttatggaa cagagtataa
     2761 aaagcaaact tcttttcata atgatcttgt cgtcttaaga atgttctgta acggtatcgg
     2821 gtacttttgt ttccttgtga ataaattggt caatctcact cttcttcttt ttacctgaca
     2881 taaaggagta catagctgga attacgaata aagtaagcac cagtgaaaat aagatgccgc
     2941 caacaatcac cacaccaagc ggaatacggc tggtggcagc ggcgcccaat gaaagggcaa
     3001 gaggtaatgc acccagtgcc attgcaaggc tggtcatcag gataggacga aaacgttgtg
     3061 tggcagagta aatagctgct tctcttttct ccatgccctt catccggttt tgattggcaa
     3121 attctacgat aaggattcca tttttggtca caagaccaat tagcataatc ataccgatct
     3181 gtgaaaagat attcaaagtg tgaccaaata accacaatga aagaacagct cctgcaatgg
     3241 ccagcggtac cgtaaacatg atgatcagcg gatcaataaa actttcaaac tgagcagcca
     3301 ggatcaggaa tatcagtccg agggccagca ggaaggcgaa gctggtattg ctggaacttt
     3361 cagcaaaatc tcttgatgaa cctgaaagag aagtggtgaa ggtttcatcc agtaatttat
     3421 cagctatctt attcatttct tcaataccgt ctccaatagt aaacccaggc tgaaggcctg
     3481 cagagattgt ggctgattta taacggttga agtgatagat cgtaggcggg gtgttggatt
     3541 cataagtggt caccaggtta tccaggctga tcatttcacc ccggctgttt cgcacaaaga
     3601 gcatcttaag atcgttaggg tcatccctgt cgctcctggc tacctggccc attacctggt
     3661 actgtttacc gtctttcgta aaatacccca ggcggcggtt actgtaggcc agttgtaatg
     3721 tttgtgaaat atcgttgaca cttacaccga gctgtgctgc cttcagcctg tcaatttcaa
     3781 cccttaattc cggcttattg aacttgaggt caacatcggc attcatcagt actttacttt
     3841 gcctggcctc ttccagtatt ttaggaagta tggcggtcag tttatcgaag ttgttattct
     3901 ggataacaaa cgaaacgggt tgtccgccac ggcggtttac agagatggtt tgttcttcga
     3961 tggcaaaagc tcttccttcg ttgtatttgg gaagttgacg gtttaccatg gccacaattt
     4021 ccttctgcga cctttttctt tcgttgggtg gtgttagcgt cactctcaca aaaccggtat
     4081 tggcatttcc tgagccggta aagccgggag aggtaacgct cagtaccata tccttttccg
     4141 gcacagagtc gagcatgaaa ttggtgagct ggtttacaaa ccggtccatg gcatcaaacg
     4201 aagttccttc cggtgccgtt aactgaagcc tgaactggct tctgtcttcc attggggcta
     4261 actctgactg aagattgcgg ccgatgaaaa agatagcagc gatacacaag ccaactagca
     4321 caaaagccaa tcttcttact ttcataaacg acctcagggc tttttgatag gtattctcca
     4381 tccagcggaa gaacggttct gtctttaaat aaaaccagga atgtttatga acatctttct
     4441 tggtaagctt tacgttcaat accggtgtca aagtcaggga tacaaatgcg gagataagta
     4501 cagcgccggc taccacaatg ccaaattcac ggaaaagcct tccgacaaat ccctgtaaga
     4561 aaatgatcgg cagaaataca actgccaaag tgatagaagt ggcaataaca gcgaaataaa
     4621 tttctttcga accttcaact gcggcctgcc atttgttcat tcccttctcc atcttttgaa
     4681 gatgttttct gtaacaacga ttccatcatc gaccacaagg ccggtagcca gaacaatggc
     4741 cagtaatgaa agcacattga tggtaaaacc catcaggtac atgatgaaaa aagcgccaat
     4801 gagtgataca gggatgtcaa tcagcggacg gatagcaatg atccactccc ggaagaaagc
     4861 atagatgatc atcaccacca gcaagaaggc aatcatcagc gtttcttcta cttccaggat
     4921 tgacttcttg ataaatttgg tttggtccag tgcaatattg atcttgatat cctcaggaac
     4981 atctttcttt atctgctcta atcttctgaa aaattcatcg gagatagaaa cgtagttgga
     5041 accaggttgc ggtacaactg ccagcgcaat catgggagta ccattacccc tgagtacagt
     5101 ttcttcattt tcgggtccca gcacagcttc acctacatct ttcagcctga tatccgcacc
     5161 gccaacattt cttacaataa gattattgaa ttcttcttcc gtattcagac ggccgaatgt
     5221 tcttacagtc agttcagtag tgttgcctga aattttacct gatggcaatt caacattttc
     5281 tcttagtaaa gctgcttgta catcagctgc tgttacattc agggctacca gttttgcagg
     5341 gtcgatccag atgcgcatcg catatctttt ttcaccccag atctgcactc cacttacacc
     5401 gggaattgtc tgtagccgtt ctaccaatac attattggca tattcggtca tttgcaactg
     5461 gttccgggtg ttgctttgta cggtcatgga aagaatagca tcggaactag cannnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnncag agggcaaaga tcgctgcgcc tgggaaactt
     5581 tatcccgcac atcattggca gctgcttcca ggtcaatact caggtcgaat tcaacattga
     5641 tattactgct gccattgctg ctgctggaag taatgttttt gatacctgca attccattaa
     5701 tagctttttc cagcggttct gtgatctgtg tttcaataat atcagaattg gcgcccgggt
     5761 aagaggtcct tacactgata ttgggcgggt caatggcagg ataatccctg actcctaaa
//
LOCUS       DAORLS010000198         5819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7500_length_5819_cov_1.605656, whole genome shotgun sequence.
ACCESSION   DAORLS010000198 DAORLS010000000
VERSION     DAORLS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7500_length_5819_cov_1.605656"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1517
                     /locus_tag="PK977_13725"
     CDS             237..1517
                     /locus_tag="PK977_13725"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF19227.1"
                     /translation="MKVIIAGAGFGGLRLARQLSNKPGFEIVLIDRFNYHQFQPLFYQ
                     VATAGLDASNISFPLRKVFQKSKNVRIRLASLKAVQPGENKVITDSEEIFYDVLVLAT
                     GADTNFFGNQQLANHAFPMKSTVEALQIRHRLLQLFENAHTIKDESTLQRLMNIVVVG
                     GGPTGVEVSGALAEMKKYVLPKDFPELDFSRMNIYLLEGSPKTLASMSEKASAKSAGY
                     LNKLGVKLLTNTLVKDYDGKNVVLQDDKNIASSTVIWAAGIKGNIPNGIDISLIARGN
                     RIKVDRQCRVQGHSNIYALGDLAYMETDGYPNGHPQVAPVAIQQADLIAGNLRLIEKK
                     ANPERLYQFSYRDKGSMATVGRNLAVVDVPKPRLHLSGFFAWLIWMSLHLMLILGVKN
                     RFFVFINWLYNYVTYDQNLRLIFKEFNRKEPEKK"
     assembly_gap    1613..1768
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1769..1966
                     /locus_tag="PK977_13730"
     CDS             <1769..1966
                     /locus_tag="PK977_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19228.1"
                     /translation="QTGLAVALTGLIAAYWTGKVVTQFAYYPMYQIPRKAIFKMGEVL
                     MNILFISFAVIFILLFIQNLS"
     gene            complement(1985..2548)
                     /locus_tag="PK977_13735"
     CDS             complement(1985..2548)
                     /locus_tag="PK977_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19229.1"
                     /translation="MKKCFAFLIFIPVLILIASCRDANTKTETTDTNNVMNNQTDSTG
                     RSSVTNVHDVVNEKKSTAPGNIVVREMPNEILANIDQHLISKVSFSDPPVGGGINNGI
                     VTIRNTLPDVTFQKVIVEVSILTESNEEYRADYYTFQNVEPGDSKSAKMPKTTRGIKA
                     ICHVIKVKSNELTNGEMILVGDKFSPR"
     gene            complement(2757..2993)
                     /locus_tag="PK977_13740"
     CDS             complement(2757..2993)
                     /locus_tag="PK977_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19230.1"
                     /translation="MDNPQVAALIYLQMKNEILLKVFFQQYLGHAEYSRAIAALYEAL
                     PSSLESAFLDNPQLMDFRDAVMKIYHYEKNPMED"
     assembly_gap    3267..3281
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3381..>3807)
                     /locus_tag="PK977_13745"
     CDS             complement(3381..>3807)
                     /locus_tag="PK977_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19231.1"
                     /translation="IKRKNRIRLTVSSLSLWLSNITHIMSKSLALCSEGGNFAKHTDK
                     RSGGCVSKENTGCEPTANSSEPKAKEKHSLKEWEIIKTDKKLKWVKLGASTEIHSVPS
                     LFVNSILVRRPDLNAVVSALSQRHQLLSKRGSIFPQNPS"
     assembly_gap    3808..3847
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3993..>4300)
                     /locus_tag="PK977_13750"
     CDS             complement(3993..>4300)
                     /locus_tag="PK977_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19232.1"
                     /translation="AKRDMLDKKYGDDLDQAREPYEKAGAILADRASKEGGLELREKE
                     QYKKVANYLSEIYGYKRVQAKSKPADQAKFQAEEKKWNEIYDSINAIKTLKKGHDAN"
     assembly_gap    4301..4349
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4350..5378)
                     /locus_tag="PK977_13755"
     CDS             complement(<4350..5378)
                     /locus_tag="PK977_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19233.1"
                     /translation="MKKLLVVSVVCLSGLVTTAQNYDNIKNQLLFNKFEQAKTDFDKA
                     FTNAKFASKAEAFILKTAIYAGLAMSDANKDKPVGMQLANEADAAFKKYREMDPEMKL
                     VKDPVYQNGPVNLYSAFYSGGYEDYKTKSWQAGYDKLKKAVELSDILIAQKIFNSPLD
                     TNVLILAAITAESSDNKEDAAKYYVRLADAKVDGDGFESVYRFLVSYYFGKKDIPNFE
                     KYKTLGAQLYPKSEYFTFDKVDFAAGLATNFDDKVKAVEEILASDPDNFKANQVLGEI
                     IYDTLNSEKEGAVLPANAADLEKRMVASFLKAASVRANYEIPFLYMGDHFINKAAKTN
                     DARSAHAAD"
     gene            complement(5405..>5819)
                     /locus_tag="PK977_13760"
     CDS             complement(5405..>5819)
                     /locus_tag="PK977_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRF19234.1"
                     /translation="CVNEEDKANRTVLVVSEQGFGKRTPVDEYRFTNRGGKGVKTINV
                     TEKTGSLVGILDVAENEDLMITCKSGVTIRMPVSGISEQGRATQGVKLIRLDDGDEIA
                     AITQLDDQGQNGNGDHGDADATSSDENPSTDGNEA"
BASE COUNT         1575 a         1139 c         1076 g         1769 t
ORIGIN      
        1 gacaatacct aaaaacaaat gatgaaatcg gttatgtggg tctggatgta gacaaaaata
       61 tatctgcgca tgccctgcac ctgctaaggg aggtaaaaca tactattaaa gtaaggctat
      121 tatattgacc ggcgtacttc tttgaaaagc ttgagcgata ttttctaaat aaatattcct
      181 atattttcaa taattattga aaataatgta aatttgtgat gtaaaatcgt acgatcatga
      241 aagtaattat cgccggagct ggttttggag gtttacgact ggcccgccag ctcagcaata
      301 aacctggttt tgaaatagtg ttgatagaca ggttcaatta tcaccagttt caaccgcttt
      361 tttaccaggt ggcaacggca gggctggatg ccagtaatat ttcattccct ttacgtaaag
      421 tgtttcagaa gagtaaaaat gtacgtatca ggctggccag tctgaaagcg gtgcaaccag
      481 gagaaaataa ggttattact gattcagagg agatttttta cgatgtgctg gtattggcta
      541 ccggagcaga cactaatttc tttggtaacc agcaactggc gaaccatgca tttcccatga
      601 aatccacggt ggaggctttg cagatcaggc acagattatt gcaacttttt gagaatgctc
      661 atacaatcaa agatgaatct acgctgcaac gattgatgaa tattgtagta gtgggaggtg
      721 gaccaaccgg ggtggaagtg tcaggcgccc ttgcagaaat gaaaaaatat gtgctgccga
      781 aagattttcc ggagcttgat ttttcccgga tgaatattta cctgcttgaa ggatccccaa
      841 agacccttgc ttccatgagt gagaaagctt cagcgaaatc ggccggttac ctcaataaac
      901 taggggtaaa actactcact aataccttag taaaagatta tgacggaaaa aatgtcgttt
      961 tgcaggatga caaaaacatt gcctcatcaa cggtaatttg ggcagccggc attaaaggta
     1021 atattcccaa tggcattgat atttctctga tagcgagagg aaacagaatt aaagtagaca
     1081 ggcaatgcag ggtacagggt cattcaaata tttatgcctt aggtgatctt gcttacatgg
     1141 aaactgatgg ctatcccaat ggccatcctc aagtggcgcc agtggccatt caacaagcag
     1201 acctgatagc cggtaatcta cggcttattg aaaagaaagc aaacccagag agattgtacc
     1261 agttttccta ccgtgacaaa ggatcaatgg caacagttgg gcgaaacctg gcagtagtag
     1321 acgttccaaa accccggcta caccttagtg gcttttttgc ctggttgatc tggatgagcc
     1381 tgcacttaat gctgatactt ggtgtaaaga acaggttttt tgtatttatc aactggcttt
     1441 ataattatgt gacttatgac cagaacctgc gactgatctt taaggaattc aacagaaaag
     1501 aaccagaaaa aaaatagatc atgaacctca ccattcccaa ctggctaatc atactcggcg
     1561 gtgccgggca aatttttaca gccattattt atccctatat acggcatgtg gtnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca aactggattg gcagttgccc ttacaggttt
     1801 gattgccgca tactggacag gaaaggtcgt aacacaattc gcctattatc ccatgtatca
     1861 aataccgaga aaagcaatct ttaagatggg tgaggtgcta atgaacatct tatttatatc
     1921 atttgccgtt attttcatac tgttgtttat tcaaaacctc agttagtgaa ggtataaaga
     1981 ttccttacct gggtgagaat ttatcgccta caaggatcat ttcaccgttg gtcaactcat
     2041 tgctcttaac ttttattaca tggcatatag ccttaatacc ccgggtggtc tttggcattt
     2101 tagctgattt gctgtcacca ggttctacat tttgaaatgt gtagtaatca gcccggtatt
     2161 cttcatttga ttcagtcagg atgcttactt ccacaatgac tttttgaaaa gtgacatcag
     2221 gcagggtatt cctgatggtt acgattccgt tattaatgcc gccaccgact ggcggatcgg
     2281 aaaaactaac cttactgatc aggtgctgat caatgttagc aagaatttcg ttaggcattt
     2341 ctctgacaac gatattgccg ggtgctgttg attttttttc gtttaccaca tcatgtacgt
     2401 ttgtaactga actccgtcca gttgaatcag tttgattatt cattacgtta ttggtatcag
     2461 tggtttcagt cttagtgttc gcatctcgac aagatgcgat taaaataagc acgggaataa
     2521 atattaagaa tgcaaaacac tttttcatag aaggtcattt atagttcaag ctacttgata
     2581 tcagtctttt ggtcaatacc caatatccac aaaaaatact tacaattaat tcctataatg
     2641 tttactacgt tatgttcaat aggttagctg caccttatgt ttttattcaa aggtgcaaga
     2701 gcatagatgt ggtgggctaa aaaaataatt tatggactgt aatgactctt caaatgttaa
     2761 tcctccatcg ggttcttctc gtaatgataa atcttcatca cagcatcacg aaaatccatc
     2821 agttgaggat tgtctaagaa agcactttca aggctggagg gaagagcttc gtacagagct
     2881 gcaatggctc gtgaatattc agcatgccca agatattgtt gaaaaaagac ctttaacaga
     2941 atttcatttt tcatttgaag ataaatgagt gctgcaactt gaggattatc cataaattat
     3001 ttttttgaag gtgggctgtg cgcctattta cacataacgt atattataag aaaacttcct
     3061 tgcccctgta agttgcgaat ttttacttct atatccaaaa attggagatt ttaaaaattc
     3121 gcaacttaca ggggccgtcc tcatcattgt ttcttttctt ataatttaca ttatgttaag
     3181 tcgctttctc aaaagaaaat acggggtgtg gcgtgagaac cagagagact cctattttat
     3241 aggtttgcgt atcttatacc aaaactnnnn nnnnnnnnnn natgaaagaa aaattaatat
     3301 ttatgatgct gccatattcc gcattcaggc aatgcaagag caaacactaa gattgcattg
     3361 tgaaatgtat gcccatatag ctaagaagga ttttgaggaa atatactccc tcgtttggaa
     3421 agaagttgat gcctctgtga aagagctgat acaactgcat tcaaatctgg gcggcgaacc
     3481 agaatcgaat taacaaataa tgaaggaact gaatgaattt ctgttgaagc acccaatttg
     3541 acccacttca actttttatc agtcttgatg atttcccatt cttttaatga atgtttttcc
     3601 ttcgccttcg gctcagatga gttcgccgtt ggctcacacc ccgtattttc ttttgagaca
     3661 catcccccac ttcgcttgtc ggtatgcttt gcaaaattac cgccttcgct acacagcgcc
     3721 aacgacttag acataatatg agttatattc gaaagccaca gagacaagga agatacggtt
     3781 agccttatac gattttttct tttaatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnccg tataaaattt attgtggctt taaatataac agcccattat gttaaattaa
     3901 tattgataac aataccacca ctatatccac tgcagacata aacaaagaac ccggccattt
     3961 agccgggttc tttgttttct ttgaaataaa acttaattgg catcatgccc tttcttcagg
     4021 gtcttgattg cattgatgct gtcataaatc tcgttccatt tcttctcttc agcctggaat
     4081 tttgcctggt cagcaggctt acttttggcc tgaactcttt tatagccata tatttctgaa
     4141 aggtaattcg caactttctt gtattgctct ttttctctta attccaatcc tccttcttta
     4201 gaggctctgt cagccaggat agcaccggct ttttcatatg gttctcttgc ctggtcaaga
     4261 tcatcgccgt attttttatc aagcatatct cttttggcta nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcagcagcat gggcagaacg ggcatcgttg
     4381 gtcttagcag ctttattgat aaaatgatcc cccatatata agaaaggaat ctcgtaattg
     4441 gcccttacag aggccgcttt caggaatgaa gccaccattc ttttttcaag gtcagcagca
     4501 ttggcaggaa gtacggctcc ttctttctct gagttcagtg tatcataaat gatctcaccc
     4561 aatacctggt tagccttaaa attatcgggg tctgaagcca atatttcttc aacagctttt
     4621 actttgtcat caaaattggt ggcaaggccc gcagcaaaat ctactttatc aaaagtgaaa
     4681 tactcacttt tgggatacaa ctgagctcct aaggttttat atttttcaaa attgggaata
     4741 tccttttttc caaaatagta actgacaagg aagcgatata cgctttcgaa accgtccccg
     4801 tcaactttag catctgctaa acgaacataa tattttgcgg catcttcttt attatcgctg
     4861 ctttctgctg taatagctgc aagaatcaat acattagtat ccagtggtga attaaaaatc
     4921 ttctgtgcaa taagtatatc tgataattcc accgcttttt tcaatttgtc gtaaccggcc
     4981 tgccatgact ttgttttata atcttcataa ccgccggaat aaaaggctga atacaggtta
     5041 actgggccat tttggtacac cggatctttc accaatttca tctcgggatc catttccctg
     5101 tattttttaa aggcagcatc agcctcgtta gccaattgca tgccgacggg tttgtcttta
     5161 ttggcatcgc tcattgccag gccggcatat attgctgttt tgagaataaa ggcctctgct
     5221 ttactggcaa attttgcgtt ggtaaaagct ttatcgaaat ctgttttggc ctgttcaaac
     5281 ttgttgaaca aaagctggtt cttgatattg tcataattct gggctgttgt tactaaccct
     5341 gacaagcaaa ccacagatac aaccaggagt tttttcattt tgataattat tgatttaaag
     5401 aattttatgc ttcatttcca tctgtagatg gattttcatc tgatgacgtt gcgtcagcat
     5461 ctccatgatc gccattgccg ttttgcccct ggtcatccag ttgtgtgatg gctgctattt
     5521 catcaccgtc atccagcctt atcagtttta ccccttgtgt agccctgccc tgctcactga
     5581 taccactaac cggcattcga atggtaacgc cacttttaca ggttatcatc aggtcttcat
     5641 tttctgctac atccagtata ccgacaaggc ttcctgtttt ttctgttaca ttgatcgttt
     5701 tcaccccttt tcctccccgg ttagtaaaac gatactcatc taccggtgta cgtttaccaa
     5761 atccctgttc acttactacc agcacggttc tgtttgcttt gtcttcctca tttacacaa
//
LOCUS       DAORLS010000199         5799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7538_length_5799_cov_1.974930, whole genome shotgun sequence.
ACCESSION   DAORLS010000199 DAORLS010000000
VERSION     DAORLS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7538_length_5799_cov_1.974930"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>251)
                     /locus_tag="PK977_13765"
     CDS             complement(<1..>251)
                     /locus_tag="PK977_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HRF19235.1"
                     /translation="PATDIYFSTGPMDVLDHSCSKMGFGGKMCIDGTAKMIEETDDNY
                     QSASTSEQLPDGFLESVFSEIKSVNTALLKKQIPCLIVSV"
     assembly_gap    252..281
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<282..>611)
                     /locus_tag="PK977_13770"
     CDS             complement(<282..>611)
                     /locus_tag="PK977_13770"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRF19236.1"
                     /translation="TAITHKKSPVYPATIVGMPPQEDAWLGKATERIFLAPIKMTMVP
                     EIMDMDMPVEGVFHNLVITKINKEYAGQGQKVMNAMWGAGQMMFNKILVLADEKVRIQ
                     DYESLAKY"
     assembly_gap    612..666
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<667..1527)
                     /locus_tag="PK977_13775"
     CDS             complement(<667..1527)
                     /locus_tag="PK977_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRF19237.1"
                     /translation="MAWKNQQQFIEALEKAGELVRIQTYVNPKLEIAEVTDRISKSGG
                     GGKALLFENTGYDFPVLMNAYGSEKRMCMALGVNHLDDVAREIEELFKMLAAPKEGIL
                     DKLKMLPKLGQFASWMPKVKSGRGECQEVIMGNPDITKLPVITCWPKDGGPFVTLPVI
                     HTKDPNTNSRNVGMYRMQVFGPRLTGMHWHKHKVSAKHFSEYKKLNKKMPVAVALGGD
                     PVYAYSATAPLPENVDEYMLAGFLRKKKVELVKCISQPDIEVPTDADFIIEGYVDPTD
                     ELIWEGPFGDH"
     gene            1677..3722
                     /locus_tag="PK977_13780"
     CDS             1677..3722
                     /locus_tag="PK977_13780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRF19238.1"
                     /translation="MMRSKYFLLALVSGALLFVSCKTSSKKEDSKPDVLAANLDTTIN
                     PADDFFMYANGGWIKKNPIPGDQSSWGIGNLVIEENLKRLREISEKAEKAKAVKGTPE
                     QKIGDFWTTAMDSAKIEADGLKPLKPFLDKINAITDIKSLVNTVADLKKAGSSTLFSD
                     FVTQDDKNSEIMTYKLWQGGIGLPEREYYFKTDSTTVNIRNAYVKYITDILTMSGEDA
                     VAAQIAAKNILALETKLAQASRKLEDLRDPYKNYNKMAVTDLGKMNASVDWSSYLSVT
                     GIKNLDSVIVGQPEFFKALDNALKTVPLADWKSYVKFNLITDFAGALPDQYGIAAFNF
                     SKLFSGAKERKPRWKRVISSEESVMGEMLGQLYVKEFFNEKAKKRYEDLVEAIRDALK
                     ERIGKLTWMSDSTKQKAYVKLAAIKKKVGYPDKWKDFSSMEIGRESYVQNLVNANNWW
                     HNYEINKLGKPVDRDEWGMYPQTYNAYYNPSNNEIVLPAGIFTVPGYRDEELDDATVY
                     GYAGASTIGHEITHGFDDEGRQFDAKGNLVSWWTTKDEEEFKKRAEVMIQQFNEYEPV
                     KDYHINGKATLGENIADLGGILLGIEAFKKTKQYQENKTLGGLTPMQRYFCGYALGWL
                     GHTREENLKNQLLTDVHSPAKYRVNGPFVDVDEFYTAFNIKESSKMYRPDTARVRIW"
     gene            complement(3767..>4613)
                     /locus_tag="PK977_13785"
     CDS             complement(3767..>4613)
                     /locus_tag="PK977_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRF19239.1"
                     /translation="LESKKQFVEKFAFTDPERYWHYLHHLFPQNNFLVCALDIAAWDL
                     YGKLKGKQLYEMWGGDISKNPVCDYTIGIDTIDKMLAKMKEKPWPIYKIKVGTADDIA
                     IVRALRENTNAELRVDANAAWSLETALKLIPQLKELGVELVEQPLAKDDWEGMKVLYR
                     KSELPLYADEACVLEQDVEKCKDHFHGINIKLTKCSGITPARRMIQKARELDMKVMIG
                     CMNESTVGAAAIAHLLPFIDHVDMDGPLLLEEDLATGITYDFGKIGYSGMPGLGINYT
                     GIYQK"
     assembly_gap    4614..4623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4624..4801)
                     /locus_tag="PK977_13790"
     CDS             complement(<4624..4801)
                     /locus_tag="PK977_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRF19240.1"
                     /translation="MKVQYWPYSLKFRHPFTISKGTKTHQPTLIVELDHMGIKGYGEA
                     CAIAYYNIPVEKMIS"
     gene            complement(4817..>5799)
                     /locus_tag="PK977_13795"
     CDS             complement(4817..>5799)
                     /locus_tag="PK977_13795"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRF19241.1"
                     /translation="GIWFDYQRYCRPFITDEVLTEVFGDLAERVKGEYVVPNDFGGYD
                     LELKFETQKQVESHFAALEESPENIRIRDGLYDLISNVILFDDEEKPGEHFHFRISME
                     KTSSFRHLIPHIQEKFKELSINYFFRRQDDFWFKEAMHKLPALKEATNMLVCGEDLGM
                     VPHCVPDVMSQLGILSLEIQRMPKDPKKEFFHPNDAPYLSVITPSTHDMSTIRGWWEE
                     NRERTQQFYNHVMGQWGDAPYFCEAWINRAIVLQHLYSPAMWSIFQLQDILGMSETLR
                     RENPQEERINNPANPKNYWQYRMHIPLEELIKQKEFNDELKGYVENSGRG"
BASE COUNT         1705 a         1246 c         1110 g         1643 t
ORIGIN      
        1 acggatacaa tcaaacaagg tatttgcttc ttcaaaagtg cagtatttac cgacttaatc
       61 tcggaaaaaa ctgattccag gaaaccatcc ggcaactgtt ctgaagttga agcagattga
      121 taattatcat ctgtttcttc aatcattttt gcggttccgt caatacacat tttacctcca
      181 aatcccattt tactgcagct atgatccaaa acatccattg gaccggtaga gaaataaata
      241 tctgtagcag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natattttgc tagcgattca
      301 tagtcctgta tccgtacctt ttcgtcagcc agtaccaaaa ttttattaaa catcatctga
      361 cctgcacccc acatggcatt cattaccttt tgcccctgcc ccgcatattc tttattgatt
      421 tttgtaataa caaggttgtg aaaaacacct tctaccggca tgtccatatc cataatttcg
      481 ggcaccattg tcattttgat aggcgccagg aagatccgct cagtggcttt gcctaaccag
      541 gcatcctcct ggggaggcat tcctacaatg gtagcagggt agacaggact tttcttatgg
      601 gttatggcag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnatga tcgccaaaag gcccttccca gatcagttca tcagttggat caacataacc
      721 ttcaataata aaatctgcat ctgtaggaac ttcaatatca ggctggctta tacatttaac
      781 taattccact ttcttcttcc ttaaaaaacc agccagcata tattcatcca cattttccgg
      841 taatggcgct gtggcagaat aggcgtaaac cgggtctcct cctaatgcta cggctaccgg
      901 cattttctta ttcagctttt tgtactccga aaaatgtttc gcagatactt tgtgtttatg
      961 ccagtgcata cctgtaagcc ttgggccgaa cacctgcata cggtacatac ccacattacg
     1021 gctatttgta tttgggtctt tagtatgaat aactggcagt gttacaaaag gtcctccatc
     1081 cttaggccaa caggtaatta ccggaagttt tgtaatatcc gggtttccca ttatcacctc
     1141 ctggcattcg cctcgtccac tctttacttt aggcatccaa ctggcaaact gtcctaattt
     1201 tggcagcatt ttcaacttat ccagaatccc ttctttggga gcagccagca tcttgaacag
     1261 ctcctcaatt tcccttgcca catcatccaa atggtttaca ccaagcgcca tacacatccg
     1321 cttttcactt ccgtatgcat tcatcaacac aggaaaatca taccctgtat tttcaaacaa
     1381 caaagcctta ccgccgccac ccgatttgct gattctgtcg gtaacctctg caatttctaa
     1441 ttttggattt acataagtct gtatacggac caactctcct gctttttcga gggcttcaat
     1501 gaattgctgc tgattcttcc aggccatata ttgttttaaa agtgcgaagt tacaacacca
     1561 cccctgagta tttgttcaga aacaaactat tcttgctatc aaacaacccg tttatcaaat
     1621 ttgtagtgat agcctgtttg atttccttat tttagtcatt cataaacaat aaacaaatga
     1681 tgcgatcaaa gtattttctt ctggccctgg tatcaggcgc ccttcttttc gtttcttgta
     1741 aaacctcttc taaaaaagaa gacagtaagc cagatgtgct ggcggctaat cttgatacta
     1801 caataaaccc tgccgatgac ttttttatgt atgccaatgg agggtggata aaaaagaacc
     1861 ctataccagg cgatcaatcg tcctggggga ttggcaacct ggtaatagaa gaaaacctga
     1921 aacgactcag ggaaatttct gaaaaagctg aaaaagcaaa agcagtaaaa ggaacacctg
     1981 aacaaaaaat tggagatttc tggactactg ctatggacag cgcaaaaatt gaagcggatg
     2041 gattaaaacc acttaagcct tttctggata aaataaacgc catcactgat ataaaatcac
     2101 ttgtcaacac tgtagcagat ttaaagaaag caggatcatc taccttgttt agtgattttg
     2161 taacgcagga tgacaaaaac agtgagataa tgacttataa actctggcaa ggtggaattg
     2221 ggttgccgga aagggaatat tatttcaaaa cagacagcac aacagtaaat attcgcaacg
     2281 catatgtaaa atatattact gatatactga cgatgtctgg tgaagatgct gtagcagcac
     2341 aaatcgcagc caagaatatt ttggcacttg aaaccaaact ggcacaagcc agccggaaat
     2401 tagaagatct gcgggatccc tataaaaact ataataaaat ggctgtaacg gacctgggta
     2461 aaatgaacgc atctgttgac tggtcttcat acctgtctgt taccgggata aaaaatcttg
     2521 attcagttat tgtgggccag ccagaatttt ttaaagccct tgacaatgcg ttaaaaacag
     2581 taccgctggc ggactggaag agttatgtaa aattcaacct gattactgac tttgcgggag
     2641 cattgcccga tcaatatggg attgctgctt tcaatttcag caaactgttt agcggtgcca
     2701 aagaaagaaa accaagatgg aagagagtca tttcaagcga agaaagtgta atgggtgaaa
     2761 tgctcgggca actatatgta aaagaatttt ttaatgaaaa agctaaaaaa aggtatgaag
     2821 acctggtaga agctatccgg gatgcgttaa aagaacgtat tggcaaactg acctggatga
     2881 gtgatagtac caagcaaaag gcgtatgtaa aacttgcggc tatcaagaaa aaagtaggtt
     2941 acccggacaa atggaaagat ttcagtagta tggaaattgg cagggaaagt tatgtgcaaa
     3001 acctggtgaa tgccaataac tggtggcata attatgagat aaacaaactg ggcaaaccag
     3061 ttgaccggga tgaatggggc atgtatccac aaacctataa tgcctattat aatccttcca
     3121 ataacgagat tgtattacca gccggaattt ttacagtgcc agggtacagg gatgaagaat
     3181 tagacgatgc cactgtgtat ggctatgcag gagcttccac tatcggtcat gagataacac
     3241 atggttttga tgatgaaggc cgccagtttg atgcaaaggg caaccttgtc agttggtgga
     3301 ctaccaaaga tgaagaagag ttcaaaaaaa gagccgaggt aatgatccaa cagtttaatg
     3361 aatatgaacc ggtaaaagac tatcatatca atggtaaagc aacccttggc gaaaacattg
     3421 ccgacctggg tggtatttta ttaggcattg aggcttttaa aaaaaccaag caataccagg
     3481 aaaacaaaac actgggtggc ctcactccta tgcaacgata tttttgcggc tatgctttgg
     3541 gctggctggg ccatacccgt gaagaaaact tgaaaaatca attactgaca gatgtacatt
     3601 ccccggcaaa ataccgggtc aacggtccct ttgtggatgt agatgaattt tacactgctt
     3661 ttaatataaa agaaagttca aaaatgtaca ggccggacac cgccagggtg agaatctggt
     3721 aaataacagc cttttaatag aaaactccgg tgcaaaccgg agttttctat ttctggtata
     3781 ttcctgtata atttattccc aatccgggca ttcctgaata cccgatcttg ccaaaatcat
     3841 aggtaatacc ggttgcaagg tcttcttcca acaaaagcgg accgtccata tcaacatgat
     3901 caataaaagg aagcaggtgg gcaatggctg cagcacctac agtggattcg ttcatacaac
     3961 caatcatcac cttcatatcc aattcccttg ccttttgaat cattcggcgg gcaggtgtta
     4021 ttccgctgca tttcgtaagc ttaatattaa tgccgtgaaa atggtcttta catttttcca
     4081 catcctgctc caatacgcag gcttcatcag catataatgg cagttcagat tttctataca
     4141 gaactttcat tccctcccaa tcgtcctttg ccaatggctg ctccaccaac tctactccca
     4201 gttctttcaa ctgcgggata agctttaatg cggtttccag gctccaggct gcgtttgcat
     4261 ccactctcaa ttcagcattg gtgttctccc tgagagctct tacaatagca atatcgtctg
     4321 cggtacccac ttttatttta tagattggcc atggtttttc tttcattttg gccaacatct
     4381 tatcaatagt atcaatgcca atagtgtaat cgcaaacagg gttcttcgaa atatctccac
     4441 cccacatttc atacaattgc tttcctttta attttccgta caaatcccat gcggcaatgt
     4501 ccaaagcgca gacaagaaag ttattttgag gaaacaggtg gtgcaggtaa tgccaatacc
     4561 gctcaggatc agtgaaagca aacttctcta caaactgctt ttttgactcg aggnnnnnnn
     4621 nnncggagat cattttttct accggaatat tgtaataggc aatggcacag gcttcgccat
     4681 atccttttat acccatatga tccagttcga caataagagt tggctgatgt gtttttgttc
     4741 cttttgaaat tgtaaaagga tgacggaatt tgaggctgta gggccagtat tgcactttca
     4801 tacagacaaa gataacttat cctcttccgc tgttttcaac atatcctttc aattcatcat
     4861 tgaattcctt ttgcttaata agttcctcta aaggtatatg catccgatat tgccagtagt
     4921 ttttagggtt agccgggtta tttatgcgtt cctcttgagg gttctctctc ctcagggttt
     4981 cactcatacc cagaatatcc tgtaactgaa agatactcca catagcaggc gaataaaggt
     5041 gctgcaatac aatagccctg ttgatccatg cttcacagaa atacggagca tcgccccact
     5101 ggcccatcac atgattatag aactgttgtg tacgttccct gttttcctcc caccatcccc
     5161 gaatggtact catatcatgt gttgatggag ttataacaga taggtaagga gcatcattgg
     5221 ggtgaaaaaa ttctttcttt ggatctttcg gcatccgctg tatttccaaa cttaatatac
     5281 ctagctggct cattacatca ggcacacaat gaggaaccat accaagatcc tcaccacata
     5341 ccagcatatt cgttgcttct tttaaagcag gtagcttatg catggcttct ttgaaccaaa
     5401 agtcatcctg tctgcggaaa aaatagttta tagaaagttc tttgaatttt tcctgtatat
     5461 gcgggataag gtgtctgaat gaagaggttt tctccatgga aatacggaaa tgaaaatgct
     5521 caccgggttt ttcctcatca tcaaaaagta taacgttaga gataagatca taaagaccat
     5581 cacgaatcct gatattctcc ggtgattctt caagtgctgc aaaatgcgac tcaacctgtt
     5641 tttgagtttc aaactttagt tcgaggtcat accctccaaa atcatttgga acgacatatt
     5701 cccctttcac tctttcagcg aggtcaccaa acacctctgt aagcacttca tcggtgataa
     5761 agggacggca ataccgctga taatcgaacc atattcctt
//
LOCUS       DAORLS010000200         5779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7577_length_5779_cov_1.845038, whole genome shotgun sequence.
ACCESSION   DAORLS010000200 DAORLS010000000
VERSION     DAORLS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7577_length_5779_cov_1.845038"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..601
                     /locus_tag="PK977_13800"
     CDS             161..601
                     /locus_tag="PK977_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19242.1"
                     /translation="MRKIILVFSIAVISSCGSGLADKKVPELANEMCDCFTAFQKRFD
                     SESASLLKEVSTSSDPKTLFQEGVRKMTPEKADFFTRQIQSLGDKSSSVYACLSDFDK
                     KHGKETTKSKSELKKKLLVELQKRGSCFTAAAMVNLDIHRNSDQ"
     gene            complement(610..1356)
                     /locus_tag="PK977_13805"
     CDS             complement(610..1356)
                     /locus_tag="PK977_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19243.1"
                     /translation="MNTAIDHRNIVNKPRKVLRNSIILALLMIAAAVISGYSLDGMRG
                     GYALIVIFGFIALTFIIVAFIYYPRAREFDRLVNDMNPLAHWTFSEEEWKLFTEEDLK
                     ESVDANKASLKMLIIISAIICGILALVYKDSFFIYILAGIIVFYGVIAMTVPHIKKKS
                     LLKGNRDVIIGKDSIIVGSSFQTWKFLGAKLRGAVVSNESNIPLLLIRVDFPTRHGYQ
                     ESVIRFPIPFGKLKDAEQIAQLLEKKTVTD"
     gene            complement(1367..>4829)
                     /locus_tag="PK977_13810"
     CDS             complement(1367..>4829)
                     /locus_tag="PK977_13810"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRF19244.1"
                     /translation="EQALQLNPGDNTAKTQLEQTKKWQQEWLAIEGKASALDAGINNK
                     NIPDAEKALNEIKKLQLNMPGKADNKWTKDKTDRFNAFTRSYDSAYANLKSKYTKSTN
                     DKDFHGALNLMEEFNKEWKGTPARLKDAQADIQFCKTQIAEQNRIYDNFLFTKEKFEK
                     GVPIDPKQTPAFIESNAQTKFGTQDPRQKEMIDFARAMDKKQKEYIINKEKAAQLKNE
                     GQQAETAGNKDEALKKYKESIALVPDSELDNKIKKLETEIAAINAKRNKADALWNEGE
                     QLAKKKKTKPEGLNKMKESLEWWNSPERIEKARELEKDVNGSYSKTDISGTWKHGSSE
                     TFTFTLTGDNLYNAVEKGFGNANGTVTMIGQLGIINYTTKDGTNGQYILKVTADGSSG
                     TGKWTDIRNNTGERNFTRVSRPDVKEPEVTKPEEKKKKGIGGFLDKVNKGLEKIDSVI
                     TGKKPDDKGIEPADASEVIIFKNGNIGGVSSGPTKPTTFTLSKATFITRIENYHYFNG
                     GRKPGTIAVRNSSGKTYGPWQAYGLIGQGGVQNAYWDVKPNMELPAGTYTIIDSDPST
                     WSYNSESKGCGFTTVWTNVAVKEPDKNDDTGINPADASKEVEIFNNGNIGGVSSGPTK
                     PTTFTLSKTTHITRIENYHYFNGGKKPGTIAVKNSSGKIYGPWQAYGLVGQGGVQNAY
                     WNVTPNINLPAGTYTVIDSDPATWSYNSESKGCGFTRIWTSTAVTQPEKPDETGVNPA
                     NTNEEEKIFDNGNKAGVYNGPTSPTVFTLSKTTYITRIENYHYFNRGKKPGTIGLKSS
                     TGKVYGPWQAYGIIGQGGVQNALWVVQPRIQLPAGTYTVIDSDPSTWSQNSGSKGCGF
                     TAVWAPKGNKTVPKTNDPGYTIEKVETGGGVTAEKIFITGDIDNLGFGFPKNFDVFSG
                     NSTPSHGYPWKINPADPPGTDRIMVGTGARGNADGYASNTTRPDNQPQPITLLCPLNG
                     ITVNSAVLQLFVDDFQSPYFGSKFTATINNKKADFLSSVLNGLSQTGPIGKLISLKVP
                     NEFISEIRTGKLVIYIDDATTGVGDGYAIDFVRLIINPTSFTYTGTITGIVYRPDGQP
                     AAGASINAGGIISTTANNKGEFTLNNVPAGMITINATYNNHHKKTINADLAAGKTIKL
                     TINLPAD"
     assembly_gap    4830..4853
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4854..5712)
                     /locus_tag="PK977_13815"
     CDS             complement(<4854..5712)
                     /locus_tag="PK977_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19245.1"
                     /translation="MGESEQLELVAEKPGFSITFNPAKAVVGQQVTAKIVSTPEKLEE
                     VNYRWMPLPSNAQLIRESQDGSEIIFYAKDALQITVESNAIVKGSGEELGKAKAYFNA
                     ERYNVKAEGPKVQGPIPMIWKEGVGLVEVDKEIAVHQIVEFTAVVSPAPSSSLTYDWK
                     VTQGNASISNPSSKDARVTALETGTIKITVTVKDNNGVVLGNASGAFNATISNEMISN
                     SAKMKKDFDDKMTKARQYLREGNIDEAVKLGLELKSMNAKEAANLINELVQACRKSAT
                     DAIAERDFKL"
BASE COUNT         1403 a         1218 c         1084 g         2050 t
ORIGIN      
        1 acagttgcgg cagtttgaac cataactgaa atagttattt agcagcaatg taaacgacag
       61 ttatatggtt gggcgctgca tttgcttata aaagcaatac atttattaaa gctggtgtgt
      121 tgcggacaat tgcatctgta ttttgtacaa aaatttattt atgcgaaaaa ttattcttgt
      181 ttttagtatt gcagttatta gttcctgcgg aagcggctta gccgataaaa aagtacccga
      241 actggccaat gaaatgtgtg attgtttcac cgctttccag aagcgtttcg acagtgaatc
      301 tgcttcattg ttaaaagaag ttagtacttc atctgatcct aaaacccttt tccaggaggg
      361 agtcaggaaa atgacgccgg aaaaagcaga ttttttcacc cgccagatac aatcgctggg
      421 tgacaaaagt tcatccgttt atgcatgtct ttcagatttt gacaagaagc atggaaaaga
      481 aactacgaaa agtaaaagtg aattaaagaa aaagttactg gtagaattac aaaagagagg
      541 aagttgcttt actgcagctg ctatggttaa ccttgatatt catcgtaatt cagatcaata
      601 gtaatgttat taatcagtca cagttttttt ctccagtaat tgggctattt gttcagcatc
      661 ctttagtttg ccaaaaggaa taggaaatct gatgacggac tcctggtaac catgcctggt
      721 tggaaaatca accctgatca acaggagggg gatattactc tcatttgaga ctacagcacc
      781 cctgagcttt gccccaagaa atttccaggt ttggaatgaa ctgccgacaa taatagaatc
      841 ttttccaatt atcacatccc tgtttccttt taacaggctt tttttcttaa tatgcggcac
      901 tgtcatcgca attaccccgt aaaaaactat aataccagcc aaaatgtaaa taaagaagga
      961 gtccttataa accaacgcta atattccaca aataatggca gatattatta tcagcatttt
     1021 cagcgatgct ttgttggcat ctactgattc tttaaggtct tcttcggtaa aaagtttcca
     1081 ctcttcttcg ctgaatgtcc aatgggcaag ggggttcata tcatttacaa gcctgtcaaa
     1141 ttccctggcc cttgggtaat atataaatgc aacaataata aaggtgagtg ctataaaccc
     1201 aaaaatgaca atcagggcat atcctcccct cattccatca aggctgtatc ctgaaataac
     1261 agctgctgct atcatcagca aggcaaggat gattgagtta cgaagtactt tcctgggttt
     1321 gttgactatg ttcctatggt caatagcggt attcatagat cttattttaa tctgcaggca
     1381 ggttaatggt tagtttaatg gttttgccag cagcaaggtc agcgttaatc gtttttttgt
     1441 gatgattgtt gtacgttgcg ttgatggtaa tcatcccggc aggaacatta ttcagggtaa
     1501 actcaccctt attgtttgcc gttgtactga taattcctcc tgcattgatg ctggcgccag
     1561 cagctggttg tccgtccggt cgatagacta taccagtaat ggtaccagtg taagtaaaag
     1621 atgtcgggtt aatgataagc cgtacaaaat ctattgcata gccatcgcct actccggtag
     1681 ttgcatcgtc aatgtaaatc accaatttcc cggttcttat ttcactgata aactcattcg
     1741 gtactttcag gcttatcagt ttgccgatcg gtccggtctg gctcagcccg ttaagaacag
     1801 atgacagaaa atctgctttc ttgttattaa tagtcgcagt gaattttgat ccaaaatatg
     1861 gcgactgaaa atcgtcaaca aataactgga gcactgccga gttgactgta attccattta
     1921 gggggcaaag aagtgttata ggttgcggct ggttatctgg cctggtggta ttagatgcat
     1981 aaccatcagc atttcctctg gcaccagtac ctaccataat tctgtccgtt ccgggagggt
     2041 cagcaggatt tattttccag ggatatccat ggctgggagt agaattgccg ctgaacacat
     2101 cgaaattttt aggaaaacca aatcccaaat tgtctatgtc tccggttata aaaatttttt
     2161 ctgccgtaac gcctcctccc gtttcaacct tttcaatggt ataaccggga tcgtttgttt
     2221 tgggaacagt cttattccct tttggcgccc atacagcggt aaaaccacat ccttttgatc
     2281 cgctgttttg ggaccaggta gaagggtcac tgtctattac tgtatatgta cctgcaggca
     2341 gttgaattct gggctgtaca acccataatg cattctgtac accgccctgg ccaattatac
     2401 cataagcctg ccaagggcca tatacttttc cagtactgct tttcaaacca attgtacccg
     2461 gtttcttgcc acgattgaag taatgatagt tttcaatacg ggtgatataa gtagtttttg
     2521 ataaagtaaa taccgttgga ctcgttggac cgttatagac accagcttta ttgccattgt
     2581 cgaatatttt ttcttcttca tttgtatttg caggattaac acctgtttca tcaggttttt
     2641 caggttgtgt tactgctgtg cttgtccata ttcttgtgaa tccacatcct tttgattcgc
     2701 tattatatga ccaggtagcg ggatcactgt ctatcacggt ataagtacct gcaggtaaat
     2761 taatatttgg tgttacgttc cagtatgcat tttgtacacc accttgtcca acaaggccat
     2821 aggcctgcca tggaccgtaa attttaccac tgctgttttt tactgcaata gttcctggct
     2881 ttttaccacc attgaaatag tgatagtttt caattcgggt gatatgtgtt gttttgctaa
     2941 gtgtaaaagt ggtaggtttt gtcggcccgc tggatacgcc acctatgttt ccattattga
     3001 atatttccac ttctttagag gcatccgcag gattaatgcc tgtgtcatca tttttatccg
     3061 gctctttcac agctacgttt gtccataccg ttgtgaatcc gcatccttta gattcactgt
     3121 tatatgacca tgttgaagga tcgctgtcaa taattgtata ggtgcctgca ggtaactcca
     3181 tattcggttt aacatcccag tatgcatttt gaacaccacc ctggcctata agaccataag
     3241 cctgccatgg accataggtt ttgccactac tatttctaac agcaattgtt cctggttttc
     3301 tgccgccatt gaaataatgg tagttttcaa tccgggtaat aaatgttgcc ttgcttaaag
     3361 taaatgtggt gggtttagtt ggtccactgg atacaccacc aatattacca ttcttgaaaa
     3421 ttattacttc agatgcatca gccggttcta ttcctttgtc atctggtttt ttacctgtta
     3481 ttacgctgtc aattttctct aatcctttat tgactttgtc caaaaatcca ccgatacctt
     3541 tctttttctt ttcttccggt tttgttactt ccggttcttt tacatcaggc ctgctgactc
     3601 tggtgaaatt tctttcccct gtattatttc tgatgtctgt ccacttaccg gttccactgc
     3661 tgccatcagc tgttactttc agtatatatt gcccgtttgt tccgtctttt gtcgtgtaat
     3721 tgatgattcc caattggccg atcatggtaa cggtgccgtt tgcattgcca aaaccctttt
     3781 ctacagcatt atacaggttg tcacctgtta aagtgaaagt aaatgtttcg ctgctgccat
     3841 gtttccaggt accgcttata tcagttttgc tgtagcttcc atttacatct ttttccaatt
     3901 cccttgcctt ttcgattctc tcagggctat tccaccattc caggctttct ttcattttat
     3961 ttagcccttc tggtttagtt ttctttttct tagccagttg ttctccctca ttccaaagtg
     4021 catcagcttt atttcttttc gcattgattg cagcaatttc agtttcaagt tttttgattt
     4081 tgttgtctaa ctcgctatcc ggtactaatg ctatactttc tttatatttt tttagggcct
     4141 cgtctttgtt tccggctgtt tcggcctgtt gcccttcatt ctttaactgg gctgcttttt
     4201 ctttatttat tatatattct ttttgttttt tgtccattgc tctggcaaag tcaatcattt
     4261 ccttttgtct tggatcctgt gtgccaaact tggtttgtgc attactttca ataaaagcag
     4321 gagtttgttt agggtctatt ggcacccctt tttcaaattt ttcttttgta aaaagaaaat
     4381 tgtcgtaaat cctgttttgt tcagctatct gggttttaca aaattgtata tcggcctggg
     4441 catctttcag tcttgcaggt gtgcctttcc attctttatt aaattcttcc attaagttca
     4501 atgcaccatg aaaatcttta tcatttgtgc ttttagtata tttcgattta aggttggcat
     4561 aagctgaatc ataactcctg gtgaaagcat taaacctgtc agttttatct tttgtccatt
     4621 tattatctgc tttgcctggc atgtttaact gaagtttttt gatctcattc aaagctttct
     4681 cagcatccgg tatatttttg ttattaatac cggcgtcaag agcagaagct ttgccttcaa
     4741 tagccagcca ttcctgctgc cattttttag tttgttccag ttgtgttttc gctgtattgt
     4801 cgccggggtt tagctgcaac gcctgctcgn nnnnnnnnnn nnnnnnnnnn nnnccagttt
     4861 aaaatctctt tctgcaattg catcagtagc tgattttcgg caagcctgaa ccagctcatt
     4921 aattaaattt gctgcttctt ttgcattcat gctttttaac tctaaaccaa gttttaccgc
     4981 ttcatcaata tttccttccc gcaggtattg tcttgctttc gtcatcttgt catcaaaatc
     5041 ctttttcatc ttagcactgt tgctgatcat ttcgttgctt atagttgcat tgaatgcacc
     5101 ggaagcgttg cctagaacaa ctccgttatt gtctttaacg gttacagtta ttttgatcgt
     5161 tcctgtttcc agtgcagtaa cccttgcatc ttttgatgac ggattgctga tggaagcatt
     5221 cccctgtgtt actttccaat cataagtaag agatgaggaa ggtgcagggc ttacaactgc
     5281 agtaaattct actatttggt gtacagcaat ctctttatca acttctacca atccaactcc
     5341 ttctttccag atcattggta tggggccctg cactttcgga ccttctgctt ttacattgta
     5401 tcgttcggca ttgaaatagg cttttgcttt acccaattct tctccgcttc cttttactat
     5461 tgcatttgat tcaacagtaa tctgcagggc atcttttgca tagaatatta tttcacttcc
     5521 gtcctgggat tctcttatta attgtgcatt ggatggcaaa ggcatccacc ggtaattgac
     5581 ttcttccagt ttttcgggag tacttacaat ttttgcagtt acttgttgtc ctactactgc
     5641 ttttgccgga ttaaaagtga tagaaaatcc gggtttctct gctactaatt ccaactgttc
     5701 agattcaccc atagtaattg tttctgcagt tgatttgtcc aacacctgca cccatacttt
     5761 tttattgccc ggtttagtg
//
LOCUS       DAORLS010000201         5767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7597_length_5767_cov_1.840861, whole genome shotgun sequence.
ACCESSION   DAORLS010000201 DAORLS010000000
VERSION     DAORLS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7597_length_5767_cov_1.840861"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>703)
                     /locus_tag="PK977_13820"
     CDS             complement(<1..>703)
                     /locus_tag="PK977_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF19246.1"
                     /translation="PFLLYGNSDNVKLGQWVLAIGYPLTLETTVTAGIVSAKGRNIGI
                     NQRQSKTPIEAFIQTDAAVNQGNSGGALITTDGQLVGINSAIYAPTGTYAGYSFAIPV
                     NLVKKIVNDLIKYGDVKRPYLGVWVAEDKVDAGDGAHIGNVAKDGAAAAIGLKPGDIV
                     TKINDATITSWTELQATVSSYNAGDKINITYKRNGKEYTATATLTAKSGTFDEIATNT
                     VGEKLGADLVTIDKK"
     assembly_gap    704..896
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<897..1253)
                     /locus_tag="PK977_13825"
     CDS             complement(<897..1253)
                     /locus_tag="PK977_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRF19247.1"
                     /translation="MKLKQILLIVAISAVSAVSSVWIYGKITRKQPNFVQSENGNIPA
                     NYAGFFDNAAGAGEPIDFTKAANAAVPAVVHIKTKIPAKRISNQLPRSRGNSMEDWFN
                     QFFDFGPQIQPEQRASG"
     gene            complement(1461..2459)
                     /locus_tag="PK977_13830"
     CDS             complement(1461..2459)
                     /locus_tag="PK977_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRF19248.1"
                     /translation="MNLEYRIDLMNKLGEYMQSGNRDWVAALEKAGRENGWFIPEFIE
                     TSVKNIAASFLKKKNLENLVQAYQLSAINQSPKTVGIVMAGNIPLVGFHDFLCVFIAG
                     HKAIIKPSSKDQVLIKQLVGKLIEWEPALASQIDFSEMLKGCDAYIATGSNNTSRYFE
                     YYFGKYPHIIRRNRTSVAILTGQETESELGKLADDVYLYFGLGCRNVTKIYVPQDYDF
                     VPLLEVFKKYNYLADHHKYKNNYDYNLALHILNKKFYMTNGSVLLIEEPALFSPISQL
                     NYEFYTDKNLLAEQLLQNPDMQCLVSKDHVPFGGAQSPSLTDYADGIDTMAFLRDL"
     gene            2614..2964
                     /locus_tag="PK977_13835"
     CDS             2614..2964
                     /locus_tag="PK977_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF19249.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAIADGTSVKGSFT
                     LLDGTVVDAEQKNAPVSVDTYYITPNKCTECQGFHEEPQCAAVCPVDCCVPDEMYQET
                     VDQLIAKKDKLHIA"
     gene            3289..>4177
                     /locus_tag="PK977_13840"
     CDS             3289..>4177
                     /locus_tag="PK977_13840"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRF19250.1"
                     /translation="MKKNIALVTGGFSGEAVISYRSANTIDNNLDRNLFNVYKIDINP
                     EGWHYIQQDGTRIAIDKNDFSILLPDGKVTFDAVYIGMHGTPGEDGKLQGYFDTLKIP
                     YTGCDAASSAITFNKGYAVAVAAMAGIAVSRSIVLLKNNSYKAEEIKDRLKFPVFVKP
                     NNGGSSIGMSKVDKPTDDLAGAIEKAFKEDDQVLVEEMINGREFTVGVFKSKGNIIVL
                     PLTEVMIHESKSFFDFEAKYEGKSTETTPAEVDEAAADKIRDAAKKIYAVFNCKGVIR
                     IDFIYNEAEGKPYMLEINTI"
     assembly_gap    4178..4189
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4190..4290
                     /locus_tag="PK977_13845"
     CDS             <4190..4290
                     /locus_tag="PK977_13845"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19251.1"
                     /translation="QSEASVVPQQVRAMGWSLKEFYTKIVMECFPY"
     gene            4325..>4567
                     /locus_tag="PK977_13850"
     CDS             4325..>4567
                     /locus_tag="PK977_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRF19252.1"
                     /translation="MLKFITQRPLWVNILVGIVLALGLFFLFLLSLNWLTGHGKAATV
                     PSVTGKSYEEARKILKKAGFDVDIQDSIYVDTARAMS"
     assembly_gap    4568..4580
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4581..5141
                     /locus_tag="PK977_13855"
     CDS             <4581..5141
                     /locus_tag="PK977_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRF19253.1"
                     /translation="QFPDADEVVKSNRTVYLVISRAIPPEIEMPNLVGYSFRNAEMVL
                     KNMDLKIGDTTFKPDFAKNAVLEQWYNGRIITPGTKIRKGSVISLVLGDGVGEREFTV
                     PVITGMLFGEAKEILEANGIVIGAIVTDANVTDTLGAWIYKQNPERFDDEKRIQKIRS
                     GQTIDVWLQVDKPVKDTTNPEIPSPL"
     gene            5155..5469
                     /locus_tag="PK977_13860"
     CDS             5155..5469
                     /locus_tag="PK977_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF19254.1"
                     /translation="MQTITVEELKARMDAGEKINLIDCREPSEYAEFNIGGKLIPLGK
                     IQTMQTDELDDLRDEEIIIHCRSGQRSMMACLFLDTLGFKNTKNLIGGMLAWQEKFGS
                     TI"
     gene            complement(5459..>5767)
                     /locus_tag="PK977_13865"
     CDS             complement(5459..>5767)
                     /locus_tag="PK977_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF19255.1"
                     /translation="PFSNFEIALISKYVSKQYLDNTQNENRVLNAFYVQDIRAMYTVK
                     PKWLKEISIIAQVNNVFNRKYEPNGYTFSYIYGGITTTKNYYFPMAGTNFLIALNIKL
                     "
BASE COUNT         1583 a         1097 c         1222 g         1647 t
ORIGIN      
        1 tgttttttat caatagtcac caggtctgcc cccaactttt cgccaacggt attagtggct
       61 atttcatcaa aggttcctga cttggcagtt aaggttgccg tagcggtata ttcttttccg
      121 ttgcgtttat aagtaatatt gatcttatca ccggcattat aggatgaaac ggtagcttgc
      181 aattcagtcc atgatgtaat cgttgcatca ttgatcttgg taacaatgtc acctggtttt
      241 aacccgattg cggcagctgc accatccttc gccacattac cgatatgggc gccgtcacct
      301 gcatctactt tatcttctgc tacccacaca cccaggtaag gccttttcac atcaccgtat
      361 ttgatcaggt catttacaat tttctttacc aggttaacgg gtatagcaaa cgaataaccc
      421 gcataggtgc cggtaggggc ataaatagca gagttgatac ctaccaactg gccgtcagta
      481 gtaatcagtg caccgccgct gttaccctgg tttactgcgg cgtcagtctg tataaaggct
      541 tcaattggtg tcttactctg acgctggtta ataccgatat ttcttccttt ggcgctaacg
      601 atacctgctg ttacggttgt ttcgagagta agcggataac caatggctaa tacccactgt
      661 ccgagtttta cattatcaga attaccatat aagaggaagg ggannnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntccc
      901 gaagcccttt gttccggctg aatctgcgga ccgaaatcaa agaactggtt gaaccagtct
      961 tccatactat tgccccggct gcggggtaac tggttactga ttcttttggc cggtatcttg
     1021 gtcttaatgt gcacaacagc cggtacggct gcgttggcag ccttggtaaa atcaattggc
     1081 tcaccggcgc cggctgcgtt gtcaaaaaag ccagcgtaat tggcaggaat attcccgttt
     1141 tcagactgaa caaaattggg ctgtttgcgg gtgatttttc cataaatcca cacactgctt
     1201 acagccgata cggcgctaat ggcaacgatc agtaagattt gtttcagttt catagtgtta
     1261 tatctttttt tcgtttaaat gcttttggat aatgcaggta atcaaaacct atgcctttgc
     1321 tttacaaaat aacaaactgt cataccaagt ttgttgaccg tcaatttagt ttaacaaatc
     1381 tttactaaga ctttcgtact tcaaatatcc cataaaaata ctattcaatc attgtcgctg
     1441 tataccgtta tgtacggatt ttacaggtcc ctgagaaaag ccatcgtatc tataccgtct
     1501 gcataatctg tcagtgaggg tgactgagcc ccgccaaagg gaacatgatc tttactgaca
     1561 aggcattgca tatcggggtt ttgtaaaagt tgttctgcca gtaaattttt atcggtataa
     1621 aattcataat tgagctggct gatgggcgaa aaaagtgccg gttcttctat gagcagaaca
     1681 gaaccgttgg tcatgtaaaa tttcttattc aatatgtgga gtgccaggtt atagtcatag
     1741 ttgtttttat acttatgatg atcggcgagg tagttatact ttttgaaaac ctctagcaga
     1801 ggtacaaaat cataatcctg gggcacatat atctttgtca cattgcggca gcccaatcca
     1861 aagtacaggt acacatcgtc tgccagtttt cccagttctg attcggtttc ttgcccggtt
     1921 agtatggcca ctgaggtgcg gttccggcgg ataatatggg gatatttacc aaaatagtat
     1981 tcaaaatacc tggaagtgtt attgctgccg gtagcaatat aggcatcgca gcctttcagc
     2041 atttcgctaa agtcaatttg actggccagt gccggttccc attctattag ttttccgaca
     2101 agctgtttga tcaatacctg gtcctttgag gaaggcttta ttattgcctt gtgcccggct
     2161 ataaagacgc ataaaaaatc atgaaagcct accaggggta tattgccggc cattacgata
     2221 ccgacagttt tcggtgattg gttaatggct gaaagttggt atgcttgtac caaattttca
     2281 aggtttttct ttttcaaaaa ggatgcagca atatttttta ccgatgtttc tataaactcc
     2341 ggtatgaacc acccgttctc ccgtccggct ttttccagtg cagcaaccca gtctctgtta
     2401 ccggactgca tatattcgcc cagtttattc atcagatcta ttcggtattc taagttcatt
     2461 tccctgtaat aaaattgaga gtaagttaat tttaaaccgg atgcaaatta gtcgggctgg
     2521 ctgtaaataa aaattgccaa accaattcat ccggattgga aaacagttaa ttttgtcatg
     2581 caaaagtaat tctcaacccg ttaatcaatt tttatggcta tcaagatcac tgaagaatgc
     2641 atcaactgcg gtgcctgcga acccgaatgt ccgaataatg ctatttacga aggtggcgta
     2701 gaatgggcta ttgccgacgg tacatctgtc aaaggttctt ttaccctgct ggatggaaca
     2761 gtggtagatg ctgagcaaaa aaatgcccct gtttctgttg atacgtatta catcacccct
     2821 aataaatgta cagagtgcca gggctttcat gaagagcctc agtgtgcagc tgtgtgcccg
     2881 gttgactgtt gtgttcctga tgaaatgtac caggaaaccg ttgatcagct gattgccaag
     2941 aaagacaagt tacatattgc ctaaggccgg gccaatgtgc ataaattctg cattgatttt
     3001 ttgcattcgc ctcgtttttg atataacttt atttcatacc taatatggtg cagcagcgag
     3061 agctgccgaa tgagtgacgg gtgatatttc ccgtcgtaaa aaggtgaagg ggaaacaacc
     3121 ttcacctttc ttatttgctg ctgaatttac tctgaaatat tccactttat catatttttt
     3181 gttaatgcta actgattaac aacctaatga ttgggcatct taaataattc tgcaatattt
     3241 gcatagctgt ttccgttttg cggaagccca aatccataca caaccacaat gaagaaaaat
     3301 atagcccttg ttacgggcgg cttttccgga gaagccgtaa tctcttaccg aagcgccaat
     3361 accatcgaca ataacctgga caggaacctg tttaatgttt ataagattga tattaatcct
     3421 gaaggatggc attatataca gcaggacgga accaggattg ccattgataa aaatgacttt
     3481 tctatccttt tacccgatgg taaagtaacc tttgatgcag tgtacattgg catgcatggc
     3541 acacccgggg aggatggcaa attgcaggga tattttgata cactaaaaat cccgtacact
     3601 ggttgtgatg cagccagttc tgctatcact tttaataaag gctatgctgt ggcggtggct
     3661 gccatggcag gtatagcggt ttcccggtca atagtacttt taaagaataa ttcttacaaa
     3721 gcagaagaga taaaagacag gctgaagttt cctgtatttg taaaacccaa taatggcggt
     3781 tccagtatcg gtatgtctaa agtggacaaa ccaacggatg atttagctgg tgctattgaa
     3841 aaggcattta aggaagatga ccaggtactg gtagaagaaa tgatcaatgg cagggaattt
     3901 acagtaggtg ttttcaagtc aaaaggaaat attattgtgc tgccattaac agaagtgatg
     3961 atacatgaaa gtaaaagctt ctttgatttt gaagcaaagt atgaggggaa atcaacagaa
     4021 actacgccgg cagaagtgga tgaagctgct gctgataaaa tcagggatgc agcaaaaaaa
     4081 atatatgctg tgtttaattg caaaggggta atcaggattg attttattta taatgaagca
     4141 gaaggtaagc cttatatgct ggagataaat accatacnnn nnnnnnnnng gcagagtgaa
     4201 gccagtgtgg tgccgcagca ggtaagggcg atgggctgga gtcttaaaga attttatact
     4261 aaaattgtaa tggagtgttt tccgtattaa tcgctccgct tataaatact aatctgacct
     4321 tttcatgctg aaattcatca cacaacgtcc tttatgggtt aatatcctgg tgggtattgt
     4381 actggctttg ggattgtttt tcttattcct gctttcgctc aactggttaa ccggtcatgg
     4441 caaggctgct accgttcctt ccgttaccgg caagtcgtac gaggaagcaa ggaagatctt
     4501 aaaaaaagca ggctttgatg tagatataca ggattctatc tatgtggata cggcgagggc
     4561 catgtctnnn nnnnnnnnnn caatttccgg atgccgatga agtggtaaaa tcgaacagaa
     4621 cggtttacct cgtcatcagc agggctatac caccggagat agaaatgccc aatttggtag
     4681 gatatagttt ccgcaatgca gagatggtac tgaagaatat ggacctaaag attggtgata
     4741 caacttttaa gcctgacttt gccaaaaatg cagttttgga acaatggtat aatggcagaa
     4801 taataacacc aggtacgaaa atcaggaaag gaagtgtgat atcacttgta ttgggagatg
     4861 gagtaggcga aagagagttc actgtaccgg taataacagg tatgttattc ggagaagcaa
     4921 aggagatact cgaagccaat ggcattgtga tcggggctat tgttactgat gctaatgtta
     4981 cagataccct cggcgcctgg atatacaaac aaaaccctga gcgatttgat gatgaaaaaa
     5041 gaattcaaaa gatacggtcc ggccaaacga ttgatgtctg gctgcaggta gataaacccg
     5101 tcaaagacac aacgaatcct gaaatacctt cacctttata atgtttgact aattatgcag
     5161 accattactg ttgaggaatt aaaggcccgc atggatgccg gcgaaaaaat caacctgata
     5221 gattgccgtg aaccgtcaga atacgctgaa tttaatatcg gcggcaaact cattccattg
     5281 ggaaagatac agacgatgca aaccgatgaa ctggatgacc tgagagacga agagatcatc
     5341 attcactgcc gcagcggaca gcgcagtatg atggcctgcc tgtttttgga tacattagga
     5401 ttcaaaaaca ccaaaaatct aattggtggt atgttggcct ggcaggaaaa gtttggttct
     5461 acaatttaat atttaacgct attaagaaat tggttcccgc catgggaaaa taatagttct
     5521 tcgttgttgt tatccctcca tagatataac tgaaggtata accatttggt tcgtatttcc
     5581 tgttgaatac attgtttacc tgtgcaatga tacttatctc tttcagccat ttgggtttca
     5641 ctgtatacat cgccctgata tcctgtacat agaatgcatt caataccctg ttttcattct
     5701 gtgtattatc gagatattgt ttgcttacat atttactgat gagtgcaatt tcaaaattgc
     5761 tgaaggg
//
LOCUS       DAORLS010000202         5702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7695_length_5702_cov_1.835842, whole genome shotgun sequence.
ACCESSION   DAORLS010000202 DAORLS010000000
VERSION     DAORLS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7695_length_5702_cov_1.835842"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>378)
                     /locus_tag="PK977_13870"
     CDS             complement(<1..>378)
                     /locus_tag="PK977_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF19256.1"
                     /translation="ATSADKKPNDDFVKQLTLNAEAAINMLDSIGVGDKNRMAVGGHS
                     YGAFMTANLLAHTKLFKAGIARSGAYNRTLTPFGFQNEDRSYWQAPELYNGMSPFSYA
                     DKIKTPLLLVHGDMDNNTGTFPI"
     assembly_gap    379..472
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<473..2261)
                     /locus_tag="PK977_13875"
     CDS             complement(<473..2261)
                     /locus_tag="PK977_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19257.1"
                     /translation="MNRRLCKLIIAFLVIATSASAQDDVSYKLPPKDIADMLMAKPTP
                     NISMDDKGQWMLLMESNSYPSVEELAKPELRIAGLRINPANFAPSRQNFINNLTLRSV
                     NFREDFKISGLPSPLFAGNVSWSPDDKKIAFTHTTKDRVDLYVIDVATKKATKINKAA
                     LNMIGGGYQWYDNNTLLYRTTLKPATAAPPKPVMPKGPTVQENYGQASPRPTFQDLLK
                     SPYDEQLYEFYATAQLVKNTNGVETKIGQPAIYGSITVSPDKKYLLQRIIKKPFSYTV
                     PANGFPANVVITDMTGKLVKQVADLPSSETAPGGYDNVQLVPRAFDWRDDEPATITWC
                     MPLDSGLLKVKAEYRDAVYALQAPFTGEATMLFKTKMRYSGTVWGDANLALVTEGLRS
                     KQLVQIDRFNSVTGDLEKLITRNTTNAYANPGNPVTEPNQYGKNVLRTIDKGNKILFN
                     NTTGSSPKGDLPFLMSYDVHTKKADTLWRCEEGFFESVVRVMNADKLELLTRRESESI
                     MPNYWIKNLKAKMADRQITFFTNPYPQLEGVTKEKIKYKRADGVDLTGDLYLPKGYNK
                     ERDGKLPVFIWAYPAEYNSAADAAQIRGSE"
     gene            complement(2339..3601)
                     /locus_tag="PK977_13880"
     CDS             complement(2339..3601)
                     /locus_tag="PK977_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19258.1"
                     /translation="MRKLLIALFCFFLQSSLFAQQGDKYILLKPDKVFDGEQLHSGWV
                     VLVKNNYIEAAGAMNFKFPAGTQIIELKGTTLLPGLIEGHSHLFLHPYNETSWDDQVL
                     KESRAERTARAVNHAKATLLAGFTTVRDLGTEGAMYDDAGLKKAIEKGVIPGPRMIVA
                     TRAIVARGAYGPKSESPDVDLPQGAEEVAGLEELSKAIRSQIGHGADVIKVYADYRYG
                     PDGETQPTFSDIELGWLVGNTISSGRKAVAHAASPEGMKRAINAGVATIEHGDNGTPE
                     VFKLMKEKNVALCPTLAAGDAIEQYLGWKKGTDPEPERITNKKKTFQQALDAGVTICM
                     GGDVGVYTHGDNAREMELMVEYGMKPLEVLKSSTSVNADVFGYAGKIGRIKKGLFADI
                     IAVEGDPSADIKNIRKLKLVMKNGVIYK"
     gene            complement(3610..3897)
                     /locus_tag="PK977_13885"
     CDS             complement(3610..3897)
                     /locus_tag="PK977_13885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF19259.1"
                     /translation="MIIHHKHVGSKGLFYVGEDGAFLAEMVYTMPAHDKMIIEHTEVS
                     EELKGQNVGYLLVNTAVEYARTHNLKIIPLCAFANAVFKKKPEYADVLYTS"
     gene            4095..5525
                     /locus_tag="PK977_13890"
     CDS             4095..5525
                     /locus_tag="PK977_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRF19260.1"
                     /translation="MKKIKIETVCKKMLADVYTPVGIYLRLRDKFRDTILLESTDHHS
                     AENSWSFICINAIGGIEVRSSETVEFKLPGRNPEKIALDKNSNVPKLMWEYMQRFDAV
                     TPAIKEGKFAQGLFGYTAYDAVQFFESVRLSRKPSLSVTEEAAIPPLRYRLFQYVIAV
                     NHFKDELFICENKISDVESDMNMVESLIRSKDVPVYPFHADGEESINITDEEYREMVK
                     KGIASCHRGDVFQIVLSRRFQQKFTGDEFNVYRALRSVNPSPYLFFFDYGDYKLMGSS
                     PEAQLVISNGKAIVHPIAGTFKRTGDDEADKLATERLLKDAKENAEHVMLVDLARNDL
                     SRLCDEVEVAHYRQVQYFSHVIHLISEVTGKLREGSNPFELLAKTFPAGTLSGAPKFK
                     AMELIDAYEPTARGYYGGAIGFIGFDGSCNHAIMIRTFLSRHNTLTYQAGAGVVAASN
                     PESELQEVNNKLGALKKAIEIAEKLF"
BASE COUNT         1407 a         1182 c         1388 g         1631 t
ORIGIN      
        1 tgtataggga atgtgccggt gttgttgtcc atatcgccat gcaccaacaa caaaggtgtt
       61 tttattttat ctgcatagct gaaggggctc atgccattgt acagttccgg cgcctgccag
      121 taagagcggt cttcattctg aaaaccaaaa ggagtgaggg tacggttgta agcaccgctg
      181 cgtgcaatac ctgctttgaa gagtttggta tgtgccagca ggttggcagt cataaaggcg
      241 ccataactgt ggccgccaac agccatcctg ttcttatcac ccacaccgat ggagtccagc
      301 atattgatag cggcttcggc attcagggtg agttgtttta caaaatcatc attgggtttt
      361 ttatcggcgc tggtagccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctcactg
      481 ccgcggatct gtgctgcatc agctgcagag ttgtattcgg cagggtaggc ccagatgaat
      541 acgggcagtt taccatctct ttctttgttg tatcctttgg gcaaatacag gtcgccggta
      601 agatccaccc catcggctct tttgtatttg atcttttctt tggttactcc ttccagctgc
      661 gggtaaggat tggtgaaaaa ggtgatctgc ctgtcggcca ttttagcttt caggtttttg
      721 atccagtaat tgggcatgat gctttcgctt tctcttcttg tgagcaattc cagcttgtcg
      781 gcattcatta cccgtacgac agattcaaag aaaccttctt cgcaacgcca gagggtgtct
      841 gcttttttag tgtgtacatc atagctcatc aggaagggca ggtcgccttt gggtgaactg
      901 ccggtggtat tgttgaataa tattttgttg cctttgtcaa tggtacggag cacatttttt
      961 ccgtactggt tgggttctgt tacggggttg ccgggattgg cataggcatt tgtggtgttt
     1021 cttgtgatga gtttttccag gtcgccggta acggaattaa acctgtcgat ctgcaccagt
     1081 tgtttgctgc gcaatccttc agtgacgagg gcaagattag catcacccca tacggtaccg
     1141 ctgtaacgca tttttgtttt gaagagcata gtggcttcac cggtgaaggg agcctgcagt
     1201 gcatacaccg catcccggta ctccgctttt actttcagca aaccactgtc gagcggcatg
     1261 caccaagtaa tggtggccgg ttcatcatcc cgccagtcga atgcacgggg aaccaactgc
     1321 acattatcat aaccaccggg agcagtttca gaagaaggaa ggtcggcaac ttgttttacc
     1381 agtttgcctg tcatgtcagt gatcactaca ttggccggaa agccattggc cggaacggtg
     1441 taagaaaaag gttttttaat gatgcgctgc agtaaatatt ttttatcggg cgacactgtg
     1501 atgcttccat agatagcagg ctgaccgatt tttgtttcca cgccattggt attctttacc
     1561 agttgtgcag tagcataaaa ctcatacaac tgttcatcat aagggctttt gagcaggtcc
     1621 tggaatgtag ggcggggaga ggcctggcca taattctcct gtacggtggg gcctttgggc
     1681 attaccggtt tgggaggagc agccgttgcg ggtttcaatg tagtgcggta gagtaaggta
     1741 ttgttgtcgt accactggta gccgccgcca atcatgttaa gcgccgcttt atttattttg
     1801 gttgcttttt ttgtggccac atcaattaca tacaaatcaa ccctgtcttt agtggtatgg
     1861 gtgaaagcaa tttttttatc atcggggctc cagctgacat tgccggcgaa taaaggagaa
     1921 ggcagtccgc tgatcttgaa atcctccctg aagttcacac tgcgcagggt aaggttattg
     1981 ataaagttct gccggctggg tgcaaagttg gccgggttga tacgcaggcc ggcgatcctc
     2041 agttcgggct tggccagttc ttctacggaa ggatagctgt tgctttccat aagcagcatc
     2101 cactggcctt tatcatccat actgatattg ggagtgggtt tggccatcag catgtcggca
     2161 atatcctttg gcggcagttt atagcttaca tcatcctgtg cagatgcgct ggtggcaata
     2221 actaaaaatg caataatcag tttacagagc cttctgttca tggtgtggaa attttagttc
     2281 ggaaagttaa gtaaggaaag gatttaaaat ataccggcag tggaaaaaac gcagattatt
     2341 acttatagat gaccccgttt ttcattacca gtttcagctt gcgaatattt ttaatatctg
     2401 cggagggatc gccttccacg gcaatgatat cggcgaacaa tccttttttg atccttccga
     2461 ttttaccggc atagccaaag acatcagcgt taacggaagt ggatgatttc aatacctcca
     2521 gcggtttcat accgtattcc accatcagtt ccatttctct tgcgttgtcg ccatgcgtat
     2581 acacacccac atcaccaccc atgcaaatgg taactcctgc atccagtgcc tgctgaaagg
     2641 tttttttctt attggtgatg cgttccggtt cggggtcagt gccttttttc cagccaaggt
     2701 attgttcaat agcatcaccg gcagcaaggg tggggcagag tgctacattt ttttctttca
     2761 tcagtttaaa aacttcgggt gtgccgttat caccatgttc gatggtagca acaccggcat
     2821 tgatagctct tttcattcct tcgggagaag cagcatgggc tacagctttt cgtccgctgc
     2881 tgatagtatt gcccaccagc cagcccagtt caatatcgct gaaagtgggt tgcgtttcac
     2941 catccgggcc atagcggtag tcggcatata cttttattac atctgcaccg tgaccaatct
     3001 gtgaacggat ggctttgctc agttcttcca gtcctgctac ttcttcggcg ccctgcggca
     3061 ggtctacatc ggggctttcg cttttagggc cataagcgcc acgggcaacg attgcccttg
     3121 tggctacaat cattctcggg ccgggtatta ctcctttctc aattgctttt ttcaaaccgg
     3181 catcatcata catggcgcct tcggtgccaa ggtctcttac ggtggtgaat ccggccaaca
     3241 gtgtggcttt agcatgatta accgccctgg ccgttctttc tgctcttgat tccttcaata
     3301 cctggtcatc ccaggaagtt tcgttgtaag gatgcagaaa aagatgggag tgaccttcga
     3361 tgagtcccgg caataaggtt gttcctttca gttcaataat ttgtgtaccg gcgggaaatt
     3421 tgaaattcat ggcgccggct gcttcgatgt aattattctt tacaagtact acccagccgc
     3481 tgtggagttg ttcgccgtca aatactttat cgggtttcag tagaatgtat ttatctccct
     3541 gctgtgcaaa aagggaagac tgtaaaaaga aacagaaaag tgcaatcaat aattttcgca
     3601 taaaaagaat taagaagtat aaagaacatc agcgtattcc ggttttttct taaacacggc
     3661 attggcgaag gcgcaaagcg gtatgatctt gaggttatgg gtgcgggcat actccacggc
     3721 ggtattcacc agcaggtagc ccacattctg ccctttcagt tcttcactta cttctgtatg
     3781 ctcgataatc attttatcat gagcaggcat agtgtaaacc atttcggcca ggaatgcacc
     3841 gtcttcgccg acataaaaca aacctttgga gccgacatgc ttgtggtgga tgatcatgtg
     3901 attgtaagtt ttattaaaac tacccaaaat tttttgctta tttgggtttg cctccacatc
     3961 ttgcgatgta atgaaaatga ttcaaacaaa taattggtgg tggcatacaa ctcttaaggg
     4021 gctgtaatgt cattactgta tttgtacaat aatattcagg ccccggttca tccggggttt
     4081 tcttttagca ggctatgaag aaaataaaaa ttgaaacggt ttgtaaaaaa atgctggccg
     4141 atgtgtacac accggtgggt atttatttac ggcttcggga caaattcagg gataccattt
     4201 tgctggagag tacagaccat cattcagcgg agaacagctg gtcgtttatc tgtatcaatg
     4261 ccattggcgg tattgaagta cgttcatctg aaacggttga attcaaattg cccggaagaa
     4321 atcctgaaaa gatcgcattg gataaaaaca gcaatgtgcc gaaactgatg tgggagtaca
     4381 tgcagcggtt tgatgcagtg accccggcaa taaaagaggg aaagtttgca cagggattgt
     4441 ttgggtatac ggcttatgat gcagtgcagt tttttgaatc agttcgcctg agccgcaaac
     4501 cttcgctgtc agttacggag gaggccgcaa ttcctcctct acggtatagg ttgtttcagt
     4561 atgtgattgc ggtcaatcat tttaaagatg aattattcat ttgtgagaat aaaatcagtg
     4621 atgttgagag tgatatgaat atggtggagt cgctgatcag gagtaaagat gtgcctgtat
     4681 atccttttca tgctgatggg gaagaaagta tcaatattac ggatgaggaa tacagggaaa
     4741 tggtaaagaa gggaatagcc agttgtcacc gcggtgatgt ttttcaaatt gtactgagcc
     4801 ggagatttca gcaaaaattt acaggagatg aattcaatgt gtacagggca ttacgaagtg
     4861 tgaatccttc tccttattta tttttctttg attatggtga ttataaactg atgggttctt
     4921 cgccagaggc gcaactggtt atttcgaacg gcaaagcgat tgtgcatcct atcgccggta
     4981 cattcaaaag aaccggcgat gatgaagcag ataaactggc aacggaacga ttattaaaag
     5041 atgccaagga gaatgcagaa catgtgatgc tggtagacct ggcaagaaat gacctgagca
     5101 ggctgtgtga tgaagtggaa gtagcgcatt accggcaggt gcaatatttt tcacatgtca
     5161 tacaccttat aagtgaagtg acaggaaagc taagagaagg cagtaatcct tttgaactgc
     5221 tggcaaaaac atttcctgca ggcacattga gcggcgcacc taagttcaag gcgatggaac
     5281 tgatcgatgc atacgaacca acagcccggg gttattatgg cggtgcgatc ggttttatcg
     5341 gtttcgacgg aagttgcaac catgctatta tgatccggac ttttttgagc aggcataata
     5401 cactcactta ccaggccggt gccggggtag tggcagccag taaccccgag agtgagttgc
     5461 aggaagtgaa taataagtta ggagcgttga agaaggcgat tgagatagca gagaaactat
     5521 tttgaatgtt gaattataaa ttttaattgt catgtttccc ttagccattc ctcattcagc
     5581 attcaaaatt taaaatagtc caggtaatga aaatacttgt tttcgataac tacgactcct
     5641 tcacctacaa cctggtccac ctggtagaga agataacgca taccaaagtg gatgtgtacc
     5701 gc
//
LOCUS       DAORLS010000203         5610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7881_length_5610_cov_1.756076, whole genome shotgun sequence.
ACCESSION   DAORLS010000203 DAORLS010000000
VERSION     DAORLS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7881_length_5610_cov_1.756076"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK977_13895"
     CDS             <1..561
                     /locus_tag="PK977_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRF19261.1"
                     /translation="IPYTIGNYLADEIAVKPIATDHPAFVETEKAVADRINTLMNIKG
                     SQTVESFHKRLGKIMWEKCGMARNEKGLKEAIAEIQQLKKEFWSDVRIPGAINEMNPE
                     LDKAGRVADFIELGELMCIDALDRNESCGGHFREESQTEEGEAKRDDANYSYVAAWEY
                     KGESQWQLHKEQLTFEVAKPTQRSYK"
     gene            563..>828
                     /locus_tag="PK977_13900"
     CDS             563..>828
                     /locus_tag="PK977_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19262.1"
                     /translation="MKVSGFTIIKNALKYDYPVVESIQSILPLVDEMIVCAGDSEDDT
                     NKLIESIGSEKIRIVHSVWQKYTEGGAALAVETNKAMDAVAADA"
     assembly_gap    829..914
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <915..1451
                     /locus_tag="PK977_13905"
     CDS             <915..1451
                     /locus_tag="PK977_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF19263.1"
                     /translation="DDKKVDGLLFHYHHFYGSYRYIGDSRKWYSKEIRVIRNDKTIRS
                     YRDAQGFRKINDEKLRVKLIEAWIHHYGWVKNPVFMQRKDRDFGQFWNDEKSQAAWAD
                     YIDRKGQEYDYSVIDSLEMFNGTHPAIMKKRVESESWHFRFDIKKKNFKNFKHRLLYY
                     FEKRTGIRPFEYSNYKRI"
     gene            1472..2233
                     /locus_tag="PK977_13910"
     CDS             1472..2233
                     /locus_tag="PK977_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRF19264.1"
                     /translation="MEHTSMNLTLKVWRQRNSQDTGSFETYEVKEVSEEMSFLEMFDV
                     LNERLIREGKDPIAFDHDCREGICGMCSMVIDGKPHGPWKANTVCQLHMRAFKDGDTI
                     VVEPWRANAFPVIKDLMVDRSSFDRIIQAGGYISVNTGNAVDANAIPIEKDKADAAFA
                     AAACIGCGACVAACKNSSAMLFLSAKVSHLALLPQGSPERKSRALNMVAQMDKEGFGS
                     CTNTGACEAVCPKEISIENIARLNREYMMAGAASE"
     gene            complement(2284..2838)
                     /locus_tag="PK977_13915"
     CDS             complement(2284..2838)
                     /locus_tag="PK977_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19265.1"
                     /translation="MKILSLLFLLLMQLKIKGQEASDKKANPGKDNLVSVGAAIPLGN
                     FSSTHLPGIGIEYAKSKNRFGILSIADKRKIAFVYGGGAAIYIGKKETVSLYPYKYPV
                     YIFIKTTAGVFYKPGSKSGFTFAAGPAIGIYNGNTQFNLTTKLDGSYYVNEKLAITPG
                     IFLMKEMTVADPLIAFSLKASLAL"
     gene            complement(2861..>4833)
                     /locus_tag="PK977_13920"
     CDS             complement(2861..>4833)
                     /locus_tag="PK977_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF19266.1"
                     /translation="SSTAISDSTGKLLFYTNGVTVYNRLHQVMLNGDNLKGNESACQG
                     ALITPMPGSDSLYYIFTTDAIENSFQKGYNYSVVDMSRDNGNGEVIEKNTPLWASCTE
                     RLAAARHANGVDVWIITNDNDSDIFRAWLLTCNGLQPTPVVSLAGVVLNQHIVTNTGY
                     MKVSPDGSQLCQTHLPLYDPDEINPNFIQLFDFNNNTGQITNARSIAFGNAMYVSCEY
                     SPDSRMLYLTRTYERKLDQLEAKLVNPVAIGNSTITFSTPDPVFSLQLGPDEKIYAFK
                     PDDVLGTINYPNQKGIACGYVHEQTSIAPRFSRYASPSCMNDVAYDPNNGFSFTIIDS
                     CTGTVQFNGLTGLTGTLVWEWDFGDGNTSTLQNPMHTFNPPNQAYEVKLKISSLSHCG
                     TIRSKRIIKPGGSIINADFDFVIRCDSGYIRFINKSVFAPNEATCLWDFGDGNTSVSI
                     NPVHTYAREGMYNVKLKLITGTNCLDDSMELQVDVKSFTVNVTPAIQTIVIGQPVFLS
                     TEEPANTYQWSPGKWLSDSATRSPVATPLEDMVYRLRASNNEGCTGEDSVIIKVIQYN
                     DIYVPTAFTPDNNGRNDILRPHFGIKYELKEFNIYNRWGVLVFTTQQLGIGWDGSYKG
                     NRQDAGVYIWLLKATDADGKIIEKKGNFLLIR"
     assembly_gap    4834..4843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5185..>5610
                     /locus_tag="PK977_13925"
     CDS             5185..>5610
                     /locus_tag="PK977_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19267.1"
                     /translation="MSCITSRAQVPATNPATSNLRIKSFKVTADSLVIDSISIIPSSF
                     KVAGIAPVDYRLDFVKAILYWNKRPVQDSILISYRVFPFQLNSVTQRMKYDSVMNNFY
                     VKPYEFNNGLTNAQRGVFDFGTLKAEGSFGRQIGFGNSQD"
BASE COUNT         1664 a         1029 c         1257 g         1564 t
ORIGIN      
        1 attccctata ctattggtaa ctacctggca gatgaaattg cagtgaagcc gattgcaacg
       61 gatcatcctg cttttgtgga aaccgaaaaa gctgtagccg acaggatcaa tacactgatg
      121 aacatcaagg gatcacaaac agtggaaagc tttcataagc gtttaggcaa gattatgtgg
      181 gagaaatgtg gtatggcccg taatgaaaaa ggattaaaag aagccattgc agaaatacaa
      241 cagttgaaaa aagagttctg gagtgatgtt cgaattccgg gagcaatcaa tgaaatgaat
      301 cctgaactgg ataaggccgg ccgtgtggct gatttcattg agttgggtga actaatgtgt
      361 atcgatgcat tggacagaaa tgaaagttgc ggcggccatt tcagggaaga aagtcaaaca
      421 gaagaaggcg aggcaaaaag agatgatgcc aattattctt atgtggcggc ctgggaatac
      481 aaaggtgaaa gccagtggca attgcataaa gaacagttga cgtttgaagt tgccaaacct
      541 acacaacgga gttataagta agatgaaggt tagcgggttt acaatcataa aaaatgcgtt
      601 gaagtatgat tacccggtgg tagagtcgat acaatctatc cttccgctgg tagatgaaat
      661 gattgtttgt gcaggagatt cggaggatga taccaataag ctcattgaat caatcggcag
      721 tgaaaaaatc aggatagttc actctgtttg gcagaaatat acagagggtg gcgctgcatt
      781 agctgtagaa acaaataagg caatggatgc agtggcggct gatgcggann nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnngatgat aagaaggtag atggtttgtt atttcattat catcatttct
      961 atggaagcta ccgctatatc ggggatagcc ggaagtggta ctctaaagag atcagggtta
     1021 tccggaatga taaaaccatc cgctcatacc gggatgcaca gggattcaga aaaattaatg
     1081 atgaaaaact cagggtgaag ttgattgaag catggattca tcattatggt tgggtaaaaa
     1141 acccggtttt catgcagcgg aaggacaggg attttggcca gttctggaat gatgagaaaa
     1201 gccaggctgc ctgggccgat tatattgacc ggaaagggca ggagtacgat tattccgtca
     1261 ttgattcact ggaaatgttt aatggtactc acccggccat catgaaaaag agggtggaaa
     1321 gtgaaagctg gcattttcgc tttgatataa aaaagaaaaa tttcaaaaat ttcaaacacc
     1381 gcctgttgta ttattttgaa aagcggaccg gaattaggcc gtttgaatac agtaattata
     1441 aaagaattta aaccctgata aatagttgaa catggaacat acaagtatga acctgacact
     1501 gaaagtatgg aggcaaagaa atagccagga taccggaagt tttgagactt atgaagtgaa
     1561 agaagtatcg gaagaaatga gcttcctgga gatgtttgat gtgctgaacg aaaggttgat
     1621 ccgtgaaggg aaggacccga tagccttcga ccacgattgc cgtgaaggta tttgtggaat
     1681 gtgttcaatg gtgattgatg gtaagccaca tggtccctgg aaggccaata cagtatgtca
     1741 gttgcatatg agggctttta aagatggtga taccattgta gttgaaccct ggagagccaa
     1801 tgcattcccc gttatcaaag atttaatggt cgaccgaagt tcattcgacc ggataataca
     1861 ggccggaggt tatatttcag tcaacaccgg taatgccgta gatgccaatg ctataccgat
     1921 tgaaaaagat aaggcagatg cggcttttgc agcagccgcc tgcattggtt gtggagcctg
     1981 tgtagcagct tgcaaaaatt cttcagccat gctgttttta tcagctaagg tttctcacct
     2041 ggccttatta ccacaaggct cacctgaaag aaaatcaagg gccttgaata tggtagcgca
     2101 aatggataaa gaaggctttg gctcttgtac aaataccggt gcctgcgagg ctgtttgtcc
     2161 aaaggagatt tcaattgaga acatagcccg cctgaacagg gaatacatga tggcaggtgc
     2221 ggcatcagaa taatgtaaaa tctatatcgt ttttagaagc ccttttgtca aagggctttt
     2281 ttgttataat gccagggatg cttttagtga gaatgctatc agcggatcag caacagtcat
     2341 ttctttcatc aaaaaaattc cgggagtgat ggctagtttc tcattgacat aataactgcc
     2401 gtccagtttg gtggttagat taaattgagt atttccgttg taaatgccga tggccggtcc
     2461 tgcagcaaat gtaaaaccag attttgagcc tggtttgtag aaaacaccgg cagttgtttt
     2521 tataaaaata tacactgggt atttatatgg atagaggctg acagtctctt ttttgccaat
     2581 ataaatggca gcacctccgc cataaacaaa ggcaattttc cttttatcag caattgagag
     2641 gattccaaac ctgtttttac tttttgcata ttcaatacct atacccggta aatgagtgct
     2701 ggaaaaattg ccaagaggaa ttgctgcacc aaccgatacc aggttatctt tcccgggatt
     2761 tgcttttttg tcggatgcct cctgaccctt tattttcagt tgcatcagca ataaaaaaag
     2821 tagtgatagg attttcatcg ggataaacta aggatacaag ttaacggata agcagaaaat
     2881 ttcctttttt ctcaatgatt tttccatctg catcagttgc tttaagcagc cagatgtaaa
     2941 cacctgcatc ctgcctgttg cctttatagc ttccatccca gccaatgccc agctgctgtg
     3001 tagtgaatac taatactccc caacggttgt aaatgttgaa ttctttcaat tcatatttta
     3061 tgccgaaatg cggacgtaaa atatcatttc tgccattatt atcaggagta aaagctgtag
     3121 gaacatagat gtcgttgtac tggatcactt tgatgattac agagtcttca ccggtacatc
     3181 cttcattatt agatgccctg aggcggtaaa ccatatcctc taaaggagtg gctacaggac
     3241 ttctggtggc agaatcacta agccatttac cgggagacca ttgataggta tttgccggct
     3301 cttcagtaga tagaaaaacc ggctgaccta tgacgatggt ttgtatcgcc ggcgtaacat
     3361 tcacggtaaa actctttaca tctacttgta attccatgga gtcatcaaga caattggtgc
     3421 ctgtgatgag tttcaatttc acattgtaca tgccttccct ggcataagta tgtaccggat
     3481 taattgacac agatgtgttt ccgtcgccaa aatcccataa acaggtagct tcattcgggg
     3541 caaaaacact cttattgatg aagcggatat agccggaatc gcatcttata acaaaatcaa
     3601 aatcagcatt gattatagat ccgccgggct taatgattct cttgcttctg atagtgccgc
     3661 aatgtgataa agaagaaatt ttcagtttca cttcatatgc ctggttgggc ggattgaaag
     3721 tatgcattgg attttgaagt gtagaagtat tgccatcccc aaagtcccat tcccaaacta
     3781 aagtaccggt aagaccagtc aggccattaa actgtacagt accagtacaa ctgtcaatga
     3841 tagtaaagct gaatccgtta tttgggtcat atgcaacatc attcatacag gatggggaag
     3901 catatcttga aaaacgggga gcaatacttg tttgctcatg cacatatccg caggcaattc
     3961 ctttttgatt gggataattt attgtgccaa gcacatcatc tggtttaaag gcataaattt
     4021 tttcatccgg gcctaattgc agggaaaata ccgggtcagg agtagaaaaa gtaatggtcg
     4081 aattaccaat ggcaacaggg tttaccagtt ttgcctccaa ctgatccaat tttctttcat
     4141 aagtccgggt aaggtatagc atccgtgaat caggagagta ttcgcaagaa acatacattg
     4201 cattgccaaa tgctattgat ctcgcattgg taatttgtcc tgtattatta ttgaagtcga
     4261 ataactggat aaagtttgga tttatttcat caggatcata aagtggcagg tgtgtctggc
     4321 aaagttgact tccatccgga cttaccttca tatacccggt attggtaacg atatgctggt
     4381 ttaagactac gccagccagg gaaaccaccg gggtgggctg aaggccatta caggttagta
     4441 accatgccct gaaaatatca gaatcgttat cattagtaat tatccagacg tcaacaccat
     4501 tggcatgcct tgccgctgcc aatctttctg tgcaggatgc ccataatgga gtgttctttt
     4561 caattacttc gccattgcca ttgtcacggc tcatatccac cacagaataa ttataacctt
     4621 tctgaaaaga gttttcaatg gcatcagtgg tgaatatgta gtataaacta tcactgcccg
     4681 gcatgggtgt gattaaagct ccctggcagg ctgattcatt tcctttcagg ttatcaccat
     4741 tcagcataac ctggtgaagc cggttataaa cagtaactcc attagtatag aacagtaatt
     4801 taccggtaga atccgaaata gctgtactgc tttnnnnnnn nnnaaatcat cgctccgttt
     4861 gtaagtgcaa aaggaagggg ctgattggta acattattaa aacttaaacc agctttggct
     4921 ccaaagtacc agttattgtt ctgttgagcc aacatcaccg ggtgatgaca aagcagcaaa
     4981 aggattataa tagtaatctt tctcaagact gacccgggtt tatgtcattt ctatgatgaa
     5041 gtggtaatca aaattgctgc ttttgagcgg aattaacagc atgggataac tttacagcaa
     5101 taaaaggacg aattttacga ttttgagcta aacctgctgc tattgaagtg gttatgtgca
     5161 atatcctgcc ttattttact aaccttgtcc tgcattacaa gcagggcaca ggtacccgct
     5221 actaatcccg ccacttctaa tcttcgaata aagtctttta aggtaacagc agatagcctg
     5281 gttattgaca gcattagtat cattccttct tcatttaaag ttgcaggaat tgcccctgtt
     5341 gattaccggc tcgattttgt taaggcgatt ctttactgga ataaaaggcc tgtacaggac
     5401 agtattctta tttcatacag ggtttttcct tttcagttga attcagtgac acagcggatg
     5461 aagtatgaca gtgtgatgaa taatttctac gtaaaacctt acgaatttaa taatggattg
     5521 accaatgcac aacgtggtgt atttgatttt ggaacattga aagcagaggg aagttttggc
     5581 cggcaaatcg ggtttggcaa cagccaggat
//
LOCUS       DAORLS010000204         5589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7906_length_5589_cov_1.641850, whole genome shotgun sequence.
ACCESSION   DAORLS010000204 DAORLS010000000
VERSION     DAORLS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7906_length_5589_cov_1.641850"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK977_13930"
     CDS             <1..950
                     /locus_tag="PK977_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF19268.1"
                     /translation="LRLLIHAYKVAGINKTTEIGIINTEGAGYLGMGENAIIQPGYVA
                     DKPNPFYNTYYRTVAGAQTANNVYYRANSWGIDYYTYNADPRRSRFYEAGSNGYVGVA
                     YGLPPINANASSNLAAIGPGLYKTNTAGQLIMSSAESYFLQAEAAWRGWIPGGAPTVT
                     SLTNTGIQESFTYMGAGSAAGYISGNAGYSDVDVKAVSNTPANIPTLTPPVGGLFTII
                     SQKWFALNGLNTLEVWTDYRRLEYKEVVPSSSVTDRFVYGAGVGYDPGPPISVSPQNT
                     STKIPVRYLYPQSEYNYNAANVGAQGTIDRYTKIFWDIN"
     gene            975..1892
                     /locus_tag="PK977_13935"
     CDS             975..1892
                     /locus_tag="PK977_13935"
                     /inference="COORDINATES: protein motif:HMM:NF020111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HRF19269.1"
                     /translation="MKKILSTTTLIIALSVAFTSCLKDKGFENNEYGINDPDTQPPGV
                     GFPLAANKKNTVGLNVSGSSQVVNDILFVNLNAGITAPSDIQVTLALDSQIVKDYNTA
                     NGTAIKVMNYSLFTLQSTTVTIPAGGRNAQIAINVPSTLMLDPNASYGVGVKISAVSG
                     NYVIAGNLKNLMIEFTIKNKYDGRYNLRGYHNRPTLDLPYDENVNMITTGPNSVNMFW
                     PALGGLAHPLNGGTTYYGSFTANFIFDLNTNVMTGWDWTPYPTTLPTTVGPGSRYDPT
                     DKIIYFYGWYNNNPGARAFFDTLRYLGPR"
     assembly_gap    2022..2081
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2133..>2426
                     /locus_tag="PK977_13940"
     CDS             2133..>2426
                     /locus_tag="PK977_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19270.1"
                     /translation="MVSRAPDSRGWTDPALNHNRLLQQQAADGNYKLIGPYKVIGSQY
                     LFGEHHKADMFTPEAKAYNIFVSYNTYNQEVEFYSTSNPNTPLVREPGTVDSFT"
     assembly_gap    2427..2579
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2580..2798
                     /locus_tag="PK977_13945"
     CDS             <2580..2798
                     /locus_tag="PK977_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19271.1"
                     /translation="LAQSELRQFDLQVEYYYTARDNKGLKKLKANAPSVIKEFKDVKD
                     LSSVITADMFTANPEEALIKAFNSLNEQ"
     gene            2839..3543
                     /locus_tag="PK977_13950"
     CDS             2839..3543
                     /locus_tag="PK977_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19272.1"
                     /translation="MRNIISIILLFIGSAAVAQSGYMDNALNYNRLIRERTGDGVYKV
                     IGNFRVVGTSYLFGERNKGNLFSTEAKAYNIYLSYNTYNQEVEFYSSANPDKPLVKEP
                     GTVDSFVINDNIEVGIIKPIKFVYATHIGATDKFYYQEIVSGGRFSLYKRYKSELGYV
                     STNYVQSELRQFDMTYDYYYSDNTSKGIKKLKANPGFVTKEFKGIKDVSGIATSDAFS
                     MNPEETLIKVFSTLNN"
     gene            3564..>3789
                     /locus_tag="PK977_13955"
     CDS             3564..>3789
                     /locus_tag="PK977_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRF19273.1"
                     /translation="MNDQQPIPNQLNIEISEEIAEGAYANLAIITHSHAEFVIDFVNV
                     MPGTPKSKVKSRIILTPQHAKRLMKALTENI"
     assembly_gap    3790..3918
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3975..>4303)
                     /locus_tag="PK977_13960"
     CDS             complement(3975..>4303)
                     /locus_tag="PK977_13960"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF19274.1"
                     /translation="LTGKICFMGDYKDLHAFKKAYDLAMEIFVLSKEFPPEEKYSLTD
                     QIRRSSRSVCTNIAEAYRRRRYKDYFLSKLNDCESENAETQVWLDFAKDCGYISKKTY
                     LSISEK"
     assembly_gap    4304..4413
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4414..5046)
                     /gene="radC"
                     /locus_tag="PK977_13965"
     CDS             complement(<4414..5046)
                     /gene="radC"
                     /locus_tag="PK977_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRF19275.1"
                     /translation="MQEHKYSIKHWAKDDRPREKLLLNGAENLSNSELLAILIHNGSR
                     EKSAVDLAKEVLKLGKDNLSELGRLSIKDLMKIKGIGEAKAITIAAALELGRRRQSAN
                     PLQKTIISTSRDIADYLQARLKDYRHEVFAVLFLNRANKINHFEIISEGGITGTVADP
                     RIILRKALEADAVNIILCHNHPSGSLKPSRADEQLTSKIKEAARLLDIAVI"
     assembly_gap    5078..5087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5196..5402
                     /locus_tag="PK977_13970"
     CDS             5196..5402
                     /locus_tag="PK977_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19276.1"
                     /translation="MKTKTFSPVGSLHPVLFFAVVYVVAIVFSIFICSTLFYSCNASP
                     GKLGQQQEVPVEKPVNGPTEMASR"
BASE COUNT         1463 a         1072 c         1062 g         1530 t
ORIGIN      
        1 aactgcgtct gctgatacat gcgtataaag ttgctggtat caacaaaacc actgagattg
       61 gtattatcaa tacagaaggt gctggttacc tgggtatggg cgaaaatgcc ataatacagc
      121 ccggatatgt agccgacaag cccaatccgt tctataatac ctattacaga actgttgctg
      181 gtgcacaaac agctaataac gtttactaca gggcaaacag ctggggaatt gactattata
      241 catataatgc tgatccccgc aggtctcgct tctatgaagc aggatcaaat ggttatgtag
      301 gagtagctta tggattgcct cccattaatg ccaatgcatc aagcaacctg gcagctatcg
      361 ggcccggttt gtataagact aataccgctg gccagttgat catgtcttct gctgagagtt
      421 atttcctgca ggctgaagct gcctggagag gatggattcc cggcggagcc cctacagtta
      481 catcattaac taatactggt atccaggaga gctttactta tatgggtgcg ggcagtgctg
      541 caggttatat ctccggcaat gcaggctact ctgatgtgga tgtaaaagct gtatctaata
      601 cacctgctaa tatcccaact ttgactcctc cggttggtgg tcttttcaca ataatttctc
      661 aaaagtggtt tgccctgaac ggtttgaaca ctttggaagt atggacagac taccgcaggc
      721 tggaatacaa agaagttgtg ccatcatctt ctgtaactga caggtttgta tatggtgctg
      781 gtgttggtta tgaccccgga cctccgattt ctgtatcacc tcagaacact tctactaaga
      841 ttcctgttag gtatttatat cctcagtctg agtataacta caatgcagcg aatgttggtg
      901 cacagggtac aatcgacagg tatacaaaaa tattctggga catcaactaa tcatacactc
      961 ttaaatttat gaatatgaaa aaaatacttt caacgaccac cttaatcatt gccctttcgg
     1021 tcgcttttac cagctgtctg aaagacaaag gctttgagaa taatgagtat ggtatcaacg
     1081 accctgatac acagcccccg ggagtagggt ttcccctggc tgccaataaa aagaacactg
     1141 taggtctgaa tgtgtcaggt tctagccagg tagtgaatga tatcctgttt gtaaacctga
     1201 atgcaggtat tacagctcca tctgatatcc aggttacttt ggctttggat tcccagatcg
     1261 ttaaggatta caataccgct aatggcactg ccattaaggt aatgaactat tcattgttta
     1321 cgctgcagag cacaactgta actataccag ctggtggcag aaatgcgcag atagctatca
     1381 atgtaccaag cactttaatg ctggatccca atgcatcata cggagtaggt gttaagattt
     1441 cagccgtttc tggtaattat gtaattgcag gcaacctcaa gaacctgatg attgagttta
     1501 ctatcaagaa taagtatgat ggtcgttata acctgagagg ttaccataac aggccaactt
     1561 tggatctgcc gtatgatgag aatgtaaata tgattactac gggtccaaac agtgtgaata
     1621 tgttttggcc tgctttaggt ggtcttgcac atccgcttaa cggtggtacc acttactacg
     1681 gtagttttac agctaacttc atctttgatc ttaataccaa cgttatgaca ggttgggatt
     1741 ggacgcctta tcccactaca cttcctacta ctgtaggacc cggcagccgt tatgatccta
     1801 ctgataagat catttatttc tacgggtggt ataacaataa tcctggagca agagcattct
     1861 ttgataccct tcgctacctt ggaccaagat aatttttaga gtagtatgat tttgcaaagg
     1921 gctgcctaaa aggcagccct ttgtttttaa agtattggta aaaaaatcaa taagttctgc
     1981 aggccgctgc tgttttgtac atttgatgaa agtaaatagc annnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatgcacaa cagctacaac
     2101 atccagtcgc tttatcaagt aactcaaaca caatggtatc aagggcgccg gacagccgtg
     2161 gctggaccga ccctgctttg aaccacaaca ggttacttca gcaacaagct gcagatggca
     2221 attacaaact tattggtcca tataaggtta taggttcaca gtatcttttt ggagaacacc
     2281 acaaggcaga tatgtttacg ccggaagcta aagcatacaa tattttcgta agctacaaca
     2341 cctacaacca ggaggttgag ttttattcta cttccaaccc taacaccccg cttgtaaggg
     2401 agccgggcac ggtggactcc tttacgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2581 ttgctcagtc agagttacgc cagtttgacc tgcaggtgga atattattat accgccagag
     2641 acaataaggg tctgaaaaaa ttaaaagcta atgcaccatc ggtaattaaa gaatttaaag
     2701 atgtaaaaga tctttcttca gtaattactg ctgatatgtt taccgccaac ccggaggaag
     2761 cactcattaa agcctttaat tctttaaatg aacagtaaac tattttgcat ttaattcgtc
     2821 tttaatcaaa ttcttatcat gcgtaacatt atttctatta ttcttctttt tatcggcagt
     2881 gcagcagtgg cccaatctgg atatatggat aatgccctta attacaacag gcttatccgt
     2941 gaaagaaccg gtgacggggt gtataaagta attggcaatt tcagggtggt gggcacttct
     3001 tacctgtttg gagaaagaaa taagggtaat ttattcagca cggaggctaa agcctataat
     3061 atttatctca gttataatac atacaaccag gaagttgagt tttactcttc tgctaaccct
     3121 gataaaccct tagtgaaaga acccggaacg gttgattctt ttgtgatcaa tgataatatt
     3181 gaagtgggaa tcattaaacc cataaagttt gtttatgcca cgcatatagg agcaacggat
     3241 aaattttatt accaggagat tgtaagcggg ggcaggtttt ctttatataa aaggtataaa
     3301 tcggagctgg gctatgtgtc taccaactat gtgcagtcag aattaaggca gtttgatatg
     3361 acatacgatt attattattc tgacaacaca tcaaagggaa tcaaaaaact aaaagccaat
     3421 cccggctttg tgaccaaaga attcaaaggc attaaggatg tatcaggtat tgctacttca
     3481 gatgcttttt ctatgaaccc ggaagaaacg cttattaaag tatttagcac cctcaataat
     3541 taattcacct tacaaatacc gatatgaacg atcagcagcc gatcccaaac caactgaata
     3601 ttgaaatatc agaagaaatt gctgaaggtg cttacgccaa cctggccatt attacccatt
     3661 cacatgctga atttgtcatt gattttgtaa acgtaatgcc aggtacaccc aagagcaaag
     3721 taaaatcaag gatcatattg acaccacaac atgccaagcg gctaatgaag gcccttacag
     3781 aaaatatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnat gaacgaaatt tatccgggtt tttaatcata taccagatta
     3961 attttcctgc ttcatcattt ttcgctgata gataaatatg ttttttttga gatgtagccg
     4021 cagtcttttg caaaatcaag ccatacttgt gtttcggcat tttccgattc acagtcattg
     4081 agtttgctta gaaagtaatc tttatatcgt cttcttctat aagcttcagc aatattagta
     4141 caaacagatc ttgaagatct tctgatctga tcagttagcg agtatttttc ttccggagga
     4201 aattctttcg atagaacaaa aatctccatt gccaggtcat atgctttttt aaatgcatgc
     4261 agatctttat aatctcccat aaaacatatt ttgcctgtca gtgnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntattacg gcaatatcaa gcagtcttgc
     4441 tgcttccttt atcttagatg ttagctgttc atctgcccgg ctgggtttca ggctgcctga
     4501 gggatgatta tggcagagaa ttatgttgac ggcgtccgct tctaacgcct tccttaaaat
     4561 gatccggggg tcggctacag tgcctgttat gcccccttca ctgataatct caaaatggtt
     4621 gatcttattg gccctgttca ggaagagaac agcaaaaacc tcatgccgat aatcttttaa
     4681 ccgggcttgc aggtagtcag caatatccct gctggtgctg atgattgttt tttgcaatgg
     4741 attggctgac tgccttctcc ttcccaattc aagggcagcg gcaatggtga tggccttggc
     4801 ttctccgata cctttgatct tcatcaggtc ttttattgac agacggccca gttcactcag
     4861 gttgtctttg ccgagtttaa gtacttcttt ggccaggtca accgccgatt tttcccggct
     4921 gccattatgg atcaggatgg ccagcaactc agaattgctg agattttctg caccattcag
     4981 tagtaatttt tccctggggc ggtcgtcttt tgcccaatgc ttaattgagt acttgtgctc
     5041 ttgcattgct ccaaattagc taatattttt attccatnnn nnnnnnnaac aaggttagcc
     5101 aataatttta tccctgacaa tacccgaaag tggtatttgt accaggtcac caataaacgg
     5161 atccaccctt tgaaaaacac taaaatctgg tagatatgaa aaccaagaca tttagccctg
     5221 tgggttcact gcacccggtt cttttttttg cagtggtata tgtggtggcc attgttttct
     5281 ccattttcat ttgctccaca cttttttata gctgtaatgc aagcccggga aaactaggcc
     5341 agcagcagga ggttccggtt gaaaagcctg taaatggccc aacggagatg gcttcccgtt
     5401 aattggctgt ttagggtcaa tattttttgc ggcatactgt ggcgattggg tggagaataa
     5461 tatcttcgcc gcacattttc ctgtcatgca atatgccaaa attttctccg gaaccggctc
     5521 gcaaaagcta gccgaacaaa tctgtaaacg ttatggtaca aagctgggaa aggtcaacat
     5581 ccagcggtt
//
LOCUS       DAORLS010000205         5577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7924_length_5577_cov_2.006519, whole genome shotgun sequence.
ACCESSION   DAORLS010000205 DAORLS010000000
VERSION     DAORLS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7924_length_5577_cov_2.006519"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..677)
                     /locus_tag="PK977_13975"
     CDS             complement(135..677)
                     /locus_tag="PK977_13975"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF19277.1"
                     /translation="MKKLYVLLVAAGLFVAGSASAQTKIAYIRIDDIVGLMPELAPEK
                     VNMDTVGQQFIKDSIMPRIQYVQGEYQRKLGEYTDTTKPAGVREQILKDLQSYKDELD
                     GAESLIQQVQQYKQQELLRPFYAKAKKAIEQVAKEKGYTHVMNSDTFLVAPEADDISL
                     LVLTKLNIKLPNQPAKPGTK"
     gene            complement(759..1274)
                     /locus_tag="PK977_13980"
     CDS             complement(759..1274)
                     /locus_tag="PK977_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF19278.1"
                     /translation="MKKIFIAAVSFLLLAFTATAQKYAIIDTRYILDKMPDYKEAQKQ
                     LDEIAAGWQKDIDGKQADLDKMYKDFEAEQVMLTEELKKKREDQLFNKEKELRDLQRK
                     RFGFEGDLFKKRQELIKPIQDKVYNAVQKMAVARGYDFVLDKSEGITIIFADPKLDKS
                     EDILKDLGVRN"
     gene            complement(1338..>3339)
                     /locus_tag="PK977_13985"
     CDS             complement(1338..>3339)
                     /locus_tag="PK977_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRF19279.1"
                     /translation="ESPYGQKKKYSFSQYTKDWGFLSLTKTKRLLDPWFRFKLFSGAK
                     FDAKKYEEDKEKVLDYYNSLGYRDAQIVGDTQYTTPKGNLNIDLKVEEGRKYYFGNIA
                     WKGNSKFSDSLLNVILGINKGDVYNISILNKRLGKEASQDGGGDISGYYMDDGYLFFR
                     AEPVETAVYNDTIDYEIRITEGPQARIKNVTIAGNEKTKDYVIRRELRTIPGELFSRT
                     DLIRSQRELANLQFFNQETIDPGVVPNAEDGTVDINWKLEEKSSDQLELSAGWGGGIG
                     LTGTLGVTFNNFSIRNIWKKSSWDPLPTGDGQKLSLRVQSNGRAFRSYNFSFTEPWLG
                     GKKRNSLTIGVNSSKFSNAFDPFTGTIDRARSDTNYLKTTGLSLALGKQLKWPDDYFS
                     LVYTMSFTQYKLRNYPIFDQDFRNGTSNNFSMKIALQRSSVFDPIFPRSGSNFMASVQ
                     FTPPYSLFNKNISKTNDKYKNPEYHKWRFNAEWYVPIGKPMGADKNRQFVLKMAAKYG
                     FMGRYNKDLDYSPFERFQVGDAGLTNNFGLLGYDIIAHRGYPVYQNSDPTINPDQQSA
                     SQFFTIFNKYTLEMRFPLVTNPSSTIYALSFFEAANGWYNYKDYNPFRLRRSVGVGMR
                     FFLPMFGLLGFDYGVGLDRITPGQSGLKGASRFTFMLGFEPD"
     assembly_gap    3340..3396
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3397..4094)
                     /locus_tag="PK977_13990"
     CDS             complement(<3397..4094)
                     /locus_tag="PK977_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HRF19280.1"
                     /translation="MQRFLPRLCALLTAAVVSVFSVQAQEDTTKPTSLDPKLIEWEKA
                     QVPKEYTIADVKITGVKHLDTSIISSITNILPGDKFVHPGADIFAKAIANLWRQKFFS
                     YVQIYVTRVEGDKVWVEVNVQERPRLGNFKFVGISKTDAEELTTRASLAKQTIITENT
                     RRSITEIVTKYYTEKAYRNVKVTIDEFTDSSFVNSNQMVITVDKGKKVRINEIAFFGN
                     ESVDGLRLKKQMKGT"
     gene            complement(4269..5027)
                     /locus_tag="PK977_13995"
     CDS             complement(4269..5027)
                     /locus_tag="PK977_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRF19281.1"
                     /translation="MSALVEQIDKQRLPRHIAIIMDGNGRWAKEKGEDRLFGHFHGVE
                     SVRNIVEGCAELGVEYLTLYAFSTENWDRPEYEVVGLMELLVNTIRKEVESLNKNNIR
                     LHVIGDMSMLPDYAKKELDEALEITRKNTGLNLIMALSYSGRWELLNAVKNIAYEVKS
                     GRLNVEAINQDTLQRYLCTSGFPDPELMIRTSGEYRISNFLLYQLAYAELYFTNVRWP
                     DFRKDNLYEAIIDYQGRERRFGKTSEQVTVEDKA"
     gene            complement(5142..>5577)
                     /locus_tag="PK977_14000"
     CDS             complement(5142..>5577)
                     /locus_tag="PK977_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19282.1"
                     /translation="FITIKINFMRLTKTLLLLSVISLFSFTTISKESAGKPGDWFFLG
                     DKNVGFGVDHDVIHFGNWKDDVRQIKLKITDGPLKMYNMKIHFDNGSVQEVALRNRFA
                     QGSESRVIDMDGGLRHLTKIEFWYETRGFARGKSRVAIWGRN"
BASE COUNT         1496 a         1253 c         1029 g         1742 t
ORIGIN      
        1 cctccataac cggaatcaaa tacacctatt ggaccttgct gctgcataat gcaaaaatag
       61 tgatgtgctt tctggctata aacaataaat cccatctgtt aaccagatgg gatttaccac
      121 tagaaaaact caatttattt cgttccgggt tttgccggtt gattgggcag tttaatattc
      181 aacttggtaa gaacaagcag cgaaatatca tccgcttcgg gagccacaag gaatgtgtca
      241 gagttcatta catgcgtata gcctttttcc ttggccacct gctcaattgc ctttttagcc
      301 ttcgcataaa aaggtctcag taattcctgt tgtttgtatt gctgtacttg ctgaataagt
      361 gattcggcac catccagctc atccttatag ctctgtaagt cttttaagat ctgttctctt
      421 acccctgctg gtttggtagt atctgtatac tctcccagtt tacgctggta ttcaccctgt
      481 acatattgga tacggggcat aatagagtct ttaataaact gctgacccac ggtgtccata
      541 ttcacttttt caggagccag ttctggcatc aaccctacga tatcatcgat acggatataa
      601 gcgatcttgg tttgtgcaga tgcagaccct gccacaaata atcctgctgc tacaagcaat
      661 acatataact ttttcatttg tgaatttttc tttctgtaga ttgtattaaa aaataattgg
      721 ttgcgctgtt ttgatttatt taacacccct tcttttgatt aatttcttac tcccaggtct
      781 tttagaatat cctcactctt gtccagtttg gggtcggcaa agataatggt aattccctca
      841 cttttatcga gcacaaaatc gtagcctcgg gccacggcca tcttctgtac cgcattgtaa
      901 accttgtcct gtatgggttt tatcagttcc tgtctctttt tgaacagatc accttcaaaa
      961 ccaaagcgtt tgcgctgcag gtccctcaat tctttttctt tattgaataa ctggtcttcc
     1021 cttttctttt tcaactcttc tgtaagcatt acctgctcgg cttcaaaatc cttgtacatt
     1081 ttgtcaagat cggcctgctt tccatcaata tctttttgcc acccggcagc aatttcatcc
     1141 aattgctttt gcgcctcttt gtagtcaggc attttgtcaa gtatatatct tgtatcgata
     1201 atagcatatt tctgagccgt tgctgtgaaa gcaagcaaca ggaaagaaac agctgcaata
     1261 aatatttttt tcataacaag catatttaaa tgaacagacg ctgaaagtag aaaaaaagtt
     1321 tagttactta aatgatatta atctggttca aagccaagca tgaaggtaaa ccttgacgcc
     1381 cctttcaggc cggattgacc cggtgtgata cgatccagac ctacaccata gtcaaatcca
     1441 agcaatccaa acatcggcag gaagaaacgc atacccacac ccacactacg gcgcagacgg
     1501 aatggattgt aatctttata attataccag ccgtttgctg cttcaaagaa gcttaacgca
     1561 tagatagtac tgcttgggtt ggtaactaag gggaaacgca tctccaaagt atacttattg
     1621 aaaattgtaa agaactgaga tgcactttgc tgatctggat taatcgttgg atcagagttt
     1681 tgataaacag gataaccacg gtgtgcaatg atatcgtaac ccaataaacc aaagttgttt
     1741 gtcaaacccg catcacccac ctggaaccgt tcaaacggtg agtaatcgag atccttattg
     1801 taacgaccca tgaaaccata cttggctgcc attttcaata caaactgtcg gtttttatca
     1861 gcacccattg gtttaccaat cggtacatac cattcagcat tgaagcgcca tttatggtat
     1921 tccgggttct tgtacttatc gtttgtcttt gaaatattct tattaaacaa tgaataaggc
     1981 ggtgtaaact gtacgctggc catgaagttg gaaccgcttc tcgggaaaat aggatcgaat
     2041 acagaagaac gctgcaaagc gatcttcata ctgaagttat tggaagtacc gtttctaaag
     2101 tcctggtcaa aaatcgggta gttgcgcaat ttatattgcg taaagctcat agtatacacc
     2161 aggctgaaat aatcatccgg ccattttagt tgtttaccca gtgccaggga caatccagtt
     2221 gtcttcaggt agttggtatc ggaccttgca cggtctattg ttcctgtaaa aggatcaaat
     2281 gcattggaaa acttactgct gttgacgccg atggttaaag aatttctctt tttgccgccc
     2341 aaccagggtt cagtaaaaga aaaattatag gagcggaagg cacggccatt cgactgaacc
     2401 ctgagactaa gcttctgacc gtcgccggtt ggaagcggat cccaggagga tttcttccag
     2461 atatttcgga tagaaaagtt gttgaatgtc acacccagtg tacccgtcag accgatacca
     2521 ccaccccagc cggctgaaag ctctaactgg tcagatgatt tttcttctag tttccagtta
     2581 atgtccactg ttccatcttc tgcattgggc acaacacccg gatcaatggt ttcctggttg
     2641 aagaattgca ggttggccaa ttccctctgt gaacggatga ggtcagtacg actgaataat
     2701 tcaccaggaa tggtgcggag ttcacggcgg ataacataat cctttgtttt ctcgttacca
     2761 gcaatcgtca catttttgat ccttgcttgc ggaccttcag taattcgtat ttcataatca
     2821 atcgtatcat tataaacagc agtctctacc ggttcggcac ggaagaacag gtaaccgtca
     2881 tccatatagt agccactgat gtcgccacca ccatcttgtg aagcttcttt tcctaatcgt
     2941 ttattcagga tgctgatatt atatacatca cctttattaa tccccagtat aacattcagc
     3001 aatgaatcag aaaatttgga attacccttc caggcaatat tgccgaaata atacttgcgg
     3061 ccttcttcca ctttcaggtc aatattcagg tttccttttg gtgtggtata ctgtgtgtca
     3121 cctactatct gcgcatcccg gtaaccaagc gaattataat aatcaagtac cttttcttta
     3181 tcctcttcat atttctttgc atcaaatttt gctccgctga ataacttaaa ccggaaccat
     3241 gggtccaata aacgtttggt tttagttaaa gagagaaaac cccagtcctt tgtatactga
     3301 ctaaatgagt atttcttttt ctgaccatag ggactctctn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttc ccttcatctg ttttttcaac
     3421 cgtaatccat ctacactttc attaccaaaa aatgcaattt cgttgatacg tactttctta
     3481 cccttatcaa ctgtaattac catctggtta gaattcacaa aggatgaatc cgtaaactca
     3541 tcaatggtca cctttacatt gcggtaagct ttttctgtat agtatttggt aacgatctct
     3601 gtgatgcttc tgcgggtatt ttctgtaatg atagtttgtt tggccaatga tgcccgggtg
     3661 gttagttctt ctgcatctgt tttactgatg ccaacaaact tgaaatttcc cagccttggt
     3721 ctttcctgca catttacttc tacccatact ttatctcctt caacccgcgt aacgtatatc
     3781 tgaacatagg aaaagaattt ttgccgccaa aggttggcaa tggctttagc aaaaatatct
     3841 gcacccggat gaacgaactt atcgccgggt aggatgttag tgatagagct gataatggat
     3901 gtgtcgaggt gttttacacc tgtaatcttt acatcggcaa tagtatattc tttgggtacc
     3961 tgagcttttt cccactcaat aagtttagga tccaatgaag taggttttgt ggtatcctct
     4021 tgtgcctgta cagagaatac ggatacaaca gcagccgtca ataatgcaca aagcctcggt
     4081 agaaatcgtt gcatgtttga atgttctggt cttcttccct gctcgaaaca gggtttaatt
     4141 tgtttatttt ctgtgactta taaaataagc cgccaattta atcaggcggc aaattaacgg
     4201 taaatatcga aagtgtttgt taaaagaaac accggattat ggtgataata aagattttaa
     4261 ccttacggtc aggctttatc ctccacagtt acctgttcac tggttttacc aaaccttcgt
     4321 tccctgccct ggtaatcaat aatagcctca taaaggttat cctttctgaa gtcaggccag
     4381 cgaacattgg taaagtacaa ttctgcgtat gccagctggt aaagcaggaa attactgatc
     4441 ctgtattcgc cgctggttcg tatcatcagt tccgggtcgg gaaaaccgct ggtacagaga
     4501 taacgctgca acgtatcctg gttaatcgct tctacattca accggccact ttttacctcg
     4561 taagctatat ttttcactgc attcaacaac tcccatcgac cgctatagct taaagccatg
     4621 ataaggttga ggccggtatt cttccgggtt atttccaatg cttcgtccag ttctttcttt
     4681 gcgtaatcag gaagcatact catatcaccg attacatgca acctgatatt attcttattc
     4741 aggctttcca cttctttacg tatggtgttc accagtagtt ccatcaaacc cactacttca
     4801 tattcgggcc tgtcccagtt ctcagttgaa aaagcataaa gtgtcaggta ttcaaccccc
     4861 agttcggcac atccttctac aatgtttctg acactctcaa ccccatgaaa atgaccaaag
     4921 agacgatctt ctcctttttc ttttgcccag cgcccattcc catccataat aatagcaata
     4981 tggcggggca atctctgttt gtctatttgt tcaactaatg cggacatgcg gttttttatc
     5041 ggggcacaaa ggtaccatcg tttagccgta aatactaaaa gaaatgcccc cttgccggga
     5101 gcatttaatt aactggctca agttcaacaa ttacgatatt cttaatttct tccccaaatg
     5161 gccacccttg atttacccct tgcaaaacct cttgtttcat accagaattc aatctttgtc
     5221 aggtgccgta ggcctccgtc catatcaatc acccggcttt cgctaccctg ggcaaaccgg
     5281 ttgcgcaatg ccacttcctg tacacttccg ttgtcaaaat gaattttcat gttatacatt
     5341 ttcaaagggc catcggtaat ttttaattta atctggcgaa catcatcttt ccagtttcca
     5401 aagtggatta catcatgatc tactccgaag cctacattct tgtcgccaag aaaaaaccag
     5461 tcacccggtt taccggcaga ttctttgctt atagtagtaa aactaaaaag ggaaataaca
     5521 gacagcagta ataatgtttt tgtcaggcgc ataaagttta ttttaatggt tatgaat
//
LOCUS       DAORLS010000206         5547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_7986_length_5547_cov_1.987436, whole genome shotgun sequence.
ACCESSION   DAORLS010000206 DAORLS010000000
VERSION     DAORLS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7986_length_5547_cov_1.987436"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..161
                     /locus_tag="PK977_14005"
     CDS             6..161
                     /locus_tag="PK977_14005"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HRF19283.1"
                     /translation="MTAFSKFYPAENYHQQYFENNENNNPYCKVVIRPKLDKFRKVFK
                     DKLKTTN"
     assembly_gap    372..392
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<393..1511)
                     /locus_tag="PK977_14010"
     CDS             complement(<393..1511)
                     /locus_tag="PK977_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19284.1"
                     /translation="MRLTSTRIVFTFLFIVFSSLKSNSQDKYNFSGSSLVSGTALSVG
                     AVYKFTNVKSGVDATVRVEAITGGLTLTTIDGTGGFVEALQPTISVPPYSTGYVELLI
                     TFLVSGTATPSIQPEIPITPIDVDGQKYGGLPLYEFDEIQVTSGYTYFQYAGSELNMT
                     LNGTWARGKNKVSIDYPGIDTAQKQVMFTTVNPSVTSIRVRVGADNTSGSTASRLRSL
                     YFQKFNYPYTYVLEKEPSITFEGIIRNSMAELHGTITDAQQYNVQTVERSIDGIRFTE
                     IGNLSVTENNTTRYFSFFDATIPVGKIYYRIKLVNTVSKSEKFSSIFVLQNSNDQKRE
                     MTLINTIVNKYNPVLIIDSKNEETADISILDFNGQIKNH"
     gene            complement(1708..>1912)
                     /locus_tag="PK977_14015"
     CDS             complement(1708..>1912)
                     /locus_tag="PK977_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF19285.1"
                     /translation="KAFEKAIAETNDSLRYKLYQLADQIMMNDAPVVPLWYDKVVRLA
                     QPNVTGFKANALNLLELRYTKLK"
     assembly_gap    1913..1963
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1964..3367)
                     /locus_tag="PK977_14020"
     CDS             complement(<1964..3367)
                     /locus_tag="PK977_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF19286.1"
                     /translation="MTKMNVKCDRLLVALMLSTLFVLASCYNEKRSDKQIFHYNEFNG
                     IASLDPAFAKSQSTMWPAHQLFNTLVEIDDSLHIIPSLAKSWDISADKTVYLFHLRTD
                     VFFHDDPVFTNGKGRKMVAADVVYSLNRIIDKQVASPGSWIFNGKVDSIMPFLALNDS
                     TFQLRLLRPYNPILGIISMQYCSIVPKEAVDRYGTDFRRHPVGTGPFRFVDWEEGQAL
                     IFAKNEKYFETDSSGKRLPYLDGIKASFYDSKATEFLLFRQKQLDFINDIEASFKDEI
                     LTKQGTLRRDWEDKIVLQTSPYLNIEYLGILVDSANELVKNSPMRLRKIRQAINYGFD
                     RRKMVLYLRNSLGTPAESGFVPMGLPSFDTNAVKGYHYNPAKTKQLLAEAGYPGGKGL
                     PVVKLLTIAIYADMANFIAKQLEESGIPVQVEVIQKALLLTMTSSSTAAFFRGSWIAD
                     YPDAENYLSVFYSKNPAP"
     gene            3400..>4837
                     /locus_tag="PK977_14025"
     CDS             3400..>4837
                     /locus_tag="PK977_14025"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF19287.1"
                     /translation="MKNYLLQSVSIFVLTCFISFSSCAQTLNPKEKFTRQDTLRGSIT
                     AERAWWNVVHYNIQVAPDFRSKTIKGWNQIGFDVITGGKNKKMQIDLQYPMSIDSIIF
                     NKKRVRNFKRIGNAYIIDFGNINFMAAKTALQKRIPVTLHSVTIYFSGKPVEASRPPW
                     EGGWVWSKDAKGRPWMTVACQGLGASVWYPCKDHQSDEPDQGALLQVKVPDTLVAVAN
                     GRLYSKDDQLDGNMLYTWQVKNPINNYNIVPYIGKYVHFADNYIGETEKNLTLDYWVL
                     DYNLEKAKKQFGRDVKPMLKAFEYWFGPYPFYEDGFKLVESSHLGMEHQSAIAYGNYY
                     MDGYLGSDLSGSGWGLKWDYIIVHEAGHEWFANNITTNDIADMWVHEGFTMYSEVLFI
                     EYHWGKKAADEYLQGIRENINNDRPLIGPYGVNKEGSGDMYNKGAALLHTIRQVINDD
                     TVFRKILRGLNSDFYHRTVDSKDVEKYIS"
     assembly_gap    4838..4860
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4861..5115
                     /locus_tag="PK977_14030"
     CDS             <4861..5115
                     /locus_tag="PK977_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HRF19288.1"
                     /translation="LAKIFDQYLRTVNIPKLEFRVNGENLSFRWINCIKGFNMPVRFR
                     DSNNWLYPTTEWKTVAMTSDLIEKWITFDKNFYIRTARVN"
     gene            5147..>5547
                     /locus_tag="PK977_14035"
     CDS             5147..>5547
                     /locus_tag="PK977_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRF19289.1"
                     /translation="MSSIRRQSIISSMVIYIGFAVGLLNTYFFTKQGTFTEAEYGLTT
                     IFIAIATMMSAFATMAMPSYIFKFHPYYNDNLPPRKNDMITWALLVSLVGFILVMIAG
                     WTLKHLVIKKFGTNTPQLVAYYYWIFPLGLGL"
BASE COUNT         1668 a         1074 c         1071 g         1639 t
ORIGIN      
        1 cagaaatcac agcattttca aaattttacc cggcagaaaa ttatcaccag caatattttg
       61 agaataatga aaataataat ccatattgta aggttgtgat tcgtccaaaa ctggataaat
      121 tccggaaagt gtttaaggat aaactaaaga ccacaaatta attatcgtaa attctttctc
      181 ccgaaaaaat cgattaccat tatatccagt gggctaatta ctgaccaacg gaattactta
      241 actgattctt attgtacaat gaatttataa cttttattac ctgacttttt tctgataaca
      301 attacaaaat accccgactt caaaatagta cccggtataa ttacacgatt aatgccagta
      361 tgaaaattag cnnnnnnnnn nnnnnnnnnn nngtgatttt taatttgtcc gttaaagtct
      421 aaaatactga tatcggcggt ttcttcattt ttactgtcta ttattaatac cggattatat
      481 ttattcacaa tagtgtttat caaagtcatt tctcttttct gatcattact gttttgtaat
      541 acaaaaatgg aactgaattt ttcggactta ctgacagtat tcaccaattt tattctataa
      601 tatatttttc ctacaggaat tgttgcatca aaaaatgaaa aatacctggt agtattattt
      661 tcagtaacag atagatttcc aatttcggta aaccttattc catcaatact tctttcaact
      721 gtctggacat tgtattgctg ggcatcagtt attgtaccat gcaattcagc cattgagttt
      781 ctgattatcc cttcaaaggt tattgaaggt tccttttcca aaacataagt gtagggataa
      841 ttgaatttct gaaaatacag gctacgtaac cgggatgcag tagaaccaga tgtattatcc
      901 gcacctaccc tcactcttat tgaagttaca cttggattaa cagtagtaaa catcacctgt
      961 ttctgagcag tatcaatacc cggatagtca atggaaactt tgtttttacc ccttgcccag
     1021 gttccattta gtgtcatatt caattcactt ccagcatatt ggaaataggt gtagccgcta
     1081 gttacctgga tctcatcaaa ctcataaaga ggtaaacctc catatttttg gccgtctaca
     1141 tcaataggag tgatgggaat ctcgggttga atggagggag tggccgtacc tgaaacaaga
     1201 aatgtaatta acagctcaac atatccagtg ctgtatggcg gaacagaaat tgttggttgc
     1261 aacgcttcta caaatccgcc ggtgccatca atcgtagtaa gcgttaatcc tccggttatt
     1321 gcttcaaccc taaccgttgc atcaacacct gatttaacgt ttgtgaattt atacacggct
     1381 ccaacagaaa gtgcggtgcc actaacaagg ctgctaccgg aaaaattata cttatcctga
     1441 gaattacttt taagtgaaga aaagacaata aataaaaaag taaatacaat ccgggtagat
     1501 gtaagtctca tagtacggct tttttgtttt acaaagggtt gagatcagtt ggcaattcta
     1561 aggagactgg attttttctt aagaaaaaat cccaaacatt tattcggatt ttttctgcca
     1621 ccaaaagtaa tatcccggtt taaaatatac aatcggaaaa gtactaatcc gataaatatt
     1681 tagtagaaat actatggttt ttaaatttta tttcagcttg gtatatctta attccagcag
     1741 gttcagtgca ttggctttaa aacctgtcac attgggctgt gccaatctaa ccactttgtc
     1801 ataccacaaa gggactacag gagcatcatt catcataatt tgatcggcca gctgataaag
     1861 tttgtaacga agagaatcat ttgtttcagc aattgctttt tcaaaagctt tgnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggggcg ggattcttgg
     1981 aataaaaaac agacaaataa ttctctgcat ccgggtaatc tgctatccag ctgccccgaa
     2041 agaaggcagc agtagaactg gacgtcatgg ttaataacaa tgctttttgt attacctcaa
     2101 cctgtaccgg gatacccgac tcttccaact gcttggcaat aaaattggcc atatcagcat
     2161 aaatagcaat ggttagcagc tttacaaccg gcaacccttt gcccccgggg taaccggctt
     2221 cagccagcaa ctgttttgtc ttagccggat tataatgata tccctttact gcattggtat
     2281 caaatgaggg taatcccatt ggcacaaagc ctgattcggc aggtgtacct aaagaattgc
     2341 gcaggtacaa caccattttt cgcctgtcaa atccatagtt gattgcctgt ctgatttttc
     2401 gcagccgcat aggcgaattc ttcaccagtt cattggcgga gtcaactagt atgcccaggt
     2461 attcaatatt gaggtaaggg cttgtttgca gcacaatctt atcttcccaa tcccttctaa
     2521 gagttccttg tttcgttaat atttcatcct taaacgatgc ttcgatatcg ttgataaagt
     2581 cgagttgctt ttgcctgaat aataaaaatt ctgttgcctt gctgtcataa aatgaagcct
     2641 tgataccatc caggtaaggc aatctttttc ctgaactatc tgtttcgaag tatttttcat
     2701 tttttgcaaa aatcagggcc tgtccttctt cccagtccac aaagcgaaaa gggcctgtgc
     2761 cgaccgggtg cctgcgaaaa tcagttccat atctatctac tgcttcttta ggcactatag
     2821 aacaatactg catggatata atgccaagga tcggattgta tggtcttaat aatctcagct
     2881 ggaaagtaga gtcattaaga gcaaggaaag gcataatgga atctactttg ccattgaaaa
     2941 tccatgagcc gggactggct acctgtttat caattatccg gttgaggcta tacactacat
     3001 cagctgccac catttttctg cccttgccgt tagtgaagac aggatcatca tgaaaaaaaa
     3061 catctgttct taaatgaaac aggtagacgg ttttatcagc cgaaatatcc cagctttttg
     3121 ccaaagaagg aataatatgc aggctgtcgt caatttcgac caatgtattg aataactggt
     3181 gagccggcca catggttgac tggcttttgg caaacgccgg gtcaagagaa gcaataccat
     3241 taaattcgtt gtaatgaaaa atctgtttat cgcttctttt ttcgttatag caggaggcca
     3301 atacaaaaag agttgacagc atcagagcaa caagcaaccg gtcacattta acgttcatct
     3361 tggtcattgg gccttaaatt tacagtaaca atggatacta tgaagaatta cctgttgcaa
     3421 tcagtttcaa tatttgtttt aacctgtttt atttcttttt cgtcttgtgc acagacattg
     3481 aatccaaaag aaaagttcac acggcaggat acattgcgtg gcagcatcac agcggaaagg
     3541 gcttggtgga atgtggtaca ttataatata caggttgccc ctgatttcag gtcaaagacc
     3601 attaaaggct ggaatcaaat cggtttcgat gtaataactg gcggtaagaa taagaaaatg
     3661 cagatagacc tgcaatatcc gatgagtatt gacagcatta tatttaataa gaagagggta
     3721 aggaatttca agaggatcgg gaacgcctat atcattgact ttggtaatat taacttcatg
     3781 gcagcaaaaa ctgcgttaca aaaaaggata ccggtcacac ttcatagtgt cacgatatat
     3841 tttagcggta aaccagtaga agcttcccgt ccgccttggg aaggaggctg ggtttggtca
     3901 aaagatgcaa aaggccgccc ctggatgaca gtagcctgcc agggattggg tgccagcgtt
     3961 tggtatccct gtaaagacca tcaaagcgat gaaccagacc agggtgcttt gttgcaggta
     4021 aaagtaccgg ataccttagt ggctgttgcc aatggccgtt tgtattcgaa ggatgatcag
     4081 cttgacggca acatgctata tacctggcag gtaaaaaatc ccatcaacaa ttataacata
     4141 gtaccctaca taggcaaata tgtacacttc gctgataatt acattgggga aacagaaaag
     4201 aacctgacac ttgattattg ggtacttgat tacaacctgg aaaaagcaaa aaaacaattt
     4261 ggtcgcgatg taaaacctat gctgaaagca tttgagtatt ggtttgggcc ttatccattt
     4321 tatgaagatg gttttaaact ggtcgaatca tctcatcttg gcatggaaca ccaaagtgct
     4381 attgcctatg gtaattatta tatggatgga tatctcggat ccgatttgtc cggaagtggc
     4441 tgggggttaa aatgggatta catcatcgtt catgaagcgg gacatgagtg gtttgccaat
     4501 aatatcacta ccaatgatat tgcagatatg tgggtgcatg aaggcttcac tatgtattcc
     4561 gaagtactgt tcatcgaata ccactggggt aaaaaagcag ccgatgaata cctgcaggga
     4621 atccgggaaa atattaataa tgacagacct cttattggcc catacggtgt aaataaagaa
     4681 ggtagtggtg atatgtataa caaaggcgcc gccttgctgc ataccatcag gcaggtaatt
     4741 aatgatgata cagtcttcag aaagatatta agaggattga acagcgattt ctatcatcgc
     4801 actgtcgatt caaaagatgt tgagaaatat atttcagnnn nnnnnnnnnn nnnnnnnnnn
     4861 cttgcaaaga tttttgatca gtatctccgt accgtcaata tacccaaact ggaatttaga
     4921 gttaacgggg aaaacttatc attccggtgg atcaactgta taaaaggttt caatatgcct
     4981 gttcgattcc gggattccaa taactggcta tatcctacta cagaatggaa aactgtggct
     5041 atgacctctg atctgataga aaagtggatt acgttcgaca agaactttta cattagaact
     5101 gccagggtga actgacaatt accaaatcgc ctcctatctt taccacatga gcagtatccg
     5161 ccgacaaagc atcatttcat ccatggtcat ttatattggc tttgctgtcg gtttattaaa
     5221 tacctatttc tttacaaaac agggcacttt tacagaggca gaatatggac taactaccat
     5281 tttcatagct attgccacca tgatgtcggc cttcgccact atggccatgc catcttatat
     5341 ttttaaattt catccttact acaatgataa cctgccgccc cgaaagaatg atatgatcac
     5401 ctgggcactg ctggttagcc ttgttggttt tattttagtt atgattgccg gctggacgct
     5461 gaaacacctg gtcataaaaa aattcggtac caatacgccg caactggtag cttactatta
     5521 ttggattttc ccactgggcc ttggact
//
LOCUS       DAORLS010000207         5517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8051_length_5517_cov_1.948371, whole genome shotgun sequence.
ACCESSION   DAORLS010000207 DAORLS010000000
VERSION     DAORLS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8051_length_5517_cov_1.948371"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK977_14040"
     CDS             complement(<1..215)
                     /locus_tag="PK977_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRF19290.1"
                     /translation="MGKTLFEKIWEKHIVKTIDGGPSVLYIDKHFIHEVTSPQAFTGL
                     IKRGIKVFRPKQVVATADHNVPTLNQHL"
     assembly_gap    329..449
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<450..1410)
                     /locus_tag="PK977_14045"
     CDS             complement(<450..1410)
                     /locus_tag="PK977_14045"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRF19291.1"
                     /translation="MTGNKVFIFDTTLRDGEQVPGCKLNTKEKLKLALKLEALGVDII
                     EAGFPISSPGDFESVNQIARHVKNVTVCGLTRAVQKDIDVAAQALKYAVRPRIHTGIG
                     TSDLHIKAKFNSTREEILERAVQCVKWARHYTDDVEFYAEDAGRTDNDYLARVIEAVI
                     KAGATTVNIPDTTGYCLPSQYGEKIAYLVNNVPNIDKAVISCHCHNDLGLATANSIAG
                     VIAGARQIECTINGLGERAGNTSLEEVAMIIRQHKDLGLYTTIQAEQLNPISRLVSDT
                     MRMPVQPNKAIVGSNAFSHSSGIHQDGFLKNAQTYEIIAPEEVG"
     gene            1667..>2043
                     /locus_tag="PK977_14050"
     CDS             1667..>2043
                     /locus_tag="PK977_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19292.1"
                     /translation="MPNRSTDSLFQLIKSLEKSEKRNFKLYVKRNTDTADLKIIQLFD
                     ALDKMNDYDEAQLLKKAKGIKKQQLSNIKAHLYRQILSSLRLIKEEDNIDIRLHEQMD
                     HARILYNKGLYLQSLKVLDKLKDT"
     assembly_gap    2044..2094
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2095..3223
                     /locus_tag="PK977_14055"
     CDS             <2095..3223
                     /locus_tag="PK977_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19293.1"
                     /translation="FEKKIETLYITRSMQDRADKLAAEADAVHAQLTLVNKLSNLSLQ
                     LYSWYIQHGHARNEKDIKSITAFFEKQLPADARQAKGFYEKLYQYQAYVWYAFIRQDF
                     LQYYRYSQKWVDLFAAYPGLLRVETASYIKGMHNLMGAQFNLFRHEKLANTIRQFEQF
                     VKQKHLAENENNRILAYQYLYTARINLYFLRGTFTKGLRLVPFLEDMLQQYSLYLDTH
                     RVLVFYYKIASLYFGSGDNEKAIDYLHRIIHQKGDLRTDLQCYARLLHLIAHYELGNF
                     DLLEYLIKSVYRYMAKLENLSKVEEEMFAFLRRSFKVGAAALKPEFEKLLAKLKKYEG
                     NPLEARAFAYLDVISWLESKISGVQVQDVIRQKFLLGKRGR"
     assembly_gap    5184..5266
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5267..5474
                     /locus_tag="PK977_14060"
     CDS             <5267..5474
                     /locus_tag="PK977_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19294.1"
                     /translation="YAMTYIVNPKNKKEAKVLETILKGLSIGFYSEEEEDAAILHAME
                     KGRKSRLLTAEEKKSFLKKLKSAK"
BASE COUNT         1463 a         1170 c         1165 g         1464 t
ORIGIN      
        1 aggtgctgat tgagggtagg cacattgtga tcggcagtgg ccaccacctg tttcggacga
       61 aatactttga tacctctttt aatcaatcct gtaaaagcct gcggactcgt tacttcatga
      121 ataaagtgtt tgtcgatata taaaacagaa ggcccgccgt caattgtttt gacgatatgc
      181 ttctcccaga ttttttcaaa tagtgtcttg cccattttct tatgctactg ctgctttgga
      241 ttgatattgt ttagcaatga atgcaagatc gccatcctcc acttctttct tgctgtcagc
      301 tacttttaaa aattcatcat ataatacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccaacctct tccggtgcaa tgatctcgta
      481 ggtttgtgca tttttcaaaa atccatcctg gtgaatgccg gaggaatgag aaaacgcatt
      541 gctgcccaca atcgctttat tcggttgcac cggcatccgc atagtatcgg ataccaggcg
      601 gctgatcggg tttagttgtt ccgcttgtat ggtggtgtat aatccgaggt ctttgtgctg
      661 cctgatgatc atggccactt cttccagtga agtattgcct gctctttcac ccagcccgtt
      721 aatggtacat tcaatctgcc gggcaccggc tattacaccg gcaatagaat tagcagtggc
      781 cagcccgaga tcattatgac aatgacagga aatgacagct ttgtcaatat tcggaacatt
      841 attgactaag tatgcgatct tctccccgta ttgcgaaggc aggcagtagc ctgttgtgtc
      901 aggaatgttc actgttgtgg ccccggcttt tatcacggct tctattactc ttgccaggta
      961 gtcattgtca gtgcggcccg catcttcggc ataaaactcc acatcgtcgg tataatgccg
     1021 ggcccatttt acacactgta cagccctttc taaaatttct tctcttgttg aattgaattt
     1081 tgctttgatg tgcaggtcag atgtaccgat acccgtatgt atcctcggcc ttacggcata
     1141 cttcaatgcc tgcgctgcta catcaatatc tttttgtacg gcacgggtca gtccgcatac
     1201 cgttacattt tttacatgcc gggctatctg gttcaccgat tcaaaatcgc cgggagagga
     1261 aataggaaat cctgcttcta taatatctac tcccagtgct tccagtttca gcgccagttt
     1321 cagtttctct tttgtattca gtttgcagcc cggcacctgt tcgccgtcgc ggagggtagt
     1381 gtcgaatata aaaaccttgt tgcctgtcat actatttaaa agattagtgt gggtataaaa
     1441 atgcaggctg cttatttttg taccagtccc ggcacattac ttggaaaaat attgttggag
     1501 acggctattg cttgctataa ttcattcaat aaacaacctg cgcaactaag ttacctttag
     1561 gttaacggca ggagaaaaca aactgtggcg gattttacac cccgtaaaga ggctgttttc
     1621 cgctgaaacc cttaccggta ctgactttta aacttttttc attacgatgc ccaaccgctc
     1681 cacggatagt ctttttcagc tcatcaagtc actcgaaaag agtgaaaagc ggaatttcaa
     1741 gctgtacgta aaacgcaaca ccgatacggc cgatctcaag atcatccagt tgtttgatgc
     1801 gctggacaag atgaatgact acgacgaggc ccagctgctc aaaaaagcaa agggcatcaa
     1861 aaagcagcag ttgtccaata tcaaggcgca cctgtaccgg cagatccttt ccagcctgcg
     1921 cctcatcaag gaagaagata atattgatat ccgcctgcat gagcagatgg accatgcccg
     1981 catactatat aataaaggcc tctacctgca aagcctgaag gtactggaca aactaaaaga
     2041 cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctttga
     2101 aaaaaaaatc gagacactct acatcacccg cagcatgcag gacagggccg acaaactggc
     2161 ggcagaggcc gatgctgtgc atgcacagct tacccttgtc aacaagctgt ccaatctttc
     2221 gttgcagctc tacagttggt acatacagca tggccatgca cggaatgaaa aagacatcaa
     2281 aagcatcact gccttttttg aaaaacaatt acctgcggat gcccggcagg ccaaaggctt
     2341 ttatgaaaag ctttaccagt accaggccta tgtgtggtac gcttttatcc ggcaggattt
     2401 tttgcagtac taccgctatt cgcaaaaatg ggttgatctg tttgctgcct atcccggcct
     2461 gctgcgggta gaaacagcca gctatatcaa gggcatgcac aacctgatgg gggcgcagtt
     2521 caacctgttc aggcatgaaa agctggccaa taccatccgc cagtttgaac agtttgtaaa
     2581 gcaaaagcac ctggccgaaa atgagaacaa ccgcatactg gcttaccagt atctctatac
     2641 ggcccgtatc aatttgtatt ttctccgcgg cacttttacc aaggggctgc gccttgttcc
     2701 ttttttggaa gatatgcttc agcagtacag cctctacctt gacacacacc gggtgctggt
     2761 attctattac aagatcgctt cgttgtattt cggcagtggt gacaatgaaa aagccattga
     2821 ttacctccac cgcatcatcc accagaaggg cgacctgcgt accgacctgc aatgctatgc
     2881 ccggctgctg cacctcattg cccattatga gctgggcaat tttgacctgc tcgaatacct
     2941 cattaaatcg gtgtaccgct acatggccaa attggaaaac ctgagtaagg tggaggagga
     3001 gatgtttgcc ttcctgcgcc gctcctttaa ggtgggggca gccgcattga aacccgagtt
     3061 tgaaaaactg ctggccaaac taaagaaata cgaaggcaac ccgctggagg caagggcctt
     3121 tgcctacctg gatgtgatct cctggttaga gagcaagatc agcggtgtgc aggtgcagga
     3181 tgtgatcagg caaaaatttt tgttggggaa aagggggagg tgacgagcat ggtgtataaa
     3241 cctgctgcgc tcttcccggc ggccggcaat ggcttcccgg agatgatttt tgtgtttcct
     3301 gcgaccgaca gcatcgttcc gtacgccgac aattgacatc ccggcagttt cttttgtcat
     3361 cccggcagtg ccaaaaccgt tccggagagt gacagcggca gtcccgaagg tgacaatggg
     3421 cttccggatg ctgacaattg tcatcccggc agcgacaatg gctttccgga cgctgacaat
     3481 tgccatcccg gcagtgacaa aaccgttccg gacgctaaca atggccgtcc cgaagggtgc
     3541 aacggacttt ccgtcaatgc attatacagt tccgaccctg aaaatgagga aaacaaagta
     3601 aagggaagag aaagttgaga caatatctaa aaagtaatgc tggcaagggt tctaccttag
     3661 aaaaagtttt aaagttttgt tgtggagtga tttcccacaa aggcacaaag gacacgaaga
     3721 aaagatttaa ccacatctgt ctaccagcct ttttgtctga acctggattg ggggagatta
     3781 ttaggattgt gggatgtgtc attccggcct tgagccggaa tctttttctt ttgttgatag
     3841 ccggtggcgc aagatatcta attgaaatga gcagtaatgc ttcgcaggcg acaagtccct
     3901 ctactggttt tctttaagtt gaatggcgta actgaggaga ctagtcgggg aaggaaataa
     3961 gcggatgatt aaaaaagaaa gtaaaaaata agaaacagta ttaatgtttc cacaatccaa
     4021 atccaaagaa ttcgctcttt taatactagt cgaatgatta ggtagaaagt ccccagtata
     4081 aagaaggccg gaataaacat acctccaatt tgattagtcc acctttcagt atggtaaggg
     4141 tcgttacgga agaatagtcc tataaaaaat gtaagagcta tcacgacgct gaaaggtgac
     4201 aggtagtaaa agaaattttt aaagaattga gcagtggtca taaattggaa atcaatgtct
     4261 ctcagttgcg cacaacgttc gagtatttgc gaaggcaggg aattcattga ttgtccagcc
     4321 cggtacaaat gcccatttga aaatatgttg ttgaagttaa gaactaaagc tgaacattga
     4381 acgtcgagcc cgaaccgctg ctgatgcttg cacatagctg atgttacaat gtccagccct
     4441 gcttttgcaa atacttttgt tggtggcagt ttttctatgt tcaaataatc cgacaatcca
     4501 actacaattt ctcttcacta acttggtcag gctctcctgg ataagttccc ttatttaatg
     4561 tcaaaggcat ttctgtctgt tgatgattat aatctgctat tatttttaat ggcacgggtt
     4621 ggtttgtcga aactttaaat tttgcaatcc atagaaatcc tttttgcttg aaatttatct
     4681 tctttggaat accattgtca gaataggtac agtagattaa agcatgctta agtttgagtc
     4741 tccctttact attaccgaca ctgtcctgtt gttgtaatga aactcggata gttatgaatg
     4801 ctgtcttttt tcgtacttag tctttcaaca ccgtatgccc aatagcattt tgcacttggg
     4861 aatgatttca gtgattcagg tacaggaact tcttgagccg ttgcaatgtc agaaatacac
     4921 aaaaaaaaga ttaagcatat aagaagccct ttcagttgtt cacctctttg tcttagggtt
     4981 actttcgata tattctttct aaccatgtaa tatagggtgt gtcgcacaaa ttgccaccaa
     5041 cacataaatt tacgccgtag gaattcaatt ttcaaaaatt acaagtattg aaaatcaata
     5101 tttttatctt acgttttttg tacctgcctt ccggcaggca ggttgttacg gcgtcaatac
     5161 aaagatcaat taaataaccg cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttat gctatgacat
     5281 acatcgtaaa tccaaagaat aaaaaagaag ccaaagtgct ggagaccata ctcaagggtt
     5341 tatccatcgg attttactca gaagaagaag aggatgctgc catcttacat gcaatggaaa
     5401 aaggcaggaa gagccgtttg cttacggcag aagagaaaaa atcttttctt aagaaactga
     5461 aatcagcgaa atgaaaattg aaatccgcaa gtcgtttgag aaagatgcgg ataaatt
//
LOCUS       DAORLS010000208         5500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8082_length_5500_cov_2.223691, whole genome shotgun sequence.
ACCESSION   DAORLS010000208 DAORLS010000000
VERSION     DAORLS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8082_length_5500_cov_2.223691"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>215
                     /locus_tag="PK977_14065"
     CDS             17..>215
                     /locus_tag="PK977_14065"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012687.2"
                     /note="catalyzes the formation of
                     3-(imidazol-4-yl)-2-oxopropyl phosphate from
                     D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and
                     histidinol from histidinol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HRF19295.1"
                     /translation="MLEQLSRHSNVDISLKVEGDLHIDEHHTIEDTAITLGEAFAKAL
                     GNKKGIERYGFTLPMDDCLAQV"
     assembly_gap    216..225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <226..485
                     /locus_tag="PK977_14070"
     CDS             <226..485
                     /locus_tag="PK977_14070"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582714.1"
                     /note="catalyzes the formation of
                     3-(imidazol-4-yl)-2-oxopropyl phosphate from
                     D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and
                     histidinol from histidinol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HRF19296.1"
                     /translation="FGGRSWLVWHAAFKREKIGDMPTEMFYHFFKSFSDAAKCNLYIK
                     AEGENEHHKIESIYKALAKAIKASIRRDPDNQQLPSTKGIL"
     assembly_gap    558..596
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <597..1088
                     /gene="hisH"
                     /locus_tag="PK977_14075"
     CDS             <597..1088
                     /gene="hisH"
                     /locus_tag="PK977_14075"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRF19297.1"
                     /translation="QIITSDKVIFPGVGEASTAMKYLSERNLDKVISSLHQPVLGICL
                     GMQLMCKHSEENNTPCLGIFEDVKVKKFDRAKKNLKVPQIGWNNITGLKTALFKSIEA
                     NSYCYYVHGYYAELSKHTIATSDYGKLYSAALNKDNFFGVQFHPEKSAAIGEIILKNF
                     LAL"
     gene            1085..1852
                     /gene="hisA"
                     /locus_tag="PK977_14080"
     CDS             1085..1852
                     /gene="hisA"
                     /locus_tag="PK977_14080"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRF19298.1"
                     /translation="MTAPLKTMQKKEAIKSYGFIIPAIDIIDGKCVRLTKGDYNLKKI
                     YNEDPLEIAMQFEDTGLKRLHLVDLDGAKAGEVKNWKVLECIAGKTNLVIDFSGGISS
                     QKSAEITFNSGAAFASVGSIAIKDEIAFTSWLLKFGPEKFIIGADVLDEKIVIGGWTE
                     KTPVNVFELIGQYKTKGIKQFFCTDVSKDGMLGGSAISLYKKILNEHPSIDLIASGGV
                     SKIKDIEELREAGCEGVIIGKAIYENIISLNQLKRFC"
     gene            1852..2607
                     /gene="hisF"
                     /locus_tag="PK977_14085"
     CDS             1852..2607
                     /gene="hisF"
                     /locus_tag="PK977_14085"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRF19299.1"
                     /translation="MLTKRIIPCLDIKDNRTVKGTNFLQLRDAGDPVELAVRYCEEGA
                     DELVFLDITATTEKRKTLAKLVKEISVNINIPFTVGGGINSISDVSVLLENGADKVTI
                     NSSAVKNPSLINDLAKNFGSQCVVLAIDAKIINDEWRVVTHGGRNPTNLSVQAWAKES
                     ADRGAGEILLTSMENDGTKRGYATELTRQIAEVAPVPVIASGGAGKMEDFAIVFEAGK
                     ADAALAASIFHFKEISIPSLKKYLADKKIPVRI"
     gene            2604..3194
                     /gene="hisIE"
                     /locus_tag="PK977_14090"
     CDS             2604..3194
                     /gene="hisIE"
                     /locus_tag="PK977_14090"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRF19300.1"
                     /translation="MKPDFSKYTDALLPVIIQDQYSNKVLMLGFMNEEAWNKTNIEKK
                     VTFYSRSKNRLWTKGETSGNYLFVKEILTDCDNDTVLIKVYPAGPVCHTGNDTCFNEI
                     NTSFTLQKLEEIINERKINPSENSYTSSLFGKGINKIAQKVGEEAVELVIESKDDDKE
                     KFLGEAADLLFHYLVLLQAKGFTLKDVENVLASRHK"
     gene            complement(3252..4676)
                     /locus_tag="PK977_14095"
     CDS             complement(3252..4676)
                     /locus_tag="PK977_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19301.1"
                     /translation="MRKCLYIEIILVLVSGLCFGQTGFRNSGNLQLHTGTNMAVVGNV
                     ENSSTANLINNGSLYLKGNLTNHQALMSAGTGTLYLNGTSIQTIAGTELIKTSDLVTD
                     NIAGIVLNNNLTVSGTHTFTNGIITTSATPNYLIYEAVATHNGSADNRHISGWVKKIG
                     TSDFVFPVGNGTYLRPVAMTNLSGSAEFNCHYFNTTNNIYELWSPLVQVKSNEYWQVD
                     KISGGTAQITLNWDNSKVPMDNIDLADILSAEYNGNDWESKGGSATGDVSTTGSVTSN
                     NINTFGEMTLGYKSFPVPLKLISFSGLRRNGTSFLDWTTDNEFNVSHFEVQRSYDASS
                     FTTIGNRTARNSGSRELYSFEDPVFFRGIVYYRLRSVDNDGTFSYSKIIALSEKDIIS
                     SDFVVLNPARYAITIFNKTTEQGVFGYRLINSGGQVILKGNVNFELNGNAVIQLPSQT
                     ASGTYMLELRNSNILFTRQVLVER"
     gene            complement(4699..>5500)
                     /locus_tag="PK977_14100"
     CDS             complement(4699..>5500)
                     /locus_tag="PK977_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19302.1"
                     /translation="NAEIWVFETDISNSQGSGILVHSNVSGVKVKVAETDFIGCNYGI
                     NLSKGSAATIQLASGGYYNALSGDTAIYYQPTTFTLPTNISITGNSWNNIGKYIEGFD
                     FTRSDGRDKDVILESNAGMGDKKPSCYINVLNGSTTTTLTTANSWYKANWTNTASSTA
                     KWTIANNRITYQPTNKRDGVIVITGNLSVNNSSRTISFALVKNGVTSTRYGETTLRTS
                     SAGTASQFSTVIYISDISSTDYFEVYCSSANSGDIITILDLNWQVTTQ"
BASE COUNT         1785 a         1010 c         1093 g         1563 t
ORIGIN      
        1 gaattttttt aatcacatgc tggaacaatt aagccgtcac agcaacgttg atatctcact
       61 aaaagtggaa ggtgatctgc atattgatga acaccatacc atagaagata cagccattac
      121 ccttggcgaa gcatttgcaa aagctcttgg taataaaaaa gggattgagc gatatggctt
      181 cacactgcct atggatgatt gcctggcaca ggtagnnnnn nnnnnatttt ggcggaagaa
      241 gctggctggt atggcatgca gcttttaaaa gagaaaagat tggagacatg ccaacagaaa
      301 tgttttatca tttttttaaa tcattttctg atgcagcaaa gtgcaacctg tatataaaag
      361 cagaaggaga aaacgaacat cacaaaatcg aatccatcta taaagcactg gcgaaagcaa
      421 taaaagcttc tattagaaga gatccggata atcagcaact gccaagtaca aaaggaatac
      481 tttaatgaaa gtagtagtta ttaaatataa tgcgggtaat atacagtcag tcctctttgg
      541 attggagaga attggcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaaa
      601 ttattacttc tgataaagtc atcttcccgg gagtcggtga agccagtacg gcaatgaagt
      661 atctgtcaga aagaaatctt gacaaagtca taagttcact tcatcagcct gtattgggaa
      721 tatgtcttgg aatgcaacta atgtgtaaac attcagaaga aaacaatact ccctgcctgg
      781 gaatatttga agatgtgaaa gtgaagaaat ttgatcgtgc aaaaaagaat ttaaaggttc
      841 cccaaatcgg atggaataac atcaccggcc taaaaacagc tttgtttaaa tctattgaag
      901 caaacagtta ttgctattat gtgcacggct attatgcaga acttagcaaa cacaccattg
      961 caacttcaga ttatggaaaa ctgtacagtg ctgccttgaa taaagacaat ttctttggag
     1021 tgcagttcca ccccgagaaa tccgcagcaa tcggtgagat aatactcaaa aactttttag
     1081 ccctatgaca gcacctttaa aaacaatgca aaaaaaggaa gccataaaga gttatggctt
     1141 tataataccg gccattgaca tcattgatgg taaatgtgtt aggttaacaa aaggcgatta
     1201 taacctaaag aaaatataca atgaagatcc gctggaaatt gctatgcaat ttgaagatac
     1261 cgggcttaag cggttgcatt tggtagactt agacggggct aaggccggtg aggttaaaaa
     1321 ctggaaagta cttgaatgta ttgccggcaa aacaaacctt gtgatagact ttagcggagg
     1381 catcagttca cagaaaagcg cagaaatcac tttcaactca ggtgctgcat ttgcttccgt
     1441 tggcagtatt gctataaaag acgaaatagc ctttaccagc tggttgctga aatttgggcc
     1501 tgaaaaattt attattggag cagatgtatt agacgaaaaa attgttatcg ggggctggac
     1561 agagaaaaca ccggtaaacg tttttgaatt aatcggccag tacaaaacta aagggataaa
     1621 gcagtttttc tgtacagatg taagcaaaga tggtatgctg ggaggatcgg ccatctcgct
     1681 atacaaaaag atactaaacg aacatccttc tatcgattta attgccagtg gcggagtgag
     1741 taagataaaa gatattgaag agttaagaga agcaggttgt gaaggtgtaa ttatcggcaa
     1801 ggcgatttat gaaaatataa tttcgttgaa ccaactgaaa cgattttgct gatgctgacc
     1861 aaaagaataa tcccatgtct tgatataaaa gataacagga ccgtaaaggg aactaatttt
     1921 cttcaactgc gtgatgccgg tgacccggta gaattggctg taaggtattg tgaagaagga
     1981 gctgacgaac tggttttttt agacattaca gctacaactg agaaaaggaa gacattagca
     2041 aagttggtaa aggaaatctc agtgaatata aatattccct tcactgtagg gggtggcatc
     2101 aacagtattt cagatgtgag tgtattgctg gaaaatgggg cagataaggt aacaataaac
     2161 tcatctgctg tcaaaaaccc ttcgctgatc aatgatttgg caaaaaactt cggcagtcaa
     2221 tgtgtggttt tggctattga tgctaagatt attaatgatg agtggagggt ggttacccac
     2281 ggtggaagaa atcctacaaa tttatctgta caggcttggg caaaagaaag tgcagacaga
     2341 ggtgccgggg aaattttact cacttccatg gaaaacgatg gaaccaaaag aggctatgca
     2401 acagaactta caaggcaaat tgcagaagtt gctcctgtac ctgttattgc ttcgggcggt
     2461 gccggtaaaa tggaagactt tgcgattgtt tttgaagcag gaaaagctga tgcagccctt
     2521 gctgccagta tttttcattt caaagagatt agcataccca gtttgaaaaa atatttagct
     2581 gacaaaaaaa tacctgtaag aatatgaagc ctgatttttc caaatatacc gatgcactcc
     2641 taccagttat tatccaggat cagtattcaa acaaagtgct tatgcttggt tttatgaacg
     2701 aagaagcctg gaataaaaca aacatcgaaa aaaaggttac tttttacagc cggagcaaga
     2761 accggctttg gaccaaaggc gaaacttccg gcaattatct ttttgtcaaa gaaatactga
     2821 cagactgcga taatgataca gttcttataa aagtataccc cgcaggcccg gtctgccata
     2881 ccggcaatga cacctgtttc aatgaaataa acacttcttt tactttacag aaacttgaag
     2941 agattattaa tgaacgtaaa ataaatccat ctgaaaattc ttacacctcc tcattatttg
     3001 gtaagggcat taacaaaata gcccagaaag tcggagaaga agctgttgaa ctggtaattg
     3061 aaagcaagga cgatgataaa gaaaaattcc tgggagaagc tgcagatctt ctttttcatt
     3121 acctcgtttt gttgcaggcc aaaggcttca cattaaaaga tgttgaaaat gtattggcga
     3181 gccggcataa ataataccgc cccgtttccg gggcggtcgc tcagggttcg tattaatcta
     3241 ctaactaata atcatctttc aaccaatacc tgtcttgtaa ataatatatt actatttctt
     3301 aattcaagca tataagtacc agaagctgtt tgtgatggca attgaatcac tgcattccca
     3361 tttaactcaa aatttacgtt acctttaaga atcacctgcc ctcctgaatt aattagccga
     3421 taaccaaaca caccttgttc tgttgtttta ttaaaaattg taatcgcata ccgtgccggg
     3481 ttaagcacta caaaatcaga actgataata tctttttcgc taagtgcaat tatttttgaa
     3541 tagctgaatg tgccgtcatt atctacactg cgaagcctgt aataaacaat accacgaaaa
     3601 aaaaccggat cttcgaagct atataactcc ctgctaccac tattcctggc cgtcctatta
     3661 ccaatggtag tgaaggaaga tgcgtcataa cttctttgta cttcaaagtg gctaacattg
     3721 aattcattat cggtagtcca atcaagaaaa ctggttccat ttcgccttaa tccggagaat
     3781 gagataagtt ttaaagggac aggaaatgat ttatacccca atgtcatttc accaaatgta
     3841 ttgatattgt tagaggtaac actaccagtt gttgaaacat cacctgtagc agaaccaccc
     3901 ttgctctccc agtcattgcc attatattca gcagataaaa tatcggccag atctatgtta
     3961 tccattggca cttttgaatt atcccagtta agtgtaatct gtgcagtacc accgcttatt
     4021 ttatcaacct gccagtattc attggacttt acctgtacaa gcggtgacca aagttcataa
     4081 atattatttg tcgtgttaaa gtaatgacaa ttaaactcag cgctaccgga aagattagtc
     4141 atagcaaccg gccttaaata tgtaccattt cctacaggaa atacaaaatc cgaggtaccg
     4201 atctttttta cccatcctga aatatgcctg ttgtctgcac taccattatg agttgcaact
     4261 gcctcataaa taagatagtt gggggttgct gaagtcgtaa taatcccgtt ggtaaaagta
     4321 tgagtgccgg aaacagtcaa attattatta agtactattc ctgcgatatt atcagttacc
     4381 aggtcagatg ttttaattag ctctgtacct gcaatagttt ggattgatgt gccatttaaa
     4441 tatagtgtac cagtccctgc cgacattaaa gcctgatgat ttgtcaaatt ccctttaagg
     4501 tataatgaac cattgttgat taagtttgca gttgaactat tctccacatt accaacaaca
     4561 gccatattag taccggtatg caattgcaga ttaccggagt ttcgaaaacc ggtttgtcca
     4621 aaacacaaac cggaaacgag taccaatata atttcaatgt aaagacattt tctcatacaa
     4681 aagagtattg ataattaatt attgggttgt aacctgccag tttaagtcaa gtattgtgat
     4741 aatatcaccg ctatttgctg aagaacaata aacttcaaag taatcagtgg aagaaatatc
     4801 agatatatat ataacggttg aaaactggga agctgttccg gcagaacttg tcctcagtgt
     4861 agtttcacca tatcgggttg atgtgacgcc atttttcact aatgcaaacg aaatagtcct
     4921 gcttgagtta ttcactgata aattacctgt aatcacaatt acaccatccc tcttattcgt
     4981 aggctgataa gtaattctgt tgttggcgat tgtccatttt gctgtagagg acgctgtatt
     5041 tgtccagttt gccttatacc atgaattggc tgtagtaagt gtagtagtgg tactcccgtt
     5101 aagcacattg atgtagcagc tcggtttttt atctcccata cctgcattac tttccaggat
     5161 aacatcctta tcccggccat cgctgcgggt aaagtcgaag ccttcaatat acttgccaat
     5221 attattccat gaatttcctg taattgagat attggttggt aaggtaaatg tggttggctg
     5281 gtaatatatt gcagtatcac cgcttaatgc attgtaatat cctccggaag ccagttggat
     5341 agttgctgca gatcctttac taaggtttat accataatta cagccaataa aatcagtttc
     5401 agcaacttta acttttacac cggaaacatt actatggacc aaaatgccgc tgccttgtga
     5461 atttgaaata tcggtctcaa aaacccatat ctctgcattg
//
LOCUS       DAORLS010000209         5492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8097_length_5492_cov_2.267611, whole genome shotgun sequence.
ACCESSION   DAORLS010000209 DAORLS010000000
VERSION     DAORLS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_5492_cov_2.267611"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK977_14105"
     CDS             complement(<1..350)
                     /locus_tag="PK977_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF19303.1"
                     /translation="MSLFIASLNSGSNANCYYVGNDQEALLVDAGLSCRETEKRMKQL
                     GLSLQKVKALFVSHEHADHISGVPGLSKKYQLPVYITEGTFRNSSIPVEEPLLRHFRH
                     TKTLEIGSLSVTPFR"
     gene            548..1900
                     /gene="gdhA"
                     /locus_tag="PK977_14110"
     CDS             548..1900
                     /gene="gdhA"
                     /locus_tag="PK977_14110"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRF19304.1"
                     /translation="MIATKNTAVDKFMQLMKKRNPYQPEFLQAVSEVAHAIIPFIEEH
                     PEYKKANILERMTEPERVIIFRVPWVDDEGNFRINRGFRVQMNNAIGPYKGGLRFHPS
                     VNLSILKFLAFEQVFKNSLTTLPMGGAKGGSDFDPKGKSDNEVMRFCQSFMTELYRHI
                     GNDTDVPAGDIGVGGREIGYLFGQYKRLSNQFTGVLTGKGYTYGGSLIRPEATGYGTL
                     FFVEEMLKTKGETLKGKKVVISGSGNVSQYAIEKTIQLGGIPLTASDSNGFIYDPDGI
                     NEEKLAFILELKNVRRGRIAEYADKYAVPYYEGEKPWKIPCDIALPCATQNELDEEDA
                     KMLVKKGCKLVAEGTNMPTTPEAIKVLQDKGVMYAPGKAANAGGVATSGLEMSQNSIR
                     LSWTREEVEKRLQTIMINIHEQCMMYGKKEDGTIDYVKGANIAGFVRVADAMLAEGHV
                     "
     gene            2037..2807
                     /locus_tag="PK977_14115"
     CDS             2037..2807
                     /locus_tag="PK977_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19305.1"
                     /translation="MQRIKTYLLLLLAVLAGNSSILQPYVDPLQVRFTNGFRNNNSTE
                     RGTPFTHLWAGSDIPVKLAPETYLLLSPYYENWQLDSGKKENIVPGVHSLALPIGLLL
                     PLKNKKWSLSFTAILRSNAEKLLADKTIQAGGAGFLSYEKAKSKKLRFGLYVNSDFFG
                     LFVMPLAGADWRINKRNYLFGLLPGRFTWEHQFNNRLFGGLTFRAITNSYRLRNGNYL
                     RIDDNQLSGFLDVYLSRRICFTVEPGYGILRKKRVFAW"
     gene            2916..>3300
                     /locus_tag="PK977_14120"
     CDS             2916..>3300
                     /locus_tag="PK977_14120"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF19306.1"
                     /translation="METTIAIMMADLSGYTALTEIHGGEKAADMVHRFLQLVNQSLVG
                     DSRLHERVGDQVVIVSSSAEQLAYTATFLFERVHEQEWFLALHAGLHYGTVVQKDNAY
                     FGSTINTASRIMSAAEKGKMLCSKEF"
     assembly_gap    3301..3329
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3330..3558
                     /locus_tag="PK977_14125"
     CDS             <3330..3558
                     /locus_tag="PK977_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRF19307.1"
                     /translation="TSSKGLMQFKNLLQPVDLYELHCCIKNISKTYVIDPVCHMLITA
                     PEKAVQLTRQNDTLYFCSEKCRDMYITHHSG"
     gene            complement(3627..>5492)
                     /gene="htpG"
                     /locus_tag="PK977_14130"
     CDS             complement(3627..>5492)
                     /gene="htpG"
                     /locus_tag="PK977_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRF19308.1"
                     /translation="TISINTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKIRRLSS
                     LGHYNKDLGNLNIKVVVDKDAKTITISDSGIGMTADEIKKYINQVAFSGATEFMEKFK
                     EANDANEIIGRFGLGFYSAFMVAEKVEIQTLSYQEGAEPARWICDGSTEFEITEGNRS
                     ERGTDVILYINEDSVEFLEKHRIQSILDKYAKFLPVPVQFGTKTESVEDGTDEEGKTK
                     YKTVEVDNIINNTNPIWTKAPADLKDEDYLHFYQELYPFQDEPLFWIHLNVDYPFNLT
                     GVLYFPKLKNDFEVHKNKIKLFSRQVFITDEVKDIVPEFLLLLHGVIDSPDIPLNVSR
                     SFLQSDSNVKKINSYISRKVAEKLLELFKKDRKAYEEKWSDIGLFVKYGALSDEKFYE
                     KAKEFLLLTNTQKEHYTLEEYSTKVKDFQTDKNGQLVYLYTADAEHQHSYVAAAQKKN
                     YDVLLMNSPIDTHFIHHLEMKLEKTSLKRVDADVLDKLIEKENTEQHKLTQEESDKLK
                     AIFDKAINNVNMKVEVESQPADHLPVTITMEEWMRRMKDMSRLGGGMNFYGAMPDTYK
                     VAVNGNHKLISKILAADEAQQTILAKQAFDLALLAQGMLKGADLTAFVERSVEVL"
BASE COUNT         1523 a         1291 c         1167 g         1482 t
ORIGIN      
        1 cgaaagggag taacagataa gctcccgatc tccaatgttt tcgtatgccg gaaatgccgg
       61 agcaaaggct cctccacagg tatggaactg ttacggaaag tcccttcggt tatataaaca
      121 ggtaactggt atttttttga caaacccggc actccgctga tatggtctgc atgttcatga
      181 gaaacaaaaa gggcctttac tttttgcagg gacaggccaa gttgcttcat tcttttttcg
      241 gtctcacggc aactgagtcc ggcatccacc agcaacgctt cctggtcatt tcccacatag
      301 taacagttgg cattgctgcc ggaatttagc gaggcaataa aaagagacat acttgtccaa
      361 aaatagcaac taatcatcta caggaaagaa atcaatattc ctgtcaatac tcatgtaaaa
      421 ctgaagccgg gactgacttt cctcatttta atcaggcagg gcttgttttt acttttagat
      481 attgtgatga caggatctgg acaatactcc ttgtgaaata agattgctta acatttaaac
      541 catttttatg attgcgacaa aaaatacagc tgttgacaag ttcatgcagc tgatgaaaaa
      601 acgcaaccct taccagcctg aattcctgca ggccgtttcc gaggttgcac atgccattat
      661 tccttttatc gaagaacatc ccgaatacaa aaaggccaat atcctggaaa ggatgaccga
      721 acccgaaagg gtgatcatct tccgggttcc ctgggtagat gatgaaggca atttccgcat
      781 caaccgcggg ttccgggtgc agatgaacaa tgccatcggt ccttataaag gcggtctccg
      841 cttccacccc agcgttaacc tcagtatcct caaattcctc gcctttgaac aggtattcaa
      901 aaactctctc accactttac caatgggtgg ggccaaggga ggttcagatt ttgaccccaa
      961 aggaaaatca gacaatgaag taatgcgctt ctgccagagc tttatgacag aactatatcg
     1021 ccacatcggc aacgacacag acgtaccagc cggtgatata ggtgtaggcg gaagagaaat
     1081 cggttaccta tttggacaat acaaacgcct cagcaatcaa tttaccggtg tgctcaccgg
     1141 aaaaggatat acctatggcg gcagccttat ccgccccgaa gcaaccggtt atggcaccct
     1201 tttctttgta gaagaaatgc tgaaaacaaa aggcgaaacc ctgaagggta aaaaagtagt
     1261 catatccggt agcggaaacg tatcacagta tgccattgaa aaaacaatac agctgggagg
     1321 tataccgctt actgcatccg acagcaatgg cttcatatat gatcccgatg gaataaatga
     1381 agaaaaactg gccttcatcc tcgaactgaa aaatgttcgc cgcggccgca ttgccgaata
     1441 tgccgacaaa tatgctgtgc cttattacga aggcgaaaaa ccctggaaga taccttgtga
     1501 tatcgctctg ccctgtgcca cacaaaatga gctggacgaa gaagatgcaa aaatgctggt
     1561 gaaaaaagga tgcaaactgg tagcagaagg cactaatatg ccaaccactc ctgaagcaat
     1621 taaagtactg caggataaag gagtgatgta tgcaccgggc aaagctgcca atgctggtgg
     1681 cgtagccact tccgggctcg aaatgtcaca aaactccatc cgtctttcct ggacaagaga
     1741 agaagtagaa aaaagattgc agacaatcat gataaatatt catgagcaat gcatgatgta
     1801 tggtaaaaaa gaagacggca ccattgacta tgttaaaggc gcaaacatag ctggcttcgt
     1861 acgggtagca gatgctatgc tggccgaagg ccatgtttag gcaatcaatt cacatgaatc
     1921 aaggcgcaca aggaaacgcc ccggaagaac cggggcgttt ttcatccgtc tcttaacaaa
     1981 tgtcaatttt ttccgggcac actcttgtta cttttattac cataaaagca ccgggcatgc
     2041 aaaggataaa aacatacctc ttgctcctgc tggctgtgct ggcaggaaac tcctccatcc
     2101 ttcaaccata tgtagacccg ctgcaggtaa gatttacaaa tggtttccgg aacaataaca
     2161 gtacggaaag aggtacgccg ttcactcact tatgggccgg tagcgatata cctgttaaac
     2221 tggcgccgga aacctatttg ctgctcagcc cttattatga aaactggcaa ttggattccg
     2281 gtaaaaaaga aaatattgta ccgggagtgc atagtcttgc attgcccata ggcctcctgc
     2341 ttccgttgaa aaataaaaaa tggtcactgt cctttacagc tatactgcgg tcaaatgctg
     2401 aaaaattact ggctgacaaa acgatccagg caggcggggc tggtttcctt agctacgaaa
     2461 aagcaaagag taaaaaatta cgcttcgggt tatatgttaa ctccgatttc ttcgggcttt
     2521 tcgtcatgcc gctggccggg gccgactggc ggatcaataa acgaaattat ctctttggcc
     2581 tcctgcccgg aaggttcaca tgggaacacc aatttaataa ccggctcttt ggcgggctta
     2641 ccttccgtgc catcaccaat agttaccggc ttcggaacgg taactacctg cgcatagatg
     2701 ataaccagct atccggcttt ttggatgttt atctctcccg gcggatttgc ttcacggtgg
     2761 aacccggtta tggtatcctt cgaaaaaaga gagtatttgc ctggtaaaac atgggaggat
     2821 ggattcttta tcaaactcag tacagcatac aggatacgct tcgaaaaata aaatacccac
     2881 ggcttttgtt tgatttcttt cctaattttc acttcatgga aaccacaata gccatcatga
     2941 tggccgacct ttccggctac accgctctca ctgaaattca cggtggcgaa aaagcggccg
     3001 atatggtaca tcgtttcttg caactggtca atcagtcact ggtgggcgat agccgcctgc
     3061 atgaacgggt aggcgaccag gtagtgatcg tttcttcttc ggcagagcaa ctggcctata
     3121 ccgctacatt tttgtttgaa agggtacatg aacaggagtg gtttctcgcc ttgcatgcag
     3181 gactgcatta tggaaccgtg gtacaaaaag ataatgcata cttcggctct accatcaata
     3241 cagcgtcccg catcatgtca gcagcagaga aaggaaagat gctttgctcc aaagagttcg
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna actagcagta aaggattgat gcagttcaaa
     3361 aacctgcttc agccggttga tctatatgaa ctgcattgct gtatcaaaaa tatcagcaag
     3421 acttatgtga tcgatccggt ttgtcacatg cttattacag ctcctgaaaa agcagtgcag
     3481 ctgacacgac agaacgatac cttgtatttc tgctcagaaa aatgcaggga catgtatatt
     3541 acgcatcatt ccggttgata aaatatggct gtctcaaaag gctggcgttt taaaacaatc
     3601 ccaccttaat actaattcaa tcaggattac agcacctcca cgcttcgttc tacaaaggcc
     3661 gtcagatcag ctcccttcag cataccctgc gccagcaggg caaggtcaaa tgcctgtttg
     3721 gccagtatgg tttgctgtgc ctcgtcagca gcaagaattt ttgaaatgag cttgtgattg
     3781 ccattcaccg ctactttata agtgtccggc atggcaccat aaaaattcat accaccaccc
     3841 agccggctca tatccttcat ccggcgcatc cattcttcca tagtaatagt aacgggcaaa
     3901 tgatcggcag gctgactttc cacttccacc ttcatgttta cattattgat cgccttatca
     3961 aaaatagctt taagcttgtc cgactcttcc tgtgtgagtt tatgctgctc agtattttct
     4021 ttttcaatca gcttatccaa tacatccgca tccactctct tcaaggatgt tttctccagc
     4081 ttcatttcca ggtgatgaat gaaatgagta tcaatcggcg aattcatcag cagcacatca
     4141 taattctttt tttgtgccgc cgccacataa ctgtgctggt gctctgcatc ggcagtatag
     4201 aggtatacca gttgtccgtt cttgtccgtc tggaagtctt tcaccttagt actgtattct
     4261 tccagggtat aatgttcctt ttgagtgttg gtaagcaata aaaactcttt cgccttttca
     4321 taaaactttt catcactcaa cgcaccgtat tttacaaaga gccctatatc actccatttt
     4381 tcttcatatg ctttgcggtc ttttttaaaa agctccagga gtttctccgc taccttacgg
     4441 ctgatatagc tgttgatctt tttcacatta ctgtcgctct gcaggaaaga gcggcttaca
     4501 ttcagcggaa tatccggcga gtcgatcact ccgtgcaaca gcagtaaaaa ttccggaaca
     4561 atatccttta cctcatccgt gataaacacc tggcggctga aaagcttgat cttattctta
     4621 tgtacttcaa aatcattctt cagtttcggg aagtataata caccggtaag gttgaaagga
     4681 taatctacat tcaggtgtat ccagaacaat ggctcatcct ggaagggata cagctcctgg
     4741 tagaagtgca ggtaatcctc atccttcagg tcagcagggg ccttggtcca gatggggtta
     4801 gtgttgttga tgatattatc aacttcaaca gttttgtatt tggttttgcc ttcttcatcg
     4861 gttccgtctt ccaccgattc tgttttagtg ccaaactgca ccggcaccgg taaaaatttg
     4921 gcatacttat ccagtatgga ttgaatacgg tgcttctcta aaaactcaac ggagtcttca
     4981 ttgatataaa gtattacatc cgttcctctt tcgctgcggt ttccttctgt gatctcaaat
     5041 tcagtgctgc cgtcacaaat ccagcgggcc ggttcggctc cttcctggta agagagtgtt
     5101 tgtatctcca ctttctccgc caccataaaa gcagaataga aacccaaacc aaagcggccg
     5161 ataatctcgt tggcatcatt ggcttccttg aatttttcca taaactcggt agcgccggaa
     5221 aaagccacct ggttaatgta ttttttgatc tcatcagccg tcataccgat accgctatcg
     5281 gagatggtga tcgttttggc atccttatcc accactactt tgatattcag gttgccaagg
     5341 tccttattgt aatgtcccag cgaactcagt cgcctgatct tctgggtagc gtccactgca
     5401 ttgctcacca gttcccgcaa aaaaatctca tgatcagagt agaggaattt cttgatgatg
     5461 ggaaatatgt tctctgtatt gatcgaaatc gt
//
LOCUS       DAORLS010000210         5478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8131_length_5478_cov_1.626037, whole genome shotgun sequence.
ACCESSION   DAORLS010000210 DAORLS010000000
VERSION     DAORLS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8131_length_5478_cov_1.626037"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>737)
                     /locus_tag="PK977_14135"
     CDS             complement(<1..>737)
                     /locus_tag="PK977_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF19309.1"
                     /translation="SHLYTVNASTGTKKALTSGNYEVQQVSLSKDKKYFYISTNEVHP
                     GEQHFYRLSIADGKKEKLTSMTGSNQVTISPDEKYLAILHSYSNKPWELYLQENFVTG
                     KQAKTNGKIEQITNKAQSDEFKTYAWKDPEVISFPARDGAQVYARLYKPANPQPNKPA
                     VLFVHGAGYLQNAHKWWSSYFREYMFNNMLADNGYYVMDIDYRGSAGYGRDWRTGIYR
                     HMGGKDLSDHVDAVNYLVKNFGVNPKNV"
     assembly_gap    738..863
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<864..>1497)
                     /locus_tag="PK977_14140"
     CDS             complement(<864..>1497)
                     /locus_tag="PK977_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF19310.1"
                     /translation="FGRGSDATTRAGNNPQEQWLKNDQLQYFQVLRERKEKREATEAY
                     NKSLPKEKELRSISIEDKSLQGLTISPDGRFVSYRLFKAAPSTGAKTTIVPSYVTETG
                     FTTDIPARTKVGSQQGSAEYFVYDRVKDTQIAIKTDSIPGIQDLPDYVKDYPKQLEEK
                     KKKPGNRAVNVSSINWSPKGTYAVLDIRSQDNKDRWLMLFDTSSGKIKVLD"
     assembly_gap    1498..1568
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1569..2030)
                     /locus_tag="PK977_14145"
     CDS             complement(<1569..2030)
                     /locus_tag="PK977_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF19311.1"
                     /translation="MRKALVISLSLFISCIVFGQQKLDQLTVEKIMRDPKWIGTSPSN
                     ISWSNDGNTLFFNWNPDRNPADSLYYITLTNKVPAKASIAQKQLLDVENNYNYNQQRT
                     AYVYAKGGDIFLTDVKTGKTRRITHTTDTESNPQFSFNETKIVYNRSQNLYA"
     gene            complement(2111..2464)
                     /locus_tag="PK977_14150"
     CDS             complement(2111..2464)
                     /locus_tag="PK977_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19312.1"
                     /translation="MTKALSLSVVFTLFVFISVSCSKDNDDGGSSLDCSTVAKSFAAD
                     VNPTIQATCNVSGCHATGSTNGPGPLTNYTQVFNARSAIRTAVANGRMPQGSSLSASQ
                     KNSILCWIDSGAPNN"
     gene            complement(2542..>3276)
                     /locus_tag="PK977_14155"
     CDS             complement(2542..>3276)
                     /locus_tag="PK977_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRF19313.1"
                     /translation="ANNYVRNLTVLHTLLGFAISMLLVFRTNTAYDRWWEGRKLWGSL
                     VNNSRNLAMKLNTVIPVDDKWQRSFFRKIIPAYAFALHRHLRKEETRLELFADDEHKH
                     VFQQIDTTKHVPNQTALLMYQHIQTLYRENKISGEQLLYLNAELQSFTEICGACERIK
                     NTPIPFSYSVFIKKFIFFYVMTLPFGFVFQLGYYVIPVVAFICYVLASLELIAEEIED
                     PFGGDENDVPTGLLAQNIQRHIGELI"
     assembly_gap    3277..3342
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3511..>3723)
                     /locus_tag="PK977_14160"
     CDS             complement(3511..>3723)
                     /locus_tag="PK977_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19314.1"
                     /translation="MSKADEATRRGVQHCEDKKMKAEICYNLTYTYFKNKDKVNFASW
                     AIETKKWAEAESVFTKNIGLMEKQLQ"
     assembly_gap    3724..3785
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3786..4357)
                     /locus_tag="PK977_14165"
     CDS             complement(<3786..4357)
                     /locus_tag="PK977_14165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF19315.1"
                     /translation="MKIHLTPLLLLFPFALQAQPGLEFNKRFVESEDRWVAFAMGKDS
                     TQPYGFIYIDAQAGLTLQYEGNFTVGPDGKFISKKATENGSVKIRLEPNSVKVAFLPE
                     NRVKELGHEWVPDWLSIYKQTSDTIGRMVRWGYFYNHWGESAKALTYLEKAYALEPAK
                     EGLEFELAYAYNALDRFNKAIEVLDKAIQH"
     gene            complement(4411..4629)
                     /locus_tag="PK977_14170"
     CDS             complement(4411..4629)
                     /locus_tag="PK977_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF19316.1"
                     /translation="MPIIVNLDVMMAKRKMSLNELSEKVGLTLSNLSILKTGKAKAIR
                     FSTLETICKVLKCQPGDILEYADEKGDE"
     gene            complement(4632..5183)
                     /locus_tag="PK977_14175"
     CDS             complement(4632..5183)
                     /locus_tag="PK977_14175"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRF19317.1"
                     /translation="MEIRIGTKQILVVLNVLSWIIFLGLCVEAGGMIFNTVFALYKPV
                     VAQHFWNEADLSALYAHDKGHFITQTTLMIIPAVMKALLFYLILKLFYNKKLNIARPF
                     NPELTRVVNLIAWLCLGIGLFSFWGINYAAWIKSKGVSMPEIESLRIGGADVWLFMAV
                     VLFVIGQVFKKGTELQTESDLTV"
BASE COUNT         1354 a         1121 c         1091 g         1587 t
ORIGIN      
        1 acattctttg ggttgactcc aaaattcttt accaggtaat ttactgcatc cacatgatcg
       61 ctcaggtctt taccgcccat gtgccgataa atgccagttc gccagtcacg gccataaccg
      121 gcgctgccac ggtaatcaat atccattaca taatagccgt tgtcggctaa catattgttg
      181 aacatatact cccggaaata actgctccac catttgtgtg cattctgtaa atagcctgca
      241 ccatgcacaa acaggacggc tggtttattc ggctgtggat tggccggctt atataatctt
      301 gcatatacct gtgcaccatc cctggcaggg aagctgatca cctccggatc tttccatgca
      361 taagttttga attcatcact ttgtgcctta ttggttatct gctcaatttt tccgtttgtc
      421 ttagcttgtt tgcctgtaac aaagttttcc tgcaaataca attcccaggg tttgttggaa
      481 taggagtgaa ggatggccag gtatttctca tcaggtgaaa ttgttacctg gtttgaaccc
      541 gtcatggaag tcagtttctc cttctttcca tcagcaatac ttaagcgata aaaatgctgc
      601 tcgccgggat gcacctcatt ggtactgata taaaaatatt ttttatcctt tgacaaagaa
      661 acctgctgta cttcatagtt gccgctggtt aaagctttct ttgttccggt agaagcattt
      721 acagtgtaaa gatgtgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnntgtccaa tacctttatt tttccgctgc tggtatcaaa
      901 gagcatcagc cacctgtctt tattatcctg agaacggata tccagtactg catatgttcc
      961 tttgggcgac cagttgattg aactaacatt aactgcacgg ttgcccggtt tctttttctt
     1021 ctcctctaat tgttttggat aatcttttac ataatcaggc aaatcctgta taccaggaat
     1081 tgaatctgtt ttaatggcga tctgtgtgtc ctttaccctg tcgtatacaa aatattcagc
     1141 gctgccctgt tgcgacccca ctttggttct tgccggaata tcagtggtaa agcctgtttc
     1201 ggttacataa ctgggaacga tagtagtctt tgcacctgta gatggcgctg ctttaaataa
     1261 tcgataacta acaaatcttc catcagggct tatggtcaaa ccctgcaggc ttttgtcttc
     1321 aatactgata gaacgcagct ctttttcttt tggcaaactt ttattgtaag cttcagtggc
     1381 ttcccttttt tcttttcttt ctctcaacac ctggaagtat tgcaactggt cattcttcag
     1441 ccattgctcc tgtggattgt tcccagctct agttgtagca tcgctgcctc ttccaaannn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnntg catacaggtt ttggctccgg ttataaacga tctttgtttc attaaaagaa
     1621 aattgagggt tgctttctgt atcggttgta tgcgttatgc gcctggtctt tcctgttttt
     1681 acatcagtta aaaaaatatc tccccctttg gcatacacat aggctgtccg ctgctggttg
     1741 tagttataat tattctccac atccagtaat tgtttctgcg ctatgcttgc tttagcaggc
     1801 actttattgg tcagggtaat ataatacagg gaatcagcag ggttcctgtc cgggttccag
     1861 ttgaagaaga gggtattgcc gtcattgctc cagctgatat tggaagggga ggtgccgatc
     1921 catttcggat cccgcatgat cttttcaacg gtgagctgat ccagtttttg ctggccaaaa
     1981 acaatgcagg aaatgaataa tgataaggag ataaccaggg cctttctcat aattacagtt
     2041 taggctataa atatattgag aaagccgtca tcaaaaacaa actgcgaaca caaatacagg
     2101 aaaggtctga tcagttgtta ggtgcgccac tgtcgatcca gcagagaatg gaattcttct
     2161 gtgaagcaga cagggaactg ccctgcggca tgcggccgtt tgctaccgct gtacgtatag
     2221 cgctgcgggc attgaacacc tgggtataat tggtcagcgg accgggacca ttggtgctgc
     2281 cggtggcatg acaacccgat acattgcagg tagcctgtat cgtagggttt acatcagcgg
     2341 caaatgattt ggccacggta ctgcaatcca gtgaactgcc accatcatca ttgtctttgc
     2401 tgcaagaaac agaaataaaa acaaaaaggg tgaaaactac cgatagggac agagcctttg
     2461 tcatagcgtt ggggtttggg tgaaaattgt ttcaaaataa cgcaaaaaaa tcagctatgg
     2521 ttgccctact tcttataatt gtcagatcag ttctccgata tggcgttgaa tattctgtgc
     2581 cagtagtccg gtaggcacat cattctcatc acccccgaaa ggatcctcta tctcttcagc
     2641 aatcagctca aggctggcca gtacatagca gataaaagct accacgggaa taacataata
     2701 acccagctgg aacacaaaac caaaagggag tgtcatcaca taaaagaaaa tgaatttttt
     2761 gatgaacaca ctgtatgaaa aggggatagg ggtgttcttg atccgttcgc atgctccgca
     2821 gatctctgta aacgattgca actctgcatt caggtacagc aattgttctc cgctgatctt
     2881 attctcccgg tacagggtct gaatatgctg gtacatgagc aaggcggttt gattgggcac
     2941 atgcttcgta gtatctattt gctgaaaaac atgtttatgc tcatcgtctg caaataattc
     3001 taatctggtt tcttctttgc gcaggtggcg gtgcagtgca aaagcatagg ccggtatgat
     3061 cttgcggaag aaagaacgct gccacttatc atccacagga ataacggtat tgagtttcat
     3121 cgccaggttg cggctgttgt ttactaaact gccccacaac ttgcggcctt cccaccagcg
     3181 gtcataagcg gtattggtgc ggaataccag caacatggaa atagcaaaac ccagtagtgt
     3241 atggagtacg gtaagattgc gtacatagtt attagcnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctatcag cagcgggatc
     3361 agtttacgaa aagtttcagc cttgtgaaaa cggaatataa aagtaaacca gtccttgggg
     3421 ttgtacgaga tcatgcgtca aaaataggga gattttgagt cgggggagaa gagtctgtag
     3481 tcatgagtca ttagtatata gcagatttgg ctattgcagt tgcttttcca tgagccctat
     3541 gttttttgta aacactgatt ctgcttctgc ccattttttt gtttcgattg cccaactagc
     3601 aaaattgact ttgtccttat tcttgaaata agtataggta aggttatagc agatctctgc
     3661 tttcattttc ttgtcttcac aatgctgtac acccctgcgg gtagcttcat cagccttact
     3721 catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnttatg ttgtatcgct ttgtccagca cttctatggc cttgttgaag cggtctagtg
     3841 cattataagc ataggccagt tcaaactcca gtccttcttt tgcaggctca agggcatatg
     3901 ctttttcaag gtaggtcagg gctttggccg attctcccca gtggttatag aaatagcccc
     3961 atcgtaccat gcggccgata gtatcactgg tttgtttgta aatggataac cagtccggca
     4021 cccattcatg ccccagttct tttacacggt tttccggcag aaaagccact tttactgaat
     4081 taggttccag cctgatcttt accgatccat tctcagttgc ttttttggaa atgaactttc
     4141 catcaggtcc taccgtaaaa ttgccctcat attgcagtgt tagtcctgcc tgtgcatcaa
     4201 tataaataaa gccataaggc tgtgtactgt ctttacccat ggcaaatgcc acccagcggt
     4261 cttccgattc cacaaagcgt ttgttgaact ctaaaccagg ttgtgcctgt aaagcgaatg
     4321 gaaacaaaag cagtagggga gtaaggtgta ttttcacgat gaggtgtttg actcgatgaa
     4381 attaagaaag atattttgtg gttctttcat ttattcatct cccttttcat ctgcatactc
     4441 caatatatct cccggctggc atttcagcac tttacaaata gtttccaatg tgctaaagcg
     4501 tattgctttg gcctttcctg ttttcaggat ggaaaggttt gacagcgtca gccctacttt
     4561 ttccgacaat tcgttaagcg acattttacg cttcgccatc atcacatcta agtttacaat
     4621 tatcggcata gttatacggt taaatcgctt tctgtttgta attcggttcc ttttttaaat
     4681 acctggccta tcacaaaaag taccacggcc ataaatagcc atacatcagc gccaccaatc
     4741 cgtaatgact ctatttcagg catacttact cccttacttt tgatccatgc cgcatagttg
     4801 ataccccaga aggagaacaa tccaatacca aggcaaagcc atgctataag atttactaca
     4861 cgggtaagtt ccgggttgaa aggtcgggct atgttcaact ttttgttgta aaacaatttt
     4921 aagatcaggt aaaagagcag ggctttcatt acggccggaa tgatcatcag tgtggtttgt
     4981 gtaatgaaat gtcctttatc atgtgcatag agcgccgaga gatcggcctc attccagaaa
     5041 tgctgtgcca caaccggctt gtaaagggca aatacggtgt tgaaaatcat gccccctgcc
     5101 tctacgcata agcccaggaa aatgatccat gacaatacat taagcactac taaaatttgt
     5161 tttgttccaa ttctgatttc cataatgtag ttgtttaatt caggacaaaa gtataaatat
     5221 ttattgaaaa acaataaaaa tagattgaaa tgtgaaaaat attttcttta atggtaattc
     5281 ggcatccgga gaaccctgca actgaaaagc tgttcgggaa agagagtgtt gaacgcaggg
     5341 gctgaaacgg gggatggccc tcaatgggtt gtagccttat ttctcaaccg gattttactc
     5401 caatcctatc gcccggtttt agaatggggt atgctttttt gatttcacca tactttatct
     5461 gtataacctc tttatcaa
//
LOCUS       DAORLS010000211         5441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8206_length_5441_cov_2.270887, whole genome shotgun sequence.
ACCESSION   DAORLS010000211 DAORLS010000000
VERSION     DAORLS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8206_length_5441_cov_2.270887"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>539
                     /locus_tag="PK977_14180"
     CDS             <1..>539
                     /locus_tag="PK977_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRF19318.1"
                     /translation="AFLILMGGGVVANVVLNKTNGQNSGWIVITFGWAMAVFLGVYSS
                     QKLGGGGHLNPAVTLALLAFGTFKSSLVTTYLLAQLSGAFAGAVLVWLAYKKHFDAST
                     DADLKKAIFCTAPAIRSPLHNAITEFIGTFVLMFGALALSPSAHSLGSLDALPVSLLV
                     LGIGLSLGGPTGYAINPAR"
     assembly_gap    540..566
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            693..1208
                     /locus_tag="PK977_14185"
     CDS             693..1208
                     /locus_tag="PK977_14185"
                     /inference="COORDINATES: protein motif:HMM:NF025939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF19319.1"
                     /translation="MKKVTSFFFFALLVSAATAQNPESKLKELNIELPAIGKPIANYV
                     HVVRTGNLLFLAGKGPSDANGKYITGKVGRELTIEEGQQAARLTAINQLAVLKAELGD
                     LNKVKRVVKVLGMVNCEADFKDHPKVINGFSELMVELFGEKGKHARSAVGMCSLPMNM
                     AVEIELVVEIE"
     gene            complement(1220..2398)
                     /locus_tag="PK977_14190"
     CDS             complement(1220..2398)
                     /locus_tag="PK977_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRF19320.1"
                     /translation="MNQRELFLRHVGQTSPAPLALEIVKADCCTLYDAVGMEYIDLIG
                     GISVANVGHRHPKVITAIQQQLEAYLHVMVYGEFIESPQVQYAQLLTAHLPASLNSVY
                     FTNSGAEAVEGAMKLAKRVTNRTRIIAFKNSYHGSTQGALSIIGDEYWRNAFRPLLPD
                     VLHLDYGSFDALKEITEQTACVIAETVQAEAGIKVPSSEWILALRDRCTETGTLLILD
                     EIQAGFGRTGKLWGFEHFNIVPDVLLLGKALGGGMPLGGFIANKKMMDAFTENPVLGH
                     ITTFGGHPVCCAAGMAGMKALLEEGWIEKVKAKEQLFLSLLKHKKIKAVRSFGLWMAV
                     GFDSFETSKKIIDQCIQSGVLTDWFLFAPNCLRISPPLIIAEEQIKMACDIILKACND
                     "
     gene            2505..3023
                     /locus_tag="PK977_14195"
     CDS             2505..3023
                     /locus_tag="PK977_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRF19321.1"
                     /translation="MPVILPVENKHPQFGNNNFIAPNATIVGDVITGNDCSFWFNTVV
                     RGDVNFIRIGNKVNIQDGACIHCTFQKYGTSIGNNVSIGHNAIVHGCTLHDNVLVGMG
                     AIVMDNCVVHSNTIIAAGAVVLENTICEAGSIYAGVPAKKVKDISQELIHGEIDRIAN
                     NYVRYADWFREK"
     gene            3137..3331
                     /locus_tag="PK977_14200"
     CDS             3137..3331
                     /locus_tag="PK977_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19322.1"
                     /translation="MKNLRFFALGLSFMVLLSSCGMLSNLFKRKTGCPTNGKNVGAEK
                     LISGDPKATKDAKKAKKFRS"
     gene            3398..3727
                     /locus_tag="PK977_14205"
     CDS             3398..3727
                     /locus_tag="PK977_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19323.1"
                     /translation="MSLTLRTTLGCTEAVIADDGGLKEFYRAANILSQDFNVSFSHKE
                     DDFDTIDWEFRFKGHNMTLHYSIYNGVSLFPTKTRDALSKDNKAVVELANALEEKFFN
                     EGQQKIA"
     gene            complement(3724..4650)
                     /gene="rsgA"
                     /locus_tag="PK977_14210"
     CDS             complement(3724..4650)
                     /gene="rsgA"
                     /locus_tag="PK977_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRF19324.1"
                     /translation="MKVKVYKSTGSWYTLKDETGKFWNGRIKGVLKLDDITSTNPVAV
                     GDWVEAEPENENDKTAVISEILHRHNYINRQSPRHKHQHHIVAANLDQSMLIATLKEP
                     RTSQGFIDRFLVASEMYHVPALIVFNKSDLYKSKELEKYKAWKEMYEAIGYKVLLVSA
                     EKKEGIENLTILLKDKVTLVSGHSGVGKSSLLNVIFPNMNLKTGDISGWSGKGQHTTT
                     FAEMFDLPGGGQIIDTPGMREFGLVDIEKQEVSHYFPEMRDRLNDCQFNNCLHINEPG
                     CAIKEAVVNGDIHEDRYVSYVSILDSIEEKKY"
     gene            complement(4678..>5441)
                     /locus_tag="PK977_14215"
     CDS             complement(4678..>5441)
                     /locus_tag="PK977_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF19325.1"
                     /translation="NEEQRTEGEGIDIVLCMDVSGSMGSRDILPSRMEVAKEVAEEFV
                     RSRPVDRIGLVIFSGESFTQCPVTTDRNTLISQIQHLESRRYLTDGTVIGEGLATAVD
                     RLSQSSGKSKVIILLTDGKEDPPETRLIDPLTALEIAKSKGVKVYTIGMSAGPSSVPE
                     ITNRPAGRNPAVDFLDEELLRKIANETGGQYFRAKDKDALKETYKQIDQLEKSKVEVT
                     TNRRYEERFLPFVLAALLFLFLGVLLRYAVFRKFP"
BASE COUNT         1489 a         1259 c         1120 g         1546 t
ORIGIN      
        1 gcctttctga tcctgatggg tggaggcgtt gtggccaacg tggtattaaa caaaaccaac
       61 gggcaaaaca gcggttggat cgttatcaca tttggatggg ctatggctgt attcctcgga
      121 gtatatagtt cacaaaaatt gggaggaggc gggcatctga accctgctgt aacccttgca
      181 ttgcttgctt tcggaacatt taagtcatca ttagtaacta cttacctgct ggcacagctg
      241 tccggggcat ttgccggtgc agtactggtt tggcttgctt ataaaaaaca ttttgatgca
      301 tccacagacg cagatttgaa gaaagccatt ttctgcacag cccctgctat acgaagtcct
      361 ttgcataatg caatcacaga gttcatcggc acatttgttt tgatgtttgg cgcattggca
      421 ctttcgccct ctgcccattc tctcggcagc cttgatgcac ttcccgtttc attattggtg
      481 ttgggcattg gcttgtcctt aggcggacca acaggctatg ccattaatcc tgcaagggan
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnattt tttcttaccc atacccggaa aaagagatag
      601 cgactgggca tatagctggg tacccatcat agcacccata gcaggggcta tgagtgctgc
      661 atggatattc tcacagctta attaattgtc agatgaaaaa agttacctcc tttttctttt
      721 tcgcattatt ggtatctgct gccactgcac agaatcccga atcaaaactc aaagaactga
      781 atattgaatt acccgctatc ggcaaaccta ttgccaatta tgtgcatgtg gtacgaaccg
      841 gtaatctttt gtttcttgca ggcaaaggcc cttccgatgc aaacggaaaa tacatcaccg
      901 ggaaagtagg cagggaatta accatagaag aaggccagca ggcagcaagg ttaacagcca
      961 ttaatcagct ggccgtattg aaagctgaac tgggtgacct gaacaaagta aaacgggttg
     1021 taaaagtgct gggcatggta aactgtgaag cagactttaa agaccatccg aaagtgatca
     1081 atggattttc agaactgatg gtagaactgt tcggcgaaaa agggaaacat gcaaggtctg
     1141 ccgtgggcat gtgttctttg ccgatgaata tggcggtgga gattgaattg gtagtagaaa
     1201 ttgagtaatt ccaattaggc tagtcattac aagcttttaa tataatatcg caggccattt
     1261 ttatctgctc ttcagcaatg atcaaaggtg gagaaatacg aaggcagttg ggggcaaaca
     1321 aaaaccagtc tgtcagtaca ccagattgaa tacattgatc aattatcttt ttactggtct
     1381 caaaagagtc aaaccccact gccatccaca aaccaaaaga acggaccgct ttgatctttt
     1441 tatgttttaa taaagacaga aacaattgtt cctttgcttt gactttttca atccaacctt
     1501 cctctaataa agctttcata cccgccattc cggctgcaca gcaaaccggg tgtcccccga
     1561 atgtggtaat atggcccagc accgggttct ccgtaaaggc atccatcatt tttttattgg
     1621 ctataaagcc ccccagcggc ataccaccac ctaaagcctt acccaataaa agcacatccg
     1681 gaacaatatt aaaatgttca aagccccata gctttcccgt gcggccaaag cctgcctgta
     1741 tttcatccag tatcagcaat gtccccgttt ctgtacaccg atctcttaaa gccagtatcc
     1801 attctgatga cggcactttg atccctgcct cagcctgtac tgtttctgcg attacacagg
     1861 ctgtttgttc agttatttct ttcaacgcat caaaagaacc ataatcaaga tgcaatacat
     1921 ccggtaacaa gggccgaaac gcattgcgcc agtattcatc accaataata ctcaaggcgc
     1981 cctgtgtact gccatgataa gagtttttaa acgcaatgat tcgggtgcgg ttggttaccc
     2041 gtttggccag tttcatggcc ccttctactg cttcagctcc ggaattggta aaataaacgg
     2101 agttcaatga tgctggtaaa tgcgctgtca gcaattgggc atattgcacc tgcggacttt
     2161 caataaactc accatacacc attacatgca ggtaggcttc cagttgctgt tgtatggctg
     2221 ttattacttt gggatgcctg tgtcccacat tagccacact gatgccgccg atcaggtcaa
     2281 tatattccat tccgactgca tcatataatg tgcagcagtc ggctttcact atttccagtg
     2341 caagcggagc aggtgaggtc tggcctacat gtcgcaaaaa gagttcccgc tggttcaaat
     2401 aaattatttc acacaaagaa cgcaaagggt cagcaaaggc gcaaagaaat tctcaacttt
     2461 acacttaacc tcaaacttta aacttaaaac caaaaacaga atttatgccc gtaatacttc
     2521 ccgtagaaaa taaacaccct caatttggta acaataattt tattgctcct aatgctacca
     2581 ttgttggtga tgtaattacc ggtaatgact gcagtttctg gtttaataca gtagtaagag
     2641 gggatgttaa ctttatccgg attggcaaca aagtaaatat ccaggacggg gcctgcatcc
     2701 attgtacttt tcagaaatac ggtacctcca tcggcaataa cgtttctata ggtcacaatg
     2761 caatcgtaca tgggtgtacc ctgcatgata atgtgctggt aggcatggga gctattgtta
     2821 tggacaattg tgtagtgcat agcaatacta tcattgcagc aggggcagta gtgctggaaa
     2881 atactatttg tgaagccggc agcatttatg ccggagtgcc tgccaaaaaa gtgaaggata
     2941 tttctcaaga gcttatacat ggagaaattg accggatagc gaataattat gtacggtatg
     3001 cagattggtt cagagagaag tagaatgggg actaaggcca agtctctgac cctctgtccg
     3061 ggcttcaatc tagtaacttt actatagccg ggcaattaat aactcttaaa ttcgttattc
     3121 aattaagctg taaacaatga aaaaccttcg attttttgct cttggcctga gtttcatggt
     3181 tttattatca tcctgcggga tgttgagcaa tctctttaaa agaaaaacag gctgcccgac
     3241 caatggtaag aatgtgggag ccgaaaaatt gattagcgga gaccctaagg ctaccaaaga
     3301 tgccaagaag gcaaaaaaat tcagaagtta agttaacatt aagtgcagct tattgttata
     3361 aaatcctgtc taatgaaaaa actaaaacac cttaactatg agcctcaccc tgcgaaccac
     3421 tctcggctgt accgaagccg taatcgccga tgacggagga ttgaaagaat tctaccgtgc
     3481 cgccaacatc ctcagccagg acttcaatgt cagtttttcc cataaagaag acgattttga
     3541 tacgattgac tgggaatttc gtttcaaagg tcacaacatg accctgcatt acagcattta
     3601 caatggtgtt tcactcttcc ctactaaaac ccgggatgcc ctgagtaaag acaataaagc
     3661 tgtagtggaa ctggccaatg ccctggaaga aaaattcttt aacgaagggc agcagaaaat
     3721 agcctagtat ttcttttctt ctatggaatc aagtatgctg acatagctca catacctgtc
     3781 ttcatgtata tccccgttta ctaccgcctc tttaattgca caacccggtt catttatatg
     3841 caggcaatta ttgaactggc aatcgttcag cctgtctctc atttcgggga agtaatgtga
     3901 tacttcctgt ttctctatat ccaccaggcc aaattcccgc ataccggggg tgtcgataat
     3961 ctgcccgcca cccgggaggt caaacatttc tgcaaaagtg gtggtatgct gtccctttcc
     4021 gctccatccg ctgatgtcgc ctgttttcag gttcatattg gggaatatta cattcagcaa
     4081 tgatgactta cctacaccgc tgtgtccgct taccaaagtg accttgtctt ttagcaatat
     4141 tgtcaaattt tctattccct ctttcttttc agcacttaca agcagcactt tgtaaccaat
     4201 tgcttcatac atttctttcc atgccttata tttctccagc tctttgcttt tataaagatc
     4261 gcttttattg aaaacgatca gggccggcac atggtacatc tctgaagcca ccagaaaacg
     4321 gtcaataaat ccctgtgatg tccggggctc ttttaaagta gcaataagca ttgattgatc
     4381 caggttggct gctacaatat gatgctggtg cttatgccgc ggcgactggc ggttgatata
     4441 attgtgccgg tgaaggattt cgctgataac agccgtttta tcattttcat tttcgggttc
     4501 agcctctacc cagtcaccaa ccgccactgg attggtactg gtaatatcat ccagtttgag
     4561 cacacccttt atccggccat tccagaattt gccagtctca tctttcaagg tgtaccagct
     4621 tccagtggat ttatatactt taaccttcat tgacctgcaa gataactgaa tattgtctca
     4681 gggaaatttt ctgaaaacgg cgtatcgcaa taatacccct aagaaaagaa acaaaagtgc
     4741 cgctaatacg aaaggcaaga accgttcttc ataacgccgg ttggttgtca cttctacctt
     4801 cgatttttcc agctgatcaa tttgtttgta cgtttctttc agtgcatcct tatcttttgc
     4861 cctgaaatac tgaccgcctg tttcattggc gattttgcgg agcagttctt catcaaggaa
     4921 atcaacagcg ggattcctac cggccggacg attggttatt tctggaacgg atgaaggccc
     4981 tgcactcatc ccaatcgtat aaaccttaac cccctttgac ttggcaattt ccaaagccgt
     5041 tagcggatca ataagcctgg tttcaggcgg gtcttctttc ccgtcggtca gcagaatgat
     5101 aaccttgctt tttccactgc tttgggataa acggtcaaca gccgttgcca acccttcgcc
     5161 aattactgtt ccatcagtaa gataacggcg actctcaaga tgttgtattt gtgagatcag
     5221 ggtgttacga tctgttgtaa cagggcattg tgtaaagctt tctcctgaaa aaattactaa
     5281 cccgatcctg tcgaccggac ggctgcggac aaattcctcc gctacttctt ttgccacttc
     5341 catccgggat ggcaaaatat ctcttgaccc catactacca cttacatcca tacaaagtac
     5401 aatatctatt ccctcccctt ctgttcgctg ctcctcattc t
//
LOCUS       DAORLS010000212         5425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8239_length_5425_cov_1.395531, whole genome shotgun sequence.
ACCESSION   DAORLS010000212 DAORLS010000000
VERSION     DAORLS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_5425_cov_1.395531"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK977_14220"
     CDS             complement(<1..285)
                     /locus_tag="PK977_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF19326.1"
                     /translation="MSIRCVIIDDEPLARKGLCEYISDTNFLKLEGEFDSPLKAADVI
                     TSGTAQLLFLDIEMPRISGLDFFKTLIQPPPVIFTTAYPQYALEGFEVNAL"
     assembly_gap    303..369
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<370..1323)
                     /locus_tag="PK977_14225"
     CDS             complement(<370..1323)
                     /locus_tag="PK977_14225"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF19327.1"
                     /translation="MYNNNPFIKYRLHHLFFWVAVFFVWYYFRVDDYAEKATAFRVTL
                     IKVIDLAMMIYITNYVFVPLLLYRKKFLWFGFAFVSLILISSIVKMNILGRMLNNPAL
                     YNWSSNWKGRVYDNVLPHFFLVTAGVAVKLLFDYSRLQRRVMEIAKEKAEAELNFLKS
                     QINPHFIFNSLNSVYFLIEKENKDARTALQKFSDMLRYQLYEMNGARIPIEKEVAFLK
                     DYMDLQRLRIDEKYEVQFNLDKSVNGLQIEPLLLIPLVENAFKYVSHHTGKKNFVHVS
                     IGRENGSFCLEVENSKEDHSSLNTGTGGIGLQNVQRRLELLY"
     gene            complement(1419..>1599)
                     /locus_tag="PK977_14230"
     CDS             complement(1419..>1599)
                     /locus_tag="PK977_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19328.1"
                     /translation="TYSFRTGDQASYISLSKGTIDVSFDKEKKLWFLKVNGMIANLVD
                     RSVTYYKVKADLSVL"
     assembly_gap    1600..1668
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1669..1893)
                     /locus_tag="PK977_14235"
     CDS             complement(<1669..1893)
                     /locus_tag="PK977_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19329.1"
                     /translation="MRIFFTFLGLVFFLSANAQTERDTVLKRCPVFITDTVSSNNFFI
                     EARPATLKVYRVKGKLTIAVEQRDQFFTLFF"
     assembly_gap    1912..2042
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2043..2629)
                     /locus_tag="PK977_14240"
     CDS             complement(<2043..2629)
                     /locus_tag="PK977_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRF19330.1"
                     /translation="MAINKENYNRINGELGACNVALVAVSKTKPVDEIRELYDLGHRD
                     FGENYVQEMAEKAEKLPKDIHWHFIGHLQTNKVKFIIPFVQLIQGVDSLKLLKEINKQ
                     ADKAGRVVECLLQMHIAQEETKFGLDNNELKEVLGSLDELKSVKVVGLMGMASFTEDM
                     DKVRKEFCRLKTIFDEVKQSHPDFHILSMGMSSDY"
     gene            2697..3311
                     /locus_tag="PK977_14245"
     CDS             2697..3311
                     /locus_tag="PK977_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19331.1"
                     /translation="MLLRSYLLLACIFCAAVISLAQKTYPGTLVTKLGQELEGEITVN
                     LEGANGDLIKITTIVKSSNKGKKETITTSASYNTAIIKHIIVDNITFYFRTINTAYEK
                     SLKNVCVRLIYGTVNCGLFQSGDGTGQHSIAVKFPKSSFNELNSSEYYDESTFTVAIQ
                     LSECKSLYKKIVNKDDTVSWTEKSNREQRIQAYKNIITEYNSCQ"
     gene            complement(3314..4471)
                     /locus_tag="PK977_14250"
     CDS             complement(3314..4471)
                     /locus_tag="PK977_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRF19332.1"
                     /translation="MLFQLNEEQLMIQKAARDFAQNECLPGVIERDEKQIFPREQIMK
                     LADLGFLGMMVDPQYGGAGMDTISYVLAMEEISKIDASVSVCMSVNNSLVCYGLQEYG
                     TEEQKQKYLVPLAQGKKDGELYIGAFMLSEPEAGSDATSQRTTAEDKGDHYLLNGTKN
                     WITNGGSASVYLVMAQTDASKGSKGINCLIVEKDWPGVVVAAKENKMGIRGSDTHTVM
                     FNDVKVPKENRVGEDGFGFKFAMKTLAGGRIGIASQALGIASGAYELAREYSKTRKAF
                     GTEIMNHQAIAFKLADMATRIEAARLLCLKAAWEKDNHLDYTLSSSMAKVYASETAMW
                     TTTEAVQVHGGYGFVKEYHVERLMRDAKITQIYEGTSEVQRIVISRSILKG"
     gene            4627..>4891
                     /locus_tag="PK977_14255"
     CDS             4627..>4891
                     /locus_tag="PK977_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19333.1"
                     /translation="MKKIHIALLVMVAGAIALLLTFLKTTSTYDTVDTAMSKPGKYVH
                     LMARLDKTQPIEYDAVKNPNYLSFTAVDTLGKSVKVVYHNAKPD"
     assembly_gap    4892..4998
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5102..>5425
                     /locus_tag="PK977_14260"
     CDS             5102..>5425
                     /locus_tag="PK977_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HRF19334.1"
                     /translation="MDYIGEHLLPGQLGHFFIILSLVASLVATFAYFKSSQASLAENA
                     GYWKKLARIAFITEVISVFAIFGILFYIIYNHLFEYKYAWQHSSRSLELKYLLACFWE
                     GQEGSF"
BASE COUNT         1515 a         1113 c          899 g         1524 t
ORIGIN      
        1 cagggcattt acctcaaatc cttccagagc atattgtgga tatgctgttg taaatattac
       61 tggtggcggt tgtatcaggg ttttaaaaaa gtctaaccct gatatacggg gcatttctat
      121 gtcaagaaat agaagttgag cagtaccgct tgttatcaca tcagccgctt ttagtggcga
      181 gtcaaattct ccttccagct taagaaaatt agtgtcacta atatattcac atagcccttt
      241 cctggctaat ggctcatcat cgattatgac acaacgaata ctcatgatag ctgaattttg
      301 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnng tacaataatt cgagccttct ttgtacgttt tgcaaaccaa ttccaccagt
      421 accggtattc aaggatgaat gatcttcttt tgagttttcc acttcaaggc aaaaactgcc
      481 attttcccgg ccgatactga catgcacaaa atttttcttc ccggtatgat gagaaacata
      541 tttaaaggca ttttctacca gcgggatcaa caacaggggc tcaatctgca aaccgttaac
      601 tgatttatca agattgaatt gcacctcata cttctcatca atcctaagcc gttgcaggtc
      661 catataatct tttagaaaag ctacttcctt ctcaatcggt atcctggctc cattcatttc
      721 atagagttga taacgcagca tgtctgaaaa tttctgtagt gcagttctgg catccttgtt
      781 ctctttttca attaaaaaat aaactgaatt cagcgaattg aaaatgaaat gtggattaat
      841 ctgggatttg agaaaattca gctcggcttc agccttttct ttagcgatct ccatcactct
      901 gcgctgtaac cgggaataat caaaaagtaa tttgacggca accccggctg taacaagaaa
      961 aaaatgtggc aggacattgt cgtatacacg acccttccag ttgctgctcc agttatacaa
     1021 ggcagggtta ttcagcatcc tgccaagtat attcattttc acaatactgc ttattagtat
     1081 cagcgaaaca aaagcaaatc caaaccacag gaattttttt ctgtataaga gtaaaggaac
     1141 aaatacataa tttgtaatat agatcatcat tgccaggtca attactttaa ttaacgtcac
     1201 cctgaaggcg gtagcttttt ccgcatagtc gtcaaccctg aagtaatacc agacaaagaa
     1261 aacagcaacc caaaagaaga gatggtgcaa cctgtatttg ataaatggat tgttgttgta
     1321 cataatgcta aataaatgta caaattccgg cgggtaaaca gctttacacc aaaaggcaat
     1381 tttacctgat aaaagggagg ttttttattg aaaaatgact aaagaacaga caggtctgcc
     1441 ttcactttgt aataggtaac tgaccggtcg acaaggtttg caatcatacc atttactttc
     1501 agaaaccata atttcttttc cttatcaaaa gatacatcaa tggttccttt tgaaagtgaa
     1561 atataagaag cctggtcacc ggttctgaat gaataggtan nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa agaaaagggt
     1681 aaaaaactgg tcccgttgct cgactgctat ggtgagcttt ccttttaccc tgtaaacctt
     1741 taatgtggca ggccttgctt caatgaaaaa gttatttgaa ctgacagtgt ctgtaataaa
     1801 taccgggcat cgcttcagca ctgtatccct ttcagtctgc gcattcgcag aaagaaagaa
     1861 aacaagacca agaaaagtaa aaaatatccg cattaccggt ttttaaaggg gnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnttgtaatc actgctcatc cccatggata aaatatgaaa atcgggatga ctttgtttta
     2101 cctcatcaaa aatggtttta agtctgcaaa attctttcct gaccttatcc atgtcctcag
     2161 taaagcttgc cattcccatc aacccaacga ctttgacact tttaagctca tccaggctac
     2221 ccaaaacttc tttcaattca ttgttatcca aaccaaactt tgtttcttcc tgtgctatat
     2281 gcatttgtaa caggcactca acaacccgtc ctgccttgtc agcctgttta ttaatctcct
     2341 tcagcaattt cagactatcc actccctgaa tgagttgcac aaaggggatg ataaatttta
     2401 ctttattggt ctgtaagtga cctatgaaat gccagtggat atcttttgga agtttttccg
     2461 ccttttctgc catttcctgc acatagtttt ctccaaagtc acgatggcca agatcatata
     2521 gttctctgat ttcatcaact ggtttagttt tggaaactgc tactaatgca acattacaag
     2581 cccccaattc tccattgatc ctgttatagt tttctttatt tattgccatt atttataccc
     2641 cttacagggt atgctgttta atctaaaaaa caatattttt aaatcaaata tactttatgc
     2701 tattaagatc ttatttgttg cttgcctgta ttttttgcgc agctgtcatt tccttggcac
     2761 aaaaaactta cccgggaacg ctggttacaa aattaggcca ggagttagaa ggagaaataa
     2821 ctgtaaatct ggagggagca aatggggacc ttatcaagat aactactatt gtaaaatcaa
     2881 gcaataaggg taaaaaagaa acaataacaa catctgccag ctataatacc gctattatta
     2941 aacatataat agttgacaac attactttct atttcagaac tatcaataca gcatatgaaa
     3001 aatcattgaa aaatgtatgt gtccgtttaa tatatggaac tgtaaactgc ggcttgtttc
     3061 aaagtggcga cggaacagga cagcatagta ttgctgtaaa attcccaaaa tcatcgttca
     3121 atgaacttaa ctcctcagaa tattatgatg aatcaacttt tactgtagct atacaactaa
     3181 gcgaatgcaa aagtctttac aagaaaatag taaataaaga cgatactgta tcgtggacag
     3241 aaaaaagtaa cagagaacag cgaattcagg cttacaagaa cataatcact gaatacaata
     3301 gctgccaata gaatcatcct ttcaaaatac tcctgctgat cacaatccgc tgcacttcac
     3361 tggtgccctc gtatatctgg gtgatcttgg catctctcat cagtctttcc acatgatatt
     3421 ctttcacaaa accatatccg ccgtgcacct gcactgcttc agtggttgtc cacatcgccg
     3481 tctctgaagc ataaactttg gccatagatg agctcaatgt ataatcgagg tgattgtctt
     3541 tttcccatgc tgccttcagg caaagtaacc tggctgcttc aatccttgtc gccatatcag
     3601 ccagtttgaa agcaatagcc tggtggttca ttatctctgt gccgaaggct ttccttgttt
     3661 tggaatactc ccgtgccagc tcataagcac cgctggcaat gcccagcgcc tgcgatgcaa
     3721 tgccaattct tccgcccgcc agtgtcttca tggcgaattt gaagccaaaa ccatcttccc
     3781 ctactctgtt ttccttaggc actttcacgt cgttgaacat cactgtatgg gtatcactgc
     3841 cacggatgcc cattttattt tctttagccg ccactaccac acccggccaa tccttttcca
     3901 caatcaggca attgatacct tttgagcctt ttgatgcatc tgtttgagcc attaccagat
     3961 aaacagaagc agaaccacca ttagtgatcc agttttttgt accattcagc aaataatgat
     4021 cgcctttatc ttccgctgtg gttcgttgcg aagtagcatc acttcctgcc tccggctcgc
     4081 ttagcataaa tgcaccaatg tataattcac cgtctttttt accctgggcc agcggaacaa
     4141 gatatttctg tttttgttct tcagtaccat actcctgcag gccataacat acaagactat
     4201 tatttacact catacagacg cttacgctgg catctatttt acttatctcc tccatggcca
     4261 gcacataact aatagtgtcc ataccggcac ctccgtattg gggatcaacc atcattccca
     4321 ggaaaccaag gtcggcaagt ttcattattt gctcacgggg aaaaatttgt ttctcatctc
     4381 tttcaattac accgggcaga cattcatttt gggcaaaatc ccgggctgct ttttgaatca
     4441 tcagctgctc ttcattcagt tggaataaca tatggcaatc atttaaaatg ttggcgcaaa
     4501 gataagggca aaggcaggtt gaaaatgagg cagttcggaa tgcaactatt tcgctttttc
     4561 attcaacaaa actaaaatcc gggtcagttt ttagactacc tttgccattt aaaattcttt
     4621 cgttccatga agaagataca tatcgcatta ctggtaatgg tggcgggggc tattgcccta
     4681 cttttgacct ttcttaaaac taccagtacc tacgacacag tagatactgc tatgtcaaaa
     4741 ccaggaaaat atgtacacct gatggcccgg ctcgataaaa cccagcctat tgaatacgat
     4801 gcagtaaaaa acccaaatta cttaagtttc acagctgttg atactttggg caaatctgta
     4861 aaagtggttt atcataacgc taaacccgac annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnat acaaggaaga acaggcaaaa ggcggggtac acccgggcaa
     5041 tgttcccacc aacggatcaa tgagcaaata acaggttttt cttagtaaac aaaaatcagc
     5101 aatggattat atcggagagc atctcttacc aggccaacta ggacattttt ttatcatact
     5161 ttctttagta gcctctcttg ttgccacatt tgcctatttc aaatcatccc aggctagtct
     5221 ggcagaaaat gcaggctatt ggaaaaaact ggcccgcatt gcttttatta ctgaagtaat
     5281 ttctgtgttt gccattttcg ggatactctt ttacatcata tataatcacc tgtttgaata
     5341 taaatatgcc tggcagcata gcagccggtc attagaatta aaatacctgc tggcctgctt
     5401 ttgggaaggc caggaaggta gcttt
//
LOCUS       DAORLS010000213         5393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8308_length_5393_cov_2.032034, whole genome shotgun sequence.
ACCESSION   DAORLS010000213 DAORLS010000000
VERSION     DAORLS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8308_length_5393_cov_2.032034"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>216)
                     /locus_tag="PK977_14265"
     CDS             complement(<1..>216)
                     /locus_tag="PK977_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19335.1"
                     /translation="EKKTDRITNQSMSVDGVGSCMEVMENDADTSEVPTILGNQLQGN
                     PYSVSVMQQASINLYGNSSGIAVNKLYV"
     assembly_gap    217..343
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            698..>1192
                     /locus_tag="PK977_14270"
     CDS             698..>1192
                     /locus_tag="PK977_14270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRF19336.1"
                     /translation="MKRLFVLFAVFLGSGLLATGLNSPMRVKTTKRFDSFSKGPIGSV
                     SIVIDKSDYELNVYDDKGWYATYPVVFGNNSLADKKVQGDKNTPEGKFKIVAKRVHEK
                     WCRFLSLDYPTKDDIEKFNKRKEKGEIPANASIGGGIGIHGTWPHEDFVIDRYKNWTL
                     GCISM"
     assembly_gap    1193..1246
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1266..>1614)
                     /locus_tag="PK977_14275"
     CDS             complement(1266..>1614)
                     /locus_tag="PK977_14275"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF19337.1"
                     /translation="QEHIEASARTIGFGGFPEKPFGLLVTFWIGIERGFNGFKFQTAK
                     FAKDIYCPVLLQWGRNDNLVLPKETNRIFDAIPSSKKKLVVYENSDHESFLFREPQKW
                     RSETEQFLSSNTR"
     assembly_gap    1615..1663
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1664..2263)
                     /locus_tag="PK977_14280"
     CDS             complement(<1664..2263)
                     /locus_tag="PK977_14280"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF19338.1"
                     /translation="MTTKPAAKKFRYVLKWIGWAILVQFLLLNISAAFHAHKFSHFYD
                     DPSLVNKKPSQNIFRKTWRLFAGQKYGKLPIHQVPVFKCDTIILKTKNGLAIESWFIP
                     ADSAAKGTIILFHGLTMNKEILLPEANDFHYQGYHVMLVDFRAHGNSEGNNTTVGLRE
                     AEEVKLAYDYVAAKGEKNIFCWGTSMGAVAIIKAVSDYQL"
     gene            complement(2340..3428)
                     /locus_tag="PK977_14285"
     CDS             complement(2340..3428)
                     /locus_tag="PK977_14285"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF19339.1"
                     /translation="MIRSLLIILFISFEIISSAQLPAYQQLGLTPPSVNHCTSVKDQF
                     LSGTCWSFSSLSLLESELLKTGKGKVDLSEMFVARHSMVRKIQRHLKLKGSNFFTPGG
                     QFHDVMWVMKNFGMMPEEAYNGKPQGAFNHDHGELDTVLSRFVKLCVRNGITELNQRQ
                     QAFVDSVLDVYLGKIPAEFTFKGRVYTAFSFRDQYMDINPDDYLEITSYTHHPFYTKF
                     VLEDKYNWTADQYWNVPLEDFSRITDEALQNGYTVGWDGDANDPDFDFSSGLAYFKSS
                     NQSTQMQRQVAFTSEATLLDHMMHIVGSTTDKYGHKWYYIKNSWGNYSNPLGGFMFMR
                     EDYFRIRTVAIMVNKQAVPADISEKMRL"
     gene            complement(3491..>4091)
                     /locus_tag="PK977_14290"
     CDS             complement(3491..>4091)
                     /locus_tag="PK977_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF19340.1"
                     /translation="IDEKGAELQGNNVSGNLCIRHPWPSIIRTTYGDHERCRLNYFSA
                     YPNMYFTGDGALRDENGNYRITGRVDDVLNVSGHRLGTAEVENAINMHAGVVESAVVG
                     YPHEIKGQGVYAYVIYEHVHGDENLTRQDILQTVTRVIGPIAKPDKIQFVSGLPKTRS
                     GKIMRRILRKIAEGEMDNLGDTTTLLDPGVVEEIKRGRL"
     assembly_gap    4092..4101
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4102..5391)
                     /locus_tag="PK977_14295"
     CDS             complement(<4102..5391)
                     /locus_tag="PK977_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF19341.1"
                     /translation="MEDPEGFWAEVASHFQWRKKWDKVLDWNFAEPNIEWFKGGKLNI
                     TDNCIDRHLATMGDKPAIIWEPNNPEERTRVVTYNRLHKRVCQFAQVLKNNGVKKGDR
                     VCIYMGMVPELAYAVLGCARIGAIHSVIFGGFSAQSIADRLYDANAEYIVTCDGAYRG
                     NKDIPLKSVIDDALIGNRTIKKVIVYTRTRTPVSMIKGRDVWWEDEMEHAEHQVEQNG
                     AVSFPAEEMDAEDPLFILYTSGSTGKPKGVVHSCGGYMVYTGYSFVNVFQYQPGDIHF
                     CTADIGWITGHSYILYGPLSVGATTMMFEGVPTWPDAGRFWDIIDKYKVNILYTAPTA
                     IRSLMGFGLGPVHGHKLDSLKVLGTVGEPINQEAWHWYDDNIGKNKCPIVDTWWQTET
                     GGIMISNMAGITPGKPTYATLPLPGIDPVLVDESGNII"
BASE COUNT         1458 a         1241 c          968 g         1486 t
ORIGIN      
        1 aacataaagc ttattaacag caataccact actatttccg tagaggttaa ttgaggcctg
       61 ctgcattact gaaacagaat atggattccc ttgtaactgg ttcccaagaa tagtaggaac
      121 ttcagatgta tcagcatcat tttccataac ttccatgcaa ctgccaacac catcaacaga
      181 cattgattga tttgttattc tgtcagtttt tttctcnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaatgaag ttatagccga
      361 aacaagcaca agtcaatacc ctttttgtag tattttttta cagtaatttt actgccaccc
      421 ccatttctaa acgtcccgtt aaaatccttt ctttagcggg ataagcttat ccacataaaa
      481 aaataaaaga tcatggcatt acgtttgcca tctggacggt tactgatttt agccataaac
      541 agacttcgct cccacctggg cagttgacct ggccttaccg aaaacatata ttaaagatga
      601 aatgagcgat aaaaattttc gagcaactta ctcaatctgc gatgaaaatt tttattgcga
      661 ataacatgtg tcattaccct aaaataaatg ttaccacatg aaacgacttt tcgtactctt
      721 tgctgttttc ctgggaagcg gattgctggc cactggcctt aacagcccga tgcgggtaaa
      781 aacaaccaaa cgttttgaca gttttagtaa aggacccata ggttctgttt ctatcgtcat
      841 cgacaaatcg gactatgaac tgaatgtata tgacgataaa ggctggtatg ccacctaccc
      901 cgttgtattt ggaaacaata gtcttgctga taaaaaagtg cagggcgata aaaacacccc
      961 ggaaggcaag tttaaaatag tggctaaaag agtacatgaa aaatggtgcc gctttctttc
     1021 actggattat cccaccaaag acgatattga aaaattcaat aagcgtaaag agaaaggtga
     1081 aatacctgcg aatgcaagca tcggcggcgg tatcggtata catggcacct ggccacatga
     1141 ggattttgtg attgaccgtt acaagaactg gacccttggt tgcatctcta tgnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacc attaaaaaat
     1261 aatactcagc gggtatttga agaaagaaac tgttctgtct cgcttctcca tttctgcggt
     1321 tccctgaaca ggaaggactc atgatcggag ttttcgtata ccactaattt tttcttggaa
     1381 gacgggatgg catcaaatat tcgattagtt tctttaggta gcaccaggtt atcattccgg
     1441 ccccattgta acaacaccgg gcaataaata tcctttgcaa attttgccgt ctgaaatttg
     1501 aatccgttga agcctctttc aattcctatc cagaaggtaa ccagcagacc aaaaggcttt
     1561 tccgggaaac ctccgaaacc aattgttctt gcacttgctt ctatatgctc ctgcnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaactgg taatcagaaa
     1681 cagctttgat gatggccact gctcccattg atgtgcccca gcaaaaaata ttcttttctc
     1741 cttttgcagc gacataatca taagccagtt tcacttcctc ggcctctctt aatccaaccg
     1801 ttgtattatt tccctcgctg ttaccatgcg ccctgaaatc caccagcatc acatgataac
     1861 cctggtaatg aaaatcattg gcctccggca gcagaatttc tttattcata gtcagaccat
     1921 gaaaaagaat aatagtccct ttagctgcag agtctgccgg aataaaccag gattcaatgg
     1981 caagcccatt ttttgttttc aggattatcg tatcgcattt aaaaaccggc acctggtgaa
     2041 tgggaagctt accatacttc tgcccggcaa acaacctcca tgttttcctg aatatattct
     2101 ggcttggctt tttattaacc agtgaaggat cgtcataaaa atgggaaaac ttatgggcat
     2161 gaaatgcggc gctgatattc agcaggagga actgaaccaa aatggcccat cctatccact
     2221 tcaatacata acggaatttt ttggcagcag gttttgtagt cataagatat tgtgctgtaa
     2281 acagctgacc gccaaaagta atttttaacc ggcaaatgcc ataagattcc ttgtactttt
     2341 tacagcctca ttttttcact tatatcggcg ggaacagcct gcttattaac cataatggca
     2401 actgtccgga tacggaaata atcttcccgc ataaacataa aaccacccaa tgggttggaa
     2461 taattgcccc aggaattttt gatataatac catttatggc catacttatc tgttgtacta
     2521 cctacaatat gcatcatatg gtccagtaaa gtagcttctg atgtaaaagc aacctgcctt
     2581 tgcatttggg tagattgatt ggatgattta aaataggcta agccactgga aaaatcaaaa
     2641 tcagggtcat tggcgtctcc atcccagcca accgtatacc cgttttgcaa agcttcatca
     2701 gtgatacggg aaaaatcttc caatggtaca ttccagtatt gatctgctgt ccagttatac
     2761 ttgtcttcca atacaaattt tgtataaaaa gggtgatggg tgtaagaggt gatctccagg
     2821 taatcgtcgg gattgatgtc catatattga tccctgaaag aaaaagcagt gtacactctt
     2881 cctttaaaag taaattctgc cggaattttt cctaaataaa catctaatac actatccaca
     2941 aatgcttgtt gccgttgatt caactcagta ataccatttc tcacacataa ctttacaaac
     3001 ctgcttaaca ccgtatccag ttcaccgtga tcatgattaa atgcaccctg gggctttccg
     3061 ttgtaagcct cttccggcat cattccaaaa tttttcatca cccacatcac atcatgaaac
     3121 tgtccgccgg gtgtaaagaa attgctgcct tttagtttca ggtgccgttg aatttttcgt
     3181 accatagagt gccgggccac aaacatttcg ctgagatcaa ccttaccctt tcccgttttc
     3241 agcaattcac tttccaaaag agataagctg gaaaaactcc agcaggtacc ggaaagaaac
     3301 tgatctttta cagaagtaca gtgattgaca gaaggcgggg ttaaacctag ttgctgataa
     3361 gctggcaact gtgcgctgct aataatctca aaactgatga ataaaataat cagcagactt
     3421 ctaatcatag gatacaagat aagtgaagca attttctgcc ccgtacctta agaagggtat
     3481 tttttaaacc ctatagcctg ccccttttaa tttcttccac taccccggga tcaagtaacg
     3541 ttgtcgtatc accaaggttg tccatttctc cctccgcaat tttccggaga atacgccgca
     3601 tgatctttcc gcttcttgtt ttgggtaaac cggaaacaaa ttgaatttta tccggcttgg
     3661 caatcgggcc gatcaccctt gtcaccgttt gcaatatatc ctgccgggtc aggttctcat
     3721 cgccatgcac atgttcataa ataacatacg catagactcc ctgtcccttt atttcatggg
     3781 ggtaacctac cactgcactt tccaccacac cggcatgcat gttaatggca ttttctactt
     3841 cagcagttcc taaacgatgg ccgctaacat ttaatacatc atccactctt ccggtaatac
     3901 ggtagttacc attttcatcc cgcagcgcac catcaccggt gaaatacata ttgggataag
     3961 ctgagaaata attcaaacgg cagcgttcat gatcgccata cgtagtgcgg ataatagaag
     4021 gccatggatg gcgtatacaa agattaccgc ttacattatt gccctggagc tcggcgcctt
     4081 tttcgtcgat cnnnnnnnnn naataatgtt tccgctctca tccaccagca ccggatcaat
     4141 gccgggcaga ggcaatgtag cataggttgg tttacccggt gtaatgcccg ccatattgga
     4201 gatcatgatt ccgcccgtct ctgtctgcca ccaggtgtcg actatcgggc atttattctt
     4261 tccaatatta tcatcatacc aatgccatgc ttcctggttg atgggctcgc ctacggtacc
     4321 cagcactttc aagctatcca gtttatgtcc atgcacaggc cccagtccaa atcccattag
     4381 tgaacgaata gcggtaggag ctgtgtagag aatattcacc ttgtatttat caataatatc
     4441 ccaaaacctg ccagcatccg gccaggtggg aacaccttca aacatcatgg tggtggcgcc
     4501 aacacttagt gggccgtata aaatatagct atgaccggtt atccatccaa tatctgctgt
     4561 gcagaagtga atatctcccg gctgatactg aaatacattt acaaatgaat aacccgtata
     4621 caccatatag ccaccgcagc tatgtactac acctttgggc ttaccggttg aaccggatgt
     4681 atataatatg aacaacggat cctctgcatc catttcttct gccgggaaag aaactgcccc
     4741 gttctgttct acctggtgct cggcatgctc catttcatct tcccaccata catcccttcc
     4801 cttgatcatg gagacaggag ttctcgtccg ggtatataca atcactttct ttatggttct
     4861 gttaccgatc agggcatcat caatcacact cttcaacggg atatctttat taccccggta
     4921 agccccatca caggtaacaa tatattctgc attcgcatca taaagcctgt cggcaatgct
     4981 ttgtgcagaa aagccgccaa atatgacgct gtgtatagcg ccgattcttg cacatcccaa
     5041 aacagcataa gccagttccg gcaccatgcc catgtaaata caaaccctgt cacccttttt
     5101 cacaccatta ttcttcagca cctgtgcaaa ctggcaaacc cttttgtgca aacggttgta
     5161 agtaaccacc cttgtccttt cttccgggtt attgggttcc caaataatgg cgggtttatc
     5221 acccatcgta gcgaggtggc ggtcaatgca attgtccgtg atgttcagct tgcctccttt
     5281 aaaccattct atgttaggtt ctgcaaagtt ccagtcaagt actttgtccc atttttttcg
     5341 ccactggaaa tgagaagcca cctctgccca aaatccttcc gggtcttcga tac
//
LOCUS       DAORLS010000214         5392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8313_length_5392_cov_1.761476, whole genome shotgun sequence.
ACCESSION   DAORLS010000214 DAORLS010000000
VERSION     DAORLS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8313_length_5392_cov_1.761476"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>552)
                     /locus_tag="PK977_14300"
     CDS             complement(<1..>552)
                     /locus_tag="PK977_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRF19342.1"
                     /translation="EGEKEADALLSAMDALLAKDDFAGLKKLIEENKIKCAISDTCNW
                     TDIRQFNLMFSTEFGSTVSSDAEDNKVYLRPETAQGIFVNFLNVQKTGRMKVPFGIAQ
                     IGKAFRNEIVARQFIFRMREFEQMEMQFFIRPGSQKEWYEYWKAERLKWHQALGIPAE
                     KYRFHDHVKLAHYADAACDIEFEF"
     assembly_gap    553..641
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<642..915)
                     /locus_tag="PK977_14305"
     CDS             complement(<642..915)
                     /locus_tag="PK977_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRF19343.1"
                     /translation="MANETNKFQAIISHCKEYGFIFPSSEIYDGLQAVYDYGQMGAEL
                     KKNIKDYWWKSMTQLQDNIVGIDAAIFMHPTTWKASGHVDNFNDPMI"
     gene            1035..1325
                     /locus_tag="PK977_14310"
     CDS             1035..1325
                     /locus_tag="PK977_14310"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRF19344.1"
                     /translation="MKRPVFYMALPVTLIVMLFNQSSSKNCQNKSYYQQIQAGFNAGV
                     DTLRPDPICNMKVDDKKGDTIHYKGHIFGFCSKYCRTEFMKNPDSYLPKKKD"
     assembly_gap    1445..1458
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1459..1711)
                     /locus_tag="PK977_14315"
     CDS             complement(<1459..1711)
                     /locus_tag="PK977_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRF19345.1"
                     /translation="MTILQAQQQVDGWIKTVGVRYFSELTNMAILAEEVGELARIMAR
                     TYGDQSFKKSDMDKNLADEMADVLWVLICLANQTGVDLTD"
     gene            1813..2136
                     /locus_tag="PK977_14320"
     CDS             1813..2136
                     /locus_tag="PK977_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19346.1"
                     /translation="MYAIMKSVSRLVTVILFTVSFFSLQAQSSKKETKPYKVITSGRQ
                     LTIKSSKTISNVMVWTTDGNRVVEQKKINNTSFTIDIPVYRKAFYLMIGLTNGKIYTE
                     KIGVL"
     gene            complement(2210..>2471)
                     /gene="dtd"
                     /locus_tag="PK977_14325"
     CDS             complement(2210..>2471)
                     /gene="dtd"
                     /locus_tag="PK977_14325"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRF19347.1"
                     /translation="SVKDKNGDIILVSQFTLHASTKKGNRPSYIKASKPDMAVPLYEK
                     MIEQLSADLGKNIGTGEFGADMKVELVNDGPVTIVIDTKNKE"
     assembly_gap    2472..2530
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2531..2666)
                     /locus_tag="PK977_14330"
     CDS             complement(<2531..2666)
                     /locus_tag="PK977_14330"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRF19348.1"
                     /translation="MRAVIQRVSKASVTIDGKIHSQIGWGLLVLLGIEDADTAEDIGW
                     L"
     gene            2765..3169
                     /gene="arfB"
                     /locus_tag="PK977_14335"
     CDS             2765..3169
                     /gene="arfB"
                     /locus_tag="PK977_14335"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRF19349.1"
                     /translation="MINVSKEISFQTTRSGGKGGQNVNKVETAVIASLHIESSQLLSD
                     VQKTLIKEKLSNRINTEGYLQVKSQTHRTQLANKEEAVEKINELITKALTPKKPRIAT
                     KASKVSKEKRLEWKKRNAEIKSGRQKFKPGDY"
     gene            3209..4255
                     /locus_tag="PK977_14340"
     CDS             3209..4255
                     /locus_tag="PK977_14340"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF19350.1"
                     /translation="MRKTTLTILSLVTFTITFSWLIGACRKEQKTNGLQLMQQEIPEG
                     FPQPNYRFNDNPLSKEGFELGRKLFYDGRLSIDNLHPCSSCHEQKAGFGTFEHDRSHG
                     VFDSHTLRNAPVLFNLAWSPYLHWDGEFTRLTDAAAQPINGHIEMGESYAGIINKIKD
                     DPYYKEQFKTVFGSPFIRPEFILKALAQFTGYLMSANSKYDRYKKGTATFTAQEESGY
                     QLYKAKCATCHPEPLFTDYSFRNIGLPVDGFLKDYGRMRITGKKEDSLKFKVPTLRNT
                     YISSNYMHDGRFGTLLQCINHYRTGIQQGVTLDPLLVNGINLTNAEVNDLFVFLRALT
                     DSSFLQDPRFARPD"
     gene            complement(4252..4884)
                     /locus_tag="PK977_14345"
     CDS             complement(4252..4884)
                     /locus_tag="PK977_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19351.1"
                     /translation="MKKKVLLSTVMVFLVLISLAQSYEGTIQYDKKKQAAIVCDYSYP
                     AQAVENAFVKRMEKLGYKAKGILNRDKGFLVFKNAYVTDISKDRLDYIIKVERKSRKE
                     SDESIIYMVMLKNDANAISTMEAYDIGKAKSYLNEMLPDIEAANLELQIAAQEEVVTK
                     AEKKLKELQDDKLNLEKKLAENATAQDEAIKDIDAQKKSLEALKEKRKKN"
     gene            4997..>5392
                     /locus_tag="PK977_14350"
     CDS             4997..>5392
                     /locus_tag="PK977_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRF19352.1"
                     /translation="MFTNLSNPLSSIKKLAGQTLWYGASSIFGRFLNYLLTPLLVTIF
                     ASGEYGKISTLFTIAAFLNIVYTYGLETSYFRFAALQAEPKVYNTCSSSIILTTTLFT
                     GLLLLFVKDIAAFLEIPDHPEYISWVILIV"
BASE COUNT         1519 a         1178 c          968 g         1565 t
ORIGIN      
        1 aaattcaaat tcaatatcgc aggcggcatc ggcatagtgt gccagtttca catggtcgtg
       61 gaaacggtat ttctcagcag gtatacccaa ggcctggtgc catttcaacc tttcagcttt
      121 ccagtactca taccattctt tttgtgaacc ggggcggatg aagaattgca tttccatttg
      181 ctcaaactcc cgcatacgga aaataaactg tcttgctacg atctcattac gaaatgcctt
      241 accaatctgt gcaataccaa aaggtacttt catacggccg gtcttctgca cattcagaaa
      301 gttgacgaaa ataccttgtg cagtttcggg acggagatac accttattat cttctgcatc
      361 acttgacaca gtagaaccga attcagtaga aaacataagg ttaaactggc ggatatccgt
      421 ccagttgcag gtgtcactga tggcgcattt tattttattt tcttcaatta gttttttgag
      481 cccggcaaaa tcatcttttg ccagcaatgc atccattgct gataaaaggg catctgcctc
      541 cttctctcct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaatcatag ggtcgttgaa
      661 attgtcaaca tggcccgatg ctttccaggt ggtggggtgc atgaaaatag ccgcatcaat
      721 acctacaata ttatcctgca attgggtcat gctcttccac cagtaatctt tgatgttttt
      781 tttcagttcg gcgcccatct ggccatagtc gtatacagcc tgcaagccat cataaatctc
      841 actgctggga aaaataaagc cgtactcttt acaatgcgaa ataatcgcct ggaatttgtt
      901 cgtttcgttt gccataggcg gcaaagataa tagaacccgg tattatgaaa ctgagcagaa
      961 tcagctcaaa aagacaaacc ctaaatgagg gatgaaatga gtttacaata cctttgaaaa
     1021 gaaatatttt ttatatgaag cgtcccgttt tctacatggc cctgccggtc accttgattg
     1081 ttatgctgtt caatcagtca tcatcaaaaa actgtcagaa taaatcatat taccagcaga
     1141 tccaggcagg cttcaatgcc ggggttgaca cccttcgccc tgatcctatc tgtaatatga
     1201 aagtggatga taaaaaaggt gataccattc actataaagg acacattttt ggcttttgct
     1261 ccaaatactg ccggactgag ttcatgaaga atccggatag ttatctgccg aagaagaagg
     1321 attgataaac tgttaagaat tttcggaaga ttcgttttat cactccccct caggggtcgg
     1381 gggttttaat ttctcattat tccgatgtct ttctgaatca cgaatattct ttttttcaaa
     1441 attcnnnnnn nnnnnnnnca tccgtcagat ccacaccggt ttgattggca aggcagatca
     1501 atacccataa tacatcagcc atttcatctg ctaaattttt atccatatcg gattttttaa
     1561 acgactggtc gccataagtg cgggccataa tgcgggccag ctctcctacc tcttccgcca
     1621 gtatagccat attggtcagt tcactgaaat accggacacc aaccgtctta atccagccgt
     1681 caacctgttg ctgggcctga agtatagtca taacaaaact tttaattgat cactgcaatt
     1741 tagcatgatt cttgttgttt cccatctccc gtccccaatg aaaacccaaa ccaattcctt
     1801 taaaaaatta aaatgtatgc tataatgaag tctgtatcca ggctggtaac cgttatttta
     1861 tttacggtat cattcttttc ccttcaggca caatcttcca aaaaagagac aaagccttac
     1921 aaagtaatta catccggccg tcagcttacc atcaaaagca gtaagacgat tagtaatgtt
     1981 atggtatgga caacagatgg aaacagggtg gttgagcaga aaaaaattaa caatacctca
     2041 tttactattg atataccggt ttaccgaaag gccttttact taatgatagg tttgacaaac
     2101 ggtaaaatat atacagaaaa aattggcgta ctatagccaa ttttgaggcg ttctcccttt
     2161 acctaaagac acttctatct tacctttttg aactcattaa tctcttactc tattctttat
     2221 tcttagtatc tataactatc gttaccggtc cgtcatttac taattccact ttcatatcag
     2281 cgccaaattc accggtacca atattttttc caaggtctgc tgacaattgc tcaatcattt
     2341 tctcatataa aggaacagcc atatccggct tgcttgcttt gatataagaa ggccggtttc
     2401 cttttttggt agaagcatgc agggtaaact ggctgaccaa aatgatatct ccgtttttat
     2461 cttttaccga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn ttagccatcc aatatcttca gcagtgtcag catcttcgat tcccaacagc
     2581 acaagcaacc cccatccaat ctgtgaatgg attttcccat caatggttac actggctttc
     2641 gatactcttt gaatgacggc tcgcatattg gatgctggtt gtgggttatt tgttactggt
     2701 aaagatagct ccgatgaagg aatgattcta tctttatagc ggaaccagta acgattaatc
     2761 agccatgatc aatgtaagca aagaaatatc gtttcaaaca acacgcagcg gaggcaaggg
     2821 cggacagaat gtaaataaag tggagacagc cgtcattgca tccttgcata ttgaatcatc
     2881 gcaactgctg tctgatgtac aaaaaacttt aataaaagaa aaactcagca acaggatcaa
     2941 tactgaaggc tatttacagg taaaatccca aacacaccgt acccagctgg ccaataaaga
     3001 agaggctgtt gaaaaaataa acgaattaat caccaaagcc cttacaccca aaaaaccacg
     3061 cattgccaca aaagccagta aagtgtctaa agaaaaaaga ctggaatgga aaaagagaaa
     3121 cgccgagatc aaatcgggga ggcaaaaatt caaaccgggt gattattaga cctataactt
     3181 gctgtaactt agttattcaa tttaccctgt gcggaagaca actcttacta tactctctct
     3241 tgtcactttt accattactt tttcatggct gattggcgcc tgccgtaaag aacaaaaaac
     3301 caacggcctt caattaatgc agcaggagat acccgaagga tttccgcagc ccaactaccg
     3361 tttcaatgat aatcctttga gcaaagaagg ttttgagctt ggaagaaaac ttttttacga
     3421 tggtcgtttg tcgatagaca acctgcatcc ctgctcttcc tgccatgaac agaaagcagg
     3481 cttcggcact tttgaacatg accggagtca cggggtgttt gactctcaca ccttacgcaa
     3541 tgcaccggtt ttattcaatc ttgcctggag cccctacctg cactgggatg gcgaattcac
     3601 ccgcctgacc gatgcggcgg cccaaccgat taatggacat attgaaatgg gtgaaagcta
     3661 tgcaggtata atcaataaaa taaaagatga cccttactac aaagaacaat ttaaaacagt
     3721 ttttggctca ccttttatcc gcccggaatt tatccttaaa gcattggcac aattcaccgg
     3781 ctacttgatg tcggcaaatt caaaatatga ccgctataaa aagggaacgg ctaccttcac
     3841 ggcacaggaa gaatccggct accagttgta taaagccaaa tgtgccacct gccatccaga
     3901 acctttgttt actgattata gttttcgcaa tatcggtttg ccggtagacg gctttctgaa
     3961 agattacggc cggatgagaa ttacaggcaa aaaggaagat tcattgaaat tcaaagtgcc
     4021 taccctgcgc aatacttata tttcttccaa ttatatgcat gatggccgat ttggcacact
     4081 gttgcaatgc atcaaccact atcgtactgg aatacagcaa ggtgttacac tggatcctct
     4141 gctggtaaac ggaattaacc tgacaaatgc agaagtaaac gatctgtttg ttttcctaag
     4201 ggcattaacg gactcatctt tcctacaaga tccccgtttt gcccggccag actagttttt
     4261 ctttcttttc tctttcagtg cttcaaggct ttttttctgg gcatcaatat ccttgatagc
     4321 ttcatcttgt gcagtagcat tttcagccag ctttttctca aggttcagtt tatcatcctg
     4381 aagctccttc agcttttttt cagctttagt cactacttct tcctgtgctg caatctgtaa
     4441 ttcaaggttg gctgcttcaa tatccggcag catttcgtta aggtaagatt ttgctttacc
     4501 aatatcataa gcttccatgg tggaaatagc attggcatca tttttcagca taaccatata
     4561 tatgattgat tcatcacttt ctttcctgct tttgcgttct accttaatga tataatccag
     4621 tctgtctttg ctgatatcag tgacatatgc atttttgaat accaggaagc ccttatccct
     4681 gttaagaata ccttttgctt tgtagcccaa tttttccatt cttttcacaa acgcattttc
     4741 cacagcctgg gctggataac tataatcaca aacaattgca gcctgttttt tcttatcgta
     4801 ctgaatagtt ccttcgtaag attgagcaag cgatataagt accagaaaca ccattacggt
     4861 tgacagtaaa actttcttct tcataaaaca gcatttgctg ttaaagatac tacaaggctc
     4921 taaagcgttg caaatgtctt aaataaaggg cccggggcag gagtaaattc gttttttgcc
     4981 gggccaaagg ctacttttgt tcacaaatct ttccaatcct ttgagcagca ttaaaaaact
     5041 ggcaggtcag actctctggt acggagccag cagcatcttt ggcaggtttc taaattacct
     5101 gcttacccca ttgctggtaa ccatttttgc ttcgggggag tatggaaaaa tcagtactct
     5161 atttaccatc gcagcattcc tgaatattgt ctacacttac ggactggaaa catcctattt
     5221 caggtttgcg gcattacagg ctgaacccaa agtatacaac acctgttctt cttcaataat
     5281 attaacaaca acactgttta ccgggctgct gctattattt gtaaaagata ttgctgcttt
     5341 tcttgagata cccgatcatc ccgaatatat ttcatgggtc atcctgatcg ta
//
LOCUS       DAORLS010000215         5372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8346_length_5372_cov_2.112469, whole genome shotgun sequence.
ACCESSION   DAORLS010000215 DAORLS010000000
VERSION     DAORLS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8346_length_5372_cov_2.112469"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK977_14355"
     CDS             <1..304
                     /locus_tag="PK977_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF19353.1"
                     /translation="RERVMTAREMQGERYKNNPGIYCNAQMSSKMLKEICVISQVGAN
                     LLKAAMEKLNLSARAYDRILKVSRTIADLAGSNDIKPEHLAEAIQYRSLDREGWAG"
     gene            459..2252
                     /locus_tag="PK977_14360"
     CDS             459..2252
                     /locus_tag="PK977_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF19354.1"
                     /translation="MKILFHYLKPYKWLVALVLLLAAINIGFSLVDPIIFGKLVNLAN
                     HFANNKNEYDVDRFFWSFEENPHYGVAALLIASISVAMISRIAKAFQDYFLNLITQKF
                     GATVFTEGLQHAMKLPYQEFEDARSGETLSVLQKVRIDTEKFVASFINILFPVIVGIF
                     FVAVYAFRIDWRLPLIYFGGIFLLTIISNLLSKKVKTIQKKIVGQTTALAGATTESLR
                     NIELVKSLGLTQQEVSRLNKNTFKILGLELTKVKRIRSISFVQGTFVNTLRQIILFML
                     LWLIFRGEMDAGQLVTMQIFSFFVFGPLQEIGNIILTYREAQASLENFANLMKKAPEP
                     KAANPKHLGEIRTLEFDHVAFKHQTANQNAINDISFKANTGETIAFVGPSGSGKTTLM
                     KLLVGLYRPQQGSILYNGMDENNIDFEDLRNQIGFVTQDTQLFSGTIKENLLFVNPEA
                     TDEELQDVLIKAACQNLLARAEKGLETMIGEGGLKLSGGEKQRLSIARALLRKPKLLL
                     FDEATSALDSLTEEEITDTIRDISRNGKQITILIAHRLSTIMHADRIYVLEKGDVVET
                     GSHENLLAEKGLYYAMWRQQIGERKTKIAIA"
     gene            2255..2569
                     /locus_tag="PK977_14365"
     CDS             2255..2569
                     /locus_tag="PK977_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF19355.1"
                     /translation="MGLLSFLGLGSGKIKNALRQGAIIIDVRSGTEYDRGHVPDAFNI
                     PVDRIKASADRLKEAKRPVILCCNSGERSRKAVEALKAKGLKDVYNGGNWESLLKLMQ
                     SL"
     gene            complement(2574..3008)
                     /locus_tag="PK977_14370"
     CDS             complement(2574..3008)
                     /locus_tag="PK977_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19356.1"
                     /translation="MLKKRIHILSGLAIVFSFVACSKSGSGSDNDSNHLFSANDKTPP
                     EVLIFTPSAGQVFSNGSTINMTGRITDDLGLYRGTIKIVNEATGNTIMLQSYEIHGLR
                     LYNFNISHPVSVTTSADYTVTVSFEDHGANAVSKSVLVKVNP"
     gene            complement(3021..3470)
                     /locus_tag="PK977_14375"
     CDS             complement(3021..3470)
                     /locus_tag="PK977_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRF19357.1"
                     /translation="MELSFNTPALLFPAISLLLLAYTNRFLALANLVRKLHDEYEKGK
                     ENHIVIKQIHSLRYRLRLVRYMQTLGVFSFVCSVLTMYSIYSEWVVVSRYVFATALLS
                     LLISLVISLIEITQSTKALELELSDIEEPEKSGFLSGLISGGNEKKD"
     gene            complement(3541..>3761)
                     /locus_tag="PK977_14380"
     CDS             complement(3541..>3761)
                     /locus_tag="PK977_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19358.1"
                     /translation="NAGVGANITGKKNNVLFSLHLGVTNIGDVAYQNHLSRLKYTAEN
                     NVTGRAGVFNMGRNFSVKLNVPLEFKSR"
     assembly_gap    3762..3776
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3777..>4015)
                     /locus_tag="PK977_14385"
     CDS             complement(<3777..>4015)
                     /locus_tag="PK977_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19359.1"
                     /translation="FENSFSFVRGRFDERIDGFKAGSDNLPLIPAPRWNGELRADFRK
                     AGKFLQNLYARFEVDKTFRQNNHFTGFDTETATDG"
     assembly_gap    4016..4078
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4079..>5028)
                     /locus_tag="PK977_14390"
     CDS             complement(<4079..>5028)
                     /locus_tag="PK977_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF19360.1"
                     /translation="NKSWGYSHLIFSHFNQQLGVVEGDRDDATGEFILFGGSPLERIA
                     TREDLDSRKVFAPNQRVQHNKIVSDNNFAIKKSRLKINLAYQNNLRKEFGNPEDPDEE
                     ELFFDLKTLNYTLHWQLPVKEWHTTLGVNGMRQSNQNKGEEVLIPEYNLFDIGGFVYT
                     QRFFKKGTLSGGLRFDSRSIDSKPFMEGAEEKFTAFTRNFSNISGSIGVSYEPVDFVT
                     VKANIARGFRAPTLAELASNGTHEGTNRFEYGELNLKSETSLQLDGGVEVNYEHISLG
                     ISAFFNRMNDFIFYQKLLSTAGGDSIVLVDGEENLAFRFN"
     assembly_gap    5029..5071
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5072..5356)
                     /locus_tag="PK977_14395"
     CDS             complement(<5072..5356)
                     /locus_tag="PK977_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF19361.1"
                     /translation="MSITRAEVVKGPASLMYGSDALAGVIQFISNLPVANGTYKGNVL
                     MNYQSNNGLLAANANLAGNNNGFNWNINGTYKSAGDYRNKYDGKVLNSRFN"
BASE COUNT         1584 a         1123 c         1062 g         1482 t
ORIGIN      
        1 cagggaaaga gttatgactg caagagaaat gcagggtgaa cgatacaaga acaatcccgg
       61 tatttactgc aatgcacaaa tgagcagcaa aatgctgaaa gaaatatgtg tcatcagcca
      121 ggtaggtgcc aacttgttaa aagcggcaat ggaaaaactg aacctgtctg caagggctta
      181 tgaccggata ctaaaagttt caagaaccat tgctgatctt gccggcagca acgatataaa
      241 gccggaacat ttggcagaag ctattcaata cagaagcctt gaccgggaag gatgggcagg
      301 gtaaaagcag agtcagaacc gaggagccga aagtctaaag tcagaagtca tttgtattac
      361 tttatttact cccggcccaa ctgttatatt ggcatttttc ccgaacttgt acgatgtttg
      421 tacattgatt tcctttcgct ttcacctcaa cagacaatat gaaaatttta ttccactacc
      481 tgaagcccta taaatggctg gtggcacttg ttttactgct tgctgccatt aatattggtt
      541 tctctttggt agacccgatc atctttggta aactggtgaa cctggctaat cattttgcca
      601 ataataagaa cgaatacgat gttgacagat tcttctggtc ttttgaagaa aatcctcatt
      661 acggtgtggc agccttactg atcgcttcta tctccgtagc tatgatcagc cgtatcgcca
      721 aagccttcca ggattatttc ctgaacctta ttacccaaaa attcggcgct accgttttca
      781 cagaaggatt gcaacatgcc atgaaactac cttaccagga gtttgaagat gcccgcagcg
      841 gtgaaacatt aagtgtattg cagaaagtaa ggattgatac agaaaaattt gttgcctcat
      901 ttatcaatat tcttttcccg gtaatcgtag gtatattttt cgtggctgtt tatgcttttc
      961 gtatcgactg gcggctgcca ctcatttatt ttggcggtat ttttctgctg acgatcattt
     1021 ccaacttgct gagtaaaaaa gtaaagacca ttcagaagaa aattgtggga caaaccacag
     1081 cattggcggg agctaccacc gaatcactaa gaaatattga actggtaaaa agtcttggac
     1141 tgacacaaca ggaagtttcc cggctgaata aaaacacttt taaaatactg gggcttgaac
     1201 tgaccaaagt aaaacgtatc cgcagtatca gctttgtgca aggaacattt gtaaatacac
     1261 tcaggcagat catcctgttt atgctgctct ggctcatttt tcgtggagaa atggatgcag
     1321 ggcaactggt gaccatgcag atcttttctt tttttgtgtt tggcccgctg caggaaatag
     1381 gcaatatcat tcttacttat cgtgaagccc aggcatcact ggaaaatttt gccaacctga
     1441 tgaagaaagc gccggaacca aaagcagcca atccaaaaca tcttggcgaa atacgtacac
     1501 ttgaattcga tcatgtggct ttcaaacatc aaacggcaaa tcaaaatgcc atcaatgata
     1561 tcagttttaa agctaatacc ggcgagacga tagcatttgt cgggccatcg ggttccggta
     1621 aaacaacatt gatgaaactg cttgtgggat tataccgtcc ccagcaagga agtatattat
     1681 acaatggtat ggatgaaaat aatattgact ttgaagacct gcgcaaccag ataggatttg
     1741 ttacgcagga cacacagtta ttttctggta ctattaagga aaatcttttg tttgttaatc
     1801 ctgaagcaac tgacgaagaa ttgcaggatg tgttaatcaa agctgcctgc cagaatttat
     1861 tggcaagagc cgaaaaagga ttagaaacca tgattggaga aggcggcctg aaactttcag
     1921 gcggggaaaa gcaacgtttg tctattgcaa gagcattact tcgtaaaccc aagttacttt
     1981 tgttcgatga agctacttca gcacttgatt cgttaacgga agaagaaata actgatacca
     2041 taagggatat ttcgagaaac ggcaaacaga tcactatctt aattgcacac aggcttagta
     2101 ccattatgca tgccgacagg atatatgtat tagaaaaagg tgatgtggtg gaaacaggta
     2161 gtcatgaaaa cctgttagca gaaaagggac tatattatgc catgtggcgt cagcaaatcg
     2221 gggaacggaa aactaaaatc gctatcgctt aattatggga cttttatctt tcttaggatt
     2281 aggatccggt aaaataaaaa atgctttacg ccaaggcgca ataattatag atgtacgatc
     2341 cggaactgaa tacgaccggg gacatgttcc tgatgcattt aatataccag tagatagaat
     2401 aaaagccagt gctgaccgtc ttaaagaagc aaaaagacct gtcatccttt gctgcaactc
     2461 aggtgagaga agccgtaaag ccgtagaggc actgaaagca aaaggtttga aagatgttta
     2521 taacggcggt aattgggaaa gcttgctaaa actaatgcag tccctgtaac tcatcaggga
     2581 ttcactttta ctaaaacaga tttgctgaca gcattggccc catgatcctc aaagctgact
     2641 gttactgtgt aatcggcaga cgtagtaaca gaaaccgggt gactaatatt gaaattataa
     2701 aggcgaagac catgtatttc gtaagactgt aacattatgg tattaccggt agcttcattt
     2761 acaattttga tagtaccgcg gtagaggcca agatcatcag ttattcttcc ggtcatattg
     2821 atagtacttc cattgctgaa aacctgacct gctgatggag taaaaatcaa aacctccggc
     2881 ggcgttttat cattagccga aaacaggtga ttgctatcat tatcactgcc tgatccgctt
     2941 ttagagcagg caacaaatga aaatacaatt gcaaggcctg atagtatatg tatcctcttt
     3001 ttcagcatag tataaatgta ttaatctttt ttctcattgc caccagaaat taagccggag
     3061 agaaaccctg atttttcagg ctcttctata tcactgagct ctagctcaag ggccttggta
     3121 ctctgggtaa tctcaataag ggatatgacc agtgagatca acaagcttag taaggctgtg
     3181 gcgaaaacgt accgggaaac tacgacccat tcactgtaga tgctgtacat ggtgagaacc
     3241 gagcaaacaa aactaaatac acccagcgtc tgcatatacc gaacgagacg aaggcgataa
     3301 cgaaggctat gtatctgctt aatgactata tgattctctt ttcccttttc atactcgtca
     3361 tgaagcttcc ttaccaggtt agccagtgcc agaaacctgt ttgtgtaagc cagcaacaac
     3421 aggctgatgg ccggaaacaa taatgccggt gtattaaatg ataattccat gctgcaagat
     3481 aatttttaaa aagaaacccc gccacaaata tgacggggct taaaatacca gtgataccgg
     3541 ttatctgctt ttaaactcca aaggcacatt caacttgaca gagaaattgc ggcccatatt
     3601 aaacacacct gcccggccgg taacattatt ttccgccgta tattttaacc tgctaaggtg
     3661 attttgatag gccacgtcgc caatattagt aacacccaaa tgcaagctga ataatacatt
     3721 gttcttttta ccggtaatat tagcacccac tcctgcattc annnnnnnnn nnnnnnaccc
     3781 atccgtagcc gtttccgtgt caaagcctgt aaaatgatta ttttgcctga acgttttgtc
     3841 cacctcaaac cttgcataca ggttctgtaa aaactttccc gcctttctaa aatcagctct
     3901 tagttctcca ttccacctgg gtgcagggat cagaggtaaa ttatcactgc cggctttaaa
     3961 cccatcaatc ctttcatcaa acctgccccg aacgaaggaa aagctattct caaaannnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     4081 tgaaacgaaa tgctaaattc tcttctccat ccaccagtac aatagaatca cctcccgcag
     4141 ttgatagtaa tttctgatag aaaataaaat cattcatccg gttaaaaaat gcagaaatac
     4201 ctaaactgat atgttcatag ttcacctcta ccccaccatc cagttgaagg ctggtttctg
     4261 atttcaggtt cagttcgcca tattcaaacc ggtttgttcc ttcatgagta ccattgctgg
     4321 ccagttctgc cagggtaggc gccctgaagc cacgggcaat attggctttt accgttacaa
     4381 aatccaccgg ctcataactc acaccaatac ttccggatat attagagaag ttccgggtga
     4441 atgctgtaaa cttttcttcc gctccttcca tgaaaggctt ggaatcgatg gaacggctgt
     4501 caaaccgaag acctccgctc aatgttcctt ttttgaaaaa acgctgagtg taaacaaatc
     4561 caccaatatc aaagaggtta tactcgggta tcaacacctc ttcgcctttg ttttgattac
     4621 tctgccgcat accatttacg ccaagggttg tatgccattc tttaacgggc agttgccagt
     4681 gcagtgtata gttcagtgtc ttcagatcaa agaatagctc ttcttcatca ggatcttcag
     4741 gattcccaaa ctctttgcga aggttattct gatacgcaag attgatcttt aaacggcttt
     4801 ttttgatggc aaaattgtta tcgcttacga tcttattatg ttgcacccgt tgattaggtg
     4861 caaatacttt tcgtgagtca agatcctccc gggttgcaat tctctcgagg gggctgcctc
     4921 caaaaagaat aaactcaccg gttgcatcat ccctgtctcc ttccactacg cctagttgct
     4981 ggttaaaatg actgaaaata agatggctgt aaccccagct tttattcann nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattaaatct tgaattaagc actttgccat
     5101 cgtatttatt acgatagtca ccggctgatt tgtaagtacc gttgatattc cagttgaatc
     5161 cattattgtt accggcgaga ttggcattag ccgctaataa cccattatta ctttgatagt
     5221 tcatcaatac attgcccttg taagtgccat tggcaacggg tagattcgat ataaattgta
     5281 ttacaccggc caatgcatca ctgccataca ttaatgaagc aggacctttt actacttcag
     5341 cccttgttat actcatttca tcgatttcca gg
//
LOCUS       DAORLS010000216         5364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8367_length_5364_cov_2.019401, whole genome shotgun sequence.
ACCESSION   DAORLS010000216 DAORLS010000000
VERSION     DAORLS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8367_length_5364_cov_2.019401"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>240
                     /locus_tag="PK977_14400"
     CDS             19..>240
                     /locus_tag="PK977_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF19362.1"
                     /translation="MKATKTDVLVIGAGVGGTAAAIQSARLGAKTILVEPTTMLGGML
                     TAAGVSCTDGNDQLPSGMWEEFRQALYKHY"
     assembly_gap    241..381
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <382..1886
                     /locus_tag="PK977_14405"
     CDS             <382..1886
                     /locus_tag="PK977_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF19363.1"
                     /translation="VQKTGNKVTGATLRKIDNSEALKIEATVVIDGTELGDVFAAAGA
                     AYDLGMDDPAVSGEKEAREKNDIIQDLTWAAVLKDYGVGADKTIEKPEGYDAKKFYCT
                     CTDAPCEGKPWNGDKMKMLNYGKLPRSPGAKLDKYMLNWPPFGNDIYLNVVEESSESR
                     VKSYELAKQHTLGFIYFMQTELGLKNIGLADDELDGGMALIPYNREGRRVKGMVRMNI
                     NHIKNPYDNTLYRTGISVGDYPVDHHHARYPGKVPEIEFPPIPAYNIPMGTLIPEKID
                     GLIVCEKGISVTNIVNGTTRLQPVVLLTGQAAGVLATKTVQLKKKVREVPVRTVQDEL
                     LKAKAYLMPFTDVKPDDPHWEAIQKVGATGILKGVGKAEGWANKMFFYPDSLASGAEF
                     SSGMIRFGRYFKSYHRLSRPSGEISLNNCLTFFKRLKPFVKSKTLLAIQSAEDYKNHW
                     IIWGLKDFDPDRALTRREISVVLDKCILLFGSKNLPMDINGKMGFKYSEF"
     gene            1888..2733
                     /locus_tag="PK977_14410"
     CDS             1888..2733
                     /locus_tag="PK977_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRF19364.1"
                     /translation="MRQQLFIACFFFIALSSSAQKDWQLAIQTWTFHKYSFLESVDKA
                     DSLGVRDLEVYPGQKVGGEIPGVFSFTLDKDARDKLKQYLHDKKIRVVALGVIDKYYY
                     NKDNLEKFFEFAKYMNIPFITAEPEWEDLDEFNRLAAKYKVKVALHCHPKPSSHYWHP
                     DSTVKAMQGRKNIGAWPDIGHWARNGVDIQQALIKVKNKLWGLHLKDVEAFDNVKASD
                     VLFGKGICDIPSVFIQLQQMRFKGVLSMEYEANEDNNMEDMRYNKNFVDSLLRQLLHK
                     LQLKQ"
     gene            2787..4154
                     /locus_tag="PK977_14415"
     CDS             2787..4154
                     /locus_tag="PK977_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF19365.1"
                     /translation="MKQVAAASLAAPLASYATISHEEIEKRILPYEKRYSSYDDIQVA
                     IIGFGIMGSRNAKTILQIPGIKLKAVCDLYKGRLERAKELYGNDLFVTQSYEEILNRK
                     DIDAVIICTSDQWHDKISIDAMRKRKAVYCEKPMVHQLNQGWNVINVQKETGVVLQVG
                     SQRVSSIAYAEAKKFYKAGEIGQLNCIEASFDRHTALGAWQYTMPPDASADTVAWKKY
                     FKSSSNLPFDAKRFFWWRNYKEYGTGVAGDLFVHLLSGIHFLTDSKGPSKIFATGDLS
                     YWKDGRNVPDVMTGVMEYKATKEHPAFQVLLKVNLASGAEKVENGKVKFYGTEGMIDF
                     GWNDFTIYRNKFTSFPNIGGWDALDTYPEAMQREIMRSYKQKYPQSEGLQADAKPIRF
                     MAPEGYDDRFDHFVNFFESVRNGKPVVEDAAFGFRAAAPCLACNESYFQKKVINWDPV
                     RMKLI"
     gene            4206..5294
                     /locus_tag="PK977_14420"
     CDS             4206..5294
                     /locus_tag="PK977_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRF19366.1"
                     /translation="MPEQLFSYQQLFDFAKNIFQKIGCSPEHADTAAKTLLSADLRGI
                     DSHGIARLSGYVRLWEVKRVNATPDIKIIHETPSTAVVDGDSGLGLVVAPFAMQVAIE
                     KAKQVGTGWVSVQNSNHFGIAGYHAMMALEHDMIGMAMTNASPLVAPTFSIDKMLGTN
                     PICVAAPAGNEPAFVADLATTTAANGKLEILQRKNADTPLGWVQDAEGNPSTDANILK
                     KGGALLPLGGDREHGSHKGYALGAIVDIFSAILSGANYGPWVPPFPAYVPMPAQQPGK
                     GIGHFLGAMRIDAFRPAEDFKKDMDHWIQGFRKCRTIPGEEKVLVPGDPEKEFEEERS
                     KNGIPLLDAVVDDLKNLADKFFISLLLS"
BASE COUNT         1605 a          916 c         1296 g         1406 t
ORIGIN      
        1 tcagttgcac agcggcaagt gaaggcaacg aaaactgatg tactagttat aggagctggt
       61 gtaggtggca cagctgctgc cattcaatcc gccaggctgg gcgccaaaac aatattggtg
      121 gagcctacaa caatgctggg tggtatgctt acagcagcag gagtaagctg cacagatgga
      181 aatgaccagt tgccaagtgg tatgtgggaa gagttccggc aggctttgta caagcattat
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ncggtacaaa aaaccggaaa caaagtaacc ggcgccacac
      421 tacgaaaaat agataattct gaggcgttaa aaatagaagc aacagtggtg attgatggaa
      481 cagaactggg agatgtgttt gctgcagccg gcgctgctta tgatttgggg atggatgatc
      541 cggcagtgag tggtgaaaaa gaagcaaggg aaaagaatga tatcatacaa gaccttacct
      601 gggctgctgt attaaaagat tatggagtgg gagctgataa aactattgaa aagccggagg
      661 gctatgatgc caaaaaattt tattgtacct gtaccgatgc cccctgcgag ggaaagccgt
      721 ggaatggaga taaaatgaaa atgctgaatt atggaaagtt gcccagaagt cccggtgcaa
      781 agctggataa gtacatgctc aactggccgc cgtttggcaa tgatatttac ctgaacgtag
      841 tagaagagag ttcagagtcg agagtaaaga gttatgagtt agcgaagcaa catactttgg
      901 gcttcattta tttcatgcag actgaactgg gcttgaaaaa tatcggtctt gccgatgatg
      961 aactggatgg tggcatggcc ctgattcctt ataaccggga aggaagaaga gtaaaaggaa
     1021 tggtaaggat gaatatcaac catattaaaa atccgtatga taatactttg tacagaacgg
     1081 gtatatctgt aggcgattac ccggtagatc atcatcatgc ccgctacccg ggcaaagtgc
     1141 cggagataga atttccgcct attcctgcct ataatattcc aatgggaaca ttgataccgg
     1201 aaaaaattga tggtctgatc gtttgcgaaa aaggaatttc tgtgaccaat atagtaaatg
     1261 gtactacaag gctacagcct gttgttttat taaccggcca ggcggcagga gtgctggcaa
     1321 ccaaaacagt tcagttgaaa aagaaagtaa gggaagtgcc ggtgagaaca gtgcaggatg
     1381 aactgctgaa agcaaaagca tacctgatgc cttttaccga tgtaaaaccc gatgacccgc
     1441 attgggaagc tatacaaaaa gtgggtgcta caggaatatt aaaaggagtg ggcaaagcag
     1501 aaggctgggc gaataagatg tttttttatc cggatagttt ggcaagtggt gccgagtttt
     1561 cttctgggat gattaggttt ggccgatact tcaaaagcta tcatcgtttg tcgagacctt
     1621 ctggtgaaat aagcttaaat aattgcctga cattctttaa aagattaaaa ccatttgtaa
     1681 agtctaaaac cttgctggca attcaaagtg cagaagatta caagaaccat tggattattt
     1741 ggggtttaaa agactttgat cctgacagag cattaacaag aagggaaatt agtgttgttt
     1801 tagataagtg tattttactt ttcggcagca aaaacctgcc catggatata aacggtaaaa
     1861 tgggatttaa atacagtgaa ttttaatatg agacaacaac ttttcatagc ctgctttttc
     1921 tttattgctt tatcatcctc agcgcaaaaa gactggcaac tcgccatcca gacatggacc
     1981 tttcataaat acagtttttt ggaatcggta gataaggcag atagtcttgg tgtcagggat
     2041 ctggaagtat atcctggtca gaaagtgggt ggagagatac ccggtgtgtt cagttttaca
     2101 ctagataaag atgcccggga taagctgaag caatacctgc atgataaaaa aatcagggtg
     2161 gtggcattgg gggtaatcga taaatactat tacaataagg ataacctgga aaaattcttt
     2221 gagtttgcaa agtatatgaa tattcccttc attactgctg agcccgaatg ggaggacctg
     2281 gatgaattca accgtttagc tgcaaagtat aaagtgaaag tagctttgca ttgccaccct
     2341 aagccatcca gtcattactg gcatcctgat tctacggtga aagcaatgca aggaagaaaa
     2401 aatatcggcg catggccgga tattggtcac tgggcaagga acggagttga tatacaacaa
     2461 gcattgataa aagtgaaaaa taaattatgg ggtctgcacc tgaaagatgt ggaggctttt
     2521 gacaatgtta aagcttcgga tgtgttattc ggaaaaggga tttgtgatat cccttctgtg
     2581 ttcatacaat tacagcagat gagattcaaa ggagtgcttt ccatggaata tgaggcaaac
     2641 gaggataaca atatggaaga tatgcgctac aacaaaaact tcgttgatag tttattaagg
     2701 caactcttgc ataaactgca attgaagcaa taaaacattc ttttattact taatgcagat
     2761 cgataatgaa aaacaggaga aaatttttga aacaagttgc tgcagcctca ctggctgcac
     2821 cgctggcttc ttatgctacc ataagtcatg aagagattga gaaacgcatc cttccttacg
     2881 agaaaagata ttcctcttat gatgatatac aagtcgccat catcggcttt ggtataatgg
     2941 ggagccgtaa tgcaaagaca attttgcaaa taccgggaat aaagctaaag gcagtttgtg
     3001 atctgtacaa aggccggctg gagagggcaa aagaattgta tggtaatgat ctttttgtta
     3061 ctcaatcata tgaagagatt ttgaacagaa aagatattga tgctgtaatc atttgtacca
     3121 gtgatcaatg gcatgataaa atttccattg atgctatgcg aaaaaggaag gcggtgtatt
     3181 gcgaaaagcc gatggtacac cagttgaacc agggctggaa tgtgatcaat gtgcagaaag
     3241 aaacaggtgt ggtgcttcag gtgggcagtc agcgggtcag tagcattgca tatgctgaag
     3301 caaagaaatt ctacaaagca ggagagatag ggcaactgaa ttgcattgaa gcgtcgtttg
     3361 accggcacac tgctttagga gcatggcaat ataccatgcc acctgatgct tcagcagata
     3421 cggtagcttg gaaaaaatat tttaaatctt cctccaatct gccttttgat gccaaacgtt
     3481 ttttctggtg gcgcaattat aaggaatacg gaacaggtgt agctggcgat ctttttgttc
     3541 atctgttatc tggtattcat tttcttaccg attcaaaagg tccgtcaaaa atctttgcta
     3601 ccggcgatct cagctactgg aaggatggcc ggaatgtgcc tgatgtgatg acgggtgtaa
     3661 tggaatacaa agccactaaa gaacatcctg ctttccaggt attattgaaa gtaaacctgg
     3721 ccagtggtgc agaaaaagta gaaaacggca aagtaaaatt ctatggtaca gaaggtatga
     3781 ttgattttgg atggaatgat tttacaattt accgcaacaa gtttacttcc ttccccaata
     3841 taggcggatg ggatgcactg gatacttacc cggaggcgat gcagcgggaa atcatgagaa
     3901 gttataaaca aaaatacccg cagtcggaag gtttgcaggc agatgcaaag cctatacggt
     3961 ttatggcgcc ggaaggatat gatgaccgct tcgatcattt tgtcaacttt tttgaatcag
     4021 ttcgaaatgg taagccggtt gttgaagatg ctgcatttgg tttccgtgca gcagcgcctt
     4081 gtttggcttg taatgaaagt tattttcaaa aaaaggttat taactgggac cctgtcagga
     4141 tgaaacttat ttgatttttg aagttttgaa attcccttta ggacgttgta ggttatcttt
     4201 gcccaatgcc agaacaactg ttttcttacc agcaattatt tgattttgca aagaatatct
     4261 ttcaaaagat cggctgctcg cctgagcatg ctgatacagc tgccaaaacc ttattgtctg
     4321 ctgatcttcg tggaatagat tcacatggta ttgcccgcct cagcgggtat gtaagattat
     4381 gggaggtaaa aagagtcaat gcaacacctg atattaagat cattcatgaa actccttcga
     4441 ctgcggtggt agatggtgac agcggattag gattggtggt agcacctttt gccatgcagg
     4501 ttgctattga aaaagcaaaa caggtgggta ccggttgggt aagtgtacaa aatagcaacc
     4561 actttggcat tgccggttat catgcaatga tggcactgga acatgatatg atcgggatgg
     4621 cgatgaccaa tgccagtccg ctggtagcgc caactttttc tattgataaa atgttgggta
     4681 caaatcccat ttgtgtggct gcacctgcgg gtaatgaacc tgcatttgtt gctgatcttg
     4741 ctaccaccac tgcggccaac ggtaagttgg aaatattgca acgtaaaaat gcagatacac
     4801 ctttgggctg ggtgcaggat gcagaaggaa atccatccac ggatgcaaat attttaaaga
     4861 aaggcggtgc attactgccg ctgggtggcg acagggagca tggttctcac aaaggttatg
     4921 ctttgggtgc tattgtagat attttttcag ccatattaag cggtgcaaat tatgggccat
     4981 gggtaccgcc gtttcctgct tatgtgccta tgccggcaca acaaccggga aaaggaatcg
     5041 gtcatttcct tggtgctatg cgcattgatg cattcaggcc ggctgaggat tttaaaaaag
     5101 acatggatca ctggatacag ggattccgga aatgcagaac cattccggga gaagaaaaag
     5161 tgctggtgcc gggtgatccg gagaaggagt ttgaggaaga gagaagtaaa aatggtatac
     5221 cattgctgga tgctgttgtg gatgatctga agaaccttgc agacaaattt tttatatctt
     5281 tacttctgtc atgaaaacat tttttcaaat tcttaaactt ttgttcctta taccttattt
     5341 tgtttcatgt caaaaagaaa tagg
//
LOCUS       DAORLS010000217         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8439_length_5327_cov_1.744879, whole genome shotgun sequence.
ACCESSION   DAORLS010000217 DAORLS010000000
VERSION     DAORLS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8439_length_5327_cov_1.744879"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK977_14425"
     CDS             <1..315
                     /locus_tag="PK977_14425"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRF19367.1"
                     /translation="FAIRSMSSGNAVMGFDLTGANTKLAIYYRYNKNVQIDTTVTYFS
                     FSGNFCAAANYIQRDYSGTPLETALGGAAPDHVVYIQNTPGTFAKLKIPECDQQQAHP
                     PG"
     gene            260..769
                     /locus_tag="PK977_14430"
     CDS             260..769
                     /locus_tag="PK977_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19368.1"
                     /translation="MQSLRYLNVINNRLIHRAELIVEQLYDISDSTFYSPGLLYLDAS
                     DPTITSPYKFRTIPYDLSFTASGALNLGEFGCAPVISTDGAGNKIRVWKFNISRYVQH
                     VLTRTQSLYDLRLFAPFSINEKYGIPPGTDVTATIFVNTTTTKGRIRVGGGNHPSQKM
                     RLRLVYSKL"
     gene            872..>1856
                     /gene="metK"
                     /locus_tag="PK977_14435"
     CDS             872..>1856
                     /gene="metK"
                     /locus_tag="PK977_14435"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRF19369.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAFDPQSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQEIARGVIRKIGYTKSEYMFEASSCGVLSAIHEQSADI
                     NQGVDKKKKEEQGAGDQGMMFGYATNETEHYMPLALDLAHNLLIELAAIRREKNSKMP
                     YLRPDAKSQVTLEYDDNNRPVRIDAIVISTQHDDFDSETKMLAKIKSDVINILIPRVK
                     NKYKKYAKLFNNDIKYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGLCDEVLVQVSYAIGVAQPTSINVNTYG"
     assembly_gap    1857..1918
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1919..2122
                     /locus_tag="PK977_14440"
     CDS             <1919..2122
                     /locus_tag="PK977_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRF19370.1"
                     /translation="YFIEQRLKLRNPIYSETAAYGHMGRTPQVVEKTFVSPDGKKVTK
                     KVELFTWEKLDYVNKVKKAFGLK"
     assembly_gap    2267..2276
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2277..2962
                     /gene="rlmB"
                     /locus_tag="PK977_14445"
     CDS             <2277..2962
                     /gene="rlmB"
                     /locus_tag="PK977_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRF19371.1"
                     /translation="AGPQLDRIYLDTKAQGQDISDIKRLASQQGVPVNYVPAAKLNGF
                     NITDHEGCVAQIAKVQYQNLQDVISLVIESGKPPLFLLLDGITDIRNIGGIARTAYCT
                     GVDAIIIPDKGVGALNEDAILTSAGALEKIAVCRVNSLMKAVDELHLNGIKVYASDMT
                     AEKNIAEVDFTEPCAIVMGSEEKGIYPALMKICDEIIKIPMAGDFESLNVSVATGIKL
                     YEAMKQRMQ"
     gene            2959..>3623
                     /locus_tag="PK977_14450"
     CDS             2959..>3623
                     /locus_tag="PK977_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRF19372.1"
                     /translation="MSGYNKIKLIECPRDAMQGWKSFIPTEKKAAYINSLLRVGFDTI
                     DFGSFVSPKAIPQMADTVEVLKSLEVEKSVSKLLAIIANYRGAEAAVVFDEITYLGFP
                     FSVSETFQQRNTNSSITESLGRVEEIQSLCIKSGKQLVVYISMGFGNPYGDLYDEEIV
                     FEWVNKLVGMDIGIISLADTVGVATAQQVYDMTSYLVESLPGTEIGVHLHSTPHNWKE
                     KLEA"
     assembly_gap    3624..3721
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3893..>4740
                     /locus_tag="PK977_14455"
     CDS             3893..>4740
                     /locus_tag="PK977_14455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRF19373.1"
                     /translation="MVHIDLKKLSEPITYGDKIMLMGSCFTEHIGNELADLKFDVLQN
                     PNGILFGPDAVCKSLLSYIVNKHYTGDDLFYLNEVWNSWDHHSRFSHINKDECLQGIN
                     QSQQKAHDFLKEADWLIITLGSSFSYFLTSQPSFDKSRGGEAVANCHRAPANWFNKEM
                     LEIDTIKEMLVSCLEKLKAFNPRLKFIFTISPVRHIRDGVVENNRSKARLIEAVHHIA
                     GKSESAFYFPAYELVIDVLRDYRFYDIDMVHPNYPATEFVLEKFSDTCIAEGSQQLMK
                     EVKQII"
     assembly_gap    4741..4750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4881..5120
                     /locus_tag="PK977_14460"
     CDS             4881..5120
                     /locus_tag="PK977_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19374.1"
                     /translation="MWLVTIVFWLQAFAAPVILFGLIGLAIGNETAFYVLLGTGILTG
                     LIVAEYIRRKIGLDTFFGRIYGPNEMDKKAGKKTK"
     gene            complement(5113..>5327)
                     /locus_tag="PK977_14465"
     CDS             complement(5113..>5327)
                     /locus_tag="PK977_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HRF19375.1"
                     /translation="VHSTEPVVHFYSGKWIPVVKGKNGITYGAFSGLCLETHKHPNAI
                     NIQAFPNTVLRPGEVYSSRTTYKVII"
BASE COUNT         1501 a          979 c         1203 g         1464 t
ORIGIN      
        1 ttcgccattc ggtctatgag ttctggtaat gcagtgatgg ggtttgatct tactggcgct
       61 aataccaaac tggctatcta ttatcggtat aataaaaacg ttcaaataga tactacggtt
      121 acatatttta gtttttccgg caatttctgt gctgctgcaa attatattca aagggattat
      181 tcgggcacgc cacttgaaac tgccctggga ggagctgcac ctgatcatgt tgtttatatt
      241 cagaatacgc cgggaacatt tgcaaagctt aagatacctg aatgtgatca acaacaggct
      301 catccaccgg gctgagctga ttgtggaaca gttgtatgat atcagtgaca gtacttttta
      361 cagtcccggg ctactatacc ttgatgcaag tgaccctaca attacttcac cctataaatt
      421 cagaaccatt ccttatgatc tttcattcac ggcatcgggt gctttaaatc ttggtgaatt
      481 tggctgtgcc ccggttatca gtacagatgg tgccggtaat aagatacggg tatggaagtt
      541 caatatttcc cgctatgtgc agcatgtact tacccgtacg cagagtttat atgacctgag
      601 actttttgct cctttttcta tcaatgaaaa atatggtatt ccgcctggta ctgatgttac
      661 tgccaccatt tttgtaaata ctaccactac aaaaggaagg atcagagttg gcggtggtaa
      721 tcacccgtca caaaagatga gattacggtt agtatattca aagctttgat attctattta
      781 tcaagatatg ttcaaaagtc cttgtgtgat agcacaagga ctttgttctt tataagcatg
      841 gcccttatct ttgcggccta aattcatatt catgccttat ttatttactt cagaatctgt
      901 aagtgaagga cacccggata aagtagcaga ccagatatct gatgccctta ttgataattt
      961 tctggccttt gacccccagt caaaagttgc ctgcgaaaca ttggtgacta ccggacaagt
     1021 ggtattggca ggtgaggtga aatcaaaagc ctatttagat gttcaggaaa ttgcccgtgg
     1081 tgtgatccgt aaaattggtt ataccaagag tgaatatatg tttgaagcca gcagttgcgg
     1141 tgtgctttcc gcaattcatg aacaatcagc tgacatcaac cagggtgtcg acaaaaagaa
     1201 aaaggaagaa cagggagccg gcgaccaggg catgatgttt ggctatgcta ccaacgagac
     1261 agagcattat atgccattgg ccttagacct ggcacataac ctgttgattg agctggcggc
     1321 tatccgcagg gaaaaaaact caaaaatgcc ttacctgcgc ccggatgcaa aaagccaggt
     1381 gactcttgaa tacgatgata ataacaggcc ggtgagaatt gatgctatcg ttatctccac
     1441 acagcatgat gatttcgatt ctgagactaa aatgctggct aaaatcaaga gtgatgtgat
     1501 caacatcctt atcccaaggg tgaagaacaa gtataagaag tatgctaaac tgttcaacaa
     1561 cgatattaaa taccatgtga accctaccgg aaagtttgtg atcggtggtc cgcatggtga
     1621 tacaggttta accggcagaa agatcatcgt tgatacatat ggtggtaaag gagcacatgg
     1681 cggcggcgct tttagcggaa aagatccttc gaaggttgac cgttctgctg cttatgctac
     1741 aaggcatatt gccaagaacc tggttgcggc cggtttgtgc gatgaagtac tggtacaggt
     1801 ttcttatgct attggtgtag cccagccaac cagtattaat gttaacactt atggcannnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     1921 ttttattgaa cagcggctga aattgcgcaa tcctatttac agcgaaacag ctgcttatgg
     1981 acatatgggc cgtacaccgc aggtagtgga gaagacattt gtttcgcctg acggcaaaaa
     2041 agtaacaaag aaagtagaat tgtttacctg ggagaaactg gattatgtga acaaggtcaa
     2101 gaaagcattc ggtcttaaat aatatgaagc cctctccctg agagggtttt ttgtttgcct
     2161 acatttgtat agtgaaaaat ttcagacaac aacaacggcc caagaaaagt gccatggtta
     2221 tcgggcggca gccagtagct aatgccttaa aaaacggaca gcagctnnnn nnnnnnaggc
     2281 tggcccgcag cttgacagaa tttacctgga cacaaaggcg caggggcaag acatcagtga
     2341 tataaaaaga ctggcttctc agcagggtgt tcctgttaat tatgtaccgg cagccaaatt
     2401 aaatggcttt aacatcactg atcatgaagg ctgtgtggca caaatagcca aagtgcagta
     2461 tcaaaacctg caggatgtca tttcattggt tattgaaagt ggcaagccac cacttttcct
     2521 gctattggat gggattactg acatccgtaa catcggcggt attgcccgca ctgcttattg
     2581 taccggtgta gatgccatta ttatcccgga taaaggagta ggagccttga acgaagatgc
     2641 gatacttact tctgccggcg ccctggaaaa aatagctgtt tgccgtgtta atagtttaat
     2701 gaaagcagtg gatgaactgc acctgaacgg gataaaggtt tatgccagcg atatgacagc
     2761 agagaagaac attgcggagg ttgattttac tgaaccttgt gccatagtaa tgggcagtga
     2821 agaaaaaggt atttacccgg cactgatgaa gatttgtgac gagataataa agatcccgat
     2881 ggctggtgat tttgaatcgc tgaatgtttc tgttgcaaca gggataaaac tttatgaagc
     2941 aatgaaacag aggatgcaat gagcggatat aacaaaataa aactcatcga atgtccccgt
     3001 gatgccatgc agggctggaa atcttttatc cctactgaaa agaaggcagc ctatatcaat
     3061 tcgttgctca gggttggttt tgataccatt gattttggca gttttgtttc acccaaagcc
     3121 attccgcaga tggctgacac agttgaggtg ttaaaaagtt tagaagttga gaagtcggta
     3181 agtaagttat tggctattat cgccaattac cggggtgcag aagctgctgt tgtgtttgat
     3241 gagataactt atcttggttt tccgttctcc gtttcagaaa cgtttcagca aaggaatacg
     3301 aattcatcca taactgaatc gctgggcagg gtagaggaga tacagagtct ttgtatcaaa
     3361 tcaggaaagc agttagtggt ttatatatcc atgggttttg gaaatcctta tggcgacctg
     3421 tatgatgaag agatcgtttt tgaatgggtg aataagctgg tcggaatgga tatcggtatt
     3481 atttccctgg ctgatacagt gggtgtagct accgcccaac aggtgtatga tatgaccagt
     3541 tacctggtcg aatctttgcc cggcaccgaa atcggagtac acctgcattc taccccgcat
     3601 aactggaaag aaaaattaga agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 ngcgaatgga tgataaatta ttttgaagaa aatatgctgc aggatgattt gaacaaagaa
     3781 gcattgcagg aaagcctcaa aatggcaggt gaaatatttg tataacaggt attctgcaag
     3841 aacctttttt aatttttgaa ctaattaaag tatcaatttg gcaatggatt tcatggtaca
     3901 tattgatctg aaaaaattgt cggagccaat aacttacggt gataagatca tgctgatggg
     3961 ctcctgtttt accgagcata tcggtaatga gctggctgac ctgaagtttg atgtgttgca
     4021 aaaccccaat ggtattttat ttggccctga tgcagtttgc aaaagtctgc tgtcatacat
     4081 tgtcaataag cactacaccg gggatgattt gttttacctg aatgaggtgt ggaacagttg
     4141 ggaccatcat tcgagattct ctcatatcaa taaagatgaa tgcctgcagg ggataaacca
     4201 atcacaacaa aaagctcatg attttttgaa agaagcagac tggttaataa tcacacttgg
     4261 ttcttccttt agctattttc tcacttccca accttctttt gataaatcga gagggggaga
     4321 agctgtggct aattgccacc gggctccggc taactggttc aataaagaaa tgctggagat
     4381 tgatacaata aaggagatgc ttgtaagttg cctggaaaaa ttaaaagcgt tcaacccccg
     4441 gctgaaattt attttcacta tcagtcctgt gcggcatatc agggatggag tggttgaaaa
     4501 caaccggagc aaggcaaggc tgatcgaagc ggttcatcat attgccggca aatctgaatc
     4561 tgccttttac tttcctgctt atgaactggt gatcgatgtg ctaagggatt accgttttta
     4621 tgatattgat atggtgcatc ccaactaccc ggcaacggaa tttgtactgg aaaaattttc
     4681 agacacctgc attgccgaag gatcacaaca attgatgaaa gaagtaaagc agatcatcat
     4741 nnnnnnnnnn cttttcagcc ggaaacaaag gcgcaccagc aatttttggc ggcacatttt
     4801 gaaaaagcaa aattgctgca ggataaatat ccttttcttg acctgaaaga agagctggtt
     4861 tatttctcac aagtataatt atgtggcttg ttacaatagt attttggctg caggcatttg
     4921 cggcgccggt cattcttttt ggattgattg gtttggctat cggtaatgaa acggccttct
     4981 atgtgttatt aggtacaggt attttgaccg ggctaattgt tgcagaatac atacgcagaa
     5041 agatagggct tgataccttt tttggtcgta tttatggccc caatgaaatg gataaaaagg
     5101 cgggcaaaaa aactaaatga tcactttgta ggttgttcgg ctgctgtaca cctcaccggg
     5161 tcttaaaacc gtattcggga atgcctgaat atttatcgca ttgggatgtt tatgtgtttc
     5221 cagacacagg cctgaaaacg ctccgtaagt aataccgttt tttcccttca cgacgggtat
     5281 ccatttgccg gaataaaaat gtactaccgg ttcggtactg tgtactt
//
LOCUS       DAORLS010000218         5321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8453_length_5321_cov_2.138625, whole genome shotgun sequence.
ACCESSION   DAORLS010000218 DAORLS010000000
VERSION     DAORLS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8453_length_5321_cov_2.138625"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PK977_14470"
     CDS             complement(<1..741)
                     /locus_tag="PK977_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF19376.1"
                     /translation="MSTRILITGGTGFLGSYIIKELVEKGYAVRALRRSNKLPAWIPS
                     EILEKTEWVDGDVLDVVALEDAMEGVDVVIHAAAIVSFVKKDREQMYQVNVEGTANVV
                     NTALEKNVRRLVHISSVAALGRTTNGGHVDEEKKWEENKANTHYAKSKFKAELQVWRG
                     ISEGLEAVILNPSTILGYGDWHSSSCAIFRQVYEGFKWYTPGINGFVDVEDVARATVL
                     LMESNISEQRFIVNGDTWPFQKLQNTIAD"
     gene            complement(813..1661)
                     /locus_tag="PK977_14475"
     CDS             complement(813..1661)
                     /locus_tag="PK977_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRF19377.1"
                     /translation="MEINSICVCGAGTMGSGIAQAAAQAGFATLLYELNPVVLDKARE
                     NISKNLQSLVNKGKMEAAEKEKIEKRIGFTDDIQNCLADIFVEAIIEKTDIKVALFNQ
                     LAELNHSECIFASNTSSLSITAIAKNVQRPERVIGMHFFNPATIMKLVEVVNTEYTNQ
                     QTTATVVELAKQMGKIPVVCKDSPGFIVNRVARPYYIEALRLVEEGKVSMEQADAIME
                     ASGFKMGPFKLMDLIGNDVNYAVSCSVYEQLGQPERLKPSYIQEQKVKEGKLGKKTGE
                     GYYKYS"
     assembly_gap    1694..1714
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1715..2388
                     /locus_tag="PK977_14480"
     CDS             <1715..2388
                     /locus_tag="PK977_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19378.1"
                     /translation="CRNSYLYYLSASKTKTTFMETTQPVSNPVYPPAPPSPGIFGSKV
                     PSSVSFAVVLLLFLLPLSEIRCAGTALMSKSGLAFATGKEWTFMGGMGKDMMKDMKAP
                     KKGEKEGNSQYFIIGAAALALLGLLLALAGNKTTGYAATACGVLGAGALIGFMLDLKK
                     WFDAGLAKQAAEKTKEGSDSIGLDNMDSLTNVTLGFTPWFYIAVIAFLLAALFSYKRS
                     ASLKP"
     gene            2433..2834
                     /locus_tag="PK977_14485"
     CDS             2433..2834
                     /locus_tag="PK977_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19379.1"
                     /translation="MNRKTITMILAVVIIAAFFLPYFSLGGLLKMSGLDIITGGSMGA
                     GSGGSADRFVLLLTPIAGVLLLVGALNNEKYTPSRSVLGILALVGWIYPLIRGLMEGG
                     SGMSQIFKFMGIGFWLGLVAALAVILYNPKS"
     gene            2915..3826
                     /locus_tag="PK977_14490"
     CDS             2915..3826
                     /locus_tag="PK977_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19380.1"
                     /translation="MLKKSIFAFSILVSLQATTQTYDQWAQTVGWDGISHWKKYMYTQ
                     PAYMGPNALPVPRLGNSSIDSSFFIAVTGMLHFSKGDNTQNLSVYANYCLVKDLISFD
                     AVWVPYERYNMSLATKQERHVFAQFFYDKEATGEVHLNTNLQLLNKWRKHIHLVLRLG
                     YRFPCSSGFGAARYSDGPGYYFDLSFGKTLGKNLKWNGMTGFYSWQIQSDKHQQDDAF
                     LFGTGLEWNQNNWRIQTNVAGYLGYLKNSGDKPVVYRFNLEKRIKRTGVLLAFQQGLH
                     DFDYSTIEFGAKYYLNRKSLSLAKLPK"
     gene            complement(3810..>4874)
                     /gene="pheS"
                     /locus_tag="PK977_14495"
     CDS             complement(3810..>4874)
                     /gene="pheS"
                     /locus_tag="PK977_14495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRF19381.1"
                     /translation="NSFIPLSYLCSLMEQLLNKIEAYKNEASSTVATDTKTVEEFRIK
                     WLGTKGIVKEIMGEMKNVAPEKKKEAGQLLNEFKQFVEARYAELKDANSTTEHQTSNI
                     DLSLPGDELPIGSRHPVTLMRNRIVSIFQRLGFAVAEGPEIEDDWHNFGALNLPEHHP
                     ARDMQDTFYIQQNPDWVLRTHTSSVQIREMEKGNLPIRMLMPGRVYRNETVSARSHCF
                     FHQVEGLYIDENVSFADLKQTLYFFVKEMYGKDVKVRFRPSYFPFTEPSAEMDVSCFI
                     CGGDGCNICKKTGWVEILGCGMVHPNVLSNCGIDPNKYTGFAFGMGIERPAMMKYGIN
                     DIRLLSENDVRFLKQFTSAI"
     assembly_gap    4875..5057
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5058..>5321
                     /locus_tag="PK977_14500"
     CDS             <5058..>5321
                     /locus_tag="PK977_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRF19382.1"
                     /translation="DAYQEHFPDLDEAQIVLVGCGEQRGSGLIHGHSEAPDIIRRHFY
                     SLFYWHTDIKVADVGNIKAGSLFTDSYAALKTVVQELIGDGKTV"
BASE COUNT         1354 a         1151 c         1063 g         1549 t
ORIGIN      
        1 atcagcaatc gtattctgta atttttgaaa tggccatgtg tcaccattta caataaatct
       61 ttgttctgat atattgcttt ccatcagcaa aacggtagcc cttgcaacat cttctacatc
      121 tacaaaacca ttaatgcccg gggtgtacca tttaaaacct tcgtaaacct gcctgaaaat
      181 ggcacaactg ctgctgtgcc agtcgccgta acccagaata gtactcggat ttaatatcac
      241 tgcttctagt ccttcactaa tgcctctcca tacctgtagt tcagctttga atttgctttt
      301 ggcataatgg gtattggcct tgttctcttc ccattttttt tcttcatcta catgcccgcc
      361 attagtggtt ctgccaaggg cagctaccga actgatatgc accaaacgtc gtacattttt
      421 ttccaaagct gtattcacaa cattggctgt tccttcgaca tttacctggt acatctgttc
      481 cctatctttt tttacaaaag aaacgatggc ggcagcatga ataacgacat caaccccttc
      541 catcgcatct tccagcgcca ctacgtccaa tacatcacca tccacccatt ctgttttttc
      601 aagaatttcg gaaggaatcc atgccggtag tttattactg cggcgaaggg cccttacagc
      661 atagcctttt tctaccagtt ctttaatgat ataggaacct aagaaaccgg taccgcctgt
      721 aataagtatc ctggttgaca agttgattct tttaaagagg ctgcaaattt acaagtgcta
      781 tttcctttta tacttgtaaa cctgaaaacc tgttaactgt atttataata gccttcgcct
      841 gttttctttc ccagttttcc ttcttttact ttttgttcct gaatatagga gggcttcagt
      901 ctttcaggct gcccaagttg ctcgtaaaca gaacaactca ctgcatagtt tacatcatta
      961 ccgatcaggt ccatcagttt gaatggcccc atcttaaaac ctgatgcttc cattatagca
     1021 tctgcttgtt ccatactcac tttgccttct tccaccagcc tcagggcttc aatataataa
     1081 ggccttgcta cccggttcac aataaagcct ggagaatctt tgcagactac agggatttta
     1141 cccatttgct ttgcaagttc cacaacagtt gcagtagttt gttggttggt atattccgta
     1201 ttaaccacct ctaccagttt catgatagtg gcagggttga aaaaatgcat accgatcacc
     1261 ctttccggcc gctgcacatt tttggcaata gcggtgatgg aaagagatga agtattgctg
     1321 gcaaaaatgc attcactgtg gttcagttct gccagctggt tgaataaagc gaccttgata
     1381 tctgttttct caatgattgc ctcaacaaaa atatcagcga gacaattttg tatatcatct
     1441 gtgaagccga tccttttttc aatcttttct ttttctgcag cttccatttt ccctttatta
     1501 accaatgact gcaggttttt gctgatgttt tcccttgctt tatcaagtac aacagggttt
     1561 agctcataaa gcaaagtagc aaacccggcc tgagcagcag cctgagcaat gccactgccc
     1621 atagtgccgg ctccgcaaac gcatattgag tttatttcca ttagaaaatt tacggctgta
     1681 aagatattgg ttgnnnnnnn nnnnnnnnnn nnnngttgca ggaatagtta tttatattat
     1741 ctttctgctt ctaaaaccaa aaccactttt atggagacta cccagcctgt ttctaatcct
     1801 gtttatcccc ctgcacctcc gtcacccgga atatttggat ccaaagtacc ttcttccgtg
     1861 tcatttgccg ttgtactatt actgtttctg ctgcctttat cagaaatacg ttgtgcaggc
     1921 actgctttga tgagtaagag cggacttgcc tttgcaaccg gaaaagagtg gactttcatg
     1981 ggcggcatgg gaaaggatat gatgaaggat atgaaggcac ctaaaaaagg tgagaaggaa
     2041 ggcaattcac agtactttat cattggcgct gcagcgctgg ctttgttagg tcttttgctg
     2101 gctcttgcag gtaacaaaac aacaggttat gcggctacag cctgcggcgt actgggtgcc
     2161 ggtgcattaa ttggctttat gctcgacctg aaaaaatggt ttgatgcagg attggccaag
     2221 caagcagccg aaaaaaccaa agagggatcc gattccatcg ggcttgataa catggacagc
     2281 cttaccaatg ttacccttgg gtttactcca tggttttata tagcagtcat tgctttctta
     2341 ctggcagcct tattcagcta taagcgatcg gcatcattaa aaccctgatt cattttacaa
     2401 gcatcatcat tattcaccat aaaccaacca acatgaacag aaaaacaatc acgatgattt
     2461 tggcagtagt aattattgcc gcattctttc tcccttattt tagcctggga ggacttttaa
     2521 aaatgagcgg tcttgatatt attactggtg gcagcatggg cgcaggtagt ggaggctcag
     2581 cagacagatt tgtattattg ctgacaccca ttgccggtgt gttgttactg gtaggtgctc
     2641 tcaacaatga gaaatatact cccagcagat cagtgttagg tattttggcc ctggtaggct
     2701 ggatctatcc gttgatccgc ggattaatgg aaggcggttc aggtatgagc caaattttca
     2761 aattcatggg catcggcttt tggctgggcc ttgtggcagc cctcgctgtt atcctttaca
     2821 atccaaaatc ataatcacag ttcataggtt tttttaaggc tgccaccggg cggccttttt
     2881 ttgtgcctgc cgcaaatttt aactttgaac agtcatgctg aaaaaatcaa tttttgcttt
     2941 ttcaatactg gtgtctttgc aggcaactac acaaacttat gaccaatggg cacaaacagt
     3001 gggctgggat ggtataagcc attggaaaaa atatatgtac acacagccgg cttacatggg
     3061 gcctaatgca ttgcctgttc cccggctggg aaacagcagc attgacagca gtttttttat
     3121 agctgttacc ggcatgcttc atttctcaaa aggagataat acacaaaacc tttctgttta
     3181 tgcaaactac tgcctggtaa aagatctgat ctcatttgat gcagtatggg tgccctatga
     3241 acgttacaac atgagccttg ccaccaaaca ggaacgtcat gtgtttgccc agttttttta
     3301 tgataaggaa gcaacaggag aagtacacct gaatactaat ctccaattgt taaataaatg
     3361 gcgaaaacat attcacctgg tattgcggtt aggctatcgg tttccctgtt catcaggctt
     3421 cggcgctgcc cgttattctg acggaccggg ttattacttt gatctgtcat ttggcaagac
     3481 attgggaaaa aacctgaaat ggaatggtat gacaggtttt tattcctggc agatacaatc
     3541 tgataaacac cagcaggatg atgctttctt atttggaacg gggctggagt ggaatcaaaa
     3601 taattggcgc atacaaacca atgtggccgg ttacctgggc tatttgaaaa acagcgggga
     3661 caaaccggtc gtataccggt ttaatttgga aaaaagaata aagagaacag gtgtattact
     3721 cgcatttcag caagggctgc atgattttga ttacagcaca attgagtttg gcgccaaata
     3781 ctatctcaac cggaaatctt tatccttagc taaattgccg aagtgaactg cttcaggaac
     3841 cgtacatcat tttcacttag caggcgaata tcattgatac cgtatttcat catggcgggt
     3901 ctttcgatac ccataccaaa agcgaagccg gtatatttat tcggatctat gccacagttt
     3961 gataacacgt tgggatgtac catgccacag cctaagattt ctacccaacc tgtctttttg
     4021 cagatgttac agccatcgcc gccgcaaata aagcagctta catccatttc agcactgggt
     4081 tcggtaaaag gaaagtaaga aggacggaaa cgaactttta catccttgcc gtacatttct
     4141 tttacaaaga agtataatgt ttgcttcagg tcggcgaatg aaacattttc atcaatatat
     4201 aaaccttcta cctggtgaaa gaaacaatga cttcttgcac tcaccgtttc gttacggtaa
     4261 acacggcctg gcatcagcat acgtattggc aggttgccct tctccatttc ccgtatctgc
     4321 acactgctgg tatgcgtacg caacacccaa tccgggtttt gctgaatata aaaagtgtcc
     4381 tgcatgtcac gggcaggatg gtgctccggt aagttcaggg caccgaaatt atgccagtcg
     4441 tcttcaattt ctggtccttc ggcaacagca aagccgagac gctgaaaaat agaaacgatc
     4501 ctgttccgca tcagtgtcac cgggtgacgg gaaccgatag gtagctcatc gccgggcagg
     4561 ctcaaatcta tgtttgatgt ttgatgttct gtagttgaat ttgcatcttt caattcagca
     4621 taccgggctt caacaaattg tttaaactca ttcagtaact ggccggcttc ttttttcttt
     4681 tcgggggcta catttttcat ctcacccatg atctctttta ctataccttt tgtgcccagc
     4741 catttaatgc ggaattcctc aacagttttt gtatctgttg caacggtact gctagcctca
     4801 tttttatacg cttcaatctt gttcagcaat tgttccataa ggctgcaaag ataagataag
     4861 ggaataaagg agttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnngat gcctaccagg aacattttcc ggacctggac gaagcacaga
     5101 tcgttttggt cggttgcggg gagcagcggg gaagcggatt gatccatggc catagcgaag
     5161 cccctgatat tatccgccgt catttttatt ctttgttcta ctggcatacc gatataaaag
     5221 tggccgatgt gggcaatatt aaagccggct ccctgtttac cgattcttat gcggcattaa
     5281 aaacagtagt gcaggaactg atcggagatg gaaaaacggt g
//
LOCUS       DAORLS010000219         5312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8466_length_5312_cov_1.994864, whole genome shotgun sequence.
ACCESSION   DAORLS010000219 DAORLS010000000
VERSION     DAORLS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8466_length_5312_cov_1.994864"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>214)
                     /locus_tag="PK977_14505"
     CDS             complement(<1..>214)
                     /locus_tag="PK977_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19383.1"
                     /translation="ENVKKLREIYKQEDTILSKVLHEAERLEGSVRNTGIHAAGIIIA
                     PKDLTELIPVATSKESNLWLTQIEGNV"
     assembly_gap    215..369
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<370..577)
                     /locus_tag="PK977_14510"
     CDS             complement(<370..577)
                     /locus_tag="PK977_14510"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19384.1"
                     /translation="MKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIDYVVEKYGKN
                     QVAQIITYGTMAAKSSIADVARVMD"
     assembly_gap    580..693
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<694..1840)
                     /locus_tag="PK977_14515"
     CDS             complement(<694..1840)
                     /locus_tag="PK977_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRF19385.1"
                     /translation="MKFSHLHVHTQFSLLDGAASIQNLYKKAIKDGMPALAISDHGNM
                     FGVFEFVAEAYKNKGADGKPIVKPIVGCEFYITADRHQKAFTKDQKDPRHHQILLAKN
                     EEGYRNLVKLTSLGFIEGMYSKYPRIDKELILRYHKGLIATTCCLGAMVPQAILKKGE
                     EEGENEFKWWLNIFQEDYYVELQRHGMAEQDRVNEVLIKLAKKYNVKIIASNDSHYVE
                     QDDFNAHDILLCINTGEKITTPALREFSDDDVHVKDKRFAFPNDQFYFKTTEEMSKAF
                     NDLPEAIDNTNEIVDKVELLNLHRDILLPNFPIEKRFQVHTKEEIIGKNVISPEALNQ
                     WEYLKHLTYEGAKQRYSELTDEIKERIDFELFTIKTMGFAGYFLIVGD"
     gene            complement(1853..3067)
                     /locus_tag="PK977_14520"
     CDS             complement(1853..3067)
                     /locus_tag="PK977_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRF19386.1"
                     /translation="MQTLFSLLSLYFLAIGCQTSGNSKKPDIETLFSKHVNADTIPPA
                     PKLKPYSSIEYEKKEVLTAAGGTKSVIFNSSGSKLYAMNLEGMSVYEFDQASRKISRE
                     FKFTPTRGTGWDYNRSRPIASFQEKPVEACFSHDDKILWISLHNADGIVPIWVDDYSK
                     NNAVTTAEQKTKPVTVIYPGTAKKDSFRVPLIETGKTPKVISRTADSKYLLVSNWHSR
                     NTSVLEMDETKLPYAKVISTIPVSAIPRGIAVDDKNKKSYIAIMGGSSLNVVDNTTWK
                     SESIMNVWSSPRHMVMDTSGHLFVSYNSLGTIACIDAATGQQLFTAKTHAQPRTIVLS
                     KNHKFIFVTCYTSDYVDVYKINNDNFEKVASLSCGGHPVGVDIFENDDKLEAWVCSYS
                     SGSISIYSFKKK"
     gene            3329..>3730
                     /locus_tag="PK977_14525"
     CDS             3329..>3730
                     /locus_tag="PK977_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19387.1"
                     /translation="MVRQILPAVAAVIFLSSCSTFKSLNFTSNKQVTTTVQQLDAPRT
                     KFIDEIAVTPVAAQQQLKLEKAEPKSGPAASISDRLVNIPEPIKQEVAQPEKSIVTRE
                     AEIESASEVQFKYAILMNTEVESLPSKQLLET"
     assembly_gap    3731..3747
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3748..4101
                     /locus_tag="PK977_14530"
     CDS             <3748..4101
                     /locus_tag="PK977_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRF19388.1"
                     /translation="GVRYRTGGNTKSGVDCSGFTVAVYSAVFGIMLPRVSREQYRISR
                     KISTTELQQGDLVFFNTNGRGVSHVGVYLGNNKFIHASVSKGVMVNDLFEPYYLKRYI
                     GAGRIDDKQMVASGN"
     gene            complement(4106..>5312)
                     /locus_tag="PK977_14535"
     CDS             complement(4106..>5312)
                     /locus_tag="PK977_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRF19389.1"
                     /translation="IAGWTLKHLVIKKFGTNTPQLVAYYYWIFPLGLGLTIYTVLEAY
                     AWSLHKSVLTNFFREVQWRLFTTVLIVLFISNIIKDYDLFIKIFAFSYPSIALSLLAY
                     LVITKKIHFTFTVSKVSRRYFKKILALCSFVYGGTLIFTISQVFDTIVIASLLEEGSA
                     KAGIFGLATIVTSIIQAPQRGIISASIPHLSKAWKDKNMALLQKVYQRSSINLLIFAC
                     GIFLLIWLNFTDGIITFGVKPSYLDAAWIFFLLGITKIIDMGTGVNSQIIGTSTYWRF
                     ELISGVILLSVMLPLTYILTKQYGLIGPAIAALISISIYNIIRIIFLWKKFRLFPLTI
                     QSLYTLLLAGGCYGVCYFALKEIHGFGGMIIRSIVFIALYATGVISMKLSPDIQPVVQ
                     TIKKRLGIK"
BASE COUNT         1399 a         1020 c         1054 g         1553 t
ORIGIN      
        1 aacattccct tcaatctgcg tcagccataa gttagattct ttggaggtgg caacaggtat
       61 aagttctgtc aggtcttttg gtgcaatgat gatacctgct gcgtggatac ctgtatttct
      121 tacagaacct tccagtcttt cggcctcatg cagcaccttg ctgagtatgg tatcttcctg
      181 tttatagatt tcacggagtt tctttacgtt ttccnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnng atccattact ctggccacat cggcaatact tgacttggca gccatcgtac
      421 cgtaggtaat gatttgggct acctggttct taccatattt ttccactaca taatcaatta
      481 ccttttgcct tccttcatca tcaaagtcag tatcaatatc aggcattgat ttacggtctg
      541 gatttaagaa tctttcgaac agtaaattgt acttgatggn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaatcacc cacaatgagg aagtatccgg
      721 caaaacccat tgttttgatg gtaaacaatt caaagtctat tctttctttt atctcgtcag
      781 tgagttcgct gtatcgctgt ttggcgcctt cgtatgtaag atgtttcagg tattcccact
      841 ggttcagtgc ttcggggctg atgacgttct tgccaatgat ttcttctttg gtatgaacct
      901 ggaatctttt ttcaatgggg aaattaggca acaggatatc acggtgcagg ttcaatagct
      961 ctactttgtc aacaatttca ttagtgttgt caatagcttc aggaaggtca ttaaaagctt
     1021 ttgacatttc ttctgtagtc ttgaaataga actggtcgtt ggggaaagca aagcgtttgt
     1081 cttttacatg cacatcgtcg tctgagaatt cccgcagggc aggtgtggtt attttttcgc
     1141 cggtattgat acaaagtaaa atatcatgtg cgttgaaatc gtcctgttcc acataatgtg
     1201 aatcgttgct ggcaataatc ttaacgttgt atttttttgc cagtttaatg agtacttcat
     1261 tcaccctgtc ctgttcggcc ataccgtgac gctgtaactc tacataataa tcttcctgga
     1321 aaatattcaa ccaccatttg aattcgtttt cgccttcttc ttctcctttc ttcaatattg
     1381 cctgtggcac catggcgcca agacagcagg tggtagcaat taatcctttg tgataacgca
     1441 gtatcagttc cttgtcaata cggggatatt tactgtacat tccttcaata aacccaagcg
     1501 atgtcagctt aaccaggtta cgatagcctt cttcattctt tgcgagcaat atctggtgat
     1561 gccggggatc tttctggtct ttggtgaatg ctttctggtg ccggtcggcc gttatgtaaa
     1621 attcacagcc aacaattggt tttacgatgg gtttaccatc tgcccctttg tttttataag
     1681 cttcggctac aaattcaaat acaccaaaca tattgccatg gtcactgatg gccagggcag
     1741 gcatgccgtc tttgatcgct tttttataaa gattttgtat agaggctgca ccgtctagta
     1801 aagagaactg ggtatggaca tggagatgag agaatttcat aactcactaa ccttatttct
     1861 ttttaaaact ataaatactg attgaaccgc tgctgtaact gcacacccag gcttccagtt
     1921 tatcatcgtt ctcaaaaata tcaacaccca ccgggtggcc gccacaggac aaagatgcca
     1981 ctttctcgaa gttgtcgttg ttgattttgt acacatctac atagtcgctg gtataacagg
     2041 taacaaaaat gaatttgtgg tttttagata atacaatggt tctgggttgt gcatgggttt
     2101 tggcagtgaa aagctgctgg ccggtagctg catcaataca agcgatagtg cccaggctgt
     2161 tgtaagaaac aaacaaatgg ccgctggtgt ccattaccat atgccggggg ctgctccaaa
     2221 cattcataat gctttcactt ttccaggtag tattgtccac cacattcagg ctggagccac
     2281 ccataatagc gatataagac tttttattct tgtcgtctac ggcaatgcct ctcggaatag
     2341 ccgaaacagg aatagtgctg atcacttttg catagggcag tttggtttca tccatttcca
     2401 gtacacttgt gttacgggag tgccagttac ttaccaaaag atatttgctg tcagcagtcc
     2461 tggatattac tttgggcgtt ttgcctgttt caatcagcgg tacccgaaaa gaatcttttt
     2521 tggctgtgcc gggataaatg acagtaaccg gttttgtttt ttgctcagct gttgttacag
     2581 cattgttttt tgaataatca tctacccaaa tcgggacaat accatctgca ttgtgcagcg
     2641 atatccataa aattttatca tcgtgactaa aacaggcctc taccggtttt tcctgaaaag
     2701 aagcaatagg acggcttctg ttataatccc acccggtgcc acgggtaggt gtaaatttaa
     2761 actcacggct aatttttcgg ctggcctggt caaattcata tacactcatc ccttccaggt
     2821 tcatggcata gagttttgaa cctgaggaat taaagatcac cgatttggtg ccgcctgcag
     2881 ccgtcaatac ttcttttttc tcgtactcaa tacttgaata gggtttaagc ttaggggctg
     2941 gtggaatagt atctgcattt acatgtttgc taaacagtgt ttcaatgtca ggtttctttg
     3001 aatttccgga tgtttggcag ccgatagcca gaaaataaag agacaacagg gaaaataagg
     3061 tttgcatggt ggtttaatct tagcttcaat aataggcaaa taattgtttc cggccatgcc
     3121 tgattttcat caatatccac agcctgtgag taagctaagt cgtttgaaaa caggaattta
     3181 ggcttgtggc aattttacta caggtttttc gtttaaggaa tgtggttttt tttggctttt
     3241 aacttatatt tttgcgcacc tgcctaaggg taggtaatca gaagtaaatc aaacatattt
     3301 tgaatcatcc taaacgaaag acgtaataat ggttaggcag attttaccag cagtagcagc
     3361 agtcatattt ttaagcagtt gttcaacctt caagtcgctg aattttacca gcaataagca
     3421 ggttactact actgtgcaac aactggatgc accccgaaca aaatttattg atgaaattgc
     3481 tgtaacgcct gttgccgctc agcagcagtt gaaactggag aaagcagaac ctaagtcagg
     3541 tcctgcggcc agcatttctg accgtttagt caatataccc gagccaatca aacaagaagt
     3601 tgcgcagccg gaaaaatcca ttgttacccg tgaagcagaa attgaaagtg catccgaagt
     3661 gcagttcaaa tatgcgatcc taatgaacac cgaagtggag tcattgccca gcaaacaatt
     3721 actggaaacg nnnnnnnnnn nnnnnnnggt gttcgttatc gtacaggtgg caatactaaa
     3781 agcggggttg attgttcagg gtttacagta gcagtttact cagcagtttt tggtattatg
     3841 ctgccaaggg tttcaagaga gcaatatcgc atcagccgaa aaatatctac aacggaatta
     3901 caacaaggtg atttggtatt tttcaatacc aatggccgtg gtgtatctca tgttggtgtt
     3961 tacctgggta ataacaaatt tatccatgct tctgtttcaa aaggagtaat ggtgaacgac
     4021 ctgtttgagc cttattacct caaacgctat attggagccg gaaggataga tgataagcag
     4081 atggtggctt caggaaacta atcttttatt ttattcccaa tctttttttg atcgtttgta
     4141 ctaccggttg aatatcgggt gatagtttca tgctgataac tcctgtagcg tatagggcta
     4201 tgaatacgat acttcttata atcataccgc caaatccatg tatctccttt aaggcaaaat
     4261 agcaaacgcc ataacaacct cctgccagca acagggtgta taacgattga atggttaatg
     4321 ggaataacct gaattttttc catagaaaga tgatgcggat aatattataa atgctgatgg
     4381 aaataagtgc agctatggcc gggccaataa gtccatattg cttggtcaaa atataggtca
     4441 gcggcagcat tacactaagc aggatgacac cacttataag ttcaaaccgc cagtaggtgg
     4501 atgtaccgat tatctgtgag ttaaccccgg tgcccatatc aattattttt gttattccca
     4561 ataagaagaa aatccaggca gcatcaaggt aactgggttt tacaccaaaa gtaataatgc
     4621 catctgtaaa gttcagccag atgagcagga agatgccaca ggcaaaaatc aataggttaa
     4681 tggatgaccg ctgatatact ttttgcaaca gtgccatatt cttgtctttc catgccttgg
     4741 ataaatgcgg aatggaagcg gatattatcc ctctttgcgg ggcttgtatg atacttgtaa
     4801 caatggtagc caggccaaaa atgccggctt ttgcagatcc ttcctccagt aacgaagcaa
     4861 ttacgatcgt atcaaacacc tgtgaaatag taaagatgag tgtgccgcca tacacaaaac
     4921 tgcaaagtgc cagtatcttt ttaaaatacc ggcggctgac cttgcttact gtaaaggtga
     4981 agtgaatctt tttggtgata accaggtagg ccaaaaggct taaagcaata cttggataac
     5041 taaatgcaaa gatttttata aacagatcgt aatctttaat gatattacta ataaataata
     5101 caatcagtac agttgtaaat aatctccact gcacttccct gaaaaagtta gtcaatacag
     5161 atttgtgcag gctccatgca taggcttcca gcactgtata aattgtgagt ccaagtccga
     5221 gtggaaaaat ccaataatag taagcaacca gctgcggagt attggtaccg aattttttta
     5281 tgaccaggtg tttcagcgtc cagccggcaa tc
//
LOCUS       DAORLS010000220         5293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8504_length_5293_cov_1.927835, whole genome shotgun sequence.
ACCESSION   DAORLS010000220 DAORLS010000000
VERSION     DAORLS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8504_length_5293_cov_1.927835"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>981)
                     /locus_tag="PK977_14540"
     CDS             complement(<1..>981)
                     /locus_tag="PK977_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19390.1"
                     /translation="LNTRHYLPFERLGYVYMNTTRYAAADSFFLEADIRKRGYNFNPS
                     DLIHVDPIDESIVMSPHLPCDFDTNMVKKNDVLGNLYIGLLYVDYKAFPKAEKKFKEV
                     INIDPDNPLVFHFLGKLYYDQQRWQEADIVFNYALQFHLDKYALQRYCDSLAKLFPYY
                     LSVNDEKIHCVLRRVQYSWVPKMETRFLLGTAYERWNHVEEAEKQFRAIMKEEPTEIA
                     AYYKCWTLLEKNERYHDAEAVIKSIADKSVVRNELLPFYKRVTAKYPEWGEWFYKAGS
                     MVYDMAMEAPELYENDRKYIKDDTDEESYMNRPYMDQSVTGNKVPATGDHF"
     assembly_gap    982..996
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<997..2719)
                     /locus_tag="PK977_14545"
     CDS             complement(<997..2719)
                     /locus_tag="PK977_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF19391.1"
                     /translation="MANERKTYAVIVGISAYESDGIPKLNFAHRDAEEFEKFLLSKAG
                     GSVPSDNIRILLNTDATYTSIYNAWYWLLETANKDDLVYFYFSGHGDMENNTIDNLGF
                     LLAVNTPRTNYLHNAIRIEDINRFANTLSVTKGSRVILITDACHSGDLAGKNIRGNQL
                     TGDQLRTATGKEIRITSCGPNELSNEDEKWGGGRGVFSYYLVKGLEGLADFSNDRVIT
                     LEEVKSYLTQSFQADKILSQKENKQTPVVSGTLPTPLARVDKQRLDSLRLSVSPAPIV
                     EQTSRPKPMGTSPQGYFFSLVDKWNVEKQVNFHQLRLLPSYEIPYEFIRQVTETVRQK
                     ITYDSLQVNRLIKSLETNADALKRFNDKLIEVLSDRGQRMINLYLEGDEAELEKRRYY
                     SKADTDYDVFAAMLDVALKISKPRSGIFSYSAVATGSDAYLPECLELKYHYFSGVATR
                     LKMPLFKNTDSLLAVALREQQEAYKIEKNAAYINNELGILYKQKNNLAEAEKFFLSAT
                     QIAPNWAMPWANLAGLYAQMNKRESGFAAAAKARDLQPGFQGAYVNTGILYEKDNNLL
                     FAEEYYRK"
     gene            complement(2865..3350)
                     /locus_tag="PK977_14550"
     CDS             complement(2865..3350)
                     /locus_tag="PK977_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19392.1"
                     /translation="MAKKALTKDSEFIVGEEKILNYILAFLFLIIFVLGLVPTLKNGF
                     STDRILDYLLMMALLPSFMFFTKARSRRVYIRVNKTGIYQDEMLVTGWDKFLNAYIKE
                     KPLTLKWRDNFMLVIEFTKEDPTKGFRRFIKLTNTQNKSEEDIIAAIKFFWQLYQSGR
                     S"
     gene            complement(3415..4461)
                     /locus_tag="PK977_14555"
     CDS             complement(3415..4461)
                     /locus_tag="PK977_14555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRF19393.1"
                     /translation="MELGISMFGDNHFDKAGKPLPTGQRLQELIEEIKLMDEVGLDFF
                     GVGEHHRPDYAVSVPEIVLAAAASVTKQIKLGSAVNVLSSSDPVRLYQSYATIDLLTN
                     GRAELMAGRGSFIESFPVYGYRLEDYEELFEEKLELLVKINKENPITWKGKHRAALNK
                     QSILPRAYNDNLNIWVAVGGTPESVLRAGRLGLPVIFAIIGGNPAQFKPLFEYYKKVY
                     DHFEHDATKLQMGVHMHAFFGEDSQSTADYYYPIYSAQMDRVGSSRGWPPYQRQQFDA
                     GRGNKGHLVIGDVNEAIDKILELQELFGLTRFSAHMDVGGPSHASLMKSIELFGTKIA
                     PKVREALGGRSRES"
     gene            4693..>4953
                     /locus_tag="PK977_14560"
     CDS             4693..>4953
                     /locus_tag="PK977_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19394.1"
                     /translation="MKIFRFMFVFALLITATAVIGQKEQNISSMKEGTVLTAADIGFL
                     SMVSNAELDASRGPAVRGATINNVFYKAGQTLSKADAKAITLA"
     assembly_gap    4954..4971
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1468 a         1237 c         1025 g         1530 t
ORIGIN      
        1 aaagtggtca ccggtagcgg gaactttgtt tcctgttact gattgatcca tatacggacg
       61 gttcatgtaa gactcttcat ccgtatcatc cttaatatac ttgcggtcat tttcatataa
      121 ctccggcgct tccatggcca tatcataaac catgctgccg gctttgtaaa accattcacc
      181 ccattccggg tactttgcag ttactctttt ataaaaaggc aataattcat tccttacaac
      241 agatttatct gcaattgatt taatcacagc ttccgcatca tgataccgtt cgttcttttc
      301 aagaagggtc cagcatttat aataggccgc tatctcagtg ggttcctcct tcatgatagc
      361 ccgaaactgt ttttctgctt cttctacatg gttccatcgt tcataagcgg tacccagtaa
      421 aaatcttgtt tccatctttg gaacccagga atattgaacc ctgcgcaata cacaatgtat
      481 tttttcatca tttaccgaca ggtagtaggg aaagagtttt gccagagaat cacaataccg
      541 ctgcagtgca tacttatcca ggtgaaattg aagggcataa ttaaaaacga tatccgcctc
      601 ctgccatcgt tgctgatcat aataaagttt accaagaaaa tgaaaaacca gcgggttgtc
      661 cggatctata tttatcacct ccttgaattt tttttccgcc ttcggaaaag ccttgtagtc
      721 cacataaagc aatccgatat ataaatttcc cagcacatca ttctttttga ccatgttcgt
      781 gtcaaaatcg cagggtaagt gaggactcat gacgatgctt tcgtcaatcg ggtcaacatg
      841 aattaaatca gaaggattga aattatagcc ccgtttccgg atatcagctt ccaggaaaaa
      901 actatcagca gcagcatacc gggtggtgtt catatataca tagccaagtc tttcaaaagg
      961 caggtaatgc cgtgtgttta gnnnnnnnnn nnnnnntttt acgataatat tcttctgcaa
     1021 acaatagatt gttatctttt tcgtaaagga taccggtgtt tacataagca ccttgaaaac
     1081 cgggctgcaa atccctggct ttggcagcag cagcaaaacc gctttccctt ttattcatct
     1141 gtgcataaag accggccagg ttagcccagg gcatggccca gttcggtgca atttgtgtgg
     1201 cactcaggaa aaacttttcc gcttctgcta aattgttttt ttgcttatat aaaattccca
     1261 gttcgttatt tatgtaagct gcattttttt caatcttata agcctcctgc tgttccctta
     1321 aagccactgc cagcaggctg tctgtatttt taaataaagg cattttaagc ctggttgcca
     1381 caccggaaaa atagtgatac ttaagctcca ggcactcggg taaataagcg tcagaacctg
     1441 tggcaacagc gctgtaacta aaaatacctg aacgaggttt ggatatcttt aatgccacgt
     1501 ctaacatagc agcaaaaaca tcataatcag tatccgcttt actgtaatac cttctttttt
     1561 ccagttcggc ctcatctcct tccaggtaaa gattgatcat acgctgacct ctgtcggaaa
     1621 gcacttcaat caatttatca ttaaatcgct tcagggcatc agcattcgtt tcgagtgatt
     1681 taatcaaccg gtttacctgt aatgaatcat atgtaatttt ttggcggacg gtttccgtta
     1741 cctgccgtat gaattcataa ggaatttcat aagaaggcag gaggcgcagc tgatgaaaat
     1801 tcacctgctt ctccacattc cacttatcaa ccaggctgaa aaagtagcct tgcggagaag
     1861 tacccattgg tttaggcctg ctggtctgtt caactatcgg cgcaggagaa acggaaagcc
     1921 gcaaagaatc taagcgttgt ttatcaaccc tcgctaaggg tgttggtaat gtaccgctga
     1981 ccaccggcgt ttgtttattt tctttttggc ttaatatctt atctgcctga aaagattggg
     2041 taagatagct cttgacttct tccagtgtaa taaccctgtc attggaaaaa tctgctaaac
     2101 cttccaggcc cttcaccagg taataggaaa agacaccacg gcccccgccc cacttttcat
     2161 cttcattgga tagttcgtta gggccacagg aggtaatacg tatttcttta ccggtagctg
     2221 ttcttaactg atccccggtt aactggtttc ccctgatatt tttaccggcc aggtcgccgg
     2281 aatgacaggc atcggtaatc aatatcactc ttgatccttt tgtaaccgaa agggtattgg
     2341 caaaccggtt aatatcctct atacgtattg cattatgcag gtaattggta cggggggtat
     2401 taaccgccag caggaatccc aggttatcaa tcgtattgtt ttccatatcg ccatgaccgg
     2461 aaaaatagaa ataaacgaga tcgtctttat tggcagtttc aagcagccaa taccaggcat
     2521 tgtaaataga agtgtaagta gcatcagtat tcaataatat ccggatatta tctgacggaa
     2581 ctgaaccacc tgcctttgaa agaagaaatt tttcaaattc ttcagcgtcc ctgtgtgcaa
     2641 agttcagctt gggtatgcca tcactttcat acgcagatat gccaacaata acagcatacg
     2701 ttttcctttc gtttgccaaa ttactatttg actgtgccca aatgctgagg gggcagcaag
     2761 aaataaatag gattataagc agtagccggc gcataggtaa aataatatca atcagttaat
     2821 ttaccgatat tatttgattg agaagaaaaa acatattgaa acagctatga cctgcctgac
     2881 tggtacaatt gccagaaaaa ctttatagcc gcaataatat cttcctcaga tttattttgc
     2941 gtattggtga gtttaataaa gcggcgaaag cctttagtgg gatcctcttt tgtaaactca
     3001 atcaccagca taaaattatc ccgccatttt aacgtaaggg gtttttcttt tatataagca
     3061 tttagaaatt tatcccagcc ggtcaccagc atttcatcct ggtagatacc ggttttattc
     3121 acccttatat acactctcct gctccgggcc ttggtgaaaa acataaaaga tggcagcagg
     3181 gccatcatca ggaggtaatc cagaattctg tcagtgctga aaccattctt caacgttggc
     3241 acaagcccaa gaacgaatat gatgagaaaa agaaaggcca gtatatagtt cagaatcttc
     3301 tcctctccca ctataaactc gctgtctttt gtcaatgcct ttttcgccat gcagtaaaaa
     3361 tagctattca tcaacaatcc ccttgctcct tactcccgac tacggactac cgactcatga
     3421 ctctctgctc ctgcctccca aagcctctct caccttcggt gcaatcttgg ttccaaatag
     3481 ttctattgat ttcatcagcg atgcatgcga agggcctcct acatccatat gtgctgaaaa
     3541 acgggttagg ccaaacagtt cctgcagctc cagtatttta tcaatcgctt cattgacatc
     3601 cccgatgacc aaatgcccct tattgccacg gcctgcatca aactgctgac gctggtaagg
     3661 cggccagcca cgactgctgc ctaccctgtc catctgtgcg gaatagatag gataataata
     3721 atcagcggtt gactggctgt cctcaccaaa aaaggcatgc atatgcactc ccatctgcag
     3781 tttggtggca tcatgttcaa aatgatcgta cactttttta tagtattcaa acaggggttt
     3841 gaactgtgcc gggttgccac caatgattgc aaaaattacc ggcaatccca ggcgaccggc
     3901 ccgcagcaca ctttccggtg tgccacccac agccacccag atattcagat tatcattata
     3961 ggcacggggt aatatggact gcttgttcag tgctgcccgg tgttttccct tccaggtaat
     4021 agggttctcc ttattgatct ttaccagcag ttccagtttt tcttcaaaca actcctcata
     4081 atcttccagc cggtaaccat aaaccgggaa cgattctatg aaactaccgc ggcccgccat
     4141 cagttccgcc cggccattgg tgagtaagtc tatggtggca taactctggt acagccgcac
     4201 agggtcagat gaactgagca cattcacggc gctgcccagc ttgatttgct tcgttaccga
     4261 agctgcagct gctaatacta tctccggaac agaaacagca taatctggcc ggtggtgttc
     4321 ccccacacca aagaaatcca ggcccacttc atccatcagt ttgatttctt caatcaattc
     4381 ctgcagccgt tggccggtgg gcaagggttt acctgcttta tcaaaatgat tatcgccaaa
     4441 catactgatg ccaagttcca tgtgatgctt ttatcgcaaa tatagttacc agccagcgga
     4501 gaactgaact acaatagacc gcaggtatcc cttgccgctt cggacatata caaaatttaa
     4561 tttcaacacc tgatataagt cacccttttg ggtattattt cgacgttaca tttaacagca
     4621 aatgagaaaa gtctccaagg ggactgaagt atctgagcca aaacaccatt tcttaaccaa
     4681 aagcgaaaga ctatgaaaat attcaggttc atgtttgttt ttgcgttact gataacagct
     4741 actgctgtca tcggtcaaaa agaacaaaac atttcatcaa tgaaagaagg cacagtactc
     4801 actgctgccg atattggttt tttaagcatg gtatccaatg ccgaactgga tgcaagccgc
     4861 ggacctgctg taagaggagc cacaattaac aatgtattct acaaagcagg tcagacactt
     4921 tctaaagccg atgctaaagc aattacctta gccnnnnnnn nnnnnnnnnn naccgaattc
     4981 cagaaaaatt acaaagctcc ggatggcagc cgcggtgccg gctggtgcta ctattggtat
     5041 tactattgcg actattgggg atattgctat tggtacaaat attggtacta ttgctaaacc
     5101 cattaagtga gaaaagccgg tttacgttac cggctttttg ctatcttttt gttttattcg
     5161 ctttcttctt tgttaatacc cagtataatc ctcttacatt ggtttgccaa tccggcagaa
     5221 cagccgtaat aacaatctct tccagcagtt ctagtagtgt aatacccgct gccgcataaa
     5281 acaagacaag att
//
LOCUS       DAORLS010000221         5276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8538_length_5276_cov_1.538594, whole genome shotgun sequence.
ACCESSION   DAORLS010000221 DAORLS010000000
VERSION     DAORLS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8538_length_5276_cov_1.538594"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>518
                     /locus_tag="PK977_14565"
     CDS             33..>518
                     /locus_tag="PK977_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRF19395.1"
                     /translation="MANQKTAVWGKEKVFKTGDNGYRAYRLLPANGIFETTKDAVTLC
                     IGLIGIMALFMGFMSIAEKAGGIRFLSRIIGPFFSKIFPEVPKNHPSMGHMVMNFSAN
                     LMGLDNAATPFGIKAMESLQEINPDKTKASNAQLMFLCLHASGLTLIPTIIIADRVAL
                     KS"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <529..1258
                     /locus_tag="PK977_14570"
     CDS             <529..1258
                     /locus_tag="PK977_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HRF19396.1"
                     /translation="HPTDIFIPCMIATFVATIAAMTIVAIRQRINLLQPVILFWVLGI
                     STVIAALIYYIKSLDAQGVQLFSTSLSNGIILLIFLLIVLGGIYKKINIFDAFIDGAK
                     GGFETAVRIIPYLVGLLVAISLLRNSGAFEFILDGIKHLFALLNGDARIVDALPTALL
                     KPLSGSGARAIMIDTLNAHGPDSFAGNLASVFRGSSDTTFYIIAVYFGAVGIKNTRYA
                     VTTMLLADLAGIITAIAMAYIFFT"
     gene            complement(1333..>1716)
                     /locus_tag="PK977_14575"
     CDS             complement(1333..>1716)
                     /locus_tag="PK977_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19397.1"
                     /translation="TNIEVPTDTTARIRFAHFPYSPTEIPAVDIFSVKRNANIFTNVS
                     LTEVTGFIPIASGVTDTFYIRPAGTGTNLQNFNPSPAPGAWSNIQAVLTPTIKRSYTL
                     IFRGGYRATTTTNATVRTLSVFVNR"
     assembly_gap    1717..1745
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1746..>2052)
                     /locus_tag="PK977_14580"
     CDS             complement(<1746..>2052)
                     /locus_tag="PK977_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19398.1"
                     /translation="KSFDEKFKEQDDFSNSSIMQVYVATVNASRNFIWVDANRINGAS
                     LTTGSVFPTTGFGFNVGGGLRNFLVRDTLSTSTQVPYSFAENLQAGKTYTLFMYDTIT
                     "
     assembly_gap    2053..2117
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2154..3800)
                     /locus_tag="PK977_14585"
     CDS             complement(2154..3800)
                     /locus_tag="PK977_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF19399.1"
                     /translation="MKLRNKLIAIPLTLITVSGLFSCSKKIDEAYTNPNASTRVPVET
                     LLPGIIGNFVGSSSAQGSAYGIANDGLYTGRYAQFWATNTTANQYDQMGGATGASDIL
                     GSIWAMHYYGHGANLSKMIEWATEDKKWDYVGVGYAIRAWGWLTLTNMYGEAILRQAF
                     DPNRLVFDYDSQEDIYAETRRLCHVAIEYLSKTGDGVSQANLALGDAYFYNGDVNKWK
                     KFCYSVLARSFNHLTNKATYNPDSVIYYANLGILDNADNASAKFAATGLSGTYNFFGP
                     FRGNVGALRQSRFIANLMTGLNPTFLSVNDPRAPYIIRENTNGTYRGVRPNKGTDGLA
                     TNDQPQNFWGGAFSSTAAPGTDATCRYIFRNGSPFPIITAAEVHFMKAEAYLRKGPAF
                     AAQARQSYIDGINASFDMLTGTPDYHNSVPGAMQITPGVKAAYLANPTVVPAVGNLTR
                     SHIMLQKYIAMYGYGVVETWVDLRRYHYTDLDPVTGQQVYADFAPPTGTDLFTNNAGK
                     WVYRARPRYNSEYLYNIAALSSVGGLALDYHTKEQWFSQP"
     gene            complement(3823..>4096)
                     /locus_tag="PK977_14590"
     CDS             complement(3823..>4096)
                     /locus_tag="PK977_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19400.1"
                     /translation="EEEFIEKDVNWFRLRDVTINYTFSKNFIRSFKSLSLFVTANDLI
                     LLTNYSGADPAVNGNTAGSSGVGGFGFDYGTLPSPVQVNFGIKASF"
     assembly_gap    4097..4161
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4162..>4411)
                     /locus_tag="PK977_14595"
     CDS             complement(<4162..>4411)
                     /locus_tag="PK977_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19401.1"
                     /translation="GDGVFTVKGDRNPDFTLGWNNTVRWKNWRFSMLWDLKIGGDIFN
                     GTNMFLTRTGKSKRTEDRYVPRVIQGVLNDGLQNSATPT"
     assembly_gap    4412..4550
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4551..>4805)
                     /locus_tag="PK977_14600"
     CDS             complement(<4551..>4805)
                     /locus_tag="PK977_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF19402.1"
                     /translation="LNIDFTYYNTLNKGQIIQNMRLSYGTGYVLNTQNAASTRNKGIE
                     IAMDYDLLKKSDLTWNMRFNFNKMYNKVVDMPRNVAEYYI"
     assembly_gap    4806..4921
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4922..5186)
                     /locus_tag="PK977_14605"
     CDS             complement(<4922..5186)
                     /locus_tag="PK977_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19403.1"
                     /translation="MAYFGEFAVSFRNYIFLSYTHRFEEASTVPKQNRKYNYPGGSLS
                     IIMSDMIPSLKKNNLVSYWKLRTSLAGTARLNTPYSTQSVFVDN"
BASE COUNT         1312 a         1063 c         1044 g         1433 t
ORIGIN      
        1 cagcatcatt atcgctgaca gttcatacgc agctggcaaa ccagaaaacg gcagtatggg
       61 gtaaggaaaa ggtatttaaa acaggggata acggttacag ggcttacagg cttttaccag
      121 ccaacggtat atttgagacc acaaaggatg ccgttacgct atgcatcggt cttattggca
      181 tcatggcatt gttcatgggc tttatgagta ttgctgaaaa agccggtggc atccggttcc
      241 tttcccgcat tatcgggcca ttcttttcaa aaatatttcc cgaagtaccc aaaaaccacc
      301 cttcaatggg gcatatggtc atgaattttt cggctaacct gatggggctg gacaatgctg
      361 ccacaccttt tggcataaag gccatggaaa gtctgcaaga gatcaacccg gataaaacaa
      421 aggcatccaa tgcacagctc atgtttctct gcctgcatgc atccggcctt acgctgatac
      481 ctacaattat aattgctgac agggtagccc tgaaatcann nnnnnnnncc atccgactga
      541 tatttttatc ccctgtatga tcgccacttt tgtagccact attgcagcta tgacgatcgt
      601 tgctattcgt cagcgcatta acctgctgca accagtgatc cttttttggg tactgggtat
      661 cagtaccgtt attgcagcgc tgatctatta catcaaatcg ctggatgcgc agggcgttca
      721 gctgttctca accagtctga gtaatggaat tattttactc atctttttat tgatcgtttt
      781 gggaggaatt tataagaaga taaacatatt tgatgcgttt attgatgggg ccaaaggagg
      841 gttcgaaact gccgtacgga ttattcctta tctcgttggc ttgctggtag ctatcagttt
      901 gctgcgcaac agcggtgctt tcgaatttat cctggacgga attaagcacc tctttgccct
      961 gctaaatggc gatgcccgta tcgtagatgc attacccacg gccttattaa aaccactgag
     1021 cggcagcggg gcaagggcta ttatgattga tacgctgaat gcacatgggc ctgattcttt
     1081 cgcaggaaac cttgcatcgg tattcagggg ttcgtcagat actacttttt atattattgc
     1141 ggtttatttt ggtgctgtgg gcataaaaaa tacccgctat gcagttacca ccatgttact
     1201 ggccgacctg gccggcatca ttacggctat tgccatggct tatatctttt tcacttaaac
     1261 ggtgtatttg tgtaaaataa agaagccgtt caatttgaac ggcttctttc tttgatttat
     1321 tctgtacact tattatctgt tcacaaatac tgataaagtt cttacagtag cattggtggt
     1381 tgtagttgcc ctgtaacctc ccctgaaaat tagagtatag ctccttttaa tcgttggtgt
     1441 caatactgcc tggatatttg accaggctcc gggagcgggg cttggattaa agttttgcag
     1501 atttgttcct gttccggcag gacggatgta gaatgtatct gtcacacctg aagcaatcgg
     1561 aatgaacccg gtcacctcgg ttaaagaaac attagtaaat atgttcgcat ttcttttgac
     1621 agaaaaaata tccactgctg gaatttcggt tgggctatac gggaaatgtg caaaccgtat
     1681 ccttgcggtt gtgtcagttg gcacttctat attggtnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnngtaat ggtgtcatac atgaaaaggg tatatgtttt accagcctgc aggttctccg
     1801 caaaagaata cggtacctgg gtgcttgttg ataaagtatc ccttaccagg aaattacgca
     1861 gacctccgcc aacattgaat ccaaaacctg ttgtagggaa aacactacct gttgtaagcg
     1921 aagcaccgtt aatacgattg gcgtccaccc atataaagtt tcggctggca ttcactgtag
     1981 caacatacac ctgcattatg ctggagttgg aaaaatcatc ctgttccttg aatttttcat
     2041 caaatgattt ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnntaa atttcatgat cttagtttta tgagttaatg actttagggt
     2161 tgagaaaacc actgctcttt ggtatgataa tctaatgcaa ggccacctac actggataag
     2221 gctgctatat tgtacaggta ttccgagtta taccttggcc ttgccctgta cacccattta
     2281 cctgcattat ttgtaaacaa atccgttccg gtaggaggag cgaaatcagc atatacctgc
     2341 tggccggtta cagggtcaag atctgtatag tggtatcttc tcaagtccac ccaggtttct
     2401 acaacaccgt atccatacat ggcaatatac ttctgcaaca tgatgtgaga ccttgtcagg
     2461 ttgcccactg ccggtaccac ggtgggattg gcaaggtaag cagcttttac accaggggtg
     2521 atttgcatcg caccgggaac tgagttatga taatcaggag tgccggttag catatcaaag
     2581 ctggcgttga taccatcaat ataagattgt cttgcctgtg ctgcaaaggc ggggcctttt
     2641 ctcaggtagg cttctgcttt catgaaatgt acttcagcgg cggtaatgat cgggaagggt
     2701 gatccattac ggaaaatata gcggcaggtt gcatctgtac ccggcgctgc agtagaacta
     2761 aaagctcctc cccagaaatt ttggggttgg tcatttgtag ccaacccatc cgttccctta
     2821 ttaggtctta cacctctgta agtaccgttt gtgttctcac ggattatgta aggtgccctg
     2881 ggatcattta cagaaaggaa tgttggattt aaacccgtca tcaggttagc tataaaacga
     2941 gactgacgaa gggcgcctac attacctctg aaaggaccga agaaattata ggtgcctgag
     3001 aggcctgtag cagcaaattt tgctgaagca ttatcagcat tatccaggat tccaagattg
     3061 gcataataaa taacggaatc aggattgtaa gtggctttgt ttgtcaggtg attgaatgac
     3121 ctcgctaaaa cagagtaaca aaacttcttc cacttgttta catcgccatt ataaaaataa
     3181 gcatcaccaa gggcaagatt tgcctggctt acgccgtcac cagttttact caggtattca
     3241 attgccacat ggcaaagacg cctggtttcc gcatagatat cttcctggct gtcgtaatca
     3301 aacaccagcc tgttgggatc aaaagcctgg cgaagaattg cttctccgta catattggtc
     3361 agtgttaacc agccccaggc acggatggca tagcccacac ccacataatc ccacttctta
     3421 tcttctgttg cccattcaat cattttggac aaattggcgc catggccata ataatgcata
     3481 gcccatattg aaccaaggat atcactggcg ccagttgcac cacccatttg atcatactgg
     3541 ttggcagtag tattcgttgc ccaaaactga gcatagcggc cagtataaag gccgtcatta
     3601 gctataccgt aggcactgcc ctgtgcagag gaacttccaa caaagtttcc gataatgccg
     3661 ggtaacaatg tttcaacagg cactcttgtt gaagcattag ggttagtata tgcttcatca
     3721 atttttttgc tgcacgaaaa caacccggaa accgtaatca gggtcagcgg aattgctatt
     3781 aatttatttc taagtttcat atttctaatt ttttaaaatt tatcagaagg atgctttgat
     3841 accgaaatta acctgtacag gagaaggcag ggtgccataa tcgaaaccga aaccacccac
     3901 accagaacta cctgcggtat tgccatttac agcagggtca gcaccgctat aattggtcag
     3961 caggatcaaa tcattcgctg ttacaaataa gctgagtgat ttgaatgatc tgataaaatt
     4021 cttagaaaat gtataattga tagtaacatc tctcagcctg aaccagttta catctttttc
     4081 aatgaactct tcctcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn ngtgttgggg tagcgctgtt ttgtaaacca tcattcagca
     4201 caccctggat aacacggggt acatacctgt cttccgtacg tttgctcttg cctgtacggg
     4261 tcaggaacat gtttgtaccg ttaaagatat cgccacctat tttcagatcc caaagcatag
     4321 agaacctcca gtttttccag cgtacggtgt tgttccaacc taaggtgaag tctgggtttc
     4381 tgtcaccttt aacagtgaac acaccatcac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atatataata
     4561 ttcagctacg ttgcggggca tgtccaccac cttattatac atcttgttga agttgaaacg
     4621 catgttccag gtcaggtctg atttcttcaa cagatcataa tccatggcaa tctctatacc
     4681 cttgttacga gtgctggctg cattttgagt gtttaataca taaccggttc catagcttaa
     4741 acgcatattc tgaatgatct gtcctttgtt aagtgtatta taataagtaa agtcaatatt
     4801 caaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 naattatcaa cgaagacaga ctgtgtggaa taaggcgtgt tcaaacgggc agtacctgcc
     4981 agtgatgtac gcaacttcca atagctgaca agattgtttt tcttaagcga tggaatcata
     5041 tcactcatga tgatactcaa gctaccacca ggatagttat atttcctgtt ttgtttagga
     5101 acagtagaag cttcttcgaa acggtgcgta tagctaagga agatgtagtt cctgaagctt
     5161 accgcaaatt ctccgaaata agccatttgc cttgatatct gcttattata ttctccaaac
     5221 ttatttctca gcaaacgtaa tcttgtattt ggccttgtaa cggtactatc catata
//
LOCUS       DAORLS010000222         5239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8623_length_5239_cov_1.960069, whole genome shotgun sequence.
ACCESSION   DAORLS010000222 DAORLS010000000
VERSION     DAORLS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8623_length_5239_cov_1.960069"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PK977_14610"
     CDS             complement(<1..464)
                     /locus_tag="PK977_14610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRF19404.1"
                     /translation="MKKTVLLLSLFFTMKLYAQQFISTGMIEFEVRTNNHKTFGDGIW
                     AEMFKDKLPQFSTKYYSFTFSNNKALYKYERQDEKNKIPFGGGNEEENIWFSDYAAGT
                     FTDQKFVFDDTYLLSDSLMKIDWKLSPNETREIAGFNCRKATGKIFDSVYVFA"
     gene            complement(475..>2762)
                     /locus_tag="PK977_14615"
     CDS             complement(475..>2762)
                     /locus_tag="PK977_14615"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF19405.1"
                     /translation="ITAMGERVTKVLVDGEEFFGSDPGIATKNLRADAVKEVEVFDKK
                     SDQAEFTGIDDGIKDKTINLKMKQKKGYFGKIEAGGGLKDKYNNAAMINAFKDKRKMA
                     AYGIMSNTGQTNLDWQDAQNYGGGADNMTSGITEDGGMWVSVTNDGDDNYWGGRNGIP
                     QNWSGGLHYSNKFGKDDKQSFNSGYKFNKVNAPMENSTFSRTFLPDSSWFSNSISRNF
                     NSSVKHAFNLTMDFNIDSANSIKWTTRANKRDTRNTADFYSEALSESLDSINNSSRIS
                     NNKTENNSLTSSLLWKHKFRKLSRTISINTDFSWNKTNSDGLLYALNRYYDKGLLSFI
                     DTTDQQNLRNSEGKSISTKIAYTEPLAKDFYLELSYSLAYNHNNNDRITNRKSSNGKY
                     EDVIDSLSNAFVFDRLVNTPGLNFRVNKKKYNYSFGASVGFSNFIQKNLTEGTRTNYH
                     FTNFFPRVSYMYKFKPSQNFRFNYNGSTNAPTLEQLQPIRVNTDPLNIYIGNPGLKQS
                     FQHSFSTGYSSYNILKEKNIWTTLSFNFTQNAFVQSSSVDSVGKRTFQTVNTNGVYNL
                     SLYTDYGFKITKSKLRVGFGPTANAYRNIEYINNVKNITTTTNYGIRFNLSKYVENKF
                     DFYISPSFTLNKSKASVNAAANASFWEIEGWANARVILPKKFEISTNANTQIRQKDPR
                     FTQNNNFTTWNASLTKRFFKENSFEMSLGLYDILNQNRGYQRNFSSFSFTENYYTTLR
                     RFWLLTLTWNISKNGKPATGF"
     assembly_gap    2763..2915
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2916..3237)
                     /locus_tag="PK977_14620"
     CDS             complement(<2916..3237)
                     /locus_tag="PK977_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF19406.1"
                     /translation="MKKALLYLTLSLLSVIAYNQSSIKGTVTDSVEKKNPENAVISLM
                     RQTDSVLVKYTRADKGGHFLLTNLKEGKYILMITHPFMGDYFDQVDVKSGLNEIGKVY
                     VTPKS"
     gene            3353..>5239
                     /gene="glgB"
                     /locus_tag="PK977_14625"
     CDS             3353..>5239
                     /gene="glgB"
                     /locus_tag="PK977_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRF19407.1"
                     /translation="MTIKKNKSPKTKVQASVNKQVSRKYEESNFIDTSKPVWNYSLLS
                     DEDVANYQAGTHYQLYKQFGSHSIQVNDIWGMYFCVWAPNATSVSVKGHFNNWKDHEY
                     ELNPRWDKSGIWEGFIPHFKLGEAYKYHIVGYAKRKLDKGDPFANFWEKRPHTASITW
                     DMFYEWKDEEWMKNRKKHNSLNAPWSVYEVHLASWQRPDKNDEESYNTYDELRERLVP
                     YVKETGFTHVEFMPVMEHPFDGSWGYQCTGFFAASSRFGDPQGLMRLIDAFHQEGIGV
                     ILDWVPSHFPYDAHGLFMFDGTHTYEYADMRKGFHPDWNSYIFNYKRGEVKSFLISSA
                     RYWFDLFHIDGIRVDAVSSMLKLNYSREHGQWEPNEFGGDGNLEAIAFIKDLNETIFR
                     DFPDVQTIAEEATDWPGVSKPTWQDGLGFGMKWMMGWMHDTLDYFKIDPVFRQFHQNK
                     FSFSMMYYYDENFMLPLSHDEVVHGKSPMIYKVPGDEWQKFANLRLLYTYMWTHPGAK
                     LLFMGNEFGQTKEWNYKSELQWELLQYDCHRLLKQCVADLNELLKKEPAMHVNQFNID
                     GFEWVDLNHRAESVIAYKRKGKKKGDDLLVILNMTPVVRHNWQIEVSGKPYKAEIFNS
                     DQTIY"
BASE COUNT         1420 a         1018 c         1098 g         1550 t
ORIGIN      
        1 gcgaatacat acacactgtc aaaaatctta ccggtagctt tgcggcagtt gaaaccggca
       61 atttccctcg tttcgttggg agatagtttc cagtcaattt tcatcagtga atcacttaac
      121 aggtaagtat catcaaacac aaatttctgg tcggtgaaag tacctgccgc ataatcactg
      181 aaccatatat tttcctcctc attaccacct ccaaatggta ttttgttttt ctcatcctgt
      241 ctctcgtatt tataaagggc cttattatta ctaaaggtga aagaataata cttagtcgaa
      301 aactgcggta gtttatcctt gaacatttcg gcccatatac cgtcgccaaa tgttttgtgg
      361 ttattggttc ttacttcaaa ttcaatcatc cccgtactga tgaactgctg tgcatacagc
      421 ttcatagtaa agaacaacga tagtagtaat actgtcttct tcatacgata aattttaaaa
      481 tcctgttgct ggtttaccgt ttttagaaat attccaggtc aatgtcagca gccaaaacct
      541 tcgtaaggtg gtataatagt tttcggtaaa actgaaactg ctgaagtttc gctggtaacc
      601 cctgttctgg ttcaatatat catacagacc cagcgacatt tcaaatgaat tctctttgaa
      661 gaaccgcttc gtcaggctcg cattccaggt agtaaaattg ttattctgtg taaaccgcgg
      721 atctttttgc cgtatctgcg tattggcatt ggtactgatt tcaaatttct ttggcaatat
      781 tacccgtgca ttggcccaac cttcaatttc ccagaaactg gcattggctg ctgcattcac
      841 actggctttt gatttgttaa gagtaaagga aggactgata taaaaatcaa atttgttttc
      901 tacatacttg ctaaggttga acctgatacc atagttagta gttgtagtga tattttttac
      961 attattgatg tattctatat ttcgataggc attagccgta ggaccgaaac ctacacggag
     1021 tttgctcttg gtgattttga aaccgtaatc agtatataaa cttaaattat agactccgtt
     1081 ggtattcact gtctgaaagg ttcttttacc cacactgtca acagaactgg attgtacaaa
     1141 agcattctgc gtaaaattga aactcaatgt cgtccaaata tttttttcct ttagaatatt
     1201 gtatgaactg tagccggtgc tgaaactgtg ctgaaaagat tgcttcaggc cggggttgcc
     1261 tatataaata ttcaatgggt ccgtattaac ccggatgggt tgcaattgtt caagcgttgg
     1321 cgcattagtg gagccgttat aattgaagcg gaaattctgg cttggcttga acttatacat
     1381 ataagatact ctcgggaaga aattggtaaa atgatagttg gttctggttc cttctgtcag
     1441 gtttttttga atgaaattgc tgaagcctac cgatgcacca aatgaataat tgtatttttt
     1501 cttattcacc ctgaagttga gccccggtgt attcaccagc ctgtcaaaaa caaaagcatt
     1561 gctcaggctg tctatcacat cttcatattt accattactg ctcttgcggt tggtaatacg
     1621 gtcattgtta ttatggttat aggccagtga ataactcagc tccaggtaaa aatctttggc
     1681 cagcggttcg gtataagcaa ttttggtgct gatactcttt ccttcactgt tacgcaggtt
     1741 ctgctggtcg gtagtgtcta taaaactcag cagaccttta tcgtagtaac ggttgagggc
     1801 atatagtagt ccgtcactgt tggtcttgtt ccaggaaaaa tcagtattaa ttgaaatcgt
     1861 tcttgagagc tttctgaatt tatgcttcca gagcaggctg gaggtaagac tattgttttc
     1921 tgttttatta ttactgatcc tgctgctgtt attgatagag tctaaacttt ctgataaagc
     1981 ttccgaatag aaatctgccg tgtttcgggt atctcttttg ttggcacggg ttgtccattt
     2041 aatagagttg gcagaatcga tattgaaatc catagtcagg ttgaacgcat gcttcacaga
     2101 tgaattaaag ttccggctga tactattgga aaaccagctg ctgtcaggta agaatgtccg
     2161 ggagaaagta gaattttcca tcggggcatt taccttattg aatttatagc cggagttgaa
     2221 actctgttta tcatcttttc caaacttgtt gctatagtga agtccgccac tccagttttg
     2281 cggaatacca ttgcggccgc cccagtagtt gtcatcccca tcattggtaa cactcaccca
     2341 cataccgcca tcttcggtaa tacctgaagt catattatca gcgcctccgc cgtaattctg
     2401 ggcatcctgc cagtcaagat tggtttgacc ggtattgctc atgatgccgt aagccgccat
     2461 ttttcgttta tccttgaacg cattgatcat ggcagcatta ttgtacttat ccttgagtcc
     2521 gccacccgct tcaatcttgc cgaagtaacc tttcttctgc ttcatcttca ggttgatggt
     2581 tttgtcctta atgccatcat cgataccggt aaattcagcc tggtcacttt tcttatcaaa
     2641 aacttctact tccttcaccg catcagcccg caggttcttg gtagcaatac cgggatcact
     2701 accgaagaat tcctcaccgt ctaccagtac cttggttacc cgttcaccca tggccgttat
     2761 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgctt ttaggcgtta catagacttt
     2941 gcctatttcg ttcagccctg attttacatc cacctgatca aaataatcac ccatgaaagg
     3001 atgggtgatc attaaaatgt atttgccttc tttgaggtta gtcagtaaaa aatgacctcc
     3061 tttatctgcc cgggtatatt tcaccagtac agaatctgtt tgacgcatca gggagatgac
     3121 ggcattttcc ggattctttt tttctactga atcggtaact gtacctttaa tagaggactg
     3181 gttataggca ataacggaaa ggagtgataa ggttaaataa agcagggctt ttttcatagc
     3241 cggtttatgg ttttgaagtt ggttggctgg ccggcaggcg cagcatctgc gaagctattt
     3301 ttacttaccc atatatgccg aaccgcttat ataattttac gttaaaggaa atatgacaat
     3361 aaaaaagaat aaatcgccaa aaacgaaagt gcaggcgtct gtaaataaac aggtaagcag
     3421 gaaatacgaa gagtccaatt ttattgatac atcaaaacca gtatggaatt attctttatt
     3481 atccgatgaa gacgtagcca attaccaggc cggcactcat taccagctat ataaacagtt
     3541 tggatctcat tccatacaag taaatgatat atggggtatg tacttctgtg tgtgggcgcc
     3601 taatgctaca tctgtttcag taaaaggtca tttcaataac tggaaagatc atgaatatga
     3661 actgaatccc cgctgggata aaagtggtat atgggaaggg ttcattccgc atttcaaact
     3721 gggagaagct tacaaatacc atattgtggg gtatgccaaa cgaaaattgg ataaaggtga
     3781 tcctttcgcc aacttctggg aaaaaagacc gcatactgcc agtataacct gggacatgtt
     3841 ttatgagtgg aaggatgaag aatggatgaa gaacaggaag aaacacaatt cgctgaatgc
     3901 gccctggagt gtatatgaag tgcacctggc cagctggcaa agaccggata aaaatgatga
     3961 agagagttac aatacttatg atgagctgag ggaacgactg gtgccatatg taaaagaaac
     4021 cggctttact catgttgagt tcatgccggt gatggaacat ccctttgacg ggagttgggg
     4081 ttatcagtgc accggttttt ttgctgcttc ctcaaggttt ggcgacccgc agggactgat
     4141 gcgtttgatc gatgcatttc accaggaagg tatcggggtc atactcgact gggtgccttc
     4201 gcattttcca tacgatgcac atggcctgtt catgttcgac ggcacacata cctatgagta
     4261 tgccgatatg cgaaaagggt ttcacccgga ctggaacagc tatattttca attacaaaag
     4321 gggagaggtc aagtcatttt tgatcagcag tgcccggtat tggtttgatc tttttcacat
     4381 cgatggaata agggttgatg ccgtaagctc catgctaaaa ctgaattatt ccagggaaca
     4441 tggacaatgg gaacccaatg aatttggcgg agacggtaac ctggaagcca ttgcttttat
     4501 taaagacctg aatgaaacaa tcttccgtga ttttcccgat gtacaaacaa ttgcggagga
     4561 agctaccgac tggcctggtg tatcaaaacc tacctggcag gatggactgg gctttggtat
     4621 gaaatggatg atgggctgga tgcatgacac actggattac ttcaaaatag acccggtatt
     4681 ccgccagttt catcaaaata agttctcctt cagcatgatg tattattatg atgaaaattt
     4741 tatgctgccg ctcagtcatg atgaagtagt gcatggcaaa agcccgatga tctataaagt
     4801 gccgggtgat gaatggcaga aatttgccaa cctgcgtttg ctctatactt atatgtggac
     4861 acatcccggc gccaaacttc ttttcatggg taatgagttc gggcaaacga aagaatggaa
     4921 ttacaaatcc gaattgcaat gggagttgtt acaatacgat tgtcaccgct tactaaagca
     4981 atgtgtggcc gatctgaacg aacttttaaa gaaggaaccg gccatgcatg tcaaccagtt
     5041 taatatagat ggatttgaat gggtggacct gaaccaccgg gcagaatcgg ttattgcgta
     5101 taaacggaaa ggaaagaaaa aaggggatga cctgctggtc attttgaaca tgactcccgt
     5161 ggtgcgtcat aactggcaga tagaagtaag cggaaagcct tataaggctg aaattttcaa
     5221 cagtgaccaa acaatatat
//
LOCUS       DAORLS010000223         5219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8677_length_5219_cov_1.997095, whole genome shotgun sequence.
ACCESSION   DAORLS010000223 DAORLS010000000
VERSION     DAORLS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8677_length_5219_cov_1.997095"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK977_14630"
     CDS             <1..279
                     /locus_tag="PK977_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRF19408.1"
                     /translation="PDPSKRKEIKLEDIEIGVPEYVPPSQEELTHTGKITFFNTEKGF
                     GFIKDLVTQESVFVHVNNLSEPVKENDKVSFEVESGPRGLMAVNVKKV"
     gene            complement(292..>694)
                     /locus_tag="PK977_14635"
     CDS             complement(292..>694)
                     /locus_tag="PK977_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRF19409.1"
                     /translation="PGSDPGVRYIKMKGRKVYEYAMKYVPAAMKQCLDQSGVEIQQLK
                     KVFIHQANEKMDEGMIKRLYQLYGVNEPPNDIMPMSIHWLGNSSVATIPTLYDLVVKN
                     KVDEHALEPGDVILFASVGAGMNVNAVCYRY"
     assembly_gap    695..706
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<707..1371)
                     /locus_tag="PK977_14640"
     CDS             complement(<707..1371)
                     /locus_tag="PK977_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRF19410.1"
                     /translation="MRSIITGTGCYIPPVVMTNKDFAAHLFYTEKQEPIDTPPTVVVE
                     KFKQITGIDERRYTNDELNTSDIGSQAAAAAIKDSGVNPEEIDQLIVAHNFGNVFKNT
                     IQTDAVPSLASRIKHALGIKSPNCIAYDILFGCPGWVQGLIQADAYFKAGIAKKALIV
                     GTETLSRVIDMYDRDSMIFSDGAGAAVLEYKEVNEDGPGIIGAAVQSHTVEEVDYINM
                     GMS"
     gene            complement(1462..1707)
                     /locus_tag="PK977_14645"
     CDS             complement(1462..1707)
                     /locus_tag="PK977_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HRF19411.1"
                     /translation="MTSEQIEKYVESAKRKESAVSIHFKDRQAVTGVFIEGTDYSELK
                     SKNLWRIVIETKYSQWKMNKDINLSRIFNGVSFTRLS"
     gene            1985..>3250
                     /locus_tag="PK977_14650"
     CDS             1985..>3250
                     /locus_tag="PK977_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19412.1"
                     /translation="MKMLRLKGITFSFFISFSTLKAIAQPRPGDALLQINPGGHLSQI
                     SEAVTTADGKFIITTSTDKTICIWDVQQKKIIEQIRGPKSVFNQGKLYSLAISPDNRL
                     LAVGGFLAIGTETDGDLAGQIRIYDFAKRKQVLRFKAHSNVVTSLRFSADGNYLVSGA
                     NDSTIASWKVSGTIDKPSFSLVKKVVREDFYVEDIQVSGNAVFVTDRKGVNKYSLPSL
                     ELVSASSQYSSDMYCIAINRAANTIAMVSFDQLVISDTALKDKQVIELDSYSNRVAIS
                     PGGNYIITEGENSTVQLYSREASQFVKTVSADFKGGNTMLGMGFLSNSSFFVAGGRAN
                     IMQFYRIESRNSNIIKTDTVLAGSGQAFNAISVHEKKLALRSLEKYEGWFDYSLLPEA
                     GKLMSFQLSDSSKYPVKRNEKGSLSVSVNR"
     assembly_gap    3251..3270
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3271..5078
                     /locus_tag="PK977_14655"
     CDS             <3271..5078
                     /locus_tag="PK977_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF19413.1"
                     /translation="IYSGNKLTGTVKRDGASGYGHNAVTIIKSNWIISAGSAGFLTLY
                     DSLGTARASFVGHEGDVLDISESEDGNFLYSTGSDQTTRMWDLREVKGDLEFKSYDQL
                     DLTWKNFFKDYFPEYDFTRPGEMKKVYERLLSMGSKENADFLLIPQQLEPRLNIFIAK
                     NNEWVIWNNKGYFKASPNGASYIGWYVYKGEDQNAEFYTADKLFDNYYKPGIINELAL
                     SDESTVTILRKAGDAAKTSIAQQVSNMPVLKLAAPSVSEKAAQKNISLVIDVDNKDFI
                     SELLLFQNGKRITVSPDIFRGISLPSITIPVELVNGENTFIVSALNQSRVETTPLKFS
                     ISFGGTKASSSLYIMAVGVDKYKNSRYNLNYAKADARGIAAQLASSSSKIFKDIIVDS
                     LFDEIATTENIAERINRLKSQIKPEDVFLFYYAGHGIMSDPADGSKSDFYLVLHKVTQ
                     MQGNDEVLKTNGLSASRLRDLLLEVPAQKQLILFDACNSGGALTTFTRGAAEEAAIFQ
                     LARSTGFTVLASTNQEQLAAELKDLKHGIFTYAIINGLKGEADLKKDGKITVKEIELY
                     LNEIIPVLSEKYKGVQQYPQSFSRGMDFPLTISLN"
BASE COUNT         1526 a         1024 c         1137 g         1500 t
ORIGIN      
        1 ccagatcctt caaagcggaa ggaaatcaaa cttgaagaca tagaaatagg tgtaccggaa
       61 tatgtacctc cctcgcagga agaactaacg cataccggta agattacttt ctttaatacg
      121 gagaaaggat tcggcttcat caaagacctt gttacccagg aaagtgtttt tgtacatgta
      181 aataacctct ccgaacccgt taaagaaaac gacaaagtaa gttttgaagt ggagagtggt
      241 ccccgtggtc tgatggcggt taatgtaaag aaggtctgag ccgctttcat ttcaataacg
      301 gtagcaaacc gcatttacat tcatccctgc acctacagag gcaaacagaa ttacatcgcc
      361 aggttccagg gcatgttcat ccactttgtt tttcaccaca agatcataca aggtgggtat
      421 ggtagctacc gaactgttgc cgagccagtg tatgctcatg ggcattatat cattgggcgg
      481 ctcattcacg ccatatagct ggtacagcct tttgatcatg ccttcatcca ttttttcatt
      541 cgcctggtga atgaaaactt ttttcagctg ttgaatctca acgccactct gatcaaggca
      601 ttgcttcatg gcagccggca catatttcat ggcatattca tacactttcc ggcctttcat
      661 ctttatataa cgaacaccgg ggtcgctgcc cgggnnnnnn nnnnnntacg acatgcccat
      721 gttgatataa tccacctctt ctacggtatg gctttgcact gcagcaccga ttataccggg
      781 gccatcttca ttcacttctt tatactcaag cacagcagcc cccgcaccgt cactgaagat
      841 catgctgtca cggtcataca tatcaattac tcttgacaat gtttccgtgc caacgatcag
      901 tgcttttttg gcaatacctg ctttgaagta cgcatctgcc tgtatcaggc cctgcaccca
      961 gccggggcaa ccaaacaaaa tatcataggc gatgcaatta gggcttttaa tgcccaatgc
     1021 atgtttaatg cggctggcca gtgagggtac tgcatctgtc tgaatggtgt tcttaaaaac
     1081 gtttccgaaa ttatgcgcta cgatcagttg gtctatctct tcagggttaa caccgctgtc
     1141 tttaatagct gctgccgccg cctggctgcc aatgtcagag gtgttcagtt catcattggt
     1201 gtagcgtcgt tcatctatgc cagttatttg tttaaatttc tctaccacta ctgttggagg
     1261 agtatcaatg ggttcctgct tttctgtata aaaaagatgg gcagcaaaat ccttgttggt
     1321 catcaccaca gggggaatat agcaaccggt accggttatt atggaacgca ttatcattcg
     1381 aaatggttag tgattaaaac tacttcaatg aagttaaaca ggcaagtagt ttcctgttat
     1441 gatttatctc atctattatc cttacgacaa acgggtaaaa gatacgccat tgaatatgcg
     1501 gctaaggttg atgtccttgt tcattttcca ctgcgaatat tttgtttcaa tcacaattct
     1561 ccacaggttc tttgatttaa gttcagaata atcagttcct tcaataaata cacctgtcac
     1621 cgcctgtctg tctttaaaat ggatcgatac agcagattct ttacgcttgg cagattcaac
     1681 atacttctca atttgttcag aggtcatagt actaaatgct ttttgaaaca gggttactgt
     1741 tccggagggt tttaaaaaag ttgagaaata aatgcaggtg tagaagaaag aagaggaaga
     1801 taatctctta caagagcaat aaaactcaaa tatcggccga aaggtaagct aatttggctg
     1861 agcagtgtta ttctttttgt tactgttctg ccaattcatg tatttgttcg ctatttatac
     1921 ccttcttccg gtatagcaac ctgtttgcag tatattagct ttattgaaaa gtaaaatgac
     1981 acccatgaag atgttaaggc tgaaaggaat tacattttct ttttttattt ctttttctac
     2041 tttaaaggca atagcccagc cacgtcccgg cgatgccctg ttgcagatca atccgggcgg
     2101 tcatttgtca caaatatcag aagcggtaac cacggccgat ggaaaattta tcatcaccac
     2161 cagtactgat aaaaccattt gcatctggga tgtgcagcaa aaaaagatca ttgagcaaat
     2221 aagaggtccc aaatcggttt ttaaccaggg caaattatac tcacttgcta tttctccaga
     2281 caacaggctg ctggctgttg gcggatttct agccataggt acagaaacag acggtgacct
     2341 ggccggccag attcgcatct atgattttgc aaaaaggaaa caggtgcttc ggtttaaagc
     2401 gcatagtaat gttgtgacat cgcttcgttt ttctgccgat ggcaattacc ttgtatccgg
     2461 tgccaatgat agtactattg cttcatggaa agtttctggc accattgata agccatcttt
     2521 ttctctggta aaaaaagtag tcagggaaga cttttatgtg gaagatatcc aggtttccgg
     2581 taatgcagtt tttgttaccg acagaaaggg agtaaacaaa tacagcttac cctcactgga
     2641 acttgtttca gcatcttcgc agtatagtag tgatatgtac tgtattgcca ttaacagggc
     2701 tgccaatacc atagccatgg taagttttga tcaattggtg atatcagata ctgcacttaa
     2761 agataagcag gtaattgaac tggatagtta ttcaaaccgg gtagctattt caccgggtgg
     2821 caactacatt attacagagg gagaaaatag tacagtgcag ttgtatagca gggaggccag
     2881 ccagtttgta aaaactgttt cagcagattt taagggaggt aataccatgc tgggtatggg
     2941 atttctgagc aacagttctt tctttgttgc aggtggccgg gccaacatca tgcagtttta
     3001 tcggattgaa agcagaaatt ctaatatcat aaaaaccgat accgtactgg ccggcagtgg
     3061 tcaggctttc aatgccatat cagtacatga aaaaaaactg gctttgcgca gccttgaaaa
     3121 atacgaaggc tggtttgatt attcattgtt gccggaggca ggaaagttaa tgtcatttca
     3181 actttcagat tcaagtaaat accctgttaa acgcaatgaa aaaggcagtt tgagtgtcag
     3241 cgtcaatcgc nnnnnnnnnn nnnnnnnnnn atatttattc cggcaataaa ttaacgggta
     3301 cagtaaaaag agacggagcc agtggttatg gccataatgc ggttacgatc attaaaagca
     3361 actggataat ttcggcaggc agcgccggtt ttcttacact atacgattca ctgggcacgg
     3421 ccagggcttc atttgtaggc catgaaggtg atgtactgga cataagtgag tcagaggatg
     3481 gtaattttct ttattctacc gggtccgacc agactaccag gatgtgggat ctcagggagg
     3541 taaaaggaga tttggaattc aaatcatacg atcagcttga tcttacctgg aagaattttt
     3601 tcaaagacta ttttcctgag tatgatttta caagacctgg tgaaatgaaa aaagtgtatg
     3661 aaagattatt gagtatgggg agtaaagaga atgcagattt tctgttaatt ccacaacagc
     3721 tggaaccccg cctcaatatt ttcattgcaa agaacaacga gtgggtcatt tggaataata
     3781 agggttattt caaggcatca ccaaacgggg cctcttacat tggatggtat gtgtacaaag
     3841 gagaagacca gaacgcagag ttttataccg cagataagtt atttgataac tattataaac
     3901 cagggatcat taatgaattg gctttgtcgg atgaaagcac tgttactatc ctgcgaaaag
     3961 caggggatgc agcaaagacc agtattgccc agcaggtgag taatatgccg gtattaaaac
     4021 tggctgcacc atcagtcagt gaaaaagctg cacaaaagaa tattagcctg gttatcgatg
     4081 tggacaataa agattttatc agtgaactat tgttgtttca gaatggcaaa cggataactg
     4141 tcagtcctga tattttcagg ggtatttctc ttccttcaat aaccataccc gtagaattgg
     4201 ttaatggtga gaatactttc attgtatcag cgcttaacca aagcagggta gaaactacac
     4261 cgcttaaatt cagtatatcg ttcggcggta caaaagcttc aagctcctta tatataatgg
     4321 ctgtgggagt tgataaatat aaaaacagcc gttataatct caattatgct aaagcagatg
     4381 caaggggtat tgcagcacag ttagcttcct cctcttcaaa gatttttaag gatataattg
     4441 ttgactcgtt gtttgacgaa attgcaacaa cggaaaacat tgcagagcgg attaaccggc
     4501 ttaaaagtca gatcaaaccg gaagatgttt tcctgtttta ttatgcaggg catggcataa
     4561 tgagtgaccc ggcagatggt tccaaatcag atttctacct ggtattacat aaagtaacac
     4621 agatgcaggg gaatgatgaa gttttaaaaa caaatggttt atccgccagc cgcctgagag
     4681 atttattgtt ggaggtgccg gcgcaaaagc agcttatcct ttttgatgcc tgcaactccg
     4741 ggggagctct gactactttt actagaggtg cggctgagga ggctgctatt tttcaactgg
     4801 ccaggagtac gggatttaca gtccttgctt ctacaaacca ggaacagctg gcagcagaat
     4861 taaaagatct gaagcatggt atattcacct atgccataat taatggattg aaaggagagg
     4921 ccgatctgaa aaaagatggt aagatcaccg taaaagaaat tgagctctat ctcaatgaga
     4981 tcattcctgt tttgtctgaa aaatacaaag gagtgcagca gtatccgcaa tccttttcaa
     5041 gaggaatgga ttttccgtta actatcagtt tgaattaatg cccctgctga taatgaacaa
     5101 ggcttatctt tgccactcaa caaaatgtag cttatatgaa aaaaatattg tttgtattta
     5161 catctgcggc tttttttgct gcctggggcg gaggcgataa aaaagaatcg gctgaaaac
//
LOCUS       DAORLS010000224         5197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8734_length_5197_cov_2.620187, whole genome shotgun sequence.
ACCESSION   DAORLS010000224 DAORLS010000000
VERSION     DAORLS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8734_length_5197_cov_2.620187"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PK977_14660"
     CDS             <1..1143
                     /locus_tag="PK977_14660"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF19414.1"
                     /translation="ASIAAYEVVALGDDRFQSLAGQIKHLPKIPQQDLNNVNFQYAAI
                     LAFCKTGEAVTFPEGSMTAYRAMLDSLVKISGMNAAMKEASIQLADSVANHILRWSRK
                     DNYAETRTGERYNITKEEGRWIPTPPAYAQALEPNWRDIRPMVMDSADQFTPPRPPVF
                     NMTDKQSKFYQEVLAVKSAGDSLTDEQKHIAEFWDDLGNRTIVEGHVMFTAKKFSPPG
                     HWMNIVGIAAEKAKVDFSTTVYAYAKTAIAMFDGFIQCWDEKYRSNLVRPETVINKYI
                     DPDWRPYLQTPPFPEYTCGHSTVSASAAEALTDVFGDNFAYTDSSELEFGIKNRSFTS
                     FRQAAIENNWARFYGGIHFHHSCLISTDHGKMVGELVNQRLKMKIK"
     gene            1156..1734
                     /locus_tag="PK977_14665"
     CDS             1156..1734
                     /locus_tag="PK977_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19415.1"
                     /translation="MRKAAAILLITIYLVNLAGGAMLFEYLIQKNDRLLTERIDEGDY
                     DISQLVEVKVPLRLHYYSSSLHYERYYGEAEVDGRYYSYVMRKVFNDTVYLLCLPNHS
                     KAELKKGQASAGLIATDFGGTGNPVKGKGSEGKKQLSLTECCQHFNAFEIGSDVIFLK
                     SQLKEITVSINSCFIEPAGKPPRLNNSHSEAA"
     gene            1842..2801
                     /locus_tag="PK977_14670"
     CDS             1842..2801
                     /locus_tag="PK977_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRF19416.1"
                     /translation="MKQKLISNIIKPVIFIWLLVITGAVSAQTDIDGLMMEKNAFCVG
                     PMAGYSSWKNYWEGTLKRDNANLGTVSATQFSIMGNYGLTNKLNILFGLPYIKTKASA
                     GQLKGQKGLQDLALWVKWKAYSEKIGSGRLSLFLIGGYSLPVSDYTADFLPLSIGLES
                     KNLSVRGMADYQRGSWFATVSGTYVRRSNIKIDRDAYYTTEMHYSNEVEMPDMAAFNF
                     RSGYRDKGLIAEATADNMTTLGGFDITRNNMPFPSNKMSVTRIGFNFKYDMPFHPNLS
                     LTGNAFTTVAGRNMGQSSGFNAGIFYVLDFSKKERKNSSNPKN"
     gene            2807..4351
                     /locus_tag="PK977_14675"
     CDS             2807..4351
                     /locus_tag="PK977_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF19417.1"
                     /translation="MKKLHISIIFAVSVLFYSCSKDITETNLEYPVLDPASQDLNAGT
                     WRPILLTGPAEFSVPAPISTTSPDYIIQLNEIKSYQANITSKERKIVNYWGAGAVLRW
                     NEILRELVALHNLPPYQNPDGTYPVPSPNNPLAYPQFPFANPPYAARAYAYVAAAQYD
                     ALVATWHYKTLFNRSAPYNVDQSLNTLLPKSNIPSYPSEDAAVIGVTTELLKLLFPGD
                     QEFIQQKAEEHKKARILSGANVRSDLEAGEALGRSIAQKYITRARGDRAGAAVGTPAQ
                     WAQLENDCIARGEAPWMSLESPKRPPMLPLFGRVRTFLFDSLTMVTSIRPPAPPSTSS
                     QQFKDETNEVLYYSQNPTTERMKIVHFWADGVSTFTPPGHWNLIAANDFVKQRFSEVR
                     WARNMALLNMSLMDAAIACWDAKFVYYNPRPSQSDPQIKTLTGLPNFPSYISGHSTFS
                     GAAARILGHIIRSRANDYDNMANEASISRLYGAIHYRSDCEQGLITGRKIGDYAIARA
                     RTDGAE"
     assembly_gap    4436..4445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4446..>5197
                     /locus_tag="PK977_14680"
     CDS             <4446..>5197
                     /locus_tag="PK977_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylceramidase"
                     /protein_id="HRF19418.1"
                     /translation="KKNGNDPVPPPPPTPTNDVEMWLTKGDQSALLQKQTDILSFGTT
                     ANSFPNIDIDSATAYQSVDGFGYTLTGSSAYLIRRMGTAESAALLNELFGSNGIGVSY
                     IRISIGASDLSPAVFSYNDMPTGQTDPTLANFSLANDTVDLIPVLKQVLQINPNIKIM
                     GSPWSAPVWMKNNGSTIGGSLQPQYYAVYAQYFVKYIQQMQARGVPVHAVTVQNEPEH
                     GGNNPSMVMTATQQADFVKNHLGPAFQTAGIT"
BASE COUNT         1537 a         1042 c         1188 g         1420 t
ORIGIN      
        1 gcttctattg ctgcctatga agtagtggcg ctgggcgatg acaggtttca gtcgctggcc
       61 ggacaaatca aacatttgcc aaaaattccg cagcaggacc tgaataacgt aaattttcaa
      121 tatgccgcca tacttgcctt ctgcaagact ggtgaggctg ttacttttcc ggaaggcagc
      181 atgacagcat accgggctat gctggattca ctggtaaaaa tttcaggtat gaatgcggca
      241 atgaaggagg ccagtattca attagccgat tcagtagcca atcatattct tcgctggagc
      301 agaaaggata attatgcaga aacaagaact ggtgaaagat ataatatcac aaaagaagaa
      361 ggccgttgga tacccacacc tcctgcctat gcgcaagccc ttgaacctaa ctggcgggat
      421 ataaggccaa tggtaatgga tagtgctgac cagtttacac cgccaaggcc accggttttt
      481 aatatgacgg ataaacaaag taaattttac caggaggtgc ttgcagtaaa aagtgcaggt
      541 gatagtctta ccgatgaaca aaaacatatt gctgaattct gggatgacct gggtaaccgg
      601 acgattgtag aaggacatgt gatgtttact gctaaaaagt tttctcctcc cggtcattgg
      661 atgaatattg taggtatagc agctgaaaaa gcaaaggtgg atttttctac taccgtttat
      721 gcttatgcca aaacggccat tgctatgttt gatggattta ttcagtgttg ggatgaaaaa
      781 tacaggagca acctggtgag accggaaaca gtgatcaaca aatatattga cccggactgg
      841 aggccttatt tacaaacgcc accttttcct gaatatactt gcggacatag cactgtttct
      901 gcctcagctg ctgaagcatt aacggatgta tttggtgata attttgctta taccgattct
      961 tccgagctgg agtttggcat taaaaacagg tcttttactt ctttccgaca ggcggccatt
     1021 gaaaataact gggcaagatt ttatgggggc atacattttc atcattcctg tcttataagt
     1081 acagatcatg ggaaaatggt gggtgagctc gtcaatcaac gtttaaaaat gaaaatcaaa
     1141 taggaactta aaaaattgag aaaagcagct gccatattac tgattactat ttacctggtt
     1201 aacctggcag gtggtgctat gttatttgag tacctcattc aaaaaaatga ccggttgttg
     1261 acggaacgaa ttgatgaggg ggattacgac atttcacaat tggttgaagt gaaagtgcca
     1321 ttacggttac attattattc cagttcgctt cattatgaaa gatattacgg agaagccgaa
     1381 gtggatggtc gttattatag ctatgtaatg cggaaggtat ttaatgatac ggtctattta
     1441 ttgtgccttc ctaatcactc caaggcggag ttaaagaagg gacaagcaag tgccggatta
     1501 atagcaaccg atttcggagg tacaggaaac cctgtcaaag gaaagggcag cgagggtaaa
     1561 aaacagcttt cactgacgga atgctgtcag cacttcaatg catttgaaat tgggtcagat
     1621 gttatttttc tgaaaagcca gttgaaggaa ataaccgtaa gcataaattc atgttttatt
     1681 gagccggcag gaaagccgcc aaggttaaat aacagtcatt ctgaagctgc ctgatggtag
     1741 cgggacaatg ctgatggagg gcttgccttg ttttttgctt gcatcaaaca gggcagctaa
     1801 atgtctttca tcttaataac tgattattaa aaacatgatt catgaaacaa aaactaattt
     1861 ctaatataat taaaccggta atctttattt ggttgctggt aataacgggg gccgtatcgg
     1921 cacaaaccga tatagacgga ttaatgatgg aaaaaaatgc tttttgtgtg ggaccaatgg
     1981 cgggttacag cagctggaag aactattggg aaggaacatt aaaaagagat aatgccaact
     2041 tgggcactgt ttccgccacc caattcagta taatgggcaa ttacggactg accaataagc
     2101 tgaatatcct ttttggattg ccttatatta aaacaaaagc ttcagccggt caactgaaag
     2161 gccaaaaagg gttgcaggat cttgccttat gggtaaagtg gaaagcatac agtgaaaaaa
     2221 ttggttcggg ccggctttcg ctttttctga ttggcggata ttcattgcct gtatctgatt
     2281 acacagcaga ttttttacct ctttctattg gtcttgaaag caagaacctt tctgttcgtg
     2341 gtatggccga ctatcaaagg ggcagctggt ttgccactgt atcaggaaca tatgtacgac
     2401 gcagtaatat caaaatagac agggatgctt attacactac cgaaatgcac tacagcaatg
     2461 aagtagaaat gccggatatg gctgctttca acttccggtc cgggtacagg gataaaggat
     2521 tgatagctga agctacagct gataatatga ctacgttagg cgggtttgat attacccgga
     2581 ataatatgcc cttccccagt aataaaatga gtgtcaccag gattggtttc aatttcaaat
     2641 atgatatgcc ttttcatccg aatctttctt taaccggtaa cgcttttact acagtggcgg
     2701 gaagaaatat ggggcaatct tccgggttta atgccggtat tttttatgta cttgattttt
     2761 caaagaaaga gagaaaaaat tcttctaatc ctaaaaatta agaaagatga aaaagctaca
     2821 tatatcaatc atattcgccg tatcagttct gttttattcc tgttcaaagg atattactga
     2881 aactaatctt gagtacccag tgctggatcc ggcctcgcag gatctcaatg ccggcacatg
     2941 gaggcccatt ttgttaacag ggccagccga gttttccgtg cccgcaccca tttcaactac
     3001 cagtcctgat tatatcatcc aattgaatga gataaaaagt taccaggcta acattacctc
     3061 caaagaaaga aaaattgtaa actattgggg tgccggtgct gttctacgct ggaatgaaat
     3121 attaagggaa ttagtggctt tgcataacct gcccccttat caaaaccctg acggaactta
     3181 tccggttccc agtcccaata atccattggc ctatccgcaa tttccttttg ctaacccacc
     3241 ctatgctgca agggcctatg cttatgttgc cgcagcacaa tatgacgcat tggtggctac
     3301 ctggcattat aaaacgttat tcaacaggtc agctccttat aatgttgatc agtcgttaaa
     3361 tacactttta cccaagtcaa acattccctc ttatccatcc gaagatgcag cagtgatcgg
     3421 tgtaactacg gaattattaa aactcttgtt tcccggcgac caggaattta ttcagcaaaa
     3481 ggctgaagaa cataagaagg caagaatttt atccggtgct aatgtgcgca gtgacctgga
     3541 agctggtgaa gcattaggaa gatcaattgc tcagaagtac ataacccgtg caagaggtga
     3601 cagggcgggg gcagctgttg gcacaccggc tcagtgggca caattagaaa atgattgtat
     3661 cgcccgtgga gaagctccct ggatgagtct tgagtcgccc aaacgaccgc caatgctgcc
     3721 tttgttcgga agagtacgaa cattcttgtt tgactcactc accatggtga cctccattcg
     3781 tccaccagca ccaccatcta cttcatcgca gcagttcaaa gatgaaacaa atgaagtgtt
     3841 gtattacagc cagaacccaa cgacagagcg aatgaagatc gttcatttct gggcagacgg
     3901 tgtgagcaca ttcacacctc ccgggcattg gaatttaatt gctgccaatg attttgtaaa
     3961 gcaaagattc agtgaagtaa ggtgggcccg caatatggca ttgctaaata tgtcgttaat
     4021 ggatgctgcc attgcctgct gggacgccaa atttgtatat tataatccaa ggccatcgca
     4081 atcggatcca cagatcaaga cattgacagg attgcccaat tttccctcat atatatccgg
     4141 ccactcaacc tttagtggtg cggctgcaag gatactggga catatcatcc ggtcaagggc
     4201 taacgattat gataatatgg ctaatgaagc ttcgatatcc cgtttgtatg gagctattca
     4261 ttatagaagt gattgtgaac aaggtttaat aaccggaaga aagatcggag attatgccat
     4321 tgccagggcc cgaacagacg gggcggagta actattcagg aactaaaaat tctacagatg
     4381 aaaaaaagga caggactttt aatagtgagc ttttatactc tttttgtatg cggcgnnnnn
     4441 nnnnnccaaa aaaaatggga atgaccctgt gccaccgcca ccgcctacac ccactaatga
     4501 tgtggagatg tggctgacta aaggcgacca gtctgcttta ttgcaaaagc aaacggatat
     4561 actttcgttt ggtactactg ccaacagttt tcccaatatt gatattgatt cagcaaccgc
     4621 ttatcagtct gttgacggat ttggatacac attgaccgga agcagtgctt acctgatcag
     4681 gcgtatggga acggcagagt ctgcagcatt actcaatgag ttgtttgggt ccaatggcat
     4741 tggggtaagt tatatccgta tcagtatagg tgcttctgat ctgagtcctg ctgtttttag
     4801 ttacaatgat atgccaaccg gacaaaccga tcccacactg gctaatttta gtttggctaa
     4861 tgatactgtc gaccttatac ctgtattaaa gcaggttctc cagatcaatc ctaacatcaa
     4921 gatcatgggt tctccctgga gtgcaccggt atggatgaag aataatggca gtacaattgg
     4981 cggtagtttg caaccccagt attatgcagt ctatgctcag tattttgtca agtacattca
     5041 gcagatgcag gcaagaggtg tgccggtgca tgccgttaca gtacaaaatg agcctgaaca
     5101 cggaggaaat aatccgagta tggtgatgac cgctacccag caggctgatt ttgtgaagaa
     5161 tcatttaggg ccggcatttc aaacggctgg tattacc
//
LOCUS       DAORLS010000225         5193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8744_length_5193_cov_2.030946, whole genome shotgun sequence.
ACCESSION   DAORLS010000225 DAORLS010000000
VERSION     DAORLS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8744_length_5193_cov_2.030946"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK977_14685"
     CDS             complement(<1..598)
                     /locus_tag="PK977_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF19419.1"
                     /translation="MRIILFTLLLAFGFSSSFSQQPVIVSPQWLNENIKDPDLVILYT
                     GFVIKADYDREHIEGSRFLWPDWLAFDKPEGSMYAPDAITATKVLQELGINKNSKIVI
                     CHKGSDVTIAARMFMTLEHFGLKGQVSFLNGGIEAWKKAGYTVTNKLPEFKKGNYVAT
                     VAGTLVDKDYVLGALNTKSSEIVDARAKRWYDGDPTGNP"
     gene            complement(648..2672)
                     /locus_tag="PK977_14690"
     CDS             complement(648..2672)
                     /locus_tag="PK977_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF19420.1"
                     /translation="MKKIIAAIPLFSALLIAGQKNKKIDDLYFTLLKTAEAYYRHNQI
                     GEAKKMLLAVPEGKRTFEWLLLSARTDRSIYTLTAHNKAVVGIAVSKDGNLLATGSAD
                     NSIIIWDTDTYQQVKKIELHKGQITTLDFSPDGKSLVSGSTDKSLRLWDIVTGNEIRN
                     YNNEFKQGIYQCRFSNDGKMLGVVSWERGPNGVQGFAKILDAQSGALIKRFDTDDHPA
                     SAVKFSADNKKLYTGTWGFQIKQHDIASGNTDWNYDMREFDYYTAVQTMDISPDNKYV
                     VQGGKDNKIRMLNATDGKLMYEIESWQGHKEWVNGIRFSPDGSQFASVSDDGLLKVWE
                     TSSGKNLLTFRGHIAGLNQLAWHPDGKRIYTTSADNTVKIWDINQPGELAFTASVVGP
                     WNAPVSPDGKWMAPVNSDKYLALYSLNTGKPEIFLDSLSAFAAVFSADGQYLATGYRQ
                     INIYHTNTGKKILAGKGHTGVIYGMDYNSKLNLFVTTADNTVRVWNTTDTAAYKTVSN
                     IKSPFTVKFSPDGKNIYAGCADGKVKIISSTSWVVIDSLQSGTTIFNMAASNDGKYLL
                     TSGNAEVILWDFKKKKSISLAGHTKWVYGCAFHPTLPIAVTASYDRTLRFWDVEKGVN
                     TLTLFGFDHELYTVAFSKDGKRLIITETGGLAHILNLYLPVSKPDSPFVK"
     gene            complement(2682..>3175)
                     /locus_tag="PK977_14695"
     CDS             complement(2682..>3175)
                     /locus_tag="PK977_14695"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HRF19421.1"
                     /translation="QWIEIVNKSGFTKHGELVNFLKEKHGFTHGNANTVVHFAKQSHA
                     GAAENSDDLIAEQYKGKENLKPWYDKIMAEINKFGKDVEAAPKKAYVSLRRKKQFALI
                     QPSTKDRLDIGLNIKGVEPSGIVEDGKKWNAMCTHRIRIENEKAINKDLFNWIKQAYE
                     QAG"
     assembly_gap    3176..3410
                     /estimated_length=235
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3411..4142)
                     /locus_tag="PK977_14700"
     CDS             complement(<3411..4142)
                     /locus_tag="PK977_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF19422.1"
                     /translation="MILQFHIPSPPLTHYVELITYYKGYNPPHTIERLLPDGGIDLII
                     DLTSVPKYIYDNESLTEIQSCKRAWISGMRTEYISIQARAAESEMLVIRFRPGMAWSF
                     LHMPVLEIKDKVVDAELIFGNELLSFREQLLEQAEPVQKFTIAEQYLLKRIKNHFEIH
                     PAIRYCISQIGSNPSQASIREITHKTGYSSKHLISLFGKYAGINPKQYVSVLKFQQAV
                     QLLEKNPGQINWTALALDCGYYDQAH"
     gene            complement(4139..4507)
                     /locus_tag="PK977_14705"
     CDS             complement(4139..4507)
                     /locus_tag="PK977_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF19423.1"
                     /translation="MNLNKVTPLMYTKQLRETVDWYVNTLGFTCSDFVPGYGFARVQL
                     HGADFMFALPNENIPFEQPNFTGSFYINTNNADAWWEKLKDKCKVCYSIETFDYGMRE
                     FAVYDINGYLLQFGQPVIAE"
     assembly_gap    4539..4597
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4598..4900)
                     /locus_tag="PK977_14710"
     CDS             complement(<4598..4900)
                     /locus_tag="PK977_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19424.1"
                     /translation="MKKLIIITSLLACFNSFAQDSTKAKPKEWLGVLTLTDKFTIETN
                     WTSTDQAVVGEHFQRLIKMKESGTVILAGRMDLPLNDPEMKGLVIFYAKDEKEANQF"
     gene            complement(4935..>5193)
                     /locus_tag="PK977_14715"
     CDS             complement(4935..>5193)
                     /locus_tag="PK977_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF19425.1"
                     /translation="GLASQKNSINLYHMGIYADPKLLKWFQDAHAKVSPKKLDMGKSC
                     IRYKKPEDIPFELIGELASKITVDEWINMYETNLKDSRKKK"
BASE COUNT         1314 a         1089 c          881 g         1615 t
ORIGIN      
        1 tggggttacc ggttgggtca ccatcatacc atcttttggc ccttgcatca actatttccg
       61 atgatttcgt attcaacgca cccaacacat aatctttgtc taccagtgta ccagcaacag
      121 ttgccacata attacctttc ttaaactctg gtagcttatt tgtaacagta tagccagcct
      181 ttttccatgc ctcaatgcca ccgttcaaaa aagaaacctg ccctttaagg ccaaaatgtt
      241 ccagcgtcat aaacattctt gcagcaatag ttacatcgct ccctttatga cagatcacaa
      301 tttttgagtt tttattaata cctagttcct gaagcacttt agtggcagtt attgcatccg
      361 gtgcatacat actgccttct ggcttatcaa atgccagcca gtctggccaa agaaaacggg
      421 acccttcaat atgttcccgg tcataatctg cttttattac aaaccctgta taaagaatta
      481 cgagatcggg atcttttata ttctcattca gccattgcgg gctgacaatt acaggttgct
      541 gtgaaaaaga agatgaaaag ccaaatgcca gcaagagggt gaatagtatt attctcataa
      601 ttctgcattt atagaaattt acttctttct aatacctgca gatcaatcta tttgacaaaa
      661 ggcgaatcag gcttactgac cggtagatat aaatttaata tatgtgcaag acctccggtt
      721 tctgttataa taagacgctt accatctttt gaaaaagcaa cagtgtatag ctcatgatca
      781 aatccaaata aagtcagcgt attaactcct ttttctacat cccagaaacg aagcgtccgg
      841 tcataagatg ctgtaacggc aattggcaat gttggatgaa aagcacatcc atacacccat
      901 ttagtatgac cggccagtga aatacttttt ttctttttaa aatcccacaa aataacctct
      961 gcgttgccac tggtaagcag gtattttcca tcgttggatg cagccatgtt aaatatggtt
     1021 gtaccacttt gcagactatc gataaccacc caactggttg atgaaattat ttttacttta
     1081 ccatctgcac agcctgcata aatgtttttt ccatcaggag aaaattttac ggtaaatgga
     1141 ctttttatgt ttgatacagt tttatatgcg gctgtatcgg ttgtattcca caccctgaca
     1201 gtattatcag ccgtggttac aaacaagttc agtttgctgt tataatccat tccataaata
     1261 acaccggtat gtcctttgcc ggcaagtatt ttctttccgg tattagtatg gtagatattg
     1321 atttgcctgt acccggttgc cagatattgg ccatcagccg aaaatactgc tgcaaaagca
     1381 gaaaggctat ctaagaatat ttccggtttt cccgtattca aactatataa tgccagatac
     1441 ttatcactat taacaggggc catccatttt ccgtcagggg aaacaggtgc attccaggga
     1501 cctacaacac ttgccgtgaa agccaattca ccgggttgat tgatgtccca tatcttaact
     1561 gtattatcag ctgatgtagt ataaattctt ttgccgtcag gatgccatgc cagttgatta
     1621 agccctgcaa tatgtcccct gaatgtgagc aggttttttc cactgcttgt ctcccaaact
     1681 ttcagcaacc catcatcact aacgctggca aactgagacc catcaggaga aaaacggata
     1741 ccattcaccc attccttatg tccctgccag gattctattt catacatcag ttttccgtca
     1801 gttgcattca gcattcttat tttattatcc ttgccaccct gtaccacata cttattatcc
     1861 ggactaatat ccatagtttg cacagctgtg taataatcaa attcccgcat gtcataattc
     1921 cagtcggtgt taccagatgc tatatcatgc tgcttgattt gaaaacccca ggtacctgta
     1981 tagagttttt tattatcagc ggagaatttt actgctgatg cagggtgatc atctgtatca
     2041 aaacgcttta ttaaagcacc gctttgtgca tccagtattt ttgcaaaccc ttgtacacca
     2101 ttgggccctc tttcccagga aacaaccccc agcattttgc catcattact gaacctgcac
     2161 tggtaaattc cttgtttaaa ctcattattg taattccgga tttcattacc ggttacaata
     2221 tcccacaaac gcagcgattt atcagttgag ccgcttacca atgattttcc atccggtgaa
     2281 aaatcaagtg tggttatctg tcctttatgt aattcaattt tctttacttg ctggtacgta
     2341 tccgtatccc agataataat cgagttatct gcagagcctg tagcaagcag atttccatct
     2401 ttgctaacag ctattcctac cacagctttg ttgtgtgcgg ttagagtata tatgctccgg
     2461 tctgttcttg cactgagtaa tagccattca aatgttcttt taccctcggg tacagccagt
     2521 aacatttttt ttgcttcccc aatctggttg tgacggtaat atgcttcggc tgttttcaac
     2581 agggtaaaat atagatcatc aatcttttta tttttttgtc ctgcaatcag caacgcagaa
     2641 aagagaggta tcgcagcaat tatttttttc atataatatt tttatcctgc ctgttcgtaa
     2701 gcctgtttta tccagttgaa caagtcttta ttaatcgctt tttcattttc tatacggatg
     2761 cggtgcgtac acatcgcatt ccactttttc ccatcctcaa caatacctga aggctcaact
     2821 ccttttatat tcaatcctat atccaaccgg tctttagtgg agggttgaat caaagcaaat
     2881 tgttttttcc tccgcaaact tacataggct ttcttgggag ctgcttccac atctttgcca
     2941 aacttgttta tctcagccat tatcttatca taccagggtt tcaaattttc tttgcctttg
     3001 tattgttctg caatcaggtc atcactgttt tctgctgctc ctgcatgtga ttgttttgca
     3061 aaatgaacta ctgtattggc attcccatgt gtgaagccat gcttttcttt cagaaaattt
     3121 accagttccc cgtgttttgt aaaaccggat ttattgacga tttcaatcca ctgctnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgagcctgg
     3421 tcataataac cacaatccag tgccaaggct gtccagttta tttgacccgg atttttttcc
     3481 agcaactgta cagcctgctg aaatttcagc acacttacat attgtttagg attaatgccg
     3541 gcatactttc caaaaaggct gatgaggtgt ttgctgctgt agccggtttt atgtgttatt
     3601 tcacggatag acgcctgcga agggttactg ccaatttgtg aaatgcagta ccgaatggcc
     3661 ggatgaattt caaaatgatt tttgattctt ttcaacaggt attgttctgc aatggtaaat
     3721 ttttgcactg gttctgcttg ttccagtaat tgctccctga aggaaagcag ttcgttgcca
     3781 aaaatcagtt ctgcatccac taccttgtct tttatttcca gcaccggcat atgtaaaaaa
     3841 gaccaggcca tccccggacg aaaacgtatt acaagcattt cggattctgc agcccttgcc
     3901 tgtatggaaa tgtattctgt tcgcataccc gagatccatg cccgtttgca agattgtatt
     3961 tcagtaaggc tttcgttgtc gtaaatgtat ttgggaacac tggtaaggtc aattatcaaa
     4021 tcaattcctc catctggcag aagacgttca atagtatgcg gcgggttata ccctttgtaa
     4081 taagttatca gttccacata atgggtaagc ggtggggaag gtatatggaa ttgtaaaatc
     4141 attctgcaat aacgggttgc ccaaactgca ataaatatcc gttaatatca tacacagcaa
     4201 actcccgcat cccgtaatca aaagtttcaa tggaatagca cactttacac ttgtctttca
     4261 gcttttccca ccaggcatcg gcattgttgg tattgatata aaatgaaccg gtgaaattag
     4321 gttgttcaaa tggtatattt tcatttggca gggcgaacat aaaatcagcg ccgtgcagtt
     4381 gcactcttgc aaagccataa ccaggaacaa aatcgctaca ggtaaaaccc agcgtattca
     4441 cataccagtc tactgtttct cttagctgct tagtatacat cagcggggtg actttgttca
     4501 ggttcatggc taattacatt tacttaccgc cacaccatnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa ctggttcgcc tctttttcat
     4621 ccttagcata aaatattacg aggcccttca tctcagggtc gttaagtggt aagtccattc
     4681 ggccggccag tataaccgta ccgctttctt tcatttttat cagcctttga aaatgttcac
     4741 ctaccacagc ctgatcagtt gaagtccaat tggtttcgat agtaaactta tctgtaagag
     4801 taagcacacc cagccattct ttgggctttg cctttgtact atcctgtgcg aaagaattaa
     4861 aacaagccaa aagtgaagtg attattatta gttttttcat gtgctatttt tagcttttag
     4921 ggatttactg catattattt tttcttcctg ctgtctttca agtttgtttc atacatgtta
     4981 atccattcat caactgttat ttttgatgca agttctccaa tcaactcaaa gggaatgtct
     5041 tcgggttttt tataccttat acagcttttt cccatgtcta atttttttgg cgagactttg
     5101 gcatgtgcat cctggaacca tttgagcagc ttgggatcag cataaatacc catgtggtaa
     5161 agattaatag agtttttctg cgaggcaaga ccc
//
LOCUS       DAORLS010000226         5169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8791_length_5169_cov_1.761635, whole genome shotgun sequence.
ACCESSION   DAORLS010000226 DAORLS010000000
VERSION     DAORLS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8791_length_5169_cov_1.761635"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>219)
                     /locus_tag="PK977_14720"
     CDS             complement(<1..>219)
                     /locus_tag="PK977_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19426.1"
                     /translation="AGTGTSVTVTTSNFFRINNGGKYVHNTQRGHTNNLATRLSSVPG
                     TESGTFEFDVPGAGTYLVSASDRVYGNLI"
     assembly_gap    220..371
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<372..580)
                     /locus_tag="PK977_14725"
     CDS             complement(<372..580)
                     /locus_tag="PK977_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19427.1"
                     /translation="MRRYLLVIFLVVFSATVKSQITWDGGGDAVSWNDAANWVGNVVP
                     GAADNVLLDNSVVGGSYTVNLPTGAV"
     gene            complement(807..1010)
                     /locus_tag="PK977_14730"
     CDS             complement(807..1010)
                     /locus_tag="PK977_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19428.1"
                     /translation="MKKVFAILALTAVFAACNNSGDKKEETKDTTAAPATETVTPAPA
                     DTTTAPAADTTAAPAADTTKKSM"
     gene            complement(1129..2442)
                     /gene="der"
                     /locus_tag="PK977_14735"
     CDS             complement(1129..2442)
                     /gene="der"
                     /locus_tag="PK977_14735"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRF19429.1"
                     /translation="MSFTVAIVGRPNVGKSTLFNRLLEQRKAIVDDISGVTRDRQYGV
                     TDWNGKTFNVIDTGGFVHGSEDVFEKEIAKQVLVAVEEANAIIFMVDAATGMTNLDDA
                     MADVLRRSTKPVFLTVNKVDTNERMLEATEFYSLGFEHIFFIAAASGSGTGELLDAIA
                     ALITEDASEETREIAALPKFAIIGQPNVGKSSLLNALIGEERTIVSDIAGTTRDTIHT
                     HYNLFQKEFVLIDTAGIRRKAKVHEDLEFYSVIRAIKAVDEADVCMLLLDAEKGIAAQ
                     DLNIFSLAAKKGKGIVVLVNKWDLVKKETNTAKEYEAELKKRFAPFSDVPILFVSAKE
                     KTRIFKAIELALEVYDNRQRKIPTSQLNDIILKAIEAYHPPVVRGHPIKIKFVTQLPT
                     AVPSFAFFCNFPDDIKQPYKNYLENKLRENFDLTGVPVRLFFRKK"
     gene            complement(2522..>2916)
                     /locus_tag="PK977_14740"
     CDS             complement(2522..>2916)
                     /locus_tag="PK977_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRF19430.1"
                     /translation="KPILELLPEGEPYYTDDDISDLPTKFFVSELIREKIYELSQDEI
                     PYHTAVLVREFKEKASLVKIVADIIVHRETQKVILIGEKGSMIKKIGMAARKDIEGFL
                     QQKVFLELFVKVKPKWRENEMQLKEYGY"
     assembly_gap    2917..3028
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3029..3395)
                     /gene="era"
                     /locus_tag="PK977_14745"
     CDS             complement(<3029..3395)
                     /gene="era"
                     /locus_tag="PK977_14745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRF19431.1"
                     /translation="MKTGFVNIFGRPNAGKSTLLNALMGEKLAIVSPKVQTTRHRIKA
                     IMTDKDYQVIFSDTPGIIEPKYKLHEKMMQAVKSALEDADVALLIVDVNENWEECNTI
                     FEALKLKVPAVVVINKIDRA"
     gene            complement(3523..3594)
                     /locus_tag="PK977_14750"
     tRNA            complement(3523..3594)
                     /locus_tag="PK977_14750"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3560..3562),aa:Met,seq:cat)
     gene            complement(3698..>4204)
                     /locus_tag="PK977_14755"
     CDS             complement(3698..>4204)
                     /locus_tag="PK977_14755"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRF19432.1"
                     /translation="RIKVEVKDGILHISYDSKGMSWSTGNKKLKAYISFKQINKLDIS
                     GACDVYITGTLKTGELKIKQSGASDLKGKLDVNKLTVDLSGASDMMVSGSATQLSVEA
                     SGASDFKGYELVSETCDARASGASDIKITVNKELSAQASGASDVRYKGNGVIRDIKSS
                     GSSSVSKG"
     assembly_gap    4205..4355
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4421..>4813)
                     /locus_tag="PK977_14760"
     CDS             complement(4421..>4813)
                     /locus_tag="PK977_14760"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRF19433.1"
                     /translation="DILNANTGADVNIVGSIKSATLDIKANTGALIDGEVEVTTLKVS
                     QNTGSKINLSGRADNLSVDGDTGSKFKGEDMATSNCNVTVSTGAIVSVKAEKELQVKA
                     NTGGVVRYKGNASIKEIKTNTGGTVSKI"
     assembly_gap    4814..4860
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4861..5140)
                     /locus_tag="PK977_14765"
     CDS             complement(<4861..5140)
                     /locus_tag="PK977_14765"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRF19434.1"
                     /translation="MQKLLLSFLVMVFSVAVIAQKTINDPNAEKRTVGSFHGIEVGTG
                     IELTLTQGATEDVAVSAAKTEHRDRIVTKVENGILKIHYDSKLGSVNKV"
BASE COUNT         1187 a         1141 c          961 g         1418 t
ORIGIN      
        1 aataagattc ccataaactc tatcgctggc ggaaaccaga tatgtgcctg caccgggcac
       61 atcgaattca aaagtcccgc tttcagttcc aggaactgaa gacaatctag tggcaagatt
      121 attagtatgt ccgcgttgag tattgtgaac gtatttgcca ccattattaa ttctaaaaaa
      181 attcgacgta gttacagtta ctgaagtacc tgtgcctgcn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nactgcaccg gttggcaaat tcactgtata gcttcccccc actacagaat
      421 tatctagtag tacattatca gcagcacccg gaaccacatt tccaacccaa ttggcagcat
      481 cattccatga cacagcatca ccacctccat cccaggtaat ttgtgatttt actgttgccg
      541 aaaaaacaac aagaaatatg accagtaaat accttctcat tcgcaattat tttagttgtt
      601 gcggctacgg aaaatgaaaa ggcttttacg gagaagattg gatacggaaa attaaagctt
      661 accggtaaac aaatggttaa ggaagcggca caggggatgg tttgtggaaa actcgctgaa
      721 aacaaaaccc cggcctcctt cgacaagctc aggatgaccg gggaattatt tcttgcaaga
      781 aagtgtatcg taacgtcagc agacgattac atagacttct tggtagtatc agcagcagga
      841 gcagctgtag tatcagcggc aggagcagta gtagtatctg caggagccgg agttactgtt
      901 tcagttgcag gagcagctgt agtgtcttta gtttcttctt tcttgtcacc gctgttgtta
      961 caagcagcaa atacggctgt cagcgccagg attgcgaaaa cttttttcat tttttctggt
     1021 tttttcttgt tgggtttaaa aattcggtcg caaaggtagc cggaaatggc agtaaaccaa
     1081 atattttgcc ctttttttat accagctagc tgaaaaccag cggcttgcct atttcttccg
     1141 gaagaataac cgcaccggca cccctgtcag gtcaaaattt tccctcagtt tattctccag
     1201 gtaattctta tagggctgtt tgatatcgtc cgggaaattg cagaagaaag caaaactcgg
     1261 tacggctgtt ggcagctgtg tcacaaattt gatcttgatg ggatggcccc gcaccaccgg
     1321 cgggtgataa gcctcgatgg ctttcaggat aatatcattc agttgcgatg taggtatttt
     1381 acgctggcgg ttatcatata cttccagggc cagttcaatg gctttgaaga tcctcgtttt
     1441 ttctttggcc gatacaaaaa gaataggcac atcgctgaaa ggagcaaaac gttttttcag
     1501 ttctgcttca tactctttgg cggtattggt ttcttttttc accaggtccc atttgttgac
     1561 cagcaccaca atgccctttc cttttttagc cgccaggcta aagatgttca ggtcctgcgc
     1621 cgcaatgcct ttttccgcat ccagcaacaa catgcacaca tccgcttcat ccaccgcctt
     1681 gatagcacgg attacagaat aaaattccag gtcttcatgc actttggcct tgcggcgtat
     1741 gccggccgta tcgatcagga caaattcttt ctgaaaaaga ttataatggg tatgaatggt
     1801 atcacgggta gtgccggcaa tatcactcac aatggtccgc tcctccccga tcagcgcatt
     1861 cagcaaagat gacttgccca cattgggctg accgatgatg gcaaactttg gtaaagctgc
     1921 tatttccctg gtttcttccg atgcatcttc ggtaatgagg gcagcgatag catccaataa
     1981 ttcgccggtg ccgctgccgc tggctgctgc aatgaaaaaa atatgttcaa agccaaggct
     2041 ataaaactcg gtagcctcca gcattcgttc atttgtatct accttattca cggtaagaaa
     2101 aaccggtttg gtgctgcggc gcaatacatc ggccatcgca tcatccaggt tggtcatgcc
     2161 ggtggcagca tccaccataa agatgatggc attggcttct tccaccgcca ccagtacctg
     2221 tttggcaatt tctttttcaa acacatcttc actgccatgt acaaaaccac cggtgtcaat
     2281 gacattaaag gttttgccgt tccagtcagt aacaccatac tggcggtcac gggtcacccc
     2341 gctgatatca tccacgatgg ccttgcgctg ttccagcaag cggttgaaca aagtgctctt
     2401 gcctacgttg ggtcttccta ctattgctac ggtaaatgac ataataattg gttaatggct
     2461 gatagttcat tgttcatggc cgtttcaaaa ctatctatga accatgaact atgaacctgc
     2521 tttaatatcc gtattccttt aactgcattt cattctctct ccactttggt ttcactttta
     2581 cgaacagttc taaaaaaact ttctgttgca aaaaaccttc aatatccttt ctcgctgcca
     2641 tgccgatctt tttgatcata ctgcccttct ctcctatcag tatcactttt tgtgtttccc
     2701 ggtgaacgat gatgtcggcc actatcttca ccagcgatgc tttttctttg aattctctta
     2761 ccagcacggc tgtatggtaa ggtatctcat cctgcgacag ttcatagatc ttttccctga
     2821 tcagttcact gacgaaaaat ttagtgggca ggtcgctgat atcatcatcg gtataataag
     2881 gttcgccttc cggcaaaagt tccagtatcg gtttcannnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat gccctgtcga tcttattgat gaccactaca
     3061 gccggtactt tcaatttcag tgcctcaaat atcgtattgc attcttccca gttctcattt
     3121 acatctacaa tcagcagggc cacatcggca tcttcgagtg cactctttac agcctgcatc
     3181 attttttcat gcagcttgta tttaggctca atgatccccg gtgtatcaga aaagatgacc
     3241 tggtaatcct tgtcggtcat aatggctttg atccggtggc gggtcgtctg caccttgggt
     3301 gagacaatag ccagcttctc ccccatcaat gcattaagga gagtgctttt accggcattg
     3361 ggccggccga atatgttgac aaagcctgtt ttcatgataa aaacctgcag gtgcacacta
     3421 caggcagcaa gatgatgagg aggtaatcaa tccttgccag ttgatgtgca actgcttgga
     3481 aaatttaaaa aagccacctg caactgcgga tggctttctt tgttgcgaag ggagggatcg
     3541 aacctccgac ctacgggtta tgagcccgtc gagctaccgc tgctctactt cgcgatgttg
     3601 aggcgcaaag gtaagcttat accagttatg ttccaaagaa aaaacgccct gttttgcaac
     3661 agaacgttcc ttcatggctg taccccttta tatatgacta tcctttagag acgctgctgg
     3721 agccgctgct tttgatatcc cttatcacac cattgccttt gtaccgtaca tcactggcac
     3781 cgctggcctg ggcagataat tccttattca cggtgatctt aatatcactg gcgccgctgg
     3841 cccttgcatc acaggtttct gacactaact catatccttt aaaatcgctg gctccgctgg
     3901 cttccacact taattgagtg gctgaaccgg aaaccatcat atcagatgct ccactcaggt
     3961 caacagtcag tttattcaca tccagttttc ctttcaggtc gctggcacct gattgcttaa
     4021 tctttaactc accggttttt aaggtaccgg tgatataaac atcacaggcg ccactgatat
     4081 ccagtttatt tatctgctta aatgaaatat aagctttcag ctttttattg ccggtactcc
     4141 agctcatccc tttgctatca tagctgatat gcaaaatacc gtccttcact tctaccttga
     4201 tacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatact gcaaacaaaa tccagcaaca
     4381 ggattgataa ttttttcatt tttttctttt atcggttgaa ttaaattttt gatacagtac
     4441 cgcctgtatt tgttttgatc tccttaatgg atgcattgcc tttataacgt accacccccc
     4501 cggtattggc ctttacctgt aattcttttt ctgcttttac agaaacgata gcgccggtgc
     4561 ttacggttac attacaattg gaagtggcca tatcttcacc tttgaacttg ctgccggtat
     4621 ctccgtcaac actaaggtta tcagcacggc cggataaatt aattttggat ccggtattct
     4681 ggctcacttt cagggttgtt acctctactt ctccgtctat caatgcaccg gtattcgctt
     4741 ttatatccag ggtggctgat tttattgagc caacaatatt tacatcagcg cctgtattgg
     4801 catttaatat atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 ttactttgtt aacagaaccc agcttactgt cataatggat cttcaggata ccgttctcta
     4921 ctttggtaac gatacggtca cggtgctctg ttttagcagc gctgacagcc acatcttctg
     4981 tagctccctg cgtaagggta agttcaatac cggttcctac ttcaattccg tgaaaactgc
     5041 ccaccgttct tttttccgca ttggggtcgt tgatggtctt ttgagctata accgccacac
     5101 tgaataccat cactaaaaag gataataata acttctgcat aataattggt ttagaagtgg
     5161 tacgaaagc
//
LOCUS       DAORLS010000227         5126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8904_length_5126_cov_1.645632, whole genome shotgun sequence.
ACCESSION   DAORLS010000227 DAORLS010000000
VERSION     DAORLS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8904_length_5126_cov_1.645632"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>365
                     /locus_tag="PK977_14770"
     CDS             34..>365
                     /locus_tag="PK977_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19435.1"
                     /translation="MASIQAYWLGKLYQWKDKTNNIDLFNWGLAHYTAAEYLKSDSVF
                     TLYTERYPEDIFGYYWRAQSNAAIDTSLIEGLAVPHYIRVTEIGEKDKEKNKKMLLKA
                     YGYLGGYEA"
     assembly_gap    366..381
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(520..>1108)
                     /locus_tag="PK977_14775"
     CDS             complement(520..>1108)
                     /locus_tag="PK977_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF19436.1"
                     /translation="GEGWLKDKLKCKITIIPVANYNHDMPYTLPVKPSPNLNTQQAIL
                     LYPSTCMFEGVYLNHGRGTYFPFTVLGSPELKGLYSFSYTPTGIKGMAETPLFMDQVC
                     YGIDLRNYDVNQLRKTRQINLQWIMELYKAHPHKEKFFDSRLSNQMGTIEKLIGSGEF
                     RQQIIDGKSEKEIRASWEPGLSQYKEMRKKYLLYP"
     assembly_gap    1109..1197
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1198..1715)
                     /locus_tag="PK977_14780"
     CDS             complement(<1198..1715)
                     /locus_tag="PK977_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF19437.1"
                     /translation="MKKLSALFLFLSILSISFAQPAIKTGAEQTEKYLQLLKDKRVAI
                     MANQTSIIGSSHLVDSLSKLGVTIVKVFGPEHGFRGNASAGVLVADESDPVTGIPVIS
                     LYGKKNKPSKDDMADVDILIYDLQDVGVRFYTNINALARLMEACAENGKEMLLLDRPN
                     PNGYLIDGPVLD"
     gene            complement(1828..2265)
                     /locus_tag="PK977_14785"
     CDS             complement(1828..2265)
                     /locus_tag="PK977_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19438.1"
                     /translation="MEYAMEKKEYKMFQKKQTLQRLVDSIISQITPETAAGNSRIINE
                     VNPLLTIKTDPGVVEAVIKDLINILLENSLKRNIRISAKEYGNILLVHVKDYNNENSA
                     MSEMLPFSIIDKAKMISAYVGITRHFDNTTVVAFSFPNLPMAA"
     gene            2707..>3094
                     /locus_tag="PK977_14790"
     CDS             2707..>3094
                     /locus_tag="PK977_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HRF19439.1"
                     /translation="MNKIIFTEEFCQPENLYPFTLTRQLQDIRVGILTIREKWEMMLG
                     LASFDKKEDDYKDLGRSVNIDEAVGNDVCLMIHGNVLPTPKLVKAISRLKDGEFIATP
                     GGSSVAFRITKRDIVDKFKIKVGQAVT"
     assembly_gap    3095..3113
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3191..3898
                     /locus_tag="PK977_14795"
     CDS             3191..3898
                     /locus_tag="PK977_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19440.1"
                     /translation="MTEKRKSQPLAKTNKVIRPSQVFVEKGARVEFSVINASEGPVYI
                     GKNAEIMEGCLIRGSFAMGENSCLKMGSKVYGATTLGPGCVVGGEIKNSVLFGYSNKA
                     HDGYLGDSVIGEWCNMGAGTTNSNLKNNASDVKIWTPKGEMNAGMKCGVIMGDYSRTA
                     VNTSINTGTVIGVSCNVFGYGLTPKYIPSFSWGSEGVNRYEFDKALLDIADWKKLKGK
                     EVNEEEKSIMKYIFDKF"
     gene            3910..4122
                     /locus_tag="PK977_14800"
     CDS             3910..4122
                     /locus_tag="PK977_14800"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HRF19441.1"
                     /translation="MQKFEYKVLEVSAAGGFFSGGGVVNFQALSDKLNELGRQGWEVI
                     GSTDINRNYGDTRNVMIILKRPLNDS"
     gene            4128..4889
                     /gene="tpiA"
                     /locus_tag="PK977_14805"
     CDS             4128..4889
                     /gene="tpiA"
                     /locus_tag="PK977_14805"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRF19442.1"
                     /translation="MRKQIAAANWKMNLSYQEAETLLDEILEADIRLEFHQMAIFAVP
                     FPYLIMSRSEVEDEMNYEVAAQNCHHKKSGAYTGEVSVDMLHSIGVNYCIVGHSERRE
                     YNAETNAMLADKVNLCLEKAITPIFCCGEPLEIREAGTQNNFVAQQLKESLFHLSAVE
                     IKNIIIAYEPIWAIGTGKTASTAQAQEIHHYLRSVIAGKYGADIANEIPILYGGSVKA
                     GNAKELFASPDVDGGLVGGASLIADDFIEIIKALK"
BASE COUNT         1489 a          883 c         1118 g         1512 t
ORIGIN      
        1 actgccatta taaaacttgc aaagaaagca gatatggcat caatacaggc ttattggtta
       61 ggtaaactat atcaatggaa ggacaagact aataatattg atctgttcaa ctggggcctg
      121 gctcactata cagctgccga gtatcttaag tcggatagtg tgtttacatt atacacggaa
      181 agatatcctg aggatatatt tggttactat tggagagcac agtctaatgc tgctatcgat
      241 acttccttaa ttgagggttt agcagttcct cattatatac gagtgactga gataggagag
      301 aaggataaag agaagaataa gaaaatgctg cttaaggcat atggctacct gggcggatat
      361 gaagcnnnnn nnnnnnnnnn ngattatgtt cagtcgctta tgtggtttga gaaatacctt
      421 gaacttgaca gtgaaaatgc agatgccaaa cgatatgcag aaacgcttcg caaatggatt
      481 gaagaacaaa agaagtaata tattgcggta cttgctatat taagggtaca gcaagtactt
      541 tttacgcatt tctttgtact ggcttaaacc aggttcccaa ctggctctaa tttccttttc
      601 ggattttcca tcaataatct gctgacgaaa ttcgccagag ccaatcaatt tctcaatggt
      661 tcccatctgg ttactcagcc tggagtcaaa gaatttttct ttatgtgggt gtgccttgta
      721 caactccatt atccattgca gattgatctg cctggttttg cgaagctgat ttacatcata
      781 atttctgaga tcaattccat aacaaacctg gtccataaag agaggcgttt ccgccatccc
      841 tttaattcct gttggggtgt aagaaaatga ataaaggcct tttagctcgg ggctgccaag
      901 aacagtgaaa ggaaaataag tgcccctgcc atgattcaga taaacacctt caaacataca
      961 ggtagaggga taaagcaaaa tggcctgctg tgtgttcagg ttgggtgagg gttttaccgg
     1021 tagggtgtaa ggcatgtcat ggttataatt agccaccggg atgattgtga ttttgcattt
     1081 aagcttgtct tttaaccagc cttcaccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata
     1201 tccaaaaccg gtccgtcaat aaggtatccg tttggattgg gcctgtccag cagcaacatt
     1261 tcctttccat tttcagcaca agcttccatc aggcgggcta aagcattgat attggtataa
     1321 aatcggactc caacatcctg caggtcatag atcagaatat ctacatctgc catatcatct
     1381 ttgctgggtt tgttcttttt gccgtaaagt gagataaccg ggataccggt tactgggtct
     1441 gattcatccg ctaccagaac accggcgctg gcattgccac ggaaaccatg ctctggtcca
     1501 aacactttta caatagttac ccccagtttt gacaggctgt caaccaaatg tgacgaacca
     1561 atgatgctgg tttgattggc catgatggcc actcttttgt ctttaagtag ttgtaaatat
     1621 ttttctgttt gttctgcccc ggtttttatg gcaggttgcg caaaactaat cgagagtatg
     1681 cttaagaaaa ggaaaagggc cgacaatttt ttcatttgtt gaaatttgta agcccaaaat
     1741 actgaattgt ttgatgtagt ttatccgctc agcaagtttt agctatatag taacgggcaa
     1801 tgccacctgt ctgttactca aattgactta tgcagccatc ggtagattag gaaaactaaa
     1861 tgcaacaact gtagtgttgt caaaatgcct ggtaatccct acgtaggcgc ttatcatttt
     1921 tgctttgtca ataatagaaa acggcagcat ctctgacatg gccgagtttt cattattata
     1981 atccttaaca tgtactaata gtatgttgcc atattctttg gcggagatac gaatattccg
     2041 tttcaaacta ttttccagta atatgtttat taaatctttt attacagctt ccactactcc
     2101 cggatcagtc tttattgtta ataagggatt tacttcatta attattcggg aatttccagc
     2161 tgctgtttca ggtgttattt ggctgataat actatctacc agtcgttgaa gtgtttgctt
     2221 tttctgaaac attttgtact cttttttttc cattgcatat tccatatgat agctttttgt
     2281 ataagttttt catgcgggta cggattgatc atgtttttca atgggtatag aataagctta
     2341 aggacaggtg cggaattaat cagagggtac tggatcctaa acaggatatt ggacattgtt
     2401 gtttctcata ggccccagcg gggaaatatt gttgtgtctt tctggataca aagtaactgc
     2461 ggtttaaaga cgtaaaaaat agaacctttt gcttttggtt agtagttgtc tgactactgg
     2521 ttactataat tacttcgatc ttttgtaggt aattaccttt aaataatatc agcacatgag
     2581 tgatgatcag cctatttgat gatttcggtc attctcccgg ctgatattgc atatatatct
     2641 tgttgtatta taggccccaa agtcaggggt gaacatcttt gcttaatttt acgtaaccac
     2701 ttagttatga ataaaatcat ttttactgag gaattctgtc agcctgaaaa tctataccct
     2761 tttacgctaa cccggcaact tcaggatatc cgtgttggaa ttttgaccat cagggaaaaa
     2821 tgggaaatga tgcttggact ggcatcgttt gacaaaaagg aagatgatta taaagatctc
     2881 ggccggtcag tcaatattga tgaagcagta ggtaatgatg tttgtcttat gatccacggc
     2941 aatgtgttgc caactcctaa attggtcaaa gctatttcaa gattaaaaga tggcgagttt
     3001 atagctacgc cgggaggcag cagcgttgct tttcgtatca ctaaacggga cattgttgac
     3061 aagtttaaaa taaaagtcgg gcaagccgtt acttnnnnnn nnnnnnnnnn nnnaaccaat
     3121 aaagaccatt caatatccgt aggacctatt tcagttaaat gactgggcca taaaacagga
     3181 ttttgcttta ctgaccgaaa aaaggaaatc acagcccttg gcaaaaacta acaaagtgat
     3241 caggccctca caggtatttg ttgagaaagg tgcaagagtt gagttttcag ttattaatgc
     3301 atctgaaggt cccgtttata ttggcaagaa tgccgaaatt atggagggtt gcctaataag
     3361 gggctccttt gcaatggggg aaaattcatg cctgaaaatg ggaagcaaag tgtatggcgc
     3421 tactacgttg ggccctggtt gtgttgttgg aggtgagatt aaaaattcgg tgctatttgg
     3481 gtatagtaat aaagcacatg acggctacct gggagattcg gtaataggag aatggtgcaa
     3541 tatgggagct ggaacaacta acagcaacct gaagaataat gccagcgatg taaaaatatg
     3601 gacaccaaaa ggggaaatga atgccggtat gaaatgtggg gtaatcatgg gggattattc
     3661 ccgtacggcg gtaaatacat ctatcaatac aggtacagtg ataggtgtta gctgtaatgt
     3721 ttttggctac ggtcttacac caaaatatat tcccagtttt tcgtggggca gtgaaggagt
     3781 aaaccggtat gaatttgata aggcattact tgatattgca gattggaaga aattaaaagg
     3841 taaagaagtt aatgaagagg agaaaagcat tatgaagtat atatttgaca aattttaaag
     3901 ttttgccaca tgcaaaagtt tgaatacaag gtgctggaag tatctgcagc cggtggtttt
     3961 tttagtggag gtggtgtggt aaatttccag gctcttagtg ataagctgaa tgaactggga
     4021 aggcagggat gggaagttat tggcagtacc gatataaacc gaaactatgg tgatacaaga
     4081 aatgtaatga tcattttaaa aaggccttta aacgatagtt aataattatg agaaaacaaa
     4141 ttgcagcggc gaactggaaa atgaacctga gctaccagga agcggaaaca ctattggatg
     4201 aaatactaga agcagatatc aggctggaat ttcatcagat ggctattttt gccgtccctt
     4261 tcccctacct tattatgagc agaagtgaag tggaagatga gatgaattat gaagtggcag
     4321 ctcaaaactg tcatcataaa aaatccggtg cctataccgg tgaagtgtca gttgatatgc
     4381 ttcattccat tggagttaat tattgtattg tcggccatag tgaacgaaga gagtacaatg
     4441 cagagactaa tgccatgctg gcagataaag tgaatctttg tttagaaaaa gccatcactc
     4501 caatcttttg ttgcggcgaa ccgcttgaaa tccgggaagc cggtacacaa aataatttcg
     4561 tagcgcaaca acttaaagag tctttatttc atttgtcagc tgttgaaatt aagaatatta
     4621 ttattgccta cgagcctatc tgggccatcg gaaccggtaa aaccgcttcc acagcacagg
     4681 cacaagaaat acaccattat ttacgttctg ttattgccgg gaagtatggt gctgacattg
     4741 ccaatgaaat tccgattctc tatggtggca gtgtaaaggc gggtaatgcc aaagagttgt
     4801 ttgccagtcc cgatgtagat ggtggattgg taggtggcgc ttcgctaatc gctgatgatt
     4861 tcattgagat tattaaggca ttgaaataac ttcagaacgg cggattatta attttaactg
     4921 ttcaggtatt tctgctcctt ttcacatagt gctgcaacga aaaaaccagg tactgcttag
     4981 tttcctgaaa gtgagggcaa ttgtaaacgg taaagaaatc tatccgttac ctgataacaa
     5041 accagtggta atacccgtgc agatgaataa tcccaggctc gtaattaccg acggttatca
     5101 tatctctagg ccattaaaaa tggttt
//
LOCUS       DAORLS010000228         5125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_8906_length_5125_cov_1.828600, whole genome shotgun sequence.
ACCESSION   DAORLS010000228 DAORLS010000000
VERSION     DAORLS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8906_length_5125_cov_1.828600"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK977_14810"
     CDS             <1..216
                     /locus_tag="PK977_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19443.1"
                     /translation="PLKYYIPGASTDYKSAMELRQLKYQVDEANKQIRLLQQFDAGLR
                     NGPLKGNNTVTLDTTILKVPKAEISQD"
     gene            228..638
                     /locus_tag="PK977_14815"
     CDS             228..638
                     /locus_tag="PK977_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRF19444.1"
                     /translation="MFNTKNKTDMQPTNGNGNGTTLIGAGTTLKGDISSSSDLRIDGT
                     IIGNITSSAKIVIGSSGVVEGDITGNQADIVGKVSGNIRAKELLQLRGDAVVNGNIYA
                     GKLQMEPTATFNGQCHMGANIVEMNNNEQQAAAR"
     gene            613..840
                     /locus_tag="PK977_14820"
     CDS             613..840
                     /locus_tag="PK977_14820"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRF19445.1"
                     /translation="MNNKQPPDSNKQFFLRYASLGSQLLAAIGLGVFLGLKADQWLHT
                     SPVLACVLPLLVLSGIFIKIFRETSRKKNNE"
     gene            833..1117
                     /locus_tag="PK977_14825"
     CDS             833..1117
                     /locus_tag="PK977_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19446.1"
                     /translation="MNKSFFDWAKPLIGLFIVVTIVAVAGKEWLAHYHIDWRVVAVGN
                     FLLLDVGLTGLFIMMKTLKSSNPQAFVRAMYGSFIIKFFVLAVAAFVISW"
     gene            1223..>1441
                     /locus_tag="PK977_14830"
     CDS             1223..>1441
                     /locus_tag="PK977_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19447.1"
                     /translation="MPKKEVPVDHLQQYLPDGTGEAVTNYLHQYKVHLTITRERKSIL
                     GDYRHRTHHANHRISINGSLNKYAFLVTL"
     assembly_gap    1442..1494
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1495..1841
                     /locus_tag="PK977_14835"
     CDS             <1495..1841
                     /locus_tag="PK977_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19448.1"
                     /translation="GKEWKMIFGQLLQQFLQHHTFPADIEKELLLSLKNPAASSCAEE
                     GLIRVLRNYDAKKDNHHLVEELPAGSIFRLKDGRVFKKGEKIRKRFKCTELQTGKLYL
                     FSPVYEVEWLQA"
     gene            1916..2467
                     /locus_tag="PK977_14840"
     CDS             1916..2467
                     /locus_tag="PK977_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19449.1"
                     /translation="MKKIILGFAAMTGVLIANAQTNTGDWMVGGNFRLNTADNNTQIS
                     FTPNAGIFVVDNLAIGGNFGLSYSKSGNNKFTSFNVGPFVRYYFTTESQAVRPILHGT
                     FNYLSTKSKIANTASSTNTGVNFFIGGGAAAFISNNVSIDALLGYDRTKYKNFDGSGG
                     FAFNIGFQVYLLKDQVDKVKGKK"
     gene            complement(2607..3089)
                     /locus_tag="PK977_14845"
     CDS             complement(2607..3089)
                     /locus_tag="PK977_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF19450.1"
                     /translation="MDPIIRPIQPADNPIIAKIIRDTLAEFGANHPGTVYFDPTTDTL
                     YELFQKERSVYNIVELDGKIVGGGGIYPTDGLPGDTCELVKMYLLPEARGTGLGSQLI
                     ALCLQQAKANGFKKVYLETMNELKAALKVYARLGFTYLDGPMGNSGHYGCPLWMIKEL
                     "
     gene            complement(3342..4184)
                     /locus_tag="PK977_14850"
     CDS             complement(3342..4184)
                     /locus_tag="PK977_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19451.1"
                     /translation="MKTYFVLPSLIAIISLYGCRKESRINTTTAGERLKRISQYRPSL
                     ASTLFNDYYYDLQNRVIEIIEYEKTNTGDSIPIDRALFYYTGADSLPARLHKLTNNIW
                     HDFVYDAQKKLIKDSITKIINPLPITGRATFEYYTNYHLCYHSQPSSGSGSTTKDSLI
                     FLNNNLKYMRTSFDIYYSFPPIEYLEHDYEQYDNKKNPVYFLNIGEFYYNYRIANTGV
                     SIPAEIFYSKNNPIKRNSYDSFMNTHFQSGIVSYTYNTNDLPIKKVEVKNNDTIYVTT
                     YMYE"
     gene            complement(4236..4538)
                     /locus_tag="PK977_14855"
     CDS             complement(4236..4538)
                     /locus_tag="PK977_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19452.1"
                     /translation="MANKKALKFTFIVNFRGGIYCAQVDATNVNESILQWINQIEKGR
                     DQIKFLGNKIISELKKEVQHDYNIPVPLNGLKNIWFVSFSTRKGSFLINIVKTASK"
     gene            complement(4681..>5125)
                     /locus_tag="PK977_14860"
     CDS             complement(4681..>5125)
                     /locus_tag="PK977_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_079473658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HRF19453.1"
                     /translation="VIPTHFHDDCLGGLNAFDKNIIPSYANFKTIELATKNKLAVPKN
                     GFSDSLILKVGVETIIAQYFGEGHTKDNIVGYYPKENILFGGCLIKELDASKGYLGDA
                     NTAAWSGTVEKIKKQYPDIKIVVPGHGEHGNKSLLDYTINLFRAQ"
BASE COUNT         1447 a          978 c         1108 g         1539 t
ORIGIN      
        1 cctttgaagt attatatacc gggtgccagc accgattata aatctgctat ggagcttcgc
       61 cagctgaagt accaggtgga tgaagccaat aaacagatcc gtttattgca gcagtttgat
      121 gcaggcttac ggaacgggcc tttaaaagga aacaacactg ttaccctgga taccacaatt
      181 ttgaaagtac caaaagcaga aatttcacag gattaaccaa catagttatg tttaatacta
      241 aaaacaaaac cgacatgcaa ccaaccaacg gcaatggaaa cggcactacc ttaattggcg
      301 ccggcaccac cctgaagggt gatatcagca gcagcagtga tctacgaatt gacggcacca
      361 tcatcggtaa tattaccagt tctgccaaga ttgtgattgg cagcagcggt gttgtagaag
      421 gtgatatcac cggcaaccag gctgatattg tgggaaaagt atccggcaat atccgggcta
      481 aagagttgtt acaactaaga ggtgatgccg ttgtgaacgg aaatatttat gcaggtaagt
      541 tgcaaatgga gcccacagcc accttcaatg gccagtgcca tatgggagcc aatatagtag
      601 agatgaataa caatgaacaa caagcagccg ccagatagta acaaacaatt cttccttcgt
      661 tatgccagcc tcggttcgca gttgctggcc gctataggac tgggtgtttt tttaggatta
      721 aaagcagacc agtggctgca tacatcccct gttctggcct gtgtcttacc tttgctcgtc
      781 ctgtcaggga ttttcatcaa gatcttccgg gaaacaagca ggaaaaagaa taatgaataa
      841 gagctttttt gattgggcca aacctttaat cggacttttc atcgtggtga ctattgttgc
      901 tgtggccggc aaagaatggc tggcacatta ccatatcgac tggcgggtag ttgccgttgg
      961 aaacttttta ttgctggatg tgggccttac cggtttattt atcatgatga aaacactgaa
     1021 gtcttctaat ccacaggcat ttgtcagggc gatgtacggc agtttcatta tcaaattctt
     1081 tgtgctggct gttgctgcat ttgttatatc atggtaacta aaaaagcagt caataaagaa
     1141 ggccttgtgg tttgtgctgc tttatacatt gcttatactt ttcttgaagt aaggatactg
     1201 accaaactgc tgagggagaa aaatgcctaa gaaagaagta ccggttgatc acctgcagca
     1261 atacctgccc gacggcaccg gtgaggcggt gacgaattac ctgcaccagt acaaagtaca
     1321 ccttaccata acaagggaac gaaaaagtat tttaggtgat taccgtcacc gcacccatca
     1381 tgccaatcac cgtatcagca taaacggaag cctgaataaa tatgctttcc tggtcaccct
     1441 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatggca
     1501 aagaatggaa gatgatcttc gggcaactgc tgcaacaatt tttacaacat catactttcc
     1561 ctgctgatat tgaaaaggaa ttattgcttt cgctgaagaa ccctgccgcc agcagttgtg
     1621 cggaagaagg actgatacgg gtattaagaa actatgatgc gaaaaaggac aatcatcacc
     1681 tggtggaaga gttaccggcc ggcagtattt ttcgactcaa ggatggccgg gtatttaaaa
     1741 aaggagaaaa aattcgcaaa cggtttaaat gtacagaact gcaaacgggc aagctctatc
     1801 tcttcagccc ggtatatgaa gtggagtggc tgcaggcctg aactgttaaa acaacctgat
     1861 cagtttcctc attaattttt gctgctgtac ctttgcggcc acaaaaaata atcatatgaa
     1921 gaaaatcatt ttaggatttg cagcaatgac aggagttttg atcgctaatg cacaaaccaa
     1981 taccggcgac tggatggtag gtggcaactt tcggttgaac accgctgata acaacacaca
     2041 gatcagcttt acaccgaatg caggcatttt tgtagtggat aatcttgcca tcggtggcaa
     2101 ctttggtctc agctattcaa agtcgggtaa taacaagttc acctcattta atgtaggtcc
     2161 ttttgtacgt tattatttca ctacggagtc gcaggctgtc cgccccatac tgcacggaac
     2221 gtttaattac ctgagcacta aaagcaagat tgccaacacc gcttcttcta ccaataccgg
     2281 tgtgaacttc tttataggtg gtggtgctgc agctttcatc agtaacaatg tttccattga
     2341 tgccctgctt ggttacgacc gtacgaagta taaaaacttt gatggtagcg gtggttttgc
     2401 cttcaacatc ggcttccagg tgtacctgct gaaagaccag gtggataaag tgaaggggaa
     2461 aaagtaagga ttagccactg aggcacagaa tatatttccg gcatttctaa ccacagatta
     2521 cacggattac tttcacggat ttcacagggt tactaataac cgtgtgatct gtgtttaatc
     2581 tgtataatct gtggttttaa tttatttcag agctctttta tcatccacag cgggcagccg
     2641 taatgaccgc tgttgcccat cgggccatca agataggtaa agcccaggcg ggcatatact
     2701 ttcagtgcag ctttcagttc gttcatggtt tccaggtaca cttttttaaa gccattggct
     2761 ttggcctgct gcaggcaaag ggcaatcagt tgtgagccga ggccggtacc ccttgcttcg
     2821 ggcagtaaat acatttttac gagttcacaa gtatcgccgg gcaatccatc tgtagggtag
     2881 atgccgccgc cgccaactat ctttccatcc agttcaacaa tattgtaaac tgacctctct
     2941 ttttgaaaca gttcgtacaa ggtgtccgta gttggatcga agtatactgt gccgggatga
     3001 ttggcgccga attcggccag cgtatccctt atgattttag caataatggg attgtcggct
     3061 ggctgtatgg ggcgtatgat ggggtccatg ttgtaaagtt atgtcttcag agggcatggg
     3121 tatttgtctg aaccaggatt gggatagatt atctggattt cagagatttg aaattctcct
     3181 ttaatgctag ttactttaag tgcatagggc tgcgagacta caagtctcgc agagccatgc
     3241 tgtgttcggc ctaagtgttt ttaaagtaat aagtgattga tcgaatggcg gaacaaagtg
     3301 ctgaaagatt tctgattttt gaagggaagt taaaggctga attactcata catatatgta
     3361 gtaacataaa tcgtgtcgtt gtttttcact tcaacctttt ttataggaag atcattagta
     3421 ttatatgtat atgatactat accggattga aaatgtgtat tcataaagga atcataagaa
     3481 tttcttttta taggattgtt tttgctataa aaaatctcag ctggtataga tacccctgta
     3541 ttcgcaattc tatagttgta ataaaactcc cctatgttta aaaagtacac aggatttttt
     3601 ttattgtcat attgttcgta atcgtgttcc aggtattcga tagggggaaa agaatagtaa
     3661 atgtcaaaag aagtcctcat gtattttaaa ttattgttca ggaatatcaa tgagtctttc
     3721 gttgtgctgc ccgacccgct tgaaggctga gagtgataac agagatggta attagtataa
     3781 tattcaaatg tggcacgccc cgtaattgga agcggattaa ttattttagt aattgaatcc
     3841 ttaatcaact tcttttgtgc atcataaaca aagtcatgcc atatgttgtt tgtcaattta
     3901 tgcaatcttg caggtaaact gtcagcaccg gtatagtaaa ataaagctct gtctatagga
     3961 atgctatccc cggtgtttgt tttttcatac tcgattattt ctataaccct gttttgtaaa
     4021 tcgtagtaat aatcattaaa gagagttgaa gctaatgaag gacgatattg acttattcgt
     4081 tttaatcttt cacctgcggt agtagtgtta atcctgcttt ctttcctgca accatacaga
     4141 gaaataatgg ctattaaaga aggtaaaaca aaataagttt tcactgaaaa tactttttta
     4201 aaattgaata aaaattttcc gcttcataag ctagcttatt tggaggctgt ctttacgatg
     4261 ttaataagaa atgatccttt tctcgtagag aaacttacga accaaatatt tttaagtccg
     4321 tttaagggaa caggtatgtt gtaatcatgt tgaacttcct tttttaattc gctgataatt
     4381 ttattgccca aaaattttat ctgatctcgt cccttttcaa tttgatttat ccattgcaaa
     4441 atggattcat taacgttagt agcatcgact tgggcacaat atattcctcc acggaagttt
     4501 acaataaatg tgaatttcaa agccttctta ttcgccatag tgtagggcat ctgtttttgc
     4561 tatcagtcaa cttgtaaata tgtattattt cttttcaaat ttaccaagat tcaatagata
     4621 atgaagcgaa gctggttttg aaagcggcct ggtgtggaag ggaaaaggat gtttagttct
     4681 ttattgtgcc ctgaataagt taatggtgta atcaagcagg gatttgtttc catgttctcc
     4741 gtggccgggt actacgattt ttatatccgg gtattgcttt ttgattttct ccacagtgcc
     4801 tgaccaggct gcagtattgg catcgcccag gtaaccttta ctggcgtcta actcttttat
     4861 taaacaaccg ccaaacaaaa tattttcttt ggggtagtag ccgactatat tgtcttttgt
     4921 atgtccttct ccgaaatatt gggcaatgat cgtttcaacg ccgactttta aaatgagtga
     4981 atcgctgaaa ccgtttttgg ggacagccag cttgtttttt gttgccagtt cgatggtttt
     5041 gaaattggca taagacggga taatgttttt gtcaaatgca ttcagccctc ccaggcaatc
     5101 atcgtggaaa tgagtgggaa taacg
//
LOCUS       DAORLS010000229         5075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9004_length_5075_cov_2.188645, whole genome shotgun sequence.
ACCESSION   DAORLS010000229 DAORLS010000000
VERSION     DAORLS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9004_length_5075_cov_2.188645"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..461
                     /locus_tag="PK977_14865"
     CDS             102..461
                     /locus_tag="PK977_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HRF19454.1"
                     /translation="MDAARNFIRAALDGKYREAKDYLLQDSVNLNWMDIAERNYLKAD
                     QDTKDGYRASSINMHEVTPVNDSTAIIIYSNSFKNDHDTLKVVKLNGQWLVDLKYLFE
                     HDRDTLIKKPVINDTIK"
     gene            458..829
                     /gene="crcB"
                     /locus_tag="PK977_14870"
     CDS             458..829
                     /gene="crcB"
                     /locus_tag="PK977_14870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRF19455.1"
                     /translation="MIKNILLVGLGGGAGSILRYLCQRWFAEVYSHSFPWGTFAVNIT
                     GCFLIGVFWGLTFKTFDSNEHWKLFLMTGICGGFTTFSAFTLEGIGLIREQKLGMFFL
                     YVAGSVVAGLALTLVGMKLTR"
     gene            834..1376
                     /locus_tag="PK977_14875"
     CDS             834..1376
                     /locus_tag="PK977_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRF19456.1"
                     /translation="MTTVLHPWHGVNHGEKAPATVNALIEIPQGSRSKYEVDKATGLL
                     KLDRVIYSSFQYPINYGFIPQTLGLDNDPLDILVICSQSIQSLCLVEANVIGNMQMID
                     SGQEDDKIIAVAAKDPSVNHITRMEELPQHFLLELKNFFEQYKVLENKKVEIDTFQDQ
                     LIAYKIINDSIHFYKETFKK"
     gene            1404..1823
                     /locus_tag="PK977_14880"
     CDS             1404..1823
                     /locus_tag="PK977_14880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF19457.1"
                     /translation="MATQLVITALLIGLGAGMLSGLVGIGGGIIMVPALVFFMHYSQH
                     QAQGTSLAVLTLPVVILASFYYYSQCQKLGTPIDLRIVGLLATGFIVGAFFGSKIALQ
                     IHTDSLKKIFAVILFYTAIKMLNWDTALIKWIRSLAS"
     gene            1847..2470
                     /locus_tag="PK977_14885"
     CDS             1847..2470
                     /locus_tag="PK977_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19458.1"
                     /translation="MKTVITISFLFLPFCLNSQSVYNISKDYFRTDPFKNSFSNFVNQ
                     LINDPDVTEKKITKRSDSALFYFEGVYKSYSPFFIPVHYCKIILAEKEDRTNDSTQTP
                     FFYYEYQLLGYVSGKDEGLKDIKQEFEKLNKKFSRILTPEEKRELKRNSEVAGNIINY
                     SYKKMAFLPVTLAWASSPGSTDYLIAITLRFLNINNAAYLPIPADSP"
     gene            complement(2444..>3109)
                     /locus_tag="PK977_14890"
     CDS             complement(2444..>3109)
                     /locus_tag="PK977_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HRF19459.1"
                     /translation="VLKKSGNLWVKVRSLHDGYEGWMTHTLLMETDKRFANTRTACIT
                     TDILSSIVMGEKRINVPVGSSLPFFADGKGKLGSMEYSFSGHYFQRDEQQPGEELVRQ
                     LSSAWLNAPYLWGGRTPLGVDCSGFVQVIYKLMGIDLPRDAWQQAQEGEPVKKLIEAK
                     TGDLAFFDNKEDIVHVGIILGKDQLIHASGKVRIDELTKKGIQSDGPLPSGSRLRAIR
                     RYW"
     assembly_gap    3110..3233
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3444..3469
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3470..3930
                     /locus_tag="PK977_14895"
     CDS             <3470..3930
                     /locus_tag="PK977_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF19460.1"
                     /translation="LKNEDEAKDSVQQVFLKVIQELHKYKVEYFKSWLYMVAKNHCLM
                     RLREKQGKITAEINEKLTAQPEEETDRQLLVSNDRTLAIMEEALQELNPEQRQCVTLF
                     YLEKKSYQEVSEATGYSLLQVKSYIQNGKRNLKIMIEKRLSEKAIRQTGK"
     gene            3899..4294
                     /locus_tag="PK977_14900"
     CDS             3899..4294
                     /locus_tag="PK977_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19461.1"
                     /translation="MKKRSGKQENNMQENYKDILSNLSTDIDQETLLLYLQGKLSDEK
                     KHEVEKQLLNNDFDEDAVEGLQEFKDKEQLQYMVEMLNRDLKTRTEKKKKRREKMKIK
                     DQPWLYISIIILFILIAISYLVIKKMMQP"
     gene            complement(4291..4773)
                     /locus_tag="PK977_14905"
     CDS             complement(4291..4773)
                     /locus_tag="PK977_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRF19462.1"
                     /translation="MIQSERDNKFLQKAVELARQGMLSGNGGPFGCVVVHGDEIIGEG
                     SNMVLSTNDPTAHAEVVAIRQACEKLGTYQLDECEIYTSCEPCPMCLGAIYWARPRRV
                     VYANTREEAASIEFDDEFIYKEINTKMEERKIPFIHHPHQGAKELFDFWKTWPGKTMY
                     "
BASE COUNT         1552 a         1024 c          950 g         1399 t
ORIGIN      
        1 tgcaggaatg aaaggaaata atctattctt tttgttcctg cttttttttt gcagcctgca
       61 aaagtgacga caagaaacct gatgcaaaat catccaacag tattgatgcc gcccgaaatt
      121 tcataagagc tgcactggat ggcaaataca gagaagcgaa agactacctg ctgcaagatt
      181 ctgtcaacct gaactggatg gatatagcag aaagaaatta cctgaaagcc gaccaggaca
      241 caaaagatgg ctacagggcc tcctccatta atatgcatga agttacccct gttaatgatt
      301 ctactgccat tatcatttat tcgaactctt ttaaaaacga ccacgacaca ttaaaggtgg
      361 taaaactcaa cgggcagtgg ctggttgatc ttaaatatct ctttgagcat gacagagaca
      421 ctcttatcaa aaaacctgtt attaacgata cgataaagtg ataaagaaca ttcttctggt
      481 aggattagga ggaggtgcag gcagcatatt gcgctacttg tgccagcgtt ggttcgcaga
      541 agtatattct cattcttttc cctggggtac ttttgccgta aacattaccg gctgttttct
      601 tattggcgtc ttctggggtc ttaccttcaa aacttttgac agcaatgaac actggaaatt
      661 attcctgatg acaggtattt gcggtggctt tactaccttt tctgccttta cactagaagg
      721 tattggcctg ataagagaac aaaaacttgg gatgttcttt ctttatgttg ccggcagtgt
      781 ggtagcaggg ctggcactta cattagtagg catgaaatta acccgataat acaatgacaa
      841 cagttttaca tccatggcat ggcgtaaatc acggcgaaaa agcaccagcc accgtgaatg
      901 cactgattga aataccacag ggctcccggt cgaagtatga agtggacaaa gccacaggtc
      961 tgctgaaact ggacagggtc atttattcgt cgtttcaata ccccatcaat tatggcttca
     1021 ttcctcaaac actgggattg gacaatgacc cgcttgatat attggtaatc tgctctcaat
     1081 ccatccagtc tctttgtctt gtagaagcca atgtgatcgg caatatgcaa atgattgata
     1141 gtggacagga agatgacaaa atcatcgcag tcgcagcaaa agatccatca gtaaatcata
     1201 ttacaagaat ggaggaactg ccccagcatt ttttactgga actaaaaaac ttttttgaac
     1261 aatataaagt actagaaaat aaaaaagtag agatcgacac cttccaggac cagttaatcg
     1321 cctataagat tatcaacgac tcaatccatt tctacaaaga aacattcaaa aaataaccag
     1381 tgctcactcc tcaaaaaaat actatggcaa cacaattagt gatcacagct ttgctgatag
     1441 gcttaggtgc aggcatgtta agtggcctgg tcggaattgg cggtggcatc atcatggtac
     1501 cggcattggt attttttatg cactactcac aacaccaggc acaaggcaca tctttggcag
     1561 tactgacgct gcctgttgtt atattagctt ctttctatta ttactcacag tgccagaaac
     1621 tgggaacacc gatagacctt agaattgtag gtttattggc tacaggtttc attgtcgggg
     1681 ctttttttgg aagtaaaatc gccttgcaga ttcatacaga ttctttgaag aaaatatttg
     1741 cggtcatact tttttatact gctattaaaa tgctgaactg ggatactgca cttattaaat
     1801 ggatcagaag cctggccagt taattgttat taccggttcc tgtattatga aaactgttat
     1861 tactatatca ttcctgttct tgccgttttg tctgaacagc caatcagttt ataatatttc
     1921 taaagattat ttcaggaccg accctttcaa aaattctttt agcaatttcg tcaaccagtt
     1981 gattaatgat ccggatgtaa cggaaaaaaa gatcacaaaa agatcagact ctgctttgtt
     2041 ttattttgaa ggtgtttaca aatcctacag cccgtttttt atccctgttc attactgtaa
     2101 aattattttg gctgaaaaag aagaccgtac aaatgacagc actcaaaccc ccttttttta
     2161 ctatgaatac cagcttttag gctatgtgag cggcaaagat gaaggactaa aagatatcaa
     2221 gcaggagttt gaaaaattga acaaaaaatt cagtcgtatc ctcactccgg aagaaaagcg
     2281 ggaacttaaa agaaattccg aagtcgcagg caacatcata aattactctt acaagaaaat
     2341 ggcttttctt cccgttaccc tggcatgggc ttcttcccca ggatcaacag actatttaat
     2401 tgccattacc ctccgttttc tgaatattaa taatgctgct tatttaccaa taccggcgga
     2461 tagcccttaa ccggctgccc gatggaagcg ggccgtccga ctgaattcct ttttttgtaa
     2521 gttcatcaat cctcacttta ccggaagcat gaattaattg gtcttttccc agtatgatac
     2581 ctacatgaac tatatcttcc ttattgtcaa aaaatgccag gtcacctgtt tttgcttcaa
     2641 ttaacttttt taccggctca ccttcctgtg cctgttgcca ggcatccctt ggcaggtcaa
     2701 tacccatcag tttgtaaatc acttgcacaa acccgctgca atccactccc aacggtgtcc
     2761 ggcctcccca caggtacggt gcattgagcc aggcggaact caattgcctt actaactcct
     2821 ccccgggctg ttgttcatct ctttgaaaat aatgtcctga aaaactgtat tccatactac
     2881 ccaatttgcc tttaccatcc gcaaaaaagg gtaaagaaga acccactggt acattaatcc
     2941 ttttttcacc cataacaata ctgctcaata tatccgttgt tatgcaggca gttcttgtat
     3001 tggcaaatcg cttgtctgtt tccatcaaca aagtatgtgt catccagcct tcgtagccat
     3061 catgcagact tctgaccttt acccacagat tcccggattt tttaagaacn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatgtaa
     3241 aataaagcaa tatcctgtag caaaattttg cagaaattgt caaatcctgc atcttatcga
     3301 aaacaaatag ttattttccg cttgtgaagg cagatgatta taaagaatta acggaccagg
     3361 aactgcttga acagttttat gccgatcatg acaacgaatg gcttggcatc ctgctgcagc
     3421 ggtatacgct gcttcttttg ggtnnnnnnn nnnnnnnnnn nnnnnnnnna cctgaaaaat
     3481 gaggatgagg caaaagacag tgtgcaacag gtattcctga aagtgatcca ggaactgcac
     3541 aaatacaagg tagaatattt taaatcctgg ctttacatgg tagcaaaaaa tcattgcctc
     3601 atgcggctca gggaaaaaca aggaaagatc actgcagaaa taaatgaaaa acttacggct
     3661 cagccggaag aagagacaga caggcaactg ctggttagca atgaccgcac acttgctata
     3721 atggaagaag cgctgcaaga actaaacccg gagcaacgcc agtgtgtaac tttgttttac
     3781 cttgaaaaaa agagctacca ggaagtgagt gaggcaacag gatattctct gttacaggta
     3841 aaaagctata ttcaaaatgg caagaggaac cttaaaataa tgattgaaaa aagactgagt
     3901 gaaaaagcga tccggcaaac aggaaaataa tatgcaggaa aactataaag acatattatc
     3961 aaatttatcc accgatattg accaggaaac cctgttgttg tacctgcagg gaaaactatc
     4021 tgatgaaaaa aagcatgagg tggagaaaca attactcaac aatgattttg atgaggacgc
     4081 cgtggaaggg ctgcaggaat ttaaagataa agaacagctc cagtatatgg tagagatgct
     4141 gaaccgtgat cttaaaacaa gaactgaaaa gaaaaagaag cggcgggaaa aaatgaagat
     4201 aaaggatcag ccctggctgt atatttcaat tatcatcctc tttatattaa ttgcgatctc
     4261 atacctggta attaaaaaga tgatgcaacc ttagtacatg gtttttccgg gccatgtttt
     4321 ccaaaagtca aacaattctt tagctccctg gtggggatga tgaataaaag gaatctttct
     4381 ttcttccatt ttggtattga tctccttata aataaattca tcatcaaatt cgatactggc
     4441 tgcttcttct ctggtattgg catataccac ccgcctgggc ctggcccaat aaatagcacc
     4501 caggcacatc ggacatggtt cacaacttgt ataaatctca cattcatcca attggtatgt
     4561 tcccagcttc tcacaggctt gccttattgc tactacttca gcatgtgctg tagggtcatt
     4621 ggtagaaaga accatattag acccttctcc aattatttca tcgccatgaa ccacaacaca
     4681 gccaaagggg cctccgttgc cagacagcat tccctgccta gccagttcta cagctttttg
     4741 cagaaattta ttatctcttt ccgattgtat catataattc ggttaagtcg ttttcagtat
     4801 aaaattgtga ataacagggt gattttccac agattgttca ctaaaaaaga ttccaatatg
     4861 aagttaatat taattttttg ttacctgtct tgtgaagcga aaaagggatg taatttttaa
     4921 tcacaaacca acacctgatt atatgaaatg ggaaactgct gaaagttcaa accaacacga
     4981 agtttatcac ctgtataagg acgacaaaaa aatactaagc ctgacagtta ataaattctc
     5041 caattcggca agggttgatt gtaataaaga gaaaa
//
LOCUS       DAORLS010000230         5054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9054_length_5054_cov_1.995399, whole genome shotgun sequence.
ACCESSION   DAORLS010000230 DAORLS010000000
VERSION     DAORLS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9054_length_5054_cov_1.995399"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>251)
                     /locus_tag="PK977_14910"
     CDS             complement(<1..>251)
                     /locus_tag="PK977_14910"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRF19463.1"
                     /translation="AFINVTYHRSEHVFKKRADGTFEKVVVRKRPGTESICAAIMNKY
                     NVDTVPHLICGGFGVNETEDALINLHYLGIDNVLVLRGD"
     assembly_gap    252..428
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            577..1365
                     /gene="lptB"
                     /locus_tag="PK977_14915"
     CDS             577..1365
                     /gene="lptB"
                     /locus_tag="PK977_14915"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRF19464.1"
                     /translation="MSVTIRTENLVKKYGQRVVVNHVSFEVKQGEIVGLLGPNGAGKT
                     TSFYQVVGLVKPDEGKVFLNDQDITALPMYKRAQMGIGYLPQEASVFRKLSVEDNISA
                     ILEMTKLSKQQQRDKLESLLNEFRLQHVRKSNGDVLSGGERRRTEIARALSVDPKFIL
                     LDEPFAGIDPIAVEDIQAIVAKLKYKNIGILITDHNVTETLSICDRAYLLIEGKIYKH
                     GTAEELADDEEVRRLYLGRNFELKRKDYLHEEAIRASGAEEGDR"
     gene            1446..2933
                     /locus_tag="PK977_14920"
     CDS             1446..2933
                     /locus_tag="PK977_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF19465.1"
                     /translation="MRLWRIEAWRNNPLDAQREVLQDLVTSAQYTEFGRKYNFSNLFN
                     VRDFKATVPIHEYEDIKPYIERIMHGEQNILWNTPIYWFAKSSGTTSDKSKFIPISEE
                     SLQDGHYKASKDVLSLYYQYHPDSALLTGKGLVIGGSHNINPMNAEAQYGDLSAVLLQ
                     NSPFWGHWLRTPDLSIALMDEWESKIEKLAESTIKENVTSISGVPTWTLVLFKRILEI
                     TGKQTMSEVWPSLELYMHGGVSFTPYKEQFRKIIGKEINYLEMYNASEGFFAAQDIPG
                     SEGMLLFTDHGIFMEFMPVSEYGKKEPQTVSLQDVELGKNYALIISTNGGLWRYLVGD
                     TVQFVSLKPFRIKVSGRLKHYINAFGEEVIVDNTDKAIAIASNLTGAIVNDYTAAPVY
                     FSDSANGAHEWLIEFEKEPENLELFTKELDIALKSINSDYEAKRHKSIALGMPVIHAM
                     QKGAFTEWLRSKGKLGGQHKVPRLSNDRTMIDEILKMNLSTFTTQ"
     gene            2945..3697
                     /gene="fabG"
                     /locus_tag="PK977_14925"
     CDS             2945..3697
                     /gene="fabG"
                     /locus_tag="PK977_14925"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRF19466.1"
                     /translation="MLLKGKVAIVTGAARGIGEGIALKFAENGAHVAFTYVSDSSAEK
                     AAALETKLTGMGVKAKAYKSNAGDFAQCEAFVNDVLKEFGTVDICVNNAGISKDNLLL
                     RMSPEQWNDVIQVNLNSVFYMTKQVIRPMMKAKTGSIINMSSIIGEIGNAGQSSYAAS
                     KAGIIGFTKSVAKELGSRNIRCNAIAPGFVETDMTSYLKEGEAADKYRAGIPLGKFAS
                     AEDIANATLFLASDLSSYITGQTLSVCGGLNI"
     gene            complement(3700..>4182)
                     /locus_tag="PK977_14930"
     CDS             complement(3700..>4182)
                     /locus_tag="PK977_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRF19467.1"
                     /translation="QQVSLAAAYAAFKKLKEKIGYVTPQKILQLSDEEMRACYFTRQK
                     MGYARHLAEAIIKKQISLKKLSLLPDEEVREQLIRLKGIGHWTIDVYLMHALQRTDLF
                     PLGDIALVNSLKETKQLHPHVSKDEMLVIAEPWRPYRTIASMILWHSYIKKRNIKLQG
                     "
     assembly_gap    4183..4267
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4343..>5054)
                     /locus_tag="PK977_14935"
     CDS             complement(4343..>5054)
                     /locus_tag="PK977_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRF19468.1"
                     /translation="QKEFRNEIQQQDLAQAIHGAGNDEETKVIVLRSFGDKTFCSGAS
                     FDELVAIKNKEQGLEFFSGFAKVINAIRICPKFVIGRIQGKCVGGGVGIAAACDYAIA
                     TEAAEVKLSELAVGIGPFVVGPAVERKIGKSAFCTLAIDATMWRSADWAKRKGLFAEV
                     YPGIPELDEAIRRLVQVLSHSSPEAMAEMKKMFWKGAEDWDTLLVERAAISGKLVLSD
                     FTRHAIEKFKAPKSHKGA"
BASE COUNT         1386 a         1074 c         1061 g         1271 t
ORIGIN      
        1 atccccccgc aataccaata cattgtcaat accgaggtaa tgaaggttaa ttaaggcatc
       61 ctctgtttca ttgacgccaa agccaccgca aataagatgg ggaacagtat ccacattgta
      121 cttattcata atagcagcac agatgctttc agtgccgggc cttttccgta ccactacttt
      181 ttcaaaagtt ccatccgctc tttttttgaa tacatgttcg ctccggtgat atgttacatt
      241 aatgaacgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnngg aatgccggat atgtactact atacagaatc tccttacaag catttcataa
      481 gcctgcaaag ctaacaatgc aggggcatta gtcaaaacga cgttaaatag agcagaaaac
      541 cttttaatcg acaaccatca cttatttttg ctgcccatgt cagttactat ccgcacagag
      601 aaccttgtaa aaaagtatgg tcaacgggtt gttgtcaacc atgtttcatt tgaagtgaag
      661 cagggcgaaa tcgttggttt gcttggaccg aatggtgcgg gtaaaaccac ttctttttac
      721 caggtagtgg ggctggtaaa gcccgatgaa ggcaaagtgt ttttaaatga ccaggatatc
      781 acagctctgc ccatgtacaa acgagcccaa atgggtatcg gttacctgcc gcaggaagct
      841 tctgtcttcc gcaagctgag tgtggaggac aatatttccg ctatcctgga aatgactaaa
      901 ctttccaaac agcaacaaag agataaactg gaatcattac ttaatgaatt tcgcctgcag
      961 catgttcgca aaagcaacgg tgatgtatta agtggtggcg aaagaaggcg tacggaaatt
     1021 gcccgggcac tgtcagttga tccaaaattt atcctgctgg atgaaccttt tgccggtatt
     1081 gacccgattg cagtggagga tattcaggcc attgtggcca aactgaagta taagaatatt
     1141 ggcatactca tcaccgatca caacgtaaca gaaaccttat ctatctgtga ccgggcctat
     1201 ttgctgatcg aaggaaagat atataaacat ggtacagccg aggaactggc cgatgatgaa
     1261 gaagtaagac gtctttacct gggaaggaac tttgaattaa aaaggaagga ctacctccac
     1321 gaggaagcta tccgggcttc aggagccgaa gaaggcgatc gttaattttc acccattcct
     1381 ttaatttctc tattttgccg gcagcaatga acctgttaag cccagccata tcatcattgg
     1441 cccgtatgcg cctctggcgc atagaagcct ggagaaataa tcccctggat gcacaaaggg
     1501 aggtattgca ggacctggtg acatcggccc aatacaccga gtttggccgg aaatataatt
     1561 tttccaacct gtttaatgtc cgtgatttta aagctacagt tcctatacat gagtatgaag
     1621 atataaagcc gtatatcgaa cggatcatgc acggggaaca gaatatttta tggaacacac
     1681 cgatctattg gtttgccaaa agcagcggca ccaccagcga taaaagcaaa ttcattccta
     1741 tcagcgaaga aagcctgcag gatggtcatt acaaagcatc caaagatgta ttgtcactat
     1801 attaccaata ccatcctgat tcagcactgc taaccgggaa aggtttggtg atcggcggca
     1861 gtcacaatat caacccaatg aatgctgagg cacagtatgg cgatctgagt gccgtactgt
     1921 tacaaaattc acctttctgg ggtcattggt taagaactcc cgacctgagc attgccctga
     1981 tggatgagtg ggaaagcaaa attgaaaaac tggcggaaag cactattaaa gaaaatgtca
     2041 cttccatttc cggggtgcct acctggaccc tggtactttt caaacgaata ttggagatca
     2101 ccgggaaaca aactatgagt gaagtgtggc cttcactgga actatatatg catggaggcg
     2161 tatcttttac tccttataaa gagcagttta gaaagatcat cggtaaggaa atcaactacc
     2221 tggaaatgta caatgccagc gaaggttttt ttgcagcaca agatataccc ggcagcgaag
     2281 gcatgctttt gttcaccgat catggcatct ttatggagtt tatgccggta agtgagtacg
     2341 ggaaaaaaga accgcaaaca gtaagtctcc aggatgtaga actggggaaa aactatgccc
     2401 tgatcatcag taccaatggt ggtttgtgga gatatcttgt tggcgataca gttcaattcg
     2461 tttccctgaa accattccgt atcaaagtat ccggcaggct gaagcattat atcaacgctt
     2521 ttggggaaga agtgatcgtt gacaatacag ataaagccat cgccatagcc agcaatttaa
     2581 ccggtgccat agtcaatgat tataccgcag cgcctgttta tttttctgac tcagccaatg
     2641 gcgcccatga atggcttatt gagtttgaaa aagaaccgga aaacctggag ctattcacga
     2701 aagaactgga tatagcactg aagagcatca acagcgatta cgaagccaag cgccataaaa
     2761 gtattgcatt gggtatgcct gtcattcatg ctatgcagaa aggtgctttt accgaatggt
     2821 tgcgaagcaa gggaaaactc ggcggtcagc acaaagtacc acgactatca aatgacagaa
     2881 ccatgattga tgagatactg aaaatgaatc tttctacatt taccactcaa taaactaaag
     2941 aaccatgtta ttaaaaggaa aagttgccat cgttaccgga gctgcccgcg gcattggaga
     3001 aggaatagca ttaaaattcg ctgaaaacgg agctcatgtg gctttcacct atgtaagcga
     3061 cagtagtgct gaaaaagcgg ccgcattgga aacaaaactg actggtatgg gtgtgaaggc
     3121 aaaagcctat aaaagcaatg ctggtgactt tgcccaatgt gaagcctttg taaatgatgt
     3181 gctgaaagaa tttggtacag tagatatatg tgtaaataac gcaggtatat ccaaggataa
     3241 cctactgctg agaatgagcc ccgagcaatg gaacgatgtg atacaggtaa acctgaacag
     3301 cgttttttat atgaccaaac aggttatccg ccccatgatg aaagccaaaa ctggttccat
     3361 catcaatatg agctccatta ttggtgaaat tgggaacgcc gggcaaagca gctatgcagc
     3421 cagcaaggct ggtattatcg gctttactaa atcagtagca aaagaactgg gcagccgtaa
     3481 tattcgctgc aatgccattg cccccggttt tgtagaaacc gatatgacat cctatttaaa
     3541 agaaggcgag gcggctgata aatacagagc tggcattcca ttgggcaaat ttgcatctgc
     3601 tgaagatatt gccaatgcta cactcttcct ggcttctgac ctgtcttcct atatcacagg
     3661 gcaaacgctg agtgtttgtg gcgggctaaa tatttaaagt cacccctgaa gcttgatgtt
     3721 gcgtttctta atgtaactat gccacaaaat catgctggca atcgtccggt agggtcgcca
     3781 gggctcggct attaccagca tttcatcttt ggagacatgc ggatgaagtt gtttggtttc
     3841 tttcaggctg tttactaagg ctatatctcc taaaggaaat aaatcggttc gctgcaaagc
     3901 atgcattaaa tacacatcga tggtccagtg gccaatgcct tttagccgga tcagctgttc
     3961 tcttacttct tcatccggta agagagacaa tttctttaag ctgatttgtt ttttgattat
     4021 tgcttcagcc aggtggcggg catatcccat tttttgccgg gtaaaatagc aagccctcat
     4081 ctcttcatct gaaagttgca gaatcttctg aggagttaca tacccgatct tttcttttaa
     4141 tttcttaaag gcagcgtaag cagccgccag cgaaacctgt tgnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnngag ctccttatcc tttttggtta gcccattaca aaaccggtga aagttgcttt
     4321 catcaaattg ctgatacatg cattaagccc ctttatgtga tttgggagct ttgaactttt
     4381 cgatggcatg ccgggtaaaa tcactcaata ccagtttgcc acttattgca gccctttcaa
     4441 ccaacaaggt atcccagtct tcggctcctt tccagaacat ctttttcatt tcagccatcg
     4501 cctccgggct tgaatgagac aatacttgta ctaatctcct tatggcttca tccagttcgg
     4561 gtatgccggg gtatacttct gcaaacaaac cctttcgctt ggcccagtcg gcgctgcgcc
     4621 acatggtagc atcgatagcc aacgtacaaa aggcagactt gccgattttt ctttccaccg
     4681 ccggccccac cacaaaagga ccaataccca ctgccagctc actcaatttc acctcggctg
     4741 cttcagttgc aatggcataa tcacaggcgg ctgcaatgcc tacaccgcca cctacacact
     4801 taccctgtat gcggccgatt acaaacttgg gacagatacg gattgcattg attactttgg
     4861 caaagccgct gaaaaattca agtccctgtt ctttgttttt aatagcaacc agctcgtcaa
     4921 aactggcccc ggaacaaaac gttttatctc caaaactgcg taacacaatc actttggttt
     4981 cttcatcatt gccggctccg tgtatggcct gggccaggtc ttgttgttga atttcattgc
     5041 ggaattcctt ttgc
//
LOCUS       DAORLS010000231         5018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9149_length_5018_cov_1.412251, whole genome shotgun sequence.
ACCESSION   DAORLS010000231 DAORLS010000000
VERSION     DAORLS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9149_length_5018_cov_1.412251"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PK977_14940"
     CDS             <1..752
                     /locus_tag="PK977_14940"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRF19469.1"
                     /translation="NLGARQNVLTDENGKNHGFKPGFAYAAGIEPLLAMPVKGILCYQ
                     GESNAQEAERVNEYAMLSKLMVDDYRVKWNDPKLPFYFVQLSSIDTAKYKGHLWPEFR
                     DEQRKMLQLIEYSGIAVSSDIGARHDVHPTNKKSVGERLSKWALCKTYKKKLLPSGPM
                     PVRAVYRKKRVLIRFRFTGKKLITMYGQLLRGFSLNGKDDAIASIQRRHVEIRFDRKP
                     EYVYYGWKPFSDGNLFNSENLPASTFKIRVK"
     gene            749..>1065
                     /locus_tag="PK977_14945"
     CDS             749..>1065
                     /locus_tag="PK977_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRF19470.1"
                     /translation="MKRVITILSLLHSAFLSAQVKLPVYPDSNFSAYYHQRHTLFQSL
                     PQTKNDIIFIGNSITDGNEWSEMFGDLRIKNRGISGDITAGVVKRLDEVVKRKPASVF
                     LMIG"
     assembly_gap    1066..1242
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1254..>2074
                     /locus_tag="PK977_14950"
     CDS             1254..>2074
                     /locus_tag="PK977_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF19471.1"
                     /translation="MNKRLFDSASALQYAFIDLYTSFCDKQGKLEAGLTNDGLHLSGA
                     GYILWKHLVFPHVYGLQEKPSLIPLPQQLKWKSGFFSLLGCRFIVVKGDSLENEAGIL
                     KKAIEAFGWDVDIKTVIPGSERHIELRIEKSGVFSSNKEAYGLKVSTDKILITATTSH
                     GIFNGIQTLRHLMRDGVMVDACDITDWPAFSWRGYMIDVGRNYMSMKLLKQQIDVMAA
                     NKLNIFHFHATEDIAWRIASKQFPQLNAPEHMLRNKGMYYNEAEIKELIAYCKER"
     assembly_gap    2075..2188
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2189..>3040
                     /locus_tag="PK977_14955"
     CDS             <2189..>3040
                     /locus_tag="PK977_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRF19472.1"
                     /translation="KEFCTTYDVPYIHIGADEVKIVNKQFIPEVTSYIEGFGRKVIGW
                     QPGGNFSNSTIRQLWMDDNAHHTAGSEIQFIDSRHLYLNHMDPLEAVTTIFNRKIADK
                     EKGDESTLGGTICMWHDRAVSKEDDILNMNPVYPGMLAFAERSWKGGGVSGWVANISN
                     GDEDAFAEFESRLLDNKQLYFKDKPFGYVKQSGMQWKLYGPFDNGGDVHKKFFPEIQS
                     AEPVKKQPYKQVTGGTIVLRHWWAPLIKGAIEEPKENTTWYDSTNIWMDGAGERECWI
                     GFNNLSRS"
     assembly_gap    3041..3078
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3079..3333
                     /locus_tag="PK977_14960"
     CDS             <3079..3333
                     /locus_tag="PK977_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRF19473.1"
                     /translation="GAWDEKGSTIWVNGNLMPPPVWLRGGQRGNAEVPLMDEGYEYRE
                     PVKIYFQNGWNTVLIKAPVTTFKGKNWQNPVKWMFTFVLK"
     gene            3360..>3953
                     /locus_tag="PK977_14965"
     CDS             3360..>3953
                     /locus_tag="PK977_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF19474.1"
                     /translation="MQFFVSLVLLVFFTGNYSVIAQKNKSALPNIIYIYADDMGYGEL
                     GCYGQQKIKTPNIDKLAEEGIIFTQHYTSSPVCAPARCMLLTGMHGGHSYIRGNYELG
                     EFSDENEGGQMPLPPGVFTLPMMLKKAGYSTALCGKWGLGMPAAGGSPLMHGFDYYYG
                     FLDQKQAHNYYPTHLWENDKWDTLDNHAMNVHQRKDSS"
     assembly_gap    3954..4071
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4072..>4659
                     /locus_tag="PK977_14970"
     CDS             <4072..>4659
                     /locus_tag="PK977_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF19475.1"
                     /translation="PYTIPHVSLQAPEEYVKKYIGLFDEKPYYGQKGYAANKFPFSTY
                     AAMITYLDDQVGLIMKRIKELGIDNNTFIMFSSDNGASAAGGANPDFFNSVRGLRGLK
                     MDLFEGGIRMPFIARWPGKIRAGSQTQHISAQYDVMATFAELTKQQLTTTDGISFLPE
                     LLGKATIQKKHPYLYFEYPENGGQVAIRMGKWKAI"
     assembly_gap    4660..4802
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1367 a          743 c         1045 g         1273 t
ORIGIN      
        1 aaaatttggg tgcaaggcag aatgttctta ctgatgaaaa tggtaaaaac catgggttta
       61 aaccgggctt tgcttatgcc gcaggtattg agccactact tgcaatgccg gtgaaaggta
      121 ttctttgtta ccagggagaa agtaatgcac aggaagctga aagagtaaat gagtatgcga
      181 tgttgtcgaa gctgatggtg gatgattacc gggtaaaatg gaatgatcca aaattgccct
      241 tttattttgt ccagttatct tcaattgata ctgcaaaata caaaggccat ttatggccag
      301 aattcaggga tgagcaaagg aaaatgttac agctgattga gtatagtggt atagctgtaa
      361 gtagtgatat tggagccaga catgatgtgc atcccacaaa taaaaagtca gttggtgaaa
      421 gactgtcaaa gtgggctcta tgtaaaacct ataaaaaaaa attattgcca tcagggccta
      481 tgcctgtccg ggcagtgtat agaaaaaaaa gagtactgat tagatttcgt tttactggaa
      541 aaaaactaat cacaatgtac gggcaattac taagaggatt ctctttgaat ggtaaagatg
      601 atgcgattgc ttctattcag cgaagacatg tagagattcg gtttgacagg aagcctgagt
      661 atgtttatta tgggtggaaa cctttcagcg atgggaatct ctttaattcg gaaaatctcc
      721 cggcctcaac atttaaaatc agggtgaagt gaaaagagtt atcacaattt tatccttatt
      781 gcattcagcg tttctatctg cacaagtgaa actgccggtt tatccggatt caaatttttc
      841 tgcttattat catcagcgac acaccttatt tcaatccttg cctcaaacaa aaaacgatat
      901 aatttttatt ggtaatagta ttacggatgg taatgaatgg agtgaaatgt ttggagattt
      961 aagaatcaag aatcgtggta taagtggtga tatcactgct ggtgtggtga agaggttgga
     1021 tgaagtagtg aaaagaaaac ctgccagtgt ttttctgatg attggnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaataaaa gaagtgaata
     1261 aacggctttt tgacagtgca tctgcccttc aatatgcgtt tatcgatctg tacacttcct
     1321 tttgtgataa acaaggaaaa ctcgaagccg gcttgacaaa tgatggcctg catctctccg
     1381 gtgcaggata cattctttgg aaacatctgg tattcccgca tgtttatggt ttgcaggaaa
     1441 agccctcgtt gattccacta ccacaacaat taaaatggaa gtcaggcttt ttctctttgc
     1501 ttggctgcag atttatagtt gttaagggtg acagcctgga aaatgaggct ggtattttga
     1561 agaaagcaat cgaagctttt ggatgggatg tggatattaa aacagtgata cctggttcag
     1621 aaagacatat tgaattgcgt atagaaaagt ccggtgtttt ttcctctaac aaagaagcgt
     1681 acggtttaaa agtttcaaca gataagatcc tgataactgc aactacatct catggaatat
     1741 ttaatggaat tcagactttg cgccatctga tgcgtgatgg tgttatggtg gatgcctgcg
     1801 atataacaga ttggccagct ttttcctgga gaggttacat gatagatgtt ggaagaaatt
     1861 atatgtcgat gaaattgctg aagcagcaga ttgatgtaat ggctgccaat aagctcaata
     1921 tctttcattt tcacgcaaca gaagatatag cctggagaat tgccagcaaa caatttccac
     1981 aacttaatgc acccgagcat atgctccgaa ataaagggat gtattataat gaggcagaaa
     2041 tcaaagagtt gattgcctat tgtaaggagc gacannnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aagagttctg tacaacctat gatgtgcctt
     2221 acatccacat cggggcagat gaagtaaaga ttgtcaataa acaattcata cctgaggtaa
     2281 cttcatatat tgaaggtttt ggtagaaaag tgattggatg gcaaccaggt ggtaattttt
     2341 ccaacagtac tatccgccag ttgtggatgg atgacaatgc acatcatact gctggtagtg
     2401 aaatacaatt tattgattca cggcatttgt atctgaatca tatggatcca ttggaggcag
     2461 ttactaccat tttcaacaga aaaattgcgg ataaggaaaa gggtgatgaa tcaacgctgg
     2521 gtggtacgat atgtatgtgg catgacaggg cggtgagcaa ggaagatgat attctcaata
     2581 tgaatcctgt ttatcctggc atgttggctt ttgcagaaag aagctggaag ggaggaggag
     2641 tctccggatg ggtagctaac attagcaacg gcgatgagga tgcttttgct gaatttgaaa
     2701 gcaggttact ggataataag cagctgtatt ttaaagataa acccttcggg tatgtaaaac
     2761 aatcgggtat gcaatggaaa ttgtatggtc cgtttgacaa tggaggcgat gtccataaaa
     2821 aattctttcc tgaaattcag tcagcagaac ctgttaagaa gcaaccgtat aaacaagtta
     2881 cgggaggtac tatcgttctg cggcattggt gggcgccttt gattaaagga gcgattgaag
     2941 agcccaaaga aaatacgaca tggtatgact ctacgaatat ttggatggat ggtgcaggtg
     3001 aaagagaatg ttggattggg tttaataatt tatctcgttc nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnngg tgcgtgggat gaaaagggaa gtacgatctg ggtaaatgga
     3121 aacttaatgc ctcctcctgt ttggctgcga ggtggtcaaa gaggaaatgc tgaagtgcca
     3181 ttgatggacg aagggtatga ataccgggaa cctgtaaaaa tttattttca aaatggctgg
     3241 aatactgtat tgattaaagc gccggtcact actttcaaag gaaagaactg gcaaaaccct
     3301 gtaaaatgga tgtttacgtt tgtattaaaa taaataatct gaactaaacg gtatcaatca
     3361 tgcagttttt tgtctcatta gtcttattag tcttttttac aggcaattac tctgtaattg
     3421 cgcaaaaaaa taaatctgca ttgcctaata tcatttatat atatgcagat gatatgggct
     3481 acggtgagtt aggttgttac gggcagcaaa aaattaagac accgaatatt gacaagctgg
     3541 cagaagaggg cataattttt acacaacact acaccagttc acctgtatgt gcgccggcac
     3601 gctgtatgct tcttacagga atgcacggcg gtcactcata tatacgaggt aattatgagc
     3661 ttggcgagtt ttcagatgaa aatgaaggag gtcagatgcc tcttcctccg ggagttttta
     3721 cactacccat gatgctgaag aaagcaggtt acagcacagc cttgtgtggc aagtggggtc
     3781 ttggaatgcc tgctgcagga gggtcgcctt taatgcacgg gtttgactat tattatggtt
     3841 ttctggatca gaagcaagcc cataattact atccaactca tttgtgggaa aacgacaaat
     3901 gggatacgtt ggataatcat gctatgaatg tgcaccagcg gaaagattct tcannnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntccttatac
     4081 aataccacat gtttccttgc aggccccgga ggagtacgta aaaaagtata ttggtttatt
     4141 tgatgagaag ccatattatg gtcaaaaggg atacgcagcc aataaatttc ctttttccac
     4201 ttatgcagca atgattactt atctcgatga ccaggtggga ttgatcatga aaaggatcaa
     4261 agaacttggc attgacaata atacctttat aatgttcagc agtgataacg gggcttctgc
     4321 tgctggcggc gcaaaccctg atttttttaa tagtgtcaga gggctccgcg gcttgaaaat
     4381 ggatttgttc gaagggggca ttcgtatgcc atttatagca cggtggcccg gcaagattcg
     4441 ggcaggtagt caaacacagc acatctctgc tcagtatgat gtaatggcaa cctttgcaga
     4501 gttgacaaag cagcaactta caactactga tggtatttct tttttgccag agttgcttgg
     4561 aaaagccact attcaaaaaa agcatcctta cctgtatttt gaatatccgg aaaatggcgg
     4621 acaggtagct atccggatgg gtaaatggaa ggctataaan nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nngcccgcat attaaagaat gggagatcat tgaccccagg tttgataaat aaatctcttt
     4861 ttatatcaat ataaacgaat aataaatgaa gaagatcctt ttagtctcac ttttatttgt
     4921 cttatcggct gctttgcagg cgcagacgta tcatcccaca gcggagaacc ttgctgcccg
     4981 aaaagcattt caggatagga aatttggact ttttattc
//
LOCUS       DAORLS010000232         4924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9398_length_4924_cov_1.690902, whole genome shotgun sequence.
ACCESSION   DAORLS010000232 DAORLS010000000
VERSION     DAORLS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9398_length_4924_cov_1.690902"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..>391
                     /locus_tag="PK977_14975"
     CDS             56..>391
                     /locus_tag="PK977_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase group 1"
                     /protein_id="HRF19476.1"
                     /translation="MKKADGIFFITPEDKSFAINTWKIEPTRCFDVPFGVEIEKYPEN
                     RLQYRNQIAGKHNIPVEEKILLFNGLLNYKPNRDALKVILENINPLLIKKEGFKYKIV
                     ICGKGLPDEM"
     assembly_gap    392..540
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <541..750
                     /locus_tag="PK977_14980"
     CDS             <541..750
                     /locus_tag="PK977_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19477.1"
                     /translation="FGATVIATKTGAAGIEKSVCGKKLTIVDDNDWETFVNRIMYTET
                     KEATPAEFYSLYNWDQIIKKLKLSL"
     assembly_gap    834..878
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <879..1736
                     /locus_tag="PK977_14985"
     CDS             <879..1736
                     /locus_tag="PK977_14985"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRF19478.1"
                     /translation="EQLLQSPPTANAGPNQTVQLPQASITLTGSGTTTNGTITAYLWS
                     LVSGPNVPVVTSPGSPTTTITGVIAGTYIFQLMVVDNAGLTGVDTTKIICSQAPIQTL
                     SLQPANNTNEVHFAVLGGVSQTDPTAPELVAASWTSGGAPYDFRGALKFDLSSIPASA
                     TILTASLTLYSNPTPLNGNHVDANFGTNNAMYIRRLLSNWTASTITWNNQPASSVSDQ
                     VLIPHTSASFLDLTNIDVKNQVAAMIATGNHGFIIGLQNETPYNSRIFCSSKYSNTGK
                     HPKLVITYQ"
     gene            complement(1730..>2199)
                     /locus_tag="PK977_14990"
     CDS             complement(1730..>2199)
                     /locus_tag="PK977_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF19479.1"
                     /translation="FFKKETASRFKLVIAGSEMYRSGELIDYKNTMTFGQDVIFTGRV
                     NDQVLNDIYGAAHAFVFVPFFEGFGIPLIEAMQCHVPVIASNVTSLPEVAGEAAIFVD
                     PANINEIKNAMIRIYNNDDLRNDLIKRGDIQKKLFSWERTADLLWEGINKCLY"
     assembly_gap    2200..2252
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2253..2867)
                     /locus_tag="PK977_14995"
     CDS             complement(<2253..2867)
                     /locus_tag="PK977_14995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19480.1"
                     /translation="MKIAVNTRLLRKGKLDGIGWFAFNTLQYITRKNPEVEFHFFFDS
                     GIEKEFLFGHNVIPHNLFPPAKHAALNILWSEVSVKAKLNSIKPDLYFSPDGMLCLGW
                     QGRQYAVIHDINFMHYPEFLKYSNRKYYRYYFPKFAKKASRIATVSSYSKEDIVNTFG
                     IEEDKIDVVYCGINSFFKPLSSDEISAIRNKYTSGELYFLFVGAL"
     gene            complement(2864..3814)
                     /locus_tag="PK977_15000"
     CDS             complement(2864..3814)
                     /locus_tag="PK977_15000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19481.1"
                     /translation="MDRPFISICIPAYKNEEFLSRLLDSIRIQDYRDFEVIISDDSPD
                     FRLKELVETYRKYFTIFYIKNEKPLGSPVNWNNAISHAKGKWIKLMHDDDWFVDSSSL
                     HKFAYQAINGQSSFIFSGFYNVKASDKSMSKQQIGFVDRILLKKNPIYLLRKNFVGHP
                     STTIIKNENIKWYDERYKWVVDIEFYIRSFRRDNRYTIIKTPLVNLGISDQQITSAVF
                     RRPEVEIPENISLLNDISPGSLKNIFAYDYYWRFIRNLSIRSTEGIHKYYDSIAVPPI
                     LKFMIKQQARIPFNLTRIGMVSKITMLFTYLWNRFKGRIK"
     gene            complement(4447..4707)
                     /locus_tag="PK977_15005"
     CDS             complement(4447..4707)
                     /locus_tag="PK977_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19482.1"
                     /translation="MISIIICSISPEFLGQVKENISDTIGLEYELLVWDNRQANIGLC
                     EVYNRMAGQAKFEFICFLHEDIYFERTGWGIDLISYSTAEMS"
BASE COUNT         1559 a          941 c          730 g         1447 t
ORIGIN      
        1 caaaagtaca ggccgctggt ggtggcccat tctaaaatgg tatgaaaaaa gctgtttgaa
       61 aaaagcagat ggaatttttt ttattactcc cgaagacaaa tcgttcgcca ttaatacctg
      121 gaaaattgaa cccacaaggt gcttcgatgt tcctttcggt gttgaaatag agaaatatcc
      181 tgaaaacaga ttgcaatacc ggaatcaaat agctggaaaa cataatattc ccgttgaaga
      241 aaaaatactt ttgtttaatg gtttattaaa ttataaaccc aatagggatg cgctaaaagt
      301 tatccttgaa aacataaacc cacttctgat aaaaaaagaa ggtttcaaat acaagattgt
      361 catctgtggc aaaggcttgc cggatgaaat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 tttggtgcta ctgtcatagc cacaaaaacc ggggcagccg gaatagaaaa gtcggtttgt
      601 ggtaagaaac ttaccattgt agacgacaat gattgggaga catttgtaaa ccggatcatg
      661 tatacagaaa caaaagaagc cacaccagcg gagttttatt cactctataa ttgggatcag
      721 atcataaaaa aattgaagct cagtctataa ttagagtaaa aaaaagtaac ttactgttca
      781 atatttacaa caaactaatg aagccttatc taaaaagtac cctggcagct gttnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gcagctgtta caatcaccac
      901 ctacagcaaa tgcaggacct aatcaaacag ttcaactacc acaagcttcc ataaccctta
      961 ccggctcagg cactacaacg aacggaacca ttactgctta tttgtggagt cttgtttcag
     1021 gtccaaatgt tcctgtagtg acctcaccgg gttcacccac tacaaccata acaggagtta
     1081 ttgccggtac ttacattttt caattaatgg tagtagataa tgccggattg actggagtgg
     1141 atacaaccaa aatcatttgt tcacaggccc ctatacaaac actttcttta cagccagcga
     1201 ataatacaaa tgaagttcat tttgctgttt tgggaggagt gtcacaaaca gatcctacag
     1261 ctccggaact agtggctgca tcgtggacct ccggaggtgc accttatgat ttcagaggtg
     1321 ctttaaaatt tgatttaagc tcaataccgg cttcagcaac cattttaact gccagtctta
     1381 cactttattc taacccaact cctttaaacg gaaaccatgt tgatgctaat tttgggacaa
     1441 ataatgcaat gtatatacgc aggctgttgt ccaactggac tgcttctaca attacctgga
     1501 ataaccaacc agcctcttct gtttcggacc aggtgttaat accacatact tcggcctcat
     1561 ttcttgacct gacgaatata gatgttaaaa accaggtagc ggctatgatt gctacaggaa
     1621 atcatggctt cataatcggc ctgcaaaatg aaacacctta taactccagg atattttgtt
     1681 caagtaaata ttcgaatacg ggcaagcatc caaaattagt aatcacctat cagtaaaggc
     1741 acttatttat gccttcccat aaaagatcag cagttctctc ccatgaaaac agcttttttt
     1801 gtatatcacc ccttttaatc agatcattgc gcaaatcatc attattataa atcctgatca
     1861 ttgcgttttt aatttcatta atgtttgcag gatctacaaa tatagctgct tcaccagcaa
     1921 cttcaggtaa agatgttaca tttgaagcaa ttacaggcac atgacattgc attgcttcaa
     1981 ttaaagggat tccaaatcct tcaaaaaaag gaacaaatac aaatgcatgt gctgcgccat
     2041 aaatatcatt aagcacctgg tcatttactc ttcctgtgaa aataacatcc tggccaaacg
     2101 tcatagtatt tttatagtca atcaactctc ctgatcgata catttcactc cctgctatta
     2161 ctaatttgaa cctggaagca gtttcctttt taaaaaattn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaaagcac caacaaaaag aaaatagagt
     2281 tctccagaag tatatttatt ccgaatagca gaaatttcat cactactcaa tggcttaaaa
     2341 aatgaattta ttccgcagta aactacatct attttgtcct cttcaatacc aaatgtattt
     2401 acaatatctt ctttgctgta agaagaaacg gtagctattc tactggcttt ttttgcaaat
     2461 tttggaaaat aataccggta atatttccgg ttcgaatatt taagaaactc tggatagtgc
     2521 ataaagttta tatcatgaat aactgcatat tgcctccctt gccatccaag gcacagcata
     2581 ccatccggtg aaaaatataa atctggtttg attgaattaa gtttggcctt tacacttacc
     2641 tcagaccata gaatatttag tgcagcatgt tttgcgggcg gaaaaagatt atgaggaata
     2701 acattgtggc caaatagaaa ttctttttca attcctgaat caaagaaaaa atgaaattca
     2761 acttccggat ttttccttgt tatatattgc aaagtgttaa aggcaaacca gcctatgccg
     2821 tccaatttcc ctttacggag taatctggta tttacggcaa ttttcatttt attctccctt
     2881 taaatctatt ccacaaatag gtaaataaca ttgttatttt tgacaccatg ccaatacggg
     2941 ttagattaaa tggtattctt gcctgttgct ttatcatgaa tttaagtatg ggtggaactg
     3001 caatactatc ataatactta tgaattccct ctgtacttcg tatggataaa tttcttataa
     3061 aacgccagta ataatcataa gcgaaaatat tttttaaaga gcctggcgaa atatcattca
     3121 acaaactaat attctccgga atctctactt ctggtcttct gaatacagct gaggtaattt
     3181 gctggtcgct gatacccaga tttaccaatg gggttttaat aatggtataa cggttatctc
     3241 ttctaaaact ccggatataa aactcgatat ccactaccca cttatatctt tcatcgtacc
     3301 atttgatatt ttcatttttt attattgtcg tacttggatg accgacaaaa ttcttacgca
     3361 ataaataaat cgggtttttc ttcaataata ttctatcaac aaaaccaatc tgttgtttac
     3421 tcattgactt atcactggct tttacgttat aaaagccact aaaaataaaa gatgattgtc
     3481 cattgatagc ctgataagca aacttatgga gactactgct gtcaacaaac cagtcatcat
     3541 catgcatcag ctttatccat ttaccttttg catgtgaaat ggcattattc caatttaccg
     3601 gcgagcctaa tggtttttca ttttttatat aaaagattgt aaaatacttt ctataagttt
     3661 cgaccagctc ttttaatcta aagtccgggc tgtcatccga tataatcacc tcgaaatccc
     3721 tgtaatcctg gattcggatt gagtcaagca acctgctaag aaattcctca ttcttatagg
     3781 caggaataca aattgagatg aatggcctgt ccacttatgt tcctctttta tgaatataac
     3841 gcaaacctaa tggtttgtaa aataagaact tgaatattga ataaagcaac ttaaaattag
     3901 aatacagatt ttcattgaaa atccatctta gcttgttttt aaaagaaata tgctggcttt
     3961 tcagtacatc aagccatatt ttcttgatct tgaattctgc tttttctgac actttaatgt
     4021 caggcaaaat taccgggagc ctgttcaaaa aatatttatg aaaatgaaat gttgtgacaa
     4081 cccacttatc gccaaaatcc ccgccattag taacatgaac aagtttaaga ttatataata
     4141 cactgacact acaaatttga gatgccctca gactgaaatc taaatcgtag taatgaaatc
     4201 ctttgaggtc attatcatta aatcttattt tctgccaaat ttcctttcga caacaaataa
     4261 ataccccatc aatacaaact acatcttcat agtgtgtatt atgatctata tctggcttaa
     4321 gatagatatg ccttaccttc cctctttcaa tatgaactaa atccgcacaa tcaaaatccc
     4381 caatgcctgt gtaccatccg gagtaagctg cagatttata tttacttcct gcaacaccta
     4441 ttacccctaa ctcatttctg ctgttgaata agaaattaag tcaatgcccc atccagttcg
     4501 ctcaaaataa atatcctcat ggagaaaaca aatgaactcg aattttgcct gcccagccat
     4561 ccggttatat acttcgcaaa gcccgatatt tgcctgccgg ttatcccaaa caagtaattc
     4621 atattccagc ccgatggtat ctgaaatatt ttcctttact tgcccaagaa attcagggct
     4681 aattgaacaa attattatag atatcatcga attaaaaggg ctaatatact tgtaaaatcg
     4741 gtaaaaccgg aaatcatgaa taaaggatca ttctttaaaa atataaaaac cgggattcgt
     4801 cacagaatca ataaatcttt aaaaaatccg taccaaaaag caaaccttaa ctattttaaa
     4861 gtaaaatatc taaaacatct gcccccggga aaattaaatt ctcatattct ttatggagca
     4921 agca
//
LOCUS       DAORLS010000233         4833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9613_length_4833_cov_1.836961, whole genome shotgun sequence.
ACCESSION   DAORLS010000233 DAORLS010000000
VERSION     DAORLS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9613_length_4833_cov_1.836961"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..708
                     /locus_tag="PK977_15010"
     CDS             70..708
                     /locus_tag="PK977_15010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HRF19483.1"
                     /translation="MERRELLKMIALVTGSTVIGGELFLTGCKIADKPLVNFTEDIIA
                     LLNEVGETIIPATATPGAKAAEVGKFMKVMVTDCYTEKQQAVFMQGIKDLDDACKKLN
                     GKPFMECSSEQRHEFLVSLEKEAKEFNAKRDKTDEVRKKEHEKKNERLAWKDQKEFEG
                     TPSHYYTMMKQLTLFGFFSSKTGMTETLRHVPSPGRYDGSAPYTKGEKAWAE"
     gene            732..>1417
                     /locus_tag="PK977_15015"
     CDS             732..>1417
                     /locus_tag="PK977_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRF19484.1"
                     /translation="MKYNRRDFIRLTGGLSTGYAFSTSAALSLFKSCDAGSDKLNEFG
                     IQLYTLRADMPKDAAGILKQLAAFGYKEIESYEGPQGMFWGMGHAGFKKHMDELGMRL
                     IASHCDMNKNFEKKVEEAAAIGMKYLICPWLGAQKSLEDYKRHAETFNKKGKKGGLRF
                     AYHNHDYTFKLQEGKLPQDVLMKETDPSLVDFELDIYWLVTAGEDPEVWFKKYSNRFR
                     LCHVKDRSKNP"
     assembly_gap    1418..1499
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1612..>2068)
                     /locus_tag="PK977_15020"
     CDS             complement(1612..>2068)
                     /locus_tag="PK977_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRF19485.1"
                     /translation="VIGLLNMDGSIDLARTAKLVEAAYPLGVTFHRAFDRCKDPFEAI
                     EQLIEIGCERILTSGQQPSVNEGEELIADLNRVADERIIIMPGSGVRVDNIKMLAEKT
                     GCTEFHSSLRGKESSKMSFKHPAFKDSAESYVNNSIDPEQVRALRNSLH"
     assembly_gap    2069..2133
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2134..2346)
                     /locus_tag="PK977_15025"
     CDS             complement(<2134..2346)
                     /locus_tag="PK977_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRF19486.1"
                     /translation="MIYKIEIATSDFSTTQSAVEGGADRIELCANLAEGGTTASFATI
                     KKCREAFSVQLYPIIRPRGGDFLYSKD"
     gene            complement(2380..3417)
                     /locus_tag="PK977_15030"
     CDS             complement(2380..3417)
                     /locus_tag="PK977_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HRF19487.1"
                     /translation="MINRRKFIETSAFGTAGLITAQLGAELSERAGIKGKPIVISTWA
                     PNVKANYAAWEILGMGGRSLDAVVRGVQIPEADPNDNSVGYGGLPDRDGKVTLDSCVM
                     DEKGNCGSVMALEHIMHPVLVARLVMEKTPHVQLVGEGALQFALEQGFKKDNLLTEQS
                     EKAWKEWLKKSNYDPMRSVKEIEEKMEKKKKDNYPWPVALLNHDTIGMVAMDTNGNLG
                     GACTTSGMAFKMHGRVGDSPIIGAGLFVDNEVGAASATGVGEEVVKICGAHTVVEAMR
                     RGLSPELACKEAIRRIVKNKGSQAKDVQVGFLAINNKGQHGAFAVQKGFNYCVTTKDG
                     TKVIDAKCWYP"
     gene            3553..4110
                     /locus_tag="PK977_15035"
     CDS             3553..4110
                     /locus_tag="PK977_15035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRF19488.1"
                     /translation="MKIEIISGSPRAVSVTKRVALHLKNWLRQNTIHEVEIIDMKDWN
                     IPPVQSVWVAPDKAPLEFKPLAERIFSSDAYILVTPEYNGSYSPALKNMLDHFPKRLH
                     KPFGIVTASPGAMGGMRASQQLLQLIPALFGIASPHMLITPAVDKKFSPDGDLLDESF
                     QQAVHTFITEFLWLAEKLVTEEVTA"
     gene            4123..>4833
                     /locus_tag="PK977_15040"
     CDS             4123..>4833
                     /locus_tag="PK977_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRF19489.1"
                     /translation="MKAILHKADTRGHANHGWLDSYHTFSFAGYYNPERVHFGALRVL
                     NDDTVAGGMGFGAHPHDNMEIISIPTFGDLEHQDSMGNKQVIRQGDVQVMSAGTGIQH
                     SEKNRNHDKEVKFFQIWVFPNKRNVEPRYDQQSFTEGDKHNKLLTVVSPIGTNDGGVQ
                     IHQEAWFSLGKLDKDFLTTYTLRSKNNGVYAFVIEGDITVNRHSLTRRDGLAISEAEQ
                     LNIKADSNAEVLLMDVPLI"
BASE COUNT         1408 a          958 c          990 g         1330 t
ORIGIN      
        1 gacatatatg gctataacgg caagggcagc tgatattgct gtaaatgaac taaaaaaagg
       61 aaaattataa tggagcgcag agaattactt aaaatgattg cgttggtaac cgggagtact
      121 gtgataggcg gagagctatt ccttacggga tgtaaaatag ctgataaacc tctggttaat
      181 tttacagaag acattattgc cctattaaat gaagtaggtg aaacgataat tcccgccact
      241 gcaactcccg gggcaaaagc agcagaagtt gggaaattca tgaaagtgat ggttactgac
      301 tgttacacag aaaaacaaca agccgttttc atgcagggaa taaaagacct ggatgatgct
      361 tgtaaaaaat taaatgggaa accatttatg gaatgtagtt ctgaacaacg gcatgagttc
      421 ctggtaagcc ttgaaaagga agcaaaggaa ttcaatgcaa aaagagataa aacagacgaa
      481 gtacgaaaaa aagaacatga gaaaaagaat gaaaggctag catggaaaga ccagaaagaa
      541 tttgagggta ctccgtccca ttattacaca atgatgaaac aacttaccct atttggcttc
      601 ttttcatcta aaacaggtat gacggagaca ctaaggcatg tcccttctcc cggcaggtat
      661 gatggatcag caccctatac taaaggagaa aaggcatggg ctgagtaatt attatttaca
      721 aaaatcagaa aatgaaatac aacagaaggg attttataag gcttacaggc ggtttaagta
      781 caggctacgc attcagtaca tcagctgcac tttcactttt caagagttgt gatgccggtt
      841 ctgataaatt gaatgaattt ggtattcagt tatacacttt gcgtgcagat atgccaaaag
      901 acgctgccgg tatcttaaaa cagttggctg catttgggta taaagaaatt gaaagctatg
      961 aaggcccgca gggaatgttt tggggaatgg gccatgccgg gtttaaaaaa cacatggatg
     1021 agcttgggat gagacttatt gcctctcatt gcgacatgaa taaaaatttt gaaaagaaag
     1081 tagaagaagc tgctgccatt ggcatgaagt accttatttg tccctggctg ggggcacaga
     1141 agtcattaga ggattataag cggcatgcag aaactttcaa taaaaagggt aagaaaggtg
     1201 gattacggtt tgcttatcat aaccatgatt atacctttaa attacaggaa ggaaagctgc
     1261 cgcaggatgt gctgatgaaa gaaaccgacc catctttggt tgattttgaa ctggatattt
     1321 actggctggt gacagcaggt gaagatccgg aagtatggtt taaaaaatat tctaatcgtt
     1381 tcaggctttg ccatgtaaaa gaccgtagca aaaacccnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1501 aagaaaacgg gatgcagcac tttatcgtag aacaggaagc ttatcctaac ggcacaccgc
     1561 tggaagcagc taaagttaat gcggagtata tgaaaaaatt aagagtgtaa attaatgtaa
     1621 cgagtttctc agtgctctta cttgctccgg atcaatcgaa ttgttaacat agctctctgc
     1681 agaatcttta aatgccggat gtttgaaact cattttgctt gactcctttc cccggaggga
     1741 agaatgaaac tcagtacaac cggttttctc tgccagcatt ttaatattat caacccttac
     1801 tccgctaccc ggcattataa taattctttc atctgctact ctgtttaaat cagcaattaa
     1861 ttcctctcct tcattaactg atggttgctg accggaagtg agaattcttt cgcatcctat
     1921 ttcaattaat tgttcaatcg cctcaaaagg atctttgcac ctgtcaaatg cccggtgaaa
     1981 agttactccc aaaggataag ccgcttccac cagttttgct gttctggcta aatcaatact
     2041 cccatccata ttcagaagcc ctatcaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatctttt gaataaagaa aatcaccgcc
     2161 tcttggccgt atgatcgggt ataattgaac agaaaatgcc tcacggcatt ttttaatagt
     2221 tgcaaaagaa gccgtagtgc ctccctcggc cagatttgca caaagctcta tcctgtcagc
     2281 accaccttca actgctgatt gtgtagtaga aaaatctgat gttgcaattt caattttgta
     2341 aatcataggc tgtttggggt taaaagcccc ttatagggat caggggtacc agcattttgc
     2401 atcaattact tttgttccgt ctttggtagt tacacagtaa ttgaatcctt tttgtacagc
     2461 aaaagcccca tgctggcctt tgttattaat tgccaggaat ccaacctgta catcttttgc
     2521 ctggcttcct ttgtttttga caattcttct gatggcttct ttgcaggcta gttcaggtga
     2581 taagccacgg cgcatcgcct cgactacagt atgggcaccg cagatcttta caacttcttc
     2641 acccactcct gtggctgaag cagcaccgac ttcattatcc acaaaaagtc ctgcaccaat
     2701 aataggagaa tctcctaccc ttccgtgcat tttaaatgcc ataccgctcg ttgtgcaggc
     2761 tcctccgagg ttaccgttgg tatccatggc taccatccct atcgtatcat gatttaataa
     2821 ggcaaccggc catggataat tatctttttt cttcttttcc attttttcct caatctcctt
     2881 caccgatctc atgggatcat aatttgattt ttttaaccac tctttccatg ctttttcact
     2941 ttgctcagta aggaggttat cttttttgaa accttgttca agagcaaact gtagcgcccc
     3001 ttcaccaacc agctgtacat ggggtgtttt ttccataacc agcctggcca ctaaaacggg
     3061 atgcatgata tgctcaaggg ccatcacact gccgcagttt cccttttcat ccattacaca
     3121 actgtcgagt gtcacttttc cgtccctgtc aggtaaacct ccgtagccca cactgttgtc
     3181 gttgggatca gcttctggta tttgtacgcc tcttactact gcatccaggg atcttcctcc
     3241 cataccaaga atttcccagg cagcataatt ggccttcaca ttgggcgccc aggtagaaat
     3301 gacaattggt tttcctttta taccggctcg ttcagacagt tcggctccaa gttgcgctgt
     3361 aattaagccg gcggttccga aagccgatgt ttctataaac tttctcctgt taatcattgt
     3421 tttagtttat gcctgccgaa attaacggat tcggtatcaa cgtacgcaaa aacaattaac
     3481 aacctgtgaa gaaataatat ttgatgttta aacattgata tattaatttt acatcctaaa
     3541 cacacaccaa gtatgaaaat tgagatcatt tcaggaagcc cgagagcagt aagtgttacc
     3601 aaaagagtgg cgctccattt aaaaaattgg ttgcgccaaa atacaataca cgaagtggag
     3661 attattgata tgaaagactg gaatattcca ccggttcaat cagtatgggt agcacctgat
     3721 aaagctcccc tggagtttaa acctttagct gaaaggattt ttagttctga tgcttacata
     3781 ttggtaacac ctgaatataa cgggagctac tctcctgcct taaaaaatat gctcgatcat
     3841 tttccaaagc ggctgcataa gccttttggc attgtaacag catctccggg tgccatgggt
     3901 ggtatgaggg cttcacagca attgctgcaa ctgatcccgg ctttattcgg tattgcttcc
     3961 ccgcacatgc tgattacacc tgcagtggat aagaaattca gtccagacgg cgatctgtta
     4021 gatgaaagct ttcagcaggc agtgcatact tttataacgg agtttctctg gctcgctgaa
     4081 aaattagtta cggaagaggt aacagcttaa aaaattagat atatgaaagc aattttacat
     4141 aaagcagata cacggggcca tgccaatcat ggttggttgg attcgtatca tactttcagt
     4201 tttgcaggat attacaatcc tgagagagtt catttcggtg ctttgagggt attgaatgat
     4261 gatacagtag ccggtggtat gggttttggc gcccatccgc atgacaatat ggaaataatt
     4321 tctatcccca cttttggcga tctggaacac caggacagta tgggtaacaa gcaggtgata
     4381 cgccagggtg atgttcaggt aatgagtgcc ggaacaggaa ttcagcattc tgaaaagaac
     4441 aggaaccatg acaaggaagt aaaattcttc cagatatggg tttttcccaa taaaagaaat
     4501 gtggagcccc gctacgacca gcagagcttt actgaaggag ataagcacaa taagctattg
     4561 acagttgtat ctccaattgg aaccaatgat ggtggcgttc aaattcacca ggaagcctgg
     4621 ttcagcctgg ggaaactgga taaagacttt ttgaccactt atactttgag aagtaaaaat
     4681 aatggtgttt atgcttttgt aattgaagga gatattacag ttaacagaca ttcactcacc
     4741 agaagagatg gcctggccat ttctgaagca gagcaactaa acataaaagc tgacagcaat
     4801 gctgaagttt tattaatgga tgttccgctg ata
//
LOCUS       DAORLS010000234         4763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9811_length_4763_cov_2.252761, whole genome shotgun sequence.
ACCESSION   DAORLS010000234 DAORLS010000000
VERSION     DAORLS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9811_length_4763_cov_2.252761"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /gene="surE"
                     /locus_tag="PK977_15045"
     CDS             complement(<1..519)
                     /gene="surE"
                     /locus_tag="PK977_15045"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRF19490.1"
                     /translation="MAKKSNPTPKKKAPEKAKTRKASVKKEKPVILITNDDGVTAPGI
                     MNLVEAVKDLGKIIVVAPDKPQSGMGHAITIGQPLRLHKIHSMENVETWSCTGTPVDC
                     VKLAVDKVLQHKPDLCLSGINHGANHSINVIYSGTMSAAVEAAIENIPSAGFSLLDYR
                     IDADFTGAKKYVK"
     gene            625..1374
                     /locus_tag="PK977_15050"
     CDS             625..1374
                     /locus_tag="PK977_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRF19491.1"
                     /translation="MAISNQNLKYLRKLRGWTQEEFAQKLRIKRSLLGAYEEERAEPR
                     IDVLEVVCDMFKLTLDDMLRKDLSDNKSNYLARRRAMKLVGGRADIPFVPVKAAAGYL
                     AGYADPEFIDELNTFTLPMLTGGNYRAFEIIGDSMLPTPSGSVIVGEKVDSLEEVKNN
                     TPCIVVSRNEGIVYKRVQKNTKQKNKLTLISDNPTYHPYTVNADEVLEMWQAQVVISR
                     TSSQNRWDMAQLATVVSDLQQQVSTLKKKMN"
     assembly_gap    1700..1813
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1814..3935
                     /locus_tag="PK977_15055"
     CDS             <1814..3935
                     /locus_tag="PK977_15055"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF19492.1"
                     /translation="SVFFDTYKDLQNGFQFTVTTANVQSDARLTPNQETDFDEYGDKT
                     WEAVWESKVSIKEDGWVVEMRIPYISLRFAKKDLQDWGLQFLRFTRRTNERDFWNPIN
                     PQINGFVNQFGIYAGLKDIKPPLRLSFSPYISGGYRRSPEAGEFTDTWLRNGGMDVKY
                     GLNESFTLDATLIPDFGQVISDNVINNLSPFEVRFQENRPFFTEGTELFNKAGLFYSR
                     RVGKVPTGYNSVRNFVHNNSSDWEIIKNPSVTQLYNGTKFSGRNKQKIGIGIFNALTA
                     PMDAKIRNKITGKDSVIRTEPLANYNIIVIDQALKGRSFVSLTNTNVLRSGGDRDANV
                     TAVDFALYDRKNIYALNGTARYSKIWGTNPYDGYNTTLRFAKVSGNWQYNVTGNIESA
                     NYDPRDLGFLAAPNEITYRGGLSYRMFTPTKNFLTYSYSLSSRLTYLYKPYAYSYFDV
                     AATGFWVFKNFWDVTLKAVWNPMPVDDYFVLQTPWKFIHYPENYIFSLDGSSDSRRKL
                     FFRYGGVYAKSPEFDNTYTSLSIGFRYRFNDRFTLDLQTVSTYEENQLGYAFLRESNG
                     DPIAGFRDNRDFTSVLSGIYNFTPRINLTLRTRHYWNKVNYKSFHNVDDDGLLSSRPF
                     INGNDDNVNFFNLDAFLTWDFRLGSRLILGYKNWLGEDEYVTPTGKNTYLQNLGDVFD
                     LRHGNEFTLRFIYFLDYNQLRRKR"
     gene            complement(3932..4639)
                     /gene="pdeM"
                     /locus_tag="PK977_15060"
     CDS             complement(3932..4639)
                     /gene="pdeM"
                     /locus_tag="PK977_15060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HRF19493.1"
                     /translation="MIKPIHLALYLPASMQNPVPHIIHDQIFWITPERCIFWEEENTL
                     IVADLHLGKTGHFRKAGIAVPQSIYKADLQRLMAMLYLFKAERLIVVGDFTHSTANKE
                     LELFLKWRKDFSALQIDLVKGNHDILEDAWYKEANVILHEWELLINGYLFKHEDKRYK
                     RPEVENAKYTFSGHLHPGFKLKGQGKQSLKFPCYFFGKYHCILPAFSRFTGTFLVEPK
                     KGDVVYAITGAELLKIG"
BASE COUNT         1323 a          898 c         1011 g         1417 t
ORIGIN      
        1 ctttacatat tttttggcgc cggtaaaatc agcatcaatt cgataatcca gcagggaaaa
       61 acctgccgag ggtatatttt caatggcagc ttctacagcc gcagacatgg tgcctgaata
      121 aatcacattg atactatgat tggcaccatg gttgatgcca ctcaggcaaa gatcaggttt
      181 gtgttggagt actttgtcca ctgccaattt tacacaatca acaggagtac cggtacatga
      241 ccatgtttca acattttcca tggagtggat cttgtgcagc cgcagcggct gaccaatggt
      301 gatagcatgt cccattccgg actgaggctt atcgggggct actactataa tttttcccag
      361 gtcttttaca gcttctacca ggttcatgat accgggagca gttacaccgt catcgttggt
      421 gatcagaata accggttttt cttttttcac tgaggctttc cgggttttgg ccttctctgg
      481 ggcctttttc ttaggagttg ggtttgattt ttttgccata cgatacaaaa ctcccggttt
      541 tttccgggtt atccaaaata gccatattat ctgttttgaa attctaaata aattagtatt
      601 ttgcggctaa ttaaattagt gattatggca atttccaatc aaaacctgaa atacctgcgt
      661 aaacttcgtg gctggacgca ggaagaattt gcacaaaaac taagaataaa aagatcactg
      721 ctcggcgcct atgaagagga aagggcggaa ccacggattg atgtgctgga agtggtttgt
      781 gatatgttca agctgacatt ggatgatatg ttgcgaaaag atctcagtga caataaaagt
      841 aattatctcg ccaggcgcag ggctatgaaa ctggtgggtg gcagggcaga tatacctttt
      901 gtacctgtaa aagctgctgc tggttatctc gccgggtatg ctgatcccga gttcattgat
      961 gagttaaata ctttcaccct tcccatgctt actggcggaa attacagggc gtttgagatc
     1021 atcggagatt ctatgctgcc aacgcccagt ggttctgtaa tagtgggaga aaaagtggat
     1081 agtttggaag aagttaaaaa taatactccc tgcatagtcg tatctcgtaa tgagggtatc
     1141 gtatataaga gagtacaaaa gaacaccaag cagaaaaata aactgacttt gatcagtgat
     1201 aatcctactt accatcctta cacggtgaac gccgatgagg tattggagat gtggcaggcc
     1261 caggtagtaa tttcacgtac cagcagccaa aacagatggg atatggctca attggccacc
     1321 gtagtttctg atctgcagca gcaggtttct acgctcaaaa agaaaatgaa ctaggcttcc
     1381 agggtattta ttttattaat tatgcgtttt tgtgcggctt gtttctttcc tttcttccgg
     1441 taagctatat ttgtaagctg taaactgctg catgaggctt aaagctatta tctgtttacc
     1501 ctttttactg gtttgtgtaa acctttattc ccaggttaaa acattgcaag ccataaaaat
     1561 aagtcaggca ccaaaaattg atggtaacct ggacgacgcc gcatggataa acgtaactcc
     1621 tgcttcaaac tttatccaaa actttccgac ctatggtaag gaagcttcta ctaaaactga
     1681 agtgaaactg gtttatgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnttctgtt ttttttgata cctacaaaga tttacagaat ggctttcagt
     1861 ttacggtgac gactgccaat gtacaatctg atgcccgcct tacacccaac caggaaaccg
     1921 atttcgatga atacggggat aaaacctggg aagcggtttg ggaaagcaag gtaagtatca
     1981 aagaggatgg ctgggttgtg gagatgagga taccttatat ctcacttcgt tttgccaaga
     2041 aagatttgca ggactggggc ctgcagtttc ttcggtttac caggagaacg aacgaaaggg
     2101 atttctggaa tcctataaat ccacaaatca atggatttgt taaccagttt ggtatttatg
     2161 ccggtttaaa agacattaaa ccaccgcttc gccttagttt ctctccctat atatcaggcg
     2221 gctaccgccg ctctcctgaa gccggtgagt tcactgatac ctggctacga aacggaggaa
     2281 tggatgtgaa gtatggcctg aatgaaagct ttacattgga cgctaccttg attcctgatt
     2341 ttggccaggt aatttctgat aatgtcatca acaacctgtc tccatttgaa gtaagattcc
     2401 aggaaaacag gccatttttt acggagggaa cagagctatt caataaagct ggtctttttt
     2461 attcaagacg ggttgggaaa gtgcctaccg gttataactc cgtaagaaat tttgtacata
     2521 acaacagtag tgattgggag ataattaaaa acccttctgt tacgcagtta tataacggta
     2581 caaaattctc tggcagaaat aagcaaaaga ttggtatcgg gatctttaat gctttgacgg
     2641 caccaatgga tgcaaagatc aggaataaga ttaccggtaa agattcagta atccgcacgg
     2701 agccgttggc taactataat attatcgtta ttgaccaagc attgaaaggc cggtcattcg
     2761 tttccctgac caacacaaat gttctaagaa gcggtggcga cagggatgcc aatgtaactg
     2821 cagttgattt tgctttatat gataggaaaa atatttatgc tttgaacgga acagcccgtt
     2881 atagtaaaat atggggaaca aatccttatg atgggtataa tacaaccttg cgttttgcca
     2941 aggttagtgg taattggcaa tacaatgtaa ctgggaatat agaatctgcg aattatgatc
     3001 caagggacct cggtttcctg gcagcaccta atgaaataac ctacagggga ggactttctt
     3061 atcgcatgtt tactcctaca aagaattttt tgacttacag ttatagcctg agttcaaggt
     3121 tgacttactt atacaagccc tatgcttact cctattttga tgtggctgca acgggttttt
     3181 gggtgtttaa aaatttctgg gatgttacac ttaaggccgt gtggaatccc atgccggttg
     3241 atgattattt tgtattgcaa actccctgga aatttattca ttaccccgag aattatattt
     3301 tcagcctgga tggaagttcg gacagcagaa gaaaactatt tttccggtat ggtggtgtat
     3361 atgccaagag cccggaattt gataatacct atacatcatt aagtattggt ttccggtacc
     3421 gttttaatga caggtttact ctcgatttgc agactgtatc aacttatgaa gagaaccaat
     3481 taggttatgc ctttctgaga gaaagtaatg gtgatcctat tgctggtttc agagataaca
     3541 gggattttac ttctgtactt tccggcatat ataattttac tccacggatc aatcttacat
     3601 tgagaacaag gcattattgg aacaaggtga attataaaag ctttcacaat gtagatgatg
     3661 atgggttgct gagcagccgt ccatttatta atggaaatga tgataatgtg aacttcttca
     3721 atttagatgc attccttacc tgggatttca gattaggcag ccgtttgata cttggctata
     3781 aaaactggct gggtgaggat gaatatgtga cgcctacagg taaaaacact tatttacaaa
     3841 atcttggaga tgtttttgat cttaggcatg gaaacgaatt cacactccgc tttatttatt
     3901 tcctggatta caatcaactt cgcaggaaac gttaaccaat cttcagtagc tctgccccgg
     3961 taattgcata taccacatcg ccctttttcg gctcaactaa aaaagtgcct gtaaagcgac
     4021 taaaagcagg cagtatacag tgatattttc caaaaaaata gcaaggaaat ttcaggcttt
     4081 gttttccttg tcccttgagt ttaaaaccgg gatgcagatg gcctgagaaa gtatatttcg
     4141 cattctcaac ttccggtctc ttgtaacgtt tatcctcatg tttgaaaagg tatccgttga
     4201 tcaataattc ccattcatgc aaaattacat ttgcctcttt gtaccaggca tcttccaaaa
     4261 tgtcatgatt gcctttgaca agatcaatct gtagcgcaga gaaatctttt ctccatttca
     4321 ggaaaagttc cagttcttta ttggcagtac tgtgtgtgaa atcgcctact acaatcagtc
     4381 tttctgcctt aaaaaggtac agcatggcca ttaatcgttg caggtctgct ttgtaaatgg
     4441 attgaggaac ggcaatcccg gctttacgaa agtggcctgt ttttcccaaa tgaaggtctg
     4501 ctacaatcag tgtgttttct tcttcccaga aaatacatct ttccggggtt atccaaaata
     4561 tctggtcgtg gatgatatgg ggaacagggt tttgcatgga tgccggcaaa tataaagcaa
     4621 gatgaattgg tttaatcatc aggctgttta ctaactactt tccagttgct gttgcattct
     4681 ttttacacgg tcctccagtt tttctgagct gagatcttcc cgcatactgt caacttttat
     4741 cgggaaacaa aacggtgtta gtt
//
LOCUS       DAORLS010000235         4761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9815_length_4761_cov_2.436464, whole genome shotgun sequence.
ACCESSION   DAORLS010000235 DAORLS010000000
VERSION     DAORLS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9815_length_4761_cov_2.436464"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>254)
                     /locus_tag="PK977_15065"
     CDS             complement(<1..>254)
                     /locus_tag="PK977_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19494.1"
                     /translation="GNWGHNNKFFTGKNGPLKLYVKDFDGNGSVEQIMCYTIDGKEYT
                     FLAKDELERPLPVLKKSYLNYSDVAGKTVQYMFYNLFKDYL"
     assembly_gap    255..265
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<266..2836)
                     /locus_tag="PK977_15070"
     CDS             complement(<266..2836)
                     /locus_tag="PK977_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF19495.1"
                     /translation="MFESLPASKTNIDFTNKLKEHQLFSILYYLYYYNGGGVATGDVN
                     NDGLPDIYFTANNTSGNKLYLNKGNFQFEDVTAKAGVAGKSDWCTGVTMADVNGDGWL
                     DIYTSAISSKYNLKGHNELFINNGNGTFTESAAEYGLAFSGLTTQSAFFDFDRDGDLD
                     CYILNQSHHPHANIVDTSNRRKYDPMSGDILLRNDVAATGKFTDISKAAGIYQSNLGY
                     GLGLAIADFNNDGWDDIYIGNDFHENDYYYINNGNGTFSESGAQHFKHYSRFSMGNDV
                     ADYNNDGQLDIITLDMLPPDEKILKTYGSDENPDIYKVKLEMHGYQKQYSKNCLQRNN
                     GNGSSFSETALISGVSATDWSWAPLFADFDNDGNKDLFISSGIVKRPVDLDYIRFVSD
                     LDMRKGRQQTDKYDDMTIENMPDGSSHPFFFKGDGKLKFEDVSKAWGTAKMKGYFNGA
                     AYADLDNDGNLDVVINCIDAPAVVLKNNAPAKTMVNLSLKGEGMNKFGVGAKVWMFTG
                     KTMQYQQMMPTRGFQSSVDMRLHFGLDSVKYIDSILVVWPNQKYQLLKNVPAKGDMQL
                     KEMESGGVFNYNEWFPPAKQSFENITGQIALNWAHRENEFLDYNKQYLIPHQQSTRGP
                     KIAVADVNKDGLDDFYVCGAAGQPGQLMIQSAGGQFASVDTAIFGKAKDSEEVDAVFF
                     DANNDGNPDLYVVSGGNEYYDGTPVLADNLYMNDGKGHFKRTENQIPSLLKNKSTVSV
                     SDIDHDGDTDLFVGCLADARKYGIPQTSWLLLNDGKGTFTQAASSVIKLDSIGMITTS
                     TFTDLNKDGWDDLVVTGEWMPLKIFFNNKGVFTENNISQSTGLWQTLLPADVNGDGNT
                     D"
     gene            complement(3049..3894)
                     /locus_tag="PK977_15075"
     CDS             complement(3049..3894)
                     /locus_tag="PK977_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRF19496.1"
                     /translation="MKNNLKTILSVALVAFVAISCQKMDKPELGNYPVDANPPNGPLK
                     FYVAFDGTTTNPLMNAVDSIRANFPSENPFTTVDGIKGKGVTGVNQKFIKYPSFNSWA
                     SSSSFTISIWYKKDGQTKNNNGTNGPEYLLSMRAVNNFHWSNAVGFVFLEGNNTACAV
                     KTMFVSNTGDAWFTWEGGQSIAGLCDNNWHHLVLTYDETTSQVKLYIDGVQNPNVKTW
                     GGHGALNLATSKISEFRIGAGPSTSYASDDWLACTFKGSLDQLRMYSTVLTQAEIQAL
                     FTAKQ"
     gene            complement(3926..>4761)
                     /locus_tag="PK977_15080"
     CDS             complement(3926..>4761)
                     /locus_tag="PK977_15080"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF19497.1"
                     /translation="QQWQPALDLCKEVMAARQGGASAPLYTPYWKIFKKEGELSEESI
                     FEIQAHKTAGDGNTYWSRLGQCQGVRGGSGAWDLGWGWNTPTSNLVGSYEAGDIRKDA
                     TILFSNQSDGGLNTGGYGQTLPNLSNALYWNKKVYNLYSDYLAAGLGTPNNEAQNTWV
                     NHRVFRMADVYLMAAEAANELGGAQNQADARTWVNMVRTRAGLPNIAFVSQAQFRDAV
                     KQERRAEFAMEFERFFDLVRWGDALSVLGPLGYQNKHRFYPIPTSALNSNPNLVQNPE
                     W"
BASE COUNT         1344 a         1131 c          871 g         1404 t
ORIGIN      
        1 aggtagtctt tgaaaagatt gtaaaacatg tactgaaccg ttttacccgc cacgtcgctg
       61 taattcaaat atgatttttt taaaacaggc agcggtcttt ctaactcatc ctttgcaagg
      121 aatgtatatt cctttccgtc tatcgtatag cacataattt gttctaccga accgttacca
      181 tcaaaatcct ttacatacaa ttttaatggt ccgtttttac cggtaaagaa tttattatta
      241 tgcccccagt taccnnnnnn nnnnnatctg tattgccatc accattcaca tcagcaggca
      301 atagtgtttg ccataaaccg gtagactggc tgatattatt ttcagtaaat actcctttgt
      361 tattaaaaaa tatcttcagc ggcatccatt ccccagttac caccaggtca tcccagccgt
      421 ctttattcag atcagtaaaa gtggaagtag ttatcattcc gatactatcc aacttaatca
      481 cagaagaagc agcctgtgtg aaagtgccct tcccatcatt cagtaatagc cagctcgttt
      541 gagggatacc atatttcctg gcatcggcca ggcatcccac aaaaagatca gtatctccgt
      601 catgatctat atcagaaaca ctgactgttg acttattttt caacagtgag ggaatttgat
      661 tttctgtacg cttaaaatga cctttcccat cattcatgta taagttatct gccagtacag
      721 gtgtgccgtc ataatattca tttccgccac tgacgacata caaatcaggg ttaccatcat
      781 tgtttgcatc aaagaaaaca gcatccacct cttcgctgtc ctttgccttg ccaaaaatgg
      841 cagtatcaac agaggcaaat tgtccgccag ctgattggat cattagctga cccggttgtc
      901 ctgcagcgcc acatacataa aaatcatcca gcccatcctt gtttacatca gccactgcta
      961 ttttcggccc tcttgttgat tgctggtgag gaataaggta ttgtttatta taatcaagaa
     1021 attcattttc ccgatgcgcc cagttcaatg caatttgccc ggtaatattc tcaaatgatt
     1081 gctttgccgg ggggaaccac tcgttataat taaatacacc tcctgattcc atctctttca
     1141 gctgcatatc tcccttggcc ggaacatttt taagtagctg atacttttga ttgggccaca
     1201 caaccaggat gctgtcaata tatttaacac tgtccagccc aaaatgcaac cgcatatcaa
     1261 ccgaagactg aaagccccgg gttggcatca tttgctggta ctgcatggtt ttgcctgtaa
     1321 acatccacac tttagcgccg acgccaaact tgttcatccc ctctcctttc agagaaagat
     1381 tgaccatcgt tttagccggg gcattatttt ttaagactac agccggtgca tcaatacaat
     1441 taataacaac atccaggttt ccgtcattat caagatcagc ataggccgcc ccgttaaaat
     1501 aacctttcat ttttgcagtg ccccatgctt tgctcacatc ttcaaacttt aattttccat
     1561 cccctttgaa aaagaaagga tgcgaactgc catccggcat attctcaatg gtcatatcat
     1621 catacttatc tgtttgctgc cggcctttac gcatatccag atcagaaaca aatcggatat
     1681 aatcgagatc aacaggtctt tttacaattc cacttgaaat aaacagatcc ttatttccat
     1741 cattatcaaa atcagcaaac aaaggagccc agctccagtc ggttgcagat acaccgctga
     1801 taagagctgt ttcactaaag ctgctgccat taccgttgtt tcgctgaaga cagtttttgg
     1861 aatattgctt ctggtagcca tgcatctcca gctttacttt gtagatatca ggattttcat
     1921 cgcttccgta agttttcaaa attttttcat caggaggaag catatccagc gtaataatat
     1981 ccagttgccc gtcattatta taatcagcca catcgttacc catactgaag cggctatagt
     2041 gtttaaaatg ttgtgctccg ctttctgaga aagtaccgtt gccgttattg atgtagtaat
     2101 aatcattttc atgaaaatca ttgccaatgt atatgtcatc ccacccgtca ttattaaaat
     2161 cagcaatggc cagtcccagc ccatacccaa ggttactttg atatattccg gcagcttttg
     2221 aaatatcggt aaattttccg gtggcagcga catcattacg gagtaaaata tctcccgaca
     2281 tggggtcata ttttctcctg ttagaagtat ctacaatatt ggcatgggga tgatgggact
     2341 ggtttagtat ataacaatcc aggtctccat ctctgtcaaa atcaaaaaaa gctgattgag
     2401 tagttagtcc ggaaaaagcc agtccatact cggctgcact ttcagtaaaa gttccgttac
     2461 cattattaat gaataattca ttatggcctt tcaggttata ttttgagctg atggctgagg
     2521 tgtaaatatc caaccagcca tcgccattta catcagccat cgttacaccg gtacaccagt
     2581 ctgattttcc agccacacct gcttttgccg taacatcttc aaattgaaaa ttgcctttgt
     2641 tcaggtacag cttattacca ctggtattat tggccgtaaa atatatatcg ggcaacccat
     2701 cattgtttac atcaccggta gccacaccac cgccattata ataatagaga taatagagaa
     2761 tactgaagag ttgatgctcc ttcagtttgt tggtaaaatc aatatttgtt ttggaagcag
     2821 gcagcgattc aaacatggaa ggttttttac tgctgcaagc agataacaat agaacaaaaa
     2881 aagaaagcaa ttgcgaaaca agatatagag gcaccctttt atctggcata aaaaactaat
     2941 attaggccct gttgaaagaa taaaagggcc aaaacaagat gttcagccct tttgtgttgc
     3001 ttatgtctgc ttaaaacaaa acgagaaatg agaaaaacct ttcgactatt attgcttggc
     3061 agtgaacaat gcctgaattt cagcctgtgt taacacggtg ctgtacatcc gcaactgatc
     3121 aaggcttccc ttaaacgtac atgccagcca gtcatcggaa gcgtaacttg tagatggtcc
     3181 tgcaccgatt ctgaattcac tgatcttgct ggtagcaagg ttcagagcgc catgaccacc
     3241 ccatgttttt acatttggat tctgcacacc gtcaatatac aatttcacct ggctggttgt
     3301 ttcatcatat gttagaacaa gatgatgcca gttgttatca cataacccgg caatgctctg
     3361 accaccttcc caggtaaacc aggcatctcc ggtattgctt acaaacattg ttttaacagc
     3421 acaggcagtg ttattaccct ccaggaaaac aaacccaacg gcattgctcc agtgaaaatt
     3481 attcactgcc ctcatagaaa gaaggtactc cggaccgtta gtaccattgt tgttcttagt
     3541 ctgtccatcc tttttatacc agatagaaat agtgaaactg ctgctggatg cccatgaatt
     3601 gaacgaaggg tacttaatga acttctggtt aacacctgta acacctttac cctttattcc
     3661 gtcaaccgta gtaaatggat tttccgaagg gaaattagcc ctgatgctgt ctaccgcatt
     3721 catcaacgga ttagtggtag ttccatcaaa agccacataa aactttaatg gtccgttggg
     3781 agggttagca tctaccgggt aatttcccag ctcaggctta tccattttct gacaactgat
     3841 tgcaacaaag gctaccagcg caacggacag aattgttttt aagttatttt tcatacttca
     3901 agttttttat aaccgttaaa gaattttacc attcagggtt ctgcaccagg ttaggatttg
     3961 aatttaacgc tgatgtcgga ataggataga aacgatgctt attctgatag cccaaaggac
     4021 caagtacact caaagcatca ccccatctta ccaggtcaaa gaaacgctca aactccatcg
     4081 caaactcagc tcttctttcc tgcttaactg catccctgaa ctgggcctgg cttacaaaag
     4141 caatattggg caacccagct cttgtacgaa ccatattgac ccaggttctt gcatccgcct
     4201 ggttttgtgc accacctaac tcattggcag cttctgcagc catcaggtaa acatcagcca
     4261 ttctgaatac cctgtgattt acccaggtat tttgggcttc gttattaggc gttcctaaac
     4321 cagcagccag gtaatcgcta tagagattat aaaccttttt attccagtaa agtgcattgg
     4381 aaagattagg caatgtctgg ccataaccac cggtgttcag gcctccgtcg ctttggttgg
     4441 aaaacaaaat agtggcatct tttctgatgt cgcctgcctc atacgaacca acaaggttag
     4501 aggtaggtgt attccagccc cagcctaaat cccatgcacc tgatccgcct cttacaccct
     4561 ggcactggcc taaacggctc cagtaagtgt taccatcacc cgcagtctta tgcgcctgga
     4621 tttcaaaaat tgattcctca ctcagctcgc cttctttctt gaatattttc cagtaaggag
     4681 tatacaaggg agcagatgca ccaccctggc gggctgccat tacttcttta caaagatcaa
     4741 gggcgggctg ccactgttgc c
//
LOCUS       DAORLS010000236         4735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9881_length_4735_cov_1.956624, whole genome shotgun sequence.
ACCESSION   DAORLS010000236 DAORLS010000000
VERSION     DAORLS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9881_length_4735_cov_1.956624"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK977_15085"
     CDS             <1..507
                     /locus_tag="PK977_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRF19498.1"
                     /translation="AGDISALSTVRTAHPDKNLYFTEQWTGANGSFNGDLAWHIKNVI
                     IGSMRNWSRNALEWNLANDPAFGPHTPGGCSECKGAITISGSAFSRNVSYYIIAHASK
                     FVPAGSVRIASNMATNLPNVAFKTPSGKKVLIVLNESGTGQGFNIRYKGKWVFTSLAA
                     GAVGTYVW"
     assembly_gap    653..667
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <668..2029
                     /locus_tag="PK977_15090"
     CDS             <668..2029
                     /locus_tag="PK977_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HRF19499.1"
                     /translation="GKKVIVYTTADSTDYRLSVTDTVEFKDMGQPFETQVCVFVDPSK
                     TFQTYFGIGAALTDASAETFYKLPKETQDELLKAYFDKEKGIGYTVARTNINSCDFSS
                     DMYTYVQDGDKELKTFNIEHDRKYKIPFIKEAMKTAGGKLNLFASPWSPPAWMKDNND
                     MLQGGKLKPEFYDSWAMYYTKFIKEYEKEGVPIWGISIQNEPMAKQRWESCIYTAEEE
                     RDFLKKHLGPTMEKEGLKDKKIIVWDHNRDLIYQRAQTYFDDAEASKYIWGIGFHWYE
                     DWSGGLPMYDNLRRVYETFPDKHIFFTEGCAESFNASRYNAWVLGEEYGRSMINDFNN
                     GMVGFTDWNILLDETGGPNHVQNFCFAPVHGDTKTGKLIYTNAYYYIGHFSKFIQPGA
                     KRVTAAASRSQLLTTAFKNEDGKTVVVVMNQSNIKTPYYLWINGKAAEITALPHSIAT
                     LVF"
     gene            2041..3732
                     /locus_tag="PK977_15095"
     CDS             2041..3732
                     /locus_tag="PK977_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRF19500.1"
                     /translation="MKKLNRQYLLVFLAPLFLAANCNKKDSSPEPPPPPPSPLVFTGA
                     RLDNTGALTAATNYNVRPGVSIKLSFNNAVDRSTTSTAVSLKENGTTTVAVNYTYENN
                     DSTVVITPTASLKYLTRYVVTAITSLKSSKGGSLLTQATVTIITQIDSTDKFPLISDN
                     ALLDLVQQQTFKYFWDYGHPVSGLARERSNATPETVTTGGSGFGIMGIPVAIQRGFIT
                     RAQGLSRMQTIVSFLKNTAQKFHGAFPHWMNGTTGVVIPFSQKDNGADLVETSFMVAG
                     LLTVRQYFDGADPAETTLRTDINTIWNGVEWNWFRKSNENVLYWHWSPNYNWDMNHKI
                     QGWNECLITYIMAASSTTYGIPQAVYREGFARNGAMINNSTYYGYQLPFGPSNGGPLF
                     WAHYSFLGINPNGLTDIYGNYQTQVTNHTKINYEYCKANPTGYFGYSNQCWGLTASDV
                     PSGYNANEPNNDIGVISPTAALSSFPYTPAESMNALKFFYYKLGDKIWKQYGFVDAFS
                     LKDLWYADSFLAIDQGPIIVMIENYRSGLVWNLLTSCPEIKNGMKNVLGFAAPYL"
     gene            3743..>4363
                     /locus_tag="PK977_15100"
     CDS             3743..>4363
                     /locus_tag="PK977_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HRF19501.1"
                     /translation="MNKIFSIAIVLLLVAGCNSGKKASMSKGEGVLSAEDTVLFRKVQ
                     QQTFQYFWDGAEPVSGLGRERFHVDDKYPDNDKNVVTSGGAGFGVMAILVGIERGFVT
                     REAGRKHLEKIVRFLETADRFHGAWPHWWNGETGKVKPFGKKDDGGDLVETSFMLQGL
                     LCVRQYFQNGNKEEKALAARADKLWKEVDFNWYRNGQNILYWHWSPN"
     assembly_gap    4364..4522
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4523..>4735
                     /locus_tag="PK977_15105"
     CDS             <4523..>4735
                     /locus_tag="PK977_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRF19502.1"
                     /translation="NGIAANYPYKLQMKHQGDAWNGGPLFWAHYSFLGLDPRGLKDRY
                     ADYWEECRNQALINYQWCVDNPKRYKG"
BASE COUNT         1374 a          858 c         1091 g         1238 t
ORIGIN      
        1 gcgggtgata tcagtgcatt gtccactgtg cggacagcac accctgataa aaatttatac
       61 tttactgaac agtggaccgg ggccaacggc agttttaacg gagacctcgc atggcatatc
      121 aagaatgtaa tcattggatc catgcgaaac tggagccgta atgcgttgga atggaatttg
      181 gccaatgacc cggcttttgg tccgcataca cccggaggct gttcagagtg taaaggtgcc
      241 atcactatca gcggttctgc tttcagccgt aacgtcagtt attatattat agcccatgca
      301 tccaagtttg ttccggctgg ttcagtcagg attgccagta acatggcaac caacctgccc
      361 aatgttgctt ttaaaacgcc ttccggtaaa aaagtgctga ttgttttgaa tgaaagcggt
      421 acaggacagg gtttcaatat ccggtataag ggcaagtggg tgttcacttc gctggctgcc
      481 ggtgctgtgg gtacatatgt ttggtgagaa tatttgttca acatgattaa tacacgactt
      541 aatatcagct ttatgaaaaa tttgttaccg gtattggttt tggtcgcagc tatttcctgc
      601 acagacaaaa cagaaaaaca atcagcttct gcaacaacaa ctcctttttc aannnnnnnn
      661 nnnnnnnggg aaaaaagtga ttgtatatac tacggcagat agtacggact acaggctttc
      721 tgtgaccgat acggtggagt ttaaagatat gggacaacct ttcgaaacac aggtatgtgt
      781 ttttgtagat ccctctaaaa catttcagac ttattttggt attggcgctg cattgacaga
      841 tgcatcagct gaaacttttt ataagctgcc aaaggaaaca caggatgaat tgctaaaagc
      901 ttatttcgat aaagaaaaag gaattggcta cacagtggcc cgtacgaata ttaacagctg
      961 tgattttagt agtgatatgt acacttatgt gcaggacgga gataaggaat taaaaacctt
     1021 caacattgag catgacagga agtataaaat ccctttcatc aaagaagcaa tgaaaacagc
     1081 aggtggtaaa ttaaacctgt ttgccagtcc ctggagccca ccggcctgga tgaaggataa
     1141 taatgatatg ctgcagggcg gaaaactgaa gcctgagttt tatgacagct gggccatgta
     1201 ctacactaaa ttcatcaaag agtatgaaaa ggaaggtgtg ccgatctggg gcatcagcat
     1261 tcagaatgag ccgatggcca agcaacgctg ggaaagctgt atttatactg cagaagaaga
     1321 aagagatttt ttaaaaaagc atttaggccc tactatggag aaagaggggc tgaaagataa
     1381 aaaaatcatt gtgtgggacc ataaccgtga cctgatctac cagagagcac aaacttattt
     1441 tgacgatgct gaggccagta agtatatctg gggaatcggt tttcactggt atgaagactg
     1501 gagtggtggt ttgcccatgt acgataatct gagaagggta tatgaaacat ttccagataa
     1561 acacatattc tttaccgaag gttgtgcaga aagtttcaat gccagccgct ataatgcatg
     1621 ggtgctggga gaagaatatg gccgcagtat gatcaatgat ttcaataatg gtatggtcgg
     1681 ttttactgac tggaatattt tattagatga aacaggaggc cctaatcatg tacagaattt
     1741 ttgttttgcg ccggtacatg gtgatacaaa gactggaaag ctgatctata ccaatgccta
     1801 ttattatatt ggtcattttt ccaaattcat tcaacccggg gccaaaaggg taactgctgc
     1861 agccagccgc agccagttat taactactgc tttcaaaaac gaagatggaa aaacggtagt
     1921 agtagtgatg aatcaaagta atataaagac gccttattac ctgtggatta atggtaaggc
     1981 agcggagata acagcattgc ctcattctat tgcaacactg gtgttttaaa aaatcatgta
     2041 atgaaaaagc tgaaccgtca atacctgctg gtgttcctgg cgcctttgtt tttggctgcc
     2101 aactgtaaca agaaagacag cagtccggag ccgccgccgc caccaccttc tccattggta
     2161 tttaccggtg caagactgga taatacagga gcattgacag cagcaaccaa ttataatgta
     2221 agacccggag tatccataaa gctttcattc aataatgcag ttgatcgttc tactaccagt
     2281 actgctgtat cgctgaagga aaacggcacc actacagttg ctgttaatta cacttatgaa
     2341 aacaatgaca gcacagtggt tattacccca acggcatcgc tgaaatacct gacccgttat
     2401 gtagttacag cgattacttc tcttaagtca tcaaaaggcg gcagcctgct tacacaggca
     2461 acggttacca ttattacaca aattgactcc acagataaat ttcccctgat cagtgataat
     2521 gctttgcttg atttggtgca gcagcaaacg ttcaaatatt tctgggatta tggtcatcct
     2581 gtaagcggtc ttgcaagaga aagaagtaat gcaactccgg aaactgttac gacaggtggt
     2641 tcgggatttg gtataatggg tataccagtt gccatccagc ggggatttat cacaagagca
     2701 caaggcttat caagaatgca aactatcgtt tcatttctaa aaaacacagc acaaaaattt
     2761 catggagctt ttccgcattg gatgaatgga actaccggtg ttgtcatacc gtttagtcag
     2821 aaagataatg gcgccgacct ggtggaaact tcctttatgg tagcagggtt attaactgta
     2881 agacaatatt tcgacggtgc tgatccggca gaaacaactt tgcggacaga tatcaatacc
     2941 atctggaacg gagtggaatg gaactggttc agaaaaagca acgaaaatgt gctgtactgg
     3001 cactggagtc ccaactataa ctgggacatg aaccataaga tacagggctg gaatgagtgc
     3061 ctgatcactt atatcatggc agcatcttct acaacttatg gaattcccca ggcagtttac
     3121 agggaaggat ttgcacgcaa cggtgctatg ataaacaaca gcacctatta tggttatcaa
     3181 ttgcctttcg gtccttccaa tggtgggcct ttgttctggg cacattattc tttcctgggt
     3241 ataaatccga atggtctaac agatatttat ggcaactacc agacgcaggt aaccaatcat
     3301 accaagatta attatgaata ctgcaaggct aatcccacgg gatactttgg ttatagcaac
     3361 cagtgctggg ggctgacggc aagcgatgta cccagtggtt ataatgccaa cgagccgaat
     3421 aatgatattg gtgtaatctc gcctacggca gctctttctt catttccata tacacctgca
     3481 gaaagtatga atgccttaaa atttttctat tacaaactgg gtgataagat atggaagcaa
     3541 tatggttttg ttgatgcatt ttctttaaaa gacttgtggt atgctgattc attcctggct
     3601 attgaccagg ggccaattat tgtaatgatt gaaaattatc gttcgggcct ggtttggaac
     3661 ctgcttacca gttgtccgga gattaaaaac ggaatgaaaa atgttttagg ttttgcagcg
     3721 ccatatttgt agctgaatta tcatgaacaa aattttttct atagcgatcg ttttgctgct
     3781 ggtcgccggt tgtaattccg gtaagaaggc tagtatgagc aaaggcgaag gagtattgtc
     3841 tgcagaagat acggttctct tcaggaaggt gcagcagcaa acctttcaat atttctggga
     3901 cggagctgaa ccggtaagtg gccttggccg tgaaagattt catgtagatg ataagtatcc
     3961 tgataatgac aaaaatgttg tgacaagtgg cggcgccggt tttggtgtta tggccatact
     4021 tgttggtata gaaagaggat ttgtaacaag ggaggcggga aggaaacatc tagagaagat
     4081 cgttcgtttc ctggagaccg ctgatcgctt tcatggtgca tggccgcact ggtggaatgg
     4141 ggaaacaggg aaagtgaaac catttggaaa aaaggatgat gggggtgacc tggtggaaac
     4201 atcctttatg ctccaggggc ttttatgtgt aagacaatat tttcaaaatg gtaataaaga
     4261 agagaaagct ttagcagcaa gagcggataa gttatggaag gaggttgatt ttaactggta
     4321 cagaaacggg caaaatattt tatactggca ctggagcccc aacnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnaaatggta ttgctgctaa ttatccttat aaattacaaa
     4561 tgaagcacca gggtgatgca tggaacggag ggccgctttt ttgggcacac tattcatttc
     4621 ttggattgga tccgcgggga cttaaagaca ggtatgccga ttactgggag gaatgcagga
     4681 accaggcttt gatcaattat caatggtgtg ttgataatcc caaaaggtac aaagg
//
LOCUS       DAORLS010000237         4724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9906_length_4724_cov_1.646819, whole genome shotgun sequence.
ACCESSION   DAORLS010000237 DAORLS010000000
VERSION     DAORLS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9906_length_4724_cov_1.646819"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>214)
                     /locus_tag="PK977_15110"
     CDS             complement(<1..>214)
                     /locus_tag="PK977_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19503.1"
                     /translation="KMVKEMVAADKVPPTALWDYLDSDDGFQNSGIAGCEVYKSEDGG
                     ATWKKTNQKPINIFSTYGYYFAKIYVS"
     assembly_gap    215..233
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<234..>599)
                     /locus_tag="PK977_15115"
     CDS             complement(<234..>599)
                     /locus_tag="PK977_15115"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19504.1"
                     /translation="NELYAGMWYRTRRAWKFEESGKTSGIYKSTDGGETWKLISGPGS
                     GFVTGDKIGRIGLAVYPNNPNIVWAVVDNNTPQPDTSKKVDTFYKKADFKNITKEQFA
                     ALKTNLIDTFLRKNGFPRKY"
     assembly_gap    600..617
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<618..1307)
                     /locus_tag="PK977_15120"
     CDS             complement(<618..1307)
                     /locus_tag="PK977_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19505.1"
                     /translation="MVSKLGQICLLLFTSLLTLTSLSQVKPTPAGERMQSLDQRRKLK
                     AISLLNEVPFRNIGPTIMSGRVVDLEVNPDDPTEFYVAYATGGLWHTTNNGQSFTPIM
                     DSLDMLFIGDIAVKWETKTVQKGISVSDRIIWVGTGEVNSSRSSYAGVGVYKTNNNGK
                     VWEYVGLPESHHIGKIQLHPTNPDIAWVAVLGHLYSANPERGVYKTTNGGKTWKQTLY
                     IDDNTGCVELEI"
     gene            1381..2097
                     /locus_tag="PK977_15125"
     CDS             1381..2097
                     /locus_tag="PK977_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19506.1"
                     /translation="MRLLLVFVLFISISGYGQWKSYIIGVKGDTLNRVDMKGLKQGPW
                     AIKVPELRGERGYEEEGYFENDLKEGIWRKFAENGVKIAEETYRWGKLHGKQYYFTYN
                     GGLVREESWRAIDPANAYDTVPVYDLKDPSKILTMAIVKNDGVAMKHGKWLYYDPREG
                     TVEASEVYVMNKLQTDDGSMFNDDEIRPIAISQGKQKSDTAAVKTTQKPQVILDYEKK
                     NSGKKKIKVRDGSTGNLPKP"
     gene            complement(2108..>2846)
                     /locus_tag="PK977_15130"
     CDS             complement(2108..>2846)
                     /locus_tag="PK977_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRF19507.1"
                     /translation="AMKKEYKCVVIKPAAAQNENQSFPVVYLLHGHSGWYANWLIRVP
                     ELKDYADQYKLMIVCPDGGFNSWYMDSPLDSTVRFETYISEEVPAYIEKHYPVIKDRK
                     ARAVTGLSMGGHGGLFLAFRNAEVFGACGSMSGGVDLRPFPKSWQLSRLLGDTLQHAD
                     NWNNYSTINVIEKKPSDSLAIIIDCGTEDFFYAVNKALHEKMLQLKIAHDYIERPGKH
                     DWNYWRNAVKYQLLYFREYFQKMNSLK"
     assembly_gap    2847..2911
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3022..>3763)
                     /locus_tag="PK977_15135"
     CDS             complement(3022..>3763)
                     /locus_tag="PK977_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HRF19508.1"
                     /translation="FRTLEDASGEDLDWFWRGWFYSTEVCDISLDTVKHAKPDLSAVA
                     PETKETVVTRSLEKPQVPTFDDISKIRNREDKNITFQTDADTSLRDFYWRYARGIEPY
                     DTTKYKVTIPGNKPESLTAEEKEKYGNKNMYELTFSNKGGLVMPIIVEWTYKDGTKEI
                     DRIPAQVWRLNENKVIKTFIKDKEVASVKLDPYRETADINESNNSWNTIAEPSRFSIF
                     KAGMGGGRRGGGGQGGNPMQKAQEKKGF"
     assembly_gap    3764..3778
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3779..4688)
                     /locus_tag="PK977_15140"
     CDS             complement(<3779..4688)
                     /locus_tag="PK977_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF19509.1"
                     /translation="MTVPADHVIMSTGECQNYAAVLTPAQMARWKKAQTATEPVEVVT
                     LDEAKAAEKNGPAMIASASPKKKTWIFKADNVRDFAWGSSRKFIWDAMATKVEGKKIM
                     CMSGYPKEAYGLYRKFSTKAVEHTIKTYSQFTIPYPYPVAQSIEAANGMEYPMICFNF
                     GRTEKDGTYSEGTKNGMIGVIIHEVGHNFFPMIINSDERQWSWMDEGLNTFVEYLAEE
                     LWDNKFPSRRGPAAYITDYMKLPKEQLEPIMTNSENIVQFGPNAYSKPATGLNILRET
                     IMGRELFDYAFKEYARRWAFKHPEPAD"
BASE COUNT         1168 a         1038 c         1056 g         1345 t
ORIGIN      
        1 cagaaacata gatcttggca aaataataac cgtaagtgct gaagatgttg atgggtttct
       61 ggttggtctt tttccaggtg gcaccgccgt cttcgctttt atacacttcg cagccggcaa
      121 tgccgctgtt ctgaaaacca tcgtcactgt ccaggtaatc ccaaagggca gtgggcggta
      181 ctttgtcagc agccaccatc tctttcacca tcttnnnnnn nnnnnnnnnn nnnatacttc
      241 cggggaaatc cgttcttgcg aaggaaagtg tcgatcaggt ttgtcttcag cgctgcaaat
      301 tgttctttag tgatgttttt aaaatccgct tttttataaa atgtatctac cttcttcgat
      361 gtatcgggtt gcggcgtgtt attgtcaacc actgcccata cgatgttggg attattcggg
      421 taaacagcca ggccaatgcg gccgatcttg tcgcctgtta caaagccgga acccgggccg
      481 gatattaatt tccatgtttc gccgccatcc gtactcttat agatgccact ggtctttccg
      541 ctttcttcaa atttccaggc acggcgggtg cggtaccaca taccggcata gagttcattn
      601 nnnnnnnnnn nnnnnnngat ttctaattct acgcaacctg tattatcgtc tatgtataaa
      661 gtttgtttcc atgtcttgcc gccattggtg gttttgtaaa cacctctctc cgggttggca
      721 gaatacaggt gacccaatac ggctacccat gcaatgtccg ggttcgtggg gtgcaactgt
      781 atcttaccga tatgatgtga ttcgggcagg cccacatact cccatacttt accattattg
      841 ttcgttttgt aaacacctac acctgcatag gaggatctgc tgctgtttac ctcaccggta
      901 cctacccaga tgatacggtc ggaaacgcta attccttttt gtaccgtttt tgtttcccat
      961 tttaccgcta tatcaccgat gaagagcata tcaaggctgt ccataatggg tgtaaagctc
     1021 tggccattat tggtggtatg ccaaagaccg ccggtagcat aggccacata gaattcagtc
     1081 gggtcatccg ggtttacttc caggtcaacc acacggccac tcatgatagt agggccaata
     1141 ttgcgaaaag ggacctcatt aagcaacgaa atggctttga gtttgcgtcg ttgatcgaga
     1201 gactgcatcc tttcaccggc cggtgtgggt tttacctgcg acaggcttgt taaagtaagc
     1261 agtgatgtaa aaagcaggag gcaaatttgc cctaattttg ataccataat cagttgtttt
     1321 ggcgttaaag cggagaaggt taatggcagc agcgatagct tctttcaaac ttactaaact
     1381 atgcgtttac ttcttgtctt tgttttattt atttccatca gcggttacgg ccagtggaaa
     1441 agctatatca ttggtgtaaa gggtgataca ctgaaccgtg tggatatgaa aggcctgaag
     1501 cagggaccct gggccattaa ggtgcccgaa ttgcggggag aacggggata cgaggaagaa
     1561 ggttattttg aaaatgacct gaaagaaggg atatggagaa aatttgccga gaacggggtt
     1621 aaaatagcag aagaaaccta tcgctggggt aaactgcatg gcaagcagta ttattttact
     1681 tacaacggcg gcttagtgcg ggaagaaagc tggcgggcca ttgacccggc caatgcctat
     1741 gataccgttc cggtgtacga cctgaaagat ccttccaaga tactaacgat ggcaattgtg
     1801 aaaaatgacg gtgtggccat gaagcatggc aaatggttat actacgaccc gagagaggga
     1861 accgtggagg cctcagaagt atatgttatg aataaattac aaacagatga tggctcaatg
     1921 ttcaacgatg atgaaataag accaatcgcc atcagccagg gaaaacaaaa atcagataca
     1981 gctgccgtaa aaaccacgca gaagccccag gtaatactgg actatgaaaa aaagaactcc
     2041 ggtaaaaaga agatcaaagt gagagacggc agtacgggga atttgcccaa gccttagtta
     2101 ttcctattta cttaagtgaa ttcatttttt gaaaatactc cctgaagtaa agcagctggt
     2161 atttcaccgc attacgccaa tagttccagt catgtttgcc cggccgttca atataatcat
     2221 gggcaatctt tagttgcagc attttttcat gcaaggcttt attcacggca taaaagaaat
     2281 cttctgttcc gcaatcaata atgatagcca gtgaatcgga tggcttcttt tcaatgacat
     2341 taatagttga atagttattc cagttatccg catgttgtaa agtatcaccc aacagccggg
     2401 agagctgcca gcttttggga aaaggccgca ggtccactcc gccactcata ctgccgcagg
     2461 cgccaaacac ttctgcatta cggaaggcga gaaataatcc gccatgcccg cccatgctaa
     2521 gcccggtaac cgcccttgcc ttccggtctt tgataaccgg gtaatgtttt tcaatatagg
     2581 cagggacttc ttcacttata taagtttcaa atctgacagt tgaatctaac gggctgtcca
     2641 tataccagct gttgaagccg ccatcggggc aaacgatcat cagcttgtac tgatctgcat
     2701 aatctttcag ctcaggcaca cgaataagcc agttggcata ccagccgcta tgtccatgca
     2761 gtaaataaac aacaggaaac gattggtttt cattttgcgc tgctgcaggt tttatcacca
     2821 cacatttgta ttcttttttc atggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagagaatt ccgatcaaca atacaaagta
     2941 tctcttcatt gattaaagat ataaaaagag aagcacccgt tttgcggatg cttctgcctt
     3001 aataactaat taacttgttc actagaatcc tttcttctcc tgtgcttttt gcatggggtt
     3061 gccaccctgg ccaccgccgc ctcttctgcc accgcccatt ccggctttga aaatagagaa
     3121 gcgggaaggt tcagcaatcg tattccagct gttattgctt tcattgatgt cggctgtttc
     3181 cctgtagggg tccaatttta cggaggcaac ttctttatcc ttgataaaag ttttgatcac
     3241 tttgttttcg ttgagcctcc aaacctgtgc aggtatcctg tcgatttctt tcgtaccatc
     3301 tttgtaagtc cactctacga tgatcggcat taccagtccg cccttgttgc tgaaggtaag
     3361 ctcatacata tttttattgc catacttttc tttttcttca gcagtaaggc tttcgggttt
     3421 gttgccggga atagtcactt tgtatttggt agtatcataa ggctcgattc ccctggcata
     3481 tcgccagtag aaatcacgga gcgatgtatc tgcatccgtc tggaaagtga tattcttgtc
     3541 ttccctgttg cggatcttag agatatcatc aaaggtgggc acttgtggtt tttccaaact
     3601 acgggttact actgtttctt ttgtttcggg agccacagca gacaggtcag gcttggcatg
     3661 ctttacggta tctaaagaaa tatcgcatac ttctgtgctg taaaaccatc ctctccagaa
     3721 ccaatcaagg tcttcaccgc tggcatcttc cagcgtacgg aagnnnnnnn nnnnnnnnag
     3781 tcagccggtt ccggatgttt gaaagcccat cttcttgcat attctttgaa tgcataatca
     3841 aataactcac ggcccataat ggtttctctc aggatattta aaccggtagc tggttttgaa
     3901 taggcgttgg ggccaaactg aacaatgttt tcactgttgg tcatgatagg ctccagttgc
     3961 tctttcggaa gcttcatgta gtcggtaata taagcagcag ggccacggcg gctggggaat
     4021 ttattatccc agagttcttc ggccaggtat tcaacaaatg tattcaggcc ttcatccatc
     4081 cagctccact gacgttcatc actgttgatg atcatgggga agaagttgtg gccaacttca
     4141 tggatgatca caccgatcat gccgttctta gttccttcgc tgtaggtgcc atctttttct
     4201 gtgcggccaa agttgaaaca gatcatgggg tattccatgc cattggcagc ttcaatactt
     4261 tgcgcaacag ggtaggggta agggatagtg aattgggaat aagttttaat ggtatgctca
     4321 accgccttag tagaaaattt tctgtaaagg ccataggctt ctttgggata tccgctcata
     4381 cacatgatct ttttaccttc tactttggtg gccatggcat cccagatgaa tttgcgggaa
     4441 gagccccagg caaagtcacg gacattgtct gctttgaaaa tccatgtttt ctttttgggt
     4501 gaagccgaag caatcattgc cggtccattt ttttcagcgg cttttgcttc atcaagcgtt
     4561 accacttcaa cgggctctgt agcggtctgt gcttttttcc agcgggccat ttgtgccggt
     4621 gttaatactg cagcataatt ctggcattcg ccggttgaca tgatcacatg gtcggcaggt
     4681 acggtcatct gtactttgaa gttgccgaat gtaagtgcaa attc
//
LOCUS       DAORLS010000238         4697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_9991_length_4697_cov_2.016803, whole genome shotgun sequence.
ACCESSION   DAORLS010000238 DAORLS010000000
VERSION     DAORLS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9991_length_4697_cov_2.016803"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3272)
                     /locus_tag="PK977_15145"
     CDS             complement(<1..>3272)
                     /locus_tag="PK977_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRF19510.1"
                     /translation="FLIITHPALLNGSGGSTPVEDYKAYRNSAAGGSHNAKVYMIDQL
                     VDQFGFGIKQHPLSVRNFIRWARNTYTSPVKNVFLVGKGLNYIHNHFFQNNPDLDKLS
                     FVPTFGYPSSDNLLAADPGLNVIPKVPIGRLSVINGDEITDYLNKVKEYEQKQAISSP
                     VIADKAWMKNVVHVIGANDGALGDALTASMNRFSDIIKDTFYGGHVSTFSKLSSAPVE
                     QASSTRLYNLFEEGIGIMTYFGHSSATTLEFNLDNPVNYNNRGKYPLFILLGCNAGNF
                     YMFNTLRLQTKETISEKYVLAPQRGSIGTIASTHLGIVHYLDIYNSKNYSSFSVNKYS
                     GTMGEAMVDAITQTFNLTTQNDFYARFHCEQTTLHGDPAVHLNGFAKPDYVIEDNLVK
                     LDPSFISVAETNFRVDAKFMNIGKATSGNIVVEVKRTYPNNSTEVIKRDTIPGVRYID
                     SLSYVIPIVATRDKGLNKITITLDADNIVDELYESNNTITKEFFIYENEARTVYPYDY
                     AIVNRQDIKLFVSTANPFAVLQEYRIEIDTTALFNSSLKVSRTFNSVGGLLSFDPGIT
                     FVDSTVYYWRVSPTPAAGERKWNGSSFVYLPNSDEGFNQSHFYQHTKSEFQRMKLDSA
                     SRKLQYGEVMNNVFLRMGTYNTSGSTQESSLIAAINGFQISRLTNWFSSLVFNVIHPV
                     TFKPWQNQVVVPHSYVGEPANPNSLGQGLYGSTSPSFINTRPYSFEFRYTDTASRRKI
                     MNFMRDSIPDGYYVIVRNFTLYPAAYPAFPVAYIADWAADESLYGQGQSVYHYLKAAG
                     FAGIDSFYRPRPFGFVYRKNVPSFTPSWIVGDGVFDNPTLSVECPSPDTLGYIYSPVF
                     GQAKRWKQLFWRGSILPDITPGDDPSVDIIGVRHNGTESVIINNLSIAQQDYDISSID
                     ADEYPYLRLRMRNVDSIHYTPYQLNYWRLTYDPVPEGAVAPNIYFQHKDTLDVGEPFN
                     FSVAFKNISKVPFDSLKVKMVVTDRNNVANIIPLPRKKPLGAFGSSTDSVHIGSIINT
                     SAFPGKNTLFLEANPDNDQPEQHHFNNFTFRDFYVKPDSLSPVLDVTFDGVHILNRDI
                     VS"
     assembly_gap    3273..3282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3283..4472)
                     /locus_tag="PK977_15150"
     CDS             complement(<3283..4472)
                     /locus_tag="PK977_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19511.1"
                     /translation="MKRIILPLLLFTGLFVQGQSYNNEWIDYSKTYYKFKVGADGLYR
                     INQSVLATAGLGSVPAENFQLWRNGQQIPLYTSVQTGVFGISDYIEFWGEMNDGKPDL
                     PLYRIPDHQLNDKWSLETDTAFYFLTVNPSGTNLRLVPTTNNLPTSLTPEPFFIHTVG
                     KYYRDKYHFGYAAVVGEYVYSSNYENGEGFTSPNLAANATRTETFSNLFPSSNAGAPS
                     PFLRINATGDALNPRQFEVRVNGNLVATQTMDYFDSVKANIPISITDISSGTAALAVK
                     NISPGASDRMVIAKSELVYAREFKFGGANNFSFELPANAAGNYLEITGFNYGTLPPIL
                     YDLTNGKRYVADIINAPTIRVVLEPSATSRKLLMVTQDAAYPRQVTSLQQRNFINYAL
                     PANQG"
     gene            complement(4499..>4697)
                     /locus_tag="PK977_15155"
     CDS             complement(4499..>4697)
                     /locus_tag="PK977_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19512.1"
                     /translation="ALSDGDTVNQKKVWIYQPSAGAGFKIQNVTIDYAYTNLANQSNP
                     LFTHVFSLRLNLVNNNKKKED"
BASE COUNT         1451 a          951 c          856 g         1429 t
ORIGIN      
        1 agaaacgata tccctattaa gtatgtgaac cccatcgaaa gtaacatcca gcactgggct
       61 taaactatct ggctttacat aaaagtctct aaaagtaaaa ttattaaaat gatgttgctc
      121 aggttgatca ttatccgggt tggcttccag gaacagagta ttctttccag gaaatgcaga
      181 tgtatttatt attgagccaa tatgaaccga atctgttgaa ctaccaaatg ctcccaatgg
      241 ctttttgcga ggtagcggga taatattggc aacattgttc ctatcggtga caaccatttt
      301 aacctttaag ctatcaaagg gaaccttgct gatattctta aaagcaacgc tgaagttaaa
      361 tggctcgcca acgtcaagag tgtctttatg ctgaaaatag atgtttggag caactgcacc
      421 ttcaggtact ggatcatatg ttaaccgcca ataatttagt tggtatggtg tgtaatgaat
      481 tgaatcaaca ttcctcattc ttaatcgcaa atacgggtat tcgtctgcat caatcgatga
      541 tatatcataa tcttgttgtg caatagaaag attatttata ataacggact ctgtcccatt
      601 atgcctgact ccaataatat caacacttgg gtcatcgcca ggtgttatat ccggtaagat
      661 agatcctctc caaaataatt gtttccaacg ttttgcctga ccaaagacag gagagtaaat
      721 atatcctaat gtatcaggcg aaggacactc aacacttaaa gttggattat caaatactcc
      781 atcccctact atccaacttg gtgtaaaaga cggaacgttt ttccgataga caaatccaaa
      841 tggccgtgga cggtaaaacg aatctatacc tgcaaatccc gcagctttta gataatggta
      901 cacagattgt ccctgaccat acaatgactc atcggcagcc cagtctgcta tatatgccac
      961 agggaatgcc ggatacgcag caggatataa ggtaaaattc cttacaatta catagtaccc
     1021 atcaggaata ctatctctca taaaattcat tattttcctt ctgctggcag tatctgtgta
     1081 tctgaattca aaactataag gccttgtatt aataaatgaa ggagatgtac taccatacaa
     1141 tccttgaccc agtgaatttg gattagccgg ttcaccaaca taagaatgag ggacaactac
     1201 ctggttttgc catggtttga atgtaaccgg gtgaattaca ttaaaaacta aagaagaaaa
     1261 ccagtttgtc aatctagaga tttggaatcc attaatagcg gctattaaag acgactcttg
     1321 tgttgaaccg ctggtattat aagttcccat acggagaaat acgttattca ttacttcgcc
     1381 atattgcaat ttccttgaag cactgtccag cttcattctt tggaactcag acttcgtatg
     1441 ctgataaaag tgcgactgat taaacccttc atcgctatta ggtagataca caaatgaaga
     1501 cccattccac tttctctcac ctgctgcagg agtcggagat acccgccaat agtaaacggt
     1561 gctgtcgaca aaagtaattc cgggatcaaa agataaaagt ccgcctactg aattaaaagt
     1621 ccttgaaact ttaagggacg aattaaataa tgcagttgta tcaatctcga tcctatattc
     1681 ctgcaaaacg gcgaagggat ttgctgttga aacaaataat tttatatcct gacgattaac
     1741 tatagcataa tcataaggat aaactgtcct tgcttcattc tcgtaaataa aaaattcctt
     1801 tgtaattgta ttattggatt cgtacagctc atcgacaata ttatctgcat ctaatgtgat
     1861 ggtaatttta ttgagtcctt tatcacgggt tgccacaata ggaattacat agctcaatga
     1921 gtctatatac ctgaccccgg gaatagtatc tcttttaata acctctgtag aattattggg
     1981 ataagtacgt ttaacttcta caacgatatt tccgctggtg gcctttccaa tattcataaa
     2041 ttttgcatca accctaaagt ttgtttctgc cacagatatg aaagaaggat ccagcttaac
     2101 caaattatcc tctattacat aatcgggctt ggcaaatcca tttaaatgaa cagccggatc
     2161 tccatgtaat gttgtttgct cacaatgaaa tctcgcatag aaatcattct gtgtagtcag
     2221 attaaacgtt tgggtaatgg catcaaccat tgcctcaccc attgttccac tgtacttatt
     2281 tacactaaat gaagaatagt tttttgaatt gtatatatcc aaataatgta caattcccag
     2341 gtgagtactc gcaatcgtac ctatactacc tctctgcggt gccagtacat atttttcaga
     2401 tatagtttcc tttgtctgta accttagcgt attaaacatg tagaaattac cagcgttaca
     2461 tcctaacaat atgaataatg gatacttacc tctattgttg taattcactg ggttatcaag
     2521 attaaactca agagtagtgg ctgaagaatg accaaaatat gtcataatac ctatcccctc
     2581 ttcaaacaag ttgtataacc ttgtagaact tgcctgttca actggtgcag atgaaagttt
     2641 actaaaagta cttacatgtc ctccataaaa ggtatcctta ataatgtctg aaaatctatt
     2701 cattgaagct gtcaaagcat cgcccaatgc accatcattg gcacctataa catgaacaac
     2761 atttttcatc caggctttat cggctataac aggagacgaa atagcctgct tctgttcata
     2821 ttcctttacc ttgttaagat aatcagtaat ctcgtcacca ttaatcactg ataaccggcc
     2881 tatggggact ttgggtatta cgttcaagcc cggatcagca gcaagaaggt tatcggaaga
     2941 aggatatcca aatgttggta caaatgacaa cttatccaaa tcaggattat tttgaaaaaa
     3001 atgattatga atataattta aacctttccc tactaaaaaa acattcttta ctggtgaagt
     3061 atatgtattt cgtgcccagc gaataaagtt ccgtacagat aaaggatgtt gctttatacc
     3121 aaatccaaac tgatcaacta attgatcaat catatagact ttcgcattat gacttccgcc
     3181 agcagcagaa ttacgatagg ctttgtaatc ctctacagga gttgacccgc ctgagccatt
     3241 taataaggcc ggatgagtaa tgattagaaa gtnnnnnnnn nnccctggtt tgcaggaagg
     3301 gcatagttta taaaatttct ctgctgcaaa ctggttactt gccttggata agcggcatcc
     3361 tgtgttacca ttaacaattt cctggatgtt gcagaaggct caagtaccac tcttattgtt
     3421 ggcgcattaa taatatccgc cacataacgt tttccgtttg taaggtcata aagtattgga
     3481 ggcagggttc cgtagttaaa cccagtaatc tccaggtaat ttcctgcagc attagctggt
     3541 aattcaaaag aaaaattatt tgccccgcca aatttaaact ctcttgcata aacaagttct
     3601 gatttggcaa tcaccatcct gtcgctggct cctggggaaa tattcttaac cgcaagggca
     3661 gccgtgccgg agcttatatc agttatacta atgggtatat tagccttaac agaatcaaag
     3721 taatccattg tttgtgttgc aacaaggttt ccattcactc ttacttcaaa ttgtcttgga
     3781 ttcagcgcat ctcctgttgc atttattctt agaaagggag atggcgcccc tgcatttgaa
     3841 gatgggaaaa gattactgaa tgtctctgtc cttgtagcgt tggctgcaag gtttggcgat
     3901 gtaaatcctt ctccgttctc ataatttgag gaataaacat actctcctac cacggcggca
     3961 taaccaaaat gatatttatc cctataatat tttccgactg tatgaataaa gaaaggctca
     4021 ggtgttaatg atgtgggcag attattagtt gtcggaacta accgtaggtt tgtgccagat
     4081 ggattcacag taagaaagta aaaagctgtg tcagtctcca aactccattt atcattcagc
     4141 tgatgatcgg gaattctgta taaaggcaaa tcaggttttc catcattcat ttcgccccaa
     4201 aactcaatat aatctgaaat gccaaataca ccggtttgaa ctgaggtgta aagaggaatc
     4261 tgctgcccat tcctccataa ttgaaaattt tcggctggca cactgcctaa cccggccgta
     4321 gccagaacag attgatttat ccggtataat ccgtcagcac ctactttaaa cttatagtag
     4381 gtcttgctat aatcaatcca ctcgttatta tacgactgac cctgaacaaa gagtcctgta
     4441 aagagcagca atgggagtat aattcttttc atttcaataa gtgtttatcg ggaacagatt
     4501 aatcttcttt tttcttattg ttattaacca ggttcaaccg caaggagaaa acatgggtaa
     4561 acaaagggtt ggattggttg gccaggttgg tatacgcata atcaatcgta acattctgga
     4621 ttttaaaccc tgcaccagcg ctgggttgat aaatccaaac cttcttttga tttactgtat
     4681 ctccatcgga taatgcc
//
LOCUS       DAORLS010000239         4685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10024_length_4685_cov_1.629158, whole genome shotgun sequence.
ACCESSION   DAORLS010000239 DAORLS010000000
VERSION     DAORLS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10024_length_4685_cov_1.629158"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..>818
                     /locus_tag="PK977_15160"
     CDS             117..>818
                     /locus_tag="PK977_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19513.1"
                     /translation="MPYCRWVLLLLFSTGFSANAQKIVYSEPDKEDSRRMNFEIAGKI
                     NGNFLIYKNVRNKNWIVVLDNEMQQIAKVEQDYVPDNDRMINADFFPYQDFCYMIYQY
                     QKKNVVYCVASKIDGMGNKVGDVIELDTTHIGFAANNKIYTVLSSEDKSRLSVFKINS
                     RNRKVYIMTTMLMNDKLELLKKSRLSIPMEERNEFLGDFNLDNEGDLVFSKFERTNND
                     NISRAFFVIKHAQADT"
     assembly_gap    819..832
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <833..>1647
                     /locus_tag="PK977_15165"
     CDS             <833..>1647
                     /locus_tag="PK977_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19514.1"
                     /translation="MAKELNIEKTWLDEIHIKVDNPNKRYLLTSFYYKERRGNVDGFY
                     FYVWDKTTEQPIMEAIHTFTDEFRKDARGDASVKMAFNDYFIRNIITRKDGGFIIAGE
                     AYYTTSRFNNWNRWDYLYGSPYFGGLNSWYYSPYYRNMWWGSRMNTNQAVRYHADNIA
                     VLSFDNKGQLEWDNVIGKSQFDDESDDRVSYQVMNTGGQLHFLFNQQERRNNLLTDFS
                     LTPAGELTRNPTLKNLDKGYEFMPKYGKQVSARQMIVPCIYRNYICFAKIDYT"
     assembly_gap    1648..1803
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1804..>2426
                     /locus_tag="PK977_15170"
     CDS             <1804..>2426
                     /locus_tag="PK977_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HRF19515.1"
                     /translation="GLLYREIIHFLTPAGKANPVIYPLLAVGLLFLQAVILTRFINHQ
                     RMTSHQTWYPGMAYLLLTSLFPEWNYFSAPLIINTFLLFILSALFKTYNQSDARGSIF
                     NIGLALGMGSFVFFPSVTFIIWVILALAVMRPFRLSEWLLCMVGATTPYYFFAVYLFI
                     TDQWSWQNLWPHFSIDLPVLLPSLWLAGGAFLLVMPFLAGGYFVQDNL"
     assembly_gap    2427..2471
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2472..2685
                     /locus_tag="PK977_15175"
     CDS             <2472..2685
                     /locus_tag="PK977_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19516.1"
                     /translation="SLLLLYLLGALFVPFINSTNSLENWVIVAIPFAAFHASMYIYAS
                     FRIVPQLFFWLSVGFILTYQYWGPGW"
     gene            2749..3417
                     /gene="purQ"
                     /locus_tag="PK977_15180"
     CDS             2749..3417
                     /gene="purQ"
                     /locus_tag="PK977_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRF19517.1"
                     /translation="MKFGVVVFPGSNCDRDMHDALKYDLGQDVTMLWHKDRDLSKFNT
                     EDCIILPGGFSYGDYLRCGAIARFSPMMQSVIGFANAGGKVFGVCNGFQILCESHLLP
                     GALLRNANQQFVCKNVYITNETGKPYKIPIAHGEGRYYADEKTLNELEANGQVIFYYC
                     DENGKITAEANPNGSTRNIAGICNAERNVFGMMPHPERACSGVLGNYKGNADGRASEC
                     WLKK"
     gene            complement(3466..>3706)
                     /locus_tag="PK977_15185"
     CDS             complement(3466..>3706)
                     /locus_tag="PK977_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19518.1"
                     /translation="KEEKYYINKKTYLIDKVVILTTNIEPRKGGGYFETFANYEDYRN
                     VNGLMIPFKVNQANIITIKVQNAEVKPIDDKIYEN"
     assembly_gap    3707..3735
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3736..4064)
                     /locus_tag="PK977_15190"
     CDS             complement(<3736..4064)
                     /locus_tag="PK977_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19519.1"
                     /translation="MLTYRQSVVAFNTNGKIYYSYPDKFKNTNQFEETEQMLMLLGKD
                     YFTEKYAIMNGEKVSTNNPAQFNLKDSWISKLNGLELFLHKTINNATVKFAEENTTTT
                     DSCYVLK"
     assembly_gap    4135..4154
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4155..4576)
                     /locus_tag="PK977_15195"
     CDS             complement(<4155..4576)
                     /locus_tag="PK977_15195"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF19520.1"
                     /translation="MNAVRKKAFDFLLQDSTGKDYRLADFKGKMLFIDFWASWCAPCK
                     AQIPHQKELEKAYAGKDIVFASVSLDKSKPAWLKAVRDEDLHGTILHAKGDFKNEFPK
                     AYGIESIPRYMLIDADGYIISDNMMKPENKKEIKGIID"
BASE COUNT         1217 a          821 c          925 g         1458 t
ORIGIN      
        1 agggaataaa aattgcatgg ctcatgtaaa atgctaaaaa atggcatcag tataaaatac
       61 tcggaccggt tttggagaac tacttgtaac tttaatcagc aaaaaatcag attattatgc
      121 cttactgtag atgggtgctg ttgttgcttt ttagtaccgg attttctgct aatgcccaga
      181 aaattgttta ttctgaacca gacaaggaag attcaaggag gatgaatttt gagatagccg
      241 gtaagatcaa cggcaatttc ctgatttata aaaatgtcag gaataagaac tggatagtag
      301 tgctggataa tgaaatgcag cagatagcca aggtggagca ggactatgta cctgataatg
      361 acaggatgat caatgctgat ttttttcctt atcaggattt ttgctacatg atttaccagt
      421 accagaaaaa gaatgtggtt tactgtgttg cttcaaagat tgatggcatg ggtaataagg
      481 taggagatgt tattgaactg gataccactc atatcggttt tgccgccaat aataaaatct
      541 ataccgtttt aagcagtgaa gacaaaagcc ggctatctgt ttttaagatt aatagccgca
      601 accggaaagt atatataatg accaccatgc tgatgaatga caagctggaa ttgctgaaga
      661 aaagccgcct gtcaattccc atggaggaga ggaatgagtt tttgggcgac ttcaacctgg
      721 ataatgaagg agacctggta ttcagcaaat ttgaaagaac aaataatgat aacatcagca
      781 gggctttttt tgtaataaaa catgctcagg ccgataccnn nnnnnnnnnn nngatggcaa
      841 aagagctcaa cattgagaag acctggctcg atgagataca catcaaagtg gataatccca
      901 ataagcggta tcttcttact tcattttatt acaaagaaag aaggggtaac gttgacggtt
      961 tttattttta tgtgtgggac aaaacaacag aacaacccat aatggaagct attcacactt
     1021 ttactgatga attccgaaaa gatgcccgtg gagatgccag tgtgaagatg gcttttaatg
     1081 actattttat tagaaacatt attacccgta aagacggcgg atttatcatt gccggtgagg
     1141 cgtactatac tacatcccgt ttcaataact ggaaccggtg ggattacctc tatggctcac
     1201 catatttcgg gggacttaat agctggtact actctcctta ctatcgaaat atgtggtggg
     1261 gaagccgcat gaacaccaac caggcggttc gctatcatgc cgataatatt gctgtactgt
     1321 ctttcgataa taaaggtcag ttagaatggg ataatgtgat tggaaaaagc cagttcgacg
     1381 atgagtctga tgaccgtgtt tcttaccagg taatgaatac aggagggcaa ctgcattttc
     1441 tcttcaacca gcaggaacgt agaaataatt tattgacaga ctttagtctt actcctgccg
     1501 gtgaactgac ccgtaatcca actttaaaga accttgataa aggctatgag tttatgccga
     1561 aatatggaaa acaggtaagt gcccgtcaaa tgattgttcc ctgtatctat cgtaactata
     1621 tttgctttgc caaaatagat tacacacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnggtttat tgtatcggga gatcattcac tttcttactc ctgccggtaa ggccaatccc
     1861 gttatatacc ctttgctggc tgttggatta ttgtttcttc aggccgttat ccttacaagg
     1921 tttatcaatc atcaacgtat gacaagccat caaacctggt atcccggaat ggcttacctg
     1981 ttgctgactt ccttgtttcc tgaatggaac tatttttcag caccacttat cattaatacc
     2041 tttttacttt tcattttatc agctttattc aaaacctaca accagtccga tgcaaggggc
     2101 agtattttta atattggcct tgccttaggt atggggtctt tcgttttttt cccttctgtc
     2161 actttcatca tttgggttat tttggcgctg gcagtgatgc ggcccttcag gttgtcagaa
     2221 tggttattat gtatggttgg cgctaccact ccctattatt tctttgctgt ttatcttttt
     2281 ataactgatc aatggagctg gcagaacctg tggcctcact ttagtataga tttacccgtt
     2341 ttactcccat ccttatggct ggctggcgga gcttttttgt tggtaatgcc tttcctggca
     2401 ggtggttatt ttgtgcagga caacctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn ngagcttgtt gttattatac ctgctggggg ccctttttgt accttttatt
     2521 aatagtacaa acagccttga gaattgggtg attgttgcca taccttttgc cgcttttcat
     2581 gccagcatgt acatatacgc ctcattccgg attgtgcccc agcttttttt ctggttatct
     2641 gttggtttta tccttactta ccaatactgg ggaccgggtt ggtaaaaggt ggaaaagagt
     2701 tgaactgccg gggttatctt tgttccttaa ttttaatatt catcatttat gaaatttggc
     2761 gttgtagttt ttccgggatc taattgcgac agggatatgc atgatgccct gaagtacgac
     2821 ctggggcagg atgttaccat gctctggcat aaggacaggg acctcagcaa attcaataca
     2881 gaagattgca taatcctgcc gggcggtttt tcatacggtg attatttacg ttgcggggcg
     2941 attgcccgtt ttagcccgat gatgcaaagt gtgatagggt ttgccaatgc cggaggaaag
     3001 gtatttgggg tttgtaacgg cttccagata ctgtgtgaat cacatttgtt gccgggtgct
     3061 ttattaagga atgccaacca gcaatttgtc tgtaaaaacg tatatatcac aaacgaaacc
     3121 gggaaaccct ataaaattcc aattgcccac ggtgaaggtc gttactatgc tgatgaaaaa
     3181 acgttgaatg aattggaggc taatggacag gtaatttttt attactgtga tgagaatggg
     3241 aaaataactg ctgaagccaa ccccaacgga tctacccgca atattgccgg catctgtaat
     3301 gctgagagga atgtattcgg tatgatgcca catccggaac gagcctgttc gggtgttttg
     3361 ggaaattata agggcaatgc tgatggcaga gcaagtgaat gttggctaaa aaaatagaga
     3421 atgatcaggg tcactctcca ttttttgact taatatcttt tgtgtctaat tttcataaat
     3481 tttatcatcg attggtttca cctcagcatt ttgaactttt atagtaatta tatttgcctg
     3541 gttaactttg aagggtatca ttaacccgtt tacattacga tagtcttcat aatttgcaaa
     3601 tgtttcaaag taacctcctc cttttcgtgg ttcaatgttg gtggtcagaa tgactacttt
     3661 atcaataagg taggttttct tatttatata atatttctcc tctttannnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnatttt cagtacgtag caactgtctg tagttgttgt attttcttct
     3781 gcaaatttta ctgttgcatt gttaatagtt ttatgtaaaa aaagctccaa cccgttaagt
     3841 ttacttatcc atgagtcttt taagttaaac tgggcgggat tatttgttga aactttttct
     3901 ccattcataa ttgcatattt ttcggtaaaa taatctttgc ctaatagcat cagcatttgc
     3961 tctgtttctt caaactgatt ggtatttttg aatttgtcgg gataactata ataaatcttt
     4021 ccattcgtat taaaggctac tacagactgg cggtaagtta acattaagcc attttgcttg
     4081 agtgcttcgg ctcctatggc tttaaaatgt ttttgaagaa tctcttctgt gtttnnnnnn
     4141 nnnnnnnnnn nnnntcatca ataatgccct ttatctcttt cttgttttcc ggtttcatca
     4201 tgttgtccga gatgatatag ccatctgcat caataagcat ataccgggga atggactcaa
     4261 ttccataagc tttcggaaac tcattcttaa aatctccttt agcatgtaaa atagtcccat
     4321 gcaaatcctc atcccttacg gcctttagcc aggctggctt tgatttgtcc aaagaaacac
     4381 ttgcaaagac tatatccttg ccagcatagg ctttttcgag ttctttttgg tgggggattt
     4441 gtgctttgca gggggcacac caggaagccc aaaaatctat aaaaagcatt tttcctttga
     4501 aatcagctaa cctgtaatct ttacctgtcg agtcctgtaa taaaaaatca aatgcctttt
     4561 tgcggactgc attcaaatct ggaagcttgt tagctctcag gcgttcaatt ttttccgtat
     4621 atgtatttct tacagattga gtagagttgt catataagta ggatacaaac gcttcatttt
     4681 taagg
//
LOCUS       DAORLS010000240         4654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10106_length_4654_cov_2.163296, whole genome shotgun sequence.
ACCESSION   DAORLS010000240 DAORLS010000000
VERSION     DAORLS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10106_length_4654_cov_2.163296"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..794)
                     /locus_tag="PK977_15200"
     CDS             complement(69..794)
                     /locus_tag="PK977_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF19521.1"
                     /translation="MQAMIFAAGLGTRFKPWTDKHPKALALVNGKSLLQRNIEYLQQY
                     GIKDVMVNVHHFADQIEEAIAKNNGWGSNITISDERDFLLDTGGGLLKAKPFFAPGER
                     FITCNADILTDLDINKLLAFHEGNAPLISFGVSKRKTSRNFLFDGNGQLCGWRNDNTG
                     EEKISRHNKHLVSKAYSCVVVFEYAVFRLMEQAGFLGKFSLTDVYLELAKQHTILGYD
                     HTGDRWADVGKTESVAIAESLFL"
     gene            complement(794..>1614)
                     /locus_tag="PK977_15205"
     CDS             complement(794..>1614)
                     /locus_tag="PK977_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRF19522.1"
                     /translation="MKGAPQYDVASMLWQARANLPDEWKNNLLEDYMNAFEATIGQRI
                     DRTIFRSQYNGYVLIRLLQVLGAYGFRGLFERKAQFLTSIPLALRNLKEFFEHQSVGI
                     SVPEFRKVLDICVADEIIQQFTPTQATDKTPLVIKIHSFSYRKQLPQDESGNGGGFIF
                     DCRGILNPGRIESMKTQTGRDKEVKDFLEQQTKMPEFLNSVFDIVDITVEEYIKRDFE
                     SLMISFGCTGGQHRSVYAADAMARHLKNKFKVKIELKHIVQEEKNWQNPLPENK"
     assembly_gap    1615..1635
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1636..2271)
                     /locus_tag="PK977_15210"
     CDS             complement(<1636..2271)
                     /locus_tag="PK977_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19523.1"
                     /translation="MQHLIESISALYKQWKGTEPGSVDLLPQSGSERRYFRLHGKDGS
                     VIGTFGANVRENETFIYFSNHFRKNKLAVPEILAISEDKQFYLQEDFGDISLLNHLES
                     KGVTQEVYDLFKKSLEELARLQVKGDEGLDYNNCLTNKEFGKQAIMADLLYFKYYFLD
                     ALRKPYDKQKLIDDFEALSNYLTHTEYKYFMFRDFQSRNIMVTEDSAVHFID"
     gene            2365..>2944
                     /locus_tag="PK977_15215"
     CDS             2365..>2944
                     /locus_tag="PK977_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRF19524.1"
                     /translation="MSYNPTNKVRIVTAASLFDGHDAAINIMRRIMQSKGAEIIHLGH
                     NRSVKEIVETAIEEDVQGIAITSYQGGHVEFFKYMKDLLEENGCGHIKIFGGGGGTIL
                     PDEIDELHQYGIARIYSPDDGRKMGLEGMIEDVIRQCDVQLNGKADYTTPMKLGEVKD
                     VRVIARKITNAENGIEDGKKGNPSTAIPVLGIT"
     assembly_gap    2945..3064
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3065..>4654
                     /locus_tag="PK977_15220"
     CDS             <3065..>4654
                     /locus_tag="PK977_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF19525.1"
                     /translation="KKKTGGALLGDRIRMNAISHPRAYMRSLATRDDNTALSSAVQEA
                     IDICKAAGFDFVILESAGVGQSDASILDYCDVSMYVMTPEYGAASQLEKINMLDYADL
                     VCINKFDKAGALDALADVRKQYKRNHSLWTAKDEELPIIGTMASKFNDDGVNHLFELL
                     LKKIEAKTKVTFGNFHFAETAKVSQAVIPASRVRYLSEISENNRNYDQLVKDQAAIAS
                     KLYQLHGAKETLREKADKPLLDVVEQQISFYTEQLTPVARKLITNWPNKVTEYQKDFY
                     EYTVRDKVIKQPMTTKSLSGTRIPKVVLPKYNDWGDILKWQAQENVAGHFPFTAGVFP
                     LKREGEDPTRMFAGEGGPERTNKRFHYVSVDQPAKRLSTAFDSVTLYGEDPDYRPDIY
                     GKIGNSGVSIATVDDAKKLYSGFDLCDPKTSVSMTINGPAPMLLAFFMNAAIDQQCEK
                     WIMENGEGKMVADALKKKYPDGKIPVYNNPSFPGQLPEGNNGLGLKLLGLSGDEVLPA
                     AIYEQCKQSALQQVRGTVQADI"
BASE COUNT         1295 a          908 c         1031 g         1279 t
ORIGIN      
        1 atttgaaagc cgtaagataa tggaaatgac gggatgagta ctaccgttga aatggtattt
       61 gcattacttt ataaaaaaag tgattcagct atggccacac tttccgtttt gcctacatct
      121 gcccatctat cacctgtatg atcatagcca agaattgtat gttgtttggc cagttccagg
      181 tatacatcgg ttaacgagaa ctttcccaag aaaccggcct gttccattaa ccggaaaaca
      241 gcgtactcaa aaacaactac acaactataa gcttttgaaa caaggtgttt attatgccgg
      301 ctgatttttt cttccccggt attgtcattt cgccagccgc agagttgccc gtttccatca
      361 aaaaggaaat tgcgggatgt cttccttttt gataccccaa aagaaataag cggagcattg
      421 ccttcatgaa aagcaagtag cttatttata tcaaggtcag taagtatgtc agcattacag
      481 gttatgaatc tttccccggg agcaaaaaaa ggctttgcct tcagcagccc tccgccagta
      541 tcgagcagaa aatccctttc atcgctgata gtgatattac tgccccatcc gttgttttta
      601 gctattgctt cttcaatctg gtcagcaaaa tggtgcacat ttaccattac atctttgatg
      661 ccatattgct gcaggtattc tatgtttcgt tgtagcaagg atttgccatt taccaaagcc
      721 agtgcttttg gatgcttatc agtccatggt ttaaaccttg tgccgagtcc tgcggcaaaa
      781 atcattgctt gcattactta ttctccggca gaggattttg ccagtttttt tcctcctgaa
      841 caatatgttt caattcaatt tttactttga atttgttctt caaatgtctt gccattgcat
      901 ccgcggcata aacactccgg tgctgacctc ctgtgcagcc aaaactgatc atcaggcttt
      961 caaaatctct cttgatgtat tcctctacgg taatgtcgac gatatcaaac acactattga
     1021 gaaattcggg cattttggtt tgttgttcca gaaaatcttt cacttcttta tcacggcctg
     1081 tttgtgtttt catgctttcg atacggccgg gattcagaat gccgcggcaa tcgaaaatga
     1141 agccaccacc gttaccactt tcatcttgtg gcaattgttt tctgtaagag aagctgtgga
     1201 ttttaattac cagtggtgtt ttatctgttg cctgtgtagg cgtgaattgc tggatgattt
     1261 catcagccac acagatgtcc agcacttttc tgaattcggg aacggaaatg ccaacacttt
     1321 ggtgttcaaa aaactctttc aaatttctca gggcaagtgg tatgcttgtc aggaattggg
     1381 ctttacgttc aaataagcca cggaagccat aggctcccaa aacctgaagc agtcttatca
     1441 atacgtagcc gttatactgg ctacggaaaa tggtcctgtc tatacgctga cctatcgttg
     1501 cttcaaaggc attcatgtaa tcttccagca aattattctt ccattcatcc ggaagattgg
     1561 cccttgcctg ccagagcata gaggctacat cgtattgcgg ggcgcctttc atacnnnnnn
     1621 nnnnnnnnnn nnnnnatcga taaaatgaac ggcactgtct tccgtaacca tgatattcct
     1681 gctttgaaaa tcacggaaca tgaagtattt atattctgta tgagtcaggt aattgctcag
     1741 tgcttcaaaa tcatcgatca acttttgctt gtcatatggt ttacgtagtg catcgagaaa
     1801 gtagtatttg aagtaaagca ggtcagccat gatggcctgc tttccaaatt ccttattggt
     1861 caggcaattg ttgtaatcca gtccttcgtc tccttttacc tgcagacggg ccagttcttc
     1921 gaggcttttc ttaaagaggt catatacttc ttgtgtaact ccctttgatt ccaggtgatt
     1981 taataatgaa atatcaccaa agtcttcctg caggtaaaat tgtttgtctt cgctgatggc
     2041 aagaatttcc ggaacagcca gtttgttttt tctgaagtga ttggagaaat aaatgaaagt
     2101 ttcgttttct ctaacattgg cgccaaaagt tccgattaca gagccatctt taccatgcag
     2161 cctgaagtaa cgcctttcac tgccactttg cggaagcagg tcaacggaac caggttctgt
     2221 acccttccac tgtttatata gtgccgatat ggattcgata agatgctgca tgcggcaaat
     2281 gtagttaacc ccctgcaatc ccgcactttg gattggctaa caggcattag ttttttgggc
     2341 taaatttgct ttttcttaca acttatgagt tataacccta ccaataaggt tcgtattgtt
     2401 acggcagcca gtctttttga tggtcatgat gccgctatca atatcatgcg gcgaattatg
     2461 cagagtaaag gagctgagat cattcacctg ggccataacc ggtcagtaaa agaaatcgtt
     2521 gagacagcta tagaagaaga tgttcagggt attgctatta ccagttacca gggtggtcat
     2581 gtagagtttt tcaaatacat gaaagacctg ctggaagaaa acggatgtgg ccatattaaa
     2641 atattcggtg gcggtggcgg aactatttta cctgatgaga tagatgagct tcaccagtat
     2701 ggtattgccc ggatatatag tccggatgac ggaagaaaaa tgggattgga aggaatgatc
     2761 gaagatgtca tcaggcagtg tgatgtgcaa ttgaacggta aagctgatta cacaacacca
     2821 atgaaattag gtgaggtgaa ggatgtaaga gtaatagcca gaaaaattac taatgctgag
     2881 aacggtattg aagacggaaa aaagggaaat ccctcaaccg caattcctgt attaggaata
     2941 acggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnaagaaa aaaaccggag gtgctttgct gggcgaccgg attaggatga atgccatatc
     3121 gcatccaagg gcctatatgc ggagccttgc tacgagagac gataatacgg cactgagcag
     3181 tgcagtgcag gaggctattg atatttgtaa agcagccggt tttgatttcg ttattctgga
     3241 aagtgccggg gtaggacaaa gtgatgccag tattttagac tattgtgatg ttagtatgta
     3301 tgtgatgaca ccggaatatg gtgctgcctc acaattggag aaaataaaca tgctggacta
     3361 tgcagacctt gtctgcatca acaaatttga caaggcgggt gcattagatg cattggcaga
     3421 tgtgagaaaa caatacaaac gtaaccacag cctctggacg gctaaagatg aagaattacc
     3481 catcatcggc acgatggcca gcaagttcaa cgatgatggt gtgaaccacc tgtttgaatt
     3541 attactgaag aaaattgaag cgaaaacaaa ggtcaccttt ggtaattttc attttgcgga
     3601 aactgccaaa gtatcacaag ctgttatacc cgccagccgg gtaagatacc tgagtgagat
     3661 cagtgagaac aaccgaaatt atgatcagtt ggtaaaagac caggcggcca ttgccagtaa
     3721 gctatatcaa ctgcatggag caaaggaaac attaagagaa aaagcagaca agcccctgct
     3781 ggatgtggta gaacagcaga tcagtttcta tacggaacag ctgactcctg ttgcccgtaa
     3841 actgattacc aactggccca ataaggtgac tgagtatcag aaggattttt atgagtatac
     3901 ggtaagggat aaagtgatta aacaaccgat gaccaccaaa agtcttagcg gtacaaggat
     3961 ccctaaagtt gttttaccaa aatataatga ctggggtgat attttgaagt ggcaggcgca
     4021 ggaaaatgtg gccggtcatt tccctttcac agctggtgta tttcccctga aaagagaagg
     4081 ggaagaccca acaaggatgt ttgccggaga aggcggtccg gagagaacga acaaacgatt
     4141 tcattatgta agtgtggacc agccggccaa acgattgtca actgcgtttg acagtgtgac
     4201 actttatggt gaagaccctg attatcgtcc tgacatatat ggtaagatag gcaacagcgg
     4261 tgtaagtatt gccacggtgg atgatgccaa aaaattatac agtggttttg atctttgtga
     4321 tcccaaaacc tcagtttcta tgaccattaa tggtccggcg ccgatgctgc tggccttttt
     4381 catgaatgcg gcaattgatc agcaatgtga gaaatggata atggaaaatg gagaagggaa
     4441 gatggtagcg gatgcgctga agaaaaaata tcctgacgga aaaattcctg tttataataa
     4501 tccatcattc cccggccagc ttccggaggg gaataacgga cttggtttaa agctgcttgg
     4561 ccttagcggt gatgaggttt tgcctgctgc aatttatgaa caatgcaaac agtcagcgct
     4621 gcagcaggta aggggaacag ttcaggctga tata
//
LOCUS       DAORLS010000241         4651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10113_length_4651_cov_1.664273, whole genome shotgun sequence.
ACCESSION   DAORLS010000241 DAORLS010000000
VERSION     DAORLS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10113_length_4651_cov_1.664273"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    239..331
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<332..>818)
                     /locus_tag="PK977_15225"
     CDS             complement(<332..>818)
                     /locus_tag="PK977_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRF19526.1"
                     /translation="GAFAEMLKLPMGEYDMAHYAYEIERIDLKLAGGKQDQYAATFGG
                     VNFMEFYGDDKVIVNPLRVKQQHLFELENNLLLYYTSTSRESAEIIKKQSKNVTEKKE
                     KSIEAMHHLKDQARMMKEALLKGRLNEIGEILDYGFQQKRQMAEGISNTLMEDIYETA
                     K"
     assembly_gap    819..828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<829..1191)
                     /locus_tag="PK977_15230"
     CDS             complement(<829..1191)
                     /locus_tag="PK977_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRF19527.1"
                     /translation="MIYRSKAPLRIGLAGGGTDVSPYSDLYGGAILNATLSLYAKATI
                     EPIAENKIILEAIDRNEEQVFDWDTSLPVNGTLDLLKGVYNRVQQDYGVKQQGFRLST
                     FVDAPAGSGLGTSSTLVVA"
     gene            complement(1219..>1894)
                     /locus_tag="PK977_15235"
     CDS             complement(1219..>1894)
                     /locus_tag="PK977_15235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19528.1"
                     /translation="SEKVMNDLRLFEKNKPAQLISHPLYDNFGDIISKTEARKHLGLP
                     ANEKIILFFGFIRKYKGLDLLFEAMSHLNNLKLLVAGEFYEDEKPYKELIEKLGIQQQ
                     LILRTDFIPDSEVKYYLCAADAVIQPYRNATQSGVTPLAYHFEKPMVVTNVGGLPSLV
                     PHEKVGLVVEPDPAAIAEGIMRFYQLGEDYFIPHLRNEKQKYSWSSLVKAIQQLAEAV
                     ETGQRK"
     assembly_gap    1895..1988
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1989..2359)
                     /locus_tag="PK977_15240"
     CDS             complement(<1989..2359)
                     /locus_tag="PK977_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HRF19529.1"
                     /translation="MAKVVIIGPAHPLRGGLATFNQRLAKQFNDDGHECSIYSFSLQY
                     PSFLFPGKTQYTAEPAPEHLVINTVINSVNPFNWIKIGNRLKKEKPDLIIVRFWLPFM
                     GPSLGTILRQIRKNQHTKIIC"
     gene            complement(2359..3300)
                     /locus_tag="PK977_15245"
     CDS             complement(2359..3300)
                     /locus_tag="PK977_15245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF19530.1"
                     /translation="MDLSIVIPLLNEEESLPELSAWIEKVMTEHNYSYEVIFVDDGSN
                     DNSWEVIEKLRSQNASVQGIKFQRNYGKSAALNEAFRVASGDVVITMDADLQDSPDEI
                     PELRRMIMEDGYDMVSGWKKKRYDNTLTKNIPSKFFNAVTRSVSGIKLHDFNCGLKAY
                     KNRTVKGIEVYGEMHRYIPVLAKWAGFKKIGEKVVEHRARKFGVSKFGWNRFVNGFLD
                     LLSITFVGKFSKRPMHFFGLWGTLFFLLGLGIAVYLIVSKFMDSSFALTNKPGFYLAL
                     TSLIIGMQLFLAGFIGELISRNSPARNSYLVEKKTGL"
     gene            complement(3305..3787)
                     /locus_tag="PK977_15250"
     CDS             complement(3305..3787)
                     /locus_tag="PK977_15250"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRF19531.1"
                     /translation="MTLSPALKGAITAAAMIAIALITYYSGMPADSSFQYLVYVAYAA
                     GIIWTILAYKNSEKYTGRFWDNFNQGFRCFIIVTLIMVLFTAVFSKMHPEFAEESAKL
                     YKEDLVKSNSKTPAEIDEVVKKYKNGYTMMLVYSSIFGYLIIGAIITAVTAALTTRRS
                     "
     gene            complement(3797..>4651)
                     /locus_tag="PK977_15255"
     CDS             complement(3797..>4651)
                     /locus_tag="PK977_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19532.1"
                     /translation="EMYDMKNSGAIAFSDGLNCVQSSGLLVKALQYVKAFNGTIIQLP
                     DDKSINPHGLMNEGIISTQLGLPGKPAMAEELIVARDIKLARYADSKLHFTGISTKKS
                     LEYIKRGKESGAAVSCSVTPYHLFFCDEDLTGYNTNLKVNPPLRTKEEKESLKQAILN
                     GTVDCLASHHLPHEFDSKVIEFEYAKYGMTGLETAYAVLNTAIPGISTERCVELLAIN
                     PRKLFGMDAATINTGSKASLTLFNPARQWAVDEKDFKSKSSNSPFIGKQLTGKAIGII
                     TKGKIILA"
BASE COUNT         1147 a          985 c          945 g         1377 t
ORIGIN      
        1 ctgtttccat ttctaaaagc agttgtaatc aagtcaacca catttccaat ggtatcaatt
       61 aaacgggcat ctttcaagat ttgctccttt gttgttatag atgccttgat gatttgcctg
      121 attttttcca taataaccaa ttaacgaatt aaccagtaac taaactgtcc atgtttttaa
      181 tccatgctcc acaaactggt agttgcgaac ctgcccgcca aagttctcca aactttttnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttttggct gtttcataaa tatcttccat
      361 cagggtatta ctgatgccct cggccatttg tctcttttgc tgaaagccgt agtccaggat
      421 ttcaccgatc tcgttcagcc gtcctttcag caaggcttct ttcatcatcc gggcctggtc
      481 tttcaggtgg tgcattgctt ctattgactt ctcttttttc tccgttacat ttttgctttg
      541 tttcttgatg atctctgcag attcacggct ggtggaggta taatataaaa gaaggttatt
      601 ttccagttca aacaaatgtt gctgttttac ccgcagggga tttacaatca ctttatcatc
      661 tccataaaat tccatgaagt tgacaccccc aaaagtggcc gcatattgat cctgcttacc
      721 tccagccagt ttcaggtcta tgcgttcaat ttcgtacgca taatgggcca tgtcatattc
      781 tcccatcggc agtttcagca tttcggcaaa tgcaccgann nnnnnnnngg cgacgaccaa
      841 tgtagaagaa gtacctaaac cggagccggc tggtgcatct acaaaggttg acaggcgaaa
      901 gccctgttgt ttaactccat aatcttgctg aaccctgtta taaactcctt tcagcaggtc
      961 aagtgtaccg ttaacaggca aggaggtatc ccaatcaaaa acctgttctt cgtttctgtc
     1021 gatggcttca aggattattt tattttctgc aatgggttca atcgtggctt tggcataaag
     1081 ggaaagcgta gcattcagga tagcaccgcc gtaaaggtcg ctgtaaggac taacatctgt
     1141 gcctccgccg gcgaggccta tacgtagagg ggctttgctt ctgtagatca ttgaccgctg
     1201 attgcactga tttttttact atttgcgctg accagtttcc actgcttctg caagttgttg
     1261 gattgccttc accaggcttg accagctata cttctgtttt tcattgcgaa gatgcggaat
     1321 aaaataatct tctcctaatt gatagaaacg cataatgcct tctgcgatag ctgcaggatc
     1381 aggttcaaca accagtccca ctttctcatg cggtactaat gaaggtagcc ctccgacatt
     1441 ggttactacc atcggttttt caaaatgata agcgagggga gtaacaccgc tttgtgtggc
     1501 attgcggtaa ggttgtatca ccgcatcggc agcacagaga tagtatttta cttcactgtc
     1561 gggaatgaaa tcggttcgca ggatgagctg ctgttgaata cccagttttt ctattagttc
     1621 tttgtatggc ttttcgtctt cgtagaattc accggcgact aaaagtttga ggttgtttag
     1681 gtgcgacatt gcttcaaaaa gcaaatcaag tcctttgtat ttgcgtataa agccgaagaa
     1741 tagaataatc ttttcatttg caggcaggcc caggtgtttc cgggcttctg tttttgaaat
     1801 gatatccccg aaattatcat acaaaggatg ggagatcaat tgtgccggct tgttcttttc
     1861 gaacaggcgc aggtcattca tcaccttttc actcnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnat gcagatgatc tttgtgtgct gattctttct tatctgccgg agtattgttc
     2041 ccagtgaagg acccatgaaa ggcaaccaga accggacgat aattaaatcc ggcttttctt
     2101 ttttcagccg gttgccaatc tttatccagt taaacgggtt gacagaattg attactgtat
     2161 taatgacaag atgctccgga gccggttcag ccgtgtattg tgttttgccg ggaaaaagaa
     2221 aggaggggta ttgcagtgag aaagaataaa tagaacattc atgaccgtcg tcattgaact
     2281 gtttggccag ccgctggttg aaagtagcca ggcctccccg caacggatgg gcaggtccta
     2341 tgatgacgac ctttgccatt acagtcctgt tttcttttct acgagataag aatttcttgc
     2401 cggcgaatta cgggatatca gctcaccaat aaaaccggcg aggaacaatt gcataccaat
     2461 aataagtgat gttaaggcca ggtaaaaacc gggtttgttg gtcagggcaa aactgctgtc
     2521 catgaatttg gaaacgatca ggtaaacggc aatgcctaaa cccagcagga aaaacaaagt
     2581 accccagaga ccaaagaagt gcatgggtct cttagaaaac ttgcctacga atgtgataga
     2641 aagaagatcc aaaaagccat ttacaaaacg gttccagcca aatttggata ctccaaattt
     2701 tcttgccctg tgttctacca ccttctcacc aattttttta aaccctgccc atttggccaa
     2761 taccggaatg tagcggtgca tttcgccgta tacttcaatg ccctttaccg tacggttttt
     2821 ataagctttc agcccgcaat taaaatcatg cagtttgatg ccggacacac ttcttgtaac
     2881 tgcattaaaa aatttggaag gaatattttt ggtcagtgtg ttatcgtagc gttttttctt
     2941 ccagccactc accatatcat aaccatcttc catgatcatg cggcggagtt ccggtatctc
     3001 atccgggctg tcctgcagat cagcatccat cgtaatcacc acatcaccgg aggcgacacg
     3061 gaaagcttcg tttaaagctg ccgatttacc atagttgcgc tggaatttga tgccttggac
     3121 gctggcgttt tgggaacgca gtttctcaat tacttcccag gaattatcat tactgccatc
     3181 atccacaaaa atgacttcgt aactgtagtt atgttcagtc attacctttt caatccatgc
     3241 ggacagctca ggcaatgatt cttcttcatt taataatggt atgacgatgg acaggtccat
     3301 aaaattagct tctgcgtgta gttaaagctg ctgtaacagc cgttataata gcaccaatta
     3361 tcaaatatcc gaaaattgaa ctgtaaacca gcatcatggt gtagccgttt ttgtatttct
     3421 tcaccacctc atctatttct gccggtgttt tggaattgga tttaacgagg tcctctttat
     3481 aaagttttgc tgattcctcg gcgaattcag gatgcatttt gctgaaaaca gcggtgaaca
     3541 ataccatgat cagtgtgaca ataataaaac agcggaagcc ctggttaaaa ttgtcccaga
     3601 acctgccggt atatttttca gaatttttgt aagcaaggat tgtccagata atacccgctg
     3661 catatgccac atacaccagg tattgaaaac tagaatcggc cggcataccc gaataataag
     3721 tgatcagtgc aatagcaatc atagctgccg cagtaatggc tcctttcaat gctggtgata
     3781 gtgtcatagt ctgtaattag gcaaggatga ttttcccctt agtaatgatg ccaatggctt
     3841 tgccggtcag ttgttttccg ataaatggag agttggatga ttttgatttg aaatcctttt
     3901 catctactgc ccattgcctg gcaggattga aaagagtgag tgatgcctta ctgccggtat
     3961 tgatggttgc ggcatccata ccgaaaagtt ttcttggatt tatcgccagc agttctacac
     4021 atctttctgt gctgatgccg ggaatagcgg tattcaatac tgcatacgcc gtttccaatc
     4081 cagtcatacc gtatttggca tattcaaatt cgatgacctt gctgtcaaat tcatgtggca
     4141 ggtgatgtga agccaggcaa tcaaccgttc cgttaagtat ggcttgtttc agcgattctt
     4201 tttcttcttt tgttcgtaaa ggagggttta ctttcaggtt ggtattatat ccggtaaggt
     4261 cttcatcaca aaagaagaga tgataaggtg tcaccgaaca gctaaccgct gcgccgcttt
     4321 cttttcctct tttaatgtat tccagagatt tttttgtgct gatacccgtg aaatgcagtt
     4381 tggagtctgc atagcgggcc agtttaatat cccttgccac gatcagttct tctgccattg
     4441 ccggtttgcc gggcaggccc agctgtgtgg atataatacc ttcattcatc aaaccatgcg
     4501 ggttgatact tttatcatcg ggcagttgta tgatggtgcc gttaaatgct tttacatatt
     4561 gcaaagcttt taccagcagc ccggacgatt gtacacagtt cagtccgtca ctaaaagcaa
     4621 tggcaccgct gtttttcatg tcatacattt c
//
LOCUS       DAORLS010000242         4621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10199_length_4621_cov_1.579063, whole genome shotgun sequence.
ACCESSION   DAORLS010000242 DAORLS010000000
VERSION     DAORLS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10199_length_4621_cov_1.579063"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>336)
                     /locus_tag="PK977_15260"
     CDS             complement(<1..>336)
                     /locus_tag="PK977_15260"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF19533.1"
                     /translation="NSNPADGTTTQWNWTMGDGNVRTTPSFTYTYGSAGTYDVSLYIT
                     NSFGCRSTTFTQQVTVNPYPVVDAGPDLFILEDGSDTLQPIVTAINPSFLWTPNQYFL
                     SSNTIERPI"
     assembly_gap    337..453
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    767..839
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<840..>1146)
                     /locus_tag="PK977_15265"
     CDS             complement(<840..>1146)
                     /locus_tag="PK977_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19534.1"
                     /translation="GIYSGPGVTTGGIFTPSSVAPGGTYTIQYLHTSTAAGCRDSATQ
                     TITVIDTASARFVVSNLACERSPVSFNSSSSTIPPTAGTITGWQWNFGDPASGAANTS
                     "
     assembly_gap    1147..1251
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1252..>2101)
                     /locus_tag="PK977_15270"
     CDS             complement(<1252..>2101)
                     /locus_tag="PK977_15270"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF19535.1"
                     /translation="TSGFTVVTPSCETRITSFTDASVPNAGNITSWQWNFGDPASGPL
                     NTSTLQNPTHIFSTAGTYNVSLVVTTDKGCVSTTPPQQVVINARPLAGFILPEVCLSD
                     TYAQFTDTSSVDAPDFIQAWNWNFGDPGSGPLNTSTLQNPQHSYTATGSYNVQLIATS
                     NNGCRDTVTQVLFINGSFPLADFTIQNPSTLCANDSVAIVEASTVFPGTITKVEIYWD
                     NVNQPAVFDTDNSPFTGKVYKHLYPNFQAPLTRTFTIRYRAYSGGVCVNDKLQDITVN
                     AAPLVQF"
     assembly_gap    2102..2112
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2113..>2549)
                     /locus_tag="PK977_15275"
     CDS             complement(<2113..>2549)
                     /locus_tag="PK977_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19536.1"
                     /translation="TVTTTSGNSVTVAAPTNTPGTYTYSLVSVSDGSTTSCSQLQSGS
                     AIVTVNPLPTASISGNTEVCLNAPSPNITFTGATGTAPYTFTYTINGGPNQTVSTTTG
                     NSVTVSVPTSVAGTFTYNLISVLDGSSTLCSQAQTGSATVIVN"
     assembly_gap    2550..2577
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2578..3420)
                     /locus_tag="PK977_15280"
     CDS             complement(<2578..3420)
                     /locus_tag="PK977_15280"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF19537.1"
                     /translation="MGIADVTLNAPVPTSTIVVAGKTLYVYQLPTPYTITAAGTYPIR
                     VVANNPTPDGCSGVQEIDFDVQVFDPPVADFTFNNVCFPDPVQFNDNSNTGGRPIASR
                     YWNFGDANTSIANNPTHVYAAPGAYTVKYAVITDVGCLSDTASHIVTVSPLPTATVSG
                     ATDVCLNAPSPNVTFTGAVGTAPYTFTYNINGGPNQTVTTTSGNSVTVSVPTNTVGTF
                     TYNLVSVQDASPNLCSQAQTGSVVINVNPLPTATIDGTITVCENAPSPNITFTAGGGA
                     TAPYT"
     assembly_gap    3687..3741
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3742..>4621)
                     /locus_tag="PK977_15285"
     CDS             complement(<3742..>4621)
                     /locus_tag="PK977_15285"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HRF19538.1"
                     /translation="IHITSDKPIVAYAHIYNQSVSGATILFPTPTLGKEYYSINYQNT
                     SNTNNANCWFYVIAADTGTTTVEIVPSGATTNGWAAGSTNTITLTQGQVFNVMGQLTT
                     TTNPFQGVDLTGSLIRSVAGPNGSCKRIAVFSGSGRISITCNAGSSSSDNYMVQAFPK
                     SAWGKKFLTASTAGNQQNNIYRVCVSDPTTVVTLNGAPIGLPLQNGFYYQIASSNTPK
                     LIEADKPITVAQYLTSQGACGNGAQPGDPEVIYLSPVEQNISKVLWNATPNFQILQHY
                     FNVIIPNTRTAISSFTL"
BASE COUNT         1160 a          855 c          982 g         1235 t
ORIGIN      
        1 caatgggtct ttcaatcgta ttactgctca ggaaatactg gttaggtgtc caaaggaagc
       61 ttggatttat ggctgttacg attggctgta gtgtatcact gccatcctcc aatatgaata
      121 aatcaggacc ggcatccaca acagggtaag gattcactgt tacctgctga gtaaatgtgg
      181 tactccgaca accaaagctg ttcgtaatat acaagcttac atcataagtg ccggcactac
      241 catatgtata ggtgaatgat ggcgttgttc ttacattgcc atcccccatc gtccagttcc
      301 attgtgtggt ggttccatct gccggattac tgttgtnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggtgtc tcttgcacaa ccaactccgc
      481 tggtaacctg taaattaacg ttgtatggac ctactgcatt atatatatgt gatggtgctg
      541 aaccggtaga ggtattcaat ggaccgctgc ccggatctcc aaaattccat aaatagctaa
      601 atgatgcctg tgaaccatct ggtatggtac tcaggttggt gaattgcaca tttgccgaag
      661 gcaaacaact acttgccggg aatgagaaat taggtcttgg aataggattc accacaaccg
      721 gcatattaaa tgcagtactt cggcaaccat tgcttgtagt tacaacnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
      841 gaggtattgg ctgcaccgct ggcagggtca ccaaagttcc attgccagcc ggtaattgtg
      901 ccggcagtcg gcggaatggt tgaactgcta ctgttaaaac taaccggact cctttcacag
      961 gcaagatttg aaacaacaaa tcttgctgaa gccgtatcaa taaccgttat tgtctgtgtg
     1021 gcagaatctc tgcaaccagc cgctgtggaa gtatgcaggt attgaatagt ataggtgccg
     1081 cctggtgcaa cagaagacgg tgtaaaaatt ccgcccgttg taacgccggg acctgaataa
     1141 ataccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaactgaac
     1261 caacggagcc gcattgacag ttatatcctg taatttatca tttacacaaa ccccaccgga
     1321 ataggccctg taacgtatgg taaaggttcg ggtgagcggt gcctggaaat taggatataa
     1381 atgtttgtaa acttttcctg taaacggtga attatctgtg tcgaatactg caggctggtt
     1441 tacgttatcc cagtaaattt caactttagt tattgtgccc gggaaaacgg tagaagcttc
     1501 tactattgct acagaatcat tggcacaaag agtagatgga ttctgtatag taaaatcagc
     1561 taatgggaaa ctaccattaa tgaaaagaac ctgtgttacg gtatccctgc acccgttatt
     1621 gctggtagca attaattgta cattatatga tcctgttgca gtgtagctgt gttgaggatt
     1681 ctgtaatgtg gatgtattaa gaggtccgct tcccggatct ccaaaattcc aattccatgc
     1741 ctggatgaaa tccggcgcat ctacactgct ggtatctgta aactgggcat aggtatcact
     1801 taaacaaact tcaggaagta tgaaccctgc taatggccgg gcattgatca ctacttgttg
     1861 tggcggtgtg gtactaacac aacctttgtc tgttgtaact acaagactta cgttatatgt
     1921 tccagccgtg gagaaaatat gggttggatt ttgtaaggtc gaagtattta aaggaccgct
     1981 tgccggatct ccaaaattcc actgccagga agtaatattt ccggcattgg gcacggaagc
     2041 atccgtaaaa cttgttatcc ttgtttcgca ggatggggta accacagtaa aaccactggt
     2101 tnnnnnnnnn nnatttacta ttaccgttgc cgatcctgtt tgtgcctggc tgcacaatgt
     2161 agaactacca tcgagaacgc tgatgagatt gtaggtaaat gtgccggcaa ccgaggtagg
     2221 aacgctaacc gttaccgaat ttccggtagt tgttgaaact gtttgattgg ggcctccatt
     2281 aatagtataa gtaaaagtat aaggtgcggt accagttgcc cctgtaaaag taatattggg
     2341 agaaggagca tttaaacaaa cttcggtatt gcccgagata ctggctgtag gcaatggatt
     2401 aaccgtaact atggctgagc cgctttgcaa ctggctgcag gaggtagtac ttccatctga
     2461 cacacttacc aggctgtatg tatatgtacc cggtgtattt gtcggagcag ctactgttac
     2521 tgaattacca ctggtggttg tcaccgtttn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt
     2581 atagggtgcg gtagcccctc ccccggcggt aaaagtaata ttgggggacg gtgcattttc
     2641 gcatacagta atagttccat caatggtcgc agttggcaaa gggttcacat tgattaccac
     2701 agagcctgtt tgtgcctgtg aacataaatt agggctggca tcctgtacac ttaccaggtt
     2761 gtaggtaaat gtccctactg tattggttgg cactgaaacc gtaacactgt ttccacttgt
     2821 tgttgttact gtttggttgg ggccaccgtt aatattgtat gtaaaagtat aaggggcagt
     2881 gcccacagca ccagtaaatg tcacattagg tgaaggtgca ttcaagcata catctgttgc
     2941 gccgctaacc gttgcagttg gcaaaggtga tacggttaca atatgtgaag cggtatcaga
     3001 taaacaaccc acatctgtaa ttacagcgta tttgactgta tatgcaccgg gtgctgcata
     3061 aacatgggta gggttattag caatactggt gttggcatcg ccaaaattcc aataacggct
     3121 tgcaataggc cgtccacccg tatttgaatt atcattgaat tgaacaggat cagggaaaca
     3181 aacattatta aaagtaaaat ctgccaccgg gggatcaaaa acctgtacat caaaatcaat
     3241 ttcttgcaca ccactgcaac catctggtgt tgggttattt gccaccaccc tgatcgggta
     3301 agttcctgct gctgtaatgg tatatggagt tggcaactgg tacacatata gtgttttgcc
     3361 ggccactact atggttgatg tgggtactgg tgcattgagt gttacatcgg cgattcccat
     3421 ggcatttagc gcagctccaa aaatccattg gatctgtgtt ggctggtatg gaaaggtcat
     3481 tgaaaaatag aaaggcgtac tgcgacaggt agcagggaaa tttaccgttg catattgatt
     3541 ttgaagggat atatattgaa atatatcttt aatgtttgtc ccggcattat acccatatga
     3601 ttctgcattt ccatagccat aggctatagc gttaaatccg gaatctgatt gtattatatg
     3661 ttgacccgct gcaactgact gacgtannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nagggtaaag gagctaatgg ctgtccgggt gttgggaatt
     3781 attacattga aatagtgttg aagtatctga aagttaggag tcgcattcca caatacttta
     3841 ctaatatttt gctctaccgg gctaaggtaa atcacttctg gatctcctgg ctgagcacca
     3901 tttccgcatg caccttgtga ggtaaggtat tgagccacgg taataggttt atcagcttct
     3961 attagttttg gcgtattaga cgaagctatt tgataataaa atccattttg cagcggtagc
     4021 ccaattggtg caccatttaa tgttactact gtagttggat cagaaacaca taccctataa
     4081 atattgtttt gttgattacc agcagtagat gcggttaaaa atttcttacc ccaggccgat
     4141 ttaggaaatg cctgaaccat ataattatct gaagaagatg agccagcatt gcatgtaata
     4201 ctaattcgtc cgcttcctga aaaaactgca attcttttac acgaaccatt agggcctgca
     4261 acacttctga taagtgatcc tgtcaaatca accccctgaa aaggattggt agttgtagtg
     4321 agctgcccca ttacattgaa aacttgtccc tgtgttagag taatggtatt agtgcttcct
     4381 gcagcccagc catttgttgt tgcacctgat ggtacaattt cgactgttgt agtcccagtg
     4441 tcagctgcta ttacataaaa ccagcaattt gcattgttgg tgttggatgt attctgataa
     4501 ttaatggaat aatactcttt acctaaagtt ggggtcggga aaagaattgt tgcgcctgat
     4561 acactctggt tataaatatg cgcatatgct actatcggct tatcactggt aatatgaatc
     4621 c
//
LOCUS       DAORLS010000243         4596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10262_length_4596_cov_1.489320, whole genome shotgun sequence.
ACCESSION   DAORLS010000243 DAORLS010000000
VERSION     DAORLS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10262_length_4596_cov_1.489320"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>386
                     /locus_tag="PK977_15290"
     CDS             <1..>386
                     /locus_tag="PK977_15290"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRF19539.1"
                     /translation="ILGSLRCDCGDQLQSAMQMVEKEGKGAILYMNQEGRGIGLLNKL
                     KAYRLQEQGMDTVEANLHLGFKMDQRDYGIGAQMLRQLGITRLRLMTNNPKKRVGLIG
                     YGLEIVENVPIEACPNPYNEKYLHTKR"
     assembly_gap    387..430
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(432..>779)
                     /locus_tag="PK977_15295"
     CDS             complement(432..>779)
                     /locus_tag="PK977_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19540.1"
                     /translation="NRFIISTGIGFDAPLQQSNIQQSIQLLPDVTLEWLINPSGSIRA
                     SFFYRENADFLNISSSGPGRARRYGASLSYRKDFDNLGDIFKSKKKKQQQAAAQKPEE
                     TEEVKKEDAPNEN"
     assembly_gap    780..832
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<833..>1799)
                     /locus_tag="PK977_15300"
     CDS             complement(<833..>1799)
                     /locus_tag="PK977_15300"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRF19541.1"
                     /translation="NVKGTGSLSLLGPQANMYMKIDAIASSTDSSSITLPNSSSRESG
                     IADFLVERKYGREMTDSDFVSNATNIIYDVDVTANPMVNVKVILDELTGDEIKGRGTG
                     TLNIRSGTSEPLSLRGRFDIAEGSYVFTFQSFFKKPFELKKGVDNYIEWNGDPYDANI
                     NFTAQYTAEKVSFAPLAKLKQFSSGAANARDNVFVVAKLTDKLFKPSIQFSLDFPGTS
                     VAVTDPELALIIQQLQKNINEINRQVTYLIVFNSFAPSELSADTEGGGFNLNTISGIL
                     LGVVSDQLNKILGNLLKNDKFNISLNTSLYNRNIIDPNNKTALNLG"
     assembly_gap    1800..1922
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1923..>3515)
                     /locus_tag="PK977_15305"
     CDS             complement(<1923..>3515)
                     /locus_tag="PK977_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19542.1"
                     /translation="TIDFNSKTPRFDLIAKVDKAELKSLRLTKDSLTFKGNLTLNFTG
                     KNIDDFLGTARITDAEITKDGTRLPFDSLVLSSVIEDHEKKLTVESNEFKADINGNFS
                     LTDLPNSFTYLLNKYYPAYIKAPKKMPLDQDIRFDINTYYIDEYLQLFIPELSGLNNS
                     TISGNLNLAQNELNLNVKAYQFKYKQYNFEDVTILAQGSGNNLSLTGGITKIQVNDSL
                     SIPNVSFNIDAHNDSSIVKIKSGVNRTVDTANLNALVLTYNDGVKIEFDPSTFTINGK
                     TWSIDESGELVLRKNTPAGGQLVLSEGEQKIVLRSEKSPKGNWNDVKVQLTKINLGDF
                     APYFMPKNRLEGLLSGNIIVEDPVNNLSIRSDDLQTQFLRLDNDSLGEVKVTAAYEKA
                     TKLLKVKGNTLNQENYLGFDANIYIGDPEKAKNNLIALKAKDFQLSVLERFLGTLFTD
                     IQGFLTGDISIAGEFDHLAITGKGRLKNAGLKVIFTQCFYKIQDTDIQLTPEEINLDG
                     IVLTDTVTKNPIYITGGIEHESF"
     assembly_gap    3516..3551
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3552..>4596)
                     /locus_tag="PK977_15310"
     CDS             complement(<3552..>4596)
                     /locus_tag="PK977_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19543.1"
                     /translation="VMKVNELRIINGTFKTDKQTDREAFTYFDGKHILFKEINATFKN
                     ASFLADTITTGMDLTAKERSGLEVKKFLADVKMTPQGMAFSNMDLQTNRSTLRNFYSM
                     SYNHMDDLGDYIHKVRMAAIFTDSYIDSDDIAFFAPGMKTWKKKITLKGKVRGTVDDL
                     VGRDMLVEAGNSTVLNGDITLTGLPDINQTFIDFKANDFRTTYNDAVTFIPAVKKVTS
                     PDLRKIQYLNFRGSFTGFIRDFVTYGTITTNLGTITSDVNMKLPKGQDPVYSGAIATQ
                     NFRLGELLGDKNLGAISMNGTVKGRGFNEKSRNTLIDGNVQFIEYNGYRYENITMNGR
                     LDKKMFEGVASLKD"
BASE COUNT         1122 a          885 c          939 g         1394 t
ORIGIN      
        1 atactgggtt ctcttcggtg cgactgtggc gatcagttgc aatcagccat gcaaatggtg
       61 gaaaaagaag ggaagggggc tatattatat atgaaccagg aaggaagagg tatcgggctt
      121 ttgaacaaac tcaaagcata ccgactccag gagcagggga tggataccgt ggaggccaac
      181 ctgcatcttg gttttaaaat ggatcagcgg gactatggaa tcggtgccca gatgctccgc
      241 cagctcggca tcacccggct tcgtttaatg accaataacc cgaaaaaaag agtaggactg
      301 attggttatg gattagagat tgttgaaaat gttcctattg aagcctgccc gaatccttat
      361 aatgaaaaat acctgcatac aaagcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn attagttttc gtttggtgca tcttcttttt ttacttcttc cgtttcttca
      481 ggcttttgag cagcagcctg ttgctgtttc ttttttttgc ttttgaagat atcgcccagg
      541 ttgtcaaaat ccttgcgata acttaaactg gcgccatacc ttctggccct gccggggcca
      601 cttgatgata tgttcaggaa gtctgcattt tctctgtaaa aaaatgaagc ccggatactt
      661 ccgctggggt ttatcagcca ctccagagtt acatccggca ataactggat actttgttga
      721 atattcgatt gctgtaacgg cgcatcaaaa ccgataccag tggatatgat aaacctgttn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcccaggt
      841 taagtgcagt tttattattg ggatcaatta tgtttctgtt atatagagaa gtattcaggc
      901 tgatattgaa tttatcattc ttgagcaggt tgcccagtat tttattcaac tggtcactga
      961 ccactccgag caaaatacct gaaatggtat tcaaattaaa gccacccccc tcagtatctg
     1021 cagaaagctc actgggagca aaactattga atacaataag gtaagtaacc tggcggttga
     1081 tctcgttgat gtttttttgc aattgttgaa tgataagagc aagttcagga tccgtaacgg
     1141 ctacactggt gcccgggaaa tcaagcgaga actggatgga aggtttgaaa agtttgtctg
     1201 tgagtttggc aacaacaaat acattgtccc ttgcgttagc tgcaccggag ctaaactgtt
     1261 tcaatttcgc cagcggtgca aaactgactt tttccgccgt gtattgcgca gtgaagttga
     1321 tattggcatc ataaggatcg ccgttccatt caatgtaatt atctactcct ttcttcagtt
     1381 caaatggttt tttgaagaaa gactggaaag taaagacata gctaccttca gcgatatcaa
     1441 aacgtccacg cagcgacagt ggctccgatg taccggagcg gatattcaat gtgccggtgc
     1501 ccctgccctt gatttcatca ccggtgagtt catccagtat cacttttaca tttaccatcg
     1561 ggttggcagt tacatccaca tcataaataa tattggtagc attggagaca aaatcactgt
     1621 ccgtcatttc tctaccgtat tttctttcta ccaggaaatc agcaatgccg gactccctgc
     1681 tcgacgagtt gggcaatgtg atgctgctgc tgtctgtgga agatgcaatg gcgtcgatct
     1741 tcatatacat gttggcctga ggcccaagca gcgacaaaga accagtacct tttacattgn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nngaacgatt catgttcgat accgccggta atatagattg ggtttttggt cactgtatct
     1981 gtaagcacta taccatccag gttgatctct tccggggtta actgaatatc tgtgtcctgg
     2041 attttataaa agcattgggt gaatatcact tttagtcctg cattcttcag ccttcctttg
     2101 ccggtaatgg ccaggtggtc gaattctccg gcaatactga tatcgcctgt cagaaagcct
     2161 tgtatatcgg taaacaatgt accaaggaaa cgttcaagca cacttaactg aaagtctttt
     2221 gcttttaagg caatcaggtt attctttgct ttttcaggat cgccgatata gatattggca
     2281 tcaaaaccaa ggtagttttc ctgattcagg gtattgcctt ttacttttag cagttttgtt
     2341 gctttttcat aagcagctgt aacttttact tcgcctaatg aatcattatc aaggcgtaga
     2401 aactgtgtct gcagatcatc agaacggatg gataaattgt tgaccggatc ttctactatt
     2461 atatttcctg ataacagccc ttcgagtctg ttctttggca taaagtaagg ggcaaagtct
     2521 ccgaggttga tttttgtgag ctgaactttt acatcattcc agttgccttt aggagatttt
     2581 tcagaacgta acacaatctt ttgctctcct tcgctgagca ccaattgacc accggccgga
     2641 gtgttttttc gcaggacaag ttctccgctc tcatcgattg accaagtctt tccattgatc
     2701 gtaaatgtgg aagggtcaaa ttctattttg acaccatcat tgtatgttaa taccagtgca
     2761 ttcaggttgg ctgtatcaac tgttctgttt acacctgatt taatttttac tatagaagaa
     2821 tcgttgtgtg catcaatatt gaatgaaaca ttagggatgc tcaggctgtc gtttacctgg
     2881 atctttgtaa tgccccctgt aagtgagaga ttattaccgg aaccctgtgc caatattgtt
     2941 acgtcttcaa aattgtattg tttatactta aactggtaag cttttacatt caggtttagt
     3001 tcgttttgtg caaggttgag gttgccagag atggtactgt tgttaagccc ggataattct
     3061 ggtataaata attgcagata ctcatcaata tagtaagtat tgatatcaaa ccggatatcc
     3121 tggtcaagcg gcattttctt tggtgccttt atatatgcag ggtaatattt gttcaataaa
     3181 taagtgaacg aattgggcag atcagtcagg ctgaaattgc cattgatgtc tgctttaaat
     3241 tcgttggatt caacagttag ttttttttca tgatcctcaa tcactgaaga aagaactaac
     3301 gagtcaaatg gcaaacgggt gccatccttg gtgatctctg catctgtgat acgggctgtc
     3361 cctaaaaaat catcgatatt cttcccggta aagttgagtg taaggttgcc tttaaaagtt
     3421 aaactgtctt tcgttaaccg gagacttttt aattcggctt tatccacttt ggcaatgaga
     3481 tcgaaacggg gtgttttgct gttgaaatca attgtnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn ntgtctttca gcgaagcaac accttcaaac atttttttgt cgagccggcc
     3601 attcattgtg atgttttcat agcggtaacc attgtattca ataaattgta cgttgccatc
     3661 aatgagtgtg ttgcggcttt tctcgttaaa tcctcttcct tttactgttc cgttcatgga
     3721 gatggctcct aaatttttat cacccaggag ttcacccaat ctgaaatttt gtgttgcaat
     3781 tgcgccggaa tacacaggat cctggccttt tggcagtttc atgttcacat cgctggtaat
     3841 agtgcccagg ttggtagtga tggttccata ggtaacaaaa tcacggatga aaccggtaaa
     3901 actgccccta aaattcaggt attggatttt ccgcagatca gggcttgtaa cttttttaac
     3961 agccggtata aaggtgacag catcattgta agtggtcctg aaatcattcg ccttaaagtc
     4021 aataaaagtc tggttaatgt ccggcaaccc ggtgagggtt atgtctccgt tcaataccgt
     4081 gctgtttcct gcttctacta acatatccct gccaacaaga tcatcaaccg ttcctcttac
     4141 ttttcctttt aaagtaattt tcttcttcca tgttttcatg cccggggcga agaaggcaat
     4201 gtcatcactg tcaatgtaag aatcagtaaa tattgctgcc atcctcactt tatgaatata
     4261 atcgccgagg tcgtccatgt gattgtagga catggaatag aaattcctga gggtgctgcg
     4321 gttggtttgc aggtccatat tactgaaggc cattccctgc ggagtcattt ttacatcggc
     4381 gagaaatttt ttgacctcta atccgcttct ttctttggcg gtgagatcca tgccggtagt
     4441 aatggtatca gccaggaaac ttgcgttttt aaaggtagcg ttgatttctt tgaagagaat
     4501 atgtttgcca tcgaaataag taaaggcttc cctgtctgtt tgtttgtctg ttttgaatgt
     4561 gccgttgatg atcctgagct cattcacttt catcac
//
LOCUS       DAORLS010000244         4590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10282_length_4590_cov_2.111136, whole genome shotgun sequence.
ACCESSION   DAORLS010000244 DAORLS010000000
VERSION     DAORLS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10282_length_4590_cov_2.111136"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..72
                     /locus_tag="PK977_15315"
     tRNA            1..72
                     /locus_tag="PK977_15315"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Glu,seq:ttc)
     gene            690..1085
                     /locus_tag="PK977_15320"
     CDS             690..1085
                     /locus_tag="PK977_15320"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRF19544.1"
                     /translation="MNTLTTPIILSKEDHDLILFYLRSGKWRSPADSQSADKLEAELK
                     RAQIVNKKNLRKDIVQLNSLVKVRDLEANKLMELVIVTPDKANLKEMKISVLSPVGTA
                     LIGFGQGDYVKWKVPAGEKLFVIEEVIKD"
     gene            1199..1606
                     /locus_tag="PK977_15325"
     CDS             1199..1606
                     /locus_tag="PK977_15325"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HRF19545.1"
                     /translation="MKTINHPVILFDGICTFCNFWVNFAIKYDPKKRIKFAPFQSNAG
                     QLLLSQHHIAIESINSVILIENGKVFTQSSAALRICKYLSGLWKIFFGLLIIPKFLRD
                     FFYNIIAQHRYRWFGKKESCMVPTPEVRERFLD"
     gene            complement(1607..>1791)
                     /locus_tag="PK977_15330"
     CDS             complement(1607..>1791)
                     /locus_tag="PK977_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19546.1"
                     /translation="GNTILVISILAIIPAIVIMSKGHFMHNPKEFTLLLSNGYVWVAY
                     FAGAGGMAAILRNLDK"
     assembly_gap    1792..1829
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1830..2068)
                     /locus_tag="PK977_15335"
     CDS             complement(<1830..2068)
                     /locus_tag="PK977_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19547.1"
                     /translation="MTPQVKNIAWNTLAFILMTVAVCIVNYILQIIIIFMDVFSRRLE
                     ESVAYIIVLWLVTGIFGSVFTTGLAEHLVKKENFT"
     gene            complement(2093..2452)
                     /locus_tag="PK977_15340"
     CDS             complement(2093..2452)
                     /locus_tag="PK977_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19548.1"
                     /translation="MSKFPFALLTDEQVIDTWTLIYTPPGGGKYNGKLTVTNKRLLYD
                     AKYDVSAKGLLSEAMFVKWGSEGYLEISKSDISSVKAEKSFLAKKALVTLSDGSVHTF
                     NYGVLNIDKVIEAINQR"
     gene            complement(2472..3266)
                     /locus_tag="PK977_15345"
     CDS             complement(2472..3266)
                     /locus_tag="PK977_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRF19549.1"
                     /translation="MKLNLFQADAFTNKLFGGNPAAIVPLKKWLPDETMQQIAMENNL
                     AETAFFVPSKNKQADYDIRWFTPAIEINLCGHATLASAYLVFDILKAKKNKVIFSSKS
                     GLLTVTRGKKILEMDFPSWPPEKITEYPPELLASLGNPEITGVYQNREYLVELTNEDA
                     VKYCQPDFSLMKKLDRMVIITAPGKEVDFVSRFFAPTAGIDEDPVTGSAHSQLIPFWS
                     YKLGKQKMTARQLSKRGGELVVEQKGERVMMGGECVFYMKGKIQLP"
     gene            complement(3263..3892)
                     /locus_tag="PK977_15350"
     CDS             complement(3263..3892)
                     /locus_tag="PK977_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRF19550.1"
                     /translation="MPTQFLLIIDILGTFAFAVSGAFLAMEKKLDPFGVLVLSFVTAI
                     GGGTLRDILIGNLPVSWLQNETASIVIFSSAIATMFFGRYLKKLTITLFLFDALGLGL
                     FTIIGIELGVDKGFSTGICIALGTITACFGGVVRDVLLNNIPLLFRKEIYALACIAGG
                     LFYFLLREMRFDAVPSKILCILIIFTIRVMAVRFKWSLPQFYNKSITER"
     gene            complement(3967..4224)
                     /locus_tag="PK977_15355"
     CDS             complement(3967..4224)
                     /locus_tag="PK977_15355"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRF19551.1"
                     /translation="MPIVKVIEVIASSEISFDDAVKQAVKESSKTIRNIDSVWVKDMK
                     AHVKDGKISTYGVICHISFRVDNEEKKEKKKGKKNKDKNDE"
     gene            complement(4321..>4590)
                     /locus_tag="PK977_15360"
     CDS             complement(4321..>4590)
                     /locus_tag="PK977_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRF19552.1"
                     /translation="LPFDSNHSILESLKKTNRIVFVDEDVPGGAAAYMFNKVMEEQGG
                     YRYLDVAPRTITAKAHRPAYGSDGDYFSKPNAEDIEEVIRQMMAE"
BASE COUNT         1362 a          993 c          834 g         1363 t
ORIGIN      
        1 ggcccgttcg tctaagggct aggacaccac cctttcacgg tggtgatacg ggttcgaatc
       61 ccgtacgggc tacaaaaaaa ggctttgaat taattcaaag ccttttttaa tctgcttctg
      121 ttcaaaacag gttttagtat taccgtgata accaatacaa aaagcagtac tttcttcaca
      181 ttatattttg tatcttcata gctaatgagc aggtattcgt catacaatta tacttacctg
      241 tcaggtcagg caatcgcaga cctgacggat tgttatacct ggtcattccc cgaatttgtt
      301 cctttctgcg aacaactttt ttacttctcc acttttaaag cctttaaaag ctaagcccgg
      361 tctgatcaat tcgggaccgg cgacctttta tctgacagca acaagattcc tgtcagatgt
      421 actcttgctt ataccatgag taatgaattt tttcttgaac aattaattca acaacatgaa
      481 acttaaagaa gaatcagtgc atggcatgct gatactaatt ctgctattgc ttaattcagt
      541 gatcttagag accgcccttg taaaaaacca ccaatggtac aaggcccttt ggatcaccgt
      601 accaatactt ttgctctgcc tgctcgatca atggcggatt aacactgtct ttaaacgaaa
      661 acaataaaat aaaaaaattg taaactgaaa tgaatacttt aacaacccca ataatacttt
      721 ccaaagaaga ccatgacctt atcctgtttt acctgcgaag cggaaaatgg cgtagccccg
      781 cagatagtca gtcggctgac aaactggaag cagaattgaa aagagcccaa atagtaaaca
      841 aaaaaaatct tagaaaggac attgtccagc ttaatagcct ggtaaaagtt agggatcttg
      901 aggccaacaa actgatggaa cttgtaattg tgacaccgga taaagccaac ctgaaagaaa
      961 tgaaaatatc tgttctttct ccggttggca ccgcattgat tggctttgga cagggtgact
     1021 atgtaaaatg gaaggtgcct gccggggaaa agctcttcgt tattgaagaa gtaattaaag
     1081 attgacaggc aaatacaagc ataaagcagg cctctgacat aggcctgctt tttttaacta
     1141 gacaaatcaa ataatctgaa aaatacaaac cttcatagct ttaccagtta taagaagtat
     1201 gaaaacaatc aatcacccgg taatactctt tgatggcatc tgtacctttt gcaatttctg
     1261 ggtaaatttt gccattaagt atgatcctaa aaaaagaata aagttcgccc cttttcaaag
     1321 caatgcaggg caactgcttt taagccagca tcatattgct atagaatcca tcaattcagt
     1381 tatccttatt gaaaacggaa aggtttttac ccaatcttct gctgcattga ggatatgtaa
     1441 atacctctca gggttatgga agattttttt cggattactt atcatcccga aatttctccg
     1501 ggacttcttc tacaatatca ttgcccagca ccggtatcgc tggtttggta aaaaggaaag
     1561 ctgtatggtg ccaacaccgg aagtaagaga acgattttta gactgattat ttgtcaaggt
     1621 tcctgagaat ggctgccata ccacctgctc ccgcaaaata agccacccat acatacccat
     1681 tactcaagag caaagtgaac tctttgggat tatgcataaa atgtccctta ctcataataa
     1741 ctattgcagg aataattgcc aatattgaga taaccaatat ggtattgccg gnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aagtgaagtt ctcttttttt accaggtgtt
     1861 ctgctaaacc tgtggtaaat acagatccaa aaattcctgt caccagccac aaaacaatga
     1921 tataggcaac tgattcttca agcctcctgg aaaaaacatc cataaatatg attatgatct
     1981 gcagaatgta attgactata caaacagcta ccgtcattaa aatgaatgcc agtgtattcc
     2041 aggcaatatt ttttacttgt ggcgtcattg ctgattttat tgctatacca ttttaacgct
     2101 gatttattgc ttcaataacc ttgtcgatat tcaatacacc ataattgaac gtatgtacag
     2161 aaccgtctga cagggttaca agggcttttt tggccaaaaa acttttttcc gcttttaccg
     2221 aactaatatc tgacttacta atttcaagat agccttcact tccccatttt acgaacatag
     2281 cttctgacaa caaccctttg gccgatacat catactttgc atcgtacagg agccgtttat
     2341 tggtaacagt tagttttcca ttatacttac ccccgccggg aggagtataa atcagtgtcc
     2401 aggtatcgat tacctgttca tcagttagta gcgcaaatgg gaatttagac ataacagaag
     2461 gttataaata attaaggtaa ttgaattttt cctttcatgt aaaaaacgca ttcacccccc
     2521 atcatcactc tttctccttt ttgctcaaca accaattcac ctcctctttt tgacaactgc
     2581 cgggctgtca ttttctgttt gccaagtttg taactccaaa aaggaatgag ttgagagtgt
     2641 gccgaaccag ttaccgggtc ctcatcaata cccgctgttg gggcaaagaa cctggataca
     2701 aaatccacct cttttccagg tgctgttatg atcaccatcc tgtccagttt tttcatcagg
     2761 gaaaaatcgg gttggcagta ttttacggcg tcttcattag tcagctcgac aaggtattcc
     2821 ctgttctgat aaacaccggt gatttcagga ttgcccaagg aagccagcaa ttctggagga
     2881 tattctgtaa tcttttcagg tggccaggag ggaaaatcca tttccagtat ttttttaccg
     2941 cgggtcaccg tcaataatcc acttttagaa ctgaagatca ctttattctt tttggctttt
     3001 aatatgtcaa agacaagata agctgaagct agtgtcgcat gtccgcataa gttgatctcg
     3061 attgccggtg taaaccagcg gatatcgtaa tctgcttgtt tgtttttaga aggaacaaag
     3121 aaggccgttt cggcaagatt attctccatg gcaatttgtt gcatggtttc atctggcagc
     3181 catttcttca acggaactat tgcggcagga ttgccgccaa atagcttatt ggtaaatgca
     3241 tctgcctgga aaaggtttag tttcatcttt cagttattga tttattataa aattggggca
     3301 aagaccattt aaaccgtacg gccattaccc gaatggtaaa tataattagt atgcaaagaa
     3361 ttttagaggg gactgcatca aatctcattt ccctgagcag gaaataaaaa aggccgcccg
     3421 caatgcaggc cagtgcataa atctctttcc ggaataacag cgggatatta ttcagcagta
     3481 catccctcac cacaccacca aaacaagccg taatagtgcc caatgcaata catatccccg
     3541 tactgaaacc tttatcaact cccagttcaa tgccgattat agtgaaaagg cccaggccaa
     3601 gtgcatcaaa aagaaataag gtaatcgtca gttttttcag gtagcgccca aaaaacatag
     3661 tagcaatagc agaagagaat ataactatag atgctgtttc gttctgcagc cagcttaccg
     3721 gtaagtttcc aatcaaaata tcacgaagag taccacctcc aatggccgta acaaaagaaa
     3781 gcaccagcac tccgaacgga tccagttttt tttccatagc caggaatgcc ccggatactg
     3841 caaaggcgaa tgttcccagt atgtcgatga tgagaagaaa ttgggttggc attgtattat
     3901 cggaaaacat tttatcagta aaaatcccgt tactattcag caacgggacc agctatcttt
     3961 caactctcac tcatcgtttt tgtctttgtt cttttttccc tttttctttt ctttcttctc
     4021 ttcattatct accctgaacg agatatggca aattacgccg taagtgctga tctttccatc
     4081 ctttacatgg gccttcatat cttttaccca tacagaatca atattgcgaa tagttttgga
     4141 gctttcctta acggcttgtt tcactgcatc gtcgaaactt atttccgatg aggcgattac
     4201 ttcgataact tttacaattg gcatagcagt aggtttatgg ttagtgatta tttgtctttg
     4261 tcaagttact atttaattgc acaaatagca acaacagccg gtatatcaaa gtcgctcagc
     4321 ctattctgcc atcatctgcc tgatcacttc ctcaatatcc tcggcattgg gcttactgaa
     4381 ataatccccg tcactgccat aagccggacg atgcgctttt gcggtaatgg ttcgtggggc
     4441 aacatccaaa taacgataac ctccctgttc ttccattacc ttattgaaca tataagctgc
     4501 cgcaccaccg ggcacatctt cgtctacaaa tacgattcga ttggttttct ttaatgactc
     4561 caggatgctg tgattactat caaatggtaa
//
LOCUS       DAORLS010000245         4539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10412_length_4539_cov_2.258252, whole genome shotgun sequence.
ACCESSION   DAORLS010000245 DAORLS010000000
VERSION     DAORLS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10412_length_4539_cov_2.258252"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..>225
                     /locus_tag="PK977_15365"
     CDS             3..>225
                     /locus_tag="PK977_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRF19553.1"
                     /translation="MISSELEIFQDRFKEAVRSHAPLLDRIMRYIVKRKGKQLRPMFV
                     LLSAKLCGEINEPSYRAASLVELLHTATLV"
     assembly_gap    226..250
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <251..951
                     /locus_tag="PK977_15370"
     CDS             <251..951
                     /locus_tag="PK977_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRF19554.1"
                     /translation="MERRGFFSPYALWKAKANVLIGDYLLAKGLLLSLDHNDYQILHI
                     LSDAVRKMSEGELLQLEKARNLNMKEGIYYEIIKNKTASLLASACAAGAWSSCQNEAT
                     TEKLRLFGEKTGMAFQIKDDLFDYASENVGKPTGNDIKEKKLTLPLIYTLNNTDKETR
                     RRIIYIVKNNNNDKEKVKWVIESVEKAGGIDYAKQKMEDFKNEALAVLHEFPESPERK
                     GLEDMVLFVTDRKY"
     gene            976..2682
                     /gene="recJ"
                     /locus_tag="PK977_15375"
     CDS             976..2682
                     /gene="recJ"
                     /locus_tag="PK977_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRF19555.1"
                     /translation="MDKRWNILTADELKTQALQTELKIHPVLCKILVQRGIGTFEQSR
                     NFFRPSLNDLHSPWLMKDMEKAVDRVVRAVQENEKILVFGDYDVDGTTAVASMFLFLQ
                     KIHHNLDFYIPHRYREGYGISKAGIDFAKENGFTLIIALDCGIKSADLIAYAKELDID
                     FIICDHHLPDKILPPALAILNPKQPDCNYPYKELCGCGVGFKLMCALEEKLNLPKGSA
                     MQDLDLLATAIAADIVPVSGENRILAFHGLKKANENPNKGIKALTKLSGLVKELHITN
                     LVFMIAPRVNAAGRMDDASKAVRMFIAKTEDEAMNYAEMLHSDNSDRKEADSSITEEA
                     LALIQENKEWINRKSTVVFKSHWHKGVVGIVASRLIEHYYRPTIVLTRSGDYAAGSAR
                     SVPGFNLYEAIHACREHLLGYGGHFAAAGMTLELGQVDAFREKFEEVVANSIDPKMLI
                     PEIVIDAEIKLDDIRQPFFNILCQMEPFGPENLRPVFITRNVTDTGWSKIVKEDHLKF
                     SVKQNNVTISGIGFNMADKYPLLQEKKPVDIVYKIDENEWQDKKSLQIRVIDVRPASQ
                     RL"
     gene            2728..3948
                     /locus_tag="PK977_15380"
     CDS             2728..3948
                     /locus_tag="PK977_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF19556.1"
                     /translation="MNKALILCIFTLLLSAQVRSQSTASTLDVISWNLEFFGAPYNSG
                     PPDKNLQEINVKKLMRYFNADLYGLLEIVDTMRLRRLVDSLGNTEFGYVIAPYCSNNT
                     TGTGASWTSGQKQAYIYRKSIFSNVSTRGIMRNSSAAYTNWASGRFPFLLSATVTIDG
                     ISKNLNFILLHGKSGSNADDFAKRQAAANELKDTLDAQFSTSNTFIIGDFNDALHNSI
                     YLGSSISSYNTIVADSTDADHYKSVTLPLGAAGQTSMINFPNVIDNHIISNEIVPFYV
                     LGSAQIRTDVTSVVSDYITAHNTSDHYPVFSKYSLAGIITSLPSVTPAELGIKVSPNP
                     FTADVTITASKTLTNVQLTLFNAEGQQLSRQTIGMITAGSSIQPALPVLQKGVYFLQV
                     ETKQYKTTIKLLHL"
     assembly_gap    4026..4036
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4037..>4539)
                     /locus_tag="PK977_15385"
     CDS             complement(<4037..>4539)
                     /locus_tag="PK977_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19557.1"
                     /translation="TNHSATLAGSIAATGCSNVTEYGIEFSGINGFGNGTGTKVVSTN
                     LSSGNYTASLNGLVQGATYYYKAYATNNGGTSYGLQQSFTVASIADGFSIYPAPAQRG
                     ASIRVTKNDLKPGYYGLLFFNSSGQLVYQYNINIQADFINQLITLPGSLMSGAYRVIL
                     VNNDDVI"
BASE COUNT         1419 a          950 c          931 g         1203 t
ORIGIN      
        1 acctgataag tagtgaactg gaaatattcc aggatcggtt taaagaggcc gttagaagcc
       61 atgccccctt acttgaccgg ataatgcggt atatcgtaaa aagaaaaggg aaacagctaa
      121 ggcccatgtt tgtgctcctt tctgcaaaac tttgcggaga aataaatgaa ccttcctacc
      181 gggctgcctc actcgtagag ttgctgcata ctgccaccct tgtacnnnnn nnnnnnnnnn
      241 nnnnnnnnnn cgatggaaag acgggggttc ttctccccct atgctctctg gaaagctaaa
      301 gcgaatgtgc tcataggcga ttatttactg gctaaaggat tactgctgtc gttggatcac
      361 aatgattatc aaatacttca tatactctcc gatgcggtta gaaaaatgag cgaaggggaa
      421 ttacttcaat tggagaaagc cagaaatcta aatatgaagg aaggaatcta ttacgaaata
      481 ataaagaata aaactgcctc cttgttggct tctgcctgtg ctgcaggagc atggagtagc
      541 tgccaaaatg aagccacaac agaaaaactc aggctatttg gcgaaaaaac gggcatggct
      601 ttccaaatca aggatgactt gtttgactat gccagtgaaa atgtaggtaa gcctaccggc
      661 aacgacatta aagaaaaaaa actgaccctg cccctgattt ataccctgaa caacaccgat
      721 aaagaaacac ggagaaggat catttacatt gttaaaaaca ataataatga caaagagaaa
      781 gtaaaatggg ttattgaatc agttgaaaag gccggcggga ttgattatgc caaacaaaaa
      841 atggaagatt tcaaaaacga agccctggcc gttctgcatg aattccctga aagccccgaa
      901 cgcaaaggcc tggaagacat ggtgctcttt gtaacagatc gaaaatacta gtttaaccga
      961 cctttcacct tttctatgga taaaagatgg aatatactca ccgctgatga gctaaagact
     1021 caggccttgc aaactgaact gaaaattcat ccggtgttgt gcaaaatcct ggtacaaaga
     1081 ggcattggta ctttcgagca atccagaaat ttttttcgcc cttccttaaa tgatttgcat
     1141 agcccctggc tgatgaagga tatggaaaaa gcagttgacc gggtcgttag ggccgtccaa
     1201 gaaaatgaaa aaatacttgt attcggcgac tatgatgtag acggtactac tgctgttgcc
     1261 agtatgtttt tatttctcca aaaaatacat cacaatctcg atttctatat cccgcaccgc
     1321 tatagagaag gctatggcat cagcaaagcc ggaattgact ttgccaaaga aaacggtttt
     1381 acattaatca ttgcacttga ttgcggaatt aaatcagctg atcttatagc ttacgcaaaa
     1441 gaactggaca tagattttat catatgtgat caccatttgc cggataaaat tcttccccct
     1501 gcgttggcaa tacttaatcc taaacagcct gattgtaatt atccatataa agagttatgc
     1561 ggctgtggcg tgggattcaa actgatgtgt gcactggaag aaaaactcaa tctgccaaaa
     1621 ggcagtgcta tgcaagatct ggacctgctg gcaacagcca tagcagccga tattgtccct
     1681 gttagcggtg aaaatagaat tctcgctttt catgggctga aaaaagcaaa tgaaaatcct
     1741 aacaaaggca ttaaggcatt aacaaaactt agcggcctgg tcaaagaact gcatatcacc
     1801 aatcttgtct tcatgattgc accaagggta aacgccgctg gcaggatgga tgatgccagt
     1861 aaagctgtac gaatgtttat tgccaaaacg gaagatgagg caatgaacta cgcagaaatg
     1921 ctgcacagtg acaattcaga cagaaaagaa gccgatagca gcatcactga agaagcgttg
     1981 gccttaatcc aggaaaacaa agaatggata aaccgtaaat ccacagtggt tttcaaatct
     2041 cattggcaca aaggtgtagt aggcatcgtc gcctcccggc taattgaaca ttattacaga
     2101 cccacaattg ttctgacccg atcaggagat tatgcagccg gcagtgcccg aagcgtccct
     2161 ggttttaatt tgtatgaagc cattcatgcc tgcagggaac atttgttggg ttacggaggg
     2221 cactttgcgg cagcaggaat gacattggag cttggccagg ttgatgcttt tcgtgaaaaa
     2281 tttgaagaag tggttgcaaa tagtattgat ccgaaaatgc tgatcccgga aattgttata
     2341 gatgctgaaa tcaaactcga tgatatccgc cagccattct tcaacattct ttgccagatg
     2401 gaaccattcg gccctgaaaa cctgcgaccg gtctttatca cccgaaatgt aacggataca
     2461 ggctggtcaa aaattgtaaa agaggatcat ctgaaatttt cagtcaaaca aaataatgtg
     2521 actatttccg gtataggttt taatatggcc gacaaatacc cgcttttaca agaaaagaaa
     2581 ccggtggata ttgtttacaa aatagatgaa aatgaatggc aggataaaaa atcattgcag
     2641 ataagggtga ttgatgtacg tccagcatct caacgcctgt aacagcccgc agatttttga
     2701 cttaccttta tcgcccaaaa attatgtatg aataaagctc tgatactttg catctttaca
     2761 ttgcttctgt cggcccaagt ccgtagtcaa agtaccgcct ccacactgga tgtgataagt
     2821 tggaatcttg aattttttgg agcaccttat aacagcggcc cccctgataa aaatttgcaa
     2881 gaaattaatg ttaagaaact gatgcgctat tttaatgctg atctgtatgg attacttgaa
     2941 atagtagata ctatgcgttt gcgtagactg gttgactctt tgggcaatac ggagtttggt
     3001 tatgttatag ccccttattg tagtaataat acaaccggca caggggcctc gtggacaagt
     3061 ggccaaaaac aggcctatat ctatcgtaaa tctattttct ctaatgtatc cacccgtggt
     3121 attatgcgca acagcagcgc agcttatacc aactgggcca gcggccgttt cccttttctg
     3181 ctttctgcta ccgttacaat tgatggaatc agtaaaaacc tgaattttat tttgttgcat
     3241 ggcaaatcgg gtagtaatgc tgatgacttc gccaaacgac aggctgctgc caatgaacta
     3301 aaagacactt tggatgcaca gttcagcact tctaatacat tcataatcgg tgatttcaat
     3361 gatgccctgc ataatagcat ttatcttgga tccagcataa gctcatacaa tacaattgtt
     3421 gcagacagta ccgatgctga ccattataaa tcagttacac taccactggg tgcagccggg
     3481 caaaccagca tgataaactt tcccaatgta atagacaacc atattatttc aaacgaaatt
     3541 gttccttttt atgtactcgg gtcagctcaa ataaggaccg atgttacttc tgtagtatct
     3601 gattatatca ccgctcataa tacgtccgat cattaccctg ttttttcaaa atacagttta
     3661 gccggtatca ttaccagtct tccttcagtt acacctgctg aattgggaat aaaggtcagc
     3721 cccaacccgt ttactgctga cgttacaata acagcatcca aaacactgac aaacgttcaa
     3781 ctgactttgt tcaacgcaga aggccagcaa ttgagcagac aaactatagg catgataaca
     3841 gccggttctt ctatacaacc ggcattacct gtacttcaaa aaggagttta ttttttacag
     3901 gttgaaacaa aacagtacaa aaccacaatt aagttacttc atttatagaa tcatccttcc
     3961 ggaaataaaa tgggcagcca attggctgcc cattttatta aacattcatg ctgttagaat
     4021 atatgnnnnn nnnnnnctat aacatcatcg ttatttacca gtatcacccg gtaagcccct
     4081 gacatcaatg agccgggcag cgtaatcagc tgattaatga aatcagcctg tatattgata
     4141 ttatactgat aaaccaattg acctgaactg ttgaagaata acaggccata atatcccggc
     4201 ttcagatcat tctttgttac ccttatcgat gcccctctct gagccggagc aggataaatg
     4261 gagaagccat cagcaataga agcgacagta aatgactgct gcaatccata agatgtgcca
     4321 ccattgttgg tagcgtatgc tttataataa taggttgccc cctgtacaag accattgagt
     4381 gaagccgtat agtttccgct gctcaggtta gtagacacaa ctttagttcc tgtgccgttt
     4441 ccaaagccgt taataccact gaattcaatt ccgtactcag taacattgga acaacctgtg
     4501 gcagcaatag atccggccaa agtagcagaa tgattggta
//
LOCUS       DAORLS010000246         4490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10560_length_4490_cov_2.362345, whole genome shotgun sequence.
ACCESSION   DAORLS010000246 DAORLS010000000
VERSION     DAORLS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10560_length_4490_cov_2.362345"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PK977_15390"
     CDS             <1..519
                     /locus_tag="PK977_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19558.1"
                     /translation="AHCCDEVYIFKNTVPGKYQHVAKLFAGNTIITADKQFVFNFYEH
                     FGYFFTCFACAYTDTTDEAPLEVRSIHLKYTKGKLSVVKGDQELRSAINDNLGKLGEQ
                     PYEVLEDEISFDNGLRKEVALNLAVFYYSFGKSLPATKQLFDKYYKFPDARKVWAAFV
                     KNLQEVRTNNDF"
     gene            complement(516..>932)
                     /locus_tag="PK977_15395"
     CDS             complement(516..>932)
                     /locus_tag="PK977_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HRF19559.1"
                     /translation="LELEHDDPFVKDYCGTCTRCIDACPTDAILPEKLIDGSKCISYF
                     TIELKEMLIPGEMKGKFDNWLFGCDTCQDVCPWNRFSKPTTEKDFTALPAILNLSTKE
                     WNEMTEENFKQLFKDSPIKRAKFSGIQRNLKFLGNS"
     assembly_gap    933..952
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<953..1447)
                     /locus_tag="PK977_15400"
     CDS             complement(<953..1447)
                     /locus_tag="PK977_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HRF19560.1"
                     /translation="MQPQKGNTAFIKQTALQLGFDHCGIAKAVKLDDDARRLELWLQN
                     GMHGTMHYIEKYFDLRIDPTKLVPGAKSVITLLKNYFPEKEQQTDGPKISKYAYGKDY
                     HEVIKKQLNDFLSLIKNKVGDIQGRGFVDSAPVLERSWAQRSGIGWIGKNGNLITKQS
                     GSFFF"
     gene            1527..>2371
                     /locus_tag="PK977_15405"
     CDS             1527..>2371
                     /locus_tag="PK977_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRF19561.1"
                     /translation="MKRFIHLILFLVLAIAVAHSQESPKIKFGNVTAADLQHTVYSVD
                     SSADAVVIADIGHTNFVGNMKGTFSLEFKKFKRAHILNKNGYDIADVSISLYTNGQME
                     EDLENLKAVTYNLENGKVVETKLDVKNAVFKDRINKNWVVRKFTFPNIKAGSIIEYEY
                     KVKSDFIFNLQPWEFQGAYPRLWSEYNVRMPEFYTYVTLTQGYQKYYINDRKDSRDNF
                     YMAESRGVGATERASFNAGVTDFRWAMKDVPALKEESYTSTINNHISKIEFQFKSINH
                     PFEYK"
     assembly_gap    2372..2381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2382..>2751
                     /locus_tag="PK977_15410"
     CDS             <2382..>2751
                     /locus_tag="PK977_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRF19562.1"
                     /translation="FMDSWPVVSKQLLNDEDFGLQLRKDNGWLNDVMKAATGGAGSEA
                     DKARNIYNWVRDNFTCTNYNRKYLEQPLKNLLKSKNGNEAEINLLLTAMLLKADLKAD
                     PVMLSTRSHGYAHAVYPLMER"
     assembly_gap    2752..2775
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2776..3549
                     /locus_tag="PK977_15415"
     CDS             <2776..3549
                     /locus_tag="PK977_15415"
                     /inference="COORDINATES: protein motif:HMM:NF024372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3858 domain-containing protein"
                     /protein_id="HRF19563.1"
                     /translation="KVKIDGKDVFLDASHPELGYGHLNYDCYNGQARVIDNDATVIEM
                     DAGSLKEKSVISLFIINDEKGNSVGSLQKAPGYYESLRIRSKVKEKGKDNYFAELKKS
                     FGTEIKMSNERIDSLTKLDDPVGVFFDFDFTGEKEDIIYFNPMLIEGYKENPFKSAKR
                     YYPVEMPFALDETYNMQMEIPTGYVVDELPKQMVMKMNEEGDGTFEYRVSVSGNNISF
                     RSRLVINRTFYLPEEYEMLREFFNLVVKKHNEQIVFKKK"
     gene            3554..>4490
                     /locus_tag="PK977_15420"
     CDS             3554..>4490
                     /locus_tag="PK977_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRF19564.1"
                     /translation="MKKWVIGILTNLFVLSLLAGEGEYAVSKINPLLLKNANAVLRLE
                     ELKFEVSATGKATYKNHYVITILNENGDHWAECYEYYDKYRKIESVEGFLYDAGGNQL
                     KKMKLKEMEDLSGVSAISLMEDDRVKRHNFYHKVYPYSIEYNIVIDYNSTFFFPPWVP
                     ASRSNLAVESSSMSMVCKDGYQFRYKAFNYPGEPVVTTEKDTKITTWTAKNISAIVKE
                     PFAPMWHELTTMVIFGPTDFQVDSYKGNMRSWQDFGKFQYTLLQGRDQLPDNVKQDVH
                     RLTDAVTDKNQKIALLYDYLQKNTRYISIQLGIGGW"
BASE COUNT         1356 a          857 c          973 g         1250 t
ORIGIN      
        1 gcccattgct gtgatgaagt atatatattc aaaaatactg taccaggaaa ataccagcat
       61 gtagctaaat tgtttgctgg taacactatt attacagccg ataaacaatt tgtgttcaat
      121 ttctatgaac actttggtta tttcttcacc tgttttgcct gcgcttatac tgacacaacc
      181 gatgaagcgc ctttggaagt cagaagcatt cacctgaaat atacaaaggg aaaattatca
      241 gttgtaaaag gagaccagga gctgcgcagc gccattaatg acaaccttgg caaactaggt
      301 gagcagcctt atgaagtctt agaggatgaa atctcttttg acaatggcct tcgcaaagaa
      361 gtggccctga acctggcagt tttttattat tcattcggca aaagcctgcc ggctaccaaa
      421 caacttttcg ataagtatta taagtttccc gatgcccgca aggtatgggc agcttttgta
      481 aaaaacctgc aggaagtaag gactaataac gatttttaag aattcccgag aaatttcaag
      541 ttgcgttgta taccgctgaa ttttgccctt ttgatcggcg agtctttaaa tagttgttta
      601 aaattttctt ctgtcatctc attccattcc ttcgtggata ggttcaatat tgcaggcaga
      661 gcagtaaaat ctttttcagt agttggtttg ctaaaacggt tccaaggaca aacatcctgg
      721 caggtatcac aaccaaaaag ccagttatca aacttccctt tcatttcgcc agggataagc
      781 atttctttaa gctcaatggt aaagtaggag atgcacttac ttccgtcaat cagtttttca
      841 ggaaggatgg catctgtagg acaggcatct atgcagcggg tgcaggtgcc acagtaatct
      901 ttaacgaacg gatcatcatg ctccagttca agnnnnnnnn nnnnnnnnnn nnaaaaaaga
      961 atgagccact ttgtttggtg atcaggttgc cgttttttcc tatccagcca atgccgctgc
     1021 gttgtgccca gcttctttcc agaaccgggg cagaatccac aaatcctctt ccctgtatat
     1081 ctccaacttt atttttaatt agcgacaaaa aatcattgag ttgttttttg atcacttcat
     1141 gataatcttt tccgtatgca tattttgaaa ttttggggcc atctgtttgt tgttcttttt
     1201 ccggaaaata atttttaagt aaagtgataa cagattttgc gccgggaacc agtttggtcg
     1261 ggtcaattcg caggtcaaaa tatttttcta tgtaatgcat ggttccatgc atgccatttt
     1321 gcagccatag ttccagtctc ctggcatcat catccagttt gacagctttg gctatgccgc
     1381 aatgatcaaa gcccagttga agagctgttt gttttataaa ggctgtattt cctttctggg
     1441 gctgcaattt ttttggtgtt tgctcagcag caaattcagg atttttatgt aaaattgatt
     1501 gattatttta ccaacacaca cctgctatga aaagattcat tcaccttatc ctctttttgg
     1561 tattggcaat agcggttgct cattcccagg aatcacccaa gatcaaattt ggtaatgtta
     1621 cggcggcaga cctgcagcac actgtttatt cggttgacag cagcgcagat gcggttgtca
     1681 tcgcagatat tggtcacacc aattttgtgg gaaacatgaa aggaactttt tcccttgagt
     1741 tcaagaaatt taaaagggca cacatactga ataaaaatgg ttatgatatt gcagatgttt
     1801 ccatcagttt atacaccaac ggccagatgg aagaagacct ggagaacctg aaagcagtta
     1861 cctacaacct ggagaacggt aaagtggtgg aaacaaagct ggatgttaag aatgcagtat
     1921 ttaaggacag gatcaacaaa aactgggtag taagaaaatt cactttcccc aatatcaagg
     1981 ccggttctat tattgaatat gaatacaaag tcaaatctga ttttattttc aacctgcagc
     2041 catgggagtt ccagggtgct tatccccgtt tgtggagtga atacaatgta aggatgccgg
     2101 agttttacac ctatgtaacg ctgacacagg gttaccagaa atattacatc aacgaccgca
     2161 aagacagccg ggacaatttt tatatggcgg aaagccgtgg ggtaggtgct acggaaaggg
     2221 cttcgttcaa tgcaggtgtt accgattttc gctgggccat gaaagatgtg ccggcgttga
     2281 aagaagaaag ctacacttct accatcaata atcacatttc caagatcgag ttccagttca
     2341 aatcgatcaa tcacccgttt gagtacaaaa annnnnnnnn ntttatggac tcatggccgg
     2401 ttgtttctaa acaattgctg aatgatgagg actttggttt gcaattaaga aaagataacg
     2461 gctggctgaa cgatgtgatg aaagcagcaa ccggcggtgc cggcagtgaa gccgataaag
     2521 cccggaatat ttataactgg gtgagagata attttacctg tacgaattat aaccgtaagt
     2581 atttagagca gccattgaaa aacctgctaa agtcaaaaaa cggaaatgaa gcagagatca
     2641 acctgctgtt gacagccatg ctcctgaagg cagacctgaa agccgatccc gttatgctga
     2701 gtacccgttc gcatgggtat gcccatgcag tctatccgct tatggaacgc tnnnnnnnnn
     2761 nnnnnnnnnn nnnnnaaagt aaagattgat ggcaaggatg tatttctaga tgccagtcat
     2821 cctgaactgg gttacggcca tttgaattat gattgctaca acgggcaggc aagagtaatt
     2881 gataatgacg caaccgttat tgaaatggac gctggctcat taaaagaaaa atcagttatc
     2941 tccctgttca tcatcaatga tgaaaaagga aattctgtcg gcagcctgca aaaagcacct
     3001 ggctattatg aatccttacg catacgcagt aaggtaaaag aaaaaggaaa ggacaactat
     3061 tttgctgagc tgaaaaagag ttttggaact gaaataaaaa tgagcaatga aagaatcgac
     3121 tctcttacta agctggatga tcctgtaggt gtttttttcg attttgattt tacaggcgaa
     3181 aaggaagata tcatttattt caaccccatg ctgatcgaag gttacaagga aaatcccttt
     3241 aaatcggcta aacgttatta ccctgttgaa atgccttttg cattggatga aacttacaat
     3301 atgcagatgg aaatacccac cgggtatgtg gtagacgaac tgcccaagca aatggtgatg
     3361 aagatgaatg aagaaggaga cggtacgttt gaatacaggg tatctgtttc aggcaataat
     3421 atttctttcc ggtcaaggct ggtgatcaac cggactttct atttaccgga ggaatacgaa
     3481 atgctgagag agtttttcaa cctggtagtg aaaaaacaca acgaacaaat tgttttcaaa
     3541 aagaaatagt gatatgaaaa agtgggtgat tggcatacta acaaatcttt ttgtcctttc
     3601 tctgcttgca ggagagggag aatatgcagt atcaaagatc aatccgcttt tgctgaaaaa
     3661 tgccaacgcc gtgttgcggc tggaagaatt gaagtttgaa gtgtccgcta cgggtaaagc
     3721 cacttacaaa aatcattatg ttattacaat actcaatgaa aacggcgatc attgggccga
     3781 atgttatgag tattacgata agtaccggaa aatagaatcc gttgaaggct ttttgtacga
     3841 tgccggtggt aaccagttga agaaaatgaa actaaaggaa atggaagacc tcagcggcgt
     3901 ttcggctatt agcctgatgg aagatgaccg ggtgaagcgc cataatttct atcacaaagt
     3961 atatccttac tccatagagt acaatattgt aattgactac aacagcactt tctttttccc
     4021 tccctgggta cctgcttcaa gaagtaatct tgctgtcgag tcaagcagca tgagcatggt
     4081 atgtaaagat ggttaccagt tccgctataa agcctttaat taccccggcg aaccggtggt
     4141 aacaacggaa aaagatacaa agatcactac atggaccgct aaaaatattt cggccattgt
     4201 aaaagaaccc tttgccccta tgtggcatga actgaccacc atggttattt ttggtcccac
     4261 tgacttccag gtggacagct ataagggcaa tatgcgcagc tggcaggatt ttggaaaatt
     4321 ccaatatact ttgttacaag gaagagacca gcttcctgac aatgtaaaac aggatgtgca
     4381 caggctgaca gatgcagtga cggataaaaa tcagaagatc gctttgctgt atgattattt
     4441 gcagaaaaac acaaggtata tcagtataca gctaggtatt ggcggctggc
//
LOCUS       DAORLS010000247         4482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10588_length_4482_cov_2.175062, whole genome shotgun sequence.
ACCESSION   DAORLS010000247 DAORLS010000000
VERSION     DAORLS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10588_length_4482_cov_2.175062"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK977_15425"
     CDS             complement(<1..216)
                     /locus_tag="PK977_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19565.1"
                     /translation="MVKLDAGTTFQRLRNRYRLVVMNDDTYEEVVTFKLSRLSVYIML
                     STIFVLLVGLTVALIVFTPLKYYIPGAS"
     gene            360..>3146
                     /locus_tag="PK977_15430"
     CDS             360..>3146
                     /locus_tag="PK977_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19566.1"
                     /translation="MRLNRYTTTAILLLILCQVSFPAFSQLGISFDIKKPKEYDERVL
                     RSEKSDQKKFPRPRRFIQNTATHYNYFFNANNKLNEVLERAKEAYKDDYSKLLPFYNY
                     SLDATAADSIQLDSITYKASTGIALHDLRSDWVDNLYLLWGAAFYLQKKFDSAYLMFQ
                     FINYAFAPKEKDGYYLTIGSARDGNSAYSISTKEKRSLPKRLLSEPPSRNDAFIWQIR
                     NFLAQDQFAEAASLIVTLRNDPNFPKRLVNDLHEVQAYWFYKQNMWDSAANHLVSALS
                     NAGNQQEKARWEYLAAQLYELAGQYKEAEDYYSKVSSHTTDPILDIYARLASIRVNRD
                     DDKTIEKNIAALIRMAKQDKYEDYRDIIYYMAAQMQLEGKDIDGAMALLLKSTQFATN
                     GDAQRNKAFLQLAELAFAQKKYRLSYNYYDSIRLGDPTLKNPDEIKARKESLAVIALN
                     YEILERQDSLQRIAALPEQERKDFVKKLVKQLRKEKGLKEEQPASAASPFATTAPPPL
                     FPSGDSKGEWYFYNEASRTRGTTDFKARWGVRPNVDNWRRSAVLAGIINSNQPGNQNQ
                     AGGKPTQGGGDDELSFEGLYNKLPITPELLSLSNDSVQQALFELGKAYIQNIEDCNAG
                     TETLEQLRSRFPQFAKMSEVLFNLYYCYNKNGNSARAAEVKELLTNNYGMTSFAKILA
                     TGENPDAKTPGNAATRTYEKIYDLFIEGKFAEAIAQKKTADSIYGRNYWTPQLLYIEA
                     VYYIKQRQDSIAKDVLTNIIGQFANTPIAAKAATLLDVLGRRAQIEDELKRMVVTRAP
                     SDTAAGRQPVTIIQPPKPKTDTATTKPPVVTVPVKPVTDSSQIKPAVKPDAPFVHAPA
                     EAHYVVLILNKVDPVFSNEAKNAFARYHRDNYYNKQFSLDLFELDADNRLLLIAPFTN
                     AQDAIAYIDKTKPRT"
     assembly_gap    3147..3166
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3425..>4482
                     /locus_tag="PK977_15435"
     CDS             3425..>4482
                     /locus_tag="PK977_15435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRF19567.1"
                     /translation="MKKAVRIFWRIFFGGFAAFILLIILANFGVFGKMPSLTDLENPS
                     ILQASEVYAEDGTLMGKYYTERGNRSNVKYKDISRHVVDALVATEDERFYSHSGIDFK
                     STLRAIFTLGSQGGGSTITQQLAKALLDQGSKNKAWRVIEKLKEWIIAVKLEKNFTKE
                     EIITLYLNAVPFGNNIYGIRNAARTFFQKEPDRLNVEEAALLIGMLKGNSIYNPIRNP
                     KASLDRRNVVLSQMEVNGKITPAVAAKLKASPIKLNYKKLDENTGYAPYFREVLKSEI
                     KEALKDVKKNNGDTYDIYDDGLKIYTTINVQMQEYAEEAMAQHMATLQKSINARQDMK
                     TGSIWKGREKFLEKAIKES"
BASE COUNT         1454 a          969 c          925 g         1114 t
ORIGIN      
        1 gctggccccc ggaatataat acttcaaagg tgtgaaaacg atgagggcta ctgtcagtcc
       61 aaccaataac acaaatattg tgctcaacat aatataaaca ctcaaacggg acaatttaaa
      121 ggtcaccacc tcctcataag tgtcatcatt catcacaacc agcctgtagc ggtttctaag
      181 ccgctgaaag gtggtaccgg catctaattt taccataacc aaaaatttgg ggactaaagg
      241 taaggattgg ttattggttt tggggtattc aaaggggcaa tttctgttga aaaaaatggc
      301 ctagatttat actaaaatat ttgaatttca gttattaaga ctttagattg cgccggaaaa
      361 tgcgattgaa ccgatatacg actactgcga tcctcctcct tatcctctgc caggtcagtt
      421 tccccgcttt cagccagttg ggaatctctt ttgacatcaa gaagcccaag gagtacgatg
      481 aaagggtgct gcgatctgaa aaatcagatc agaagaaatt tccccggccc cgccggttca
      541 ttcaaaacac cgccacgcat tacaattatt tcttcaacgc caataacaag ctgaacgaag
      601 tgctggaaag ggccaaagaa gcctacaaag acgattactc caagcttctc cctttttata
      661 actattctct tgacgctaca gcggcagatt ctatacaact tgattccatt acgtacaaag
      721 cctcaacagg tattgccctt catgacctgc gcagtgactg ggtagacaac ctgtatcttt
      781 tatggggagc cgctttttac ctgcaaaaga aattcgattc tgcctacctg atgttccagt
      841 ttattaacta tgcttttgcc ccgaaagaaa aggatggtta ttacctgacg atcggcagtg
      901 ccagggatgg caattcggct tacagtattt ctacaaaaga aaaaagaagc ctgcccaaaa
      961 gattactttc agaaccgccc agccgcaatg atgcctttat atggcaaata agaaatttcc
     1021 tggcccagga ccagtttgcc gaagccgcca gtttgatcgt aacgcttcgc aacgacccta
     1081 attttcccaa acgcttagtg aatgacctgc acgaagtaca ggcctattgg ttttacaagc
     1141 agaacatgtg ggacagcgct gccaatcacc ttgtcagtgc tttaagcaat gccggtaacc
     1201 agcaggaaaa agcccgttgg gaatacttag ccgcacaact ctatgaactg gccggccaat
     1261 acaaagaagc agaagattat tacagcaagg taagcagcca tacaaccgat cccattttag
     1321 acatttatgc ccgcctcgcc tctattcggg taaaccgtga tgatgataaa accatagaaa
     1381 aaaatattgc tgcccttatc cgcatggcaa agcaggataa atatgaagat taccgggata
     1441 ttatttacta catggcggca cagatgcaac tggaaggaaa agatattgac ggcgccatgg
     1501 cattactcct gaaaagtacc cagtttgcca ctaacggtga tgcccagcgc aacaaagctt
     1561 ttcttcaact ggcagaactg gcatttgcac aaaaaaaata caggctttca tataattatt
     1621 acgacagtat ccgtctcggc gaccccactc tgaaaaatcc tgatgagata aaagcacgga
     1681 aagaatctct tgcagttatt gctctcaatt atgaaatact ggaaagacag gacagccttc
     1741 agcgtattgc tgccctgcct gaacaagaaa gaaaagattt tgtaaagaag ctggtaaaac
     1801 aattgcgcaa ggaaaaaggg ctgaaagaag aacagcctgc ttctgcagct tctccttttg
     1861 ccactaccgc acctcctcct cttttcccat ccggggattc aaaaggagaa tggtatttct
     1921 ataacgaagc ttccaggaca agaggaacaa ctgatttcaa agcaagatgg ggtgttcgtc
     1981 ccaatgtaga taactggcga agaagtgcag tattggccgg tatcatcaac agcaaccaac
     2041 ccggtaatca aaaccaggca ggtggaaaac ccacacaagg tggaggagat gatgaactct
     2101 cctttgaggg tttgtacaac aaactaccga taacacctga acttttgtcg ttgtccaatg
     2161 attctgttca gcaggcatta tttgaactgg gcaaagctta tattcagaat attgaggatt
     2221 gtaatgcagg cactgaaacg ctggaacaat tacgcagtcg cttccctcaa tttgccaaaa
     2281 tgagcgaagt attgttcaac ttgtattatt gctataataa aaatggtaat tcagccaggg
     2341 cagcagaagt aaaggaattg cttaccaaca attatggcat gacttctttt gcaaagatct
     2401 tagctaccgg tgaaaaccca gatgccaaaa ctccgggtaa tgctgctacc agaacatacg
     2461 aaaagatcta cgacctgttt attgaaggaa aatttgctga ggccattgcg caaaagaaaa
     2521 ctgccgacag catttatgga aggaattact ggacaccgca actgctttat attgaagcag
     2581 tatattacat caagcaaaga caggacagta tagccaaaga tgtactcacc aatataattg
     2641 gacaatttgc caatactcct attgcagcca aagcagctac cttactggat gtgctgggcc
     2701 gcagggcaca aatagaagat gaattaaaaa gaatggttgt aacccgggcg ccatcagaca
     2761 ctgcagcagg acgtcagccc gttacaatta ttcagcctcc aaaaccaaaa acagatacgg
     2821 ctactacaaa accaccggtt gtaactgttc ctgttaaacc ggttacagat tcttctcaaa
     2881 taaaacctgc agtaaaacct gatgcacctt ttgtacatgc tcccgccgaa gcccattatg
     2941 tggtgctgat attaaataag gttgacccgg ttttctcaaa tgaagccaaa aatgcatttg
     3001 cccgttatca cagggataat tattacaata agcagttttc tttagactta tttgaattgg
     3061 atgcagataa caggttattg ctgatcgccc cgttcaccaa tgcacaggat gccattgctt
     3121 atattgacaa aaccaaaccg agaacgnnnn nnnnnnnnnn nnnnnncatt ccgtggctca
     3181 agggaggaaa atacaatttc agcatactta ccaatggtaa ttttgacctg ctgaaacaaa
     3241 acaaaaacct cgaaggatac agaaacttcc tgaaccagta tttccccggt aaattttaac
     3301 gggaagcccg gttaattatg tttgttttgg caaaaaatta atgactgtgc ccttccagcc
     3361 gcataggttt ttcttaattt cgaatccggt ttattaaacc atttttatca caatcctcaa
     3421 ctaaatgaag aaagctgttc ggatcttctg gcgaatattc tttggtggtt tcgctgcatt
     3481 tatcctgttg atcatactgg ccaattttgg tgtatttggg aaaatgccct cacttactga
     3541 cctggaaaac ccttccattt tacaggcttc tgaagtatat gctgaagatg gcaccctgat
     3601 gggtaaatac tatacagaaa gaggaaacag gagtaatgtg aagtacaagg atatatcccg
     3661 ccatgttgtg gatgccctgg tagctacaga agatgaacgt ttttatagtc acagcggcat
     3721 tgattttaaa tcaaccctta gagccatttt caccctgggc tcgcagggag gaggcagtac
     3781 cattactcaa caattagcaa aagcattact ggaccaggga agcaagaaca aggcatggcg
     3841 ggttattgaa aaattaaaag agtggatcat agctgtcaaa ctggaaaaga attttaccaa
     3901 ggaagagatc attacgctgt acctcaatgc tgtgcctttc ggaaacaata tttacggtat
     3961 ccgaaatgca gcgagaacat tctttcaaaa agaaccggac aggctgaatg tggaagaagc
     4021 ggctctgctt attggaatgc taaagggtaa cagcatctac aatcccatca ggaaccctaa
     4081 agcttcattg gaccggagaa atgtggtact cagtcaaatg gaagtgaacg gaaagataac
     4141 acctgccgtg gctgctaaat taaaagcaag tcccataaaa ctgaattata aaaaactgga
     4201 tgagaacact ggttatgctc cctactttcg ggaagtttta aaaagtgaaa taaaagaagc
     4261 ccttaaagat gttaagaaaa ataacggcga cacatacgat atatatgatg atggcctgaa
     4321 aatttatacc actattaatg tacaaatgca ggaatatgcc gaagaagcaa tggcccagca
     4381 tatggccaca ctgcaaaaaa gcatcaatgc aagacaggac atgaaaaccg gttctatctg
     4441 gaaaggacgg gaaaaatttt tagaaaaagc cattaaagaa tc
//
LOCUS       DAORLS010000248         4439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10750_length_4439_cov_1.835538, whole genome shotgun sequence.
ACCESSION   DAORLS010000248 DAORLS010000000
VERSION     DAORLS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10750_length_4439_cov_1.835538"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>231)
                     /locus_tag="PK977_15440"
     CDS             complement(<1..>231)
                     /locus_tag="PK977_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRF19568.1"
                     /translation="VAVVLIPTLGDYSPEEVGLEIIRTWGVGNKNTNNGIVILVAKND
                     RKVRIEVGYGLEGAIPDITAKSIIDNEITPNF"
     assembly_gap    232..325
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(428..886)
                     /locus_tag="PK977_15445"
     CDS             complement(428..886)
                     /locus_tag="PK977_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRF19569.1"
                     /translation="MFSLFKKNKQLFPEEDTRLIVKAIRQAEKSTSGEVRVFVENRCS
                     WMDALDRAAELFFGLKMDKTEQRNGVLVYIALKDHQLAVFGDEGIHRKVGSEYWNNLV
                     KEMLASFNKHDYAKGIAECVIQIGDALQTHFPYERDTDKNELPDEIVFGR"
     gene            complement(901..1497)
                     /locus_tag="PK977_15450"
     CDS             complement(901..1497)
                     /locus_tag="PK977_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRF19570.1"
                     /translation="MKGTRNLSLLIIGGLVLILFAWGCNGYNGLVKGDQNVKKVWSNV
                     ETNYQRRTDLYNSVIKTIEGSANFEKSTLKDVLDARARATSVNVDINDPASLEKYQQA
                     QGSLQSAFGRLLAVVENYPDLKTTKAFQDFQTQIEGTENRINVARQDYNKSVEDYNLK
                     VKRFPNNLLAGIFNFKEKAYYKADPGSEKNPEIKFDIK"
     gene            complement(1686..2882)
                     /locus_tag="PK977_15455"
     CDS             complement(1686..2882)
                     /locus_tag="PK977_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRF19571.1"
                     /translation="MASFANYDFKGQKALIRVDFNVPLNDKFEITDDTRMRAAVPTIK
                     KILADGGMVILMSHLGRPKDGPTEKYSLKHLITHLSQLLGGTRVLFANDCIGEQAYLT
                     ASMMRPGEVLLLENLRFYKEEEKGNEAFAEKLSKLGDVWVNDAFGTAHRAHASTAVIA
                     KFFPKEKRLFGLLMEGEVSSAEKVLHQAEKPFVAIIGGAKVSDKILIIENLLERATDI
                     IIGGGMAYTFMKARGGKIGNSLCEEDRLSTALEILQKAKDRNVSIHLPEDSVIADKFD
                     ANANTDTAPSDAIPDGWMGLDIGPKAEGIFAEVIRKSKTILWNGPMGVFEMEKFQKGT
                     KSIATAIAEATQNGSFSLVGGGDSVAAVNQFGFADKVSYVSTGGGAMLEYFEGKTLPG
                     IAAVFE"
     gene            complement(2900..3274)
                     /locus_tag="PK977_15460"
     CDS             complement(2900..3274)
                     /locus_tag="PK977_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19572.1"
                     /translation="MKIKNAENLSAEQLQEEVNRGGRFVHYQYAVSFIVAAIKPDTGV
                     YLIRKGESAVKESLFFTILSLLFGWWGIPFGPKHTIAAIRTNLRGGKDVTDEVVATIA
                     GHILFREAEKRKAKARVLEPIS"
     gene            complement(3340..4347)
                     /gene="gap"
                     /locus_tag="PK977_15465"
     CDS             complement(3340..4347)
                     /gene="gap"
                     /locus_tag="PK977_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRF19573.1"
                     /translation="MTIKIGINGFGRIGRLVYRQIYKMEGIDVVAINDLTSPKVLAHL
                     LKYDSAQDRFDAEVKATEDSIVVNGDTVKIYAQKNPADIPWKSHDVDVVLECTGFFTD
                     KDKATAHITAGAKRVVISAPATGDLKTVVYNVNHSILDGTETIISCASCTTNCLAPMA
                     KVLNDNFGIETGIMTTIHAYTNDQNTLDAPHAKGDLRRARAAAANIVPNSTGAAKAIG
                     LVLPELKGKLDGGAQRVPTITGSLTELTSILSKPVTKEEVNATMKAAANESFGYTEDE
                     IVSSDIIGIHYGSLFDATQTKVMTVGDKQLVKTVSWYDNEMSYVSQLVRTVKYFAKLI
                     S"
BASE COUNT         1106 a         1042 c          877 g         1320 t
ORIGIN      
        1 ttgaaattgg gagttatttc gttatcaata atgcttttgg cagttatgtc gggtattgct
       61 ccttccagcc cgtagcctac ttctattctt actttccggt cattctttgc tacaagtatt
      121 acaattccat tattagtgtt tttattaccc actccccagg tacggataat ttcaagacct
      181 acttcttccg gagaataatc acccagtgtt ggtatcaaaa caactgctac cnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnagctt tggagggctc ggtcttgggg gaacaatctt
      361 gtcaagctgt gcgaaagcac ccaaagacaa gaacaaccct attatgagta agattttttt
      421 cattcagtta tctgccaaag acaatttcat cgggcagttc atttttgtca gtatcccttt
      481 cgtatgggaa atgtgtttga agggcatcgc ctatctgtat aacacattcg gcgatgcctt
      541 ttgcataatc atgtttatta aaactggcca gcatttcctt taccagatta ttccagtact
      601 cagaaccaac tttccggtga atgccttcat cgccaaatac tgccagttga tgatccttta
      661 aagcgatata aaccaatacc ccattacgct gctctgtttt atccatttta agaccaaaaa
      721 acaactcagc agccctgtcc agcgcatcca tccagctgca gcgattttct acaaataccc
      781 ttacttcacc gctggtgctt ttctccgcct ggcggattgc ttttacaata agccgggtgt
      841 cctcttccgg gaagagttgt ttgttctttt tgaataagga gaacatatac agttcaggta
      901 ttacttaata tcaaacttta tttcgggatt cttttctgaa cccggatctg ctttgtaata
      961 agctttctcc ttgaagttga agattccggc caacaggttg ttggggaagc gttttacctt
     1021 caggttgtag tcttcaacag atttattgta atcctggcgg gccacattga tgcgattctc
     1081 ggttccctca atttgtgttt gaaaatcctg gaacgctttg gtggttttta aatcaggata
     1141 gttttctact acggctagta aacgaccaaa agcactctgc agactaccct gagcttgctg
     1201 gtatttctcc agtgaagcag gatcattgat atctacattt actgaagtag cccttgcccg
     1261 tgcatccagc acatccttca aagtggactt ttcgaaattg gcagatcctt caattgtttt
     1321 tataacactg ttgtacaagt cagttctgcg ctggtagttc gtttccacat tactccagac
     1381 tttttttaca ttctgatcac ctttgaccaa accgttgtat ccattacagc cccaggcgaa
     1441 gaggatgagg acaagaccac cgatgatcag taaggataag ttgcgggttc ctttcatttt
     1501 gttatggttt tattgattat taaaattacg ggtttacaaa caaaaagctc ctgagcaaag
     1561 caggagccaa ataatatttt atgccaaaat agcgggaaag acaggattcg tccaaactca
     1621 acaggcttct atttacgact cacctaagta tctcagtcaa aacaagctta aagcctatcc
     1681 tgcgcttatt cgaaaacagc agctattccg ggcaatgtct ttccttcgaa atattccagc
     1741 atagcgccac cacctgtact gacgtagctt actttatccg caaagccaaa ctggttaact
     1801 gctgctactg agtcgccgcc gccaaccaga gaaaaagaac cattctgtgt agcttcagca
     1861 atagctgtgg caattgattt ggttcccttc tggaattttt ccatttcaaa cacacccatg
     1921 gggccgttcc aaagaatcgt tttagacttt cgtatcacct cggcaaagat gccttctgct
     1981 ttagggccaa tatccagccc catccatcca tcaggaatag catcactcgg ggctgtatct
     2041 gtattggcgt tggcgtcaaa cttatctgca atcaccgaat cttccggtaa atgaatactt
     2101 acattacgat cttttgcttt ttgcaatatt tccaatgcag tagataaccg atcctcttca
     2161 catagtgaat tacctatctt acctcctctt gctttcataa aggtataggc cataccgccc
     2221 ccgataatta tatcggttgc cctttcgagc aggttttcga tgatgaggat cttgtcgctc
     2281 actttagcgc ctccgatgat tgccacaaaa ggtttttcgg cctggtgcaa gactttttca
     2341 gcactggata cttcgccctc catcagcaag ccaaataatc ttttctcctt aggaaaaaac
     2401 ttagctatta ctgctgtgga ggcatgtgcc ctgtgtgcag tcccaaacgc atcatttacc
     2461 catacgtcgc ccagtttgga tagtttttct gcaaaagctt cgttaccctt ttcctcttct
     2521 ttataaaaac gaaggttttc taatagcaac acttctcccg ggcgcatcat gctggctgtt
     2581 aggtatgcct gctcacctat acaatcgttg gcgaaaagca ccctcgtacc acccagcaat
     2641 tggctgaggt gagtgatgag gtgtttcaag gaatattttt ctgttggccc gtctttggga
     2701 cgaccgaggt ggctcatcag aatgaccatg ccgccatctg ctaagatttt tttgatggta
     2761 ggaacggcag cccgcatgcg ggtatcatcg gttatttcaa atttatcatt cagaggaaca
     2821 ttgaaatcta cacgaatcaa ggctttctgg cctttaaaat cgtaattggc aaaactggcc
     2881 atatataaat gttttagcgt taactaatag gttcgagtac cctggcctta gcctttcttt
     2941 tctccgcttc acgaaaaaga atatgaccgg cgattgtagc caccacttca tcggtaacat
     3001 cttttccacc ccgaagattg gtgcgtatgg cagcaatagt atgtttgggt ccgaaaggta
     3061 ttccccacca gccgaacaaa agagaaagaa tcgtaaagaa gagactctct ttcacagcac
     3121 tttctccttt gcgaataagg tacacccccg tgtcgggctt tatagctgca acgatgaaag
     3181 aaacggcata ttggtaatga acaaatcttc caccccggtt cacctcttcc tgcaactgtt
     3241 ctgcagaaag gttttcagca tttttaattt tcattcggaa aaagagagca acgcagcaag
     3301 tgttgccacc gctgcattgc aggattaagg ttaatcgatt taactgataa gctttgcgaa
     3361 gtatttcact gtgcgcacca gctgacttac ataactcatt tcattatcgt accagctaac
     3421 ggtcttcacc aattgcttgt cgcctacagt cattactttg gtctgcgtag catcaaacag
     3481 cgaaccataa tgaataccaa tgatatcact gcttacgatt tcgtcctctg tataaccaaa
     3541 agattcgttt gccgcagctt tcatggttgc atttacttct tctttagtaa ccggcttgct
     3601 gaggattgat gtaagctcag tcagtgatcc ggtaatagta ggcacccgtt gtgcacctcc
     3661 atccaatttg cccttcagtt ctggcagtac aaggcctata gctttggcag caccggtgct
     3721 gttaggaaca atattggctg ctgctgccct tgccctgcgc aagtcgcctt tcgcatgagg
     3781 ggcatccagt gtgttctggt cgttggtgta agcatgaata gtcgtcataa tacctgtttc
     3841 gataccaaaa ttatcattca gtactttggc cattggcgcc aggcagttag tggtgcatga
     3901 agcacatgaa atgatagtct cggttccgtc gagaatgcta tgatttacat tatacactac
     3961 tgttttcagg tcgcctgttg caggtgccga gattaccact cttttggccc cggctgtgat
     4021 atgggccgtt gctttatcct tgtcggtgaa gaaaccggta cactccagca ctacgtccac
     4081 atcatggctt ttccagggaa tgtcggcggg gtttttctgg gcatatattt ttacagtatc
     4141 tccgtttact acgattgaat cttccgtagc ttttacttct gcatcgaagc gatcctgtgc
     4201 actgtcatac tttaagagat gggccaaaac tttcgggctg gtcaggtcat tgatggccac
     4261 cacgtcaatt ccttccattt tatatatctg gcgataaact aaacggccga tacgtccaaa
     4321 accattgatg ccgatcttga tggtcatgaa acagtctcct tgaaaaatta cagaagtgat
     4381 ctcacggttg ccaccacgcg gcgagccgtg aaaccaaatg cgtcaaacaa ctggccggc
//
LOCUS       DAORLS010000249         4437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10758_length_4437_cov_1.916476, whole genome shotgun sequence.
ACCESSION   DAORLS010000249 DAORLS010000000
VERSION     DAORLS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10758_length_4437_cov_1.916476"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK977_15470"
     CDS             <1..485
                     /locus_tag="PK977_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19574.1"
                     /translation="PEADPLRNTYIQHTKLKKMINKIPAKQVLVLLDICHGGVFDENV
                     LGKTRDNPAAAITNRNVLELLKDKSQYVTRKVLSSVGKESAFDGRAGKHSPFAAYLLK
                     ILNARGGAEGIVTLSDIYALLQKASLNETETLKISPHLAGFGKNDPQSEFILIPVEKK
                     "
     gene            complement(489..>2347)
                     /locus_tag="PK977_15475"
     CDS             complement(489..>2347)
                     /locus_tag="PK977_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF19575.1"
                     /translation="GKEYKILSCQPNQLSLESEEWGGGRGLFSLHLEEGMKGLADRNN
                     DGRISFSELDRYIRDKVETFSEDKQIPLIVGDLSKSFVNVNPAVLAALKIQKAKERPA
                     LAQVNPKGVEEKYVDSLDPAGKKLYASYKKNLSDRKLIWPLDTNALKDYKAFAKKYND
                     NPLTPLMRRNLAAALNQRFDSIVSPLLRGETSFSTRDMCYYASMELDSCMQLLGEQHY
                     MYQNLKARKLYMEAMSYTWALNDNEYNFTWRPVVDKSTKLLEASAELEPNASYTIMAL
                     GIQYTFLYEYEKSNKAFEKFLELRPYDLYAKYSLGLVYSKMKQYDKAQKIFEELATKY
                     PEYSDFRIQLADILMNTDKDVQFMKLTDKMMDEPNEKLIGNFLKGIFYSRKDNMDSAI
                     YFYNESKKYFNGYCSICDNNIGHIYFVNNNTDSARKYFRQVLAKDSTYPFAHFNLGVI
                     EDKEGDPSAAIKSFYSTIVNAKGSTEGFITKLHLYLGKSYDTSNRKALEEFSKRTYIF
                     NMAYFSYLSILYAYIRVPGLIDSTTAVDYVFEQLFTYKQYEATTWFHHACYKAMKKDK
                     TGAVESLDKALKLGFGSYYQLIGDKDLDFIRQTPEFNPLLEKYFPEETKKTK"
     assembly_gap    2348..2376
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2377..2948)
                     /locus_tag="PK977_15480"
     CDS             complement(<2377..2948)
                     /locus_tag="PK977_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF19576.1"
                     /translation="MKIKSIFFVLATIIGFAVHAQRGIELPGGDTTKPTGTTYAIIIG
                     LSKYKEVPSLQFAHKDAQAFEDFLLSEAGGKVPRSNIETFLNENATRNNVADAISIMA
                     RKAKPGDRLYFFFAGHGDMEDLTQIENGLLLLYNSPNGNYFGMKDDVLEILDLKRYLS
                     PLAERGVEMIFIVDACHSGNLNGGIKGVEKT"
     gene            3129..>3980
                     /locus_tag="PK977_15485"
     CDS             3129..>3980
                     /locus_tag="PK977_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19577.1"
                     /translation="MGKRILFILFVFVFSITVSSGQNEKKLDSLISLADSLSRNKNFT
                     AALEVNLSAVDLLKKLKENSYFQAMSPVVMLNTGTCYKELGDVKNAHRFMTYSLQLAR
                     SNKVHLDIELAFISLNSLHKHISISNLSFDYPVVPATEELGMFYVISKIEKVSADSVR
                     IIIQGGKYDGIDDSVKKGGIISRYDVNKKNERPFGLVNCYIREINNNYCVAYARDDSV
                     LYIKEGDLVELKTRVPIFWRKLDIGKALLRAISYSTYYREQIFHPRYLYYYADSLTNR
                     DLAAIFKN"
     assembly_gap    3981..4144
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4145..>4437
                     /locus_tag="PK977_15490"
     CDS             <4145..>4437
                     /locus_tag="PK977_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19578.1"
                     /translation="AYPRTYLGSAPKFAEFYATWVINNTPLAPEDVMPWLVNIRDRKA
                     RKNMAANLATTIRENDLIEKWFNDGMLMANSDNIDSSLYMAELLQDVTVALAD"
BASE COUNT         1240 a          835 c          854 g         1315 t
ORIGIN      
        1 caccggaggc cgatccactg cgcaatactt atatacaaca taccaagctg aagaaaatga
       61 tcaataaaat tccggcaaag caggtattgg tactgcttga tatctgtcat ggtggtgtgt
      121 ttgatgagaa tgtactgggc aaaacaagag ataaccctgc agccgccatt accaatagga
      181 atgtgctgga actgctgaaa gataaatccc agtatgtaac ccgtaaagta ttgagctctg
      241 ttgggaaaga gtctgccttt gatggccggg ccggaaaaca ttcccctttt gcagcttact
      301 tgcttaagat attgaatgca agagggggtg ccgagggaat cgttacattg tcagatatct
      361 atgccttgct ccaaaaagca agccttaacg aaacagaaac attgaaaatt tcaccacacc
      421 tcgctggttt tggaaagaat gacccgcaaa gtgaatttat actgataccg gtggaaaaga
      481 aatgataatt actttgtctt ttttgtttcc tccggaaaat atttttccaa taacggattg
      541 aattccggcg tttgcctgat aaaatcaagg tctttgtctc ctattaattg ataatagctt
      601 ccaaatccaa gcttaagtgc cttatcaaga ctttcgacag cacccgtttt atcttttttc
      661 atcgccttat aacaggcatg gtgaaaccag gtggtggcct catattgctt ataggtgaag
      721 agttgctcaa acacataatc aaccgctgta gtactgtcta tcaatccggg cacacggatg
      781 tatgcatata aaattgacag ataggagaaa taagccatgt taaagatata tgtccgcttg
      841 ctgaactctt caagggcctt acggttactg gtatcgtaag attttccgag atacagatga
      901 agtttggtaa tgaaaccttc tgtagaacct tttgcattta caatggtgga ataaaaactt
      961 ttaatagcag cggaagggtc tccttcttta tcttcaataa cccccaggtt gaaatgagca
     1021 aatggatagg tgctgtcttt tgccaaaacc tgtctgaaat actttcttgc tgagtctgtg
     1081 ttgttattga caaagtaaat gtgcccgata ttgttatcac atattgaaca atacccgttg
     1141 aagtatttct ttgattcgtt ataaaaataa atggcgctat ccatgttatc cttgcgggaa
     1201 tagaaaatac ctttcaaaaa attccctatt aatttctcat tcggttcatc catcattttg
     1261 tcagttagct tcatgaactg tacatcttta tcggtgttca tcaaaatatc tgcgagttga
     1321 atccggaaat cagaatattc aggatatttc gttgccaact cctcaaatat tttttgggct
     1381 ttatcatact gtttcatttt ggaataaaca agccccagtg agtattttgc ataaagatca
     1441 tacggcctta attcgaggaa tttttcaaat gctttattag acttttcata ttcatataaa
     1501 aaagtatatt gaatacccag tgccattatc gtataagagg cgttgggctc aagttcggct
     1561 gaagcctcca gaagcttcgt tgatttatct acaacaggcc gccaggtgaa attatattcg
     1621 ttatcattta atgcccaggt ataggacatc gcttccatat atagttttct tgcctttaaa
     1681 ttctgataca tataatgctg ctcacccagt aattgcatac aactgtctaa ttccatggaa
     1741 gcataataac acatgtcacg ggtagaaaag ctggtttcgc ctctcagcag gggagaaaca
     1801 atggagtcga accgctggtt gagtgcggcg gccaggttgc ggcgcattag gggtgtcagc
     1861 ggattatcat tgtatttttt tgcaaaggct ttataatcct tcaaagcatt ggtatcaagc
     1921 ggccagatca gttttctgtc actcaaattc tttttataag aagcataaag ttttttacct
     1981 gcaggatcca gcgaatcaac atatttttct tctacacctt ttgggtttac ctgtgccagt
     2041 gcaggacgtt cttttgcttt ttgaatctta agtgctgcca gtacagcagg attaacattc
     2101 acaaacgatt tacttagatc accaacaatg agtggtattt gtttgtcttc actaaacgtt
     2161 tcaaccttat ctcttatgta gcgatcaagt tctgaaaaac tgatacggcc atcattattc
     2221 cggtcagcaa ggcctttcat accttcttcg agatggagtg aaaacaaacc ccgacctccg
     2281 ccccattctt cactttctaa tgaaagctgg ttgggctggc aggagagaat tttatattct
     2341 ttgccccnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttt tttctactcc ttttataccg
     2401 ccattcaggt tgcccgaatg acaggcgtct actatgaaga tcatttctac acctctttct
     2461 gccaacgggg ataaatatct tttcagatcc aatatttcca gcacatcgtc tttcatgccg
     2521 aaataattgc cattggggct attgtatagc aatagcagac cattttctat ttgggtaaga
     2581 tcttccatat ctccatgacc agcgaagaag aaataaagcc tgtcacccgg ttttgcctta
     2641 cgggccatta tggagatggc atcagccaca ttattacggg tggcattttc attcagaaat
     2701 gtttctatgt tggagcgggg cacttttccg cctgcttcac tcagcagaaa gtcttcaaat
     2761 gcctgggcat ctttatgggc aaattgaaga gagggaacct ctttgtactt gctgagtcca
     2821 atgatgatag cgtaagtagt gccggtgggc tttgttgtat ctcctccggg aagttcaata
     2881 ccccgttggg catgaacagc aaagccgatg atggtggcta aaacgaaaaa aatactttta
     2941 attttcatat cgaatatgtc acattgaagt tacaaaataa ccattaaccc aacttgccgt
     3001 aatccgtgct ggcaggcatt taacggttga ctgttccttt aggaaaccgg ttggcagttt
     3061 gttatgaata ccttccggag ggtattgcta accacttaag acttcgtaaa tttcatattt
     3121 aaaggagtat gggaaaaaga atccttttta tactttttgt atttgttttt tccatcacag
     3181 tttcttccgg gcaaaacgag aaaaaacttg atagcctgat cagtctggcc gattctctga
     3241 gccggaataa gaattttact gctgcactgg aagtaaatct ttcagctgta gacctgttaa
     3301 agaaattaaa ggaaaatagc tatttccagg ccatgtcgcc ggttgttatg ttgaataccg
     3361 gtacatgtta caaagaattg ggggatgtaa agaatgccca ccgcttcatg acttattcct
     3421 tacaattggc ccggtccaat aaagtacacc ttgatattga acttgccttt atttcattaa
     3481 actcactaca taagcatata agtatctcta atctttcatt cgattacccg gtagtaccgg
     3541 caactgaaga actggggatg ttttatgtga ttagcaaaat agagaaagta tccgctgact
     3601 ctgtacgtat aattatccag ggaggaaaat atgatggcat tgacgattca gtaaaaaaag
     3661 gtggcattat ttcccgctat gatgttaata aaaagaatga aagaccgttt ggattagtga
     3721 attgttatat cagggaaatc aataacaact attgtgttgc ctatgccagg gatgattcag
     3781 ttctttatat caaggaaggt gatttggtgg aactaaaaac aagggtcccg atattttgga
     3841 gaaaactgga tataggcaag gctttgctaa gagcaataag ttatagcact tattaccggg
     3901 aacaaatttt tcacccccgc tatctttatt actatgcaga ttctcttacg aacagggacc
     3961 ttgctgctat tttcaaaaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnntgctta tccaagaaca tatttaggtt cagcacccaa gtttgctgag ttttatgcta
     4201 cctgggtaat aaataatacc cctcttgctc ctgaagatgt aatgccctgg ctggttaata
     4261 tccgtgaccg gaaggcaaga aaaaatatgg ctgcaaatct tgcaaccaca ataagagaaa
     4321 atgaccttat tgaaaagtgg tttaacgatg gaatgctgat ggccaactca gataatattg
     4381 attcatcttt atatatggct gagttattgc aggatgtaac agttgcactt gctgata
//
LOCUS       DAORLS010000250         4424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10788_length_4424_cov_1.937972, whole genome shotgun sequence.
ACCESSION   DAORLS010000250 DAORLS010000000
VERSION     DAORLS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_4424_cov_1.937972"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>858)
                     /locus_tag="PK977_15495"
     CDS             complement(<1..>858)
                     /locus_tag="PK977_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF19579.1"
                     /translation="VGFTDALEVGGKMGKLNVITTGITEKGFNWSDQYNLLSGIIGGF
                     FLALSYFGTDQSQVGRYLTAKSLRESRMGLLMNGIVKVPMQFLILLIGVLVFTFYQFN
                     RAPIFFNDVQVKKIEQSAYRDSFYLVQQQYDSISALKQNAVVAYADARHNNDANLADN
                     SLLTLKELQKNSEGLRNKVKGWLNEKIPGGDGNDTNYIFLRFVVDNLPVGLVGLLIAI
                     IFLASWGSIAAAINSLASCSMVDFHRRFTKKKETGEDEYRLSKWYTLGWGIFCIVVAM
                     FTYNIGNSL"
     assembly_gap    859..890
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<891..1487)
                     /locus_tag="PK977_15500"
     CDS             complement(<891..1487)
                     /locus_tag="PK977_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF19580.1"
                     /translation="MSQLDWIVLVVTLLAIVTYGLYKSRTTHNLDGYFLSNRSLPWGL
                     VLLSIMGTQASAITFLSAPGQAYTDGMRFVQYYFGIPLAMVVICIFFVPIFRGLKVYT
                     AYEFLENRFDGKTRTLTSFLFLLQRGLSTGISVFAPSIILSSLLGWDIIWTNLFMGGL
                     LIIYTMTGGAKAVAYTQQLQLIVVFTGMFLAGYMVVNML"
     gene            complement(1613..1786)
                     /locus_tag="PK977_15505"
     CDS             complement(1613..1786)
                     /locus_tag="PK977_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19581.1"
                     /translation="MSNNKPSVKRGEMAFIFAIILGLVLGILIKKVRIGILFGLVLGI
                     VILMTGWLRTNRK"
     gene            complement(1786..>2008)
                     /locus_tag="PK977_15510"
     CDS             complement(1786..>2008)
                     /locus_tag="PK977_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19582.1"
                     /translation="IYHGSDTSGKFEKLIGMADPGEKSDDGSLLVAKYGKGYFTYTGI
                     VLFRQLPAGLPGAYRLLANLIALNKKKGF"
     assembly_gap    2009..2043
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2044..4280)
                     /locus_tag="PK977_15515"
     CDS             complement(<2044..4280)
                     /locus_tag="PK977_15515"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRF19583.1"
                     /translation="MKKYYFLLLVLILELPVFAQGPQSWTSADMYLALRKLNVLGSVL
                     YVAAHPDDENTRLITYLSKDKLYRTGYLSITRGDGGQNLIGEEQGIELGMIRTQELLS
                     ARRIDGGEQFFTRAYDFGFSKNPEETFTKWDKEKILSDVVWVIRKFQPDVIITRFPTT
                     GEGGHGHHTASAILANEAYEAAANPNRFPEQLKYVSTWQAKRVLWNTFNFGGNNTTRE
                     DQFKIDVGGYNPLLGKSYGEIAAESRSQHKSQGFGVPAARGESYEYFKATKGNQPVND
                     LMEGAELSWKRVKGGEAIEKMINELVASFDLLHPEYSVEGLVKLYKAITNLEDGYWKE
                     QKLREIYSLIAQCSGLFLDITTTEQLAVQTDSIRLNFSFNNRLGTDATLQKIIVDKFD
                     TSFLRVLDKNKNFNFSKTIYVPADKPVTQPYWLANKMEEGYFNVTDQLLIGYPDVESA
                     YSAFIRVRIFGEPFTFMRPVRYKFTDPVRGELYQPLVVVPPVIISPSEDLKIAINEKN
                     DINGSLLLKGMKKGFTGKLAAFEIGSDKELKSFNFSSPVATVATKGQVTDITYTTSGL
                     KTNTVAKLGVKANENELVALDKHEIKYDHIPWINYFHEATVTIRNVELKTYNKKVGYI
                     IGAGDKVPEALEQMGYEVTLLTDKELAKNNLEKFDAIITGVRAYNTNEWLNKYYNKLM
                     KYVNDGGNLIVQYNTSNQIGPVRAKIGPFDFNITRTRVTDENAVVNFLKPEHPVFNFP
                     NKLTG"
BASE COUNT         1255 a         1024 c          775 g         1303 t
ORIGIN      
        1 atcaggctgt tgccaatatt ataagtaaac atggctacca cgatacaaaa aataccccag
       61 cccaatgtat accatttgga taaacgatat tcatcttctc ctgtttcttt cttcttcgtg
      121 aacctccggt gaaaatcgac catagaacaa gaagccaatg aattaatggc ggctgcaata
      181 ctcccccatg atgcaagaaa tatgatagca atcagtaatc caacaaggcc tacaggtaag
      241 ttatccacta caaaccggag aaaaatatag tttgtatcat tcccatcgcc cccgggtatt
      301 ttttcattca gccatccttt tactttattg cgtaatcctt ctgaattctt ctgtaattcc
      361 ttcagtgtaa gcaatgaatt gtcggcaaga tttgcatcat tgttatgcct tgcatcagca
      421 taggcaacaa ccgcattttg ttttaatgcg ctgattgaat catattgttg ctgcaccagg
      481 taaaaagaat cacggtaggc tgattgctca atttttttta cttgtacgtc attaaaaaag
      541 atcggcgccc ggttgaactg ataaaaagta aataccaata cccctatcag cagaatcagg
      601 aattgcatcg gtacttttac aattccgttc ataagcaaac ccatgcgact ttctctcagt
      661 gattttgccg taagataacg gcccacctgg ctctgatctg taccgaaata ggataaggcc
      721 aggaaaaaac cgccaatgat gccgcttaat agattatatt gatcgctcca gttaaaacct
      781 ttttcagtaa ttccggtagt tatgacattc agcttaccca ttttgcctcc cacttccagt
      841 gcatccgtaa aacctactnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caacatattc
      901 accaccatat atcccgcaag aaacattccg gtgaatacga cgatcaattg caactgttgg
      961 gtataggcaa cggcttttgc accgcctgtc atggtgtaaa taatcagcag gccacccatg
     1021 aataaattgg tccagataat atcccagccc aataaagaag aaagaatgat ggaaggagca
     1081 aatacactga taccggttga caatcctctt tgcagcagga aaagaaaaga ggtgagtgtc
     1141 cttgtctttc cgtcaaatcg gttttctaaa aactcatatg ccgtatatac tttaaggcct
     1201 ctgaatatgg gcacaaagaa aatacagatg accaccattg ccagcggtat tccaaaatag
     1261 tattgcacaa accgcatccc gtcagtatag gcctgccccg gggctgataa aaaagtaatg
     1321 gcactggcct gtgttcccat gatgcttagg agaacaagcc cccatggaag actcctgttc
     1381 gagagaaaat acccatcgag attatgagtg gtgcggcttt tatacaaacc ataagtcaca
     1441 atggccagca gagtaactac cagtactatc caatccagct ggctcatgca aaacgtttgg
     1501 tgatgagata aaaaataata atcagcacta aaagaaacaa caacacaaac agataccagc
     1561 ctgtccagct tttgaaaaat ggggccttat cgttgtcctg ctgtgtcata aattatttac
     1621 ggttagttct cagccagcca gtcatcaata tcacaattcc taaaaccagg ccgaacagaa
     1681 tcccgattcg taccttcttt atcaatattc ctaagaccaa tccaagaatg atggcaaaaa
     1741 taaacgccat ctctcccctt tttacactgg gtttattatt actcattaaa aacctttctt
     1801 cttatttaat gcaataaggt tggccagtag ccggtaggct cccggcaaac cggcaggaag
     1861 ctggcggaac aggacaatac ctgtgtatgt aaaataacct ttcccatact ttgctacaag
     1921 caggctgccg tcatcagatt tctccccggg atctgccatc ccgatcagtt tttcaaactt
     1981 acccgaagta tcgctgccat gataaatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnntcaccgg ttaacttatt aggaaaattg aaaaccgggt gctcgggttt caggaaatta
     2101 acaactgcat tttcatctgt tacccttgtc cgggtgatat taaaatcaaa aggaccaatc
     2161 tttgctctta ccgggccaat ctggttactg gtattatact gaacaatgag attaccgcca
     2221 tcgttaacat acttcatcaa tttattatag tacttattaa gccattcatt ggtattatat
     2281 gcccttacac cggtaataat tgcatcaaat ttttcaagat tgtttttggc aagctcttta
     2341 tcagttagca aggtcacctc atatcccatt tgttctaatg cctccggtac cttatctccc
     2401 gccccgataa tgtagcctac ctttttgtta tatgttttaa gctcaacatt cctgatcgtt
     2461 accgttgcct cgtggaaata attgatccag gggatatgat catacttgat ttcatgttta
     2521 tcaagagcaa ccaactcatt ctcattagcc ttcacaccta atttcgcaac agtgtttgtt
     2581 tttaaaccag atgtagtata agtaatatca gtcacctggc cttttgtagc cacggtagca
     2641 accggagaac taaaattgaa actttttagc tccttatccg aacctatctc aaatgcagca
     2701 agctttccgg taaatccttt tttcattcct ttcagtaata agctgccgtt aatatcattt
     2761 ttctcattga tcgctatttt taaatcttct gaagggctta ttataacggg aggaaccacc
     2821 accaatggct ggtaaagctc tcctcttaca gggtcggtaa acttatagcg tactggtcgc
     2881 ataaaagtaa aaggctctcc gaatatcctg acccgtataa atgcgctgta ggcagattcc
     2941 acatcaggat aaccgatcaa tagctgatcc gttacattaa aataaccttc ttccatttta
     3001 ttggccagcc aatagggctg cgtaacaggc ttatctgccg gaacataaat ggtcttagaa
     3061 aaattgaagt tcttgttttt atccagtacc cgcaggaagg aagtatcaaa cttatctact
     3121 attatttttt gtaatgtagc atctgtgccc aggcggttgt taaaggaaaa attcagccgg
     3181 atcgaatccg tttgaaccgc taattgttca gtagtagtta tatccaaaaa caaaccgctg
     3241 cactgcgcaa tcagtgaata tatctctctt aatttttgct ccttccagta gccgtcttcc
     3301 aggtttgtga tggctttata caactttacc agtccttcta ctgaatattc agggtgtagc
     3361 aagtcaaaag aggcaaccag ttcattgatc attttttcaa ttgcttctcc cccttttacc
     3421 cttttccagg aaagctctgc tccttccata agatcattga caggctgatt ccctttagtc
     3481 gctttaaaat attcatacga ctctcccctt gcagccggca caccaaagcc ctggctttta
     3541 tgctggcttc tgctttcagc agctatttcg ccatagcttt tgcccagcag gggattatag
     3601 ccacccacat caatcttgaa ctggtcctcc cttgttgtat tattacctcc gaaattgaac
     3661 gtattccaca gtactctttt agcttgccag gttgaaacat attttagttg ctcaggaaaa
     3721 cgattagggt tggcagcagc ttcatatgct tcatttgcca atatagcaga agcggtatga
     3781 tgcccatgtc ctccttcccc ggttgtagga aaacgagtaa tgattacatc cggctgaaac
     3841 tttcggatca cccataccac atcactcaat attttttctt tgtcccattt ggtaaaagtt
     3901 tcttctgggt ttttggaaaa tccgaaatca taagcccttg taaagaattg ttctccgcca
     3961 tctattctgc gggctgatag tagttcttgt gttcttatca tgcccagttc aataccctgc
     4021 tcttctccaa taaggttttg cccaccatcg cctcttgtta ttgagagata gccagtccgg
     4081 taaagcttat cttttgataa ataagtgatc agccttgtgt tctcatcgtc cgggtgagca
     4141 gccacatata aaactgaacc cagtacattc agctttcgta gggccagata catatcagca
     4201 gaagtccagc tttgcggccc ttgcgcaaac acaggtaatt caagtatcaa aaccaataac
     4261 aggaaataat atttcttcat ccggtgaaat tattcggcga atataaatga actaatagtt
     4321 gcgaatctgc cgtccgtaac aataaacccg gtttaaaccg ggtttaacac tcattagtgc
     4381 ttttcttttt tctttataaa ctcgtatccc cctttttctg ctcc
//
LOCUS       DAORLS010000251         4414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10825_length_4414_cov_1.706584, whole genome shotgun sequence.
ACCESSION   DAORLS010000251 DAORLS010000000
VERSION     DAORLS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10825_length_4414_cov_1.706584"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PK977_15520"
     CDS             complement(<1..279)
                     /locus_tag="PK977_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19584.1"
                     /translation="MIETYFNQFRDFVAQPWPWYVAGPLLALVMFILLYFGHEFGISE
                     NFRLMCAADGADNMNEFFKIDWRSGEWNLLVALGAVFGGYLASHYFLGP"
     assembly_gap    336..345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(479..>706)
                     /locus_tag="PK977_15525"
     CDS             complement(479..>706)
                     /locus_tag="PK977_15525"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19585.1"
                     /translation="FLLAVFAGVLGIGFFNFLISFSLAFFMAVKSRGIHLSQYPRLFR
                     TIGHYFFSHPREFFLPPKKVIDLVYKPLREE"
     assembly_gap    707..790
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<791..>2347)
                     /locus_tag="PK977_15530"
     CDS             complement(<791..>2347)
                     /locus_tag="PK977_15530"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19586.1"
                     /translation="RKEPLVLANLQNSLLSQLRNSHLTSAITESGIPLARGFWQELSN
                     RLKHKLLPPLKSDTDFLYVINSVFFRKDDFVWVEAIPRQHWINFFEAIGFSFSPRDTG
                     IKKELLHSLKILSFQVAQLGLERDVLNYIPEEERVHDTAYVRQNYKVHELESLLMDNA
                     SETEITAVSVQLKNLIEDCYRLIDYIRESHSEKGASLQQTYLLLILSNRLQRMQLITD
                     VLDADNKFDTGRFVDLFRLLVRNEKRKNSIREFLSQGVGYLAYQIAEHKGTKGHKYIT
                     ETRREFYKMIYSAMWGGFIISFTAIIKNLIGRLKLAPFPMGFLYSVNYSAGFILIEET
                     KSTLATKQPAFTASAVASSLDTRKIEGEPDIEGLAATVAKVSRSQIASFFGNLVIVFP
                     LTFLLAWLYDLAFGYKIAEGDAALQMLTDQHPWRSPALLYACFTGFFLFVSGIIAGYW
                     QNKIQYGRIKDRLKNHPVLKVSMSAKRLDKLAQYVEKHFGALVGNLALGFFLGFSGVL
                     GKIFGVPFDI"
     assembly_gap    2348..2451
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2740..2789
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2790..>3704)
                     /gene="hutI"
                     /locus_tag="PK977_15535"
     CDS             complement(<2790..>3704)
                     /gene="hutI"
                     /locus_tag="PK977_15535"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRF19587.1"
                     /translation="INLGPVDEVADNIIDASGQMVLPCWCDSHTHLVFAASREEEFVD
                     KIRGLSYAEIAARGGGILNSARKLNAMSESELLRLAWARLNEVMQMGTGAIEIKSGYG
                     LSVEGELKMLRVIKRLKETSPIPVKATFLGAHTYPPEFKENHQGYIELLIKEMLPVIA
                     EEELADYIDVFCETGFFSPGETEQICRAGMSYGLKPKIHANQLNLSGGTQTGVKLGAV
                     SVDHLETMDEDAIQQLAASQTIGTLLPTAAFFLRMPFQPARQLIDGGCAIALATDFNP
                     GSSPSGNMNLVVSMSCIQMKMLPEEAINA"
     assembly_gap    3705..3754
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4106..4172
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4173..4399)
                     /locus_tag="PK977_15540"
     CDS             complement(<4173..4399)
                     /locus_tag="PK977_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19588.1"
                     /translation="MPVLNTITHTITSSNMSVTFDDNTQRTWQVARQRVFTYNNGVVI
                     TVTGMHAANGITGIAEWGTNRFGNFFTTSIA"
BASE COUNT         1217 a          937 c          776 g         1119 t
ORIGIN      
        1 tggcccgagg aaataatgag aggcgaggta accgccaaac acggcaccta atgcaacaag
       61 caggttccac tcacctgaac gccagtctat cttaaaaaat tcattcatgt tgtccgcacc
      121 atccgctgca cacattaaac gaaagttttc agatattcca aattcatggc caaagtataa
      181 taatataaac atcacaagag caagtaaagg gccagcaaca taccatggcc atggctgagc
      241 aacgaaatcc cggaattggt tgaaatatgt ttctatcata actggtagat ttaattacgg
      301 acgtaaagat agaatgaagt gaaaccggct atgctnnnnn nnnnngacaa tcagcataca
      361 ttagactgat attgataagc ttattaacca aaaaaccggt gaattcctgc gcaaaaacaa
      421 cctcttttgt gattgatgtc acactacata attacgaata tgctgataac ccatataatt
      481 attcttcccg taaaggttta tacacaagat ctataacctt tttaggaggc agaaaaaact
      541 ccctgggatg gctgaagaaa taatggccaa ttgttctgaa taatctgggg tattgtgata
      601 aatgtatacc tcttgacttt acagccatga aaaatgccaa agaaaaactg atcagaaaat
      661 taaaaaatcc aatgcccaaa actcctgcaa aaactgccag caaaaannnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn ggatatcgaa tgggacacca aatattttcc ccaatactcc ggaaaagccg
      841 agaaaaaaac caagggccag gttgcccacc agcgcaccaa aatgtttctc tacatattga
      901 gccagtttat caagtcgttt ggccgacata gagactttaa gtacagggtg atttttcaat
      961 cggtctttta ttcgtccata ctgaatcttg ttttgccaat accctgcaat aataccactg
     1021 acaaataaaa aaaagccggt aaaacaggca tacaataatg caggactacg ccacggatgc
     1081 tgatcagtga gcatttgcaa cgctgcatca ccctctgcaa tcttgtaccc aaaagccaaa
     1141 tcatacaacc aggccagcaa aaatgtaagc gggaaaacaa tcaccaggtt accaaagaag
     1201 gaagctatct ggcttcgtga aacttttgca accgtggcag ccagcccctc tatatccggc
     1261 tccccttcaa tcttcctagt atccagtgaa ctcgccactg cactggcagt aaacgccggt
     1321 tgttttgttg ccagggtgga tttagtctct tcaatcagaa taaagcctgc actgtaattg
     1381 acactgtaaa gaaaccccat gggaaaaggc gccagtttta aacgacctat cagatttttg
     1441 ataatcgcag taaatgaaat gataaaaccg ccccacatgg cgctatagat cattttgtaa
     1501 aactctcttc tcgtctcagt aatatattta tgtcctttgg ttcccttatg ttcagcaatc
     1561 tggtaagcca ggtatccaac accctgcgat aaaaactccc tgatactgtt ctttcttttc
     1621 tcgtttcgta ccaacaacct gaaaagatca acaaaccggc cggtatcaaa cttattgtct
     1681 gcatccagta catctgttat cagctgcatt cgctgaagac ggtttgacaa aatcagtaac
     1741 aggtacgtct gctggaggct ggcccctttt tcggaatggc tttccctgat ataatcaata
     1801 agcctgtagc aatcctcgat caggttcttt aattgaactg aaacagccgt aatctccgtt
     1861 tcagaagcat tatccatcaa caggctttcc agttcatgga ccttgtaatt ctgtcgcaca
     1921 taagcagtat catgcactct ttcttcttca gggatataat taagaacatc tctttccagt
     1981 ccaagctgtg ccacctggaa agaaagaatc tttagtgaat gaagcaactc tttttttatc
     2041 ccggtatctc tgggactgaa gctaaaccca attgcttcga aaaaatttat ccagtgctga
     2101 cggggtatag cttccaccca tacaaaatca tctttccgga agaaaacgct attgatgaca
     2161 tatagaaaat cagtatcact tttaagcggg ggtaatagtt tatgttttag tctgttggat
     2221 aattcctgcc aaaagcccct ggctaatgga atgccgcttt ctgtaatagc agatgtaaga
     2281 tgactgttcc tgagttgtga caatagggaa ttttgcaggt tggccagtac taatggttct
     2341 tttcttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcatcaac
     2461 aggaaggata gtgttttctt ctactacctg ttcaaagaaa ccaggtttct tttttcttaa
     2521 ccggatcatg cagaccgaaa atacaacttg tagacttttg agaagtatta aaaaattata
     2581 agaaaattaa aagaatatta taaccattcc ccttcaatca tcaccctgtc tatcgggttt
     2641 gtcccaaagg aatagggcaa ataggccagg gatggaacgg gccttgtgaa tatcaggttt
     2701 gcctttttgc caatattgat gctaccaact tcatcctgcn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cattaatggc ttcttccggc agcattttca
     2821 tttgaataca actcatggaa accaccaggt tcatattacc tgaaggagaa gaaccagggt
     2881 taaaatcagt tgccagtgca atagcacagc cgccatcaat taactgtctt gccggttgaa
     2941 aaggcatacg aagaaaaaaa gcggctgtgg gtaataatgt cccgatcgtt tgcgatgcag
     3001 ccagttgttg tattgcatct tcatccatag tttccaggtg atcgacactc acagcaccca
     3061 gcttcactcc tgtttgcgta ccaccggaca aatttagttg gttggcatgt atcttcggtt
     3121 ttaacccata gctcatacca gcccggcata tttgttctgt ttccccgggt gaaaaaaatc
     3181 ctgtctcaca aaacacatca atataatcag ccagttcttc ttcagcaata acaggcagca
     3241 tttcttttat cagcaattca atatacccct ggtgattttc tttaaattcc ggagggtacg
     3301 tatgtgcccc caaaaacgtt gctttcaccg ggataggtga ggtttccttc aatcgtttga
     3361 tcacacgaag catcttcaat tcgccttcca ccgataaccc ataaccgctt ttgatctcga
     3421 tggcgcctgt ccccatttgc attacttcat tgagccttgc ccatgccagg cgcaatagtt
     3481 ctgattcact catcgcattc agtttccggg ctgaattcag tatgcctcct ccccttgcag
     3541 caatctcggc ataactgagt ccccttatct tatcaacaaa ctcctcctcc cggctggcag
     3601 caaaaacaag atgagtatga ctgtcgcacc agcagggtaa aaccatttgc ccggatgcat
     3661 caattatatt atctgccacc tcgtcaacag gccccaggtt aatcnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaaaaa ggcgttctct acacatggaa
     3781 gtaccgaaag ctcttttccc ctgagtaatt tattctcaaa tcgggtattg acaagcaatt
     3841 taatattggt gaccagtgtg gaagccatac agccgctaat ttacctgtaa aagctgattt
     3901 gtttacaata aaaaaagccg ccctcgcagg cggctcagtt aactaaaaaa gtttattaat
     3961 aaggcaataa agtagattgg gtattaccct gcgggcccgt ccattccacc ttcatatagt
     4021 agggattggc gccggggcat cctgtcggat taccattagc attaagcccg aaagttaccg
     4081 atgcattgaa aagattggta tgatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgtgcaat agacgtggta aaaaaattgc
     4201 caaaccgatt ggttccccat tcagcaatac ccgtaatgcc gttggcagca tgcattccgg
     4261 ttactgttat cactactccg ttattgtaag tgaatactct ttgacgggcc acctgccagg
     4321 tacgctgtgt gttgtcatca aatgttacag acatattgct gctggtaatg gtatgggtaa
     4381 tcgtattcag aactggcaaa ttgataagta atcc
//
LOCUS       DAORLS010000252         4408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10842_length_4408_cov_1.914542, whole genome shotgun sequence.
ACCESSION   DAORLS010000252 DAORLS010000000
VERSION     DAORLS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10842_length_4408_cov_1.914542"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..119
                     /locus_tag="PK977_15545"
     CDS             3..119
                     /locus_tag="PK977_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19589.1"
                     /translation="MKLFNEIESEIKGKVVKILVDDASPVEYDQPLFLVEPA"
     gene            152..>742
                     /locus_tag="PK977_15550"
     CDS             152..>742
                     /locus_tag="PK977_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRF19590.1"
                     /translation="MFKKILIANRGEIALRVIRTCREMGIKTVAVYSTADRDSLHVKF
                     ADEAVCIGRPQSADSYLNIPHIMAAVEITNADAVHPGYGFLAENAKFAQICADHDIKF
                     IGPTPDMINAMGDKITAKETMIKAGVPVVPGGEGLLQSVDEAKGLAKEMGYPVILKAT
                     AGGGGKGMRIVWEESELERAYDTAKAEAAAAFKNDGI"
     assembly_gap    743..836
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <837..1479
                     /locus_tag="PK977_15555"
     CDS             <837..1479
                     /locus_tag="PK977_15555"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRF19591.1"
                     /translation="RRHQKLVEESPSPFMTDELRHAMGEAAKKAAKAINYESVGTIEF
                     LVDKHRNFYFMEMNTRIQVEHCVTEEVINFDLIKEQILIAAGHKISGKDYYPEMHSIE
                     CRINAEDPYNDFRPSPGKITVLHQPGGHGVRVDSHVYAGYVIPPYYDSMIGKLITVAR
                     TRQEAIDTMYRALSEYVIEGVKTTIPFHLQLMKDERFRSGNFNTKFLDGFTLK"
     gene            1550..2728
                     /locus_tag="PK977_15560"
     CDS             1550..2728
                     /locus_tag="PK977_15560"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRF19592.1"
                     /translation="MKYLLFLIVCFSIISCSTALNKLHQKAVLADTHNDVLSSQVLEG
                     KDISHRMKEGHSDLDRWKEGGMDVQFFSVWTDKTPRNKAGFFADAVEEIDSLHHIILR
                     NPVRMAFATNYKEVKKWVHKDRLVSLIGVEGGHMIEGEISKLERLYEKGMRYLTLTWN
                     NSTDWASSAMDETLNPGKLKQKGLNDFGKQVVRRMNDLGIIVDLSHVGEQTFYDALAV
                     TTKPVFLSHSSVWSICPVFRNVKDEQIKAVAQNGGVICINFYSGFISKEYDQRQSYLN
                     KVARDSAFRSFLAATNGDSVAAKAKWEQYYDAEMNKVRPSLAQLVDHIDHIVKLVGDD
                     YVGIGSDFDGVSSLPVGLDDVTTYPKITEELLKRGYSRKSVKKILGGNVLRVMKANFK
                     "
     gene            complement(2774..3058)
                     /locus_tag="PK977_15565"
     CDS             complement(2774..3058)
                     /locus_tag="PK977_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19593.1"
                     /translation="MKKSIGTFFIILSCGMLIACGGDKKEKTAAEIAAEWCELNGKVA
                     KAAEGAEKEAAREAIKKWENEIEAKYKDNEAFIKEIQNEAEKCEGASEGR"
     gene            3176..3571
                     /locus_tag="PK977_15570"
     CDS             3176..3571
                     /locus_tag="PK977_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19594.1"
                     /translation="MLKIGQLKSGDVISINDEGIMREGTVVDISHEEHQALVNNGVQE
                     FWFSQEEMFAVPLDESHLLKFGFTGEEAEDGYKYKKDSFRVLVPKKGDFSKMEMWWRE
                     DRRHFYTPLSVHEFQNLYMDMTKIHLDMP"
     gene            3683..3901
                     /gene="infA"
                     /locus_tag="PK977_15575"
     CDS             3683..3901
                     /gene="infA"
                     /locus_tag="PK977_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRF19595.1"
                     /translation="MSKQPLIKQDGTIIEALSNAMFRVKLENGHEILATISGKMRMHY
                     IRILPGDKVGVELSPYDLTRGRINFRYK"
     gene            3945..4061
                     /gene="ykgO"
                     /locus_tag="PK977_15580"
     CDS             3945..4061
                     /gene="ykgO"
                     /locus_tag="PK977_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRF19596.1"
                     /translation="MKVRASIKKRSADCKIVRRKGKLYIINKKNPRFKQRQG"
     gene            4101..>4408
                     /gene="rpsM"
                     /locus_tag="PK977_15585"
     CDS             4101..>4408
                     /gene="rpsM"
                     /locus_tag="PK977_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRF19597.1"
                     /translation="MARIAGVDLPKNKRGEIGLTYIYGIGPSTAKYILDKNNIDRSKK
                     VHEWNDDELNAIRNTITEELKVEGQLRSEVQMSIKRLLDIACYRGLRHRKGLPVRGQR
                     T"
BASE COUNT         1278 a          805 c          998 g         1233 t
ORIGIN      
        1 cgatgaagct tttcaatgag attgaaagtg aaatcaaagg caaggtggtg aagatactgg
       61 tggacgatgc atcaccggtt gaatatgatc agccgctgtt tcttgttgag ccagcctaat
      121 tagcatatta tccaattatc aaattcactc catgttcaag aaaatattga ttgctaacag
      181 gggagagatt gcccttcgtg ttataagaac ctgccgtgaa atgggtatta aaacggtagc
      241 cgtttattct actgcggacc gtgacagcct ccatgtaaaa tttgctgatg aggcagtttg
      301 tattggccgt ccccagagtg cagattccta tttgaatatt cctcatatta tggcagcggt
      361 ggaaatcacc aatgctgatg ccgttcaccc gggctatggc ttcctggcag aaaatgccaa
      421 gtttgcccag atttgtgccg atcatgatat taagtttatt ggtccaacac ccgatatgat
      481 caacgccatg ggtgataaga tcaccgctaa agaaacgatg attaaggcgg gggtgccggt
      541 agtacccggt ggtgaaggtt tattgcagag tgtagacgag gctaaaggtt tggcaaaaga
      601 aatgggttac cctgtaattt taaaggctac cgccggtggt ggtggaaagg gtatgcgtat
      661 cgtttgggaa gaaagtgaac tggagagagc ttatgatact gctaaagccg aagcggcagc
      721 cgctttcaag aatgacggta ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaga
      841 cgccaccaga aactggtaga agaatctcct tctccattta tgacagatga actgcgtcat
      901 gcaatgggtg aggccgctaa aaaagcagct aaagccatca attatgaaag tgttggaact
      961 atagagttcc ttgtagacaa gcatcgcaat ttctatttca tggaaatgaa tacccgtatc
     1021 caggtagagc attgtgttac ggaagaggtt atcaactttg acctgatcaa agaacagatt
     1081 ctgattgcag caggccacaa gatatccgga aaggattatt acccggaaat gcattcaatt
     1141 gaatgtcgta taaatgctga ggatccttat aacgattttc gtccatcacc gggaaagata
     1201 acagtccttc atcagcccgg aggccatgga gtgcgggttg acagccatgt ttatgccggg
     1261 tatgttattc ctccctatta cgattccatg ataggaaagt taataacggt agcccgtaca
     1321 agacaggaag ccattgatac catgtacagg gcattgagtg agtatgtaat tgaaggagtg
     1381 aagacaacaa taccttttca tcttcagtta atgaaggacg aacgtttccg gagtggtaat
     1441 ttcaatacta agtttttgga tgggtttacc ctgaaataaa aatcattctc aacttttaat
     1501 cctccgtaac agcgggggat tttttaattt catattgtat ttgccctgta tgaaatattt
     1561 acttttttta atagtttgtt ttagtatcat cagttgttca actgcattga ataaactgca
     1621 tcaaaaggcg gtgctggcgg atacgcataa tgatgtgctt tcatcacagg ttttggaggg
     1681 caaagacatt tctcaccgta tgaaagaagg tcatagtgat cttgaccggt ggaaagaagg
     1741 tggtatggat gttcagtttt tttctgtatg gactgacaaa acaccccgta ataaggcagg
     1801 cttttttgct gatgcggtgg aagaaattga ttcgctccat catatcatat tgcgtaaccc
     1861 tgtcagaatg gcttttgcca ccaactataa ggaagtaaaa aagtgggttc acaaagacag
     1921 gctcgtttca cttattgggg tggaaggcgg acacatgata gaaggcgaga ttagtaaact
     1981 cgaaaggtta tacgaaaagg gtatgcgtta cctcactctt acctggaaca acagtaccga
     2041 ctgggccagc agcgcaatgg atgaaaccct taatcccggt aagctaaagc aaaaaggatt
     2101 gaatgatttt ggaaagcagg tggttcgccg gatgaatgat ttaggaatta tcgttgacct
     2161 ttctcatgtt ggtgagcaaa ctttttacga tgcgttggct gtgacgacaa agcccgtttt
     2221 tttatcccat agttctgtgt ggagtatttg tcctgttttc agaaatgtga aagatgaaca
     2281 gataaaagcg gttgctcaaa atggaggtgt aatttgtatt aacttttatt ccggctttat
     2341 cagcaaagaa tacgatcagc gccaaagcta tttgaacaaa gttgccaggg attctgcttt
     2401 tcgttctttt ttagcagcaa ctaatggcga ttcagtggcc gctaaggcaa agtgggaaca
     2461 gtattatgat gcagagatga ataaagtgag gccttcgctt gcccaactgg tcgatcatat
     2521 tgatcatatt gtaaaactgg tgggtgacga ttatgttgga atcgggtcag actttgatgg
     2581 tgtcagttct ctgcctgtag ggcttgatga cgtaaccact tatccaaaga ttacggaaga
     2641 gttgttaaag cggggttaca gcagaaaatc ggttaaaaaa atattaggag gaaacgtact
     2701 ccgtgtgatg aaagcgaact ttaaataaaa aacctgccgt gtcagcaggt tttctcgtgg
     2761 actgaagctc tgattatctt ccttccgaag caccctcaca tttttcagcc tcgttttgta
     2821 tctctttaat aaaagcttcg ttgtctttgt attttgcctc tatttcattt tcccattttt
     2881 taatagcctc cctggcagcc tctttttctg caccttctgc agctttggct acttttccat
     2941 tcaattcaca ccattctgca gcaatttctg ccgctgtttt ttcttttttg tctccgccgc
     3001 atgcaattag cattccgcag cttaaaatga tgaagaatgt gccgatactt tttttcatgt
     3061 tgttgatttt ggttaatgat aagtcttaaa gctacaacat catttaaaaa acaacgcagg
     3121 tcatttgagg atatatcgaa tcttttttat attcggataa ctaaaacgaa ttgctatgct
     3181 taaaatcgga caactcaaat caggcgatgt aatcagcatc aatgatgaag gaattatgag
     3241 ggaaggcaca gtagtggaca taagccatga ggagcaccag gcattggtta ataacggggt
     3301 gcaggaattc tggttcagcc aggaagaaat gtttgctgtt ccgctcgatg agtctcattt
     3361 actgaaattt ggttttaccg gggaggaggc agaggatggg tataaataca aaaaggactc
     3421 attccgggta ctggtgccaa aaaaagggga tttttcgaaa atggagatgt ggtggaggga
     3481 agacagaagg catttttata cccctttatc ggttcatgag ttccaaaacc tgtacatgga
     3541 catgaccaaa atacacctgg atatgcccta aaaaggccga tttattgaga atcaatactc
     3601 tttttccaca acccccttaa aaaaattggg ggttttttct tttttccccc tgatacactt
     3661 acctttgcgt tcccaaaaaa atatgtcgaa acaacccctt ataaaacagg acggtacgat
     3721 tatcgaagct ctgtccaacg caatgttcag ggtaaaactg gaaaatggcc acgaaatact
     3781 ggctactatc tccggtaaaa tgaggatgca ctatatccgc atcctgcccg gtgataaagt
     3841 gggagttgag ttgtcgccat atgatttaac taggggaaga atcaatttca ggtataaata
     3901 acccttcgac aagctcaggg taacaacgtt aaaaactgat tattatgaaa gtaagagcat
     3961 caatcaaaaa gcgcagcgcc gactgtaaga ttgtacgtcg caagggcaag ctgtacatca
     4021 ttaacaaaaa gaatccccgc ttcaaacaaa gacagggata attaatctga attttaaaat
     4081 cgtaaactta aaatagtatt atggctcgta ttgccggtgt tgacttacca aaaaataaaa
     4141 gaggcgaaat aggacttacc tatatctatg gtattggtcc ttctactgcc aagtacattc
     4201 ttgacaagaa taatattgat cgtagcaaga aagtacatga gtggaatgat gatgaactga
     4261 atgctatccg taatactatt acggaagagc tgaaggtgga aggtcagctt cgttcagaag
     4321 tgcagatgag cattaagcgt ttgctggata tagcctgcta ccgtggtctt cgtcatcgta
     4381 aaggtctgcc ggttcgtggg cagcgtac
//
LOCUS       DAORLS010000253         4390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10897_length_4390_cov_2.159170, whole genome shotgun sequence.
ACCESSION   DAORLS010000253 DAORLS010000000
VERSION     DAORLS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10897_length_4390_cov_2.159170"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1487
                     /locus_tag="PK977_15590"
     CDS             186..1487
                     /locus_tag="PK977_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HRF19598.1"
                     /translation="MKKVLIFGMILLIAGGQANAQFFQRGTCSTPVGQLMLKEKDSLG
                     SRGVSDNYHLWDNGTTLLVKFMPGGSKILRDKVMLYAREWSKFGNINFKFVADTASFT
                     HVRVKLGKGYGHNSAVGTESKFRRQNEQTMNFDTLYWADADYYVAKLKAKNINPPYNL
                     NQLIAEMRADPNHWNEKELRRVVMHEFGHALGLLHEQSYPGAIKWKKTDSVYNYYWQT
                     QGWDKEKVDFNVFEVSNQFYTNGTTYDPKSIMHYSIAAWQTTDGYTMKDNYELSTGDK
                     TLIGALYPKGKKVSDLEVPRVDVSNFGKMNVMSDANRRGLVITPSFDLKSNSKTGEVY
                     FVARLATEDGYYIQTTSPYYNWGGTAAVYQKINVLPNAKVSYNKAGKKNLELFIPYNQ
                     IPETYGKKVIVVFAVYLDDVKNKQMDKLMYFSTTTPMSLPR"
     assembly_gap    1568..1594
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1632..1705
                     /locus_tag="PK977_15595"
     tRNA            1632..1705
                     /locus_tag="PK977_15595"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1666..1668,aa:Pro,seq:tgg)
     gene            1755..2726
                     /locus_tag="PK977_15600"
     CDS             1755..2726
                     /locus_tag="PK977_15600"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRF19599.1"
                     /translation="MHIVVTGGAGFIGSNLVKKLYAVYQGIKITCIDNFDPFYSATLK
                     QHNISHFMQRQGFRFINTDIAITSSKELLNLIPEPVDVIVHMAAKAGVRPSIENPMAY
                     QQVNVIGLQNMLEFAKSAGVKQFVFASSSSVYGINDHFPWKEDEQLLPISPYAMTKLA
                     GEMMGHVYSKLFGIRFIALRFFTVYGPGQRPDLAIHKFTKAILKQEPITMYGDGTTSR
                     DYTFIDDIVQGVIKAIAYTQSDFEIINLGNNYAVSLNELIHSVEQTVGKKAIIQQLPD
                     QPGDVPQTFADISKAKSLLEYDPKTKLKEGLAKFYDWFRENEKLLLD"
     gene            complement(2727..>3601)
                     /locus_tag="PK977_15605"
     CDS             complement(2727..>3601)
                     /locus_tag="PK977_15605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF19600.1"
                     /translation="YEIILVDDGSTDKTKQQILEHADDRTVLVELRKNYGQSTAMTAG
                     IDHSHGRYIALLDGDLQNDPTDIPAMLELLKKEDWDVVAGNRKNRKDGFILRKIPSKI
                     ANALIRKMTGVYIKDYGCTLKVFRREIAEELGLYGELHRFIPVLAKLQGAKITQVDVK
                     HHPRIHGKRKYGLNRTFKVMSDLVLMVFMRKYMQKPMHLFGPIGFVSFGIGVAINIYL
                     LVLKIMGNDIGGKPLLILGLILLLGGIQFITIGIVADINVRTYFESQNKKTYTVRRIY
                     HAKREIMQHSNAAM"
     assembly_gap    3602..3647
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3779..>4390)
                     /locus_tag="PK977_15610"
     CDS             complement(3779..>4390)
                     /locus_tag="PK977_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRF19601.1"
                     /translation="NILFNGIQNIHAADAVVAKSKGQVIKLVAQAQKLKTGQVTAFVL
                     PQFVRQDDHLSFVKNEYNGVVIESGFADKQFFYGKGAGSFPTASAVLSDISALRYQYK
                     YEYKKLYHHQQHILSDEVFLRVYISFDDISLVPRDKFEWIEEWHAQEERKYLVGVISF
                     TELKENSWWRENNTSLIVAADPVIEDIEIRKLKKKSLQLAGIL"
BASE COUNT         1293 a          851 c          875 g         1298 t
ORIGIN      
        1 acctgtaccg agggttcgaa tccctccctc tccgcatcag ccagccgaag cttttgctct
       61 gactggcttt tttattgaga gagctttttg attgttttaa cagccatgac gtcaatatac
      121 cgtacttcgg gtatgatgat gcaaccaaag caatatattt tcgacttcaa ttaaatcaaa
      181 ctaatatgaa aaaggtcctg atttttggaa tgattctcct gattgccggt ggacaagcca
      241 atgcccagtt ttttcaaagg ggtacctgca gcactcctgt agggcaattg atgctcaaag
      301 aaaaagattc acttggtagt agaggtgtta gtgataatta ccacttatgg gataatggta
      361 ctactctact ggtgaaattc atgcccggtg gcagcaagat attaagagat aaggtaatgc
      421 tatatgccag ggagtggtcg aaatttggta atataaattt caaatttgta gcagatactg
      481 cttcttttac ccatgtaaga gtgaagctgg gtaagggata tggtcataat tccgcagtgg
      541 gaacagagtc taagtttcgc aggcagaatg aacaaacgat gaattttgac actttgtact
      601 gggctgatgc tgattattat gtagcaaaac tgaaggcaaa gaacataaat cccccctata
      661 atcttaacca gcttattgca gagatgaggg cagatcccaa tcactggaat gaaaaggaat
      721 tacgcagggt ggtgatgcat gaatttggcc atgcacttgg attattgcat gagcaaagct
      781 acccgggtgc gatcaaatgg aagaaaactg attctgtata caattattac tggcagacac
      841 agggatggga taaagaaaaa gttgatttta atgtctttga agttagcaac cagttttata
      901 caaacggaac aacgtacgat cccaaatcga tcatgcatta ttcaatagca gcctggcaga
      961 caacagatgg ctatactatg aaggataact atgaactttc aacaggtgat aaaacattga
     1021 ttggcgcttt atatcctaaa ggcaaaaagg tttcagacct ggaagtgccc agagttgatg
     1081 tcagcaattt cggtaagatg aatgtaatga gtgatgctaa ccgaagagga ttggtaataa
     1141 caccttcatt tgatctgaag tccaattcaa aaacaggcga agtatatttt gtagcaagac
     1201 tggcaacaga agacgggtat tatattcaaa ctaccagccc atattataat tgggggggaa
     1261 cggcagccgt ttatcaaaag ataaatgtac ttcccaatgc aaaggtgagt tataataaag
     1321 ctggcaagaa aaacctggag ttatttattc cttacaacca gataccggaa acatatggaa
     1381 agaaagtaat tgtagtattt gccgtttacc tggacgatgt aaaaaataaa cagatggaca
     1441 agctgatgta tttttcaaca acaactccaa tgagtttacc aagatagttt tattaatgtt
     1501 taactgaagc cggtaacagc caacctgttg ccggtttttt atttattaaa tgaaaaaatt
     1561 aagcctgnnn nnnnnnnnnn nnnnnnnnnn nnnncgttat atttgcactc cattttcaaa
     1621 atggaaaggc tcgggaggta gttcagcccg gtagaatacc tggtttggga ccagggggtc
     1681 gcaggttcga atcctgtcct cccgacacta aagaatcctg cgatgagcag gattctttgt
     1741 ttaaaatgac ttcgatgcat attgttgtaa caggcggcgc tggttttatc ggtagtaatc
     1801 tggtaaagaa actttatgct gtatatcaag gtataaaaat cacctgcatc gacaattttg
     1861 atcccttcta ttcagcgacc ctcaagcaac ataatatcag ccacttcatg caaaggcagg
     1921 gcttcaggtt tataaataca gacatagcta ttacttcatc aaaagaactt cttaacctga
     1981 tacctgaacc agttgatgtg attgtgcaca tggctgccaa ggctggtgta aggcccagta
     2041 tagagaaccc gatggcctac cagcaggtaa acgttattgg cttacaaaac atgctggaat
     2101 tcgccaaaag tgcaggtgtc aaacaatttg tatttgcttc cagcagcagt gtgtatggga
     2161 ttaatgatca ttttccatgg aaagaagatg aacagttatt gcccattagc ccctatgcga
     2221 tgaccaaact ggcaggggaa atgatggggc atgtttatag taaattattt ggtatccgtt
     2281 ttattgcgct ccgctttttc accgtttatg gtccgggtca gcggccagac ctggcgattc
     2341 ataagtttac aaaagccata ttaaaacaag aaccaattac aatgtatggt gatggtacta
     2401 ccagcaggga ttatactttt attgatgata ttgttcaggg tgttataaaa gctattgcat
     2461 atactcaaag tgattttgag ataatcaatc ttggaaataa ttatgctgtt tcactaaatg
     2521 aactgatcca ttctgttgaa caaacagttg gtaaaaaagc cataatacag cagttgccgg
     2581 accagccggg cgatgttccc caaacttttg cggatatcag taaagccaaa agtttgctgg
     2641 agtatgatcc caaaacaaaa ctaaaagagg gtcttgcaaa attttatgac tggttcaggg
     2701 aaaatgagaa actgttattg gactaatcac atagctgcat ttgagtgctg cattatttcc
     2761 ctttttgcgt ggtatattct tcttacagta taggtttttt tgttttgcga ctcaaaatac
     2821 gtccttacgt tgatgtctgc tacaatgccg atggtaataa actgaatgcc accaagcaac
     2881 agtattaatc ccaggatcag cagaggtttt ccgccaatat cattccccat tatttttaat
     2941 acaagcaggt aaatattgat ggccacgcca attccaaaac tcacaaagcc aataggtcca
     3001 aacaggtgca tgggtttctg catgtatttc cgcataaata ccatcagcac aaggtcactc
     3061 atcactttga aagttctgtt cagtccatat ttgcgtttac catgaatacg gggatgatgt
     3121 tttacatcca cctgggttat tttggcccct tgtagtttgg ccaaaacagg tataaacctg
     3181 tgtagttcgc catataagcc caattcttct gctatttctc ttctgaaaac ctttaaagta
     3241 caaccataat ctttgatgta aacgcctgtc atttttcgga tcaatgcgtt ggcaatttta
     3301 ctgggtattt tacgaagaat aaaaccgtcc ttcctgtttt tgcgattgcc ggccactaca
     3361 tcccagtctt cctttttcag taactccagc atggcaggaa tatctgtagg atcattttgc
     3421 agatcaccat ctaacagcgc tatgtacctg ccatgtgagt ggtcaatacc agctgtcatg
     3481 gcagtacttt gcccatagtt ctttctcagt tccaccagta cagttctgtc atcagcatgt
     3541 tcaagtattt gctgtttggt tttatctgtt gagccatcat ccactaatat tatttcatag
     3601 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct tcctcattca
     3661 tcaccgtaat aactaccgac aattctttca tattagctca gtaaggagca aaggtaaggc
     3721 ttcacaaaaa aagagggaag cttttgcatt ccctcagaat agttatcacg agattcgctt
     3781 ataatattcc tgccagttgc agacttttct ttttcagttt tcttatttca atatcctcaa
     3841 taaccgggtc agctgccaca ataagtgatg tgttgttctc tctccaccaa ctattttctt
     3901 tcaattcagt aaaagaaata accccgacaa ggtattttct ttcttcctgt gcatgccatt
     3961 cttcaatcca ttcaaactta tcacggggaa ctaacgaaat atcgtcaaag cttatgtata
     4021 ccctgagaaa tacttcatca ctcaagatat gctgctgatg gtgatatagt ttcttgtact
     4081 cgtatttgta ttggtaacga agggctgaaa tgtcgcttaa aacagctgat gctgtaggaa
     4141 aacttccggc tcccttgcca taaaagaatt gtttgtcggc aaagccgctt tcaattacta
     4201 caccattgta ttcatttttt acgaaagaaa ggtgatcatc ctgtcttaca aactggggca
     4261 atacgaatgc tgtgacctgg ccagttttca gtttctgtgc ctgtgctacc agttttatta
     4321 cctggccttt tgatttggcc actacagcat cggcggcatg tatattttga ataccattaa
     4381 acaagatgtt
//
LOCUS       DAORLS010000254         4372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_10954_length_4372_cov_2.045170, whole genome shotgun sequence.
ACCESSION   DAORLS010000254 DAORLS010000000
VERSION     DAORLS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10954_length_4372_cov_2.045170"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK977_15615"
     CDS             complement(<1..442)
                     /locus_tag="PK977_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19602.1"
                     /translation="MQTQQNIEDRLWSFIDGTAEASEKSVIEKLLETDAAWKTKYREL
                     LEVNEMLRSSELEEPSLRFTKNVMEEIARLHIAPATKTYINKKIIWGIGFFFIAMLVG
                     FLIYGFGQMAFAPTGEESEISKTVSKVDFSKFFNNSWVNGLMLVN"
     gene            complement(458..1039)
                     /locus_tag="PK977_15620"
     CDS             complement(458..1039)
                     /locus_tag="PK977_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF19603.1"
                     /translation="MSTGLNDNELISRVLSGDQQAYAGLVSRYQIYVFTLALRMVKSR
                     EDAEEVAQDSFIKAYKYLGDFKGASKFSTWLYTIVNNTAISFLRKKKVEIHSLDNEKV
                     FEVADSKESGMGANMIEQKSKMLMVNEAIAMLNPDDAQIITLFYKAEQSLEETAQILG
                     IEINAAKVRLYRARTRLKEKMETYFAEEVKDLN"
     gene            1302..1655
                     /locus_tag="PK977_15625"
     CDS             1302..1655
                     /locus_tag="PK977_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19604.1"
                     /translation="MQAAEALVPITMFAGGFAMVFGIAYLKTRENMAMIEKGMNPKDK
                     TNRPAPFKSLKAGLLFLGAGLGLLTAFFLDMYVIPHEFEPVGLYFALIAIGGGLGLIG
                     SYSVERKHWYDKANK"
     gene            complement(1713..>2975)
                     /locus_tag="PK977_15630"
     CDS             complement(1713..>2975)
                     /locus_tag="PK977_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF19605.1"
                     /translation="FSDTVNVVYVIPGGPSDKAGLMIGDKILKVNDSSIAGRKMQVDD
                     IKKYIRGPRGSKANLQIIRAGALKMLTVTRGTIPVSSVDATYMLDKTTGYIKLNRFTE
                     NSYEEFMEALENLKKQGLKGLVFDLRGNGGGFMNEAVDIADEFLEGDKLIVYTEGVNS
                     KKVEYRCKRPGQFETGKLVLLVDELSASASEVIAGCLQDWCRATIIGRRTFGKGLVQQ
                     QYDLSDGAAIRLTVARYYTPLGRSIQRSYEKGKKVYMDDLWERFNSGEMVSYDSIKRH
                     NGGKKFLTSCKDTLYAGDGITPTVFIPLDTTLYHRELNGLLTGNSMNNYVYNYYLQHK
                     QHIDQYKSSDEYAKGFNAVTEMWAGWVKAAITDSVNLSTINGRKKEMLQLRLKALLAR
                     FKWRNTGFYQVLNYDDMAVRKAMEVISK"
     assembly_gap    2976..3000
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3001..3325)
                     /locus_tag="PK977_15635"
     CDS             complement(<3001..3325)
                     /locus_tag="PK977_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl-terminal protease"
                     /protein_id="HRF19606.1"
                     /translation="MMGNKKLQVWLPLIFSLVMITGMMLGYKLGSQGGGKSKFFSSNK
                     RSSLQEALDLIRLKYVDSVRIDSLEGKAIQEMMNELDPHSVYLPPVDLKEANEDLAGN
                     FQGIGV"
     gene            3421..4263
                     /locus_tag="PK977_15640"
     CDS             3421..4263
                     /locus_tag="PK977_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HRF19607.1"
                     /translation="MASIKLIADSGATKAEWCLVNGGKVKTIFTQGISPYFLNTEQIT
                     DLLVKELKPKIKNVSVDEVFYYGTGCANPANAKSVKKAITNVVPGAKVAVTHDLMAAA
                     RALCGRKKGIACILGTGSNSCYFDGKKIVKNSPGLGYVLGDEGSGAYLGKKVIQYYLY
                     GTFDDELMGRFDVTYTTNKAEILENVYKKPLPNRYLAGFARFLADNRGHYMIENIIED
                     GLNDFFFNHLCKYRETWTLPVSFAGSVAFGFKDVLQDLCNSYEFELGRVMKNPMEGLL
                     EYHS"
BASE COUNT         1204 a          954 c          876 g         1313 t
ORIGIN      
        1 cattcaccag catcaggccg tttacccagg agttattaaa gaatttgctg aaatcaactt
       61 tactcactgt tttagaaatt tcagattctt ctcctgtagg agcaaaggcc atttgcccaa
      121 aaccatagat aaggaaacca accagcatgg caataaagaa gaaaccaata ccccagatga
      181 tctttttatt gatatatgtt ttggtggccg gggcaatatg cagacgggca atttcttcca
      241 ttacattttt agtaaaccgg agagagggtt cttccagttc ggaggagcgg agcatttcgt
      301 tcacttccag caattcacgg tatttcgttt tccaggctgc atctgtttcc agcaattttt
      361 caattactga tttttcggat gcctctgcag ttccgtcaat gaaactccag agcctatcct
      421 ctatattttg ttgtgtttgc atacaacttg gtttaaatca gtttaaatct tttacttctt
      481 ctgcaaaata tgtttccatt ttttccttta gccttgttct cgccctgtat agacggactt
      541 tggctgcatt tatttctatc cccagtatct gggctgtttc ttccaatgac tgttcagctt
      601 tatagaacag ggtaatgatt tgtgcatcat ccgggttcaa catggcaatt gcttcattca
      661 ccattagcat ttttgatttt tgttcgatca tgttggcgcc cattcctgac tctttgctat
      721 cggccacttc aaacactttt tcgttatcca atgaatgaat ttccaccttt ttctttctca
      781 aaaagctgat ggcggtatta ttaacaatcg tatacagcca ggtgctgaat ttggaggccc
      841 ctttaaagtc tcccaggtat ttataagcct taataaaaga atcctgggcc acctcttctg
      901 catcttcccg gcttttcacc attctcagcg ctaatgtgaa aacgtagatt tggtaccggc
      961 ttaccaaacc ggcataagcc tgttggtctc cactcagcac tctgctaatg agttcattat
     1021 cattcagtcc ggttgacatc tgtttataag actacgtttt agtaattcag gttacagtac
     1081 aaaatacggt tatttaacaa ccggtttcaa tgccatatat atgtgaaaga gggggctgaa
     1141 agttttttgg cactgcctgt aaccacattt ctatgtgggt agtcatagat gatgtaaccg
     1201 attatgaaaa aaagttcttg aaaggctgta acctggttga aagacttata gtcagaaaga
     1261 acaaacacga tcaattttaa aaaactataa aacgcacaat tatgcaagcc gcagaagctt
     1321 tagtaccaat cacaatgttt gccggtggat ttgccatggt gtttggcatc gcatacctta
     1381 aaacccgtga aaacatggcc atgatagaga aaggaatgaa cccgaaggat aaaactaacc
     1441 gtcctgcacc tttcaaaagc ctgaaagcag gattgttatt ccttggtgcc ggtcttggtt
     1501 tgttaaccgc ttttttcctg gatatgtatg tgatcccgca tgaatttgag cctgttggcc
     1561 tttatttcgc ccttattgcc atcggcggtg ggttgggatt aattggttct tattccgtgg
     1621 aaaggaaaca ctggtacgat aaggcaaata aatagaaaca ctaaaacata cacaaacctt
     1681 gtcccgaagc aatggcgaaa accattgcta ttttatttgc tgattacttc catggctttg
     1741 cggacggcca tatcgtcata atttaaaacc tggtaaaagc cggtattccg ccatttgaaa
     1801 cgggccagta aagcttttag cctaagttgc agcatttctt ttttgcggcc attgatggtt
     1861 gataagttta cagaatctgt tatcgcggct tttacccatc ctgcccacat ctctgtaaca
     1921 gcattaaagc ctttggcata ctcatcagat gatttgtact ggtcaatgtg ctgtttatgc
     1981 tgcaggtaat aattataaac atagttattc atgctgttgc ccgtcagcag tccgttcagc
     2041 tcacggtggt ataaagttgt gtctaatggg atgaaaacag taggggtgat gccatcgccg
     2101 gcatataatg tatccttaca ggatgtgagg aatttcttac ctccattatg tcttttaatg
     2161 gaatcatagg aaaccatttc tccgctgttg aagcgttccc agagatcatc catgtaaact
     2221 ttctttcctt tttcgtaaga ccgctggata ctgcggccaa gcggtgtata atacctggct
     2281 acagtaagcc tgatggctgc gccgtcactt aaatcatact gctgttgtac caggcctttt
     2341 ccaaaggttc tgcggccaat gatagtagcc ctgcaccaat cctggaggca accggcaatt
     2401 acttcgctgg cactggccga aagctcatct accagtaaaa ccagcttacc tgtttcgaac
     2461 tgaccgggcc ttttacagcg atactcaacc tttttactat tgactccctc cgtataaaca
     2521 atcagcttgt caccttccaa aaactcatcg gcgatgtcca ccgcttcgtt cataaaaccg
     2581 cccccatttc cccgcagatc aaacacaagc cctttcagcc cttgcttttt caggttctcc
     2641 aaagcttcca taaactcctc gtagctgttt tctgtaaaac gatttagttt gatgtaaccg
     2701 gttgttttat caagcatata ggtggcatct accgaagaaa cagggatagt gccccgggtg
     2761 acggttaaca tttttaaagc accagctctg attatctgca aatttgcttt tgagcctctg
     2821 ggcccacgaa tgtatttttt gatatcatct acctgcattt tgcgacctgc aatggaagaa
     2881 tcattcacct tgagaatttt atcaccgatc atcaggcctg ctttatcact gggcccgccc
     2941 ggaatcacat ataccacatt taccgtatca ctgaannnnn nnnnnnnnnn nnnnnnnnnn
     3001 caacgcctat tccctggaaa ttgcccgcca ggtcttcgtt cgcctctttt agatcgaccg
     3061 gtggcaggta cactgaatga ggatccagtt cgttcatcat ttcctgtatg gctttgccct
     3121 ccaaagaatc tatacgtaca gaatccacat atttcagcct gatgaggtca agtgcctcct
     3181 gcagggaact tcgtttgttg gatgaaaaaa atttgctttt accgccaccc tggcttccca
     3241 gtttatatcc cagcatcata ccggttatca tcaccagtga aaatatcaac ggaagccaaa
     3301 cctgtaattt tttattcccc atcataaccc tttattatgt aatttgacgc aaaatagata
     3361 aaagttgctg gtttcggatt ttacccggag atgaatgatg aaaggcaaca acaatgaatt
     3421 atggcttcca ttaaactgat agctgatagt ggcgccacca aggctgaatg gtgcctggta
     3481 aacggaggaa aggttaaaac aatttttaca caaggcatca gcccttattt cctcaatact
     3541 gagcaaataa ccgacctgct ggtaaaagaa ctgaaaccaa aaattaagaa tgtttctgtt
     3601 gatgaagtct tttattatgg taccggttgt gccaatcctg caaatgccaa atctgtaaaa
     3661 aaagccatca ccaatgtagt tcctggagca aaggtggctg tcacccacga tctgatggcg
     3721 gctgcaaggg ctttgtgcgg acgaaaaaaa ggaatagcct gtatactcgg cacaggttcc
     3781 aactcctgtt attttgatgg aaagaaaatt gtaaagaata gtcccggttt gggctatgta
     3841 ttgggcgacg aagggagcgg agcttatttg ggtaaaaaag taattcaata ttacttgtac
     3901 ggaacttttg atgatgaatt gatgggccgt tttgatgtta cttatactac gaacaaagca
     3961 gaaatactgg agaatgttta taaaaaacct ctgcctaacc gttacctagc cggttttgcc
     4021 cgtttcctcg cagacaaccg gggccattat atgattgaaa acatcattga ggatggttta
     4081 aatgattttt tctttaacca tctctgcaaa taccgggaaa catggacatt gccagtaagt
     4141 tttgcaggca gtgtggcatt tggttttaag gatgtgttgc aagacctttg caacagttat
     4201 gagtttgaat taggaagggt aatgaagaac ccgatggaag gattattgga atatcacagt
     4261 taaagaacgg cagtatgtca tttcaaaaaa ttaccgaaca gccaagctat taccgccacc
     4321 ttgaaaaaat gtcggttggc gaactgctta gcaatatcaa taaagaagac aa
//
LOCUS       DAORLS010000255         4346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11045_length_4346_cov_1.986017, whole genome shotgun sequence.
ACCESSION   DAORLS010000255 DAORLS010000000
VERSION     DAORLS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11045_length_4346_cov_1.986017"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PK977_15645"
     CDS             <1..653
                     /locus_tag="PK977_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HRF19608.1"
                     /translation="TEGNEWIPKVAKDMGIKTLVGAWLGNDDEINQREVEGLIKLAKE
                     GLVDIAAVGNEVMYRKDLSEDQLLEFIRKVKAEIPGIPVGYVDAYYEFSHRPAITELC
                     DVILCNCYPFWEGCPFENSLDYMKQMYYQAKAAGNGKEVIITETGWPSMGESLKGAIP
                     SEKSARDYFINTQLWSSNDNIPVFYFSSFDESWKVGAEGIVGAYWGIWDKNEKLKF"
     assembly_gap    759..872
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <873..1581
                     /locus_tag="PK977_15650"
     CDS             <873..1581
                     /locus_tag="PK977_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HRF19609.1"
                     /translation="KIMQGAFIEAEMNNFGCPWGGGVYEEEQLEKNKQLNLQKIKKLI
                     QLANQYPEIIFSLSVGNEACVDWTDHYVPVNKVMEYAQLVKQSAKQPVTFCENYVPWL
                     NKLKPLAGIVDFISIHTYPVWEYRHIHESIDYTRENYESVSGLYPGKPVVITEAGWAT
                     NSNGRGIHPENVNEENQKMYFEDLMGWSQNTKVLTFFFEAFDENWKGSPDPDEPEKHW
                     GLYYANRRPKLAMQTVK"
     gene            complement(1611..2978)
                     /locus_tag="PK977_15655"
     CDS             complement(1611..2978)
                     /locus_tag="PK977_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF19610.1"
                     /translation="MPSNLSRRDAIKALSFAAASFPLSSWAMNEKGERIILPDNPLHK
                     KLKKPVTAITLGAGSRGNVYGNYAVQYPDQLDIVGVAEPVQIRNERYTKKHSIPEQNR
                     FNTWEDVFKRPKFADAVIISTPDNLHYGPCMEALKMGYDVLLEKPISPSEKECRDILQ
                     LAKKTGRIVAVCHVLRYAPYFIKLRELMNSGAIGEVISMQHLEPIGHIHMSHSYVRGN
                     WHNSKQTTPIILAKSCHDLDILKWMINKPSQKIQAFGDLKWFRKENAPDGSTERCADG
                     CKIEADCPYSALRIYHRKRIWLHVFDMPEDKNKHGDHILEKLKTTNYGRCVYRMENDQ
                     PDHYICNIQFAGNVTASFSMEAFTSYEGRRTRVMGSMGDIVGDMNSFVLTDFRTGKKT
                     EWKQESDGHGGGDWRLVADWIQAIDQQNPALLTSTIDASIESHVMGFAAEKSRKENKV
                     VEVRM"
     gene            complement(2989..>3518)
                     /locus_tag="PK977_15660"
     CDS             complement(2989..>3518)
                     /locus_tag="PK977_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF19611.1"
                     /translation="TGGTLALKLAAEFPEIAGLVLLSPNIAINDPNAWLLNNHWGLQI
                     AHLVTGKYRTVADTTAIYAQYWNNRYVTKSLVQLEELLETTMKESTFKKITQPVLLLY
                     YFKDEEHQDPVVKVSAMKRMFRQLGTPENRKREVPVPNAGDHVIGSYIKSKDHQTVLA
                     ECEKFATEVLKMYQR"
     assembly_gap    3519..3621
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3622..3977)
                     /locus_tag="PK977_15665"
     CDS             complement(<3622..3977)
                     /locus_tag="PK977_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF19612.1"
                     /translation="MKFLKGLGIFLLLLIIVYFLGPKPSTPRYHKELPDVPAEAIALE
                     QYIKKEEAKHKIKPDNEARIIWYDDSVKQKTEYAIVYLHGFSASQEEGDPVHYEFAQK
                     FGCNLYLSRLDAHGIDT"
     gene            4156..>4346
                     /locus_tag="PK977_15670"
     CDS             4156..>4346
                     /locus_tag="PK977_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF19613.1"
                     /translation="MGYQNFEELEVWKKARELKNEISQLVKKFPSEEKYRLTDQLIRS
                     SRSINTQISEGHGRRTWPD"
BASE COUNT         1169 a          804 c          918 g         1238 t
ORIGIN      
        1 gtaccgaagg aaatgaatgg atacccaaag tggcaaaaga catgggtatt aaaacactgg
       61 taggcgcctg gctgggtaat gatgatgaaa ttaaccagcg ggaggtggaa gggttgatca
      121 aactcgccaa agaaggcctg gtggatattg cagccgtggg taatgaagta atgtatagaa
      181 aggaccttag cgaagaccag ttgctggagt ttataagaaa ggtcaaagcg gagattcccg
      241 gcataccggt tggttatgtg gatgcctatt acgagttttc acaccggccg gccattacag
      301 aattatgtga tgttattctc tgtaactgtt atccgttctg ggagggttgt ccgtttgaga
      361 attccctgga ttatatgaag cagatgtatt accaggcaaa ggcggctggt aatggcaaag
      421 aagtaatcat tacagaaaca ggatggccca gcatgggtga gagtttgaaa ggcgccatac
      481 cttctgagaa gagtgcaaga gattatttta tcaatacaca gttgtggtca tccaacgata
      541 atatacctgt gttttatttt tcttcttttg acgaatcatg gaaggtaggc gcagaaggaa
      601 tagtaggcgc ttattggggt atttgggata aaaatgaaaa actgaaattt taagcatgga
      661 tttttttgaa cagtttcaat tacctgttgc aagtgcaata tgttattcag gattcaggga
      721 tgggcaacag ccgggagccg gttatccgtc ttatgatgnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaaaatta tgcaaggggc ttttatcgaa
      901 gcggagatga ataatttcgg ctgtccctgg ggcggagggg tgtatgaaga agaacagttg
      961 gagaagaaca aacagctaaa tcttcagaag attaaaaagc taattcagct tgccaatcaa
     1021 tatcctgaaa ttattttctc tttatcggta ggaaatgaag cctgtgtaga ctggactgat
     1081 cattatgttc cggtaaataa ggtgatggag tatgcacaac tggtaaagca atctgccaaa
     1141 cagccagtta ctttttgtga gaattatgtg ccctggctga ataaactcaa gccgcttgca
     1201 ggtattgtgg attttatttc aatacacact tacccggtat gggaatacag gcatatccat
     1261 gaatccattg actataccag ggaaaattat gaatcagttt caggcttata tcccggcaag
     1321 ccggtagtta ttacagaagc cggttgggcc accaactcca atgggagggg gattcatccg
     1381 gagaatgtaa atgaagaaaa tcaaaaaatg tattttgaag atttaatggg atggtctcaa
     1441 aatactaagg tgctcacttt tttctttgaa gcgtttgatg aaaactggaa gggttcacca
     1501 gatcctgatg aaccggagaa gcattgggga ttatattatg ccaatagaag accaaaattg
     1561 gcgatgcaga cagtgaagtg agatttttaa agagaaataa ggaaatttta ttacatcctc
     1621 acttctacca ccttattctc tttccggctt ttttctgctg cgaatcccat tacatggctt
     1681 tcaatggaag catcaatcgt tgaagtaagt aaggcagggt tttgctggtc aatggcctgt
     1741 atccagtctg ccaccaaccg ccagtcaccg ccgccatgtc catcgctttc ctgtttccat
     1801 tctgtttttt tgccggtacg gaaatcagtt aatacaaatg aattcatgtc accaacaata
     1861 tctcccatgc tgcccattac cctggttctt ctgccttcat aggaagtaaa ggcttccatc
     1921 gaaaaactgg cagtaacatt acctgcaaac tggatgttgc agatataatg atccggctga
     1981 tcattttcca tccggtacac acaacggccg taattagtgg tttttagttt ttccaggata
     2041 tgatctccgt gtttgttctt gtcttccggc atatcaaata catgcaacca aattctttta
     2101 cgatgatata tccgtaaagc tgagtaagga caatcagctt ctatcttgca tccatcggcg
     2161 catcgttcag tacttccatc cggtgcattt tctttccgaa accacttcag atcgccaaaa
     2221 gcctgaattt tttgagaagg tttattgatc atccatttca gtatatccag gtcatggcag
     2281 gatttggcaa ggatgattgg agtagtttgt ttgctattgt gccagttgcc ccgtacataa
     2341 gagtggctca tatgtatatg gccaatcggt tccaggtgct gcatactgat tacttccccg
     2401 atagcaccac tgttcatgag ctctctcagc ttgataaaat agggtgcata acgcagcaca
     2461 tggcagacag ccacaatcct cccggttttt ttggcgagtt gcaaaatatc ccggcattct
     2521 ttttcactcg gtgagattgg tttttccagc aacacatcat agcccatttt caaggcttcc
     2581 atacagggtc cgtaatgtag attatcgggt gttgatataa taactgcatc agcgaatttg
     2641 ggtcgcttaa aaacatcttc ccaggtgttg aacctgtttt gttcaggtat gctgtgcttt
     2701 ttagtgtaac gttcattccg gatttgaaca ggttctgcca caccgacaat atctaattga
     2761 tcaggatatt gcacagcata attaccatat acattacctc ttgaccctgc accaagggtt
     2821 atggcggtta ccggcttttt taatttttta tgcaaagggt tatcaggcag aatgattctt
     2881 tcgccttttt cattcattgc ccatgatgaa agaggaaatg aagctgcagc aaaactcagt
     2941 gcttttattg catcacgacg ggaaaggtta gaaggcataa aaaacgtttt agcgttgata
     3001 catttttaga acttcagtgg caaacttttc acattcggcc agtacggtct gatggtcttt
     3061 tgatttgata taagaaccaa tcacatgatc accggcgttg ggtacaggca cctctctttt
     3121 cctattctca ggtgtaccta actggcgaaa cattcttttc atggctgaca cttttaccac
     3181 aggatcctgg tgttcttcat ctttaaaata atacaggagt aatacaggtt gagtaatttt
     3241 tttgaaagtt gattccttca tagttgtttc cagcaactct tctaactgta ctaatgattt
     3301 tgttacatac cggttgttcc agtattgcgc ataaatagca gtggtatcgg ctacagttcg
     3361 gtacttacct gttaccaggt gtgcaatctg taagccccaa tgattattta gcaaccaagc
     3421 gttaggatcg ttaatggcaa tattcggtga aagtaaaacc aggccggcta tttcaggaaa
     3481 ttctgcagct agtttcaatg ccagtgtacc acccgtggnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn ngtatcaata ccatgtgcat ccagccggct caggtataaa
     3661 ttgcaaccaa acttttgggc aaactcataa tgcaccgggt cgccttcttc ctgcgaagca
     3721 gaaaagccgt gcaggtatac aatagcatat tctgtttttt gttttacgga atcatcatac
     3781 cagatgattc ttgcttcatt atctggtttt attttatgct ttgcttcttc ttttttgatg
     3841 tattgttcaa gagcaatggc ttcggcgggt acatccggca gttctttatg gtacctgggc
     3901 gtagatggtt tgggtcctaa aaagtaaaca ataattagga gtaaaaggaa gatacccaag
     3961 cctttgagaa atttcatagc cttgattttc ctaaaactac gccaaatccc tgtttaaagc
     4021 ggcattaagg ctgtattaaa tacctgtttc gtttgctgcc cccgctttat taacgtacct
     4081 ttgccggccg gaaaagttga taggttgaac agtttaaaag gaaaaaccct cattttcaga
     4141 cgcttaatta aaattatggg ctaccagaat tttgaggaac tggaagtttg gaaaaaggca
     4201 agagagttga agaatgaaat ttctcagtta gtcaaaaaat ttccgtcaga ggaaaaatac
     4261 aggttaactg atcaacttat tcgtagttca aggtcaatta atacacagat ttctgaaggt
     4321 catggacgaa ggacctggcc ggacag
//
LOCUS       DAORLS010000256         4320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11130_length_4320_cov_2.069871, whole genome shotgun sequence.
ACCESSION   DAORLS010000256 DAORLS010000000
VERSION     DAORLS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11130_length_4320_cov_2.069871"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1343
                     /locus_tag="PK977_15675"
     CDS             120..1343
                     /locus_tag="PK977_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF19614.1"
                     /translation="MHFLFAWRYFKAKKSTNAINIIAWVSIAAILIGTTALILVLSVF
                     NGFEGLVKSLYSSFYTDLKVSAASGKIITVNQEQLNQLRGINGIRNFALVVEEKALVQ
                     NGENQSVIHLKGVDENYRYITGVADNILVGQYNIGTADSPKIILGIGVEGALQVSSDA
                     YTTALRIYLPRKGGTEQLNMLEDISSNNVLFSGSFRIQQDFDNKYGITSINFLKQAMQ
                     MGANDYNGIEIAVTDPSKTAEIKRKVKKIFGETYLVQNKYEQNRSLYSIMNVERWVIY
                     GVLCLILVVAAFNMIGALSMLVLEKQKDISVLHAMGANKGFIQKIFLSEGLLLAIIGG
                     GIGMLLALVIALLQLKYHLIPLQGGSFLINYFPVQLKFTDFLLVGTTVFVIAMLASWL
                     PSRKAASQDFSLRSE"
     gene            complement(1350..2183)
                     /locus_tag="PK977_15680"
     CDS             complement(1350..2183)
                     /locus_tag="PK977_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRF19615.1"
                     /translation="MAELTAIASQIRRDIVRMVHGVNSGHPGGSLGCADFLTALYFKI
                     MKHNPDFSMDAKEEDVFILSNGHISPLYYSTLARAGYFDIKELGTFRKLNTRLQGHPA
                     THEHLPGVRIASGSLGQGMSVAIGAALTKKLNGESNLVFSLHGDGELDEGQIWEAVMF
                     AAHHKVDNLISTIDWNGQQIDGPTDKVMGLGNIREKFDVFGWQTLEMNGNNMDEVVAT
                     LEKAKTLTGKGKPVAIMMHTAMGKGVDFMEGTHEWHGIAPNDDQLAKALAQLPETLGD
                     Y"
     gene            2428..>2910
                     /locus_tag="PK977_15685"
     CDS             2428..>2910
                     /locus_tag="PK977_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRF19616.1"
                     /translation="MLDILLTASVSFIITFLAIPVVIQVAEKKKLYDIPDERKLHTRL
                     VASLGGVGIFGGFILASLLSIQGYQNPEFQYFFAAAIIIFFLGLKDDIMILSASKKFV
                     GQIIAASILIHLGGIRLDSMHGLLGFNELPEGFGLALSYLTIIVVINSFNLIDGVDGL
                     A"
     assembly_gap    2911..2983
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3141..>3332
                     /locus_tag="PK977_15690"
     CDS             3141..>3332
                     /locus_tag="PK977_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRF19617.1"
                     /translation="MAVPVQSAVAVGFAILLVPLLDTLRVFGIRIFKGRSPFTPDRNH
                     VHHLLLDSGLNHAAVTFTCV"
     assembly_gap    3333..3446
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3703..4266
                     /gene="frr"
                     /locus_tag="PK977_15695"
     CDS             3703..4266
                     /gene="frr"
                     /locus_tag="PK977_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRF19618.1"
                     /translation="MAEDIASIFADAEDHMKKAINHLEAELVKIRAGKANPQMLDGLV
                     VDYYGSPMPINQVANISVMDARTLSIQPWEKNMLQPIERAIIAANIGITPQNDGALIR
                     LFLPPLTEERRKELVKKCHGEGEHTKVAIRNIRRDAIEHIKKLQKNGLSEDAAKDAEA
                     DIQQVTDKYTAAVEKHLTAKEKEIMSV"
BASE COUNT         1199 a          815 c          860 g         1259 t
ORIGIN      
        1 attgaagttt ttccatgatg ataccctgga tcatgttttc aaaatggagg agttgttcaa
       61 gaagattgag gaggaaaaga aatcccggcc tgattaatta atcaaaatca ccagatacct
      121 tgcacttcct ttttgcctgg agatacttta aagccaagaa atcaactaat gcgatcaata
      181 tcattgcatg ggtaagtatt gccgcaatct taataggaac tacagcattg atactggtac
      241 ttagtgtgtt taatggcttt gaggggctgg ttaaatccct ttattcttca ttttatactg
      301 atttaaaagt ttcagctgct tcggggaaaa tcatcacggt aaaccaggag cagttgaacc
      361 agttgcgagg cattaacggt atccgaaatt ttgcgttggt ggtggaagaa aaggcgctgg
      421 tgcagaacgg agaaaatcaa tcagtcattc acctgaaggg ggttgatgag aattatcgct
      481 atatcacagg tgttgctgat aacatactgg taggccaata caatattggt actgcagact
      541 cgccaaaaat catattgggc attggcgtcg agggagccct gcaggtaagt tccgacgctt
      601 atactacagc attgcgtata tacctgcccc gtaaaggagg aacggaacaa cttaatatgc
      661 tggaggatat aagcagtaat aatgttttgt tctcagggtc tttcaggatt cagcaggact
      721 ttgataataa gtatggaatt accagtatca attttttaaa gcaggctatg cagatgggcg
      781 ccaatgacta caacggaatt gaaatagctg taacagatcc ttccaaaact gcggaaatta
      841 aaagaaaagt aaaaaaaata ttcggcgaaa cttacctggt acaaaacaag tacgaacaaa
      901 accgcagtct ttattctata atgaatgttg aacgttgggt gatctacgga gtgctttgtc
      961 ttatcctggt ggtggccgca tttaatatga taggagcctt atctatgctg gtacttgaaa
     1021 aacaaaagga tatcagtgta cttcatgcta tgggggccaa taaaggtttt atacaaaaaa
     1081 tattcttaag tgaaggttta ttgctggcca tcattggcgg agggattgga atgttgctgg
     1141 cacttgtcat tgcattactg caattaaagt atcatttaat cccgttgcag ggaggttcat
     1201 tccttatcaa ttatttccct gttcagttaa aatttacaga tttcctgtta gtgggtacca
     1261 cggtttttgt gattgccatg ctggcttcgt ggcttccatc ccgcaaagct gcttctcaag
     1321 atttttcttt acgaagtgaa tagtaataat cagtaatctc ccaatgtttc cggtaattgt
     1381 gccagcgctt ttgccagttg gtcatcgttg ggtgcgatac catgccattc atgggtgcct
     1441 tccataaaat caacgccttt gcccatagct gtatgcatca taatggcaac aggttttcct
     1501 tttccggtga gggttttggc tttttctaaa gtggctacca cttcatccat gttgttacca
     1561 ttcatttcca gtgtttgcca gccaaataca tcgaattttt ccctgatatt accaaggccc
     1621 attactttat cagttggtcc gtcaatttgc tggccattcc agtcaatggt ggaaataagg
     1681 ttgtcaactt tatgatgagc agcaaacatt acagcttccc agatttgtcc ttcatccaat
     1741 tctccatcgc catgcaagga aaaaaccagg ttgctttctc cattcagctt ttttgtcaag
     1801 gctgcgccaa ttgccacact cattccttgt cccaaagaac ctgaagcaat acgaacaccc
     1861 ggtaaatgtt catgggtggc tggatgtcct tgcagtcttg tatttaattt tctgaaagtg
     1921 ccaagttctt tgatatcaaa atacccggcc ctggccagcg ttgaataata aagaggtgag
     1981 atatgaccgt tagatagtat aaatacgtct tcctcttttg catccatcga gaaatcagga
     2041 ttatgtttca ttattttgaa ataaagggct gtcaaaaaat cagcacagcc caatgaacca
     2101 cctggatgtc cgctgtttac accatgtacc atacgaacta tatcccttct tatctgtgat
     2161 gcgattgcag ttaattctgc cataagcttt gatattatta aactttttat acttttgtaa
     2221 ctctaacgct tcagaagcac tgatgcgctg caaaagtgca ttcttttttg atataaaatc
     2281 caaccggatt aatcaaactg ccaacaaagt caaatcatcc tgtggtttta caattaatta
     2341 attgagccat ttaataaata gtggcataat tatatagaaa cccggaagtt aaaacctaat
     2401 tttgtgaccg attaaacctt gcttaggatg ctcgatatat tgctgactgc ttctgtttct
     2461 ttcatcatca cctttctggc gatccctgtg gtaatacagg tggcggagaa aaagaaacta
     2521 tatgatatac cggatgaaag aaagcttcac acaagattag tggcgtcttt gggcggagtc
     2581 ggtatttttg gaggtttcat tttagcttct ctattatcca tacaggggta tcaaaaccca
     2641 gaattccagt acttttttgc tgcagccatc atcatttttt tccttggact gaaagatgac
     2701 ataatgattc tttctgccag caagaaattt gtgggtcaga ttatagccgc atccatactt
     2761 atacatctgg gtggtatccg tctcgatagc atgcatgggt tattaggttt taatgaatta
     2821 ccggaaggtt ttggcctggc tctttcatat cttacgatca tagtagtaat caactctttt
     2881 aaccttattg atggtgttga cggattggct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattgatg gcatttgcca
     3001 tggctggtag cttggtagcg ttccttattt tcaaccatag tccagccaag attttcatgg
     3061 gcgattctgg ttccctgatg attggtttga tcaattcgat tttagtcatt aagttcatta
     3121 atgtttctaa tacaccgttt gtggctgtac ctgtacaatc tgccgtggca gttggatttg
     3181 ccatactatt agtacccttg ctcgatacgt taagggtatt tggtataagg attttcaaag
     3241 gcagatctcc ctttacccct gacagaaacc atgtacatca cttactgctt gacagcggtc
     3301 tcaatcatgc cgcagtaaca ttcacctgtg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnngatt gggtgttttg ttttatcgca acagatcgag
     3481 ccgtaaaatg gttatatcta aaagaaaaga tggggctacg gaactcaaaa ctcactcaaa
     3541 agtggttacc ctgaccaaag aaacccctgc cgcggggaat aattaatcat tttaaataaa
     3601 aaatgaatta ctgccttttt tgaacccttt ggcaggtaaa ttcttctgtt tcattagctt
     3661 tgcctccctg taactattac agttaactaa aaacgtacag atatggcaga agatatcgcc
     3721 tccatttttg cagatgcaga agaccacatg aaaaaagcga tcaaccacct ggaagcagaa
     3781 ttggtaaaaa taagggccgg caaggccaat ccccagatgc ttgatgggct ggtagttgat
     3841 tattatggtt cacctatgcc cattaaccag gtagccaata tcagtgtgat ggatgcccgt
     3901 acactgagta tacaaccgtg ggagaagaac atgctgcagc ccattgaacg ggccattatt
     3961 gctgctaata ttggcataac accacaaaat gatggtgccc ttatccgttt gttcttacca
     4021 cccctgactg aagaaagaag gaaagaactg gtaaaaaaat gtcatggcga aggtgagcat
     4081 actaaagtgg ccattcgtaa tatccgccgt gatgcaatag aacatatcaa aaaactacag
     4141 aaaaatggtt tgagtgagga tgccgcgaaa gatgctgaag cggacattca gcaggttacc
     4201 gataaatata ctgctgctgt agaaaaacac cttacagcta aggaaaaaga aataatgtcc
     4261 gtttaattct ttagaaaaaa taataattga tgggcagtac ttattcggtg ctgcccactt
//
LOCUS       DAORLS010000257         4315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11150_length_4315_cov_2.312676, whole genome shotgun sequence.
ACCESSION   DAORLS010000257 DAORLS010000000
VERSION     DAORLS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11150_length_4315_cov_2.312676"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK977_15700"
     CDS             <1..324
                     /locus_tag="PK977_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19619.1"
                     /translation="YPLPDSKPVVIPVQMNNPRLVITDGYHISRPLKMVYKDLHTYCF
                     KVTCAVSDGQLLGGFIGLAGMYLSGFYTGLFLLKILSFLPLVYLLLFYYLNRKDFFRL
                     VPVLN"
     gene            409..1017
                     /locus_tag="PK977_15705"
     CDS             409..1017
                     /locus_tag="PK977_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRF19620.1"
                     /translation="MAKLLILFAHPALEKSRIHKGMIRHISQIENVTMHDLYEEYPDF
                     DIDVFKEQKLLLQHDVIIWQHPFYWYSAPAIIKQWMDLVLEHGWAYGENGKMLQKKKV
                     FNAISCGGSKEVYSPTGRNRFSISQFLVPFDQTAALCGMLYLPPFVVHGTHRLEKGDI
                     ELYSLQYEQLITAFTNDRIQEEEWKSVTYLNELCPIPESIKS"
     gene            1020..>2165
                     /locus_tag="PK977_15710"
     CDS             1020..>2165
                     /locus_tag="PK977_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRF19621.1"
                     /translation="MDQNSFFFQAMVYLASAVLMVPLAKRLGLGSVLGYLLAGILIGP
                     ALLGFIGNEGEDILHFAEFGVVMMLFVIGLELEPELLWRLRKSIIGLGGLQVLITALL
                     IAAVAMLIGLDWRQSLALGMILSLSSTAIVLQTMNEKGLMKTAAGQSSFAVLLFQDIA
                     VIPMLALFPLLSVQQPVAVADDNHTRTLVEGLPGWAQTLIVLGSVAAIILAGRFLIRP
                     VFRIVARTRLREVFTATALLLVVGIAVLMSQVGLSPALGTFLAGVVLADSEYRHELES
                     DIDPFKGLLLGLFFIAVGASIDFNLVMEQPLLIAGLVLGLMLLKALVLLFLGRIFKLG
                     VDQNMLFAFALCQVGEFAFVLFSFSLQEAILPKETVDIMVAVVAISMG"
     assembly_gap    2166..2185
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2186..2951
                     /locus_tag="PK977_15715"
     CDS             <2186..2951
                     /locus_tag="PK977_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRF19622.1"
                     /translation="GLTPLVLLINEKLIQPYFGTKEAEPKEADYIDEKNRVIIAGFGH
                     FGNTVGRFLRAHGVGLTILDVDSDRVEVLRKMGFKVFYGDASRYDLLHTAGAAEAKLI
                     IIALEPPDKRLAMIETIKKHFPNLRMYVRAENRFDAYDLMNAGMLHVYRETIDTSLRM
                     GVDVLKNLGVRSYTAHRAARIFFKKDESNLKKLASIKDEEEYINTAREYIEELETILS
                     NERKDNILGNDMSWDEESLIEEARSKMKEEKISGTK"
     gene            2938..4107
                     /locus_tag="PK977_15720"
     CDS             2938..4107
                     /locus_tag="PK977_15720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19623.1"
                     /translation="MEQSRKKILWLCSWYPSKIDTFNGDFIQRHARAAAAFNDIYVIY
                     VAGDNSGKINKKEKEIRKSDGLTEHLVFFPVSSSFFGKIRAHFKYLFVFKQAIRRYIV
                     EIGKPDLVHVQVPIKAGLLALWLKKRYRIPFIITEHYGIYNEETGSDSYLNRSKIFKN
                     YTRRIFQQASGFVSVSKFLAEGVKRMVGKIKYVIVPNTVDTNIFFQKQKKQTDFRFIH
                     VSNMVPLKNAEGILRSFKSYTEGGGTATLHMVGNLNDILPRYAASLGLLSDKVCFKGI
                     VSYQQVASEMQESDCLILFSNIENSPCVIGEALCCGLPVISSNAGGIPELVNSENGIL
                     VNPGNEDELLTAMHQVASGNIQFDRKKIAEKATAKFSYSVIGKELDDIYNEILNP"
     gene            complement(4097..>4315)
                     /locus_tag="PK977_15725"
     CDS             complement(4097..>4315)
                     /locus_tag="PK977_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HRF19624.1"
                     /translation="SKALPEKPSNETLLQLYALYKQSTEGDNNSEPPSNPFDFVGKAK
                     YEAWAALKGKSLTEAMTEYIELVKKLKG"
BASE COUNT         1244 a          726 c          971 g         1354 t
ORIGIN      
        1 tatccgttac ctgatagcaa accagtggtt atacccgtgc agatgaataa ccccaggctc
       61 gtaattaccg atggttatca tatctctagg ccattaaaaa tggtttataa agatttgcat
      121 acatattgct tcaaggttac ctgtgcagtt agtgacggcc aactgttagg tggttttatt
      181 ggattggctg gcatgtatct ctcaggtttt tatacaggtt tatttctttt gaagatactg
      241 agttttcttc ctcttgtcta cctgcttctt ttttattacc tcaacagaaa agattttttc
      301 aggctggtgc cggtattgaa ctaataatat aaaaggttgt ttcatctgtt gtataagtat
      361 tcatgtccat tcttttgaaa actttctgaa atccattaaa cttgtcatat ggctaaactg
      421 cttattttat ttgcacaccc ggcattggaa aaatcgagga tacataaagg tatgatccgc
      481 catattagtc aaatagagaa tgtgacaatg catgatttgt atgaagaata ccctgacttt
      541 gatatagacg tatttaaaga gcaaaaattg ctcttacagc atgatgtcat tatatggcaa
      601 catccctttt attggtatag tgctcctgcg attataaagc aatggatgga tcttgttctc
      661 gaacatggct gggcctatgg tgaaaacggg aaaatgttac aaaaaaagaa agtatttaat
      721 gcaatttcct gcggaggttc aaaggaggta tatagcccga caggcagaaa ccgcttttca
      781 atttcccagt tccttgtacc atttgatcag actgctgcac tttgcggtat gctctacctg
      841 cctccatttg tagtgcatgg tacccatcgt cttgaaaaag gggatattga attatattca
      901 ttgcaatatg agcaattgat cacagctttc acaaatgatc gaatacaaga agaagaatgg
      961 aagtccgtaa cctatctcaa tgaactatgc ccgatacctg aatcaattaa atcctgacta
     1021 tggatcaaaa ttcttttttc ttccaggcta tggtgtacct ggcctcggct gtgctgatgg
     1081 ttccattggc taaaaggcta gggctgggtt ccgtacttgg atacctgctg gcgggtatcc
     1141 tgatcgggcc ggcgttactt ggctttattg gcaatgaggg ggaagatatt ttgcattttg
     1201 cagagtttgg cgtagtgatg atgctatttg taattggatt ggaactggaa cccgaattat
     1261 tatggcggtt aaggaaatca attattgggt taggtggcct gcaggttttg attacagctt
     1321 tattaattgc agcggttgcc atgttaattg gattagattg gcggcagtca ttagctcttg
     1381 gaatgatttt atcgctttct tccactgcaa tcgtattgca aacgatgaac gaaaaagggc
     1441 tcatgaaaac tgcagccggg caaagttcat ttgctgtttt gttatttcag gatattgcag
     1501 ttattcccat gctggcttta ttcccgcttc tttctgttca gcaacctgtg gctgtggcag
     1561 atgataacca tacaaggaca ttggtggagg gactgcccgg ctgggcacaa acactgatcg
     1621 tattaggttc agttgccgct atcatacttg ccggaagatt tttgatcagg ccggtattcc
     1681 ggattgttgc gagaacaaga ctcagagaag tttttacagc aacagcacta ttattagttg
     1741 ttggaattgc tgttttaatg tctcaagtag ggcttagtcc ggctttagga acttttttag
     1801 caggcgttgt tctggccgac agcgaataca ggcatgaact ggaaagcgat attgatcctt
     1861 tcaaaggttt attattaggg ttattcttta ttgcagtagg ggcatctatc gacttcaatc
     1921 ttgtgatgga gcagccatta ttgatagctg gccttgttct tggattaatg ctgctaaagg
     1981 cacttgtact gctatttctt ggccgtatat ttaaattggg ggttgaccag aacatgttat
     2041 ttgcttttgc tctttgccag gtaggtgagt ttgctttcgt tttattttca ttctcccttc
     2101 aggaagcaat tttaccaaag gaaactgtgg atataatggt tgcagttgtt gctattagta
     2161 tgggtnnnnn nnnnnnnnnn nnnnngggtc tgacaccatt ggtcttgtta ataaatgaaa
     2221 agctgattca gccctatttt ggaacaaaag aggctgagcc aaaagaggct gattacattg
     2281 atgagaaaaa cagggtcatt attgccggat ttggacattt tggcaatacc gtaggaagat
     2341 ttttaagggc acatggtgta ggactaacta tacttgatgt agattcggac agggtagagg
     2401 tgttaagaaa aatgggtttc aaggtttttt acggtgatgc ctcacggtat gatctcttgc
     2461 atacagcggg ggctgctgag gcaaagctaa taatcattgc actggagcct ccagataaac
     2521 gactcgcaat gatagaaacc attaagaaac attttcccaa tctgcgtatg tatgtcaggg
     2581 ctgaaaacag gtttgatgcc tacgacctga tgaatgccgg gatgcttcat gtttacaggg
     2641 aaacaattga tacatcttta agaatgggtg ttgatgtgtt gaaaaacctg ggagtacgtt
     2701 catatacggc tcatagagct gccaggatat tttttaaaaa ggatgaatcc aatcttaaga
     2761 aactggcttc tattaaagat gaagaagaat acattaatac agcaagagag tatatagaag
     2821 aactggaaac aatattgagt aatgaacgaa aagacaatat cttagggaat gacatgagtt
     2881 gggatgaaga aagtcttatt gaagaggcaa ggagtaaaat gaaggaggag aaaattagtg
     2941 gaacaaagta gaaagaaaat attgtggttg tgcagctggt atcccagtaa aatagataca
     3001 tttaatggag attttattca aaggcatgca agagcagccg ctgctttcaa cgatatatat
     3061 gttatttatg ttgccggcga taattctgga aaaataaata agaaagaaaa ggaaatccga
     3121 aagagtgatg gcctcactga acatctagta ttttttccgg tcagtagttc tttttttgga
     3181 aaaatcagag cacattttaa atacttgttt gtttttaagc aagctatacg gcgctacatc
     3241 gtggaaattg gtaaaccgga cctggtgcat gttcaggtac cgataaaggc aggtttactg
     3301 gccttatggc taaaaaagcg ataccggatt ccttttatca ttaccgagca ttatggtatt
     3361 tataatgaag aaacaggaag tgatagttat ttaaatcgat caaaaatttt caaaaattat
     3421 acccgacgga tatttcagca ggccagtggt tttgtatcag ttagtaaatt cctggcggaa
     3481 ggagtaaaga ggatggttgg caagattaaa tatgtaatag tacccaatac tgttgatacg
     3541 aacatttttt tccagaaaca aaaaaaacaa acagacttca ggtttataca tgtgtcaaat
     3601 atggtgccat tgaaaaatgc ggaaggaatt ctgagatcat ttaagtcata tactgaaggt
     3661 ggtggcaccg cgactttgca tatggttggt aacttgaatg atattttacc caggtacgct
     3721 gcttcgttgg gtctgctttc agataaagtt tgttttaaag gcattgtatc ctaccagcag
     3781 gtagcttcag aaatgcagga atctgattgc cttatccttt tcagtaacat tgaaaactct
     3841 ccctgtgtga ttggtgaggc cctttgctgt ggtttacctg ttattagttc caatgcaggc
     3901 ggtataccgg aattagtgaa tagtgaaaat ggtattcttg tcaacccggg caatgaagat
     3961 gaattattaa cagctatgca tcaggttgca tcaggtaata ttcaatttga tagaaaaaaa
     4021 atagctgaaa aagccactgc caaattcagc tattccgtta ttggtaaaga actggatgat
     4081 atctataatg agatacttaa cccttaagtt tttttaccag ctcaatatac tcggtcattg
     4141 cttctgttaa tgattttcct ttgagtgctg cccatgcttc atacttggct tttcccacaa
     4201 aatcaaaagg attagaaggc ggctcgctgt tattgtcacc ctctgttgac tgcttataca
     4261 gggcgtacaa ttgaagcagg gtttcattgc ttggtttttc aggcaatgct ttact
//
LOCUS       DAORLS010000258         4292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11210_length_4292_cov_2.223979, whole genome shotgun sequence.
ACCESSION   DAORLS010000258 DAORLS010000000
VERSION     DAORLS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11210_length_4292_cov_2.223979"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PK977_15730"
     CDS             <1..362
                     /locus_tag="PK977_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRF19625.1"
                     /translation="LEKVADLGEIWERKMNVPIPLGGIAIKRTVDRLISLQVDQLIRK
                     SLEYAFANYPVITDYVKCHSQTMSEEVMRKHIDLYVNNYSLNLGEEGKKAIITLYNTF
                     LLMNNMVPSKNDEELFL"
     gene            complement(351..1643)
                     /locus_tag="PK977_15735"
     CDS             complement(351..1643)
                     /locus_tag="PK977_15735"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRF19626.1"
                     /translation="MNIFWFRRDLRLHDNAGLYYALRSGKPVVPVFIFDTNILGELED
                     KADRRVEFIHLALQEIQQELIKYGSTLDVRYGEPLAIYKELASEYTIEAVYTNHDYEP
                     YATKRDAAIEQLLTLKGISFNTFKDQVILEKGEVLKDDGKPYTVFTPYSRRWKTTLTP
                     FHLTSYPIEKYFSHFFKQATRQIVSLEKMGFKASGISFPPKSWKSEIIKRYKEQRDIP
                     SIEGTSRLSVHLRFGTISIRELARGAGALNESFLNELIWRDFYHMILWHFPRVVGHAF
                     KPEYDRIKWRNNETEFAAWCEGRTGYPIVDAGMRELNATGFMHNRVRMIVASFLTKHL
                     LIDWRWGEAYFAKKLLDFDLAANNGGWQWAAGSGCDAAPYFRVFNPYLQTQKFDPDLK
                     YIRKWVPEFEELTYPRPIVEHEVARKRCLETYAVALKK"
     gene            complement(1672..2511)
                     /locus_tag="PK977_15740"
     CDS             complement(1672..2511)
                     /locus_tag="PK977_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRF19627.1"
                     /translation="MATICFNGKFTDADKAVLPASNRSYRYGDGLFETMKVKDGVIQL
                     AASHMERLFDGMSILKMDVPALVTKDKLMDIILELCKQNRCEALARVRLSVSRGNGGL
                     YDEDRKLQYLVECWPLNQSVNRVNENGLVIDIFPDAAKSTDRFSNLKSANFLVYSMAA
                     VYAKENKLNDCLVLNANGKIADSTIANLFLVKGDTIFTPALSEGCVNGVMRKYLLESL
                     AASNYQVEETIVSAEDLLAADEVFLSNAINGIRWVQRFRNKIYSHMKTMEIYRRFCQT
                     IPG"
     gene            complement(2584..3258)
                     /locus_tag="PK977_15745"
     CDS             complement(2584..3258)
                     /locus_tag="PK977_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF19628.1"
                     /translation="MKNPDTNAVIKVAIADDHALFRTGIKTALQVRKDIQMVAEAENG
                     MQLLNLLRHIKPDVILLDIQMPIMDGLTALPEIKKLYPDVKVIMLSFLNDHSVISKMM
                     EMGANSYITKESGADLIYEAIRGVYENDFHFNDLTNKALLSGLRTKRTAEASMPQDVQ
                     LTDKEITILKLMCDEKSTKEIADIVDLSPRTVEAIRDKLKTKTGTKSMAGLVMYAVKA
                     GLVEQA"
     gene            complement(3854..>4292)
                     /locus_tag="PK977_15750"
     CDS             complement(3854..>4292)
                     /locus_tag="PK977_15750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF19629.1"
                     /translation="EEQFDMVICMGNSLNFFNAADTVSILKKAASFLKPGGYLLINSW
                     SLAEIVFKNSVTKSWSEINGVKFLTDAKLLFHPARLEAESTIIHPDGTTETKTGIDYI
                     FSINEMENMLQSAGFVLKEVYSIPGRKKFTLGEQRAYLVAQKQ"
BASE COUNT         1254 a          945 c          819 g         1274 t
ORIGIN      
        1 gattagaaaa agtagcagac ctgggagaaa tctgggaaag gaaaatgaat gtaccaattc
       61 ctttaggcgg aattgccatc aaaaggacag ttgacaggtt gataagttta caggtggacc
      121 aattgatccg caaaagtctt gaatatgctt ttgccaacta tccggttatc actgattatg
      181 taaaatgtca ttcacaaaca atgagtgagg aagtaatgag aaagcacatc gatctttatg
      241 taaataacta ttcactcaac ctgggagagg aaggtaaaaa agcgataata actctttaca
      301 atactttttt attaatgaat aatatggtgc caagcaagaa tgacgaagag ttatttcttt
      361 aaagccaccg cataagtttc caggcaacgc ttcctggcta cttcatgttc tactatcgga
      421 cggggatagg taagctcttc gaactccggt acccatttac ggatatattt taaatcagga
      481 tcaaactttt gagtctgcaa atatggattg aaaacacgga aataaggtgc cgcatcgcag
      541 ccgcttccgg cagcccattg ccagccgcca ttgttggctg ccaaatcaaa atccagcaac
      601 ttctttgcaa aataagcctc accccaacgc cagtcaatca ataaatgctt ggttaaaaaa
      661 ctggctacta tcattcgaac ccggttatgc ataaagcctg ttgcattcaa ttcccgcata
      721 cctgcgtcga caatcgggta accagttctt ccctcacacc aggctgcaaa ctcagtttca
      781 ttattcctcc attttatccg gtcatattct ggtttaaagg catggcccac cacccgggga
      841 aaatgccaga gtatcatgtg ataaaaatcc cgccatatca gttcatttag aaatgattca
      901 tttaaagcac cggctcccct tgccagctct cggatactga tagtaccaaa acgcaaatga
      961 acacttaatc ttgaagtccc ttctatcgac ggaatatctc tttgttcttt atagcgctta
     1021 ataatttcag atttccatga ttttggtgga aatgaaatac cactggcttt aaatcccatt
     1081 ttttccaggg aaactatttg cctggttgct tgtttgaaaa aatgggaaaa gtatttctct
     1141 atgggataag acgtaaggtg aaaaggtgtc agcgttgtct tccacctgcg ggaataaggt
     1201 gtaaaaactg tatacggctt accatcatct ttcagcactt cccctttctc cagtatcacc
     1261 tggtctttaa acgtattaaa gctgatgcct ttcaatgtca ataattgttc aatagcagcg
     1321 tcccttttcg tggcatacgg ctcatagtca tgattggtgt aaacagcttc gatagtatac
     1381 tcactggcga gctccttata aatagccaaa ggttcaccgt atctaacatc cagtgtagag
     1441 ccgtatttta ttaattcctg ctgtatttcc tgcaatgcca aatgaatgaa ctctaccctc
     1501 cggtcagctt tgtcttccag ttcaccgagg atattcgtat caaaaataaa aaccggtact
     1561 actggtttgc cactcctgag ggcataatat aatcctgcat tgtcatgcag gcgcaagtca
     1621 cggcggaacc agaatatgtt cacggcagat ttcatgatgg aagaaacagc atcagcccgg
     1681 aattgtttga caaaaacggc gataaatttc cattgttttc atgtggctat agattttatt
     1741 ccggaaacgt tgcacccaac gtatgccgtt tatcgcattg gataaaaata cctcatcagc
     1801 agccagcaga tcttctgctg aaactattgt ctcttctacc tggtagttgg aggcggctag
     1861 tgattccagt aaatattttc tcatcacgcc gttcacacat ccttctgaaa gtgcaggagt
     1921 gaaaatagta tctcctttca caaggaaaag attggcaata gttgaatctg caatcttacc
     1981 attagcattc aatacgagac aatcgttcag tttattttct ttagcgtaaa cagccgccat
     2041 tgaatacact aaaaaattgg ctgactttaa attggaaaac cggtcagtac ttttcgcagc
     2101 atccgggaaa atatcaatta ccaggccgtt ttcatttacc cgattcactg attgatttag
     2161 tggccagcac tcaacaaggt attgcagctt ccggtcttca tcatataaac ctccgttacc
     2221 tctggatact gacaacctca cccgggccaa tgcttcacat ctattttgtt tacatagctc
     2281 aagtataata tccattaatt tatcctttgt aaccaaagca ggaacatcca ttttcagtat
     2341 ggacattccg tcaaacaacc gctccatgtg ggaggctgct aattgtatca ctccgtcttt
     2401 cactttcatg gtctcaaaca aaccatctcc gtagcgataa ctcctgttgg aggccggcag
     2461 cactgcttta tcagcatctg taaattttcc attgaagcaa atagtagcca tgcttcaaag
     2521 taaaataaaa aaagccaccc cgacgaaact gtcagggcgg ccgaatatac ttgccatgaa
     2581 aaattatgcc tgttctacca gtccggcttt tactgcatac atcactaggc cggccataga
     2641 tttggtgccg gttttggttt tgagcttatc ccggatggcc tccacggtac ggggactgag
     2701 atctacgatg tcggcaattt cttttgtact cttctcatca cacatcaatt tcagaatggt
     2761 gatctcttta tcggtcagtt gcacatcctg cggcattgaa gcttcagcag tacgtttagt
     2821 acgaagaccg ctcagcaatg ctttattggt aaggtcgttg aaatggaaat cattctcata
     2881 cacaccacgg atggcttcat aaatcaggtc agcgcctgac tctttagtaa tataggagtt
     2941 ggcacccatt tccatcattt tggaaataac agaatgatca ttgaggaatg aaagcatgat
     3001 cactttcaca tcgggatata attttttgat ctcaggtaaa gcagtcaatc catccataat
     3061 aggcatctgg atgtcaagta aaattacatc tggtttaatg tggcgcaaca ggttcagcaa
     3121 ttgcataccg ttttcggcct cagccaccat ttgaatgtct ttacgtacct ggagagcagt
     3181 cttgatacca gtgcggaaaa gggcatgatc atcagcgatc gccactttaa taacagcgtt
     3241 agtgtcggga tttttcataa ttggattatt aaaaatgtta ttgaatctaa gtaataatta
     3301 cagactgcaa gttggaaaat tcctgcaata tgtactatag ggaaagtact tgatttcgta
     3361 tttgtggaaa gtaccgctac tttacggaca tatcgaaact gtactatgtg cttataagag
     3421 tttttactag ctccgcagta aataccgaat gtttgaaata gggcagttat gctcttgtta
     3481 acatttacat gtggatatac ttttgttgca gaaagttgat tgacacggat gatctgtctt
     3541 taccctctca tccaatgtcc agaaccgtcc agaaagttat ttccaattcc tttgaaaaaa
     3601 ccgacccgtt tttacgggag acacttcaaa ccggccagtc aactttctct ttttgaataa
     3661 tggctccgtt tttgaaatcc aaaaccaatg aaaaatgaat tgaaaagcaa gagccgtaac
     3721 cgttaagccc tgtctctatg aaacacaacg cttaaaaatc tttttctttt cataacagta
     3781 acgtaagccc cggtcctcat aacgccgggg ttattttttg tacatatacc aactgaaaag
     3841 aaataacaac gaattattgc ttttgtgcaa ccagataagc cctttgttca ccaagggtga
     3901 atttttttct gcctggaata ctatacactt cttttaatac aaaccccgca gattggagca
     3961 tgttttccat ttcattaatg gaaaaaatat aatcaatacc ggtcttggtc tctgttgttc
     4021 cgtccgggtg tataatggta ctttcagctt ccagccgggc aggatgaaaa agtaattttg
     4081 catcggtcag gaacttgacc ccgtttatct cactccagga tttggtaacg gaattcttaa
     4141 atactatttc tgccagcgac cagctgttga tcagcaagta tccgccgggc tttaaaaagc
     4201 tggctgcctt ttttaaaatg gatactgtat cagccgcatt gaagaaattc agactgtttc
     4261 ccatacagat caccatgtca aattgttctt cc
//
LOCUS       DAORLS010000259         4264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11283_length_4264_cov_1.932763, whole genome shotgun sequence.
ACCESSION   DAORLS010000259 DAORLS010000000
VERSION     DAORLS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11283_length_4264_cov_1.932763"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>236
                     /locus_tag="PK977_15755"
     CDS             <1..>236
                     /locus_tag="PK977_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19630.1"
                     /translation="LDNYVLKPLFSFAGQGVIIDVHKEDIEKVKDPENWILQRKVKYA
                     DVIETPDVPAKAEIRIFYFWPPEAERPVATNNLA"
     assembly_gap    237..356
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            364..663
                     /locus_tag="PK977_15760"
     CDS             364..663
                     /locus_tag="PK977_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF19631.1"
                     /translation="MQPKELAPGLNGYYAHGSNMTLGLVEIKAGSNLPMHQHPHEQIT
                     YILEGQLDMTIDGKECKLTAGMYYVIPSNVIHGAVAVTDCKVIDVFNPVREDYKS"
     gene            complement(651..1211)
                     /locus_tag="PK977_15765"
     CDS             complement(651..1211)
                     /locus_tag="PK977_15765"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF19632.1"
                     /translation="MNLAAFNPFPEMSTERLLLRQLRFADAHSLAELRSNKTVNEHLD
                     RPATMQFEEANNFIEKVNRRINDRELLYWVIALKGTGNFAGAICLWNIEADKDRAELG
                     YELHPDYQGQGIMQEALQQVIQFGFDTMQLDLILALTKPANKASEKLLVKNSFLLDED
                     YRYMSRENAKDYLVYYRTSGNRNYDL"
     gene            complement(1249..3507)
                     /locus_tag="PK977_15770"
     CDS             complement(1249..3507)
                     /locus_tag="PK977_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRF19633.1"
                     /translation="MELKYSQHIAALLNLSLKQINSIYELHNEGSTIPFIARYRKEAT
                     GNLDEVAITNVIEQIRYFNDLEKRKETVLKTIEEAGKMTPELKKRIEDCVNATELEDI
                     YLPYKPKRKTKASVAIEKGLEPLAKLLFEQGNDNPEEAASKFINENVKDTAEALQGAR
                     DIMAEWIAENEQARNLVRKLFTEEAVVSSRVLTTKKEEEEAQKYRDYFEFKEALAQSP
                     SHRILAIRRGEKEGFLIMTINIDKERAHAELKRVLLKSSTLAAKQVELAIEDSYNRLL
                     QPSIETEFRMASKTKADEEAIRVFAENLRQLLLASPLGHKRVMAIDPGFRTGCKVVCL
                     DEQGTFLKYNTIFPHAPQNDWNGAVQIIKELVAKYDIEAIGYGNGTASKETEQLVRGI
                     DFGKPVSIFMVNESGASIYSASEVAREEFPNEDVTVRGAISIGRRLLDPLSELVKIDA
                     KSIGVGQYQHDVNQNRLKDALDQVVESCVNYVGVDVNTASKHLLMYVSGLTATVAKNI
                     VEYRTKNGGFKCREELKKVSMLGPKAFEQCAGFLRIPNAANPLDNSAVHPESYHVVEA
                     MAAKTGSNVPELIKNPEIRKQIKAKDFVSETIGQYTIEDILKELEKPGRDPRSPIEEF
                     RFAEGVSSMEDLKPGMKIPGIVTNITNFGAFVDVGVKQDGLVHISHLANRYISDPNEA
                     VKLGQKVMATVLEVDIARKRIALSLKEAGGKTEVREQKSAGSGKKPATKKTEPMNPFQ
                     AKLMELKKNFKD"
     gene            complement(3631..>4264)
                     /locus_tag="PK977_15775"
     CDS             complement(3631..>4264)
                     /locus_tag="PK977_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF19634.1"
                     /translation="KLQLININPGKLTENNISRSVPVYSPINGFVSKVNVNIGKYVNP
                     SDVLFELINPDDMHAALTVFEKDIAKVKPKQQVLVTFVDEPGTDYACEVLLVTKNVDE
                     NRSALVHCHFEKQPERLLPGMFLNARIKVSNAEALAVPEEAVVRFGNTQYVLEQTGKN
                     TFSLVQVETGISENGMVEINSKMLDLSGKTIITKNPYPVLSAMKNTAEEE"
BASE COUNT         1053 a          984 c          791 g         1316 t
ORIGIN      
        1 cctggacaat tatgttttaa agccattatt ctcttttgcc ggccagggtg tgatcattga
       61 tgtgcataaa gaggatattg aaaaggtgaa agatcctgag aattggatat tacaacggaa
      121 agtaaaatat gcggatgtga tagaaacgcc agatgtaccc gccaaagcgg aaatcagaat
      181 tttttatttc tggccacccg aagcagaaag acctgttgcc actaataacc tggcccnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaaa
      361 gacatacaac caaaagaatt agcacccggt cttaatggct attatgctca tggcagcaat
      421 atgacactgg gtttggtaga aataaaagca gggagcaacc tgcctatgca ccagcatccg
      481 catgaacaga tcacttatat actcgaaggg caattagaca tgaccattga cggaaaagaa
      541 tgtaaactca ctgcaggcat gtactatgtg attccttcaa atgtaattca cggagccgtg
      601 gcggtgactg attgcaaagt gattgatgtg tttaaccccg tgagagagga ctataagtca
      661 taattccgat tgccggacgt acgataatat accaggtagt cttttgcatt ttcccggctc
      721 atatacctgt agtcttcgtc aagcaggaaa gaattcttta ccagcagttt ttcagaagcc
      781 ttattggctg gtttcgtaag ggcaagtatg aggtccagct gcattgtatc aaatccaaac
      841 tgaataacct gttgcagcgc ttcctgcata atgccctgcc cctggtaatc cgggtgcaat
      901 tcatacccca attctgcccg gtctttatct gcttcaatat tccagaggca aatagcccct
      961 gcaaaatttc cggtgccttt taaagcaatt acccaataga gcaattctct gtcgttaata
     1021 cgccggttaa ctttttcaat aaaattatta gcctcttcaa actgcattgt tgcaggacga
     1081 tcgaggtgtt catttactgt tttgtttgaa cgcagctccg ccagcgaatg tgcatcagca
     1141 aaacgaagct gcctcagcag caaccgttct gttgacattt caggaaaagg attaaatgca
     1201 gctaaattca tctgcaaaat ctgttgttat aaatatagct ggtattgatc aatctttgaa
     1261 gttcttcttc agttccatca gtttagcctg gaagggattc atcggttccg ttttttttgt
     1321 agccggtttc ttaccactac cggcagactt ctgctcccgt acttccgtct tccctcctgc
     1381 ttctttcaaa gacagtgcaa ttcttttcct tgctatatcc acttccaata cggtagccat
     1441 cactttttgc cccaatttca ctgcttcatt cggatcgctg atatagcgat tggccaaatg
     1501 tgaaatatgt accaggccat cctgctttac acccacatct acaaatgctc caaagttggt
     1561 gatattggtt acgataccgg gtatcttcat ccccggcttc aggtcttcca tactgcttac
     1621 tccttccgca aagcgaaatt cttcaatcgg cgaacgggga tctcttcccg gcttttcgag
     1681 ttctttcaga atatcttcta tcgtatactg accgatcgtt tctgacacaa aatcttttgc
     1741 tttgatctgc ttccttattt ccgggttctt tatcagctcc ggtacattgc tgccggtttt
     1801 agccgccata gcttctacca catggtaact ctcgggatgc accgctgaat tatccaacgg
     1861 gttggccgca ttgggaattc gcaggaaccc ggcacattgt tcaaatgcct ttggcccgag
     1921 catagaaacc tttttcagct cctccctaca tttaaaccct ccgttctttg tacggtattc
     1981 aacaatattc tttgccactg ttgccgtaag gcccgataca tacatgagca gatgtttgct
     2041 ggctgtattt acatctacac caacatagtt tacacagctt tctactacct ggtccaaagc
     2101 atcttttaaa cggttttggt ttacatcatg ctggtactgc cctactccga tactctttgc
     2161 atcaatcttt accagttcac tcagcggatc aagcaatctt ctaccgatac tgatggcgcc
     2221 acgtaccgtt acatcttcgt tgggaaattc ttctcttgct acttcactgg ctgaatagat
     2281 agaagcgccg ctctcattca ccatgaaaat ggaaacaggt ttaccaaaat caattcccct
     2341 gaccagttgt tctgtttcct tactcgccgt tccgttacca taaccgatag cttcgatatc
     2401 atatttggcc accagttctt tgataatctg tacagctccg ttccagtcgt tctgcggtgc
     2461 atgcggaaat atggtgttgt attttaagaa agtgccctgt tcatccagac aaacaacttt
     2521 acacccggta cgaaaaccgg gatcaatggc cataacccgt ttatgtccta atggtgatgc
     2581 cagtaatagc tggcgaagat tctctgcaaa taccctgata gcttcttcat ccgcttttgt
     2641 tttactggcc atacgaaatt ctgtctcgat agaaggctgc aataaacgat tatatgaatc
     2701 ttctatagcc agttctacct gtttggctgc cagtgtggat gattttagta aaactctttt
     2761 caattcagca tgagctcttt ctttatcaat attgatcgtc atgatcagga aaccttcctt
     2821 ctcaccccgg cgtatggcca atatcctatg tgaaggactt tgtgccagtg cctctttgaa
     2881 ctcaaaatag tccctgtatt tctgcgcttc ttcctcttct ttctttgttg tcaataccct
     2941 tgaggaaaca actgcttctt ccgtaaataa tttccgaacc aggttccttg cctgctcatt
     3001 ctctgcgatc cattcggcca tgatatccct ggcgccctgt aaggcttctg cggtatcctt
     3061 tacgttttca ttgataaatt ttgaagctgc ttcttccgga ttatcattac cctgttcaaa
     3121 taataattta gccagtggtt ccaatccctt ttcaatggcg acgcttgctt ttgttttgcg
     3181 tttgggttta tagggcaggt aaatgtcctc cagctctgtt gcattcacac aatcttcaat
     3241 tcttttcttt agctccggtg tcatcttacc ggcctcttca atggtcttga gaacggtttc
     3301 ctttctcttt tccagatcgt tgaagtacct gatctgttct atgacatttg taatagccac
     3361 ttcatccagg tttccggtgg cctctttccg gtaacgggct ataaagggaa ttgtagaacc
     3421 ctcgttatgc agttcatata tactgtttat ttgctttaaa gacaagttta ataaagcggc
     3481 gatatgctgt gaatatttta gttccatagg tcctttgttt gggggagcaa atctaacggt
     3541 agaagcagaa gaaaaaaatt tgtttttacc cacataccag tataaaaaaa gctgtcataa
     3601 aagacagctt ttaactaata agtcaaacaa ctattcttct tcagcggtat tcttcatagc
     3661 tgacaaaacc gggtatgggt ttttagttat gatggtcttt cccgacagat caagcatttt
     3721 actgttaatc tctaccatac cgttctcact gattccggtt tctacctgta ccagactgaa
     3781 tgtgttctta ccagtctgtt cgagtacata ttgtgtattg ccaaagcgta caaccgcttc
     3841 ttccggtacg gccaaagctt ctgcattact cactttgatc ctcgcattga gaaacatacc
     3901 aggcaacaat ctttccggct gtttttcaaa atgacaatgc accagtgcac tccggttctc
     3961 atctacattc tttgttacca gcaatacttc acaggcatag tcagtgcccg gttcatcaac
     4021 aaaggtaacc agcacctgtt gtttaggctt tactttggca atgtcttttt caaagacagt
     4081 cagtgctgcg tgcatatcat ccgggttgat aagttcaaac agcacatcgg atgggtttac
     4141 atatttaccg atgttcacat ttactttaga cacaaatcca ttgatcggtg aatagacagg
     4201 gacactgcgt gaaatattat tctcagtcag tttgccagga ttaatattta tcagttgtaa
     4261 tttt
//
LOCUS       DAORLS010000260         4262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11287_length_4262_cov_1.627050, whole genome shotgun sequence.
ACCESSION   DAORLS010000260 DAORLS010000000
VERSION     DAORLS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_4262_cov_1.627050"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>290)
                     /locus_tag="PK977_15780"
     CDS             complement(<1..>290)
                     /locus_tag="PK977_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19635.1"
                     /translation="IAALVGGVSNGVAYVNNGGSFFNGFWRGALASGLSATFSGAIAG
                     AMPGLGGSILAGSFVGFSGGGFSTLLNGGGGNSAMLQGLLTGGVGGGVGAYFG"
     assembly_gap    291..411
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<412..>922)
                     /locus_tag="PK977_15785"
     CDS             complement(<412..>922)
                     /locus_tag="PK977_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19636.1"
                     /translation="TYTSFQKAASITDGTVQTNFTYGPDYERIKTVMYVNGSIHTTKY
                     FLGDMERINSSYIRDVHYIPVGNSIAIIEKPLYGNSTLHFIYTDYLGSLLTKTDISGI
                     VTAEQNFDAWGRLRDPNNWQYASYITFNFYDRGFTGHEHLPYHYLINMNGRMYDPIEG
                     RMLSPDNYVG"
     assembly_gap    923..933
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<934..>1956)
                     /locus_tag="PK977_15790"
     CDS             complement(<934..>1956)
                     /locus_tag="PK977_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19637.1"
                     /translation="ATGKVIKATDYGGDLEYTYDSRGNQVQVKHGTTILITSIYDQYG
                     RQTSMTDKNAGTSIYSYDAYGQLTQQTDASNNTYTMGYDGFGRMINRQNGVTGELTTW
                     EYYTGGGLTKCKGHQIKKITFSNGVLKEYVYDGLRRPQSETVTVDGIQYVTSFTYDGF
                     SNLASATYPSGVVINNVYDNNGSLTSVSGGDSWNPVTLFTVNSENGYGQLTSYTLGNG
                     KTSQNTYQYGVPTRFYTPGVQDLNFNFDLTRGNLLSRTDALKSITETFQYDNLDRLTT
                     TSVNGVQQLSMNFDGTSSFSRGNIISKTDAGNYVYSNNRIHAVNYITNPAGPTAPPIT
                     LPTMQQ"
     assembly_gap    1957..1972
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1973..>3960)
                     /locus_tag="PK977_15795"
     CDS             complement(<1973..>3960)
                     /locus_tag="PK977_15795"
                     /inference="COORDINATES: protein motif:HMM:NF023677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HRF19638.1"
                     /translation="VAIERIPLSTGVNFISSIKYTTPNIIDDDNIFLGDFNADKKADL
                     LWNYRFKGQNYWAIEYSNGVAFDPASFSFAQNVNISGNNILDKIVVGDFNGDGKSDIA
                     HGYNNTSSTSKISVYYSKGRYSANPFYYEQYDNNLIMPPADVVTGDFNGDGRNDIITR
                     FPNQAQFISIKPNGQEKLLKKVTDGHNVTTSFEYKYLTDKSSFPYVYNRTISLSDPQN
                     QSPFNYVQLPLPVVSSVIVPDGIGGINTTNFTYEDAVLHRHAKGFLGFKKITAVNSVT
                     GITSITESNINTQFAIAYPVKQTTKLTATNEILSENSITTSFVNMSTSTQDIRYFQKI
                     DNTLSKDQLNGTATERINTYDAYGNITTNVTNTGYWSGTSVLVDETTTSNTTFGIHNT
                     PFPAKPVNTTVTNTRAGMPAQSVSTSFTYLSNGLPSSKTDFAGQPLAVTATYSYNSFG
                     NVTQTVVSATGVSNRISNVTYDTKGRYPTIKESWDGTVVQREMLTYDGKWGKPLTQIS
                     TECLTTAYTYDPYGRLKQTTFPEGYTSTNSLNWYVLWNPLQPYYILSDYSGGKPDSKI
                     WMDIFNRGVKTETSGFNNQWLTELTTYNSRGLQATKTNSYYPTETPVVTTSSYDVYGR
                     LQQASNPLGAINYTYTKLTGGKLQVTTQNSAGQSSSKVS"
     assembly_gap    3961..4016
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4017..>4262)
                     /locus_tag="PK977_15800"
     CDS             complement(<4017..>4262)
                     /locus_tag="PK977_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19639.1"
                     /translation="HYFYAKPSQNVLLPGDFDGDGNQDYILIVAARPQSNQEIFKAFI
                     SLPAKNEFNMEISGFGAGPDSYPGSYARTIAEAKRAIP"
BASE COUNT         1099 a          838 c          822 g         1299 t
ORIGIN      
        1 ccgaaataag ctccaactcc accaccaact cctcctgtca aaagcccttg aagcattgct
       61 gagttgccgc cgccgccatt taataatgtg ctaaacccgc cgcctgaaaa cccgacaaaa
      121 gaaccagcta aaatacttcc ccctaaaccc ggcattgctc cagctattgc tccactaaaa
      181 gttgcagaaa gtccacttgc caaagctccc ctccaaaatc cattaaaaaa actaccccca
      241 ttatttacat acgccactcc attgctaacc cctccaacta gtgctgcaat nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacctacat
      421 agttatcggg actgagcata cgaccttcaa tagggtcata catacggcca ttcatattga
      481 tcaggtagtg gtaaggcagg tgctcatgcc cggtaaaccc tcggtcataa aagttgaacg
      541 taatataact ggcatactgc cagttattag ggtcacgcag cctgccccag gcatcaaagt
      601 tttgttctgc tgtaactatt ccacttatat cggtttttgt aagcaggctt ccaagataat
      661 cagtgtagat aaaatgtaaa gtactgttgc cgtataaagg tttttcaatt atagcaatac
      721 tgttacctac gggtatataa tggacatccc ggatatagct actgtttatc ctttccatat
      781 ctcctaagaa atacttggtg gtatgaatgc ttccgtttac atacatcacc gtttttatac
      841 gctcgtagtc ggggccataa gtaaaatttg tctgtacagt cccgtctgtt atagatgccg
      901 ccttctggaa tgaggtatat gtnnnnnnnn nnngctgctg catagtgggc agtgttatgg
      961 gtggagctgt cggccctgcc gggttagtaa tataattaac agcatgtatc ctgttattgc
     1021 tgtacacata attacctgca tcagttttac tgattatatt gccacgggag aaggaggatg
     1081 tgccatcaaa gttcatactt agctgctgca ctccatttac tgaggtggta gtcagcctgt
     1141 ctaagttatc atactgaaat gtttccgtaa tactttttag tgcgtctgtc cggctgagca
     1201 ggttgcctcg tgtaaggtca aaattaaaat tcaggtcctg cacaccgggg gtgtaaaatc
     1261 ttgtcggaac tccgtactga taagtgttct gagatgtttt tccattgccc aaagtatagg
     1321 atgttaactg gccataacca ttttcggagt tgaccgtaaa caaagtcacc gggttccagc
     1381 tatctccgcc ggatacagag gtaaggctcc cattgttatc atacacatta ttaataacaa
     1441 cacctgatgg atatgtagcg ctggccaagt tgctgaaacc atcataagta aagctggtaa
     1501 catactgtat gccatcaacg gtaacagttt ctgattgagg gcggcgcagg ccatcataaa
     1561 catattcttt gagtaccccg tttgaaaatg taattttctt aatctggtgg cctttgcatt
     1621 tagtaaggcc accgcctgta taatactccc aagttgtgag ttcacctgta acgccatttt
     1681 gccggtttat catacgacca aaaccgtcat atcccattgt gtaagtgtta ttggaagcat
     1741 ccgtttgctg ggtaagctgc ccgtatgcat cataactata aattgaagtt ccggcgtttt
     1801 tatcagtcat actggtttgt cttccgtatt gatcatatat gctggtaata aggatggtag
     1861 ttccatgctt cacctgcacc tggttgcccc ggctgtcata agtatattca aggtcacctc
     1921 catagtctgt tgctttaatt actttgccag tagcatnnnn nnnnnnnnnn nngaaacctt
     1981 agaactgctt tgtccagcgc tattttgagt tgttacctgt agtttcccac cagtaagttt
     2041 tgtataagtg taattgattg ctccaagagg gttggaagct tgctgtagtc tgccataaac
     2101 atcgtatgaa cttgttgtaa caacgggtgt ttcagtaggg taataagagt ttgtttttgt
     2161 tgcctgtaat cctcttgagt tatatgttgt aagttctgtc agccattggt tgttaaatcc
     2221 tgaggtctct gtctttacgc ctctattaaa aatatccatc catattttac tgtcaggttt
     2281 tccgcctgaa tagtcagaga gtatgtaata tggctgtaac ggattccaaa gaacatacca
     2341 gttcaaagaa tttgttgaag tgtacccttc ggggaaagtt gtttgtttca accggccata
     2401 aggatcatat gtgtaagccg ttgtcaagca ttcggtagaa atttgagtca atggcttacc
     2461 ccatttgcca tcatatgtga gcatttcccg ttgaacaact gtgccatccc aagattcttt
     2521 tatagtagga tatctgccct ttgtgtcata ggtaacattg gatattctgt tgctaacacc
     2581 tgtggctgaa accacagtct gtgttacatt cccaaaacta ttgtatgagt aagttgctgt
     2641 aacagccagc ggctgtcctg caaagtctgt ttttgaagag ggtaacccat tactaaggta
     2701 ggtaaagctt gtgcttaccg attgtgctgg cattcctgcc cgggtattgg taacagtagt
     2761 attaactggt ttggctggga atggggtatt atgaataccg aaagttgtat ttgaggtggt
     2821 ggtttcatca acaagaacag atgttccgct ccagtaaccg gtgttagtta cattcgttgt
     2881 aatatttcca taggcatcat aggtattaat tctctctgtg gctgttccgt tcaattgatc
     2941 cttacttaaa gtgttgtcaa ttttttgaaa gtatcgtata tcctgcgtgc tggttgacat
     3001 attaacaaag gatgtagtaa tagagttttc agaaagaatt tcgtttgtcg cagtgagttt
     3061 tgtggtttgt ttgacaggat atgctatagc aaactgagta ttaatattgc tttcggtgat
     3121 tgatgtaatt cctgtcactg agttcacagc agttattttt ttaaacccaa gaaatccttt
     3181 agcatgcctg tgcaaaactg catcctcgta tgtaaaattt gtagtattta tcccccctat
     3241 accatctggc acaataaccg acgaaaccac tggtaacggc aactgtacat agttaaaagg
     3301 agattggttt tgggggtcgc ttaaagaaat agtcctgtta taaacatatg gaaaacttga
     3361 cttgtcagtc aagtatttat attcaaaaga agtggttaca ttatgcccat cagttacttt
     3421 tttgagtaat ttttcctggc catttggttt aatacttata aactgagcct ggtttggaaa
     3481 tcttgtaata atgtcatttc ttccatcacc attgaaatca ccggttacaa catctgccgg
     3541 aggcattatt agattattgt catattgctc ataataaaac ggatttgctg aatatcgtcc
     3601 cttgctataa taaacagaga ttttggatgt tgaacttgta ttgttatagc catgtgcaat
     3661 atcgcttttg ccatcaccgt taaaatcacc aactactatt ttatcaagaa tattattgcc
     3721 actgatattg acgttttgag caaaggaaaa tgatgcagga tcaaatgcaa ccccattact
     3781 gtattctatt gcccagtaat tttgtccctt aaacctgtaa ttccataaaa gatcagcttt
     3841 cttatctgcg ttaaaatcac caaggaatat attatcatca tcgatgatgt tgggcgtggt
     3901 atatttaata gacgatatga aatttacccc tgtagatagt ggaatccttt ctatagcaac
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggg
     4021 attgcccttt ttgcttcagc aatagtcctt gcgtaagagc ccgggtaact atccggtccg
     4081 gctccaaaac cggatatttc catattgaac tcatttttag caggcaggct gataaaagcc
     4141 ttaaatattt cctgattact ttgaggtctt gccgcaacta tcaatatata gtcctggtta
     4201 ccgtcaccgt caaagtcgcc gggtaacagt acattttgag acggctttgc ataaaagtag
     4261 tg
//
LOCUS       DAORLS010000261         4261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11291_length_4261_cov_2.138136, whole genome shotgun sequence.
ACCESSION   DAORLS010000261 DAORLS010000000
VERSION     DAORLS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11291_length_4261_cov_2.138136"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK977_15805"
     CDS             <1..793
                     /locus_tag="PK977_15805"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRF19640.1"
                     /translation="VIIYNRDNYNLPTDPEKNVAGVVLLSIIEKMGEDIGFSLSVEKH
                     IKPGSGIGSSAASAAGAAVAANQLLGNIFTNEELVQFAMNGEKLASGVKHADNIAPCI
                     YGGVTLIRSIHPLDIVSIPAPDMFVTVVHPQIEIRTADARQILKQQVSLKDAIKQWGN
                     IAGLVTGFMKGDYELIGRSLEDVIIEPVRSILIPGFEELKGKCKEAGALGGGISGSGP
                     SVFMLSKNETIARDVESVMRDIYNRIGIKYNTYVTTINNKGIEIL"
     gene            780..2075
                     /gene="thrC"
                     /locus_tag="PK977_15810"
     CDS             780..2075
                     /gene="thrC"
                     /locus_tag="PK977_15810"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRF19641.1"
                     /translation="MKYYSTNKQSPFVDFKEATIKGQANDKGLYFPQSIPILNQEWLD
                     NIEDYSNEEIAFRVIKPYTGNTIQEEKLYEIVKETVNFPIPLVKVDESAYTLELFHGP
                     TLAFKDVGARFMSRCLGYFVKDRSTKVTVLVATSGDTGGAVANGFYDVDGVEVVILYP
                     SGKVSPVQEKQLTALGKNIQALEVQGTFDDCQAMVKTAFADKDVTDKLFLTSANSINV
                     ARWLPQQFYYFFAWKQWKNKDNPPVISVPSGNFGNICAGILAMESGLPVKHFIAACNI
                     NDIVTDYLQTQQLIPRAALPTLSNAMDVGNPSNFSRILEIFNNQFPDLQKRMSSFTIT
                     DEETRKTIRQVYDNYNYLLDPHGAVGYLSLKRYLADHPAAQGIFLETAHPVKFPDAVE
                     KETGYSIPIPASVRDIMAAEKKSTVIKPNYDLLKDFLLS"
     gene            2140..2706
                     /locus_tag="PK977_15815"
     CDS             2140..2706
                     /locus_tag="PK977_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19642.1"
                     /translation="MQDIEPFYNWRHIYISEEDQRSPFFGRTYSEFEFSQTVYNYYIH
                     PQWDDFGSRTLYLKVLLADYEEKYAVIEMIGEWNDAIENDIMELKREVLEKFMYEGIY
                     KFILIGENVLNFHSDGKDYYEELFEEVTDENGWVVCLNMPEQTQYDFKHAHLNRFIEL
                     MQLDNWRTYKPFHLYKKIDSELTARLGF"
     gene            2814..3485
                     /locus_tag="PK977_15820"
     CDS             2814..3485
                     /locus_tag="PK977_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRF19643.1"
                     /translation="MKWSQLFTSSVGKKWVMALTGIFLILFLIVHVALNATIWANDGG
                     KMFEAGAHFMGSNIVPRILEIGLIAGFLIHIIQGIMLELQNRSKRKRGYAANLGNRGS
                     KWYSRSMGLLGTLILIFLIIHMAHFWVPNRSNQGWLLGPEISLYDEMREVFQQEWVVI
                     VYVLGCFSLAWHLMHGFQSAFRTLGVSNKKYLTLLNSLGIGFSIIVPLVFALMPVSFY
                     FGWIN"
     gene            3512..>4261
                     /locus_tag="PK977_15825"
     CDS             3512..>4261
                     /locus_tag="PK977_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF19644.1"
                     /translation="MLDSKIPAGPLENKWTDYKGHCKLVNPANKRKLEVIVVGTGLAG
                     ASAAASLGELGYKVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVFRLFYDTVKG
                     GDYRAREANVHRLAEVSVNIIDQCVAQGVPFAREYGGLLNNRSFGGTQVKRTFYAAGQ
                     TGQQLLIGAYQALERQVALGNVQMYSRHEMLDVVIIDGKARGIICRNLVTGQLERFFG
                     HAVLLCTGGYGNVFYLSTNAMGSNVTAAWKAH"
BASE COUNT         1244 a          765 c          976 g         1276 t
ORIGIN      
        1 ggtaataatt tataacaggg ataattataa tttacccact gatccggaga agaatgttgc
       61 cggtgttgtt ttactttcta tcattgaaaa aatgggagaa gatattggct ttagtctttc
      121 tgttgaaaaa catataaagc caggtagcgg tattggttcc agtgctgcaa gtgctgctgg
      181 tgctgcagtg gccgccaatc aattgctcgg gaatattttt acgaatgaag agttagtgca
      241 gtttgccatg aatggcgaga aattggcctc cggagtcaaa catgctgata atattgctcc
      301 ctgtatttat ggaggagtta ccctgatacg ttccattcac ccgctggata ttgtttccat
      361 tccggcgcct gatatgtttg taacagtagt acatccacaa attgaaatac gcacagcaga
      421 tgcaagacaa attctaaaac agcaggtgtc acttaaagat gcaataaaac aatggggaaa
      481 tattgcaggc cttgtcacag gttttatgaa aggggattat gaattgatag gccggtcatt
      541 ggaggatgtt atcatcgagc cggtccgcag tattctcata cccggttttg aagagttaaa
      601 gggcaaatgc aaagaggccg gtgctttggg aggcggaatt tctggttctg gcccatccgt
      661 ttttatgctc agtaaaaatg aaacaatagc ccgggatgtt gaatcagtca tgagagatat
      721 ctataaccgg attgggatta agtacaatac ttatgtaaca actattaata ataagggcat
      781 tgaaatatta tagcaccaat aagcaatctc ctttcgtaga ttttaaagaa gcgaccataa
      841 agggacaggc taatgacaag ggattgtatt ttcctcagtc aattcctatc ttaaatcaag
      901 aatggttaga caatatagaa gattattcta atgaagagat tgccttccgt gtaataaaac
      961 catacaccgg gaatacaatt caggaagaaa aattgtacga gattgtaaaa gaaacagtca
     1021 atttcccgat tccgctcgtt aaagttgacg agtctgctta tactcttgaa ctatttcatg
     1081 ggccaacgct ggcgtttaag gatgtaggtg cccgttttat gagccgatgt cttgggtatt
     1141 ttgtaaaaga tagatcaacg aaggttacag tgcttgtggc tacttccggt gacaccggtg
     1201 gtgcggtggc caacggattt tacgatgtag atggggtaga agtggtgatt ctctatccat
     1261 caggaaaggt aagccctgtc caggaaaaac aattgacagc gttaggtaag aatattcaag
     1321 cactggaagt tcagggaaca tttgatgatt gccaggccat ggtgaaaacg gcttttgccg
     1381 ataaagatgt gacggataaa ctctttctta cttctgccaa ctccattaat gttgcccgct
     1441 ggctgccaca gcaattttat tacttctttg cctggaagca atggaagaat aaagacaatc
     1501 caccggtgat atcagtcccg agcggcaatt ttggaaatat ctgtgccggt attcttgcca
     1561 tggaatcggg attacctgtt aagcacttta ttgctgcctg taatattaat gatatcgtta
     1621 ctgattacct gcaaacacaa caattgatac ccagggctgc tttacctaca ttaagtaatg
     1681 ctatggatgt ggggaatccc agcaattttt ccaggatatt ggagattttt aacaaccagt
     1741 ttcctgattt gcagaagcga atgtcttctt ttacgatcac agatgaagaa acccggaaaa
     1801 caatcaggca agtgtatgat aattacaatt atttgctcga tccgcatggg gcagtcggtt
     1861 atctatcttt aaaacgttat ctcgctgatc atcccgcagc gcagggaata tttctggaaa
     1921 cagcacatcc tgtcaagttt cctgatgctg ttgaaaaaga aacagggtat tctattccca
     1981 tcccagcatc agtgcgggat atcatggctg ccgaaaagaa aagcactgtt ataaaaccta
     2041 attacgattt attaaaagat tttcttttga gttaagtctt tgaaatggtt tgtttcaact
     2101 attcagccat agcttaaatt tgcagggaag ttttgaatca tgcaggatat agaacccttt
     2161 tacaactggc gtcacattta cataagtgaa gaagatcaac ggtccccttt ttttggccgt
     2221 acgtattcag aatttgagtt ttcccagact gtttataatt attatatcca tccgcaatgg
     2281 gatgattttg gcagtcgtac actttacctg aaagttttgc ttgctgatta tgaagaaaaa
     2341 tacgctgtca tcgaaatgat cggggaatgg aatgatgcta tcgaaaatga tataatggaa
     2401 ttaaaaaggg aagtgctgga aaagttcatg tatgagggta tttataaatt tatactgatt
     2461 ggagaaaatg tgctgaactt ccatagtgac ggcaaggatt attatgaaga attattcgag
     2521 gaggtaacag atgaaaatgg ctgggttgtt tgtttaaaca tgccggagca gacacaatat
     2581 gactttaaac atgcccacct gaacaggttt attgaactga tgcaactgga taactggcga
     2641 acctataagc cttttcactt gtataaaaag atagattcgg aactcacagc caggctggga
     2701 ttctgatgat ttaagtttat taattaattg actatctaaa tcttttgttc aatattgacc
     2761 gtagcacagt atttttgcgg caaatttgaa ttactcctct aagtacgaat catatgaaat
     2821 ggtcccaact ctttacttct tctgtcggca aaaaatgggt tatggcgctg acaggtattt
     2881 tcctcattct ttttttaata gtccatgtgg cgcttaatgc caccatttgg gctaatgatg
     2941 gtggtaaaat gtttgaagcc ggggcccatt ttatgggttc taatattgtt ccccgtattc
     3001 ttgaaattgg tctaattgcc ggctttttaa tacacatcat ccagggaata atgcttgaac
     3061 tgcaaaaccg cagtaaaaga aagagaggtt atgctgcaaa cctgggtaac aggggtagta
     3121 aatggtatag ccgcagtatg ggcctgttgg gcaccctgat cctgatcttc cttattattc
     3181 acatggctca tttctgggtt cctaaccgta gtaaccaggg ctggctgctt gggcccgaga
     3241 taagccttta cgatgagatg agagaagtat tccagcaaga gtgggtagtt atcgtttatg
     3301 tactgggttg tttttcgctt gcctggcatt tgatgcatgg cttccaaagt gcttttcgta
     3361 cactgggagt gagcaataaa aaatacctga cattattaaa ttcattgggc attggattct
     3421 ccattattgt cccacttgtt tttgcattaa tgccggtaag tttttatttc ggctggataa
     3481 attaatagct aactaaaagt aacctgagtt tatgttggat tcaaaaattc ctgcaggacc
     3541 gctagaaaat aaatggacag attataaagg tcattgcaag ctggtaaacc cggccaataa
     3601 acgcaaactg gaagttatcg tggtaggtac cggtcttgcc ggtgcttctg ctgcagcttc
     3661 tttaggcgaa ttaggctata aagtaaaagc tttttgtttc caggattctc cccgccgtgc
     3721 acactccatc gctgcgcagg gtggtatcaa tgcagccaag aactaccaaa atgacggcga
     3781 ttctgttttc cgtctttttt atgatactgt gaagggaggt gattaccggg caagagaagc
     3841 caatgtacac cgattggcag aagtaagtgt aaacattatt gaccagtgtg tggcgcaggg
     3901 tgtgcctttt gcaagagagt atggaggttt attgaataac cgttcttttg gaggcaccca
     3961 agtaaaaaga actttttatg ctgccggtca aacaggtcag cagttattga ttggtgctta
     4021 ccaggcatta gaaaggcagg tggctttagg caatgtacaa atgtacagcc gtcatgaaat
     4081 gctggatgta gtgatcattg atggtaaagc aagaggaatt atttgccgta acctggtaac
     4141 cgggcagtta gaaagattct ttggacatgc agtattgtta tgtaccggtg gatatggtaa
     4201 tgtattttat ctttctacca atgcaatggg cagcaatgtt actgctgcat ggaaagcaca
     4261 t
//
LOCUS       DAORLS010000262         4234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11388_length_4234_cov_1.414214, whole genome shotgun sequence.
ACCESSION   DAORLS010000262 DAORLS010000000
VERSION     DAORLS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11388_length_4234_cov_1.414214"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PK977_15830"
     CDS             complement(<1..338)
                     /locus_tag="PK977_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19645.1"
                     /translation="MKKIILPLLLLISCTAFSQSLKKYPVSKSGCSYYNYCDAKFDAS
                     KSQDSSDIYTGECAVSDVTYGIICVKLLNTVADLQMAEDLLIAYMDFLKQSFEITSTA
                     GYGKGHRLNN"
     gene            complement(357..>726)
                     /locus_tag="PK977_15835"
     CDS             complement(357..>726)
                     /locus_tag="PK977_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19646.1"
                     /translation="STVYADDCEKDGVTYGIYCIKLKKPITDLKAAEDTMITYLDFLK
                     LDYGIVKGKGYDKGHKLKKDDNTRGIYDTWEDVDKNKWKVKAWTNGNFICVLHVHGTK
                     ELPEKKTEIFLEGLRFPGMK"
     assembly_gap    727..862
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1032..>2136
                     /locus_tag="PK977_15840"
     CDS             1032..>2136
                     /locus_tag="PK977_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HRF19647.1"
                     /translation="MSANFNYGIDRLTTAVALQIASGKIKGVLNKAAVEKVNSSHKAV
                     QEIVAANKTVYGVNTGFGILANTAISADDTATLQYKILQSHSVGVGDPIPQEVAKLML
                     ITKVQALAQGYSGVQLATLQRIIWHVDNDVIPVVPEKGSVGASGDLAPLAHLFLPLIG
                     LGEVYYKGEKRDTSSVLRDAGLQPIQLGPKEGLALINGTQFILSFAVKAVQRMHNCLE
                     AADIIGAMSLEALTGTKAPFDERLHELRPYNGNKLVAQRLRLLLNDSEIMQSHVDCGR
                     VQDPYSLRCMPQVHGASRNAWLHLKELTEIELNAVTDNPIILSADDTISGGNFHGQPM
                     ALPLDYACFAAAEVGNMADRRCYLLLEGKWGLPV"
     assembly_gap    2137..2285
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2286..2608
                     /locus_tag="PK977_15845"
     CDS             <2286..2608
                     /locus_tag="PK977_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HRF19648.1"
                     /translation="VSMGSISGRKLNKVLDNLEFILAIELLSACQAIEFRRPLKSSAL
                     LEYAHDYVREFVGFASEDRIFADDINQVAGLIKDFSFVEKLNGFAASKGISLNSGFDG
                     FNGL"
     assembly_gap    2810..2964
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2965..>3188)
                     /locus_tag="PK977_15850"
     CDS             complement(<2965..>3188)
                     /locus_tag="PK977_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19649.1"
                     /translation="IIRIGTCGAYTTELSLFDLVNVNYAASESTFAKYAWGIGGNSIK
                     HQGKAFSVIRRTAKRLNQEIKNCNVHSSDI"
     assembly_gap    3189..3208
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3209..3441)
                     /locus_tag="PK977_15855"
     CDS             complement(<3209..3441)
                     /locus_tag="PK977_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19650.1"
                     /translation="MSIHISAKKGEIAKTVLMPGDPLRAKYIADRFLQDVELVSQVRN
                     AFYFTGKYKGKPITIGASGMGCPSIGIYSYELYT"
     gene            3596..>4234
                     /locus_tag="PK977_15860"
     CDS             3596..>4234
                     /locus_tag="PK977_15860"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218468.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRF19651.1"
                     /translation="MTTITRHYDPVKYKTPTGPTISCKGWIQEAALRMLLNNLNPEVA
                     ERPDDLIVYGGRGKAARNFEALDNIIAALKVLENDESLLIQSGKPVGILKTHKDAPRV
                     LISNSQLVPNWANWKHFDELEQKGLMMYGQMTAGSWIYIGSQGIVQGTYETYAALANK
                     HFGGTLKGTLNVTAGLGGMGGAQPLAITMNEGVALIAEVEEWRIDKRIETKYL"
BASE COUNT         1034 a          813 c          870 g         1057 t
ORIGIN      
        1 ttattgttta accggtgacc tttgccataa ccggcagtgc tggtgatttc gaaactttgt
       61 ttgaggaagt ccatgtaagc aatcagcagg tcttctgcca tttgaagatc ggcaaccgta
      121 ttcagcagct tcacacaaat gataccatag gtaacatcgg ataccgcaca ttccccggtg
      181 tagatatcgg aggaatcctg gctttttgaa gcatcaaatt ttgcatcaca gtaattataa
      241 taagaacaac ccgatttgct taccgggtat ttcttcaggc tttgcgaaaa agcggtacaa
      301 ctgatcaaga gtaataaggg caggatgatc tttttcattt tctaatttgc ttattattat
      361 ttcatgccgg gaaaacgaag cccttccagg aatatttctg tttttttctc aggcagctct
      421 ttggtgccgt gtacatggag tacacagata aaattaccat tggtccatgc tttcactttc
      481 catttattct tgtccacatc ttcccatgta tcatagatgc cacgggtatt atcatctttc
      541 tttagcttat ggcctttatc atagcctttg ccctttacaa tgccatagtc cagtttcagg
      601 aaatcaagat aggtgatcat cgtatcttca gccgctttca ggtcagtgat gggctttttt
      661 agtttaatgc agtagatgcc ataggtcact ccgtcctttt cacaatcatc ggcatataca
      721 gtagaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nncagtaata aagttagatg gcgcatataa tcaattttgt
      901 tggtaatgct tcctatttac cgggcgggat cggtgttgta aagataaact cctttatgta
      961 ttggttgcaa gcagttcgta aataaaacca ctcgtttcaa atctttatct tgcagcccat
     1021 aattgcctgc tatgagtgcc aattttaatt acggaattga ccggctgacg actgctgttg
     1081 ctttacaaat tgcttctgga aaaataaaag gggtgctgaa taaagcagcg gtcgaaaaag
     1141 tgaacagcag ccataaagcc gtacaggaga ttgttgctgc taataaaacg gtttatggtg
     1201 tcaataccgg ttttggcatt ttggccaata cggctatttc tgcggatgac acggccacac
     1261 tgcaatataa aatcttgcaa agtcatagcg taggagtggg tgatcccatt ccgcaggaag
     1321 ttgccaaatt gatgcttatc accaaagtgc aggcactggc acagggttat agtggtgtgc
     1381 agttggctac attacaaaga atcatctggc atgtggataa tgatgtgata ccggtagtgc
     1441 cggagaaagg aagtgtgggg gcttccggag acctggcacc attggcacat ttgtttctcc
     1501 ccttgatagg attgggagaa gtttattata aaggagagaa aagagatact tcatccgtgt
     1561 taagggatgc gggattacaa cccattcagc ttggaccaaa agaaggcctt gcactcatca
     1621 acggcacaca attcatttta tcctttgcgg tgaaagcagt tcagcggatg cataattgcc
     1681 tggaagcggc tgatatcatt ggtgcgatga gcctcgaagc gcttacaggc accaaagctc
     1741 cctttgatga acggctgcat gaactccgcc cttacaatgg aaataaatta gtggcacaac
     1801 gtttacgtct gctgctgaat gattccgaga tcatgcagag ccatgtggat tgcggccggg
     1861 tacaggatcc ttattcactc cgttgtatgc cacaagtgca tggcgcttcc cgcaatgcat
     1921 ggctgcattt aaaagaactg acagagatcg aactgaatgc agtgacagat aacccgatca
     1981 ttctttctgc cgatgatacc atcagtggcg gcaatttcca tggtcagccg atggcattgc
     2041 cattagatta tgcctgcttt gcagcagcag aagtaggtaa tatggctgac cgccgttgtt
     2101 atttgttgct tgaaggcaaa tggggcttgc ctgtatnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnatgta agcatgggca gcatcagcgg caggaaactg aacaaggtgc tggacaattt
     2341 ggaatttatt ttggctatag aattactctc agcctgccag gccattgaat tcagaaggcc
     2401 gttgaagagt tctgcgttat tggaatatgc ccacgactat gtaagggagt ttgttggctt
     2461 tgcttccgaa gaccgcattt tcgccgacga catcaaccag gtagccggcc tcatcaaaga
     2521 tttttctttt gtggagaagc tgaatggttt tgccgcttca aaaggcattt ccctgaatag
     2581 cgggtttgat gggtttaatg gattgtagtg ttttgttatg ccgacgaagg aggagtctca
     2641 gagttttcat ccttacttaa gttaatagtc tttgagatgc ctcgttcctc ggcatgacaa
     2701 gaccctgaag gtttcaatag tctgaattaa atcacaactt caccgcggct tccagcgcca
     2761 gttccatcat gggcaacaat gccttttctc tttcttcagc agagatcttn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnaaaata tcgctggaat gaacattgca gtttttgatt
     3001 tcctggttca gccgtttcgc tgttcgcctg attaccgaaa aagcctttcc ctggtgttta
     3061 atactgttgc cgccaatgcc ccaggcatat ttggcaaaag tggattcgct ggcagcatag
     3121 ttcacattta ctaaatcaaa caggctgagt tcggtagtat aagcgccgca ggtaccgatg
     3181 cggataatnn nnnnnnnnnn nnnnnnnngt gtacaattcg taggaataaa taccgatgga
     3241 agggcagccc ataccactgg cgccgatggt aatcggtttg cctttatact ttcccgtaaa
     3301 ataaaatgca ttgcgtacct ggcttactaa ttctacatct tgcaggaaac ggtcggcaat
     3361 atacttggcc cgcagcggat caccgggcat caatacagtc tttgctattt cgcctttttt
     3421 ggcgctgata tgaatactca ttggtaatgg tttaagtttc ggcggacaag atatattttt
     3481 tatccaagaa taacgacttt tgcaatgatt atgctaaatg ttgaatgctg tattttaaat
     3541 gataccagac tccctacatt caacatttaa aaatcaacat tcaacataat acaaaatgac
     3601 aacgattacc cgccattacg atcccgttaa atacaaaaca ccaaccggcc ctaccatttc
     3661 ctgcaaagga tggatacagg aagccgccct gcggatgtta ttgaacaacc tgaaccccga
     3721 agtagccgaa cgtccggatg atctcatcgt ttacggcggc agaggaaaag cagcccgcaa
     3781 ctttgaagcg ctggataata tcattgcagc gctgaaagtg ttggagaatg atgaatcctt
     3841 gctcattcaa tccggcaagc cggtaggaat tttaaaaaca cacaaggatg caccaagggt
     3901 gctcatcagt aattcgcaat tagttcccaa ctgggccaac tggaaacatt ttgatgaact
     3961 ggaacaaaaa ggcctgatga tgtacggcca gatgacagcc ggctcctgga tatatatcgg
     4021 cagccagggt atagtgcagg gcacttatga aacctatgct gctcttgcca acaaacattt
     4081 tggtggcaca ttaaaaggca cattgaatgt tacagccggt ctcggtggta tgggcggcgc
     4141 acagccactg gccatcacca tgaacgaagg agtggcactg attgcggaag tagaagagtg
     4201 gcgcattgac aaacgcatcg aaacaaaata cctc
//
LOCUS       DAORLS010000263         4211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11478_length_4211_cov_2.177093, whole genome shotgun sequence.
ACCESSION   DAORLS010000263 DAORLS010000000
VERSION     DAORLS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11478_length_4211_cov_2.177093"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..182
                     /locus_tag="PK977_15865"
     tRNA            109..182
                     /locus_tag="PK977_15865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Met,seq:cat)
     gene            238..828
                     /locus_tag="PK977_15870"
     CDS             238..828
                     /locus_tag="PK977_15870"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRF19652.1"
                     /translation="MTTIRLARPTDAAGILAIYSPYIASTSFTFETEVPSEDDFTDRI
                     TSYLANWPWLVCEVDGQIAGYAYASRYRERVAYQWSAECSVYVQDDHHRSGIARALYT
                     ALFSILKLQGYRNVYAVINLPNERSVAFHESMGFRWFATYEQVGYKLGQWKNVGWWRL
                     IINEFEQEPAPVISFSLLDQSFVPQLLNDVTGLLNK"
     gene            complement(889..2115)
                     /locus_tag="PK977_15875"
     CDS             complement(889..2115)
                     /locus_tag="PK977_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRF19653.1"
                     /translation="MLTISQRGQLMPPSPIRKLVPYAEAAKKKGIKVYHLNIGQPDIE
                     TPPAILDAVRNADIKVLEYSHSAGNESYRRKLVQYYKKVGIDISHDQIMITTGGSEAI
                     LFGFFTCMNPGDEVIIPEPFYANYNGFACAAGVHVVPITSKIDNGFALPPIHDFEKVI
                     TDKTKAIIICSPNNPTGYLYSREEMEALKEICIKHNLYLFSDEAYREFCYDGDYVSAM
                     HLQGLEQHVVLMDTISKRYSACGARLGAFVTKNKSVYDAAMKFAQARLSPPGLAQIMG
                     EAAVDLPDSYFDAPKKEYQRRRDLLVSRLNTMQGVFCPNPGGAFYAIAKLPIDDSDKF
                     CQWLLEEFSYNNQTVMLAPATGFYGTAGLGKNEVRLAYVLNLDAINAAMDCLEKALEQ
                     YPGKVVAKEEKAVLSK"
     gene            2293..2556
                     /locus_tag="PK977_15880"
     CDS             2293..2556
                     /locus_tag="PK977_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19654.1"
                     /translation="MKPKPLILFPHAIMLAVLALFCTCTGRDNGLSGYKHEYVTETAK
                     TRINPGLAGFSIYSTFDNSDEIASAEDNKSLMSLLKEYEKILP"
     assembly_gap    2617..2642
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2643..>3954
                     /locus_tag="PK977_15885"
     CDS             <2643..>3954
                     /locus_tag="PK977_15885"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF19655.1"
                     /translation="TLLLGFFAVYIIKQQKQITLKQNREQAKSLAQALAVNARFYIIS
                     HDEEMLLKNLQSINKFPDLEYAAILENDGKVLAHTNPAYLGMKLADSIGLKGIMDSDG
                     SIIFENSKVADVTLPIYDEGGSRLIGWAKLALHRSSISNEFSIIVREVTLYMLLGLLI
                     SIIIAYFIAKNLSKELNILSGLTEKIAHGQVKERAAGFKNIEVERLGKSFNRMLDELN
                     TNQQLMNTIIENLPVGVWLLNEKGEIISSNTEGERIWAGAKYVGSEHFGEYKGWFLNT
                     GKKIEPEEWAAARIMRTGKAVLNEELEIECFDKTRKIIINSAVPLFDEDHKISGGVII
                     NVDITGIREKEKELLLAQKQIDLTDATMKIAFDQSPIGMALVSTEGKLLEANKEFCQI
                     LGYTEEEIAKVSIQDISHPDDMQIDVEYAMKLLRGEVDKYKIEKR"
     assembly_gap    3955..3967
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3968..>4211
                     /locus_tag="PK977_15890"
     CDS             <3968..>4211
                     /locus_tag="PK977_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF19656.1"
                     /translation="RKDKTLVWANLTVHLVRHKSGVPLYFISQLEDITERKAAELKLR
                     ESEEKFRTLIQKLQIGIIKHKADTSIELFNDAALQL"
BASE COUNT         1248 a          808 c          936 g         1180 t
ORIGIN      
        1 gcaaaaaact gatttttcta tgaggacggt tccggataca gcctctccga aaggagaggc
       61 ttttttcttt aaaaaataag ctgaaatcca ttatttttgc cgcccaatgc cggggtagct
      121 cagctggtta gagcatcgga ttcataaccc gaaggtcggc agttcaagtc tgctctccgg
      181 tacaataaag gcccctaaat agggcctttt ttatttcttt tttgctttat ttacaagatg
      241 acaactatcc gactggcccg gcctactgat gccgctggta tattagccat ttattcgcct
      301 tatatcgcca gcacttcctt cacatttgaa acagaagtac catctgaaga tgactttacc
      361 gaccgcatta cctcatacct tgccaactgg ccctggcttg tttgtgaagt ggacgggcaa
      421 atagccggct atgcctatgc atcccgctac cgggagcggg tagcctatca atggtcggcg
      481 gaatgttcag tatatgttca ggatgatcat cacaggtcag gcattgcccg ggcattatat
      541 acggccttgt tttctatttt aaaattgcag ggctaccgga atgtttatgc ggtcatcaat
      601 ttgccgaatg aacgcagtgt tgcttttcat gaaagcatgg gcttccgttg gtttgctact
      661 tacgagcagg ttggttataa actggggcaa tggaaaaatg tgggctggtg gcggttaatc
      721 ataaatgaat ttgaacaaga gccggcaccg gtaataagct tttcactgct tgatcagtca
      781 tttgttccgc aattgttaaa tgacgttacc ggtttgttga ataaataaaa aaccgccccg
      841 gttcgcaggg cggctataat tccggaataa ttccgggtat agagaaaatc attttgatag
      901 cacagccttt tcttcttttg ccacaacttt tccgggatac tgctccagtg ctttctccaa
      961 acaatccata gcagcattga tggcatcaag atttaataca taagccagtc tcacttcatt
     1021 tttaccaagg ccggcagttc cgtaaaaacc agtggccggt gccagcatta ctgtttggtt
     1081 attgtaagaa aactcttcca gtaaccattg gcagaattta tcactgtcgt cgataggtaa
     1141 tttagcaatt gcataaaaag cgccgccggg attaggacag aatacgccct gcattgtatt
     1201 caaacgactt accagcaagt ctcttcttct ctggtattct ttcttgggag catcgaaata
     1261 actgtcggga agatctacgg ctgcttcacc catgatctgg gccagtcccg gagggcttag
     1321 ccttgcctgg gcaaatttca tggctgcatc ataaacggat ttgtttttag taacaaaagc
     1381 ccccagtctg gcaccacagg cactgtacct ttttgaaatg gtatccatta ataccacatg
     1441 ctgttccaat ccctgcaggt gcatagcact tacatagtca ccgtcatagc aaaactcacg
     1501 gtaggcttca tcactgaaga gatagagatt atgtttaatg cagatttctt tcaacgcttc
     1561 catttcctcc cggctgtaca aataacccgt agggttattg ggactgcaga tgatgatggc
     1621 ctttgtttta tcagtgatta ctttttcaaa gtcgtgaata ggagggaggg caaatccatt
     1681 gtcaatttta gaagtgattg ggactacatg cacaccggcg gcacaggcaa acccgttata
     1741 attggcatag aatggttccg ggatgataac ctcatcaccg gggttcatac aggtaaagaa
     1801 gccaaacagg atagcctcac ttccaccggt ggtaatcatt atctggtcat ggctgatatc
     1861 aatacctact tttttataat actgcaccaa tttgcggcga taactctcgt tcccggcact
     1921 gtggctgtac tccaatactt taatatcggc attacgcact gcatccagta tagccggtgg
     1981 tgtttcaata tcaggctgac caatattaag atgataaacc ttgattcctt tctttttggc
     2041 agcctcagca taaggcacca gtttacgtat aggcgaaggt ggcatcagct ggccacgttg
     2101 agaaatagtt agcatgcgac aaaggtaagt atctgttagt atcggtgcga tgtaatatca
     2161 tatgtacagt tgaccttggt tagccaagtt atcctgtttg ctggctactt tacgcagaaa
     2221 tttaccggac ttacaggata atagttgtat attatagatt tatctgaatg cagtctatcc
     2281 aaaaaatagt ttgtgaaacc aaagccatta atcctttttc cccatgccat tatgctggca
     2341 gttttggcct tgttctgtac ctgtacgggc agagataatg gattatctgg ttataaacat
     2401 gaatatgtaa ccgagactgc taagaccagg ataaatcccg ggttggcggg tttttccatt
     2461 tatagcactt ttgataatag tgatgaaata gcatctgcgg aagacaataa atctttaatg
     2521 agtttgctta aagagtatga aaaaattctc ccataataac tggtatgagc ctgtttcagt
     2581 caatagtaaa taagataaaa cccagaagat tacacannnn nnnnnnnnnn nnnnnnnnnn
     2641 nnagacttta ttgctgggtt tctttgctgt ttacataatc aagcaacaga agcaaattac
     2701 attaaagcaa aacagggaac aggcgaaaag cctggcccag gctttggctg taaatgccag
     2761 gttttatatt atttcacatg atgaagagat gctgctgaaa aacttgcagt caattaataa
     2821 gtttccagat cttgaatatg ctgctatatt agagaatgat ggaaaggtac tggcgcatac
     2881 caatcccgct tatttaggca tgaaactggc tgacagcatt ggtttaaagg gtattatgga
     2941 ttctgatggt agtataatct ttgagaacag caaagtggct gatgtaacct taccaattta
     3001 tgatgaaggc ggcagcagat taataggatg ggcaaaattg gccctgcaca gaagttcgat
     3061 ctcaaatgag tttagcatca ttgtcaggga ggtgacatta tacatgctgc ttggcctttt
     3121 aatcagtatt ataattgcct atttcattgc aaaaaatcta tcgaaggagc taaatattct
     3181 ttccggcctc actgaaaaaa ttgcacatgg ccaggtgaag gaaagggcag ctggctttaa
     3241 aaatatcgag gtagagcgac tgggaaaatc ttttaaccgg atgctggatg agttaaatac
     3301 gaatcagcag ttgatgaata ctattattga aaacctgccg gtaggtgtct ggcttttgaa
     3361 tgaaaaaggt gaaattattt cgagtaatac agaaggagaa cggatatggg ctggtgcaaa
     3421 gtatgtaggg agtgaacact ttggggaata caaaggatgg ttcctgaata ctggtaagaa
     3481 aattgaaccc gaagaatggg ctgctgcaag aattatgagg acaggaaagg cggtcctaaa
     3541 tgaagaactc gaaattgagt gtttcgataa aacaagaaaa atcattatca attcggcagt
     3601 tccacttttt gatgaagatc ataaaataag tggaggagta atcatcaatg ttgatattac
     3661 aggtatcagg gaaaaagaaa aggaattact gctggcacaa aagcaaattg atctgacaga
     3721 tgccaccatg aagattgcct ttgatcagtc gcctattgga atggcactgg tttctacaga
     3781 aggtaagctg ctcgaagcga ataaggagtt ttgccaaata ttgggctata ctgaagagga
     3841 aattgcaaag gtttcaattc aggatatttc acaccctgat gatatgcaaa tagatgtgga
     3901 gtatgctatg aaactactca ggggagaagt ggataaatat aaaattgaga agcgnnnnnn
     3961 nnnnnnnatc gaaaagataa aaccctggta tgggctaacc tgacggtaca cctggtacgt
     4021 cataagagcg gagttccgct ttatttcatt tcacaactgg aggatataac agaaagaaag
     4081 gccgcagaat taaagctaag agagagtgaa gaaaaattca gaacattaat tcagaaactc
     4141 cagataggca ttattaaaca caaggcagat acaagtattg agctattcaa tgacgccgct
     4201 ttacagcttt t
//
LOCUS       DAORLS010000264         4207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11492_length_4207_cov_1.951830, whole genome shotgun sequence.
ACCESSION   DAORLS010000264 DAORLS010000000
VERSION     DAORLS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11492_length_4207_cov_1.951830"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PK977_15895"
     CDS             <1..1159
                     /locus_tag="PK977_15895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF19657.1"
                     /translation="KMEQQEFRQYYRRLSEFFDTSLLRSGFNGGVLVAKNGNILYEKY
                     SGKVDIRKSDSITASTSFHIASTSKTFTGIATLRLVQEGKLSLSDTINKFFPGFPYTG
                     ITVKMLLNHRSGLPNYLYFMSNNKWGILPDGKWNKQMATNRDMLNMLIEKKPNVTGRP
                     DGKFTYSNTNYALLALIIEEVTGKSYPVYMKEKFFIPLQMNNTFVFTLKDTLTATPSF
                     TTTGTYWNNDFLDATYGDKNIYSTPQDLLKWDQALYTNQVLEQSWLDSAFKAYSFEKP
                     GIHNYGLGWRLQLLPNGKKVIYHFGKWHGNNAAFARLPDEKATVIILGNRFNRNIYNA
                     AHLCYDIFGDYMQRRQTETDDSPESTDKTPPAATKESKKATSTKPATKKKE"
     gene            1201..>1402
                     /locus_tag="PK977_15900"
     CDS             1201..>1402
                     /locus_tag="PK977_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRF19658.1"
                     /translation="MKILSAEQIRQWDQFTIEEEPIASIDLMERAAQKCTDWLCERFP
                     ETSSFSIFCGKGNNGGDGLAIAR"
     assembly_gap    1403..1538
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1539..2720
                     /locus_tag="PK977_15905"
     CDS             <1539..2720
                     /locus_tag="PK977_15905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRF19659.1"
                     /translation="QTEEHFHSFNKGTIIIDAIYGTGLNRPLEGVTANLVQHINNSGC
                     EIVSIDIPSGLFTDHSSKGNVMIHAGHILTFQCYKPAFFFAENDAAIDQVHLLDIGLH
                     QQFLEQISTNYEFVDDKLVHALYRPRKKFSHKGHFGHALIIAGSYGKIGAAVLATKAC
                     LRSGAGLVTSHIPSSGYTIMQDSVPEAMVMTDFNSSFVTKLSETPDRFKAIGIGPGIG
                     NAKESRDLLFDIFNSYEQPLVADADALNLMASEPELLRLLPAKSILTPHPKEFERLFG
                     TTENEFERLEKAKQKSAEFNCVIVLKGHHTAITLPNGTIFFNSTGNPGMATAGSGDVL
                     TGILTGLLAQGYSSFSAAILGVYLHGAAGDLAAKQNSMEAMVAGDIINNLGNAFLSLK
                     D"
     gene            complement(2721..3320)
                     /locus_tag="PK977_15910"
     CDS             complement(2721..3320)
                     /locus_tag="PK977_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19660.1"
                     /translation="MKKLTIFLVLIGASFIVYSQTTAITANGLRIIVFPNGTWINADS
                     ANFYGSQGNFNTDVKSEFISAYNFAFNELYSDVFFESERKQKAADWAGAGIRNGLTVY
                     AGSKSLTSWYDDLYHIAFNYMYSGVFFESERQKQANEWVRKILETRTIFDPYYYNSYF
                     KKYREAYTLAYNKIYSNEFFSADRKTKSRNWTNTFMTGK"
     gene            3587..>3895
                     /locus_tag="PK977_15915"
     CDS             3587..>3895
                     /locus_tag="PK977_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRF19661.1"
                     /translation="MDKLIYLVPVMGLIGLLYTFIKFNWVSKQDAGNEKMKEISNHIA
                     EGAMAFLKAEWKILGYFVVIVAILLAVMATTNPHSHWLIAGAFVVGAVFSATAGYIGM
                     K"
     assembly_gap    3896..3908
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3909..>4207
                     /locus_tag="PK977_15920"
     CDS             <3909..>4207
                     /locus_tag="PK977_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRF19662.1"
                     /translation="MKVATKANVRTAHAARTSLSKALNVSFTGGSVMGLGVAGLAVLG
                     LGGLFIVFKQIFAPTAAVNSEEMVRTIEVLTGFSLGAESIALFARVGGGIYTKAA"
BASE COUNT         1209 a          836 c          828 g         1185 t
ORIGIN      
        1 aaagatggag cagcaggagt tcaggcaata ttatcgcagg ctgagtgagt ttttcgatac
       61 cagtcttctg cggtcaggat ttaatggcgg tgtattggtc gcaaaaaatg gcaatatcct
      121 gtatgaaaaa tattcaggga aagttgatat ccggaaatca gattcgatta ctgccagcac
      181 atcttttcat attgcatcta ccagcaaaac tttcacaggc atagccactc tccggcttgt
      241 tcaggaaggc aaactttccc tgtcagatac aatcaataaa ttctttcccg gtttccccta
      301 taccggcatt acagtgaaaa tgttgctaaa ccaccgaagc ggccttccca attatctcta
      361 ctttatgagt aataataaat ggggtattct tccggatggt aaatggaaca aacaaatggc
      421 caccaaccgt gacatgctca acatgcttat tgaaaagaaa ccaaatgtta ccggcaggcc
      481 ggatggaaag ttcacctatt caaatactaa ctatgcatta ttggcactca tcatcgaaga
      541 agtaaccggt aaatcttatc ccgtatacat gaaagagaag ttctttattc ccctgcaaat
      601 gaacaatact tttgtattta cgctaaaaga tacacttaca gctacacctt catttaccac
      661 aacgggtact tactggaata atgatttcct ggatgccact tacggcgaca aaaatattta
      721 ttctaccccg caggatttat tgaaatggga tcaggcatta tacaccaacc aggtactgga
      781 gcaatcctgg cttgattctg ctttcaaagc ctatagtttt gagaaacccg gtattcataa
      841 ttatggactt ggctggcgtt tgcaattact ccccaatggt aaaaaagtga tttaccattt
      901 tggaaagtgg catggcaata atgcggcctt tgcccgactt cctgatgaaa aggccaccgt
      961 tatcattctt ggcaaccgat tcaacaggaa catttataat gcagcacatc tctgttacga
     1021 tattttcgga gattatatgc agcgccgcca gacagaaaca gatgatagtc ctgaatcaac
     1081 ggataaaact cctcctgctg caacaaaaga atcaaagaag gctacttcca caaaaccagc
     1141 tactaaaaag aaagaataaa tcattctttt tcccgatttt ctttgccaac tttatagcaa
     1201 atgaaaattc tttcagcaga acaaataagg caatgggacc aatttaccat tgaagaggag
     1261 ccaattgcat ccatcgatct gatggaaaga gcagctcaaa aatgtacaga ctggctatgt
     1321 gaacggttcc cggaaacttc ttcttttagc attttttgcg gaaagggcaa caatggtggc
     1381 gatggtctgg caatagcccg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca aactgaagag cactttcatt
     1561 ctttcaataa aggaacgatt atcattgatg ccatttatgg taccggtctc aaccggcccc
     1621 tggaaggggt aacagccaac ctggtacaac atatcaataa ttccgggtgt gaaattgttt
     1681 ccattgatat accaagtggt ttatttacag atcattcctc taagggaaat gtgatgattc
     1741 atgccgggca tatactcacc tttcaatgct ataaacctgc tttctttttt gcagaaaatg
     1801 atgcagcgat tgaccaagtc catttattgg acataggtct gcatcagcaa tttttagaac
     1861 agatctcaac aaattatgaa tttgtcgatg acaaactggt tcatgctctt tataggccaa
     1921 gaaagaagtt ctcgcataaa ggccattttg gccatgcact cattattgca ggcagctatg
     1981 gtaaaattgg tgcagcagtg ctggcaacaa aagcatgcct gaggagcggt gctggtttag
     2041 ttacttcaca tatcccctct tcagggtata caattatgca ggattccgtt cccgaagcca
     2101 tggtgatgac agacttcaat tcatcttttg ttacaaaact gtctgaaacg cctgacagat
     2161 ttaaagctat aggtattggg ccgggtattg gtaacgccaa agagagcaga gatttattat
     2221 ttgatatttt caattcttat gaacaaccgc tggttgcgga tgctgatgcg ttgaatttaa
     2281 tggcaagtga accagaactg ttacgtttac tacctgctaa gtccatacta acgccacatc
     2341 caaaagagtt tgaaagactt tttggtacga ctgaaaatga gtttgaaagg ttggaaaagg
     2401 ctaagcaaaa atcagccgaa ttcaattgcg ttattgtttt gaaaggacat catactgcaa
     2461 tcacactgcc caacggtact atattcttta attcaactgg caatcccgga atggccacag
     2521 ccggcagcgg tgatgtgctt acaggtatac tcactgggtt gctggcgcaa ggttacagtt
     2581 ctttttcagc agcaatttta ggcgtatatc ttcacggtgc cgcaggggat cttgctgcaa
     2641 aacaaaattc tatggaagcc atggtggccg gggatattat taacaatctt ggaaacgcct
     2701 ttctttcctt aaaagattaa tcactttccc gtcataaaag tattggtcca gtttcgggat
     2761 ttggtctttc tgtcagcaga aaaaaactca ttactataga tcttattata tgccagcgta
     2821 taggcttccc tgtatttctt aaagtatgag ttataataat aggggtcaaa aatggtacgg
     2881 gtttccagga tctttcttac ccattcattg gcctgctttt ggcgttcgct ttcaaaaaac
     2941 acaccgctat acatgtaatt gaaagcaata tgatacaaat catcatacca tgatgtcagc
     3001 gatttactgc ctgcatatac tgtaagccca tttcggatac ctgcaccggc ccaatctgca
     3061 gccttttgtt ttcgttcact ttcaaagaaa acatctgagt ataattcatt gaaagcaaaa
     3121 ttataagcac tgatgaattc actttttaca tcagtattaa aattgccctg agaaccataa
     3181 aagtttgcac tgtcagcatt tatccatgtt ccgttgggaa agacaataat acgcagcccg
     3241 ttggctgtaa tggctgttgt ttgtgaataa acgataaaag atgctccaat aagaacgagg
     3301 aaaatggtta gttttttcat gagtaatgtt tatagtctaa aataacggtt tatgataagt
     3361 cagcccattc ttgacggccg gatgtttggc ttggttgcca aactcgaatg ctaaagtata
     3421 ttcaaccaat gtcttagcca taaatttttg ccttaaacta tccgccggga acgttatcgt
     3481 gaaagccatt ttcccctatc tttgccgccc ttgattgcag caggcacccc cttaaatcga
     3541 tctggtgatt aatgcaatca ttaaatcttg aataagaata cactgtatgg acaaactgat
     3601 ttatctcgtc cctgtaatgg gcctgattgg gttactctac acatttatca aatttaactg
     3661 ggtatctaag caagatgccg gtaacgagaa aatgaaggaa atcagcaatc acattgctga
     3721 aggtgcaatg gctttcctta aagcagagtg gaaaatactg ggctactttg tagtaattgt
     3781 tgctatcctg ctggcagtga tggccacaac caatccgcat tcacattggc ttattgccgg
     3841 agctttcgtt gttggagccg tttttagtgc tactgccggt tacatcggaa tgaaannnnn
     3901 nnnnnnnnat gaaagtagct acaaaagcga atgtaagaac tgcacatgcc gcccgtacaa
     3961 gccttagtaa agcattgaat gtttctttca ccggcggttc agtaatgggt cttggtgtag
     4021 ccggtctcgc tgtactggga ttaggtggtc tttttatcgt tttcaaacaa atatttgcac
     4081 ccactgctgc tgttaactct gaagaaatgg taagaaccat tgaggtgtta accggatttt
     4141 cattaggtgc tgaatcaata gctctgtttg caagggttgg tggtggtatt tataccaaag
     4201 cagccga
//
LOCUS       DAORLS010000265         4198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11523_length_4198_cov_2.278059, whole genome shotgun sequence.
ACCESSION   DAORLS010000265 DAORLS010000000
VERSION     DAORLS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_4198_cov_2.278059"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..>410
                     /locus_tag="PK977_15925"
     CDS             150..>410
                     /locus_tag="PK977_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19663.1"
                     /translation="MRSRNWDDAVRLLKKAVRKSPYSSAYADKLKEAEAALEKERQQN
                     RDLQEKIRQQEEAKKKAEEDKRRAEEARLEKIRQEQAVIKAKL"
     assembly_gap    411..464
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <465..1208
                     /locus_tag="PK977_15930"
     CDS             <465..1208
                     /locus_tag="PK977_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19664.1"
                     /translation="EAQLQLKNYSKALLNNNSELEKWGQQVDQSYNSVLDNSKEYLAG
                     LFIKYNLMGALKKQLHADVYKKMGNLWKSSNPEIQKWLAKNLAAADLKADKVEEVVDF
                     VSKGGDLAELLTADKEEAGYNLKVLLFLNGVMESSGLSGYEKLMENALGAMPGDYFEQ
                     AKMIGETYADLAAICYSWFSIRKITASNEEMAEKVKILSAGIEQRMEEIECLKKCMKN
                     YTSRCLENCTGKTRWSTPPPPLLFNYRKW"
     gene            1212..3509
                     /locus_tag="PK977_15935"
     CDS             1212..3509
                     /locus_tag="PK977_15935"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF19665.1"
                     /translation="MKTVLSGLLSFVILIHATAFRLSAQLSGGMQDFIESFLQINKSP
                     VKNSNDLSGYYLQPQMHNGKWGFIDTRTGDTAIHFRYDSINYFSEEMAAVMQQGKWGF
                     INKYGKLLIPFRYDSIGYFQDNLCFVSKGKNVGYINKSGKEIIPVQFSEVYYVDDTLV
                     RVMKDKKYGLFNLAGKQLSPPRFDDMTNFYEDRAWVEINGKWGFINKEGVLLISPKYE
                     MFNRFTEGLAWVRSESGLYGYVNKNGDEVVYPMYEEAQSFSEGLAAVKTYMEGGKLSY
                     IYINKNGESVISNIGAGIDEAYPFKNGYAKVMSGIRYGLIDKTGRTIIPLIYKDMGEL
                     SDSMISVKEDDDKWGYEKVYYDNLLKTTMSDRVIPCKFDEAYNFENGLAMVKSGDAYF
                     INKKGEYVKPVKEVEDEEITNNTGEQTESISIDYINLRGERIIRGKPGNMSGLFLNSA
                     ALVQMDGKWGYMNKQGAIIIPCRYDSAGSFNEGLATVKQNNKWGFIYQDGDTLTSCRF
                     DYAYNFHNGIAIIKMNGKYGYIDKTGLILIPCQFDMAYTFKDSVAIIRNGDQFGFINK
                     AGRLVIPAEYDDAFDFHEGLAAVQKDGLWGYINKNGVVVIPFRYENAGRFAEGLAPVM
                     KNGKYGYIDIAGNTIVPYVYKFAYPFVNGKAIAGEENEEIVVLNTKGNEVFAAQLRSL
                     FYEDLAIVVNDKGKLGYMNQNGQTVIACQYDDAMIFREGLAAVAINKKWGFINKQGTV
                     VIPLQFDAALVFNEGIGIIANIKND"
     gene            3511..3897
                     /locus_tag="PK977_15940"
     CDS             3511..3897
                     /locus_tag="PK977_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19666.1"
                     /translation="MKKISDSITFSTVRNMAFIGAAILFAFLLLGFDRQQNRSEKLLG
                     ITFDDEKKEVTIKVTSTGCTEKNYFQFKVRGTVIEIIRTKRDECKRMPFTTSFTFSMA
                     ETGLVPNKVYTIKNKFSASTDLANLP"
     gene            complement(3898..>4198)
                     /locus_tag="PK977_15945"
     CDS             complement(3898..>4198)
                     /locus_tag="PK977_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF19667.1"
                     /translation="DEERPAWGFPDVLAPSFSEPYKNGMRKVTGGDAYICFVRYPRDG
                     GLPKIESVNTFGASMDPSSPHFKDQWGMFQAQQTKPMTLDKTEVLLKAKAVYHPG"
BASE COUNT         1377 a          741 c          942 g         1084 t
ORIGIN      
        1 tacataagcc cagatttcca taagaaatta tcccgtatcg tctttctgct cggagaggga
       61 gaaattacac ttaccgatta cctttttcaa aaacaccgga acaaaaatct gccgaagcag
      121 cagatcatta tgccgatgca ctgaaagcca tgcgaagccg caattgggat gatgccgtca
      181 ggttgttgaa aaaagcagtt cgaaaatcac cctacagttc tgcttatgcc gataaattaa
      241 aagaagctga agcagcgttg gaaaaagaaa ggcaacaaaa ccgggactta caggaaaaaa
      301 taaggcaaca ggaagaagca aagaaaaaag ctgaggaaga caaaagacga gcagaagaag
      361 caaggctgga aaaaatcagg caggaacagg cggttattaa agcaaaactt nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaagcg cagttgcagt
      481 taaaaaatta ctcaaaagcc ttactcaata ataattcaga actggaaaaa tgggggcagc
      541 aggtagacca atcctacaac agtgtgctgg ataacagcaa ggaatacctg gccggcttat
      601 tcatcaaata caacctgatg ggtgcattga aaaaacagct tcatgcagat gtgtacaaaa
      661 agatgggcaa cctatggaag tcatcaaacc cggaaataca aaaatggctg gcaaaaaatc
      721 tggccgcagc agatcttaag gcagataaag tggaagaagt ggttgacttt gtatcaaagg
      781 gtggagatct tgctgaattg ctgacggcag ataaagaaga ggccggttat aacctgaaag
      841 tgttgttgtt ccttaatggt gtaatggaaa gctctggctt atccggttat gaaaaactaa
      901 tggagaatgc cctgggtgct atgcccggcg attactttga acaggcaaaa atgattggcg
      961 aaacctatgc agatcttgcg gccatttgtt atagctggtt tagtataaga aagattactg
     1021 cttctaacga agaaatggcg gaaaaagtaa aaattctttc cgccggaata gaacaacgga
     1081 tggaagaaat agagtgcctg aaaaaatgta tgaaaaatta taccagccgc tgccttgaaa
     1141 actgtacagg taaaaccagg tggagtacac ctccgcctcc gctacttttt aattaccgaa
     1201 aatggtgaga tatgaaaact gttttatccg ggcttctatc attcgtcata ctcattcatg
     1261 ccactgcctt tcgcttgtca gcacagttat ccggtggtat gcaggatttt atcgaatcct
     1321 tcctgcagat caataaatca ccggtcaaaa attccaatga tctctccggt tactacctgc
     1381 agccgcaaat gcataacgga aagtggggat ttatcgatac caggaccggt gatacagcta
     1441 ttcacttcag gtatgacagc atcaactact tcagtgaaga gatggcagct gtaatgcagc
     1501 agggcaaatg gggttttatt aataaatatg gaaaattgct tattccgttt cgctatgatt
     1561 caataggtta ttttcaggat aatctttgtt tcgtttcaaa agggaaaaat gtgggttata
     1621 tcaacaaaag cggaaaggag attattcctg ttcaattcag cgaggtgtac tatgttgatg
     1681 atacccttgt aagagtaatg aaagacaaaa aatatggatt gttcaatctt gcaggaaaac
     1741 agttaagccc tccccggttt gatgatatga ccaattttta tgaagaccgt gcctgggtgg
     1801 aaataaacgg aaagtggggt ttcattaaca aagagggtgt tttgctgatc agtccgaaat
     1861 atgaaatgtt caaccggttt actgaaggcc tggcatgggt aagaagcgaa tccggacttt
     1921 atggttatgt aaataaaaac ggcgatgaag tggtgtatcc catgtacgaa gaagctcaat
     1981 cattcagtga aggactggcc gctgtaaaga cctatatgga aggtggtaaa ctttcgtata
     2041 tctatattaa taaaaatggt gaatcagtaa tttccaatat tggtgcaggt attgatgaag
     2101 cctatccttt taagaacggg tatgcaaaag tcatgtctgg tattcgttat ggcttaattg
     2161 ataaaacagg cagaacaata ataccgctaa tttataagga tatgggagag cttagtgaca
     2221 gtatgatttc cgtaaaagaa gatgatgaca aatggggata tgaaaaagta tattacgaca
     2281 acctgctaaa aacaacaatg agtgacaggg ttataccctg taagtttgac gaagcctaca
     2341 attttgaaaa cggactggca atggtaaaaa gcggcgatgc ttattttatc aataaaaaag
     2401 gggagtatgt aaaaccagta aaagaagttg aagatgaaga aataactaat aataccgggg
     2461 aacaaaccga aagcatttcc attgactata tcaacttacg gggcgaaaga atcatacggg
     2521 gcaagcccgg aaatatgtcg ggtcttttct taaatagtgc cgcattggta caaatggatg
     2581 gaaagtgggg ttatatgaac aagcagggag caataataat tccctgccgg tatgacagtg
     2641 ccggttcctt taatgaagga cttgcaacag taaagcagaa caacaaatgg ggctttattt
     2701 accaggatgg cgatacactt acttcctgtc gttttgatta tgcttataac tttcacaatg
     2761 gtatagctat cattaaaatg aacgggaagt acggctatat tgacaaaacc ggtcttatcc
     2821 tgattccttg ccagtttgat atggcctaca catttaaaga cagtgtggcc attattcgaa
     2881 atggcgacca gtttggtttc atcaataaag caggcagatt ggtaataccc gcagaatatg
     2941 atgatgcatt tgattttcat gaaggcctgg cagccgtaca aaaggacggc ctgtggggat
     3001 atatcaataa aaacggggtc gtcgtcattc catttcggta cgaaaatgcg gggcgattcg
     3061 ctgaaggcct tgcgccggtg atgaaaaatg gcaagtatgg atatatagat attgcaggta
     3121 atactattgt tccctatgtg tacaaattcg cctacccgtt tgtaaatggc aaagccattg
     3181 caggagagga aaatgaggaa atagtagtac tgaataccaa aggaaatgag gtctttgctg
     3241 cacagcttcg aagtttattt tatgaagatc ttgcaattgt tgtaaatgat aaaggcaaat
     3301 tgggttacat gaatcagaac ggacaaaccg ttattgcctg ccagtatgat gatgccatga
     3361 tattccggga aggattggct gccgttgcga ttaataaaaa atggggtttt atcaataaac
     3421 aaggaacagt cgtcattcct ttacagtttg atgctgctct tgtctttaac gaaggaatag
     3481 gaattattgc caatattaaa aacgactaac atgaaaaaaa ttagtgacag cattacattc
     3541 agcactgtcc gtaatatggc ttttattgga gccgctattt tatttgcatt cttgcttctt
     3601 ggttttgatc gtcagcaaaa ccggtccgaa aaattactgg gtattacttt tgatgatgag
     3661 aaaaaagaag taaccatcaa agtaaccagt accgggtgta ctgaaaaaaa ttattttcaa
     3721 ttcaaagtga gaggcactgt gattgagata ataagaacta aaagagatga atgtaaacga
     3781 atgcctttta caactagctt cactttcagc atggcagaaa caggcctggt gcccaacaag
     3841 gtttacacta taaaaaataa atttagtgca agcaccgacc tggctaattt accatagtta
     3901 tcccgggtga taaacagctt tggcctttaa aagcacttcc gttttatcca aggtcatagg
     3961 cttggtttgc tgcgcctgga acattcccca ctgatcttta aagtgaggcg atgagggatc
     4021 catcgatgca ccaaatgtat tcaccgattc tatttttggc aaccctccgt ctctgggata
     4081 acggacaaaa cagatataag catcaccacc tgttactttt cgcattccgt ttttataagg
     4141 ctcagagaag gaaggcgcaa gcacatccgg aaaaccccat gccggtcttt cttcgtcg
//
LOCUS       DAORLS010000266         4188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11567_length_4188_cov_1.818292, whole genome shotgun sequence.
ACCESSION   DAORLS010000266 DAORLS010000000
VERSION     DAORLS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11567_length_4188_cov_1.818292"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>783)
                     /locus_tag="PK977_15950"
     CDS             complement(<1..>783)
                     /locus_tag="PK977_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19668.1"
                     /translation="ASVTTGSASAITQTTATAAGSITATGCSSVTAYGIEYSTTNNFP
                     LGTGTQVASSNLSGGNFSAGLTGLAASTTYYYRAYATNAGGTAYGNQQSFTTAAPTLT
                     ASALVPFGNICINTTSAANSFTITGVGLTNANVNVGPLAGYSFSTTSGGTYTASLSLT
                     QPGGAFSQAIYVKLSPVLVQAYTGNIPVSGGGAASTSVAADGSGVNTLATVVTGASTN
                     ITNHSATLAGSIAATGCSNVTEYGIEFSGINGFGNGTGTKVVS"
     assembly_gap    784..811
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<812..1868)
                     /locus_tag="PK977_15955"
     CDS             complement(<812..1868)
                     /locus_tag="PK977_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19669.1"
                     /translation="MRKTNGCTDTDNNNGDFDVVIAPVPRNSASPVVICGSVSPSLTI
                     SSPLASFGNTCINTTAGPNSFTISGSNLTTANVAVAALTGFEYATASGGPYSTTLSLT
                     QPGGSFSQQVFVRFTPTAVQSYNGNIAVSGGGVTTPVNAATVGSGVNTAPSVTTGGAS
                     SITLTSATAAGSIPSTGCTAITAYGIEYSTTGGFTPGTGTQVASTNIAGGNFTSDLTG
                     LSSGTTYYYRAYATNGGGTTYGAEQSFATLTPNPTITTSALSAFGNVCINTTAGPNSF
                     TITGSNLTTANVTVGPLTGYTFSTTAGGTYTASLSLTQPGGSYSQQIFVRFTPTAVQS
                     YNGNIPVDGGGIAAPVNV"
     assembly_gap    1889..2018
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2019..2440)
                     /locus_tag="PK977_15960"
     CDS             complement(<2019..2440)
                     /locus_tag="PK977_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF19670.1"
                     /translation="MKKIFTACFILFSLITFSQSTTVVISQVYPGGGSSSPTVTYKND
                     YVELHNISSVVQDISGFSLQYGSATGNFGSSGTQIFAIPAGTTIPAGGYLLIQLGTAG
                     TGGANLPVTPDLSSTNLSMGAASGKVALVNNSTPLGCGA"
     gene            complement(2488..3951)
                     /locus_tag="PK977_15965"
     CDS             complement(2488..3951)
                     /locus_tag="PK977_15965"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRF19671.1"
                     /translation="MAAVVLFASLFIVSCEKKFTDPPPIGEPDVVANISIKDVKARYT
                     SGAPVQITDDAIIEGVVSCDDRSGNYYQQIAIQDSTGGVLLRLGSFNLYNYYPVGRKV
                     YVKLKGLYLGQYNGTLQFGGGIDSAYINQGGVTLLAANLMDKHIIKGALNQPLVPKVV
                     TVSQLTTNLQDKYVSTLIKLVGYEFASSDRNKNYADDNASGNRTLQDCSNVSANRITL
                     RTSNYSNFATIPVAQGNGEIIAVYSFFGSTKQLTIRDTTDVRFYNPRCPTASGDGQIS
                     LTTSPITFNFDNLGTAGLPTGVYVKESASSSYIGNDGFVYQSNFNNGTAWNQTSAGFK
                     NFASATGLNSTASTSTQASAANRALGYRQTGTFGDPGAAFAFLLSNTTGKSNLQLEFK
                     LQSLDAPVTLGRTTTWRVDYATGLNPAFFSTVATSPATITTTNGTFSNTTVTVNFGSL
                     LNNITQPVWIRIVTLTNTSGGGNRPSTGIDDVRFSWN"
BASE COUNT         1134 a          899 c          899 g         1098 t
ORIGIN      
        1 agacacaact ttagttcctg tgccgtttcc aaagccatta ataccactga attcaattcc
       61 gtactctgta acgttggaac aacctgtggc agcaatagat ccggccaaag tagcagaatg
      121 attggtaatg ttggtggaag cacctgttac cacggtagcc aatgtattca caccactgcc
      181 atcagcagca actgacgtgg aagctgcacc accaccgctt accggtatat tacctgtgta
      241 tgcctgaacc agtaccggcg ataattttac ataaatagcc tggctaaaag cgccgccggg
      301 ttgggttaag cttagtgaag cagtgtatgt accaccgctg gttgtagaga atgaatagcc
      361 ggccaacgga ccaacgttta cattggcatt ggtaagacca acaccagtta tggtaaatga
      421 atttgccgct gatgtagtat tgatacaaat attaccaaaa ggtacgagtg ctgaagccgt
      481 taatgtgggt gccgcagtag taaagctctg ctgattgccg taagcagttc cgccagcatt
      541 ggtggcataa gccctgtaat aataagtggt gctggccgca agaccggtca gccctgcact
      601 gaagttacct ccggacaaat ttgaagaggc tacttgtgta ccggttccca gcgggaagtt
      661 attggtagta ctatattcaa ttccatacgc tgttacgctg gaacaaccgg ttgctgttat
      721 agaacctgct gcagtagcag tggtctgcgt aatggcactg gcagaacctg ttgtaacaga
      781 agcnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaacattca ccggtgcagc tataccacca
      841 ccatctaccg gaatatttcc attataagat tgaacagcag ttggcgtaaa tcttacaaaa
      901 atctgctgag aataagaacc gcccggttgt gtcaaactca atgaagcagt ataagtgcca
      961 ccggcagttg tagagaaggt gtacccggtg agcggaccaa ctgtaacgtt tgccgtagtc
     1021 aggttgctgc cggtaatggt aaatgaatta ggtccggctg ttgtattgat acacacatta
     1081 ccaaacgcac taagcgctga tgttgtaatg gttgggttcg gtgtcaaggt agcaaatgat
     1141 tgctccgcac cataagtagt gcccccgcca ttagtagcat aagccctgta gtaataggtg
     1201 gtgccggaag aaagacccgt gagatcagaa gtgaagttac ctcccgcaat attggtagag
     1261 gcaacttgtg tacctgttcc cggagtgaaa ccgcccgttg tactgtactc aataccatag
     1321 gcagtgattg ctgtacatcc tgtagatgga atgcttccag cagcagtggc agaagtaaga
     1381 gtaatagaac tggctccgcc tgttgttacg gacggagcag tattgacgcc tgatccaaca
     1441 gtagcagcgt taaccggtgt agttacacca ccaccgctta cagcaatgtt tccgttgtaa
     1501 gattgaactg ctgtaggtgt aaaccttaca aatacctgct gactaaatga gccacccggc
     1561 tgagttaaag ataaagttgt agaataggga ccgcctgatg cagtggcata ttcaaatccc
     1621 gtcaatgcag ccacagctac atttgctgta gtaaggtttg aacctgaaat ggtaaatgaa
     1681 ttaggaccag cagtagtatt gatacaagta ttcccaaatg atgcaagtgg cgaggatata
     1741 gtcaaagaag gacttacact tccacaaatt acaactggag aagcactgtt cctcggaact
     1801 ggcgcaatca caacatcaaa gtcaccatta ttattatcag tgtctgtgca gccatttgtt
     1861 tttctcacac acccgtttgt attgcttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tccacaaccc aggggagttg
     2041 agttattcac caatgctact ttaccagatg ctgcccccat acttaagttt gttgatgata
     2101 aatctggcgt aactggtaaa ttagccccgc ctgttcctgc tgttcctaat tgtataagca
     2161 aatatccgcc agctggaata gtagtcccag caggaatagc aaatatctga gtgcctgaac
     2221 ttccaaagtt gccagtagcc gagccgtact gaagcgaaaa tccacttata tcctgaacaa
     2281 ctgaactaat attatgcaac tcaacataat cgttcttata tgtgactgta gggctgctac
     2341 ttcctcctcc cggatatacc tgactaataa ctactgttgt tgactgagaa aaagtaataa
     2401 gcgaaaaaag aataaagcaa gccgtaaata tttttttcat ttcttttatt ttaatcattg
     2461 aaattgcgtc ttacgctcct ttaaaattta attccagcta aaccttacat catcaattcc
     2521 tgtcgacgga cgattaccac cacctgatgt attggttagg gtaactatcc ttatccatac
     2581 cggttgcgta atgttattca gcaggcttcc gaaattcact gttacagtag tatttgaaaa
     2641 agtgccattg gtggtagtga tcgttgccgg gctggtggca acggtagaaa agaaagcagg
     2701 attcaaacct gtagcataat ctacccgcca ggtagtagta cggccaagcg taacaggcgc
     2761 atccagcgac tgcagcttaa actccagctg gaggtttgac ttacctgttg tgttggagag
     2821 caggaaagca aaagcagcac ccggatcacc aaacgttcct gtttgcctgt accccaatgc
     2881 cctgttcgct gcagaagcct gggttgatgt agaagcagta gaattcagcc ctgttgccga
     2941 tgcaaaattc ttaaaacctg cagaagtctg attccaggca gtgccattat taaagtttga
     3001 ctggtaaaca aatccatcat ttcctatata cgaactagaa gcactctctt ttacgtaaac
     3061 tcctgttggc aatccagctg ttcccaggtt atcgaagttg aatgttatag gtgatgtggt
     3121 gagactgatc tggccatctc cgctagcggt agggcaacgg ggattataaa accgaacatc
     3181 tgttgtatct cttatagtca actgcttggt tgaaccaaag aatgaataaa cagcaatgat
     3241 ctcaccattt ccctgtgcca ccggtatggt tgcaaaattg ctatagttgc tggtacggag
     3301 agtaatcctg ttggctgata catttgaaca gtcttgtaat gttctgttac cagaagcatt
     3361 atcatcagca taattcttat tcctgtcaga agaggcaaat tcatagccta ctaatttgat
     3421 cagtgtgcta acatatttat cctggaggtt agtggtcaat tgtgatacag taaccacttt
     3481 gggtaccaaa ggctggttta atgctccctt aataatatgc ttatccatca gattagcagc
     3541 cagcagtgtt acacctccct ggttgatata agcagaatcg ataccaccgc caaactgtaa
     3601 tgtaccattg tactgtccaa gatataagcc tttcagcttt acatatactt tacggcctac
     3661 ggggtaatag ttgtacaggt taaaacttcc caggcgaagt aacacacccc ctgttgaatc
     3721 ctggattgca atctgctggt aatagttacc gcttctgtca tcacaggaaa caacaccttc
     3781 gatgatcgca tcatcggtga tctgtacggg tgccccggat gtataccggg cttttacatc
     3841 cttgatgctg atattcgcca ccacatcagg ctcaccgata ggaggcggat cggtaaattt
     3901 cttttcgcat gatacaataa ataaactggc aaacagtaca actgctgcca tgcttatccg
     3961 attgtgctta ctgttcattt gcataaaatt atttttcaga gttcagtttt aatttattga
     4021 atatccgcag cggtacggat tccgatctgg gtggtcgtat tgtaaatcga aacatacccg
     4081 gttaccgatg taccacttgt atttaccgta atgccggatg catttcttac aaacatggac
     4141 agggtaccgg ttgcatctgt aacattgtag gtaacaccgg ttgggtta
//
LOCUS       DAORLS010000267         4177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11600_length_4177_cov_1.640466, whole genome shotgun sequence.
ACCESSION   DAORLS010000267 DAORLS010000000
VERSION     DAORLS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11600_length_4177_cov_1.640466"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PK977_15970"
     CDS             complement(<1..647)
                     /locus_tag="PK977_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF19672.1"
                     /translation="MKPPHKRIWLLPVLVTLFTFQVFPANAQNRREKVQSDYKKNPLD
                     ANIIASDTFMHRLLAENSQYFSEILEKKEELGLQVIYTRIDRTKKNKPGFTDHYFGVN
                     ADKYFYPASTVKLPVAILTLQRLNELNIAGLDKNSTMYTASAGERLTAVANDPSAQDG
                     RPTVAHYIKKILLVSDNDAYNRLYEFLGQEYINTSLQKMGYVHTKIIHRLSISLS"
     assembly_gap    822..854
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <855..>1093
                     /locus_tag="PK977_15975"
     CDS             <855..>1093
                     /locus_tag="PK977_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19673.1"
                     /translation="MQLKGLVRFFTVLLIIYSLYQLSFTWFVKSHEKKYEAKAKAYVK
                     ANYPSAMEKFPGNKDSQAVYQEFLEKVYQERLRRL"
     assembly_gap    1094..1123
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1124..>1721
                     /locus_tag="PK977_15980"
     CDS             <1124..>1721
                     /locus_tag="PK977_15980"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRF19674.1"
                     /translation="VTVTYGINGSVSYQKAKEEELNLGLDLQGGMNVTLEVERSGLLR
                     SLANNSKDPNFNTALVNADKRKANSGADFITLFLEEYRKLEKDRPLASLFAGASNGKI
                     DVKSSDAAVESYIQSEAKDAFDRTFRVLNTRIDQFGVAQPNISPNRDKGIINVELPGI
                     KDKERVRKYLQSTANLQFWEVYTLQDPNYSAGWQKAINA"
     assembly_gap    1722..1828
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1829..>2074
                     /locus_tag="PK977_15985"
     CDS             <1829..>2074
                     /locus_tag="PK977_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19675.1"
                     /translation="TTLAGLADDKDTANNKTGDLTTTSSNTIDKYISFFSPGQNEQGQ
                     VVYPASIGNVQIKDTAQVRIYLNELKANFPFDVQWAY"
     assembly_gap    2075..2230
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2231..>2921
                     /gene="secD"
                     /locus_tag="PK977_15990"
     CDS             <2231..>2921
                     /gene="secD"
                     /locus_tag="PK977_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRF19676.1"
                     /translation="EGRMDMKNFGATIWKKMTEENVGKPIAIVLDNIVYSAPNVINAI
                     PNGTSTISGGFDQQEAQDLANILKAGKLPAPAKIVQEQQVGPTLGAEAIRGGSMAFII
                     SFVVIFLLMLVYYNTSGWIANIALVLNLLFTVGILAGLGATLTAPGIAGLVLTIGMAV
                     DTNVVIYERIKEELTRGKGYIPAINDGYKRSLPPVLDGHVTTLLTAIILFYFGLGPVK
                     GFATTQIIGLLL"
     assembly_gap    2922..2958
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2959..>3364
                     /locus_tag="PK977_15995"
     CDS             <2959..>3364
                     /locus_tag="PK977_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRF19677.1"
                     /translation="ILVSRWISDIFTNKKKHLEYFTGVSRKIFKHAKYKFIEYRKVAY
                     IISFVVLSLGIASFFNGFSYGVEFNGGRSYTIQFDKKVEVEKVRDDLNKSFDGENTII
                     KTIGDNSKLDITTSYLIKDSRPISDSLVVVKLY"
     assembly_gap    3365..3401
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3402..>3784
                     /locus_tag="PK977_16000"
     CDS             <3402..>3784
                     /locus_tag="PK977_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRF19678.1"
                     /translation="EQFNTDKYIGQSKKVLPTISDDLKAGALKATLFALVVIFLYIFI
                     RFRDWRYSLGTIVALLHDVLVTLIVFSFARSIVPFPLEIDQHFIAALLTVIGFSMNDT
                     VIVFDRIREDSRLMPNAPRGEIINR"
     assembly_gap    3785..3804
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3805..4047
                     /locus_tag="PK977_16005"
     CDS             <3805..4047
                     /locus_tag="PK977_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19679.1"
                     /translation="ETLSRTIMTSLTVFLTLLILFLVGGEVTRGFAFAMLVGVITGIY
                     SSVFVAAPILVDLGGKRSLGKKTIEKTVKTEEAAKP"
BASE COUNT         1113 a          753 c          794 g         1097 t
ORIGIN      
        1 tcagataaag agatactaag cctatgaata atttttgtat gaacatagcc catcttctgt
       61 aaactggtgt tgatgtattc ctggccaaga aactcataca accggttata ggcatcgtta
      121 tcactgacca gcagtatttt tttgatataa tgtgcaacgg taggtctgcc atcttgtgcc
      181 gaggggtcat tggcaactgc agtaagcctt tcaccggctg aggccgtgta catcgttgaa
      241 tttttgtcca ggccggcaat atttaactca ttaagccttt gtagtgttaa aatggctacc
      301 ggcaatttga cagtcgaagc tggataaaaa tacttgtctg cattgacccc aaaataatga
      361 tcggtaaatc cgggtttgtt cttttttgtt ctgtcaatac gggtgtaaat gacttgcagt
      421 cctaattcct ccttcttctc aaggatctct gaaaaatatt gagaattttc tgctaaaagc
      481 ctgtgcatga aagtgtcaga ggcgataata ttagcgtcca atggattttt tttatagtca
      541 gactgcactt tttctctacg attttgagca tttgccggaa atacctgaaa agtaaaaaga
      601 gtaaccaaaa caggtaaaag ccatatcctt ttatgcggcg gtttcatcac ccctcaaata
      661 taccaattgt gcccagttaa ggttttaaaa atcaaggggc aagaacctgt ttttagttgc
      721 cgggaattaa tccgaaacag ctatctttgc cgtccttaaa aaatagtgta aagcctttgt
      781 cctgctgagt tacaaccgga acaaagctta aaaatgtaaa tnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnntatgca actgaaaggt ttggttcgtt tttttaccgt actgcttatc
      901 atctactcat tgtaccagct ttcttttacc tggtttgtaa aaagccatga aaaaaagtat
      961 gaagcaaagg caaaagctta tgtaaaagca aattatcctt ccgccatgga gaagtttcct
     1021 ggcaataagg attcccaggc tgtataccag gaattcctgg aaaaagttta ccaggaaaga
     1081 ctgcgcagat tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgtaac agtaacatac
     1141 ggcatcaatg gatctgttag ttatcaaaag gcaaaagaag aggagttgaa tcttggtctt
     1201 gacctgcaag ggggtatgaa cgtaacgctg gaagtggaaa gatctggctt actccgctca
     1261 ttggccaata attcaaaaga cccgaatttc aatacagccc ttgttaatgc tgataaacgc
     1321 aaagccaaca gcggcgctga ttttattacg cttttcctgg aagaatacag gaaactggaa
     1381 aaggacagac cattggcgtc gttatttgca ggtgccagta acggtaagat tgatgtaaag
     1441 tcttctgatg ctgcagtaga aagttacatt caaagtgaag caaaagatgc attcgacaga
     1501 actttccgtg ttttaaatac ccgtattgac caatttggtg tagcacaacc caacatcagc
     1561 cccaacaggg ataagggaat tatcaatgta gagttacccg gtattaagga taaagaaagg
     1621 gtaagaaagt atttgcaaag cacagccaac ctgcagttct gggaagttta tacattgcaa
     1681 gaccctaact acagcgctgg ctggcaaaaa gccattaatg cnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat accacccttg ctggtcttgc agatgataag
     1861 gacaccgcca ataataaaac aggtgactta accactacca gttccaatac aattgataag
     1921 tatataagct ttttctcacc tggtcagaac gagcaaggcc aggttgttta tcctgcatct
     1981 atcggtaatg tacagataaa agatactgcc caggtcagaa tatacctgaa tgaactgaaa
     2041 gcaaactttc catttgatgt acagtgggca tacgnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn ttgagggaag aatggacatg aagaattttg gagcaactat ctggaagaag
     2281 atgacagaag aaaacgttgg caaacccatt gccattgttc tcgacaatat tgtttattcc
     2341 gctccaaatg ttattaacgc tattcctaac ggaacttcta ccatttccgg tggttttgac
     2401 cagcaggaag cacaggatct tgccaatatt ttaaaagccg gtaaactgcc tgctcctgcc
     2461 aagatcgttc aggaacagca ggtaggccct acattgggtg ctgaagcaat tcgaggcggg
     2521 tctatggcat ttatcatttc attcgttgtc atcttcctgc tgatgttggt ttattacaat
     2581 accagcggat ggatagctaa tattgccctg gttttaaacc tgcttttcac tgtaggtatt
     2641 ctcgcaggcc ttggtgctac acttactgcg cctggtattg ccggtctggt attaaccatc
     2701 ggtatggcag tagataccaa cgtggtgatc tacgaacgaa tcaaggagga attaacaaga
     2761 ggtaaaggat acatacctgc gatcaatgac gggtataagc ggtctttgcc acctgttctt
     2821 gatggtcacg tcaccaccct ccttacagcg attatcctgt tttactttgg tcttggcccg
     2881 gtaaaaggat ttgccacaac acagataatt ggtttattac tnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnat cctggtcagc cgctggattt cagacatctt taccaataag
     3001 aaaaaacatc ttgaatattt tacaggagtt tcccgcaaaa ttttcaagca tgccaagtat
     3061 aagttcattg aatacagaaa ggtagcctat atcatttctt ttgtagtctt atcactgggt
     3121 attgcgtcct tctttaatgg atttagttat ggggttgagt tcaatggcgg aagaagctat
     3181 accatccagt ttgataagaa agtagaagtg gaaaaagtga gggatgacct gaataaatct
     3241 tttgacggcg agaacaccat tattaaaaca atcggtgata actctaaact ggatatcacc
     3301 acttcttacc tgataaaaga ttcaaggccc atttctgact ctttggtggt ggtaaaacta
     3361 tatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgaacagt ttaatacaga
     3421 taaatacata ggccagagca agaaagtact tccaactatt tccgacgacc ttaaagccgg
     3481 ggccctgaag gctacgcttt tcgcccttgt ggttatcttc ctttatatct tcattcgctt
     3541 ccgtgactgg aggtattcac tgggtaccat cgtagccttg ctgcatgacg tattggtaac
     3601 gttgattgta ttctcattcg ccagaagcat cgtccctttc ccgctggaaa tagaccagca
     3661 tttcatagcc gctctgttaa cggtgattgg tttctcgatg aatgatacag tgatcgtttt
     3721 tgaccgtatc agggaagaca gtcgactgat gcctaatgca ccaagaggtg agatcattaa
     3781 ccggnnnnnn nnnnnnnnnn nnnngaaacg cttagccgta ctataatgac ctcattaacg
     3841 gttttcctga cactgttgat cctgttcctt gtaggcggtg aagtaacccg tggatttgca
     3901 ttcgcaatgc tggtgggtgt aattaccggt atctattcat ctgtatttgt agccgcacct
     3961 atcttagtag atcttggcgg aaaaagatca ctcggaaaga aaacaatcga aaagactgta
     4021 aaaactgaag aagccgctaa accctgataa ctgattaact attcaataca aacctgcagt
     4081 atattactgc aggttttttt atgcgaaaaa atatttcgat attaaatgca ggcatttcct
     4141 tgttttgcct tttttttatt gtcaggatat tagtatc
//
LOCUS       DAORLS010000268         4168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11632_length_4168_cov_1.955021, whole genome shotgun sequence.
ACCESSION   DAORLS010000268 DAORLS010000000
VERSION     DAORLS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11632_length_4168_cov_1.955021"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>444
                     /locus_tag="PK977_16010"
     CDS             <1..>444
                     /locus_tag="PK977_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19680.1"
                     /translation="QDEEIIEHAQEINRILSFFLIYGFWYVLKLQKEKADLAQLYPRL
                     QEWKEYYTRPANPDEVTDKERNHLVHPSDEEWEKHKQEENAILQQIHQQEEAQRQQFY
                     DIVQPVIFRLMPDLLNIEGDCWTIYAVKLRDNYEQWKSFAGRLDTI"
     assembly_gap    445..535
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            617..1012
                     /locus_tag="PK977_16015"
     CDS             617..1012
                     /locus_tag="PK977_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRF19681.1"
                     /translation="MLYRLFFLLLLCPLVSACPVATEEPPDELSGKVISIKDGDTIAI
                     LYNNKTLTIRLAHIDCPEKKQPYGQAAKQFMSDKCFGQTVTIQHQHEYDRSKRLIGEV
                     ITSSGENLNKALVKAGLAWQYKKYSTNTI"
     gene            1209..>1687
                     /locus_tag="PK977_16020"
     CDS             1209..>1687
                     /locus_tag="PK977_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19682.1"
                     /translation="MMRLIGLCLGLCCIFQPAPAQELKRILPVGHSAPPFFAAASKDG
                     KRFVTLADDNTIKIWDGYTGKLLTDTKAHADRIINKISISPDGQQLLTSSFDNTIKIW
                     DIQTGKLKLAITDTEGLAGEALFSPDGKRILSMSDNSVRIWDAVSGQPLGKLEGGHT"
     assembly_gap    1688..1747
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1748..>4168
                     /locus_tag="PK977_16025"
     CDS             <1748..>4168
                     /locus_tag="PK977_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF19683.1"
                     /translation="KDHTARLWNVATGKTVAVLSGHTDAVHRAFFSPKGGKVVTLSVD
                     RTVKLWDRYSGTLLYSSPVMEKPVKYAALSPDEKTLFIAQDPLAGKVFNLDNGQELYE
                     LKGYGSAVDQLLFYPDGKKMVSVAGDSVAIIRDALTGNALQVLKGHQKRITGLQYSPD
                     FKKIITFSADNSIRLWNAATGELHSVLRGHSSEIRYSNFSADGRQLLTASSGNDYAVK
                     VWDIYSGRLLYDLNGHTKPVILSRYSPDGKLILTTRDSSPELWETASGQLLLQTEGHT
                     GTISTALFSPDSRRLLTASWDKTAAAWDAVTGKRLLHFTEHRDAVNTAGFSPDGKKVV
                     SASLDKNVRVWDAASGALLVKCHNSKRLSAIAFSPNGKTIAGLSVNDTLQLWDATTGA
                     LLGERFIRDKPAEHLVNYRNELHFTPDGQFIITGRGHPSIRVWNAVTLEKLYDFKGDL
                     SEAEVIAVAPDSKKIAGYFQKGLLMTWDLSSGKQLTEPVYPQDEISALAFSPDSRLLA
                     IAYGTGTIETRDAENGRVQAVMRGHTDFTGSLQFSPDGKKLLSSSDDNTCKRWSTVTG
                     KEICSFFMVDSSDYFIQLPSQYYFSTTAAAKLLHYVTPDLRVISFEQLDVKYNRPDLV
                     LQAIGNTDTALIKSYRKAWEKRIKKLGIDTTQFRDGYSVPECDFADRDKIEYEQKNGK
                     LSLRIKGNDSTYKLDRFNVWVNESPLYGQRGINIRRKNSNKLDTVVTIKLSQGENRIE
                     TSITNVNGTESYRMPLYVNYTPAVKQKEMTRFIGIGIDQFKESRYNLQYSSKDIRDLA
                     VKLKEKYK"
BASE COUNT         1148 a          935 c          981 g          953 t
ORIGIN      
        1 caggatgaag aaataataga acatgcacaa gaaataaacc gcatactatc cttcttcctc
       61 atttatggtt tttggtatgt gctcaagctg caaaaagaaa aagccgatct cgcccagctg
      121 tatccccgcc tgcaggagtg gaaagaatat tatacccgtc ccgcaaaccc ggatgaggta
      181 acagacaaag aacgcaatca cctcgttcat ccgtccgacg aagagtggga aaaacacaaa
      241 caagaagaaa acgccatcct gcagcagata caccagcagg aagaagcaca gcggcagcag
      301 ttctatgata tagtgcagcc cgtcatcttc cgcctcatgc ccgacctgtt aaacatcgaa
      361 ggcgattgct ggaccatcta cgccgtcaag ctgcgggaca attatgagca gtggaaatca
      421 ttcgccggcc gcctcgacac catcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaca
      541 gacttatagc tttgttcata cctgctctgc aatgttgtaa gtttcgcatc tcgtttctga
      601 ttatttttat caccctatgc tttaccgcct gttcttcttg ctccttcttt gtcccttggt
      661 atcggcctgt cccgttgcca cagaagagcc gcctgatgag ctcagcggta aagtgatcag
      721 cataaaagac ggcgatacca ttgctatttt atacaacaac aaaacactca ccatccgcct
      781 tgcacatatt gattgccccg aaaagaaaca accctacggg caggccgcca aacaatttat
      841 gtccgataaa tgcttcgggc aaaccgtcac aattcagcat caacacgaat acgaccgcag
      901 caaacgactg atcggtgaag tgatcacttc ctccggtgaa aacctgaaca aggcattggt
      961 aaaagcagga ttggcctggc aatataaaaa gtattcaacc aataccattt aatcagcact
     1021 ggaacaggaa gcaaggaagc aacgcatcgg cctctggagt gagccgaacc ccatagcgcc
     1081 atgggagtgg agagggaggt agttggaagc agatggcccg aacttatttt tctaatacat
     1141 cagcagttga acgttctacc cattcacggg tattggcggt atgaaggggc tatactatct
     1201 taccagttat gatgcgattg attggtttat gcctcggtct ctgctgtatt tttcaaccgg
     1261 ccccggcgca ggaactgaaa aggatattgc ctgtcgggca ttcggcccct ccgttctttg
     1321 ctgccgccag taaggatggt aaacgctttg ttaccttggc tgatgacaat accattaaaa
     1381 tatgggacgg ctataccggt aaactcctca ccgatacaaa agctcatgcc gaccggataa
     1441 tcaataaaat atccatcagc cccgatggcc aacaactgtt gacctcttcc tttgataata
     1501 cgattaagat ttgggatata cagacgggaa agttgaagtt agccattacc gacacggaag
     1561 ggctggccgg agaggcgctg ttttccccgg acgggaagcg gatactgagt atgtcagaca
     1621 attcagtacg gatatgggat gccgttagcg ggcagccgtt gggtaaactg gaaggcgggc
     1681 ataccgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnncca aagatcatac ggcccgttta tggaatgtgg ccacaggcaa aacagtggct
     1801 gtcctttccg ggcataccga tgcagttcac cgggcatttt tttccccgaa aggaggaaaa
     1861 gtggtcaccc tttctgttga ccggacggta aaattatggg accgttatag cggtacactg
     1921 ctttacagca gtcctgtaat ggaaaagccg gtaaaatatg ctgccctttc acctgatgaa
     1981 aaaacactgt tcatcgcaca ggatccgctt gccggtaaag tctttaacct tgataatggt
     2041 caggaactgt acgaactaaa aggatatggc agtgctgttg accagttgct cttttatccc
     2101 gatgggaaaa agatggtaag cgttgccggc gattcggtgg ccattatacg ggatgccctt
     2161 accggcaatg ccttgcaggt attgaagggt catcaaaaaa ggatcaccgg gctgcagtac
     2221 tcaccggact tcaaaaagat tattacgttt tcagcagata acagtatccg tttatggaat
     2281 gcagctaccg gtgagcttca ttctgtgctc cgcggacata gcagcgaaat ccgctacagt
     2341 aatttttctg cagatggccg tcaattgctg acagcctcat ccggtaatga ttatgcagta
     2401 aaagtgtggg atatctacag cggccggctg ctgtatgacc tgaacggaca tacgaaaccg
     2461 gtcatcttat cccgctattc tccggacggg aaactgatac tgactacccg ggacagcagt
     2521 cctgaattat gggaaacagc cagtggccag ctgctgttgc aaacggaagg acacacgggt
     2581 actatcagta cggcattgtt ttctcctgac agccgccgtc tacttactgc ttcatgggat
     2641 aagacggctg ctgcctggga tgccgtaacc gggaagaggc tcctgcattt tacggaacac
     2701 agggatgcag tgaatacggc aggattttca ccggacggta aaaaagtagt tagcgcctcc
     2761 cttgataaga acgtacgggt atgggatgca gccagcggag cattgctggt gaaatgccat
     2821 aacagcaaac ggctttctgc aatagctttt tcacccaacg gaaaaacgat cgcaggttta
     2881 tccgtaaacg acaccctgca attgtgggat gcaaccacgg gagcattact gggagagcgg
     2941 tttatccgtg ataagccggc ggaacatctt gtcaactatc gtaacgaact gcattttact
     3001 cccgatggtc aatttattat taccggaagg ggtcatcctt ctatccgggt atggaatgcg
     3061 gtgacactcg aaaaattata cgatttcaaa ggagacttat cagaagctga ggttattgcc
     3121 gttgcgccgg acagtaaaaa aattgccggt tattttcaga agggcctgct gatgacctgg
     3181 gatcttagtt ctggaaagca gttgacggaa ccagtttatc cccaggatga aatttctgcc
     3241 cttgcgtttt ctcctgacag ccgtttgctg gccattgctt acgggactgg tactattgaa
     3301 acaagagatg ctgaaaacgg ccgggtacag gcagtaatga gggggcatac ggattttacc
     3361 ggcagccttc agttctcccc ggatggcaaa aagctgctga gcagttctga tgataatacc
     3421 tgtaaacgat ggagcacagt aaccggtaaa gagatctgca gtttttttat ggtagacagc
     3481 agtgattatt tcattcagtt gccttcgcag tattacttct ccacaacagc agcggccaaa
     3541 ttgctgcact atgtgacccc cgacctgcgg gtgatcagtt ttgaacagct ggatgtgaaa
     3601 tataaccggc ctgacctggt gctgcaggct attggtaata ccgatactgc attaatcaag
     3661 agctaccgaa aagcatggga aaaaagaatc aagaaactgg gtattgatac cacgcagttc
     3721 cgggatggtt acagtgtgcc ggaatgtgat tttgctgaca gagataaaat tgaatacgaa
     3781 cagaagaacg gaaaattgtc gcttcgcata aaaggaaatg acagcacata caaactcgac
     3841 cgtttcaatg tttgggtaaa tgaatcacct ctttatggtc aaagaggcat caacatccga
     3901 agaaagaaca gtaataaact ggataccgtt gttaccatca aactttcgca gggagaaaac
     3961 cggattgaga ccagtattac caatgtaaac ggtaccgaga gctaccgtat gcctttgtat
     4021 gtcaactata ccccggcagt gaagcagaag gagatgacaa gatttatagg tataggtatt
     4081 gaccagttca aagaaagccg gtacaacctg caatacagca gcaaggatat ccgagacctg
     4141 gcggtgaagc tgaaagaaaa atacaaag
//
LOCUS       DAORLS010000269         4095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11905_length_4095_cov_1.518069, whole genome shotgun sequence.
ACCESSION   DAORLS010000269 DAORLS010000000
VERSION     DAORLS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11905_length_4095_cov_1.518069"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>291)
                     /locus_tag="PK977_16030"
     CDS             complement(<1..>291)
                     /locus_tag="PK977_16030"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRF19684.1"
                     /translation="EIPLWGPETPANDTIRKIAMKQLETMVANYYNHPSIFSWGVGNE
                     LRGRDADMKQFIRDMLQRCRELDPDRMTAYVSNTLTAGFSNNATFVPDAAAEG"
     assembly_gap    292..400
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<401..>699)
                     /locus_tag="PK977_16035"
     CDS             complement(<401..>699)
                     /locus_tag="PK977_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19685.1"
                     /translation="KLLPQWKNKEAIVQIALPKVNPWHFDHPNLYRVEVTIGTKKKLI
                     DRISAVTGFKELKFLNNQTYFNGERVKLMGVEWTAGSSPDYGFAEPDSVILRYCKL"
     assembly_gap    700..886
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<887..1405)
                     /locus_tag="PK977_16040"
     CDS             complement(<887..1405)
                     /locus_tag="PK977_16040"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19686.1"
                     /translation="MRIIFMIAGLLSSIAICAQRDTIHLNTGWQFATDKKAVGLQEKW
                     NETGLPISRLVQLPHTWNVEEENQNHYGWAWYQKEILVPAAWKNKRVLLLFGAVNHTC
                     IIYLNGKKIAENKGDGFSKFSVNMTDYLQIGKENRIIVAVNNDYGKQKVPFANSFDWP
                     NDGGIIRKVSLIV"
     gene            complement(1415..>2302)
                     /locus_tag="PK977_16045"
     CDS             complement(1415..>2302)
                     /locus_tag="PK977_16045"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRF19687.1"
                     /translation="NAFSAVAYQPDITSYDYDAPINENGEPTAKYFMLRNLIAAYTKE
                     PLPEIPQPVKTIEIPSFSLQYAGSIWDHLPKGIYSPQPQTMEAYDQNQGLIVYKTRLI
                     GHKSGKLKIWEPHDYALVYLNGKFIDTVFRDGGKWEVDLPKSEVKEPVLEILVEGMGH
                     INFAQFMIDRKGITDRVTLNGMTLMNWETFLLPMDEKFIASLKLEKTDNINKKSCQFF
                     KGEFTLEEKGDTFFDMSRYSKGVLYVNGHNLGRFWNIAPQQRLYCPSSFLKKGKNEVI
                     VFDLHQELAESLIGHSTMY"
     assembly_gap    2303..2328
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2329..3235)
                     /locus_tag="PK977_16050"
     CDS             complement(<2329..3235)
                     /locus_tag="PK977_16050"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRF19688.1"
                     /translation="MKYLIILLTLVCITASVFPQQKHEFALGKEVFLLDEKPFQIISG
                     EIHPARIPAEYWRHRIKMIKAMGCNTVAAYIFWNYHEVKQGEFDFKTGNRNIAEFIRI
                     CQQEGMWVLLRPGPYVCAEWDFGGLPAYLLKIPDIRIRCMDPRYMTAVTRYLKQLAKE
                     TVPLQCIKGGPILMVQVENEYGSYGNDREYMKALQQLLKNSGFNVPLYTADGPTPYML
                     EAGSLPDAAIGLDSGGDEKDFEQARKHNPNVPAFSSETYPGWLTHWGEKWARPDTGRL
                     KKEVEYLLKNNKSFNFYVIHGGTNFG"
     assembly_gap    3259..3440
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3441..>4095)
                     /locus_tag="PK977_16055"
     CDS             complement(<3441..>4095)
                     /locus_tag="PK977_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRF19689.1"
                     /translation="RTEKRNYDSYLLFMKNQLSELLSNYGEIMSIWLDGHWDQTNPEG
                     SADMSSRVNWQYEELYTLIHRLQPQCMIGNNHHLDPMPGEDFQMFEKDLPGQNKSGLS
                     YQAASEELPLETCETMNNSWGYNITDNNYKSVKSLIHYLVNAAGLNTNFLLNVGPMAN
                     GKIQPEFLDTLKAAGEWMKQYGESIYGTRGNIIPAQEWGVITQKNKTLYIHILKMSAV
                     "
BASE COUNT          961 a          814 c          649 g         1167 t
ORIGIN      
        1 ccttctgcag cagcatcagg cacaaaagtg gcattattgc taaaccctgc tgttaatgtg
       61 ttactaacat aagcagtcat cctgtccggg tcgagttcac ggcagcgttg cagcatatct
      121 cgtatgaatt gtttcatgtc cgcatccctt ccccgcaact catttcctac tccccaggag
      181 aaaatagagg ggtggttgta ataatttgcc accattgttt ccagttgttt catggcaatc
      241 ttacggatag tatcgtttgc cggggtttcc ggaccccaca atggtatttc cnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agtttgcaat agcgaagaat
      421 tactgaatca ggttctgcaa atccataatc agggcttgag cctgctgtcc attctactcc
      481 cattagtttc acccgctccc cattgaaata ggtttgatta tttaaaaatt taagttcctt
      541 aaaacctgtc actgccgata tcctgtcaat aagttttttc ttagtaccta ttgtaacttc
      601 tacccggtac aggttgggat gatcaaaatg ccagggattc actttgggta aggcaatctg
      661 tacaatagct tctttgtttt tccattgtgg cagcagtttn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaact attaaggaaa
      901 ctttgcgaat aataccacca tcatttggcc aatcaaaaga gttagcaaaa ggaactttct
      961 gttttccata atcattattc actgccacaa tgatgcgatt ctctttaccg atttgcagat
     1021 aatctgtcat gttaactgaa aacttactaa atccatcacc tttgttctcg gcgatttttt
     1081 taccattaag gtaaataatg caggtatggt tcactgcccc aaacaacagc aacacccttt
     1141 tgtttttcca tgctgccggt acaaggattt ccttctggta ccatgcccag ccataatggt
     1201 tttgattttc ttcttctaca ttccaggtat gcggaagttg tactagccgg gaaattggta
     1261 aacccgtttc attccatttt tcctgtaaac ccacagcctt tttatctgtt gcaaactgcc
     1321 agccagtatt caagtggata gtatctcttt gcgcacagat cgctattgaa gaaagcaatc
     1381 ctgcaatcat aaatattatt ctcataaaga acctttagta cattgtactg tgaccaatta
     1441 atgattctgc taattcctgg tggaggtcaa aaacaattac ttcattcttt cctttcttta
     1501 agaaagacga aggacaatac agcctttgct gcggagcaat attccaaaac cggcccaggt
     1561 tatgaccgtt tacatataat acccccttgg aataacgact catatcaaaa aaggtatctc
     1621 ctttctcttc taaagtaaat tcccctttaa aaaactggca actcttttta ttaatattat
     1681 ctgttttctc cagctttaat gatgcaatga atttttcatc catcggcaag agaaaggttt
     1741 cccaattcat caatgtcata ccattcaggg ttactctgtc tgtaatgcct ttacgatcaa
     1801 tcatgaattg tgcaaagttg atatggccca tcccctcaac cagtatttcc agaacaggct
     1861 ctttaacttc tgacttaggg agatctactt cccatttacc gccatcccga aaaactgtgt
     1921 ctataaattt tccgttcaga tacacaaggg cataatcatg tggttcccat atttttagct
     1981 taccgctttt gtgtcctata agtcttgttt tataaactat caagccctgg ttttgatcgt
     2041 acgcttccat ggtttggggc tgtggcgaat aaataccctt gggtaagtga tcccagatac
     2101 tcccggcata ttgcaaggaa aaagaaggta tctcaatcgt ctttaccggc tgaggaattt
     2161 cgggcagggg ttcctttgta taagctgcaa tcagatttcg caacataaaa tacttcgctg
     2221 ttggttcccc attctcattg attggcgcat cataatcata actggtaata tcgggctgat
     2281 aagctacagc tgaaaatgca ttnnnnnnnn nnnnnnnnnn nnnnnnnnag ccaaaatttg
     2341 tgcctccgtg tatcacataa aaattaaatg acttattatt ttttagcagg tattctactt
     2401 ctttttttaa cctcccggta tcaggtcttg cccatttttc gccccagtgt gttagccagc
     2461 caggataggt ttctgaacta aatgccggta cattgggatt gtgctttcgg gcctgttcaa
     2521 aatccttttc gtcaccaccg ctgtcaagtc caatggcagc atctggcaaa cttcctgctt
     2581 caagcatata aggagtaggt ccgtctgctg tgtaaagggg cacattgaaa ccactgtttt
     2641 ttaacaactg ctgcaatgct ttcatgtatt ccctgtcatt tccataactg ccatattcat
     2701 tttctacctg taccattagt ataggacctc ctttgataca ttgcaggggt accgtttctt
     2761 tggccagttg ctttaaataa cgggtaactg ctgtcatgta cctgggatcc atacaccgta
     2821 tgcggatgtc cggtattttt aataagtagg cgggtaatcc gccaaaatcc cattcagcac
     2881 aaacataagg tccgggtctc agtaataccc acataccttc ctgctggcat atacgaataa
     2941 actcagctat gtttctattc cctgttttaa aatcaaattc accttgtttg acttcatgat
     3001 aattccagaa aatataagca gcaaccgtat tgcatcccat tgcctttatc atctttatcc
     3061 tgtgacgcca atattcagca ggtattctcg caggatgtat ttcaccactg ataatctgaa
     3121 aaggcttttc atccagcaga aaaacttctt tgcctaatgc aaattcatgt ttttgctgcg
     3181 ggaatactga ggcagtaatg caaacaagag ttaataaaat aattaaatat ttcatgctga
     3241 ttaaaatact attgttaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn aaccgctgac attttcagaa tgtgtatata taatgttttg
     3481 tttttttgtg ttatcacgcc ccattcctgt gccgggataa tattgccccg ggtgccgtaa
     3541 atactttcgc catattgctt catccattca cccgctgctt ttaatgtatc aaggaattcc
     3601 ggttgtattt tcccatttgc catcgggccc acattcaaca aaaagttagt gttcagcccc
     3661 gcagcattta cgagataatg aataagagac tttaccgact tgtaattatt atcagtaata
     3721 ttatatcccc aggaattatt catggtttcg caggtttcga gtggcaattc ttctgaagca
     3781 gcctggtagc tcagtcccga tttgttctga cctggtagat ctttttcgaa catctgaaaa
     3841 tcttcgcccg gcataggatc gaggtgatgg ttattgccga tcatacactg cggttgcagg
     3901 cgatgaataa gcgtatacaa ttcttcatac tgccaattga ctcgggatga catatctgca
     3961 cttccctccg gatttgtctg gtcccaatga ccatcgagcc aaatggacat gatttcgcca
     4021 tagtttgaaa gcaactcgct caattggttc ttcatgaata gcaggtaact atcataattc
     4081 ctcttttcag ttctt
//
LOCUS       DAORLS010000270         4087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11936_length_4087_cov_1.421379, whole genome shotgun sequence.
ACCESSION   DAORLS010000270 DAORLS010000000
VERSION     DAORLS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_4087_cov_1.421379"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PK977_16060"
     CDS             complement(<1..358)
                     /locus_tag="PK977_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19690.1"
                     /translation="MATNVAGEIEKEYGSLSVDIPTIRFEFNIIGIAEKLSMLQKIYM
                     AASTVFLFGLLFYINPPVIFSALLSLIILSVLFYNYYSLFDKVIIDFNHKEITLRNKF
                     RIVTGIRKKIGKTVTLP"
     gene            complement(360..>488)
                     /locus_tag="PK977_16065"
     CDS             complement(360..>488)
                     /locus_tag="PK977_16065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRF19691.1"
                     /translation="DGGVTLENAPSIIAAGADVLVAGNTVFKSADPVGTIHSLKSI"
     assembly_gap    489..579
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<580..1016)
                     /locus_tag="PK977_16070"
     CDS             complement(<580..1016)
                     /locus_tag="PK977_16070"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRF19692.1"
                     /translation="MPIIAPSLLSANFLNLDADCKMLNESEADWYHLDVMDGRFVPNI
                     SFGPMIIEFFRKATTKVCDVHLMIEEPEKYAEQFKNAGADILSVHIEACRHLHRNIQQ
                     IKSLGMKAGVAVNPHTPVSSLSDILHDIDLVCLMSVNPGFGGQ"
     gene            complement(1061..2062)
                     /locus_tag="PK977_16075"
     CDS             complement(1061..2062)
                     /locus_tag="PK977_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF19693.1"
                     /translation="MNKFAKIAVPLAILLIFISGCTDKSTNSPYGELLSQPPYASLTD
                     SIKKQPDNDELYFRRAVLLNTNELPEPSLDDFKKAWNLKKDERYAFGISNLLLDRKPD
                     SAILFLRVALVELPASTLLQLTLARCYESQNNNDEALTLCNSILEKNPQQVDVLKMKA
                     TLLDKKGNGSEAISILEKAYAITPYDAELNYELAYKYAEAKNIKVLSLCDSLIQKDSL
                     KIHAEPYYYKGIYYSNIGNKAAALNQFNEAIQHDYYYLNAYIEKGRIYYDQKKYAEAL
                     KVFELANTISATFPDAYYWMGRSQEAMGQKTDAKLNYQRAYGLDKSFTEAKEAANRL"
     assembly_gap    2133..2173
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2190..>2483
                     /locus_tag="PK977_16080"
     CDS             2190..>2483
                     /locus_tag="PK977_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19694.1"
                     /translation="MKKLVFPILIVSIILLACSGGSKTTGSVVEADTLRYPEETHLKN
                     IQQLTFGGDNAEAYWSYDGKYLVFQRTNSKEGVNCDQIFVGKVPTKKGEKFTYK"
     assembly_gap    2484..2590
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2591..>2961
                     /locus_tag="PK977_16085"
     CDS             <2591..>2961
                     /locus_tag="PK977_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19695.1"
                     /translation="CPPVPDRAKYGNKYIWPLYSSYDIFMADLDGKIVKQLTSSKYYD
                     AEATLSPDGKKMIYTSTKDGDIELYIMDLETGAEKRITHTLGYDGGAWFSPDGKKLIW
                     RASRPKTEAEVKEYKDLLAEN"
     assembly_gap    2962..2989
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2990..3314
                     /locus_tag="PK977_16090"
     CDS             <2990..3314
                     /locus_tag="PK977_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19696.1"
                     /translation="NMEVWVANADGSNARQVTSYGQANWAPTYMPDSKRIIFASNHEY
                     KRGFPFNLYTINEDGTNLQKVSRDKGFDAFPMFSPNGKKIVFSSNRNNGGNRDTNVFI
                     ADWEE"
     gene            3526..3834
                     /locus_tag="PK977_16095"
     CDS             3526..3834
                     /locus_tag="PK977_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19697.1"
                     /translation="MLLVGLYQWISGSAGLKAIQNYGMGDVMKTPALRFFAVEHFTAM
                     LLAIILVHLGYSYGKKSVPDAQKHKRILWFFGVALLLILISIPWPFRENIGRPLFPGM
                     "
     gene            complement(3878..>4087)
                     /locus_tag="PK977_16100"
     CDS             complement(3878..>4087)
                     /locus_tag="PK977_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRF19698.1"
                     /translation="KQLAIHQPQLLVCGHSHILKIMYDSQINCLYMNPGAAGKQGWHK
                     MRTLIRFSIDGSDIRNCEVIELGKR"
BASE COUNT         1091 a          734 c          850 g         1145 t
ORIGIN      
        1 aaggaagggt tacggttttg ccgatttttt ttcggatacc ggttacaatt ctaaatttat
       61 tacggagcgt aatttccttg tgattaaagt ctatgatcac cttgtcgaac aaagaataat
      121 aattgtaaaa cagaactgac aggattatca atgagaggag ggctgaaaaa attacaggtg
      181 ggttaatata gaaaagcaag ccaaaaagaa aaacggtact tgcagccata taaattttct
      241 gaagcattga tagcttttca gctatcccga taatgttgaa ttcaaatcta atagttggaa
      301 tgtcaaccga taaagagcca tattcttttt caatttcacc agctacatta gtggccacgt
      361 taaatggatt ttaatgagtg tatggtacca acaggatctg ctgatttgaa caccgtattc
      421 ccggctacca gtacatcagc gccggctgct ataatggaag gagcattctc taaagtgaca
      481 ccgccatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tctgccctcc aaaacccggg
      601 ttaacactca tcaaacaaac aaggtctatg tcatgaagta tatcgcttaa agaggaaacc
      661 ggtgtgtgcg ggttcacggc caccccggct ttcatgccaa gtgattttat ctgctggata
      721 ttccggtgca ggtgacgaca ggcttcaata tgaacggata gtatgtcagc cccggcattt
      781 ttgaattgct cggcatattt ctccggctct tcaatcataa ggtgtacatc gcaaaccttg
      841 gtagtggctt tgcggaaaaa ctcaatgatc atggggccaa aactgatatt gggtacaaat
      901 cggccgtcca ttacgtctag atgataccag tctgcttcgc tttcattcag cattttgcag
      961 tcggcatcaa gattcaggaa gttggcagat aaaagggaag gggcaatgat cggcattgga
     1021 gtgtatttgc gctgcaagat acgttttgtg cggtaagatt ttaaagcctg ttggctgcct
     1081 cttttgcttc tgtaaaactc ttgtccagcc cataagccct ctggtaattc agtttggcat
     1141 ctgttttttg gcccatggct tcctggcttc gtcccatcca gtagtaagca tcaggaaaag
     1201 tggcggaaat agtattggca agttcaaata cttttaaggc ttcagcatat tttttctggt
     1261 cataatagat ccttcctttt tcaatatagg cgttcaggta atagtaatca tgttgtatgg
     1321 cctcattgaa ctggttcaat gccgcagctt tattgcctat attggagtaa taaatgcctt
     1381 tataataata aggttctgcg tgaatcttta gcgagtcttt ttgaattaat gagtcacaaa
     1441 ggctcagcac tttaatattt tttgcttccg catatttata agccagttca tagttcagtt
     1501 cggcgtcata aggtgtgata gcatacgctt tttcaaggat gctgatcgct tcgctgccgt
     1561 ttcctttttt atcgagcagg gtagccttca ttttgagcac atcaacttgc tgagggtttt
     1621 tttccagaat gctattacac aatgttaggg cttcgtcatt attattttgt gattcatagc
     1681 aacgggccag tgttaattgt aaaagagtac tggcgggcaa ttccactaaa gctacccgga
     1741 gaaagagtat ggcagagtct ggtttccggt caagcagcag gttgcttatt ccaaaggcat
     1801 agcgttcatc cttttttaag ttccatgctt ttttgaaatc atctaaagat ggttcgggca
     1861 gttcattagt atttaataat acggcccttc tgaaatataa ttcatcgtta tctggctgtt
     1921 tttttatgct gtcagtcagg ctggcatagg gtggttgtga aaggagttca ccataagggg
     1981 aattcgtgga tttatcagta caaccgctga taaagatcag taaaatggca aggggcactg
     2041 ctatttttgc aaacttgttc atgattcaaa actaaccgtt ttttttcgcc gaggtatgac
     2101 aattagctga caatacaggc tgctgttgaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnngttaaaa cgaatgaaaa tgaaaaaact tgtattcccc atactgattg
     2221 tttcgatcat attgctggcc tgttccggag gctctaaaac aactggtagt gtcgttgaag
     2281 cagacaccct gcgttacccg gaagaaacac atctgaagaa tatacagcaa ctgacctttg
     2341 gcggcgataa tgcagaagcc tattggagct atgatggtaa gtatctcgtt tttcagcgca
     2401 ctaattccaa agagggtgta aactgcgacc agatctttgt tggaaaagtt cccacaaaga
     2461 agggggagaa gtttacttat aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn atgtcccccg gttccggaca gggcaaaata tggcaataaa tatatatggc
     2641 ccctgtacag cagttatgat attttcatgg ctgacctcga tggcaaaatc gtaaaacaac
     2701 tcaccagttc taaatattat gatgcggagg ctactttgtc acccgatggg aagaagatga
     2761 tctacaccag cacaaaggat ggtgatattg aattgtacat tatggacctg gaaacaggtg
     2821 cagaaaaacg tatcacccat acgttgggat atgacggtgg tgcatggttt agcccggatg
     2881 gaaaaaaatt aatctggaga gcctcccggc caaaaaccga ggccgaggta aaagaataca
     2941 aagatttact ggcagaaaat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aatatggaag
     3001 tgtgggtagc caatgcagat ggaagtaatg caagacaagt gacttcatat ggccaggcta
     3061 actgggcacc tacgtatatg ccggacagca aacgcatcat ttttgcttct aatcatgaat
     3121 acaaaagagg gtttccattt aatctttata cgataaatga agatggtacc aacctgcaga
     3181 aggtgagccg tgataaaggg tttgatgcct tcccgatgtt ttcgccgaat ggcaagaaga
     3241 ttgttttttc cagtaaccgc aataacggcg gcaaccgcga tacgaatgtt tttatagcgg
     3301 attgggaaga ataaacagta ttttaaattt cagcccgaag cctttagttt tgttacaaac
     3361 aaattgctta tgtattccgg aatgcttcat ttacataatt tccttaggtg gataatcctg
     3421 atattgttgt tggtcgctat ctacaaacat tttgttgacc ggcataaacc atttactgca
     3481 ggacacaaaa agattgatct tttccttttg ataagttgtg acattatgct gcttgtcgga
     3541 ttgtaccaat ggataagtgg cagcgccgga ttaaaggcaa ttcagaatta tggtatgggt
     3601 gatgtaatga aaacaccggc tttgcgattt tttgcggtag aacattttac tgcaatgttg
     3661 ctggcgatta ttttggtgca tttgggatat tcttatggta aaaagtccgt acccgatgca
     3721 caaaagcata agcgaattct ttggttcttt ggagtggctt tactcctcat actgatttcc
     3781 attccatggc ctttcaggga gaatattgga aggcccctgt ttccggggat gtaaaatttt
     3841 agcaaagaag gcgctggcat gaaaatgtca gccttcttta tcttttaccc aattcaatca
     3901 cttcacagtt acgaatatca gacccatcaa tggaaaaccg gatcagcgtc cgcattttgt
     3961 gccagccttg tttacctgca gcgccggggt tcatgtacaa acaatttatt tgactgtcat
     4021 acatgatttt taaaatgtgt gaatgaccac acaccagtaa ctgtggctga tgaatagcca
     4081 gttgttt
//
LOCUS       DAORLS010000271         4081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_11964_length_4081_cov_1.397417, whole genome shotgun sequence.
ACCESSION   DAORLS010000271 DAORLS010000000
VERSION     DAORLS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11964_length_4081_cov_1.397417"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>237)
                     /locus_tag="PK977_16105"
     CDS             complement(<1..>237)
                     /locus_tag="PK977_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF19699.1"
                     /translation="GANSASTDINLVRTRAGLPNTTAGTQTQILAAIEKERVTELFYE
                     GHRWYDLKRTGQLQAVLGAIPYKAANYKPHYNLWP"
     assembly_gap    238..260
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<261..>639)
                     /locus_tag="PK977_16110"
     CDS             complement(<261..>639)
                     /locus_tag="PK977_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF19700.1"
                     /translation="TGVNNVFKRGSREAIFSMGSTGTGLLFDNRAVLGWLTLPATSGN
                     TTSSHCAIPAAMLARFESGDQRNVNGNWTISLFGKVFANKYLYNSSATSTSINANPQD
                     FIYQRLAELYLIRAEARAQQGNLT"
     assembly_gap    640..649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<650..>1314)
                     /locus_tag="PK977_16115"
     CDS             complement(<650..>1314)
                     /locus_tag="PK977_16115"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF19701.1"
                     /translation="LEVPLPIDQLSTGNVLNTKPTIISAVNGMYSAFSEGIIKANYYK
                     ITFWWSDEGEISPLPGSEIGDIISANIVPANTQLMQWVFFYRTIFRANNLIEKLPSVS
                     TTILPDAEKKQYIAAAKYIRAAEHFTLVSSWGDIPLITTTSADENLNKPRTPANEVYN
                     QIIKDLQEAAADLPSTVSTSNSKTIHNKFQPLALLAKVYLYLGRWAEAEATASEVINS
                     SQY"
     assembly_gap    1315..1338
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1426..>1740)
                     /locus_tag="PK977_16120"
     CDS             complement(1426..>1740)
                     /locus_tag="PK977_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19702.1"
                     /translation="TYKYPRLFSGAASNATTNLLSTIFPLSSAALNDVFYARLKNLSL
                     SYTLPTAFVSKAKINKAVVYLRGQNLFTWTSDRIYKDPELVQIRGGQVLKTWTAGIQL
                     SL"
     assembly_gap    1741..1765
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1766..>2367)
                     /locus_tag="PK977_16125"
     CDS             complement(<1766..>2367)
                     /locus_tag="PK977_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF19703.1"
                     /translation="KDRILLNINWYRTRSTDLLASTPIPSQTGFNSYITNIPAVVENK
                     GWEFELNTQNTRQKAKLQWKTNFNLTLLKNKLIEFPGLERSSFANRLKIGLPVNSPRT
                     LLNTEWTQIFERVDPATGLPVFKDLNGDGLINNNDRTFIGSAIPRTFGGFGNQLSYKG
                     FELDVFFQFSQQLATNWLFNTTYMGQLNNPPIADFYGNYW"
     assembly_gap    2368..2415
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2416..>2765)
                     /locus_tag="PK977_16130"
     CDS             complement(<2416..>2765)
                     /locus_tag="PK977_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF19704.1"
                     /translation="NSAFARVNYNWENRYLINLTYRRDGSSRFGPKKQWGNFGALGLG
                     WIFTSENWLKDKLSFLSYGKLRGSYGTSGNDNITDYQYTSLYSSNFYDGSAGLAANYL
                     ADSSIGWENSRKLD"
     assembly_gap    2766..2781
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2782..>3730)
                     /locus_tag="PK977_16135"
     CDS             complement(<2782..>3730)
                     /locus_tag="PK977_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19705.1"
                     /translation="ERLTLAAYRQMRRDAFNMGNHNPTTGVAINPITPTFNNAPDLLV
                     WDSTNATADWTDFEYGNSEPSINAQANLSGGEKRLNFYASGGYQKQNDITRGSPSLER
                     ISGNLNVNHTSLNDKLRISLNTSYITTKLKPSRGGGSGGLLASAPPNMPMKNNDGSIW
                     WPSPTITQSSLLISPLAMEEARTESKTKNLIANMEVTYRIYKGLQVKTLLGYNDQITT
                     TETGTPSTSINPLNPGTNVPNLNWAQSKFQSLNFEPQLLYSGKLSKGKIEALVGSTFF
                     DRKTSSNSINLNGYTSDLLLYSWAAATSVANRSNTSTYYR"
     assembly_gap    3731..3872
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3873..>4081)
                     /locus_tag="PK977_16140"
     CDS             complement(<3873..>4081)
                     /locus_tag="PK977_16140"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF19706.1"
                     /translation="SGTEIQIRGINTIQAGNQPLVIVDGAILPDPNRGLGTAIGSYMS
                     FGSTNMTAINPADIESIEILKDADAT"
BASE COUNT         1025 a          728 c          785 g         1255 t
ORIGIN      
        1 aggccacaaa ttataatgcg gtttgtaatt agcggctttg tatggtatag ctcctaaaac
       61 tgcctgcaat tgaccggtac gcttaaggtc ataccagcgg tgaccttcgt aaaataattc
      121 tgtcactctt tctttttcta ttgctgcaag tatttgtgtt tgtgtgccgg cagtggtgtt
      181 gggtagccct gctcttgtac gaaccaggtt aatgtcagtt gaagctgaat ttgcaccnnn
      241 nnnnnnnnnn nnnnnnnnnn agttagatta ccttgttgtg ccctggcttc tgcccttata
      301 aggtacagtt cggctaatcg ttgatagata aaatcctggg gattggcatt tatacttgtt
      361 gaagtagcag aagagttata caagtattta ttggcaaata cctttccaaa tagtgaaata
      421 gtccagttgc catttacatt cctttggtct ccggattcaa acctggctaa catagcggct
      481 ggaatagcac aatggctgga agtcgtattg ccacttgtgg caggaagtgt aagccatccc
      541 aatacagccc tgttatcaaa caacaagcct gtaccagttg aacccattga aaaaatggct
      601 tcccggctgc ctcttttaaa tacattatta acccccgttn nnnnnnnnna tactggctgc
      661 tatttattac ttccgatgca gtagcttctg cttcggccca tcttcctaag tatagataca
      721 ctttggcaag taaagcgagt ggctgaaatt tattatgtat cgttttagag ttgctcgtac
      781 taacagttga aggcaaatct gcagcagctt cctgtaagtc ttttataatt tgattgtata
      841 cttcatttgc aggagttctt ggtttattaa gattttcatc cgcactggtt gtggtaatta
      901 atggtatatc tccccaagaa gataccagtg taaaatgttc tgctgctctt atatatttgg
      961 cagcagcaat gtattgtttc ttttcggcat caggtagtat agtggtactt actgaaggca
     1021 atttttcaat taaattattt gccctgaata ttgtcctgta aaagaatacc cattgcatca
     1081 gttgtgtatt tgcaggcaca atattagcgc tgatgatatc cccgatttcc gaacccggta
     1141 agggactaat ttctccttca tctgaccacc agaaagtgat tttgtaataa ttggctttta
     1201 ttataccttc tgaaaaggca ctgtacatgc cattaacggc tgaaataatc gtgggtttag
     1261 tattaagaac atttcctgta cttaattgat caattggtaa tggaacttcc agatnnnnnn
     1321 nnnnnnnnnn nnnnnnnncc gtaattgtcg gggctataca aataactaat agtaaaagcc
     1381 ttgtgggtat tttatataag aatttcataa taaatttctt ttattttata atgataattg
     1441 aatgcctgct gtccaggttt ttaatacctg gccacctcta atttgtacaa gctcaggatc
     1501 tttatagatc ctgtcactgg tccatgtaaa aaggttttgt ccccttaaat acacaacagc
     1561 tttattaatt tttgctttag aaacaaatgc tgttggtaaa gtataggaaa gagacaggtt
     1621 ctttaaccgg gcatagaaaa catcatttaa tgctgcagat gaaagaggga aaatagtgct
     1681 taagagatta gttgtagcat tagaagctgc accactgaac aatctcggat atttatatgt
     1741 nnnnnnnnnn nnnnnnnnnn nnnnntccag taattgccat aaaaatcagc aatgggtgga
     1801 ttattcaatt gacccatata tgtggtgtta aacaaccagt tggtagccag ttgctgtgag
     1861 aattggaaga aaacatccag ttcaaatcct ttatatgata gctgattgcc aaagccgcca
     1921 aaagttcttg ggatggctga gccaatgaag gtgcgatcat tattgttaat cagtccgtct
     1981 ccgttcaagt ctttaaaaac aggtaatccg gtagcagggt ccaccctttc aaatatttgc
     2041 gtccattctg tattcaataa tgttctgggg ctattaacgg gtaaaccaat ctttaacctg
     2101 tttgcaaatg atgaccgttc aaggcccgga aattctatga gtttgttttt tagtaaagtg
     2161 agattaaaat tagttttcca ttggagtttt gctttttgcc ttgtgttttg agtgtttaac
     2221 tcaaactccc atcctttgtt ttcaacaact gccggtatgt tcgttatgta actattaaaa
     2281 ccggtttgtg aaggaatagg agtgctggca agcaaatcgg tagaacgggt acggtaccag
     2341 ttaatattta gcaaaattct gtccttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnatcca gttttcggct gttttcccaa ccgatagatg agtcggccag
     2461 gtagtttgct gctagacctg ctgatccatc ataaaaattt gaagaataca aactggtgta
     2521 ttggtagtcg gtaatattat cattaccact ggtgccataa ctgcccctta gtttgccata
     2581 gcttaggaat gaaagtttgt ctttcaacca gttttcactg gtaaaaatcc atccaagacc
     2641 caaagctcca aaattacccc attgtttttt ggggccaaag cgggatgatc cgtctcttcg
     2701 gtaagtaaga ttgatcaggt acctgttttc ccaattataa ttaactctgg caaatgctga
     2761 attaannnnn nnnnnnnnnn natctataat aggtagatgt gttggaacgg tttgctacag
     2821 atgtagctgc tgcccagcta tagagcaaca ggtctgatgt atatccgttt aaatttattg
     2881 aattactact tgtttttctg tcaaagaaag tactgccaac aagtgcttca attttccctt
     2941 ttgacagttt tccactgtat aaaagctgcg gttcaaaatt caaagactgg aatttgcttt
     3001 gagcccagtt aagattgggt acgttagtgc ccggattcag ggggttaatg gaagtagagg
     3061 gtgtacctgt ttcagttgtt gtaatctggt cattatatcc caaaagtgtt ttaacctgca
     3121 accctttata aatgcggtag gttacctcca tgttggcaat taaatttttt gttttgcttt
     3181 ccgttcttgc ttcctccatg gcaaggggac taattaacaa actgctttgt gtaatggtcg
     3241 ggctgggcca ccagatactg ccatcattgt ttttcattgg catatttggc ggagcactgg
     3301 ctaacaatcc tccggaacca ccgcccctgg atggtttaag tttagtggtg atgtatgaag
     3361 tatttaagga aattctcagc ttgtcattaa gtgaagtatg atttacattt aagttacccg
     3421 aaattctttc cagtgaaggg cttcctcttg taatatcatt ttgtttctga tatccgccac
     3481 ttgcataaaa atttaatctt ttttcacctc ctgacagatt ggcctgcgca ttaatggatg
     3541 gttctgaatt accgtattca aaatcggtcc agtcagcagt ggcattggta gaatcccaaa
     3601 ccagcaggtc aggtgcatta ttgaaggtag gcgttattgg gttgatcgca accccggtcg
     3661 taggattatg attgcccatg ttaaatgcat cacggcgcat ttgtctgtag gctgccagcg
     3721 taagtctttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtggcatc tgcatctttt aagatctcaa
     3901 tactttcaat atctgcagga tttatggcgg tcatattcgt actgccgaat gacatataag
     3961 agcctatagc agtgccaaga cccctgtttg gatcggggag aattgcccca tcaactataa
     4021 ccagtggctg gttacctgcc tgtattgtat tgatacccct tatttgtatt tcagtgccgc
     4081 t
//
LOCUS       DAORLS010000272         4064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12023_length_4064_cov_2.068596, whole genome shotgun sequence.
ACCESSION   DAORLS010000272 DAORLS010000000
VERSION     DAORLS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12023_length_4064_cov_2.068596"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PK977_16145"
     CDS             complement(<1..545)
                     /locus_tag="PK977_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF19707.1"
                     /translation="MAARTDLFQSPDYFLVDELLTEEHKMIRDTVRSYVKKEISPIIE
                     DYAQKAEFPQHIVKQLGELGCFGPTVPVEYGGGGLDYISYGLMMQELERGDSGVRSTA
                     SVQGSLVMFPINAYGSEEQKKKYLPKLASGEWLGCFGLTEPNHGSDPASMLTNFKDAG
                     DHVILNGAKMWISNAPFAQVAV"
     gene            667..>859
                     /locus_tag="PK977_16150"
     CDS             667..>859
                     /locus_tag="PK977_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19708.1"
                     /translation="MKYALISLVFLFCCSVVTYAQKDSTKSGAEPRVKQFWMVILKTG
                     PKDKEITDSIQRKTLFAGHF"
     assembly_gap    860..869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <870..1097
                     /locus_tag="PK977_16155"
     CDS             <870..1097
                     /locus_tag="PK977_16155"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRF19709.1"
                     /translation="RLHKEGILKAAGPFGKNDFTWRGLFIMDCATKEEAENHVKTDPT
                     VAAGVFIYDIVPWWSEPSGSFKTGKPEKKEE"
     gene            1094..1528
                     /locus_tag="PK977_16160"
     CDS             1094..1528
                     /locus_tag="PK977_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HRF19710.1"
                     /translation="MSYPAKEIIRNFLSVVAGVMIALIIILPIGLFAGLLVFSDSNIP
                     KSKETLSKLLLIFGLIAGCLLGGYTTVKMSTRKDLVHAFITGIVLTFLYALVNDFEFD
                     WRYPDTGIAYAGFIPIVMIGGIIGERKKRTTSNSHSSPPGTP"
     gene            complement(1488..2291)
                     /gene="pyrF"
                     /locus_tag="PK977_16165"
     CDS             complement(1488..2291)
                     /gene="pyrF"
                     /locus_tag="PK977_16165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRF19711.1"
                     /translation="MTRLQLIEQIRKKKSYLCVGLDTDITKIPRHLSTDPDPVFAFNK
                     AIIDATKDLCVSYKINTAFYEALGVKGWEAMEKTVRYIGDEHFKIADAKRGDIGNTSD
                     QYAKAFFETLPFDAITVAPYMGEDSVKPFLQHKGKWAIVLGLTSNKGANDFELLHTGG
                     EFFYEKVLETVSAWGTEDNLMFVIGATREGELSNVRKLTPHHFYLVPGVGAQGGSLKD
                     VSEEAMIKDCGILVNISRAILYASAGQDFAEKAREVAKEYQEEMSGYLK"
     gene            complement(2369..3715)
                     /locus_tag="PK977_16170"
     CDS             complement(2369..3715)
                     /locus_tag="PK977_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF19712.1"
                     /translation="MKENITIIGAGLVGSLLSIYLSKRGYKVKVYERRGDMRKEKMSA
                     GRSINLALSDRGLLALEKVGLAEEIKKISIPMHGRYIHNLDGSSAFQPYGKEGQYINS
                     VSRGTLNMKLMDLAEEQGVEIYFNEKLSKIDWSNEVIEFESNKNKPLPSFKTDLIFGA
                     DGAFSAARLQHQLQHGKFNYSQYYIDCGYKELTIPPTSDGEFAMQVNALHIWPRKDYM
                     LIALPNLDKTFTCTLFFPFEGEASFEKLSTPEAVKSFFERTFPDAVKLMPDYINEFFT
                     NPTSSLVTVKCFPWIRGDKFALIGDAAHAIVPFFGQGMNCGFEDCRVLDELIDKHKQD
                     WGKILKEYQTLRKPDGDAIADLAINNFTEMRDKTADPKFLLQKKIEARLHEKYPDKWI
                     PAYSQVTFSPHIRYSEALKRGQMQEAIMQDVMAMPGIEEKWENVEVEDVILRRLNS"
     gene            complement(3760..>4064)
                     /locus_tag="PK977_16175"
     CDS             complement(3760..>4064)
                     /locus_tag="PK977_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRF19713.1"
                     /translation="RLHKKRGLLSAYLSFVINDCNRKASEKIIEIITPENEKERGCQV
                     SMLMLKNGKSIFDELTRHGVIADWREPNVIRVAPVPLYNSFEDIWTFGDVVASILK"
BASE COUNT         1143 a          918 c          774 g         1219 t
ORIGIN      
        1 actgctacct gtgcaaaagg ggcgttgctg atccacattt tggcgccgtt caatatcaca
       61 tggtcaccgg catctttaaa attggtcagc atactggcag ggtcacttcc atgattgggc
      121 tccgtcaaac cgaaacatcc cagccattct ccactggcca gcttaggcaa atattttttc
      181 ttttgttctt cgcttccata agcgttgatc gggaacataa ccaaggagcc ttgaaccgat
      241 gcagtagacc ggacaccgct atctcccctc tctaattcct gcatcatcag gccatagctg
      301 atataatcca gtcctccacc accgtattct acaggaacag ttgggccaaa acaacccaat
      361 tcacctaatt gctttactat atgctgggga aattctgcct tctgtgcata atcctcaatg
      421 atcggggaaa tctctttttt aacataactg cgaacagtgt cacgaatcat cttatgctct
      481 tcggtaagca gttcatccac taagaaataa tccgggcttt gaaaaaggtc tgtacgggct
      541 gccatcgtta ttgaatattt aagattgtag atttataatt aactggtgca aaggtaagaa
      601 aacggtagca gcggctattc tttgtgaaac gagtgtttta tttttatccc tgtaaaaaac
      661 aaccaaatga aatatgcgct tatttccctt gttttccttt tttgctgctc agtagtaacc
      721 tatgcccaaa aagactcaac aaaatccggg gctgaaccaa gggttaagca attctggatg
      781 gtaattctta aaactggacc caaagacaaa gaaattaccg attccattca acggaaaacg
      841 ctttttgccg gacatttttn nnnnnnnnna gattacacaa agaaggtatt ctaaaagcag
      901 caggtccttt tggtaaaaat gattttacct ggagagggct ttttatcatg gattgtgcaa
      961 caaaagagga agccgaaaac catgtaaaaa ccgatccgac tgtggctgcc ggtgttttta
     1021 tttatgatat agttccctgg tggagtgaac cgagcggtag ttttaaaacg ggcaaaccgg
     1081 agaaaaagga agaatgagct acccggcaaa agaaataata cgaaattttt tgtctgtagt
     1141 tgccggcgta atgattgctt taataataat tctacccatt ggattatttg caggattgtt
     1201 ggttttttca gattcgaata ttccaaaatc gaaagaaact ctctctaaac ttttattgat
     1261 ttttggatta atagccggat gtttacttgg aggctatacc accgtaaaga tgtcaacaag
     1321 aaaagacctg gttcatgcat tcattaccgg tattgtgctg acttttctct atgcattggt
     1381 taatgacttt gaatttgact ggcggtaccc ggacacaggg atagcttacg caggattcat
     1441 acccattgtg atgattggag gtataatcgg agagagaaaa aaacgaacta cttcaaatag
     1501 ccactcatct cctcctggta ctccttagcc acttcccttg ccttctctgc aaaatcctgt
     1561 cctgcagatg cgtaaagtat agcacggctt atgttcacta aaatgccgca atctttgatc
     1621 attgcttctt cagaaacgtc tttaagacta ccgccctgtg cgccgacacc aggcaccaga
     1681 taaaaatgat ggggtgtcag ctttcgtaca ttactcaact ctccttctct tgtggcacct
     1741 atcacaaaca taagattgtc ttcagtgccc catgcagaaa cggtttcaag cactttttca
     1801 tagaaaaatt ctccgccggt atgcagcagt tcaaaatcat tggctccttt attggaagtt
     1861 aatcccaata caattgccca tttacctttg tgctgtaaaa agggcttcac actgtcctct
     1921 cccatgtacg gtgccacagt aatggcatca aaaggcaacg tttcaaaaaa agctttagca
     1981 tattgatcag aagtgttgcc gatatcaccc cgtttggcat cagcaatttt gaaatgttca
     2041 tcgcctatat aacgaaccgt tttctccatt gcctcccagc ctttcactcc caaggcctca
     2101 taaaaggcag tgttgatctt atagctgacg cataaatcct ttgtagcgtc aatgattgct
     2161 ttattaaatg caaatacggg atccggatct gttgaaaggt ggcgtggaat ttttgttata
     2221 tctgtatcaa gtcctacgca gaggtaagat ttctttttac ggatttgctc aatgagttgc
     2281 agtcgggtca taaaggcaaa gctaaatgat taagtagttt ctgaaatact gaatggcatt
     2341 ttagtgtatt gcaaccctaa ttaacaaatt aactatttaa ccttcttaaa ataacatcct
     2401 ctacttctac attctcccac ttctcttcta tccccggcat cgccatcaca tcctgcatga
     2461 ttgcttcctg catttgtcct ctttttaatg cttcgctata gcggatatgc ggactgaaag
     2521 tcacctgcga ataggcaggt atccatttat caggatattt ttcatgcagt cttgcctcta
     2581 tctttttttg cagcaggaat ttggggtcag ccgttttatc ccgcatctcg gtgaagttat
     2641 taatggccaa atcagcaata gcatcaccgt cgggctttcg aagtgtttga tattctttca
     2701 atattttgcc ccagtcctgc ttatgcttat caatcagttc atccagcacc cggcaatctt
     2761 caaagccaca gttcatcccc tgaccgaaaa agggtacaat ggcatgggct gcatcgccga
     2821 ttaaagcaaa cttatcccct cttatccagg gaaagcattt cactgtaacc agggaagaag
     2881 tgggattggt aaaaaattca ttgatataat ccggcatcag ctttactgca tccggaaaag
     2941 ttctttcaaa gaagctttta actgcctcag gagttgacag tttttcaaaa gaagcctctc
     3001 cttcaaaagg gaagaacaac gtacaggtaa aggtcttgtc caaattgggc aaagcaatca
     3061 acatatagtc ttttctcggc catatgtgca gggcattgac ctgcatggca aactcaccat
     3121 ccgatgttgg aggaatggtc agttctttat acccacagtc aatatagtac tggctataat
     3181 taaatttacc atgctgcaac tggtgctgca aacgagcagc agaaaatgca ccgtcagcgc
     3241 cgaagatcag gtctgtttta aaagaaggaa gcggtttgtt tttattgctt tcaaactcaa
     3301 taacctcatt gctccagtcg atcttgctca gcttttcatt gaaatatatt tccactccct
     3361 gctcctctgc aaggtccatc agtttcatgt taagagtacc tcttgaaaca gaattgatat
     3421 actgcccttc tttaccatac ggctgaaaag cagaactgcc atccaggttg tgaatgtagc
     3481 ggccatgcat gggaatagaa atctttttga tttcttcagc cagtccaact ttttcaaggg
     3541 ccagcaatcc cctgtcgctc aaagcaagat ttatagaacg gccagccgac attttttctt
     3601 ttcgcatatc gccgcggcgt tcatatactt ttaccttata cccccttttg gataaataga
     3661 tggaaagcaa tgatccgacc aagccggcac cgatgatggt aatgttttct ttcataccct
     3721 aaacccctga aggggctttt cattttaaaa tttgaaaaat tattttaata tggaagcaac
     3781 aacatcgcca aatgtccata tatcttcaaa tgaattgtat aacggaaccg gagctactct
     3841 tatcacattc ggttctctcc agtcggcaat aactccatgc cgggttaact catcaaaaat
     3901 agatttgccg tttttgagca tcagcataga tacctggcaa cctcgttcct tctcattctc
     3961 cggtgttatg atttctatta ttttctcaga cgctttgcgg ttgcaatcat taataacgaa
     4021 tgataaataa gctgacagca atcctctttt cttgtgcaac cgct
//
LOCUS       DAORLS010000273         4053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12075_length_4053_cov_1.455478, whole genome shotgun sequence.
ACCESSION   DAORLS010000273 DAORLS010000000
VERSION     DAORLS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12075_length_4053_cov_1.455478"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>238
                     /locus_tag="PK977_16180"
     CDS             <1..>238
                     /locus_tag="PK977_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19714.1"
                     /translation="TSNYNHYANAIALSGNDVYITGFENRPTEWKCQVWKNGQHQYSL
                     SDTYSEGRAIAVSGSDVYVGCYLYEAPSTRMAAL"
     assembly_gap    239..248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <249..833
                     /locus_tag="PK977_16185"
     CDS             <249..833
                     /locus_tag="PK977_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19715.1"
                     /translation="QLPPTIFDTSINSYSPAVNDLAISGADVYAGGFADNMGKIWKNG
                     TDMGLSNTAGLTIKAIAVAGTDVYATANVGTNTIRYWKNNTYTDITVTGGRQIYVSDI
                     AVEGTDVYVCGWENNGTIGIAKYWKNGSEFSLGDGIRSSTAWGIAVKNGKVYVAGNSA
                     AGSVSFIDYATIWENGVARVIGARNSRATSIAVK"
     gene            1075..1569
                     /locus_tag="PK977_16190"
     CDS             1075..1569
                     /locus_tag="PK977_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19716.1"
                     /translation="MQILTNDSLSFSLVNDFGNILGSIRYLDDELKEATLHTEHLYQI
                     TNNKPGVWTFIHQDSKEISSKMKVSTGGKIIVESKRLRFWFFKTLNWNLRFVLLNKER
                     EELLALKPAINWKFKTHDFILQLNEELTTECSSFLILQALHCARCSLGMMYDKTVPPI
                     VSVC"
     gene            1572..>1846
                     /locus_tag="PK977_16195"
     CDS             1572..>1846
                     /locus_tag="PK977_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19717.1"
                     /translation="MDNRKLYIIKTLLLTVVFFTKTSISQNCLCDVWKKEEKINTDLL
                     INNPEPACKIKGYEQKAQVYLDTKELDSAQQSLEMAESLLKKQGCKE"
     assembly_gap    1847..1940
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1955..>3227
                     /locus_tag="PK977_16200"
     CDS             1955..>3227
                     /locus_tag="PK977_16200"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF19718.1"
                     /translation="MLTVVQASNNSEQEADVMLSIAQIFSRMGQADRSLDYLHKALLI
                     IEKIKSSTVKTDLLNKAGSRYYFLFQDTKEKLLLDSATLFFNQAITIARQTGYKKGLQ
                     VSYNKMNTISYQQKDYKQALLYIDSSIALANPGELNNAVGTSYGDKGNILLKMGNFPE
                     AKKWADSCLNYFKKLKFPPLIANAYSLVAEIADSLGDYKTAYRALYAEKKITDSLNTQ
                     ENAKAVNEVEKKYNQAKNEKTIKELSQQKLIYALLGLGGLLVLGLVAFYFRQQALKNK
                     QKILETEQRLNRARMNPHFFFNALTAMQRFAMKENDGKALASNLSKFSHIMRETLEST
                     YKEYVTIKQEIEFLKEYLEIQKMRFPQIFTYSLMVDGEIEPGDVMIPSMIIQPFIENS
                     IEHGFAGINYEGEIRVDFKQAHKSVEIVIHDN"
     assembly_gap    3228..3256
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3416..>4053
                     /locus_tag="PK977_16205"
     CDS             3416..>4053
                     /locus_tag="PK977_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF19719.1"
                     /translation="MRILIIDNEKIVRELLRDMIEAIEPGKNSIGEANGVATGLQKIK
                     SFSPDVVFLDVEMDDGTGFDLMQKIQQPTFQLIFTTAHNQYAIQAFKCSAIDYLLKPV
                     DFTELGNALQKAATHISGNTLNKQLEVLVQQLSSKNNDKQIVLKDSEASYFVKVSDIL
                     YCEAEGSYTKFYLAADSPIVISKNLSTYEDLLNAFGFIRTHHSYLVNPSKIK"
BASE COUNT         1356 a          739 c          776 g         1049 t
ORIGIN      
        1 ttacgagcaa ttacaaccac tatgcaaatg ctattgcgct aagtggcaat gatgtttaca
       61 taacggggtt tgaaaacaga cctaccgaat ggaagtgcca ggtttggaaa aacggccagc
      121 accagtatag tctgagcgac acctacagcg aagggagggc aatagctgtt agcggttcag
      181 atgtatatgt gggttgctac ctttacgagg ctccatcgac aagaatggca gctttatgnn
      241 nnnnnnnnca attgccacca accatatttg acacttccat aaacagttat agccctgcgg
      301 taaatgacct tgccatttca ggtgcagatg tttatgcagg aggatttgca gataacatgg
      361 gtaaaatttg gaaaaatggt actgatatgg gtctgtcaaa tactgccgga cttaccatta
      421 aagctatagc tgttgcaggt actgatgtat atgcaacagc taatgttggt accaatacta
      481 taaggtactg gaaaaacaat acttatactg atattactgt aaccggcggc aggcaaattt
      541 atgtttctga tattgccgta gaaggaaccg atgtatatgt atgtggctgg gagaataatg
      601 gcactattgg aatagccaaa tactggaaaa atggttcaga atttagctta ggggatggaa
      661 taagaagttc aactgcctgg ggaattgctg taaaaaacgg aaaggtatat gttgccggca
      721 actcagccgc cggaagtgtt agttttatag attatgccac catttgggaa aatggtgtcg
      781 caagagttat tggcgcaaga aatagccgtg ctaccagcat tgctgtaaaa tagaatacca
      841 aaaattatca gccggcagat gtttacctaa aattaaatgt ctggcggctt tcttattgta
      901 tgaaacaaag tactataact tccaggtcaa ttttgtaaat aaagtttcgc ttaacagacc
      961 tttaacgact gaataataaa tggccacact tatccacaaa taacttacag ttataaaaac
     1021 tgactattcc ccctgttagt agtgtataat ttggtactaa ccggaagaaa gaccatgcaa
     1081 attttaacca atgactctct atcctttagc cttgtcaacg actttggcaa tattcttggt
     1141 tccatccgct acctggatga tgaactgaaa gaagcaacac tccacacaga acatttatat
     1201 caaataacga ataataaacc aggtgtttgg acatttatcc atcaagattc caaagaaatt
     1261 tccagcaaaa tgaaagtaag tacaggcggg aagataatag tggaaagtaa gcggctacga
     1321 ttttggtttt ttaaaacatt gaactggaat cttcgttttg tattattaaa caaggaaaga
     1381 gaagagttgc tggctctaaa acctgcgata aactggaaat ttaaaaccca tgatttcatc
     1441 ctgcaactga atgaagaact tactacagaa tgcagtagtt ttcttatttt acaggcactg
     1501 cattgtgcca ggtgcagcct gggtatgatg tatgacaaaa ctgttccgcc aatagtaagc
     1561 gtatgctaat catggataac cgaaagctat atatcatcaa aactttactc cttacagttg
     1621 tttttttcac aaaaaccagc atttcacaaa actgcctttg cgatgtttgg aaaaaagagg
     1681 aaaaaataaa taccgacctg cttatcaata atcctgaacc tgcctgcaaa ataaaagggt
     1741 atgaacaaaa ggcacaagtt taccttgata caaaagagct tgattcagcc caacaatcgc
     1801 tggagatggc cgaatcttta cttaaaaaac aaggatgtaa ggaaaannnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn actacagttt aaaaatgctg actgttgtgc aagcttctaa
     1981 taatagtgaa caagaagcgg atgtaatgct cagcatcgca cagatctttt cccggatggg
     2041 acaagcagac agaagccttg actatctgca taaagcactt ctaataatag aaaagataaa
     2101 aagttcaacc gttaaaaccg atctattaaa caaagcagga tcccggtact actttctttt
     2161 tcaggatacg aaagagaaac tattgctgga ctctgctacc ttattcttta accaggccat
     2221 tactattgcc cggcaaacag gttataaaaa aggattacag gtaagctata ataaaatgaa
     2281 taccatttct taccagcaaa aggattataa acaagcttta ttatatattg attcatctat
     2341 tgcattggca aatcccgggg aactgaataa tgccgtggga accagctatg gtgacaaggg
     2401 taatattttg ctgaaaatgg gaaatttccc ggaggctaaa aaatgggctg actcctgttt
     2461 aaattacttt aaaaaattaa aatttccccc actaatagcc aatgcataca gcctggtagc
     2521 agaaatagct gacagtcttg gtgattataa aacggcttac cgggccttat acgccgaaaa
     2581 gaagattaca gacagcctga acacccagga aaatgccaaa gctgtaaatg aagtggaaaa
     2641 aaaatacaac caggctaaaa atgagaaaac gattaaagaa ctctcccagc aaaaactaat
     2701 ctatgctctt cttggattag gcggtttgct tgtgctgggg ttagtagcgt tttatttccg
     2761 gcaacaggcg ttaaaaaaca aacaaaagat tttagaaacc gagcagcgct taaaccgtgc
     2821 ccggatgaac cctcattttt tctttaatgc acttacggct atgcagcggt ttgccatgaa
     2881 ggaaaacgac ggaaaagccc ttgcttccaa tctttctaaa ttctcccata ttatgcggga
     2941 aactttagaa agtacttata aagagtatgt aacaataaaa caggaaattg aatttttgaa
     3001 agagtatctt gaaattcaaa agatgcgttt cccgcaaata tttacttatt cacttatggt
     3061 tgacggcgaa attgaacccg gcgatgttat gattccctct atgattattc agccttttat
     3121 tgaaaacagt attgagcatg gatttgcagg aataaattat gagggagaaa tccgggtcga
     3181 cttcaaacag gcacataaat ctgtggaaat agtcatccac gacaatgnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnngtaa aaacaacgag catatcagcc gggccagcca gataataaaa
     3301 gacaggatat atttgctgaa tattaaactg aaaacaaaag ccgatttttc aatagataac
     3361 aatagcaacg gtaaaggtgt agtggtaaaa attcatctcc cattgatata caggaatgag
     3421 aatactgata attgataacg aaaaaatagt ccgggagctc ctccgggaca tgattgaagc
     3481 catagagcca ggcaaaaatt ctattggaga ggcaaatggt gtagcaacag gtttgcaaaa
     3541 gattaaaagc ttcagccccg atgttgtatt tcttgatgta gaaatggatg atggcaccgg
     3601 cttcgacctg atgcaaaaga ttcaacagcc cacctttcaa cttatattca caacagcaca
     3661 taaccaatat gccatccagg catttaagtg cagcgctatt gattacctcc ttaagccggt
     3721 tgattttaca gaactgggaa atgccctgca aaaagctgcc acccatatct cgggcaatac
     3781 acttaataaa caattagaag tactcgtcca gcaactcagt tcaaaaaaca atgacaagca
     3841 aattgtacta aaagacagcg aagccagtta ttttgtgaaa gtttctgata tattgtattg
     3901 cgaagcagaa ggctcttata ccaaatttta tcttgctgcc gatagcccga ttgttatttc
     3961 aaaaaacctg agtacttatg aagaccttct gaatgcattt ggttttatca gaacacatca
     4021 ctcttacctt gtaaacccat ccaaaattaa aat
//
LOCUS       DAORLS010000274         4047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12101_length_4047_cov_1.645541, whole genome shotgun sequence.
ACCESSION   DAORLS010000274 DAORLS010000000
VERSION     DAORLS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12101_length_4047_cov_1.645541"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>254)
                     /locus_tag="PK977_16210"
     CDS             complement(<1..>254)
                     /locus_tag="PK977_16210"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRF19720.1"
                     /translation="NPVNANIVLLKKSVEQIYRRSSKSFVYLYDAVAGKLTKLENEKV
                     LHPTFSPDGSKIAYVKNNNLVMYDLATKSARAITTDGKWN"
     assembly_gap    255..380
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(742..2001)
                     /locus_tag="PK977_16215"
     CDS             complement(742..2001)
                     /locus_tag="PK977_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF19721.1"
                     /translation="MADIFDRLLKHYGPIGQHRERAHGYFAFPKLEGDIGSRMKFRGK
                     EVIVWSLNNYLGLANHPEVRKADAEGAKNYGLAYPMGARMMSGNSNLHEQLESELAAF
                     VHKEDAALLNYGYQGMVSIIDVLCSRHDVIVYDAESHACIIDGVRLHPGHRYVFKHND
                     LEDCEKQLQRATALIEKQKAGGILVITEGVFGMAGDQGKLKEIAALKDKYQFRLLVDD
                     AHGFGTLGKTGAGAGEEQGCQDQIDLYFSTFAKSMASIGAFIAGDRKIIDYIRYNIRS
                     QIFAKSLPMPLVVGNLKRLELLRNHPEYKEKLWSNARKLQEGLKARGFDIGNTDSVVT
                     PVYMKGDVPEATAMVMDLRENYGVFASIVVYPVIPKGHIIYRLIPSAVHTDEDIEQTL
                     KAFSETKKKLDAGEYRVEAIPDMAENK"
     gene            2252..2518
                     /locus_tag="PK977_16220"
     CDS             2252..2518
                     /locus_tag="PK977_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19722.1"
                     /translation="MAETGFTRRDISFTLAAAIFTMLTGGSYKSGGGFYIGGGEFNSG
                     GDRVYKGGDEFYIGGCRIYIDGGEGKLPGKTGLHYLNPDWGRFD"
     gene            complement(2583..>2939)
                     /locus_tag="PK977_16225"
     CDS             complement(2583..>2939)
                     /locus_tag="PK977_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19723.1"
                     /translation="VMYVVFKSSQDAVILMMISAATVWLLVYLVAHINLIVLRKRYPR
                     YTRPYRSPFYPLPQILGIISLLYLIVNNSPTPEMTKDVYLNVFIIVGLTALYAVFWIK
                     FKMKKGLFKAEPIDNV"
     assembly_gap    2940..2957
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2958..>3209)
                     /locus_tag="PK977_16230"
     CDS             complement(<2958..>3209)
                     /locus_tag="PK977_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF19724.1"
                     /translation="KVPGTELVNNSIPHWILIQSVLGENTKLLMAILAVTAAGSSFST
                     GMAAISRMLYGMAKNNQLPRIFGAVHPKFKTPWFGLLFPC"
     assembly_gap    3210..3319
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3320..3976)
                     /locus_tag="PK977_16235"
     CDS             complement(<3320..3976)
                     /locus_tag="PK977_16235"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF19725.1"
                     /translation="MNDLKIVNTPLKRTLGLFSLIAVGVGMVIGQGALVTILQGVGIN
                     ASAFFIAMLFAFALALTYIFTFTELSLMMPKAGGISSYTEVAIGHLPAIVITIGGYLG
                     LAIFAGAADIFLLNYVFDVLYPGTFSNIGLWIYVIIIIFNFFGVDVFASVQNILAFTM
                     LALLLLIGCVGVSSSEASDIPYSNLVSGFGSLHGNVLSLTVLALWAFMGMEFVCPMVE
                     E"
BASE COUNT         1054 a          827 c          885 g         1027 t
ORIGIN      
        1 gttccatttg ccgtcggtgg tgatggctct tgcacttttg gtggcgagat catacatcac
       61 caggttattg ttctttacat aagcgatctt gctgccatcg ggtgaaaaag tggggtggag
      121 tactttctca ttttccagct tcgtgagttt accggctact gcatcatata aataaacaaa
      181 tgatttggag gaacgtcggt atatctgttc taccgatttt ttcagcaaaa cgatattggc
      241 atttaccggg ttgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn taaatgtctt ctaatgtgat aggttttttt tgcgctacca
      421 gtgctaaggg caggataaac aagaacagga aaagcttacg cataatcaga gaattttttg
      481 agggtataaa agtacagcaa acagagcaaa gggggctgcc gtacaaataa aaggggcttt
      541 tttcccagaa agaagatttc aagcaaagac gctgagattt cccacaaagg cacaatgaac
      601 acaaagataa tttcctgcaa tggctttaag tcgcaagagg ttgcctatgt gaaaaagggc
      661 acaaagaagg acggcagctt taacgctgcc gtcaggaaac aatttatctc acccttcgac
      721 aagctcaggg tgacagtgtc gctatttatt ttctgccata tccgggatgg cttctacacg
      781 gtattcaccg gcgtccagct ttttcttggt ttctgaaaaa gcttttaatg tctgctcaat
      841 atcctcatca gtatgaacag cggaaggaat gaggcggtag atgatatgcc cttttggaat
      901 tacgggatat actacgatgg aagcgaaaac accataattc tcccgcaggt ccattaccat
      961 ggcggtggct tcgggtacat cacctttcat ataaacaggt gtaaccacag aatctgtgtt
     1021 gccaatatca aaacctcttg cttttaatcc ttcctgcagt ttgcgggcat tgctccagag
     1081 tttttcttta tactccgggt ggttgcgcag caattcgaga cgcttcaggt ttcctactac
     1141 caatggcata ggcaggctct tggcgaagat ctgtgaacgg atattgtagc ggatataatc
     1201 tatgatcttt ctgtcgccgg caatgaaagc gccgatagac gccattgatt tggcgaacgt
     1261 agaaaagtaa agatctatct gatcctggca gccctgctct tcaccggctc cggcacctgt
     1321 tttacccagg gtaccaaatc catgggcatc atccaccagc aggcggaact ggtatttatc
     1381 tttcagcgct gcgatctctt tcaatttacc ctggtcgccg gccatgccaa atacaccctc
     1441 ggtgatgaca agtatgccgc cggctttttg tttttcaatc agcgcagtag ctctttgtaa
     1501 ttgcttttcg caatcttcca ggtcgttatg cttgaataca tagcggtgac cgggatgcag
     1561 ccttactcca tcgatgatgc aggcatggct ttcggcatcg taaacgatca catcatgacg
     1621 gctgcacagc acatcgataa tgctgaccat gccctggtaa ccgtagttga gcagggcggc
     1681 gtcttcttta tgtacaaagg cagcgagttc gctttcgagt tgttcgtgca ggttactgtt
     1741 accactcatc atgcgggcac ccatggggta ggccaggccg tagttctttg cgccttcggc
     1801 gtcggcttta cgtacttccg gatggttggc caggccgagg tagttattaa ggctccatac
     1861 gatcacttcc ttgccacgga atttcatgcg gctgccgata tcaccttcca gtttgggaaa
     1921 tgcaaaatat ccatgtgccc tttcacggtg ctggccgata gggccgtaat gcttcagtaa
     1981 acgatcaaaa atatctgcca tacgatttga atttcggatt tacgatttca ggttgcagat
     2041 ttggctgaaa gacaggtaca tgagctgcaa ccctgtaaga tttagcggtg caaaaataag
     2101 gttttgaggg ttttggcggc gggtgaagtt tatcaggggt gatgatggcc tttcaggatt
     2161 gatacatctt ccgggtcaaa agttgggatg aaggagtgtg tatttacatt gtcgaagacc
     2221 agttttacag ggcggagcaa tgtgttttac aatggcggag acgggtttta caaggaggga
     2281 tatctctttt actttggcgg cagccatttt tacaatgctg acaggcggct cttacaagag
     2341 cggaggcggg ttttacattg gcggaggtga atttaacagt ggcggagata gagtttacaa
     2401 gggcggagat gaattttaca ttggcggatg ccgcatttac attgacggag gggagggtaa
     2461 gctaccggga aagacaggtt tgcattatct gaacccggat tggggaagat ttgattgatt
     2521 ttttagacaa ttcttgttga cggcagtgta acctatgaat taaattattt caattcttat
     2581 tattaaacat tatcaatcgg ctctgcctta aataagcctt tcttcatttt aaactttatc
     2641 caaaaaactg catagagcgc cgtcagtccc acgattataa atacgttgag gtaaacatct
     2701 tttgtcattt caggagttgg agagttattg actattaagt atagcaaact gatgattcct
     2761 aaaatttgcg gcagcggata aaacggagac cggtaaggcc gggtatatcg gggatatctt
     2821 tttcgcagta cgatcaggtt gatatgtgcc accaggtaca ccagcagcca cacagtagct
     2881 gctgaaatca tcatgagtat aacggcatcc tgtgaacttt taaaaaccac atacattacn
     2941 nnnnnnnnnn nnnnnnnaca gggaaaaagc aggccaaacc agggtgtttt gaatttcgga
     3001 tgaacggcac caaaaattcg gggtagctgg ttgttttttg ccataccata taacattcgg
     3061 gatattgcgg ccataccggt actgaaagag ctaccggctg cagtaacagc cagtatggcc
     3121 atcaatagct ttgtattttc ccctaaaact gattgaatca atatccaatg cggaatggag
     3181 ttattaacca gttccgtacc gggcaccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnt tcttccacca ttggacatac gaattccatc cccataaaag
     3361 cccacagtgc taaaacggtt aacgaaagaa catttccatg cagggaaccg aaaccggata
     3421 ctaagttact atagggtatg tcactggctt cagaactgct caccccaaca catccaatga
     3481 gcaacaacaa agccagcatg gtaaatgcaa gtatattttg tactgacgca aacacatcaa
     3541 caccgaagaa attgaaaata ataatgataa cataaatcca tagaccgatg ttgctaaagg
     3601 tgcccgggta gagtacatca aatacataat ttaagaggaa aatgtctgcc gcgccggcaa
     3661 aaatggccag gcccaaataa cccccgatgg ttattacaat ggcagggagg tgtccaatcg
     3721 ccacttcggt atagctgctt ataccaccag ccttcggcat catcagcgac aactccgtaa
     3781 atgtaaaaat gtaggtaagc gccaacgcaa aagcaaaaag catagctata aagaaggctg
     3841 aggcatttat ccctacaccc tgcagtatag ttaccaatgc accctggcca attaccatgc
     3901 ccacaccaac ggcaatcaga gaaaacaagc ccagtgttcg tttgagcggg gtatttacaa
     3961 ttttcaaatc attcataaac taagtcatca tcatacatcg ccgctaacgg ccagagcttg
     4021 ctgctgtact ggtattttat aatgtcc
//
LOCUS       DAORLS010000275         4009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12244_length_4009_cov_2.508346, whole genome shotgun sequence.
ACCESSION   DAORLS010000275 DAORLS010000000
VERSION     DAORLS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12244_length_4009_cov_2.508346"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..724)
                     /locus_tag="PK977_16240"
     CDS             complement(107..724)
                     /locus_tag="PK977_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF19726.1"
                     /translation="MVKNKKDTTTEEVILAAAKKVFVQKGMAGARMQDIADEAGINKA
                     LLHYYFRNKEKLFEVIFMEAAEKLFPKINAIFEADQPLFEKIENFCEEYISIVMDNPY
                     LPLFVLNEINQDPDYFLKKVWTGKSKPNPVKLLEQIDKEVRKGTIKRINPMHLLMNLI
                     SMTIFPFVAKPMFQKNLGMSEVQFKMAMEERKKEIPRFIIDSLKK"
     gene            complement(1222..1932)
                     /locus_tag="PK977_16245"
     CDS             complement(1222..1932)
                     /locus_tag="PK977_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19727.1"
                     /translation="MRFILPIMCWLWPLFAFPQAKDSLQKLQSKPLPSKPAANIAKDY
                     KPEIFTSGFIDIVNNGQVNASARFIRLYIGEPGKFAIPISFYGGVSNNTFQNNTPGTI
                     FSKSNSHLVNQYINPLSGLINISVEGVQFFSKKEKITRSGLLYQIGERVLSGSRIGEI
                     SNPLTGKPINFLNSFAATGLYFQTGAWERNNTKNVGVFWLALRYHACKSNAKQVKEFL
                     PSVETNGFYHGWSGGFGI"
     gene            complement(2033..2875)
                     /locus_tag="PK977_16250"
     CDS             complement(2033..2875)
                     /locus_tag="PK977_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19728.1"
                     /translation="MTIQLTSGKQGGVIRIILVCLFSQFTLQAFTQDTNKNLREYLFQ
                     KSSFKLQIGYGVIPKGVIEVKEGPYDLYTKPHSIYSVGLIYQVILNPSWSVNTGISFM
                     IIKSNFFEKIPINELIGTGISRREDSPPIIYYKGAYPRFSLPVLLQKCTKIINKSFFE
                     FNCGFNISYSGFSKDETIIMNVADNNNQSINIFNANYKSNNNHKPWVGLLLGCGKGFL
                     LKKLNVLTVSLSAELNTTKFLSADYEITIPNKPISTGNYSVKGPLLQLSLGYTFREVH
                     KNKD"
     gene            complement(2885..>4009)
                     /locus_tag="PK977_16255"
     CDS             complement(2885..>4009)
                     /locus_tag="PK977_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19729.1"
                     /translation="GGGANTNNPFSLTITAGNLSAETAANFYQTDPTATAEATLGTIA
                     TVYAGVTITYEAEGCILKFNSLGDVNTVLNQLEADDDNYNDDYDSQYPNASEDELDDL
                     DEQNNFDNMKVYRDFEALFGGFCSKRAELEAIENTWLSNNFNGTDPDDVDLTFDDAEN
                     TIFNSSYSFKIGTDVYQLTSTGMYKNGILQDEGGNSMNRKPDNFIELPEYGVLSGPAS
                     FPNNYWSFTDISPANVLSNDFFTVCKSNKKNKIFQEYDNGSKRVKATVSITSTVVRNG
                     VHGKIKNYKSKNGNWKRSRAQLAVYCFGTYYTNLCVDAHQFTDRKPLSGTKKRAKIST
                     RRHSPGHIPVQGTVWKTFTGEVAMYGEVGNAVQGQLTLTF"
BASE COUNT         1140 a          809 c          708 g         1352 t
ORIGIN      
        1 caggggctgt gccagcagcc aacccgggat taataatatt atcagcaatc taatcttctt
       61 catgatcgac tattgaatga gccggcattt gtctgccgga aaatggttat ttctttaatg
      121 agtcaataat aaaccgggga atttcctttt tcctctcctc catcgccatt ttaaattgca
      181 cttcactcat gcccaggttt ttttgaaaca ttggctttgc tacaaaggga aatatcgtca
      241 tagagatcag gttcatcagc aggtgcatgg gattgattct tttaatagta cctttcctga
      301 cttccttatc aatctgctcc aaaagtttta cggggttcgg tttagatttt cctgtccaaa
      361 cctttttcaa aaaataatca gggtcctggt ttatttcatt taacacaaat aatggcaggt
      421 agggattatc cattactatt gagatatact cctcacagaa gttctctatc ttttcaaaca
      481 gcggctgatc tgcttcaaaa atggcattga tctttggaaa aagcttttct gccgcttcca
      541 taaagatcac ctcaaacagc ttttctttat tacggaaata ataatgcagc aatgctttgt
      601 tgataccggc ttcatcggca atatcctgca tccgggcgcc tgccattcct ttttgtacaa
      661 atactttttt ggcggcggcc agtataactt cttcagtagt cgtatctttt ttatttttaa
      721 ccatatgatt taaccaaatg gttaacaaaa ataaactctt atttttaaca aacaaattat
      781 ttttcaaatt caccaaccct tgtatggaat acgcttcacc agcagcaccc tgcaatcccc
      841 tctcctatag gagaggggcg aacgaaacat cggggtgtaa caaatcgtca aacggggtga
      901 ggttgctgcc tacttcatcg aatattgaaa gggaaactgg taaatgtgca ggttgtattt
      961 tatagtcgga gtattgcttt tgcttcttcc tcacccccaa aacgccaact gctcaccagc
     1021 agcaccctgc aatcccctct cctttaggag aggggcgaac gaaacgtttc attttgtgca
     1081 aaccgctata tggggtgagg tacttattgc ttacttcatc gaatactgaa acgaaaactg
     1141 gtaaataggc aatccgtatt ctatctccgg tgctttgctg tatttgtaat aaatagcctt
     1201 gatgttgaca aattaattga tctaaatccc aaacccgccg gaccagccat gatagaaacc
     1261 gttggtttca acagatggca agaactcttt aacctgtttt gcattgcttt tgcaggcatg
     1321 atagcgaaga gccagccaaa agacaccaac atttttggta ttgttgcgtt cccatgcccc
     1381 ggtttgaaaa tacaaaccgg ttgcggcaaa gctgttgaga aaatttatgg gcttaccggt
     1441 aagcggatta cttatctcgc caattcggct accgcttagc accctctctc caatttgata
     1501 taataaacct gaccgggtta ttttttcctt ttttgaaaag aattgtactc cttctacgct
     1561 gatgttgata agaccgctga gaggattaat gtactggttt accaggtggc tgttactttt
     1621 ggaaaatata gtaccgggtg tattgttttg aaaagtatta ttacttacgc caccataaaa
     1681 agaaatggga atggcaaatt tacccggttc accaatgtac aatcttataa accgtgctga
     1741 agcatttacc tggccgttgt ttacgatgtc aataaagccg gaggtgaaga tttcgggttt
     1801 gtaatctttt gcaatattag cagcaggctt agatggcagc ggtttgcttt gcagtttttg
     1861 caaactgtct tttgcctggg gaaaggcaaa caacggccat agccagcaca ttattggcaa
     1921 aatgaatctc actttcctgt tcccttttat atttttttga actattcagc taagctaatt
     1981 ttatgaagcg atcaacttct ttacaatgca ggaatgtcaa ttttcaaata tctcaatctt
     2041 tattcttatg tacctctctg aatgtatatc caagagatag ttgcaaaagt ggacctttga
     2101 ccgaatagtt gccagtacta attggcttat taggtatagt aatttcataa tcggctgata
     2161 aaaactttgt agtgtttaac tctgctgaca aggaaactgt taatacgttt aattttttta
     2221 gtaaaaaacc tttgccgcaa ccaagtaaca aaccaaccca aggcttatga ttgttgttac
     2281 ttttatagtt agcattaaaa atatttatag attgattatt attgtctgca acattcataa
     2341 ttattgtttc atcttttgaa aatccgctgt aactaatatt gaaaccacag ttgaattcaa
     2401 agaagctttt attaataatt tttgtgcact tttgtagtaa taccggtaga ctaaatctcg
     2461 gataagctcc tttataataa atgattggcg gcgagtcctc ccttcttgat attcctgtgc
     2521 caattagctc attaatcggg attttctcaa aaaaattact tttaataatc ataaagctaa
     2581 tacccgtgtt tacagaccag gatggattta ggattacttg ataaattaag ccaactgagt
     2641 aaatgctgtg tggctttgta taaagatcat agggcccttc tttcacttca attactcctt
     2701 ttggaattac cccatagcca atttgcaatt tgaaactact tttctgaaat aaatattccc
     2761 ttaaattctt attcgtatcc tgggtaaatg cttgcaatgt aaattgtgaa aagaggcaaa
     2821 caagaattat gcgtataact cctccttgtt tgcctgaagt taattgaatt gtcattaaac
     2881 cattttaaaa agttaatgtt aactgaccct ggacggcatt tccaacttct ccatacatag
     2941 caacttcacc cgtaaaagtt ttccaaaccg taccttgtac tggtatgtga ccgggagagt
     3001 gtcttctggt ggatatcttc gctcttttct tagttcctga caatggcttt ctatcagtaa
     3061 attgatgagc atctacgcaa agatttgtat aataagttcc aaaacagtat actgccaatt
     3121 gtgcccgact tcttttccaa ttcccgtttt tgcttttata gtttttgatt tttccatgaa
     3181 ctccatttcg cactactgtc gatgttattg aaactgtagc ttttactcgt ttgctaccat
     3241 tatcatactc ttgaaatatc ttattttttt tgtttgattt acagaccgta aaaaaatcat
     3301 ttgatagcac atttgctggt gaaatgtctg taaatgacca ataattgttg gggaaggatg
     3361 cagggcctga caatacacca tattctggta gctcaatgaa attatccggt ttgcggttca
     3421 tagagtttcc gccttcatcc tgtaaaatgc catttttata cattcctgtg ctcgttaatt
     3481 ggtatacatc tgttccaatt ttaaatgaat agctgctatt aaaaattgtg ttttctgcat
     3541 catcaaaagt aagatcaaca tcatcaggat ccgtgccatt gaaattattt gaaagccagg
     3601 tattttctat cgcttccaat tcagctcttt ttgaacaaaa tccaccgaac agtgcttcaa
     3661 aatctctgta tactttcatg ttatcgaagt tattttgctc atccaaatcg tccagttcat
     3721 cttctgaagc atttgggtat tggctgtcat aatcatcatt ataattatca tcatctgcct
     3781 ccaactggtt taatacggta ttgacatctc caaggctatt aaacttcaaa atacaacctt
     3841 cagcctcata agtaattgtc actcctgcat aaacagttgc aatagtaccc aatgtggctt
     3901 ctgcagttgc tgtggggtca gtctgataaa aattagctgc tgtttctgct gacaggttac
     3961 cggctgttat agtcaatgaa aaggggttat tagtatttgc accaccccc
//
LOCUS       DAORLS010000276         3994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12310_length_3994_cov_1.872556, whole genome shotgun sequence.
ACCESSION   DAORLS010000276 DAORLS010000000
VERSION     DAORLS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12310_length_3994_cov_1.872556"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>432
                     /locus_tag="PK977_16260"
     CDS             <1..>432
                     /locus_tag="PK977_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19730.1"
                     /translation="PALMHGNSYTEASGMVGYNHSNSITKQENENRLLLDRLDHYEKN
                     KLRQPVVEKVLNQLINLINAILADPELGRPDEIRVELARDLKQSKDERNETYLRNIKT
                     DKEHKDIIERLNKEFPGLPVSRKMVEKYKLFIQQDGVCMYSG"
     assembly_gap    433..467
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            600..>1475
                     /locus_tag="PK977_16265"
     CDS             600..>1475
                     /locus_tag="PK977_16265"
                     /inference="COORDINATES: protein motif:HMM:NF037268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19731.1"
                     /translation="MKSKSDDDYKQFIERVSQLFASKKITKSKNDKLLMSGESIPDDF
                     INRQLNETRFISKESINLLKNICRNVLVSSGSVTEFLRREWGYNEVLKQLNWHKYEKA
                     GLISNDKIEGWSKRDDHRHHAIDALVVACTKQSFIQKLNTLNSSLTREEMYQRVKGNA
                     PEKWQGRKPLMEQYFQLEQPFTTQEVKEAVDNILISLKPGKKVATKSVNRKTGNQITL
                     TPRGQLHKEQVYGKIRKYSAKKSPLNAKFIHAELIADPTEKSLVINRLKQFDNDPKKA
                     FKDLEKNPIWIDSGKT"
     assembly_gap    1476..1511
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1512..>1865
                     /locus_tag="PK977_16270"
     CDS             <1512..>1865
                     /locus_tag="PK977_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19732.1"
                     /translation="LWEDHFVYKYTLDQNFKEKDIEFIVDIGIKEKVRQRFIEKKDTG
                     EHPLKNLEQEPIWLNKEKNIPIKSVRCFTGLTELVPLHTAENGRTLPASKKTENSKPV
                     DYVSTRNNHHIAIYQD"
     assembly_gap    1866..1998
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1999..2357
                     /locus_tag="PK977_16275"
     CDS             <1999..2357
                     /locus_tag="PK977_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19733.1"
                     /translation="IENQELLNNIPQNDWQFFLSMAQNELFVVSKTLEEIKNAIKENK
                     LNELSTQLYRIQKLSETDYYFRHHLETKLEKNSNEEKQSMFLGKVIRIRSLKALKELQ
                     LHKVQINSIGKIKLVN"
     gene            2360..3265
                     /gene="cas1"
                     /locus_tag="PK977_16280"
     CDS             2360..3265
                     /gene="cas1"
                     /locus_tag="PK977_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HRF19734.1"
                     /translation="MIKKVLYFGNPAYLKFRNEQLLIESDADDQESGQRSVPVEDIGL
                     VILDHSQITITQVTIAKLLENNAALITCDATHHPVGMMLNLDGNTLQSQKHQVQVDAS
                     QPLKKQLWQQTVTAKIRNQAALLKKEKAPSGYLESLAEKVKSGDSENAEAKAAAYYWK
                     RLFPDFLDFSRSRYGPPPNNLLNYGYAILRALVARALVSSGLLPTLGIFHRNQYNAYC
                     LADDIMEPYRPYVDQVVCQIVRMNGKFLEMTASMKKDLLSIPAMDVVLQGRKSPMMNA
                     VSRTTASLVACFEGESRKILYPELV"
     gene            3267..3611
                     /gene="cas2"
                     /locus_tag="PK977_16285"
     CDS             3267..3611
                     /gene="cas2"
                     /locus_tag="PK977_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRF19735.1"
                     /translation="MKSYYERINAYRIMWVLVMFDLPTETKADRKRYALFRKKILSDG
                     FQMFQFSMYIRHCSSKENAEVHQKRVKKMLPPKGHVGIMIITDKQFGMMEIFHGKDPA
                     PTPPTAQQLEFF"
BASE COUNT         1355 a          741 c          780 g          914 t
ORIGIN      
        1 tccggcattg atgcatggga atagttacac agaagcatca ggtatggtag gatacaatca
       61 cagtaatagt ataacaaaac aagaaaatga aaacaggcta ctgcttgaca gacttgatca
      121 ttatgaaaaa aataagctca ggcaacctgt agtagaaaaa gttcttaacc aactgatcaa
      181 ccttataaat gcaattttag ctgatcctga attaggtcga cctgatgaaa taagggttga
      241 attggcaagg gacttaaaac aaagcaaaga tgaacgaaat gaaacatact taagaaacat
      301 taaaactgat aaagagcata aggatattat agaaagacta aataaagaat tcccaggatt
      361 accagtatct aggaagatgg ttgaaaaata taaactattt attcaacagg atggagtttg
      421 tatgtactca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc aggggtgaag
      481 gaatagatgt ggaccacatc ataccccaaa ctcgtttatt cgacgactct ttccaaaaca
      541 aggtgctttc ataccaggaa gaaaacagaa aaaaagacaa tagtacagcc tatgattata
      601 tgaagtcgaa atctgacgat gattacaagc aattcattga aagagtctca caactatttg
      661 cctcaaaaaa aatcaccaaa agtaaaaatg ataagctttt gatgagtggg gaatctatac
      721 cagatgactt tatcaacagg caacttaacg aaacaaggtt tatttcaaag gaatccatca
      781 atttattaaa aaatatctgc aggaatgttc ttgtctcatc tggttcagta actgaatttc
      841 taagaaggga atggggctat aacgaagtgc tcaaacaact aaattggcac aagtatgaaa
      901 aagcaggctt aattagcaat gataagattg aaggttggag taagcgtgac gaccacaggc
      961 atcatgcaat agatgcactg gttgtagctt gcacaaaaca atcatttata caaaaactca
     1021 atacattgaa tagcagcctt acccgagaag aaatgtatca aagggtaaaa ggcaatgctc
     1081 cggaaaaatg gcagggcagg aaacctctga tggaacagta ttttcaactt gaacagcctt
     1141 ttacaaccca ggaagtaaaa gaggctgttg acaatatact catatcccta aaacctggaa
     1201 aaaaagtagc tactaagtct gttaatcgaa aaacaggtaa ccagatcaca cttactccga
     1261 gaggtcaact acataaagaa caggtctatg gaaaaatcag aaaatattca gctaagaaaa
     1321 gcccattgaa tgcaaaattt attcatgctg aattaattgc agacccaact gagaaatcac
     1381 ttgtcatcaa caggctaaaa cagttcgata acgaccccaa aaaagccttt aaggatttgg
     1441 aaaaaaatcc tatttggatc gatagtggaa aaactnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nttgtgggaa gatcattttg tatataaata cacgcttgat cagaatttca
     1561 aagaaaaaga tattgaattt atagttgaca ttggcatcaa ggaaaaagtt cgccagagat
     1621 ttatagaaaa aaaagacact ggcgaacatc cattaaaaaa ccttgaacag gaacccatct
     1681 ggctgaataa agaaaagaat attccgatca aatcggtgcg atgctttacc ggattaactg
     1741 aacttgtgcc acttcataca gcagaaaatg ggagaacctt acctgcaagt aagaaaaccg
     1801 aaaacagcaa acctgttgat tatgtgagca caagaaacaa tcatcatatt gcgatatacc
     1861 aagacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnngt attgaaaacc aggaattgct taataatatt cctcaaaacg
     2041 actggcagtt tttcttaagt atggctcaaa atgagttgtt tgtcgtttct aaaacattgg
     2101 aagaaattaa aaatgccatt aaagaaaata aacttaatga actttcaact caattgtata
     2161 gaatacaaaa gttgtctgaa actgattatt atttccgcca tcatcttgaa accaaattag
     2221 aaaaaaactc aaatgaagaa aagcaatcca tgtttcttgg gaaagtgata aggataagga
     2281 gtcttaaagc gcttaaagaa ctacaactac ataaagttca aataaattct attggaaaaa
     2341 taaaattggt caattaaaaa tgatcaaaaa agtactatac tttggcaacc cggcttacct
     2401 gaaattcagg aatgagcagt tactgattga atcagatgct gatgatcagg aatccgggca
     2461 gcgttcggtt cctgttgaag atattggcct ggttattctt gaccatagcc agattaccat
     2521 aacgcaggta accattgcca aattgctgga aaacaatgcg gcgctgatta cctgtgatgc
     2581 cacccatcac ccggttggga tgatgctgaa tttagatggc aataccctgc aaagccagaa
     2641 acaccaggtg caggttgatg cttcacagcc actgaaaaag cagctctggc agcaaaccgt
     2701 gacagcgaaa atccggaacc aggccgccct gctgaaaaaa gaaaaggcgc cttccggata
     2761 tctcgaatcg ctggccgaaa aggtgaagag tggcgacagt gaaaatgccg aagccaaagc
     2821 cgctgcctat tactggaaac gattattccc ggatttcctc gatttttccc ggagccggta
     2881 tggcccaccc ccgaataacc tgctgaatta tggttatgcc atcttgcggg cgctggtggc
     2941 ccgtgcactg gtcagcagtg ggctgctccc caccctgggc atttttcatc ggaaccagta
     3001 caatgcctat tgcctggccg atgatatcat ggaaccttac cggccttatg tggaccaggt
     3061 ggtttgccag attgtacgaa tgaacggtaa attcctggag atgacggcca gcatgaaaaa
     3121 agacctgttg tccattccgg ccatggatgt ggtattgcag ggcagaaaaa gcccaatgat
     3181 gaacgctgtg agccggacaa cggccagcct ggtggcctgt tttgagggtg aatcaagaaa
     3241 aatcctctat cctgaactgg tatgacatga aatcatatta tgaacggatc aatgcctatc
     3301 ggatcatgtg ggtgctcgta atgttcgacc ttccgactga aaccaaggcc gaccggaagc
     3361 ggtacgccct tttccgaaag aaaatcctgt cggacgggtt ccaaatgttc cagttcagta
     3421 tgtatatccg gcattgttcc agcaaggaaa atgcggaagt gcatcaaaaa agggtcaaaa
     3481 aaatgttgcc gcccaaaggg catgttggta tcatgatcat caccgataag caatttggta
     3541 tgatggagat atttcacggt aaagaccctg ccccgacacc tcccaccgcg cagcaattag
     3601 aattctttta attgcaccct tagaaataaa aaactggttt ccggatgggg atttttaatc
     3661 ctaaaaaagt cccgaaaacc agtcccaaca agcttttcag cgaaattaga tgttggtggc
     3721 cgaagctaac caaaaaattg aaagcaaatc acaaccgttc cgctctaatc ctgacctttc
     3781 aatgagatgt tggtggccga agctaaccaa aaaattgaaa gcaaatcaca actataattg
     3841 ttttgaaatc ttatgctctc cagatgttgg tggccgaagc taaccaaaaa attgaaagca
     3901 aatcacaaca tatcgtcggt tacttcgatg ccgagctttg atgttggtgg ccgaagctaa
     3961 ccaaaaaatt gaaagcaaat cacaacccaa cagc
//
LOCUS       DAORLS010000277         3973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12407_length_3973_cov_1.642164, whole genome shotgun sequence.
ACCESSION   DAORLS010000277 DAORLS010000000
VERSION     DAORLS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12407_length_3973_cov_1.642164"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK977_16290"
     CDS             <1..865
                     /locus_tag="PK977_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19736.1"
                     /translation="GLTNAQRGVFDFGTLKAEGSFGRQIGFGNSQDAVLNSTLNLQLS
                     GMLGDSIELQAAITDNNIPIQPDGNTQQLNEFDQVYLQFRKKGWQLNLGDIDLRQNQS
                     YFLNFYKRLQGISFQTTNKISKTAESKTLVSGSVAKGKFTRNILEPLEGNQGPYRLRG
                     ANNEFFFIILANTERVFYDGQLLQRGEDQDYVINYNTAEITFTPKRMITKDSRIQVEF
                     EYADRNYLNANLYLAQEFDLNKKVKVRIGAFNNNDAKNSQINQILDPRQKQFLADLGD
                     SGLVARGQVAG"
     gene            831..3143
                     /locus_tag="PK977_16295"
     CDS             831..3143
                     /locus_tag="PK977_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19737.1"
                     /translation="MVSSPGVRSLGDSVQRAFYPSAIVDTFAAGKILYEKVYIGLDSF
                     YRYSTDPQLAKYNLSFADVKQGNGNYVPDFNGANGKVYRYVPPVGNVKQGQYEPVQIL
                     VAPRLQQIINLGVDYTIDKNTLVKAEVATSNNDVNTFSSKDNGDDRGWAAKFQISNEK
                     VLRQSKQLQLVTGLDYEYVQHKFRPLERLRTVEFTRDWGLNILAVPVPVDENILRAKV
                     GIKDKSAHSFSYQFTNYHRSDDYNGFQNMLQHNANWKGWQFNNQFTLTSFNALNDKGT
                     FLRPTIDISKQLKKLDNWRIGFNYSLEQNNNRHKLSDTLNPLSFNFDIYSAYLKSDES
                     KQNRYRITFFTRSDKYAVGKDFVRGDRSLNLNLQTELLKNQNRQLYINTTFRKLKVYN
                     ATVSKQKEDETILGRAEYIMNEWKGLLSGNVLYEVGAGQEQRRDFAYLEVPAGTGQYA
                     WIDYNSDGVQQLNEFEIAAFPDQAKFIRIFTPTNEFIKANYITFNYSLNINPRSVLSS
                     SDLKGFKKLLSKIILITSLQTNKKSIAKGVFEFNPFKYGISDTALVTLSTTILNTISF
                     NRFNSKWGVDITNLRNNGKSLLTYGYETRRLNDWQSRWRWNISRSLQFTLNARKGINA
                     LYTPQFANRNFELSIYNAEPNLVFISGTTFRVSGGYKFEQKKNLPLYGGEKSVSNAVN
                     VESKYNIPQSSSITGKFTLNSINYDFPANTTVSYIMLDGLLPGKNYLWSLGLNKRLAN
                     NLEISLQYDGRKAGTSRTVHLGRAAISALF"
     assembly_gap    3178..3279
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3280..>3599)
                     /locus_tag="PK977_16300"
     CDS             complement(<3280..>3599)
                     /locus_tag="PK977_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRF19738.1"
                     /translation="DALKEAIKNENAQLIIMNNNIEEQIAGMESQEDKLLFMEEYKME
                     EPALDRLIHSAYKLLNLYTYFTAGVQEVRAWTIHQGWKAPQAAGVIHTDFEKGFIKAE
                     VIAY"
     assembly_gap    3600..3663
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3664..>3973)
                     /gene="ychF"
                     /locus_tag="PK977_16305"
     CDS             complement(<3664..>3973)
                     /gene="ychF"
                     /locus_tag="PK977_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625808.1"
                     /note="EngD; translation-associated GTPase; the crystal
                     structure of the Haemophilus influenzae YchF protein
                     showed similarity to the yeast structure (PDB: 1NI3);
                     fluorescence spectroscopy revealed nucleic acid binding;
                     the yeast protein YBR025c interacts with the translation
                     elongation factor eEF1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRF19739.1"
                     /translation="VDAIIHVIRCFEDENILREEGAINPVSDKEIIDTELQLKDLESV
                     EKKMQKTEKQIKLDPKYKTELEILSRCKEHLEKGKNIRELELDKEEKVAIADLFLLTD
                     K"
BASE COUNT         1156 a          764 c          788 g         1099 t
ORIGIN      
        1 tggattgacc aatgcacaac gtggtgtatt tgattttgga acattgaaag cagaaggaag
       61 ttttggcagg caaatagggt ttggcaatag ccaggatgcg gttttaaatt ctacattaaa
      121 cctgcaacta agcggaatgc ttggtgacag catagaatta caggctgcca ttactgataa
      181 taatatcccg attcagccgg atggaaatac gcaacaatta aatgaatttg accaggtcta
      241 tcttcaattc cgaaaaaaag gctggcagct taacctgggt gatattgacc ttcggcagaa
      301 ccagtcttat tttttgaatt tttataaaag attgcagggt atttcttttc agacaaccaa
      361 taaaatctcg aaaaccgctg aatccaaaac actcgtcagt ggttctgttg ctaaaggaaa
      421 atttactcgc aacatactgg aacctttaga agggaaccag ggaccttacc ggttaagggg
      481 tgccaacaac gaattcttct tcataatact tgccaatacc gaaagagttt tttatgacgg
      541 acaattgttg caaaggggcg aagaccagga ctatgttatc aattacaaca cagcagaaat
      601 tacattcacc ccgaaaagaa tgatcactaa ggatagccgc atacaggtag aatttgaata
      661 tgctgacagg aattacctta atgctaatct ttacctggcc caggaatttg acctgaataa
      721 aaaagtaaag gttcggatag gtgcatttaa taataatgat gcaaaaaatt cccagatcaa
      781 ccagatattg gatccaaggc aaaaacagtt cctggcagat ctgggtgaca gtggtctcgt
      841 cgccaggggt caggtcgctg ggtgatagtg tgcaaagggc tttttatccg tcagccattg
      901 tagacacttt tgcagccgga aaaattcttt atgaaaaagt ctacatcggg cttgactctt
      961 tctatcgtta ttcaaccgat ccgcagttgg ccaagtacaa tctttcattt gctgatgtca
     1021 agcagggcaa tggaaattat gttcctgact ttaatggcgc caatggtaaa gtgtatagat
     1081 atgttccgcc tgttggtaat gtgaagcaag ggcaatatga accagtgcag attcttgtcg
     1141 ccccccgcct gcagcagatc attaacctcg gtgtggatta taccattgat aaaaacaccc
     1201 tggtaaaggc cgaagtggca acttctaaca atgatgtcaa cacattttca tcaaaagata
     1261 acggtgatga caggggctgg gcagccaaat ttcagatcag taatgaaaaa gtacttcggc
     1321 aatcaaaaca attacagtta gtaacgggac ttgattatga atatgtgcaa cataagtttc
     1381 gtcccctgga gaggttgcgt accgttgaat ttacccgtga ctgggggctt aatattctgg
     1441 cagtgcctgt gccggttgat gaaaatatat taagggctaa agttggtatc aaagataaat
     1501 cagctcacag cttctcttac cagtttacca actatcaccg cagcgacgat tataacggat
     1561 ttcaaaatat gctccagcat aatgcgaact ggaaaggatg gcagttcaat aaccagttta
     1621 cacttaccag ttttaatgca ttgaatgata aaggaacgtt cctgagacca actattgata
     1681 tcagcaagca attaaaaaaa ttggacaact ggcggattgg gttcaattat tcactggagc
     1741 aaaacaataa ccggcacaag ctgtccgata cactcaatcc gctgtctttt aattttgata
     1801 tttattctgc ctacctcaaa tcagatgaat ccaaacaaaa ccgttaccgg atcacttttt
     1861 ttacacggtc tgataaatat gcggtgggta aggattttgt aagaggagac cgaagcctta
     1921 acctgaacct gcagacagaa ttactcaaga accaaaacag gcaattatat atcaatacaa
     1981 cttttagaaa gctgaaagta tataatgcca ctgtttcaaa gcagaaagaa gatgaaacga
     2041 tactgggccg tgcagaatat ataatgaatg aatggaaagg gttgttaagc ggaaatgttt
     2101 tgtatgaagt gggggcaggg caggagcaac ggcgtgattt tgcctatctc gaggttccgg
     2161 cgggtaccgg acaatacgcc tggatcgatt acaacagtga tggggtacag cagcttaatg
     2221 aatttgaaat cgcagcattc cctgaccagg ccaaattcat ccgcattttt acacctacca
     2281 atgagttcat taaagctaat tatatcacct ttaattatag cctgaacatc aatccgagat
     2341 cagttctaag ttcttcggat ttgaaagggt ttaagaagtt gctttcgaaa atcatcctga
     2401 tcacttcctt gcagacaaac aaaaaatcaa tcgcaaaagg tgtttttgaa tttaatccat
     2461 ttaaatatgg tatatcagat acagcgctgg ttactctctc cacaaccatt ctaaatacga
     2521 tttcatttaa ccggttcaac agtaaatggg gtgtagatat tacaaacctg aggaataacg
     2581 ggaagtcgct tctgacgtat ggttatgaaa cccggaggct caatgactgg caaagccgtt
     2641 ggcgctggaa tataagccgg tcattgcagt ttaccctcaa tgccaggaaa ggaatcaatg
     2701 cactttatac gccgcaattc gcaaaccgga attttgaatt atccatttat aatgccgaac
     2761 ctaaccttgt tttcatcagc gggactactt tccgggtttc aggtggatac aagtttgaac
     2821 aaaagaaaaa cctgccatta tatggcggtg aaaaatcggt ttccaatgct gtgaatgttg
     2881 aatcaaaata taatattccc caaagcagtt cgattaccgg taagtttacg ctaaacagta
     2941 tcaactatga ttttccagct aatacaacgg taagttatat tatgctggat ggtttattgc
     3001 ccgggaaaaa ttatttatgg tcattggggc tcaataaaag gctggccaat aacctggaga
     3061 tcagccttca gtatgacgga agaaaagcag gaacttcccg tacagtacat ttgggaagag
     3121 ccgctatttc agcattattc taaacattaa agcggaaatg catcacatct ccgtcttnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng taagcgatca cttcggcctt
     3301 aataaatcct ttctcaaagt ctgtatgaat tactccggct gcctgtgggg ctttccatcc
     3361 ctggtggatg gtccaggccc ttacttcctg aacaccggca gtgaaataag tatacagatt
     3421 aagtaattta taagcagagt gaatcagcct gtccagcgcc ggttcttcca ttttatattc
     3481 ctccatgaat aacagtttgt cttcctggct ttccatccct gcaatttgtt cttcgatatt
     3541 attattcatg atgataagct gtgcattctc atttttgatg gcttctttta aggcatctgn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnngtttgtc tgtaagcagg aaaaggtcgg ctattgcaac tttttcttct ttatcaagtt
     3721 ccagttccct gatattcttg cccttttcaa gatgttcttt acaacggctt aatatttcca
     3781 gttcggtttt atattttggg tccagcttta tctgcttttc tgttttttgc atcttcttct
     3841 ctacactctc caggtctttc aattgaagtt cagtgtcaat gatctcttta tcgcttacag
     3901 ggttgatagc gccttcctcc cgaagtatgt tttcgtcttc aaaacaacgg ataacatgta
     3961 ttatggcatc aac
//
LOCUS       DAORLS010000278         3971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12419_length_3971_cov_1.852911, whole genome shotgun sequence.
ACCESSION   DAORLS010000278 DAORLS010000000
VERSION     DAORLS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12419_length_3971_cov_1.852911"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>364)
                     /locus_tag="PK977_16310"
     CDS             complement(<1..>364)
                     /locus_tag="PK977_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRF19740.1"
                     /translation="MKREGWTPGFINQKIDEYVADLPNRDEFVYKRKYKEFNAGDLKK
                     DKIEEEKERMEKLRAAVNEFDHFQQLMRKKNRYDFDDMINWVIKAFEENKNLLANYQE
                     KFQYILVDEYQDTSGTQNR"
     assembly_gap    365..380
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<381..861)
                     /locus_tag="PK977_16315"
     CDS             complement(<381..861)
                     /locus_tag="PK977_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRF19741.1"
                     /translation="MSFNKERLERKFAEEFEKLNEKQQLAVNNIEGPVMVIAGPGTGK
                     TQILASRIGKILLDTDALPENILCLTYTDAGVVAMRKRLLQFIGPDAYKVNIYTFHAF
                     CNDVIQDNLSLFEKTALDPISDLEKIQLFKALIDSFPKNHLLKRYRGDVYFEVNNLQQ
                     "
     gene            923..1420
                     /locus_tag="PK977_16320"
     CDS             923..1420
                     /locus_tag="PK977_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF19742.1"
                     /translation="MPAHSIKTVQKIPVSLEKAWEFFSNPANLQAITPGDMGFKIISK
                     HHGEKMYAGQLIEYTVKPVLGIPVYWMTEITQVKDRQYFIDEQRFGPYALWHHQHHFR
                     EIDGGVEMTDIIHYKNPLWFLGKLANTLFVKNKLNNIFNYRFQKVEELFGKWPGGQPI
                     QVEIN"
     gene            complement(1445..>1931)
                     /locus_tag="PK977_16325"
     CDS             complement(1445..>1931)
                     /locus_tag="PK977_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF19743.1"
                     /translation="EESQRHHILGLQANLWTEQVYNMRHAQYMTWPRAFAIAEALWSP
                     KEKRNWNDFVPRVERHFERFEQAQVKYAPSIYDPIVTITKKDTATVVVKLETEIEGLS
                     IHYSFDNSFPDNFYPVYKEPLMVPKEAAAIKMITYRNNKPIGRLLVIPMADLKKRAGI
                     K"
     assembly_gap    1932..1941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1942..>2202)
                     /locus_tag="PK977_16330"
     CDS             complement(<1942..>2202)
                     /locus_tag="PK977_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF19744.1"
                     /translation="KKMIGWDEILQGGLAPNAAVMSWRGMKGGIEAAKMGHEVVMSPT
                     DFAYIDYMQGDASIEPPVYATLRLKKAYQFEPLPNGADAKLI"
     assembly_gap    2203..2328
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2329..3405)
                     /locus_tag="PK977_16335"
     CDS             complement(<2329..3405)
                     /locus_tag="PK977_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRF19745.1"
                     /translation="MKKSLLLLLVSFFSLWCFAQRTSETTISLIPQPVSIVQGTGTLK
                     LPFSLTIITDKNEEVKRIATDLSRRLKTAANKVTVKEGKILLPKSIFLSLTNETGMPK
                     EGYRLKVTATGISLTAIEPAGIFYGVQTLLQLFPKQIESPEIQKPVNEWALPVVTIED
                     HPRFGWRGLMLDVSRHFFTVAQVKDYIDQMVKYKFNLLHLHLTDDQGWRIEIKSLPKL
                     TEVGAWRVERTGTFGTFPKPQPGEKATYGGFYTHEDIKELVKYAAERYVNILPEIDVP
                     GHSLAAVASYPELSCTPGEYYVSPGDRFMVWPGGGQHFYGLLDNTICPANEKAYEFLD
                     KVFTEVAQLFPFPYIHMGGDETARN"
     gene            complement(3510..>3971)
                     /locus_tag="PK977_16340"
     CDS             complement(3510..>3971)
                     /locus_tag="PK977_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19746.1"
                     /translation="GDMEGAEKVLNTIKFPNLLYKPIRSVYYTLKGNIAMVKQDFDGA
                     EKMMKKGLDLGMPMKEAEGASLLQMGMLAMQKNDLKQAESYIRSAIRKGLPDKENESA
                     AYLQMCSLMMNKREFRAAKDFFRKAKALKPTTPQIVDQIKQIEKYITRIPG"
BASE COUNT          964 a          861 c          774 g         1220 t
ORIGIN      
        1 gccgattttg tgtgccactg gtatcctggt actcatccac cagtatgtat tgaaattttt
       61 cctggtaatt cgccagcagg tttttgtttt cttcaaatgc tttgatcacc cagttgatca
      121 tatcatcaaa atcatagcgg ttcttcttcc gcatcagttg ctgaaaatgg tcaaactcat
      181 tcactgctgc ccgcagcttt tccattcttt ctttctcttc ttcgatctta tcttttttca
      241 ggtcgcctgc attgaattct ttgtattttc ttttgtaaac aaattcatcc ctgttgggca
      301 ggtcggcaac gtattcgtca attttctggt tgatgaagcc gggtgtccag ccttcccgtt
      361 tcatnnnnnn nnnnnnnnnn actgctgcag gttgtttact tcaaagtaca catcaccgcg
      421 gtatcgtttc agtaaatgat ttttgggaaa tgaatcaatc agcgctttga acaactgaat
      481 tttttcgaga tctgaaatgg ggtccaatgc tgttttttca aagagggaaa ggttgtcctg
      541 tatcacatcg ttgcaaaagg catgaaaagt atagatgttc actttgtagg catccggccc
      601 aataaactgc aataatctct ttcgcatggc caccacaccg gcatctgtgt aggtaaggca
      661 aaggatgttt tccggcaatg catctgtatc cagcaatatt ttgccaatgc ggctggcgag
      721 tatctgggtt ttaccggtgc ccggccccgc aattaccatg acgggccctt cgatgttatt
      781 gacagccagc tgctgttttt cattcagctt ttcaaattct tcagcgaact ttctttctaa
      841 tctttctttg ttaaaggaca tttttatctg aacttttttc tctctgacag gttattccaa
      901 cgaatttaaa tcaataatct gcatgccggc acattctatc aaaactgtac aaaagatccc
      961 tgtttcttta gaaaaggcat gggagttttt ctccaatcct gccaacctgc aggccattac
     1021 cccgggcgat atgggcttta aaatcatttc caagcatcac ggtgaaaaaa tgtatgcggg
     1081 tcagttgatt gaatacacag taaaaccggt tttgggcata cctgtttact ggatgacaga
     1141 aataacccag gtgaaagaca ggcagtactt tattgatgag caacgttttg gcccctatgc
     1201 gttatggcac caccagcatc atttcaggga aatagacggc ggtgttgaga tgactgatat
     1261 tattcattat aaaaacccgc tgtggttttt gggcaaactg gccaatactt tatttgtgaa
     1321 gaacaaattg aataatattt tcaattatcg gtttcaaaag gtagaggaat tgtttggcaa
     1381 atggcccgga ggccagccca ttcaggttga gataaattaa tagctactga ctacaggctt
     1441 cccactactt aatccctgct ctttttttca agtctgccat cggtataacc aataaccgtc
     1501 caatcggctt attatttcgg taagtgatca ttttaatggc agcagcttcc ttcggaacca
     1561 tcagcggctc tttgtaaacg gggtaaaaat tatcggggaa agaattgtcg aaactgtaat
     1621 ggatagacag cccttcaatt tcagtttcca gcttaaccac taccgtagct gtatcttttt
     1681 tggttatcgt tacaatagga tcatatatac tgggagcata ttttacctgt gcctgctcaa
     1741 accgttcaaa atgacgctct actcttggca caaaatcatt ccagttcctc ttttctttcg
     1801 gactccacag cgcttctgca atggcaaaag cccttggcca ggtcatgtat tgagcatggc
     1861 gcatattgta cacctgttcg gtccacaggt tggcctgcag gccgaggatg tggtggcgct
     1921 ggctttcctc cnnnnnnnnn nttgataagc ttagcgtcag ccccattcgg aagcggttca
     1981 aactggtaag cttttttcag ccgtagtgta gcataaaccg gtggttcaat agaggcatct
     2041 ccctgcatat aatctatgta agcaaaatca gtaggactca tcactacttc atgtcccatc
     2101 ttcgctgctt caattcctcc tttcattccc cgccagctca ttacagcggc attgggggca
     2161 aggccaccct gcaatatttc atcccagccg atcattttct ttnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ttcttgctgt
     2341 ctcatcaccc cccatatgga tgtaaggaaa gggaaacagt tgcgccactt cggtaaatac
     2401 tttatctaaa aattcatatg ctttttcatt agccgggcaa atagtgttat ccagcagacc
     2461 ataaaagtgc tgaccgcctc ccggccatac cataaaccgg tctccggggc ttacataata
     2521 ttcgccgggt gtgcaggaca actcgggata agaagccaca gcagcaaggc tatgtcccgg
     2581 cacatcaatc tcaggaagga tgttgacgta tctctctgca gcatatttca ctaactcctt
     2641 aatgtcttca tgagtgtaga aacctccgta agtagctttt tctcccggtt gtggtttggg
     2701 aaaagttcca aaagtgcctg ttctttctac ccgccatgcc cccacctcag taagtttagg
     2761 taagctttta atttcaatgc gccagccctg gtcatcggtg aggtgcaggt gcaataaatt
     2821 gaatttatac ttcaccatct gatcaatata atccttcacc tgtgctacgg taaagaaatg
     2881 acgggataca tccagcatca aacctctcca gccaaagcgg ggatgatctt cgatagtgac
     2941 tactggcaaa gcccattcgt taaccggttt ttgtatttcc gggctttcaa tttgttttgg
     3001 gaataattgc aggagtgttt gaacaccata aaaaattcct gcaggttcaa tagcagttaa
     3061 agagatacct gtagcggtta cttttaatcg gtatccttct ttgggcatac cggtttcatt
     3121 ggtcagggat aaaaaaattg attttggtaa caggattttt ccttctttca ccgttacttt
     3181 atttgcagct gtttttaacc tgcgggaaag atcagttgct atccttttta cttcttcgtt
     3241 tttatctgtg ataatagtaa gactaaaagg aagctttaaa gtgccggtgc cctgtacaat
     3301 cgatactggt tggggtataa gagaaatggt agtttcagaa gttctctgag caaaacacca
     3361 taaagaaaaa aaactgacta aaagaagtaa taacgatttt ttcatatgct ttgttattac
     3421 tggcaagtta cttcattcaa ctacctgaaa taaaaaaagg ttgacagcta ttcttgtcaa
     3481 cctttaaact tttaagacta tcaaccgtat tatcccggta tcctcgtaat atatttttca
     3541 atctgtttga tctggtcaac gatctgcggg gtagtgggct ttagtgcttt tgcttttctg
     3601 aagaaatctt ttgctgccct gaattccctt ttgttcatca tgaggctgca catctgcagg
     3661 taagcggctg attcgttttc tttatcgggc aatccttttc ggatggctga cctgatatag
     3721 ctctcagcct gtttcaggtc gtttttctgc atggccagca ttcccatttg cagcaggctg
     3781 gcaccttcag cttctttcat cggcatgccc aggtcgaggc cttttttcat cattttttct
     3841 gctccgtcaa aatcctgttt taccatagcg atattgccct ttagggtata atacacggag
     3901 cggataggct tatacagcag gttgggaaac tttattgtat tcagtacttt ttcagcgcct
     3961 tccatatctc c
//
LOCUS       DAORLS010000279         3950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12492_length_3950_cov_2.082670, whole genome shotgun sequence.
ACCESSION   DAORLS010000279 DAORLS010000000
VERSION     DAORLS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12492_length_3950_cov_2.082670"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="PK977_16345"
     CDS             <1..1704
                     /locus_tag="PK977_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF19747.1"
                     /translation="DGGTVVQLNDDQNKYSVNTGAIANITYVKGRHKVSFKNLYNQLF
                     EDNYYTRSGVNNDRIQDINFRSSVLNQRSLYSGQLEGDHQLTTSSIKLRWNGNFAYNW
                     KSQPDLRTLSYNRGKGTTDPFEYNDDDTRRFFSSLKDYSYGANGSISIPFTFNKDKQT
                     FKAGGSTLIRIRDFRSRIFQYVPASATQFDQSKETLPFDQIFAPQNIGIDGFVLNDFT
                     NNQDKYFGVSVLNGMYGMFDNKFGEKIRLVWGVRVENFQQFLTTKDVTSKRIVVNTEK
                     WDVLPSFNFTFAPNTKNSIRLAASRTVARPEFREIAPFSFYDYEANYGVNGNVDLQRS
                     SILNGDIRYEFYPKGGEAITFGAFYKFFNDPIELRLDPSSVLDRRNYGYSNADEAYTL
                     GAEFEVRKNLDFLGESFTNFSTFANLTYIYSKVTLQGSSTTTRPLQGQSPYLINLGLQ
                     YNSKQGGWNGSLLYNRIGQRLALVGISELGFPDVYERPRDQVDFQLAKKILNNKAELK
                     LTWADLLNPAYYFYENVDTKKAFSSGSDRLFNSYRPGSTITIGFTYDFNIGKNNAFTK
                     K"
     gene            1730..>2420
                     /locus_tag="PK977_16350"
     CDS             1730..>2420
                     /locus_tag="PK977_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19748.1"
                     /translation="MKKILFGALTLITLFGATSCVKVNFDEEQVNTPTPEPTSNIIQG
                     TISSSRFYAKGTWILKGYVYVTEGATITFEAGCIIKSDITEKGALIIERGAKLIASGT
                     PANPIVFTSGKAVGSRAPGDWGGIILLGKAPTNRPLDPAPTIEGGVGRQYGGTDPLDE
                     SGILRYVRIEYAGIAAEPGSEINGLTCGGVGSGTIIENVQVSFGNDDAFEFFGGTVNA
                     KNLVAFATADDD"
     assembly_gap    2421..2634
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2635..>2849
                     /locus_tag="PK977_16355"
     CDS             <2635..>2849
                     /locus_tag="PK977_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19749.1"
                     /translation="HNYSNRWRRATQFILRNSILVGHPDAGFSLESDATLNDYYVNNI
                     SEFKNNLVHASTNVYRSSNNAIATAVQ"
     assembly_gap    2850..2882
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2883..3099
                     /locus_tag="PK977_16360"
     CDS             <2883..3099
                     /locus_tag="PK977_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19750.1"
                     /translation="ITLANSAAAQLTSPFYSTSPNFLPVTGSPALTGASFSGMNPFFT
                     STTYRGAMGTTNWTSGWCNWDPQNAAY"
     gene            3176..3616
                     /locus_tag="PK977_16365"
     CDS             3176..3616
                     /locus_tag="PK977_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19751.1"
                     /translation="MLRFVLASIVIVSAVLLSCKNETGKKNLLPALADSDSAIVMYYH
                     TPGNPRFFNMTKVYDKAVIARFSETVNKKTIDSKNNCTTQGKIYFYGKKGAVETIYFS
                     RADTCMTLSFIKTGEKYFVKMNKELKLSLDELEKKAITLPAAGE"
     gene            complement(3617..>3950)
                     /locus_tag="PK977_16370"
     CDS             complement(3617..>3950)
                     /locus_tag="PK977_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19752.1"
                     /translation="DNQQFGGEKRLSPEQTLLFEQSDCDDRAALFFYLVKEIYNLPMI
                     VLSYPEHVTVAVKFDKPVGKPIFYNGEKYSVCEPTPQKEDLAVGELLPSLLKQQYEVV
                     YAYHPSGQ"
BASE COUNT         1068 a          798 c          805 g         1032 t
ORIGIN      
        1 gatggaggca cagtagttca gttgaatgac gaccagaata aatacagtgt gaatacaggt
       61 gctattgcca atatcactta tgtaaaagga agacataaag tatcgttcaa gaacctgtac
      121 aaccagttat tcgaggacaa ttattataca aggtctggtg taaacaacga ccgtatacag
      181 gatatcaact tccgttcttc ggtattgaac cagcgttcac tttatagtgg ccagttggaa
      241 ggtgatcacc aacttactac aagtagtatt aaactaaggt ggaacggaaa ctttgcttat
      301 aactggaaat cacaacctga ccttcgtacc ctttcttata accgtggcaa aggcactaca
      361 gatccttttg aatataatga tgatgatacc cgtcgcttct tcagcagttt gaaagattat
      421 agctacggag ccaatggttc tatcagtatt ccttttactt tcaataagga taaacaaaca
      481 ttcaaggcag gtggctctac cctgatccgt atccgtgatt tccgcagccg catcttccag
      541 tatgtaccgg cttcggccac acagttcgat cagagcaaag agaccttacc gtttgaccag
      601 atatttgctc cgcagaatat cggtattgat ggttttgtgc tgaacgactt taccaataac
      661 caggataaat attttggtgt atctgtgctg aatggcatgt atggtatgtt cgacaacaag
      721 tttggcgaaa agatccgttt ggtatggggt gtaagggtgg agaatttcca gcagttctta
      781 actacgaaag atgttacctc taagcgtata gtggtgaata cagaaaaatg ggatgtattg
      841 ccttcgttta atttcacctt cgctcccaat acaaaaaata gtatccgcct cgcagctagc
      901 cgtacggtgg ccaggccgga gttcagggaa attgctccgt tctccttcta tgattacgag
      961 gctaactacg gcgtaaatgg taatgtagac ctgcagcgca gttctatact caacggtgat
     1021 atccgctacg agttttatcc caaaggcggc gaagccatca cattcggtgc tttctataaa
     1081 ttctttaatg atcccattga attgcgtctc gatccttcca gcgtactcga cagaagaaat
     1141 tacggatatt ccaatgctga tgaagcctat acattgggag cagaatttga agtaagaaag
     1201 aacctggatt tcctgggaga gagctttacc aacttcagca cttttgctaa ccttacctat
     1261 atctattcaa aagtaacgct gcagggttct tctactacaa cacgacccct gcaggggcaa
     1321 tcaccctatc tcatcaacct gggcctgcaa tataacagca agcagggcgg atggaacgga
     1381 tcattattgt ataaccgcat tggtcagcga ctggcattgg taggtatcag tgaactgggc
     1441 ttccccgatg tgtatgaaag gccaagagac caggtggact tccagttagc caaaaaaata
     1501 ttgaataaca aagcagagtt aaaactcaca tgggctgatt tgctgaatcc tgcctactac
     1561 ttctatgaga atgtagatac gaagaaagct ttcagcagtg gcagcgacag gctgttcaac
     1621 agttacagac ctggctctac catcaccatc ggctttacct atgattttaa tattggcaag
     1681 aacaacgcat ttaccaaaaa ataaaaaaac aaaactcaaa acaagtaata tgaaaaagat
     1741 cctttttggt gcactgaccc tgatcactct tttcggtgct acctcttgtg taaaggtgaa
     1801 ttttgacgaa gagcaggtga atacgcctac gccggaacct accagtaaca ttatccaggg
     1861 taccatttct tccagtcgtt tctatgcaaa gggaacctgg atcctgaaag gatatgtata
     1921 tgttacagaa ggtgctacca tcactttcga agcaggttgt attatcaaaa gcgatataac
     1981 cgagaaaggg gcgttgatca ttgaaagagg cgccaaactg atcgcatccg gtactccggc
     2041 taacccgatt gtatttacca gcggtaaagc tgtaggttca agagctcccg gtgactgggg
     2101 tggtatcatc ctgttgggta aagcacctac taatcgtccg ttggatccgg cacctactat
     2161 cgaaggtggc gtgggccgcc agtacggtgg taccgatccg ctggacgaaa gcggcatcct
     2221 ccgttatgtt cgtattgaat atgctggtat tgctgcagaa cccggttctg agatcaacgg
     2281 tttgacttgt ggtggtgtgg gttctggtac catcattgaa aacgtacagg tatcttttgg
     2341 taacgatgat gcctttgaat tttttggcgg aaccgtaaac gccaaaaacc tggtagcatt
     2401 tgctactgcc gatgatgatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncataac
     2641 tattctaatc gttggagaag agctactcaa ttcattttgc gtaattcaat cctggttggt
     2701 catccggatg cagggttctc attggagtct gacgcaactt taaatgatta ttatgtaaat
     2761 aatatatctg agttcaagaa taacctggtt catgccagta ccaatgttta ccgttccagc
     2821 aataatgcta ttgctactgc tgtgcaaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nntattacac tggctaactc tgctgcagca caattaacca gtcctttcta ttcaaccagc
     2941 cctaattttc tgcccgtaac aggttcacct gctttaacag gtgcaagttt ctcaggcatg
     3001 aatccattct ttaccagcac tacttaccgt ggcgctatgg gtactaccaa ctggacaagc
     3061 ggctggtgca actgggatcc gcagaatgct gcttattaat aaatgagttg aaaaatttag
     3121 aaaacgtcct gtcctatgca ggacgttttc attaatagta aaaaaggctt tttttatgtt
     3181 acgctttgta cttgcgtcga ttgttattgt atcggctgtg ttactttcct gcaaaaacga
     3241 aacgggaaag aaaaacctgc tgccggccct ggcggatagc gacagtgcca tagttatgta
     3301 ttatcatact cccggcaatc cccgtttttt caatatgacc aaagtgtacg ataaagcagt
     3361 cattgcccgt ttctccgaaa cagtcaataa aaagacaata gactcaaaaa acaactgcac
     3421 tacgcagggc aaaatatatt tctatgggaa aaaaggagcg gtggaaacaa tctacttttc
     3481 ccgtgccgat acctgcatga ccttatcttt cattaaaaca ggggagaagt attttgtgaa
     3541 aatgaataag gaactgaaat tgtctttgga tgagctggag aaaaaagcta ttacattgcc
     3601 agctgccggg gaatgatcac tgccctgaag gatggtaagc atacactacc tcgtattgct
     3661 gttttaacag agacggtaat aattctccta ctgccaggtc ttctttttgc ggggttggtt
     3721 cacacaccga atatttctcc ccgttataga aaataggttt gcccaccggc ttgtcaaatt
     3781 ttacagctac ggttacatgt tccgggtacg acagaacaat catcgggaga ttgtagattt
     3841 ctttgactaa ataaaaaaac aaagccgccc ggtcatcaca atcactttgc tcaaataaca
     3901 gggtttgttc gggtgaaagc cttttctcac ctccaaattg ctggttgtcc
//
LOCUS       DAORLS010000280         3940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12531_length_3940_cov_1.757014, whole genome shotgun sequence.
ACCESSION   DAORLS010000280 DAORLS010000000
VERSION     DAORLS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12531_length_3940_cov_1.757014"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>240
                     /locus_tag="PK977_16375"
     CDS             <1..>240
                     /locus_tag="PK977_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF19753.1"
                     /translation="GISRYQYIDSLEFADADAKVFADYLLSNSFWRISKDDITLLVND
                     KAKYGDLTVHLQQLAMQCKPGDNLLFYFSGHGDVET"
     assembly_gap    241..384
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <385..>1437
                     /locus_tag="PK977_16380"
     CDS             <385..>1437
                     /locus_tag="PK977_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19754.1"
                     /translation="TDACRSGKLAGGMKGAEFTAASISAMWKNEIKILSTQPGQLSYE
                     DKKWGNGRGVFSYYLIKGLNGEADANKDSSITLSELEMYVGSNVARETGNKQQPIFEG
                     PNKFSTIIAKISGPVTNSNKQGGNAFSALSKGIILPFDSCNYYYTAMANAIEKNNLIG
                     PGKESATNYYDRLRNCSADNNFLLKANGELLSALMNNAQETVNNSFIGKTLVSYEKFR
                     EGIQVIDQIFRYNDLNLPYMPHWKNLRRYLFVQGEALWNDNRSTFELEKIIDSATAEE
                     PDAAYLFTAKGIVEMRKENWETAIKLLEKSIEKSPGWLIPKYYLGICHSKRFNYRKAL
                     EYYEQVLQKDKSYKTF"
     assembly_gap    1438..1529
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1530..2156
                     /locus_tag="PK977_16385"
     CDS             <1530..2156
                     /locus_tag="PK977_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19755.1"
                     /translation="QFPDHWEPYELLYSYALKKKDSSIAKDFIKRLNVYNDTASMRLL
                     RLRFEHEFYKRPLSIKTLEESILILNDNIDSADYFYTLGLYYEQKNSYDADSAMICYL
                     KAADLDKEEVFYITSLGDFMEKEGMMEELQEILEERITLFTGEDKGQLQELLANSYLA
                     TNDYEKALNICKELLKVGYLKCSDMRKLKKAFKGFTDYEKIMKECKEE"
     gene            complement(2171..>2582)
                     /locus_tag="PK977_16390"
     CDS             complement(2171..>2582)
                     /locus_tag="PK977_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HRF19756.1"
                     /translation="LHKEVFGPYSLVVRCAGMKEMIEVARHLEGQLTATLMATVNDIK
                     ENEELVEAVKNICGRLILNGVPTGVEVCPSMQHGGPFPATTDARFTSVGADAIKRFAR
                     PVAFQNWPDELLPDELKEGNPLGIWRRVNNEFTR"
     assembly_gap    2583..2656
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2657..>2939)
                     /locus_tag="PK977_16395"
     CDS             complement(<2657..>2939)
                     /locus_tag="PK977_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HRF19757.1"
                     /translation="PVFLLPEKIQTEASSVASMYAASVTLGVGQFCTNPGLIIGIESD
                     GLKSFIHDLGKAIQSIAPGTMLHQGIADAYAAKREEALMQENVHLVAESA"
     assembly_gap    2940..2954
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2955..>3300)
                     /locus_tag="PK977_16400"
     CDS             complement(<2955..>3300)
                     /locus_tag="PK977_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF19758.1"
                     /translation="FGASNFPFAYSTAGGDTASAFAAGCPVIVKAHPAHPQTSQIVAD
                     AVLHAAAKCNMPNGIFAHVIGNSFETGKVLVMHPFTKAVGFTGSYSGGKQLFDWANQR
                     KEPIPVFAEMGSV"
     assembly_gap    3301..3310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3311..3662)
                     /locus_tag="PK977_16405"
     CDS             complement(<3311..3662)
                     /locus_tag="PK977_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF19759.1"
                     /translation="MFKDATVEEINIIMQQAWKAYHEYRKFSLKQRAHFMRAIAAELE
                     ECGDALIQTAMRETNLPEARLRNERARTIYQLESFAMACEKGDWLEASIDKAVPDKNP
                     PKPDIRKMLVPLGPV"
     gene            complement(3683..>3940)
                     /locus_tag="PK977_16410"
     CDS             complement(3683..>3940)
                     /locus_tag="PK977_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRF19760.1"
                     /translation="PRETVAIYNLVKNGIIEEATKIYRWFMPLLELDIHPKLVQYIKL
                     AATQTGIGSEYVRAPRLLLEGEERERILKVINDAIKVRPIV"
BASE COUNT         1085 a          779 c          736 g         1005 t
ORIGIN      
        1 ggtatttccc gttaccagta tatcgactcc ctggaattcg ctgatgcaga tgcaaaagtt
       61 tttgcagatt accttctgtc aaacagtttc tggcgaatca gtaaagatga catcacattg
      121 ctggtaaatg ataaagccaa atacggtgat ctcaccgtac acctgcagca actggccatg
      181 cagtgcaaac cgggggataa tcttctattt tatttttctg gacatggcga tgtagagact
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnaacaga tgcctgccga tcgggtaaac tggcaggcgg
      421 aatgaaaggc gctgagttta cagcagcatc tatcagcgct atgtggaaaa atgaaatcaa
      481 aatactaagt acccagccgg ggcaattgtc atacgaggat aaaaaatggg gcaatggtcg
      541 tggtgttttt tcctactacc tcatcaaagg cctgaatggt gaggcagatg caaacaaaga
      601 ctcatccata actttatctg aactggaaat gtatgtggga agcaatgtag cccgggaaac
      661 aggcaataag caacaaccca tttttgaagg gcctaataaa ttctccacaa taattgcaaa
      721 aatcagcggg ccggtaacta acagtaataa gcagggcggc aatgcctttt ccgcattatc
      781 taaaggaatt atactgcctt ttgacagctg taattattat tacacggcga tggcaaatgc
      841 cattgagaaa aataatctca ttgggccagg caaagagtct gcaactaatt attacgaccg
      901 gctaagaaac tgcagtgcag ataacaactt tttactgaag gcaaatggag agttgttatc
      961 tgccctgatg aataacgcac aggaaacggt aaataattcc tttattggaa aaacgcttgt
     1021 ttcatatgaa aagttccggg agggaattca ggtcatcgat caaatttttc ggtataatga
     1081 cctgaacctg ccctacatgc cacactggaa aaatctccgg cgttatttat ttgttcaggg
     1141 tgaagctctg tggaacgaca acagaagtac atttgaactg gaaaaaataa ttgactcagc
     1201 aacagcagaa gagccagatg cagcctacct ttttactgct aaaggcattg tggaaatgag
     1261 aaaggaaaac tgggaaacag ctattaagct gcttgagaaa tctattgaaa aaagtccggg
     1321 ctggctgata ccaaaatatt atcttggcat ttgtcattca aagcgattca attaccgcaa
     1381 agctcttgag tattacgagc aggttctcca aaaagataaa tcatacaaaa catttgannn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc agttcccgga tcattgggag ccatacgagc
     1561 tgctctattc ttatgctctt aaaaagaagg acagttcaat tgcgaaagat tttattaaac
     1621 ggttaaatgt atataatgat acagcttcta tgcggttact aaggctccgg tttgaacatg
     1681 aattttataa aagaccttta tcaataaaaa cactggaaga aagtatcctc atacttaatg
     1741 ataatattga ctctgctgat tacttttata cacttgggtt gtattatgaa caaaaaaaca
     1801 gttatgatgc tgattcagcg atgatctgct atttaaaagc tgccgatctc gacaaagagg
     1861 aagtatttta tattaccagc ctcggggatt tcatggaaaa agaaggcatg atggaagaac
     1921 tacaagaaat actggaagaa aggattactc tcttcaccgg ggaggataag gggcagttgc
     1981 aggaactact agccaactcc taccttgcaa ctaatgacta tgagaaagca ctgaacattt
     2041 gtaaagagct tttaaaagta ggttatctta aatgcagcga tatgcgaaaa cttaaaaaag
     2101 ccttcaaggg atttacggat tatgaaaaaa taatgaagga atgtaaagaa gagtaaaatt
     2161 tctttcacaa ctatcttgtg aattcattat taacacggcg ccatatccct aacggatttc
     2221 cttcctttaa ctcatccggc aataattcat ccggccagtt ttgaaaagcc accggtcttg
     2281 caaatcgttt aatagcatcg gcgccaacag aagtaaacct tgcatccgta gtagcaggaa
     2341 aaggaccacc gtgctgcata ctcggacaca cttccacacc ggtaggtaca ccgttcagaa
     2401 ttaatcgtcc gcagatattt ttaactgctt ccaccagttc ttcattttct ttgatgtcat
     2461 tcaccgttgc catcaatgtg gcggtgagtt gcccttctag atgccgggcc acttcaatca
     2521 tttctttcat accggcacat cttactacta atgaatacgg accaaaaacc tctttatgca
     2581 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnagcc gactctgcta ctaaatgaac attctcctgc atcagcgctt
     2701 cttctctttt tgcggcatag gcatcggcta tcccctggtg aagcatagtg cccggtgcaa
     2761 ttgactgaat ggctttgcct aaatcatgta taaagctttt caatccatca ctttcaatgc
     2821 cgataatgag gccgggattg gtgcagaatt gtcctacacc tagtgtaacg gaagctgcat
     2881 acatagaagc aaccgatgaa gcttctgttt gtattttctc tggtaaaaga aatacaggan
     2941 nnnnnnnnnn nnnncacact gcccatctct gcaaaaacag gaatgggttc tttgcgctga
     3001 ttggcccagt cgaataattg tttaccaccg gaataagaac cggtaaagcc aactgctttt
     3061 gtaaagggat gcattaccaa taccttcccg gtttcaaaag aattaccaat cacatgagca
     3121 aaaatgccgt tgggcatatt gcatttcgca gctgcatgca aaacagcatc cgccactatc
     3181 tgagatgtct gcggatgggc aggatgtgct tttactatta ccgggcatcc ggcagcaaag
     3241 gcgctggccg tatcaccacc cgccgttgaa taagcaaaag gaaaattgct ggcgccaaaa
     3301 nnnnnnnnnn ccacaggtcc cagcggcact aacatttttc tgatatcagg tttaggggga
     3361 ttcttatcag gtacagcttt atcaatgctt gcttccagcc aatcaccttt ttcacaggcc
     3421 atcgcaaagc tttctaactg gtagatcgtt ctagcccgtt cattgcgcaa ccttgcttct
     3481 ggcaaatttg tttccctcat ggctgtttgt atcagcgcat caccgcactc ctctagttct
     3541 gccgcaatgg cccgcataaa atgggcccgc tgtttcaatg aaaactttct gtattcatga
     3601 tatgccttcc atgcctgctg catgattatg ttgatctctt caacagttgc gtctttgaac
     3661 attaaacatt agactttaaa ctttaaacaa tcggtctgac ttttattgca tcattgatga
     3721 ccttaagtat gcgttctctc tcttcccctt ccaataacaa tctcggcgcc ctcacatatt
     3781 cactgccgat accggtttgt gttgctgcca gtttgatata ctgcaccagc tttggatgaa
     3841 tatctaattc cagcaatggc atgaaccagc gatatatttt tgtagcttct tcgattattc
     3901 cattttttac caggttatag atggctactg tttctctggg
//
LOCUS       DAORLS010000281         3900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12703_length_3900_cov_2.350065, whole genome shotgun sequence.
ACCESSION   DAORLS010000281 DAORLS010000000
VERSION     DAORLS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12703_length_3900_cov_2.350065"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>615)
                     /locus_tag="PK977_16415"
     CDS             complement(<1..>615)
                     /locus_tag="PK977_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19761.1"
                     /translation="VKGQWVWNKAGYGNQWTLGEPLRKELQRIMPQAIDGLYATNSIA
                     FGHQSAFYNIKSPATYEHYLMLKKYECLRDYNKLQPESVTGCWVYFVANAMEGLNFPL
                     PGISEIKFSQLESPLWVTHIEIETDAAGNRLLYTSHRTGERIQHCWYFSARPGLPVRK
                     LTRKELYNTYKAHHEKRLNKEIAKFEKLVADDEKKYNSLTPAEK"
     assembly_gap    616..669
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(720..2993)
                     /locus_tag="PK977_16420"
     CDS             complement(720..2993)
                     /locus_tag="PK977_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19762.1"
                     /translation="MLKKIFISLFCLASFIATAQTPLNEEKVKKAVVLMQKMIADPDN
                     MQKIYAELEELKLTKEEKKEAEKRMQQQIMGQLNMDDDMQKKVTGKTTRAEADSLKKQ
                     YEKKQTDEIQKKTGMDKEYISKRIADLDKTVPDKDVQRINALPKKILSDAEIKTLLTT
                     FHNNTLSLVPEFDKKKGEELFNELRSRPNAIIVMGNIANALWLEGANFLSLYLMSKTC
                     MNDGSNVNNMNNYAACLIAYGCEEIALPVLQNLNRKYPGSGTILNNIAQAWFGLGELK
                     MASKYIDSTLVRFPKHSQANFTKSIIEEKEGKTEAAIESAKKSLEEGWSDTKLNHLKK
                     MGYKLRRSDIKHTVPQDPMGLHKFQLPQFPKSYDQALELSPEWDKFKKSCEDEIKKIE
                     AKMEYASKQQYDNEKGRVIIADGMYKKLHDLYSNDDDGWVRKLEKAEEEFEQTIAEIK
                     KLEDAAGKAFEQVSEKFKDKCGEGVDCPAAEICKSTKAVHDQFINQANTLFESMVKNY
                     VNTHRHFFENDVYLYQYSAAPRDFEIYKYQTQRKFLGLLSVIRFPWKGIMTPPDFPGA
                     CDKDKNDGLVKFKGLPDFDEMNCKNTSTLKLLFTEIVTRCNKMKTTFKVDGKLTGVAT
                     LKDVKLEVGFTEDLRNNVTILPAGIESATIEIGVKLGGKEWELGTSKDELKAGVKAEA
                     GVIIEIDKTGITDLGVKTSVSLGGSSSGVPGTGGQGNSGNITLVGAESRWSIKAGPSL
                     KGKSIFKDFTGIKHSVK"
     gene            complement(3001..>3900)
                     /locus_tag="PK977_16425"
     CDS             complement(3001..>3900)
                     /locus_tag="PK977_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19763.1"
                     /translation="FVGSIVTDVTKFKKTNTTQQQPAHTAQNQQNNSGSGITKSTTNF
                     DDGWTATITSGYVKLIKAGTELRLHYIDPSLDDARPNTIDAPEYYWSKYIDPYFTTSN
                     LQKWSGVQYPVIYHMQANAIDKKTGKACFVAIKIVYSGGARPIVVIAPDQNSYLQQFP
                     HPNDIDPMLNANRFAVTAGDIVGTWKGSGGGGVEYYNVYSGTYAGMSAVSSTDEFIFN
                     GNGTYQSTYRSASTNNGGTQFGGQDFKGKFSVTDWTITATNRYQGKTTVYKAQLIAVK
                     GGCLLYMEDSENSSMKYTFYKSK"
BASE COUNT          846 a          841 c          854 g         1305 t
ORIGIN      
        1 tcttttctgc gggtgtgagg ctgttgtatt ttttttcatc atcggccacc agtttttcaa
       61 atttggcaat ttctttattc agtctttttt cgtgatgggc tttgtaggtg ttgtacaatt
      121 ctttgcgggt aagctttcgt accggcaggc cgggccgtgc agaaaaatac cagcaatgct
      181 gtattctttc accggtgcgg tggctcgtat aaaggagccg gttgccggca gcatcggttt
      241 cgatttcgat atgggtcacc catagcggcg attccagctg ggaaaattta atttctgaaa
      301 taccgggcag gggaaagttc aaaccctcca ttgcattggc tacaaaatac acccagcagc
      361 cggttacact ttcgggctgc agtttattat aatccctgag gcattcatat tttttcagca
      421 tcaggtaatg ctcataggta gcagggcttt tgatattata aaaagcggac tggtggccaa
      481 aggcaatgct gttggtagcg tataatccgt caatagcctg cggcatgata cgctgcagtt
      541 cttttcgcag tggttcgccc agtgtccact ggttgccata gccggccttg ttccataccc
      601 actggccttt tacttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnt gtacagtaat gctattgcaa cgatcagttt tttcatagct gttgcttact
      721 tattttacag aatgtttgat accggtaaag tccttgaaaa tactttttcc tttcagggaa
      781 gggccggctt ttatgctcca tcttgattcg gcccccacaa gtgtgatgtt gccgctgttg
      841 ccctgtccgc cagtacctgg cacaccgctg gaagaacctc cgaggcttac agatgttttt
      901 acgccaaggt ctgtaatacc tgttttgtca atttcaatga tgacaccggc ctctgctttt
      961 acgccagcct tcagttcatc tttggaagta ccaagctccc attctttgcc cccgagcttt
     1021 acccctattt caatggttgc actttcaatt ccggcaggca gaatggttac attgttgcgg
     1081 aggtcttctg taaagcccac ttcgagtttt acatctttta aagtagcaac accagtcagc
     1141 tttccgtcaa ctttaaatgt tgttttcatt ttattgcaac gggtaactat ttcggtaaag
     1201 agcagtttga gtgtgctggt gtttttgcaa ttcatttcat caaaatcggg caggcctttg
     1261 aattttacca gtccgtcgtt tttatcttta tcacaggcac cagggaaatc ggggggagtc
     1321 ataatgcctt tccacggaaa gcggatgacg gataacaagc cgagaaactt acgctgggtt
     1381 tggtatttat atatttcaaa atcccgggga gcagccgagt actggtatag gtacacatcg
     1441 ttttcaaaga aatgccggtg agtgtttaca taatttttca ccattgattc aaagagtgta
     1501 ttagcctggt tgatgaactg gtcatgcacg gctttggtgc ttttgcatat ttctgctgcg
     1561 ggacagtcta ccccctcgcc gcatttgtct ttgaattttt ccgatacctg ttcaaaggct
     1621 ttaccggcag catcttccag tttctttatt tctgcaatgg tttgttcaaa ttcttcttct
     1681 gctttctcaa gtttgcgtac ccagccatca tcatcattgc tgtagaggtc gtgcagtttt
     1741 ttatacatgc cgtcggctat tattacccgg cctttctcat tatcatattg ctgtttggag
     1801 gcatactcca ttttagcttc tatttttttg atttcatctt cacagctctt tttgaatttg
     1861 tcccattcgg gagagagttc cagcgcctgg tcgtagcttt tgggaaactg cggcagctga
     1921 aacttatgga ggcccatagg atcttgcgga acagtgtgct tgatatcaga gcggcggagc
     1981 ttgtacccca tttttttgag gtgatttagc ttggtatcac tccatccttc ttcgaggctt
     2041 tttttggctg actcaatagc agcttctgtt ttgccttctt tttcttcaat aatggatttg
     2101 gtgaagttgg cctgtgagtg tttgggaaac cgtaccagtg tactgtcaat gtatttagat
     2161 gccattttca actcgcccag tccgaaccag gcctgtgcga tattgttgag gatggtaccg
     2221 ctgccgggat atttccggtt aaggttttgt aataccggca gggcgatctc ttcgcagcca
     2281 taagcgataa ggcaggctgc atagttattc atattgttta cattactgcc gtcattcatg
     2341 caggttttac tcatcaggta caggctgaga aagttggcac cttcgagcca gagtgcattg
     2401 gcaatgttgc ccattacaat gatggcattg ggacggcttc tgagttcatt aaatagttct
     2461 tcgcctttct ttttatcaaa ctctggcacc aggctgagtg tattattatg aaaagtggtg
     2521 agcagggttt ttatttctgc atcactcaat atttttttcg gaagggcatt gattcgctgt
     2581 acatctttgt caggaacggt tttatccagg tcggcaatcc gcttgctgat atattcttta
     2641 tccattcctg ttttcttttg tatttcatcg gtttgctttt tctcgtattg cttctttaag
     2701 gagtcggcct ctgcccgggt ggtttttccg gttacttttt tctgcatgtc gtcatccata
     2761 ttcagctggc ccatgatctg ctgctgcatt cttttctctg cttccttttt ttcttctttt
     2821 gtcagcttca gttcttccag ttcagcgtat atcttctgca tattatcagg atcggctatc
     2881 attttttgca tcagcaccac ggcttttttc accttttctt cattgagtgg agtttgagct
     2941 gttgctatga atgatgccaa acaaaaaagg gaaataaaaa ttttcttaag cataattgca
     3001 ttatttggac ttataaaaag tgtatttcat agagctgttt tcagaatctt ccatataaag
     3061 caggcaaccg cctttcactg caatgagctg agctttgtac acagtagttt tgccctggta
     3121 gcggttggtg gcagtgatgg tccagtcggt aacagaaaat tttcctttga aatcctgccc
     3181 cccgaattgc gttccgccat tattggtact ggccgaacga taggtgcttt gatatgtacc
     3241 attaccgttg aatataaatt cgtcagtaga agatactgca ctcatgccag cataggtgcc
     3301 actatacaca ttataatact ctacaccacc accgccgctt cctttccatg tgcccacaat
     3361 atcgcctgct gttacagcaa accggttggc gttcagcatg gggtctatat cgtttggatg
     3421 cggaaactgt tgcaggtaac tgttttgatc gggggcaata accacaatgg gtcttgcccc
     3481 tccgctatac acaattttaa tagccacaaa acaagccttt cctgtttttt tgtcaatagc
     3541 atttgcctgc atatgataaa taaccgggta ttgtacgccg ctccattttt gaagattgct
     3601 ggttgtaaaa taagggtcta tatatttgct ccaatagtat tcaggggcat caatggtatt
     3661 ggggcgggca tcatccaggg acgggtcaat atagtgcaac cgaagctctg ttcctgcttt
     3721 gataagtttt acataaccgc tggttatagt ggccgtccag ccgtcatcaa aattggtggt
     3781 tgatttggta atgcccgaac ctgaattgtt ttgctggttt tgtgcagtat gggctggttg
     3841 ctgctgagtg gtattcgttt ttttgaattt tgttacatct gttacaatgg aaccaacaaa
//
LOCUS       DAORLS010000282         3884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12766_length_3884_cov_1.738835, whole genome shotgun sequence.
ACCESSION   DAORLS010000282 DAORLS010000000
VERSION     DAORLS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12766_length_3884_cov_1.738835"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..2082)
                     /gene="paaZ"
                     /locus_tag="PK977_16430"
     CDS             complement(10..2082)
                     /gene="paaZ"
                     /locus_tag="PK977_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HRF19764.1"
                     /translation="MNKLGNYITGKWITGDGEGQLLYNAVTGEPFAAATTKGLDFAAI
                     TDYARKVGNPALRKMTFHQRGNMLKALALHLRNHLPQFYEISYKTGATKADSWVDIEG
                     GIGNLFANASLRRRFPDDVIAIDGESHNLSKNNTFMGTHILSPKEGVAIHINAFNFPV
                     WGMLEKIAVNLLAGVPAIVKPATVTSYLTEVVVKEIIASGILPEGSLQLICGSAGDLL
                     DHVTSQDIVTFTGSASTGLMLKKTAAILENNVPFNMEADSLNCIVLGENVTPDKPEWD
                     IFIKEIRKEMTLKAGQRCTGIRRIFVPENKMEDVWKAIGTSLAQTVIGNPLNEKVRMG
                     SLAGETQRSEVRSQIQKLMASSQIVYGSLDSVEVIDADPKKGAFMSPVLLMNDKPHSC
                     TEPHEVEAFGPVSTIMPYQNMEDAIALSKLGKGRLVTTVVTADYKEARQYVIGAATHH
                     GRILVLNNECAKENTGHGSPLPLLVHGGPGRAGGGEEMGGIRGVKHYLQRTAIQGSPT
                     TITEVTNVYQPNAKGKDPGKHPFQKYFEELQIGDQIVTEKRLITSEDIDKFADLSGDH
                     FYAHIKSTDFSGTMFEQQVAHGYLIMSIAAGLFVDSYEKNPVLLNYGIDELRFTKPVY
                     PGAEIHIRFTCKEKLPNDKRVVEKPEDFKRGDDIEKGIVKWLVEMIDDSNEVTGVATI
                     LTMVARKN"
     gene            2204..>2574
                     /locus_tag="PK977_16435"
     CDS             2204..>2574
                     /locus_tag="PK977_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19765.1"
                     /translation="MKKILSVIIIAATLFVTKSANAQEKAKWAEKDAFHKVMSETFHP
                     AEEGKLEPIRKRSQEMMDVAIAWKNSTAPAGYDKEKIMKDLKALVKGTKKLNKNVQKN
                     ASDNDLKEELTELHDLFHRIT"
     assembly_gap    2575..2596
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2630..3103
                     /locus_tag="PK977_16440"
     CDS             2630..3103
                     /locus_tag="PK977_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HRF19766.1"
                     /translation="MKKILTVLIVAGSVTIISCGGGDKNHDHSGHKNDEPKTATDSLY
                     SEVMAGHDEVMPKMGKIRGAQKEAQRLIDSIAALPAKAQAEAASLKTKLEELVKDLSY
                     ADFAMDKWMTEFNMDSAKDNMEQRLKYLAEEKLKVNKVKDAILNSLAKADSLLKR"
     gene            complement(3185..>3636)
                     /locus_tag="PK977_16445"
     CDS             complement(3185..>3636)
                     /locus_tag="PK977_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19767.1"
                     /translation="TRFVSCLQQIQPTATPRWGKMNLQQMVEHFSADALRCASGRLKI
                     DTILTPAEDLDRMREFMMSEKPFKENTKNPLMSEEPPLTRYATLQAAIGELQEELIAF
                     FEAFEKNPHLVTRNPFFGDLNFEQNVQLLYKHALHHLRQFGAEPLSR"
     assembly_gap    3637..3669
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1125 a          873 c          747 g         1084 t
ORIGIN      
        1 cctgctttat taatttttac gggccaccat ggtcaatatg gttgccactc ccgtcacctc
       61 attggaatcg tcaatcattt ccacaagcca ttttacaata cctttttcaa tatcatcccc
      121 ccgcttaaaa tcttccggtt tttctactac tcttttatca ttcggcagtt tttccttaca
      181 ggtaaagcga atatgaattt ctgcacccgg gtaaaccggt ttagtaaacc gcaactcatc
      241 tattccgtaa ttcagcaaaa ccgggttctt ttcataactg tcaacaaaaa ggccggccgc
      301 aatgctcata atcaggtagc cgtgcgccac ctgttgttca aacattgttc cgctaaaatc
      361 agtggatttg atatgggcat agaaatgatc cccgctcagg tcagcaaatt tgtcaatgtc
      421 ttctgaagta atcaaccttt tttctgtaac gatctggtcg ccaatctgca gttcttcaaa
      481 atatttttgg aaaggatgtt tacccggatc ttttcctttc gcattcggct ggtacacatt
      541 ggttacttct gttatggtgg taggtgagcc ttgtatagcc gtacgctgca ggtaatgctt
      601 cacacccctt attccaccca tttcttctcc gccaccggca cggccgggcc cgccgtgtac
      661 taataacggc aaaggagatc catgtcctgt attctctttg gcacattcat tattcagcac
      721 caggatacgt ccatgatggg tagcggcacc gatcacatat tgccttgctt ctttataatc
      781 ggctgttaca actgttgtaa ccagtctgcc ctttcctaat ttgcttaatg caattgcatc
      841 ctccatgttt tggtaaggca taatcgtact tacaggtccg aatgcttcta cctcatgagg
      901 ttcagtgcag gaatggggtt tgtcattcat caataatacc ggagacataa aagccccttt
      961 ctttggatcg gcatctatca cttccacact atccagcgaa ccatatacta tttgcgatga
     1021 agccattaat ttttgaatct gacttctgac ctctgatcgt tgcgtttctc cggcaaggct
     1081 gcccattcta actttctcat tcagcggatt accgataacg gtttgggcta atgaagtgcc
     1141 tatcgctttc catacatctt ccattttatt ctcgggtaca aaaattctgc ggatgccggt
     1201 acatcgctga ccggctttta atgtcatttc ttttcgtatc tcttttataa aaatatccca
     1261 ctccggttta tcaggtgtta cgttttcgcc cagtactata cagttcaacg aatcagcttc
     1321 catattaaaa ggaacattat tctccaggat agccgccgtc tttttcagca tcagtcccgt
     1381 tgaagcgctg ccggtaaaag tgacgatatc ctgcgaagtg acatgatcca gcagatcacc
     1441 ggcactgcca cagatgagtt gtaatgaacc ttccggtaaa atgcccgaag caatgatctc
     1501 ttttactaca acctctgtca ggtaagaagt tacagtagcc ggctttacaa tagcaggtac
     1561 accggccagc aggttcacag caattttttc cagcataccc caaaccggga aattaaatgc
     1621 attgatatga atagccactc cttcttttgg agaaagaata tgtgtaccca tgaacgtatt
     1681 gttcttactc aggttatgac tttcaccgtc aatagcaatc acatcatcag gaaatctccg
     1741 acggagtgaa gcattggcaa ataagttacc gataccaccc tctatatcta cccagctatc
     1801 cgctttagta gctccagttt tataactgat ttcataaaat tggggaagat ggtttcttaa
     1861 atgcagggct aatgccttta gcatgtttcc ccgctggtga aaagtcattt tccgaagtgc
     1921 cgggtttccc actttacggg catagtcagt aattgcagca aaatcaagcc ctttagtagt
     1981 ggcggcagca aaaggttctc ccgtaacggc attataaagt agttgtcctt caccatcccc
     2041 tgtaatccat tttccggtta tatagtttcc cagtttattc atatagcatg gctgatttag
     2101 gcaacgaaaa tacccgaatt tgtaaaaact actttcaact gattttagtc ataacagcct
     2161 catatagata caaatacctt tgcacttcaa aaaacttgtc attatgaaga agatattatc
     2221 agtcattatt attgcggcaa ccttgtttgt aaccaaatct gccaatgcac aagaaaaggc
     2281 caaatgggca gaaaaagatg cttttcacaa agtgatgagt gaaaccttcc atccggctga
     2341 agaaggaaaa ctggagccta ttcgtaaacg cagccaggaa atgatggatg tagctattgc
     2401 atggaaaaat tcaaccgccc ctgccggtta cgataaggaa aaaattatga aggacctgaa
     2461 agcattggtt aagggaacaa aaaaactgaa taaaaatgtg caaaaaaatg cttcagataa
     2521 tgacttgaaa gaagaattga cagaacttca cgacctgttt caccggataa cagannnnnn
     2581 nnnnnnnnnn nnnnnnggtg aagatcatca ctaaaattta ataacaggta tgaagaagat
     2641 tttaaccgtt ttaattgttg ccggcagcgt aactatcatt tcctgcggag gcggtgacaa
     2701 aaaccatgat cacagcgggc ataagaatga tgaacccaaa actgcaacgg acagtttgta
     2761 cagtgaagta atggcaggcc acgatgaggt aatgccaaaa atgggcaaga tcaggggtgc
     2821 acaaaaagaa gcacagcggc tgatcgattc catagctgca cttcctgcaa aagcacaagc
     2881 cgaagcagct tccttaaaaa ccaaactgga agaactggta aaagacctca gctatgctga
     2941 ctttgctatg gataaatgga tgactgaatt caatatggat tctgccaaag acaatatgga
     3001 acagcgcctg aaatacctgg ctgaagaaaa actaaaagtg aataaagtaa aagatgctat
     3061 tcttaacagc ctggcaaaag ccgattcatt attaaaacga taagaagata attatcctga
     3121 tactaagact cttcctgagg aagcccatga gcgaagtccg ggaagagttt tttattccgg
     3181 tacactaacg gctcaaaggc tctgcaccaa actggcgaag atgatgcaat gcatgtttat
     3241 aaagcaactg cacattctgt tcaaaattca aatcaccaaa aaaaggattt cgggttacca
     3301 ggtgaggatt cttttcaaac gcctcaaaaa atgcgatcag ttcttcctgt aattcgccaa
     3361 tagctgcctg taatgtagca tagcgggtaa gcggaggctc ttcagacatc aacgggtttt
     3421 tggtattctc tttaaacggc ttctcactca tcataaactc tctcatccgg tcaagatctt
     3481 ctgcaggggt aagtattgtg tctatcttca atctgccgct ggcacaacga agggcatccg
     3541 ctgaaaaatg ctctaccatc tgctgtaaat tcattttccc ccaacggggt gtcgcagtag
     3601 gctggatttg ctgcaaacag gaaacaaacc tggtgcnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnna aaaaggttcg aaggagcaaa ttacaaaaac aggtgctcat gaaaaagccc
     3721 ttcatcagcg aagggcttaa aaagaggtta ataatattaa ttattccaat ttcttatcga
     3781 tcgctttgca tacagcattg aaaatttcct ctacagtacc ctctcctttt acagattcaa
     3841 atttcttcac ttttttataa tgctccgaca ccggagctgt ttct
//
LOCUS       DAORLS010000283         3870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12824_length_3870_cov_2.167497, whole genome shotgun sequence.
ACCESSION   DAORLS010000283 DAORLS010000000
VERSION     DAORLS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_3870_cov_2.167497"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1252)
                     /locus_tag="PK977_16450"
     CDS             complement(35..1252)
                     /locus_tag="PK977_16450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF19768.1"
                     /translation="MRFTEFNFRPELLEGIEASNYETATPVQEQVIPSILDGRDIIAS
                     AQTGTGKTAAFLLPLMNTLLNKHVDGQVGALIIVPTRELAIQISQHIEGLSYFTHLSS
                     IAIYGGSDAQNFVAEKKALQMGADIIVCTPGRMIAHLNMGYVNFKGLQFLVLDEADRM
                     LDMGFQDDINRIIKALPEKRQNLLFSATMPEKIRQLARKILHNPVEVNIAISRPPEKV
                     VQKAFVVYEPQKLPLIKKLLKETPYKNALIFCSKKQTVKQLRRELKQGGFNIEEIHSD
                     LEQSQRESVLQAFTSGRIPILVATDILSRGIDIDTIDLVINYDVPHDGEDYIHRIGRT
                     ARAEADGVAYTIVSEKEQNRFASIEKLIDKEVEKGLIPHELGPAPAYSPRQRSRGNRP
                     KQRFYRSNNNRKK"
     gene            1372..2013
                     /locus_tag="PK977_16455"
     CDS             1372..2013
                     /locus_tag="PK977_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19769.1"
                     /translation="MRIFTFSLFLVLFTAASFVSGPGTTPVSPLAIYSAEWNNPVYLK
                     CNTAKDANYMTGPEKEVIYILNLLRTNPALFAKTVVKQYPEKSGQGYLVNSWYYTSLL
                     DTLKKIKPIVVLKPDINCFNSAQCHALYAGATGYAGHDRSSPECQSKKYFNGECCDYG
                     NKEPLEIVMSLLIDEGVPSLGHRDVCLSPYKKIGVSIQAHQKYRYNAVLDFSY"
     gene            complement(2010..>2371)
                     /locus_tag="PK977_16460"
     CDS             complement(2010..>2371)
                     /locus_tag="PK977_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19770.1"
                     /translation="PAKVRSNFKRDYPNAINVRWTKCRGDWTATFANGIFLSTAVYHA
                     NGDRRDTRTPITRNEIPRITLDSVFKKRPGINIGDVIKIDLPGKLDEIFRIKTGTDAG
                     SEFLFFNKLGEMIKYDY"
     assembly_gap    2372..2399
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2400..2632)
                     /locus_tag="PK977_16465"
     CDS             complement(<2400..2632)
                     /locus_tag="PK977_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19771.1"
                     /translation="MKKVLIMAGSFAICFSLLAQGKSGEKGKDKQKNKNNAAQVDDKG
                     KNKDKEKANDDKAKNDEHDRKIWDGVAGTSDCG"
     gene            complement(2670..>3870)
                     /locus_tag="PK977_16470"
     CDS             complement(2670..>3870)
                     /locus_tag="PK977_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF19772.1"
                     /translation="NNGLGLKLLGLSGDEVLPAAIYEQCKQAALQQVRGTVQADILKE
                     DQAQNTCIFSTEFALKLMGDVQEYFIQNKVRNFYSVSISGYHIAEAGANPITQLAFTL
                     ANGFTYVEYYLSRGMNIDEFAPNLSFFFSNGMDPEYSVIGRVARRIWAKAMKYKYKAN
                     KRSQMLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTE
                     ESVRRAMAIQLIINRELGSAKTENFIQGSFAVEELTDLVEEAVLAEFDRITERGGVLG
                     AMERMYQRNKIQEESLYYEHQKHTGELPLIGVNTFLNKKGSPTIIPGEVIRSTIEEKE
                     QQIENLKAFWTRNEQKSGEMLIRLKSVAINNGNLFAELMETVKYCSLGQITHALYEVG
                     GQYRRNM"
BASE COUNT         1017 a          891 c          721 g         1213 t
ORIGIN      
        1 agtacagcaa tttgcattct taaaaataac ctttttattt tttccggttg ttattagaac
       61 gataaaatcg ctgttttggt ctgttacccc tgcttctctg ccggggagaa taggcaggag
      121 caggacctaa ttcatgggga atcaatcctt tttccacctc tttatctatc aatttttcaa
      181 tagaggcaaa ccggttctgt tctttttcgc tgactattgt gtaagccaca ccatcggcct
      241 cagccctggc agtacggcca atacggtgta tataatcttc accgtcatgc ggtacatcat
      301 agttaatcac caggtcaatg gtatctatat cgatccccct gcttaaaata tcagtagcca
      361 ccaatatagg aatacgtccg ctggtaaaag cctgtaaaac actttcccgt tgtgattgct
      421 caaggtcact atgtatttct tcaatattga atccgccctg ttttaattct ctgcgtagtt
      481 gttttactgt ttgtttttta ctgcaaaaaa tcaatgcatt tttataaggt gtttccttga
      541 gtagtttttt gatcaggggt aatttttgcg gctcgtatac aacaaaagct ttctgtacca
      601 ctttttctgg cggccttgaa atagcaatat ttacttcaac cgggttatga agtattttcc
      661 tggccagctg tcttattttt tccggcattg ttgccgagaa taaaaggttt tgcctctttt
      721 cgggtaatgc tttaatgata cggttgatat catcctggaa gcccatatca agcatccggt
      781 ccgcttcgtc caaaacaaga aattgcagcc ccttgaaatt tacataaccc atattgaggt
      841 gggcgatcat tctgccgggt gtacatacaa tgatgtcagc acccatttgc aatgcctttt
      901 tctccgcaac aaaattttgt gcatcgctac caccatatat agcaatagaa ctcagatgag
      961 tgaaataaga cagtccctca atatgttgtg agatctgaat ggccagttct ctagttggta
     1021 caataatgag agcccccacc tgtccgtcta catgtttatt taggagggta ttcatcaggg
     1081 gcaacaggaa agctgctgtt ttacctgtac cggtttgtgc agacgcaatg atatcccggc
     1141 catccaaaat agaaggtatc acttgttcct gtacaggggt ggctgtttcg tagttagagg
     1201 cctcaatacc ttctaacaac tcagggcgga agttaaactc tgtaaaacgc aatagttgaa
     1261 ttttatgaac gctgcgaagg tagatgaaaa tacgagggcg tctttcacat ttttttaagc
     1321 ccggcactgc aataaataat cgatgctgcc gtcaataaac aattactggc tatgagaata
     1381 tttacattta gtttgtttct ggtacttttt actgctgcct cctttgtcag cgggccagga
     1441 accacacccg tttcacctct cgccatttat tctgctgaat ggaacaatcc ggtatacttg
     1501 aaatgcaata ctgcaaaaga tgccaactat atgacaggtc cggaaaaaga agtaatctat
     1561 attttgaacc tgctgcggac caatcctgca ttgtttgcca aaacagtagt caaacaatat
     1621 cctgaaaagt ccggccaggg atacctcgtc aactcctggt actataccag cctgctggat
     1681 accttgaaga aaattaaacc cattgtcgtg ttaaaaccgg atataaattg ttttaatagt
     1741 gcccaatgtc atgctttgta tgcgggagct actggttatg cggggcatga ccggagcagc
     1801 cctgagtgtc agtcgaaaaa atatttcaat ggtgaatgtt gtgattacgg caataaagaa
     1861 ccgctggaaa ttgtaatgtc tctgttaata gacgaaggtg ttccttcgtt ggggcacaga
     1921 gatgtttgtc tcagccccta caaaaaaatt ggtgtgtcta tacaggcaca ccaaaaatat
     1981 cgctacaacg cagtgctcga cttcagctat taatagtcat atttgatcat ctcaccgagt
     2041 ttattaaaaa ataaaaattc agaacctgca tcagttcccg ttttgatccg gaaaatttca
     2101 tccagttttc cgggcagatc aatcttgata acatccccaa tatttatccc gggtcttttc
     2161 ttaaatacag aatcaagcgt aattcggggt atctcatttc tcgttatagg tgtacgggta
     2221 tctctcctgt caccgttggc atgataaacg gctgttgaca gaaaaatgcc attagcaaag
     2281 gttgcagtcc agtcaccacg gcattttgtc caccttacgt ttattgcatt gggataatct
     2341 ctcttaaaat ttgaccgtac tttggcaggc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2401 ttccgcaatc actggttccg gctacaccat cccaaatttt tcggtcgtgt tcatcattct
     2461 ttgccttatc gtcatttgcc ttttctttat ccttattctt tcccttatcg tcaacctgag
     2521 cagcattatt cttgttcttt tgcttatcct ttcctttctc accactcttc ccttgtgcta
     2581 ataaagaaaa acaaattgca aaactgcctg ccattatcag aacctttttc ataactataa
     2641 attttattca agtaaaaact gattctttgt tacatattac gccggtactg tccgcctact
     2701 tcatataagg catgtgtaat ctggccaagg gaacaatact taactgtttc cattagttcc
     2761 gcaaacaagt taccgttatt aattgcaact gacttcaatc gtattagcat ctccccagac
     2821 ttctgctcat tcctggtcca gaaggctttc aggttctcaa tctgttgctc tttttcttct
     2881 atggtgctac gaatcacctc tcccggaata atcgtagggc ttcccttttt gttgagaaat
     2941 gtatttacac caattaatgg caactctcct gtgtgtttct gatgttcata atacaggctt
     3001 tcctcctgaa tcttattccg ctggtacata cgttccattg ccccaagcac accgcctctt
     3061 tctgttatac ggtcaaactc ggctaacact gcttcttcta ccaggtcggt caattcttcc
     3121 accgcaaaag atccctgaat aaagttctct gtcttggctg aacccagttc acggtttata
     3181 atcaattgaa tcgccattgc cctcctaaca ctttcttcag taggtgtggt gatcgcttca
     3241 tcataggcat ttgtatgcaa tgaattacag ttgtcataga tcgcatacag tgcctgcagc
     3301 gtagtacgga tatcattgaa atcaatttcc tgtgcatgta aacttctgcc ggatgtctga
     3361 atatggtatt tcagcatctg gctgcgtttg ttcgccttat acttatactt catggctttt
     3421 gcccagatac gccttgccac acggccaatt acactgtact cgggatccat tccattactg
     3481 aagaagaaag aaagattggg ggcaaactca tcaatattca tccccctgct caggtaatac
     3541 tctacatagg taaacccgtt ggccagcgta aaagctaact gggtaatagg gtttgctcct
     3601 gcttctgcaa tatggtagcc acttatggaa acagaataaa aattacgcac cttgttctga
     3661 ataaaatact cctgcacatc ccccattaat ttcaaagcaa actcggtaga gaaaatacag
     3721 gtgttctgcg cctggtcctc tttcaatata tcagcctgaa ccgttcccct tacctgctgc
     3781 aaagctgcct gcttgcattg ttcgtaaata gcagcgggca aaacctcatc accgctaagg
     3841 ccaagcagct ttaaaccaag tccgttattc
//
LOCUS       DAORLS010000284         3868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12835_length_3868_cov_1.662733, whole genome shotgun sequence.
ACCESSION   DAORLS010000284 DAORLS010000000
VERSION     DAORLS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12835_length_3868_cov_1.662733"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>976
                     /locus_tag="PK977_16475"
     CDS             <1..>976
                     /locus_tag="PK977_16475"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRF19773.1"
                     /translation="WQVEIYTVKATDYWVARIDAGTGQLLDKNNLVVSCDFGVADNKK
                     CRHGFLPHKRPEDKPVFQVPFFMKNAGGKPAENNKKFSPFYNDGSSYQVYAYPVESPN
                     HTAPLPPADARTTVTNPSVTAASPFGWHDTNGAAGAEFTTTQGNNVHAYTDLNADNNP
                     DAGSSPDGTASLTFVFPIDLTQPPSAYRPAAVTNLFYWNNFIHDFMYGYGFDEANGNF
                     QVNNYGNGGLGNDDVRAEAQDGSGTNNANFFTPADGQRPRMQMYVGTNPTPDVDGDLD
                     NLVIVHEYAHGISNRLTGGPANVSCLSNQEQMGEGWSDWYGLMMTMKPT"
     assembly_gap    977..996
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <997..>2011
                     /locus_tag="PK977_16480"
     CDS             <997..>2011
                     /locus_tag="PK977_16480"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRF19774.1"
                     /translation="RGVGTYLFGEPANGPGIRPNRYSTDMLINPTTYDDIKTLAVPHG
                     VGYVWASMIWDLTWKLIDKHGMTNGFNIAMNIVTEGMKLQPCSPGFVDGRNAIIAADQ
                     SLYAGANRCLIWEVFARRGLGFSASQGLSSSRSDGTEAFDMPSNCFVDAVPTTISVCA
                     PANAVATINVGDGYSGPVTLSATGNPAGTTVSFSPNPVTGPASSTMTIGNTGAAAPGT
                     YTVTVSGTDGVLTYNDNFSLTIQAAAPAATTLVSPANGAVNVVNTVLNWNVLAAAETY
                     EVQVATDAGFTNIVASATGLTSPTYTPAGLVNLTTYFWRTRAVNICGTGVYSSVFSFT
                     TGNV"
     assembly_gap    2012..2061
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2062..>2404
                     /locus_tag="PK977_16485"
     CDS             <2062..>2404
                     /locus_tag="PK977_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRF19775.1"
                     /translation="SAGTPTVTSTLSIAACGTITGVKVQNLNITHAFVGDLRVTLTSP
                     LGTVVTLFDRPGVPASTFGCSGDNLAIGFDDAAILTATDFENACGNLPAISGIYQPVN
                     LLSAFSGENMAG"
     assembly_gap    2405..2496
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2497..>3868
                     /locus_tag="PK977_16490"
     CDS             <2497..>3868
                     /locus_tag="PK977_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRF19776.1"
                     /translation="PCAGAPTITTSGSLTPFTACSGNASAEQSFTVSGTDLTANLVVT
                     APAGFEVSTTSGSGFGSSVSLAPSSGTVPSTTIYVRMAATATGTPAGNIACSSTGATT
                     QNVAVSGTVNTAPTVTCPANVTVSNDPGLCSAIVNYPAASVTGSPAPTVTYSTPSGSS
                     FPVGTTTVTVTATNICGIATCTFTVTVNDTENPTASPVSSNFQCGAEVPPPSAAVITD
                     EADNCGVPTVTFLPAATTNNGGTGCAANPLIITRYYRVQDAAGNFIDVPHTITIVDNT
                     PPVVTCPANITVNNTPGTCGAVVNFTPTATDNCGGAVTITSIPASGSTFPVGTTNVYV
                     TATDACGNSATCTFTVTVNDTQAPVITCPSNISVANTPNQCGAIVNYSLPTVTDNCPG
                     STLNAPIGTPNSFNGITFDITNNTTSPMTITGFTAPIGTGTHPVEVYYTTSATTAVGN
                     EQNAANW"
BASE COUNT         1012 a          836 c          853 g         1005 t
ORIGIN      
        1 ctggcaggtt gaaatttata ctgtcaaagc cactgattac tgggtggcaa gaattgatgc
       61 aggtaccggc cagttattag ataaaaacaa cctggttgtt tcttgtgatt ttggggtggc
      121 agacaataaa aaatgccgtc atggtttttt gccacataaa agaccagaag ataaacctgt
      181 attccaggtt cctttcttta tgaaaaatgc cggtggtaaa cccgctgaga ataataagaa
      241 gttcagtcct ttttacaacg atggaagcag ctaccaggta tatgcttacc cggtagaaag
      301 tcctaaccat acagcacctc ttcctcctgc tgatgcaaga actacggtaa ccaatccgtc
      361 tgtgacggca gcctccccat ttggctggca tgacaccaat ggtgctgccg gtgcagagtt
      421 tactactacg cagggtaata atgtacatgc atatacagat ttaaatgcag ataacaatcc
      481 tgatgctggt tccagccctg atggtaccgc tagcttgact tttgtgtttc ctatagatct
      541 tactcaaccc ccaagtgctt acaggcctgc agctgtaacc aatcttttct actggaataa
      601 ttttattcat gattttatgt atggctacgg ttttgatgaa gccaacggta acttccaggt
      661 gaataactat ggaaacggtg gtttgggaaa tgatgatgta agagccgagg cacaggatgg
      721 aagcggtact aacaatgcta atttcttcac accggcagat ggtcagcgtc ccaggatgca
      781 gatgtatgta ggtacaaacc ctactccgga tgtggatggt gacctggata acctggtaat
      841 tgtacatgag tatgcacacg gtatatccaa caggctgaca ggtggacctg ccaatgtaag
      901 ttgcttgagt aaccaggaac aaatgggtga gggttggagc gactggtatg gcctgatgat
      961 gactatgaag ccaacannnn nnnnnnnnnn nnnnnngaag aggcgttgga acttatttat
     1021 ttggtgagcc ggcaaacgga ccgggtatca ggcctaatcg ttattcaacc gatatgctga
     1081 ttaacccgac aacctacgat gatatcaaaa cactggctgt gccgcatggg gtgggttatg
     1141 tatgggcaag tatgatatgg gatcttacct ggaaattgat tgacaaacat gggatgacca
     1201 atggattcaa tattgcaatg aatattgtaa cagaaggtat gaaactgcaa ccctgcagcc
     1261 ccggctttgt agatggaaga aatgcaatta ttgctgcaga ccagtcatta tatgccggag
     1321 ccaacaggtg tcttatctgg gaagtttttg ccagaagggg attaggattt agtgcatcac
     1381 aggggttatc cagcagcagg agtgatggta cagaagcatt tgatatgcct tctaattgtt
     1441 ttgtagatgc tgttccaaca actatctctg tatgtgcacc agccaacgct gttgcaacca
     1501 ttaatgtagg cgatggatat agtggccctg ttacactgag tgcaaccggt aatcctgcag
     1561 gaacaactgt ttctttctcc cccaatccag ttacaggtcc tgcatccagt acaatgacga
     1621 ttggaaatac cggggcagcc gctcctggta cctatactgt tacagttagt ggtactgatg
     1681 gggtgcttac ttataatgat aatttctccc ttactataca agcagcagct cctgcagcta
     1741 caacattagt tagccctgct aacggagcag tgaatgttgt aaatactgtt ctaaactgga
     1801 atgtgctggc cgctgctgaa acgtatgaag tacaggtagc aaccgatgcc gggtttacta
     1861 atattgttgc cagtgccaca ggattgacca gcccaaccta tactccggcc ggcctggtta
     1921 acctgactac ttatttctgg agaacaaggg ccgttaatat ttgcggaaca ggagtgtatt
     1981 cttcagtatt ctcatttaca accggtaatg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn ncatctgctg gaacacccac cgttacttct acactttcaa
     2101 tagcagcttg tggtacaata acgggtgtga aagttcaaaa ccttaatatt acgcatgcct
     2161 ttgtaggtga tcttagggta acgcttactt cgccattggg aacagtggtc actctatttg
     2221 acaggcccgg agtacctgca tcaacatttg gatgcagtgg cgataatctg gctatagggt
     2281 ttgatgatgc agccatttta accgcaaccg attttgaaaa cgcatgtgga aaccttccgg
     2341 caatcagtgg tatttaccag ccagtcaacc ttctttcagc atttagtgga gaaaatatgg
     2401 cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacct tgtgcagggg ctccgacaat
     2521 tacaaccagc ggatcactta ctcctttcac cgcttgtagc ggtaatgcat ctgcagaaca
     2581 atctttcact gtatccggta cagatcttac agcgaacctg gtggtaacag ctcctgctgg
     2641 ttttgaggta tccactactt caggcagtgg tttcggcagc agtgtatcac tggcaccttc
     2701 ttctggtact gtgccctcta ctacaattta tgtaagaatg gccgctacag cgacaggaac
     2761 acctgcaggc aatattgctt gtagttcaac tggtgctaca acacagaatg tagcggttag
     2821 tggtactgtg aatacagctc caactgttac ttgcccggcc aatgtaactg taagtaatga
     2881 tccgggtctg tgtagtgcta tagttaatta tccggctgca tccgtaaccg gaagtccggc
     2941 gccaacagtt acttattcta cgccatccgg aagttctttc cccgtaggca ctactacggt
     3001 aacagtaaca gcaacgaaca tttgcggaat agctacctgt acatttactg ttacagtaaa
     3061 tgatacagag aatcctactg cgagcccggt atcttctaat ttccagtgcg gtgcagaagt
     3121 acctccacca agtgcagctg ttattaccga tgaagctgat aactgtggtg ttcccacagt
     3181 caccttcctt ccggctgcca ccaccaataa tggtggaaca ggctgtgctg ccaatccgtt
     3241 gatcattacc cgctattata gggtgcagga tgcagcaggc aactttattg atgtgcctca
     3301 tacaataact attgttgata atacgccacc tgtagttacc tgtcctgcta atattacagt
     3361 aaataataca ccgggtacct gcggagcggt ggtaaacttt acgccaacag ctacggataa
     3421 ctgcggtggt gctgtaacca tcacgtcaat tccggcatcg ggaagtacat tccctgtagg
     3481 tactaccaat gtgtatgtta ctgctacaga tgcttgtggt aacagtgcta cctgtacctt
     3541 cactgtaacg gtaaatgata cacaggcacc agtgatcact tgtccgtcca atatttcggt
     3601 agctaatacg ccaaaccaat gcggtgcaat tgtaaattac agtttaccaa cagtaacaga
     3661 caactgtcct ggctctacgt tgaatgcgcc aattggtaca cccaattcat ttaacggtat
     3721 tacttttgat attaccaata acacaacttc tccaatgacg ataacagggt ttactgcacc
     3781 aattggtaca ggaactcacc cagttgaggt gtattacacc acatcagcaa caacagcagt
     3841 tggcaatgaa caaaacgctg ccaattgg
//
LOCUS       DAORLS010000285         3867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12840_length_3867_cov_1.722718, whole genome shotgun sequence.
ACCESSION   DAORLS010000285 DAORLS010000000
VERSION     DAORLS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12840_length_3867_cov_1.722718"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..>731
                     /locus_tag="PK977_16495"
     CDS             333..>731
                     /locus_tag="PK977_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19777.1"
                     /translation="MTIRKQIIFSSGFWSLLIIFTFMACKSKDKAVNTNNDPQSIFFD
                     FQVTGSEEEDSVTVLLHFRNSDIEGEAFRLDKPSEVRLDGESLDYNSSKMTGHYYEKR
                     KSVGSFSGDHKIIFTDVKGNTYQENFSFRPF"
     assembly_gap    732..763
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <764..1051
                     /locus_tag="PK977_16500"
     CDS             <764..1051
                     /locus_tag="PK977_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19778.1"
                     /translation="HFGDSLALQFDGLEKEDYLLVLMTDTSFPGKGLNRVDTIRNGKL
                     IISPNELQQLTPGPVQIIFTREFERPLENGTKSGGRLAITYRLSREFILQE"
     assembly_gap    1263..1371
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1372..>1632
                     /locus_tag="PK977_16505"
     CDS             <1372..>1632
                     /locus_tag="PK977_16505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRF19779.1"
                     /translation="ESGAGMSLATERPAEKVLLIVLTSDRGLAGAFNANVIKLAKATI
                     AEKYTEQHKKGNVTIWNIGKKGYEHFAKLNFKTDATYKDIFLH"
     assembly_gap    1633..1662
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1663..2073
                     /locus_tag="PK977_16510"
     CDS             <1663..2073
                     /locus_tag="PK977_16510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HRF19780.1"
                     /translation="SAAAMKACEEKQFDVVELVYSQFKNAATQRFVVERFLPIPKVDK
                     KDGATKADFIYDPSKEQLIAELMPKILNTQLFKAVLDSNASEHGARMTAMDKASENAN
                     EMLRSLKISYNRARQATITTELTEIVSGAAALQG"
     gene            2192..>2759
                     /gene="scpB"
                     /locus_tag="PK977_16515"
     CDS             2192..>2759
                     /gene="scpB"
                     /locus_tag="PK977_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRF19781.1"
                     /translation="MEIANLIPHIESLIFASDKPVTTLELTDLVNNALGFMDDKVSLD
                     QVEAALEGIQEKYSSEFYPFEVIQSGGGWQFLTKKEYHKTVAQLNGDKFLKRLSAAAL
                     ETLAIVAYKQPVTKGEIESIRGVSSDYALQKLLEKELIIISGRNEKAPGQPLLYSTSK
                     NFMDYFGINSAEDLPKIKEVIAEQLVEPT"
     assembly_gap    2760..2798
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2979..>3867
                     /locus_tag="PK977_16520"
     CDS             2979..>3867
                     /locus_tag="PK977_16520"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRF19782.1"
                     /translation="MQLTREQLLSAAGEYGTPVYIYHAERINEQYLRLTNAFKGTDSR
                     FFYAAKALTNIHVLRYIREIGCNVDCSSINEARLALYAGFEPSNILYTSNNIAFEEIE
                     EAKHLGIHINIDSISNLEKFGKKFGHTYPVGIRLRPNIMAGGNLKISTGHAASKFGVP
                     LEDIEQVLRVQKETGLFIRTLHIHTGSEIKDVEVFAKGIEVLFEVAQHFPELEVIDLG
                     GGFKVPYMPGEEGTDIEKLGKKVKEEFEEYERKYNRHLQVWFEPGKFLVSEAGYFITK
                     VNVLKKAGNISFAGVDTGLN"
BASE COUNT         1170 a          661 c          832 g          994 t
ORIGIN      
        1 ttttcttcac atctttttct gacttactgc cgccgaataa tttagaaatg aaaccaagca
       61 tggtattggg aattgggtaa ttggttgatt agttaatatt atatgatcaa aagggaatag
      121 ctaaaacttt cactattaat ccctttattg tcaaaaacgg tgctacaaaa aagtacaggc
      181 catattgaca gccctcaaaa gaagcccttt tgaagggtgg caaagataag tcatagtttg
      241 gagttaatca gcaggtttaa acacggcggc tggctaatta aaggggtgag tttcaacata
      301 tatttccgtt gttgatgtaa ctttcgcccc tcatgactat caggaaacag atcatctttt
      361 catccgggtt ttggagcttg ctcatcattt tcacctttat ggcctgcaaa agcaaggata
      421 aagctgtaaa cacaaataat gatccccaat ccatcttttt cgatttccag gtaactggca
      481 gtgaagagga ggattcagtt actgttttgc tgcatttcag aaacagtgat atagaagggg
      541 aggcatttag gctggacaaa ccctctgaag taagactgga tggagaaagc cttgattata
      601 acagctcaaa aatgaccggg cattattatg aaaaaagaaa gtcagtgggt tcattttccg
      661 gggatcataa aattattttc accgatgtaa aaggtaacac ctaccaggag aatttctctt
      721 ttcgcccttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncattttg gtgatagcct
      781 tgcattgcaa tttgacggcc ttgaaaagga agactatttg ctggttttaa tgacagatac
      841 cagttttccc ggaaagggac ttaaccgggt cgatacgatc aggaatggga aattgatcat
      901 tagccccaac gaactgcagc aactaacccc cggacctgta caaataatat tcacacggga
      961 atttgaaaga cccctcgaaa atggcacgaa aagcggcgga agactggcca taacctatcg
     1021 cctgagcagg gaatttattc ttcaggagta gcttgaaaaa gaaggattta cacaaataaa
     1081 tcacagtgat cgtttggagg cttattattt tgattcatcg atggaatttt agctgtcagg
     1141 ccataccttt gcggccaatt caagaaaaac tatgtcaggt cagttaaaag aggtaagaaa
     1201 ccggattaaa tcggtacaaa gtacgcagca gattacaaaa gcaatgaaaa tggtgagtgc
     1261 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagagtggt
     1381 gccggtatgt cattagctac agaacgtccg gcagaaaaag tgttgctgat tgtacttacc
     1441 agtgaccgtg gccttgcagg tgcatttaat gccaatgtta ttaaattggc taaggctaca
     1501 attgctgaaa agtatactga acagcataaa aaaggaaatg taacgatctg gaatattggt
     1561 aaaaaaggat acgaacattt tgcaaagctc aattttaaaa ctgacgccac atataaagat
     1621 atttttcttc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntctgccgc cgctatgaaa
     1681 gcttgtgaag aaaagcagtt tgatgtagtg gaactggtat acagccagtt caaaaatgca
     1741 gcgacacagc gttttgttgt ggaaagattc cttccgattc caaaagtaga taagaaggat
     1801 ggtgcaacga aagcggattt catctatgat ccttccaaag aacaattgat tgcagaatta
     1861 atgcctaaaa tcctcaatac tcagttattt aaagcagttc ttgattccaa tgcgtctgaa
     1921 catggtgcca ggatgactgc catggataaa gccagcgaga atgccaatga aatgctgaga
     1981 agcctgaaaa tatcttacaa ccgggccaga caggccacca ttactaccga gttgaccgag
     2041 attgtaagcg gtgctgctgc attacaagga taaaaaagcc attttattaa atatattgaa
     2101 ctggaatgct cagttcaaaa tgttaataac tgtgtttctg acttcctcgg ggtaatatta
     2161 atttcgttca aatcgtttcg aaacatcaac catggaaata gctaatctga tacctcatat
     2221 tgaatccctg atttttgcca gtgataaacc ggttacaacg ttagagctca ctgacctggt
     2281 aaataatgca ctgggcttta tggacgataa agtttccctt gaccaggtag aggcggcgtt
     2341 ggaaggtata caagaaaaat acagcagcga gttttatcct tttgaagtga tacaaagtgg
     2401 cggtggctgg caatttttga ccaaaaagga atatcataaa acagtcgccc agttgaacgg
     2461 agataagttt ttaaagcgtc tttctgcagc tgcacttgaa acactggcta ttgtcgccta
     2521 taagcagccg gttaccaaag gtgaaataga gtccatccgt ggtgtaagtt ccgattacgc
     2581 attacaaaag ttacttgaaa aagaactgat catcattagc ggtaggaatg aaaaagcgcc
     2641 gggccagcct cttttatatt ccacttcaaa aaacttcatg gattatttcg gcattaattc
     2701 cgccgaggat ctgccaaaga tcaaggaagt aattgcagag caactggtag agccaacggn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gcagatgaac aacttgacaa
     2821 tgcagaggca ctagcggtaa atgatggtgg tgaactggtg gttaatgatg ccggcagtga
     2881 gggtgaagaa aattagaatt atttttttga aataatattt gccatgccat tccttcggga
     2941 gtggcatagt tgttttaact attttcgcaa tcatgacaat gcagcttacc agggaacaat
     3001 tactaagtgc ggcgggtgaa tacggaacac ctgtttatat atatcatgcc gaacggataa
     3061 atgaacaata tttgcggctt actaatgctt ttaagggtac agactcccgg tttttttatg
     3121 ctgcaaaagc attgactaat attcatgtgc tgcgatacat cagggaaatt ggctgcaatg
     3181 tggattgcag ctctattaat gaagcaaggc ttgcactata tgccgggttt gaaccctcaa
     3241 acattttgta cacatccaat aatattgctt ttgaagaaat agaggaagca aagcatttgg
     3301 gtatccatat caatattgac agtatttcaa acctggaaaa atttggtaaa aaattcggtc
     3361 atacataccc tgtaggaata cgactccggc ccaatattat ggcaggtggc aacctgaaaa
     3421 tatcaactgg tcatgcagcc agtaagttcg gagttccgct ggaggatatt gagcaggtgt
     3481 taagagtgca gaaagaaacc ggcttattta tccgcacttt acacatacac accggcagcg
     3541 agataaagga tgtggaagta tttgctaaag gtattgaggt gttatttgaa gttgcccagc
     3601 attttcctga actggaagtg attgacctcg gaggtggatt taaagtacca tatatgcctg
     3661 gggaggaggg gactgatatt gaaaaactcg ggaaaaaagt aaaggaagaa tttgaagagt
     3721 acgaaaggaa atataaccgg catctgcagg tgtggtttga acccggaaaa ttcctggtga
     3781 gtgaagcggg ttattttatc acgaaggtga atgtgctgaa aaaggccgga aacatatcat
     3841 ttgccggtgt ggataccggt cttaatc
//
LOCUS       DAORLS010000286         3860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12873_length_3860_cov_1.941919, whole genome shotgun sequence.
ACCESSION   DAORLS010000286 DAORLS010000000
VERSION     DAORLS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12873_length_3860_cov_1.941919"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>230)
                     /locus_tag="PK977_16525"
     CDS             complement(<1..>230)
                     /locus_tag="PK977_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19783.1"
                     /translation="TGIHPSMTVLQSLFNEGKAKVIQAVGYPTPNFSHFRATDIWMSA
                     SNSTQEVYSGWAGRYLNYEYPNFPNGYPNTDMP"
     assembly_gap    231..316
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<317..531)
                     /locus_tag="PK977_16530"
     CDS             complement(<317..531)
                     /locus_tag="PK977_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19784.1"
                     /translation="MKRRDFLLNTLPAAALLPEVINGYSVKAFNANSPLVQALMRGTT
                     LTDHVLVIVQLSGGNDGLNMVIPVADYS"
     gene            complement(553..2229)
                     /locus_tag="PK977_16535"
     CDS             complement(553..2229)
                     /locus_tag="PK977_16535"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRF19785.1"
                     /translation="MDRREFLTARRNKHAGQEEGGTAKPFRTNSGINTYTGPWSEQEV
                     VHLLKRTMFGAKKADVDYFKGLSMSQAVDQLLNPTAPNPAPPVKEYATSATPGVNADA
                     NILQGTTWVNDINSDGTVQSQRRASYKKWWTGVMINQDRSIREKLVMFWVDHFGNETT
                     DVGFGNWAYTQHSLIRQYALGNFKNLVRDISKDVMMLRYLNGYLNNKNAPDENYAREL
                     FELFTLGKGPGSQYTENDVKEAAKVLTGWQINGTTYTSVFNATRHDTTNKTFSSFFGN
                     TVITGRTGATAGDLELNDLINMTFNQAEVAKFIVRKLYRWFVYYAIDAGTEANVITPL
                     ADIFRSNNYEIKPVLSALFKSEHFFDMLNRGCYIKTPADQVIGSLREMNVQFRPETDW
                     DTNYGLWNTFFNWMVNMGQNLHDPPNVSGMPAFYQEPSFHEIWINSDSLPKRNQFTDT
                     MINTGYARNGFRVQFNCVTFAQTLPNPGNPNDLIDDALKIFFRNDLSAQSKQQIKTQI
                     LLTGQQWDYYWTNAWAAYVSNPTTANFNTVNTRLKSLFQYLFNLSEYQLA"
     gene            complement(2426..3604)
                     /locus_tag="PK977_16540"
     CDS             complement(2426..3604)
                     /locus_tag="PK977_16540"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRF19786.1"
                     /translation="MQEAYIVAGYRTAVGKAKRGGFRFTRPDDLAIEVIKGLMASVPQ
                     LEAKRVDDVIVGNAVPEAEQGLQFGRIISARALGIEVPGVTVNRYCASGLETIAMATA
                     KIRMGMADCIIAGGTESMSLVPTAGWKTVPAYSIAKDDPDYYLSMGLTAEAVAKDFNI
                     SREAQDEFSYHSHQKAIAAIQAGHFKPGILPITVEEVYLDQKGKKQKRNYVVDTDEGP
                     RADTSMEALAKLKPAFAAGGCVTAGNSSQTSDGAAFVIVMSEKMVNELGLKPIGRLVT
                     SAVAGVHPRIMGIGPVAAIPKALKQSGMNLSQIDLIELNEAFASQALAVIREAGLDPA
                     KVNINGGAIALGHPLGCTGCKLTIQNLNDMKRLGKKYGMITACVGGGQGIAGIIENIQ
                     "
BASE COUNT         1025 a          862 c          773 g         1114 t
ORIGIN      
        1 ggcatatctg tattaggata tccgttggga aaattgggat actcataatt caaataacga
       61 cccgcccatc cgctgtatac ttcctgtgta ctgtttgatg cactcatcca gatatcagtg
      121 gcacgaaaat gagagaagtt tggtgtagga taaccaactg cctgaattac tttagccttt
      181 ccttcattga agagagattg caacactgtc attgacggat gaatacctgt nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnngaat aatcagccac gggtatcacc atattcaatc catcattacc
      361 acctgaaagc tgtacgatga ccagcacatg atcggtaagg gtggtgccac gcatcaacgc
      421 ctgcaccagg ggtgagttgg cattgaaagc ttttacggaa tacccgttaa ttacttccgg
      481 caacaaggcc gctgcaggta aagtgttgag taaaaaatct cttcttttca ttgttgtcaa
      541 ttaaaattga gtttatgcca actggtactc cgataaattg aataaatatt gaaacaggga
      601 tttcagcctt gtatttacgg tattgaaatt ggctgttgta ggattagaca cataagcggc
      661 ccatgcattg gtccagtaat aatcccattg ctggccggtc aataatattt gtgttttgat
      721 ctgttgtttt gactgagccg acaggtcatt gcggaaaaat attttcagtg catcatcgat
      781 caggtcattg ggattacctg gattgggtaa ggtctgggca aatgtcacac agttgaactg
      841 gaccctgaaa ccattccggg catacccggt gttgatcatg gtatccgtaa actggttgcg
      901 tttcggtaat gaatccgagt tgatccagat ttcatggaaa gaaggctcct ggtaaaaagc
      961 aggcattcct gatacgttgg gcgggtcatg caggttctgg cccatattta ccatccagtt
     1021 gaagaatgta ttccataagc cataattggt atcccagtct gtctctggtc tgaactgtac
     1081 attcatttcc cttagtgaac ctatcacctg gtctgctggt gttttgatat aacaacccct
     1141 gttgagcata tcaaagaaat gctcactctt gaaaagtgca gacaagacgg gtttgatctc
     1201 gtaattattt gagcggaata tgtcagccag tggagtaata acattggctt ctgtgcctgc
     1261 atcaatggca taataaacaa accagcgata aagcttccga actatgaatt tagcaacctc
     1321 cgcctgatta aaagtcatat tgatcaggtc gtttagttca agatccccgg cggtagctcc
     1381 tgtgcgaccg gtaataacgg tgttgccgaa aaaagaagaa aatgtcttgt tagtggtatc
     1441 atgccgggtt gcattgaata cagatgtata agtagtgcca tttatttgcc agcccgttaa
     1501 tactttagca gcctctttta catcattctc tgtatactga gatccgggcc ctttacccaa
     1561 tgtaaataat tcaaataatt cccgggcata gttttcatct ggtgcatttt tattgttgag
     1621 ataaccgttc aggtagcgga gcatcatcac atctttggaa atatccctga ccaggttctt
     1681 gaaatttcca agtgcatatt gtcttatcaa tgaatgttgc gtataagccc agtttccaaa
     1741 tccaacatcc gttgtttcgt ttccaaaatg gtcaacccag aacatgacca gtttttcccg
     1801 aatacttctg tcctggttaa tcattactcc tgtccaccat tttttatatg aagccctcct
     1861 ttgtgattgt acagtaccat cgctgttaat atcgtttacc caggtagtac cctgcagaat
     1921 attagcatct gcatttacac cgggagtggc cgaggtagca tattctttta ccggcggtgc
     1981 aggattggga gccgttgggt tcagcagttg atcaacagcc tggctcatac taagtccctt
     2041 gaaataatct acatcagcct tctttgctcc aaacatggtc ctttttaaaa gatgtaccac
     2101 ttcctgttcc gaccaggggc ctgtatatgt attaattccg gaattagttc ggaagggttt
     2161 tgcagttccc ccttcctcct gtcctgcatg tttattgcgg cgggcagtca aaaattctct
     2221 tctgtccata cgtatggttt tggttcagca accgggttcg atataaatat tgctgaaaag
     2281 tttaagtaac gggtaacaaa aaatgagcct tacttcgctt attcgtaaaa atacagcctg
     2341 cctttgtgga aaaaacattt gctacttcct gtcagtaggc atcaagatac aaaagatgaa
     2401 gagaaaagaa aacccggaaa ccgaattatt gaatgttttc aatgatacct gcaataccct
     2461 gcccaccgcc aacgcaggca gtaatcattc catatttctt gccaaggcgt ttcatatcat
     2521 tcagattctg gatggtgagt ttgcaaccgg tacaacccaa tggatgaccc aatgcaatgg
     2581 cgccaccatt aatattcact tttgcaggat ccaaaccggc ttcccgaatg acagccaaag
     2641 cctgggaagc aaaagcttcg ttgagttcga tcaagtctat ttgggaaaga ttcatccctg
     2701 attgcttcag ggcttttgga atggcagcta ccggaccaat acccatgata cggggatgca
     2761 ctcctgccac agcagaagtt acgaggcggc caataggttt taatcccagt tcgtttacca
     2821 tcttctcact catcacaatc acaaaggcag ctccatcaga ggtttgagat gaattgcctg
     2881 ctgttacaca accaccggca gcaaaggcag gttttaattt ggccagtgct tccattgaag
     2941 tgtctgctct tggtccttca tccgtatcta ccacataatt tcgtttttgc ttcttgcctt
     3001 tttgatcgag ataaacttcc tccaccgtta tgggcagaat gcccggttta aaatgccccg
     3061 cttgtatagc tgcaatagct ttctgatgtg agtgataaga aaattcatcc tgtgcctccc
     3121 ggctaatatt gaaatcctta gccactgcct ctgccgttaa tcccatgctt aagtaataat
     3181 caggatcatc tttggcaatc gaataagcag gtacagtttt ccaaccagct gttggcacca
     3241 ggctcatact ttctgtacca cctgcaatga tacaatctgc catacccatg cggatttttg
     3301 cagtggccat agcgatcgtt tccaaaccag aggcacaata ccggttaact gtaacacccg
     3361 gcacttcaat acccaatgcc ctggcagaaa tgatgcggcc aaactgcaaa ccttgttccg
     3421 cttcgggaac agcgttaccc acaatcacat catctactct tttggcttcc agttggggaa
     3481 cagaagccat caatcctttt attacttcta ttgccaaatc atcagggcgg gtaaatcgaa
     3541 aaccacctct tttagcttta ccaactgctg ttctgtaacc agctacgata tatgcttcct
     3601 gcatgagaat gaattttcag tttcaaaatt aaaagttgtt tatgcaactt tctataccaa
     3661 aaatagggag acttatataa cctgccaaat accggcttaa aacttcagtt atcttcgcag
     3721 ctgttatgta taagttactg cgtactattc ttttctgttt ccctcccgaa ccggtgcacc
     3781 atttttccat gaatggactc aggttacttt gcagcctcgg tttcaaaaaa atactggcaa
     3841 aaatgttcag gccaaaggag
//
LOCUS       DAORLS010000287         3846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_12939_length_3846_cov_1.867845, whole genome shotgun sequence.
ACCESSION   DAORLS010000287 DAORLS010000000
VERSION     DAORLS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12939_length_3846_cov_1.867845"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK977_16545"
     CDS             complement(<1..390)
                     /locus_tag="PK977_16545"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HRF19787.1"
                     /translation="MNLIERAKNILMTPKTEWDKIDNETPNVQSILTTYVIPLVAIGA
                     IASFIGWGFIGKSYSYGFGSVKVSGVSLGIKYAIIALVGGIAGCYLLAVVIDALAPTF
                     KSEKNFGKSFQLAAYSSTAGWVGAVFNI"
     gene            complement(903..1334)
                     /locus_tag="PK977_16550"
     CDS             complement(903..1334)
                     /locus_tag="PK977_16550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF19788.1"
                     /translation="MKLHPYYRHLAVACMFTFASGSVLVQQASASGRPVKKISITAPA
                     EEEPAKKNKTKAKSSFASLNNSAVKIYPDAVKRDMHVVAKENDGKEIDFFVFDLQGTL
                     VQNYKMKAKDHYRLTGLSRGTYVYRVFSGDEETATGKFEIR"
     gene            complement(1366..1683)
                     /locus_tag="PK977_16555"
     CDS             complement(1366..1683)
                     /locus_tag="PK977_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19789.1"
                     /translation="MYVGFEILHRKTFTDYIDDVSTTYIDPALFNNYLAPEQIPVARQ
                     LMFRENIYNPSVSRVYTNEQRGDPKENDAFFSGILRFGWRLNGVNTPNGRAKRQLKCP
                     VYY"
     gene            complement(1703..2341)
                     /locus_tag="PK977_16560"
     CDS             complement(1703..2341)
                     /locus_tag="PK977_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19790.1"
                     /translation="MRTFVHTVRLSVLVSTFLSISLLSTAQSISSPNGKIEVGIGVGP
                     MIFLGDLGGSEGVGKTFIKDVDLPMTKISKGIYLNIYPMEWLGFRLAGNLGHVQGDDA
                     QAPDKGGAERFRRKRNLKFESKITEAYAALELYPTVFLEQYDGLAGKIRPYGLGGVGV
                     FHFNPKGEFIESNGNKRMVELKPLRLEGQGMAEYPDRKEYSLTQLEIVVGGG"
     gene            complement(2488..2928)
                     /locus_tag="PK977_16565"
     CDS             complement(2488..2928)
                     /locus_tag="PK977_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF19791.1"
                     /translation="MEWILKKFEELTPHELYTIMQLRNEVFVVEQNCVYQDADNKDRY
                     SFHLMGWKEDKLIAYTRLIPAGTAYAEPSIGRVVTSPSARGTGAGRELMEYSIDTIYK
                     LFGKQPIRIGAQLYLKKFYSSIGFEPTGDIYLEDGIEHIEMVKP"
     assembly_gap    2938..3005
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3006..>3846
                     /locus_tag="PK977_16570"
     CDS             3006..>3846
                     /locus_tag="PK977_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRF19792.1"
                     /translation="MQVKDLSTVHQLAGKLFRWPSSRKEWESYKLSEEQVAFFRENGY
                     LAGVKMLDEDQIEEIRKELAEIADPAHPGHALFYEFHSNESTDPSTILFHALGAWRIT
                     PGLHDVLWNPRFLVAASQLLGNVPVRFWHDQLFWKPAKQGGVVAWHQDYSYWTRTKPI
                     AHLTCWCGLDDATKENGCLQYIPGSHQWGLLPKPVIAGELEGIKDYLNEDQRQQFEKP
                     QLAEVKAGEAVFHHSLTLHGSGANTSPRPRRAFVINVFADGVRSDADEPMLDGVPSVP
                     RGEK"
BASE COUNT         1040 a          792 c          831 g         1115 t
ORIGIN      
        1 gatattaaat acagcgccta cccagcctgc agtagaggaa taagcagcta actggaatga
       61 tttgccgaaa ttcttttcag acttaaaagt gggagctagt gcatcaatta ctacagccag
      121 cagatagcaa cctgcaatac cgcctaccag ggcaataatg gcgtatttaa tacctaatga
      181 aactccggac acctttacac tgccaaaccc atagctgtag cttttaccga taaatcccca
      241 tcctatgaag gatgcaatag caccgatagc aaccagcgga attacataag tagtaagaat
      301 gctctgtacg ttaggcgttt cattatcaat tttgtcccat tctgttttag gcgtcatgag
      361 aatgttcttt gccctttcaa ttagattcat agcttggttg attttggttg tgcctactcg
      421 tttggctttt cggcattcgc ccccggccat aaaagatcgg gtcttctggt tttgatgaac
      481 ctaatataag acagtttttt aaaaacaggc cggcaatggg tctttttttt taaacctgtt
      541 gataacagta ataagaaaag gaagaataat gggggagaag ctcaataaac ccgtaagtaa
      601 tggcagaaag tgattgcaga aatattttct cggggccagg aagactggtt tttcatccgg
      661 atattgattt aaacaatttt cctgccagta cgggtttttc ttagttatga caattaaaag
      721 agctcaaggt ttgtgttcag ccggctggct gctttgttag atgccaaccc gcaagcgggg
      781 cagagagtgt ttaataaaat atttatacaa atgagagtac caatagagaa cggattaggt
      841 tttttcaacc aggtatcaga ttttattaag ctctctgcgg cttacaggtt ttttcttttg
      901 agttatcgta tttcgaattt tccggtagcg gtttcttcat cgccgctgaa aaccctgtac
      961 acataagtgc ctcttgaaag cccggttaga cggtagtggt cttttgcttt cattttgtaa
     1021 ttctgcacca gtgtgccctg caggtcaaat acgaagaaat caatttcttt tccatcattt
     1081 tctttagcta cgacatgcat gtctctcttt actgcatcgg gatagatctt tacagcactg
     1141 ttgttcagtg atgcaaagga agacttagcc ttcgttttat tttttttggc aggttcttct
     1201 tctgccggtg ccgttataga tattttcttt actggccttc cggaagctga ggcttgctgt
     1261 acaagcactg agccggaagc aaatgtaaac atacaggcta ctgccaggtg tcggtagtaa
     1321 gggtgtagtt tcatagtggg ttcggtttta aggtgatatt tcggattaat aatatactgg
     1381 acatttaagc tgcctcttgg cccttccatt aggtgtatta actccgttca acctccatcc
     1441 aaagcgcaga atgccggaaa agaaggcatc attctctttt ggatctcctc gttgctcatt
     1501 cgtgtacaca cggctaacag aagggttgta aatattttcc ctgaacatta attgccttgc
     1561 taccggtatt tgttcaggag cgagataatt attaaacaat gcagggtcta tataagtggt
     1621 gcttacatca tcaatataat cggtaaatgt tttacggtgc aggatctcaa aaccaacata
     1681 cataccatct gtaaggtagt atttaaccgc cacccactac aatctcaagc tgggtgagtg
     1741 aatattcctt acggtcagga tactcagcca ttccctgtcc ctcgaggcgg aggggtttta
     1801 gttccaccat tctcttgttg ccatttgatt cgatgaattc gcctttcggg ttgaagtgaa
     1861 atacaccaac cccacccaag ccataggggc gaattttgcc agccaacccg tcatattgtt
     1921 ccaggaaaac agtaggatat aactctaatg ccgcataggc ttcagtaatt tttgattcaa
     1981 acttcagatt tctctttctg cgaaatcttt ctgcgcctcc tttatctggc gcctgtgcat
     2041 catcaccctg aacatgtccc aggttaccgg ctaatcgaaa gcccagccac tccatgggat
     2101 agatgttaag gtaaattcct ttgctgatct tggtcattgg gagatcgaca tctttaataa
     2161 aggttttgcc aactccctcg cttccgccca aatcaccaag gaagatcatt ggcccaacgc
     2221 caatacctac ctcaattttc ccattgggtg aactaatcga ttgtgctgtg gaaagtagcg
     2281 agatgctaag aaatgtgctg acaagcacgg ataatcgtac agtgtgtaca aaggttctca
     2341 ttggtatcag atttctggtt tttcgactat tggattctcg caactaaggt aaaaagggac
     2401 gggagaataa gacaaacgtt ttgttatctt tttttgattc tcaccacttt aaaagttgtt
     2461 ctacttagtg attctacgaa aaacggatta tggttttacc atttcgatgt gttcaatgcc
     2521 atcttcaagg taaatatcac cagtgggttc gaagccgatt gaggagtaaa atttcttcag
     2581 gtataattgt gcgccgatac ggataggttg ttttccgaat agcttatata tagtatcaat
     2641 tgaatattcc atcagttctc ttcctgcacc agtacccctg gcagaaggag aagttacgac
     2701 ccttccaata gatggttcag cataggcagt tccggcagga atcaaccggg tataggcaat
     2761 gagcttgtcc tctttccagc ccataaggtg aaaagagtac ctgtctttat tatctgcatc
     2821 ctggtacaca caattttgct ctaccacaaa aacttcattg cgcaattgca tgatggtata
     2881 aagttcatgc ggagtcaact cttcgaattt tttcaatatc cattccatgc catgcaannn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnatgca ggtaaaagat ttaagtactg ttcatcaact tgccggtaaa ttattcagat
     3061 ggccatcatc aagaaaagaa tgggaaagct ataagttgag tgaggagcaa gtggcattct
     3121 tcagggagaa cgggtatctc gcaggggtga aaatgctgga tgaagatcag attgaagaga
     3181 tcaggaaaga gttagctgag atcgccgatc ccgcacatcc gggacatgct ttattttatg
     3241 aatttcactc caacgaatca acagatcctt ccaccatatt attccatgca ttaggagcct
     3301 ggcgcatcac tcccggtttg catgacgttt tatggaatcc gaggttcctg gtagcagcga
     3361 gtcaattgtt aggaaatgtg ccggtgcgat tctggcacga ccagttgttc tggaaacctg
     3421 caaagcaggg cggcgtggtg gcctggcacc aggattattc ctactggaca agaacaaagc
     3481 cgattgctca tctcacctgc tggtgtggcc ttgatgatgc aacaaaggaa aacggatgcc
     3541 tgcaatatat tcctggaagt catcagtggg gtttactacc aaaaccggta atagctggtg
     3601 aactggaggg tatcaaagat tatttaaatg aagatcaaag acagcaattt gaaaaacctc
     3661 aattggcaga agtaaaagcc ggggaagcgg tgtttcatca ttctctaaca ttgcatggtt
     3721 caggtgccaa tacttcccca aggccacgaa gggcatttgt gataaatgta tttgcagacg
     3781 gtgtcaggtc agatgctgat gagcctatgc tggatggtgt accttctgta cccagaggag
     3841 aaaaga
//
LOCUS       DAORLS010000288         3791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13189_length_3791_cov_2.226981, whole genome shotgun sequence.
ACCESSION   DAORLS010000288 DAORLS010000000
VERSION     DAORLS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13189_length_3791_cov_2.226981"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK977_16575"
     CDS             complement(<1..182)
                     /locus_tag="PK977_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRF19793.1"
                     /translation="METHKQLTIGLFGFGVVGEGLYKVLEQTPSLNASIKKVCIKNYG
                     KKRNAPASLFTTDKNE"
     gene            complement(169..1230)
                     /gene="metX"
                     /locus_tag="PK977_16580"
     CDS             complement(169..1230)
                     /gene="metX"
                     /locus_tag="PK977_16580"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRF19794.1"
                     /translation="MHEKFIHNRQFILESGISLPGLHLAYTTHGHMNDEKDNVIWIFH
                     ALTANSDPEEWWPGLVGKNKFFSPDRYFVVCANMPGSCYGSIGPLDTNPEINKPYYHN
                     FPIFTTRDMIRSYQLLKEHLGIGKIKIGIGGSMGGQQLLEWAIEEPALFESIIPIATN
                     AFHSPWGIAFNASQRLAIETDSTWKSNHVTAGIQGMRTARSIALLSYRNYQTYQWSQS
                     EEDTGKIDEFRSESYQRYQGEKLAMRFNAFSYYFLSKSMDAHNVGRNRQGVTAALNKI
                     KARTLIISISSDLLFPPDEQAFLAAHIPGAVYKAIDSDYGHDGFLLEYEQIEKAITEF
                     LDEESNPEPEIQKIINGNT"
     gene            complement(1232..2557)
                     /locus_tag="PK977_16585"
     CDS             complement(1232..2557)
                     /locus_tag="PK977_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRF19795.1"
                     /translation="MSSNLRFETLQLHAGQQIDPTTKSRAVPIYQTTSYGFNNAEHAA
                     NLFGLREFGNIYTRIMNPTTDVFEQRIAALEGGVAALATSSGQAAQFLALNNFLQAGD
                     NFITTSYLYGGTYNQFKVAFKRLGIEARFADGDDAESFVKLIDKNTKAFYLETIGNPR
                     LNIPDFQKFADLAKEYDLPLIVDNTFGAGGYLFRPLEHGANVVVESATKWIGGHGTTI
                     GGVIIDGGNYNWGNGKFPQFTEPSEGYHGLKFWDVFGEGNPLGLPNIAYAIRARVEGL
                     RDFGTSQSPFNAFLLLQGLETLSLRVQRHVDNALALAQWLEQHPAVEFVQYPGLKSNP
                     YNELAKKYLKYGFGGVLNFGIRGTKENASKFIDALKLVSHLANVGDAKTLAIHPASTT
                     HEQLSPEEQVASGVVPNQVRISVGIEHIDDIKADFEQAFEQVLAKQLVG"
     gene            complement(2790..>3791)
                     /locus_tag="PK977_16590"
     CDS             complement(2790..>3791)
                     /locus_tag="PK977_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRF19796.1"
                     /translation="ENGSTGIAFSSGLAAIDAVLKLLKAGDEIIAVDDIYGGAFRLFT
                     HIYEKFGIGVKYVDTTIAENVFNAVTPQTKLIWVETPTNPTLKISDIEAIGKIAKACQ
                     CILCVDNTFASPALQQPLSLGADIVVHSATKYLGGHSDLIAGLVVTKDKEVGEKIKFI
                     QNASGAILSPFDSWLVIRGIETLPLRIKQHCENARAVAEYLENHPLVEKVFYPGLKSH
                     PNHILATKQSKNFGGIVSFTLKDDSEGAATQFVTSTRYFKLAESLGGVKSLLCHPAQM
                     THKSIPVNKRKAAGVTDSLIRLSVGLEDVTDLINDLEQAFEKTKAKQLLVQDSQVNI"
BASE COUNT          995 a          802 c          802 g         1192 t
ORIGIN      
        1 aactcatttt tatcagttgt gaaaagcgat gcgggggcat ttcttttttt tccataattt
       61 ttgatgcata cttttttgat tgacgcattg agcgagggtg tttgttccag tactttatat
      121 agcccttcgc ctaccacgcc aaatccaaag agaccaattg ttaattgttt atgtgtttcc
      181 attaattatt ttttgtattt caggttcagg atttgattct tcatccagaa attcagtaat
      241 cgctttttca atttgttcat attctagtaa aaagccatca tgtccataat cagaatcaat
      301 ggctttgtaa actgcacctg gaatatgtgc agctaaaaac gcctgttcat ccggcggaaa
      361 cagcaggtcg ctgctgatac taataataag ggttcttgct tttattttat tgagagcagc
      421 agtcacgcct tgcctgtttc gcccaacatt atgtgcatcc atgcttttgc tgagaaaata
      481 gtagctgaac gcattaaatc gcattgccag tttttcaccc tggtaacgct gatatgattc
      541 acttctaaac tcatctattt tgccggtgtc ctcttctgac tggctccatt gataggtttg
      601 ataattgcga taggagagca gtgctatgct acgggctgtt cgcattccct ggatgccggc
      661 tgttacatgg ttacttttcc atgtactatc ggtttcaatg gctaatcttt gcgaggcatt
      721 aaatgctatt ccccagggag aatgaaaagc attggtggca attggtataa tactttcaaa
      781 caatgctggt tcttcgatgg cccattccag taattgttgt ccgcccatag agccgccaat
      841 tcctattttg atctttccta tgcccagatg ctcttttagc aactggtagg agcggatcat
      901 gtccctcgtg gtgaatatgg gaaagttatg gtagtaaggt ttattgattt caggatttgt
      961 atccaacggc ccaatactac cataacaact cccgggcata ttagcacaaa caacgaaata
     1021 gcgatcagga ctgaaaaatt tattctttcc aacaagtccc ggccaccatt cttctggatc
     1081 actgttagcg gttaaagcat gaaagatcca tatcacattg tctttttcgt cgttcatgtg
     1141 gccgtgtgtg gtgtaagcca aatgaagccc gggcaacgaa atgccggatt cgagtatgaa
     1201 ttgacgatta tgaatgaatt tctcatgcat gttagcccac taattgtttt gctaatactt
     1261 gttcaaaggc ttgctcaaaa tctgctttga tatcatcgat atgttcgatg cccacactga
     1321 tacggacctg gttgggaact actcctgatg ctacttgttc ttccggactt aattgttcat
     1381 gagttgtgga agcagggtgt atcgccaggg tcttggcatc accaacattt gctaagtgac
     1441 ttaccagttt cagtgcatca ataaatttgc tggcattttc tttcgttccc ctgatgccaa
     1501 aattcagaac acctccaaaa ccgtatttga gatatttttt agccagttca ttgtaggggt
     1561 tgctcttcag tccgggatat tgcacaaatt caacggcagg atgttgttcc agccattgcg
     1621 ctaaggcaag ggcattatca acgtgccgtt gtacccggag acttaaggtt tccagtccct
     1681 gcaataaaag gaatgcatta aaagggcttt gcgaagtacc aaaatcccgc agtccttcta
     1741 ccctggccct gatggcatag gcgatgttgg gcagcccaag aggattgccc tctccaaaaa
     1801 catcccagaa ctttagtccg tgataacctt ccgatggttc agtaaactga gggaacttgc
     1861 cgttgcccca gttataattt ccgccatcga taatgacacc gccgatggta gtaccatgcc
     1921 cgccgatcca tttagtggca gattctacaa ccacgtttgc accatgttcc agcggtcgga
     1981 ataaataacc gcctgcgcca aatgtattat caacaatcag cggcaaatcg tattctttag
     2041 cgagatcggc gaatttttga aaatctggta tgttcagtct tggattgcca atcgtttcta
     2101 gataaaatgc tttggtattc ttgtcgataa gctttacaaa actttccgca tcatcaccgt
     2161 cagcaaaccg ggcttcaatg cccaggcgtt taaaagctac cttaaactgg ttgtatgttc
     2221 ctccgtacag gtaagatgta gtaatgaagt tatcgccagc ctgcaggaaa ttgtttaagg
     2281 caaggaattg tgctgcctgc cctgaactgg ttgccaatgc agcgacgcca ccttccaggg
     2341 ctgcaatgcg ttgctcaaat acgtcggtag tggggttcat gatacgggta tagatattgc
     2401 caaattctct taagccaaaa agattggcgg catgttcggc attattaaag ccgtatgatg
     2461 tggtttgata aataggtaca gcccttgatt ttgttgtagg atcaatttgc tgaccggcat
     2521 gtaattgcag tgtttcaaaa cgaagattgc tactcatgga aatagttttt aagttttaca
     2581 aatggattct tcagaatggc aggcaataag agagggcgac aggcccgttg ggtaaagtat
     2641 tagtatgcct tacggcatac aacaacaaat acaacattgc tgtgtgatcg ggaagaaaat
     2701 attggttgtt ttccttttca tttgaatact ctacaaaaat aatagacttt aatttttatt
     2761 gccaaattaa ttttgtctga attatgtaat tatatgttaa cctggctatc ctgtactaac
     2821 agttgttttg ctttggtttt ttcaaaggcc tgttccaggt cattgatcag gtcagttaca
     2881 tcttcaaggc ctaccgataa tctaataagg ctgtctgtca cacctgctgc ttttctttta
     2941 ttgaccggga tggatttgtg cgtcatttgt gccggatgac ataaaagact tttgactcct
     3001 ccaaggcttt ctgctaattt gaaataacgg gtagatgtta caaactgcgt ggcagctcct
     3061 tctgaatcat ctttgagggt aaaggataca ataccaccaa aattcttact ttgttttgta
     3121 gcaaggatat ggtttgggtg gcttttcaag cccgggtaaa acactttttc gacgagaggg
     3181 tgattttcca ggtattctgc cacagcccgg gcattttcac aatgttgttt aatccggagt
     3241 ggcaatgtct caataccacg gattaccagc cagctgtcaa agggcgaaag gattgcgcca
     3301 cttgcatttt gaatgaattt aattttttcc cctacttctt tgtccttggt tactaccaaa
     3361 ccagcaatca gatcgctatg cccgcctagg tacttggtag cggagtgtac tacaatatca
     3421 gctcccagcg aaagcggctg ctgcagggcg ggtgaagcaa aagtattatc cacgcaaagt
     3481 atgcattgac atgcctttgc aatttttcca attgcttcaa tatcggaaat ttttaatgta
     3541 ggatttgttg gtgtttcaac ccagatcagt tttgtttgag gtgtaacggc attgaaaaca
     3601 ttttcggcaa tggtagtgtc gacatatttt accccgattc caaatttttc ataaatatgg
     3661 gtgaagagcc tgaaagcacc gccataaatg tcatcaacag caataatttc atccccggct
     3721 tttaataatt ttaagacggc atctattgct gctaatccgc tgctaaaagc aataccagtt
     3781 gatccatttt c
//
LOCUS       DAORLS010000289         3785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13213_length_3785_cov_1.923056, whole genome shotgun sequence.
ACCESSION   DAORLS010000289 DAORLS010000000
VERSION     DAORLS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13213_length_3785_cov_1.923056"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK977_16595"
     CDS             <1..212
                     /locus_tag="PK977_16595"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRF19797.1"
                     /translation="RFELGSYIDTAKVTLNEQLNRELVSGVRSYGNIKDIKLIGIYPM
                     QEHLVIRSNCTGELSVKVESIQFSL"
     assembly_gap    222..274
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<275..735)
                     /locus_tag="PK977_16600"
     CDS             complement(<275..735)
                     /locus_tag="PK977_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRF19798.1"
                     /translation="MRRHIHSARDFMLPIIDFFYPPFKRLMNIQTFRYLASGGGNTVL
                     GLLVYYISYKFIFFEKEFDFGFYAFKGHIAALIASFCVTFPVGFFMAKYVVFSDSNMK
                     GRVQLFRYLMVCLFNLALNYILLKIMVERFRIYPVLAQIITITIVIVFSYVA"
     assembly_gap    886..898
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <899..2603
                     /gene="argS"
                     /locus_tag="PK977_16605"
     CDS             <899..2603
                     /gene="argS"
                     /locus_tag="PK977_16605"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRF19799.1"
                     /translation="ESELTINSTKPEFEGDYTLVLFSFVKSLKKSPEQLGNEIGDALV
                     KAHPALFTGYNVIKGFLNLTVADSYWICFLQSNYNQRRFGSKPANGQKVMVEYSSPNT
                     NKPLHLGHLRNNFLGWSVAKILEESGYEIIKSCIVNDRGIHICKSMIAWQKFANGATP
                     QSTGVKGDHFVGEYYVKFNDAYKAEVDQLINEGMDKATAEKEAPIMKETQQMLQDWEA
                     GKPEVINLWNMMNSWVYEGFDVTYKRIGSDFQKMYYESDTYLLGKQFVEEGLQKGVFY
                     KKDDGSVWIDLTADGLDEKLVLRKDGTSVYITQDIGLAQQKYDEFKYDQSIYVIADEQ
                     NYHMKVLKLILQKLGKPYAEGVYHLSYGMVELPTGRMKSREGTVVDADDMVDEMVNVA
                     KQKTEELGKVKDFTEAELKELYDTIALGALKFFLLRVDPKKKMIFNPEESIDFHGFTG
                     PFVQYTHARIRSILRKSEVSNQKSAATGLLKLEKDIVIMLEQYPTILEQAVAEHNPSV
                     IAIYVFNLAKAFNTFYTEHSVMNAESEEKKQLRLQISEMTANVIASGMSLLGIRVPER
                     M"
     gene            complement(2767..>3405)
                     /locus_tag="PK977_16610"
     CDS             complement(2767..>3405)
                     /locus_tag="PK977_16610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF19800.1"
                     /translation="KAHYDMDNYGDAIKYLTMAAKEDPEKAEIPYMIGHSYADMTNYK
                     LAVPHFQKAIELDPTKNYWMYELGLICYAMHDDKNALKYILMAGEKGYKKDNDYFENL
                     GIAYLNIGDLDQGVAMLNEILKRKPSDLNILNMVAEAYYYKGRFDQAIEYWDKVLEYD
                     NTSASSLYMIGMAYQKKGGKENTEKGIRLCDKAIEMDPALASLKQKKMMMGM"
     assembly_gap    3406..3416
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3417..>3785)
                     /locus_tag="PK977_16615"
     CDS             complement(<3417..>3785)
                     /locus_tag="PK977_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19801.1"
                     /translation="SQTSVTSINLISVDSADFFLQKGLTEKQNGRRLESLKNFEKAAK
                     YDGNSKTIVAELASAYFDLRRYGQARDMYKKLVELGDQSAANYKQLLQLSFQLKQNED
                     VLLYADKLKAADPSEKVLYYV"
BASE COUNT         1115 a          745 c          837 g         1011 t
ORIGIN      
        1 cccggtttga attgggcagt tatattgata ctgccaaagt cactctcaat gaacaactga
       61 accgggaact ggtcagcggg gtgcgcagct atggaaatat caaagacatc aaactgatcg
      121 gcatttatcc catgcaggaa catttggtga tccgcagcaa ttgcaccggt gagttatctg
      181 taaaagtgga gtccatccaa ttcagcctgt agtttcttct tnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccaca taactgaaaa caatcacaat
      301 ggtgatcgta ataatttgcg ctaataccgg gtaaatgcgg aatctttcca ccattatttt
      361 cagtaatatg tagttcagag caaggttaaa caggcagacc atcaggtaac ggaatagctg
      421 caccctgcct ttcatattcg aatcgctgaa gacaacatat ttggccatga agaaaccgac
      481 cggaaaggtg acacagaaag atgcgatcaa cgctgcaata tggcctttga atgcataaaa
      541 tccgaaatca aactccttct caaagaagat gaatttgtag ctgatataat ataccagcaa
      601 cccaagtacc gtattaccgc caccggacgc caaataacga aaggtctgaa tgttcatcag
      661 ccttttaaag ggcggataaa agaaatcgat aataggcaac ataaagtccc gggcactatg
      721 tatgtggcgt cgcatgagca ggcaaaatac caaattccaa ataacaaatt ccaagttcct
      781 tagccggttt cgtaaatttg ccggctaaac acaaaacgga tgagtattgt accggaaata
      841 aaagtgaaag tggctgctgt gctgaaagaa ctgtatggca ttgaannnnn nnnnnnnncg
      901 aatcagaact gaccatcaac tccacgaagc cggaatttga aggcgattat accttggtat
      961 tgttttcttt tgttaagtca ttgaagaaat ctcccgaaca actgggtaac gaaataggtg
     1021 atgcattggt gaaagcccat cccgctttgt ttacggggta caatgtgatc aaaggttttt
     1081 taaacctcac cgttgcggac agttactgga tctgttttct tcaatcgaat tataaccagc
     1141 gccggtttgg ttctaaacct gccaacggtc agaaagtgat ggtagaatac tcctctccca
     1201 acaccaacaa acctttgcac ctgggccacc tgcggaataa tttcctgggc tggagtgtgg
     1261 caaagatcct ggaagaaagc ggttacgaaa tcatcaagag ttgtattgta aacgacaggg
     1321 gtattcatat ctgcaagagt atgatcgcct ggcaaaaatt tgctaatggt gccaccccgc
     1381 aaagtaccgg tgtaaaagga gatcattttg tgggtgagta ttatgtgaaa ttcaatgatg
     1441 cgtacaaagc agaagttgat cagctgatca atgaagggat ggataaagcc actgcagaaa
     1501 aagaagcccc tattatgaaa gaaacgcagc agatgctgca ggattgggaa gccggtaaac
     1561 cagaagtaat taatttgtgg aatatgatga acagctgggt gtacgaggga tttgatgtga
     1621 cctacaaacg catcgggagt gatttccaga aaatgtatta tgaaagtgat acctacctgc
     1681 tgggcaaaca atttgtagag gaaggattgc agaaaggagt tttctataaa aaagatgacg
     1741 gcagtgtgtg gattgacctg actgccgatg gactggatga aaaactggtg ttacgtaaag
     1801 acgggacatc cgtatatata acacaggata tcgggctggc acaacagaaa tatgatgaat
     1861 tcaaatatga ccagagcatt tatgtgatcg cagacgaaca gaactaccac atgaaagtgc
     1921 tcaaactgat attgcagaaa ttaggaaagc cctatgcaga aggagtttat catttaagct
     1981 atgggatggt agaactgcct accggcagaa tgaagagccg ggaaggaacg gtagttgatg
     2041 cagatgatat ggtagatgaa atggtgaatg tagcgaagca aaagacagaa gaactgggta
     2101 aggtaaaaga ctttacggaa gctgaactga aagagctcta tgataccata gcattaggtg
     2161 cattaaagtt cttcctgctg agggtcgacc cgaaaaagaa aatgatcttc aacccggaag
     2221 agtctatcga ctttcacggt ttcaccgggc cttttgtgca gtatacccat gcgaggatca
     2281 ggtctatctt aaggaagtct gaggtcagca atcagaagtc agcagctacc ggtttgctaa
     2341 agctggaaaa ggatattgtc atcatgctgg agcaatatcc cactatcctg gaacaagcgg
     2401 tggcagagca taatccttct gtgattgcca tctatgtatt caacctggcc aaagctttca
     2461 atactttcta caccgaacat tctgtgatga atgccgaatc agaagagaag aagcaactcc
     2521 gtttgcagat ttcagaaatg acagcgaatg tgattgcttc aggaatgagt ttattgggga
     2581 tcagggtacc tgagagaatg tgaggctgtt gccagtggat agtctttagt cgggagtcat
     2641 tagtcaggag ttaactatct gcaatttttt atattagaaa aagagctttc atcatttgaa
     2701 agctctttca atttatttgc atcttaatat tgactcagga ctatcaacta aaggctcccg
     2761 actccattac attcccatca tcatcttctt ctgtttcagg ctggccaggg caggatccat
     2821 ttcaatggct ttatcgcaaa gacggatgcc tttttccgta ttctctttac cgcctttttt
     2881 ctggtaagcc atgccgatca tatacaagga ggaggcgctg gtgttatcgt attccagtac
     2941 tttatcccag tactcaatag cctggtcaaa ccttcccttg taataatagg cttctgccac
     3001 catattgaga atgtttaaat cgctgggctt tcttttgagt atctcgttca gcatagccac
     3061 accctggtca aggtcgccga tattcaggta agcaattcca aggttttcaa agtaatcatt
     3121 gtctttctta tatccctttt caccggccat aagtatatac ttgagggcat tcttatcatc
     3181 atgcatagca tagcagataa gacccagttc atacatccag taatttttgg tgggatccag
     3241 ttcgatggct ttttgaaaat ggggaacagc cagtttgtaa ttagtcatat cagcatagct
     3301 gtgaccgatc atataaggaa tctctgcttt ttccggatct tctttggctg ccatggtaag
     3361 gtatttaatg gcatcgccat aattatccat atcataatgt gccttnnnnn nnnnnnacat
     3421 agtataatac tttttctgaa ggatcagccg ctttcagttt atcagcatac aataatacat
     3481 cttcattttg ctttaactga aacgaaagct gcaacaactg cttataattt gcggcagact
     3541 gatcgcccag ttctaccagt tttttgtaca tatcccttgc ctgtccgtag cgacgcaggt
     3601 caaagtaagc agaggccagt tccgcaacta tggttttgct gttgccatcg tatttggctg
     3661 ctttctcaaa atttttaagt gattcgagtc gtcggccgtt ttgtttctcc gtcaaacctt
     3721 tttgcaggaa aaagtcagcg ctgtctacac tgatcaggtt gatggaagta acggaagtct
     3781 gtgac
//
LOCUS       DAORLS010000290         3755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13327_length_3755_cov_2.034865, whole genome shotgun sequence.
ACCESSION   DAORLS010000290 DAORLS010000000
VERSION     DAORLS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13327_length_3755_cov_2.034865"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PK977_16620"
     CDS             <1..550
                     /locus_tag="PK977_16620"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRF19802.1"
                     /translation="LNLPKEKKTYLINPAWMAKLKEKAGEAKSFVKQKAYNNNYCFLI
                     DMSLPSGQNRFFIYNLKKDSIESSGLVAHGNCFEYWLEGRRYSNEVGSGCTSLGKYKI
                     GSPYTGKWGYSYKLHGLDSTNNNALERTVVLHSHSCVPDNEVADEICQSNGCPTVSPA
                     FLKKLKLIINSSKKPVLLWVYE"
     gene            complement(579..>830)
                     /locus_tag="PK977_16625"
     CDS             complement(579..>830)
                     /locus_tag="PK977_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HRF19803.1"
                     /translation="HAGCFSMKLSFVLGAAGFTLDSIETKCTITLESGAITSSHLEVK
                     ASVPGIDAGKFAECAADAKANCPISKLLNTEITMEAALA"
     assembly_gap    831..840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<841..997)
                     /locus_tag="PK977_16630"
     CDS             complement(<841..997)
                     /locus_tag="PK977_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HRF19804.1"
                     /translation="MKSFATANWKGSGKEGGGTLTSKSKVLDNTAYDFRSRFEDGTYT
                     NPEELVAA"
     gene            complement(1023..1205)
                     /locus_tag="PK977_16635"
     CDS             complement(1023..1205)
                     /locus_tag="PK977_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19805.1"
                     /translation="MRKFDRCENRVHAIFLFLSLLELVQQKFLKIMIGEGKNNFIIEY
                     NEPENRLAELEEQNQQ"
     gene            complement(1192..1806)
                     /locus_tag="PK977_16640"
     CDS             complement(1192..1806)
                     /locus_tag="PK977_16640"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRF19806.1"
                     /translation="MQNTSYQIKLPQFEGPFDLLLFFIERDELDIYNIPITNIINDFL
                     DYIHKSEELNIELSSEFILFISTLMRIKAKMLLPRKELDAQGNEIDPRQELVNKILEY
                     KRFKEASAQMAEMEALRMLMVKRGNIHRELSQIGEEAGEGTEIQNITLFKLMRAFERV
                     MQKYSDRLNKPVHTVVQYNYTMEGSRDQMLNMAREHKNVSFEKI"
     gene            complement(1907..2281)
                     /gene="mscL"
                     /locus_tag="PK977_16645"
     CDS             complement(1907..2281)
                     /gene="mscL"
                     /locus_tag="PK977_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRF19807.1"
                     /translation="MSMLKEFKEFAMKGNVIDLAVAVIIGGAFGAIVTAMVDKILMPI
                     IGSLIGKSFESLSATVNGVNIQYGSFIQAVVNFVIIAFFLFILIKAMNATKKKEEAAP
                     AAPPPTPEDIQLLREIRDALKK"
     gene            complement(2322..3065)
                     /locus_tag="PK977_16650"
     CDS             complement(2322..3065)
                     /locus_tag="PK977_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HRF19808.1"
                     /translation="MAVRLNEISTRAPEDFDKKQAKEKTARMVEELDDLQNLLFAEGK
                     HSVLIIIQGMDGSGKDGVIRNVLGNMNPQGVTVKSYKAPTQEELSHDFLWRIHAAAPG
                     KGMIQVFNRSHYEDILVTRVHKWCDDETAVKRMRAINEFEKLLQEHNNTHILKFYLHV
                     SPEEQHERLSERMKDPAKMWKYNEKDFEEAKLWDIYMKMYEDCFNNCNNPEWTIVPSD
                     QNWYKEYIIASKLHTLLKGLNMQFPGLKK"
     gene            3115..>3394
                     /locus_tag="PK977_16655"
     CDS             3115..>3394
                     /locus_tag="PK977_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19809.1"
                     /translation="MKPFIKINNNNSQYFVTFAHIFMSNKIVNWAVFILLCIIWGSSF
                     ILMKWGMYDKDMKPILSPWHVAALRMLSSGLVMLPFLFYSLKDLPKKAI"
     assembly_gap    3395..3436
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3437..>3755
                     /locus_tag="PK977_16660"
     CDS             <3437..>3755
                     /locus_tag="PK977_16660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRF19810.1"
                     /translation="FFPAFLFCIAETRIDGALTGSLNSLTPIFVILTGALFFGTRTSS
                     NKLIGVIVGLFGSFLLLYANISKPPEYLTYTGFVILATILYGINVNMVQNKLAGVSSV
                     QIAT"
BASE COUNT         1028 a          815 c          686 g         1174 t
ORIGIN      
        1 gctcaacctg ccaaaagaaa aaaagacgta tctaatcaac ccggcatgga tggcaaagct
       61 gaaggaaaag gccggcgaag caaaatcatt tgtaaagcag aaagcataca acaacaatta
      121 ctgttttttg attgatatga gcctgccttc agggcaaaac aggttcttta tttataacct
      181 gaaaaaagac agtattgaaa gttccgggtt agttgcacat ggtaattgtt ttgaatactg
      241 gctagagggc cggcgataca gcaatgaggt gggcagcggc tgtacatctt taggtaaata
      301 caaaataggc agcccttata ccggcaaatg gggctattcc tataaactgc atggattgga
      361 cagcaccaat aataatgcgc tggaaagaac tgtggtactt cacagtcaca gttgtgtacc
      421 tgataacgaa gtagcagatg agatttgcca gagcaatgga tgcccgacag tatcacctgc
      481 ttttctgaaa aaactgaaac ttataatcaa ttcatcaaaa aagccggtac tcctttgggt
      541 atacgagtaa accggcttca taaagttcca atccataatc aagccagggc agcttccatg
      601 gtgatctcag tattaagcag cttgctgatc gggcaatttg ccttggcatc agccgcacat
      661 tctgcaaatt taccggcatc tattccgggc actgatgcct ttacctcgag gtggcttgag
      721 gtaatggcac cgctttctaa ggtaatggtg catttagtct caatactgtc aagtgtaaac
      781 cctgcagcac ccaggacaaa gctcagtttc atggaaaaac aaccagcatg nnnnnnnnnn
      841 ctgctgctac cagttcttcc gggtttgtgt aagtaccatc ctcaaaacgg cttctgaaat
      901 cataagcagt attatccagc acttttgatt tggatgtcag tgttcctccg ccttctttac
      961 cagaaccttt ccagttggca gttgcaaagc ttttcataat taatcgtttt tattagtaga
     1021 atttattgtt gattttgttc ctccagttca gcaagcctgt tttcgggctc attgtactct
     1081 atgataaagt tattctttcc ttccccgatc attatcttta aaaatttttg ctgtaccagt
     1141 tcaagcaatg aaagaaacag gaatatggca tgcaccctgt tttcacaacg atcaaatttt
     1201 ctcaaaagaa acattcttat gctctcttgc catgttcaac atctggtccc ggctgccttc
     1261 catggtgtaa ttgtactgaa ccacagtatg aaccggcttg ttcagtctat cactgtattt
     1321 ctgcatcact ctttcaaaag ctctcataag cttgaaaaga gtgatattct ggatttctgt
     1381 tccttcaccg gcttcttcac cgatctgtga caattcccgg tgaatattgc ctcgcttaac
     1441 catcagcatc cgaagagcct ccatttcggc catttgcgca gaagcttctt taaagcgctt
     1501 atactccagt atcttattta ctagttcctg tcttggatct atctcattgc cctgggcatc
     1561 cagttcttta cgtggcaaca acatcttggc ctttattcgc atcaacgtag aaatgaaaag
     1621 aataaactca ctgcttagtt caatattgag ctcttcactt ttatggatgt agtcaagaaa
     1681 atcattgatg atattcgtta ttggaatatt ataaatatcc agttcatccc tttctatgaa
     1741 aaacaacaga agatcgaaag gcccttcaaa ttggggtaac ttaatctggt aagaagtatt
     1801 ctgcactttt ctaataaatt gattcagaaa aaaagtcctg tctttcaaca ggactttgca
     1861 aatctaaggg aaaacagcct tccggcagaa gggtcatttt ccacagttat ttcttcagtg
     1921 catccctgat ttcccgcaac aactgaatgt cttccggtgt tggtggtggc gctgccgggg
     1981 cagcttcttc ctttttcttt gtagcattca tggccttaat gagaataaaa aggaaaaagg
     2041 cgataattac aaagttgaca acagcctgta taaatgatcc gtattggata ttaactccat
     2101 taaccgttgc actaagtgac tcaaagcttt taccaatcaa tgatccgatt atcggcatca
     2161 gtatcttgtc taccattgct gtaacaatcg ctccgaaagc accacctata ataaccgcta
     2221 cagccaggtc gattacattc cctttcattg cgaattcctt gaattcttta agcatactca
     2281 taactgtaaa ttttagatgt gataaatgtt gttttttcta cttatttttt taatccggga
     2341 aactgcatat tcaaaccctt aagcaatgta tgtagcttgg atgcaattat gtactcttta
     2401 taccagtttt gatcggacgg aacgatagtc cattcaggat tattacaatt attgaagcaa
     2461 tcctcataca tcttcatata aatatcccac aatttcgctt cttcaaagtc tttttcattg
     2521 tatttccaca tttttgccgg gtccttcatc cgctctgaaa gcctttcatg ctgctcttca
     2581 ggagaaacat gcagataaaa cttcaggata tgagtattgt tatgttcctg caacagcttt
     2641 tcaaactcat tgatggctct cattcgttta acagccgttt catcatcaca ccacttatgc
     2701 actcttgtca ccaggatgtc ttcataatgc gaccggttga acacctgtat catgccttta
     2761 ccaggggcag cagcatgtat gcgccagaga aaatcatgtg atagctcctc ctgtgtgggg
     2821 gccttatagg atttcacagt aactccctgt ggattcatat ttcctaatac atttcgtatc
     2881 accccatcct taccgcttcc gtccattccc tggataatga tgagtaccga gtgtttaccc
     2941 tcagcaaata agaggttttg caaatcatcc agttcttcca ccatccgggc agttttttct
     3001 ttagcctgct ttttgtcaaa atcttcagga gctcttgtag aaatttcatt tagtcttact
     3061 gccattgcaa aaaatttact gaatgcaata tactatttca gcacagtgat ttatatgaaa
     3121 cctttcatca aaataaataa taataactcc cagtattttg tgacctttgc gcacattttt
     3181 atgagcaaca aaatagtaaa ctgggcagtt tttatcctgc tttgtatcat ttggggcagt
     3241 tccttcattt taatgaaatg gggaatgtat gataaggaca tgaagcctat tttgtcaccc
     3301 tggcatgtag cggcgttgcg gatgctttcg tcaggtttag taatgctgcc atttcttttc
     3361 tattcactta aagatcttcc taaaaaggct attgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnncttt ttcccggctt tcctgttttg catagctgaa acaaggatag
     3481 acggagccct tacaggcagc ctcaattccc taaccccgat ttttgtaatt cttactggcg
     3541 cccttttctt tggaacccgg accagtagta ataaactaat tggagttatt gttggcctgt
     3601 ttggaagttt tcttttgttg tatgccaata tcagtaaacc cccggaatac cttacctaca
     3661 caggtttcgt aatattagcc accattcttt atggaataaa tgtaaacatg gtgcagaata
     3721 agctggctgg tgttagttct gtacagattg ccacc
//
LOCUS       DAORLS010000291         3752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13344_length_3752_cov_2.265350, whole genome shotgun sequence.
ACCESSION   DAORLS010000291 DAORLS010000000
VERSION     DAORLS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13344_length_3752_cov_2.265350"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PK977_16665"
     CDS             <1..1337
                     /locus_tag="PK977_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF19811.1"
                     /translation="RDFDKRNPTYGLIVNPLGAGIYQNWARNVLNVENWNLSHKGSYD
                     KGKHAFLWGLGYDKSQINDKLNEWEFQDSAGYALPYQPNMLQLNKVLKSNASLDINKF
                     SGYLQDNILLGSDSTHSTTLSIGVRFNYNSLNQEFLVSPRIGASWKPKWKSDIIFRIS
                     AGAYNQPPFYRELRRNDGTINTSLKSQKSWQGVAGFDLNFVGIGGRPMRWTTEAYYKS
                     MTDVVPYDIDNVRIRYFGENNAKAYAAGVEMRLHGELVPDAESWISIGFMKTQEDING
                     DHYYRYKNAAGEFITAQSSDQVIADSARFDVGWLRRPTDRRITFGMYFQDYLPTNKNF
                     KMFLNLLYGSNLPYNIPGAVRYRNAAVIEPYIRADIGFSALLMDDDRSKRRSHSPFRN
                     FDNIWASLEIFNVIDRPNTISYLFIKDFSNTVYLLPNRLTPRLINFKIVARW"
     gene            1428..1964
                     /locus_tag="PK977_16670"
     CDS             1428..1964
                     /locus_tag="PK977_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19812.1"
                     /translation="MKYFSLITILLVLATISTLQAFAQVSNKKTWNISVGADIIYPEN
                     NFRKTHGWGYGGTIKAEYLFEKHASIILSTGAYTLSGKTSLLNPDARTVLGIPVKAGL
                     RYYLGNFYFGGDAGYLNQSGFESNNGFVYSFFIGDEIVTNKKNKNSLDLSIRHEAWVT
                     DRTRAFASIRLAYEFRVR"
     gene            complement(1980..>3431)
                     /locus_tag="PK977_16675"
     CDS             complement(1980..>3431)
                     /locus_tag="PK977_16675"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF19813.1"
                     /translation="TKKGKIYGETVVRFSDYQLRKNFLRGHFGLEVSSTSQEAYEKDS
                     TFWNAVRTEPLSKQQELYARYQDSIYRYMKSEAYLDSMDRVLNKITWKKALIFGQIFN
                     DHRKERMWILPPVTTLIQPVAFGGARLKLAAGYRKTYPNRTTLNWDADLSYGFRNSDI
                     NGTIGVQRKYNPFNKGMFGIKAGRNFEFIYAGDAWVNVLKRSNIFLNNSLELNHEIEL
                     FNGLTIMNTFEVALRRSVADYKVGNNADSLFGIANDPPVEFDPYNATYNQVKLYFTPR
                     MRYIREPKEKIHLGSRWPTFYVIWKKGIKGLLKSEIDFDYLEFGVEQNINFGVMGNTS
                     YNAKTGDFLNTNDLRVIDYKFMRQGDPLFFNNPKYAFQALDSTFPVFDRYFQGNLVHE
                     FNGALVNKIPFFKKLKIQEVAGGGMLITKERSLRYAEFFAGLERVFKWPFNPLTRLKL
                     GVYVVTSVANKFNNPVQFKIGLTTWDRFRNRWR"
     assembly_gap    3432..3444
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3445..>3752)
                     /locus_tag="PK977_16680"
     CDS             complement(<3445..>3752)
                     /locus_tag="PK977_16680"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF19814.1"
                     /translation="PVSKIPFVSPISYSGLMAYRFKTLKIDRTIKPKVYTIAIRPRQL
                     SNATIEGEIQIQDSTWNVLSVSFRIPPAHMPEYDYMEVKQEYDKIGDSARVITRQHFI
                     "
BASE COUNT         1062 a          796 c          777 g         1104 t
ORIGIN      
        1 aaagggattt tgacaagaga aatcctactt atggtttaat tgtaaacccg ttgggcgccg
       61 gcatttacca gaactgggca agaaatgtac tgaatgttga aaactggaat ctttcacata
      121 aaggaagcta tgataaaggc aaacatgctt ttttgtgggg attgggttat gacaaatcac
      181 aaatcaatga caagttaaat gaatgggaat tccaggactc agcagggtat gctttgccct
      241 atcagcccaa tatgcttcag ctaaacaagg tgttgaaatc aaatgcctcg cttgacatca
      301 ataaattctc cggttacttg caggataata ttttgctggg cagcgattcc acccattcca
      361 ctacattatc tattggcgtg cggttcaatt acaattcact caaccaggaa ttcctggttt
      421 caccgagaat cggcgccagc tggaaaccta agtggaaaag tgatatcatc ttccgtatct
      481 ctgccggtgc ttataaccag ccgccttttt accgggagct cagaagaaat gatggcacca
      541 tcaatacatc attaaaatcg caaaagagct ggcagggtgt ggccgggttt gacctgaatt
      601 tcgttggcat tggtggccgc cccatgcgat ggaccaccga agcttattat aagagtatga
      661 ctgatgtagt accttacgat attgacaatg tccgtatccg ctacttcgga gaaaacaatg
      721 ccaaagccta tgctgccggt gtggagatga gattgcatgg cgagttggtt cccgatgcgg
      781 aaagctggat cagcattggc tttatgaaaa cacaggagga tattaatggt gatcattact
      841 atcgatacaa aaatgcagca ggagaattca ttactgcaca aagcagtgac caggtaattg
      901 cagatagtgc ccggtttgat gtgggctggc tgcgccgtcc tactgaccgc cgcatcactt
      961 ttggtatgta tttccaggac tacctgccta ccaataagaa ttttaaaatg ttcctgaatt
     1021 tgctgtacgg cagcaatctt ccttataaca tacccggagc ggtccgctac cgaaatgctg
     1081 ctgtgattga accttatatc cgtgccgata ttggcttcag cgccttatta atggatgatg
     1141 accgcagtaa aagaagaagc cattctcctt tccggaattt tgataatatt tgggccagcc
     1201 ttgaaatttt taatgtgatt gacagaccta atacaatctc ttacttattc atcaaggatt
     1261 tttccaatac ggtttacctg ttacccaacc ggctgacgcc aaggctgatc aattttaaga
     1321 ttgtggcaag gtggtaaaac agccacaatt gccgggtctg cattttttta cttccttaat
     1381 tcaataaaca atcataaata actggtgtta ttagaaaaaa gtatatcatg aagtactttt
     1441 ctctaataac aatactgctt gtattggcta ctatttcaac cttgcaggct tttgcacagg
     1501 taagtaataa gaaaacctgg aatataagtg tgggtgcaga tattatttac ccggaaaata
     1561 attttagaaa aacacatggc tgggggtatg gaggtactat aaaagcagaa tacttatttg
     1621 aaaaacatgc cagcattatt ttatctaccg gtgcttatac tttatccgga aaaacaagtc
     1681 tgctcaatcc tgatgcaaga accgttttag gaatacctgt aaaagcaggt ttaagatatt
     1741 atcttggaaa tttctatttc gggggcgacg ccggttatct taatcaatcc ggctttgaat
     1801 caaataatgg ctttgtgtat tctttcttta taggagatga gattgtcaca aataagaaaa
     1861 acaaaaacag cctggatttg agcatacggc atgaagcatg ggtaacagac cgtaccaggg
     1921 catttgcctc tataagactg gcttacgaat tcagagtgag ataaggaaaa tatgattgtt
     1981 catctccatc ggttcctgaa cctgtcccat gtggtcagtc cgattttgaa ctgaaccggg
     2041 ttattgaatt tattcgccac agaagtaacc acatagactc ccagttttaa tcgggtcagc
     2101 gggttgaaag gccatttgaa aaccctttcc aggccggcaa agaactcggc gtatcgcaaa
     2161 cttctttctt tcgttatcag catgccgcca ccggccactt cctgtatttt tagtttttta
     2221 aagaacggta ttttgttcac caatgcccca ttgaattcat gcaccaggtt tccctggaaa
     2281 tacctgtcaa atactggaaa agtagaatcc agcgcctgga atgcatactt gggattatta
     2341 aagaaaagcg ggtcgccctg ccgcatgaat ttgtaatcaa ttacccgcag gtcattggta
     2401 ttgaggaaat cacctgtttt agcattataa ctggtattac ccatcacacc aaagttgata
     2461 ttctgttcta ctccgaattc caggtaatca aaatctattt cactcttcag caaacccttt
     2521 atgccttttt tccatatgac ataaaaagta ggccaccggg aacccagatg tattttctct
     2581 ttcggctccc tgatgtaccg catccgggga gtaaaataca atttcacctg gttgtaggtg
     2641 gcattgtaag gatcaaattc tacgggggga tcattggcaa taccaaaaag actgtcggcg
     2701 ttattaccca ctttataatc agccactgaa cggcgtaaag ccacttcaaa agtattcatg
     2761 atggtaagcc cgttgaacaa ctctatttca tgatttagtt ccaatgagtt gttcaaaaag
     2821 atattacttc tcttgagcac attcacccag gcatcacctg cataaatgaa ttcaaaattt
     2881 cgccctgctt taattccaaa cattccttta ttaaaaggat tgtatttacg ctgaacacct
     2941 attgtaccgt taatatcact gttgcgaaaa ccatagctca ggtctgcatc ccagttgaga
     3001 gtggtgcggt tgggataagt ttttcggtaa cccgctgcca gtttcaacct ggctccgcca
     3061 aatgccacgg gctgtatcaa agttgtaacc ggtggtagta tccacattct ttctttgcga
     3121 tgatcgttga agatttgtcc gaagatcaac gcctttttcc aggttatctt gttcagcacc
     3181 cgatccatag aatcgaggta agcctcactt ttcatgtacc ggtaaatgct gtcctggtag
     3241 cgggcataca attcctgttg ttttgacaag ggttctgttc gtaccgcatt ccaaaaagta
     3301 gaatcttttt cataggcttc ctgggaagtg gaacttactt ccagtccaaa atgccccctg
     3361 agaaaattct ttcttaactg gtaatctgaa aaccgcacaa ccgtttcgcc gtagatcttt
     3421 cctttttttg tnnnnnnnnn nnnnaaataa aatgctggcg ggtaatgacc cttgcactgt
     3481 caccaatttt atcatactcc tgttttactt ccatataatc atattcaggc atatgggcag
     3541 gtggtatccg aaatgagacg cttaaaacat tccatgtgct gtcctgtatc tgtatctcgc
     3601 cttcaatcgt tgcgttactc agttgccggg gccgtatagc aatagtgtat accttaggtt
     3661 taatggttct gtcaattttt aaagtcttga accggtatgc catcaggccg ctgtagctga
     3721 tgggagaaac aaaaggaatc tttgaaactg gc
//
LOCUS       DAORLS010000292         3732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13443_length_3732_cov_1.848246, whole genome shotgun sequence.
ACCESSION   DAORLS010000292 DAORLS010000000
VERSION     DAORLS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13443_length_3732_cov_1.848246"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PK977_16685"
     CDS             <1..1086
                     /locus_tag="PK977_16685"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRF19815.1"
                     /translation="MGKLYSQAIWIALGFSMAGIVITYLAAPFFLHTSLQSQVVEKQA
                     IQFLQIRIWGLPFLYLFQLSNAFLVSSNNSRYMKYAFIIEAGLNIFLDYGLIYGHFGL
                     PRLGFNGAAIASVIAEIVAVTVVLSIIYTRKFHLEFSLFKYWQYKKELAMLYFRQSLP
                     LIMQYLLSIVAWMLFYILIEHYGERPLAISNTMRNIFGVFGIFIWAFASTSNAMVSNI
                     IGQGKKEQVQLLIRKIMFLSLGFTSILCILMNIFPAIFLRLFSEDQTFIAEALPVIRM
                     VTIGVLSMSVATVWLNAVTGTGNTRMNLLIEIIAIFLYGIYVWQVLHVWKMSMVWAWA
                     SELLYWTVLFSLSYLYIRSGKWKNKII"
     assembly_gap    1157..1166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1167..1437)
                     /locus_tag="PK977_16690"
     CDS             complement(<1167..1437)
                     /locus_tag="PK977_16690"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRF19816.1"
                     /translation="MAGLMTIELTGLRFHAFHGLYEEEKIGGNEFEVSLYVNYCPQTE
                     IINELSGTINYAVIYDLVKTRMLQRTDLLETIAMSLAEDIHRQFPN"
     gene            complement(1446..2387)
                     /gene="trxB"
                     /locus_tag="PK977_16695"
     CDS             complement(1446..2387)
                     /gene="trxB"
                     /locus_tag="PK977_16695"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRF19817.1"
                     /translation="MESNERVQCLIIGSGPAGYTAAIYASRAGLKPVLYQGIQPWGQL
                     TITTEVENYPGYPDGIQGPEMMVHFEKQAARMGADIRYGLATKVDFSGPVHKVQIDEE
                     KWIEADSVIISTGASAKWLGLESEQRLNGYGVSACAVCDGFFFKGKEVAIVGAGDTAA
                     EEALYLSKICAQVHMIVRRDEMRASKVMQDRVKNTANIKIYWNSETDEVLGDKKVSAV
                     RIKNNKTGEKQEIPVSAFFVAIGHQPNSEIFKGWLDMDEAGYVKTVPGTSKTNIEGVF
                     AAGDVQDKIYRQAVTAAGSGCMAALDAERYLSAKGIH"
     gene            complement(2486..3373)
                     /locus_tag="PK977_16700"
     CDS             complement(2486..3373)
                     /locus_tag="PK977_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRF19818.1"
                     /translation="MKISTTLLLLLTVLFFSSCEKTVDFKLSLQEPKLVVEATIENGQ
                     APFVYLSRSIEYFSSVSYDILANSSVSGAEIYVSDGTRTHKLKEYSILTPTGSNFFYY
                     TNDPATPATAITGQLNSNYTLRITSNGKEYTASTRIPGITRQIDSVYWKPAPAGNPAE
                     KVVLMVKATDPAGFGDYIRYFTKQNRDPFYPGLNSVYDDQIIDGTTYEVQVERGIPRT
                     GSIPENYSFFNRGDTVTLKLANIDKATYDFWRTMEFTYASVGNPFSSPTKVISNVTGG
                     ALGYFGGYAAQYRTIIIPR"
     gene            complement(3504..>3732)
                     /locus_tag="PK977_16705"
     CDS             complement(3504..>3732)
                     /locus_tag="PK977_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRF19819.1"
                     /translation="DSLRNEIRRVLKSLSPREAEIVNAYFGLDGENGVTIEQIGQKYD
                     LTKERIRQIKERAIKRLQKARYSSALKAYLG"
BASE COUNT         1004 a          826 c          725 g         1167 t
ORIGIN      
        1 atggggaaac tatacagcca ggctatctgg attgcattgg gcttttccat ggcaggcatt
       61 gtaatcacat accttgcggc tccgtttttt ttgcatacca gcctgcagtc acaggttgta
      121 gaaaaacaag ccatccagtt cctgcaaatc aggatttggg gacttccttt cctgtatttg
      181 tttcagctat ccaatgcatt cttagtgagc agtaacaaca gccgctacat gaaatacgcc
      241 tttatcatcg aagcaggttt gaatattttt cttgattatg gattgatcta tggtcatttt
      301 ggcctgccca ggctgggatt taacggcgct gcaatagctt ctgttattgc agaaattgtt
      361 gctgtaactg ttgtactatc cattatctat accaggaaat ttcatttgga attcagtctt
      421 ttcaaatact ggcagtacaa aaaggaactg gcgatgcttt acttcaggca atctttgccc
      481 ttgatcatgc agtacttgct gagcattgtg gcctggatgc ttttttatat cctgattgag
      541 cattatggcg aaagaccgct tgccattagt aatacaatgc gaaatatatt tggagtgttt
      601 ggcattttta tatgggcttt tgccagcacc agtaatgcta tggttagcaa tataattgga
      661 cagggcaaaa aagagcaggt acagctgctt atcagaaaga ttatgttttt aagcctgggt
      721 tttaccagca ttctgtgcat attgatgaat attttccccg ctatttttct acgtctattc
      781 agcgaggatc aaacatttat tgcggaggcc cttcctgtaa tcaggatggt gacaattggt
      841 gtactgagta tgtcagtagc aacagtttgg ctgaatgcag ttaccggtac cggaaatacg
      901 agaatgaatt tactcataga aattatagcc atatttcttt atggcattta cgtctggcag
      961 gtgttacatg tctggaaaat gtctatggta tgggcatggg cttcggaact gctgtactgg
     1021 acagtcttat tctccctttc gtatttgtat atcaggagcg gtaaatggaa aaataaaata
     1081 atttaaaatt ccttctggta cctgaccgcc acagtaccgt tgaagccagc aataggcgga
     1141 tgaagctttg ctattgnnnn nnnnnncatt cggaaactgc cggtggatat cctctgccag
     1201 ggacattgca atcgtttcaa ggagatcagt tctttgcagc attctcgttt tcaccaaatc
     1261 ataaataacc gcataattga tggtgcctga aagttcattt atgatctctg tttggggaca
     1321 ataattgaca tacaagctga cctcaaactc attgccccca atcttttctt cctcatacaa
     1381 gccatgaaat gcatgaaagc gaagaccagt gagttcgatt gtcattaatc cggccataat
     1441 acattttagt gaattccttt ggcgctaagg tagcgttctg catccaaagc agccatacaa
     1501 ccactgccgg ccgctgtcac tgcctggcga taaattttat cctgaacatc acccgcagca
     1561 aaaacacctt caatattggt tttacttgta ccgggcactg tttttacata ccctgcctca
     1621 tccatatcca gccaaccttt gaaaatttca gaatttggct gatgaccaat tgctacgaaa
     1681 aaagcactaa ccggaatttc ctgtttttcc cccgttttat tattttttat tcttactgcc
     1741 gatacttttt tatctcccag cacctcatcc gtttcgctat tccagtaaat tttgatgttg
     1801 gccgtattct ttaccctatc ctgcattact ttactggctc tcatttcatc ccttcgaaca
     1861 atcatgtgta cctgtgcaca aattttagag agatacaaag cttcttctgc agcggtatca
     1921 cctgccccga caatagctac ctctttccct ttaaagaaaa aaccatcgca tacggcacag
     1981 gcactgacac cgtaaccgtt taatcgctgc tcactttcca aacccagcca ttttgcagat
     2041 gcccccgtcg agatgatcac tgaatctgct tctatccatt tttcttcatc aatttgtact
     2101 ttatgtaccg gtccggaaaa atcaacttta gtggctaaac cataccggat atctgccccc
     2161 attctggctg cctgtttttc aaaatgtacc atcatttcag gaccctgtat gccatcagga
     2221 tagccaggat aattttcaac ttctgtagta attgttaact gaccccaggg ctgaataccc
     2281 tggtacaaaa ctggcttcaa ccctgccctg cttgcataaa tagctgctgt atatcctgcc
     2341 gggcctgaac cgataatcag gcattgaact ctttcgtttg attccatact ggaaaattta
     2401 tatccggcaa aaatagccgt taaaactatt taattaatgt gacattagtc gccctgttga
     2461 ttactgtgga caaaccttaa tttgtttacc gtggaatgat aatagttctg tactgcgctg
     2521 cgtatcctcc aaaatacccc agcgcaccac ctgttacatt actgatcact ttggtcggag
     2581 atgaaaaagg atttccaaca cttgcgtagg taaattccat tgttcgccaa aaatcgtatg
     2641 tggccttatc tatattagcc agcttgaggg ttacagtatc gccacgatta aaaaatgaat
     2701 agttttcagg tatagagccg gttctgggaa tccctctttc cacctgcact tcataggttg
     2761 taccgtcaat aatctggtca tcatatactg aatttaaccc gggataaaaa gggtctctgt
     2821 tttgctttgt aaaatacctg atataatcac caaaaccggc aggatctgtc gctttgacca
     2881 ttaaaactac tttttcagcg gggtttccag caggtgcagg tttccagtaa acagaatcaa
     2941 tttgccttgt tattcccgga atccttgttg aagcagtgta ttcttttcca ttactggtta
     3001 ttcttaatgt atagtttgag tttagttgcc ctgttatcgc agttgcagga gtggccgggt
     3061 cattcgtgta gtaaaaaaaa ttgctgcctg tcggtgttag aatactgtat tccttcaatt
     3121 tatgtgttcg ggtaccgtca gatacatata tttctgcccc ggaaactgaa ctattggcta
     3181 aaatatcgta ggatactgaa gaaaagtatt caatacttcg ggacaaataa acgaatgggg
     3241 cttgtccatt ttcaatagtg gcttccacta ccagttttgg ctcctgcaaa ctcagtttaa
     3301 agtcaactgt cttttcacag gatgaaaaaa acaaaaccgt aagcaaaagc aataatgtcg
     3361 ttgaaatttt catagccagt gtaaaaataa cccgccaagt gtgaaatagt ttggaaagaa
     3421 gaactccggt ctcatttgaa caagaccgga gttaaagtac tgatataccc accaacctgc
     3481 ctccgcaggc agattttcat aatttagccg agatatgctt ttaaagcact gctgtaacgg
     3541 gccttttgta agcgcttaat agccctttct ttgatctggc ggatacgttc ctttgtcaga
     3601 tcgtatttct gaccaatttg ctcaatggtt accccatttt cgccatccag gccaaagtaa
     3661 gcattaacaa tctcagcttc ccggggagaa agtgatttta acacccggcg aatttcgttt
     3721 ctgagagaat cc
//
LOCUS       DAORLS010000293         3717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13521_length_3717_cov_1.751502, whole genome shotgun sequence.
ACCESSION   DAORLS010000293 DAORLS010000000
VERSION     DAORLS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13521_length_3717_cov_1.751502"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PK977_16710"
     CDS             <1..662
                     /locus_tag="PK977_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19820.1"
                     /translation="YGVNENTAIRKIAAALDEQATLSIKATTVYGGSQQDDIHGMINN
                     LSKDKVKEYLHEELDFATYEVTAFDYKESKSSNPLIEEKLDIQVSNYATMSGRRLFIL
                     PNVMTRSHRKMAADENRKYPVILNYAYTDIDTVEINIPAGYTAESMPQEVSLDTRFGK
                     YRSKVKLEADKLIYYRSMQAFSGRFEPAAYNELVKFYETIYKADRSRVVLVKNEQTKG
                     F"
     gene            complement(663..1796)
                     /locus_tag="PK977_16715"
     CDS             complement(663..1796)
                     /locus_tag="PK977_16715"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF19821.1"
                     /translation="MSYKVSSICMKVIFVAGFLLFFQPVFSQYNWSELDNELAAKQKL
                     LGQDLVAMIWKGDSLVYKKEMGSFNSKTQAPIASCSKWLTAALVMQLVDEGKLSLDDK
                     VAKYIPEFDKYFKGYITIRHCLSHMTGIEDDGKLIKRLLERRKFASLEEEVNSFAARD
                     IRANTGTDFWYGNIGLNIAGRVAEVITRKKFDVLIRTKLFIPLGMTKTTFASLNGAPI
                     NPSGGARSTADDYMKFLVMLLNKGKYNGVQILSENAVNEMLQPQNRLEQVKYTPKSAE
                     GFRYALGAWVIEEKGKQVTYVDGKLHTGPLVPPPTGNVETKSITTANAIASPGLFGTW
                     PMIDYCRGYTYIVFVKNLLGEERADAHLQLKKIVDAQTSSSCK"
     gene            1928..>2822
                     /locus_tag="PK977_16720"
     CDS             1928..>2822
                     /locus_tag="PK977_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRF19822.1"
                     /translation="MNLGNFTIKAAEAFQQAQQIAFNAQSPNIETEHILKALLDQPDS
                     PVEYLLKKNNVTINLVENKLDELISKLPKASGDAAQQISRDANNVVLRAGAVLKQFGD
                     EFVTPEHILLAIVQGNDAAAKLLKNAGLSENGLVTAIKELRKGEKITSQTQSQEFNAL
                     NKYAKNLNELARQGKLDPVIGRDEEIRRTLHILSRRTKNNPILVGEPGVGKTAIAEGI
                     AHRIVNGDVPENLKSKIIYALDMGLLIAGAKYKGEFEERLKAVVKEVSSSDGEIILFI
                     DEIHTLVGAGGGDGAMDAANIL"
     assembly_gap    2823..2892
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2893..>3227
                     /locus_tag="PK977_16725"
     CDS             <2893..>3227
                     /locus_tag="PK977_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HRF19823.1"
                     /translation="QKFFEKDKALERRFQKVMVDEPSVEDAISILRGLKDRYETHHHV
                     RIKDEAIIAAVELSSRYITDRFLPDKAIDLIDESAAKLRLEMNSMPEELDTLERQIRQ
                     LEIEREAIK"
     assembly_gap    3228..3434
                     /estimated_length=207
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3435..>3717
                     /locus_tag="PK977_16730"
     CDS             <3435..>3717
                     /locus_tag="PK977_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HRF19824.1"
                     /translation="YGKIKEQETLINSLTTQLLNATEKRLLKEEVDAEDIAESIAKAT
                     GIPVQKMLQSDREKLLHLEEHLHERVVGQEEAITAVADAIRRSRAGLQDP"
BASE COUNT         1020 a          795 c          789 g          836 t
ORIGIN      
        1 tttatggagt aaatgagaac actgctatcc ggaaaatagc agccgcactg gatgagcagg
       61 ctacactcag cataaaggca acaaccgtgt atggcggcag ccagcaggat gatattcatg
      121 gtatgatcaa caacctgtcg aaagataaag tgaaggaata cctgcacgag gaactggact
      181 ttgctaccta tgaagtaact gcttttgatt ataaggaaag caaatcctcc aatccgttga
      241 ttgaagaaaa actggatata caggtaagca actacgctac catgagtggc cgtcgtttat
      301 tcatactgcc caatgtaatg acaagaagcc accgcaaaat ggcagcggat gaaaacagga
      361 agtatcctgt tatacttaac tatgcctaca ctgatattga cactgttgaa ataaatattc
      421 ctgccggcta cactgcggaa agcatgccgc aggaagtatc tcttgatacc cgttttggca
      481 aataccgttc gaaagtgaag ctggaagcag ataagctgat ctactaccgc agcatgcagg
      541 ccttcagcgg ccgctttgaa cctgcggctt acaatgaact ggtaaaattc tacgagacta
      601 tttacaaagc agaccgcagc cgggtagtgc tggtaaagaa tgaacagacg aaggggttct
      661 gattatttac agctgctact ggtttgtgca tctacgatct ttttaagttg taaatgtgca
      721 tccgcccttt cttcacccag caggtttttt acaaatacaa tataggtata gccccggcag
      781 taatcaatca tgggccaggt accaaacaga cccggtgaag ctatagcgtt tgcagttgta
      841 atggatttgg tttccacatt accggtgggg ggaggtacca ggggcccagt atgaagtttc
      901 ccgtcaacat atgtaacctg tttccctttt tcttctatca cccaggcacc cagcgcatag
      961 cgaaaacctt cggctgattt aggcgtatac ttcacctgct ctaaacggtt ctgcggttgc
     1021 agcatttcat tcaccgcatt ttcgctgagg atctgcacac cattgtactt tcctttgttc
     1081 agcagcatca ccaaaaactt catatagtca tccgcagttg accttgcccc gcctgatgga
     1141 ttaatgggcg caccgttcag tgaggcaaat gtagtcttcg tcatacccag cggaatgaac
     1201 agttttgtcc ggatcagtac atcaaacttt ttcctggtga tcacttctgc cacacggccg
     1261 gcaatattca gcccgatatt gccataccag aaatcagtac cggtattggc ccggatatca
     1321 cgggcagcaa aactgttcac ttcttcttcc agcgaagcaa actttcttcg ttccagcaat
     1381 cgctttatca gtttgccatc atcttcaatt cccgtcatat ggctcaggca atgccggata
     1441 gtgatataac ctttaaaata tttatcaaat tcaggaatgt atttcgccac cttatcatcc
     1501 agcgacaatt tgccctcgtc caccagctgc atcaccagtg ccgctgtcag ccatttactg
     1561 cagctggcaa taggtgcctg tgtcttgctg ttgaagctgc ccatttcttt tttatacacc
     1621 agggaatcac ctttccatat catcgccacc aagtcctgcc ccagcagttt ttgtttggcc
     1681 gccagttcat tatccagttc cgaccagttg tattgagaaa agacgggctg aaagaatagc
     1741 aggaaacccg ctacaaatat gactttcatg cagatagaac taactttata tgacatagtt
     1801 gctgtgtttg tggccttgga taagtatttg agtgtacagt ttaaaattat agaatatcaa
     1861 cacgaaggta ttatggtgtt aaaaatatgt tgtgccttag tgtctttgtg ggagaaaaaa
     1921 ataaaatatg aacttaggaa attttaccat aaaagcagcc gaggcctttc agcaggcaca
     1981 gcaaattgct ttcaacgcac aaagcccgaa tattgagacc gagcatatct taaaagcgtt
     2041 gctcgatcag cccgattcgc cggtggagta tttgctaaag aaaaacaatg ttaccatcaa
     2101 cctggtggaa aataaactcg atgaactcat cagcaaactg cccaaagctt ccggtgatgc
     2161 agcacagcag atcagccggg atgccaataa tgtagtgttg cgggcagggg cagtgttgaa
     2221 acagttcggt gatgaattcg taacaccgga gcatattttg ttagccatag tgcagggcaa
     2281 tgatgccgct gccaaattac tgaagaatgc cggcctttct gaaaacggcc tggttacggc
     2341 tatcaaagaa ttacgcaaag gcgaaaagat tacttcgcaa acacagtcgc aggaattcaa
     2401 tgcactgaat aaatatgcga agaacttaaa tgaactggcc aggcagggaa aacttgatcc
     2461 cgttatcggc cgtgatgaag agataagaag aaccctgcac atactttccc gccggacaaa
     2521 gaacaacccc atactcgttg gtgaacccgg tgtgggtaaa acagccatcg ccgaaggcat
     2581 tgcacatcgt attgtaaacg gtgatgtgcc cgagaattta aaaagcaaga tcatctatgc
     2641 actcgatatg ggcctgctca ttgccggtgc caagtacaaa ggtgagtttg aagaaaggct
     2701 gaaagctgta gtgaaagaag tgagcagcag cgacggcgag atcattttat tcattgatga
     2761 aatacatacc ctggtaggtg caggcggtgg ggacggcgct atggatgccg ccaatatcct
     2821 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnaccagaaa ttctttgaaa aagataaagc actggaacgc cgtttccaga
     2941 aagtgatggt ggatgaacca tccgtggaag atgctatctc tatcctccgg ggtttgaaag
     3001 accgttacga aacacaccac catgtacgca tcaaggatga agccatcatt gcggccgtgg
     3061 aattatccag ccgctatatc accgatcgtt tcctgccgga taaagcgatt gacttaatcg
     3121 atgaaagtgc tgccaaactt cgcctggaga tgaactccat gcccgaagaa ctggatacat
     3181 tagaaagaca gatccgtcag ttagaaatag aaagagaagc gatcaaannn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnctatgg aaaaataaaa gaacaggaaa cccttatcaa ctccttaact
     3481 actcaacttc tcaacgcaac cgagaagcgg ttgctcaaag aagaagtcga cgccgaagac
     3541 attgccgaaa gcattgccaa ggctaccggc atcccggtac aaaagatgct gcagagcgac
     3601 agggaaaaac tcctgcacct cgaggagcat ttgcatgaaa gggtagtggg acaggaagaa
     3661 gccatcaccg ccgtagctga tgccatacgc cgcagcaggg caggtttaca ggatccg
//
LOCUS       DAORLS010000294         3716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13527_length_3716_cov_1.725758, whole genome shotgun sequence.
ACCESSION   DAORLS010000294 DAORLS010000000
VERSION     DAORLS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13527_length_3716_cov_1.725758"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>234
                     /locus_tag="PK977_16735"
     CDS             42..>234
                     /locus_tag="PK977_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19825.1"
                     /translation="MRKIYKLSFTVFALFVLTLSLEAQNYFFTDAGENRLLTTTGQRV
                     IIPQKFRTSSLDVQSMKSFL"
     assembly_gap    235..381
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <382..>587
                     /locus_tag="PK977_16740"
     CDS             <382..>587
                     /locus_tag="PK977_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19826.1"
                     /translation="TFLGQGIDDPYATIRMDFGPNGFHAQVLTANGTYYIDPYARGEV
                     GNYISYFRTDLQKAGTWLCEVPDN"
     assembly_gap    588..639
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            640..>1707
                     /locus_tag="PK977_16745"
     CDS             640..>1707
                     /locus_tag="PK977_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HRF19827.1"
                     /translation="MRTYRLAVACTGEYAQAPGIAAGSNPAILHNAIVTTVNRVTGVY
                     ETEVSVRMVLVANNNLIEFLNAATDPFTGNNNANVLINESQTVIDANIGSANYDIGHT
                     FSTGGGGLAQLSCVCGSSKARGITGSPNPTGDAYDIDYVAHEMGHQFGGNHSMAGCGS
                     SPASTKYEVGSGTTIQAYAGICGAENIQPNSDPFFHAISFDEISNFVNGAGGTCAVLT
                     PTGNTLPVIDPLPNNGVTIPISTPFTLSGTATDANGDALTYCWEQWDLQGVQTWNAGA
                     TAPAGNTVPLFKSRVPKTNGNRTFPDIAVILANYPANPPATMGGLKGETLSPVARAMN
                     FRLTVRDNRAGGGGVSSSGGGG"
     assembly_gap    1708..1717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1718..3117
                     /locus_tag="PK977_16750"
     CDS             <1718..3117
                     /locus_tag="PK977_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19828.1"
                     /translation="QTSTAFTINVAGTAPFVVTSPNGGESYAGGSTQTVTWNVVGTNA
                     APFNVSNVRILFSNDGGLTFPTVLLASTANDGSESVTIPVGVTSTARIKVEALGNIFF
                     DISNNNFSVTAPVNGFTFNSPAAVIATCPAPNPMTSGNLTATYVGSFTGNINLSAASV
                     PALPGGATISFSNNPLTTGSPSTTVSLNGMQNASPGTYTITVTGVGVSGPTVTRDITF
                     TINPGSGPSVTTQPSNQTVCVGQNATYSIVATGVTGYQWQVSTTGCGGTFTNISGATS
                     ASYTVTAATAGQNGYAYRCILTGQCGTTTSNCATLTVNTAPAITGQPANISVCSGSAA
                     SFTVTATGSGIGYQWELSTDGGTTWNPIGGATNATYTIASTTTGQNGYQYRCVVTGTC
                     TPSATSSAAVLTVNSSVTVTGNPSNSTICEGTGTSFSASASGTGLNYQWRRNLEQCGE
                     QCQLQRRYHAYTDTD"
     gene            3062..>3716
                     /locus_tag="PK977_16755"
     CDS             3062..>3716
                     /locus_tag="PK977_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19829.1"
                     /translation="MVNNANYSGATTPTLTLTNTPPAFNGYRYRCNITSPPCTPGITT
                     SALLTVNTFPVITAQPANTSICEGGNPTFSVTATTAVGSLSYQWQVSTNGGVSWTNIA
                     GATSNSYSQTNIPVGQNGYRYRVIVTAGCGSSTSNSATITVNAYPVATLTNIPVTLCL
                     SDPSYSLTASTAGGTWSGAGVTGSSFSPSAAGLGTKTISYTLNNAGCVTTKTSIIQVN
                     "
BASE COUNT          969 a          799 c          811 g          928 t
ORIGIN      
        1 tattcagggt cctgggatat tttatcaaaa ccatttaatt tatgagaaaa atctacaagc
       61 tcagctttac ggtttttgcg cttttcgttc tcactttgtc actggaagca cagaattatt
      121 ttttcaccga tgccggtgaa aacaggcttt tgactactac cggacaaaga gtaataatac
      181 cgcaaaaatt cagaacatca tctttggatg ttcagtcaat gaagtcattt ttatnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn naactttcct tggccagggt atagatgatc cttatgctac
      421 aattcgtatg gatttcgggc ctaatgggtt tcatgcccag gtattaacag ctaacggcac
      481 ttattatatt gatccttatg caagaggtga agttggtaat tatatcagct atttcagaac
      541 tgatcttcag aaagcaggaa catggctctg tgaagtccct gataatcnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgagaactta tcgtttggca
      661 gttgcgtgta ccggtgaata tgcacaggct ccgggtattg cagcaggttc aaatcctgcc
      721 atactgcaca atgcaatagt aaccacggtg aacagggtaa caggggtata cgaaactgag
      781 gtttctgtaa gaatggtttt ggtcgcaaat aataacctta ttgagttttt gaatgctgca
      841 acagatccat tcactggaaa taataatgca aatgtgctta tcaatgaaag tcagacggtg
      901 attgatgcca atatcggttc tgccaactat gatataggtc acacatttag tacaggtggt
      961 ggtggattag cacaattgag ttgtgtatgc ggatccagca aagcaagagg tattacggga
     1021 agtcctaatc ccacaggaga tgcttatgat attgattatg ttgcccacga aatgggacac
     1081 caatttggtg gtaatcattc tatggcaggt tgtggttctt cacctgcctc taccaaatat
     1141 gaagtgggaa gcggaaccac tatccaggct tatgccggta tatgcggagc agaaaacata
     1201 caaccaaaca gtgatccatt ctttcatgct atcagctttg atgaaataag caattttgtc
     1261 aatggcgccg gtggtacctg tgctgttctt acgccaaccg gtaatacact tcccgttata
     1321 gatccattac cgaataatgg tgttactata cccatcagta ctccctttac actatccgga
     1381 actgcaactg atgctaatgg tgatgcattg acctattgct gggagcaatg ggatttgcag
     1441 ggtgtgcaga cctggaatgc gggtgctaca gcgcctgcag gtaatactgt gccccttttc
     1501 aaatcaaggg taccaaaaac aaacggtaac agaacattcc ctgatattgc cgtgatttta
     1561 gcaaactatc ctgctaaccc tccggctacc atgggtggtt taaaaggtga aacactttca
     1621 ccggttgccc gtgccatgaa tttcagactt accgtaagag ataacagggc cggtggcggc
     1681 ggcgtatcat ccagtggagg cggtggtnnn nnnnnnngcc agacatccac agcattcaca
     1741 attaatgttg cgggcactgc accttttgtt gttacatcgc ctaacggcgg tgaaagctat
     1801 gccggtggat caacccaaac agttacctgg aatgtggtag gtaccaatgc ggcccctttc
     1861 aatgtgtcaa atgtgagaat acttttttcc aatgatggtg gcttgacatt cccaaccgtt
     1921 ttattggcta gcactgctaa tgatggttca gagtctgtaa caattccggt cggagttact
     1981 tccactgccc gtattaaagt ggaggcattg ggaaatatat tctttgatat ctccaataat
     2041 aatttcagtg taactgcacc agttaacgga tttacattta atagccctgc tgcagtaata
     2101 gctacatgcc cggcgcctaa tccaatgact tccggaaatc tgacggctac atatgtagga
     2161 tcatttactg gtaacattaa cctaagtgcg gcatctgtac cggcattgcc aggcggcgct
     2221 accatatcat ttagcaacaa tccattaacc actggttcgc catctacaac tgtatctctt
     2281 aatggaatgc agaatgcatc tcccggcaca tatactatta ccgtaactgg tgttggtgta
     2341 agtggaccca ctgtaacaag agatatcact ttcacgatca atccgggttc aggtccatcg
     2401 gttacgacac agccatcgaa tcagacagta tgtgtgggtc agaatgcgac ttatagcata
     2461 gtagcgacag gcgtaaccgg ttaccagtgg caggtaagca caacaggatg tggcggtacc
     2521 tttaccaata tatccggtgc tacatcagcc agttatacgg taacggcagc cacagccggc
     2581 cagaatggtt atgcatatcg ttgtatactt acaggccagt gcggaaccac gaccagcaat
     2641 tgtgctacat taacagtaaa taccgcacca gccattacgg gtcagcctgc gaacatcagt
     2701 gtatgttcag gaagtgctgc ttcctttaca gtaactgcca caggatcagg aattggttat
     2761 cagtgggagt taagtacaga tggtggtaca acctggaacc cgattggagg agctaccaat
     2821 gcgacttata caatagcgtc cacaacaaca ggtcagaatg gttaccagta tcgttgcgtg
     2881 gttacaggta cctgtacacc ttcagcgact tcatcggctg cggtgcttac ggtgaattca
     2941 tcagtaacgg taacaggcaa tccttccaac agtaccattt gtgaaggaac cggtaccagc
     3001 tttagtgcat cagccagcgg tacaggtctc aactaccaat ggcggcgcaa cctggagcaa
     3061 tgtggtgaac aatgccaact acagcggcgc taccacgcct acactgacac tgactaatac
     3121 acctcctgca tttaatggtt atcgctatcg ttgcaatatc accagtcctc catgtacacc
     3181 tggtattaca acatcagcct tgctgacagt aaatacattc cctgtaatta cggcacagcc
     3241 ggcgaacacc agtatatgtg aaggtggtaa cccgaccttc tccgtaacgg ctaccacagc
     3301 tgtgggttca ttgagctacc agtggcaggt aagcaccaac ggcggagtaa gctggacaaa
     3361 tattgcggga gctacgagca acagctattc ccagaccaat attcctgtag gtcagaatgg
     3421 ataccgttat agggtgattg taacagccgg ttgcggatca tctacttcaa attctgctac
     3481 cattacggta aatgcctacc cggtggcaac gctgacgaat ataccggtaa cgctgtgcct
     3541 gtcagatcct tcttacagcc tgacagcatc aacagcgggt ggaacctgga gtggtgctgg
     3601 tgtaacaggc agtagttttt caccatctgc agcgggactt ggtaccaaga cgatcagtta
     3661 tacgctgaac aatgccggtt gtgtaacgac gaagacatcg atcatccaag tgaatg
//
LOCUS       DAORLS010000295         3706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13579_length_3706_cov_2.148179, whole genome shotgun sequence.
ACCESSION   DAORLS010000295 DAORLS010000000
VERSION     DAORLS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13579_length_3706_cov_2.148179"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PK977_16760"
     CDS             <1..636
                     /locus_tag="PK977_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRF19830.1"
                     /translation="CGPCHAEEVALEKLSYLTFSGVDTNTASELAAHFTTSYINTIVN
                     HDILGVQYAAVLKNIYALGAGIAHGLDYGDNFLSVYIANAADEMAGFLKQFGAEHITV
                     GEHEGEDPVTHRKTPNYAASVYLGDLLVTCYSLFSRNRTFGNMIGKGYSVQSAQLEMN
                     MVAEGHNAARCIYNINQEVNAAIPIAETIYQILWENVKPSDGFKRIEQTLV"
     gene            831..>1022
                     /locus_tag="PK977_16765"
     CDS             831..>1022
                     /locus_tag="PK977_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19831.1"
                     /translation="MIQFFQVVFLIAAVIMIVLNVQTFNLWQESRKEGATKPYPMKWF
                     NFIITSLVAVGCVLNIIVAW"
     assembly_gap    1023..1039
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1139..2248)
                     /gene="hemW"
                     /locus_tag="PK977_16770"
     CDS             complement(1139..2248)
                     /gene="hemW"
                     /locus_tag="PK977_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRF19832.1"
                     /translation="MAGIYIHIPFCRKKCHYCNFHFTTSLYYKDDLVKALANEISLQK
                     DYLQNEKVETIYFGGGTPSLLNSEEVVLILNQVRRYFEVTANPEVTLETNPDDITEEK
                     IIAWKEAGINRLSIGIQSFFEEDLQWMNRAHNAQQAKESLRLAKKHFDNITIDLIYGT
                     PGLTNEKWKQNVETAIELNIPHLSCYALTVEPKTPLDKLIRQKKKEEVNPDKQSEQFL
                     LLMEWMEKAGYEHYEISNFALPGWRSRHNSSYWSGKHYLGIGPSAHSFNGTTRQWNIA
                     NNNLYIESVQAGKVPFEKEQLTSTQQLNEYIMTALRTSEGIDVNKLNKVEADSILQKS
                     KRYLEAALIKQENHHLILTREGKLLADGIAAALFF"
     gene            2336..>3071
                     /locus_tag="PK977_16775"
     CDS             2336..>3071
                     /locus_tag="PK977_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19833.1"
                     /translation="MFILLLAANSLFGQNKYQLIYKGADSLSAATIPKLGLTSHFPSP
                     MNSTEYVYNLVALLQAKGYVSASIDNLQTKGDTTFILLYTGNLYRWAHLSASGVDEKL
                     LDAVGWRPKMFDNKPMDFSQVKTWQERFLSQLENTGYPFAKVYLDSFQLDEDKVTALL
                     KVNKGPLYKIDSLRVYGNAKISNNYLQRYLDIPNGSIYSKEKLLRINKKIRELTYVEE
                     EKPSDLTMLGTGSVLNMYLKQKKSSQV"
     assembly_gap    3072..3099
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3100..>3706
                     /locus_tag="PK977_16780"
     CDS             <3100..>3706
                     /locus_tag="PK977_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF19834.1"
                     /translation="GFLPNNDQLASKKMLITGEGNLNLKNALGSGETIGLNFQALQVE
                     SQRLNFLYQHPYLFRSPFGLDFAFDMFRKDSSFVNVNFQIGAQYVLSTNQAGKVFFQR
                     SSTILNQGGVNGPQVIQTRRLPAAADVSSMAAGVDYEFNNTDYRFNPRKGNDIRVITS
                     VGTKKIKKNNEILDLKDPGDPSFDFDRLYDTVKLKTYQFRFR"
BASE COUNT         1015 a          736 c          782 g         1128 t
ORIGIN      
        1 tgcggtcctt gccatgctga agaggtggct ttggaaaaat tatcttacct caccttttcc
       61 ggagtcgata ctaatacagc ttcagaactg gccgcacatt ttactacatc ttatatcaat
      121 acaatcgtca atcatgacat tttaggagta caatatgcag ctgtgttaaa aaacatttat
      181 gccctgggtg ctggtattgc gcatgggctg gattatggtg ataatttcct gagtgtttat
      241 atcgccaatg cagctgatga aatggcaggg tttctaaagc aatttggcgc tgaacatata
      301 accgttggtg aacatgaagg agaagacccg gtcacccatc gtaaaacacc taattatgca
      361 gcttctgttt atttgggtga tctactggta acctgctact ccctgtttag ccgcaaccgc
      421 acttttggta atatgatcgg caaaggatat tccgttcaat cagcacagct agagatgaat
      481 atggtggcgg aaggtcataa tgcggcccgt tgtatctata atatcaatca ggaagtgaat
      541 gcagcaatac ccattgcgga aacgatttac cagattttat gggaaaatgt taaaccgtct
      601 gatgggttta aacgcataga acagacgctt gtctgagggg taaaacagac cctatgccac
      661 tctcgtacca tggatttgcg ccttcggcta ttttgataat ggttctttgc ctcgttatta
      721 gacaagtgta cctctatggc cggcaacgtt cagtaaaacg ataatatttt aaactcatgt
      781 tttggttcaa agagtaaaat tttatcttca tagttcctaa aaggtatacg atgatccagt
      841 ttttccaggt tgtattttta attgctgcag tgataatgat tgttttaaat gtgcagacat
      901 ttaatttatg gcaagagagc agaaaggaag gcgccaccaa accatacccg atgaagtggt
      961 tcaattttat cattacctct cttgtggccg ttggctgtgt actgaacatt attgtggcat
     1021 ggnnnnnnnn nnnnnnnnnt taaataagcc aatttttttt gccgtataag cactgaaacg
     1081 gagagatttt aagtacctct ttttttagta gtaaattctc tccggcttaa aaaatagttc
     1141 agaaaaataa agctgctgca ataccgtcag ccagtaattt cccttcccgt gttaagataa
     1201 ggtgatggtt ttcctgtttt attaaagcgg cttcaagata tcgtttactt ttttgaagta
     1261 tactatctgc ttcaaccttg ttgagtttgt ttacatcaat gccttctgat gtgcgcaagg
     1321 ctgtcatgat atactcattc agttgctgcg ttgaggtcag ctgttctttt tcaaacggga
     1381 cttttccggc ctggacagat tcgatatata aattgttatt ggcaatattc cattgcctgg
     1441 ttgttccgtt aaatgaatgt gccgaaggac caatacctaa atagtgtttg ccgctccagt
     1501 aagaagagtt atgcctgctt cgccagccgg gcagggcaaa gttggatatt tcgtaatgtt
     1561 cataccccgc tttttccatc cattccatca gcaggagaaa ctgctctgat tgtttatccg
     1621 gattcacctc ttctttcttt ttttgccgta tcagtttatc caatggcgtt tttggttcca
     1681 ctgttaaggc atagcaggag aggtgcggta tatttaactc aatagcagtt tccacatttt
     1741 gtttccattt ttcattggtt aatcccggtg tgccgtaaat caggtcaata gtgatgttgt
     1801 caaaatgctt tttcgccagc cttaagctct cttttgcctg ttgcgcattg tgtgccctgt
     1861 tcatccattg aaggtcttct tcaaaaaaac tttgtatgcc gatactcaag cgattgatgc
     1921 cggcttcttt ccaggcaatt attttttcct ctgtaatatc atctggattt gtttccagtg
     1981 ttacctcagg gttggcagtg acttcgaaat atcgcctcac ctggtttaaa atcaatacca
     2041 cctcttcact attcaatagg gaaggtgtac ctccgccaaa ataaattgtt tcaacctttt
     2101 cattttgcag gtaatctttt tgcaggctta tttcatttgc caatgctttt accaggtcat
     2161 ctttataata aagcgatgta gtgaaatgaa aattgcagta atggcatttt ttgcggcaaa
     2221 agggaatatg gatatagatg cctgccaaag cagtttgtta ttaattgaat aatttagtga
     2281 cttataaaat atccgggcga aaagccagat gctaaaaaca agactgcaaa tatcggtgtt
     2341 catcctgctt ctggcggcca attccctctt tggacaaaac aaataccagt tgatatacaa
     2401 gggagctgat agtttatcgg ctgctacaat acctaagcta ggactgacca gccatttccc
     2461 atctcccatg aacagcactg agtatgtata taatcttgtt gctttattgc aggccaaagg
     2521 gtatgtaagc gcttcgatag acaacctgca aacaaagggg gatactactt ttatactgct
     2581 atataccggc aatctttatc gctgggcaca tttatcagcc agcggtgttg atgaaaagtt
     2641 gttggatgcg gtgggctgga gacctaagat gtttgataat aagccgatgg atttttcgca
     2701 ggtaaaaacc tggcaggaac gtttcctgag ccagcttgaa aataccggtt atccttttgc
     2761 caaagtatac ttagacagtt ttcaactgga tgaagataaa gtgaccgcat tgcttaaagt
     2821 gaacaagggc cctttgtata agattgacag cctccgggtg tatggcaatg caaagatctc
     2881 gaacaattac ctgcaacgat accttgatat tcccaatggc agtatataca gcaaggaaaa
     2941 actgctgcgc atcaataaaa agatccgtga actcacctat gtggaggaag agaaaccgtc
     3001 ggatcttacg atgctgggta ccggctcggt attaaatatg taccttaaac aaaagaagag
     3061 cagccaggtt annnnnnnnn nnnnnnnnnn nnnnnnnnnt ggttttttgc ccaacaatga
     3121 ccaactggca tccaaaaaaa tgctcattac cggtgaggga aacctgaacc tgaaaaacgc
     3181 attaggctcc ggtgaaacca tcggattgaa tttccaggct ttacaggtgg aatcacaacg
     3241 attgaatttc ttgtaccagc atccctactt attccgttct cctttcgggc ttgattttgc
     3301 ctttgatatg tttcgtaagg acagctcttt tgtgaatgta aacttccaga tcggtgcaca
     3361 atatgtatta agcactaatc aggcgggcaa ggttttcttt cagcgatctt ctactattct
     3421 aaaccagggc ggggtaaacg ggccgcaggt gatacaaacc aggcgattgc cggctgcagc
     3481 ggatgtaagt tctatggcgg caggtgtaga ttatgagttc aataatactg attaccggtt
     3541 caacccgaga aaaggaaatg atatcagggt tatcacttct gtgggtacca agaagatcaa
     3601 aaaaaataat gaaatactgg acctgaaaga tcccggcgac cccagctttg attttgaccg
     3661 tttgtatgat acagtaaaat tgaagaccta ccagtttcgc tttcgg
//
LOCUS       DAORLS010000296         3704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13591_length_3704_cov_2.226089, whole genome shotgun sequence.
ACCESSION   DAORLS010000296 DAORLS010000000
VERSION     DAORLS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13591_length_3704_cov_2.226089"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PK977_16785"
     CDS             complement(<1..1032)
                     /locus_tag="PK977_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF19835.1"
                     /translation="MNIAVNTRFLLKDYLEGYGYFIYETFKRITAAPPEHEFIFIFDR
                     PYDERFVFGPNVKAVVTGPPARHPLLWKLWYDIKVPVLLRKYKADVFVSCDGFCSLGT
                     KVPQCLLVHDLAFLHYPSFIPKSHLLYYKRYTPKMLAKAKVIAAVSAFSKKDITSQYG
                     TAADKINIVYSAAKEIFQPLTNEEKQATRAKYTGGKEYFIYAGAIHPRKNLVNLLKAF
                     SLFKKRQQSSMKLVLTGRLAWKYDSFVKNLKTYKYRDDVVMTGYVNEEEIRKLVGAAY
                     TMVYPSLFEGFGVPVLEAMQCDVPVITSLNSSMQEIANEAALYAEPESPADIAEKMML
                     LYKDEKLRSQ"
     gene            1292..>1577
                     /locus_tag="PK977_16790"
     CDS             1292..>1577
                     /locus_tag="PK977_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19836.1"
                     /translation="MKNFYTSVLLLIIQLLSHHFSSAQCPYGFPPGTTAYDTTIATPA
                     GINTLFVKFPQADPYAGMVTCLRLCITITGVVDSVSVENNSASAQTANVYY"
     assembly_gap    1578..1633
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1634..2480
                     /locus_tag="PK977_16795"
     CDS             <1634..2480
                     /locus_tag="PK977_16795"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF19837.1"
                     /translation="SHTYGPYALAATNGILGSGPDFVAISRDTVLNAVTVCTTINNPD
                     SLFQFYGHDSVTYLYNITAFTNVTCTGGNYNSTVATSALVRFRFEYCTCPGYILPLNM
                     TDFRVNKISANKARLTWHDEEYNEVPYHYEPEVSRDGVNFTSLGKLLPQNTGPENRMS
                     FDYASYRQSGTFLFRIKQVYANGFVRFSNIRQVELENSGKPEFILYPNPSNGVVGIKF
                     ANNEGGKFRLELYNTQGQLMMAKEIVATGSSVHQFATLLKGTFWLRVVDVTSQLSCVN
                     QLFIK"
     gene            2614..3297
                     /locus_tag="PK977_16800"
     CDS             2614..3297
                     /locus_tag="PK977_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRF19838.1"
                     /translation="MEYNTTRNHLTMREYGRHVQKMVEHILTIEDPERRQRNAQAVIE
                     LMGFLNPHLKNVEDFRHKLWDHLFLIADFNLDVKSPYPIPTRETLKAKPKPLPYPKRY
                     PKFSHLGKNLEVIIDKALKEENPEKKLGFANAIAYYMKLAYNNWHKEIVHDDAIQSEL
                     TGITQGQLTFTNTPYVKAFRPQQEGRDNRGGYGKQRGKFQQRGGGGGRNDRRDNRGGG
                     GGNFKKKRY"
     gene            3434..>3704
                     /locus_tag="PK977_16805"
     CDS             3434..>3704
                     /locus_tag="PK977_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRF19839.1"
                     /translation="MNSFEVRGGKKLNGAIIPQGAKNEALQIISAVLLTPEEVTISNI
                     PDIIDVNLLIELLGEMNVKVNRISRDTCIFRADDVNIDYLHSEAYK"
BASE COUNT         1051 a          812 c          757 g         1028 t
ORIGIN      
        1 ctggctccgg agtttttcat ctttatacaa cagcatcatt ttctccgcaa tatcagcagg
       61 actttccggt tcagcatata acgctgcctc attagctatc tcctgcatgg agctgttaag
      121 cgaagtaatg acaggcacat cacattgcat ggcttcgagc accggcaccc caaatccctc
      181 gaacaaagaa ggatatacca tggtataagc ggcaccaacg agttttctta tttcttcctc
      241 atttacataa ccagtcatta ccacatcatc ccggtacttg tatgtcttca ggtttttaac
      301 aaacgaatca tatttccagg cgagccggcc agttaaaacc agtttcatcg aagactgctg
      361 tctttttttg aagagggaaa atgctttcag caaattcacc aggttcttcc ttggatgaat
      421 agccccggca tagataaagt attctttgcc ccctgtatat tttgccctgg ttgcctgttt
      481 ttcttcattt gtcagtggtt gaaatatttc tttggcagcg ctgtatacta tattaatctt
      541 gtccgcagct gttccatatt ggctagttat atcttttttg gaaaacgcag atacagcagc
      601 aatcactttt gcttttgcca gcattttggg agtatacctt ttataataca gcaagtgtga
      661 tttaggtatg aaagacggat aatgtaaaaa agcaaggtca tgcaccagca ggcattgcgg
      721 aacttttgtg cccagggaac aaaatccgtc acaggaaaca aatacatcag ccttgtattt
      781 ccgaagcaaa acgggcactt ttatatcata ccataatttc cagagcaatg gatgacgggc
      841 cggcggacct gtgactactg cttttacgtt tgggccaaaa acaaatcgtt catcatacgg
      901 cctgtcaaat ataaatatga actcatgctc agggggtgct gctgttatgc gtttaaatgt
      961 ttcataaatg aaataaccat atccctccag gtaatccttc agtaaaaacc ttgtattgac
     1021 tgcaatgttc attcgtggcg caaagttacc ggaagcggta gtattcctac cgtaaaaaac
     1081 acaataacgt acgagggact gctattaatt tctccgattg aattcattga ttttttctat
     1141 cccctgttga tataaaatac aggaagattt gctgtggata ttcatttaat acggcttaga
     1201 aaacagggca tatctgctct tgtatcacta tgggtatacc tttagtttta catcgctaac
     1261 aattattatc cggtaaccaa aactgtagcc tgtgaaaaac ttctacactt cagttttact
     1321 gctgatcatt caattgctta gccaccattt cagttcagcc caatgccctt acggttttcc
     1381 gccgggcacc accgcctacg ataccactat tgccacacca gcaggtatca acacattgtt
     1441 tgtaaagttt ccgcaagcgg atccttatgc aggtatggtt acctgtttgc gattatgtat
     1501 taccataacc ggtgttgtag actccgtgag tgttgaaaat aattctgctt cagcgcaaac
     1561 ggctaatgtg tattatannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnncagccac acttacggcc cctatgcttt ggctgccaca aacggcatcc
     1681 tgggttcagg acctgatttt gtcgccatat ccagagatac tgtactgaat gctgtcaccg
     1741 tttgcaccac catcaataac cctgattctc tgtttcagtt ttatggtcac gatagcgtta
     1801 cctatttata taatatcacc gcatttacta atgtaacctg caccgggggc aattacaaca
     1861 gtaccgtggc cacttccgct ttggtgaggt ttcgatttga atattgcact tgcccgggct
     1921 atattcttcc gctgaatatg accgatttca gggtaaataa aatttctgct aataaagcaa
     1981 gattgacatg gcatgatgag gagtataatg aagtgcccta tcactatgag ccggaggtca
     2041 gcagggatgg tgtaaatttt acaagcctgg gaaaactgct gccacaaaac accggtccgg
     2101 aaaaccggat gagttttgat tatgcaagct accggcaaag cggaacattc ttgttcagga
     2161 tcaaacaggt atacgccaat ggatttgttc ggtttagtaa tatccggcag gtagaactgg
     2221 aaaattccgg taaaccagaa tttattctct atcccaaccc ttccaatggg gtcgttggta
     2281 tcaaatttgc taataatgag ggcggaaaat tccgactgga attatataac acacagggac
     2341 aattgatgat ggcgaaggaa attgtggcga caggctcttc ggttcatcag tttgccacgt
     2401 tactaaaggg aacattttgg ttaagggttg tggacgtaac gagccaactg tcctgtgtca
     2461 atcaactttt cattaaatga cctcttaggt ggaagaggat ggtggcttgt aaaaggggcc
     2521 ttggttcggg gcccctttct tttaccctct tcacatcaga cttcctgttt cggacttctt
     2581 tctctacatt tgtcgctgca ctaaagcatt ttcatggaat ataatactac aagaaaccac
     2641 ctgactatga gggagtatgg ccgtcatgtg cagaaaatgg tggagcacat tttaacgatc
     2701 gaagaccccg aaagacgcca gcgtaatgca caagctgtta ttgaactaat gggttttctg
     2761 aaccctcacc tgaagaatgt ggaagatttt cgccacaaac tctgggacca tcttttcctg
     2821 atcgctgatt ttaacctgga tgtaaaatct ccttacccta tacctacaag agagacatta
     2881 aaggcaaaac ccaaaccact tccttatccc aaacgctacc ccaagttttc tcacctcggt
     2941 aagaacctgg aagtgatcat tgataaagcg ctgaaagaag aaaatcctga aaagaaatta
     3001 ggtttcgcca atgccattgc ctactacatg aaactggctt acaacaactg gcacaaagaa
     3061 atagtacacg atgatgccat tcaaagtgaa ttgaccggca tcacacaggg ccagcttacc
     3121 tttaccaaca ctccttatgt aaaggcattc cgtccgcagc aggaaggaag ggacaaccgc
     3181 ggcggctacg gcaagcaaag aggaaaattc cagcagcggg gtggcggtgg tggccgcaat
     3241 gacagaagag ataaccgtgg cggtggcggc ggaaatttca agaagaagag gtattaagtt
     3301 tattcgttaa atagttaaat ggttaatcag ttcgctggtt aaccattttt tattggttaa
     3361 gagcctccct ttacagttac tttgcagcag atacaattcc tattacccaa tacctaatta
     3421 actaatcaac tacatgaact ccttcgaagt aagaggtggt aaaaaactaa atggtgctat
     3481 cattccgcaa ggagccaaga acgaggcctt gcaaataatc agtgctgtat tgctgacacc
     3541 cgaagaagtc actatcagta atattcccga tatcattgat gtaaacctgc ttatagaatt
     3601 actcggcgaa atgaatgtga aggtgaaccg catcagccgt gatacctgca tattccgggc
     3661 cgacgatgta aacatcgatt acctgcacag cgaggcttat aaaa
//
LOCUS       DAORLS010000297         3662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13802_length_3662_cov_1.906848, whole genome shotgun sequence.
ACCESSION   DAORLS010000297 DAORLS010000000
VERSION     DAORLS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13802_length_3662_cov_1.906848"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK977_16810"
     CDS             complement(<1..297)
                     /locus_tag="PK977_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19840.1"
                     /translation="MAILKFRVYFEEDESVYRDVVIRHSQTFFELHEAILKSYEFDNK
                     HKATFYRSNDHWQRGREISLELYEKEYKAAPLIMSETTIGSEIRDPNQRFIYQYD"
     gene            complement(307..525)
                     /locus_tag="PK977_16815"
     CDS             complement(307..525)
                     /locus_tag="PK977_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19841.1"
                     /translation="MEEKDHVRNWQPPVDGEMIMEMFGLPPSKPVGILKDSLKDAMLD
                     GIIPNTKEAAVDFVVEKAKSMGLELKKS"
     gene            complement(592..>853)
                     /locus_tag="PK977_16820"
     CDS             complement(592..>853)
                     /locus_tag="PK977_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase"
                     /protein_id="HRF19842.1"
                     /translation="KKYEDGHGWTFHGHEVVGGRMVPKIFAKLKLPQNEKMRFVRKMV
                     ELHLRPISLTKENITDSAIRRLLFDAGDDFDSLMLLCEADIT"
     assembly_gap    854..873
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<874..1739)
                     /locus_tag="PK977_16825"
     CDS             complement(<874..1739)
                     /locus_tag="PK977_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRF19843.1"
                     /translation="MDITCTDKELFILKKVAGAAQELGMETYLVGGFVRDKLLGRDSK
                     DADFVCVGDGIALAQAVAQKMNPVPKVEYFKNFGTAHIRVNDDFDIEFVGARKESYQS
                     HSRKPAVEPGTIADDQDRRDFTINALAIGLNKDNYGKLVDPFDGLKDLKSKIIRTPLE
                     PSQTFSDDPLRMMRGIRFATQLGFTIEETTWQGIIDNAHRIKIISQERITDELNKIIA
                     SVKPSLGFDLLYKSGLLQLIFPQMVELAGAEYKDGVGHKDNFYHTLQVLDNLAAKSDD
                     LWLRWAAILHDIA"
     gene            complement(1784..2353)
                     /locus_tag="PK977_16830"
     CDS             complement(1784..2353)
                     /locus_tag="PK977_16830"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRF19844.1"
                     /translation="MVDFEKDIENCLQVLDNGGLILYPTDTVWGIGCDATSEKAVEKV
                     YQLKNRPDQKAMIVLVADERDVLQYVASPDLAVFDYLQETKKPTTVIYEGAIGLADNL
                     LGKDGSIAIRICNEQFCKHLIKRFRKPIVSTSANISGEPAAKTFADINEQIKSGVDYV
                     VTYRQDDKTIAAPSSVIKWNNGHLEVIRP"
     gene            complement(2347..>2907)
                     /locus_tag="PK977_16835"
     CDS             complement(2347..>2907)
                     /locus_tag="PK977_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF19845.1"
                     /translation="DQFFLYVGSIIERKNLLNVCKALFLLREDLDIPLVVIGEGGKYK
                     QQVKDFVLQNGLEKNVIFLSENEKAKTSPAFRSAAIFPAIYQSAVAMIYPSLFEGFGI
                     PVLEALWSRLPVITSNVSCLPEAGGQGAYYVNPVISEEIAGGMKRIFEDKNLADSLKE
                     KGWQHAQNFTQQKCADAVMEVYKSLW"
     assembly_gap    2908..2955
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2956..3503)
                     /locus_tag="PK977_16840"
     CDS             complement(<2956..3503)
                     /locus_tag="PK977_16840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF19846.1"
                     /translation="MNIGFDAKRAFHNGTGLGHYSRTLIHSLSTFHPEHHYYLFNPRK
                     ANLFTMEGEHLHEVLPSGVINRLFRSAWRSSWVKKDLKRLHIDIYHGLSHEIPVGINK
                     TGIKSVVTIHDLIHERYPEQYNAIDVKIYSQKFRYACKHSDKVIAISEQTKRDIIEFY
                     KTPEEKITVCYQSCNPAFGQIIS"
BASE COUNT          998 a          846 c          668 g         1082 t
ORIGIN      
        1 atcatactgg tatataaacc gctggttcgg atccctgatt tctgaaccaa tcgttgtttc
       61 gctcataata agcggtgccg ctttatattc tttttcgtat agctccagtg atatttcacg
      121 gcccctttgc cagtgatcat tgctgcggta aaaagtagcc ttgtgcttat tatcgaactc
      181 gtatgacttg agtattgctt cgtgcagttc aaagaaagtc tggctgtgcc ggatcactac
      241 atcacggtac acactttcgt cttcttcaaa atataccctg aattttaaaa ttgccatatc
      301 acaaatttaa gacttcttca attccaaacc catgctcttt gccttctcaa caacaaaatc
      361 aacggctgct tctttcgtat tcgggataat accatccagc atggcatcct tcagcgaatc
      421 ttttaatata cccaccggtt tggaaggcgg caacccgaac atttccatga tcatctctcc
      481 atcaacaggc ggctgccagt tacggacatg gtctttttct tccacttctt tacaacgttg
      541 tcttaccagc tgaaagtttt ctaaaaaccg ctttactttc tgtttgttct tttaggtaat
      601 atctgcctcg cacagcagca tcagcgaatc aaaatcatcg ccggcatcaa acaataaacg
      661 gcggatagca gagtccgtga tattctcttt ggtcaggctg atgggccgaa gatgtagttc
      721 taccattttt ctcacaaacc gcattttctc gttttgcggc agctttaatt tagcaaatat
      781 tttgggcacc atacggcctc ccactacttc atgcccgtga aaggtccagc catgcccatc
      841 ttcatatttc ttgnnnnnnn nnnnnnnnnn nnngcaatat catgcagaat agctgcccag
      901 cgcaaccaga gatcatccga ttttgcagca agattatcca ggacctgcaa agtatgatag
      961 aagttgtctt tatgcccgac cccgtcctta tattcagcac cggccagttc caccatttgc
     1021 gggaatatca gttgcagcag gccgctttta tacaacaggt cgaaaccaag ggaaggtttt
     1081 actgatgcaa tgatcttgtt cagttcatct gttattcttt cctgcgaaat gatcttaatc
     1141 cgatgcgcat tatcaataat accctgccag gttgtttctt caatggtgaa acctaattgt
     1201 gtggcaaagc ggataccccg catcatacga agcggatcat cactaaaagt ttgtgatggc
     1261 tccaggggtg tacgaatgat cttgcttttg aggtctttca atccatcaaa aggatcaacc
     1321 aattttccat agttgtcttt attcagccct atggccaatg cattaatggt aaaatcacgg
     1381 cggtcctggt catcggcaat agtacctggc tctacagctg gttttcttga gtgcgactgg
     1441 taactttcct ttctggcacc aacaaactca atatcaaaat catcattaac cctgatatga
     1501 gctgttccga aatttttaaa gtattcaacc ttcggcacag ggttcatttt ttgggctact
     1561 gcctgtgcta atgcaatgcc atctccaaca caaacaaaat cggcgtcttt tgaatccctt
     1621 cccagcagct tatcccgcac aaaaccgccc accaggtagg tctccatgcc cagttcctgg
     1681 gccgctccgg caaccttttt caggataaac aattccttat ccgtgcaagt aatatccata
     1741 ccgggcaaag gtaacagtta gcttctaagg ttaaaaggag tacttaagga cgtatgacct
     1801 caaggtgccc gttgttccat tttataacag acgaaggcgc tgcaatggtt ttatcatcct
     1861 gccggtaagt aactacataa tcaacacctg atttgatctg ctcattaata tctgcaaatg
     1921 ttttagcagc aggctcaccg gaaatattgg ctgaagttga aacaattggt tttctgaaac
     1981 gcttaataag atgtttgcaa aactgttcat tacaaatgcg aatagcaatg cttccatctt
     2041 tgccgagcag gttatcagcc agcccaatag cgccttcata aatcacagtg gtaggtttct
     2101 ttgtctcctg taaataatca aacacagcca aatccgggct ggcgacatat tgcaacacat
     2161 cccgttcatc agctaccaaa acgatcatgg ccttttgatc cggtctattc ttcagttggt
     2221 atactttctc cactgccttt tcactggtag catcacaacc tataccccaa acagtatcgg
     2281 tggggtaaag tatcaagccg ccgttatcca gcacctgcag gcagttctca atatcttttt
     2341 caaagtctac cataaacttt tataaacttc cataacagca tccgcacatt tttgttgggt
     2401 aaaattctgt gcatgttgcc atcctttttc ttttaaagaa tcagctaaat ttttgtcttc
     2461 aaaaatcctt ttcattcccc ctgctatctc ttctgaaatg accggattca cataataagc
     2521 tccttgtccg ccggcttccg gcaaacagga aacattagat gtgataacag gcaaacgact
     2581 ccataacgct tccaaaaccg gtataccaaa tccttcaaaa agggatggat agatcatggc
     2641 aacggcagac tgataaatag ccgggaaaat agcagctgaa cgaaatgcag gggatgtttt
     2701 tgctttttca ttttctgaca aaaagatcac attcttttct agtccgtttt gtaacacaaa
     2761 atcctttacc tgctgtttgt attttccacc ctcaccgatc accaccagcg ggatatcaag
     2821 gtcttcccgt aaaaggaaaa gtgccttgca cacattgagc aaatttttcc tttcgataat
     2881 agagcccaca tacaaaaaga attgatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnngatat aatttgacca aaggcagggt tacaactctg gtagcatact
     3001 gttattttct cttcaggtgt tttataaaat tcaataatat ctctctttgt ttgttcactg
     3061 atcgcaatta ctttatcaga atgcttacag gcataccgga acttttgact gtatattttg
     3121 acatctatgg cattatactg ctcaggatac ctttcatgaa tcaggtcatg aatagtcacc
     3181 accgatttga ttcctgtttt atttattcca actggtattt catggctcaa tccatgatag
     3241 atgtcaatat gaaggcgttt aaggtctttc ttaacccaac tgctccgcca tgctgatcga
     3301 aacagtctgt tgataacacc ggaaggtagt acttcatgaa gatgctcacc ttccattgta
     3361 aataaattag ccttcctggg attaaaaaga taatagtggt gttcggggtg aaatgttgat
     3421 aaagaatgga tcagtgtgcg gctgtaatga cccagcccag ttccgttgtg aaaagccctc
     3481 tttgcatcaa acccgatatt cattccgcaa aagtaacagg gatcagggaa taggcaatga
     3541 tgaatggaga atactaccat atgggtaaaa tattcagcaa cgctattgac cattcactat
     3601 tttcgcagca aatttttgga aaatgaaaga acttatactg gaagcatggg gtaaccgtga
     3661 at
//
LOCUS       DAORLS010000298         3641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13907_length_3641_cov_2.426659, whole genome shotgun sequence.
ACCESSION   DAORLS010000298 DAORLS010000000
VERSION     DAORLS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13907_length_3641_cov_2.426659"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK977_16845"
     CDS             complement(<1..656)
                     /locus_tag="PK977_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRF19847.1"
                     /translation="MVIDFRSDTVTKPTQGMLEAMFKAKVGDDVFGEDPSINELEAMV
                     TEMFGMEAAIFCPSGTMTNQIAIKCHTQPGDEVICDESSHIYQYEGGGIAFNSGASVK
                     LLAGNRGRINVEQVATAIQPDDPHRAHTSMVSLENTSNRGGGSCYDFKEIKKISSLCK
                     EKGLAFHLDGARLWNALIAKKESPKQYGEAFDSISLCLSKSLGCPVGSLLLGKKDFIK
                     "
     gene            1001..3445
                     /locus_tag="PK977_16850"
     CDS             1001..3445
                     /locus_tag="PK977_16850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF19848.1"
                     /translation="MRKLLTIITLITLSFTSRAQISNGKVNGTVIDGSAKIIESATIS
                     LLRVKDSSVAKIAVANRNGQFSFENVAEGSYVVSISAVGHSKGFSETFDISPSNHNVT
                     LKTIELVALAKNLGGVTVTARKPLIEQKIDRTILNVDASATNVGTSAMEVLEKSPGVS
                     VDKDGNISLKGKQGVQVYIDGRPSYLSGTDLANYLRSLSSSQLEIMTNPPAKYDAAGN
                     SGIINIKTKKTKQFGYSGSISSTYSQGRYAKFNESFNFNYRKNKINFFTTLGYNNRKN
                     FQELNIQRKFLESFTKEIISHYDQVSKMRDNGESFNAKLGLDFYTTKKTTLGAVVTGF
                     YNPGTFGNRSDVLISDPNRVLLSRTLASANNERKWKNFSGNLNFRHVFDSTGRELTAD
                     LDYITYNSTNKQDMVNAYFNAGGIPTIKADTLLGNLPQDINIYSAKMDYLHPLKNGAK
                     FEAGVKTSFVRTDNNAVYDSINYGVRVRDIGRSNHFIYEENVNAAYVNFSRPISKKVF
                     GQLGLRVENTNTKGKQITTNQQFERNYTQLFPTAFVQYKINDKHTLGLNYGRRIERPD
                     YEDLNPFILFLDRYTFQQGNPNLKPQFAHNVELTHTFKGFLNTTLNYTNTTDIINDVL
                     EQDANKNETVIRKQNIARQRQYGISVSAGGQVTKWWTGNVYANVFNNLFKGVVNGDNV
                     EIGATAAQFNISNQFKFAKTWSAELGGFYTTGGADGVFRIRDFGMLNMGISKQILKGK
                     GTIRLNVRDMLYTQKIRGDIRYSNIDASFQQRRDSRQVAVGFTYRFNKGKVNAQKRKT
                     GGASEEQNRVKTGGEN"
BASE COUNT         1193 a          685 c          735 g         1028 t
ORIGIN      
        1 tttttgataa agtctttttt gcctaacagc aaacttccga ccgggcatcc caggctttta
       61 ctcaggcaaa gggagatgct gtcaaaggct tctccatatt gtttaggaga ttcttttttg
      121 gcaattaaag cattccacag ccttgcacca tccaggtgaa atgctaagcc cttttccttg
      181 cacagagagc tgattttttt tatttcttta aaatcgtagc aacttccacc acctctgttc
      241 gaagtatttt caagcgagac catacttgta tgagccctgt ggggatcatc cggctgaatg
      301 gctgttgcaa cctgctccac attaattcta cccctattac cagccaataa tttaactgag
      361 gcaccagagt taaaggcgat accaccacct tcatattggt aaatatgaga gctctcgtca
      421 caaataacct catcgccggg ttgagtatga cattttatag caatttgatt ggtcattgtg
      481 ccggatgggc aaaaaatcgc tgcctccata ccaaacattt ctgtcaccat tgcttccagt
      541 tcattgattg aggggtcttc gccaaaaaca tcatcaccca cttttgcttt aaacatagct
      601 tccagcatgc cctgcgtcgg ttttgtaact gtatccgatc tgaagtcaat aaccatttta
      661 aaagttttct gcaagatact aataccgtgt ctaagtcagt ggaaatgagc tgaataatca
      721 gcgaaagagt aattagttga agttaaatgc cgaaaaattg tctctaaggt tggaaaaagg
      781 gcaataccag ccatttaacc gcaaaatcca ccttttatac caagtagtac cagtttctgt
      841 aacccaattt accttgttgc gtcataccct tgtctttcaa aacggccgta catcactaaa
      901 ataggccgtt taatttaaaa caatgtacta tgtaaaacaa agtactgaat tttgaactgt
      961 aataaataaa aacaagtact aacaaataaa gagaacgaaa atgagaaaac tattaaccat
     1021 tatcaccctt attactttgt cctttaccag cagggctcaa atcagcaacg gtaaagtaaa
     1081 tggtaccgtt attgacggaa gtgccaaaat cattgaatca gcgactatat cgcttttacg
     1141 ggtaaaggat tctagtgttg ccaaaattgc tgttgctaac aggaatggcc agttcagttt
     1201 tgaaaatgtt gctgaaggca gctatgtggt ttcaatcagt gcggttggtc acagtaaggg
     1261 cttttcagag actttcgata tttccccatc aaaccataat gtcacattga agaccataga
     1321 actggtggct ttagcaaaaa atttaggcgg agtgactgtt actgccagga aacccctgat
     1381 tgagcaaaaa atagacagaa ctatattgaa tgtggatgct tctgcaacta atgttggaac
     1441 atctgctatg gaggtgttgg agaaatctcc gggagtatcg gttgataagg atggcaatat
     1501 cagtttgaag ggtaaacagg gggtacaggt atatattgat ggccgaccca gttatctttc
     1561 aggtactgac ctggccaatt accttcgtag ccttagcagt agtcagcttg agatcatgac
     1621 caatcctcct gccaaatatg acgctgcagg taatagtggt attattaata tcaagacgaa
     1681 aaaaacaaag cagtttggat attccggcag tatatcttca acctatagcc agggcaggta
     1741 tgccaaattc aacgaatcat tcaattttaa ttatagaaag aataagatca atttcttcac
     1801 gacacttggg tataataaca gaaagaactt ccaggaactg aatattcagc gtaagttttt
     1861 agaatctttt actaaggaaa ttatttcaca ttatgaccag gtttccaaaa tgagagataa
     1921 tggagagtcg ttcaatgcta aactgggact tgatttttat acaaccaaga agactacttt
     1981 aggtgcagtc gtaaccggtt tttataaccc gggaactttt ggtaaccgca gtgatgtttt
     2041 gatctctgat cctaacaggg tgttattaag ccgtacattg gcaagtgcta ataatgaaag
     2101 aaaatggaag aatttcagtg gtaaccttaa ttttcgccat gtgtttgatt caacgggcag
     2161 ggaattgaca gctgatcttg actatatcac atataacagt accaataagc aagacatggt
     2221 caatgcatac tttaatgccg gtggaattcc cactattaaa gcagatacat tgctgggcaa
     2281 tctaccgcag gacattaata tctattcagc caaaatggat tacctgcatc cgctgaagaa
     2341 cggagcaaaa tttgaagcag gtgtaaaaac cagctttgtt agaacagaca ataatgcggt
     2401 gtacgatagc ataaattatg gtgtaagagt cagggatata ggcagaagca atcattttat
     2461 ctatgaagag aatgtaaatg cagcgtatgt aaatttcagc cggccaataa gcaaaaaggt
     2521 attcggacaa ctaggcttaa gggtggagaa tacaaataca aagggtaagc aaattacaac
     2581 caaccaacag tttgaacgta actatactca gttattccct acagcatttg ttcagtataa
     2641 aataaatgat aagcacactt taggactgaa ttacggacgc aggattgaac gtcctgatta
     2701 cgaagacctg aatccattta ttcttttcct tgacaggtac acttttcagc aaggaaaccc
     2761 taacctgaaa ccccagtttg cacacaatgt ggaactgaca cataccttca aaggattttt
     2821 aaatacaaca cttaattata ccaatacaac agatattatt aatgatgtat tagagcagga
     2881 tgccaacaag aatgaaacgg tgatcagaaa gcaaaatatt gcccgtcagc gtcagtatgg
     2941 aatctcagta agtgcaggag gccaggttac aaagtggtgg acagggaatg tgtatgcaaa
     3001 tgtgttcaat aatttattca aaggtgttgt aaacggtgac aatgtagaga taggagcgac
     3061 agcagcccag ttcaacatct caaaccagtt caaattcgcc aaaacatgga gtgcagaatt
     3121 gggtggtttc tatacaaccg gtggtgctga cggtgtcttt agaatcaggg attttggcat
     3181 gttgaatatg ggaatcagta agcagatatt aaaaggtaaa ggaacaattc gtctaaatgt
     3241 ccgtgatatg ctgtataccc aaaagataag aggagatatt cgctatagca atattgatgc
     3301 ttcatttcaa cagcgcaggg attcaaggca ggtggctgta ggatttacat accgttttaa
     3361 taaaggaaaa gtaaatgcac aaaaaagaaa aaccggtggc gccagcgaag aacaaaacag
     3421 ggtaaaaaca gggggtgaaa actaattttc tccattaatc attaaaccta tccattcatc
     3481 agtcacactg gttttcagtg tgactgattt tttatatatg ttaattggtt tttaacatga
     3541 atgataaaaa cactttcaat tttaccgttt gtgtattggt aaataatatt tgccccttgt
     3601 cagtacttga atgaagcttg tcataattca cttcataagc c
//
LOCUS       DAORLS010000299         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13948_length_3633_cov_1.808552, whole genome shotgun sequence.
ACCESSION   DAORLS010000299 DAORLS010000000
VERSION     DAORLS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13948_length_3633_cov_1.808552"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>233
                     /locus_tag="PK977_16855"
     CDS             <1..>233
                     /locus_tag="PK977_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19849.1"
                     /translation="TLNIQEAIWKNDNNSNELVQKEMKLKEKITRLSLKAGNTTDTAE
                     NGKLLASIRDYEIELGNVKEQLNKDPKWLQKKL"
     assembly_gap    234..247
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <248..1404
                     /locus_tag="PK977_16860"
     CDS             <248..1404
                     /locus_tag="PK977_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF19850.1"
                     /translation="EIPTIHQLQKKLDNNTALLSYHLSEGELLTLLITGTKFIYYKYP
                     LRQNFFTDIETFKSALHNVTIEADYTGTIAAMNLYQILIEPLQSDLAHLKRLIIIPDD
                     ELNYLPFEALENEKKQFLAEKYAITYQYTAALFGQKNTAAYSGGTLGFAPFSDAGFHN
                     IGGDNYSILPFSADEINNLKGKSLLSHQARKDSFLVNANKFKIIHLATHASMNNTEPL
                     RSFILFHPSDPDNKLFAQEIYNMNLDSTQLVILSACETGGGQLVKGEGLMSLSRAFAY
                     AGCPNIITSLWKASDKNTAFITSRLHYYMDKGFSRDMALQQAKLDFLNNTDIEPRYKS
                     PVYWANLILIGNYEPYHKNNNWWWIAIVLITGAFIYKMMKGKSLPKTEKA"
     gene            complement(1435..>2010)
                     /locus_tag="PK977_16865"
     CDS             complement(1435..>2010)
                     /locus_tag="PK977_16865"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HRF19851.1"
                     /translation="NILRFRNGFDTLFATGTLPNARLKKIKLILGAQNSVMKNGQSFS
                     LRVKSEDREVIANLDNSLFDITATGQVLFWIDFDAGNSIQVDNSGQGNNNGYRLKSHI
                     KIFSRSHSGQIEGRVLPRAADPIVKAVFGSDTATAIPDHDDGDFKIVGLHPGIYQLII
                     DGQNGYSDTSINNVVVREREDTHLPTFTLRQ"
     assembly_gap    2011..2087
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2088..2277)
                     /locus_tag="PK977_16870"
     CDS             complement(<2088..2277)
                     /locus_tag="PK977_16870"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19852.1"
                     /translation="MKNMIRPAFLLMFIAVTIFYSCQKDSSVSMPDVNKPHLATIYLT
                     DHQTPVFDSVFIDLQRLEV"
     gene            complement(2405..3169)
                     /locus_tag="PK977_16875"
     CDS             complement(2405..3169)
                     /locus_tag="PK977_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19853.1"
                     /translation="MDNSTPSMSEKLVDYLDGKLEPAEKQQMDKLLETDKDLENELEG
                     LLIAREALKQYGLQQKVASVHQQMMNERKAPVRKITSAKRNIRYAIAVAASILLIVAG
                     YIGYNFYTLSSEKVFAANYRTYELTGVRDGSTAESAIETAYKTRKYTELLTIRFDRPF
                     TIKEDFLRGMAYAETGDNANAILSFKKVVEDNKAAHTNVFRDEAEFYLALTYVRNKDF
                     DFALELFKNIESSPEHLYKDRVTSKLIRQVRMLKWR"
     gene            complement(3162..>3633)
                     /locus_tag="PK977_16880"
     CDS             complement(3162..>3633)
                     /locus_tag="PK977_16880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF19854.1"
                     /translation="GSRQDAEDVFQEVMVNFIDLVQKDKFRGESSVKTFLFSLNRFTW
                     LNELKRRGRALAREEKYEKGQERVELDTGHFIAEREGKAEVLRLVGELGETCKKILLL
                     FYYDNLSMKEILEVTDYENEQVVRNKKYKCLKQLEQMINEKPFLRQTLKNLLHG"
BASE COUNT         1093 a          750 c          615 g         1084 t
ORIGIN      
        1 gacattgaat atccaggagg caatttggaa aaatgataat aattccaatg aactggtaca
       61 aaaagaaatg aaattaaaag agaaaatcac acgactctct ttaaaggctg gtaatactac
      121 ggatactgca gaaaacggaa agttgctggc cagtataaga gactatgaaa ttgaactggg
      181 aaacgtaaaa gaacaactga ataaagaccc gaaatggctg caaaaaaagt taannnnnnn
      241 nnnnnnnaag aaattccaac catccatcag ctacaaaaga agctggataa taacacagcc
      301 ttattgtctt atcacctatc agaaggcgaa ctgcttacac ttctgataac agggacaaaa
      361 tttatttatt ataaatatcc gttaagacaa aattttttta ctgatataga aacattcaaa
      421 tctgctttgc ataatgtgac aatagaggca gattatacag gcactattgc agcaatgaat
      481 ctttaccaaa tactcattga accgcttcaa tctgacttag cgcatcttaa acgtttgatt
      541 attattcctg atgatgagct caattatcta ccctttgaag cattagaaaa tgaaaaaaaa
      601 cagtttcttg ctgaaaaata tgccatcaca tatcaatata ctgctgcctt atttggacaa
      661 aaaaatactg ctgcctattc tggaggtaca ttaggtttcg ctccttttag tgatgctggt
      721 tttcataata tagggggaga taattatagc atattgcctt tttcagcaga tgaaataaat
      781 aacctaaaag gcaaaagctt attatctcac caggcaagaa aagacagctt cctggttaat
      841 gccaataaat ttaaaattat acacttggca acacatgcca gtatgaacaa tactgaaccc
      901 ttgcgttcat tcatcttgtt tcatccttct gatcctgaca acaaattgtt tgcacaggaa
      961 atctacaata tgaatcttga ttcaacccaa ctggtgattc ttagtgcctg tgaaacaggt
     1021 ggcggtcagt tggtgaaagg agaaggtctg atgagtttgt cccgtgcttt tgcatacgcc
     1081 ggttgcccca atattatcac atcactttgg aaagcctctg ataaaaatac cgcattcatt
     1141 accagccggc ttcattatta tatggataaa ggctttagca gggatatggc attacagcag
     1201 gcaaaacttg attttctaaa taatactgat atagaaccca ggtacaaatc acccgtatat
     1261 tgggccaatc tcattctaat aggaaattat gaaccctatc acaaaaacaa taattggtgg
     1321 tggattgcta ttgttttaat taccggcgca tttatttaca aaatgatgaa aggcaaaagc
     1381 ctccccaaaa ctgagaaggc ttagttcttc acagatcaat tttttagtcc atatttactg
     1441 gcgaagtgta aaagttggca aatgcgtatc ttctctttcc cttacgacga cattatttat
     1501 tgatgtatcg ctatacccat tttgcccatc aattatcagt tgatatatac ccggatgcag
     1561 gccgactatc ttaaaatcac cgtcgtcatg atcaggaata gccgtagcag tatctgaacc
     1621 aaagacagct ttcactatcg gatcagcagc cctgggaagt actcttcctt ctatttgacc
     1681 tgagtggctt ctgctgaata ttttgatatg tgactttaac cggtaaccgt tgttgttacc
     1741 ctgaccactg ttatcaactt gaatagagtt acctgcatcg aaatctatcc aaaataaaac
     1801 ctggccagtg gcagttatat caaataaact gttgtccagg ttagcaatta cttccctgtc
     1861 ttcactcttc acccgtaatg aaaaggactg gccgttcttc ataacactat tttgtgctcc
     1921 caaaatcaat tttatctttt taagcctagc attgggcagg gtgccggttg caaaaagggt
     1981 atcaaagcca tttcggaaac ggagaatatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta cctcaagccg
     2101 ctgcaggtca ataaagacac tatcaaaaac aggagtctga tggtctgtaa gataaatggt
     2161 tgccaggtga ggcttattga catcaggcat acttactgat gaatccttct gacaagaata
     2221 aaaaatggtg acagctataa acatcagtaa aaaggcagga cgtatcatgt ttttcatata
     2281 acacttttta agtgacagat tgagattgac atgaattagt atccgccaaa catccagctg
     2341 taatcaaaaa aaatcctccc cgggagcagg gaggaaacat aaaccattaa accttaatcc
     2401 ttgattatcg ccatttaagc atccgtacct ggcgaattaa tttagaagta accctgtcct
     2461 tgtataaatg ttctgggctt gattcaatat ttttaaacaa ttccaatgca aaatcaaaat
     2521 ctttatttct cacatacgtt aaggcaaggt agaattctgc ttcgtcacgg aaaacatttg
     2581 tatgggcagc cttattatct tctactactt tcttaaacga aaggatagca ttagcattat
     2641 caccagtttc agcgtatgcc atacccctga gaaaatcttc ctttatagta aatggcctgt
     2701 caaaccgaat tgtcagcaat tctgtatact ttcttgtttt ataggctgtt tcgattgcag
     2761 attccgctgt actgccatcc ctgacaccgg ttagttcata tgttctataa ttggccgcaa
     2821 atactttttc agatgaaagg gtgtaaaagt tgtacccgat ataacctgct acgatcaaca
     2881 aaatactggc ggcaactgca atggcataac gtatgttacg ttttgctgac gttatttttc
     2941 ttaccggtgc tttccgctca ttcatcattt gctggtgaac agaagcaact ttttgctgaa
     3001 gaccatattg ctttaaagct tccctggcaa ttaacaggcc ctccaattca ttttccaggt
     3061 ctttatcggt ctcgagcagt ttgtccatct gctgcttttc agccggctca agtttaccgt
     3121 ccaggtaatc aactagtttt tcagacatac taggtgtaga attatccatg caataagttt
     3181 ttcaaagttt gtcttaaaaa aggtttctca ttgatcattt gttccagttg tttcaggcat
     3241 ttatacttct tgtttcgtac cacctgctcg ttttcataat cggttacctc cagtatctct
     3301 ttcatggaaa gattatcata ataaaagagc agcaaaattt ttttgcaagt ctctcccaac
     3361 tcaccaacca gccttaacac ttctgccttt ccctctcttt cagctatgaa gtggccggta
     3421 tccagttcaa ccctctcctg ccctttctca tacttttctt ccctggccaa tgcccttccc
     3481 cttcttttca gttcattcaa ccaggtaaat ctgttcaggg agaataaaaa agttttgacc
     3541 gatgactccc ccctgaattt atctttctgc accaggtcaa taaaattcac cattacttcc
     3601 tggaacacat cttctgcatc ctggcggctg cca
//
LOCUS       DAORLS010000300         3631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13957_length_3631_cov_1.975951, whole genome shotgun sequence.
ACCESSION   DAORLS010000300 DAORLS010000000
VERSION     DAORLS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13957_length_3631_cov_1.975951"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>372
                     /locus_tag="PK977_16885"
     CDS             <1..>372
                     /locus_tag="PK977_16885"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRF19855.1"
                     /translation="EAAGGLHRFNGWPKPILTDSGGYQVFSLAGTRKIKEEGVTFQSH
                     IDGSKHLFTPETVMDIQRSIGGDIIMAFDECPPGGSEYNYAKKSMEMTHRWLDRCFER
                     FNATEPKYGYTQNLFPIVQGGT"
     assembly_gap    373..482
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <483..924
                     /locus_tag="PK977_16890"
     CDS             <483..924
                     /locus_tag="PK977_16890"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRF19856.1"
                     /translation="MYEICDWCCDHLPVEKPRYLMGVGTPWNILECISMGVDMFDCVM
                     PTRNGRNAMLFTTNGVINIDNKKWEYDFSPIDEGLPCAISNFYSKAYLRHLMKAKEIL
                     GLTIASVHNLAFYLWLVGEARNHMLAGDFQSWKTEMVQRLKQKL"
     gene            988..2085
                     /locus_tag="PK977_16895"
     CDS             988..2085
                     /locus_tag="PK977_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF19857.1"
                     /translation="MKKIDWYILKKFLVTFFFCLLLFTVVAVAVDTSEKTDDFVKANL
                     GTRDIINKYYLGFVPWIWSLLFPLFVFIAVIFFTSRMATRSEVIAILASGTSYNRFLR
                     PYLIGGILLAALLWVGSRYWIPKANTIRSNFQSTYIDKGDPTKNRYFGTCDRCFYKRI
                     DTNSFVGIRDFDTASKTARGFFMEKIAGNKVSYNLRAESMQWDTAVKKWKLINAVERF
                     VDSMGERMKTHTEYTLSLKLEPDDLRKDEYLKDKLTTPELVQFIREEELRGTEGLNSY
                     KVERYRRTATPTAVLLLTLIGAVIASRKTRGGSGMHLALGITIAALFILSDRFSTVFA
                     TKGNFHPMLAAWVPNIVFAGVAWWLYRKTPK"
     gene            2210..3493
                     /locus_tag="PK977_16900"
     CDS             2210..3493
                     /locus_tag="PK977_16900"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HRF19858.1"
                     /translation="MGIIKDRFKAKSEAVAAEIKDILKEHGNKVIGEVTLSQIYQGMR
                     GMTGLVTETSLLDAQEGIRFRGYSIPELQQKLPKAPGGDEPLPEGLFYLMLIGELPTE
                     DEANHVTNILQRRSHVPNHVFEAIDALPLNTHPMTMFVVGVMALQTESYFHKKYAEGI
                     NKKDYWLPVFDDSIVLLSRLPRIAAYIYRRKYKNSDHIQPNGLLDWAGNFAHMLGFED
                     ESFKELMRLYMTIHADHEGGNVSAHTTHLVGSALSDPYLSYAAGMNGLAGPLHGLANQ
                     EVIKWIFEMQEELKTDLPTKEQIADYVKKTLSEGKVVPGYGHAVLRKTDPRFTAQMEF
                     GKKHMPDDKLVQTVWNIYETVPPILQTIGKIKNPWPNVDAHSGALLVHYGMVEYEFYT
                     VLFAVSRALGVLASLCWDRALGFPLARPNLLLQTW"
BASE COUNT          993 a          713 c          846 g          969 t
ORIGIN      
        1 agaggctgca ggcgggctgc atcgtttcaa cggatggccc aaacccatac ttacagacag
       61 tggaggctac caggtttttt cactggcggg tacccgaaaa ataaaagagg aaggggttac
      121 ttttcaatca catattgacg gcagcaaaca cctgttcaca ccggaaacgg taatggatat
      181 acagcgttct ataggcggcg atatcatcat ggcctttgat gagtgtcctc ccggtggcag
      241 tgaatataat tatgccaaga aatcgatgga gatgacacac cgctggcttg acaggtgttt
      301 tgaacgattt aatgctacag aacccaaata tgggtatacc caaaatcttt ttcctatagt
      361 acaaggtggt acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nngatgtatg aaatatgcga ctggtgctgc gatcatttac ctgttgaaaa accccgctac
      541 ctgatgggag taggtacccc ctggaacata ctggaatgta tcagcatggg agtggatatg
      601 tttgattgtg tgatgcccac ccgtaatggt cgtaatgcta tgctgtttac caccaacggg
      661 gtcatcaata ttgataataa aaagtgggag tatgattttt ctccaattga tgagggcctg
      721 ccttgtgcca tcagtaactt ttacagtaag gcctatctcc gtcacctgat gaaggcaaaa
      781 gaaatactgg gacttaccat tgccagtgtt cataatcttg ccttttacct gtggttggtg
      841 ggtgaggcaa ggaatcatat gctcgcaggt gattttcaga gctggaaaac tgaaatggtt
      901 cagcgattaa agcagaaact atagggccgg atcctccaat gctttcacat accttcaaca
      961 gtttttcgcc tacatttgtc cctctcgatg aaaaaaattg actggtacat actgaaaaaa
     1021 tttctggtta cttttttctt ttgcctgctg ctgtttaccg tggtggcagt ggctgttgat
     1081 accagtgaaa aaaccgacga ttttgtaaaa gccaatcttg gcactcggga catcattaat
     1141 aaatattacc tggggtttgt gccctggata tggagtctgc ttttcccatt atttgtgttt
     1201 attgccgtta ttttcttcac cagccgtatg gctacccgtt cagaagtaat tgccattctg
     1261 gcaagtggta ccagctacaa ccgttttttg cggccctatt taattggcgg aatattgttg
     1321 gcagcgttac tttgggtggg tagccgatac tggataccca aggccaatac catccgaagt
     1381 aattttcaga gtacatacat tgataaaggt gatcctacca agaaccggta ttttggcacc
     1441 tgcgaccgat gtttttataa acgaattgat accaattcct ttgttggtat cagggatttt
     1501 gatacagcat ccaaaacagc aaggggtttt ttcatggaga aaattgccgg caacaaggtt
     1561 agttataatc tgcgggctga atccatgcaa tgggacacgg cggtaaaaaa atggaagctc
     1621 atcaatgcag tggaaagatt tgttgacagt atgggcgaaa gaatgaaaac tcatacagag
     1681 tacactctca gccttaaact ggaaccggat gatttacgaa aagacgaata tcttaaagac
     1741 aaactcacca cacctgaact cgttcagttt atcagggaag aagaactacg gggtacggaa
     1801 ggcttgaatt catacaaagt agaacgatac cgcagaacgg caacacccac tgccgtatta
     1861 ctacttactt taataggtgc cgtaatagcc agcagaaaga cccgaggtgg aagtggtatg
     1921 caccttgcac tcggtattac cattgcggct ttgtttatac tctcagacag attttccacc
     1981 gtttttgcca ctaaaggaaa ttttcacccg atgctggcgg cctgggtgcc taatattgtt
     2041 tttgccgggg tggcctggtg gttgtataga aagaccccca aataggactg attaatatca
     2101 ttttctcagc gtataaactt tagtctttaa attttgttgt agcaggtggc attgtgtagc
     2161 tttgccactt caattttttt gattgttttc cccctgaaaa tctgacgtta tgggtataat
     2221 taaagaccgg ttcaaagcta aatctgaagc tgttgccgcc gaaatcaaag acattttaaa
     2281 agaacatgga aacaaagtca taggcgaagt taccctgtcc cagatatacc agggtatgag
     2341 gggaatgacc gggcttgtta cagaaacttc gctgctcgat gcacaggaag gaatccgttt
     2401 caggggatat tcaattccgg agctacaaca aaaattaccc aaagcacccg gcggtgatga
     2461 acccctgcct gaaggattat tttacctgat gcttataggc gaattaccaa cggaagacga
     2521 ggccaatcat gttaccaata ttttacaaag aagaagccat gtgcccaatc atgtatttga
     2581 agccattgat gcgttgccat tgaatactca tccgatgacc atgtttgtgg tgggtgtgat
     2641 ggcgctgcag acggaaagct attttcataa aaagtatgcg gagggtataa ataaaaagga
     2701 ttattggctg ccggtttttg atgacagcat cgtattgcta tcccgtctgc caaggatagc
     2761 tgcttacatt tatcgccgca aatacaagaa cagtgatcat attcaaccca atggtttgct
     2821 ggactgggcg ggcaattttg cccatatgct tggttttgag gatgagagct tcaaagaact
     2881 gatgcgcctg tatatgacca tacacgccga tcatgaaggc ggaaacgtat cggcgcacac
     2941 gactcatctt gttggttctg ccctcagcga tccttatttg agctatgctg ccggtatgaa
     3001 cggccttgcc ggtccattac atggtttggc gaatcaggaa gtgatcaagt ggatatttga
     3061 aatgcaggag gagttgaaga cagatctgcc aacaaaagaa cagattgctg attatgtaaa
     3121 gaaaacattg agcgaaggaa aggtggtgcc gggatacggc catgctgtat tgcgcaaaac
     3181 agatcctcgt ttcactgcac agatggaatt tggcaaaaag cacatgccgg atgataagtt
     3241 agttcaaacc gtatggaata tttatgaaac ggtaccgcca attctgcaaa ccattggtaa
     3301 gatcaaaaac ccgtggccca atgtggatgc acatagtggc gctttgttgg ttcattatgg
     3361 catggtagaa tatgaatttt atactgtgct gtttgccgta agccgtgcat tgggagtatt
     3421 ggccagtctc tgctgggaca gggctcttgg cttcccgctt gcacggccaa atctgttact
     3481 acagacctgg tgaaactgtg gctggatgga aaagataata tctggggaga ataaaattga
     3541 tagttagaaa tgaaaagacc tggataatcc aggtcttttt tgtggaggtt accggagtcg
     3601 aaccggtgac cttttgcatg ccatgcaaac g
//
LOCUS       DAORLS010000301         3630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_13963_length_3630_cov_2.120559, whole genome shotgun sequence.
ACCESSION   DAORLS010000301 DAORLS010000000
VERSION     DAORLS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13963_length_3630_cov_2.120559"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK977_16905"
     CDS             <1..218
                     /locus_tag="PK977_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19859.1"
                     /translation="NALTREEAIRGMTIWAAKSNFEEMEKGSLEKGKFADFVILDRDL
                     MKAADNDILQTVVLKTYLGGEKVYVAK"
     assembly_gap    374..390
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <391..1162
                     /locus_tag="PK977_16910"
     CDS             <391..1162
                     /locus_tag="PK977_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HRF19860.1"
                     /translation="MRCIRTILKEWGEANDLDLHTDEFDVRLQNKVPDTSYDIYISTG
                     GPGDPLITRFDDWDISWCRWLNEMERWNNNPDNIQKKYIFFICHSFQLACRYFNAGLV
                     CKRKSTSFGVFPVHMLVDGRNEEVFHGLKDPFYAVDSRDFQVIQPNIELLEEMGASLL
                     CIEKNRPHVPLERAVMAIRFNDYMIGTQFHPEADALGMLMYLKTEEKRKTVIENHGEA
                     KLNSMIEHLEDPDKIVWTHSHVLPNFLNSAIPVEEMAI"
     gene            1334..1693
                     /locus_tag="PK977_16915"
     CDS             1334..1693
                     /locus_tag="PK977_16915"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRF19861.1"
                     /translation="MKLIPLLLLATSCGSASHQQVSTDTIKVKVKESFEIKLGTNMGT
                     GYSWSLKDSAYGQYMSLDTMYTISNPEGKDNAPETQVFRFTAMKKSNTTLHFLHARPW
                     KKEEKPDQEKQYRVIIE"
     gene            1726..2796
                     /locus_tag="PK977_16920"
     CDS             1726..2796
                     /locus_tag="PK977_16920"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF19862.1"
                     /translation="MKHIKKLPGILLGLALLGCQFGMAQPIQLSEKYKQRELKAPENV
                     RTLLLNQRKQITEQKLQFNVSFTGVSSKTMAQITGEKEAPEQEVNRIKQYIVTRKLTP
                     AAIDITKIRLLGCIASSKKYDARTQGYVTPVRDQRCGNCWAYSALGAYEGSYKRINGS
                     FIDASEQYAENCVDGDCSGGLAYKVMEWMVDNNKNLEKETVAPDAGVNQPCAGGTPTT
                     NYYATDWGVVDPSGDISKIASVASIKDAICKYGPIAASVQVTSLFQNYAGGVFYEFAS
                     NYGSPSSNHAITIVGWDDDKGAWLIKNSWGTDWGEDGYMWIKYNSNNIGRRAAWIIAK
                     KATLTVKPIKDVKIQNKPVIQQ"
     assembly_gap    3039..3260
                     /estimated_length=222
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3261..>3630
                     /locus_tag="PK977_16925"
     CDS             <3261..>3630
                     /locus_tag="PK977_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19863.1"
                     /translation="GFPSLFAYEVLIDDVFRRNYKAPDGFSAYLNGHNRESYLRLLKE
                     IIVGHHQPENVILLEIFPRQQKTRIDFYCTEEYTGIKMVCLTELIKEGKKLFYLNEGK
                     KTEVKRIYNRVIFDDLQQQTA"
BASE COUNT         1078 a          709 c          746 g          858 t
ORIGIN      
        1 aaaatgcact gacaagagaa gaagccatcc ggggcatgac gatatgggct gctaaatcca
       61 attttgaaga aatggaaaaa ggaagtttag aaaaaggaaa gtttgccgac tttgttattc
      121 ttgaccgtga cctgatgaaa gctgctgata atgatatttt gcaaacggtt gtgctaaaga
      181 cttacctggg tggcgaaaaa gtatatgtag ccaaataact acagtagatt aactgaaatc
      241 ataaaatcca catttgtggg taattgttga aacaaacagg cattcatagt cgttattttt
      301 tacctatgtt tgccgctatg catggtacgg aaaaaaggtc tgtaagggta gcaatcctcg
      361 atctctatga aggnnnnnnn nnnnnnnnnn aatgcgttgt atccgaacca tcctgaaaga
      421 atggggcgag gctaatgatc tggacctgca taccgatgag tttgatgtcc gcctccagaa
      481 caaagttcct gataccagtt atgacattta catctccacc ggtggtcccg gtgatccgct
      541 gatcacccgt tttgatgact gggatatcag ctggtgccgc tggctgaatg aaatggaacg
      601 ctggaacaat aaccctgata atattcagaa gaaatacatc ttttttattt gtcactcctt
      661 tcaactggcc tgtcgctatt tcaatgcagg gctggtatgc aaaagaaaat caacttcatt
      721 tggtgttttc cctgtgcaca tgctggtgga tggaaggaac gaagaagtct ttcatggact
      781 gaaagatcct ttttatgctg ttgacagccg cgacttccag gtaatacaac ccaatattga
      841 actgctggaa gaaatgggcg ccagccttct atgtattgaa aagaaccgcc ctcatgtgcc
      901 gctggagaga gcggtgatgg ccattcgttt caatgattat atgatcggca cacagtttca
      961 cccggaagct gatgcactgg gtatgctgat gtacctgaag acagaagaaa aaagaaaaac
     1021 cgtaatagaa aaccacggtg aagccaagct caacagcatg atcgaacacc tggaagaccc
     1081 cgataagatc gtatggaccc actcccatgt attgcccaac tttctgaact ctgccatccc
     1141 ggtagaagaa atggctatct gattctcaat gtgctaaaat aattttaagg ggtactgcac
     1201 ttttcgcctt cttactatat taacttgcat acagagtgtt tcaccaacta tccttccatc
     1261 cacccctctt tgccgactga aacactcggt aagggcaact ggttgcttat ttactgattt
     1321 aaaatctaaa cagatgaaac tgattcctct gttactgctg gctacatcct gcggatctgc
     1381 ttcgcatcag caggttagta ctgataccat caaagtaaaa gtgaaggaaa gttttgaaat
     1441 caagctcggt accaatatgg gaaccggtta tagctggtca ttgaaagatt cggcctacgg
     1501 gcagtatatg tcacttgata ctatgtatac gatcagtaat ccggaaggaa aagataatgc
     1561 gcctgaaaca caggtattcc gttttactgc aatgaagaaa agtaacacta cccttcactt
     1621 cctgcatgcc aggccatgga aaaaagaaga aaaacccgac caggagaaac aataccgggt
     1681 gatcatagaa taaaaaccat ttattaaacc ttaaaacatc catttatgaa acacatcaaa
     1741 aaactaccag gaatactact ggggcttgca ctgctgggct gccagtttgg tatggcacag
     1801 cccatccagt tatcggaaaa atataaacaa agggaattaa aagcaccaga gaatgtcagg
     1861 acactgcttc tgaatcagcg taagcaaatc actgaacaga agttacagtt taatgtaagc
     1921 tttacaggag tcagcagtaa aacaatggcc cagataaccg gtgaaaaaga agcacccgag
     1981 caggaagtaa accgcatcaa acaatatatc gttacccgga aattaacacc tgctgccatt
     2041 gatattacta aaatcagatt actgggttgt attgccagtt ctaaaaagta tgatgcaaga
     2101 acacagggct atgttactcc tgtaagagac cagagatgcg gcaattgctg ggcttattct
     2161 gctttaggag cttatgaagg cagttacaag agaataaacg gcagctttat tgacgcttct
     2221 gagcaatatg cagagaactg cgtagacggc gactgcagcg gaggtttagc ttacaaggta
     2281 atggaatgga tggtagacaa taacaagaac ctggaaaaag aaaccgttgc gcctgatgcc
     2341 ggtgtgaacc agccttgtgc aggcggtaca cctaccacca attactacgc cactgactgg
     2401 ggtgtagttg atccttccgg agatatcagc aaaattgctt ccgtggcttc tattaaagat
     2461 gctatttgca aatacggccc catcgctgcc agtgtgcagg taacaagcct gtttcagaat
     2521 tatgccggtg gtgttttcta tgaatttgcg agtaattatg gaagcccttc ttccaaccat
     2581 gctatcacca tcgttggatg ggatgatgac aaaggtgcct ggctgatcaa aaattcatgg
     2641 gggaccgact ggggtgaaga cggatatatg tggatcaaat ataacagtaa caatatcgga
     2701 cgccgtgcgg cctggatcat tgccaaaaag gcaacattaa ctgttaaacc aatcaaagat
     2761 gtgaagatcc agaacaaacc tgttattcaa cagtaacagt ttatgattga agagaaaagc
     2821 acagccggag ggttgtgctt ttttattgca ggcacctatt cttgtcataa aatgctaatt
     2881 ttatccatac ccaaacaaac caaccatgat cgcatcactg agaaaatctt tcaatgataa
     2941 tttcactaaa gaacaatatg aaaaattcct gaacgacctg cacagcgtac atcccggcca
     3001 ggtagatttt cgggtggctg agactccttt attcgttcnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn gggcttccct tccctattcg cttacgaagt tttaatagat
     3301 gatgttttca gaagaaatta caaagcaccg gatggtttca gcgcttacct gaacgggcat
     3361 aacagggaaa gctatctcag gctgctcaaa gaaatcattg tcggccatca ccagccggag
     3421 aatgtgatcc tgctggaaat atttccccgc cagcaaaaaa caagaattga tttttactgt
     3481 acagaagagt ataccggcat caaaatggtt tgccttactg aattaatcaa agaaggaaaa
     3541 aaacttttct acctgaacga gggtaagaaa accgaagtaa agagaatcta caaccgggtg
     3601 atatttgatg acctgcaaca gcaaacagcc
//
LOCUS       DAORLS010000302         3613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14042_length_3613_cov_1.994660, whole genome shotgun sequence.
ACCESSION   DAORLS010000302 DAORLS010000000
VERSION     DAORLS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14042_length_3613_cov_1.994660"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1109)
                     /locus_tag="PK977_16930"
     CDS             complement(<1..>1109)
                     /locus_tag="PK977_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19864.1"
                     /translation="KDTSGKGGIDIVSSFKPVLRQTAKINFSASPAPVDTTKARLKYE
                     IPTQNLLFAYQPGTLKPLALQIDSAGKFNNSSYIKAGFGSLRTPYIQAGISFGDGKTA
                     GLNIYAKHVASEGKREFQKFSNTQVKLNGYFKSGNNLEWNAGIGMRQDRTYKYGFEPE
                     SLVFPMDSIKQNFQTISGRLSFRNINKTEFGLTYAPEVKIDVFNDNLKNAESNTVVNL
                     PLEKSIGKVFAINLGVTFDLTRLSPKNKTAINNTMYYISPSVLFKTPNINAQVGIRPS
                     WDNKTFKMFPNILADISTEDKRFTFQAGWTGYIRKTTYQYLAGQNPWLFVPGELKNTW
                     IEERFAGFKGSVGDHFTYAAKVGFNRLNNQPLFIN"
     assembly_gap    1110..1133
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1203..>3613)
                     /locus_tag="PK977_16935"
     CDS             complement(1203..>3613)
                     /locus_tag="PK977_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF19865.1"
                     /translation="NYSSVVPYYIAQIYYIQGKKDEAITYIQNKLQGGASSQYYDLEL
                     KQLIGHAYFEKKDYAKALPYLEDYVGRSKKVRREDLYELSYCYYQAKNYTKSIDGFKQ
                     LSSKDDSLSQHAMYLLGDAYLKTGQKSNARNAFLFCSANSSNPEQKEISKYQYAKLSY
                     ELGYQDEALNSLSSFIADYPNSNYNAEAKELMIDVLANTNNYKDAQAMLETLKNPSEN
                     SKRVYPRILLGRAAEMINDGRMDEADQLLTKAISDPYNRTVLPFAYFWKGEIGYRNNQ
                     IDTAIKYFHAYLDAGAPVSGEANPVNARYNLGYAYLRKENYKVSVTFFEPLIKAPALN
                     TDAFSQDAYMRTADNYFMMKSYDKAKLMYDNAIKLSWPSEDYATFQIAMLAGVKSADS
                     KISGMNNMIRKFPKSPLVVDANMEIANTYMGNEKFREAIPYLKVVAENNENTSLVPQA
                     YLKLGTAYYNIDNNDEALRQFKAIVERFPSSSEAEDALDNIKSIYVEQGKPDEYAAYM
                     RQIGKPLSTNTEDELTWAAAEKLYDDQKVNEALNAYNSYLQKFPNGAHVVEANFNRAE
                     IYNIKKDWNNAVPAYEAVAAQAPNQFAERSVLAAARINFFELKNYARAEAFYAQLKQI
                     TSSQELKLEAMRGLLRCQYNQQKWAEASENAKELTAAKGSSADDKTLSNMVIAKSYQV
                     AGQYNEAINSFKSVVQLNKAALAAEARYEIANCWFLLNKLTESEKAAFETINKSGSYD
                     YWITKAYILLGDIYFKQKDYFNAKATFQSVVENTIDPELKEEAQVKLNRATEEEGKSS
                     KVEN"
BASE COUNT          942 a          801 c          653 g         1193 t
ORIGIN      
        1 tcattgatga acaaaggctg gttatttagc ctgttaaaac caactttcgc cgcataagtg
       61 aagtgatctc cgaccgagcc cttaaaacca gcgaatcttt cttctatcca tgtgttcttc
      121 agctcacccg gcacaaacaa ccagggattt tgtcctgcta aatactgata agtggttttc
      181 ctgatatatc ctgtccagcc ggcctggaat gtaaatcgtt tatcttctgt gctgatatcg
      241 gccagtatat tcggaaacat cttaaaagtc ttgttatccc atgaaggtct tatgccaacc
      301 tgagcattaa tatttggtgt tttaaacaat acagagggtg atatataata cattgtattg
      361 ttgatcgccg tcttgttctt gggtgaaagg cgggtaagat caaacgtaac ccccaggttt
      421 atagcaaaca cttttccaat tgatttctcc aaaggcaaat tgaccacagt attgctctca
      481 gcattcttaa ggttatcgtt gaatacatca atttttactt ctggcgcata agtaagtcca
      541 aactctgttt tattgatatt tctaaaggaa agccttccgg atatagtctg aaagttttgt
      601 ttaatagaat ccatcggaaa aaccagcgac tcaggttcaa atccatattt gtaagttctg
      661 tcctgcctca ttccaatacc tgcattccat tcaagattat tgcctgactt aaaataaccg
      721 ttcagcttta cctgtgtgtt actgaacttt tgaaactctc tcttgccttc agatgctaca
      781 tgttttgcat aaatatttaa acctgcggtt ttaccgtcgc caaaagatat acctgcctgg
      841 atatagggag tacgtaaact tccaaaacct gctttgatat aactgctgtt attaaatttt
      901 cctgcagaat ctatttgcag cgccagtggc tttaacgtac cgggctggta agcaaatagc
      961 aaattctgtg tcggaatttc atacttcaac cgggctttag ttgtatcaac aggcgcagga
     1021 gatgcactga aatttatctt ggccgtctgc ctgagtaccg gcttaaaact tgaaacaata
     1081 tcaataccgc cttttccgga agtatccttn nnnnnnnnnn nnnnnnnnnn nnncattgag
     1141 aaaagtaatc ccaacaatat gatacagtat ttattcacgg ttgctttcat tgtctatact
     1201 tcttaatttt ctaccttact tgatttccct tcctcttcag ttgccctgtt taatttcacc
     1261 tgtgcttcct ccttcaattc tgggtcaata gtattctcca caacactctg gaaagtagcc
     1321 ttggcattga aataatcctt ttgtttaaaa taaatatccc caagcaggat atatgctttt
     1381 gttatccagt aatcatagga tcctgattta ttaattgttt caaaagcggc tttttctgat
     1441 tcggtaagtt tatttagcag aaaccagcaa ttggctattt cataccttgc ttcagctgcc
     1501 agtgctgctt tattcaactg tacaacggat ttgaagctgt taatggcttc gttgtattgc
     1561 cccgccacct ggtaagattt tgcaatcacc atattgctaa gggtcttgtc gtcagcactg
     1621 ctaccctttg cagctgtcag ttcttttgca ttttcagaag cctccgccca tttctgctga
     1681 ttatactggc agcgcaataa tccacgcatc gcttccagtt ttagttcctg gcttgaagtg
     1741 atctgcttca gctgagcata aaatgcttcc gcccgggcat agtttttcaa ctcaaaaaag
     1801 ttgatccttg cagcagccag cacagatctt tctgcaaact ggttgggagc ttgtgctgct
     1861 actgcttcat aggctggtac ggcattgttc cagtctttct ttatgttgta tatctccgcc
     1921 ctgttaaaat tagcttccac tacatgggct ccattcggaa atttctgcaa gtagctatta
     1981 taggcattca gcgcctcatt tactttttgg tcgtcataca atttttccgc ggcagcccat
     2041 gttaattcat cttctgtatt agtacttaaa ggtttcccta tctgccgcat ataagcagca
     2101 tattcatccg gtttaccctg ctctacataa atactcttta tgttatccaa tgcatcttcc
     2161 gcctctgatg aactgggaaa tctttctacg attgctttaa actgtctcag tgcttcatca
     2221 ttattatcaa tattgtaata agctgttccc agtttcaggt aagcctgtgg caccaggctg
     2281 gtattttcat tattttctgc tacaactttg agataaggta tagcctccct gaatttttca
     2341 ttacccatat aagtattggc aatttccata ttggcatcaa ccacaagagg agatttagga
     2401 aacttcctga tcatattatt cataccacta atcttactat cagcactttt aacaccagcc
     2461 agcatcgcaa tctggaatgt agcgtagtct tcagacggcc atgatagctt tatagcatta
     2521 tcatacatca actttgcctt atcatagctc ttcatcataa aatagttatc tgctgtacgc
     2581 atataagcat cttgtgaaaa agcatctgta tttaaagccg gcgcttttat caatggctca
     2641 aaaaaagtaa cggagacctt ataattttct tttctgagat aagcataacc aaggttatac
     2701 ctggcattca ccgggtttgc ttcacctgat acaggcgccc ctgcatccag gtaagcatga
     2761 aaatacttga tagcggtatc aatttgatta ttacgataac ctatctcccc tttccagaaa
     2821 taggcaaaag gaagtactgt cctgttgtag gggtcgctga tcgccttggt gagcagctga
     2881 tcagcttcat ccatcctgcc atcattgatc atttctgcag cccgtcctaa taaaatccgg
     2941 ggataaaccc ttttagaatt ttcggaagga tttttcaggg tctccagcat tgcctgtgca
     3001 tccttgtaat tatttgtatt agccagtaca tcgatcatca gttcttttgc ctcagcatta
     3061 tagtttgaat taggataatc agcaataaaa gaactgaggc tattaagggc ttcatcctgg
     3121 tagcctaact catatgacag ctttgcatac tggtatttgg aaatctcctt ctgttctggg
     3181 ttactgctgt tggctgagca aaacaaaaat gcattacggg cattggactt ctggccggtt
     3241 ttcaggtagg catcacccag caggtacatg gcatgctggc tgagcgaatc atctttacta
     3301 ctgagttgtt taaacccatc tatcgatttg gtatagttct tcgcctggta ataacaataa
     3361 gaaagctcat acagatcctc tcttctcacc tttttggaac gtcccacata gtcttccagg
     3421 tagggcaatg ctttcgcata gtcctttttc tcaaaatagg catgaccgat gagctgtttc
     3481 aattcgagat cataatactg tgaactggcg ccgccctgta acttattctg gatataagtt
     3541 atggcttcat ctttcttccc ttgtatatag tatatctggg caatataata gggtacaaca
     3601 gagctgtaat ttt
//
LOCUS       DAORLS010000303         3607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14071_length_3607_cov_2.028435, whole genome shotgun sequence.
ACCESSION   DAORLS010000303 DAORLS010000000
VERSION     DAORLS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14071_length_3607_cov_2.028435"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>260)
                     /locus_tag="PK977_16940"
     CDS             complement(<1..>260)
                     /locus_tag="PK977_16940"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF19866.1"
                     /translation="EFEVPEFYRRLLALLIDMVIQFFYLRIANEIYKSIAMNSSWDID
                     SNYNMQAVGLLLFLPILLYHPLQEITMNGQSVGKKILGIRVV"
     assembly_gap    261..321
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            369..1388
                     /locus_tag="PK977_16945"
     CDS             369..1388
                     /locus_tag="PK977_16945"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRF19867.1"
                     /translation="MFFVFLKAVFYNRIFISLREALFIKKNKDRWLRNQHMPSDNPDE
                     MAADFTQLVDDLAYAKTFYPSGKVTTYINSQASKIYLNIYKNRKEESNRLVTFWKYDL
                     PLTIRKHHRVVFFSFVFFLVFFAIGYFVSLKDEGIPRSIFGDDYVDHTKENIANGNPF
                     GIYEHGNPVLSWLSLMIHNIRVSFLMFVSGIFCGIPSLYMEASNAVMLGSFDQFFTER
                     GLGVDFWLVVFVHGTLEITALIIAAAAGLVLGKSFLFPGTIRRIDAFKQGAKDGVKIM
                     VGLIPVFGLAAFFEGFITRLYNDISALTTLIFALSVVFVVWYFIIYPIRLSKKLSLQL
                     QEEEV"
     gene            1392..>1999
                     /locus_tag="PK977_16950"
     CDS             1392..>1999
                     /locus_tag="PK977_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19868.1"
                     /translation="MKPISTYIRIIFCYRIIVGIFLFTASSSILAQSADEMDTSVIEE
                     VVPADEYSDDEEKSADDEYFWEKEYQDDSWKQHKQRKVSDSAMKKMQEDDDFWYANAV
                     FNKENKKDKNQGGYIPLGQRQWFQTLIWIIIVGGFVTFLIMYLAGTEVGLFRKKSVPV
                     KSREEEEEMPEDIFAINYQKEIDKAAAQGNYRLAVRLMYLRLL"
     assembly_gap    2000..2066
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2226..3479
                     /locus_tag="PK977_16955"
     CDS             2226..3479
                     /locus_tag="PK977_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19869.1"
                     /translation="MKSKLPYIILGLVAVGILMLVATGNYKKNRHFDGRITLRKSDKI
                     PYGSYVAYQNLKSIFPGATIYTDRHEPGYWDSLSNYEKKQAVIILAGRFAADEFEMKQ
                     LIKFAEKGNDVFVIAMEISATAERMLGCDARNYYLASFFESGENPGKDTLTLQLKDVD
                     NQRITQYRYPGRSFNGYFNEVDTVTTHVLGTDAVGRANFIHLQAGDGNFYINMAPVAF
                     SNYFLLHGQNIGYYEKALSYIRPDVQKIVWDEYYLNKKSGGQDNEKKKGWFTVLMNLK
                     NEEGKKPFRAAFWLAILLLFLYVLMEMRRKQRYIPVVAKPRNDSMDFVKTIGRLYYDK
                     GDHRNLCLKMSSYFLEYVRNKYKLPTSKLDEEFIMNLRYKSGVEEPVVRSIISFIKYA
                     EDTPAISQPEVNEFHELLEIFYKTA"
BASE COUNT         1040 a          634 c          770 g         1035 t
ORIGIN      
        1 ttactaccct tatgcccaga attttttttc caacgctctg gccgttcatg gtaatctcct
       61 gtaaagggtg ataaagtaaa atgggaagga atagtaataa gccaacagcc tgcatattat
      121 agttgctgtc aatgtcccag ctgctgttca tcgctatgct tttgtagatc tcgttggcta
      181 tacgcaggta aaaaaactgg atgaccatgt caatcaataa agccagcagg cgccggtaaa
      241 actccggtac ttcaaactct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn ntttaggtgg tcaaattaat gttttttacg taattaaatc
      361 cggatggatt gttttttgtc tttttaaaag cagtatttta caaccgaatt tttatttcgt
      421 tgagagaagc cttattcata aagaaaaaca aagatcgctg gttgcgtaac cagcatatgc
      481 catccgataa cccggacgaa atggctgccg attttacgca attggtagat gatctggctt
      541 atgctaaaac tttttatcct tccggtaaag taaccactta catcaattcg caggcgtcaa
      601 agatttatct caatatttac aaaaacagaa aggaggagtc gaaccgtttg gtcacattct
      661 ggaagtatga ccttccgtta actatccgca agcatcaccg ggttgttttt ttctcttttg
      721 ttttcttcct ggtatttttt gccattggat attttgtatc cctgaaagat gaagggatac
      781 caagaagtat tttcggcgat gactatgtgg atcataccaa agaaaatatt gcaaatggca
      841 acccgtttgg catttacgaa catggtaacc cggtactaag ctggctgagc ctgatgatcc
      901 ataatatcag ggtttcattc cttatgttcg tctccggaat tttttgtggc atacccagtt
      961 tatatatgga agccagcaat gcggtgatgc tgggctcttt tgaccagttc tttactgaaa
     1021 ggggattagg ggtagatttc tggctggttg tttttgtaca cggaacattg gaaattaccg
     1081 ctttgattat tgcagcagca gcaggtcttg ttttgggtaa aagtttcctt ttccccggga
     1141 ccatccggcg gattgatgct ttcaagcagg gtgcaaaaga cggagtaaag atcatggtag
     1201 gcctcatacc agtatttggc ctcgccgcct tttttgaagg gtttattaca aggttataca
     1261 acgatatctc cgctttgacc acactcatat ttgcgttatc ggtggttttc gttgtctggt
     1321 attttattat ttatccgatc cgcttaagta aaaaactgtc gttgcagtta caagaggagg
     1381 aagtttaagt attgaaaccg atcagcacat atatccgcat tatattttgt tatagaatta
     1441 tcgttggcat ttttctgttt acggccagtt cgtcgatact agcacagtct gctgatgaaa
     1501 tggatacttc ggtaattgaa gaagtagtgc ctgcagatga atattcagat gacgaagaaa
     1561 aatcggccga tgacgaatat ttctgggaaa aggaatacca ggatgatagc tggaagcagc
     1621 ataagcagcg gaaagtttct gacagtgcta tgaaaaagat gcaggaggat gatgatttct
     1681 ggtatgctaa tgctgttttt aataaagaaa ataagaagga caaaaatcag ggagggtata
     1741 taccgcttgg tcagcgccaa tggttccaaa cattgatttg gataattatc gttggaggct
     1801 ttgttacgtt tctcatcatg tacctcgcag gaacagaggt aggcttgttt cgtaagaaaa
     1861 gtgttcctgt taagagccgg gaagaagagg aagaaatgcc tgaagatatc tttgctatta
     1921 actatcagaa agaaattgac aaggcagcag cacagggtaa ttaccgcctt gctgtcaggc
     1981 tgatgtacct gcgtctcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnttac ctgatgcagc tttcttcaac aaagtattac
     2101 aatgattttt tccgtatcac ccgccattat gaatacagct ggtatggaaa atttgaagtt
     2161 agcggcgaag cctaccaatt aataaacaag gaagtgctgc agtttgaaaa ccagttacga
     2221 cagaattgaa aagtaaactt ccttatataa tccttgggct tgtcgctgtt ggcatactga
     2281 tgctggtggc aacaggtaac tacaaaaaga acaggcattt tgatgggcgc attacgctgc
     2341 gtaagtcgga taaaatacca tatggctctt atgttgccta ccaaaatctt aaaagtattt
     2401 tccccggcgc aactatatat accgaccggc atgaacctgg ttactgggat tcactttcta
     2461 attatgaaaa aaagcaggct gttataatcc tagccggacg ttttgcagcg gatgaatttg
     2521 agatgaagca gctgattaag tttgcagaaa aagggaatga tgttttcgtc atagcgatgg
     2581 aaatatctgc cacggctgag cgaatgttag gttgcgatgc tagaaattat tatctcgcct
     2641 cttttttcga atcaggtgag aatcccggaa aagatacact cacattacaa ttgaaagatg
     2701 tcgacaatca aagaataaca caatacagat atccggggcg atcctttaac gggtatttca
     2761 atgaggtaga caccgttact acacatgttt taggtaccga tgcagtaggg agagcaaatt
     2821 ttatccactt gcaggcaggc gatgggaatt tttacatcaa tatggcaccg gtagctttca
     2881 gcaattattt tttgctgcat ggtcagaata ttggttatta tgaaaaggca ctgtcttata
     2941 tcaggcctga tgtacagaag atcgtttggg atgagtatta cctgaataag aaatcaggtg
     3001 gccaggacaa tgaaaaaaag aagggatggt tcactgtact gatgaacctg aaaaatgaag
     3061 aaggcaaaaa gccatttcgt gccgctttct ggctggcgat attactctta tttctctatg
     3121 tactcatgga aatgcggcgt aagcaacgat atatacccgt tgttgcaaaa ccccgcaacg
     3181 attcaatgga ttttgtcaaa accattggcc gtttatacta tgataaaggc gatcatcgaa
     3241 acctttgtct gaagatgagc agttatttcc tggagtatgt aaggaacaaa tacaagttgc
     3301 ctaccagcaa actggatgag gagtttatca tgaacctacg ttataaatca ggcgttgaag
     3361 agccagtggt cagaagtatt atttccttta ttaaatatgc tgaggataca ccggccatat
     3421 cacaacctga agtcaatgag tttcatgaat tattagaaat cttttataaa accgcttaat
     3481 aatggacgat caaatttttc aacaacggac cgatctttct gctttgaatg aggcagtatt
     3541 gtcaattcga aatgagataa agaaaatcat cgtaggacag gatgaaatgg taagacttat
     3601 tattacc
//
LOCUS       DAORLS010000304         3592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14136_length_3592_cov_1.655923, whole genome shotgun sequence.
ACCESSION   DAORLS010000304 DAORLS010000000
VERSION     DAORLS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14136_length_3592_cov_1.655923"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    309..455
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<456..>1032)
                     /locus_tag="PK977_16960"
     CDS             complement(<456..>1032)
                     /locus_tag="PK977_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19870.1"
                     /translation="TESEKRYLVNTGRSVLAFLHRETEPFTYAENNEVTYFELERGLG
                     IALYSMNAERRLSIESYIGYLAFKNGTPVAYGGGWLFGQRCQFGINILPPFRGGESAF
                     LFYQLLRVYKQYFSAERFVVKPYQFGKNNTEALQSGAFWFYYKAGFRPEDNTLQQLAI
                     TEYKKRKKDKQYRTPVHLLKKFTSSNLCLDFS"
     assembly_gap    1033..1064
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1065..1794)
                     /locus_tag="PK977_16965"
     CDS             complement(<1065..1794)
                     /locus_tag="PK977_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19871.1"
                     /translation="MLAQQKRYDQQLEELRSVALQMEPGTALKKSRLLAALTNKPPVK
                     AKDLLAYHDCLLLMLAYPANKTLLKATTTALFILKNKLTEIFSGNNSRLQRSLAGSGI
                     AGSTITGSFSFAITKWLSENFPDNVEIESSRADPESVRISFSQILPQTEYESIFCGEL
                     GLIKRIQKLKSKTKGSLINWLIHQIDSSSLSDRDKEAFFHHLQIFISWKLNNECFNRT
                     RSAGLNIKTFFHRTINKQADVKKIV"
     gene            1922..>2158
                     /locus_tag="PK977_16970"
     CDS             1922..>2158
                     /locus_tag="PK977_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19872.1"
                     /translation="MKTNNEIVSPPELIEVSGARVHNLKNIDISIPKNKLVVITGISG
                     SGKSSLAFDTIYAEGQRRYMETFGAYARQFIGDME"
     assembly_gap    2159..2401
                     /estimated_length=243
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2415..>2756
                     /locus_tag="PK977_16975"
     CDS             2415..>2756
                     /locus_tag="PK977_16975"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF19873.1"
                     /translation="MAPLVRGRKGHYRELFEEMRKKGFLKVRIDGEIKDLVPKMQVDR
                     YVIHDIELVVDRLQVTEDLKARLSQSVQQTLKLGKDLLFLLVDDSKFVQYSKLLMCED
                     TGLSYEEPSPNA"
     assembly_gap    2757..2807
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2808..3217
                     /locus_tag="PK977_16980"
     CDS             <2808..3217
                     /locus_tag="PK977_16980"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF19874.1"
                     /translation="KGLGNVYTINMDLVLPNKDLSVREGGIAPLGEERDASVFQQVIA
                     FAKKNKISLDKPVKELPKNHLDLLLFGDKGISPSLTVDDDESIPLEYTGSYEGIIPML
                     KRWFISPAVLIFCGSGWRNIWNWPFATVVTGPG"
     gene            3175..>3592
                     /locus_tag="PK977_16985"
     CDS             3175..>3592
                     /locus_tag="PK977_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF19875.1"
                     /translation="MELAVCHSCNGARLKKESLWFRVNGRNISELSNMNLDNLAAWFD
                     GIELMLDNKQKAIAKDVLKEIRERLQFLLDVGLTYLTLNRPSKTLSGGESQRIRLATQ
                     IGSQLQGITYILDEPSIGLHQRDNHRLIEALKNLRDI"
BASE COUNT          903 a          578 c          695 g          943 t
ORIGIN      
        1 cttaaaataa attccaaaca cttgtttgta tatttcctga aaccctttac tgtaaagggt
       61 ttcattgggg aaaagcggac gaatattttt gcaaaatgga aagagtcaat gctgaaagaa
      121 atgacttatc cagttcggcc caaaaaagct tatgtttttg tattagcctg atgaagttta
      181 tttccgggaa atttcctttc gccctgatta ttcggacaag taattttttt tctttatccg
      241 tccatttttc tatgttaagt agtgcctgaa caaataagca ccattcgcgg aggacttttt
      301 tttcatgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaaa atcaagacat aaattcgatg
      481 aggtaaactt tttaagcaag tgcactggcg tccggtattg cttatctttt ttcctttttt
      541 tatattctgt tattgccagt tgttgtaatg tgttgtcctc cggcctgaac cctgctttat
      601 aataaaacca aaaggcacca gattgtaatg cttcagtgtt atttttccca aattgatagg
      661 gtttgactac aaaccgctct gcactaaagt attgtttata tacccgtaat aactgataaa
      721 ataaaaaggc tgactcccct ccccgaaacg ggggtagaat attaataccg aactggcagc
      781 gttgtccaaa aagccagcct ccgccataag caacaggggt tccgttttta aaggcaaggt
      841 atccaatata ggactcaata gatagtctcc gttctgcatt catactgtac aacgcaatgc
      901 caaggcctct ttccagttcg aaataagtta cttcattgtt ttcagcatag gtaaagggtt
      961 ctgtctcccg gtgcaggaaa gccaatacgg atcggccagt attgaccagg tatctttttt
     1021 cgctttcggt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaacaa ttttttttac
     1081 atcagcctgc ttgttaatag tgcgatgaaa aaaagtttta atatttagtc ctgcacttcg
     1141 ggttctgtta aaacactcat tgtttaactt ccagctgata aatatttgaa gatggtgaaa
     1201 aaaagcttct ttgtctctgt cagaaaggct gctggaatca atttggtgta tcagccagtt
     1261 tattagagaa ccttttgttt tgcttttaag tttttggatg cgtttaatga ggccaagttc
     1321 accgcaaaaa atactttcat actccgtttg cggcaggatc tggctgaagg atattcggac
     1381 tgattccggg tctgcccggc tgctttcaat ttcaacatta tcggggaaat tttctgataa
     1441 ccatttggta atagcaaagc tgaatgaacc agtaattgtt gaccctgcta taccactgcc
     1501 agccagtgat cgctgcagac gggaattatt acccgagaat atttcagtta atttattttt
     1561 taatatgaat agggctgttg ttgttgcttt aagcaaagtt ttattggcag ggtatgccag
     1621 catcagcaaa aggcaatcat gataagccaa caggtctttt gccttgaccg ggggtttatt
     1681 ggttaacgct gccagcagcc tgcttttttt cagggcagtt cctggctcca tttgcagggc
     1741 aactgatctt agttcttcca gctgttgatc gtatcttttt tgttgggcaa gcattagtta
     1801 ccctaaaaaa atcatttaag atgcaagaaa gcctgatttt ttgatgacaa taacctgatt
     1861 tctgtcaatc agaacagcaa ctttccggga ggttctttgt tcttaccttc gttatccagc
     1921 aatgaaaacg aataatgaaa tagttagccc cccggagctg atagaagtgt ccggtgcccg
     1981 ggtacacaac ctcaagaata ttgatatttc cattccaaag aataagttgg tggtaattac
     2041 cggtattagc gggagcggaa aatcttcttt agcatttgat accatttatg cggaaggtca
     2101 gcgacgctac atggaaacat tcggtgctta tgcccgccag tttatcggtg atatggaann
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 naaaaattgc cttattggcg cctctggtca ggggaagaaa agggcattac cgggagttgt
     2461 ttgaagaaat gcggaagaaa ggttttttga aagtgagaat cgatggagag ataaaagacc
     2521 ttgttccaaa aatgcaggta gacaggtatg ttatccacga catagaactg gtggtagaca
     2581 ggctgcaggt tactgaggac cttaaagcga ggctaagcca aagtgtacaa caaacactga
     2641 agttgggcaa agacctgcta tttttactgg tggatgattc aaagtttgtt cagtattcta
     2701 aattgttgat gtgtgaggat acgggtctca gttatgagga gccatcaccc aatgctnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta agggtttggg
     2821 taatgtgtat acgatcaata tggaccttgt attgccaaat aaggatttat cggtgagaga
     2881 aggtggtatt gcacctttgg gagaggaaag agatgccagt gtttttcaac aggtaattgc
     2941 tttcgccaaa aagaataaaa taagccttga taagccggta aaagagttac ctaaaaatca
     3001 tcttgatctt ttactcttcg gcgataaggg aatcagtcct tctttaactg tcgatgatga
     3061 tgaatcaatt cctttggaat acaccggcag ttacgagggc attattccaa tgttgaaaag
     3121 atggtttatt tccccggcag tactgatttt ttgcgggagt gggtggagaa atatatggaa
     3181 ctggccgttt gccacagttg taacggggcc cggttgaaaa aagaaagtct ttggttcagg
     3241 gtaaacggaa gaaatatttc cgaattaagc aacatgaacc tggataacct ggccgcctgg
     3301 tttgatggca ttgaactcat gctggataac aaacaaaaag caattgccaa ggatgtactg
     3361 aaagaaatca gggagcggtt acaattttta ctggatgtag gattaacata cctcactctt
     3421 aaccggcctt ctaaaacact cagcggaggc gagtcgcagc gaatacgatt agctacacaa
     3481 atcggttcac aattgcaggg tatcacttat atactggatg aaccttctat tgggttgcac
     3541 cagcgggata atcaccggct gatagaagca ttgaaaaacc tgagagatat tg
//
LOCUS       DAORLS010000305         3586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14165_length_3586_cov_2.271878, whole genome shotgun sequence.
ACCESSION   DAORLS010000305 DAORLS010000000
VERSION     DAORLS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14165_length_3586_cov_2.271878"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="dprA"
                     /locus_tag="PK977_16990"
     CDS             <1..839
                     /gene="dprA"
                     /locus_tag="PK977_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRF19876.1"
                     /translation="QRLLNCYDSPTLLYYKGEADLNASRIIAIIGTRSHTEYARQVTE
                     KIVKELSEENVTIISGLAYGVDAIAHKAALKNNLPTIGVLAHGLDQVYPPDHTGLAKD
                     MVKNNGGLLTEFRSKTKPDKHNFPTRNRIVAGMSDATIVIETGIKGGSMITAEMANSY
                     NKDVFAIPGKITDSKSAGCNYLIKNNKAILLTDTAELLQLMGWQQARKKERKVQREIF
                     IELSKDEKTIIDILKQKDNVHIDEINILSGISSSAIAAAILSLELQNVILSLPGKLYK
                     LA"
     gene            883..>1520
                     /locus_tag="PK977_16995"
     CDS             883..>1520
                     /locus_tag="PK977_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF19877.1"
                     /translation="MRKKLTTLLIVLTFWVTAAHAQPCTTLGQTPSTAFPVCGTTTFT
                     QATVPLCSTTSLFVPGCTGDGANYENKNPFFYKFTCYVGGTLGFVITPLAANEDYDWQ
                     LYDITGKNPDAIFTDRTIIVTGNWSGSYGPTGASASGVNYIQCASVPSDNLPTFAQMP
                     VLIQGHEYILMISHYTDTQSGYTLAFGGGTAVITDPKVPDMLSAKPDCDGRS"
     assembly_gap    1521..1650
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1651..2912
                     /locus_tag="PK977_17000"
     CDS             <1651..2912
                     /locus_tag="PK977_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF19878.1"
                     /translation="DFEEITLTLAAALPNGTYDLVINNGTDANTLLDLCGTGIAAGNR
                     VSFTYAAPQPIFADSIGKVPCTSDSVKVYFPKKISCASIAPNGSDFSVTGPAPVTVVS
                     AAGNCVNGKTDYVVVKFASPIYTRGIYTLNLKAGNDGTVLIDECGQETPVQSLPFNTA
                     DTVNAEFNFTSRFGCQRDTLTFSHNGAHDVNFWSWNFNSDPPVLTQTHTIIFPAFSTN
                     NVQLIVSNGTCTDTASATIVLNNEVKADFTMDSFICPEDKLTVINNSRGNVDAWKWNF
                     DVIATSTLKDPLPVQFPTLNREAYYTVKLVAYNNSLRCADSMKKTLTVLDHCRIAVPT
                     AFTPNNDGRNDFFWPHNALKADNLEFRVYNRWGQLVFQSQGWRDKWDGTLKGIQQPAG
                     VYVWMLRYVHRETKKEVFEKGTVMLIR"
     gene            2967..>3586
                     /locus_tag="PK977_17005"
     CDS             2967..>3586
                     /locus_tag="PK977_17005"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRF19879.1"
                     /translation="MATRNSSPAASKSQRFFGEGLTFDDVLLMPGYSQVLPRDVNIKT
                     KLTKNITLNVPLLSAAMDTVTEASLATALAREGGLGILHKNMSIEKQAEQVRKVKRSE
                     SGLIIDPVTLQADATIGDALRLMRENKIGGIPIIDKTGKLVGILTNRDLRFEDDPKRK
                     VSEVMTKENLFTAPEGTDLKKAEQLFKKTKVEKLPIINKQGKLTGL"
BASE COUNT         1085 a          767 c          749 g          855 t
ORIGIN      
        1 cgcagcgttt actaaactgt tacgactctc ccaccctatt atattacaag ggagaagctg
       61 atctgaatgc ttcccggatt atcgctatta ttggtacccg aagccatacg gagtatgcga
      121 gacaagtaac agaaaaaata gtaaaagaac tgtcagaaga aaatgtcacg ataatcagcg
      181 gcttagctta cggtgttgat gccattgcac ataaagctgc cttaaaaaat aatcttccca
      241 cgattggtgt actggcgcat ggacttgacc aggtttaccc tccggaccat acgggactag
      301 ccaaagacat ggtgaaaaat aacggaggat tgctgactga gttcaggagt aaaacaaaac
      361 ctgacaaaca taattttccg acaagaaacc gcatcgtagc cggcatgagt gatgctacta
      421 ttgtaatcga aacaggtatt aaaggtggca gcatgattac agcagaaatg gcgaacagtt
      481 acaacaaaga cgtttttgcc atacccggga aaattactga tagtaaaagt gccggttgca
      541 attacctgat caaaaacaat aaagccattt tattaactga cacagcagag ttattgcaat
      601 tgatgggctg gcagcaggcc aggaaaaaag aaaggaaagt tcagcgggaa atatttattg
      661 aactcagcaa agatgaaaaa acaatcattg atattctaaa acagaaagac aatgtacata
      721 ttgatgagat caatatcctg agcggcatca gcagcagtgc gattgccgct gccattctta
      781 gcttagaatt acaaaatgtg atactatctt taccgggcaa attatacaag cttgcataac
      841 ccgcagcaag acaaaacagc tgacctggcc atgaaaatat ttgtgagaaa gaaattaaca
      901 acactgctga ttgtactgac cttttgggtt actgctgctc atgcccagcc ttgcactact
      961 ttaggacaaa ctccttctac agcttttccg gtttgcggca ctaccacatt tacacaggcc
     1021 acagtacctc tttgcagtac aaccagcctg tttgttcccg gttgtacagg cgatggtgcc
     1081 aattatgaaa acaagaatcc atttttttac aaattcacct gctatgttgg aggcacattg
     1141 ggttttgtca tcacgccact ggctgccaat gaagattacg actggcagtt atacgatatc
     1201 accggcaaga accctgatgc catatttacc gacagaacga tcatcgtaac aggtaattgg
     1261 tcaggctctt atggcccaac cggcgcttcg gcttctggtg tcaattatat tcaatgtgcc
     1321 tcagtaccca gtgataacct tcccacattt gcacaaatgc cggtactgat tcaagggcat
     1381 gaatacattt taatgatcag ccattatact gatacacaaa gtggatatac actggcattt
     1441 ggcggcggca ctgctgttat taccgacccg aaagtaccgg atatgctaag cgccaaacct
     1501 gattgtgacg gaagatccat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgattttga agaaataacg cttacactgg
     1681 ccgctgcatt acccaatggc acatacgacc tggtgatcaa caacggtacc gacgcaaaca
     1741 ctttgttgga tttgtgcgga acaggaattg cagcaggcaa cagggtaagc tttacgtatg
     1801 cagcaccaca acccattttt gcagacagca taggaaaagt gccctgtaca tcggattcag
     1861 taaaagttta tttcccaaaa aagatcagct gtgccagcat tgcacctaat ggcagcgatt
     1921 ttagtgtaac cggccctgca cctgtcactg ttgtcagtgc cgcaggtaat tgtgtaaatg
     1981 gaaaaacaga ttatgtggta gtgaaatttg cttcgcctat ttatacaaga ggaatatata
     2041 cactaaacct gaaagcaggt aatgacggaa ccgttttgat tgatgaatgc gggcaggaaa
     2101 ccccggttca atcacttcct ttcaatacag ctgacacagt gaatgctgaa tttaatttca
     2161 cttccaggtt cggctgccag cgggacacat tgactttttc tcacaatggg gcccatgatg
     2221 taaacttctg gagctggaat ttcaacagtg atcccccggt attaacgcaa acccatacta
     2281 ttattttccc tgcattcagc accaacaatg tgcaactgat tgtaagcaac ggaacctgta
     2341 cagatacagc gtctgcaaca atcgttttga ataatgaggt aaaggcagac tttacgatgg
     2401 actcttttat ttgccccgag gataaactca ctgtcattaa taacagccgt ggcaatgtag
     2461 atgcctggaa atggaatttt gatgtcatag ccaccagcac actgaaagac ccgttgcctg
     2521 ttcaatttcc aacattgaat agagaagcct actataccgt aaaactggtt gcttacaaca
     2581 acagcctgcg ttgtgcagac agtatgaaaa aaacactaac tgtactggac cattgccgaa
     2641 ttgccgtacc tactgctttt actcccaata atgatggccg taatgatttc ttctggccgc
     2701 acaatgcatt aaaagccgac aacctagagt tcagggtgta caacaggtgg ggccaattag
     2761 ttttccagag ccagggctgg cgggataaat gggatggcac cctaaaagga atacaacaac
     2821 ccgcaggagt gtatgtgtgg atgctgcgct atgtgcaccg ggaaacgaaa aaggaagtat
     2881 ttgaaaaagg aactgttatg ctaatccggt aagacctctt tcgaaaaatc atttttatca
     2941 tttgactcag cggtctatct ttgcacatgg caacaagaaa ttcttccccc gctgcaagta
     3001 aatcccaacg tttttttggt gaaggcctga ccttcgatga tgtattgctg atgcccggct
     3061 acagccaggt attgccccgt gatgtaaaca tcaaaaccaa actgacgaaa aacattacgt
     3121 tgaatgttcc gttgctttct gcggcaatgg ataccgtaac agaagcttct ctggctactg
     3181 cactggcaag agaaggcggc ctcggcatcc tgcacaaaaa catgagcata gaaaagcagg
     3241 ccgagcaggt tcgtaaagta aaaagaagtg aaagcggcct gatcatcgac ccggtgacat
     3301 tacaggcaga tgccacaatt ggtgatgcat tacggctaat gcgtgaaaac aagatcggcg
     3361 gtattcctat tatagataaa acaggaaaac tggtgggtat tcttaccaac cgtgaccttc
     3421 gttttgaaga cgacccaaaa cgaaaagtga gtgaagtgat gaccaaagaa aatcttttca
     3481 ctgcacctga aggaacagac ctgaaaaaag cagaacagct tttcaaaaaa accaaagtag
     3541 aaaagcttcc gatcattaat aagcagggta aactcaccgg cctctt
//
LOCUS       DAORLS010000306         3581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14195_length_3581_cov_1.510777, whole genome shotgun sequence.
ACCESSION   DAORLS010000306 DAORLS010000000
VERSION     DAORLS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14195_length_3581_cov_1.510777"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK977_17010"
     CDS             complement(<1..369)
                     /locus_tag="PK977_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF19880.1"
                     /translation="MKPAIEIRNLGKQYKLTSAEPYIALRDVITHGVKNIFSSRKKTS
                     EKFWALQDINLDIMPGERVGIIGRNGAGKSTLLKIISRITPPTTGEAIIRGRVGSLLE
                     VGTGFHPELTGRENIYLNGSI"
     gene            complement(548..>927)
                     /locus_tag="PK977_17015"
     CDS             complement(548..>927)
                     /locus_tag="PK977_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF19881.1"
                     /translation="NLFSSSVSHAGESMIQNANIINKIYFPRLIIPGSSILVALFDFL
                     MGFIIFIIFCAVYQQSVDWSAVVYFPAGIFLLLTAAFGLGTFLAALNVKYRDFRYTIP
                     FLLQVLFFASQVIYPFIRYNKPG"
     assembly_gap    928..1080
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1246..2223)
                     /locus_tag="PK977_17020"
     CDS             complement(1246..2223)
                     /locus_tag="PK977_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19882.1"
                     /translation="MKRLTIIITILLLAFSSSAQKISSADLKKLRAKEDTLKEYAEYI
                     VTDSLTEDRMIADSAFTKVLVRALQVKNSFYYPFDSVLGVAKIYAPDTSFRIITWNLV
                     FDDYYSRQRGAIQMRTADGSLKLFPLRDVSEFTSKPEDSVRNRNNWIGAMYYNMVKTQ
                     HKGKNYYTLFGFDYNSAQSSMKWIEVLTFNERNEPVFGGPFFSYEQDSIPKPPKYRFS
                     TEFKKGARVLVNYIEELDMILVDHLISETDQPELAWTFVPDGDQEGYKWENGKWLHID
                     KVFTFKLQDGQAPIGDPLLDPKGNRNEQKLQEKSDKNKTKEKSQINYDN"
     gene            complement(2310..3575)
                     /locus_tag="PK977_17025"
     CDS             complement(2310..3575)
                     /locus_tag="PK977_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRF19883.1"
                     /translation="MIKEAIILAGGLGTRLRDTVPDLPKCMAPVAGRPFLFYVINYLR
                     SQGLEKFIFSLGHMHEIIEQYLENNFPTLNYQCSIEKEPLGTGGAILLACTKATEKQV
                     AVTNGDTLYKANLHNAAIFHMHHMAECTLMLKPMTDFDRYGVVETDKEKLVTSFKEKQ
                     YYKEGNINGGLYLLDTGRFMDEEFPQKFSFEKDYLEKLYNKRRIYGMTDKSYFIDIGI
                     PEDFNRAQNELAKAPLDLEKTDKTWTLFLDRDGVINHEKKEDYILNWEEFRFYEGVKE
                     TFHLLAKKFGKIIIVSNQRGVGKGLMTEADLRDIHFNMQQEIESAGGRIDHIYYCTST
                     DNKHPDRKPNPGMAFHAQSFFSEIDLDKAIVVGNKPSDMQFGRNASIYTVFAATTNPE
                     VPYPHPDIDLRFGSLADFARACLPVDKTL"
BASE COUNT          957 a          721 c          727 g         1023 t
ORIGIN      
        1 aatagagcca ttcaggtaaa tattctcccg gccagtcagt tccgggtgaa agccggtccc
       61 cacttccagt aaactgccca cccggccgcg gataattgct tctccggtag taggaggggt
      121 aatacggctg atgattttta ataaagtgct tttgcctgca ccgttccggc cgattatgcc
      181 aaccctttcg ccgggcataa tatcgagatt aatgtcctgt aaagcccaga atttttcgga
      241 agtctttttt ctgctagaaa aaatattttt cacaccatgg gtgataacat cccgcagtgc
      301 aatatatggt tctgcacttg tcagcttata ttgcttaccc aggtttcgta tttcaatggc
      361 aggtttcatc aggcgaggtc agcaaaatag ctttctgttt tacggaaata aaatagcccg
      421 gttataaata ataaaaggct gctcgttaat cctattatca aggttgcggc attggcctcc
      481 ccaccggtca acggtaagcg gaacagttca atagccgcat tcattggatt aacagccagc
      541 aggtatttta accaggtttg ttgtatcgaa tgaaagggta gataacctgt gatgcaaaaa
      601 ataaaacctg taaaagaaac gggatagtat agcggaagtc ccggtacttt acatttaatg
      661 cggccaaaaa tgttccaagg ccaaatgcgg ctgttaaaag caggaatatt ccagccggaa
      721 agtatacaac tgcagaccag tctaccgatt gctgatatac tgcacaaaaa ataataaaaa
      781 tgataaagcc cataaggaaa tcgaatagtg ctacgagtat tgatgaaccc ggaatgataa
      841 gccttggaaa atagatctta ttaatgatat tggcgttttg tatcatgctc tccccggcat
      901 gtgatacgga actgctgaac aggttccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 gtttgtattt tacttttata tcccgccagg taaagaaata aaagagttca cggtattgcc
     1141 ataactcttt caaacccaac gaaaatcccg aagctggctt tattctatga acgaaatcgg
     1201 acattggcat aaagatagcc aatgaaggcg taaagtgaaa aggagttaat tgtcgtaatt
     1261 gatctggctt ttttcttttg tcttgttttt atccgatttt tcctggagtt tctgttcgtt
     1321 acggtttccc ttcgggtcca gcaaaggatc accaataggg gcttgtccat cctgcagttt
     1381 aaaagtaaag accttatcta tatggagcca tttaccattt tcccatttat aaccttcctg
     1441 gtcgccatcg ggtacaaatg tccaggccag ttcaggctga tctgtttctg aaataaggtg
     1501 gtcaacgagt atcatgtcca gttcctcaat atagttaacc aaaacccggg cgcctttttt
     1561 aaactcggta ctgaaacggt actttggcgg cttgggaatg ctgtcctgtt cgtagctgaa
     1621 gaatgggccg ccaaaaaccg gctcattcct ttcattgaaa gtgagtactt ctatccattt
     1681 catactgctc tgggcactgt tgtaatcaaa gccaaacaag gtgtaataat tttttccctt
     1741 gtgctgtgtc ttgaccatat tataatacat ggctccgatc cagttgttac ggttgcggac
     1801 agaatcttcc ggcttactgg tgaattccga tacatctctc aacgggaata attttagtga
     1861 tccgtccgct gtacgcattt gtatggcacc ccgttgccgg gagtagtaat catcaaaaac
     1921 aagattccag gtaatgatcc ggaaggaagt gtcgggggca tatattttgg caacgcccaa
     1981 tacagaatca aaaggatagt agaaggaatt ttttacctgc agggccctta ccagcacttt
     2041 ggtaaaggca ctgtcggcga tcatacggtc ttcggtcagg ctatcggtaa cgatatattc
     2101 ggcatactcc tttaatgtat cctctttggc ccttaatttt tttaaatctg cagaactgat
     2161 cttttgagca gaagaagaaa aagccagaag taaaatcgta attatgatag taagtcgctt
     2221 cacccgggtt tattttaatc caatttgcta agatacagtc agaaagagat gaaactaacg
     2281 ccaaaagggt tgtaaaattt tgtgaaggat cataaagtct tgtcaacagg cagacaggcc
     2341 ctggcaaaat cagccaatga accgaaacga aggtcaatat cagggtgggg gtaaggtact
     2401 tcgggattag tggtggctgc aaatactgtg taaatgctcg cattacggcc aaactgcata
     2461 tcacttggtt tattcccaac cacgatcgct ttatccaggt caatttcgga gaagaatgat
     2521 tgggcatgaa aggccatacc cgggttgggt tttctgtcag gatgcttgtt atcggtgctg
     2581 gtacaataat aaatatggtc aattctgcca ccggcggatt caatttcttg ctgcatgttg
     2641 aaatgaatat cccgcagatc tgcttccgtc atcaggcctt ttccgactcc ccgctgatta
     2701 cttacgatga tgatcttgcc aaattttttt gccagcaggt gaaaggtttc tttcacacct
     2761 tcataaaacc tgaattcttc ccagttaagg atataatctt cttttttttc atgattgatc
     2821 accccatccc ggtcaaggaa aagggtccag gttttatcag ttttttccag atcaaggggt
     2881 gcttttgcca gttcattttg tgccctgtta aaatcttccg gtatgccaat gtctatgaaa
     2941 taagacttat ccgtcatgcc atatatgcgc cgcttgttat atagtttttc gagataatct
     3001 ttttcaaaag agaatttctg cggaaactct tcatccatga atcttccggt atccagcagg
     3061 taaaggccac cgttgatatt tccttctttg tagtattgct tttccttaaa acttgtcact
     3121 aatttttcct tatctgtttc tacgacacca taacggtcaa aatcagtcat gggcttaagc
     3181 atcaaagtac attctgccat gtgatgcata tgaaaaatgg ctgcgttatg caggttggcc
     3241 ttgtataatg tatcgccatt ggttactgcc acctgttttt ccgtggcttt ggtgcaggcc
     3301 aataaaatgg caccgccggt tccgaggggc tctttttcaa ttgaacactg ataattgagt
     3361 gtggggaaat tattttccag gtattgctca atgatctcat gcatatggcc aagagaaaaa
     3421 atgaattttt caagcccctg gcttcttaaa tagttaataa catagaaaag aaagggtctt
     3481 cccgcaacag gagccatgca tttgggcaga tcaggaacag tatcacggag ccttgttcct
     3541 aaaccacctg ctaaaataat tgcttcttta atcataaaga c
//
LOCUS       DAORLS010000307         3572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14244_length_3572_cov_1.817174, whole genome shotgun sequence.
ACCESSION   DAORLS010000307 DAORLS010000000
VERSION     DAORLS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14244_length_3572_cov_1.817174"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PK977_17030"
     CDS             complement(<1..579)
                     /locus_tag="PK977_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19884.1"
                     /translation="MNNIHIPKEVLEAFAIDNAGNVIIESIGNGLINDSWKIVTVNNG
                     NIFLQRINKNVFHEPQLVQANYIQLWKYAKTANKGLLMPDPVFCNNQTSLHTDPLGNY
                     WRAFYFIPESKTCPVAETATQAWATAKTFGQFTAAFSEMDTSLLHEVISNFHNLSLRY
                     RQFETSLQSRSVERLARASSLIADLKKREHYKT"
     gene            complement(696..1304)
                     /locus_tag="PK977_17035"
     CDS             complement(696..1304)
                     /locus_tag="PK977_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HRF19885.1"
                     /translation="MELSQDAKNILGLQLPTDPRWVNLAEKSLEEVLTDHAYCEQKAA
                     TSCISLIQRYSRCEKLVTELSPIVTEEWGHFRLVLAELQKRNLKLGKQRKDEYVNALI
                     DFQQKGGDADGRMLDQLLTMALIEARSCERFKRLSEGLNDEYMQKFYRRFMESEAGHY
                     TLFIELAETYIDKEKVRRRWKEWLAYEAEVIKNLEVRGDRIH"
     gene            1463..1732
                     /gene="rpsO"
                     /locus_tag="PK977_17040"
     CDS             1463..1732
                     /gene="rpsO"
                     /locus_tag="PK977_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRF19886.1"
                     /translation="MPLAKEKKAAVLKTYAGSEKNTGSIEGQIALLTERISQISGHLQ
                     QNKKDFSTHRGLMQLVGKRKRLLNYMQKHNLMGYRQLIEKLGIRK"
     gene            2042..2887
                     /locus_tag="PK977_17045"
     CDS             2042..2887
                     /locus_tag="PK977_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19887.1"
                     /translation="MLQQPIRKTFDIGDGRMVTIETGRLARQADGAVTVSQGNCMILA
                     TVVANKEPKEGQSFFPLSVDYQEKFAAAGRIPGSFFKREAKLNDYEVLTSRLIDRALR
                     PLFPEDYFCDVQVLVTLVSSDPEVMPDSLACLAASAALAVSDIPIKEIISEVRVSRID
                     GQFVVNPTRTQMEKSDMDFIIAATEKNLMMVEGEAQECSEADLVKALGIAHDAIRIQV
                     KAQEELRDLAGVKGKRDYTKPATDEALREKIYAFAKQKVYEVAKAGLSKHERSDRFDA
                     IKWNI"
     gene            2875..>3189
                     /locus_tag="PK977_17050"
     CDS             2875..>3189
                     /locus_tag="PK977_17050"
                     /inference="COORDINATES: protein motif:HMM:NF015671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRF19888.1"
                     /translation="MEYLKKEYNVAEGESLPDLTKKLASTYYGDLQYDVVRDMILDEK
                     IRLDGRDLETVRPLDMEVDVLPSPHGASLFTRGETQSLTTVTLGTPLDELLVESAAKS
                     IDS"
     assembly_gap    3190..3265
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3300..>3572
                     /locus_tag="PK977_17055"
     CDS             3300..>3572
                     /locus_tag="PK977_17055"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRF19889.1"
                     /translation="MKKVAPSETDFPYVIRIVSDVLESNGSSSMATVCAGSLALMDAG
                     VPVPKHVSGVAMGLITKGDKYAVLTDILGDEDHLGDMDFKVIGTRDG"
BASE COUNT          943 a          735 c          840 g          978 t
ORIGIN      
        1 tgttttatag tgttcccttt tctttagatc ggcaatcaac gaggaggccc ttgctaaccg
       61 ttcaactgac ctgctttgaa gagaagtttc aaactgccgg taccgtaagc taaggttatg
      121 aaaattgctg attacctcgt ggagcaggct tgtatccatt tctgaaaatg cagccgtgaa
      181 ttgtccgaat gttttagccg ttgcccatgc ctgcgttgct gtttctgcca cagggcatgt
      241 tttactttct ggaatgaaat aaaaagcccg ccagtaatta cctaaaggat ctgtatgcaa
      301 gctggtttgg ttattgcaaa atactggatc aggcatcagc agccctttat ttgctgtttt
      361 tgcatatttc caaagctgta tatagttggc ctgcaccagt tgcggctcat gaaaaacatt
      421 cttattgatg cgctgtaaaa aaatgtttcc attatttaca gtaacaattt tccaggagtc
      481 attgataagg ccgttaccaa tggattcaat gataacgttg ccagcattgt caatagcaaa
      541 tgcttccaat acttctttcg ggatgtggat attattcatt ggcaatttca actggcaatg
      601 aaattaagct gaataccaaa gtaaagttgt ttcaatcgtt tgcgggaaaa ggggagggtg
      661 ctagttgctg gttacaggtt gcttgtatgc ggacgttaat gtatcctgtc acctctcact
      721 tcgaggtttt tgatcacttc tgcttcatat gccagccatt ctttccagcg acggcggacc
      781 ttttctttgt cgatataggt ttctgccagt tcaataaaca gggtatagtg gccggcttca
      841 ctttccatga agcgacggta gaatttttgc atgtattcgt cattcagccc ttcacttaat
      901 cgtttgaatc gttcacaact tcttgcttca atcagtgcca tggtaagtaa ttggtccagc
      961 atacggccat ctgcatctcc gcctttttgt tgaaaatcaa tgagggcatt tacatattca
     1021 tctttccgtt gttttcccaa tttcaggttt cttttctgca gttcggccag taccagccgg
     1081 aaatgccccc attcttcggt aacgatggga gatagctctg ttacgagttt ttcacacctg
     1141 ctatagcgtt ggatcagcga tatgcaggaa gtagctgctt tttgctcgca gtaagcatgg
     1201 tctgtcagca cttcttccag cgatttttcg gccaggttta cccaacgggg gtcagtgggc
     1261 agttgcaggc caaggatatt tttggcatcc tgagatagtt ccatgccgca aaagtaagtt
     1321 atggcctgaa aatcagcgat aatagctgat ttggatagga aaaaaagagc aggcttccct
     1381 ttgctgttag cctgattcgc cttacctttg ccgccttaat aaagagatga aaaaaatcgg
     1441 atgaaattta aaaattaaaa agatgccatt agcaaaagaa aaaaaagctg ctgttttaaa
     1501 aacttacgcc ggttctgaaa aaaataccgg ttccattgaa ggtcaaattg ccctgctgac
     1561 tgagcggatc agccagatca gtggtcacct gcaacaaaac aaaaaagact tctccactca
     1621 ccgcggactg atgcagttgg taggtaagcg taagcgttta ctgaactata tgcagaagca
     1681 taacctgatg ggttatcgcc agttgatcga gaaattggga atcaggaagt aaaaaccgtc
     1741 cccagcccct gcaggagagt ttgggaagag tctttaaatc gccagtttct tattaaaagt
     1801 gaagctcaag atataagcta ttcccaatgt cttccggatg ttgggaataa ctgttttagg
     1861 actatcagga tttttagttg ctattgcaga cttcgcaaat gctcattagt gttaactgca
     1921 gatgaatgga gcgtcgcaac aaagctgata gtagtaatgc agttggcaaa caaaaagtat
     1981 ccttcaacaa gtttgtgagt ttataatcct aaacttcaaa ccctaaactt caaacctttt
     2041 tatgttacaa caacctattc gtaaaacatt tgacataggc gatggccgca tggtgaccat
     2101 tgaaacaggc cgtttggccc gccaggctga tggtgccgtt acggtgagcc agggcaattg
     2161 tatgattctg gcaaccgttg ttgccaataa agaacccaag gagggacaga gctttttccc
     2221 attgtcagta gactaccagg aaaaatttgc ggctgcgggc cgtatccccg gttctttctt
     2281 taagagagaa gcgaaactta atgactacga agttttgacc agccgtctta ttgacagggc
     2341 cctgcgtccg ctgttccccg aagattattt ctgtgatgtg caggtgctgg ttacccttgt
     2401 ctccagcgac ccggaagtga tgccggactc actggcttgt cttgctgcat ctgcggcgct
     2461 ggctgtatct gatattccta tcaaagaaat tatttctgaa gtaagagtat cccggattga
     2521 cggccagttt gtggtgaacc ctacccgtac gcagatggaa aaatctgata tggattttat
     2581 catcgcagcc acggaaaaga acctgatgat ggtggaaggt gaggcacagg agtgcagcga
     2641 agccgacctg gtgaaagctc ttggaattgc ccatgatgcc atccgcatac aggtaaaggc
     2701 gcaggaggaa ctgagagacc tggccggtgt aaagggcaag agagactata ccaagccagc
     2761 tactgatgaa gcgctgagag aaaagatcta tgcttttgcc aagcaaaaag tatacgaggt
     2821 ggccaaggct ggcctgagca agcatgaaag aagcgaccgc tttgatgcca tcaaatggaa
     2881 tatctgaaga aggaatacaa tgtggccgaa ggagaaagcc tgccggacct gacgaaaaaa
     2941 ttagcttcca cttactacgg cgatctgcag tatgatgtgg taagggatat gatactggac
     3001 gagaagatcc gcctggatgg ccgtgacctg gaaacagttc gaccgctgga tatggaggtg
     3061 gatgtattgc cttctccgca tggcgcttct ttgttcacaa gaggtgaaac acagtcgctt
     3121 acaaccgtta ccttaggaac tcccttagat gagttgttgg tagaaagcgc cgctaaatcc
     3181 attgattccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnagaaa tcgggcacgg aaatcttgca gaacgggctt
     3301 tgaaaaaagt tgctccttct gaaacagatt ttccttatgt tatccgtatt gtatctgatg
     3361 ttcttgaatc gaacggttca tcttctatgg ctacggtttg tgccgggtcg cttgcactga
     3421 tggatgccgg tgtaccagtt cctaagcatg taagcggtgt ggccatgggg ctgatcacca
     3481 aaggagataa atatgctgtt ctgacagata tcttaggtga tgaagaccac ctgggtgata
     3541 tggatttcaa agtaatcggt acccgggatg gt
//
LOCUS       DAORLS010000308         3540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14405_length_3540_cov_2.449067, whole genome shotgun sequence.
ACCESSION   DAORLS010000308 DAORLS010000000
VERSION     DAORLS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14405_length_3540_cov_2.449067"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>296
                     /locus_tag="PK977_17060"
     CDS             <1..>296
                     /locus_tag="PK977_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19890.1"
                     /translation="ATAFNFFLGLHIITIWLGIGGMAASLVCFYISRVKKQFSPVLIF
                     TYAIIAILLLSPLYFYNGGSNGPVFFLIIMLLFIYIFIAPSFMQGWIYIIYSIA"
     assembly_gap    297..336
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <337..1134
                     /locus_tag="PK977_17065"
     CDS             <337..1134
                     /locus_tag="PK977_17065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF19891.1"
                     /translation="FYTDWVVPYDSKEQRVIDFSIVLVYITFFTSFTIWIFRKSYDKE
                     QRTIRLQKGELEKLYQQANEKNQYIESLIKELHHRVKNNLQVVSSLMSLQSNRMKADE
                     ARNALEEGRNRVNAMALIHQKLYMDDELSSVNMNEYLENLSASLAESFGYPPAVVSPQ
                     VQLTGDKLDIDRAVSIGLIVNELVTNSFKHAFHGHTNPRVFVRLNQLSDEIILEVGDN
                     GSGISPGEVSNNSFGMKLVKTLVQQLGAGFQVENKQGTWFTIKMKNG"
     gene            1131..1724
                     /locus_tag="PK977_17070"
     CDS             1131..1724
                     /locus_tag="PK977_17070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HRF19892.1"
                     /translation="MKRELNSILLAEDELITASAIEELLLEEDYYVLGIAADGATALR
                     LCQQAPEPPALVICDINIKGSMNGITLAGELKQRYNCEIVFLTAYTDTKTLDSAFAQD
                     PVMYVVKPYSDKQLRAAVQMAFHRIYQKQKTIPPLKLQLTKREKEIATLVAQGLSSKQ
                     IAQQLHISEETVKTHRRRMLQKNNISSFAHLVYLMNR"
     assembly_gap    2213..2316
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2317..>2643
                     /locus_tag="PK977_17075"
     CDS             <2317..>2643
                     /locus_tag="PK977_17075"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF19893.1"
                     /translation="YIIGEFRASADFDPGAGTVNLTSAGGSDIFFAKYDASGNYVYAK
                     RLGGTAEDVGFGIAVDGSGNVYVTGYFIGTGDFDPGAGTANLISSGIQDIFFAKYDAS
                     GNYVYA"
     assembly_gap    2644..2693
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2694..>3292
                     /locus_tag="PK977_17080"
     CDS             <2694..>3292
                     /locus_tag="PK977_17080"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF19894.1"
                     /translation="VDGSGNVCVTGVFQGTVDFDPGAGTVSLTSAGSYDVLFAKYDAD
                     GNYVYAKALGGTGSDFGNGIAVDGSGNVYVTGYFSGTADFDPGAGTANIISAGTQDIF
                     FAKYDASGNYVYAKALGGTGGDYSNDIALDGSGNVYVTGYFNGTGDFDPGAGTANLTS
                     AGNNDLFFAKYDASGNYVYAKAVGGTGYDIGYRIVVDGGG"
     assembly_gap    3293..3310
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3311..>3540
                     /locus_tag="PK977_17085"
     CDS             <3311..>3540
                     /locus_tag="PK977_17085"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF19895.1"
                     /translation="TGYFDGTVDFDPGAGTATWTSAGASSDIFFAKYDASGNYVYARG
                     LGGTSTDIGNGIAVDGSGNVYVVGEFQGTVDF"
BASE COUNT          941 a          675 c          783 g          929 t
ORIGIN      
        1 aggctactgc ctttaatttt tttctgggcc tgcatatcat tacaatctgg ttaggtattg
       61 ggggaatggc cgcatcattg gtttgttttt atatatcaag agtaaaaaaa caattctccc
      121 ccgttttaat tttcacttat gcaattattg caattctgct tctttcccca ctgtacttct
      181 ataatggagg atcaaacggg cctgtttttt tcctgatcat catgctgctt tttatataca
      241 tttttatagc gccttctttt atgcaaggct ggatttatat aatatatagc attgccnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttt acacggattg ggtagtgccg
      361 tatgattcaa aagagcagcg ggttatcgac ttttcaattg tactggtata tattactttt
      421 tttacttcat ttaccatctg gattttccgg aaaagttatg ataaggagca acggactatc
      481 cggttacaaa aaggagaact ggaaaaatta taccagcagg ccaatgaaaa aaaccagtac
      541 atcgaatcgc tgatcaaaga actgcaccat cgggttaaga ataacctgca ggtagtaagc
      601 agccttatgt ctttgcaatc aaacagaatg aaggctgatg aggcccgcaa cgcactggaa
      661 gaaggccgta accgtgtaaa tgccatggcg ctgattcacc agaaattata tatggatgat
      721 gaactgtcat cggttaacat gaatgaatac ctcgaaaatc tatcggcttc tcttgcagaa
      781 agcttcgggt atccacctgc tgttgtttca ccacaggtac aacttacagg ggataagctg
      841 gatatagaca gggcggtgtc tattggcctt attgtaaacg aactggtaac caattccttt
      901 aaacatgcgt ttcacggcca tacgaatcca agagtttttg ttcgcttaaa tcaattgtcg
      961 gatgaaataa tactggaagt cggagacaac ggtagtggca tcagcccggg ggaagtatcc
     1021 aataattctt ttgggatgaa actggtaaaa acactggtgc agcaactcgg cgccggcttt
     1081 caggtggaaa ataaacaagg tacctggttt acaatcaaaa tgaaaaacgg gtgaagagag
     1141 aactaaactc cattcttcta gcagaggatg aattgattac cgcatcagca atagaagaat
     1201 tattgctgga ggaggattac tatgtgctgg gcatagcagc cgatggggcc acagcactac
     1261 ggctttgcca acaggctccg gaaccaccgg cccttgttat ttgtgatatc aacatcaaag
     1321 gaagcatgaa cggcattaca ctggcaggcg aattaaaaca gcgatacaac tgcgaaattg
     1381 tattccttac tgcttatact gatacaaaaa ccctggattc ggcttttgca caggatccgg
     1441 tgatgtatgt ggtaaagccc tacagcgaca agcaactcag ggctgccgtt caaatggcct
     1501 ttcaccggat ttaccagaag caaaaaacca taccgccttt aaagctgcaa ctcacgaaac
     1561 gggaaaaaga gattgccacc ctggtggcgc agggactctc ctctaaacag atagcccaac
     1621 agttacacat cagcgaagaa accgtaaaaa cccaccgccg ccgtatgctg caaaaaaaca
     1681 atatcagcag ctttgcccat cttgtttacc tgatgaaccg gtaagcaatc ctttatttca
     1741 acaaaccaat atttcttgta ccgtttttgg ggtataggca gcaactcgtt ttataatgaa
     1801 attgcaaagc tgatgaccaa cgtttactta ttataaggta aatcagccgg aacatacgga
     1861 tgtatcattt tatttttttt ggaaaataat ttattgatca cttatgagaa ttaatctccc
     1921 aacacctgta ttatcattac tttttaattt aatttttgtt aaaggatata ctcagcaaat
     1981 caccataagt ggacctgccg gcagtgggtc ctttggtaat gtaacattct taccaatggc
     2041 aattatataa ttaccgttcc ctatttgatg aaggcgctgt ttaataactg cggccaagtt
     2101 tttaaccata gaatatatta cccggtataa aatgaaatag tatgaggatt attttacaat
     2161 ttgtattagc agttatctgt ctttctttaa tggcttcagc cggcgcccag gannnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatac attatcggag agtttagggc
     2341 ctctgctgat tttgacccag gtgccggcac ggtcaaccta accagtgcgg gaggcagtga
     2401 tatctttttt gccaaatacg atgccagtgg caactatgtc tatgctaaaa gactgggcgg
     2461 aacagccgag gatgttggct ttggtatagc agtagatggg agcggcaatg tatacgttac
     2521 gggctatttc atcggaacag gggactttga cccgggtgcc ggcacggcga atctcattag
     2581 ttcaggaatt caggatatat tttttgccaa atacgatgcc agtggcaact atgtttatgc
     2641 aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcgatg
     2701 gcagcggtaa tgtatgtgtt accggtgttt tccaaggcac agtggatttt gatcccggtg
     2761 ctggtacggt tagccttacc agtgcgggaa gttatgatgt cttatttgcc aaatatgatg
     2821 ctgacggcaa ctatgtatat gccaaagccc tgggcggtac aggcagtgat tttggcaatg
     2881 gtatagcggt cgatggcagc ggcaatgtat atgtcaccgg ttattttagt ggtacagcgg
     2941 attttgaccc cggcgccggt acggcgaaca ttattagtgc aggaactcag gatatctttt
     3001 ttgccaaata cgatgccagc ggcaattatg tatatgccaa agccctgggc ggaacaggcg
     3061 gtgattatag caatgatata gcgttagatg gcagcggcaa tgtatatgtt acaggctatt
     3121 tcaatggaac cggggatttc gacccgggtg caggcacggc caaccttacc agtgcaggaa
     3181 acaatgattt attttttgcc aaatacgatg ccagtggcaa ctatgtctat gccaaagccg
     3241 tgggcggaac aggctatgat attggctatc gcatagtcgt agatggcggc ggnnnnnnnn
     3301 nnnnnnnnnn tacaggctat ttcgatggaa cagtggattt tgaccccggt gccggcacgg
     3361 ccacctggac cagtgcagga gcaagttccg atatcttttt tgccaaatac gatgccagtg
     3421 gcaactatgt ttatgccaga gggctgggcg gaacaagcac tgatattggc aatggtatag
     3481 cggtagatgg cagcggtaat gtgtatgttg tgggcgagtt ccagggcact gtagattttg
//
LOCUS       DAORLS010000309         3533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14437_length_3533_cov_2.035078, whole genome shotgun sequence.
ACCESSION   DAORLS010000309 DAORLS010000000
VERSION     DAORLS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_3533_cov_2.035078"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK977_17090"
     CDS             complement(<1..607)
                     /locus_tag="PK977_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF19896.1"
                     /translation="MKKILFTAIAALSLVISDAQLRTPAPSPSQTIKQDFGLSSVELS
                     YSRPAMKGRKIFGDLVPFGKVWRTGANNATTLTFGEEVTIGGTKVPAGKYGLLTIPDK
                     KSWTIIISKQTDVTSPAAYKQDQDVVRVEAKTMEMNSTMESFTIQFADVKPTSCELHI
                     MWDKTAVALPITVDVEKKVMAQIDQLMNKDNRPYFNAAMYYM"
     gene            complement(720..>1454)
                     /locus_tag="PK977_17095"
     CDS             complement(720..>1454)
                     /locus_tag="PK977_17095"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF19897.1"
                     /translation="KAKAAGQVRQESDADGLKDAAAKKQTDETRNNRAIAANRKAEEQ
                     NYRNQQSNVFRGRVTDQNNVGVPFANVTNLDDNEAGTYTDARGNFILTYPDSVLNVQV
                     RSIGFEYNNVQLRNSVPTNQVILQDDRSLSEVVISNKKPNAVARSRETNMKLEEPEPA
                     DGWDNYDSYLVNNVKLPDEYVTKQQGGGEVQVSFEVDKNGEPVNFKIEKSLCKKCDQE
                     AIRLIKEGPKWRRKAKNGRTTVTITF"
     assembly_gap    1455..1688
                     /estimated_length=234
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1689..2150)
                     /locus_tag="PK977_17100"
     CDS             complement(<1689..2150)
                     /locus_tag="PK977_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19898.1"
                     /translation="MYCRMATNNNIKNFTAADIEKYHKGQLSGKERHDLEKAALDDPF
                     LADALEGYAISGVNAGADIAELKQRLSEKLEEKTKIIPISNGRNNFRLLRAAVVIAFV
                     AGAGLLVYQFGFNNKKEEIVQAEPVKKVETTTKNNDVATGSDGTLADQDKTK"
     gene            complement(2165..>2647)
                     /locus_tag="PK977_17105"
     CDS             complement(2165..>2647)
                     /locus_tag="PK977_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF19899.1"
                     /translation="MELVYGVCLKYYKEPETAKDSVMQIFEELVLKLKKHEVDNFRGW
                     LHQVAKNHCLMQLRTPKNMKTVEFKTEIVQSEEEVHLNGVLEKEENFKKLEFCLGTLT
                     DDQREAVKLFYLDGKCYNEIVEITGKDWNQVRSFIQNGRRNLKLCMEKSAAEAGRRAE
                     "
     assembly_gap    2648..2659
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2853..>3138
                     /locus_tag="PK977_17110"
     CDS             2853..>3138
                     /locus_tag="PK977_17110"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF19900.1"
                     /translation="MKRILVMIVPVVLILLAFRPVKSHTVTGKVTDETGAALAGVSVH
                     VKGTSTGVSSDVNGSFRITAPGSNSTLVFSAAGYVTAEIKINGRTSISITL"
     assembly_gap    3139..3154
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3201..>3533
                     /locus_tag="PK977_17115"
     CDS             3201..>3533
                     /locus_tag="PK977_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HRF19901.1"
                     /translation="MAQKQRKAVTGSVSTLYGAPPSSAYYQPVYDQIQQYRTDKDGIK
                     EDFNREGYDKITENRFLKVTDNPLSTFSIDVDAASYSNIRRFLNQGQLPPAGAVRIEE
                     MVNYFTYQY"
BASE COUNT          809 a          728 c          672 g         1062 t
ORIGIN      
        1 ccatatagta catcgctgca ttgaagtaag gacggttgtc tttattcatc agctgatcta
       61 tttgtgccat tactttcttt tccacatcta cagtaatagg cagtgctacg gctgttttat
      121 cccacattat gtggagctcg cagctggtag gttttacgtc agcaaattga atggtaaatg
      181 attccatagt actgttcatc tccattgtct tcgcttctac tctaaccaca tcctggtctt
      241 gtttgtaagc tgcagggctg gttacatctg tctgttttga aatgatgatt gtccagcttt
      301 ttttatcagg aatagttaaa agaccatatt tacccgctgg cactttggtg ccgccgatgg
      361 taacttcttc gccaaaagtg agggtagtgg cattattggc ccctgtacgc catactttgc
      421 caaaaggaac caggtctccg aaaatctttc tgcccttcat tgccggacgg gagtaggata
      481 actctacgga agaaaggcca aagtcctgct taatagtttg agacgggctg ggagccggtg
      541 ttcttaattg tgcatcacta attactaagg ataatgccgc aatagcggtg aaaagtattt
      601 tcttcatact gcaagttttt gagaaggcaa aggtagggga aaggaagttg tcctgagact
      661 ggaaccaaga gtcgtgagtc agaagtctga atccttagat gaggcctaat tcttttactt
      721 taaaaagtga tcgttactgt agtacgtcca tttttggctt tccgcctcca tttcggacct
      781 tctttgataa gccggatggc ttcctggtca cattttttac agagtgattt ttcaatcttg
      841 aaatttacag gctcaccatt tttatctact tcaaaggaaa cctgcacttc gccgccaccc
      901 tgttgtttag ttacatattc atccggaagc ttgacattat tgacaaggta ggaatcatag
      961 ttatcccatc catctgcggg ttcgggttct tctaatttca tattggtctc tcttgaacgg
     1021 gccacagcat tcggcttttt attgctgatt accacttccg aaaggctccg gtcgtcctgt
     1081 aatattacct ggttagtcgg cacactattt ctgagctgta cattgttgta ctcaaaacct
     1141 attgaacgaa cctgcacatt tagtactgaa tccggataag tgagaatgaa gttgcccctg
     1201 gcatctgtat aggttcctgc ttcattgtca tccaggttgg ttacattggc aaacggtacc
     1261 ccgacattat tctggtctgt aacacggcca cgaaagacat tcgattgctg gttacggtaa
     1321 ttttgttctt ccgctttacg gttagcagca atagccctgt tattcctagt ttcatctgtt
     1381 tgctttttgg cggctgcatc ttttagcccg tcggcatcag attcctgtct tacctggccg
     1441 gccgcttttg ctttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnntt tagttttgtc ttggtcggca agtgtaccat ctgatcccgt cgcaacatcg
     1741 ttgttttttg ttgtggtctc tactttttta acaggctctg cctgtacgat ttcttctttt
     1801 ttattattaa aaccaaactg gtaaaccaat aatccggctc ctgcaacaaa agcaatgaca
     1861 actgccgccc ttaataaacg gaaattattc cgaccattac tgataggaat gattttagtc
     1921 ttttcttcaa gtttttctga cagccgttgt ttaagctcgg ctatgtccgc ccctgcattt
     1981 accccggaaa tagcatagcc ttccagcgca tctgccagga agggatcatc taaagctgct
     2041 ttttccaggt catgcctttc tttgcccgaa agctgacctt tgtgatattt ttcaatatct
     2101 gcggcggtaa agttttttat gttattattc gttgccatac ggcagtacaa agttcttatt
     2161 tttatcattc agctcgcctg cctgcttctg ctgcggattt ttccatacag agcttcaggt
     2221 ttcggcggcc gttctggata aaacttctga cctggttcca gtctttcccg gttatttcca
     2281 caatttcatt ataacacttt ccatccaggt aaaacaattt tacggcttcc cgctggtcat
     2341 ctgtcagtgt gccgaggcaa aattccagct ttttgaaatt ttcctctttt tccagcacac
     2401 cattcagatg cacttcttct tcagattgca caatctctgt tttaaactct acagttttca
     2461 tgttttttgg ggttcgcagt tgcatcaggc aatggttttt ggctacctgg tgcaaccagc
     2521 ccctgaaatt gtctacctca tgttttttta attttaatac cagttcttca aaaatctgca
     2581 tgacactgtc tttggcagtt tcaggctcct tataatattt aaggcatacg ccatatacca
     2641 gctccatnnn nnnnnnnnng taccgctggt atagttctcc cagtacggcc atgtcacccg
     2701 attgcttata cagagccacc agctccttgt cggttagtga gttgtgagat atgttgcgga
     2761 tgaaagccaa cggctaaaat tagcgaataa aatttttata actgctttat gcaattgtca
     2821 aaataccggc atcagggtat taaaattatt ttatgaagcg aatacttgta atgatagtgc
     2881 cggtagtatt aatattactg gctttccgcc cggtgaaatc tcatacagta accggtaaag
     2941 taactgacga aaccggcgct gctctggccg gtgtatctgt tcatgtaaaa ggaaccagta
     3001 ctggtgtaag ttcagatgtg aacggatctt tcagaattac ggctcccggt tccaattcca
     3061 ccctggtctt ttcagcagcc ggttatgtaa ctgctgagat taaaataaat ggtcgtacca
     3121 gtatatccat tacacttann nnnnnnnnnn nnnnacttaa agccgcttcg cagactatgc
     3181 aggaagtggt tgtggttggg atggcgcaaa agcaaaggaa agcggtgaca ggcagtgttt
     3241 ctacattgta tggtgcacca ccatcaagcg cctattatca accagtatat gatcagatac
     3301 aacaataccg tactgataaa gacggtatca aagaagattt taaccgtgaa ggttatgata
     3361 aaataactga aaaccggttt ttgaaggtaa cggacaaccc actttctaca ttctctattg
     3421 atgtagatgc agcttcatac agtaatatcc gccgtttcct gaaccagggg caattgccac
     3481 cggcaggagc tgtacgtata gaagaaatgg ttaactactt tacttatcag tat
//
LOCUS       DAORLS010000310         3523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14499_length_3523_cov_1.561995, whole genome shotgun sequence.
ACCESSION   DAORLS010000310 DAORLS010000000
VERSION     DAORLS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14499_length_3523_cov_1.561995"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PK977_17120"
     CDS             <1..226
                     /locus_tag="PK977_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19902.1"
                     /translation="YDVENGLFNNFTKNNPVSYRMERYGSLQTGIDTKQVSIEYTYNS
                     SGFPITIKANQNGVPYLPPNTVSYFYYSYL"
     assembly_gap    438..447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <448..>948
                     /locus_tag="PK977_17125"
     CDS             <448..>948
                     /locus_tag="PK977_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRF19903.1"
                     /translation="IVVLSALSGTTNTLVEIGELIAKGDRSGAKQAIDKLEAHYLVFV
                     TELVKKEEAVAKAKAIIAEHFEFLNIILKISFSEALSKDILAQGELLSTKLFCAYLEE
                     QGIDHLLLPALEFMTIDSYDEPQIGSIKVKLTQILQQHKDKKLFVTQGYICRNARGEV
                     DNLKRGG"
     assembly_gap    949..1067
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1068..1680
                     /locus_tag="PK977_17130"
     CDS             <1068..1680
                     /locus_tag="PK977_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRF19904.1"
                     /translation="IEQMSFDEAAELAYFGAKILHPASIWPAQTYKIPVRLLNTMQPE
                     AKGTLITEEAGSVGVKAVAAKDNIIAIRIKSSRMLLAYGFLRKIFEVFEKYRTPIDMI
                     TTSEVAVSLTIDNDANLKEILKELEPFGTVEVDKNQTIISVVGNEISRTEDMVKKLFG
                     SIMNIPVRMVSYGGSPHNISLLIAADYKTKMLQQLNKGMFGLD"
     gene            complement(1752..2219)
                     /locus_tag="PK977_17135"
     CDS             complement(1752..2219)
                     /locus_tag="PK977_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19905.1"
                     /translation="MKRLVIISFLGLFSLSVSSQSYKGVNRPFNLSQELNDLYCSGLF
                     HSTEGTIIDVANNMGARAWLNILDWLEGRVAGLQVYKSKSGARIPVIRGGVPGIYVDE
                     MSVPIDYLNSLNIADIAIIKVIKTPFFGGFNGGNGAIAIYTLGSEEEDSEHLD"
     gene            complement(2336..>2800)
                     /locus_tag="PK977_17140"
     CDS             complement(2336..>2800)
                     /locus_tag="PK977_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRF19906.1"
                     /translation="LIKRLEIKGIRFAEVTLHTGLGTFRPIEVEDLSKHKMDAEYYHI
                     DETACKIVNRAKEYGHRICSIGTTTMRALESSFTAQKLLKPSEGWTNMFIHPPYEFNI
                     ADSLVTNFHLPKTSLLIMTCAFAGYELAMEAYKKAIKDKYRFFSYGDALLII"
     assembly_gap    2801..2883
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2884..>3150)
                     /locus_tag="PK977_17145"
     CDS             complement(<2884..>3150)
                     /locus_tag="PK977_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRF19907.1"
                     /translation="VFLLRELNKPNRLWDVIVDPARKIRVGNKLYFGDQEDLVAEVID
                     NTTSRGRTIRFLWDGSEEDFRAQLEKLGETPLPKYIKRKPDEAD"
     assembly_gap    3151..3272
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3273..3398)
                     /locus_tag="PK977_17150"
     CDS             complement(<3273..3398)
                     /locus_tag="PK977_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRF19908.1"
                     /translation="MKLSQFRFDLPLNLIAQTPAKRREDARMMVVNRHTGEIENKH"
BASE COUNT          961 a          604 c          717 g          907 t
ORIGIN      
        1 atatgatgta gaaaatgggt tgtttaataa ctttacaaaa aataatcctg tgagttatcg
       61 gatggaaagg tatggaagtt tgcaaacggg aattgatact aagcaagtaa gtattgaata
      121 tacatacaat agttcggggt ttccgataac aataaaagct aatcaaaatg gggtgcccta
      181 tttaccacca aatactgtga gttactttta ttattcgtat ctctgaaata ttttagaatg
      241 acaactttaa cgactatgta attttatcta atagtctatc tttgcgccct tgcttgcctt
      301 ttcttaatca attaaaatct cgggaggggg ggacaaacat gaaagtcatg aagtttggcg
      361 gcaccagtgt gggtaagccg gaacgtatgc atcacattgt aaagctggta actaaggagg
      421 cggaaattga accggctnnn nnnnnnnatc gttgtattat cagcattgtc tggcacaacg
      481 aataccctgg tagaaatagg ggaactgata gctaaaggcg accgcagcgg cgccaagcaa
      541 gcaatagaca aactggaggc acattacctg gtatttgtca cagaactggt aaaaaaagaa
      601 gaagctgttg ctaaggcaaa agcaattatc gccgagcatt ttgaattcct gaatattatt
      661 ctcaaaatat cgtttagcga ggcattgagt aaagacatac tggcgcaggg cgaattgctc
      721 tctactaaat tattctgtgc ctacctggaa gaacagggta ttgatcacct gttgttaccc
      781 gctttggagt ttatgaccat tgatagttat gatgagccac agattggcag tattaaagtg
      841 aagcttacgc aaattttaca gcagcataag gataaaaaac tattcgttac acagggctat
      901 atctgtcgga atgcaagggg cgaagtggat aacctgaaaa gaggaggann nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat agagcaaatg
     1081 agttttgatg aggcggcaga gctggcttat ttcggggcta agatccttca cccggcttct
     1141 atatggccgg cacaaacgta taaaataccg gtgagattac tgaataccat gcagccggaa
     1201 gcaaagggaa cgcttataac agaagaggcg ggcagtgtgg gagtaaaagc agtggcggca
     1261 aaagataata tcattgctat ccgtatcaag agcagccgga tgttgctggc ttatggtttc
     1321 ctgcggaaaa tttttgaagt atttgaaaaa taccgcacac ccatcgatat gatcactact
     1381 tcggaggtag ctgtttcatt aacaattgac aatgatgcaa acctgaaaga gatattaaaa
     1441 gaactggaac cattcggtac agtggaagtg gataagaacc agacgatcat ttctgttgta
     1501 ggtaatgaaa tatctcggac agaagacatg gtaaaaaaac tttttggctc aattatgaat
     1561 atccctgtcc gtatggtaag ctatggtggc agtccccata atatttcttt gcttattgcg
     1621 gctgattata aaacaaaaat gttgcagcaa ttaaataaag ggatgtttgg actggattaa
     1681 atttagtaat gctaaaagaa agtctggtag ctatataccg gcttttttaa taaggttaaa
     1741 aaggtatgtg attaatccag atgttctgaa tcttcttctt cgctgcccaa cgtatatata
     1801 gcgattgctc cattacctcc gttaaatccg ccaaagaagg gagttttaat gacttttatt
     1861 atcgcaatgt ctgctatatt taatgaatta aggtagtcga tgggaacaga catttcatct
     1921 acatatatac cgggaacgcc cccgcggatg accggtatcc tggctcctga ttttgattta
     1981 tacacctgta gtccggcaac ccgtccctcc aaccagtcaa ggatattaag ccaggccctg
     2041 gcacccatat tattggctac atcaataatg gttccttcgg tactatggaa caagccgctg
     2101 caataaagat cgtttagttc ctgtgagagg ttaaaaggac gatttactcc tttgtatgat
     2161 tgtgaagaaa cagagagtga gaataaacct aaaaaagaaa ttattactag tcgtttcata
     2221 accacatcct cctgtgtgta agttacgacg aattgctgtt aggagatagt taagagaatt
     2281 aaaaaagcct tctaaagaag gctcttagtg tataattaag tattaaaaaa tgcctttaaa
     2341 tgatcaacag tgcatcacca tagctgaaga acctgtattt gtccttaatc gcttttttat
     2401 aagcttccat ggccagttca tagcccgcaa aagcacaggt catgatcagc agacttgttt
     2461 taggcaagtg aaaattggta accagtgagt ctgctatatt aaactcatag ggggggtgaa
     2521 tgaacatatt ggtccagccc tccgaaggtt tcaataattt ctgagcagta aaagaggatt
     2581 ccagtgcccg cattgtcgta gtaccaatgg agcagatacg gtgaccatac tctttagccc
     2641 tgttcacaat tttacaagct gtttcgtcaa tatggtaata ttctgcatcc attttatgct
     2701 tgctcaggtc ttccacttca atgggacgga aagtaccaag ccctgtatgc agtgtcactt
     2761 ctgcgaaacg gataccctta atctccagtc gtttgatcaa nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnttatccg cttcatctgg tttgcgtttg atgtatttgg gtaaaggagt ttcgcccaac
     2941 ttttccaatt gtgccctgaa gtcttcctcg ctaccatccc agagaaaacg gatggtacgg
     3001 ccacggctgg tagtattgtc aattacttca gctaccaggt cttcctggtc gccaaaataa
     3061 agtttgttgc caacacggat cttccttgcg ggatcaacta tcacatccca aagacgattg
     3121 ggtttgttca gttcacggag tagaaatact nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgtttgt tttcgatttc accggtatga
     3301 cggttaacca ccatcatgcg ggcatcttca cgtcttttgg ccggagtttg ggcgataagg
     3361 tttaacggga ggtcgaaacg aaattgcgaa agcttcatgt gtatttaggg tttatatcaa
     3421 tagtcgttga cacaaaccgg gcatgaatat ttgccggggg tgatcctaaa tccggcctta
     3481 cggcgccggg tttggtcaat tccgaattaa atttaggcgg gca
//
LOCUS       DAORLS010000311         3517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14524_length_3517_cov_1.822935, whole genome shotgun sequence.
ACCESSION   DAORLS010000311 DAORLS010000000
VERSION     DAORLS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14524_length_3517_cov_1.822935"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..>1736)
                     /locus_tag="PK977_17155"
     CDS             complement(183..>1736)
                     /locus_tag="PK977_17155"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRF19909.1"
                     /translation="IMSRYGDTPMGMVESAMEFLRIARAEAYHNIILSMKSSNPQVMV
                     QAYRLLVKTMTDEFGECYPLHLGVTEAGDGEDGRIKSAIGIGTLLEDGIGDTIRVSLT
                     EDPEFEIPVCRDIVKRYSKRESREVQTEYQTNGDKQVVIQHVNITEELDAKDVPTVTK
                     IPYSPFEYKRREAYAVDNIGGKQVPVVVADLSRIDKITPRDLQSVGYTYNESTDKWTI
                     ADAAADYIFTGHQVLDFALPGTLKVIVYPAAWTEATDKKKYYPIFSDKGFAEAEKKSE
                     SINFVMVDCYSDETAVNDYAYLDQLANDPTVVICLSSTRLNAMQSVRRMFVEFMNKGI
                     KNPVVLITDSNWQSPDEHLIHFATETGALFLEGMGDGICLGYSNKAKMTDVKVTGRTY
                     LPVKDIYQFTNNTAFSILQATRTRISKTEYISCPSCGRTLFDLQETTAKIRAVTNHLK
                     GIKIAIMGCIVNGPGEMADADFGYVGSGPGKITLYKGKEVVKRNVNSDVAVEELIGLL
                     KDNGAWVEP"
     assembly_gap    1737..1872
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1873..>2129)
                     /locus_tag="PK977_17160"
     CDS             complement(<1873..>2129)
                     /locus_tag="PK977_17160"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRF19910.1"
                     /translation="IGTVEQSIRCIEAGAELVRITAPSKKEAENLLVIKNELRKRGYN
                     TPLIADIHFTPNAAEIAARIVEKVRVNPGNYVDKKKFEQIE"
     assembly_gap    2130..2150
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2318..>2788)
                     /locus_tag="PK977_17165"
     CDS             complement(2318..>2788)
                     /locus_tag="PK977_17165"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF19911.1"
                     /translation="DPSSGTAKFNKANSVYKQDKKVEASEQFTAIARSVTDKDIKAKS
                     FYNKGVILSQQKNLEESIEAYKNALRINPADKEARENLQKALLELKKKQPPKKENEQQ
                     KKKQQEQQKKQQQPKMSQKEAEQRLKLLQQKEKEVQQKMQKEKSKSGGSQPKDW"
     assembly_gap    2789..2838
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2938..>3517)
                     /locus_tag="PK977_17170"
     CDS             complement(2938..>3517)
                     /locus_tag="PK977_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF19912.1"
                     /translation="LQMPLTADHGAARLFVSGADPAIIQQQGTVISDALQMSANAFVS
                     AERKFKAVILISDGEDHDAGAVSTAKELSEEGVMINTVGIGSPEGSAIIDPATGDNKK
                     DAEGNIVISKLNEEVLKELAEVTNGIYARLQSSDEAVKVLKAQLSQIDRKAFGDMSLM
                     NFTTYYYVFAGLMFLLLLVEPFIPEKKRGYAK"
BASE COUNT          857 a          776 c          587 g         1090 t
ORIGIN      
        1 gttttgaaat ggtctgtgcc caataatgcg ggataacagc accggcaata ttcaaagcca
       61 gcatcaagag atatatcaaa atggaaatac catttttcat gtgtaatgag aattgaaata
      121 aatcaggatt ccaaaaacaa tattataatt ctgcaatcta aattctaaaa tccaaaattg
      181 tactatggtt ccacccaggc gccgttatct tttagcaacc ctatcaattc ttctacagct
      241 acatcactat ttacatttcg ttttactact tccttgcctt tatacagggt gatcttgccg
      301 gggccgctac caacataacc aaaatccgca tcggccattt cgcccgggcc atttacaata
      361 caacccataa tagcaatctt gatgcctttc aaatgattgg taaccgccct gatctttgct
      421 gtagtttcct gcaaatcaaa taaagtgcgg ccacaactgg gacagctgat atattctgtt
      481 ttcgaaatcc ttgttctggt cgcctgtaaa atgctgaatg cggtgttgtt ggtaaactgg
      541 taaatatctt ttaccggtaa ataagttctt cctgtaactt ttacatccgt catcttggct
      601 ttgttactat agcccaaaca aataccgtct cccataccct ccaggaacaa tgcacctgtt
      661 tcggtggcaa aatgtatcag gtgttcgtcc ggtgattgcc agttactgtc agtgattaaa
      721 acaaccggat tcttaatccc cttattcatg aattccacaa acatccgccg aacagactgc
      781 atggcattca aacgggttga gctaagacaa ataacaacgg tcggatcgtt tgctaattga
      841 tctaaataag cataatcatt aacggctgtt tcatcactat aacaatcaac cattacaaag
      901 ttgattgact cgcttttctt ctctgcttct gcaaaccctt tatcagaaaa aatgggataa
      961 tacttctttt tgtcagtagc ttctgtccat gctgcgggat aaactatcac tttcaatgta
     1021 ccaggtaaag caaaatccag tacctggtga cccgtaaaaa tataatcagc tgcagcatca
     1081 gcaattgtcc atttatctgt tgattcattg taagtgtacc cgacactctg caaatcccgg
     1141 ggagttattt tgtcaatccg gctaagatct gcaaccacta ccggtacttg tttaccccct
     1201 atattatcca ctgcataggc ttctcttctt ttgtattcaa aaggagaata aggaatttta
     1261 gttacagtag gtacatcctt tgcatcgagt tcttctgtaa tgttgacatg ttgaataact
     1321 acctgcttat ctccgttagt ttgatactct gtttgtactt ccctgctctc tctcttagaa
     1381 tatcttttaa caatatcacg gcatacggga atttcaaact ccgggtcttc ggtaagtgat
     1441 accctgatag tatctccaat tccgtcctca agcaacgtac cgataccaat agcagattta
     1501 atacggccat cttcaccatc tcctgcttcc gtaacgccaa ggtgcaaagg ataacattcg
     1561 ccaaactcgt cagtcatggt tttcaccagc agccgatagg cctgcaccat cacctgcggg
     1621 ttgctgcttt tcatactaag tatgatatta tgataggctt ctgcccttgc aatccgcaaa
     1681 aattccatgg cgctttctac catacccatt ggggtatcac catagcggct catgatnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nntactcgat ctgctcaaat tttttcttat caacataatt accggggttc
     1921 accctcactt tctctacaat ccgggcagct atttctgccg cattaggtgt gaaatgaata
     1981 tctgcgatca gcggagtgtt gtaacctctt tttctcaatt catttttaat gaccagtaaa
     2041 ttttcggctt cttttttaga aggagcggtg atgcgtacca gttctgcacc tgcttcaata
     2101 caacggatgc tttgctctac ggtgccgatn nnnnnnnnnn nnnnnnnnnn gtgtccattg
     2161 tatcggtagt agtcatggtt tgtacccgaa taggattaaa gtttcccaat aacaggtcac
     2221 cgatttttac ctccttcgta agcagtcgct tataactggt gagagaatcg cagtagaatt
     2281 gcatagtaca aaactaacaa agaagcagca gctatgttta ccaatctttt ggctgggagc
     2341 cccctgattt tgacttttct ttttgcatct tctgttgtac ttctttttct ttctgctgca
     2401 ggagtttgag ccgttgttct gcttctttct gactcatttt cggctgttgc tgttttttct
     2461 gctgttcctg ctgctttttc ttttgctgtt cattttcctt tttgggcggt tgttttttct
     2521 tcagttccag cagggctttc tgcaggtttt cccttgcttc tttatcagca ggattaattc
     2581 tcaaagcatt tttgtaagct tcaatacttt cctccaggtt tttttgctgg ctgagaatga
     2641 ctcctttatt atagaaagat tttgctttta tgtctttatc tgttaccgac ctggcaattg
     2701 cggtgaattg ctcggatgcc tccacttttt tatcctgttt atatactgag ttcgccttgt
     2761 tgaattttgc agtaccactg gagggatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnntc ctgctttttg taagcctcat tacccgaacg gatatgatct
     2881 gccgactgct gtgattttgc agtaaaataa aaacaaagca atgccaataa aataaatcta
     2941 cttcgcatac cctctctttt tttccggtat aaacggctca accagtaaga gcaagaacat
     3001 aagtcccgca aaaacatagt aatatgtggt aaagttcatc agtgacatat caccaaatgc
     3061 cttacgatca atctgtgata actgtgcttt cagcactttg accgcctcat cactgctttg
     3121 caaacgggca taaataccat tggtaacttc agccagctct ttcaatactt cttcattcaa
     3181 tttagatatc acaatatttc cttctgcatc ttttttattg tcgcctgttg cgggatcaat
     3241 gattgcagaa ccttccggcg accctatacc cacagtattg atcattactc cttcttctga
     3301 cagttctttc gcagttgaca cagctccggc atcatgatct tctccatcag agatcagtat
     3361 cactgctttg aattttcttt ctgcagaaac aaaagcattg gcactcatct gtaaggcatc
     3421 gctgataacg gtaccctgtt gctgaataat cgccgggtca gcacctgata caaataatct
     3481 tgcagcacca tgatcagctg ttaacggcat ttgtaaa
//
LOCUS       DAORLS010000312         3510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14563_length_3510_cov_1.485673, whole genome shotgun sequence.
ACCESSION   DAORLS010000312 DAORLS010000000
VERSION     DAORLS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14563_length_3510_cov_1.485673"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PK977_17175"
     CDS             <1..450
                     /locus_tag="PK977_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRF19913.1"
                     /translation="NPELLIGQHVVSAPFVKQSGLEIMPTGYIVIDGGAPTTVSYISN
                     AAPVPADKNEIAMCTAMAGEMLGMKLIYMDAGSGAKRPITESMIKAVADSIEAPLIIG
                     GGIITPEKAYLNCKAGADVIVVGNAIEKDASLIKEMSDAVHSVPVKA"
     gene            complement(461..>697)
                     /locus_tag="PK977_17180"
     CDS             complement(461..>697)
                     /locus_tag="PK977_17180"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF19914.1"
                     /translation="KVKAEPDTDKMLYMRSDNYAFAQAGVPAHTIMSSDDDDRCYHKP
                     CDEYDRIDIAHLTKVIRGIAKAAEEIIKGEVKVK"
     assembly_gap    698..864
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<865..>1089)
                     /locus_tag="PK977_17185"
     CDS             complement(<865..>1089)
                     /locus_tag="PK977_17185"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF19915.1"
                     /translation="GRTKADEVLVFSAHYDHMGIINGNSKDSIMNGANDDASGTTAML
                     ALAEYFAMRGDNERTLLFCAFAGEEFGLVGS"
     assembly_gap    1090..1117
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1118..>1712)
                     /locus_tag="PK977_17190"
     CDS             complement(<1118..>1712)
                     /locus_tag="PK977_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19916.1"
                     /translation="SLLIQTLLPAQVPVDSILEKDVKAAIQFLASDELMGRANGSDGI
                     IKAATFIGEKFRAAGLQPLPGQAAYYIPFSLTTKNKKTPPAILEWNDGVLEPARFLYL
                     RQQPGHHAPLSLADFTVIELDNCITEDILSNYATQSGNFLLWTSKRQPDSSYFLPAVI
                     KIPAGGLQQDFLIVCADKQPDSLTLSAVPGYYSNTGYN"
     assembly_gap    1713..1841
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1842..3079)
                     /locus_tag="PK977_17195"
     CDS             complement(<1842..3079)
                     /locus_tag="PK977_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF19917.1"
                     /translation="MIKRLFTGLLAATTLFLLLTGCKSPGQAQQSGGINDDIINVKEV
                     ERIEKVLASDAMRGRRAFTPDIDKAADFIAAEFKTAGLGTLNNSGSYKQEFAMVRTKH
                     LSSSATLDGKVVDPKNILVITCQPQLKITEQSGYEKVVLKPGMTLQNEARKYARSNKN
                     LLVLVDKSYANSFGNLSRLRSTLFKTENTVVFVLAEAEPSSYVIESTHEITEQKLANV
                     VAVLPGKSLKNEYVVFSGHYDHLGVGKPVNGDSIYNGANDDAAGITGVIMLAKYFKQQ
                     NNNERTLIFAAFTAEEVGGFGSQYFSRQLNADKVVAMFNIEMIGTESKWGKNSAYITG
                     YEKTNMGAIMAKDLEGTGFTFHPDPYPAQQLFYRSDNATLARLGVPAHTISSSKMDVE
                     PHYHKLTDHVETLDMENMTA"
     assembly_gap    3095..3197
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3198..>3510)
                     /locus_tag="PK977_17200"
     CDS             complement(<3198..>3510)
                     /locus_tag="PK977_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF19918.1"
                     /translation="GEYTNNSILSENDQVFVKDGERCWFVKLSDIRLFESVGNYAKVF
                     FGPNKPLILKSLNALEERLDEKTFFRANRKHIVNLRMIEKIEPYFNGGLLLELKGGEK
                     IE"
BASE COUNT          810 a          738 c          605 g          930 t
ORIGIN      
        1 aatcctgaac tgctgatcgg gcaacatgtg gtatctgctc cctttgtaaa gcaaagtggt
       61 ttagagataa tgcctaccgg ctatattgta attgacggcg gtgctcccac taccgtttct
      121 tacatcagca atgctgcccc ggtgccggca gacaagaatg aaatcgctat gtgtaccgct
      181 atggccggcg aaatgctggg catgaaactg atctatatgg atgcgggcag cggcgccaaa
      241 cgccccatta ccgaaagcat gataaaagca gtggccgaca gtattgaagc cccgctgatc
      301 atcggtggcg gtatcattac cccggaaaaa gcttacctga actgcaaggc aggtgctgat
      361 gtgatcgttg taggtaatgc tattgaaaaa gatgcttcac tgatcaaaga aatgagtgat
      421 gccgtgcata gtgtgccggt gaaagcgtaa aaaatcaaca tcactttact ttcacctccc
      481 ctttgataat ttcttctgct gcctttgcaa tcccccttat cactttagta agatgcgcaa
      541 tatcaattct gtcatattca tcacagggtt tgtgatagca tctatcatca tcatcactgc
      601 tcattattgt atgggcaggc actcctgcct gtgcaaaggc ataattgtcg ctgcgcatat
      661 acagcatttt atccgtatcg ggctcagctt taactttnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnntgatcc gaccagccca aattcttctc ccgcaaaagc
      901 acagaatagc agtgtccgtt cattatcgcc ccgcatcgca aaatattctg ccagtgccag
      961 catcgctgtc gtaccagaag cgtcgtcatt ggctccgttc ataatgctgt ctttgctgtt
     1021 gccatttata atacccatat gatcataatg ggctgagaat accaatacct catctgcttt
     1081 tgtcctgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt gtaacctgta ttgctataat
     1141 agccaggtac ggcactcagt gttaatgaat cgggttgctt atcggcacat acaataagga
     1201 aatcctgttg taaaccccct gccggtattt taataacagc cgggaggaaa tagctgctgt
     1261 ccggctgtct tttacttgtc cagagcaaaa aatttccgct ttgtgttgca taatttgaca
     1321 gaatatcttc ggtgatgcag ttatccagtt ctataaccgt aaaatcagcc aatgaaagcg
     1381 gagcatggtg accgggctgt tgccgcaggt acaggaacct ggccggttcc aatacaccat
     1441 cattccactc cagtatagcc ggaggtgttt tcttattttt agtcgtaagg ctgaaaggaa
     1501 tgtagtaagc tgcctgtccc ggcagtggct gcagccctgc cgccctgaat ttttctccga
     1561 taaaagtcgc tgctttgata ataccatctg acccgttggc tcgtcccatc aattcatccg
     1621 atgcaaggaa ctgaatagca gcctttacat ctttttccag gatagagtct accggcacct
     1681 gggccggtaa tagagtctga attaataaag aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natggctgtc atattttcca
     1861 tgtcaagtgt ttctacatga tctgtcagtt tatgataatg cggttccaca tccatcttgg
     1921 aactggaaat agtatgcgcc ggcactccca gtcttgccag tgtagcattg tcagaacggt
     1981 aaaacaattg ctgggccggg taaggatcgg ggtgaaaagt aaagccggta ccttccaggt
     2041 ctttggccat aatggccccc atattggttt tttcataccc ggtgatataa gctgaattct
     2101 taccccactt actctccgtg ccaatcattt caatattgaa catggctacc actttgtctg
     2161 cattcaactg gcgggaaaaa tactgtgatc caaatccccc cacttcttcc gcagtaaaag
     2221 ctgcaaatat caatgtacgt tcattgttgt tttgctgctt aaagtatttg gcaagcatga
     2281 taacgccagt tatgccggct gcatcatcat tagcaccatt ataaatagaa tcaccattta
     2341 ctggcttacc tacacccagg tgatcataat gcccggaaaa aaccacatac tcatttttca
     2401 ggcttttacc cggcagaaca gccactacat tagccagctt ctgttctgtt atttcatgag
     2461 tggattcaat tacataactg gacggttcag cctctgccag tacaaatacc actgtattct
     2521 ctgttttaaa caaggttgaa cgtagtcttg acaaatttcc aaagctgttg gcatagcttt
     2581 tatcaaccag caccaggaga tttttatttg atcgggcata ttttcttgct tcgttctgca
     2641 gggtcattcc tggctttagc accacttttt catacccaga ctgttcagta attttcaatt
     2701 gtggctgaca ggtaattacc aggatatttt tagggtccac tactttgcca tccaatgtag
     2761 ccgaactgga gagatgtttg gtacgaacca ttgcaaactc ctgtttatag ctgccactat
     2821 tattaagagt gccaaggcct gcagttttga attctgctgc tataaaatca gcggctttat
     2881 ctatgtccgg tgtaaatgct cttctgcccc gcatagcatc tgaagcaagt actttttcaa
     2941 tccgctcaac ttcttttaca ttaattatat cgtcattaat accacctgac tgttgtgcct
     3001 gtcccgggct tttacaccct gttaataata aaaaaagggt cgttgctgcc aacaaccctg
     3061 taaataatct tttaatcatt atccttgttt aattnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnttc aattttttca ccacccttca actccagcaa taagcctccg
     3241 ttaaaatatg gttcgatctt ttcaatcatc cgcaggttta caatatgctt acggttggcc
     3301 ctgaaaaacg ttttttcatc cagcctttct tccaatgcat tcaatgattt taaaataagc
     3361 ggcttattgg ggccaaagaa aactttggca taattgccta cactttcaaa taaacgtata
     3421 tcacttagct tcacaaacca gcatcgttca ccatctttta caaatacctg atcgttttct
     3481 gataaaatac tattattcgt gtattcacct
//
LOCUS       DAORLS010000313         3500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14622_length_3500_cov_1.762264, whole genome shotgun sequence.
ACCESSION   DAORLS010000313 DAORLS010000000
VERSION     DAORLS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14622_length_3500_cov_1.762264"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..>1360
                     /locus_tag="PK977_17205"
     CDS             38..>1360
                     /locus_tag="PK977_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19919.1"
                     /translation="MKNANQAFGYRVRTIAAIDNEDQRFEEADKFLKQIEEEFKKGYE
                     KGGTEIPKSMLRAHSILVDLRAGKIPADMMNARLEELNKLMEEEYKVDKSLAERLIGD
                     PFGKIKNYLIKKGYGPEAVQKIIAKIKGVFIENWRAYAPEYAQKSAMSAYEFANHVNQ
                     KLNNFNEHLAKTKTGKVLSSELLNHVDNAIALYDAFFSGKDLKQSAWNVSWTAGTIWA
                     QGRWPFLAIPLGIYNSIQTKSPAPFAMAVAFYLFPFAGQVYMVTNIIDRLGVSQVRDY
                     NFRNHLDRLAVMAITDGEGHVVRFKIPRAFNGLNGSSDSLETDPAIATDEPARTNGIK
                     SVFYDPAFMYCPDIKYFESLVPKKTDLFGLYETKHKNLMTLFAFDNDFVGYLVGIKKF
                     KEEKEAGNLEEIAASEIGGQRIKMLDSLEIVIEDRLWSAVFTAIESTKK"
     assembly_gap    1361..1473
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1474..>2308
                     /locus_tag="PK977_17210"
     CDS             <1474..>2308
                     /locus_tag="PK977_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19920.1"
                     /translation="KIRKQIQNDPLYKQALKDRFSAGTAYERVAIPTLERYIEIYRQI
                     LVIQSKIFDVWRPFGVDASKLQDDPMRLLLMGGLSGSPMLTTDAGKDLQIAIQCLEAH
                     TKRASDIRYDLATALGRPIKETEKEDKEHLRILGEYGFGFERLIDADASGRSPLFKDE
                     DGEIVNRMQVYRKAYKDYLEALKNSPGINVTLQIKGEKETTETVPVAMEVTILNEQKK
                     PVATPQGTTLQWYLLEQSTAKYIKVGEGNTFTHDAKQQGSFSYLIKLIKTVEGKTVDI
                     AK"
     assembly_gap    2309..2318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2319..>3113
                     /locus_tag="PK977_17215"
     CDS             <2319..>3113
                     /locus_tag="PK977_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19921.1"
                     /translation="DWWVNVKRFDATAPGKGILVKTPSVIKQYDIFDVTLDIPAALKG
                     RQIKCNWGYATIGKSNDCSGAKLQYNDEQSFTGKDGKKYLADSTTVWVSAEVLLPGKN
                     YGDVYQLNPKVKFQHMSINVKVTDIWEGNSGPNWFRVERKPVKNPPRIPGWSNDKKAI
                     STAEAYATVGLKDDDPMETINSLEDLKKYTEKEAKEQAFRQLEVKEVSVGDFKGYGLY
                     SAPKYSPGGWSDAGYRSAGAGASFSGYVMKGNKIFEVYWSVGAGGA"
     assembly_gap    3114..3283
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3284..>3500
                     /locus_tag="PK977_17220"
     CDS             <3284..>3500
                     /locus_tag="PK977_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19922.1"
                     /translation="IEPKVDTIQPGSKVTVNAVISNDKPDYGPYTYNWSGHVEGNSSS
                     KSVQLESDRPGKKTITVTVDGKTPPGTA"
BASE COUNT         1115 a          591 c          745 g          756 t
ORIGIN      
        1 tgtagatgac attgtacaaa agtgtaaaaa ggccatgttg aaaaatgcta accaggcttt
       61 cggataccgg gttagaacca ttgcagctat agacaatgaa gaccagcgat ttgaagaagc
      121 cgataaattc cttaaacaga ttgaagagga atttaaaaaa ggatacgaaa agggcggcac
      181 cgaaatacct aaatccatgc tgagggcaca ttctatcctg gtagatctgc gggcgggaaa
      241 aattccggcc gacatgatga atgcaaggct ggaggaactg aataaattaa tggaagaaga
      301 atataaggtt gataaatcac ttgcagaacg tctgatcggc gatccgtttg gcaagatcaa
      361 aaactacctt attaaaaaag gatatggccc tgaagcagta caaaaaatca ttgcaaagat
      421 caaaggcgtt ttcatagaaa actggagagc ttatgccccg gaatatgcac aaaaaagtgc
      481 catgtcagcc tatgaatttg ccaatcatgt aaaccagaaa ctaaataatt tcaacgaaca
      541 tcttgcaaaa acaaaaaccg gaaaagtttt atcaagtgaa cttttgaacc atgtggataa
      601 tgccattgca ttgtatgatg cctttttcag tggaaaagac ctgaaacaat ctgcctggaa
      661 tgttagctgg acagccggta ccatctgggc gcagggcaga tggcctttcc ttgctattcc
      721 gctgggcata tacaactcca ttcaaacaaa aagcccggct ccttttgcaa tggctgttgc
      781 attttacctg tttccctttg ccggacaagt ttacatggtt accaatatca ttgaccggtt
      841 gggagtaagc caggtaaggg attataattt ccggaatcac ctggacaggc tggcagtaat
      901 ggctattaca gacggagaag gccatgtggt aagatttaaa atacccagag cattcaatgg
      961 attaaacggt tcatcagatt cattggaaac tgatccagct attgccaccg atgaacctgc
     1021 aagaaccaac ggtatcaaat cggtttttta tgatcccgcc tttatgtact gcccggatat
     1081 aaaatatttt gaaagcctgg tacccaaaaa aactgacttg ttcgggttgt atgaaacaaa
     1141 gcataaaaac ctaatgacgt tgtttgcatt tgacaatgat tttgtcggct acctggtggg
     1201 aatcaaaaaa tttaaagaag aaaaagaagc gggtaatctg gaagaaatag cagccagtga
     1261 aattggcgga cagcgaatca aaatgctgga cagtcttgaa atcgttatcg aggatcgttt
     1321 atggtcagct gtttttacag caatagaatc caccaaaaaa nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaaaatc agaaaacaga ttcagaatga
     1501 tcctttatat aaacaagcct taaaggacag attttctgcc ggcactgcct atgaaagagt
     1561 ggcaatacct acattggaaa gatatataga aatataccgg cagatactgg taatacaatc
     1621 caaaatattt gacgtttgga ggccgtttgg tgtagatgca tccaaattgc aagatgatcc
     1681 aatgagattg ttactgatgg gtggcctcag cggatctccc atgctaacta ctgatgccgg
     1741 aaaggatctg caaatagcta ttcagtgtct agaagctcac acaaaaagag cctctgatat
     1801 aagatacgac ctcgcaactg ccctgggcag acccattaaa gaaaccgaaa aagaagacaa
     1861 agagcacctg cggatattgg gtgaatatgg ctttggattt gaaagactga ttgatgcgga
     1921 tgctagcgga agaagccctt tgtttaaaga tgaagatgga gagattgtta acagaatgca
     1981 ggtataccgc aaagcgtata aggattatct cgaggcatta aaaaatagtc cgggcataaa
     2041 tgtaacactg caaataaaag gagaaaaaga gactacagag acagtacctg tagcaatgga
     2101 agtgacaatt ttaaatgagc aaaaaaaacc ggtagcaact ccacaaggca ctaccctgca
     2161 atggtacctg cttgaacagt caactgccaa atatattaag gttggagaag gcaatacatt
     2221 cactcatgat gccaagcaac aaggaagctt tagctatctg attaagctga tcaaaacagt
     2281 agaagggaaa actgtagaca ttgcaaaann nnnnnnnnct gactggtggg tgaatgtgaa
     2341 gcgatttgat gccacagctc cgggtaaagg tatactggta aaaactccat ctgtgatcaa
     2401 acagtatgac atttttgatg tgacacttga tatacccgca gcattgaaag gccgacagat
     2461 aaaatgcaac tggggttatg caacgatcgg taaaagcaac gattgcagcg gagccaaatt
     2521 gcagtataat gatgaacaaa gtttcactgg caaagacgga aaaaaatacc tcgctgacag
     2581 tactaccgtt tgggtgtctg cagaagtatt gttaccgggt aaaaattatg gcgatgtata
     2641 ccagttgaac cccaaggtga aattccagca tatgagtatt aatgtgaagg tgactgacat
     2701 ttgggaaggc aattcaggcc ctaactggtt cagggtggaa agaaaaccag taaaaaatcc
     2761 gccaaggatt cccgggtggt caaatgataa aaaagcaata agcacagcag aagcttatgc
     2821 aaccgttggc ctgaaagatg atgacccgat ggaaacaatc aacagcctgg aagacctgaa
     2881 aaaatacacc gaaaaagaag ctaaggaaca agcattcaga cagcttgaag taaaagaagt
     2941 gtctgtaggc gattttaaag gctatgggtt atatagtgcc ccaaaatata gcccgggtgg
     3001 ttggagtgat gcaggctaca ggtctgccgg tgcaggtgcc agttttagcg gatatgtcat
     3061 gaaaggcaac aagatttttg aagtatactg gagcgttggt gctggtggag cttnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaattgaa cccaaagttg
     3301 atacaattca gcccggaagt aaagtgaccg taaatgctgt tataagcaac gataaacccg
     3361 actatggacc atatacatat aactggagtg ggcatgtaga aggaaattca agtagcaaat
     3421 ctgtacaact ggaatctgac agacctggta aaaaaacaat tactgttact gtagatggta
     3481 aaacacctcc gggtactgct
//
LOCUS       DAORLS010000314         3483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14716_length_3483_cov_1.542007, whole genome shotgun sequence.
ACCESSION   DAORLS010000314 DAORLS010000000
VERSION     DAORLS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14716_length_3483_cov_1.542007"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>925)
                     /locus_tag="PK977_17225"
     CDS             complement(<1..>925)
                     /locus_tag="PK977_17225"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF19923.1"
                     /translation="ITADYILASLWYKNNIIYHVHDELIFLDGNSFKVIKKIPFPNTG
                     GVRCFARDENETILVGTNKGIFRIDSNGKILFHWNKEKGLPDDCIYAIAIDRNSNLWC
                     SSNKGIFRINKEGNIFQLTKEDGLQENEFNTGVVAVAEDSEIFFGGMNGVSSFFPSAI
                     NSFEEEIKLLVTRIRVNYTDLENDSASWNIKKIKSGYDQNSLSFDFVAMANNNPAQYI
                     YQYRMLGLDKEWIQNTGMQTVRYSLPPGKYTLQLYASRSFDRDAKPMKEIQITILPPF
                     WKTWWFFTVLGIFFISLLTYGINQRNKRKYAK"
     assembly_gap    926..938
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<939..>1264)
                     /locus_tag="PK977_17230"
     CDS             complement(<939..>1264)
                     /locus_tag="PK977_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19924.1"
                     /translation="AGLFIFDTTQQMIRHIAKLPGRTTSFGVNTILKDQNGNYLLFVL
                     GEKNIWVLNANLNALTSYPVTFSDTPPYPIDYFGNAIYQDNEKAVVQSMYQLFRTNFT
                     DKKTTA"
     assembly_gap    1265..1313
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1314..>2332)
                     /locus_tag="PK977_17235"
     CDS             complement(<1314..>2332)
                     /locus_tag="PK977_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19925.1"
                     /translation="SSRAQLLNRYNTFSYNVNEGLLQSTIGDIEVDKNNFCWVTFPNG
                     IQQFDGNNFRTIKIQPGLPDDKYAKLFRCSNGDLLISHSQGISRYEIDKNSFIQVYKQ
                     PAGLQKPVYFLGEDDGIIYCYDEKATIIGIQRGNFKIVSSVNTGMPSHSTQSENIPGI
                     SENIIDHKFAFWMKETICLWDLQNKKLLYQSPPIHKRSQYFLRLLTGDKVVYTTHSQN
                     SALQCWDFVSGTNKQLPITGKFLDKDFSRFNTLRWQNKYIVSINNHMYETDSSFRVLK
                     SEFVNFQNQPVAGNLTVANIVEDYLGNLYLQTINGGIRKIIRNNYPIKYFGSLNKEEN
                     RVIGL"
     assembly_gap    2333..2360
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2695..2763
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2764..>3098
                     /locus_tag="PK977_17240"
     CDS             <2764..>3098
                     /locus_tag="PK977_17240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19926.1"
                     /translation="ELWKTDGTEANTVLVKDINSGVSSSNPQSYTEMGGVLFFIATTS
                     SNGTELWKTDGTEANTVLVKDINTGTASSSPANLKVINGVLYFIATSATSGRELWRSD
                     GTEAGTFLV"
     assembly_gap    3099..3162
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3163..>3483
                     /locus_tag="PK977_17245"
     CDS             <3163..>3483
                     /locus_tag="PK977_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19927.1"
                     /translation="FYFTADDNINGEELWVSDGTTAGTFLLKDIYPGFSPSYISYLTP
                     VGNTVFFQAEDDVNGLELWKSDGTPAGTVLVKDIEPGLDGSNPYYLINFNGTLFFTAT
                     TIANG"
BASE COUNT          940 a          622 c          648 g         1050 t
ORIGIN      
        1 tttagcatat ttccttttat ttcgctggtt gataccataa gtaagcaatg aaatgaaaaa
       61 tatacccaga accgtgaaaa accaccaggt tttccagaat gggggaagaa ttgtgatttg
      121 tatttctttc atcggtttgg catctctgtc aaaagaacgg gaagcatata gctgcaaagt
      181 atattttccg ggaggaagtg aataacgaac tgtttgcatc cctgtattct gtatccactc
      241 tttatctagt ccaagcattc tgtactggta aatgtattga gccgggttgt tattggccat
      301 tgcaacaaag tcaaaagaaa gtgaattctg gtcataacct gattttattt ttttaatatt
      361 ccagcttgcg gaatcatttt caagatcagt gtagtttacc cgtatgcggg tgactaatag
      421 tttaatttct tcttcaaaac tgttgatagc tgaaggaaaa aaactgctta cgccgttcat
      481 gccgccaaaa aagatctcac tatcttcagc gacagccact acacctgtgt tgaattcatt
      541 ctcttgtaaa ccatcctctt tggtgagttg gaaaatatta ccctctttgt taatcctgaa
      601 gattcctttg ttactgctgc accaaaggtt gctattcctg tcaatggcga ttgcatagat
      661 gcaatcatcc ggaagtcctt tttctttgtt ccagtgaaaa agtatttttc cattactatc
      721 gatcctgaag atccctttat tggtgcctac taaaattgtt tcattctcat ctctggcaaa
      781 acaccggaca cctcctgtat tggggaaggg tatctttttt atcactttga aagagttgcc
      841 atcaagaaag atcaattcat cgtgtacatg ataaataatg ttgtttttat accaaagact
      901 ggcaagtata taatcggcag taattnnnnn nnnnnnnntg ctgtggtttt tttatcggta
      961 aaatttgttc ggaaaagctg gtacatggat tgaacgactg ccttttcatt atcctggtag
     1021 atagcattac caaaataatc aattggataa ggaggagtgt ctgaaaaagt aacgggatag
     1081 cttgttaatg cattaaggtt tgcatttagt acccatatat ttttttctcc caaaacaaaa
     1141 aggagataat taccgttttg gtcttttaaa atcgtattga caccgaaaga agttgttcgg
     1201 cccgggagct tcgcaatatg ccggatcatt tgttgtgtgg tatcaaatat gaataaaccc
     1261 gcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaggcc
     1321 gattacccga ttttcttctt tattgagtga gccaaaatat ttaattgggt aattattgcg
     1381 gattattttt ctgatacctc cgtttattgt ctgcagataa aggttaccaa gataatcttc
     1441 tacaatgttc gcaacagtta gattaccggc aaccggctgg ttctgaaaat tgacgaactc
     1501 tgatttcagc acccggaaac tggaatctgt ttcgtacata tgattattaa ttgaaacaat
     1561 gtatttgttc tgccagcgaa gggtgttgaa ccgggaaaaa tccttgtcta aaaattttcc
     1621 tgtaatcggc agctgtttat tagtgccaga gacaaaatcc cagcattgta aagctgaatt
     1681 ttgggaatgc gtagtataga caaccttatc tccggtaaga agacgaagga agtattggga
     1741 tcttttgtga atagggggcg actggtaaag caattttttg ttttgcaggt cccataaaca
     1801 aatggtttct ttcatccaga atgcgaactt gtgatcaata atattttcgc ttatgcctgg
     1861 aatattttca ctttgtgtac tgtgggaggg catacccgta ttcacggaac tgactatttt
     1921 gaaatttccc cgttgtatac ctataattgt agccttctca tcatagcaat aaatgatccc
     1981 atcatcttca ccaaggaaat aaaccggttt ttgcaggcct gcgggctgct tatatacttg
     2041 aataaaactg tttttatcta tctcgtacct ggatataccc tgtgaatgac tgatcagcag
     2101 gtcaccattg ctgcaacgga atagttttgc atatttatcg tcgggtagcc ccggttgaat
     2161 ttttatcgtc ctgaaattat taccatcaaa ctgttgaata ccattcggga aggttaccca
     2221 gcaaaaatta ttcttgtcca cttcaatatc tccgatagta ctttggagca atccctcatt
     2281 tacattataa ctgaatgtgt tgtacctgtt tagcaactgg gcccggctgc tgnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn gtaagaagat taaaaaattg ccgggctttc tcaagctatt
     2401 gagtttgtaa tatcaaatta aggaatactg ccggaattcc tttgtaatcc aaatggatta
     2461 taggtcaaaa tgagtccctt agatattttc ggatcactca agtatcgcta actgttatgg
     2521 gaatgaacgg aaagccatta tacaagttcc gccagataag ctttattaca ataaagaaga
     2581 ctatttactt ccatattgtt tttcttgccg tatttataat atcgattcaa cctgtaaatg
     2641 cgcaggcgcc ccaggtatta aaagatatca atccttatct gacactttct tcacnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnngagcttt ggaagacaga tggtacagaa gcaaatacgg tcctggtaaa agatattaat
     2821 agcggtgttt catcatccaa cccacagtcc tatacagaaa tgggaggtgt tttatttttt
     2881 atagccacta catcttccaa tggtacagaa ttatggaaga cagatggtac ggaagcaaat
     2941 actgtattag taaaagatat taatacagga acagcaagtt cttcacctgc caacctaaag
     3001 gttataaatg gagtgcttta ctttatcgcc acttctgcca ccagcggccg ggagctttgg
     3061 cgaagcgatg gcacagaggc aggtacgttt ctggtcaann nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttttatt ttacggctga
     3181 cgataatatt aacggagagg agttatgggt gtctgacggc accactgcag gaactttttt
     3241 attaaaagac atttatcccg gattttctcc ttcatatatc tcttacctga cgccggttgg
     3301 aaatactgtt tttttccagg cagaggatga tgtaaacgga ttagagcttt ggaaatcaga
     3361 cggtacacct gcgggcactg tacttgtaaa ggatattgaa ccgggtcttg atggttccaa
     3421 tccttactac ctgataaact ttaacgggac cctctttttc accgctacta ctattgccaa
     3481 cgg
//
LOCUS       DAORLS010000315         3462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14823_length_3462_cov_2.091283, whole genome shotgun sequence.
ACCESSION   DAORLS010000315 DAORLS010000000
VERSION     DAORLS010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14823_length_3462_cov_2.091283"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK977_17250"
     CDS             <1..396
                     /locus_tag="PK977_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19928.1"
                     /translation="TICEVTKAEAEQQYPNYWFYKARETKYCWKATKTGDPNYPQYLW
                     GVPQSMKSNLESTYGYTLSNVDCSSFCNLRWIERHQSKITGLYSANTLWAETLLSSDS
                     CSRLTIGRVIVYRETSDSLITRELTDKKP"
     gene            407..913
                     /locus_tag="PK977_17255"
     CDS             407..913
                     /locus_tag="PK977_17255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF19929.1"
                     /translation="MKSKIILAAISFFAVVKAFAQDQPILPLGTCGIVNVYDAAGNRV
                     KRVYFCNNGVDPYPQRNQQAGGANYPVITGKPNESPSGDKNDTKEFEYVDALYPNPTT
                     GKFYVTFSKYLTNANVSITDNTGKMLAKFTANGYKVDFDLSPYAAGVYYVRIEREGKV
                     ITKKVMKQ"
     gene            1127..>2158
                     /locus_tag="PK977_17260"
     CDS             1127..>2158
                     /locus_tag="PK977_17260"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRF19930.1"
                     /translation="MSLPGEKGLNKKQAPILSLPMQDGKMANFRVWEYEMMEPALAAR
                     FPEIKTFIGYGVDDPFATIRFDYNPYFGFSAQVLSTHGDVYIDPYAKGDTEHYISYLT
                     KDNHRQSSFNCNTVLDSADAHNRPERIQGGICRGSQLFTYRLAIACSGEYATTVCSPT
                     APSVPSTMAAITTTLNRVNGIYEKELSIRFLLIANNSQVVYLNPATDPFTNGNELAMM
                     NECQTTLNSVIGTTNYDAGMVLGTGNSSAASLYEICNSPSKGKAVSGLPNPTGDIFDV
                     GRVAHNLGHLFGAADTHNSSDGNCTGDANTGYEPGSGTTIMAAAPGCGTDNLQPLPDP
                     YFHSTSFDLI"
     assembly_gap    2159..2182
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2274..>3462
                     /locus_tag="PK977_17265"
     CDS             2274..>3462
                     /locus_tag="PK977_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19931.1"
                     /translation="MNTPFQIRGVTATDDDGDPVTFSWEEVDRGPSGIWNSGLTTSAP
                     AFRSRVPQTNNERVFPDMQLILAGFPANPPAIQGGLKGETLSNISRGYTFSVTLRDCR
                     GGVLNAGSNCSTTTFGNYAVFAVPETGPFKVNLPNGGENWSSGSQQTILWDVAGTTGP
                     MINTQFVSIFLSDDGGLTYPYLLASNVPNDGFEVVTMPAITTTTARVKIEGALNIFFD
                     ISNSNFSISPSPVGFEFNTPAMQTVACPGPGTVQFSLGAISNGGYNTPVALSASGVPA
                     GAGISFGTNPVTPGNSSTITLNNVNTVANGTYVITITGLSGTMVRSRVLVLQVQNGTG
                     PVITTQPVSQTDCEGSNVTFTVAATSAVSYQWQISADGGFNFSDITTGGNSSALIVTN
                     IGAQ"
BASE COUNT         1047 a          683 c          768 g          940 t
ORIGIN      
        1 accatttgtg aagtaacaaa agcagaagct gagcagcagt accccaatta ctggttttat
       61 aaagctaggg aaacaaaata ttgctggaaa gccaccaaaa ccggagaccc caactatcca
      121 caatatttat ggggtgtacc acagtcaatg aaatcaaacc ttgaaagtac ttatggctat
      181 acgctttcta atgttgactg cagcagcttt tgcaacctaa gatggataga gagacaccaa
      241 agcaaaataa ccgggttata tagcgctaac acactatggg cagaaacatt gctttcatcg
      301 gactcttgtt caaggcttac tattgggcgg gtgatcgttt atagggaaac atccgattct
      361 ttaataacaa gggagctaac agataaaaaa ccttaaaata ttttgtatga aaagtaaaat
      421 tattctggcc gctattagtt tttttgccgt tgttaaggct tttgcacaag accagcctat
      481 tcttccactt ggcacatgtg gtattgtaaa cgtttatgat gctgccggca accgtgttaa
      541 gagagtttat ttctgtaata acggcgttga cccttatccg caaaggaacc agcaggctgg
      601 cggcgcaaac taccctgtga taacagggaa acccaacgag tccccttcag gagacaagaa
      661 cgacaccaaa gaattcgagt atgtagatgc tctatatccc aatcccacaa ctggaaaatt
      721 ttatgtaact tttagcaagt atcttactaa tgcgaatgtc agtattacag ataatactgg
      781 caagatgctg gctaagttta cagcaaatgg gtataaggtg gattttgatt tatcgcctta
      841 tgcagcgggt gtgtactatg taagaattga gagggagggg aaggtgataa cgaagaaggt
      901 gatgaagcag tagtcaatag taaatgaaag ataaatgagt aaaggtaaat tttggttaat
      961 aggggttttg gttttttgtt gtaattgttc aaatgtatat ggacaaggct cttttttttc
     1021 tgcggcggat gcagagaaaa ccaaattatc aaatacagaa aggaagatta tacccggaaa
     1081 gtattttaca tcttctttaa acgttgcttc gctcaagaac tttttaatgt ctttgccagg
     1141 cgagaaggga ctgaacaaga aacaggcacc catactttca ttgccaatgc aggatggtaa
     1201 aatggcgaac ttcagagtat gggaatatga aatgatggaa ccggcgctgg cggcaaggtt
     1261 tccggagatt aaaactttta ttgggtacgg tgtagatgac ccttttgcaa cgatacgttt
     1321 tgattataac ccatactttg gcttcagcgc acaggtatta tctactcatg gtgatgttta
     1381 tattgaccct tatgcaaaag gagatacgga gcattacata agctatttaa caaaagacaa
     1441 tcaccgacaa agttccttta attgcaatac tgtcttggat tcagcagatg cacataacag
     1501 acctgaaaga attcagggag gaatatgtcg gggcagccag ttatttactt accgcctggc
     1561 catagcctgt tcgggggagt atgctacgac agtttgctca cctactgcac cttctgtccc
     1621 gtcaacaatg gcagcaataa ccaccaccct taacagagtt aatggtattt atgaaaagga
     1681 gttgtctatt cgttttcttt taatagccaa caatagccaa gtagtttacc ttaacccagc
     1741 aactgatcct tttacaaacg gaaatgaatt ggcaatgatg aatgaatgcc aaacaacatt
     1801 aaactcggta atcggcacaa caaactatga tgccggtatg gtattaggta ccggtaatag
     1861 cagtgcagct tctttgtatg aaatttgtaa ttcacccagc aaagggaaag cagtaagcgg
     1921 tttgcccaat ccaacaggag atatttttga tgtgggaaga gtggcgcata atcttggcca
     1981 tctttttggg gcagccgata ctcataacag cagtgatgga aattgtacgg gcgacgctaa
     2041 tacagggtat gagccaggaa gcgggacaac cataatggca gcagcacccg gctgtggtac
     2101 tgacaacctt cagccacttc ctgatcctta ttttcattca acttcttttg atttaattnn
     2161 nnnnnnnnnn nnnnnnnnnn nntggtacag gggcaggttg tcatgggatc attaatacgg
     2221 gtaatacccc tccacggttt acaaataatg ttattttagg ggcaaacatt ccaatgaaca
     2281 ctccatttca gattcgggga gttactgcca cagatgatga tggcgaccct gtaacattca
     2341 gttgggaaga agtggacaga ggaccttcgg gaatatggaa cagtgggtta actacttcgg
     2401 cacctgcatt caggtcaagg gtcccgcaaa caaacaatga aagggtattc cctgatatgc
     2461 aattaatcct tgccggattc ccagcaaacc caccggctat acaagggggg ttaaaagggg
     2521 aaactctttc caatatcagc aggggatata ctttttctgt aactctgcgt gattgcagag
     2581 gcggtgtatt aaatgcaggc agtaattgct ctactactac ttttggcaat tatgctgttt
     2641 ttgcagttcc cgaaaccggg ccttttaaag tgaacctacc aaacggggga gaaaattggt
     2701 cgtcgggttc acagcaaaca atactttggg atgtggcagg caccacaggg cctatgatca
     2761 atacacaatt cgtcagtata tttttatcag atgacggagg tttaacttat ccctatcttt
     2821 tggcatctaa tgtacctaat gacggttttg aagtagtaac tatgcccgcc attacaacta
     2881 ctacagcaag agtaaaaata gaaggggcat taaatatctt ctttgatatt tcaaacagca
     2941 acttctccat ttcaccttca ccggtgggtt ttgaattcaa cacaccggct atgcagactg
     3001 tggcatgtcc cggcccaggt acagttcagt tttcattagg cgctatatca aatggcggtt
     3061 acaacacacc tgttgcactt agcgcttccg gtgtaccggc aggggcaggt atttcatttg
     3121 gtacaaatcc cgttacaccc ggaaactcca gtacaattac attgaacaat gtaaatactg
     3181 tagctaatgg tacttatgtt attactatta caggattatc cgggacaatg gtacgatcaa
     3241 gagtactggt actacaggtg caaaacggga cagggccggt tattaccaca caacctgtct
     3301 cacaaacaga ttgtgaggga agcaatgtga catttacagt agcagcaact tctgccgtat
     3361 cttatcaatg gcaaataagt gctgatggcg gatttaattt cagtgatatt acaacaggtg
     3421 gaaattcatc tgcgctgatt gtaactaata tcggagcgca ac
//
LOCUS       DAORLS010000316         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_14988_length_3431_cov_1.653140, whole genome shotgun sequence.
ACCESSION   DAORLS010000316 DAORLS010000000
VERSION     DAORLS010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14988_length_3431_cov_1.653140"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK977_17270"
     CDS             <1..189
                     /locus_tag="PK977_17270"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRF19932.1"
                     /translation="GKSGYGRPGEKKNVDIVYTVITRLELNKLNSEINRIEPDAFVVM
                     SSVKDTKGGMIKKRRLKH"
     gene            complement(203..>1037)
                     /locus_tag="PK977_17275"
     CDS             complement(203..>1037)
                     /locus_tag="PK977_17275"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF19933.1"
                     /translation="YFQKLLIHSLLYVLFVVLCILIVGLIGALFNKNDGSYWVKFKKD
                     VFSLRVYRLLIAWYFIVIFTFFILDVSERYGAATLKRLLLGKYHAPGKEHRIFMFLDL
                     KSSTAMAERIGDELYFRMLRYFYQVANEAIMQANGEIYQYVGDEIVISWEKDTGLKNA
                     NCIRCFDIIREAVQKRRAYFLDNFTVVPDFKAGVHSGIVATGEIGLVKKDIVYSGDVL
                     NTTARIIALCNHYRQDMIISETLYQQLKDTPGYRFTYLDSPLLKGKAAPFAIFGVKAL
                     S"
     assembly_gap    1038..1047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1048..1287)
                     /locus_tag="PK977_17280"
     CDS             complement(<1048..1287)
                     /locus_tag="PK977_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19934.1"
                     /translation="MIQGPVLWYRLRQVMFITIAWVIIGVLVELYNSVNYDPEGNRHF
                     VWFLFGNGAAEHLLITAIGPLMGGLVGGSFIVFFQR"
     gene            1379..>1903
                     /locus_tag="PK977_17285"
     CDS             1379..>1903
                     /locus_tag="PK977_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19935.1"
                     /translation="MHKVISGLALLFCVSVVQAQDVDYKKGIIQVNGKDYAKLEVTKQ
                     NFGLTKSFEVFSLAGEKIIIAAPATEFENDKSDNSYLFYRLTFLTADQVGIFKVASLG
                     QEKSFAKLIGTSGILINDKADAIKVKEFIAAKGASPKIAVNYALVARSTSWPITIKAD
                     KTIEQNAKIIGNFKP"
     assembly_gap    1904..1919
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1920..2163
                     /locus_tag="PK977_17290"
     CDS             <1920..2163
                     /locus_tag="PK977_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19936.1"
                     /translation="SSNGLDMYEFALPSGVVVAKVSFTGGNNAQNMELFTAKDNLKRT
                     VPIPQQDKILAADTSIDKNYFTLKRIVKWLVDNQYL"
     gene            2250..>2755
                     /locus_tag="PK977_17295"
     CDS             2250..>2755
                     /locus_tag="PK977_17295"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRF19937.1"
                     /translation="MIRKKLFRYAKKPVLFRERGKEILRIEALSDAVFAFSVSLLVVA
                     LEVPQNFEELKTILQGAVPFFATVAMIFLFWYQQYKFFRRYGLNDVGTILLNLGYLAV
                     ILFYVYPMKFLFSLLLGSWTGIDLFPKAAEKGIAVIHAEDFSQLIILFSTGYAIIWFL
                     LYGMYQRAG"
     assembly_gap    2756..2766
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2767..2998
                     /locus_tag="PK977_17300"
     CDS             <2767..2998
                     /locus_tag="PK977_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19938.1"
                     /translation="QLELSAYELKFTRKEKRGAFWNGLIGVAAITMAIAGMESIAGIC
                     YLFIPVLLVLNEKLFKKSLHIGKKEASGSHNV"
     assembly_gap    3065..3094
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3150..>3431
                     /locus_tag="PK977_17305"
     CDS             3150..>3431
                     /locus_tag="PK977_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19939.1"
                     /translation="MSTKDKKIIEDSKAAKEEFIKADGLMKNLFDNAYAYVILPNVGK
                     GGIGIGGAAGNGAVFQKGSLIGMAKMTQVTIGFQWGGQAYREVIFFETKA"
BASE COUNT         1081 a          674 c          669 g          940 t
ORIGIN      
        1 ggcaaatcag gatatgggag acctggtgag aaaaagaatg tggatattgt ttacacagtt
       61 atcacccgtc ttgaactaaa taaactcaac agcgagataa acaggattga acccgatgct
      121 tttgtagtaa tgagcagcgt aaaagatacc aaagggggca tgatcaaaaa aagaaggctg
      181 aaacattaaa cgaatgctct tgtcatgaaa gcgccttgac cccaaatatt gcaaacgggg
      241 cagctttacc ttttaacaaa ggactatcca ggtaagtaaa ccggtagcca ggtgtatctt
      301 ttagttgctg gtaaagtgtt tcagatatta tcatatcctg ccggtaatga ttacataatg
      361 caatgatcct ggctgtggta ttgagcacat cgccagaata cacaatatcc tttttaacta
      421 ggccaatttc accggttgca acaataccac tatggacacc tgctttgaaa tcaggaacaa
      481 ctgtaaaatt gtcgagaaag taggcccttc ttttttgaac agcttccctg ataatatcaa
      541 aacaccgtat acagtttgca ttctttagac ctgtatcttt ttcccaggaa ataacaatct
      601 catcacctac atattgataa atctccccat ttgcctgcat aatcgcttcg ttcgctacct
      661 ggtaaaaata acggagcatc ctaaagtaca actcatcgcc tattctttcg gccatagcag
      721 tggaagattt aaggtcaaga aacataaata tccgatgctc tttaccggga gcatgatact
      781 tcccaagcaa aagccttttc aaagtagccg ccccgtatct ttcgcttacg tcgagaataa
      841 aaaaggtgaa aatgacaata aaataccatg caatcagtaa tcggtaaacc cggaggctga
      901 aaacatcttt tttgaatttt acccaatagc ttccgtcatt cttgttaaac aatgcgccga
      961 taaggccaac aattaaaata caaagaacaa caaagagaac atagaggaga gaatggatca
     1021 gtagtttttg aaaatatnnn nnnnnnnccg ctgaaagaat acgataaaag aacctcccac
     1081 caaaccaccc atcagcgggc caatggctgt gatcagtaaa tgttcagctg ccccgttgcc
     1141 aaataaaaac cagacaaaat gcctgttgcc ttccgggtca tagtttacag aattatacag
     1201 ctcgactaaa acgccgatga tcacccatgc gatagtaata aacattacct gccgtagcct
     1261 gtaccataag actggtcctt gtatcatttt ccaatattaa tgaagagtat tcaagcaccg
     1321 aagaaaaaaa ctaaaaacaa cgacactgtt tattgcttat ctttccccaa aaaacaacat
     1381 gcataaagtt atttctggat tagcgctgtt attttgcgtt tcagttgtgc aggcacagga
     1441 tgttgattat aaaaagggaa ttatacaggt aaacggtaag gattatgcca aactggaggt
     1501 aacaaagcaa aactttggcc tgacgaagtc cttcgaagta ttttctcttg ccggtgaaaa
     1561 gatcattatt gccgcaccag ccacagagtt tgaaaacgac aaaagcgata actcttattt
     1621 attttaccgc cttacttttc ttactgccga ccaggttggg attttcaaag tagcctctct
     1681 cggacaggaa aaatcttttg ccaaattgat cggaaccagc ggtatcttaa taaatgataa
     1741 ggcggacgcc attaaggtaa aagaattcat cgctgccaaa ggtgcatcac caaaaattgc
     1801 tgtgaattat gctttagttg caagaagtac cagctggcca atcactataa aagcagataa
     1861 aaccatcgag caaaacgcaa agatcatcgg caattttaag ccannnnnnn nnnnnnnnnc
     1921 agcagcaatg gcctggatat gtacgaattt gcccttcctt ccggcgttgt tgtagcaaaa
     1981 gtttccttta ccggcggcaa taatgcacaa aacatggaat tatttactgc caaagacaac
     2041 ctgaagcgta cagtaccgat tcctcagcag gataaaatac tggctgctga caccagcatc
     2101 gacaaaaact attttaccct gaaaaggatt gtaaaatggc tggttgacaa ccagtatttg
     2161 taatatttat aagttcatta gtatagctaa cataaatcat atactgttcc cgtcggtact
     2221 ggtaatttgc ctctcttaat aaagtagcta tgatccggaa aaagctgttc cggtatgcca
     2281 aaaaaccggt tcttttccgg gaaagaggaa aagaaattct gcggattgaa gcactgagcg
     2341 atgctgtttt tgccttttca gtttcgttat tagtagtggc gcttgaagta ccacaaaact
     2401 tcgaagaact gaaaaccatc ttgcagggag ctgttccttt ttttgctacg gtagccatga
     2461 tctttttatt ctggtaccag caatacaaat tctttcgccg ttatggtctg aatgatgttg
     2521 gcaccattct gttgaacctg ggttatctcg ccgtaatatt attttatgtt taccccatga
     2581 aattcttatt ctcattattg ctgggttcat ggacaggtat cgacttattt ccaaaagctg
     2641 ccgaaaaagg cattgccgtt attcatgcag aagatttttc tcagctcatt attttattca
     2701 gtaccggtta tgcaataatc tggtttctgt tatatggaat gtatcaacgg gctggnnnnn
     2761 nnnnnnccaa cttgaactaa gcgcctatga gctaaaattt acccggaaag aaaaaagagg
     2821 tgccttttgg aatggactta taggtgtggc agccataacg atggccattg cagggatgga
     2881 aagcatcgcc gggatatgct atttattcat tccggtatta ctggtactga acgagaagct
     2941 gtttaaaaag tctttacata ttggtaaaaa agaagcttcc gggtcacata atgtgtaaga
     3001 agatttaatt tggcctcatc tttgacacag cccttttgta aatcaaaata gttagttatg
     3061 acgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnttacgc ttttttagcc atcacatttt
     3121 ttgcatcaac tataacgata acagcccaat tgagtaccaa ggacaaaaaa attattgaag
     3181 attcaaaggc agcaaaagaa gaattcataa aagcggatgg cctgatgaag aacttatttg
     3241 acaatgccta tgcttatgtg attttaccca atgtaggtaa aggtggtatt ggcataggcg
     3301 gtgccgccgg taatggtgct gtttttcaaa agggctctct tattggaatg gctaaaatga
     3361 cacaggtgac cataggtttt caatggggtg gacaagcata tcgggaagta atattttttg
     3421 aaacaaaggc c
//
LOCUS       DAORLS010000317         3415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15098_length_3415_cov_2.191964, whole genome shotgun sequence.
ACCESSION   DAORLS010000317 DAORLS010000000
VERSION     DAORLS010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15098_length_3415_cov_2.191964"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK977_17310"
     CDS             <1..369
                     /locus_tag="PK977_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF19940.1"
                     /translation="EKAHAGLPLVQHPLLLMLNYYKEGRISLDKIVEKMSHAVAECFQ
                     VKERGYIREGYHADLVMVDLNNSSTVSKDNILYKCGWSPLENFTFPASITHTFINGHL
                     VYDNGKWNESQMGQRLVFER"
     gene            432..1748
                     /locus_tag="PK977_17315"
     CDS             432..1748
                     /locus_tag="PK977_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF19941.1"
                     /translation="MQIDHQSFNDISIFHPEEEFSIFHKLNFTRTVGGREWLRKFFSE
                     PHNDLKKIIGTQKVIRTFMEHIQHWPSEITNGTVLVIDKFLDYPLDPISEAPASLNNI
                     FYKWLHNADYSMIKYSVPHFADFFRGIRKVAQLLEDLDLPANIKLYIDRINGLLKEPP
                     LQKLSETEAGQRFSIRQNLYFAYHLRGRYRTNTLEMIDTFSRLDAWYSMAVAVKTYQL
                     SFPEFVEQDTPLVDAKGLYHILLPQPVAYDLHMSPEQNFLFLTGANMAGKSTLIKSVG
                     AAVFLAHIGMGVPAEKMKLTLFDGLLSNINVVDNIAKGESFFFNEVQRIKNTIEKIND
                     GKKWLVLIDELFKGTNVQDAMKCSLTVIKGLIKIKNSLFILSTHLYEIGEELKTYPNI
                     SFRYFETTVHDDQLSFSYQLKEGISNDRIGYVILKREKVVDMLEKL"
     gene            1865..>3415
                     /locus_tag="PK977_17320"
     CDS             1865..>3415
                     /locus_tag="PK977_17320"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HRF19942.1"
                     /translation="MSSYYRLRPSGFALIIAILFSFQQISAQCSNSNPAGCSCPGGGT
                     SCLLLPDITAGKKTLNATTGWTEYTQAAAAPNKGLLRLDVSTPNIGWGPVETIPTNNY
                     LCGTDTLYNFVPPSGFLCPDGSFPKRLIKQRLYQKNGNSFQFIDRDAGWMVYHPSHGH
                     IHIEGWGLYTLRLRDVSVVDTLQWPVVNSGVKVSFCLIDLTTCSGSAGDCQDANGNTL
                     LNNNFPNYGLGGGYGCNNVKQGISVGKVDIYHQYLDESFVKIPYEACNGAYHAVVQVD
                     PDNHFLEMNDNNNWLAAPVNLTKQRTSSTNPYAYIFSKKGNTVCQGSTLELEASGASN
                     YVWSTGATTQKITISQPGKYWVRATTPCGVATSDTLDIAQSGISAFPAITTNDTICAG
                     ERAHLYASGNAHWYDAPTGGNLVFIGNNYETGSLFNNTTFYVADQPSVLGGNMAPSST
                     TFSGTGNYSTARTEYLIFNAFLPFKLKKIKVDASAAGTRIFQLRDQYGHLLQQKSLTL
                     AAGVQDVTL"
BASE COUNT          995 a          739 c          767 g          914 t
ORIGIN      
        1 gaaaaggcgc atgccggatt gccgctggtg cagcatcccc tgctgttaat gttgaattat
       61 tacaaagaag gaaggatttc gctggataaa atagtggaga agatgagtca tgcagtagct
      121 gaatgtttcc aggtaaagga aagaggttat atccgggaag gctatcatgc cgacctggtg
      181 atggtggacc tgaacaacag ctcaacagtt agcaaagaca atattttata taaatgcggc
      241 tggagtcctt tagagaattt tacgtttcct gcatctatta cacatacgtt tataaacggc
      301 catctggtgt atgataacgg caagtggaac gaatcgcaaa tggggcaacg gttagtattt
      361 gagaggtaag attttagatt gaagattggc tcctaaatat tcaatcttcc atattcaatt
      421 ttcaatcggc tatgcagata gaccatcaat ccttcaacga catttctatc ttccacccgg
      481 aagaagagtt ctccattttt cataaactga attttacacg aaccgttggc ggcagggaat
      541 ggctgcgaaa atttttcagt gaaccgcata atgacctgaa gaagatcatc ggcacacaga
      601 aggtgatccg cacttttatg gaacatatcc agcactggcc ttcagagatc accaatggca
      661 cggtactggt cattgataaa tttctcgatt acccgcttga tcctatcagc gaagcgccgg
      721 cctctttgaa caacatcttt tataaatggc tgcacaatgc ggattattcg atgatcaagt
      781 attccgtacc gcattttgca gattttttcc gtggcatccg caaagtagcc caactgctgg
      841 aagatcttga ccttcctgcc aacatcaaat tgtatattga ccgtatcaat ggtttgctga
      901 aagaaccacc gctgcaaaag ctctccgaaa cagaagccgg tcagcgtttc tccatccgac
      961 agaacctcta tttcgcttat cacctgcgtg gcagataccg taccaacact ctggagatga
     1021 ttgatacctt cagccggctg gatgcctggt attcgatggc cgtcgctgta aaaacgtacc
     1081 aactgagttt tccggaattt gttgaacagg atacacccct ggtagatgca aaaggtttat
     1141 accatatcct gcttccccaa ccggtagcct atgatctgca catgagccct gagcagaatt
     1201 ttcttttttt gaccggcgct aatatggcag gcaagagtac actgattaaa tcagtgggtg
     1261 cggctgtatt cctggcgcat atcggcatgg gtgttccagc tgaaaagatg aaattgactt
     1321 tgtttgacgg gttactgagc aatatcaatg tagtagacaa tattgccaaa ggcgagagct
     1381 ttttctttaa cgaagtacag cgtatcaaga atacgattga aaagatcaat gacgggaaaa
     1441 aatggctggt actgattgat gaattattta aaggcaccaa tgtacaggat gccatgaaat
     1501 gttctcttac agtcatcaag gggttgatta aaatcaaaaa ctctcttttt attctttcta
     1561 cgcatttata tgaaattggg gaagagctga agacgtatcc caatatttca ttccgttatt
     1621 ttgaaactac ggttcatgat gatcagttgt cattcagtta ccagctgaaa gaaggtatca
     1681 gcaacgaccg gattggttat gtcattctta aaagagaaaa agtggtggat atgctggaga
     1741 aactctgaag taccagcaag ctaaaagtat acatggagga aactggttgt agtagaaata
     1801 ctactcatcc gtattgatta taatttatac atttaaggac actttcacca ccaacaactg
     1861 actcatgagt agttattaca ggttaaggcc atccggcttt gcattgatca ttgccattct
     1921 tttttcattt cagcaaatat cagcccagtg cagcaatagt aacccggcag gctgctcctg
     1981 ccccggaggt ggcaccagtt gcctgctgct gccagatatc actgccggca aaaaaacact
     2041 gaatgccacg acaggatgga cagaatatac acaggcagct gctgccccca ataaagggtt
     2101 gctgcgcctg gatgtttcga ctccgaatat tggctggggt ccggtagaaa cgattccaac
     2161 caataactat ttatgcggaa cagatacact ctacaatttc gttccgccat ctggttttct
     2221 ttgccctgat ggttcttttc ccaaaagact gatcaaacaa aggttatatc aaaagaacgg
     2281 caacagtttc cagttcatag acagggatgc agggtggatg gtatatcacc catcacacgg
     2341 acatattcat attgaaggct ggggattata cacacttcgt cttcgtgatg taagcgtcgt
     2401 tgatacactc caatggccgg tggtaaacag cggggtaaaa gtgagctttt gcctgatcga
     2461 tctgactacc tgcagtggca gcgccggcga ttgccaggat gccaacggaa acaccttact
     2521 gaataataat tttcccaact acgggttagg tggtggttat ggttgtaata atgtgaagca
     2581 aggtatcagc gttggtaaag tagatatcta tcaccagtat cttgatgaga gttttgtaaa
     2641 gatcccctat gaagcctgta acggtgctta tcatgcggta gtgcaggtgg atccggataa
     2701 tcatttcctg gaaatgaatg ataacaacaa ctggctggct gcgcctgtta acctgaccaa
     2761 acaacgaacc agcagtacaa atccctatgc ctatattttc agtaaaaaag gaaataccgt
     2821 ttgccaggga agtacactgg aactggaagc cagcggcgca agtaattatg tatggagcac
     2881 cggtgctact acacaaaaga tcaccatcag tcagcccgga aaatattggg taagggccac
     2941 tactccctgt ggagtggcta ccagtgatac gctggatatt gcccagtcag gtatcagtgc
     3001 atttccagcc attacaacca atgatacgat ctgtgccggt gaaagagctc atttatatgc
     3061 cagcggaaat gcacactggt atgatgcacc caccggaggc aatcttgttt ttattggcaa
     3121 caattatgaa accggcagcc ttttcaacaa tacaactttt tatgtagccg atcagccatc
     3181 tgtattgggt ggcaacatgg caccttcctc taccaccttc agtggcacgg gtaattattc
     3241 aaccgccaga accgagtatc tcatatttaa tgccttcctt ccattcaaac tgaagaagat
     3301 aaaagtagat gcatcggcag ctggtacacg gatctttcag ttgagggatc aatacggtca
     3361 tctgcttcag caaaaaagct taacacttgc agcaggtgta caggatgtaa cactg
//
LOCUS       DAORLS010000318         3408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15153_length_3408_cov_1.592007, whole genome shotgun sequence.
ACCESSION   DAORLS010000318 DAORLS010000000
VERSION     DAORLS010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15153_length_3408_cov_1.592007"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK977_17325"
     CDS             complement(<1..355)
                     /locus_tag="PK977_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19943.1"
                     /translation="MKKKNLHAAAWLLLPAVVVSMLLASCDAYNFSRPQPADRENIYV
                     FPDEMLGKWKEDTFTTGIDFSVPLNNNNGDKYIDNTQPLPADEDEGVYIITKHYVSLI
                     FTSGEKIVKGAWPQLT"
     gene            583..1233
                     /locus_tag="PK977_17330"
     CDS             583..1233
                     /locus_tag="PK977_17330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRF19944.1"
                     /translation="MAVFKIGFLLVALVTGRLQGWKGLEGFGLGWRMGWWQQLLKGLL
                     LGFLFFGLAEGLSVAFGYEKFNGIAATEVILKQLPLILLMTFFPSIAEDLLTRGYLYG
                     HLFSKMKPWHWVLLSAVVYVLNHIWRLTENPSVLVYLFILGLVLAFTVWETRALWLAL
                     GIHWGANIAFESTHSILQTESAVSNYGSTWILAVVWLLLFLVLLLGRTGSPALRKV"
     gene            complement(1423..>1723)
                     /locus_tag="PK977_17335"
     CDS             complement(1423..>1723)
                     /locus_tag="PK977_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF19945.1"
                     /translation="APFNNAANINAISDPAQISALANILKYHVTDSRYFTWDLGILSQ
                     ITTIAPGPNNKIKGILGLNKGWVKGNINSNFSQITPSNILAVNGVVQVIDRVLLP"
     assembly_gap    1724..1780
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1781..2551)
                     /locus_tag="PK977_17340"
     CDS             complement(<1781..2551)
                     /locus_tag="PK977_17340"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF19946.1"
                     /translation="MKKLITRSALLVAACAILFASCKKEVSTGIAVTQNEEEKLKAEA
                     QNAVSARQSDAADFEKEFALYRDNITESGQQNIVQIAQSLPIFRSLVAAVVKTGLAGT
                     LSSASLNATVFAPTDAAFAKLPAPFNNQTNINNITDPAQIDALRNILLYHVLGSEVRR
                     NQVPGGRSSAATLKPEVISNDNKIYFSNTLGLLLVNGNSLVLLANVDASNGIIHVIDD
                     VLLPPTQTIAGIATANPAFSTLVAALVKTNLVGAFTGTG"
     assembly_gap    3002..3174
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3184..>3408
                     /locus_tag="PK977_17345"
     CDS             3184..>3408
                     /locus_tag="PK977_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19947.1"
                     /translation="MDIWVECSAENYKKLVKAFQQFHMPVFDMTLEKFLQVNESDVFS
                     FGRNPVGIDIMTAVKGLNFDEAYKLSLIFND"
BASE COUNT          834 a          619 c          715 g         1010 t
ORIGIN      
        1 tggttaactg cggccatgcc cctttcacaa tcttttcacc tgaagtaaaa atgagcgata
       61 cataatgctt tgtaatgatg tagacgcctt catcttcatc agcaggtagc ggttgggtat
      121 tatcaatgta tttatcaccg ttattattat taagcggaac agaaaaatca ataccggtgg
      181 tgaaagtatc ctctttccat tttcccagca tttcatccgg gaatacataa atattctccc
      241 ggtctgccgg ctgcggtcgg gagaaattat aggcatcgca ggaggccagc agcatactca
      301 caacaactgc tggtaataac aaccaagcgg ctgcatgcaa atttttcttt ttcatcggaa
      361 ggctttttgt caaggtacaa cgctgtaaca gccttttgta ctgacagccg tcatgtttgg
      421 cactaagtcc tgtttttcat taaataaaaa actaaggtct tagctatctt taagccttca
      481 atcagtttcg gtatgcgtcc aaaaattgtt tccttccttg caggttttct gctgttgttt
      541 ttcatttacc actttccaga attttttacc gcattctgga tcatggcagt ttttaaaatt
      601 gggtttctgc tggtagcatt ggttacaggt cgtctgcaag gctggaaagg actggaaggt
      661 tttggactgg ggtggcgcat gggctggtgg cagcagttgc taaaggggtt gttgctcggg
      721 ttcttgttct ttggtttggc agagggtttg tcggttgctt tcggttacga aaaattcaac
      781 ggaatagctg ccacagaagt gatattaaaa cagctgccgc tgatcttgct aatgactttt
      841 ttcccttcca ttgcagaaga tttattgaca aggggttacc tgtacgggca cctattttca
      901 aaaatgaagc cctggcattg ggtactttta tctgctgtgg tatatgtgtt gaatcatatc
      961 tggcgactta cggaaaatcc ttctgtgctg gtatatcttt ttatactcgg cttagtattg
     1021 gctttcaccg tctgggaaac cagagcactt tggctggccc tcggcattca ttggggcgcc
     1081 aacattgcgt ttgaaagcac tcattccata ttgcaaacag aatctgcagt gtctaactac
     1141 ggaagtacat ggatactggc agttgtatgg ttgttattat tcctggtact gttactgggc
     1201 cgaacaggct ctccggccct tcggaaagta tagcttctcg caatgtttga gttttgctat
     1261 tgtattaaaa gtgcagcagg gcagtttatc tattattaat tgtagttaat ttttttgcat
     1321 ggcttcgttc aaagaatacc gcatggataa aatagttttc cgaataaaaa aacaacctgc
     1381 tttttcagca ggctgttttt atcttcaaaa ccaaaccaac cactagggaa gcagtacacg
     1441 gtcaataacc tgcactacgc cattcactgc aagaatattt gatggggtaa tttgagaaaa
     1501 attagaattt atatttcctt ttacccagcc tttattcagg ccaagtatgc cttttatttt
     1561 attattgggt ccgggagcaa tggtagttat ctggctcagt attccaaggt cccaggtaaa
     1621 gtagcggctg tcagtaacat gatatttcag gatattggcc aatgcactga tctgggccgg
     1681 gtcgctgata gcattgatat ttgctgcatt attaaaaggc gcannnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccagtacct gtgaatgcgc
     1801 cgacaagatt tgttttaaca agggcggcta caagcgttga gaatgccgga tttgctgtag
     1861 cgatgcctgc aattgtttgc gtaggaggca acagtacatc gtcaattaca tgaatgatac
     1921 cgtttgaggc atctacattg gcaagcagta caagtgagtt gccgtttacc agcaataagc
     1981 ccagggtgtt tgaaaaataa attttgttgt cgttgctgat tacttctggt tttaaagtag
     2041 cagccgaact tcttccacca ggcacctggt tgcgtcttac ttcactgcca agaacatgat
     2101 aaagcaggat atttctgagt gcatcgatct gtgcaggatc agtgatatta ttaatatttg
     2161 tttgattgtt gaaaggtgcc ggcagtttgg caaatgctgc atcagtgggt gcgaatacag
     2221 ttgcattaag agaagctgat gacagggtac ctgccagccc ggtttttacc acagctgcta
     2281 ccagtgatct aaagatgggc aggctttgcg ctatttgaac aatattctgc tggcctgatt
     2341 ctgtgatatt gtcacggtac aaggcaaact ctttttcaaa atcagcagcg tcagactgcc
     2401 tcgcactaac cgcattttgt gcttcagctt ttagcttttc ttcctcattt tgtgtaacag
     2461 caataccggt gcttacttct tttttacacg aagcaaacaa aatagcacag gcggcaacca
     2521 ataaggcgct cctggtaata agttttttca taacgtaatg tgttttagtt attgtgatag
     2581 ataggttgag ttaattcaac acaaatacta atctgaaaaa ttacccggtg gggtgagttg
     2641 caggaattgc gtagtaaatt gttcaatttc ggggataagt cgtagcggcc tgtaaggcaa
     2701 gcattttata aaggccccag cgcagtgcag gccctggttc tctcatcaac tgtacttaat
     2761 ccaggcatat tcaaagtttt cttatgccct gcggacaaat tccgttaatg cgtaatttgg
     2821 attaatttta ttgcatggct gctcccaata aataccgcat ggataaaacg gctttcaaag
     2881 tgcagacttt tgaggaagca gatgatgcta tgcaggatta ttccagttac tcaatgcagg
     2941 aacggttacg gatttactgg tatctcacca gtattgccta taaattcgat ttggataacc
     3001 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaccggt
     3181 gatatggata tctgggtgga gtgttcagcg gagaattata aaaaactggt caaggctttt
     3241 caacaatttc atatgcccgt atttgatatg accttggaaa aatttctaca ggtcaatgaa
     3301 agtgatgttt tttcatttgg gcgtaatcct gttggtattg atattatgac agctgttaaa
     3361 ggattaaact tcgatgaagc ctataagctg tctttaattt ttaatgat
//
LOCUS       DAORLS010000319         3407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15161_length_3407_cov_1.894093, whole genome shotgun sequence.
ACCESSION   DAORLS010000319 DAORLS010000000
VERSION     DAORLS010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15161_length_3407_cov_1.894093"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3079)
                     /locus_tag="PK977_17350"
     CDS             complement(<1..3079)
                     /locus_tag="PK977_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF19948.1"
                     /translation="MLAFALIPVRSEFFIETDYLPEHFFFSKTADKRARISKDVTDFP
                     VRSITGNAVFYLGKKVANAVPYNDNLTIYLRLAAILLLFIFIYRYTESFSRKNGAWKA
                     IGLLGVLLLATRLISYLYPALLNLRQFEFFSPLIYGSNAIQRSLGDLVINSILFCWLV
                     LFAWSKLKSEENLSKDFSFRSKLIAGLLALVFLIFSTFILASVIRSLVADSKISFDVT
                     NFFSLNQYTVAGFFVLACLSLSYYYFSQLLFRLIFPLLAGRRFMVYFIIGFAGLLYLT
                     TRSGNPEVLFYLPVLIWLLIYAWLINSKGVFLNRIRINIASILTWIFIFSASIAAIML
                     SQNSRVEWEKRKSIAEKLAVQTDPSSEMMMSIAVKYLDNDFLSENFHRFHKEEEGQAL
                     KDSIITGNYSGFLNKYDTRLYVYDSANRALFNEDPTSYEALNVIFTVQSKPTATEGLY
                     YYETSFDKFNYIIRREVKDTSGKKMGSFFIVSNLKNFSRDALFPELFRQSKGTDPENS
                     PIYSNAVYIKNRLVSPPGNYPFATWLNDEEIPKEEYRLKTNGDYDELWYRASKEKVVV
                     IARKRETTIETITLFSYIFCSFLFLVALLQFLSLIVRTGTNWQGFRKLFQINIRSQVH
                     STIIFISVFSFLVIGAATISFFISRNRQSNNDKLSRTMKIMVNEMEKKMSDHNTFDDV
                     VKIYDSVSNNGLQKLVDEVSDIHGVDVNVYDLDGNLQVSSEANVYTKGVLSKKMEPRA
                     YFHLSRLRQVQHVQEEKVGNFSFLSIYSPVRNELGEVEAFVNIPYFTSKPELRQEISN
                     FLVTIINLNAFIFLIAGLIALIITNRITNSFSLISDKMKEVNLGKQNEAIAWNRNDEI
                     GELVQEYNKMVAKLGESASALAKSEREGAWREMARQVAHEIKNPLTPMKLSIQYLQKA
                     INNNQENVKELSASVANTLVEQIDHLSKIAADFSQFANIGNVRAELFDLHDVISSLRD
                     LYLSNQQVEFICYPLLEKAMVNADKTQMNRLFTNLFTNAVEACRENGNCRIEVN"
     assembly_gap    3120..3179
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3180..>3407)
                     /locus_tag="PK977_17355"
     CDS             complement(<3180..>3407)
                     /locus_tag="PK977_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19949.1"
                     /translation="SRSSVTQEAKKAESYLHRLQKDFNSVLADTGLINRLLSRTSPLQ
                     EFTSLATKPYGIFLYSRDDFENTRMLFWNDQL"
BASE COUNT         1047 a          709 c          632 g          959 t
ORIGIN      
        1 cattcacctc tatccggcaa tttccgtttt cccggcaggc ttctacggca ttggtgaaga
       61 ggttagtaaa gagacggttc atctgggttt tgtcagcatt caccattgct ttctctaata
      121 atggatagca gataaattct acttgctggt tggaaaggta aagatcccga agcgaactaa
      181 tgacatcatg caggtcaaac agttctgcac gaacattgcc gatattggca aactgggaaa
      241 aatcagcagc aatctttgac aagtgatcaa tctgttccac taatgtatta gccacactgg
      301 cagaaagttc ttttacattc tcctggttat tgttgatggc tttctgcagg tactggatac
      361 tcaatttcat cggcgttagc ggattcttaa tctcatgggc tacctgacgg gccatttccc
      421 gccacgcccc ttccctttca ctcttggcca gtgcagaagc actctcccct aatttagcca
      481 ccattttatt atactcctgt accagttctc ctatttcatc atttcggttc catgcaatgg
      541 cttcattctg cttgccaaga tttacttctt tcatcttgtc gctgatcagg gagaatgaat
      601 tggtaatacg gttagtgatg atcaacgcaa ttaacccggc tataaggaaa ataaaagcat
      661 tcaggttaat gatggtaaca aggaagttgg aaatttcctg tcggagttcc ggctttgaag
      721 tgaaataagg aatattgaca aatgcttcta cttctcctag ttcgttgcgt accggcgagt
      781 agatactcag aaatgaaaaa ttccccactt tttcttcctg tacatgctgc acctgcctta
      841 accggctcag atggaaataa gcccttggct ccattttttt gcttaacact cctttggtgt
      901 aaacattcgc ctccgatgaa acctgaagat tgccatccag gtcataaaca tttacatcca
      961 caccatgtat atctgatact tcatccacca gtttttgcag tccgttattg gaaacagaat
     1021 catatatctt cactacatca tcaaaagtat tgtgatccga cattttcttt tccatttcat
     1081 ttaccatgat cttcatggta cggctaagtt tgtcgttatt actttgccgg ttccggctta
     1141 tgaaaaaaga aatggtggca gcccctatta ccaggaatga aaatacactg atgaaaatga
     1201 tggtactgtg tacctgtgac cgtatattga tctggaaaag tttccggaaa ccctgccagt
     1261 tagtacccgt tcttactatt agcgaaagaa actggagcaa tgcaacaagg aaaaggaaag
     1321 aacaaaagat ataagaaaac aaagtaatcg tttcaatagt tgtttcccgt ttacgggcaa
     1381 taactactac tttctcttta ctggccctgt accagagttc atcatagtcg ccattcgttt
     1441 ttagccggta ctcttctttg ggaatctcct catcattcag ccaggtggca aatggataat
     1501 tgccgggagg tgacaccagc ctgttcttta tataaaccgc attcgaatag ataggcgaat
     1561 tttcaggatc agtgcctttg ctctgccgga ataattccgg gaacaacgca tcacgactaa
     1621 aatttttcaa attagaaaca atgaaaaaag aacccatttt ctttcctgaa gtatccttta
     1681 cctcccggcg gatgatataa ttaaacttat cgaatgaagt ttcataataa tacaggccct
     1741 ctgttgctgt gggttttgat tgaacggtaa agattacatt cagtgcttca taactggtgg
     1801 gatcttcgtt aaaaagtgcc cggttggcag aatcataaac atatagccgg gtatcatact
     1861 tatttaaaaa accgctgtaa tttcccgtaa tgatactatc ctttaaggcc tgaccttctt
     1921 cctccttgtg aaaacgatga aagttttcgc tcagaaaatc attatccaag tactttacag
     1981 ctatactcat catcatttca ctggacggat cggtctgaac agccagcttt tcagcgatgg
     2041 attttctttt ctcccattca accctggagt tctgagacag cataatcgca gcaatcgatg
     2101 ctgaaaagat gaaaatccat gttaatatac ttgcaatatt aatcctgatg cggttaagga
     2161 aaacgccttt actattaatt aaccaggcat agattagcaa ccatatgagc accgggagat
     2221 aaaataacac ttcaggatta ccagaccttg ttgtcaggta taacaaacca gcaaaaccaa
     2281 tgataaagta aaccatgaac cgccgccccg caagcaatgg aaaaataagc ctgaatagca
     2341 actgagagaa atagtagtaa gacaaagaaa gacaagcaag cacaaaaaaa cctgctacag
     2401 tatattggtt gagactgaaa aaattggtta catcaaaaga aatcttggaa tctgcaacca
     2461 aactccggat aactgacgcc agtataaatg ttgaaaaaat taaaaacacc aatgccagca
     2521 agccagcaat taactttgag cgaaaagaaa aatcctttga aagattttct tcgcttttca
     2581 actttgacca ggcaaataaa accaaccagc aaaacaggat ggaattaatc accaggtcgc
     2641 ctaaagatcg ctgaatagcg ttggagccat atataagcgg actaaaaaac tcaaactgac
     2701 gcaggtttaa taatgccggg tataaatagc tgatcagtct tgtagctaat aataatacac
     2761 ctaaaagtcc aattgctttc caggccccat ttttccggga aaatgactca gtatatctat
     2821 agataaaaat aaaaagcaat agtatggcag cgagtcgtaa gtaaatcgta aggttatcat
     2881 tgtaaggaac agcgttagca acctttttac caagatagaa aacagcattg cctgttatag
     2941 acctgacagg aaagtctgta acatctttac ttatccgggc tcttttatct gcagttttgc
     3001 tgaagaaaaa gtgttcgggc aggtaatcag tctcaatgaa aaattcagaa cgaacaggta
     3061 tcaatgcaaa ggcgagcacc tttccctgtt ttaaaagcaa gctttttttt gtaataagan
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3181 agttgatcat tccagaacag catccgggta ttttcaaaat catctcttga gtaaaggaat
     3241 ataccataag gttttgtagc aagcgatgta aattcctgca gaggacttgt cctgctcaac
     3301 aaccggttta tcaaaccagt atcagcaagc actgaattaa aatccttttg aagacgatgc
     3361 aggtaacttt cagctttttt agcttcctgt gttactgaag aacggct
//
LOCUS       DAORLS010000320         3353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15493_length_3353_cov_1.919951, whole genome shotgun sequence.
ACCESSION   DAORLS010000320 DAORLS010000000
VERSION     DAORLS010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15493_length_3353_cov_1.919951"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>727)
                     /locus_tag="PK977_17360"
     CDS             complement(<1..>727)
                     /locus_tag="PK977_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF19950.1"
                     /translation="QVKGAIEFEKVWFAYTDDNYVLKDVSFKANPGETIAIVGHTGSG
                     KTTIISLLNRLYHIQKGTIKIDDINIEEFELECLRKNIGVVLQDVFLFSGSVMDNITL
                     RNPEIPGEKVMEAAKLIDMHDFIMRLPGGYDYNVMERGATLSLGQRQLLSFIRAMLYN
                     PSVLILDEATSSVDTESEQLIQHAIDTLIKGRTSIIIAHRLSTIRKANKIIVLDKGEV
                     VEAGTHDELLKKGGFYSKLHEMQF"
     assembly_gap    728..741
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<742..>1094)
                     /locus_tag="PK977_17365"
     CDS             complement(<742..>1094)
                     /locus_tag="PK977_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF19951.1"
                     /translation="VVQAFAAENKEFEKFKKINKEHRNANIKAIFAYSVFFPVVEIVL
                     ALSIGLIVWYGAGQRAEAGTIISFILCLNLLFRPLRVIADKFNVLQMGIIASERVFKV
                     LDNEDFADNNGTHAPA"
     assembly_gap    1095..1173
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1174..1741)
                     /locus_tag="PK977_17370"
     CDS             complement(<1174..1741)
                     /locus_tag="PK977_17370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF19952.1"
                     /translation="MPENKKSFFDFGLLRRVFGYAAPYKKKFYLSVVLAILLAVITPV
                     RPLLIQVTVNKYIANSLADMVVKVTLIQIGLILVETAMRFLFSLTTAVLGQSVVKDMR
                     IATYKKILNLNLSQFDKTPIGTLTTRTINDIESINDIFSDGLVPIIADMLSIVCVLVF
                     MFWSDWQLTLIALIPFPIMLIATYYFKES"
     gene            complement(1786..>1997)
                     /locus_tag="PK977_17375"
     CDS             complement(1786..>1997)
                     /locus_tag="PK977_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19953.1"
                     /translation="EVNRPFFQLLTEKVRNGNSIDWVPFWKELDRMVTAKEFEDYIPP
                     YKNLGALFIKAYKIAMGEEEEKKGF"
     assembly_gap    1998..2034
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2035..>3353)
                     /locus_tag="PK977_17380"
     CDS             complement(<2035..>3353)
                     /locus_tag="PK977_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19954.1"
                     /translation="KYARQLYDFSQANNHLGYVIRNITDDVFIKTIVKMARSRSGQQY
                     FPFLDNIVSGKMTIEDIDAVKDDSLQYYKLLVRTQMDYVARAIEKDTAFEYKSLTDRL
                     EKKAREDFVNVINGLHNEGAEVRFRSIQPLSAEELYYLAVSSDGSIYTSSFVKGVYPL
                     MMKKVNNRGDSLLMKLKFDKYRKFIKMSAGYNTLGNFLSSFPKTDDAASLMRAFVSNL
                     EKSDGLEDGVDVADSYASIAETIKPLAEEMLANVERNYERNIAAGNKKGIAIYNILQK
                     LFLSADSTKKVDLTKELGIPPVYEVPFNAMVNDSGRVIMQVFFYGDKDGQGIFVGFQN
                     MYGNANWKIDRSNPQWITINSVKGRPVTIFANRPLPEETGEDEKAQQALGEYLEKNKL
                     YPTITIHRGHSYYAPYTIAQMFPTSKIVFLGSCGGYHLVHDVLAKAS"
BASE COUNT          900 a          745 c          606 g          972 t
ORIGIN      
        1 caaactgcat ttcatgcaat ttactataaa aaccaccctt tttcaacagt tcatcatgag
       61 tgccggcctc tactacttct cctttgtcga gcactatgat tttatttgcc tttcggatgg
      121 ttgacaggcg atgggcaata ataatcgagg ttcttccttt gattaaggtg tcaatggcat
      181 gctgtatcag ttgttcgctt tctgtatcta cggatgaagt tgcttcatcc agaatcaaaa
      241 cagatgggtt gtacaacatt gccctaataa aagacaataa ttgacgctgg cccagagaca
      301 gtgtggcccc cctttccata acattataat catatcctcc tggcaaccgc atgataaaat
      361 catgcatatc tatcagtttt gctgcctcca taaccttttc ccctggtatt tcaggatttc
      421 gcaaagtgat attgtccata acagaccccg agaacaaaaa aacatcctgc aacaccaccc
      481 cgatattttt gcgcaggcat tccagctcaa actcttcaat atttatatca tctattttaa
      541 tggttccttt ctgaatatgg tacaacctgt tgaggaggct gatgatggtg gttttaccgc
      601 taccggtatg gccgacgata gcaatcgttt caccgggatt cgctttgaaa gacacgtctt
      661 tcaatacata attatcatca gtataagcaa accagacttt ttcaaactca atggcgccct
      721 ttacctgnnn nnnnnnnnnn ngccggtgca tgggttccgt tattgtcggc gaagtcttca
      781 ttatccaata ccttaaacac cctttcactg gcaataatac ccatctgcag tacattaaat
      841 ttgtctgcaa taacacgaag tggacgaaat aacaggttca ggcaaagaat aaaggagata
      901 atagtccccg cctcggccct ttgcccggcg ccataccaca caataaggcc gatagataaa
      961 gctagtacga tttcgaccac cggaaaaaaa acagaataag cgaaaatggc tttaatatta
     1021 gcgttccggt gttctttgtt gatctttttg aatttttcaa actccttatt ctctgctgcg
     1081 aaagcctgta ccacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgctttc tttaaaataa taggtagcaa
     1201 tcaacatgat cggaaaaggt ataagtgcta tcagcgtcag ttgccagtcg gaccaaaaca
     1261 tgaacaccag cacgcaaacg atggaaagca tatctgcgat aattgggacg aggccgtctg
     1321 aaaaaatatc attgattgat tcaatatcgt taatagttct tgtggtaaga gttccaattg
     1381 gtgtcttgtc gaactgggaa agattcaggt taaggatttt cttataggta gcaatacgca
     1441 tatcctttac caccgactgg cccaataccg ctgtggtcaa cgaaaacaga aaccgcatgg
     1501 ctgtttccac caatatcagc ccgatttgaa taagggtaac cttcactacc atatctgcca
     1561 gtgaattagc gatatactta ttcaccgtca cttgtataag caagggcctg acaggtgtga
     1621 tcacggccaa aaggatagcc agcactacgg acaggtaaaa tttctttttg tagggagccg
     1681 catatccaaa cactctccgc aacaacccaa aatcgaaaaa gcttttctta ttttctggca
     1741 aaatgaacag ttaatgaagg ggcaaaatta accagaaggg aatgattaaa atcctttctt
     1801 ttcttcttcc tcgcccatag caatcttata cgccttgatg aataaagcac caaggttttt
     1861 gtaagggggt atataatctt caaactcttt agctgttacc attctgtcca gttctttcca
     1921 gaacggcacc cagtcaatac tattcccgtt ccttactttt tctgtcagaa gctgaaagaa
     1981 aggacgattt acttctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggatg
     2041 cttttgccag cacatcatgt accaggtgat acccgccgca tgatcccaga aatacaattt
     2101 ttgatgtagg gaacatctgg gcaattgtat aaggagcata gtagctatgg ccccggtgta
     2161 tagttatggt agggtacaat ttatttttct ccaggtactc tccaagggct tgttgggctt
     2221 tttcatcttc acccgtttct tccggcaatg gacggttggc aaaaatggtg accggccttc
     2281 cttttactga attaattgtt atccattgcg gattgctcct gtctatcttc cagttggcat
     2341 taccatacat gttctgaaag cccacaaata ttccctgccc gtctttatca ccgtaaaaaa
     2401 atacctgcat aatcaccctg ccgctatcgt tgaccatggc attaaaaggg acttcataca
     2461 caggcggtat tcccaattct ttggtcagat ctactttttt ggtagaatct gctgaaagga
     2521 acagcttctg aagaatgtta taaatcgcaa tgcctttctt attgccggcg gctatgtttc
     2581 gttcataatt cctttccaca ttggccagca tctcttcggc aagaggtttt attgtttcag
     2641 caatgctggc ataagagtct gccacatcaa caccatcttc caggccatcc gatttttcaa
     2701 gattgctgac aaaagccctc atcaggctgg ctgcatcatc tgttttgggg aatgagctta
     2761 agaagtttcc cagggtatta tagccggcag acattttgat aaacttcctg tatttatcaa
     2821 actttagctt catcagcaat gaatcgcccc tgttatttac ctttttcatc atcagcggat
     2881 atactccttt cacaaaactt gaggtgtaaa tggatccatc ggaagaaaca gcgagataat
     2941 acaattcctc tgccgagagt ggctggattg acctgaaccg aacttcagcg ccttcgttgt
     3001 gcaggccatt gatcacattc acaaaatcct ctcttgcttt tttctccaac ctgtcagtaa
     3061 gtgatttgta ctcaaacgct gtatcttttt caatagccct ggccacataa tccatttgtg
     3121 ttctgaccag cagtttataa tactgcaggg aatcatcttt cactgcatca atatcttcaa
     3181 tagtcatttt accgctgaca atgttatcca aaaaagggaa atactgctgg ccacttctgc
     3241 ttcgggccat ttttactatt gttttgatga atacatcatc tgtaatattc ctgatcacat
     3301 agccgagatg attattggct tgcgaaaaat catacaactg ccttgcatat ttt
//
LOCUS       DAORLS010000321         3351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15503_length_3351_cov_2.236954, whole genome shotgun sequence.
ACCESSION   DAORLS010000321 DAORLS010000000
VERSION     DAORLS010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15503_length_3351_cov_2.236954"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>380
                     /locus_tag="PK977_17385"
     CDS             136..>380
                     /locus_tag="PK977_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF19955.1"
                     /translation="MANVNLRDSFNLLSKLTLRRAWNGVKVLSSYFISKWTKKPVQWG
                     YPVSISFEPTTSCNLRCPECPSGLRAFTRPTGMLQKD"
     assembly_gap    381..484
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <485..1174
                     /locus_tag="PK977_17390"
     CDS             <485..1174
                     /locus_tag="PK977_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRF19956.1"
                     /translation="MVKYASQKKIYTATSTNAHYLTDENAKRTIESGLDRLIISIDGT
                     TQDVYQQYRVGGQLQKVIEGATNIVKWKKELKSKKPFVVFQFLVVKPNEHQVEDVKRL
                     AKEIGVDDVWFKTAQVYDYKNDPNQLIPVTDKYSRYKKTADGYIFKGKLANHCWRLWH
                     DPVITWDGLVAPCCFDKDAQHQLGDLKKKSFKEIWKNGEYTKFRKQILQGRKNIDICS
                     NCSEGTKVWER"
     gene            1190..1672
                     /locus_tag="PK977_17395"
     CDS             1190..1672
                     /locus_tag="PK977_17395"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF19957.1"
                     /translation="MQLSSAISTRLHYQHKVLVEILDGLSDEQIRRQLNPGKWSIFEN
                     VVHLQTFQHKFISRVKQILKNEEPVFDRYTAEGDPLFLDNCHKSTREIMQDLLTTRKE
                     MTAEIVSFKETDFDKRGTHIVYGKMNLHQWLNFFLLHEAHHLFTIFKLAAELKQADRT
                     "
     gene            1989..2342
                     /locus_tag="PK977_17400"
     CDS             1989..2342
                     /locus_tag="PK977_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF19958.1"
                     /translation="MSDLRTNVDQLNQMVLEGKILDAFEKFYDDNIVMQDNDYPARVG
                     KDVNRQYEEAFVNGLTEFRGAKILNTLVSDDLAVTEWWFDYTHKDYGVRSYRQLAVQR
                     WKNGKIVEEKFYYNN"
     gene            2421..2879
                     /locus_tag="PK977_17405"
     CDS             2421..2879
                     /locus_tag="PK977_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRF19959.1"
                     /translation="MGIEKDIQQAKFRNAHQKAAINLIYTLSWIKERTKCIFETEDIT
                     SQQFNILRILRGSFPQPLSTLQIRERMLEKMSDTSRIVDRLIAKGLVKKVICKNDRRL
                     VDVIITDKGKKLLERLDTRQDEIDGVLGNLSEKDANTLSDLLDKIRDGGE"
     gene            2918..>3351
                     /locus_tag="PK977_17410"
     CDS             2918..>3351
                     /locus_tag="PK977_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRF19960.1"
                     /translation="MNRSHWLLLLALITFQISVAQPKYEFRGVWIATVNNIDWPPAGV
                     TDPEKQKADYIRLLDLHQKNGMNAMIVQVRPAADAFYPSQYEPWSQWLTGVQGQPPSP
                     YWDPMQFMIEETHKRGMEFHAWLNPYRAVHNTASSSIAGNHIT"
BASE COUNT         1013 a          656 c          724 g          854 t
ORIGIN      
        1 ccgtcaaaaa attacctgct tggctctaaa gtattaaacg gctgattgga cacacaacaa
       61 ggatggatga ttacaaaagg cccaactcac cctatggttg ggcttttttg ttttcaggag
      121 cttaacttta ctgcgatggc taacgttaac ctgcgggaca gttttaattt attatccaaa
      181 ctcactttac gccgggcatg gaacggggtg aaagtgttat cgagttactt tatcagcaag
      241 tggacaaaaa agccggtgca gtggggctat cctgtttcta tttcttttga accgactact
      301 tcctgtaatc ttcgctgtcc ggagtgcccc agcggattaa gggcttttac aagaccaacc
      361 gggatgctgc aaaaggattt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnatggta aaatatgctt cacaaaagaa aatatatacg gctacctcca ccaatgcgca
      541 ttatttaact gatgaaaatg cgaaacgtac catcgaaagc ggacttgatc gtctcattat
      601 ttcaatagac ggcacaacac aggatgtata ccagcagtac agggtgggcg gccaactgca
      661 aaaagtaata gaaggagcca ccaatatagt aaaatggaaa aaggagctta agagcaaaaa
      721 gccatttgtg gtgttccagt ttctggtagt aaaacccaac gagcaccagg tagaagatgt
      781 aaaaaggctt gccaaagaaa taggggtgga tgatgtgtgg ttcaagacag cacaggtata
      841 tgattataaa aatgatccga accaattgat accggtaact gacaaataca gccggtataa
      901 aaaaactgct gatggctata tctttaaagg aaagctggcc aatcactgct ggcgactctg
      961 gcatgatcct gtcattacct gggatggcct ggtggcaccc tgttgtttcg ataaagatgc
     1021 tcagcatcag ctcggcgacc tgaagaaaaa atcgtttaaa gagatttgga aaaacgggga
     1081 atacactaaa ttcaggaaac aaattttaca gggaagaaag aatattgata tctgctctaa
     1141 ttgtagcgaa ggaacaaaag tgtgggagcg gtaatattaa atcatacata tgcaattatc
     1201 ctctgctata tcgacacgac ttcattacca gcataaggtt ttggtagaga ttcttgatgg
     1261 tttgagtgat gaacaaataa gaagacagct caatcccggc aaatggtcca ttttcgaaaa
     1321 tgtagttcac cttcaaactt ttcagcacaa gtttatcagc agggtaaagc aaatattaaa
     1381 aaatgaagag ccggtttttg accggtacac tgctgaagga gacccccttt ttttggataa
     1441 ctgccataaa tccacccgtg aaattatgca ggacctgctg acgacaagaa aggagatgac
     1501 cgccgagatt gtttccttca aagaaacaga ttttgacaaa agaggtactc atatagtgta
     1561 tgggaaaatg aacctgcacc agtggctgaa tttcttttta ctgcatgagg cacatcacct
     1621 cttcactatt tttaagctgg ccgcagagct taaacaggca gaccggacat aagggggtgc
     1681 ttaccatttc tgcacttccc gatatagaga agtgtagaat gaagtgaatc cttcaaaaaa
     1741 gctgcgtatt gattctgata aaattttcat ggtggttata ttttaagtga ctttatccta
     1801 ctgtaaagct gcagttttcc cgtctttccg acaataccac aaagaggtga ttttttacac
     1861 cggtgctgcc cgtcaaatgt taataaatgg ctaagtctgt ccaagcggga aaataaattt
     1921 tgataaatca atgttgcaac attaaatttg cactttcaat tattttatca cacactaaaa
     1981 cttataacat gagcgattta agaacaaatg ttgatcagct gaaccaaatg gtgctggaag
     2041 gaaaaatcct ggacgctttt gaaaaattct atgatgataa catcgtaatg caggacaacg
     2101 actatcctgc ccgggtaggc aaggatgtaa accgccagta tgaagaagct ttcgtgaacg
     2161 gcctgacaga attccgtggt gccaagattc taaacaccct ggtgagtgat gacctggccg
     2221 ttacagagtg gtggtttgat tatacccaca aggattacgg cgttcgtagc tatcgtcagc
     2281 tggctgttca gcgctggaag aacggtaaaa ttgtagaaga gaaattctac tataataact
     2341 aagctatata accgtaccgg atgaatattg tttcgggtta agtgttctgc ttagcccgaa
     2401 actttttact tttaccaaag atgggaatag agaaagacat acaacaggca aaatttcgta
     2461 atgcgcatca gaaagcagcc atcaatctta tctacacgtt gtcgtggata aaggaaagga
     2521 caaaatgcat tttcgagacc gaagatatta cgtcccagca attcaatatt cttcggattc
     2581 tccgtggcag ttttccccag cctctatcca ccctgcagat cagggagcga atgctggaaa
     2641 aaatgagtga taccagccga atcgttgacc ggctgatcgc aaagggactt gttaaaaaag
     2701 tgatttgtaa gaatgaccgt cgtttggtag atgttatcat taccgataaa ggcaaaaaat
     2761 tattagaaag gttagatacc cggcaagacg aaattgacgg cgtattgggt aatctttctg
     2821 aaaaagatgc taatacttta agcgatctgc tggataagat cagggatggc ggcgaatgat
     2881 gtaaatttgc ccgattgttc atcccgaatc tgaaaacatg aatcgttccc actggttatt
     2941 gcttttagcc cttataacat ttcaaatttc tgtagctcaa cccaagtatg aatttcgtgg
     3001 cgtatggatc gctacggtca ataatattga ctggccacct gccggagtga ctgaccctga
     3061 aaaacaaaag gccgattaca tccgcctgct ggatctgcat cagaaaaatg gcatgaatgc
     3121 catgattgtg caggtgcggc ctgctgccga tgctttttac ccctctcaat atgagccctg
     3181 gagccagtgg cttacaggtg tgcagggcca gcccccctct ccctactggg atccgatgca
     3241 gttcatgatc gaagaaaccc ataaacgggg aatggaattt catgcctggc tgaatcctta
     3301 ccgggctgtt cacaatacag ccagttcttc catcgccgga aatcatatca c
//
LOCUS       DAORLS010000322         3349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15517_length_3349_cov_2.486035, whole genome shotgun sequence.
ACCESSION   DAORLS010000322 DAORLS010000000
VERSION     DAORLS010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15517_length_3349_cov_2.486035"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /gene="rpsP"
                     /locus_tag="PK977_17415"
     CDS             complement(<1..265)
                     /gene="rpsP"
                     /locus_tag="PK977_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRF19961.1"
                     /translation="MSAKIRLQRHGSKKRPFYFIVVADSRAPRDGKFIQKLGTYNPLT
                     IPATIQLDRQKALEWLNKGATPTDTVRRILSFKGVLYLKHLLRG"
     gene            complement(440..1765)
                     /gene="ffh"
                     /locus_tag="PK977_17420"
     CDS             complement(440..1765)
                     /gene="ffh"
                     /locus_tag="PK977_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRF19962.1"
                     /translation="MFNSLQEKLESAFKNLKGQGRITELNIASTVKDIRRALVDADVN
                     YKIAKEFTDKVKDKALGEKVLNAISPGQLMVKIVKDELTELMGGQEAPFNAKGNPAVI
                     LIAGLQGSGKTTFSGKLANYLKSKKGLSPMLVAADIYRPAAMEQLRVLGEQIGVDVHI
                     ELENKDAVDIARNAVKEARSKNKNVLIIDTAGRLAVDEVMMTEVANVKSAVNPQEILF
                     VVDSMTGQDAVNTAKAFNDRLDFTGVVLTKLDGDTRGGAALSIKYTVNKPIKFTSSGE
                     KMDTLDVFYPERMAQRILGMGDITSLVEKAQSAFDEEQAKKLEAKIRKNKFDFADFKM
                     QLEQIKKMGNMKDMLSMIPGVGSKVKDLDINEDSFKGIEAMINSMTPEERADPDLIDN
                     SRKKRIAKGSGKDINEVNAFMKQFEQMRDMMKNMNKMGAFGRMMPGMRK"
     gene            complement(1863..2732)
                     /locus_tag="PK977_17425"
     CDS             complement(1863..2732)
                     /locus_tag="PK977_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRF19963.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVELITPEEEVKLATLIK
                     QGDQRALDRLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGLTNRIQKAYSLLEQQYEREPSAEELAE
                     VLEMDLEEVSSTLGINARHVSMDSPLSEGEENTLIDVLENPNAEKTDGELYHTESLKT
                     EIDRSLKTLTERQKEVICFFFGIGVDHPMSLEDIGEKFNLTRERVRQIKDKAITKLKT
                     SNRCKLLRTYLGA"
     gene            3049..>3349
                     /locus_tag="PK977_17430"
     CDS             3049..>3349
                     /locus_tag="PK977_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRF19964.1"
                     /translation="MSKQLYTLEFPVRCSPSILYEFLSTPAGLGEWFADKVDERDNIF
                     YFGWNGAFEKAEVVESEQDKFIRFRWLTAPKDEYFEFRIDKSEITNQTILLVKDFA"
BASE COUNT          856 a          736 c          697 g         1060 t
ORIGIN      
        1 caccacgaag caggtgcttc aggtataata cccctttgaa gctcaggata cggcgaacag
       61 tgtcagttgg tgtggcacct ttgttcagcc actcaagggc tttctgacga tccagctgaa
      121 tagtagctgg gatagtcagg gggttgtaag tacctaattt ctggatgaat ttgccatcac
      181 ggggagcccg ggaatcggct actacaatga agtagaaggg tcttttcttg gacccgtgtc
      241 tttgaagacg gatttttgct gacataaata gaaatttaac agcgttaaac aataattgtt
      301 agttccggta gaaaccggac ggcaaatata aggcggtcat cggagaatga caagtgaaaa
      361 ttggggacat atgggtctaa atactttgtg aaatagccca tacgacttta gacttcagac
      421 taataactaa aggctaacac tatttcctca tccccggcat catacggcca aatgctccca
      481 tcttgttcat attcttcatc atatcccgca tttgttcaaa ttgcttcata aaggcattta
      541 cttcattgat gtctttaccg gaaccttttg caatcctttt tttgcggctg ttgtcaatca
      601 ggtccgggtc ggctctttct tccggggtca tggagttgat catggcctca atgcctttga
      661 atgagtcttc gttgatatca agatctttta ccttgcttcc tacgccgggt atcatactca
      721 gcatgtcttt catgttcccc atcttcttga tctgctccag ttgcattttg aaatcagcaa
      781 aatcaaactt attctttcgg atcttggctt ccagtttttt ggcctgttct tcatcaaacg
      841 cagactgtgc tttttcaaca agtgaagtga tgtcgcccat acccaggata cgctgtgcca
      901 tcctttccgg gtagaacaca tccagggtgt ccattttttc accactgctg gtaaacttga
      961 tgggtttgtt aactgtgtac ttgatagaaa gcgctgcacc acctcttgta tcgccatcca
     1021 gtttggtcaa caccacaccg gtaaaatcaa ggcggtcgtt aaaggctttg gcagtattta
     1081 cagcgtcctg gccagtcatg ctatctacca caaacaggat ttcctgtgga tttactgcac
     1141 tttttacatt ggctacttcg gtcatcatca cctcatccac ggcaaggcgt ccggcagtat
     1201 ctatgatgag cacattttta tttttgcttc tcgcttcctt taccgcattc ctggcaatat
     1261 caactgcatc tttattttcc agctcaatat gaacgtctac gccaatttgc tcacccagca
     1321 ctctcaattg ctccattgcg gcaggccggt aaatatctgc agcaaccagc atgggggaaa
     1381 ggcctttttt cgatttcagg taattggcca gtttgccgct aaaagtagtt ttaccggaac
     1441 cctgcaaacc ggcaatcagg ataactgcgg ggttgccttt ggcattgaaa ggggcttcct
     1501 gaccacccat cagttcggtc agttcatctt ttacaatttt taccattaac tgacccggag
     1561 aaatagcatt tagcactttt tcgcccaagg ctttatcctt caccttatcg gtaaattctt
     1621 tggcaatttt ataatttaca tccgcatcta ccagtgcacg gcggatgtct tttacggtag
     1681 aagcgatgtt aagttcagta atgcgtccct gacctttaag gttcttaaac gctgattcca
     1741 gtttttcttg taatgaattg aacatagttc acaaaagttt tcactgtcac acaaagaaaa
     1801 agtgaacatc aaatgttcac tttttttaaa gagatgcaaa gataagtagt gtacaagtta
     1861 tatcatgcgc ctaaataggt tctaagtagt ttacaacgat tggatgtttt tagtttggta
     1921 atggccttgt ctttaatctg ccttaccctt tccctggtca ggttaaattt ttcgccaata
     1981 tcttccaggc tcatgggatg atccacgcca ataccaaaga agaagcaaat tacttccttt
     2041 tgcctttccg tcagggtttt aagactgcgg tctatctcgg ttttgagcga ttcggtgtga
     2101 tatagttcac cgtcagtttt ttccgcattg gggttttcaa gcacatcaat tagcgtattt
     2161 tcttcgcctt ctgaaagggg agagtccatg cttacatgac gggcattaat accaagggta
     2221 gaggagactt cctcaaggtc catttccaat acttcagcca gctcttcagc cgaaggttct
     2281 ctttcatact gctgctccag cagagagtag gccttctgaa tccggttggt gaggcccact
     2341 ttattgagtg gcagtcttac aattctcgat tgctcggcca gtgcctgtaa aatgctctgg
     2401 cgaatccacc aaacggcata ggaaatgaac ttgaatcccc gggtttcatc aaaacgctgg
     2461 gcagccttga taaggcccag gtttccttca ttgatcaggt ctggcagcga caaaccctgg
     2521 ttttggtatt gtttggcaac agatactaca aaacgaagat tggccttggt caaccggtcc
     2581 aatgcccttt gatctccctg cttaataagg gtggctagtt ttacctcttc ctccggggta
     2641 atgagctcga ctttaccgat ctcctggagg tacttttcaa ggctttgaga ttcacgattc
     2701 gtaatagact tcgtgatctt gagttgtctc atgctcatag gtatggtttt tagaatggtg
     2761 tattttaagg gttaaagaat tataagtttt ggtattgcct tatactctaa aaatacccac
     2821 cacaaaatct tagttgctca gaaagtattg aacttcaaag caatttatgg aaattgccta
     2881 ttccggcaaa ataaattttg acatctcatt aacgttttca gaccttgttt atttcacagc
     2941 ttttgaggtt aggaccagtt gagggaaaaa taaaccataa aatattttgc cgcaatgatt
     3001 tattttctat tattgcagcc atcgcatcaa ttttttgatt taagaataat gagtaaacaa
     3061 ttatacacct tagagttccc ggttcgttgc tcgccgtcca ttttgtacga gtttttatca
     3121 acccctgcgg gtttggggga atggtttgcc gataaagtag acgaaagaga taatattttc
     3181 tattttggct ggaatggcgc ttttgagaaa gcagaagtag tggaaagtga gcaggataag
     3241 ttcattcgtt ttcgctggct cactgcccct aaagatgaat attttgaatt ccgcattgat
     3301 aaatctgaga tcaccaatca aacaattctg ttagtaaagg actttgcag
//
LOCUS       DAORLS010000323         3349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15520_length_3349_cov_1.450820, whole genome shotgun sequence.
ACCESSION   DAORLS010000323 DAORLS010000000
VERSION     DAORLS010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15520_length_3349_cov_1.450820"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>751)
                     /locus_tag="PK977_17435"
     CDS             complement(<1..>751)
                     /locus_tag="PK977_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRF19965.1"
                     /translation="LVKDAGGTDKFVGIGMGAPNGNFYTGSIDYAPNLKWKGIIPLAS
                     MINERFGLPAKLTNDANAAAVGEMMYGCARGMKHFIVITLGTGVGSGIVIDGKIVLGH
                     DGFAGELGHTIIRPGGRLHKGTGAKGSLEAYASATCVRETALELLTRYPEEESLLRNY
                     SINELTSQTVYECAIKGDKIANEIFEFTGEILGEALANFVMFSSPEAIILFGGLTKAG
                     DLIMNPTRQAMEANLIQVFKNKVKLLFSDLK"
     assembly_gap    752..765
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(922..>1551)
                     /locus_tag="PK977_17440"
     CDS             complement(922..>1551)
                     /locus_tag="PK977_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF19966.1"
                     /translation="EATPYIIMYWGSLMIGRWTGAISAFKLSDSTKNILRFVVPLVAF
                     GILIGINTAAQYDMKPLYFYVICVLIQIAAFYISKDKPARTMLLFSVLGIVAMVIGLM
                     TTGTVAIYAFLSGGLVCSIMWPCIFSLSIAGLGKYTSEGSAFLIMMILGGGIIPPIQG
                     KLADIIGIHHSYIVAAICFAYLAFFAFAVKGILKKQGIDYDAEVSAGGH"
     assembly_gap    1552..1682
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1683..1880)
                     /locus_tag="PK977_17445"
     CDS             complement(<1683..1880)
                     /locus_tag="PK977_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF19967.1"
                     /translation="MTGLLIVMFVPVFNSYKTDLTNAPEAERHAMEAYRLKWLIGALL
                     VVVVGLVIANLSAKKKADNWGP"
     assembly_gap    1981..2045
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2046..2688)
                     /locus_tag="PK977_17450"
     CDS             complement(<2046..2688)
                     /locus_tag="PK977_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19968.1"
                     /translation="MYKLSTALTGVFLPALKNVILSALFIKHSIAMSQPKQPTNYGAL
                     GTLVTVFFFWGFIAAGNSVFIPFCKHYFHLDQFQSQLIDFAFYTAYYIGALILFAYGA
                     YGGKDLVGKWGYKNSIVYGLLFSALGAAAMILAVNGNSFAGMLIGLFIVALGFSLQQT
                     AAQPFAISLGDPATGTSRVNLGGGVNSFGTSIGPLVVALALFGSTAAITDEKIA"
     gene            2813..>3349
                     /locus_tag="PK977_17455"
     CDS             2813..>3349
                     /locus_tag="PK977_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRF19969.1"
                     /translation="MEIIHVAAECYPVAKAGGLGDVVGALPKYQSELGHVAKVVMPMY
                     RTKFLYNNEWELVHEGNQHIGPHYFNYAVIKEKTNKLGFDLYLVDINGLLDREKIYGY
                     DDDTERFLAFQVAVVDWMNKWNHQPDVIHCHDHHTGLIPFMVKYCFEFRQKLANIPTV
                     FTVHNGQYQGWIGWDKYYL"
BASE COUNT          956 a          696 c          621 g          866 t
ORIGIN      
        1 tctttcagat cactgaatag cagcttcact ttattcttaa atacctggat caggtttgcc
       61 tccatagcct gccgggttgg gttcataata agatcaccgg ctttagtcag gcctccaaaa
      121 agaataatgg cttcaggaga ggagaacatg acaaaattag ccagggcttc ccctaaaatt
      181 tccccggtaa actcaaatat ttcattggca attttatcac ccttgatagc acattcataa
      241 acggtctggc tggttagttc attgatggaa tagttgcgta acaaactctc ttcttcagga
      301 tagcgggtta ataattccag tgctgtttcc cttacacaag ttgcagaagc atacgcttca
      361 aggctgcctt tagccccggt tcctttatga agtctgccac cgggacgaat aattgtatgt
      421 cctagttctc cggcaaaacc atcatgaccg agtacaatct ttccgtcaat gacaataccg
      481 ctgcctacac ctgttcccag ggttataaca ataaagtgtt tcatgcccct ggcacagcca
      541 tacatcattt cacctacggc ggctgcattg gcatcattgg ttaatttagc cggcagacca
      601 aatcgctcat taatcatact ggccagcgga ataatacctt tccatttcag gttaggggca
      661 taatcaatag aaccggtgta aaaatttccg ttaggtgctc ccatgccaat accaacaaat
      721 ttgtcagtac cgcctgcatc tttgaccagc gnnnnnnnnn nnnnncaggt cttcaatgaa
      781 ctcctgtacg gtttcatgtt cattggtcag catcctgtcc tgtaccagta tatgcccgtt
      841 ccggtcaacc acaccaaatt ttgtattagt gccgccgata tctattccta ttgcaaattg
      901 agacatggtt ctttttttta attaatgacc acctgcactt acttcagcat cgtaatcaat
      961 accctgtttt ttcaaaattc cttttacagc aaaagcaaag aaggccagat aggcaaaaca
     1021 aatggcagca acaatataag aatgatgaat gccgattata tcggccagtt ttccctggat
     1081 aggaggaatg ataccaccac ctaaaatcat cattattaga aacgccgaac cttcagaagt
     1141 gtattttccc aaacctgcaa tcgataaact gaaaatgcat ggccacatga tggaacaaac
     1201 aagacctccg ctaagaaaag cgtagatggc aactgttcct gttgtcatca aaccaatcac
     1261 catggctaca attccgagca cactgaaaag taacattgtc ctggctggtt tatccttact
     1321 gatatagaaa gcagcaattt gtatcagcac acatattaca taaaagtaaa ggggcttcat
     1381 atcatattgc gctgctgtat taattccaat caatattcca aatgctacca gcggaacgac
     1441 aaaacgtaga atattctttg tgctatcgga caacttgaag gcactgattg cacccgtcca
     1501 gcggccgatc atcaggcttc cccagtacat aataatataa ggagtggctt cnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nncgggcccc agttgtctgc ttttttcttt gcgcttaaat tagcaatgac aagccccacc
     1741 actactacaa gcagtgcccc gatcagccat tttaaacgat aagcttccat cgcatgtcgt
     1801 tctgcctcag gagcattggt caaatcagtt ttataactat taaaaacagg tacaaacatt
     1861 acaatcaaca aacctgtcat gattaataaa gtatacagcg ccttactggc cttctctgta
     1921 gtctcttcca tcttgcctga aggtactttt ttggcgaaga aaaacatagc ggcggctgcc
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnaagca attttttcat cagtgatagc ggccgttgaa ccaaaaagag ctaaagccac
     2101 caccaaagga ccaatggatg taccaaatga attaacaccg ccacccaggt ttacccggct
     2161 ggtacctgta gcagggtctc ccaatgaaat agcgaaaggc tgggcagctg tttgttgaag
     2221 ggagaaccca agtgccacaa taaataaacc aattagcata ccggcaaaac tgtttccatt
     2281 cactgctaat atcatggcag cggcacctaa ggctgaaaag agtaatccat aaactatact
     2341 gtttttgtaa ccccattttc cgacaaggtc ttttcctcca taggctccat aggcaaataa
     2401 tataagtgcg ccgatataat aggctgtata aaaggcaaaa tcaatcagct ggctttgaaa
     2461 ctgatcgagg tgaaaataat gtttacagaa aggaataaaa acactgttgc cggcagcaat
     2521 gaaaccccaa aagaaaaaca cggtaacaag ggttcctaaa gcgccgtaat tggttggttg
     2581 tttgggttga ctcatagcaa tcgaatgttt aataaatagt gctgaaagta taacattttt
     2641 caatgccggt aaaaaaacac ctgttagtgc tgttgacagc ttgtacaaaa aaaagcggat
     2701 tcattttagg ggtaatgtaa atatgcttta aaatcacttt cttttgattg gtttatcata
     2761 catgctatga aaataatccc ctaaattacc gttcaaattc tgtgttataa taatggaaat
     2821 aattcatgtt gctgctgaat gttatcctgt ggcaaaagcc ggtggcctcg gagatgtagt
     2881 aggtgcttta cctaagtacc agtccgaact gggccatgtg gccaaagtgg tgatgccgat
     2941 gtaccgtacc aaatttttat ataacaatga atgggaactg gtgcatgagg gtaaccagca
     3001 tattgggccg cattatttca attatgctgt catcaaagaa aaaacaaata agctcggttt
     3061 tgatctttac ctcgttgata taaatgggct gctggacagg gagaaaatct atggatacga
     3121 tgatgatacg gaacgctttc ttgctttcca ggtagctgta gtggactgga tgaataaatg
     3181 gaatcaccaa ccggatgtta tacactgtca cgatcatcat acaggcctca tcccttttat
     3241 ggtgaagtac tgttttgaat tcaggcagaa actggctaat attccaacgg tttttacggt
     3301 tcacaacggg caataccagg gctggatagg ctgggataaa tattatttg
//
LOCUS       DAORLS010000324         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15729_length_3314_cov_2.085302, whole genome shotgun sequence.
ACCESSION   DAORLS010000324 DAORLS010000000
VERSION     DAORLS010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15729_length_3314_cov_2.085302"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2018)
                     /locus_tag="PK977_17460"
     CDS             complement(<1..>2018)
                     /locus_tag="PK977_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HRF19970.1"
                     /translation="SGLVNAPTGCGKTFSVLLGALIHFINQQGDNYKKVKNSGLQLLW
                     ITPLRALAKDIGRAMEEVITELGIQWKIGIRNGDTNTTERANQKKQMPEVLIITPESL
                     HLLLAQKSYPDIFRHLEIIAVDEWHELLGSKRGVQVELALSRIINCQWKLANKKPSVW
                     GISATIGNLAEAQEVLLSPLDIKGVTIKANLQKQIEVESIFPDEIEKYPWAGHLGIKL
                     AKKLIPIIQKSKTTLIFINTRGMSETWYQTLLDVCPDLAGSIALHHSSIDRELRTWIE
                     ENLHSGNLKAVVCTASLDLGVDFRPVETVIQVGSPKGVARFLQRAGRSGHSPDAISKI
                     YFLPTHSLELMEAAALKSAVSENMIESRPPMILCYDVLLQYMNTIAISDGFRSEETYR
                     EVVSTFCYKDLSLDEWQHMLHFITEGGNAMQQYDDFKKVELTDGIYKINSRKVAMRHR
                     MHIGTIVSDAMLKVKFMTGGYVGVIEEYFISRLDPGDVFTLAGRNLEFVMIKDMTVLV
                     RKSNAKKSIVPSWTGGRMPLSANLGKKLRQMLDITAMQNKLSNNDELNALWPLFNLQK
                     QLSHVPNENELLIEHIETKDGFHLFVYPFEGRLVHEAMAAILAWRISRIRPITFSFVM
                     NDYGFELLSDQPIPVDDTNVYELFSPDDLLNDIQRSVNSTEMAKRKFR"
     assembly_gap    2019..2114
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2177..>3314)
                     /locus_tag="PK977_17465"
     CDS             complement(2177..>3314)
                     /locus_tag="PK977_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRF19971.1"
                     /translation="AFQLVQAQNGTARHRIAIFAPLYLDSAFNELDEYRYAKNVFPKF
                     INPGLEFYEGAQLALDSLNKEYAQLEVFVYDTRSAKESLAEQLNKPEMDSMALIIAHC
                     NTSEVKQFADAGLKRNIPVINANLPNDGGTNGNPFFVLLNPTLRTQCEGIYRHIQKFY
                     SINPIIVFRKKGQLEDRIKLYFDEFGKNTVGVPLKMKYVDLTDSFTVNQLKPYLDTTN
                     QTLCVAGSLDENFGRRLTQQLAYLKKQKYLSSVVGMPTWDNIKDFNKPEYKGLEITYS
                     NPFYNAKLDKFSQSIINHFNNVMYARPSDMVFRGYEVTWKYARLLLQYGKDLSSNLSN
                     KSHKVFTDFDIQPVVNKENMSLEYFENRKLYFLKWQDGVIKSIY"
BASE COUNT          853 a          684 c          657 g         1024 t
ORIGIN      
        1 cccggaattt ccgcttagcc atctcagtac tgtttacgct ccgctgaata tcattcagca
       61 ggtcatcggg tgaaaagagt tcgtaaacat tagtgtcatc aacagggatg ggctgatcac
      121 tgagtaattc aaacccataa tcgttcatga caaaagaaaa tgttataggc ctgatccggc
      181 tgatgcgcca ggcaagaata gccgccatag cttcgtgtac cagtcttccc tcaaaaggat
      241 aaacaaaaag gtgaaatccg tcttttgttt caatatgctc aatcagtaac tcgttttcat
      301 tgggaacatg agagagctgc ttttgcaggt tgaaaagtgg ccataatgca ttcaattcat
      361 cgttgtttga taacttgttt tgcatagcag taatatctag catctgccgg agtttttttc
      421 ccaggttagc acttaggggc attctgccac ctgtccagct tggaacgata gattttttag
      481 cgttgctctt tcgtaccagg actgtcatat ccttgatcat tacaaattca aggtttcggc
      541 cggcaagggt aaacacatct cccgggtcta gcctggaaat aaaatactcc tcgatcaccc
      601 cgacataacc acccgtcata aattttactt tcagcatggc atcgcttact atagtgccaa
      661 tatgcatacg atgtcgcatg gccactttgc ggctgttgat cttgtaaatg ccatctgtta
      721 attctacttt tttgaaatca tcatactgct gcatggcatt accaccttca gtaatgaaat
      781 gcagcatatg ctgccattca tctaatgaaa ggtccttata acagaaagtt gatacgactt
      841 cacggtaagt ttcttcagac ctaaacccat cagagatggc aattgtattc atgtattgaa
      901 gcaacacatc ataacacagt atcatgggtg gcctgctttc aatcatattt tcactgacgg
      961 ctgatttaag tgcagccgct tccataagtt cgagggagtg agtgggaaga aaataaatct
     1021 tgcttattgc gtccgggcta tggccactgc gtccggcccg ttgtaaaaac cgggcaacac
     1081 ctttgggtga acctacctgt ataaccgttt ctacaggccg gaaatcaacg ccgaggtcaa
     1141 ggcttgccgt gcaaacgact gcttttagat ttccggagtg taaattttct tcaatccagg
     1201 ttcttaattc ccggtcaata gaactgtggt gcagcgcaat ggaacctgcc aggtcgggac
     1261 aaacatccag caatgtctgg taccatgttt cactcatgcc cctcgtgttg atgaatatga
     1321 gtgttgtctt gcttttttga ataatgggga ttagtttttt cgccagtttt attcccaggt
     1381 ggcctgccca gggatatttt tcaatctcgt ccggaaaaat tgattcaacc tctatctgtt
     1441 tctgaagatt tgcttttatg gtaaccccct ttatgtctaa tggtgaaagc agcacttctt
     1501 gtgcttcagc caggtttcca atggtggctg aaatgcccca gacagaaggt ttcttgttgg
     1561 ctaatttcca ctgacaatta atgatcctgg ataaagccag ttctacctgt acccctcttt
     1621 tgctgccgag taattcatgc cattcatcaa cggctatgat ttcaagatgc ctgaaaatgt
     1681 ccgggtagct tttttgtgcg agcagcaaat gcaaactttc cggtgtaatg atcaatactt
     1741 cgggcatctg ttttttttga ttggcccttt ctgttgtatt ggtatctcca ttgcgtatgc
     1801 cgatcttcca ttgtattcca agctcagtta tcacttcttc catcgctctt ccaatatcct
     1861 ttgccaaagc tctgagcggg gtaatccaga gtaattgaag gcctgaattt tttacttttt
     1921 tataattgtc gccttgctga ttaatgaaat ggataagagc tcctaaaagg acagaaaatg
     1981 ttttgccaca acctgtgggg gcattcacca atccgcttnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnncctttt gtatcctgaa cattcacagt tacaaaaata aggggagcat
     2161 ttggagacag ctgaaattag taaatgcttt tgatcacccc atcctgccac ttcaggaagt
     2221 acaattttct gttttcaaag tattccagcg acatattttc tttattcacc acaggctgaa
     2281 tatcaaaatc ggtaaatact ttgtggcttt tgttagaaag attggaagag aggtcttttc
     2341 cgtattgtaa tagtaacctt gcatacttcc aggttacttc atagccccgg aaaaccatat
     2401 cgcttggccg tgcatacatt acgttattaa aatgattaat gatagactgg ctaaatttat
     2461 caagcttggc attataaaat gggttactgt atgtgatctc aagtccttta tactctggtt
     2521 tattgaagtc tttaatatta tcccaggtag gcattccaac tacggaggac aggtactttt
     2581 gtttttttag ataggctaat tgttgtgtca gtcttcttcc aaaattttca tccaggctac
     2641 cggccacaca taatgtttgg ttagtagtat caagataggg tttcaattgg ttaacagtga
     2701 aactgtcggt gaggtcaaca tatttcattt ttaacggtac cccaacggtg tttttgccaa
     2761 attcatcaaa gtataatttt atcctgtctt ccaactggcc ttttttgcgg aagactatta
     2821 taggatttat agagtaaaac ttctggatat gcctgtaaat gccttcacat tgggttctta
     2881 aggttgggtt aagaagtaca aaaaaaggat taccatttgt tccgccatca ttgggcaggt
     2941 ttgcatttat caccgggata tttcttttca acccggcatc tgcaaattgc ttaacctcag
     3001 aagtattgca atgtgctatt atcaacgcca tgctatccat ttccggttta tttagctgtt
     3061 cggccaggct ttctttggca gaccgggtgt cgtaaacaaa aacttccagt tgtgcatatt
     3121 ctttattcaa cgaatcgaga gccagttgag caccttcata aaactcgagg ccgggattaa
     3181 tgaattttgg aaatacattt ttggcataac ggtactcgtc aagttcattg aaagcggaat
     3241 caaggtacaa aggagcgaaa atggctattc tatgtctggc agtgccattt tgtgcctgaa
     3301 ccagttgaaa agct
//
LOCUS       DAORLS010000325         3313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15737_length_3313_cov_1.783917, whole genome shotgun sequence.
ACCESSION   DAORLS010000325 DAORLS010000000
VERSION     DAORLS010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15737_length_3313_cov_1.783917"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PK977_17470"
     CDS             complement(<1..626)
                     /locus_tag="PK977_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19972.1"
                     /translation="MSLLFLIACGSGKRSETESNIVVNSDTAVVNSLIRKANDFAQIN
                     FDSMHLYAEQAYKAASAADFPQGKARAKAVEANYQRRKGNYDKAISLGLEVMKSYDSL
                     GMKEESVRLKNIIADTYKEMGGEKGTKEFLSKGLELSRQAERDAEKMKYVAGVVISLN
                     QQGIILRDMSKRSDRKDLMDSALVLYQKGIKLVETTGEGEESLGKLYN"
     gene            complement(746..>1151)
                     /locus_tag="PK977_17475"
     CDS             complement(746..>1151)
                     /locus_tag="PK977_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HRF19973.1"
                     /translation="LSTEGGALKEFIIPLQFGIASILGSGRQVISWIHINDLVNMFIK
                     AMEDENINGVYNAVAPLPVSNKELILTLARTRNKFYIPFPVPSFVLKMILGEMSIEIL
                     KSATVSSAKAEKAGFSFRYANINSAISSFIKR"
     assembly_gap    1152..1279
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1280..1690)
                     /locus_tag="PK977_17480"
     CDS             complement(<1280..1690)
                     /locus_tag="PK977_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF19974.1"
                     /translation="MKTVLITGGTGMVGRALTKALLQKGYGVIVMGRGGVRDKGNGAG
                     EAGYETRDGVVGLSFAKWNVEKQEIDAAAIAKADYIVHLAGAGVADKRWTSKRKQEIV
                     DSRVKSGALLVHSLKSIPNKVKAVISASAIGWYGA"
     gene            1989..>2255
                     /locus_tag="PK977_17485"
     CDS             1989..>2255
                     /locus_tag="PK977_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF19975.1"
                     /translation="MSVVLSINNLSKSYGSVKALNGVSFDVPQGSVFGILGPNGSGKT
                     TLLGIVMDVLRANSGGFQWFGQPGTSPQLRQQIGSLLETPNFYSY"
     assembly_gap    2256..2280
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2281..2898
                     /locus_tag="PK977_17490"
     CDS             <2281..2898
                     /locus_tag="PK977_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF19976.1"
                     /translation="NLKITQSISRRGNKKEIDEVLKKVNLYNRRFSRFKEFSLGMKQR
                     LAIAAALLGDPKVLVLDEPTNGLDPVGIAEIRELIIELKDRGHTVIMASHLLDEVEKV
                     CTHAAILKTGQLIATGLVDEILMDEDVVEVSAANLPQLADLMRKLSDTVLLDEKAGTV
                     QLMLPKGTANMENINRLCFDNGIVLSQLMLKKKRLEARFFELTNN"
     gene            2987..>3313
                     /locus_tag="PK977_17495"
     CDS             2987..>3313
                     /locus_tag="PK977_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF19977.1"
                     /translation="MTNLIRTEWLKMKGYPAFWWILGATALAYPAINYGFYKIYEDIM
                     GNPTEAAQMAKMFIGNPFSFPEAWHTVAFASSLFTFIPAVVVIMFISNEYTYRTHRQN
                     IIDGWSR"
BASE COUNT          825 a          829 c          590 g          916 t
ORIGIN      
        1 ttattgtaca gttttcccag ggattcttcc ccttcaccag tcgtttccac cagctttatc
       61 cctttttgat ataataccag cgcagagtcc atcaggtctt ttcggtccga ccgtttgctc
      121 atgtccctta agatgatccc ctgttggttc aggctgatta ccactcctgc cacatatttc
      181 attttttcag catccctctc cgcctgtctg gacaattcca gtccttttga caaaaattcc
      241 tttgtaccct tttcaccgcc catttcttta taggtatctg caatgatatt tttaagccgc
      301 acagattcct ctttcattcc gagactgtca taactcttca tcacttccag gccaaggctg
      361 atggccttat cataattgcc ttttctacgc tggtaattag cttctaccgc tttggctctt
      421 gctttgcctt gcggaaaatc ggccgccgaa gctgccttat aagcctgttc agcataaaga
      481 tgcatggaat caaaattgat ctgggcaaaa tcgttcgcct ttctgatcaa tgaattgacc
      541 acagccgtat cgctgttaac tacaatatta ctttcagttt cacttctctt tccggagccg
      601 caggcgatca aaaaaagtaa ggacattacg gctaaaatat acccgtaaaa aaaacggtta
      661 cttaccgact ctaaaaatgg attcagccct gacatgagtg tgtaaaatag aaaataatgg
      721 ctgattcagg aaactacgtt cagctttacc gctttataaa gctgctgatc gctgagttta
      781 tatttgcata acggaaagaa aaccccgctt tctctgcttt ggctgagctt accgttgcac
      841 tttttagtat ttcaatgctc atttcgccca ggatcatttt caatacaaaa gaaggaactg
      901 gaaaaggtat atagaactta ttacgggtcc ttgccaatgt caagattaat tctttattag
      961 aaaccggtaa gggggccacg gcattatata ccccattaat attttcatcc tccatcgctt
     1021 taataaacat attcaccagg tcatttatat ggatccagct gatgacctgc ctgccgctgc
     1081 cgagaatgga ggcaatacca aactgcagag gtatgataaa ttctttcagt gcaccgccct
     1141 ctgttgacaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnc gcaccatacc acccaattgc tgatgcgctg atcaccgcct
     1321 tcactttgtt gggaatactt ttcaaactat gcaccagcaa tgcaccgctt ttcacccggc
     1381 tgtcgacaat ctcctgtttc ctttttgacg tccatctttt atccgccaca cctgcgcctg
     1441 ccaaatgaac aatataatct gctttagcaa tggcagcagc atctatctcc tgcttctcaa
     1501 cattccattt cgcaaacgac aacccgacaa caccatctct tgtctcgtac cctgcttccc
     1561 ctgccccgtt tcccttatcc cttaccccac ccctccccat tactatcact ccatatccct
     1621 tctgcagcaa agcttttgtc aatgcccgcc ccaccattcc agttccgcct gtaattaata
     1681 ctgtcttcat tttgtttatt gaagttagtc gctattctaa tatctgctct gattgaatac
     1741 ggtaaccaca gccttacaac gacaccgtga ttcacagagc ttttttctgc tacttcctgt
     1801 cttttagtta atacatcctc cctcagacat ccttgttacg cttcaccgga acaatctgag
     1861 ttaatccgtg ttcatctgtg gccaacttcc cttcctgcct ctgtggttta aaaacaacat
     1921 accattcacc atattgttct gccacattct ccataatctt ttaacttcac cgccataaat
     1981 tttatggcat gagtgtagtg ctctcaataa acaatctttc caaatcctac ggttctgtaa
     2041 aagcgctgaa cggcgtttcc tttgatgtac cccagggttc tgtattcggg atccttggcc
     2101 caaacggcag tggtaaaaca acactgcttg gcattgtcat ggatgtattg cgggccaaca
     2161 gcgggggctt tcaatggttt ggccagcctg gtacttctcc gcagttgcgc cagcagatcg
     2221 gctccctgct ggagacaccc aatttttaca gttatnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 aacctcaaaa tcacccagtc catcagccgg cgtggtaata aaaaagaaat tgatgaagtg
     2341 ctgaaaaaag tgaacctgta taatcgccgt ttcagccgtt ttaaagagtt tagtctcggt
     2401 atgaagcaac gcctcgccat tgctgcagca ttactcggcg atccgaaagt gctggtactc
     2461 gatgaaccca ctaacggtct cgacccagtt ggcattgctg aaataaggga actgatcatt
     2521 gaattaaaag acaggggcca tactgttatc atggccagcc acttgctgga tgaagtagaa
     2581 aaagtgtgta cccatgccgc catattaaaa acgggacagc ttattgccac cggcctggta
     2641 gatgaaatac tgatggatga agatgtggtg gaagtaagtg ctgccaacct gccgcaactg
     2701 gccgacctca tgcgcaaact cagcgatact gttttgctgg atgaaaaagc cggcaccgta
     2761 caattaatgt taccaaaggg cacggccaat atggagaata tcaaccgtct ttgttttgac
     2821 aatggcattg tgctctcgca actgatgctg aagaaaaaac gattagaagc aagatttttt
     2881 gaactaacta ataattaacc acactgtcac cctgagcctg tcgaagggtg aataaagtaa
     2941 tatgtctcat gcttcgacaa actcggcatg aaaaatacaa cgaactatga ccaatcttat
     3001 tcgcaccgaa tggctcaaga tgaaaggcta ccccgccttc tggtggatac ttggcgccac
     3061 tgccctcgcc tatcccgcta tcaattacgg gttttacaag atctatgaag atatcatggg
     3121 caaccctacc gaggcagccc agatggcaaa aatgtttatc ggtaatccct tctcttttcc
     3181 ggaagcatgg cataccgtgg cctttgcctc ttcactgttc accttcatac ctgctgtagt
     3241 tgtcatcatg ttcattagca atgaatatac ttaccgcacc caccggcaaa atataattga
     3301 tggctggagc cgc
//
LOCUS       DAORLS010000326         3293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_15860_length_3293_cov_1.899321, whole genome shotgun sequence.
ACCESSION   DAORLS010000326 DAORLS010000000
VERSION     DAORLS010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15860_length_3293_cov_1.899321"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK977_17500"
     CDS             <1..884
                     /locus_tag="PK977_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRF19978.1"
                     /translation="INYAATQPNIPFMSVKITGFARFALLEKMDEMMHQEQGTLMKRY
                     LAMLDKLSVEEREEWHRVRHRVMRVCDAAARNDVGVSIDAEETWITEPLDALTILMMD
                     TFNKERAVVYNTTQHYRTDRQQFLKDSYEAAVERNFILGAKLVRGAYMEKERKRAAER
                     GYPSPIMPDKESTDRDYNESVKFCIDHIDRIALMVASHNEYSNMYAAQLLQEKGLPLN
                     HPHVHWSQLYGMSDNITFNLAHAGCSVSKYLPFGPIKDVIKYLMRRAQENTSVKGQTG
                     RELSLIKKEVARRKIKA"
     gene            complement(881..1273)
                     /locus_tag="PK977_17505"
     CDS             complement(881..1273)
                     /locus_tag="PK977_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19979.1"
                     /translation="MLNSRRFYKELGNLFYAIAAADKKISPKEKKTLDDEVQFAWKHY
                     DNTTDRFGSDRAFLIEFEFETMEDNSETAETAYQAFETFFREKKNEIDEHTRARIFNS
                     ARHIAESVRKINHEELSYLVRLKQLLEL"
     gene            1430..2224
                     /locus_tag="PK977_17510"
     CDS             1430..2224
                     /locus_tag="PK977_17510"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRF19980.1"
                     /translation="MQQYHDLLRHILENGTDKKDRTGTGTRSVFGYQMRFNLQKGFPL
                     VTTKKVHLKSIIYELLWFLKGETNIAYLKEHGVSIWNEWADENGELGPVYGKQWRSWE
                     GKDGVVIDQITDLIKQIKNNPDSRRLIVSAWNVADLPRMALMPCHNMFQFYVAEGKLS
                     CQLYQRSADVFLGVPFNIASYALLTMMIAQVCDLEPGDFVHTFGDVHIYSNHMEQVNL
                     QLNRSPFPLPVMNLNPAVKDIFSFKFEDFTLENYQCHPAIKAPVAV"
     gene            2253..2747
                     /gene="folA"
                     /locus_tag="PK977_17515"
     CDS             2253..2747
                     /gene="folA"
                     /locus_tag="PK977_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HRF19981.1"
                     /translation="MIISLVVAAATNNAIGKDGKMPWHLPNDMKHFKNVTWGMPIVMG
                     RKTFDSLGKALPGRKNIVITRQHGWKADNTVAVKNIEDALFVSKETDAKEVMVIGGGE
                     IYKALFDKAQRIYLTRVEAEPVGDTFFPVLTQGQWHLVSQKNHEADEKNAYNYSFQVW
                     ERIG"
     gene            2766..>3293
                     /locus_tag="PK977_17520"
     CDS             2766..>3293
                     /locus_tag="PK977_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRF19982.1"
                     /translation="MYYAPYLALKYLRYYLTSSNGKGHGTHSPFIFDFITKVLNDNSP
                     YKEYKQAEGLRKRLLSDSRMFIVEDFGAGSAVLKEQERSIKSIARNAAKSKKYGQLLF
                     RIVKYYQPATILELGTSLGITTTYLSLANPASKIVTMEGAKEIAAIAKLNFRQLDISN
                     IELVEGNFDNTLPDVL"
BASE COUNT         1006 a          625 c          731 g          931 t
ORIGIN      
        1 tgattaatta tgccgctaca cagcccaata ttcccttcat gagtgtgaaa attaccggtt
       61 ttgcccggtt tgctttactg gaaaagatgg atgaaatgat gcaccaggaa cagggcacat
      121 tgatgaagag atatcttgcc atgctggata aattatccgt agaagaaaga gaggaatggc
      181 accgggtacg ccacagggtg atgagggttt gtgatgccgc agcccggaat gatgttggtg
      241 tgtctattga tgctgaagaa acatggataa ctgagcccct agatgcattg accattttga
      301 tgatggatac ctttaacaaa gagagggctg tagtatataa taccacacag cattatcgta
      361 cggacaggca gcaattctta aaggattctt acgaggcggc agtagaaaga aattttatcc
      421 tgggtgctaa gctggtaaga ggtgcttaca tggaaaaaga aagaaaaaga gctgctgaaa
      481 gaggttatcc atcacctatt atgccggata aggaaagtac ggacagagac tataatgaaa
      541 gtgtgaaatt ctgtattgac catattgatc gtattgcgtt gatggtagct tcacataatg
      601 aatatagtaa catgtacgct gcacagttat tgcaggaaaa aggcctcccg ttgaatcatc
      661 cgcatgtgca ctggagccag ttatatggca tgagcgacaa tattacattc aacctggctc
      721 atgcaggctg cagcgtaagc aaataccttc cttttggtcc aattaaagat gttataaaat
      781 acctgatgag aagggcacaa gaaaacactt ctgttaaagg gcaaaccggc cgtgagttat
      841 cgttaattaa aaaagaagtt gcccgcagga aaattaaggc ttaaagttcc agcagctgtt
      901 ttaatctaac caggtagctc agctcttcgt ggttgatctt tcgtacactt tcggcaatat
      961 gcctggcgct attgaatatt cttgcacggg tatgttcatc tatctcgttt tttttctccc
     1021 ggaaaaaagt ttcaaatgcc tggtaagcag tttccgctgt ttcgctgtta tcctccatcg
     1081 tttcaaattc aaattcaatc agaaaggccc ggtctgaacc aaaccggtcg gttgtattgt
     1141 cataatgttt ccaggcaaac tgcacttcat catccaatgt tttcttttct ttaggactaa
     1201 tttttttatc agccgcagca atggcataaa ataggttgcc cagttcttta taaaatctcc
     1261 ttgaattaag cataagagct gtattggatt gctacgtcca aattaatgta cgtcatcgat
     1321 tgcgatgctg actaaaatca ctttttttaa tgtttgtcct caattaacag gttgctcaca
     1381 ggcgaagcct ggcagttccg gggtcaaagc tttgctgtaa attgcaggca tgcaacaata
     1441 ccatgatctg ctcagacata ttttagaaaa cggtactgat aaaaaagatc gtaccggaac
     1501 aggtaccagg agtgtttttg gttaccagat gcgttttaac ctgcaaaaag gctttccgtt
     1561 ggttactact aaaaaggtgc acctgaaaag tataatctat gaattacttt ggtttttaaa
     1621 aggagaaacc aatattgctt atctgaaaga acatggagtt tctatctgga atgaatgggc
     1681 ggatgaaaac ggagagctgg gccctgttta tggcaagcag tggcgcagct gggaaggcaa
     1741 agatggagtc gtaattgatc agatcactga tcttattaag caaataaaaa ataatcctga
     1801 cagccgccgg cttatcgtca gtgcatggaa tgtagctgat ctgcccagaa tggcgttgat
     1861 gccctgccat aacatgtttc agttttatgt ggctgaaggc aaactcagtt gccagttata
     1921 ccagcggagt gcagatgtat ttcttggggt accttttaac atagcctcct atgcattatt
     1981 aacgatgatg atagcacagg tttgcgattt agaaccgggt gattttgtgc atacgttcgg
     2041 agatgtacat atttacagta atcacatgga gcaagtaaac ctgcaactta accgaagtcc
     2101 ttttccattg cctgtaatga acctgaatcc ggctgtaaaa gatatcttca gttttaagtt
     2161 tgaggatttt acattggaaa attatcaatg ccatccggct attaaagcac cggtggcagt
     2221 atgatctatt tttaaagtat aatgttcact ttatgatcat ttccctcgtt gttgctgcag
     2281 ccaccaataa tgccattgga aaagatggca aaatgccctg gcatttaccc aatgacatga
     2341 agcattttaa aaatgtaaca tggggtatgc cgatagtaat gggccgaaag acctttgact
     2401 cccttggcaa ggcactgccc ggcagaaaaa atattgtgat aacaagacaa catggctgga
     2461 aggctgataa taccgtagct gtaaaaaata ttgaagatgc cctttttgtt tcaaaagaaa
     2521 cggatgcaaa agaagtgatg gtgatcggag gcggagaaat atacaaagca ctctttgata
     2581 aggcccaaag aatatacctg accagggtag aggcagagcc tgttggtgat actttctttc
     2641 ctgtattgac acaggggcaa tggcaccttg tcagccaaaa gaatcatgaa gcagatgaga
     2701 aaaacgctta taactattcg ttccaggttt gggaaagaat tggataacac tgaatcattt
     2761 tttccatgta ctatgcacca tatttagctc tcaaatatct caggtattat cttacatctt
     2821 ccaatggcaa ggggcatggt acccattcac catttatttt tgatttcatc actaaggtac
     2881 tgaatgataa tagcccttat aaggaataca aacaagcaga agggctgcga aagcggttgc
     2941 tgagtgattc gaggatgttt atcgttgaag attttggtgc aggttctgcc gttttaaagg
     3001 aacaagagcg aagtatcaaa tccatagccc gaaatgcagc caaatccaaa aagtatggtc
     3061 agcttctttt ccggatagtt aaatattacc agcctgccac catacttgaa ctgggcacct
     3121 cattaggaat aacaactact tatttatcgc tggccaatcc tgcttcgaaa attgtgacta
     3181 tggaaggcgc aaaagaaatt gcagccatag caaagcttaa tttccggcaa ttagacataa
     3241 gtaatattga actggttgaa ggcaattttg acaatacttt gcccgacgta tta
//
LOCUS       DAORLS010000327         3272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16007_length_3272_cov_2.013366, whole genome shotgun sequence.
ACCESSION   DAORLS010000327 DAORLS010000000
VERSION     DAORLS010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16007_length_3272_cov_2.013366"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PK977_17525"
     CDS             complement(<1..582)
                     /locus_tag="PK977_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF19983.1"
                     /translation="MKKISFLFIVFLLGTAASFSQADTKMKSFVDALMKKMTLEEKIG
                     QLNLPSSGDITTGQAKSSDIAKKIREGKVGGLFNIKSVAKIKDVQRVAVEESRLKIPL
                     LFGMDVIHGYETVFPIPLGLSCTWDMPLIERSAQIAAKEASADGINWTFSPMVDICRD
                     PRWGRIAEGSGEDAYLGSEIAKAMVKGYQGNDLT"
     gene            complement(593..1261)
                     /locus_tag="PK977_17530"
     CDS             complement(593..1261)
                     /locus_tag="PK977_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRF19984.1"
                     /translation="MIKKYLQKIIIFLVLVSVGLFAKAQPFANEIAAFKKQDSLSFPG
                     TGKILFVGSSSFTLWKDVQQYFPEYPIINRGFGGSSLTDLIRYAPDVIFPYEPKQIVI
                     YCGENDFAGDTSLYPSQVAQRFFDLFNLIRSRYKKVPIAYISMKPSPSRQHLMARFNV
                     ANVMIKNFIKKKRRTAFIDVYKAMLKENGQPKDEIFLADKLHMNAEGYKIWKKIIEPY
                     LLKD"
     gene            complement(1258..>1770)
                     /locus_tag="PK977_17535"
     CDS             complement(1258..>1770)
                     /locus_tag="PK977_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRF19985.1"
                     /translation="DAEFLGKRTDIYHQYKLYYSVDGKKWMVLVDKSTNNKDVPHEYV
                     ELSQPVQARYIKLENIHMPTGKFAISGLRVFGNGNGAMPAPVKTFMVLRTEKDKRSGW
                     IRWSPVDNAYAYNIYYGTAPDKLYNCVMVHDVNEYWFKAMDSQKTYYFTIKAVNENGV
                     SERFKTIEVK"
     assembly_gap    1771..1919
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1920..2802)
                     /locus_tag="PK977_17540"
     CDS             complement(<1920..2802)
                     /locus_tag="PK977_17540"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRF19986.1"
                     /translation="MLNWNFISRKFLRPWNEGYDELCAPAVGIVGDTMIVFGSTYTSK
                     FTLWMSTNPKANEWKPLVDSFEIGGWDPAFFTDDDGRLYMYNGSSNVYPLYGVELDRK
                     TFQPIGTRREMHMLKSWRYGWQRFGEHMDNTFLDPFMEGAHVTKHNGKYYMQFGAPGT
                     EFSGYADGVAVADRPLGNPLGWFQDQSDPLSMKLGGFARGAGHGSTFQDNYKNWWHVS
                     TIMLSVKNNFERRNGIWPAGFDKDDVMWCNTAFGDYPHFLPEQLKNASLKYGDGFSGW
                     MLLNYKKPITVSSTLGNY"
     gene            complement(2796..3050)
                     /locus_tag="PK977_17545"
     CDS             complement(2796..3050)
                     /locus_tag="PK977_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19987.1"
                     /translation="MKFLLLVSFFCCAGVQAQQKTYCNPINIDYGYTPIPNFTEWGKH
                     RATADPVIVNYKNDFYLSLTKAIIIYSVQISGAIGGAAIC"
     gene            complement(3053..>3272)
                     /locus_tag="PK977_17550"
     CDS             complement(3053..>3272)
                     /locus_tag="PK977_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19988.1"
                     /translation="QNSRSISKLVYGPADTWNGFKFNQKPDGCIDYIFVSHYKYLSVS
                     KFATLTDSYDMKYPSDHFPVLATITIDK"
BASE COUNT          883 a          741 c          567 g          932 t
ORIGIN      
        1 tgtcagatca tttccctggt aaccttttac catcgctttt gctatctccg agcccaggta
       61 ggcatcttca ccgctccctt cagcaattcg tccccagcgg ggatcacggc agatatccac
      121 catcggagaa aaagtccagt tgatcccatc ggcgctggct tctttcgctg caatttgtgc
      181 actgcgttca atcagcggca tatcccaggt gcaggaaagt cctaatggta taggaaatac
      241 tgtttcataa ccatgtatca catccattcc aaaaagcaga ggaattttta agcggctttc
      301 ttctaccgct actctttgca catctttgat ctttgccaca gatttgatat tgaacaggcc
      361 ccctactttt ccctccctta tctttttagc aatatcagaa ctctttgcct gtccggtagt
      421 gatgtcaccg gacgatggca ggttgagctg cccgatcttc tcctccaatg tcatcttctt
      481 catcaatgca tcaacaaaag atttcatttt cgtatcagcc tggctgaagg aagctgcagt
      541 gcccaataag aaaacaataa ataaaaaact gatttttttc atctccaatg atttaatctt
      601 tcagtaaata gggttcaatt atttttttcc agatcttata gccttctgca ttcatgtgca
      661 gtttgtcggc aagaaatatt tcatctttgg gctggccatt ttcctttagc atagccttgt
      721 atacatcgat gaatgcagtt ctccttttct tctttataaa attttttatc atcacattag
      781 ccacattgaa cctcgccatc aggtgctggc gagatgggct tggcttcata gaaatatagg
      841 ctatgggtac ttttttatac ctggagcgta tcagattaaa caagtcaaaa aaacgttgcg
      901 ccacttgcga tggatagagg ctggtatcgc cggcaaaatc attctcgccg caataaataa
      961 ctatttgttt gggctcgtac ggaaaaataa catccggggc ataacggatt agatcagtaa
     1021 gggaggagcc gccaaaacca cggttaataa ttggatactc cgggaagtat tgctgtacat
     1081 ctttccataa cgtaaaagaa gaactcccta caaataatat ctttccggtt cccgggaaag
     1141 ataaactgtc ctgtttttta aatgcagcaa tttcattggc aaatggctgg gcttttgcaa
     1201 aaaggccaac cgaaacaagg acaagaaaaa ttattatttt ctgtaaatat ttttttatca
     1261 ttttacttca atggttttaa atcgttcact caccccgttt tcattcaccg ctttgatagt
     1321 gaaataataa gttttctggc tgtccatcgc tttgaaccag tattcattca catcatgtac
     1381 cattacacag ttatataatt tatcaggagc agtgccataa taaatattat atgcataggc
     1441 gttatccact ggactccatc tgatccagcc gctgcgttta tctttttcag tcctcaatac
     1501 catgaaagtt tttaccgggg caggcatagc accgtttcca tttccaaaaa cccggaggcc
     1561 actgatggca aacttacccg taggcatatg tatattttcc agctttatat accgtgcctg
     1621 taccggctgc gatagctcaa catattcatg aggtacatcc ttattattgg tactcttgtc
     1681 aaccagtacc atccattttt ttccatcaac agaataataa agcttatact ggtgataaat
     1741 atctgtccgc ttacccagga actcagcatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1921 gtaatttccg agtgttgacg aaactgtaat cggtttctta taattcagca gcatccagcc
     1981 ggaaaaaccg tcaccgtatt ttaatgatgc atttttcaac tgttccggta aaaaatgcgg
     2041 ataatcgcca aaggcagtat tacaccacat cacatcgtcc ttgtcaaacc cagctggcca
     2101 gataccattt cgcctttcga aattgttttt caccgaaagc atgatggtac ttacatgcca
     2161 ccagttctta taattatcct gaaaggtgga accatgacct gctcctctgg caaagcctcc
     2221 cagtttcata ctcagcggat cactctgatc ctggaaccag cctaacggat tacccaaagg
     2281 cctatctgca acagccacac catcagcata accactgaac tcagtgcctg gcgcaccgaa
     2341 ctgcatataa tattttccat tatgcttggt cacatgtgcc ccctccataa aaggatccaa
     2401 aaatgtattg tccatatgct cgccaaaccg ctgccagcca tagcgccatg atttcagcat
     2461 atgcatttct cttcttgttc ctatgggctg aaaagttttt cggtcgagtt caactccata
     2521 caggggatac acattactgc tgccattgta catatataac cttccgtcat catcagtgaa
     2581 gaaagcgggg tcccatccgc caatctcaaa ggaatcaacc aatggcttcc actcgtttgc
     2641 ctttggattg gtgctcatcc ataatgtgaa tttgctggta taagtagacc caaacactat
     2701 catcgtatca ccgacaatac ccactgcagg tgcacatagt tcatcatacc cttcattcca
     2761 gggccgcaga aattttcttg aaatgaaatt ccagttcagc atatcgctgc tccaccaata
     2821 gccccactga tttgtactga atagataata atcgcctttg taagtgacaa ataaaagtcg
     2881 ttcttataat tgactataac aggatccgcc gtggccctgt gtttgcccca ttcggtaaaa
     2941 ttgggaatgg gtgtataacc ataatcaatg ttgatggggt tacaatacgt tttttgctga
     3001 gcctgtacgc cggcacagca aaaaaaactg actaagagaa gaaacttcat tttcatttat
     3061 caatagttat agtggccaaa acaggaaaat gatctgacgg gtacttcata tcatatgaat
     3121 ccgtcagggt tgcaaacttt gaaacactca gatacttgta atgagaaaca aaaatataat
     3181 caatacatcc atcaggcttt tgattaaatt taaatccgtt ccaggtatca gcagggccat
     3241 aaacaagttt actgatgctg cggctgttct gc
//
LOCUS       DAORLS010000328         3260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16087_length_3260_cov_1.665523, whole genome shotgun sequence.
ACCESSION   DAORLS010000328 DAORLS010000000
VERSION     DAORLS010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16087_length_3260_cov_1.665523"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>269
                     /locus_tag="PK977_17555"
     CDS             <1..>269
                     /locus_tag="PK977_17555"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588332.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HRF19989.1"
                     /translation="LQHLINEALKEDVGDGDHSTLASISPGAKGRAVLKIKQDGILAG
                     LSVAEKIFTYKQPDTKFISFKNDGDEMRYGEKAFEVEALVHTILM"
     assembly_gap    270..398
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <399..850
                     /locus_tag="PK977_17560"
     CDS             <399..850
                     /locus_tag="PK977_17560"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588332.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HRF19990.1"
                     /translation="NFRLLEKEAVKIGGGVNHRFGLYDMIMLKDNHIDYSGGLEKAIE
                     KAARYVKEVKPGLKIEVETRSLDDVKRVIKAGKGIVFRIMLDNFTPEQVHEAVKLIGE
                     EFETEASGGINLDNIEAYAQTGVDFVSVGALIHQARSLDLSLKAVII"
     gene            871..1215
                     /locus_tag="PK977_17565"
     CDS             871..1215
                     /locus_tag="PK977_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRF19991.1"
                     /translation="MSKKNKPDSRGFVYSTDPNFSFEQEQENAETLLPAQQKLRVKLD
                     AKNRGGKTVTLVDGFTGKADDLEDLGKKLKSFCGTGGSAKDGEIIVQGDQRDKVLQWL
                     LKNGYSQTKKSG"
     gene            complement(1446..2783)
                     /locus_tag="PK977_17570"
     CDS             complement(1446..2783)
                     /locus_tag="PK977_17570"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRF19992.1"
                     /translation="MKNNLILLVLLTVFHLQSHAQVSFRNISFDEALKESATNGKLIF
                     IQFESATCTQCNEVADKAFSDAKLGEKMNQSFICLKITNNHTDRQKVASLFNKSNSSF
                     GSLFIAADGSLIHTYARSSTMAKNYEEEAEKALSKAGESVRLKNLEELYKNGNRNLDF
                     LEMYMTAKKNLQLETDKLLDEYVTLIPADSLSSKRMFVFIILMTPLYESVAYQKIEDF
                     YLAVEKRKYNYQYPLKPTVRSSIANKSMRKAIAEKNEPYAHRVADFAGKLYPTDLGKA
                     KKTYESFILDYYKGVNDNTKYLQLAASYYDSYYMTIPVDSVKKADTANMNKLARNQQG
                     TKIKQGDSTVVIRKQIMFASEAGKFSSELTGAAKKFLEITDNADYLSKALKWSEKAIA
                     FNETFTAMNVYALLLYKTNNKPKAISWQEKAIALKKKMGYDTKSLEKELSEMK"
     gene            complement(2804..>3260)
                     /locus_tag="PK977_17575"
     CDS             complement(2804..>3260)
                     /locus_tag="PK977_17575"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRF19993.1"
                     /translation="GQNSIMGKESFKYSNNEITVVWKPNTCIHSTLCWKGLIEVFNPR
                     EKPWIKMVGATTEKIIEQVRKCPSGALSYYRNDELPENPGEVMSESASILKIEVSPNG
                     PYLVKSECLIIHSDGREETKTGTIALCRCGASGNKPYCDGSHRKIDFQE"
BASE COUNT          890 a          553 c          666 g         1022 t
ORIGIN      
        1 ttacaacacc tgatcaacga ggcattgaaa gaagacgtag gagatggcga tcattcaact
       61 ttggccagta tttctccggg agcaaaagga agggcagttt taaagatcaa acaagatggc
      121 atacttgcag gtctatctgt ggctgaaaaa atttttactt ataaacaacc ggatacaaaa
      181 tttatttctt ttaaaaatga tggggatgaa atgaggtatg gggaaaaggc ctttgaagta
      241 gaggcattag ttcatactat tctgatgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aattttcgat tgctggagaa
      421 agaagcggtg aaaatcgggg gaggtgttaa tcaccgcttt ggcttatatg acatgatcat
      481 gttaaaggat aatcatattg attatagcgg cggacttgag aaggctattg aaaaagcagc
      541 aaggtatgtg aaggaggtaa aaccaggttt gaaaatagaa gtagaaacaa gaagcctgga
      601 tgatgtaaaa agagtcataa aagcaggaaa gggaatagta ttccgcatta tgctggataa
      661 ttttactccc gagcaggttc atgaggcagt gaaacttata ggagaggagt tcgaaacaga
      721 agccagcggt ggaattaacc tggacaatat tgaagcgtat gctcaaacag gtgttgattt
      781 tgtaagtgta ggagcattga tacaccaggc acggagcctt gatctgagtt taaaggccgt
      841 gataatttga taatcggata attgaaaata atgagtaaga aaaataaacc tgacagccgg
      901 ggatttgtat acagcacaga ccccaatttt tcctttgagc aggagcagga aaatgcagag
      961 acgcttttac cagctcaaca aaaacttcga gtaaaactgg atgcaaagaa caggggtggc
     1021 aaaacggtta cattagtaga tggttttacc ggaaaggcag acgatcttga agaccttggt
     1081 aaaaaactaa aatcattttg cggcaccggc ggatcagcca aagatggtga gattatcgta
     1141 cagggtgacc aacgggacaa agtgctgcaa tggctgctaa agaatgggta ctcacagaca
     1201 aaaaaatcgg gataggcgat gtggttttat tggttcatta tcttcgggcc tgtgacaatt
     1261 atctacctgg caatagtaat ggtaaactgt ttctatttat ttatgagcct taaacagctc
     1321 ttaaacacag gcttcaaaga tgccatttcc cggaaagcat ttttgtcttc tttgttgtta
     1381 tgctttgctg tgattagtat ttcaagttat gcttacttca aatgggtgtg gttttggtaa
     1441 atgaattatt tcatttcgct caattctttt tcgagagatt ttgtatcata tcccattttc
     1501 tttttaaggg ctatagcctt ttcctgccag ctaattgctt ttggcttatt attagtttta
     1561 tacaatagca gcgcataaac attcatagct gtaaaagttt cattgaaggc gatggccttt
     1621 tccgaccatt ttaatgcttt tgacaggtag tctgcattat cagtgatttc cagaaatttt
     1681 ttggcagctc ccgttagttc gctgctaaac tttccagctt ctgaggcaaa cattatttgc
     1741 tttcttatta ctacagtaga atcgccctgt tttattttgg tgccttgttg atttcgggcc
     1801 agtttgttca tgtttgctgt atcggctttt ttaacagaat caaccgggat agtcatatag
     1861 taggagtcat aatagctggc agcaagctga agatatttag tgttatcgtt tacgcctttg
     1921 taataatcaa gtatgaaact ctcgtacgtt ttttttgctt tgcctaagtc tgtgggatac
     1981 agtttcccgg caaaatccgc tacccggtgt gcatatggtt cattcttttc agcgatggct
     2041 ttacgcatag acttatttgc aatagatgat ctaacagtcg gcttaagcgg gtattgataa
     2101 ttatattttc ttttttcaac agccaggtaa aagtcttcaa ttttttggta cgccacactt
     2161 tcatataacg gggtcattaa gatgataaag acaaacattc ttttcgatga taaagaatct
     2221 gcaggtatta atgttacata ttcatccagt aacttatctg tctctaactg aagatttttt
     2281 tttgcagtca tatacatctc cagaaagtcc aggttacggt tcccgttttt atacaattct
     2341 tccaggtttt ttaatctgac actttcgcct gctttcgaaa gggctttttc agcttcttct
     2401 tcataatttt ttgccatggt tgagcttctg gcatatgtgt gtatcagtga tccatctgcc
     2461 gctataaata gtgaaccgaa actactgttg cttttattaa aaagcgatgc tactttttga
     2521 cggtcagtat gattatttgt aatcttcaga caaataaagg actgattcat cttttcaccc
     2581 agttttgcat ctgaaaacgc cttatctgct acttcattgc attgagtaca tgttgcagat
     2641 tcaaattgga taaaaatcag cttcccatta gttgcagatt ctttgagtgc ttcatcaaaa
     2701 gatatatttc tgaaacttac ctgtgcatgg gattgcaggt ggaatactgt taacagtact
     2761 aaaaggataa ggttattttt catattgatc agctaaagtt agtttattcc tgaaaatcaa
     2821 ttttccggtg gctgccatcg cagtaaggtt tattgccaga ggcgccgcag cggcatagcg
     2881 ctatggtacc tgtttttgtt tcctctcttc catcactgtg aatgatgagg cattccgatt
     2941 ttaccaggta agggccgttg ggtgaaactt caattttcag gatactcgca gattcagaca
     3001 tgacttcgcc gggattttcc ggcaattcat catttcggta atagcttaaa gcaccactcg
     3061 gacattttct tacctgttct ataatttttt ctgttgttgc accaaccatt ttaatccatg
     3121 gcttttccct gggattaaat acttcgatca atcctttcca gcaaagtgtg gagtggatgc
     3181 aggtattggg tttccataca actgtgatct cattgttgga atatttgaag ctttctttac
     3241 ccataatgga attttgtcct
//
LOCUS       DAORLS010000329         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16156_length_3249_cov_1.656230, whole genome shotgun sequence.
ACCESSION   DAORLS010000329 DAORLS010000000
VERSION     DAORLS010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16156_length_3249_cov_1.656230"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..>1277
                     /locus_tag="PK977_17580"
     CDS             351..>1277
                     /locus_tag="PK977_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRF19994.1"
                     /translation="MKTIDSPEDLKKLPREKLHQLCDELRQYIIDVVSVHGGHFGASL
                     GVVELTTALHYVYNTPYDQLVWDVGHQAYGHKILTGRRDNFHTNRKYKGLSGFPKRSE
                     SEYDTFGVGHSSTSISAALGMAMAAKYKGEKDRKVVAVIGDGAMTAGLAFEGMNHAGV
                     ADADMLIILNDNGIGIDPNVGALKEYLTDITTSPTYNKVKDDVWKLLGKLPVGKNFTR
                     SMAHKMAEGLKGMVSSSANLFEALKLRYFGPIDGHNIAKLVDTLKDLREIPGPKILHI
                     ITTKGKGYSLAEQDQTKWHAPGLFDKITGEIKK"
     assembly_gap    1278..1311
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1312..2266
                     /locus_tag="PK977_17585"
     CDS             <1312..2266
                     /locus_tag="PK977_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF19995.1"
                     /translation="YQDVFGHTIIELAELNEKIFGITPAMPSGSSLKFMMEKMPHRAF
                     DVGIAEQHAVTVSAGMATQGMRVFCNVYSSFMQRAYDSVVHDVAIQKLPVVFCLDRAG
                     VVGDDGPTHHGCYDIAYFRCIPNMIISSQMNESELRNLMYTAQLESTKLPFVIRYPRG
                     EGVMPEWKTEMKEIEIGKGRKLKDGKDIAILSFGHPGNFAAAAIRELKNDGLNPAHYD
                     MRFAKPLDEKLLHEACQQYEKLITVEDGTVKGGIGSAILEFMAEHGYKNEVRILGIPD
                     RLVEHGTPKELHRECGYDAQGIADAVRKMMKERITVNQLLG"
     gene            2373..2975
                     /locus_tag="PK977_17590"
     CDS             2373..2975
                     /locus_tag="PK977_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF19996.1"
                     /translation="MKRHTLLLLCIIITCSAIAQLNISIGVKGGLNFANVADASDINN
                     SSKTGYMLGGYIGPKKRTGFGFRSEIILSRQGYNYKTNTNTGNVNLDYLLLPQLFTLN
                     FTKLVQLHAGGQVAILLNANVDSTGGNNNGNLLEYFNRFDYGIVGGAEISPLLGFFIG
                     ARLNISLSAANKNASANWPDFIPKVDTKNNVVQVYTGWRF"
     gene            3068..>3249
                     /locus_tag="PK977_17595"
     CDS             3068..>3249
                     /locus_tag="PK977_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF19997.1"
                     /translation="MKYSRLLLIIFLGCLAVSWFPKTAFWKKTKALNLPKEQKTYLHN
                     PAWMAKLKEKAGEAKS"
BASE COUNT         1018 a          658 c          707 g          832 t
ORIGIN      
        1 ttttttgtgt tgagctgatc aaatgagtaa gcaaagaaaa tattgatcag ggtaaatcca
       61 ccccaaccga tcaattgaaa taaccaatac ctggcagtac caaaccacat agagcaaatc
      121 taatcaatac cttccggcaa acagatacta atataccagt ggcagattaa ttagacaaat
      181 gggcggtatt cttaaaaaaa cctttaaacc ggcattgaaa ggcaaaacct tatcgtaaga
      241 acattgttaa acattgtttt tgccgcaaaa tagctgctgt agctatccgc gggaaaacag
      301 tacttttaca ctttcaagac aaatacatgg aaatcacacc aggaccgctt ttgaaaacca
      361 ttgattcccc ggaggattta aaaaaattac cccgggaaaa attacaccag ttatgtgatg
      421 aacttcgcca gtatataatc gatgtggtaa gtgtgcatgg cgggcatttt ggcgccagtc
      481 ttggggtggt agaactaact actgctcttc attatgttta caacacgcct tatgatcaat
      541 tggtatggga tgtaggtcac caggcttatg gccacaaaat actgacaggc cgccgggata
      601 attttcatac caaccgtaaa tacaaagggc taagtggctt ccccaaaaga agtgaaagtg
      661 aatacgatac tttcggtgta ggccattcct ctacttccat ttctgcagca ttaggtatgg
      721 ccatggctgc caagtataaa ggcgaaaaag acagaaaggt agtagcagtt atcggcgatg
      781 gtgccatgac tgccggatta gcattcgagg ggatgaacca tgctggcgtt gcagatgccg
      841 atatgctcat tattttgaat gacaacggta ttggcattga ccctaatgtg ggcgcattga
      901 aagaatacct tactgatatt accacatctc ctacctataa caaagtaaaa gatgatgtgt
      961 ggaaactgtt gggcaaatta ccagtgggta agaactttac ccgttcaatg gctcacaaaa
     1021 tggctgaagg tcttaaagga atggtgagca gcagcgccaa tttattcgaa gcattgaaac
     1081 tgcgttactt cggtccaatc gatggacata acattgccaa attggttgat acactgaaag
     1141 accttaggga aatacccggt ccaaagatcc ttcatattat tactaccaaa gggaaaggtt
     1201 attcattggc cgagcaagac cagaccaaat ggcatgcacc cggcttattt gataaaataa
     1261 ccggtgaaat aaaaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nataccagga
     1321 tgtatttggt catacgataa ttgaactggc agaactgaat gaaaaaattt ttggtataac
     1381 tccggccatg ccttccggtt catcactcaa attcatgatg gaaaaaatgc cgcaccgtgc
     1441 atttgatgtg ggcatagcag aacaacatgc cgtaacagta agtgctggta tggctaccca
     1501 gggaatgaga gtattttgta acgtctattc ctctttcatg caaagggcat acgattctgt
     1561 agtacatgat gttgctatac aaaagttgcc cgttgtattt tgccttgaca gagctggtgt
     1621 agttggcgat gacggaccaa cccatcatgg ctgctatgac attgcttatt tccgttgtat
     1681 cccaaatatg atcattagtt cgcaaatgaa tgaaagcgaa ctgcgcaacc tgatgtatac
     1741 agcacaattg gaatccacta agcttccttt cgttattcgt tatccaagag gagaaggtgt
     1801 aatgccggaa tggaaaaccg aaatgaaaga aattgagatc ggcaaaggca gaaaattaaa
     1861 agatggaaaa gacattgcca ttctttcctt cggccacccg ggaaactttg cagccgcagc
     1921 tatcagagag ttaaaaaatg acgggctgaa tccggcacat tatgatatga ggtttgccaa
     1981 gccactggat gaaaaactcc ttcacgaagc atgtcaacag tatgaaaaac tgataacggt
     2041 tgaagatggc actgtcaaag gaggaattgg ttctgccatc cttgaattca tggctgaaca
     2101 tggttacaaa aacgaagtaa ggatactggg tatacccgac aggttagttg aacacggcac
     2161 gccaaaagaa ttgcaccggg aatgtgggta tgatgcccag ggaatagccg atgctgtaag
     2221 gaaaatgatg aaagaaagaa ttactgtgaa tcagttactg ggataaattc agaaaacaga
     2281 taaattatca aaccggcaat atgatgttgc cggttttttg tttagtggca gcaaaaatcc
     2341 ttctaccttt agaaggatca aaagccattt gtatgaaaag acacacctta ctattgttgt
     2401 gtattatcat cacctgttca gcaattgcac aactgaatat ttcaattgga gtaaagggag
     2461 gcctgaattt cgccaatgtt gctgacgcat cagatattaa caatagcagc aaaaccggct
     2521 atatgctggg tggatatatt ggccccaaaa aaagaaccgg attcgggttt cgttctgaaa
     2581 ttatcctttc ccgccagggc tataactaca aaacaaacac taatacgggc aatgtaaacc
     2641 tggattacct tttgttgccc cagcttttca cattaaactt cactaaactt gttcagctgc
     2701 atgccggggg acaagttgcg atactgctca acgctaacgt agacagcaca ggcggcaaca
     2761 acaatggcaa cttgctggaa tacttcaacc ggtttgacta tggcatcgtg ggtggcgctg
     2821 aaatatcccc attgcttgga ttttttattg gggcaagact aaatatcagt cttagtgctg
     2881 ccaacaagaa tgcctcagcc aactggcccg actttattcc caaggtggac accaagaaca
     2941 atgttgtgca ggtatatacc ggatggaggt tctgaagttt tcaaaagctt taacgcattt
     3001 tatccaatcc ggttttatgg attttcgtta tcccggtgtc tgactactta ttcaaccgga
     3061 tgcttttatg aaatactccc gactgctgct gatcatcttt cttggctgcc tggccgtatc
     3121 ctggtttccc aaaactgctt tctggaaaaa aacaaaggca ctcaacctgc caaaagaaca
     3181 aaagacgtat ttgcacaacc cggcatggat ggcaaagctg aaggaaaagg ccggcgaagc
     3241 aaaatcatt
//
LOCUS       DAORLS010000330         3220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16369_length_3220_cov_1.517220, whole genome shotgun sequence.
ACCESSION   DAORLS010000330 DAORLS010000000
VERSION     DAORLS010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16369_length_3220_cov_1.517220"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PK977_17600"
     CDS             complement(<1..525)
                     /locus_tag="PK977_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF19998.1"
                     /translation="MIDTTILLRAYRLMCQAKVMAETYEANRSVCKYVHSTSRGHEAI
                     QLATAFQLQPQDFVSPYYRDESLLLGLGFTPYELMLQLLAKGNDVFTGGREYYSHPNY
                     RGEDKPTIIHQSSATGMQAIPTTGVAQGVQYLEKYNNARLKKGFSEELPITICSLGDA
                     SITEGEVSEAFQFAV"
     assembly_gap    616..738
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<739..>1023)
                     /locus_tag="PK977_17605"
     CDS             complement(<739..>1023)
                     /locus_tag="PK977_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRF19999.1"
                     /translation="YDAITTGIDFTARDIQNELKEKGLPWEKAKAWDNSAVIGKWVPI
                     ANIKNKKDINFCLYKNKELVQQGNSSNMIHSFDKIVSYISNYFSVNIGDV"
     assembly_gap    1024..1037
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1038..1271)
                     /locus_tag="PK977_17610"
     CDS             complement(<1038..1271)
                     /locus_tag="PK977_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRF20000.1"
                     /translation="MKIFCVGRNYVAHAAELGNEVPDEPVIFMKPKSAMLQPHTPFYY
                     PEFTNELHYECELVLRISKNGKYIQEKFASKYYD"
     assembly_gap    1393..1490
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1491..>1760)
                     /locus_tag="PK977_17615"
     CDS             complement(<1491..>1760)
                     /locus_tag="PK977_17615"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRF20001.1"
                     /translation="IVDDIALPLEKLRIRPSGSDAGHNGLKSIQEVLATTEYPKLRFG
                     IGNNYPKGHQADFVLSKWNKDEEPLVKLKIGKAVEAIEIFATQGIT"
     assembly_gap    1761..1840
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1841..2038)
                     /locus_tag="PK977_17620"
     CDS             complement(<1841..2038)
                     /locus_tag="PK977_17620"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRF20002.1"
                     /translation="MKFLIVGLGNIGNEYAHTRHNIGFDVVNAFVLKHSGAFVTDRLA
                     YFSQVKWKGKNFICICPTTYMN"
     gene            complement(2120..2752)
                     /locus_tag="PK977_17625"
     CDS             complement(2120..2752)
                     /locus_tag="PK977_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRF20003.1"
                     /translation="MKTITIEGQLRTGFGKGATRQLRSQELVPGVIYGGSQEINFQAP
                     AAAFKNLVYTPSFQIAEVVLDGKTYRTILKDLQFDKVNDSLIHVDLLELVEDKKVIAD
                     IPLKFVGASKGVKDGGKLVTKMKSLKVKTYPKYLKTQIEVPIDDLELNGNIRVEDVKE
                     ENYEILNSPRIPIASVVLTRQLKQEEAAAPAAGAAPAAAAAPAAAPAAKK"
     gene            complement(2773..>3220)
                     /gene="prs"
                     /locus_tag="PK977_17630"
     CDS             complement(2773..>3220)
                     /gene="prs"
                     /locus_tag="PK977_17630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRF20004.1"
                     /translation="DVGATNRIREIASYFNAEMVICDKHRKRANEIASMVVIGDVTDR
                     DVVLIDDICDTGGTLAKSAGLLKEKGARSVRALITHPVLSGKAYENIENSVLEELVVC
                     DTIPLKKESSKIKVVSVSELFAVAIRNAYENKSITSLFIHSQRKDK"
BASE COUNT          781 a          651 c          567 g          906 t
ORIGIN      
        1 tacagcaaac tgaaatgcct cactcacttc tccttctgta atactggcat ctcccagtga
       61 acatatagtt atgggcaact cttcgctaaa tcctttttta agtcttgcat tattatattt
      121 ctccaaatac tgaactcctt gcgccacacc tgtggtcggt atggcctgca tacctgtagc
      181 actgctctgg tgtataattg tgggcttatc ttctcctcgg tagttaggat gagaatagta
      241 ttcacggcca ccggtaaaga catcattgcc tttggccagc aattgaagca taagttcata
      301 aggagtgaat cctaatccca gcaacaaact ttcatcccgg taataaggac ttacgaaatc
      361 ctgtggctgc aattgaaaag cagttgccag ttgaatagct tcatggccac gggaagtaga
      421 atgtacatac ttgcaaacgg agcggttggc ttcataggtt tctgccatca cttttgcctg
      481 gcacatcagc cggtaagccc ttaataatat agttgtatca atcatgtaat agcaagcagg
      541 taagaggcaa aattaaggga aaggccatgg ctgcaaaagc aaaaaccgct ctatattcag
      601 agcggttttc agcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnat tacatcaccg atatttactg agaagtaatt agaaatataa
      781 gacacaatct tgtcaaagct gtgtatcata tttgaagaat ttccctgttg taccaattct
      841 ttgttcttgt acaagcagaa attaatatcc tttttgtttt tgatattggc aataggcacc
      901 catttgccaa ttacagctga attgtcccag gctttggctt tttcccaggg caaccctttt
      961 tccttcagct cattttggat atctcttgct gtaaagtcta ttcctgtggt gatagcatca
     1021 tagnnnnnnn nnnnnnnatc gtaatattta ctggcaaatt tctcttgtat atattttccg
     1081 ttcttgctta tacgtaatac aagttcacat tcataatgga gttcgttggt aaactcagga
     1141 taataaaacg gggtatgtgg ctgcagcatg gcacttttag gcttcatgaa aattaccggt
     1201 tcatccggta cctcattacc caactcagca gcatgtgcta catagtttcg accaacacaa
     1261 aaaatcttca taaacaggca gagtttaaat taaatacacc ggataggagc actcaaactg
     1321 gtggttatcc catgtatcaa ttggtaatca aaaagtctat tggattctaa aacccgggcc
     1381 gtgaaaaaat ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtaataccc
     1501 tgtgtggcaa atatttcaat ggcttcaacg gcttttccga tcttcagttt caccaggggc
     1561 tcctcatctt tattccattt actcaaaaca aaatcagcct ggtggccttt tggataatta
     1621 ttgccaatac caaatctcag tttgggatac tcggtagtgg ccaaaacctc ctggatgctt
     1681 tttaaaccat tatggccagc atcgcttcct gatggacgga ttctgagttt ttccagggga
     1741 agggcgatat catccacaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttcatgtaa gttgtggggc
     1861 agatacatat aaaatttttg cccttccatt ttacctggga aaaataagcc agcctgtcag
     1921 ttacaaatgc accactatgt ttaagtacaa atgcattcac cacatcaaag ccgatattat
     1981 gcctggtatg tgcatattca ttgccaatat tccctaaacc aacgatcaga aacttcataa
     2041 ctgaattcaa atatcggatt aagaaaagaa aaacccgtca ggcaaagcga gacgggtttt
     2101 attatttttt tgaaagatat tacttcttcg cagcaggagc agcagcgggt gcagcagcag
     2161 cggcaggcgc agcgccggca gcaggagcag cagcttcttc ctgtttcaac tgtcttgtca
     2221 gtactactga agcgatagga atacggggag agttgagtat ctcataattc tcttccttta
     2281 catcttctac cctgatattt ccgttcagtt ccaggtcatc gattggaact tcgatttgtg
     2341 ttttcaggta ctttggatag gttttcactt tcagtgactt catcttggta accagcttac
     2401 cgccatcctt tacacccttt gatgctccaa cgaacttcag gggaatgtca gcgattactt
     2461 ttttatcctc aaccagctcc agcaggtcta cgtggataag agagtcattt actttgtcaa
     2521 actgcaggtc tttcagaatg gttctgtacg ttttaccatc taatactact tcagcgatct
     2581 ggaaacttgg tgtgtacact aaatttttga aagccgcagc aggagcctgg aaattgatct
     2641 cctgagagcc gccataaatt acaccaggca ccaattcctg agagcggagt tgtcgggtgg
     2701 cgcctttgcc gaatccggtc ctcagttgtc cttcgattgt aattgttttc atttgttatg
     2761 ttgttttaaa atttacttgt cttttcgctg cgaatgaata aagagactgg tgatactctt
     2821 gttttcatat gcatttcgta tagctaccgc aaaaagttca gacacactta ctactttgat
     2881 ctttgaactt tcttttttaa gtggtatggt atcacaaacc accagttctt ccagtacact
     2941 gttttcaata ttctcatatg ctttgccgct cagtaccggg tgggtgatca atgcccttac
     3001 gcttcttgct cctttttctt ttaaaagacc tgcacttttt gccagcgtac cgccagtatc
     3061 acatatgtca tcaatcaaaa ccacatccct gtcggtcaca tcacctatca ctaccatgct
     3121 ggcgatctcg ttggcccttt tacggtgctt atcacagata accatttctg cattaaagta
     3181 gctggcaatc tccctgatgc ggttggttgc tcctacgtca
//
LOCUS       DAORLS010000331         3210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16449_length_3210_cov_1.802853, whole genome shotgun sequence.
ACCESSION   DAORLS010000331 DAORLS010000000
VERSION     DAORLS010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16449_length_3210_cov_1.802853"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>742)
                     /locus_tag="PK977_17635"
     CDS             complement(<1..>742)
                     /locus_tag="PK977_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20005.1"
                     /translation="LFSDNLKAASGLGMIAIDVVHTAHGLAGAEVFIGEWGKAFAEVD
                     MLRRGKWKLLEVERNENGQIIDIKTVEKEGKQLFMEKGMELELKAQQKDPANSFDPKE
                     RYPELRIDMEPMLSLEMLRHAIHDIEHGPFQGGQKIIKMIKYTERSFFMIKEALKSVS
                     VKDQMLYFNLTLDEQRLMTLTEEVIKNKGDAKKIAGLLEEFTGNKLESNEQVNLIVDD
                     IVQKCKKAMLKNANQAFGYRVRTIAAIEN"
     assembly_gap    743..880
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<881..1445)
                     /locus_tag="PK977_17640"
     CDS             complement(<881..1445)
                     /locus_tag="PK977_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20006.1"
                     /translation="MLPLPSMRQGFIAILAGLLFASITATAQTTEDLEKMARDAKPFQ
                     FHLKFRGSVVDPLEVKYAKITALLDKYNKGVPGHLQQPFKVQGPEASQKPNLSIEQEA
                     SLKNYSTQFFQEMAKLKLTVWDLQEYKDKFKNKNPMPGDSPVDPKIAWKGEFEGIERT
                     LMGGRTLMIMDVWKQTMIEFFRNHPEAV"
     gene            complement(1587..2681)
                     /locus_tag="PK977_17645"
     CDS             complement(1587..2681)
                     /locus_tag="PK977_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRF20007.1"
                     /translation="MPDLRSIQTEVDASYLYSKLAGKEEDKNVADIFQQMSDIEKGHA
                     EAFLKKIGLTPDKMPPPSFRAKTLNRIGNIFGYDYVLGVLMDTEKSLSHSVLAAKKKT
                     NQPVTGSEVNHVKVLRNIIEQSKVSGSTIARFESRHKSVGGNALRAAVLGGNDGLVSN
                     FSLVMGVAGATQGGSGVLLAGLAGLLAGALSMALGEWISVKSSQELFENQMAIEMEEL
                     ETSPENEMKELELIYKAKGIPEAQAKEMAAEIIRNPEHAHELLVKEELGINAEELKGS
                     AFEAAAYSFILFGIGAIIPVFPFFFLSGTNAILLSAAASAVGLFIIGAAITLFTGKSI
                     WYSGFRQVLFGLAAAAITFGIGKLIGVSVT"
     assembly_gap    2767..2804
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2805..>3210)
                     /locus_tag="PK977_17650"
     CDS             complement(<2805..>3210)
                     /locus_tag="PK977_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20008.1"
                     /translation="AALFTAPVFLTGEGTEELVEKLPGVNEGAIEEHEEMAKISMIII
                     AATGVVALIGLLARKNKSIGKFVFLAAAILSLASFGTMAQTAHLGGLIRHTELQNGAA
                     TSEKENEEKEDDNNKAQQKEVKDSLKQAERTIK"
BASE COUNT          763 a          695 c          564 g         1012 t
ORIGIN      
        1 cattttctat tgctgcaatg gttctaaccc ggtatccgaa agcttggtta gcatttttca
       61 acatggcctt tttacacttt tgtacaatgt catctacaat caggttcacc tgttcattgc
      121 tctccagttt attaccagta aattcttcta ataggccggc tatctttttt gcatctcctt
      181 tgtttttgat gacttcttct gtcaacgtca tcagcctttg ttcatctaat gtcaggttga
      241 aataaagcat ctggtctttt acggatacac ttttcagcgc ttctttgatc atgaagaaac
      301 ttctttcagt gtatttgatc attttaatga tcttctgtcc cccctggaat ggaccatgtt
      361 caatatcatg tatggcatgc cggagcattt cgaggcttag cataggttcc atatctatgc
      421 gcagctccgg gtatctttct ttggggtcaa aagaattggc aggatctttt tgctgggctt
      481 tcagttccag ttccatacct ttttccatga agagttgttt cccttccttt tcaactgttt
      541 taatatctat aatctgaccg ttttcatttc tttctacttc aagcagtttc catttaccac
      601 gcctcagcat atctacttct gcaaatgctt ttccccattc ccctataaat acttcagcac
      661 cggcaaggcc atgtgcggta tgtactacat caatggcaat catacccagg ccgctggcag
      721 cttttaaatt atcactaaaa agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccactgcttc aggatgattc
      901 ctgaaaaatt caatcatggt ttgtttccat acatccatga tcatcagggt tcggcctccc
      961 atcaatgttc tttctattcc ttcaaactcg cctttccagg caatctttgg gtctaccggg
     1021 ctatcacccg gcattgggtt tttgtttttg aacttgtctt tatattcctg gagatcccaa
     1081 acggtaagtt tcagtttggc catttcctgg aaaaactgag ttgaataatt ctttaaggaa
     1141 gcttcctgct caattgacaa attcggtttc tggctggcct cgggtccctg tactttaaat
     1201 ggttgttgaa ggtgacctgg gacaccctta ttatatttat ccaataaagc agtaattttt
     1261 gcgtatttaa cttcaagagg atcaaccaca gatcctctga atttgagatg gaattgaaat
     1321 ggcttggcat ccctggccat tttttcaaga tcttctgttg tttgagctgt agcagtaatg
     1381 ctggcgaata ataatcctgc aaggatggcg ataaacccct gcctcatgga tggaagaggt
     1441 aacatatata aagagtttag gtaagtaggc ttttgcaagc tactgataga cattccctaa
     1501 aaaaatgatt gctgtcacag attggagtga cagcaatcaa aacaattttt atttcaaaaa
     1561 gacttgtttg tctcttttat tatgctctat gtaacactta cccctatcag ttttccgatt
     1621 ccaaatgtaa tagcagcagc ggctaatcca aatagtacct gcctgaagcc ggagtaccaa
     1681 atactttttc ctgtaaatag ggtaatggct gctccaataa tgaaaagacc aactgcactg
     1741 gcagcagcac ttaaaaggat agcgttggta ccacttaaga aaaagaaggg gaagacaggg
     1801 ataattgcac caataccaaa taagatgaaa gagtaagctg cggcttcaaa ggcagatcct
     1861 tttaactctt ctgcattgat accgagttct tcttttacca acagttcatg ggcatgttcg
     1921 ggattcctga ttatttcagc cgccatttct ttggcttgtg cttccgggat acctttagct
     1981 ttatagatca gttccagctc tttcatttca ttttcagggc tggtttccag ttcttccatt
     2041 tcgatggcca tctggttttc aaataactcc tgtgagcttt ttacagatat ccattcaccc
     2101 aatgccatag ataatgcacc ggccagcaaa ccggctaacc cggctaataa aacaccactg
     2161 ccgccttgcg tagcaccggc cacacccatc accaggctga agttggaaac aagaccgtca
     2221 ttaccaccaa gtactgcagc ccgcagggca tttccaccaa ctgatttatg ccttgattca
     2281 aaccgggcta ttgtactgcc tgataccttt gattgctcaa tgatattgcg aagcactttt
     2341 acatgattta cttcggaacc ggttaccggc tggttggttt tcttttttgc tgccaataca
     2401 gaatgggaaa gacttttttc tgtatccatt aatacgccca gcacataatc ataaccaaaa
     2461 atatttccga tccggtttag tgttttggcc cgaaaggagg gtggaggcat tttgtctggg
     2521 gtaagcccga tttttttcag gaatgcttct gcatgccctt tttcaatatc actcatctgc
     2581 tgaaaaatat cagccacatt tttatcttct tcctttccgg ctaatttgct gtagagataa
     2641 ctggcatcaa cttctgtttg aatacttctg agatcgggca taactagatt tttctgatta
     2701 aagatactta ctgctcaacc agtatttact gtctgcgatt gcttcatcca atgattttta
     2761 ttaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttaa ttgtcctttc
     2821 cgcctgttta aggctgtcct ttacttcttt ttgctgcgct ttattgttgt catcttcctt
     2881 ttcttcgttt tctttttcac tcgttgcagc gccattttgc aactctgtgt gacggatcag
     2941 tccaccgaga tgagcagttt gtgccatagt gccaaatgaa gctaaggaaa gaatggctgc
     3001 tgcaaggaag acaaactttc caatggattt gtttttcctt gccaataaac caataagtgc
     3061 aacaacacca gttgcagcaa tgatgatcat ggatattttg gccatttctt catgctcttc
     3121 gatagccccc tcatttacgc ctggcagttt ctcaactaat tcttctgttc cttccccggt
     3181 caaaaaaacc ggggctgtaa acaacgctgc
//
LOCUS       DAORLS010000332         3182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16645_length_3182_cov_1.854813, whole genome shotgun sequence.
ACCESSION   DAORLS010000332 DAORLS010000000
VERSION     DAORLS010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16645_length_3182_cov_1.854813"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>237)
                     /locus_tag="PK977_17655"
     CDS             complement(<1..>237)
                     /locus_tag="PK977_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20009.1"
                     /translation="IHTVIPANAISGNNTILPKDSSAKTYKSIREFSASIKDKEGKLL
                     KWKERKKLLKEQVRSIKKADNLSKGDKAGLIVLAV"
     assembly_gap    238..380
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<381..560)
                     /locus_tag="PK977_17660"
     CDS             complement(<381..560)
                     /locus_tag="PK977_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20010.1"
                     /translation="MRKLSFWARYHKKAARLIIFSSFLLLTIIGYYTGTWMNDLGISL
                     SETILFLAVFLYIAGV"
     gene            complement(581..1021)
                     /locus_tag="PK977_17665"
     CDS             complement(581..1021)
                     /locus_tag="PK977_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20011.1"
                     /translation="MKAIVTITCLWLLTQTVVAQSTDQPVLSKADYLKKGKNQSTAAW
                     ILVGGGAAMVSIGSIIGLTDATEAIGSIFTGETEEPSNAGPILFFTGTAAMLGSIPLF
                     IASSRNKRKANSMSAFFKMENRPLLHRSSFTKASYPAMGFKIKL"
     gene            complement(1132..1716)
                     /locus_tag="PK977_17670"
     CDS             complement(1132..1716)
                     /locus_tag="PK977_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF20012.1"
                     /translation="MIAQLLERLNSLNPMSEELADKIISVTKTIDYPAGTYILKEGQT
                     CTRACMIGKGLARSYYINDDKEITSRFMDEGFIITSWISYYTQKPGNEFIQAVEDCTL
                     LCIDHADIQQVYKEFPEFNIIGRKQTEYAFYQAELRTQMLRKHTAEERYKFFIEQQPS
                     LLQRVSLKHIATYLGMNEETLSRVRSRFHKKHSL"
     gene            1841..2542
                     /locus_tag="PK977_17675"
     CDS             1841..2542
                     /locus_tag="PK977_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRF20013.1"
                     /translation="MKKLITILLLSVALSSCEILTNLPTGTGGGVTETEAADGIKEAL
                     GQGLVKAVLQLNKEDGFFKDALYKVLLPPDAKKIENTLRDLGMSKMVDKAILQINRAA
                     EDAAGYAKPIFVDAIKSMTIADAIGLVRNGDTSATHFFRVKTTDKLIAAFSPVIKSSL
                     DKLDATKYYADVVNTYNNFPTTFKKINPDLPSHVTGKAVDALFDLVGKEEQNIRQNIA
                     ARTTDLLRKVFGAKW"
     gene            2647..>3182
                     /locus_tag="PK977_17680"
     CDS             2647..>3182
                     /locus_tag="PK977_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRF20014.1"
                     /translation="MKTLFKVLFFAVVIIPSLSSAQIKLPGILSNKSSGITENEAGQG
                     IKEALLQGVTNAVLNLNKTDGFFGSEIYKMLLPPDAVKVEKTLRNLGMGNQVDKAILA
                     INRGAEDAVAYAKPIFIDAIKEMTLKDALNIVKGPKDAATSYFREKTKQKLITAFSPS
                     VKSSLDKKEDTKYYNDIIT"
BASE COUNT          896 a          593 c          621 g          929 t
ORIGIN      
        1 acagccaata caataaggcc tgccttatct ccctttgaca ggttgtcggc ctttttaatg
       61 ctgcgaacct gttctttcag tagctttttc ctttccttcc attttagcag ctttccttct
      121 ttatccttaa tagaggctga aaattcccgt attgatttat aggttttggc cgagctgtct
      181 ttgggtaaaa ttgtattgtt tccggagatg gcattggccg gaataaccgt atgaatcnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn tactccggcg atatacaaaa acacagctag aaatagaata
      421 gtttccgaaa gcgatatacc caggtcattc atccaggttc cggtataata accgataatg
      481 gtaagtaaca aaaaggaaga aaaaattata agacgggctg cttttttatg gtacctggcc
      541 caaaatgata attttctcat atagcagggt ttaggagtga ttaaagcttt attttaaatc
      601 ccatggctgg ataagatgct tttgtaaaag aacttctatg aagtaaaggc cggttttcca
      661 ttttaaagaa agcggacata ctattggctt ttcttttatt tcttgaagag gcaataaata
      721 aaggaatgct gcccagcatg gcagctgttc ctgtgaagaa aaggatcggt cctgcattag
      781 atggctcttc tgtctctccg gtgaaaattg agccaatagc ttcagtagca tcagtcagcc
      841 caataattga tcctatggat accattgctg cgccgccacc cactaatatc catgcagcag
      901 tactttgatt tttacccttc ttcaaataat ctgctttgga taataccggt tgatccgtag
      961 attgggcaac cacagtttgt gtcagcaacc ataagcaggt aatggtaaca attgctttca
     1021 tataatcgtt ttgatgaagt tgagaaataa aactgttttt ttgcttgaca tatgtcaaga
     1081 gttggagttg agtaatgatt attgtcaaat cagttttttc cctggctttt gttaaagact
     1141 gtgcttctta tgaaaacgac ttctaaccct gcttaatgtt tcttcgttca tcccgaggta
     1201 agtagcaata tgtttcaggg atacccgctg cagcagtgaa ggttgttgct ctataaaaaa
     1261 tttatatctc tcttcggctg tatgcttgcg aagcatttgg gtccttaatt ctgcctggta
     1321 aaaagcatat tctgtttgtt tcctgccgat gatgttgaat tcgggaaatt ctttgtatac
     1381 ctgctgtatg tctgcatggt caatgcataa gagtgtgcaa tcttctaccg cttgtataaa
     1441 ctcgttaccg ggtttttgtg tataatagct gatccacgaa gtgatgataa aaccttcatc
     1501 catgaacctg gaggttattt ctttgtcatc attgatgtaa taagatcttg ccagtccttt
     1561 acctatcata caagccctgg tgcaggtttg tccttctttc aaaatataag tgccggcagg
     1621 atagtcgata gtcttcgtaa cagatattat tttatctgcc agttcctcac tcatggggtt
     1681 aagtgagttt agcctctcaa gaagctgggc tatcatttac ctaatttacg ggtaataatc
     1741 agcaatcccg gattttatat tcagtacctt taaatactta aattttttaa aaaatattca
     1801 ttgcatacaa actgaccact gaatgataaa aaccttcaac atgaaaaagc taattacgat
     1861 attactgttg tcagttgccc tgagcagttg tgaaatactg actaatctcc caacaggcac
     1921 tggcggcggt gttacagaaa ctgaagcagc tgatggtata aaagaagctt tgggccaggg
     1981 cctggtgaaa gctgtcctgc aactcaataa agaagacggt ttttttaaag atgcattgta
     2041 caaggttctg cttcccccgg atgcaaagaa aatcgagaac acccttcgtg accttggcat
     2101 gagtaaaatg gtggacaaag ccatcctgca gatcaaccgg gcggcagaag atgcagcagg
     2161 atatgccaaa ccgatctttg ttgatgccat taaaagcatg acgattgctg atgccatagg
     2221 cctggtaaga aatggagata ccagcgccac tcattttttc cgggtaaaaa caaccgataa
     2281 actcattgct gccttttcac cagttatcaa aagctcattg gataaactgg atgccacgaa
     2341 gtattatgcg gatgtagtga atacgtataa taattttcct actacattta aaaaaatcaa
     2401 tcccgattta cccagccatg ttacgggtaa agctgttgat gctttgtttg acctggtggg
     2461 aaaagaagaa caaaatatcc gccagaatat tgctgcccgc actactgatc tgctccgaaa
     2521 agtgtttggt gcaaaatggt aaagaccgga gcgcaacgaa ttgcttaaaa tatcgtcaaa
     2581 ataaagcggt aaggtttttg tattaactga accgctttat tttacatttt aaaaggtatc
     2641 atacttatga aaacactatt taaagtactt ttctttgctg ttgttatcat tccttccctg
     2701 tcttctgcac aaatcaaact acccggcatc ttaagcaata aatcttccgg catcacagaa
     2761 aatgaggcgg gccagggaat taaagaggct ttgctgcaag gcgtgacaaa tgctgtactt
     2821 aatctgaata aaactgatgg ttttttcggt agtgagatat ataagatgtt gctgccgccc
     2881 gatgctgtga aggtggagaa gaccttgcgt aacctgggaa tgggtaacca ggtagataaa
     2941 gccattcttg ccatcaaccg tggtgctgaa gatgcggtag cttatgccaa acccatcttt
     3001 atagatgcca tcaaagaaat gacattgaaa gatgcgctga atattgtgaa aggtcctaaa
     3061 gatgctgcca ccagttattt cagggaaaaa acaaaacaaa agctgatcac cgctttttct
     3121 ccttcggtca aatcttcttt ggataaaaaa gaggatacga aatattataa tgatatcatt
     3181 ac
//
LOCUS       DAORLS010000333         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16661_length_3179_cov_1.882522, whole genome shotgun sequence.
ACCESSION   DAORLS010000333 DAORLS010000000
VERSION     DAORLS010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16661_length_3179_cov_1.882522"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK977_17685"
     CDS             <1..304
                     /locus_tag="PK977_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRF20015.1"
                     /translation="PIVGLVENMSYFVPAELPDNKYYIFGKEGGKRLADEYDLPFLGQ
                     IPLVQSIREGGDQGVPVMVSDDMVSRKAFEGFASYVVRSIAMRNANIASEKIAETV"
     gene            405..1043
                     /locus_tag="PK977_17690"
     CDS             405..1043
                     /locus_tag="PK977_17690"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRF20016.1"
                     /translation="MYNYHHYKEHDVAEVLRFMRQHPFVLLVAADQSGRVEATQVPVI
                     IDEKDGKIFITGHIARKSDHHKALEENPDALVIFTGPHVYVSGTWYTGNPQQASTWNY
                     IAVHARGKVRWAKEEELIGILRRLSLHFENGNTASSTIYDNLPEEYLSKLVKAIVGFE
                     IEVTELDNVHKLSQNRDEKSYDNIVKELKQQEGDGKIIGEIMENRKSKVFPS"
     gene            1040..1960
                     /locus_tag="PK977_17695"
     CDS             1040..1960
                     /locus_tag="PK977_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRF20017.1"
                     /translation="MRNNILPVLLVFFLACSTTKKVPEETSVTTNEKYVQGEFDKQGH
                     RGCRGLMPENTIQAMIYALGTPATTLEMDVVITKDKKVVLSHEPFFNHEITTKPDGKY
                     VTEAEEKSLNIYQMTYEDVKKYDVGMKPHPRFPKQQKLRTVKPLLADLVDSVYNYMMT
                     ARRPFPCYNIETKCLPAGDGIYHPSPAEYVELLVAVIKEKNIEDHVIIQSFDFRTLQY
                     INQKYPHIKTAMLIEDDDKRGLDEQLKALGFTPTIYSPHFSLVNTDLLKKCHDKNIKV
                     IPWTVNDKQQIDDLKKMGVDGVITDYPDLF"
     gene            complement(1967..>2810)
                     /locus_tag="PK977_17700"
     CDS             complement(1967..>2810)
                     /locus_tag="PK977_17700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF20018.1"
                     /translation="IRRKDMEEADVDIQVLSTIPVLFNYWAKPLDGLETSRFFNDHIA
                     ETAAKEPNHFIGLGTVPLQVIDLAIKEMERCVKELKMPGLEIGSNINGKNLSDKEFFP
                     FYEAAQKLDCALFIHPWEMMGEDNMQKYWLPWLVGMPAETSRAICSMIFGGVFEQFPK
                     LRVAFAHGGGSFPFTIGRIEHGFKVRPDLTAVDNPVNPRDYIGKFWIDSLVHDKKAFE
                     YLLEVMGEDNICLGSDYPFPLGEHKPGRLIQKMDLGKKITNKLLYKNAVKWLGIDKKS
                     LPEF"
     assembly_gap    2811..2870
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          947 a          595 c          685 g          892 t
ORIGIN      
        1 gcccatcgtc ggactggtgg agaatatgag ttatttcgtt cccgcagaat tgccggataa
       61 taaatattac atatttggca aggaaggtgg taagcggctg gccgatgagt atgatcttcc
      121 tttcctggga caaattccgt tggtgcaaag tatccgtgag ggaggtgacc agggagtacc
      181 ggtgatggtg agtgatgata tggtgagccg taaagccttt gaaggatttg cttcttacgt
      241 ggtaagaagt attgccatgc gaaatgccaa tattgcttcg gaaaaaattg ctgagaccgt
      301 ttaatggtaa gagtttgagt ttaatgttct tttgaatgga agcagtattg ttaatttgat
      361 ttgctttagg tactaagact ttaactttaa actttaaact atccatgtac aactatcacc
      421 actacaaaga acatgatgtg gcagaagtat tgcgcttcat gcggcagcat ccgtttgtgt
      481 tgctggttgc agctgatcaa agtggaaggg tagaagcaac ccaggttcct gtaattattg
      541 atgaaaagga tgggaagatt tttattaccg gacatattgc cagaaaatca gaccatcata
      601 aagcattgga agaaaaccct gatgccctgg tgatttttac aggccctcat gtttacgtta
      661 gtggtacatg gtacactggt aacccgcaac aggcttctac ctggaattac atagccgtac
      721 atgcaagagg gaaggtaaga tgggcaaagg aagaagaact gatagggata ctccggagac
      781 tttctcttca ttttgaaaac gggaatacgg catcgtcaac aatttatgat aaccttcccg
      841 aagaatacct ctccaagctg gtgaaggcga ttgtaggttt tgaaatagaa gtaacagaat
      901 tggataatgt tcataagctg agccagaacc gggatgaaaa gagctatgat aatattgtga
      961 aagaactaaa gcaacaagaa ggtgatggaa agataatcgg agagataatg gaaaacagaa
     1021 aatcaaaagt gtttccgtca tgagaaataa tattttacca gtgttgctgg tatttttttt
     1081 agcatgctca actactaaaa aggtgcctga agaaacttct gtaacaacaa atgagaaata
     1141 tgtacagggt gaatttgata aacagggtca ccgtggttgc cgtggtctga tgccggagaa
     1201 tactattcag gccatgattt atgcgttggg tacacctgcc actacgctgg aaatggatgt
     1261 agtgatcaca aaagataaaa aagttgtttt atcacatgaa ccatttttta atcatgaaat
     1321 aactacaaag ccggatggta agtatgtaac agaggcagaa gaaaaaagcc tgaatattta
     1381 ccagatgaca tacgaggatg taaaaaagta tgatgtgggc atgaagccgc atccgcggtt
     1441 cccaaaacag cagaagttaa ggactgtaaa accattactg gcagaccttg tcgacagtgt
     1501 gtacaattat atgatgacgg ccaggcgtcc ttttccatgt tataatattg aaaccaaatg
     1561 cctgcctgcc ggcgatggta tttatcatcc ctcgcctgct gaatatgtag aattactggt
     1621 ggctgttata aaagaaaaga atatcgagga tcatgttatc atccagtctt ttgatttcag
     1681 gacgctacag tacatcaacc aaaaatatcc gcatattaaa acagctatgc tgatcgaaga
     1741 tgatgataaa aggggattgg atgaacaatt gaaagcttta ggctttacgc cgactatcta
     1801 tagtccgcat ttttcgttag tcaatactga tttgttaaag aaatgccacg acaagaacat
     1861 taaggtgatt ccctggactg ttaacgataa gcaacaaata gatgacttga agaagatggg
     1921 agtagacggg gttattaccg attacccgga tcttttttaa gatggattaa aactctggca
     1981 aacttttctt atcaataccc agccacttca cagcgttttt gtaaagcagt ttattggtga
     2041 tctttttacc caaatccatt ttctggatca ggcgtccggg cttatgttcg cccagcggaa
     2101 agggataatc actgccaaga catatattgt cctctcccat tacttccagc aagtattcaa
     2161 aagctttctt gtcatgaact aaactgtcaa tccagaattt accaatataa tctcttggat
     2221 taacaggatt atcaacagca gtcagatcgg gacgcacctt gaatccatgc tcaattctgc
     2281 caatagtgaa aggaaaggag ccgccaccat gtgcaaatgc aactcttaac ttcgggaatt
     2341 gttcaaatac accgccaaag atcatagaac agatagccct ggaagtttcg gccggcatac
     2401 ctaccagcca gggcagccag tatttttgca tattgtcttc tcccatcatt tcccagggat
     2461 gaataaataa agcacaatcc agtttttgtg ccgcttcata aaaaggaaag aattctttat
     2521 cggataaatt tttcccattg atattggagc caatttccag tcccggcatt ttcagttctt
     2581 tcacacaacg ttccatttct ttaatggcaa gatcaataac ctgcaatggc actgttccca
     2641 gtccgataaa gtgattgggt tctttcgctg ccgtttcagc aatatggtca ttaaaaaaac
     2701 gggaggtttc cagcccgtct aaaggtttag cccaataatt aaagagaacg gggatagtgg
     2761 acagaacctg tatgtccaca tctgcttctt ccatgtcttt tctgcgaata nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccttcatcat
     2881 acaggcatcg cagttgcggt gttccaggtg aatgaattca ccatagccaa acttctgtgt
     2941 ccagtttggc attttctccg gcatgatgtg tgtgtggatg tctattttca ttggcagttt
     3001 gttaattggt aaattgggta ttggttaatt ggtagattcc gaaagtacaa atgtctttac
     3061 aggtttgaag cctgatcaac caattaacta tttaaccaat taacccttct ttactggtgg
     3121 ctccttcact gccccgcatt tattgcaggt tcttttcaat gtgtcttccc agaaccggt
//
LOCUS       DAORLS010000334         3178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16667_length_3178_cov_1.950688, whole genome shotgun sequence.
ACCESSION   DAORLS010000334 DAORLS010000000
VERSION     DAORLS010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16667_length_3178_cov_1.950688"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1265)
                     /gene="rocD"
                     /locus_tag="PK977_17705"
     CDS             complement(30..1265)
                     /gene="rocD"
                     /locus_tag="PK977_17705"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRF20019.1"
                     /translation="MQTLINMSEKTQYYLDLEENSGAHNYHPIPVVLTRGKGPHVWDV
                     DGKRYYDFLSGYSAVNQGHCHPKIVEALTDQAQKLTLTSRAFHSDLLGEFAKYITDYF
                     GYEKVLPMNAGAEGVETAIKLCRKWAYEIKGVEENKAVIIVCENNFHGRTTSIISFSS
                     DPSAKKNFGPYTPGFKAIPYNNIPALAEALKDKNIAGFLVEPIQGEAGVVVPDEGYLA
                     KAKTLCEEANVLFIADEIQTGLCRTGKMLACDHENVRPDILILGKALSGGMMPVSVVL
                     CDNEIMMTIKPGEHGSTYGGNPLACRVAVTALQVLKEESMADNATQMGELLRRELKAL
                     HSPHIAIVRGKGLLNAIVIKHPDKEAAWKLCLELKENGLLAKPTHGDKIRFSPPLVIN
                     EEQIMECVEIIRKSLKVLD"
     gene            complement(1570..1926)
                     /gene="queD"
                     /locus_tag="PK977_17710"
     CDS             complement(1570..1926)
                     /gene="queD"
                     /locus_tag="PK977_17710"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRF20020.1"
                     /translation="MEIYKEFAFDAAHFLPNVPEGHKCKNIHGHTYHLRVYVKGTPGE
                     HTGWIIDFKELKDVVKPIVDELDHTLINNISGLENPTAENITIWCWNRIKPLVPNLSR
                     IELKETPTSGVMYSGE"
     gene            complement(1991..>2692)
                     /gene="ispE"
                     /locus_tag="PK977_17715"
     CDS             complement(1991..>2692)
                     /gene="ispE"
                     /locus_tag="PK977_17715"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRF20021.1"
                     /translation="ESRNAKTAVHLSTSGEPVEGRPANNLCVRAYRLLQKDFPSLPPT
                     LIHLHKVIPAGAGLGGGSANASFTLQMINEIAGLGLNQQQLLHYAAQLGSDCPFFIIN
                     KPCYATGRGEILEEFELDLSTYSIVLVNPGIHVNTGVAFTGIVPAMPAYSIKEILQQP
                     VSQWKYKLQNDFEKTVFKTYREIVDIKDSLYQQGALYASMSGSGSSVYGIFEKDKVPV
                     TSFPADYFVKILPGK"
     assembly_gap    2693..2719
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2879..>3178)
                     /locus_tag="PK977_17720"
     CDS             complement(2879..>3178)
                     /locus_tag="PK977_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRF20022.1"
                     /translation="IDSRTSDAIALAVRFGCPIYTYENILESAGILMEETGTGKKKKS
                     KQEVVVEDEGPTGHEDLKTMTIEELNNLLNEVLDNEDYIRAIAIRDEINSRKKNK"
BASE COUNT          832 a          701 c          669 g          949 t
ORIGIN      
        1 ggttttgtgg caaataagta agctaacagt caatccagca ccttcaaact cttcctgata
       61 atttccacac attccatgat ctgttcttca ttgatcacta aaggagggga gaaccttatc
      121 ttatctccat gtgttggttt agccaataac ccattctctt ttaattccag gcaaagtttc
      181 catgcagcct ctttatccgg atgtttgatc acaatggcat tcagcagacc tttacctctc
      241 actatggcaa tatgaggtga gtgcaaagct ttcagttccc tgcgtagtag ctcacccatc
      301 tgcgttgcat tatcggccat actttcctct ttcaaaacct gcagggccgt cacggctaca
      361 cgacaggcta aaggattacc accgtaggtg gaaccatgtt caccaggttt gatggtcatc
      421 attatttcat tatcacacaa caccacactt accggcatca taccaccgct taacgctttg
      481 ccaagtatca gaatatctgg tcttacattt tcatgatcgc aggccagcat tttgccggta
      541 cggcaaagac cggtttgaat ttcatcagca atgaataata cgtttgcttc ttcacaaagt
      601 gtttttgctt tagcaagata gccttcatca ggcactacta cgccggcttc gccttgtatt
      661 ggttcaacga ggaagcctgc tatattttta tcttttaacg cctcggccaa agccggaata
      721 ttattgtagg gaatagcttt gaatcccggt gtgtaaggac caaaattctt tttggctgaa
      781 ggatcgctgc taaaagaaat gattgaagtg gtacgtccat gaaaattgtt ttcacaaact
      841 atgatcaccg ctttattctc ttcaacacct tttatttcat aggcccattt gcggcatagt
      901 ttaatcgccg tttccacacc ttcggcaccg gcattcatgg gcagcacttt ttcgtagccg
      961 aaataatctg tgatgtattt ggcaaattct cccagcaggt cgctgtgaaa ggcacggctg
     1021 gtcaatgtaa gtttttgtgc ctggtctgtc agcgcctcaa caatttttgg gtgacaatgt
     1081 ccctggttta cggcggaata gccgctcagg aaatcgtaat agcgtttgcc atccacatcc
     1141 catacatgag ggcctttgcc gcgggtaagc accacaggaa tagggtgata gttatgggcg
     1201 ccgctgtttt cttccaggtc aagatagtat tgagtctttt cagacatatt gattagtgtt
     1261 tgcatagttt aaaaattttg ggttcaggac agcaatgaag ggatacagcg atacatcctg
     1321 ccggttcagg aaggaaaaga aggcgttaac ctcgatggtt gaggaggtca agattttccg
     1381 agaggaatga tatagtaatt gaaattagca tgaatgtgcc tgcaaggtac aaaatacagc
     1441 cgaacttttt actgatgtgt tacaatcctg gccactgaaa cctgtcaacc ggacaggcgg
     1501 gcattgaaat tgtgatttta atgtaaccag ggtttcagtg tttctgtggc gtttctacaa
     1561 tcacattagt tattccccgg aatacattac cccactagta ggtgtttctt tcaattcaat
     1621 acggctcagg ttgggtacca ggggtttgat acggttccag caccagatag tgatattttc
     1681 tgcagtgggg ttttcaaggc cactgatatt attgatcagc gtatgatcca gctcatctac
     1741 aataggtttc actacgtctt tcaactcttt aaaatcaatg atccagccgg tatgttcccc
     1801 gggggtacct tttacataca ccctcaggtg ataagtatgt ccgtgaatat tcttgcattt
     1861 atgcccttcg ggaacattgg gcagaaaatg ggccgcatca aaagcaaact ctttgtagat
     1921 ctccataccg cttcaaattt gttgcaaaag taacggtaca ggatttatca tagctgagtg
     1981 agtgaaaaca ttattttccg ggtaaaattt tcacaaaata atcagctggg aatgaggtca
     2041 caggcacttt atctttctca aaaattccat atacgctact gccactgccg ctcatagatg
     2101 catagagtgc cccctgctgg tacaggctgt ctttaatgtc aactatttcc cggtaggttt
     2161 taaaaacagt cttttcaaaa tcattctgca gtttgtattt ccattgactg accggctgct
     2221 gcagtatttc tttgattgaa taggctggca ttgccggaac gatacctgta aaagctacac
     2281 cagtatttac atgaataccc gggttgacaa gaactatgct gtatgtagaa aggtccagtt
     2341 caaattcttc taaaatctct ccccggcctg ttgcatagca aggtttattg ataataaaaa
     2401 agggacagtc actgccaagt tgtgcagcat aatgtaaaag ttgctgctga tttagtccga
     2461 ggcctgcaat ttcgttgatc atttgcagtg taaaagatgc attggcactg ccgccaccca
     2521 ggcccgcacc cgccggtatc actttgtgca aatgaatcag cgtaggtggt aaagaaggaa
     2581 aatctttttg tagtaaacga taggcccgca cacaaaggtt attcgcaggc ctgccttcta
     2641 ccggctcacc actggtactt aagtgtacgg ctgttttggc attcctgctc tcnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnt atccgtaaga ccgatgggat agaagacagt ttcaaggtca
     2761 tgataaccat cgctgcgttt acgcaaaata tgtaagccaa gatttatttt gcaattggga
     2821 aaactgacca aggatcaata ggattttcag gtaaaatgga aataaatatt gaaagaagct
     2881 atttgttctt tttccggctg ttgatctcat ctcttatggc gatggcacgg atataatcct
     2941 cattgtccag tacttcattc agcaggttat tcaattcctc aatggtcatg gtcttcaggt
     3001 cttcatgacc ggttggccct tcgtcttcca ccacaacttc ctgtttagac tttttctttt
     3061 tgccggttcc tgtttcttcc atcaggatgc cggcactttc caggatgttt tcataggtat
     3121 aaatgggaca accaaaacgt acagccagtg caatcgcatc tgaggtacgg gaatcaat
//
LOCUS       DAORLS010000335         3159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16798_length_3159_cov_1.967461, whole genome shotgun sequence.
ACCESSION   DAORLS010000335 DAORLS010000000
VERSION     DAORLS010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16798_length_3159_cov_1.967461"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2204)
                     /gene="thrA"
                     /locus_tag="PK977_17725"
     CDS             complement(<1..2204)
                     /gene="thrA"
                     /locus_tag="PK977_17725"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRF20023.1"
                     /translation="MQVLKFGGTSVANAENIKKVVAIVKEAVKKEKTVVVVSALGGVT
                     DLLLGAASLAADGNETYKDKLAVVEQRHLDAVKELIPVATQSQLLSLVKKSCNEIEDI
                     CNGIFLLRELTPRTKDRVASYGEWLSSQIITARFNIEMVQAEWKDARELIRTNSSFTA
                     AEVDFVISNKKVLGYFSSTPGDLFIVPGFIASDSNGITTTLGRGGSDYTAAIIAAAMD
                     ASVLEIWTDVSGMMTADPRLTNNAKIIPHISYQEAMELSHFGAKVIYPPTIQPVMGKG
                     IPVWIKNTFSPEDEGTLIETTATGNTDIVRGISSINNIALISLEGSGMIGIPGFSKRL
                     FEALSNEKINVILITQSSSEHSITVGIDAAYAGKAKQATDAAFANEIALHKVEPLKVE
                     NELSIVALVGENMKNHTGISGRMFGAMGKNGVNIRAIAQGSSEKNISAVIATKDVKKA
                     INVLHEEFFETTYKQVNLFIIGTGNVGSKLLQQLQQQFVFLQKHLRLQVKVTGLCNSR
                     KMVLDEEGIDLSNWKERLDNGQLALLHQFAEEIISRNLRNAVFVDVTANADVADIYSK
                     LLEKSISVVACNKVAASSAYSNYKKLKDLAREYNSQFLFETNVGAGLPIIATLNDLVH
                     SGDRIHKIQAVLSGTLNFVFNNYDGEQKFAEVVRQAQKEGYTEPDPRLDLGGTDVMRK
                     ILILAREAGYQMDMEQVVNQSFMPEKCMQGSVNNFYQAMLEEEEHFKKLLNKA"
     gene            complement(2419..>2874)
                     /locus_tag="PK977_17730"
     CDS             complement(2419..>2874)
                     /locus_tag="PK977_17730"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRF20024.1"
                     /translation="DLMEHNVNEKDVVIGIAASGTTPYVIGALEECRRRGIVTGSISC
                     NPGSPVSAAADFPIEVVVGPEFVTGSTRMKSGTAQKLVLNMISTTVMIQLGRVEDNKM
                     VNMQLTNDKLVDRGTKMLMERIKMDDYEKAKELLLTHGSVKKAADSFGG"
     assembly_gap    2875..2920
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2921..>3159)
                     /locus_tag="PK977_17735"
     CDS             complement(<2921..>3159)
                     /locus_tag="PK977_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRF20025.1"
                     /translation="INKEDKTVPEAVAKAIPQIERLAEVASDKMLMGGRMFYIGAGTS
                     GRLGIVDASECPPTYGVPYGLVIGIISGGEKAITTA"
BASE COUNT          847 a          761 c          552 g          953 t
ORIGIN      
        1 gctttgttta gcagtttttt gaaatgttct tcttcttcca gcatcgcttg gtaaaaatta
       61 ttaactgacc cctgcatgca cttctccggc ataaaagatt ggttaaccac ctgctccata
      121 tccatctggt atccggcttc ccgggcaagt atcagtatct ttctcataac atcagttccg
      181 cctaaatcaa gccttggatc tggctcagta taaccttcct tttgcgcctg tcttactact
      241 tccgcaaatt tttgttctcc atcatagtta ttgaaaacaa aattaagggt accactaagc
      301 acagcctgaa tcttatgtat tcggtcgccg ctatgtacaa ggtcgttcaa tgtagcaata
      361 atgggcaagc cggctccaac atttgtttca aacaaaaact ggctgttata ctccctggcc
      421 aggtctttta gctttttgta attgctataa gcagacgatg cagctacttt attacatgct
      481 accacagaaa tacttttctc gagtagttta gaatatatat ctgctacatc ggcattggct
      541 gttacatcta caaaaacagc attgcgcaga ttgcgggaaa ttatctcttc tgcaaattga
      601 tgcaacaaag ccagttgccc attgtcaagt ctttctttcc agtttgacag atcaatacct
      661 tcttcatcaa gtaccatttt acggctgtta cacaggccag ttactttaac ctgcaacctg
      721 agatgttttt gcaaaaaaac aaactgctgt tgcagttgct gtaaaagctt tgaccctaca
      781 ttgcctgtac caataataaa aagattgacc tgtttgtaag ttgtttcaaa aaactcttca
      841 tgtaatacat tgatagcttt cttcacatct ttagtggcta tcactgctga gatgtttttt
      901 tctgaagacc cctgtgcgat agcccggata ttgacaccgt tttttcccat ggcgccgaac
      961 atccggccac ttataccggt atgattcttc atattctccc cgacaagagc tacaatcgaa
     1021 agctcattct ccactttaag cggctctact ttatgtaagg caatttcatt agcaaaagct
     1081 gcatccgtag cctgtttggc tttgccggca taagccgcat caataccgac ggtaatagag
     1141 tgctcagatg aactttgtgt aataagtata acattgatct tttcattact cagcgcttca
     1201 aaaagacgtt ttgaaaaacc gggaatacct atcataccgc taccttccag gctgatcaaa
     1261 gcaatattat tgatgctgga aatacctctg actatatcgg tattaccggt agcggtggtc
     1321 tcgattaagg taccctcatc ttcaggcgaa aaagtattct tgatccaaac agggattccc
     1381 ttacccatca ccggctgaat agttggcggg taaattacct tagcgccaaa atgcgataac
     1441 tccattgcct cttggtaaga aatatgcgga ataatcttcg cattattggt caaccgggga
     1501 tcggctgtca tcataccgct gacatctgtc cagatttcca gtacagaagc atccatggca
     1561 gcagcgataa tggccgcagt ataatccgat ccccctctgc ccaaggtcgt tgtaatgcca
     1621 ttactatccg aagcaataaa gcccggtacg ataaaaagat caccgggagt tgatgagaag
     1681 taacctaaaa ccttcttatt acttattaca aaatcaactt cagcggcagt aaaagatgag
     1741 tttgtccgga tcagttctct tgcatccttc cattcagcct gtaccatttc aatgttaaaa
     1801 cgggcagtta tgatttgtga agaaagccat tcaccataac tggccaccct atctttggta
     1861 cggggtgtta gttccctcaa taaaaaaata ccattacaga tatcctcgat ctcgttgcaa
     1921 ctctttttca caaggctcag cagttgactt tgtgttgcaa ccggtatcag ttcttttacc
     1981 gcatccaggt ggcgctgctc cacaacagcc aacttatcct tatatgtttc attcccatca
     2041 gcggctaagg atgcagcccc tagtaaaaga tcggttacac ctcctaacgc cgacaccact
     2101 actactgttt tttctttttt tacagcttct tttacaatcg caacaacctt tttaatattt
     2161 tctgcattgg caacggaggt gcctccaaat tttaagacct gcatgaccgt agtttaaata
     2221 agtaaatgat ttaaaaagct cacagttttt tgaactgcta tagcataatg cccatgtgat
     2281 gatatgaaaa tatactccgc tagagcggag ttgtggtagt tgtaataaaa ctaaccttag
     2341 cagaaatgtt atgtgaaata gtagatacca cgttactcag attagcacaa tatacgcagc
     2401 ttatccgaaa aacaaaattt atccgccaaa tgaatcagcg gctttcttta cactcccatg
     2461 ggttagcagt aattctttcg ctttttcgta gtcatccatc ttaattcttt ccatcagcat
     2521 tttagtacca cggtcaacca acttatcgtt agttagttgc atattcacca tcttattatc
     2581 ctctactctt cccagctgta tcataaccgt tgtagagatc atgttcagaa ccaatttttg
     2641 tgctgttccg cttttcattc gggtactgcc ggttacaaat tcagggccta ctaccacctc
     2701 aatcggaaaa tctgcagccg cactgaccgg agaacccggg ttacaggaaa tactgcctgt
     2761 tacaatgcct cttctacggc attcttccaa tgctcctatt acataaggag tcgttccact
     2821 tgcagcaatt ccaatgacta catccttttc gttaacatta tgctccatca aatcnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcagttgtaa tagctttttc
     2941 cccaccggat ataataccga tcaccaaccc gtaaggcact ccatatgtcg gaggacattc
     3001 gctggcatca acaatcccca acctgccact tgtgccggca ccgatgtaaa acatcctgcc
     3061 acccatcagc attttgtctg atgccacttc tgccagtctt tcaatctggg gtatcgcttt
     3121 tgcaacagct tcgggaacgg tcttgtcttc tttattgat
//
LOCUS       DAORLS010000336         3137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16948_length_3137_cov_2.682998, whole genome shotgun sequence.
ACCESSION   DAORLS010000336 DAORLS010000000
VERSION     DAORLS010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16948_length_3137_cov_2.682998"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..926
                     /locus_tag="PK977_17740"
     CDS             279..926
                     /locus_tag="PK977_17740"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF20026.1"
                     /translation="MKKSIVFLLAIITCFSVTAQTSKAKVGLVAGATISNMYGNVTGV
                     DVDYKSYAGYTFGMVVDAPLKKGHFSFQPGLHYTQKGATTLDVKNQEDYYALRYADLV
                     ANLVYNTHGDKGGNFYFGAGPQLGANLPSKKVTVFKPGDTKSESNIIFGDDVASDFRG
                     IDFGANIIAGYTMKCGVTFAANYNFGVRNLIPSGKNNDDKLRNSQIGIRVGYFFK"
     gene            1045..2583
                     /gene="lysS"
                     /locus_tag="PK977_17745"
     CDS             1045..2583
                     /gene="lysS"
                     /locus_tag="PK977_17745"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRF20027.1"
                     /translation="MSTAHLSEQEQLRREKLAELIMMGIEPYPAPLFPVKTNSVYIKE
                     NFREETKDQFTDVCIAGRIMSVRDMGKANFAVIQDGHGRIQLYIKRDDICPGDDKTLY
                     DVVWKKLTDIGDIIGVKGYVFTTKTGETSIHVKELTILCKSLRPLPIVKEAEGQTFDA
                     VTDPEFKYRQRYADLIINPAVKETFVKRTKMINSIREFLNNQGALEVDTPVLQSIPGG
                     AAARPFITHHNALDIPLYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRTHNPEF
                     TILEFYTAYKDYFWMMETTEQLLEKTALDVCGTTDVQVGDKTISFKAPFKRISIFDAI
                     KEHTGFDVSRMNEEQLRSVCKQLNIHVDNTMGKGKLIDEIFGAKCEKSYVQPTFIIDY
                     PVEMSPLTKKHREKQGLVERFELMINGKEIANAYSELNDPIDQRERFEEQVKLMERGD
                     DEAMFIDYDFLRALEYGMPPTSGIGIGIDRLCMLLTNQVSIQDVLLFPQMRPEQKLEQ
                     KEEA"
     gene            2643..3059
                     /locus_tag="PK977_17750"
     CDS             2643..3059
                     /locus_tag="PK977_17750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRF20028.1"
                     /translation="MTKQELLKELQQETYVALKPSPVHGIGVFAIRDIPKGCRSIFSK
                     AAGEWIKLSFEEVEQLPAHSRNLIETYCLYDETHYFVPDYGFKLMDLVLYLNHSHEPN
                     IASINDGEHFEALRDIKEGEELLVNYGTIAEGLENY"
BASE COUNT          929 a          665 c          675 g          868 t
ORIGIN      
        1 taataccaga accccggccc aaccggggtt ctttcgtaaa aaaggcttag ttggccgggt
       61 ttgtccttta aaatagcatc cacaaggctt ggcacggtac ctcctaacca gtacctttgc
      121 cccgcccaac agggctttta tgggtataaa aaggggttcg gggatccaga aatatatttt
      181 agcccctttc acccggttaa ctgaaaaata gtattttaga gccggttttg gatggggtac
      241 accccgctct gagccgtacg ataaccaaaa taactgatat gaaaaaatcg attgtttttc
      301 ttctcgcaat cattacatgc ttttccgtaa ctgcgcaaac ttctaaagct aaagtaggcc
      361 tcgttgccgg agcaactatt tccaatatgt acggaaatgt aacgggtgtt gatgtagatt
      421 ataaatctta tgccgggtac acttttggta tggtggtaga tgctccttta aaaaaaggac
      481 attttagttt ccagcccggt ctgcactaca cacaaaaagg tgctaccaca ctggatgtaa
      541 aaaaccagga agactactat gctttacgct atgctgacct ggtagcgaac ctggtatata
      601 atacacatgg tgacaaaggc ggtaattttt attttggtgc cggtccacag ttaggtgcga
      661 accttccttc taaaaaagta acagttttca aacccggtga tactaaatct gaatccaata
      721 taatctttgg cgatgatgta gcttccgatt tccgcggtat tgattttggc gccaatatta
      781 ttgcaggata taccatgaag tgtggcgtta cttttgctgc caattacaat tttggtgttc
      841 gtaacctgat tccttccgga aagaataatg atgataaact cagaaacagc cagatcggca
      901 tccgggtggg ttatttcttt aagtaaatac aaactctttt tatagcgaaa ccaccctgtt
      961 agggtggttt ttttatgcct tttgttttac ccttcgtttt aaccataacc agtacctttg
     1021 cccccttaaa attatcagtc attcatgagc actgcacatc tttctgaaca agaacaactt
     1081 cgccgggaaa aactggccga actgatcatg atgggcatcg aaccctatcc tgccccgctt
     1141 tttcccgtta agaccaactc cgtttatata aaggagaact tcagggaaga aaccaaagac
     1201 cagtttacag atgtatgcat cgccggccgc attatgagtg taagagatat gggcaaggcc
     1261 aattttgcag taatacagga tgggcatggc cgcattcagt tatacattaa aagagatgat
     1321 atctgccccg gtgacgacaa aactctctac gatgtagttt ggaaaaagct gaccgatatc
     1381 ggtgatatca ttggtgtgaa aggctatgta tttaccacca aaaccggtga aacttctatc
     1441 catgtaaaag aactgaccat actatgtaaa tcgctccgtc cgctgccgat agtaaaggaa
     1501 gcagaaggac agacctttga tgctgtaaca gatcctgaat tcaaataccg ccagcgttat
     1561 gctgacctga tcattaatcc tgcggttaaa gaaacttttg tgaagcggac aaaaatgatc
     1621 aactccatcc gtgagtttct caataaccag ggcgcactgg aagtggatac accggtactg
     1681 caaagtattc ccggtggtgc agccgcaagg ccttttatta cccatcacaa tgcactcgat
     1741 attcccttat acctgcgtat tgccaatgaa ctttacctga aaaggctgat cgttggtggc
     1801 ttcgactggg tttatgaatt cagccgcaac ttccgcaatg agggaatgga ccgcactcat
     1861 aatcctgaat ttaccatcct tgaattttac acagcgtaca aagactattt ctggatgatg
     1921 gaaaccaccg aacaactgct ggaaaaaact gccttggatg tttgtggtac aacagatgta
     1981 caggtcggcg ataaaactat ttctttcaaa gcaccgttca aacgtatcag catttttgat
     2041 gcaataaaag aacataccgg ttttgatgta agccgaatga atgaagaaca actccgttcc
     2101 gtttgtaaac aattgaacat ccatgtagac aatacaatgg gcaagggtaa actgattgat
     2161 gagatcttcg gggcgaaatg tgaaaagagt tatgtgcagc caactttcat cattgactac
     2221 cctgttgaga tgagcccatt gactaaaaaa cacagggaga agcagggact ggtggaaaga
     2281 tttgaattaa tgatcaatgg aaaagagatt gctaatgcct acagtgaact gaacgacccg
     2341 attgaccagc gggaacgttt tgaagagcag gtaaaactga tggaaagagg tgatgacgaa
     2401 gcaatgttta ttgactatga ttttctccgg gcactggaat atggtatgcc gcctacatcc
     2461 ggtattggta tcggtatcga cagactttgt atgctgctga ccaaccaggt gtcgatacag
     2521 gatgttctgc tttttccgca gatgcggcct gagcaaaagc tggaacaaaa agaagaagct
     2581 taataaatct tcattcgaaa tcctgtggct cttccacagg attttttctt gaaacaaaga
     2641 atatgaccaa acaagaactg ctcaaggaat tacaacagga aacttatgta gcgctgaaac
     2701 cttctcccgt tcatggtatt ggtgtttttg ctattcggga tatccccaag ggctgccgta
     2761 gtatcttttc caaagctgcc ggtgaatgga tcaaactttc gtttgaggag gtggagcaat
     2821 tacctgccca tagccgtaat ttgattgaaa cctattgcct ttatgatgag acccattatt
     2881 ttgttcccga ttacggtttc aaactgatgg atcttgtttt atacctgaac cactctcatg
     2941 aacctaatat tgcttccatc aatgacggtg agcattttga agcattgagg gatataaagg
     3001 aaggggaaga attattggtt aattacggaa ctattgctga agggctggaa aactattaac
     3061 ccacaaacaa atccgtatac aacttaccac ccggcaccac tgcgtattta tccaaatcag
     3121 taatgccttc tttggcc
//
LOCUS       DAORLS010000337         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16960_length_3135_cov_1.404870, whole genome shotgun sequence.
ACCESSION   DAORLS010000337 DAORLS010000000
VERSION     DAORLS010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16960_length_3135_cov_1.404870"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>368
                     /locus_tag="PK977_17755"
     CDS             44..>368
                     /locus_tag="PK977_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20029.1"
                     /translation="MKTNRKTNIYRLDITEIKHQKEEKLTSGVVFFGNPFIYGKVNNF
                     YQATLGFGQQLILGQKGNKNGVAVTAIYNGGLALGLLRPYYVEVEDPLTGENKVIKYS
                     TQDSAL"
     assembly_gap    369..385
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <386..626
                     /locus_tag="PK977_17760"
     CDS             <386..626
                     /locus_tag="PK977_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20030.1"
                     /translation="ILGSGGFGKGWSEMKMKPGAFVKTALRFDYGRFNEVVSGIEIGL
                     SAEFYADKIQIMANQPKDKQLFFQGYISLLFGRRK"
     gene            645..1574
                     /gene="lipA"
                     /locus_tag="PK977_17765"
     CDS             645..1574
                     /gene="lipA"
                     /locus_tag="PK977_17765"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRF20031.1"
                     /translation="MTELPVVPAATDNLRVKKPDWLRVKLPIGENYKHVRGLVDNHKL
                     HTICESGNCPNMGECWGAGTATFMILGNTCTRSCGFCAVATGRPEEVDWDEPQRVAEA
                     IHLMKVKHAVITSVDRDELKDGGSIIWYNTIKAVKSLNPDTTLETLIPDFKGEKENIQ
                     RIIDAAPEVVSHNIETVERLTRQVRIQAKYWRSMEVIRTLKEGGMRTKSGIMLGLGER
                     KEEVIQTLRDLRDNGCDVVTIGQYLQPTKKHLPVDRFVHPDEFAEYREIGYSLGLDYV
                     ESGPLVRSSYHSEKHVFADLGRQEWLQQKEQQA"
     gene            1580..2563
                     /locus_tag="PK977_17770"
     CDS             1580..2563
                     /locus_tag="PK977_17770"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRF20032.1"
                     /translation="MKKILVMGAMVILIAPFCFGQLKWVNVDSLFQPLPSSVHVYKTT
                     DPLDGKPNIAYYLEADLKHKQLDFTVDTTYQRRLTPSKFYEKNNRPLLVVNTTFFSFA
                     TNQNLNLVIKNKKLVSYNIHTINGRGKDTFTYRHPLGSAIGISKKRFADVAWLYTDSS
                     KKFAYAAHKSVQPVKDSTGRFSLNDFLKQLSPIAGVTRRGMKMHIRWKMQTAVGGGPV
                     LLQNGEIKISNNEEIKFAGKAINDRHPRTAMGYTRDNKLIILVVEGRNTGKAEGATLT
                     QLALKELGCWEALNLDGGGSSCMLVNGKETIRVSDSSGQRPVPAVFIIKGK"
     gene            2632..>3135
                     /locus_tag="PK977_17775"
     CDS             2632..>3135
                     /locus_tag="PK977_17775"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HRF20033.1"
                     /translation="MKKLFVPVMLLTSIAAILLIACSKEDSAGNTTLKVRLTDNPVNA
                     EEVNVDIQQVRVKFSEDSVQGWATLTTNAGVYNLLGLQNGVDTLLAVGTIPANTVKEI
                     RLVLGTDNTIKVAGNVYPLTIPSGAESGLKIKVNKTLQTSLDSLLIDFDAALSIHEIG
                     NGTYHLKP"
BASE COUNT          934 a          567 c          756 g          861 t
ORIGIN      
        1 gttcagaaca aatgggtatg gtgcctttta tgaactgggc aggatgaaaa cgaaccgcaa
       61 gacaaacatt taccggcttg atattactga gataaaacac caaaaagaag agaaactgac
      121 cagtggtgtt gtgttctttg gtaatccatt catttatgga aaggtgaaca atttttacca
      181 ggcaactttg ggttttggcc agcaattgat actcggtcaa aaaggaaata aaaatggggt
      241 agccgttact gctatttaca atggcggcct cgcattaggc cttttacggc cctattatgt
      301 ggaggtagaa gatccgttaa ccggagaaaa caaagtcatt aaatattcta cacaggatag
      361 cgccttatnn nnnnnnnnnn nnnnngattc tcggcagcgg cggctttgga aagggctgga
      421 gtgaaatgaa gatgaaaccg ggtgcttttg ttaaaacagc cctgcgtttt gattacggaa
      481 gattcaacga agtagtatcc ggtattgaaa taggactaag cgccgagttt tatgccgata
      541 aaatacagat catggccaat cagccaaaag acaaacaact tttctttcag ggctacatat
      601 ccctgttgtt tggccgacgt aagtaatggc cgtatttttg cccaatgact gagcttcctg
      661 ttgtacctgc agcgaccgat aatctgagag taaaaaaacc cgactggtta agggttaaat
      721 tgcctatagg agaaaattat aaacatgttc gtgggctggt ggataatcac aaactgcaca
      781 ccatttgtga aagcggcaat tgtcctaata tgggcgaatg ctggggtgcc ggcacagcca
      841 cgtttatgat actaggcaat acctgtaccc gtagttgtgg tttttgtgcc gtggcgacgg
      901 gccggccgga ggaggttgac tgggatgagc cgcaaagagt ggcggaggct attcacctga
      961 tgaaagtaaa gcatgcggtt atcacttctg ttgacaggga tgagttaaaa gacggtggtt
     1021 ctattatctg gtataatacg atcaaagcag taaaatcatt aaaccccgat acaacgctgg
     1081 aaacactgat tcccgatttt aaaggcgaaa aagaaaatat tcaacggatc attgatgcgg
     1141 ctcctgaagt ggtaagccat aatattgaaa cagtagaaag gctgacccga caggttcgta
     1201 tacaggccaa gtactggaga agtatggagg tgattcgtac actgaaagaa ggtggtatgc
     1261 gtaccaaaag tggcattatg cttggtctcg gtgaaagaaa ggaggaagtg attcaaacct
     1321 tgagagatct gcgggataat ggttgtgatg tcgttacgat tggtcaatac ctgcagccta
     1381 caaagaagca tttaccggtt gatcgttttg tgcatcctga tgagtttgcg gaataccggg
     1441 agatcgggta tagtcttgga ttggattatg tagaaagtgg cccgctggtt cgttcttctt
     1501 atcacagcga gaaacatgtt ttcgctgacc ttggccggca agaatggttg caacaaaaag
     1561 aacagcaagc ctgataatca tgaaaaagat acttgttatg ggagcaatgg tcattctgat
     1621 tgctcctttt tgttttggcc agttgaagtg ggtgaatgtg gattcactgt ttcagccatt
     1681 accatcctca gtacatgttt ataaaacaac tgacccgttg gatggtaaac ccaatattgc
     1741 ttactacctg gaagcagacc tgaaacataa acaactggat tttactgttg atactactta
     1801 tcaaagaagg ttaactccat caaaatttta cgagaagaat aaccggcctt tgttggtggt
     1861 caatactact tttttctcct ttgccactaa tcagaatttg aacctggtaa taaaaaacaa
     1921 aaaactagtc agctataata tacatacgat taatggaagg ggaaaagata cgtttacgta
     1981 taggcacccg ctgggcagtg ccatcggcat atctaagaag cgatttgcgg atgtagcatg
     2041 gttgtatact gattcgtcaa agaaatttgc ttatgcagcg cataaatcgg ttcagcctgt
     2101 taaagattca acaggcagat tttctttaaa tgattttttg aaacaacttt ccccgattgc
     2161 cggtgtaacc cgtcgtggta tgaaaatgca tattcgttgg aaaatgcaaa ctgctgttgg
     2221 aggcggacct gtattgctgc aaaatggtga aattaaaatc tccaataatg aagaaataaa
     2281 atttgccggt aaagccatca atgataggca tccccggaca gcaatgggtt acacccggga
     2341 taataaactt atcatcttag ttgttgaagg ccgcaatacg ggtaaagccg aaggtgccac
     2401 ccttacacag ttggcattaa aagagcttgg ttgttgggaa gcccttaacc tggatggcgg
     2461 cggcagcagt tgcatgttgg tcaatggtaa agaaacgatc agggtctctg atagttccgg
     2521 tcaaaggcca gtacctgcag tatttattat caagggtaag tagagttttg atttttgcac
     2581 attttgactc gttggcacgg ttttttggct tttattttca aacaatgtat tatgaaaaaa
     2641 ttatttgtgc cagtgatgtt actgacctcc attgccgcta ttctattgat tgcctgctcg
     2701 aaagaagata gtgcaggcaa tacaactttg aaagttagat tgacagataa tccggttaat
     2761 gctgaggagg tgaatgttga tattcagcag gtaagggtaa aattcagtga ggattctgtt
     2821 cagggctggg ctacattaac tacaaatgcc ggcgtttata atcttcttgg tttgcagaac
     2881 ggagtggata cgttgctagc ggtaggtact attccggcaa atacggtaaa agagattcgc
     2941 cttgtattgg gaactgacaa tacgatcaaa gtagccggaa atgtttatcc gctgacaatt
     3001 cccagcggtg ctgaatccgg gttaaagatc aaggtgaata aaacactgca aacatcactt
     3061 gattctctat tgattgattt tgatgcagca ttatcaatcc acgagattgg taatggaact
     3121 tatcacttaa agcct
//
LOCUS       DAORLS010000338         3134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_16961_length_3134_cov_1.643391, whole genome shotgun sequence.
ACCESSION   DAORLS010000338 DAORLS010000000
VERSION     DAORLS010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16961_length_3134_cov_1.643391"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>362)
                     /locus_tag="PK977_17780"
     CDS             complement(<1..>362)
                     /locus_tag="PK977_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRF20034.1"
                     /translation="TNDDELAVKLKMIANHGQSKRYYHDVVGCNSRLDTIQAAILDIK
                     LRHLDEYTIARRAAADFYDKSFAGNPKIKTPYRAPFSHHVFHQYTMILEGVDRDGLNQ
                     FLAEHKIPSMIYYPVPGH"
     assembly_gap    363..380
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<381..975)
                     /locus_tag="PK977_17785"
     CDS             complement(<381..975)
                     /locus_tag="PK977_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF20035.1"
                     /translation="MRPIQMVDTKTQYHKIKSDVDKEVLDVLESSAFINGKPVQDFAA
                     NLSAYLGVKHTIPCANGTDALQIAMMALGFQPGDEVITPSFTYIATTEVIALLKLTPV
                     FVEVDPKTFCIDPTALEKAITPKTKAIVPVHLYGHAAPMEEIMAIAKKYDLFVIEDNA
                     QAIGCDYTFSDGTKKKTGTIGHIGATSFYPSKNLGAFG"
     gene            1133..>1717
                     /locus_tag="PK977_17790"
     CDS             1133..>1717
                     /locus_tag="PK977_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRF20036.1"
                     /translation="MSIFLYNIFLLVYRFSLFAASLFNDKARKWITGRKGIFESLQTS
                     IPTSDKIIWMHCASLGEFEQGRPVLEKIKATYPGYKVLLTFFSPSGYEVQKNYRGADW
                     VFYLPLDGASNARRFLEITHPSLVIFVKYEFWYYYLKKIKYRKIPLILISAIFRKEMS
                     FFKWYGSIQRKILTRFDHLFVQDKNSKKLIDEISL"
     assembly_gap    1718..1732
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1733..2405
                     /locus_tag="PK977_17795"
     CDS             <1733..2405
                     /locus_tag="PK977_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRF20037.1"
                     /translation="CSVSGDTRFDRVIEIAENFNPIPEIEKFIQNNPVIVAGSTWPED
                     ENVLKTAFKSLNNNAARLIIVPHEIHEGHIKDLKTIFPDSVLFSTLKDGFKSPENNRI
                     LIIDNIGMLSRLYKYAGVAYVGGGLQSTGVHNVLEAAVYNKPVIIGPHYEKYQEAIDL
                     VNSGGGIVIKDAGALSDRFMQLLNNQNGIYDKTSHAAGTFVRANGGATGKILSFIQEN
                     RLLTN"
     gene            complement(2378..>2802)
                     /locus_tag="PK977_17800"
     CDS             complement(2378..>2802)
                     /locus_tag="PK977_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20038.1"
                     /translation="TVSVVQKNGQLDMVFDPMTEIPTLSHQPKDKKEINESGGQEESL
                     NDKLKQGKTELVEILKESPVKDLRKAIGINDRFLFINELFRGDESVYERSIKTINSFN
                     IYAEAEYWISRELKVKLGWNNDNPTVQHFDQLVKRRFS"
     assembly_gap    2803..2832
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2833..3096)
                     /locus_tag="PK977_17805"
     CDS             complement(<2833..3096)
                     /locus_tag="PK977_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20039.1"
                     /translation="MERIEALIQKLKQQFEQQADPVEMLGTVQLLQFELSKQQTQKQQ
                     SLGTAKVAVVLPAMSMSMTVVEDIPKAYEKYAPRPAEEQHVVKE"
BASE COUNT          880 a          618 c          608 g          965 t
ORIGIN      
        1 tatgtcccgg caccgggtaa tagatcatag atggaatttt atgttcggca aggaattggt
       61 ttaaaccatc cctgtctact ccttccagga tcattgtgta ctgatggaaa acatgatgag
      121 aaaatggcgc cctgtaaggt gtttttattt taggatttcc ggcaaaggat ttatcataaa
      181 aatcagcagc tgccctccgg gcaattgtat attcatcaag atggcggagt tttatatcca
      241 ggatagcggc ttgtattgta tctaacctgc tattacaccc cacaacatca tggtaatatc
      301 ttttactttg accatgatta gcgatcattt tcaatttaac cgccagttca tcatcgttgg
      361 tannnnnnnn nnnnnnnnnn caccaaatgc acccagattt ttcgaagggt agaatgatgt
      421 ggcgccaata tgcccgatag tacctgtttt tttcttcgtg ccatcagaga aggtataatc
      481 acaaccaatt gcctgcgcat tatcttcaat cacaaaaaga tcatactttt tggcaatggc
      541 cattatttcc tccatcgggg ctgcatgccc atacagatgg actggtacaa tagctttagt
      601 tttaggagta atggcttttt ccaaagcagt gggatcaata caaaatgttt taggatcaac
      661 ttctacaaat acaggtgtta gtttcagcaa ggctattact tccgtagtgg cgatataggt
      721 aaaggaaggt gttattactt catcaccggg ttgaaagcct aatgccatca tcgctatttg
      781 caaagcatcg gtaccgttgg cacagggtat ggtatgcttc acacctagat aagctgatag
      841 attggcagca aaatcctgaa caggttttcc attgatgaaa gccgaacttt ccaatacatc
      901 caacacctct ttatctacgt cggattttat tttatgatac tgggtcttgg tatcgaccat
      961 ctggataggt ctcatgggga aatgtttaag ttgaaaatac cagccggtca accccttttc
     1021 cattcaggtt caggcgattc ggtcaggcgg gcaaaaatag taaaatacag gtagtttacc
     1081 cgattgcttt tacatttgcg ctttacgata ctaccggcaa aaaacagaca tcttgtccat
     1141 ttttctttac aatatctttc tcctggtgta caggtttagc ttgtttgctg cttctttatt
     1201 taatgacaag gccaggaaat ggataacagg cagaaaagga atatttgaaa gtttgcaaac
     1261 cagtattccc acatcggata aaatcatctg gatgcattgc gcctccctgg gcgaatttga
     1321 gcaaggccgg ccggttttag aaaaaataaa agccacttac ccgggttaca aagtattact
     1381 aacctttttt tcaccttcag gatacgaggt acaaaaaaat tatagaggag ctgattgggt
     1441 attttatctt ccgttagacg gggcatctaa tgcccggcgc tttcttgaaa tcacccatcc
     1501 ttccctggtc atatttgtaa agtatgaatt ttggtattac tacctgaaaa aaatcaagta
     1561 cagaaaaata cccttgattc ttatatcagc catctttcga aaagaaatgt cattcttcaa
     1621 atggtatggc agtatacaac gaaaaatatt aacaagattt gaccatttgt ttgttcagga
     1681 taaaaactca aaaaaactga ttgatgaaat tagcctcnnn nnnnnnnnnn nnctgcagcg
     1741 tttccggtga tacccgtttt gacagggtaa ttgaaattgc agaaaatttt aaccctatac
     1801 cggaaataga aaagtttatt caaaataatc ctgtaatcgt tgcaggcagc acctggcccg
     1861 aggatgaaaa tgtattgaaa acggccttta aatctttaaa taacaacgca gcaaggctta
     1921 ttatagtgcc acatgaaatt catgaaggac atataaaaga cttaaaaaca atttttcccg
     1981 attcagtact attttctact ttaaaagatg ggtttaaaag cccggaaaac aacagaatac
     2041 tgatcattga taatatcggg atgttatcca ggttatataa atatgctggt gttgcctatg
     2101 tggggggtgg tttacaaagc accggtgttc ataatgtgct ggaagcagct gtatataata
     2161 aaccggtaat cattgggccc cattatgaaa aataccagga ggcaatcgac cttgtaaaca
     2221 gtggcggggg aatcgttatt aaggatgccg gtgcactttc tgaccgcttt atgcagctgt
     2281 taaataacca aaatggcatt tatgataaaa ccagccatgc agccggaacc tttgtaaggg
     2341 ctaatggtgg cgcaaccggg aaaatccttt cttttattca ggagaaccgc cttttaacca
     2401 attgatcaaa gtgttgtaca gttgggttat cattattcca gcccaatttt actttaagct
     2461 cccggctgat ccagtattca gcctcagcat agatattaaa actgttaata gttttgatac
     2521 ttcgttcata aaccgattca tctccacgaa atagttcatt aataaaaaga aaccggtcat
     2581 ttataccaat tgctttacgc agatctttta caggagattc ttttaatatc tcgaccaact
     2641 cggtttttcc ttgtttcagc ttatcattca gcgattcttc ctgtccgcca gattcgttga
     2701 tctccttctt atcttttggc tgatgagata gtgttggaat ttcggtcatg ggatcaaaga
     2761 ccatatctaa ttgtccgttt ttttgaacta ctgatacggt tcnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnttctttta ccacatgttg ctcttctgca ggcctgggtg catatttttc
     2881 atacgccttt gggatgtctt caacgacggt cattgacata gacatggctg gtaacaccac
     2941 cgctacttta gccgtaccaa ggctttgttg tttttgtgtt tgctgtttgg acagttcaaa
     3001 ctgtaataat tgcactgtgc ccagcatctc taccgggtcg gcctgttgct cgaattgttg
     3061 ttttaatttt tgtatcaggg cttctattcg ttccattgat ttatatttac cgcccgcaaa
     3121 ttgcgaccat ttaa
//
LOCUS       DAORLS010000339         3129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17004_length_3129_cov_1.960638, whole genome shotgun sequence.
ACCESSION   DAORLS010000339 DAORLS010000000
VERSION     DAORLS010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17004_length_3129_cov_1.960638"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    279..288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <289..1181
                     /locus_tag="PK977_17810"
     CDS             <289..1181
                     /locus_tag="PK977_17810"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRF20040.1"
                     /translation="LFKKSKKIYAVFNDLGGLSRSNEVKVNGFVIGTVYSLNKKDANL
                     DEIVATINLTEDVNIPRDSKAIITSPLVGSWYINIEKGESVEFLKPGDTLKSKLDVGI
                     LDDVKAQLTPTLTKVRNTLDTLNTVLGSVNGILNSEAKNNLQQTLANLNRASSALNGL
                     LDNQSGSLAKTLNNAEAISQSLKNNTEDITATIVNAKKASEKLAALEIQPTLDSLNSM
                     ISNLKVVASKLNSNEGTLGALMNDRTLYKKITDAILSAEILMDDMRAHPKRYVNFSIF
                     GRKDKGGALTSPSVKDSISK"
     gene            1188..>2363
                     /locus_tag="PK977_17815"
     CDS             1188..>2363
                     /locus_tag="PK977_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRF20041.1"
                     /translation="MHRFFSFVLLFTGLLLSTLSWGQVIPPPQPVTSSSTDSLLLVEI
                     LPGVRKLEFRKVNDSTQLQILAGNVKLKQGTSYFYCDSCVINNNTKTFEEWGNVHIND
                     SDTAHIYSSHLRYLMNKRIAYLDGGVRLTDGKGSLTTPDLEYDMTTDFGIYTHGGKVI
                     NKKTVLTSQEGYYYAGLRDVYFKKNVKLKDPAYYIETDSLLYNTESQTTRFIAETFIK
                     DSSGRTIKTKEGYYNIATGKAEFGQRPIIQDGKTTIIANRIAFDDSAGTSQAIGNVII
                     VDSAQGTTILAGEVYRDNKNDRLLATRKPLMIIKQDNDSIYVTADTLFSARLTDLYGK
                     KDSVVKDTVKGTKVVTVSNAKDSTNRYFEAYRHVRIFADSMQAVCDSLFYSFKDSTFR
                     "
     assembly_gap    2364..2404
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2405..>2759
                     /locus_tag="PK977_17820"
     CDS             <2405..>2759
                     /locus_tag="PK977_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA family protein"
                     /protein_id="HRF20042.1"
                     /translation="GDTVLIFTKNKKADRVKVFENSFLVSQTDPEVYNQIKSSRMDAY
                     FKEGTIDSVRAAGYAECIYYLQDEDSSYTGINESKCDIIDIYFGNEALQKVVFRSSVA
                     GTIWPIRQKDPKEMRL"
     assembly_gap    2760..2785
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2827..>3129)
                     /locus_tag="PK977_17825"
     CDS             complement(2827..>3129)
                     /locus_tag="PK977_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRF20043.1"
                     /translation="IVGSIGLLSSVVDNVPLVAAAQGMYGLDVYPTDAYFWEFLAYCS
                     GTGGSILIIGSAAGVAAMGIEKIDFIWYLKKIGWLALLGYFAGAVTYVLQHQFFGF"
BASE COUNT         1038 a          631 c          606 g          777 t
ORIGIN      
        1 gaacagggac aacagggaat ttctggaaat ggttgacaaa atggaaaaac aacgaatgag
       61 taagtaaaaa caaaaatacc cggcaaaaga ggatgaaaat tcatcctctt ttttgttggc
      121 gtaagttgtg tatatcgttt gtaaataatt ggctaatttg gcatctttgc ttggcctact
      181 ggctaacaaa cacaacctga tgaaaataag caacgaaaca aaggtcggca tacttactat
      241 ctcagccctt actgttctga tactgggttt taatttccnn nnnnnnnnac ttgtttaaaa
      301 agtcaaaaaa gatctatgct gtgtttaatg acctgggtgg cctctcccgt tccaatgaag
      361 taaaagtaaa tggctttgtc attggcactg tttacagcct gaataaaaaa gatgccaacc
      421 ttgatgagat tgtagccaca atcaacctca ctgaagacgt aaacataccc agagattcca
      481 aagcaattat cacatcaccg ttggtaggat cctggtatat aaatattgaa aaaggcgaat
      541 ccgttgagtt cttgaaacct ggtgatacat tgaaatcaaa attagatgta ggtattcttg
      601 atgatgtaaa ggcgcaactc acccctacgc ttaccaaagt aagaaacacc ctggacacac
      661 tcaatactgt attgggaagt gtaaacggca tattgaacag cgaagccaaa aacaacctcc
      721 agcaaacttt ggctaatctg aaccgggcca gcagtgcttt aaatggttta ctcgataatc
      781 aatccggttc actggctaaa actttgaata atgcagaggc tatctctcaa agtctcaaga
      841 acaatacaga agatataaca gctactatcg taaacgccaa aaaagcatca gaaaaattag
      901 ctgcactgga aattcaaccg acacttgact cactgaactc aatgatcagt aatttgaaag
      961 tggtagcatc caaattaaac agcaatgagg gaacattagg agctttgatg aacgacagaa
     1021 cgctttataa aaagatcacc gatgctatat taagcgctga aatattgatg gatgatatgc
     1081 gggcacatcc taaacgctac gttaatttct ccatctttgg ccggaaagat aaaggcggtg
     1141 ctctaacttc gccatccgtt aaagacagta tatcgaagta gcttataatg catagatttt
     1201 tttcctttgt tttactgttt acagggcttt tgctcagcac tttgtcatgg ggacaggtca
     1261 ttcctcctcc gcaacccgtc acctcgtctt ccactgatag cttactgctt gtggaaattt
     1321 tacctggtgt aagaaaactg gagttcagaa aagtaaatga ttctacccaa ctgcaaatac
     1381 tggccggtaa tgtaaaattg aagcagggaa cttcctactt ctattgtgac agttgtgtta
     1441 tcaataataa tacaaagaca tttgaagaat ggggcaacgt acatataaat gattcagaca
     1501 cagctcatat ttattccagc caccttcgct acctgatgaa taagcgaatt gcctatctcg
     1561 atggtggggt tcgcttaaca gacggaaaag gaagcctgac aactcctgat ttggagtatg
     1621 atatgacaac ggatttcggc atttacacac atggcggaaa agtcataaat aaaaaaacag
     1681 tcttaaccag ccaggaaggc tactattacg ccggtttaag ggatgtttat ttcaaaaaga
     1741 acgttaagct gaaagatcct gcctattata tcgaaaccga ttcattgctt tataatactg
     1801 aatcgcaaac cacacgtttc atcgcagaga cctttataaa agacagcagc ggacgaacga
     1861 taaaaaccaa agaaggctac tacaatatcg ctaccggtaa agcagaattt ggacaacggc
     1921 caataattca agatggtaaa acgacgatta tcgccaatcg cattgccttt gatgacagcg
     1981 ccggcacctc ccaggctatt ggcaatgtta ttatcgtgga ttcagcccag ggaaccacta
     2041 ttctggctgg agaagtttac agagataata aaaacgaccg attattggca acaagaaagc
     2101 cattaatgat catcaagcag gataatgatt ccatttatgt aactgcggac accttatttt
     2161 cggcaagatt aactgacctg tacggaaaaa aagattccgt agtaaaggat acagtgaaag
     2221 gaacgaaggt tgtcacggtc agcaatgcaa aagatagtac caaccggtat tttgaagctt
     2281 acaggcatgt acgaatcttt gctgattcta tgcaggccgt atgcgacagc cttttttatt
     2341 catttaaaga ttccactttt cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnggcgac acagtgctga tattcacaaa gaataaaaag gcggaccggg taaaagtatt
     2461 tgaaaatagc tttctcgtaa gccaaaccga cccggaagta tataaccaga tcaaatcaag
     2521 ccggatggat gcttatttta aagaaggaac tatcgactca gtaagagctg ccgggtatgc
     2581 agaatgtatt tactatctgc aggacgaaga cagttcctac accggtatta acgaatcaaa
     2641 atgcgacatt atcgatattt attttggaaa tgaagcattg caaaaagtag ttttcagaag
     2701 cagtgtggcg ggaaccatat ggccaatccg tcaaaaagat ccaaaagaaa tgcgattacn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnngaaag gagacctaaa tcgaaatacg aattgttcga
     2821 atgaggtcaa aatccaaaga actgatgctg caaaacatag gttaccgctc ctgcgaaata
     2881 tcctaacaat gccagccaac cgattttttt taaataccag ataaaatcaa tcttttcaat
     2941 tcccattgcg gcaactcccg ctgcagaacc aatgatcagt atgcttcctc cagttcccga
     3001 acaataagca aggaattccc aaaaatatgc atccgtcggg tacacatcaa gcccatacat
     3061 accctgcgcc gcagcaacca gcggcacatt gtctaccact gaagataaca atccaatact
     3121 gccgacaat
//
LOCUS       DAORLS010000340         3122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17051_length_3122_cov_2.203130, whole genome shotgun sequence.
ACCESSION   DAORLS010000340 DAORLS010000000
VERSION     DAORLS010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17051_length_3122_cov_2.203130"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PK977_17830"
     CDS             <1..723
                     /locus_tag="PK977_17830"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRF20044.1"
                     /translation="EVADALKGVDVPVLIKNPINPDLELWGGAVERVARAGVKQIGLI
                     HRGFSSYGNTEYRNAPMWHLAIDMKLKHPGMPLINDPSHICGRRDILLDIMQKAIDLD
                     YDGLMIETHIDPDNAWSDAKQQVTPEKLAEMLDHIIWRKEDVNSEEYHAALEALRQKI
                     NHLDDELMQILGQRMKIAEQIGRYKKENNITILQTNRWNEIIERASVKGEKIGLSKEF
                     ITRYLDAVHMESINHQKKVLDS"
     gene            772..1791
                     /gene="aroB"
                     /locus_tag="PK977_17835"
     CDS             772..1791
                     /gene="aroB"
                     /locus_tag="PK977_17835"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRF20045.1"
                     /translation="MTHVKVNFSSSATDFYFAYGISHLKKITDPKATVLITDENIYHA
                     HAIRFNGWNTIVLKPGEDYKVQATADAVIAKLIEMQADRMTTLVGVGGGVVTDLTGYV
                     ASIYMRGIRFGFIPTSVLGLVDASIGGKNGVDAGAYKNMVGTIRQPAFILHDMVFLNS
                     LPKQEWENGFAEIIKHACIKDAAMFRELEVNSLETYRGKKKAICTLVQRNAEIKIRVV
                     QKDEFEKGERRLLNFGHTLGHALETQYELLHGQAISIGMTYACHISEQLCGFKQTDEL
                     VSVLEKYNLPTYAAFNKQKVFNVLKMDKKRERKEMNYVLLEKIGKGVVKSISLKQLEK
                     IIGEL"
     gene            1792..3090
                     /gene="aroA"
                     /locus_tag="PK977_17840"
     CDS             1792..3090
                     /gene="aroA"
                     /locus_tag="PK977_17840"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRF20046.1"
                     /translation="MKVTIHPSQLKGAVQAPASKSSMQRACAAAIIRKGQTIIANPGH
                     SNDDKAALDIIKRLGAKTIFENGVWKVESGGVEPVEHEINCGESGLSIRMFTPLVALS
                     DKEITINGSGSLLTRPMDFFDEILPQLGVKVKSNNGKLPMIIQGPLQPKNIEVDGSLS
                     SQFLTGLLMAYAAAGAKDISIKVNNLKSKPYISLTLDVMKQFRLQLPENRDFEEFVFS
                     TGLSVEQSATQPVSYTVEGDWSGASFLLVAGAVAGPITVKSLDLTSAQADKAIIDALM
                     AANTGIAIDAKGITVHPGEMNGFYFDATDCPDLFPPLVVLAAYCNGKTTIKGVNRLTY
                     KESNRALTLQDEFDKMGVYIELNDDIMIVHGTGKVKGADVHSNHDHRIAMACSVAALK
                     ANSETIIEEAQAIKKSYPDFYEDLKKLGADVSLPFTHFNL"
BASE COUNT          991 a          542 c          753 g          836 t
ORIGIN      
        1 gaagtagcgg atgcattaaa aggagttgat gtgccggttt tgattaagaa cccgataaat
       61 cctgatttag aattatgggg cggtgctgtg gaaagagtgg caagagctgg tgtgaagcaa
      121 atcgggttga ttcaccgcgg ctttagttct tatggtaata cagaataccg caatgcgcct
      181 atgtggcacc tggcaattga tatgaagctg aagcacccgg gtatgccgct gatcaatgac
      241 ccatctcata tttgtggccg tcgtgatatc ttactggata ttatgcaaaa agctattgac
      301 cttgattatg acggactgat gatcgaaaca catattgatc ctgataatgc ctggagtgat
      361 gccaagcaac aggtcacacc ggaaaagctg gctgaaatgc tggaccatat tatctggaga
      421 aaggaggatg ttaattctga agaatatcat gcggcactgg aagcattgcg ccaaaaaata
      481 aatcacctgg atgatgagtt aatgcagata ctgggtcagc gtatgaagat cgctgaacaa
      541 attggccggt ataagaaaga aaataatatc accatccttc agaccaaccg gtggaatgaa
      601 attattgaaa gggccagtgt gaaaggagaa aagataggtt tgagcaaaga attcattacc
      661 cgctacctgg atgcggtgca tatggaaagt atcaatcacc agaagaaagt attggatagt
      721 taatatccac catatcttgc tgccatcctt gacgaagttt aaagaagaaa aatgacacat
      781 gttaaagtaa atttctcatc ttctgctacc gatttctatt ttgcttatgg aatcagtcat
      841 ttaaaaaaaa ttactgaccc caaagcaact gttcttatta ctgatgagaa tatttatcat
      901 gcgcatgcta taaggttcaa tggctggaat acaattgtac tcaaacccgg agaagattat
      961 aaagtacagg caacggcaga tgctgttatt gctaagctga ttgaaatgca ggctgaccgg
     1021 atgacaacgc tggtaggtgt aggcggagga gttgttactg atcttacagg ctatgtggca
     1081 tctatataca tgaggggtat ccggtttggc tttattccaa cttctgtttt gggtttggta
     1141 gatgcttcta ttggcggaaa gaacggggtt gacgctggag catataaaaa tatggtaggc
     1201 actatccggc agccagcttt cattttgcat gatatggttt tcctcaattc tttgccgaaa
     1261 caggaatggg aaaacggttt tgcggagatc attaagcatg cctgtataaa agatgctgcg
     1321 atgttccggg aactggaggt aaattcatta gaaacataca ggggcaaaaa gaaggcaatt
     1381 tgtacattgg tacaacgaaa tgctgaaata aaaatccggg ttgtacaaaa ggatgaattt
     1441 gaaaaaggcg aaagacggct gttgaatttt ggccacactc tgggacatgc actggagacc
     1501 caatatgaat tattgcacgg gcaggctatt tccatcggca tgacttatgc ctgtcatatt
     1561 tctgaacagt tatgcgggtt taagcaaacc gacgagttag tgagtgttct tgaaaagtac
     1621 aatttgccta cttatgcggc atttaataag caaaaagtat ttaatgtact gaagatggat
     1681 aagaagcggg agagaaaaga aatgaattat gtgctgttag aaaaaattgg caaaggagta
     1741 gtaaaatcca tctctttaaa acagttagaa aaaattatag gggaattata agtgaaggtt
     1801 acaatacatc catcgcaatt aaaaggggca gtgcaggcgc cagcttccaa aagctccatg
     1861 caaagagctt gtgcggcagc gataataaga aaagggcaaa ctattattgc caatcccggt
     1921 cactccaatg atgataaagc agcattggac atcattaaac gattgggtgc caaaacaatt
     1981 tttgaaaatg gagtatggaa agtagaaagc gggggagttg aaccagttga gcatgaaatt
     2041 aactgtggcg aaagtgggtt aagtattaga atgtttactc cactggtagc attgagcgat
     2101 aaagaaatta caatcaatgg ttcaggaagt ttgctcacca ggcctatgga ctttttcgat
     2161 gagatattgc cccaactggg ggtaaaagtg aaaagcaata atggtaagtt gccaatgatt
     2221 attcaaggtc cgttgcaacc aaaaaatatt gaggtggatg gttcactcag ttcacagttt
     2281 ttaacagggt tgttgatggc ttacgctgca gcgggtgcaa aagatatttc tattaaagtt
     2341 aataacctaa aaagtaagcc ctatatctcc ctgacattag atgttatgaa gcagttcagg
     2401 ctgcaattac ctgaaaacag ggattttgag gagtttgttt ttagcactgg gctatctgtt
     2461 gagcaatcag ccacacagcc ggtcagctac acggttgaag gtgactggag cggtgcttca
     2521 tttctgcttg tggcaggcgc cgttgccggg ccaataactg taaagagtct tgacttaaca
     2581 tctgcccagg cagacaaagc aattattgat gcattgatgg ctgccaatac cggcattgct
     2641 atcgacgcaa aaggaattac tgttcacccg ggtgaaatga atggttttta ttttgatgcg
     2701 acggattgcc cggatttatt tccgccactt gtggtactgg cggcgtattg caatggaaaa
     2761 acaactatca aaggagtaaa ccgcttaact tataaagaaa gtaaccgggc acttacttta
     2821 caggatgagt ttgataaaat gggagtgtat attgaactga acgatgacat aatgatagtc
     2881 catggaactg gaaaagtgaa gggagctgat gtacactcaa accatgatca ccgcattgcc
     2941 atggcctgtt cggtggctgc attaaaagcc aatagcgaaa caataattga agaagcgcag
     3001 gccattaaaa aatcatatcc tgatttttat gaagatttga aaaagctggg ggctgatgta
     3061 tctttacctt tcacacactt caacttataa actttgaact tattcggccg tcttttccgc
     3121 at
//
LOCUS       DAORLS010000341         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17121_length_3111_cov_1.821008, whole genome shotgun sequence.
ACCESSION   DAORLS010000341 DAORLS010000000
VERSION     DAORLS010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17121_length_3111_cov_1.821008"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    242..278
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            502..732
                     /locus_tag="PK977_17845"
     CDS             502..732
                     /locus_tag="PK977_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20047.1"
                     /translation="MIDDLTNNFQLKGKNYNEIIELLGQPQGKGENDLQIFYDIDVDY
                     GSDIDPIYTKTLNFQFEKDPIVKTFEVKEWKK"
     gene            854..>1880
                     /locus_tag="PK977_17850"
     CDS             854..>1880
                     /locus_tag="PK977_17850"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HRF20048.1"
                     /translation="MPYSPSPVLQNERTDILDILRGFALLGVMIDNLFGFSGWGFITQ
                     PMRESLATWPADGPLAMLEQVFINGKFYSLFSLLFGIGFSIILIRNEQRGINPLKIFY
                     RRLFILLLIGAGHLFLLWEGDILLLYALIGFVLPLFRKCSDSNLLIIATLLIFSPLLF
                     DAFSILFQYKNGGFLESLAQSIDKKTGLPTDDSFGQYLYKDGAGWQEWRNWQASGWAY
                     RYAYILDSNRIPKVLGMFLIGFWAGRKMIYTQLHNYTSLFKKLRFWGLLIGIPAGIAS
                     FYFEFFQKKIPDPVGIFHTFFYAISVVPLCLAYTSMICLHWLKRKGNTPIKYLAPMGR
                     MALTNYLM"
     assembly_gap    1881..1956
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2111..2587
                     /locus_tag="PK977_17855"
     CDS             2111..2587
                     /locus_tag="PK977_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20049.1"
                     /translation="MKKLTLFFLFALIMFSADAAVIPSENPDAKSIQVALPWSNKTIS
                     LAELATLTPKQYKALTGKKLTLAEKIQLKISQRQARKLIEKDGTVNAEKWKKKFGFFD
                     RWSWHWGGFALGFLVLIGPIICLFINDDYKWDRFWTAMTVTGVIASAAVIFTSSAL"
     gene            complement(2594..>3111)
                     /locus_tag="PK977_17860"
     CDS             complement(2594..>3111)
                     /locus_tag="PK977_17860"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF20050.1"
                     /translation="DFGAVVCKSLSPLCGTCVFKKDCYAFRHNKVNVLPVKEKKISIK
                     KRCFYYLVLEYYKKIAICQRTAKDIWQGLYEFPLIETTSAAEPAGIIKQAVQQKWLKP
                     NGYTIKTLSPLYKQQLSHQHITGQFMIVIMKTKPVITDNWQWIAKDALRQYAFPRMLR
                     HFLHDEMQQNH"
BASE COUNT          825 a          614 c          629 g          930 t
ORIGIN      
        1 ttatggtggg gtggtttatg gcagtttact ttggtttctg gtttcccgtt gcttgttact
       61 cgttgctggt tactcgttac tggttgcttg ttgctggtag cttttgcccg gaagataata
      121 acctaaaact ggtattgcgg aaatgggcag ggctaagtaa tttacaattg agttgcaacg
      181 gtttttaagc aatcgaatgc ttgaattgtc atagtcaagc tggtacaaat gcgcattgat
      241 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg taaatttata tgttgtatgc
      301 aagcctatta gcgacactca tccaaatcaa accattcgta ggacaatatt ttttaggcct
      361 atatctttta attaaatgac aagacaattt atttatttgc ttataacagt tctgctttat
      421 tcttgccagg caaaacaaaa gtttgacaaa atgagatggg cagaagttgc agacttgatg
      481 accttcccaa atagagagtc tatgattgac gacctaacta ataatttcca attgaaaggc
      541 aaaaattata atgaaattat tgaactgctc ggacaaccgc agggaaaagg cgaaaacgat
      601 ttacaaatat tctatgacat agatgttgat tatggaagtg acattgaccc aatttataca
      661 aagacactta attttcaatt cgaaaaagac ccaatagtta aaactttcga ggtaaaagaa
      721 tggaaaaagt aaatcggact acaaagaacg cctgcataca acatgaggtt tgctgctatg
      781 caggctgacg aatatattcc cagctttcac aggtctgctt tctgcaaaat aattatcttt
      841 aaaaaaacca accatgcctt attcaccttc acctgttctc caaaatgaga gaacggatat
      901 ccttgatata cttaggggat ttgccctgct gggtgtaatg atcgataacc tgttcgggtt
      961 tagcggctgg ggttttatta cacagccgat gcgggaatcc ctggccacct ggccggctga
     1021 cgggcctttg gctatgctgg aacaggtatt tatcaacggt aaattttaca gcctgttttc
     1081 actgctgttt ggtataggtt tttccattat actcatccgg aatgagcaac ggggcatcaa
     1141 tccgctgaaa atattttatc gtcgtttatt cattctgctg ctaataggcg ccggccactt
     1201 gtttttatta tgggaaggag atatcctttt actctatgcc ctcatcgggt ttgtgttgcc
     1261 gctttttaga aagtgctcag acagtaattt gctgatcatt gccaccctgc tcatcttttc
     1321 tcccttacta tttgatgctt tcagcatcct ttttcaatat aagaacgggg gcttccttga
     1381 atcgcttgcc caatctatcg ataaaaaaac cggtttgccg accgatgata gtttcgggca
     1441 atacctgtat aaagatggcg ccggctggca ggagtggcgc aactggcagg cttcgggctg
     1501 ggcttaccgt tatgcgtata tattggacag caaccgcatc cctaaagtgc tgggcatgtt
     1561 cctgatcggt ttctgggcgg gccggaaaat gatctataca caattacata attatacttc
     1621 tttgtttaaa aaactacgtt tttggggatt gctcatcggt ataccggccg gtattgcttc
     1681 attttacttt gaattttttc aaaaaaaaat tccggatcct gtgggcatat tccacacttt
     1741 cttttatgcc atcagcgtag tccctttatg cctggcctat acttccatga tctgtttgca
     1801 ctggctcaaa cgaaaaggaa atacacccat taaatatctt gctcccatgg gaagaatggc
     1861 cctcaccaat tacctgatgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattt cgctgggatt agtggtttat
     1981 tttttccaga ttgtttacag caatttatgg ttccggtatt ttaattacgg acccctggaa
     2041 tggatatggc gccagctaac ctatggcaaa cgattacctt taaaaaataa aacaacaact
     2101 ttttaactgt atgaaaaaac tgacactgtt ctttcttttt gcattaatca tgttttcggc
     2161 cgatgctgcg gtaattccat ctgaaaatcc cgatgccaaa agcatacagg tggcgctgcc
     2221 ctggagtaat aaaaccatca gcctggctga actggcaaca cttactccca aacaatacaa
     2281 agcattgaca ggtaaaaaac tgacactggc agaaaagata caactcaaaa taagtcagcg
     2341 acaggcccgt aagctgatcg aaaaagacgg tacagtaaat gcagaaaaat ggaaaaagaa
     2401 attcggtttc ttcgaccgct ggagctggca ctggggtgga tttgcccttg gcttccttgt
     2461 actgatcggt cccatcatct gtcttttcat aaacgatgat tacaaatggg atcgtttctg
     2521 gacggccatg accgtaaccg gggtcatcgc ttcagctgct gtcatattta caagctctgc
     2581 cttatagaaa ttttcaatga ttttgttgca tttcatcatg caggaaatgt cgcagcatcc
     2641 gcgggaaagc atattgacgt aaagcatctt ttgcgatcca ttgccagttg tccgtaatga
     2701 caggtttcgt tttcattatt actatcatga actggccggt gatatgttgg tgagagagct
     2761 gttgcttata cagcggtgaa agggttttta tcgtatagcc attcggcttt agccattttt
     2821 gttgtacagc ctgctttatt atcccggctg gctctgcagc cgatgttgtt tctatcaaag
     2881 gaaattcata cagaccctgc cagatatctt tagcggtgcg ctggcatatg gcaatttttt
     2941 tatagtattc taatacgagg tagtaaaagc agcgtttctt tatgctgatc tttttttctt
     3001 ttacgggcag cacattcact ttgttgtgcc gaaaggcata acaatctttt ttaaacacac
     3061 aagtaccaca aagcggtgaa agggatttgc atactacggc gccaaaatcc a
//
LOCUS       DAORLS010000342         3066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17444_length_3066_cov_1.426769, whole genome shotgun sequence.
ACCESSION   DAORLS010000342 DAORLS010000000
VERSION     DAORLS010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17444_length_3066_cov_1.426769"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>358
                     /locus_tag="PK977_17865"
     CDS             <1..>358
                     /locus_tag="PK977_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRF20051.1"
                     /translation="HSIWIIDRDIVINSITQLFSTHVPATYIADGHHRAASAAKVSKE
                     LPGSENAKYFLTTIFPESQLAILDYNRVVKDLNGLTPEKLLALLQDDFYITLSPEPVK
                     PSHLHEFGMYLEGQWY"
     assembly_gap    359..372
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <373..681
                     /locus_tag="PK977_17870"
     CDS             <373..681
                     /locus_tag="PK977_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRF20052.1"
                     /translation="KGSWTDDPIGVLDVTILSNNILDKIFDIKDQRTDKRIDFVGGIR
                     GLEELEKRVNNGEMKVAFSLYPVTLAQLFAIADSGNVMPPKSTWFEPKLRDGLLTHLL
                     "
     gene            757..>1308
                     /locus_tag="PK977_17875"
     CDS             757..>1308
                     /locus_tag="PK977_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20053.1"
                     /translation="MKKIQLLFIALSAFLLVSAQPEDSKTMHETAKTFMRTGDFDNAI
                     IVLTRALQQDKNNLEMQKDLVMSYYLKRDYAKALDGVKALIDRDDADVVVFQIAGNVY
                     KALEEVKECEKVYKKGLKKFPKSGPLYSEYGELLWAVKDFSAIQQWEKGIEADPAYGG
                     NYYNAALFYFYTKDKVWSLIYGEI"
     assembly_gap    1309..1443
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1444..1779
                     /locus_tag="PK977_17880"
     CDS             <1444..1779
                     /locus_tag="PK977_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20054.1"
                     /translation="AKSFLQVMNKQSSLASKGITTEVLTMIRSRFIIDWYANYAVKYP
                     YRLFDYHRQLLSEGMFDAYNQWLFGSVENLAAYDNWTKTHSEEYNNFSTFQKSRVFRM
                     PPGQYYQVN"
     gene            1906..>2127
                     /locus_tag="PK977_17885"
     CDS             1906..>2127
                     /locus_tag="PK977_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRF20055.1"
                     /translation="MTEPKRLFDAIQLQLENKPLPDMLAGKTGGQWKKFSTAEVAEAV
                     DQLSAGLLSLGISPNDMTVEGRDKVGIISK"
     assembly_gap    2128..2227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2228..>3066
                     /locus_tag="PK977_17890"
     CDS             <2228..>3066
                     /locus_tag="PK977_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF20056.1"
                     /translation="NDAKVKVMIVNDEDAFLKVMSIKERVSSLQYIFSFEHIANCRHW
                     KELLAASTPELISQAKAISEKIKEEDAVTIIYTSGTTGVPKGVMLSHRNILSNVIASS
                     PCFPPGENMRSLSFLPLNHIFERMVTYLYLFNYTSIYYAESLETIGENLKEVQPDMFT
                     TVPRLLEKVYDKIMQKGNELTGTKRKIFFWAHSLAEKFEINKSQGAWYNFQLSLANKL
                     VFSKWREALGNRLRCVVTGGAACQVRLIRIFTAANIPIMEGYGLTETSPVISVNTYDE
                     KGR"
BASE COUNT          876 a          550 c          615 g          776 t
ORIGIN      
        1 agcacagcat atggatcatt gacagggata ttgttataaa ttctatcaca caattgttca
       61 gcactcatgt gcctgctaca tatattgccg atgggcatca ccgggcagct tctgcagcca
      121 aggtaagcaa agaacttcct ggcagtgaaa acgcaaaata ttttcttact acgatcttcc
      181 cggaaagcca gcttgccatt ttggactaca acagggtggt gaaagacctc aatggcctga
      241 cacctgaaaa attacttgca ttgctccagg atgatttcta tataacattg agcccggaac
      301 cggtaaaacc ctctcatttg catgagtttg ggatgtacct ggaaggacaa tggtatatnn
      361 nnnnnnnnnn nnaaaggaag ctggaccgat gaccctatcg gtgtgctgga tgtcactatt
      421 ttgtccaata atattcttga taagattttt gacattaaag accagcgtac tgataaacgg
      481 attgattttg tgggtggcat ccggggatta gaggaattgg aaaaaagagt gaataacgga
      541 gaaatgaaag tagcctttag tctttatccg gttacactgg cacaattatt tgccattgca
      601 gacagcggta atgtaatgcc gccgaaaagc acctggtttg aacccaaact gagagatggg
      661 ctgcttacgc atctgttata agccctttcg ctgaaatatt tgttattttg caacagagac
      721 gtccattata gcgtctcttg ttttttaatt tgcaacatga aaaaaattca gcttcttttt
      781 attgccttat ccgccttcct gctcgtttca gcccagccag aagactccaa aacaatgcat
      841 gaaacggcca aaacgttcat gcggacaggt gacttcgata atgccattat tgtacttacc
      901 cgtgccttgc agcaggataa aaataacctg gaaatgcaaa aagacctggt gatgagttat
      961 tacctgaaaa gggattacgc aaaagccctt gacggagtaa aagccctgat cgaccgggac
     1021 gatgcagatg tggtggtttt tcaaatagct ggcaatgtgt acaaagcatt ggaagaagta
     1081 aaggaatgtg agaaagtata taagaaagga ttaaagaagt ttccgaaaag cggacccttg
     1141 tacagtgaat acggggagtt actttgggct gtaaaagatt tttcagctat tcagcaatgg
     1201 gaaaaaggca ttgaagcaga ccctgcttat gggggaaatt attacaatgc agcacttttt
     1261 tatttctata caaaagataa agtttggagc cttatctatg gtgaaatcnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnngccaaat ctttcctgca ggtaatgaac aaacaaagtt cacttgcatc caaaggcatt
     1501 actacagagg tgctaactat gatccggtcc aggtttatta tagactggta tgccaattat
     1561 gctgtaaaat atccttaccg gttgttcgac tatcatcgcc agcttttatc ggaagggatg
     1621 tttgatgcct ataaccagtg gctttttggc tccgtggaaa accttgctgc gtacgacaac
     1681 tggacaaaaa cccatagtga agaatataat aatttctcta ccttccagaa gagcagggtg
     1741 tttcgtatgc ccccggggca atattaccag gtgaactaat ttttcaaaaa cttgttttat
     1801 cctgttttac aacgtaagac aggatttttt ttatgcgggt ttttgactaa tttgtccatg
     1861 ctaactgctt attctctgat tagttaacga ttcaacgatt gcaccatgac tgaacctaaa
     1921 cgtctatttg atgccataca acttcagctg gaaaacaaac ccctccccga tatgctggct
     1981 ggtaaaaccg gaggccagtg gaaaaaattc agcacggctg aagtggcaga agcagttgac
     2041 cagttaagtg ccggccttct tagccttgga ataagtccta acgatatgac tgtggaaggc
     2101 agggataaag tgggcatcat ttccaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnaaa tgatgcgaag gttaaagtga tgatagtaaa tgatgaagat gcatttctaa
     2281 aagtgatgag cataaaagaa agggtttctt cgttgcagta tattttttcc tttgaacata
     2341 tagccaactg caggcattgg aaagaattac ttgctgcctc aaccccggaa cttatttccc
     2401 aggcaaaagc aatatcagaa aaaattaaag aagaggatgc tgtaaccatt atctatacat
     2461 ctggtacaac cggggtaccc aaaggagtaa tgctgagcca tcgaaacatt ttatcgaatg
     2521 tgattgcttc aagcccctgt ttcccccccg gagaaaatat gcgttcttta agcttccttc
     2581 cgctgaacca tatttttgag agaatggtca catatctcta tctcttcaat tatacttcta
     2641 tctattatgc tgaaagcctg gaaacaatcg gagaaaactt aaaagaggta caaccggata
     2701 tgttcaccac tgtccccagg ttattggaga aagtgtatga taagatcatg caaaaaggaa
     2761 atgaactgac aggtactaaa agaaaaatat ttttctgggc acacagttta gctgaaaaat
     2821 ttgaaatcaa taaaagccag ggagcctggt acaactttca gttgagtctc gccaacaaac
     2881 tggtttttag taaatggagg gaagccctgg gtaaccggtt aagatgtgtg gtaacaggtg
     2941 gcgccgcttg ccaggtacga ctgatcagaa tatttactgc tgccaatata cctattatgg
     3001 aaggttacgg ccttactgaa acctcacctg tgatatccgt aaacacttat gatgagaaag
     3061 gaagaa
//
LOCUS       DAORLS010000343         3064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17458_length_3064_cov_1.928215, whole genome shotgun sequence.
ACCESSION   DAORLS010000343 DAORLS010000000
VERSION     DAORLS010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17458_length_3064_cov_1.928215"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PK977_17895"
     CDS             complement(<1..640)
                     /locus_tag="PK977_17895"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20057.1"
                     /translation="MKGKLYLLSLVVSLLFAVSFTYAQVASKAQVAKQLITSVKETDR
                     PTDTELSAMKLSAETYSRNNGVSHVYFQQHIGEIPVFNAIMGVHITRDNKLLTFNSRF
                     TPLKGSENISANPSLTADQAVIAALNALGLRLATPLVQKQPATGPVRFTVFDKGNVAC
                     EDIKVQLIYQPLEKQGLRLAWQVEIYTAKATDYWVARIDAGTGQLLDKNNLVV"
     gene            complement(918..>3064)
                     /locus_tag="PK977_17900"
     CDS             complement(918..>3064)
                     /locus_tag="PK977_17900"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF20058.1"
                     /translation="LFVSDIEGKFVQRINRGSAERVREVKWMSDNKTILFSQTLDGYV
                     NLYTIRADSIATPKQLINDKRNNRNIVFNKKRTQAVYLSGRDEVRLLDLKTLQSKTIV
                     KDELWAFQNSDPGFSPNDEYVLFTAKRNFEEDIFIHHIKNNKTTNLTNTGVTESGPIW
                     SPDGKYIYFTSQRLKPSYPFGMANAKVYRVALEKLDEPYRIDKYNELFKEEKKDTAKK
                     ANNNTDSLKAITIDMDLLMERLEQISPSLGAQFLQAVYQKGEKTTVLYTSNHGEGRNA
                     LWKTVIEPFEQNKTEKIAGTDNSFGFDIVEVSDKLYVLFNSTLNKLNLDGNKADPVAI
                     SYTFRRNLAEEFNQMFYEAWAGMEEGYYDEKFHGNDWRKTKEYYKQFLSHLNNRADLR
                     TLLNDMLGELNSSHQGFGTFGDDENVPLQNQTMETGILFENDDPYKVKYVVRRSVADR
                     RAVDIKPGDVLVKVNDEQVDKNRDRNYYFTLPSRDRELRLTFNRNGQPINVKIHPQAT
                     LFNNLYDEWIDANQKRVDEKSKGRIAYGYMKNMGQGELEQFIIDMTQELNKKEALIFD
                     LRYNTGGNVHDEVLKFLSQKTYLRWKYREGALTPQSNFGPADKPIVLLINEQSLSDAE
                     MTSQGFKALKLGKIIGNETYRWIIFTSGIGLVDGSSVRMPAWGCYSLEGKDLEITGVQ
                     PDILVINTFDDKLNGRDPQLEKAIDELLKQLK"
BASE COUNT          820 a          691 c          578 g          975 t
ORIGIN      
        1 aaacaaccag gttgttttta tccaataact ggccggtacc tgcatcaatt cttgccaccc
       61 agtaatcagt ggctttggct gtataaattt caacctgcca ggccaaacga aggccctgct
      121 tttcaagcgg ctggtaaatc agctgaacct ttatgtcttc acaggcaaca ttacccttat
      181 cgaaaactgt aaaccgaacg ggacctgttg ccggctgttt ttgtactaaa ggagtagcca
      241 gcctcaatcc caatgcgttt agtgcagcaa taactgcctg gtcggcagtt aaggaagggt
      301 tagccgaaat attttcactg cccttaaggg gtgtaaaccg gctgttaaat gtcagcagct
      361 tattgtccct tgttatatgc acacccataa tagcattaaa cacagggatt tcgccaatat
      421 gttgttggaa ataaacatgc gacactccgt tgttgcggct gtatgtttca gcagaaagtt
      481 tcatagcgga taactcagta tccgttgggc ggtcagtttc ttttacagaa gttatgagct
      541 gttttgccac ctgtgctttg gaagctacct gtgcataggt aaaagaaaca gcaaagagca
      601 gggaaacaac gaggcttaag aggtaaagtt tacctttcat aatgcaggtt ttggttataa
      661 taaaagaatt aaagcaggaa acagattaac tggcttaaac agggtgtgga tgagcaaccc
      721 atcagaagac ttaaccaaat gaagaccgat attacaattt taatttatac tggcaaaaaa
      781 ttattcatga taaaaataat aactgatgat agcttacaat aacagggtct accggttacc
      841 aataaactga aaataaaagg gatactcaaa gagtaccccc tgtattgtga ccccgaagag
      901 attccggtac actggttcta ttttaattgt ttcagtaact catcaatagc cttttctaat
      961 tgaggatccc ttccgtttag tttatcatca aaagtgttga taaccaaaat atccggctgc
     1021 acacccgtta tttcaaggtc tttgccttcc agtgagtaac aaccccatgc gggcatacga
     1081 acggatgaac catctaccag cccgattcca ctggtaaaaa tgatccagcg ataggtttca
     1141 ttaccaatga tcttacccag tttcaatgct ttaaaaccct gcgaggtcat ttccgcatca
     1201 cttaatgatt gctcgttaat cagtaataca atgggtttat cagccggacc aaaattagat
     1261 tgcggggtta aggcgccctc cctgtatttc cagcgcagat aggttttttg agacaagaat
     1321 ttcaatacct catcatgcac attgccaccc gtattgtacc ggagatcgaa tatcagtgcc
     1381 tcctttttat tcagctcctg ggtcatgtca atgatgaact gctccagttc cccttgtccc
     1441 atattcttca tatagccata agcgatcctg cctttgcttt tctcatccac tcttttctgg
     1501 ttggcatcaa tccattcatc gtacaaatta ttaaataaag tagcctgcgg atgaatcttc
     1561 acattgattg gctgtccatt gcgattaaat gttaaacgca gctccctatc ccgggatggc
     1621 aaagtgaaat agtagtttct gtccctgttt ttatctacct gttcatcatt tactttcacc
     1681 agcacatcgc cgggttttat atcaactgcc cttctgtctg ctactgaccg acggaccaca
     1741 tatttcactt tataaggatc atcattttca aacaggatac ctgtttccat ggtttgattt
     1801 tgcaacggaa cattttcgtc atcgccaaat gtgccaaaac cctggtgcga agaatttagc
     1861 tcacccagca tatcattcag caaggttctc aggtctgcac ggttattgag atgggagaga
     1921 aattgtttgt aatactcttt agtttttctc cagtcgttgc catggaattt ttcatcataa
     1981 taaccttctt ccatccctgc ccatgcttca tagaacatct ggttaaattc ttcagccagg
     2041 tttcttctga aggtgtagct gatggcaacc ggatcagctt tgttaccatc caaattcagc
     2101 ttatttagtg tactgttgaa caagacatac aacttatcac tcacttccac aatatcaaac
     2161 ccaaaactat tatctgtacc tgctatcttt tctgttttgt tttgttcaaa tggttcaatg
     2221 actgttttcc agagtgcatt ccggccttca ccatggttgc tggtgtataa caccgttgtc
     2281 ttttctcctt tctggtaaac tgcctgtaga aattgagccc ccagtgaagg actaatctgc
     2341 tccaggcgtt ccatcagcag gtccatatct attgtaattg ccttaagaga gtcggtatta
     2401 ttatttgctt tcttagcggt atccttcttc tcttccttaa acagctcatt gtatttatct
     2461 atccggtaag gctcatccag tttttccaaa gccacccggt acacttttgc attggccatg
     2521 ccaaatggat aggaaggctt cagtcgctgt gaagtgaagt aaatatattt tccatcaggt
     2581 gaccagatgg ggccgctttc cgttacgccg gtattggtaa gattggtggt tttattgttt
     2641 ttaatatgat gaatgaaaat atcttcttca aagtttcttt tggcggtgaa caacacatat
     2701 tcatcattgg gagagaagcc tggatcacta ttctgaaaag cccacaactc atcttttaca
     2761 atggttttgc tttgcagtgt cttcaggtca agcaatctta cttcatccct gccactcagg
     2821 taaacagcct gtgtcctttt tttattgaaa acaatattcc ggttatttcg cttgtcgttt
     2881 atcaactgtt ttggcgtagc aatactgtcg gcccttatcg tatatagatt gacatatcca
     2941 tccagtgttt ggctgaaaag gattgtttta ttatcactca tccattttac ttctcttact
     3001 ctctctgcac tgccccggtt gatccgctgc acaaattttc cttcaatgtc gcttacaaaa
     3061 agtt
//
LOCUS       DAORLS010000344         3057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17526_length_3057_cov_1.386076, whole genome shotgun sequence.
ACCESSION   DAORLS010000344 DAORLS010000000
VERSION     DAORLS010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17526_length_3057_cov_1.386076"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PK977_17905"
     CDS             <1..272
                     /locus_tag="PK977_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRF20059.1"
                     /translation="HGGASCVLAETIGSVSSDMVVDHSKYYCVGLEINANHIRSAKEG
                     FVSGTATPLHLGATTHVWDIKIHDDKHKLICVSRLTVAVIKRKES"
     gene            complement(274..>581)
                     /locus_tag="PK977_17910"
     CDS             complement(274..>581)
                     /locus_tag="PK977_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF20060.1"
                     /translation="KLDKGEKLEDCAIREIEEETGLKEVKLIKPLCVTYHTYHEGARF
                     ILKDSHWYIMKVKGYQQLVPQTEEDIHEIRWVKKTGLAEYMKISYPSVADVLRSVAE"
     assembly_gap    582..638
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<639..894)
                     /locus_tag="PK977_17915"
     CDS             complement(<639..894)
                     /locus_tag="PK977_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF20061.1"
                     /translation="MYIKVYFDDKPLFLCDKIDEVIEPFIHHDDAIFIDELNSHTIKT
                     IIHEMQQSQVHAGVFFHSDLDALKQAFFKKFTLVTAAGGLV"
     gene            887..>1461
                     /gene="pyrE"
                     /locus_tag="PK977_17920"
     CDS             887..>1461
                     /gene="pyrE"
                     /locus_tag="PK977_17920"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRF20062.1"
                     /translation="MYITKFSYFVPAVDFCAMTDAKVVAEKLLQVNAVKLNTQQPWAS
                     GWKSPIYCDNRKVLSFPFTRDYIKSELCNVIFEKFPEAELLAGVATAGIAWGAMAADQ
                     LKLPYVYVRPKPKEHGLGNQIEGFYEQGQKTVVIEDLVSTGKSSLQVVDVLKEQGLEV
                     IGMVSIFTYRFPAATEAFLKASLTYYPLTDYP"
     assembly_gap    1462..1590
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1603..2001
                     /locus_tag="PK977_17925"
     CDS             1603..2001
                     /locus_tag="PK977_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRF20063.1"
                     /translation="MKKFLITAITVIGFTFTGLAQTKAVLTATIKTPNALCENCKKRI
                     EGYVKPYDGVLEITVNYRKAETRVKYITDRINIEEIKAYIANCGYDADDVPAAEDAYK
                     RLPKSCKKFDDGGGHPKPKPVPAAPAGGQG"
     gene            complement(2013..2510)
                     /locus_tag="PK977_17930"
     CDS             complement(2013..2510)
                     /locus_tag="PK977_17930"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRF20064.1"
                     /translation="MNRFQYRLIASLPLQIAFVLASCGSIKEPDLRGIENVRVDKINS
                     ATSTMRLDLRYFNPNKFRVKLKNASGDAWLEGKKLGSFSLDSAVNVSPKSDFTLPVTL
                     EVDMKRVLSNTLTLLLSNEVTLKIDGTARIGKSGITINYPLKYEGKQKLSELLGQSST
                     NVIQK"
BASE COUNT          874 a          528 c          581 g          888 t
ORIGIN      
        1 tgcatggagg tgcttcttgt gtactggcag agacgattgg aagtgtgtca tctgacatgg
       61 tagttgatca ttcaaaatat tattgcgtag ggcttgagat caatgccaac cacattcgca
      121 gtgcaaaaga agggtttgtt tcgggaacag ccacaccgct tcatttaggc gctactaccc
      181 atgtgtggga tattaagatc cacgacgata agcataaact catttgcgta agcaggttaa
      241 ctgttgcagt aataaagaga aaggaaagct gatttattct gcgaccgatc gtaagacatc
      301 agctactgaa gggtaggaga ttttcatgta ttctgcaagt cctgtttttt ttacccatct
      361 gatttcatgg atatcctctt cggtttgtgg caccagttgc tgataacctt ttactttcat
      421 aatataccag tgagaatctt tgagaataaa ccgggctcct tcatgataag tgtgataagt
      481 aacgcagagt ggcttgatta attttacttc tttcagaccg gtttcctcct ctatctctct
      541 gatagcacaa tcctccaatt tttcaccctt atccagtttt cnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt accaatccgc cggctgctgt
      661 taccaaggtg aattttttaa agaaagcttg tttcaacgca tctaaatcag aatgaaaaaa
      721 aacgccggca tgaacctgtg attgctgcat ttcatggatg atggttttaa ttgtatgact
      781 attcagttca tcaataaaaa tggcatcatc atgatgaata aagggctcta ttacttcatc
      841 aattttatca cagaggaata atggtttgtc atcgaagtat acttttatgt acataactaa
      901 attttcttat ttcgttccgg cagttgactt ttgtgccatg acagacgcaa aagttgttgc
      961 cgaaaagctg cttcaagtta atgccgttaa attaaatact caacagccat gggccagtgg
     1021 atggaaaagc cctatttatt gtgataacag gaaagtactc tcatttccct ttacaagaga
     1081 ttatatcaaa tcagagcttt gtaatgtaat ttttgagaaa tttccggagg cagaattgct
     1141 tgccggtgta gccaccgcag gtattgcctg gggagcgatg gcggctgacc agttgaaact
     1201 accctatgtg tatgtacggc caaaaccaaa ggagcatggt ttgggtaacc agatagaagg
     1261 tttttatgaa cagggacaaa agactgtagt tattgaggac ctagtatcaa ccgggaaaag
     1321 cagcctgcag gtggtagatg tattaaagga acagggtctg gaagtaattg gcatggtatc
     1381 catattcact tatcgctttc ctgctgcaac tgaagccttt cttaaagcat cattgactta
     1441 ttacccctta acagattatc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taattttaac gtatgaagaa gttccttatt
     1621 actgctatca ctgtcattgg atttacattt acgggtttgg cccaaacaaa ggctgttctt
     1681 acggcaacca tcaaaacccc taatgccctt tgtgaaaatt gcaagaaaag aattgagggc
     1741 tatgttaagc cctatgacgg ggtgctggaa ataacggtca attaccgcaa ggccgaaaca
     1801 agagtgaaat atatcaccga ccgtattaat atagaggaaa ttaaggccta tatcgctaac
     1861 tgcgggtatg atgcagatga tgtgcctgca gctgaggatg cttacaaaag actgcctaag
     1921 tcctgcaaaa aatttgatga tggtggcggc caccccaagc ctaaacctgt tccagctgca
     1981 cctgccggag gacaaggtta atttgatcta atttatttct gaataacatt agtcgatgac
     2041 tgacccaaca attctgacag cttttgttta ccctcatatt taagcggata gttaatagta
     2101 attccgcttt tgccaatacg ggctgtgcca tcaattttta gtgttacctc gttacttaaa
     2161 agaagggtga gggtgttgct aagcactctt ttcatatcta cctccagtgt aaccggcagt
     2221 gtaaaatcgg atttagggga aacattcaca gctgaatcca aagagaagct tcccagcttt
     2281 tttccctcca gccaggcatc accacttgca tttttaagct tcacacgaaa cttatttgga
     2341 ttaaaatatc gaaggtccag ccgcatggtg gacgtcgctg aattgatttt atccactctc
     2401 acattttcta tcccccttag atcaggttct tttatcgacc cgcaggaagc taaaacgaaa
     2461 gcaatctgga ggggtaggga ggcgataagc cggtactgaa atctgttcat aacagggttc
     2521 aaattataaa aatactttca gttaaaagta agcaggggcg tcaatgtatt gataattttt
     2581 tgactaataa tattggaaaa gaagtgaaac ttcaaagtta aagcatatac caatattatt
     2641 agtttttacc aaattcttaa atatctaata tacaattata tatgagtatc agtatgtatt
     2701 tataatgtat aaaaattgtc gtatttgata tctaataggc gtatcaaata attaacttaa
     2761 acagaggtgt aaaaaattat ataattggtt catatgtaat atttaatgag ttttgtcggc
     2821 ataaatattt tcaataatcg atttgattca ataatttttt aatttgattt aatcttcttt
     2881 gggacaattc cctttttgag tgaacatttt tttgaggctt taagtgtata ggctcgttta
     2941 cttccggttg caaaaagtcc ggaaaaagag gactgtagag ttgaagaaat acaatcaggt
     3001 tgctagtttt ccagaaccat aaaacaggat acaaaatatc agatagtttt tgctaaa
//
LOCUS       DAORLS010000345         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17555_length_3052_cov_1.965632, whole genome shotgun sequence.
ACCESSION   DAORLS010000345 DAORLS010000000
VERSION     DAORLS010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17555_length_3052_cov_1.965632"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>260)
                     /locus_tag="PK977_17935"
     CDS             complement(<1..>260)
                     /locus_tag="PK977_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20065.1"
                     /translation="EPAKEHRLDNKQPAHGAFTLALMEVLKQQSAKTSVQNIFNSVRA
                     MIKAFGKTQEPVLAGDPSRFNETLLGIEKGLLSDKIYFPVLD"
     assembly_gap    261..358
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<359..944)
                     /locus_tag="PK977_17940"
     CDS             complement(<359..944)
                     /locus_tag="PK977_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF20066.1"
                     /translation="MKKTFTLLTALLANFTLLIAQPAQEPVKKALLIGIDTYNPPKGV
                     KVSNASGRLEWPDLSGCKNDALAMKDIITTKFGFNAKDISELYDAKATRAAILKGMED
                     LLANTPENGVALIYYAGHGSYITNSLSAEGDGYDESMVPADTWKEGVPDIRDKEIARM
                     FNRFVDKNIKLTAIFDCCHSGSIARGILPDPGKSR"
     gene            1255..>3052
                     /locus_tag="PK977_17945"
     CDS             1255..>3052
                     /locus_tag="PK977_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF20067.1"
                     /translation="MALYKNIILTGLFLLLSILSFAEPAGVSQEQTLVLAANQYPQQL
                     KKHRPDQVYILFDGTPYMKKYEGLFLKKDTITNLKRFTDSLGYGIMGYRLIFGKGEKV
                     DSVLQFNTEFRLTDKAVNLGMNKLRLFIFNKKGDPTDQFVLDYSNKLNKEVKGLDELF
                     RKYTVYTYIDKEKYSVRNVWILSIGIDDYGGDVFRYCESDATAYDAFFAKQFLNYQGY
                     FKDEQIKRFLLLGNHATRDSILSVLNTIASQASVNDYFIFNFSGRTWLFDESFKAFSV
                     NKDSSKFTTYFFPYNVIYDDEIKNRDTADTTTPSKNLISLKTLQEYIQLIPCVNQLFI
                     SEAGPSEKFKTEFVKTMMQNSPEIASLLNINRVIIVPNRFGLDELKGTGMGPIAYYLT
                     HLDSTKNIYNIFSNDHRSAEIAYLLKSEEFKLPPGENNDYFSVFFERAFLQQYREIFG
                     DAKVKKRGLDLGGADINTIKSFSGNRHALIIGTDNYRSKDWEKLENPVYDATEVAEVL
                     QSFYNYDVTLLKDPPMDTIYKYLSTYYRSLREDDQFIIYIAGHGDFDEGLLDDGFIVC
                     TDSKSPEVDPLRNTYIQHTKLKKMINKIPAKQV"
BASE COUNT          886 a          544 c          592 g          932 t
ORIGIN      
        1 atctaaaacc ggaaagtata ttttatcaga cagaagccct ttttcaatgc cgagcagagt
       61 ttcattgaag cgggaaggat cacctgccaa aacaggttcc tgggttttgc caaaggcctt
      121 gatcatggcc ctaacactat tgaaaatatt ctgaacggag gtttttgcag attgctgttt
      181 caaaacttcc attaatgcca gggtaaaggc cccgtgggct ggctgtttat tatcaagccg
      241 atgttctttg gcaggttcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
      361 cttgatttgc ccgggtctgg taaaatgcct ctggcgatag agccgctatg gcagcaatcg
      421 aagatggcag ttagtttaat gttcttatca acaaagcgat tgaacatcct ggctatttcc
      481 ttgtcacgta tatccggaac accttctttc caggtatctg caggaaccat cgattcgtca
      541 taaccgtcac cttctgcact caatgagttg gtaatatagc ttccatgacc tgcataatag
      601 atcagcgcaa cgccattttc gggggtgttt gccagcaaat cttccatgcc tttaagaata
      661 gcagcccttg tggctttggc gtcgtataat tcagagatgt cttttgcatt gaacccaaac
      721 tttgtcgtaa taatgtcttt cattgccagg gcatcatttt tacagccact caggtcaggc
      781 cattccagcc ggccagatgc gttagatacc ttgacacctt tgggaggatt ataagtgtca
      841 ataccaatta ataaagcctt tttaaccggc tcttgtgcag gctgggcaat aagcaatgtg
      901 aaattggcaa gcagcgctgt taataaggtg aaagtcttct tcataactgg ttttttgtcg
      961 ttcggttgat taggatattc aaactgacat ccgggtatta ttattggttg caaattgtca
     1021 tctatattac ttattttttt tcagtcggcc atttttgctt tccctggtca ctttgccttt
     1081 ctggctaagg ttacgtaaaa atagcccatg atagattgaa aaatctaccc tgttatgggt
     1141 atatttttcg gaaaaggggc aattggcatg gtatgaatat tccttttttg ataaatacca
     1201 gccgcacggt atggccggct tttatagtag ctttatcttt tgaaaaattt tgccatggcg
     1261 ctgtataaaa atattattct taccggccta tttcttctat tatcaatttt gtcctttgct
     1321 gaaccggctg gtgtctcaca agaacaaact ttggtattgg cagctaacca atatccccaa
     1381 caattaaaaa aacatagacc ggatcaggta tatattcttt ttgacggaac tccttatatg
     1441 aaaaaatatg aaggtttgtt cctgaagaaa gataccatta ccaacctgaa acgttttaca
     1501 gattcccttg gttatggtat tatgggttat cgtctgattt ttgggaaggg ggaaaaggta
     1561 gacagtgttt tacagtttaa tactgaattc aggctaacgg ataaagcagt taacctgggg
     1621 atgaataaac tccggttgtt tatttttaat aaaaagggtg acccaacaga ccagtttgtt
     1681 ctggattaca gcaataaact aaataaagaa gtgaaggggc ttgatgaatt attcagaaag
     1741 tatactgttt atacctatat agacaaagaa aagtatagtg tgcgaaatgt ctggatttta
     1801 tcaattggta ttgatgatta cgggggtgat gtattccggt attgtgagtc agatgctact
     1861 gcttatgatg cttttttcgc caaacagttt ttgaactatc agggctattt taaggatgaa
     1921 caaatcaaaa gatttctttt acttggcaat catgcaacaa gagattctat tctttccgtt
     1981 ttgaatacaa tagcatcgca ggcatcagtt aatgattatt ttatttttaa tttcagtggc
     2041 agaacctggt tgtttgacga aagttttaaa gcattttcag tcaataagga ttcgtcaaaa
     2101 ttcactactt atttctttcc ttataatgtt atttatgatg atgaaattaa aaaccgtgat
     2161 accgcagata ccactacacc ttctaaaaat ttaatctcac ttaaaacact gcaggaatac
     2221 atacaattga tcccctgtgt caaccagtta tttatatcag aggccggtcc atctgaaaaa
     2281 ttcaaaacag agtttgtgaa aacaatgatg cagaactctc ctgagatagc ctcgctgctg
     2341 aatattaatc gtgtaattat agtacctaat cggtttggac tggatgaact taaaggaact
     2401 ggaatgggac ctattgcata ttaccttact cacctggatt caacaaagaa catttacaat
     2461 attttcagca atgatcatag aagtgcagaa atcgcttatt tgttaaagtc cgaagaattt
     2521 aaactacctc cgggtgaaaa caatgattac ttctccgttt tctttgagag agcattcttg
     2581 cagcaatacc gggaaatttt cggtgatgca aaagttaaaa aaagaggtct tgacctggga
     2641 ggggcagata ttaataccat taaatctttc tctggtaaca ggcatgcctt aataattggt
     2701 acagataatt acaggtcaaa agactgggag aaactggaaa atccggttta tgatgccaca
     2761 gaagtagcag aagtattgca aagcttttac aactacgatg tgacactact aaaagatccc
     2821 ccgatggata ctatttacaa gtatctcagt acttattatc gtagccttag ggaagatgac
     2881 caatttatta tttacatagc cggtcatggt gattttgatg aaggtctgct ggatgatgga
     2941 tttatcgttt gtacagattc aaaatcaccg gaagttgacc cactgcgtaa tacttatata
     3001 caacatacca agctgaagaa aatgatcaat aaaatcccgg caaagcaggt at
//
LOCUS       DAORLS010000346         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17556_length_3052_cov_1.927261, whole genome shotgun sequence.
ACCESSION   DAORLS010000346 DAORLS010000000
VERSION     DAORLS010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17556_length_3052_cov_1.927261"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK977_17950"
     CDS             complement(<1..310)
                     /locus_tag="PK977_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20068.1"
                     /translation="MQNFTKKANTLLIFFLYSTSIFAQLPCNNWLNTPSEPSYIRIGD
                     LDIAGTHITVEVNFNRTAPENPAGGYGFLVSKHTGPANTNYALWPNGCAITTTGSGHH
                     L"
     assembly_gap    332..362
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<363..>632)
                     /locus_tag="PK977_17955"
     CDS             complement(<363..>632)
                     /locus_tag="PK977_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRF20069.1"
                     /translation="LIAFRSIEKGLGKLVINDAKIYEDLENNWAVVAEAIQTILRREN
                     YPNPYEALKDLTRGNNKIDKKAIHQFIDKLKIKDEIKRELKKISP"
     assembly_gap    633..725
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<726..1674)
                     /gene="purB"
                     /locus_tag="PK977_17960"
     CDS             complement(<726..1674)
                     /gene="purB"
                     /locus_tag="PK977_17960"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRF20070.1"
                     /translation="MELTNLTAISAVDGRYRKQVQHLDEYFSEYGLMKYRVMVEVEYL
                     LFLEKKKIFKLPGKTTKHLQKLLEDFGPEDAQEIKQIEFTTNHDVKAVEYFLKNELEK
                     CGGQAAKEWVHFGLTSQDINNTAIPLSWKHAIEYEYLPALLNLNTELKLLAEEWKDIP
                     MLAHTHGQPASPTRLGKEIMVFVERIQNQIEQFIGIPFTGKFGGATGNFNAHHVAFPK
                     TDWIKFGNDFVRHLGLTRQQFTTQIEHYDNLAAHFDCMKRINNILTDFCRDTWSYVSM
                     EYFRQKTKKGEVGSSAMPHKVNPIDFENAEGNLGIANSLF"
     gene            1732..2478
                     /locus_tag="PK977_17965"
     CDS             1732..2478
                     /locus_tag="PK977_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HRF20071.1"
                     /translation="MEKIRVGIVNYLNTKPLIYGLDKPPVANLIELVGDYPSRVAELL
                     IKGEVDIGLIPVAVIPQLPSAHVVGNYCIGTEGEIASVALFSEVPMYEIKKVYLDYQS
                     RTSVALLKYLMKEYWGINPEIVEAVDEDYRKEIKGTTAGLVIGDRALEQRKISTFIYD
                     LGSEWRKITGLPFVFAAWVSLKPLPQDFITLFDKANAEGLLHIDEIVLNTPYELYDLK
                     KYYQLHLSYDLDDAKQRGMNHFLSVIKEIE"
     gene            complement(2487..>2684)
                     /locus_tag="PK977_17970"
     CDS             complement(2487..>2684)
                     /locus_tag="PK977_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20072.1"
                     /translation="IEYHGLYDQQEELTQIFQILTRAGFNYYIKEAQAIYEHPFSKVK
                     DPAIPYQIQLNIFCFRKQQVV"
     assembly_gap    2685..2701
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2702..>3052)
                     /locus_tag="PK977_17975"
     CDS             complement(<2702..>3052)
                     /locus_tag="PK977_17975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRF20073.1"
                     /translation="SVIYLKELYPDARITAFEPDEKNFELLSKNVAARSYKNIELRKE
                     AIWIANTEIEFSEDASMSSRIEESGVTGVRKVKAIRLKDLLTDKVDFLKIDIEGAEFK
                     VLSDIEEKLNVINN"
BASE COUNT          791 a          561 c          581 g          978 t
ORIGIN      
        1 tcaggtggtg tccgctaccg gtagtggtaa tagcacaacc atttggccag agagcataat
       61 tggtatttgc aggacctgta tgtttagaaa caaggaaccc gtatcctcct gcaggattct
      121 ccggtgctgt tctgttaaaa ttgacttcaa ctgtaatgtg tgtgccagcg atatccagat
      181 cacctattct gatatatgaa ggctctgatg gagtattaag ccaattattg caagggagtt
      241 gtgcaaaaat tgatgtggaa taaagaaaaa agatcaataa cgtatttgcc ttcttcgtaa
      301 aattctgcat gttttagcta ttacttgaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nntgcggact tattttctta agctccctct ttatttcatc ttttattttg agtttatcta
      421 tgaactggtg aatggctttt ttatcaatct tattatttcc ccttgtcaga tcctttaagg
      481 cttcatatgg gttaggataa ttttctctac gtagaattgt ttgtatggct tctgctacaa
      541 ccgcccagtt attttcgagg tcttcataaa tctttgcatc attaataacc aatttgccta
      601 atcctttttc aattgacctg aaagcgatta atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnncaaat aatgaattgg caattcccag attgccttct gcattttcga aatcaatcgg
      781 gtttactttg tgaggcatgg cagaggagcc tacttctccc ttttttgtct tttgacggaa
      841 atattccatt gatacataac tccatgtatc ccggcaaaaa tcagtaagga tattgttgat
      901 ccttttcata caatcaaaat gagctgccag gttatcataa tgctcgatct gtgttgtaaa
      961 ctgctgccgg gtgagaccta aatgccttac aaaatcattt ccaaatttta tccagtctgt
     1021 tttcgggaat gcaacatgat gagcattaaa gttacctgtc gcccccccaa acttaccggt
     1081 aaaaggaatt ccgataaatt gctcaatctg gttttgtatc cgctccacga ataccattat
     1141 ttctttgccc agcctggtgg gactggcagg ttgtccgtgt gtatgtgcca acataggaat
     1201 gtctttccat tcttctgcca gcaatttcaa ttctgtatta aggttaagca aggctggcag
     1261 gtactcatat tcaatggcat gcttccatga aagcggaata gcagtgttat ttatgtcctg
     1321 tgaggtaagc ccgaaatgta cccattcttt ggctgcctgt cccccgcatt tttccagctc
     1381 atttttaaga aaatattcaa ctgccttaac atcatgattg gtagtaaatt caatttgttt
     1441 aatttcctgg gcatcttccg ggccaaaatc ttccagcagt ttttgaaggt gtttagtggt
     1501 tttgcccgga agtttaaaga tttttttctt ttccaggaat aacaggtact ccacctccac
     1561 cattacccgg tatttcatta agccgtattc tgagaagtat tcatccaaat gctgtacttg
     1621 tttgcgataa cggccgtcaa cggctgaaat agcggttaga ttcgtaagtt ccatgatatt
     1681 tttattaaag aaggctgacc ctttaccttt gcagccacaa aagtaattta attggagaag
     1741 attcgtgttg gcatagttaa ctaccttaat acaaagccat taatctatgg gttagataaa
     1801 cccccggttg ccaaccttat tgaacttgta ggtgattatc cctccagggt tgcagagttg
     1861 ctaataaaag gagaagtgga tattggtctg ataccggttg cggtaatacc ccagctgcct
     1921 tcagcacatg tggtaggtaa ttattgtatc ggaaccgaag gcgaaattgc ttctgttgcg
     1981 cttttcagtg aggtaccaat gtacgaaata aaaaaggttt atctcgatta ccaaagccgt
     2041 acttcagtgg ccctgctaaa atatctaatg aaagaatact ggggtatcaa tcctgaaatc
     2101 gtcgaggcgg tagatgaaga ttatagaaag gaaattaaag gcactactgc cgggttggtt
     2161 ataggtgaca gggcacttga acagcgaaag atttctactt ttatttacga tctgggctct
     2221 gaatggagga aaataacagg gttgcctttt gtttttgcag cgtgggttag tttaaagcca
     2281 ttaccgcagg atttcattac cctgtttgac aaggctaatg ctgaagggct tctgcatatt
     2341 gatgaaatcg tgttgaatac tccttatgag ttatatgacc tgaaaaaata ttaccaattg
     2401 catttaagct atgatctgga cgatgcaaaa cagagaggaa tgaatcattt tctcagcgta
     2461 attaaggaga tagaatagga attaatttaa actacttgct gtttcctgaa gcagaaaata
     2521 tttaattgta tctgataggg gattgccggg tcttttactt tggaaaatgg atgttcgtaa
     2581 atggcctgtg cttccttaat ataataattg aaaccagccc tggtcagtat ctggaaaatc
     2641 tgagtcagtt cttcctgttg atcgtagagg ccatggtatt caatnnnnnn nnnnnnnnnn
     2701 natattattt ataacgttca gtttttcttc gatgtcgctc aatactttga attcggctcc
     2761 ttcaatatcg attttgagaa agtcaacttt atctgtcagc aggtctttta atcttattgc
     2821 tttcactttt cttacgccag ttactccgga ttcttcaatt cggcttgaca ttgacgcatc
     2881 ttccgagaac tcaatttcag tgttggcaat ccatatagcc tcttttctta attcaatgtt
     2941 tttataactc cttgcagcaa cgttttttga taatagctca aaattttttt cgtcgggctc
     3001 aaatgcggtt atccgggcat ccgggtatag ttctttcaga taaattacac tc
//
LOCUS       DAORLS010000347         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17584_length_3049_cov_1.965598, whole genome shotgun sequence.
ACCESSION   DAORLS010000347 DAORLS010000000
VERSION     DAORLS010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17584_length_3049_cov_1.965598"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..627)
                     /locus_tag="PK977_17980"
     CDS             complement(43..627)
                     /locus_tag="PK977_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20074.1"
                     /translation="MKYRSLLLLAISYLVAGCNPKEKEPDKKYVSVVSLIKKQVEHVD
                     TSLYPIIKITTVDSLPPDTIYIPREEFVTVAKDFLDIPDLSDPKVAKKYKEEQARYDE
                     SLNRVVITYTPLNPDKEEVKKQELLVTPSEAAGDKVSNVIVLREINNRDSFFQKKMLW
                     QMDKSFQVVTTLQKPGTPEKITILKVSWNEPKEE"
     gene            complement(744..2201)
                     /locus_tag="PK977_17985"
     CDS             complement(744..2201)
                     /locus_tag="PK977_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20075.1"
                     /translation="MDRRYNNRDFEHFLKQNADQYRMFPSEKVWEGIHHTLHTRRRWY
                     GIGLALLLLSTATVTTVMLNNAGKKQQLADKAPVTKTIDILSKQAASNTVIIAPQKPA
                     ANNSGSQRPDKLQASLVADQEQLIAAVPELEEQAETVTLEAIPEINLKSAVANIQRSS
                     LQKNTEILKKSAPALPTKKALVKPSPPVASLQPENNTIIVDNTVEKKEIKAEAENSIA
                     TERKENSPYTIESVVNSYKYKKSRKKLSWQIYITPTISYRKLKENRTFLEAARTTLGT
                     PTNYTYSFSDINSIVTHKPDIGLQLGVVTGYPVSKRLRIIAGLQFNVSKYDIRAYNHD
                     GEVATIALTNNAGGTNTVSTITNYRNIGGYKADWLHNMYISGSAPIGLELKLSGNKKT
                     SVGIAGTIQPTYVLGNRAYLLSADFKNYAEVPSLTRKWNLNTGFEVYAGYSTGKLDWR
                     IGPNVRYQVFSSFIDKYPIKEHLFDFGLKLGLMLK"
     gene            complement(2237..2818)
                     /locus_tag="PK977_17990"
     CDS             complement(2237..2818)
                     /locus_tag="PK977_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF20076.1"
                     /translation="MTEEAIIKGCLKNDATAQRELYNRYSPKMLAVCYRFAQNREDAE
                     DMLQEGFIKVFLQIHTFENRGAFEGWIRRIVVHTCINILKKNKKFNESVDIIHATGVQ
                     IREESVPAIIQAKQVVECIRLLPIGYRTVLNLYAVEGYSHREIANMLDIEESTSRSQY
                     TRAKAMLEDILIRKKILSKPRPEADWLAAVSQR"
BASE COUNT          769 a          613 c          642 g         1025 t
ORIGIN      
        1 ctttcggtat agtaggagcg atatgttcaa attcctgctg tgtcattctt ctttaggctc
       61 attccagctt actttcagta tagttatttt ttcaggcgtt cccggttttt gcagtgttgt
      121 taccacctgg aagctcttat ccatttgcca gagcattttt ttttgaaaga agctatccct
      181 gttgttgatc tcccttagta caatgacatt gcttacttta tctccggctg cttcgcttgg
      241 ggtaaccagt aattcctgtt tttttacctc ttccttatcc gggtttagcg gggtataggt
      301 gattacaacc ctattgagtg attcatcata ccgtgcctgt tcttccttgt acttttttgc
      361 tactttggga tcagacagat cgggtatatc caaaaaatct ttagccacag tgacaaactc
      421 ttctctcggg atatagatgg tatccggagg caaactatca accgttgtta ttttaataat
      481 cgggtataac gaagtgtcca catgttctac ttgttttttg atcagggaga caactgaaac
      541 atatttcttg tcaggttctt tttcttttgg gttacaacct gcaactagat agctaattgc
      601 cagcagaagt agtgacctgt atttcatctt tcaaaattaa ctgaaacagc atttatgaga
      661 tgatcaaaag gaatgtaaaa cgtaaaataa aatcccgcac ctatggaacg ggattttacc
      721 aagcagggca gttagtgctg gttttatttg agcataaggc ccaattttaa accaaaatca
      781 aaaaggtgtt ctttgatggg atatttatca ataaagctgg aaaatacctg gtaacgaaca
      841 ttgggaccaa ttctccaatc cagtttgccg gttgaatacc cggcataaac ttcaaacccg
      901 gtattcaggt tccattttcg ggtaagcgaa ggaacttctg catagttttt aaaatcggca
      961 gatagcaaat aagccctgtt cccgagaaca taagtaggct ggatggtgcc ggctatgccc
     1021 acagatgttt tcttattccc tgaaagttta agttcaaggc caattggggc agatcctgaa
     1081 atatacatgt tgtgcagcca gtcagcttta tatccaccaa tattgcgata gttggtgata
     1141 gtagaaaccg tgtttgtacc accggcatta ttggtgagtg caatagtggc tacttctccg
     1201 tcatggttgt aggcccttat atcatatttg ctaacattga actgaagccc ggcgattatc
     1261 cgtaatcttt ttgaaacagg gtaaccggtt actactccta attgcaggcc gatatccggt
     1321 ttgtgggtaa caatgctgtt gatatcagag aatgaataag tatagtttgt tggggtgccc
     1381 agtgtagttc ttgcagcttc cagaaaagtt ctgttttctt tcagcttacg ataagatatg
     1441 gttggtgtta tgtaaatctg ccaagagagc tttttacggg attttttata tttatatgaa
     1501 ttgactacgc tttcgattgt atagggactg ttttcttttc tttcagttgc tatactgttt
     1561 tcagcttcag ccttaatttc tttcttttca acggtattat caactatgat agtgttgttt
     1621 tccggctgta atgatgctac cggcggactg ggttttacca gtgccttctt tgttggtaaa
     1681 gctggtgccg atttcttcaa tatttctgta tttttttgaa gagatgatcg ctgaatatta
     1741 gctacggcgg attttagatt gatttctgga atggcttcca gggtcactgt ttcggcctgt
     1801 tcttcaagtt ctggcacagc ggcaattaat tgctcctgat cagccacgag gctggcttgt
     1861 aatttatcag gtctttggct tccactattg tttgcagcag gtttctgcgg ggcaatgatt
     1921 acagtatttg aagcagcctg tttagaaaga atatcgattg tttttgttac aggagcctta
     1981 tctgccaatt gttgtttttt gcctgcattg ttgagcataa ccgtagtaac tgttgcagtt
     2041 gaaagaagca ataaggccag accaattccg taccagcgac gacgggtatg aagggtatga
     2101 tggatgcctt cccaaacttt ttctgatgga aacatcctgt attggtcagc attttgtttt
     2161 agaaaatgct caaaatctct gttgttatac ctcctatcca taaccagtca gtagtttagg
     2221 gtacgtttaa atctttttat ctctggctaa cggcggcaag ccaatcagct tcaggtctgg
     2281 gttttgatag tattttttta cggatcaaaa tatcttccag catcgccttt gccctggtgt
     2341 actggctccg gctggtgctt tcttcaatat ccagcatatt ggctatttcc cgatggctat
     2401 aaccttccac agcatagagg ttaagaactg tccggtagcc gatcggcagt aaccttatac
     2461 attctaccac ttgtttggcc tggataatgg ccggtacact ctcttcccga atctgtaccc
     2521 cggtggcatg aatgatatct acactttcat tgaacttttt attctttttg aggatgttga
     2581 tgcaggtatg gactactatt ctcctgatcc acccctcaaa tgcacccctg ttttcaaacg
     2641 tatgaatctg taaaaagacc ttaataaatc cttcctgaag catatcctcg gcatcttccc
     2701 ggttctgtgc aaaacgataa cacacggcca gcattttagg actgtacctg ttgtacaatt
     2761 cccgttgggc agtggcatca tttttcaaac atccttttat tatggcttcc tcggtcatag
     2821 ataccctttg gttgcctgta aaatagacaa tttttgggta caaatgctgc acagaagtgt
     2881 aaaaattgag agcaaagggc aaagaaaaag ggtgaaagtc aacgctttca ccctttgaaa
     2941 tggggtttgt ttttaccagt ttgcccgaat ggcaataata aagtttctgc cgggagcgga
     3001 aaagcccgaa gcaaagtacc ggtagtttct gtcaaaaata ttttctatt
//
LOCUS       DAORLS010000348         3038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17669_length_3038_cov_1.840429, whole genome shotgun sequence.
ACCESSION   DAORLS010000348 DAORLS010000000
VERSION     DAORLS010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17669_length_3038_cov_1.840429"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>339)
                     /locus_tag="PK977_17995"
     CDS             complement(<1..>339)
                     /locus_tag="PK977_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20077.1"
                     /translation="NYDDIIMDILYPKSVRTNSSVVYTDPVTLKQYYVRGNPLVSTDC
                     FVRRMDSTLIYRNPLTLVETSKNGSMAYDDGGFSPSEVALFKAWYFADPNIPDVWKYG
                     IGNVGIFKYR"
     assembly_gap    340..393
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<394..1179)
                     /locus_tag="PK977_18000"
     CDS             complement(<394..1179)
                     /locus_tag="PK977_18000"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20078.1"
                     /translation="MKKVSIVQAVSLFAGVFFLSASLLQCTKDGELVKNLDRSYTGGP
                     DSTVYAAFYESNTISTADVVPDVNDVIKMRGVQTIIHEYCNTSNCHGGPIAPRFEIYS
                     QIMQYVKPGDPEGSKLWEYITTNDFDKAMPPVNSSHELGQRDKSIIYNWIKNGAKERP
                     DLNDFRPAAIRLMVDGCSSANCHNTATATGGWARKGLISGLTSADTTQYTYINPTTGS
                     ITVYCQLSNQTLMNQVWTAYKDSVKRFYADTAANASFRPWKTVS"
     gene            1453..1671
                     /locus_tag="PK977_18005"
     CDS             1453..1671
                     /locus_tag="PK977_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20079.1"
                     /translation="MGNDKKNGQKSRRQFFSSFFSDPSKTEKIKMLTPDGKLVEVDKA
                     VFEEAAGKKKASNKDIYNWMKNPSKDNE"
     assembly_gap    1748..1834
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1835..2678
                     /locus_tag="PK977_18010"
     CDS             <1835..2678
                     /locus_tag="PK977_18010"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF20080.1"
                     /translation="GDKVKLLSPDGEIVEIDSSYLNHQEHMAIVSKLEAREGIAGKKF
                     VMVVDLARCKNARKCITACQKWHYRPVETEWLTVKLMKDSEKAAPYWFPKQCFHCDNP
                     PCVKVCPVDATYKRQDGLVLIDNDRCIGCKFCMAACPYSTRVFNWDEPEVPFDIKDLA
                     YNPETGIPAKVGTVEKCDFCPDRAREGLLPPCVESCPNGVFYFGDENEDTVTNGEETI
                     SFTQLIKDRAGYRYMEELGTKPRVYYLPPKDRQFPVERGYEDLEPEIKDRFKDIMEPE
                     KKKS"
     gene            2718..>3038
                     /gene="nrfD"
                     /locus_tag="PK977_18015"
     CDS             2718..>3038
                     /gene="nrfD"
                     /locus_tag="PK977_18015"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRF20081.1"
                     /translation="MELNSYQQQAFDYQRPNIEKFSKRNWFWIIFYLLWIIAGVYALY
                     LQIAKGHGVTGMRDNVVWGLFIVNFIFFIGLSYAGAIIAGLLHLFKVPWGKPIVRIAQ
                     MMTII"
BASE COUNT          874 a          610 c          615 g          798 t
ORIGIN      
        1 tcctgtattt gaagatgcca acattgccaa taccatattt ccagacatcc ggaatattgg
       61 gatcagcaaa ataccatgct ttgaaaagag ccacttctga tgggctgaat ccgccatcat
      121 cataagccat gctaccattc ttagatgttt ctactaatgt cagcgggttg cgatagatca
      181 gtgtagaatc catccggcga acaaaacagt ctgtagaaac cagcgggtta cctcttacat
      241 aatattgttt taatgtaacc gggtctgtat ataccacact ggaattggtt cttactgatt
      301 tgggataaag aatatccata ataatatcat catagttacn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctcacc gttttccatg gacggaagct
      421 tgcattagca gctgtatctg catagaatct ttttacactg tctttataag ctgtccaaac
      481 ctggttcatc agggtttgat ttgacaattg acaatacacg gtaattgatc cggtagtagg
      541 attgatataa gtgtactgag tagtatcagc agaagttaat ccgctaataa gtccttttct
      601 tgcccagcca ccggtagcag tagctgtatt gtggcagttg gcagaactac aaccatcaac
      661 cataaggcga atagcggcag gtctgaagtc attcagatca ggtctttctt tagcgccatt
      721 tttaatccag ttataaataa tgcttttgtc tctttgtccc aactcatggc tggagttaac
      781 tggaggcatt gctttatcaa aatcgttggt agtaatgtat tcccacaact tgcttccttc
      841 cggatcgccg ggttttacat attgcatgat ctgggaatag atttcaaagc ggggtgcaat
      901 cggtccgcca tggcaattgg acgtattgca atactcatga atgatggtct gcacacctct
      961 catcttgatt acatcgttca catcgggaac aacgtctgca gtagaaatgg tgttgctttc
     1021 ataaaaggct gcatacacag tagagtctgg cccgcctgtg tatgatctgt caaggttttt
     1081 tacaagttct ccatccttcg tacactgcaa taaagatgca gacaggaaga atactcctgc
     1141 aaaaagggag accgcttgta cgattgaaac ttttttcata aaaaagctag tttaactgaa
     1201 tgaaattgtt gctataagaa ctggtttcta aaaaagcact gtcaaaagta tttgtgggca
     1261 attgacagtt tcatgacaag caactgacgc aagcatgact ttagtcacac tgactactaa
     1321 agtttatcaa acccccgcat actgtgatat ttctcactta tccccttgac aaacgtcatg
     1381 aaaccgtcag ctattggtaa ataagttgcg gcggtttttt attaaactta aaaaaataaa
     1441 accaacaata aaatgggaaa cgacaaaaaa aatgggcaaa agtcccgtcg gcagttcttc
     1501 tcatctttct tttcagaccc ttccaaaaca gaaaaaatta aaatgcttac ccccgatggc
     1561 aaactggtgg aagtagacaa agctgttttt gaagaagcgg ccgggaaaaa gaaagccagc
     1621 aacaaagaca tctataactg gatgaagaac ccatccaaag acaatgaata accatctgct
     1681 tcactccata tttataatac catgaaacaa gaagaaactc atgacaaagg ccggcgggat
     1741 tttttgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgggga taaagtaaaa ttgctttcac
     1861 ctgatggtga gatagtagaa atagattcct cttacctgaa ccaccaggaa catatggcca
     1921 tcgtctccaa actggaggca cgggaaggca tagccgggaa aaaatttgtg atggtagtag
     1981 acctggcccg ttgtaaaaat gcccggaagt gtattaccgc ttgccagaaa tggcactacc
     2041 gaccggtaga aacagaatgg ctcactgtaa aattgatgaa ggacagtgaa aaagcggcac
     2101 cttactggtt tcccaaacaa tgttttcact gtgataatcc gccttgtgta aaagtttgtc
     2161 ctgttgatgc tacttataaa agacaggatg gcttggtgct gattgataac gaccgttgta
     2221 taggttgtaa attctgtatg gcagcctgtc cttactccac ccgggtgttc aactgggatg
     2281 agccggaagt accatttgac attaaagacc tggcctataa tcctgaaaca ggcattcctg
     2341 ccaaagtagg tactgttgaa aaatgtgatt tctgccctga ccgtgcaaga gaagggctgc
     2401 ttcctccttg tgtggagtct tgtcccaatg gtgttttcta cttcggcgac gaaaatgaag
     2461 atactgttac caacggggaa gaaacgatca gcttcactca actgataaaa gaccgtgctg
     2521 gttaccgtta tatggaagaa ctgggtacca aaccaagagt gtattacctg ccgccaaaag
     2581 acaggcagtt cccggtagag agaggttatg aagacctgga acccgagatc aaagaccgct
     2641 ttaaagacat tatggaacca gaaaagaaaa aatcctgatc tggctgatca caattatcaa
     2701 ccgaacaact cttaattatg gaactcaatt catatcaaca gcaggccttc gactaccagc
     2761 ggcccaatat tgaaaaattc agtaaacgca actggttctg gattatcttc tacctgcttt
     2821 ggatcattgc aggggtctat gctctctacc tgcagatagc caaaggccac ggtgtaacag
     2881 gtatgcggga taatgtggta tggggccttt ttattgtaaa cttcattttc tttattggcc
     2941 ttagttatgc cggtgctatc attgccggtt tgctgcacct gtttaaagta ccctggggca
     3001 aacccattgt tcgtattgca caaatgatga ccatcatt
//
LOCUS       DAORLS010000349         3000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_17954_length_3000_cov_1.842105, whole genome shotgun sequence.
ACCESSION   DAORLS010000349 DAORLS010000000
VERSION     DAORLS010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17954_length_3000_cov_1.842105"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK977_18020"
     CDS             complement(<1..357)
                     /locus_tag="PK977_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20082.1"
                     /translation="MKKLTLFLFALVISVTAFSQHEHHTMQDTMSKRTDTMKNNDFRH
                     TMPSHAYSRNLPMSRNGSGTGWNPDASPMYMWMRSKAKTDWMFHGNIFLRYTSQDVFS
                     SGSRGGDEFSWPNWFMA"
     gene            complement(393..1124)
                     /locus_tag="PK977_18025"
     CDS             complement(393..1124)
                     /locus_tag="PK977_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRF20083.1"
                     /translation="MTHLYQIDGMSCKSCTAKVKSELLKLGEITEAEIQLAYPQAKLT
                     MSNHILVSTLQNAIARAGDYKITETGGMPVHETVGGQSNNWLKTYKPLLLVFAFIIMV
                     ATLASFRDGKFHTMYWMHYFMGGFFIAFSFFKFLDLKGFADSYSSYDLLAAKWYNYGM
                     IYPFFELGLGLAFINNWEPLVTSVVTVALMGFSSIGVIKAVMSKRKIRCACLGAVFNL
                     PMSTVTIVEDLLMVAMGIIMIAGLV"
     gene            complement(1210..>1442)
                     /locus_tag="PK977_18030"
     CDS             complement(1210..>1442)
                     /locus_tag="PK977_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF20084.1"
                     /translation="FSSVTGTTIEQYFILQKIEKVKELLVYDELSLSQMAFKLGYSSV
                     AHLSAQFKKITGLTPSQFKKLNEHHRRHLDSL"
     assembly_gap    1443..1476
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1477..1763)
                     /locus_tag="PK977_18035"
     CDS             complement(<1477..1763)
                     /locus_tag="PK977_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20085.1"
                     /translation="MVCARCIKTVSGIFQSAGAEVKSARLGIIELSGSLSETQQTRVR
                     EQLMAEGFEMLDDQRVKVIEQIKNEIVTLVHYGELDEMNDNLSDFIANKLH"
     gene            complement(1859..>3000)
                     /locus_tag="PK977_18040"
     CDS             complement(1859..>3000)
                     /locus_tag="PK977_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20086.1"
                     /translation="EVYGKAGYVFPEKKYKSIGLQLSAFDHKQDSYFGMTSYNGRQKN
                     FYANLIYQSIIGTTAHKFRTGMSFLSDNYNELFNATRYERQETVPGAFFEYTFTPVEK
                     FNIVAGIRADHNSLFGWFATPRLHVRYEPVKGTTIRLSAGRGQRTANIFAENNSVFVS
                     ARQMSIITSSAGKAYGLDPEVAWNKGISIDQKFRLFNRSANFALDFFRNDFQQQVIVD
                     LEDARQVKFYNLDGKSYSNSFQAEMNLEPVKNLEVRLAYRFFDVKSTYGGQLLQKPFT
                     ARHRAFTNLAYEAGGWKFDYTFNINGSKRITNTSANPPAWQKIPESPSYILMNAQVSK
                     TVGKKHPVDFYIGEENLTNFLQGDVIVSADQPFGPYFDASLIVDL"
BASE COUNT          852 a          680 c          554 g          880 t
ORIGIN      
        1 agccataaac cagttgggcc aactgaattc atctcctccc ctgctgccag atgaaaacac
       61 atcctggctg gtgtaacgca aaaagatatt cccatgaaac atccagtctg ttttggcctt
      121 actgcgcatc cacatataca taggacttgc atcgggattc cagcctgtgc ctgatccatt
      181 gcggctcatt ggcaggttcc tggaataagc atgcgacggc atcgtgtgcc tgaaatcatt
      241 atttttcatg gtgtcagttc tttttgacat tgtatcctgc attgtgtgat gttcatgctg
      301 ggaaaatgcg gtaacagaaa tgaccagggc gaacagaaat aaggtcagct tcttcatatt
      361 tgtaggtttt tgtttcgttg aaaaattcag ttctatacca ggcccgcaat cattataatc
      421 cccatagcta ccatcaaaag atcttcaaca atagtgacag tgctcattgg taaattaaag
      481 acagctccca agcaggcgca gcggatcttt cgtttactca tcacagcctt tatcacacca
      541 atgctgctaa agcccatcaa tgcgactgta accacacttg tcaccaatgg ctcccaatta
      601 ttgataaacg caagtcctaa tccaagctca aaaaatggat aaatcattcc ataattatac
      661 cattttgctg ccagcaagtc gtagctgctg taactatcag caaagccttt caggtctaaa
      721 aacttgaaaa aagaaaacgc aataaaaaat ccacccatga aataatgcat ccagtacatc
      781 gtatgaaatt tgccatccct gaatgaagca agggtggcca ccatgataat aaaagcaaag
      841 acaagtaaca gtggcttgta agtcttcagc cagttattgc tctgaccacc aaccgtttca
      901 tgaaccggca tgccaccagt ttcagtaatt ttataatcac ctgccctggc aatcgcattt
      961 tgcagtgtac tgactaaaat atggttgctc attgtcaact ttgcctgtgg ataggccaac
     1021 tgaatctctg cttcggtgat ttccccaagt tttagcaatt cgctttttac tttagctgta
     1081 cagcttttgc aactcattcc gtcaatctga tataaatgtg tcatattggt aagtttctga
     1141 cacaaatttc agctagacac tgcctgccac tgttatacaa ttctgtataa gtgttatatc
     1201 attttaaggt cacagggaat cgagatggcg gcgatggtgt tcattcagtt ttttaaactg
     1261 gctgggggtg aggccggtta ttttcttgaa ctgggcagaa agatgtgcca cactgctata
     1321 gcccagtttg aaagccatct ggctgagaga aagttcatca taaacaagca gctcttttac
     1381 tttctcaatc ttctgtaaga taaaatactg ctcaatcgtt gttccggtaa cggatgaaaa
     1441 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttat gaagtttatt agcgataaaa
     1501 tcagagagat tatcattcat ctcgtctaat tcaccatagt gaacgagggt aactatttca
     1561 tttttgatct gttcaataac ttttacccgc tggtcatcga gcatttcaaa accttctgcc
     1621 attaattgct ccctgaccct tgtttgctgt gtttctgaca gtgacccgga taattctata
     1681 atacctaatc ttgccgattt aacctcagcc cctgcagatt gaaatatgcc cgataccgtt
     1741 ttaatgcatc gggcacatac catattttta atattataaa ctgacattat taatgatgca
     1801 gcagctattt aaagttactt tattttatac cggaacccgg cataaaacat tctcccggtt
     1861 acaggtccac aatgagcgaa gcatcgaaat aaggaccaaa aggctgatca gcactcacta
     1921 ttacatctcc ctgcaggaaa tttgtcagat tctcttcccc gatatagaaa tcaacggggt
     1981 gcttcttgcc tactgtttta cttacctgtg cattcatcag gatataagaa ggagactccg
     2041 gtattttttg ccaggcaggt ggattagctg atgtatttgt aatacgcttt gaaccattga
     2101 tattaaacgt ataatcaaat ttccagcccc ctgcttcata cgcaaggttt gtgaaagccc
     2161 tatgtcttgc tgtgaaaggt ttttgtaaca actggccacc ataagtggat ttaacatcaa
     2221 agaagcgata agccagtctt acttccaggt tcttaacagg ttccaggttc atttcagcct
     2281 ggaaactgtt agagtaggat ttgccatcca ggttataaaa ctttacctgt ctcgcatctt
     2341 caaggtctac aatcacctgt tgttgaaaat cattcctgaa aaagtcaagt gcaaaattgg
     2401 cactacgatt aaataacctg aatttctgat ctatgcttat tcctttgttc caggctactt
     2461 ccgggtcaag cccgtatgct ttccctgcag acgaagtgat aatactcatc tgtctcgcac
     2521 tgacaaatac actgttattc tccgcaaaaa tattggcagt acgctgacca cggcctgcac
     2581 ttaaccggat cgtagtccct ttcaccggtt cataccgaac atgtaatctc ggtgtggcaa
     2641 accaaccaaa aagactgtta tgatcagccc ttatacctgc cacaatattg aatttctcta
     2701 ccggggtaaa cgtatattca aaaaatgcac cgggaactgt ttcctgtctc tcgtaccggg
     2761 tggcattaaa caactcgttg taattgtcag acaaaaaact catcccggtc ctgaacttgt
     2821 gtgccgtagt accgatgatg gactggtaga tcaggttggc ataaaaattc ttttgccgcc
     2881 cgttgtaaga ggtcatgcca aaataagaat cctgcttatg gtcaaaagca gacaactgca
     2941 acccgatact cttgtatttt ttctccggga atacataacc tgccttacca tacacttcat
//
LOCUS       DAORLS010000350         2962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18241_length_2962_cov_1.763674, whole genome shotgun sequence.
ACCESSION   DAORLS010000350 DAORLS010000000
VERSION     DAORLS010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18241_length_2962_cov_1.763674"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK977_18045"
     CDS             <1..613
                     /locus_tag="PK977_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20087.1"
                     /translation="YANKRIKQLGDAQWHQVYKNDTIDARQFVYVGRTTPQHTGGFNV
                     SSSFKGIRLYAGFDYAFGFVILNNQIVRGLSQVQGSQNSTTDVLKTWSPTNPNGTMPR
                     YYWANQGRNYATDASGNNPAANLWEKGDYVMLRELTLVYDLPAQILSKYLANRIKGLS
                     VNITGTNLAYFSGYSGNFPEFGGVDQGKFPLPKRLTIGVRVTL"
     gene            639..>990
                     /locus_tag="PK977_18050"
     CDS             639..>990
                     /locus_tag="PK977_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20088.1"
                     /translation="MKYNKLLMISWAFILMLGVLSCTKKLDEVVPQDAISKDQALKDP
                     NAARTLYHGTYGLFRSYNSTFFQLGEMRSDIWVDGLFTESVDGGLQNLYRHNISSLNV
                     PFTNWGGFYNLIYNF"
     assembly_gap    991..1151
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1152..2170
                     /locus_tag="PK977_18055"
     CDS             <1152..2170
                     /locus_tag="PK977_18055"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF20089.1"
                     /translation="NTAETYKRRTGADTIMTQIKADIEQSLQLFGPANTLPAGKRVYW
                     NRVASLILKGDVFIWSGTHMGGGNGDITIAKNTLQEVRNLQGATLDLQGNYADIFDPT
                     KKTNNKEIIFAINYELQQAQLGVFGNFLVNSIQASTLSFAQAATPTVASVYPYVNGAN
                     RVGMNQAMINRLTAPPADQRISNTFRVMYSNASPFATRGILLTKYIGTTAGTSQIYNN
                     DFPVYRYADVLLLLAEAKAKLGEDPSAEINAIRQRAYGAGYTPYVNGSITANMNAILE
                     EYLREFIAEGKRWWALRRAGDAYVYANLNPTYFSPTSTAKFLLPLSQAMLTADPLLTQ
                     TQGY"
     gene            2273..>2962
                     /locus_tag="PK977_18060"
     CDS             2273..>2962
                     /locus_tag="PK977_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRF20090.1"
                     /translation="MKIRHLYGLIAAPFTPMHTDGSLNLSVIPAYYRMLKLNGINGAF
                     ICGSTGEGVSMTAREKMAVAEAWAECSRNDEDFKVMTLLGGTSITDCRELAIHAQEIG
                     LYGISFTAPYYFEPVNVSMLAKACMAVAEAAPELPFYYYHIPVLTGVNFPMISLLKEI
                     DGKLPNFAGIKYTHEDFMDFLSCLHFQNGKYDMLWGRDENMLPALSIGAKGAVGSTFN
                     YAAPLYHRLMEA"
BASE COUNT          843 a          549 c          627 g          782 t
ORIGIN      
        1 ttatgccaat aagagaatta aacagctagg cgatgctcaa tggcaccagg tttataaaaa
       61 tgacactatt gatgcaaggc agtttgtgta tgttggccgt actactcctc agcataccgg
      121 tggctttaat gtcagcagtt catttaaagg tattcgtctg tatgcaggat ttgattacgc
      181 atttgggttt gtaattctga ataaccagat tgtaaggggc ctcagccagg tacagggatc
      241 gcagaactct accaccgatg tgttgaaaac atggtcgcca accaacccta atggtacaat
      301 gccaagatat tattgggcta accaaggcag aaattatgca acagatgcca gtggtaataa
      361 tccagctgcc aatctgtggg agaaaggcga ttatgtgatg cttagagaac ttacactggt
      421 gtatgatctg ccggcacaaa ttctaagtaa gtatctggcc aacagaatta aaggcctgag
      481 tgtaaatatt accggtacaa acctggctta tttctcagga tacagcggca attttcctga
      541 gtttggcgga gttgaccagg gcaaattccc gttaccaaaa aggcttacca ttggtgttag
      601 agttacttta taattgttta tcaacaaaaa atactgcaat gaaatacaat aaactattaa
      661 tgatatcctg ggcgttcata ttaatgctgg gcgttctttc ttgtacaaaa aaactagacg
      721 aggtagtacc ccaagatgct attagcaaag accaggcttt aaaagatcct aacgctgcac
      781 ggacattgta ccacggtact tatggtctgt tcaggtctta taacagtaca ttttttcagc
      841 tgggtgaaat gcggtctgac atttgggtag atggtctgtt tacagagtcg gtagatggag
      901 gcctgcaaaa tctctatcgg cataacatca gcagcctgaa tgtgcccttt actaactggg
      961 gtggatttta caacctcatt tataatttta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nataatactg cggaaacata taaacgccgt acaggtgcag atacgatcat
     1201 gacgcagata aaggcggata ttgaacaatc tctacaacta ttcggacctg ccaatacgtt
     1261 gcctgccgga aaaagagttt attggaacag ggtagcttcg ctgattttaa aaggagatgt
     1321 atttatctgg tccggaacgc atatgggagg gggtaatggc gatataacaa ttgccaaaaa
     1381 tacattgcag gaggtcagaa atttacaagg tgcaacttta gatctccagg gtaactatgc
     1441 cgatatattt gatccaacta aaaaaacaaa caataaggaa ataatttttg ctatcaatta
     1501 cgagttgcaa caagcgcagc ttggggtttt tggcaacttc ctggttaatt caatccaggc
     1561 cagcacactt tcttttgcgc aggcggctac accaaccgta gcatcggttt atccttatgt
     1621 aaacggtgca aacagggttg gcatgaacca ggccatgata aacaggctta ctgcaccgcc
     1681 cgcagaccag cgtataagca atacattccg ggtaatgtat tctaacgcat ctccctttgc
     1741 aacaagaggt attttactta caaagtatat tggcacaaca gcaggaacat cccagatcta
     1801 taataatgat ttccctgttt accggtatgc tgacgtactc ttattattag ctgaggcaaa
     1861 agcaaaatta ggcgaggatc cttctgcgga aattaatgca atccggcaaa gagcatatgg
     1921 agcaggttat acaccttatg tgaatggaag tattaccgcg aatatgaatg ctattttgga
     1981 agagtattta agagagttta ttgcggaagg taaacgctgg tgggcattaa ggagggccgg
     2041 ggatgcttat gtatatgcaa atctgaaccc tacctatttt tcaccaacta gtacagctaa
     2101 gtttttgttg ccgctttctc aggcaatgtt aacggcggat ccattactta cccaaacaca
     2161 ggggtattaa aaatagtttt tcatttagca acataagcag tcaaagaccg ggggcataat
     2221 tgcctccggt tattcaacta atttttaatt taccaaggct aaataaccga agatgaaaat
     2281 tcgacattta tatggattga tagcggcacc ctttacaccc atgcatacag atgggtcact
     2341 aaatttatct gtgataccgg cttattaccg tatgctaaaa ttaaatggta tcaacggtgc
     2401 ctttatttgc ggctctactg gtgaaggagt ttctatgaca gcaagagaaa agatggcggt
     2461 tgccgaagcc tgggccgaat gttccaggaa cgacgaagat tttaaagtca tgaccttact
     2521 tggaggcacc agcataacag attgcaggga actggccata catgcccagg aaataggttt
     2581 gtatggaata tcttttactg ctccttacta tttcgaaccg gtgaatgtgt ctatgctagc
     2641 aaaagcttgc atggcagtgg ccgaagctgc tccggagtta cctttttatt attatcacat
     2701 tccggtgctc acaggagtta attttccaat gataagcctg ttgaaagaaa tagacgggaa
     2761 gcttcccaat tttgccggga tcaaatatac ccatgaagat tttatggatt ttctttcctg
     2821 tcttcatttc cagaatggga aatatgatat gctttggggc agggacgaaa atatgcttcc
     2881 ggcattatcc attggggcaa agggggctgt cggtagcaca ttcaattatg ctgcacctct
     2941 atatcacagg ctaatggaag ca
//
LOCUS       DAORLS010000351         2946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18379_length_2946_cov_1.562435, whole genome shotgun sequence.
ACCESSION   DAORLS010000351 DAORLS010000000
VERSION     DAORLS010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18379_length_2946_cov_1.562435"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1513
                     /locus_tag="PK977_18065"
     CDS             <1..>1513
                     /locus_tag="PK977_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF20091.1"
                     /translation="VAGMFLAFKSINAGAKTPVNPPSKYEQIMKLVGEMLSQGHYSPQ
                     DINDAFSQKVFAKYMKELDPEKNFFLKGDLDALKKFETRVDDEIKGAPVEFFLAAGKT
                     FTTRMEQASAIVNEFLTKPFDFTVDEEVMLDADKLQYPATEKEIKERWRKKLKYMTLE
                     RYADLLDTRDKNKGKEGFVVKTDEELEKEAREKVKRIMDRTFERYRFKFSDDDKFNLY
                     VNAITTTMDPHTEFFPPVDKRYFDEEMSGRFYGIGASLQYDDGNIKVASVLTGSPAWK
                     SGEIQAGDVIVKVAQGKEEPVELTGFVVTDAVKLIRGKKGTEVKLTLKKTDGSLKVVS
                     LIRDEIVQDETFARSAIVNNNGSKIGYIFLPEFYADFDRPNGNRSFTDVANEVKKLKE
                     EKVDGIVIDLRNNGGGSLYDVVQMAGLFIDEGPIVQVKDRENKPSVLKDKDRNVLYTG
                     PLAVMVNEFSASASEIFAAAIQDYNRGVIIGSTSTYGKGTVQRNIGLDPENGFS"
     assembly_gap    1514..1545
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1546..>1835
                     /locus_tag="PK977_18070"
     CDS             <1546..>1835
                     /locus_tag="PK977_18070"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRF20092.1"
                     /translation="GTIKLTLQKFYRINGGSTQLKGVTPDIVLPDNLEYLKVREKDDE
                     DALPWDEISKAPYNTWKPGYDLKTIQEFSNQRLENDNTFKLIKESSEWLSKK"
     assembly_gap    1836..1848
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1849..2153
                     /locus_tag="PK977_18075"
     CDS             <1849..2153
                     /locus_tag="PK977_18075"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRF20093.1"
                     /translation="DKQYSLKLDKYRAELKTIRTTQKQLESLLKLNTELNVAALPNEV
                     NRWDDDKNKQERFNQWLKNLQKDIYLDQALKVMSDMISQQNLVKSKETTEPVKKAF"
     gene            2262..2918
                     /locus_tag="PK977_18080"
     CDS             2262..2918
                     /locus_tag="PK977_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20094.1"
                     /translation="MLRVKKIILLVLLAIAFNAGAQTKEIYTHPKFDSIAHNHTQLAI
                     LPFDVLINLRPKEKEKLKPGELEQIQKKEGEAVQSAIQTYFLKKKEKEDFKVNFQDIS
                     KTNALLLKNGWTIDSLRAKTKEEVCTLLGVDGLISGTLNTDKPMSEGASIALGVAFGF
                     WGPTNSGKCTINVHEAKEGELLWKYEKTLSRSLGSDINTIINTMMRKASKKFPYEDIK
                     "
BASE COUNT          975 a          521 c          686 g          719 t
ORIGIN      
        1 tggtagctgg tatgttcctc gcttttaagt ctatcaatgc cggtgcaaaa acccctgtga
       61 acccgccttc taaatacgag cagattatga aactggtggg agagatgtta tcccagggtc
      121 attacagccc gcaggacatc aatgatgcct tttcccaaaa agtatttgct aaatacatga
      181 aggaactgga tccggagaag aacttttttc tgaaaggtga ccttgatgct ttgaagaaat
      241 ttgaaacccg tgtggatgat gagattaaag gggcgcctgt cgaatttttt ctggctgccg
      301 gtaaaacctt tactacccgt atggagcagg cctctgctat tgtcaatgaa tttttgacaa
      361 agccattcga ttttacggtt gatgaggaag ttatgcttga tgctgacaaa ctgcaatacc
      421 cggccacaga gaaagaaata aaagaacgct ggagaaaaaa gctgaaatat atgaccctcg
      481 aacgctatgc cgaccttttg gatacccggg ataaaaacaa gggcaaagag ggctttgtgg
      541 taaagacaga tgaagaactg gaaaaagagg cgagagaaaa agtgaagcgg atcatggaca
      601 gaacttttga acgataccgc ttcaaatttt cagatgacga taaattcaac ctgtatgtga
      661 atgctattac tactacgatg gatccgcata ctgagttttt tccaccggtt gataaacgat
      721 attttgatga agaaatgagc ggaaggttct atggtattgg cgcttcactt caatatgatg
      781 atggtaatat taaagtggcc agtgtactta ccggtagccc tgcctggaag tctggtgaaa
      841 tacaagccgg tgatgtgatt gtaaaagtgg cgcagggcaa agaagaacct gttgaactga
      901 ccggattcgt tgtaactgac gcagtaaaat tgatcagggg taaaaaaggt actgaagtaa
      961 aacttaccct gaaaaaaaca gatggttcct taaaagtagt atcactgatc cgtgatgaaa
     1021 tagtacagga tgaaaccttt gcccgtagcg caatcgtcaa taacaatggt tctaaaatcg
     1081 ggtatatctt tcttcccgag ttttatgctg atttcgatcg tcctaatggc aaccgcagct
     1141 ttacggatgt ggccaatgaa gtaaagaaac tgaaggaaga aaaagtagac ggtattgtga
     1201 ttgatctcag gaataacggt ggcggatcat tgtacgatgt ggtacaaatg gccggtttgt
     1261 tcattgatga aggcccgatt gtacaggtta aagacaggga gaataaacca agtgttttaa
     1321 aagataagga cagaaatgtt ttgtacacag gcccgctggc ggtaatggta aacgaattca
     1381 gcgcttcagc ctccgagatt tttgcggcag ctatacaaga ttataaccgt ggtgtaatta
     1441 ttggcagcac ttcaacttac ggtaagggaa cagtgcagcg gaatataggg ctggatccgg
     1501 aaaacggttt ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggta ccatcaaact
     1561 gacattacag aaattctacc gtatcaatgg gggctctaca caactgaagg gagtaacgcc
     1621 ggatattgtg ctgccagata acctggaata tttgaaagta agggaaaagg acgatgaaga
     1681 tgctttgcca tgggatgaga tcagtaaggc accgtacaat acatggaaac ccggatatga
     1741 cctgaaaacc atccaggaat tcagtaacca gcgtttggaa aatgataata cattcaaatt
     1801 gatcaaagag agcagtgagt ggttgtccaa aaaaannnnn nnnnnnnnat gacaaacaat
     1861 attcattgaa actggataag tacagggcag aactgaaaac gatccgcact acgcaaaagc
     1921 aattagaatc tttgctaaaa ttaaatactg aactgaatgt ggcggccctg cctaatgaag
     1981 tgaaccgctg ggacgatgat aagaacaaac aggaacgctt caaccagtgg ctgaaaaacc
     2041 tgcagaaaga tatttatctc gaccaggccc tgaaagttat gagtgacatg attagccagc
     2101 aaaacctggt caaatcaaaa gaaacaacgg agccggttaa aaaggcattc tgattaagaa
     2161 agtatttcaa atttgttaaa agctccttcg ggggcttttt tcacatatat atgttagttg
     2221 atcttcaaac ggctgcaata gttttgtcaa aacgtttaac catgctacga gtaaaaaaga
     2281 taatattact agttctgctg gcgattgctt tcaacgccgg cgcacaaacg aaagagattt
     2341 acacccatcc aaagtttgat tctattgcac acaatcatac ccagttggcc atattgccct
     2401 ttgatgtgct gatcaacctg cgtccaaaag aaaaagagaa actgaaaccg ggagaactgg
     2461 agcagattca aaagaaagaa ggagaagcgg tgcaaagtgc gatacaaact tatttcctga
     2521 agaaaaaaga gaaggaagac tttaaagtca atttccagga tatcagcaaa accaatgcgc
     2581 tgttgttaaa gaacggctgg acaatagatt ctttacgggc aaaaacaaaa gaagaagtct
     2641 gcactttgct gggtgttgac gggctgattt cgggaacatt gaatacagat aagcccatgt
     2701 ctgagggtgc ttctattgca ttgggtgtgg cttttggttt ttgggggcct accaattccg
     2761 gaaaatgtac tatcaatgta catgaagcaa aagaagggga gttgttatgg aagtacgaaa
     2821 aaacattatc acggagcctt ggaagtgata tcaatacgat aatcaataca atgatgcgaa
     2881 aagcttcaaa gaaatttccc tacgaggaca tcaaataaac attagcgtac ataatagaaa
     2941 aaggtt
//
LOCUS       DAORLS010000352         2945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18389_length_2945_cov_1.795502, whole genome shotgun sequence.
ACCESSION   DAORLS010000352 DAORLS010000000
VERSION     DAORLS010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18389_length_2945_cov_1.795502"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..880
                     /locus_tag="PK977_18085"
     CDS             242..880
                     /locus_tag="PK977_18085"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRF20095.1"
                     /translation="MSGFTAEEIPFEQQPLPYAYNALESVIDTMTMEIHYSKHAAAYA
                     KNLADALKAESVDKSSEKLSSLLGKISKYSAKMRNNGGGHYNHEFFWKSMKSPVESNS
                     PQSAIMKAFIKDFGSFDAFKNQFAEAGKNRFGSGWAWLVKTADKKLVVGSTPNQDNPL
                     MDVSELKGTPLLGLDVWEHAYYLKYQNRRPDYINAWWNVVDWKVVQKRFDAA"
     gene            complement(882..1733)
                     /locus_tag="PK977_18090"
     CDS             complement(882..1733)
                     /locus_tag="PK977_18090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRF20096.1"
                     /translation="MDQKIINLYDEYTHKPLSRQEFLRKLSLLAGGTAAAMALLPLLE
                     VNYAHAAVTSEEDLFTERITYPGINGDMQAYVARPKKDKKYSAVVVIHENRGLNAHIE
                     DVARRAAKAGFLAIAPNALSSLGGTPENADEARSKFQQLKAEDNLQNFIKVFDYLPGR
                     KDCNGKYGCVGFCWGGAMSNNLAVNVPSLKAAVAFYGRQPAATDVPKIKAAIQLHYGE
                     MDEGVNKGIAAYEEALKNNKITYELYMYPGAQHAFHNDTAPTRYNETAARLAWQRTID
                     FFNKYLD"
     gene            1878..>2945
                     /locus_tag="PK977_18095"
     CDS             1878..>2945
                     /locus_tag="PK977_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF20097.1"
                     /translation="MKTTNFPYCVRTVIFIALAATYLTACQREIDGPGGGSPVSPGVN
                     DNEMVTGGVTGIVVDENDRPVSGATVISGTNSTTTDRYGSFRFRNISLSKANGTVKII
                     HNGYFNAYRSFISVAGRINNVRIKLIPKTNAGNFSAASGGTISISGGAKLVIPANAIA
                     DAGGAAYVGIVNVAMAWIDPSSNDLPNVLMGDLRGITTDNQERGLSTYGMIGVELTGT
                     GGQPLKIAAGKTAELTFPIPASLQGNAPATIDLWHFDEATARWKQEGTATKTGSNYIA
                     QVNHFSFWNCDAPFPLVDLCMSFKDGNGLPLINAQVRIKRTVNNTYGYGRTDSVGNLC
                     GKVPKDEALVLEVLDQCYNVVY"
BASE COUNT          858 a          571 c          636 g          880 t
ORIGIN      
        1 aataatctta taacataaat caataaacat atgtttaaat atgctttacc tttaatcatc
       61 tgttttttta taaaaaaaaa tctggtttat gaagaaaagt gtaaagcata tttaaacata
      121 tgtttattga tttatgaaga aaagtgtaaa aaatagaaga gagtttctaa aaacgaccgg
      181 tatgatcagt gtttctgctt ttgtattgcc ttcatgggct tgtgctgcgg gcaaaactgc
      241 catgtcaggt ttcactgcag aagaaattcc atttgagcag cagccacttc cttatgctta
      301 caatgccctg gaatctgtga ttgatacgat gacaatggag atccactatt ctaagcatgc
      361 agctgcttat gccaagaacc tggcagatgc tttgaaagct gaatcagttg ataaaagctc
      421 tgagaagctt agttctttat taggcaagat ttctaaatat tcagctaaaa tgagaaacaa
      481 tggcggtggg cactataacc atgaattttt ctggaaaagc atgaaaagtc ctgttgaatc
      541 caattcaccg caatctgcta tcatgaaggc tttcataaaa gatttcggtt cttttgacgc
      601 ttttaaaaat caatttgccg aagcaggtaa aaatcgtttt ggcagcggtt gggcatggct
      661 ggtaaaaact gctgataaaa agctggtagt aggttctaca cctaaccagg acaacccact
      721 tatggatgta agtgaattaa aaggaactcc attgcttgga ctggatgtat gggaacatgc
      781 atactatttg aaataccaaa accggagacc tgattatatc aatgcctggt ggaatgtggt
      841 ggactggaaa gttgtgcaaa agcgttttga tgcagcctga tctaatccag gtatttatta
      901 aaaaaatcaa ttgtcctctg ccaggctaac ctggcggcag tttcattata ccgggtgggt
      961 gccgtatcat tatgaaaagc atgctgtgcg ccggggtaca tatacaattc gtaggtgatc
     1021 ttattatttt ttaatgcttc ttcataagca gcaattcctt tgtttactcc ttcatccatt
     1081 tcaccataat gcagctgtat ggcagccttt atttttggaa catctgtggc agccggttgt
     1141 cgaccataaa aagctactgc tgctttcagg gaaggaacat ttaccgctaa attattgctc
     1201 atggcacctc cccagcaaaa gcccacacat ccatatttcc cgttgcaatc tttccggcct
     1261 ggcaggtaat caaatacttt aataaaattt tgcaggttgt cttctgcctt tagctgctga
     1321 aatttagacc ttgcttcatc agcattttca ggtgtgccgc ccaatgagga taaggcattt
     1381 ggcgcaatag cgaggaaacc ggctttggca gctcttctgg ctacatcttc tatatgtgca
     1441 ttcaaacccc tgttttcatg tatcactact acggcagaat attttttatc ctttttgggt
     1501 cttgccacat aggcttgcat atctccatta attcccgggt aagtaattct ttcagtaaaa
     1561 agatcttctt cagaagtcac cgcagcatgg gcatagttta cttccaatag gggtaacaaa
     1621 gccatggctg ctgcagtgcc tccggccaac aggctaagtt tacggagaaa ttcctgcctg
     1681 ctcaaaggtt tatgtgtgta ctcgtcgtag agattgatta ttttctgatc cattgttttt
     1741 tatttgtctt taaagttata tagaactatc atccggggaa gaaaaaatat agttcgttga
     1801 tcatcgcata aatatcgctt tgtgatgaaa aaagctgagt atatttatga caagtttact
     1861 taatacaacc agcaatcatg aaaactacca acttcccata ctgtgttagg acagtcattt
     1921 ttattgccct ggctgcaaca tatctcactg catgtcaaag ggaaatagac ggtccgggtg
     1981 gcggctcacc tgttagcccc ggtgtaaatg ataatgaaat ggtgaccgga ggggttactg
     2041 gtatcgtggt tgatgaaaat gacagaccgg tttccggggc tactgttata agtggtacaa
     2101 actctacgac aactgaccgc tatggttctt tccgtttcag gaatataagt ttatcgaaag
     2161 ctaatggaac agtaaagatt atacacaacg gttactttaa cgcctacagg tcttttattt
     2221 ctgttgccgg aaggatcaat aatgttcgga taaaattaat tcctaaaacc aatgcaggta
     2281 atttttctgc tgcctcagga ggaacgatca gtatttcagg aggggcaaaa ctggtaatcc
     2341 ctgctaatgc aattgcagat gcaggtggtg ctgcctacgt tggaatcgta aatgtggcta
     2401 tggcatggat tgacccttct tcaaatgacc ttcccaatgt actgatgggt gacctcagag
     2461 gaattaccac tgataaccag gaaagaggac tttccacata tggaatgatc ggggtggagc
     2521 taaccggcac gggagggcag ccgttgaaaa tagcagcggg gaaaacagca gaactcactt
     2581 ttccgattcc ggcttctttg caggggaatg cgccggctac aatagattta tggcattttg
     2641 atgaagcaac tgcaagatgg aagcaggagg gaacagctac aaagactggt tcaaactaca
     2701 ttgcacaggt aaatcatttc tctttctgga actgtgatgc gccatttcca ctcgttgacc
     2761 tttgtatgtc attcaaagat gggaatggtc tgccgcttat taatgctcag gtccgcatta
     2821 aaagaacagt aaacaatacg tatggttatg gcagaacaga ttctgttggt aacctttgcg
     2881 gaaaagtacc taaagatgaa gcacttgttc tggaagtact tgatcaatgt tataatgttg
     2941 tttac
//
LOCUS       DAORLS010000353         2940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18428_length_2940_cov_1.951820, whole genome shotgun sequence.
ACCESSION   DAORLS010000353 DAORLS010000000
VERSION     DAORLS010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18428_length_2940_cov_1.951820"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PK977_18100"
     CDS             <1..1248
                     /locus_tag="PK977_18100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20098.1"
                     /translation="DITNGGVYSGATTATLNISGATTGMSGYQYRCMLSNATCTTPGT
                     SNAATLTVNSLPAVSTNPQNATICEGGSATFSVTATGTSISYQWQLSTDGGTTYNNIA
                     GANTNSYTVSAVTTGMNGNRYRCVVSGTCSPAVTSTAAILTVIAPVTVTSQPANAERC
                     SGSNATFNVTGSGAGVIYQWQVSTDGGANWTNISGATNSSLTINAVTTGMNNYRYRCL
                     LSNNICTNPVASSGALLTVRQLPTVSLNAASLTSLLPGQTTTLTAVPSASTGGTLTTS
                     WSLNGSTFSNTTNSYVVNIENTGSYQVSIQEVFAGGLTCSNQSAVVTIDATISNKLFI
                     FPSPNDGNFTVSYYNYGGASTQRSISIFDSKGSRVYNGQFTVTGPYTLIPINLQKANT
                     GIYYVVIGDAAGKKLAEGKVHVH"
     gene            1340..2410
                     /gene="serC"
                     /locus_tag="PK977_18105"
     CDS             1340..2410
                     /gene="serC"
                     /locus_tag="PK977_18105"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRF20099.1"
                     /translation="MIHNFNAGPSILPKEVFEEASRAILNFNDTGLSLLEIGHRTPLF
                     QPVMEEARSLVKELMQLDNDHEVLFLHGGATTQFMQVPMNLLNENDVAAYTETGTWAN
                     KAIKEAKLFGHVEIAASSKDSNYTTIPKDLPVSPTAAYLHITTNETIAGTQWHKIPFD
                     CGVPIVADMSSDIMSRVLDFNKFDVIYAGAQKNIGAAGVNLVVINKNILGKVNRSIPT
                     ILDYKNHIKEASMLNTPPVFAVYVAMLTLRWLKKKGGVEAIEKINNEKAALFYNTLDS
                     LPIFKAPVAKEDRSKMNAVFVMDNELLEKEFLDWCKNEGMVGVKGHRSVGGFRVSMYN
                     ALPLESVKALTQLMKEFALKKG"
     gene            2414..>2940
                     /locus_tag="PK977_18110"
     CDS             2414..>2940
                     /locus_tag="PK977_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRF20100.1"
                     /translation="MAIIKPFRALRPKSELSQLVASRPYDVLNSDEARTEAKDNPFSF
                     LHVTKSEIDLAPETDIHSVAVYEKAKENLQELIKHGILFQEDKDCYYIYQLIMNGRSQ
                     TGLVCVSSVEDYFNDVIKKHEFTRPEKEKDRIDHMRTIEAQTGNVFLAYRDVMEINAI
                     INGWKMTNKLVYDFTA"
BASE COUNT          942 a          600 c          621 g          777 t
ORIGIN      
        1 gatatcacta acggcggtgt atattcagga gctactactg caacactaaa tatctcaggc
       61 gctaccaccg gtatgagcgg ataccagtat cgttgcatgt tatcaaatgc tacttgtaca
      121 acaccaggta catccaatgc tgcaacatta actgtcaatt cattgccggc tgtttctact
      181 aatccccaga atgcaactat ctgtgaaggg ggtagtgcta ccttctcagt aacggctact
      241 ggcacaagta tctcctacca gtggcaattg agcactgatg gcggaacaac atataataac
      301 atagcaggag caaataccaa ttcatataca gtttcagcag tcacaaccgg tatgaatgga
      361 aacaggtatc gttgtgttgt gagcggtaca tgttcgccgg ccgtgacttc cactgctgct
      421 atattaactg taattgcgcc agtaaccgtt acaagtcagc ccgccaatgc agaaaggtgt
      481 tccggcagta acgccacatt caatgtaaca ggaagcggcg ctggagtgat ttatcaatgg
      541 caggtaagta cagatggcgg tgccaactgg accaatatca gcggagctac taattcaagc
      601 ctgacaatta atgcagtcac caccggcatg aataattacc gatatcgctg cttgttatcc
      661 aataatattt gtaccaatcc ggttgcctca tccggggcac tgctcactgt acggcagtta
      721 ccaactgttt ccctaaatgc tgcttcttta acaagcttgt tgccagggca aacaaccact
      781 cttacagcgg taccaagtgc ttctacaggg ggaacattga cgacgtcctg gtctttgaac
      841 ggctcaacat ttagtaatac cactaacagt tatgttgtaa atattgagaa taccggctct
      901 taccaggtca gtatacaaga agtgtttgcc ggcggactta cctgttctaa tcagtctgct
      961 gttgtaacta ttgatgctac tataagcaat aagctattca tcttcccttc accaaacgat
     1021 ggaaacttta ccgtttctta ttacaattat ggaggagcca gcacacaaag atcaatttcc
     1081 atctttgaca gcaaaggttc aagagtgtac aacggacagt ttactgtaac agggccttac
     1141 acattaattc caatcaattt gcaaaaagca aataccggaa tttattatgt tgtaattggt
     1201 gatgctgctg gtaaaaaact ggcagaagga aaagtacatg tacattaaat aaattaatgc
     1261 ccttataaaa atcccttata gtttcttctg taagggattt tttatggaca gtaaaacctg
     1321 cattcaatac ctttgcgcca tgattcacaa tttcaatgcg ggcccgtcaa ttttgccgaa
     1381 agaagttttt gaagaagcca gcagggctat actcaatttc aacgatacgg gtctttctct
     1441 cctggaaata ggtcaccgga cgccattatt ccagcctgta atggaagagg ccagaagtct
     1501 tgtaaaagaa ttaatgcagc tggataatga ccatgaagtg cttttcttac atggtggggc
     1561 cacaacacaa tttatgcagg tgcccatgaa cctcttaaat gaaaatgatg ttgcagcata
     1621 taccgaaacc ggaacatggg caaacaaagc cataaaagaa gctaagcttt tcgggcatgt
     1681 agaaatcgct gcatcatcca aagacagcaa ttataccaca atacctaagg atttgccggt
     1741 gagtccaacc gctgcctacc ttcatattac taccaatgaa accattgcag gcacacaatg
     1801 gcataagata ccatttgatt gtggtgtgcc aatagtagca gatatgagca gtgatattat
     1861 gagccgggta cttgatttca acaagttcga tgtcatctat gcaggtgcac aaaaaaatat
     1921 cggggcagcc ggtgtcaacc tggttgtaat aaataaaaat atcctgggca aagtgaaccg
     1981 gagcatcccc actattcttg actacaagaa tcacatcaag gaagcaagta tgctgaatac
     2041 acctcctgta tttgcggtat atgtggccat gcttaccctg agatggctaa aaaagaaagg
     2101 tggtgttgaa gctatagaaa aaattaataa cgagaaagct gcattatttt ataacacatt
     2161 ggacagttta cccattttca aagctcctgt tgcaaaggaa gacagaagta aaatgaatgc
     2221 cgtgtttgtt atggataatg aattactcga aaaagaattt ctcgactggt gtaagaatga
     2281 aggcatggta ggtgtaaaag gacatcgcag cgtaggggga ttcagggtat ctatgtacaa
     2341 cgctttacca ttagaaagtg tcaaagcgct aacacaatta atgaaagagt ttgcattaaa
     2401 aaaaggataa taaatggcga tcattaaacc gttcagggca ttaagaccaa aatcagaatt
     2461 atctcaactt gtagcatcaa gaccctatga tgtattgaac agcgatgaag caagaacaga
     2521 agcaaaggat aatccctttt cttttttaca tgtcaccaaa tcggaaatag accttgctcc
     2581 agaaactgac attcacagtg tggctgtgta tgaaaaggca aaggaaaatc tacaggaatt
     2641 gatcaaacac ggtattcttt tccaggaaga caaagattgt tactatatat accagttgat
     2701 aatgaacggt cgcagtcaga caggacttgt ttgtgtttca tctgttgaag actatttcaa
     2761 cgacgttatc aaaaagcatg agtttacaag acccgagaaa gaaaaggatc gtattgatca
     2821 tatgcggacg attgaagcgc aaaccggcaa tgtgtttttg gcttatcggg atgtgatgga
     2881 gatcaatgcc attatcaatg gctggaaaat gaccaataag ctggtttatg atttcacagc
//
LOCUS       DAORLS010000354         2930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18487_length_2930_cov_1.863304, whole genome shotgun sequence.
ACCESSION   DAORLS010000354 DAORLS010000000
VERSION     DAORLS010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18487_length_2930_cov_1.863304"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>314)
                     /locus_tag="PK977_18115"
     CDS             complement(<1..>314)
                     /locus_tag="PK977_18115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF20101.1"
                     /translation="MYGAIGGEANAIMPGKRMLSSMTPTIVLKDNKPYIVVGTPGGTT
                     IPTQIFQTLVNILEFNMSTEDAVYKPKFHHQWLPDEVIVEKGFPAAVKEAMQKMGYTF
                     GK"
     assembly_gap    315..324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<325..>634)
                     /locus_tag="PK977_18120"
     CDS             complement(<325..>634)
                     /locus_tag="PK977_18120"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF20102.1"
                     /translation="DFYKVPVDMLTNEVYLQERMKSYDPQKATPSSSIGAGKLSGTES
                     EETTHLSVIDKEGNAVAVTTTLNNSYGSRTVVGGAGFFLNDEMDDFSIKPGVPNMYGA
                     V"
     assembly_gap    635..714
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<715..>1098)
                     /locus_tag="PK977_18125"
     CDS             complement(<715..>1098)
                     /locus_tag="PK977_18125"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF20103.1"
                     /translation="LNNLQDDLKKYNSIMPVFVKNEKWNTGDTLVQKDLANTLKRIKQ
                     NGAAGFYEGETAKLVAEEMKRGGGIITEEDLKNYKAKPRIPHSFTYKGYKIVGMPMPS
                     SGGLLLHQMMKMIEDRNISSMGFHSV"
     assembly_gap    1099..1194
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1195..1666)
                     /locus_tag="PK977_18130"
     CDS             complement(<1195..1666)
                     /locus_tag="PK977_18130"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF20104.1"
                     /translation="MLRKILYFIVFVLFLSACKTTQKNQVSLYNINSYQYNGSKKVVA
                     ENGAVVSAHPLASKVGVEILKQGGNAVDAAIATQLALAVVYPNAGNIGGGGFFVAKLS
                     NGEAVAIDYREMAPAKAHRDMYLDADGNARTDKSQLGHLSSGVPGTVAGLFESHK"
     gene            1780..>2930
                     /locus_tag="PK977_18135"
     CDS             1780..>2930
                     /locus_tag="PK977_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRF20105.1"
                     /translation="MVRKALLSILSLFIVASVSAQLKSPDDFLGYKIGTRYTPHFKIV
                     NYYNHVAASMPSMVKLEQYGETNEGRPLLLTYVSSPENIKNLESIRQNNIRLANLAKD
                     RMAPQEDNAPAIVWLSYNVHGNEPSSSEAALLTIYSLTDPSNAKTKEWLKNTVVIIDP
                     CLNPDGRDRYVNWFNSAVGKEYNPNPIAREHREPWPAGRTNHYNFDLNRDWAWQTQAE
                     TRYRIKKYLEWMPHVHVDYHEQGVNEPYYFAPAAEPFHEVITPWQRDFQHTIGKNNAK
                     YFDGNSWLFFTKIRFDLFYPSYGDTYPTYNGAIGMTYEQGGISGGLGIINEDGDTLTL
                     VDRALHHYTTSLSTIEASSLNAGKLVKEFRKYFNNAVSNGVGEYKAYIIR"
BASE COUNT          750 a          616 c          535 g          843 t
ORIGIN      
        1 cttttccgaa ggtatatccc attttctgca tagcttcttt aacagccgca ggaaacccct
       61 tctcaactat cacttcatcg ggcagccact ggtgatggaa cttgggtttg tacacagcat
      121 cttcagtact catgttgaat tcaaggatgt tgaccagtgt ttgaaatatt tgtgtgggaa
      181 tggtagttcc gcctggagta cctactacta tataaggttt gttatccttc aaaacaattg
      241 tcggggtcat tgaacttaac attcttttgc caggcattat ggcattggct tcaccaccaa
      301 tggctccgta catgnnnnnn nnnnaacagc gccatacata ttgggcacac caggtttgat
      361 actgaaatca tccatttcat cattcaggaa aaagccagcg cctcccacaa cggtgcggct
      421 gccataagaa ttatttaaag tagttgttac cgctacagca ttgccttctt tatcaattac
      481 actcaggtga gtagtttcct cgctttcagt accggataat ttacctgctc caatacttga
      541 actgggggta gctttttgcg ggtcatagct tttcattctt tcctgcaggt acacttcatt
      601 ggtcagcata tccaccggca ctttatagaa atctnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncactga
      721 atgaaagccc attgaactga tattccggtc ttcaatcatt ttcatcatct ggtgtaacag
      781 taagccgcca ctgctgggca tgggcatacc cactatttta tagcctttgt aggtaaaact
      841 gtgcggtatt cttggtttgg ccttataatt tttcaaatct tcttcagtga ttattccgcc
      901 gcctctcttc atttcttctg ctaccagttt ggctgtttct ccctcataaa atcctgcggc
      961 accattttgt tttattcttt ttaatgtatt ggcaaggtct ttttgcacta atgtgtcacc
     1021 cgtattccac ttttcgtttt ttacaaatac aggcataatg ctgttgtatt ttttcagatc
     1081 atcctgcaga ttattcagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttgt
     1201 gtgattcaaa taaaccggca accgttcccg gtactccgct tgataaatgc cccaactggc
     1261 ttttatctgt tcttgcatta ccatcagcat ccaggtacat atcccgatgt gcttttgctg
     1321 gtgccatttc ccggtagtca atggctactg cttcaccatt actcaattta gcaacaaaaa
     1381 aaccacctcc accaatattg cctgcattcg gatataccac tgccagtgcc agttgtgtag
     1441 caatagccgc atctactgca ttacctcctt gctttaaaat ttctactcca actttactgg
     1501 ccaatggatg tgctgataca acagcgccat tctctgcaac tactttttta gaaccgttgt
     1561 attggtatga gttgatattg tataagctga cctggttttt ttgtgttgtc ttgcaggccg
     1621 acagaaatag aacgaaaaca ataaaatata aaatcttgcg cagcatcaat tagtcttttt
     1681 aagacgacta aactacataa ataagggcat ttgaccggga gtaaaaaagg gtggcaatta
     1741 atcaacgctt tcttaacttg ccggactaaa attttttcta tggttcgaaa ggctttgcta
     1801 tcaattttgt cattattcat tgttgccagt gtttcggcac aactaaaatc accggatgat
     1861 tttttggggt ataagatagg tacccgttat actccgcatt tcaaaattgt taattattac
     1921 aatcatgttg cagctagtat gccctcgatg gtaaagttgg agcaatatgg tgagaccaat
     1981 gaaggaagac ccttattact tacctatgtc tcatcgccgg agaatatcaa aaacctggag
     2041 tcaatccggc agaataatat ccgcctggcc aatttagcta aagaccggat ggcaccgcaa
     2101 gaagacaatg cgccagccat tgtttggtta agttataatg tgcatggcaa tgagccttct
     2161 tcctctgaag ctgccttgct taccatttac tcattgactg atccatccaa tgcaaagaca
     2221 aaagaatggc tgaagaatac tgtcgtaatc attgacccat gtttaaatcc tgatggccgt
     2281 gaccgttatg tgaactggtt caactcagcg gtcggaaaag aatacaatcc taacccgatt
     2341 gccagagaac accgggaacc ctggcctgcc ggcagaacta accattacaa ttttgatctg
     2401 aaccgcgact gggcatggca aacacaagca gaaacccgtt atcgtataaa aaaatacctc
     2461 gaatggatgc cgcatgtaca tgtggactac catgaacaag gagttaatga accctattat
     2521 tttgctcccg cagcagaacc ctttcatgaa gtgatcactc cctggcaaag agattttcag
     2581 catacaattg ggaaaaacaa tgcaaaatat ttcgacggca atagctggtt gttttttacc
     2641 aaaatacggt ttgatctttt ttatccttca tacggggata catatcccac ttacaatggc
     2701 gctattggta tgacttatga gcagggtggt atttccggtg gtcttggcat aattaacgaa
     2761 gatggtgata cactgaccct ggtggacagg gctctgcatc actatactac ttcgctcagc
     2821 acaatagaag cttcttcact caacgcaggg aaattggtaa aggagttcag aaagtatttt
     2881 aacaatgcgg tttcgaacgg agtaggagaa tacaaagctt atattatccg
//
LOCUS       DAORLS010000355         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18560_length_2918_cov_1.703807, whole genome shotgun sequence.
ACCESSION   DAORLS010000355 DAORLS010000000
VERSION     DAORLS010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18560_length_2918_cov_1.703807"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1540)
                     /locus_tag="PK977_18140"
     CDS             complement(89..1540)
                     /locus_tag="PK977_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRF20106.1"
                     /translation="MKIAVAKGDGIGPEIMDAVLKIFNANNVPLDYDFVDMGKWVFDK
                     GFNNGMTPEAKQTIETLGLLFKGPMETPKGKGVKSINVTARKTWNTFANKRDFRTLHG
                     VDTVFSKAGIPIDITVVRENIEDTYGGIEHMLTHDVALSRRFITRPGSMQVIRYAFEM
                     AKQKKARRITCGHKANIMKITDGLFLECFYEVAKECPELKADDIIVDDLCMKLVTRPD
                     TFDVVVLTNLQGDIVSDLCAGLVGGLGFAPSANIGDHISIFEAVHGTAPDIAGKNIAN
                     PTALLLSGLAMLRHVGLTENAAIIENALLYTLETGVHTGDFGDKSTAAVNTTQFADAI
                     ISNFGKKPGIGAREIIPNKPGTPTPLKLEINPMMISKESDKEIIVGVDLFIESDEQPA
                     AIAAKCQRHAGVKFNLINISNRGTQVWPTGSVYTNLVNQYNVRFESIDDEPLNQQDAI
                     GLYVSMSGNFKICSFELLNKWGDKKAYSLAQGQ"
     assembly_gap    1553..1692
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1693..2046
                     /gene="apaG"
                     /locus_tag="PK977_18145"
     CDS             <1693..2046
                     /gene="apaG"
                     /locus_tag="PK977_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRF20107.1"
                     /translation="SVETFYQSDYSNPLQSEFMFAYRITLENHNSFPVKLHRRHWYIF
                     DSNGTHREVEGEGVVGVQPTLQPGENYQYVSGCNLRTEMGKMYGTYEMENLSNKKMFS
                     VKIPAFEMIVPMKNN"
     gene            complement(2043..>2918)
                     /locus_tag="PK977_18150"
     CDS             complement(2043..>2918)
                     /locus_tag="PK977_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF20108.1"
                     /translation="LLTGWWWWAVAIPYFYVSQLYFKGRFGLMFHCLCHRKTLKAPFQ
                     QPFHTYITWILCPLFGHAPEGYFSHHMGMHHIENNMPDDTSSTMYYQRDSLKDFLSYF
                     IKFMFRGVIDTIRYLFVRKRKKLYQRLTIGEYVYILFCIGMCFVNLKATLVVFIVPLI
                     FARLVMMLGNWTQHSFVDAKDPGNLYTNSINCINTIYNKTCWNDGYHIIHHLRPGMHY
                     TEMPGEFLKRKDEFAANKAIVFDGIHYLHVFTWLLTKRYDKLADNLVNINGNMFESEK
                     EAIQLMKERTRKITA"
BASE COUNT          772 a          594 c          584 g          828 t
ORIGIN      
        1 ggaattttgt cctgtaattt aagccagtaa aaagaaaaaa ggaaacgctg ctaacgtctc
       61 ctttttctaa ttatttatgt aattgtattt attggccctg ggcgaggcta taggcctttt
      121 tatctcccca tttattcagc agttcgaaag aacatatttt gaagttgccg ctcatgctaa
      181 cataaagtcc aattgcatcc tgctgattga gcggttcatc gtcaatactt tcaaaacgga
      241 cattgtattg atttaccagg ttggtatata cagagcccgt cggccaaact tgggtacccc
      301 gattgctgat attgatcaag ttgaatttca cacccgcatg acgctgacat ttagctgcta
      361 ttgctgccgg ctgttcatca ctttcaataa acaggtcaac gccaacaatg atttctttgt
      421 ctgattcttt tgaaatcatc atggggttga tttccagttt gagaggtgtg ggtgtgccgg
      481 gtttgttggg aatgatttcc ctggcaccta ttcccggttt cttaccaaaa ttgctgatga
      541 tagcatccgc aaactgtgtg gtattaactg cagcggttga tttatcacca aaatcgcctg
      601 tatgaacccc ggtttctaac gtataaagca atgcattttc aatgattgct gcattttcag
      661 taagtcccac atggcggagc atagccaggc cgcttaacaa caaagcagtt gggttagcaa
      721 tattttttcc tgcaatatca ggcgctgttc catgaaccgc ttcaaagatg gagatgtggt
      781 ctccgatatt agcagaaggt gcaaatccaa gtccgcccac caggccggca cagaggtcag
      841 aaacaatatc gccctgcagg ttggtcagca ccactacatc aaacgtatct ggtctcgtta
      901 ccagtttcat gcatagatca tctacgataa tgtcgtctgc tttcagttcc gggcattctt
      961 ttgccacttc ataaaaacac tccaggaata atccatctgt aattttcatg atattggctt
     1021 tgtggccaca ggtgatgcgg cgggcctttt tctgtttagc catttcaaaa gcatagcgta
     1081 ttacctgcat acttcccgga cgggtgatga aacggcggct taatgccaca tcatgggtca
     1141 gcatatgctc gataccgcca taggtatctt caatattttc tcttactact gttatatcaa
     1201 tggggatacc tgctttactg aaaacggtat ccacaccatg aagtgtccgg aagtctcttt
     1261 tattggcaaa agtgttccag gttttacggg cagtaacatt aatgcttttt acccctttgc
     1321 ctttaggggt ttccatgggc cccttaaaaa ggaggccaag ggtttcaata gtttgctttg
     1381 cttcaggggt catgccatta ttgaagcctt tgtcaaagac ccatttaccc atatccacaa
     1441 aatcgtaatc aagaggtaca ttattggcat tgaatatttt aagtacggca tccattatct
     1501 caggaccgat tccatctcct ttagctactg cgatcttcat aatctatgat ttnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnagtgtgga aacattttac caatctgact acagcaatcc gctccagtca
     1741 gagtttatgt ttgcctaccg cattacactg gagaatcaca actcattccc ggtaaaactg
     1801 caccgccgcc actggtatat ttttgacagc aacggcactc accgggaagt ggagggtgag
     1861 ggtgtagttg gtgtacagcc aactttacag cccggtgaaa attaccaata tgtaagcgga
     1921 tgtaacctgc gaacagaaat gggaaaaatg tacggcactt acgagatgga aaatctcagc
     1981 aataagaaaa tgtttagtgt aaagattcct gcctttgaga tgatcgttcc tatgaagaat
     2041 aactaagcag taatttttct tgtcctctcc ttcatgagtt gaattgcttc tttttccgat
     2101 tcaaacatat tgccgttgat atttacaaga ttatctgcca gcttatcata acgtttggtc
     2161 agcagccagg taaatacatg caggtaatga ataccatcaa aaacaatggc tttattggca
     2221 gcaaattcat ctttcctttt caggaattcg ccgggcattt ccgtgtagtg catacctggg
     2281 cgaaggtggt gaataatatg atagccgtca ttccagcatg tcttgttata tatagtgttg
     2341 atgcagttga tggaattggt ataaagatta ccagggtctt ttgcatcaac aaatgaatgt
     2401 tgtgtccagt tgcccagcat cattaccaga cgggcaaaaa tcaatggcac aatgaatacg
     2461 accagtgtgg ctttcaggtt tacgaaacac atgccaatac aaaaaagtat ataaacgtat
     2521 tcgccgattg tgagccgttg gtataatttt tttcttttgc gaacaaacag atagcggata
     2581 gtatcaatga ctccccggaa catgaatttg atgaaatagg acaaaaaatc tttcaagctg
     2641 tcccgctggt aatacattgt actgcttgta tcatcgggca tattattttc aatgtgatgc
     2701 atccccatgt gatggctaaa atagccttcc ggtgcatgcc cgaacagcgg gcataggatc
     2761 catgttatat aggtgtggaa tggttgctga aagggtgctt tcagtgtttt tctatggcag
     2821 agacaatgaa acattagccc aaatctgccc ttaaaataaa gctgtgaaac ataaaaataa
     2881 ggaatggcaa cagcccacca ccaccagcct gtcagaag
//
LOCUS       DAORLS010000356         2898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18738_length_2898_cov_2.232149, whole genome shotgun sequence.
ACCESSION   DAORLS010000356 DAORLS010000000
VERSION     DAORLS010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18738_length_2898_cov_2.232149"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="nhaD"
                     /locus_tag="PK977_18155"
     CDS             complement(<1..724)
                     /gene="nhaD"
                     /locus_tag="PK977_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HRF20109.1"
                     /translation="MNAVIILIFVLGYLGIAFEHKIQINKAATAMVTGVLCWTIYILF
                     SDNKEVVNDQLSHHLGNLSQILFFLLGAMTIVELIDAHDGFEVITRRITTTDKRKILW
                     VVAIITFFLSALLDNLTTTIVMVSLVRKLVADKNDRWMLAGMIVIAANAGGAWSPIGD
                     VTTTMLWIGNQITAVNVIVKLIIPSLVCLFVPLIAMRRSFKGKIKAPEHIYEYGGTTR
                     STKKERTIVFFTGLAFLLFVPVF"
     gene            845..1339
                     /locus_tag="PK977_18160"
     CDS             845..1339
                     /locus_tag="PK977_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20110.1"
                     /translation="MKKLFYAILLTGSILTAGSVNAQNSGYKTALGVRLSSSNAMINN
                     SVSVKHFLNDKMAIEGLLSFGDPLAIGALVELHQPMTASGLTWYYGGGAYIGFLKKVN
                     TTTQKTTTDPNFGAQGVIGLDYKFNNIPLNLSLDWKPELNIVTDINFEPAAIGFTARF
                     TFGK"
     gene            1499..2248
                     /locus_tag="PK977_18165"
     CDS             1499..2248
                     /locus_tag="PK977_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRF20111.1"
                     /translation="MLKKERQAYILHQVNLHNKVLSSSLCSEINVSEDTIRRDLQELA
                     DEGKIIKVHGGALSHSFNQVHFSTNGVYSQNQKRTIAQKAIGLIGNGMFVLTSGGTTI
                     LEMARSLPPQLKATFISGSIPAILEYMHHPNIEVIMIGDRISKNSKITVGSEAIAKIK
                     QVKADICFLGTNAIDTEQGITDNDWEVVQLKKAMIESSKKVVCLTISEKINSVQPIKV
                     CGLNEIDMLITELSPDDPLLKPYINAGVKVA"
     gene            2292..>2898
                     /locus_tag="PK977_18170"
     CDS             2292..>2898
                     /locus_tag="PK977_18170"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF20112.1"
                     /translation="MRKILQLFFVLSLTLLLLPSAVMAQERTITGTIISDDNKTPLAG
                     VTVRVKGTRKITQTDANGKFSIKVGPGETLQFTYVGYQTEEIKPGSGESLGISLKTAD
                     NTMGEVVVTAMDIKRNPRELGYSVQKVSGSDVQETQRENFLNSLQGRVAGLTLDQTSG
                     VAGASSSVVLRGFNSLSLSNQPLYVVDGVILDNSTINETSSG"
BASE COUNT          903 a          609 c          592 g          794 t
ORIGIN      
        1 tgaataccgg aacaaaaagt aaaaaggcaa gcccggtaaa gaaaacaata gttcgctctt
       61 tttttgtact tcgggttgtt ccgccatact cataaatatg ttcgggggct tttatttttc
      121 ctttaaaaga ccggcgcatg gctataagcg ggacaaaaag acaaaccagg ctgggtatta
      181 tcagttttac aataacattg acagcagtaa tctggtttcc gatccacaac atagtagtgg
      241 taacatcacc tataggcgac catgcaccac cggcattggc agctattacg atcatcccgg
      301 ccagcatcca tcggtcattt ttatccgcta ccaatttcct gaccaatgaa accatcacaa
      361 tagttgttgt aaggttatcc agaagtgctg aaagaaaaaa agtaatgata gccaccaccc
      421 agagtatttt tctcttatct gttgtagtaa tccgccgggt aatcacttcg aagccatcat
      481 gggcatcaat cagctccaca atggtcatag cacctaacag aaaaaataaa atctgggaga
      541 ggttgcccag atgatggctc aactgatcat ttactacctc cttattatct gagaacagga
      601 tatatatcgt ccagcacaat accccggtta ccattgcagt agcggcctta ttgatctgaa
      661 tcttatgttc aaatgcaata cccaggtaac cgagaacaaa aatcaaaatg atcactgcgt
      721 tcataaggaa gcgaaaatac acaagaatgt atatgcatgc ctgatgacga aaggatcaat
      781 tttgcatttt atttagaatt tattggcacc atttttcgtt ttctatgagc taaaacaaat
      841 cgccatgaaa aaactcttct atgctatact gcttaccggc agtatactta ctgctggttc
      901 agtcaatgca caaaattccg ggtataaaac cgcacttggt gtacgactca gcagttccaa
      961 tgcaatgatc aacaattccg tttcagtaaa gcatttcctc aatgataaaa tggccataga
     1021 agggttactt tcatttggcg acccgctggc aattggcgcc cttgtggagt tgcaccaacc
     1081 catgacagca tctggcctta cctggtatta tggcggtggt gcctacatcg gttttttgaa
     1141 aaaagtgaat accactaccc aaaaaacaac aaccgatccc aattttggag cacagggtgt
     1201 aatcggactt gattataaat tcaacaatat ccctcttaac ctttcacttg actggaaacc
     1261 agagctgaat atagtaaccg atattaattt tgagccggct gctattggat tcacagcccg
     1321 gtttacattt ggaaagtaag ttttgaagaa gtctttttaa aagctcatag cttaagcgct
     1381 atgagctttt tttattactg ctttaaattt cccccagatt tccacatctg caaaattttt
     1441 ctggtttttg cgtttttgtg caggaatttg atttatattt agccatcaaa actaacggat
     1501 gcttaagaag gaaaggcaag cctatattct tcaccaggta aaccttcata ataaggtttt
     1561 atcctcttca ctttgttccg aaatcaatgt gtcggaagat acaatccgcc gtgatctgca
     1621 ggaactggct gatgaaggaa aaatcatcaa agtacacgga ggtgccctct cccattcttt
     1681 taaccaggta catttctcca ccaacggggt ttattctcaa aatcaaaaaa gaaccatagc
     1741 tcaaaaggcc attggcctca ttggcaatgg catgtttgta ttgaccagcg gaggtactac
     1801 tattctagaa atggcaaggt cgctaccgcc ccagctaaag gcaaccttta tttcgggcag
     1861 tattcctgct atactggaat acatgcatca tcctaatatt gaggtaataa tgatcggaga
     1921 cagaatttca aaaaactcta aaataactgt tggttctgag gctattgcca aaataaaaca
     1981 ggttaaggca gacatatgtt ttctcggtac caatgccata gatacagagc aaggaattac
     2041 cgataatgat tgggaagtgg tgcagctaaa gaaggccatg attgagtctt ctaaaaaggt
     2101 ggtttgcctg actatttctg aaaagatcaa ctcggtgcag ccaatcaagg tttgcggact
     2161 gaatgagata gatatgctga taactgaact gtcgccggat gatccattac taaaacccta
     2221 tattaatgcc ggggtaaaag tggcataata aaaatggaac tgcttattta ttatatctaa
     2281 aaaaaacagc tatgagaaaa attctccaat tgttctttgt gttatcgctg acgctgctct
     2341 tactgccctc cgctgtgatg gcgcaggaaa gaacaatcac cggtacgatc atttcagatg
     2401 acaataagac accgcttgca ggtgttacgg ttagggtaaa aggcacccgt aaaatcaccc
     2461 aaacggacgc taatggaaaa ttctccatta aagtgggtcc cggtgaaaca cttcagttta
     2521 cctatgtggg ttatcagact gaagaaatta aacccggaag cggcgaaagc ttaggtatca
     2581 gcttaaagac tgctgataat accatgggcg aagtcgtggt aaccgcgatg gatatcaaac
     2641 gtaacccaag ggaattgggt tattccgttc aaaaagttag tggaagtgac gtgcaggaaa
     2701 cgcaaagaga aaacttcctg aacagtttgc agggccgggt agctgggtta acacttgatc
     2761 agacatcagg tgtggccgga gcatcctcat cagttgtatt aagaggcttc aactctctat
     2821 cattaagtaa ccagccactc tacgtggtgg atggtgtaat tctcgataac tccactatta
     2881 atgaaacatc aagtggcg
//
LOCUS       DAORLS010000357         2894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18773_length_2894_cov_1.624163, whole genome shotgun sequence.
ACCESSION   DAORLS010000357 DAORLS010000000
VERSION     DAORLS010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18773_length_2894_cov_1.624163"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..>873
                     /locus_tag="PK977_18175"
     CDS             274..>873
                     /locus_tag="PK977_18175"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF20113.1"
                     /translation="MKKIVLVSVLALSTMFVNAQVTFGVKGGVNFATLGGDDADELEG
                     KKSNTGFYLGGTVNVPISSNFAFQPELIYSANQGMEFRESPLELNYNLGYLNIPLMLQ
                     YRNSGFIAEAGPQIGFLMSAKAKISDGTNTDEEDIKDEFKGTDFGVNLGLGYQFSNGF
                     GLNARYTFGMSNIIDETDVDVKNRVFSVGVFFNFGGKKSK"
     assembly_gap    874..892
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1027..1551
                     /gene="pyrR"
                     /locus_tag="PK977_18180"
     CDS             1027..1551
                     /gene="pyrR"
                     /locus_tag="PK977_18180"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRF20114.1"
                     /translation="MQVLLSSQQLSDTIHKLAGQLAGHHKSTEDVVFVGIQQGGVMVA
                     DEMMKHLEQLYPGKMLQYGQLDITFYRDDIRKEILAPDVMNLPFSIENKTVILIDDVL
                     FTGRTIKAALDALLDYGRPAKVELCVLVDRKEHRQFPIQADYTGAILHTSKADKVKLV
                     KNETGNKEVVLIVA"
     gene            1602..2537
                     /locus_tag="PK977_18185"
     CDS             1602..2537
                     /locus_tag="PK977_18185"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRF20115.1"
                     /translation="MQLSTRHLLGIKDLNKEDIQLILSTAEQFKEVLQRPVKKVPSLR
                     DVTIVNLFYENSTRTRFSFELAEKRLSADTINFAASTSSAAKGETLLDTVQNILSMKV
                     DMVVMRHSASGAPHFLAQHIPAAIINAGDGINEHPTQALLDAFSIREKTAKIEGKNIA
                     IIGDIMHSRVAQSNIYLLKKMGAKVTVCGPPTLIPKYIGEALGVNVSYNLRETLEWCD
                     VANVLRIQLERQNQVLFSSLREYSLTYGINRQLLDSLNKEIVIMHPGPINRGVELDSD
                     VADSKQSIILQQVENGVAVRMAVLYLLSGRKSTGN"
     assembly_gap    2542..2600
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2601..>2894
                     /gene="coaD"
                     /locus_tag="PK977_18190"
     CDS             <2601..>2894
                     /gene="coaD"
                     /locus_tag="PK977_18190"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRF20116.1"
                     /translation="DPLTLGHLDIINRALPLFDKIVVGIGLNSSKAPMFTAEQRLEWI
                     RDIYKDEERVEGAVYEGLTIDYCKKIGAHFILRGIRYVSDFEYEKTIADANRTL"
BASE COUNT          851 a          500 c          627 g          838 t
ORIGIN      
        1 aaaccaactg gtgcagtggt aggacagcaa ccttttggcg gtgcaagagg aagcggcacc
       61 aatgataaag caggtagtat cctgaatctt tatcgctggt tgagtgcaag aacgataaaa
      121 gaaacgtata atccgccagt agattaccgt tatccctttt tgtctgaagt gtaaatattt
      181 ttataaaata tcctgaaaat catttttttg gcgattttac acacatatct ttgcgccact
      241 aaaaaaacct tataaacttt taaatcaatg gttatgaaaa aaatcgtttt agtgtccgtg
      301 ttagcccttt ctacaatgtt tgtaaacgcc caggttactt ttggcgtaaa aggcggtgtt
      361 aactttgcta ctttaggtgg tgatgatgct gatgaactgg aaggtaaaaa gtctaatacc
      421 ggcttttatc tcggtggtac tgtaaatgtt ccaatcagca gcaattttgc ttttcaacct
      481 gaattaattt attctgcaaa ccagggtatg gaattcagag aatcacctct ggaattgaat
      541 tacaaccttg gttatttgaa cattccattg atgttgcaat accgtaacag tggttttatt
      601 gcagaagcag gacctcaaat tggtttcctg atgtcagcta aagcaaaaat atctgatggt
      661 actaacacag atgaagaaga tattaaagat gagtttaaag gaacagattt tggtgtaaat
      721 ctgggtctgg gttaccagtt tagcaacggc tttggtttaa atgcccgtta tacatttggt
      781 atgtctaaca ttattgacga aactgacgtt gacgttaaaa acagggtatt ctctgtaggc
      841 gttttcttca attttggtgg taaaaaatct aaannnnnnn nnnnnnnnnn nngaaatagt
      901 ttatcaaaaa ggccggtttt ccggcctttt tgttttattg ttgaaagtga ttgataaaag
      961 gtattttggt tttttgtgct cctcaattct gctgatgatt tgataacttc accgcaaatt
     1021 tcaaccatgc aggttttgct ttcttcccag caattatcag ataccattca taaactggct
     1081 ggccaattag ccgggcatca taaatcaaca gaggatgttg tgtttgtagg tatacaacaa
     1141 ggaggagtga tggtggcaga tgagatgatg aagcatttgg aacagcttta tcccggcaaa
     1201 atgctgcaat acgggcaact ggatattact ttttacaggg atgatattag aaaagaaata
     1261 ctggctcctg atgtcatgaa tcttcctttt tccattgaaa acaaaacggt gatactgata
     1321 gatgatgtgt tgttcacagg ccgtaccatc aaagctgctt tggacgcatt attggattat
     1381 ggccgtccgg ccaaagttga gttgtgtgta ctggtagaca gaaaggaaca tcgccagttc
     1441 cccatacagg ctgattacac cggagctatt ctccatacca gtaaagcaga taaagtaaaa
     1501 ctggtaaaga atgagaccgg caataaggaa gtggtgctga ttgtggcata ggttctggat
     1561 acttttttga atccaactat ttaccctaat ttcgcccttt aatgcaacta tccactcgtc
     1621 atctgctggg cattaaagac ctcaacaaag aagatatcca gcttattctt tctaccgcag
     1681 aacaatttaa agaagtatta caaagacctg tcaaaaaagt tccttccctt cgtgatgtga
     1741 cgattgtgaa tctcttttat gagaattcta cccggacacg attctctttt gagctggctg
     1801 aaaagagatt gagtgctgac accatcaatt ttgctgcttc cacttcttct gcagcaaaag
     1861 gtgaaacatt gctggataca gtacagaata tcttaagcat gaaagtggat atggtggtta
     1921 tgcggcacag tgcaagcggg gcacctcatt tccttgcgca gcatattcct gctgctatca
     1981 tcaatgcagg tgatggtata aacgaacatc caacacaggc cttactggat gctttttcaa
     2041 taagggaaaa aacagccaag atcgagggga agaacattgc aattattggt gatatcatgc
     2101 acagccgtgt tgcacagagt aatatctatt tattgaaaaa aatgggtgct aaagtgacag
     2161 tatgcggtcc acccacctta attccaaaat acattggcga agctctggga gttaatgtga
     2221 gctataatct gagagaaaca ttggaatggt gtgatgtggc gaatgtgctg cgcattcagt
     2281 tagagcggca gaaccaggta ttattttcat cgcttcggga atacagtctt acttatggta
     2341 tcaaccgcca gttgctcgac agccttaata aagaaatcgt tattatgcac ccggggccaa
     2401 ttaatcgtgg tgtggaactg gatagtgatg tagccgactc gaaacaatcg attatattgc
     2461 aacaggtaga gaatggtgtg gcagtacgaa tggctgttct gtatttactt tcaggaagaa
     2521 aatcaacagg taattaataa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn gatccgctaa cactgggtca tctagatatt attaacaggg
     2641 ctttgccttt gtttgataaa atagtggtgg gtattggtct taattcttcc aaagcaccca
     2701 tgtttacagc agaacaaagg ctggagtgga tcagggatat ttataaagat gaggaaaggg
     2761 tagaaggagc cgtatacgaa ggtctgacta tagactattg caaaaagatc ggggctcatt
     2821 ttattttaag gggcatccga tatgtgagtg actttgagta tgagaaaacg attgctgatg
     2881 ccaaccgaac actt
//
LOCUS       DAORLS010000358         2883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18855_length_2883_cov_2.342645, whole genome shotgun sequence.
ACCESSION   DAORLS010000358 DAORLS010000000
VERSION     DAORLS010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18855_length_2883_cov_2.342645"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1132)
                     /locus_tag="PK977_18195"
     CDS             complement(122..1132)
                     /locus_tag="PK977_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRF20117.1"
                     /translation="MTDTIINLETVNPIEFFGVNNGKLDILKKKFPLLKILSRGTQLK
                     LSGSPEQIESAREKISLIISYLERNGHMSQNYFEQVLGGDDAEVIDHFQEKNPNEVLV
                     FGPNGKSVRARTANQKRLVQVAEKNDIVFAIGPAGTGKTYTAVALAVRALKNKQVKKI
                     ILTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLGYYMTTRTIEIAPL
                     AYMRGRTLDNAFIILDEAQNSTDLQLKMFLTRIGANAKAIITGDMTQVDLPRNQKSGL
                     ATAVRILKNIDGIAHIELDEEDVVRHRLVKHILRAYNQEHEKENPQFRETPPIVKKDK
                     QE"
     gene            1479..1979
                     /locus_tag="PK977_18200"
     CDS             1479..1979
                     /locus_tag="PK977_18200"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRF20118.1"
                     /translation="MKDELKPIKRSKQLAPLSREHHDGLLFAWKIKQGIDNHTPAERM
                     RKYALWFWRHHMKPHFYQEEKILAPYLPDDNPMKKRLKEEHDHIRELVLGLDDETGSD
                     KRHLIILSDLLNHHIRFEERELFPFLEKLLTPEQLDKVFSELEQHPVSCGEYQDDFWV
                     KKKNET"
     gene            2115..>2883
                     /locus_tag="PK977_18205"
     CDS             2115..>2883
                     /locus_tag="PK977_18205"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF20119.1"
                     /translation="MKTNYILFSFFLLFSLASSAQLYKVSGIILDNKRERLPLASVEI
                     KELKKGTVSKDDGSFAFYLERGQYDLVVSMIGYKTKIVTFYITNQDVIENIELEPESA
                     SMSEVVVKAKYRDRAEELIKNVIKNKDVVLDAIGSYSCNAYIKAFQLDSSNTKKKSEG
                     DSVFVADFSNMSMTEILLHIDRNTAGQIKEQRHGVNKRGSTESLFYLSALDGDFFIYD
                     NLIKAPPVSKIPFVSPISYSGLMAYRFKTLKIDRTIKP"
BASE COUNT          830 a          572 c          615 g          866 t
ORIGIN      
        1 tggatgattt tgcatcaggt ttcttgtcgt cacttttgca ggctgcaaaa aaaagcagga
       61 acaaaaagaa tagattattt cctttcattc ctgcaaagta atgaatcttt tttcagcaaa
      121 cttattcctg cttatccttc tttacgatag gcggagtttc tctaaattgt gggttttctt
      181 tttcatgttc ctggttgtat gccctcagga tatgcttcac caggcgatga cgaaccacat
      241 cttcttcatc cagttcgata tgggcaatgc catcaatatt ctttaatatc ctgaccgccg
      301 tagccaagcc gcttttttga tttcttggaa gatctacctg tgtcatatca ccggtgataa
      361 tggctttcgc attggcgccg atacgggtaa ggaacatctt caactgcaga tcagtagagt
      421 tttgcgcctc atccaggatg atgaaggcat tatccaatgt tcggcctctc atataagcca
      481 ggggggcgat ctcaattgtc cgggtggtca tataataacc caacttatca gcaggaatca
      541 tatcatccag tgcatcatat aacggtcgga ggtagggatc gattttttct ttcaggtcac
      601 cgggcaaaaa accaaggctt tcaccagctt ctacagctgg cctggtgagt atgatctttt
      661 ttacttgttt attctttaat gccctcacag ccagagctac ggctgtgtat gttttacctg
      721 ttccggcagg gcctatcgca aaaacaatat cattcttttc ggcaacctgc accagtcttt
      781 tctgattggc tgttctggct cttacggatt ttccgttggg accaaatacc aatacttcat
      841 tcgggttctt ttcctggaaa tgatcaatta cttctgcatc atcacctccc agtacctgct
      901 cgaaatagtt ctggctcata tgtccgtttc tttcgaggta ggaaatgatg agagagatct
      961 tttcccttgc tgactcaatt tgttccggcg aaccacttag ttttaactgg gtgccgcggg
     1021 aaagaatttt taaaagggga aatttctttt tgaggatgtc gagtttgccg ttattgactc
     1081 cgaaaaattc gatagggtta acggtttcaa ggttgatgat agtgtctgtc aatgcacttc
     1141 aattttagtt tggttcctgt tcccattcgt ggtgaccggg ctgaaccatg tttcaccatg
     1201 ccggcgaaag gcggtaccgg atttctgttg cgtctgctgg tctttgccag cagctggccc
     1261 ttctttatca atattactca gcaatattgt tatcacctaa agttacttat tcacagatgt
     1321 gaatacaaat aaggcttatc aaattatagt taatgatttt ttatgtatac aagcggtagt
     1381 gttatgcttt ggtaaaagaa cctgctaagg tgtatcttat ccatggtggt agaatcaaca
     1441 gtactgacat tgctttcaaa aaaatgtaac tttaaagtat gaaagacgag ctaaaaccca
     1501 tcaagcgcag taaacaatta gctcctctca gccgggagca tcatgatgga ttattgtttg
     1561 cctggaaaat aaaacaggga attgacaacc atactccggc agaaagaatg cggaaatatg
     1621 cactttggtt ctggaggcat catatgaagc ctcattttta ccaggaagaa aaaatattgg
     1681 caccttatct gccggatgat aatcccatga aaaaaaggct gaaggaagaa catgaccata
     1741 tccgggaact ggtgcttggg cttgatgatg aaactggttc tgataaacgg catcttatta
     1801 ttctcagcga cctgctgaat catcatattc gttttgaaga aagagaatta tttccttttt
     1861 tagaaaaatt actgacaccg gagcaactgg ataaagtctt ttcagaattg gaacaacacc
     1921 cggtcagctg cggagagtat caggacgatt tttgggtcaa aaaaaagaat gaaacatgat
     1981 aagtgtatag atgatgggta gacatagttc ttaatttgaa gatacttgtt aaattcttgg
     2041 aaaagatggt aagccagcta agccgattca aatagttgcg caaattttgc agactgaatg
     2101 gccaatcaag gataatgaag acgaactata ttcttttctc ttttttctta ttgttttccc
     2161 tggcatcttc tgcccaatta tacaaggttt caggtattat tctcgacaat aaaagggaaa
     2221 ggctgccatt agccagtgta gaaatcaaag aacttaaaaa aggaaccgta agtaaagacg
     2281 atggctcttt tgcgttctac ctggaaagag ggcaatatga cctggtggta agtatgatcg
     2341 gttataaaac caaaatcgtc actttctata tcaccaacca ggatgtcatt gaaaatatcg
     2401 aactggaacc ggaatctgcc agtatgtctg aagtggtggt taaggctaag taccgcgaca
     2461 gggcagaaga gctaatcaaa aacgtaataa agaataaaga tgtagtgctg gatgccattg
     2521 gctcctactc ctgcaacgcc tacataaaag catttcagct tgattccagt aacacaaaga
     2581 aaaaatcaga aggcgatagt gttttcgttg cggatttcag caacatgtcg atgacggaaa
     2641 tactcctgca cattgaccgg aatacagccg ggcaaataaa ggaacagcgt catggggtaa
     2701 ataaacgggg cagcacagaa agtttgtttt acttgtctgc cttggatggt gattttttta
     2761 tttatgacaa cctgataaaa gcgccgccag tttcaaagat tccttttgtt tctcccatta
     2821 gttatagtgg cctgatggca taccggttca agactttaaa aattgacaga accatcaagc
     2881 cta
//
LOCUS       DAORLS010000359         2867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_18991_length_2867_cov_1.813656, whole genome shotgun sequence.
ACCESSION   DAORLS010000359 DAORLS010000000
VERSION     DAORLS010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18991_length_2867_cov_1.813656"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>891)
                     /locus_tag="PK977_18210"
     CDS             complement(<1..>891)
                     /locus_tag="PK977_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRF20120.1"
                     /translation="GDDNDKIIPLLHRALEKVQMIKKLENLKKQAGHRYSFDSILGDS
                     VEIKEAITTAKKVAATDATVLLLGETGTGKEVFAQAIHHNSSRSNKSFVAVNCSSFSK
                     ELLESELFGHKAGAFTGAVKDKKGLFEEANGGTIFLDEIGEMPFEIQSKLLRVLEGGE
                     FIRVGDTKPTKVNVRIIAATNQDLQKEIETGRFRSDLYYRLSVFQITLPSLREREKDI
                     RVLAAHFAELFAAKTNKPVPALNSDYLDTLQHYNWPGNIRELKNIIERSVILNEDGIL
                     SLKDLPLELRANNSSFSVFDL"
     assembly_gap    892..1044
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1045..1218)
                     /locus_tag="PK977_18215"
     CDS             complement(<1045..1218)
                     /locus_tag="PK977_18215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF20121.1"
                     /translation="MSTILIIDDEEKLRRLLKRIISLEGFDVIEAGSFKEAARLMDKE
                     VPDVVLCDVKLPDG"
     gene            complement(1376..1777)
                     /locus_tag="PK977_18220"
     CDS             complement(1376..1777)
                     /locus_tag="PK977_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20122.1"
                     /translation="MKQLILVLSVFSLAACNNSSLSKKLKGSDSLAISFYYPNTDSVV
                     QTVNTTEQKAIRRLLQFVDGKETEQYKCGYDGTMTFYIQGKLVLPVVFKYSEEGCRHF
                     LFDEDNKLRSTKMSNEAADFLKSLAVGKSWY"
     gene            1891..>2867
                     /locus_tag="PK977_18225"
     CDS             1891..>2867
                     /locus_tag="PK977_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20123.1"
                     /translation="MKTRLFILTLLMLSYCCEPARAGTGEVQSVNFDFFGNRIEIQAD
                     VSFNIPFGPERNATAISNFAQQLVKSRYETVVQSLLYYKKQLKLEDWLYYQLIRKTAQ
                     QISPKADDYYRYTLYKWFLLLQSGYDATLKAGKDKLLFYVQCDENIYNIPSYLLNGKQ
                     YVCLNYHDYGSNINFQQETFVPLNLPLITVAKSFSYKISRLPEFSPADYQEKELQFNY
                     NQHEYNFKVKLNPQIKTIFANYPVVDYASYFNIPLSHETYRSLIPLLKKNVKGMSVKY
                     GVDYLMRFTRYAFLFKQDNQQFGGEKRLSPEQTLLFEQSDCDDRAALFF"
BASE COUNT          798 a          605 c          494 g          817 t
ORIGIN      
        1 aagatcgaaa accgaaaaag aagaattatt cgccctcagt tcaagcggca ggtcctttag
       61 agacaggatg ccgtcttcat tcaaaataac cgaacgttca ataatatttt tcaattcccg
      121 gatattgccc ggccagttgt aatgctgaag tgtatcaagg taatcgctat ttaaagccgg
      181 aaccggtttg tttgttttag cggcaaaaag ttcggcaaaa tgagctgcca aaacccgaat
      241 atctttttct ctctcccgca gagaaggtaa ggtgatctga aaaacagata atcgataata
      301 taagtcagaa cgaaaacgtc cggtttctat ttccttctgc aaatcctggt ttgtagctgc
      361 aataatgcgg acatttactt tggtaggctt tgtatcaccc actctaataa actcacctcc
      421 ctccaaaacc cttagtagtt ttgactggat ttcgaaaggc atttcaccga tctcgtctaa
      481 aaaaatagta ccgccattgg cttcttcaaa taatcccttt ttatctttta cggcaccggt
      541 aaaggcacct gctttatgac cgaaaagttc gctttccagc aattctttac taaaagaact
      601 gcagttaaca gcgacaaacg atttattgct gcggctactg ttgtgatgaa tggcttgtgc
      661 aaacacttct ttacccgtcc cggtttcacc gagtaataaa acagtggcat ccgtagctgc
      721 taccttttta gctgttgtta tcgcttcttt tatttcaact gaatccccaa gtatactatc
      781 aaatgaatac ctgtgcccgg cttgtttttt cagattctcc agttttttta tcatctgaac
      841 tttttccaat gcccggtgca gcaacggaat tatcttatca ttatcatctc cnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnntccatc aggcagtttt acatcacaaa gcaccacatc
     1081 cggtacttct ttatccatta gtctggcagc ttctttgaat gaaccagctt ctataacatc
     1141 aaacccttcc aggctgataa ttcgtttaag caacctgcgt agtttttctt cgtcatctat
     1201 gatcagaatg gttgacatta cttcatctta taatatttgc taacctaaat ctggcaaaaa
     1261 ctgcccaatt acatacaaaa ctatgactct gctatataaa agttgaattt tgcctcacta
     1321 aaggcacaga catcatccag atgtactctt taaaaaaagg ccaagaatac ctgcattaat
     1381 accaactctt ccccactgcc aggcttttca aaaaatcagc cgcttcattg ctcattttag
     1441 tactcctgag cttattatct tcatcaaata aaaaatgacg gcagccttct tcgctgtatt
     1501 taaatacaac gggtaaaacc agtttgccct ggatataaaa agtcatggtg ccatcatagc
     1561 cgcatttata ctgctctgtt tccttgccat ctacaaactg cagcaggcgg cgaatagctt
     1621 tttgttctgt tgtatttact gtttgtacaa cggaatcggt attagggtag tagaaactga
     1681 tagcaagact gtcgctgcct tttagttttt tgctcaggga tgaattgttg caggcagcta
     1741 aagagaatac ggacaataca aggattaatt gtttcaagat cgttttttgg aaagatacaa
     1801 aaaatgccgg ctgataaggc ttacacctca aaagccaaaa acttaatatt actttattgt
     1861 taagtttttg taacttgacc ggaagtccat atgaagaccc ggctatttat attaaccctg
     1921 ttgatgctga gttattgctg cgagcctgcc cgggccggta caggtgaagt acaatccgtc
     1981 aactttgatt tctttggaaa ccgcatagaa atacaggcag acgtctcttt taatattcct
     2041 tttggccccg aaagaaatgc tactgctatc agcaattttg cccaacaact tgtcaaaagc
     2101 cggtatgaaa cggtagttca aagcttgctt tactacaaaa agcagttgaa gctggaagac
     2161 tggttgtatt accagctgat ccgtaaaacc gcccagcaga taagccctaa ggctgacgac
     2221 tattaccgct ataccctgta caaatggttc ctgctgctgc aatccggcta tgacgcaacc
     2281 ttaaaagccg gaaaagataa actgctcttt tatgtgcagt gtgatgaaaa tatctacaat
     2341 atcccttctt acctgctcaa tggcaaacag tatgtatgtt tgaattacca tgactatggc
     2401 agcaacataa actttcagca agaaactttt gttccgctga acctgcctct cattacggtg
     2461 gctaaatctt tttcttataa aataagccgc ttacccgaat tcagcccggc cgattaccag
     2521 gaaaaagaat tacagtttaa ctacaaccag catgagtata acttcaaagt gaagctgaac
     2581 ccgcagatca aaaccatttt tgccaattac ccggtagtgg attatgcttc ttatttcaac
     2641 ataccgctaa gtcatgaaac ttaccgttcg cttattccct tgctgaaaaa aaatgtaaaa
     2701 ggcatgagtg tgaaatatgg ggtggattac ctgatgcggt ttacccgtta tgcttttttg
     2761 tttaaacagg acaaccaaca attcggaggt gaaaaaaggc tttcacccga acaaaccttg
     2821 ttatttgagc aaagcgattg tgatgaccgg gcggcgttgt tctttta
//
LOCUS       DAORLS010000360         2858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19066_length_2858_cov_1.114520, whole genome shotgun sequence.
ACCESSION   DAORLS010000360 DAORLS010000000
VERSION     DAORLS010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19066_length_2858_cov_1.114520"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    315..467
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<468..891)
                     /locus_tag="PK977_18230"
     CDS             complement(<468..891)
                     /locus_tag="PK977_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20124.1"
                     /translation="MRMIFKSLLPAVGLILCTSCANTNNKQEQARDEIRRAEKDFEKM
                     AAEKGIAEAFSFFADTSAVIKRENDTLISGRDAIRNYYSADYFKSASVKWSPDFTDAS
                     EDGKWGYTYGKYIWQSKDSTGITHETTGVFHTVWKKQKD"
     gene            complement(914..1252)
                     /locus_tag="PK977_18235"
     CDS             complement(914..1252)
                     /locus_tag="PK977_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20125.1"
                     /translation="MNTHSPDELIQIATSLQKRGYNQEEITLQLRQQGAPENLLQEII
                     GELKKIRLLRKRNTGFACCSIGVVLLIVGCMLTLFLYDSGSSIKFAMYGLTTIGVIFS
                     MKGLVDIMGW"
     gene            complement(1311..>1837)
                     /locus_tag="PK977_18240"
     CDS             complement(1311..>1837)
                     /locus_tag="PK977_18240"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF20126.1"
                     /translation="DASDLPFENGSFDAVSCRFGFMFFPDMQLAANEIYRVLKPGGRF
                     ATTVWNFPEKNFWVTAIMGTIMRLMEIPPPPPEVPGMFRCCKPGRMKDILEKAGFSDI
                     EEREILSTLKTKSVDQYWEMMTEIGASVVAALSQADDGMRSKIKEEVFLLLKQKCPEI
                     VALEASAIIITARK"
     assembly_gap    1838..1975
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1976..2173)
                     /locus_tag="PK977_18245"
     CDS             complement(<1976..2173)
                     /locus_tag="PK977_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HRF20127.1"
                     /translation="MNKDLEAIKEQQKASWNRFAAGWKKWDPVTMEFLQPMKEAIINY
                     LQPKDGQKILDIAAGTGEPALS"
     assembly_gap    2233..2273
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2274..2714)
                     /locus_tag="PK977_18250"
     CDS             complement(<2274..2714)
                     /locus_tag="PK977_18250"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF20128.1"
                     /translation="MIRLAVQIDIPDLVPLINSAYRGEASKKGWTTEANLISGDIRTD
                     KNQMEELMKTPGAVFLVYQNETGTLEGSVFLQKKENKLYLGMLSVLPELQAKGIGKKL
                     MSAAEAYAILENCNSIYMRVISARHELIAWYEKLGYKNSGKTEPF"
BASE COUNT          678 a          551 c          484 g          813 t
ORIGIN      
        1 acaggaacgt aagcgaatgg gagggaaaca aaaggagact cctgagtctt aacctgtatc
       61 aatgagtata ttttaaagaa agaagatatt cacaattttg cagaatctta tccgccactt
      121 gtcaagtggc attttttttg tcaattatgt gcggccatct gcaatatatt caggtctcac
      181 caacccggtt aagaatatga ttagtaatga aagaccaaag cccgtttttt gtaagtccca
      241 acccggtgcc cgttaatgct tctataaaca tacagttctc cggtgtacca tttaatcccg
      301 atttctattg catcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt ccttttgttt
      481 tttccaaacg gtatgaaaga cgcctgttgt ttcgtgggta atacctgtac tgtcttttga
      541 ttgccatata tacttgccat aggtataccc ccatttgccg tcttcagagg catccgtaaa
      601 gtccggtgac cattttacgg atgctgattt gaaatagtca gctgaataat aattccggat
      661 agcgtctctt ccggagataa gggtatcatt ttctcttttg attacagcgg atgtatctgc
      721 aaaaaaggaa aaagcctccg ctataccttt ttcggcagcc attttttcaa agtctttttc
      781 tgccctgcgg atttcatccc gggcttgctc ctgcttgttg tttgtattag cacaagaagt
      841 gcaaagaatt aaaccaactg ccggcaacag gcttttgaaa atcatacgca tagaaaagat
      901 tgaaatactg gtattaccag cccataatat caacaaggcc tttcatggag aaaatcacac
      961 cgatggtagt caaaccatac atggcaaatt tgatactaga tccgctgtcg taaagaaaaa
     1021 gggtgagcat acagccaact atgagcagga ctacaccaat actgcaacag gcaaaaccag
     1081 tatttctttt gcgtagcagc ctgatttttt tcaattctcc tataatctcc tgtaaaagat
     1141 tttcaggagc accctgctga cggagctgca aagtaatttc ttcctggttg tatcctcttt
     1201 tctgtaaaga agtggcaatt tgtattaatt catccggaga atgagtattc atacaacaag
     1261 cttttcgtta agatacaaac tttttattcg gtaaaaatta ttttgaacct ttattttctg
     1321 gctgtaataa tgattgcact tgcttccagg gctactattt caggacattt ttgtttgaga
     1381 agcaggaaaa cttcttcttt aatttttgaa cgcataccat cgtcggcctg gctaagggca
     1441 gcaacaacag aagccccgat ttctgtcatc atttcccaat actggtcaac tgattttgtt
     1501 tttagagttg agagaatctc tctttcttca atgtcactga atcctgcctt ttccagtatg
     1561 tctttcattc tgcccggttt acagcaacgg aacataccgg gcacctccgg aggtggcggt
     1621 ggtatttcca tcagccgcat gattgttccc attatggctg ttacccagaa attcttttcc
     1681 ggaaagttcc agactgttgt ggcaaatcga ccacccggtt tgagtacccg gtaaatttca
     1741 ttagccgcca attgcatgtc aggaaaaaac ataaatccaa accggcaact cactgcatca
     1801 aaactgccgt tttcaaaagg gaggtcagat gcatcgcnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactta
     1981 aagccggttc acctgtgccg gcggctatat ccagtatttt ttggccatct tttggttgta
     2041 aataattgat gatggcttct ttcattggtt gcaggaattc cattgtgacc ggatcccatt
     2101 ttttccagcc tgcggcaaac ctgttccatg atgccttttg ctgttccttt attgcttcta
     2161 aatccttgtt catttgtaat tatggatcgg tttagatcat tttttccatg atgatgaatt
     2221 caataggcct gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagggt
     2281 tctgtttttc ctgaattctt ataacccaat ttttcatacc aggctatcag ttcatgcctt
     2341 gcagaaatga cccgcatata aatgctgtta caattttcca gtatggcata agcctctgct
     2401 gcactcatta attttttccc aatacctttt gcctgtagct caggcaaaac actcagcatg
     2461 cccaggtaaa gtttattttc tttcttttga aggaaaacag atccttccag cgtaccagtt
     2521 tcattttggt aaacaagaaa tacagcgccg ggtgttttca tcagttcttc catttggttt
     2581 ttatcggtac gaatatcgcc gcttataaga ttagcctctg ttgtccagcc tttctttgaa
     2641 gcttcaccac ggtaagcact attgatcagt ggcacaaggt caggtatgtc gatttgcaca
     2701 gccaatcgta tcatataaca attttattgg attgaaatta agcattgaat gatttcagag
     2761 cataaaaaaa ccgttccgcc tgggcggaac ggtttatagt gtacaacgta ttgtcagaat
     2821 ctcggtctgt ttctgtcacg atcttcaaat cggtccca
//
LOCUS       DAORLS010000361         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19216_length_2841_cov_2.235822, whole genome shotgun sequence.
ACCESSION   DAORLS010000361 DAORLS010000000
VERSION     DAORLS010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19216_length_2841_cov_2.235822"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>822)
                     /locus_tag="PK977_18255"
     CDS             complement(<1..>822)
                     /locus_tag="PK977_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HRF20129.1"
                     /translation="VKKALAFKLNPFGEKSLGANKRIGCLFLNPSMRTRLSTQIAAQN
                     LGMEAIIFNVGSEGWALEFEEEAIMSGTTVEHVKDAAPIFGKYFDILAIRTFPSLKNR
                     EDDYSELYIKQFIKYAGIPVVSLESATLHPLQSLTDVITISESFKDTRKPKIVLTWAP
                     HVKPLPQCVANSFSQWINAWGKAEFIITHPEDYNLSEEFTKRAIITTNQDEALKDADF
                     VYVKNWSTFNDYGKIYESDPKWMLTNKKLEQTNNAKVMHCLPVRRNVELSDEVLDG"
     assembly_gap    823..850
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(903..2048)
                     /locus_tag="PK977_18260"
     CDS             complement(903..2048)
                     /locus_tag="PK977_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF20130.1"
                     /translation="MNLFDVYPINEITIVKAKGSYVWDDKGEQYLDLYGGHAVISIGH
                     THPHWVQRIEEQLEKIAFYSNSIRIPLQQQLAEKLGKVSGKEDYQLFLCNSGAEANEN
                     ALKLASFHTGRKKVIAFSKSFHGRTSLAVAATDNKNYVAPVNETENVIFLPFNDDTAL
                     ERCFDENGKEIAAVIIEGIQGVGGINVADDSFLRKIRSLSDEYEAVYIADSVQCGYGR
                     SGKFFSHDFAGVNADIYTMAKGMGNGFPVAGIIIAPHIKPKHFQLGTTFGGNHLACAA
                     ALAVLEIIEEEKLMGNAVMIGKYLKDELESIPQLKNVRGRGLMIGFDVPEDLKDLKKN
                     LLWNQKIFTGEAKPNVIRLLPSLALKKRDAEQFIESIKEEIGLLETV"
     gene            complement(2050..>2841)
                     /gene="argC"
                     /locus_tag="PK977_18265"
     CDS             complement(2050..>2841)
                     /gene="argC"
                     /locus_tag="PK977_18265"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRF20131.1"
                     /translation="NDNIDVLFLCLGHGESKKFLTENKIADRIKIIDLANDFRLTMQS
                     EIANRKFIYGLPELNRDKIKTAANIANPGCFATSIQLGLLPLAKAGLLTDIYTTGITG
                     STGAGQSLSSTSHFSWRANNIQAYKTLTHQHLGEIGESLLHLQPKNDIELSFVPWRGD
                     FTRGIFVSSTLHCDLSLEELNKIFEDYYSTHPFVQVSKDAIFLKQVVNTNKAVIQLEK
                     AGSKLVVHSAIDNLLKGASGQAVQNMNLLFGLDETSGLKLKANYF"
BASE COUNT          752 a          584 c          536 g          941 t
ORIGIN      
        1 ccatccaaaa cttcgtcact caattcaaca tttcttctta caggcaggca gtgcattact
       61 ttcgcattat tggtctgttc taatttttta tttgtaagca tccatttagg gtcactttca
      121 tagatcttgc catagtcatt aaaagtgctc cagtttttta cgtaaacaaa atctgcatct
      181 ttaagtgctt catcctggtt ggtagtaata attgctcttt tagtgaactc ttcacttaag
      241 ttataatcct caggatgagt gatgataaat tctgccttac cccatgcatt gatccattgg
      301 gaaaaactgt tagctacaca ttgtggcagt ggttttacat gcggggccca ggtcaataca
      361 atttttggct ttcttgtatc tttaaaagat tcagaaattg taattacatc agttaaactt
      421 tgtaaaggat gaagcgttgc gctttccagg ctaactaccg gtatgcctgc atatttgatg
      481 aattgcttaa tatataattc gctgtagtcg tcttcccggt tctttaatga tggaaatgtt
      541 ctgatggcaa gaatgtcaaa atatttacca aaaatgggag cagcgtcttt tacatgctct
      601 actgttgtac cgctcatgat ggcttcttct tcaaattcga gggcccagcc ttcgctgccg
      661 acattaaaaa taatggcttc catgcccagg ttctgtgctg caatctgggt gctgagcctt
      721 gtccgcatgc tggggttcag gaataagcaa ccaatgcgtt tattggcgcc caatgatttt
      781 tcgccaaacg ggttaagctt gaacgctaat gcttttttta cannnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn cgtcaatatc agttacatca tgaacggaaa caaaattttt catttttatt
      901 tattagacag tttcaaggag accaatttct tctttaatac tttcaataaa ttgttccgca
      961 tctcttttct tcaaggccag tgaaggaagg aggcggataa catttggctt tgcttcaccg
     1021 gtgaatatct tttggttcca aagcaggttt ttcttcaggt ctttcaagtc ttccggaaca
     1081 tcaaaaccga tcataagacc ccggcctctt acgtttttta gttgtggaat agactctaat
     1141 tcatctttca ggtatttgcc aatcattacg gcattaccca tcagtttttc ttcttcaatt
     1201 atttccaata cagctaaagc agctgcgcaa gcaagatgat ttccgccaaa tgttgtaccc
     1261 agctgaaaat gttttggctt gatatgcggg gcaatgataa tgccagcaac agggaagcca
     1321 ttacccattc ctttcgccat agtgtatata tctgcattta cccctgcaaa gtcatggctg
     1381 aagaatttac cgcttctgcc atagccgcac tgcacactgt cagcgatata aacggcttca
     1441 tactcatcgg atagggaacg gatttttctg agaaatgaat catcagctac atttataccg
     1501 ccaactccct gtataccttc aattattact gcagcaattt cttttccgtt ctcatcaaaa
     1561 cagcgttcaa gtgctgtatc atcgttgaaa ggaagaaata ttacgttttc tgtttcattg
     1621 acaggcgcaa cgtagttttt attatcagta gcggctactg ccagggaggt tcttccatga
     1681 aaggatttgc tgaatgcaat cacttttttt ctgcctgtat gaaatgaagc cagtttcagg
     1741 gcattttcat tggcttcggc accgctgtta caaagaaaaa gctggtagtc ctctttgccg
     1801 gaaacttttc ccagtttttc tgccagttgt tgctgcaaag gaattcttat ggagttggaa
     1861 tagaaggcaa ttttttccag ctgctcttca attcgctgca cccagtgtgg atgtgtatgg
     1921 cctatgctga ttactgcatg gccgccgtaa aggtcaaggt actgttcgcc tttgtcatcc
     1981 catacataag agccctttgc ttttacgata gtgatttcat taatcgggta tacgtcaaaa
     2041 agattcatgt taaaaataat tagctttaag tttcaaaccg ctggtttcat ctaacccgaa
     2101 caacaggttc atattttgta cggcttgtcc actggcgcct tttaataaat tatcaatggc
     2161 tgaatgaact accagtttac tccctgcttt ttccagttgt ataacggctt tattagtatt
     2221 cactacctgt tttaaaaaga ttgcatcttt gcttacctgc acaaagggat gagtgctata
     2281 ataatcttca aatattttat ttaattcttc caggcttaaa tcgcagtgta atgtcgagct
     2341 gacaaaaatc cctcttgtaa aatcacccct ccagggtaca aagctgagct caatatcgtt
     2401 tttgggttgc aggtgcaaaa gcgattcgcc tatttctccg agatgctggt gagtaagtgt
     2461 tttataagcc tgaatattat tggctctcca gctaaaatgt gatgttgacg aaagggattg
     2521 acctgctcct gtagaacctg taattcctgt agtataaata tcagttaata aaccagcctt
     2581 ggctaaaggt aaaagcccca gttgaattga tgtagcaaaa caacccggat tggcaatatt
     2641 agcagctgtc tttattttgt ctctgttcag ttcaggaaga ccataaatga atttacgatt
     2701 tgcgatctca gattgcatag ttagtctgaa atcatttgcc aggtctatga tttttattct
     2761 gtcagcgatt ttattttctg tcaggaattt ttttgactct ccatgcccaa ggcaaagaaa
     2821 caatacatca atgttgtcat t
//
LOCUS       DAORLS010000362         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19217_length_2841_cov_1.907753, whole genome shotgun sequence.
ACCESSION   DAORLS010000362 DAORLS010000000
VERSION     DAORLS010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19217_length_2841_cov_1.907753"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>312)
                     /locus_tag="PK977_18270"
     CDS             complement(<1..>312)
                     /locus_tag="PK977_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20132.1"
                     /translation="LSLSIKGSDKKINLDRYNVWVNEVPVFGRTGKSVRNQKTKSISA
                     TVELTLSEGMNRIEVSVMNMAGTESYRMPLYVNYSPAVKQKEMTRFIGIGIDQFKQSN
                     Y"
     assembly_gap    313..336
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<337..>671)
                     /locus_tag="PK977_18275"
     CDS             complement(<337..>671)
                     /locus_tag="PK977_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20133.1"
                     /translation="QLINNKTRDTVSFIKIDTLDWITVLKNGYYMASRNAAKTISYVS
                     GMDVYPFEQFDLQYNRPDKVLAAIGKASPTLIEAYHKAYLKRIKRAGIDSSQFLNELE
                     VPLAEITNQ"
     assembly_gap    672..685
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<686..>1019)
                     /locus_tag="PK977_18280"
     CDS             complement(<686..>1019)
                     /locus_tag="PK977_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20134.1"
                     /translation="QVADQLRRYPFYNQPPYYGYNLVWDLTTGGIKSTQAAAEFSPDH
                     RTQSKDKNYSLRNLMYETGIQTNMTLINMMRQPVDGVSEETMIILRRYYDTSSNFYKR
                     VFAGRLNGP"
     assembly_gap    1020..1037
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1038..2560)
                     /locus_tag="PK977_18285"
     CDS             complement(<1038..2560)
                     /locus_tag="PK977_18285"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF20135.1"
                     /translation="MNRITKELSIPLFLLMFSLWVFLLVCPLQTAGQPPKPALKIPTG
                     HTAQINTLAFSPDGKWIASGSTDNSLIIWDARTGFDIRKINTGEPVWYISFPEDGSGV
                     ITVSGQVGAEYSGGKSTLKKWDLATGKLLGQYSYSGSPELVPLPSQGGFLIAENSSYL
                     QKTTEQTRNMDLTDPNAVNKLDYSKMMNIDSMTKEFTKKMEELSKNGSKLDPNNKKAM
                     EEYQRKLAEIYMGSYSPVNNVLKKTIGILSSSTLKMTGKLNRAFEKAYLISYHGNEYI
                     ISPEMKMMPAAGESRFRFEAWDLKELMTANSNQSATPWKTYLVKSNNSPVYTSRSGGF
                     FAMPAQRNSMVQLWQLENTTALAEFKVNGKVLHGVEFSPNGNQLYIFSQTPDNDKKQF
                     YVEGWNTHTFKRDLFITMPTNYQTGTPRVAPSGLYYINTVAYPGWTIKQFNWKGDSTG
                     VLGGRAGAPSYFGFDGEDATVYLNFLGFPDMKEFLLAGITASVEHEAFSKGIKLTKA"
BASE COUNT          632 a          674 c          641 g          838 t
ORIGIN      
        1 tgtaattgct ttgtttgaac tggtcaatac caataccgat aaacctcgtc atctcttttt
       61 gcttcaccgc cgggctatag ttcacataga gcggcatcct gtagctttcc gtacctgcca
      121 tattcatcac agagacttcg atacggttca tcccttctga aagtgtgagc tccactgtgg
      181 cggatattga tttggtcttt tgattcctga ccgatttccc ggtcctgccg aaaacgggta
      241 cctcattgac ccagacatta taccggtcga gattgatctt cttatccgat ccttttatgg
      301 acaggctcag ttnnnnnnnn nnnnnnnnnn nnnnnncctg gttggttatt tccgccagcg
      361 gcacttccag ttcattcagg aactggctgc tgtcgatccc cgcccttttg atccttttca
      421 gataagcttt gtgataagct tcgatcaggg tggggctggc tttcccgatt gccgccagca
      481 ctttgtccgg acggttatac tgcaggtcaa attgttcaaa gggataaaca tccatacccg
      541 atacataact gatcgttttg gccgcattcc gggaagccat ataatatccg tttttcagta
      601 ccgtaatcca gtcgagggta tcgatcttaa taaaggaaac cgtatctctg gttttgttat
      661 tgatcagttg tnnnnnnnnn nnnnnggccc gttcagtcgt cctgcaaaaa cccttttata
      721 aaagttgctg ctggtatcgt aatatcttct caggatgatc atggtttcct ctgaaacacc
      781 atcaaccggt tgtctcatca tgttgatgag ggtcatattg gtttgaatgc ctgtttcgta
      841 catcaggttc cggaggctgt aatttttatc ttttgattgt gtccggtgat cgggggagaa
      901 ctcagcggcg gcctgcgttg acttgatacc accggtggta agatcccaga ccaggttata
      961 tccataatag ggtggctggt tataaaaggg atacctcctg agctgatcgg caacctgttn
     1021 nnnnnnnnnn nnnnnnntcg gcctttgtga gttttatccc cttactaaag gcttcgtgtt
     1081 ccaccgaagc agttattccg gccaataaga attctttcat atccggaaag cccaggaaat
     1141 tcaggtacac agttgcgtcc tccccgtcga aaccgaaata ggaaggcgct cccgcccgtc
     1201 cgcccaggac tcctgtacta tctcctttcc agttgaattg tttgatggtc catccgggat
     1261 aggctactgt attgatataa tacaggcctg atggtgccac cctcggtgtg cctgtctgat
     1321 aattggtggg catggtaata aaaagatccc ttttaaacgt atgggtattc catccttcca
     1381 cataaaattg cttcttatcg ttatccgggg tctgactgaa aatataaagt tgattgccat
     1441 ttggggagaa ctcaaccccg tgcagaacct ttccattgac cttaaactcg gccagtgccg
     1501 ttgtgttttc cagctgccag agttgtacca tactgttcct ttgtgccggc atggcaaaaa
     1561 atcctccgga cctggaagta taaaccgggc tgttgtttga tttgaccaga taggttttcc
     1621 agggagtcgc agactggttt gaatttgccg tcattaactc tttaagatcc caagcttcaa
     1681 agcggaacct tgattccccg gcagcaggca tcatcttcat ttccggcgaa ataatgtatt
     1741 catttccatg ataactgatg agatacgctt tttcaaaggc ccggttcagt ttgccggtca
     1801 ttttcagggt actgctgctc agaataccaa tcgttttttt cagcacattg ttaaccgggc
     1861 tataggatcc catatagatt tcagcgagtt tccgctggta ttcttccatg gcctttttat
     1921 tattgggatc cagcttgctg ccgttcttgc ttaactcctc catttttttg gtgaactctt
     1981 ttgtcatcga gtcaatattc atcattttgg aatagtccag cttgttaact gcattcggat
     2041 ccgtcaggtc catgttcctg gtctgttccg ttgttttctg gagatagctg ctgttttcgg
     2101 caatcagaaa accgccttgt gaaggaaggg gcaccagctc gggtgacccg gaataactgt
     2161 actgacccag cagcttgcca gtggccaggt cccatttttt aagcgttgac tttccgccgg
     2221 aatattccgc acccacctgg cccgaaacgg ttataacacc ggatccatct tccggaaaac
     2281 tgatatacca aacaggctca ccggtattta ttttccggat atcaaatccc gtacgggcat
     2341 cccagatgat gaggctgtta tcggtactgc cactggcgat ccatttccca tcgggagaaa
     2401 aggcgagggt attgatctgg gccgtatgac cggttggtat ttttaaagcc ggtttagggg
     2461 gctgaccagc cgtttgaagg gggcagacta aaaggaaaac ccaaagtgag aacattagca
     2521 gaaacaaggg aattgaaagt tctttggtga ttcggttcat aaaactgctt tacttattgt
     2581 tggtgtttct ttgaaagttc gggaaccgat atccatatac ttactgatac gggtgatata
     2641 attccccgga ccggtctatc cggcatccct ccaggccgga tcctttacca gaggttccag
     2701 tccatagaca ggatctcatt ccggctcgtc ggctgttgta atccattggt cagctgcagc
     2761 acacgttccc ccacaaagcg gcgaaactca ctgatcttta tcgtcgggta ttggttcatc
     2821 gcttccagca ggctgtaggt a
//
LOCUS       DAORLS010000363         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19334_length_2830_cov_1.520360, whole genome shotgun sequence.
ACCESSION   DAORLS010000363 DAORLS010000000
VERSION     DAORLS010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19334_length_2830_cov_1.520360"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>701
                     /locus_tag="PK977_18290"
     CDS             <1..>701
                     /locus_tag="PK977_18290"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218468.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRF20136.1"
                     /translation="EKHTSIDDAINAAMHYRREGEAKSIGVLCNAVDLLDRLIQRDVI
                     PDTLTDQTSAHDPLIGYVPHTLTNEQANVLREQNPDEYIRRSYESMHLHVQLMLQLMD
                     KGAITFDYGNNIRARAQEFEQQHPEASMEDLKTKGRCFDFPGFVPAYIRPLFCEGKGP
                     FRWAALSGDPADIAVTDELIMNMFPENKGMIRWMQMAKEKIAFQGLPARICWLGQGER
                     EKAGLAFNELVRTGK"
     assembly_gap    702..754
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <755..1075
                     /locus_tag="PK977_18295"
     CDS             <755..1075
                     /locus_tag="PK977_18295"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218468.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRF20137.1"
                     /translation="ASPNRETESMLDGSDAVADWPILNALVNTAGGASWVSLHHGGGV
                     GMGYSIHAGMVIVADGTADAEARLKRVLRNDPGMGVIRHADAGYDIARETLKQNDLDF
                     KDRL"
     gene            complement(1178..2086)
                     /locus_tag="PK977_18300"
     CDS             complement(1178..2086)
                     /locus_tag="PK977_18300"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF20138.1"
                     /translation="MNSKQEARLNMYDAVLAHCDANAAIVATVPAFQTAINNFEDIVD
                     DLRQAAQDEIIATTGYALDKAEQRKNLSQLTLDMSGAIYAYASSVNNLVLREQVHITP
                     SDLLRLKDELLGPACSNIVDAANANAAALVPFGVTAAKITALEDAIDDYISAVPAPRN
                     AVANRASIAESIRQYFKSADELLKTQIDKLAVQFQSSHPNFYNAYRSNRVILDPASSA
                     TQVSGTVTDQWTNNPVADVIIQVVDQAYSTTTNSDGEYILKIPVPGEYNITFSKTGYV
                     TKTEEDVEVTLGGNTSLDIILNQIPA"
     assembly_gap    2145..2162
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2454..2493
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2494..2687
                     /locus_tag="PK977_18305"
     CDS             <2494..2687
                     /locus_tag="PK977_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20139.1"
                     /translation="TDWFELLTEGVEFVTASLEMLTDGFELYENVLVMGNSLFSCLIS
                     SLRSCSSRRITSVRKAGML"
BASE COUNT          723 a          586 c          718 g          692 t
ORIGIN      
        1 atgaaaaaca taccagtatt gatgatgcca tcaatgctgc catgcattac cgccgcgaag
       61 gcgaagccaa aagcattggt gtgctctgca atgcggttga cctgctcgac cgcctgatac
      121 aaagagatgt gattcccgat acacttaccg accagaccag tgcccacgac ccgctgatcg
      181 gttatgtgcc gcatacgctg accaacgagc aggccaatgt actgagagaa caaaaccccg
      241 atgagtatat ccgccgcagt tatgaaagta tgcacctgca tgtgcagctc atgctgcaac
      301 tgatggacaa aggcgccatc acatttgact atggtaataa tatccgtgca agggcacagg
      361 agtttgaaca gcagcatccc gaagccagta tggaagacct gaaaacaaaa ggccgctgct
      421 tcgattttcc cggttttgtt cctgcctata tccgtccgct gttttgcgaa ggcaaaggcc
      481 cgttccgctg ggcggcgctg agcggcgacc ctgcggatat tgcggtcacc gatgaactca
      541 tcatgaatat gtttcccgaa aacaaaggca tgatccgctg gatgcagatg gccaaagaaa
      601 aaattgcctt ccagggattg cccgcaagaa tatgctggct gggccagggc gaaagagaaa
      661 aagcaggcct tgcttttaat gaactggtac gcaccgggaa annnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcctca cccaaccggg aaacagaatc
      781 catgctcgat ggcagtgatg ccgtggccga ctggcccatc ttaaatgcac tggtaaatac
      841 agcaggcggc gccagctggg tttccctgca ccatggcggc ggggtaggta tggggtacag
      901 catacatgcc ggaatggtta tcgtggccga cggaacagcc gatgcagaag caagactgaa
      961 acgggtattg cgcaacgacc ccggtatggg agtgatacgg catgctgacg ccggttatga
     1021 tattgcgagg gagacgctga agcaaaatga tttggatttc aaagacagat tataaaaaaa
     1081 agtagtgaaa gaatagacta aggctatagc atactattgc ggaactacaa gtgccgcaaa
     1141 gcgaataaga aagccgcctg agcaggcggc ttttgtttta tgcgggaatt tgattcagga
     1201 ttatatctaa ggaagtgttg ccacccaagg tgacctctac atcttcttct gttttggtga
     1261 catagcctgt tttgctaaag gtgatattat actctccggg cactggtatc tttaaaatat
     1321 attctccgtc tgaattggtg gtggtgctgt atgcctgatc gactacctgg ataatgacat
     1381 ctgcaacagg attattcgtc cactggtcgg tgacagtacc ggaaacctgt gtggctgatg
     1441 atgcaggatc gagaataacc cgattactac ggtatgcatt atagaaattg ggatgcgaag
     1501 actgaaactg cacagccagt ttatcaatct gcgtttttaa taactcatcg gctgatttga
     1561 aatactgacg gatagactca gcaatggatg ccctgttggc cactgcattg cgaggggcgg
     1621 gcaccgcaga tatgtaatca tcgatagcat cttccagcgc agtgatcttt gcagcagtaa
     1681 cgccgaaggg aacgagggct gctgcatttg cgttggcggc atcgacaata ttggaacagg
     1741 ccgggcccaa taattcatct ttgaggcgaa gcaaatcgct gggggtgatg tgtacctgct
     1801 ctctcagcac cagattattt acagaggaag cgtaagcata aatagcgccg gacatatcca
     1861 gcgtaagctg tgataaattt ttgcgctgct ctgctttatc cagcgcatag ccggtggtag
     1921 caatgatctc atcctgtgct gcctgcctga gatcgtctac aatgtcttca aaattgttga
     1981 tagctgtctg aaatgcggga actgtggcta cgatggctgc attggcatcg caatgtgcca
     2041 ggacggcatc atacatattg agccgtgcct cttgtttact attcatggat taaggttttg
     2101 ggtgaaagaa taagtattac ttgttgaaat tagtatcact gctannnnnn nnnnnnnnnn
     2161 nncaatacaa cttttgtggt attttttgta actgcgccta ttgcaggccg gtagcctgtt
     2221 tctgacagcg gaaaagaggg ttttgaaagg caatgaaggc caaataattt tcagttgttg
     2281 ctccctgaat tgtgatagtt gtggtttgaa ctgctgacag aagaggttga attggtgaca
     2341 gcaccgattg aactgccgat agactcgttt gaaattgcaa cagtatgtat tgaactcctg
     2401 acagactggt ttgaataacg aacagacctc tttgaacaac cagcagtatg cttnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgacaga ctggtttgaa cttctgacag
     2521 aaggtgttga atttgtgaca gcatcgcttg aaatgctgac agatggcttt gaactttatg
     2581 aaaatgtgtt agttatgggt aattccttgt tttcctgcct gatttcttct ttacgaagct
     2641 gctccagccg gaggataact tcggtgagga aagcggggat gttataaatg ctggtatgga
     2701 caaggcggcc tatgcgggtt ctgctattgc ggggaggtct ttaagaaata gtaatcttaa
     2761 ttaccgggac ctgttttgct gccaagtggt ataattggcg attagagcag ccagtcctgg
     2821 cgcaggacta
//
LOCUS       DAORLS010000364         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19419_length_2820_cov_1.717179, whole genome shotgun sequence.
ACCESSION   DAORLS010000364 DAORLS010000000
VERSION     DAORLS010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19419_length_2820_cov_1.717179"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..333)
                     /locus_tag="PK977_18310"
     CDS             complement(163..333)
                     /locus_tag="PK977_18310"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20140.1"
                     /translation="MDGEYGQKDICLGVPVIIGKNGWEKIIDFKLNESEQAAFNKSAE
                     AVRSMNDVLKTL"
     assembly_gap    338..424
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<425..1091)
                     /locus_tag="PK977_18315"
     CDS             complement(<425..1091)
                     /locus_tag="PK977_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRF20141.1"
                     /translation="MKVTVVGAGAVGATCADNIARKELCNELVLLDIKEGIAEGKAQD
                     MMQTATLLGFDTKITGSTNDYTKTANSDVVVITSGLPRKPGMTREELIGVNAGIVKGV
                     CENILKHSPNAIIIVISNPMDTMTYLALQSTGLPKNRIIGMGGTLDSSRFKYQLSQHL
                     GCSPSDLNAVVVGGHGDTTMIPLIRLATWNSTPVTNFLSAEQQEKIVKDTMVGGATLT
                     ALIG"
     gene            complement(1186..>1377)
                     /locus_tag="PK977_18320"
     CDS             complement(1186..>1377)
                     /locus_tag="PK977_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRF20142.1"
                     /translation="FMEKYKLPAYENIIVAQDYTFFLPPYFQFNNLPFLAFYNKKKKL
                     VSTFGGSLPVDKIVAELRK"
     assembly_gap    1378..1396
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1397..1717)
                     /locus_tag="PK977_18325"
     CDS             complement(<1397..1717)
                     /locus_tag="PK977_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRF20143.1"
                     /translation="MKKYSFLLALLVLSFAAFSQHNHQLDTVPAPATRFKSFPPVKLL
                     MPDSSTYFTKDVLPKKTPVLLMVFHPSCEHCQQETENITKNIEKFKGIQIVMSTMVPL
                     FEMKA"
     gene            1839..2405
                     /gene="efp"
                     /locus_tag="PK977_18330"
     CDS             1839..2405
                     /gene="efp"
                     /locus_tag="PK977_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRF20144.1"
                     /translation="MANTADISRGMILNLDGSLYSVVEFGENKTARAAAKVWAKLKGV
                     DNSRTIEKTWNSGDTIYPVRVERRYYQFLYKDDTGYNFMDNETFEQIALQEATIDAPQ
                     YLKEGQEVGVLVNTENDQVLGAELPDKIVLQVTYTEPGLKGDTATRTLKPATLETGAS
                     IGVPLFVNEGELIRVNTKTGEYVERVKE"
     assembly_gap    2492..2600
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2609..>2820
                     /locus_tag="PK977_18335"
     CDS             2609..>2820
                     /locus_tag="PK977_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HRF20145.1"
                     /translation="MDFKQIQELIKMINKSNIGELTIEQKDFKVTIKQKEDHVTQVVA
                     PAAVHAAPVHHAPAAAPSAPAAPAAE"
BASE COUNT          764 a          527 c          577 g          737 t
ORIGIN      
        1 gtactcgaga aagaaaaaat tgctgctgct gatatcaagg atatcacgaa ggctactact
       61 ccgtttgatt ttgaagggaa gttgtggagg tggtgttatc tcatcaataa atagaaaaag
      121 ctcttcattt tgaagagctt tttttataca atcaaaaatt tattacaacg tcttcaatac
      181 atcattcata cttctcaccg cctctgcact tttgttgaaa gcggcttgtt cgctttcgtt
      241 gagtttgaaa tcgatgattt tttcccaccc gtttttgcca atgataacag gtacgccgag
      301 acaaatatct ttttggccat actctccatc caatgctnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnntgccga tcaatgctgt taatgtagcg ccacccacca tggtatcttt tacaattttc
      481 tcctgttgtt cggcagaaag gaagttggta accggtgtgc tgttccaggt agcgaggcgg
      541 atcaaaggaa tcatggtagt atcaccatgg ccgccaacaa ccactgcatt caaatcagaa
      601 ggtgaacaac caagatgctg gcttaactgg tatttgaagc ggctgctgtc taatgtaccg
      661 cccataccga tgattctgtt tttaggtaaa ccggtggatt gtaaagcgag ataggtcatc
      721 gtatccatcg ggttgctgat aacgatgatg atggcattgg gagaatgttt cagaatgttt
      781 tcgcaaacac cttttacaat accggcgtta acgccaatca attcttcacg ggtcataccg
      841 ggttttcggg gcagaccaga agtgatgacc actacatcac tgttagcggt tttggtgtaa
      901 tcatttgtac tgccggtgat cttggtatca aaccccagca gggtagcggt ctgcatcata
      961 tcctgggctt tgccttctgc aataccttct ttgatatcga gcaggaccag ttcattacat
     1021 aattccttac gggcaatatt atcagcacag gtagcaccta ctgcgccagc gccaactaca
     1081 gttactttca tgatagttta tttaagattg aaaattgaat tattacgtag tgtaacaggc
     1141 cgcaaaggta aaggttgccg ttattttatg atggtgatga ctgagttatt ttcttagttc
     1201 tgccactatt ttatcaaccg gtagtgagcc gccaaaggtg gacaccagtt ttttcttctt
     1261 gttataaaag gccaggaaag gcaggttgtt gaactgaaaa taaggtggca ggaagaaagt
     1321 ataatcttgt gccacaataa tgttttcata agcgggcagt ttgtatttct ccataaannn
     1381 nnnnnnnnnn nnnnnnggcc ttcatttcaa acaaaggcac catggtggac attacgatct
     1441 gaatcccctt gaacttttct atgtttttag taatgttttc ggtttcttgc tggcaatgct
     1501 cgcaggaagg atgaaacacc atcagtaata ccggtgtttt tttgggtaaa acatcttttg
     1561 taaaatatgt gctgctatcg ggcatcagca gttttacagg cggaaaactt ttgaaccggg
     1621 tggcaggggc tggtacggta tctagctggt gattgtgctg gctgaaagca gcgaatgaaa
     1681 gaaccagtaa tgccaataaa aaggagtatt tcttcatttg tcaaagaaaa gatattctct
     1741 gcaagattta ctacccgcag ttgggtaata atagtagacc agattccctt tgtcatcagg
     1801 cattgtaacc ttatctttgc cgcctcaaaa tttaaaaaat ggcaaataca gcagatatca
     1861 gccgtggcat gatcctgaac ctggatggca gcctttattc cgtagtagaa tttggcgaaa
     1921 acaagactgc ccgtgcagca gccaaagtat gggctaaact aaaaggggtc gacaacagcc
     1981 gtaccattga aaaaacatgg aactccggcg acactattta cccggtgagg gtggaaagac
     2041 gttattacca gttcctatat aaagatgata ccggttataa cttcatggat aatgagacct
     2101 ttgaacagat tgcactgcaa gaggctacta tcgatgctcc acaatacctt aaagaaggac
     2161 aagaagtagg agtactggta aatacagaaa atgaccaggt attaggtgct gaacttcctg
     2221 ataaaattgt attacaggtt acctacactg agccaggtct aaaaggtgat actgctaccc
     2281 gtaccttaaa gccggctacc ttggaaacag gcgctagcat tggcgttcct ttatttgtga
     2341 atgaaggaga actgatccgt gtaaatacga agacaggaga gtatgttgaa agagtgaaag
     2401 aataaaactt ataaaatagc tgaaatggcc tgaaatcacc cggtttcagg ccatttttgt
     2461 ttgattttgg gtcaatttga gcaaaaaatg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn tgaaaaacat ggatttcaaa caaattcagg agttgatcaa
     2641 aatgatcaac aaatcgaata ttggcgaact gaccatagag caaaaagatt ttaaagttac
     2701 catcaaacaa aaagaggacc atgtaacaca ggttgttgct ccggctgctg tccatgctgc
     2761 tcctgttcac catgccccgg ccgcagcacc ttctgctccc gctgcccctg cagccgaaaa
//
LOCUS       DAORLS010000365         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19429_length_2819_cov_2.323082, whole genome shotgun sequence.
ACCESSION   DAORLS010000365 DAORLS010000000
VERSION     DAORLS010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19429_length_2819_cov_2.323082"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..>2819
                     /locus_tag="PK977_18340"
     CDS             237..>2819
                     /locus_tag="PK977_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20146.1"
                     /translation="MWLKKTYAILFLLSAIQLIAKAQQPNLVLPTGHTGIIRVACFSP
                     DGKYVVTGGEDKQVIVWEAHSGKKVHTLGTNISTVTFTSFSKDGRYLAAGTDSAIVIW
                     SFPDYRQLGIFELARRIIFSPDNKKVFVLGYDGNILQCALPSLKTEFEYRDNIKYESD
                     PQRKITRIAISPDGNSFCSISFDSSLIIRNTRNGKITATRKLKGDSWLCFFSADNMYI
                     NLVSDRGMERYHTGNESADSMLLRKWIVAAFLSNNGKYLLISWNMPGEVSALWDMEKY
                     KPIFQTDTIYSVNDTLAISDINESSYRTGTAAYGFPRFTGMIGAIETAPSGEFIRIGD
                     RIWNCKDNRLSYLIKDNDINRSDFSPDGRFLLVQQKSGSIGLWDLAAQKKLQVYETKV
                     STVQTAAISPDGNIFALAGTDRTVSINDLKTGQKLFLLRGHTASVKMVQFSSDSRKLL
                     TNSLDSTACLWDMQTGLLLKKFKGQHPELNPAWFDWKDSVMLPNVRIMNIAKIDDNGK
                     EYEVQMPEPLENPENILNTQSNNGKFAFFRIRGYPIWNVGGEDNKLQLESQWTYTSLV
                     HCFSQDSKWLAIAESSGSDSMLVGEIETGKWLFRKKLNTLSNDNWLYGSTEQLHFTKD
                     RKYLLAADAKSVIHIFSTANFMEVAQLPGKRAYLSADDKYLLALNKGNCDIYDFDNRK
                     LLYTYIMVDSTDYLVIDDRQRFDGTEQPRKMLYYSCGNEIVDLEQAKDLLWVPNLAER
                     IMKGDSINAKTLSELNICGLTPEVEDKSTAGQFRFHIQPRRGGLGETMVYVNGIEARR
                     YKPGQLKKQGDVYELILSKTELNNYFIAGKENPVTVKAYTADNAISSRGIIINQDKTQ
                     EKITA"
BASE COUNT          882 a          572 c          620 g          745 t
ORIGIN      
        1 tacctacagc ctgctggaag cgatgaacca atacccgacg ataaagatca gtgagctgaa
       61 aaagatagtg ggtgaaagag tggagcagct gacgaatggt atgcagaaac ctacttcgag
      121 gaatgagaat gttgcggtag actggagttt gtggtgaaat tgatcagacg tcttcatgca
      181 gataataccc gccggatggt atatgttgtt ctaaaggttt gtggtaactt tcaccaatgt
      241 ggctgaaaaa aacttatgca atcctttttt tactctctgc catacaactc attgcaaagg
      301 cacagcaacc taacctggtt ctgcctactg gacataccgg gataatcagg gtagcctgtt
      361 ttagtcctga tggcaaatat gtagtaacag gtggagagga caaacaagtt attgtttggg
      421 aggcccattc cggaaagaaa gtgcatactc ttgggaccaa tatcagtaca gttaccttta
      481 caagttttag taaagatggc cgctacctag cggctggtac tgattcagcg atcgtaatat
      541 ggtcatttcc cgactacaga caactgggaa tattcgaatt ggccaggcgt attatattct
      601 cacctgacaa caaaaaggtt tttgtattgg gttatgatgg aaacattttg caatgtgcac
      661 tcccatcact taaaaccgag tttgaataca gggataatat taaatatgaa tccgatccgc
      721 aacgaaaaat aactcgtata gctatcagcc ccgacgggaa ttcgttctgt tccatttcct
      781 ttgactcgtc tcttattatc aggaataccc gtaacgggaa gatcacagca accagaaaac
      841 tcaagggaga ttcctggctt tgcttttttt cagccgataa catgtatatc aacctggttt
      901 ccgacagagg tatggaacga taccacaccg gtaatgaatc agctgattct atgcttttgc
      961 gcaagtggat tgtggcagca ttcctaagca ataacgggaa atatcttctt atttcatgga
     1021 acatgcccgg agaggttagt gctctatggg atatggaaaa atacaaacca atatttcaaa
     1081 ctgatacgat ctactctgtc aacgacacac tggctatttc cgatattaat gaatccagtt
     1141 acaggactgg caccgctgct tatggttttc ccaggtttac aggaatgatt ggggcaatag
     1201 agactgcacc ttcaggagaa ttcattcgta ttggagaccg tatctggaat tgtaaagaca
     1261 atcggctatc gtatttaata aaagacaacg atatcaaccg atcggatttc agcccggatg
     1321 gcaggttcct tttggttcag caaaaaagcg gttctatagg tctatgggat ctggctgcac
     1381 aaaaaaaatt acaggtttat gaaactaaag ttagtactgt tcaaaccgca gcgataagcc
     1441 ctgacggaaa catctttgcc ttggccggga ccgatcgaac ggtgagcatc aatgatctta
     1501 aaaccggaca gaagcttttt ttgcttcggg ggcatactgc ttctgtaaaa atggttcagt
     1561 tcagcagcga ttcccgtaaa ctgctgacca actcactgga ttcaacggcc tgtctatggg
     1621 atatgcaaac aggcttgcta ttaaaaaaat ttaaaggtca gcatcccgag ctaaaccctg
     1681 catggttcga ctggaaagac tctgtaatgc taccaaacgt acggattatg aacatagcta
     1741 aaatagatga taatggcaaa gagtacgaag tacaaatgcc tgagcctttg gaaaatcctg
     1801 aaaatatcct aaatactcaa agcaataacg ggaaattcgc ctttttcaga attcgtggtt
     1861 atcccatatg gaacgtcggt ggtgaggata acaaactaca actggaaagt caatggactt
     1921 atacctctct tgttcattgt ttttcacagg atagtaaatg gctggcaatt gctgaaagca
     1981 gcgggagtga ttctatgtta gttggggaaa tagaaaccgg gaaatggctt ttccggaaga
     2041 aactaaacac actatccaat gacaactggc tctatggtag tactgagcaa cttcatttta
     2101 caaaagatcg taaatatcta cttgccgctg atgcaaaatc agttattcat attttttcaa
     2161 cagcaaactt catggaagtg gcacaattgc caggtaagcg ggcctatctg tctgcggatg
     2221 acaaatacct gcttgccttg aacaagggaa attgcgacat ttatgatttt gacaatagaa
     2281 aactcctata cacttatatc atggtcgatt caaccgatta ccttgtcatt gacgaccgcc
     2341 agcgtttcga cggtactgaa caaccaagga agatgttgta ttattcctgc ggcaatgaaa
     2401 ttgtagatct tgaacaggca aaggacttac tatgggttcc taatcttgcc gaacgcatca
     2461 tgaaaggcga cagcatcaac gccaaaactc tcagtgaact gaatatctgc ggtcttaccc
     2521 cggaagtgga agataaaagt acagcggggc aatttcggtt tcatatacaa ccccgccggg
     2581 gtggtttggg agaaacgatg gtatatgtaa atggtataga agcccgccgt tataaacctg
     2641 gtcaactaaa aaaacaaggt gatgtatatg agctgatact ctctaaaaca gaactcaata
     2701 attattttat tgccggcaaa gaaaacccgg tcacggtaaa agcctataca gccgacaatg
     2761 caatttcatc caggggcatc atcatcaatc aagataaaac acaggaaaag ataactgct
//
LOCUS       DAORLS010000366         2815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19468_length_2815_cov_1.991304, whole genome shotgun sequence.
ACCESSION   DAORLS010000366 DAORLS010000000
VERSION     DAORLS010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19468_length_2815_cov_1.991304"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PK977_18345"
     CDS             <1..450
                     /locus_tag="PK977_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF20147.1"
                     /translation="DSIPARKKLMLIDACHSGEVDKEEGIAIQKIADSLGLSKGIDLG
                     NSTTTTQHVGLKNSFELMQSLFVNVGKSTGATIISAAAGNQFALERGDLKNGVFTYSL
                     LEAMNTYPTIKISELKKIVGERVEQLTNGLQKPTSRNETIAVDWSLW"
     gene            523..>981
                     /locus_tag="PK977_18350"
     CDS             523..>981
                     /locus_tag="PK977_18350"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20148.1"
                     /translation="MKQKLFYGYFSLFSLLNLQTGAQQPRLVLPIGHSDWVSTVALSP
                     DGKKIITGSRDNTAKVWDVRTGKLLLNLIGHKEWVISAAFSPDGKKIITGSFDSTIKV
                     WELNSGKILIDIKEKNSKIKSAAFSSDGKKILTASLDNAARLWDAASGKLL"
     assembly_gap    982..1001
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1002..>2815
                     /locus_tag="PK977_18355"
     CDS             <1002..>2815
                     /locus_tag="PK977_18355"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF20149.1"
                     /translation="KGHTNNVWSASYSPEGSKILTYSEDGTAKIWSAISGQLLFNLNG
                     HSANISSASFSSDGKRLVTASSDKICKVWDMSTGQLLYELKGHTETVYAAVFTPDGNR
                     IISVSLNGVMKDWDANSGTYIKDLKGHTDAIWSINFSSDGSKIVTASNDKTAKVWDIA
                     SGQLLTDIKEQKAALYVAIFNRDGRQIITAAKDGSTKIWNAQTGSLVFDLKSHTKPVK
                     NIAFHPDGQTILTTTGSEAAKIWSVPTLSLLPVTIGRSDEKVLTFAFSPGGEQVATTS
                     RFEDVKIRDTRTGRLETTLEGTTSTSFAVFSPDGRQVVTTTFEGAGIVWNPVRARIIK
                     VLEDNNTFNMELNSATFSTDGKMIILTSPYKKATFWDTESGNLLGYFTSKRGFIQSIL
                     FSPDGQKVITTTGDDRAEVWDTKTFNVLKELSGHKQKVNAINFCYDGSKVITASDNTA
                     KIWDINTNKQLLDLKGHTASVNQAVFSRDGLTVVTVSQDNTCKVWNTKNGELNCTFFA
                     VDSTDYFVQIPSGYYKASAGATKLLHYVTQDLKVISFEQLDVKYNRPDKVLEAIGNAD
                     TALIKSYRKAWEKRIKKLGIDTTQFRDGYSVPEADFVN"
BASE COUNT          909 a          514 c          642 g          730 t
ORIGIN      
        1 gacagcatac cggcgagaaa gaaattaatg ctgatagatg cctgtcacag tggtgaagtg
       61 gataaggagg aaggtattgc tatacagaaa attgctgatt ccttagggct aagtaaggga
      121 atcgacttag gcaatagtac tactacaacc caacatgtgg gtttaaagaa cagttttgaa
      181 ttaatgcaat ccttgtttgt gaatgtgggc aagagcaccg gggctacgat catcagtgct
      241 gccgccggta accagtttgc cctggagcgg ggtgacctga agaatggtgt gtttacctac
      301 agcctgttag aagcaatgaa tacatacccc acaataaaaa tcagcgagct gaaaaaaata
      361 gtgggtgaaa gagtggagca actgacgaac ggcctacaga agccaacatc aaggaatgag
      421 actatagcgg tggactggag tttgtggtag aagttgaggt taagattaag attgagagaa
      481 taaggtgtta ttacctaaca gataaaaatg taatattagt aagtgaaaca aaaactattt
      541 tatggctatt tttccctttt ttcattattg aacctgcaga ccggggcaca gcagccgcgg
      601 ctggtgttgc cgataggaca tagtgactgg gtaagtacag ttgccttgag tccggatggg
      661 aaaaaaatta ttaccggttc gagagacaat actgcaaagg tttgggatgt aaggactggt
      721 aaattattac tcaatttaat tggacacaaa gagtgggtaa tttctgccgc atttagcccg
      781 gatgggaaaa aaattattac cggttcattt gattctacaa ttaaggtttg ggagttaaac
      841 tcaggcaaaa tccttattga cataaaggag aaaaacagta aaataaagtc tgcagctttt
      901 agttctgacg gaaagaaaat tcttacagct tcattagata atgctgccag gctatgggat
      961 gcggcttcgg gtaaactatt annnnnnnnn nnnnnnnnnn ntaaaagggc ataccaataa
     1021 tgtatggtct gcgtcttaca gtccggaggg cagtaaaatc ctgacttatt ccgaagatgg
     1081 tactgctaaa atctggagtg ctatttccgg ccagttgtta tttaatctaa atgggcattc
     1141 agctaatatt tcatctgcct cctttagttc tgacgggaag cgacttgtta cagcttcttc
     1201 agacaaaata tgcaaggtgt gggatatgag taccggccaa ttactgtatg aactaaaggg
     1261 acatacagaa accgtttatg ccgctgtttt cactcccgat gggaacagga ttatctctgt
     1321 atctctaaat ggcgtaatga aagattggga tgccaattcg ggtacctata taaaggatct
     1381 taaaggacat actgacgcta tatggtcaat taatttcagt tctgacggca gcaagatagt
     1441 aactgcctcc aatgacaaaa cggcaaaagt atgggacatc gcatcagggc agttattgac
     1501 agacattaaa gaacaaaagg ctgccttata tgttgctatt tttaatcggg atggccggca
     1561 aattataaca gcggccaaag acggatccac caaaatctgg aatgcacaaa caggaagctt
     1621 agtatttgac ttaaaaagcc ataccaaacc tgtaaaaaat attgcgtttc acccggacgg
     1681 acaaacaata ctcacaacaa ccggcagcga agccgccaaa atatggagtg taccaacact
     1741 tagcctttta cctgtcacga ttggacgatc agatgaaaag gtgttaacgt ttgcattcag
     1801 tccgggcgga gaacaggtcg ccactacttc tcgttttgag gatgttaaaa taagagatac
     1861 ccgaaccggg cgattagaaa caacactcga gggaacaacc agtaccagct ttgctgtatt
     1921 tagtcctgac ggcagacaag tagttaccac tacttttgaa ggtgccggaa ttgtctggaa
     1981 cccggttaga gccaggataa taaaagtact tgaagacaac aatacattta atatggaact
     2041 caacagtgct actttcagta ctgacggtaa aatgattata ctgacttccc cttataagaa
     2101 agctacgttt tgggatacag aatcaggaaa cctgctgggt tattttacca gtaagagagg
     2161 atttattcaa tcgatcctgt ttagtccaga cggacaaaaa gtgattacaa ctaccggcga
     2221 tgacagagca gaagtttggg atactaaaac gtttaatgtt cttaaagagc tctccgggca
     2281 taaacaaaaa gtaaatgcaa taaatttctg ttatgatgga agtaaagtta taacagcatc
     2341 tgacaataca gctaagatct gggatatcaa caccaataaa caattactgg atttaaaagg
     2401 gcataccgct tcggttaatc aggctgtttt ttcacgggat ggattaactg ttgtaactgt
     2461 atcacaggat aatacctgta aagtctggaa tacaaaaaat ggtgagttga actgtacttt
     2521 ttttgccgtt gacagcacag attattttgt tcagattcct tccggatatt ataaagcctc
     2581 tgcaggagct accaaactcc ttcactacgt tacccaagat ttaaaagtca tcagtttcga
     2641 gcagcttgat gtcaaataca accgccctga caaagtattg gaggccatag gtaatgcaga
     2701 taccgcattg ataaaaagct accgtaaggc atgggagaag cggattaaga aactgggcat
     2761 tgacactaca cagttccggg atgggtatag tgtacccgag gctgattttg taaac
//
LOCUS       DAORLS010000367         2802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19587_length_2802_cov_1.899527, whole genome shotgun sequence.
ACCESSION   DAORLS010000367 DAORLS010000000
VERSION     DAORLS010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19587_length_2802_cov_1.899527"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PK977_18360"
     CDS             complement(<1..485)
                     /locus_tag="PK977_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20150.1"
                     /translation="MKQKLTIALLGIAAVTLFITGCRKRDAIEADNFVAFETAAQGMT
                     AAENSITVRLELSRNTDRDIAVTINLNEMGVAYATDYTTTPAATAGKLTVTIPSGNNE
                     ASFTVTKVNGVLFDGDEKINFDLYSSAAPVFIGATKQFTLSFGEIVANNPSLTINGGG
                     V"
     gene            complement(523..>2082)
                     /locus_tag="PK977_18365"
     CDS             complement(523..>2082)
                     /locus_tag="PK977_18365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20151.1"
                     /translation="NYNLSVTARLHEETVGKEYGIKQGIHNQQAGLTWKRNNWEAGGS
                     FAHNYFGGWQDTAVGRELVWHKKDQLIANGFIGYTKGKLSLRYRIDGLDEVITNPGNY
                     SPFPDQISGDTLAYDQEYLSQRVMHQLQGSYFVSSKLNFQVQTSYTDYSRQVFSTTVN
                     KKNGDVRLDPGEGKQSVVDFRGFTFRGTALYRLSDKVSFQPGVDINLDRGEGERLKSG
                     SNAVDDYAFFITSEITPNSKISIRPGLRFIKNSVYDAPPFIPSINAKLALTNDIDFRI
                     SYARGFRSPSLRELYFSFFDANHQVVGNPDLKAETSHSFTGSVNMKKKKDNGALYHLQ
                     LGGFYNDVKNLIDYVFTQSSDTAKLYNVLNSKTSGVNLTAGYGYKNWNFNAGFAYTGF
                     YNDQYSADKSLPELVWSAEANASASYHFKKMGLDLNLFYKLVGKRPRYVTSGPDIILS
                     EQDAYHMADFTVSKKIIRMLSLNAGVRNLFDVSRINSSFVQGGIHTGGGLNVGTGRSY
                     FATLAFNWNKK"
     assembly_gap    2083..2194
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2195..>2548)
                     /locus_tag="PK977_18370"
     CDS             complement(<2195..>2548)
                     /locus_tag="PK977_18370"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HRF20152.1"
                     /translation="LSLNAQVLRIPPPEDTTKAKDLEEVVITGQYKPQSVKNSVYQVK
                     VIGKERILKQGATRLQDVLRNELNMRFSQDLATGGSAINMLGLSGQNVKILIDGLPVI
                     GRQGVNNEFDISQID"
     assembly_gap    2549..2558
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          712 a          554 c          623 g          791 t
ORIGIN      
        1 aatacaccgc caccgttgat agtgagtgag ggattattgg ccactatctc gccaaagctg
       61 agggtgaact gtttggtagc gccgatgaat accggtgctg cagaactgta cagatcaaaa
      121 ttgatctttt catctccatc aaaaagcaca ccgtttactt tggttacagt aaaggatgct
      181 tcattattac cggaaggaat ggtaacagtg agtttgccgg ctgtggcagc aggggtggta
      241 gtataatctg ttgcataggc aacacccatt tcattcagat tgatagtaac agcaatgtcc
      301 ctgtcggtat tgcgggagag ttcgaggcga acagtgatgg aattttcagc ggcggtcata
      361 ccctgtgctg cagtttcaaa cgccacaaag ttgtctgctt ctattgcatc tcttttgcgg
      421 cagcctgtga tgaacagtgt aactgctgca atgcccagaa gtgctattgt tagtttttgt
      481 ttcatcctgt agtaattagt atatgataaa atttttgttg acttattttt tgttccagtt
      541 aaatgccagt gtggcgaaat aagaacggcc ggttcctaca ttcagtccgc cgccggtatg
      601 tataccgccc tgtacgaagc tgctgttgat acggcttaca tcaaagaggt tgcgtacacc
      661 ggcattgagg ctgagcatcc ggatgatctt tttactgaca gtaaagtcgg ccatgtgata
      721 agcatcctgt tcggataaaa taatatcggg gccgctggta acatagcggg gacgtttgcc
      781 taccagtttg taaaacaggt tcaggtcgag gcccatcttt ttaaaatgat aactggcact
      841 ggcattggct tcggcagacc ataccagttc aggaagactt ttgtctgcac tatactggtc
      901 attatagaaa ccagtgtaag caaagccggc attgaagttc cagttcttgt aaccgtaacc
      961 tgctgtcagg tttacaccgg atgttttgct gttaagtaca ttatacagtt tggcggtgtc
     1021 agagctttgg gtaaatacat aatcgatcag gttctttaca tcattgtaaa aaccgcccag
     1081 ttgcaaatga tacaaagcgc cgttatcttt tttcttcttc atgttcacag aaccggtaaa
     1141 gctgtgagag gtttctgctt tcaggtcagg attgcccacc acctggtgat tggcatcgaa
     1201 gaagctgaag tagagttccc gaagggaagg agaacggaaa ccccgtgcat aagaaatacg
     1261 aaaatcaata tcgttagtca aagccagttt ggcattgatg gagggaatga acggaggtgc
     1321 atcatacacg gagtttttaa taaagcgcaa acccggacga atactgatct tgctattggg
     1381 agtgatttct gaagtgatga agaaagcata atcatctaca gcattggagc cgctctttaa
     1441 tctttctcct tcgccacgat caaggttaat atctacaccg ggctgaaaac ttactttatc
     1501 agataacctg tacaaggcag taccacgaaa tgtgaatccc ctgaaatcaa caaccgactg
     1561 tttgccttct ccgggatcta atcttacatc gccgttcttt ttattgacag tagtagagaa
     1621 aacctggcgg ctgtaatcgg tgtaagaagt ttgtacctgg aaattcagct tgctgctgac
     1681 aaaataagag ccctgcaact ggtgcattac tctttgtgaa agatattcct ggtcgtaagc
     1741 cagtgtatcg ccggagatct ggtcaggaaa aggagagtag ttgcccgggt tggtgatcac
     1801 ttcatctaat ccatcaatac ggtagcgaag gctgagttta ccttttgtat aaccgataaa
     1861 accgttggcg atcagctgat cttttttatg ccagaccagt tcacggccta cagcggtatc
     1921 ctgccaccca ccgaaatagt tgtgggcaaa tgatccgccg gcttcccagt tgttgcgctt
     1981 ccaggtaagt ccggcctgct ggttatggat accttgtttg ataccatatt ctttacccac
     2041 tgtttcttca tgtagcctgg ctgtaacgga aagattgtag ttnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncatcga tttggctgat gtcaaattca
     2221 ttatttacac cctggcggcc aataacaggc agaccatcga tcaggatctt tacgttttga
     2281 ccgcttaagc ccagcatatt gatggcagaa ccaccggtgg caaggtcttg tgagaaacgc
     2341 atattgagtt cgtttcttaa tacatcctgc aaacgggtag cgccttgttt taggattctt
     2401 tctttgccga ttactttaac ctggtataca gagtttttaa ctgattgggg tttgtattgt
     2461 cccgtgatga cgacttcttc gaggtcttta gcttttgtag tgtcttcggg aggcggaatg
     2521 cgcagcacct gtgcattgag tgaaagtgnn nnnnnnnnac agcttattgc agttgtcact
     2581 agtattttgt ttctcataat aattaaaaag tttcgggcac aaagttttgt cagcattcat
     2641 tgagaattat cacatcattg tcaggaaaca gtgtcctgaa tttctttgac aaaaaaatga
     2701 cagcacatag ttagttaatc agtgcacatg ctgacaaacg tcatgtgata tttttttttc
     2761 tgctatgaga aaaaacaaaa aagcctctct tgtttaaaga aa
//
LOCUS       DAORLS010000368         2796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19637_length_2796_cov_3.005472, whole genome shotgun sequence.
ACCESSION   DAORLS010000368 DAORLS010000000
VERSION     DAORLS010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19637_length_2796_cov_3.005472"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PK977_18375"
     CDS             complement(<1..1120)
                     /locus_tag="PK977_18375"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF20153.1"
                     /translation="MKSLLISVAFFFSLTTSAQNTLDLLQRTMELFDKGDFKNTIPVA
                     EKALVAVKKDFGDKSPFVSGLVMFLAISHLRLYELDKAATYFLQQKDLLYSNGEKDLS
                     YLSCLNSLGFLYREKGEYKKSESYYQQILTITKKNYGINDSSYAKGLNNLASLYQHLG
                     QYSKAEQLFLQAAQIMKLVMGERSAGYLSSQNNIATLYQQMGLYAKASAVFIAVANAR
                     KAVLGELHADYAQSLNNLANNYASLGNFKEAERAFVLAAEIYKKTTGTLHPEYAATIN
                     NLGELYVQTGEYDKALIYYNSSLDIRKKLYGTEHPDYALSLNNFGTLYQQTGQYESAE
                     NYFLTANSIIEKNLGKNHPDYVTQLNNLAGLYQSMGLYT"
     gene            complement(1189..>2796)
                     /locus_tag="PK977_18380"
     CDS             complement(1189..>2796)
                     /locus_tag="PK977_18380"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF20154.1"
                     /translation="LEGDLMVADYNNSFLYNYPRASATFVKNNFDLQLVYKSMSLADT
                     RNMLNAVMQSKDTTIRRIFSEWQANKNILTKQSALPADKRRPDLTTIQALTEQQEKEL
                     NRRSSDFRNQQEALRITSAEVQKSLDDDEAAIEFVKFWLYTNKRWTDTVLYAAYILRK
                     SDSIPRFVVLCNEKQLQQVFDNAGKNTTAMVNSFYRGLDIEKQQPAQLGAELFKLIWA
                     PLEPHLKGIKKISYSPAGKLFTIAFHALPADSTAVLMDRYQLHQYTSTRQIVWRSQQK
                     GGVNPSGITLFGDPDYTWQAGAVTQNKPAVSTKGVLPPRRGGEGSWSPLPGTAAEVKK
                     INDLFAQNKIATRLFLQQKASEENLKSQDGRSSQILHIATHGYFLPQSLKRKQKEESK
                     NTYVLSEDPLIRSGLILAGGNNAWTGKTIPEGAEDGIATAYEIAQLNLSNTELVVLSA
                     CETALGDVRGSEGVFGLQRAFKMAGVKKMIVSLWQVPDKETAELMTTFYSWWLKGKTT
                     DDAFAMAQADMRKKYSPFYWAAFVLVE"
BASE COUNT          718 a          617 c          558 g          903 t
ORIGIN      
        1 tcgtatataa gcccattgac tggtacagac ctgccaggtt attcagttgc gtcacataat
       61 ccggatggtt tttacccaga tttttttcga taatactatt ggctgttaaa aagtagtttt
      121 ctgctgattc atattggcct gtttgctggt agagagtccc gaaattatta aggcttaagg
      181 cataatccgg gtgctcggta ccataaagct tctttcggat gtctaaagaa gaattataat
      241 agataagggc tttatcatat tcccctgttt gtacatatag ctctcccaaa ttattgatag
      301 tagccgcata ctccggatga agggtacctg tggttttctt atatatttct gcagcaagta
      361 caaatgccct ttctgcttct ttaaaattac ccaatgaagc gtaattattt gcaagattat
      421 tcaggctttg cgcataatct gcatgaagct cacccaacac agcttttctt gcatttgcca
      481 cagcaataaa aacagcactt gcttttgcat acaaacccat ctgctggtaa agtgtggcaa
      541 tattattttg cgaggaaaga tacccagcag atctttcacc cataaccagc ttcattattt
      601 gtgctgcctg aaggaaaagt tgttcagcct tgctgtattg cccgaggtgc tggtacaatg
      661 acgcaagatt attcaatccc ttggcatagc tgctatcatt gatgccatag tttttttttg
      721 tgatagttaa aatctgctgg taataagatt ctgacttttt atactctccc ttttcccggt
      781 aaagaaaccc aagactgtta aggcatgaca gatatgaaag atccttttct ccgttggaat
      841 acaacaaatc cttttgttgt aaaaaatagg ttgctgcttt gtccagttca tataaccgca
      901 gatgacttat tgccaggaac attaccaggc cgcttacaaa agggctcttg tcaccaaaat
      961 cttttttaac agcaaccagg gccttttcag caacagggat tgtgtttttg aaatcgcctt
     1021 tgtcaaaaag ttccattgtc ctttgcagca ggtccagggt gttttgagct gaggtagtca
     1081 gtgaaaaaaa gaaagcaact gaaatcagta atgatttcat ggcagcagtt ttggttgaat
     1141 agactgttaa tttactaaat cccttttgaa ctttcccgga aaaaagactt actctaccag
     1201 tacaaatgca gcccaataga aaggagaata ttttttacgc atatctgcct gtgccatagc
     1261 aaatgcatcg tctgttgttt ttcctttcag ccaccaggaa taaaaagttg tcatcagttc
     1321 agcagtttct ttatccggca cctgccacag gctgacgatc atttttttta cacccgccat
     1381 tttaaatgcc cgttgcagac caaatactcc ttcacttccc cttacatcac ccaaagccgt
     1441 ttcacaggca ctcagcacaa cgagttccgt attactcaga tttagctggg ctatttcata
     1501 ggcagttgca ataccatctt cagcaccttc cggaatagtt tttccggtcc acgcattatt
     1561 gcctccggct aaaatcaatc cgctcctgat caacggatct tctgaaagca cataggtgtt
     1621 ttttgattct tccttctgtt ttctttttag gctttggggc agaaaatatc catgtgtggc
     1681 aatatgcagt atttgtgagg agcggccatc ctgcgactta agattttctt cgcttgcttt
     1741 ctgttgcagg aataaccgtg tggctatttt gttttgagca aaaagatcat tgatcttttt
     1801 tacttctgca gctgttcccg gcaaagggga ccagcttccc tcaccaccac gcctgggagg
     1861 taatactcct ttagttgaaa cagcaggttt attttgtgta acagctccgg cctgccatgt
     1921 ataatcagga tcaccgaata atgtaatgcc cgaaggatta actccgccct tttgctggct
     1981 tcgccatact atctgccttg tgctggtgta ctggtgaagc tggtacctgt ccatcaatac
     2041 ggccgtgctg tctgcaggca acgcatgaaa agcaatagtg aataattttc cagcaggtga
     2101 atagctgatc ttttttattc ctttcaggtg aggctccagg ggagcccaaa tcagtttaaa
     2161 caattctgcc cctaactgtg ccggttgctg cttttctata tcaagccctc ggtaaaaact
     2221 gtttaccatg gcagtggtat tcttacctgc attgtcaaaa acctgttgca actgcttttc
     2281 attacaaagg acaacaaacc gtggtatgga atctgattta cgaaggatat atgcagcata
     2341 taaaaccgtg tccgtccatc ttttattagt gtacaaccag aattttacga attcaatggc
     2401 ggcctcatca tcgtccagtg atttttgtac ttctgctgat gtgatgcgca atgcttcctg
     2461 ctggtttctg aaatcggatg agcggcggtt cagttctttc tcctgttgct cggtcagtgc
     2521 ttgaatagtt gtcagatctg gccttctttt atctgcaggt aatgccgatt gctttgtcag
     2581 aatattttta ttggcctgcc attctgaaaa tattctccgg atagtggtgt ctttgctttg
     2641 cattactgca ttcagcatat tccgggtatc agccaggctc attgatttgt ataccagctg
     2701 cagatcaaaa ttgtttttta cgaatgtggc tgaagcccgg ggatagttgt acaaaaaact
     2761 attattgtaa tctgctacca tcaggtctcc ttccag
//
LOCUS       DAORLS010000369         2781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19790_length_2781_cov_3.526045, whole genome shotgun sequence.
ACCESSION   DAORLS010000369 DAORLS010000000
VERSION     DAORLS010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19790_length_2781_cov_3.526045"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..704
                     /locus_tag="PK977_18385"
     CDS             237..704
                     /locus_tag="PK977_18385"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRF20155.1"
                     /translation="MIKKGKIARELYFISKGLLRLYYTKDTEDITAFIFREHLFASCY
                     ESFLRQTPGNQSLDTLEDAELLVISYDDLQTLYKKIPKMETVARKISEQRFINAQMIL
                     SSYILDSPEERWKKFEATQGDLLLRVPHHMIASFLGITPVSLSRIRKRLMEGS"
     gene            772..>951
                     /locus_tag="PK977_18390"
     CDS             772..>951
                     /locus_tag="PK977_18390"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF20156.1"
                     /translation="MNKQIITEIIINSSKEKVWQVLTDFSSYHVWNPFIISIEGELKK
                     GSKLKNTLRNGDKTIR"
     assembly_gap    952..968
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <969..1209
                     /locus_tag="PK977_18395"
     CDS             <969..1209
                     /locus_tag="PK977_18395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20157.1"
                     /translation="QEVKYGESFSWLGNLFFKGLFDGVHSFIIEDAGNNHVKLIHSEI
                     FSGVLSSFIFKKIGEETRNNFIEMNLALKNKAESI"
     gene            1236..2303
                     /locus_tag="PK977_18400"
     CDS             1236..2303
                     /locus_tag="PK977_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRF20158.1"
                     /translation="MNGNRIVYIDNIKVFLTGLVVAHHAAQAYGTTGGVWVVNDPFKA
                     DWLRDFFFLNASYMMGLYFFISGYFMVFSIKMKSNLAFMNDRFKRLGIPLLFVTLFIF
                     LPFNYFFTESNRNVFSFFADSYLHKPPVATGHLWFVASLLAYSIVYNILFHKKFIDLA
                     KKAGSLTVSHILIFTVVITVLCAAVRLKYPIDTWRTWLIPLEPAHLSQYFLLFLAGAF
                     FNHTSWLNQLKSKYSLAFFGFAVFIYIVQRYLPDEIKNYWITESLIESVMCIGISIGL
                     LALFKKYGNSTNAFVSKLSANAYGIYLVHLFIVILLQYSIINLLLNANIKFIAVTIAG
                     ILLSLLLSMLIRRSRYIRQII"
     gene            2321..2710
                     /locus_tag="PK977_18405"
     CDS             2321..2710
                     /locus_tag="PK977_18405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF20159.1"
                     /translation="MKAVQFRIARPTNQLAEITRFYTDGLGLKVIGQFAGHEGYDGVM
                     IGLPDSEHHLEFTKHVNETALPEPTNEHLLVFYFNNSTEYTEANKRLEQMNCFPVKPE
                     NPYWEGKSETYEDPDKWRIVLFNGIFL"
BASE COUNT          876 a          509 c          522 g          857 t
ORIGIN      
        1 ttcgggcgac cgggcttcga atacccggcc tgcggaaata cgtggccgtt ggctgaaagt
       61 ttaaaccctc tgattaatca accggtatta tttgcttgct atgtttaccc cattatttga
      121 ctacatatca caattcaaga atattactaa tgaggaaaag catatcatag aaagctattt
      181 cacattcagg caagtgccaa aaaccgggca tcatcagcgg agcaggactt acttctttga
      241 taaaaaaagg aaaaattgca agagagttat attttataag caaggggcta ctgcggctat
      301 actacaccaa agacacagaa gacattaccg cttttatttt cagagagcac ctttttgcaa
      361 gttgctatga aagttttctg cgccagacgc ctggcaatca atcactagat accctggaag
      421 atgccgaact tcttgttatt agttatgatg atttgcaaac actttataaa aagattccca
      481 aaatggaaac ggttgcccgc aagatatccg aacagcggtt tatcaatgca caaatgatat
      541 tgtcgtccta tatacttgat agccctgaag aaagatggaa gaaatttgaa gctacacaag
      601 gtgatttact acttcgtgta ccgcatcata tgattgcctc cttcctgggt attacacctg
      661 tttccctcag tcgaatccga aaacggctta tggaaggaag ctgatttctt tacaaaagat
      721 aataaaccta ctattctacc ttcatagctt tgttccataa ataaatacgt tatgaacaag
      781 cagattataa cagaaataat aattaattct tcgaaggaga aagtgtggca ggtactcaca
      841 gatttctctt cttatcatgt atggaatccc tttattataa gtatagaagg ggagttaaaa
      901 aaaggaagca agctaaaaaa cacattacga aacggagata agactattag gnnnnnnnnn
      961 nnnnnnnncc aagaagtgaa atacggcgaa agtttctcct ggctcggcaa tcttttcttt
     1021 aagggattgt ttgacggtgt ccattcattt ataatagaag atgcgggtaa caatcatgtt
     1081 aagttgatac attcagaaat attcagtgga gtgctcagtt catttatttt taaaaaaata
     1141 ggggaagaaa caagaaataa ttttattgaa atgaacctgg cactgaaaaa caaagcagag
     1201 tcaatctaat tggtaaaagt ctcgaatcaa ataggatgaa cggcaaccgc attgtttata
     1261 tagataatat taaagtgttt cttaccggcc tggtagtggc gcaccatgct gctcaagcct
     1321 atggtactac cgggggtgta tgggttgtaa acgatccttt caaagctgac tggttgaggg
     1381 atttcttttt tttaaatgca tcttacatga tgggtcttta tttttttatc tctggttact
     1441 ttatggtttt cagtattaaa atgaaatcaa accttgcatt catgaatgac cggtttaaaa
     1501 gattaggtat acctttacta ttcgttacgt tattcatctt tctcccattc aattattttt
     1561 ttacagaaag caaccgtaat gtatttagtt tctttgccga cagttacctg cacaagccgc
     1621 cggttgctac aggccatctt tggtttgtag cctcattact ggcctattca atagtctaca
     1681 atattttatt tcataaaaaa tttatcgatc ttgctaaaaa agccggctca ttaactgtta
     1741 gccatatttt aatatttaca gtggtcataa cagtcttatg tgctgcagta cgattgaaat
     1801 atccgataga tacctggcga acctggctca taccgctgga gcctgctcac ctgtcgcaat
     1861 actttttgct ttttttagca ggggcttttt tcaatcatac tagttggcta aatcagttaa
     1921 aaagtaaata tagcttagct ttttttggct ttgcagtttt catctatata gttcaaaggt
     1981 atctgccgga tgaaataaaa aattattgga taaccgaatc gcttatagaa tcagtaatgt
     2041 gtattggaat tagcataggt ctccttgcac ttttcaaaaa gtatggaaac agtacgaatg
     2101 catttgtctc aaaattatca gcgaatgcct atggcatata cctggtgcat ttatttatcg
     2161 tcattttatt gcagtatagc ataataaatc ttttgctcaa tgccaatatt aagttcatag
     2221 cagttaccat tgccggtatt ttgctttctc tattattatc tatgctaatc agacgcagca
     2281 gatatatcag gcaaattata taatcacata attcagaaaa atgaaagcag tacaattcag
     2341 aatagctaga cccaccaatc aactggcaga aatcacaagg ttttatacag atgggcttgg
     2401 tttaaaggtt atcggacaat ttgcaggtca cgagggatat gacggtgtta tgatagggct
     2461 tcccgacagt gaacatcatc ttgaatttac taaacatgta aatgagacag cattaccgga
     2521 gcctaccaat gagcatctgc tagtttttta tttcaacaat tctacggaat atacagaagc
     2581 taataagagg cttgagcaaa tgaattgctt tcctgtaaaa cccgaaaatc catactggga
     2641 aggtaaaagt gaaacgtatg aagatcccga caagtggagg atcgtactct tcaacggcat
     2701 ttttttataa ctcgaaaaac cttcagccaa catcgtattt cctcaagccg ggcgcgacct
     2761 ggaaatttgg gcatcatttt t
//
LOCUS       DAORLS010000370         2770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19885_length_2770_cov_2.538858, whole genome shotgun sequence.
ACCESSION   DAORLS010000370 DAORLS010000000
VERSION     DAORLS010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19885_length_2770_cov_2.538858"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK977_18410"
     CDS             complement(<1..586)
                     /locus_tag="PK977_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRF20160.1"
                     /translation="MKLPEHPCNCEGRRSFLKKTTAITVLSIAPKALLKAGEQNEAIA
                     SFFEKAPIKLQINGKDYSIAVDPSMSLLDMLREQLGLTGTKKGCDQGHCGACIVHVDG
                     QRVNSCLSVAVENNGKKITTIEGIGQGDQLHPMQEAFIKHDAFQCGYCTPGQIMSAVA
                     CIKEGKANTPDEIREYMSGNICRCGAYPNIVDAIM"
     gene            750..>1143
                     /locus_tag="PK977_18415"
     CDS             750..>1143
                     /locus_tag="PK977_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF20161.1"
                     /translation="MDRIYLDNAATTALDKEVLDAMLPYMTSHFGNPSSIYSYGRESR
                     LAIETARKTVARILNAHPGEIFFTSGGTESDNMAISSAINDLGCRHVITSPIEHHAVL
                     HAVEHFDHEDVVTSSFVKIFPDGHVDLND"
     assembly_gap    1144..1189
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1190..1894
                     /locus_tag="PK977_18420"
     CDS             <1190..1894
                     /locus_tag="PK977_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF20162.1"
                     /translation="RCLVSLMHANNEIGNLLDIYAAGEICKKYNAIFHSDTVQTVGHY
                     KLDLRSKPVHFATGAGHKFHGPKGIGILYINDNIKVNPIIFGGGQERNMRAGTENLYG
                     IVGFAKALEMADANMEKDSAYIQSLKTYMIEQLQKHIKGVQFNGDYAGKSLYTVLNAS
                     FPKTEKSEMILFNLDMQGICVSGGSACSSGADVGSHVIRAISNNPNLVPVRFSFCKHN
                     TKEEIDTVVSKLKDLI"
     gene            complement(1906..2127)
                     /locus_tag="PK977_18425"
     CDS             complement(1906..2127)
                     /locus_tag="PK977_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HRF20163.1"
                     /translation="MNKLKSFVEWNAFGVCSAIGNRLGIATSKIRQYFMYASILTLGS
                     PIIIYMILAFWRNIRKYIWAAKRNPWYYL"
     gene            2313..>2770
                     /locus_tag="PK977_18430"
     CDS             2313..>2770
                     /locus_tag="PK977_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRF20164.1"
                     /translation="MVFDLDLIKKTYTEMPAKVDAARKALGRPLTLAEKILYAHLWKG
                     VAIKEFKRGKDYVDFAPDRVAMQDATAQMALLQFDTTGRKKVAVPSTVHCDHLIVAKN
                     ESKTDLAKAVTDNEEVYNFLSSISNKYGIGFWKPGAGIIHQVVLENYAFPG"
BASE COUNT          775 a          582 c          597 g          770 t
ORIGIN      
        1 ccatgatagc gtcaacaata ttgggataag ccccgcaacg gcagatattg ccactcatgt
       61 attcccgtat ctcgtcaggc gtatttgctt ttccttcctt gatgcaggca acagccgaca
      121 taatttgtcc gggtgtgcaa taaccgcatt gaaaagcatc gtgtttgata aatgcttcct
      181 gcatcgggtg aagctgatcg ccttgtccta tcccttcgat cgtggtgatt tttttcccat
      241 tgttttccac tgccaccgac aaacaggagt ttactctttg tccgtctaca tgtacaatgc
      301 aagccccgca atgtccctgg tcgcatccct tttttgttcc tgttagtccc agttgctctc
      361 tcagcatgtc aagcaaactc atggaggggt ctacagcgat ggagtagtct ttgccattga
      421 tctgtaattt aatgggcgcc ttctcaaaga aagaagcaat ggcttcattt tgctcgcctg
      481 ctttaagcag ggccttgggt gcaatgctta aaacagtgat ggcagtagtt tttttcagaa
      541 agctccggcg gccttcgcag ttgcagggat gttcaggaag tttcatgata taagaatgta
      601 ttacaaatta gtacgtttct ttcagggcag aaaaaaatgt tagttcagaa tcagatttta
      661 gtccatcacc agatcctgtt tgcgcacagg atattatgca cccggtgtaa tgcctatatt
      721 tgctgccaat ttattgactt gccccgctta tggatcgtat ttacttagat aatgccgcta
      781 ctactgcgct tgacaaagaa gtgctggatg ccatgctgcc atatatgact tcgcattttg
      841 gtaacccttc ctctatttat tcctatggca gggaaagcag gctggctata gaaacagccc
      901 gtaaaacagt ggccaggatc ctgaatgccc atcccggcga aattttcttt acaagcgggg
      961 gaacggaaag tgataacatg gccatttctt ctgcgatcaa cgatctcggt tgccgtcatg
     1021 tcatcacctc accaatagaa caccatgctg tattacacgc cgtggagcat tttgaccatg
     1081 aagatgtggt gaccagcagt tttgtaaaaa tatttcctga cggacatgtt gatttgaatg
     1141 accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gatgtttggt
     1201 cagcctgatg catgccaata atgagatagg aaacctgctt gatatatatg cagcaggaga
     1261 aatatgtaag aaatacaatg ccatctttca ttccgatacg gtgcaaacag ttggacatta
     1321 taagctggac ctcagaagta agcccgtaca ttttgctacc ggcgcaggcc ataagtttca
     1381 tggaccaaag ggcataggta ttctttacat caatgataat attaaagtaa accctataat
     1441 ctttggaggt ggccaggaaa gaaatatgag ggccgggact gaaaatctct atggaattgt
     1501 aggctttgca aaagccctgg aaatggccga tgccaatatg gaaaaagaca gtgcttacat
     1561 tcagtcgctc aaaacctata tgatcgaaca actgcaaaag catatcaaag gagtgcagtt
     1621 caatggagat tatgcaggaa aaagtttata cactgtctta aatgcctctt tcccgaaaac
     1681 ggaaaaatca gaaatgattc tttttaacct ggatatgcag ggtatttgtg tttcgggcgg
     1741 cagtgcctgc agcagcggag cagatgtggg cagccatgta atcagggcta tcagcaataa
     1801 cccgaacctg gtacctgtac gtttctcctt ctgcaagcat aatacaaaag aagaaataga
     1861 tacagttgta tctaagttga aagacctgat ttaaaccgga tcttatcaga ggtaatacca
     1921 gggatttctt ttagccgccc agatgtattt gcggatattt ctccaaaagg caagtatcat
     1981 gtagataatg ataggtgacc ccagtgtcag gatagaggca tacataaaat actgcctgat
     2041 cttgctggta gcaataccca gcctgttgcc aatggccgaa caaacgccaa aggcattcca
     2101 ttctacgaag ctttttaatt tgttcatatt gctaatttag aaattttgaa caaaccatcc
     2161 aattcggggt tattcagaca ggctatttcc gtaattttgc ggcactcaaa tatttcatat
     2221 tgaagattta atatttgctc tttcaggcct attgtcaggg tgtttaccgc ttaatattca
     2281 atcttcaatc gaacatcata aaacagctta tcatggtatt tgacctcgac ctcattaaaa
     2341 agacttatac tgaaatgcca gccaaagtgg atgctgcccg caaggctttg ggacgtccgt
     2401 taacattggc agagaagatt ttgtatgctc acttgtggaa aggcgtagca ataaaggaat
     2461 ttaaaagagg taaagattat gtcgattttg ccccggacag ggtagccatg caggacgcca
     2521 ctgcacaaat ggccctgctg cagtttgata ccaccggccg taagaaagtg gctgtacctt
     2581 caacagtaca ttgcgaccac ctgatcgtgg ctaaaaacga aagtaaaact gaccttgcta
     2641 aggccgttac cgataatgaa gaggtttata atttcctttc ttcgatttct aataaatacg
     2701 gaataggttt ctggaaaccc ggtgccggta tcattcacca ggtagtactc gagaattatg
     2761 catttccagg
//
LOCUS       DAORLS010000371         2764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19944_length_2764_cov_1.790329, whole genome shotgun sequence.
ACCESSION   DAORLS010000371 DAORLS010000000
VERSION     DAORLS010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19944_length_2764_cov_1.790329"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>227)
                     /locus_tag="PK977_18435"
     CDS             complement(<1..>227)
                     /locus_tag="PK977_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20165.1"
                     /translation="LTGAAGLNINFPDEEPKHDIKLIEKDEPVEKKIELVEKQPTIEN
                     EIVSDLLHQHDTMDMLEEEEEEQVLEMIPPP"
     assembly_gap    228..331
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<332..>627)
                     /locus_tag="PK977_18440"
     CDS             complement(<332..>627)
                     /locus_tag="PK977_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRF20166.1"
                     /translation="GINLLFRRKVFSIWRNLKYVTVGLLVLSVVLAFLFRSNEFPFGG
                     AVGNMINDKMTGALGIFGTGAVLFLLAFGYFIWQFNPSFNLPQKKQAEETATAE"
     assembly_gap    628..703
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<704..1000)
                     /locus_tag="PK977_18445"
     CDS             complement(<704..1000)
                     /locus_tag="PK977_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRF20167.1"
                     /translation="MAEKQKKKKSPAKKPEGSDVKPFRAEKEESIDLKALARDERTWK
                     IVGSVFLLIAVFLFISFISYLFTWKEDQAVAQQGFSALFDTDEPVANLLGRLGAV"
     gene            1165..>2764
                     /locus_tag="PK977_18450"
     CDS             1165..>2764
                     /locus_tag="PK977_18450"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HRF20168.1"
                     /translation="MTRLYRSFIFLTMSLMLLGSKAYTQDSLQQQFTDISKISLVRQL
                     SKTINSTYTDKNIELAKEYAQLKFSPGIKHKGTVPNEYVTRKLALQFNISNSADSTVS
                     VYFFPGFYYSRIDLYQKTATGLVNIPSVLPSNEDSIGYRLISLGGHDSATIIAHLSFV
                     KTYINTIRPRLIHKDHIDSFLAQVRSSHNNDNLVTYVFCGLLLMMILFSFSNFLQGAN
                     REFFYYSLYAFLLGSMLFTKATYDFQTTHITFFLEAYLDFILQGLGIIFYMIFMQKFL
                     NTRQNHPFLFKFYNAGIIMLAVTLLLYSYLHFFTDNFSLENTVENASKLLLLLMVLIF
                     LVYSIRNWKDRLMRYLFWGNLCLFLFSVISQLSVMMEQLFKDLPGVFSSALFYYEIGL
                     FLELVFFLAGLNHKNRRSIIQRTKETETLKAQNLMQQYEKEIAVFKAQQAERERISAD
                     MHDELGSGMTAIRLMSEIARNKMKENTPVEIHKISQSADEVLNKMNAIIWSMNSGNDT
                     LDNLLSYIRSYSLEYLDNTNIDCKV"
BASE COUNT          735 a          587 c          502 g          760 t
ORIGIN      
        1 aggcggaggt atcatttcca gtacctgctc ttcctcctcc tcttccagca tatccatcgt
       61 atcatgctgg tgcagcaggt cgcttacaat ctcattctct atcgttggtt gtttttctac
      121 cagttcaatt ttcttttcaa ccggctcatc cttttctatc agtttgatat catgcttcgg
      181 ctcttcatcg ggaaagttga tattcaaacc cgcagcgccg gttaatgnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcttccgct gttgctgttt cttcagcctg
      361 ttttttctgc ggcaggttaa aagatggatt gaactgccag atgaaataac caaaggctag
      421 caggaacaga acggcacctg ttccaaagat gcccaatgcc ccggtcattt tatcattgat
      481 catattaccc acagcgccgc caaaaggaaa ttcgttgctt ctaaaaagaa aagccagcac
      541 aacggataac accagcaggc caaccgttac atatttaagg tttcgccaga tagaaaacac
      601 tttgcggcgg aacagcaaat tgataccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaccgct cccagccggc
      721 ccagcaggtt ggctaccggc tcatccgtat cgaacaatgc actgaagccc tgttgagcaa
      781 cggcctggtc ttctttccat gtaaacaggt aagaaataaa tgaaatgaac aagaacactg
      841 caataagcag gaatacggag ccgacaatct tccaggtacg ttcatcacgg gccagtgctt
      901 tcaggtcaat gctttcttct ttttcggccc ggaatggttt tacatcagac ccttcaggtt
      961 ttttcgcagg actttttttc tttttttgct tttcagccat ggcagcaaag ataaaaaagg
     1021 ggaagcagga taaaacggca caattggtaa atctttagcc attcaaaagc ctcccctttc
     1081 gccaatattt acctgaaagt ggtatgtccc ggactggcaa aagcaaaagg gttgtattac
     1141 atttgacgga taagcattcc agcaatgacc aggctatacc gttcatttat ttttcttacg
     1201 atgagcttaa tgctgctggg cagcaaggcc tacacacagg atagcctgca gcaacaattt
     1261 accgacataa gtaagatcag ccttgtcagg caactgtcga aaaccatcaa cagcacttat
     1321 accgataaga atatcgagct ggccaaagaa tatgctcagt taaagttttc accgggaata
     1381 aaacacaaag gaactgttcc caatgaatat gtaacccgaa aactggccct gcaatttaat
     1441 atttccaatt ccgctgattc tactgtttcc gtttacttct ttcctggctt ttattacagc
     1501 agaatcgatc tataccaaaa gacagctacc gggcttgtta atatcccatc ggtattaccc
     1561 tccaacgaag acagtattgg ctaccgccta atttcattag ggggtcatga ttcggctacg
     1621 attatagccc atttgtcctt cgtaaaaacg tatatcaata ctatccggcc aagactgatt
     1681 cataaagatc atattgattc cttcctggca caggtcagaa gcagtcataa taacgataac
     1741 ctggtcactt atgtattttg cggattgctt ttgatgatga tattgttttc cttttctaat
     1801 tttttgcaag gagctaacag ggaattcttt tattattcat tatacgcctt tttgctgggc
     1861 tcaatgctat tcaccaaagc cacttatgat tttcagacca cacatattac tttcttcctg
     1921 gaggcatacc ttgattttat cctgcagggt cttggaataa ttttctacat gatcttcatg
     1981 cagaagttcc ttaatacccg gcaaaatcat ccttttcttt tcaaattcta taatgcagga
     2041 atcatcatgt tagctgtaac cctgttactc tacagttacc tgcatttttt tacagataat
     2101 ttcagcctgg aaaatacagt agaaaatgcg tccaaattgc ttttgctgct gatggttctt
     2161 atttttttag tgtatagcat cagaaactgg aaagacagac tgatgcggta tcttttctgg
     2221 ggtaatcttt gcctgtttct tttttctgtc atttcacagc taagtgtgat gatggaacag
     2281 ctgttcaaag atttacccgg cgtatttagt tcagctttgt tttactacga gataggtctt
     2341 ttcctggaac tggttttctt cctggctggc cttaatcata aaaatcggag atccatcatc
     2401 cagcggacca aagaaaccga aacattgaag gcgcaaaacc tgatgcagca atatgaaaaa
     2461 gaaattgcgg tattcaaagc gcagcaggca gaaagggaaa gaatatcagc cgatatgcat
     2521 gatgaactgg gttccggcat gacggccatc cgcctgatga gtgaaattgc ccggaataaa
     2581 atgaaagaaa atacaccagt tgaaattcac aaaatttctc aatcagccga tgaagtgctg
     2641 aacaagatga atgccatcat ctggagtatg aacagcggca acgacacatt ggacaatctt
     2701 ctttcttata tccgttctta ttcactggaa taccttgaca ataccaacat agactgcaag
     2761 gtgg
//
LOCUS       DAORLS010000372         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19980_length_2761_cov_2.075758, whole genome shotgun sequence.
ACCESSION   DAORLS010000372 DAORLS010000000
VERSION     DAORLS010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19980_length_2761_cov_2.075758"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK977_18455"
     CDS             complement(<1..262)
                     /locus_tag="PK977_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF20169.1"
                     /translation="MKENRQSYSQSKALWAITRASFKAIFANPSAIIFSILFPIIFTL
                     IFGAFGNGSGVTYRIALTPDSDTTSEFYQGLKQTPVISIVSYP"
     gene            324..806
                     /gene="folK"
                     /locus_tag="PK977_18460"
     CDS             324..806
                     /gene="folK"
                     /locus_tag="PK977_18460"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRF20170.1"
                     /translation="MNTAYLLTGGNLGNREENLAAARKLITQHCGTIVHASSLYETAA
                     WGKTDQPPFLNQALELHTELNARQLIRRILKIEKQMGRIREEKYGPRIIDIDILLFNE
                     EIHDYRLLKLPHPEMQNRRFALLPLAEIAPNALHPVFDKTVIELLNECKDKLPVNKYS
                     "
     gene            874..>976
                     /locus_tag="PK977_18465"
     CDS             874..>976
                     /locus_tag="PK977_18465"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRF20171.1"
                     /translation="MNYHFITIEGNIGAGKTTLAHLLRKHYNARLILE"
     assembly_gap    977..1002
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1003..1517
                     /locus_tag="PK977_18470"
     CDS             <1003..1517
                     /locus_tag="PK977_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRF20172.1"
                     /translation="FLPKFYENPQQYAFPVELFFMAERYKQLKELIQQKDMFQSVTIS
                     DYLFTKCLLFSKVTLPDDEFRLYQRLFDIIHQQLIQPDILIYLHAPVSKLQANIKKRN
                     RSYEQNIPDDYLFNLQETYTHYIKQHNVKTLFVDASNADFLGNEKHLQAIYDALEKEY
                     PDGQHYITLP"
     gene            1514..2758
                     /locus_tag="PK977_18475"
     CDS             1514..2758
                     /locus_tag="PK977_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF20173.1"
                     /translation="MNGKFSIKKPFSTVINQEFTWFIISRILFIAGLRMTPVLLGWRL
                     YQITGSKLALGVLGLSEVIPAILLALPAGVKVDKSNKHRLISICIALYFFIMTGLLVV
                     TSGWMQENYSRAVIEWSIYLLVAGTGAVRAFTGSAFNAFLAQLVPSESLVRAVSINSM
                     VWLIAAVAGPAIAGILMGYTNITIAFIPVCVLVGIAFFLFQKIKPKPVSWQGSSKTWD
                     GVKEGLQFVFRQKALLGAMSLDMFAVLFGGAVALLPAFANDILHVGPQGLGWLVAATY
                     MGNFIAIAWLTSHPLKGRQGKTLLYVVAGFGLCILVFAISRNFWLSFAALFASGLFDG
                     VSVIIRGTIVQLFVPDEMRGRVSSVNSIFINSSNELGQFESGVAATAMGMVPSVIFGG
                     CMTLLVVIVTWFKAPGLRKFEY"
BASE COUNT          772 a          574 c          594 g          795 t
ORIGIN      
        1 cgggatagct gacaatgcta atgaccggtg tttgtttcag tccctggtaa aattcgctgg
       61 tagtatcact gtcgggtgtc aaagcaatac gatatgtaac gccgctgcca tttccaaagg
      121 caccgaatat caacgtaaag atgatgggaa aaagaatact gaaaataatg gcacttggat
      181 tcgcaaaaat ggccttaaaa cttgcccggg taatggccca caacgccttt gactgactgt
      241 aagattgcct gttttctttc atcaggcagc aaatgtattt aattactgtt tcaatcctaa
      301 aataaagata tttgagccct tatatgaaca cagcctattt acttacaggt ggcaaccttg
      361 gcaaccggga agaaaacctt gctgccgcca gaaaactgat cactcagcac tgtggtacta
      421 tcgttcatgc ctcatcattg tatgaaacag cagcatgggg aaagacggac cagcccccat
      481 ttttaaacca ggctctggaa ctccatactg aacttaatgc ccgtcaactc attcgaagga
      541 tcctaaaaat tgagaaacag atgggacgta tacgggaaga aaaatatgga ccccggatca
      601 ttgatattga tattctatta ttcaatgagg aaatacatga ttaccggtta ttaaaattgc
      661 cgcacccgga aatgcaaaat cgccgatttg cattgctgcc gctggctgaa attgcaccta
      721 atgccctgca cccggttttc gataaaacag ttattgaatt gctgaacgaa tgcaaagaca
      781 aactgccggt aaacaagtat tcctgatttt ttccacaatc ttttaggttt aagccataca
      841 cctttgcatt taaagtattt tgcgtccctg cgcatgaact atcattttat caccatagaa
      901 ggtaatatcg gggccggcaa aaccactctt gcccacttgc tgagaaagca ttacaatgcc
      961 cgcctgatac tggaagnnnn nnnnnnnnnn nnnnnnnnnn nnccttttta cccaagtttt
     1021 atgaaaaccc ccagcaatat gcattcccgg tagagttatt ttttatggca gaacgttata
     1081 agcaattaaa agaacttatc cagcaaaagg atatgtttca aagtgtgacc atttcagact
     1141 acctgtttac caagtgcctg ttgttttcaa aagtcacctt gccggatgat gaattcaggc
     1201 tataccagcg attgtttgat atcattcacc agcaactgat acaacctgat atacttattt
     1261 acctgcatgc accggtctcc aaactgcagg ccaatatcaa aaaaagaaac cgctcttacg
     1321 aacagaatat ccctgacgat tatcttttta acctgcagga aacctatacg cattatatca
     1381 agcagcataa tgtaaagaca ttatttgtcg atgccagcaa tgccgatttt ttgggtaacg
     1441 aaaaacactt gcaggcgatc tacgatgcat tggaaaaaga atacccggat ggccagcatt
     1501 atattacgtt gccatgaacg gaaaattttc tataaagaaa ccttttagta ccgttatcaa
     1561 ccaggaattc acctggttta ttatttcccg tatacttttt attgccggat tgagaatgac
     1621 acccgtactg ttgggttggc ggttatacca gataacaggc agtaaactgg cactgggtgt
     1681 tttgggactt tctgaagtaa tcccggcaat attactggca ttgccagccg gtgtaaaagt
     1741 ggataagagt aacaagcacc gcttgataag catctgcatc gcactttact tttttattat
     1801 gaccggcctt ttggtagtaa cctccggctg gatgcaggaa aactattcga gagctgtgat
     1861 agaatggtcc atctatttat tggtagccgg aaccggggcg gtaagggcat ttacaggctc
     1921 agcctttaat gcctttttag cacaattagt accctctgaa tcattagtaa gagctgtttc
     1981 tataaacagt atggtatggc tgatagcagc tgtggcgggg cctgctatag ccggcatttt
     2041 gatgggctac actaatataa caattgcttt tataccagta tgtgtactgg tgggtattgc
     2101 tttctttctt tttcaaaaaa taaaacctaa acctgttagc tggcagggta gcagtaagac
     2161 atgggatggc gtaaaggagg gcctgcaatt tgttttccgg caaaaagcat tactgggtgc
     2221 gatgagcctt gatatgtttg cagtactttt tggcggcgct gttgcattat taccagcttt
     2281 tgctaatgat attctacatg taggccccca gggattgggc tggctggtgg cagccactta
     2341 tatgggaaat tttatcgcca ttgcctggct tacctcacat cccttaaaag gcagacaagg
     2401 taaaacgtta ttatatgtag ttgccggatt tggactttgt atactggtat ttgccatttc
     2461 aaggaacttc tggctaagtt ttgctgcatt atttgccagc ggcctttttg atggggtaag
     2521 tgtaatcata cgtggcacca ttgtgcagtt atttgtacca gacgagatgc ggggccgtgt
     2581 ttcatctgtc aactccatct tcatcaattc ttccaacgag ctggggcaat ttgaaagcgg
     2641 cgtagctgcc acggcaatgg gcatggttcc ttcagtgatc tttggcgggt gtatgacctt
     2701 acttgttgta atagttacct ggtttaaagc gccgggatta cgtaaattcg agtattgaaa
     2761 g
//
LOCUS       DAORLS010000373         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_19981_length_2761_cov_1.934220, whole genome shotgun sequence.
ACCESSION   DAORLS010000373 DAORLS010000000
VERSION     DAORLS010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19981_length_2761_cov_1.934220"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..765)
                     /locus_tag="PK977_18480"
     CDS             complement(22..765)
                     /locus_tag="PK977_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20174.1"
                     /translation="MLLRVKLLYLFCLLSLTVNAQKDQWLRAFPITSYIIDGNDSVKI
                     VQLEMPDDIKVKEKQLGIVQGIYETKHSDTVRKGYGRCHLIKGNYYYFAITGNNSGEP
                     LKGGDLIYTLMDKSNIYYAQAAKLAGHFIRLQSVYEEPFYDRYLIFSKWSEEDEKNLI
                     DSIITDIRFTGNYFLENDPSMDKEITSGIFRGRRVLSVMKDCQEKDVINFLDYIIARP
                     RLYAGKTWKVSEIFATWASEGAPTVERID"
     gene            942..>1909
                     /gene="pfkA"
                     /locus_tag="PK977_18485"
     CDS             942..>1909
                     /gene="pfkA"
                     /locus_tag="PK977_18485"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRF20175.1"
                     /translation="MEKKVSKIGVMTSGGDSPGMNAAIRAVVRTGLYHGLEVYGIMRG
                     YQGLIEDDIYKMESRSVANIIQRGGTILKTARSKEFMEYEGRKKAYDNLKKRGIDGLV
                     IIGGDGSFRGAVKFSQEFDIPCVGIAGTIDKDILGTDFTIGFDTAVNTAVEAIDKVRD
                     TMDAHDRIFIIEVMGRDAGYIALHSGIATGAENILIPERKTDINDIIASLKEKERRKK
                     MVNLIVVAEGDDFGGANEVQKVLQENLPKAEIRVCILGHIQRGGSPSCIDRLIASRMG
                     YHAVESLIEGRHNIFVGIQNNKMNYMPLENAVKNKGKVSDEWMKIV"
     assembly_gap    1910..1951
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1961..>2761
                     /locus_tag="PK977_18490"
     CDS             1961..>2761
                     /locus_tag="PK977_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRF20176.1"
                     /translation="MSKNIEKYLHKHFDNEAGRKHAFKRTKIVATVGPACDTYEKLLE
                     LVKAGVNVFRLNFSHGSHEDKAKIIDYIRQINKKEPYNIAILADLQGPKLRVGDLEGG
                     SLEIKEGDILTFTNEKVVGNKERIYVSYPNLHQDVKVGEKILIDDGKLEVIVTEITDR
                     QEVKVKVLLGGILYPKKGVNLPDTHISLPSMTEKDLADLDFILQHKIDWVALSFVRHP
                     DDITDLKERIVKAGCLSKVIAKIEMPGAIKNLRDIIVESDGVMIARGDL"
BASE COUNT          863 a          497 c          583 g          776 t
ORIGIN      
        1 ataaaaacaa tgttaaaatt attaatcaat tctttctacc gttggcgccc cttccgaagc
       61 ccaggtggca aatatttcac ttactttcca tgtcttgccc gcataaagtc gtggccttgc
      121 aattatataa tcaaggaaat tgatcacatc tttttcctga caatccttca ttacagataa
      181 tacccgtctc cccctgaaaa taccagatgt aatttccttg tccattgagg gatcattttc
      241 aagaaagtaa ttaccggtaa atcttatatc ggttataatt gaatcaatca gatttttttc
      301 atcttcttca ctccatttac taaagatcag gtagcggtca taaaatggtt cttcatacac
      361 tgattgtaat cggataaaat gtcctgctag ttttgcagcc tgggcataat atatattgga
      421 tttatccatc aatgtataaa taaggtctcc ccccttcagc ggctctccac tattattacc
      481 tgtgatggca aagtaataat agttgccctt aatcagatga catcgtccat atccctttct
      541 tactgtatca ctatgctttg tctcataaat accctgaact attcctaatt gtttttcttt
      601 tactttgatg tcatccggca tttccagttg tacaatctta acactgtcgt taccatctat
      661 aatataactg gtaatcggaa aggcacggag ccattgatct ttttgtgcat ttactgttaa
      721 tgacaaaaga caaaacaggt acagcagctt aactcgtaaa agcatagtcg ttatttttat
      781 ctaacctatt gacaaatatt aaaaccagca taccctaaag ctggtattta tggaaatgat
      841 tgcttgcctg agagaggtta tatttgccag aagctaacga atggttgtgt ataaaagaca
      901 caatcagaac aaaaccctac ttttgctgtt cataattaat aatggaaaag aaagtaagta
      961 aaatcggagt tatgacatcc ggtggagatt cccccggcat gaatgctgcc atccgggccg
     1021 tagtaagaac tggtctttac catggccttg aggtatatgg aattatgagg ggctaccagg
     1081 gcttgattga ggatgatatt tataaaatgg aatcaaggtc tgtcgccaat atcattcagc
     1141 ggggtggtac aattttaaaa actgcccgga gcaaagaatt tatggaatat gaaggccgga
     1201 aaaaggctta tgataacctg aaaaaacggg gtattgacgg gctggtaatc attggaggtg
     1261 atggctcttt ccgtggtgct gtcaaattca gccaggagtt tgacattccc tgtgtgggta
     1321 ttgccggtac aatcgataag gatatccttg gaactgattt cacaatcgga tttgatacgg
     1381 cagtcaatac ggctgttgaa gccattgaca aggtaagaga tacgatggat gcccacgaca
     1441 ggatctttat catagaagtt atgggccgtg atgcaggtta tatcgcactg cacagtggca
     1501 ttgccaccgg agctgaaaac attcttattc cggaacgaaa gacggatata aatgatatta
     1561 ttgcctccct taaggaaaaa gaacgtcgta aaaaaatggt caacttgatt gtggtagcag
     1621 aaggtgatga ctttggaggt gcaaatgaag tacaaaaagt gttacaggaa aacttgccca
     1681 aagctgaaat aagggtttgt attttggggc atattcagcg gggaggttca ccaagttgca
     1741 tcgacaggtt aatagccagc cgcatgggtt atcatgcggt tgaaagcctg atagaaggac
     1801 ggcataatat cttcgtcggt attcagaata ataagatgaa ttatatgccg ctggaaaatg
     1861 ctgtcaagaa taagggtaaa gtcagtgatg aatggatgaa gattgtcaan nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngattattat atgagcaaga atattgaaaa
     1981 atatctccat aaacattttg ataatgaagc cggccgaaaa catgctttta aacggacaaa
     2041 aattgtagcc acagttggcc ccgcctgcga cacttatgag aagctgctgg aactcgttaa
     2101 agccggagta aatgtattca ggctcaactt ttctcatggc agtcatgaag acaaagcaaa
     2161 gattattgac tatatacggc agatcaataa aaaggagcct tataacatag ctatcctggc
     2221 cgatctgcaa ggccctaaac tcagagttgg tgatttggaa ggaggttcat tagaaattaa
     2281 agaaggagat atcctcacat ttacgaatga aaaggtagta ggcaataagg agaggattta
     2341 tgtctcctac cctaatctgc accaggatgt aaaggttggt gaaaaaatac tgatagatga
     2401 cggaaagctg gaagttattg taacagagat aactgaccgg caggaagtaa aggtgaaagt
     2461 gcttttaggg ggtatcctgt atcccaaaaa gggtgtaaac ctccccgata cacatatttc
     2521 tcttccgtct atgacagaaa aagatcttgc tgatcttgat tttatacttc agcataaaat
     2581 tgattgggta gctctttctt ttgtaaggca ccctgatgac ataactgacc taaaagaaag
     2641 gatagtaaaa gcaggctgcc tttcaaaagt gattgcaaaa attgagatgc ccggagctat
     2701 aaaaaacctt cgtgacatta tcgtagaaag cgacggtgta atgattgccc gtggtgatct
     2761 t
//
LOCUS       DAORLS010000374         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20260_length_2727_cov_1.910180, whole genome shotgun sequence.
ACCESSION   DAORLS010000374 DAORLS010000000
VERSION     DAORLS010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20260_length_2727_cov_1.910180"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1502)
                     /locus_tag="PK977_18495"
     CDS             complement(186..1502)
                     /locus_tag="PK977_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF20177.1"
                     /translation="MIKIFVFSATLLLSLLSIAQRTTENDGDWSLQFVVLKNTVEADL
                     MIRVGDIDNLGFGFEEAFNPFSGRSTWSHGFPWDIKKDDAKGTDRIMVPSSYKYGTWV
                     SQDGYTSSTQRPSNQPMTIAIPLAELKGVKVDSASLQLFIDDFQAPALNSKFQVKING
                     LRFTEAEKLINRVNQTGPVGKLITIRLTPELLEKLKADSLVIAIDDPVSKQGDGYAID
                     FVKLLINPKVIYQGKVQGRIVDAATKEPIAGATAVIEDYGTAITDNEGNFIIENVPAG
                     LSIVSGSAAGYSSETKQVDVIADESSELVELELKRSGKVTFNNKALQEGDNLVLNNIQ
                     FEVNSATLLAAGRAELDKLAAFMKQNVGIEILLSGHTSSEGGAALNRELSLKRVRSCK
                     NYLVGKGIEDGRITIKGYGPDMPIAPNDTEVNRAKNRRVEMKITRL"
     gene            1607..2296
                     /locus_tag="PK977_18500"
     CDS             1607..2296
                     /locus_tag="PK977_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF20178.1"
                     /translation="MAEAIIELKHVNIYQGNNLVLQDVNLTVNKGEFVYLVGKTGTGK
                     SSLLKTLYGELSLTEGEGMVAGFSLRGLDWKKVPYLRRNLGVVFQDFQLLTDRNVNDN
                     MKFVLKATGWTDEKLMDEKIMDVLDKVGLKAKGFKMPFELSGGEQQRIDIARALLNSP
                     KLILADEPTGNLDPETSDEIMNLLFSISKDYNTAIVMATHDYIVVQKFPARMIRTERG
                     RVVDNASINIE"
     gene            complement(2277..>2727)
                     /locus_tag="PK977_18505"
     CDS             complement(2277..>2727)
                     /locus_tag="PK977_18505"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF20179.1"
                     /translation="ATWLLEKVFSKIKVPFVVAGKNPSKKLETLAHLYLHTCIVANPS
                     DTEINDLIQKAHINILPSFSDTGIKIKLLHALFEGRHCVVNEAMVQGTGLEAACHIGA
                     NADAFAAIIMQLHHLPYAEEETKLRKRLLGDLYNTASNARQLIQYLY"
BASE COUNT          739 a          553 c          602 g          833 t
ORIGIN      
        1 gtaaagccgg aaactgttgg gttagtcaac tgcattcaaa tgtagagaat tccggaaatt
       61 ggtggttcct ccgcaaataa aatattttat taaatatagt tattaattga tttataacaa
      121 tttaaacgaa tttatttgaa tagtagttta gaaaattatg taccagaata ctcttaataa
      181 attcattaga gccgggttat tttcatttct actcgccggt ttttggccct gttgacttca
      241 gtatcgttgg gcgcaattgg catatcgggc ccatagcctt taatggtaat cctgccatct
      301 tctatgcctt ttccgacaag gtagttcttg caacttctta ctcttttcag cgaaagctcc
      361 cggtttagcg ctgcaccacc ttcggaggat gtatggcctg acaataagat ctcaatgccc
      421 acattctgtt tcataaaagc cgccagttta tccagttcag ctctgccagc tgccagtagt
      481 gtggcgctgt tgacttcaaa ctggatattg ttaagcacca gattatcgcc ttcttgtaac
      541 gctttattat taaaagtgac tttgccagaa cgtttcagct ccagttctac caattcgctg
      601 gattcgtcag caattacatc aacctgtttg gtttccgaag aatatcctgc agcactgccg
      661 cttacaatgg ataaaccagc cggtacattc tctatgataa aatttccttc gttatctgta
      721 atggccgttc cataatcttc aattactgca gtagccccgg caatcggttc ttttgtcgca
      781 gcgtcaacga ttcttccctg taccttaccc tggtaaatga ctttgggatt gatcagcagc
      841 tttacaaaat caatggcata accatcacct tgtttggaaa caggatcatc tatagcaata
      901 accaatgaat cggcttttag cttttccagc aattctggtg tgagtcggat tgtgattagt
      961 ttgccgacag gaccggtttg attaacccgg ttaatcagtt tctctgcttc cgtaaaacgc
     1021 aggccgttga tctttacctg gaattttgaa tttaaagccg gggcctggaa atcatcgatg
     1081 aataactgca gggaagctga atcaactttt actcccttga gttcagccaa cggtatggcg
     1141 atggtcattg gttgattgga tggccgctgg gtagaactgg tgtagccatc ctgtgatacc
     1201 catgtaccgt atttatagct tgacgggacc attattcggt ccgtgccttt tgcatcatct
     1261 ttttttatat cccagggaaa gccatgcgac caggtggaac ggccggagaa gggattgaat
     1321 gcttcttcga atccgaaacc caggttatca atatcaccca cccgtatcat caggtcagct
     1381 tctaccgtgt tttttaaaac aacaaattgc aggctccagt caccatcatt ctccgttgta
     1441 cgctgtgcta ttgaaagtaa acttaaaaga agagtagcgg aaaagacaaa tatttttatc
     1501 atgaagtatt ttttggttta atgctatagc taagatacaa gtgttaatga tttttttgag
     1561 tttgtcttcc tattctttcc tttactttgc agcaaactgg caatcaatgg ctgaagcgat
     1621 catagaatta aaacatgtca acatttacca gggtaataac ctggtgttgc aggatgtgaa
     1681 cctgactgtg aataaagggg agtttgttta cctggtaggt aaaaccggaa ccggtaaatc
     1741 gagtttactg aaaacactat acggcgaact ttcgctgacg gaaggagaag gtatggtggc
     1801 cggtttcagt cttcgggggc tcgactggaa aaaagtgcct tacctgcgtc gtaaccttgg
     1861 cgtggtattc caggattttc aattgcttac tgaccggaat gtaaacgaca atatgaaatt
     1921 tgtattgaag gctaccggct ggacggatga aaaactgatg gatgaaaaga ttatggatgt
     1981 gctggataaa gtggggttaa aggcaaaagg atttaaaatg ccctttgagc tgagcggtgg
     2041 cgaacaacag cgtattgata ttgcccgggc tttattgaat tcacccaaac tgatactggc
     2101 cgatgagcca acaggtaacc ttgatcctga aacttctgac gagatcatga acctgctgtt
     2161 cagcatctct aaagattata acacagccat tgtaatggcc actcatgatt atattgtcgt
     2221 acagaaattc ccggctagaa tgatccggac ggaaagggga cgggttgttg ataatgctag
     2281 tataaatatt gaataagctg gcgggcattg gatgcagtat tgtacaggtc gcccaataat
     2341 ctttttctca atttggtttc ttcttctgca taaggcaggt ggtggagttg cattataata
     2401 gctgcaaatg catcagcgtt ggcaccgata tgacaagctg cttccagccc ggtgccctgt
     2461 accatggctt cgttgaccac acaatgtctt ccttcgaata aagcatggag taattttatt
     2521 ttaattccgg tatcactaaa ggaaggaagg atattgatat gtgctttctg aataaggtca
     2581 ttgatctctg tatcagaagg attggctacg atacaggtat gcaggtacag gtgtgccagc
     2641 gtttccagtt ttttagaagg attcttgcct gctactacaa agggtacttt tattttagaa
     2701 aatacttttt ccagcagcca ggtagca
//
LOCUS       DAORLS010000375         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20354_length_2717_cov_1.942149, whole genome shotgun sequence.
ACCESSION   DAORLS010000375 DAORLS010000000
VERSION     DAORLS010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20354_length_2717_cov_1.942149"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>237
                     /locus_tag="PK977_18510"
     CDS             <1..>237
                     /locus_tag="PK977_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF20180.1"
                     /translation="VLFRFIFGAGTICNNYDGFAIDDIVITEAPANDASFTYSCVNSR
                     TVNFTNTSALCPVTYSWNFGDPASGSNNTSSIVNP"
     assembly_gap    238..347
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <348..847
                     /locus_tag="PK977_18515"
     CDS             <348..847
                     /locus_tag="PK977_18515"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF20181.1"
                     /translation="LQTVNCQTNTGGSLTVDVTGAAGVPLNLLWNTTPPQTSTIISGL
                     SEGFYNVQISGTNVCPLTATGKAEKDFSCTGIYFPSAITPNNDGRNDGFGPLGSLLSL
                     TDYRLSVYNRWGERVFYTVNPFERWKGKVKGLETDSNIFVWYAEFTIPGKPKETRKGI
                     VVIIR"
     gene            complement(848..>2717)
                     /locus_tag="PK977_18520"
     CDS             complement(848..>2717)
                     /locus_tag="PK977_18520"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF20182.1"
                     /translation="FIRVLNQVRNNELDEEGITILESCYNPTVRRKKDDGFIILTTHN
                     EQARNINVSQLQQLNTPPVQYDALITDDFPENAFPAEETLYLKVGAQVMFIKNDIADR
                     GKRYFNGKIGIVTKLEEEAVFVECDDKEIKVEREKWDNIRYTLDKASQTMKEDVLGSF
                     TQFPLRLAWAITIHKSQGLTFDKVIIDAGEAFAPGQVYVALSRCTSLNGLVLKSKIKT
                     GSLLTDPKIIQFARNSSSSAQLQNELLQAKKEYQLRILVDSFNYHNAINNAVTLKEYV
                     LENIESFKKEAADWIEKLLNQLNTLQETAVKFHGWLQVQFLQPEAPEENKVLLEKTIK
                     ASAHFVNELEIIINSLGNTTLVTDSKLHAKECNELLKDIYTGLSRNKHLITGFSGKID
                     SAAWHQRKRNFVLPSFPVNVYAGASTQKTDSPHPALFVQLKKQRDAFCEKRDLPVYMV
                     ANSQTLDEMARYLPQSPEELKKISGMGEAKIRQYGQPFLDIILEYCHEHGLSSHIQEK
                     TPKKERKQKGGTAKKKGDSHAASFRLFSEGKTIADIAKERKLAISTIEGHLCRFISSG
                     EINIEEVIGKEKATMILEALKDYDRTAIGSAKANLPPEISYGEIRMVVAGMNLLQK"
BASE COUNT          659 a          583 c          522 g          843 t
ORIGIN      
        1 tgtgttattc aggttcattt ttggtgcagg tacaatctgt aataattatg atggttttgc
       61 tattgatgat attgtaataa cagaggcgcc ggccaatgat gcatctttca cttacagttg
      121 tgttaacagc agaacggtca acttcaccaa tacttctgca ctttgtcccg taacatatag
      181 ctggaatttt ggtgacccgg cttccggaag caataacact tcttccatcg ttaatccnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc tgcaaacagt
      361 gaactgtcaa accaataccg gtggctcgtt gacagttgat gtaaccgggg ctgccggggt
      421 gccgcttaac cttttatgga atactactcc gccacaaacc agtacgatca tctcgggtct
      481 ctcagaggga ttttataatg ttcagatatc gggtacaaat gtctgtccgt tgacggcaac
      541 cggtaaagca gaaaaagatt tttcttgtac aggcatttat ttcccttctg caattactcc
      601 caataatgat ggcagaaacg atggctttgg cccattggga agtttattat cattaacaga
      661 ttaccggttg agcgtttata acaggtgggg cgaaagggtt ttttatacgg tcaatccttt
      721 cgaacgatgg aagggaaagg taaaaggact ggaaaccgac agcaacatat ttgtatggta
      781 tgcagaattc accattcccg gcaaaccaaa agaaacaaga aaaggaatag tggttattat
      841 caggtgatta tttctgcaac aaattcatac cagcaaccac cattcttatt tcaccatagc
      901 tgatctccgg cggaaggttt gcttttgcag aaccaatggc tgttctatca tagtctttca
      961 atgcctccaa aatcattgtt gctttttcct taccgattac ttcttcgata tttatttcac
     1021 cgctgctgat gaaccggcac aggtgcccct cgatggtgct aatggcaagc tttctctctt
     1081 ttgcaatatc agcaattgtt tttccctcac tgaataaacg gaaagacgct gcatgactgt
     1141 cgcccttttt cttagccgtg cccccttttt gttttctttc cttctttggt gttttttcct
     1201 gaatatggga agacaaccca tgttcatggc aatattcgag aatgatatct aaaaacggct
     1261 gcccatactg ccggattttt gcctcaccca ttccgctgat cttttttaat tcttccggtg
     1321 attgcggcag gtaacgggcc atttcatcca atgtctggct gttagccacc atgtaaacag
     1381 gcaaatctct tttttcacaa aaagcatccc gctgtttttt caattgcacg aataaagcag
     1441 gatgagggct gtctgttttt tgtgttgatg caccggcata cacattaacc gggaaggaag
     1501 gtagtacaaa gtttcttttg cgctgatgcc aggctgcgct gtcaattttt ccggaaaacc
     1561 cggtaatcaa atgtttattt cttgaaagcc cggtataaat atccttcagc aactcgttgc
     1621 actcctttgc atgcagcttg ctgtcggtaa caagagtagt atttcccaac gaattaatta
     1681 ttatttctaa ttcatttaca aaatgtgcgg aagctttaat tgttttttcc agcaagactt
     1741 tgttttcttc cggagcttcc ggttgcagaa actgaacctg aagccagcca tgaaatttaa
     1801 cagctgtttc ctgaagcgta ttgagttggt tcagcaattt ttcaatccag tctgccgcct
     1861 cttttttgaa tgattctatg ttttccagca catattcttt cagggtaaca gcattgttta
     1921 ttgcattgtg ataattaaac gaatcaacca gtattctcaa ctggtattct tttttggcct
     1981 gcaacaattc attttgtaat tgtgcagagg aagaactgtt gcgggcaaat tgtatgatct
     2041 tggggtcggt caacaaactg ccggttttta ttttactttt cagcactaac ccgtttaaac
     2101 tggtgcaacg gctcagcgcc acatacacct gccccggtgc aaaagcctca ccggcatcaa
     2161 tgatcacctt atcaaacgta agaccctggc ttttgtgaat ggtaattgcc caggccagcc
     2221 gtaagggaaa ctgggtaaaa cttccgagta catcctcttt catggtttgc gatgctttat
     2281 ccagggtata gcggatatta tcccattttt ctctctccac ttttatttct ttgtcatcac
     2341 attcaacaaa gacagcctct tcttccagct ttgttacgat gccgatcttt ccgttgaaat
     2401 atcttttacc cctgtccgca atatcatttt tgatgaacat tacctgtgcg cccactttca
     2461 ggtaaagcgt ttcttcagcc gggaaggcat tttcgggaaa atcatccgtt atcagtgcat
     2521 catattgaac aggaggtgta tttagctgct gcagttgtga cacattgata ttcctggcct
     2581 gctcattatg tgtggtgagt ataataaatc cgtcatcttt tttccttctc acggtgggat
     2641 tatagcagga ttcaagtatc gttattccct cttcatccag ttcattattc cgcacctggt
     2701 tcagaacccg gataaat
//
LOCUS       DAORLS010000376         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20372_length_2716_cov_1.933484, whole genome shotgun sequence.
ACCESSION   DAORLS010000376 DAORLS010000000
VERSION     DAORLS010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20372_length_2716_cov_1.933484"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK977_18525"
     CDS             complement(<1..216)
                     /locus_tag="PK977_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20183.1"
                     /translation="MKATLLACLFFISFLASAQEDAATLYVTAKDYFLKGEFEKGLPL
                     AEKAVQKAKTQIGETTSDYGTIINLLGL"
     gene            complement(340..>1141)
                     /gene="asnS"
                     /locus_tag="PK977_18530"
     CDS             complement(340..>1141)
                     /gene="asnS"
                     /locus_tag="PK977_18530"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRF20184.1"
                     /translation="FGDIYTFGPTFRAENSNTARHLAEFWMIEPEMAFYDLEDNMNLA
                     EEFIKYIIKYAMENNREDLEFLAQRLAEEEKQLPQDKRSEMGLIEKLEFVLNNSFERL
                     TYTDAIQILKDSNHNKKKKFQYEITGWGMDLQSEHERYLVEKHFKKPVILYNYPAAIK
                     AFYMRQNDGCAEGQQTVAAMDILAPGIGEIVGGSQREERLDKLEARMKEMHIPTEEMW
                     WYLDTRRFGTVPHAGFGLGFERMVQFVTGMTNIRDVIAFPRTPGSAEF"
     assembly_gap    1142..1197
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1198..2226)
                     /locus_tag="PK977_18535"
     CDS             complement(<1198..2226)
                     /locus_tag="PK977_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRF20185.1"
                     /translation="MFNKRIKVKELLAMEPQGQEVTVMGWVRTFRNNQFIALNDGSTN
                     NNIQVVVELGSYDDEFLKRITTSASLKVTGTIIPSLGKGQKMDLKATSIEILGDSDAE
                     KYPLQPKKHSLEFLREKAHLRFRTNTFGSVFRVRHALAFAVHKFFNEKGFVYMHTPII
                     TASDAEGAGEMFRVTTFNLEDVPFKSDRFDTAAENFYNNLIDSIEEEQRTLIIGKINA
                     ILKRKWEDLTFKKIVLGHFGLIQNLAKQKEDFRLLNPYFKILYAIIKNNNFFEIRLSE
                     KTNKRVTYFPTWFFESINPSKKKVSVKRVFGIKDGFDYLCFSYFNFEFVVKELRNSKE
                     RKVDFKEDF"
     gene            complement(2312..>2716)
                     /locus_tag="PK977_18540"
     CDS             complement(2312..>2716)
                     /locus_tag="PK977_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20186.1"
                     /translation="SVSAMIFSFFLLNSNFYPKLLTYQAGKPMAAITKGKLNPEDVWF
                     WKGNYSSSFNFYTKTHRKEFADSVLNINKKVWLAFDTRVEKEITGAGYKLGQRYSTKD
                     YEITKLDIKFINPEKREKECSEMVIAEVLRRQ"
BASE COUNT          708 a          588 c          485 g          879 t
ORIGIN      
        1 caagcccagc aggttgatga tggttccgta atcggatgtg gtttcgccaa tttgtgtttt
       61 agctttttgt actgcttttt ccgccagcgg aagtcctttt tcaaattcac ctttcagaaa
      121 ataatctttg gctgtaacat aaagcgttgc ggcatcttcc tgggcggatg ccaaaaaaga
      181 aataaaaaat aagcatgcca gcagcgttgc tttcatgagg agtttaatga ttgcctgttg
      241 aatttacgca atcttatgca ggttcataat attctatagc taaccccaaa aaattaaagg
      301 tgccgtttga gcacctttaa caatctaatc agtaagtcat caaaactccg cactgcccgg
      361 cgttctcgga aaagcaatga cgtctcgaat attcgtcatg ccggttacaa actgtaccat
      421 gcgttcaaaa cccaaaccga aacctgcatg cggtacggta ccaaagcgtc ttgtatccaa
      481 ataccaccac atttcttcag tgggtatatg catttctttc atccttgctt ccagtttatc
      541 cagccgctct tctctctgtg aacctccaac gatctcacca atgcccgggg ccagtatatc
      601 catggccgcc accgtttgct gtccttcagc gcaaccatca ttctgccgca tgtaaaaggc
      661 tttgatagct gcaggatagt tatagagtat aaccggcttc ttaaaatgtt tttctaccag
      721 gtaacgttca tgctcactct gcaggtccat gccccagccg gtaatctcgt actggaattt
      781 tttcttcttg ttatgattgc tgtcttttaa aatttgaata gcatcagtat aggtcagtct
      841 ttcaaagctg ttgttcagca caaactccag tttttcgatc agccccattt cactgcgctt
      901 gtcctgcggc agttgctttt cttcttctgc taaacgttgt gcaaggaatt ccaggtcttc
      961 ccggttgttt tccatcgcat actttattat gtatttgatg aactcttcag caaggttcat
     1021 attgtcttca aggtcataga aggccatttc gggttctatc atccagaact cggccaggtg
     1081 ccttgccgtg ttgctgttct ctgcccggaa agtaggacca aatgtataga tatcgccaaa
     1141 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     1201 atcttctttg aaatcaactt tcctttcttt tgaatttcgt aattctttaa cgacaaattc
     1261 gaaattgaaa tagctaaaac ataaatagtc aaacccatct tttattccaa acaccctttt
     1321 cacagatact ttcttttttg atggattgat actttcaaaa aaccaagtcg ggaaatatgt
     1381 aactctttta tttgtttttt cacttaagcg tatttcaaaa aaattattgt ttttaataat
     1441 tgcatatagt attttgaaat atgggtttag taaacgaaag tcttcttttt gttttgctaa
     1501 gttttgaatg agcccaaagt gtcctaaaac aattttttta aaagttaagt cttcccattt
     1561 tcttttcaat attgcattaa ttttacctat aataagggtt ctctgctctt cctctataga
     1621 atcaataaga ttattataaa aattttctgc agcagtatcg aatcgatcac ttttgaaagg
     1681 aacatcctcc agattgaatg tggtaaccct gaacatttca ccagctcctt ctgcatcact
     1741 ggcagtgata atgggtgtgt gcatatacac aaaccctttt tcattgaaga atttgtgaac
     1801 cgcaaatgcc agtgcatgac gtacacgaaa aaccgaaccg aatgtattgg tacgaaaacg
     1861 caggtgtgct ttttcccgta aaaactcaag actgtgtttc ttgggctgca gcggatattt
     1921 ttcggcgtca ctatcaccca gtatttcaat gctggtagct ttcagatcca tcttctgtcc
     1981 ctttccgagt gaggggataa tggtacctgt tactttcaga gaagctgaag tagttattct
     2041 tttgagaaat tcatcatcat agcttccaag ctccaccacc acctgtatat tattattggt
     2101 gctgccgtcg ttcaaggcta taaactggtt gttgcggaaa gtccgcaccc atcccattac
     2161 tgttacttcc tgtccctgcg gctccatggc cagcagctcc tttactttga tccgcttgtt
     2221 gaacattatt gaaattttgg tgggcaaaga taacattcaa tggccgaaat gccgccactg
     2281 gtaaggaatt caaacaacgg gtagtcattt attattgcct tcttaaaact tctgcaatca
     2341 ccatttcaga acattctttt tcccgttttt ccgggttaat gaatttgata tccagtttgg
     2401 tgatttcgta gtcctttgta ctgtaccgct ggcccagttt ataaccggca ccggtaattt
     2461 ctttttccac cctggtatca aacgccagcc atactttctt attaatgtta agtacggagt
     2521 cagcaaattc tttacgatgg gtttttgtat aaaaattaaa cgaagagctg tagttccctt
     2581 tccagaacca aacatcttcc gggtttagtt tcccttttgt tatagctgcc attggttttc
     2641 cagcctggta ggtcagcaac ttcgggtaaa aattactgtt gagcagaaag aaagaaaata
     2701 tcatagccga aacgga
//
LOCUS       DAORLS010000377         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20449_length_2706_cov_1.430781, whole genome shotgun sequence.
ACCESSION   DAORLS010000377 DAORLS010000000
VERSION     DAORLS010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20449_length_2706_cov_1.430781"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PK977_18545"
     CDS             <1..732
                     /locus_tag="PK977_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF20187.1"
                     /translation="LYTVLRSKEYAPSDPNWKESLKKEKESGASAGIIKEIASAVAHM
                     PSQMKRLAIVQFLTWPGLFLMWFYYSTGVAADIFQGDAKTNAEVYTKGLEHANNTSAI
                     LNLVTFAFSFTIPFWVGFIGKKFTHTACLLIGGIGLISVNFIHEPWMLYISMSMVGIA
                     WASILSMPYSMLAGAIPQEKMGIYMGIFNFFIVLPEIIASLFFGKIMDSFLNNDRLAA
                     VLIGGVLLCTAGIICALIVKEKKAN"
     gene            737..>2325
                     /locus_tag="PK977_18550"
     CDS             737..>2325
                     /locus_tag="PK977_18550"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF20188.1"
                     /translation="MKKVFLSILLIIVFRVYAQDMPGIYPAHWWTGMKNPNLQLIIHR
                     DRVAMEKISMLPYAGVKLVKQSKAENPNYLFIDLQISASAKPGKIKFRIDNLQSPESV
                     SYKIVEYELKPRSRDNGKTRIKGVNATDFIYLLMPDRFANGDTRNDVIEEYRDKVCDR
                     KDKFSRHGGDFKGIEDKLDYLDQLGVTTLWLTPVIENDMPKMQEWGNQVAGYHGYWFT
                     DHYAIDKRFGGNEGYKKFCSTLHARGMKVIQDAVYNHVGNHHWFALDPPMKSWFNTSQ
                     GDKGPNHREEVFYDPYASGHDKTQMLEGWFVPHLPDLNQRNPYVANFLIQHAIWTTEE
                     YGIDGWRVDTYKYCDELFMNNVNAALEREFPTVTVFGESWVNSTVANAYFTQNNIAAP
                     FKHNARGMLDFQTCFAMLAGMNEPFGWQNGTAKIYTTLTQDVLYRNPLNNCTFLDNHD
                     MDRVFSTVNEDWKKLKMGINWLLTIRGIPQLYYGTEVLMKNKKEGSDAPVREDFPGGW
                     PDDKPKDNRFTKEGRSEKQNEA"
     assembly_gap    2326..2387
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2388..2637
                     /locus_tag="PK977_18555"
     CDS             <2388..2637
                     /locus_tag="PK977_18555"
                     /inference="COORDINATES: protein motif:HMM:NF021922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HRF20189.1"
                     /translation="TTGKTMQFVPKDNLYLYFRYDAKQTVMVITNTGDKAVKPDWKPF
                     NERINGFTKARNVITSKSQPLDGLEINAGESLVLELTR"
BASE COUNT          807 a          503 c          618 g          716 t
ORIGIN      
        1 ttatatactg ttttacggag caaagaatat gctccctctg atccaaattg gaaagaatcc
       61 ctaaaaaaag aaaaagaaag cggtgcatct gcaggtatta taaaggagat agcttcagcg
      121 gtggctcaca tgccgtctca aatgaaaaga ctggccatcg ttcagtttct tacatggcct
      181 ggactttttt tgatgtggtt ttattatagt acaggagttg ctgcagatat ttttcagggc
      241 gatgcaaaaa caaatgcaga ggtttacaca aaaggactgg agcatgccaa taatacttcg
      301 gcaatattaa accttgttac attcgctttc tcgtttacca ttcctttttg ggttggtttt
      361 atcggtaaaa aatttaccca taccgcatgt ttgctcatag gaggtattgg attaatttct
      421 gtaaacttta tacatgagcc atggatgctg tatatttcaa tgagtatggt cggaattgca
      481 tgggcaagta ttttatctat gccatattct atgctcgcag gggctatacc acaggagaaa
      541 atgggcatat atatgggtat ttttaatttc ttcatcgtac tgccagagat tattgcatcg
      601 cttttctttg gaaaaatcat ggatagtttc ttgaacaatg acaggctagc agcggttttg
      661 attggaggtg ttttattatg tacggcggga attatttgtg cattaattgt aaaagagaaa
      721 aaagcaaatt aatcacatga aaaaagtatt tctttctatt cttttaataa ttgtatttag
      781 agtttatgcc caggatatgc ccggtattta tccggcacat tggtggaccg gcatgaaaaa
      841 tcccaacctt cagttgatta tccatcgtga cagggtggct atggaaaaga tcagcatgct
      901 gccgtatgca ggagtgaaac tggtaaaaca atcaaaagcg gagaacccga attatctttt
      961 tatcgattta cagataagtg cttctgctaa acccggtaag atcaaattca ggatcgataa
     1021 cctgcaaagc ccggaatccg tttcttataa aatagtggaa tacgaattaa agccccgcag
     1081 cagggacaac ggaaagacaa gaataaaggg agttaacgca accgatttta tttatctgct
     1141 gatgcccgac cgctttgcca atggtgatac ccgtaatgat gtgattgaag aataccggga
     1201 taaagtttgt gaccgcaaag acaagttctc ccgtcacggg ggcgatttta aaggaattga
     1261 agataaactg gattacctgg atcagttggg cgtaaccact ttatggctga cacctgtgat
     1321 agaaaatgat atgccgaaaa tgcaggagtg gggcaatcag gtggccggct atcatggcta
     1381 ttggttcact gatcactatg ctattgataa acgctttggt ggcaatgaag gatataaaaa
     1441 attctgcagt acccttcatg ccagaggcat gaaagtgata caggatgccg tgtacaatca
     1501 tgtgggcaat catcactggt ttgcccttga tcccccgatg aagagctggt tcaatacctc
     1561 gcagggagat aaggggccta atcaccggga agaggtgttc tatgacccct atgcttcagg
     1621 tcacgataaa acacaaatgc tggaaggctg gtttgtgccg cacctgccgg acctgaacca
     1681 gcgaaatcct tatgtagcca attttctgat tcagcatgcg atatggacca ccgaggaata
     1741 tggtattgac ggctggcggg tagataccta taaatattgt gatgagctgt ttatgaataa
     1801 tgtcaatgcg gctctcgaaa gagaatttcc aaccgttacc gtatttggtg agtcgtgggt
     1861 aaacagcact gtggccaatg cctattttac ccaaaataat attgctgctc cctttaagca
     1921 taatgcaaga ggcatgctgg attttcaaac ctgctttgcc atgctggccg gaatgaatga
     1981 accctttggt tggcaaaacg gtacagcaaa gatttatacc accctgacac aggatgttct
     2041 ttacaggaac ccactgaata actgtacatt cctcgacaac catgatatgg acagggtatt
     2101 ttcaacagtg aacgaggatt ggaagaaact aaagatggga atcaattggc tgctgacaat
     2161 aagaggtata ccgcaattgt attacggaac ggaagtatta atgaaaaaca aaaaagaagg
     2221 atcggatgca ccggtaaggg aagactttcc tggtggctgg ccggatgaca aacccaaaga
     2281 caaccggttt accaaagagg gcaggagcga gaaacagaac gaagcnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac taccggtaaa
     2401 acgatgcagt ttgttcccaa agacaatctc tacctttatt ttcgttatga tgctaagcaa
     2461 acggtgatgg tgattaccaa taccggtgat aaagcagtga aacccgactg gaagccattt
     2521 aacgaacgga taaatggttt tacgaaggca agaaatgtaa ttaccagtaa gagccagcca
     2581 ctggatggat tggagatcaa tgccggtgaa agccttgtgc tggaattaac caggtaatta
     2641 caggaagata ttccagatgg cctgctggcc ccaattgccc cagtcactgg tcgctttttt
     2701 tacatt
//
LOCUS       DAORLS010000378         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20457_length_2705_cov_1.767925, whole genome shotgun sequence.
ACCESSION   DAORLS010000378 DAORLS010000000
VERSION     DAORLS010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20457_length_2705_cov_1.767925"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    286..363
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<364..1741)
                     /locus_tag="PK977_18560"
     CDS             complement(<364..1741)
                     /locus_tag="PK977_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20190.1"
                     /translation="MMPNRTTDILFQLIKSLEKAEKRHFKLYIKRSSGNADLKIIRLF
                     DVLDKMKDYDEAGLIKKMGDVTRPQLANLKAHLYKQLLASLRLLKSSDSLDLQLNEQF
                     DYAHILYKKGLFLQSLRILDRAKEIAKANQKFNFLPQIISLEKRIEGLHITRSMQNRA
                     EQLSVEANEVSRHIDNVARLSNLALKLYSWYIQHGHARNEEDEKDIKAFMREQLPKDA
                     WQQTGFYERLYLYQGFCWYAFIRQDFLQYYRYTQKWVDLFEEQPLMIRVETGHYIKGL
                     HNLLNAHFDLRNYKGFEKTLRRFEKVSQTDRVKEHDNFRIQAFIYINIARINQHFMMG
                     TFKEGLKLVPEIEAKMEEYHLFVDQHRILVLNYKIAMLYFGSGDYNTCIDYLQKIIHE
                     KTDLRYDLQCYARVVHLLAHYELGNDMLMESLSKSVYRFMAKMANLTVVEEAMFKFLR
                     HSFPLSPRQ"
     gene            2200..>2379
                     /locus_tag="PK977_18565"
     CDS             2200..>2379
                     /locus_tag="PK977_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRF20191.1"
                     /translation="MAVRIFITGGTFDKEYNEITGQLYFKDTHMHDLLELGRNKVPVE
                     IRTLMMVDSLEMTDED"
     assembly_gap    2380..2416
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2417..2690
                     /locus_tag="PK977_18570"
     CDS             <2417..2690
                     /locus_tag="PK977_18570"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRF20192.1"
                     /translation="EDRIIITHGTDTMSETAQYLAQKVKNKTIVLTGAMIPIKFGSSD
                     GLFNLGSALAFAQTLPVGVYVAMNGRYFNWDNVRKNKQTGMFEEVK"
BASE COUNT          754 a          528 c          541 g          767 t
ORIGIN      
        1 atgcaatgcc ataggctact tctgggtctg tgggtgcaaa aacatgggga acaccaaaac
       61 tatcccgcgc aattgtgata ttagccggat tgatctgtgc tttgctttgt aaagctgcta
      121 gcaacaggat gaacagaatt tttctcattg aggaaaatta aagaaaacat gctgttccgg
      181 gaaagacatt tatttatgcc tgctctgcaa atatttttca tgtatgatta cactcatcgg
      241 cttgttatac accttgcttt ccacccagga gataatatcg aggtannnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnngttgccg tggcgacaaa ggaaatgaat ggcgaaggaa tttaaacatg gcctcttcta
      421 ccacggtcag gttagccatc ttcgccataa accgataaac agatttgctc aatgattcca
      481 tcagcatatc attgcccaat tcataatgtg ccagcaggtg tactaccctt gcataacatt
      541 gcaggtcata acgaagatct gttttttcat gaatgatctt ttgcaggtaa tcgatgcagg
      601 tattataatc tccgctgcca aaatacagca tggctatttt gtagttcaac actaatatgc
      661 ggtgctggtc tacaaagaga tgatactcct ccatcttggc ctctatctcg ggtaccagct
      721 tcagtccttc cttgaaagtt cccatcatga aatgctggtt gatccgtgca atattgatgt
      781 agatgaaggc ctggatacga aagttatcat gctctttcac cctgtctgtc tgcgaaactt
      841 tctcaaaacg acgaagtgtt ttttcaaacc ctttgtaatt acgtaaatca aaatgggcat
      901 tcaaaagatt gtgcaatcct ttaatgtaat gaccggtttc taccctaatc attaatggtt
      961 gttcctcaaa aaggtcaacc catttttgag tgtaacggta atattgtaaa aaatcctggc
     1021 gaataaatgc ataccagcaa aaaccctggt acaggtagag ccgttcataa aaaccggtct
     1081 gctgccaggc atctttgggt aactgctctc tcataaatgc cttgatatcc ttttcatctt
     1141 cttcattgcg ggcatgtcca tgctgaatat accagctgta taatttcagc gccaggttgg
     1201 agagccgggc cacattatcg atatgccggc ttacttcgtt ggcttcaaca gagagttgct
     1261 ctgcccggtt ctgcatactg cgggtaatat gcaacccttc gattctcttt tctaatgaaa
     1321 tgatctgggg aagaaaattg aacttctggt tggcctttgc tatttccttg gcacggtcaa
     1381 gtatacgaag gctttgaagg aacaacccct ttttataaag gatatgagca tagtcgaatt
     1441 gttcattgag ttgaagatcg agactgtctg aactttttaa cagccggaga gaagccagca
     1501 gctgtttata caagtgtgct ttcaggtttg ccagttgcgg ccgggtcaca tcacccatct
     1561 tctttatcaa tccggcttcg tcgtagtctt tcattttgtc cagcacatca aatagccgga
     1621 tgatcttaag gtctgcatta cccgaactgc gctttatata cagtttaaaa tgcctttttt
     1681 cagccttttc aagcgatttt atcaattgaa ataatatgtc ggtagtgcgg ttgggcatca
     1741 tacgtctttt ttgctgaaat cagccaccag taagggtttc accaatcgtc ctatcctttt
     1801 ggcataatga aatgatgccg tatttggttt gaaggacaag ggagggcttc ctacttttgg
     1861 tgtagaagat taatcataca gcaagacaat cgtcagaggg gcaagcgtca gaggtcaaag
     1921 ttaaagctaa aaatcttcta aagagctggt aaaacagcca acaacccgca cttcgaaaat
     1981 tttcatatgg caagcaatcg ttagaggtca gtccgtttct acggaaggac cttttttata
     2041 taaaggcctg ctaaagtaac agtttcttaa tattagaaaa gcgaaatcac tgtttttttt
     2101 attttttcta ttgacttaaa taacctgttg aataacgaca attggtcaga gagctgcgaa
     2161 aaaaagtcaa ttaatatctt tataccctaa accatgacta tggcagtacg cattttcatc
     2221 accggcggca ctttcgataa agaatataat gaaataacgg gacagctgta ttttaaagac
     2281 acccatatgc atgatctgct ggagctgggt cgtaataagg ttcctgtcga aatacgaaca
     2341 ttgatgatgg tagacagcct ggaaatgact gatgaagacn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnagaa gacaggatca tcattacaca tggcacagac accatgagcg
     2461 aaacggcaca atacctggca caaaaagtaa aaaacaaaac tattgtactt actggtgcca
     2521 tgatccccat taagtttggc agtagcgatg gtttattcaa cctgggaagt gcattggctt
     2581 ttgcacaaac acttccggtt ggtgtgtatg tggctatgaa tggtcggtat tttaactggg
     2641 ataacgtccg aaagaacaag cagacgggta tgtttgaaga agtgaaataa cttaaacttc
     2701 tatat
//
LOCUS       DAORLS010000379         2700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20508_length_2700_cov_2.258223, whole genome shotgun sequence.
ACCESSION   DAORLS010000379 DAORLS010000000
VERSION     DAORLS010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20508_length_2700_cov_2.258223"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..737)
                     /locus_tag="PK977_18575"
     CDS             complement(237..737)
                     /locus_tag="PK977_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20193.1"
                     /translation="MKTNVLFIAVLLLLSCNHQQSPQQTDSKETLLNTAWSVKGCAEK
                     ATRSNTKFPDSGEYNDYPDLPPPEGLGIKAGGDSITYSRFEQHLCCRQVKVSVEKNEN
                     IITFTEYWFGQGCKCRCSSTVHAVIRELPKGEYQVYGVATGTDPVENKPTGIKDTVLA
                     KKVTIQ"
     gene            complement(748..>2112)
                     /locus_tag="PK977_18580"
     CDS             complement(748..>2112)
                     /locus_tag="PK977_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20194.1"
                     /translation="AENVTYHKVRKTGDTIVAPVIQLSRTNRVNLQLVNAANSYQRRI
                     QYVEKEETPETVIIPGMGSDPSKNLVSGLADIDLSDLKSAEIFKRGGGNKDVEALFES
                     SQQKAAKQEFTNQYNAFTKAYAQWQKAMLYEQNCRVLWKDLAQLRYSMQYHAAEVKKT
                     AKSKTETIFPGAAENPSAILLNNNAANLKTSVASVKNSLTSLQAVYDSFRELEIKSPI
                     ADSLMENIKDEAALVNRKYDTGKEPDEVIDRIAELFRQILNDSYTQLTPLDVTILAEI
                     SFIPVIDSVTAFTLNINPADTIRRLVPIYKKEPLRFRNTFGFGFASFAESRWNYYVTP
                     DSVIAKESANQFKPLVVTYLHFYAPRDKGFRWGGTFGAGLPLSGNKDINIMIGLSTFL
                     GKNDPVCITAGIAGSELKKLTGIKPGDKVSFTEIDDKHYQSVYRIGYFVSLTFNPGSL
                     NTKD"
     assembly_gap    2113..2263
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2449..>2700
                     /locus_tag="PK977_18585"
     CDS             2449..>2700
                     /locus_tag="PK977_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20195.1"
                     /translation="MSYPSLITNKNTLWSGKWLLPVILLGIITLLSSPLSAQTALSQL
                     ENWAGARISSVNVPAPTAPSGGNYTYRLPFSKTPEQKSAE"
BASE COUNT          675 a          575 c          497 g          802 t
ORIGIN      
        1 taattttctc cgtgcactca gtggttaatc ggatgtttcg tatttttgca ttcccgaaac
       61 tcacccccaa cccctctctc cctgaaaata cagattcact aagcatacaa atatagcggt
      121 tttaaataat caaccatttc cttctgttac cctgaatttt gtgaagggca atcatggccg
      181 caaattacat gtaatgaatg agagcctcaa acaagggggc tgctgtgaat aagtatttac
      241 tgaatggtta cttttttagc cagcaccgta tccttaatac cagttggttt attttcaacc
      301 ggatccgtac ctgtagcaac accatatacc tggtactcac cttttggtaa ctcacggatt
      361 actgcatgca cagttgaact gcatcggcat ttacagcctt gcccaaacca atactcagta
      421 aaagtgatga tgttttcgtt tttctcaact gataccttaa cctgcctgca gcaaagatgt
      481 tgttcaaacc ggctgtaagt aatagaatct cccccggcct taatacctaa gccttctggt
      541 ggcggcaggt caggataatc attgtactca ccggagtcag gaaatttcgt attactgcgg
      601 gtagcttttt ctgcacagcc ttttacactc catgcagtat tcagcaaagt ttctttgcta
      661 tctgtttgct gtggcgactg ctggtgatta caggaaagaa gtaacaggac ggcaatgaac
      721 aggacgtttg ttttcatttt agtcagttta atctttagtg tttaagctac cagggttaaa
      781 tgtgagagaa acaaaataac caatccggta aacagactgg tagtgcttat catcgatttc
      841 tgtgaagctc actttatctc ccggctttat cccggtaagt ttcttaagtt cagacccggc
      901 gatgccggca gtaatacaaa caggatcatt cttgcctaaa aacgtactca ggccgatcat
      961 gatattgata tccttgtttc cgcttagtgg aagaccagct ccaaaagtgc cgccccagcg
     1021 gaacccttta tctcttggtg cataaaaatg gagataagtt accaccaacg gtttaaattg
     1081 gttggccgat tcttttgcaa ttacactatc cggggtaaca taataattcc accggctttc
     1141 agcaaatgat gcaaaaccga aaccgaatgt atttctgaac cgtagcggct cttttttata
     1201 gatcggtact aatcgtctta ttgtatccgc agggtttata ttgagtgtaa aagccgtaac
     1261 tgaatcgatt acgggaataa aactgatctc tgccaaaatg gttacatcta aaggcgtcaa
     1321 ctgcgtatag ctgtcattca atatttgcct gaatagttcg gcaatcctgt ctatcacttc
     1381 atccggctcc ttccctgtat catatttcct gtttaccagc gccgcctcat cctttatgtt
     1441 ttccatcaaa gaatctgcga taggagattt tatttccagt tccctgaaag agtcataaac
     1501 cgcctgaagg gatgtcaagc tgttttttac agatgcgacc gaggttttaa ggttggccgc
     1561 attattattc agcaaaatag cagatggatt ttcggctgca ccaggaaaaa ttgtttccgt
     1621 tttactttta gcagttttct ttacttcggc agcgtggtat tgcatgctgt agcgaagctg
     1681 tgcaaggtct ttccacaaca ccctgcaatt ttgttcatac aacatagctt tttgccattg
     1741 tgcataggct ttggtaaagg cattgtattg gttggtaaac tcttgtttgg ctgctttttg
     1801 ctggctgctt tcaaataaag cctctacatc tttattcccc ccgcctcttt taaaaatttc
     1861 tgccgatttc aaatcgctca ggtctatatc cgccaggcca cttaccaggt ttttagaagg
     1921 atctgatccc atacccggaa tgattactgt ctcaggtgtt tcttcctttt ctacgtactg
     1981 aatccggcgc tgatagctgt tggctgcatt taccagttgc aggtttaccc ggttagtccg
     2041 ggagagttgt ataaccggcg caacaatagt atcgccggtt tttctcactt tgtgataagt
     2101 aacgttttca gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagcagt aatgacctgt
     2281 atcattaact ggtttgcttt aactcataat aatccaccgt agtacatact atctttcagc
     2341 acaaataaac ctaggggact gtttcgtctg atatattcag atgaattgtt aatggtattg
     2401 ttattctttt ctgaaaagca atttgcagaa tgtcagaaaa tcttcatcat gtcttatccg
     2461 tctttgataa caaataagaa taccctgtgg agtggaaaat ggcttttacc agttattctt
     2521 ttaggtataa taacattact ttccagcccc ttgtcagcac aaactgccct gagccagctt
     2581 gaaaactggg ccggagccag gatatcatca gtaaatgttc ctgcccctac ggctcccagc
     2641 ggagggaatt acacttaccg attacctttt tcaaaaacac cggaacaaaa atctgccgaa
//
LOCUS       DAORLS010000380         2695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20582_length_2695_cov_2.010606, whole genome shotgun sequence.
ACCESSION   DAORLS010000380 DAORLS010000000
VERSION     DAORLS010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20582_length_2695_cov_2.010606"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    257..306
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<307..917)
                     /locus_tag="PK977_18590"
     CDS             complement(<307..917)
                     /locus_tag="PK977_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20196.1"
                     /translation="MRKFIRIVLCLVVPFSVRAQTLQNPLLSPYTGFGVYSSGHTDVF
                     SFTNNQASLAGIKNFSAGLFGERRFMLSELNNYLAVAALPTGSGNFGLKASYAGFTDY
                     NETQIGLAYARSLGKKVDIGAQFNYNGIRISGYGEASAVSFELGTILHITDKLHAGVQ
                     VNNPVGGKFGKDQEKLPSVYSFGLGYEASEKLLLSAEIEKEEN"
     gene            983..1894
                     /locus_tag="PK977_18595"
     CDS             983..1894
                     /locus_tag="PK977_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRF20197.1"
                     /translation="MRWIAQLPLLPLRHANFRREKSSTIKDELKIDVAQLAAEGKKIP
                     HLAAILVGNNGASETYVGAKVKACEEVGFRSTLVRLDENISENKLLDEIRLLNTDPDV
                     DGILVQLPLPKHISDEEVINAIDPDKDVDGFHPVNVGYMLQGLPTFVPATPHGIMLLL
                     QHYKIETAGKHAVVVGRSNIVGRPMSVLLSSNTNPGNCTVTVCHSKTNNIKEFCLQAD
                     IIVAALGKPEFVTADMVKEGAVVIDVGITRMEDPTKKSGFKLKGDVEFDTVAPKCSWI
                     TPVPGGVGPMTIAALMKNTYKSCMQKN"
     gene            1895..2524
                     /locus_tag="PK977_18600"
     CDS             1895..2524
                     /locus_tag="PK977_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRF20198.1"
                     /translation="MAETITNNISTATLPVMEHFYTIQGEGYHQGRAAYFIRLGGCDV
                     GCVWCDVKESWDASKHPLLKIEDLVTAVKKTPAELVVITGGEPLMHNLDELTDQLQEA
                     GFKTNIETSGSHPLSGLWDWICLSPKKFKAPLEPILHVANELKVIIYHKSDFEWAEKY
                     AALVSPHCKLYLQPEWDKAPEMTSAIINYILDNPQWELSLQIHKYINVP"
BASE COUNT          816 a          565 c          563 g          701 t
ORIGIN      
        1 gctgttctgt tgccggggga atttcctgtc cataaagaac agaacagcca tgcgaaaaaa
       61 caattatcaa tatgataata taatttttca cttctgctgt ttctttagat caaacactaa
      121 catcagcccc ggtgtaatac ccaactgcgg atggtaatta ctggtaatat caattcgcac
      181 tgcatttaaa cgtatgccga caccggccca aacgattgat gcagcagaag acatcccggc
      241 cctcacctgc agctgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnntgat tttcttcttt ctctatttct gcactgagca gcaatttttc ggaagcttca
      361 taccccaaac caaatgaata aacagagggg agcttctcct ggtcttttcc aaattttcct
      421 ccaacaggat tattaacctg tacacctgca tgcagtttat cagtgatgtg caaaatagtt
      481 cccagttcaa aactaaccgc cgatgcctca ccatagccgc ttattcgaat accgttgtaa
      541 ttaaactgag cacctatatc cacttttttt ccaaggctgc gggcataggc aagcccaatt
      601 tgtgtttcat tgtaatcagt aaagccagca taggaagcct ttaatccaaa gtttcccgaa
      661 cccgtcggca atgccgccac agccaaataa ttgtttagtt cgctgagcat aaaccgtctt
      721 tctccaaata gtccggctga aaaatttttt atcccggcca gtgaagcctg gttattcgtg
      781 aaagaaaaaa catcggtgtg accggagcta taaacgccaa aaccggtgta aggtgagagg
      841 agcggatttt gcaatgtttg tgccctgact gaaaagggca caactagaca taagacgatt
      901 ctgatgaatt ttctcaaagc ggataatgtt tataggagaa agttacaatc cggttttttt
      961 tctgcaaaat aaaaagatac cattgcgatg gatagcacag ttgccattgc tacctttgcg
     1021 tcatgcaaat tttagacggg aaaaaagcag caccattaaa gatgagctga aaatagatgt
     1081 agcccaactg gctgcagaag ggaaaaaaat accacacctg gcagcaatac tcgttggcaa
     1141 caacggcgcc agtgaaactt atgtaggcgc caaagtaaaa gcctgtgaag aagtaggatt
     1201 ccggtctaca ttggtacgac tggatgaaaa tatatcggaa aacaaattac tggatgagat
     1261 acggttgctc aatacagacc ctgatgtgga tggcatccta gtacaattac cattaccaaa
     1321 acatataagc gatgaagaag tgatcaatgc cattgatccg gataaggatg tggatggttt
     1381 tcacccggtg aatgtgggct atatgctgca gggccttccc acttttgttc cggctacacc
     1441 gcatggtatt atgttgctgc ttcagcatta taaaattgaa actgccggta aacatgccgt
     1501 tgtcgttggg cgcagcaata ttgtcggcag acccatgagt gttttattaa gcagcaatac
     1561 caatccgggc aactgcacgg ttactgtatg ccattccaaa acaaataaca taaaagagtt
     1621 ttgtttacag gccgatatta ttgttgctgc actcggtaaa cctgaatttg taaccgcaga
     1681 tatggtaaaa gaaggcgcag tagttattga tgtaggcata acaagaatgg aagacccgac
     1741 aaaaaaaagc ggcttcaagc tgaaaggcga tgtggaattt gatactgttg cgcctaaatg
     1801 ttcctggata acaccagtac ccggtggtgt tgggccaatg accattgctg cattgatgaa
     1861 gaacacatat aaatcctgca tgcagaaaaa ctaaatggca gaaacaatca caaataatat
     1921 cagtacggcg actcttccgg taatggaaca tttctatacc atacagggag aaggctatca
     1981 ccaggggaga gcagcttatt ttatccggct ggggggttgt gatgtgggtt gtgtgtggtg
     2041 tgatgtaaaa gaaagctggg atgcttccaa acatccctta ctaaaaattg aagacctggt
     2101 aactgccgta aaaaaaacac cggctgaatt agtggtcatc accgggggtg aacctttgat
     2161 gcataacctc gatgaactaa cagatcaatt acaggaggcc ggttttaaaa caaatattga
     2221 aacatccggc tctcatccgc taagcggctt gtgggactgg atctgtcttt ctcctaaaaa
     2281 attcaaagcg ccgctggagc cgatactgca tgtggccaat gaactaaaag taatcatcta
     2341 ccataaatca gattttgaat gggcggagaa atatgctgcc cttgtttcac cgcattgtaa
     2401 gctttacctg cagccagagt gggacaaagc cccagaaatg acgtctgcca tcatcaacta
     2461 tattttggat aacccgcaat gggaactttc actgcaaatc cataagtaca tcaatgtacc
     2521 ttaagcaacg ttttgtttaa caatttcttc cagtgaaagg aataaattct ttttagcttc
     2581 gttattcgct aatacaaatc cattcgatga agaaactgtt gcttttcatt ttactttttt
     2641 cagctataac ggctttggcg caaccttatg acccaacaaa agttaataag aaagc
//
LOCUS       DAORLS010000381         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20682_length_2683_cov_2.220320, whole genome shotgun sequence.
ACCESSION   DAORLS010000381 DAORLS010000000
VERSION     DAORLS010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20682_length_2683_cov_2.220320"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..837
                     /locus_tag="PK977_18605"
     CDS             7..837
                     /locus_tag="PK977_18605"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF20199.1"
                     /translation="MPGNDSAEIAEKKGSVTTGVGTALSKYGKANQLYVSMVDSLSRV
                     LFRYPLIDSSGLNYAKEWVGTPNMGFRRPNYVIIHHTANNSCEATLQEFTTPGGREAS
                     AHYVICKDGTVHHMLNDLLRSHHAGDSKWGNNTDINSSSIGIELVNNGSQPYTEEQLN
                     SLAILLDRLKTAYKIPAPNFIGHGDVAPTRKADPSSKFPWKEMSEKGFGLWWDDTTGV
                     EVPENFDYLLGLRIIGYDISAPASVVAAFKRHFMKDGSGGSMNAAVRKIIYVLYRKYL
                     "
     gene            921..1730
                     /locus_tag="PK977_18610"
     CDS             921..1730
                     /locus_tag="PK977_18610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF20200.1"
                     /translation="MRKLQALILAITLLFTYSCNTGKYAATNKSYKKQVKKFAKALAE
                     YPVKDSFTTAEEWVGTTNFSMRKPNYVIIHHTAQDSCGQTLRTFTLPRTQVSSHYVIC
                     KDGTVHHMLNDLLRGHHAGVSKWGNATDLNSSSVGIEIDNNGYEIFSDEQINSLLIVL
                     DRLKKAYNIPTANFIGHADIAPGRKVDPNRNFPWQKLAQNGFGHWYDTTNTIVPVDFN
                     PIQALRIIGYDIKNEKNAIQSYKIHFVQKDTTKTITETDKKILFELMKKYQ"
     gene            complement(1724..>2683)
                     /locus_tag="PK977_18615"
     CDS             complement(1724..>2683)
                     /locus_tag="PK977_18615"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF20201.1"
                     /translation="DGSYQSNSVDVLTNLPVISLYGDHLAPTAEELNDIDIVLFDIPD
                     VGCRFYTYLWTMTYVMEACAANDKSIIILDRPNPIGGDLEKAEGPGLDETFCSSFIGR
                     WNIPVRHSCTLGELATYFAGTKINNCDLQIIKVKKWNRLHTASEAAWHFLPTSPAISD
                     AQTALLYPGMGLLEGVNVNEGRGTDYPFKIFGAPWIETSLLYEKFAQSNPAGITIKKI
                     SYTPETGIYTGEICHGLKIAVSDTPSFKPVETGLLLLQLIARLFSGKCEERLYPTVAN
                     PLGCGHLDKLTGIHHSFGKIKTGSILTTDVSDWKEIIRPFLLY"
BASE COUNT          804 a          542 c          576 g          761 t
ORIGIN      
        1 tccgcagtgc ccggaaatga ttcagctgaa attgctgaaa agaagggatc ggtaacaaca
       61 ggagtcggta cagctttatc gaaatatgga aaggccaacc agttgtatgt aagtatggtt
      121 gactcacttt ccagggtgct tttcaggtat cctcttattg attcttccgg ccttaattat
      181 gcaaaagaat gggtaggtac acccaatatg ggttttcgtc gccccaatta tgtgatcata
      241 catcatacgg cgaataacag ttgcgaagca accttacagg aattcaccac cccgggcggt
      301 agagaggcca gtgctcatta tgttatttgt aaagatggaa cggtacacca tatgctcaat
      361 gatctgttgc gttcacatca tgccggcgac agcaaatggg gtaataatac cgacatcaat
      421 tcaagcagta ttggaattga attggttaat aatgggtctc agccctatac ggaagaacaa
      481 ttaaacagtc ttgcgatatt actcgaccgt ttgaaaacag cctataaaat acctgctccc
      541 aattttatcg gtcatggaga tgtggcacct acgagaaaag ctgatcccag ttcaaagttt
      601 ccctggaagg aaatgagcga aaaaggattt ggtctttggt gggatgatac aaccggggta
      661 gaagtacccg aaaattttga ttacctgttg gggttacgca tcatcggcta tgatatttca
      721 gctcctgcct ctgtagtggc agcgttcaaa aggcacttta tgaaagatgg atcaggaggg
      781 tcaatgaatg cggcagtccg taaaatcatt tatgttctct ataggaaata cctgtaattg
      841 acaattctgt cagcattctc tctctattaa gtagaccggc cgggctttct ttcatacatt
      901 agcattctca atctgatagt atgcgaaagt tacaggcact gattttagca attactctgc
      961 tgtttactta tagctgcaac accggcaaat atgcagccac taataaaagc tataaaaagc
     1021 aggtaaaaaa gtttgccaaa gcactggcag aatacccggt aaaagattct tttactacgg
     1081 cagaagaatg ggtaggtact accaacttct ctatgcggaa acctaattat gtaatcattc
     1141 atcatacagc acaggacagt tgcggacaga ccttaagaac ctttacgctg ccaaggacac
     1201 aagtaagttc ccattatgtt atttgtaaag acgggactgt acaccatatg ctgaatgatt
     1261 tgctgagagg gcatcatgca ggagtaagca agtggggtaa tgccaccgat cttaattcat
     1321 ccagtgtagg aatcgagatt gataataacg ggtatgagat attttcagat gagcagatca
     1381 acagcctttt aattgtattg gacaggctca agaaagctta taatatccca acagccaact
     1441 ttattggcca tgccgacatt gcccctggaa gaaaagtaga tcctaaccgg aattttccct
     1501 ggcaaaagct tgctcagaat ggctttggcc attggtatga tactacaaat acaatagtac
     1561 ctgttgattt taatcccatc caggcattac gaattatagg gtatgacata aagaatgaga
     1621 agaacgctat tcaatcttat aaaatccatt tcgttcaaaa ggatacaacg aagaccatta
     1681 ctgaaacaga taagaagata ctttttgagc tgatgaagaa gtatcagtaa agaaggaaag
     1741 gccggattat ttctttccag tcgctgacat cagttgttaa aatgctacca gttttaatct
     1801 ttccaaatga atggtgtatg cctgtcagtt tatcaagatg accacagcct aaaggatttg
     1861 ccacagtcgg gtataatctt tcctcacatt tgccggagaa tagccgggca attagttgta
     1921 ataatagtaa gccagtttca actggtttga aagaaggagt atctgatact gcaattttta
     1981 atccgtgaca gatttctccg gtatagattc ctgtttcggg ggtataagaa attttcttta
     2041 ttgtaattcc tgcagggttt gattgagcga atttctcgta caaaagggag gtttctatcc
     2101 agggtgctcc gaaaatctta aatggataat cggtgcctcg tccctcgttc acattgactc
     2161 cctccagtaa ccccatacca ggatataaga gagctgtttg cgcatcactg atggcaggag
     2221 aagttggcag aaaatgccat gctgcttctg acgcagtgtg caacctgttc cattttttaa
     2281 ctttaattat ttgcaggtcg cagttattga tttttgtgcc ggcaaagtaa gttgccagtt
     2341 ctcctaaagt gcaactatgc cttacaggta tattccatct gccgataaaa gaactgcaaa
     2401 atgtttcatc cagcccggga ccttctgctt tttctaaatc tcctccgatt ggatttggtc
     2461 tgtcgagaat aataatagat ttgtcgttgg ctgcacaggc ttccatcaca taggtcattg
     2521 tccacagata agtatagaaa cggcagccca catccggaat atcaaatagc actatgtcaa
     2581 tatcgttcag ttcttctgcc gttggtgcca ggtgatcacc ataaaggctg atcaccggta
     2641 aatttgtcaa gacgtcaaca ctattgcttt gataagaacc atc
//
LOCUS       DAORLS010000382         2677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20751_length_2677_cov_1.840580, whole genome shotgun sequence.
ACCESSION   DAORLS010000382 DAORLS010000000
VERSION     DAORLS010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20751_length_2677_cov_1.840580"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PK977_18620"
     CDS             <1..1375
                     /locus_tag="PK977_18620"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRF20202.1"
                     /translation="ATRPGRFMIPGTTIVADGQLLQSDDVWVKVITKEEATLLFGREM
                     AAMNSDYILRPGEDPYQKIRKNLFLKVLVDKRSCYAGEPVTATFKLYSRLESKSDIVK
                     NPGFYGFTVFDMVNLADKQVAAEMLNGKVYDVHTIRKVQLYPLQAGVFTIDPMEVRNK
                     VEFSRSVVNKKTEQQIAEGMLSGSEEEPANKHAEVFESSMSSEAVTITVRPSPEKNKP
                     ALFTGATGNFSISAALVRTELRKNEEAWLEVTVSGKGNFLQLIPPAVQWPAAVETFDA
                     ANTDKLDKTKVPLAGSRTFRFPFVAAEEGDFEIPAISYAFFDPDSNRYKTMTTKPVRF
                     RVSGETVKEKVVTEKKKSVSSINTRASWVAGSIVAALFLAVFGYWLLSKKEPVVVKAE
                     ETLVVEPSADEQLKSATQLAQHNDPAFYNELHRVIWEKLGHYFGLEGTNNQIQTHPID
                     WGVFKTG"
     gene            1395..1520
                     /locus_tag="PK977_18625"
     CDS             1395..1520
                     /locus_tag="PK977_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20203.1"
                     /translation="MTNTINHILTECETNLYTTAALESNKEQLLADARKVLASLA"
     gene            complement(1524..2231)
                     /locus_tag="PK977_18630"
     CDS             complement(1524..2231)
                     /locus_tag="PK977_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF20204.1"
                     /translation="MSTDRKTSILLVEDEENLHEALKLNLELEGYDVTSAFDGAAALK
                     AIQSEYFDLIILDVMLPEVDGINVTETVRLTNNEVPILILSAKNTSADRVLGLKKGAD
                     DYLTKPFNLEELLLRVNKLINKNKKLQDKSTIGDTYSFGGNNIDFKAQEATKSNGEKI
                     QLSKKESMLLKLLIENKNEVVPREKILQSVWGYNVYPTTRTIDNFILNFRKYFEEDSR
                     NPKYFHSVRGVGYKYAE"
     assembly_gap    2383..2432
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2433..>2677)
                     /locus_tag="PK977_18635"
     CDS             complement(<2433..>2677)
                     /locus_tag="PK977_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HRF20205.1"
                     /translation="ELNLSDLLKDCLQDFRNRFPDRIFLEEIELDAEVLGDPLLLQML
                     INNLLENAVKYAPKEKPVKAVLKKEAGAVTLQIIDEG"
BASE COUNT          726 a          554 c          615 g          732 t
ORIGIN      
        1 agctacccgg ccgggccggt ttatgattcc cggtactacc attgttgctg acggacaatt
       61 actgcaaagc gatgatgtgt gggtgaaagt gattacaaaa gaagaagcca cgttgttgtt
      121 tggccgggaa atggcggcca tgaattctga ttatatcctt cgcccgggtg aagacccgta
      181 tcaaaagatc aggaagaatc ttttcctgaa agtactggtg gataaacgca gttgctatgc
      241 cggtgagccg gttacagcca ccttcaaatt atattcaagg ctggaatcaa aatcagatat
      301 tgttaaaaat cccggtttct atggctttac tgtttttgat atggtgaacc tcgcagacaa
      361 acaggtagca gctgaaatgc taaatggaaa agtatacgat gtccacacca tccgcaaagt
      421 gcagctttat cctttgcagg caggcgtttt cactattgat ccgatggagg taaggaataa
      481 agtagagttt tcccgcagtg tggtgaataa aaaaacggaa caacaaatag ccgaaggtat
      541 gttgagcggc agtgaggaag agcctgcgaa taagcatgct gaggtatttg aaagcagcat
      601 gtcaagtgaa gccgtaacga tcactgtcag gccttcgccg gagaaaaata aaccagccct
      661 cttcaccggc gcaacaggca atttttctat cagtgctgcg ctggtcagaa ccgagctgag
      721 aaaaaatgaa gaagcctggc tggaagttac tgtatccggt aaagggaatt tcttgcaact
      781 gataccgcca gctgttcaat ggcctgccgc tgttgaaaca ttcgatgcag ctaatacgga
      841 taaactggat aagacgaaag tgccgcttgc gggaagcaga acctttcgtt ttccttttgt
      901 agctgccgag gaaggcgatt ttgaaattcc ggctatttcg tatgcttttt ttgatcccga
      961 tagtaaccgg tacaaaacga tgactaccaa accagttcgg ttccgtgtaa gtggtgaaac
     1021 ggtgaaagaa aaagtggtga cagaaaagaa aaagtctgtc agcagcatta atacaagggc
     1081 cagttgggtg gcgggcagca tagtagcagc acttttcctg gcagtatttg gttattggtt
     1141 actcagcaaa aaggaaccgg tggtggtaaa agcggaagaa acattggtag ttgaaccatc
     1201 agctgatgag cagttaaaat cggctacgca actggcgcag cataatgatc cggcattcta
     1261 taatgagttg caccgtgtta tttgggaaaa gctggggcat tattttggcc tggagggaac
     1321 aaacaaccag attcaaacac acccaattga ttggggtgta ttcaaaactg gttgaaaaag
     1381 aaatcagccc ggaaatgacg aatactataa atcacatttt gacagaatgt gagaccaatc
     1441 tttatacaac cgcagcattg gagagtaata aggaacaact gctggcagat gcccgtaaag
     1501 tgctggcatc attggcatga tgcttattca gcgtatttat aacctacgcc tcttaccgaa
     1561 tggaagtatt tagggttgcg gctgtcttct tcaaagtatt tgcggaagtt caggatgaaa
     1621 ttatcgatgg tccttgtggt gggatacaca ttatagcccc agactgactg caggatcttt
     1681 tccctcggca ctacttcatt tttattttca ataagcagtt tcagcagcat ggattctttt
     1741 ttgcttaact gaattttttc accgttgctt ttagttgctt cctgtgcttt gaagtcaata
     1801 ttgtttccgc caaaagaata cgtatcgcca atagttgatt tatcctgtag cttcttgttc
     1861 ttgttgatca atttatttac ccttaacaaa agctcttcga ggttgaaggg cttggtcagg
     1921 taatcatcgg cgcccttttt caatccaagc accctgtcgg cactggtatt tttggcactt
     1981 agtatcagaa tcggcacttc attattggtg aggcgtactg tttcagtcac attaatccca
     2041 tccacttcag gcaacatcac atccagtata ataaggtcaa aatattctga ctggattgct
     2101 ttgagggcag cagcaccatc aaaggcagaa gtaacatcat agccttccag ctcaagattc
     2161 aacttcagcg cttcgtgcag gttttcttca tcttcaacca gcaggataga tgttttccgg
     2221 tctgtactca tgttttaaaa atgactgcaa aaatactgcc ctgtggcaaa tggtttgtca
     2281 ccgaaatgtc ggcattgtgg tcctcagcta tcttgcggca aaggtacaat ccgagtcctg
     2341 tgccctgtgt ttttcgggtg gcttcgttgc caatgcggta aannnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccttcat caataatctg aagcgtcact
     2461 gcgccggctt cttttttcag tacagctttt accggttttt ccttcggcgc atactttact
     2521 gcgttttcca acaggttatt gatcagcatc tgtaacaata aagggtcacc caatacttca
     2581 gcatccaatt ctatttcctc caggaatatc cggtcgggaa accggtttcg gaaatcctgc
     2641 aggcagtctt tcagcaagtc ggacagatta agctcat
//
LOCUS       DAORLS010000383         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20756_length_2676_cov_2.117512, whole genome shotgun sequence.
ACCESSION   DAORLS010000383 DAORLS010000000
VERSION     DAORLS010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20756_length_2676_cov_2.117512"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PK977_18640"
     CDS             complement(<1..1599)
                     /locus_tag="PK977_18640"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HRF20206.1"
                     /translation="MRTTRLMLLVCFSVCAVEALGQNLNNPNKQGPLGTQVNTLSGNL
                     YIPRTDFYVPARGFDLNIRFNYNSFLFTEEYGYGKGWTFEYNIRYKYDTIPGSRLILW
                     GDGREDQYDSLPGGGYKTPRGFFNTLSQYQPNKYVLTELNGVKYFFDNAVHKRITRME
                     EPNGNYINFIYSDSLLVTMTNAAGQSISFNYDPQGRLISMTDAIAVPSRTFIYTYDTG
                     GNLKEVTDPLGNKYRYTYLINGPMKSLADKNNNKVDIIYFPDLTVRELIGCNKRLSFS
                     YDTVSKTTFVTDHLSTGNQVTKYKYETVDNLSWITSMSGNCCGFNVSYEYDDQGNLLK
                     MTDANGQVYTYTYDEFGNPLTATDPLNQTSTYTYTTDFKKISSFKDPKGNLYTISYDS
                     RGNMTQLVTPGNNIYTATYNTNGDIVSSTDPKGNTYTYNYDSYGNPVNVTGPNSYQAV
                     LSFDARGNLLSYTDARGNTSSAEYDILDRLKKITDPINYNVQFTYDAQGNPVSFKNKN
                     NETTILNFDASNRLVNITGPTGNQS"
     gene            complement(1653..>2306)
                     /locus_tag="PK977_18645"
     CDS             complement(1653..>2306)
                     /locus_tag="PK977_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20207.1"
                     /translation="GNIGNVTVRIRGSLFRDSMIAKLSNGTTTIYASAVYYTNNTQVF
                     ATFPLQGKPTGLYDLTLIKPDSSTAVLANGFSIVNANNGGLITGGGPNTGPGNGNAPG
                     CDPGAASGLNSQLVVELIVPEKVVRGRPVVIVINFTNPTNFDLPVQTRILYNDEDVKM
                     AFTKAGIPNGTSTLYIEFSEPDGPPGILRPGGGGSIIVHCIAPPQVPEDKSVLFKLQ"
     assembly_gap    2307..2439
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2440..>2676)
                     /locus_tag="PK977_18650"
     CDS             complement(<2440..>2676)
                     /locus_tag="PK977_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20208.1"
                     /translation="ELKLGIPELNTLQTVNRYYKLHIPDSLQGATILVTLKTNDSLLV
                     RNEMYIGGGFVPTPANYDYRFETPNYGNQQIVLS"
BASE COUNT          663 a          537 c          545 g          798 t
ORIGIN      
        1 tgattgattg ccggttggcc cggttatatt caccagcctg ttgctggcat caaaattcag
       61 gatagttgtt tcgttgtttt tgtttttgaa gcttaccggg ttcccctgtg catcgtaagt
      121 aaactgtaca ttgtaattga taggatcagt tatttttttc agcctgtcca ggatatcgta
      181 ttcggcggaa gaagtattgc ccctggcatc ggtataggac aaaaggttgc cccttgcatc
      241 aaaggacaga actgcctggt aactgttggg gccggtcaca tttacagggt tgccatagct
      301 gtcgtaatta taagtataag tattgccctt agggtctgta ctggatacaa tatcaccgtt
      361 tgtattatag gtggccgtgt aaatattatt acccggtgtt accagttgcg tcatattacc
      421 acgactgtca taactaatgg tgtaaaggtt ccctttaggg tctttaaagc tgcttatctt
      481 tttaaaatct gtggtataag tgtaagtgct tgtttgattt aaggggtcag tggccgtaag
      541 ggggttaccg aattcatcgt aagtatatgt atatacctgt ccgttggcat cggtcatttt
      601 aagcaggttg ccctggtcat catattcata actcacatta aagccacaac agttcccgga
      661 catggaagtg atccatgata aattgtcaac cgtttcgtat ttgtattttg ttacctggtt
      721 gccggtactg aggtggtcgg taacaaatgt agttttggaa acagtgtcat aagaaaaact
      781 caacctttta ttacagccta tcagttcccg tactgtcagg tcggggaagt aaataatgtc
      841 tactttatta ttgtttttat cggctagcga tttcatcggg ccgttaataa gataagtgta
      901 ccggtattta ttacccagcg ggtctgttac ttctttcagg ttaccacccg tatcatatgt
      961 ataaataaat gtccggctgg gcacagcaat tgcatctgtc atagatatca gtcttccctg
     1021 cgggtcataa ttaaaactaa tgctttgccc tgcagcattg gtcatggtga cgagcaggct
     1081 gtcgctgtat ataaaattga tgtagttacc atttggctcc tccatccggg tgattcgttt
     1141 gtgcaccgca ttatcaaaaa aatatttcac cccgtttaat tcggtcagta catatttatt
     1201 gggctggtat tgtgaaagcg tattaaagaa gccgcgggga gttttatacc ctccgccggg
     1261 taaggaatcg tattgatcct ctcttccgtc gccccataat atcagccttg aacccggtat
     1321 tgtatcatac ttataccgga tattgtattc aaatgtccac cctttaccat atccatattc
     1381 ttctgtaaac aagaaactgt tatagttaaa cctgatattc aggtcaaagc ccctggccgg
     1441 cacataaaaa tctgtacggg gaatgtatag attaccgctt aaggtattta cctgggtgcc
     1501 cagcggccct tgtttattgg ggttgttcag gttctgaccc aaagcctcca cagcgcaaac
     1561 tgaaaaacac accagcagca ttaacctggt cgtacgcatc ttcctggtgc aaatattttg
     1621 gatgctatgc ataaaatatt tttagctggc ttttattgaa gtttaaataa tactgatttg
     1681 tcttcgggaa cctgtggagg tgcgatacaa tgaacgataa ttgacccgcc accacctggt
     1741 cttaaaatac ccggaggacc atccggctcc gaaaattcta tatataatgt tgaagtgcca
     1801 ttaggaatac ctgccttggt aaaagccatc ttcacatctt catcattata taatatcctt
     1861 gtctgaacag gtaaatcaaa atttgttggg ttagtaaagt tgatgacaat aacaacaggt
     1921 ctgcccctta ctactttttc aggcacaatc agctccacca ctaattgtga gtttaacccg
     1981 ctggcggctc cgggatcaca gcctggtgca ttgccgtttc caggcccggt attaggccct
     2041 ccaccagtaa tcaatccacc gttgttggcg tttacaatgg aaaatccgtt cgccaacaca
     2101 gccgtagagg aatccggctt aatcagggtt agatcataaa ggccagtggg cttaccctgc
     2161 aaagggaatg tggcaaatac ttgtgtgttg tttgtataat aaacagcgga agcgtaaata
     2221 gtggtagttc cattactcaa tttggctatc atgctgtccc tgaataaaga tcctcgtatc
     2281 cgcactgtca cattacctat attgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc actcagtacg atttgctggt
     2461 taccatagtt gggagtttca aacctgtaat cataattggc cggtgtggga acaaatccac
     2521 cgcctatata catctcgttt cgtacaagaa gcgaatcatt tgtttttaac gtaacaagta
     2581 tagttgctcc ctgtaacgaa tcaggtatat gtaatttata atacctgttg actgtttgaa
     2641 gggtattcag ctctggtatg cccagcttca gttctt
//
LOCUS       DAORLS010000384         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_20865_length_2666_cov_2.405975, whole genome shotgun sequence.
ACCESSION   DAORLS010000384 DAORLS010000000
VERSION     DAORLS010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20865_length_2666_cov_2.405975"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PK977_18655"
     CDS             complement(<1..803)
                     /locus_tag="PK977_18655"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HRF20209.1"
                     /translation="MLFTRLHAFFNPEQFQGWGKKRNYFEGWYYKIVSSDQQKAFAFI
                     PGIAIDTNGYSHAFIQVLDGKQRTSLYHRFGIDDFNPSSKKLLVQLNKNSFSENHLQL
                     DLPGIKGELRFSGNVPWPKPLYSPGIMGPYAFAPFMECYHGIVSMDHSIQGALIIGNN
                     KIDFTNGRGYIEKDWGRSFPSAYFWMQSNHFSRPGISLKCSVAKIPWIRNSFTGFIAG
                     LWLNDQLYRFTIYNGTKLLRSYADENKVEIVLENRKCRLEILAHRDNAT"
     gene            875..2023
                     /locus_tag="PK977_18660"
     CDS             875..2023
                     /locus_tag="PK977_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HRF20210.1"
                     /translation="MIPTIHSKLPNVGTTIFTVMSALATEYKAVNLGQGFPDFPMSKE
                     LTDLVNKAMNDGFNQYSPMPGWMPLREAIAEKVQYLYHTAINPDTEITITPGGTYAIY
                     SAFTTILQPGDEVIVFEPAYDSYIPNIEINGAKAVTINLVYPDYHIDWEAVRKAINTK
                     TKAIIINSPHNPTGSVIGKKDIEELRKTVEGTNIFIISDEVYEHLIFDGIAHQSILRY
                     PDLLQRSFVCFSFGKVYNCTGWKLGCCIAPPELTKEFRKVHQFNCFSCHTPTQVALSH
                     FLKDKKAYQSLSGYMQGKRDYFTKLMQPTKFDMLESHGSYFICAKYDRISDENDKDFA
                     IRLTKEAGVATIPVSAFYHNGKDDKVLRFCFSKKEETLEQAVEKLLKV"
     gene            2026..>2666
                     /locus_tag="PK977_18665"
     CDS             2026..>2666
                     /locus_tag="PK977_18665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF20211.1"
                     /translation="MKDERYKGIFFMLLAALGFSLMGGAAKALKESFNAGQLVFYRNA
                     VGLIFLLPGLFIKPPQQQGGKPFRLAFRGMMGTVALYTLLYCILHIPLGTAMSYNLTS
                     ALFIAVFSYFLFNEYHGKRVGFAVLLGFAGMLLIYKPVMHLPWYYHLAGLISGITSAI
                     AYLTVNRLAYYYDPRVIVLSFLLSGFLVPLVTMIIHYTTGIHPDGLFIVDFKLP"
BASE COUNT          741 a          578 c          569 g          778 t
ORIGIN      
        1 tcagtggcgt tgtctcgatg cgccaggatt tccagccggc attttcggtt ctccagaact
       61 atttcaactt tattttcatc agcataagaa cgaagaagtt ttgtaccatt atatatggtg
      121 aagcgataca actgatcatt cagccaaaga cctgcgataa aaccggtaaa ggaattcctg
      181 atccagggga ttttggctac agaacatttc aatgaaatac ccggacggct gaaatgattg
      241 ctttgcatcc agaaataggc agacggaaat gaacggcccc agtctttttc aatgtaaccc
      301 cttccgttgg taaaatcaat cttgttatta ccaattatca gtgccccctg gatgctgtga
      361 tccatactta caataccatg gtaacactcc atgaacggcg caaaagcata agggcccatg
      421 atgcccggcg aataaagcgg tttgggccag ggaacgttac ctgaaaaacg taactcacct
      481 ttaatgccgg gcaggtcaag ctgcaaatgg ttttcagaga aactgttctt gttcaattga
      541 accagcagtt ttttggaaga aggattgaaa tcgtcaatgc cgaatcgatg atatagggag
      601 gttctttgtt ttccatccag cacctgaata aatgcgtggc tatagccatt ggtatctatt
      661 gctatgccgg gtatgaatgc aaaggctttt tgctgatcgc tgctgactat tttataatac
      721 cagccttcaa aataatttcg ctttttaccc catccctgga attgttcagg gttgaagaag
      781 gcgtggagtc tggtaaaaag catgattcaa aatacggaac attctgtatc tctgttgttc
      841 aatacacttt aattttactt catactatta atgcatgata ccaactatcc actctaaact
      901 acccaatgta ggcaccacca ttttcaccgt gatgagtgcc cttgctacag aatataaagc
      961 cgtaaacctc gggcagggat ttcctgattt tccaatgagc aaggaattaa cagacctggt
     1021 aaacaaggcc atgaacgatg gcttcaacca atactcaccc atgcccggct ggatgccatt
     1081 aagagaagcg attgctgaaa aagtacaata cctgtatcat acagccatca atcctgatac
     1141 agaaattaca attacacccg gcggcaccta tgctatctat tctgctttta ccaccatttt
     1201 acaacccggc gatgaggtga ttgtttttga accggcctat gatagctata tcccgaatat
     1261 tgagatcaac ggggcaaagg ctgtaacgat caatttggtt tacccggatt atcatattga
     1321 ctgggaagcg gtacggaagg ccattaatac aaaaacaaaa gccattatta ttaactcgcc
     1381 gcacaatcct accggtagtg ttatcggtaa aaaggatatt gaggagttaa gaaaaaccgt
     1441 tgagggcacc aatattttta tcatcagcga tgaggtgtat gaacacctga tttttgatgg
     1501 catagcgcat caaagtattt tacggtatcc cgacctgttg caaagaagtt ttgtttgttt
     1561 ctcttttggt aaagtatata actgcaccgg ctggaagttg ggctgttgta ttgcaccacc
     1621 tgaactgacg aaggaattcc ggaaagttca ccagttcaat tgctttagct gccacactcc
     1681 cacccaggtg gcgctgtcgc atttcttaaa agacaaaaaa gcataccagt ctttgtctgg
     1741 ttatatgcag ggcaaaagag attattttac caaactgatg cagccaacaa agtttgatat
     1801 gctggaaagc cacggcagtt attttatttg tgcaaagtat gatcgcatca gcgacgaaaa
     1861 cgataaagac tttgccatcc ggttaactaa agaagccggc gtggccacca ttccggtatc
     1921 agcattttat cataacggaa aagatgataa ggtgctgcgg ttttgctttt ctaaaaaaga
     1981 ggaaacactg gagcaggcgg tagaaaagct gctgaaagta taatggtgaa ggatgagaga
     2041 tataaaggta ttttctttat gctgctggcc gcattgggtt tttcgctgat gggtggtgca
     2101 gccaaggctt tgaaagaaag ctttaacgca ggccaactcg ttttttaccg caatgcagtc
     2161 ggattaattt ttttgttgcc cggtttattt atcaaaccac cccagcagca gggcggaaaa
     2221 ccatttcggc ttgctttccg gggaatgatg ggcacagtgg cgctgtacac tttattatac
     2281 tgcatactgc atataccgct tggtacggcc atgagctata atcttacttc tgctttattc
     2341 attgccgttt tctcttattt tctcttcaat gaatatcatg gaaaaagggt tggctttgca
     2401 gtattgctgg gctttgccgg catgttgtta atctacaaac ccgtcatgca cctcccctgg
     2461 tattatcacc tggccgggtt gatttccggt attacatctg ctattgccta tctcaccgtt
     2521 aaccggctgg cctactatta tgatccaagg gtgatcgtac tttctttttt actcagcggc
     2581 tttctagttc ccctggtcac catgatcatt cattatacta ccggtattca tcctgatggt
     2641 cttttcattg tcgatttcaa acttcc
//
LOCUS       DAORLS010000385         2641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21116_length_2641_cov_1.370070, whole genome shotgun sequence.
ACCESSION   DAORLS010000385 DAORLS010000000
VERSION     DAORLS010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21116_length_2641_cov_1.370070"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>255)
                     /locus_tag="PK977_18670"
     CDS             complement(<1..>255)
                     /locus_tag="PK977_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20212.1"
                     /translation="NPSTVFEVLSNGSGRVGLFQNTNAGSTAPALSAMSNGTGDAIQS
                     IMTGNGKAGLFTINNASNGNFALDVSSNGTNSVINSISTGT"
     assembly_gap    256..299
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<300..1265)
                     /locus_tag="PK977_18675"
     CDS             complement(<300..1265)
                     /locus_tag="PK977_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20213.1"
                     /translation="MRKLLLTTSFLFLLVISMIAQAPSFFNYQGVARNSVGNALVNKT
                     IKLRITIRDSNPLGLPLYSETKIVTTNAFGLFNVQVGAPPATNTTGSMSNINWNVNRK
                     WMQVEIDVENNGTFVDIGTTQLSSVPYSLHSNQSGDVILPFVKSQNEENPLMKITNTG
                     NNASSLAFEGLSNSTANNATAIRGIISSPSPGSFSAGITGQNNGTTGNGVGVYGSQNG
                     SGYGVYGTTPGGAGVYGNSTTGTGVYGQSVSGPSVMGYQPNTGTSNAGLFQNFSTTNA
                     ASVLRVLSNGLGEGLSVNMTGLGKGGVFAINNAANGNTGLEVSTNG"
     gene            complement(1328..>1835)
                     /locus_tag="PK977_18680"
     CDS             complement(1328..>1835)
                     /locus_tag="PK977_18680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20214.1"
                     /translation="SSQSITPMVINAGGGFYNNPSSYSRYFDWSIAELALINTVAAPD
                     STVIVYQGVLQPGAEKPGYTPFSANFNSEDVKLFPNPTIGKFEINFFVNAQGILDLEL
                     TDAMGRIIERRSMNYGGCCRIVHYDISNLPAGVYFIIATIRPDPFNSYNVGQQPRRSG
                     LRVVKMNP"
     assembly_gap    1836..1934
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1982..>2255)
                     /locus_tag="PK977_18685"
     CDS             complement(1982..>2255)
                     /locus_tag="PK977_18685"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF20215.1"
                     /translation="KFRLSEILFAEADNNYVNIHTAGQKFSLRLSLSELIERLQFQRL
                     VRVHRSYAVNIDEISSFDDQLIRIGKHEIPIGRNHRQEFLDYFNLR"
     assembly_gap    2256..2321
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2322..>2641)
                     /locus_tag="PK977_18690"
     CDS             complement(<2322..>2641)
                     /locus_tag="PK977_18690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF20216.1"
                     /translation="KLFSEETVDILLMDIHIHGDKDGVETATELMKMKQVPLIYLTAF
                     TDAKTVERVKHTNPSAFLTKPYNIDNVRIAIDLALHHFAETKAPAKVVPMEKPATAGE
                     KKPD"
BASE COUNT          675 a          548 c          498 g          711 t
ORIGIN      
        1 gtacctgtac tgattgaatt tataacacta ttagttccat tagagcttac atccaaagca
       61 aaattgccat tggaagcatt gttgatagtg aacaaaccgg ctttaccatt tcctgtcata
      121 attgattgta tggcatctcc agttccgttt gacatcgcac ttaaagcagg tgcagtgctt
      181 ccggcattgg tattttgaaa aagacctacc ctacctgaac catttgataa tacttcaaaa
      241 acagtactgg gatttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
      301 ccgtttgtag aaacttccaa acctgtatta ccattggccg cattattaat ggcaaacaca
      361 cctcctttgc caagtcctgt catattcacc gataaacctt caccaagacc gttacttaaa
      421 acccttaaga ctgaagcagc gttggtggta ctgaagttct gaaataaacc cgcattactt
      481 gtacctgtgt taggctggta acccataaca gatggtccgg aaactgattg accataaaca
      541 ccggtaccag tggtactgtt accatataca cctgctccgc cgggagtagt accataaaca
      601 ccatatccgc tgccattctg tgaaccataa acccccacgc cattaccagt agtcccatta
      661 ttctgacccg taataccggc tgaaaaagaa ccaggtgaag gtgatgaaat aataccccgg
      721 atggccgttg cattattggc agttgaattt gataatcctt caaaagcaag gctgctggca
      781 ttgttaccgg tgttggtgat cttcattaac ggattttctt cattctgtga ttttacgaac
      841 ggcaatatca catctccgga ttgattacta tgaagggaat agggaaccga acttaactgt
      901 gtagttccta tgtctacgaa agttccatta ttttccacat caatctctac ctgcatccac
      961 ttcctgttca cattccagtt aatgttgctc attgatccgg tagtatttgt tgcaggaggt
     1021 gctcctacct gaacattgaa caaaccaaaa gcattggtag taacgatctt tgtttcgcta
     1081 tacaatggca aacccaacgg attcgaatca cggatagtaa tccgaagctt gatggtttta
     1141 ttcaccagtg cattgcctac cgaattacgg gccaccccct ggtaattaaa aaatgacggc
     1201 gcctgtgcta tcatgcttat gacaagcaga aacaggaaag atgttgttaa taatagcttt
     1261 ctcataatag ttactttgag tttgttgaac ttgtcttcaa aaaggtttaa tggtttatgg
     1321 tatttgatta tggattcatt ttaactaccc tcaggccgct tctccgcggt tgctgaccta
     1381 cgttatagct attaaaagga tcaggtcgaa tcgttgcgat aatgaagtaa actcctgcag
     1441 gcaggttgct gatatcataa tggacaatgc ggcaacagcc accgtaattc atacttctcc
     1501 gctcaataat gcggcccatg gcatcggtaa gctccaggtc aaggattccc tgcgcattga
     1561 caaaaaagtt gatttcaaat tttccaatcg ttgggttagg aaacagcttt acgtcttcac
     1621 tgttgaaatt ggcagaaaac ggagtataac ccggcttttc agcacccggt tgtaataccc
     1681 cctggtatac aattacagta ctgtctggtg cagccactgt attaatcagg gcaagttcgg
     1741 caatgctcca gtcaaagtac cttgaatagg aagacgggtt attataaaag cctcctcctg
     1801 cattaatcac cattggagta atgctttgag aagaannnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnngtatgt gaacgaccgt cttttgtatg tgaatgaatc ctcttacaag
     1981 gtcagcgcag gttgaaataa tccaggaact cctgccggtg gtttcttccg ataggaattt
     2041 catgtttgcc aatacggatc agttgatcgt caaatgaact gatctcatca atattgaccg
     2101 cataggagcg atgtaccctg acaagccgct gaaactgaag ccgctcaatc agctcactaa
     2161 gggagagccg aagggaaaac ttctgcccgg cagtatgaat attgacgtaa ttattatctg
     2221 cttctgcaaa gagtatttca gaaagcctga atttgnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatccggtt tcttctcccc
     2341 ggcagttgcc ggcttttcca ttggcactac tttggccggg gcttttgttt ccgcaaagtg
     2401 atgcagggcc aggtcaatag cgattcttac attatctata ttataaggct tggtcagaaa
     2461 agctgatggg ttggtatgct ttacccgttc cactgttttg gcatctgtaa aagcagtgag
     2521 atagatcaac ggcacctgtt tcattttcat cagttctgtc gctgtctcca cgccgtcctt
     2581 atcgccgtga atatggatat ccatcagcag aatatcaacg gtttcttcag aaaacaattt
     2641 c
//
LOCUS       DAORLS010000386         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21258_length_2629_cov_2.851204, whole genome shotgun sequence.
ACCESSION   DAORLS010000386 DAORLS010000000
VERSION     DAORLS010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21258_length_2629_cov_2.851204"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..>2404)
                     /locus_tag="PK977_18695"
     CDS             complement(197..>2404)
                     /locus_tag="PK977_18695"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF20217.1"
                     /translation="VKAQQLYTESGNIWKSELGTDHPDYALSLNNLASVNEVTKNYAS
                     AESLYLQALTIRKRVLGEDHQDYAMTLNNLASLYATQKQYDKALPLLTQASATWKRTV
                     GENHPSYATSLNNLAALYRKFKKNYGEAEKLYNQSITIRKKSLGENHPFTAETQADLA
                     LLYIQMEQYKKAEPLLIQSNLVLQKNVADVFTILSEEEKANYLSYNLSAMDINNSLLY
                     NYPSAGKNFIKSSLELLLGYKSLSLSATKNMLESVRSSSDTALKRLLQAWITQKKILS
                     KQYTLPVEFRTKNLKGIEAEAEATEKEIIRKSSSFKSQATSLHVTMREVQTNMTEDEA
                     AIEFLHFKLYRYGKTDSSIYAAYLFRKHDSVPVFVPLCEKQALKKLFDSAGNSTTKMV
                     SSFYRGIEIKNTSSLLGQSLYKLIWAPLEPHLKGIKKIAYSPSGKLYAIALHALMADS
                     NTLLMDKYQLQQYTSTRQIAFRTETRASPPGDIVLFGDADFSMDSLQLTRLRKDNTVS
                     VPVTHSVFQSTSTWPGLPGTGAEVINIKQLFDQSKVKARVFTKVNATEENLKAISNTG
                     YKEIHIATHGFFLPEPEENKTASISKNQYRKAEDPLMRSGLILAGGNYVWSGKAPITG
                     VEDGVVTAYEISQLNLSNAELVVLSACETALGDVKGSEGVFGLQRAFKMAGVKKMIVS
                     LWQVPDKETAELMTVFYTYWLKGKNVNDAFYQAQADMRKKYTPFYWAAFVLVE"
     assembly_gap    2405..2417
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2418..2597)
                     /locus_tag="PK977_18700"
     CDS             complement(<2418..2597)
                     /locus_tag="PK977_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF20218.1"
                     /translation="MTQLNNLAGLYQSMGLYTKAEPLYIEARDIRKKMLGEKHPRYAM
                     SLNNLATLYQEMGQYV"
BASE COUNT          650 a          563 c          522 g          881 t
ORIGIN      
        1 gaaggaaatc agcagaaagc caagatccac cataggtgtc atatctactt ttgggcaatg
       61 ctttatcatt cttcccactc caactctttt tctcagttgt atttcactgt tgctgattat
      121 actattaaat tgtgccataa gctctggttt gcttataaga cacttgttat tattatttcc
      181 acaaatcaaa ctggttttac tctaccagta caaatgccgc ccaatagaaa ggagtatatt
      241 tttttcgcat atcggcctgc gcctggtaaa atgcatcatt tacatttttg cctttaagcc
      301 agtaggtata aaatacagtc attaactctg ccgtttcctt atccggcacc tgccaaaggc
      361 ttacaatcat cttttttaca cctgccatct tgaaagccct ttgcaggcca aaaactcctt
      421 cgcttccttt tacatcaccc aacgctgttt cgcaggcact tagcaccacc agctctgcat
      481 tagacagatt gagttgtgaa atttcatagg cagtaacaac accatcctcc actccggtga
      541 taggagcttt accgctccat acataattac ctccggcaag gatcaatccg cttctcatca
      601 aaggatcttc tgcctttcta tactgatttt tagaaattga tgccgtctta ttttcctctg
      661 gctctggtaa aaagaatcca tgcgttgcaa tatgaatttc cttatacccg gtattactga
      721 ttgctttcag gttttcttcc gtagcattga cttttgtaaa taccctggcc ttcactttgc
      781 tctgatcgaa caattgtttg atattgatca cttctgcccc ggttccgggc aggccgggcc
      841 atgttgatgt actttgaaaa acagagtgtg ttacaggtac cgatacggta ttgtcttttc
      901 gtaaacgagt taactggagg ctgtccattg aaaaatctgc atcgccaaat aacactatat
      961 ctccgggtgg cgatgccctt gtttcagtac gaaatgcaat ctgccttgta ctggtatatt
     1021 gttgcagctg atacttgtcc attaaaagcg tattgctgtc agccataagt gcatgtaagg
     1081 caatggcata taatttaccc gaaggggagt atgctatttt tttaatgccc ttcaggtgcg
     1141 gttccagcgg ggcccaaata agtttataaa gactttgccc aagtaaggag gaggtgtttt
     1201 tgatttcgat accccggtaa aaactgctta ccattttagt tgtagaattt cctgcactat
     1261 caaaaagctt ttttaatgcc tgcttttcac ataatggtac aaagaccgga acggagtcat
     1321 gttttcggaa caggtatgct gcatagatgg acgaatctgt ttttccatat ctgtataatt
     1381 taaaatgaag gaattctatt gccgcctcat cttctgtcat attggtttga acctcccgca
     1441 tggttacatg gagtgaggtt gcctggcttt taaaagagga tgactttctg atgatttcct
     1501 tttctgtagc ctcggcttct gcttcaatac ccttcaggtt ttttgtcctg aattctaccg
     1561 gcaatgtgta ttgttttgaa agtattttct tttgggtgat ccaggcctga agtagtcttt
     1621 taagcgctgt gtcgctgctg cttctaactg actcaagcat gttctttgta gccgacagcg
     1681 aaagcgattt atagcccagt agcaattcaa gactgctttt aataaaattt tttccggctg
     1741 aaggataatt atatagcagg ctgttattga tatccatagc cgaaagatta taagacaggt
     1801 agtttgcctt ttcttcttca gacaaaatgg taaaaacatc agctacattt ttctgtaata
     1861 ccaggttgct ttgtatcagt agtggctctg cttttttata ttgctccatc tggatataca
     1921 gtaatgccag gtctgcctgt gtttcggcgg taaacggatg attttctccg aggctctttt
     1981 tacggattgt tatcgactgg ttatacaatt tttcagcttc accatagttc tttttaaatt
     2041 ttcggtacaa agcagcgagg ttattaagac ttgtagcata cgaaggatgg ttttctccca
     2101 ctgttcgttt ccaggtagcg gacgcctggg tcaataacgg aagtgccttg tcgtattgtt
     2161 tctgcgtggc ataaaggctg gccaggttat tcagcgtcat tgcataatcc tggtgatctt
     2221 cccccagaac cctcttgcgg atagtaagtg cctgcaggta caaactttcc gccgaagcat
     2281 aattcttggt gacttcattg actgaagcaa gattattcag acttaaagca taatccggat
     2341 gatcggtacc cagttctgat ttccagatat tgccggattc tgtatacagt tgttgtgctt
     2401 tgacnnnnnn nnnnnnngac atattgcccc atttcctggt acagtgtagc aagattgtta
     2461 aggctcatag catagcgggg atgtttttca cccagcattt tttttcgaat atctcttgct
     2521 tcaatatata agggttctgc cttcgtatat aagcccattg actggtacag acctgccagg
     2581 ttattcagtt gcgtcaaata atccggatgg tttttaccca gattttttt
//
LOCUS       DAORLS010000387         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21454_length_2610_cov_1.980822, whole genome shotgun sequence.
ACCESSION   DAORLS010000387 DAORLS010000000
VERSION     DAORLS010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21454_length_2610_cov_1.980822"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PK977_18705"
     CDS             complement(<1..602)
                     /locus_tag="PK977_18705"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF20219.1"
                     /translation="MKKYLLLYTIAILLVKQSVGQANLSANSLENKAVSILNVTLTGK
                     ITDSKTGEPLAGASIYLADDRIGTYADANGKYMLTNIPFGHHVMEVSYTGYTTMVEHI
                     QLTADTEKNFALSAMIMENQGVVVTGVAGATSIRKIPVPVNSIRRSALLQLPSSNIID
                     ALTHIPGVSQLSTGPAISKPFIRGLGYNRVVTISDGVRQEG"
     gene            complement(683..1009)
                     /locus_tag="PK977_18710"
     CDS             complement(683..1009)
                     /locus_tag="PK977_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20220.1"
                     /translation="MKSKGQIGYRKIIAAIFFAALAFVYAEKVMHKHECPLVETNQAS
                     VSKNLEYTTCTLCDFQPVTAAEVPVIAECEVPVKFIFHKFIPESDNYFYQSLVLINGR
                     GPPRFS"
     gene            1187..1783
                     /locus_tag="PK977_18715"
     CDS             1187..1783
                     /locus_tag="PK977_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20221.1"
                     /translation="MFQTNLQYRYPALFLSLFIVLVISGCSKKDSPAAPDPCIGVSYD
                     VQYFKTESIGTSNNGSITINYPVGDTITYKLNNGSYQAAPIFNNLAPGDYVVTVKNQK
                     GCTDTAQISILNYGPKYALVKQIIVGYCGPCHRNGGNEGGKNFDTDASIVASWDRIKA
                     RAVDANPSQMPQAPNAPLTNPDKQKIVDWVNAGHRQSD"
     gene            complement(1851..>2610)
                     /locus_tag="PK977_18720"
     CDS             complement(1851..>2610)
                     /locus_tag="PK977_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRF20222.1"
                     /translation="MVSFGGFDTHSVQVNAADTTTGAHATLLQRVSDAIKAFQDDLKF
                     LGVEERVIGMTYSEFGRRIKSNSSVGTDHGAAAPMFLFGSSVESGMLGVNPTIPANAS
                     VNDNIPMQYDFRSIYSTILEKWFCLDKTIVESLFPPNINTQLQTLPLLKAGVCSGVTP
                     PPPPDPTDVIVTNYPNPFTTSTTIKFKTEGGHTLIQIIDTLGRVLKVLVDRDYPTAGI
                     YTVNFEAEGLPFGIYYARFQNGVTQQVRAMLKVR"
BASE COUNT          760 a          558 c          538 g          754 t
ORIGIN      
        1 ccctcctgcc ttactccgtc gcttattgtt acaaccctgt tgtagccaag acctcgtatg
       61 aaaggtttgg aaatggcagg accggtggat aattgggata caccgggtat atgcgttaat
      121 gcatcaataa tattagaaga aggaagttgt agtaatgccg aacgacgaat tgaattaaca
      181 ggcacaggta tcttccgtat actggtggca ccggcaacac ctgttaccac taccccctgg
      241 ttttccataa tcatagccga caaagcaaaa ttcttttcag tatcggccgt cagttgaata
      301 tgttccacca ttgttgtgta accggtatat gaaacttcca ttacatggtg tccaaatggt
      361 atgtttgtaa gcatatactt tccgtttgca tcagcataag tgccgattct gtcatcggca
      421 agatatatag atgctccagc cagtggctcc cctgttttag agtcggtaat tttaccggtg
      481 agtgtaacat ttaaaattga aacagctttg ttttcaagag aattggctga caggtttgcc
      541 tggccaacag attgctttac taacagtata gcgatagtat ataataataa atattttttc
      601 atcagactta aaaattgaat aggcaccggt ttcacagccg gctccggatt gaaaaatggt
      661 actaaagaga aagaaaaaag gttcaggaaa accggggtgg tcctcttcca ttaataagta
      721 ctaacgactg gtagaaataa ttgtcacttt ccggaatgaa tttgtgaaat ataaatttca
      781 ccggcacctc acattctgca attacaggta cctcagctgc tgttacaggt tgaaaatcac
      841 aaagagtgca agtagtgtac tctaaatttt ttgatacgga agcctggttc gtttcaacta
      901 aaggacactc atgtttgtgc atgacttttt cagcatacac aaaggccagg gccgcaaaga
      961 atatggccgc aatgattttt ctgtacccta tttgtccctt acttttcacc ggtgcgaaat
     1021 tagggaattt taattatttg caacattgat tcaattaatc aacccgatta ttactcattt
     1081 tcttgaaaaa atactgatgc tgctcctttg gtttaatctt tgaagcaatt aatcagtctg
     1141 atgactgctc acttttcctg attgtttatt tgtattaaat tagcttatgt tccaaaccaa
     1201 cttgcaatac cgctaccctg ctttatttct gtcattgttt attgttttag taatatcagg
     1261 ctgttcaaaa aaagatagtc ccgcagcacc cgacccttgt attggggttt cttatgatgt
     1321 tcaatatttc aagacagagt ctatcggaac atctaataac ggaagcatta ccattaatta
     1381 tcccgttggt gataccatca cttataaact aaataatggt tcttaccagg ccgctcctat
     1441 ttttaataac cttgccccgg gtgactatgt agtaacggta aaaaatcaga agggatgtac
     1501 agacacagcg caaatcagta tattgaatta tgggcctaag tatgcattgg taaaacagat
     1561 cattgtgggt tattgtggcc cctgtcaccg taacggggga aatgagggcg gaaagaactt
     1621 tgataccgat gcaagcattg ttgccagctg ggaccgcatt aaggccaggg cagttgatgc
     1681 aaatccatca caaatgcctc aggccccgaa tgcaccgctc actaaccctg acaaacaaaa
     1741 aattgttgac tgggtaaatg ccgggcaccg gcagagtgat tagaagaaat taaatgctaa
     1801 gcaaaaggcc ggatgatccg gcctttatta ttaattgtaa taaagtcaaa tcatctcact
     1861 ttcaacatgg cccttacctg ctgggtaacg ccattctgaa aacgggcata ataaattcca
     1921 aatggcaatc cctctgcttc aaaattgacg gtataaatac cggcagtagg ataatccctg
     1981 tccactaata ccttcagtac acggcccaat gtgtcaatta tctgtatcag cgtatgcccc
     2041 ccttccgttt tgaattttat ggttgtactt gttgtaaacg gattgggata gttggtaaca
     2101 atgacatctg tggggtcagg aggaggtggc ggtgtaacac cactgcacac acctgccttt
     2161 agcaacggaa gggtttgcaa ttgtgtgttg atgttgggag ggaataatga ctctacaatc
     2221 gttttatcca ggcagaacca tttttccaaa atagtcgaat agatactcct gaaatcgtat
     2281 tgcatgggaa tattgtcgtt gaccgatgca ttggcaggta ttgtcgggtt caccccaagc
     2341 atgcccgact ctacgctgct gccaaataag aacataggtg ctgccgcacc atgatccgtt
     2401 cccacactgg aatttgattt gatgcgtctt ccaaactcac tataggtcat accgatcacc
     2461 ctctcctcta cccctaaaaa cttaagatca tcctggaaag ctttgatagc atccgaaacc
     2521 cgctgcagca aggtagcatg tgcaccagta gtggtatcag ctgcattaac ctgtacagaa
     2581 tgggtatcaa agccgccaaa actaaccatg
//
LOCUS       DAORLS010000388         2608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21475_length_2608_cov_1.960439, whole genome shotgun sequence.
ACCESSION   DAORLS010000388 DAORLS010000000
VERSION     DAORLS010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21475_length_2608_cov_1.960439"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PK977_18725"
     CDS             complement(<1..1289)
                     /locus_tag="PK977_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20223.1"
                     /translation="MPKLNSFKGKALITALLLLLNFRSPAQKVKLGISYASSIYSNKE
                     RFEFTNGEQTIVLDFQHPAVYFSKEYQAGQPYSISQLSGPRPVTFLDQQRGTMGNEDK
                     LLMANCGHPPLTIYKLKVIGVEQGETFKFSDNYRRTYNIPISTTTNLGGYPRGDDYLI
                     SQTEGPRQCRLSFNQGIVGENSITVQADCRKVTSTNPPAPQHRFDLVTRSSDNKIFNT
                     YYESSMPVIGGKGEDEARYVAFVMYGKNIDGSNGNYRQIFWRDRKAGITKLISKSATG
                     EQGNGNSFSPSISTDGRFVVFETYATNFSASDNNAVRDIYLWDKTTEQLTLISKSISG
                     TTANGESYEPVISGDGSTIAYTSNASDIVKLEPVFNTPNVYVCDVNGGSTAFITKDFE
                     TGKAAGGYAPSISDDGTKIAFCAYTSRLVKNDVNNLW"
     gene            complement(1379..>1752)
                     /locus_tag="PK977_18730"
     CDS             complement(1379..>1752)
                     /locus_tag="PK977_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF20224.1"
                     /translation="KDLYSFTPMPNDGAKQPKLKGQVCYGWDLSGSTSEVLNLVDDKV
                     QIKWLLEAYKLFPQKDSFFILPKSGNMEQSFFTKLAGNNELWQQIRSGKTEDDIRKTW
                     EPKLGEFKLIRKKYLLYEDFE"
     assembly_gap    1753..1775
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1776..>2230)
                     /locus_tag="PK977_18735"
     CDS             complement(<1776..>2230)
                     /locus_tag="PK977_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF20225.1"
                     /translation="SSLEEFMEAAFELKKPLLLLDRPNPNGHYVDGPVLDKKYKSFVG
                     MQPVPIVYGMTIAEYAMMLTGENLLSEKANAYANYFKTVQQHHLDTPFHFQIIKCQNY
                     THKSKYVLPIKPSPNLPNIQSIYLYPSTCFFEGTVLSEGRGTDKQFQVF"
     assembly_gap    2231..2361
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2362..>2608)
                     /locus_tag="PK977_18740"
     CDS             complement(<2362..>2608)
                     /locus_tag="PK977_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF20226.1"
                     /translation="PPPPPPPPPPPAVQLKITAGAERVNVYLPLIKGKKIGVFANQTS
                     MVGNTHLVDTLRSLGIDIKVIFGPEHGFRGTADAGEKV"
BASE COUNT          643 a          513 c          492 g          806 t
ORIGIN      
        1 tcccacaggt tattgacgtc attttttaca agccggctag tataggcaca aaaagcaatt
       61 ttcgttccgt catccgaaat agagggtgca tatccgccag ctgctttccc ggtttcaaaa
      121 tcttttgtga taaacgctgt gctgccgcca ttaacatcac atacatagac attcggagta
      181 ttaaatacag gttccagttt tacaatatcg ctggcattgg aagtataggc aattgtactt
      241 ccgtcgccgg aaataaccgg ctcatagctt tcaccgttag cagtagttcc tgagattgac
      301 ttgctaatta gtgtcagttg ttctgttgtt ttatcccata ggtaaatatc acgaacggca
      361 ttattatcgc ttgcagaaaa attagtagca taagtttcaa atacaacaaa acggccatct
      421 gttgatatag aaggcgaaaa gctgttccca ttgccctgct cgccagttgc tgatttgctt
      481 attaattttg ttatacctgc ttttctgtct ctccaaaaga tctgccggta attaccattg
      541 gaaccgtcaa tattttttcc atacatcaca aaagcaacat aacgggcttc atcttctcct
      601 ttaccgccta ttactggcat tgagctttca taataagtat taaaaatttt attgtcacta
      661 cttcgggtca ctaaatcaaa ccgatgctgt ggtgccggcg ggtttgtgga ggtaacttta
      721 cggcaatctg cctgcacagt aatgctgttt tctccgacaa taccctggtt aaagcttaat
      781 ctgcattgtc ttggtccttc agtctggctg ataaggtagt catcacctct tggatatcct
      841 cctaaattag ttgttgttga aattggaata ttatatgttc tccggtaatt atcactaaac
      901 ttgaaagttt caccctgctc tacacctata acttttaatt tataaattgt taacggaggg
      961 tgaccgcaat ttgccatcaa taatttatcc tcgttcccca tcgtacctct ttgctggtcc
     1021 agaaatgtca ctggtctagg gccggataat tggcttatac tataaggttg ccctgcctga
     1081 tattcctttg aaaaataaac agcaggatgc tgaaaatcca atacaattgt ttgctctcca
     1141 ttggtaaatt caaatctctc tttattggaa taaatgctgc tggcatagga aattcctagt
     1201 ttaacttttt gtgcagggga gcggaaattc agtaaaagaa gcaaagctgt aattaaggct
     1261 ttgcctttaa acgaatttag ttttggcata gaaatgcggt ttaattttga attagtaaat
     1321 caccgtcttt ttttattctg attactttgt cgatttcctt ctgggctcag catgttgctt
     1381 actcaaaatc ttcatatagc aggtatttct ttcttatgag tttaaattcc cccagctttg
     1441 gttcccaggt tttcctgata tcatcttcag tttttccgga tctgatctgc tgccacaatt
     1501 cattattccc tgccagcttg gtaaagaatg actgttccat gttgccagat ttgggcaaaa
     1561 taaagaaact gtctttttga ggaaatagtt tatatgcttc caacagccac tttatttgca
     1621 ctttatcatc caccaggttc agcacttcgg atgtactgcc actaagatcc catccataac
     1681 aaacctgtcc ttttaattta ggttgcttgg caccgtcatt gggcatgggt gtaaatgaat
     1741 aaagatcttt tgnnnnnnnn nnnnnnnnnn nnnnncaaat acctggaatt gtttgtcagt
     1801 acctcttcct tcacttaaaa ccgttccttc aaagaagcag gtggaggggt acaggtatat
     1861 tgattgaatg ttcggcaggt tgggggaggg tttgatgggc agcacatatt tactcttgtg
     1921 cgtatagttc tggcatttta taatctggaa atgaaatggg gtgtccaggt gatgctgttg
     1981 tacggttttg aaataattag cataggcatt ggccttttca gaaaggaggt tttcaccggt
     2041 cagcatcatg gcatattcgg caatggtcat tccatataca atggggactg gttgcatgcc
     2101 aacaaaagat ttatactttt tatccaatac cggcccgtct acataatgac cattgggatt
     2161 aggcctgtcg agtaacagca atggcttttt caattcaaag gcagcctcca taaattcttc
     2221 cagcgacgaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn ncaacttttt cgcctgcatc agcggtgcct ctaaaaccat
     2401 gttcgggtcc aaaaatcact tttatatcaa tgccaaggct tcttaatgtg tcaaccaggt
     2461 gagtattgcc caccatcgag gtttgattgg caaaaacacc tatttttttc cctttgatca
     2521 atggcaggta aacgttcact ctctcagctc ctgcagttat tttcaactgt actgccggtg
     2581 ggggtggtgg tggcggcgga ggaggagg
//
LOCUS       DAORLS010000389         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21491_length_2607_cov_1.738636, whole genome shotgun sequence.
ACCESSION   DAORLS010000389 DAORLS010000000
VERSION     DAORLS010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21491_length_2607_cov_1.738636"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..907
                     /locus_tag="PK977_18745"
     CDS             110..907
                     /locus_tag="PK977_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20227.1"
                     /translation="MKKYFILSIVSLFFLHSDAQERYRINSGLYYGGDFYEGSKTAGY
                     YWFGKDSFLVMASFRKDSSLVSFGIGKYYVTKQNLLIRIFEKVPELTLKSNKVEYYLS
                     TITPTDSLFFRGTIFDSNDRPVKIGIIFFENQENKKYRTDTNGNYSFAIPISQYPSKI
                     IYNSVYGCPLSLELVPNYNLYRINVSLNNDCLSGSIVSTFAYEVIKEKRDTSKIKKIE
                     NNTIRFAGPVKFERIAENNQILIAFLEKAKIRQPHLALLINWVANKL"
     gene            1019..1747
                     /locus_tag="PK977_18750"
     CDS             1019..1747
                     /locus_tag="PK977_18750"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRF20228.1"
                     /translation="MITTLCFDFGNTRKKVAVFQDAEMKEAVTMPDDSLDTIQSLIKK
                     YNPQKSILSSVIDHNVQMEEALAAATKFHKLSHHTKVAFTTPVGKPETIGADRLALAA
                     AAVHFYPGKNNFIIGLGTCITYNFINKYNELVGGAISPGMEMRFKALNYYTAKLPLIK
                     ADSNLPLIGYDTATNILSGVVLGMAKEIDGFIEAYREKFRNFNVVLTGGDTLHLASHL
                     KNKIFADPDLIFKGLYAISEVNNP"
     gene            1719..>2607
                     /locus_tag="PK977_18755"
     CDS             1719..>2607
                     /locus_tag="PK977_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20229.1"
                     /translation="MQLVKSTTPKKIKTLYCLILTILVSFFAVFEPVSAQDNSPYSRY
                     GIGDLVPPTNIISRGMGGVSAGYADLLSINFNNPATYASFQSVKEAKSKKLSSGRAIL
                     DLGLNFDTRTLREPANNNKFVANNALFSYVQVGVPLKQGWGLSFGIRPVSRISYKIFR
                     TEMLKDPLTGLPIDTATTVFNGDGGSYLASMGTGFTVFRKEKHGLIEQLNVGFNAGYL
                     FGEKDYSTKRYFINDTVSYNQANYETKTNYGNLYFNAGLQYLLPLNKKLVLTIGAFGA
                     WGQKMNATQDILRETYLFNE"
BASE COUNT          818 a          468 c          543 g          778 t
ORIGIN      
        1 cagcgtattc taaatgcggt taatgcagaa aaggatccat atggggaaaa tgcaaatgta
       61 aataatcaac aggcaaaggg tgatccatgt gattaaaata ggatttatta tgaaaaagta
      121 tttcattctt tctatcgtaa gcttgttttt tctacactcg gatgcacaag aaaggtatcg
      181 tattaatagc ggcctgtatt acggggggga tttttatgag ggatcgaaga ctgcggggta
      241 ttattggttc ggtaaagatt cctttttagt aatggcttca tttcggaagg atagtagttt
      301 ggtgtctttt ggtataggaa aatattatgt tacaaagcaa aatcttttaa ttagaatttt
      361 tgaaaaagtt cctgaattga ccttaaaatc aaataaagtt gaatattatt taagtacaat
      421 tacaccaacg gatagccttt tttttagagg aacgatattt gattccaatg ataggccagt
      481 taaaatagga ataatttttt ttgaaaatca ggaaaacaaa aagtacagaa cagataccaa
      541 tggtaattat tcatttgcta ttccaatatc acaatatcca tcaaaaatta tttataattc
      601 agtttacgga tgtccgttgt ctttagagtt agtaccgaat tataatttat atagaattaa
      661 tgtgagtctg aataatgatt gtttgtcagg aagtattgtt tctacatttg cgtatgaagt
      721 tataaaagag aaaagggata cctcaaaaat taaaaaaatt gagaataata ctattcgctt
      781 tgcaggtcct gtaaagtttg aaagaattgc agaaaacaac caaatactaa tagctttttt
      841 ggagaaggca aagatcaggc aaccgcattt ggcattatta ataaactggg tggctaataa
      901 actgtagaat tgattttttt aagacatcat taccatgagg gttgaattgc tgcgtaagca
      961 gacacaatta ttttagagag tttggaaaac aagtaacgat acatagtatt tttgatccat
     1021 gatcaccacc ctttgttttg attttggcaa cacccggaaa aaagtagctg tctttcaaga
     1081 tgcagaaatg aaggaggcgg ttacaatgcc ggatgattca ttagatacta ttcaatcgct
     1141 cataaaaaaa tacaacccac agaaatccat tctttcgtct gtgattgatc acaatgtgca
     1201 aatggaagaa gcccttgctg ccgctacaaa atttcacaag ctgagtcatc atactaaagt
     1261 agcctttacc acaccggttg gtaaaccgga gaccatcgga gcagacagac tggccctggc
     1321 tgctgctgcg gtacacttct atccgggcaa gaataatttc attattgggc taggaacctg
     1381 cattacctac aatttcatta ataaatacaa tgagctcgta ggaggcgcca tttctcccgg
     1441 tatggaaatg cggtttaaag cgctgaatta ttatacggcc aagctgccat tgataaaggc
     1501 agacagcaat ttgccgctga ttggctatga tacagctacc aatattctgt ccggcgtggt
     1561 gctgggaatg gccaaggaaa tcgatggttt tattgaggcc tacagggaga agttccggaa
     1621 ctttaacgtg gtcttaaccg ggggcgatac cctgcatttg gcttcacacc ttaaaaacaa
     1681 gatatttgca gaccctgatt taatctttaa aggtctgtat gcaattagtg aagtcaacaa
     1741 cccctaagaa aatcaagacg ttatactgct taatcttaac gatcctggtt tcttttttcg
     1801 ctgtttttga gcctgtttca gcccaggata attcacctta ttcccgttat ggtataggcg
     1861 acttagtgcc gcctaccaat attatcagtc ggggtatggg aggtgtttct gccggttatg
     1921 cagatctttt atcgatcaat tttaataacc ctgccactta tgcctctttt caatcagtga
     1981 aagaagccaa atcaaaaaag ctgtcatccg gccgtgccat cctggacctg ggattgaatt
     2041 ttgatacccg tacgctgagg gagccagcca acaataataa gttcgttgcc aataacgctt
     2101 tgttctctta tgtacaggta ggtgtaccgt tgaaacaagg ctgggggctg agtttcggta
     2161 tcaggcctgt ttcccgcatc agctataaaa tatttcgtac cgagatgctg aaagatcccc
     2221 tgacaggatt gccgattgat accgctacca cagtgtttaa cggagatggc ggttcttacc
     2281 tcgcttctat gggaacgggt ttcaccgttt ttcgtaaaga aaaacatggc ctgatcgaac
     2341 aacttaacgt aggtttcaat gcgggttatc tttttggcga aaaggattac agtacaaaaa
     2401 ggtattttat caacgatacg gtaagttata accaggccaa ttatgaaaca aagaccaatt
     2461 atggcaatct ttacttcaat gcaggcttgc aatacctgct tccgctgaac aagaaactgg
     2521 tacttactat tggtgcattt ggagcatggg gtcaaaaaat gaatgccaca caggatatac
     2581 tcagggaaac atacctgttt aacgaaa
//
LOCUS       DAORLS010000390         2599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21580_length_2599_cov_1.915881, whole genome shotgun sequence.
ACCESSION   DAORLS010000390 DAORLS010000000
VERSION     DAORLS010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21580_length_2599_cov_1.915881"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>356)
                     /locus_tag="PK977_18760"
     CDS             complement(<1..>356)
                     /locus_tag="PK977_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20230.1"
                     /translation="ENLQSDGSEQRRQWKALQAKDLFERSLKINPDNDSSKIGLGACY
                     LFGNISSNPLEGIAKIKEVVDRDSTNVYGQLTLVKGSLLSGQYDKAISRLETVTRLQP
                     TNIEAILLLADTYERT"
     assembly_gap    357..390
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<391..756)
                     /locus_tag="PK977_18765"
     CDS             complement(<391..756)
                     /locus_tag="PK977_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20231.1"
                     /translation="MKRPQWITLAAAVVLTAVLYVFGKTVPAKKTVAAPVDQHSPDDG
                     HNHGAMSLDSILIMAKKQLKPAQVAFLDGLEAAVAKEKVKEEQVHIYHQLAKYWADTA
                     RIFEPYAWYTGEGARLENSE"
     gene            988..>1452
                     /locus_tag="PK977_18770"
     CDS             988..>1452
                     /locus_tag="PK977_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20232.1"
                     /translation="MRLLLPTLLFTLFLFITSCGNKSKKQTEQTDISLIKDSLSSRQA
                     RADTTLAAIMPIIEKELNAWIKSFNGFSTDSFKYTQTTKFEEIAYQDTTGMNKFYELY
                     KPSLVFSGDSSQFIDIYSAGIMLEKKGKKIVASSDVDQAITLCNLQTGDWRQI"
     assembly_gap    1453..1587
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1679..1999)
                     /locus_tag="PK977_18775"
     CDS             complement(1679..1999)
                     /locus_tag="PK977_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRF20233.1"
                     /translation="MRKADLVNQISEKTGIPKVDVLVTLETMFKEVKDTLAGGENIYI
                     RGFGSFITKKRAAKIGRNIKKNIAVHIPEHYIPAFKPAKEFVAEVKKLKEPKPDTGPG
                     EDGE"
     gene            2187..>2599
                     /locus_tag="PK977_18780"
     CDS             2187..>2599
                     /locus_tag="PK977_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRF20234.1"
                     /translation="MKSKQKFSSQLLNWNKNQNKRKMPWKGEKDPYKIWLSEIILQQT
                     RVEQGLAYYNNFINTFPTVHHLAKAPDEKVFKLWEGLGYYSRCRNLLATARFISEELK
                     GIFPDSYESIKALKGIGPYTAAAISSFAFNLPHAVV"
BASE COUNT          686 a          544 c          489 g          711 t
ORIGIN      
        1 cggtacgttc ataagtatca gccagcaaca gaatcgcttc aatattagtg ggttgaagcc
       61 gggtaactgt ttctaaacga ctgatcgctt tatcatattg ccccgataat aatgaaccct
      121 ttacgagagt aagctggcca tatacattgg tactgtcacg gtccaccact tcttttattt
      181 tggctatacc ctccagcggg ttgctggaaa tattgccaaa caggtaacag gcaccaaggc
      241 ctattttgga tgaatcatta tccgggttaa tcttcaaaga tcgctcaaat aaatctttgg
      301 cttgcagggc tttccattgt ctccgctgtt ccgatccatc gctctgcagg ttctccnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttctgaattt tccaatctgg ccccttcgcc
      421 ggtgtaccag gcatagggtt caaagatccg ggccgtatcc gcccagtatt tggccaattg
      481 gtggtaaata tgcacctgct cttcctttac tttctcttta gctactgcag cttcgagtcc
      541 atccagaaaa gccacctgtg ccggtttcag ttgttttttg gccatgatca gtatactgtc
      601 taaggacata gcaccatgat tatgaccatc atcggggcta tgctgatcaa ctggggcggc
      661 tacagttttt ttcgccggaa ccgtctttcc aaagacatat aatacagctg tcaacacaac
      721 tgctgcagcc aaggttatcc attgaggtct cttcactcat caaaaattta gaactgcaaa
      781 agtagctcca aaatagttgt aatccctgcc aaaagcagca ttttgtattt aattaattgg
      841 gcctgcacgt catgaaaaaa tagaaaagca tatggtctga ttcgtaaatc aatgtaattc
      901 caggctaatt ctctgtccca cgattattta ctatcttaaa ctccgtttag aaaccagtta
      961 atcattttca ccataaccaa gcaacctatg cgcctgcttt tacccacact gctcttcact
     1021 ctttttttat tcattacctc ttgcggcaat aaaagcaaaa agcaaacgga acaaactgat
     1081 atttcattga taaaagacag tctttcttca aggcaggctc gggcagacac aaccctggct
     1141 gctataatgc cgataataga aaaggaactg aacgcctgga taaagtcatt taacggcttt
     1201 agtacagatt cattcaaata cacccagaca acaaagtttg aagaaatcgc ctaccaggat
     1261 acaaccggaa tgaataagtt ttatgagtta tacaaaccct cactggtttt ttcgggagac
     1321 agcagtcagt tcatagatat ctatagcgcc ggaattatgc tggaaaagaa gggaaagaaa
     1381 attgttgcca gctcagatgt ggaccaggcc attacccttt gcaacctgca aaccggtgat
     1441 tggagacaaa ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnntgg tttgaagcca gaattataag accgcaatca
     1621 gtgaattacg aagccagtgg tttagcgaaa ttgaaaatag acgagtggga ataagttatt
     1681 attctccatc ctcacccggg ccggtgtctg gcttgggctc tttcaatttt tttacttcgg
     1741 ccacaaactc cttggcaggc ttgaaagccg gtatataatg ttcggggata tgtactgcta
     1801 tgttcttctt gatgttacgt ccgatcttgg ctgctctttt cttggttata aagctgccga
     1861 aaccgcggat ataaatattt tcgccaccgg caagggtgtc ttttacttct ttgaacatag
     1921 tttcaagagt aacaagcaca tctaccttgg gtatgcctgt tttttcagaa atctgattta
     1981 ccaggtcggc ttttctcatg attgcaggtt tttagtggtt ttctgatgcc aatttatgat
     2041 aaaattagat gatagtcaag caaataggaa taattttttt caacccactg tgcattccgg
     2101 gtgttatttt gccagataat tccaaatttt agcagttttc cagaccttgc cagtgaacca
     2161 ttggtgttga acccagggcc ccaaacatga agtcaaagca aaaattttct tctcaactct
     2221 taaactggaa taaaaaccag aataaaagaa aaatgccctg gaaaggtgaa aaagaccctt
     2281 ataaaatctg gctcagcgag atcattctgc aacaaacgag ggtggagcaa gggctggctt
     2341 attacaataa tttcattaat acctttccca ctgttcatca cctggccaaa gcgccggatg
     2401 aaaaagtgtt taaattatgg gaaggccttg gttactattc ccggtgccgt aacctgctgg
     2461 ctactgcacg gtttatctcc gaagaactaa aaggaatatt cccagacagt tatgagagta
     2521 ttaaggcatt gaagggaatc gggccttata ccgccgctgc catttcctct tttgctttca
     2581 acctgccgca tgccgtggt
//
LOCUS       DAORLS010000391         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21691_length_2589_cov_1.939621, whole genome shotgun sequence.
ACCESSION   DAORLS010000391 DAORLS010000000
VERSION     DAORLS010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21691_length_2589_cov_1.939621"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..927)
                     /gene="mazG"
                     /locus_tag="PK977_18785"
     CDS             complement(151..927)
                     /gene="mazG"
                     /locus_tag="PK977_18785"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRF20235.1"
                     /translation="MSTEIEAAFSRLVNIMDDLREKCPWDKKQTIQSLRQMTIEETYE
                     LADAITENDWKGIREELGDLLLHIVFYAKIGKEQNQFELHEVINGICEKLIARHPHIY
                     GDVQVDNDEDVKKNWEKIKLKEGKQSVISGVPQSLPATVKAMRLQEKAKQVGFEWDNK
                     EQVWEKVEEEIGELKIAVASGDHGKSEEEMGDVIFSLINYARFLQVDAENALERTNKK
                     FIHRFTTMEKQALQTGKSLNEMTLQEMDAIWNTIKQQRSE"
     gene            complement(940..2016)
                     /locus_tag="PK977_18790"
     CDS             complement(940..2016)
                     /locus_tag="PK977_18790"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRF20236.1"
                     /translation="MSHTPQRKEKDCLNCGTIVQGKYCQNCGQENIVPHETFWHMVKH
                     FLYDITHFDSKFFDSMKYLLLRPGFLPQEYIKGRRASYLNPVKKYVFTSAIFFLLFFS
                     FFLKKDTISINVSGPVSSDDKVLIKKFVEDELAKDTGNMKWKDALIRINDTATVLKYS
                     DVASFWSKTTTMRFGNKIYRGLAHYDSVQKILPSKEKDGWLRRIGIRKILQVNEKYKD
                     DPRTAFNKLRDSVFHKLPYLLFVSLPLFALILKLLYFRHKDLYYVDHGIFSIYHYIFT
                     FILLLFVFAMDKLNNLVGWSFLETVMGLVFLSGGFYLYKSMRKFYKQKRAKTILKFLL
                     LNIAGLVMMAFLFSLFVLFSVFEI"
     assembly_gap    2192..2371
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2372..>2589
                     /locus_tag="PK977_18795"
     CDS             <2372..>2589
                     /locus_tag="PK977_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20237.1"
                     /translation="TKTVTYSRFDKQGRIISIADSSTAVDNTTSYEYDTKGNIIKVQN
                     TVTDSASQFDHTEIHLWYYDEVGKPARM"
BASE COUNT          730 a          522 c          422 g          735 t
ORIGIN      
        1 caagtaactt tcagcctttt taccttcctg tgttactgaa gatctgctgc tgtaaatttt
       61 agtaaagaaa aaagaaaaga ataacagaaa agccgacagg atcagcaagc tgtactttgt
      121 cccggggata ttttttaaat gtggcttcac ctactcggac cgctgttgtt tgatggtatt
      181 ccagatggca tccatttcct gcaatgtcat ttcattaagg cttttaccag tttgcagggc
      241 ttgtttttcc atcgtggtaa accggtggat gaatttttta ttcgttcttt ctaatgcgtt
      301 ttccgcatca acctgcaaaa accgtgcata attaatcagt gaaaatataa catcgcccat
      361 ctcctcctca gatttaccat gatcaccgct ggcaacagct attttcaact ctcctatttc
      421 ctcttccact ttttcccaca cctgttcttt attgtcccat tcaaagccta cctgtttggc
      481 cttttcctgc aaccgcatgg ccttaaccgt ggctggcaag gattggggta ccccgcttat
      541 aacagattgc tttccttcct tgagctttat cttttcccag ttttttttca catcttcatc
      601 attatccacc tgcacatcac cataaatatg cggatggcgg gcaattagct tctcacaaat
      661 accattgatt acttcatgga gttcaaactg gttttgttct ttaccaatct ttgcataaaa
      721 tacaatatgc agtaaaaggt cgcccaattc ctcacggata cccttccagt cgttttccgt
      781 aattgcatct gccagttcat aggtctcctc aatggtcatt tggcgcaagg actgaatagt
      841 ttgcttttta tcccaggggc acttttcccg aaggtcatcc ataatattta ctaagcggga
      901 aaaggcggct tcaatttcag ttgacatgta aagaatgaat taaatttcaa aaacagaaaa
      961 cagtacaaat aaagaaaaca ggaatgccat catcaccagg cctgcaatat tcagcagcag
     1021 aaacttaaga atggtcttgg cccttttttg tttataaaat tttcgcatgg atttgtacag
     1081 gtaaaaccca cctgataaga aaactaaacc cattactgtt tcaaggaaag accagccgac
     1141 caggttattc agcttatcca ttgcaaacac aaacagcaat aaaatgaaag taaaaatata
     1201 gtgatagata ctaaaaatac catgatccac ataatagaga tccttatgcc tgaaataaag
     1261 cagctttagt attaaggcaa agagtggcaa tgaaacaaac aacaagtacg gaagcttatg
     1321 gaaaacagaa tcccggagct tgttaaatgc agtgcgggga tcgtctttat acttttcatt
     1381 tacctgcaaa atttttctta taccaattcg tctcaaccat ccgtcttttt cttttgaagg
     1441 taatatcttc tgaaccgaat cgtaatgagc taatcccctg taaattttat taccaaatcg
     1501 catggtcgta gtttttgacc aaaatgaagc cacatccgaa tactttaaca cagtagccgt
     1561 atcattgatc cggatcaggg catccttcca tttcatatta ccggtatctt ttgccagttc
     1621 atcctcaaca aactttttta taagtacttt atcatcagaa gagaccggac cactaacatt
     1681 gatagatatg gtatcttttt tcagaaagaa gctgaaaaaa agcaggaaaa agatagccga
     1741 ggtaaaaaca tattttttta cagggttcag gtagctggcc cttcttccct ttatatattc
     1801 ctggggcaaa aatcccggcc tcaggagtaa gtacttcatc gaatcaaaaa atttactgtc
     1861 gaagtgggta atatcataca ggaaatgctt gaccatgtgc cagaaggttt cgtgcggtac
     1921 aatattttcc tggccgcaat tctggcaata tttaccctga acaatggtgc cacagttcag
     1981 gcaatccttt tctttacgtt gcggagtgtg cgacacggta aaattttctt aaaaataagt
     2041 acaaataaaa tacaagggct gccagttttt ccgttactga acaattctgg taactttgat
     2101 gattaataca atatcgccga tatgaaattc ctgctgacag tttgtttttt atccgctgca
     2161 tcctttctca acgctcagta ctattataat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngactaaaac cgttacatac agccgttttg
     2401 acaaacaggg caggattatt agcattgccg attcctctac tgctgtagac aacacgacca
     2461 gctatgaata tgatacaaaa ggaaatatca tcaaagttca aaacactgtt accgactctg
     2521 caagtcagtt tgatcataca gaaatacatc tatggtatta tgacgaagta gggaaacctg
     2581 ccaggatgt
//
LOCUS       DAORLS010000392         2577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_21814_length_2577_cov_1.894132, whole genome shotgun sequence.
ACCESSION   DAORLS010000392 DAORLS010000000
VERSION     DAORLS010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21814_length_2577_cov_1.894132"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PK977_18800"
     CDS             <1..1263
                     /locus_tag="PK977_18800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF20238.1"
                     /translation="VPLTVDFIDTVGNAQTYEWDFGDGSPIVTTTTPNTSHVYTFTGT
                     FRVRLIAIDPNTCNIRDTSYMNIRVGDLRATLAFNPVKLPPCDSFKYRFDNLSVAPPV
                     RPFGPQSFIWDFGDGSPRITAGPVPVFHDYAGPGTYTVRLILPDTNYCNAPDSIEFIL
                     SVAENVDARFTTPATGCTPYNAAFTNTSLAGQTWFWDFGDGNTSTAFEPTHLYAAPGT
                     YTITLIAFNPNTCNLTDTARFTIQVFDGPTPDFSFAPVVPPPNTPTTFTNLSSPDAIR
                     FKWNFGDGDSLITTSRAPVLHQYNATGTFNACLTAYNAIGCENTICREVRAEIIPLVD
                     VPNAFTPLSGDINSVVYVKGFGIAKMQFTIWNRWGQKVFETNSTSQGWDGRVKGAVQP
                     MDVYVYTLNIEFFDGTKTTKKGDITLIR"
     gene            1282..2319
                     /locus_tag="PK977_18805"
     CDS             1282..2319
                     /locus_tag="PK977_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF20239.1"
                     /translation="MKSRITILLTILGFCNGATSFAQDLHFSQFMNSPLLTNPANTGF
                     MPDGDYRLGANYRNQWASVTAFPYKTMSVFGDVQTMQNNDNTGWLGLGGVILRDVAGT
                     SVLTSTKIYGSVAYHQMINAGSLLSLGFNVGWANKNINTSNLSFPSQWNGQFFDSHQT
                     GAAPKFDDNNINYLDMQVGMNYAYFPNERAYFNAGFSAMHVNRPKETFFSSDEGVDNR
                     VPVRYTGFLNGSFKLNDMLIVNPNIYASLQAKSYEIVGGLNAHYNASGDGEKVLIAGL
                     YYRYQDAFIPMVGLGFKDYTFTFTYDATTSSLKNFNGARGALEFSLIKTGVVDRYNGN
                     KRESMCPSFRY"
     gene            2388..>2577
                     /locus_tag="PK977_18810"
     CDS             2388..>2577
                     /locus_tag="PK977_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20240.1"
                     /translation="METAAYALLTAREIGTFKNIVGEDYVFMDDESLDIYAHDETENL
                     HYRPEVVLKPRTAEEISAI"
BASE COUNT          712 a          526 c          564 g          775 t
ORIGIN      
        1 gtgccactta cagttgattt tattgatacg gtaggtaatg cacaaacata tgaatgggat
       61 tttggcgatg gttcaccaat cgttacaaca acaactccca atacttctca tgtatacact
      121 tttaccggca ctttcagggt tcgcctgatt gctattgatc caaacacttg caatatccgt
      181 gatacatctt acatgaatat cagggtgggt gacctcaggg ctacattggc ttttaacccg
      241 gtcaagttgc caccttgtga ttcattcaaa taccggtttg ataatttatc agtggcgcca
      301 ccagtgcggc cattcggacc ccagtctttc atctgggatt ttggagacgg ctcaccaaga
      361 attacagctg gtcctgtacc ggtgtttcat gattatgccg gtccgggaac ttatacagtg
      421 agactaatac tgcctgatac caattactgt aatgcaccgg actcaattga atttatcctg
      481 agtgttgccg aaaatgtaga tgcccggttt actactccgg ctacaggttg tacgccttat
      541 aacgccgcct ttacaaatac ttcgctggcg ggtcaaacat ggttctggga tttcggcgat
      601 ggaaatacat caactgcgtt tgagcctacc catttgtatg ctgcgccagg aacctataca
      661 ataacattga tcgctttcaa tcccaatacc tgcaatctta cggatacggc caggtttacg
      721 atacaggttt ttgatgggcc gacacctgat ttcagttttg cacccgtggt tccgcctcct
      781 aacacaccca ctacttttac caatctgtcc tcaccggatg ccattcgttt taagtggaat
      841 ttcggtgatg gcgattcgct gatcactact tcaagagcac ctgttctgca ccagtataat
      901 gctacaggta cattcaatgc atgccttaca gcttacaatg cgataggctg tgagaatact
      961 atctgtcgtg aagtaagagc tgaaataata ccattggtgg atgtacccaa tgctttcaca
     1021 ccattaagcg gagatataaa cagtgttgtc tatgtaaaag gcttcggcat tgccaaaatg
     1081 cagtttacga tctggaaccg ctggggccaa aaagtatttg aaacaaacag cacttcacaa
     1141 ggttgggacg gaagggtaaa aggagctgtt cagcccatgg atgtttatgt ctataccctg
     1201 aatattgaat tttttgacgg aacaaaaaca actaaaaagg gtgatataac gttgatacgt
     1261 taggttacat caaatgtgaa tatgaaaagc aggattacta tattgttgac aattttaggt
     1321 ttttgtaacg gtgcaacatc atttgcacag gatctgcatt tttctcagtt catgaattct
     1381 cctttactga ctaaccccgc taataccggt tttatgccgg atggtgatta taggctgggg
     1441 gctaactatc gtaatcaatg ggcatcagtg actgcatttc cttataagac aatgagtgtt
     1501 tttggcgatg tacagaccat gcaaaacaac gacaatacag gctggttagg attaggagga
     1561 gtgatattga gggatgtggc aggaaccagt gtgctcacct ccactaaaat ttacggttcc
     1621 gttgcctacc accagatgat taatgccgga agtttgttga gtctgggatt taatgtaggc
     1681 tgggctaata aaaacattaa tactagtaac cttagttttc ccagtcaatg gaatggccag
     1741 ttttttgact ctcatcaaac aggtgctgct ccaaaatttg atgacaataa tatcaactac
     1801 ctggatatgc aggtagggat gaattatgct tatttcccta atgaaagggc ttattttaat
     1861 gcgggttttt ctgccatgca tgtcaaccgt ccaaaagaaa cattttttag ttcggatgaa
     1921 ggagtggata acagggtgcc tgttcgttac accgggttcc tgaacggtag tttcaaactg
     1981 aatgatatgc tgattgtaaa tccgaatatt tatgcttctt tacaggcaaa atcttatgag
     2041 atagtaggcg ggctaaatgc ccattacaac gcatcgggtg atggagagaa agtgttgatt
     2101 gccggactct attaccgcta ccaggatgcg ttcattccta tggttgggtt gggctttaaa
     2161 gattatacct tcaccttcac ctacgacgct actacctctt cattaaagaa ctttaatggt
     2221 gccaggggtg ctcttgaatt ttcccttatt aaaaccgggg tggttgatcg ctataatggc
     2281 aataaaaggg aatctatgtg tccttctttc cgctactgac ctttctttcc cgtttgactg
     2341 attattccga tttttacagc cttaataagt aataatagct gacatacatg gaaactgctg
     2401 cgtatgcttt attgacagcc cgggaaatag gtacattcaa aaatattgtg ggtgaggatt
     2461 atgttttcat ggatgatgaa tcattggata tttatgctca tgatgaaaca gaaaatttgc
     2521 attatcggcc ggaggttgtc ttaaagccca gaacagcaga agaaataagt gctattt
//
LOCUS       DAORLS010000393         2553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22077_length_2553_cov_1.578863, whole genome shotgun sequence.
ACCESSION   DAORLS010000393 DAORLS010000000
VERSION     DAORLS010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22077_length_2553_cov_1.578863"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..827
                     /locus_tag="PK977_18815"
     CDS             276..827
                     /locus_tag="PK977_18815"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20241.1"
                     /translation="MKYFFSLLLLHFSIALFAQRKIDVLHYKYEIDLNDNNDTIKGRA
                     TITFLTVESTLNITLDLAERGIKKKGMSITSVTFPSRNALNPLVEHKNSKLSLTPVKG
                     FNKGDTATVIINYEGIPSDGLIISKNQFGKRTFFGDNWPNRAHNWIPCVDDPSDKASV
                     EFIVTAPSHYQVISNGIQLEETN"
     gene            878..>1413
                     /locus_tag="PK977_18820"
     CDS             878..>1413
                     /locus_tag="PK977_18820"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF20242.1"
                     /translation="MADFAVQHTGFVNNCIPVSSWVYTENKKEGFYDYTIGKDILQWF
                     INYIGPYAYPKLANVQSSTMFGGMENAGAIFYADNTVNGQRTEERLFAHEIVHQWFGN
                     MATEKSFAHFWLSEGFATYLTHVYLESKYGTDSLNKRMQEDRDQVLAFVKYAKRPVVD
                     STTDYMRLLNANSYQKGS"
     assembly_gap    1414..1481
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1482..1857
                     /locus_tag="PK977_18825"
     CDS             <1482..1857
                     /locus_tag="PK977_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20243.1"
                     /translation="AQYAGKNAETKDLQKVFEINSGKKLDNFFQQWLNRPVNPQLEIK
                     WKYLPGNQKIELTVSQLQDSAPFELPLELSLDMGKARPKFEKLYISKKVETFTLNTGT
                     GLVTIKPDPNISLLFEAKVIRQ"
     gene            1916..>2322
                     /locus_tag="PK977_18830"
     CDS             1916..>2322
                     /locus_tag="PK977_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRF20244.1"
                     /translation="MLIKTFGSAVYGVEAITITIEVNWIATQGKDPMIVGLPDNAVKE
                     SLQRIESTFKTNGYSFPRTKVVVNLAPADIKKSGTAFDLPIALGILGASEQIEKAEVL
                     SQYVIMGELALDGEIRPIKGALPIAIQARKEGF"
     assembly_gap    2323..2334
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2335..>2553
                     /locus_tag="PK977_18835"
     CDS             <2335..>2553
                     /locus_tag="PK977_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF20245.1"
                     /translation="IVPKVNAKEAGMVNNLHVYGVSHIKEVIDFFENEEKGLEPVVVN
                     TREEFFNSQYDFEIDFADVKGQENIKRA"
BASE COUNT          810 a          480 c          527 g          656 t
ORIGIN      
        1 gagataacat tgtttaccag cactggacaa acattgcaga caatggaaaa gcccgtttac
       61 ccgggtggaa attttattaa cctggatgta gagaaatatc ccaaagctgt ttatttatta
      121 agggtccgtc atctggccag cggcaaaacc aaaacattaa aacttctaaa ttaaaaacca
      181 gctaaatagt tcctaaaaac cctgtaaagc ctctttgcag ggtttttagt tttttgtttt
      241 gaaacaatca ggtaactttt gcaaaattta aacgggtgaa atattttttt tcattgctct
      301 tattgcattt cagcatcgca ttatttgctc aacggaagat tgatgtactg cattataaat
      361 atgagatcga tctgaatgac aacaatgata ccatcaaagg cagggcaaca ataacctttc
      421 ttacagttga atccactttg aatattacgc ttgatttggc agaaagaggc attaagaaaa
      481 aaggcatgag tattacttcc gttactttcc cctcccgtaa tgctttaaat cccctggttg
      541 agcacaaaaa cagcaaactg tcattaaccc ctgtaaaagg tttcaataaa ggagatactg
      601 ccacagtaat tatcaattac gaaggtattc cttcggatgg cctgatcatt tccaaaaacc
      661 agtttggcaa acgaactttt tttggcgaca actggcccaa ccgtgcccat aactggattc
      721 cttgtgtgga tgatccatcg gataaagcat cggttgagtt tattgtaaca gccccctccc
      781 actaccaggt gatatcaaac ggcatacaat tagaagaaac aaattaaccc attggaaaga
      841 agatgttccc ctgccaacca aagtaatggt gataggtgtg gctgattttg cagtacagca
      901 tacagggttt gtgaataatt gtatccccgt cagcagctgg gtatatacag agaataagaa
      961 agaaggcttt tatgattata ccatcggcaa agacattctg caatggttca tcaattatat
     1021 cggcccttat gcttatccca aactggccaa tgtgcaatcc agcactatgt ttggcggtat
     1081 ggaaaatgcc ggagctatct tttatgcaga caataccgtt aacgggcaga gaaccgaaga
     1141 aagattattt gcacatgaga tcgtacatca gtggtttggc aatatggcca ctgaaaaaag
     1201 ctttgcccac ttctggctga gtgaaggttt tgcaacctat ctcactcatg tttacctgga
     1261 atcaaaatat ggaacagata gtttgaataa gcggatgcag gaagacaggg accaggtatt
     1321 ggcatttgta aaatatgcca aacgtccggt ggtggacagc accactgact atatgagact
     1381 gctgaatgca aacagttacc agaaaggcag ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgcacaata tgcaggcaag
     1501 aatgctgaaa caaaagacct gcaaaaagta tttgaaataa attccggaaa aaagctggat
     1561 aattttttcc agcaatggct gaaccggccg gtaaacccgc aattagaaat aaagtggaaa
     1621 tacctgcccg gcaaccagaa aattgaactg acagtgagcc agctacagga ttccgctccg
     1681 tttgagttac cgctggaatt atcattagac atgggaaagg caaggcctaa gtttgaaaaa
     1741 ctgtatatct caaagaaggt tgaaacattt actttgaata caggaaccgg actagttact
     1801 attaaaccag accccaatat ttctttgcta tttgaggcaa aagtgatcag acaataagag
     1861 aagaatacca ctttccggta ataggctgat tggcaatttt tgctatcttc cctccatgct
     1921 tatcaaaaca tttggcagtg cggtttatgg tgtagaagcg atcaccatca ccattgaagt
     1981 gaactggatc gccacgcagg gaaaagaccc gatgattgtt gggctgccgg ataatgccgt
     2041 gaaagaaagt ttgcagcgaa ttgaaagcac attcaaaaca aatggttatt catttccccg
     2101 caccaaagtg gtggtgaacc tggccccggc tgatatcaaa aaaagcggaa cagcattcga
     2161 cctgcccatt gccttaggca tacttggtgc ctctgagcaa atcgaaaaag cagaagtgct
     2221 ttcgcaatat gtcatcatgg gtgaattagc actggatggt gagatccgtc ccattaaagg
     2281 agcactgcct attgcgattc aggccaggaa agaaggattt aannnnnnnn nnnngatcgt
     2341 tcccaaagta aatgctaaag aggccggcat ggtcaataat cttcatgtat atggagtaag
     2401 tcatatcaaa gaagtgattg atttttttga gaatgaagaa aaaggactgg aacctgtagt
     2461 cgttaataca agagaagagt tttttaattc tcaatatgat tttgaaattg atttcgctga
     2521 tgtaaaggga caagaaaata ttaaaagggc att
//
LOCUS       DAORLS010000394         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22118_length_2549_cov_1.999599, whole genome shotgun sequence.
ACCESSION   DAORLS010000394 DAORLS010000000
VERSION     DAORLS010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22118_length_2549_cov_1.999599"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PK977_18840"
     CDS             complement(<1..1144)
                     /locus_tag="PK977_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20246.1"
                     /translation="MKNCSGPLPAPHHLTLLFCFFFVFLLTLNGFSQPEKLQQLKAWE
                     REIITAYKNKDFESIIRIGDSMLVLAPANKRVLGSCFDIALNQLKDTTKALSYLQKAV
                     EQHPYDEYFLSNYGWLLLLRKQFTKAVIACTSAYQANRSSVPAIINYAHCLQLMGLTK
                     DAAALYEEAMALTTSLDSYLQMQDADYAFLHSLFPSAGFDSVRIAEREKMNAALTQYT
                     RANKLYNEFEEKNVKGKTTIKEIIPLLNAGLEEEKKQSPFRAKRISKYLTTLGNTIYN
                     TGEVKTAFKKYEEAISYNFYLKDYMELGKIYFNIGVIYKNIRSLDSAFYYFDGSAYYY
                     SRAGNRLEEAGAYDAMANVLTINGETERALPIVRDKVIPAMEKYNAY"
     gene            1288..>1991
                     /locus_tag="PK977_18845"
     CDS             1288..>1991
                     /locus_tag="PK977_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20247.1"
                     /translation="MKLPVVLIFSSFLFISSVTIAQPVPTAEEEAFFTKLMSGINSKH
                     VQWVKNTARETNEKKLSPEDIKAKTQAYAVLGSMNGQDIEALAFLVMMQAAKSAQEDL
                     KAIMAKVKAINVQKAKQRELLSKMQQQRTMTAIQLDSFKLLQNRTLALQQGRNADSIK
                     FVRSSGRNQQVSKADIDAMKDKLKSDLDSMSEMGEMESLRLQMAMDRMSKMMSTLSNL
                     LKKISKTADDIIQNLK"
     assembly_gap    1992..2014
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2231..>2549
                     /locus_tag="PK977_18850"
     CDS             2231..>2549
                     /locus_tag="PK977_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRF20248.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIANDEGRRTTPSVVAFLK
                     NGERKVGDPAKRQAITNPHNTIASVKRFMGRRFDEVGEEITHWSYKVVKGDNNTVRID
                     IDGR"
BASE COUNT          772 a          555 c          519 g          680 t
ORIGIN      
        1 catacgcatt gtatttttcc atagcaggaa ttactttatc cctgacaatg ggcagtgccc
       61 tttctgtttc accattaatt gttagcacat tggccattgc atcataggcg ccggcttcct
      121 ctaatcggtt cccggcccgg gaataataat aggccgaacc atcaaaataa taaaacgctg
      181 aatccagtga gcggatattc ttataaatca caccaatatt gaaatatatt tttcccagtt
      241 ccatataatc tttgagataa aaattatagg aaatagcttc ttcgtatttt ttgaaagctg
      301 tttttacttc tccggtattg tagatggtat tgcctaaagt agtgagatat ttactgattc
      361 gctttgcccg gaagggagat tgtttttttt cttcttccag cccggcattg agcagcggta
      421 ttatttcttt gatcgttgtt ttccccttta cattcttttc ttcaaattca ttgtatagtt
      481 tgttggccct ggtatattgc gtaagggctg cattcatttt ttctctttcg gcaatccgta
      541 ccgaatcaaa accggcggac ggaaacaaac tgtgaaggaa agcataatcg gcgtcctgca
      601 tttgcaagta ggagtcaagg ctggttgtta aggccatggc ttcttcatat agtgctgcgg
      661 catcttttgt aagtcccatt agctgcaggc aatgtgcata gttgatgatg gcaggcacag
      721 aggaacgatt ggcctggtag gcactcgtac aagcaataac ggctttggta aactgctttc
      781 tcagcaatag taaccagccg taattactca agaagtattc atcataagga tgttgttcaa
      841 cagctttctg caggtagctc agtgctttgg ttgtatcttt taactggttc aaggcaatat
      901 caaaacaaga acccagtacc cgtttgttag ccggcgcaag cactaacatt gaatccccga
      961 tccggatgat gctctcaaaa tctttgtttt tataagcggt tattatttct ctttcccatg
     1021 cttttaactg ttgtagtttt tctggttgtg aaaatccgtt taatgttaat agaaagacaa
     1081 agaaaaaaca aaacagtagg gtgaggtgat gaggtgcagg gagaggaccc gaacaatttt
     1141 tcatgatccg gaaattttaa aaaaagatct gaaactgaat gtaagttaaa gcaaatcatt
     1201 taagtgtaac gtctttttag caagaacaaa attgattttc agtatgaaca gggaaaagca
     1261 gaactttagt agctaaactt atttactatg aaacttccgg tagtacttat attcagcagt
     1321 ttccttttca tttcatctgt aacaatagcc cagccagtcc caacggctga agaggaagcc
     1381 ttttttacca aactgatgtc gggtatcaac tccaaacatg tgcagtgggt aaaaaataca
     1441 gccagggaaa caaatgagaa aaaactatct ccggaagata tcaaagcgaa gacgcaagca
     1501 tatgctgttc taggttctat gaacggacag gatattgaag cccttgcttt tcttgttatg
     1561 atgcaggcag caaaatccgc acaggaagac ctgaaagcaa ttatggccaa agtgaaagca
     1621 attaacgtac aaaaagcaaa acagcgggaa ctgctttcaa aaatgcagca gcagcgaact
     1681 atgaccgcta ttcaattgga ttcttttaaa cttcttcaaa acaggaccct tgccctgcaa
     1741 cagggacgta acgctgattc aatcaaattc gtccgttctt ccggccgaaa ccaacaggtt
     1801 tccaaagcag atatagatgc catgaaggat aaactcaaat cagacctcga ctcgatgagc
     1861 gaaatgggag agatggagag cctccgcctg cagatggcca tggaccggat gagcaaaatg
     1921 atgagtaccc tctccaacct cctgaaaaag attagtaaaa cggcggacga tatcatccaa
     1981 aacttaaagt annnnnnnnn nnnnnnnnnn nnnnaagtaa aatccatcgc aatacaccca
     2041 atccccccat taaccaatcc ccggttaacc caataaccca tttaaccaat tcccagttaa
     2101 ccaattagcc agttcccaat tcccaattcc caattaacca attccccacc caccagcatg
     2161 acaaaatttc actttccgcc cccgcggcac aatgattgac aagctatagc gaactcaaaa
     2221 atctagaatc atgggaaaaa tcataggaat agacttagga accaccaata gctgcgtttc
     2281 cgttatggaa ggcaacgaac cggtggtgat tgccaacgac gagggacgcc gtacaacccc
     2341 gtctgtagtg gcttttctga agaatggcga acgcaaagtg ggtgaccctg ccaaacgcca
     2401 ggccatcacc aacccgcata ataccatcgc ttccgtgaag cgttttatgg gccgccgttt
     2461 cgacgaagtg ggcgaagaga tcacccactg gagctataaa gtggtaaaag gtgacaataa
     2521 taccgttcgt attgacatag atggcaggc
//
LOCUS       DAORLS010000395         2547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22138_length_2547_cov_1.979535, whole genome shotgun sequence.
ACCESSION   DAORLS010000395 DAORLS010000000
VERSION     DAORLS010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22138_length_2547_cov_1.979535"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1659)
                     /locus_tag="PK977_18855"
     CDS             complement(<1..>1659)
                     /locus_tag="PK977_18855"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRF20249.1"
                     /translation="EKINFSVILRDAQWNNPGYIPLKLKFLLPNGKELKSFRKSLNEE
                     GSLEGSIEIAMAAITGSYTLEVYTTTDVLLASKNFSVEEFVPDRIRVMAKLDKKSIRP
                     GENAALSINAMNFFGPPAANRKYETEIQVKQKSFSPEKFEGYDFSLANQNTFFDKEVK
                     EGMTDEAGNATIEYEVPATYANMGALQTNFFTTVFDETGRPVSRASSVDIFTQDVFFG
                     IKQDWFYYYPLNQPVKFQLAAVNKDGNTTNAMARVEVIKHEYRTVLTKSGSYFRYESQ
                     KEDKLMIEQQINVGANTFYSYVPRTPGDYEVRIYVPGANNYVQRSFYSYGSWGVNNTS
                     FEVSREGNVEIEIDKKSYTTGETVKALFKTPFSGKMLVTMETDHVVSHQYVDVSNRTA
                     SLDLKLSAEHVPNVYITATLIKPHEVSDIPLTVAHGFRNVTVEEKKRKINVEVVAQKV
                     VRSKTHQKVTVKAVPGSFVTLSAVDNGVLQISDFKTPDPYDYFYQKKALQVSAFDLYP
                     LLFAEVRARLSSTGGDGYTTDLEKRANPMPAKRVKVVSYWGGIKK"
     assembly_gap    1660..1694
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1695..>2305)
                     /locus_tag="PK977_18860"
     CDS             complement(<1695..>2305)
                     /locus_tag="PK977_18860"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20250.1"
                     /translation="GYYPKESSSGPDYASYEDEYYYDDYGSDAMLGDVIYEKEIDTRS
                     LPKSGVGRLLNFSQFEDRLPDFKGVYHVVIRSTDDYWVKDSRYISLSDLGLIAKEGLN
                     KIYVFANSIKTADPVNGVNVAVYGSNNQLIGTGATSSSGVAEIAYTKKDFGGFKPAMI
                     IAKTADDFNYLPFSNTKVNTSRFDVGGKRSNPSGFDAFVYAER"
     assembly_gap    2306..2329
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2330..>2547)
                     /locus_tag="PK977_18865"
     CDS             complement(<2330..>2547)
                     /locus_tag="PK977_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20251.1"
                     /translation="LRGKLGGVMKEEYNGSVAFGELEANINFTNSKAVYLSKKGGKNI
                     EVQITNVPKVKLIISKIYENNLLLANRYG"
BASE COUNT          669 a          591 c          442 g          786 t
ORIGIN      
        1 ttttttgata ccaccccagt aactcacaac cttaactctt ttagctggca tgggattagc
       61 tcttttttca aggtctgttg tgtaaccatc cccaccggtg ctgcttagcc tggcccttac
      121 ttctgcaaac aataaaggat aaaggtcaaa ggctgatacc tgcaaagcct ttttctggta
      181 gaaataatcg taaggatcag gtgttttaaa atcactgatc tgcagtacac cattatctac
      241 tgctgaaaga gtaacaaaac tgcctgggac agctttcact gtcaccttct ggtgtgtttt
      301 ggatcggact actttctgtg ctactacttc cacgttgatt tttcttttct tttcctctac
      361 agttacattc ctgaaaccat gtgcgactgt cagtggaatg tccgatactt catgtggttt
      421 tattaaagtg gcagtaatgt aaacattcgg cacatgctct gcacttagct tcagatcaag
      481 actagctgtt cgattactta catccacata ctgatgtgat accacatggt ctgtttccat
      541 tgtcaccagc atcttgccgc taaaaggtgt cttgaacaaa gctttcaccg tttcaccggt
      601 ggtataagac ttcttatcaa tctctatctc cacattgcct tcccgactaa cctcaaacga
      661 tgtattattt actccccagc ttccgtagct gtaaaaactc ctttgcacat agttattggc
      721 gcctggaaca tatattctca cttcatagtc accgggggta cggggcacat aagaataaaa
      781 ggtgttagca cctacattga tctgttgctc aatcatcagt ttatcttcct tttgagattc
      841 atagcgaaaa taactgccgc ttttagtgag taccgtccgg tattcatgtt tgatcacttc
      901 cactcttgcc atagcattgg tggtattacc gtctttattc acggcagcca gttgaaattt
      961 cactggctga ttgagcggat aatagtaaaa ccagtcttgt tttattccaa agaatacatc
     1021 ctgtgtaaaa atatcaacgg aagaggccct gctcacgggc cgtccggttt catcaaaaac
     1081 agtggtaaaa aaattcgttt gcagtgcgcc catgttggca taagtagccg gcacttcgta
     1141 ttcaatcgta gcattacctg cttcatcagt catgccctct ttcacttctt tatcaaaaaa
     1201 agtattctgg ttagccaggc taaaatcgta accttcaaat ttctctggag aaaaagattt
     1261 ttgcttcacc tgtatttctg tttcatactt tctgttagca gccgggggac caaagaaatt
     1321 catggcattg atagaaagag cagcattttc ccccgggcga attgattttt tatcaagctt
     1381 ggccataact ctgatcctgt caggaacaaa ttcttctacc gaaaaattct ttgaagccag
     1441 caacacatct gtagtagtat aaacctccag cgtataactg ccggtaatag ctgccattgc
     1501 gatttcaata cttccttcca gtgagccttc ttcattcagg ctcttcctga atgatttcag
     1561 ttctttccca ttaggcagaa ggaatttcag cttcaacggt atatacccgg gattattcca
     1621 ttgtgcatca cgaaggataa ctgaaaagtt gatcttttcn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnntctttc tgcataaaca aatgcatcaa aacctgaagg attacttcgc
     1741 tttccaccaa catcaaagcg ggatgtattt actttcgtat tactaaatgg caggtagtta
     1801 aaatcgtctg ctgtttttgc tataatcatt gccggtttaa agcccccgaa atcttttttt
     1861 gtataagcaa tctctgccac gccgctgcta ctggtggccc cggtgcctat caactgattg
     1921 tttgagccat atacggccac attcacgcca ttaaccgggt cagccgtttt aatagaattg
     1981 gcaaagacat aaatcttatt caatccctcc tttgctatca gaccaagatc agataaagaa
     2041 atatagcggc tgtcttttac ccagtaatca tcggtggaac gtataaccac atgataaact
     2101 cctttgaagt caggtaatct gtcctcgaac tgggaaaaat tcaacaatct tcctaccccg
     2161 cttttgggca acgaacgggt atcgatctct ttctcataga tcacatctcc aagcatggca
     2221 tcactgccat agtcatcata ataatattca tcctcataac ttgcatagtc gggtccactg
     2281 ctgctttctt tggggtaata accatnnnnn nnnnnnnnnn nnnnnnnnnc cataccggtt
     2341 cgccaaaaga aggttattct catagatttt cgaaataatc agcttcacct ttggtacgtt
     2401 ggtgatctgc acttctatat tctttccacc ttttttagag agataaactg ctttgctatt
     2461 agtgaaattg atattagcct ctaattcgcc aaaagcaaca ctcccattat actcttcttt
     2521 cataacgcca cccaacttac cacgaag
//
LOCUS       DAORLS010000396         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22434_length_2519_cov_1.975244, whole genome shotgun sequence.
ACCESSION   DAORLS010000396 DAORLS010000000
VERSION     DAORLS010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22434_length_2519_cov_1.975244"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="PK977_18870"
     CDS             <1..1528
                     /locus_tag="PK977_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRF20252.1"
                     /translation="GDFDACYGGWSVEGETFTKEPGTDWMWWRARMLGGRTNHWGRIS
                     LRFGPKDFKRKSIDGLGDDWPIGYEDVKPYYDKVDKLIGIFGTNEGLENDPDGFFLPP
                     PKPRLHELFIKKAALQAGVNVIPSRLSILTKQIENDAGRGVCFYCGQCARNCSMAKAD
                     FSSTNVLIYPALKTGNIDVVANAMVREVLTNKDGLATGVSYISKEDMMEYQVEGKVVI
                     LAASACESARILLNSKSQRHPNGLANSSNVVGKYLHDSTGAALGGVLPEMFGRKRYNE
                     DGVGGLHVYSPWWLDNKKLDFPRGYHIEYWGGMSQPSYGFSWGIEKLNGKYLVNGKQK
                     EAGGYGASLKEDYRFFYGAGVGMAGRGEAIPLETNYCEIDPNTVDKYGIPVLKFNVKW
                     SEHEIKQAKHMKETFKEIMHNMGAVITWGGDDNEKNNWGLSKPGEIIHEAGTVRMGND
                     PKKSALNKWSQAHDCKNLFCVDGGQFVSQADKNITWTILALSMRASEYIIDEMKKQNL
                     "
     gene            1606..2277
                     /locus_tag="PK977_18875"
     CDS             1606..2277
                     /locus_tag="PK977_18875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HRF20253.1"
                     /translation="MDRRKSLKLIATGVIAAPAVIAGCKNDDKQEVGNKPAENKFNLD
                     RNPDELKYENELLAKEKFFTEHEMATITILADIIIPKDEVSGSAADAKVPEFIEFIVK
                     DIPQHQVPMRGGLRWLDMQCLRRYEKAFKDCSVQQQIEMVDQIAYPATAKPEMSQGVQ
                     FFSLMRNLTASGFYTSEIGVKDVGYIGNSPSQWNGVPEDVLKQYNLSYTEKELKECVS
                     FDNNK"
     gene            2325..>2519
                     /locus_tag="PK977_18880"
     CDS             2325..>2519
                     /locus_tag="PK977_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20254.1"
                     /translation="MNRQLVSTIFFLFSALALAAQNNYVTLHEDCNYGGKRFFLEAGT
                     YREYQMRIDNDKLSSMQIPAG"
BASE COUNT          804 a          466 c          605 g          644 t
ORIGIN      
        1 tggtgatttt gatgcctgtt atggcggctg gtctgtagaa ggagaaactt ttacaaaaga
       61 accgggtacc gactggatgt ggtggcgggc aagaatgctt ggaggacgca ctaatcactg
      121 gggaagaatt tccctgaggt tcggtccaaa agattttaaa cgaaaaagta ttgacggcct
      181 gggtgatgat tggccaattg gttatgaaga tgtaaaacca tactacgata aagtagataa
      241 acttattggc atttttggca ctaatgaagg attagaaaat gatccggatg gtttttttct
      301 tccgccaccc aaaccaaggc tgcatgaatt attcatcaaa aaagcagcat tgcaggccgg
      361 ggtgaacgta attccttccc ggctttcaat actaacgaaa caaattgaaa acgatgcagg
      421 aaggggtgtt tgtttttact gtggccaatg tgcccgcaac tgcagtatgg ctaaagctga
      481 tttttcttcc acgaatgttc tcatttaccc tgccttgaaa accggcaata ttgatgttgt
      541 tgccaatgca atggtaagag aagtgctgac gaataaagac ggactggcta caggagtttc
      601 atatatcagc aaagaagata tgatggaata ccaggtagaa ggcaaagtgg tgatattggc
      661 agccagtgcc tgtgaaagtg caagaatatt attgaattca aaatcgcaga gacatccgaa
      721 tggtttagcc aacagcagta atgtagtagg taaatacctt catgattcta caggtgctgc
      781 attaggcggg gtgttgccgg aaatgtttgg aagaaaacgt tataatgaag atggcgttgg
      841 tggtttacac gtttattcac cctggtggct cgacaacaaa aaattagatt tcccaagagg
      901 ttaccatatc gaatattggg gaggcatgag ccagccttct tacggattca gttggggtat
      961 tgaaaagctg aacggcaaat atcttgtaaa cggtaagcaa aaagaagccg gtggatatgg
     1021 ggcatcgtta aaagaagact atcgcttttt ctatggagca ggtgtaggca tggccggccg
     1081 tggtgaagcc attccgctgg aaacaaacta ttgtgagata gatcccaata cggtcgataa
     1141 atatggcata cccgttttaa agtttaatgt taagtggagt gagcatgaga tcaaacaggc
     1201 caaacacatg aaggaaacct tcaaggaaat catgcacaat atgggagctg tcattacctg
     1261 gggtggtgat gataatgaaa aaaataactg gggcctatcc aagcccggtg agatcattca
     1321 tgaagcagga actgtacgaa tgggcaatga cccaaaaaaa tcggccctca acaaatggag
     1381 ccaggcgcat gattgtaaaa atcttttctg tgtggatggc ggacagtttg tatctcaggc
     1441 agataaaaat atcacatgga ctatccttgc attaagtatg cgggccagtg agtatataat
     1501 tgatgaaatg aaaaaacaga atttgtaaac ccctgatcat agttcatggc tcatggcaaa
     1561 cccaaatgca atgtgccata aaccatgagc caaaatttct atatcatgga tcgaagaaaa
     1621 tcgctaaaac tgattgctac cggagttatt gctgctcctg cagttattgc aggatgtaag
     1681 aatgatgaca agcaggaagt cggcaacaaa ccggcagaaa acaaattcaa tctcgacagg
     1741 aatcctgatg aactgaagta tgagaatgaa ttgctggcaa aagaaaaatt cttcaccgaa
     1801 catgaaatgg ccactattac cattttggca gatatcatca tccctaaaga tgaagtgagc
     1861 ggcagtgctg ctgatgccaa agtaccggag ttcatcgagt ttattgtaaa agatataccg
     1921 cagcaccagg tacctatgag aggcggcctt cgctggctgg atatgcaatg cctcaggcgt
     1981 tatgaaaaag catttaaaga ttgttcagtg cagcaacaaa ttgaaatggt tgaccaaata
     2041 gcttaccccg caacagcaaa gccggagatg agccagggtg ttcaattctt cagcctgatg
     2101 cggaatctta cagcaagcgg attttatacg tcagaaatag gggtaaaaga tgttggctac
     2161 attggcaatt ctccttctca atggaacggt gtacccgagg atgtgctgaa acaatataat
     2221 ttatcctata cagaaaagga actgaaggaa tgtgtgagtt ttgacaacaa taaataagtt
     2281 tcctaactta ctgatcattc ctgtcaatca ttaaactacc ttttatgaac aggcaacttg
     2341 tttcaacaat atttttcttg ttttcggcct tagcactggc tgcacagaat aattacgtta
     2401 cactgcatga ggactgcaac tatggcggca aaagattttt cctggaggca ggcacttacc
     2461 gggaatacca gatgaggatc gacaatgaca agctttcatc tatgcaaata cctgccggt
//
LOCUS       DAORLS010000397         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22436_length_2519_cov_1.663149, whole genome shotgun sequence.
ACCESSION   DAORLS010000397 DAORLS010000000
VERSION     DAORLS010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22436_length_2519_cov_1.663149"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>980)
                     /gene="tilS"
                     /locus_tag="PK977_18885"
     CDS             complement(<1..>980)
                     /gene="tilS"
                     /locus_tag="PK977_18885"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRF20255.1"
                     /translation="GYEFTIAHCNFQLRGDESNRDEDFVKELGNKYKVEVKVQRFNTE
                     QYASENKISIQEAARALRYEWFEQLVVSPDSGVGSLVLNVILTAHHKDDNIETLLMNF
                     FRGTGLHGLTGIPVSYGYIKRPLLDFSKEELLQFAKENQLEFVEDSSNLSSKYTRNFF
                     RNEIIPAISKVYPQVKENLADNIKRFKEIENLYRQTVGELKKKICKEKGAEVYIPILQ
                     MMKYNNRALIYEIIRDYGFNEKQVDELIKLAGSESGKFIESPDLQYRIIKHRHWFIIS
                     PAQSTSAANIVIEAKDRSVGFDKGILHIEMTSNGHLSSDNQVACLDAKEI"
     assembly_gap    981..1036
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1128..1439)
                     /locus_tag="PK977_18890"
     CDS             complement(1128..1439)
                     /locus_tag="PK977_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF20256.1"
                     /translation="MDRIAKLKEFLIANPADSFVQHALALEYIKLGDDEVARRLFEEI
                     LNREPGYIGSYYHLGKLLERNDNTEEAVKVYERGMEEAKKAGDNHAFGELRGAFEELT
                     F"
     assembly_gap    1605..1699
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1700..2307
                     /locus_tag="PK977_18895"
     CDS             <1700..2307
                     /locus_tag="PK977_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HRF20257.1"
                     /translation="AIELKEADASTVIHLVTVGAADAEPIIRKALALGGDEAIRVNAD
                     SHDSYYIASQIAEVAKQGGYDLIFTGKETIDYNGSSIGGMVAELLDLPYISLATKFEL
                     AGNKATVTREIEGGEEVNEVELPAVVSCQKGMAEARIPNMRGIMAARTKPLKVMEPVS
                     VDALTSVAGFGLPPAKAGVKLVDAENVDELVRLLHEEAKAI"
BASE COUNT          655 a          534 c          469 g          710 t
ORIGIN      
        1 ttatttcttt agcatcaaga caagctacct ggttgtccga cgacaggtgt ccgttgctgg
       61 tcatttcaat atgaagaatc cctttatcaa aaccaactga tctgtctttt gcctcaatta
      121 caatattggc agccgaagta ctttgtgcag gactaatgat gaaccaatgc cggtgtttga
      181 tgatgcggta ttgtaaatcc ggggattcaa taaattttcc tgattcactg cctgccagtt
      241 taatcaactc atcaacctgc ttttcgttaa agccataatc ccggatgatt tcatagatca
      301 atgcccggtt attgtatttc atcatctgaa gaataggaat ataaacttct gccccttttt
      361 ctttgcatat cttttttttc aactccccta ctgtttgccg gtaaagattt tctatttcct
      421 taaaccgttt aatattatca gccaggtttt cctttacctg cggatatact ttgctgatgg
      481 cggggatgat ctcattacga aagaagttcc gggtgtattt ggaagaaagg ttggaagagt
      541 cttctacaaa ttcaagctga ttttctttgg caaactgcag caattcttct tttgaaaaat
      601 ccaacagtgg ccttttgata taaccgtatg aaacaggaat accggttaat ccatgcaacc
      661 cggtgccacg gaaaaaattc atcagcaggg tctcaatatt gtcatcttta tgatgagctg
      721 taagaatgac attcagcacc aaacttccta ctcccgaatc aggactcact actagttgct
      781 caaaccattc gtatcgcaaa gcccttgctg cttcctgtat ggatatttta ttttcagaag
      841 catattgttc tgtattaaac cgctgaactt ttacttccac cttgtatttg ttgcccaact
      901 cttttacaaa gtcttcatcc cggttacttt catcacctct tagctgaaaa ttgcaatggg
      961 caatagtaaa ttcatatcct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnntcct ccactgacag caagaagcag tttgtctttt gatgagaaaa
     1081 gatgctgttg tttgatacta tccttaaatc gttgaagtaa actcatgcta aaaagttagt
     1141 tcttcgaaag cccccctcaa ctcgccaaaa gcatggttgt ctccggcctt ctttgcttct
     1201 tccattcccc gttcgtaaac tttcaccgcc tcttcggtat tatcatttct ttccagcagt
     1261 tttcccaggt gataatagct gccgatgtat cccggttccc tgtttaagat ttcttcaaat
     1321 aaacggcggg ccacttcatc atcacccagc tttatgtact ccagcgcaag tgcatgctgc
     1381 acaaaactat ctgccgggtt ggctatcaaa aactctttca gcttagctat cctgtccact
     1441 gtaaaaaatc ttaattagtt atgtgatttt attaagctcc cttccctatc tttgctttag
     1501 ttgcataaac aactatcctt ctaacgatct aaacaaaaca caatgaagat cctcgtttgt
     1561 atcagtaaaa cgccggacac aacggcaaaa atagccttca cggannnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnng ggccatcgaa ctgaaagaag ctgatgcctc cactgttata
     1741 catttggtaa cagtgggtgc tgccgatgcc gaacctatta tacgcaaggc attggccctg
     1801 ggcggtgatg aagctatccg ggtgaatgct gacagccacg acagctatta tattgcttcg
     1861 caaattgctg aagtagcaaa acagggtgga tatgacctca tcttcaccgg taaagaaacc
     1921 attgattaca atggctcctc catcggcggc atggtagcag aactgcttga tcttccctac
     1981 atatcattag caaccaagtt cgagctggct ggcaacaaag caacagttac aagagaaata
     2041 gaaggcggtg aagaagtaaa tgaggtggaa ttgcctgcag tagtaagttg ccagaaagga
     2101 atggctgaag caagaattcc caatatgcgg ggtattatgg ctgcccgtac caaaccattg
     2161 aaagtaatgg agcctgtatc ggttgatgca cttacttctg ttgcaggttt tggcttacct
     2221 cctgccaaag caggcgttaa actggtggat gcagaaaatg tggatgaact ggttcgcttg
     2281 ctgcacgaag aagcaaaagc catttaaggc atattcatct taaatttcaa acatcaaact
     2341 ttaaactcat tttatgtcag ttttaatctt catagatact gccgaaggcc atgtgaaaaa
     2401 atcttcgttg gaagcaatga cctatggcgc caaagttgca gagcaactgg gaaccactgc
     2461 ggaagccgtt gtgctgggtg cagtcaatga tgacctcggt gctcttggca aatacggtg
//
LOCUS       DAORLS010000398         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22462_length_2517_cov_1.824939, whole genome shotgun sequence.
ACCESSION   DAORLS010000398 DAORLS010000000
VERSION     DAORLS010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22462_length_2517_cov_1.824939"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK977_18900"
     CDS             complement(<1..199)
                     /locus_tag="PK977_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20258.1"
                     /translation="MSHNQEYHEELKKVQDSDFTHNWVSSSGFLFYLQVACFVAFTLG
                     GCYMLYTKRYEKINVPVQESSL"
     gene            complement(250..>1197)
                     /locus_tag="PK977_18905"
     CDS             complement(250..>1197)
                     /locus_tag="PK977_18905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF20259.1"
                     /translation="VTAATYSNYEVIVADNGSTDDSLSFLQLHYPGIRTILFKENYGF
                     AKGYNEALKQVKADYYVLLNSDVEVQPGWLEPMVELLEKDRSIAACQPKILSWHNKKL
                     FEYAGAAGGWLDEYGYPFAKGRVFDICEEDKGQYDQSEPIFWASGASLFIRPAVYHAV
                     KGFDEYFFAHQEEIDLCWRIQLAGHKIYSCPASVVYHVGGGTLPKGNSLKTYLNFRNN
                     RIMLSKNLPLSKKIWVMPARNLLDGISAWKGLLSGDGGYFIAILRAQLSFIKWWLFNK
                     KKSVFPEHRNKPLLGFLKKNMAWQHFVKKKKAFSEIVIN"
     assembly_gap    1198..1252
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1378..>2155
                     /locus_tag="PK977_18910"
     CDS             1378..>2155
                     /locus_tag="PK977_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF20260.1"
                     /translation="MFRQLLNWRTALAFIAILIISGTVIYSQYLARKIARDEKLKVEQ
                     WIDASKSLINAQEASDITFAFRIVKDNQSIPIIETNEKDSITNWINLDSAKAANHPDY
                     IVKKLRQFKAQNPAIIWTDPNDSTRLNRYYYGNSRLLNEVRYYPIVQLFIVALFIIIT
                     LLSLRSSYRSVQNQVWAGMAKETAHQLGTPVSSLEGWVEMLKEDQRNEKIAHELEKDV
                     NRLRLVSDRFGKIGSTPQLEEVDLVHQINNMVDYMRKRAAG"
     assembly_gap    2156..2168
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2169..>2517
                     /locus_tag="PK977_18915"
     CDS             <2169..>2517
                     /locus_tag="PK977_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF20261.1"
                     /translation="VNDHGNKQVVAKISAPLFDWVIENLLKNSLDAMEGRGSITVDIH
                     DDKKNVHIDITDTGKGISSQNIARVFKPGFTTKKRGWGLGLSLSRRIIKQYHKGEIFV
                     KHSEIGKGTTFRIV"
BASE COUNT          729 a          507 c          521 g          692 t
ORIGIN      
        1 acaggctgct ctcctgaacc ggtacattaa tcttttcata ccgcttggtg tacagcatat
       61 aacaaccacc caatgtgaaa gctacaaaac aagctacctg caggtagaaa agaaatcctg
      121 aagaggaaac ccagttgtgg gtaaaatcgc tatcctgtac ttttttaagt tcttcgtggt
      181 attcctggtt atgactcata gtggaatttt gtgttgcaaa aataggggtt caatgcaaaa
      241 aaagcgtttt cagttaatca caatttccga aaatgccttt ttctttttta caaaatgctg
      301 ccatgccatg ttttttttca agaagcccaa taaaggttta tttctgtgct ccgggaaaac
      361 gcttttcttt ttattgaaca gccaccattt aataaatgac agctgcgccc tgagtatggc
      421 tataaagtag ccgccatcgc cagataataa tcctttccat gccgatatgc catccagcag
      481 gtttcttgcc ggcattaccc agatcttttt tgacaagggc aggtttttag aaagcattat
      541 tctgttgttc cggaaattca gatatgtctt taatgaattg ccctttggca atgtgccgcc
      601 gccaacatgg taaactactg atgcagggca ggaatagatt ttatgaccag ccagttgaat
      661 gcgccagcac aggtctattt cttcctggtg ggcaaaaaag tattcatcaa atcctttgac
      721 tgcatggtaa actgcaggcc ggataaataa agaggcgccg cttgcccaaa aaatgggttc
      781 tgactgatca tattgacctt tgtcttcttc acaaatgtca aatacccttc ctttggcaaa
      841 gggataaccg tattcatcca gccagccacc tgctgcgccg gcatattcaa acaacttttt
      901 gttatgccag gaaagtattt ttggctgaca ggctgcaatg cttctgtctt tttccagtaa
      961 ctcaaccatg ggctccagcc agccaggttg aacctccaca tccgagttca gcaatacata
     1021 atagtcagcc tttacctgtt tgagagcttc gttgtatcct ttggcaaacc cataattttc
     1081 tttgaataga atcgttctga tacctgggta atgtagctga aggaaggata aagaatcatc
     1141 cgttgagcca ttatcggcaa caatcacctc ataattagaa taggtggcag cagtaacnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaactttt
     1261 gatgatagac tcaactcgct gattatttat tcaaaaataa ggtaagtagt acagtaaacg
     1321 gggcaggttt tagttactct tttttctgat ttatattgat agctgttaaa ttcgtatatg
     1381 tttcgtcaat tattgaactg gagaacggcg ctggctttca ttgccatact gatcatcagc
     1441 ggaaccgtta tctattcgca ataccttgcc cgtaaaattg cgagggatga aaaactgaaa
     1501 gtggagcaat ggattgatgc cagtaaatcc ctcattaatg cccaggaagc atccgatatc
     1561 acttttgcat tcagaatagt taaggataat caaagtattc ccattattga aaccaacgaa
     1621 aaagacagca ttaccaactg gatcaacctt gattctgcca aagcggccaa tcacccggat
     1681 tatattgtta aaaaactccg gcagtttaaa gcacaaaatc ctgcgattat ctggacagat
     1741 cctaatgatt ccacccggct taatcgttat tactatggca attcccgttt attgaatgaa
     1801 gttcgctatt acccgatcgt tcagcttttt attgtggcat tgttcatcat catcacattg
     1861 ctttctttga gaagcagcta caggtcggtg caaaaccagg tttgggcagg tatggccaaa
     1921 gaaacagcgc atcaattagg aacacctgtt tcttcactgg aaggctgggt tgaaatgctg
     1981 aaggaggatc agcggaatga aaaaattgcc catgagctgg aaaaagatgt aaaccggctt
     2041 cggttagtgt cagaccgctt tggaaaaatt ggaagcacac ctcaactgga ggaggttgac
     2101 cttgtgcacc agatcaacaa catggtggat tatatgcgga aaagagcggc aggtannnnn
     2161 nnnnnnnncg gtcaatgatc atggtaataa acaggtggta gctaaaattt ctgctccgct
     2221 ttttgattgg gtgattgaga accttctgaa aaattcatta gatgcgatgg agggcagggg
     2281 tagtatcacc gtagatattc atgatgataa aaagaatgta cacatagata ttacagatac
     2341 cggtaaaggc atcagcagcc agaacattgc aagagtgttc aagcctggct ttaccaccaa
     2401 aaaaaggggg tgggggctgg ggcttagtct ttcccggagg atcatcaagc aatatcataa
     2461 aggcgaaatc ttcgtcaaac actcagaaat cgggaaagga accacattca gaattgt
//
LOCUS       DAORLS010000399         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22556_length_2510_cov_1.482281, whole genome shotgun sequence.
ACCESSION   DAORLS010000399 DAORLS010000000
VERSION     DAORLS010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22556_length_2510_cov_1.482281"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK977_18920"
     CDS             complement(<1..428)
                     /locus_tag="PK977_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF20262.1"
                     /translation="MKAGHKPELKPRRMRHLRTLIYSGLSVLLLFSCKKNSEDTPSPT
                     PTDPVAEVLNLPSTPFNYANQALPAYLASPPIQGQLNTPADNPITDNGATLGRVLFYD
                     KNLSVNNTISCASCHKQQNAFSDPVAKSAGALVVPQVAIGT"
     gene            521..>665
                     /locus_tag="PK977_18925"
     CDS             521..>665
                     /locus_tag="PK977_18925"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRF20263.1"
                     /translation="MINSIQDFQQPFFIGIAGSGMSAIAQYLKGIGKNVSGSDRFFAP
                     GVYN"
     assembly_gap    666..805
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <806..1904
                     /locus_tag="PK977_18930"
     CDS             <806..1904
                     /locus_tag="PK977_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF20264.1"
                     /translation="QLGIPIMKRSELLAMIAASKKTIAVGGTSGKSTTSAMLFAILEY
                     AGMQPSIISGAGLISIIKHGKVGNAKVGKGEWLVIEADESDGSIVQYKPEIGLLLNVD
                     KDHQEIDELMRIFDIFKKNSKRFIVNQSQLLAQKLSQNPQQDFSANPAIKAGYVAANF
                     RQSGMDISFEINGVPFELHLAGKHNMENALSATAVANQIGVDLAICGEALRSYEGIYR
                     RHQVLGNKHGVWLIDDYAHNPVKCAAAMEACHPIAPKLIAWFQPHGYKPTKFLREDFV
                     KEIAGVLRAQDEIWMSEIFYAGGTAVKDISAADLINDLKALGKQAFFVENRNDFLESV
                     RPHFTENCVLLLMGARDPSLEQFAKQTWGKL"
     assembly_gap    1962..2105
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2106..2420)
                     /locus_tag="PK977_18935"
     CDS             complement(<2106..2420)
                     /locus_tag="PK977_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20265.1"
                     /translation="MKWEAAESSNQHEVYQLYKDDKKILSLTVNKFSNSARVDCNKEK
                     RVFIIRKEGFLKNKTVLRNEYGIKIGELGSENRENFIDINDERFYYTIHNNPLAELVI
                     YKE"
BASE COUNT          637 a          422 c          505 g          662 t
ORIGIN      
        1 gtcccgatcg cgacttgcgg cacgacaagc gcaccggcgc tttttgccac cgggtctgaa
       61 aaggcattct gctgtttatg acacgaagca caggaaatag tattattcac ggaaagattc
      121 ttatcataaa aaagtactct tcctaaggtt gcaccattat cagtgatagg gttatcagcc
      181 ggtgtattca actgcccttg tatcggtggt gatgccaggt aagccgggag agcctggttc
      241 gcataattaa acggggttga cggaaggttc aatacttctg ctaccgggtc tgtaggggtt
      301 gggcttggtg tgtcttcgct atttttttta caggaaaaca ataatagtac ggataatcct
      361 gaatagataa gggttcttag gtggcgcatt cgtcggggct ttagttccgg cttatgaccg
      421 gctttcaaaa ttttggttta agataggtca aaatttcttt ttcaggcatg gtatcatcat
      481 acgggtttag ccggcgggaa taatttcttt tatttgcatc atgatcaatt ctatacagga
      541 ttttcagcaa ccttttttta taggaatagc cggaagtggt atgagtgcca tagcccagta
      601 tttaaaagga attggaaaaa acgtttccgg aagtgatcgc ttttttgctc ctggagttta
      661 taatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnacaac taggcatacc catcatgaag cggtctgaac
      841 tactggccat gattgcagca agtaaaaaaa caattgctgt tggtggtacc agtggaaaga
      901 gcactaccag tgccatgttg tttgctattc ttgaatatgc aggtatgcag cccagtatta
      961 tcagtggagc cgggcttatc agtattatca agcacggtaa agtagggaat gcaaaagttg
     1021 gaaaggggga gtggctggtg atagaggcgg atgaaagcga cgggtcaatt gtacaatata
     1081 aaccggagat cggtcttctg ttgaatgttg acaaagacca ccaggagata gatgaactga
     1141 tgcggatttt tgatattttt aaaaagaaca gcaaaagatt tatcgtcaat caatcccagc
     1201 tactggcaca aaagctatca caaaaccccc aacaggattt ttctgccaac ccggctatca
     1261 aagcaggtta tgtagcagcc aatttccggc agagcggaat ggatatcagc ttcgaaataa
     1321 acggagttcc ttttgaactg cacttagcgg gtaaacacaa catggaaaat gccctgtcag
     1381 caaccgctgt tgccaatcaa ataggtgttg atctcgctat ttgtggagaa gcgttgagat
     1441 cttatgaagg aatttatcgc cgccaccagg tactgggaaa taaacatggt gtatggctga
     1501 ttgatgatta tgctcataac ccggtgaaat gcgctgctgc catggaggcc tgtcacccca
     1561 ttgcacccaa actgattgcc tggtttcagc cacacggtta caaaccaacc aagtttctga
     1621 gagaagattt tgtaaaagag atagcgggag tattgagggc tcaggatgaa atatggatga
     1681 gtgaaatttt ttatgcagga ggcacagcgg tgaaggacat ttctgctgct gaccttatca
     1741 atgatttaaa agctttagga aagcaggctt tttttgttga gaacaggaat gatttcctgg
     1801 aatcagttcg gcctcatttt acagagaatt gtgttttgct gctgatgggt gccagggatc
     1861 cttcccttga acaatttgcc aaacagacct ggggaaaatt ataagatact acattgcaaa
     1921 cgctggaaca ctctctttcg taacaggaag aaacatatac cnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnttctt tatatataac cagttccgcc agcgggttat tgtgaatggt ataataaaat
     2161 cgttcatcat tgatatcaat gaaattttcg cggttctctg aacctaattc accaatcttt
     2221 atcccatatt cattacgaag caccgtttta ttttttagaa aaccttcttt acgtatgata
     2281 aaaactcttt tctctttatt acagtctacc cttgcagaat tggagaattt attgactgta
     2341 aggcttagta tttttttatc gtccttatac aactgataaa cttcgtgttg gtttgaactt
     2401 tccgcagctt cccatttcat atagttaagt gttggtttgc ttcaaaaatt acgtttcttt
     2461 tccgcttcgt aagacaagta acaaaaaatt aatattaact tcatattgga
//
LOCUS       DAORLS010000400         2502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22658_length_2502_cov_1.997139, whole genome shotgun sequence.
ACCESSION   DAORLS010000400 DAORLS010000000
VERSION     DAORLS010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22658_length_2502_cov_1.997139"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK977_18940"
     CDS             complement(<1..566)
                     /locus_tag="PK977_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRF20266.1"
                     /translation="MKLSIGFSPCPNDTFIFDALINKKIDTDGIEFEAILEDVETLNQ
                     WALEGKLDISKLSFPAFFRSTDKYLLLNAGSALGKGVGPLLVKRAGSEISKVEEQSIL
                     IPGINTTANLLLSFAYPDAKNKRPMLFSEIEDLVMSELADLGVIIHENRFTYQQKGLE
                     KVADLGESWEKKMNVPIPLGGIAIKRTV"
     gene            complement(576..1232)
                     /gene="mqnB"
                     /locus_tag="PK977_18945"
     CDS             complement(576..1232)
                     /gene="mqnB"
                     /locus_tag="PK977_18945"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRF20267.1"
                     /translation="MNCLIVAATAQEIAPFLEYYRNAKKTEHIDMHLDVLISGIGMTA
                     TTYHLVKQFSIRKPDMVVQAGVAGCFDTSLPLGTVVAIKQDTIADESVVELKNLKTLF
                     DLKLVPQNQPPYKNGWLINPANETLKRTKLKAVKGISVNQITTSKQMIRFYKEQFNPV
                     TESMEGAALHYVCLSENVPFLQIRSISNYIGERNKKKWNMADSIAGLNKELIRLLESL
                     "
     assembly_gap    1452..1467
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1468..1808
                     /locus_tag="PK977_18950"
     CDS             <1468..1808
                     /locus_tag="PK977_18950"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRF20268.1"
                     /translation="NQQNEKVFGKCALPNYHGHNYDLVVKVTGEPDPETGYVMDMKDL
                     SDIIKREVVEGFDHKNLNLDTVEFKNLNPTAENIAVVIYTILRPFIDPAKDLQIRLYE
                     TARNFVEYPA"
     gene            1837..2433
                     /gene="folE"
                     /locus_tag="PK977_18955"
     CDS             1837..2433
                     /gene="folE"
                     /locus_tag="PK977_18955"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRF20269.1"
                     /translation="MAYKKTEHYDEQVTSGLITHYKSVLNLLGEDAEREGLVKTPERL
                     AKAMQYLTQGYHLDAKAIINSAKFHEQVSEMIVVKDIEIYSMCEHHMLPFIGKAHVAY
                     IPNGWITGLSKIARVVDVYARRLQVQERLTVQIMNAIKETLNPLGVAVVIEAKHLCMM
                     MRGVQKQNSVTTTSAFDGEFQKNSTRGEFLRLITADLD"
BASE COUNT          720 a          497 c          510 g          759 t
ORIGIN      
        1 tcaactgtcc ttttgatggc aattccgcct aaaggaattg gtacattcat tttcttttcc
       61 caggattctc ccaggtctgc cactttttct aatccctttt gctgataagt aaaacggttt
      121 tcatgaatga taacacccaa atcggccagt tcactcatca ccaggtcttc aatttcagaa
      181 aagagcatgg gtcttttgtt ttttgcatcc gggtatgcaa agctgagcag caggttggct
      241 gtcgtattaa tacccggtat taaaatactt tgctcttcca ctttgcttat ttcactgcca
      301 gcccgtttta ccagcaaggg accaacgcct tttccgaggg cactgcctgc attcagtaat
      361 aaatatttat cagttgatct aaaaaatgcg gggaaactta gtttgctaat gtccagcttt
      421 ccttccaatg cccattggtt gagtgtttcc acatcttcca gtattgcctc gaattcgata
      481 ccgtctgtat caattttttt attgatcagg gcatcaaaaa taaaagtgtc gttggggcag
      541 ggcgaaaagc cgatactaag cttcatgagt taaacttata aactttctaa caggcggatc
      601 agttccttgt ttaatccggc aatggagtca gccatattcc atttcttttt atttctttca
      661 ccaatataat tggaaatgct tcttatctgt aaaaaaggaa cattttcaga caggcataca
      721 tagtgcagcg ctgcgccttc cattgattcg gttaccgggt taaactgttc tttataaaag
      781 cggatcattt gttttgatgt ggtgatctgg ttcacagaaa ttcctttgac cgctttaagc
      841 tttgttcttt ttagtgtttc attagcagga ttgatcagcc acccgttttt atagggaggc
      901 tggttttgcg gaaccaattt caggtcaaat aatgttttca ggttttttag ttccactaca
      961 ctttcatcag caatcgtatc ctgcttgata gctacaacag ttcccagtgg tagtgaagtg
     1021 tcaaagcaac ctgctacacc agcctgcacc accatatcag gctttcgaat ggaaaattgt
     1081 tttaccaggt gatatgtggt ggcagtcata ccgattccgc tgatcaatac atcgagatgc
     1141 atatcaatat gttccgtttt tttcgcattt cggtaatatt cgaggaaagg tgctatttcc
     1201 tgggccgtag cagctacaat aaggcaattc attacattat tttcagtgtg caaggtaaca
     1261 aagagaactt catcctgcta actttgaagt agctttgaat tcattggatt aatcaattaa
     1321 ccgcttatgg caacaattat ccaggttgat tgttgttaag agctaatctg aatcacacga
     1381 taaagcatga ataaacgagt ggctatatac cgaaaagagc atttcaatgc agctcaccgt
     1441 cttttcaacc cnnnnnnnnn nnnnnnnata accagcagaa tgagaaggtg tttggcaagt
     1501 gtgccctgcc taattatcat ggccataatt atgaccttgt ggtgaaagtg accggtgagc
     1561 cggatccgga gactggttat gtgatggaca tgaaagacct gagtgatatt attaaaagag
     1621 aagtggtgga aggctttgat cacaaaaacc tgaatcttga tacggttgaa tttaaaaacc
     1681 tgaacccgac tgctgaaaac atagctgttg tgatctatac aattctcaga ccatttatag
     1741 atcctgcaaa ggatttgcaa atcagacttt atgaaacagc ccggaatttt gtagagtatc
     1801 cggcctgaaa ttttttcttc aaatcaatta accgttatgg catataaaaa aacagaacac
     1861 tatgatgagc aggtaacctc cggtctcatc acccattata aatctgttct taacctttta
     1921 ggtgaagatg cagaaagaga aggtttggtg aaaacacccg aacgcctggc caaagcaatg
     1981 caatacctta cacaaggtta tcatcttgat gccaaggcta tcattaactc tgccaaattt
     2041 catgagcagg taagcgaaat gatcgtcgta aaagacattg agatatacag tatgtgcgaa
     2101 catcatatgc tcccctttat aggcaaggca catgtggctt atatacccaa tggctggata
     2161 accggcttaa gtaaaattgc aagggtagtg gatgtatatg caagacgact gcaggtgcag
     2221 gaacggttaa cggtgcagat catgaatgcc atcaaagaaa cactaaaccc cctgggtgtg
     2281 gcagttgtaa ttgaagcaaa gcatttgtgt atgatgatgc ggggtgtaca gaaacagaac
     2341 tcggtgacaa ccacttctgc ctttgatggt gaatttcaga aaaattctac ccgcggtgag
     2401 tttttaaggc ttattacagc agatctggac taactcctat atttgcgagg ttattaagtt
     2461 tgattgaaac tattcagtca aactttttta atgaggatat at
//
LOCUS       DAORLS010000401         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22763_length_2492_cov_1.679524, whole genome shotgun sequence.
ACCESSION   DAORLS010000401 DAORLS010000000
VERSION     DAORLS010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22763_length_2492_cov_1.679524"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>248)
                     /locus_tag="PK977_18960"
     CDS             complement(<1..>248)
                     /locus_tag="PK977_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20270.1"
                     /translation="IATLISFCLLAQQTRFYSDPEEKFKEAKEYFQKEQYSLAYPLFK
                     ELKLSVRETDKANVPVTVQEINYYTTVCALKQNEERAE"
     assembly_gap    249..370
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            521..>803
                     /locus_tag="PK977_18965"
     CDS             521..>803
                     /locus_tag="PK977_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF20271.1"
                     /translation="MKKLFSPKYSDNSITFSLLFLRLSMGGLMIPHGYKKLMNFAAKS
                     STFADPFHIGGPLSMSLTIFAEFFCAGLILIGLMTRLATIPLIIAMSVAL"
     assembly_gap    804..816
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            958..1518
                     /locus_tag="PK977_18970"
     CDS             958..1518
                     /locus_tag="PK977_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20272.1"
                     /translation="MKRSQLIEYGIISIGLISGYKFLENVVSVIFQLAYAFSDGLVDR
                     ISLLLPVFVLLAVYGLCFVILIRKSGQIATWLQGNTPNDHIAVRLEAKTLLYVILIGI
                     MIFSIISHIPKIVYYLYESFKNEISSKNLFTTRETSVRKDEFVLSSIQVIIAFLAIYF
                     ARNITGLFIRKNAAHELTFQSEPETK"
     gene            1521..1931
                     /locus_tag="PK977_18975"
     CDS             1521..1931
                     /locus_tag="PK977_18975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRF20273.1"
                     /translation="MFTTETQIRVRYAETDQMGVVYHSNYFPYFEAARAEAIREMGMT
                     YADMEKAGIIMPVVDVHCRYLRPARYDDLLTIKTILKELPVHHKIEFHHEVYNEQKEL
                     LAAGKIILYFMEAKSMKRTGMPVSLIDKLQPYFS"
     assembly_gap    2113..2211
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2212..>2492
                     /locus_tag="PK977_18980"
     CDS             <2212..>2492
                     /locus_tag="PK977_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20274.1"
                     /translation="NELGIFFIPLLGAYVSGSKREVSQMQSAVILLAGPLPGIVIGTI
                     MYLLAQTGQGYNFLDIGLERTGLLFIILNLINLFPVYPLDGGQLLNRVF"
BASE COUNT          629 a          446 c          462 g          721 t
ORIGIN      
        1 ctcggccctt tcttcatttt gctttaacgc acatactgtg gtgtagtaat tgatttcctg
       61 caccgtaacg ggcacattgg ctttatctgt ttctctcaca gaaagcttca gttccttgaa
      121 aagcggatag gcaaggctat attgttcttt ctgaaaatat tcttttgcct ctttgaactt
      181 ctcttccgga tctgaataaa accgggtttg ctgtgcaagt aagcaaaaac tgatgagggt
      241 ggctatacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn gtgtgaaaca gaatatgctt taacattttg ttggttatta gtctggcagt
      421 tgatccttag aagatgcagc tgtatgcgtt cattttgaca tagctaagat gaaagcaaca
      481 aaatttcttt tccttaatat tgcagcctaa aaatcaaccc atgaaaaaac tgttcagtcc
      541 taagtattcc gacaattcta ttaccttttc tttattgttt ctgcgccttt ccatgggtgg
      601 actgatgatt ccacatggct ataagaaact gatgaatttc gcagctaagt cttccacttt
      661 tgcggatccc tttcatattg gaggcccctt atccatgtca ctgacgattt ttgcagaatt
      721 tttttgtgca gggttgatac taatcggcct gatgacaagg ctggcgacaa ttccgctgat
      781 cattgccatg tctgttgccc tttnnnnnnn nnnnnnttat tctcataatg gtgaaatgtt
      841 tggtgaggga gaaaaggccg cccttttcct tgctggttac atagccttgt tgtttgccgg
      901 acccggtaaa cttagtgccg acaaactgat cgggaaatag tcattaactt cccgttcatg
      961 aaaagatcac agttaataga atatggcatt ataagcattg gacttatatc agggtacaag
     1021 ttcctggaga atgttgtatc ggtaattttt cagctggcct atgctttttc tgacggactt
     1081 gttgacagaa tcagcctgtt gctgccggtt tttgttttgc ttgcagttta cggtttatgc
     1141 ttcgtaatcc tgataagaaa aagcgggcaa attgccacct ggttacaggg aaacactcct
     1201 aatgatcata ttgcagttcg gcttgaagca aaaactttgc tttatgtgat acttattgga
     1261 ataatgattt tttcaattat tagccatatt ccgaaaatcg tttattattt atacgaatcc
     1321 tttaagaatg agattagtag taaaaatctt tttactacta gagaaacatc agtaagaaag
     1381 gacgaatttg ttctctcatc tatacaggtt attattgcat tccttgctat ctattttgct
     1441 agaaatatta ccggtttgtt cattcgtaag aatgcagcac atgaacttac ctttcaatca
     1501 gaacccgaaa caaaataata atgtttacaa cagaaacgca gataagggtc cgttatgcag
     1561 agactgatca gatgggagtg gtttatcata gtaattactt tccttacttt gaagcagccc
     1621 gggccgaagc cattcgtgaa atgggaatga cctatgcaga tatggaaaag gccggtatta
     1681 tcatgccggt tgttgatgtg cactgccgct atcttcgccc tgccaggtat gatgacctgc
     1741 tgaccattaa aacaatctta aaagaacttc cggtacatca taaaatagaa tttcatcacg
     1801 aagtttacaa tgagcaaaag gagttgctgg cagctggcaa gatcattctg tacttcatgg
     1861 aggctaaatc aatgaagcgt accggtatgc cggtttcttt aattgataag ctacaaccct
     1921 attttagcta aatttatata tgacctattc tgaggggcct atagccggga attcaaatga
     1981 cgaaaacatg ccactggaaa atcagcctgt gacagaatca gcatttgtgt atccgccaaa
     2041 atatgtgaag aaagaacaaa cagccaatat ctggcttcgg tcattggcaa gccttgctgc
     2101 ctacctgatt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacaatgagc
     2221 tggggatttt ctttattccc cttttaggcg catacgtatc cggttcaaaa agagaagtgt
     2281 cgcaaatgca atcagcagta attctgctgg cagggccgct acccggaatt gttattggta
     2341 ccataatgta tttgcttgca cagaccggac aaggctataa ttttctagat atagggcttg
     2401 aaagaacagg tttgcttttc atcattttaa acctcataaa tcttttcccg gtttacccgc
     2461 ttgacggagg acaactgctc aaccgggtat tt
//
LOCUS       DAORLS010000402         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_22826_length_2487_cov_1.576069, whole genome shotgun sequence.
ACCESSION   DAORLS010000402 DAORLS010000000
VERSION     DAORLS010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22826_length_2487_cov_1.576069"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    208..230
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <231..1176
                     /gene="prfB"
                     /locus_tag="PK977_18985"
     CDS             <231..1176
                     /gene="prfB"
                     /locus_tag="PK977_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131330388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRF20275.1"
                     /translation="SILKEIKVNEYWVNLYEQTNTAVEDFAVLFEFWKGGDATEEETK
                     DAYDKAITALDDAEFKATLNEPEDELPAVVQINSGAGGTESQDWAEMLARMYRMYGEK
                     QGWKVTEIDWQWGDGAGIKTATLQFDGPFAYGFLKAESGVHRLVRISPFDSNARRHTS
                     FVSVYVYPLVDDTIEIEIKPSDVKMETSRSGGAGGQNVNKVETKVQFTHLPTGIVVVC
                     QQDRTQLGNRELAMQMLKSRLYELELQKRNAIKDANNATKKKIEWGSQIRSYVFHPYK
                     MIKDHRTDYETGNVQPVMDGELDGFIKAYLMSEKEADV"
     gene            1235..>1796
                     /locus_tag="PK977_18990"
     CDS             1235..>1796
                     /locus_tag="PK977_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF20276.1"
                     /translation="MSTGTFSYPMPVNEPVLSYAPNSPEKKRLKEVLAELKSQEIDVP
                     MYIGSKEVRTNKKIAIHPPHETKHTLGYFHAGEEKHVHQAIEAALAAKESWENMSWEN
                     RANIFLKAADLIATKYRPYMNGTTMLGQSKNVFQAEIDAACEIIDFLRFNVHFLSEIY
                     KQQPISGPGMHNRMEYRPLEGFVLAVT"
     assembly_gap    1797..1806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1807..>2053
                     /locus_tag="PK977_18995"
     CDS             <1807..>2053
                     /locus_tag="PK977_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF20277.1"
                     /translation="VLAVTPFNFTAIGGNLPTSAAMCGNVVVWKCANTQVYSAQMFMR
                     ILKEAGLPDGVINLIFVDGPTIGRVCFNHRDFAGVHFT"
     assembly_gap    2054..2210
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2211..>2487
                     /locus_tag="PK977_19000"
     CDS             <2211..>2487
                     /locus_tag="PK977_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF20278.1"
                     /translation="LRGAFEYQGQKCSAASRAYIPSNMAEEVKKKLVAGVKSFKMGSP
                     EDFTNFINAVIDEKSFNSIKRYIDNAKKDPKAEIWAGGKCDSKEGWFI"
BASE COUNT          755 a          425 c          535 g          582 t
ORIGIN      
        1 gctacaataa ttaatatctg agatagtctc agccttaaac cttaaacctt aaaccttaaa
       61 ccttaaacct taaaccgcta tctttgcacc catgacaatc gaaaaattac aggatatgcg
      121 gtcccgcatt gccgggataa ggaggtttct ttgacgtcga taaccggcag gcaaagatcg
      181 aaaatgacaa acaactctct ctgagtcnnn nnnnnnnnnn nnnnnnnnnn atccattctt
      241 aaggaaatca aagtgaatga gtattgggtg aatttatatg agcaaaccaa taccgcagta
      301 gaagattttg cagtactctt cgaattttgg aaagggggtg atgccactga agaggaaaca
      361 aaagatgctt atgataaagc aattactgct ttggatgatg ctgaatttaa agccacattg
      421 aatgagccag aggatgaatt accggcagtg gttcaaatca attcaggagc cggaggtact
      481 gaaagccagg actgggctga aatgcttgcc cgtatgtacc gcatgtatgg ggaaaaacaa
      541 ggctggaaag ttaccgagat tgactggcag tggggtgacg gtgctggtat aaagacagcc
      601 acactgcaat ttgacggccc ctttgcgtat ggatttttaa aagcagaaag cggggtacac
      661 cgcctcgtcc gtatttcacc ttttgacagc aatgcccgaa gacatacatc ttttgtaagt
      721 gtttatgtat atccattggt cgatgatact atagaaatag aaataaaacc ttcggatgta
      781 aaaatggaaa catcccgtag tggtggcgca ggcggacaaa atgtaaataa agtagagacg
      841 aaagttcaat tcacgcattt acccacaggt attgtggtcg tttgtcaaca agacagaacg
      901 caacttggta accgggaact ggccatgcaa atgttgaaaa gccgtttgta tgagctggaa
      961 ctgcaaaaaa gaaatgcaat taaagatgcc aataatgcta caaagaaaaa aatagagtgg
     1021 ggcagtcaga taaggagcta tgtatttcat ccttataaaa tgataaaaga tcaccgaact
     1081 gattatgaaa caggaaatgt gcagccggtg atggatggag aactggatgg ctttattaaa
     1141 gcctatttga tgagtgaaaa agaagccgat gtgtgacaac aggtactaaa aaaaggtata
     1201 aacagcatat aatttcacac ttaaaatttg tcatatgagt acaggaactt tttcataccc
     1261 gatgccggtt aacgaaccgg ttttatcata tgctcccaat agtcccgaaa agaaaagact
     1321 aaaggaagta ttggctgaac ttaaaagcca ggaaatagat gttcccatgt atatcggtag
     1381 taaggaagta agaacgaata aaaaaattgc catccatcct ccgcatgaaa caaaacatac
     1441 actgggttat tttcatgcag gtgaagaaaa acatgttcac caggcaatcg aagcagcact
     1501 ggccgccaaa gaaagctggg aaaatatgag ctgggagaac agggcaaaca tttttctgaa
     1561 agcggcagac ctcattgcga ctaagtaccg tccctatatg aatggtacta ccatgctggg
     1621 gcaaagcaag aatgttttcc aggcagaaat tgatgctgcc tgtgagatta ttgatttttt
     1681 acgtttcaat gttcactttc ttagtgagat atataaacaa cagccgataa gtggtccggg
     1741 catgcacaac cgcatggaat accgcccact ggagggattt gttttagcag taacacnnnn
     1801 nnnnnntgtg cttgccgtta cgccattcaa ctttaccgct atcggcggca acctgcccac
     1861 ctctgctgct atgtgtggaa atgttgtggt atggaaatgt gccaataccc aggtttattc
     1921 cgcacagatg tttatgcgga tcctgaaaga agccggtttg ccggacggag tcatcaacct
     1981 gatttttgtg gatggcccta ctattggcag ggtttgcttt aatcacagag attttgccgg
     2041 agttcacttt actnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attgcgtggt
     2221 gcttttgaat accagggaca gaaatgttca gctgcctcaa gggcttacat tccatccaat
     2281 atggctgaag aagtaaaaaa gaaactggtt gcaggggtga agagttttaa aatgggaagc
     2341 ccggaagatt ttaccaactt catcaatgca gttattgatg aaaaaagttt caacagcatc
     2401 aagcggtata tagataatgc aaaaaaagat cctaaagcag aaatttgggc aggtggtaaa
     2461 tgtgacagta aagaaggttg gtttatt
//
LOCUS       DAORLS010000403         2450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_23252_length_2450_cov_2.138622, whole genome shotgun sequence.
ACCESSION   DAORLS010000403 DAORLS010000000
VERSION     DAORLS010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23252_length_2450_cov_2.138622"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK977_19005"
     CDS             complement(<1..267)
                     /locus_tag="PK977_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite S-adenosylmethyltransferase"
                     /protein_id="HRF20279.1"
                     /translation="MNNNDAQLKEIVRQKYSEIALQDKETNQSSCCGAGGCSTEVYNI
                     MSDDYTTLEGYNPDADLGLGCGLPTQFAKIQKGNTVIDLGSGAGN"
     gene            complement(295..630)
                     /locus_tag="PK977_19010"
     CDS             complement(295..630)
                     /locus_tag="PK977_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF20280.1"
                     /translation="MAFHKKEEFTQKEQDLAAFAKAMSHPARIAILKVLAQKNECICG
                     EIVDVLPLAQSTVSQHLKELKNAGLINGETDGPRSCYCINWKAFEKFNQEFNQLFSHL
                     KIKNEKACC"
     gene            complement(700..2040)
                     /locus_tag="PK977_19015"
     CDS             complement(700..2040)
                     /locus_tag="PK977_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRF20281.1"
                     /translation="MKKAVLFFMVACIYFSSYAQVLPLREQARVMDEMLADRINNLLP
                     QLMERNKIDMWILVSREYNEDPVLKTMLPATWLSARRRTILVFYHNPSKKVYEKLAIA
                     RYNVGDNIKAAWDMQQFPDQWDVLSDIIRTRNPQQIGINLSKHFAHADGMVHTEYTEL
                     MEKIPSEFKGRVVSAETLAVNWLETRTEREMAVYPQLVSITHQIIEEGFSEKVITPGI
                     TTTDDVVWWFRQKISNMGLETWFHPSVAVQRSDSTNFEHLRSFSNRPKDDIIQPGDLL
                     HVDIGITYLRLNTDCQQHAYVLKPGETRVPQSIRDAFLKANRLQDILTSEFEKGKTGN
                     QVLSGALARAKAEGITATIYTHPLGFHGHAAGPTIGLWDQQGGVKGSGDFPLNYNTVY
                     SIELNAASTIPEWKKSIRIMLEEDGYFDQSGFRYIGGRQKEIHLIPRPKTATGL"
     assembly_gap    2124..2140
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2141..>2450)
                     /locus_tag="PK977_19020"
     CDS             complement(<2141..>2450)
                     /locus_tag="PK977_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20282.1"
                     /translation="LLSGFLVPLVTMIIHYTSGIHPDGLFIVDFKLPSGIEWLWILLM
                     GLAALFGQYFVTRAFGADKVGIVSIFGYANIIYSVFIGMLLGDAFPDGMTWAGITLII
                     V"
BASE COUNT          656 a          561 c          494 g          722 t
ORIGIN      
        1 attaccggca ccgcttccca ggtcaattac cgtattgcct ttttgaatct ttgcaaactg
       61 tgtaggcagc ccacagccta aaccaaggtc tgcatcagga ttatatcctt ccaatgtggt
      121 gtaatcatca ctcatgatat tgtaaacctc ggtagagcag cctcctgccc cacagcagga
      181 agactggttg gtttctttat cttgcagggc tatttcgctg tatttctgcc ggacgatttc
      241 ttttaattgt gcatcgttgt tattcatggt taaactattt atcaggttaa aaatttaaca
      301 gcaggctttt tcattcttta ttttcaggtg gctgaaaagc tggttgaact cctggttgaa
      361 tttctcgaat gctttccagt tgatgcagta gcaacttctg ggtccatctg tttcaccatt
      421 gatcaatccg gcattcttta attctttcag gtgttgtgat acggtgcttt gcgccaatgg
      481 taatacatcg acaatttcgc cgcaaataca ctcatttttt tgagccagta ctttgaggat
      541 agcgatcctt gcaggatgac tcattgcctt ggcaaaggcc gccaggtcct gctctttttg
      601 ggtaaattct tctttcttgt gaaaagccat aatttaatct tatatcgcaa atatacgatt
      661 aatgtgatat aagtcacaag ctttttttga aatatttttt tacagccctg ttgcagtctt
      721 tggacggggg atgagatgga tctccttttg acggccaccg atataacgaa atcctgactg
      781 atcaaaataa ccatcctctt cgagcataat acgaatggat ttcttccact ctggaatagt
      841 ggatgcggca ttcaattcga tagaatacac cgtattataa ttaagcggaa aatcgccact
      901 gccctttacc cctccctgct gatcccacaa cccaatggtg gggcccgctg catggccgtg
      961 aaaaccgaga ggatgtgtat atatagtggc cgttattcct tctgccttcg ccctggccag
     1021 cgcacccgac aacacctggt taccggtttt tcctttttcg aattctgacg ttaaaatatc
     1081 ctgaagcctg tttgctttga ggaatgcatc acggatactt tgcggaaccc gggtttctcc
     1141 gggctttaaa acataagcat gctgctggca atctgtattc agccggagat aagtgatccc
     1201 gatatctaca tgcagcaggt cgccgggctg aatgatatca tcctttggcc ggttggagaa
     1261 actgcgaagg tgctcaaaat tggtgctgtc gcttcgttga acggcaacag acggatgaaa
     1321 ccaggtttcc aatcccatat tggaaatctt ttgccgaaac caccaaacca catcatccgt
     1381 ggtagtgata ccgggagtaa tcactttttc tgaaaatcct tcttcgataa tctggtgtgt
     1441 gatggacacc agttgaggat aaaccgccat ttctctttct gttcttgttt caagccagtt
     1501 tactgccagc gtttccgcag aaacaaccct gcctttgaac tccgatggaa ttttctccat
     1561 gagttcagtg tattctgtgt ggaccattcc gtctgcatgt gcaaaatgct ttgacaggtt
     1621 aatgcctatc tgttgcgggt tcctggtgcg aatgatatcc gacaggacat cccactgatc
     1681 aggaaattgc tgcatatccc atgcagcttt aatattatct cctacattat accgggctat
     1741 tgccagtttc tcatatacct ttttcgatgg attgtgataa aatacaagga tcgttcttct
     1801 gcgggcggat aaccaggtag ccggcagcat ggtttttaga accgggtctt cattgtattc
     1861 ccggctgacc agtatccaca tgtctatttt atttctttcc atcagctgcg gcaataaatt
     1921 attgatcctg tcagccagca tttcatccat tacccttgcc tgctcccgaa gcggtaacac
     1981 ctgtgcataa gaagaaaaat aaatacatgc taccatgaaa aacaacacag cttttttcat
     2041 atgaactatt tagttccgaa aataaaaaac aataatcagc gggatatttt ttgtttcctt
     2101 tttactaaag tgataataac accnnnnnnn nnnnnnnnnn actataatta aagtaatgcc
     2161 tgcccaggtc attccatccg gaaaagcatc gccgagcaac ataccaatga aaacggaata
     2221 aataatattg gcataaccaa aaatagaaac aatgcccacc ttatcagccc caaaagccct
     2281 tgttacaaag tactggccaa ataaggcagc aaggcccatc agtaaaatcc aaagccattc
     2341 aatgccggag ggaagtttga aatcgacaat gaaaagacca tccggatgaa tgccgctagt
     2401 ataatgaatg atcatggtga ccaggggaac tagaaagccg ctgagtaaaa
//
LOCUS       DAORLS010000404         2411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_23746_length_2411_cov_1.685908, whole genome shotgun sequence.
ACCESSION   DAORLS010000404 DAORLS010000000
VERSION     DAORLS010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23746_length_2411_cov_1.685908"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PK977_19025"
     CDS             <1..808
                     /locus_tag="PK977_19025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF20283.1"
                     /translation="TATNAAGCSQTDSVRIEVRSVNNFTVNPPVEVCQNSTIQLSASG
                     GDVYNWTPASTLDNPSLANPIASPVASTPYFVTITDTLCNNSSTLSTTVTVLPLPVVR
                     ANKSNDIDCSFPQSRLTATGATQYVWTPAASLNNPSSPTPVAFPAVTTQYVVQGTDAD
                     GCTNTDSVKVIVSATNKGGYLMPTAFTPNNDGLNDCYGIKYWGIIQELEFSIYNRWGE
                     RIFFTRNPGECWDGRYKGIPQDPAVFIYMIKAKTTCENNIFRKGTFTLIR"
     gene            complement(801..1304)
                     /locus_tag="PK977_19030"
     CDS             complement(801..1304)
                     /locus_tag="PK977_19030"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF20284.1"
                     /translation="MIVFETERLIVRHFRKSDADDFFLLQGDPDVMRFIRPVKNREES
                     DAFLFEAIAGYTEPWMGRWAIEEKEAGRFVGSFVVNPIPGDNVKTQLGYAFIPDCWGK
                     GFATEVTRGGLTYFNLVTPLQEIYGVVESENLASQKVLLKTGFQPFDKFMEGEKEILR
                     FVFKRST"
     gene            complement(1308..>2021)
                     /gene="prfC"
                     /locus_tag="PK977_19035"
     CDS             complement(1308..>2021)
                     /gene="prfC"
                     /locus_tag="PK977_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627548.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRF20285.1"
                     /translation="KMTGFVFKIHANLDPKHRDRIAFFRVCSGRFERNKYFHHVRLDK
                     DVRFSNPYSFMARDKSIIDDAYAGDVVGLFDTGNFKIGDTLTEGEEFFFTGIPSFSPE
                     IFKEVVNKDPMKTKQLEKGLLQLTDEGVAQLFTQFGGNKKIIGCVGELQFEVIQYRLL
                     HEYGASVVFNTLPFYKACWLTAKDPGKLEDFLRFKQANAGEDKDGHPVYLAQSEWFLN
                     TEKSNNPDIEFHFTSEIHK"
     assembly_gap    2022..2130
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2131..>2411)
                     /locus_tag="PK977_19040"
     CDS             complement(<2131..>2411)
                     /locus_tag="PK977_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20286.1"
                     /translation="TWPINSGKDFKGVYNLDQRSLLLFTANTKADEDSISISDITDKI
                     LDEKLGEKDAAQLREDVELVDGVHGQLKTDEYLAGKVAHDLVHQLAHHI"
BASE COUNT          651 a          557 c          417 g          677 t
ORIGIN      
        1 caccgcaacc aatgcagcag gttgttctca aactgactcg gtcaggattg aagtaagatc
       61 tgtaaataat tttacggtta atccaccggt agaagtatgt cagaatagta caatacaact
      121 atcggcatcc ggcggggatg tatataactg gactcctgct tcaacgctgg ataacccatc
      181 actcgctaat cccattgctt caccagtagc ctcaacacct tattttgtaa ctataactga
      241 tacactttgc aataactcct ctaccctctc tacaaccgtt acagttttac ccttacctgt
      301 tgtcagagcc aataaatcga atgacattga ttgttcgttt ccgcaaagcc ggttaaccgc
      361 taccggagcc actcaatatg tctggacacc tgctgcctca ttaaacaacc catcatcccc
      421 aacacccgtt gcttttcctg ctgtgactac acaatatgtt gttcagggaa ctgacgctga
      481 tggttgtaca aatactgata gtgtgaaggt aatcgtttct gccactaaca aaggcggata
      541 cctgatgccg actgcattta cgcctaataa tgacggacta aatgactgct atggtattaa
      601 gtactggggt atcattcagg aacttgaatt cagcatttac aaccgctggg gcgaaagaat
      661 attttttaca aggaatccgg gagaatgctg ggatggcagg tataagggta taccacaaga
      721 ccccgccgtt tttatctata tgattaaagc caaaacaacc tgtgaaaata atattttccg
      781 aaaaggaaca tttacactta tcaggtagat cttttgaata cgaatcttaa tatttccttt
      841 tcaccttcca taaacttgtc aaatggctga aaccctgtct taaggagcac tttttgtgaa
      901 gcaagattct cagactccac taccccatat atttcctgta aaggtgtaac caagttaaaa
      961 taagtcaggc cccctcttgt aacttcagtt gcaaaacctt tcccccagca atcaggaata
     1021 aaagcatagc ccaattgtgt tttaacatta tccccgggga tcgggtttac aacaaatgat
     1081 ccgacaaacc tgcctgcctc tttttcctcg atcgcccatc tccccatcca gggttcagta
     1141 taaccggcaa tagcttcaaa cagaaaggca tcactttctt ccctattttt taccggccgt
     1201 ataaaacgca ttacatcagg atcgccctgc aacaaaaaga aatcatccgc atcagacttc
     1261 ctaaaatgcc ggacgattaa tctttctgtt tcaaaaacga tcattgctta tttgtggatt
     1321 tcagacgtaa aatgaaattc aatatcgggg ttattggatt tttctgtatt caagaaccat
     1381 tcactttgtg ccaggtaaac agggtgtccg tctttgtcct ctcctgcatt ggcctgttta
     1441 aaacgcaaaa agtcttccag tttcccggga tctttggctg taagccagca agccttgtaa
     1501 aaaggcagtg tattaaaaac taccgaagca ccgtattcat gcaaaagccg gtattgtatc
     1561 acctcaaatt gaagctcccc cacacagcca ataatttttt tattcccgcc aaattgtgta
     1621 aaaagctgtg ccaccccttc atccgttaac tgcaggaggc ctttttccaa ctgcttggtc
     1681 ttcattgggt ctttattcac cacttcttta aatatttcag gcgaaaaaga cggaatgccg
     1741 gtaaaaaaga attcttctcc ttctgtcagg gtatctccga ttttaaaatt tcccgtatca
     1801 aataatccta ccacatcccc ggcataggca tcatctatga tgcttttgtc tctagccata
     1861 aatgagtagg ggttgctgaa acggacatct ttgtccagcc gcacatgatg aaagtattta
     1921 ttgcgttcaa atctacctga acaaaccctg aagaatgcta tccggtccct gtgcttggga
     1981 tcgaggttgg catgtatttt aaatacaaag ccggtcattt tnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caatgtgatg ggcgagctga tgaaccagat
     2161 cgtgggcgac tttaccggca aggtattcat cagtttttaa ttgaccgtgt accccatcca
     2221 ccaattccac atcctccctt agttgagctg catctttttc acccagcttt tcatccagta
     2281 ttttatcagt tatatctgaa atggaaatac tgtcttcatc agccttggta ttggcggtga
     2341 ataacaataa acttctctgg tcaagattgt aaactccttt aaaatccttc ccgctgttaa
     2401 tcggccatgt c
//
LOCUS       DAORLS010000405         2405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_23813_length_2405_cov_1.676596, whole genome shotgun sequence.
ACCESSION   DAORLS010000405 DAORLS010000000
VERSION     DAORLS010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23813_length_2405_cov_1.676596"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK977_19045"
     CDS             complement(<1..218)
                     /locus_tag="PK977_19045"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRF20287.1"
                     /translation="MLKRYLNIAEYDIESSFVIGDRITDVQLAKNLGCKAIWINNDAS
                     LGAAEITNDITSLQSTIALETNNWKSIY"
     assembly_gap    267..456
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(539..1591)
                     /gene="hisC"
                     /locus_tag="PK977_19050"
     CDS             complement(539..1591)
                     /gene="hisC"
                     /locus_tag="PK977_19050"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRF20288.1"
                     /translation="MKFELDKLVRENIKKMTPYSSARHEYAGEAAIFLDANENSFGSP
                     LQQNYNRYPDPLQQKLKEKISSIKGVPVQNIFLGNGSDEAIDLLFRIFCNPGKDNVII
                     LPPTYGMYEVCAEVNDVEVKKVPLLPNFQLNLEAIEQSIDSNTKIIFVCSPNNPTGNS
                     INRNDIEILLNNVNGLVIIDEAYINYARQKSFIPELIEYPNLVILQTLSKAWGLAGLR
                     LGMAFAGQPVIDYLNKVKYPYNVNTATQQLAFEALNNISSVNNWTKTTVEQREWLISQ
                     LTKLAFVNKIYPSDANFILVKIQNSKNIYDYLTGKGIIVRDRSKVILCDECLRITIGT
                     PDENKQLIDALKTYSV"
     gene            complement(1594..>2405)
                     /gene="hisD"
                     /locus_tag="PK977_19055"
     CDS             complement(1594..>2405)
                     /gene="hisD"
                     /locus_tag="PK977_19055"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRF20289.1"
                     /translation="PSKDGGINPAILFTANLCGVTKIYKVGGVQAIAAMTYGTASIPK
                     VFKIFGPGNQYVTAAKQLVQQHGVSIDMPAGPSEVLVIADETAIPEFAASDLLSQAEH
                     GPDSQVILVTTSESMAEKVNEAVNDQIKSLPRMEIASKALQNSRVIITKSIDEAIALS
                     NLYAPEHLILSCDKAEEVSYKVTNAGSVFLGNYSPESAGDYASGTNHTLPTNGFATMY
                     SGVSLDSFVKKISFQYLTKQGLQNISDTVKIMAVAEGLDAHKNAVAIRLNN"
BASE COUNT          600 a          436 c          428 g          751 t
ORIGIN      
        1 tcatagatcg atttccaatt atttgtttcc agtgcaatcg ttgattgcag gctggtaatg
       61 tcatttgtga tttcagctgc tcctagactt gcatcgttgt tgatccaaat agctttgcaa
      121 cctaaatttt tagctaactg aacatcagta atgcggtcgc caataacaaa tgaactttca
      181 atatcatatt ctgctatatt gagataacgt ttcagcatac cagtggctgg gttccttgtc
      241 ggagcattat ctgcagcaaa tgttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaatt tttcaaaagt atccacctgg
      481 taggtgggag gtgcttcttt tattagagta ccatccctgt caataaaaag tatctttctc
      541 atacgctgta tgttttcaat gcgtcaatca attgtttgtt ttcatcaggg gtgccaattg
      601 ttattctaag gcattcatca caaagaatta cttttgaacg atctctgacg ataatgcctt
      661 taccggtaag ataatcatag atgttttttg agttctgtat cttcaccagt ataaaattgg
      721 catcagaagg atatatttta tttacaaatg caagttttgt aagctgacta attaaccact
      781 ctctttgttc aaccgtagtc tttgtccagt tatttacact tgagatatta tttaacgcct
      841 caaaggccag ttgttgtgta gccgtgttga cattgtaggg atatttaact ttgttcaggt
      901 aatcgattac cggctgaccg gcaaatgcca tacctaaacg caaacctgct aatccccatg
      961 cttttgataa agtctgcagt ataacaagat tcgggtactc aattaattca ggaatgaagc
     1021 ttttctgacg ggcataatta atataagctt cgtctataat gactaatcca ttaacattat
     1081 tcagtaatat ttcaatgtca tttctgttta tactattacc cgtaggattg ttgggagagc
     1141 agacaaagat gattttagta tttgaatcta tcgattgttc aatagcttca aggttgagtt
     1201 gaaaattcgg caacaatggt actttcttaa cctccacatc gtttacttca gcacaaactt
     1261 catacatgcc ataagtgggg ggtagaatga tgacattatc cttgccggga ttgcagaaga
     1321 ttctgaaaag taaatcaatt gcctcgtcgc tgccattgcc taagaaaata ttctgaacag
     1381 gaacgccttt aatactgctg atcttttctt tcagcttttg ttgaagcgga tcgggatacc
     1441 ggttatagtt ttgttgtaac ggtgatccaa aagaattttc attagcatca aggaaaatag
     1501 cggcttctcc tgcatactca tgccgtgcag aagaataagg tgtcattttt ttaatattct
     1561 ctctaactag cttgtccagt tcaaatttca ttgttaatta tttagtcgga tggcaactgc
     1621 gtttttatgt gcatcaaggc cttctgctac tgccataatt ttaaccgtat cactaatatt
     1681 ttgtaaacct tgttttgtca aatactgaaa actgatcttt tttacaaagc tatccagcga
     1741 aactccgctg tacattgttg caaaaccatt ggtcggtaaa gtatgattgg tgccacttgc
     1801 atagtcaccg gcgctttccg gtgaatagtt gcccaggaaa actgaaccag cattagtgac
     1861 cttgtacgat acttcttctg ccttatcaca acttaagatc aggtgttcag gcgcatagag
     1921 gttagataat gcgatagctt catcaatgct ttttgtaata ataacccggc tgttttgtag
     1981 tgcttttgag gcaatttcca ttcttggcag gctcttaatt tgatcattga cagcttcatt
     2041 tactttttct gccattgatt cgcttgttgt cacaagtatt acttgcgaat cggggccgtg
     2101 ttctgcctgc gaaagcaggt cggatgctgc gaactcagga attgctgttt catcagcaat
     2161 aaccaatact tcagatgggc ctgctggcat gtcaatactt actccgtgtt gctgcaccag
     2221 ttgtttggca gccgtcacat attggttacc cggaccgaaa attttgaaaa cctttggaat
     2281 tgatgctgta ccatacgtca tggcagcaat cgcttgtaca ccgccaacct tatatatttt
     2341 tgttacaccg caaagatttg ctgtaaatag gatagcaggg ttaatgccac cgtcttttga
     2401 aggcg
//
LOCUS       DAORLS010000406         2350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_24476_length_2350_cov_2.250109, whole genome shotgun sequence.
ACCESSION   DAORLS010000406 DAORLS010000000
VERSION     DAORLS010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24476_length_2350_cov_2.250109"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>399
                     /locus_tag="PK977_19060"
     CDS             <1..>399
                     /locus_tag="PK977_19060"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HRF20290.1"
                     /translation="FSLVSNDVSKDRLVIMPGYWFMHNMYALARNAGKYQSRDKRTDK
                     TQTLEYDYLAPDSVNELFDAISLLEFSTGKAWSSQQKKAIPEKEWRKKGKQLLENEPQ
                     VVNKLEILAEGFENSKRKTVLIKTSEACQL"
     assembly_gap    400..461
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <462..990
                     /locus_tag="PK977_19065"
     CDS             <462..990
                     /locus_tag="PK977_19065"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HRF20291.1"
                     /translation="LISFIEKKKIKSWAQLQKSLPANATRENWKNAGGQLIPEPAIKN
                     LVRSIQTGKISSWDEVHAFYQAQGDKYPAEKFKHAFASLLEALHLTSSRFTKKVFLQL
                     LKQAITTKDWMTTGIYQSRAKDYNNAFRQMLYETEAEMEKVIGKLKDNSFIQQQQQEM
                     AAFTKQVETLQQLFA"
     gene            987..1373
                     /locus_tag="PK977_19070"
     CDS             987..1373
                     /locus_tag="PK977_19070"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF20292.1"
                     /translation="MNRKVDDFIEALADEQRELCELIREMIWENVPAIDERLSFKIPF
                     YHYFGMFMYINPGKQGIDIGFCRGKDLLEAFPQLTIGNRAIVAILTIRSKKDIYTKDL
                     QQLIITAAAWNEEAKRLKLPMVKKKK"
     gene            complement(1378..>2350)
                     /locus_tag="PK977_19075"
     CDS             complement(1378..>2350)
                     /locus_tag="PK977_19075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20293.1"
                     /translation="GKGSIYAPIGISGTGGVVTDQFTAEYLRTNPQSIHGACPAACAG
                     GLDHVSFVEYWKLNQDVGTSAKQVSLEVHALSFCKMLANTFVSRWNGTQWTNEATSIL
                     AGPNPCGSGLQCGTIQASNTTTVFGDFTLSTDQPFLNNPLPVTLVDLKIKKYSEQAAI
                     AEWELTDYCFTATQFELEHSTDKTSFAVIAVQPGKENSRFYFHNDTRIAKGTNWYRLK
                     ITEVDGKVTYSKIIAIINDTKGLLITSVFPNPVSNTATITLSAARSGMADVQLYDISG
                     TVVYRRRSAIAEGTNNLPIEMGKLSTGVYHLAVQSADCKAVYRLVKQ"
BASE COUNT          709 a          529 c          495 g          555 t
ORIGIN      
        1 ccttttccct ggtgagtaat gatgtaagca aggaccgtct cgtcatcatg cccggctact
       61 ggttcatgca caatatgtat gcactggccc gcaatgccgg caaataccag agccgcgata
      121 aaagaacaga caaaacacaa accctagagt acgactacct ggcacccgac agtgtcaatg
      181 aactcttcga tgccatcagc ctgttggaat tcagcaccgg caaagcatgg agcagccagc
      241 agaaaaaagc aataccggaa aaagaatggc gcaaaaaagg aaaacagttg ctggaaaatg
      301 aaccacaggt ggtgaataag ttggaaatac tggctgaagg tttcgaaaac agcaaacgca
      361 aaacggtttt gataaaaact tcggaagcct gccagctttn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nactcatcag tttcattgaa
      481 aaaaagaaga tcaaaagctg ggcacaattg caaaaatcac tgccggctaa tgcaacaaga
      541 gaaaactgga aaaatgcagg tggccaattg atacctgaac ccgccattaa aaacctggta
      601 cgttctatac aaaccggaaa gatcagcagc tgggatgaag tgcatgcctt ttaccaggca
      661 cagggcgata aataccctgc tgaaaaattc aaacatgctt ttgcatcatt gctcgaagcc
      721 ctgcacctca cttcctcccg ttttactaaa aaagttttcc tgcaactgct gaaacaggcc
      781 atcaccacaa aggactggat gaccaccggt atttaccagt caagggccaa ggactacaac
      841 aatgccttcc gccaaatgct gtatgaaacc gaagcggaaa tggaaaaagt gatcggtaaa
      901 ctgaaagaca acagctttat tcaacagcaa cagcaggaaa tggctgcatt taccaaacag
      961 gtggaaacgt tgcaacagtt atttgcatga acaggaaagt agatgacttc attgaagccc
     1021 tggccgatga gcaacgggaa ctatgcgaac tgatcaggga aatgatctgg gagaatgtac
     1081 cggccataga tgaaagacta agttttaaaa tccccttcta tcattacttc ggcatgttca
     1141 tgtatatcaa tcccggtaaa cagggaattg atatcggctt ttgccgcggc aaagacctgc
     1201 tggaagcctt tccgcagctc actattggca acagggccat tgtggctatt ctgaccatcc
     1261 gcagtaaaaa agatatttat acaaaagacc tgcagcaact catcatcact gctgccgcct
     1321 ggaatgaaga agccaagagg ctgaagctgc cgatggtgaa aaagaaaaaa taaacggcta
     1381 ctgctttacc aacctgtaca ctgccttaca gtctgccgat tgcaccgcca aatgataaac
     1441 ccctgtagaa agtttgccca tctcaatggg taaattattg gtgccttcgg caatggcgct
     1501 gcgccggcga tacaccaccg ttccggaaat atcatacaat tggacatccg ccataccact
     1561 gcgggctgca cttaaagtaa tggtggctgt attgctgaca ggatttggaa ataccgaagt
     1621 gatcagcagg cctttggtat cgttgataat ggcaattatt ttgctgtaag taaccttgcc
     1681 atccacttct gttattttca atcggtacca gttagtgcct tttgcaattc tggtatcatt
     1741 atggaaataa aaccggctgt tttcttttcc gggttgtaca gcaatgacgg caaaagatgt
     1801 tttatccgtt gaatgttcca gttcaaactg ggtggcagta aagcaatagt cggttaactc
     1861 ccattctgca atagcagcct gttcactgta ctttttaatt tttaaatcaa ccagtgtcac
     1921 cggtaatggg ttgttaagga atggctgatc agttgaaaga gtaaaatcac caaagacggt
     1981 tgttgtgtta ctggcctgaa tagtaccaca ctgtaaacca gaaccacaag gattcggtcc
     2041 ggctaatatt gatgttgctt catttgtcca ttgtgtacca ttccatcggc tgacaaaagt
     2101 attggcgagc attttacaaa aactcaatgc atgtacttcc aatgagactt gtttagcaga
     2161 agtaccaaca tcctggttca atttccaata ctccacaaaa ctgacatgat ccaatcctcc
     2221 tgcgcaagca gcaggacatg cgccgtgtat gctttgcgga tttgttcgca ggtattccgc
     2281 tgtaaattga tcggtaacaa ctccaccagt tcctgaaatc ccaatcggtg cataaatact
     2341 ccccttgcca
//
LOCUS       DAORLS010000407         2340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_24643_length_2340_cov_1.659956, whole genome shotgun sequence.
ACCESSION   DAORLS010000407 DAORLS010000000
VERSION     DAORLS010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24643_length_2340_cov_1.659956"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK977_19080"
     CDS             complement(<1..232)
                     /locus_tag="PK977_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF20294.1"
                     /translation="MGDVQVKKQPKKYDVVIVGSGAGGGMAAYVLAKAGLKICLLEAG
                     YMYDPQKNITQLKNPWDSPRRGASTKYRPFGDF"
     assembly_gap    495..643
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <644..>2340
                     /gene="pulA"
                     /locus_tag="PK977_19085"
     CDS             <644..>2340
                     /gene="pulA"
                     /locus_tag="PK977_19085"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HRF20295.1"
                     /translation="KGSFYVFRVKINGNWSDEVPDPYAKAVGVNGKRAMVVDLLQTNP
                     AGWEKDKSPVFNQKTDAIIYEPHVRDASIAANSGIGQKGKFLGLTEKGTKNADGLSTG
                     LDHLKELGITHVHLLPSYDYNSVDESVTDKPQYNWGYDPLNYNVPEGSYSINANNGAV
                     RIKEFKQLVKTFHDNGLRVIMDVVYNHTALTEKSNFNQLVPGYYYRQDEKGKFSDATA
                     CGNETASERAMMRKFILESVMYWVKEYHVDGFRFDLMGVHDIETMNLISKELHKIRPD
                     ILLYGEGWTAGKSPLPDERRALKANAAKLDRIAVFSDDIRDGIKGSVFEHTDKGFASG
                     KPGMEESIKFGIAASVQHPQVDYSKINYSKAPYSKEPFQTITYAECHDNHVLWDKLNL
                     SASPNAMQDADPVSMRDMHKLALSIVLTSQGISFLHAGTEFLRTKQGVENSYKSPDEI
                     NAIDWSLKSENKDVFDYIKGLIELRKKHPAFRMSSAEQIRKNLVFFEKQESGIVGYEL
                     NGAAAGDSWKKIQVWFNGSGEEKTVNTGREKWTSVVKDNLFEKKDNLTGTIKLKPYS"
BASE COUNT          697 a          416 c          507 g          571 t
ORIGIN      
        1 caaaatcacc aaagggcctg tatttagtgc tggctccgcg gcggggcgaa tcccatgggt
       61 tttttaattg tgtaatattt ttttgcgggt catacatata accggcttca agcaggcaaa
      121 ttttcaaacc agcttttgcc aatacatatg cagccatacc gccgccagca cccgaaccta
      181 caatgaccac atcatatttc ttcggttgtt tttttacctg gacatctccc atacggcaat
      241 aattgatatt atttcaaata aaaagacgat ttacaggttt gctcctgagc ggccgaaaaa
      301 agcagcggga atttcttcac agtttcctat tgttgcattt actaatttta aaaaaagatt
      361 ttttaatgaa gagatatatc atgctgatcg gacttattac acagttattt tcagcgaaaa
      421 cacaaaaagt ggattttagt acttacccgg tttatgatgg caatgacctg ggtcttaact
      481 acactccaaa atcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagggaa gtttttatgt
      661 atttcgtgta aaaataaatg gcaactggtc cgatgaagta cccgatcctt atgccaaagc
      721 tgttggtgtg aatgggaaaa gggcaatggt ggttgacctg ctgcaaacta atcctgcagg
      781 atgggaaaaa gataaatcac cggtttttaa ccaaaagact gatgcgatca tctatgaacc
      841 gcatgtaaga gatgccagta ttgcggctaa ttcgggaatc gggcaaaaag gtaaatttct
      901 gggcctaact gagaagggga caaaaaatgc agacggactt tctacaggac tggatcattt
      961 gaaggaactg ggcatcactc atgtgcacct gctgccttct tatgattata attcagtgga
     1021 tgaatcggtg acggacaaac cgcaatacaa ctggggctac gatcccctga actacaatgt
     1081 gcctgaagga tcctattcaa tcaatgcaaa caatggcgct gtcagaataa aagaattcaa
     1141 gcaactggtg aaaacatttc atgataatgg cctgcgggtg atcatggatg tggtatacaa
     1201 ccacacggcc ctaacagaaa aatccaattt taaccagtta gttccgggct attattaccg
     1261 gcaggatgaa aaaggaaaat tctccgatgc cacagcctgt ggcaatgaaa cagccagtga
     1321 aagggcgatg atgcggaagt ttattctcga atcggttatg tattgggtaa aagaatacca
     1381 tgtagatgga tttcgtttcg acctgatggg agtacatgat atagaaacca tgaatcttat
     1441 ttcaaaagag ctgcataaga taaggcctga catactattg tacggagagg gctggacagc
     1501 aggtaaatcg ccattgcccg atgaacgaag agctttgaaa gccaatgcgg ccaaactgga
     1561 cagaatagca gttttcagcg atgatatcag ggatggtata aaaggaagtg tgtttgagca
     1621 tacggacaag ggttttgcca gtggtaaacc gggtatggaa gaatcgatca aattcggcat
     1681 tgcagcttca gtacagcatc cgcaggtcga ttacagcaaa ataaattact caaaagcgcc
     1741 gtattctaaa gagccttttc aaacgattac ttatgcagaa tgtcatgata accatgtttt
     1801 gtgggataaa ttaaatcttt ctgcttcccc caatgccatg caggatgcag accctgtcag
     1861 tatgagagat atgcacaaac tggccttatc aatcgttctg acatctcagg ggatttcttt
     1921 tttacatgca ggtactgaat ttttgcgcac caagcaagga gtagaaaatt cttataaatc
     1981 acctgatgag atcaatgcca tcgactggtc acttaagtcg gaaaataagg atgtgtttga
     2041 ctatataaag ggactcatag agcttagaaa gaaacaccct gcgttccgga tgtcatcggc
     2101 agaacagatc cggaagaacc ttgtattctt cgaaaaacag gaaagcggaa tcgtcgggta
     2161 tgaactgaat ggtgctgcgg ccggggatag ctggaaaaag atacaggttt ggtttaacgg
     2221 gtcaggggag gagaaaacgg taaatacagg tagagaaaaa tggacttctg ttgtgaagga
     2281 taatcttttt gaaaagaaag ataacctgac tggtacaatt aaattgaaac cctacagctg
//
LOCUS       DAORLS010000408         2310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25041_length_2310_cov_1.423947, whole genome shotgun sequence.
ACCESSION   DAORLS010000408 DAORLS010000000
VERSION     DAORLS010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25041_length_2310_cov_1.423947"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>321
                     /locus_tag="PK977_19090"
     CDS             <1..>321
                     /locus_tag="PK977_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20296.1"
                     /translation="SQYLLNTYGYVTGPYDNYTPNIERTKYLGRLDWNISSKHNLSVR
                     YSQVEGGEPNSPSSSTTGAGFTYSSGLGRTNNNNLWYQNSNYFQGANFYSFAAELNSK
                     FGKFS"
     assembly_gap    322..343
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <344..>744
                     /locus_tag="PK977_19095"
     CDS             <344..>744
                     /locus_tag="PK977_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20297.1"
                     /translation="GTFTYQNDSRTTTSPKTDPAKTGGVAYDMPFVDILSTTGVANPA
                     PYVSFGYEPFSFGNLRKVKVYSIVDNLSWTTNKHNWTVGGQFELSETVNGFQRFATSY
                     YVFNTWNDFVTGQKPRDFALTYSLSKNFAPA"
     assembly_gap    745..845
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <846..>1122
                     /locus_tag="PK977_19100"
     CDS             <846..>1122
                     /locus_tag="PK977_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20298.1"
                     /translation="TYPSVPQILTHPLVAQLNFAGGETMNTGNLPKKTIMWSPRLGFN
                     WDLYGDRSLQIRGGTGIFTGRVPFVWIVSQSGDNGMIQVTQSFNGVAN"
     assembly_gap    1123..1216
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1267..>2310
                     /locus_tag="PK977_19105"
     CDS             1267..>2310
                     /locus_tag="PK977_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20299.1"
                     /translation="MDTKLPWGMVATLEAIFNKDLNTAFFRNPNLLAPAALNTTGYPD
                     RRPVYGATVPTRFINTLTNAGTVAPGAPNAFNPIILDNANRGYYFSLTAKIEKPFSKG
                     FFASVAYTKSLAGNLFDGNGDQPQSAWQSTATVAGSNYEEMSYADYVVPDRVIAVMSY
                     RKEYLKHLATTISLFYNGSINGRFSYVYGGDFNRDGVQGNDLLYIPTAAEVQQMSFAS
                     QTINGVVYDQTAQRNLFESYIQQDKYLKAHRGQYAERNGAQLPWLNRVDVKFVQDIFA
                     KTGKNRNTLQFTMDIFNFGNLINPGWGKVKSINAASILTVANNTAYSPGGTVLPQFRL
                     ATDRGQIITRTFRD"
BASE COUNT          589 a          487 c          436 g          581 t
ORIGIN      
        1 agccagtatt tgcttaacac ctatggttat gttaccggcc cttacgataa ctacactcct
       61 aatattgaaa gaacaaaata tctcggtcgc ttagactgga acatcagcag caaacacaat
      121 ttaagtgttc gctacagcca ggtggaaggc ggtgaaccaa acagccccag ttcttctaca
      181 accggtgctg gttttacata cagctctgga ttaggcagaa caaacaataa taacctttgg
      241 taccagaatt ctaactactt ccagggtgcc aatttctatt cctttgctgc cgagctgaat
      301 tcaaaatttg gtaaattctc cnnnnnnnnn nnnnnnnnnn nnnggtacat ttacctacca
      361 gaacgattca agaaccacta caagtccaaa gacagatccg gctaaaacag gaggagtggc
      421 ttatgatatg ccttttgttg atatcttaag tacaacagga gtagctaatc ctgctccata
      481 tgtttcattt ggatatgagc ctttcagctt tggcaacctg agaaaagtaa aagtatattc
      541 aatcgttgat aacctaagct ggactaccaa taaacacaac tggacagtag gtggccaatt
      601 cgaactgagt gaaactgtga acggtttcca gcgttttgct accagttact acgtatttaa
      661 tacctggaat gattttgtaa ctggccagaa gcccagagat tttgcattga cttattcttt
      721 gtcaaagaat tttgctcctg cattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnaacct atccttctgt tccgcagatc ctgactcacc cgctggtggc tcaattgaac
      901 tttgccggtg gcgaaacgat gaatacaggt aatcttccca agaagacaat catgtggtct
      961 ccccgcttag gtttcaactg ggatctttat ggcgaccgtt cacttcagat ccgtggtggt
     1021 acgggtatct tcaccggaag ggttccattt gtgtggatcg taagccagtc aggcgacaat
     1081 ggtatgatcc aggtaacaca gtctttcaac ggtgttgcta atnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnncatt ggatccaaac tttaaaaacc cacagtcatg gaaatctacc
     1261 ctggcaatgg atactaaatt accatggggt atggttgcta cattagaagc catctttaat
     1321 aaagacctga atacagcttt cttcagaaac ccgaacctgt tggctcctgc tgcattgaat
     1381 accacaggtt atcctgacag aagacctgtt tacggcgcta ctgttccgac aaggtttata
     1441 aacacactga ccaatgctgg tacagttgct ccgggtgctc cgaatgcatt taacccgatc
     1501 atcctggata atgctaaccg cggttattat ttctctctca ccgctaaaat tgaaaagcct
     1561 ttcagcaaag gattctttgc ttcagtagct tatacaaaat cactggcagg caacctgttt
     1621 gatggtaatg gtgaccagcc ccagtctgca tggcagagta cagcaactgt agctggttct
     1681 aactacgaag aaatgagcta tgctgattac gtagttcctg accgtgtgat tgctgtaatg
     1741 tcttaccgta aagaatacct gaagcacctg gcaactacca tctcattgtt ctacaatggt
     1801 tctatcaacg gccgcttctc ctatgtatat ggtggtgact tcaacaggga cggtgttcag
     1861 ggtaatgacc tgctttatat tcctactgcc gctgaagtgc agcaaatgtc atttgcttct
     1921 caaactatca acggtgttgt atatgatcag accgcacagc gtaacctgtt tgaatcttat
     1981 atccagcagg acaaatacct gaaagctcac cgtggacagt atgctgagcg taacggtgca
     2041 caattgccct ggctgaaccg tgtggatgtt aaatttgtac aggacatctt tgctaaaacc
     2101 ggcaaaaaca gaaatacttt acagttcact atggatatct tcaacttcgg taacctgatc
     2161 aatccaggct ggggtaaagt aaaatctata aatgctgcct ctatcctgac tgttgccaac
     2221 aacacagcat acagcccggg tggtaccgta ttgccccagt tcaggctggc tacagaccgt
     2281 ggacagatca tcacccgtac tttcagagat
//
LOCUS       DAORLS010000409         2307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25079_length_2307_cov_1.914742, whole genome shotgun sequence.
ACCESSION   DAORLS010000409 DAORLS010000000
VERSION     DAORLS010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25079_length_2307_cov_1.914742"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>281
                     /locus_tag="PK977_19110"
     CDS             <1..>281
                     /locus_tag="PK977_19110"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRF20300.1"
                     /translation="FGALLVLNRLKVHSLIPYLLGGVVMWYCMLQSGVHATITGVLLA
                     FAIPFGDGKEKSPSYKLQHFLHIPVAFIIVPLFALANTGILLNTPMGEV"
     assembly_gap    282..515
                     /estimated_length=234
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(658..1059)
                     /locus_tag="PK977_19115"
     CDS             complement(658..1059)
                     /locus_tag="PK977_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20301.1"
                     /translation="MHKNNAVNLGTNKRNMQQRLTFFLVVFLLMFSDGGFAQQKPYYC
                     LIETNFTPVTKLSLGTNKVLYIDYLPGKILSQPLIQQPVSANYYTRHLGLFCRKEWQL
                     EKAVSLPLRFRLGSLEYVNWLEQKPNAHKPR"
     gene            1105..>2307
                     /gene="rpoN"
                     /locus_tag="PK977_19120"
     CDS             1105..>2307
                     /gene="rpoN"
                     /locus_tag="PK977_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRF20302.1"
                     /translation="MALSQSLQQKLLQKLSPQQIQLMKLLQVPTANLETRIKEELEEN
                     PALELDEEKHDEGADELKDEFSENGEEDFEEMDGSEDEYDNIDVSEYVQDGDDEVGDY
                     KLRDDNYPEEDEGRQLPFKTETSFYEMLINQLGLLKLEEKEQTIAEQIVGSIDEDGYL
                     RRETAAIVDDLAFRQNIIATEQEVEALIKRIQQFDPPGVAARNLQECLLLQLKRQKDE
                     GKQVDIAIKAIGKYFDEFTKKHYEKIQRGLNLNDEQLREVMQQIIKLNPKPGGNVGEI
                     NKAENYVVPDFFIINNGGKLELTLNSRNAPELRISEGYRDMLKEYDRGAKKDKRQKEA
                     VLFIKQKIDAAKWFIDAIKQRQHTLLSTMNAIMEHQYNFFLTGDETELKPMILKDIAE
                     KTSLDISTV"
BASE COUNT          639 a          394 c          516 g          524 t
ORIGIN      
        1 atttggtgct ttgctggtgc tgaaccggct gaaagtacat tcgttgattc catacctgct
       61 tggcggcgtt gtcatgtggt attgtatgct gcagtcgggg gtgcatgcca ctattaccgg
      121 tgtgctgctg gcctttgcta ttccttttgg tgatggaaaa gaaaaatcac cttcttataa
      181 actgcagcac tttttgcata tacctgtggc ttttattatt gttcctctat ttgcattggc
      241 caataccggt attctgctga atacaccaat gggtgaagtg cnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctga tggtgcagta ttccaaaatc
      541 tccatactgg ttgcctcctt gctggcaggt gctgcaggat atatactgat tagcaatgca
      601 actgctaagc atgttatgga agtagaagaa gaaactgcgg aaatcagtag ttgattttta
      661 ccttggctta tgggcattcg gtttttgctc cagccagttc acatactcca gcgaacccag
      721 ccgaaaacga agcggtaatg aaactgcttt ttccagttgc cattcttttc tacaaaaaag
      781 accgagatgc cttgtgtaat aattggctga tacaggttgt tgaattaaag gttgggaaag
      841 aattttgccg ggcaggtagt ctatatataa aaccttattg gtaccaagag aaagtttggt
      901 aacaggggta aaatttgttt caattaaaca gtaatagggt ttttgctgcg caaaaccacc
      961 gtcagaaaac attaacagga acacaacaag aaagaaagtg agccgttgct gcatgtttct
     1021 tttatttgtt cctaaattta cggcattatt tttgtgcaaa ggcgaaattt ttttacgcca
     1081 cctgattatt aaacagacga accaatggcc cttagccaga gcttacagca gaaattactg
     1141 caaaaactgt cgccccagca gattcagtta atgaaactgc tgcaggtgcc tacggccaat
     1201 ctggaaacaa ggatcaagga ggaactggaa gaaaacccgg ccctggaact ggatgaggaa
     1261 aagcatgatg aaggggcgga tgaactgaaa gatgaattca gtgaaaacgg agaagaggac
     1321 tttgaggaaa tggatggcag cgaggatgaa tacgacaata tagatgttag cgaatatgtg
     1381 caggatggcg atgatgaagt gggcgactat aaactgcgtg atgataatta ccctgaagaa
     1441 gatgaaggca ggcaattgcc ttttaaaaca gagaccagtt tctacgaaat gctgatcaac
     1501 cagctgggcc tgctgaaact ggaagaaaag gagcaaacca ttgccgaaca gatcgtagga
     1561 agtattgacg aagatggcta cctgcgcagg gaaactgccg ctatcgttga tgaccttgct
     1621 tttcgccaaa atattatagc tacggaacag gaggtagagg cattgatcaa acgtatccag
     1681 cagtttgatc ctcctggtgt ggctgcccgt aacctgcagg aatgtttgct gctgcagtta
     1741 aaacggcaga aagatgaggg caaacaggtt gatatagcca tcaaagccat tggcaaatac
     1801 ttcgatgagt ttaccaaaaa gcattatgaa aagatacagc gggggctgaa cctgaatgat
     1861 gagcaactac gggaagtgat gcagcagatc attaagctga accctaaacc cggtggtaat
     1921 gtaggagaga ttaataaggc agaaaactat gtggtgccgg atttcttcat tatcaataac
     1981 ggtggtaaac tggaattgac cctgaacagc cgcaatgcgc cagaactccg tatcagtgaa
     2041 ggttaccggg atatgctgaa ggaatatgac aggggtgcca aaaaagataa gcggcagaaa
     2101 gaagctgtgc tcttcatcaa gcagaagata gatgcagcca agtggtttat agatgcgatc
     2161 aaacagcggc agcatacact gctgagtact atgaatgcca tcatggaaca ccaatacaat
     2221 ttctttttaa ccggtgatga gaccgaacta aagccgatga tcttaaaaga tattgcggaa
     2281 aaaaccagcc tggatatatc cacggtt
//
LOCUS       DAORLS010000410         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25392_length_2285_cov_2.336323, whole genome shotgun sequence.
ACCESSION   DAORLS010000410 DAORLS010000000
VERSION     DAORLS010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25392_length_2285_cov_2.336323"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PK977_19125"
     CDS             <1..739
                     /locus_tag="PK977_19125"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF20303.1"
                     /translation="ILLAVIYNQRKKDQHRKAVEQLREQQQNQLIEAAVRSEELERHR
                     IAETLHDEVGAILSSAKLHLLGIKSEVLDERDQKLHEKGRELLNEVIQKVRGISHNLH
                     SNILKEFGLNEAIRHFVKKVTQGTMLQATTALDDNYQTENAENDISIYRMVQELMNNI
                     LKYANATEFLISSTLEGNELNLVLFHNGDGLTQEQFEELRFRKEGLGLKNIQNRIILL
                     KGTIHFTQGGEGYRINIHVPVKTNNAA"
     gene            736..1386
                     /locus_tag="PK977_19130"
     CDS             736..1386
                     /locus_tag="PK977_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF20304.1"
                     /translation="MSKIKIAIADDYKIFREGLKVGLSADDNLEVMLEADNGEELLKG
                     LENNSPDVIIMDLKMPIMDGMEATKEVRKKFPSIKVLVVSMYEDDKFIIHLMENGANG
                     YLLKNAEPDEIRRSIYSVHENGYYFNDLVNKALLKKLVLKNNLKPSFNQNIDLTEREQ
                     EVLKMICEEKTAAEIAKEIFLSPRSVEGIRQRLIEKIGVRNTAGLVMFAVKNGIVD"
     gene            complement(1494..>2285)
                     /locus_tag="PK977_19135"
     CDS             complement(1494..>2285)
                     /locus_tag="PK977_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF20305.1"
                     /translation="SMDVMNKEMLHVQVIRQGITQIYLLAAILSATGEKNPNLAWNLN
                     MQGLLNVLDIAREEKLAKVYWPSSIAVFGPTSPRQNCPQQTIIEPTTVYGISKYAGEF
                     WCNYFFQRFGVDVRSLRYPGLISYKSAPGGGTTDYAVEIFHEALEEKKYECFLSEDTY
                     LPMMYMPDAIRATIELMEAPASKIAVRTSYNVSGMSFSPKEIAAEIKKHIPDFTISYK
                     PDYRQAIANSWPQSIDDTVARNDWGWKEEYNLAAMTGDMLENLGR"
BASE COUNT          699 a          567 c          497 g          522 t
ORIGIN      
        1 catcctgctg gctgttattt acaaccagcg caaaaaagat cagcacagga aggccgtaga
       61 acaactccgt gaacagcagc aaaaccaact tatcgaagcg gcggtacgca gcgaagaact
      121 ggaacgccac cgcattgctg aaacactgca cgacgaagtc ggcgctattc tctcttctgc
      181 caaactgcac ctgctcggta ttaaatcaga agtgctggac gaaagagacc agaagctgca
      241 tgaaaaagga cgggaactgc tgaacgaagt cattcaaaaa gtaaggggca tttcccacaa
      301 cctgcactcc aacatcctga aagagtttgg actcaacgaa gccatccgcc actttgtaaa
      361 gaaagtaacg cagggcacca tgcttcaggc tactaccgcc cttgatgata attaccagac
      421 agaaaatgcc gaaaacgata tcagcatata ccgcatggta caggaactga tgaacaatat
      481 cctgaaatat gccaacgcca ccgagttcct catcagctct acactggaag gtaatgagct
      541 gaaccttgta ttattccaca atggcgacgg gcttacacaa gaacagtttg aagaactccg
      601 tttccgtaaa gagggactgg gcttgaaaaa tatccagaac aggattatat tgctaaaagg
      661 aaccatccat ttcacacagg gcggcgaagg ataccgtatc aacatacatg ttcccgtaaa
      721 aaccaacaac gcagcatgag caaaataaaa atagccatag cagacgatta taaaattttc
      781 cgtgaaggat taaaagtggg cttgtcggct gacgacaacc tggaagtaat gctggaagcc
      841 gacaacggcg aagaattgct aaaaggcctc gagaataatt ctcctgatgt catcatcatg
      901 gatctcaaaa tgcccatcat ggatggcatg gaagccacca aggaagtgcg aaaaaaattt
      961 ccatccatca aagtgctggt ggtaagcatg tatgaggatg acaagttcat catccacctg
     1021 atggaaaacg gtgctaatgg ttacttattg aaaaatgcag aaccggatga aatacgccgt
     1081 tccatttatt ccgtgcatga aaacggttat tacttcaacg acctggtgaa taaggccctg
     1141 ctgaaaaagc tggttttgaa aaacaacctc aaaccctcct tcaaccagaa tatagacctt
     1201 acggaaagag aacaggaagt actcaaaatg atctgcgagg aaaaaacagc agcagagatt
     1261 gccaaagaaa ttttcttaag cccccgctct gtagaaggta tccgtcagag gctaattgaa
     1321 aaaatcggtg tacgcaacac cgccggcctc gtcatgtttg cagtaaaaaa cggaattgtt
     1381 gactaacatc ggctgtcatg ctgagctcag tttaccctga ggaaccgaag ggtcgaagca
     1441 tgaaatcact attgactatt gactcctgac tatcgactcc ctactcctgc ctcctacctc
     1501 cccaaattct ccagcatatc ccccgtcatt gcagccaggt tatactcttc cttccagccc
     1561 cagtcattcc tcgctaccgt atcatcaata ctctgcggcc agctgttggc aatggcctgc
     1621 cggtaatcag gtttgtagct gatggtaaaa tcaggaatat gctttttgat ctccgccgca
     1681 atttctttgg gagaaaaact catacctgaa acattgtaag aagtacggac tgcaattttg
     1741 gaagccggcg cttccatcag ttcgatggta gcccttatcg catccggcat atacatcatc
     1801 ggcaggtagg tatcctcaga caggaaacat tcatatttct tttcttccag tgcctcatga
     1861 aatatctcca ccgcataatc cgtagtacca ccaccgggag cagatttata agagatcaaa
     1921 ccgggataac ggagactccg tacatccact ccaaaacgct gaaagaaata attgcaccaa
     1981 aactcaccgg catatttgct gatgccatac accgtagtcg gttcaatgat ggtttgctgc
     2041 gggcagtttt gcctagggga agtagggcca aacaccgcaa tagaagaagg ccagtacact
     2101 ttggccagct tttcttctct ggcaatatcc agcacattca gcaggccctg catattcagg
     2161 ttccaggcaa ggttggggtt tttctcgccg gtggcggaaa gtatcgccgc cagcaggtag
     2221 atctgtgtaa tgccctgccg tattacctgc acatgcagca tctctttgtt catcacatcc
     2281 atgct
//
LOCUS       DAORLS010000411         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25395_length_2285_cov_1.747982, whole genome shotgun sequence.
ACCESSION   DAORLS010000411 DAORLS010000000
VERSION     DAORLS010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25395_length_2285_cov_1.747982"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>258
                     /locus_tag="PK977_19140"
     CDS             <1..>258
                     /locus_tag="PK977_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20306.1"
                     /translation="GNAAYNPLLIENGSGHNWSARVIDGLTASPGYNTDRAVLLQWDI
                     TPSVNPPAGGADITFQFDQSTQVGPLFNTGTSVQAWRNPLDA"
     assembly_gap    259..321
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <322..951
                     /locus_tag="PK977_19145"
     CDS             <322..951
                     /locus_tag="PK977_19145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20307.1"
                     /translation="LQRTGMSGFSQFAVGGQSNLSLPVTLLSFSGYKDGSRNQLSWTT
                     GTEINNRGFDVQRSADGINYTSIGFVNSLATGGNSNDQLSYRFTDNTPAGVKQYYRLR
                     QEDLDGRSKLSNIVLINGARPTAIAIRSLFPNPASVTASMIVDAPSAAKLTVLVSDMA
                     GRNMVQQIVNVSEGSNMVALNIASLQSGTYLVRVMSETGEVVTGKLVKQ"
     gene            1408..>2285
                     /locus_tag="PK977_19150"
     CDS             1408..>2285
                     /locus_tag="PK977_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20308.1"
                     /translation="MKKFLPVILFFVASNFSFSVKTTAQSSCPQIINSTFRIITNQGN
                     QTRKRISFDYINPSNGNKRISVLVTVNGTSVVNVCVNASNYRGVQRTYTTPEFTVTNM
                     ANMDVTITPYTASNCGGSACAPTIRSVGGAPLPVIFSAFTLARNNATVSVKWETATEI
                     SNSGFNVEKFNSNNEEWEPVAFVRSKAEGGNSSNRLSYEYNDINNSKAVSQYRIKQVD
                     INGEFKYSEVRTIRGMEQDARTMVFPNPSSNGTVNVVFAEASVRDIILVDMAGRTIKQ
                     WNAYSNNSLQLNNLVPG"
BASE COUNT          659 a          465 c          507 g          591 t
ORIGIN      
        1 ggcaatgcgg cttacaatcc attgcttata gaaaacggca gcggtcataa ttggagtgca
       61 agggtgattg acggattaac agccagccca ggttataata ccgatagagc tgttttactg
      121 caatgggata ttacaccttc tgtaaaccct cctgcaggtg gcgctgatat tactttccag
      181 tttgatcaaa gtacacaggt agggccgctg tttaacaccg gtaccagtgt gcaggcatgg
      241 cgcaacccgc tggatgcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nttacagcgc accggcatga gtggtttttc acagttcgcc
      361 gttggtggac aaagtaacct ttcattaccg gtaacattac tcagcttctc cggttacaaa
      421 gacggcagcc gcaaccagct cagctggact accggcacag aaatcaataa ccgtggtttt
      481 gatgtgcagc gttcagcaga tgggatcaat tatacttcca tcggttttgt aaacagcctt
      541 gccaccggtg gcaacagcaa tgaccagctc agctatcgct ttacggataa tacaccggct
      601 ggagtgaaac aatactaccg cttgcgccag gaagatttag atggccgcag caagctcagc
      661 aatattgtgc tgataaatgg tgcgagaccg acagccatcg ccattagaag cctattccct
      721 aacccggcat ctgtgacggc cagtatgatc gttgacgcac catctgcagc caaactcacg
      781 gtgctggtat cagatatggc cggccgtaat atggtgcagc aaatagtgaa tgtatcagaa
      841 ggaagcaata tggtagcgtt gaatattgct tcactgcaaa gtggtactta tttagtgagg
      901 gtgatgagtg agacaggtga agtggtaacc ggcaaactgg tgaagcagta gagattggag
      961 tcgtgagtca tttttgccat gtttcgacaa gctcaacatg acaacatcac tcagccaggc
     1021 aaaagttgtt agaacgggtt atcagaaaaa ccaaataatg gaatccgcct ccgtaagggg
     1081 gcggattttt tatttaaaat cggaaaagat gaaaacagga tatcgtaaaa gttgaaattg
     1141 attgaaggga ttttcccatt gcgtttcttt atccagaaga tgaatcaatt ctttctgtat
     1201 ttgataagaa tagaaaaatg taggtgtaac ttcttatcag gagtttagta ttttcttcat
     1261 ttacgtagaa ttactaataa aaattctcgt aaaagatgtt gtttcaaata gtaactcatg
     1321 ctatttttgc ggagcattta cgggaaaaca ccctataagt accgtaaaaa ccctaagtaa
     1381 tccgtaacta aagaaaacaa ccgtaccatg aagaaatttt tacccgtcat tctttttttt
     1441 gtagcaagca atttttcatt ctcagttaag acaactgctc aaagttcttg cccccagatc
     1501 atcaattcta ccttccgtat tatcaccaat caaggcaacc aaacccgcaa gcggattagt
     1561 tttgattata tcaatccttc aaatggcaat aaacgcatca gtgtattggt gactgtgaat
     1621 ggtaccagtg tagttaatgt ttgtgtgaat gcttccaatt atcggggtgt acagcgtacc
     1681 tatactacac ctgagtttac agtaacgaat atggccaata tggatgttac aattacccct
     1741 tacactgcaa gcaactgcgg cggtagcgcc tgtgcaccta ccattcgttc cgtcggtggt
     1801 gcaccgctgc ctgtaatatt ttctgcattt acccttgcca gaaataatgc aactgtatct
     1861 gtaaaatggg aaactgcaac agagatcagc aatagcggtt ttaatgtcga aaagtttaac
     1921 agcaacaatg aagagtggga gccggtagct tttgtacgtt caaaggcaga gggtggtaac
     1981 agcagcaacc gcctgagcta tgagtataat gatatcaata acagcaaagc tgtatcccag
     2041 tatcgcatta aacaggttga tatcaatggg gaattcaaat acagcgaagt aaggactatc
     2101 cgcggtatgg agcaggatgc caggacaatg gttttcccta accccagcag caatggtaca
     2161 gtaaatgtgg tgtttgctga agcttctgtt cgtgacatta tactggtgga tatggccggc
     2221 cgtacaataa agcagtggaa tgcttacagc aacaacagcc ttcagttgaa taaccttgta
     2281 cctgg
//
LOCUS       DAORLS010000412         2282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25428_length_2282_cov_1.750337, whole genome shotgun sequence.
ACCESSION   DAORLS010000412 DAORLS010000000
VERSION     DAORLS010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25428_length_2282_cov_1.750337"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..>1904)
                     /gene="lepA"
                     /locus_tag="PK977_19155"
     CDS             complement(164..>1904)
                     /gene="lepA"
                     /locus_tag="PK977_19155"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRF20309.1"
                     /translation="TLADRLLQQTNTISDRDMMDQVLDDMDLEREKGITIKSHAIQIN
                     YKHTDGQEYILNLIDTPGHVDFSYEVSRALAACEGALLLVDATQGIQAQTISNLYLAI
                     DNDLEIIPVINKIDMDGAMIEEVEDQIIDLIGCKKEDILHASGRTGLGVDKILDAIVT
                     RIPSPKGNPDAPLQALIFDSVFNSFRGIIVYYRILNGSIKKGDKVKFVSTGQEYEADE
                     VGILKLKMTEKQEVKTGDVGYIITGIKNAKEVKVGDTITLANNPNPEQIRGFEEAKPM
                     VFAGIFPVNTDEFEELRDCMDKLQLNDASLTYELETSQALGFGFRCGFLGMLHMEIIQ
                     ERLEREFNQTVITTVPNVSFIAYTTKGEKVIVNNPTEFPDPVKTDRIEEPFIKAQIIT
                     KPDYIGNIMTLCLGKRGILMNQSYLTQTRVELIFEMPLTEIVFDFYDKLKSQTRGYAS
                     FDYHPIGYRDSDIVKMDILLNGDKVDALSALIHRSRAQDFGRKLCEKLKELLPRQQFQ
                     IAIQAAIGAKVVARENISAMRKDVTAKCYGGDISRKRKLLEKQKEGKKRMRQIGNVEV
                     PQEAFLAVLKLDD"
     assembly_gap    1905..1932
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          604 a          524 c          422 g          704 t
ORIGIN      
        1 tgacagagcc tgcaaatctt taagcttcga aagcctaact actttcagtc caaagactcg
       61 taaaggataa ttaagtatat ctgaccattt cataaaccga agcgaaacta aaataaaaag
      121 gcatcatatc agatgccttt acaaaataga atgtttaata atcttaatca tccagcttca
      181 aaacagccag gaacgcttcc tgcggcactt ccacatttcc gatttgtctc atacgtttct
      241 tgccctcttt ctgtttttcc agcagtttac gtttacggct gatatcacca ccataacatt
      301 tggcggttac atcttttcgc atcgctgaaa tattttctct tgctaccacc tttgccccta
      361 ttgctgcctg aatggcgatc tggaattgtt gtctcggtaa caactctttc agcttctcac
      421 acagctttct gccaaagtcc tgtgcacggc tacggtgtat cagcgctgat aaagcatcga
      481 ccttgtcacc attcagcagt atatccattt ttacaatatc gctgtcacgg tacccgatcg
      541 ggtgatagtc aaacgaagca tagcctcttg tttgtgattt tagcttatcg taaaaatcga
      601 aaacgatttc tgttaagggc atttcaaaga tcagctctac ccttgtctgg gtaaggtagc
      661 tttgattcat taagattcct ctttttccca ggcaaagggt catgatattg ccaatatagt
      721 caggctttgt aatgatctgt gccttgatga atggttcttc tatcctgtct gtttttaccg
      781 gatcagggaa ttctgtcgga ttatttacaa ttaccttctc tcctttcgtg gtgtaagcaa
      841 taaaacttac gttaggaacg gttgtgatta ccgtctggtt aaattctctt tccagccttt
      901 cctgtatgat ctccatgtgt agcattccta aaaagccgca acgaaagcca aaaccaagag
      961 cctgagatgt ttccagttcg tatgtcagtg aagcatcatt caattgcagt ttatccatgc
     1021 aatccctgag ttcttcaaac tcatctgtat ttacgggaaa gataccggca aataccatcg
     1081 gtttcgcttc ctcaaagccc cttatctgct caggattagg attattggca agtgtgattg
     1141 tatcacccac tttaacctct tttgcatttt taatgccggt aatgatatag cctacatcgc
     1201 ccgtctttac ctcctgcttc tcggtcattt tcagcttgag gattcccacc tcatcagcct
     1261 cgtattcctg cccggttgaa acaaacttta ctttatctcc ttttttaatc gaaccattaa
     1321 ggatacgata gtaaactata atacccctga atgaattaaa aacggaatcg aatatcaggg
     1381 cctgtaacgg ggcatccggg tttcccttgg gtgaaggaat acgggtaaca atagcatcaa
     1441 gaatcttatc tacacccaaa ccagtacggc cactggcatg cagtatatct tcctttttac
     1501 aaccgataag gtcaatgatc tggtcttcca cttcctcgat catcgcaccg tccatatcaa
     1561 ttttattgat taccggaata atctccaggt cgttgtcaat agccaggtaa agattactta
     1621 tcgtttgtgc ctgtataccc tgggtagcat caaccaacag caaagctcct tcacaagctg
     1681 caagggctct gcttacctcg taactaaagt ccacatggcc gggagtatca atcaggttca
     1741 ggatatactc ctgcccgtct gtatgtttat aattgatctg gatagcatga cttttaatgg
     1801 taatgccctt ctcccgttcc aggtccatat catccagcac ctggtccatc atatccctgt
     1861 cacttatagt attggtctgt tgcaaaagac ggtcagccag ggtgnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnggtcaata tgtgcaataa tgcagaaatt ccggatattc ttcatgtacg
     1981 ttcttgcctc aattttagga tgggcgaagg tacggaaaag aggcggtttt taagggattg
     2041 tttagcagcc tattaccttc cgtttaaaga ggaaaggagg gtcataaaat caataattgt
     2101 gatattttca cccttaaaat tctctaatgg gcaaagatta cattaccgac cgggtttatg
     2161 agaaaacaga ttttacaata accgacctcc caaaagcgga ttttgagggt tgccgcttcg
     2221 cccactgcaa cttttctggt tccaatttat caggctataa tttcacagaa tgtgaatttg
     2281 aa
//
LOCUS       DAORLS010000413         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25485_length_2278_cov_1.962663, whole genome shotgun sequence.
ACCESSION   DAORLS010000413 DAORLS010000000
VERSION     DAORLS010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25485_length_2278_cov_1.962663"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PK977_19160"
     CDS             complement(<1..1324)
                     /locus_tag="PK977_19160"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF20310.1"
                     /translation="MNKFLLSLILGSFLCICLAQQRVNTDNVALKKNYDLANKLFQQA
                     ELLSLKAGDNEKLYEAADQHYRESLATFDKLLKEEEKNLSDSLLFWIKTRKAYIEYYF
                     DSLETAKTDYLAAILLKKKLRSLSDSALFTPLLYTGGIYYTHDQFDSALYFYKQAELF
                     LEKHKKPLRETQRLYNRLGVMQYETGNYRQAKNYFEKAIAVQGADPAADKSLLHNYRI
                     NIASLHVKLEEYEQARTIYEGLLPSADFANEIYHNLGIICIKTGDFKKGIDYFRKVTY
                     PDNKKLIDLFYNFSVAWAGLREADSAELYLHKALAENLKWNGHRKNTSFGLIQKYQGD
                     ELKKLQKFREAAGQYQQAIIQFCIDFNESDISKNPVDFTSAFSYINLFAALSAKAEVL
                     EQQFRINKDINLLVTAVNTYETAFNLADYVGKNYTSDESRMFLGKIKHT"
     gene            complement(1364..2173)
                     /locus_tag="PK977_19165"
     CDS             complement(1364..2173)
                     /locus_tag="PK977_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20311.1"
                     /translation="MNCPKPILGVICILFSMVWIGCDKSSGTADDNIPYNLSYGDSII
                     YLKPSAGDYIINPLVHREGVYTGFPDGIEIDDQTGAINVSKSETGLRYRITHTSPQGR
                     MTTTMVVLSGITFTDHFYRLATGDSVAHPVYNASTDRILPLNGSIFDEDNLANGGGCA
                     VRTYDGKINLAESIRKGVFGANPQNDDKKEIEIKYRINDGSGKTLNKLKVLLYWYNTM
                     ADVPQYVWEILNDRANQGVFLRNGNIATNNTSLQIEKEARPRPPCVIIIAN"
BASE COUNT          626 a          437 c          468 g          747 t
ORIGIN      
        1 cggtatgctt gatctttcca aggaacattc ttgattcatc cgaagtgtaa tttttcccca
       61 cataatcagc caggttaaat gcggtttcat aagtattgac agcagttacc agcaggttta
      121 tatccttgtt gatcctaaat tgctgttcca atacttcggc ttttgcagat aaggcagcaa
      181 aaagattgat ataggaaaat gcactggtaa aatcaaccgg gtttttggat atatcactct
      241 cattaaagtc aatacagaac tgtattatcg cctgctggta ttggccggca gcttccctga
      301 acttttgcag tttttttagt tcatcaccct ggtatttttg aatcaggcca aaactggtat
      361 tttttctatg gccattccat ttcaggtttt cggcaagggc tttatgaaga tataactctg
      421 ccgagtcggc ttcacgaagg ccggcccagg ccacactaaa attatagaag agatcaatta
      481 gttttttatt atcagggtag gttacttttc ggaaatagtc aattcctttt ttgaaatctc
      541 ccgtttttat gcaaataata ccaaggttat gatatatttc attggcaaaa tctgcagagg
      601 gtagcaaacc ttcgtagata gttctggcct gttcgtattc ttcgagttta acgtggaggg
      661 aagcgatatt gattcgataa ttatgtaata gacttttatc ggctgccggg tcagctccct
      721 gaacagcaat ggctttttca aagtagtttt ttgcctggcg ataattaccg gtctcatatt
      781 gcattactcc cagacggttg taaagccttt gcgtttcccg gagcggtttc ttgtgttttt
      841 cgagaaagag ttcggcttgt ttataaaaat aaagtgcaga atcaaactgg tcatgggtat
      901 agtaaattcc gccagtgtat aacagtggag tgaaaagggc tgagtccgaa agagacctaa
      961 gtttcttttt taataagatg gcagcgaggt aatcggtctt ggcagtttcc aggctgtcaa
     1021 aataatattc tatatacgct tttctggtct taatccagaa taaaagggag tcagatagat
     1081 ttttttcttc ctcttttagt aatttatcaa aagttgcaag ggattcccgg taatgctggt
     1141 ctgcagcttc atataatttt tcattatcac cggctttcag tgacagcagt tcggcttgct
     1201 ggaaaagttt gttagctaaa tcatagtttt ttttcagagc tacattgtca gtattcactc
     1261 tctgttgtgc taaacaaatg catagaaaac tgcccaatat gagggaaagc aaaaatttat
     1321 tcatgataag ctgtgatagc ctatcaaatt taattgtgaa agcttaatta gcaataataa
     1381 tcacacaggg aggccgtggt cttgcctctt tttcaatttg gaggcttgta ttgtttgtgg
     1441 ctatgttccc atttctcaga aaaactccct gattagccct gtcattcaaa atttcccaca
     1501 catactgtgg tacgtcagcc attgtattgt accaatacag caaaacctta agcttattca
     1561 gcgttttgcc actgccatca tttattcggt atttgatttc aatttccttt ttatcatcat
     1621 tttgcgggtt ggcaccaaat actccttttc ggatcgattc agcaaggttt atttttccat
     1681 catatgttct gactgcacat ccgccgccat tagcaagatt gtcttcgtcg aagatggagc
     1741 cgtttaatgg taaaattctg tcagtacttg cattgtaaac agggtgtgct acggaatcgc
     1801 cggtagccag gcggtaaaaa tggtctgtaa acgtaatacc tgagagtacc accatggttg
     1861 tggtcatcct gccttgagga gaagtgtggg taatacgata tcgcagaccg gtttcgcttt
     1921 tactcacatt aattgcgcct gtttggtcgt caatttcaat tccatcaggg aaaccggtat
     1981 atacaccttc ccggtgaacc aaagggttga tgatataatc gccggctgaa ggcttcaggt
     2041 aaatgataga atcaccatag cttaggttat aggggatgtt atcatctgcg gtaccagatg
     2101 acttgtcaca gccaatccag accattgaaa ataaaatgca gataacacct aaaattggtt
     2161 ttggacaatt catgcaggcc tttttggtga gtaaacactg attattttaa tgtaaccatc
     2221 agtttaaaag tatttgaagt tacaacattt atcatgccgg aattcctgat tgaaatca
//
LOCUS       DAORLS010000414         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25608_length_2270_cov_1.897065, whole genome shotgun sequence.
ACCESSION   DAORLS010000414 DAORLS010000000
VERSION     DAORLS010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25608_length_2270_cov_1.897065"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1166)
                     /locus_tag="PK977_19170"
     CDS             complement(144..1166)
                     /locus_tag="PK977_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20312.1"
                     /translation="MKKFLFVSSFILSTFILKAQAVDSIRFFTDEPVIEMTLSTDIRG
                     LQNQKGDDIFQDGKVSMKFPDSTVITENVKVGARGKFRRGYCRIPPIMLNFRTPDGNS
                     RFQSLGKLKLVIGCGVKSTDEELLLKEFLVYKIYNLLEDKSFRVRLLRTTYEDTKGRN
                     KPFTQYSFLIEDDGDMARRNNCKKKEHGQYLTEATDRNTMTMVALFEYMISNTDWSVP
                     NNHNVKLIFDRDNEAAPPHAVPYDFDYCGLVDASYAIPHEIIGTEKVTERVYRGFPRT
                     LEEIQITLDEFRKKKSAIYALINNFNLLSTRTRKTMTDFLDEFFELIENKNRVKSVFV
                     DNARTS"
     gene            complement(1245..>2270)
                     /locus_tag="PK977_19175"
     CDS             complement(1245..>2270)
                     /locus_tag="PK977_19175"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF20313.1"
                     /translation="SSFNELKLVDEYYKKGWSLLYPANTYLQSTADEKKYEAKPFGTS
                     FPYNLSGFAGKDYGKITTTPWGNTLTTEFAKNAVINEGLGADNITDFLAVSYSSPDYI
                     GHSFGPNSIESEDGFLRFDRELGEFFDFLDKKIGKGQYTVFLSADHGVANIPEYMQEN
                     KLPGGRVFMADVIKQLNKTLAEKFKISNIVVSDDNYQLHLNHPALDSAKTDKEELIEW
                     IVDYISAEPGIDRAFAVDDLNEIPLPATIRKALNNGYYPPRNGDIQIILKPNYIEAWS
                     NTGTTHGLWNPYDSHIPLLWYGWGIRQGKSNKEVYMTDIAPTIAALLHIQMPNGSVGQ
                     VISEVLK"
BASE COUNT          606 a          458 c          449 g          757 t
ORIGIN      
        1 ataaagagaa tacagctgaa gtaaagtgtc attactcgga cgttcaggaa ggtttttgct
       61 ttcagtcact gcttgttcaa attgttcttt cagatccata cagttgttta aaggtgaaaa
      121 atgtggttag gctgtatcta tgatcagctt gttctggcat tgtctacaaa aacggactta
      181 accctgtttt tattttcgat aagctcaaag aattcatcga ggaaatctgt cattgttttc
      241 cttgtccttg tgctcaacag attgaaatta ttaatcaatg cataaatagc tgatttcttc
      301 ttgcggaatt catccagggt aatttgtatt tcttccaatg ttcttggaaa acccctgtaa
      361 acccgttcag ttactttttc agttccaata atttcatgcg ggatcgcata actggcatct
      421 accagcccgc agtaatcgaa atcataaggt actgcatgcg gcggggctgc ctcattatcc
      481 cggtcaaaga tgagttttac attgtgatta ttaggtacag accagtctgt attgcttatc
      541 atatactcaa acaatgccac cattgtcata gtgttgcggt cggttgcttc ggtaaggtat
      601 tggccatgct ctttcttttt gcagttatta cgacgggcca tatcgccgtc atcttcgata
      661 aggaaagaat attgggtaaa gggtttgttg cgtccttttg tgtcttcata agtagtcctt
      721 agtaaacgca ccctgaaact tttatcttcc agcaggttgt agattttata taccaaaaac
      781 tctttcagta ataattcttc atctgtagat tttacaccac agccaattac aagctttaac
      841 tttcccaggg actgaaaacg ggaatttcca tctggtgttc tgaagttgag cattatggga
      901 gggattctgc aatagcccct tctgaatttt ccccgggcgc cgacttttac gttttccgtg
      961 ataactgtgc tgtcggggaa tttcatggat actttcccgt cctggaatat atcgtcacct
     1021 ttttgatttt gcaggcctct tatatcagta cttagcgtca tttcgataac cggctcatcg
     1081 gtaaagaaac gaatgctgtc aacagcctgc gcttttaaaa taaaagtgct taaaataaaa
     1141 gaggatacaa aaagaaactt tttcatgatg acgagttatt ggtggttgat tagttttaat
     1201 tatggaattt acaaataaac cggcaataac agtttaatta aggtttactt caatacttca
     1261 ctgatgacct gtcccacaga accattgggc atttgaatat gtaacaatgc tgctatagtt
     1321 ggggcaatat cggtcatgta cacttcctta ttggattttc cctgccggat accccagcca
     1381 taccagagta aaggaatgtg tgagtcatag ggattccaaa gcccatgggt agtaccggta
     1441 ttgctccatg cttctatgta attaggctta agtatgatct ggatatcgcc atttctcgga
     1501 gggtaatatc cgttgttcag cgcttttctt attgtggccg gcagtggtat ctcattcaga
     1561 tcatccaccg caaatgctct gtcaataccc ggttctgcgc tgatgtagtc cacgatccat
     1621 tcgatcagtt cctctttgtc tgttttggca gaatccaaag ccggatgatt aaggtgtaac
     1681 tggtagttat catcactcac aactatattg cttattttaa atttttctgc cagggtttta
     1741 tttagctgct taattacatc tgccataaaa actctgcctc cgggtaattt gttttcctgc
     1801 atgtattcag gaatattagc cacgccatga tcggcagaaa ggaaaacagt gtattgacct
     1861 ttccctattt ttttatcaag aaagtcaaag aattctccca gttctctgtc gaagcgcaaa
     1921 aaaccatctt cactttctat tgagtttggt ccaaatgaat ggccgatata atcaggcgat
     1981 gaatagctta cagccagaaa atcagttatg ttatcagcgc caagtccttc gttgataacg
     2041 gcatttttgg caaactccgt tgttagtgta ttgccccatg gagtggttgt aatttttcca
     2101 taatctttac cggcgaaacc gcttaagttg tatgggaatg aagtgccaaa aggtttggct
     2161 tcatactttt tttcatcagc tgtgctttgc aaataagtgt tagccgggta taaaagcgac
     2221 cagccttttt tataatattc gtcaaccaat tttaattcat taaaactgct
//
LOCUS       DAORLS010000415         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25610_length_2270_cov_1.507449, whole genome shotgun sequence.
ACCESSION   DAORLS010000415 DAORLS010000000
VERSION     DAORLS010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25610_length_2270_cov_1.507449"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>256
                     /locus_tag="PK977_19180"
     CDS             <1..>256
                     /locus_tag="PK977_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20314.1"
                     /translation="VLDNVTVVATNPGLRLGIDRKVFNVDKNLVSAGGTAVDVMRNVP
                     SVNVDIDGNVTMRNNAPQIFVDGRPTNMELDQIPADAIESV"
     assembly_gap    257..273
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <274..>526
                     /locus_tag="PK977_19185"
     CDS             <274..>526
                     /locus_tag="PK977_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20315.1"
                     /translation="NPSAKFDASGGTAGILNVILKKNKRVGYNGSVRANIDSRARIGL
                     GGNFNLRQDKINYFLNVNYNQRKSISNGTTDRTTFIGNPN"
     assembly_gap    527..655
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <656..2212
                     /locus_tag="PK977_19190"
     CDS             <656..2212
                     /locus_tag="PK977_19190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF20316.1"
                     /translation="SQGRFEPYTNSDILIDSLYSTVTQSFSQRLSNSNSKFNNLGTMI
                     SYKHLFPKQGEELTADITYNKSRNSSTNLISTDYYKVPGYTLQSAFKQQQDGKGENEN
                     IVIQSDYVKPLNDKSKIELGVRAQIRKVDSRNDFFFLDNNGVAIYNPQLSVNYNSTDN
                     VYAAYTTYTNQIKDFGYQLGLRLESSDYNGQLPDKNQEFNIKFPVSLFPSVFLSQKLK
                     NNQEMQLNYTRKINRPNFFQLFPFTDYSDSLNISRGNPNLSPEFTNSLELTYQKTFKN
                     RDNFIASLYYKNTTDLISRFQAQEQDPVNGKDILVNTYINANSSYITGLELTSTNKIT
                     KWWEMTTNMNLFNSRIKIGIEGQPEQDPLTSWFGKLNNNFKLAKTLTLQVSGEYQSKT
                     ILPPSSGRGGGRGGGMFGGGGMWGMTTTSQGYIKSNYFVDAGLRFEFLKNKQASISLN
                     VNDIFRTRRQNIISESPLFYQEVFRRRDPQILRLNFNWRFGKFDANLFKRKNNRDNMG
                     DGTEGIGMRP"
BASE COUNT          661 a          441 c          445 g          577 t
ORIGIN      
        1 gtgctggata acgtaacggt tgttgctaca aatcccgggc ttagattagg aatagacagg
       61 aaagtgttca atgtggacaa gaacctggtt tcagccggtg gcaccgccgt agatgtaatg
      121 cgtaatgtac cttctgttaa tgtagatatt gacggaaatg taaccatgcg aaataatgca
      181 ccgcagattt ttgtagatgg ccgtcctacc aacatggagc tcgaccagat tcctgccgat
      241 gcgattgaaa gcgttgnnnn nnnnnnnnnn nnncaacccc tctgccaaat ttgatgcttc
      301 cggaggtaca gctggtatcc tgaatgtgat tttaaaaaag aataaaagag ttggttacaa
      361 cggaagtgtt agggctaata ttgattcaag ggcaaggata ggtttgggtg gaaactttaa
      421 cctgagacag gataagatca attacttttt aaacgtaaat tataaccagc gcaaatctat
      481 cagcaacggt accacagacc gtactacttt tattggaaac cccaatnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcaca
      661 gggccgtttt gaaccttata ccaacagcga tatattaatc gattcacttt acagcacagt
      721 aacccaatct ttcagtcagc gtctttccaa ttccaacagc aagttcaata acctgggtac
      781 catgattagt tataagcacc tttttcccaa acaaggtgag gaacttacgg ctgatataac
      841 ttataataaa agccggaatt ccagtactaa cctgatctcc actgattatt acaaagtgcc
      901 gggctatacc ttacaaagtg catttaagca gcagcaggat ggtaaaggag aaaatgagaa
      961 tattgttatt cagtctgatt atgtaaaacc tttaaatgac aaatctaaaa ttgaattggg
     1021 tgtcagggcg cagatccgta aagtagatag ccgtaatgac tttttcttcc ttgataacaa
     1081 tggggtggcc atttataatc cccagctttc tgtgaattat aacagtacag ataatgtgta
     1141 tgcggcttat actacttata ccaaccagat caaggatttt ggttaccagt tgggattaag
     1201 attagaaagt tctgattaca atggtcaact ccccgataag aaccaggagt tcaatatcaa
     1261 atttcctgtc agcctttttc cgagtgtatt tttaagccag aagctgaaga ataaccagga
     1321 aatgcagctt aactataccc gcaagatcaa caggcctaac tttttccagt tattcccatt
     1381 cactgattat tcagattcac tcaatatcag caggggtaat cccaacctga gccctgagtt
     1441 taccaattca ctggagctta cttatcaaaa gacctttaag aacagggata atttcattgc
     1501 ctctttatat tataaaaaca cgactgacct gatcagccgc ttccaggcac aggagcaaga
     1561 cccggtaaat ggtaaagaca tcctggtaaa tacctatatc aatgccaatt caagttatat
     1621 aactggtctg gagctgacat ctaccaacaa gatcaccaaa tggtgggaaa tgaccaccaa
     1681 catgaacttg ttcaattcac ggatcaagat tggtattgaa ggccagcccg aacaggatcc
     1741 gctgaccagc tggtttggca aactgaataa caactttaag ctggcaaaaa cacttacgct
     1801 gcaggtatcc ggagaatacc agtccaaaac aatcctgccc cccagcagtg gccgtggtgg
     1861 tggacgtgga ggtggtatgt ttggcggtgg cggcatgtgg ggcatgacaa ccacttcgca
     1921 gggttacatc aagtctaact attttgtgga tgcaggcctg cgctttgaat tcctgaaaaa
     1981 caagcaggct tccatatcgc ttaatgtcaa tgatattttc agaacccgtc gtcagaatat
     2041 tatttctgaa tcaccactgt tttaccagga agtgtttcgc cgcagggatc cgcagattct
     2101 gcgccttaac ttcaactgga gattcggtaa gttcgatgca aacctgttca aaagaaagaa
     2161 taaccgtgat aatatgggag atggcactga aggaataggt atgagacctt aaaaacaatc
     2221 tgattatcgt ataaggtttt gcatatttgc aaaaccttat ttttttaccg
//
LOCUS       DAORLS010000416         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25619_length_2269_cov_1.868112, whole genome shotgun sequence.
ACCESSION   DAORLS010000416 DAORLS010000000
VERSION     DAORLS010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25619_length_2269_cov_1.868112"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>228)
                     /locus_tag="PK977_19195"
     CDS             complement(<1..>228)
                     /locus_tag="PK977_19195"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF20317.1"
                     /translation="LLEKGNFSAFHRIKSDECWHFYYGDPLEIFVLQQTGELTTVTLG
                     NEMDKGQLFQYVVPAGCWFASRPAPGSQYCFV"
     assembly_gap    229..266
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    609..660
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<661..1321)
                     /gene="ygiD"
                     /locus_tag="PK977_19200"
     CDS             complement(<661..1321)
                     /gene="ygiD"
                     /locus_tag="PK977_19200"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRF20318.1"
                     /translation="MQRKQFIYTIGGLITMGTLAGFKRYTDDLEEQEEKMPVLFIGHG
                     SPMNGIENNTFSSQWEKMGKELPAPKAVLVISAHWLTNGTHITAMDHPKTIHDFGGFP
                     QELFEVQYPAPGDALLAQQTKDLIKSTNVGLNHDWGLDHGAWTVVRRMYPDAKIPVLQ
                     LSIDYSKPAQYHYNLAKELAALRKKGVLIIGSGNMVHNLRMVAWDKLDTPGFGYDWAI
                     EM"
     gene            1449..>1886
                     /locus_tag="PK977_19205"
     CDS             1449..>1886
                     /locus_tag="PK977_19205"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF20319.1"
                     /translation="MRSVNLITLLFCSQLLHAQVLIKNAHVVDVEKKKILSGYTVLVS
                     GDKIISVDKDKAFKLPGSTQVIDAAGKYLVPGFTDAHIHFFQSGGLYARPDAIDLRKY
                     RPYAEEIQWVHNHMEDFLRRYLSVGITTVIDVGATNNFLRQRDS"
     assembly_gap    1887..1988
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1989..>2269
                     /locus_tag="PK977_19210"
     CDS             <1989..>2269
                     /locus_tag="PK977_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF20320.1"
                     /translation="EMLTEEGTRKSVQEQLKLNPDFIKIWYIVTDKDVEKGARKNLAL
                     VKAAIDEAHKNNKRVAVHATERITAQLAVEAGADFLVHSVDDEIVPDDFV"
BASE COUNT          565 a          459 c          477 g          576 t
ORIGIN      
        1 gacaaagcag tactgactgc cgggcgcagg gcgactggca aaccagcaac cggccggaac
       61 tacatattga aacaattgtc ctttgtccat ctcattaccc agtgttactg ttgttagttc
      121 tcccgtttgt tgtaaaacaa aaatctccag cggatcaccg tagtaaaaat gccagcactc
      181 atcacttttg atgcggtgaa aggcagagaa gtttcctttc tccagcaann nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnngtca ccaccaaagc gttcgggtaa tgcggctgct
      301 ctgatatact cagcactctt atacgtttgt ttgtaccagc cgccttcggg atgcggcagc
      361 aggttgtatt gctgaatcaa ttgctgatgt gataacatag actgaattac acagcaagat
      421 agttatcgcc ggttatccaa acttcactga tgtcatattg agcgaaccgg ccactaactg
      481 gtcgttgaaa aaagaagggg tttcattttt ctcctgcaaa cccagtatat acatcagcgg
      541 gtaataatga tccggtgtgg gaatggccag ctttgctgat ttgctcaaag attcataatt
      601 gatcagtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 gcatttcgat ggcccagtcg taaccaaagc ccggcgtatc taatttatcc catgccacca
      721 tgcggagatt gtgcaccata ttgccgctgc caatgatcag tacacctttt ttacgcagcg
      781 ctgccagttc tttggcgaga ttgtaatgat actgtgctgg cttgctgtaa tcaatactta
      841 gctgcagtac aggaattttt gcatccgggt acattctcct caccacagtc catgcgccgt
      901 gatccaatcc ccagtcgtgg ttcaaaccta cattggtgga tttaatgaga tctttcgttt
      961 gctgtgccag caatgcatca ccgggggctg ggtattgcac ctcaaacagt tcctgcggaa
     1021 aaccaccaaa atcatgaatg gtttttgggt gatccatggc tgtgatatgc gtaccattgg
     1081 tgagccagtg ggcagagatg accagtactg ctttgggcgc aggcagttcc tttcccatct
     1141 tttcccattg tgaactgaat gtattgtttt caatcccgtt catcggtgaa ccatgaccaa
     1201 taaaaagtac aggcattttt tcttcctgtt cttccaggtc atcggtgtat cgtttaaaac
     1261 cagctaaagt tcccatggta atgagccctc cgattgtata aataaattgt ttgcgctgca
     1321 taaagcaggt taaaggtaca agattattat ttcaatgttg taacattaaa gcaaagcttc
     1381 tcttaaagtt ggcacttctg tttcccaatc attgtaaata agtaacttac ctgaataaag
     1441 ctatacttat gcgatcagta aaccttatta cccttttgtt ttgttcccag ctgctgcatg
     1501 cacaggtcct catcaagaat gcccatgtgg tagatgtgga gaagaagaaa atactcagcg
     1561 gttatacggt actggtgtcg ggagataaga tcatctccgt ggataaggac aaagcattta
     1621 aactgccggg tagtacacag gtgattgatg ctgccggcaa gtatttagtg cccggcttca
     1681 ctgatgccca tatacatttc tttcaaagcg gcggattgta tgcaagacct gatgcgatag
     1741 acctgcgcaa ataccggccc tatgcagaag agatccaatg ggtgcataac catatggaag
     1801 attttctgcg ccgttattta tcagtcggca ttacaacggt gattgatgta ggcgctacta
     1861 ataatttttt gcggcagcgg gattctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnga aatgctgaca gaagaaggaa cccgtaaatc ggtacaggaa caattgaaac
     2041 tgaatcctga ttttatcaag atatggtata tcgttaccga taaagatgta gagaaaggtg
     2101 cccgaaaaaa cctggcactg gtaaaagccg ccattgatga agcacataaa aacaataaac
     2161 gggtggcggt gcatgccacc gaacgcatca cggcgcaact tgccgtggaa gccggcgccg
     2221 attttttggt acacagtgta gatgatgaaa tagtgccgga tgattttgt
//
LOCUS       DAORLS010000417         2262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25726_length_2262_cov_2.037608, whole genome shotgun sequence.
ACCESSION   DAORLS010000417 DAORLS010000000
VERSION     DAORLS010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25726_length_2262_cov_2.037608"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PK977_19215"
     CDS             complement(<1..694)
                     /locus_tag="PK977_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRF20321.1"
                     /translation="MSNSLTIRQIELYKLSIPLIEPFTTSLGTDYSADNVLVKIITEE
                     GITGFGECSPYMPINGESQDTCFIVGQYFAKALKGKNALDIEGSIQLMDSIIYASTSI
                     KSAFDIALHDIAAQQAGMPLYKFIGGENNKTIVTDYTVSVGDIDKMVQEALRIQAQGY
                     PAIKVKLGKGGKQDVERIRAIRAAVGKEIPIRIDANQGWKVKEAIETLNALAEFDIQH
                     CEEPIARWKFMQL"
     gene            1125..1655
                     /locus_tag="PK977_19220"
     CDS             1125..1655
                     /locus_tag="PK977_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20322.1"
                     /translation="MKNILAAALLIVLFASCKKEVSDLPPETQTGANTFGAKVNGETW
                     VPQGFGPFPANNLLEARRIGGNVIINARNFSKSPRETEFEFFLKNVYSTGVYNLNTTS
                     GYPTQNSSYGYYVKRNLTPTNEFMTSATHTGTVTITRLDTINFVISGTFQFTAADIYG
                     SDPPITVSEGRFDIDF"
     gene            complement(1769..>2262)
                     /locus_tag="PK977_19225"
     CDS             complement(1769..>2262)
                     /locus_tag="PK977_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20323.1"
                     /translation="KDGIYVWFAYYTNGKFTNELTAAAKSPLTTPQQINYSNRATMRF
                     KHFSVGWRKYLKGNCEIEKGWNLYATAGFGLLPGRIINSHSVSIDTSLYHVPVREGKA
                     NFKRLTADLGLGWEKPVGADFFLYAEGRVWIPASDYPSKYIFVNDNAPLAAMMNVGLR
                     ILF"
BASE COUNT          577 a          486 c          539 g          660 t
ORIGIN      
        1 gcagctgcat gaacttccag cgggcaatag gttcttcgca atgctgtatg tcaaattccg
       61 ccagtgcatt taatgtttca atggcttctt ttactttcca gccctggttg gcatctatgc
      121 ggatgggaat ttcttttcct actgctgccc ggatggccct tattctttct acatcctgct
      181 ttccgccttt gcccagtttt actttgatgg cgggataacc ctgtgcctgt atgcgcaaag
      241 cttcctgtac cattttatca atatcgccca cactgacggt gtaatcggta acgatggtct
      301 tgttgttttc gccgccgatg aacttatata aaggcatgcc ggcctgttgt gctgcaatat
      361 catggagggc aatatcaaag gcactcttga tgctggtgct ggcatagatg atactgtcca
      421 tgagttgtat gctgccttca atatctaatg cgttttttcc tttgagtgct ttggcaaaat
      481 attgtcctac gatgaagcag gtgtcctggc tttcaccatt gatgggcata tagggactgc
      541 attcaccaaa accggtgatg ccttcttcgg tgatgatctt taccagcaca ttgtcggcgg
      601 agtaatcagt acccagcgat gtggtgaagg gctctatgag gggaatagag agtttgtata
      661 attctatttg acgtatagta agagagttgc tcataaacag ttggtatagg ggataaagat
      721 aaaatgtttg ctgcggatgc ggtggtgaaa aagaatggcc acgaaagatg gattgcctgt
      781 ggcaaaagtt ggttagccac ggattacacg gattaacacg gattgattgc ctttggcaat
      841 attttctagc gacgaatgaa atttattgaa tacaaattga ctgccttcgg caggttgttt
      901 ggccacagat gaacacaaat aaacacagat ttttttgcct tcggcaggtt attggtacac
      961 agatggacac aaataagcac agatgaatag aggtttattg cgtcttagcg tctttgcgtg
     1021 aaattatctt agtgcccttg gtgcctttgc gggaaatatt ctggccttgt ttttgtcctt
     1081 atacgaaagt caccgaaaaa gcgttataga acgaatccat gtatatgaag aatatccttg
     1141 ccgccgcttt attaattgtg ctttttgcat cctgtaaaaa agaagtatct gacctgccac
     1201 ctgaaacaca gaccggcgcc aacacattcg gcgctaaggt gaatggtgaa acctgggtgc
     1261 cgcagggctt tggccccttc ccggccaata acctgctgga ggcaagaagg atcggcggca
     1321 atgtgatcat caatgcaagg aatttttcca agtcgccaag ggagacggag tttgaatttt
     1381 tcctgaagaa tgtatacagc acgggggtgt ataacctgaa taccacatcc ggctatccta
     1441 cacagaacag cagttatggt tattatgtaa aaagaaacct gacgcctacc aatgagttca
     1501 tgacctctgc cacacatacc ggcacggtga cgattaccag gttagatacg atcaattttg
     1561 ttatttccgg tacgttccag tttaccgctg cagatattta tggctctgac ccgcctatta
     1621 ctgtgagtga aggaaggttt gatattgatt tttgataatg tagttgtcat gctgagcctg
     1681 tcgaagcatg aatatgatgt cggttttcac ccttcggccc ttcgttacct cagggtaaac
     1741 tgagctcagg gtgacagaag ccctcttttc agaaaagaat acgcagcccc acattcatca
     1801 tggctgccag cggggcatta tcatttacaa agatgtattt gctgggataa tcagaagcag
     1861 gaatccatac cctgccttca gcatagagaa agaaatcagc accaacaggt ttttcccatc
     1921 cgagaccgag atctgcggtg agccgtttga aattggcttt gccttcccgt accggcacat
     1981 ggtataaact ggtgtcaatg cttaccgaat gtgaattgat gatgcggccg ggcaataaac
     2041 caaaaccggc agtggcataa aggttccagc ctttttcaat ttcacaattt cctttcaggt
     2101 actttctcca gcccaccgaa aaatgtttga accgcatagt ggccctgttg ctgtaattta
     2161 tttgctgtgg tgtggtgagt ggtgatttgg cagctgctgt cagttcgttg gtaaactttc
     2221 cgttggtgta ataagcaaac cagacataga taccgtcttt cg
//
LOCUS       DAORLS010000418         2262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25727_length_2262_cov_1.949252, whole genome shotgun sequence.
ACCESSION   DAORLS010000418 DAORLS010000000
VERSION     DAORLS010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25727_length_2262_cov_1.949252"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..>342)
                     /locus_tag="PK977_19230"
     CDS             complement(153..>342)
                     /locus_tag="PK977_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRF20324.1"
                     /translation="MKVMAIVTCLLAPATVIGGIFGMNFDTNVNFFHQRWGFYVAVGM
                     MLVIPVGMLWAFRKRGWF"
     assembly_gap    343..397
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<398..1264)
                     /locus_tag="PK977_19235"
     CDS             complement(<398..1264)
                     /locus_tag="PK977_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRF20325.1"
                     /translation="MNVKLRPEKYLRYLYLPNLFATKRTKEILSVNPTVEPHREEAKE
                     VHVHIFDYDAEHLEEKKLTSVKDCFQYKTSGRTTWINVDGLRKADVEAICSNYDIHPL
                     VIEDILSINQRPKLDEADNSIFCLLNMLYYNQETHAVEQEQISIILAKDVVISFQEDA
                     HRDVFFSLRDKLRITNGKVRQRTADFLCYILLDMIVDHYFVVMEKLGERIEEVEEEVM
                     HGKNPRALAHITQLRKELIVLKRNFAPVREVVNGFLRSESELLEDRHTKYYKDVYDHI
                     VQAIDLVENYRDV"
     gene            complement(1414..>2262)
                     /locus_tag="PK977_19240"
     CDS             complement(1414..>2262)
                     /locus_tag="PK977_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF20326.1"
                     /translation="NHVLNESPGLLRDVFYLENGTTTISSGQNKNAGLRIKAGNETEL
                     LYRYQLNDIGWMGDADTALRKHKLLLLQTAVKDNPGSFFLLQSIADAKELYSKEELQY
                     LLSLFDAKVLSSAAGKKISDYLRLRPDAGMAYPKLWLPGVADTLEPLIDPTAKLNVLV
                     FWASWCGPCIKEIPQLKQLQERFGKNGLRMTSVSIDTNKEAWQEAVLQHAMPWRQLLI
                     DTDKIEELQQIFGFTTIPFLVMTDSEGKEVARFADYDEGNTKKYAEVIERFLFSRKGA
                     IRIVSH"
BASE COUNT          544 a          568 c          449 g          646 t
ORIGIN      
        1 aatttcaaca acaattttct tttccgtcag atcagaatat ttatatccgg gagaccgcat
       61 gcggtttgcg ataatagaat ctgcttttca aaaaaagaat ttttctcaag agaacatctt
      121 cattataatc attcaatcat aagaatccgt ggtcagaacc accccctctt tctaaacgcc
      181 caaagcatac caacaggaat aaccagcatc atgccgacgg caacataaaa gccccagcgc
      241 tggtgaaaga agttaacatt agtatcaaag ttcatcccga agatgccccc gattacggta
      301 gctggggcca gtaagcaggt aacgatggcc attaccttca tcnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac atcacggtag ttctctacca
      421 ggtcgatagc ctgtacaatg tggtcataaa cgtctttata gtatttggta tggcggtctt
      481 ccagtagttc actttcgctg cgcaggaaac cattcaccac ttcccttacc ggtgcaaaat
      541 ttcgcttcag tacgatcagc tctttgcgaa gttgtgtgat atgggcaagg gcccttgggt
      601 ttttgccatg catcacctct tcttctacct cctcaatacg ttcacccagt ttttccatca
      661 ccacgaagta gtggtcaacg atcatatcga gcagtatata acaaagaaaa tcggccgtac
      721 gctgccgcac tttaccattg gtgatgcgca gcttgtcccg caaagaaaaa aacacatccc
      781 ggtgtgcatc ctcctggaaa ctgatcacca catctttcgc cagtataata ctgatctgtt
      841 cctgctccac cgcatgggtt tcctggttat agtacagcat attcagcagg cagaagatgc
      901 tgttgtctgc ttcatccagt ttaggccgct ggttgatgct cagtatatct tcaataacga
      961 gtgggtgaat atcatagttc gaacagatag cttccacatc tgctttgcgc aacccgtcca
     1021 cattgatcca ggtagtgcgg ccgctggttt tgtattgaaa gcaatctttt acacttgtca
     1081 gctttttttc ttccaggtgc tctgcatcat aatcaaaaat atgtacatgc acttccttcg
     1141 cttcttcccg gtgcggctct accgtggggt ttaccgataa gatctctttc gttctctttg
     1201 tggcaaaaag attcggcagg tagagatacc gtaaatattt ctcggggcga agctttacgt
     1261 tcataacagg gaagttagaa aaattactct ttcccacaaa tacgttttat ggttcccgct
     1321 aagacactaa gaacacaaaa taataagctg ctggctgcct tgttggcaac cctcatttgt
     1381 ttgttctaaa atattctttg cgccctttgt gtcttagtgg gaaacaattc ttattgcgcc
     1441 tttgcgtgaa aacaaaaacc gttcaatcac ttccgcatac ttcttcgtat tcccctcatc
     1501 ataatctgca aaccttgcta cttcttttcc ttcgctgtcg gtcattacca ggaatggtat
     1561 cgttgtaaag ccgaagatct gctgcagttc ttctatcttg tctgtatcaa tcagcagttg
     1621 ccgccacggc atggcatgtt gcagcaccgc ttcctgccag gcttctttat tcgtatcaat
     1681 agaaacactg gtcatgcgca aaccattttt gccaaatctt tcctgcagtt gtttcagttg
     1741 tggtatttct tttatacagg gtccgcacca gctggcccag aacaccagca cattcagttt
     1801 cgctgtcggg tcgatcagcg gctccagtgt gtccgccacc ccgggcaacc acaacttcgg
     1861 gtaagccata ccggcatcgg ggcgaagccg caggtagtca ctgatttttt ttccggctgc
     1921 agatgacaac accttcgcat caaagagcga caacaggtac tgcaactcct ccttgctgta
     1981 tagttctttt gcatctgcaa tgctttgcag taaaaagaag gagcccgggt tgtcttttac
     2041 cgctgtttgc agcaacaaca gtttatgttt tcgcagtgcg gtatccgcat cgcccatcca
     2101 gccaatatca ttcagctggt agcggtacag cagctctgtt tcattgcccg cctttatacg
     2161 aaggccggca tttttatttt gaccactgct gatagtggtt gttccgttct ccaggtaaaa
     2221 cacatcccgc agcaggcccg gactttcgtt cagtacatgg tt
//
LOCUS       DAORLS010000419         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_25940_length_2248_cov_1.693570, whole genome shotgun sequence.
ACCESSION   DAORLS010000419 DAORLS010000000
VERSION     DAORLS010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25940_length_2248_cov_1.693570"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>263)
                     /locus_tag="PK977_19245"
     CDS             complement(<1..>263)
                     /locus_tag="PK977_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRF20327.1"
                     /translation="INPAFIKAHTTELIILFIVFLAETGFAFYRYGKMTNFHTYLAKL
                     AALLQGVFLLVAIVFNEPNLILFYAAAGITLIELVEEIIITAV"
     assembly_gap    264..291
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<292..528)
                     /locus_tag="PK977_19250"
     CDS             complement(<292..528)
                     /locus_tag="PK977_19250"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRF20328.1"
                     /translation="MRTHKQAWYIINGITLYRVIAAPFLLVLLFNGYYQLFKWLLAVS
                     FFTDLIDGYLARKFKVTSVMGTRLDSIGDDLTVLT"
     gene            complement(515..895)
                     /locus_tag="PK977_19255"
     CDS             complement(515..895)
                     /locus_tag="PK977_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20329.1"
                     /translation="MRRVLCILIVLLVALLHIIGPGKWFHGSYRDLYYSYFSDVAIPF
                     AFYFLLSIAERNWPLVSGWKMKATLILGGCVLAESLQYFHVHFLGTVFDPLDVIAYII
                     GIAAAIVVDELLLQRLKKSGHANA"
     gene            complement(997..>1582)
                     /locus_tag="PK977_19260"
     CDS             complement(997..>1582)
                     /locus_tag="PK977_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20330.1"
                     /translation="DDNDFWRSFLNVKELPEEIRLQAEKVYGLPDKTVSMQAFFDLLA
                     IHELGHAFHMQGKLQVQRKWMGELFCNILLHTYIAEKEPEQLPALTLFPRIVVNGGVK
                     NYRFTSLTDLEERYDEIGQQHPQNYGWYQCRWHAAAADIYDADGKVVMQRLWNTLKSN
                     QEKMNDTDFAESLHNHVSKTVADVLRRWEADMIR"
     assembly_gap    1583..1599
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1600..1893)
                     /locus_tag="PK977_19265"
     CDS             complement(<1600..1893)
                     /locus_tag="PK977_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20331.1"
                     /translation="MKPVMLFICATLFRLTSFAQPYDKLQSLSGAGVTTFYSEGFKSR
                     AQQTATRLEKVMTYYQEMLGFKPVVTVLLLNKADWPLYTKFPVYGMPHYNDDAT"
     assembly_gap    1922..1969
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1970..2187)
                     /locus_tag="PK977_19270"
     CDS             complement(<1970..2187)
                     /locus_tag="PK977_19270"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF20332.1"
                     /translation="MGMSPKLFARIVRFSKAYRMREQFLQMSWTKIAYECGYFDQMHF
                     IRDFKEFAGVTPRILEKELAQLPIRLQGP"
BASE COUNT          624 a          508 c          449 g          574 t
ORIGIN      
        1 aatacggctg taataattat ttcttcaacc agttcaatta aggtgatgcc ggctgcggcg
       61 taaaacaata taaggtttgg ttcattaaaa acaatggcca ctaataaaaa aacaccctgc
      121 aacaaagcag ccagtttggc caggtaggta tgaaaattgg tcatcttgcc ataccggtaa
      181 aaagcaaaac cggtttccgc aagaaataca ataaagagaa tgatcagttc ggtagtatgc
      241 gcctttataa aggcaggatt tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtcagcac
      301 ggtcaggtca tcaccgatag aatccagccg ggtacccatt acactggtta ctttgaattt
      361 ccgggccagg taaccatcta tcaggtcggt aaaaaagctg acggccagta accatttaaa
      421 gagctggtaa tatccattaa acagcaatac aagtagaaat ggcgcagcta tcacccggta
      481 aagagtgata ccgttgataa tataccatgc ctgtttatgc gttcgcatgg ccggattttt
      541 ttaatcgttg taataataac tcgtcaacaa caatggctgc cgcaatgcca attatataag
      601 caataacatc cagcggatca aatacagttc ccaggaaatg aacatgaaag tattgcagtg
      661 attcggctaa aacacaaccg cccaatatta gggtggcctt catcttccag ccggatacca
      721 ggggccagtt tctttctgcg atactcaata aaaagtaaaa agcaaaaggg atagcaacgt
      781 ctgaaaaata actgtaataa agatccctgt atgatccatg aaaccatttg cctggcccga
      841 ttatatgaag caacgctacc agtaatacaa tgaggatgca aagaaccctt cgcataccag
      901 caagttacaa aataattacc cgctcttatt aagctgtaat gtgtactgct cttgaacaga
      961 gcggcaccct ttgtttattg ctgctatcta ttatcctcat cttatcatat cagcttccca
     1021 ccttctcagc acatcggcca cggtcttgct tacatggtta tgcagcgact ctgcaaaatc
     1081 cgtatcgttc attttttctt gatttgattt taatgtattc cacaatcgct gcatcactac
     1141 ttttccatct gcatcataaa tatcggcggc agctgcatgc cagcggcact ggtaccagcc
     1201 atagttctgt ggatgctgct gaccgatttc atcatatcgc tcttccaggt cagttaaact
     1261 ggtgaagcga taatttttta cgccaccatt cactacgatg cggggaaaca gtgtaagagc
     1321 cggcaactgc tccggttctt tttctgcgat ataagtatgc agcaggatat tacagaacaa
     1381 ctctcccatc catttgcgct gcacctgtaa tttgccctgc atatgaaacg catggcccag
     1441 ttcatgtatg gccagcaggt caaagaaagc ctgcatacta acggtcttat ccggcaggcc
     1501 atatactttc tccgcctgca gccgaatctc ttccggcagt tcttttacat tcaaaaaact
     1561 tctccagaaa tcattgtcgt cgnnnnnnnn nnnnnnnnng gtagcatcat cattataatg
     1621 cggcatacca tacaccggga atttagtgta cagcggccag tcagccttgt ttagcagcag
     1681 caccgtcact accggtttga agcccagcat ctcctggtaa taggtcatta ccttttccag
     1741 ccttgtagca gtttgctgcg ctcttgactt aaagccttca ctgtaaaaag tagttacccc
     1801 ggcaccgctt agcgattgca gcttgtcata gggctgagca aaacttgtta agcggaaaag
     1861 agtggcgcag ataaaaagca ttacaggttt catgatattt cattttacac aaacctctgc
     1921 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggccctgcag
     1981 gcggatgggc agttgggcca gctccttttc caggatgcga ggcgttaccc cggcaaattc
     2041 tttaaagtcg cggataaaat gcatctggtc gaaataaccg cattcgtatg caatctttgt
     2101 ccagctcatt tgtaaaaatt gttcccgcat acgataggct tttgaaaaac gaacaatccg
     2161 ggcaaataat ttaggtgaca tgccaatgcg ttgatgacag gtacgttcaa actggcggag
     2221 gctcaggcag gccagtgaag ccagtttt
//
LOCUS       DAORLS010000420         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_26006_length_2243_cov_2.175960, whole genome shotgun sequence.
ACCESSION   DAORLS010000420 DAORLS010000000
VERSION     DAORLS010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26006_length_2243_cov_2.175960"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PK977_19275"
     CDS             complement(<1..229)
                     /locus_tag="PK977_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF20333.1"
                     /translation="MPKDIFTDQPTLENERVILRPIRSDDAEHLLPFSLHEPEIWKYG
                     LVTAAGKENLESYVHNAVKNLQEKKEYPFIVF"
     gene            complement(587..1189)
                     /locus_tag="PK977_19280"
     CDS             complement(587..1189)
                     /locus_tag="PK977_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20334.1"
                     /translation="MKHFLLIIFTALIVSGSRGQSAEYYANGYLLYGKDTTWCKIWFN
                     PSSPFYKFSVTTWQKDTAVEIPFTDTKLTGFGITDNDYNLHYGRIIVDGKKGKTNMHV
                     LKIVAGTLELYEHHYVLVATVRGTLNTTRKDYFNYYLGRTDLDPASNPVKLPALKKKF
                     ILPFISDNEKLCDELQDKLTPEELAAIVIRYNKEKYAATH"
     gene            complement(1186..>2243)
                     /locus_tag="PK977_19285"
     CDS             complement(1186..>2243)
                     /locus_tag="PK977_19285"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRF20335.1"
                     /translation="SEAYKKKSGRLRGSVMLAGPMLARFRKAFIPKPGGDKIGRRRLD
                     THIIGFEKLGASFIYHEEDGFFHLDAPDLKGATMLLDEPSVTGTANIVMAASMAKGTT
                     TIYHAACEPYIQQLCRMLNRMGARISGIGSNMLTIEGVDYLGGTTHRMLPDMIEVGSF
                     IGLAAMTQSDITIKGAGIEHLGMIPEKFQQLGIQMNFSGDDIHIPSQDSYEIQTFLDG
                     SVMTIYDHPWPGFTPDLLSIVLVTAIQAKGSVLIHQKMFESRLFFVDKLIDMGARIVL
                     CDPHRAVVIGLEREQALRGITMSSPDIRAGVALLIAALSAEGKSIIQNIEQIDRGYQD
                     IDGRLRMLGADIKRIQE"
BASE COUNT          570 a          477 c          520 g          676 t
ORIGIN      
        1 caaaaacgat aaagggatat tcttttttct cctgtaaatt tttaacggcg ttgtgtacat
       61 agctctccag gttttctttg ccagctgctg ttaccaatcc gtatttccat atttccggtt
      121 cgtgtaacga aaaaggaagc aggtgctccg catcatcact gcggatgggc cgcaggatga
      181 ctctttcgtt ttcgagagtg ggctgatcgg tgaaaatatc tttgggcatg tttggttttt
      241 gtaaagataa tttttgcctc ttgtttactt tttttcttga caaaaaaagt aagaaaaaag
      301 tcaagtcccg gcgaatgctc cgccgccggg accgcctgcc cacacggaga tgctgatggt
      361 ctgcaaaaac attaaatgtt tgagataagt agttctggtc tccgcttcgt gttatgcaat
      421 gttcaggaag gtgcagtgtt ccgccttgct tttctgtaag tgtttagata ccacaaccaa
      481 ggcggaacaa ggcgctggaa aatttattaa agaatgaaac aggtactgag cgttattctt
      541 gtattctatc tgttatacaa agtgagaatt ttgttatact ccattatcaa tgcgttgctg
      601 catatttttc tttgttatag cggatgacga tggcagctag ttcttccggg gttaatttat
      661 cttgtagttc atcacagagt ttttcattgt cactgataaa cgggaggatg aattttttct
      721 ttaatgccgg tagtttgacc gggtttgaag ccgggtcaag atctgttctg cccaggtagt
      781 aattgaagta atcttttcgg gttgtattta atgtgccacg aacggtggct accagtacat
      841 aatgatgttc atatagttct aatgtaccgg ccactatttt cagtacatgc atattggttt
      901 tacctttttt accatccact attatcctgc cgtaatggag gttgtagtca ttgtcggtaa
      961 tgccgaagcc ggtaagtttg gtatcagtaa atggtatttc gacagctgta tctttttgcc
     1021 aggtggtgac ggaaaattta taaaaaggag atgaaggatt gaaccagatt ttacaccatg
     1081 tagtgtcttt gccataaagc aaataaccat ttgcatagta ttcagcagac tggccccggc
     1141 tgccagaaac aattagtgct gtaaaaatga taaggaggaa atgcttcatt cctgtatccg
     1201 ttttatatct gcgcccagca ttcgcagcct gccgtcaata tcctggtagc cgcggtcgat
     1261 ctgttctatg ttttgaatga tgcttttgcc ttctgcactt aatgctgcaa ttaataaagc
     1321 tacaccggca cggatatccg gcgaactcat ggtgatgccc cgcaaggcct gttctctttc
     1381 gaggccgatc actacagccc ggtgcggatc gcagagaaca attcttgcac ccatatcaat
     1441 cagcttgtca acaaagaaca aacggctttc aaacattttc tggtgaatga gcaccgagcc
     1501 tttggcctgt atagcagtaa ccaatacaat gcttaataaa tcaggtgtga agcccggcca
     1561 gggatgatcg tagatggtca tgaccgagcc atcgagaaaa gtttgtattt cataactatc
     1621 ctgcgaggga atatgaatat catcacccga aaagttcatt tgaatcccga gctgctgaaa
     1681 tttttcgggt atcatgccga ggtgttcgat accggcgcct ttgatagtga tatcactttg
     1741 cgtcatagcc gccaaaccga tgaaagaacc tacttcgatc atatcgggca gcatgcgatg
     1801 ggtagtgccg ccgagataat ctactccttc aatggtcagc atattgctgc cgatgccact
     1861 gattctggcg cccatgcggt tcagcatgcg gcagagctgt tgtatataag gctcgcaggc
     1921 ggcatgatag atggtggtgg ttcctttcgc catcgaagca gccattacga tattggcagt
     1981 accggtaacg gatggttcgt cgagcagcat agttgcgcct ttcagatcag gtgcatcgag
     2041 atgaaagaaa ccatcttctt catgataaat gaaactggcg ccgagttttt caaagccgat
     2101 aatatgagta tctaatcgtc gcctgcctat tttatcacca ccgggttttg gtataaatgc
     2161 tttgcgaaac cttgccagca tggggccggc cagcattaca gagccacgca gccttccgct
     2221 tttcttttta taagcttcgc tgt
//
LOCUS       DAORLS010000421         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_26009_length_2243_cov_1.702925, whole genome shotgun sequence.
ACCESSION   DAORLS010000421 DAORLS010000000
VERSION     DAORLS010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26009_length_2243_cov_1.702925"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PK977_19290"
     CDS             <1..559
                     /locus_tag="PK977_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF20336.1"
                     /translation="KQIRIAETEKYPHVSFFFSGGREVPFEGEKRIMIPSPKVATYDL
                     QPEMSAYQVTEALLPEIKNKTADFICLNYANADMVGHTGVWQAAIKAVETVDKCVEQI
                     VTAGLENGYTIFLTADHGNSDYMINEDGSPNTAHTLNPVPFFIIDKEWKGTIKPGKLG
                     DIAPTILTMMELPVPAEMTGNILIN"
     gene            591..1076
                     /locus_tag="PK977_19295"
     CDS             591..1076
                     /locus_tag="PK977_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HRF20337.1"
                     /translation="MIKKILLVLLAALIVIQFIHPKKNKSEGPQPNYIGNIFNIPEDV
                     KAILAKACNDCHSNNTRYPWYANLQPVHWWLENHIKEGKKKINYDEFTHRPLRYQYHK
                     MEETIELVKEGEMPLNSYTWTHKDAKLTDEEKSKLTGWAQSVMDTMKARYPIDSLVRK
                     K"
     gene            1225..1617
                     /locus_tag="PK977_19300"
     CDS             1225..1617
                     /locus_tag="PK977_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20338.1"
                     /translation="MEKSLLHTIRIDEDKVVWIKPGKEIFVNGKMFDIKKTEKTNGKV
                     IFHGLYDEEETALKKNIENSMKKDRSSQQYLLSNLFSILFGAYHQSGDTEVPVLILNA
                     EKNGIIIHPWLQPVLTILTPPPRNMMIA"
     gene            1654..>2243
                     /locus_tag="PK977_19305"
     CDS             1654..>2243
                     /locus_tag="PK977_19305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF20339.1"
                     /translation="MRKIALLLALLYMSICLHAQEDTTQEKNLDEIVVYSNKFVEKKK
                     NLAQKIDVVTARTISRFNAQNTGDLLINTGNVFVQKSQQGGSSPVLRGFEASRVLLVV
                     DGVRMNNAVYRAGHLQNAITVDQNMLERVEVLYGPSSTIYGSDALGGVIHFRTKSPQL
                     STNQKLLVKGSSFIRYSTANEEKTGHADVSVGGKRLG"
BASE COUNT          762 a          437 c          464 g          580 t
ORIGIN      
        1 gaagcagatc cgtattgcag aaacggagaa atatccgcat gttagtttct ttttcagcgg
       61 tggccgtgaa gtcccttttg aaggtgaaaa aaggatcatg atcccttctc ccaaagtagc
      121 cacctatgat ctgcaaccgg agatgagtgc ttaccaggtt acggaagcat tacttccgga
      181 gataaaaaac aaaaccgcag acttcatttg cctgaactat gccaatgctg atatggtagg
      241 gcacaccggt gtctggcagg cagctattaa agcagtcgaa acagttgata aatgtgtgga
      301 gcaaattgtg accgcaggtt tagaaaacgg ttatacgata tttctcactg cagaccatgg
      361 caactcggat tatatgatca atgaagatgg atcgcctaat acagcgcata cactgaaccc
      421 ggttcctttt ttcattattg ataaagaatg gaaaggaacc attaagcccg gtaaactagg
      481 cgatatagct cctactattt tgacaatgat ggagcttccc gttccggcag aaatgaccgg
      541 gaatattctg atcaattaat tttaatttta actgaaacaa ctaatagctt atgattaaaa
      601 agatattatt ggtacttctg gctgcactaa tcgtcattca gtttatacac ccaaagaaaa
      661 acaaaagtga aggccctcag cctaattaca taggcaacat cttcaatata cctgaagatg
      721 taaaagctat actggctaaa gcctgcaatg attgtcattc caataatacc cgctacccat
      781 ggtatgccaa cctgcagccg gtacattggt ggctggaaaa tcatattaaa gaaggcaaaa
      841 agaaaattaa ttatgatgaa ttcacccaca ggccccttcg ttatcaatac cacaaaatgg
      901 aagagacaat agaattggta aaagaaggcg agatgccttt aaattcttat acctggacac
      961 acaaggatgc aaaattaacc gatgaagaaa aatctaaact gaccggatgg gcccaatctg
     1021 ttatggatac catgaaagcc cgatacccga tagatagcct tgttcgtaag aaataatgat
     1081 attttaggtg tcataatttc tgtgaaaacc aaccaacata aaatactcag cctttttttc
     1141 atcctgcttg ggacgctgcc gtttattttt tcaatcactg tcattctcaa aaaacaacag
     1201 atacgccatg aaatgaagga gcgaatggag aaatcccttc tgcataccat taggatagat
     1261 gaggacaaag tcgtctggat aaagcctggt aaagaaattt ttgtcaatgg taaaatgttc
     1321 gacataaaaa agacagagaa aacaaacggt aaggttatat ttcatggact gtatgatgag
     1381 gaagaaacag cattgaagaa gaatattgaa aacagcatga aaaaagaccg gtcatcacag
     1441 caatatcttc tttctaatct tttctcgata ttatttggag cctatcacca atccggcgat
     1501 actgaagtgc ctgttttaat tttaaatgca gaaaaaaacg gcattattat tcacccttgg
     1561 ttacaaccag ttttgacgat attgaccccg ccgccgcgca acatgatgat agcataattc
     1621 atactaacaa cttcttatca tcaaattcag tttatgagaa aaattgcatt gctgctggca
     1681 ttgctgtata tgtccatatg cctgcatgca caggaagaca caacacaaga aaaaaatctg
     1741 gacgaaatag tagtttactc aaataaattc gttgaaaaga aaaagaatct tgcacaaaag
     1801 attgatgtag ttactgcaag gacaatttcc aggttcaacg cacaaaatac cggcgacctg
     1861 ttgataaata ccggtaatgt atttgtacaa aaaagccagc aaggtggtag cagcccggta
     1921 ctaagaggat tcgaggcaag cagggtgctg ctggtcgttg atggagtaag aatgaacaat
     1981 gctgtttatc gggccggaca cctgcaaaac gccattacag tggatcagaa tatgctggaa
     2041 cgggttgaag tgctttacgg tccttcatct acgatttatg gtagtgatgc cctgggtggt
     2101 gtaatccatt tccgcaccaa atcaccacaa ctgagtacca atcaaaaatt attggtgaaa
     2161 ggatcatctt ttatacggta tagcactgcc aatgaagaaa aaaccggtca tgcagatgtg
     2221 agtgtaggcg ggaaaagact ggg
//
LOCUS       DAORLS010000422         2237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_26094_length_2237_cov_1.889093, whole genome shotgun sequence.
ACCESSION   DAORLS010000422 DAORLS010000000
VERSION     DAORLS010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26094_length_2237_cov_1.889093"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK977_19310"
     CDS             <1..435
                     /locus_tag="PK977_19310"
                     /inference="COORDINATES: protein motif:HMM:NF014892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRAM domain-containing protein"
                     /protein_id="HRF20340.1"
                     /translation="SSVTAGLIAGLISGVLFGWLIGLFANSKFVKQSTKIETGPDESI
                     VFETPANHFKGIEGVGGKLYLTNKRLVFKSHNLNIQNHQLSILLTDIKNVGRHKTLGL
                     ANNGLSIVTAEDKTEKFVVEQAENWIKHLTEKMESPALREFS"
     gene            623..805
                     /locus_tag="PK977_19315"
     CDS             623..805
                     /locus_tag="PK977_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20341.1"
                     /translation="MTTLPCSIQYKALKTIPLIAKNTQPELVVKDPGLAVKRPGFTVK
                     DPEPAVKGPEAMVKDP"
     gene            872..1414
                     /locus_tag="PK977_19320"
     CDS             872..1414
                     /locus_tag="PK977_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20342.1"
                     /translation="MVKGTGLFVKHPGFTVKHPECMVKDIWLVINQPASASSTPVKNF
                     LPSLSQHQYTALSATFNKPVAAGMLSGNTKLIIIKIIHTLVWIFFNIVIFYLLYAAIT
                     NKIDKWLWIGYGLFVLEGIVLLCFRFFCPLTVIARKYSNSTKHNFDIYLPEWLAKHNK
                     LIYTSILGVAILITIYQLVK"
     gene            1444..>2237
                     /locus_tag="PK977_19325"
     CDS             1444..>2237
                     /locus_tag="PK977_19325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF20343.1"
                     /translation="MSNTEKIKALRKELHRHPELSGQESSTAQRIINFAQQYQPTTII
                     SSIGGHGLAIVYEFSNDGPCIMLRCELDALPIEEANPFEHRSVVKGISHKCGHDGHMA
                     IVAGLMGWIEERSFTKGKIALLFQPAEETGQGAPAVLNDPSFAGIKPDYLFALHNLPG
                     EPVHQIIIVENRFSATVQSLVIHLQGKQSHASEPEKGINPALAMAFITKRFAELINDD
                     VKDESFALLTPVYSRMGAPAYGISAGEGELHYTIRTWSEEKMQLLK"
BASE COUNT          658 a          576 c          457 g          546 t
ORIGIN      
        1 agctccgtta ctgcaggact tatcgccggt ttaatatccg gggtattatt tggctggctg
       61 atcggcctct ttgcaaattc aaaatttgta aaacaatcaa cgaagattga aaccggaccg
      121 gacgagagta ttgtgtttga aactccggca aatcatttta aaggaattga aggcgttggc
      181 ggaaaattat acctcaccaa caagcggctg gtttttaaat cgcataactt aaatattcaa
      241 aaccaccagc tctccatact attgactgat ataaaaaatg taggcaggca caaaaccctg
      301 ggactagcga acaacgggct ttcaatagta actgctgagg ataaaactga aaagtttgta
      361 gttgaacagg cagaaaactg gattaaacat ttgacagaaa agatggaatc accagctctg
      421 cgagaatttt cctgagctgg tcgaagggtc tcacagccct attcgataac ctccggctat
      481 ccgtaaaaga aaaaaatccg gcttctctta gttgtacggg accggaataa atcatcccct
      541 caatcccaat aatcttccca aatccaggtt cagacaaaca cacagccgga ctgtccgcaa
      601 actaaaccgt tggccgttta aaatgacaac cctcccctgc tcaattcaat acaaagcact
      661 caaaaccatc cccttaatcg caaaaaacac ccaacccgaa ctcgttgtta aagaccccgg
      721 attggctgtt aaacgacccg gattcactgt caaagacccc gaaccggctg ttaaaggccc
      781 cgaagcaatg gttaaagacc cctgaatagc tgttaagcat cccgaacccg ttgttaaaga
      841 ccccgaaccg gctgttaaaa gccccggact catggttaag ggcaccgggc tgtttgttaa
      901 gcaccccgga ttcactgtta agcatcccga atgcatggtg aaagacatct ggctggtaat
      961 taatcaaccc gcatccgcat cctccacccc cgtcaaaaac ttcttacctt cattatctca
     1021 gcatcagtac actgctttat cagcaacttt taacaaacca gttgcggccg gtatgttatc
     1081 aggaaacacc aaacttatca tcatcaagat catccatacc ctcgtctgga tattcttcaa
     1141 tattgtcatt ttctatttac tgtatgccgc catcaccaat aaaatagata aatggctatg
     1201 gatcggctat ggcctttttg tactcgaggg catcgtactg ctttgcttca ggtttttttg
     1261 tcccttaacc gttattgcca gaaagtattc aaactccacc aaacacaatt ttgatattta
     1321 cctgcccgaa tggctggcaa aacataacaa actaatttac acttccatcc tgggggtggc
     1381 tatactgata acgatttatc agctggtaaa ataaaatatg ggtacaaaca gattcacatt
     1441 acagtgagta atacagaaaa aataaaagcc cttcgcaaag aactgcaccg gcatcccgaa
     1501 ctgtccgggc aggaatcgtc cacggcgcaa cgcatcatta attttgccca acaatatcaa
     1561 ccaaccacca tcatctcatc catcggcggc cacggtcttg ccattgtgta tgaattttcc
     1621 aacgatggcc cctgcatcat gctccgctgc gagctggatg cactgcccat cgaagaagcc
     1681 aatccctttg aacatcgttc tgtggtaaaa ggcatatcgc acaaatgcgg tcatgacggg
     1741 cacatggcca tcgtagcagg gttgatgggc tggattgaag aacgatcgtt taccaaagga
     1801 aaaattgcat tgctctttca gcctgccgaa gaaacaggtc agggcgcccc tgccgtactg
     1861 aatgatcctt cattcgccgg cattaaaccg gattatcttt ttgccttgca caatcttccc
     1921 ggcgagccgg tgcatcaaat tattattgta gaaaatcgtt tttctgccac cgtgcaaagc
     1981 ctcgtcattc acctgcaggg aaaacaatca catgcctccg agccggagaa aggcatcaac
     2041 ccggcgttgg cgatggcctt catcacaaag cgttttgccg agctgatcaa tgatgatgtg
     2101 aaagacgaat catttgcatt gctcaccccg gtgtacagcc gcatgggggc tccggcctat
     2161 ggtatcagcg ccggcgaggg cgaactgcat tataccatcc gtacctggtc ggaagaaaaa
     2221 atgcagctgc tgaaaga
//
LOCUS       DAORLS010000423         2230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_26192_length_2230_cov_2.197241, whole genome shotgun sequence.
ACCESSION   DAORLS010000423 DAORLS010000000
VERSION     DAORLS010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26192_length_2230_cov_2.197241"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>548
                     /locus_tag="PK977_19330"
     CDS             <1..>548
                     /locus_tag="PK977_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF20344.1"
                     /translation="FSPMVDICRDPRWGRIAEGSGEDAYLGSEIAKAMVKGYQGNDLT
                     KNNTIMSCVKHYALYGAAEAGRDYNTTDMSRQRMYNDYFPPYKAAVDAGVGTVMASFN
                     EIDGVPATANKWLMTDVLRKQWKFNGFVVTDYTGINEMIDHGIGDLQTVSARALDAGI
                     DMDMVGEGFLTTLQKSLKEKKI"
     assembly_gap    549..716
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <717..1843
                     /locus_tag="PK977_19335"
     CDS             <717..1843
                     /locus_tag="PK977_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRF20345.1"
                     /translation="SFVLLRNQNNVLPLKKSGTVALIGPLSDNKENMPGTWSVAADFS
                     KATSLLAGMKELVGDKVKILHARGSNLDEDSLFEERAGMFGKSLRRDKRPAHVILQEA
                     LEVAGRSDVIIAALGESSEMSGESSSRTNIEIPKLQQDLLRALVKTGKPVVLVLFTGR
                     PLAMKWESENVPAILNVWFAGSEAGYAITDALFGDVNPSGKLTTTWPQNVGQVPLFYN
                     HKNTGRPLGEGKWFEKFKTNYLDVSNDPVYPFGYGLSYTQFNYGELQLSKTNLKGNET
                     LKASVTITNTGQFAGKEVVQLYIRDVVGSVTRPVKELRGFQKIELQPGESRLVTFAIT
                     PEDLKFYNYDLKYDWEPGEFQIMIGTNSKDVKTGRVNWVK"
     gene            1918..>2230
                     /locus_tag="PK977_19340"
     CDS             1918..>2230
                     /locus_tag="PK977_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRF20346.1"
                     /translation="MNRKISFADNPVVAHRGAFKKNNLPENSIAALKQAIELHCTGSE
                     FDVRMTADDSLIINHDPHYHKLEIEKTSFAELSAFKLSNGEKLPTLREYLLTGLANNQ
                     QT"
BASE COUNT          626 a          370 c          511 g          555 t
ORIGIN      
        1 ctttctctcc gatggtggat atctgccgtg atccccgctg gggacgaatt gcagagggca
       61 gtggtgaaga tgcttacctc gggtcagaaa tagcaaaggc gatggtaaaa ggataccagg
      121 gaaatgatct gacaaaaaat aatacgatca tgtcatgtgt aaagcattac gcattgtatg
      181 gagccgctga ggcaggaagg gattacaata ccacagacat gagccggcaa agaatgtata
      241 atgattattt tccgccttat aaagcagcgg ttgatgccgg tgtgggaact gtgatggctt
      301 cttttaatga aatagatggt gttccggcta cggctaataa atggctgatg acagatgttc
      361 ttcgcaagca gtggaagttt aatggatttg ttgtaacgga ttatacgggt attaatgaaa
      421 tgatcgatca tggaatcggt gatttgcaaa cagtttctgc aagggccctt gatgcaggta
      481 tagatatgga tatggtgggc gaaggctttt taaccacctt gcaaaaatca ctaaaagaaa
      541 aaaagatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagag
      721 ctttgttttg cttcgaaatc aaaacaacgt tttgccgtta aagaaatcag gaactgttgc
      781 gttgattggc cccctgtcag ataacaaaga aaatatgcca ggcacatgga gtgtggctgc
      841 tgatttttca aaagccactt cgttgcttgc cggtatgaag gaactggtgg gggataaagt
      901 aaagatcctg catgccagag gctctaattt ggatgaagac agcctgtttg aagaaagggc
      961 cggtatgttt ggtaaatcat tacgcaggga taagcggcca gcccatgtaa ttcttcagga
     1021 agcactggaa gtcgccggtc ggtcagatgt gattattgct gcactgggtg aatcttctga
     1081 gatgagtgga gaaagcagca gccgtactaa tattgagata cctaaactgc aacaggatct
     1141 tttgagagca ttggttaaaa caggtaaacc agttgtattg gttttattta caggcaggcc
     1201 attggcaatg aagtgggaaa gtgaaaatgt acctgctata ctgaatgtgt ggtttgccgg
     1261 aagcgaagct ggctatgcta ttacagatgc attatttggt gatgtgaatc catcgggtaa
     1321 actgacaacc acctggccac aaaatgtggg tcaggttccc ttgttttata atcataagaa
     1381 tacaggccgt ccgctgggag aaggtaagtg gtttgaaaaa ttcaagacga attacctgga
     1441 tgtgagcaat gaccctgttt atccttttgg ttatggcctt agttacacac agttcaacta
     1501 tggtgaactg cagctcagca aaacaaattt gaaaggaaac gaaacattaa aggccagtgt
     1561 taccattacc aacaccggcc agtttgccgg taaggaagta gtgcagttgt atatccgtga
     1621 tgtagtaggc agtgtaacaa gaccagtaaa agaactcagg ggtttccaga agatcgaatt
     1681 acaacccgga gaatccagat tggtcacctt tgctattact cctgaggacc tgaagttcta
     1741 taattatgac ctgaagtatg attgggagcc gggcgaattt caaataatga tcggcaccaa
     1801 ttcaaaggat gtgaaaactg gtagggtaaa ctgggtaaaa taaataacgg aatgagactt
     1861 tgtttgctgt cttttcttgt cctctttata gcagggtgtt atacttccgg taaaaacatg
     1921 aacagaaaaa tatcttttgc tgataacccg gtggtagctc accggggtgc attcaaaaag
     1981 aataatcttc cggagaactc aattgctgca ttaaagcagg ccattgaact acactgtacc
     2041 ggatctgaat ttgatgttcg tatgactgca gatgattctc tcatcattaa tcatgatccg
     2101 cattaccata agctcgaaat tgaaaaaacc tcttttgctg aattatcagc attcaaactt
     2161 tctaatggcg aaaagttgcc taccctccgg gaatatttat tgacggggtt agccaataat
     2221 cagcagacaa
//
LOCUS       DAORLS010000424         2223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_26316_length_2223_cov_1.552122, whole genome shotgun sequence.
ACCESSION   DAORLS010000424 DAORLS010000000
VERSION     DAORLS010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26316_length_2223_cov_1.552122"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>426)
                     /locus_tag="PK977_19345"
     CDS             complement(<1..>426)
                     /locus_tag="PK977_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20347.1"
                     /translation="FDAMAKEKAAFMLWLGDAWYTRDVDYYSEWGLWYRASRDRSLPV
                     LQPFLKAMPQFGIWDDHDYGPNDIGKNYILKDATRKVFNSYFCNPSAGENGQGIYTMT
                     SWGDVDVFMTDDRWWRSADRMKDSVNGEPNPEKRMLGKQQ"
     assembly_gap    427..499
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<500..738)
                     /locus_tag="PK977_19350"
     CDS             complement(<500..738)
                     /locus_tag="PK977_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20348.1"
                     /translation="MQYAKKGEAKSKTVHYKGELGNEFNPITFTLGGLDFNTTYQYKL
                     FVNGKTTSAGGEFTTKDLWQWRKPVPDFSFLTGSCS"
     assembly_gap    835..851
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1063..1091
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1092..1333)
                     /locus_tag="PK977_19355"
     CDS             complement(<1092..1333)
                     /locus_tag="PK977_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20349.1"
                     /translation="MITYFRVGSIGPYQLPYTSDAGGATNTINCIYNLQKIIIYRHTL
                     GSCRFTSGDPGSTPVLINLPQSLEYRYILIPGAVSGG"
     assembly_gap    1446..1470
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1471..1716)
                     /locus_tag="PK977_19360"
     CDS             complement(<1471..1716)
                     /locus_tag="PK977_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20350.1"
                     /translation="MRKFQTLSLLALAISFLAVSCTKEGPEGPAGATGAQGPTGATGA
                     TGGTGPAGPTGPAGPTGPTGPQGPTGPQGPQGPAGSAN"
     gene            1895..>2223
                     /locus_tag="PK977_19365"
     CDS             1895..>2223
                     /locus_tag="PK977_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20351.1"
                     /translation="MRICLLSALFLLGSFSAIAQFSKGDKMIGASVGSVVVNSGSSDI
                     SVAQIGSNTSRITNFNININPSFGWFISDKTVVGGAVIINPYNNKTTYEQSGSTYQSD
                     KNSNFSIG"
BASE COUNT          579 a          465 c          435 g          600 t
ORIGIN      
        1 ttgctgtttg cccaacattc ttttttccgg gttgggttct ccgtttactg aatccttcat
       61 gcggtcggcg ctgcgccacc agcgatcatc tgtcataaaa acatccacat ctccccaact
      121 ggtcattgtg taaatgccct gcccgttttc gccggcggaa gggttgcaga agtagctgtt
      181 gaaaacctta cgggtggcat ccttcaggat ataatttttg ccaatatcat tcggaccata
      241 gtcatgatca tcccaaatgc caaactgcgg catggctttt aagaatggct gaagcaccgg
      301 caatgatcgg tctctgctgg ctctgtacca caaaccccac tcactgtaat aatccacatc
      361 ccgggtgtac caggcatcgc ccagccaaag cataaaagct gctttttctt tggccattgc
      421 atcaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnng aacaggaacc tgtcagaaaa ctaaagtccg gcaccggttt
      541 acgccattgc cagagatctt tggtggtaaa ttcaccccct gcgctggtcg tttttccatt
      601 cacaaacagt ttatactggt aagtagtatt aaaatctaac cctccaaggg taaaagtaat
      661 ggggttgaat tcatttccca gttcaccttt ataatgtact gttttcgatt tggcttcccc
      721 tttttttgca tattgcaagg aaactaattt tacttccgga gatacctcca cccaaatctt
      781 gacatcccta agttcaacgg gtcccagcat agggccggat aggatccgtg gctgnnnnnn
      841 nnnnnnnnnn natttgcatg gataatacag taaaaaatat tactttgata attactttca
      901 taaccattta ttaaagcgca aaatacaaaa gccgttcctt attggaacgg ctcctgaaaa
      961 agataaattg taagatttat ggttgtattt taagcaaagc acacaattct tgatatggca
     1021 tatttttaag ttgtgtggct gtataaccag cagcagggcc tgnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nccaccacta accgcaccag gaattaaaat ataacgatac tctaaagact
     1141 gtggtaagtt aattaacact ggggtcgaac ccggatcacc agaagtgaat ctgcagcttc
     1201 ccaatgtatg acggtaaata attatttttt gaagattgta tatacaatta attgtattgg
     1261 ttgcacctcc tgcatcgctg gtatatggca actggtaggg accgatagaa cctacccgga
     1321 aataggtaat catcaccccc tggttaagaa ttgcagttga aagtgatgga gcatcaatgt
     1381 gacgaactct taagcaagtg ccatcaatag tggtatccct ttggctgtaa ggacttgtaa
     1441 cccatnnnnn nnnnnnnnnn nnnnnnnnnn attggctgat ccggcaggac cttgcgggcc
     1501 ttgtggacca gtgggacctt gagggcctgt tgggcctgtt ggaccagcag gacctgtagg
     1561 gccagcagga cctgtaccac ctgtagcacc agtagcgccg gtgggacctt gtgcacctgt
     1621 agcgccggca gggccttcag gaccttcctt tgtacaacta acggctaaaa aagatatagc
     1681 taatgctaaa agggatagag tttgaaactt tctcatagcg ttttgatttg cgttttttgg
     1741 ttttggtttt aaaagtaaag ggaattttcc gcttcaccaa gacaatttaa aggtaaagca
     1801 aaaaaaaaca ttccgccatc aattttcaaa aaaaatccta tcctcaatgc atatacaatc
     1861 cccctatatt tgccgggtaa tcaaaactgt atatatgcgt atttgcctgc tctctgccct
     1921 ttttttgtta ggttcttttt ctgccattgc ccagttttca aaaggagata aaatgatagg
     1981 tgcatctgtc ggcagtgtgg tagtcaattc gggcagttcg gacatttctg tcgcccaaat
     2041 tggcagcaat acttcccgga ttaccaactt caacattaat atcaatcctt cattcggttg
     2101 gtttatatct gacaaaacgg ttgtgggtgg tgctgttatt ataaacccgt acaataacaa
     2161 aactacctac gaacaaagtg gttcaactta ccagagtgat aagaacagca attttagtat
     2221 agg
//
LOCUS       DAORLS010000425         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_26573_length_2206_cov_1.942352, whole genome shotgun sequence.
ACCESSION   DAORLS010000425 DAORLS010000000
VERSION     DAORLS010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26573_length_2206_cov_1.942352"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK977_19370"
     CDS             <1..503
                     /locus_tag="PK977_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRF20352.1"
                     /translation="HESGSDRIAEAAAEMEVDIIVNVQGDTPFVKKEPLEKLLKQFDD
                     PAVQVASMMQVLTDRNDIMDPNFVKVAVDKNMNSLFFSRAVIPYPRDKEFPTTYYEHI
                     GVYAFRKQALMNFTQWPMTPLESAEKVECLRYLENGIPLRMIVVDYMGVEIDTPEDLE
                     KAAKLL"
     gene            563..1636
                     /locus_tag="PK977_19375"
     CDS             563..1636
                     /locus_tag="PK977_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HRF20353.1"
                     /translation="MKFRNFKMVGYVVYGRGSFNQLDEILAPHRKDGKPMIFLVDHFF
                     EGKPLVSRVPLRGNDKIIFADVTYEPKTTYVDQLANQLKEEFGTVSGVIGIGGGSTMD
                     LAKAVSLMMNNPGSSADYQGWDLVKYPAVYKAGIPTLSGTGAEVSRTTVLTGPTKKLG
                     MNSDFTPFDQIVLDPELTKDAPVNQRFYTGMDCYIHCIESLEGTYLNEFSKSYGEKAL
                     QLCREVFVNKTAWDDDCDDKLMMASYAGGMSIAYSQVGVAHAVSYGLSYLLGTKHGIG
                     NCIVMNHLEEYYPAGVKEFKYMVEKNKIDIPTGICKGLSDDDFEKMINVSMGMKPLWE
                     NALGKDWEKIMTREKLRALYEKL"
     assembly_gap    1859..1985
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1986..>2206
                     /locus_tag="PK977_19380"
     CDS             <1986..>2206
                     /locus_tag="PK977_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HRF20354.1"
                     /translation="SLHWQQATQQVGQLNTIALLTYHINYYLRGLLQVLNGGALDIKD
                     KYSFAMPPVNNENDWQALSAELFSNAEKF"
BASE COUNT          589 a          455 c          515 g          520 t
ORIGIN      
        1 aacatgaaag cggcagcgac cgtattgctg aagcggcagc agagatggag gtagatatca
       61 ttgtgaatgt gcagggcgat accccgtttg taaaaaaaga gccgctggaa aaattattga
      121 agcagtttga tgatcccgct gtgcaggtag cctccatgat gcaggtgctg acggacagga
      181 atgatatcat ggatcccaac tttgtgaaag tggcagtaga caagaacatg aactctcttt
      241 tcttcagccg tgcagtaatc ccttacccga gagataaaga atttcccacc acatactatg
      301 agcatatcgg tgtatatgca ttccgcaagc aggcactcat gaattttacg caatggccca
      361 tgacaccgct ggagtcggct gaaaaggttg aatgtctccg ctatttagaa aacggcatcc
      421 cgctgcggat gatcgtagtg gattatatgg gcgtagaaat cgatacaccg gaagatttgg
      481 aaaaagctgc taaactattg tagattttag attgcagatt tcagattttt cggttcacct
      541 ttgcggtagt taaatcaatt acatgaagtt tcgcaatttc aaaatggtcg gctatgtagt
      601 gtatggccgg ggatcattca accagttaga tgaaatactg gcgccgcacc gcaaggatgg
      661 caagccgatg atatttttag tagatcattt ctttgaaggc aaaccgctcg ttagccgtgt
      721 gccgctgcgg ggcaatgaca agatcatctt tgccgatgtc acttacgaac ccaaaaccac
      781 ttatgtagat cagctggcca accaactgaa agaagagttt ggtaccgtca gcggtgttat
      841 cggtatcggc ggcggatcaa cgatggatct ggcaaaagcc gtttcgctga tgatgaacaa
      901 tcccggctcc tcggctgatt accagggctg ggacctggtg aaatatcctg ctgtgtacaa
      961 agccggtatt cctacactca gcggtacagg tgcagaagta tccagaacaa ctgtgctaac
     1021 cggcccgaca aaaaagctgg gcatgaattc tgacttcacg ccgttcgacc agatcgtact
     1081 cgaccctgaa ctcaccaaag atgcaccggt aaaccaacgc ttctacaccg gcatggattg
     1141 ttatatccat tgtattgaaa gtttggaagg cacttacctc aatgaattca gcaaaagcta
     1201 tggcgaaaaa gcgttgcagc tttgccggga agtgtttgtc aataaaacag catgggatga
     1261 tgattgtgat gataaactga tgatggcgtc ttatgccggt ggcatgagca ttgcctacag
     1321 ccaggtgggt gtggcgcatg cagtcagtta tggattaagt tatttgctcg gcaccaaaca
     1381 cggtatcggc aattgcatag taatgaacca tctcgaagaa tattatcccg caggagtaaa
     1441 agaattcaaa tacatggtgg agaaaaacaa gatcgatatc cccacaggta tctgcaaggg
     1501 actgagcgat gatgattttg aaaaaatgat caatgtatcc atgggcatga agccgctttg
     1561 ggaaaatgcg ctgggcaagg attgggaaaa gatcatgaca agagagaagc tgcgggccct
     1621 gtacgaaaag ctgtaacact gctctccaat ttttttgttt gtcacccttt gtcttgctga
     1681 gccctttctg ctttgtcatc ctgagcctgt cgaaggatgt gctacgaaga acgaacgcta
     1741 tatacaccga tgaaccacaa gctgagatta attaaacctt tcgtacatca ctcttcgaca
     1801 agctcagagt gacaacaggc tcaggatgac atttctcttg aatcggcctg cttatattnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnatccc ttcactggca gcaggccacg cagcaggtag ggcagctcaa taccattgcc
     2041 ctgctcactt atcatattaa ttattacctc cggggtttgc tgcaggtgct caatggtggt
     2101 gcgctggata tcaaagacaa atacagtttt gcgatgccgc cggtaaacaa tgaaaacgac
     2161 tggcaggccc ttagcgctga gttgttcagc aatgccgaaa aatttg
//
LOCUS       DAORLS010000426         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_26718_length_2197_cov_1.571429, whole genome shotgun sequence.
ACCESSION   DAORLS010000426 DAORLS010000000
VERSION     DAORLS010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26718_length_2197_cov_1.571429"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK977_19385"
     CDS             complement(<1..580)
                     /locus_tag="PK977_19385"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRF20355.1"
                     /translation="MKKILLLLVFGFGLMAMGIAQDEPEAEKKEAPKRKYARATFNAT
                     KIINMQSTEIVSKGNLQFLISHHFSNLWNKDAESGENFAQLLGLNSGVANTYLSFDYS
                     ITDWANIGWAAAGRSRFEGFAKFRLLRQQTGLKNVPVSVTWYSMAHVNAAKNADITFG
                     WNKWAYMHQLLIARKFSDKFSLQFNPALIHFNV"
     gene            complement(622..>983)
                     /locus_tag="PK977_19390"
     CDS             complement(622..>983)
                     /locus_tag="PK977_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20356.1"
                     /translation="NGGTHPGAGAIDFSPTEKNIIKRWIAQGEPYDDGAGCTVTGNLR
                     YTTDIVPIYNTSCKGATCHGGIAAPIDYAKFVAKKDVLLTILNSGGNTGHPGGALSLT
                     TCTVNKMKEWIAQGQPQ"
     assembly_gap    984..1011
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1012..1264)
                     /locus_tag="PK977_19395"
     CDS             complement(<1012..1264)
                     /locus_tag="PK977_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20357.1"
                     /translation="MKKKITLAALATSLMVYLLSSCYQNKEDILELPRVSFRNEVVPI
                     VTAGPCGCHNTSVNVRAVLFSDQRTNTIFYDAILARRGAL"
     gene            complement(1295..>2197)
                     /locus_tag="PK977_19400"
     CDS             complement(1295..>2197)
                     /locus_tag="PK977_19400"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20358.1"
                     /translation="RFEIYSQIMQYVKPGDPEGSKLWEYITTNDFDKAMPPVNSNHEL
                     GQRDKSIIYNWIKNGAKERPDLNDFRPAAIRVLVDGCSSANCHNTATATGGWARKGLI
                     PGLTSADTTQFTYINPTTGSVTVYCQLSNQTLMNQVWTAYKDSVKRFYADTVANASFR
                     PWKTVSTPVSALSTRGPLNNYDDILMDILYPKSVRTVSSVVYTDPVTLKGYYVRGNPL
                     VSTDCFVRRMDSTLIYRNPLTLVEASVSGNMAYDDGGFKPSEVALFKAWYFADPNIPD
                     VWKYGIGNVGIFKYRKTGNYIIKR"
BASE COUNT          602 a          469 c          452 g          646 t
ORIGIN      
        1 ccacattgaa atgaataagg gcaggattaa actgcaatga aaacttatct gagaacttac
       61 gggcaataag caactggtgc atataagccc atttgttcca tccaaaagtg atgtcggcat
      121 tcttggcagc atttacatgt gccatcgaat accaggtaac tgataccgga acattcttta
      181 atcctgtttg ctgacgtaac aggcgaaact tggcaaagcc ttcaaaacgg ctgcggcctg
      241 ctgcggccca tccaatattg gcccagtcgg taatggagta gtcaaatgaa agataagtgt
      301 tggctacacc ggagttcaga cccagcagtt gggcaaaatt ttcgccgctt tctgcatcct
      361 tattccacag gttgctgaag tggtgagaga tcaggaactg caggtttccc ttacttacga
      421 tttcagtact ctgcatattt atgatcttcg tagcattaaa ggttgccctg gcatattttc
      481 ttttaggagc ttcttttttt tcagcttcgg gttcgtcttg tgcaataccc attgccatca
      541 aaccgaatcc aaaaacgaga agcagtaata tctttttcat acgatcttat tttatagtaa
      601 gaacattttg tttattagct gttattgagg ttgcccctgg gcaatccatt ctttcatttt
      661 attgactgtg caggtagtaa gactcagcgc tccgccgggg tgaccggtat taccgccact
      721 attcaatatg gtcagcagta catctttttt ggccacaaat tttgcataat caataggagc
      781 tgcaatacca ccatggcagg ttgcaccttt acaactggta ttgtaaatag gaacgatatc
      841 ggtggtatat cttaaattac cagtaacggt acagccagca ccatcatcat aaggttcgcc
      901 ttgtgcaatc caccttttaa tgatattctt ctcagtagga gagaagtcaa tagctcctgc
      961 acccgggtgt gtgcccccgt taannnnnnn nnnnnnnnnn nnnnnnnnnn nacaatgcac
     1021 ctctgcgggc caggatggca tcatagaaaa tggtattagt tctttgatca gagaataata
     1081 cggcacgaac gttaacactg gtattgtggc agccacaagg gccagctgtt acaataggca
     1141 ctacttcatt ccggaaagaa actctcggta gctccagaat gtcttcttta ttttgataac
     1201 aggatgacaa caggtacacc atcagtgagg ttgccagcgc tgctaaggtt attttctttt
     1261 tcatatacct gagatttaaa atgagttgat ggggttatcg cttaatgata taatttccgg
     1321 tcttcctgta tttgaatatg ccgacattgc caataccata tttccataca tcaggaatat
     1381 tgggatcagc aaaataccag gccttgaaaa gggctacttc ggaaggttta aagccaccat
     1441 catcataagc catattacca ctaactgaag cttctacaag tgtcagcggg ttccgataga
     1501 ttagtgtaga atccatccgg cgaacaaaac agtctgtaga aaccagcggg ttacccctta
     1561 catagtatcc ttttaatgta accggatctg tatacactac ggaactaact gttcttactg
     1621 atttgggata aagaatatcc atcaggatgt catcatagtt attcaatggt cctcttgtac
     1681 ttaatgcaga aacaggagta ctgactgtct tccatggacg gaagctggca ttggccacag
     1741 tatccgcata aaatcttttc acactgtctt tataagcagt ccatacctgg ttcatcaggg
     1801 tttggttgga aagctggcaa taaacggtaa cggatcctgt tgttggattt atgtaagtga
     1861 actgtgtagt atccgcagag gtaagtcccg gaatcaatcc ttttcttgcc caaccacctg
     1921 tagcagtagc tgtattatga cagttggcag aactacaacc atcgactaat accctgatag
     1981 cagcaggtct gaagtcattc aggtcgggtc tttctttagc gccattttta atccagttat
     2041 aaataatgct cttgtctctt tgtcccaatt catgattgga atttacagga ggcattgctt
     2101 tatcaaaatc attggtagta atatattccc acaacttgct tccttccgga tcgccgggtt
     2161 ttacatattg cataatctgg gaatagatct caaagcg
//
LOCUS       DAORLS010000427         2171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_27118_length_2171_cov_1.440926, whole genome shotgun sequence.
ACCESSION   DAORLS010000427 DAORLS010000000
VERSION     DAORLS010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27118_length_2171_cov_1.440926"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PK977_19405"
     CDS             <1..1064
                     /locus_tag="PK977_19405"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF20359.1"
                     /translation="EEPLSVSYKGIEVYKLQQWTQGPAMLQALNILENYDLKSMGYNS
                     SKYIHTIYQAMNLAFADRDFYYGDPYFTPHEPVRGLLSKEYAKQRAALIKPDKNDPAI
                     GPGAPYPFEGKVNPYADLIKKWNISGEGNRNFLPAHDSTGTSFTDEVYMERLWMGTTS
                     VEAADKDGWVVSITPSGGWLPACIAGNTGVGMSQRLQSFVLDSSLNPFNVIEPGKRPR
                     VTLTPSLALKDGKPFLSFAVQGGDTQDQNLLQFFLNMVEFNMTVQQACEAANINTNQL
                     WLSLGGSKMTDRKPRAGEILVSNSTPDNVRSELQKMGYKVSTGNRTSGPINAIFFDWK
                     HGSFWGGSSNNGEDYGIGW"
     gene            1165..>1830
                     /locus_tag="PK977_19410"
     CDS             1165..>1830
                     /locus_tag="PK977_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20360.1"
                     /translation="MKLRPVFCYVTLPGIWVTNNKTRHVMRNTGLYISFFILLLPYSS
                     LQAQDYRAIHGSSYAGALGAANNPTSIVHVPFKWDVTPLAVQLKQSTNLLTINNFSLL
                     SPGGNVTVTANAGNQRRYLMSNQDIKVLNARIRLNEKSAIAFGASVRSYVSIKTSNLN
                     WQDTVANVRDFMRINMSNSPLSVDIRTNGWAEVYGSYARTVLENDNGILNAGITVKVN
                     RGLA"
     assembly_gap    1831..1952
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1953..>2171
                     /locus_tag="PK977_19415"
     CDS             <1953..>2171
                     /locus_tag="PK977_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20361.1"
                     /translation="AWDSAGSFSDQRKLFLKKTFSTIAFSIGAEYIIPAESENFYDYD
                     LKIGASLLDIGFNNFQYSNNSRRALMNRS"
BASE COUNT          596 a          400 c          460 g          593 t
ORIGIN      
        1 aagaggaacc cctttctgtt agttacaaag gcattgaagt atacaaatta cagcaatgga
       61 cacaaggtcc tgcgatgttg caagccttga acattctcga gaattatgac ctgaagtcta
      121 tgggttataa cagttcaaaa tatattcata ccatttacca ggctatgaat cttgcttttg
      181 ccgacaggga tttctattat ggcgatcctt actttacacc acatgagcct gttcgaggat
      241 tactaagcaa agaatatgca aagcaaagag ctgctttaat aaagcctgat aaaaatgatc
      301 cggcaatcgg gccaggtgct ccatatccat ttgaaggaaa ggtaaaccct tatgcggacc
      361 tgattaaaaa atggaatatc tccggagaag gtaatcgtaa ctttttaccg gcgcatgatt
      421 caacaggtac ttcttttaca gacgaagtat acatggaacg attgtggatg ggtaccacca
      481 gtgtggaagc tgctgataaa gacgggtggg tagtttctat tacaccaagt ggcggatggt
      541 tacctgcctg cattgccggt aataccggtg tgggcatgag ccagcgatta caaagctttg
      601 tactcgattc atctttaaat cctttcaacg taattgaacc gggaaaaagg ccaagggtga
      661 cattgacacc ttcgctggcg ttaaaagacg gcaaaccctt tctgtcattt gctgttcagg
      721 gtggcgatac ccaggatcaa aacctgttac agtttttcct gaatatggtg gagtttaaca
      781 tgaccgttca gcaggcctgc gaagcagcca atattaacac caaccagtta tggctttctt
      841 taggcggttc aaagatgact gaccggaagc caagagcagg agaaatattg gtgagtaatt
      901 ccacaccgga taatgtaaga agcgaattac agaagatggg ttataaagtg agtactggta
      961 acagaaccag cggacctatc aatgccattt tctttgactg gaaacacgga agtttttggg
     1021 gtggtagcag taacaatggt gaagattatg gtataggctg gtaattattt actgattttt
     1081 caatcggacc tactgattat tgtcatctgc cttcatttac atcttcggga actttatgaa
     1141 agtttttacc cgaaagaata ctttatgaag ctgaggcccg ttttttgtta tgttacatta
     1201 cccggcattt gggttactaa taacaaaacc aggcatgtga tgaggaatac cggtttatac
     1261 atttcatttt ttatactctt acttccatat agctcactgc aggcgcagga ttacagggcc
     1321 attcacggat catcctatgc aggtgccctt ggagccgcta ataacccgac ttctattgta
     1381 catgttcctt ttaaatggga tgtaactcca ctggctgttc agcttaaaca gagtaccaac
     1441 ttacttacca tcaataattt ttcactcctg tctcccggcg gtaatgttac cgttactgcc
     1501 aatgcaggaa atcaaaggcg gtacctgatg agtaaccagg atataaaagt tttgaatgcg
     1561 aggataaggc tgaatgaaaa gtcagcaatt gcttttggtg cttctgtcag gtcttatgtt
     1621 tctattaaaa cttcaaatct taactggcag gataccgttg ccaatgtcag ggattttatg
     1681 cggattaata tgtccaattc gcccttgtct gtcgatataa gaacaaatgg ctgggcagag
     1741 gtatatggca gttatgcaag aaccgttctt gaaaatgata atggcatcct taatgcaggc
     1801 attactgtaa aggtgaacag ggggcttgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcatggga tagtgcaggt agtttcagtg
     1981 accagcgtaa attgtttttg aagaaaacat tctcaaccat tgctttcagc attggagcag
     2041 aatatatcat tccggcagaa tcagagaatt tctatgatta tgatcttaag atcggcgctt
     2101 ctttgctgga tattgggttc aataactttc agtacagtaa taacagccgc cgggctctaa
     2161 tgaaccgttc a
//
LOCUS       DAORLS010000428         2166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_27198_length_2166_cov_2.226433, whole genome shotgun sequence.
ACCESSION   DAORLS010000428 DAORLS010000000
VERSION     DAORLS010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27198_length_2166_cov_2.226433"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PK977_19420"
     CDS             complement(<1..518)
                     /locus_tag="PK977_19420"
                     /inference="COORDINATES: protein motif:HMM:NF025123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HRF20362.1"
                     /translation="MSRFAVLKQIMLQHRNRLVLTYILFSLEMLGNLLRPFFLGLAIN
                     DLIKGSYQGLILLSAIHLGWLLIGTIRHRLDTRTYSAIYTSLVTKFLARRYNKSDVSK
                     LSAHSNLAREFVDFLEFDLVYIIEALYSIFGSLILLFFYDTSVVLVCLSILLPVVAIS
                     YLYGKRMKMLNK"
     gene            complement(582..2030)
                     /locus_tag="PK977_19425"
     CDS             complement(582..2030)
                     /locus_tag="PK977_19425"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRF20363.1"
                     /translation="MKVCVLQPDYSTTGVDYKNYDPPRDLSLLIPEASVHNIFLNKLT
                     TYKQLKELSKQGYDIFVNLCEGYLEWEVPSIDVIYTLELLNLPFTGPSTNLYDPPKEL
                     MKYLAYCEGVKTPGYVIIHELKDVDEALKFLKFPLFVKPAKAGDSLGVDEHSLVHNKI
                     ELQQKCISIIEEYGPLLVEEYIAGREFTVLVAANTDEHSCTVFKPVEYVFPEGRAFKT
                     YALKTSELHPDCNVPCNDVLLGNELRSATEKIFKGFNGVGYARLDFRVNDHNEVYFLE
                     INFTCSVFYKDGYEGSADYILKHDGIGQAGFLRHIINEGIARHRRKMKPYIMKGNSIA
                     GYGIYASRSIRKGEVIFKGEGKSQRIITKRYVEKNWNDDEKLHFRRYAYPISDEVFIL
                     WDEDPAEWAPQNHCCDPNTAFDGLNVIALKDIPQGTELTLDYADFLDENMEPFDCQCG
                     SSLCRGKISGKHGNTLNLREQSLFGLNDLPTQ"
BASE COUNT          640 a          455 c          431 g          640 t
ORIGIN      
        1 tgcttgttaa gcattttcat tcttttacca tacaggtagc tgatggctac cactggcagt
       61 agaatactca ggcaaaccag tactactgag gtgtcataga aaaacaacag aataagggaa
      121 ccgaagatgc tgtaaagcgc ttcaatgata taaacaaggt caaactccaa aaagtcaaca
      181 aactctcttg caaggttaga gtgtgcactt aattttgaaa catctgattt gttatatctt
      241 cttgcaagga attttgttac caatgatgtg taaatagcgg aataggttct tgtatcaagc
      301 cggtgacgga tagtgcctat caaaagccag cccaaatgaa tggcagacaa caaaattaaa
      361 ccctggtaac ttccctttat caggtcattt atggctaaac ccaggaaaaa gggcctgagc
      421 aggttaccca gcatttccag tgaaaaaaga atgtaggtca gcaccaggcg gttgcggtgt
      481 tgtaacatga tctgtttaag tacggcaaaa cgggacatag ttcatctatt taagataaat
      541 catcttatga gtcagtaaaa tttaatggag taagcctaag actattgggt tggtaagtcg
      601 tttaacccga aaagggattg ttcccgcaaa tttaaagtat tgccgtgttt gcccgaaatc
      661 tttccccggc ataatgaaga accgcactga caatcaaatg gttccatgtt ttcatccaaa
      721 aaatcagcat aatcaagagt gagttcagtg ccttgcggaa tatcttttaa tgcaataaca
      781 ttaagcccgt caaaagccgt gttggggtca cagcaatgat tttgtggcgc ccattctgcc
      841 ggatcttcat cccagagaat gaacacttca tcgctgatag gatatgcata acggcggaaa
      901 tgcagttttt catcatcgtt ccagttcttt tcaacatatc gtttggtaat gatgcgctgg
      961 gactttcctt cgcctttaaa aatcacttca ccttttcgga ttgaacgaga ggcatagata
     1021 ccatatccgg caatagaatt acccttcata atatagggct tcatctttcg ccggtgtcgg
     1081 gctataccct cgttaataat atgcctgaga aatccggcct ggccgatacc atcatgtttt
     1141 aaaatataat cagcagaacc ttcgtagcca tccttataaa aaacggaaca ggtaaaattt
     1201 atttcaagaa agtaaacctc attatgatca ttcacccgga aatcaagtct tgcataacca
     1261 acaccgttaa aacctttaaa gatcttttcg gtagcagagc gaagttcgtt cccgagtaac
     1321 acatcgttgc acggtacatt gcaatcggga tgaagctctg atgtctttag cgcatatgtt
     1381 ttaaaagctc ttccttctgg gaaaacatat tcgacgggtt tgaaaaccgt acagctatgt
     1441 tcgtcagtat tggcagctac caaaacagtg aactctctgc cggcaatata ttcttccacc
     1501 agtaaagggc cgtattcctc aattatgctg atacattttt gctgcagttc aattttgtta
     1561 tgcaccagcg aatgttcatc tactcccagg ctatctccgg ccttggccgg tttaacaaat
     1621 aaaggaaact ttaaaaattt taaagcctca tccacatcct ttagttcatg aatgattaca
     1681 tagcccggtg tttttacccc ttcgcaatag gcaaggtatt tcatcagttc tttgggaggg
     1741 tcatagaggt tggtgctcgg tcctgtaaac ggaaggttca gtaattccag tgtatagatt
     1801 acatcaatac tcggcacttc ccattctaaa taaccctcgc agagattgac gaaaatatca
     1861 taaccctgtt tgctgagttc tttcagttgt ttgtacgtag tgagtttatt cagaaaaata
     1921 ttgtgtaccg atgcttcggg aattaacagg gaaagatccc ttggcggatc ataattctta
     1981 taatcaaccc cggtagtgga atagtcgggc tgcagtacac aaactttcat gatttgctaa
     2041 ggtaatgagg cgggaacact atttcacctt aataatagtt ttgttttttg ctgtccgttt
     2101 attccttaca ggtgcttgag tgagcacact tcttcttagc gcatctttta atatctcccc
     2161 aatcac
//
LOCUS       DAORLS010000429         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_27271_length_2161_cov_1.697531, whole genome shotgun sequence.
ACCESSION   DAORLS010000429 DAORLS010000000
VERSION     DAORLS010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27271_length_2161_cov_1.697531"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    217..372
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<373..826)
                     /locus_tag="PK977_19430"
     CDS             complement(<373..826)
                     /locus_tag="PK977_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20364.1"
                     /translation="MKPAEQLSASYRDPSGFMFRVNGIYYRQVNKLFKEDFDYFISSG
                     CYDELVRTGLLIPHETINENLTGSADWYQTLKPEQLQFVSYPYEWSFDMLKDAALLTL
                     KILKKSLKKEMILKDATPFNIQFHRGKMIFIDTLSFEKYHEEEPWAAYR"
     gene            complement(990..2117)
                     /locus_tag="PK977_19435"
     CDS             complement(990..2117)
                     /locus_tag="PK977_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF20365.1"
                     /translation="MAKAVKKAKSGSAKKSIITDKAFEFFKNYINNASPVGFETWGQK
                     LWIEYLKPYVDSHYVDPYGTAVGIINPDHPFKVVIEAHADEISWFVNYVTPEGLIYLK
                     RNGGVDHQTAPASRVIIHGKKGPVKAVFGWPAIHTRIGNPDQKEPTPRTDNLWLDCGA
                     RNRKQVEELGIHIGAVVTYQDGFDELANGNYIGRAFDNRIGGFMIAEVARLLRENNKK
                     LPFGLYIVNAVQEEIGLRGAEMIARRIKPNIAIVTDVTHDTTTPMINKNIEGDVSTGK
                     GPSLSYAPAVHNKLLALVEDIAKNKKIPVQWRALSRSTGTDTDSFAYSNDGCPSVLIS
                     IPLRYMHTTVEMLHRDDIENTIKLMYETVLTLSPKTNLNYL"
BASE COUNT          562 a          396 c          389 g          658 t
ORIGIN      
        1 gccaggtaat tgtttcttcc cgccgcttct tcataataag aagaccatgt gcttgtctgc
       61 gacgaaagct tcagtttatt gaccagaatt tccaaactgt caacgaggtt tattagcctt
      121 tttcggttaa atagaacctt ttttttggtt gccgatttgg ggctattact aaatcttgca
      181 ttcaggtgaa catgtaaata agtatgaaat gaaaatnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nngcctgtat gctgcccatg gttcttcttc gtgatatttt tcgaaagaaa
      421 gtgtgtcgat aaatatcatt tttcctctgt gaaactgaat attaaaggga gtagcatcct
      481 tcaatatcat ttccttttta agggactttt ttaaaatttt caatgtaagt aatgctgcat
      541 cttttagcat gtcaaaactc cattcgtagg gataggatac aaattggagt tgctctggtt
      601 ttagggtttg gtaccagtcg gctgaacctg tcaggttctc attaattgtt tcatggggga
      661 taagcaatcc tgttcttact aattcatcgt agcatcctga agaaataaaa taatcaaaat
      721 cttctttgaa taatttattt acttgtctgt aataaatacc gttaaccctg aacataaatc
      781 cagaaggatc tctgtaggaa gcagacagtt gttcagcagg tttcatcagg aagaagaatt
      841 tttatccgtt ttacggtttg aacgggtaaa aaaagctttg atgtagttct ttatgttacg
      901 aaaaaaaaag gcaattccta atacaccggc aataattagt tgcaccaaat aacttccgct
      961 tcccggatca atatatagac aatataatat cacaggtagt taaggtttgt tttaggagac
     1021 aatgtcagca cagtttcata catcagcttg attgtatttt caatatcatc acggtgcaac
     1081 atctccactg ttgtatgcat atagcgcagc gggatagaaa ttaaaacaga agggcagcca
     1141 tcatttgagt atgcgaatga atcagtatcc gtaccggtgc tacgacttaa agccctccat
     1201 tgtaccggta tttttttatt ttttgcaata tcttccacca atgcaaggag tttgttatgg
     1261 acagctggag cataagatag agacgggcct ttacctgtac ttacatcacc ttcaatattt
     1321 ttgttaatca tcggggtggt agtatcatgt gttacatctg taacgatggc aatattgggt
     1381 tttatgcgcc tggcgatcat ttccgctcca cgaagaccta tttcttcctg tactgcatta
     1441 acaatatata acccgaaagg aagcttttta ttattttctc taagaagtct tgctacttcg
     1501 gcaatcataa atccaccaat gcggttatca aaggcacggc caatataatt gccgttggcc
     1561 agttcgtcaa aaccgtcctg gtaagttacc actgccccaa tgtggatacc gagttcttcc
     1621 acttgcttac ggtttcttgc accgcaatca agccacaaat tatctgttct tggagtgggt
     1681 tctttctgat cagggttacc aatacgggta tgtatggcag gccagccaaa aaccgctttt
     1741 accgggcctt ttttaccgtg gataattacc ctggatgccg gtgctgtttg atgatcgaca
     1801 ccgccgtttc tttttaggta aatcagtcct tccggggtta cataattaac aaaccagctt
     1861 atctcgtcgg catgggcctc tattacaact ttaaatggat gatccggatt tatgatacca
     1921 acagcagttc catatggatc aacgtagtgg ctgtcaacat acggtttcag gtattcaatc
     1981 cacaactttt gcccccaggt ttcgaagcca actggcgagg cattattgat ataattttta
     2041 aaaaattcaa atgccttatc agttataatt gattttttag cagaacccga ttttgccttt
     2101 tttactgctt ttgccatgtc cgttgattta agttgagcta attgcaacag cgaagataag
     2161 a
//
LOCUS       DAORLS010000430         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_27397_length_2153_cov_1.919924, whole genome shotgun sequence.
ACCESSION   DAORLS010000430 DAORLS010000000
VERSION     DAORLS010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27397_length_2153_cov_1.919924"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PK977_19440"
     CDS             complement(<1..611)
                     /locus_tag="PK977_19440"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20366.1"
                     /translation="MKLHSTYMGMKLDSPIIVSACTLSEQTDNIVKMEDNGAGAVVMF
                     SLFEEQIRMEEARFKGVMSETTYAFPEALDYFPDLDDFNVGVDDYLENIRKAKERVNI
                     PIIGSLNGITTEGWIDYSRLMEQAGADAIEVNIFFIPGDIAMSSSEVEHRYLNIIETI
                     KQTVKIPVAVKLNPYFSATGNMALRMKNSGADALVLFNRFYQP"
     assembly_gap    771..780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<781..>1922)
                     /locus_tag="PK977_19445"
     CDS             complement(<781..>1922)
                     /locus_tag="PK977_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF20367.1"
                     /translation="NIPWNDRSKIDFSTVHGAQFLEPLFEFSGACAGCGETPYLKLLS
                     QLFGDRLLVANATGCSSIYGGNLPTTPWAKNKDGRGPAWSNSLFEDNAEFGLGMRITA
                     DKQLDVAASLLNQLKGELGEAYVKEILEAKQTLESEIVEQRNRVDGLKEKLKGMNSPA
                     ASHLLSVADQLVRRSVWLVGGDGWAYDIGYGGLDHALASGRNINVLVLDTEVYSNTGG
                     QSSKSTPTAATAKFAAGGKTVGKKDLAMQAISYGNVYVARVAFGANPQQALLAMREAE
                     AYDGPSLILAYSHCIAHGYDLKNGLDQQNKAVASGHWPLLRYNPVLRKKNQNPFVLDS
                     PRPTVSLKDYAYNELRYKVLTITNPEAADELMKHAQDLVNLKWKTY"
     assembly_gap    1923..1934
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1935..>2153)
                     /locus_tag="PK977_19450"
     CDS             complement(<1935..>2153)
                     /locus_tag="PK977_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF20368.1"
                     /translation="DDFKSAPINARGFPETQYSLQVYLEDCTGCNLCVEVCPATDPSN
                     RSKKAINMGEKEAILQKEQKNIEFFENIP"
BASE COUNT          567 a          532 c          404 g          628 t
ORIGIN      
        1 tccggctggt aaaaacggtt aaacaagacc aatgcatcgg ccccggaatt cttcattcgc
       61 aaggccatat tgccagtagc actgaaataa ggattcaact tcacggcaac aggaatcttt
      121 accgtctgct tgatcgtttc aataatattc agatagcgat gctctacttc agaagaggac
      181 atagcaatat cacccggaat aaagaaaata ttcacttcta tagcatctgc cccagcctgt
      241 tccatcaacc gggaataatc gatccagccc tccgtagtaa tgccattcaa acttccgata
      301 ataggaatat tcactctttc ttttgccttc ctgatatttt ccaggtaatc atccacccct
      361 acattaaagt catcgaggtc gggaaaataa tccaatgcct cgggaaaagc ataagttgtc
      421 tccgacatca ctcctttgaa ccgggcttct tccatcctga tctgctcttc aaacaaggaa
      481 aacatcacta ccgcaccggc cccgttgtct tccatcttca caatattatc tgtttgttca
      541 gataaagtgc aggcagaaac aataatcggg gaatccagtt tcatccccat gtatgtggaa
      601 tgtagtttca tattatttgt tacgttttta atgtttggtg ttcatggatt atagttcacg
      661 gcttttactt atcaaacaac ggatgagcaa aacaaccata tgctatcaat catgaactgc
      721 atttaagcta ccggcacaaa atcgcttgcc tttttggtag cgagttcttc nnnnnnnnnn
      781 atatgttttc catttaaggt ttaccaggtc ctgcgcatgc ttcatcagtt catccgccgc
      841 ttccgggtta gttatagtca gtactttata ccgtagttca ttgtaggcat agtccttcag
      901 cgatactgtt ggccttggtg aatccagtac aaacggattt tgatttttct ttctgagcac
      961 cgggttatag cgcagcaatg gccagtgtcc gcttgcaaca gctttatttt gctgatccag
     1021 tccgttcttc aggtcatagc catgcgcaat acagtgacta taggccagta taagtgaagg
     1081 accgtcataa gcttcagctt ctctcatggc cagcaaggcc tgctgtggat tagcgccaaa
     1141 ggcaactctt gccacataca cgttaccata agagatagcc tgcatagcaa ggtctttctt
     1201 acccaccgtt ttacctccgg ctgcaaattt tgctgtggct gccgtaggag tagatttaga
     1261 ggactgacct cctgtattag aatatacttc cgtatctaaa accaatacgt tgatatttct
     1321 tccacttgcc aatgcatgat ccaggccgcc atagccaata tcataagccc atccgtcacc
     1381 accaaccagc catacactgc ggcgaaccag ctgatcagct actgacagta aatgactggc
     1441 agccggcgaa ttcatcccct ttaatttttc tttaagccca tctacccggt tgcgttgttc
     1501 tacaatttca gactccagtg tttgctttgc ttctaaaatt tcttttacat atgcttcacc
     1561 caattcgcct ttcaattgat ttaaaagact tgcagctaca tccaactgtt tgtcggccgt
     1621 gattcgcata cctaacccga actctgcatt gtcttcaaac aaggagttgc tccaggccgg
     1681 gccacggcca tctttgtttt tagcccatgg cgtggtggga agattaccgc cataaataga
     1741 agaacaaccc gtagcgttcg ccaccagtaa cctgtcgcca aataattgcg acaacaactt
     1801 caaataaggt gtttcaccac aacctgcaca ggctccgctg aattcaaaca atggctccag
     1861 gaactgggct ccatgtactg tcgaaaaatc aatttttgac cggtcgttcc agggaatgtt
     1921 tannnnnnnn nnnngggaat gttttcaaaa aattcaatat tcttttgttc tttttgcaat
     1981 atggcctctt tttctcccat attgattgcc tttttggaac gattggaagg atctgtcgcc
     2041 ggacaaactt ctacacaaag attacagccg gtacaatctt ccaggtaaac ctgcaatgaa
     2101 tattgtgttt ccggaaaacc tcttgcattg atcggtgcag atttgaaatc atc
//
LOCUS       DAORLS010000431         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_27406_length_2152_cov_2.065808, whole genome shotgun sequence.
ACCESSION   DAORLS010000431 DAORLS010000000
VERSION     DAORLS010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27406_length_2152_cov_2.065808"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK977_19455"
     CDS             <1..202
                     /locus_tag="PK977_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20369.1"
                     /translation="LMPDIMFEYDENDRVIQKITTTSSQSLGYLIWRYIFDNKGLKTK
                     EALFNGDKELTGKIEYSYSFFQ"
     gene            287..>565
                     /locus_tag="PK977_19460"
     CDS             287..>565
                     /locus_tag="PK977_19460"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRF20370.1"
                     /translation="MLGKLSDKQIDELLFSQSIGRLGCYADGKIYVVPVTYVYDGQNI
                     ICHSREGMKLEMMRKNPDVCFEVDKMENMANWQSVIAWGTFVELNGEEA"
     assembly_gap    566..602
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            767..1237
                     /locus_tag="PK977_19465"
     CDS             767..1237
                     /locus_tag="PK977_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRF20371.1"
                     /translation="MTKQKKSWTEKLNDSKDLPKVIKLTPAQQKKWGKGTMAIPSPLE
                     VNGIMKRVAPGKLITINRIREKIARKHKADIGCPITTGIFSWIAANAAEEAEKKGLKK
                     ITPYWRTLKEGGIINEKYPGGIEKQALLLKQEGHLIVQKGKKWMVEGWEKRLMK"
     gene            1258..2088
                     /locus_tag="PK977_19470"
     CDS             1258..2088
                     /locus_tag="PK977_19470"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRF20372.1"
                     /translation="MDYPAFIVNHWQDEQKVFRKNLEILKDHPDRNAVHDIRVAIKKL
                     RAILELYIPLTGEKLWENPLKETETLFKVLGRQRDIDICLEILKPLVIESGFTFHELS
                     SFFESMLKKARQWTSVAVRQQDENEIGRIKVLLKSEESSLTNEDAVRKTMTIITGQLL
                     QSRQLLKFPHQLRQHLKKILYWVRMLPGELPVIFHEKELHEITDDLGLWQDNEIFLAR
                     SRHFRKDYLPKTYDEYGQLRILETESKKQNRLLLNKALRKTRKLLKTVALNEKEKALS
                     "
BASE COUNT          779 a          382 c          459 g          495 t
ORIGIN      
        1 gctgatgcca gatatcatgt ttgagtatga tgaaaatgac agggtcattc aaaaaatcac
       61 cactacatca agccaaagcc ttggctacct gatctggaga tatatttttg acaacaaagg
      121 gcttaaaaca aaagaagctt tatttaacgg ggataaagaa ctgaccggta aaatagaata
      181 cagctactct ttctttcaat gagttgaatt aaatcataat cccccctgac tcctctcaat
      241 ggaaatattg gataattgca gttatttgct tttgtaaact gcagctatgc ttggtaaatt
      301 atccgacaaa caaatagatg aattgttatt tagccaatcg attggccgtt tgggttgtta
      361 tgccgatgga aaaatctatg tagtgcctgt cacatatgta tatgatgggc aaaatattat
      421 ttgtcatagc cgggaaggca tgaaactgga aatgatgcga aaaaatcctg atgtatgctt
      481 tgaagtagat aaaatggaga atatggcaaa ctggcaaagt gtaattgcct ggggcacatt
      541 tgtagaatta aatggcgaag aagctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnatgttaat gaaattacag ccccaactga ccagtgagac ggcccggccc cacgatcctg
      661 gcaatagcga agaaagaagg gcaacaaggg ggttaacagc tataatttac cagatagtca
      721 ttaaagaaag aaccggacgc tttgaaaagc atttgaaata aacaaaatga cgaaacaaaa
      781 aaaaagctgg accgaaaaac taaacgacag taaagacctc ccaaaggtga taaagctgac
      841 acccgcccag caaaaaaaat ggggaaaagg cacgatggca attccttcac cactggaagt
      901 caatggaatc atgaaaagag ttgcacccgg aaaacttatt actatcaacc ggataaggga
      961 aaaaattgcc aggaaacata aagctgatat cgggtgcccg atcactactg ggatattctc
     1021 ctggattgct gccaatgcag ccgaagaagc tgaaaagaaa ggattaaaaa agataactcc
     1081 ctactggcga actttaaaag aaggtggtat aatcaatgaa aaatacccgg gtggaataga
     1141 aaagcaggct ttactgctaa aacaggaggg acatcttatt gtacaaaagg gaaaaaagtg
     1201 gatggtagag ggctgggaaa aaagactaat gaaataaaac catgaagctt caataaaatg
     1261 gattaccctg cattcatagt aaaccactgg caggatgaac aaaaggtttt caggaaaaac
     1321 cttgagatac taaaagatca tccagacagg aatgcggtac atgatataag ggtagccata
     1381 aagaaactaa gggcaatact ggaactttat ataccgctga caggtgagaa actatgggaa
     1441 aatccgctca aagagacgga aacgcttttt aaagtgttag gccgccagcg ggacattgat
     1501 atctgtttgg aaatactaaa acctcttgtt attgaatcag gatttacttt ccatgagctt
     1561 tcctcctttt ttgagtcaat gttaaagaag gccagacaat ggacttctgt tgcagtcagg
     1621 caacaggatg aaaatgaaat cggccgaatt aaggttctgt tgaaaagtga agaaagtagt
     1681 ttgacaaatg aagatgccgt acgaaaaaca atgacaataa ttacgggcca gttattgcaa
     1741 agcaggcaat tattaaaatt tcctcaccaa ttacgacagc acttaaaaaa aatattgtat
     1801 tgggtgcgga tgcttcccgg cgaactcccg gtaattttcc acgagaaaga gttacatgaa
     1861 ataacagatg atcttggttt atggcaggat aatgagatat tcctggcccg gtcccgccac
     1921 ttcagaaaag attatttacc gaagacatat gatgaatacg ggcaattaag aattcttgag
     1981 acagaatcaa aaaaacaaaa caggttactg ctaaataaag ctctccgaaa gacaagaaaa
     2041 ttactgaaaa ctgtagcttt aaatgaaaaa gaaaaggccc tcagctaagc tgaagacctt
     2101 tgtgcccgag actggattcg aaccagcaca ccctttcggg cgccaccacc tc
//
LOCUS       DAORLS010000432         2149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_27469_length_2149_cov_1.667144, whole genome shotgun sequence.
ACCESSION   DAORLS010000432 DAORLS010000000
VERSION     DAORLS010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27469_length_2149_cov_1.667144"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>301)
                     /locus_tag="PK977_19475"
     CDS             complement(<1..>301)
                     /locus_tag="PK977_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20373.1"
                     /translation="GLDINDVASIDLLKDAATTAIYGARAANGVVLVTTKKGQKGKTR
                     VTYSFSHTTNYIRRNPSEYLSAADYIRMNRLGIQARFRGDSLDNNTGAMNNDRNQ"
     assembly_gap    302..416
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<417..864)
                     /locus_tag="PK977_19480"
     CDS             complement(<417..864)
                     /locus_tag="PK977_19480"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF20374.1"
                     /translation="MKSKCLRLCLLALFSLVVSLTFAQPKRVTGKVLSDDSRPLSGVS
                     VVIKGKPGGTQTNTNGEYSIEASVGDILVFSYSGFSTREMKVGSSPVIDLSLETKVSE
                     LDAVVVTGYGTQKRKEVTGAVTTVNPKVFEHSPSSNVATVLQGNTPG"
     gene            1015..1728
                     /locus_tag="PK977_19485"
     CDS             1015..1728
                     /locus_tag="PK977_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRF20375.1"
                     /translation="MMNTTSLTSSLRSIDTSSLVDKVESQLVELLQQRKLKVGDSIPK
                     EIELAESIGVSRTVIREALLRLRLMGLIESKKKKGAVITSPDIFAVMGKSLNPHILDE
                     GTLKEIFEIRLVLEIGMADLLFHRVTKEDIAELKEIVSNEPPATQFHLFNIEHEIAFH
                     GKLYEITGNETMKKFQRMLLPIFDYVHNSGLLKKQSKLKTFVSHKELVKILEKGTPEK
                     FRNGMRNHLENHFARLFNK"
BASE COUNT          656 a          399 c          366 g          613 t
ORIGIN      
        1 aactggtttc tgtcattatt catggcacct gtgttattat ccaatgaatc acccctgaaa
       61 cgggcctgga tgcccagccg gttcattctt atataatctg ctgcacttag atactcagat
      121 gggttacgac gaatatagtt tgtagtatga ctaaaggaat aggtaaccct tgttttccct
      181 ttctgtcctt ttttggttgt taccaatacc acgccattag cagctcttgc accataaatg
      241 gcggtggtgg ctgcatcttt cagcaagtca atacttgcca catcgtttat gtccaggcca
      301 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcc
      421 tggagtattt ccctgaagta cggttgcaac atttgagctc gggctgtgct caaatacctt
      481 tggattaaca gtggtaacag cacccgttac ttcttttctt ttttgtgtac cataaccggt
      541 tacaaccact gcatccagtt cactcacttt tgtttccaat gacaaatcaa taaccggact
      601 actgcctact ttcatttcac gggtagaaaa ccctgaataa gaaaatacga gtatatctcc
      661 tacagaagct tcaatgctgt actctccatt ggtattggtt tgagtaccgc ccggcttacc
      721 cttgataaca actgatacac cggatagggg ccttgaatca tcgcttagta ctttcccggt
      781 aactcgtttt ggttgggcaa atgtgagaga tacaaccagg gaaaataatg caaggagaca
      841 aagccttaaa cattttgatt tcatgacaag cagttttgtt tatttgtatt atgtatgaca
      901 taataaaatt atgtagtttt atatttcaaa taccaaaacc tagaaaaata agcgaaacga
      961 ttgcgtaagc gatttgccga agcttttcaa aaaataacta atattgaatg atttatgatg
     1021 aacacaacct cgcttacctc atcactgcgg tcaatagata ccagttcatt ggttgataaa
     1081 gttgaatctc aactagttga actattacag caacgcaagc taaaagtagg agacagcata
     1141 cccaaggaga tcgaactggc tgaatcaatc ggggtaagcc gtacagtaat ccgggaagcc
     1201 ctattacggc tgcggcttat gggtttaatt gagtctaaaa agaaaaaagg tgctgttatt
     1261 accagccctg acatctttgc tgtaatgggt aaaagtctga acccgcatat actggacgaa
     1321 ggcaccttga aagaaatatt tgaaatccgc cttgttttag aaattggtat ggccgacctt
     1381 ttatttcatc gtgttacaaa agaagacatt gcagagctaa aagaaattgt cagtaatgag
     1441 cctccggcta cacaatttca cttattcaat atagaacatg aaattgcctt tcacggaaaa
     1501 ctttatgaaa taaccggtaa tgaaactatg aaaaaatttc agcgtatgct gctgcctatt
     1561 tttgactatg tacataacag cggattgcta aaaaagcaat caaagctaaa aacatttgtt
     1621 tcccacaaag aattagtaaa aattcttgaa aaaggcacac cagaaaagtt cagaaacggt
     1681 atgagaaacc atcttgaaaa tcattttgca cggctattca ataaatgaga attttttgac
     1741 ctgtgttgat catcaggtat cgtcacctta tcttggcgga tacctgataa ggcgacctct
     1801 tttcgtttgc tgctatttaa tagattatta ttctgaataa cgccaggttc taagctaata
     1861 cgaatacgct tttgtcttgg agataaaatg tatccaggaa acccttaatg caaatactat
     1921 aagcaacacc tactataaaa atttttatga aatggttatc aaatatgagc catcattact
     1981 gtatatcccc agatcaaatg aaccagtttc aagttaaact ttaactcaat gtggtacaat
     2041 ctaatcatca aaatactacc gtaatgaata ttccattatt atttaaaatg ttgcgaaata
     2101 aaataattat tgttcattaa aaccaacgga aatgaatgta ataatccag
//
LOCUS       DAORLS010000433         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_28013_length_2115_cov_1.799029, whole genome shotgun sequence.
ACCESSION   DAORLS010000433 DAORLS010000000
VERSION     DAORLS010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28013_length_2115_cov_1.799029"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK977_19490"
     CDS             <1..308
                     /locus_tag="PK977_19490"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20376.1"
                     /translation="VNECADRHLLLDAFQAVFVYPNPTNGIVNMRLNTDLYSSLGLRV
                     FNAMGQEVKSQVFTGLAFGSVVSTDLSRLPAGTYQLYMYNTQNGFIKKAVSVIYYKR"
     gene            510..>1803
                     /locus_tag="PK977_19495"
     CDS             510..>1803
                     /locus_tag="PK977_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HRF20377.1"
                     /translation="MSKLYKALLAICIVSILPFTATAQQGKYWSATDAASVTTKHKAV
                     ARQSFPKDFLVFNLDIPELKQQLFSVVDRPGSSTVISLPNAEGTLELFEIFEASNFAP
                     ALQAQFPDIRAFSGRGITNPGSMLKLSYANNWVQTMIFRADGKPNEYIEPYSQDGRSV
                     AVFKSQRAKGGLPWTCSTQEQEMAQGLNNRVYSSRIESNAGQLKTMRLAQSCNGEYAN
                     FFGATSSAQVALVLAAFNATLTRTNGCYEKDLAIHLNLVPETVNVIYYDPATDPYTTL
                     GQWNTQLQIALNTTLTGPGTPLAANNAAYDIGHMFGASGGGGNAGCIGCVCVDGVASG
                     TGSTKGRGITSPADGIPQGDNFDIDYVAHEVGHQLGANHTFSMSNEGFGVNKEVGSGI
                     TIMGYAGITSQDVAPHSIDIFHQASIQQVQANMVGKTCP"
     assembly_gap    1804..1820
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1821..>2115
                     /locus_tag="PK977_19500"
     CDS             <1821..>2115
                     /locus_tag="PK977_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20378.1"
                     /translation="QAGINATPVVAALTNYTIPISTPFALTGSATDADAGDVLTYCWE
                     QDDDGAGQTGNNSVARENKPTGPNWLTWPATTSPTRLMPRLQTILTGANLTGPL"
BASE COUNT          592 a          455 c          476 g          575 t
ORIGIN      
        1 aggtgaatga gtgtgcagac cgccacttgt tactggatgc cttccaggct gtgtttgttt
       61 acccgaatcc gaccaatggt atagttaaca tgcggttgaa tactgacttg tatagcagcc
      121 ttggtttaag ggtatttaat gcaatgggac aggaagttaa gagccaggtg tttactggtc
      181 ttgcttttgg aagtgtcgta tcaaccgacc tcagcaggtt accagcaggc acctatcagt
      241 tgtatatgta caacacacag aacggcttta tcaagaaagc agtgagtgtg atttactata
      301 aaagataatc agtgttataa aaatcaacaa ttaaggcctg gtcattccga ccgggccttt
      361 ttttgaggct aatcaagact ttttctgttt ttatacaaac cagggttgaa aaaaaacggt
      421 tggggcatgt caattaattc ttaattttag cggccttttg gcggaatccc aatctgtccg
      481 gagcactttt aaaaaccaaa tattttttta tgagtaaatt gtacaaagct ttactggcta
      541 tttgtatagt tagtatttta ccttttactg ctacagccca gcaaggcaaa tactggtcag
      601 ctacagatgc tgccagtgta actacaaagc acaaagctgt tgcaagacaa tccttcccca
      661 aagacttcct ggtattcaat ctggatattc cggaattaaa acaacaactg ttctctgttg
      721 ttgaccgacc aggaagttca actgtgattt ctcttccaaa tgcggaagga actctggagc
      781 ttttcgaaat ttttgaggct tcaaattttg cccctgcatt acaggcgcag tttccggata
      841 tccgggcttt ttctggaaga ggtatcacca accccggttc gatgctgaag ttgagttatg
      901 caaacaactg ggtgcaaaca atgattttca gagcggatgg taaacccaac gagtatatcg
      961 agccttattc tcaggacggc cgttccgtag ccgttttcaa atcacaaaga gcaaaaggag
     1021 gtttgccatg gacatgttct acacaggagc aggaaatggc acaaggttta aacaacaggg
     1081 tttattcatc aaggatcgaa agcaacgccg gtcagttaaa aacaatgcga cttgcacagt
     1141 catgcaatgg tgaatatgct aactttttcg gtgctaccag ttcggctcaa gtagcattgg
     1201 tactggctgc attcaatgct acgttaactc gtaccaacgg atgttatgaa aaagatcttg
     1261 ccattcacct gaatcttgtt ccagaaaccg tgaatgtgat ctattatgat cctgccactg
     1321 atccgtatac tacacttgga caatggaata ctcaattaca aattgcactc aatacaactc
     1381 tcacaggtcc aggaacacca cttgctgcta ataacgctgc ttatgatatt ggacatatgt
     1441 ttggcgcttc aggtggaggt ggaaatgccg gttgtatcgg ttgtgtttgt gttgatggcg
     1501 ttgccagcgg tacaggatca accaaaggaa gaggtatcac atcccctgca gatggtattc
     1561 cccagggaga taactttgat atcgattatg tagctcatga agtgggacat cagctgggcg
     1621 ccaatcatac attctccatg tcaaatgaag gtttcggagt aaataaagaa gtgggttccg
     1681 gtataactat tatgggctat gccggtatca catcacagga tgtggcaccg cattcaattg
     1741 atatctttca ccaggcatca atacagcagg ttcaggcaaa tatggtcggt aaaacttgcc
     1801 cggnnnnnnn nnnnnnnnnn ccaggccggt attaatgcta ctccggttgt tgctgcatta
     1861 accaactaca caattcctat cagtactccc tttgcattaa ccgggtcagc tacagatgct
     1921 gatgccggag atgtacttac ctattgctgg gagcaggacg atgatggtgc aggtcaaaca
     1981 ggaaataaca gtgtggcccg tgagaacaaa ccaacaggtc caaactggct gacctggccc
     2041 gcaacgacat cgcctaccag gctgatgccc agactgcaaa ccattcttac cggtgcaaac
     2101 ctgacgggac ctctt
//
LOCUS       DAORLS010000434         2114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_28025_length_2114_cov_2.444390, whole genome shotgun sequence.
ACCESSION   DAORLS010000434 DAORLS010000000
VERSION     DAORLS010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28025_length_2114_cov_2.444390"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1138)
                     /locus_tag="PK977_19505"
     CDS             complement(26..1138)
                     /locus_tag="PK977_19505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF20379.1"
                     /translation="MSGIDTFIERKMKELRIVGMGASIIIDKKVIWSQGYGYADRENK
                     TPFTTSTIMNIASISKTFTGVCMMKAFEEGKLSLDEDINAYLPFKVVNPNFPGEKITL
                     RQLATHTSGLADRYPFYSDSTYFYGGKKPESLGEFLRNYFVPGGRYYSADNFLKSKPG
                     TYRDYSNIGAGLAGYIIELRTRKKLNEYGKEHIFKPLEMSYSGWSLAEINTNDHSKLY
                     KKEGDSVKSIQLYEGTTYPDGGVRTSVSELSKFFICLLNDGKLNKTRILQKKTIGEML
                     RFQFNETNKPENEKLEKLNYGIFWATKMGATRIGHNGSDPGVRTFMLSDLAKEIAIVI
                     FFNTSLSDAEEGEYFDIYNGIYNYALDLKKDKTTSR"
     gene            1335..>1569
                     /locus_tag="PK977_19510"
     CDS             1335..>1569
                     /locus_tag="PK977_19510"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRF20380.1"
                     /translation="MQELDEFDKKILNLLQQNNRITAEKLGSMLSLSTSAVQRRLKRL
                     RQDKIIEADISIISQSAVGFGITCIVDVTLHLGN"
     assembly_gap    1570..1587
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1588..1809
                     /locus_tag="PK977_19515"
     CDS             <1588..1809
                     /locus_tag="PK977_19515"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRF20381.1"
                     /translation="VIDSYKELMSACAEVMQCYYVTGTYDFVLIVNTKDMKHYESFSK
                     KYFMDNPKVKQFYTHVVMDKVKSGFSVNI"
BASE COUNT          627 a          403 c          390 g          676 t
ORIGIN      
        1 gcgcccggct tgaggaaata cgatgttagc ggctggttgt tttatctttt tttaagtcta
       61 aagcgtaatt gtatattcca ttataaatgt caaagtattc accttcttca gcgtcactga
      121 gtgaagtgtt aaaaaaaata actatagcaa tttctttggc cagatcagaa agcataaagg
      181 ttcttacacc cgggtcagac ccattatgac caatccgggt agcacccatt tttgttgccc
      241 agaaaatgcc gtaatttaac ttttctaatt tttcattttc aggtttattg gtttcattga
      301 attgaaaccg aagcatttcg ccaatagtct tcttttgcag gatgcgggtt ttatttaatt
      361 ttccgtcatt caacaggcaa ataaaaaact tcgacaactc acttacagat gtacggacac
      421 cgccatcggg ataagttgtt ccttcatata attgaattga tttaacactg tctccctctt
      481 ttttgtagag tttagagtga tcattcgtat tgatctctgc caatgaccaa ccggagtatg
      541 acatttccag gggtttgaaa atatgctcct tgccatattc gtttagtttt tttcttgtcc
      601 tcaactcaat aatataacct gccaaaccag ccccaatatt agaataatca cgataagtgc
      661 ctggttttga ttttaaaaaa ttatctgctg aataatacct gcccccgggc acaaaatagt
      721 ttcttagaaa ttcgcctaga ctctcaggct ttttccctcc ataaaaataa gtactgtcag
      781 aataaaaagg gtaacggtct gctagcccgg aagtatgtgt tgccagctgt cttaaggtaa
      841 ttttttcacc cgggaaattg gggttaacca ctttgaaggg caaataagca ttgatatcct
      901 catctagtga cagctttcct tcttcaaatg ctttcatcat acaaacacct gtaaaggtct
      961 tactgatcga agcaatattc atgatcgttg aagttgtgaa gggtgtttta ttctctctgt
     1021 ccgcatagcc atacccctgt gaccagatta cttttttatc tatgatgatc gatgctccca
     1081 tgccaacaat tctcaattct ttcattttcc tttcgataaa tgtgtcaata ccactcattg
     1141 tttggcatct tagttcttga aagggggaga tggctaataa aaaaaagggg attgctaaca
     1201 ttggtctcat tgttattact ttcttgttta taaaatatgt tgtgcaatat tatgctcagc
     1261 aggttgactt ttttaactaa attgccactt tgaacgcaca aaaaagttaa taatatttat
     1321 taaacgggta aatcatgcag gaattggacg aatttgacaa gaagatctta aatctacttc
     1381 agcagaataa caggattacc gctgaaaaac tcggaagtat gttaagcctg agtaccagtg
     1441 ccgttcaaag aaggcttaaa aggctgcggc aggataaaat catcgaagct gatatatcaa
     1501 ttatctcgca atcagcagtc ggttttggca ttacatgcat agtcgacgta acattacacc
     1561 ttgggaattn nnnnnnnnnn nnnnnnngtg attgatagtt ataaggaatt gatgtctgcc
     1621 tgcgctgaag taatgcaatg ttattacgtt accggaactt atgattttgt attgatcgtg
     1681 aataccaaag acatgaaaca ttatgaaagt ttttccaaaa aatattttat ggacaacccc
     1741 aaagtaaaac agttttacac tcatgtggtg atggacaaag taaaatctgg atttagtgta
     1801 aatatttaga attgtaaatc ttcattttat gctcaatttt aattaatgta gtttgggaac
     1861 tgtctgtatc cgaagtctac tatacaattg ccgctaacgg ccactgcttg acgccagttg
     1921 gggaattagg accactgtcg cctgtaacgg tcagaccgaa aataccgatt ttttgaataa
     1981 gaacgtaccc aaggttgcgc cactaagagc gtagcaaaat ggcgcaacct ggttaggatt
     2041 tcctgtcgca cattaaccta tgccctaaga acctaagtag ctgaatttca acgtgtcggc
     2101 cccaattttt ttaa
//
LOCUS       DAORLS010000435         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_28050_length_2112_cov_1.571706, whole genome shotgun sequence.
ACCESSION   DAORLS010000435 DAORLS010000000
VERSION     DAORLS010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28050_length_2112_cov_1.571706"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>215
                     /locus_tag="PK977_19520"
     CDS             <1..>215
                     /locus_tag="PK977_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20382.1"
                     /translation="AVANGLSIKEQPLPFKFGEDFGWYTTEYKSALFGIGAGIHHPAL
                     HHADYDFPDAIIETGIKMFGSIIEQVL"
     assembly_gap    216..225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            311..>576
                     /locus_tag="PK977_19525"
     CDS             311..>576
                     /locus_tag="PK977_19525"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRF20383.1"
                     /translation="MQKNRIAYIDQIKVFLTCLVVAHHAAQAYGPTGGVWVISDKAKA
                     SWLQDFFFINASYMMGLYFFISGYFMVFSIRRKTKYQFVKDRLK"
     assembly_gap    577..586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <587..1382
                     /locus_tag="PK977_19530"
     CDS             <587..1382
                     /locus_tag="PK977_19530"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRF20384.1"
                     /translation="IPLLFFTFLVFLPFNYFLSGSKENLFLFFADTYINKPPLATGHL
                     WFVASLLAYSFIYLILFGKHFSASADKAVPFRTYYILIYILLLTLATAVVRLKYPIDQ
                     WRTWGIPVEVAHLPQYLSLFLIGTVFNRLGWLTDIKLKTGIFFLMIAIVTYTSQYWLP
                     HSISGHWLAEPLAESLLCVGISLGIIVLFRHYSNKMNFASRLLSENAYGIYLFHLLIV
                     IMLQKMILSWNAAAELKFLTVTILAIIFSLLLSHFLRKIKTLRNII"
     gene            1401..1802
                     /locus_tag="PK977_19535"
     CDS             1401..1802
                     /locus_tag="PK977_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF20385.1"
                     /translation="MEAVQFRIARPTNRLSEIVEFYTKGLGMEIIGSFSGHAGYDGVM
                     LGMPDSKYHLEFTCHIDQPLLNEPSKENLLVFYYDDPEKYFAANNRLQAMNCLPVIPE
                     NPYWEDKSKTYEDPDKWRIVLFNGLFTAEKK"
     gene            complement(1809..>2112)
                     /locus_tag="PK977_19540"
     CDS             complement(1809..>2112)
                     /locus_tag="PK977_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRF20386.1"
                     /translation="VQVNNSHTDWRRTGSLWAIQDVKETYAKDNEWFALHITVKGKHI
                     TVAINDKTVVDYTEPDNVQREEGMKDRIISSGTFALQGHDPKSKVYYRNIRVKLLT"
BASE COUNT          594 a          449 c          412 g          637 t
ORIGIN      
        1 agctgttgcc aacggccttt ccataaaaga acagccgttg cctttcaaat tcggcgaaga
       61 ttttggatgg tacactactg aatataagtc tgccctgttt ggtatcggtg ccggtattca
      121 tcatccggcc ctgcaccatg cggattatga tttcccggat gcaatcattg aaactggaat
      181 aaaaatgttt gggagtatta ttgagcaggt gcttannnnn nnnnntcaca ttaaataatg
      241 tcagaaaaaa aacgaatacc gtagtttcgt tcatatgaag ctttcccgtt acaataatcg
      301 tttgtagtct atgcaaaaaa acaggatcgc ctatattgat cagatcaaag tatttctcac
      361 ctgtcttgta gtcgctcatc acgccgccca ggcatacgga cccaccggtg gtgtgtgggt
      421 aataagtgac aaagccaaag catcatggct gcaggatttt ttcttcatca atgcatcata
      481 catgatgggc ttgtactttt ttatttccgg gtactttatg gttttcagta tacgccgtaa
      541 aacaaaatac cagttcgtaa aagacaggtt gaaaagnnnn nnnnnnaatc cccttattgt
      601 tttttacgtt cctcgttttt cttcctttca attatttctt atccggcagc aaagagaatc
      661 ttttcctgtt ttttgcagac acttatatta ataagccgcc actggcaaca ggacatttat
      721 ggtttgtagc ctcattgctt gcttactcat ttatttactt aattctcttt ggaaaacatt
      781 tttctgcatc cgccgataaa gcagttccgt tccgcacata ctatattctt atttacatac
      841 tgctactgac actggcaact gctgtagtac ggttgaagta ccccattgat cagtggagaa
      901 cctggggcat cccggtagaa gtagcacacc tcccgcaata cctgagcctt ttcctgatcg
      961 gtaccgtttt caacaggttg ggctggttga ctgatataaa actaaaaacc gggatatttt
     1021 tcttaatgat tgccatagtt acttacacat ctcagtactg gctacctcat tccatcagcg
     1081 gtcattggct ggcagagcca ttggccgaat cattattatg tgtgggaata agcctgggga
     1141 taatagtgct gttcaggcac tatagcaata agatgaactt tgccagcagg ctgttgtccg
     1201 aaaatgctta tgggatttac ctttttcacc tgctcatagt catcatgctt caaaaaatga
     1261 tactcagctg gaatgctgcc gccgaattaa aatttttgac agtaaccata ttggcaataa
     1321 tattttcgct gttactttca catttcttga gaaaaataaa aacactaagg aatatcatat
     1381 aatctgaaaa aattagaaaa atggaagcag tacaattccg aattgcaaga ccaaccaaca
     1441 ggctctccga aatagttgag ttttacacta aggggctggg gatggaaata attggcagtt
     1501 tttccgggca tgctggttat gatggcgtaa tgctgggaat gcctgatagt aaatatcatc
     1561 ttgaattcac ctgccacatt gaccagcctt tattaaacga accaagcaag gaaaatctac
     1621 tcgtatttta ttatgatgat cccgaaaaat acttcgcagc caataatcgg ctgcaggcta
     1681 tgaattgcct tccagttata ccagaaaatc cttactggga agacaaaagc aaaacttatg
     1741 aagacccgga taaatggaga attgtattat ttaatggcct ttttacagct gaaaagaaat
     1801 gattagcatc aggtaagcaa ctttaccctg atattgcggt aatacacttt gctcttcgga
     1861 tcatgcccct gcaaagcaaa agtgccggaa gaaatgatgc gatccttcat tccttcttcc
     1921 cgctgcacat tatcgggttc ggtgtaatcc accaccgttt tatcattaat ggcgactgtg
     1981 atatgtttgc ctttcaccgt aatatgcagg gcaaaccact cgttatcctt tgcataagtt
     2041 tccttcacat cctgtatagc ccagaggcta ccggtgcgcc gccagtcggt atgtgaatta
     2101 ttcacctgta ct
//
LOCUS       DAORLS010000436         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_28144_length_2106_cov_1.901024, whole genome shotgun sequence.
ACCESSION   DAORLS010000436 DAORLS010000000
VERSION     DAORLS010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28144_length_2106_cov_1.901024"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>660)
                     /locus_tag="PK977_19545"
     CDS             complement(<1..>660)
                     /locus_tag="PK977_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20387.1"
                     /translation="GFQAKYGDKMSSVLDIQYKKPKRFGGSAYVGILEQGLQFEGTNK
                     NSKFSYLLGIRNRSNRNLLSRQDTKGNYVPSSADFQALLSYQFSPKWQAELLSNISQT
                     KFTLIPQASQLTTSVFSPVFSATIGVDINFEGREKDRYQTNMLGFALTNQVNKKLKLK
                     WMLSRFENDEEENIDIIGAYLFGERDFDKRNPTYGLIVNPLGAGIYQNWARNVLNVEN
                     WN"
     assembly_gap    661..684
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<685..1319)
                     /locus_tag="PK977_19550"
     CDS             complement(<685..1319)
                     /locus_tag="PK977_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20388.1"
                     /translation="MNRLRYFFLLTCCLISLFSFSQKKTAFVSGKVVDENENPLAKVS
                     IVILGQSKGIVTNDSGYFRIKVAADKAFALVFTYTGFKAEQKNFLLNEGEEETITIRM
                     ERGEKVLQEVIVTDQRDRREAGLIKPNPKTILNLPSAVTGVESLIKIFVGSNNELTSQ
                     YSVRGGSYDENLIYVNDFEVFRPYLIRSGQQEGLSFINPEMVRSISFYNGG"
     gene            complement(1376..>2106)
                     /locus_tag="PK977_19555"
     CDS             complement(1376..>2106)
                     /locus_tag="PK977_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20389.1"
                     /translation="TTAPPPPKVTGLAVKGSGSYVVAKTSDNLSSLSLDYITRNGNET
                     AFCFTYTNNSGNNSMMTIWMDNYKLYDRNTQQYYSASHINLPGVDAPKAVFDGESVRF
                     TVTFTNLPANLTNFDLRESDCTETAFCFSGLSINNYTKSSYTYSDIVKTYELKNQGKL
                     TIFSRYGSSGDINFWLADNYTGKLTRYITNSSYSPSCGDEGALTLTISEGSYRWRASD
                     EKYNWQDTKYVTANTCNSISLIVK"
BASE COUNT          555 a          432 c          438 g          657 t
ORIGIN      
        1 attccagttt tcaacattca gtacatttct tgcccagttc tggtaaatgc cggcacccag
       61 cgggtttaca attaagccat aagtaggatt tctcttgtca aaatctcttt cgccaaatag
      121 atatgcacca ataatatcaa tgttttcttc ctcgtcattt tcaaaacggg aaagcatcca
      181 ttttagtttt agttttttat ttacctggtt ggtcagtgca aaaccaagca tattggtctg
      241 atagcggtct ttttcccttc cttcaaaatt gatatcaaca ccaatagtgg cgctgaaaac
      301 aggggagaag acggatgtgg taagctggct ggcctgtggt atcagggtga actttgtttg
      361 tgaaatatta ctaagtagct cagcctgcca ttttggacta aactggtatg aaagcaacgc
      421 ctggaaatct gctgaagaag gaacataatt accttttgta tcctgacggc tcagcaggtt
      481 tcggttgctc cggttgcgaa tgcctagcag gtaactgaat ttactgttct tattagtgcc
      541 ttcgaattgc aggccttgtt ccaatattcc aacataagca gatccgccaa atcgctttgg
      601 tttcttgtat tggatgtcaa gcacactgct catcttatca ccatatttgg cctggaagcc
      661 nnnnnnnnnn nnnnnnnnnn nnnnaaacct ccgttataaa aactgatact cctcaccatt
      721 tccgggttga taaaactcaa tccttcctgt tggccgcttc ttattaagta gggacggaat
      781 acttcaaagt catttacata aatgaggttc tcatcataac tgccaccccg aactgagtac
      841 tgtgaggtta attcattgtt cgagccaaca aatattttga tcaggctctc tacccctgta
      901 acggctgaag gaagattaag aatagttttt gggttaggct taattaaacc agcctctctt
      961 cggtcccttt ggtcggttac aattacctcc tgcaatactt tttctcctct ttccatgcgg
     1021 atggtgatag tttcttcttc cccttcattc agcaggaaat tcttttgttc agctttgaaa
     1081 ccggtatagg taaaaactag ggcgaaagct ttatcggcag ctacttttat cctgaaataa
     1141 ccggaatcgt tggttacaat acctttcgac tgaccgagaa tgacaatgga aactttggct
     1201 aacggatttt cattttcatc caccacttta ccggatacaa aagcagtttt cttctgtgaa
     1261 aaagaaaaca gggagataag gcagcaggtt agtaaaaaaa agtagcgcag ccggttcatc
     1321 gcaataaaaa taattcattt ttgatgaagc gctgcgcttt tgttggtcac acttgctatt
     1381 ttacgattaa cgatatgctg ttgcaggtgt tggcagtcac atacttggtg tcctgccagt
     1441 tatacttttc atcggatgct ctccagcgat aacttccttc actgatagta agtgtaagtg
     1501 ctccttcatc tccgcaggat gggctgtaac tgctgttagt gatatacctt gttaattttc
     1561 ctgtatagtt atctgccagc cagaaattaa tatcgccgga ggaaccatag cgggaaaaaa
     1621 tggtgagttt tccctggttt ttcaactcat aggttttcac gatatcactg tatgtatacg
     1681 atgattttgt atagttgttg atgcttaagc cggagaagca aaatgccgtt tcagtacaat
     1741 cgctttccct caggtcgaaa ttagtaagat tggcaggcag gttggtgaat gtaacggtga
     1801 accgtacact ttctccatcg aatactgctt tgggtgcatc aaccccgggc aggttgatgt
     1861 gactggctga ataatattgt tgtgtattcc tgtcatagag cttatagtta tccatccaga
     1921 tcgtcatcat ggaattatta ccactgttat tggtataggt gaagcaaaag gcggtttcat
     1981 tgccattccg ggtaatataa tcaagcgaaa gagaggaaag attgtctgat gttttagcta
     2041 caacatatga gccgcttccc tttacagcaa gacccgtaac ctttggtggt ggcggagcag
     2101 tagtat
//
LOCUS       DAORLS010000437         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_28346_length_2094_cov_1.819519, whole genome shotgun sequence.
ACCESSION   DAORLS010000437 DAORLS010000000
VERSION     DAORLS010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28346_length_2094_cov_1.819519"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PK977_19560"
     CDS             <1..337
                     /locus_tag="PK977_19560"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRF20390.1"
                     /translation="WPVITYGIEYSGKEIGDAIQQKNWMEQPVYYWDPVLSPSGMAFY
                     NGTAIPEWKYNLFIGGLSSAHICRLVIVNKKAVGEERLLTDKYERLGMWRIITECCIL
                     LLMEETCTG"
     gene            431..877
                     /locus_tag="PK977_19565"
     CDS             431..877
                     /locus_tag="PK977_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20391.1"
                     /translation="MKVRSFIFVFSLLFAIHLHGQVTPGPRDKQPKSSDTTNIPKQTI
                     REHYDSLINDCNEMIKKLDKYIQIIQQDIESQQKQLKELADEVNKPVKAAQTMSKEEI
                     EKKTVVIKTQLESLNNALKIKLEQVEAQKNRIKELEKEKQELLRKF"
     gene            947..>2094
                     /locus_tag="PK977_19570"
     CDS             947..>2094
                     /locus_tag="PK977_19570"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF20392.1"
                     /translation="MIKNCTLVLLFLTVVFKNTSAQSAYTFTYNFNEPENNITYHAFL
                     IRNNDGSGLVRIRYSLPGNEEDILTEAFIDEFNPPDKNDDPDTSLLIIKAVNLSYIQG
                     NDKLKFAPPAFVFRYNSQNNLFEPAGVSNETNYAISSSVSFQWTLHEGAALNKDIVSI
                     FFSDDEDFYINLFRPVTRSLSPAEKNIKMHLLIVADTVDATIGKAVSIDITRVTELFS
                     SISNYLGIKLNTYILAGKSFGKSNTESALTKLRPAPNDIVIFYYSGHGFRIPEKPRAF
                     PNMKLKNVKTPRTAFRDSLVWAKASRTANISQSMNVADIFQTITKKGARMNLVISDCC
                     NDDIFSVNFKSTKPSGTKSSNIKWSEQNIRALFLDPTPVSILATACTET"
BASE COUNT          740 a          413 c          381 g          560 t
ORIGIN      
        1 gtggcctgtt atcacatatg gaattgaata cagcggtaaa gaaatcgggg atgccataca
       61 gcaaaaaaac tggatggaac aacctgttta ctactgggat cctgttctct ctcccagcgg
      121 aatggctttt tataatggca ccgccattcc cgaatggaaa tacaacctct ttattggcgg
      181 actaagcagt gctcatatct gcaggctggt gatcgttaat aaaaaagcgg tgggtgaaga
      241 aagattgcta accgataaat atgaacggtt agggatgtgg cgtattataa cggaatgttg
      301 tatactgtta ctgatggagg aaacttgtac agggtaagta aaaaacaata attagttaca
      361 agccattatc aaagaatccc tgttacagct caacacacat aaatagctaa taaaaagcta
      421 tcttcagtat atgaaggtaa ggtctttcat atttgtcttc tcgctgcttt tcgcaattca
      481 tctgcacggc caggtaacac ccggaccaag ggataagcaa cctaaatctt cggatactac
      541 taatataccc aagcaaacaa tcagggaaca ttatgattcc ctgatcaatg attgcaatga
      601 aatgataaaa aaactggata aatatattca gatcattcag caggacatag aaagccagca
      661 gaagcaattg aaagaacttg ccgatgaagt aaacaaacct gtaaaagcag cacaaacaat
      721 gtcgaaagag gaaatagaaa agaaaacagt agttatcaaa acccagctcg aaagccttaa
      781 caatgcactt aagattaaac tggaacaggt agaagcccaa aaaaatagaa taaaagaact
      841 ggaaaaagag aaacaggaat tattgcgtaa gttttgaaaa ggaagcaata aaagcattcc
      901 ccaaaaaaac tttttcaaat tcagaaacaa gccagagcaa aaatcaatga taaagaattg
      961 cacattagta ctactgtttc taactgttgt ttttaaaaat acatcggcac aatctgccta
     1021 cacttttaca tacaatttca atgaacctga aaacaatatt acttaccatg cctttttaat
     1081 taggaataat gatggcagtg gcctagttcg tataaggtac tcactcccgg gcaatgaaga
     1141 ggacatactt accgaagcct ttattgacga attcaacccg cccgataaaa atgatgaccc
     1201 ggacacaagc ttgcttatca ttaaagctgt taaccttagt tatatccagg gaaatgataa
     1261 acttaaattt gctccaccgg cttttgtttt ccgttataat tcacagaata acttatttga
     1321 acctgccgga gtttcaaacg aaaccaatta tgcaatttca tcctcggtgt cttttcaatg
     1381 gactttacac gaaggggctg ctttaaataa agatatcgtt tcaatttttt ttagcgatga
     1441 tgaagacttt tacatcaatc ttttcagacc ggtaacccgc agcctgtccc ctgctgaaaa
     1501 aaatattaaa atgcatttgc tgattgttgc tgacactgtt gatgcaacca taggaaaagc
     1561 cgtctcaatt gatattacac gggtaactga attattctct tctatttcca actacctggg
     1621 cattaaactg aatacctata ttcttgcagg aaaaagcttt ggcaaatcga atacagaatc
     1681 tgcattgacc aaactaaggc cagcaccaaa cgatattgtc attttttatt attcaggtca
     1741 tggatttcgt attcctgaaa aaccaagggc tttccctaac atgaaactga aaaatgttaa
     1801 aacccccaga acagcattca gggacagcct ggtttgggcc aaagccagcc gtaccgccaa
     1861 tatcagtcaa tcaatgaatg ttgctgatat tttccaaaca attactaaaa aaggtgcaag
     1921 gatgaacctt gtcatcagtg actgttgtaa tgatgatatt ttctccgtga atttcaaaag
     1981 cactaaacct tcaggtacta aaagttcaaa tatcaaatgg agtgagcaaa acattagggc
     2041 cttatttctt gatccaacac cggtatcaat cctggctaca gcatgcacag aaac
//
LOCUS       DAORLS010000438         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_29185_length_2044_cov_1.732026, whole genome shotgun sequence.
ACCESSION   DAORLS010000438 DAORLS010000000
VERSION     DAORLS010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29185_length_2044_cov_1.732026"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>508
                     /locus_tag="PK977_19575"
     CDS             <1..>508
                     /locus_tag="PK977_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20393.1"
                     /translation="ERFAGFKGSVGDHFTYAAKVGFNRLNNQPLFINDTTAGGGGKSF
                     RVVNERQINVLHFGGELGFNIHEKFSLITGLTFNQFSAFKDNAKAWGLIPLELKTTIR
                     MQIIKDLWLKGDLFAWNGPKYMKKDGSVGDLAGATDLNAGLEFKITKNINLWTQFNNI
                     FNREYQRWN"
     assembly_gap    509..555
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            594..>955
                     /locus_tag="PK977_19580"
     CDS             594..>955
                     /locus_tag="PK977_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20394.1"
                     /translation="MHKELFQFLLLHKELPVPGIGTFLLEKISASVDFPNKQIIAPAF
                     RVSLSPDVEVPEKGFFHKLSGLLHQSTKDTVMQFNDFVFNLKKQVMNGAVIDWKGIGI
                     LQKGLSGDIRLIPASDWSL"
     assembly_gap    956..1025
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1026..1242
                     /locus_tag="PK977_19585"
     CDS             <1026..1242
                     /locus_tag="PK977_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20395.1"
                     /translation="EDEKTSTEMTEMLNQPEIKKSYWWVYAMVVGILSVIFIGWHLSE
                     KGLDLNATGNGSKLIPEEVSETYKNLQ"
     gene            1304..>2044
                     /locus_tag="PK977_19590"
     CDS             1304..>2044
                     /locus_tag="PK977_19590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF20396.1"
                     /translation="MSQRVHYTNCPVCGAFGIEEKLIVTDFTVSKEKFPIALCHACTA
                     MFTQNIPDVSSIGPFYKSEEYISHTNTSKGLINRLYKSVRRRTIRQKIKLIENVTGLK
                     QGSLLDVGSGTGVFASVMQQHGWQVTGLEPDPGARKVANEVNGIELGDINSFYQLPNN
                     SYDAITLWHVLEHVHDLQGYMTKLKLLLKEKGKLFIAVPNYTCKDAAVYREYWAAWDV
                     PRHLYHFSPVSMEKLVEKHGMRIEAYKPM"
BASE COUNT          588 a          335 c          443 g          561 t
ORIGIN      
        1 agaaagattc gctggtttta agggctcggt cggagatcac ttcacttatg cggcaaaagt
       61 tgggtttaac aggttaaata atcagccttt gttcatcaat gacacgacag ctggtggcgg
      121 gggtaaatca ttcagagtag tgaatgaaag gcaaataaat gtgttgcatt ttggcgggga
      181 acttggattc aacatacacg aaaagttctc tttaattact ggactgacat ttaaccagtt
      241 ttcagctttc aaggataatg caaaggcatg gggacttatt cctttggaat tgaagactac
      301 cattagaatg cagatcatta aggacctttg gctgaaaggt gacctgtttg cctggaatgg
      361 tcctaagtac atgaagaagg atggaagtgt tggagatttg gctggagcca ccgatttaaa
      421 tgccgggctt gagtttaaga tcaccaagaa tataaacctc tggacacagt tcaataatat
      481 tttcaacagg gaataccagc gctggaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnncggcg gaatcgtatt ttcgttcgac caaaagaact gagatgcaca
      601 aagaactgtt tcaatttctg cttttacata aagaattgcc agtgccgggt atcggcactt
      661 ttttattgga aaaaatttca gcttccgttg attttcctaa taaacaaata atagcccccg
      721 ctttccgggt aagcttaagc ccggatgttg aagtgcccga aaaaggtttc ttccataaat
      781 tgagcggatt acttcatcaa tcaacaaagg atactgttat gcagttcaat gattttgttt
      841 ttaatctaaa gaagcaggtt atgaacggcg ctgttattga ctggaaaggt ataggcattt
      901 tacaaaaggg cctgtctggt gatattagat tgatacctgc ttcagattgg tcgctnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnggaag atgaaaagac ttcaactgaa atgaccgaga tgctcaatca gccggaaatt
     1081 aagaaatcat actggtgggt atatgccatg gttgtcggaa tactgtcagt aatattcatc
     1141 ggctggcatt tatcagaaaa agggctggat ctaaatgcaa cgggtaatgg atcaaaatta
     1201 attccggaag aggtcagtga aacctataaa aatctccaat agagatcctt tttcatcatt
     1261 ttaattttac tttgcctgat cccattttac aggagaatta ttgatgagtc aacgagtgca
     1321 ttatacaaat tgcccggtat gcggagcttt cggaattgaa gagaagctta tcgttactga
     1381 ctttacagtt agtaaggaaa agtttcccat tgcactctgt catgcatgca cagccatgtt
     1441 tacacagaat atccctgatg tttcttctat tgggcccttt tataaatcgg aggaatatat
     1501 ttcacataca aatacttcaa aagggttgat caatcgctta tacaagtcag tacgaagaag
     1561 aacaatccgt cagaaaataa agctgattga aaatgtaaca ggtctgaagc agggctcact
     1621 gcttgatgtt ggaagcggta caggggtttt tgcttcagtg atgcaacagc atggatggca
     1681 ggtaaccggc cttgaaccgg atcctggcgc ccgaaaagta gccaatgaag tcaatggcat
     1741 tgagttggga gatattaaca gcttttatca attgcccaat aactcttatg atgctattac
     1801 gttgtggcat gtattggagc atgtacatga tctgcagggg tatatgacaa aattaaagtt
     1861 attgctaaag gagaagggga aattatttat tgccgttccc aattacacct gtaaagatgc
     1921 ggctgtttat cgtgaatatt gggcagcatg ggatgtaccc cggcatctgt atcatttttc
     1981 tccggtatca atggagaaat tggttgaaaa acatggtatg cgtattgaag cttataaacc
     2041 tatg
//
LOCUS       DAORLS010000439         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_29205_length_2043_cov_1.574447, whole genome shotgun sequence.
ACCESSION   DAORLS010000439 DAORLS010000000
VERSION     DAORLS010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29205_length_2043_cov_1.574447"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PK977_19595"
     CDS             <1..748
                     /locus_tag="PK977_19595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF20397.1"
                     /translation="IREKAENSIIQELIGIVAAFPSVNSIRVLDDLFLKTNATVQKAI
                     NVFSHFRLQWRSMAHVMTFNNVDDSVMSQLKDSGCYELFIGIESGSPKILGEINKTKN
                     VDTILNNLTKVFKAGINMKGYFIYGFPGETQEDMEMTFQLAVKLKEISIINGVTFRTS
                     VFQYRPYHGTQIYHDLEIKGENLKVQQIQPNQGLSELVGRLQFNFHSGNYSKVDDKLL
                     HNYIYRTINLNDGKIFAGLESKNQTLPQGM"
     gene            711..1304
                     /locus_tag="PK977_19600"
     CDS             711..1304
                     /locus_tag="PK977_19600"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRF20398.1"
                     /translation="MNQKIKPCRKVCKTCGLYINQLPVLDFLKQSHIFWVGLSAVQFD
                     EGVEHLPLSPTTRTGALINTIEQPLIDNIGFYKTNLVKCVPLKENGKIRYPIEHEMEK
                     CYPNFQFELEALKPSVVFLLGKQVSAFIMKKMGVKDVYLPDNFRYKALEIGNTFFVPV
                     HHPSFMLVYKRKQLSIYIKSIQSVCRDKTKNKLLQTA"
     gene            complement(1564..1803)
                     /locus_tag="PK977_19605"
     CDS             complement(1564..1803)
                     /locus_tag="PK977_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20399.1"
                     /translation="MKKILVAILAVGLISSLAIAANGGGKKKGKKKAKMECKQDKNCD
                     VKNCVPPNCDPTVCPYPVCEKDEKCPTEKNCSGKK"
     gene            complement(1846..>2043)
                     /locus_tag="PK977_19610"
     CDS             complement(1846..>2043)
                     /locus_tag="PK977_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20400.1"
                     /translation="EKEEDECAGNRCNPLLSCPIGNFYLSGYTQLTIPPYVAEKVKTF
                     LVNDNRIVKQHADCWHPPEIS"
BASE COUNT          632 a          377 c          388 g          646 t
ORIGIN      
        1 cattcgtgaa aaagctgaga attctataat acaggaattg attggaatag ttgctgcatt
       61 cccttctgtg aactcaataa gggtgttgga tgacctattt ttaaaaacaa atgcaactgt
      121 acagaaagct ataaatgtat tttctcattt tagacttcag tggcgttcca tggcccatgt
      181 aatgactttt aacaatgttg atgactctgt tatgagccaa ttaaaagaca gtggatgtta
      241 tgaacttttt attggaatag aatcgggttc gccaaaaata ttaggtgaaa ttaataaaac
      301 taaaaatgta gatacaatcc tgaataattt aactaaagtc tttaaagcag gtataaatat
      361 gaagggctat tttatttatg gctttccagg agaaacgcaa gaggatatgg aaatgacctt
      421 tcaattggca gttaaattaa aggaaatctc tattataaat ggggttactt tccgtaccag
      481 tgtatttcaa taccggcctt atcatggaac tcaaatctat catgacctgg aaataaaagg
      541 cgaaaattta aaagttcagc aaatacagcc aaatcaagga ctgtcggagc ttgtaggacg
      601 gctgcaattt aattttcata gtggcaatta ttcaaaagtt gacgataagc tgcttcataa
      661 ctatatttac cgcacaatca acctgaatga cggtaaaata ttcgctggac ttgaatcaaa
      721 aaatcaaacc ctgccgcaag gtatgtaaga cctgtgggtt atacattaat cagttaccag
      781 ttcttgattt tctaaagcaa tcacatattt tttgggttgg tttatccgct gtacaatttg
      841 atgaaggtgt tgaacatctt cctttatcac ctacaacccg aacgggagca ttaataaaca
      901 ctattgaaca gcctttgatt gataatatcg gtttctacaa gacaaatctt gtaaaatgtg
      961 ttccattgaa agaaaatgga aaaatcagat acccgataga gcatgaaatg gaaaagtgtt
     1021 accccaattt tcaatttgaa ctggaagctc ttaaaccatc ggttgttttt ttattaggga
     1081 aacaagtgtc cgcttttata atgaaaaaaa tgggagttaa agatgtttat ttgccagata
     1141 atttcagata taaggcactt gaaataggta atactttttt tgtgccagtt catcatccat
     1201 cttttatgtt ggtctacaaa agaaaacagc ttagcatata cattaaaagt attcagtcag
     1261 tttgccggga taaaaccaaa aataaacttc ttcaaactgc ataaactgtt cccccgttat
     1321 ccggagtctt cgggttaata ataactcttg aactgtagga tagttgtgct gagactgctt
     1381 ctcagtaccg gctttgccaa aagccgtcaa aaagttgcag ttatattcct taatgcacct
     1441 ctcccttgcc ggttttgcag caggctaatt cccgggcatt gctcaatgca accactaata
     1501 tccactgcaa taaaaaaagc cacctcctta cggaagtggc ttaaatgttt atgacaacat
     1561 tttttatttc ttacccgaac agttcttctc tgtcggacat ttttcgtcct tttcacaaac
     1621 agggtaagga caaactgtag gatcgcaatt gggcggaaca cagtttttta cgtcacagtt
     1681 tttatcctgt ttgcattcca ttttggcttt cttctttccc tttttcttgc cgccaccgtt
     1741 tgcggctata gccagggaag aaataaggcc gacagccagt atggctacga gtattttttt
     1801 catattgtat tagttgatga gtaaatatat tcattagctg tgtaattaac tgatctcggg
     1861 cgggtgccag caatcggcat gctgctttac aatgcggtta tcatttacca gaaaggtctt
     1921 tactttttct gctacatacg ggggaatggt cagctgggta tacccggaca aatagaaatt
     1981 cccgataggg catgatagca gcgggttgca gcggttacct gcacattcat cttcctcttt
     2041 ttc
//
LOCUS       DAORLS010000440         2042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_29223_length_2042_cov_1.718671, whole genome shotgun sequence.
ACCESSION   DAORLS010000440 DAORLS010000000
VERSION     DAORLS010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29223_length_2042_cov_1.718671"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PK977_19615"
     CDS             <1..449
                     /locus_tag="PK977_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20401.1"
                     /translation="ASWQSLPATVAFKQVGANGNLNVNAGTSVDINTWNVNEYNLGGG
                     TYNAATGEYTVPVTGLYQVFAKTKTFGNSGTGFYNMNIKVNGIIVSAHSVPVSTNGII
                     QADANSAIQLNAGDLVKIEFQNSATNNTTIPNWVTIDMEFSVNRIK"
     gene            467..1009
                     /locus_tag="PK977_19620"
     CDS             467..1009
                     /locus_tag="PK977_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20402.1"
                     /translation="MKKIILSGVALLAVLAGFSQSNSTVQNSGDAANLERIRQNQIKN
                     KTVEEVAKPVKSTPVAPDIAAPGVVNPARPVVNKMPAPTGNMQNGSIVTEPTAKPASI
                     QKIENPETKPKPEVVAPKTDGAKSLTNKEALPSAAKGSSVDPDAKVVAPVKQTDTPVV
                     VPTQNSGNTAPAIEKPVKKE"
     gene            1449..>1681
                     /locus_tag="PK977_19625"
     CDS             1449..>1681
                     /locus_tag="PK977_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20403.1"
                     /translation="MRRRVTLSLLAIFMAIGFCKAQVPSVVPWNGVVPAIMNTAGGTY
                     DNALSYYRYEWSFGELVLIQAFAPPDSSILVTQG"
     assembly_gap    1682..1702
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1703..>2042
                     /locus_tag="PK977_19630"
     CDS             <1703..>2042
                     /locus_tag="PK977_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20404.1"
                     /translation="DKIGVSPYTVLFEEGDYKLFPNPTSGKFEVDFFVKTPGFMSLQL
                     INSIGQILETRSYHYDGCCRIEMFDLTRYPNGIYFVVADLRPDTRRPSTIAGGDIEVI
                     RHSGLKVIKMD"
BASE COUNT          606 a          405 c          441 g          569 t
ORIGIN      
        1 atgctagctg gcagagtctt cccgctactg ttgcttttaa acaagtggga gctaatggca
       61 acctgaatgt gaatgctggt acaagcgttg atattaacac ctggaatgta aatgagtata
      121 atttaggtgg aggtacttat aatgctgcca caggtgaata tactgttcct gttacaggtc
      181 tgtaccaggt atttgcaaaa acaaaaacat ttggtaattc gggtacgggt ttttataaca
      241 tgaatatcaa ggttaatggg ataattgtgt ctgcacattc agtacctgtt tcaaccaatg
      301 gtattattca ggctgatgcc aactctgcga tacaattaaa tgccggtgac cttgttaaga
      361 tagaatttca gaattctgca accaataata cgacaattcc taactgggta acaattgaca
      421 tggagttcag tgttaacaga attaaataac ttataaaatc aatgaaatga aaaaaattat
      481 tttaagtgga gttgcgttgt tagcagtgtt ggccgggttt tctcagtcaa actccaccgt
      541 acaaaatagt ggtgatgcag ccaatttgga aagaataagg caaaaccaaa taaagaacaa
      601 aacagtggaa gaggtggcta agcctgtaaa aagtactccg gtggcgccag atattgcagc
      661 cccgggggtg gttaatccgg ctcgtccggt cgtaaataag atgcctgctc ctacgggcaa
      721 tatgcaaaat ggaagtattg ttaccgaacc tactgcaaaa cctgcctcaa tacagaaaat
      781 tgaaaacccc gaaactaaac ctaaaccaga ggtagtggca ccaaaaaccg acggtgcgaa
      841 atcgctgact aacaaagaag ccttaccatc tgctgcaaaa ggcagttctg ttgacccgga
      901 tgcaaaggtt gttgcaccag taaagcaaac tgatactccg gttgtggttc cgactcaaaa
      961 ttccggaaat actgctccgg ctattgaaaa acctgtgaaa aaagagtgag acttcaatag
     1021 caagtttaag caagaaggcc ttcgggcctt cttttttgtt taataagttg cttaataggt
     1081 cagtgtagtt tctaatactg gtatggcttg ctgactgccc ggttaatatc gcatgaagac
     1141 gatcatattt cccctttgaa aagaaagttg ataaagtttt gccagaatga tattgcaaaa
     1201 gggaaactat tggtgtatta ttaaatgatg tttcattatg aatatattat actagacttt
     1261 aattatcagt tgattaaaca attagatttt ataaaataaa tttctttcat tcctgtaact
     1321 tttctacatt cgttattgta ttccatacag gatattatct ctggttaatc tctctcttta
     1381 gtggcatagc ccatcttcgg gcatccaacc ccctctgaaa tattttataa ccattaaact
     1441 cagcgcttat gagaagaaga gttactcttt ctctgttagc catattcatg gctatcggct
     1501 tctgtaaagc acaggttcct tctgtagttc catggaacgg ggtagtaccg gcaattatga
     1561 acacggccgg cggtacctac gacaatgcgt tgtcgtatta ccgttatgaa tggagctttg
     1621 gagaactggt gctgatacag gctttcgccc ccccggacag ttcaatttta gtgactcagg
     1681 gnnnnnnnnn nnnnnnnnnn nngacaagat cggtgtatca ccgtacacgg tgctgttcga
     1741 agaaggcgat tacaaactat tccctaatcc tacatccggc aaattcgagg tggacttttt
     1801 tgtaaagaca cccggattca tgagccttca gctgatcaat tccatcggtc agatactgga
     1861 gaccaggagc tatcactatg atggctgctg ccgtatcgaa atgttcgacc tgacaaggta
     1921 ccccaacggg atctactttg tagtggccga cctgaggccg gatacccgtc gccccagcac
     1981 tattgccggt ggtgatatag aggtaatccg ccacagtggc ctaaaggtga tcaaaatgga
     2041 tt
//
LOCUS       DAORLS010000441         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_29831_length_2008_cov_1.802867, whole genome shotgun sequence.
ACCESSION   DAORLS010000441 DAORLS010000000
VERSION     DAORLS010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29831_length_2008_cov_1.802867"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PK977_19635"
     CDS             complement(<1..475)
                     /locus_tag="PK977_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF20405.1"
                     /translation="MKKIIAAFDGLKYAENTAACALHLAKRTQTHLVGVFPDDMTYTS
                     YLIHELVVKKGITADQLKKYEERDAKSRKQSVEKFEKVCKQEGIQYSLHQDKKVAIQC
                     LKHESIYADLLVISMKETFSHYPEKPPTRFIRDLLSDTQCPVLLVPEKYNGFEKVV"
     gene            complement(579..>795)
                     /locus_tag="PK977_19640"
     CDS             complement(579..>795)
                     /locus_tag="PK977_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20406.1"
                     /translation="YHHVTTGYTESLKQLYKETTAGHVNESEITTLLNFNREIFTAFK
                     SFVFGIKDCLFNREQAKYFDELPGFIR"
     assembly_gap    796..812
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<813..>2008)
                     /locus_tag="PK977_19645"
     CDS             complement(<813..>2008)
                     /locus_tag="PK977_19645"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRF20407.1"
                     /translation="QNALAVILGANLGTTVTGWLVALAGFNVNIENLALPVAGIAGLG
                     FAVFDRNGKWYNWSRFLLGFSFLFIGLGYMKTGIEGLVLNLDLRQYANAPLVTFLLIG
                     FLITSLIQSSSATVAITLSALYAGAITLVDGVAIVLGSEVGTTVKLFMASIKGESAKK
                     RVALGNFLFNIITAALILIFLLPLTNFVTDVIGIKDELIALVFFQTFVNILGIILFYP
                     LLGIFGRFLENRFLNDNDETMFIHKVASKEPSTALLALESESRHFIYSVIDFTRSSFS
                     LANPDIARMKLHKEFLGKSIPEKYEYIKFLHGEIHSYCIQLQKNLVDKEDIEKLNRLI
                     SAVRNTMYAAKSIKDALPDINQLSNSSNDVKFGFYTQSRGGVDEFCTKAFHIVDENPD
                     LHFEDL"
BASE COUNT          620 a          444 c          351 g          576 t
ORIGIN      
        1 aaacgacttt ttcaaatcca ttatactttt ccggcaccaa taacaccggg cactgtgtat
       61 cactgagcag atccctgata aaacgggtag gtggcttctc aggataatga gaaaacgttt
      121 ccttcatact gataacaagt aaatcggcat aaatgctttc atgcttcaaa cattgtatcg
      181 ctactttttt gtcctggtga agtgaatact gaataccttc ctgtttacaa actttttcaa
      241 acttttctac agactgctta cgggattttg catccctctc ttcatacttt tttagctgat
      301 cggctgtaat tccctttttt actaccaatt catgaataag gtagctggta tacgtcatat
      361 catcaggaaa cacacccacc aggtgtgttt gtgtacgttt tgcaaggtgc aatgcacaag
      421 cagcggtatt ttccgcatac ttcaaaccgt caaatgcagc tatgatcttt ttcatacaca
      481 agaattttaa aaacagttgg ctgttcacag ctaaactatt caccttctcc aggttaacca
      541 atgacgggta ttagcagtat caatgacttt ggtcaaaatc aacggatgaa accaggaagc
      601 tcatcaaaat actttgcctg ttccctgtta aacaaacagt cttttatacc aaatacaaac
      661 gacttgaaag cggtaaaaat ctccctgtta aagttgagaa gggttgttat ctccgattca
      721 ttaacatggc ctgcggttgt ctccttgtag agctgcttca gcgattcggt atagccggtt
      781 gttacatgat ggtaannnnn nnnnnnnnnn nnaagatctt caaaatgcag gtccgggttc
      841 tcatccacaa tgtggaatgc ttttgtgcaa aactcatcaa cccctcctct tgactgagta
      901 taaaatccaa atttcacatc attggatgag tttgataact ggttgatatc cggcagcgca
      961 tctttaatac ttttagcagc atacatagta ttcctcaccg cagagattag ccggttcagc
     1021 ttttcgatat cttccttgtc cacaaggttc ttctgcaact gaatgcaata actgtggatt
     1081 tcaccatgca agaactttat gtattcatac ttttcaggaa tgctttttcc caaaaattct
     1141 ttatgaagct tcatcctggc aatatctggg tttgccagac taaaagaact tcgtgtaaaa
     1201 tcaattacac tgtaaataaa atgacggctt tcactttcaa gtgccagcaa tgccgttgat
     1261 ggctccttcg aagctacttt atgtatgaac atcgtttcgt cattatcgtt caaaaagcga
     1321 ttttcaagga aacgtccgaa aatcccaagc aaggggtaaa acaagatgat gcccagtata
     1381 ttcacaaatg tttgaaaaaa aacaagagca atcagttcat ctttaatgcc gataacatca
     1441 gtaacaaaat ttgtcaaagg cagaaggaag atcaatatta aagcagccgt aatgatattg
     1501 aataaaaaat ttcccaatgc cacccttttc tttgcagatt caccctttat actggccata
     1561 aacaatttga cagtggtgcc cacttctgaa cctaatacaa tagctacacc atccaccaga
     1621 gtaattgctc ctgcatacaa ggcgcttaaa gtaatagcaa cagtagcaga actactctga
     1681 ataagcgatg taatcaggaa tccaatgaga agaaatgtca ccaatggcgc attggcatat
     1741 tgtcttagat cgaggttcag taccaggccc tcaataccag ttttcatata accaaggcct
     1801 ataaaaagaa aactaaatcc taataaaaaa cggctccagt tataccattt accatttctg
     1861 tcgaaaacgg caaagcccag cccggcaata cctgcaacgg gtaaagcaag gttctcaata
     1921 ttgacattaa agccggccag cgccaccagc cagcctgtta ctgtagtacc cagattggct
     1981 cccaatatta cagcaagggc attttgca
//
LOCUS       DAORLS010000442         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30001_length_1999_cov_1.706790, whole genome shotgun sequence.
ACCESSION   DAORLS010000442 DAORLS010000000
VERSION     DAORLS010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30001_length_1999_cov_1.706790"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>248
                     /locus_tag="PK977_19650"
     CDS             <1..>248
                     /locus_tag="PK977_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20408.1"
                     /translation="EETLNKELLFFNELSLDKARQLVAAKTWEITKVVPIKSNTKYWR
                     YVVAAAVIVAGFFAIYQYSMKSGSDRESVKTTTEKPFI"
     assembly_gap    249..258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <259..1041
                     /locus_tag="PK977_19655"
     CDS             <259..1041
                     /locus_tag="PK977_19655"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRF20409.1"
                     /translation="GNKATLQLADGSVIVLDSAANGNLTSQGNVKVIKLDGKLTYNSS
                     NNRSEAVYNTIKTPRGGQYQLVLADGSQVWLNAASSIRFPVDFTEKVRKVEITGEAYF
                     EIKKDAAKPFIVTIPGKGQVEVLGTHFNINAYDDENTLNTTLIEGSVKFIADNGESVK
                     LKPGQQAQLSSSVSVLNDADIDKIIAWKTGWFNFDRADITTIMRQVSRWYDVEVVYEG
                     QVSKKTFSGIVSRSQQIAEVLKIMEKAGVKFRIEGKKITVLR"
     gene            1185..>1652
                     /locus_tag="PK977_19660"
     CDS             1185..>1652
                     /locus_tag="PK977_19660"
                     /inference="COORDINATES: protein motif:HMM:NF019283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HRF20410.1"
                     /translation="MDFNIQGFPAPKPGNRIKTSLQVRQALLIMKFTAVLLLAAFLQV
                     SAKGTAQKVTLSEKNVSLQKIFKQINKQTGYQFFYEDDLLDQAGKVDISIKNGELKEV
                     LESCFKNLPITYEIVNNTIIVKAKSKILNEEEKLLPPPPPPPITVKGLVRTENG"
     assembly_gap    1653..1710
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1711..>1999
                     /locus_tag="PK977_19665"
     CDS             <1711..>1999
                     /locus_tag="PK977_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20411.1"
                     /translation="TTGVNGTFTLEGIDEKAVLVFTGVNIETQEVALNGKTSVVVTVK
                     IRVSPLDEIQIIGYGKTTRRYKTGAVTTLKADAIDRQPVANPLQALQGRMAG"
BASE COUNT          673 a          343 c          444 g          471 t
ORIGIN      
        1 atgaagagac cctgaacaaa gagcttcttt tttttaatga actttcacta gacaaagcca
       61 ggcaattggt tgcagctaaa acctgggaaa tcaccaaagt agtacccatt aaaagcaata
      121 caaaatactg gaggtatgtt gtggcagcag ctgttatagt agcaggtttt tttgccattt
      181 accagtatag catgaagtct gggtctgata gggagtctgt taaaacaaca actgaaaaac
      241 catttatann nnnnnnnngg aaacaaagcc acacttcaat tggcagatgg tagtgtgata
      301 gtactggaca gcgccgccaa tggcaacctt acaagtcagg gcaacgtaaa agtgataaag
      361 ctggatggaa aacttacgta caacagcagt aacaaccgta gcgaagcagt gtacaatact
      421 atcaaaacac ctaggggggg gcagtatcag ttggtattag ccgatggctc acaggtgtgg
      481 ctgaatgcag catcttctat tcgctttccg gttgatttta ccgaaaaagt tcgcaaagtg
      541 gagattacgg gtgaagccta ttttgaaata aaaaaagatg cggctaaacc tttcattgta
      601 actattcctg gtaaaggtca ggtggaagtc ctgggtacac attttaatat aaatgcttat
      661 gacgatgaaa acactctgaa tacaacattg attgaaggaa gtgtaaaatt tattgctgat
      721 aacggcgaat ctgtaaagct taaacccggc cagcaggcgc aattatcatc atcggtttca
      781 gtgttaaacg atgcagatat tgataaaatt attgcatgga aaaccggatg gttcaatttt
      841 gaccgggccg atataacaac gatcatgcgg caggtaagcc gatggtacga tgtggaggtt
      901 gtgtatgaag ggcaagtatc aaagaaaacc ttttctggta ttgtaagcag aagccaacaa
      961 atagccgaag tgctgaaaat aatggagaaa gccggtgtga agttcaggat tgaaggtaaa
     1021 aagataacag tactccgtta ataactgagt gatcatagcg gccaataaca aaaccgtcct
     1081 gaggtcgagt caggacggcg aagtaagggc tgcacaatta ttatagcaaa cactgcctga
     1141 aagcagtaaa aacagaattg tttaacccaa accagaccaa aattatggat tttaacattc
     1201 aaggttttcc ggctccaaaa ccggggaacc ggatcaaaac cagtctgcag gtaaggcagg
     1261 cattgctgat tatgaagttt accgctgttt tattgctggc tgcctttttg caggtaagtg
     1321 caaaaggaac tgcgcaaaag gtaacattat cggaaaaaaa tgtttccctg cagaagattt
     1381 ttaagcagat aaacaaacaa accggttacc agtttttcta tgaagatgat ctgctggatc
     1441 aggcaggtaa agtagatatt tcaataaaaa acggtgagtt gaaagaagtg ctggaaagtt
     1501 gttttaaaaa tttacccatt acatacgaga tagtcaacaa taccattatt gttaaggcga
     1561 aaagtaagat attaaatgag gaagagaaac tattaccacc cccgccccct cctccaataa
     1621 cagtgaaagg attagtaaga acagagaatg gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caacaacagg agttaacgga acttttactc
     1741 ttgaaggtat agatgaaaaa gctgtgctgg tttttacagg tgtgaacatt gaaacacagg
     1801 aagtggcctt aaatggtaaa acatcagtag tagtaaccgt aaagatccgt gtatccccat
     1861 tagatgaaat acaaataatt ggatatggta aaactacacg gagatataaa acgggagctg
     1921 ttacaacact aaaagcggat gcgatagacc gtcagccagt tgccaatccc ctgcaggcat
     1981 tacaaggcag aatggccgg
//
LOCUS       DAORLS010000443         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30034_length_1997_cov_1.895469, whole genome shotgun sequence.
ACCESSION   DAORLS010000443 DAORLS010000000
VERSION     DAORLS010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30034_length_1997_cov_1.895469"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1209
                     /gene="mltG"
                     /locus_tag="PK977_19670"
     CDS             172..1209
                     /gene="mltG"
                     /locus_tag="PK977_19670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRF20412.1"
                     /translation="MRKKIIFGMLLLILAVGSFLGYKLFTPAVHNKDNAYFYIKDGDD
                     VASVKETLVYQQFIGGSGFDLAARLLKFKKARSGRYKLKDGMSMYQLIKLLRSGEQSP
                     VKVVITKERTKEAFAGKFGKRFDVSFDSLAMIRFLSSNDSLKDYGVDSNTVMAVIMPY
                     TFEVNWNSTPEKVFKQFYASFKKFWTPERKTKADSLHLTPLQVITLASIVEEETLKKA
                     DKYNIASTYLNRIRTGMKLQADPTVKFALKDFTIKRVTGTHLKKDSPYNTYMYAGLPP
                     GPICTPSIETIEAVLDAPETEYLYFVASSKFDGSSVFTTNFDDHLKYARLYQQELTRR
                     MDSARKAKENK"
     gene            1209..>1997
                     /locus_tag="PK977_19675"
     CDS             1209..>1997
                     /locus_tag="PK977_19675"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRF20413.1"
                     /translation="MPFITKIVHKIANSGPAKFIIDKSKETSLPGFRGIPIYDVVNFF
                     IGQVQTIGMTERASSIAFNFVMAIPPAIIFIFTLIPLLPITDQFQEQLFDLIRDVIPG
                     EKEKPVLVNFLKDFINNPRNGLLSLGFLLSMFFSSNAMMGIMRSFDKNYIGFRKRKGF
                     QKRLVAIKITMFMYIIVFVSVLLLTGREFVLEYLGIKNETARAIILNARWLVIILLFF
                     ACISFIYRHAPAVHKKWRLIKPGSILATFLMLLMTLLFSWWVGQF"
BASE COUNT          596 a          433 c          415 g          553 t
ORIGIN      
        1 aggactaagg atgctactac tgcccctatc ccgtccacta accagcctgc acccgctaat
       61 aacaataaca ccattcagtt gaacacacaa cctgacacca cgaaataaaa tacttctata
      121 atattggcga accctgtctt accgacaggg ttttttattt attttgtttc catgaggaag
      181 aaaataattt tcggcatgct gctgctgata cttgcagtgg gtagtttcct gggttataaa
      241 ttatttacac ctgcagtaca taacaaggac aatgcttatt tctatataaa ggatggtgac
      301 gatgttgcct cggtaaaaga aaccctggtt taccagcaat tcattggtgg cagcggattt
      361 gaccttgctg cccgtctcct aaaattcaaa aaggccaggt ccgggcgata caagctgaaa
      421 gacggcatga gtatgtacca gctcatcaaa ctgctgagaa gcggagaaca gtcacccgta
      481 aaagtggtaa tcaccaaaga aagaacaaaa gaagcttttg ccggaaaatt tggaaaacgg
      541 ttcgatgtat cttttgactc actggcgatg atccgttttc tttccagcaa tgattcgcta
      601 aaagactatg gagtggacag caacaccgtt atggcggtga ttatgcccta tacttttgag
      661 gtcaactgga attccacacc ggaaaaggta tttaaacagt tctatgcatc ctttaaaaaa
      721 ttctggacac cggaaagaaa aacaaaagcc gacagtcttc atttgacacc gctgcaggtt
      781 attaccctcg cttcgattgt agaagaagaa acgctgaaaa aagcagataa atacaatatt
      841 gccagcacct atctgaaccg catacgcacc ggcatgaaat tgcaggcaga tccaactgta
      901 aaatttgccc tgaaagattt caccattaaa agggtgacgg gtactcacct gaaaaaagat
      961 tcgccataca atacatatat gtatgcgggt ttgccccctg gccctatctg cactccttct
     1021 attgaaacaa ttgaagccgt actggatgct cctgaaacag aatacttgta ttttgtagcc
     1081 agcagcaaat ttgacggcag cagcgttttc accactaact ttgatgacca cctgaagtat
     1141 gcaagactgt accagcagga actaacgagg cgaatggact ctgccaggaa ggccaaagag
     1201 aataaataat gccttttata accaaaatag tacataaaat agccaactcc gggccggcca
     1261 aatttatcat tgacaagagt aaggaaacat cactgccggg attcagggga atacctatct
     1321 atgatgtggt taattttttc atcgggcagg tgcaaacgat cggcatgacg gaaagggctt
     1381 cctctattgc tttcaatttt gtgatggcca taccaccggc catcattttc atttttacat
     1441 taattccttt gttgcctatc accgatcagt tccaggagca gttatttgac cttataaggg
     1501 atgtcatccc gggagaaaaa gaaaaaccgg tgctggtaaa cttcttgaaa gattttatta
     1561 ataatcccag aaacggcctg ttgtcattgg gttttttgtt gtccatgttt ttctcttcca
     1621 atgccatgat gggtatcatg cggtcgtttg acaaaaacta tatcgggttc agaaaaagaa
     1681 aaggttttca aaaacggctg gtagctatta agatcaccat gttcatgtac ataattgtgt
     1741 ttgtttcagt tttgctgctg acaggacggg aatttgtgct cgaatatctc ggtatcaaaa
     1801 atgaaacggc cagggcaatt atcctgaatg cccggtggct ggtgatcata cttttattct
     1861 ttgcctgcat ctcctttatt tacagacatg ccccggctgt acataaaaaa tggcggctga
     1921 tcaaacccgg ctccatatta gcaacttttc tcatgttatt aatgaccctg ctattttcct
     1981 ggtgggttgg ccagttt
//
LOCUS       DAORLS010000444         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30168_length_1990_cov_1.565891, whole genome shotgun sequence.
ACCESSION   DAORLS010000444 DAORLS010000000
VERSION     DAORLS010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30168_length_1990_cov_1.565891"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK977_19680"
     CDS             <1..591
                     /locus_tag="PK977_19680"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRF20414.1"
                     /translation="SLEAMTYGAKVAEQLGTTAEAVVLGAVNDDLGALGKYGVKKIHH
                     VAADALNHFDAQVFTKAIAEVANATGAKVIIFSNNISGKAIAPRLSVRLKAGLVSGAV
                     ALPDTSNGFVVKKNVFSGKAFANVAVNTDVKIIALSPNAYQTIAGEGSAEVVSASTTA
                     TAGKVKVTAVNKASGEVPLTEAEIVVSAGRGMKGPE"
     gene            609..>877
                     /locus_tag="PK977_19685"
     CDS             609..>877
                     /locus_tag="PK977_19685"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF20415.1"
                     /translation="MHAATACSRPVADAHWRPHNEHVGQTGFAIAPNLYIAIGISGAI
                     QHLAGVNRSKVIVVINKDPEAPFFKAADYGIVGDAFDVVPKLTAA"
     assembly_gap    878..974
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <975..>1246
                     /locus_tag="PK977_19690"
     CDS             <975..>1246
                     /locus_tag="PK977_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20416.1"
                     /translation="KLVNLFLVLLIANTLPAQKDFEGCITYKVTQKLESDNGDTPEEI
                     NIWFGHGRIRVNTIKGGTEEYLLLAIDSGKAYSLNKTSKTYEVNTL"
     assembly_gap    1247..1263
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1264..1796
                     /locus_tag="PK977_19695"
     CDS             <1264..1796
                     /locus_tag="PK977_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20417.1"
                     /translation="KTRKKYAVAEKEVIAGHIATPVQSGTNLWGSSFGMSSILWFSDS
                     LLFNVPEKFEGNEELLMVHNNHILLKARIVMDLNLRGNSFNDESEPSDSSDENYFVLE
                     AIKVMPQPTAPGFFTIPDDYTKLTLLAMPDTAAIMVDTAMAEEIEPPPPPAPKKAPAK
                     TPVKGKTYKSSLRKEN"
BASE COUNT          574 a          416 c          415 g          471 t
ORIGIN      
        1 tcgttggaag caatgaccta tggcgccaaa gttgcagagc aactgggtac cactgcggaa
       61 gccgttgtgc tgggtgcagt caatgatgac ctcggtgctc tcggcaaata cggtgtcaaa
      121 aagatccatc atgtagctgc agatgcactc aatcattttg atgcacaggt tttcactaaa
      181 gctatagctg aagtggccaa cgctactggt gccaaagtga ttattttctc caataatatc
      241 agtggtaaag ccattgctcc ccgcctttca gtccgtttaa aagcaggatt ggtttccggt
      301 gctgttgctt tacctgatac aagcaatggt tttgtagtaa agaaaaatgt tttctccggt
      361 aaggcctttg ccaacgttgc agtcaatact gatgttaaga tcattgccct gagcccgaat
      421 gcttatcaaa caattgccgg tgaaggaagt gcagaagtag tttcagcctc tactaccgct
      481 actgcaggca aggtcaaagt aacggccgtg aacaaagcca gtggtgaagt gccgctgaca
      541 gaagcagaaa tcgttgtaag tgccggccgc ggtatgaaag gacccgagta gaagaactgg
      601 ccaaaacatt gcatgctgcc actgcctgca gccgccctgt tgcagatgca cactggcgtc
      661 cgcataatga acatgtgggt caaaccggct tcgccattgc acccaacctg tatatcgcta
      721 ttggtatctc aggagctatt cagcaccttg ctggtgtaaa caggagtaaa gtgatcgtgg
      781 taatcaataa agatcctgaa gcgcctttct ttaaagctgc cgactatggc attgtgggtg
      841 atgcatttga tgtggttcca aaactcaccg ctgctatnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnngaaatt agtgaattta ttcctggtac ttcttattgc aaatacactg
     1021 cctgcccaaa aagattttga aggttgtatt acttataagg taacacaaaa attagaaagt
     1081 gataacgggg acaccccgga agaaattaat atctggttcg gacatggccg cataagggtg
     1141 aacaccatca aaggcggaac tgaggaatat cttctcctgg cgatcgattc aggaaaggct
     1201 tacagcctga acaagaccag taaaacctat gaagtaaata ctttaannnn nnnnnnnnnn
     1261 nnntaaaaac ccggaaaaaa tatgctgtag cagaaaaaga agtaattgcc ggtcatattg
     1321 ccactcccgt acaaagcggt actaacctgt ggggcagttc ctttggcatg agttctatac
     1381 tctggttttc agacagcctg ctcttcaatg tgcctgaaaa atttgaaggc aacgaggaac
     1441 tgctgatggt gcataataac catatacttc tcaaggcacg gattgttatg gatttaaatt
     1501 tacggggcaa cagtttcaat gatgaaagcg aaccttccga tagcagtgac gagaattatt
     1561 ttgtactgga agcaatcaaa gtaatgccgc aacctactgc acctggtttc ttcactattc
     1621 cagatgatta tacaaaactc acattgcttg caatgccgga taccgcggca ataatggttg
     1681 acacagcaat ggcagaagaa atagaaccgc caccgccccc cgcccctaaa aaagctcctg
     1741 ccaaaacacc cgtaaaagga aaaacttata aatcatccct gcgaaaggaa aattaaagac
     1801 gactattttt cataaaacca caaactcttg cgtttgtggt tttttattgc caatttgtga
     1861 gatcaaaaac cattggacta tgaataagac caccaccctt ctctttctct tcctcatact
     1921 cttttcctgc attaccgcag atgcacagaa aaagggaaaa gataaagaca agggtaagaa
     1981 caagaaagaa
//
LOCUS       DAORLS010000445         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30222_length_1986_cov_1.862248, whole genome shotgun sequence.
ACCESSION   DAORLS010000445 DAORLS010000000
VERSION     DAORLS010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30222_length_1986_cov_1.862248"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PK977_19700"
     CDS             complement(<1..588)
                     /locus_tag="PK977_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF20418.1"
                     /translation="MKYSPVQNYIGGQFVPASSERTLPVISPLDGTQLSTVPLSTAKD
                     LDEAVKAAKAAFPVWSKMPIKERVQVFFKYKYLLEKNLKELAELVQAENGKTYGEAVA
                     EVEKSIELTEFACSLPQLVTGEVLEVSKGVECRTEHVPLGVVASIVPFNFPAMVPNWT
                     IPNAIALGNCMIIKPSEKVPLSTARLAQLLNEAGLP"
     gene            678..1265
                     /locus_tag="PK977_19705"
     CDS             678..1265
                     /locus_tag="PK977_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRF20419.1"
                     /translation="MTPERREKLLMVLSKRQPDLTVVLENVFDPHNISAVMRTCDAVG
                     IQEVFILNTKIPRHKKWGAKSSSSAAKWLTVHQFENTAECFSSLRKRYSRILTTHLSS
                     DAIGLHEINMTEPIALVFGNEHSGVSDEIRALADGNFIIPQVGIIQSLNISVACAVTL
                     YEAFRQKNLAGHYNRDNMDHVMKQELLKEWELGER"
     gene            1284..1775
                     /locus_tag="PK977_19710"
     CDS             1284..1775
                     /locus_tag="PK977_19710"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF20420.1"
                     /translation="MKLGVLLVLWCLLCPGKNSNGQEIKSIKITELERIIAESKTPLM
                     VNFWATWCKPCIEEIPYFIEETNHHRKDSLQLLLVSLDFKEQYPAAILSMAEKRKFTA
                     PIVWLDETNADYFCPKIDPKWSGAIPATLFINNKTGYRNFMEVQLTHEELKKEIMAIL
                     RVH"
     gene            complement(1772..>1986)
                     /locus_tag="PK977_19715"
     CDS             complement(1772..>1986)
                     /locus_tag="PK977_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20421.1"
                     /translation="KNHRLMIPRSLLNKIIIIGFMVLVGFCLAKAIYHQSFMGIVLAL
                     VSLGAGVYFLYLVVKAKAELEAEEAA"
BASE COUNT          582 a          404 c          444 g          556 t
ORIGIN      
        1 cggtaagccg gcttcattta ataattgagc cagtcttgcc gtacttaacg gaactttttc
       61 agagggtttg atgatcatgc aatttcccag ggcaatggca ttagggatag tccagttggg
      121 caccattgcc ggaaaattga aaggaacgat tgatgcgact actcccagcg gtacatgttc
      181 agtacggcat tccacgcctt tgcttacttc cagtacttct cctgttacta attgcggaag
      241 cgaacaggca aattctgtca gctcaatact tttttcaact tcagccacag cttcgccgta
      301 ggtttttccg ttttctgcct gtactagttc agccagttct ttcaggtttt tctccagcag
      361 gtatttatac ttgaagaaaa cctgcaccct ttcttttatt ggcattttgc tccagacagg
      421 aaaggctgct ttggctgctt tcaccgcctc atcaagatct ttggctgtag ataatggcac
      481 agtggataac tgtgtgccgt caagcggaga gatgacaggc aatgttcttt ctgaggaggc
      541 cggtacaaat tgaccgccga tataattctg aacgggggag tacttcatgg tttaggtttg
      601 attatcaggt attcaaattc gccacttata aatatactta atttaacgaa gtttgcaagc
      661 taaagcactc catttcaatg actccggaaa gaagagaaaa gctactaatg gtattgtcca
      721 aacgacagcc tgaccttaca gtagtactcg agaatgtatt tgacccgcac aatatatcgg
      781 ccgtgatgcg tacctgtgat gcagtgggca tacaggaagt atttatcctg aatacaaaaa
      841 ttccccggca taagaaatgg ggtgccaaaa gctcatcaag tgctgccaaa tggcttactg
      901 ttcaccagtt tgaaaataca gctgagtgtt tttcatcact tagaaaacgc tattcaagaa
      961 ttttaacgac acacttaagc agtgatgcta taggtttaca tgaaataaat atgacagagc
     1021 ctatcgcttt ggtatttgga aatgaacata gtggagtaag tgatgaaatc agggctttgg
     1081 ctgacgggaa ttttattatt ccccaggtag gtattattca atcgctgaac atcagtgtgg
     1141 cttgtgccgt tactctttat gaagctttcc ggcaaaagaa ccttgccggt cattacaacc
     1201 gggataatat ggaccacgtc atgaaacagg aattactgaa agagtgggag cttggtgaga
     1261 gataagttta aagtttaatg tttatgaaat taggtgtatt gctggtatta tggtgcttac
     1321 tctgtccggg aaaaaatagt aatggacagg aaataaaaag cataaagatc accgaactgg
     1381 aaaggatcat tgcggaaagc aaaacaccgt taatggtcaa tttctgggcc acctggtgca
     1441 agccctgtat tgaggagatt ccctatttta ttgaagagac aaatcaccat cgtaaagaca
     1501 gcttgcaact tttactggtt agccttgact ttaaggaaca atatccggca gcgatactta
     1561 gtatggcgga gaaaagaaag tttactgcac cgatagtatg gctggatgaa accaatgcgg
     1621 attacttctg tcctaaaatt gaccccaaat ggtccggagc aattccggca acgctgttta
     1681 tcaacaacaa aacaggttac cggaatttca tggaagtgca gctcactcat gaggagctaa
     1741 agaaagaaat catggctata ctccgggtac attaagcggc ttcctctgcc tccagttctg
     1801 ctttggcttt tactaccagg tacaggaagt acactccggc accgagactg accaatgcca
     1861 gtacaatgcc cataaaactt tggtgataaa ttgctttggc caaacaaaag cctaccaaaa
     1921 ccataaagcc gataatgatg attttattca gtagtgatcg tggaatcata agccggtggt
     1981 ttttgg
//
LOCUS       DAORLS010000446         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30390_length_1978_cov_1.028601, whole genome shotgun sequence.
ACCESSION   DAORLS010000446 DAORLS010000000
VERSION     DAORLS010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30390_length_1978_cov_1.028601"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    223..349
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(507..644)
                     /locus_tag="PK977_19720"
     CDS             complement(507..644)
                     /locus_tag="PK977_19720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF20422.1"
                     /translation="MSVGSRYKLYVPYTLGYGAFDYGPIPGGSLLIFEVELLNVIKKQ
                     Q"
     assembly_gap    647..827
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<828..1206)
                     /locus_tag="PK977_19725"
     CDS             complement(<828..1206)
                     /locus_tag="PK977_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRF20423.1"
                     /translation="MKKFVVSAILIGSCSMALAQPKTVPAKQPVPVLKTLSDSASYAI
                     GVSVANFYKQQGINKLNTTLVSKGINDILGNKKALLDDAACNNVMNTYMSKMQAEKSK
                     PRIDSGTNFLAKNKTKPGVKTTAS"
     gene            1300..1737
                     /locus_tag="PK977_19730"
     CDS             1300..1737
                     /locus_tag="PK977_19730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRF20424.1"
                     /translation="MELFSLPVQVRWADLDPNFHLRHSVYYDWGAMCRVEFLNKYGLT
                     AQLMQQLHFGPIIFREECVFRKEVRLGDAVTIDLTLLTAKRDYSRWTIQHHVTKGEGE
                     LSAVLTIDGAWLDTSKRKLAVPPQQAVEVFSQMPAGAAFQWIE"
     gene            complement(1758..>1978)
                     /locus_tag="PK977_19735"
     CDS             complement(1758..>1978)
                     /locus_tag="PK977_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRF20425.1"
                     /translation="TILDKGINGNDQSCILVEEGKFYGMGYIPAGFDTTDYAALKEKL
                     TIYKENSFIRNLVNSYAARFPEKVILLK"
BASE COUNT          431 a          367 c          373 g          499 t
ORIGIN      
        1 aaagtaattg tgctgggtac ccgccagcag atcagggata tgaaaggaaa tttgtcctga
       61 tcaggactcg agaaattttt tgaggtcaac cagtactttg ggccatgctt ctttcacggc
      121 ttcataatac cagtcccagt ctttgccttc ctggtagccg ccttgtacga gatgaacaga
      181 tgttaaaccg ttttcttccc ggaattttat gtcaagatat gcnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccaaagaata
      361 gaggccaccc tgtttttgct ccaacaggca tctttccact ccccaccact tttgcaatga
      421 taaaggattg gtgaacgcat caaagactat tgatgccggc tggtgaatat caatggcagc
      481 ctctactttc cgcatggata aattagttat tgctgtttct ttatcacatt cagcagttct
      541 acttcaaaga tgagtaatga accaccggga atcgggccat agtcaaatgc gccatatccc
      601 aacgtgtaag gaacatatag cttgtacctg gaaccaaccg acatcannnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc tggcagtggt
      841 ctttacaccg ggtttggttt tattttttgc caggaaattc gttcccgaat caatccgggg
      901 ttttgatttt tctgcctgca tcttgctcat gtaagtgttc attacattgt tgcaggcggc
      961 atcatccagc aaagcttttt tattgcccag gatatcattg ataccttttg aaacaagggt
     1021 ggtattcagt ttattaatgc cctgttgttt gtaaaagttg gctacactta caccgatggc
     1081 atagctggcg gagtcgctta atgttttaag aaccggaacc ggctgcttgg ccggaactgt
     1141 tttaggctgg gccaaagcca tactacaact cccgataaga atagcagaaa ctacaaattt
     1201 cttcatatga taattttatt aagtggtaaa aatacaggtt gtaaagctaa ttcgcatggc
     1261 ggtaaagggt taatttcggc ctcaaacctt taattgtcca tggaactgtt ttcactgccc
     1321 gtgcaggtgc gctgggccga ccttgatccg aattttcacc ttcgccattc tgtttattat
     1381 gactggggcg ctatgtgccg ggtggagttt cttaataaat acggtcttac tgcccagttg
     1441 atgcagcaac tgcattttgg ccctatcatc ttccgggaag aatgtgtttt caggaaggaa
     1501 gtgcggctgg gagatgcggt aacgatagat cttactttgc tcaccgccaa aagagattat
     1561 tcccgctgga ccatacagca ccatgtcacc aaaggagaag gtgaactttc tgccgtactt
     1621 acgattgacg gtgcctggct ggacacatca aaaagaaaac tggctgttcc gccccagcag
     1681 gcggttgaag tattcagtca aatgccggca ggggcagctt ttcagtggat tgagtaattc
     1741 ttacaggaga acgaggctta ttttaacaaa attacttttt cgggaaaacg ggctgcatag
     1801 ctattgacca aatttctgat aaagctattc tctttataga ttgtcagttt ttccttcagt
     1861 gcagcatagt cggttgtatc aaagccggcg ggtatatagc ccatgccata aaattttcct
     1921 tcttctacca gtatgcagga ctgatcgttg ccatttatgc ctttatccaa tatcgtaa
//
LOCUS       DAORLS010000447         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30446_length_1974_cov_2.683168, whole genome shotgun sequence.
ACCESSION   DAORLS010000447 DAORLS010000000
VERSION     DAORLS010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30446_length_1974_cov_2.683168"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..618)
                     /locus_tag="PK977_19740"
     CDS             complement(124..618)
                     /locus_tag="PK977_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF20426.1"
                     /translation="MKKLLLFAALFATVTGFAQPGKKSPHDTVSYANGKVTYGRPFKN
                     NRDIFGGLEKYGKVWRLGANEATTISFNSDTKFAGAGVPAGTYTMFAIPNETEWTIIL
                     NSQLGQWGAFGYERNKEKNVAEVTVPVTKLNNVVEQLTIRFDDQNNMIIEWDMTQVKV
                     PLSF"
     gene            complement(682..1179)
                     /locus_tag="PK977_19745"
     CDS             complement(682..1179)
                     /locus_tag="PK977_19745"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF20427.1"
                     /translation="MKVIKLGIISLVVFALMITGISLFFPSRVRISKAIDINAARDSV
                     MRQVTDPVNWKSWYPGTDTSDYLYIEGKVKGFTTGNMRGLMIKTVTDSSVIAGNVGTD
                     AKKGEQGWIIYNSSIPNTVTVQWYMDIHLKWYPWEKFSSLLLEKRYGPTLEKGLENLK
                     AQLEK"
     gene            complement(1193..>1974)
                     /locus_tag="PK977_19750"
     CDS             complement(1193..>1974)
                     /locus_tag="PK977_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF20428.1"
                     /translation="SDNPHTTVQVRSTTASANTLGSFVPVLDYLKLRIETALAEPREE
                     LIKIEEPEVKEVRKYRAIDFILPGQLGFSILFSTLFGVAFTFFGMREQLVLKRFYASP
                     VNRINILLGIGASRLFFQLLSVVVLILFGYFAMNFTLVNGIFTFLEIILMTIIMLFLL
                     MGVGLIFSSIAKTDNSIPLLINLFGFPQMMLSGTFFPIEVFPKWLQEICRVLPLTQFN
                     DSIRKISFEGQSIVECWKEIGILGIWMIVIYAIAVKVLKWE"
BASE COUNT          580 a          445 c          372 g          577 t
ORIGIN      
        1 tgaaagaaaa gaatggtagg atctgtttcc ggcactatag cagtatcggc cggttcttgt
       61 gcaaatgcgg ttaaccggga tgctacaaaa agcagcgata agaatttttt cataaatgag
      121 caatcaaaaa cttaagggaa ctttcacctg tgtcatatcc cactcgatga tcatattatt
      181 ttgatcatca aatcggatgg ttagttgctc taccacgtta ttgagttttg taacgggcac
      241 cgttacctcg gctacattct tttccttatt tcgttcataa ccaaatgcgc cccattggcc
      301 cagctggctg ttgagaatga tcgtccactc tgtttcattg ggaatagcaa acatggtata
      361 ggtacctgcg ggtacacctg cccctgcaaa cttggtatca ctattaaaac taatggtagt
      421 cgcttcattt gcgcccaacc gccatacttt gccatatttt tcgaggccac caaaaatatc
      481 cctgttgttt ttaaaaggtc tgccataagt tacttttccg ttcgcataac ttacggtatc
      541 atgcggactc ttcttaccgg gctgtgcaaa tcctgtaaca gtggcaaaaa gagctgcaaa
      601 caataataac ttcttcatag ctgaatgtat taaatgaggt gattcattaa agttagcaag
      661 attaataaaa tagtaacccg gttatttttc caactgtgct ttcaggtttt ccaggccttt
      721 ttcaagcgtt ggcccatatc gtttttccag caggaggctg gaaaattttt cccaaggata
      781 ccatttcagg tgaatatcca tataccactg tacggtaaca gtgttgggga ttgaagaatt
      841 gtaaatgatc cagccctgct cccctttttt ggcatctgta ccaacatttc cggctatgac
      901 tgacgaatcg gttaccgttt taatcatgag tccccgcata ttccctgtag taaatccctt
      961 cacttttcct tcaatataaa ggtagtcaga tgtgtctgtg ccgggatacc agcttttcca
     1021 gttgaccgga tcggtcacct gtctcatcac tgaatccctg gctgcattaa tatctatcgc
     1081 ttttgatatc ctgaccctgg aaggaaagaa cagggagata cctgtgatca tcaaagcaaa
     1141 caccacaaga cttattattc ccagctttat gaccttcata ctaataactt ttttattccc
     1201 acttcagtac tttcactgca atggcatata tcactatcat ccagataccc agaatgccga
     1261 tctctttcca gcattcaacg atactttgac cttcaaacga aatcttgcgg atggaatcat
     1321 tgaattgcgt caatggcaaa acccggcata tctcctgcag ccatttaggg aatacttcta
     1381 tggggaaaaa agttcctgac agcatcattt gtggaaagcc aaacaggtta atcaacagcg
     1441 gaattgaatt atctgttttg gcaatactgc taaagatcag ccccaccccc atcagcaaaa
     1501 acaacatgat gatggtcatt aatatgatct ctaaaaaagt aaatatgcca tttaccaggg
     1561 tgaagttcat ggcaaaatag ccaaacaaga taagtaccac cacactcaac aactggaaga
     1621 ataaacggct ggcaccgatg cccagcaaaa tattgatacg atttaccggt gaagcataaa
     1681 atcgcttaag tacaagttgt tccctcatac caaaaaatgt aaatgctacc ccaaataagg
     1741 tagagaacaa tatggaaaaa cctaactgcc caggaagtat aaaatctatt gcacggtatt
     1801 ttcttacttc ttttacttca ggctcttcaa tttttataag ttcctctctg ggttcagcca
     1861 aagctgtttc aatccgtagt ttcaggtaat ctaaaaccgg cacaaaggaa ccaagtgtgt
     1921 tggcactggc tgttgtggac ctgacttgca cagtagtatg agggttatcc gatg
//
LOCUS       DAORLS010000448         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30515_length_1971_cov_1.479645, whole genome shotgun sequence.
ACCESSION   DAORLS010000448 DAORLS010000000
VERSION     DAORLS010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30515_length_1971_cov_1.479645"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>282
                     /locus_tag="PK977_19755"
     CDS             <1..>282
                     /locus_tag="PK977_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF20429.1"
                     /translation="KMLVDQLREEDKISMVVYAGAAGLVLEPTSGDEKTKIKDAIDKL
                     EAGGSTAGGAGIKLAYKTARENFVKGGNNRVILCTDGDFNVGESSDDAM"
     assembly_gap    283..377
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <378..>655
                     /locus_tag="PK977_19760"
     CDS             <378..>655
                     /locus_tag="PK977_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HRF20430.1"
                     /translation="KGNGNHAYIDGISEAKKVLVNEFGGTLFTIAKDVKLQVEFNPAK
                     VQGYRLIGYENRMLAKEDFNDDKKDAGELGSGHTVTALYEVIPVGVKS"
     assembly_gap    656..743
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <744..1081
                     /locus_tag="PK977_19765"
     CDS             <744..1081
                     /locus_tag="PK977_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HRF20431.1"
                     /translation="LTVKFRYKAPDGDVSKLIEHPVLDKQVALTKTSDNFRFAAAVAQ
                     FGLLLRESEYKSTASYDNVLNLARKAKGYDEEGYRSEFVRLVESARLLAKGKPEPKSG
                     EEPSEEDSK"
     gene            1251..>1713
                     /locus_tag="PK977_19770"
     CDS             1251..>1713
                     /locus_tag="PK977_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF20432.1"
                     /translation="MRIYFTLGICMFLNTAFPQNNKVQEPEVVTRCTLRPVIEFPVTF
                     TIKDQNDGSPVPRATVSVFRPDKNDSVHLVANDSGSVWVKLLGRTDKYVVSISTVGYV
                     DTSFLVDRTGKYEIILNKHYMEIKPVILSSLPMICCLCYKKLLCYRVKHEKR"
     assembly_gap    1714..1734
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1744..>1971
                     /locus_tag="PK977_19775"
     CDS             1744..>1971
                     /locus_tag="PK977_19775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20433.1"
                     /translation="MDIQNDNFKIYPNPSLRGSSAVIEFESLNQENSQVRIFSINGQL
                     VKALPVYAVKGKNKLQVNIDSRWPAGTYIFQL"
BASE COUNT          559 a          308 c          426 g          474 t
ORIGIN      
        1 gaaaatgctg gtggatcagt taagagaaga ggataaaata tcaatggtag tttatgcggg
       61 tgcagctggt ttggtattgg agccaaccag cggcgatgaa aaaacaaaaa ttaaggatgc
      121 tattgataaa ctggaggccg gcggctcaac agccggcggg gcaggtatta aactggctta
      181 taaaacagca agggagaatt ttgtgaaggg tggtaataac cgggtgatac tttgcacgga
      241 tggtgatttt aatgtgggcg aaagcagtga cgatgccatg gannnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnntaa gggcaacgga aaccatgcct atatagatgg tatcagcgag
      421 gcaaaaaaag tattggtaaa tgaatttgga ggcactctgt ttacgattgc caaagatgta
      481 aagctccagg tggaattcaa tcctgccaaa gtacaaggtt accggctgat cggttatgaa
      541 aaccgtatgc tggccaaaga agattttaat gatgataaaa aggatgccgg tgaactgggc
      601 agtggtcata ctgtaaccgc cctatacgag gtaataccgg ttggggtaaa gagtgnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnttttgac tgtgaagttc cgatacaaag ctcctgatgg
      781 tgatgtaagc aagctaattg aacatcctgt cctggacaag caggtggcat tgaccaaaac
      841 atccgacaat ttccgatttg cggctgctgt ggcccagttt ggcctgcttc tccgtgaatc
      901 agaatataaa tcaacagctt catatgataa tgtgctgaac ctggcccgta aggcaaaagg
      961 atatgatgaa gaagggtatc gttcggagtt tgtaagactg gtggaaagtg cccggttact
     1021 ggctaaaggc aaaccagaac ctaaaagcgg ggaagagcct tcagaagaag attctaaata
     1081 aaaaaaagtt aaagcagcat atgtaaacag cgccattgcg tcatcctgtt gaaaatagct
     1141 aatcatttaa cagccggtta gtgataacgg ggctggtttt taccggcccc ttttttgtct
     1201 gaaccaggat ttataagatt aaatgatttt agggatttaa aattttgctt atgagaatat
     1261 attttacact tggtatttgc atgttcctaa atactgcttt cccccaaaac aataaagtgc
     1321 aggaaccgga agttgtaacc cgctgtaccc ttcggccagt aattgaattt ccagttacgt
     1381 ttacaataaa agatcaaaac gatggcagcc ctgttcccag agctacggtc tcagttttcc
     1441 gcccggataa aaatgattcc gttcatctgg tggccaatga tagcggatcc gtatgggtaa
     1501 aacttttggg tagaacggat aaatatgtcg taagtataag tacagttgga tatgttgaca
     1561 cttcattttt agttgatagg acaggtaaat atgaaattat tcttaacaaa cactatatgg
     1621 aaataaagcc tgtcattctc tcctcattac caatgatatg ctgcctttgt tataagaaac
     1681 tgctctgtta ccgggtaaag catgaaaaga gggnnnnnnn nnnnnnnnnn nnnngtttta
     1741 acaatggata ttcaaaatga taattttaaa atctatccca atccatcttt aagaggtagt
     1801 tcggcagtga tagaatttga gtcgttgaat caggaaaact cacaagtaag aatattttcc
     1861 atcaacggcc aattagttaa ggctttgcca gtatatgcag tgaaaggaaa aaacaagctg
     1921 caggttaaca ttgacagccg ctggccggcg ggtacatata tttttcagct t
//
LOCUS       DAORLS010000449         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_30889_length_1949_cov_1.832101, whole genome shotgun sequence.
ACCESSION   DAORLS010000449 DAORLS010000000
VERSION     DAORLS010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30889_length_1949_cov_1.832101"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..>994
                     /locus_tag="PK977_19780"
     CDS             93..>994
                     /locus_tag="PK977_19780"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF20434.1"
                     /translation="MKKIFLMASALSYLSAHAQDIPARIDEYFKKQHDEGAFNGNVLI
                     ASHDTIIYKQSFGLAEIATGRKLNDTSVFELASVSKQFTAMGIVLLERDSKLKYTDSL
                     RHFFPDMPYSNITIEHLLHHTSGLPDYMDLVKNKTERGSIIGNEDLIRLLIQFKPPVN
                     FKPGEKWEYSNTGYALLAGIIEKVSGQPFGEYLKAKIFKPLGMRHTEVYRRRLENRKV
                     ENYAYGYVKESPDSKNMILPDDHPNYKDMVHRLDGIVGDGTVNSTTGDLLTWSLALDN
                     NRLVPAGVKERIFSAGKLNNGEMH"
     assembly_gap    995..1111
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1112..1485
                     /locus_tag="PK977_19785"
     CDS             <1112..1485
                     /locus_tag="PK977_19785"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HRF20435.1"
                     /translation="RKTIILLNNYDNMLIQYDKLLNIIDGIKEEARKEIKLDSLKLKQ
                     YEGIYQLMPEFFITITAKGERIFAQVTGQGQFEIYPEKEDLFFYKVVEAQLKFIRNEQ
                     KEIISTILIQNRREVDCKKIK"
     gene            1505..>1949
                     /locus_tag="PK977_19790"
     CDS             1505..>1949
                     /locus_tag="PK977_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF20436.1"
                     /translation="MHRFKLLILLVTITVSFCRAQYPFNNPSLPVDKRVHDLLDRLTT
                     EEKISLLLYNSPGVERLGIPAYNWWNEALHGVGRAGRATVFPQAIGMAATFNEALIKE
                     VGTVISNEARAKYNIAVKNNNRGQYLGLTFWSPNINIFRDPRWGRG"
BASE COUNT          588 a          331 c          428 g          485 t
ORIGIN      
        1 gcgaagattt atggttacaa taaaatctgg atcgacaaag aagatgatcc gtatgcgtat
       61 ggcttccagg tgatataatt aattgatgtg atatgaaaaa gattttctta atggcatccg
      121 ctttatcata tttatctgca catgcacagg acattcctgc ccgaattgac gagtacttca
      181 aaaagcaaca tgatgaagga gcctttaatg gcaatgtgct gattgcttcg catgatacta
      241 ttatatataa gcagagcttt ggcctggcag aaatagctac tggccgaaag ctgaatgata
      301 cttctgtttt tgaactggca tctgtttcca aacaatttac agcaatggga attgtattgt
      361 tggaaagaga tagcaaactg aaatataccg acagcctgcg gcattttttt cccgacatgc
      421 cttacagtaa cattaccatt gaacatcttt tgcaccatac ttcaggattg ccggattata
      481 tggatttggt aaaaaacaag acagaaagag gttcgattat cggcaatgaa gatctgatca
      541 ggttgttgat acagttcaaa ccaccagtta attttaagcc cggcgaaaaa tgggaataca
      601 gtaataccgg ttatgctttg cttgccggta ttatcgaaaa agtttcgggg caaccattcg
      661 gtgaatattt gaaagcgaaa atatttaaac cattgggtat gcggcatact gaagtgtaca
      721 ggcgcagact ggaaaaccgc aaagtagaaa actatgccta tggctatgtt aaagagtcgc
      781 cggactcaaa gaatatgatt ttgccagacg atcatcctaa ctataaagac atggtgcacc
      841 gactggatgg gattgtgggt gacggtaccg ttaattccac aacgggagat ttgctgacat
      901 ggtcgctggc actggataac aatcgcctgg ttcctgccgg ggtgaaggaa agaatttttt
      961 cagccggaaa gcttaacaat ggagaaatgc acagnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccaggaaga ctattatcct gctcaacaat
     1141 tatgataata tgctaattca atatgataaa ctgctcaata taattgatgg gataaaagag
     1201 gaagcaagaa aagaaattaa acttgattct ctgaagttaa agcaatatga aggaatttac
     1261 cagctgatgc ccgagttttt tatcaccatt acagccaaag gtgaaaggat atttgcacag
     1321 gttacagggc agggacagtt tgagatatac cctgaaaagg aggacctgtt cttttataaa
     1381 gtagtggaag cgcaactgaa gtttattaga aatgaacaaa aggaaatcat ctcaacgata
     1441 ctgatacaga accggaggga ggtggattgt aaaaaaataa agtgacccaa taaacagaac
     1501 atccatgcac cggttcaagc tgcttatttt attagtaaca attacagtat ccttttgccg
     1561 ggcacagtat ccttttaaca atccttcatt acccgttgat aaaagagtac atgatttact
     1621 tgaccggctg acaacggagg agaagatcag cctgcttttg tataatagcc caggagtaga
     1681 gcggttgggt attccggctt acaactggtg gaatgaggcc ttgcatggag ttggcagggc
     1741 aggcagggca acggtatttc cgcaggcaat cggtatggcc gctactttta atgaggcgtt
     1801 gataaaagag gtgggaactg ttataagtaa tgaagcaagg gccaagtata atattgcggt
     1861 taaaaataat aaccgggggc aatacctcgg actgacgttt tggtcaccta atatcaacat
     1921 cttccgtgat ccccgatggg gccgtggcc
//
LOCUS       DAORLS010000450         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_31115_length_1937_cov_1.934644, whole genome shotgun sequence.
ACCESSION   DAORLS010000450 DAORLS010000000
VERSION     DAORLS010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31115_length_1937_cov_1.934644"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PK977_19795"
     CDS             complement(<1..699)
                     /locus_tag="PK977_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRF20437.1"
                     /translation="MKDFSYITNSSPAYIESLYREFLNNPGAIDPEMKKFFEGFDFAI
                     SHAPINGKPTGVIEAKSTDGKPGDIVSIDWMKEVMAYRLILGYRNKAHLIANTNPIRK
                     RKDRGANIDLDFYGFTESDLDKEFYAGQFLGLGKAALRTILSHLEKCYASSVGVEFKY
                     ISDQKKIDWLTNEIEKTMLEPLPLVKKRRALEKLNQGVMFEKFLHTKYVGQKRFSLEG
                     GETTIAALDAIINTA"
     gene            931..1182
                     /gene="rpsT"
                     /locus_tag="PK977_19800"
     CDS             931..1182
                     /gene="rpsT"
                     /locus_tag="PK977_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRF20438.1"
                     /translation="MANHKATKKDARQAAKRRDRNRYYGKTTRNAIRDLKAADDKQMG
                     EKFPEVAGMIDKLAKRGIIHKNKAANLKSKLAKKVNAAK"
     gene            1257..>1937
                     /locus_tag="PK977_19805"
     CDS             1257..>1937
                     /locus_tag="PK977_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRF20439.1"
                     /translation="MRHILVILFSIQCLCSASQSSIATKFELSKGTQTPTYFEIVEWW
                     KKLDGISGKVKLLTMGMTDAGYPLHLSVVSNNGDYNFDNIRKNNKRIILVNNGIHPGE
                     PDGIDASMLLARDIVTGKYKIADNVVLAFIPVYNIGGCLNRSANFRVDQNGPEEFGFR
                     GNSQNLDLNRDMIKCDSKDAKAFTEIFHLLDPDVFIDNHVSNGADYQHVMTLLTTQNT
                     KLGGVMGEY"
BASE COUNT          605 a          368 c          389 g          575 t
ORIGIN      
        1 tgcagtattg ataatagcat caagggcagc aatagtggtt tcgccgcctt ccaatgaaaa
       61 tcttttttgc cctacatact tggtgtgaag aaatttttca aacatcacac cctggttaag
      121 tttctccaaa gcccttctct ttttaacgag aggcaggggc tcaagcattg tcttttcaat
      181 ctcattggtg agccagtcta tttttttctg gtcacttata tatttgaact caacaccaac
      241 actggaagca taacatttct ccaggtgtga caagatagtg cgtaatgctg ctttacctaa
      301 accaagaaat tgacctgcat aaaactcttt gtcaaggtca gattcagtaa atccgtaaaa
      361 gtccaggtca atattagctc cccgatcctt tctcttcctg atagggtttg tattggctat
      421 caggtgggcc ttattgcggt atcctaaaat caagcgataa gccatgactt ccttcatcca
      481 gtcgatacta acaatgtctc cgggttttcc atcagtactt tttgcctcaa taactccagt
      541 tggttttccg tttattggtg catggctaat agcaaaatca aacccttcaa aaaacttctt
      601 catctcaggg tcaattgctc cgggattatt cagaaactct ctgtacaggc tttctatata
      661 agcaggcgat gaatttgtga tgtaggaaaa atccttcatg agggtgaaag tttggaagta
      721 accaatttta gaggcacaaa tatcgcccaa aaatgggaaa accagccctc attttgagcg
      781 ttttaagtac ttaattaact ggctggcaat ggaatattgg cagcattttc agggcttcaa
      841 aatatagttt tgaataccca attaaatagc tgaaaatttg gtggttaaac tgcccgggct
      901 tacctttgcc gtccctaaaa agtaataaga atggctaatc ataaagctac taagaaggat
      961 gcccgccagg cggcaaaacg tcgtgacaga aacaggtatt acggaaaaac tacccgtaat
     1021 gcaatccgtg atctcaaggc agctgatgac aaacaaatgg gggaaaaatt ccctgaagtg
     1081 gccggaatga tcgataaact ggctaaacgt ggcattattc ataagaataa agctgctaac
     1141 ctgaaaagta agctggctaa gaaagttaac gcagcgaagt aatcactact aataaattat
     1201 gcaaaaggca ttcctcattg gaatgccttt tttattttta gaacttaatg atttatatga
     1261 gacatatcct tgtcatactt ttttctattc aatgcctttg ttctgcctct caaagcagta
     1321 tcgctacaaa atttgaatta tcaaaaggaa cgcaaacacc tacatatttt gagatagttg
     1381 agtggtggaa aaaacttgac ggaatatcag gtaaagtaaa attattaaca atgggcatga
     1441 ctgatgctgg ctatccgctt catttgtctg ttgttagcaa taatggagat tacaacttcg
     1501 acaatatcag aaaaaataat aaacgaatta ttcttgttaa caatggcatt catcccggcg
     1561 aaccagatgg cattgatgcc agtatgctgc tggcaaggga cattgtaaca ggcaaataca
     1621 aaattgccga caatgtggta ttggccttta tcccggttta taatattggc ggttgtctta
     1681 acagaagtgc caattttcgt gttgatcaga atgggccgga agaatttggt tttcggggaa
     1741 attcacaaaa tcttgatctg aatcgggata tgattaaatg tgattctaaa gatgccaaag
     1801 ctttcacaga aatatttcat ttgcttgatc cggatgtatt tatagataat catgtgagca
     1861 atggggcaga ttatcagcat gtgatgacat tacttaccac tcaaaacacc aagttgggag
     1921 gtgtaatggg ggaatat
//
LOCUS       DAORLS010000451         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_31242_length_1930_cov_1.622400, whole genome shotgun sequence.
ACCESSION   DAORLS010000451 DAORLS010000000
VERSION     DAORLS010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31242_length_1930_cov_1.622400"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..622
                     /locus_tag="PK977_19810"
     CDS             83..622
                     /locus_tag="PK977_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HRF20440.1"
                     /translation="MPSVATDPRYPIGKYEPQPFSIEQKVEWLADLKFLPLLLENAIL
                     NLDEAQLHTPYREGGWTVHQLIHHVADSHINAYCRFKLALTEDNPTVKPYDENKWAEL
                     YDVQKLPVNYSITLLHAIHARLFETLKYVSDNDWNNRTIFHPEHKKTMRLWYLLGMYA
                     WHGKHHVAHITSLRERMKW"
     gene            630..1178
                     /locus_tag="PK977_19815"
     CDS             630..1178
                     /locus_tag="PK977_19815"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF20441.1"
                     /translation="MKWKTLSSENLFNDKWFKVRKEVCETPLGKIVDPYYVYDFSTWV
                     GALPVTEEGKIIMLKQYRHALGEVCYEIPGGCVDATDKSFEEAIARELLEETGYSFSS
                     FEYMGKISPNPSTNSNLLHMFLAKGGRKVAAQSLDENEEIEVMLLEMDELKQLLRENK
                     IVQSMHVSCIMYALERMGELKY"
     gene            complement(1175..>1442)
                     /locus_tag="PK977_19820"
     CDS             complement(1175..>1442)
                     /locus_tag="PK977_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20442.1"
                     /translation="KAQIPRALQEEELQKKAAIHQLKGNFYSNVNTALYAASLNAKKE
                     DMIVVCGSIFVVGEISLKSIEAIWGKEDYTGDSLHFLDTLEFFS"
     assembly_gap    1443..1559
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1560..>1930)
                     /locus_tag="PK977_19825"
     CDS             complement(<1560..>1930)
                     /locus_tag="PK977_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HRF20443.1"
                     /translation="VFEMAAKEKNTALHIAEQNWQCLDSKWENLKLLVTVLNEQTETY
                     QLDLPGIYQTRNLLTVLESSRVLEKEGWKINSDHFKTALSQVKKLTGLHGRWEIVRHS
                     PLLVLDVAHNANGIEQLTEQI"
BASE COUNT          542 a          330 c          381 g          560 t
ORIGIN      
        1 caggggagta gggaatgata taccaggcgg agctacgcta atatttgaag tagaactttt
       61 acaagtaatt aaatgatcat ttatgccatc tgttgcaact gacccaagat atcctattgg
      121 taagtatgag ccacagcctt tttctattga gcaaaaagta gaatggctgg ccgatttaaa
      181 atttttgccc cttcttttag agaatgcaat tctcaattta gatgaggcac aacttcacac
      241 tccttaccgg gaaggtggct ggacggttca tcagcttatc catcatgtgg cagatagtca
      301 tatcaatgcc tattgccgtt tcaaactggc tttgacggaa gataatccca ctgtcaaacc
      361 ctatgatgaa aataagtggg cagaattata tgatgtacaa aaattacctg taaattattc
      421 cataaccctg ttacatgcta tacatgcaag gctttttgaa acacttaagt atgtaagtga
      481 taatgactgg aataaccgga ccatttttca ccccgaacat aaaaagacaa tgcggttatg
      541 gtacttgtta ggcatgtatg catggcatgg caaacaccat gtagcgcata taacttcact
      601 aagggaaaga atgaaatggt aagccgatca tgaaatggaa gacactatct tctgaaaacc
      661 tgtttaacga caaatggttt aaggtaagaa aagaggtttg tgagacacct ctgggaaaaa
      721 ttgtagatcc ctactatgta tatgattttt ctacctgggt aggcgccttg ccggttactg
      781 aggaggggaa aataataatg ctcaaacaat accgccatgc actgggtgag gtttgttatg
      841 aaattcccgg tggttgcgtg gatgctactg acaaaagctt tgaagaagca atagcaaggg
      901 agttgctgga agaaacaggg tatagtttct cttcttttga atacatgggt aagatatcgc
      961 ctaatccttc tactaattcc aaccttttac atatgttcct ggcaaaagga ggcagaaaag
     1021 tggctgcaca atctttggat gaaaacgaag aaatagaagt gatgctgctg gaaatggatg
     1081 agttaaagca attattgaga gaaaacaaga ttgttcaatc gatgcatgta agttgtataa
     1141 tgtatgcact ggagcggatg ggagaattaa aatattagct aaagaattca agggtgtcca
     1201 aaaagtgcag gctatctcct gtataatctt ctttacccca aatagcctca atgcttttca
     1261 ggctgatctc cccgactaca aaaatgctgc cgcaaacaac tatcatatct tctttttttg
     1321 cattgagtga agcagcgtaa agtgctgtat taacattgga atagaaattt ccttttaact
     1381 ggtgtattgc tgcttttttt tgtaactctt cttcctgtaa agctcttgga atttgtgcct
     1441 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1561 atttgttcgg tgagttgctc gataccgttt gcgttatgtg ctacatccaa taccagcaaa
     1621 ggggaatgcc tgacaatctc ccatcttccg tgcaggcctg ttaatttttt tacctgggag
     1681 agggcggtct taaaatgatc tgaatttatt ttccatcctt ctttttctaa cacacggctt
     1741 gattctaaaa cagtaagcag gtttcttgtt tggtaaatgc ccggcaagtc caattgataa
     1801 gtttcagttt gttcatttaa aacagttact aaaagcttta gattttccca ttttgaatca
     1861 aggcattgcc agttttgttc tgctatatga agggcggtat ttttttcttt tgctgccatt
     1921 tcaaataccg
//
LOCUS       DAORLS010000452         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_31614_length_1911_cov_1.880388, whole genome shotgun sequence.
ACCESSION   DAORLS010000452 DAORLS010000000
VERSION     DAORLS010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31614_length_1911_cov_1.880388"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>312)
                     /locus_tag="PK977_19830"
     CDS             complement(<1..>312)
                     /locus_tag="PK977_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF20444.1"
                     /translation="SDSRKTAFVEPEETIELNNEVHELENAERKEVYRILRELTAKLS
                     VYASLLTNYHAVIGEYDFIRAKAKLGIEIKGEYPLLTDKSHIHLVEAYHPLLYLYNQK
                     AG"
     assembly_gap    313..386
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<387..673)
                     /locus_tag="PK977_19835"
     CDS             complement(<387..673)
                     /locus_tag="PK977_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF20445.1"
                     /translation="MEKIFRWFDSERRIAYQGLAKVIDGTYYEKMIIELIDDVLDDYG
                     QVKDNASDELKNIRLSLYRKRNELRRLFEKIVSKLNKAGYLADIEESFMNG"
     assembly_gap    757..787
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<788..993)
                     /locus_tag="PK977_19840"
     CDS             complement(<788..993)
                     /locus_tag="PK977_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20446.1"
                     /translation="MKLYPESASVQLEFNKIKDLLVNYCQAEHARDKAKQLRIHTRQE
                     FIELELKQSHEYRQLIVNGIYFPN"
     gene            complement(1049..1486)
                     /locus_tag="PK977_19845"
     CDS             complement(1049..1486)
                     /locus_tag="PK977_19845"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRF20447.1"
                     /translation="MFTSCNNNKVDKSENTIVAFRLKPGEDLKGGIEKVVKENNISAG
                     WVATAVGSLTDFAIRFANQPEAAIGSGHFEIISLVGTVSVNGSHLHISISDSTGQTIG
                     GHLVKGCKVYTTAEIVIQATSKYEFKREKDGTTPWEELQIKEK"
     gene            1604..>1911
                     /locus_tag="PK977_19850"
     CDS             1604..>1911
                     /locus_tag="PK977_19850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HRF20448.1"
                     /translation="MKIVFPSLLISLTSLISCAQNNPVENKTNATNKNNSMTSENTIT
                     GVTDTATFANGCFWCTEAIFEELKGVISATSGYTGGQTENPTYKEVCTGETGHAECLQ
                     "
BASE COUNT          497 a          419 c          312 g          578 t
ORIGIN      
        1 tcctgctttt tgattgtaca aatataacag ggggtgatat gcttctacaa ggtgaatgtg
       61 tgacttatct gtcagtaacg ggtattctcc ctttatctct attcccagtt ttgcctttgc
      121 cctgataaaa tcatactcac ctataacagc atgatagttg gtaagcaggg atgcataaac
      181 agaaagtttg gccgtcagct ctctcaaaat cctatacact tctttacgtt ctgcattctc
      241 cagttcatgt acctcgttat tcagctcgat agtttcttct ggctctacaa aggctgtttt
      301 ccggctgtcg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnncttc cgttcatgaa actttcctca atgtcagcaa
      421 ggtaaccggc cttgttaagc tttgaaacaa ttttttcaaa caagcggcgc aattcatttc
      481 ttttccggta cagactaagg cggatatttt ttagttcatc tgaagcattg tctttcacct
      541 gtccataatc atccagcaca tcatctatga gttcaatgat cattttctcg taataagtgc
      601 cgtcaataac tttagctaag ccctgatatg caatccttct ttcactatca aaccaccgga
      661 atatcttttc catactttct gccagtttcc ttagctgcat gaattgttca ccggttaaaa
      721 cagctccagg aatggaaagc aatttcaatt cctttgnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnntca ttcgggaaat aaataccgtt gacgatcaat tgcctgtact catggctttg
      841 ctttaactcc agctctataa actcctgtcg ggtatgtata cgcaactgct ttgctttgtc
      901 acgggcatgc tctgcctggc agtaattgac aagcaaatcc tttatcttat taaattccag
      961 ttgaacggag gcagattcag ggtataattt cattcgctgc tacaggctat tttcggtcgg
     1021 cgaaattaca accggggagg tcattaatct atttttcctt aatctgcagt tcttcccaag
     1081 gcgtagtgcc atctttttct cttttaaatt catatttgga ggtagcctgg atgacaattt
     1141 cggccgttgt atacacttta catcccttta caagatgccc gccaatagtt tggcccgtgc
     1201 tgtcgctgat acttatatga agatgtgagc cattcacact gactgtacca acaaggctta
     1261 ttatttcaaa atgcccgctg ccaatggcag cttccggctg gtttgcaaac ctgatggcaa
     1321 aatcagtaag actccccaca gctgttgcaa cccatcctgc tgaaatattg ttttccttca
     1381 ccactttttc aatccctcct ttcaggtcct cacccggttt caaccggaaa gcaacaattg
     1441 tattttcgct tttatccact ttattattat tacaagatgt aaacaaaata attgttaaac
     1501 aaaccagcaa aaaatttgct acccatccat atctttcaac cattcgtaga tgaatttgtt
     1561 agttttgaaa caaatattga ctatatatct aaacctgtcg aaaatgaaaa ttgttttccc
     1621 ttcgctcctt atatcactga cttcattgat atcctgtgca caaaataacc cggttgaaaa
     1681 taaaaccaat gctacaaata aaaacaatag tatgaccagc gaaaatacca taacaggagt
     1741 aaccgatacc gccacatttg ccaatggctg tttttggtgt accgaagcaa tttttgagga
     1801 attgaaagga gtaatcagcg ccacttctgg ttataccggc ggccaaaccg agaatcctac
     1861 ttataaagaa gtatgtaccg gcgaaaccgg tcatgccgaa tgtttgcaaa t
//
LOCUS       DAORLS010000453         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_31668_length_1909_cov_1.398058, whole genome shotgun sequence.
ACCESSION   DAORLS010000453 DAORLS010000000
VERSION     DAORLS010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31668_length_1909_cov_1.398058"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..>823
                     /locus_tag="PK977_19855"
     CDS             467..>823
                     /locus_tag="PK977_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20449.1"
                     /translation="MKAALFPLFDQGVNLPDTKNDIPDILLSNVFNDNTGSYLNNFNL
                     YLAYYRKIPIVITEIKINCKSANKWFLTNFHSQIIDNYYNRIYFSRTKKQQVDDVFYF
                     LYDDLMVHFDTNHSKVT"
     assembly_gap    824..852
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            875..>1201
                     /locus_tag="PK977_19860"
     CDS             875..>1201
                     /locus_tag="PK977_19860"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF20450.1"
                     /translation="MLELSSHLKKFKVRSPRSLPQISVLVNTIRGIDLKSLEIHKARL
                     RIQDNYNDDFIKIHDTIYNRLSKRNDKGIVLLHGRPGTGKTSYLRYLIATVKKNIILL
                     PPDMAAA"
     assembly_gap    1202..1227
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1228..1603
                     /locus_tag="PK977_19865"
     CDS             <1228..1603
                     /locus_tag="PK977_19865"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF20451.1"
                     /translation="NKNSILVIEDAENIVVSRDKNSKSPVSALLNITDGLLSDCLNIQ
                     VICSFNTDISKIDGALLRKGRLIAKYEFRELSAAKANALSVKLGFNKEFTQPASLADI
                     YNQSEDFCVQDNQSQPLGFKLS"
     gene            1638..>1909
                     /locus_tag="PK977_19870"
     CDS             1638..>1909
                     /locus_tag="PK977_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20452.1"
                     /translation="MEKLLVQNTDIATEEVIRRLTQEQIEQFTIVHCRIFSIYPTKMR
                     IWPSTFLVEEEGNRRKLLHNFNISLYPGWTDYFVKNDYIRFTLVFEG"
BASE COUNT          606 a          297 c          345 g          606 t
ORIGIN      
        1 aagatctcaa agttcgagag ggccgaaaat ccaatgactg taagtatacc tggtaaagat
       61 cttccatttc ctgatttagc tcaaagggct taaatacagt tgtaaatttt ttgtttgttc
      121 ttattaatct ttgactttta tttcgggaga agatattttc aagtctgtac ctgagccaaa
      181 agactctgaa aaaatcatta tccggaaaaa tgaaatcagt ggtaaaaatt gactggccca
      241 tcctgaacca gcctctttcc aggtaggcat caagcgtatt tccagttact ctatcagggt
      301 attcaatttc attagtcatt gctattgcct tctggtgcta atttattacg cataattgta
      361 acctatttgc aggatgaagt taatatttca ttgcaaatag gttacggtgc cggggtcata
      421 taattgcagc gtaatccaga ttaatctaaa aataaatgta taaataatga aagcagcatt
      481 atttcccctt tttgaccagg gggtaaactt gccggacact aaaaatgaca ttccggatat
      541 tttgttatca aatgtcttta acgataatac tggctcatac ctgaataatt ttaatcttta
      601 cttagcctat tatagaaaaa tacctattgt tattacagaa attaaaataa actgcaaatc
      661 ggctaataag tggtttttga cgaattttca cagtcagatt attgataatt attataatag
      721 gatttatttt tcaagaacaa aaaagcaaca ggtagatgat gttttctatt ttctttatga
      781 tgatctaatg gtgcattttg atacaaatca ttcaaaagtg accnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nncaaaaaaa ctgatgaaaa actggtgcta gaattatcat cacatcttaa
      901 aaaatttaaa gttcgaagtc ccaggagctt acctcaaata tcagtactgg taaacacaat
      961 aaggggtatt gatttgaaga gtttggaaat acataaggcc cggttaagaa tacaagataa
     1021 ctataacgat gattttatta aaatacatga taccatatat aatcgtcttt caaaaaggaa
     1081 tgataaggga attgttttgt tacatgggag gcccggaacc ggtaaaactt cctatctgcg
     1141 atacctgatc gctacggtga agaaaaacat tattttatta ccgcctgata tggccgccgc
     1201 tnnnnnnnnn nnnnnnnnnn nnnnnnntaa taaaaactct atactggtaa ttgaggatgc
     1261 tgagaatatt gttgttagtc gtgataaaaa ttcaaagtcg ccggtttctg ccttgttgaa
     1321 tataactgat gggcttttat cggattgcct gaatatccag gtaatctgtt cttttaacac
     1381 cgatatatca aaaattgacg gagccttgtt acgaaagggg agattgatag caaaatatga
     1441 gttcagggag ttatcagccg ctaaggctaa tgctttatcc gttaagcttg ggtttaataa
     1501 agaatttacc cagccagctt ctcttgctga tatttataat caatcggagg acttttgtgt
     1561 tcaggataat caaagccagc cccttggatt taaattatcg tagccaattt gtattaataa
     1621 catagtttat tgcaagtatg gaaaaattac tggttcaaaa taccgatatc gctactgagg
     1681 aagttattcg caggttgaca caggaacaaa ttgagcagtt tacaattgtt cattgcagga
     1741 tttttagtat ttatccgacg aaaatgcgaa tctggccaag cacttttctg gtggaagaag
     1801 aggggaatcg tcgtaaactg ttgcataatt ttaatatctc tctttatcct ggatggactg
     1861 attattttgt caaaaatgat tatatcaggt ttacccttgt ttttgaagg
//
LOCUS       DAORLS010000454         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_31688_length_1908_cov_1.805720, whole genome shotgun sequence.
ACCESSION   DAORLS010000454 DAORLS010000000
VERSION     DAORLS010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31688_length_1908_cov_1.805720"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>393)
                     /locus_tag="PK977_19875"
     CDS             complement(<1..>393)
                     /locus_tag="PK977_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20453.1"
                     /translation="QALTLKDVYPNVDVRYYTSNGYLKYDIIVKPGGDVSKIALKYDG
                     VDKLQVKNKELLISTSIGELKESSPFTYQAELTGRKEINCKYLLKDNVLRFDVKDYDP
                     TKTLVIDPVIVFCSFSGSQADNWGFTAT"
     assembly_gap    394..416
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<417..880)
                     /locus_tag="PK977_19880"
     CDS             complement(<417..880)
                     /locus_tag="PK977_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20454.1"
                     /translation="MKRHLLTQTFKPTQAKISYFKKNNLTLKQTITLTVLLLSAFFAG
                     AQGNIEFIENKGQWDASVQYRGAVSNGDIFIRQGGGFTIVQHNPTDYQNVAKFLHGQN
                     PDNTPVTANDKIVLRSHAWNVDFVGASSASTIVADKMISTHNNYFIGNDPSK"
     gene            complement(921..>1908)
                     /locus_tag="PK977_19885"
     CDS             complement(921..>1908)
                     /locus_tag="PK977_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRF20455.1"
                     /translation="TIKILIGTHALIEDAVQFHNLGIVIIDEQHRFGVAQRAKLWQKA
                     AVPPHILVMTATPIPRTLAMTAYGDLDYSVIDEMPPGRQPITTVHRYESHRSKVMDFI
                     RTEIDKGRQVYIIFPLIEESPKLDYENLMKGYENVKAYFPEPKYWISMVHGRQPAEQK
                     EANMKRFVEHDTQIMVSTTVIEVGVNVPNASVMVIESAEKFGLSQLHQLRGRVGRGAE
                     KSYCILLTGSKLGNDARERIRIMTETNNGFVIAEKDLELRGPGDIEGTRQSGALNFRL
                     ASIVQDRPLLEVARNMAAKLLEEDPDLVSAENLRLKEFLHYQKGNVVWSRIS"
BASE COUNT          493 a          447 c          375 g          570 t
ORIGIN      
        1 atgtagctgt aaagccccag ttatcagcct ggctgcccga gaatgaacaa aagacaataa
       61 ccgggtcaat taccaatgtt ttggtgggat cgtaatcttt tacatcaaag cgaagcacat
      121 tgtctttaag caagtacttg cagtttattt cttttcgccc ggtcaattcg gcctggtagg
      181 taaacggtga tgactctttt aattctccaa tggaagtgga aatgagcagc tctttattct
      241 tcacctgcag tttgtcaaca ccatcatact tcaaagcgat cttggaaaca tcaccaccgg
      301 gctttacgat aatatcgtat ttcaggtaac cattgctggt ataatagcga acatccacat
      361 tggggtagac atccttcagt gttaatgcct ggannnnnnn nnnnnnnnnn nnnnnncatt
      421 tggaaggatc attaccgata aaatagttgt tatgagtact gatcatttta tccgcaacga
      481 ttgtgcttgc agaagatgcc cctacaaaat ccacattcca tgcatgtgaa cgtaagacga
      541 tcttatcatt ggctgttacc ggggtgttat caggattctg gccatgcaga aatttagcca
      601 cattctggta atcggtggga ttatgttgta caatggtaaa acctccacct tggcgaatga
      661 aaatatcacc attgcttaca gcacccctgt actgcacact ggcatcccac tggcctttgt
      721 tttcaataaa ttctatgttt ccctgcgcac ctgcaaaaaa tgctgaaaga aggagtacag
      781 ttaacgtaat ggtctgtttc agggtcaaat tatttttctt aaaataacta atttttgcct
      841 gagttggttt gaaagtttgt gttaataaat gtcttttcat ggtaaaattt cccaatcagg
      901 taaatacatc cattagtaaa ttacgaaatc ctgctccaaa ccacattgcc tttttggtaa
      961 tgaaggaatt cttttaaccg caaattttct gccgaaacga ggtccgggtc ctcttccagc
     1021 aattttgcag ccatatttct ggctacttcc agtaaaggcc tgtcttggac tatgctggcc
     1081 agccggaagt ttaaggctcc gctttgtctt gttccctcaa tatcgcccgg ccctctcaac
     1141 tcaagatcct tttcggctat tacaaacccg ttattggttt ccgtcattat cctgatgcgt
     1201 tcccttgcat cattacccag ttttgaaccg gtcagcaata tgcaataact tttctccgcc
     1261 ccccggccca cccttccccg cagctggtgc aattgggaca aaccaaactt ttccgcactt
     1321 tcaattacca taaccgaggc attgggcaca ttcaccccta cctcgataac cgtagtggaa
     1381 accatgatct gtgtgtcatg ctccacaaat cgtttcatat tggcttcttt ttgttcagcc
     1441 ggctgacggc catgtaccat actgatccag tatttaggtt ccggaaagta agccttcaca
     1501 ttttcatagc ccttcatcag gttttcataa tcaagcttag gactttcttc aattaaagga
     1561 aaaatgatat acacctgccg gcctttatca atctcagttc ttataaaatc catcactttg
     1621 ctccggtgcg attcataacg gtgaaccgtg gtgatgggtt gccgtcccgg gggcatttca
     1681 tcgatcacac tgtagtcaag atcgccgtaa gctgtcatgg ccagtgtacg gggaatgggt
     1741 gtggccgtca tgacaagaat atgcggaggc acggctgctt tttgccataa ctttgctctt
     1801 tgtgccacgc caaagcgatg ctgttcatca ataatcacaa tacccagatt atgaaactga
     1861 acggcatctt caatcagtgc atgggtgccg atcaaaattt ttatagtg
//
LOCUS       DAORLS010000455         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_31864_length_1899_cov_1.733731, whole genome shotgun sequence.
ACCESSION   DAORLS010000455 DAORLS010000000
VERSION     DAORLS010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31864_length_1899_cov_1.733731"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    237..376
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            447..1886
                     /locus_tag="PK977_19890"
     CDS             447..1886
                     /locus_tag="PK977_19890"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRF20456.1"
                     /translation="MSKIVRYCYLLSVFLLLPISATDAFETGQIQKRLVSLGFDSGPV
                     DGIPGSKTRSAIEQFQKKNNLPATGLVDQETEEYLFPDLVLYTQPFEPFHYPHPLTGK
                     LAGPAVELIQRACRDAMLRCVLKLGEGTWKETQEKVKAGSGDGLFLIGWNKERAEYLQ
                     STENILNTEYGFFARSTNHLAQKYTDISDLKGYVIGVYGPSNTSNTLDKLLLHLRDKG
                     YSADKYQTDDDDDVFRKFKETNFENYIVFSNRDVGNSIIKRLGLEDIRYIGRYRCVSY
                     VVGITTRHNSIIIQKFNTALRKLEENGYKQAIFAPYDISLSCGESLKYQQAPVSLPDP
                     GPLPSPAKKCDVKVINEKSIVECSDTCLQWQQSGSESVMSLAEANGYIRQLNANQFAS
                     FNDWRLPTADEFKTLFTQEGSGPNSLPIPEAFNTLQVDCWTSDQESTGKVIYVDFLKN
                     AFASKNADSFNYVRAVRGKSCQGNHGDND"
BASE COUNT          534 a          361 c          389 g          475 t
ORIGIN      
        1 cgtgctggaa cgcaggcccg ctatttttgg ggggattcta cagaccaagt caaaatatat
       61 gcgcattctt acataatggg ggaacctcta cccgaacatc ccatgaagcg tggaggcttt
      121 aagccaaatc agtggggaat ttttgatatt cttggaaatg tgagcgagtg gacttgttcc
      181 agacacacaa cgccacttga gaaagaggat acatcaaaaa atacttgtat tgacaannnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnngccg aggatttata ataagcggcc agaaaagaag tgtcgatgaa
      421 cccctgtttt ttggaggaat acatcaatgt caaaaatagt tcgttactgt tatttattat
      481 ctgtttttct tcttctgcct atcagcgcta ctgatgcatt tgaaaccggc caaatacaga
      541 agcgcctcgt ttctctgggc ttcgattctg gccctgttga tggtatacca ggatcaaaaa
      601 cccgatcggc aattgagcag tttcaaaaga aaaataatct acccgctaca ggtctggttg
      661 atcaagaaac agaagaatac ctattcccag accttgttct gtatacccag ccttttgaac
      721 cattccatta tccccatcca ttaacaggca agttagccgg accagcggtc gaattaatac
      781 aaagagcctg ccgtgacgct atgctacgct gtgtactaaa gttaggtgaa ggaacttgga
      841 aagaaacgca agagaaagtt aaagccggaa gtggtgatgg actctttttg attggctgga
      901 ataaagagcg tgcggaatat ctgcaaagta cagaaaacat actaaacact gaatatggct
      961 tcttcgctcg aagcactaac catctcgctc aaaagtatac cgatatcagc gatctgaaag
     1021 gctatgttat aggtgtttac ggaccttcta atacatcgaa tactctcgat aaattattgc
     1081 tgcatttgag ggacaaaggt tatagtgcgg ataaatacca aactgatgat gatgatgatg
     1141 tttttcgtaa gtttaaagaa actaactttg aaaattacat agtcttttct aaccgggatg
     1201 tcggtaactc aattatcaag cggctgggac tggaagatat caggtatata ggccggtacc
     1261 ggtgcgttag ctacgttgtg gggatcacga cacggcacaa cagtataatt atccagaaat
     1321 ttaatactgc tttacgcaaa cttgaggaaa atggttacaa acaagcgatt tttgctcctt
     1381 acgatatttc cctctcttgt ggagagtcct tgaagtatca acaagccccc gtttctttac
     1441 ccgatcctgg cccattacct tctccggcaa aaaaatgcga tgttaaggtt atcaatgaaa
     1501 aatcgatcgt tgaatgcagc gatacatgtc tgcaatggca gcaatcaggt tcagaaagtg
     1561 tgatgagctt ggcagaggcc aacggctaca tacgtcaatt gaacgccaat caatttgcat
     1621 cgttcaatga ttggcgtcta cccacagccg atgaatttaa aaccttgttt actcaggaag
     1681 ggtcaggacc aaatagcctg cctatccctg aggcgttcaa tacccttcag gtagattgct
     1741 ggacttcaga tcaagagagc actggcaaag tgatatatgt ggattttctt aagaacgctt
     1801 ttgcttctaa gaacgcagat agctttaatt atgtgcgggc agtacggggg aaatcctgcc
     1861 aaggtaatca tggtgataat gactgaaata atcatcatc
//
LOCUS       DAORLS010000456         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_32109_length_1887_cov_1.866266, whole genome shotgun sequence.
ACCESSION   DAORLS010000456 DAORLS010000000
VERSION     DAORLS010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32109_length_1887_cov_1.866266"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK977_19895"
     CDS             complement(<1..506)
                     /locus_tag="PK977_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRF20457.1"
                     /translation="MRIALAQQNYHIGNFESNTQKIINGIEKAKQSGADLVVFSELCV
                     CGYPPRDFLEFEDFINKCYESVDRIREYADGIGVLIGAPDKNPVREGKDLFNAAFLLY
                     DKQIKGVAHKTCLPNYDVFDEYRYFEPAYNWQVMEFKGKKLAVTICEDIWNLGDNPLY
                     RNCPMEEL"
     gene            584..1618
                     /locus_tag="PK977_19900"
     CDS             584..1618
                     /locus_tag="PK977_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20458.1"
                     /translation="MKKWIVYSGLFFFLFSCINDGKDIPDISGIKADVSIKRFDRDIA
                     NLDSNNVAAGLNELYQQNPQLTTIFLENILGLDSASTLTGVKNFLTLSQNLFDTINIV
                     FKDTKDLEKQFQEAFRYVKYYYPQYQVPVVYTVAGPMDALAQSDYGLTPNFLRPGIMG
                     ISLQFYLGKDFSVYQDPFFIQNVAPLYRSRRFSKEYIIADAIQLVVNDLFPDQSSGKP
                     LIEQMVEKGKQWYLLDKFLPTTSDSVKTGYTQLQLDWCSKNEGMIWTYIIKNEDLQSL
                     NPAVLQTYIGEAPFTQGMPQDYSPGNIGQWIGWQIVKKFAEKNSALKPEDIMKTPAAK
                     IIEEAKYKPK"
BASE COUNT          577 a          424 c          345 g          541 t
ORIGIN      
        1 attagttcct ccatgggaca gttacggtac aacgggttat cacccaggtt ccagatatct
       61 tcacaaatgg taacagccag ttttttacct ttaaactcca ttacctgcca gttatatgcc
      121 ggctcgaaat agcggtattc atcaaacaca tcataattgg gcaaacaggt tttatgggcc
      181 actcccttaa tttgcttatc atacagaaga aaggcagcat tgaaaaggtc tttaccctcc
      241 cttacaggat tcttatccgg agccccaatc aatacaccga tgccatcagc atactccctg
      301 atccggtcaa ccgactcata acacttattg ataaaatctt caaactcgag aaaatctctt
      361 ggtggatagc cgcaaacaca tagctctgaa aaaacaacaa ggtcagctcc cgattgttta
      421 gctttctcaa tcccgttgat gatcttttgg gtattacttt cgaagttgcc aatatgatag
      481 ttctgctgtg ctaaagcgat cctcatcggg cgaagttagt taatgttttt gtagcttact
      541 tttgctacac aatagtcaat ttgcataatc gtttaccatg tctatgaaaa aatggattgt
      601 ttactccggt ctcttcttct ttttattcag ttgcattaac gatggaaaag acatacctga
      661 tatttcagga ataaaagcag atgtaagtat aaaacgtttc gacagggaca tagcaaatct
      721 tgacagtaat aatgtggcag ccgggctgaa tgaactttac cagcagaatc ctcagcttac
      781 cactattttc ctggaaaata ttctgggtct tgacagcgcc tctaccctaa ccggcgtaaa
      841 aaacttctta accctgagtc agaatttatt tgacacaatc aatattgttt ttaaggatac
      901 aaaggacttg gaaaagcaat ttcaggaggc tttccgatat gtaaaatatt attacccgca
      961 ataccaggtt ccggtggttt ataccgttgc cggccctatg gatgcactgg cacaatctga
     1021 ttatggcctg acccccaatt ttcttcgccc gggaattatg ggtataagtc tccagtttta
     1081 cctcggaaag gatttttctg tataccagga tccgttcttt atacagaatg tagccccttt
     1141 ataccgaagc cgccgcttca gcaaggaata tatcattgct gacgccatac aactggtggt
     1201 gaacgatctt ttccctgacc aaagcagcgg caaaccactg attgagcaaa tggtagaaaa
     1261 aggaaaacaa tggtacctgt tggataaatt tctgcccaca acttctgatt cggttaaaac
     1321 cggctataca caactgcaat tagactggtg ttctaaaaac gaaggcatga tatggaccta
     1381 tatcataaaa aacgaagacc tgcaatcgct taaccctgct gttttacaaa cctacattgg
     1441 tgaagcccct tttacacaag gcatgccaca ggattattca cccggtaata tcggtcagtg
     1501 gatcggctgg caaatcgtaa aaaaatttgc agaaaaaaac agtgccctga agccggaaga
     1561 tatcatgaaa actcctgcag ctaagattat tgaagaggcg aaatacaaac caaagtgaat
     1621 aataaatgct gctgcctata atattttttc caggtaaaac tacttcataa taattgttgt
     1681 atttattaca tagatttaag gccatatgaa gctccttccc accaccctaa ccgatccggt
     1741 ctacacagca ccaaaaaaat attcgttcta cgaaaagtgc tggctcagga tcatgaatga
     1801 taaaagggac ctgcccttta tccacctgct tacaaaaatt catttatcag ttttgccggt
     1861 tgccattttg ctttttacaa accttct
//
LOCUS       DAORLS010000457         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_32204_length_1882_cov_1.649699, whole genome shotgun sequence.
ACCESSION   DAORLS010000457 DAORLS010000000
VERSION     DAORLS010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32204_length_1882_cov_1.649699"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PK977_19905"
     CDS             <1..1033
                     /locus_tag="PK977_19905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF20459.1"
                     /translation="PPADPLKGFLLLQDSSNLTYGHGFVNFSIKPVSNAQTLDSILAR
                     ADIVFDANDTIPTNIEKNTIDALPPTSQMGSLPTNSTNPLTLNWSGTDDANGCGVHFY
                     TLYISDDGVNFSILKDRMIRTDTTLPLTPNIRYYFFVLATDSVGNRETLRPGEIKSTF
                     IGVALPVTWLYFRGTNRNKDNILEWATANEQNTKEFKVERSLDGNSFNDIGSVTAAGN
                     SNTSQTYTYTDRNIDRLNSSVMYYRLRQVDLDNNYRYSSIIRLTYKEKEAKHSIVYPN
                     PASTIVTIAVGDKKLIGSVAAVFDMSGKLLENFTINGESQTFDISKYMNGIYFIRLEN
                     KETLRIVKQ"
     gene            complement(1135..>1497)
                     /locus_tag="PK977_19910"
     CDS             complement(1135..>1497)
                     /locus_tag="PK977_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[acyl-carrier-protein] S-malonyltransferase"
                     /protein_id="HRF20460.1"
                     /translation="ACERMKAAGAKRALVLPVGGAFHSPLMLPAKEELQAAILSTKFH
                     NPVCAIYQNVAAKAVMDQEEIKQNLIDQLTGAVRWTQSVQAMIADGASKFTECGPGKV
                     LQGLVLKINKEMQVDGVN"
     assembly_gap    1498..1601
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1602..>1882)
                     /locus_tag="PK977_19915"
     CDS             complement(<1602..>1882)
                     /locus_tag="PK977_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HRF20461.1"
                     /translation="GTEEDLKQTKVTQPAIFLHSVIAFRSIENAKPDMVAGHSLGEFT
                     ALVANGTLSFEDALSLVSVRATAMQKACEINTSTMAAVLNLSDDKVEAI"
BASE COUNT          514 a          403 c          355 g          506 t
ORIGIN      
        1 gccacctgct gatccgctaa aaggcttctt attattacaa gatagcagta acctgactta
       61 tggacatgga tttgtaaact tcagcattaa accagtttcg aatgctcaaa ctcttgatag
      121 tatcctggca agagcagaca ttgtttttga tgccaatgat accattccca caaatattga
      181 aaagaatact attgatgcac tgccacccac cagccagatg ggaagcctgc ccacaaacag
      241 taccaacccg ctgacactga actggtctgg tacagatgat gcaaatggct gcggggtaca
      301 cttttatacg ttatatattt ctgatgatgg tgtcaatttc agtatcctga aagaccggat
      361 gatccgcacc gatactactc ttccacttac acccaatatc aggtactact tctttgtgct
      421 ggcaacagac agtgttggca accgggaaac cctgcgaccg ggtgaaataa agagcacatt
      481 tatcggtgta gcattacctg taacctggtt atacttcagg ggaactaacc gtaataaaga
      541 caatatactt gaatgggcta ctgctaatga acagaatact aaagaattta aagtagaacg
      601 ttcgctggat ggtaatagtt tcaatgatat aggttcagta actgctgccg gcaacagcaa
      661 tactagccaa acatatacat acacagacag aaacattgac cggctaaata gttcagtgat
      721 gtattaccgg cttcgccagg ttgatcttga caacaactac cgctattctt ccattatccg
      781 gctaacgtat aaggaaaagg aagcaaagca ttctattgtt tatcctaatc cggccagcac
      841 catagttacg attgcagtag gtgataaaaa actgatcggt tccgttgctg cagtctttga
      901 tatgagcggt aaactgttgg aaaactttac cattaatggt gaaagccaga cattcgatat
      961 cagtaagtat atgaatggca tctatttcat ccggttggaa aacaaagaaa ctttaagaat
     1021 tgtaaaacag tgaggtcttc atatagtgtc gtttcatcag cctccttttt catgcaagaa
     1081 agaaatcccc cggtttatac cgggggatta agttttatca aagaggtata tacatcagtt
     1141 taccccatcc acctgcattt ctttattgat cttaagcaca agaccctgca atactttacc
     1201 aggaccacat tcagtgaatt ttgaggcacc atccgcaatc atggcctgaa cactttgtgt
     1261 ccatcttacc gcacctgtca gttgatcgat aaggttttgc tttatttctt cctggtccat
     1321 cactgctttg gcggctacgt tctggtaaat ggcgcaaacc gggttatgaa attttgtaga
     1381 aagtattgcc gcctgcaatt cttctttcgc cggcagcatc aaaggtgaat gaaatgcgcc
     1441 gcctaccggt aacaccaatg ctcttttagc tcctgcagct ttcattcttt cacaggcnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaatagctt ctaccttgtc
     1621 atctgacagg ttcaatacag ccgccatagt ggaagtattt atttcgcagg ccttttgcat
     1681 tgccgtggcc cttacagaaa cgaggctgag ggcatcttca aaacttaatg tgccattagc
     1741 caccagggcg gtaaattctc ccaaagaatg accggccacc atgtccggct ttgcattctc
     1801 aatacttcta aaagcaatta ctgaatgtaa aaagattgca ggttgtgtaa ctttcgtttg
     1861 cttcaggtct tcttcggttc ct
//
LOCUS       DAORLS010000458         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_32384_length_1873_cov_1.113311, whole genome shotgun sequence.
ACCESSION   DAORLS010000458 DAORLS010000000
VERSION     DAORLS010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32384_length_1873_cov_1.113311"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK977_19920"
     CDS             complement(<1..280)
                     /locus_tag="PK977_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20462.1"
                     /translation="MIKKISLCWIAIMAVVNSQAQPNQIPDSVIIEICTAIKENKSTD
                     DSVRVFSAFERHLFPILRVLDDSTGTEAFIYTYYRLQKLCPEFEKIINQ"
     gene            complement(299..>726)
                     /locus_tag="PK977_19925"
     CDS             complement(299..>726)
                     /locus_tag="PK977_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216706.1"
                     /note="ATP-dependent carboxylate-amine ligase; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRF20463.1"
                     /translation="NTVEFRVCDIPLTVDETITLAALFQAICARIYMLRSKNLNFIQY
                     SRALLNENKWRASRYGVDGYLIDFGKEEEVNTRALIYELLDFLDPVLDHLGSRHRIAY
                     VNKILEHGTGADRQLAVFEKTRNLSDVAKYINSQYLVGI"
     assembly_gap    727..843
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<844..1413)
                     /locus_tag="PK977_19930"
     CDS             complement(<844..1413)
                     /locus_tag="PK977_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HRF20464.1"
                     /translation="MTLNYKTFTLGVEEEYMVLDPNTFELKSHEQRIVQEGQKMIKDK
                     VKAEMHQAVVEVGTDICADIDEAFHDVAALRKTIHGIADGLGFAMGAAGTHPFSHWES
                     QLITDHIRYNEIVNELQEAARSNLIFGLHVHVGMESREMANHIANSTRYFLPHVFALS
                     TNSPFWEGRMTGYKSFRTKVFDKFPRTGIP"
     assembly_gap    1437..1622
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1623..1858)
                     /locus_tag="PK977_19935"
     CDS             complement(<1623..1858)
                     /locus_tag="PK977_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF20465.1"
                     /translation="MSYRKLEVWQLAREIVIEIHKMSLSLPKFEMFEEGQQIRRSSKS
                     VKSTIVEGYGRRRYKNEYIKFLVYAHASNDETID"
BASE COUNT          395 a          365 c          338 g          472 t
ORIGIN      
        1 cctggtttat tattttctcg aattcaggac acagtttttg cagcctgtag taagtgtata
       61 tgaaagcttc tgtaccggtc gaatcatcaa gtacccttag tatggggaat aggtgccttt
      121 caaacgcaga gaatactctc actgaatcat cagtgctctt attctcttta atggcggtac
      181 aaatttcaat tataactgaa tccggtatct gattcggttg agcttggcta ttaacaactg
      241 ccattatagc aatccaacaa agtgagattt tttttatcat ggattactta tccttttttt
      301 aaatcccaac caggtattga ctgttaatgt attttgctac atcactgagg tttcttgttt
      361 tttcaaacac ggccagctgg cggtcggcgc cggtgccatg ttccagtatc ttgtttacat
      421 aggcaatgcg gtgacggctg ccgagatgat caagtaccgg gtcgaggaaa tccagcaact
      481 cataaataag ggcccttgta ttcacttctt cttccttgcc aaaatcaatc aggtagccat
      541 ccacaccata gcggctggcc ctccatttat tttcattgag cagggcacgg ctgtactgga
      601 taaagttcag gttcttggac cgcagcatgt agatgcgggc acatatggcc tggaacagcg
      661 ccgccagggt aatggtttca tccaccgtca gcggtatatc acataccctg aactcaaccg
      721 tattaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnngggtatg ccggtacggg gaaacttgtc aaatactttg gtgcggaaag atttgtagcc
      901 agtcatcctc ccttcccaga aaggggagtt ggtgctcagt gcaaatacat gcggcagaaa
      961 ataacgggtg ctgttagcaa tatggttggc catctcccgg ctctccatac ctacatgcac
     1021 atgcaggcca aagatgaggt tgctccttgc tgcttcctgc aattcgttta caatttcatt
     1081 gtaacggata tgatcggtga tcagctggct ctcccagtga ctgaaaggat gtgtaccggc
     1141 tgcacccatg gcaaagccca aaccatcggc aataccatga atggttttgc gcagtgctgc
     1201 cacatcatga aaggcttcat caatatcggc acagatatca gtgcctactt ctaccacggc
     1261 ctggtgcatc tccgccttta ctttgtcttt gatcatcttc tggccttcct gcacaatgcg
     1321 ctgctcatgg cttttcagtt caaaagtgtt cgggtcaagt accatgtact cttcttccac
     1381 gccaagggta aaggttttgt aattaagggt cataagtaat gttgataggt tgataannnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nntggtcgat ggtttcatca ttggaagcat gagcataaac caggaactta atatactcat
     1681 tcttgtaacg tcttcgtcca taaccttcca caatagttga ctttacagat ttgcttgatc
     1741 gcctgatttg ttgcccttct tcaaacattt caaattttgg gagcgacaaa ctcatcttat
     1801 gaatttcaat gacgatttcc cgggccagtt gccagacctc cagctttctg taactcattg
     1861 tataaggttt aaa
//
LOCUS       DAORLS010000459         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_32544_length_1865_cov_1.666298, whole genome shotgun sequence.
ACCESSION   DAORLS010000459 DAORLS010000000
VERSION     DAORLS010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32544_length_1865_cov_1.666298"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PK977_19940"
     CDS             <1..311
                     /locus_tag="PK977_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRF20466.1"
                     /translation="GTLAERRYTDDVPMEVKKRRLQEIVELQNRLSQESNKKDLGKTF
                     KVLIEGDSKRSDKDWMGRNSQNKVVVFAKENYALKKGDYVNVKVEDCTQATLLGKIVK
                     "
     gene            378..>677
                     /locus_tag="PK977_19945"
     CDS             378..>677
                     /locus_tag="PK977_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRF20467.1"
                     /translation="MDIQSIKNRFGIIGNSPALNYALQVASQVANTDLTVLINGESGV
                     GKEVFSQIIHSLSARKHNPFIAVNCGAIPEGTIDSELFGHEKGSFTGALTTKLGHF"
     assembly_gap    678..687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <688..>964
                     /locus_tag="PK977_19950"
     CDS             <688..>964
                     /locus_tag="PK977_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRF20468.1"
                     /translation="AVDARKGYFETVNGGTIFLDEIGELPLGTQARLLRVLETGEFIR
                     VGSSKVQKTDVRVIAATNKDLLQLVQAGKFREDLYYRLSTVPIRVPAL"
     assembly_gap    965..1033
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1034..1689
                     /locus_tag="PK977_19955"
     CDS             <1034..1689
                     /locus_tag="PK977_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF20469.1"
                     /translation="AERYKTPSVQLDDEAKDLLIHYPWPGNVRELKNIAEQISVLSQD
                     KLINAKTLNKFLQPYSNNRLPVLAHPNGSSTDFSNEREILYKLFFDMKKDVTELKRMF
                     LDILQNPNMVAQNQAFINELKESDNYRLKTNEVLQPVLVQPAVQHVQPAPVTIGMPGD
                     IDEHEEVEESLNIIDKEKELIIKALKKHKGKRKDAALDLGISERTLYRKLKEYDIEE"
BASE COUNT          585 a          344 c          415 g          442 t
ORIGIN      
        1 ccggcaccct cgcagaaaga aggtatacag atgatgtgcc catggaggtg aaaaaaagaa
       61 ggttacagga aatagtggaa ttgcagaaca ggctttcaca ggaaagtaat aaaaaagatc
      121 ttggtaaaac cttcaaagta ttgatagaag gtgatagtaa acggagcgac aaggactgga
      181 tgggaagaaa ttcacaaaat aaagtcgtgg tgtttgcaaa agaaaattat gcgttgaaga
      241 aaggcgatta tgtaaatgtg aaagttgaag attgtaccca ggcgacatta ctgggaaaaa
      301 ttgttaaata aaattgccac tgaaacacag aaccacagag atgtttcagt gtttcagtga
      361 atcagtggct taatactatg gatatacaaa gcattaaaaa ccgttttggc attattggca
      421 actcccctgc ccttaactat gcgttgcagg tggcatcaca ggtggctaat acggacctca
      481 cggtattgat taacggagaa agcggcgttg gtaaagaagt gttttctcaa atcattcact
      541 ccctttcggc aagaaagcat aatcctttta tagcagtaaa ctgcggcgcc ataccggaag
      601 gcaccataga ttccgaactc tttggtcatg agaaaggatc ttttaccggc gcgcttacca
      661 ccaagctggg gcattttnnn nnnnnnngca gtagatgccc gcaaaggcta tttcgaaaca
      721 gtgaacggcg gtactatttt cctggatgaa attggtgaac tgccattggg cactcaggcc
      781 cgtttgctca gggtacttga aaccggtgag ttcatccgtg tgggttcatc caaagtgcag
      841 aaaacggatg taagggtgat agctgctacc aataaggacc tgctgcaact ggtacaggcc
      901 ggaaagttca gggaggacct ttattatcgt ttaagcaccg tacctatcag ggtgccggct
      961 ttacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnntcgccga acgctataaa acaccttctg tgcagcttga tgatgaggcg
     1081 aaagatctgt taattcacta cccatggccc ggtaatgtgc gggaattgaa gaatattgca
     1141 gagcaaattt cagttctttc acaggataaa ctgatcaatg caaaaaccct gaataagttt
     1201 ttgcagcctt attccaataa ccggttaccg gtactggcac atcccaatgg ttcctcaact
     1261 gatttctcca atgaaagaga gatactgtac aaactctttt ttgatatgaa gaaagatgtt
     1321 acagaactga aaaggatgtt tctggatata ttgcaaaacc ccaatatggt agcacaaaat
     1381 caggctttca ttaatgaact taaggaatcg gataactacc ggttgaaaac aaatgaagta
     1441 ttacagccgg tactggtgca gccagctgtt cagcatgtac agcctgcacc agtcaccatc
     1501 ggtatgccgg gtgacattga tgaacatgaa gaagtggaag aaagcctcaa tatcattgat
     1561 aaggaaaaag aactgatcat taaagccctg aaaaaacaca aaggaaagcg aaaagatgct
     1621 gccctcgatt tgggcataag tgaaaggaca ctctacagga aattgaaaga atacgacatc
     1681 gaagaataat gaaaaaactc tttcgcaata ttttattacc agttgcagct ttgtttgtaa
     1741 tgggagctat tatcagctca accggctgta gtgtccgcta tggcttcaaa gatgtttcaa
     1801 tcccggatac tatcaagaca gtgaaagtgg gctatattga aaacagggca aggtatatca
     1861 accct
//
LOCUS       DAORLS010000460         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_33206_length_1836_cov_2.097136, whole genome shotgun sequence.
ACCESSION   DAORLS010000460 DAORLS010000000
VERSION     DAORLS010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33206_length_1836_cov_2.097136"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PK977_19960"
     CDS             complement(<1..999)
                     /locus_tag="PK977_19960"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRF20470.1"
                     /translation="MKTNNFLLSAAAVFITTTGCANDNPANTNRAEGDTSKLPPVETK
                     KANTNYKPAFAGQTRIAAVKTTTPYQVDKIAEKLGRPWAVVPMQDGRLLITEKSGYMM
                     ILDASGKLVKKITGFPAVDDRGQGGMLDVAFDPDYKKNKTIYWSYSEKYGEGNLMAVA
                     KGQLNEAAGKVDNPVVIFRATPALKSTLHYGSRLVFDKDGNLFVSTGERSILEGRVQA
                     QDLSSGLGKIFKITKDGKPAPGNPYINKPNAKPEIYAYGIRNAQSLDIHPTTGELWEA
                     EFGPRGGDELNIIKPGKNYGWPVITYGIEYGGKEIGNAIQQKNWMEQPVYYWDPVLS"
     gene            complement(1111..>1836)
                     /locus_tag="PK977_19965"
     CDS             complement(1111..>1836)
                     /locus_tag="PK977_19965"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF20471.1"
                     /translation="IPGTDKVLKNEYVIHSAHLDHVGIGRVVNGDSIYNGAHDNASGV
                     ASLLEIARIYKNSDAKPKRSILIVMVTGEEMGLIGSSYFAANPTVPKGSIVANVNTDM
                     PTVIAPLLSIVPLGAEHSTMMKHVQFAASQLGLEVENDPEPNENRFVRSDQYSFVLNG
                     IPALHIKYGTKTNVPGFDLVAFVKQWRAKYYHQSADGMDGIFDFAAAKVYVQLNFLVS
                     YSIAQTTARPVWNEGDLFGNAAN"
BASE COUNT          457 a          405 c          393 g          581 t
ORIGIN      
        1 agagagaaca ggatcccagt agtaaacagg ttgttccatc cagttttttt gctgaatagc
       61 attaccgatt tctttaccgc catattcgat tccatatgtg ataacaggcc acccgtaatt
      121 cttacctggt ttgatgatat ttagttcatc gccgccacgg gggccaaatt ctgcttccca
      181 taattcaccg gttgtggggt gaatatcgag gctttgtgca ttacggatgc cataagcata
      241 tatttcaggc ttagcattgg gtttattaat ataagggttg ccaggtgccg gcttaccatc
      301 tttggttatc ttgaatatct ttccaagacc agatgaaaga tcctgtgcct gtactctgcc
      361 ttctaatatt gacctttctc ctgtgctgac aaagaggttt ccgtctttat caaacaccag
      421 tcttgaacca taatgcaggg tgctttttaa tgccggtgtt gccctgaaga ttactaccgg
      481 gttatctact tttcctgctg cttcatttaa ctgaccctta gctacagcca tcaggttgcc
      541 ttcaccatac ttttcggaat aggaccaata aatggtttta ttttttttgt agtcaggatc
      601 aaatgcgaca tcgagcatac ctccctggcc tctgtcgtca acagcaggaa agccagtaat
      661 tttttttacc aatttaccac tggcatccag tatcatcatg tagccgcttt tttctgtaat
      721 aagcaggcgg ccatcctgca taggcaccac agcccagggt cttcccaatt tttctgctat
      781 tttgtctact tggtatgggg tagtggtttt tacagcggca atcctggttt gccccgcaaa
      841 cgcaggttta taattagtat ttgccttttt agtttcaaca ggtggtaatt tactggtatc
      901 gccttcagcc cggttagtat ttgctggatt gtcattggca caacctgttg tcgtgatgaa
      961 aactgctgct gcagataaga gaaaattatt tgtcttcata acaattattg atcgggtaaa
     1021 attagagaag tgatgggtat tgattctttt attacataag ccttacaaat tccttgacaa
     1081 aatgtttaag taaacgagga atggtatgtg tcaattggct gcatttccga acagatctcc
     1141 ttcgttccat acaggtcttg ctgttgtctg cgcaatggaa taactaacca ggaaattgag
     1201 ctgtacataa actttagcag ctgcaaaatc aaaaattccg tccattccgt cggcggactg
     1261 gtgataatat tttgctcgcc actgttttac gaatgcaacc aggtcgaagc cgggtacatt
     1321 ggttttcgtt ccatacttaa tatgcagggc aggtattcca ttcaatacaa agctatactg
     1381 gtcgctgcgt acaaaccggt tttcgttggg ttcaggatcg ttttctactt ccagtcctaa
     1441 ctggctggca gcaaattgta catgcttcat catggtgctg tgctctgccc cgagcggcac
     1501 tatcgataag agcggcgcaa tgactgttgg catatctgta ttaacattag ccactatgga
     1561 acctttgggc acggttgggt tggcagcgaa ataagacgac ccgataagcc ccatttcttc
     1621 gcctgttacc ataacaatta atatggagcg tttaggcttt gcgtctgaat ttttataaat
     1681 gcgggcaatt tccagcagtg aagctacccc cgatgcatta tcatgggcgc cattatatat
     1741 ggaatctccg tttacaacac ggccgatacc cacatgatcg agatgagcgg agtgaatcac
     1801 atactcgttt ttgaggactt tatctgtacc gggaat
//
LOCUS       DAORLS010000461         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_34248_length_1788_cov_1.828621, whole genome shotgun sequence.
ACCESSION   DAORLS010000461 DAORLS010000000
VERSION     DAORLS010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34248_length_1788_cov_1.828621"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK977_19970"
     CDS             complement(<1..272)
                     /locus_tag="PK977_19970"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRF20472.1"
                     /translation="MEKTLLVRDFLFVSKLSYGPRIPNTPLSVPFVHNYMPIGKGKSY
                     STALQLPYIRWFASKVKRGDAVVFNFPEGDTVYHAAGFESANPYYD"
     assembly_gap    289..331
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<332..>672)
                     /locus_tag="PK977_19975"
     CDS             complement(<332..>672)
                     /locus_tag="PK977_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HRF20473.1"
                     /translation="LAKLFAKAGEASWKAYIPFYNTWVMQDLAKRPKHWVFWQFIPVI
                     GWFISPGIFIEFAKVYGRFSLGSHTAAALGAPVYFPYLAYKVNPNFVGAEAVKKHKKP
                     GWREWVDAGIF"
     assembly_gap    673..698
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            871..1758
                     /locus_tag="PK977_19980"
     CDS             871..1758
                     /locus_tag="PK977_19980"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRF20474.1"
                     /translation="MNEFTIRDIENLCGIKAHTLRAWEQRYRLFVPKRKESQHRIFDD
                     NDLKELLRISFLYHNGYKVSKLAAMSAEEIQHAVTSTIVRDDNYELFVHQMIEASIGF
                     DKEALDKIINTIAIRIGMEKAITNVFYPFLKRIGLLWLSNNVIPAQEHFASHIIRKKI
                     IMATDGLEIPLGQESIILVFAPKGELHEIPLLAANYFFRKHNNPTVYLGVNIDLETLR
                     YYLDRKPADYIYTHIITHFDDEELDDYFCKLSREYPQKKILVSGPAACCIKKVPQNLT
                     VFHSIDEMLNFARNMAKTT"
BASE COUNT          547 a          367 c          341 g          464 t
ORIGIN      
        1 atatcataat aagggttggc cgactcaaag ccagcagcat gatacaccgt atcaccttca
       61 ggaaaattga agactaccgc atctcccctt tttaccttag aagcaaacca acggatatag
      121 ggcagttgca gcgcagttga atacgacttt cctttaccaa tgggcatgta attgtgtaca
      181 aaaggaacag ataagggtgt attaggaata cgggggccgt aacttagttt gctaacaaac
      241 aagaagtccc taaccagtaa ggttttttcc atagatcctg ttgggatcnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaaaaatgc cggcatccac ccattctctc
      361 catcccggtt tcttatgctt cttcacggcc tcggcaccaa cgaaatttgg atttacctta
      421 tatgcgaggt aaggaaaata aacaggtgcc cccaatgctg cagctgtatg gctgccaagt
      481 gagaacctcc catacacttt ggcaaactct ataaatatgc cgggactgat aaaccagcca
      541 atcacaggaa taaactgcca gaaaacccaa tgttttggtc ttttagccaa atcctgcatc
      601 acccaggtat tgtaaaaagg tatataagct ttccaggatg cttctccagc ctttgcgaat
      661 aatttagcca acnnnnnnnn nnnnnnnnnn nnnnnnnnga tcaatgtacc gatcaggtaa
      721 acgatgaaaa tatgatataa tggcatgagc cggtttttat agctgacaaa agtattataa
      781 atatttgccg ttcaaaattt taatattcaa agttttgcta aagcgcttca gggaattgcg
      841 gggaataaat gataatttag ggcctgggaa atgaacgaat ttaccatcag ggacatagaa
      901 aacctttgtg gcataaaagc ccacacactc cgggcatggg aacagcgtta ccgtttattt
      961 gtgcccaaac gcaaagaaag ccagcatcgc atttttgacg ataacgacct gaaagaattg
     1021 ctccgaatct ctttcctgta tcataatggt tataaagttt ccaaattagc tgcgatgagt
     1081 gcggaggaaa ttcagcatgc tgtgacttcc actatcgtca gggatgacaa ttacgaactc
     1141 tttgttcacc aaatgatcga agccagtatt ggctttgata aagaggcact ggataagatc
     1201 attaatacta ttgctatccg gatcggtatg gaaaaagcca taaccaatgt attctacccc
     1261 tttttaaaac gaattggcct gttatggctt tccaataatg tgatacctgc acaggaacat
     1321 tttgccagcc atataatccg taaaaagata atcatggcaa cagacgggct tgaaataccc
     1381 cttggccagg aaagtattat ccttgtattt gcaccgaaag gagaactgca cgaaattcca
     1441 ctgctggcag caaattattt tttccggaaa cataataatc caactgttta cctcggtgta
     1501 aacatcgacc tggaaacatt acggtattat ctcgacagaa agcctgcgga ttacatctac
     1561 acacatatta taacccattt tgatgatgaa gaattggatg attatttctg taagcttagc
     1621 agggaatatc cgcaaaaaaa gatactggtt tcaggacccg cagcctgttg catcaaaaaa
     1681 gtaccgcaga atcttaccgt atttcattca attgatgaaa tgctgaattt tgcccggaat
     1741 atggctaaaa ctacttaggc ataacatagc ttcttatatc ttcggcag
//
LOCUS       DAORLS010000462         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_34361_length_1783_cov_2.001736, whole genome shotgun sequence.
ACCESSION   DAORLS010000462 DAORLS010000000
VERSION     DAORLS010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34361_length_1783_cov_2.001736"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..>1783)
                     /locus_tag="PK977_19985"
     CDS             complement(14..>1783)
                     /locus_tag="PK977_19985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20475.1"
                     /translation="FRTKSPQLSTNEKLLVKGSSFIRYSTANKEKTGHADVSVGGKKL
                     GWLTSFTFSDFGDTKMGSNYPKRYPDFGRRSRYIETVGGIDYVMVNNDDRVQKFSGYK
                     QWDITNKFLFKQNEKVSHSLNLQYSNSTDVPRYDRLQDVRNFGGSIGTTLRWAEWYYG
                     PQKRLLGAYELNIEKAGFLDNLKVNLNYQDVKESRQQREYRRYDRFDSRREHIQVAAF
                     NIDGRKTWGLNELNMGIDGQFNDVKSVADRTNLTTGAVTKLDTRYPDGKNKMNFLGLY
                     VQHLLKLKSGKLIINDGIRFQYVSLHSTIVDNSFFNLPVTDIKQTPFAVTGNLGLVYM
                     PVSEFRLTGNISSGFRSPNIDDLARVFESNTASKRVIIPNSEIGPEYTYNFDFGLTYS
                     VPGIARFEMTAFYTLFRNAIALAPFTLNGLDSIIYNGVRSAVFANQNVNKAYVRGLNA
                     RMKINFSPKVSWDNTISQTFGRYVDEKGGKKPLDHVPPVFGKTAITYTGKKLSTELFC
                     LFNGWKKIKDYNPDGEDNGQYATPDGMPSWITVNWRGNYTMTKNLELQLGIENIFDRN
                     YRYFASGFSAPGRNFIIAIRANW"
BASE COUNT          464 a          383 c          356 g          580 t
ORIGIN      
        1 atgggggttg ttattaccag tttgcccgaa tggcaataat aaagtttctg ccgggggcgg
       61 aaaagcccga agcaaagtac cggtagtttc tgtcaaaaat attttctatt cctaattgta
      121 attccaggtt tttggtcatc gtatagttac ccctccagtt aacagtaatc caggaaggca
      181 tgccgtcagg agtggcatat tgaccattat cttctccgtc aggattatag tcttttattt
      241 ttttccatcc gttaaacagg caaaacaatt ctgtgcttag cttcttgcct gtatatgtga
      301 tagcagtttt gccaaaaaca ggcggtacat ggtcaagggg tttcttgccg cctttctcat
      361 ctacatacct gccaaatgtc tggctaatgg tgttatccca actgacttta gggctgaaat
      421 tgattttcat tcttgcattc aatcctctta catacgcttt gtttacattc tgattggcaa
      481 aaacagctga tcttactcca ttataaatga tggagtctag tccgtttagt gtaaacgggg
      541 ccaatgcaat agcattccgg aagagtgtgt aaaatgctgt catctcaaag cgggcaattc
      601 ccgggacaga ataagttaag ccgaaatcaa aattgtaagt gtattcaggg cctatttcac
      661 tattaggaat aataactctt ttgctcgcag tatttgattc aaataccctt gccaaatcat
      721 ctatgttggg cgatctgaat cctgatgaga tattacctgt aagccggaat tcactgactg
      781 gcatatatac taatcccagg tttccggtta ctgcgaaagg tgtttgtttt atatcagtca
      841 caggcaggtt aaaaaaggaa ttatcaacaa tggtagaatg aaggcttacg tactgaaacc
      901 tgattccgtc atttattatc agtttgcccg atttcaattt gagaaggtgc tgaacataca
      961 ggcccaggaa gttcatttta ttttttccat ccggatagcg ggtatcaagt ttggtaactg
     1021 ctcccgtagt gaggtttgtt cggtctgcca ctgatttcac atcattgaac tgtccgtcaa
     1081 tccccatgtt gagttcattt agtccccagg ttttacggcc gtcaatatta aaagcagcga
     1141 cctgaatatg ttcccgccgg ctgtcaaaac ggtcatagcg ccggtattct ctttgctgcc
     1201 tgctttcttt cacatcctgg taattcaggt ttactttcag gttatccagg aatcctgcct
     1261 tttcgatatt cagttcataa gcacccaata atcttttctg gggtccataa taccattcag
     1321 cccatcgaag ggtggtgcca atactgccgc cgaagtttct tacatcctga agacgatcat
     1381 accttggtac atctgtagag ttagagtatt ggaggttcag gctatgtgat actttttcgt
     1441 tctgcttaaa tagaaattta ttggttatat cccactgctt gtagccagag aacttttgta
     1501 ccctatcatc attgttgacc attacatagt ctatgccacc cactgtttca atgtagcggg
     1561 accttcttcc aaaatcagga taccttttag gataattact acccattttt gtatcgccga
     1621 agtcactgaa agtaaacgaa gtgagccaac ccagtttttt cccgcccaca ctcacatctg
     1681 catgaccggt tttttcttta ttggcagtgc tataccgtat aaaagatgat cctttcacca
     1741 ataatttttc attggtactc agttgtggtg atttggtacg aaa
//
LOCUS       DAORLS010000463         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_34509_length_1777_cov_2.250871, whole genome shotgun sequence.
ACCESSION   DAORLS010000463 DAORLS010000000
VERSION     DAORLS010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34509_length_1777_cov_2.250871"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK977_19990"
     CDS             <1..275
                     /locus_tag="PK977_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20476.1"
                     /translation="YLSNVSYRFGLFAGPDYIQVGRKLPQFGGSFGLGLPLATSRQAP
                     NQATLINLAFEYSKRGNNDNLLKENMFRVSVGFSVSDFWFIKRKYD"
     gene            268..846
                     /gene="lptC"
                     /locus_tag="PK977_19995"
     CDS             268..846
                     /gene="lptC"
                     /locus_tag="PK977_19995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRF20477.1"
                     /translation="MINYPFSIKSLITAAAFFTGCCLLLSCENDEKKVKEWTENKVMK
                     EEVTGVESYLSQDGKMRAKLTSPLMLRVMADTVYVEFPKSIHVDFYNDSAVIESKLDS
                     KYAKYFENLNKVYLRDSVVVITMKGDTLKSPDLWWDQNTKMFYTEKYATYHGIGKQIY
                     GGKGLTATQDLSSVIFNQPTGTVQVSEEGFPK"
     gene            926..>1777
                     /locus_tag="PK977_20000"
     CDS             926..>1777
                     /locus_tag="PK977_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF20478.1"
                     /translation="MEYRRMGKTGLQLSVLSFGSWVSFHKQINDSSADELMGIAYDNG
                     VNFFDNAEVYALGESEKMMGRVLKKKNWDRTSYTVSSKAFWGWRGKDNKPNQTGCSRK
                     HLTEACNEALQRLQLDYLDLFFCHRPDKGTPIEETVWTMHNLIQQGKILYWGTSEWSG
                     VEIMEAHRVAHQYGLIGPAMEQPQYNLLERNKMENEFLMIFKTVGMGTTIWSPLASGL
                     LSGKYNDGIPEGSRFGLTGFDWLKDRWMKDDLLNKVRQLGTLANELGISQAELSIAWC
                     IKNPNVTT"
BASE COUNT          549 a          325 c          442 g          461 t
ORIGIN      
        1 attatttaag caatgtttct taccgctttg gattatttgc aggaccagat tatatacagg
       61 taggacggaa acttccccag ttcggcggaa gttttggact gggtttaccg ctggctacca
      121 gcaggcaagc tcctaaccag gcaacactta tcaacctggc atttgaatac agcaagcggg
      181 gaaataatga taatttgcta aaggaaaata tgttcagggt atcggttgga ttttcagtca
      241 gcgacttctg gtttatcaag cggaaatatg attaattatc ctttctctat aaaatcatta
      301 ataacagcag cagctttttt tacaggctgc tgtttgcttt tgagctgtga gaacgatgag
      361 aaaaaagtga aggaatggac agagaacaaa gtgatgaaag aagaagtgac cggtgttgaa
      421 agttatttaa gccaggatgg aaaaatgagg gccaaactaa cttcgccact gatgctgcgg
      481 gtaatggcag atacagtgta tgtagaattt cctaaaagta tacatgttga tttttacaac
      541 gacagtgctg ttatagaaag caagttagac agcaagtacg ccaaatattt tgagaacctg
      601 aacaaagttt acctgcggga cagtgtagtg gtgattacca tgaaaggcga tacattgaaa
      661 agccccgacc tgtggtggga ccagaatact aaaatgtttt ataccgaaaa gtatgctact
      721 tatcatggca tcggtaagca gatttacggc ggaaaaggcc tgacggctac acaagacctc
      781 agcagtgtta tcttcaacca gccaacggga accgtgcagg tttctgaaga aggttttccc
      841 aaataaactt tttaacggct ttaactgttc aagtaccttt aggttgctaa aagcaagatg
      901 ttttaaatat taataatcaa tcaaaatgga atacaggaga atgggtaaaa ccgggcttca
      961 gctgagtgtg ctttcttttg gcagctgggt cagttttcat aagcagataa acgacagtag
     1021 tgcggatgaa ctaatgggca ttgcgtatga taatggtgtg aacttttttg ataatgctga
     1081 agtgtatgca ttgggtgaaa gtgagaaaat gatgggccgt gttttgaaaa agaaaaactg
     1141 ggaccgtacc tcttataccg tttcttccaa agctttctgg ggatggagag gaaaagataa
     1201 caaacccaac cagacaggct gcagcaggaa acatttgaca gaagcctgta acgaagcttt
     1261 acaacgcctg caattggatt accttgatct gtttttctgc caccggccgg ataaaggcac
     1321 accgatagaa gaaacagtgt ggacgatgca taacctgatt cagcagggca aaatattata
     1381 ctggggtacc agtgagtgga gtggtgtgga aattatggag gcacaccggg tggcccatca
     1441 gtacggactg atcggtcccg ccatggagca gccgcaatac aatttgctgg agcgaaataa
     1501 aatggaaaat gagttcctga tgatttttaa aacagtgggt atgggtacta ccatctggag
     1561 tccgctggca tcaggcttac tcagtggtaa atacaatgat ggtattcccg aaggtagccg
     1621 ctttggcctt actggtttcg actggctgaa agaccgctgg atgaaagatg acctgctgaa
     1681 caaagtccgt caattgggta cccttgccaa tgagcttggc atctcacagg cggaactgag
     1741 tattgcctgg tgtattaaaa acccgaatgt aacaaca
//
LOCUS       DAORLS010000464         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_34596_length_1774_cov_1.764398, whole genome shotgun sequence.
ACCESSION   DAORLS010000464 DAORLS010000000
VERSION     DAORLS010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34596_length_1774_cov_1.764398"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..>694
                     /locus_tag="PK977_20005"
     CDS             163..>694
                     /locus_tag="PK977_20005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF20479.1"
                     /translation="MQLLSVVIVCKNEAGIIGTTLQSLQGLTDDIVVYDNGSTDDTLV
                     IAQQYATNVIRGEWEGFGKTKQKAVALAKHDWILSLDADESIDDELKNELLRLPLNDE
                     NVVYEIKFKNFFGNQWLKYGEWGNDSHTRLFNRQKVNWNEAPVHEGLVLPGGIQKKRL
                     KGYVLHYTATDIQRYKT"
     assembly_gap    695..706
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <707..944
                     /locus_tag="PK977_20010"
     CDS             <707..944
                     /locus_tag="PK977_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20480.1"
                     /translation="MKYAALNAEKYFEQGKKIGWFKKYISAFFSFTQNYIFRAGFLDG
                     KAGYRCAMLTATYTFNKYKNLEQLIRAATETLKH"
     gene            941..1666
                     /locus_tag="PK977_20015"
     CDS             941..1666
                     /locus_tag="PK977_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRF20481.1"
                     /translation="MKKSVILCFSGYSLLSTIFLLFPNLQLKALTNFIPIFPLGIVVY
                     PGENLNLHIFEPRYKQLISECKKLNKPFGIPTVIENKVQDYGSLVEIIEISKVHDSGE
                     MDIKTKGSRIFRILEVIKEIPEKLYSGAIVTYPENYIQGNPDVMRKVMNSIRDLHKLL
                     KVTKEFKKEDIEMNSYDVAHHIGLSLEEEYELLGLLDERQRQEYLKRHLTKVIPMVAG
                     MEQLKEKIKLNGHFKNLSGFDFS"
BASE COUNT          573 a          289 c          408 g          492 t
ORIGIN      
        1 gtaaatcatt ggcttccctg gtggatgctg aatgctgcag cggcgttcag ttttatgttt
       61 gatattggac tggaagtgca gtttggtgtg ttcatttatg cctttactgt tttatggtgg
      121 tggaaatgga tgaaagagga aaagatataa tctttgtgac ttatgcaatt attgtctgta
      181 gttattgttt gtaaaaatga agccgggatt attggcacta cactgcaaag cctacagggc
      241 ctgacagatg atatcgtagt atatgataat ggcagtacag atgacaccct tgtcattgct
      301 caacaatatg caacgaatgt aataaggggt gagtgggaag gttttggcaa aacaaaacaa
      361 aaagctgtgg ctcttgcaaa gcatgactgg atattgagcc ttgatgctga tgaatctatt
      421 gacgatgagt tgaaaaatga attattgcgc ctcccgttga atgacgaaaa tgttgtgtat
      481 gaaataaagt tcaagaattt ttttggcaac caatggctta aatacggcga atggggaaat
      541 gacagtcata cccgtttatt taaccgtcag aaagtaaact ggaatgaggc accggtgcat
      601 gaaggattgg tattacccgg aggcattcag aaaaaaaggc tgaagggcta tgtgctccac
      661 tatacggcta ctgatattca gagatataaa accannnnnn nnnnnngatg aagtatgcag
      721 cccttaatgc agagaagtat tttgagcagg gaaaaaagat tggctggttc aaaaaatata
      781 tatcagcatt tttctccttt acacagaact atattttccg ggctggattt ttagatggaa
      841 aagccgggta tcgttgtgca atgcttacgg ctacctatac ttttaataaa tacaagaacc
      901 tggaacagtt gataagggct gccacagaaa cactgaaaca ttgaaaaaat ctgtgattct
      961 gtgtttcagt ggctattctt tattaagcac tatttttctc ttattcccta atttgcagct
     1021 aaaagcgttg accaatttca ttcccatatt cccccttggc attgtggtat atcccggtga
     1081 aaacctgaac ctgcacatct ttgagccccg gtacaagcaa ctgatcagcg aatgcaaaaa
     1141 gttgaataaa ccatttggca taccgacggt gattgaaaac aaagtacagg attatggatc
     1201 actggtggaa attatcgaaa taagcaaagt acatgatagt ggtgaaatgg acattaaaac
     1261 aaagggcagc cgtatttttc gcatactgga agtgataaag gagataccgg agaagttata
     1321 tagcggagct atcgtaacct acccggaaaa ttatatacag ggcaacccgg atgtgatgcg
     1381 gaaagtgatg aacagcatcc gtgacctgca taaactgctt aaagtaacca aggagttcaa
     1441 aaaggaagat atagaaatga acagttatga tgtggctcat catataggct tgtcgctgga
     1501 agaagaatat gaactgctgg gcctgctgga tgagaggcaa aggcaggaat acctgaagcg
     1561 ccacttaacc aaagtaatac cgatggtggc gggaatggag caattaaaag agaaaataaa
     1621 actcaacggc cactttaaga atttgagtgg gtttgatttc agttaaaaaa agtatgaaac
     1681 gcttattagt cgggattttt ttaattatgt tttcttttgc ctgcttaagc cagcaaaaga
     1741 ttaatataga ttcattgttc gccactatta ataa
//
LOCUS       DAORLS010000465         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_35182_length_1750_cov_2.545723, whole genome shotgun sequence.
ACCESSION   DAORLS010000465 DAORLS010000000
VERSION     DAORLS010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35182_length_1750_cov_2.545723"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1169)
                     /locus_tag="PK977_20020"
     CDS             complement(9..1169)
                     /locus_tag="PK977_20020"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20482.1"
                     /translation="MKTDEAKRLLKPYIVWVLAPHLESDDPNIQYYYDFSQSIKEYTR
                     VFEELETEWKWQPVTMLDFKTVIHSIAKDKSGKTPLVLNLCDGDEINGTPGVSVIHEL
                     EKHGLIYTGSDAHFYTITTSKIPMKKAFDKAGVPTAGWRVITEKKGSVKGLCKRIGTP
                     LIIKPAVSGGSMGVSVKNVVNTEEELVQRVEEISKGYRGWNLLADGIFAEQFISGPEF
                     TTFITGSADDPDNCMVYEPVERVFHSSLPESEKFLSFDRLWEIYEDESPMPENQNFYE
                     YRSVNGELVPALKKLSFDAYKACGGKGYTRIDIRQDAATGKLYMLEVNAQCGLSEDEN
                     YTSIGAILKASGVSFTEVIGEILKDALRRSVLSQAPVKNKRTAKNKTIIKVK"
     gene            complement(1173..1682)
                     /locus_tag="PK977_20025"
     CDS             complement(1173..1682)
                     /locus_tag="PK977_20025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRF20483.1"
                     /translation="MEPANSPAYRIVSTHEIAEVRQQISNGQNGLFTLTSYQPGEVIA
                     DFSAGTISAEPTYLTVQIDSGKHITLQPDFLQYINHSCEPNVFFNTTTMKLVALKELN
                     PQDELVFFYPSTEWKMNQPFDCYCGHACCQGEIRGGAYLSKEAREKYQLNDFIQQQLA
                     KQDARKKVA"
BASE COUNT          460 a          395 c          356 g          539 t
ORIGIN      
        1 cgggaacact atttcacctt aataatagtt ttgttttttg ctgtccgttt attctttaca
       61 ggtgcttgtg aaagcacact tcttcttaat gcatccttta atatctcccc aatgacttca
      121 gtaaaagaaa caccggaagc ctttaagatg gcaccaatag atgtataatt ttcgtcttca
      181 ctcagtccgc attgtgcatt tacttccagc atatatagtt tgcccgtggc agcatcctgc
      241 cggatatcaa tacgggtata accttttccg ccgcaggctt tgtaagcatc aaagctgagt
      301 ttttttaatg ccggcactaa ttcgccattt acggacctgt attcataaaa gttctggttt
      361 tccggcatgg gcgattcgtc ttcataaatt tcccaaagcc tgtcgaatga cagaaatttc
      421 tctgattccg gcaaagaaga atgaaacact ctttctactg gttcatatac catacaatta
      481 tccgggtcat cagcagaacc tgtgatgaaa gtggtaaact ccggtccgct gataaattgt
      541 tccgcaaaaa tgccatcagc caacaggttc cagccgcggt agccttttga aatttcttct
      601 actcgttgaa ccaactcttc ctccgtattc acaacatttt ttaccgatac acccatgctg
      661 ccccctgaaa ccgccggctt aataataagc ggcgttccga ttcgcttaca aagccctttc
      721 acggagcctt tcttttcagt gatcaccctc catcccgctg ttggcacccc cgctttgtca
      781 aaagccttct tcatcggaat cttggaggtg gtgattgtat aaaaatgagc atcagatcct
      841 gtatagatca ggccatgctt ttctaattca tgtatgacag aaacaccggg ggtcccattg
      901 atctcatcgc catcacacag gttgagaaca agcggtgttt taccgctttt gtcttttgcg
      961 attgaatgaa tgacggtctt aaaatcaagc atggttaccg gttgccactt ccactcggtt
     1021 tccagttctt cgaatacccg ggtatattct ttgatacttt gtgaaaaatc ataatagtac
     1081 tggatgttag gatcatccga ctcaaggtgc ggagccagca cccagacgat gtacggcttt
     1141 agtaatcttt tcgcttcatc ggttttcatt cgtcaggcta cttttttcct ggcatcttgc
     1201 tttgccagct gttgttgaat aaaatcatta agctgatatt tttctcttgc ttctttagat
     1261 aagtaagcgc caccgcggat ctcaccctgg caacaggcat ggccgcaata gcaatcaaat
     1321 ggctggttca ttttccattc ggtagaagga taaaagaaaa caagttcgtc ctgcgggttt
     1381 aattctttta acgctaccag tttcatggta gtggtattga aaaaaacgtt gggctcacaa
     1441 ctatgattga tatactgcag aaaatccggt tgcagggtaa tgtgtttgcc cgaatcaatc
     1501 tgcacagtga gataagtcgg ttcggcagag atagtacctg cagaaaaatc tgcaataact
     1561 tctccgggtt gataagaggt tagtgtaaac aaaccgtttt gtccgttgga aatttgctgg
     1621 cgcacttcag caatttcatg tgtgcttaca atgcggtaag cagggctgtt ggctggctcc
     1681 ataaaagggt taagtaagta aaatgaagtt atagataaac ttcaatgtga caagtttaat
     1741 gctgttaaat
//
LOCUS       DAORLS010000466         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_35452_length_1739_cov_1.873515, whole genome shotgun sequence.
ACCESSION   DAORLS010000466 DAORLS010000000
VERSION     DAORLS010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35452_length_1739_cov_1.873515"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PK977_20030"
     CDS             complement(<1..914)
                     /locus_tag="PK977_20030"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRF20484.1"
                     /translation="MGINPQHQSIIIEGVKKTLGQKESGNLSRYVQAKEAWKIRKTFN
                     REVKSAFLMILGIMSAGFGLKGFLIPNKLVDGGTMGISLLTNRETDLSLSLLIVLINL
                     PFILIAWQQIGKIFSIKSIIAISLLALVVAIFPYPVITQDKLLIAVFGGFFLGAGIGL
                     SIRGGGVIDGTEVMAIFLSRKNSLSVGDFILIFNIIIFSVAAYLIDMETALYSVLIYM
                     SASKTVDFVLDGIEEFTGVTIISPHSDEIAHFIKDKMGRGLTVYSGKRGFGKRGESYT
                     NTDIIFTVVTRLEISRLTTEVEKIDPHA"
     gene            1027..>1739
                     /locus_tag="PK977_20035"
     CDS             1027..>1739
                     /locus_tag="PK977_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRF20485.1"
                     /translation="MTISAVVDVLESLAHPSLQEHYDNAGLITGQASWKCKGIICALD
                     VTEEVVKEAVQKKCNLIVAHHPIVFSGLKKINGKNYVEKTIITAIKNDIAIYAIHTNL
                     DNVIEGVNGKIAIMLGLKNISLLAEKESTLKKLVTFAPGNNADAVRDAIFAAGGGQIG
                     NYSECSFNTEGTGTFKPGSAANPKIGQRGKRHKEQEVKIEVIFPVWLESQLVSAMKAA
                     HTYEEVAYDIFSLSNKYQG"
BASE COUNT          540 a          362 c          368 g          469 t
ORIGIN      
        1 aaggcatgcg ggtcaatttt ttccacttca gttgtcaacc ggcttatttc cagccgggtt
       61 acgacagtaa aaatgatgtc tgtgttggta tagctttcac ctcttttgcc aaagcctctt
      121 ttaccggaat aaacggtcag gcctcttccc attttatctt tgataaagtg ggcaatttca
      181 tcactgtgcg gagaaataat agtgaccccg gtaaactctt caataccgtc cagtacaaag
      241 tctactgttt tggaagcaga catgtagatc aatacagagt aaagggcagt ttccatatca
      301 atcaggtatg ccgcaactga aaagatgata atattaaaaa tgaggataaa atcacctaca
      361 ctaagactgt ttttccggct taagaagatg gccattacct cagttccgtc aattacaccc
      421 ccaccccgga tgctaaggcc gattccggca cctaaaaaga atccgccaaa gacagcgatc
      481 agtaatttgt cttgagtgat gacggggtaa ggaaatatgg ctactaccaa agccagcagg
      541 cttatggcaa tgatactttt gatgctgaat attttgccga tctgctgcca ggctattagt
      601 ataaatggca aattgatcaa aacgatcaaa agggagagag agaggtcggt ctcccggttg
      661 gtcagcagcg aaattcccat tgtgcctccg tcaaccagtt tattgggaat caggaatccc
      721 ttcaatccaa aacctgcaga catgattcca aggatcatta gaaaagcaga cttgacttcc
      781 ctgttaaatg ttttcctgat cttccaggct tccttggctt gtacgtaacg agacaggtta
      841 ccgctttctt tttgaccaag tgttttcttc actccttcaa taataatact ctggtgctgc
      901 ggatttattc ccatagtttt aaagataaca aaaagaggct ttttagaagc agccaaccag
      961 tcaaatactt aactttccgt cataaacatt taatccatat aattataaat agccaaaagg
     1021 ttgaatatga caatatctgc tgttgttgat gtactggaat cgcttgcgca tccctccttg
     1081 caagaacatt atgataatgc agggctcatt accggtcagg cgtcctggaa gtgtaaagga
     1141 ataatttgtg cactcgatgt taccgaagaa gtggtaaaag aagctgtgca gaaaaaatgt
     1201 aatctcatag ttgcacatca tcccatagtg ttcagcgggt taaaaaaaat taatggaaaa
     1261 aattatgttg aaaaaaccat tataaccgct ataaaaaatg atattgctat ttacgccata
     1321 cataccaacc tggataatgt gatcgaggga gtaaacggga aaattgcaat catgctgggt
     1381 ttaaaaaata tttccctgtt ggctgaaaaa gaaagcacac ttaaaaaact ggtaacattt
     1441 gctcctggaa acaatgcgga tgcggtgcgg gatgccatat ttgctgccgg cggaggccag
     1501 attggtaact atagcgaatg cagtttcaat acggaaggaa ccggcacctt taagccggga
     1561 tcagccgcca atcccaaaat cggtcaaaga ggaaagcgtc ataaagagca ggaagtaaag
     1621 atcgaagtca tctttccggt ctggcttgaa agccagctgg tttcggctat gaaggctgca
     1681 catacctatg aagaggtggc ctacgacatt ttttctcttt ccaacaaata ccagggctt
//
LOCUS       DAORLS010000467         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_35660_length_1732_cov_1.970185, whole genome shotgun sequence.
ACCESSION   DAORLS010000467 DAORLS010000000
VERSION     DAORLS010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35660_length_1732_cov_1.970185"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PK977_20040"
     CDS             <1..699
                     /locus_tag="PK977_20040"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF20486.1"
                     /translation="IQQDKAAMKVTTDSCLFGAWMAKKMTEHTGTPSRLLDIGIGTGL
                     LSLMIAQQNRHCTIDAVEIDRNAYEQAIENVMSSPWKENIQVFHADIKSIEPGKKYDV
                     IISNPPFYENELKAENENKNFAHHNSGLAFSDLFEIIKRQLSPEGHFYLLLPYKRNEE
                     IRKLVFEHRFELSQIVLVRQSVNHSYFRIMLCGKLSSEEREKTVIDEMAIRDSNYNYT
                     PAFISLLRDYYLYL"
     gene            complement(694..>956)
                     /locus_tag="PK977_20045"
     CDS             complement(694..>956)
                     /locus_tag="PK977_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRF20487.1"
                     /translation="VYGVDRKSLKKTAPYLPDSIDVMAVGNLPNELPRDASRYFGEQL
                     IKYILEDLIGDSSEIIERATIAHSGKLTPYFSYLQDYADGKL"
     assembly_gap    957..1015
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1016..>1732)
                     /locus_tag="PK977_20050"
     CDS             complement(<1016..>1732)
                     /locus_tag="PK977_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRF20488.1"
                     /translation="VEVKEDISDCDILLGIKEVPAAELIEGKTYLFFSHTKKKQPHNQ
                     PLLRAILDKRINLIDYECLEHEDGQRIIGFGFFAGVVGAHNGMMAYGSRTGLFKLDRV
                     YKQRSFRELIHNYFGLKLPNVKIAVTGSGRVAHGILEIMNLMGIHEVEPDEYLKRRFA
                     YPVYCQFKGSELYEHKETRKYSREDFHENPENYNCKFLPYAAQTDILMNGVYWDKNVP
                     RLFEKADIKSDTFIIQTIAD"
BASE COUNT          521 a          355 c          313 g          484 t
ORIGIN      
        1 atacagcagg acaaggctgc catgaaggtt accacagata gttgcctctt tggagcctgg
       61 atggcaaaaa agatgacaga acataccgga acaccatcac ggttattgga tattggtatc
      121 gggactggtt tgctaagcct gatgatagcc cagcaaaatc gtcattgtac aattgacgct
      181 gttgaaattg acagaaacgc ttatgaacag gctattgaga atgtaatgag ttccccctgg
      241 aaagaaaata tacaggtttt tcatgcagac ataaaatcta ttgaacccgg gaaaaagtat
      301 gatgtcatca taagcaatcc tccattttat gaaaatgagc taaaagcaga aaacgagaat
      361 aagaattttg cccatcacaa ttcagggctt gcattcagtg acttatttga aattattaaa
      421 cggcagctta gcccggaagg gcatttttat ctgctgctgc catacaaaag aaatgaagag
      481 atcaggaaac tggtttttga acaccgattt gaattaagcc aaatagttct ggtaagacag
      541 tctgtcaatc atagctattt ccgtatcatg ctttgcggaa aattatcatc agaagaaaga
      601 gaaaaaaccg tcattgacga aatggcgata agagactcca attataatta tacacctgcc
      661 tttatttcac tgctgagaga ctactacttg tatctataat ttaccatcag cataatcctg
      721 caaatagctg aaatatggag ttagctttcc cgaatgtgct atagtggctc tttcaataat
      781 ctcagaactg tctccaataa gatcttccag tatgtatttg atcagttgtt caccaaaata
      841 ccggctggca tctcttggca attcattggg caggttaccg acggccatca catcaattga
      901 gtctggtaaa taaggggctg tttttttcag gctttttctg tcaaccccat aaaccgnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatatc
     1021 agcgattgtt tgaatgataa acgtatcgga ttttatatct gctttttcaa aaagccgggg
     1081 gacattttta tcccaataaa ctccattcat taaaatatcg gtctgggcag catagggcag
     1141 aaatttgcaa ttatagtttt ccggattctc atgaaaatct tcacggctgt atttacgggt
     1201 ttctttatgc tcatacaatt cagatccctt aaactggcaa taaaccgggt aggcaaatct
     1261 cctttttaaa tactcatccg gctctacttc atgaataccc atcaggttca taatctccaa
     1321 aatgccatga gccacacgac cggaaccagt aactgctatc ttgacatttg gaagtttaag
     1381 tccgaaataa ttatgaatca attccctgaa acttcgttgc ttatataccc tatctaattt
     1441 aaataaaccg gtccggctac catatgccat catgccgttg tgtgcaccaa ctacgccggc
     1501 aaaaaagcca aaaccaatta tccgctggcc atcctcatgc tccaggcact cataatctat
     1561 cagattaatt cttttgtcga gtattgccct cagcaaaggc tggttgtgag gctgcttttt
     1621 cttggtgtga gaaaagaaaa gataagtttt gccttcaatc aactcagcag caggcacttc
     1681 cttaatgccc agtagaatat cacaatcaga aatgtcctcc tttacctcaa ct
//
LOCUS       DAORLS010000468         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_36913_length_1681_cov_2.163592, whole genome shotgun sequence.
ACCESSION   DAORLS010000468 DAORLS010000000
VERSION     DAORLS010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36913_length_1681_cov_2.163592"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PK977_20055"
     CDS             <1..618
                     /locus_tag="PK977_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20489.1"
                     /translation="LLVVFAITLAIQPDIYTQLIDTIFYSSKADENEHHIPNYVIGLS
                     ILGLVILITGIWMIRNKKSFKDLGLVFKRIGQSIWQGLSAVQHLKRRTRFIILTLILW
                     GLYLTAGYIGFFALQQTSQYGIREAFTILSAGSIGMIATPGGIGAYAYLIQKTMQVYG
                     LNEGIALAFGWILWLAQTAVILVGGLISFVAIPIYNKKKKTVETA"
     gene            605..1096
                     /gene="rfaE2"
                     /locus_tag="PK977_20060"
     CDS             605..1096
                     /gene="rfaE2"
                     /locus_tag="PK977_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRF20490.1"
                     /translation="MKRPDLIPKKILTREEAVQRVAQWRLHGKTVAFTNGVFDILHQG
                     HIFSLSQAAKEADYLIVGLNADISVKRLKGENRPVNNQESRGLVLSSLIMVDAVVLFE
                     EDTPLDLITSLLPDVLVKGGDYTVEQIAGAKEVIASGGRVVINPILEGFSTTGIIAQL
                     KSN"
     gene            1171..>1434
                     /locus_tag="PK977_20065"
     CDS             1171..>1434
                     /locus_tag="PK977_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRF20491.1"
                     /translation="MAQPGIKRKTIARDISWLSFNARVLQEAADPTVPLRERIRFLGI
                     FSNNLDEFFRVRVATLRRMIQFGSKANMHLENNPQQIIDEIQMI"
     assembly_gap    1435..1453
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1454..>1681
                     /locus_tag="PK977_20070"
     CDS             <1454..>1681
                     /locus_tag="PK977_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRF20492.1"
                     /translation="NRIWEEVMKELNLQKIYLRTEKELNAEQQQFVSNYFEEEVSSDV
                     IPLMIENITEFPALRDKSIYLGVVMWKKESAL"
BASE COUNT          512 a          335 c          354 g          461 t
ORIGIN      
        1 ctcctggttg tttttgctat tacactggct attcaaccag atatttatac tcaactaata
       61 gacaccattt tctactcctc aaaagcagac gaaaatgaac atcacatacc caattatgtt
      121 atcgggcttt ccatacttgg acttgttatt ctaataacgg gtatctggat gataagaaac
      181 aagaagagtt tcaaagacct tggcctcgtc ttcaaacgta ttgggcaaag tatctggcag
      241 gggcttagtg ctgtacagca cctgaaaagg agaacaaggt ttattattct tacacttatt
      301 ctctggggac tatacctaac ggcaggctat atcggctttt ttgccctgca gcaaacctct
      361 caatatggca tccgggaagc ttttaccatc ttatcagccg gaagtattgg catgatcgca
      421 acacccggcg gtattggtgc ttatgcatac cttattcaaa aaaccatgca ggtgtacgga
      481 ttaaatgaag gtattgccct tgcatttggg tggatactgt ggctggcaca aacagccgtg
      541 atccttgtcg gcggtttaat tagctttgta gctataccta tttacaacaa aaagaaaaaa
      601 actgttgaaa cggcctgatc ttataccgaa aaaaatactt acaagggaag aggctgttca
      661 acgagtggct caatggagac ttcatggcaa aactgttgct ttcactaacg gtgtttttga
      721 tattctgcac cagggtcata ttttttcttt gtcacaggca gcaaaagaag cagattatct
      781 tatagttggt ttaaatgctg atatatcggt aaaacggtta aaaggagaaa accgtccagt
      841 caataaccag gaatccaggg gcctggtact atcttcatta atcatggttg atgcagtggt
      901 attgtttgaa gaagataccc cattagatct gatcacttca ctattaccgg atgtattagt
      961 gaaaggcggc gattataccg tggagcaaat cgccggcgcc aaagaagtta tagctagtgg
     1021 cggaagggtt gtcattaatc ctatactcga aggattttcc actaccggca tcatcgctca
     1081 gctcaaatct aactgagccg caaacctgta aacttaatac cgcggttagc actcctttgt
     1141 taactttact acaatcagaa tcattaacca atggcacaac cgggaatcaa aagaaaaact
     1201 attgcaagag atatcagctg gctatcattc aatgcaagag tattacagga agcagctgat
     1261 cctactgtac cactgagaga acggatacgc ttccttggta ttttctccaa taacctggat
     1321 gagtttttcc gggtaagggt agctacgctt cgtcgtatga tacaatttgg cagcaaagcc
     1381 aacatgcact tggagaacaa tccccagcaa ataattgatg aaatacagat gatcnnnnnn
     1441 nnnnnnnnnn nnncaaccgt atctgggagg aggtaatgaa agagctcaat ctgcagaaga
     1501 tctacctgcg gactgaaaaa gaactgaacg cagaacaaca gcagtttgtc agtaattatt
     1561 ttgaagaaga agtaagttca gatgttatac cattaatgat tgagaatata acagagttcc
     1621 ctgctttgcg tgataaatct atttacctcg gtgtggttat gtggaaaaaa gagagtgccc
     1681 t
//
LOCUS       DAORLS010000469         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_37127_length_1672_cov_1.717378, whole genome shotgun sequence.
ACCESSION   DAORLS010000469 DAORLS010000000
VERSION     DAORLS010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37127_length_1672_cov_1.717378"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..500
                     /locus_tag="PK977_20075"
     CDS             147..500
                     /locus_tag="PK977_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20493.1"
                     /translation="MKKVSLLTLIITGVFVLSNCNPAQKVTTSAPAKLTYEANLMTVL
                     ANNCSPCHIPAKGGKKKPYDTYASVKTDIDEIIRRIELNPADKGFMPFKKTAKLSDST
                     ILVFKQWKADGLLEK"
     gene            complement(507..968)
                     /locus_tag="PK977_20080"
     CDS             complement(507..968)
                     /locus_tag="PK977_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20494.1"
                     /translation="MTRLYLFLLLFLPAISFGQRYMNFTKSALKDSLAAKNEKKKGVT
                     TSLKDTKDELIYTIKGPGTDQTDIIYSFDSLGRCLREKVIAGCDTCIHSKLQSILNIQ
                     SYKWKKINENQYVSRFEDRLMIELSVNKQEHSFYIHRMEWTQLLYDILLGK"
     gene            1085..1648
                     /locus_tag="PK977_20085"
     CDS             1085..1648
                     /locus_tag="PK977_20085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF20495.1"
                     /translation="MKKISSLLLVGFALVLQNCDKDDEPDNRPVARFTPSGYNQPVPC
                     TINFINNSSNATSYSWSLGDGTTTTNPNPTRTYTAIGTYYVTLKVTGPNGSDSVCRSI
                     TLDQVIPNESTFSYYMDKCSGTPVSVQFYSLNPQSQFHAWDFGNGVTTPEANPLMRFE
                     TPGNYTIKYSSQIGGIRDTIIRAIQIN"
BASE COUNT          505 a          328 c          330 g          509 t
ORIGIN      
        1 ttgctgtagc cgatatcatg ggtgcggcaa aggtacagtt tggtggtttg gttttggagc
       61 ttgtggcaac ctgttgttgt taattacacg attggaaatg tattgttcag gatattttat
      121 acatttattc caaacactta accaacatga aaaaagtatc tttattaaca ttgatcatta
      181 caggagtttt cgttttatca aattgtaacc ctgcccaaaa agtcacaaca tctgcaccag
      241 caaaacttac ctatgaggca aaccttatga cagtattagc caacaattgc tccccctgcc
      301 atattcctgc aaaaggcggt aagaagaagc cttatgatac atatgccagt gtgaaaactg
      361 acattgatga gatcattcgc cgaattgaac tgaatcccgc agataaggga tttatgcctt
      421 tcaaaaagac ggctaaactt agcgatagta ctattttagt tttcaaacag tggaaagcag
      481 acgggcttct tgaaaaatag ctatacttat ttacccagca gaatatcata tagcagctgg
      541 gtccattcca tacggtggat ataaaatgag tgctcctgtt tgttaacaga aagctcaatc
      601 atcaggcggt cctcaaagcg ggaaacatat tgattttcgt ttatcttttt ccacttatag
      661 gattggatat tgagaataga ttgcagtttt gaatgaatgc aggtatcgca tccggctata
      721 actttttccc tgagacatct gccaagactg tcaaagctgt atataatgtc tgtttgatca
      781 gtgcccggtc ccttaatggt gtagatcagc tcatcctttg tgtcttttaa gctggttgta
      841 accccttttt tcttttcatt tttagctgca agagaatcct ttaaggctga tttggtaaaa
      901 ttcatatatc gttgcccaaa ggaaattgca ggcagaaaaa gcagcagaaa taaatataaa
      961 cgggtcatat atcgttataa tttgagtgtg tcaaaattag gttctttctg tcttcagaac
     1021 ttttgccggc aggcttaaaa gatgaaaatc atgtactttt gaattatcaa aaatgtatag
     1081 ggttatgaaa aaaatttctt cattgttatt ggttggtttt gcgctggtgt tgcaaaactg
     1141 tgataaggat gatgagccgg ataaccggcc ggttgcaagg tttacccctt ccgggtataa
     1201 tcaaccggta ccctgtacga tcaactttat taataattcg tctaatgcca caagttattc
     1261 ctggagcctg ggcgatggca caaccacgac taatcccaat cccacaagaa cctacacagc
     1321 tataggtacc tattatgtga cattaaaagt tacagggccc aacggttcag attctgtttg
     1381 ccgttccata acactcgacc aggtcattcc taatgagtct acattttctt attacatgga
     1441 caaatgttct ggaactccgg taagtgtaca gttttattca ttgaacccgc aatctcagtt
     1501 tcatgcctgg gattttggga acggagtaac cactcctgaa gcaaaccccc tgatgcgatt
     1561 tgaaacacca ggtaattaca ctatcaaata cagttcacag attggcggga tacgggatac
     1621 tattataagg gctattcaga taaactaacc taataaaaac agaaaagcct ca
//
LOCUS       DAORLS010000470         1655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_37562_length_1655_cov_0.960000, whole genome shotgun sequence.
ACCESSION   DAORLS010000470 DAORLS010000000
VERSION     DAORLS010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37562_length_1655_cov_0.960000"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    219..412
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <413..>1182
                     /locus_tag="PK977_20090"
     CDS             <413..>1182
                     /locus_tag="PK977_20090"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRF20496.1"
                     /translation="FTIFLLITSWGTFAFAITRITQFVVSGEINQYFKTRKLMKDISH
                     LHNHVILCGYGRNGHQAARILEAHQVPFVVIEKDEHLMQKMVADGEKILHLEGDSTDD
                     ELLRLAGIERAKALITTLPVDAQNVFIVLSARALNPKIQIISRASEASSVSKLKKAGA
                     DNVIMPDHIGGTHMATLISKPDVVEFIDFLSGEEGHSIYMESVSFDHLPSVLKGKPLR
                     DVMQWNKTGVNCIGIKDAEGRFIINPPEETTITPGMKV"
     assembly_gap    1183..1378
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1379..>1655)
                     /locus_tag="PK977_20095"
     CDS             complement(<1379..>1655)
                     /locus_tag="PK977_20095"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF20497.1"
                     /translation="PSSVVFDAFTNPLSLQKWWGVERCLVEHKQGGLYSLAWNISKKG
                     IQYISTGVITVFIPGKELLIDHLVYFNPDKPILGPAYLDIKFREGNG"
BASE COUNT          369 a          261 c          298 g          337 t
ORIGIN      
        1 aagcagcgga ttgaatattg tagtgttaaa agacatggca gcagaacagg caggtatgta
       61 tgtacttgaa atgattactg aggatgaaag gctggttcag aaactgatca ggaaataggg
      121 acggcaaccg gcttttcggg caggattgat tcattcaatc ctgctttttt ttgctcattg
      181 ggtatctgca gtctattaat tatttttatc acaaatcann nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttcacca
      421 ttttcctgct cataaccagc tggggcactt ttgcatttgc cattacccgg ataacccagt
      481 ttgtggtaag cggggagatc aatcaatact tcaaaaccag gaaactcatg aaagatatta
      541 gtcatttgca caaccatgtc atcttatgcg ggtatggcag aaacggacac caggcggccc
      601 gcatactgga agcccaccag gttccttttg tagtgattga aaaggacgaa cacctgatgc
      661 agaaaatggt ggccgatggc gaaaagatcc ttcacctgga aggcgacagt acagacgatg
      721 aactgctcag gctggccggt attgaacggg caaaggcatt aataaccact ttgccggtgg
      781 atgcacagaa tgtatttatt gttttatcgg cccgggcatt aaatccaaaa attcagatca
      841 tcagccgggc ttccgaagcc agctctgtta gtaaattaaa aaaggccggt gcagataatg
      901 ttatcatgcc tgatcatata gggggcaccc atatggctac acttatttca aagccggatg
      961 tagtggagtt tattgatttt ctttccggcg aagaaggcca ttccatctat atggaatccg
     1021 tttcatttga ccacctgccg tcggtattga aaggcaaacc cctcagggat gtgatgcagt
     1081 ggaataaaac aggtgtgaat tgtatcggga tcaaagatgc tgaaggaagg ttcattatta
     1141 acccgccgga agaaacaacc ataacaccgg gaatgaaagt aannnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     1381 accgtttcct tcccggaatt taatgtcaag ataagctggt cccagtatgg gtttatccgg
     1441 gttgaagtaa acgagatgat caatgagcaa ttctttgccg ggaataaata cagttattac
     1501 accggtagaa atgtattgta ttcctttctt actgatgttc catgccaaag aatagaggcc
     1561 gccctgtttg tgctccacca ggcatctttc cactccccac cacttttgca atgataaagg
     1621 gttggtgaat gcatcaaaaa ctactgatga cggct
//
LOCUS       DAORLS010000471         1637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_38041_length_1637_cov_1.680152, whole genome shotgun sequence.
ACCESSION   DAORLS010000471 DAORLS010000000
VERSION     DAORLS010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38041_length_1637_cov_1.680152"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1307)
                     /locus_tag="PK977_20100"
     CDS             complement(90..1307)
                     /locus_tag="PK977_20100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRF20498.1"
                     /translation="MPGYELWSDKERKEVNDVLETGILMRYGFDGPRKGVWKSKKLES
                     AINETFGCKYAQLTSSGTAALTTAMSALGIGYGDEVITPSFTFVASFEAVLSVGAVPV
                     LVDVDDTLTLDPEAVRKSITPKTKAIMPVHMCGSMADLDVLVAICKENNLILLEDACQ
                     SIGGTYKGKHLGTIGDAGTFSFDFVKTMTCAEGGVVMTNREDIYVNSDGYTDHGHDHK
                     GADRGADLHPFIGYNFRISELHAAVGLAQIKRLPEFLTIQKKNHGLLKNILAQVPEVS
                     FRRIPDPAGDSCSFVSWFLPTEEITRAVVAEMKAQGILPGNFYWYDNNWHYIRKWDHL
                     KNAVTLNALGPELKEKVMQQANKDFAASDAVMSRCISTSISLLWTEEQIQEKGEKMVN
                     AVKKVLSTQPVSA"
BASE COUNT          455 a          382 c          305 g          495 t
ORIGIN      
        1 ttctctcact tttgaaaaaa tcagaaaata gtttggtagc tatcattgta aatggatagt
       61 tagttattac ataatgtgct tttgaaaact caggcagaaa caggctgggt gctcagtact
      121 ttctttacag cattcaccat cttctcccct ttctcctgta tttgttcttc tgtccacaat
      181 aaactgatgg aagtagaaat acaacgactc attacagcat cactggcagc aaagtctttg
      241 ttcgcctgct gcattacttt ttctttcagt tcaggaccta aggcatttaa tgtcaccgca
      301 tttttcaggt gatcccattt gcggatataa tgccaattat tatcgtacca gtaaaaatta
      361 ccgggcaaaa ttccctgtgc tttcatttcg gccaccacgg ccctcgttat ttcttcagtc
      421 ggtaagaacc agctaacgaa actgcaacta tctccggcag gatctggtat acggcggaaa
      481 gaaacctccg gcacctgtgc taatatattt ttcagcagtc cgtgattttt tttctgaatg
      541 gttaaaaatt caggcagtct ttttatttgt gctaacccca ctgctgcatg cagttcagaa
      601 atgcggaaat tataaccgat aaacggatgc aggtcagcac cacggtccgc tcctttgtga
      661 tcatgaccgt gatcggtata gccatcactg tttacataaa tatcctcccg gttagtcatc
      721 accacaccgc cttcggcaca ggtcatggtt tttacaaaat caaatgaaaa agtaccggca
      781 tcaccaatgg tgccaagatg cttgccttta taggtaccac ctatgctctg gcaggcatct
      841 tccagcagta ttaaattgtt ctctttacaa atagctacca ggacatccag atcggccata
      901 ctaccacaca tatgcaccgg catgatagct tttgttttag gtgttatcga tttgcgtacg
      961 gcttcgggat ccagggtgag tgtatcatcc acatctacca gcaccggcac agcgccaacg
     1021 cttaacactg cttcaaaact tgctacgaag gtgaaagagg gggttatcac ttcgtcacca
     1081 taaccaatac ccaatgcact catggctgtg gttaatgcag ccgtaccact ggaagtaagc
     1141 tgggcatatt tacaaccaaa tgtttcgttg atggccgatt ccaacttttt gcttttccag
     1201 acacctttcc ggggtccatc aaagccataa cgcatcagga tgcctgtttc cagcacatca
     1261 tttacttcct ttctttcttt atcactccag agttcgtagc cgggcatatt cgttatttaa
     1321 agtttgaagc ttactgcttt aagttgttta agcacagcct tgcaaaggta actattgcca
     1381 aataaagaga atttaagaat actttacact tagcacacat acacaaagta ttgtagcaat
     1441 gtaccttgta tttaggcttt tacaaatgag aaaactgctt attatagtac tatcacattt
     1501 cccattaata actattgctc aagtaaaaac cggtgtaaga ggtggaataa atcttgccca
     1561 atttgtttac agacctgcct atgatggcga cacaaaagga aaagggtctt tactattccg
     1621 ttttaatgca ggccttc
//
LOCUS       DAORLS010000472         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_38432_length_1623_cov_1.732781, whole genome shotgun sequence.
ACCESSION   DAORLS010000472 DAORLS010000000
VERSION     DAORLS010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38432_length_1623_cov_1.732781"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK977_20105"
     CDS             complement(<1..207)
                     /locus_tag="PK977_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRF20499.1"
                     /translation="MTRIGLISDTHGFLDEAIFKHFEQCDEIWHAGDFGSGVADQLKK
                     FRPLKGVYGNIDDQAIRNTFSEQVV"
     gene            415..1572
                     /locus_tag="PK977_20110"
     CDS             415..1572
                     /locus_tag="PK977_20110"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRF20500.1"
                     /translation="MPHYYKLGTIPHKRHTQFRKPDGGLYSEQLFSTEGFSDDYALLY
                     HCHPPTQIIKTEPQVDVSPQIAEEKMLSHRCFEGFHIKPGGEFLQSRIPVLVNSDCHI
                     VLAAPQSGTKDYFYKNTDADEMIFVHEGSGTVKTQYGQLPFSYGDYIVLPRGTIYQVE
                     FNDDKNRLFIVESFSPLRFPKRYMSKYGQLMEHSPYCERDIRPPQELETFDEKGDFLI
                     KAKKKGVLYGLHYGTHPFDVIGWDGCCYPYIFSIHDFEPITGRVHMPPPIHQTFETNS
                     FVVCSFVPRLYDYHPNAIPAPYNHSNIDSDEVLYYVDGDFMSRKHVTRGMITLRPAGI
                     PHGPHPGAVEKSIGAKETKELAVMVDTFRPLMLTKQALEIENKNYVMSWAE"
BASE COUNT          478 a          349 c          354 g          442 t
ORIGIN      
        1 aacaacttgc tcagaaaagg tgtttcggat ggcctggtcg tcaatattgc cgtatactcc
       61 tttcagcggt ctgaactttt tcaactgatc ggcaacgccg gatccaaaat caccggcatg
      121 ccatatttca tcacattgct caaagtgttt aaagatagct tcatccagga agccatgtgt
      181 atcagatatc agccctattc ttgtcaaacg gaattgtttt acctttatta agcaaattaa
      241 aacaaatgcc ctttcaccgc aatttcacct actacattga taagcgcaaa tggaagtata
      301 tctttctttt ggccaccctt gaactttcaa tttgaaaacc ggcttaaata aaaagaaggt
      361 ttaataagtc ttttgcaatc tgcaatccta aatctaaaat ctaaaattcg aatcatgcct
      421 cattattata aactcggaac cattccgcat aagcgtcaca cccagtttag aaaacctgat
      481 ggtggactat acagcgagca gttattttca acggaaggat tcagcgatga ttatgccttg
      541 ctctaccact gtcatccgcc tacccagatc atcaaaacag aaccgcaggt agatgtaagt
      601 cctcaaattg ctgaggaaaa aatgctcagt caccggtgct tcgaaggatt tcatataaaa
      661 cccggtggtg agttcctgca aagcaggatt ccggtgctgg taaacagtga ctgtcacatt
      721 gtgctggcag cgcctcaaag cggcacgaag gattatttct acaagaacac cgatgccgat
      781 gaaatgatct ttgtacatga aggaagcggt acggtaaaaa cacaatatgg acagttgcct
      841 ttcagctatg gcgattacat cgttttgccc aggggaacta tttaccaggt agaatttaac
      901 gatgataaaa acaggctgtt tattgtcgaa tcattctcgc cgctgcggtt tcccaaaaga
      961 tacatgagca agtacggcca gctgatggag cattcaccct attgtgaaag agatatccgg
     1021 ccgccgcagg agcttgaaac ctttgatgag aaaggagatt tcttaatcaa ggccaaaaag
     1081 aaaggagtgt tgtatggcct gcattatggt actcatccct tcgatgttat tggctgggat
     1141 ggctgttgtt atccatatat cttttccatt catgattttg aacccataac cggaagggtg
     1201 catatgccgc cgcctataca ccagactttt gaaacgaaca gttttgtggt ctgttcattt
     1261 gtaccaaggc tatatgatta tcaccccaat gctatacctg ctccttataa tcacagtaat
     1321 attgacagtg atgaagtatt gtattatgtg gacggggact ttatgagccg caaacatgta
     1381 accaggggca tgataaccct gcgccccgcc ggtattccgc atggtccaca tccgggtgct
     1441 gtagagaaaa gcattggcgc caaagaaaca aaggaactgg cggtaatggt ggatactttt
     1501 cgtccgctga tgctcacaaa gcaggcattg gagattgaaa acaagaatta tgttatgagc
     1561 tgggcggagt agttgtcacc ctgagcctgt cgaagggtga taattgaagg tgtaataaat
     1621 atg
//
LOCUS       DAORLS010000473         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_40207_length_1563_cov_1.603448, whole genome shotgun sequence.
ACCESSION   DAORLS010000473 DAORLS010000000
VERSION     DAORLS010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40207_length_1563_cov_1.603448"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..468)
                     /locus_tag="PK977_20115"
     CDS             complement(31..468)
                     /locus_tag="PK977_20115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF20501.1"
                     /translation="MSIQNKLREFMNGKIEAQKSAGAAFMTQNITREGVKELPGGIQY
                     EVIKEGTGVQPSVTDSVKAHYKGTLLDGKEFDNSFKRGQPFTAPLRALIKGWQVALPM
                     MKEGSQWRLWIPSDLAYGDRGAGNDIPGGATLIFEVELLQVIK"
     gene            complement(471..1232)
                     /locus_tag="PK977_20120"
     CDS             complement(471..1232)
                     /locus_tag="PK977_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF20502.1"
                     /translation="MANTIISVKNLVKNYGNFQAVKGISFDVKEGEIFGLLGPNGAGK
                     STTLEIIETLRPKTSGEIIVDGLCLDKEPNEIKKIIGVQLQTSGYYPGLNLLQLIELF
                     CGLYNRYVDPMELLEMVNLKEKAKAKVKELSGGQKQRFSVATTLINEPRIIFLDEPTT
                     GLDPQARRSLWELIKKIRERGTTVIITTHYMDEAEYLCDRVAIIDAGKIIAMDSPDKL
                     IDDLVAGGFERPKEMKMANLEDVFIHLTGHSLREG"
     gene            complement(1234..>1563)
                     /locus_tag="PK977_20125"
     CDS             complement(1234..>1563)
                     /locus_tag="PK977_20125"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRF20503.1"
                     /translation="EYIATCQKKDKETGGPYTQRYIGSMVSDVHRNLIKGGIFMYPGT
                     TDKPKGKLRLLYECNPFAFIVTVAGGKATNGKIRILDVEPTELHQRSPFFVGSKLMME
                     ELETYLN"
BASE COUNT          396 a          381 c          268 g          518 t
ORIGIN      
        1 tgggtcagtt gcaacagatg gcataaatga tcatttaatt acttgtaaaa gttccacttc
       61 aaaaataagc gttgctccgc cgggtatatc attccctgct cccctgtcgc cgtaggcaag
      121 atcagatggt atccataatc tccattgact tccctctttc atcataggta aagctacctg
      181 ccatcctttg attaatgccc gcaatggtgc agtgaaaggc tgccctcttt taaacgagtt
      241 atcaaactcc tttccatcga gtaaagtacc tttatagtga gcttttacac tatctgttac
      301 tgacggctgc acaccagttc cttctttaat cacttcgtat tgtatcccgc cagggagttc
      361 ttttactcct tccctggtga tattttgggt cataaatgca gctcctgctg atttttgcgc
      421 ttcgatttta ccattcataa actctcttaa tttattttga atactcataa ttaaccctcc
      481 cgcagtgaat gaccggttaa atgaataaaa acgtcttcca ggttagccat tttcatttct
      541 tttggtcttt caaaacctcc ggccaccagg tcatcaatca gtttatccgg agaatccatg
      601 gctataattt tacctgcatc aatgatagct accctgtcac acaggtattc tgcttcatcc
      661 atgtagtgtg tggtaatgat aacagtggtt cctctctccc tgattttttt gatcagttcc
      721 cataagctcc tccttgcctg gggatcaaga ccagtagtgg gttcatccag gaatatgatc
      781 cgaggttcat tgatgagtgt agttgctaca gagaatcttt gtttctgccc gccgctaagc
      841 tctttcactt tcgcctttgc cttttccttc aggttaacca tttccagcaa ttccataggg
      901 tcaacgtacc tgttgtataa cccgcagaac agttctatca actgaagcaa attaagaccg
      961 ggataataac cgctagtttg caactgtacg ccaattatct ttttgatctc attgggttcc
     1021 ttatcaagac acaaaccatc aactattatt tcaccgcttg ttttaggcct taaggtttcg
     1081 attatttcca aagtggtaga cttaccggca ccattaggtc ccagtaaccc aaatatctct
     1141 ccctctttca catcaaaact gatacccttg actgcctgga agttaccata gttttttaca
     1201 aggttcttaa ctgatattat agtatttgcc atattaattc aaatatgttt ccagttcttc
     1261 catcatcagt ttactaccca caaagaaagg actacgctga tgtaattctg tcggctctac
     1321 atccaatatc cgtatttttc cattggtagc tttaccgcct gctacggtaa caataaaagc
     1381 aaacgggtta cattcataca acaaccggag tttgcctttt ggcttatcag tggttccggg
     1441 gtacataaaa attcctccct taataagatt gcggtgcaca tccgaaacca tgcttccgat
     1501 ataacgttgt gtgtacggac ctcccgtttc tttatccttt ttctgacagg tggcaatata
     1561 ttc
//
LOCUS       DAORLS010000474         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_41719_length_1514_cov_1.864976, whole genome shotgun sequence.
ACCESSION   DAORLS010000474 DAORLS010000000
VERSION     DAORLS010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41719_length_1514_cov_1.864976"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1514)
                     /locus_tag="PK977_20130"
     CDS             complement(<1..>1514)
                     /locus_tag="PK977_20130"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF20504.1"
                     /translation="YVAGLNAYAKKYPGKVKYKKAFPFTEKDYMTAVVFSISLFCGVD
                     DALPQILGGKVATIPGFNPQGSNAFAMNSVKTTTGESFLAINAHQPNTGPVAFYEAHL
                     QSEQGWNMLGGLFPGGCLIFHGTNENLGWAHTVNYQDKLDIYQLEMNPANKNQYKFDG
                     QWIDLEVNKAKLKVKGIPVTISKKIYWSKYGATLKTGKGVFSLRVPATMDIKALEQWY
                     RMNKAKNFTEFYKALSMTSLPMFNIMYADRYDTIFYISNGKMPKRNPDPKYNWKSTLP
                     GNTSATLWTEFKSIKELPQYINPPSGYLFNTNHSPFLATDKVYNLDKNKFDRTDGWET
                     YHNNRSQRVTELINSEKTDYTTFKRVKFDQQLPKELKYTYSIDSMLNLNAADFPQLKD
                     VITTFQAWDRKSIASSRGAAVFLLTYYYLADRLKGVPSRKITKAEAAETYQYVYDYML
                     KYFGKTDLTLGDIQKLVRGDEERPAWGFPDVLAPSFSEPYKNGRRKVTGGDAYI"
BASE COUNT          380 a          332 c          314 g          488 t
ORIGIN      
        1 agatataagc atcaccacct gttacttttc gccttccgtt tttataaggc tccgagaagg
       61 aaggcgcaag cacatccgga aaaccccatg ccggtctttc ttcgtcgcct cttaccaatt
      121 tctgaatatc tcctagtgtc aaatcggttt taccaaagta tttcagcatg taatcataaa
      181 catactgata ggtttctgcg gcctcagctt tggtgatctt tctcgaagga acacctttaa
      241 ggcgatctgc gaggtaataa taagtaagca ggaaaacagc agctcctcta ctggaagcaa
      301 ttgattttct atcccatgcc tggaatgtgg ttataacatc tttcaactgc ggaaaatcag
      361 cagcattcag gttgagcata ctgtcaatgc tgtatgtgta ttttaattct tttggcagct
      421 gctgatcaaa ttttacccgt ttaaatgtgg tataatctgt tttttcggag ttaataagtt
      481 cggttacccg ctggctgcga ttgttatgat aagtttccca gccatctgta cggtcaaatt
      541 tgttcttgtc gaggttatac actttgtctg ttgcaaggaa gggagaatgg ttagtattga
      601 acaaataacc cgaaggcgga ttgatatatt gtggtagttc ctttattgat ttgaattcag
      661 tccataaagt agcagaagtg ttgcctggca gcgtactctt ccaattatat ttcgggtcag
      721 gatttctttt gggcattttt ccattgctta tataaaaaat ggtatcgtac cggtcagcat
      781 acatgatgtt gaacatgggc aggctggtca tgctgagggc cttgtagaac tctgtaaagt
      841 tttttgcctt gttcatcctg taccactgtt caagggcttt gatatccata gtggcaggta
      901 cccgcaatga gaaaacccct tttcctgtct tcaacgtagc gccgtattta ctccagtaaa
      961 tctttttgct gatggtaacc ggtataccct tcactttcag ctttgcttta ttcacttcaa
     1021 gatctatcca ttgcccgtcg aatttgtatt gatttttatt tgccgggttc atttccaact
     1081 ggtatatatc caatttatcc tggtaattga ccgtatgtgc ccaacccaga ttttcattcg
     1141 ttccgtgaaa aatgaggcac ccccccggaa ataaaccgcc gagcatattc cacccttgtt
     1201 cactttgcaa atgtgcctcg taaaaagcca ccgggcctgt attcggttga tgcgcattga
     1261 tggccaggaa actttcgccg gtggttgttt tcaccgagtt catcgcaaag gcattggaac
     1321 cttgcgggtt aaagccggga atagtggcta cttttccgcc cagtatctgc ggtaatgcat
     1381 catcaactcc gcaaaaaaga ctgatggaaa aaacgacagc tgtcatatag tctttctctg
     1441 taaagggaaa tgccttttta tattttactt tcccggggta cttttttgca taagcattca
     1501 aacccgccac atac
//
LOCUS       DAORLS010000475         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_41729_length_1514_cov_1.374914, whole genome shotgun sequence.
ACCESSION   DAORLS010000475 DAORLS010000000
VERSION     DAORLS010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41729_length_1514_cov_1.374914"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="PK977_20135"
     CDS             complement(<1..>1105)
                     /locus_tag="PK977_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20505.1"
                     /translation="SDYMRQLLCDDKNRIWVMAQGGLVTMIDENRQFHKISLNDSSGR
                     ITARWMLKTKEQGIVLFTRKGFFFLSPAINSSHKDSLTRNDFSFVTIRGFDSLQALPF
                     SQIEPFDENNYILSRDEGLYKINFREKRVEKKYELPGLYILLQWQPGELLAFDKKNPE
                     LQSIHLDSRQTSWPLRGVKDQYGHAFTAKIVDAKMLNEELLALTTAMDGMYLFNTRTR
                     SLIRCRHSAADPTTIVNDWPTVITPGINGWVFIGAPPNGISYFKYNAAIGQQTVFQDN
                     KGNSYDGHISKITTLDNDVFYIATGNNLLKWRRSTNSSSFVDYTKMNGEQMMYKDHTS
                     SLEFDAYGRLWFNTGQNGVFVLNKNDQTVHQFQY"
     assembly_gap    1106..1229
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          355 a          327 c          304 g          404 t
ORIGIN      
        1 atattgaaac tggtgaacgg tctggtcgtt tttgttcagg acaaaaacac cgttttgacc
       61 tgtgttgaac cacagcctgc cataggcatc aaactcgagt gaactcgtat gatccttata
      121 catcatttgc tcaccgttca ttttggtata atcaacaaag ctgctgctgt tggtgcttct
      181 tctccacttc aatagattgt ttccggtggc tatataaaaa acatcattat ccagtgtggt
      241 gatcttgctg atatgcccgt cgtaactatt gcctttatta tcctggaaaa ccgtttgctg
      301 gccaatggcc gcattgtatt tgaaataact gataccgttg ggaggagcgc caataaaaac
      361 ccacccgttt attcccgggg tgatcacggt aggccagtca ttcacaattg ttgtcgggtc
      421 tgcagcgctg tgccggcaac ggataagtga tctcgtccgg gtattaaaaa gatacatacc
      481 gtccatagct gtcgttagtg ccagcaactc ttcattcagc attttggcat caacgatctt
      541 tgcggtgaaa gcatggccat actgatcctt tactccccgc aggggccagc tggtttgccg
      601 gctgtccagg tgaatagatt gcagctcggg attcttcttg tcaaaggcaa gcaattcacc
      661 gggctgccac tgcaggagaa tatacaggcc gggcagctca tatttttttt ccaccctctt
      721 ttccctgaaa ttgatcttat ataaaccctc gtccctgctt agtatataat tgttttcatc
      781 aaacggctcg atctgtgaaa agggtaatgc ctggagtgaa tcaaagcctc ggatagttac
      841 aaacgaaaaa tcattccttg tcagcgaatc tttgtggctg ctgtttatag caggtgaaag
      901 aaagaaaaaa cctttgcggg taaaaagtac aataccctgc tctttggttt tcagcatcca
      961 tcgggcagtg attcttccgc tgctgtcgtt caaacttatt ttatgaaact gcctgttctc
     1021 atcgatcatt gtcaccaggc caccctgcgc catcacccat atcctgtttt tatcatcaca
     1081 caacaactgc cgcatataat cgcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttgtcagata atccgtgtgc cgtattcagc
     1261 tggtaaaaat ggatattggc ccggtgggga cgggattgag atagcagggc tgcgggcaaa
     1321 agcaccaggc tgatccagag aaaagcagat cccggtccta tgttttttag ccatcgcata
     1381 ccttcttaaa aataacttat ttgccctaaa agacagcttg ccgctggcct ggtactactg
     1441 aacggtagct gccgctgatt gaacggcata tcattattaa tattaactgc atggaagaat
     1501 taggttgcac actt
//
LOCUS       DAORLS010000476         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_41796_length_1512_cov_1.796843, whole genome shotgun sequence.
ACCESSION   DAORLS010000476 DAORLS010000000
VERSION     DAORLS010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41796_length_1512_cov_1.796843"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>593
                     /locus_tag="PK977_20140"
     CDS             <1..>593
                     /locus_tag="PK977_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF20506.1"
                     /translation="AFTGLSKETWLLSLIMLINRSGTMVIPFMTLYLTSPEMGYSVGE
                     AGIVFGLFGAGAFSGAWLGGRLTDKIGFYPVQLIALSGGGILFMILGQMNTYPLICLF
                     TYLLSFVNEAFRPANSTAIAFYSKEENRTRSYALNRLAINLGWAVGSGLGGFLANINY
                     ELLFWVDGCTNIGAAVLMWLVLKPVAYKPSARKGSNS"
     assembly_gap    594..643
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <644..>1022
                     /locus_tag="PK977_20145"
     CDS             <644..>1022
                     /locus_tag="PK977_20145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF20507.1"
                     /translation="VITLLFASCFFQLFNNLPLFFEKEMHFSKPFIGILMSANGVIIA
                     LLEMVLIYKLEGRRQNIVYITTGVFIVGLSFLLLNAPGPAAVVASGMIFLVTIGEILS
                     MPFMNSYWIARTQPSNRGQYAALY"
     assembly_gap    1023..1043
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1229..>1512
                     /locus_tag="PK977_20150"
     CDS             1229..>1512
                     /locus_tag="PK977_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20508.1"
                     /translation="MKNTLILIIATTTLTLLSCKQKKEILTAKDVQAVISRFDNGWKN
                     KDTAAVDSALAVNYIYFTQSGGTYERQNVMKTAASPEYKLEKYSREEISYR"
BASE COUNT          383 a          311 c          331 g          416 t
ORIGIN      
        1 atgcatttac aggcctgtca aaagagacct ggcttttatc gttgatcatg ctgatcaaca
       61 gaagcggcac catggtcatc cccttcatga ccctttatct caccagcccg gaaatgggat
      121 actctgttgg tgaagctggg attgttttcg ggctatttgg cgcaggagca tttagcggtg
      181 cctggctggg tggaaggctg accgataaaa ttggatttta ccccgttcaa ttaatagcat
      241 tatccggcgg tggtatactt tttatgattt tggggcaaat gaatacctac cccctgattt
      301 gcctcttcac ctacctgcta agcttcgtta atgaagcctt ccggccggcc aattcaactg
      361 caattgcttt ttactcaaaa gaggagaaca gaacccggtc atacgcattg aacaggctgg
      421 ccataaattt gggatgggct gtgggaagcg gcctgggtgg ctttttggcc aatatcaatt
      481 atgaactgct tttctgggtg gatggatgta ccaatattgg agcagctgta ttgatgtggc
      541 tggtactaaa acctgttgct tataagccgt ccgccagaaa aggtagcaat agcnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtcatt accctgctgt
      661 ttgcaagctg tttctttcag ctctttaata atttgccttt attctttgaa aaggaaatgc
      721 acttttcaaa accatttatc ggcatactaa tgagtgcaaa cggagtgatt atagccctgc
      781 tggaaatggt attgatttat aaattagaag gcaggcgcca gaatattgtg tacataacca
      841 ctggtgtttt catcgttgga ctttcgttct tactgttgaa tgcgccaggc ccagccgctg
      901 tagttgcatc gggaatgatt tttctagtca ctattggaga gatattatcg atgcccttta
      961 tgaacagtta ttggattgca cggacacaac cttctaacag ggggcagtat gcggctttgt
     1021 acnnnnnnnn nnnnnnnnnn nnngcacaaa cccttggccc gatgggtggt gctcaaatag
     1081 cccagcattg gggcttcagc aacttgtggt ggattacagg aggcttatgt attattgcgg
     1141 cagctgcttt taaacatttt gtccgtaact gattttaacc ttcacaatcg ttttgaaaaa
     1201 gctaataacc ggactacctt tgcatcccat gaaaaataca cttatcctga ttattgcaac
     1261 caccactctc accctgcttt cttgtaagca gaaaaaagaa atattgacgg caaaagacgt
     1321 ccaggccgtt atcagtcgtt ttgacaatgg ctggaaaaac aaggatactg cagctgtaga
     1381 ttccgcacta gcagtcaatt acatttattt tacccagtcc ggtggcacat acgaaaggca
     1441 aaatgtaatg aaaacagccg cttccccgga atacaaattg gaaaaataca gccgggagga
     1501 aatctcttac cg
//
LOCUS       DAORLS010000477         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_42410_length_1493_cov_1.869263, whole genome shotgun sequence.
ACCESSION   DAORLS010000477 DAORLS010000000
VERSION     DAORLS010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42410_length_1493_cov_1.869263"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    226..258
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            264..>1493
                     /locus_tag="PK977_20155"
     CDS             264..>1493
                     /locus_tag="PK977_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF20509.1"
                     /translation="MLKSPIEYLKGVGPQRADLLKKELGIFTFGDLLNHFPFRHVDRT
                     KLNSIGDITFATEFAQVAGVLLSVELIGQRSGKRLVAQLKDKTGIIELTWFQGLNWVQ
                     KTLQEGHSYIAFGRISFFNGQPQIIHPELELLVPGGPGAKTFLEPIYSTTEKLKSKGL
                     GGRQIGKLTQLLMGMLHEKDVPENLPETLLTQLKLISRFKAYRQLHFPQSSADYEDAV
                     KRIKFEELFIAQLRMNLLRSSRHRFSKGVVFDKVGDLFNDFFKNHLPFGLTGAQKRVI
                     KEIRTDTARGRQMNRLLQGDVGSGKTIVAVLIMLLAVDNNYQASLMAPTEILAQQHFQ
                     TISELLKGVHVQVALLTGSTKSAARKKILNELQEGTIKILIGTHALIEDAVQFNNLGI
                     VIIDEQHRFGVAQRAKLW"
BASE COUNT          439 a          276 c          336 g          409 t
ORIGIN      
        1 attcggctgg ctgtttgttc gatcgggtaa acgagcaggg ctttttacta ttcctttata
       61 ctttgtattc atgaattatt gtcttgtaaa aggattttta agatatatca ggggaaaaca
      121 atcagtgctt tgggaaaaat cagtcaggca gatggcctag tctttctatt atgactttat
      181 tgccacgata atataggtac acaacaatta attttgtagt aaacannnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnncc caaatattaa agagcccgat tgaatacctg aaaggggtgg
      301 gcccgcaaag agctgatctt ttaaaaaagg aattgggcat cttcaccttt ggtgatttgt
      361 tgaatcattt tccattccgt catgttgaca gaacaaagct gaattctatt ggtgacataa
      421 cctttgcaac tgagtttgcc caggtggccg gggtgttgct aagtgtcgaa ctgatcggtc
      481 aacgttccgg taaaaggtta gtggctcagt taaaggataa aacaggcatt atcgaactca
      541 cctggtttca gggtttgaac tgggtacaaa aaactttgca ggagggccat tcatacattg
      601 cttttggaag aataagcttc tttaatgggc aaccccagat tattcatccg gaattagagc
      661 tattggttcc gggtggtccg ggcgccaaaa ctttcctcga gcccatttat tctaccactg
      721 aaaaattaaa gtctaaagga cttggcggaa ggcagatcgg caaacttaca caactgctga
      781 tgggtatgtt gcatgaaaaa gatgtgcctg aaaatttgcc ggagaccttg ttgacacaat
      841 taaagttgat tagtcgcttc aaagcatacc gtcagttaca tttcccgcaa tcttccgccg
      901 attatgagga tgcggtgaag cgtataaaat ttgaggaact tttcatcgca cagcttcgaa
      961 tgaacctgct gcggtcaagc cgccaccgat tttcaaaagg ggtggttttt gataaagtag
     1021 gtgatctttt caatgatttt tttaaaaatc atcttccttt tggactgaca ggtgcacaaa
     1081 aaagagtcat caaggaaata cgtacagata cggcaagggg cagacaaatg aaccggctct
     1141 tacagggaga tgtcggtagt ggtaaaacaa ttgtagcagt attgataatg ttgctggcag
     1201 tggataataa ctaccaggct agcctgatgg ctccgacaga aatactggcg cagcaacatt
     1261 ttcaaacgat cagcgaatta ttaaagggag tgcatgttca ggtagcatta cttacaggct
     1321 ctaccaaatc agctgccagg aaaaaaatat taaatgaact gcaagaaggc actataaaaa
     1381 ttttgatcgg cacccatgca ctgattgaag atgccgttca gttcaataac ctcggtattg
     1441 tcatcattga tgaacagcat cgctttggcg tggcacaaag agcaaagctc tgg
//
LOCUS       DAORLS010000478         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_46012_length_1390_cov_1.852434, whole genome shotgun sequence.
ACCESSION   DAORLS010000478 DAORLS010000000
VERSION     DAORLS010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46012_length_1390_cov_1.852434"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>367
                     /locus_tag="PK977_20160"
     CDS             <1..>367
                     /locus_tag="PK977_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20510.1"
                     /translation="QGRSQTFTTNISCLDANWNDQASSIVVESSIFQPGGNNNDYVTF
                     YSDCYSRGFSRTLRPGTYTGNDLANLKNNISSFSIYGNLRVRVYINNESATGYYNTFD
                     ASQTCLSNSYNDKISSLVIE"
     assembly_gap    368..383
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <384..966
                     /locus_tag="PK977_20165"
     CDS             <384..966
                     /locus_tag="PK977_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20511.1"
                     /translation="GTGGGSGNTGRYVTFYEDCNYGGNNIRLQPGYYQGDKLGMFRYD
                     ISSVEVPSGMRVKVFMNESLSGSSYTISENNYCLNSTLNNRIGSFVIEETGYGGNNNY
                     PPNTGSAVVIYTDDNYRGQSATLYPGTYSTMSQAGFPDNALSSLSVPAGFRVVLYEFE
                     NFRGKSYTITQSKTGFSFSGWNDKTSSIAVYRD"
     assembly_gap    1038..1047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1048..>1390)
                     /locus_tag="PK977_20170"
     CDS             complement(<1048..>1390)
                     /locus_tag="PK977_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRF20512.1"
                     /translation="KIAGLQAFRQAFQQADPQILEPIYHVEVLCPDDLTGAVMGDLQS
                     RRAIVEGIDSEGHFQKVIARVPLAEMEGYSSSLRSITQGRAKFKMRFEAYEPVPFDLQ
                     RRLIDEYSKTAK"
BASE COUNT          367 a          321 c          294 g          382 t
ORIGIN      
        1 cagggccgtt cacaaacatt cactactaat atttcctgcc tggatgccaa ctggaatgac
       61 caggcttctt caatagttgt agaaagttcc atttttcaac ccgggggcaa taacaacgac
      121 tacgttactt tttacagcga ctgttattcc aggggatttt cccgcacact gcggccgggt
      181 acttacactg gcaatgatct tgcaaatcta aaaaataaca tttcttcctt ttccatttat
      241 ggaaatcttc gggtaagagt ttatataaac aatgaaagtg caaccggtta ttacaatacg
      301 tttgatgcca gccaaacctg cttgagcaat tcctataatg acaagatcag ttctttggta
      361 attgaatnnn nnnnnnnnnn nnnaggtacc ggtggtggca gcggcaatac cggcaggtat
      421 gtaacttttt acgaagattg taactatggc ggtaataaca ttcggctgca accaggatat
      481 taccagggcg ataaactggg gatgtttcgc tatgacatct cctccgttga agttccgtct
      541 ggtatgcggg taaaagtttt tatgaacgaa tctttatcgg gttcgtccta taccatctct
      601 gaaaataatt actgcctgaa cagcacatta aataaccgca tcggctcttt tgttatcgaa
      661 gaaacaggat atggcggaaa taataattac ccgcccaata ccggcagcgc tgttgtaatt
      721 tatactgatg ataattaccg gggccagtcg gccactcttt atcccggtac atacagcacc
      781 atgtcacagg caggttttcc ggacaatgcc ttaagttcac tatcagtccc tgccggcttc
      841 agggtagtat tgtatgagtt tgaaaatttc cggggtaaaa gctataccat tacccaatca
      901 aaaacaggct tctctttttc aggatggaat gacaaaacat catccatcgc tgtttacagg
      961 gattgataaa aaaccgcccc ggtattcaat cggggcggtt gatagtagca gtccgtaagc
     1021 aaacttatct caggcaannn nnnnnnnttt cgctgttttg ctgtattcat cgatcagcct
     1081 gcgctgcaga tcaaacggca ctggttcata ggcttcaaac cgcattttaa atttggccct
     1141 gccctgtgtg atggaacgca gggatgaaga ataaccttcc atttcagcca gcggcacccg
     1201 ggctatcact ttctggaagt gtccttcgct gtcgattccc tctacaatgg cacgacgact
     1261 ttgcaggtca cccattactg caccggtaag atcatcgggg cacaatactt ccacatgata
     1321 tatcggttcc agtatctgcg gatcagcctg ctggaatgcc tggcggaatg cctgcaatcc
     1381 ggctatctta
//
LOCUS       DAORLS010000479         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_46233_length_1384_cov_2.112114, whole genome shotgun sequence.
ACCESSION   DAORLS010000479 DAORLS010000000
VERSION     DAORLS010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46233_length_1384_cov_2.112114"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK977_20175"
     CDS             complement(<1..527)
                     /locus_tag="PK977_20175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF20513.1"
                     /translation="MIRRIIFFVCFSFTAMLVFAQAEVKVMSYNIRLDVKSDGENWWE
                     NRKDKVATLINYYETDFIGLQEVLHHQLNYLKEQLNGYDNIGVGRDDGKQAGEYSCIF
                     YKTDKYTLIKQSTFWLSPTPDTPSKGWDAALNRVCTYGLFKDKKTKKLLWVFNTHFDH
                     IGKIARLESAKLINQ"
     gene            complement(531..>993)
                     /locus_tag="PK977_20180"
     CDS             complement(531..>993)
                     /locus_tag="PK977_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRF20514.1"
                     /translation="YWEECKNQALINYQWCVDNPQKYKGYGPDNWGLTSSYSVNGYAG
                     HAPTMQRDLGVISPTAALSSFPYTPKQSMAALKHWYNDMKERLWGPYGFYDAFSEEHN
                     WFLPHYLAIDQGPIVVMMENYRSGFIWNLFMSCPEVKTGLKKLGFTSPYIK"
     assembly_gap    994..1004
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1005..>1384)
                     /locus_tag="PK977_20185"
     CDS             complement(<1005..>1384)
                     /locus_tag="PK977_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRF20515.1"
                     /translation="AARADKLWREVEFNWYRNGQNILYWHWSPNYGWQMNFPVRGYNE
                     CLIMYVLAASSPTHGVPAEVYHEGWADNGRIKDVNKPNGIAANYPYKLQMKHQGDAWN
                     GGPLFWAHYSFLGLDPRGLKDKYA"
BASE COUNT          369 a          321 c          242 g          441 t
ORIGIN      
        1 ttttgattga tcagtttagc tgattccagc cgggctattt ttcctatatg atcaaaatgg
       61 gtgttgaata cccacagcaa tttctttgtt tttttatcct tgaataaacc gtaggtgcat
      121 acccggttga gtgcggcatc ccagcccttg gaaggcgtat ccggtgttgg cgaaagccaa
      181 aacgttgatt gcttaattaa tgtgtacttg tctgtcttat aaaaaataca ggaatattct
      241 cctgcttgtt ttccgtcatc tcgtcctaca ccaatattat catacccgtt tagttgttct
      301 ttcaggtaat tcaactggtg atgcaatact tcctgtaagc cgataaaatc ggtttcataa
      361 taatttatca gtgttgctac tttatccttt ctgttctccc accagttttc gccgtctgat
      421 tttacatcca gccggatatt ataactcatc actttaactt ctgcctgtgc aaagacaagc
      481 atggcagtaa acgaaaagca aacgaaaaaa ataattcttc tgatcatcgc ttattttata
      541 taaggactgg tgaatcctaa tttttttaat cctgttttta cttccgggca actcatgaac
      601 aaattccaaa taaaccctga acgatagttt tccatcatca ctactattgg tccctggtca
      661 atagccagat aatgcgggag aaaccagtta tgctcttcac tgaaagcatc ataaaaacca
      721 tacgggcccc acaatctttc tttcatatca ttataccaat gcttcagtgc tgccatagac
      781 tgctttggtg tatagggaaa tgaggataag gctgccgttg gcgatataac tcccaggtct
      841 ctttgcatag ttggtgcatg accggcatag ccatttacag agtagctgga ggttaatccc
      901 cagttatcgg gaccatagcc tttgtacttt tgaggattat caacacacca ttgataattg
      961 atcaaagcct ggtttttaca ttcctcccaa taannnnnnn nnnnctgcat atttatcctt
     1021 caaacctctt ggatctaatc ctagaaaaga gtagtgagcc caaaacaacg ggccgccatt
     1081 ccatgcgtca ccctggtgct tcatttgtaa tttataagga taattagcag caataccatt
     1141 tggtttgttt acatccttta tcctgccatt atctgcccag ccctcatgat acacttcagc
     1201 cggcacccca tgtgtgggtg aagatgcagc cagtacatac attatcaggc attcattata
     1261 accccttacc ggaaaattca tctgccatcc atagttgggg ctccagtgcc agtataaaat
     1321 attttgcccg tttctgtacc agttaaattc aacctctctc cataacttat cggctcttgc
     1381 cgct
//
LOCUS       DAORLS010000480         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_46236_length_1384_cov_1.938299, whole genome shotgun sequence.
ACCESSION   DAORLS010000480 DAORLS010000000
VERSION     DAORLS010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46236_length_1384_cov_1.938299"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>372)
                     /locus_tag="PK977_20190"
     CDS             complement(<1..>372)
                     /locus_tag="PK977_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20516.1"
                     /translation="TGGSFYSDVRDEAIALNALIDVDPGNAQIGTMVKHVADKLKRRQ
                     WLSTQERAFAFLALGKHARTANNSTATAEIKVNGKVIAKVDGGQWTGDMKALGSNNAE
                     ITVKGNGRLYYFWQSEGISSSG"
     assembly_gap    373..397
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<398..>668)
                     /locus_tag="PK977_20195"
     CDS             complement(<398..>668)
                     /locus_tag="PK977_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20517.1"
                     /translation="INNRLKNKETITYYYNRDQNKKIAPKEVAYGLYVLALANRSNVP
                     AMNYYKANPALLALDSRYLLAAAYATAGDKRSFTAMLPGSFAGEES"
     assembly_gap    669..687
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<688..>997)
                     /locus_tag="PK977_20200"
     CDS             complement(<688..>997)
                     /locus_tag="PK977_20200"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20518.1"
                     /translation="LVQYPYGCTEQTISAAFPQIYYGDLADLMMLNKQQNKLNANSNI
                     MEAIRKIKMRQLYNGAVTLWDDEGKEDWWTTIYAAHFLLEAKKAGFDVDNSLMETILN
                     Y"
     assembly_gap    998..1012
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1013..>1384)
                     /locus_tag="PK977_20205"
     CDS             complement(<1013..>1384)
                     /locus_tag="PK977_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20519.1"
                     /translation="DKPATVSAVISVEGPMKVLGGNSQSVNLSAKSEGRASFRIVADP
                     TINVGKVTVTVNGMGEKFAEATEIAVRPPSTLQKITGSGSITGGNMQKINIGVSDFMP
                     GSINYSLVVSRSPALELGEQLK"
BASE COUNT          334 a          320 c          251 g          420 t
ORIGIN      
        1 ccgctgctgc tgataccttc gctctgccag aaataataca accggccgtt tccttttacc
       61 gttatttctg cattgttact tcctaaagct ttcatgtcac ctgtccattg accaccgtca
      121 actttggcaa ttacttttcc attgaccttt atttcagctg tggcagtgga attattggct
      181 gttcttgcat gtttgcctaa tgccaggaaa gcaaatgccc tttcctgggt actcagccac
      241 tggcgtctct tcaatttatc cgccacatgt tttaccatag tacctatttg tgcatttccg
      301 gggtcaacat caataagtgc attcaatgca atagcctcgt ctcttacatc ggaataaaaa
      361 ctgcctccgg ttnnnnnnnn nnnnnnnnnn nnnnnnngat tcttctccgg caaatgatcc
      421 aggaagcata gctgtgaatg atcgcttatc accagctgtg gcatacgccg ctgcaagcag
      481 ataacggctg tccagggcca gcaaagcagg attagcttta taataattca tggccggtac
      541 attgcttcga ttagcaaggg ccaaaacata caacccgtag gccacttctt ttggggcaat
      601 tttcttattt tgatcacggt tgtaataata tgtaatggtt tctttattct tcaaccggtt
      661 gttgatgtnn nnnnnnnnnn nnnnnnngta gttaagtatt gtttccatca ggctgttatc
      721 aacatcaaag cctgctttct ttgcttctaa aagaaaatgc gctgcataaa tggtggtcca
      781 ccagtcttct ttcccctcat catcccacaa ggtaacggcg ccgttgtaca actgccgcat
      841 tttgatctta cgtatggctt ccattatgtt gctgttagcg tttaatttat tctgttgctt
      901 attgagcatc attaaatctg caaggtcgcc gtaatagatc tgcggaaaag ctgctgatat
      961 agtttgctcg gtacaaccat agggatattg taccaggnnn nnnnnnnnnn nnttttaatt
     1021 gttcacccaa ttcaagtgca ggagaacggc tgacaacaag gctatagtta atactgcccg
     1081 gcataaaatc acttacacca atatttattt tctgcatatt gcccccggta atagaaccac
     1141 tgcccgttat cttttgcaaa gtagatggag gtcgtacagc aatttctgtt gcctctgcaa
     1201 atttttcacc catgccattt accgttacag ttaccttacc cacattaata gttggatctg
     1261 ccacaatacg gaaagaagct cttccttctg acttagcact aagatttaca gactgactgt
     1321 ttccacccag cactttcatc ggaccttcca ctgaaatgac agcagaaaca gttgcaggtt
     1381 tatc
//
LOCUS       DAORLS010000481         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_47119_length_1360_cov_2.194636, whole genome shotgun sequence.
ACCESSION   DAORLS010000481 DAORLS010000000
VERSION     DAORLS010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47119_length_1360_cov_2.194636"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK977_20210"
     CDS             complement(<1..538)
                     /locus_tag="PK977_20210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF20520.1"
                     /translation="MEPFFWISLIVLFYSYIGYGLIIFLLNNIRVLFSGNINFLQTEH
                     LPVTLIITAYNEANLLPLKISNTLAIDYPANKLTVIFVTDGSSDNSAELISKFPSLQL
                     MHLPERRGKYAAVKRAMQQVKTPIVIFSDANTMLNPECINRIVTHYADPKVGGVAGEK
                     KILRNNYASAVGEAEGLYW"
     gene            723..>1360
                     /locus_tag="PK977_20215"
     CDS             723..>1360
                     /locus_tag="PK977_20215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRF20521.1"
                     /translation="MKSHYYLLQIDLSLITYKFALLCFLSAFVVTLITIPPVINLIRK
                     YRLYDVPNARKEHSTPIPTMGGVAIVAGMMAALFLWFPFSNQPGQISFFFSVIVLLAL
                     GIMDDLKDLSARYKFVVQFALASLVAVAGIRITSFDGLFGIYELPLTAQYTFTILAIV
                     GITNAFNLIDGIDGLAGGLGFMSLVILGLFLTMSGDVNTALIAFSLAGGMLA"
BASE COUNT          365 a          297 c          256 g          442 t
ORIGIN      
        1 gccagtataa gccctctgct tcacccacgg cagatgcata attgtttctc aaaatctttt
       61 tttcgccggc aactcctcca accttcgggt ctgcataatg tgtaacaata cggtttatgc
      121 actccgggtt aagcattgta ttagcgtcgc tgaatatgac aatcggggtt ttcacctgct
      181 gcatggccct tttcacagct gcatactttc cccttctttc cggcaggtgc attaattgta
      241 aagaaggaaa cttgcttatc aattccgccg aattgtccga tgagccatct gtaacaaata
      301 tcacagtaag cttatttgcc gggtaatcaa tagccagcgt attactgatc tttagcggta
      361 acaggtttgc ctcattatac gctgttataa tcaatgttac cggcaaatgt tctgtttgta
      421 aaaaattaat atttcctgaa aacaataccc ggatattgtt taataaaaag atgatcagcc
      481 cataaccgat atagctatag aataaaacaa tgaggcttat ccaaaaaaag ggctccatta
      541 ccatcacctg aagagaccgg aaaattacgg tccgtcagca gcaaatatcc gctgctttac
      601 cggaatactg cagattctta cgtttaaact attattttca ggaagatata gattaaccca
      661 cttgcttttt tacataatct gctctacttt gcatacacca atatccattc gaaacctgta
      721 ttatgaaaag ccactattac ctgctacaga tcgatttgtc attgatcacc tacaaatttg
      781 ccttactctg cttcttatcc gcatttgttg ttactttaat aaccatacca cccgttatta
      841 atctgataag gaagtaccgg ctatatgatg tgcctaatgc ccgtaaagaa cattcaaccc
      901 cgatacccac tatgggtggc gtagctattg ttgccggtat gatggcagct ttatttttat
      961 ggtttccgtt tagtaatcag cccggtcaga tcagtttctt tttctcagtt attgtattgc
     1021 tggcccttgg tattatggac gatttaaaag acctttcagc caggtataaa tttgttgtac
     1081 aattcgcact tgccagtttg gtggcagttg cgggtatccg aatcacttca tttgatggcc
     1141 tgtttggtat ttatgaactg ccattaacag cacaatatac ctttaccatt ttagccatag
     1201 tcggaataac taatgctttc aatttaattg atggtattga cggccttgcc ggtggattag
     1261 gctttatgag cctggtaatc ctgggacttt tcttaactat gagcggagat gtgaatacag
     1321 ctctgatcgc attctcattg gctggtggaa tgctggcctt
//
LOCUS       DAORLS010000482         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_47603_length_1348_cov_1.408353, whole genome shotgun sequence.
ACCESSION   DAORLS010000482 DAORLS010000000
VERSION     DAORLS010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47603_length_1348_cov_1.408353"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>746
                     /locus_tag="PK977_20220"
     CDS             <1..>746
                     /locus_tag="PK977_20220"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF20522.1"
                     /translation="TKRLRKIYSGPTSQWPRPLVDEGVKWTELGLLPESPVAPYKDSL
                     QHIIALGKSLFFDTRLSGSGKISCATCHQPELNWTDGKEKSIGHEGAVNKRNSPTIQN
                     SWFYIRLFWDGRSRDLQDQAFAPINSESEMHSEMPDVMRKLRKSKGYKELFKKAFGDE
                     QIEPDRMTEAIAVFEKTIVSRKSRFDEFMEGNKKALSNSELRGLHIFRTKAKCMNCHS
                     GPLFTDNSFHNIGFSGNDEGHYKVSHKEED"
     assembly_gap    747..937
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1054..>1348
                     /locus_tag="PK977_20225"
     CDS             1054..>1348
                     /locus_tag="PK977_20225"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRF20523.1"
                     /translation="MKKLFLTLIIAGFGFIANAQPPEGDAKVGEWYGEKLNTEGALNI
                     NDVVPKLEGGSEFDNVKIKAKITEVCPKKGCWLKLELNDGKTAMVKMKDYGFFL"
BASE COUNT          379 a          256 c          261 g          261 t
ORIGIN      
        1 acacaaagcg gttaaggaaa atttattccg ggccaacctc acaatggccc aggccattgg
       61 ttgacgaagg agtaaaatgg acagaactgg gcctgcttcc ggaaagcccc gttgccccgt
      121 acaaagactc gctgcaacat attattgcac tcggcaaatc ccttttcttc gatacccggc
      181 taagtggttc cggaaagatc agctgcgcca cctgtcatca gccggagcta aactggaccg
      241 acggaaaaga aaaatcaatc gggcatgaag gcgcagtcaa taaaagaaat tctcccacta
      301 tacaaaacag ctggttttac attcgcctgt tctgggatgg ccgttctaga gacctgcagg
      361 accaggcttt tgcccctatc aacagcgaat cagaaatgca cagtgagatg ccagacgtaa
      421 tgcgaaagct aaggaagagc aaaggctata aagaactttt caaaaaagcc tttggcgatg
      481 aacagattga accagaccga atgacagaag ccattgccgt atttgaaaaa acgatagtaa
      541 gccgaaagag ccggtttgat gaattcatgg aaggcaacaa aaaagcttta agcaatagtg
      601 agctcagggg acttcatatt ttccgcacaa aggctaagtg catgaattgt cacagtggtc
      661 cgcttttcac cgacaactcc ttccataata ttggcttttc cggtaatgat gagggacatt
      721 acaaagtgtc gcataaagaa gaagatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat gccatttctg cccctcccgc
      961 agattttcaa aaacctgttt tacccgagta ggcaactatt tgcggtgaaa agctatcttt
     1021 gatttccttt tcaaacaacc aaaaaaaaat aacatgaaga aactgttcct aacattgatc
     1081 attgccgggt tcggctttat tgccaatgca caaccacctg aaggagatgc aaaggtgggt
     1141 gaatggtatg gcgaaaaatt aaacacggaa ggcgccctta acatcaacga tgtagtaccc
     1201 aaactggaag gcggctctga gtttgacaac gtaaagatca aagcaaaaat taccgaagta
     1261 tgccctaaaa aaggctgctg gctgaagctc gaattgaatg atggcaaaac agcgatggta
     1321 aaaatgaaag attatggttt cttcctgc
//
LOCUS       DAORLS010000483         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_48219_length_1332_cov_2.287392, whole genome shotgun sequence.
ACCESSION   DAORLS010000483 DAORLS010000000
VERSION     DAORLS010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48219_length_1332_cov_2.287392"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PK977_20230"
     CDS             complement(<1..1193)
                     /locus_tag="PK977_20230"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRF20524.1"
                     /translation="MANKKQATAKSGLQFSRRFTRDDVNVYDMFEYDYRTSVIRNPNG
                     ETVFEMNNVEVPKQWSQIATDILAQKYFRKAGVPQPDGSLGRETSAKQVAHRMANCWR
                     VWGERYGYFASEKDAQVFYEELVYSILNQMCVPNSPQWFNSGLYESYGITGKPQGHYY
                     VDPNDGQLKKSVNAYERPQPHACFILSVDDDLVNEGGIMDLWVREARIFKYGSGVGTN
                     YSNLRGEGEKLSGGGSSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPEIVD
                     FINWKVEEEKKVAALIAAGYPSGYEDEAYRTVSGQNSNNSIRIPNSFFKVLEEDGDWE
                     LKGRMDGRVMKKVPARELWNQIAYAAWRCADPGTQYDTTINEWHTCPAGGPIRASNPC
                     SEYMFL"
BASE COUNT          348 a          350 c          276 g          358 t
ORIGIN      
        1 aggaacatat attcagagca gggattggaa gccctgatag gaccacctgc cggacaagta
       61 tgccactcat tgatagtagt atcatactgt gtacccgggt cagcgcaacg ccatgccgca
      121 taagctatct ggttccagag ttctcttgcc ggaacttttt tcattacacg gccatccatc
      181 ctgcccttca attcccagtc accatcctct tccaatactt taaagaatga gttgggtata
      241 cggatagagt tattggagtt ctgtccgctt actgttctgt atgcctcatc ttcataaccg
      301 gaaggataac cggcagcgat caatgcagca accttcttct cctcttccac tttccagtta
      361 atgaaatcaa cgatctccgg atgatccagg tctaaacaaa ccattttggc ggcacgacgg
      421 gtggtacccc cggacttgat agctccggct gcacggtcac caatttttaa aaaactcatc
      481 aggccggaag aactgccgcc accacttagc ttctcacctt ctccacggag attagaatag
      541 ttggttccca caccagagcc atatttgaaa atccttgctt cccgaaccca cagatccatt
      601 ataccgcctt cattcaccag gtcatcatcc acactaagga tgaagcaggc atgcggctgg
      661 gggcgttcgt atgcattgac agatttcttt agctggccgt cattcggatc aacgtaataa
      721 tggccctgtg gcttacccgt aattccgtag ctctcataca agccggaatt aaaccactgc
      781 ggactattag gcacacacat ctggttaagg atgctgtaca ccagttcctc gtagaaaacc
      841 tgtgcatctt tttcggaagc aaaataacca tagcgttctc cccacaccct ccagcaattg
      901 gccatgcgat gtgctacctg tttggcagaa gtttccctgc ccaggctgcc atcaggctgg
      961 ggtacaccgg ctttacgaaa atatttttgg gcaagaatgt ctgttgcaat ctggctccac
     1021 tgtttgggaa cttctacatt gttcatctcg aacaccgttt caccatttgg gttgcggatc
     1081 accgaggtac ggtagtcata ctcaaacata tcatacacat tcacgtcatc ccgggtaaag
     1141 cgacgtgaaa actgcaagcc gcttttggcg gttgcctgct ttttattggc cataactaaa
     1201 aaaatttaac gggttttggt cctggttcaa aaatctatct cccaagcggg agacacttcg
     1261 aaagtatttt ttacaaacgg taattcaaac cttcaaatat taacattgtg tggaaaagca
     1321 atgtgcaaaa tg
//
LOCUS       DAORLS010000484         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_49051_length_1311_cov_1.986465, whole genome shotgun sequence.
ACCESSION   DAORLS010000484 DAORLS010000000
VERSION     DAORLS010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49051_length_1311_cov_1.986465"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK977_20235"
     CDS             complement(<1..707)
                     /locus_tag="PK977_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20525.1"
                     /translation="MIKLRLLLLGFGLISSFLAVKGQRTYASNSVLASGNWYKLSISS
                     AGIYKVDLAFLAALGINTSNLSSSSIKLFGNGGQMLPENNAVSRTDDLQEVAIYMADG
                     GDGIFSGADFFLFYAPGPDEWLKDSVNQRFSHRKNIYSDKSYYFLTIGNNGKRIITAP
                     LVTSPAITISSFSERYFHELDTVNFLGSGKEWYGEEISGLPGRSLTRNFSFNIPGIVQ
                     NSVITVRTECIARSAGVS"
     gene            895..>1311
                     /locus_tag="PK977_20240"
     CDS             895..>1311
                     /locus_tag="PK977_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF20526.1"
                     /translation="MKNFTILLSCVVLLASCKGKGLFGKKYDKSDVTGWNYNDKNQGG
                     FQVSKAKEQGLGPGLVFVQGGTFTMGQTEEDVMGDWNNIPRRVSVPSFYIDRTEVANV
                     HYREYLHWLYKVFDPSDPANAKILEGALPDTLCWRSE"
BASE COUNT          386 a          301 c          268 g          356 t
ORIGIN      
        1 gaaacccctg cagatcttgc tatacactct gtccttacgg taataactga attttgaaca
       61 atgccgggta tattgaaaga aaaattcctt gttagcgatc gtccgggcaa accggaaatc
      121 tcctcgccat accattcttt accactaccc agaaagttaa ctgtgtcgag ttcatgaaaa
      181 tatctttcgg aaaatgagct gatagtgatg gccggtgagg tgacaagcgg agcagttatg
      241 atccgttttc cattattccc gatggttaaa aaataatacg acttgtcact ataaatgttc
      301 tttctatgag aaaatcgctg attgactgaa tccttcagcc actcatcggg tcccggtgca
      361 taaaaaagga aaaagtcagc tccgctaaag ataccatccc ccccgtctgc catataaatg
      421 gccacctcct gcaaatcatc agttcggctg accgcattat tttctggcag catttgcccc
      481 ccatttccaa atagctttat agatgaagag ctgagatttg atgtattaat acccaaggcg
      541 gccaaaaaag ccagatcaac tttataaatt ccggcagaac taatactcag cttgtaccag
      601 ttgcccgaag ccaataccga attggatgca taagtacgct gtccttttac ggctaaaaag
      661 ctgctgatca aaccgaatcc aagtaacaac aaccggagct ttatcatgct tgtaaaccaa
      721 tgggttttaa agggattttc cgcccgaaaa aaagtatttc tgcgtatctg tatattacga
      781 aagtgcctat attcgcaatc ccggcaggta aaacctttta ccaggtgcaa tatttttacc
      841 tcatccggcg ttaaaaatat tccaattcat aggtttatgc aaaaaacatt gagtgtgaaa
      901 aactttacaa tccttttatc ctgtgtggtg ctgctcgcat cttgtaaggg caaaggcctt
      961 tttggcaaga aatacgataa gtcagacgtc accggctgga actacaatga caaaaaccag
     1021 ggtggtttcc aggtatccaa agccaaagaa cagggtctcg gtcccggtct ggtgtttgtt
     1081 cagggtggta catttaccat gggacaaaca gaggaagatg taatgggcga ctggaataat
     1141 attccccgcc gtgtatctgt tccctccttc tacattgacc gcactgaggt tgccaacgtt
     1201 cactaccgtg aatacctcca ctggctgtat aaagtatttg atccttcaga ccctgcaaat
     1261 gccaaaattt tagaaggtgc actgcctgat acgctttgct ggagaagtga g
//
LOCUS       DAORLS010000485         1258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_51266_length_1258_cov_2.402328, whole genome shotgun sequence.
ACCESSION   DAORLS010000485 DAORLS010000000
VERSION     DAORLS010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51266_length_1258_cov_2.402328"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK977_20245"
     CDS             <1..227
                     /locus_tag="PK977_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HRF20527.1"
                     /translation="TESVVALAVAGLMSNEDGYIVAEAYTEIDREAKELGSTLAAPFM
                     TLSFMALLVIPHLKLSDKGLFDGDRFELIN"
     gene            356..646
                     /locus_tag="PK977_20250"
     CDS             356..646
                     /locus_tag="PK977_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF20528.1"
                     /translation="MKKLLHISAAFDQPELVQNLVERLYRRYELGYGTQMMPRGLKAL
                     ETSSTETYVSGTIAIDADNNEIINMPFITCFLFTCIKGKSEPYQLIWSSSLN"
     gene            complement(689..>1258)
                     /locus_tag="PK977_20255"
     CDS             complement(689..>1258)
                     /locus_tag="PK977_20255"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRF20529.1"
                     /translation="AMTADKLLISIFGGAFLGIGVGLIMRTGAALDGIEVLAVYTLKR
                     TSFTITEIILGINIIIFTIAGFRFGIETALYSILTYFTATRCIDYVVEGLQAYTGVTI
                     VSGKSEAIKYQLVNKLGRGITVYKGERGFLPGKYDVSAECDIIFTVITRLELRKLKNL
                     VYDVDPKAFVFANTIKEASGGIIKRRHSH"
BASE COUNT          376 a          287 c          271 g          324 t
ORIGIN      
        1 aaactgaatc agtagtagcg ctggcggtag cgggactgat gagtaacgaa gatggctata
       61 ttgtagcaga agcttatacg gagatcgacc gggaggccaa agaacttggc tccaccttag
      121 ctgcaccctt tatgacactt tcttttatgg cgctgctggt catcccgcat ttgaaattga
      181 gtgataaggg tttgtttgac ggggataggt ttgaattaat aaactaatac tttcacattc
      241 ccttaataac caaaaacggc tatcacagca tctaattcat aaacaaggtt acaaagccct
      301 ctgaagggtg gacaaagtgc agaagaaaaa gggccgaaac aaaaaccctt gagttatgaa
      361 aaagctcctg cacatctccg ccgccttcga ccagccagag ctggtgcaga acctggtgga
      421 acggctctac cgccgttacg aattgggata tggcacacaa atgatgcccc gtggcctgaa
      481 agccctggaa acttcttcta ccgaaactta tgtaagcggt acaattgcca tcgatgcaga
      541 taacaatgaa attatcaata tgcctttcat tacctgcttc ctgtttacct gtataaaagg
      601 gaaaagtgaa ccctaccagt tgatatggag cagctcattg aactaggtcc gtttttagtc
      661 ggtactctga gaataaaaga aatggcaact aatgtgaatg ccggcgtttg atgatgcccc
      721 cggacgcctc cttaatagta tttgcaaaaa caaaagcctt ggggtccaca tcataaacta
      781 gattctttag tttccgcagt tccagtcttg ttattactgt gaaaataata tcacactctg
      841 cacttacatc atatttgccg ggcagaaagc ctctttcgcc tttgtaaaca gtaatacccc
      901 gtccgagttt gtttaccagc tggtatttaa tggcctcact tttaccagat acaatggtaa
      961 caccggtata ggcctgcaaa ccctctacca cataatcaat acaacgggtg gctgtaaagt
     1021 atgtcagaat cgaatacaag gcagtttcaa ttccaaaacg aaaccctgca atggtgaaaa
     1081 taattatatt gatacccagt atgatctctg tgatggtaaa agaggtacgc ttcagggtat
     1141 acaccgccag tacttctatc ccgtcgaggg ctgccccagt tctcatgata agcccaacgc
     1201 caatgccaag gaaagctccg ccgaaaattg agatgagtaa tttgtcggct gtcatggc
//
LOCUS       DAORLS010000486         1241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_52011_length_1241_cov_1.523609, whole genome shotgun sequence.
ACCESSION   DAORLS010000486 DAORLS010000000
VERSION     DAORLS010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52011_length_1241_cov_1.523609"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PK977_20260"
     CDS             <1..730
                     /locus_tag="PK977_20260"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRF20530.1"
                     /translation="RNFGMKVNADAFEAMSRSIPITILAKQKQQIHQLEALLLGQAGL
                     LEGKLKDEYAVMLQKEYRYQKVKYKLKPIIIPLHFLRMRPVNFPTVRLAQLAMLVHQS
                     AHLFSKIKEAGAVKEIKNWFDVTANDYWHYHYRFDESSSFKKKKLGEAMINNIIINTV
                     APVLFAYGSYHNEQQYKDKALLWLEGTAPEANSITKGFTGLGIENKNAFDSQVLIEMK
                     NEYCNKKRCLECGVGNALLKQNKE"
     gene            complement(778..1200)
                     /locus_tag="PK977_20265"
     CDS             complement(778..1200)
                     /locus_tag="PK977_20265"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRF20531.1"
                     /translation="MTSSVVYTGDLRTTCTHLRSHSSFETDAPLDNNGKGERFSPTDL
                     MATSLATCMITVMGIKARTMGFDLNDVKIDVLKIMKAEPRRVGGIELTFHIPDALKNI
                     DEKTKAILRNTGETCPVMKSIHPDIEVKVDWGVWGSEF"
BASE COUNT          370 a          251 c          277 g          343 t
ORIGIN      
        1 ccgcaatttt ggtatgaagg tgaatgccga tgcttttgaa gccatgtccc gttctattcc
       61 cattactatc ctggccaagc aaaagcagca gatacaccag ttagaagccc tgttactggg
      121 gcaggctggt ttgctcgaag ggaaattgaa agatgagtat gcggtgatgt tgcaaaagga
      181 gtaccgttac caaaaggtca agtataagct caaaccaata atcatccctt tacattttct
      241 caggatgagg ccggtaaatt ttccaactgt acgattggca caacttgcta tgctggtaca
      301 ccagtcggct catttgtttt ccaaaataaa agaggcaggc gctgtaaagg agatcaaaaa
      361 ctggtttgac gtgacggcca acgattactg gcattatcat tatcgctttg atgaatcatc
      421 atcttttaaa aagaaaaaac tgggcgaagc gatgattaat aatatcatca ttaatactgt
      481 tgcacctgta ctatttgctt atggtagtta tcataacgaa cagcagtata aagacaaggc
      541 cctgttatgg ctggaaggca cagcgccgga ggccaacagt attaccaaag ggtttaccgg
      601 gttaggtatt gaaaataaga atgcctttga cagccaggtg ttgatagaaa tgaaaaatga
      661 atattgcaat aagaaaagat gtttggagtg cggggtggga aatgctttgt taaagcaaaa
      721 taaagaatag taaaagtgta aattttttag tcgcactaaa cactcccgac ttgccactca
      781 aaattcacta ccccacactc cccaatccac ctttacctca atatccgggt gaatgctttt
      841 catgacgggg caggtttcac cggtatttct caggatggct ttcgtttttt cgtctatgtt
      901 ctttaatgca tcgggtatat gaaaggtcag ttcaatgcca cccacacggc ggggttcagc
      961 tttcatgatc tttagcacat caatcttcac atcattcagg tcaaaaccca ttgttctggc
     1021 cttaatgccc ataacggtaa tcatacaggt agccaggctg gtagccatca ggtcagtggg
     1081 ggagaaccgt tctccttttc cattgttatc aaggggagca tctgtttcaa aagaagagtg
     1141 gctgcgcaaa tgggtacagg tggtgcgaag gtcgccggta tacactacgg aagaagtcat
     1201 tatcaatatt ttaccctaaa tttacgttta gtacattaaa a
//
LOCUS       DAORLS010000487         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_55802_length_1165_cov_2.714414, whole genome shotgun sequence.
ACCESSION   DAORLS010000487 DAORLS010000000
VERSION     DAORLS010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55802_length_1165_cov_2.714414"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..>1165)
                     /locus_tag="PK977_20270"
     CDS             complement(106..>1165)
                     /locus_tag="PK977_20270"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRF20532.1"
                     /translation="SFNYGNISDVYLRMGDHDKARQYAQLTLQITKQLNAPHRIINAC
                     NQLTRVFKKTGQFDSALYYKDWSVMLADSLNNLEKTAQIAEMQTKYEAGKKDAHIEEL
                     TELNEVKNQRFWLMAGASLVLMVLLFVVWNQKKKSQRQQKMIAEQSDRLQWMMKELHH
                     RVKNNLQIVSSLLNLQSYRLKDEESVSAIKESQLRVQAMSLMHQRLYQVEDVSMVNFK
                     LYLTDLTETLMRAYGYCSDDFDLTIDIEKEMLDVDTVMPMGLLVNEIITNSFKYAFKE
                     VKRPALRIKLGGNDRNLVLDIADNGPGMQSAGTTKAGFGTKLISALSKQLKATYTVDL
                     SAGTAYSFTIPYTHKKAA"
BASE COUNT          310 a          278 c          214 g          363 t
ORIGIN      
        1 aataccagcc actttgtaac catcatgctc aagcccggcg gccaggtcca tggctacaat
       61 ggattcatct tctacaatca ttacatgtac ctggttgggc ataggtcagg ctgctttttt
      121 atgagtataa ggaatagtaa acgaataagc cgttcctgct gagagatcaa cagtataggt
      181 ggccttcagc tgtttactca atgctgaaat aagttttgtt ccaaaaccag ctttagtagt
      241 acctgctgat tgcatacccg gcccgttatc tgcaatatcg agtacaagat ttctatcatt
      301 ccctccaagc ttaattcgaa gagccggacg ttttacttct ttaaatgcat acttaaagga
      361 attagtgatg atctcgttca caagcaagcc cattggcata accgtatcta catcaagcat
      421 ttctttttcg atatcgatcg taagatcaaa atcatccgaa caataaccat aagccctcat
      481 cagtgtttca gtaagatctg tcaggtatag cttaaagttc accatgctaa catcctccac
      541 ctggtaaagc cgctgatgca tcagcgacat agcctgcacc cgcaactggc tctccttgat
      601 agcagaaaca ctttcttcat ctttcagccg gtagctttgc aggttcagca ggcttgaaac
      661 gatctgcagg ttattcttta cccggtggtg cagctctttc atcatccatt gcaaacggtc
      721 tgattgttcg gctatcattt tttgttgccg ctgcgatttc ttcttttgat tccagacaac
      781 aaataataaa accatcaata caagtgatgc ccctgccatt aaccaaaacc gctggttctt
      841 tacttcattc agttcagtta gctcctcgat atgcgcatct ttcttgccgg cttcatactt
      901 tgtctgcatt tcagcaatct gtgctgtctt ctccaggtta ttcagtgaat cggccagcat
      961 caccgaccag tctttataat aaagggcaga atcaaactgg cctgtttttt taaataccct
     1021 tgttaattga ttacaggcat taataatccg gtgaggcgca tttaattgct ttgttatttg
     1081 cagcgtaagt tgtgcatact gtcttgcctt atcatgatca cccattcgaa gatatacatc
     1141 cgaaatatta ccatagttga aggaa
//
LOCUS       DAORLS010000488         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_57944_length_1127_cov_2.013060, whole genome shotgun sequence.
ACCESSION   DAORLS010000488 DAORLS010000000
VERSION     DAORLS010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57944_length_1127_cov_2.013060"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..837)
                     /locus_tag="PK977_20275"
     CDS             complement(154..837)
                     /locus_tag="PK977_20275"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRF20533.1"
                     /translation="MKRAGFVPSAMELVEINALKIVSKFVDSNAIPITDDTAAHLIIE
                     VDGNNRDVLMQEMEAIGNLLTQYDIGEIYFADDAQQKAELWKLRRRAAEAVKMIGYTI
                     EEDTVVPRAELPALIKGVKELAVKHDFDVVCYGHAGDGNLHVRIRKEGIPNSHGNAEM
                     TEVLRELFKLVKQLGGTISAEHGIGLIQKPYMDIAFNDPALRLMREIKKVFDPNNILN
                     PGKIFDLKN"
     gene            complement(788..>1127)
                     /locus_tag="PK977_20280"
     CDS             complement(788..>1127)
                     /locus_tag="PK977_20280"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRF20534.1"
                     /translation="SCFIGGNIAENSGGPKAVKYGVVKDYVLNLEVVLPTGEIIWTGS
                     NVIKNATGYNLTQLIVGSEGTLGVVTKIVLRLIPLPQFDLLMLVPFRNLEQAGEAGRV
                     CSQRHGTGRD"
BASE COUNT          306 a          287 c          180 g          354 t
ORIGIN      
        1 attgttgttc cgctaaaaaa tgatacggtt acactgccac ctgtaaacag gttttccttt
       61 ttgaatccct tttcttcatc gttctgggca aatgcaacag aaacaacagc agtcatcagc
      121 attgaaaaaa taagtttctt catataatag ggtttagttc tttagatcaa atattttccc
      181 ggggtttaaa atattattcg gatcaaatac cttttttatt tctcgcatta aacgcagggc
      241 aggatcatta aacgcaatgt ccatataagg tttttgaata aggccaatac cgtgctctgc
      301 actaattgta ccgcctagtt gttttacaag tttaaaaagc tcccttagca cttccgtcat
      361 ctcagcatta ccatgactat tcgggatacc ttcttttctt attcgtacat gcaaatttcc
      421 atctcctgca tgcccgtaac aaaccacatc aaagtcatgc tttactgcca gctcttttac
      481 tccttttatc aaagccggta attcggctct tggaacaacc gtatcttctt cgatagtata
      541 accaatcatc ttcactgctt cagccgccct ccttctcagc ttccacaatt ctgctttttg
      601 ctgcgcatcg tctgcaaaat aaatttcacc aatatcatat tgtgttaaca ggttgccaat
      661 tgcctccatc tcctgcatca atacatccct attatttccg tccacttcaa ttataaggtg
      721 ggctgctgta tcatcagtaa tggggatagc gttactgtct acgaatttgc tgacaatctt
      781 taaggcatta atctcgacca gttccatggc gctgggaaca aacccggccc gcttcaccag
      841 cctgctcaag attccggaaa ggcaccagca tcagcagatc aaattgtggc agaggtatta
      901 atctcaatac gatttttgta acaacgccta atgttccctc acttcctact atcagctgtg
      961 tgagattata cccggttgca ttcttgatca catttgaacc tgtccaaata atctcaccag
     1021 tgggcaatac gacctcaaga ttcaacacat aatctttcac cacaccatac ttcaccgctt
     1081 tggggccacc actgttttcg gcaatatttc ctccaataaa gcaggag
//
LOCUS       DAORLS010000489         1057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5493
            NODE_62261_length_1057_cov_2.424152, whole genome shotgun sequence.
ACCESSION   DAORLS010000489 DAORLS010000000
VERSION     DAORLS010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867710
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:17:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,057
            CDSs (total)                      :: 4,042
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 2 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62261_length_1057_cov_2.424152"
                     /isolate="AMR_MDS_5493"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1057)
                     /locus_tag="PK977_20285"
     CDS             complement(<1..>1057)
                     /locus_tag="PK977_20285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF20535.1"
                     /translation="HYQFFFNQLIGKKFTFHTALFLTKGKGYYEQYKADEVYADYGFP
                     DPLPAGGINITNSDMVRQLWLDNDFYGNIFSLQYKTDKTQATLGGGVTRYDGNHFGKV
                     IWAEKGLTSVDNWYDLDAKKTDFNVYLKQQTEFAEHWYGFYDLQFRSAQYIIEGFRNN
                     PGLFIDNTFNFFNPKAGISYSRNNWKGYASFGIANKEPNRDDFEAGAAQQPRPERLQD
                     IEVGIERTGKNFNWSATFYYMNYKDQLVLTGKINDVGAYTRTNIPNSYRTGVELQGAC
                     KFSEWMNATANLALSRNKVKNFTEYIDDYDNGGQKEISYTSSDIAFSPSIVGGAGINF
                     LPVKKGELSLLSKYVSK"
BASE COUNT          268 a          233 c          210 g          346 t
ORIGIN      
        1 tgtttgctta catatttgct cagcaggctc aattcccctt ttttcaccgg taaaaaatta
       61 atacctgctc cgccaacaat tgaaggggag aaggcaatat cagaagaagt gtaactgatc
      121 tctttttgtc ctccattatc atagtcatca atatactccg taaaattctt cactttatta
      181 cggctcagtg ccaggttagc tgttgcattc atccattcgc tgaatttaca ggcaccctgt
      241 aattctacgc cggtcctgta gctattagga atgttggtac gggtgtatgc acccacatca
      301 ttgatcttgc cggttagcac taactggtct ttgtagttca tgtaatagaa tgtggcagac
      361 cagttgaaat tttttccagt acgttcaata cccacctcaa tatcctgcaa tctttccggc
      421 ctgggttgtt gtgctgcccc ggcttcaaaa tcatcccggt taggttcttt gttggctatg
      481 ccaaaggaag catacccttt ccagttgttg cggctgtagc tgattcctgc ttttgggttg
      541 aagaaattaa atgtattatc aatgaacaaa ccggggttat tgcggaagcc ttcaataatg
      601 tattgggcac tacggaactg caggtcgtaa aacccgtacc agtgttctgc aaattctgtt
      661 tgttgcttca ggtatacatt aaagtctgtt ttcttcgcat cgaggtcata ccagttgtca
      721 acagaggtca atcctttttc tgcccagatc actttaccga agtgatttcc atcataccgg
      781 gtaactcctc cgccaagtgt tgcctgtgtt ttatctgttt tgtactgcag cgaaaaaata
      841 ttgccataaa aatcattgtc cagccatagc tgtcttacca tatcagaatt ggtaatgttt
      901 attccccctg ctggtaacgg gtccggaaaa ccataatcgg catatacttc atctgccttg
      961 tattgttcat aataaccttt tcctttcgtt agaaataaag ctgtgtggaa agtgaatttt
     1021 ttaccaatca gctggttgaa gaaaaactgg taatgtg
//
